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Sample records for cactaceae

  1. Cactaceae

    NARCIS (Netherlands)

    Stoffers, A.L.

    1984-01-01

    Stemsucculents, branched or unbranched, columnar, globose or with thick flattened stems or internodes, usually with very small scale-like and awl-shaped, rarely foliaceous leaves; stems and internodes often with warts or ribs, bearing areoles in the axils of the often absent leaves. Areoles mostly c

  2. Cactaceae do Vale do Rio Jequitinhonha (Minas Gerais Cactaceae of Jequitinhonha river valley (Minas Gerais

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    Nigel P Taylor

    1991-07-01

    Full Text Available Durante as primeiras coletas relacionadas ao Projeto "Cactaceae no Brasil Oriental", ficou provada a grande diversidade de espércies dessa família na região do vale médio do Rio Jequitinhonha, tendo sido visitadas as localidades de Itinga, Itaobim e Pedra Azul. Foram coletadas 21 espécies distribuídas entre os gêneros Pereskia, Opuntia, Tacinga, Pseudoacanthocereus, Arrojadoa, Brasilicereus, Cereus, Coleocephalocereus, Melocactus, Pilosocereus e Selenicereus, representando as três subfamílias de Cactaceae, cujas afinidades taxonômicas e padrões de distribuição geográfica são discutidos a seguir.During an initial visit to collect material for the projected "Cacti of Eastern Brazil", a remarkable great diversity of species of Cactaceae in the region of middle drainage of the Rio Jequitinhonha (mainly in the localities of Itinga, Itaobim and Pedra Azul was noted. Twenty one species in the genera Pereskia, Opuntia, Tacinga, Pseudocanthocereus, Arrojadoa, Brasilicereus, Cereus, Coloecephalocereus, Melocactus, Pilosocereus e Selenicereus representing all three subfamilies of Cactaceae were recorted. The phytogeographic affinities of the Cactaceae from this region are discussed.

  3. Further naturalised Cactaceae in northeastern Iberian Peninsula

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    Dana Sánchez, Elías D.

    2006-06-01

    Full Text Available During the floristic work carried out in 2004 in the Baix Camp region of Tarragona (NE Spain we found four new species of alien naturalised Cactaceae. Of these, three are reported for the second time from Europe and the other is new to Catalonia region. Southern Catalonia, with eleven species of Cactaceae, harbours the highest diversity of the family in Europe. Our results demonstrate that this diversity is even higher than previously believed. The introduction of these exotic taxa is, once again, due to the development of gardening based exclusively on foreign elements, to the unscrupulous and uncontrolled dumping of their remains in the natural environment, and to the existence of a favourable climate.Durante la campaña de prospección florística llevada a cabo en 2004 por la comarca del Baix Camp (Tarragona, NE de España hemos encontrado cuatro especies de cactáceas alóctonas naturalizadas. De ellas, tres se citan por segunda vez de Europa y la otra es nueva para Cataluña. Nuestros resultados aumentan aún más la diversidad de cactáceas del sur de Cataluña que, con once especies, es la mayor de toda Europa. La introducción de estos táxones exóticos que ahora damos a conocer se debe, una vez más, al desarrollo de una jardinería basada casi exclusivamente en elementos foráneos, en el vertido desaprensivo e incontrolado de sus restos en el medio natural y en la existencia de una climatología muy favorable.

  4. Anatomical investigation on Cactaceae Juss.: a historical retrospect

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    Galina V. Kalashnyk

    2014-04-01

    Full Text Available Cactaceae consists of perennial stem succulents with diverse morphology. Due the distinctive structure and form the interest of researchers to this group of plants is increasing nowadays. The present paper provides an overview of published data concerning anatomical studies on the family Cactaceae since the mid-nineteenth century to our days. It is important to consider that recent interest in this field does not reduce, while the number of studies dealing with the structure and features of seedlings, the effect of various environmental factors on them is uprising. Such studies have a great practical importance for introduction and reintroduction of cacti, as well as for determination of their adaptive characteristics to environmental conditions.

  5. A Novel Method of Genomic DNA Extraction for Cactaceae

    OpenAIRE

    Fehlberg, Shannon D.; Jessica M. Allen; Kathleen Church

    2013-01-01

    • Premise of the study: Genetic studies of Cactaceae can at times be impeded by difficult sampling logistics and/or high mucilage content in tissues. Simplifying sampling and DNA isolation through the use of cactus spines has not previously been investigated. • Methods and Results: Several protocols for extracting DNA from spines were tested and modified to maximize yield, amplification, and sequencing. Sampling of and extraction from spines resulted in a simplified protocol overall and compl...

  6. Use and knowledge of Cactaceae in Northeastern Brazil

    Science.gov (United States)

    2013-01-01

    Background This study aimed to record the use, and knowledge that residents from São Francisco community (Paraiba, Brazil) have regarding the Cactaceae. Methods Semi-structured interviews were carried out with 118 informants; 50 men and 68 women. The cacti cited in this study were organised into use categories and use values were calculated. Differences in the values applied to species and use categories by men and women were compared via a G test (Williams). Results The nine species identified were: Cereus jamacaru DC., Melocactus bahiensis (Brtitton & Rose) Luetzelb., Nopalea cochenillifera (L.) Salm-Dyck., Opuntia ficus indica (L.) Mill, Opuntia stricta (Haw.) Haw., Pilosocereus gounellei (F.A.C. Weber) Byles & Rowley, Pilosocereus pachycladus F. Ritter, Tacinga inamoena (K. Schum) N.P. Taylor & Stuppy, Tacinga palmadora (Britton & Rose) N.P. Taylor & Stuppy. In total, 1,129 use citations were recorded, divided into 11 categories. The use value categories with the highest scores were forage (0.42), food (0.30) and construction (building) (0.25). P. pachycladus showed the greatest use value, versatility and number of plant parts used. Conclusion The survey showed that the Cactaceae is extremely important for several uses and categories attributed to different species. Apart from contributing to the ethnobotanical knowledge of the Cactaceae, another important focus of this study was to reinforce the necessity for further studies that record the traditional knowledge about this plant family, which has been lost in younger generations. PMID:23981911

  7. Characterization of calcium oxalate biominerals in Pereskia species (Cactaceae).

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    Monje, Paula V; Baran, Enrique J

    2009-01-01

    Calcium oxalate druses were isolated from the stems and leaves of six Pereskioideae family members and investigated by infrared spectroscopy, showing that in all samples the biomineral was present in the form of whewellite, CaC2O4 x H2O. As Pereskia is thought to represent the "ancestral" condition of the leafless stem-succulent cacti, these results suggest that the biomineralization of calcium oxalate in Cactaceae represents a primitive characteristic of the group and also support a close genetic relationship between Pereskia and Opuntia.

  8. The polymorphic weddellite crystals in three species of Cephalocereus (Cactaceae).

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    Bárcenas-Argüello, María-Luisa; Gutiérrez-Castorena, Ma C-del-Carmen; Terrazas, Teresa

    2015-10-01

    Mineral inclusions in plant cells are genetically regulated, have an ecological function and are used as taxonomic characters. In Cactaceae, crystals in epidermal and cortical tissues have been reported; however, few studies have conducted chemical and morphological analyses on these crystals, and even fewer have reported non-mineral calcium to determine its systematic value. Cephalocereus apicicephalium, C. totolapensis and C. nizandensis are Cactaceae species endemic to the Isthmus of Tehuantepec, Mexico with abundant epidermal prismatic crystals. In the present study, we characterize the mineral cell inclusions, including their chemical composition and their morphology, for three species of Cephalocereus. Crystals of healthy branches of the three species were isolated and studied. The crystals were identified by X-ray diffraction (XRD), their morphology was described using a petrographic and scanning electron microscope (SEM), and their elemental composition was measured with Energy Dispersive X-ray (EDXAR). The three species synthesized weddellite with different degrees of hydration depending on the species. The optical properties of calcium oxalate crystals were different from the core, which was calcium carbonate. We observed a large diversity of predominantly spherical forms with SEM. EDXAR analysis detected different concentrations of Ca and significant amounts of elements, such as Si, Mg, Na, K, Cl, and Fe, which may be related to the edaphic environment of these cacti. The occurrence of weddellite is novel for the genus according to previous reports. The morphological diversity of the crystals may be related to their elemental composition and may be a source of phylogenetic characters.

  9. A novel method of genomic DNA extraction for Cactaceae1

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    Fehlberg, Shannon D.; Allen, Jessica M.; Church, Kathleen

    2013-01-01

    • Premise of the study: Genetic studies of Cactaceae can at times be impeded by difficult sampling logistics and/or high mucilage content in tissues. Simplifying sampling and DNA isolation through the use of cactus spines has not previously been investigated. • Methods and Results: Several protocols for extracting DNA from spines were tested and modified to maximize yield, amplification, and sequencing. Sampling of and extraction from spines resulted in a simplified protocol overall and complete avoidance of mucilage as compared to typical tissue extractions. Sequences from one nuclear and three plastid regions were obtained across eight genera and 20 species of cacti using DNA extracted from spines. • Conclusions: Genomic DNA useful for amplification and sequencing can be obtained from cactus spines. The protocols described here are valuable for any cactus species, but are particularly useful for investigators interested in sampling living collections, extensive field sampling, and/or conservation genetic studies. PMID:25202521

  10. A Novel Method of Genomic DNA Extraction for Cactaceae

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    Shannon D. Fehlberg

    2013-03-01

    Full Text Available Premise of the study: Genetic studies of Cactaceae can at times be impeded by difficult sampling logistics and/or high mucilage content in tissues. Simplifying sampling and DNA isolation through the use of cactus spines has not previously been investigated. Methods and Results: Several protocols for extracting DNA from spines were tested and modified to maximize yield, amplification, and sequencing. Sampling of and extraction from spines resulted in a simplified protocol overall and complete avoidance of mucilage as compared to typical tissue extractions. Sequences from one nuclear and three plastid regions were obtained across eight genera and 20 species of cacti using DNA extracted from spines. Conclusions: Genomic DNA useful for amplification and sequencing can be obtained from cactus spines. The protocols described here are valuable for any cactus species, but are particularly useful for investigators interested in sampling living collections, extensive field sampling, and/or conservation genetic studies.

  11. Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae) leaves.

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    Malek, Sri Nurestri Abdul; Shin, Sim Kae; Wahab, Norhanom Abdul; Yaacob, Hashim

    2009-05-06

    Dihydroactinidiolide (1) and a mixture of sterols [campesterol (2), stigmasterol (3) and beta-sitosterol (4)], together with the previously isolated individual compounds beta-sitosterol (4), 2,4-di-tert-butylphenol (5), alpha-tocopherol (6), phytol (7) were isolated from the active ethyl acetate fraction of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Cytotoxic activities of the above mentioned compounds against five human carcinoma cell lines, namely the human nasopharyngeal epidermoid carcinoma cell line (KB), human cervical carcinoma cell line (CasKi), human colon carcinoma cell line (HCT 116), human hormone-dependent breast carcinoma cell line (MCF7) and human lung carcinoma cell line (A549); and non-cancer human fibroblast cell line (MRC-5) were investigated. Compound 5 possessed very remarkable cytotoxic activity against KB cells, with an IC(50 )value of 0.81microg/mL. This is the first report on the cytotoxic activities of the compounds isolated from Pereskia bleo.

  12. Level of environmental threat posed by horticultural trade in Cactaceae.

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    Novoa, Ana; Le Roux, Johannes J; Richardson, David M; Wilson, John R U

    2017-01-11

    Ornamental horticulture has been identified as an important threat to plant biodiversity and the major pathway for plant invasions worldwide. In this context, the family Cactaceae is particularly interesting and challenging for three main reasons-it is considered the fifth most threatened major taxonomic group in the world; several cactus species are amongst the most widespread and damaging invasive species; and Cactaceae is one of the most popular horticultural plant groups. Based on CITES trade data and the eleven main auction sites selling cacti on the internet we document a substantial global trade from and to almost all continents. While less than 20 % of this trade involves threatened species, and less than 3% involves known invasive species, many species are sold without a valid scientific name. Importantly, however, hardly any of the globally traded cacti are collected from wild populations. In order to provide an in-depth look at the dynamics of the industry, we surveyed the businesses involved in the cactus trade in South Africa (one of the main hotspots of cactus trade and invasions). Despite a large commercial network, all South African imports (of which only 15 % and 1.5 % were of species listed as threatened and invasive, respectively) came from the same source. We purchased seeds of every available species and, based on DNA-barcoding techniques, could only identify 24 % of the species to genus level. If trade restrictions are placed on the small proportion of cacti that are invasive and there is no major increase in harvesting of native populations, the commercial cactus horticultural trade will pose a negligible environmental threat. However, there are currently no effective methods for easily identifying which cacti are traded, and both the illicit harvesting of cacti from the wild and the informal trade in invasive taxa pose on-going conservation challenges. This article is protected by copyright. All rights reserved.

  13. Flora do Pico do Jabre, Paraíba, Brasil: Cactaceae juss Flora of the Pico do Jabre, Paraíba, Brazil: Cactaceae juss

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    Emerson Antonio Rocha

    2002-01-01

    Full Text Available Neste trabalho realizou-se o tratamento sistemático das Cactaceae do Pico do Jabre, Paraíba, Brasil. A área de estudo é o ponto mais alto do Nordeste setentrional, constitui-se de um enclave de mata serrana, sendo considerada um dos poucos representantes da Paraíba incluídos no domínio da Mata Atlântica. No Pico do Jabre, as Cactaceae estão representadas por três gêneros e quatro espécies: Cereus jamacaru DC., Melocactus ernestii Vaupel, Pilosocereus chrysostele (VaupelByles & Rowley e Pilosocereus gounellei (F.A.C.WeberByles & Rowley.This work constitutes a systematic treatment of the Cactaceae family found in the "Pico do Jabre", Paraíba, Brazil. The study-area is the highest peak in the Northern part of Northeastern Brazil and consists of an isolated portion of humid forest, one of the few in the State of Paraíba, included in the the domain of the Atlantic Forest. In the "Pico do Jabre" the Cactaceae family is represented by three genera and four species: Cereus jamacaru DC., Melocactus ernestii Vaupel, Pilosocereus chrysostele (Vaupel Byles & Rowley and Pilosocereus gounellei (F.A.C.WeberByles & Rowley.

  14. Non-concerted ITS evolution in Mammillaria (Cactaceae).

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    Harpke, Doerte; Peterson, Angela

    2006-12-01

    Molecular studies of 21 species of the large Cactaceae genus Mammillaria representing a variety of intrageneric taxonomic levels revealed a high degree of intra-individual polymorphism of the internal transcribed spacer region (ITS1, 5.8S rDNA, ITS2). Only a few of these ITS copies belong to apparently functional genes, whereas most are probably non-functional (pseudogenes). As a multiple gene family, the ITS region is subjected to concerted evolution. However, the high degree of intra-individual polymorphism of up to 36% in ITS1 and up to 35% in ITS2 suggests a non-concerted evolution of these loci in Mammillaria. Conserved angiosperm motifs of ITS1 and ITS2 were compared between genomic and cDNA ITS clones of Mammillaria. Some of these motifs (e.g., ITS1 motif 1, 'TGGT' within ITS2) in combination with the determination of GC-content, length comparisons of the spacers and ITS2 secondary structure (helices II and III) are helpful in the identification of pseudogene rDNA regions.

  15. Cytotoxic Components of Pereskia bleo (Kunth DC. (Cactaceae Leaves

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    Sri Nurestri Abdul Malek

    2009-05-01

    Full Text Available Dihydroactinidiolide (1 and a mixture of sterols [campesterol (2, stigmasterol (3 and β-sitosterol (4], together with the previously isolated individual compounds β-sitosterol (4, 2,4-di-tert-butylphenol (5, α-tocopherol (6, phytol (7 were isolated from the active ethyl acetate fraction of Pereskia bleo (Kunth DC. (Cactaceae leaves. Cytotoxic activities of the above mentioned compounds against five human carcinoma cell lines, namely the human nasopharyngeal epidermoid carcinoma cell line (KB, human cervical carcinoma cell line (CasKi, human colon carcinoma cell line (HCT 116, human hormone-dependent breast carcinoma cell line (MCF7 and human lung carcinoma cell line (A549; and non-cancer human fibroblast cell line (MRC-5 were investigated. Compound 5 possessed very remarkable cytotoxic activity against KB cells, with an IC50 value of 0.81µg/mL. This is the first report on the cytotoxic activities of the compounds isolated from Pereskia bleo.

  16. Gynogenesis in the vine cacti Hylocereus and Selenicereus (Cactaceae).

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    Garcia, Reinerio Benega; Cisneros, Aroldo; Schneider, Bert; Tel-Zur, Noemi

    2009-05-01

    Gynogenesis was investigated on the allotetraploid Selenicereus megalanthus and the diploid Hylocereus polyrhizus and Hylocereus undatus vine cactus species. Unpollinated ovules from developing flower buds containing microspores at middle uninucleate developmental stage were cultured on MS basal medium containing 2,4-D/TDZ with different sucrose concentrations. Ovule size increased under dark culture conditions in all the three species and the level of response was species and sucrose concentration dependent. The best responses were achieved in the two S. megalanthus accessions, E-123 and J-80, at 0.18 and 0.26 M sucrose. Only ovule enlargement was obtained in H. undatus and both ovule enlargement and callus were obtained in H. polyrhizus. Development in both species ceased and embryoids were not formed. Plant regeneration was directly and indirectly obtained in both S. megalanthus accessions. Ploidy level was determined for a total of 29 S. megalanthus gynogenic plants using flow cytometry: 15 were found to be dihaploid (plants with the gametophytic chromosome number) and the other 14 were found to have higher ploidy levels. This is the first report of successful gynogenesis in Cactaceae. The dihaploids of S. megalanthus successfully produced by ovule culture techniques opens new perspectives in vine cacti breeding.

  17. Evidence of formation of glushinskite as a biomineral in a Cactaceae species.

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    Monje, Paula V; Baran, Enrique J

    2005-03-01

    The X-ray diffractometric and infrared spectroscopic investigation of crystalline material isolated from the Cactaceae species Opuntia ellisiana shows the presence of a very complex mineral composition, including whewellite (monohydrated calcium oxalate), opal (SiO2), calcite (CaCO3) and glushinskite (dihydrated magnesium oxalate). This is the first report of the presence of magnesium oxalate in plants.

  18. Population genetic structure of Venezuelan chiropterophilous columnar cacti (Cactaceae).

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    Nassar, Jafet M; Hamrick, J L; Fleming, Theodore H

    2003-11-01

    We conducted allozyme surveys of three Venezuelan self-incompatible chiropterophilous columnar cacti: two diploid species, Stenocereus griseus and Cereus repandus, and one tetraploid, Pilosocereus lanuginosus. The three cacti are pollinated by bats, and both bats and birds disperse seeds. Population sampling comprised two spatial scales: all Venezuelan arid zones (macrogeographic) and two arid regions in northwestern Venezuela (regional). Ten to 15 populations and 17-23 loci were analyzed per species. Estimates of genetic diversity were compared with those of other allozyme surveys in the Cactaceae to examine how bat-mediated gene dispersal affects the population genetic attributes of the three cacti. Genetic diversity was high for both diploid (P(s) = 94.1-100, P(p) = 56.7-72.3, H(s) = 0.182-0.242, H(p) = 0.161-0.205) and tetraploid (P(s) = 93.1, P(p) = 76.1, H(s) = 0.274, H(p) = 0.253) species. Within-population heterozygote deficit was detected in the three cacti at macrogeographic (F(IS) = 0.145-0.182) and regional (F(IS) = 0.057-0.174) levels. Low genetic differentiation was detected at both macrogeographic (G(ST) = 0.043-0.126) and regional (G(ST) = 0.009-0.061) levels for the three species, suggesting substantial gene flow among populations. Gene exchange among populations seems to be regulated by distance among populations. Our results support the hypothesis that bat-mediated gene dispersal confers high levels of genetic exchange among populations of the three columnar cacti, a process that enhances levels of genetic diversity within their populations.

  19. Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae

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    T.C.F.R. Aragão

    2000-08-01

    Full Text Available We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae, to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa and a heavy chain (7.63 kDa. This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.

  20. Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts

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    Sim, K. S.; A M Sri Nurestri; Norhanom, A. W.

    2010-01-01

    The leaves of Pereskia grandifolia Haw. (Cactaceae), commonly known as “Jarum Tujuh Bilah” in Malaysia, have been traditionally used as natural remedy in folk medicine by the locals. In the present study, the antioxidant potential of P. grandifolia crude methanol and its fractionated extracts (hexane, ethyl acetate and water) have been investigated, employing three different established testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing pow...

  1. AVALIAÇÃO DAS PROPRIEDADES ANTIOXIDANTES E SUSCEPTIBILIDADE ANTIMICROBIANA DE Pereskia grandifolia HAWORTH (CACTACEAE)

    OpenAIRE

    Turra, Andersson Franklin; Marçal, Flaviano João Basílio; Baretta, Irinéia Paulina; Takemura, Orlando Seiko; Jr, Antonio Laverde

    2008-01-01

    A espécie Pereskia grandifolia Haworth (Cactaceae), conhecida popularmente como ora-pro-nóbis, foi avaliada quanto àssuas propriedades antioxidantes e susceptibilidade antimicrobiana. As propriedades antioxidantes foram avaliadas pelo método de inibiçãodo radical livre 2,2-difenil-1-picrilidrazil (DPPH•). Os extratos das folhas de P. grandifolia (1000 ìg/mL) apresentaram propriedadesantioxidantes entre 10 e 30% de inibição. D...

  2. Functional morphology of two Lepismium species (Rhipsalideae, Cactaceae Morfología funcional de dos especies de Lepismium (Rhipsalideae, Cactaceae

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    María Regina Torres-Boeger

    2010-08-01

    Full Text Available The morphology and anatomy of stem segments of 2 species of Lepismium (Cactaceae, which grow naturally in the Araucaria forest understory, in the state of Paraná, Brazil, are compared. The goal of this study was to identify morphological traits adapted to epiphytism and to the low light condition of the studied environment. Twenty-five segments of Lepismium cruciforme and L. lumbricoides were collected and various morphological and anatomical features were measured. Differences (p Se compara la morfología y la anatomía de los segmentos del tallo de 2 especies de Lepismium, que crecen naturalmente en el sotobosque del bosque de Araucaria, en el estado de Paraná. El objetivo de este estudio fue identificar rasgos morfológicos de adaptación para el epifitismo y para condiciones de poca luz del ambiente estudiado. Se recolectaron 25 ejemplares de L. cruciforme y de L. lumbricoides y se midieron rasgos tanto morfológicos como anatómicos de los tallos. Se encontraron diferencias (p < 0.05 entre las especies considerando los valores promedio del volumen total, del área total fotosintética, del grosor de la epidermis y de la hipodermis, del área del esclerénquima / área transversal total de los segmentos del tallo y del área del parénquima / área transversal total, caracteres que se correlacionan a sus diferencias en forma. Las características xeromórficas encontradas en Lepismium, la mayor parte de ellas típicas de cactáceas de zonas áridas, han favorecido el desarrollo del hábito epifítico y la ocupación de bosques húmedos. Como epífitas, están sujetas a un cierto grado de escasez de agua, aunque no a las condiciones tan severas como la mayoría de las cactáceas terrestres. Los valores promedio para el grosor de la hipodermis, el contenido de agua, la cantidad de esclerénquima y las densidad estomática son más similares a las plantas mesomórficas, y se pueden correlacionar al epifitismo, demostrando que estas especies

  3. Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae

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    Itayguara Ribeiro da Costa

    2001-12-01

    Full Text Available We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae. This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed.A proteína de reserva mais abundante das sementes de Cereus jamacaru (Cactaceae foi isolada e caracterizada. Esta proteína tem uma massa molecular de 5319 kDa e foi isolada através de uma combinação de técnicas de filtração em gel e HPLC de fase reversa. A composição de aminoácidos da proteína foi determinada e possui similaridade com a composição de aminoácidos de diversas proteínas de reserva de sementes que pertencem à família das albuminas. A utilidade desta proteína como um marcador molecular para as cactáceas é também discutida.

  4. Cancer preventive and curative attributes of plants of the Cactaceae family: a review.

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    Harlev, Eli; Nevo, Eviatar; Solowey, Elaine; Bishayee, Anupam

    2013-06-01

    The ever-increasing occurrence of cancer and the severe side effects and limited efficacy of current cancer chemotherapy based on chemical drugs shift the attention toward drugs of plant origin. The Cactaceae family comprises more than 1500 species, but until recently only a few of them have been tested for their chemopreventive and anticancer attributes, leaving a wide unexplored area still waiting for researchers to investigate. Considering this fact, and also the promising results obtained with the relatively few plants of this family already tested, it should justly be expected that some plants of the Cactaceae family yet unexplored might possess outstanding anticancer attributes, exceeding those displayed by the plants already tested. This review presents in vitro and in vivo experimental evidence on cancer chemopreventive and therapeutic potential of bioactive phytoconstituents and extracts derived from cactus plants. It also examines the underlying biochemical and molecular mechanisms involved in the antineoplastic effects of plants of the Cactaceae family. Current limitation and future directions of research towards effective use of cacti to develop efficient and side effect-free future cancer-preventive and anticancer drugs are also discussed.

  5. Basal cactus phylogeny: implications of Pereskia (Cactaceae) paraphyly for the transition to the cactus life form.

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    Edwards, Erika J; Nyffeler, Reto; Donoghue, Michael J

    2005-07-01

    The cacti are well-known desert plants, widely recognized by their specialized growth form and essentially leafless condition. Pereskia, a group of 17 species with regular leaf development and function, is generally viewed as representing the "ancestral cactus," although its placement within Cactaceae has remained uncertain. Here we present a new hypothesis of phylogenetic relationships at the base of the Cactaceae, inferred from DNA sequence data from five gene regions representing all three plant genomes. Our data support a basal split in Cactaceae between a clade of eight Pereskia species, centered around the Caribbean basin, and all other cacti. Two other Pereskia clades, distributed mostly in the southern half of South America, are part of a major clade comprising Maihuenia plus Cactoideae, and Opuntioideae. This result highlights several events in the early evolution of the cacti. First, during the transition to stem-based photosynthesis, the evolution of stem stomata and delayed bark formation preceded the evolution of the stem cortex into a specialized photosynthetic tissue system. Second, the basal split in cacti separates a northern from an initially southern cactus clade, and the major cactus lineages probably originated in southern or west-central South America.

  6. The biological activities and chemical composition of Pereskia species (Cactaceae)--a review.

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    Pinto, Nícolas de Castro Campos; Scio, Elita

    2014-09-01

    The exploration of nature as a source of sustainable, novel bioactive substances continues to grow as natural products play a significant role in the search for new therapeutic and agricultural agents. In this context, plants of the genus Pereskia (Cactaceae) have been studied for their biological activities, and are evolving as an interesting subject in the search for new, bioactive compounds. These species are commonly used as human foodstuffs and in traditional medicine to treat a variety of diseases. This review focuses on the bioactivity and chemical composition of the genus Pereskia, and aims to stimulate further studies on the chemistry and biological potential of the genus.

  7. Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae

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    Uchoa A.F.

    1998-01-01

    Full Text Available We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae. This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.

  8. Regeneration of roots from callus reveals stability of the developmental program for determinate root growth in Sonoran Desert Cactaceae.

    Science.gov (United States)

    Shishkova, Svetlana; García-Mendoza, Edith; Castillo-Díaz, Vicente; Moreno, Norma E; Arellano, Jesús; Dubrovsky, Joseph G

    2007-05-01

    In some Sonoran Desert Cactaceae the primary root has a determinate root growth: the cells of the root apical meristem undergo only a few cell division cycles and then differentiate. The determinate growth of primary roots in Cactaceae was found in plants cultivated under various growth conditions, and could not be reverted by any treatment tested. The mechanisms involved in root meristem maintenance and determinate root growth in plants remain poorly understood. In this study, we have shown that roots regenerated from the callus of two Cactaceae species, Stenocereus gummosus and Ferocactus peninsulae, have a determinate growth pattern, similar to that of the primary root. To demonstrate this, a protocol for root regeneration from callus was established. The determinate growth pattern of roots regenerated from callus suggests that the program of root development is very stable in these species. These findings will permit future analysis of the role of certain Cactaceae genes in the determinate pattern of root growth via the regeneration of transgenic roots from transformed calli.

  9. Pollen Flavonoid/Phenolic Acid Composition of Four Species of Cactaceae and its Taxonomic Significance

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    Norma Almaraz-Abarca

    2008-01-01

    Full Text Available The pollen flavonoid/phenolic acid composition of 14 individual of Stenocactus multicostatus subsp. zacatecasensis (Britton et Rose U. Guzman et Vazquez-Benitez, comb. et stat. nov., 11 of Echinocereus enneacanthus Engelmann, 8 of Echinocereus pectinatus (Scheidweiler Engelmann and 12 of Mammillaria heyderi sensu lato (all belonging to Cactaceae was analyzed by HPLC/DAD with the aim of determining the role of pollen profiles of flavonoid/phenolic acid as valuable markers in those taxa. The flavonoid/phenolic acid composition of pollen was evaluated within and among the taxa analyzed. The results suggest that (a the flavonoid/phenolic acid profiles of these species are among the most complex reported, (b that quercetin, kaempferol and herbacetin glycoside derivatives are the major phenols found in the pollen of these species of cactus, (c that some intrapopulation variability is present in all four species and (d that these pollen flavonoid/phenolic acid profiles tend to be species-specific, so that they can represent important taxonomic markers in Cactaceae.

  10. Anatomical variation in Cactaceae and relatives: Trait lability and evolutionary innovation.

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    Ogburn, R Matthew; Edwards, Erika J

    2009-02-01

    The cacti have undergone extensive specialization in their evolutionary history, providing an excellent system in which to address large-scale questions of morphological and physiological adaptation. Recent molecular phylogenetic studies suggest that (1) Pereskia, the leafy genus long interpreted as the sister group of all other cacti, is likely paraphyletic, and (2) Cactaceae are nested within a paraphyletic Portulacaceae as a member of the "ACPT" clade (Anacampseroteae, Cactaceae, Portulaca, and Talinum). We collected new data on the vegetative anatomy of the ACPT clade and relatives to evaluate whether patterns in the distributions of traits may provide insight into early events in the evolutionary transition to the cactus life form. Many traits had high levels of homoplasy and were mostly equivocal with regard to infraclade relationships of ACPT, although several characters do lend further support to a paraphyletic Pereskia. These include a thick stem cuticle, prominent stem mucilage cells, and hypodermal calcium oxalate druses, all of which are likely to be important traits for stem water storage and photosynthesis. We hypothesize that high lability of many putative "precursor" traits may have been critical in generating the organismal context necessary for the evolution of an efficient and integrated photosynthetic stem.

  11. Wide-band tracheids are present in almost all species of Cactaceae.

    Science.gov (United States)

    Mauseth, James D

    2004-02-01

    Wide-band tracheids (WBTs) have been found in seedlings of most species of cacti that have fibrous wood in their adult bodies. Consequently, this cell type is now known to be present in almost all cacti. Earlier studies of adult plants revealed WBTs to be present only in cacti with globose or short, broad bodies, whereas all species with large columnar or long slender bodies had fibrous wood without WBTs. However, even these species produce WBTs during the first several months after germination. In species with fibrous wood in their adult bodies (species with large or slender bodies), seedlings undergo a phase transition in wood morphogenesis after a few months and stop producing the juvenile (WBT) wood and begin producing adult (fibrous) wood. If adult plants have an intermediate size, the phase transition is delayed and the plant produces WBT wood for several years. Species with globose bodies repress the phase transition completely and never switch to producing adult (fibrous) wood. Because WBTs are so widespread, they probably originated only once in Cactaceae, not multiple times as suggested earlier, or there may have been just a single origin in the Cactaceae/Portulacaceae clade.

  12. Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae.

    Science.gov (United States)

    Las Peñas, M L; Urdampilleta, J D; Bernardello, G; Forni-Martins, E R

    2009-01-01

    Karyotype analyses in members of the four Cactaceae subfamilies were performed. Numbers and karyotype formula obtained were: Pereskioideae = Pereskiaaculeata(2n = 22; 10 m + 1 sm), Maihuenioideae = Maihuenia patagonica (2n = 22, 9 m + 2 sm; 2n = 44, 18 m + 4 sm), Opuntioideae = Cumulopuntia recurvata(2n = 44; 20 m + 2 sm), Cactoideae = Acanthocalycium spiniflorum (2n = 22; 10 m + 1 sm),Echinopsis tubiflora (2n = 22; 10 m + 1 sm), Trichocereus candicans (2n = 22, 22 m). Chromosomes were small, the average chromosome length was 2.3 mum. Diploid species and the tetraploid C. recurvata had one terminal satellite, whereas the remaining tetraploid species showed four satellited chromosomes. Karyotypes were symmetrical. No CMA(-)/DAPI(+) bands were detected, but CMA(+)/DAPI(-) bands associated with NOR were always found. Pericentromeric heterochromatin was found in C. recurvata, A. spiniflorum, and the tetraploid cytotype of M. patagonica. The locations of the 18S-26S rDNA sites in all species coincided with CMA(+)/DAPI(-) bands; the same occurred with the sizes and numbers of signals for each species. This technique was applied for the first time in metaphase chromosomes in cacti. NOR-bearing pair no.1 may be homeologous in all species examined. In Cactaceae, the 18S-26S loci seem to be highly conserved.

  13. Elementos traqueais de cinco táxons de Cactaceae da caatinga pernambucana, Brasil Traqueary elements of five taxons of Cactaceae of the caatinga from Pernambuco State, Brazil

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    Emília Cristina Pereira de Arruda

    2004-12-01

    Full Text Available Este trabalho traz a caracterização morfológica de elementos de vaso e WBT ("traqueíde com espessamento largo" de raiz e cladódio de cinco táxons de Cactaceae (Harrisia adscendens (Gurke Britton & Rose, Melocactus × horridus Wedermann Notizbl., M. zehntneri (Britton & Rose Luetzelb., Tacinga inamoena (Schumann N.P. Taylor & Stuppy e T. palmadora (Britton & Rose N.P. Taylor & Stuppy ocorrentes em um trecho de caatinga do Estado de Pernambuco, Brasil. Os elementos de vaso observados tanto nas raízes quanto nos cladódios apresentam-se praticamente idênticos entre os táxons estudados, sendo observadas pontoações alternas, opostas e escalariformes, além de placas de perfuração simples transversal e obliqua. As traqueídes (WBT foram registradas apenas nos cladódios de quatro dos táxons estudados, exceto em Harrisia adscendens. As WBTs apresentam espessamento anelar ou helicoidal e o espessamento misto ocorre apenas em Melocactus. Os elementos traqueais foram medidos, mostrando que em Melocactus tanto a raiz como o cladódio, apresentam elementos de vaso longos e quanto as WBT essas são maiores no cladódio de Melocactus.This work brings a morphological characterization of traqueary elements (vessels and tracheids in root and cladode of five taxons of Cactaceae (Harrisia adscendens (Gurke Britton & Rose, Melocactus × horridus Wedermann Notizbl., M. zehntneri (Britton & Rose Luetzelb., Tacinga inamoena (Schumann N.P. Taylor & Stuppy and T. palmadora (Britton & Rose N.P. Taylor & Stuppy occurent in the caatinga of Pernambuco State, Brazil. The vessels elements observed in the roots and cladodes seem almost same among the studied taxons, were observed alternate, opposite and scalariform pitting, and simple perforation plate transversal or oblique. The tracheids (WBT were exclusively noted in the cladodes of four studied taxons, except Harrisia adscendens. The WBTs with annular and helical thickening are present in all taxons and the

  14. Estudo fitoquímico e atividade antimicrobiana de Pilosocereus pachycladus F. RITTER (CACTACEAE)

    OpenAIRE

    Brito Filho, Severino Gonçalves de

    2015-01-01

    O gênero Pilosocereus pertencente à família Cactaceae se destaca por possuir o maior número de espécies, com 35 exemplares distribuídos desde o México até o Paraguai, sendo o Brasil o país com o maior número de espécies distribuídas em diferentes ambientes, incluindo caatingas, restingas e campos rupestres. A espécie Pilosocereus pachycladus F. RITTER, conhecido como "Facheiro", é utilizada pela medicina popular contra inflamação prostática. O estudo fitoquímico pioneiro desta espécie levou a...

  15. Anti-inflammatory activity of copao (Eulychnia acida Phil., Cactaceae) fruits.

    Science.gov (United States)

    Jiménez-Aspee, Felipe; Alberto, Maria Rosa; Quispe, Cristina; Soriano, Maria del Pilar Caramantin; Theoduloz, Cristina; Zampini, Iris Catiana; Isla, Maria Ines; Schmeda-Hirschmann, Guillermo

    2015-06-01

    Copao (Eulychnia acida Phil., Cactaceae) is an endemic species occurring in northern Chile. The edible fruits of this plant are valued for its acidic and refreshing taste. Phenolic-enriched extracts from copao fruit pulp and epicarp, collected in the Elqui and Limari river valleys, were assessed by its in vitro ability to inhibit the pro-inflammatory enzymes lipoxygenase (LOX) and cyclooxygenases (COX-1 and COX-2). At 100 μg/mL, pulp extracts showed better effect towards LOX than epicarp extract, while COX-2 inhibition was observed for both epicarp and pulp samples. In general, the extracts were inactive towards COX-1. A positive correlation was observed between the anti-inflammatory activity and the main phenolic compounds found in this fruit. Copao fruits from the Limari valley, a main place of collection and commercialization, showed major activity, adding evidence on the possible health-beneficial effects of this native Chilean fruit.

  16. Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC (Cactaceae).

    Science.gov (United States)

    Er, Hui Meng; Cheng, En-Hsiang; Radhakrishnan, Ammu Kutty

    2007-09-25

    The anti-proliferative effects of the aqueous and methanol extracts of leaves of Pereskia bleo (Kunth) DC (Cactaceae) against a mouse mammary cancer cell line (4T1) and a normal mouse fibroblast cell line (NIH/3T3) were evaluated under an optimal (in culture medium containing 10% foetal bovine serum (FBS)) and a sub-optimal (in culture medium containing 0.5% FBS) conditions. Under the optimal condition, the aqueous extract showed a significant (pCactaceae) do not have appreciable anti-proliferative effect on the 4T1 and NIH/3T3 cells as the EC(50) values obtained are greater than 50 microg/mL when tested under optimal culture condition. Moreover, the aqueous extract may form mutagenic compound(s) upon the metabolisation by liver enzymes.

  17. Wood Chemical Composition in Species of Cactaceae: The Relationship between Lignification and Stem Morphology

    Science.gov (United States)

    Canché-Escamilla, Gonzalo; Soto-Hernández, Marcos

    2015-01-01

    In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs) and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content) in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous) with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35%) of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level. PMID:25880223

  18. Wood chemical composition in species of Cactaceae: the relationship between lignification and stem morphology.

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    Jorge Reyes-Rivera

    Full Text Available In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35% of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level.

  19. Wood chemical composition in species of Cactaceae: the relationship between lignification and stem morphology.

    Science.gov (United States)

    Reyes-Rivera, Jorge; Canché-Escamilla, Gonzalo; Soto-Hernández, Marcos; Terrazas, Teresa

    2015-01-01

    In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs) and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content) in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous) with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35%) of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level.

  20. Spatial variation in the community of insects associated with the flowers of Pachycereus weberi (Caryophyllales: Cactaceae).

    Science.gov (United States)

    Figueroa-Castro, Dulce María; Valverde, Pedro Luis; Vite, Fernando; Carrillo-Ruiz, Hortensia

    2014-08-01

    The positive relationship between productivity and species diversity is well-known. Insect communities associated with the flowers of Cactaceae species represent an interesting system to explore the productivity-diversity relationship because branches facing the equator receive more photosynthetically active radiation and have higher productivity. Thus, flowers with contrasting orientations within an individual, and even within a single branch, might differ in productivity. Therefore, higher abundance, species richness, and diversity are expected for the insect communities associated with south-facing flowers. This hypothesis was tested in Pachycereus weberi (J.M. Coulter) Backeberg (Cactaceae). Insects within flowers with contrasting orientations were collected and its abundance, richness, and diversity were estimated. We also asked if insects prefer big flowers. Thus, flower volume was estimated and regression analyses were conducted to test if there is a positive relationship between flower size and insect abundance. Flower orientation did not affect species richness. However, species abundance and diversity were different in flowers with contrasting orientations. In general, species abundance was higher in flowers facing southwards than in north-facing flowers. On the contrary, species diversity was higher in north-facing flowers. Abundance of Coleoptera was explained by flower volume in south-facing flowers. Contrary to our hypothesis, total diversity was greater in the less productive oriented flowers. Three possible explanations are discussed to explain the low diversity found in the highly productive, south-facing flowers. Our study provides evidence for the effects of productivity on the structure of insect communities at a very small-scale.

  1. DNA barcodes for Mexican Cactaceae, plants under pressure from wild collecting.

    Science.gov (United States)

    Yesson, Chris; Bárcenas, Rolando T; Hernández, Héctor M; Ruiz-Maqueda, María de la Luz; Prado, Alberto; Rodríguez, Víctor M; Hawkins, Julie A

    2011-09-01

    DNA barcodes could be a useful tool for plant conservation. Of particular importance is the ability to identify unknown plant material, such as from customs seizures of illegally collected specimens. Mexican cacti are an example of a threatened group, under pressure because of wild collection for the xeriscaping trade and private collectors. Mexican cacti also provide a taxonomically and geographically coherent group with which to test DNA barcodes. Here, we sample the matK barcode for 528 species of Cactaceae including approximately 75% of Mexican species and test the utility of the matK region for species-level identification. We find that the matK DNA barcode can be used to identify uniquely 77% of species sampled, and 79-87% of species of particular conservation importance. However, this is far below the desired rate of 95% and there are significant issues for PCR amplification because of the variability of primer sites. Additionally, we test the nuclear ITS regions for the cactus subfamily Opuntioideae and for the genus Ariocarpus (subfamily Cactoideae). We observed higher rates of variation for ITS (86% unique for Opuntioideae sampled) but a much lower PCR success, encountering significant intra-individual polymorphism in Ariocarpus precluding the use of this marker in this taxon. We conclude that the matK region should provide useful information as a DNA barcode for Cactaceae if the problems with primers can be addressed, but matK alone is not sufficiently variable to achieve species-level identification. Additional complementary regions should be investigated as ITS is shown to be unsuitable.

  2. Expression of the KNOTTED HOMEOBOX Genes in the Cactaceae Cambial Zone Suggests Their Involvement in Wood Development.

    Science.gov (United States)

    Reyes-Rivera, Jorge; Rodríguez-Alonso, Gustavo; Petrone, Emilio; Vasco, Alejandra; Vergara-Silva, Francisco; Shishkova, Svetlana; Terrazas, Teresa

    2017-01-01

    The vascular cambium is a lateral meristem that produces secondary xylem (i.e., wood) and phloem. Different Cactaceae species develop different types of secondary xylem; however, little is known about the mechanisms underlying wood formation in the Cactaceae. The KNOTTED HOMEOBOX (KNOX) gene family encodes transcription factors that regulate plant development. The role of class I KNOX genes in the regulation of the shoot apical meristem, inflorescence architecture, and secondary growth is established in a few model species, while the functions of class II KNOX genes are less well understood, although the Arabidopsis thaliana class II KNOX protein KNAT7 is known to regulate secondary cell wall biosynthesis. To explore the involvement of the KNOX genes in the enormous variability of wood in Cactaceae, we identified orthologous genes expressed in species with fibrous (Pereskia lychnidiflora and Pilosocereus alensis), non-fibrous (Ariocarpus retusus), and dimorphic (Ferocactus pilosus) wood. Both class I and class II KNOX genes were expressed in the cactus cambial zone, including one or two class I paralogs of KNAT1, as well as one or two class II paralogs of KNAT3-KNAT4-KNAT5. While the KNOX gene SHOOTMERISTEMLESS (STM) and its ortholog ARK1 are expressed during secondary growth in the Arabidopsis and Populus stem, respectively, we did not find STM orthologs in the Cactaceae cambial zone, which suggests possible differences in the vascular cambium genetic regulatory network in these species. Importantly, while two class II KNOX paralogs from the KNAT7 clade were expressed in the cambial zone of A. retusus and F. pilosus, we did not detect KNAT7 ortholog expression in the cambial zone of P. lychnidiflora. Differences in the transcriptional repressor activity of secondary cell wall biosynthesis by the KNAT7 orthologs could therefore explain the differences in wood development in the cactus species.

  3. Primer reporte del empleo de marcadores ISTR en Cactaceae (Pilosocereus sp

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    Grecia Montalvo Fernández

    2010-10-01

    Full Text Available Título en inglés: First report of the employment of ISTR markers in Cactaceae (Pilosocereus sp Resumen Pilosocereus sp es una especie en peligro crítico de extinción, la única población conocida se encuentra en una mina de mármol verde, hoy abandonada, en la que su explotación produjo la disminución del 80% de la población en 3 años; en la actualidad quedan 28 ejemplares, de ellos unos pocos son adultos, de los cuales solo dos producen frutos. Una de las etapas necesaria para su recuperación es la producción de plántulas para realizar el reforzamiento de la población natural. Como las plantas obtenidas serán plantadas en condiciones naturales, donde se enfrentarán a diversas situaciones ambientales, es conveniente realizar un estudio de diversidad genética. El objetivo de este trabajo fue estimar la variabilidad genética de plántulas de Pilosocereus sp empleando la técnica Inverse Sequence Tagged Repeat (ISTR. Se realizó la germinación in vitro de semillas y se determinó la variabilidad genética de las plántulas obtenidas. Con el análisis molecular se detectaron un total de 97 bandas, de ellas el 62,8% fueron polimórficas. El mayor porcentaje de bandas polimórficas (85,7% se obtuvo con la combinación de oligonucleótidos F6/B6. Con las combinaciones de oligonucleótidos empleados se detectaron de 4 a 6 patrones de banda diferentes. La heterocigosidad media esperada fue de 0,39. Palabras clave: Cactaceae; variabilidad; extinción; polimorfismo. Abstract Pilosocereus sp is a species in critical extinction danger, the only known population is in a mine of green marble, abandoned today, but it exploitation produced the decrease of the population's 80% in 3 years, at the present time they are 28 individuals, of them some few ones are mature, of those which alone two produce fruits. One of the necessary stages for their recovery is the seedlings production to carry out the natural population's reinforcement. As the

  4. Classical macroautophagy in Lobivia rauschii (Cactaceae) and possible plastidial autophagy in Tillandsia albida (Bromeliaceae) tapetum cells.

    Science.gov (United States)

    Papini, Alessio; Mosti, Stefano; van Doorn, Wouter G

    2014-05-01

    The tapetum in anthers is a tissue that undergoes programmed cell death (PCD) during the production of pollen. We observed two types of autophagy prior to cell death. In Lobivia rauschii (Cactaceae), tapetum cells showed plant-type autophagosomes-autolysosomes, which have been found previously exclusively in root meristem cells. The autophagic structures were formed by a network of tubules which apparently merged laterally, thereby sequestering a portion of the cytoplasm. The organelles observed in the sequestered material included multilamellar bodies, which have not been reported earlier in these organelles. By contrast, Tillandsia albida (Bromeliaceae) tapetum cells contained no such organelles but showed plastids that might possibly carry out autophagy, as they contained portions of the cytoplasm similar to the phenomenon reported earlier in Phaseolus and Dendrobium. However, the ultrastructure of the T. albida plastids was different from that in the previous reports. It is concluded that in L. rauschii classical plant macroautophagy was involved in degradation of the cytoplasm, while in T. albida such classical macroautophagy was not observed. Instead, the data in T. albida suggested the hypothesis that plastids are able to carry out degradation of the cytoplasm.

  5. Phylogenetic relationships in Peniocereus (Cactaceae) inferred from plastid DNA sequence data.

    Science.gov (United States)

    Arias, Salvador; Terrazas, Teresa; Arreola-Nava, Hilda J; Vázquez-Sánchez, Monserrat; Cameron, Kenneth M

    2005-10-01

    The phylogenetic relationships of Peniocereus (Cactaceae) species were studied using parsimony analyses of DNA sequence data. The plastid rpl16 and trnL-F regions were sequenced for 98 taxa including 17 species of Peniocereus, representatives from all genera of tribe Pachycereeae, four genera of tribe Hylocereeae, as well as from three additional outgroup genera of tribes Calymmantheae, Notocacteae, and Trichocereeae. Phylogenetic analyses support neither the monophyly of Peniocereus as currently circumscribed, nor the monophyly of tribe Pachycereeae since species of Peniocereus subgenus Pseudoacanthocereus are embedded within tribe Hylocereeae. Furthermore, these results show that the eight species of Peniocereus subgenus Peniocereus (Peniocereus sensu stricto) form a well-supported clade within subtribe Pachycereinae; P. serpentinus is also a member of this subtribe, but is sister to Bergerocactus. Moreover, Nyctocereus should be resurrected as a monotypic genus. Species of Peniocereus subgenus Pseudoacanthocereus are positioned among species of Acanthocereus within tribe Hylocereeae, indicating that they may be better classified within that genus. A number of morphological and anatomical characters, especially related to the presence or absence of dimorphic branches, are discussed to support these relationships.

  6. Complete nucleotide sequence and genome organization of a Cactus virus X strain from Hylocereus undatus (Cactaceae).

    Science.gov (United States)

    Liou, M R; Chen, Y R; Liou, R F

    2004-05-01

    The complete nucleotide sequence of a strain of Cactus virus X (CVX-Hu) isolated from Hylocereus undatus (Cactaceae) has been determined. Excluding the poly(A) tail, the sequence is 6614 nucleotides in length and contains seven open reading frames (ORFs). The genome organization of CVX is similar to that of other potexviruses. ORF1 encodes the putative viral replicase with conserved methyltransferase, helicase, and polymerase motifs. Within ORF1, two other ORFs were located separately in the +2 reading frame, we call these ORF6 and ORF7. ORF2, 3, and 4, which form the "triple gene block" characteristic of the potexviruses, encode proteins with molecular mass of 25, 12, and 7 KDa, respectively. ORF5 encodes the coat protein with an estimated molecular mass of 24 KDa. Sequence analysis indicated that proteins encoded by ORF1-5 display certain degree of homology to the corresponding proteins of other potexviruses. Putative product of ORF6, however, shows no significant similarity to those of other potexviruses. Phylogenetic analyses based on the replicase (the methyltransferase, helicase, and polymerase domains) and coat protein demonstrated a closer relationship of CVX with Bamboo mosaic virus, Cassava common mosaic virus, Foxtail mosaic virus, Papaya mosaic virus, and Plantago asiatica mosaic virus.

  7. Direct anthelmintic effects of Cereus jamacaru (Cactaceae) on trichostrongylid nematodes of sheep: in vivo studies.

    Science.gov (United States)

    Vatta, A F; Kandu-Lelo, C; Ademola, I O; Eloff, J N

    2011-08-25

    Following claims of anthelmintic activity of Cereus jamacaru DC (Cactaceae) by a commercial farmer, in vivo studies were conducted to determine the possible direct anthelmintic effects of the plant on ovine gastrointestinal nematodes. Eighteen sheep were infected with 4000 Haemonchus contortus and 6000 Trichostrongylus colubriformis larvae given in three divided doses over a period of three days. Once the infections were patent, the sheep were allocated to three groups and were drenched once a week for six weeks with fresh blended C. jamacaru plant material at a single (32.3g/sheep) or double dose (64.6g/sheep) or they remained as undrenched controls. Faeces were collected from individual animals on the day of treatment and three days thereafter on a weekly basis for seven weeks for faecal egg count. While there were no statistically significant differences in the egg counts between the groups, a double dose of C. jamacaru was effective in reducing the egg counts in the sheep by 18-65% over the 49 days of the experiment. Given that all animals remained in good health throughout the course of the experiment, with no adverse events occurring during the study, further experiments using higher doses or administering the plant material for a longer period of time than in the present study would be warranted.

  8. Correlated evolution of stem and leaf hydraulic traits in Pereskia (Cactaceae).

    Science.gov (United States)

    Edwards, Erika J

    2006-01-01

    Recent studies have demonstrated significant correlations between stem and leaf hydraulic properties when comparing across species within ecological communities. This implies that these traits are co-evolving, but there have been few studies addressing plant water relations within an explicitly evolutionary framework. This study tests for correlated evolution among a suite of plant water-use traits and environmental parameters in seven species of Pereskia (Cactaceae), using phylogenetically independent contrasts. There were significant evolutionary correlations between leaf-specific xylem hydraulic conductivity, Huber Value, leaf stomatal pore index, leaf venation density and leaf size, but none of these traits appeared to be correlated with environmental water availability; only two water relations traits - mid-day leaf water potentials and photosynthetic water use efficiency - correlated with estimates of moisture regime. In Pereskia, it appears that many stem and leaf hydraulic properties thought to be critical to whole-plant water use have not evolved in response to habitat shifts in water availability. This may be because of the extremely conservative stomatal behavior and particular rooting strategy demonstrated by all Pereskia species investigated. These results highlight the need for a lineage-based approach to understand the relative roles of functional traits in ecological adaptation.

  9. Studies on betaxanthin profiles of vegetables and fruits from the Chenopodiaceae and Cactaceae.

    Science.gov (United States)

    Kugler, Florian; Graneis, Stephan; Stintzing, Florian C; Carle, Reinhold

    2007-01-01

    The present study provides an update on the betaxanthin (bx) compositions of red and yellow beetroots, yellow-coloured Swiss chard petioles, and yellow-orange cactus pear. Applying RP-HPLC coupled with positive ion electrospray mass spectrometry and by comparison with UV-vis and mass spectrometric characteristics as well as retention times of semi-synthesized reference compounds, 24 betaxanthins were identified in red and yellow beetroot hypocotyls. Twenty-five and thirteen betaxanthins were present in yellow Swiss chard petioles and the cactus pear cultivar 'Gialla', respectively. Ethanolamine-bx and threonine-bx were found to be novel betaxanthins in Chenopodiaceae representatives, which to the best of our knowledge have not been reported as genuine pigments so far. Furthermore, aspartic acid-bx (miraxanthin II), lysine-bx, and methionine-bx, hitherto found in other families, were identified in the Chenopodiaceae for the first time. Additionally, tyrosine-bx (portulacaxanthin II) and tryptophan-bx have not been earlier reported to occur in the Cactaceae. These findings provide valuable phytochemical information and may be useful for a better understanding of the functional properties of betaxanthins in plants.

  10. Molecular phylogeny and character evolution in terete-stemmed Andean opuntias (Cactaceae-Opuntioideae).

    Science.gov (United States)

    Ritz, C M; Reiker, J; Charles, G; Hoxey, P; Hunt, D; Lowry, M; Stuppy, W; Taylor, N

    2012-11-01

    The cacti of tribe Tephrocacteae (Cactaceae-Opuntioideae) are adapted to diverse climatic conditions over a wide area of the southern Andes and adjacent lowlands. They exhibit a range of life forms from geophytes and cushion-plants to dwarf shrubs, shrubs or small trees. To confirm or challenge previous morphology-based classifications and molecular phylogenies, we sampled DNA sequences from the chloroplast trnK/matK region and the nuclear low copy gene phyC and compared the resulting phylogenies with previous data gathered from nuclear ribosomal DNA sequences. The here presented chloroplast and nuclear low copy gene phylogenies were mutually congruent and broadly coincident with the classification based on gross morphology and seed micro-morphology and anatomy. Reconstruction of hypothetical ancestral character states suggested that geophytes and cushion-forming species probably evolved several times from dwarf shrubby precursors. We also traced an increase of embryo size at the expense of the nucellus-derived storage tissue during the evolution of the Tephrocacteae, which is thought to be an evolutionary advantage because nutrients are then more rapidly accessible for the germinating embryo. In contrast to these highly concordant phylogenies, nuclear ribosomal DNA data sampled by a previous study yielded conflicting phylogenetic signals. Secondary structure predictions of ribosomal transcribed spacers suggested that this phylogeny is strongly influenced by the inclusion of paralogous sequence probably arisen by genome duplication during the evolution of this plant group.

  11. [Triatominae and Cactaceae: a risk for the transmission of the American trypanosomiasis in the peridomicilary space (Northeast Brazil)].

    Science.gov (United States)

    Emperaire, L; Romaña, C A

    2006-06-01

    Field observations carried in semi-arid Brazil Northeast point out the frequent association, in the peridomiciliary space, between a cactus, Cereus jamacaru, the occurrence of nests in its branches and the occurrence of two species of insects vectors of Trypanosoma cruzi, pathogenic agent of Chagas disease: Rhodnius neglectus and Triatoma pseudomaculata. The analysis of the architectural variables of this Cactaceae shows that the presence of nests, and thus of insects, depends on the traditional practices of management of this cactus. This study underlines the relevance of an integrated approach of the ecology of Triatominae for the identification of factors of risk.

  12. A new Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis (Cactaceae).

    Science.gov (United States)

    Triapitsyn, Serguei V; Aguirre, María B; Logarzo, Guillermo A

    2016-05-26

    A new species of Anagyrus Howard (Hymenoptera: Encyrtidae), A. lapachosus sp. n., is described from Salta Province of Argentina as a parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis cactus (Cactaceae). It is a candidate "new association" biological control agent for quarantine evaluation and possible following introduction to Puerto Rico (USA) against another Hypogeococcus sp., commonly called the Harrisia cactus mealybug and often misidentified as H. pungens Granara de Willink (according to our unpublished data the latter attacks only Amaranthaceae), which devastates or threatens the native cacti there and also in some other Caribbean islands (Triapitsyn, Aguirre et al. 2014; Carrera-Martínez et al. 2015).

  13. Studies on the Biology of Hypogeococcus pungens (sensu stricto) (Hemiptera: Pseudococcidae) in Argentina to Aid the Identification of the Mealybug Pest of Cactaceae in Puerto Rico.

    Science.gov (United States)

    Aguirre, M B; Diaz-Soltero, H; Claps, L E; Saracho Bottero, A; Triapitsyn, S; Hasson, E; Logarzo, G A

    2016-01-01

    Hypogeococcus pungens Granara de Willink, sensu stricto, is a serious pest of cacti in Puerto Rico threating many Caribbean islands. A classical biological control program for H. pungens was initiated for Puerto Rico in 2010 with a survey for natural enemies of H. pungens in its native range of Argentina. Biological differences were observed between populations of H. pungens sampled on Amaranthaceae and Cactaceae. Molecular studies suggested that H. pungens populations from different host plant families are likely a complex of species. Our objective was to study the biology of H. pungens sensu stricto on specimens collected in the same locality and host plant as the holotype [Tucumán Province, Argentina; Alternanthera pungens Kunth (Amaranthaceae)]. We were interested in the reproductive biology of females, longevity and survival of adults, the effect of temperature on the development, and nymph performance (survival and development) on five Cactaceae species. We found that H. pungens s.s showed marked biological differences from the populations collected on Cactaceae and exported to Australia for the biological control of the cactus Harrisia spp. The main differences were the presence of deuterotoky parthenogenesis and the fact that H. pungens did not attack Cactaceae in the laboratory. Our results provide biological evidence that H. pungens is a species complex. We propose that the population introduced to Australia is neither Hypogeococcus festerianus Lizer y Trelles nor H. pungens, but an undescribed species with three circuli, and that the Hypogeococcus pest of cacti in Puerto Rico is not H. pungens.

  14. Studies on the Biology of Hypogeococcus pungens (sensu stricto) (Hemiptera: Pseudococcidae) in Argentina to Aid the Identification of the Mealybug Pest of Cactaceae in Puerto Rico

    Science.gov (United States)

    Aguirre, M. B.; Diaz-Soltero, H.; Claps, L. E.; Saracho Bottero, A.; Triapitsyn, S.; Hasson, E.; Logarzo, G. A.

    2016-01-01

    Hypogeococcus pungens Granara de Willink, sensu stricto, is a serious pest of cacti in Puerto Rico threating many Caribbean islands. A classical biological control program for H. pungens was initiated for Puerto Rico in 2010 with a survey for natural enemies of H. pungens in its native range of Argentina. Biological differences were observed between populations of H. pungens sampled on Amaranthaceae and Cactaceae. Molecular studies suggested that H. pungens populations from different host plant families are likely a complex of species. Our objective was to study the biology of H. pungens sensu stricto on specimens collected in the same locality and host plant as the holotype [Tucumán Province, Argentina; Alternanthera pungens Kunth (Amaranthaceae)]. We were interested in the reproductive biology of females, longevity and survival of adults, the effect of temperature on the development, and nymph performance (survival and development) on five Cactaceae species. We found that H. pungens s.s. showed marked biological differences from the populations collected on Cactaceae and exported to Australia for the biological control of the cactus Harrisia spp. The main differences were the presence of deuterotoky parthenogenesis and the fact that H. pungens did not attack Cactaceae in the laboratory. Our results provide biological evidence that H. pungens is a species complex. We propose that the population introduced to Australia is neither Hypogeococcus festerianus Lizer y Trelles nor H. pungens, but an undescribed species with three circuli, and that the Hypogeococcus pest of cacti in Puerto Rico is not H. pungens. PMID:27324585

  15. Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study.

    Science.gov (United States)

    Badalamenti, Ornella; Carra, Angela; Oddo, Elisabetta; Carimi, Francesco; Sajeva, Maurizio

    2016-01-01

    Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Ehrenberg, an Appendix I CITES listed species critically endangered and sought after by the horticultural trade, on micropropagated Opuntia ficus-indica Miller. Apical and sub-apical scions of P. aselliformis were used to perform micrografting with a successful rate of 97 and 81 % respectively. Survival rate after ex vivo transfer was 85 %. We hypothesize that this method could be applied to other endangered, slow growing taxa of Cactaceae thus contributing to the conservation of this endangered family.

  16. Estudo anatômico de folha e caule de Pereskia aculeata Mill. (Cactaceae

    Directory of Open Access Journals (Sweden)

    M.R. Duarte

    2005-06-01

    Full Text Available Pereskia aculeata Mill. (Cactaceae é uma trepadeira arbustiva conhecida comumente como ora-pro-nobis. Apresenta elevado teor de mucilagem e proteína, sendo empregada na indústria alimentícia e farmacêutica. Na medicina popular, é utilizada externamente como emoliente. Este trabalho teve por objetivo estudar a anatomia foliar e caulinar da planta, a fim de fornecer informações complementares à sua identificação e aplicáveis ao seu controle de qualidade. Folhas adultas e fragmentos de caules foram fixados, seccionados à mão livre e corados com azul de astra e fucsina básica ou com azul de toluidina. Testes histoquímicos e análise ultra-estrutural de superfície foram realizados. A folha é simples e elíptica. Possui epiderme uniestratificada, estômatos paracíticos em ambas as faces, mesofilo que tende a ser dorsiventral, contendo numerosas drusas de oxalato de cálcio e células isodiamétricas grandes com conteúdo mucilaginoso. A nervura central é percorrida por um feixe vascular colateral em arco aberto. O pecíolo tem secção plano-convexa e um feixe colateral em formato de arco fechado. O caule, em estrutura secundária incipiente, possui epiderme unisseriada, colênquima angular, parênquima cortical com muitos amiloplastos, calotas de fibras perivasculares e organização vascular colateral. No córtex e na medula, são observadas numerosas cavidades com conteúdo mucilaginoso e drusas de oxalato de cálcio, estas também presentes no floema.

  17. Vivipary and offspring survival in the epiphytic cactus Epiphyllum phyllanthus (Cactaceae).

    Science.gov (United States)

    Cota-Sánchez, J Hugo; Abreu, Deusa D

    2007-01-01

    Vivipary, the germination of seeds before they are shed from the parent plant, is a rare event in angiosperms involving complex ecophysiological processes. Pseudovivipary and cryptovivipary occur in approximately 30 (2%) species of the cactus family. A remarkable case of vivipary in Epiphyllum phyllanthus is described here. Information is provided regarding the biology of viviparous fruits, morphology, mortality, survival rates of viviparous offspring, and some eco-evolutionary implications of this reproductive strategy in the Cactaceae. This epiphytic cactus has no host-specific relationship. A low proportion (33.3%) of individuals produced viviparous fruits. Seed number/fruit varied from 197 to 230 with percentage of viviparous germination from 97.5% to 99%. The viviparous seedlings exhibited normal development and were no different from non-viviparous offspring. Transplanting experiments showed that the first week is critical for seedling establishment, and high mortality occurred in the three treatments used: 69% on the phorophyte surface, 58.6% on the ground, and 44.8% under controlled conditions. The number of survivors gradually stabilized, and the contribution to establishment was comparable in each of the treatments after the acclimation phase. It is suggested that vivipary is associated with thermoregulation, parental care, conspecific nursing, and rapid seedling establishment. Germination is not a limiting factor in the perpetuation of this viviparous species, but seedling establishment is. In viviparous individuals of E. phyllanthus, seedling mortality during establishment rather than failure to germinate within the fruit is a limiting factor affecting local population density. Overall, viviparity is an intrinsic, genetic event involving high metabolic costs favouring germination and dispersal of the fittest offspring regardless of substrate and environmental conditions.

  18. Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae.

    Science.gov (United States)

    Koenig, R; Pleij, C W A; Loss, S; Burgermeister, W; Aust, H; Schiemann, J

    2004-05-01

    The genome properties of three potexviruses which previously had been isolated from different genera in the family Cactaceae and had been found to be only distantly related serologically have been studied. The sequence of the 3040 3' terminal nucleotides of the genomic RNA of isolate K11 from Schlumbergera bridgesii and the complete RNA sequences of isolates B1 and CC10 from Zygocactus sp. and Opuntia sp., respectively, were determined. Starting sequences were obtained by means of immunocapture reverse transcription PCR using primers derived from highly conserved sequences in other potexviral RNAs. The known parts of the sequences were extended by means of random-primed cDNAs and specific primers derived from the known parts of the sequences. The genome structure of the three viruses resembles that of other potexviruses. The conserved motifs typical for replication-associated proteins, triple gene block (TGB) proteins and coat proteins of potexviruses were readily identified in the translation products of the five open reading frames. The 3' untranslated regions of the three RNAs are folded into secondary structures containing three characteristic hairpins. Rather low percentages of amino acid sequence identities ranging from 62% to 76% for the coat proteins and 41% to 49% for TGB proteins 3 suggest that these viruses should be regarded as distinct virus species for which the names Zygocactus virus X, Schlumbergera virus X and Opuntia virus X are proposed. It is also suggested that the name Cactus virus X which originally was coined for all three virus isolates should no longer be used.

  19. Spine micromorphology of normal and hyperhydric Mammillaria gracilis Pfeiff. (Cactaceae) shoots.

    Science.gov (United States)

    Peharec, P; Posilović, H; Balen, B; Krsnik-Rasol, M

    2010-07-01

    Artificial conditions of tissue culture affect growth and physiology of crassulacean acid metabolism plants which often results in formation of hyperhydric shoots. In in vitro conditions Mammillaria gracilis Pfeiff. (Cactaceae) growth switches from organized to unorganized way, producing a habituated organogenic callus which simultaneously regenerates morphologically normal as well as altered hyperhydric shoots. In this study, influence of tissue culture conditions on morphology of cactus spines of normal and hyperhydric shoots was investigated. Spines of pot-grown Mammillaria plants and of in vitro regenerated shoots were examined with stereo microscope and scanning electron microscope. The pot-grown plants had 16-17 spines per areole. In vitro grown normal shoots, even though they kept typical shoot morphology, had lower number of spines (11-12) and altered spine morphology. This difference was even more pronounced in spine number (six to seven) and morphology of the hyperhydric shoots. Scanning electron microscopy analysis revealed remarkable differences in micromorphology of spine surface between pot-grown and in vitro grown shoots. Spines of in vitro grown normal shoots showed numerous long trichomes, which were more elongated on spines of the hyperhydric shoots; the corresponding structures on spine surface of pot-grown plants were noticed only as small protrusions. Scanning electron microscopy morphometric studies showed that the spines of pot-grown plants were significantly longer compared to the spines of shoots grown in tissue culture. Moreover, transverse section shape varies from elliptical in pot-grown plants to circular in normal and hyperhydric shoots grown in vitro. Cluster and correspondence analyses performed on the scanning electron microscope obtained results suggest great variability among spines of pot-grown plants. Spines of in vitro grown normal and hyperhydric shoots showed low level of morphological variation among themselves despite the

  20. Palinología de los géneros de Cactaceae Maihuenia (Maihuenioideae y Pereskia (Pereskioideae de Argentina

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    Silvina S. Garralla

    2009-07-01

    Full Text Available Pollen grains of five species from two genera of the subfamilies Maihuenioideae and Pereskioideae (Cactaceae are described with the optical and scanning microscopes. Maihuenia (F.A.C.Weber K.Schum.: M. patagonica (Phil. Britton & Rose and M. poeppigii (Otto ex Pfeiff. F.A.C. Weber ex K. Schum. (Maihuenioideae and Pereskia Mill.: P. aculeata Mill., P. nemorosa Rojas Acosta and P. sacharosa Griseb. (Pereskioideae. The studied pollen grains of genera Maihuenia are prolate or spheroidal, medium or large sized, and Pereskia are all spheroidal and large sized. The apertures show variation in number: three to fourteen, colpate, with different pattern distribution and length of the colpus: Maihuenia has tricolpate grains and Pereskia 6-14 pantocolpate, some of them form syncolpos. The sculpture does not show marked differences between the species, which is microechinate and nanoechinate with perforations surrounded by a thickening. A key to determine the species of each genus is presented.

  1. Estudios palinológicos de especies argentinas de los géneros Cereus, Cleistocactus, Denmoza, Echinopsis y Monvillea (Cactaceae, Cactoideae Palynological studies in Argentina species of the genera Cereus, Cleistocactus, Denmoza, Echinopsis and Monvillea (Cactaceae, Cactoideae

    Directory of Open Access Journals (Sweden)

    Elsa Lattar

    2010-06-01

    Full Text Available Se estudió la morfología del polen de 19 especies pertenecientes a cinco géneros (Cereus, Cleistocactus, Denmoza, Echinopsis y Monvillea de la subfamilia Cactoideae (Cactaceae en Argentina, fueron descriptas usando microscopio óptico y microscopio electrónico de barrido: Cereus aethiops Haw., C. argentinensis Britton & Rose, C. forbesii Otto ex C.F.Först., C. haenkeanus F.A.C. Weber ex K. Schum., C. stenogonus K. Schum., C. uruguayanus R. Kiesling; Cleistocactus baumannii (Lem. Lem., C. hyalacanthus (K. Schum. Gosselin, C. smaragdiflorus (F.A.C. Weber Britton & Rose; Denmoza rhodacantha (Salm-Dyck Britton & Rose; Echinopsis ancistrophora Speg., E. aurea Britton & Rose, E. leucantha (Gillies ex Salm-Dyck Walp., E. mamillosa Gürke, E. oxygona (Lynk Zucc., E. rhodotricha K. Schum., E. tubiflora (Pfeiff. & Zucc., Monvillea cavendischii (Monv. Britton & Rose y M. spegazzinii (F.A.C. Weber Britton & Rose. Los granos de polen de las especies analizadas son estenopalínicos, principalmente tricolpados, subesferoidales a prolatos, con téctum microperforado, espinulado, microespinulado o nanoespinulado. Encontrándose pequenas diferencias en forma y tamano. Se determinaron dos tipos polínicos: Polen Tipo Cereus argentinensis y Polen Tipo Echinopsis mamillosa. Se presenta una clave para identificar y distinguir los tipos citados.Pollen grains morphology of nineteen species belonging to five genera (Cereus, Cleistocactus, Denmoza, Echinopsis and Monvillea of the subfamily Cactoideae (Cactaceae in Argentina, are described using optical and scanning electron microscopy: Cereus aethiops Haw., C. argentinensis Britton & Rose, C. forbesii Otto ex C.F.Först., C. haenkeanus F.A.C. Weber ex K. Schum., C. stenogonus K. Schum., C. uruguayanus R. Kiesling; Cleistocactus baumannii (Lem. Lem., C. hyalacanthus (K. Schum. Gosselin, C. smaragdiflorus (F.A.C. Weber Britton & Rose; Denmoza rhodacantha (Salm-Dyck Britton & Rose; Echinopsis ancistrophora Speg., E

  2. Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line.

    Science.gov (United States)

    Tan, M L; Sulaiman, S F; Najimuddin, N; Samian, M R; Muhammad, T S Tengku

    2005-01-04

    Currently, breast cancer is the leading cause of cancer-related death in women. Therefore, there is an urgent need to develop alternative therapeutic measures against this deadly disease. Here, we report the cytotoxicity activity and the mechanism of cell death exhibited by the methanol extract prepared from Pereskia bleo (Kunth) DC. (Cactaceae) plant against human breast carcinoma cell line, T-47D. In vitro cytotoxicity screening of methanol extract of Pereskia bleo plant indicated the presence of cytotoxicity activity of the extract against T-47D cells with EC50 of 2.0 microg/ml. T-47D cell death elicited by the extract was found to be apoptotic in nature based a clear indication of DNA fragmentation which is a hallmark of apoptosis. In addition, ultrastructural analysis also revealed apoptotic characteristics (the presence of chromatin margination and apoptotic bodies) in the extract-treated cells. RT-PCR analysis showed the mRNA expression levels of c-myc, and caspase 3 were markedly increased in the cells treated with the plant extract. However, p53 expression was only slightly increased as compared to caspase 3 and c-myc. Thus, the results from this study strongly suggest that the methanol extract of Pereskia bleo may contain bioactive compound(s) that caused breast carcinoma, T-47D cell death by apoptosis mechanism via the activation of caspase-3 and c-myc pathways.

  3. New Alcamide and Anti-oxidant Activity of Pilosocereus gounellei A. Weber ex K. Schum. Bly. ex Rowl. (Cactaceae

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    Jéssica K. S. Maciel

    2015-12-01

    Full Text Available The Cactaceae family is composed by 124 genera and about 1438 species. Pilosocereus gounellei, popularly known in Brazil as xique-xique, is used in folk medicine to treat prostate inflammation, gastrointestinal and urinary diseases. The pioneering phytochemical study of P. gounellei was performed using column chromatography and HPLC, resulting in the isolation of 10 substances: pinostrobin (1, β-sitosterol (2, a mixture of sitosterol 3-O-β-d-glucopyranoside/stigmasterol 3-O-β-d-glucopyranoside (3a/3b, 132-hydroxyphaeophytin a (4, phaeophytin a (5, a mixture of β-sitosterol and stigmasterol (6a/6b, kaempferol (7, quercetin (8, 7′-ethoxy-trans-feruloyltyramine (mariannein, 9 and trans-feruloyl tyramine (10. Compound 9 is reported for the first time in the literature. The structural characterization of the compounds was performed by analyses of 1-D and 2-D NMR data. In addition, a phenolic and flavonol total content assay was carried out, and the anti-oxidant potential of P. gounellei was demonstrated.

  4. Influence of Pleistocene glacial/interglacial cycles on the genetic structure of the mistletoe cactus Rhipsalis baccifera (Cactaceae) in Mesoamerica.

    Science.gov (United States)

    Ornelas, Juan Francisco; Rodríguez-Gómez, Flor

    2015-01-01

    Phylogeographical work on cloud forest-adapted species provides inconsistent evidence on cloud forest dynamics during glacial cycles. A study of Rhipsalis baccifera (Cactaceae), a bird-dispersed epiphytic mistletoe cactus, was conducted to investigate genetic variation at sequence data from nuclear [internal transcribed spacer (ITS), 677 bp] and chloroplast (rpl32-trnL, 1092bp) DNA for 154 individuals across the species range in Mesoamerica to determine if such patterns are consistent with the expansion/contraction model of cloud forest during glacial cycles. We conducted population and spatial genetic analyses as well as gene flow and divergence time estimates between 24 populations comprising the distribution of R. baccifera in Mexico and Guatemala to gain insight of the evolutionary history of these populations, and a complementary species distribution modeling approach to frame information derived from the genetic analyses into an explicit paleoecological context. The results revealed a phylogeographical break at the Isthmus of Tehuantepec, and high levels of genetic diversity among populations and cloud forest areas. Despite the genetic differentiation of some R. baccifera populations, the widespread ITS ribotypes suggest effective nuclear gene flow via pollen and population differentiation shown by the rpl32-trnL suggests more restricted seed flow. Predictions of species distribution models under past last glacial maximum (LGM) climatic conditions and a significant signal of demographic expansion suggest that R. baccifera populations experienced a range expansion tracking the conditions of the cloud forest distribution and shifted to the lowlands with population connectivity during the LGM.

  5. Apical meristem organization and lack of establishment of the quiescent center in Cactaceae roots with determinate growth.

    Science.gov (United States)

    Rodríguez-Rodríguez, José Fernando; Shishkova, Svetlana; Napsucialy-Mendivil, Selene; Dubrovsky, Joseph G

    2003-10-01

    Some species of Cactaceae from the Sonoran Desert are characterized by a determinate growth pattern of the primary root, which is important for rapid lateral-root formation and seedling establishment. An analysis of the determinate root growth can be helpful for understanding the mechanism of meristem maintenance in plants in general. Stenocereus gummosus (Engelm.) Gibson & Horak and Pachycereus pringlei (S. Watson) Britton & Rose are characterized by an open type of root apical meristem. Immunohistochemical analysis of 5-bromo-2'-deoxyuridine incorporation into S. gummosus showed that the percentage of cells passing through the S-phase in a 24-h period is the same within the zone where a population of relatively slowly proliferating cells could be established and above this zone in the meristem. This indicated the absence of the quiescent center (QC) in S. gummosus. During the second and the third days of growth, in the distal meristem portion of P. pringlei roots, a compact group of cells that had a cell cycle longer than in the proximal meristem was found, indicating the presence of the QC. However, later in development, the QC could not be detected in this species. These data suggest that during post-germination the absence of the establishment of the QC within the apical meristem and limited proliferative activity of initial cells are the main components of a determinate developmental program and that establishment of the QC is required for maintenance of the meristem and indeterminate root growth in plants.

  6. New Alcamide and Anti-oxidant Activity of Pilosocereus gounellei A. Weber ex K. Schum. Bly. ex Rowl. (Cactaceae).

    Science.gov (United States)

    Maciel, Jéssica K S; Chaves, Otemberg S; Brito Filho, Severino G; Teles, Yanna C F; Fernandes, Marianne G; Assis, Temilce S; Fernandes, Pedro Dantas; de Andrade, Alberício Pereira; Felix, Leonardo P; Silva, Tania M S; Ramos, Nathalia S M; Silva, Girliane R; de Souza, Maria de Fátima Vanderlei

    2015-12-22

    The Cactaceae family is composed by 124 genera and about 1438 species. Pilosocereus gounellei, popularly known in Brazil as xique-xique, is used in folk medicine to treat prostate inflammation, gastrointestinal and urinary diseases. The pioneering phytochemical study of P. gounellei was performed using column chromatography and HPLC, resulting in the isolation of 10 substances: pinostrobin (1), β-sitosterol (2), a mixture of sitosterol 3-O-β-d-glucopyranoside/stigmasterol 3-O-β-d-glucopyranoside (3a/3b), 13²-hydroxyphaeophytin a (4), phaeophytin a (5), a mixture of β-sitosterol and stigmasterol (6a/6b), kaempferol (7), quercetin (8), 7'-ethoxy-trans-feruloyltyramine (mariannein, 9) and trans-feruloyl tyramine (10). Compound 9 is reported for the first time in the literature. The structural characterization of the compounds was performed by analyses of 1-D and 2-D NMR data. In addition, a phenolic and flavonol total content assay was carried out, and the anti-oxidant potential of P. gounellei was demonstrated.

  7. A mass collection of Triatoma ryckmani (Hemiptera: Reduviidae) from Stenocereus Eichlamii (Cactaceae) in the semiarid region of Guatemala.

    Science.gov (United States)

    Marroquín, Ricardo; Bor, Silvia; Monroy, M Carlota

    2004-12-01

    A population of 216 specimens of Stenocereus eichlamii (Cactaceae, Subfamily Cereoideae) was surveyed for Triatoma ryckmani (vector of Chagas disease) in a one hectare plot of semiarid habitat in Guatemala. Out of 44 plants that had dead and dry sections, 24 plants had a total of 103 specimens of T. ryckmani. In comparison with other areas of Guatemala, T ryckmani is well established in the semiarid ecosystem (Infestation index 54.5, density = 2.3 and crowding index 4.3). The insects were mainly found (52.4%) in the dead portions of S. eichlamii 2.0 to 3.2 m above the ground (X2 = 26.0, P < 0.00001), followed by dry cactus sections between 3.2 and 5.0 m (35.9%). They were less frequent 0.2 to 2.0 m above the ground. A considerable proportion (75.7%) had no aparent blood in their digestive systems. To determine the presence of flagellates, 43 of the bugs were dissected, but none were found. This is the first report on T. ryckmani population dynamics in this habitat.

  8. Populational characterization of Melocactus zehntneri (Britton & Rose Luetzelburg (Cactaceae present in an inselberg of the Paraiban Caatinga

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    Juliano Ricardo Fabricante

    2010-03-01

    Full Text Available Inselbergs are rocky outcrops that are characterized by the fact that they form isolated ecosystems with a high degree of endemism. However, these environments remain little studied. The present work aimed to characterize a population of Melocactus zehntneri (Britton & Rose Luetzelburg (Cactaceae occurring on an inselberg in the municipal district of Pocinhos, PB. Over the whole extension of the inselberg, 50 plots of 1m² were allocated. The sampled individuals were classified according to ontogenetic stadium, evaluating the number of individuals, the height, the diameter in the central section, and the number of dead. To evaluate the pattern of space distribution of the species, the Index of Dispersion of Morisita (Id was calculated, and to verify the influence of one ontogenetic stadium on another, the coefficient of linear correlation was applied (ρ. A total of 869 individuals were sampled, of which 635 were young and 234 were adults. The value of Id was 1.28, indicating that the population distribution was clumped. The value of ρ = -0.3872 demonstrated a negative linear correlation among the ontogenetic stadiums. The accumulation of rocky sediment on some points of the inselberg certainly favored the distribution of the species on it. The population demonstrated stability, with a great number of stock individuals.

  9. Novel seed coat lignins in the Cactaceae: structure, distribution and implications for the evolution of lignin diversity.

    Science.gov (United States)

    Chen, Fang; Tobimatsu, Yuki; Jackson, Lisa; Nakashima, Jin; Ralph, John; Dixon, Richard A

    2013-01-01

    We have recently described a hitherto unsuspected catechyl lignin polymer (C-lignin) in the seed coats of Vanilla orchid and in cacti of one genus, Melocactus (Chen et al., Proc. Natl. Acad. Sci. USA. 2012, 109, 1772-1777.). We have now determined the lignin types in the seed coats of 130 different cactus species. Lignin in the vegetative tissues of cacti is of the normal guaiacyl/syringyl (G/S) type, but members of most genera within the subfamily Cactoidae possess seed coat lignin of the novel C-type only, which we show is a homopolymer formed by endwise β-O-4-coupling of caffeyl alcohol monomers onto the growing polymer resulting in benzodioxane units. However, the species examined within the genera Coryphantha, Cumarinia, Escobaria and Mammillaria (Cactoideae) mostly had normal G/S lignin in their seeds, as did all six species in the subfamily Opuntioidae that were examined. Seed coat lignin composition is still evolving in the Cactaceae, as seeds of one Mammillaria species (M. lasiacantha) possess only C-lignin, three Escobaria species (E. dasyacantha, E. lloydii and E. zilziana) contain an unusual lignin composed of 5-hydroxyguaiacyl units, the first report of such a polymer that occurs naturally in plants, and seeds of some species contain no lignin at all. We discuss the implications of these findings for the mechanisms that underlie the biosynthesis of these newly discovered lignin types.

  10. Pollination and seed dispersal of Melocactus ernestii Vaupel subsp. ernestii (Cactaceae) by lizards: an example of double mutualism.

    Science.gov (United States)

    Gomes, V G N; Quirino, Z G M; Machado, I C

    2014-03-01

    Recent studies show that the mutualistic role of lizards as pollinators and seed dispersers has been underestimated, with several ecological factors promoting such plant-animal interactions, especially on oceanic islands. Our aim is to provide a quantitative assessment of pollination and seed dispersal mutualisms with lizards in continental xeric habitats. We carried out focal observations of natural populations of Melocactus ernestii (Cactaceae) in the Caatinga, a Brazilian semiarid ecosystem, in order to record the frequency of visits, kind of resource searched and behaviour of visiting animals towards flowers and/or fruits. We made a new record of the lizard Tropidurus semitaeniatus foraging on flowers and fruits of M. ernestii. During the search for nectar, T. semitaeniatus contacted the reproductive structures of the flowers and transported pollen attached to its snout. Nectar production started at 14:00 h, with an average volume of 24.4 μl and an average concentration of solutes of 33%. Approximately 80% of the seeds of M. ernestii found in the faeces of T. semitaeniatus germinated under natural conditions. The roles of T. semitaeniatus as pollinator and seed disperser for M. ernestii show a clear relationship of double mutualism between two endemic species, which may result from the environmental conditions to which both species are subject. Seasonality, low water availability and arthropod supply in the environment, high local lizard densities, continuous nectar production by the flower and fruits with juicy pulp may be influencing the visits and, consequently, pollination and seed dispersal by lizards in this cactus.

  11. Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae).

    Science.gov (United States)

    Alencar, Nara L M; Innecco, Renato; Gomes-Filho, Enéas; Gallão, Maria Izabel; Alvarez-Pizarro, Juan C; Prisco, José T; Oliveira, Alexandre B De

    2012-09-01

    Cereus jamacaru, a Cactaceae found throughout northeast Brazil, is widely used as cattle food and as an ornamental and medicinal plant. However, there has been little information about the physiological and biochemical aspects involved in its germination. The aim of this study was to investigate its reserve mobilization during germination and early seedling growth. For this, C. jamacaru seeds were germinated in a growth chamber and collected at 0, 2, 4, 5, 6, 8 and 12 days after imbibition for morphological and biochemical analyses. Dry seeds had wrinkled seed coats and large, curved embryos. Lipids were the most abundant reserve, comprising approximately 55% and 65% of the dry mass for cotyledons and the hypocotylradicle axis, respectively. Soluble sugars and starch were the minor reserves, corresponding to approximately 2.2% of the cotyledons' dry mass, although their levels showed significant changes during germination. Soluble proteins corresponded to 40% of the cotyledons' dry mass, which was reduced by 81% at the final period of germination compared to dry seeds. C. jamacaru seed can be classified as an oil seed due to its high lipid content. Moreover, lipids were the main reserve mobilized during germination because their levels were strongly reduced after seed germination, while proteins were the second most utilized reserve in this process.

  12. Reproductive biology of a highly endemic species: Cipocereus laniflorus N.P. Taylor & Zappi (Cactaceae Biologia reprodutiva de uma espécie altamente endêmica: Cipocereus laniflorus Taylor & Zappi (Cactaceae

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    Juliana Ordones Rego

    2012-03-01

    Full Text Available Cipocereus laniflorus N.P. Taylor & Zappi is an endemic species from the Serra do Caraça, State of Minas Gerais, Brazil. In order to propose conservation strategies for this species, its reproductive strategies were investigated, including reproductive phenology, floral biology, floral visitors and breeding system. The flowering and fruiting period extends from May to October. Few flowers per plant open each night, producing up to 0.4 ml nectar, but 30% of them are nectarless. Probably pollen is also offered as a resource. Fruiting efficiency of C. laniflorus (47% is close to that found in other Cactaceae species. Pollen of this species was detected in Anoura geoffroy, Soricina glossophaga and Pygoderma bilabiatum bats. Amongst the characteristics related to bat-pollination syndrome found in C. laniflorus, the cream-white colouring of the internal part of the flower, the numerous stamens and the nocturnal anthesis of short duration can be highlighted. Flowers of C. laniflorus are also visited by Nitidulidae beetles, Trigona fulviventris bees and hummingbirds, however bats are the main pollinators of this species. Finally, as a self-sterile species, C. laniflorus needs a pollinator and is more susceptible to the risk of extinction if local disturbances affect its pollination system.Cipocereus laniflorus N.P. Taylor & Zappi é uma espécie endêmica da Serra do Caraça, Minas Gerais, Brasil. A fim de se propor estratégias de conservação para esta espécie, estudos sobre sua biologia reprodutiva foram realizados, incluindo fenologia reprodutiva, biologia floral, visitantes florais e o sistema reprodutivo. O período de floração e frutificação ocorre de maio a outubro. Poucas flores abrem-se por planta a cada noite, podendo produzir até 0.4 ml de néctar, poré 30% destas não apresentam néctar. Cipocereus laniflorus provavelmente oferece também pólen como recurso. A eficiência de frutificação sob condições naturais de poliniza

  13. Insect growth regulatory effects of some extracts and sterols from Myrtillocactus geometrizans (Cactaceae) against Spodoptera frugiperda and Tenebrio molitor.

    Science.gov (United States)

    Céspedes, Carlos L; Salazar, J Rodrigo; Martínez, Mariano; Aranda, Eduardo

    2005-10-01

    A methanol extract from the roots and aerial parts of Myrtillocactus geometrizans (Cactaceae) yielded peniocerol 1, macdougallin 2, and chichipegenin 3. The natural products 1, 2 their mixtures, MeOH and CH(2)Cl(2) extracts showed insecticidal and insect growth regulatory activity against fall armyworm [Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae)], an important insect pest of corn, and [Tenebrio molitor (Coleoptera)], a pest of stored grains in Mexico. The most active compounds were 1, 2, and a mixture (M(2)) of 1 and 2 (6:4). All these extracts, compounds and the mixture had insect growth regulating (IGR) activity between 5.0 and 50.0 ppm and insecticidal effects between 50 and 300 ppm in diets. The extracts were insecticidal to larvae, with lethal doses between 100 and 200 ppm. These compounds appear to have selective effects on the pre-emergence metabolism of Coleoptera, because in all treatments of the larvae of T. molitor, pupation were shortened and this process show precociousness in relation to controls. In contrast to S. frugiperda larvae, onset of pupation was noticeably delayed. Emergence in both cases was drastically diminished. In both pupae and in the few adults that were able to emerge, many deformations were observed. The results of these assays indicated that the compounds were more active than other known natural insect growth inhibitors such as gedunin and methanol extracts of Cedrela salvadorensis and Yucca periculosa. Peniocerol, macdougallin and chichipegenin, as well as mixtures of these substances, may be useful as natural insecticidal agents.

  14. O gênero Rhipsalis Gärtner (Cactaceae, no Estado de São Paulo I: espécies com ramos cilíndricos ou subcilíndricos The genus Rhipsalis Gärtner (Cactaceae in the State of São Paulo-I: species with cylindrical or subcilindrical joints

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    Julio Antonio Lombardi

    1991-12-01

    Full Text Available Rhipsalis Gärtner é um gênero de Cactaceae de hábito epífita ou rupícola, de ampla distribuição nas Américas do Sul e Central, atingindo inclusive a África tropical e ilhas do Oceano Índico. Este trabalho procurou caracterizar morfologicamente as espécies do gênero Rhipsalis que ocorrem no Estado de São Paulo, possibilitando deste modo o reconhecimento das espécies. Entre as espécies do gênero que possuem artículos cilíndricos e subcilíndricos foram reconhecidas 16 espécies ocorrentes no Estado de São Paulo, incluindo uma espécie nova, R. spinescens Lombardi, além de uma espécie de determinação incerta.Rhipsalis Gärtner is a genus of epiphytic or rupicolous Cactaceae, with a wide distribution in South and Central America. It also occurs in tropical Africa and the islands of the Indie Ocean. This study describes the morphology of the species ocurring in São Paulo state (Brazil. A total of 16 species with cylindrical and subcylindrical joints were recognized for São Paulo state, including an new species R. spinescens Lombardi, and another of uncertain affinity.

  15. Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae

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    Nara L.M. Alencar

    2012-09-01

    Full Text Available Cereus jamacaru, a Cactaceae found throughout northeast Brazil, is widely used as cattle food and as an ornamental and medicinal plant. However, there has been little information about the physiological and biochemical aspects involved in its germination. The aim of this study was to investigate its reserve mobilization during germination and early seedling growth. For this, C. jamacaru seeds were germinated in a growth chamber and collected at 0, 2, 4, 5, 6, 8 and 12 days after imbibition for morphological and biochemical analyses. Dry seeds had wrinkled seed coats and large, curved embryos. Lipids were the most abundant reserve, comprising approximately 55% and 65% of the dry mass for cotyledons and the hypocotylradicle axis, respectively. Soluble sugars and starch were the minor reserves, corresponding to approximately 2.2% of the cotyledons' dry mass, although their levels showed significant changes during germination. Soluble proteins corresponded to 40% of the cotyledons' dry mass, which was reduced by 81% at the final period of germination compared to dry seeds. C. jamacaru seed can be classified as an oil seed due to its high lipid content. Moreover, lipids were the main reserve mobilized during germination because their levels were strongly reduced after seed germination, while proteins were the second most utilized reserve in this process.Cereus jamacaru, uma cactácea encontrada comumente no nordeste brasileiro, é amplamente usada como planta forrageira e como ornamental e medicinal. No entanto, existem poucas informações sobre os aspectos fisiológicos e bioquímicos relacionados à sua germinação. O objetivo desse estudo foi avaliar a mobilização de reservas durante a germinação e o crescimento inicial de plântulas de C. jamacaru. Para isso, as sementes foram germinadas em câmaras de germinação e coletadas aos 0, 2, 4, 5, 6, 8 e 12 dias após a germinação para as análises fisiológicas e bioquímicas. As sementes

  16. Distinguishing suitable biotypes of Dactylopius tomentosus (Hemiptera: Dactylopiidae) for biological control of Cylindropuntia fulgida var. fulgida (Caryophyllales: Cactaceae) in South Africa.

    Science.gov (United States)

    Mathenge, C W; Holford, P; Hoffmann, J H; Zimmermann, H G; Spooner-Hart, R; Beattie, G A C

    2009-12-01

    Cylindropuntia fulgida (Engelmann) F.M. Knuth var. fulgida (Engelmann) F.M. Knuth (Cff) (Caryophyllales: Cactaceae) is native to Mexico and Arizona and was introduced into South Africa for ornamental purposes. It subsequently became highly invasive, necessitating control. The cochineal insect, Dactylopius tomentosus (Lamarck) (Hemiptera: Dactylopiidae), was selected as a potential biological control agent based on its restricted host range among Cylindropuntia species and previous success in controlling C. imbricata (DC.) F. Knuth (Ci). Eight D. tomentosus provenances (Cholla, Cholla E, Fulgida, Mamillata, Imbricata, Tunicata U, Tunicata V and Rosea) from Cylindropuntia species in their native ranges were reared on Cff, whilst Cholla and Imbricata were also reared on Ci. Large differences were found in the development and survival of crawlers, and in the reproductive capacity of females. Three subjective categories of provenance interaction with host plants were identified based on a fitness index (FI) calculated from data relating to crawler survival, female development time and fecundity: (i) thriving (FI > or = 1) - insects had shorter developmental times, high crawler survival and highly fecund females (Cholla); (ii) surviving (FI0) - insects had extended development times, low crawler survival and low fecundity (Imbricata, Fulgida and Mamillata); and (iii) dying (FI = 0) - insects died before or at the second instar (Rosea, Tunicata U and Tunicata V). Cholla, therefore, is highly suitable for biological control of Cff in South Africa. In addition, Cholla thrived on Cff but only survived on Ci whilst, in contrast, Imbricata thrived on Ci but only survived on Cff. This differential ability of provenances to thrive or survive on different host plants demonstrated that host adapted biotypes of D. tomentosus exist; therefore, biotypes should be taken into account when considering this species as a biological control agent of cactus weeds.

  17. Efecto de diferentes reguladores de crecimiento vegetal sobre la germinación de semillas y desarrollo de plántulas de dos especies de Ferocactus (Cactaceae

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    Karina Amador-Alférez

    2015-08-01

    Full Text Available Se evaluó el efecto de la adición de tres regu-ladores de crecimiento vegetal (RCV sobre la respuesta germinativa y crecimiento de plántulas en las especiesFerocactus histrixyF. latispinus(Cactaceae. Grupos de semillas se germinaron con la adición de diferentes concentraciones (125, 250, 500 ppm de áci-do indolacético (AIA, ácido naftalenacético (ANA y ácido giberélico (AG3, mientras que otras semillas (control se sembraron con agua destilada solamente. Cada tercer día y durante 30 días se llevó a cabo el registro de porcentaje de germinación (%G, el índice de velocidad de germinación (IVG y de coeficientes de regresión; los caracteres morfológicos durante el crecimiento inicial de las plántulas provenientes de dichos experimentos también fueron registrados. Los resultados demostraron diferencias estadísticas significativas (Tukey, P Ferocactus, se registraron en el tratamiento control. El AIA a una concentración de 125 ppm promovió 51% de germinación enF. latispinusal noveno día del experimento, un 62% de germinación se registró paraF. histrixen el sexto día con 250 ppm de ANA; mientras que el porcentaje de germinación más bajo (

  18. Triatominae et Cactaceae : un risque pour la transmission de la Trypanosomose américaine dans le péridomicile (nord-est du Brésil

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    Emperaire L.

    2006-06-01

    Full Text Available Les observations de terrain réalisées dans le nord-est du Brésil ont montré la fréquente association, dans l’espace péridomiciliaire, entre un cactus, le Cereus jamacaru, la présence de nids dans ses branches et celle de Rhodnius neglectus et de Triatoma pseudomaculata, espèces vectrices du parasite Trypanosoma cruzi, agent de la maladie de Chagas. L’analyse des variables architecturales de cette Cactaceae montre que la présence de nids, et donc d’insectes, est inféodée aux pratiques traditionnelles de gestion de ce cactus. Cette étude souligne l’intérêt d’une approche intégrée de l’écologie des Triatominae pour l’identification des variables indicatrices de risque.

  19. Anatomy of Brazilian Cereeae (subfamily Cactoideae, Cactaceae: Arrojadoa Britton & Rose, Stephanocereus A. Berger and Brasilicereus Backeberg Anatomia de espécies brasileiras pertencentes à tribo Cereeae (subfamília Cactoideae, Cactaceae: Arrojadoa Britton & Rose, Stephanocereus A. Berger and Brasilicereus Backeberg

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    Patricia Soffiatti

    2007-12-01

    Full Text Available (Anatomy of Brazilian Cereeae (subfamily Cactoideae, Cactaceae: Arrojadoa Britton & Rose, Stephanocereus A. Berger wâBrasilicereus Backeberg. Arrojadoa, Stephanocereus and Brasilicereus are endemic Brazilian Cereeae, occurring along the Espinhaço Range, in the campos rupestres, cerrados and caatingas, from northern Minas Gerais to southern Bahia. The genera are columnar, erect to semi-erect cacti, except for one species, A bahiensis, which is globose. This study describes the anatomy of dermal, fundamental and vascular systems, aiming to find diagnostic characters for the genera and species. Basal portions of stems were sectioned transversely and longitudinally, and stained with Astrablue and Safranin. The species share a uniseriate epidermis, with thick cuticle; well developed collenchymatic hypodermis, containing prismatic crystals; cortex with numerous mucilage cells, druses and vascular bundles; outside cortex as a palisade parenchyma; periderm composed of lignified cork cells alternating with suberized cells; pheloderm consisting of a few layers of thin-walled cells; phloem composed of solitary or multiple of two to three sieve tube elements, companion cells, axial and radial parenchyma; secondary xylem with solitary to multiple vessels, with simple perforation plates and alternate bordered to semi-bordered pits; axial parenchyma scanty vasicentric to incomplete; libriform septate fibres; large rays. Unlignified parenchyma is seen in the secondary xylem, varying from a few cells to bands among axial and radial elements. The following are considered diagnostic characters: the shape of lignified phellem cells, cubic to radially elongate, which individualizes S. leucostele; an underdeveloped hypodermis and the occurrence of sclereids in the cortex are exclusive to Brasilicereus markgrqfii.(Anatomia de espécies brasileiras pertencentes à tribo Cereeae (subfamília Cactoideae, Cactaceae: Arrojadoa Britton & Rose, Stephanocereus A. Berger and

  20. Padrões de isozimas de malato desidrogenase em população clonal nos cladófilos de Opuntia ficus-indica Mill (Cactaceae - DOI: 10.4025/actascibiolsci.v25i1.2093 Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093

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    Alexandro Cezar Faleiro

    2003-04-01

    Full Text Available Isozimas de malato desidrogenase (MDH foram usadas como marcadores moleculares para discriminar e agrupar cladófilos de plantas de uma população clonal de cactus da espécie Opuntia ficus-indica (Cactaceae, conhecida como palma. O padrão eletroforético obtido revelou 8 isozimas MDH e 5 fenótipos eletroforéticos diferentes. A similaridade entre os cladófilos foi estimada usando o coeficiente de similaridade de Jaccard. Essa população clonal estudada foi fundada por somente um propágulo e, após 50 anos, parece ser formada por propágulos assexuais e sexuais. Uma vez que a expressão diferencial de isozimas MDH pode ter um papel significante no metabolismo das células da planta, sugerimos que os cladófilos de palma que foram agrupados com os mais altos valores de similaridade são os mais adequados para serem utilizados em procedimentos de extração industrial de compostos de interesse comercial, porque um mesmo protocolo de extração pode ser mais rapidamente e facilmente padronizado quando se utiliza material geneticamente uniforme. O padrão eletroforético das isozimas MDH pode ser usado como uma ferramenta efetiva para uma análise prévia da similaridade genética entre os cladófilos das plantas de O. ficus-indicaMalate dehydrogenase (MDH isozymes were used as biochemical markers to discriminate and cluster cladophylls of plants of one clonal population of the prickly pear, Opuntia ficus-indica (Cactaceae. The isozyme electrophoretic patterns obtained with MDH provided 8 isozymes and 5 different electrophoretic phenotypes. Similarity in cladophylls was estimated using Jaccard’s coefficient. This clonal population studied was founded by only one propagule, and after 50 years, it is likely to have been formed by asexual and sexual propagules. Since that differential expression of MDH isozymes could play a significant role in overall plant cell metabolism, we suggest that the cladophylls of prickly pear that were clustered

  1. Bone Mineral Density, Mechanical, Microstructural Properties and Mineral Content of the Femur in Growing Rats Fed with Cactus Opuntia ficus indica (L.) Mill. (Cactaceae) Cladodes as Calcium Source in Diet.

    Science.gov (United States)

    Hernández-Becerra, Ezequiel; Gutiérrez-Cortez, Elsa; Del Real, Alicia; Rojas-Molina, Alejandra; Rodríguez-García, Mario; Rubio, Efraín; Quintero-García, Michelle; Rojas-Molina, Isela

    2017-02-04

    Mechanical, microstructural properties, mineral content and bone mineral density (BMD) of the femur were evaluated in growing rats fed with Opuntia ficus indica (L.) Mill. (Cactaceae) cladodes at different maturity stages as calcium source. Male weanling rats were fed with cladodes at early maturity stage (25 and 60 days of age, belonging to groups N-60 and N-200, respectively) and cladodes at late maturity stage (100 and 135 days of age, belonging to groups N-400 and N-600, respectively) for 6 weeks. Additionally, a control group fed with calcium carbonate as calcium source was included for comparative purposes. All diets were fitted to the same calcium content (5 g/kg diet). The failure load of femurs was significantly lower (p ≤ 0.05) in groups N-60 and N-200 in comparison to N-400, N-600 and control groups. The cortical width (Ct.Wi) and trabecular thickness (Tb.Th) of the femurs in control and N-600 groups were significantly higher (p ≤ 0.05) than Ct.Wi and Tb.Th of femurs in groups N-60 and N-200. Trabecular separation of the femurs in N-60 and N-200 groups showed the highest values compared with all experimental groups. The highest calcium content in the femurs were observed in control, N-600 and N-400 groups; whereas the lowest phosphorus content in the bones were detected in N-200, N-600 and N-400 groups. Finally, the BMD in all experimental groups increased with age; nevertheless, the highest values were observed in N-600 and control groups during pubertal and adolescence stages. The results derived from this research demonstrate, for the first time, that the calcium found in Opuntia ficus indica cladodes is actually bioavailable and capable of improving mineral density and mechanical and microstructural properties of the bones. These findings suggest that the consumption of cladodes at late maturity stage within the diet might have a beneficial impact on bone health.

  2. Bone Mineral Density, Mechanical, Microstructural Properties and Mineral Content of the Femur in Growing Rats Fed with Cactus Opuntia ficus indica (L.) Mill. (Cactaceae) Cladodes as Calcium Source in Diet

    Science.gov (United States)

    Hernández-Becerra, Ezequiel; Gutiérrez-Cortez, Elsa; Del Real, Alicia; Rojas-Molina, Alejandra; Rodríguez-García, Mario; Rubio, Efraín; Quintero-García, Michelle; Rojas-Molina, Isela

    2017-01-01

    Mechanical, microstructural properties, mineral content and bone mineral density (BMD) of the femur were evaluated in growing rats fed with Opuntia ficus indica (L.) Mill. (Cactaceae) cladodes at different maturity stages as calcium source. Male weanling rats were fed with cladodes at early maturity stage (25 and 60 days of age, belonging to groups N-60 and N-200, respectively) and cladodes at late maturity stage (100 and 135 days of age, belonging to groups N-400 and N-600, respectively) for 6 weeks. Additionally, a control group fed with calcium carbonate as calcium source was included for comparative purposes. All diets were fitted to the same calcium content (5 g/kg diet). The failure load of femurs was significantly lower (p ≤ 0.05) in groups N-60 and N-200 in comparison to N-400, N-600 and control groups. The cortical width (Ct.Wi) and trabecular thickness (Tb.Th) of the femurs in control and N-600 groups were significantly higher (p ≤ 0.05) than Ct.Wi and Tb.Th of femurs in groups N-60 and N-200. Trabecular separation of the femurs in N-60 and N-200 groups showed the highest values compared with all experimental groups. The highest calcium content in the femurs were observed in control, N-600 and N-400 groups; whereas the lowest phosphorus content in the bones were detected in N-200, N-600 and N-400 groups. Finally, the BMD in all experimental groups increased with age; nevertheless, the highest values were observed in N-600 and control groups during pubertal and adolescence stages. The results derived from this research demonstrate, for the first time, that the calcium found in Opuntia ficus indica cladodes is actually bioavailable and capable of improving mineral density and mechanical and microstructural properties of the bones. These findings suggest that the consumption of cladodes at late maturity stage within the diet might have a beneficial impact on bone health. PMID:28165410

  3. Bone Mineral Density, Mechanical, Microstructural Properties and Mineral Content of the Femur in Growing Rats Fed with Cactus Opuntia ficus indica (L. Mill. (Cactaceae Cladodes as Calcium Source in Diet

    Directory of Open Access Journals (Sweden)

    Ezequiel Hernández-Becerra

    2017-02-01

    Full Text Available Mechanical, microstructural properties, mineral content and bone mineral density (BMD of the femur were evaluated in growing rats fed with Opuntia ficus indica (L. Mill. (Cactaceae cladodes at different maturity stages as calcium source. Male weanling rats were fed with cladodes at early maturity stage (25 and 60 days of age, belonging to groups N-60 and N-200, respectively and cladodes at late maturity stage (100 and 135 days of age, belonging to groups N-400 and N-600, respectively for 6 weeks. Additionally, a control group fed with calcium carbonate as calcium source was included for comparative purposes. All diets were fitted to the same calcium content (5 g/kg diet. The failure load of femurs was significantly lower (p ≤ 0.05 in groups N-60 and N-200 in comparison to N-400, N-600 and control groups. The cortical width (Ct.Wi and trabecular thickness (Tb.Th of the femurs in control and N-600 groups were significantly higher (p ≤ 0.05 than Ct.Wi and Tb.Th of femurs in groups N-60 and N-200. Trabecular separation of the femurs in N-60 and N-200 groups showed the highest values compared with all experimental groups. The highest calcium content in the femurs were observed in control, N-600 and N-400 groups; whereas the lowest phosphorus content in the bones were detected in N-200, N-600 and N-400 groups. Finally, the BMD in all experimental groups increased with age; nevertheless, the highest values were observed in N-600 and control groups during pubertal and adolescence stages. The results derived from this research demonstrate, for the first time, that the calcium found in Opuntia ficus indica cladodes is actually bioavailable and capable of improving mineral density and mechanical and microstructural properties of the bones. These findings suggest that the consumption of cladodes at late maturity stage within the diet might have a beneficial impact on bone health.

  4. Triterpenoid saponins from Echinopsis macrogona (Cactaceae).

    Science.gov (United States)

    Okazaki, Sachie; Kinoshita, Kaoru; Ito, Satoru; Koyama, Kiyotaka; Yuasa, Hiroshi; Takahashi, Kunio

    2011-01-01

    Triterpene saponins, pachanosides C1, E1, F1 and G1 (1-4), and bridgesides A1, C1, C2, D1, D2, E1 and E2 (5-11) were isolated from Echinopsis macrogona. Compounds 1-4 were saponins with pachanane type triterpene saponins, while the others (5-11) were oleanane type triterpene saponins. While the aglycones of 2-4 and 8-11 were hitherto unknown, the structure of pachanol C was revised in this paper. Their structures were elucidated on the basis of chemical and physicochemical evidence.

  5. Atividade antibacteriana de Cereus jamacaru DC, Cactaceae

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    Aline Davet

    2009-06-01

    Full Text Available A procura por novos agentes antimicrobianos a partir de plantas vem sendo intensificada e não existem estudos direcionados à avaliação da atividade antimicrobiana de C. jamacaru frente a microrganismos prejudiciais ao homem. Em vista disso, avaliou-se o efeito antimicrobiano do extrato bruto etanólico do lenho (EBLE e do córtex (EBCO desta espécie frente a colônias puras de oito microrganismos patogênicos. Neste estudo foram utilizados os métodos difusão em ágar (meio sólido e CIM (concentração inibitória mínima. Os resultados obtidos neste ensaio preliminar mostraram que EBCO apresentou atividade antimicrobiana mais pronunciada que EBCE e que os microrganismos cujo crescimento foi mais influenciado por EBCO foram Streptococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli.

  6. A new combination in Peniocereus (Cactaceae Una nueva combinación en Peniocereus (Cactaceae

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    Carlos Gómez-Hinostrosa

    2005-12-01

    Full Text Available Acanthocereus chiapensis Bravo was described from plants collected in Chiapas, Mexico in March 1967. Field observations as well as detailed morphological and anatomical studies revealed that this entity rather belongs to genus Peniocereus. This taxonomic opinion was supported by the presence in this plant of tuberous roots; relatively thin stems; relatively small flowers and fruits, with numerous, weak spines; absence of primary phloem fiber caps on cortical bundles; and presence of minute, inter-cellular interstices in the seeds. Consequently, the following new combination is made herein: P. chiapensis (Bravo C. Gómez-Hinostrosa and H. M. Hernández.Acanthocereus chiapensis Bravo fue descrita con base en plantas recolectadas en Chiapas, México en Marzo de 1967. Observaciones en el campo así como estudios morfológicos y anatómicos detallados, revelaron que esta entidad pertenece más bien al género Peniocereus. Esta opinión taxonómica está apoyada por la presencia en esta planta de raíces tuberosas; tallos relativamente angostos; flores y frutos relativamente pequeños, con numerosas espinas frágiles; ausencia de fibras sobre el floema primario de los haces corticales; y presencia de intersticios inter-celulares en las semillas. En consecuencia se hace la siguiente combinación nueva: P. chiapensis (Bravo C. Gómez-Hinostrosa and H. M. Hernández.

  7. Correlation Between the Negative Air Ions Released by 3 Species in Cactaceae and the Releasing Passage%3种仙人掌科植物负离子释放量与释放通道的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张万超; 郑俊鸣; 丁旭玲; 彭东辉; 吴仁烨; 邓传远; 郑金贵

    2016-01-01

    在室内密闭玻璃箱内测定了3种仙人掌科(Cactaceae)植物,即银冠(Cotyledon orbiculata var.oblonga)、玉翁(Mammillaria hahniana)和金晃(Notocactus leninghausii),在高压脉冲电刺激下不同剪刺阶段负离子释放浓度、刺数量和气孔数量特征,光镜观察了不同剪刺阶段气孔的形态特征,探讨了高压脉冲电场刺激下仙人掌科植物负离子释放量与释放通道的相关性.结果表明:在高压脉冲电刺激下,银冠负离子浓度随气孔长度的增大而升高(p<0.01),玉翁负离子浓度随其刺数量的减少而降低(p<0.0l),随气孔长度的增大而升高(p<0.01),金晃负离子浓度随其刺数量的减少而降低(p<0.01),随气孔宽长比的增大而升高(p<0.01).从结果推断,在高压脉冲电刺激下,仙人掌科植物释放负离子的过程受其生理活动的影响;仙人掌科植物的刺尖和气孔都是其释放空气负离子的通道,当植物生理活动强度大到改变了其气孔数量特征参数时,气孔就可能成为主要的释放通道.

  8. [Sexual compatibility between two types of Hylocerus (Cactaceae)].

    Science.gov (United States)

    Castillo, Roberta; Livera, Manuel; Brechú, Alicia E; Márquez-Guzmán, Judith

    2003-01-01

    There are two types of pitahaya that are cultivated in Yucatan Peninsula of Mexico. They differ mainly in the skin color of the fruit, one of them has a red skin (Uqroo1), while the other has a light yellow skin (Uqroo2) both belong to Hylocereus undatus (Haworth) Britt. & Rose. The yellow skin pitahaya is the sweetest. The studies were conducted using the methods of self pollination and cross pollination, with direct crosses and reciprocal pollination in order to evaluate the effect related to the quantity of fruits that accomplish their growing process. Some characteristic parameters of fruits, thus obtained, were used to detect the possible xenic effect. The control samples were collected from naturally pollinated flowers. The experimental design used was completely random and the experiment was repeated fifteen times. The results revealed that the Uqroo1 turned out to be self-compatible, and their fruits reached one hundred percent of development. On the other hand, Uqroo2 was self-incompatible, that is, it did not accept self pollen. The control samples of both types reached the same weight, and the fruits of the Uqroo2 produced by cross pollination had the same or more weight than the control samples. Several parameters were positively correlated. The coefficient between the number of seeds and the fruit weight was r = 0.84. The highest correlation that was found (r = 0.97) in the fruit weight and the pulp weight within both types. The fruits obtained by cross-pollination maintained the characteristic of the female progenitor. It is suggest intercalate the yellow pitahaya plants with another types or species of pitahaya, other than the Uqroo1.

  9. Taxonomic revalidation and potential distribution of Armatocereus brevispinus Madsen (Cactaceae

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    Christian R. Loaiza S.

    2016-05-01

    Full Text Available We report the first confirmed record for northern Peru of Armatocereus brevispinus Madsen, a species of cactus regarded as endemic to the province of Loja, in the southern region of Ecuador. The record corresponds to a locality placed near the km 18 of the route to Ayabaca (04º42’21.0”S and 79º48’51.0”W, in the district of Ayabaca, within the province of Ayabaca, in the Department of Piura. This records represents an extension of the distributional range known for this species in Ecuador. We analyze its taxonomy and the distribution and conservation status and comments on its biogeography in the Andean region.

  10. How specialised is bird pollination in the Cactaceae?

    Science.gov (United States)

    Gorostiague, P; Ortega-Baes, P

    2016-01-01

    Many cactus species produce 'bird' flowers; however, the reproductive biology of the majority of these species has not been studied. Here, we report on a study of the pollination of two species from the Cleistocactus genus, cited as an ornithophilous genus, in the context of the different ways in which they are specialised to bird pollination. In addition, we re-evaluate the level of specialisation of previous studies of cacti with bird pollination and evaluate how common phenotypic specialisation to birds is in this family. Both Cleistocactus species exhibited ornithophilous floral traits. Cleistocactus baumannii was pollinated by hummingbirds, whereas Cleistocactus smaragdiflorus was pollinated by hummingbirds and bees. Pollination by birds has been recorded in 27 cactus species, many of which exhibit ornithophilous traits; however, they show generalised pollination systems with bees, bats or moths in addition to birds being their floral visitors. Of all cactus species, 27% have reddish flowers. This trait is associated with diurnal anthesis and a tubular shape. Phenotypic specialisation to bird pollination is recognised in many cactus species; however, it is not predictive of functional and ecological specialisation in this family.

  11. Cactaceae endémicas del Perú

    Directory of Open Access Journals (Sweden)

    Mónica Arakaki

    2013-10-01

    Full Text Available 250 especies (Brako & Zarucchi, 1993; Ulloa Ulloa et al., 2004, mayormente cactus arbustivo-columnares. En este trabajo reconocemos 199 endemismos en 32 géneros. Seis géneros, Calymnanthium, Lasiocereus, Matucana, Mila, Oroya y Pygmaeocereus son endémicos al Perú. Esta familia requiere de esfuerzos metódicos para incrementar su representación en los herbarios nacionales, asociados con una evaluación de las poblaciones y de sus hábitats, así como una evaluación de la taxonomía y sistemática de estos taxones. La mayoría de los taxones endémicos ocupan las regiones Matorral Desértico y Mesoandina, desde el nivel del mar hasta los 4000 m de altitud. Se aplicaron las categorías y criterios de la UICN a 58 taxones. Quince taxones endémicos están representados dentro del Sistema Nacional de Áreas Naturales Protegidas por el Estado.

  12. Seed morphology and variation in the genus Pachycereus (Cactaceae).

    Science.gov (United States)

    Arias, Salvador; Terrazas, Teresa

    2004-08-01

    Seeds of 13 Pachycereus species and two Stenocereus species that have been suggested as closely related were examined with the scanning electron microscope. Quantitative features were evaluated using multivariate analysis in order to identify characters that distinguish them. Several species groups were recognized on the basis of 16 qualitative characters. All species studied are keeled. Stenocereus aragonii and S. eichlamii share with most Pachycereus species large size, glossy appearance, and a flat relief on periclinal cells in the lateral region. Pachycereus gatesii and P. schottii are unique in having the smallest seeds and a deeply impressed hilum-micropylar region. P. hollianus does not exhibit micro-relief on periclinal walls in the lateral region, and P. fulviceps has no expanded testa border. Multivariate analysis showed that four characters, length, breadth, hilum-micropylar region length, and angle, made the greatest contribution to distinguishing among species groups. More than 80% of P. fulviceps, P. hollianus, P. tepamo, P. weberi, and S. eichlamii seeds could be classified correctly using four seed features and the percentage was even higher using just two or three features for P. gatesii, P. grandis, P. militaris, P. pringlei, and P. schottii. Testa appearance, testa cell-pattern, and position relative to the rim of the hilum-micropylar region were found to be potentially informative and should be combined with other sources of data in future phylogenetic analyses.

  13. Rhipsalis grandiflora Haw. (Cactaceae propagation by setem cuttings

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    Stancato Giulio Cesare

    2003-01-01

    Full Text Available The genus Rhipsalis is being cultivated as a potted plant since the view of the pendant branches with coloured flowers and fruit is very appreciated. Considering that there is no information about the production of rooted propagules of these species, the goal of this work was to evaluate the development of stem cuttings, stimulated by the application of plant growth regulators. The indolebutyric acid (IBA was tested at 0; 4.07; 5.81, and 11.63 mmol L-1 and the naphthaleneacetic acid (NAA at 0; 4.53; 6.47, and 12.94 mmol L-1 for their activity in promoting roots on apical stem segments of Rhipsalis grandiflora Raw. The base of stem cuttings was dipped into auxin solutions mixed with talc and each one was partially embedded in fir bark (Pinus sp and fern fiber (1:1 in polystyrene propagation flats, placed throughout the rooting period in a greenhouse (50% shading, at 60-90% of relative humidity and temperatures from 20 to 25ºC. The experimental design was a completely randomised with eight treatments (twelve replicates, with samplings at 0; 20; 50; 80, and 150 days. The results indicate no differences between IBA and NAA regarding the dry mass of roots, but an increase in for concentrations of 0 and 4.07 mmol L-1 of IBA and 0 and 4.53 mmol L-1 of NAA, in relation to treatments with auxins. Stem cuttings treated with 4.07 mmol L-1 of IBA and 4.53 mmol L-1 of NAA, had greater dry mass of shoot bud differentiation in relation to those treated with 0 mmol L-1.

  14. Population viability of Pediocactus brady (Cactaceae) in a changing climate

    Science.gov (United States)

    Shryock, Daniel F.; Esque, Todd C.; Huges, Lee

    2014-01-01

    • Premise of the study: A key question concerns the vulnerability of desert species adapted to harsh, variable climates to future climate change. Evaluating this requires coupling long-term demographic models with information on past and projected future climates. We investigated climatic drivers of population growth using a 22-yr demographic model for Pediocactus bradyi, an endangered cactus in northern Arizona.

  15. Pereskia aculeata Muller (Cactaceae Leaves: Chemical Composition and Biological Activities

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    Lucèia Fàtima Souza

    2016-09-01

    Full Text Available The aims of this work were to study the chemical composition of the essential oil from the leaves of Pereskia aculeata and to evaluate some biological activities of three leaf extracts. The phenolic content, antioxidant activity, and in vitro antimicrobial and antifungal activities were determined. The methanol extract showed antioxidant activity (EC50 7.09 mg/mL and high polyphenols content (15.04 ± 0.31 mg gallic acid equivalents (GAE/g. The petroleum ether extract exhibited potent antibacterial activity against Escherichia coli, whereas the chloroform extract showed inhibitory activity against Bacillus cereus and Staphylococcus aureus. The petroleum ether and methanol extracts were more effective in inhibiting the growth of Aspergillus versicolor. The possible cytotoxicity of extracts on neuroblastoma SH-SY5Y cancer cell line and the influence on adenylate cyclase (ADCY expression was also studied. P. aculeata chloroform extract showed antiproliferative activity with an IC50 value of 262.83 µg/mL. Treatments of SH-SY5Y neuroblastoma cells with 100 µg/mL of methanol extract significantly reduced ADCY1 expression.

  16. Alkaloid production by callous tissue cultures of Cereus peruvianus (Cactaceae).

    Science.gov (United States)

    de Oliveira, Arildo José Braz; Machado, Maria Fátima Pires da Silva

    2003-02-01

    The morphologically undifferentiated cells of nonregenerant callous tissue of Cereus peruvianus cultured in the original medium and in medium supplemented with tyrosine were used as an alkaloid source. Comparison of alkaloid production by C. peruvianus plants and by callous tissues indicated that alkaloid levels were almost twice as high in callous tissues as in shoots of C. peruvianus plants. The ratio of alkaloid concentration between mature plant and morphologically undifferentiated cells of callous tissue was 1:1.7. A relationship between culture medium containing tyrosine and alkaloid production was also observed in the callous tissues of C. peruvianus. Since increased alkaloid production may be induced by additional factors such as tyrosine, increasing levels of tyrosine or other conditions of the culture medium may be considered factors for inducing higher alkaloid production by C. peruvianus callous tissues.

  17. Pereskia aculeata Muller (Cactaceae) Leaves: Chemical Composition and Biological Activities.

    Science.gov (United States)

    Souza, Lucèia Fàtima; Caputo, Lucia; Inchausti De Barros, Ingrid Bergman; Fratianni, Florinda; Nazzaro, Filomena; De Feo, Vincenzo

    2016-09-03

    The aims of this work were to study the chemical composition of the essential oil from the leaves of Pereskia aculeata and to evaluate some biological activities of three leaf extracts. The phenolic content, antioxidant activity, and in vitro antimicrobial and antifungal activities were determined. The methanol extract showed antioxidant activity (EC50 7.09 mg/mL) and high polyphenols content (15.04 ± 0.31 mg gallic acid equivalents (GAE)/g). The petroleum ether extract exhibited potent antibacterial activity against Escherichia coli, whereas the chloroform extract showed inhibitory activity against Bacillus cereus and Staphylococcus aureus. The petroleum ether and methanol extracts were more effective in inhibiting the growth of Aspergillus versicolor. The possible cytotoxicity of extracts on neuroblastoma SH-SY5Y cancer cell line and the influence on adenylate cyclase (ADCY) expression was also studied. P. aculeata chloroform extract showed antiproliferative activity with an IC50 value of 262.83 µg/mL. Treatments of SH-SY5Y neuroblastoma cells with 100 µg/mL of methanol extract significantly reduced ADCY1 expression.

  18. Micro-morphology and anatomy of Turbinicarpus (Cactaceae spines Micromorfología y anatomía de las espinas de Turbinicarpus (Cactaceae

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    Alessandro Mosco

    2009-04-01

    Full Text Available Spines are a striking feature of cacti and display wide variation in size, number, shape, and texture. This study showed that Turbinicarpus species exhibit not only a high variability in the gross morphology of the spines, but also in their micro-morphology. Their surface can be smooth or ornamented with projections that can be low, conical, pinnate, or long trichomes. The epidermis can be continuous, broken up into single cell elements or transversely fissured, the fissures extending deeply into the underlying sclerenchyma. The mechanical properties of the spines are related to their anatomy, here documented for the first time. The woody rigid spines being made up of fibers with thick walls (> 3 µm, while papery or corky spines have a sclerenchyma made up of fibers with thin walls (Las espinas son una de las características más distintivas de las cactáceas y se distinguen por su variación en tamaño, número, forma y textura. Este estudio muestra que las especies de Turbinicarpus no sólo tienen variación en la morfología de sus espinas, sino también en su micro-morfología. Su superficie puede ser lisa u ornamentada con proyecciones bajas, cónicas, pinadas o bien con tricomas largos. En las espinas, la epidermis se mantiene continua, separada en sus células o transversalmente fisurada. Las fisuras de la epidermis pueden prolongarse hasta el esclerénquima más interno. Las propiedades mecánicas de las espinas están relacionadas con su anatomía, aquí documentada por primera vez. Las espinas rígidas están constituidas de fibras con paredes gruesas (> 3 µm, mientras que las espinas suaves o corchosas, también denominadas cerdas tienen esclerénquima de fibras con paredes delgadas (< 2 µm. Además, algunas espinas en su madurez tienen 2 tipos de fibras, las pobremente lignificadas en la parte externa y en la interna las de paredes gruesas y lignificadas. La taxonomía de Turbinicarpus se basa principalmente en las espinas y los datos aquí generados contribuyen a un mejor entendimiento de sus relaciones interespecíficas. El conocimiento de las características epidermales como los tricomas largos, la carencia de ornamentación o las modificaciones anatómicas de las espinas corchosas pueden interpretarse como caracteres apomórficos en el género.

  19. 77 FR 61835 - Endangered and Threatened Wildlife and Plants; Endangered Species Status for Cape Sable...

    Science.gov (United States)

    2012-10-11

    ... and demographic trends discussed above. Consolea corallicola Consolea corallicola (Family: Cactaceae... the Species below). Harrisia aboriginum Description Harrisia aboriginum (Family: Cactaceae) is...

  20. [Floral biology and pollinators of Trichocereus pasacana (Cactaceae) in Parque Nacional Los Cardones, Argentina].

    Science.gov (United States)

    de Viana, M L; Ortega Baes, P; Saravia, M; Badano, E I; Schlumpberger, B

    2001-03-01

    Many columnar cacti are bat pollinated. It has been suggested that this kind of pollination would be more important in tropical than in temperate regions where flowers are open only one night. Thrichocereus pasacana produces big and resistant white flowers. We analyzed flower characteristics, floral cycle, stigmatic receptivity, nectar production, pollen presence and floral visitors in a T. pasacana population at National Park Los Cardones (Salta, Argentina) in November 1997. Flower features were constant between individuals of the population. Flowers start opening at evening and anthesis time is from 18 to 40 hs. The estigma was receptive throughout the floral cycle. Anther dehiscence occurs with flower opening. Nectar production was highest between 18 to 24 hs. Although T. pasacana are open during the night, floral visitors are diurnal. The most frequent was Xylocopa sp. In the study area, nectarivorous bats were not detected. The morphological features of T. pasacana flowers were similar but bigger compared to other columnar cacti. Anthesis time was also longer while nectar production was lower. T. pasacana pollination at National Park Los Cardones is done by bees.

  1. [Estimation of mescaline and pellotine in Lophophora coulter plants (Cactaceae) by means of the oscillographic polarography].

    Science.gov (United States)

    Gabermann, V

    1978-02-01

    Oscillographic polarography has been applied for the mescaline and pellotine estimation. These alkaloids produce in 0.5 N NaOH electrolyte a sharp peak within the cathode region of the oscillogram, each of them showing different potential. It makes possible to estimate them at a concentration of 5.10(-6) g/ml. All the forms of Lophophora williamsii were found to contain mescaline and lower content of pellothine, L. jourdaniana--to have equal content of both alkaloide, L. diffusa and L. fricii--to contain pellotine and only traces of mescaline. Plants grown in the greenhouse accumulated the same amount of alkaloids as native plants. Grafting on roodstock which does not produce essential amount of the alkaloids, does not affect the ability of Lophophora to synthesize mescaline and pellotine.

  2. MORFOLOGÍA POLÍNICA DE ESPECIES ARGENTINAS DE REBUTIA S. STR. (CACTACEAE, CACTOIDEAE)

    OpenAIRE

    Silvina Garralla; Nora B. Muruaga; Graciela Cuadrado

    2008-01-01

    Este trabajo analiza las características palinológicas de Rebutia deminuta, R. fabrisii var. fabrisii, R. fabrisii var. aureiflora, R. fiebrigii, R. marsoneri, R. minuscula subsp. minuscula, R. minuscula subsp. wessneriana y R. padcayensis con la finalidad de aportar datos que permitan la delimitación de estas especies. El material polínico procede de ejemplares coleccionados en distintas localidades montanas del noroeste argentino. Las observaciones, conteos y mediciones de los granos de ...

  3. [Bacillus isolates from rhizosphere of cacti improve germination and bloom in Mammillaria spp. (Cactaceae)].

    Science.gov (United States)

    Chávez-Ambriz, Lluvia A; Hernández-Morales, Alejandro; Cabrera-Luna, José A; Luna-Martínez, Laura; Pacheco-Aguilar, Juan R

    Cacti are the most representative vegetation of arid zones in Mexico where rainfall is scarce, evapotranspiration is high and soil fertility is low. Plants have developed physiological strategies such as the association with microorganisms in the rhizosphere zone to increase nutrient uptake. In the present work, four bacterial isolates from the rhizosphere of Mammillaria magnimamma and Coryphantha radians were obtained and named as QAP3, QAP19, QAP22 and QAP24, and were genetically identified as belonging to the genus Bacillus, exhibiting in vitro biochemical properties such as phosphate solubilization, indoleacetic acid production and ACC deaminase activity related to plant growth promotion, which was tested by inoculating M. magnimamma seeds. It was found that all isolates increased germination from 17 to 34.3% with respect to the uninoculated control seeds, being QAP24 the one having the greatest effect, accomplishing the germination of viable seeds (84.7%) three days before the control seeds. Subsequently, the inoculation of Mammillari zeilmanniana plants with this isolate showed a positive effect on bloom, registering during two months from a one year period, an increase of up to 31.0% in the number of flowering plants compared to control plants. The characterized Bacillus spp. isolates have potential to be used in conservation programs of plant species from arid zones.

  4. Stability of some Cactaceae proteins based on fluorescence, circular dichroism, and differential scanning calorimetry measurements.

    Science.gov (United States)

    Gorinstein, S; Zemser, M; Vargas-Albores, F; Ochoa, J L; Paredes-Lopez, O; Scheler, C; Aksu, S; Salnikow, J

    1999-02-01

    Characterization of three cactus proteins (native and denatured) from Machaerocereus gummosus (Pitahaya agria), Lophocereu schottii (Garambullo), and Cholla opuntia (Cholla), was based on electrophoretic, fluorescence, CD (circular dichroism), DSC (differential scanning calorimetry), and FT-IR (Fourier transform infrared) measurements. The obtained results of intrinsic fluorescence, DSC, and CD were dissimilar for the three species of cactus, providing evidence of differences in secondary and tertiary structures. Cactus proteins may be situated in the following order corresponding to their relative stability: Machaerocereus gummosus (Pitahaya agria) > Cholla opuntia (Cholla) > Lophocereu schottii (Garambullo). Thermodynamic properties of proteins and their changes upon denaturation (temperature of denaturation, enthalphy, and the number of ruptured hydrogen bonds) were correlated with the secondary structure of proteins and disappearance of alpha-helix.

  5. Pitaya (Stenocereus spp. , cactaceae): An ancient and modern fruit crop of Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Pimienta-Barrios, E. (Universidad de Guadalajara, Guadalajara (Mexico)); Nobel, P.S (Univ. of California, Los Angeles, CA (United States))

    Pitayas from various species were an important edible fruit in semiarid lands of tropical and subtropical Mexico in ancient times. Recently, farmers have been cultivating plants selected from the wild, such as Stenocereus queretaroensis in the Sayula Basin of Jalisco. These cacti can flower and produce fruit before the onset of the summer rainy period. Their fruits have an attractively colored pulp (often dark red) with digestible seeds and without the nasty glochids found on cactus pears. The sugar content is 10 to 11%. The shelf life is only a few days, as the fruits tend to dehisce longitudinally. Pitayas bring a competitive price in local markets, resulting in a substantial financial return with relatively low inputs of water, fertilizer, and pesticides.

  6. Does Ferocactus wislizeni (Cactaceae) have a between-year seed bank?

    Science.gov (United States)

    Bowers, Janice E.

    2000-01-01

    Field and laboratory experiments at Tumamoc Hill, Tucson, Arizona, U.S.A., demonstrated that Ferocactus wislizeni, a common perennial cactus in the northern Sonoran Desert, has a between-year seed bank. In laboratory studies, F. wislizeni seeds lost dormancy during storage at room temperature and had a light requirement for germination. Field experiments suggested that as much as 2% of the annual seed crop might escape post-dispersal predation even when unprotected; where suitable safe sites exist, a higher percentage might escape. Germination of seed recovered monthly from above- and below-ground components of an artificial seed bank showed that seeds can survive at least 18 months in and on the soil. Seed banks enable F. wislizeni to take advantage of favorable rains and temperatures throughout the growing season, thus increasing the number of opportunities for germination. Moreover, seed banks enable F. wislizeni to respond hugely when the climate seems especially favorable, thus producing the large cohorts necessary to compensate for high seedling mortality. (C) 2000 Academic Press.

  7. Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae).

    Science.gov (United States)

    Bonatelli, Isabel A S; Carstens, Bryan C; Moraes, Evandro M

    2015-01-01

    Microsatellite markers (also known as SSRs, Simple Sequence Repeats) are widely used in plant science and are among the most informative molecular markers for population genetic investigations, but the development of such markers presents substantial challenges. In this report, we discuss how next generation sequencing can replace the cloning, Sanger sequencing, identification of polymorphic loci, and testing cross-amplification that were previously required to develop microsatellites. We report the development of a large set of microsatellite markers for five species of the Neotropical cactus genus Pilosocereus using a restriction-site-associated DNA sequencing (RAD-seq) on a Roche 454 platform. We identified an average of 165 microsatellites per individual, with the absolute numbers across individuals proportional to the sequence reads obtained per individual. Frequency distribution of the repeat units was similar in the five species, with shorter motifs such as di- and trinucleotide being the most abundant repeats. In addition, we provide 72 microsatellites that could be potentially amplified in the sampled species and 22 polymorphic microsatellites validated in two populations of the species Pilosocereus machrisii. Although low coverage sequencing among individuals was observed for most of the loci, which we suggest to be more related to the nature of the microsatellite markers and the possible bias inserted by the restriction enzymes than to the genome size, our work demonstrates that an NGS approach is an efficient method to isolate multispecies microsatellites even in non-model organisms.

  8. Regeneration of Carnegiea gigantea (Cactaceae) since 1850 in three populations in the northern Sonoran Desert

    Science.gov (United States)

    Drezner, Taly Dawn

    2006-03-01

    Saguaro cacti ( Carnegiea gigantea) are long-lived and exhibit great variability in growth that makes age estimation problematic. A few single-site studies have focused on those locales where long-term data (e.g. 85 years) are available. Using a newly developed technique, 733 saguaros were sampled in three locales (Silverbell, Harcuvar, Kofa) across Arizona and their age structure reconstructed for the last 150 years based on a mathematical model of the heights of individuals. This is the first study to compare regeneration at multiple locations across the species' range. Regression analysis for each site (years and frequency of individuals established during that year) was run and residuals extracted to determine peaks and troughs in regeneration over time. Correlation was run on the residuals between sites, and chi-square analysis was employed to compare frequency of good and bad regeneration years between Kofa and Silverbell. Peaks and troughs represent regeneration as well as survivorship and mortality. Several large cohorts established at Kofa and Harcuvar since 1850, while at Silverbell well over 80% of sampled saguaros established after the late 1930s. This more recent recruitment at Silverbell may be related to the major freezing event of 1937 whose impact was likely greater at the cooler Silverbell site. Despite the widely different population structures at Silverbell and Kofa, recruitment trends in both populations were statistically linked as both locales often benefited from the same favorable periods for regeneration ( P < 0.001). The Harcuvar population shares some common peaks and troughs in regeneration over time with Kofa and Silverbell, but its relationship to them is not statistically significant. Some trends overlap in some locales, such as the favorable regeneration period in the late 1800s and early 1900s, particularly at Silverbell and Kofa as well as at other known sites. However, each population has its own signature. Silverbell is a youthful population likely shaped by severe freezing events, while Kofa has many individuals representing regeneration at several different periods. Regeneration, mortality and subsequent population structure is shaped by both regional-scale influences as well as more localized conditions over the long and short terms.

  9. Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae.

    Directory of Open Access Journals (Sweden)

    Isabel A S Bonatelli

    Full Text Available Microsatellite markers (also known as SSRs, Simple Sequence Repeats are widely used in plant science and are among the most informative molecular markers for population genetic investigations, but the development of such markers presents substantial challenges. In this report, we discuss how next generation sequencing can replace the cloning, Sanger sequencing, identification of polymorphic loci, and testing cross-amplification that were previously required to develop microsatellites. We report the development of a large set of microsatellite markers for five species of the Neotropical cactus genus Pilosocereus using a restriction-site-associated DNA sequencing (RAD-seq on a Roche 454 platform. We identified an average of 165 microsatellites per individual, with the absolute numbers across individuals proportional to the sequence reads obtained per individual. Frequency distribution of the repeat units was similar in the five species, with shorter motifs such as di- and trinucleotide being the most abundant repeats. In addition, we provide 72 microsatellites that could be potentially amplified in the sampled species and 22 polymorphic microsatellites validated in two populations of the species Pilosocereus machrisii. Although low coverage sequencing among individuals was observed for most of the loci, which we suggest to be more related to the nature of the microsatellite markers and the possible bias inserted by the restriction enzymes than to the genome size, our work demonstrates that an NGS approach is an efficient method to isolate multispecies microsatellites even in non-model organisms.

  10. [Cardon dato (Stenocereus griseus, Cactaceae) fruit pulp as raw material for marmalade production].

    Science.gov (United States)

    Emaldi, Unai; Nassar, Jafet M; Semprum, Carla

    2006-03-01

    This study aimed to examine the possibility of producing marmalades from the fruit pulp of the red and white varieties of card6n dato (Stenocereus griseus). We developed the formulations of the marmalades and evaluated their stability during three months of storage at room temperature. As first step, we characterized the fruits of the two color varieties, observing that despite the considerable difference between both color varieties, there were no significant differences in average weight, dimensions and proportion of pulp, skin and seeds. The pulp of the two color types had high pH (5.2) values and low contents of reductor sugars (3.59 g/ 100 g white variety 2.23 g/ 100 g red variety), non reductor sugars (0.75 g/ 100 g white variety and 2.03 g/ 100 g red variety), pectin (0.14 g/ 100 g white variety and 0.23 g/ 100 g red variety) and acids (7.67 g/ 100 g white variety and 0.15 g/ 100 g red variety). It is needed to include sugar, pectin, and citric acid in the marmalade formula. The first marmalades produced were gummy, a problem that we solved adding the acid from the beginning of the making process. During the three months of storage, the marmalades had good acceptance by the evaluators, this despite slight fluctuations observed in pH, solid contents, and acidity.

  11. La Historia de una especie del patrimonio holguinero: Escobaria cubensis (Cactaceae "el cactus enano". Primera parte

    Directory of Open Access Journals (Sweden)

    Alena Reyes Fornet

    2010-01-01

    Full Text Available Presenta una investigación sobre la historia taxonómica y estudios sobre la ecología del "cactus enano de Holguín", Escobaria cubensis (Britton & Rose Hunt, 1978 desde 1909 hasta 1990, elementos importantes, para la conservación y como contribución al conocimiento de la historia del patrimonio natural holguinero, con datos históricos sobre visitas de personalidades del mundo y Holguín. Se estudiaron 37 materiales correspondientes a notas de viajes, cartas y publicaciones. Se señala la posibilidad de que el colector, J. Shafer estuvo en dos zonas de distribución. Las contribuciones al conocimiento de su ecología hasta 1990 se basaron en la observación y descripción.

  12. Nonlinear models applied to seed germination of Rhipsalis cereuscula Haw (Cactaceae

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    Terezinha Aparecida Guedes

    2014-09-01

    Full Text Available The objective of this analysis was to fit germination data of Rhipsalis cereuscula Haw seeds to the Weibull model with three parameters using Frequentist and Bayesian methods. Five parameterizations were compared using the Bayesian analysis to fit a prior distribution. The parameter estimates from the Frequentist method were similar to the Bayesian responses considering the following non-informative a priori distribution for the parameter vectors: gamma (10³, 10³ in the model M1, normal (0, 106 in the model M2, uniform (0, Lsup in the model M3, exp (μ in the model M4 and Lnormal (μ, 106 in the model M5. However, to achieve the convergence in the models M4 and M5, we applied the μ from the estimates of the Frequentist approach. The best models fitted by the Bayesian method were the M1 and M3. The adequacy of these models was based on the advantages over the Frequentist method such as the reduced computational efforts and the possibility of comparison.

  13. Notes on the Cactaceae of Curaçao, Aruba, Bonaire and North Venezuela

    NARCIS (Netherlands)

    Wagenaar Hummelinck, P.

    1938-01-01

    La flora de cactos de las islas situadas entre Trinidad y la península de La Goajira comprende trece especies silvestres, aparte del género Melocactus: Pereskia Guamacho, Opuntia caribaea, O. curassavica, O. Wentiana, O. elatior, Rhipsalis cassutha, Hylocereus Lemairei, Acanthocereus pentagonus, Lem

  14. Survival dynamics of Melocactus conoideus Buining & Brederoo (Cactaceae, a threatened species endemic to northeastern Brazil

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    Hévila Prates Luz-Freire

    2014-06-01

    Full Text Available Studies on the survival of species are essential to understanding their biology and to developing effective conservation and management plans. This study aimed to determine the best model to explain the survival of the species Melocactus conoideus on the basis of time, density, age structure and habitat location, as well as to describe the interactions among those factors. The study was conducted in three M. conoideus habitat patches in the municipality of Vitória da Conquista, in the state of Bahia, Brazil, only one of which was within a "conservation unit" (protected area. In each patch, we selected 120 specimens of M. conoideus, which were marked with identification plates and classified by developmental stage and density. The survival of those individuals was monitored for a period of one year. The overall survival of M. conoideus was 87.5% and was found to correlate with the month, as well as with the interaction between the factors Patch and Density. Our results show that the survival of M. conoideus individuals is related to the intrinsic characteristics of each habitat patch and suggest that more areas should be set aside for the conservation of this species.

  15. Seed size and photoblastism in species belonging to tribe Cacteae (Cactaceae).

    Science.gov (United States)

    Rojas-Aréchiga, Mariana; Mandujano, María C; Golubov, Jordan K

    2013-05-01

    The response of seed germination towards light and the relationship to seed traits has been studied particularly well in tropical forests. Several authors have shown a clear adaptive response of seed size and photoblastism, however, the evolutionary significance of this relationship for species inhabiting arid environments has not been fully understood and only some studies have considered the response in a phylogenetic context. We collected seeds from 54 cacti species spread throughout the tribe Cacteae to test whether there was correlated evolution of photoblastism, seed traits and germination using a reconstructed phylogeny of the tribe. For each species we determined the photoblastic response under controlled conditions, and seed traits, and analyzed the results using phylogenetically independent contrasts. All studied species were positive photoblastic contrasting with the basal Pereskia suggesting an early evolution of this trait. Seeds from basal species were mostly medium-sized, diverging into two groups. Seeds tend to get smaller and lighter suggesting an evolution to smaller sizes. No evidence exists of a relationship between seed size and photoblastic response suggesting that the photoblastic response within members of this tribe is not adaptive though it is phylogenetically fixed and that is coupled with environmental cues that fine tune the germination response.

  16. Polymorphic microsatellite markers for the rare and endangered cactus Uebelmannia pectinifera (Cactaceae) and its congeneric species.

    Science.gov (United States)

    Moraes, E M; Cidade, F W; Silva, G A R; Machado, M C

    2014-12-04

    The cactus genus Uebelmannia includes 3 narrow endemic species associated with rocky savanna habitats in eastern South America. Because of their rarity and illegal over-collection, all of these species are endangered. Taxonomic uncertainties resulting from dramatic local variation in morphology within Uebelmannia species preclude effective conservation efforts, such as the reintroduction or translocation of plants, to restore declining populations. In this study, we developed and characterized 18 perfect, dinucleotide simple-sequence repeat markers for U. pectinifera, the most widely distributed species in the genus, and tested the cross-amplification of these markers in the remaining congeneric species and subspecies. All markers were polymorphic in a sample from 2 U. pectinifera populations. The effective number of alleles ranged from 1.6 to 8.7, with an average per population of 3.3 (SE ± 0.30) and 4.5 (SE ± 0.50). Expected heterozygosity ranged from 0.375 to 0.847 and 8-10 loci showed departures from Hardy- Weinberg equilibrium in the analyzed populations. Based on the observed polymorphism level of each marker, as well as the analysis of null allele presence and evidence of amplification of duplicate loci, a subset of 12 loci can be used as reliable markers to investigate the genetic structure, diversity, and species limits of the Uebelmannia genus.

  17. Anatomical and morphological features of seedlings of some Cactoideae Eaton (Cactaceae Juss. species

    Directory of Open Access Journals (Sweden)

    Halyna Kalashnyk

    2016-12-01

    Full Text Available Three-month-old seedlings of 11 species of the subfamily Cactoideae (Melocactus bahiensis, Melocactus curvispinus, Echinopsis eyriesii, E. mirablis, E. peruviana, Oreocereus celsianus, Rebutia flavistyla, Rebutia minuscula, Astrophytum myriostigma, Mamillaria columbiana, and M. prolifera have been studied. These plants exhibit a uniseriate epidermis, covered by a thin cuticle. Except for E. peruviana and A. myriostigma, no hypodermis could be detected. The shoots of all studied specimens consist mainly of cortex parenchyma with large thin-walled cells. The pith parenchyma is composed of much smaller cells. Due to the fact that the cortex parenchyma comprises the largest portion of the cross-sectional area, it can be concluded that it is the main water-storing tissue. The extent of vascular tissue development varies. Collateral vascular bundles are present in the stele. The studied seedlings contain various ergastic substances, in particular inclusions of calcium oxalate (all studied species, starch (Mammillaria prolifera, E. mirabilis, and the genus Melocactus, inulin-like inclusions, and occasionally lipid drops (some Echinopsis species. Thus, it was found that all studied plants have a highly specialized anatomical and morphological structure. At the same time, the epidermis and hypodermis are poorly developed. Accordingly, the adaptation to arid conditions of the examined seedlings involves an increased growth of the water-storing tissue and the production of ergastic substances.

  18. Reproductive biology of Echinopsis terscheckii (Cactaceae): the role of nocturnal and diurnal pollinators.

    Science.gov (United States)

    Ortega-Baes, P; Saravia, M; Sühring, S; Godínez-Alvarez, H; Zamar, M

    2011-01-01

    The aim of this study was to analyse the reproductive biology of Echinopsis terscheckii, a species endemic to northwest Argentina that has nocturnal flowers. We expected that this species had a generalised pollination system, with moths and diurnal visitors as the primary pollinators. To test this, we studied the floral biology, breeding system and floral visitors of this species and the effectiveness of nocturnal and diurnal visitors. Floral biology was defined based on floral morphology, floral cycle and nectar production of the flowers. The breeding system and relative contributions of diurnal and nocturnal visitors to fruit and seed set were analysed through field experiments. E. terscheckii flowers opened at sunset and closed the following day. The peak of nectar production occurred at midnight. Flowers were determined to be self-incompatible. Moths, bees and birds were identified as floral visitors. Moths were the most frequent visitors at night, whereas bees were the most frequent visitors during the day. Fruit production by diurnal pollinators was less than that by nocturnal pollinators; among all floral visitors, moths were the most effective pollinators. We have demonstrated for the first time that moths are the primary pollinators of columnar cacti of the genus Echinopsis. Our results suggest that moths might be important pollinators of columnar cactus species with nocturnal flowers in the extra-tropical deserts of South America.

  19. MORFOLOGÍA POLÍNICA DE ESPECIES ARGENTINAS DE REBUTIA S. STR. (CACTACEAE, CACTOIDEAE

    Directory of Open Access Journals (Sweden)

    Silvina Garralla

    2008-01-01

    subsp. wessneriana y R. padcayensis con la finalidad de aportar datos que permitan la delimitación de estas especies. El material polínico procede de ejemplares coleccionados en distintas localidades montanas del noroeste argentino. Las observaciones, conteos y mediciones de los granos de polen se realizaron con microscopio óptico (MO y microscopio electrónico de barrido (MEB. El estudio de estos taxones pone de manifiesto que Rebutia es un género estenopolínico; los granos son apolares o isopolares, radiosimétricos, de tamaño mediano (32-55 um, ámbito circular a subcircular; tricolpados o pantocolpados; la exina es tectada, perforada, nanoequinada. Se discuten diferencias entre los taxones y se brinda una clave palinológica para su diferenciación.

  20. Distribution and habitat in Mexico of Dactylopius Costa (Hemiptera: Dactylopiidae) and their cacti hosts (Cactaceae: Opuntioideae).

    Science.gov (United States)

    Chávez-Moreno, C K; Tecante, A; Casas, A; Claps, L E

    2011-01-01

    The distribution pattern of species of the genus Dactylopius Costa in Mexico was analyzed in relation to the distribution of their host plants (subfamily Opuntioideae) to evaluate the specificity of the insect-host association. The distribution of Dactylopius currently recognized is narrower than that of its hosts and probably is not representative. Therefore, a broader distribution of the Dactylopius species in correspondence with those of their hosts was hypothesized. Insects and their hosts were collected and georeferenced in 14 states of Mexico from 2005 to 2007. The distribution areas, maps, and habitat characteristics of Dactylopius, Opuntia sensu stricto, Nopalea and Cylindropuntia were determined on the basis of field collections and examination of museum collections. This information was complemented with information from the exhaustive examination of microscope slides from a local insect collection, plants from local herbaria, and literature reviews. The current distribution of the genus Dactylopius and its hosts included 22 and 25 states of Mexico, respectively, and Dactylopius had a continuous distribution according to its hosts, broader than recognized hitherto. The new georeferenced records of the five Mexican Dactylopius species are reported. Insects with morphological characteristics of D. confusus combined with those of D. salmianus were identified, as well as insects with characteristics of D. opuntiae combined with those of D. salmianus. These records suggest that the number of local Dactylopius species could be higher than previously thought or that possible new processes of hybridization between native and introduced species may be occurring.

  1. Implications of seed size for seedling survival in Carnegiea gigantea and Ferocactus wislizeni (Cactaceae)

    Science.gov (United States)

    Bowers, Janice E.; Pierson, E.A.

    2001-01-01

    Larger seeds have been shown to convey benefits for seedling survival but the mechanisms of this process are not well understood. In this study, seed size and seedling survival were compared for 2 sympatric cactus species, Carnegiea gigantea (Engelm.) Britt. & Rose and Ferocactus wislizeni (Engelm.) Britt. & Rose, in laboratory and field experiments in the northern Sonoran Desert. Both species have small seeds, but Ferocactus seeds are nearly twice as long and 3 times as heavy as those of Carnegiea. The difference in size is perpetuated after germination: new Ferocactus seedlings have 4 times the estimated volume of new Carnegiea seedlings. In an outdoor experiment, annual survivorship of both species was low but was 6 times higher for Ferocactus (6 seedlings, 8.1%) than Carnegiea (1 seedling, 1.4%). The pattern of seedling mortality in relation to temperature and rain suggests that, after the initial flush of seed and seedling predation, drought and heat took a greater toll on Carnegiea than Ferocactus seedlings, probably because the larger seedling volume of Ferocactus conferred greater drought tolerance. In addition, F. wislizeni could become established without benefit of nurse plants whereas C. gigantea could not; this might reflect differential tolerance to high soil temperatures.

  2. Generative period in development of Mammillaria Haw. (Cactaceae Juss. plants in greenhouse conditions

    Directory of Open Access Journals (Sweden)

    Kateryna Baglay

    2013-04-01

    Full Text Available The taxonomic composition of the collection of the genus Mammillariain O.V. Fomin Botanical Garden, as well as the peculiarities of blooming and seed germination have been considered. The list of the plants from the genus Mammillariain this collection which are included into IUCN Red List have been represented. The optimal conditions of seed conservation have been determined.

  3. Factors affecting establishment success of the endangered Caribbean cactus Harrisia portoricensis (Cactaceae).

    Science.gov (United States)

    Rojas-Sandoval, Julissa; Meléndez-Ackerman, Elvia

    2012-06-01

    Early plant stages may be the most vulnerable within the life cycle of plants especially in arid ecosystems. Interference from exotic species may exacerbate this condition. We evaluated germination, seedling survival and growth in the endangered Caribbean cactus Harrisia portoricensis, as a function of sunlight exposure (i.e., growing under open and shaded areas), different shade providers (i.e., growing under two native shrubs and one exotic grass species), two levels of predation (i.e., exclusion and non-exclusion) and variable microenvironmental conditions (i.e., temperature, PAR, humidity). Field experiments demonstrated that suitable conditions for germination and establishment of H. portoricensis seedling are optimal in shaded areas beneath the canopy of established species, but experiments also demonstrated that the identity of the shade provider can have a significant influence on the outcome of these processes. Harrisia portoricensis seedlings had higher probabilities of survival and grew better (i.e., larger diameters) when they were transplanted beneath the canopy of native shrubs, than beneath the exotic grass species, where temperature and solar radiation values were on average much higher than those obtained under the canopies of native shrubs. We also detected that exclusion from potential predators did not increase seedling survival. Our combined results for H. portoricensis suggested that the modification of microenvironmental conditions by the exotic grass may lower the probability of recruitment and establishment of this endangered cactus species.

  4. Agrobiodiversity of cactus pear (Opuntia, Cactaceae in the Meridional Highlands Plateau of Mexico

    Directory of Open Access Journals (Sweden)

    Juan Antonio Reyes-Agüero

    2011-08-01

    Full Text Available Mexico is characterized by a remarkable richness of Opuntia, mostly at the Meridional Highlands Plateau; it is also here where the greatest richness of Opuntia variants occurs. Most of these variants have been maintained in homegardens; however, the gathering process which originated these homegardens has been disrupted over the past decades, as a result of social change and the destruction of large wild nopaleras. If the variants still surviving in homegardens are lost, these will be hard to recover, that is, the millenary cultural heritage from the human groups that populated the Mexican Meridional Highland Plateau will be lost forever. This situation motivated the preparation of a catalogue that records the diversity of wild and cultivated Opuntia variants living in the meridional Highlands Plateau. To this end, 379 samples were obtained in 29 localities, between 1998 and 2003. The information was processed through Twinspan. All specimens were identified and preserved in herbaria. Botanical keys and descriptions were elaborated. The catalogue includes information on 126 variants comprising 18 species. There were species with only one variant (Opuntia atropes, O. cochinera, O. jaliscana, O. leucotricha, O. rzedowskii and O. velutina, two (O. durangensis, O. lindheimeri, O. phaeacantha and O. robusta, five (O. joconostle and O. lasiacantha, seven (O. chavena, 12 (O. hyptiacantha and O. streptacantha, 15 (O. ficus-indica, 22 (O. albicarpa, and up to 34 (O. megacantha. Additionally, 267 common cactus pear names were related to those variants.

  5. Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes.

    Science.gov (United States)

    Lone, A B; Colombo, R C; Andrade, B L G; Takahashi, L S A; Faria, R T

    2016-06-01

    The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

  6. Characterization of calcium oxalate biominerals in some (non-Cactaceae) succulent plant species.

    Science.gov (United States)

    Monje, Paula V; Baran, Enrique J

    2010-01-01

    The water-accumulating leaves of crassulacean acid metabolism plants belonging to five different families were investigated for the presence of biominerals by infrared spectroscopic and microscopic analyses. Spectroscopic results revealed that the mineral present in succulent species of Agavaceae, Aizoaceae, and Asphodelaceae was calcium oxalate monohydrate (whewellite, CaC2O4 x H2O). Crystals were predominantly found as raphides or solitary crystals of various morphologies. However, representative Crassulaceae members and a succulent species of Asteraceae did not show the presence of biominerals. Overall, these results suggest no correlation between calcium oxalate generation and crassulacean acid metabolism in succulent plants.

  7. Reproductive ability and possibilities of restoration of the genus Parodia Speg. (Cactaceae Juss. in greenhouses conditions

    Directory of Open Access Journals (Sweden)

    Kateryna Baglay

    2015-05-01

    Full Text Available The collection of the genus Рarodia of the O.V. Fomin Botanical Garden of the Taras Shevchenko Kyiv National University covers 74 species and varieties. About 60% of representatives of the genus pass through the full development cycle (plants form flowers and fruits. The fruits are formed as a result of self-fertilization that is important moment in greenhouses conditions. The collection of the genus Рarodia was analyzed on presence of rare and endangered species, protected by Convention of International Trade of Endangered Species of Wild Fauna and Flora (CITES and Red List of the International Union for Conservation of Nature and Natural Resources (IUCN. Their categorization by rarity has been done. The germination of fresh gathered seed was studied. The species, which biennial seedlings are recommended to the use in phytodesign have been selected.

  8. Variation in the local population dynamics of the short-lived Opuntia macrorhiza (Cactaceae).

    Science.gov (United States)

    Haridas, C V; Keeler, Kathleen H; Tenhumberg, Brigitte

    2015-03-01

    Spatiotemporal variation in demographic rates can have profound effects for population persistence, especially for dispersal-limited species living in fragmented landscapes. Long-term studies of plants in such habitats help with understanding the impacts of fragmentation on population persistence but such studies are rare. In this work, we reanalyzed demographic data from seven years of the short-lived cactus Opuntia macrorhiza var. macrorhiza at five plots in Boulder, Colorado. Previous work combining data from all years and all plots predicted a stable population (deterministic log lamda approximately 0). This approach assumed that all five plots were part of a single population. Since the plots were located in a suburban-agricultural interface separated by highways, grazing lands, and other barriers, and O. macrorhiza is likely dispersal limited, we analyzed the dynamics of each plot separately using stochastic matrix models assuming each plot represented a separate population. We found that the stochastic population growth rate log lamdaS varied widely between populations (log lamdaS = 0.1497, 0.0774, -0.0230, -0.2576, -0.4989). The three populations with the highest growth rates were located close together in space, while the two most isolated populations had the lowest growth rates suggesting that dispersal between populations is critical for the population viability of O. macrorhiza. With one exception, both our prospective (stochastic elasticity) and retrospective (stochastic life table response experiments) analysis suggested that means of stasis and growth, especially of smaller plants, were most important for population growth rate. This is surprising because recruitment is typically the most important vital rate in a short-lived species such as O. macrorhiza. We found that elasticity to the variance was mostly negligible, suggesting that O. macrorhiza populations are buffered against large temporal variation. Finally, single-year elasticities to means of transitions to the smallest stage (mostly due to reproduction) and growth differed considerably from their long-term elasticities. It is important to be aware of this difference when using models to predict the effect of manipulating plant vital rates within the time frame of typical plant demographic studies.

  9. Genomic relations among 31 species of Mammillaria haworth (Cactaceae) using random amplified polymorphic DNA.

    Science.gov (United States)

    Mattagajasingh, Ilwola; Mukherjee, Arup Kumar; Das, Premananda

    2006-01-01

    Thirty-one species of Mammillaria were selected to study the molecular phylogeny using random amplified polymorphic DNA (RAPD) markers. High amount of mucilage (gelling polysaccharides) present in Mammillaria was a major obstacle in isolating good quality genomic DNA. The CTAB (cetyl trimethyl ammonium bromide) method was modified to obtain good quality genomic DNA. Twenty-two random decamer primers resulted in 621 bands, all of which were polymorphic. The similarity matrix value varied from 0.109 to 0.622 indicating wide variability among the studied species. The dendrogram obtained from the unweighted pair group method using arithmetic averages (UPGMA) analysis revealed that some of the species did not follow the conventional classification. The present work shows the usefulness of RAPD markers for genetic characterization to establish phylogenetic relations among Mammillaria species.

  10. Physical characterization of Rhipsalis (Cactaceae fruits and seeds germination in different temperatures and light regimes

    Directory of Open Access Journals (Sweden)

    A. B. Lone

    Full Text Available Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

  11. Development of microsatellite markers using next-generation sequencing for the columnar cactus Echinopsis chiloensis (Cactaceae).

    Science.gov (United States)

    Ossa, Carmen G; Larridon, Isabel; Peralta, Gioconda; Asselman, Pieter; Pérez, Fernanda

    2016-12-01

    The aim of this study was to develop microsatellite markers as a tool to study population structure, genetic diversity and effective population size of Echinopsis chiloensis, an endemic cactus from arid and semiarid regions of Central Chile. We developed 12 polymorphic microsatellite markers for E. chiloensis using next-generation sequencing and tested them in 60 individuals from six sites, covering all the latitudinal range of this species. The number of alleles per locus ranged from 3 to 8, while the observed (Ho) and expected (He) heterozygosity ranged from 0.0 to 0.80 and from 0.10 to 0.76, respectively. We also detected significant differences between sites, with FST values ranging from 0.05 to 0.29. Microsatellite markers will enable us to estimate genetic diversity and population structure of E. chiloensis in future ecological and phylogeographic studies.

  12. Molecular phylogeny of tribe Rhipsalideae (Cactaceae) and taxonomic implications for Schlumbergera and Hatiora.

    Science.gov (United States)

    Calvente, Alice; Zappi, Daniela C; Forest, Félix; Lohmann, Lúcia G

    2011-03-01

    Tribe Rhipsalideae is composed of unusual epiphytic or lithophytic cacti that inhabit humid tropical and subtropical forests. Members of this tribe present a reduced vegetative body, a specialized adventitious root system, usually spineless areoles and flowers and fruits reduced in size. Despite the debate surrounding the classification of Rhipsalideae, no studies have ever attempted to reconstruct phylogenetic relationships among its members or to test the monophyly of its genera using DNA sequence data; all classifications formerly proposed for this tribe have only employed morphological data. In this study, we reconstruct the phylogeny of Rhipsalideae using plastid (trnQ-rps16, rpl32-trnL, psbA-trnH) and nuclear (ITS) markers to evaluate the classifications previously proposed for the group. We also examine morphological features traditionally used to delimit genera within Rhipsalideae in light of the resulting phylogenetic trees. In total new sequences for 35 species of Rhipsalideae were produced (out of 55; 63%). The molecular phylogeny obtained comprises four main clades supporting the recognition of genera Lepismium, Rhipsalis, Hatiora and Schlumbergera. The evidence gathered indicate that a broader genus Schlumbergera, including Hatiora subg. Rhipsalidopsis, should be recognized. Consistent morphological characters rather than homoplastic features are used in order to establish a more coherent and practical classification for the group. Nomenclatural changes and a key for the identification of the genera currently included in Rhipsalideae are provided.

  13. Anatomical and morphological spine variation in Gymnocalycium kieslingii subsp. castaneum (Cactaceae)

    Science.gov (United States)

    Gebauer, Roman; Řepka, Radomír; Šmudla, Radek; Mamoňová, Miroslava; Ďurkovič, Jaroslav

    2016-01-01

    Abstract Although spine variation within cacti species or populations is assumed to be large, the minimum sample size of different spine anatomical and morphological traits required for species description is less studied. There are studies where only 2 spines were used for taxonomical comparison amnog species. Therefore, the spine structure variation within areoles and individuals of one population of Gymnocalycium kieslingii subsp. castaneum (Ferrari) Slaba was analyzed. Fifteen plants were selected and from each plant one areole from the basal, middle and upper part of the plant body was sampled. A scanning electron microscopy was used for spine surface description and a light microscopy for measurements of spine width, thickness, cross-section area, fiber diameter and fiber cell wall thickness. The spine surface was more visible and damaged less in the upper part of the plant body than in the basal part. Large spine and fiber differences were found between upper and lower parts of the plant body, but also within single areoles. In general, the examined traits in the upper part had by 8–17% higher values than in the lower parts. The variation of spine and fiber traits within areoles was lower than the differences between individuals. The minimum sample size was largely influenced by the studied spine and fiber traits, ranging from 1 to 70 spines. The results provide pioneer information useful in spine sample collection in the field for taxonomical, biomechanical and structural studies. Nevertheless, similar studies should be carried out for other cacti species to make generalizations. The large spine and fiber variation within areoles observed in our study indicates a very complex spine morphogenesis. PMID:27698579

  14. An open door for illegal trade: online sale of Strombocactus disciformis (Cactaceae

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    Vania R. Olmos-Lau

    2016-09-01

    Full Text Available Online trade of CITES listed species has become a persistent threat which is difficult to measure and control. The use of online markets is growing day by the day as technology becomes more available and familiar to people of all ages and interests. Species trade can now be propagated remotely hardly without any real human interaction. We develop a quick-easy method to assess the online availability of the genus Strombocactus, a highly collectible cactus, to understand the real magnitude of this new form of threat and the possible menace it could be for these Mexican cacti. We used the Google.com site to do an online search in four languages (Spanish, English, French and German for the offer of adult plants or seeds. We found specimens and seeds available in major online markets like ebay, amazon, cactusplaza.com and mercado libre. Plant price range from €10.00 to €30.00 plus shipping and handling. The plants were also offered in local online stores in countries like the USA, France, Germany, Australia, Czech Republic, the United Kingdom, and others; some sellers claim they have no obstacles for “shipping across countries” and others openly declare the natural localities where seeds were extracted. Only a minority of these online stores openly stated that the cacti were obtained from CITES registered nurseries or that the cacti were grown through propagules or seeds. Our method is easily transferable to estimate the illegal market for any species. There is an active online trade of Strombocactus species and other species listed in CITES without the necessary documentation. Compliance or other regulation mechanisms are needed in order to promote species conservation.

  15. THE GENERATIVE REPRODUCTIVE CHARACTERISTICS OF RED DRAGON FRUIT (Hylocereus.polyrhizus (Web.) Britton & Rose, CACTACEAE)

    OpenAIRE

    Eniek Kriswiyanti

    2013-01-01

    This study aimed to investigate the generative reproductive characteristics of red dragon fruit (Hylocereus polyrhizus (Web.) Britton & Rose), that is the characteristics and the development of male and female reproductive system and the embryo. The flowering morphology was observed through the gametogenesis process and the process of embryogenesis was observed using the methods of squash, cytolysis, and embedding of flower before anthesis, during and after anthesis. The results showed th...

  16. THE GENERATIVE REPRODUCTIVE CHARACTERISTICS OF RED DRAGON FRUIT (Hylocereus.polyrhizus (Web. Britton & Rose, CACTACEAE

    Directory of Open Access Journals (Sweden)

    Eniek Kriswiyanti

    2013-04-01

    Full Text Available This study aimed to investigate the generative reproductive characteristics of red dragon fruit (Hylocereus polyrhizus (Web. Britton & Rose, that is the characteristics and the development of male and female reproductive system and the embryo. The flowering morphology was observed through the gametogenesis process and the process of embryogenesis was observed using the methods of squash, cytolysis, and embedding of flower before anthesis, during and after anthesis. The results showed that the flower funnelled form with many calyxes, stamens and crowns. The pollen was circular, sulcus, trilate, reticulate, and spheroid. The development of microgametophyte was at the 2nd stages with three nucleuses and the pistil developed after anthesis.  Anthesis was taken place at night time, fertilization and pollen developed before anthesis and has not germinating. A single pistil, the head of pistil with many branches, the style longer than the stamen, open type.  Seeds anathrophus, endosperm and embryo with the globular shaped, developed 5 days after anthesis, the embryo torpedo likes and the differentiation of primer tissues seen in the seeds 7 days after anthesis, and arilus generated from development of megasporangium. Keywords: embedding, self incompatibility, sferoidal, anatrophus, arillus.

  17. Leaf and Stem CO2 Uptake in the Three Subfamilies of the Cactaceae 1

    Science.gov (United States)

    Nobel, Park S.; Hartsock, Terry L.

    1986-01-01

    Net CO2 uptake over 24-hour periods was examined for the leaves and for the stems of 11 species of cacti representing all three subfamilies. For Pereskia aculeata, Pereskia grandifolia, and Maihuenia poeppigii (subfamily Pereskioideae), all the net shoot CO2 uptake was by the leaves and during the daytime. In contrast, for the leafless species Carnegiea gigantea, Ferocactus acanthodes, Coryphantha vivipara, and Mammillaria dioica (subfamily Cactoideae), all the shoot net CO2 uptake was by the stems and at night. Similarly, for leafless Opuntia ficus-indica (subfamily Opuntioideae), all net CO2 uptake occurred at night. For leafy members of the Opuntioideae (Pereskiopsis porteri, Quiabentia chacoensis, Austrocylindropuntia subulata), at least 88% of the shoot CO2 uptake over 24 hours was by the leaves and some CO2 uptake occurred at night. Leaves responded to the instantaneous level of photosynthetically active radiation (PAR) during the daytime, as occurs for C3 plants, whereas nocturnal CO2 uptake by stems of O. ficus-indica and F. acanthodes responded to the total daily PAR, as occurs for Crassulacean acid metabolism (CAM) plants. Thus, under the well-watered conditions employed, the Pereskioideae behaved as C3 plants, the Cactoideae behaved as CAM plants, and the Opuntioideae exhibited characteristics of both pathways. PMID:16664741

  18. Morphological variation and the process of domestication of Stenocereus stellatus (Cactaceae) in Central Mexico.

    Science.gov (United States)

    Casas, A; Caballero, J; Valiente-Banuet, A; Soriano, J A; Dávila, P

    1999-04-01

    Morphological variation was analyzed in wild, managed in situ, and cultivated populations of the columnar cactus Stenocereus stellatus in central Mexico. The purpose was to evaluate whether morphological divergence between manipulated and wild populations has resulted from domestication processes. Variation of 23 morphological characters was analyzed among 324 individuals from 19 populations of the Tehuacán Valley and La Mixteca Baja. Multivariate statistical analyses were used to group individuals and populations according to their morphological similarity. Individuals grouped according to the way of management and fruit characteristics were the most relevant for grouping. Within each region, sweet fruits with pulp colors other than red were more frequent in cultivated populations, where fruits were also larger, contained more and bigger seeds, and had thinner peel and fewer spines than fruits from wild individuals. Phenotypes common in managed in situ and cultivated populations generally occur in the wild but in lower frequencies. Artificial selection has thus operated by enhancing and maintaining desirable rare phenotypes in managed in situ and cultivated populations, causing divergent patterns of morphological variation from wild populations. Cultivation has caused the strongest level of divergence, but divergence has also been significant with management of wild populations in situ.

  19. Phylogenetic origins of Lophocereus (Cactaceae) and the senita cactus-senita moth pollination mutualism.

    Science.gov (United States)

    Hartmann, Stefanie; Nason, John D; Bhattacharya, Debashish

    2002-07-01

    Recent ecological research has revealed that the Sonoran Desert columnar cactus Lophocereus and the pyralid moth Upiga virescens form an obligate pollination mutualism, a rare but important case of coevolution. To investigate the phylogenetic origins of this unusual pollination system, we used molecular sequence data to reconstruct the phylogeny of the four taxa within the genus Lophocereus and to determine the phylogenetic position of Lophocereus within the North American columnar cacti (tribe Pachycereeae). Our analysis included Lophocereus, six Pachycereus species, Carnegiea gigantea, and Neobuxbaumia tetetzo within the subtribe Pachycereinae, and Stenocereus thurberi as an outgroup within the Stenocereinae. Extensive screening of chloroplast and mitochondrial genomes failed to reveal sequence variation within Lophocereus. At a deeper phylogenetic level, however, we found strong support for the placement of Lophocereus within Pachycereus as sister group to the hummingbird-pollinated P. marginatus. We discuss possible hypotheses that may explain the transition from bat pollination (ancestral) to moth and hummingbird pollination in Lophocereus and P. marginatus, respectively. Additional phylogenetic analyses suggest that the genus Pachycereus should be expanded to include Lophocereus, Carnegiea, Neobuxbaumia, and perhaps other species, whereas P. hollianus may need to be excluded from this clade. Future study will be needed to test taxonomic distinctions within Lophocereus, to test for parallel cladogenesis between phylogroups within Lophocereus and Upiga, and to fully delineate the genus Pachycereus and relationships among genera in the Pachycereinae.

  20. Managing diversity: Domestication and gene flow in Stenocereus stellatus Riccob. (Cactaceae) in Mexico

    Science.gov (United States)

    Cruse-Sanders, Jennifer M; Parker, Kathleen C; Friar, Elizabeth A; Huang, Daisie I; Mashayekhi, Saeideh; Prince, Linda M; Otero-Arnaiz, Adriana; Casas, Alejandro

    2013-01-01

    Microsatellite markers (N = 5) were developed for analysis of genetic variation in 15 populations of the columnar cactus Stenocereus stellatus, managed under traditional agriculture practices in central Mexico. Microsatellite diversity was analyzed within and among populations, between geographic regions, and among population management types to provide detailed insight into historical gene flow rates and population dynamics associated with domestication. Our results corroborate a greater diversity in populations managed by farmers compared with wild ones (HE = 0.64 vs. 0.55), but with regional variation between populations among regions. Although farmers propagated S. stellatus vegetatively in home gardens to diversify their stock, asexual recruitment also occurred naturally in populations where more marginal conditions have limited sexual recruitment, resulting in lower genetic diversity. Therefore, a clear-cut relationship between the occurrence of asexual recruitment and genetic diversity was not evident. Two managed populations adjacent to towns were identified as major sources of gene movement in each sampled region, with significant migration to distant as well as nearby populations. Coupled with the absence of significant bottlenecks, this suggests a mechanism for promoting genetic diversity in managed populations through long distance gene exchange. Cultivation of S. stellatus in close proximity to wild populations has led to complex patterns of genetic variation across the landscape that reflects the interaction of natural and cultural processes. As molecular markers become available for nontraditional crops and novel analysis techniques allow us to detect and evaluate patterns of genetic diversity, genetic studies provide valuable insights into managing crop genetic resources into the future against a backdrop of global change. Traditional agriculture systems play an important role in maintaining genetic diversity for plant species. PMID:23762520

  1. Evolution of Polaskia chichipe (Cactaceae) under domestication in the Tehuacan Valley, central Mexico: reproductive biology.

    Science.gov (United States)

    Otero-Arnaiz, Adriana; Casas, Alejandro; Bartolo, Carmen; Pérez-Negrón, Edgar; Valiente-Banuet, Alfonso

    2003-04-01

    Polaskia chichipe, a columnar cactus, is cultivated for its edible fruits in central Mexico. This study analyzed whether artificial selection has modified its reproduction patterns and caused barriers to pollen exchange between wild, managed in situ, and cultivated populations. Anthesis was diurnal (∼16 h in winter, ∼10 h in spring) as well as partly nocturnal (∼12 h in winter, ∼3 h in spring), and flowers were pollinated by bees, hummingbirds, and hawk moths. Manual cross-pollination was ∼37-49% effective in all populations. Self-pollination was ∼12% successful in the wild, but twice as successful (∼22-27%) in managed and cultivated populations. Diurnal pollination was ∼35-55% effective in winter and 100% in spring. Nocturnal pollination was successful only in winter (15%). Crosses among individuals were more effective within populations than among populations, including populations under a similar management regimen. The least successful crosses were between wild and cultivated populations. Flowers were produced in all populations from January to March, but flowering peaks differed by 1 mo among wild, managed, and cultivated populations and by 2 mo between wild and cultivated populations. The latter interrupted pollen exchange in May. Seeds from managed and cultivated populations germinated faster than those from wild individuals. Domestication has seemingly favored self-compatible P. chichipe plants with higher fruit yield, a longer period of fruit production, and faster seed germination, attributes that have resulted in partial reproductive barriers between wild and manipulated populations.

  2. The origins of an important cactus crop, Opuntia ficus-indica (Cactaceae): new molecular evidence.

    Science.gov (United States)

    Griffith, M Patrick

    2004-11-01

    Opuntia ficus-indica is a long-domesticated cactus crop that is important in agricultural economies throughout arid and semiarid parts of the world. The biogeographic and evolutionary origins of this species have been obscured through ancient and widespread cultivation and naturalization. The origin of O. ficus-indica is investigated through the use of Bayesian phylogenetic analyses of nrITS DNA sequences. These analyses support the following hypotheses: that O. ficus-indica is a close relative of a group of arborescent, fleshy-fruited prickly pears from central and southern Mexico; that the center of domestication for this species is in central Mexico; and that the taxonomic concept of O. ficus-indica may include clones derived from multiple lineages and therefore be polyphyletic.

  3. Systematyc notes and a Detailed description of Opuntia ficus-indica (L) Mill. (CACTACEAE)

    OpenAIRE

    J. Antonio Reyes-Agüero; J. Rogelio Aguirre-Rivera; Hernández, Héctor M.

    2005-01-01

    Opuntia ficus-indica is the cactus species with the highest economic importance worldwide. It is cultivated for its fruits, forage, or as host of the cochineal insect, but only in México their young cladodes are consumed as vegetables. The main goals of this study were: a) to integrate systematic notes about its common nomenclature, origin, reproductive biology and ploidy levels of O. ficus-indica; b) to present a complete botanical description based on specimens collected in north-central Mé...

  4. Biomechanics and anatomy of cladode junctions for two Opuntia (Cactaceae) species and their hybrid.

    Science.gov (United States)

    Bobich, E G; Nobel, P S

    2001-03-01

    Hybridization between the introduced arborescent Opuntia ficus-indica and the native shrubby O. littoralis has led to populations, referred to as O. "occidentalis," which form thickets that can dominate hillsides of chaparral and that can survive fires. Because the thickets apparently develop via vegetative reproduction, O. "occidentalis" was hypothesized to have a greater ability than its parent species to reproduce vegetatively due to weaker cladode junctions. Of the three taxa, the junctions for O. "occidentalis" had the least amount of wood, despite having cladode masses and junction cross-sectional areas similar to those of O. littoralis. The cladodes of O. "occidentalis" resisted deflection about their junctions the least and their junctions required the least amount of applied mass and the smallest bending moment to fail mechanically. The junction wood for all three taxa consisted mostly of parenchyma, with lesser amounts of cells with thickened secondary cell walls, indicating that some junction strength depended on hydrostatic pressure, especially for terminal junctions. Libriform fibers, which contribute to support and resist bending moments, were about 80% less frequent in the sub-subterminal junctions of O. "occidentalis" than in O. ficus-indica and O. littoralis. Vascular tracheids, which probably reduced shear among cells in the wood, were 90% less frequent in the terminal and sub-subterminal junction wood of O. "occidentalis" compared to O. littoralis. Thus wood characteristics can account for the weaker junctions of O. "occidentalis" compared to those of O. ficus-indica and O. littoralis, which apparently increases the ability of the hybrid to reproduce vegetatively.

  5. Leaf and Stem CO(2) Uptake in the Three Subfamilies of the Cactaceae.

    Science.gov (United States)

    Nobel, P S; Hartsock, T L

    1986-04-01

    Net CO(2) uptake over 24-hour periods was examined for the leaves and for the stems of 11 species of cacti representing all three subfamilies. For Pereskia aculeata, Pereskia grandifolia, and Maihuenia poeppigii (subfamily Pereskioideae), all the net shoot CO(2) uptake was by the leaves and during the daytime. In contrast, for the leafless species Carnegiea gigantea, Ferocactus acanthodes, Coryphantha vivipara, and Mammillaria dioica (subfamily Cactoideae), all the shoot net CO(2) uptake was by the stems and at night. Similarly, for leafless Opuntia ficus-indica (subfamily Opuntioideae), all net CO(2) uptake occurred at night. For leafy members of the Opuntioideae (Pereskiopsis porteri, Quiabentia chacoensis, Austrocylindropuntia subulata), at least 88% of the shoot CO(2) uptake over 24 hours was by the leaves and some CO(2) uptake occurred at night. Leaves responded to the instantaneous level of photosynthetically active radiation (PAR) during the daytime, as occurs for C(3) plants, whereas nocturnal CO(2) uptake by stems of O. ficus-indica and F. acanthodes responded to the total daily PAR, as occurs for Crassulacean acid metabolism (CAM) plants. Thus, under the well-watered conditions employed, the Pereskioideae behaved as C(3) plants, the Cactoideae behaved as CAM plants, and the Opuntioideae exhibited characteristics of both pathways.

  6. 75 FR 35746 - Endangered and Threatened Wildlife and Plants; 90-Day Finding on a Petition to List the Honduran...

    Science.gov (United States)

    2010-06-23

    ... (herbaceous and woody species), Cactaceae (cactus species), and Euphorbiaceae (herbs, shrubs, trees, and some... are food sources for this hummingbird: Nopalea hondurensis (paddle cactus, in the Cactaceae family), Stenocereus yunckeri (NCN, in the Cactaceae family), Pilosocereus leucocephalus (NCN, in the Cactaceae...

  7. Características físicas, químicas e organolépticas de quipá (Tacinga inamoena, Cactaceae Physical chemical and chemicalcharacteristics of 'quipá' (Tacinga inamoena, Cactaceae

    Directory of Open Access Journals (Sweden)

    Andréa Carla Mendonça de Souza

    2007-08-01

    Full Text Available A Opuntia ficus-indica tem-se destacado como principal produtora de frutos e forragens, motivo pelo qual tem sido bastante pesquisada. Além desta espécie, no semi-árido do Nordeste é encontrada a Tacinga inamoena, planta nativa, cujo fruto, embora também utilizado pelo agricultor como alternativa alimentar, não foi objeto de nenhuma pesquisa até o momento, justificando este trabalho para avaliar seu potencial nutricional e industrial, por meio das características organolépticas, químicas e físicas, conteúdo nutricional e composição mineral. O quipá apresenta características organolépticas similares às de frutos de mesmo gênero, com rendimento da porção comestível, polpa e pericarpo carnoso de 62,87% do peso total do fruto. No que diz respeito à composição química, a polpa difere significativamente do pericarpo carnoso, com superioridade deste último, que apresenta maior teor de minerais, destacando-se dentre estes o cálcio, o magnésio e o potássio que apresentaram valores de 587,04mg, 257,02mg e 318,01mg, respectivamente. Os resultados evidenciam que o quipá é adequado para consumo in natura e apresenta potencial para aproveitamento industrial.Provided that the Opuntia fícus-indica is the main producer of fruit and forages, it has been the object of extensive research. Besides, another species Tacinga inamoena, native plant, which its fruit has been used as an alternative food source can also be found in the semi-arid northeastern region of Brazil. However, this native plant had not been the object of any research yet. The aim of this work is to evaluate its industrial and nutritional potential through the organoleptic, physical, chemical characteristics, nutritional content and mineral composition. The Quípa shows organoleptic characteristics similar to other fruit of the same gender. Its edible portion, pulp and fleshy pericarp, corresponds to 62, 8% of the total fruit weight. As regard to chemical composition, the pulp differs significantly from the fleshy pericarp, the latter showing higher content of minerals among them calcium, magnesium and potassium with values of 587,04mg, 257,02mg and 318,01mg, respectively. The results showed that Quípa is appropriate for consumption in natura and shows industrial potential.

  8. Caracterização citogenética em Schlumbergera truncata (Haworth Moran e Schlumbergera × buckleyi (T. Moore Tjaden (Cactaceae Cytogenetic characterization of Schlumbergera truncata (Haworth Moran and Schlumbergera × buckleyi (T. Moore Tjaden (Cactaceae

    Directory of Open Access Journals (Sweden)

    Flavia Aparecida Ortolani

    2007-06-01

    Full Text Available O número cromossômico diplóide de Schlumbergera truncata e Schlumbergera x buckleyi, de indivíduos com diferentes tipos de coloração de pétalas, foi determinado usando-se pontas de raízes. A utilização de 8-hidroxiquinoleína 0,003 M à 36 °C por 3 horas possibilitou melhor separação cromossômica. Técnica de bandeamento C e de coloração Giemsa permitiram o estudo cariológico dessas espécies. O híbrido Schlumbergera × buckleyi (rósea apresenta 2n = 22 cromossomos com fórmula cariotípica 16 M + 6 SM. Schlumbergera truncata, apresentando pétalas nas cores vermelha, branca e pink, possui 2n = 22 cromossomos, formulação cariotípica idêntica à de Schlumbergera × buckleyi, enquanto a planta com flores de coloração amarelada mostrou 2n = 34 cromossomos. A classificação cromossômica foi baseada no índice centromérico. Nas plantas que apresentam coloração vermelha, branca, pink e rósea nas pétalas, o melhor período de obtenção de metáfases corresponde ao período de florescimento. Schlumbergera truncata com flores amareladas apresenta dois picos anuais de divisão mitótica. Esses resultados dão suporte à um melhor entendimento da biologia no gênero Schlumbergera e auxiliam na classificação taxonômica nos casos onde apenas as características fenotípicas não são suficientemente confiáveis para a classificação das plantas no mesmo táxon.The diploid chromosome number of Schlumbergera truncata and Schlumbergera × buckleyi, in individuals with different types of petal color, was determined using root tips. The use of 8-hydroxyquinolein 0.003 M at 36 ºC provided better chromosome separation. C-banding technique and Giemsa coloration allowed the karyological study of these species. Schlumbergera × buckleyi hybrid (light pink species has 2n = 22 chromosomes with karyotype formula 16M + 6SM. Schlumbergera truncata with red, white, and pink petals and 2n = 22 chromosomes has karyotype formula identical to Schlumbergera × buckleyi, while the plant with yellowish flowers has 2n = 34 chromosomes. Chromosome classification was based on the centromeric index. In plants with white, red, pink and light pink petal color, the best time to obtain metaphases is during flowering. Schlumbergera truncata with yellowish flowers has two annual peaks of mitotic division. These results give us a better understanding of the biology of the genus Schlumbergera and aid in taxonomic classification where phenotypic characteristics alone are not reliable enough to classify plants of the same taxon.

  9. Una especie nueva de Strombocactus (Cactaceae del río Moctezuma, Querétaro, México A new species of Strombocactus (Cactaceae from Moctezuma River, Querétaro, Mexico

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    Salvador Arias

    2010-12-01

    Full Text Available Se describe e ilustra Strombocactus corregidorae S. Arias et E. Sánchez, nueva especie del cañón del Infiernillo, en el río Moctezuma, en los límites del estado de Querétaro con Hidalgo. Difiere de S. disciformis por presentar cuerpos más prominentes, con espinas de mayor tamaño, rígidas, gruesas, persistentes y de color negro grisáceo, flores amarillas, así como semillas con células de paredes periclinales planas, microrrelieve finamente reticulado y la región hilo-micropilar no está cubierta por un estrofíolo. Se incluye una clave taxonómica para la identificación de las especies de Strombocactus , con base en caracteres del tallo, flor y la semilla.Strombocactus corregidorae S. Arias et E. Sanchez, from the Infiernillo Canyon, in the Moctezuma River, at the border between the Mexican states of Querétaro and Hidalgo, is described and illustrated. It differs from S. disciformis by its massive body, larger, strong, thick, persistent, black-gray spines, yellow flower, seeds with flat periclinal wall cells, finely reticulate micro-relief, and hilum-micropylar region not covered by a strophiole. An identification key to Strombocactus species, based on stem, flower, and seed characters is included.

  10. Uso, manejo y conservacion de “yosú” Stenocereus griseus (Cactaceae,en la alta guajira colombiana Usage, Managment and Conservation of “yosú”, Stenocereus griseus (Cactaceae, in the Upper Guajira, Colombia

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    Melo Sandra

    2007-04-01

    Full Text Available En este trabajo se caracteriza el sistema de uso, manejo, demanda y oferta potencial de Stenocereus griseus, un cactus columnar muy utilizado por la comunidad indígena Wayúu en la Guajira, Caribe colombiano. Actualmente el uso de S. griseus se restringe a la recolección de madera seca ("yotojoro" para construcción de viviendas, la recolección de frutos para la alimentación, de tallos jóvenes para alimentos de cabras y para utilizarlos en cercas vivas. Una evaluación de los factores que afectan las poblaciones permitió determinar cinco categorías de daño: las ocasionadas por corte con machete (tipo I, insectos (tipo II, cabras (tipo III, aves (tipo IV y viento (tipo V. La demanda anual de "yotojoro" está sujeta a la necesidad de construcción de los habitantes del área, que es definida como ocasional. El método actual de cosecha y extracción del mismo no está generando desequilibrio local en el mantenimiento de la especie. La demanda de cardones para cercas vivas y pastoreo es constante durante todo el año y es la que genera el mayor impacto de daño. Finalmente, se discuten los principales problemas de conservación de la especie y se recomienda establecer alternativas de manejo para las actividades de pastoreo.The present paper characterizes the usage and handling system, demand and potential offer of Stenocereus griseus, a columnar cactus widely used by the "Wayúu", an indigenous community in the Colombian Caribbean region of "La Guajira". The current use of S. griseus is restricted to the collection of dry timber wood ("yotojoro" for house building; of the fruit, which is used for human consumption; and of the young stems, used as goat fodder, and as living fences. An evaluation of the factors that affect the populations allowed the detection of five different harm categories: those caused by cuttings with cutlass (type I; by insects (type II; goats (type III; birds
    (type IV; and wind (type V. The annual "yotojoro" demand is subject to the building needs of the people in the area, which is defined as occasional. The current cropping and extraction method was not found to be causing any local disequilibrium in the species population  maintenance. There is a steady cardon demand for fodder and living fences all year long; one which is causing the highest harm impact. Finally, the main conservation problems of the species are discussed, together with the suggestion of management alternatives for grazing activities.

  11. USO, MANEJO Y CONSERVACIÓN DE “yosú”, Stenocereus griseus (CACTACEAE, EN LA ALTA GUAJIRA COLOMBIANA Usage, Managment and Conservation of “yosú”, Stenocereus griseus (Cactaceae, in the Upper Guajira, Colombia

    Directory of Open Access Journals (Sweden)

    SORAYA VILLALOBOS

    2007-06-01

    Full Text Available En este trabajo se caracteriza el sistema de uso, manejo, demanda y oferta potencial de Stenocereus griseus, un cactus columnar muy utilizado por la comunidad indígena Wayúu en la Guajira , Caribe colombiano. Actualmente el uso de S. griseus se restringe a la recolección de madera seca (“yotojoro” para construcción de viviendas, la recolección de frutos para la alimentación, de tallos jóvenes para alimentos de cabras y para utilizarlos en cercas vivas. Una evaluación de los factores que afectan las poblaciones permitió determinar cinco categorías de daño: las ocasionadas por corte con machete (tipo I, insectos (tipo II, cabras (tipo III, aves (tipo IV y viento (tipo V. La demanda anual de “yotojoro” está sujeta a la necesidad de construcción de los habitantes del área, que es definida como ocasional. El método actual de cosecha y extracción del mismo no está generando desequilibrio local en el mantenimiento de la especie. La demanda de cardones para cercas vivas y pastoreo es constante durante todo el año y es la que genera el mayor impacto de daño. Finalmente, se discuten los principales problemas de conservación de la especie y se recomienda establecer alternativas de manejo para las actividades de pastoreo.The present paper characterizes the usage and handling system, demand and potential offer of Stenocereus griseus, a columnar cactus widely used by the “Wayúu”, an indigenous community in the Colombian Caribbean region of “ La Guajira ”. The current use of S. griseus is restricted to the collection of dry timber wood (“yotojoro” for house building; of the fruit, which is used for human consumption; and of the young stems, used as goat fodder, and as living fences. An evaluation of the factors that affect the populations allowed the detection of five different harm categories: those caused by cuttings with cutlass (type I; by insects (type II; goats (type III; birds (type IV; and wind (type V. The annual “yotojoro” demand is subject to the building needs of the people in the area, which is defined as occasional. The current cropping and extraction method was not found to be causing any local disequilibrium in the species population maintenance. There is a steady cardon demand for fodder and living fences all year long; one which is causing the highest harm impact. Finally, the main conservation problems of the species are discussed, together with the suggestion of management alternatives for grazing activities.

  12. Specialization clines in the pollination systems of agaves (Agavaceae) and columnar cacti (Cactaceae): A phylogenetically controlled meta-analysis.

    Science.gov (United States)

    Munguía-Rosas, Miguel A; Sosa, Vinicio J; Ojeda, Mario M; De-Nova, J Arturo

    2009-10-01

    The biogeography of plant-animal interactions is a novel topic on which many disciplines converge (e.g., reproductive biology, biogeography, and evolutionary biology). Narrative reviews have indicated that tropical columnar cacti and agaves have highly specialized pollination systems, while extratropical species have generalized systems. However, this dichotomy has never been quantitatively tested. We tested this hypothesis using traditional and phylogenetically informed meta-analysis. Three effect sizes were estimated from the literature: diurnal, nocturnal, and hand cross-pollination (an indicator of pollen limitation). Columnar cactus pollination systems ranged from purely bat-pollinated in the tropics to generalized pollination, with diurnal visitors as effective as nocturnal visitors in extratropical regions; even when phylogenetic relatedness among species is taken into account. Metaregressions identified a latitudinal increase in pollen limitation in columnar cacti, but this increase was not significant after correcting for phylogeny. The currently available data for agaves do not support any latitudinal trend. Nectar production of columnar cacti varied with latitude. Although this variation is positively correlated with pollination by diurnal visitors, it is influenced by phylogeny. The degree of specificity in the pollination systems of columnar cacti is heavily influenced by ecological factors and has a predictable geographic pattern.

  13. Essential oils composition of two Sicilian cultivars of Opuntia ficus-indica (L.) Mill. (Cactaceae) fruits (prickly pear).

    Science.gov (United States)

    Zito, Pietro; Sajeva, Maurizio; Bruno, Maurizio; Rosselli, Sergio; Maggio, Antonella; Senatore, Felice

    2013-01-01

    The essential oils composition of the skin, pulp and seeds from fruits of two Sicilian cultivars of Opuntia ficus-indica (cv. Sanguigna and cv. Surfarina) has been obtained by hydrodistillation and the possible antioxidant, antimicrobial and semiochemical roles have been investigated comparing the data with those reported in the literature. The presence of antioxidants and antimicrobials found in this study increases the spectrum of compounds that have beneficial properties in O. ficus-indica. In addition, several compounds identified in this study have been reported to influence the behaviour of Ceratitis capitata, a phytophagous pest which causes severe damages to several crops including O. ficus-indica and the kairomonal activity of the odour of the fruits seems provided by a blend of compounds found in the various matrices analysed.

  14. An integrated management plan for the control of Opuntia stricta (Cactaceae in the Kruger National Park, South Africa

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    W.D. Lotter

    1998-01-01

    Full Text Available Opuntia stricta is the most problematic alien plant species in the Kruger National Park (KNP where 30000 ha around Skukuza have been invaded by the weed. Control of 0. stricta is expensive and time consuming and there are insufficient resources available for the task at present. Biological control using the moth Cactoblastis cactorum has only been partially successful and herbicidal control remains the main method for tackling the problem. In order to optimise the control operation against 0. stricta, a management plan has been developed for the KNP. The infested region of the park has been divided into 18 management units, each of which will be treated in turn. During the first three years the control operations will clear 0. stricta from the peripheral units, to prevent the spread of the weed into other areas of the KNP, after which the central units will be tackled. The objective is to destroy all mature fruiting plants and those nearing maturity to curb long-range (seed dispersal of the weed. The small juvenile plants, which are difficult to detect, will be suppressed by C. cactorum. After five years the problem should be contained to the extent that an ongoing 'holding' programme will be sufficient to keep the weed in check. The success of the plan will depend on increased resources being committed to alien plant control in the Kruger National Park.

  15. Primary characterization and evaluation of anti ulcerogenic activity of an aqueous extract from callus culture of Cereus peruvianus Mill. (Cactaceae).

    Science.gov (United States)

    Jayme, Milena O; Ames, Franciele Q; Bersani-Amado, Ciomar A; Machado, Maria de Fatima P S; Mangolin, Claudete A; Goncalves, Regina A C; de Oliveira, Arildo J B

    2015-01-01

    In the current study we reported cultivation, extraction procedure, analysis and preliminary characterization of the aqueous extract from Cereus peruvianus callus culture and evaluated its anti ulcerogenic activity in vivo models of experimental ulcers in Wistar rats. The obtained aqueous extract from callus (AC) was dialyzed and subjected to freeze-thaw process, providing a possible polysaccharide. The carbohydrate and protein contents of the aqueous extract were estimated at 53.4% and 0.66%, respectively, composed primarily of galactose, arabinose and galacturonic acid, with minor amounts of glucose. This appeared heterogeneous when analyzed by high-performance size-exclusion chromatography and a multiangle laser light scattering detector (HPSEC-MALLS). The AC was found to be significantly effective against ethanol-induced lesions but was ineffective against indomethacin-induced lesions. The callus culture of C. peruvianus is an alternative source for the synthesis of substances originally produced by plants. The calluses grown indefinitely in vitro under controlled conditions are stable tissues, and the aqueous extract from calluses may be used instead of fully developed plants using the protocols described in this study.

  16. Frugivory and seed dispersal by birds in Cereus jamacaru DC. ssp. jamacaru (Cactaceae) in the Caatinga of Northeastern Brazil.

    Science.gov (United States)

    Gomes, V G N; Quirino, Z G M; Araujo, H F P

    2014-02-01

    Studies of the dispersal modes of plants aid our understanding of the dynamics of resource and its availability for dispersal agents. The present work sought to characterize the fruiting patterns of the native Caatinga (dryland) cactus Cereus jamacaru, identify its principal dispersers, and evaluate the effects of seed passage through digestive tract of dispersers on its germination. Cereus jamacaru present an annual fruiting pattern and fruiting peaks occurred during June/2009 and February/2010. A total of 135 visits by nine species of resident Caatinga bird species were recorded. The most frequent visiting bird species were Paroaria dominicana and Euphonia chlorotica. Length of bird visits varied from 15 seconds to 4 minutes and seeds removed by birds travelled 10.6 ± 11.2 m until dispersers make the first landing perch, in some cases more than 40 meters away. Germination tests show birds had a high quantity of viable seeds of C. jamacaru in its feces. Seeds that passed through the digestive tract of birds showed a similar germinability of the seeds of the control group. However, the seeds dispersed by birds showed lowest mean germination time related to the control group seeds. This study highlights the potential role of birds as seed dispersers of C. jamacaru, swallowing the whole seeds and defecating intact seeds, accelerating the germination process and transporting seeds away from the mother plant.

  17. Genetic variation and evolution of Polaskia chichipe (Cactaceae) under domestication in the Tehuacán Valley, central Mexico.

    Science.gov (United States)

    Otero-Arnaiz, Adriana; Casas, Alejandro; Hamrick, James L; Cruse-Sanders, Jennifer

    2005-05-01

    Polaskia chichipe is a columnar cactus under artificial selection in central Mexico because of its edible fruits. Our study explored the effect of human manipulation on levels and distribution of genetic variation in wild, silviculturally managed and cultivated sympatric populations. Total genetic variation, estimated in nine populations with five microsatellite loci, was H(T) = 0.658 +/- 0.026 SE, which was mainly distributed within populations (H(S) = 0.646) with low differentiation among them (F(ST) = 0.015). Fixation index (F(IS)) in all populations was positive, indicating a deficit of heterozygous individuals with respect to Hardy-Weinberg expectations. When populations were pooled by management type, the highest expected heterozygosity (H(E) = 0.631 +/- 0.031 SE) and the lowest fixation index (F(IS) = 0.07) were observed in wild populations, followed by cultivated populations (H(E) = 0.56 +/- 0.03 SE, F(IS) = 0.14), whereas the lowest variation was found in silviculturally managed populations (H(E) = 0.51 +/- 0.05 SE, F(IS) = 0.17). Low differentiation among populations under different management types (F(ST) 0.005, P < 0.04) was observed. A pattern of migration among neighbouring populations, suggested from isolation by distance (r2 = 0.314, P < 0.01), may have contributed to homogenizing populations and counteracting the effects of artificial selection. P. chichipe, used and managed for at least 700 generations, shows morphological differentiation, changes in breeding system and seed germination patterns associated with human management, with only slight genetic differences detected by neutral markers.

  18. Collection of plants of the genus Melocactus (Tourn. Link et Otto (Cactaceae Juss. and peculiarities of their reproduction

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    Kateryna Baglay

    2014-04-01

    Full Text Available Taxonomic composition of Melocactus collection at O.V. Fomin Botanical Garden, as well as peculiarities of their reproduction under the conditions of introduction has been considered. Rare and threatened Melocactus species in the collection were identified.

  19. Germination response of Hylocereus setaceus (Salm-Dyck ex DC: ) Ralf Bauer (Cactaceae) seeds to temperature and reduced water potentials.

    Science.gov (United States)

    Simão, E; Takaki, M; Cardoso, V J M

    2010-02-01

    The germination response of Hylocereus setaceus seeds to isothermic incubation at different water potentials was analysed by using the thermal time and hydrotime models, aiming to describe some germination parameters of the population and to test the validity of the models to describe the response of the seeds to temperature and water potential. Hylocereus setaceus seeds germinated relatively well in a wide range of temperatures and the germination was rate limited from 11 to 20 degrees C interval and beyond 30 degrees C until 40 degrees C, in which the germination rate respectively shifts positively and negatively with temperature. The minimum or base temperature (T(b)) for the germination of H. setaceus was 7 degrees C, and the ceiling temperature varied nearly from 43.5 to 59 degrees C depending on the percent fraction, with median set on 49.8 degrees C. The number of degrees day necessary for 50% of the seeds to germinate in the infra-optimum temperature range was 39.3 degrees C day, whereas at the supra-optimum interval the value of theta = 77 was assumed to be constant throughout. Germination was sensitive to decreasing values of psi in the medium, and both the germinability and the germination rate shift negatively with the reduction of psi, but the rate of reduction changed with temperature. The values of base water potential (psi(b)) shift to zero with increasing temperatures and such variation reflects in the relatively greater effect of low psi on germination in supra optimum range of T. In general, the model described better the germination time courses at lower than at higher water potentials. The analysis also suggest that Tb may not be independent of psi and that psi(b(g)) may change as a function of temperature at the infra-otimum temperature range.

  20. Cuantificación fisicoquímica en gorro turco [Melocactus zehntneri (Britton & Rose Luetzelburg - Cactaceae

    Directory of Open Access Journals (Sweden)

    E.N. NUNES

    2016-03-01

    Full Text Available RESUMO No Nordeste do Brasil é bastante comum e variado o uso da coroa-de-frade (Melocactus spp.. Assim, o objetivo deste estudo foi realizar diversas análises em cladódios de coroa-de-frade [Melocactus zehntneri (Britton & Rose Luetzelburg], para que possamos compreender as razões estes usos. Os cladódios foram coletados na cidade de Campina Grande e levados ao Laboratório de Engenharia de Alimentos da Universidade Federal de Campina Grande, onde foram feitas as seguintes análises: peso, altura, diâmetro, cor, umidade, acidez, pH, ácido ascórbico, carotenoides, flavonoides e compostos fenólicos, tanto na epiderme quanto no parênquima aquífero. Os resultados mostraram que a epiderme tem uma acidez elevada e mais ácido ascórbico, e um pH mais baixo em comparação com o parênquima aquífero. O conteúdo de carotenoides, flavonoides e compostos fenólicos, foram diferentes nas partes estudadas. A epiderme apresentou sempre maiores quantidades de carotenoides, flavonoides e compostos fenólicos em relação ao parênquima aquífero, nas três plantas estudadas, indicando que a maior parte da defesa da planta acontece neste tecido. Mais estudos são necessários com técnicas mais precisas, para identificar os componentes bioativos desta espécie.

  1. Report of Six Cases Cured by Extenal Application of Cactaceae%仙人掌外敷验案六则

    Institute of Scientific and Technical Information of China (English)

    王保民

    2001-01-01

    @@ 仙人掌为仙人掌科植物仙人掌的全株,它不仅是一种观赏的花卉,还是一味很好的中药.取仙人掌地上部分若干,洗净去针刺,捣烂如泥,加食醋或明矾少许备用.治疗时视病情将配制好的仙人掌敷于患处,每日1换或半日1换,并将浸有生理盐水的消毒敷料敷于其上,以减少摩擦和蒸发.笔者在临床上使用仙人掌外敷治疗许多疾病,取得了很好的效果,现介绍如下.

  2. LARVICIDAL ACTIVITY OF PERESKIA BLEO (KUNTH) DC. (CACTACEAE) FRUIT ENDOCARP CRUDE AND FRACTIONATED EXTRACTS AGAINST AEDES AEGYPTI (L.) (DIPTERA: CULICIDAE).

    Science.gov (United States)

    Thongwat, Damrongpan; Ganranoo, Lucksagoon; Chokchaisiri, Ratchanaporn

    2014-11-01

    The use of insecticides can cause adverse effects in vector control, a plant bio-insecticide is an advantageous substitute. Currently, the promising mosquito larvicidal activity from plant extracts has been reported worldwide, including Thailand. In this study, the endocarp of Pereskia bleo (Kunth) DC. fruit was extracted with distilled water and ethanol. Crudes and fractionated groups of the extracts were evaluated for their larvicidal efficacy against the 3rd instar larvae of Aedes aegypti. At 48 hours of exposure, it was found that the activities of the extracts were higher than 24-hour's. The ethanolic extracts showed stronger activities than the aqueous ones, indicating the lower LC50 values of both crude and fractionated group extracts. The most toxic activity was found in a fractionated group of the ethanolic extract, E-Gr3, with significantly lowest LC50 values of 707.94 and 223.12 ppm for 24- and 48-hour detection times, respectively. The bioassay results indicated the larvicidal property against the Ae. aegypti mosquito of the P. bleo plant extracts. A safety for non-target organisms or an action on other mosquito vectors of this plant, should be further investigated.

  3. Aspectos de la Biología reproductiva de una población de Wigginsia vorwerckiana (Cactaceae

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    Amaya Márquez Marisol

    2000-06-01

    Full Text Available RESUMENWigginsia vorwerckiana(Werdermann D.M. Porter es una especie que está distribuida porColombia, Argentina, Uruguay y Brasil. En Colombia se encuentra en las zonas secas, una deellas el enclave subxerifítico de la Sabana de Bogotá a 2.700m. Este es un ecosistema pocoestudiado y actualmente se encuentra amenazado por procesos antrópicos. Allí se encuentraWigginsia vorwerckiana, una cactácea de hábito globoso que crece enterrada en el suelo con unadistribución agregada. La floración duró todo el tiempo de estudio (diciembre 1998 enero2000. Las flores crecen en una estructura denominada cefalio y se producen de maneracentrípeta. Son de color amarillo, hermafroditas y 65.42% de la población presenta herco-gamia. La antesis de las flores es diurna, abriendo en la mañana y cerrando en la noche. Laduración media de las flores es dos días. Las flores no producen néctar y la recompensa queofrecen a sus polinizadores es polen el que no producen en gran cantidad. W. vorwerckianarecibe visitas de tres especies de Lepidópteros, una especie de Díptero y una especie deHimenóptero. Sólo esta última Lasioglossum sp. (Halictidae muestra una alta fidelidad floralsiendo responsable de 85% de las visitas que recibe este cacto y actúa como polinizadoreficiente. Por lo anterior podemos decir que W. vorwerckianaes una especie oligotrópica,mientras que Lasioglossumes oligoléctico pero polifílico, ya que además de polinizar a este cacto,visita otras especies de cactáceas y algunas especies de compuestas presentes en la zona. Lasvisitas de Lasioglossumocurren de las 10 a las 15 horas con la mayor frecuencia de visitación entrelas 12 y 13 horas, cuando la apertura de las flores es máxima, y la receptividad y disponibilidadde polen son mayores. Tanto la frecuencia de visitación como la apertura de las flores estáninfluenciadas por la luz y la temperatura, siendo mayores en las horas soleadas y con tem-peraturas superiores a los 20°C. Una vez las flores son polinizadas y fecundadas, requieren enpromedio de cuatro meses para su maduración. Los frutos son consumidos por hormigasdel género Solenopsis(Formicidae quienes hacen la dispersión de las semillas. W. vorwerckianaes una especie autocompatible ya que es capaz de formar frutos con semillas producto de laautopolinización y en ausencia de su polinizador; sin embargo, esta especie requiere de losservicios especializados de esta abeja para efectuar la polinización cruzada y mantener un buennivel de variabilidad genética en la población. Todo lo anterior muestra la importancia de conservar la zona de estudio, ya que es una zona no protegida en la que el disturbio pone enriesgo la existencia de esta población de cactos, de la abeja que funciona como su polinizador yde los animales que consumen sus frutos.

  4. The germination of seeds of Epiphyllum phyllanthus (L.) Haw. (Cactaceae) is controlled by phytochrome and by nonphytochrome related process

    OpenAIRE

    Simão, Edson [UNESP; Nakamura, Adriana Tiemi [UNESP; Takaki,Massanori

    2010-01-01

    As sementes de Epiphyllum phyllanthus apresentam alta sensibilidade à luz e a sua germinação pode ser promovida pela luz verde de segurança por meio da resposta de fluência muito baixa mediada pelo fitocromo A. Parte da população de sementes tem fitocromo B na forma ativa (Fve) suficiente para promover a germinação no escuro. Sementes de Epiphyllum phyllanthus germinam em uma ampla faixa de temperatura de 10 a 40°C, atingindo germinação completa na faixa de 15 a 30°C. Acima de 35°C a velocida...

  5. The epiphytic Cactaceae Hylocereus setaceus (Salm-Dick ex DC. ralf bauer seed germination is controlled by light and temperature

    Directory of Open Access Journals (Sweden)

    Edson Simão

    2007-07-01

    Full Text Available The effect of light and temperature on seed germination in Hylocereus setaceus was analyzed by isothermic incubations under continuous white light and darkness. The minimum temperature for germination was between 5 to 10°C and the maximum between 45 to 50°C. The optimum temperaturewais between 25 and 30°C. The involvement of phytochrome, by very low fluence response, in controlled seed germination was attained by incubation in a gradient of photoequilibrium of phytochrome. The results indicated that seeds of H. setaceus germinated in a wide range of temperature, under canopy and in open areas, but in complete darkness they did not germinate.O efeito da luz e da temperatura sobre a germinação de sementes de Hylocereus setaceus foi analisado sob condições de incubações isotérmicas. Hylocereus setaceus é uma espécie ameaçada de extinção, que ocorre na Mata Atlântica e florestas estacionais semideciduais. A temperatura mínima para a germinação está entre 5 e 10°C e a máxima entre 45 e 50°C. A temperatura ótima está entre 25ºC e 30°C. A participação do fitocromo, através da resposta de fluência muito baixa, no controle da germinação de sementes foi determinada através de incubações das sementes em um gradiente de fotoequilíbrio do fitocromo. Os resultados apresentados no presente trabalho indicam que sementes de Hylocereus setaceus germinam em uma ampla faixa de temperatura, sob a sombra de vegetação e em áreas abertas, embora não germinem em condições de ausência completa de luz.

  6. USO, MANEJO Y CONSERVACIÓN DE “yosú”, Stenocereus griseus (CACTACEAE, EN LA ALTA GUAJIRA COLOMBIANA

    Directory of Open Access Journals (Sweden)

    SORAYA VILLALOBOS

    2007-01-01

    Full Text Available En este trabajo se caracteriza el sistema de uso, manejo, demanda y oferta potencial de Stenocereus griseus, un cactus columnar muy utilizado por la comunidad indígena Wayúu en la Guajira, Caribe colombiano. Actualmente el uso de S. griseus se restringe a la recolección de madera seca (“yotojoro” para construcción de viviendas, la recolección de frutos para la alimentación, de tallos jóvenes para alimentos de cabras y para utilizarlos en cercas vivas. Una evaluación de los factores que afectan las pobla- ciones permitió determinar cinco categorías de daño: las ocasionadas por corte con machete (tipo I, insectos (tipo II, cabras (tipo III, aves (tipo IV y viento (tipo V. La demanda anual de “yotojoro” está sujeta a la necesidad de construcción de los habitantes del área, que es definida como ocasional. El método actual de cosecha y extracción del mismo no está generando desequilibrio local en el mantenimiento de la especie. La demanda de cardones para cercas vivas y pastoreo es constante durante todo el año y es la que genera el mayor impacto de daño. Finalmente, se discuten los principales problemas de conservación de la especie y se recomienda establecer alter- nativas de manejo para las actividades de pastoreo.

  7. Occurrence and characterisation of calcium oxalate crystals in stems and fruits of Hylocereus costaricensis and Selenicereus megalanthus (Cactaceae: Hylocereeae).

    Science.gov (United States)

    Viñas, María; Jiménez, Víctor M

    2016-10-01

    Detailed description about occurrence of calcium oxalate (CaOx) crystals in the edible vine cactus species Hylocereus costaricensis and Selenicereus megalanthus is scarce. Therefore, we evaluated and characterized the presence, morphology and composition of CaOx crystals in both species. Crystals were isolated from greenhouse and in vitro vegetative stems, and from ripe fruit peels and pulp by enzymatic digestion and density centrifugation and quantified with a haemocytometer. Morphologies were studied using scanning electron microscopy, elemental composition with energy-dispersive X-ray spectroscopy and salt composition with X-ray powder diffraction. Analyses conducted confirmed that isolated crystals were exclusively composed by CaOx, both mono- and dihydrated. Highest crystal contents were measured in greenhouse stems, followed by the fruit peels. While very few crystals were quantified in in vitro plants, they were not detected in the fruit pulp at all, which is of advantage for its human consumption and could be linked to mechanisms of seed dispersal through animals. Different crystal morphologies were observed, sometimes varying between genotypes and tissues analysed. This is the first work known to the authors with a detailed characterization of CaOx crystals in vine cacti.

  8. Richness of endophytic fungi isolated from Opuntia ficus-indica Mill. (Cactaceae) and preliminary screening for enzyme production.

    Science.gov (United States)

    Bezerra, J D P; Santos, M G S; Svedese, V M; Lima, D M M; Fernandes, M J S; Paiva, L M; Souza-Motta, C M

    2012-05-01

    Opuntia ficus-indica Mill. (forage cactus) is farmed with relative success in the semi-arid region of the Brazilian northeast for commercial purposes, particularly as forage and food. Endophytic microorganisms are those that can be isolated inside plant tissues and can be a new source to production of enzymes with different potentialities. The objective of this study was to describe the richness of endophytic fungi from O. ficus-indica and to detect the capacity of these species to produce extracellular hydrolytic enzymes. Forty-four endophytic fungi species were isolated. Among them, the most commonly found were Cladosporium cladosporioides (20.43%) and C. sphaerospermum (15.99%). Acremonium terricola, Monodictys castaneae, Penicillium glandicola, Phoma tropica and Tetraploa aristata are being reported for the first time as endophytic fungi for Brazil. The majority of isolated fungi exhibited enzymatic potential. Aspergillus japonicus and P. glandicola presented pectinolytic activity. Xylaria sp. was the most important among the other 14 species with positive cellulase activity. All 24 isolates analysed were xylanase-positive. Protease was best produced by isolate PF103. The results indicate that there is a significant richness of endophytic fungi in O. ficus-indica, and that these isolates indicate promising potential for deployment in biotechnological processes involving production of pectinases, cellulases, xylanases and proteases.

  9. Diversidade de fungos endofíticos de mandacaru (Cereus jamacaru DC., Cactaceae) em áreas sucessionais de Caatinga

    OpenAIRE

    2013-01-01

    Fungos endofíticos vivem dentro dos tecidos sadios de plantas e podem promover tolerância das espécies hospedeiras para diferentes estresses ambientais. No entanto, a maioria dos estudos tem sido de plantas em ambientes úmidos e são poucos os relatos dos benefícios de tais associações com plantas de ambientes extremos. O objetivo deste trabalho foi analisar a diversidade de fungos endofíticos de Cereus jamacaru em três diferentes áreas de sucessão ecológica da floresta tropical seca brasileir...

  10. 7 CFR 318.13-16 - Regulated articles allowed interstate movement subject to specified conditions.

    Science.gov (United States)

    2010-01-01

    ...)(i) Puerto Rico Cactus Cactaceae Whole plant (b)(2)(iv), (b)(3)(ii) Okra Abelmoschus escuelentus.... Virgin Islands Cactus Cactaceae Whole plant (b)(2)(iv), (b)(3)(ii) Okra Abelmoschus escuelentus Fruit...

  11. Developing Dynamic Reference Models and a Decision Support Framework for Southeastern Ecosystems: An Integrated Approach

    Science.gov (United States)

    2015-06-01

    Mitchella repens Rubiaceae forb semiruderal Myrica cerifera Myricaceae woody-S,E semiruderal Opuntia humifusa Cactaceae forb semiruderal Opuntia...pusilla Cactaceae forb semiruderal Panicum verrucosum Poaceae graminoid semiruderal Panicum virgatum Poaceae graminoid semiruderal Paronychia patula

  12. Class III Survey and Testing of Cultural Resources in Proposed Flood Control System Right-of-Way, Southeastern El Paso, El Paso County, Texas

    Science.gov (United States)

    1988-05-01

    snakeweed). Flotation results suggest that Amaronthus (pigweed), Cactaceae (probably pricklypear), Cleome (beeweed), Phacelia (scorpionweed...pigweed 1 ’ Cactaceae , cf. Opuntia IN.% pricklypear 11 Cleome beeweed Phacelia scorpionweed 1 Portulaca purslane 15 3 Suaeda seepweed 16 Unknown 1

  13. Final Environmental Impact Statement MX: Buried Trench Construction and Test Project.

    Science.gov (United States)

    1977-11-01

    CACTACEAE : Carnegiea gigantea - sanuaro Ferocactus wis2izeni - barrel cactus Mamnillaria tetrancstra - pincushion cactus Opuntia acanthocarpa - buckhorn...families: lliaceae (lily family), amaryllidaceae (amaryllis family), orchidaceae (orchid family), crassulaceae (orpine family), cactaceae (cactus family

  14. Field Surveys, IOC Valleys. Volume II, Part II. Biological Resources Survey, Pine and Wah Wah Valleys, Utah.

    Science.gov (United States)

    1981-08-01

    members of the family Cactaceae : Coryphantha vivipara, Sclerocactus pubispinus, Opuntia spp., and Echinocereus engelmannii. The individuals were widely...Sclerocactus pubispinus (family Cactaceae ) was found on Sites 2/16, 3/6, 3/12, and 3/14. Coryphantha vivipara (family cactaceae ), a taxon Currently Under...family Cactaceae : Echinocereus engelmanii, Echinocereus sp., Opuntia erinacea, Opuntia sp., Sclerocactus pubispinus, Sclerocactus sp., and Coryphantha

  15. Environmental Impact Analysis Process. Environmental Assessment Proposed SMC Military Family Housing, San Pedro, California

    Science.gov (United States)

    1998-12-01

    BUXACEAE Buxus microphylla var. japonica Japanese boxwood CACTACEAE Opuntia ficus-indica Indian-fig CAMPANULACEAE Campanula spp. bellflowers...sativus black mustard wild radish CACTACEAE Opuntia littoralisa coast prickly pear CHENOPODIACEAE Salsola tragus Russian thistle, tumbleweed...BUXACEAE Buxus microphylla var. japonica Japanese boxwood CACTACEAE Opuntia ficus indica Indian fig CAPRIFOLIACEAE Lonicera hildebrandiana Lonicera

  16. Small Sites in the Central Hueco Bolson: A Final Report on Project 90-11

    Science.gov (United States)

    1998-01-01

    members of the families Malvaceae, Cactaceae , or Nyctaginaceae. Pollen concentration values were computed for each sample using the following formula: K...four o’clock family Atriplex Saltbush Pinus Pine Cactaceae Cactus family Poaceae Grass family Caryophyllaceae Pink family Populus Cottonwood...Results. Catalog Number CLES Arte- misia Platy- opuntia Cactaceae Ephedra Zea mays Indeter- minate Marker Trans Sum % Indeter. Con. Raw

  17. Características físicas, químicas e organolépticas de quipá (Tacinga inamoena, Cactaceae)

    OpenAIRE

    SOUZA, Andréa Carla Mendonça de; Gamarra-Rojas,Guillermo; Andrade,Samara Alvachian Cardoso; Guerra,Nonete Barbosa

    2007-01-01

    A Opuntia ficus-indica tem-se destacado como principal produtora de frutos e forragens, motivo pelo qual tem sido bastante pesquisada. Além desta espécie, no semi-árido do Nordeste é encontrada a Tacinga inamoena, planta nativa, cujo fruto, embora também utilizado pelo agricultor como alternativa alimentar, não foi objeto de nenhuma pesquisa até o momento, justificando este trabalho para avaliar seu potencial nutricional e industrial, por meio das características organolépticas, químicas e fí...

  18. The molecular phylogeny of Rebutia (Cactaceae) and its allies demonstrates the influence of paleogeography on the evolution of South American mountain cacti.

    Science.gov (United States)

    Ritz, Christiane M; Martins, Ludwig; Mecklenburg, Rainer; Goremykin, Vadim; Hellwig, Frank H

    2007-08-01

    The tropical Andes harbor a major part of the world's plant biodiversity. The montane cacti of the tribes Browningieae, Cereeae, and Trichocereeae underwent extensive radiation and thus are well suited as a model group to study the diversification of Andean plants. We reconstructed their phylogeny employing three noncoding chloroplast regions and explained it in the context of the geological history of South America. We found that the clade of cephalia-bearing cacti with naked pericarpels is centered in northeastern Brazil, whereas almost all other clades comprise Andean species. The spatial split between the clades was probably caused by the Andean uplift and the concurrent formation of intracontinental marine basins in the Tertiary. The phylogenetic reconstructions based on parsimony and Bayesian approaches do not reflect the traditional delimitation of the tribes and of the large genera. Our results suggest that Rebutia s.l. and Echinopsis s.l. are not monophyletic and that Sulcorebutia, Weingartia, and Cintia should be united into one genus. Even though this "Weingartia-complex" and the genus Gymnocalycium are similar in size and morphological diversity, Gymnocalycium has a very high molecular divergence suggesting a comparably older radiation.

  19. Research on tissue culture of four kinds of cactaceae plant%4种仙人掌科植物的组织培养研究

    Institute of Scientific and Technical Information of China (English)

    关丽霞; 韩德伟; 谢永刚; 刘冰; 杨倩

    2009-01-01

    以仙人掌科4种植物的嫩茎或子球为外植体.研究了不同培养基对其诱导和增殖的影响,同时还进行了生根和移栽试验.结果表明:珊瑚树、黄毛掌和山影掌嫩茎的最佳诱导培养基为MS+BA2.0 mg/L+NAA0.2 mg/L、最适增殖培养基为MS+BA0.5 mg/L+NAA0.2 mg/L,豪氏沙布疣球子球的最佳诱导培养基为MS+BA3.0 mg/L+NAA0.2 m/L、最适增殖培养基为MS+BA1.0 mg/L+NAA0.2 mg/L;4种仙人掌科植物在1/2 MS+IBA0.5 mg/L培养基上均能良好生根,将生根苗移栽到蛭石:河沙为1:1的混合基质中成活率达80%以上.

  20. Propagation Technologies of Edible Cactus Opuntia milpa alta Haw(Cactaceae)%米邦塔食用仙人掌种苗繁殖技术研究

    Institute of Scientific and Technical Information of China (English)

    向青云; 蔡永强; 王祖泽; 陈家龙; 毛堂芬

    2004-01-01

    采用组织培养、切块、嫁接和扦插4种繁殖方法,繁育米邦塔食用仙人掌种苗.结果表明,组织培养、切块繁殖两种方法较其它方法繁殖系数高,但后代植株弱小,培育成标准种苗所需周期较长;嫁接繁殖抽生掌片数量及质量不佳;扦插繁殖简单易行,生产周期短,相对成本低,建议在生产上推广运用.

  1. Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome.

    Science.gov (United States)

    Monteiro, E R; Strioto, D K; Meirelles, A C S; Mangolin, C A; Machado, M F P S

    2015-12-15

    Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged from 95.3% for the primer combination E-AAG/M-CTT to 100% for E-ACC/M-CAT, E-ACC/M-CAA, E-AGC/M-CAG, E-ACT/M-CTA, and E-AGG/M-CTG. The largest number of informative markers (126) was detected using the primer combination E-AAC/M-CTA. Polymorphism of the amplified DNA fragments ranged from 72.55% (in sample from Piauí State) to 82.79% (in samples from Rio Grande Norte State), with an average of 75.39%. Despite the high genetic diversity of AFLP markers in xiquexique, analysis using the STRUCTURE software identified relatively homogeneous clusters of xiquexique from the same location, indicating a differentiation at the molecular level, among the plant samples from different regions of the Caatinga biome. The AFLP methodology identified genetically homogeneous and contrasting plants, as well as plants from different regions with common DNA markers. Seeds from such plants can be used for further propagation of plants for establishment of biodiversity conservation units and restoration of degraded areas of the Caatinga biome.

  2. Dispersal of Dactylopius opuntiae Cockerell (Homoptera: Dactylopiidae, a biological control agent of Opuntia stricta (Haworth. Haworth. (Cactaceae in the Kruger National Park

    Directory of Open Access Journals (Sweden)

    L.C. Foxcroft

    2000-07-01

    Full Text Available Chemical control efforts, the introduction of Cactoblastis cactorum and attempted releases of Dactylopius opuntiae Cockerell into the expanding infestation of Opuntia stricta in the Skukuza region of the Kruger National Park (KNP have had limited suc- cess in preventing the spread and densification of 0. stricta. To boost the biological control component, a new strain of D. opuntiae was introduced into KNP during 1997. The new strain established readily and has destroyed large clumps of plants in the vicin- ity of the release site. A large-scale redistribution programme with D. opuntiae is now needed to exploit this biological control agent to the full. In order to match the frequency of manual releases with the natural rates of spread of the insects, surveys were conducted under field conditions to determine the dispersal abilities ofD. opuntiae, with regard to rate and direction of movement. Dispersal of D. opuntiae was found to be slow and restricted and that the insects need to be redistributed by placing them onto plants at approximately 10 m intervals to ensure that they become quickly and evenly distributed on the weed. This information will be crucial in the revision of the integrated management plan for 0. stricta in the KNP, in integrating the cochineal and other control mechanisms.

  3. Culture in Vitro of Edible Cactus Opuntia milpa alta Haw (Cactaceae)%米邦塔食用仙人掌试管快速繁殖试验

    Institute of Scientific and Technical Information of China (English)

    刘晓燕; 向青云; 蔡永强; 刘玲玲; 王祖泽; 陈家龙

    2004-01-01

    以米邦塔食用仙人掌幼龄茎为外植体,研究从诱导培养,增殖继代,生根培养,到瓶外移植各阶段试管快繁的若干影响因子.试验结果表明,在附加3.0mg/LBA+0.2 mg/L NAA的MS培养基上诱导效果较好.40d后转入附加2.0mg/L BA+0.2mg/L NAA的MS培养基上继代培养,以30d为一个增殖继代周期,增殖倍率达6.2.随继代次数增加,可减少BA用量;生根培养阶段以1/2 MS+0.1mg/LNAA的培养基生根效果最好.无机盐浓度减半,糖用量减少1/3,有利于生根与壮苗;移栽时注意保持水分平衡,选择栽植介质,控制杂菌污染以及选择适宜的光照和温度条件.

  4. 仙人掌科花粉形态的扫描电镜研究%STUDIES ON POLLEN MORPHOLOGY IN CACTACEAE USING SCANNING ELECTRON MICROSCOPE

    Institute of Scientific and Technical Information of China (English)

    杨德奎

    2010-01-01

    利用扫描电子显微镜,对仙人掌科7属8种植物的花粉形态进行观察研究.结果显示:蟹爪兰属、仙人掌属的花粉粒为球形,具散孔;昙花属的花粉粒为球形,具3沟;长疣球属的花粉粒为球形,具4沟;子孙球属的花粉粒为近球形,具3沟;令箭荷花属花粉粒为长球形,具3孔沟;乳突球属花粉粒为长球形,具3沟.花粉粒大小、形状和外壁雕纹属、种间差异显著.

  5. Extensive 5.8S nrDNA polymorphism in Mammillaria (Cactaceae) with special reference to the identification of pseudogenic internal transcribed spacer regions.

    Science.gov (United States)

    Harpke, Doerte; Peterson, Angela

    2008-05-01

    The internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, ITS2) represents the most widely applied nuclear marker in eukaryotic phylogenetics. Although this region has been assumed to evolve in concert, the number of investigations revealing high degrees of intra-individual polymorphism connected with the presence of pseudogenes has risen. The 5.8S rDNA is the most important diagnostic marker for functionality of the ITS region. In Mammillaria, intra-individual 5.8S rDNA polymorphisms of up to 36% and up to nine different types have been found. Twenty-eight of 30 cloned genomic Mammillaria sequences were identified as putative pseudogenes. For the identification of pseudogenic ITS regions, in addition to formal tests based on substitution rates, we attempted to focus on functional features of the 5.8S rDNA (5.8S motif, secondary structure). The importance of functional data for the identification of pseudogenes is outlined and discussed. The identification of pseudogenes is essential, because they may cause erroneous phylogenies and taxonomic problems.

  6. Predation of Opuntia monacantha (Willd. Haw. (Cactaceae by Cactoblastis cactorum (Lepidoptera: Pyralidae in a sand bank area of Santa Catarina island, south Brazil

    Directory of Open Access Journals (Sweden)

    Afonso Inácio Orth

    2006-09-01

    Full Text Available The genus Opuntia is worldwide known for its ecological, ornamental and agronomic importance. Some species became pests in the countries in which they where introduced, and as biological control, Cactoblastis cactorum (Lepidoptera: Pyralidae larvae, originary from Argentina, were used. However, the effect of the attack of this piralid on native cactus has yet not been elucidated. The objective of this study was to detect and to quantify the predation of C. cactorum on Opuntia monacantha. The study was carried out from September to November of 2004, along pre-defined tracks, on a sand bank vegetation area, between the Mole and Galheta beaches in the Santa Catarina island (27º35’83.1’’S e 48º25’70.6’’W. All the studied plants (n = 20 presented some damage caused by C. cactorum. The proportion of unpredated cladodes (68% and fruits (85% was higher than the predated ones. Terminal cladodes were highly predated structures and presented the highest number of larvae inside. Seed loss in the predated fruits was high. The remaining areole of the predated cladodes and fruits differentiated into sprouts and routs and formed new plants. O. monacantha, despite of being predated by C. cactorum larvae, apparently possess defense mechanisms which assure the maintenance of its populations.

  7. The effect of temperature on the germination of Melocactus violaceus Pfeiff. (Cactaceae), a threatened species in restinga sandy coastal plain of Brazil.

    Science.gov (United States)

    Zamith, Luiz R; Cruz, Denise D; Richers, Bárbara T T

    2013-01-01

    Melocactus violaceus is an endangered species due to habitat destruction and the overcollection of this species for ornamental use. The aim of this study was to test the effect of different temperatures on the germination of M. violaceus. Three treatments were conducted: a constant temperature of 25ºC, a 20-35ºC alternating temperature, both inside germination chamber, and an alternating temperature under room temperature (mean temperature ranged from 25-37ºC). The final seed germination rates at the alternating temperature treatments were not significantly different (65% in the seed germinator and 62.5% at room condition). However, both treatments with alternating temperatures had significantly higher germination rates compared to the treatment kept at the constant temperature (8%). Our study showed that alternating temperatures between 20 and 37ºC provides satisfactory conditions to induce a high percentage of seed germination of M. violaceus, without the passage of seeds through the digestive tract of its natural disperser, the lizard Tropidurus torquatus. This condition contributes to efficiently producing seedlings that can be reintroduced into conservation areas or used as ornamentals that may help reduce the overcollection of the remaining native populations.

  8. Germinación de tres especies de Cephalocereus (Cactaceae endémicas delIstmo de Tehuantepec, México

    Directory of Open Access Journals (Sweden)

    Maria Luisa Bárcenas-Argüello

    2015-08-01

    Full Text Available Se estudió el proceso germinativo de tres especies deCephalocereus (C. apicicephalium, C. nizandensis, C. totolapensisendémicas del Istmo de Tehuantepec, Oaxaca, México, bajo las hipótesis de que las semillas perderán la capacidad germinativa a medida que pasen los días después de la recolección y que la germinación es diferente entre especies, pero no entre poblaciones de la misma especie. Para cada una de las especies estudiadas, se recolectaron semillas de poblaciones diferentes y se sembraron en cuatro fechas distintas, colocando 30 semillas en dos o tres cajas de Petri. Las cajas se colocaron en una cámara de ambiente controlado y la germinación se registró diariamente durante 30 días, considerando una semilla germinada cuando emergía la radícula. Los porcentajes de germinación (38.8% enC. totolapensis, 55.1% en C.apicicephaliumy la velocidad de germinación (1.6 enC. apicicephalium, Guiengola-2 a 24 enC. apicicephalium, Guiengola-1 yC. nizandensis, La Mata fueron significativamente diferentes entre especies y entre poblaciones (P

  9. Cross-species amplification of microsatellites reveals incongruence in the molecular variation and taxonomic limits of the Pilosocereus aurisetus group (Cactaceae).

    Science.gov (United States)

    Moraes, Evandro M; Perez, Manolo F; Téo, Mariana F; Zappi, Daniela C; Taylor, Nigel P; Machado, Marlon C

    2012-09-01

    The Pilosocereus aurisetus group contains eight cactus species restricted to xeric habitats in eastern and central Brazil that have an archipelago-like distribution. In this study, 5-11 microsatellite markers previously designed for Pilosocereus machrisii were evaluated for cross-amplification and polymorphisms in ten populations from six species of the P. aurisetus group. The genotypic information was subsequently used to investigate the genetic relationships between the individuals, populations, and species analyzed. Only the Pmac101 locus failed to amplify in all of the six analyzed species, resulting in an 88 % success rate. The number of alleles per polymorphic locus ranged from 2 to 12, and the most successfully amplified loci showed at least one population with a larger number of alleles than were reported in the source species. The population relationships revealed clear genetic clustering in a neighbor-joining tree that was partially incongruent with the taxonomic limits between the P. aurisetus and P. machrisii species, a fact which parallels the problematic taxonomy of the P. aurisetus group. A Bayesian clustering analysis of the individual genotypes confirmed the observed taxonomic incongruence. These microsatellite markers provide a valuable resource for facilitating large-scale genetic studies on population structures, systematics and evolutionary history in this group.

  10. Risk analysis of pests on imported Cactaceae seedlings%进境仙人掌种苗有害生物风险分析

    Institute of Scientific and Technical Information of China (English)

    方志鹏; 廖富荣; 林玲玲; 黄蓬英; 陈红运

    2015-01-01

    本文根据FAO/IPPC秘书处制订的有关植物检疫措施国际标准和《中华人民共和国国家标准——进出境植物和植物产品有害生物风险分析技术要求》(GB/T 20879-2007),对我国进境仙人掌种苗可能携带的有害生物进行风险分析,可能携带的有害生物种类132种(属),确定潜在检疫性有害生物75种,对75种潜在检疫性有害生物进一步评估,确定中风险以上的有害生物20种,建议将这20种有害生物作为进境仙人掌种苗的检疫性有害生物写入议定书中,并提出降低检疫性有害生物传入的风险管理措施.

  11. Diversity and antifungal activity of the endophytic fungi associated with the native medicinal cactus Opuntia humifusa (Cactaceae) from the United States.

    Science.gov (United States)

    Silva-Hughes, Alice F; Wedge, David E; Cantrell, Charles L; Carvalho, Camila R; Pan, Zhiqiang; Moraes, Rita M; Madoxx, Victor L; Rosa, Luiz H

    2015-06-01

    The endophytic fungal community associated with the native cactus Opuntia humifusa in the United States was investigated and its potential for providing antifungal compounds. A hundred-eight endophytic fungal isolates were obtained and identified by molecular methods into 17 different taxa of the genera Alternaria, Aureobasidium, Biscogniauxia, Cladosporium, Cryptococcus, Curvularia, Diaporthe, Epicoccum, Paraconiothyrium, Pestalotiopsis and Phoma. The most frequent species associated with O. humifusa were Alternaria sp. 3, Aureobasidium pullulans and Diaporthe sp. The fungal community of O. humifusa had a high richness and diversity; additionally, the species richness obtained indicates that the sample effort was enough to recover the diversity pattern obtained. Six extracts of endophytes showed antifungal properties and (1)H NMR analyses of the extracts of Alternaria sp. 5 Ohu 8B2, Alternaria sp. 3 Ohu 30A, Cladosporium funiculosum Ohu 17C1 and Paraconiothyrium sp. Ohu 17A indicated the presence of functional groups associated with unsaturated fatty-acid olefinic protons and fatty acid methylene and methyl protons. GC-FID analysis of these extracts confirmed the presence of a mixture of different fatty acids. The (1)H NMR analyses of Biscogniauxia mediterranea Ohu 19B extracts showed the presence of aromatic compounds. From the extract of B. mediterranea we isolated the compound 5-methylmellein that displayed moderate antifungal activity against the phytopathogenic fungi Phomopsis obscurans. Our results suggest that native medicinal cacti of the United States can live symbiotically with rich and diverse endophytic communities and may be a source of bioactive molecules, including those able to inhibit or control plant disease pathogens.

  12. The effect of temperature on the germination of Melocactus violaceus Pfeiff. (Cactaceae, a threatened species in restinga sandy coastal plain of Brazil

    Directory of Open Access Journals (Sweden)

    LUIZ R. ZAMITH

    2013-06-01

    Full Text Available Melocactus violaceus is an endangered species due to habitat destruction and the overcollection of this species for ornamental use. The aim of this study was to test the effect of different temperatures on the germination of M. violaceus. Three treatments were conducted: a constant temperature of 25ºC, a 20-35ºC alternating temperature, both inside germination chamber, and an alternating temperature under room temperature (mean temperature ranged from 25-37ºC. The final seed germination rates at the alternating temperature treatments were not significantly different (65% in the seed germinator and 62.5% at room condition. However, both treatments with alternating temperatures had significantly higher germination rates compared to the treatment kept at the constant temperature (8%. Our study showed that alternating temperatures between 20 and 37ºC provides satisfactory conditions to induce a high percentage of seed germination of M. violaceus, without the passage of seeds through the digestive tract of its natural disperser, the lizard Tropidurus torquatus. This condition contributes to efficiently producing seedlings that can be reintroduced into conservation areas or used as ornamentals that may help reduce the overcollection of the remaining native populations.

  13. The Adaptability of Edible Cactus Opuntia milpa alta Haw(Cactaceae)%米邦塔食用仙人掌适应性研究

    Institute of Scientific and Technical Information of China (English)

    向青云; 蔡永强; 陈家龙; 王祖泽; 刘晓燕; 邵宇

    2004-01-01

    采用不同栽培方式在贵州南部和中部进行米邦塔食用仙人掌适应性栽培试验.结果表明,供试品种均表现出较强的环境适应性和抗逆性,年平均产量为2 358.7~3153.9kg/667m2;菜片中Vc、矿质营养元素和氨基酸含量较高,其中钙元素含量比高钙蔬菜高43.9%.适宜在贵州南部低热河谷地区露地栽培和中部地区设施栽培.

  14. Minireview on the researches of natural active components in Cactaceae%仙人掌的天然活性成分研究回顾

    Institute of Scientific and Technical Information of China (English)

    林琳; 鲍世铨; 王钊

    2002-01-01

    该综述参阅了近20年来国内外有关仙人掌的研究报导和有关书刊,对各类仙人掌中的天然活性成分,如生物碱类尤其是墨斯卡林、黄酮类如黄酮醇、甾醇类如谷甾醇以及其他成分如油脂、蛋白质、多糖类、微量元素等的研究状况进行了全面地综合和归类,对仙人掌的开发应用情况及前景做了细致的分析和展望.

  15. Landscape management and domestication of Stenocereus pruinosus (Cactaceae in the Tehuacán Valley: human guided selection and gene flow

    Directory of Open Access Journals (Sweden)

    Parra Fabiola

    2012-08-01

    Full Text Available Abstract Background Use of plant resources and ecosystems practiced by indigenous peoples of Mesoamerica commonly involves domestication of plant populations and landscapes. Our study analyzed interactions of coexisting wild and managed populations of the pitaya Stenocereus pruinosus, a columnar cactus used for its edible fruit occurring in natural forests, silviculturally managed in milpa agroforestry systems, and agriculturally managed in homegardens of the Tehuacán Valley, Mexico. We aimed at analyzing criteria of artificial selection and their consequences on phenotypic diversity and differentiation, as well as documenting management of propagules at landscape level and their possible contribution to gene flow among populations. Methods Semi-structured interviews were conducted to 83 households of the region to document perception of variation, criteria of artificial selection, and patterns of moving propagules among wild and managed populations. Morphological variation of trees from nine wild, silviculturally and agriculturally managed populations was analyzed for 37 characters through univariate and multivariate statistical methods. In addition, indexes of morphological diversity (MD per population and phenotypic differentiation (PD among populations were calculated using character states and frequencies. Results People recognized 15 pitaya varieties based on their pulp color, fruit size, form, flavor, and thorniness. On average, in wild populations we recorded one variety per population, in silviculturally managed populations 1.58 ± 0.77 varieties per parcel, and in agriculturally managed populations 2.19 ± 1.12 varieties per homegarden. Farmers select in favor of sweet flavor (71% of households interviewed and pulp color (46% mainly red, orange and yellow. Artificial selection is practiced in homegardens and 65% of people interviewed also do it in agroforestry systems. People obtain fruit and branches from different population types and move propagules from one another. Multivariate analyses showed morphological differentiation of wild and agriculturally managed populations, mainly due to differences in reproductive characters; however, the phenotypic differentiation indexes were relatively low among all populations studied. Morphological diversity of S. pruinosus (average MD = 0.600 is higher than in other columnar cacti species previously analyzed. Conclusions Artificial selection in favor of high quality fruit promotes morphological variation and divergence because of the continual replacement of plant material propagated and introduction of propagules from other villages and regions. This process is counteracted by high gene flow influenced by natural factors (pollinators and seed dispersers but also by human management (movement of propagules among populations, all of which determines relatively low phenotypic differentiation among populations. Conservation of genetic resources of S. pruinosus should be based on the traditional forms of germplasm management by local people.

  16. Anatomía y Morfología de las Especies Silvestres del Género Hylocereus (Berger) Britton & Rose (Cactaceae) en México

    OpenAIRE

    García Aguilar, María de los Ángeles

    2012-01-01

    Se estudió la anatomía caulinar y la morfología de diez poblaciones del género Hylocereus con distribución en México con el objetivo de evaluar su variación y su contribución en la sistemática del género. En cada una de las localidades muestreadas se colectaron de 3 a 5 individuos fenológicamente maduros a fin de garantizar la mayor homogeneidad en el material. Para la obtención de los caracteres anatómicos se tomaron muestras de ramas adultas en la parte apical, media y b...

  17. Effectiveness of bats as pollinators of Stenocereus stellatus (Cactaceae) in wild, managed in situ, and cultivated populations in La Mixteca Baja, central Mexico.

    Science.gov (United States)

    Arias-Cóyotl, Ethel; Stoner, Kathryn E; Casas, Alejandro

    2006-11-01

    Stenocereus stellatus is an endemic, self-incompatible, columnar cactus found in central Mexico where many of its wild populations have been fragmented. As an economically important species of fruit-producing cactus, S. stellatus occurs in wild, managed in situ, and cultivated populations. The objectives of this study were to determine the effective pollinators of S. stellatus, to compare pollinator visits and reproductive parameters among the three types of populations, and to determine if nectar feeding-bats are moving among populations. Effective pollinators were the nectarivorous bats Choeronycteris mexicana, Leptonycteris curasoae, and L. nivalis. Fewer total visits per flower per night and fewer visits by Choeronycteris were observed in cultivated populations, while the opposite pattern was observed for Leptonycteris. One aggressive interaction was filmed in which Choeronycteris was physically displaced by Leptonycteris, and Choeronycteris visits were significantly affected by Leptonycteris visits. Cultivated populations received more pollen grains and had more fruit set. Variation in pollinator visits between different populations and the consequent effects on reproductive success were likely a result of competition between bat species, and differences in foraging and in sensitivity of bat species to human populations. Three marked L. curasoae traveled 15 km from their roosting site to their foraging area, and one visited cultivated and managed populations, suggesting that this species may be particularly important in moving pollen among populations.

  18. Analysis of the factors that affect the distribution and abundance of three Neobuxbaumia species (Cactaceae) that differ in their degree of rarity

    Science.gov (United States)

    Ruedas, Marcela; Valverde, Teresa; Zavala-Hurtado, José Alejandro

    2006-03-01

    We studied three species of columnar cacti in the genus Neobuxbaumia which differ in their degree of rarity: Neobuxbaumia macrocephala (the rarest), Neobuxbaumia tetetzo (intermediate), and Neobuxbaumia mezcalaensis (the most common). To investigate the ecological factors that limit their distribution and abundance, we surveyed 80 localities within the region of Tehuacan-Cuicatlán, in Central Mexico. At each locality we measured several environmental variables, and the density of the Neobuxbaumia populations present. We used a principal component analysis (PCA) to identify the factors that are associated to the presence/absence of each species. Additionally, we carried out multiple regressions between environmental variables and population density to test whether the variation in these variables was related to changes in abundance. The results show that factors significantly affecting the distribution of these species are mean annual temperature, altitude, rainfall, and soil properties such as texture and organic matter content. N. mezcalaensis reaches maximum population densities of 14,740 plants per ha (average density = 3943 plants per ha) and is associated with localities with relatively abundant rainfall. N. tetetzo shows maximum population densities of 14,060 plants per ha (average = 3070 plants per ha), and is associated with sites located at high latitudes and with high phosphorous content in the soil. The rarest species, N. macrocephala, shows maximum densities of 1180 plants per ha (average = 607 plants per ha) and is associated with localities with high soil calcium content. The distribution of this species is limited to sites with specific values of the environmental variables recorded, conferring it a high habitat specificity which accounts for its rarity.

  19. Myxomycetes associated with Cactaceae in sub-humid (agreste and semi-arid (sertão regions of Pernambuco, Brazil

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    Inaldo do Nascimento Ferreira

    2011-06-01

    Full Text Available Studies that indicate the presence of Myxomycetes in arid and semi-arid regions of the planet have revealed new taxons and high species diversity. In those environments, the Myxomycetes occupy special microhabitats like those offered by succulent plants. Aiming to expand the knowledge of species that occur in semi-arid environments we investigated the occurrence of Myxomycetes on Cereus jamacaru (mandacaru, Melocactus bahiensis (coroa de frade, Opuntia ficus-indica (palma forrageira and Pilocereus gounellei (xique-xique – cacti that compose the natural landscape of the sub-humid (agreste and semi-arid (sertão regions of Pernambuco, Brazil. We obtained 158 specimens of Myxomycetes (106 field collections; 52 in moist chamber cultures, representing 16 species distributed in nine genera, with high taxonomic diversity (S/G= 0.63-1.5. All of the identified species are new records for the municipalities studied. Comatricha pulchella, Didymium nigripes and Physarum bogoriense are mentioned for the first time in Caatinga areas in the state of Pernambuco, and Didymium squamulosum and Physarum echinosporum are new records for this biome. Physarum compressum and Badhamia melanospora were the most abundant and frequent species, characterized as constant in the studied myxobiota.

  20. MX Siting Investigation. DTN/OBTS Field Surveys. Volume III. Biological Resources Nevada and Utah.

    Science.gov (United States)

    1981-11-30

    Tumble mustard F MYCW Lepidium fremontii Desert pepperweed F CW,W CACTACEAE Echinocereus engelmannii Hedgehog cactus S MY Ferocactus acant’,jodes...Lepidium sp. Pepperweed F MS Sisymbrium altissimum Tumble mustard F Js Sisymbrium sp. Tumble mustard F BS CACTACEAE Echinocereus engelmannii Engelmannii...Streptanthella F BS,W lorostris StetnhlaTwist flower F PJ cordatus CACTACEAE Echinocereus Hedgehog cactus S BIS triglochidiatus Opuntia erinacea Prickly pear S PJ

  1. Distribution and Status of Sclerocactus polyancistrus on the Naval Weapons Center-A Survey.

    Science.gov (United States)

    1982-10-01

    Doma ntered) NWC TP 6403 PREFACE Sclerocactus polyancistrus, an attractive member of the Cactaceae family, has been reported to be decreasing in...N.L. & J.N. Rose. 1922. Cactaceae 3: 213-215. SYNONYMS: Echinocactus polyancistrus (Engelmann & Bigelow) Engelmann, G. 1856. Proc. Amer. Acad. 3: 272...COMMON NAMES: Mojave Fishhook Cactus Hermit Cactus Pineapple Cactus Devil’s Claw Mojave Bisnaga FAMILY: Cactaceae (Cactus) DESCRIPTION: (Non-technical

  2. Environmental Assessment Report: Geotechnical Field Investigations.

    Science.gov (United States)

    1975-06-30

    ZDNA ENDANGERED i3RA5$ICACEAE STREYANTHJS LEMMONIJ AR)IQA ENANGERED CACTACEAE LCHINOCACIUS hNO ZONrH4ALQNIU5 VAN. %10𔃺lj1l AR ZONA ENANGJERED CACTACEAC...ECHN~OCEREU!b TRIGLOCPIOIATUS 4A . APl2ONICUS ARIZONA ENDANGERED CACTAtEAE OPUNTIA 8ASILARI5 VAR. IRLEAbEl jARIZONA EN~DANGERED CACTACEAE PE0IO(ACTUS...ORADYI AliI IONA, ENDANGERED CACTAC:EAE PEDIOCACTUS PEE LE5ANUJS VAR* PEEDLESLANU5 4EtOANNERED CACTACEAE PEDIOCACTUb 540.ER a 4)%&J:AN(.cRof

  3. Morfo-anatomia do fruto (hipanto, pericarpo e semente em desenvolvimento de Pereskia aculeata Miller (Cactaceae - DOI: 10.4025/actascibiolsci.v25i2.2046 Morphology and anatomy of the fruit (hypanthium, pericarp and seed development of Pereskia aculeate Miller (Cactaceae

    Directory of Open Access Journals (Sweden)

    Sonia Maciel da Rosa

    2003-04-01

    Full Text Available O desenvolvimento do fruto (hipanto, pericarpo e semente de Pereskia aculeata Miller é analisado desde a flor em antese até a maturidade. A flor é perígina e apresenta no hipanto bractéolas verdes e acúleos. O fruto é pomáceo, tipo cactídio e caracteriza-se por apresentar hipanto carnoso, pericarpo e massa gelatinosa, formada pela destruição parcial do pericarpo e columela, onde estão imersas as sementes. A semente é exotestal e origina-se do óvulo anfítropo, bitegumentado e crassinucelado. A semente apresenta reserva perispérmica e resíduos de endosperma. O embrião é curvo e tem eixo hipocótilo-radicular longo, dois cotilédones com mesofilo homogêneo e plúmula indiferenciadaThe development of Pereskia aculeate Miller fruit (hypanthium, pericarp and seed was analyzed. The flower is perigynous and presents a hypanthium with bracteoles and aculeus. The fruit is pomaceous, type cactídio, with succulent hypanthium, pericarp, and seeds immersed in a gelatinous mass. This gelatinous mass originates from the pericarp and the columella. The seed is exotestal and develops from an amphitropous, bitegmic and crassinucelate ovule. It has perisperm and an endosperm residue. The embryo is curved and is composed of elongated hypocotyl-radicle axis, two cotyledons with uniform mesophyll, and undifferentiated plumule

  4. Morphology and anatomy of Rhipsalis cereuscula, Rhipsalis floccosa subsp. hohenauensis and Lepismium cruciforme (Cactaceae seedlings Morfología y anatomía de las plántulas de Rhipsalis cereuscula, Rhipsalis floccosa subsp. hohenauensis y Lepismium cruciforme (Cactaceae

    Directory of Open Access Journals (Sweden)

    Alan C. Secorun

    2011-03-01

    Full Text Available Rhipsalis cereuscula Haw., Rhipsalis floccosa subsp. hohenauensis (F. Ritter Barthlott et N. P. Taylor and Lepismium cruciforme (Vellozo Miquel are obligatory epiphytes that occur frequently on tree trunks of remnant forests in Maringa, Paraná state, Brazil. Morphological and anatomical analyses regarding the seedlings were carried out. The seedlings were prepared according to techniques of resin inclusions and histochemical tests. Seedlings were phanerocotyledonar and originated from seeds with operculum. The root was diarch and the hypocotyl presented transition root-stem structure. The cotyledons were sessile, reduced, with homogeneous mesophyll. The epicotyl (phylloclade presented a lot of parenchyma and reduced vascular cylinder. The 3 studied species showed anatomical characteristics similar to those described for species of Lepismium and Rhipsalis as well as other cacti.Rhipsalis cereuscula Haw., Rhipsalis floccosa subsp. hohenauensis (F. Ritter Barthlott et N. P. Taylor y Lepismium cruciforme (Vellozo Miquel son epífitos obligatorios que frecuentemente habitan en los troncos del árbol de matorrales secundarios de Maringá, Paraná, Brasil. Se analizaron la morfología y anatomía de las plántulas de estas especies. Las plántulas fueron procesadas según las técnicas de inclusión en resina y pruebas histoquímicas. Las plántulas se clasificaron como fanerocotiledonares y se originaron de semillas con opérculo. La raíz era diarca y el hipocótilo presentó estructura de transición raíz-tallo. Los cotiledones fueron sésiles, reducidos, con el mesófilo homogéneo. El epicótilo (filocladio presentó mucho parénquima y el cilindro vascular reducido. Las 3 especies presentaron características anatómicas similares a las descritas para especies de Lepismium y Rhipsalis, así como otras cactáceas.

  5. In situ Morphological Characterization of Hylocereus spp. (Fam.: Cactaceae Genotypes from Antioquia and Córdoba (Colombia / Caracterización Morfológica in situ de Genotipos de Hylocereus spp. (Fam.: Cactaceae de Antioquia y Córdoba (Colombia

    Directory of Open Access Journals (Sweden)

    Mejía Henry Andrés

    2013-08-01

    Full Text Available The genus Hylocereus includes many importantspecies for human and animal nutrition, including those known as dragon fruits. Although Colombia produces yellow pitaya on a commercial scale, there have been no advances for the red pitahaya crop. This study aimed to do an in situ evaluation of genetic material of wild red pitahaya (Hylocereus spp. from three populations located in Antioquia and Córdoba; with special interest for the fruits, taking into account the desired characteristics by the market. To achieve this, 23 descriptors of stems and fruits, measurable in the field, were chosen; and 35 individuals where located, using GPS, and described. The populations were compared using mean comparison and the similarity was evaluated with cluster analysis. The stem descriptors differed in spine number, contour and margin hardness, areole distance and stem color; the first three are important for species distinction.According to the fruit descriptors, populations 1 and 2 presented similar fruits that were different from those of population 3, for almost all variables except total soluble solids content. The fruits of population 1 and 2 only differed for pulp weight. Individual 3 of population 1 presented fruits with the best characteristics for the market / El género Hylocereus presenta varias especies importantes en la alimentación humana y animal, incluyendo aquellas conocidas como pitayas rojas. Aunque Colombia produce comercialmente pitaya amarilla, no ha avanzado en el cultivo de pitaya roja. Este trabajo se propuso caracterizar in situ materiales genéticos silvestres de pitaya roja (Hylocereus spp. provenientes de tres poblaciones de Antioquia y Córdoba, especialmente de sus frutos, según las características deseables para el mercado. Para ello,se eligieron 23 descriptores de tallos y frutos, medibles en campo; se ubicaron y georeferenciaron 35 individuos y se describieron. Las poblaciones fueron comparadas en cada uno de los descriptores usando una comparación de medias, además se evaluó la similitud entre poblaciones con un análisis de agrupamiento. Según los descriptores de tallos, hubo diferencias en: número de espinas, contorno y dureza de las márgenes, distancia entre areolas, altura yespesor de la arista, longitud y color del cladodio; las tres primeras características son importantes para diferenciar especies. Según los descriptores de fruto, las poblaciones 1 y 2 presentaron frutos más similares entre sí y diferentes de los de la población 3, en casi todas las variables excepto en el contenido de sólidos solubles totales. Los frutos de la población 1 y 2 sólo difirieron en el peso de la pulpa. El individuo 3 de la población 1 presentó frutos con mejorescaracterísticas para el mercado.

  6. Archaeological Investigations of Three Sites within the Wipp Core Area, Eddy County, New Mexico.

    Science.gov (United States)

    1985-06-01

    leaves are eaten as food (Castetter 1935:33). Cactaceae c macroMeris Texas devilshead Echinocereaus pi s Caespitose hedgehog E. fendleri Fendler hedgehog...Ountia ad.isii Davis cholla . kleiniae Klein cholla 0. ept is Christmas cactus 0. phaeacantha Prickly pear Fruits of most cactaceae are edible. The

  7. MX Siting Investigation. Preliminary Biological and Cultural Resources Inventory and Environmental Evaluation of the Proposed Operational Base Sites in Coyote Spring Valley and the Milford-Beryl Area.

    Science.gov (United States)

    1981-03-20

    Stanleya pinriata (desert prince- plume) Stanleya sp. X CACTACEAE Echinocereus engelmannii (Engel- X X X X X X mann echinocereus) Ferocactus acanthodes...TABLE 3-3 (Cont.) Site Number Species 1 2 3 4 5 6 7 8 9 CACTACEAE (Cont.) Ferocactus sp X X X X *Neolloydia sp. X *-Opuntia basilaris (beavertail X X

  8. Field Surveys, IOC Valleys. Biological Resources Survey, Dry Lake Valley, Nevada. Volume II, Part I.

    Science.gov (United States)

    1981-08-01

    members of the cactaceae family: Coryphantha vivipara, Echinocereus engelmannii, Sclerocactus pubispinus, Opuntia erinacea, Opuntia polycantha, and...Streptantnus cordatus ArFtemisia tr-identata Arem s p. Cactaceae Aster sp. Corypnantha vivipara* Baiy pleniradiata Ectinocereus engelmannii* c W -acis sp

  9. M-X Environmental Technical Report. Environmental Characteristics of Alternative Designated Deployment Areas, Protected Species.

    Science.gov (United States)

    1980-12-22

    Cactaceae and the genus Yucca and all evergreen trees are protected under NRS 527.050 and NRS 527.070. Utah has no state laws which afford protection to...endangered under NRS 527.270 occur in or near geotechnically suitable areas. In addition, all members of the family Cactaceae , all members of the

  10. 米邦塔食用仙人掌丰产株型培育研究%The Cultivation Study for High Productive Stem Type of Edible Cactus Opuntia milpa alta Haw (Cactaceae)

    Institute of Scientific and Technical Information of China (English)

    向青云; 蔡永强; 陈家龙; 王祖泽; 刘晓燕; 邵宇

    2004-01-01

    通过对米邦塔食用仙人掌田间农艺性状调查,建立其掌片重量与纵横径、厚度的回归方程y=(10.408+0.556AB)h,用此模型对不同株型的产量比较,认为株型Ⅲ是生产米邦塔食用仙人掌菜片的最佳株型;株型Ⅱ是培育米邦塔食用仙人掌种片的最佳株型.田间栽培时,应根据生产目的选择适合的株型.

  11. Germinação in vitro e desenvolvimento pós-seminal de plântulas de Pilosocereus aurisetus (Werderm. Byles & G.D. Rowley (Cactaceae

    Directory of Open Access Journals (Sweden)

    Michele Valquíria dos Reis

    2012-12-01

    Full Text Available Pilosocereus aurisetus é uma espécie de cactos de importância econômica e ambiental que se encontra em risco de extinção. A propagação em áreas naturais ocorre, principalmente, de forma sexuada; entretanto, não há registro da germinação e viabilidade de sementes e morfologia pós-seminal de plântulas dessa espécie. Assim, objetivou-se avaliar a germinação de sementes e descrever a morfologia do desenvolvimento pós-seminal de plântulas de P. aurisetus. Para isso, sementes, armazenadas em condições ambientais por 19 meses, foram submetidas aos tratamentos: embebição em água por 24 horas; pré-resfriamento; imersão em solução de giberelina, nas concentrações de 250 mg L-1 e 500 mg L-1; e um tratamento controle. As sementes foram colocadas para germinar em meio de cultura MS, por 30 dias, quando se avaliou a percentagem de germinação. O delineamento estatístico foi o inteiramente casualizado, com cinco tratamentos e quatro repetições, sendo dispostas 25 sementes por parcela. A caracterização pós-seminal foi realizada por um período de 60 dias, utilizando-se microscópio binocular, com base nas Regras para Análise de Sementes. Maior percentagem da germinação de sementes ocorreu no controle, ou quando embebidas por 24 horas, sendo observados 90% e 83%, respectivamente. A morfologia do desenvolvimento pós-seminal indicou que a germinação é do tipo epígea, com hipocótilo de reserva; suas plântulas sofrem modificações na região do colo, para a emissão de raízes, e apresentam cerdas no ápice caulinar, mesmo na fase inicial da expansão cotiledonar. A diferenciação e início da formação das costelas iniciam-se aos 60 dias após a germinação, com o desenvolvimento do epicótilo.

  12. 清流引种食用仙人掌越冬气象条件分析%Analysis of Meteorological Conditions for Over- wintering of Opuntia Milpa alta ( Cactaceae opuntia L) in Qingliu County

    Institute of Scientific and Technical Information of China (English)

    童二发

    2005-01-01

    米邦塔食用仙人掌是我国近几年引入的经济作物,根据食用仙人掌受冻的气候指标,在最低气温对比观测基础上,运用清流县1961-2002年最低气温资料,分析食用仙人掌大棚栽培安全越冬的气象条件,并讨论如何利用山地气候资源提高安全越冬机率,所得结果有益于当地趋利避害发展食用仙人掌.

  13. The Selection for Vegetable Use Irom Wild Type of Cactus Opuntia milpa (Cactaceae)in Guizhou%贵州野生仙人掌的菜用类型筛选

    Institute of Scientific and Technical Information of China (English)

    蔡永强; 向青云; 陈家龙; 王祖泽

    2004-01-01

    以引进种米邦塔食用仙人掌(Opuntia milpa alta,梨果仙人掌黄花品系)外观特征为标准,对贵州野生半野生状态的梨果仙人掌红花品系(Opuntia ficus-indica Mill)等4种仙人掌属的仙人掌植物学形态特征进行比较,并结合当地群众食用记载,初步筛选出形态特征与米邦塔食用仙人掌接近的梨果仙人掌红花品系,野外采集种片至大田驯化栽培后获得成功,并将其农艺性状及嫩茎营养品质进行分析.结果表明,贵州野生的梨果仙人掌红花品系农艺性状较优,主要营养物质含量丰富,作为菜用仙人掌类型开发利用具有较大的前景.

  14. Leaf and stem CO/sub 2/ uptake in the three subfamilies of the Cactaceae. [Pereskia aculeata; Pereskia grandifolia; Maihuenia poeppigii; Carnegiea gigantea; Ferocactus acanthodes; Coryphantha vivipara; Mammillaria dioica; Opuntia ficus-inidica; Pereskiopsis porteri; Quiabentia chacoensis; Austrocylindropuntia subulata

    Energy Technology Data Exchange (ETDEWEB)

    Nobel, P.S.; Hartsock, T.L.

    1986-04-01

    Net CO/sub 2/ uptake over 24-hour periods was examined for the leaves and for the stems of 11 species of cacti representing all three subfamilies. For Pereskia aculeata, Pereskia grandifolia, and Maihuenia poeppigii (subfamily Pereskioideae), all the net shoot CO/sub 2/ uptake was by the leaves and during the daytime. In contrast, for the leafless species Carnegiea gigantea, Ferocactus acanthodes, Coryphantha vivipara, and Mammillaria dioica (subfamily Cactoideae), all the shoot net CO/sub 2/ uptake was by the stems and at night. Similarly, for leafless Opuntia ficus-indica (subfamily Opuntioideae), all net CO/sub 2/ uptake occurred at night. For leafy members of the Opuntioideae (Pereskiopsis porteri, Quiabentia chacoensis, Austrocylindropuntia subulata), at least 88% of the shoot CO/sub 2/ uptake over 24 hours was by the leaves and some CO/sub 2/ uptake occurred at night. Leaves responded to the instantaneous level of photosynthetically active radiation (PAR) during the daytime, as occurs for C/sub 3/ plants, whereas nocturnal CO/sub 2/ uptake by stems of O. ficus-indica and F. acanthodes responded to the total daily PAR, as occurs for Crassulacean acid metabolism (CAM) plants. Thus, under the well-watered conditions employed, the Pereskioideae behaved as C/sub 3/ plants, the Cactoideae behaved as CAM plants, and the Opuntioideae exhibited characteristics of both pathways.

  15. Field Surveys, IOC Valleys. Volumes II-I and II-II. Biological Resources Survey, Dry Lake Valley, Nevada and Pine and Wah Wah Valleys, Utah. Supplement. Spring Survey of the IOC Valleys.

    Science.gov (United States)

    1981-08-01

    pinnata X K CACTACEAE Coryphantha vivipara x Opuntia echinocarpa K 0 x K K K 5putaerinacea K Op’untia sp. x X -12- TABLE 3-1 (Cont.) Shelter site...pilosus x Lepidium montanun X CACTACEAE Opuntia echinocarpa x x Opuntia erinacea X X X CHENOPODIACEAE Atriplex canescens X X X Ceratoides lanata X X X X...Stanleya pinnata X CACTACEAE Coryphantha vivipara var. rosea X Echinocereus engelmannii X opuntia echinocarpa X X X X X X X X Opuntia erinacea XX X X X X x

  16. Efeito de diferentes componentes no meio de cultura na produção de alcalóides em tecidos de calos de Cereus peruvianus (Cactaceae - DOI: 10.4025/actascibiolsci.v27i1.1355 Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae - DOI: 10.4025/actascibiolsci.v27i1.1355

    Directory of Open Access Journals (Sweden)

    Claudete Aparecida Mangolin

    2005-03-01

    Full Text Available Tecidos de calos em cultura prolongada de Cereus peruvianus foram utilizados para investigar a produção de alcalóides pelos calos quando mantidos em meio de cultura suplementados com diferentes concentrações de tirosina, com diferentes níveis de 2,4-D/cinetina, e contendo NaCl. Foi analisado também, o padrão de isozimas α- e β-esterases, para investigar a expressão diferencial de genes nos calos subcultivados com as diferentes concentrações de tirosina. A maior quantidade de alcalóides foi obtida a partir de calos mantidos em meio contendo 200 mg/L de tirosina. A adição de tirosina no meio induziu uma expressão gênica diferencial para a síntese de isozimas α- e β-esterases. Duas α-carboxilesterases (EC 3.1.1.1 e uma α/β-arilesterase (EC 3.1.1.2 foram induzidas nos calos, uma α/β-acetilesterase (EC 3.1.1.6 e uma α-carboxilesterase foram detectadas como bandas mais intensamente coradas, enquanto duas α/β-carboxilesterases não foram detectadas ou foram fracamente coradas após a adição de tirosina como precursor no meio de culturaThe production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1 and one α/β-arylesterase (EC 3.1.1.2 were induced in callus, one α/β-acetylesterase (EC 3.1.1.6 and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursor

  17. Utilização de frutos de cactos (Cactaceae como recurso alimentar por vespas sociais (Hymenoptera, Vespidae, Polistinae em uma área de caatinga (Ipirá, Bahia, Brasil The use of cactus fruit food resources by social wasp (Hymenoptera, Vespidae, Polistinae in an area of Caatinga (lpirá, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Gilberto M. M. Santos

    2007-12-01

    Full Text Available Este estudo teve por objetivo identificar as espécies utilizadas como recurso alimentar e descrever os padrões locais de utilização de frutos de cactos como recurso alimentar por vespas sociais em uma área de Caatinga. Novecentos e oito indivíduos de nove espécies de vespas foram capturados visitando seis espécies de cactáceas. Cereus jamacaru DC. e Pilosocereus catingicola (Gurke Byles & G.D foram as cactáceas mais utilizadas por vespas sociais, tanto em número de espécies quanto de indivíduos. Polybia paulista von Ihering, 1896, Polybia ignobilis (Haliday, 1836, Polistes versicolor (Olivier, 1791, Polistes simillimus Zikán, 1951, Polistes billardieri Fabricius, 1804, Polistes canadensis (Linnaeus, 1758, Polybia occidentalis (Olivier, 1791 e Polybia sericea (Olivier, 1705 apresentaram as maiores amplitudes de nicho trófico. As maiores sobreposições no nicho trófico foram observadas entre Mischocyttarus lanei Zikán, 1949 e Polistes simillimus Zikán, 1951 (0,83. Os altos níveis de sobreposição de nichos observados são explicados em função do comportamento generalista das vespas sociais, bem como da exploração intensiva de poucas espécies de cactáceas.This study aims to identify food resource plants used by social wasp species in a Caatinga area, as well as describe the local patterns of cactus fruit resources used by wasps. Nine hundreds and eight foraging wasps, belonging to nine species, were captured while visiting six cactus species. Cereus jamacaru DC. and Pilosocereus catingicola (Gurke Byles & G.D. were the most frequently visited plants. Several wasp species and a great number of individuals visited them. Polybia paulista von Ihering, 1896, Polybia ignobilis (Haliday, 1836, Polistes versicolor (Olivier, 1791, Polistes simillimus Zikán, 1951, Polistes billardieri Fabricius, 1804, Polistes canadensis (Linnaeus, 1758, Polybia occidentalis (Olivier, 1791 and Polybia sericea (Olivier, 1705 had the larger trophic niche breadth. The highest trophic niche overlap was between Mischocyttarus lanei Zikán, 1949 and Polistes simillimus Zikán, 1951 (0.83. The high niche overlap among social wasps seems to be resulting of the generalist behavior of the social wasp, as well as of the intensive exploration of few cactus species.

  18. The Effect of Plantlet Quality on the Productivity and Agronomic Characteristics of Edible Cactus Opuntia milpa alta Haw (Cactaceae)%米邦塔食用仙人掌种片质量对其菜片产量及农艺性状的影响

    Institute of Scientific and Technical Information of China (English)

    蔡永强; 向青云; 陈家龙; 王祖泽

    2004-01-01

    对米邦塔食用仙人掌不同规格种片进行标记,定植到田间后,定期进行生长动态观测.结果表明,菜片产量与种片纵横径、厚度呈极显著正相关(相关系数分别为0.6702、0.651、0.6281),菜片产量变化幅度较大.根据贵州食用仙人掌生产实际,初步提出贵州省米邦塔食用仙人掌种苗(种片)质量标准为:种片纵径≥24 cm,横径≥15 cm,厚度≥1.5 cm.该项指标可作为贵州食用仙人掌种片质量参考标准.

  19. 78 FR 40673 - Endangered and Threatened Wildlife and Plants; Designation of Critical Habitat for the Acuña...

    Science.gov (United States)

    2013-07-08

    ...--plants. (a) Flowering plants. * * * * * Family Cactaceae: Echinomastus erectocentrus var. acunensis (acu... Fish and Wildlife Service 50 CFR Part 17 RIN 1018-AZ43 Endangered and Threatened Wildlife and Plants... habitat for both plants (77 FR 60509). For the acu a cactus, we proposed to designate as critical...

  20. Phenology of blue cactus moth Melitara prodenialis (Lepidoptera: Pyralidae)

    Science.gov (United States)

    Native cactus plants (Opuntia stricta Haw. [Cactaceae]) were sampled weekly at St. Marks National Wildlife Refuge, St. Marks, Florida (30.16 - 30° 1' N, -84.21 - 84° 1' W) from September 2006 to September 2007 for the native blue cactus moth, Melitara prodenialis Walker (Lepidoptera: Pyralidae) Meli...

  1. Characterization of Salmonella spp. from nopal leaves and associated soil and water samples in Morelos, Mexico

    Science.gov (United States)

    Introduction: Nopal is a native cactus specie [Opuntia ficus-indica (L.) MILL (Cactaceae)] of great economic importance in Mexico. It is grown in open fields and subsequently ingested fresh as a salad or processed as a juice or yogurt, but it may also be used as a dietary supplement and/or for cosme...

  2. Reproductive biology, hybridization, and flower visitors of rare Sclerocactus taxa in Utah's Uintah Basin

    Science.gov (United States)

    The mating system and flower visitors of two threatened species of Sclerocactus (Cactaceae) were studied in the Uintah Basin of eastern Utah, an area undergoing rapid energy development. We found that both S. wetlandicus and S. brevispinus, as well as a third presumptive taxon (undescribed) which w...

  3. Field host range of Apanteles opuntiarum (Hymenoptera: Braconidae) in Argentina, a potential biocontrol agent of Cactoblastis cactorum (Lepidoptera: Pyralidae) in North America

    Science.gov (United States)

    Cactoblastis cactorum (Berg) (Lepidoptera, Pyralidae) was successfully used for biological control of Opuntia spp. (Cactaceae) in Australia and South Africa, where no native cacti occur. Since 1989, this South American moth has been invading the southeastern United States, threatening the unique ca...

  4. Biofilm formation and antimicrobial resistance of Salmonella spp. isolated from nopal stems and water and soil samples

    Science.gov (United States)

    Nopal is a native cactus specie [Opuntia ficus-indica (L.) MILL (Cactaceae)] of appreciable economic importance in Mexico. This plant or its by-products are typically ingested fresh as a salad or processed into juice or yogurt, but it can also be used as a dietary supplement or be formulated into co...

  5. New species of Ashmeadiella Cockerell (Hymenoptera: Megachilidae) from Mexico

    Science.gov (United States)

    Two new species of bees from Mexico are described and illustrated here: Ashmeadiella (Ashmeadiella) danuncia n. sp. and A. (A.) mandibularis n. sp. These species are most frequently found visiting flowers of Cactaceae, although they have also been collected on flowers of Acacia, Prosopis, Lopezia, D...

  6. Beyond aridification: multiple explanations for the elevated diversification of cacti in the New World Succulent Biome.

    Science.gov (United States)

    Hernández-Hernández, Tania; Brown, Joseph W; Schlumpberger, Boris O; Eguiarte, Luis E; Magallón, Susana

    2014-06-01

    Succulent plants are widely distributed, reaching their highest diversity in arid and semi-arid regions. Their origin and diversification is thought to be associated with a global expansion of aridity. We test this hypothesis by investigating the tempo and pattern of Cactaceae diversification. Our results contribute to the understanding of the evolution of New World Succulent Biomes. We use the most taxonomically complete dataset currently available for Cactaceae. We estimate divergence times and utilize Bayesian and maximum likelihood methods that account for nonrandom taxonomic sampling, possible extinction scenarios and phylogenetic uncertainty to analyze diversification rates, and evolution of growth form and pollination syndrome. Cactaceae originated shortly after the Eocene-Oligocene global drop in CO2 , and radiation of its richest genera coincided with the expansion of aridity in North America during the late Miocene. A significant correlation between growth form and pollination syndrome was found, as well as a clear state dependence between diversification rate, and pollination and growth-form evolution. This study suggests a complex picture underlying the diversification of Cactaceae. It not only responded to the availability of new niches resulting from aridification, but also to the correlated evolution of novel growth forms and reproductive strategies.

  7. Review

    NARCIS (Netherlands)

    Heel, van W.A.

    1972-01-01

    This book is an exploration into the field of Plant Morphology. It deals with the placentation of the ovules in ten families of Centrospermae — including the Cactaceae — and in the Primulaceae. The core is formed by a very close observation and a complete documentation of the histogenesis of the ova

  8. Phenology and egg production of the cactus moth, Cactoblastis cactorum(Lepidoptera: Pyralidae): comparison of field census data and life stage development in the field

    Science.gov (United States)

    Natural phenology and development of the cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae) was studied under field conditions in St. Marks National Wildlife Refuge, St. Marks, FL. from July 2006 to September 2007. Cactus pads (Opuntia stricta Haw. [Cactaceae]) were visually surveyed...

  9. Yield and fruit quality traits of dragon fruit lines and cultivars grown in Puerto Rico

    Science.gov (United States)

    Dragon fruit or pitahaya (Hylocereus undatus and Selenicereus megalanthus) is a member of the Cactaceae family and native to the tropical forest regions of Mexico, Central, and South America. The fruit was practically unknown 15 years ago but it occupies a growing niche in Europe’s exotic fruit mar...

  10. Notes on cereus and acanthocereus

    OpenAIRE

    Croizat, Léon

    2012-01-01

    It is commonly believed that the greatest single difficulty of the classification of the Cactaceae is in the fact that many names were published in this family for which types are not extant. This difficulty is great, no doubt, but less fundamental than it seems.

  11. Crystal morphology and carbon/carbon composition of solid oxalate in cacti.

    Science.gov (United States)

    Rivera, E R; Smith, B N

    1979-12-01

    Morphology, crystal structure, and carbon isotopic composition of calcium oxalate from representative species from the family Cactaceae were determined using scanning electron microscopy, x-ray diffraction, and isotope ratio mass spectrometry. Crystals from one species in the Opuntieae tribe of the Cactaceae were druses with acute points composed of the monohydrate form of calcium oxalate (whewellite). Crystals from three species in the Cereeae tribe were the dihydrate form of calcium oxalate (weddellite) forming druses made up of tetragonal and isodiametric crystallites. The oxalate was relatively enriched in (13)C isotope (-7.3 to - 8.7 per thousand) compared with woody fibers (-13.3 to 14.1 per thousand) from the same plants.

  12. Crystal Morphology and 13Carbon/12Carbon Composition of Solid Oxalate in Cacti 1

    Science.gov (United States)

    Rivera, E. R.; Smith, B. N.

    1979-01-01

    Morphology, crystal structure, and carbon isotopic composition of calcium oxalate from representative species from the family Cactaceae were determined using scanning electron microscopy, x-ray diffraction, and isotope ratio mass spectrometry. Crystals from one species in the Opuntieae tribe of the Cactaceae were druses with acute points composed of the monohydrate form of calcium oxalate (whewellite). Crystals from three species in the Cereeae tribe were the dihydrate form of calcium oxalate (weddellite) forming druses made up of tetragonal and isodiametric crystallites. The oxalate was relatively enriched in 13C isotope (-7.3 to - 8.7 ‰) compared with woody fibers (-13.3 to 14.1 ‰) from the same plants. Images PMID:16661115

  13. Wound healing properties and mucilage content of Pereskia aculeata from different substrates

    OpenAIRE

    Eber Goulart Carvalho; Cristina Pacheco Soares; Lorena Blau; Renato Farina Menegon; Walderez Moreira Joaquim

    2014-01-01

    Physiologic growth parameters Wound healing Pereskia aculeata Mill., Cactaceae, is a cactus with high mucilage production, well-known for its nutritional properties. Folk use consists on skin injuries, and mucilage is probably involved in the wound healing activity. This work studied some aspects of its cultivation, specifically regarding soil (substrate), to correlate the effects of nutritional content to mucilage production and to the wound-healing property. Plants were grown under five dif...

  14. Pharmacognostical and phytochemical evaluation of Cactus grandiflorus (L.) Britton and Rose

    OpenAIRE

    Rajat Rashmi; Divya Mishra

    2016-01-01

    Background: Cactus grandiflorus (L.) Britton and Rose, Family: Cactaceae is an evergreen shrub with creeping aerial roots, used in Homoeopathy for atheromatous arteries, angina pectoris, and constriction of heart muscles, endocarditis, and heart weakness due to arteriosclerosis. Flowering stems are used in the preparation of medicine. Objective: The pharmacognostic and phytochemical studies are carried out to facilitate identification of correct species and standardized raw materials. Materia...

  15. 6-Hydroxy-methyl-4-meth-oxy-2H-pyran-2-one (Opuntiol).

    Science.gov (United States)

    Abbasi, Muhammad Athar; Shahzadi, Tayyaba; Akkurt, Mehmet; Aziz-Ur-Rehman; Riaz, Tauheeda

    2009-12-19

    The title compound, C(7)H(8)O(4), isolated from Opuntia dillenii Haw (Cacta-ceae), is almost planar [maximum deviation of 0.027 (2) Å] except for the H atoms of the methylene and methyl groups. The crystal packing is stabilized by C-H⋯O and O-H⋯O inter-molecular hydrogen bonds, resulting in the formation of a three-dimensional network.

  16. Acute oral toxicity of Pereskia bleo and Pereskia grandifolia in mice

    OpenAIRE

    Sim, K. S.; A M Sri Nurestri; Sinniah, S. K.; Kim, K. H.; Norhanom, A. W.

    2010-01-01

    Pereskia bleo and Pereskia grandifolia, belonging to the botanical family Cactaceae, have been traditionally used by the locals in Malaysia for treatment of various ailments. The current study reports the outcome of acute oral toxicity investigation of Pereskia bleo and Pereskia grandifolia, on ICR mice. No mortalities or evidence of adverse effects have been observed in ICR mice following acute oral administration at the highest dose of 2500 mg/ kg crude extracts of Pereskia bleo and Pereski...

  17. 6-Hydroxymethyl-4-methoxy-2H-pyran-2-one (Opuntiol

    Directory of Open Access Journals (Sweden)

    Muhammad Athar Abbasi

    2010-01-01

    Full Text Available The title compound, C7H8O4, isolated from Opuntia dillenii Haw (Cactaceae, is almost planar [maximum deviation of 0.027 (2 Å] except for the H atoms of the methylene and methyl groups. The crystal packing is stabilized by C—H...O and O—H...O intermolecular hydrogen bonds, resulting in the formation of a three-dimensional network.

  18. New distribution record of Cybocephalus kathrynae (Coleoptera, Cybocephalidae on Mona Island, Puerto Rico

    Directory of Open Access Journals (Sweden)

    Jean Carlos Curbelo-Rodríguez

    2012-03-01

    Full Text Available New distribution record of Cybocephalus Kathrynae (Coleoptera, Cybocephalidae on Mona Island, Puerto Rico. A new record of Cybocephalus kathrynae T.R. Smith (Cybocephalidae is reported for Puerto Rico. Adults were collected from the flowers of Mammillaria nivosa (Cactaceae on Mona Island Reserve. Prior to this study, this beetle species was only reported for Monroe and Miami-Dade Counties, Florida, USA.

  19. 6-Hydroxy­methyl-4-meth­oxy-2H-pyran-2-one (Opuntiol)

    OpenAIRE

    Muhammad Athar Abbasi; Tayyaba Shahzadi; Mehmet Akkurt; Aziz-ur-Rehman,; Tauheeda Riaz

    2009-01-01

    The title compound, C7H8O4, isolated from Opuntia dillenii Haw (Cactaceae), is almost planar [maximum deviation of 0.027 (2) Å] except for the H atoms of the methylene and methyl groups. The crystal packing is stabilized by C—H...O and O—H...O intermolecular hydrogen bonds, resulting in the formation of a three-dimensional network.

  20. Karakterisasi Simplisia dan Skrining Fitokimia serta Uji Aktivitas Antioksidan Ekstrak Etanol Buah Naga (Hylocereus undatus (Haw.) Britton & Rose)

    OpenAIRE

    Siregar, Nur Khaidah

    2011-01-01

    The dragon fruit plant (Hylocereus undatus (Haw.) Britton & Rose) family of cactaceae has been cultivated at Indonesia and increasingly popular for the community. Unique and interesting fruit shape, red of shell and green turtle similar to the turtle’s of dragon, it was sweet, sour and fresh, contain chemical compounds such as vitamin C, vitamin A vitamin E and polyphenols that can lowered blood pressure, cholesterol and blood glucose level, it also prevent spreading of cancer cell. In t...

  1. Lignans from Opuntia ficus-indica seeds protect rat primary hepatocytes and HepG2 cells against ethanol-induced oxidative stress.

    Science.gov (United States)

    Kim, Jung Wha; Yang, Heejung; Kim, Hyeon Woo; Kim, Hong Pyo; Sung, Sang Hyun

    2017-01-01

    Bioactivity-guided isolation of Opuntia ficus-indica (Cactaceae) seeds against ethanol-treated primary rat hepatocytes yielded six lignan compounds. Among the isolates, furofuran lignans 4-6, significantly protected rat hepatocytes against ethanol-induced oxidative stress by reducing intracellular reactive oxygen species levels, preserving antioxidative defense enzyme activities, and maintaining the glutathione content. Moreover, 4 dose-dependently induced the heme oxygenase-1 expression in HepG2 cells.

  2. Characterization of calcium oxalates generated as biominerals in cacti.

    Science.gov (United States)

    Monje, Paula V; Baran, Enrique J

    2002-02-01

    The chemical composition and morphology of solid material isolated from various Cactaceae species have been analyzed. All of the tested specimens deposited high-purity calcium oxalate crystals in their succulent modified stems. These deposits occurred most frequently as round-shaped druses that sometimes coexist with abundant crystal sand in the tissue. The biominerals were identified either as CaC(2)O(4).2H(2)O (weddellite) or as CaC(2)O(4).H(2)O (whewellite). Seven different species from the Opuntioideae subfamily showed the presence of whewellite, and an equal number of species from the Cereoideae subfamily showed the deposition of weddellite. The chemical nature of these deposits was assessed by infrared spectroscopy. The crystal morphology of the crystals was visualized by both conventional light and scanning electron microscopy. Weddellite druses were made up of tetragonal crystallites, whereas those from whewellite were most often recognized by their acute points and general star-like shape. These studies clearly demonstrated that members from the main traditional subfamilies of the Cactaceae family could synthesize different chemical forms of calcium oxalate, suggesting a definite but different genetic control. The direct relationship established between a given Cactaceae species and a definite calcium oxalate biomineral seems to be a useful tool for plant identification and chemotaxonomy.

  3. Characterization of Calcium Oxalates Generated as Biominerals in Cacti1

    Science.gov (United States)

    Monje, Paula V.; Baran, Enrique J.

    2002-01-01

    The chemical composition and morphology of solid material isolated from various Cactaceae species have been analyzed. All of the tested specimens deposited high-purity calcium oxalate crystals in their succulent modified stems. These deposits occurred most frequently as round-shaped druses that sometimes coexist with abundant crystal sand in the tissue. The biominerals were identified either as CaC2O4.2H2O (weddellite) or as CaC2O4.H2O (whewellite). Seven different species from the Opuntioideae subfamily showed the presence of whewellite, and an equal number of species from the Cereoideae subfamily showed the deposition of weddellite. The chemical nature of these deposits was assessed by infrared spectroscopy. The crystal morphology of the crystals was visualized by both conventional light and scanning electron microscopy. Weddellite druses were made up of tetragonal crystallites, whereas those from whewellite were most often recognized by their acute points and general star-like shape. These studies clearly demonstrated that members from the main traditional subfamilies of the Cactaceae family could synthesize different chemical forms of calcium oxalate, suggesting a definite but different genetic control. The direct relationship established between a given Cactaceae species and a definite calcium oxalate biomineral seems to be a useful tool for plant identification and chemotaxonomy. PMID:11842173

  4. Raman spectroscopy study of calcium oxalate extracted from cacti stems.

    Science.gov (United States)

    Frausto-Reyes, Claudio; Loza-Cornejo, Sofia; Terrazas, Teresa; Terrazas, Tania; Miranda-Beltrán, María de la Luz; Aparicio-Fernández, Xóchitl; López-Macías, Brenda M; Morales-Martínez, Sandra E; Ortiz-Morales, Martín

    2014-01-01

    To find markers that distinguish the different Cactaceae species, by using near infrared Raman spectroscopy and scanning electron microscopy, we studied the occurrence, in the stem, of solid deposits in five Cactaceae species (Coryphantha clavata, Ferocactus latispinus, Opuntia ficus-indica, O. robusta, and O. strepthacantha) collected from their natural habitats from a region of México. The deposits in the tissues usually occurred as spheroidal aggregates, druses, or prismatic crystals. From the Raman spectra, the crystals were identified either as calcium oxalate monohydrate (CaC2O4·H2O) or calcium oxalate dihydrate (CaC2O4·2H2O). Opuntia species (subfamily Opuntioideae) showed the presence of CaC2O4·H2O, and the deposition of CaC2O4·2H2O was present in C. clavata and F. latispinus (subfamily Cactoideae, Cacteae tribe). As a punctual technique, Raman spectroscopy seems to be a useful tool to identify crystal composition. In addition to allowing the analysis of crystal morphology, this spectroscopic technique can be used to identify Cactaceae species and their chemotaxonomy.

  5. Flora cactológica y especies asociadas en el área natural protegida Sierra Corral de los Bandidos, Nuevo León, México Cactus list and asociated plants of the protected natural area Sierra Corral de Los Bandidos, Nuevo Leon, Mexico

    Directory of Open Access Journals (Sweden)

    María del Pilar Carmona-Lara

    2008-12-01

    Full Text Available El área natural protegida (ANP Sierra Corral de los Bandidos ubicada en la sierra Madre Oriental, al noroeste de Monterrey, sufre el impacto de actividades antropológicas asociadas al matorral submontano (ganadería, agricultura, colecta y urbanismo, disminuyendo sus recursos naturales y amenazando sus especies endémicas, principalmente cactáceas. El propósito del estudio fue conocer la diversidad de cactáceas y especies asociadas en zonas de amortiguamiento y núcleo. Para ello se determinaron índices de riqueza y similitud utilizando un muestreo estratificado con cuadrantes a lo largo de transectos orientados por gradientes altitudinales según su zonificación. En total se registraron 112 taxa (30 cactáceas, 87 (24 cactáceas en zona de amortiguamiento y 80 (19 cactáceas en zona núcleo. Por su densidad, frecuencia y abundancia sobresalieron Echinocereus stramineus (Engelm. Engelm. ex F. Seitz, Cylindropuntia leptocaulis F. M. Knuth in Backeb et F. M. Knuth, Mammillaria melanocentra Poselg., Neolloydia conoidea (DC Britton et Rose, (Cactaceae; Erioneuron avenaceum (H. B et K. Tateoka, (Poaceae; Viguiera stenoloba S. F. Blake; Zexmenia hispida (Kunth A. Gray, (Asteraceae y Agave lechuguilla Torr, (Agavaceae. Existe diferencia significativa entre las poblaciones de cactus de las zonas del ANP, según Jacquard (13%, Sörensen (38% y Morisita (0.44. Ocho cactáceas (7 endémicas están registradas en algún estatus de la NOM-ECOL-059, 2001.The Sierra Corral de los Bandidos is a natural protected area located in the Sierra Madre Oriental mountain range northwest of Monterrey city. This preserve suffers the impact of human activities in the form of cattle ranching, agriculture, harvest of wild plants, and urbanism, diminishing its natural resources and threatening its endemic species, especially from the cactaceae family. The purpose of this study was to obtain information on the diversity of cacti and associated species in the buffer and

  6. Морфологические и генетические особенности видов рода Rebutia K. Schum. семейства cactaceae JUSS. и их гибридных растений F1

    Directory of Open Access Journals (Sweden)

    Елена Сергеевна Чичканова

    2015-08-01

    Full Text Available Выявлены параметры – диаметр и высота побегов, длина корня, количество колючек у Rebutia senilis, Rebutia marsoneri и ♀ Rebutia senilis × ♂ Rebutia  marsoneri в F1, которые возможно применять при дифференциации в онтогенезе. Установлена возможность применения ISSR маркеров для генетической характеристики родительских форм R. senilis, R. marsoneri, их гибридных сеянцев

  7. The morphology and systematic position of the Didiereaceae of Madagascar

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    W. Rauh

    1983-12-01

    Full Text Available The Didiereaceae, endemic to the arid south-west of Madagascar is one of the most remarkable plant families in the world. Four genera and 11 species are known, of which 3 have been described by the author:  Decaryia (1 species, Alluaudiopsis (2 spp., Alluaudia (6 spp. and Didierea (2 spp.. The species show different growth forms, but their shoot-stem-systems are basically similar; they are all spiny, semi-succulent deciduous shrubs or trees, with a stem (shoot system differentiated into long-shoots and short-shoots (brachyblasts. The long shoots bear normal leaflets, which soon fall off. In their axils, brachyblasts develop proleptically, which produce two types of leaves (1 thorns which develop proleptically in different numbers (1 in Alluaudia, 2 in Alluaudiopsis marnieriana and several Didierea-, (2 leaves which are green, deciduous assimilation organs (2 per brachyblast in Alluaudia, several in Didierea. The stem organization is comparable to that of the Cactaceae of the New World. The brachyblasts of the Didiereaceae are homologous to the areoles of the Cactaceae, most obviously to those of the primitive members, the Pereskioideae. The affinities between the two families can be demonstrated by means of grafting. It is possible to graft Didiereas onto Pereskioideae and vice versa. The flower structure of Didiereaceae is very uniform. Flowers are unisexual and dioecious and show affinities to those of the Portulacaceae. The development of the gynoecium, the ovule and the female gametophyte as well as the presence of betalains in the flowers indicate that the Didiereaceae do not belong to the Sapindales as was sometimes assumed, but to the Centrospermae. They should be placed between Portulacaceae and Cactaceae. The ancestors of the Didiereaceae are not known.

  8. RESEARCH CONCERNING THE CHEMICAL CONTROL AGAINST PSEUDOCOCCUS ADONIDUM L.

    Directory of Open Access Journals (Sweden)

    Maria ECOBICI

    2005-01-01

    Full Text Available Pseudococcus adonidum is a polyphag pest, which attacks different species of plants belonging to many genus and families, as: Citrus, Ficus, Cactaceae, Cycas, Dracena, Phoenix, Mimoza, Asparagus, Camellia etc. It is localized in large colonies on leaves, stems, branches, offshoots, and roots. The experiments were performed in solarium conditions during 2001-2002. Testing the efficiency of an insecticide sort was the aim of our experiment. During 2 experimental years, 9 products were tested: Confidor 200 SL, Calypso 400 SC; Pyrinex 25 EC, Vydate 10 G, Fastac 10 CE – RV, Sumithion 50 EC, Mospilan 20 SP, Decis 2,5 EC, Actellic 50 EC.

  9. Experimental Study on the Antimutagenesis Function of Opuntia dillenil Haw%仙人掌抗诱变效应的实验研究

    Institute of Scientific and Technical Information of China (English)

    王桂秋; 聂晶; 朱黎霞; 刁恩英

    2001-01-01

    @@ 仙人掌[Opuntia dillenii(Ker-Gaw)Haw]为石竹目(Caryophllales)仙人掌科(Cactaceae)植物.以全株入药,具有行气活血、祛湿退热、生肌等作用[1].近年来有许多报道证实了仙人掌的化学成分和药理作用[2].而有关仙人掌的遗传毒理和抗环磷酰胺诱变的研究尚未见报道.

  10. Compostos bioativos de Opuntia ficus indica

    OpenAIRE

    Semedo, Ana Catarina Jorge

    2012-01-01

    Tese de mestrado, Controlo da Qualidade e Toxicologia dos Alimentos, Universidade de Lisboa, Faculdade de Farmácia, 2012 A espécie Opuntia, da família Cactaceae, originária das altas montanhas vulcânicas do México (Centro e Sul), tem vindo a ser cada vez mais, alvo de estudos. Pensa-se que os compostos fenólicos já identificados nesta espécie podem ser responsáveis pela atividade antioxidante, anti-inflamatória, hipoglicemiante, anticancerigena e neuroprotectiva, entre outras, atribuídas a...

  11. SASTOJCI KATUSOVOG PLODA (OPUNTIA FICUS INDICA) INKORPORIRANI U DIJETETSKE PROIZVODE KOJI SE KORISTE KAO SUPLEMENTI HRANI ILI OTC

    OpenAIRE

    Blazevska, Zagorka; Cvrk, Ramzija; Aleksovski, Aleksandar

    2012-01-01

    Opuntia ficus indica je vrsta kaktusa koja pripada familiji Cactaceae. Ova familija kaktusa obuhvata oko 200 podvrsta kaktusa koji su rasprostranjeni najviše u Americi. Najčešću kulinarsku primjenu ima Indijska Fig Opuntia (Opuntia ficus indica ), jer se njeni listovi i plodovi koriste kao svježe salate. Plod kaktusa se konzumira zreo u ljetnom periodu. Najviše su rasprostranjeni na zapadnoj hemisferi (Meksiko) i zapadnim dijelovima Amerike, ali mogu se naći i u drugim dijelovima svijeta (Gal...

  12. The closest relatives of cacti: insights from phylogenetic analyses of chloroplast and mitochondrial sequences with special emphasis on relationships in the tribe Anacampseroteae.

    Science.gov (United States)

    Nyffeler, Reto

    2007-01-01

    Recent molecular and morphological systematic investigations revealed that the cacti are most closely related to Anacampseroteae, Portulaca and Talinum of the family Portulacaceae (ACPT clade of suborder Portulacineae). A combined analysis of ndhF, matK, and nad1 sequence data from the chloroplast and the mitochondrial genomes indicates that the tribe Anacampseroteae is the sister group of the family Cactaceae. This clade, together with Portulaca, is well characterized by the presence of axillary hairs or scales. Relationships within Anacampseroteae are characterized by a grade of five species of Grahamia s.l. from North and South America, and Grahamia australiana is found to be sister to the genera Anacampseros and Avonia. A comparison of vegetative characteristics indicates an evolutionary transition from woody subshrubs to dwarf perennial and highly succulent herbs during the diversification of Anacampseroteae. Available evidence from the present investigation as well as from previously published studies suggests that a revised classification of Portulacineae on the basis of inferred phylogenetic relationships might consist of a superfamily that includes Cactaceae and the three genera Anacampseros s.l. (including Avonia and Grahamia s.l.), Portulaca, and Talinum (including Talinella), either referred to three monogeneric families or to a paraphyletic family Portulacaceae*.

  13. Dieta de murciélagos nectarívoros del Parque Nacional Cerros de Amotape, Tumbes

    Directory of Open Access Journals (Sweden)

    Edith Arias

    2011-07-01

    Full Text Available En el Perú se reporta la presencia de 18 especies de murciélagos nectarívoros, sin embargo se cuenta con poca información sobre la dieta de estas especies. En este estudio se reporta por primera vez la dieta de los nectarívoros Glossophaga soricina, Lonchophylla hesperia y Anoura geoffroyi en el bosque seco ecuatorial y del bosque tropical del Pacífico del Parque Nacional Cerros de Amotape, Tumbes. Analizamos 21 contenidos gastrointestinales e identificamos ocho morfotipos de polen pertenecientes a las familias Bombacaceae, Cactaceae, Fabaceae, Solanaceae, Rubiaceae, Myrtaceae, Malvaceae y Rosaceae. Encontramos evidencia del síndrome de quiropterofilia en Bombacaceae, Cactaceae, Fabaceae, Solanaceae y Rubiaceae. Observamos que A. geoffroyi consume polen de Ceiba trichistandra, Solanaceae y Rubiacea; G. soricina consume de Abutilon reflexum, Armathocereus cartwrightianus, C. trichistandra y Rubiaceae; y L. hesperia de A. cartwrightianus, Eriobotrya japonica, Fabaceae y Psidium sp.; sugiriendo una dieta generalista en estas especies. Los murciélagos G. soricina y A. geoffroyi comparten el consumo del ceibo C. trichistandra y de la Rubiaceae, mientras que G. soricina comparte con L. hesperia el consumo del cactus A. cartwrightianus. Los otros morfotipos de polen no fueron compartidos entre murciélagos. Se encuentra además que el ceibo C. trichistandra fue la especie más consumida, especialmente por G. soricina.

  14. EFEITOS DO PASTEJO DESCONTROLADO SOBRE A FITOCENOSE DE DUAS ÁREAS DO CARIRI ORIENTAL PARAIBANO

    Directory of Open Access Journals (Sweden)

    Rômulo Gil de Luna

    2007-01-01

    Full Text Available A phytosociological comparative study was performed in two "caatinga" areas (A - productive pasture and B - degraded pasture, in the eastern "Cariri" microregion of Paraíba State, Northeast of Brazil. The multiple plots method was used, where 40 plots were established in each area, being measured living trees and shrubs with diameter at base height (DAB ³ 3 cm and plant height (At ³ 1 m. Eight botanic families, ten genera, and ten species were identified in area A. The Cactaceae and Mimosaceae were the best-represented families in number of individuals in this area. Six families, eight genera, and nine species were identified in area B, where Cactaceae, Mimosaceae and Euphorbiaceae were the best-represented families. The following parameters, total number of sampled individuals (N, basal area (BA, and total density (TD had in area A 438 individuals, 12.22 m2.ha-1, and 2737 individuals per hectare, respectively, which were higher than area in B, that had the following values: 308 individuals, 11.31 m2.ha-1 e 1925 individuals per hectare, respectively. These results allow to postulate that the uncontrolled grazing practiced in that microregion of Paraíba State, is leading to an environmental degradation of the "caatinga" phytocenosis (a set of plants with ecological similar preferences, living in the same biotope.

  15. Patterns of species richness and abundance among cactus communities receiving different rainfall levels in the semiarid region of Brazil

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio Monteiro Ferreira

    Full Text Available ABSTRACT This study examines the variation in cacti species richness and abundance among sites with different average rainfall and soil types. We assessed a total of 3,660 individuals of six species of Cactaceae: Cereus jamacaru, Melocactus zehntneri, Pilosocereus gounellei, Pilosocereus pachycladus, Tacinga inamoena, and Tacinga palmadora. The greatest species richness and abundance of cacti were at locations with low rainfall and more clayey soils. The species studied differed in multidimensional representation, with some species being more positively related to soils with a higher proportion of fine particles (M. zehntneri and P. gounellei, while others were negatively related to soils with a higher proportion of coarser particles (T. inamoena or positively related to areas with higher rainfall and vegetation cover (C. jamacaru and P. pachycladus. The differential responses of the species of Cactaceae studied in relation to the gradients analyzed demonstrates the need for more research into the relationship between cacti and environmental variables in semiarid ecosystems with high environmental heterogeneity.

  16. Antioxidant and antitopoisomerase activities in plant extracts of some Colombian flora from La Marcada Natural Regional Park.

    Science.gov (United States)

    Niño, Jaime; Correa, Yaned Milena; Cardona, Germán David; Mosquera, Oscar Marino

    2011-09-01

    Many plants have been used to treat some diseases and infections since time immemorial, and this potential has been exploited by the pharmaceutical industry in the search of new analgesic, anticarcinogenic and antimicrobial agents, among other active agents. In order to contribute with bioprospection studies on the Colombian flora, 35 extracts from 13 plant species belonging to seven families (Apocynaceae, Cactaceae, Costaceae, Eremolepidaceae, Passifloraceae, Solanaceae and Urticaceae) were collected from La Marcada Natural Regional Park (LMNRP), Colombia. Dichloromethane, n-hexane and aqueous-methanol crude extracts were prepared and evaluated for their activity against Saccharomyces cerevisiae RS322N, R52Y and RS321 strains in the yeast mutant assay and their antioxidant capacity through the DPPH test. The dichloromethane extract from Myriocarpa stipitata (Urticaceae) showed moderate inhibitory activity against the three S. cerevisiae strains tested. The capacity of the dichloromethane extract from M. stipitata to inhibit the enzyme topoisomerase I and to cause DNA damage was inferred from these results. In the DPPH assay, the n-hexane crude extract from Costus sp. (Costaceae) showed good antioxidant activity (48%); in addition, the crude dichloromethane and aqueous-methanol extracts from Rhipsalis micrantha (Cactaceae) showed moderate antioxidant activity with percentage of 29 and 21%, respectively.

  17. Complex Biochemistry and Biotechnological Production of Betalains

    Directory of Open Access Journals (Sweden)

    Marijana Krsnik-Rasol

    2011-01-01

    Full Text Available The demand for natural food colourants is increasing because of public awareness of their health benefits. Betalains are nitrogen-containing plant pigments whose colours range from red-violet betacyanins to yellow betaxanthins. They are used for colouring dairy products, meat and frozen desserts. Betalains have attracted additional interest because of their antioxidative, anti-inflammatory and anticarcinogenic properties. The main source of commercially produced betalains is red beet root, but alternative sources are found in plants from the Amaranthaceae and Cactaceae families. Another alternative source is plant cell culture in bioreactors, although optimization of pigment production seems necessary. In this paper we synthesize the results of recent studies on betalain biosynthesis, chemical properties, sources, biotechnology and applications.

  18. Characterization of arabinogalactan-rich mucilage from Cereus triangularis cladodes.

    Science.gov (United States)

    Petera, B; Delattre, C; Pierre, G; Wadouachi, A; Elboutachfaiti, R; Engel, E; Poughon, L; Michaud, P; Fenoradosoa, T A

    2015-01-01

    Cereus triangularis (Cactaceae) is a cactus used in food decoction as a traditional medicine in the North region of Madagascar to reduce stomach ache and intestinal diseases. Hydrocolloids were sequentially extracted from its cladodes with a yield of 24% (240 mg/g based on dried cladodes powder). Structural analyses has revealed that this polysaccharide with a molecular mass of 8430,000g/mol was mainly composed of a galactan backbone of a (1 → 4) linked β-d-Galp residues probably substituted at position 3 by L-arabinofuranosyl residues. In vitro antioxidant activity of this arabinogalactan-rich fraction was detected and quantified by radical DPPH scavenging, hydroxyl radical scavenging, radical anion superoxide scavenging and reducing power method.

  19. Síndromes de dispersão de Angiospermas em uma Unidade de Conservação na Caatinga, SE, Brasil

    OpenAIRE

    2013-01-01

    O presente estudo teve por objetivos caracterizar os tipos de frutos e as síndromes de dispersão de Angiospermas no Monumento Natural Grota do Angico, localizado nos municípios de Canindé de São Francisco e Poço Redondo, SE, Brasil. A caracterização dos frutos e das síndromes foi baseada na análise do material coletado em uma área de 251 ha. Foram amostradas 67 espécies e 24 famílias, das quais Fabaceae, Euphorbiaceae, Bromeliaceae e Cactaceae, foram as mais representativas. Houve o predomíni...

  20. Evaluación del riesgo de extinción de Mammillaria pectinifera, cactácea endémica de la región de Tehuacán-Cuicatlán

    OpenAIRE

    Pedro Luis Valverde; José Alejandro Zavala-Hurtado; Cecilia Jiménez-Sierra; Beatriz Rendón-Aguilar; Amelia Cornejo-Romero; Sombra Rivas-Arancibia; Gerardo López-Ortega; Marco Aurelio Pérez-Hernández

    2009-01-01

    Mammillaria pectinifera (Cactaceae) es una especie endémica de la Región de Tehuacán Cuicatlán en los estados de Pueblay Oaxaca. Aunque en la Norma Oficial Mexicana NOM-059-ECOL-2001 está considerada como amenazada y está incluida en el Apéndice I de la CITES, la información derivada de los escasos estudios ecológicos sobre esta especie revela serios riesgos de extinción de sus poblaciones. En el presente estudio se registra una evaluación del riesgo de extinción de M. pectinifera, para la q...

  1. Florística e fitogeografia da vegetação arbustiva subcaducifólia da Chapada de São José, Buíque, PE, Brasil Floristics and phytogeography of semideciduous vegetation on the São José plateau, Buíque, Pernambuco State, Brazil

    Directory of Open Access Journals (Sweden)

    Ana Paula de Souza Gomes

    2006-03-01

    Full Text Available Foi realizado o levantamento da flora angiospérmica de um trecho de vegetação arbustiva subcaducifólia na Chapada de São José, Buíque, Pernambuco, com a finalidade de ampliar o conhecimento sobre a flora daquela chapada, bem como compreender suas relações florísticas com outros conjuntos vegetacionais do Nordeste, especialmente no semi-árido. A flora angiospérmica foi composta por 192 táxons, distribuídos em 130 gêneros e 60 famílias. Euphorbiaceae, Caesalpiniaceae, Myrtaceae, Mimosaceae, Fabaceae e Cactaceae foram as mais representativas em número de espécies. A análise de agrupamento revelou que o tipo de substrato exerce uma forte influência na repartição espacial das espécies dentro do semi-árido e confirmou a existência de um conjunto de espécies indicadoras das áreas sedimentares, formado por Caesalpinia microphylla Mart. (Caesalpiniaceae, Bocoa mollis (Benth. R.S. Cowan (Fabaceae, Byrsonima gardneriana A. Juss. (Malpighiaceae e Zanthoxylum stelligerum Turic. (Rutaceae. A flora da área de estudo é relacionada com a da caatinga do cristalino, caatinga de áreas sedimentares e carrasco. Todavia, o alto número de espécies de Myrtaceae, raras na caatinga, o carácter subcaducifólio da vegetação e a presença de Cactaceae e Bromeliaceae, típicas da caatinga, sugerem que a área de estudo representa o final de um gradiente que se inicia em áreas sedimentares situadas em menores altitudes.A floristic survey was carried out in a semideciduous vegetation on São José plateau with the objective of amplifying the knowledge of its angiospermic flora and establishing its relationship with other vegetations types in the semi-arid region of Northeast Brazil. The flora was composed by 192 taxa belonging to 130 genera and 60 families. Euphorbiaceae, Caesalpiniaceae, Myrtaceae, Mimosaceae Fabaceae and Cactaceae were the families with the highest numbers of species. A cluster analysis indicated influence of the soil type

  2. Cactus (Opuntia dillenii Grahm) stem: a new source of energy

    Energy Technology Data Exchange (ETDEWEB)

    Deshpande, V.K. (Dept. of Applied Physics, V.R.C.E., Nagpur (India)); Joshi, A.M. (Dept. of Applied Physics, V.R.C.E., Nagpur (India))

    1994-01-01

    An electrochemical cell has been fabricated using cactus stem as an electrolyte. The electrolyte was a stem (phylloclade) of Opuntia dillenii from the family Cactaceae. A thin slice (1 x 1 x 0.5 cm) of the stem was cut and sandwiched between copper and zinc electrodes. A study of the discharge characteristics reveals that, at a current drain of 100 [mu]A, the cell gives an optimum energy density of 175 mWh kg[sup -1]. The power generated by these cells is sufficient to run piezoelectric buzzer and a LCD calculator for a few hours. This work opens up a new interdisciplinary area for physicists, botanists and electrochemists. (orig.)

  3. Plant hydrocarbon recovery process

    Energy Technology Data Exchange (ETDEWEB)

    Dzadzic, P.M.; Price, M.C.; Shih, C.J.; Weil, T.A.

    1982-01-26

    A process for production and recovery of hydrocarbons from hydrocarbon-containing whole plants in a form suitable for use as chemical feedstocks or as hydrocarbon energy sources which process comprises: (A) pulverizing by grinding or chopping hydrocarbon-containing whole plants selected from the group consisting of euphorbiaceae, apocynaceae, asclepiadaceae, compositae, cactaceae and pinaceae families to a suitable particle size, (B) drying and preheating said particles in a reducing atmosphere under positive pressure (C) passing said particles through a thermal conversion zone containing a reducing atmosphere and with a residence time of 1 second to about 30 minutes at a temperature within the range of from about 200* C. To about 1000* C., (D) separately recovering the condensable vapors as liquids and the noncondensable gases in a condition suitable for use as chemical feedstocks or as hydrocarbon fuels.

  4. Seedling establishment of Ferocactus acanthodes in relation to drought

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, P.W.; Nobel, P.S.

    1981-08-01

    The 30 smallest seedlings of Ferocactus acanthodes (Lem.) Britton and Rose (Cactaceae) at a canyon site in the northwestern Sonoran desert averaged 2.5 cm in height. Based on field studies of CO/sub 2/ exchange throughout the year, the estimated time of their germination was late summer 1976. To explan why so little establishment occurred in recent years, the length of lethal drought for seedlings of various ages was calculated from the seedling geometry (volume-to-surface ratio), dehydration tolerance, and transpiration rates. Individual years were identified which had sufficiently long growing seasons relative to the length of the subsequent droughts to allow establishment. Eight of the last 18 y were found to be suitable for establishment of F. acanthodes, the latest year being 1976.

  5. Vegetation ecological restoration during geothermic exploratory perforation: A case study in Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Ortega-Rubio, A.; Salinas, F.; Naranjo, A. [and others

    1997-12-31

    At Las Tres Virgenes, B.C.S., Mexico developed the Geothermic exploratory drilling of the area. One of the main recommendations of our Environmental Impact Assessment Study includes transplantation of the plant individuals found in the zones of roads and drilling platforms. In this work we describe the methodologies used to transplant the vegetal individuals found in such zones. We listed the species selected and the survivorship rate obtained for every one of them. From a total of 4,266 transplanted individuals, including many endemic species, a total of 2349 survived. Members of the Agavaceae and Cactaceae families show the maximum survivorship rate, meanwhile the members of the Burseraceae, Euphorbiaceae and Fouqueriaceae families exhibited the minimum survivorship rate (between 12.7% and 20%).

  6. An efficient field and laboratory workflow for plant phylotranscriptomic projects1

    Science.gov (United States)

    Yang, Ya; Moore, Michael J.; Brockington, Samuel F.; Timoneda, Alfonso; Feng, Tao; Marx, Hannah E.; Walker, Joseph F.; Smith, Stephen A.

    2017-01-01

    Premise of the study: We describe a field and laboratory workflow developed for plant phylotranscriptomic projects that involves cryogenic tissue collection in the field, RNA extraction and quality control, and library preparation. We also make recommendations for sample curation. Methods and Results: A total of 216 frozen tissue samples of Caryophyllales and other angiosperm taxa were collected from the field or botanical gardens. RNA was extracted, stranded mRNA libraries were prepared, and libraries were sequenced on Illumina HiSeq platforms. These included difficult mucilaginous tissues such as those of Cactaceae and Droseraceae. Conclusions: Our workflow is not only cost effective (ca. $270 per sample, as of August 2016, from tissue to reads) and time efficient (less than 50 h for 10–12 samples including all laboratory work and sample curation), but also has proven robust for extraction of difficult samples such as tissues containing high levels of secondary compounds. PMID:28337391

  7. Nopal Cactus (Opuntia ficus-indica as a Source of Bioactive Compounds for Nutrition, Health and Disease

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    Karym El-Mostafa

    2014-09-01

    Full Text Available Opuntia ficus-indica, commonly referred to as prickly pear or nopal cactus, is a dicotyledonous angiosperm plant. It belongs to the Cactaceae family and is characterized by its remarkable adaptation to arid and semi-arid climates in tropical and subtropical regions of the globe. In the last decade, compelling evidence for the nutritional and health benefit potential of this cactus has been provided by academic scientists and private companies. Notably, its rich composition in polyphenols, vitamins, polyunsaturated fatty acids and amino acids has been highlighted through the use of a large panel of extraction methods. The identified natural cactus compounds and derivatives were shown to be endowed with biologically relevant activities including anti-inflammatory, antioxidant, hypoglycemic, antimicrobial and neuroprotective properties. The present review is aimed at stressing the major classes of cactus components and their medical interest through emphasis on some of their biological effects, particularly those having the most promising expected health benefit and therapeutic impacts.

  8. Ranking Tool Created for Medicinal Plants at Risk of Being Overharvested in the Wild

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    Lisa Marie Castle

    2014-05-01

    Full Text Available We developed an adaptable, transparent tool that can be used to quantify and compare vulnerability to overharvest for wild collected medicinal plants. Subsequently, we are creating a list of the most threatened medicinal plants in temperate North America. The new tool scores species according to their life history, the effects of harvest, their abundance and range, habitat, and demand. The resulting rankings, based on explicit criteria rather than expert opinion, will make it easier to discuss areas of vulnerability and set conservation priorities. Here we present scores for 40 species assessed using the At-Risk Tool and discuss the traits that led to different scores for six example species: echinacea (Echinacea angustifolia DC. Asteraceae, peyote (Lophophora williamsii (Lem. ex Salm-Dyck J.M. Coult. Cactaceae, sandalwood (Santalum spp. L. Santalaceae, stinging nettle (Urtica dioica L. Urticaceae, American ginseng (Panax quinquefolius L. Araliaceae and mayapple (Podophyllum peltatum L. Berberidaceae.

  9. Antiinflammatory flavonoids from Opuntia dillenii (Ker-Gawl) Haw. flowers growing in Egypt.

    Science.gov (United States)

    Ahmed, M S; El Tanbouly, N D; Islam, W T; Sleem, A A; El Senousy, A S

    2005-09-01

    Opuntia dillenii (Ker-Gawl) Haw. (Family Cactaceae), is used in folk medicine as an antidiabetic and antiinflammatory. The antiinflammatory activity of the alcohol extracts of the flowers, fruits and stems was carried out using the carrageenan-induced rat paw oedema model. The analgesic effect of the same extracts was evaluated using electric current as a noxious stimulus. The alcohol extract of the flowers revealed the most potent antiinflammatory effect and a pronounced analgesic action at a dose of 200 mg/kg. Bioassay-guided fractionation of this extract using VLC followed by Sephadex and paper chromatography, afforded three flavonoid glycosides, namely, kaempferol 3-O-alpha-arabinoside, isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside. Their identification was based on physical, chemical and spectroscopic data.

  10. Nopal cactus (Opuntia ficus-indica) as a source of bioactive compounds for nutrition, health and disease.

    Science.gov (United States)

    El-Mostafa, Karym; El Kharrassi, Youssef; Badreddine, Asmaa; Andreoletti, Pierre; Vamecq, Joseph; El Kebbaj, M'Hammed Saïd; Latruffe, Norbert; Lizard, Gérard; Nasser, Boubker; Cherkaoui-Malki, Mustapha

    2014-09-17

    Opuntia ficus-indica, commonly referred to as prickly pear or nopal cactus, is a dicotyledonous angiosperm plant. It belongs to the Cactaceae family and is characterized by its remarkable adaptation to arid and semi-arid climates in tropical and subtropical regions of the globe. In the last decade, compelling evidence for the nutritional and health benefit potential of this cactus has been provided by academic scientists and private companies. Notably, its rich composition in polyphenols, vitamins, polyunsaturated fatty acids and amino acids has been highlighted through the use of a large panel of extraction methods. The identified natural cactus compounds and derivatives were shown to be endowed with biologically relevant activities including anti-inflammatory, antioxidant, hypoglycemic, antimicrobial and neuroprotective properties. The present review is aimed at stressing the major classes of cactus components and their medical interest through emphasis on some of their biological effects, particularly those having the most promising expected health benefit and therapeutic impacts.

  11. Acute oral toxicity of Pereskia bleo and Pereskia grandifolia in mice

    Directory of Open Access Journals (Sweden)

    K S Sim

    2010-01-01

    Full Text Available Pereskia bleo and Pereskia grandifolia, belonging to the botanical family Cactaceae, have been traditionally used by the locals in Malaysia for treatment of various ailments. The current study reports the outcome of acute oral toxicity investigation of Pereskia bleo and Pereskia grandifolia, on ICR mice. No mortalities or evidence of adverse effects have been observed in ICR mice following acute oral administration at the highest dose of 2500 mg/ kg crude extracts of Pereskia bleo and Pereskia grandifolia. This is the first report on the acute oral toxicity of Pereskia bleo and Pereskia grandifolia and the findings of this study are in agreement with those of in vitro experiments and thus provide scientific validation on the use of the leaves of Pereskia bleo and Pereskia grandifolia.

  12. Usefulness of cpDNA markers for phylogenetic and phylogeographic analyses of closely related cactus species.

    Science.gov (United States)

    Bonatelli, I A S; Zappi, D C; Taylor, N P; Moraes, E M

    2013-02-28

    Although plastid DNA has been widely explored as a marker of choice for phylogeny and phylogeography studies, little is known about its utility for examining relationships between closely related species. The slow evolutionary rates inherent to chloroplast (cp) DNA make it difficult to perform lower level taxonomic analyses, particularly at the population level. We characterized the nucleotide variation and investigated the utility of eight noncoding cpDNA regions in four closely related species of the Pilosocereus aurisetus group (Cactaceae), an endemic taxon of eastern South America. The plastid intergenic spacers 5'-trnS-trnG, 3'-trnS-trnG and trnT-trnL were the most variable regions and were the most useful for lower level taxonomic comparisons, especially when used together. We conclude that an adequate combination of regions alongside indels as an additional character improves the usefulness of cpDNA for phylogenetic studies.

  13. Exploring the phytoremediation potential of cactus (Nopalea cochenillifera Salm. Dyck.) cell cultures for textile dye degradation.

    Science.gov (United States)

    Adki, Vinayak S; Jadhav, Jyoti P; Bapat, Vishwas A

    2012-07-01

    Cactaceae Nopalea cochenillifera cell cultures and intact plants (cladodes) transform various toxic textile dyes, including Red HE7B into less phytotoxic, non-hazardous metabolites. The [tentative] pathway analysis demonstrates that Red HE7B is transformed into 3-amino-5-imino-5,8-dihydronaphthalen-2-ol, 2-amino-6-(carboxycarbonyl)-3,4-dihydroxybenzoic acid, 4-aminophenol, and phenoL The significant induction of the activities of intracellular laccase (687%), tyrosinase (219%), azoreductase (144%), and 2,6-dichlorophenolindophenol reductase (167%) indicated the involvement of these enzymes in the transformation pathways of Red HE7B but these enzymes have not been definitively linked to the phytotransformation of this toxic dye. The present foundation work could add another plant candidate for phytoremediation of undesirable products from industry wastes and harmful chemicals.

  14. Genetic variability of an unusual apomictic triploid cactus--Haageocereus tenuis Ritter--from the Coast of Central Peru.

    Science.gov (United States)

    Arakaki, Mónica; Speranza, Pablo; Soltis, Pamela S; Soltis, Douglas E

    2013-01-01

    Haageocereus tenuis is a prostrate cactus restricted to a small area of 2 km(2) near the city of Lima, Peru. The species is triploid and propagates mainly through stem fragmentation. In addition, propagation via agamospermy is documented and adventitious embryony is also inferred as a mechanism. Although seedling recruitment has not been observed in nature, we have shown that asexually produced seeds are viable. About 45 adult individuals, plus 9 individuals obtained from seeds, were sampled and 5 microsatellite markers were used to assess genetic variability. Microsatellite analysis confirms that individuals from the only existing population are genetically identical and that the population likely represents a single clone. The absence of mutations in any individual, even in highly variable microsatellite loci, may indicate that the species is also of recent origin. Other prostrate species of Haageocereus are suspected to be occasional apomicts. This phenomenon has significant implications for the evolutionary biology and ecology of Haageocereus and other clonal Cactaceae.

  15. A multi-structural and multi-functional integrated fog collection system in cactus.

    Science.gov (United States)

    Ju, Jie; Bai, Hao; Zheng, Yongmei; Zhao, Tianyi; Fang, Ruochen; Jiang, Lei

    2012-01-01

    Multiple biological structures have demonstrated fog collection abilities, such as beetle backs with bumps and spider silks with periodic spindle-knots and joints. Many Cactaceae species live in arid environments and are extremely drought-tolerant. Here we report that one of the survival systems of the cactus Opuntia microdasys lies in its efficient fog collection system. This unique system is composed of well-distributed clusters of conical spines and trichomes on the cactus stem; each spine contains three integrated parts that have different roles in the fog collection process according to their surface structural features. The gradient of the Laplace pressure, the gradient of the surface-free energy and multi-function integration endow the cactus with an efficient fog collection system. Investigations of the structure-function relationship in this system may help us to design novel materials and devices to collect water from fog with high efficiencies.

  16. Protective role of cactus cladodes extract on sodium dichromate-induced testicular injury and oxidative stress in rats.

    Science.gov (United States)

    Hfaiedh, Mbarka; Brahmi, Dalel; Zourgui, Lazhar

    2014-06-01

    Cactus (Opuntia ficus-indica) is a xerophyte plant that belongs to the Cactaceae family. The present study was designed to investigate the possible protective effects of cactus cladodes extract (CCE) on sodium dichromate-induced testis damage in adult male Wistar rats. For this purpose, CCE at a dose of 100 mg/kg was orally administrated, followed by 10 mg/kg sodium dichromate (intraperitoneal injection). After 40 days of treatment, the rats were sacrificed, and the testes were excised for histological, lipid peroxidation (LPO), and antioxidant enzyme analyses. Sodium dichromate treatment significantly (PCactus cladodes supplementation minimized oxidative damage and reversed the impairment of spermatogenesis and testosterone production induced by sodium dichromate in the rat testis.

  17. A cactus-derived toxin-like cystine knot Peptide with selective antimicrobial activity.

    Science.gov (United States)

    Aboye, Teshome L; Strömstedt, Adam A; Gunasekera, Sunithi; Bruhn, Jan G; El-Seedi, Hesham; Rosengren, K Johan; Göransson, Ulf

    2015-05-04

    Naturally occurring cystine knot peptides show a wide range of biological activity, and as they have inherent stability they represent potential scaffolds for peptide-based drug design and biomolecular engineering. Here we report the discovery, sequencing, chemical synthesis, three-dimensional solution structure determination and bioactivity of the first cystine knot peptide from Cactaceae (cactus) family: Ep-AMP1 from Echinopsis pachanoi. The structure of Ep-AMP1 (35 amino acids) conforms to that of the inhibitor cystine knot (or knottin) family but represents a novel diverse sequence; its activity was more than 500 times higher against bacterial than against eukaryotic cells. Rapid bactericidal action and liposome leakage implicate membrane permeabilisation as the mechanism of action. Sequence homology places Ec-AMP1 in the plant C6-type of antimicrobial peptides, but the three dimensional structure is highly similar to that of a spider neurotoxin.

  18. Presence of arbuscular mycorrhizal fungi in South Florida native plants.

    Science.gov (United States)

    Fisher, Jack B; Jayachandran, K

    2005-11-01

    The roots of 27 species of South Florida plants in 15 families (including one cycad, six palms, one Smilax, and 19 dicotyledons) native to pine rockland and tropical hardwood hammock communities were examined for arbuscular mycorrhizal fungi (AMF). These plants grow in the biologically diverse but endangered Greater Everglades habitat. Roots from field-grown and potted plants were cleared and stained. All 27 species had AMF and include 14 species having an endangered or threatened status. The Paris-type colonization occurred in two species in the families Annonaceae and Smilacaceae. The Arum-type occurred in 22 species in the families Anacardiaceae, Arecaceae (Palmae), Boraginaceae, Cactaceae (questionable), Euphorbiaceae, Fabaceae, Lauraceae, Melastomataceae, Polygalaceae, Rubiaceae, Simaroubaceae, Ulmaceae, and Zamiaceae. Three species in the families Fabaceae, Lauraceae, and Simaroubaceae had a mix of Paris- and Arum-types. The results have implications for the restoration of these endangered plant communities in the Everglades.

  19. Propagação por sementes de Schlumbergera truncata (Haw. Moran (flor-de-maio em diferentes substratos - DOI: 10.4025/actascibiolsci.v29i3.480 Propagation by seeds of Schlumbergera truncata (Haw. Moran (florde- maio in different substrates - DOI: 10.4025/actascibiolsci.v29i3.480

    Directory of Open Access Journals (Sweden)

    Daniela Biondi

    2007-12-01

    Full Text Available Schlumbergera truncata (Haw. Moran (Cactaceae, conhecida como flor-demaio, é uma espécie herbácea epífita, nativa no Brasil, muito apreciada como planta ornamental pela beleza de suas flores. Esta espécie multiplica-se por sementes, mas comercialmente é propagada por estacas e enxertia. Este trabalho teve como objetivo avaliar a germinação de quatro variedades de cores desta espécie (rosa, vermelha, branca e amarela em cinco substratos (vermiculita de granulometria média, xaxim desfibrado, areia, substrato comercial Rendmax® Floreiras e mistura de 50% do substrato comercial Rendmax® Floreiras + 50% xaxim desfibrado. Os resultados mostraram que a espécie pode ser reproduzida com facilidade por sementes. O substrato comercial Rendmax® Floreiras proporcionou os melhores resultados.Schlumbergera truncata (Haw. Moran (Cactaceae, known as “flor-de-maio”, is a Brazilian endogenous herbaceous epiphytic species, much appreciated as ornamental for the beauty of its flowers. This species is multiplied by seeds, but commercially it is propagated by cuttings and grafting. The aim of this research was to evaluate the germination of four varieties of colors of this species (pink, red, white and yellow in five substrates (vermiculite, de-fibered tree ferns, sand, commercial substrate Rendmax® Floreiras and a mixture of 50% of the commercial substrate Rendmax® Floreiras + 50% tree ferns. The results showed that this species can be easily reproduced by seeds. The commercial substrate Rendmax® Floreiras provided the best germination result.

  20. Is geographical rarity frequent among the cacti of the Chihuahuan Desert? ¿Es la rareza geográfica frecuente entre las cactáceas del Desierto Chihuahuense?

    Directory of Open Access Journals (Sweden)

    Héctor M. Hernández

    2010-04-01

    Full Text Available With the aim of assessing the extent of geographical rarity of Mexican Cactaceae, we calculated the distribution size (area of occupancy of 142 species from the Chihuahuan Desert. In addition, using 2 variables (number of localities and range size, we preliminarily assessed their conservation status using the current IUCN Red List criteria. The results showed enormous variation in the areas of occupancy, although from the biogeographic and conservation perspective the most exceptional group comprises the extremely narrow endemics (42 species, whose range is restricted to areas smaller than 10 km2. Our results reinforce the reputation of this plant family as exceptionally rare geographically. We suggest that geographical rarity of Cactaceae in the Chihuahuan Desert is a natural phenomenon; however, we propose that the range of several species has been influenced by human activities. Regarding the conservation status of the species, 75 of them are categorized as Least concern. The remaining 67 species (47.2% fall in 1 of the 3 categories of threat (27 Vulnerable, 11 Endangered, and 29 Critically endangered. These figures confirm the critical conservation status of Mexican Cactaceae.Se calculó el tamaño de la distribución (área de ocupación de 142 especies de cactáceas del Desierto Chihuahuense, con el objeto de evaluar su grado de rareza geográfica. Además, mediante el uso de 2 variables (número de localidades y tamaño de distribución, se estimó de manera preliminar su estado de conservación usando los criterios actuales de la Lista Roja de la UICN. Los resultados mostraron gran variación en las áreas de ocupación. Sin embargo, desde una perspectiva biogeográfica y de la conservación, el grupo de especies más excepcional corresponde a las endémicas restringidas (42 spp., cuyas áreas de distribución son menores de 10 km². Los resultados fortalecen la reputación de las cactáceas de ser una familia de plantas excepcionalmente

  1. Aphididae (Hemiptera on ornamental plants in Córdoba (Argentina Aphididae (Hemiptera en plantas ornamentales de Córdoba (Argentina

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    Alfonsina Szpeiner

    2008-06-01

    Full Text Available In 30 surveys across Cordoba city and surroundings (Argentina I recorded, from November 2001 to May 2002, a total of 109 ornamental plants infested with aphid colonies. Surveys were conducted in the local botanical garden as well as homestead gardens and nurseries. Seventeen species of aphids were identified, 4 of which are new species in the area, from 47 ornamental plants. In a total of 59 plant/aphid associations, 38 new associations were found. I present the first record of aphids colonizing Cactaceae in Argentina. Most aphid colonies were medium and a high proportion (more than 40% showed alate individuals. The most frequent aphid species, Aphis gossypii Glover and Aphis craccivora Koch were especially associated with the most frequent damages: curled leaves or buds and necrosis, respectively. More than 30% of aphid colonies were attended by ants. Camponotus Mayr and Prenolepis Mayr were the ant genus attending the highest number of aphid species.En 30 censos realizados en la ciudad de Córdoba y alrededores (Argentina se registraron, entre noviembre de 2001 y mayo de 2002, un total de 109 plantas ornamentales infestadas de colonias de áfidos. Los censos fueron realizados en jardines, viveros y el jardín botánico de la ciudad. Se identificaron 47 especies de plantas ornamentales infestadas con 17 especies de áfidos (4 nuevas en el área. Se establecen 59 relaciones áfido/planta, de las cuales 38 son nuevas. Se obtuvo el primer registro de áfidos que colonizan Cactaceae en Argentina. La mayoría de las colonias de áfidos fueron medianas y una alta proporción (más del 40 % mostró individuos alados. Aphis gossypii Glover y Aphis craccivora Koch, fueron las especies más frecuentes y generaban los daños más conspicuos, como el enrollamiento y la necrosis foliar. Más del 30% de las colonias estuvieron atendidas por hormigas, siendo Camponotus Mayr y Prenolepis Mayr los géneros de hormigas que frecuentaban el mayor número de

  2. Análise morfométrica dos elementos traqueais em quatro espécies de Portulaca (Portulacaceae Morphometric analysis of tracheary elements in four Portulaca species

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    José Hernandes-Lopes

    2008-09-01

    Full Text Available Este trabalho aborda aspectos da caracterização morfológica dos elementos traqueais dos órgãos vegetativos de quatro espécies de Portulacaceae (Portulaca hirsutissima Camb., P. halimoides L., P. werdermannii Poelln., e de P. mucronata Link. ocorrentes na região Sudeste e Nordeste do Brasil. Os elementos de vaso observados são todos pequenos (This paper deals with the morphological features of the tracheary elements of the vegetative organs in four Portulaca species (Portulaca hirsutissima Camb., P. halimoides L., P. wedermannii Poelln. and P. mucronata Link. occurring in Southeast and Northeast Brazil. The vessel elements are small (< 25 μm and with simple perforation plate. The pattern of wall thickening varied from bordered pitting (in roots to scalariform and helicoidal (stem and leaves. Statistical methods show variation in vessel-element diameter in different vegetative organs; wider elements were observed in roots. Tracheids occurring in leaves of P. hirsutissima and P.wedermannii, have morphological features that are similar to terminal tracheids or tracheoid idiolasts frequently associated with xerophytes. The paedomorphic features (juvenlism observed here may be related, in part, to aspects of water transport and storage as described in Cactaceae.

  3. Pharmacognostical and phytochemical evaluation of Cactus grandiflorus (L. Britton and Rose

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    Rajat Rashmi

    2016-01-01

    Full Text Available Background: Cactus grandiflorus (L. Britton and Rose, Family: Cactaceae is an evergreen shrub with creeping aerial roots, used in Homoeopathy for atheromatous arteries, angina pectoris, and constriction of heart muscles, endocarditis, and heart weakness due to arteriosclerosis. Flowering stems are used in the preparation of medicine. Objective: The pharmacognostic and phytochemical studies are carried out to facilitate identification of correct species and standardized raw materials. Materials and Methods: Pharmacognostic studies of stem of authentic samples of Cactus grandiflorus (L. Britton and Rose have been carried out according to Trease and Evans, 1983, and Youngken 1959. To determine physicochemical constants, Indian Pharmacopoeia, 1970, was consulted and preliminary phytochemical properties were studied as per methods described by Trease and Evans, 1983. Results: Stem available in segments of variable length and thickness, roundish structure with 5 or 6 ridges and furrows with aerial roots, isodiametric cavities in cortex containing mucilage; aggregates of acicular and rhomboidal calcium oxalate crystals scattered in parenchymatous region are the key identification characteristic. Thin layer chromatography of chloroform extract of mother tincture reveals five spots with blue and violet colors. Conclusion: The macroscopic, microscopic, physicochemical, and phytochemical analysis of the authentic raw material were indicative to establish the standards for ensuring quality and purity of the drug.

  4. Wound healing properties and mucilage content of Pereskia aculeata from different substrates

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    Eber Goulart Carvalho

    2014-12-01

    Full Text Available Physiologic growth parameters Wound healing Pereskia aculeata Mill., Cactaceae, is a cactus with high mucilage production, well-known for its nutritional properties. Folk use consists on skin injuries, and mucilage is probably involved in the wound healing activity. This work studied some aspects of its cultivation, specifically regarding soil (substrate, to correlate the effects of nutritional content to mucilage production and to the wound-healing property. Plants were grown under five different soil treatment (sand, crude soil, sand and soil, sand and cattle manure, soil and cattle manure, and after eight months extracts were prepared by turbo-extraction to obtain a crude hydroethanolic extract. We evaluated the effects of these extracts on swelling index, cytotoxicity, and in vitro wound healing property. The results show that the substrate used in cultivation may interfere with mucilage production, but not with cytotoxicity and wound healing, this shows the safety of its use, despite the soil treatment received along the various biomes where P. aculeata is cultivated. Furthermore, morphological studies demonstrated the beneficial effect of the mucilage-containing extract on the fibroblast cell culture, corroborating its folk use for wound healing.

  5. Peyote identification on the basis of differences in morphology, mescaline content, and trnL/trnF sequence between Lophophora williamsii and L. diffusa.

    Science.gov (United States)

    Aragane, Masako; Sasaki, Yohei; Nakajima, Jun'ichi; Fukumori, Nobutaka; Yoshizawa, Masao; Suzuki, Yukiko; Kitagawa, Shigemi; Mori, Ken'ichiro; Ogino, Shuzo; Yasuda, Ichiro; Nagumo, Seiji

    2011-01-01

    Genus Lophophora (Cactaceae) has two species: Lophophora williamsii Coulter, which is called peyote, and L. diffusa Bravo. Although it was reported that L. williamsii contained mescaline and L. diffusa did not, we found L. williamsii specimens that did not contain mescaline. This finding indicated that the two species could not be differentiated in terms of mescaline content. Moreover, the relationship between mescaline content and morphology of the two species is also unknown. In this study, we attempted to clarify the difference in morphology, mescaline content, and DNA alignment of the chloroplast trnL/trnF region between L. williamsii and L. diffusa. As a result, L. williamsii specimens were classified into two groups. Group 1 had small protuberances on the epidermis, contained mescaline, and the analyzed region on the trnL/trnF sequence was 881 base pairs (bp) long in all except one (877 bp). Group 2 had large protuberances on the epidermis, did not contain mescaline, and the analyzed region was 893 bp long. On the other hand, L. diffusa had medium-sized protuberances on the epidermis, did not contain mescaline, and the analyzed region was 903 bp long. Also investigated was the potential application of the PCR-restriction fragment length polymorphism (RFLP) method as a means of identification based on the trnL/trnF sequence. By applying the PCR-RFLP method, the two species could be distinguished and L. williamsii specimens could be differentiated into group 1 and group 2.

  6. Vivipary in coastal cacti: a potential reproductive strategy in halophytic environments.

    Science.gov (United States)

    Cota-Sánchez, J Hugo; Reyes-Olivas, Alvaro; Sánchez-Soto, Bardo

    2007-09-01

    Vivipary, the germination of seeds within the fruit prior to abscission from the maternal plant, is an important event in plants. Two main types of vivipary are known in vascular plants: true vivipary and pseudovivipary. In crop grasses, pseudovivipary is an undesirable character as it results in lower yields. To date, vivipary in the Cactaceae has been reported for less than 20 species, most of which are cultivated. Here, we report viviparous (cryptoviviparous-a subcategory of true vivipary) cacti in nature in members of the tribes Cacteae and Pachycereeae (subfamily Cactoideae). We present four species inhabiting coastal plains in areas subject to periodic flooding, namely, Ferocactus herrerae, Stenocereus alamosensis, S. thurberi, and Pachycereus schottii. These species from localities in northwestern Mexico had viviparous fruits and offspring in different stages of development. A potential trend in the data indicates that the overall proportion of viviparous plants is higher in coastal flooding areas relative to halophytic, nonflooding areas. In our view, vivipary is a reproductive strategy that has evolved to provide a more efficient mechanism favoring germination and new avenues for survival by contributing to population maintenance and short-distance dispersal on halophytic substrates.

  7. Resolving a Prickly Situation: Involving Stakeholders in Invasive Cactus Management in South Africa

    Science.gov (United States)

    Novoa, Ana; Kaplan, Haylee; Wilson, John R. U.; Richardson, David M.

    2016-05-01

    The regulation and management of alien species can be contentious, particularly when the stakeholders who benefit from alien species are different from those who suffer the costs. We propose a consultative process involving relevant stakeholders in invasive species management decisions. The process involves (1) the identification of relevant stakeholders, (2) assessing their perceptions, (3) enhancing interaction between stakeholders, (4) assessing changes in stakeholders' perceptions following interactions with other stakeholders, and (5) developing management recommendations in collaboration with stakeholders. We demonstrate the application of the process using the family Cactaceae (`cacti') in South Africa. Many species of cacti have been introduced to the country over the past two centuries, mostly for horticulture, food and fodder, and hundreds of other species have been introduced in the past few decades (or are likely to be introduced soon) for horticulture. Using the proposed process enabled the negotiation and participation of all stakeholders in decision making and helped minimize contentious situations by clarifying stakeholder's beliefs and exploring consensus solutions. Consequently, management objectives were broadly supported by all stakeholders. These results will be included in a national cactus management strategy for South Africa.

  8. Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds

    Directory of Open Access Journals (Sweden)

    Carolina Carvalho Guilhon

    2015-01-01

    Full Text Available Pereskia bleo (Kunth DC. (Cactaceae is a plant commonly used in popular medicine in Malaysia. In this work, we evaluate the antinociceptive effect of P. bleo leaf extracts and isolated compounds in central antinociceptive model. Ethanol extract (E, hexane (H, ethyl acetate (EA, or butanol (B fractions (30, 50, or 100 mg/kg, p.o., sitosterol (from hexane and vitexin (from ethyl acetate, were administered to mice. Antinociceptive effect was evaluated in the hot plate and capsaicin- or glutamate-induced licking models. Morphine (1 mg/kg, p.o. was used as reference drug. Naloxone (1 mg/kg, i.p., atropine (1 mg/kg, i.p., and L-nitro arginine methyl ester (L-NAME, 3 mg/kg, i.p. were administered 30 min earlier (100 mg/kg, p.o. in order to evaluate the mechanism of the antinociceptive action. Higher dose of B developed an effect significantly superior to morphine-treated group. Naloxone prevented the antinociceptive effect of all fractions. L-NAME demonstrated effect against E, EA, and B. In all fractions, sitosterol and vitexin reduced the licking time after capsaicin injection. Glutamate-induced licking response was blocked by H, EA, and B. Our results indicate that Pereskia bleo fractions, sitosterol and vitexin, possessed a central antinociceptive effect. Part of this effect is mediated by opioid receptors and nitrergic pathway.

  9. The importance of pollinator generalization and abundance for the reproductive success of a generalist plant.

    Directory of Open Access Journals (Sweden)

    María Belén Maldonado

    Full Text Available Previous studies have examined separately how pollinator generalization and abundance influence plant reproductive success, but none so far has evaluated simultaneously the relative importance of these pollinator attributes. Here we evaluated the extent to which pollinator generalization and abundance influence plant reproductive success per visit and at the population level on a generalist plant, Opuntia sulphurea (Cactaceae. We used field experiments and path analysis to evaluate whether the per-visit effect is determined by the pollinator's degree of generalization, and whether the population level effect (pollinator impact is determined by the pollinator's degree of generalization and abundance. Based on the models we tested, we concluded that the per-visit effect of a pollinator on plant reproduction was not determined by the pollinators' degree of generalization, while the population-level impact of a pollinator on plant reproduction was mainly determined by the pollinators' degree of generalization. Thus, generalist pollinators have the greatest species impact on pollination and reproductive success of O. sulphurea. According to our analysis this greatest impact of generalist pollinators may be partly explained by pollinator abundance. However, as abundance does not suffice as an explanation of pollinator impact, we suggest that vagility, need for resource consumption, and energetic efficiency of generalist pollinators may also contribute to determine a pollinator's impact on plant reproduction.

  10. Ecological aspects of the casque-headed frog Aparasphenodon brunoi (Anura, Hylidae in a Restinga habitat in southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Daniel Oliveira Mesquita

    2004-09-01

    Full Text Available We describe some aspects of the ecology of Aparasphenodon brunoi, a species associated with bromeliads. We comment on the relationships of this species with bromeliad size, microhabitat use, diet and sexual dimorphism. This study was conducted on a Restinga habitat near Presidente Kennedy, state of Espírito Santo, southeastern coast of Brazil. When the animals were found inside the bromeliads, we measure bromeliad and head size of frogs. We analyzed stomach contents and determined the sex and reproductive condition. We found 17 individuals (58.6% in bromeliad leafs, six (20.7% in Cactaceae, three (10.3% in liana and three (10.3% on trunks. The correlation between head measurements and bromeliad size were high, indicating that animals apparently use bromeliads based on their size, which could be related to the minimization of water loss. The most common prey items were beetles, ants, and insect larvae, suggesting that the species is relatively generalist in prey consumption. Aparasphenodon brunoi showed significant sexual size and shape dimorphism with females having larger bodies than males (size and females having tibia, eye diameter and SVL larger than males (shape, but larger sample size and more detailed ecological and life history data are needed to elucidate the factors that have led to sexual size dimorphism.

  11. Comparison of Three Extraction Methods for Antimicrobial Substances from Opuntia%提取仙人掌抑菌物质三种方法的比较

    Institute of Scientific and Technical Information of China (English)

    韩淑琴; 杨洋

    2009-01-01

    目的 比较3种实验室提取仙人掌抑菌物质方法的优缺点.方法 以米邦塔食用仙人掌为原料,采用水浴浸提、超声波辅助提取、超临界提取3种方法提取其中的抑菌活性成分,并检测抑菌物质的抑菌性能.结果与结论 超临界提取法得到的活性物质抑菌效果最佳,超声波辅助提取次之,水浴提取法效果最差.%Objective To compare the three extraction methods for antimicrobial substances from opuntia. Methods With edible Cactus Opuntia milpa aha Haw (Cactaceae) as material, the antimicrobial substances were extracted by water bath, ultrasonic assistance, supercritical fluid extraction (SFE) method, respectively, and their antimicrobial activities were detected. Results and Conclusion The antimicrobial activity was highest for the antimicrobial substances extracted by SFE method, medium for that by ultrasonic assistance and lowest for that by water bath.

  12. Innovations from the “ivory tower”: Wilhelm Barthlott and the paradigm shift in surface science

    Science.gov (United States)

    2017-01-01

    This article is mainly about borders that have tremendous influence on our daily life, although many of them exist and act mostly unrecognized. In this article the first objective will be to address more generally the relation between university and society or industry, borders within universities, borders in thinking and the huge amount of misunderstandings and losses resulting from these obvious or hidden borders. In the second part and in more detail, the article will highlight the impact of the research conducted by Wilhelm Barthlott throughout his scientific career during which not only one border was removed, shifted or became more penetrable. Among the various fields of interest not mentioned here (e.g., systematics of Cactaceae, diversity and evolution of epiphytes, the unique natural history of isolated rocky outcrops called inselbergs, or the global distribution of biodiversity), plant surfaces and especially the tremendous diversity of minute structures on leaves, fruits, seeds and other parts of plants represent a common thread through 40 years of scientific career of Wilhelm Barthlott. Based on research that was regarded already old-fashioned in the 1970s and 1980s, systematic botany, results and knowledge were accumulated that, some 20 years later, initiated a fundamental turnover in how surfaces were recognized not only in biology, but even more evident in materials science. PMID:28326228

  13. 火龙果生物学特性、保健价值及其发展前景

    Institute of Scientific and Technical Information of China (English)

    郑伟; 王彬

    2004-01-01

    火龙果(Hylocereus undatus)为仙人掌科(Cactaceae)三角柱属(又称量天尺属,Hylocereus)的果用栽培品种。起源于中美洲,自然分布在哥斯达尼加、尼加拉瓜、墨西哥、古巴等国家和地区的热带雨林及沙漠地带,越南自然分布较广,人工栽培遍及中美洲、越南、泰国,我国台湾省及美国南部地区。火龙果集水果、花卉、蔬菜、保健、医药于一身,可称之为无价之宝,因此,已成为新奇、优良的绿色保健食品,倍受消费者青睐。火龙果的开发价值高,开发潜力大,市场前景广阔。

  14. Day-night variations in malate concentration, osmotic pressure, and hydrostatic pressure in Cereus validus

    Energy Technology Data Exchange (ETDEWEB)

    Luettge, U.; Nobel, P.S.

    1984-07-01

    Malate concentration and stem osmotic pressure concomitantly increase during nighttime CO/sub 2/ fixation and then decrease during the daytime in the obligate Crassulacean acid metabolism (CAM) plant, Cereus validus (Cactaceae). Changes in malate osmotic pressure calculated using the Van't Hoff relation match the changes in stem osmotic pressure, indicating that changes in malate level affected the water relations of the succulent stems. In contrast to stem osmotic pressure, stem water potential showed little day-night changes, suggesting that changes in cellular hydrostatic pressure occurred. This was corroborated by direct measurements of hydrostatic pressure using the Juelich pressure probe where a small oil-filled micropipette is inserted directly into chlorenchyma cells, which indicated a 4-fold increase in hydrostatic pressure from dusk to dawn. A transient increase of hydrostatic pressure at the beginning of the dark period was correlated with a short period of stomatal closing between afternoon and nighttime CO/sub 2/ fixation, suggesting that the rather complex hydrostatic pressure patterns could be explained by an interplay between the effects of transpiration and malate levels. A second CAM plant, Agave deserti, showed similar day-night changes in hydrostatic pressure in its succulent leaves. It is concluded that, in addition to the inverted stomatal rhythm, the oscillations of malate markedly affect osmotic pressures and hence water relations of CAM plants. 13 references, 4 figures.

  15. Composición florística de la cuenca del río Ilo-Moquegua y Lomas de Ilo, Moquegua, Perú

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    Mónica Arakaki

    2013-06-01

    Full Text Available Se presenta un estudio de la composición y riqueza de la flora vascular en la cuenca del Río Ilo- Moquegua y las Lomas de Ilo, a lo largo de una gradiente altitudinal que va desde los 0 hasta 4600 m, basado en colectas intensivas, revisión de ejemplares de herbario y consulta bibliográfica. De acuerdo al presente estudio, la flora vascular de la cuenca del río Ilo-Moquegua y las Lomas de Ilo está integrada por 63 familias, 233 géneros y 394 especies. Las Magnoliopsida representan el 83% de las especies y las Liliopsida el 15%. Las familias con mayor número de géneros y especies son Asteraceae (41 géneros y 60 especies, Poaceae (28 y 44, Solanaceae (11 y 32, Fabaceae (17 y 26, Malvaceae (11 y 21, Brassicaceae (10 y 15, Boraginaceae (9 y 15 y Cactaceae (10 y 14. Las formas biológicas dominantes son las hierbas (72%, seguidas por los arbustos (21%, plantas que pueden ser hierbas o arbustos (5%, árboles (2% y plantas parásitas (menos del 1%. De las 55 especies endémicas del Perú reportadas en el presente estudio, 10 están restringidas al departamento de Moquegua. Se presenta un total de 176 nuevas adiciones a la flora del departamento.

  16. Are cactus growth forms related to germination responses to light? A test using Echinopsis species

    Science.gov (United States)

    Ortega-Baes, Pablo; Aparicio-González, Mónica; Galíndez, Guadalupe; del Fueyo, Patricia; Sühring, Silvia; Rojas-Aréchiga, Mariana

    2010-05-01

    In this study, we investigated the effect of light regimen (white light vs. darkness) on the germination of 12 species of the Echinopsis genus (tribe Trichocereeae, Cactaceae). This genus presents a variety of growth forms and relatively small and uniform seed size. These traits allowed us to test, within the same linage and removing seed mass effect, the hypothesis that the germination response to light (indifferent to light or positive photoblastic) is related to growth form. Our results reject this hypothesis since no seeds germinated in darkness, so all of the species can be classified as being positively photoblastic. The proportion of seed germination with white light was significantly different among cactus growth forms. Columnar cacti (arborescent, creeping and short) showed a greater proportion of seed germination than barrel and globose cacti. The germination rate differed among growth forms and species. At constant temperatures, creeping columnar cacti presented a significantly higher germination rate than the other growth forms. With alternating temperatures, columnar cacti showed higher germination rates than the other growth forms. The low proportion of seeds that germinated for some species indicates that they show seed dormancy. Our results suggest that germination responses to light in the cactus family could be related to seed mass and phylogenetic constraints.

  17. Tandem mass spectrometry: analysis of complex mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Singleton, K.E.

    1985-01-01

    Applications of tandem mass spectrometry (MS/MS) for the analysis of complex mixtures results in increased specificity and selectivity by using a variety of reagent gases in both negative and positive ion modes. Natural isotopic abundance ratios were examined in both simple and complex mixtures using parent, daughter and neutral loss scans. MS/MS was also used to discover new compounds. Daughter scans were used to identify seven new alkaloids in a cactus species. Three of these alkaloids were novel compounds, and included the first simple, fully aromatic isoquinoline alkaloids reported in Cactaceae. MS/MS was used to characterize the chemical reaction products of coal in studies designed to probe its macromolecular structure. Negative ion chemical ionization was utilized to study reaction products resulting from the oxidation of coal. Possible structural units in the precursor coal were predicted based on the reaction products identified, aliphatic and aromatic acids and their anhydrides. The MS/MS method was also used to characterize reaction products resulting from coal liquefaction and/or extraction. These studies illustrate the types of problems for which MS/MS is useful. Emphasis has been placed on characterization of complex mixtures by selecting experimental parameters which enhance the information obtained. The value of using MS/MS in conjunction with other analytical techniques as well as the chemical pretreatment is demonstrated.

  18. Influência do substrato e do enraizamento na aclimatização de Melocactus glaucescens Buining & Brederoo propagados in vitro

    Directory of Open Access Journals (Sweden)

    Sheila Vitória Resende

    2010-12-01

    Full Text Available Melocactus glaucescens (Cactaceae é espécie endêmica da Bahia e está incluída na lista da IUCN e MMA como ameaçada de extinção. A transferência da condição in vitro para o ambiente ex vitro é uma etapa crítica, podendo ser um fator limitante para a produção das mudas micropropagadas. O objetivo deste trabalho foi analisar o efeito de diferentes substratos e do enraizamento na aclimatização de Melocactus glaucescens. As plantas propagadas in vitro foram mantidas sob 100% de luminosidade, com regas diárias por 75 dias. Os resultados demonstraram que o substrato adequado para a aclimatização deve conter 50% de terra vegetal e 50% de areia lavada; o tamanho mínimo do diâmetro e do comprimento da parte aérea para transferência para as condições ex vitro é de 5 mm e que as etapas de enraizamento in vitro e rustificação podem ser eliminadas da micropropagação de M. glaucescens. Estudos para demonstrar tempos de dessecação dos brotos acima de 5 mm são necessários, para se eliminar completamente a etapa do enraizamento in vitro para esta espécie.

  19. Effect of Opuntia humifusa Supplementation and Acute Exercise on Insulin Sensitivity and Associations with PPAR-γ and PGC-1α Protein Expression in Skeletal Muscle of Rats

    Directory of Open Access Journals (Sweden)

    Youngju Song

    2013-03-01

    Full Text Available This study examined whether Opuntia humifusa (O. humifusa, which is a member of the Cactaceae family, supplementation and acute swimming exercise affect insulin sensitivity and associations with PPAR-γ and PGC-1α protein expression in rats. Thirty-two rats were randomly divided into four groups (HS: high fat diet sedentary group, n = 8; HE: high fat diet acute exercise group, n = 8; OS: 5% O. humifusa supplemented high fat diet sedentary group, n = 8; OE: 5% O. humifusa supplemented high fat diet acute exercise group, n = 8. Rats in the HE and OE swam for 120 min. before being sacrificed. Our results indicated that serum glucose level, fasting insulin level and homeostasis model assessment of insulin resistance (HOMA-IR in OS were significantly lower compared to those of the HS (p < 0.01, p < 0.05, p < 0.05. In addition, PPAR-γ protein expression in the OS and OE was significantly higher than that of the HS and HE, respectively (p < 0.05, p < 0.01. PGC-1α and GLUT-4 protein expressions in the OS were significantly higher compared to those of the HS (p < 0.05, p < 0.05. From these results, O. humifusa supplementation might play an important role for improving insulin sensitivity through elevation of PPAR-γ, PGC-1α, and GLUT-4 protein expression in rat skeletal muscle.

  20. Two New Phenolic Carboxylic Acid Esters from Opuntia vulgaris%仙人掌中的两个新酚性羧酸酯

    Institute of Scientific and Technical Information of China (English)

    蒋建勤; 叶文才; 陈真; 楼凤昌; 闵知大

    2002-01-01

    从仙人掌块茎中分得二个新酚性羧酸酯和六个已知知化合物.根据其理化性质和波谱学方法鉴定它们的结构为2-(4-羟基-苄基)-苹果酸正丁酯 (1)、2-(4-羟基-苄基)-苹果酸-4-甲酯(2)、2-(羟基-苄基)-苹果酸(3)、蒲公英萜醇-3-乙酸酯(4)、软木三萜酮(5)、羽扇豆酮(6)、亚油酸甲酯(7)和油酸甲酯(8).斟中(1)和(2)为新化合物,(3)、(4)、(5)、(6)、(7)和(8)为首次从仙人掌中分得.%Two new phenolic carboxylic acid esters n-butyl eucomate(1) and methyl eucomate(2) and six known compounds eucomic acid(3), 3-β-acetyl-taraxerol (4), friedelin(5),lupenone(6),methyl linoleate(7) and methyl oleate(8) were isolated from the stems of Opuntia vulgaris Mill(Cactaceae). Their structures were detrmined on the basis of spectral meth ods. Compounds 3,4,6,7,8 were isolaled for the first time this plant.

  1. COMPOSIÇÃO FLORÍSTICA DE UM INSELBERGUE NO AGRESTE PARAIBANO, MUNICÍPIO DE ESPERANÇA, NORDESTE DO BRASIL

    Directory of Open Access Journals (Sweden)

    Paulinea Andreazza Ferreira Porto

    2008-01-01

    Full Text Available In order to determinate the floristic check-list of a inselberg at the Fazenda Timbaúba (35o52'50.3", 7o1'0.8", municipality of Esperança/PB, Agreste da Borborema Microregion, were realized the present study. The inselberg flora comprise 127 species, 101 genera and 53 families of wich the Leguminosae family with 17 species showed the higher richness, followed by the Asteraceae (9 spp., Poaceae (7 spp., Euphorbiaceae and Orchidaceae (6 spp. families. Bromeliaceae and Cactaceae, despite of not be the higher richness, are covering extents areas, suggesting a higher importance for those families in inselbergs colonization. Comparing the families with other inselberg floras, 29 of them also were raising in Quixadá/CE, 28 in Pão de Açúcar and 24 in Serra do Mar/RJ inselbergs. The majority of species showed xenomorphic adaptations due hydric stress observed in the Southern and Northeastern Brazilian inselbergs. The differences in the floristic composition, especially at genus and species level seem to result of different speciation pressure what aid the taxa with better adaptation at these environments.

  2. The influence of baboon predation and time in water on germination and early establishment of Opuntia stricta (Australian pest pear in the Kruger National Park

    Directory of Open Access Journals (Sweden)

    W.D. Lotter

    1999-01-01

    Full Text Available The alien invader weed, Opuntia stricta Haw (family Cactaceae, is seriously threatening biodiversity in veld habitats of the Kruger National Park. Basic biological and ecological information on the establishment, growth and reproduction of the species is necessary for the development of effective strategies for its control. The rapid spread of the plant is apparently mainly due to seed dispersal by baboon (Papio ursinus. Sixty percent of seed taken from baboon faeces resulted in seedlings that established. Although palatability criteria for ripe fruit were more favourable than for unripe or medium-ripe fruit, seed from fruit at all three degrees of ripeness germinated equally well, and seedling establishment was similar. Despite their lower acidity, as well as higher total soluble sugar content and pH, cladodes are not subject to herbivory to near the extent that ripe fruit are. Freshly collected seed kept in Sabie River water showed significantly better germination/emergence after seven days submersion (83 than at 14 or 28 days (52 and 66 , respectively. Results suggest that seed dispersal of the species by animals, principally baboon, is an important cause of rapidly expanding infestations, and that dissipation in water will intensify the problem. Current findings should contribute toward the development of long-term weed management strategies aimed at con- tainment/eradication of the weed.

  3. FLORA VASCULAR DE UMA ÁREA DE CAATINGA NO ESTADO DA PARAÍBA - NORDESTE DO BRASIL

    Directory of Open Access Journals (Sweden)

    ANTÔNIO CÁSSIO JUSTINO DOS SANTOS

    2010-01-01

    Full Text Available The caatinga biome covers around 70% of the North-Eastern territory and nevertheless the studies carried out about its natural riches are still insufficient especially tackling the floristics diversity of the biome as a whole, particularly in the state of Paraiba. For this reason the current study aimed to carry out the floristics survey of an area of caatinga in the municipality of Boqueirão, Paraiba State. In the area of study, it was registered 47 species distributed in 39 genera and 20 families of flowering plants. The families that stood out in number of species were: Fabaceae, with 12 species; Cactaceae, with five; Euphorbiaceae and Malvaceae, with four species each one and Convolvulaceae with three species, while the other families (15 showed one to two species each one. The woody component dominated over the shrubby, herbaceous, subshrubby and the woody vines components of the area studied. Based on the data obtained in this study, it was possible to correlate the taxa shown with the flora of different areas of caatinga, especially in the Northeast region, showing up the priority of carrying out floristics studies including other areas of the caatinga of Paraiba as well as of different states of the Brazilian Northeastern.

  4. Ampliando o conhecimento sobre a flora fanerogâmica do Ceará

    Directory of Open Access Journals (Sweden)

    Iracema Bezerra Loiola

    2014-10-01

    Full Text Available A pesquisa teve como objetivo geral conhecer, descrever e atualizar a distribuição geográfica dos representantes de Cactaceae, Capparaceae, Combretaceae e Erythroxylaceae ocorrentes no estado do Ceará. Para a coleta de amostras botânicas foram realizadas, no período de 2011 a 2013, quatro expedições de campo em 24 municípios pré-selecionados. Cada expedição teve duração de cinco dias e a participação de cinco componentes. As coleções foram depositadas no herbário EAC e duplicatas distribuídas a herbários nacionais. Os principais resultados obtidos foram: ampliação do número de coleções georreferenciadas e identificadas corretamente; produção de artigos sobre a flora cearense e descrição de uma nova espécie de Erythroxylum (Erythroxylaceae.

  5. Investigating the Potential of Using the Spatial and Spectral Information of Multispectral LiDAR for Object Classification

    Directory of Open Access Journals (Sweden)

    Wei Gong

    2015-09-01

    Full Text Available The abilities of multispectral LiDAR (MSL as a new high-potential active instrument for remote sensing have not been fully revealed. This study demonstrates the potential of using the spectral and spatial features derived from a novel MSL to discriminate surface objects. Data acquired with the MSL include distance information and the intensities of four wavelengths at 556, 670, 700, and 780 nm channels. A support vector machine was used to classify diverse objects in the experimental scene into seven types: wall, ceramic pots, Cactaceae, carton, plastic foam block, and healthy and dead leaves of E. aureum. Different features were used during classification to compare the performance of different detection systems. The spectral backscattered reflectance of one wavelength and distance represented the features from an equivalent single-wavelength LiDAR system; reflectance of the four wavelengths represented the features from an equivalent multispectral image with four bands. Results showed that the overall accuracy of using MSL data was as high as 88.7%, this value was 9.8%–39.2% higher than those obtained using a single-wavelength LiDAR, and 4.2% higher than for multispectral image.

  6. FLORÍSTICA E FITOSSOCIOLOGIA DE QUATRO REMANESCENTES VEGETACIONAIS EM ÁREAS DE SERRA NO CARIRI PARAIBANO

    Directory of Open Access Journals (Sweden)

    PEDRO THIAGO BARBOSA DE OLIVEIRA

    2009-01-01

    Full Text Available It was studied vegetal remainders into four mountain areas: Serra de Bodopitá (Area1, Serra de Bodocongó (Area 2, Serra do Monte (Area 3 and Serra do Canoió (Area 4 located in paraiban Cariri. The study aimed at determines the phytossociolical parameters and for this was used the program Mata Nativa 2. It was also calculated the indexes of Shannon, Pielou equability, diversity of species and families. From the 2972 sampled individuals, 633 belonged to Area 1, 602 to Area 2, 908 to Area 3 and 829 to Area 4. All of them were distributed in 54 species belonged to 25 botanic families. It was observed that the families Fabaceae and Euphorbiaceae had the largest number of species and individuals and after them Cactaceae, Anacardiaceae and Apocynaceae. The Shannon indexes values in Areas 1, 2, 3 and 4 were respectively 2.65; 2.93; 2.35 e 2.59 nats.esp.-1 . The founded densities were 3165 individuals.ha-1 in Area 1, 3010 individuals.ha-1 in Area 2, 4540 individuals.ha-1 in Area 3 and 4145 individuals.ha-1 in Area 4. The four areas had the same species with the most importance values being them: Caesalpinia pyramidalis, Aspidosperma pyrifolium, Bauhinia cheilantha, Piptadenia stipulacea.

  7. Plant population and habitat characteristics of the endemic Sonoran Desert cactus Peniocereus striatus in Organ Pipe Cactus National Monument, Arizona

    Science.gov (United States)

    Anderson, Greta; Rutman, Sue; Munson, Seth M.

    2010-01-01

    Peniocereus striatus (Brandegee) Buxb. (Cactaceae) is an endemic Sonoran Desert cactus that reaches its northern range limit in southwestern Arizona. One U.S. population occupies a small area of Organ Pipe Cactus National Monument near the U.S./Mexico international boundary, which has been monitored since 1939. An extensive survey conducted in 2002, covering 177 ha, resulted in the discovery of 88 new plants, in addition to the relocation of 57 plants found in previous surveys. Despite potential increases in population size and spatial distribution, mean plant height and number of basal stems has not significantly changed in recent years. Bud scars revealed that a majority of the population was sexually mature. Peniocereus striatus occurrence increased with decreasing slope, spanned every slope aspect, and was highest on rocky soils, but was noticeably low on west and northwest slopes and areas where severe land degradation had previously occurred. Over half of P. striatus plants were nursed by shrubs and subshrubs, while 40% occurred under leguminous trees. A severe frost in January 2002 top-killed 19% of the population, with the greatest damage in drainage bottoms. However, long-term (1944–2002) climate records show that there has been an overall increase in the number of frost free days in the region, which, coupled with land use change, has implications for the future health of this population.

  8. Aphididae (Hemiptera on ornamental plants in Córdoba (Argentina

    Directory of Open Access Journals (Sweden)

    Alfonsina SZPEINER

    2008-01-01

    Full Text Available En 30 censos realizados en la ciudad de Córdoba y alrededores (Argentina se registraron, entre noviembre de 2001 y mayo de 2002, un total de 109 plantas ornamentales infestadas de colonias de áfidos. Los censos fueron realizados en jardines, viveros y el jardín botánico de la ciudad. Se identificaron 47 especies de plantas ornamentales infestadas con 17 especies de áfidos (4 nuevas en el área. Se establecen 59 relaciones áfido/planta, de las cuales 38 son nuevas. Se obtuvo el primer registro de áfidos que colonizan Cactaceae en Argentina. La mayoría de las colonias de áfidos fueron medianas y una alta proporción (más del 40 % mostró individuos alados. Aphis gossypii Glover y Aphis craccivora Koch, fueron las especies más frecuentes y generaban los daños más conspicuos, como el enrollamiento y la necrosis foliar. Más del 30% de las colonias estuvieron atendidas por hormigas, siendo Camponotus Mayr y Prenolepis Mayr los géneros de hormigas que frecuentaban el mayor número de especies de áfidos.

  9. Immunoprotective activity and antioxidant properties of cactus (Opuntia ficus indica) extract against chlorpyrifos toxicity in rats.

    Science.gov (United States)

    Smida, Amani; Ncibi, Saida; Taleb, Jihen; Ben Saad, Anouar; Ncib, Sana; Zourgui, Lazhar

    2017-02-03

    Opuntia ficus indica (family Cactaceae) is a typical Mediterranean plant, mainly used in food and traditional folk medicine. The present study was designed to evaluate the protective effect of Opuntia ficus indica extract against chlorpyrifos (CPF)-induced immunotoxicity in rats. The experimental animals consisted of four groups of Wistar rats (5-6 weeks old) of eight each: a control group, a group treated with CPF (10mg/kg), a group treated with Opuntia ficus indica extract (100mg/kg), and a group treated with cactus extract then treated with CPF. These components were daily administered by gavage for 30days. After treatment, immunotoxicity was estimated by a count of thymocytes, splenocytes, stem cells in the bone marrow, relative weights of thymus and spleen, DNA aspects, and oxidative stress status in these organs. Results showed that CPF could induce thymus atrophy, splenomegaly, and a decrease in the cell number in the bone marrow. It also increased the oxidative stress markers resulting in elevated levels of the lipid peroxidation with a concomitant decrease in the levels of enzymatic antioxidants (SOD, CAT, GPx) in both spleen and thymus, and also degradation of thymocyte and splenocyte DNA. Consistent histological changes were found in the spleen and thymus under CPF treatment. However, administration of Opuntia ficus indica extract was found to alleviate this CPF-induced damage.

  10. The n-butanolic extract of Opuntia ficus-indica var. saboten enhances long-term memory in the passive avoidance task in mice.

    Science.gov (United States)

    Kim, Jong Min; Kim, Dong Hyun; Park, Se Jin; Park, Dong Hyun; Jung, Seo Yun; Kim, Hyoung Ja; Lee, Yong Sup; Jin, Changbae; Ryu, Jong Hoon

    2010-08-16

    Opuntia ficus-indica var. saboten Makino (Cactaceae) is used to treat burns, edema, dyspepsia, and asthma in traditional medicine. The present study investigated the beneficial effects of the n-butanolic extract of O. ficus-indica var. saboten (BOF) on memory performance in mice and attempts to uncover the mechanisms underlying its action. Memory performance was assessed with the passive avoidance task, and western blotting and immunohistochemistry were used to measure changes in protein expression and cell survival. After the oral administration of BOF for 7 days, the latency time in the passive avoidance task was significantly increased relative to vehicle-treated controls (P<0.05). Western blotting revealed that the expression levels of brain-derived neurotrophic factor (BDNF), phosphorylated cAMP response element binding-protein (pCREB), and phosphorylated extracellular signal-regulated kinase (pERK) 1/2 were significantly increased in hippocampal tissue after 7 days of BOF administration (P<0.05). Doublecortin and 5-bromo-2-deoxyuridine immunostaining also revealed that BOF significantly enhanced the survival of immature neurons, but did not affect neuronal cell proliferation in the subgranular zone of the hippocampal dentate gyrus. These results suggest that the subchronic administration of BOF enhances long-term memory, and that this effect is partially mediated by ERK-CREB-BDNF signaling and the survival of immature neurons.

  11. Análisis del comportamiento de mercado de la pitahaya (Hylocereus undatus en Costa Rica

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    Olman Quirós Madrigal

    2010-10-01

    Full Text Available La pitahaya (Hylocereus undatus es una planta que pertenece a la familia de las cactaceae, que se puede consumir como fruta fresca o procesada como pulpa; sin embargo, en Costa Rica su uso no está muy difundido, por lo que el mercado meta es la población de origen nicaragüense residente en el país, quienes catalogan el producto como de consumo tradicional o masivo.Es por esto que resulta importante retomar el estudio de los componentes del mercado, en donde se destaca no sólo por ser una fruta con componentes nutricionales que aportan beneficios a la salud humana, sino tambien porque suple una demanda latente y su cultivo puede ser una actividad alternativa para productores de zonas con condiciones agroecológicas adversas. En relación con el abastecimiento del producto en el mercado nacional, se enfatiza que su procedencia es nacional pero principalmente se importa como fruta fresca y pulpa de Nicaragua y, en menor grado, de Colombia.El precio de la pitahaya en el mercado nacional está estrechamente relacionado con los precios de importación, ya que la oferta principal proviene del producto importado, por lo que ha tenido un comportamiento creciente al pasar, de US$0,574 en el año 2003, a US$1,18 por kg en el 2007.

  12. Innovations from the "ivory tower": Wilhelm Barthlott and the paradigm shift in surface science.

    Science.gov (United States)

    Neinhuis, Christoph

    2017-01-01

    This article is mainly about borders that have tremendous influence on our daily life, although many of them exist and act mostly unrecognized. In this article the first objective will be to address more generally the relation between university and society or industry, borders within universities, borders in thinking and the huge amount of misunderstandings and losses resulting from these obvious or hidden borders. In the second part and in more detail, the article will highlight the impact of the research conducted by Wilhelm Barthlott throughout his scientific career during which not only one border was removed, shifted or became more penetrable. Among the various fields of interest not mentioned here (e.g., systematics of Cactaceae, diversity and evolution of epiphytes, the unique natural history of isolated rocky outcrops called inselbergs, or the global distribution of biodiversity), plant surfaces and especially the tremendous diversity of minute structures on leaves, fruits, seeds and other parts of plants represent a common thread through 40 years of scientific career of Wilhelm Barthlott. Based on research that was regarded already old-fashioned in the 1970s and 1980s, systematic botany, results and knowledge were accumulated that, some 20 years later, initiated a fundamental turnover in how surfaces were recognized not only in biology, but even more evident in materials science.

  13. Resolving a Prickly Situation: Involving Stakeholders in Invasive Cactus Management in South Africa.

    Science.gov (United States)

    Novoa, Ana; Kaplan, Haylee; Wilson, John R U; Richardson, David M

    2016-05-01

    The regulation and management of alien species can be contentious, particularly when the stakeholders who benefit from alien species are different from those who suffer the costs. We propose a consultative process involving relevant stakeholders in invasive species management decisions. The process involves (1) the identification of relevant stakeholders, (2) assessing their perceptions, (3) enhancing interaction between stakeholders, (4) assessing changes in stakeholders' perceptions following interactions with other stakeholders, and (5) developing management recommendations in collaboration with stakeholders. We demonstrate the application of the process using the family Cactaceae ('cacti') in South Africa. Many species of cacti have been introduced to the country over the past two centuries, mostly for horticulture, food and fodder, and hundreds of other species have been introduced in the past few decades (or are likely to be introduced soon) for horticulture. Using the proposed process enabled the negotiation and participation of all stakeholders in decision making and helped minimize contentious situations by clarifying stakeholder's beliefs and exploring consensus solutions. Consequently, management objectives were broadly supported by all stakeholders. These results will be included in a national cactus management strategy for South Africa.

  14. ENRAIZAMENTO DE ESTACAS DE PITAYA VERMELHA EM DIFERENTES SUBSTRATOS

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    Marco Túlio Habib Silva

    2006-01-01

    Full Text Available The red pitaya is a cactacea that has been more and more demanded both for theproducers and for the consumers. Nevertheless, there are still several aspects about its cultivation that need to be elucidated, in order to provide a good culture development and profitability to the producer. This work was done aiming to study the effect of substrate in the vegetative propagation of this fruit, commonly done through cutting. The experiment was carried out in a lath house from the Departamento de Produção Vegetal of FCAV - UNESP. Cuttings of adult plant were used and placed in different substrates for rooting, composing the following treatments: (1 soil, sand and manure of tanned corral mixture, in the proportion of 3:1:1; (2 coconut fiber (Amafibra; (3 sand and (4 commercial substrate (Plantmax. For each treatment, 4 replications with 10 cuttings were done. Fresh and dry mass of roots (grams and volume (mL were evaluated. A completely randomized design was used, and data evaluated by Tukey test at 5% probability. In the conditions in which the experiment was accomplished, one can conclude that there was influence of the substrate in the fresh mass and volume of the roots, being the substrate (1 the most appropriate for seedlings formation of this species.

  15. Structural investigations on betacyanin pigments by LC NMR and 2D NMR spectroscopy.

    Science.gov (United States)

    Stintzing, Florian C; Conrad, Jürgen; Klaiber, Iris; Beifuss, Uwe; Carle, Reinhold

    2004-02-01

    Four betacyanin pigments were analysed by LC NMR and subjected to extensive NMR characterisation after isolation. Previously, low pH values were applied for NMR investigations of betalains resulting in rapid degradation of the purified substances thus preventing extensive NMR studies. Consequently, up to now only one single (13)C NMR spectrum of a betalain pigment, namely that of neobetanin (=14,15-dehydrobetanin), was available. Because of its sufficient stability under highly acidic conditions otherwise detrimental for betacyanins, this pigment remained an exemption. Since betalains are most stable in the pH range of 5-7, a new solvent system has been developed allowing improved data acquisition through improved pigment stability at near neutral pH. Thus, not only (1)H, but for the first time also partial (13)C data of betanin, isobetanin, phyllocactin and hylocerenin isolated from red-purple pitaya [Hylocereus polyrhizus (Weber) Britton & Rose, Cactaceae] could be indirectly obtained by gHSQC- and gHMQC-NMR experiments.

  16. MORPHOPHYSIOLOGICAL ASPECTS OF SELENICEREUS MEGALANTHUS (K. SCHUM EX VAUPEL MORAN

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    M. Sorace

    2016-07-01

    Full Text Available Selenicereus megalanthus (K. Schumer Vaupel Moran is known as yellow Pitaya because of yellow peel color. Originated from Colombia, Peru, Ecuador and Bolivia, it belongs to the family Cactaceae and has climbing habit, besides being edible and currently grown. In Brazil the production of yellow pitaya is incipient. Pitaya propagates through cuttings, seed or grafting. Its seeds have sarcotesta mucilaginous, which may be a deterrent factor or decrease germination. This study aimed to study biometric aspects and germination of seeds with and without mucilage removal. The removal of mucilage was made by immersion in 25% sucrose solution and were evaluated biometric aspects of fruit and seed quality through tests of germination and tetrazolium, rate of germination speed and imbibition curve. Through biometrics establishes the relationship between the size of the fruit and seed number, where the number of seeds per unit mass is greater in smaller fruits. The continuous production of mucilage prevented the establishment of imbibition curve. The result obtained in the tetrazolium test was not consistent with the germination. Seeds with mucilage removal by pretreatment with sucrose solution showed better germination and IVG, producing stronger plants.

  17. Carotenoids profile and total polyphenols in fruits of Pereskia aculeata Miller

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    Tânia da Silveira Agostini-Costa

    2012-03-01

    Full Text Available Pereskia aculeata Mill. (Ora-pro-nóbis is a native cactaceae from tropical America, whose leaves have high protein content. In Brazil it is found in all territorial extension between the states of Bahia and Rio Grande do Sul. Most studies have focused on chemical characterization of the leaves of this specie. The objective was to assess the carotenoids profile and the total polyphenols present in the fruits of P. aculeate. Carotenoids were determined by HPLC-PAD (high performance liquid chromatography - photodiode array detector, total polyphenols were determined by Folin-Ciocalteu and vanillin methods. Trans-β-carotene was the main carotenoid, followed by α-carotene, lutein and other minor carotenoids. It was found 64.9 ± 1.1 mg.100g-1 of gallic acid equivalent, 14.8 ± 0.2 mg.100g-1 of catechin equivalent. Carotenoid identification of P. aculeate fruits are presented here by the first time and indicate that these fruits can be researched as source of bioactive substances, especially antioxidant and provitamin A carotenoids.

  18. Two new species of Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoids of Hypogeococcus spp. (Hemiptera: Pseudococcidae), with taxonomic notes on some congeneric taxa.

    Science.gov (United States)

    Triapitsyn, Serguei V; Logarzo, Guillermo A; Aguirre, María B; Aquino, Daniel A

    2014-09-15

    Two new species of Anagyrus Howard (Hymenoptera: Encyrtidae) are described from Argentina, A. cachamai Triapitsyn, Logarzo & Aguirre sp. n. (Catamarca, Córdoba, Salta and Tucumán Provinces) and A. quilmes Triapitsyn, Logarzo & Aguirre sp. n. (Catamarca, Salta and Tucumán). Both new species are parasitoids of Hypogeococcus spp. (Hemiptera: Pseudococcidae). Anagyrus cachamai is a parasitoid of H. pungens Granara de Willink on Alternanthera paronychioides, A. pungens and Gomphrena sp. (Amaranthaceae), and also of a Hypogeococcus sp. on Cleistocactus baumannii and Hypogeococcus sp. on C. smaragdiflorus (Cactaceae). Anagyrus quilmes is a parasitoid of H. pungens on A. paronychioides, A. pungens and Gomphrena sp. Other biological traits of the new species are also reported. These parasitoids may be of importance as potential candidate biological control agents against a Hypogeococcus sp., commonly called the Harrisia cactus mealybug and identified as H. pungens, but possibly not belonging to that species. This mealybug threatens the native cacti in some Caribbean islands and Florida, USA, and is devastating the native columnar cacti in Puerto Rico. Illustrations and taxonomic notes on the type specimens of some other, little known described species of Anagyrus from Argentina and Chile are provided, and a key to females of the 14 species of Anagyrus known from Argentina is given. Anagyrus nigriceps (De Santis) syn. n. is synonymized under A. bellator (De Santis). Lectotypes are designated for Paranusia bifasciata Brèthes, Philoponectroma pectinatum Brèthes, and Protanagyrus aciculatus Blanchard. 

  19. Reversing gastric mucosal alterations during ethanol-induced chronic gastritis in rats by oral administration of Opuntia ficus- indica mucilage

    Institute of Scientific and Technical Information of China (English)

    Ricardo Vázquez-Ramírez; Marisela Olguín-Martínez; Carlos Kubli-Garfias; Rolando Hernández-Mu(n)oz

    2006-01-01

    AIM: To study the effect of mucilage obtained from cladodes of Opuntia ficus-indica (Cactaceae) on the healing of ethanol-induced gastritis in rats.METHODS: Chronic gastric mucosa injury was treated with mucilage (5 mg/kg per day) after it was induced by ethanol. Lipid composition, activity of 5'-nucleotidase (a membrane-associated ectoenzyme) and cytosolic activities of lactate and alcohol dehydrogenases in the plasma membrane of gastric mucosa were determined.Histological studies of gastric samples from the experimental groups were included.RESULTS: Ethanol elicited the histological profile of gastritis characterized by loss of the surface epithelium and infiltration of polymorphonuclear leukocytes.Phosphatidylcholine (PC) decreased and cholesterol content increased in plasma membranes of the gastric mucosa. In addition, cytosolic activity increased while the activity of alcohol dehydrogenases decreased. The administration of mucilage promptly corrected these enzymatic changes. In fact, mucilage readily accelerated restoration of the ethanol-induced histological alterations and the disturbances in plasma membranes of gastric mucosa, showing a univocal anti-inflammatory effect.The activity of 5'-nucleotidase correlated with the changes in lipid composition and the fluidity of gastric mucosal plasma membranes.CONCLUSION: The beneficial action of mucilage seems correlated with stabilization of plasma membranes of damaged gastric mucosa. Molecular interactions between mucilage monosaccharides and membrane phospholipids,mainly PC and phosphatidylethanolamine (PE), may be the relevant features responsible for changing activities of membrane-attached proteins during the healing process after chronic gastric mucosal damage.

  20. Phyllotactic pattern formation in early stages of cactus ontogeny

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    Edyta M. Gola

    2011-01-01

    Full Text Available Representatives of the family Cactaceae are characterized by a wide range of phyllotaxis. To assess the origin of this diversity, early stages of phyllotactic pattern formation were examined in seedlings. The analysis of the sequence of areole initiation revealed intertribal differences. In seedlings from the Trichocereeae (Gymnocalycium, Rebutia and Notocacteae (Parodia tribes, two opposite cotyledonal areoles developed as the first elements of a pattern. Usually, next pair of areoles was initiated perpendicularly to cotyledonal areoles, starting the decussate pattern. This pattern was subsequently transformed into bijugate or into simple spiral phyllotaxis. In seedlings from the Cacteae tribe (Mammillaria and Thelocactus, cotyledonal areoles were never observed and the first areoles always appeared in the space between cotyledons. It was either areole pair (mainly in Mammillaria, starting a decussate pattern, or a single areole (mainly in Thelocactus quickly followed by areoles spirally arranged, usually in accordance with the main Fibonacci phyllotaxis. Differences in the initial stages of pattern formation do not fully explain the phyllotaxis diversity in mature cacti. Only two, the most common phyllotactic patterns occurred in the early development of studied seedlings, i.e. the main Fibonacci and the decussate pattern. Discrepancy in the range of phyllotactic spectra in seedlings and in mature plants suggests that phyllotaxis diversity emerges during further plant growth. Initial phyllotactic transformations, occurring already in the very early stages, indicate great plasticity of cactus growth and seem to support the hypothesis of the ontogenetic increase of phyllotaxis diversity due to transformations.

  1. Thermoluminescence characterization of the irradiated minerals extracted from nopal

    Science.gov (United States)

    Cruz-Zaragoza, E.; Furetta, C.; Kitis, G.; Gomez, B. T.; Polymeris, G. S.; Tsirliganis, N.; Loukou, Z.

    2006-05-01

    The mineral fraction from dehydrated nopal leaves (Opuntia ficus indica ) belonging to the Cactaceae family was extracted and selected by sizes of 10 and 74 mu m and exposed to 60 Co gamma radiation at different doses in the range 70 Gy-20 kGy. The glow-curves from these polyminerals show a thermoluminescence (TL) band with one very intense peak at a temperature around 150 degrees C and a second one emerging in the high temperature region, moving in a large zone of temperature values according to the preparation used and the level of irradiation. The XRD analysis shows a composition of both biominerals as whewellite and weddellite and a mineral fraction as anorthoclase and quartz. The main TL characteristics of the polymineral content of the nopal was analyzed, i.e . the TL response at different doses and fading during storage at room temperature. The activation energy of the traps responsible for the TL emission was also investigated and a possible continuous distribution of traps is discussed. A review of the scientific literature shows that this is the first time that a TL study on nopal ionized by irradiation has been carried out.

  2. COMUNIDADE EPIFÍTICA DE SYAGRUS CORONATA (MART. BECC. (ARECACEAE EM ÁREAS DE PASTAGENS NA CAATINGA, BAHIA

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    ULDÉRICO RIOS OLIVEIRA

    2015-01-01

    Full Text Available The aim of this study was to provide information on the ecology and floristic composition of vascular epiphytes on specimens of licurizeiro (Syagrus coronata, palm tree native to the Brazilian Backlands, whose individuals were isolated in pasture areas in the municipality of Várzea da Roça - BA. The epiphytic species were classified according to their ecological category and dispersion forms. The vascular epiphytes were represented by 26 species, 21 genera and seven families, and Bromeliaceae (8 spp., Cactaceae (7 spp., Polypodiaceae (4 spp. and Orchidaceae (3 spp. the most representative. The true holoepiphytes and accidental epiphytes were the most frequent categories, gathering, respectively, 42.31% and 30.76% of the species inven-toried. Regarding the dispersion forms, it is observed the predominant of zoochoric species (14 spp. while anemochoric were represented by 12 species. The results show the importance of licurizeiro in the maintenance of the epiphytic community in areas of Caatinga and show its role as maintainer of biological diversity in an-thropogenic environments.

  3. How prevalent is crassulacean acid metabolism among vascular epiphytes?

    Science.gov (United States)

    Zotz, Gerhard

    2004-01-01

    The occurrence of crassulacean acid metabolism (CAM) in the epiphyte community of a lowland forest of the Atlantic slope of Panama was investigated. I hypothesized that CAM is mostly found in orchids, of which many species are relatively small and/or rare. Thus, the relative proportion of species with CAM should not be a good indicator for the prevalence of this photosynthetic pathway in a community when expressed on an individual or a biomass basis. In 0.4 ha of forest, 103 species of vascular epiphytes with 13,099 individuals were found. As judged from the C isotope ratios and the absence of Kranz anatomy, CAM was detected in 20 species (19.4% of the total), which were members of the families Orchidaceae, Bromeliaceae, and Cactaceae. As predicted, the contribution of CAM epiphytes to the total number of individuals and to total biomass (69.6 kg ha(-1)) was considerably lower (3.6% or 466 individuals and, respectively, 3.0% or 2.1 kg ha(-1)).

  4. JENIS - JENIS TUMBUHAN YANG DIGUNAKAN SEBAGAI BAHAN PERAWATAN KECANTIKAN DI PURI DAMAI DESA SINGAKERTA, KECAMATAN UBUD, KABUPATEN GIANYAR

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    Anak Agung Istri Ratih Gayatri

    2015-05-01

    Full Text Available Indonesia has many variaties of plant which give a benefit for house, clath, meals,medicine even for beauty treatment. A beauty treatment made from chemical agent causing anegative effect, so searching for an alternative agent for beauty treatment without giving anegative side effect was needed. The goal of has research is to know find species of plants,methods of process also how to process the plants become a herbal beauty treatment agent atPuri Damai which located in Singakerta village, district of ubud, Gianyar region. They takedirect observation, description and identification methods. According to the result of theresearch were found 25 species, 19 suku of plants 3 euphorbiaceae, 2 asteraceae, 2araliaceae, 2 zingiberaceae, pandanaceae, asphodelaceae, poaceae, fabaceae, anonaceae,magnoliaceae, arecaceae, laminaceae, malvaceae, cactaceae, oleaceae, apocynaceae,agavaceae, melastomataceae, rutaceae. Hair vertilizer oil mostly was made from the leaf ofthe plant takes around 56% and for massage oil take the flowers around 53 %. The methodswas taken to produce hair vertilizer oil and ingredients was fried in 1 liter frying oil between6 – 8 hours long in little fire. Direction of use for hair vertilizer oil is rubbing the oil on thecalp and hair before is after washing the hair. The use of massage oil the oli to the body andgive a massage.Keywords : Etnobotany, Beauty treatment, Observation.

  5. Reversing gastric mucosal alterations during ethanol-induced chronic gastritis in rats by oral administration of Opuntia ficus-indica mucilage

    Science.gov (United States)

    Vázquez-Ramírez, Ricardo; Olguín-Martínez, Marisela; Kubli-Garfias, Carlos; Hernández-Muñoz, Rolando

    2006-01-01

    AIM: To study the effect of mucilage obtained from cladodes of Opuntia ficus-indica (Cactaceae) on the healing of ethanol-induced gastritis in rats. METHODS: Chronic gastric mucosa injury was treated with mucilage (5 mg/kg per day) after it was induced by ethanol. Lipid composition, activity of 5’-nucleotidase (a membrane-associated ectoenzyme) and cytosolic activities of lactate and alcohol dehydrogenases in the plasma membrane of gastric mucosa were determined. Histological studies of gastric samples from the experimental groups were included. RESULTS: Ethanol elicited the histological profile of gastritis characterized by loss of the surface epithelium and infiltration of polymorphonuclear leukocytes. Phosphatidylcholine (PC) decreased and cholesterol content increased in plasma membranes of the gastric mucosa. In addition, cytosolic activity increased while the activity of alcohol dehydrogenases decreased. The administration of mucilage promptly corrected these enzymatic changes. In fact, mucilage readily accelerated restoration of the ethanol-induced histological alterations and the disturbances in plasma membranes of gastric mucosa, showing a univocal anti-inflammatory effect. The activity of 5’-nucleotidase correlated with the changes in lipid composition and the fluidity of gastric mucosal plasma membranes. CONCLUSION: The beneficial action of mucilage seems correlated with stabilization of plasma membranes of damaged gastric mucosa. Molecular interactions between mucilage monosaccharides and membrane phospholipids, mainly PC and phosphatidylethanolamine (PE), may be the relevant features responsible for changing activities of membrane-attached proteins during the healing process after chronic gastric mucosal damage. PMID:16865772

  6. Genetic structure is determined by stochastic factors in a natural population of Drosophila buzzatii in Argentina.

    Science.gov (United States)

    Vilardi, J C; Hasson, E; Rodriguez, C; Fanara, J J

    1994-01-01

    D. buzzatii is a cactophilic species associated with several cactaceae in Argentina. This particular ecological niche implies that this species is faced with a non-uniform environment constituted by discrete and ephemeral breeding sites, which are colonized by a finite number of inseminated females. The genetic consequences of this population structure upon the second chromosome polymorphism were investigated by means of F-statistics in a natural endemic population of Argentina. The present study suggests that differentiation of inversion frequencies in third instar larvae among breeding sites has taken place mainly at random and selection is not operating to determine the structure of this population. The average number of parents breeding on a single pad seems to be similar to the number colonizing Opuntia ficus indica rotting cladodes in Carboneras, a derived population from Spain. There is no significant excess of heterokaryotypes within pads or in the population as a whole. The results obtained in the present study suggest that the potential role of selective versus stochastic factors relative to the among pad heterogeneity in the population here studied is different from that of the Spanish population previously reported. Potential mechanisms responsible for these differences are discussed.

  7. Inhibition of nitric oxide synthase expression in activated microglia and peroxynitrite scavenging activity by Opuntia ficus indica var. saboten.

    Science.gov (United States)

    Lee, Ming Hong; Kim, Jae Yeon; Yoon, Jeong Hoon; Lim, Hyo Jin; Kim, Tae Hee; Jin, Changbae; Kwak, Wie-Jong; Han, Chang-Kyun; Ryu, Jae-Ha

    2006-09-01

    Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO-), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. A butanol fraction obtained from 50% ethanol extracts of Opuntia ficus indica var. saboten (Cactaceae) stem (SK OFB901) and its hydrolysis product (SK OFB901H) inhibited the production of NO in LPS-activated microglia in a dose dependent manner (IC50 15.9, 4.2 microg/mL, respectively). They also suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells at higher than 30 microg/mL as observed by western blot analysis and RT-PCR experiment. They also inhibited the degradation of I-kappaB-alpha in activated microglia. Moreover, they showed strong activity of peroxynitrite scavenging in a cell free bioassay system. These results imply that Opuntia ficus indica may have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.

  8. Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers.

    Science.gov (United States)

    Bendhifi Zarroug, M; Baraket, G; Zourgui, L; Souid, S; Salhi Hannachi, A

    2015-02-13

    Opuntia ficus indica is one of the most economically important species in the Cactaceae family. Increased interest in this crop stems from its potential contribution to agricultural diversification, application in the exploitation of marginal lands, and utility as additional income sources for farmers. In Tunisia, O. ficus indica has been affected by drastic genetic erosion resulting from biotic and abiotic stresses. Thus, it is imperative to identify and preserve this germplasm. In this study, we focused on the use of random amplified microsatellite polymorphisms to assess genetic diversity among 25 representatives of Tunisian Opuntia species maintained in the collection of the National Institute of Agronomic Research of Tunisia. Seventy-two DNA markers were screened to discriminate accessions using 16 successful primer combinations. The high percentage of polymorphic band (100%), the resolving power value (5.68), the polymorphic information content (0.94), and the marker index (7.2) demonstrated the efficiency of the primers tested. Therefore, appropriate cluster analysis used in this study illustrated a divergence among the cultivars studied and exhibited continuous variation that occurred independently of geographic origin. O. ficus indica accessions did not cluster separately from the other cactus pear species, indicating that their current taxonomical classifications are not well aligned with their genetic variability or locality of origin.

  9. Convergence of a specialized root trait in plants from nutrient-impoverished soils: phosphorus-acquisition strategy in a nonmycorrhizal cactus.

    Science.gov (United States)

    Abrahão, A; Lambers, H; Sawaya, A C H F; Mazzafera, P; Oliveira, R S

    2014-10-01

    In old, phosphorus (P)-impoverished habitats, root specializations such as cluster roots efficiently mobilize and acquire P by releasing large amounts of carboxylates in the rhizosphere. These specialized roots are rarely mycorrhizal. We investigated whether Discocactus placentiformis (Cactaceae), a common species in nutrient-poor campos rupestres over white sands, operates in the same way as other root specializations. Discocactus placentiformis showed no mycorrhizal colonization, but exhibited a sand-binding root specialization with rhizosheath formation. We first provide circumstantial evidence for carboxylate exudation in field material, based on its very high shoot manganese (Mn) concentrations, and then firm evidence, based on exudate analysis. We identified predominantly oxalic acid, but also malic, citric, lactic, succinic, fumaric, and malonic acids. When grown in nutrient solution with P concentrations ranging from 0 to 100 μM, we observed an increase in total carboxylate exudation with decreasing P supply, showing that P deficiency stimulated carboxylate release. Additionally, we tested P solubilization by citric, malic and oxalic acids, and found that they solubilized P from the strongly P-sorbing soil in its native habitat, when the acids were added in combination and in relatively low concentrations. We conclude that the sand-binding root specialization in this nonmycorrhizal cactus functions similar to that of cluster roots, which efficiently enhance P acquisition in other habitats with very low P availability.

  10. Opuntia Extract Reduces Scar Formation in Rabbit Ear Model: A Randomized Controlled Study.

    Science.gov (United States)

    Fang, Quan; Huang, Chunlan; You, Chuangang; Ma, Shaolin

    2015-12-01

    The purpose of this article is to investigate the effect of Opuntia stricta H (Cactaceae) extract on suppression of hypertrophic scar on ventral surface wounds of rabbit ears. Full thickness skin defection was established in a rabbit ear to simulate hypertrophic scar. Opuntia extract was sprayed on the wounds in the experimental group, and normal saline was used in the control group. After the wounds healed with scar formation, the hypertrophic scar tissue was harvested on days 22, 39, and 54 for histological analysis. The expression of type I and type III collagen and matrix metalloproteinase-1 (MMP-1) were evaluated by immunohistochemistry and real-time quantitative polymerase chain reaction. The results indicated that the scar of the control group is more prominent compared with the opuntia extract group. The expression of type I collagen in the opuntia extract group was lower than the control group, while type III collagen in opuntia extract group gradually increased and exceeded control group. The expression of MMP-1 decreased in the opuntia extract group, while the control group increased over time, but the amount of MMP-1 was much higher than that in the control group on day 22. In conclusion, opuntia extract reduces hypertrophic scar formation by means of type I collagen inhibition, and increasing type III collagen and MMP-1.T he novel application of opuntia extract may lead to innovative and effective antiscarring therapies.

  11. Composição florística e estrutura da comunidade de epífitas vasculares em uma área de ecótono em Campo Mourão, PR, Brasil Floristic composition and structure of the vascular epiphyte community in a transition area at Campo Mourão, Paraná, Brazil

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    Henrique Cesar Lopes Geraldino

    2010-06-01

    Full Text Available O presente estudo foi realizado em uma área de ecótono de aproximadamente 30 hectares entre Floresta Estacional Semidecidual e Floresta Ombrófila Mista, localizada na Capela do Calvário, município de Campo Mourão, PR. Para a análise fitossociológica foram selecionados 80 forófitos. No levantamento total foram encontradas 61 espécies, 39 gêneros e 13 famílias de epífitas (10 de Pteridófitas e 51 de Magnoliófitas. As famílias mais ricas foram: Orchidaceae (38%, Bromeliaceae e Polypodiaceae (13%, Cactaceae (11% e Piperaceae (8%, que juntas compõem 83% das espécies amostradas. Os gêneros Tillandsia (Bromeliaceae e Peperomia (Piperaceae foram os mais ricos, com cinco espécies. A anemocoria foi constatada em 67% das espécies e 86% foram classificadas como holoepífitas verdadeiras. Das 61 espécies inventariadas, 43 ocorreram nas áreas de amostragem, sendo cinco em maior freqüência: Microgramma squamulosa, Pleopeltis angusta, Tillandsia loliacea, Tillandsia recurvata e Pecluma sicca. O índice de Shannon (H’ registrado para a área foi de 3,175 e a equabilidade (J 0,863. Foram encontradas nove espécies epifíticas restritas a apenas um forófito. Microgramma squamulosa foi a espécie mais importante em toda área amostral. O trecho de vegetação em área de interflúvio, por apresentar estágio avançado de desenvolvimento, teve riqueza superior à área de vegetação ribeirinha.This study was conducted in a transition area of approximately 30 hectares between Semideciduous Forest and Araucaria Forest located in the Capela do Calvário, at Campo Mourão, Paraná. For the phytosociological analysis 80 phorophytes were selected. The survey found 61 species, 39 genera and 13 families of epiphytes (10 pteridophytes and 51 magnoliophytes. The richest families were: Orchidaceae (38%, Bromeliaceae and Polypodiaceae (13%, Cactaceae (11% and Piperaceae (8%, which together make up 83% of the sampled species. Tillandsia (Bromeliaceae

  12. Uso e diversidade de plantas medicinais da Caatinga na comunidade rural de Laginhas, município de Caicó, Rio Grande do Norte (Nordeste do Brasil Use and diversity of medicinal plants from Caatinga in the rural community of Laginhas, Caicó Municipality, Rio Grande do Norte State (Northeast of Brazil

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    A.A Roque

    2010-03-01

    Full Text Available O presente trabalho teve como objetivo identificar as formas de uso de plantas medicinais nativas do bioma Caatinga, em comunidade rural no município de Caicó, Rio Grande do Norte (Nordeste do Brasil. Utilizaram-se entrevistas semi-estruturadas e estruturadas buscando informações, junto a especialistas locais, sobre o uso das plantas. São descritos os usos medicinais de 62 espécies, reportadas por 12 informantes (mateiros, rezadeiras, raizeiros, agricultores e donas-de-casa com idade superior a 35 anos. As famílias com maior representatividade na consulta foram Fabaceae (13 spp., Euphorbiaceae (6 spp. Cactaceae (3 spp. e Lamiaceae (3 spp.. Para revelar as espécies mais importantes foi considerado o grau de consenso entre as respostas dos informantes. A aroeira (Myracrodruon urundeuva Allemão e o cumaru (Amburana cearensis (Allemão A. C. Sm. destacaram-se como as espécies com o maior número de citações, sendo estas também as que obtiveram o maior número de indicações de usos terapêuticos. As cascas e as raízes foram as partes predominantemente consumidas. Os dados levantados por esta pesquisa evidenciaram uma diversidade de espécies da flora seridoense com potencial medicinal e reforçam a importância que a biodiversidade tem sobre as comunidades rurais, viabilizando o início do estudo de manejo da vegetação local.The present study aimed to identify the different uses of medicinal plants native to Caatinga biome in a rural community from Caicó Municipality, Rio Grande do Norte State, Brazil. Semi-structured and structured interviews with local specialists were used to collect information about the use of such plants. The medicinal uses of 62 species were described by 12 informants (woodsmen, faith healers, herb doctors, farmers and housewives older than 35 years. The most representative families reported in the survey were Fabaceae (13 spp, Euphorbiaceae (6 spp., Cactaceae (3 spp., and Lamiaceae (3 spp.. The degree of

  13. Evaluación del riesgo de extinción de Mammillaria pectinifera, cactácea endémica de la región de Tehuacán-Cuicatlán Assessment of extinction risk of Mammillaria pectinifera, an endemic cactus of the Tehuacán-Cuicatlán region

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    Pedro Luis Valverde

    2009-04-01

    Full Text Available Mammillaria pectinifera (Cactaceae es una especie endémica de la Región de Tehuacán-Cuicatlán en los estados de Puebla y Oaxaca. Aunque en la Norma Oficial Mexicana NOM-059-ECOL-2001 está considerada como amenazada y está incluida en el Apéndice I de la CITES, la información derivada de los escasos estudios ecológicos sobre esta especie revela serios riesgos de extinción de sus poblaciones. En el presente estudio se registra una evaluación del riesgo de extinción de M. pectinifera, para la que se empleó el protocolo del Método de Evaluación del Riesgo de Extinción de las Especies Silvestres en México (MER del Anexo I de la NOM-059-ECOL-2001, el cual se basa en 4 criterios para evaluar cualitativamente el riesgo de extinción de cualquier especie silvestre en el territorio nacional. Dicha evaluación se apoya en datos de campo de 7 poblaciones e información procedente de diversas fuentes. De acuerdo con los 12 puntos obtenidos del MER, se propone que M. pectinifera se considere en la categoría de especie en peligro de extinción (P en la NOM-059-ECOL-2001 y sea incluida de nuevo en la Lista Roja de la UICN. Finalmente se plantea una propuesta para mejorar la valoración del Criterio D del MER.Mammillaria pectinifera (Cactaceae is an endemic species from the Tehuacán-Cuicatlán region at the Mexican States of Puebla and Oaxaca. Although it is considered as a threatened species under Mexican law NOM-059-ECOL-2001, and it is already included in the CITES Appendix I, ecological studies reveal serious vulnerability to extinction of its populations. In this study, we conducted an extinction risk assessment for M. pectinifera based on the protocol of the Method for Evaluation of Risk of Extinction for Mexican Wild Species (MER from Appendix I of NOM-059-ECOL-2001, which is based on a qualitative assessment of 4 criteria for evaluating extinction risk of any wild species in the Mexican territory. The present assessment relies on data

  14. RECURSOS VEGETALES Y TECNOFACTURAS EN UN SITIO ARQUEOLÓGICO DE LA PUNA MERIDIONAL ARGENTINA, ÁREA CENTRO - SUR ANDINA

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    María F. Rodríguez

    2008-01-01

    Full Text Available El objetivo de esta investigación fue analizar el uso de los recursos vegetales para confeccionar tecnofacturas en el pasado prehistórico. Se estudiaron los artefactos recuperados en el sitio arqueológico Punta de la Peña 4, capas 0 - 4, datadas entre ca. 3900 - 500 años AP. El sitio está ubicado en las proximidades de la localidad de Antofagasta de la Sierra, Catamarca, Argentina (26° 11' 16'' S y 67° 20' 51.3'' O, a una altura de 3650 m s. m. Esta localidad pertenece a la Puna meridional y de acuerdo con las características ambientales, se integra a la Puna salada. Los objetivos específicos fueron identificar las especies vegetales utilizadas como materia prima y su área de procedencia, así como también evaluar el posible uso de cada uno de los artefactos hallados. Asimismo, se discutió la movilidad y los posibles intercambios socioeconómicos teniendo en cuenta los vegetales no locales utilizados. El análisis anatómico comparativo entre las especies actuales que crecen en el área de estudio y aquellas utilizadas en la confección de estas tecnofacturas permitió la identificación de las últimas, las cuales pertenecen a las familias Asteraceae, Cactaceae, Cyperaceae, Fabaceae, Juncaceae, Poaceae, Solanaceae y Typhaceae. Considerando las posibles áreas de procedencia de las especies arqueológicas se registró un menor grado de movilidad a grandes distancias durante el Holoceno tardío; en su lugar se instalan circuitos de movilidad pautada que obedecen a las actividades de pastoralismo que comienzan a desarrollarse.

  15. LEVANTAMENTO FLORÍSTICO DO ESTRATO ARBUSTIVO-ARBÓREO EM ÁREAS CONTÍGUAS DE CAATINGA NO CARIRI PARAIBANO

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    KALLIANNA DANTAS ARAUJO

    2010-01-01

    Full Text Available The purpose of this research was to make a floristic survey of the shrubby-arboreous stratum in three contiguous areas of Caatinga in the Cariri of Paraiba. The survey was accomplished in the "Estação Experimental Bacia Escola/UFPB", municipality of São João do Cariri, PB Brazilian Northeast. The experiment consisted of three areas of 3.2 ha, where three parallel transects had been established, in a distance of 20 m from each other, and there were marked ten parcels with equidistant systematic distribution (10 m x 10 m, having been sampled 30 parcels from each area. The floristic survey was carried through by the method of parcels. There was fulfilled a grouping analysis using the index of similarity of Jaccard. The number of parcels showed was enough to reflect the plantdiversity of the studied places; the families with highest number of species in the arboreous-shrubby stratum were Cactaceae and Euphorbiaceae; the species that had been common in the three areas, , were: Aspidosperma pyrifolium, Croton blanchetianus, Poincianella pyramidalis, Jatropha mollisssima, Malva sp., Pilosocereus gounellei, Spondias tuberosa and Opuntia palmadora. The arboreous habitat presented seven species, being higher to the shrubby component that was presented with five species. The more conspicuous arboreous elements had been: Poincianella pyramidalis (Caesalpiniaceae and Aspidosperma pyrifolium (Apocynaceae. The most important shrubs were: Croton blanchetianus and Jatropha mollisssima (Euphorbiaceae. The analysis made by the index of floristic similarity indicated that the similarity was considered high by the three area, because they presented a Jaccard index higher than 0.25.

  16. The ethnobotany of psychoactive plant use: a phylogenetic perspective

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    Nashmiah Aid Alrashedy

    2016-10-01

    Full Text Available Psychoactive plants contain chemicals that presumably evolved as allelochemicals but target certain neuronal receptors when consumed by humans, altering perception, emotion and cognition. These plants have been used since ancient times as medicines and in the context of religious rituals for their various psychoactive effects (e.g., as hallucinogens, stimulants, sedatives. The ubiquity of psychoactive plants in various cultures motivates investigation of the commonalities among these plants, in which a phylogenetic framework may be insightful. A phylogeny of culturally diverse psychoactive plant taxa was constructed with their psychotropic effects and affected neurotransmitter systems mapped on the phylogeny. The phylogenetic distribution shows multiple evolutionary origins of psychoactive families. The plant families Myristicaceae (e.g., nutmeg, Papaveraceae (opium poppy, Cactaceae (peyote, Convolvulaceae (morning glory, Solanaceae (tobacco, Lamiaceae (mints, Apocynaceae (dogbane have a disproportionate number of psychoactive genera with various indigenous groups using geographically disparate members of these plant families for the same psychoactive effect, an example of cultural convergence. Pharmacological traits related to hallucinogenic and sedative potential are phylogenetically conserved within families. Unrelated families that exert similar psychoactive effects also modulate similar neurotransmitter systems (i.e., mechanistic convergence. However, pharmacological mechanisms for stimulant effects were varied even within families suggesting that stimulant chemicals may be more evolutionarily labile than those associated with hallucinogenic and sedative effects. Chemically similar psychoactive chemicals may also exist in phylogenetically unrelated lineages, suggesting convergent evolution or differential gene regulation of a common metabolic pathway. Our study has shown that phylogenetic analysis of traditionally used psychoactive plants

  17. The Cacti Microbiome: Interplay between Habitat-Filtering and Host-Specificity.

    Science.gov (United States)

    Fonseca-García, Citlali; Coleman-Derr, Devin; Garrido, Etzel; Visel, Axel; Tringe, Susannah G; Partida-Martínez, Laila P

    2016-01-01

    Cactaceae represents one of the most species-rich families of succulent plants native to arid and semi-arid ecosystems, yet the associations Cacti establish with microorganisms and the rules governing microbial community assembly remain poorly understood. We analyzed the composition, diversity, and factors influencing above- and below-ground bacterial, archaeal, and fungal communities associated with two native and sympatric Cacti species: Myrtillocactus geometrizans and Opuntia robusta. Phylogenetic profiling showed that the composition and assembly of microbial communities associated with Cacti were primarily influenced by the plant compartment; plant species, site, and season played only a minor role. Remarkably, bacterial, and archaeal diversity was higher in the phyllosphere than in the rhizosphere of Cacti, while the opposite was true for fungi. Semi-arid soils exhibited the highest levels of microbial diversity whereas the stem endosphere the lowest. Despite their taxonomic distance, M. geometrizans and O. robusta shared most microbial taxa in all analyzed compartments. Influence of the plant host did only play a larger role in the fungal communities of the stem endosphere. These results suggest that fungi establish specific interactions with their host plant inside the stem, whereas microbial communities in the other plant compartments may play similar functional roles in these two species. Biochemical and molecular characterization of seed-borne bacteria of Cacti supports the idea that these microbial symbionts may be vertically inherited and could promote plant growth and drought tolerance for the fitness of the Cacti holobiont. We envision this knowledge will help improve and sustain agriculture in arid and semi-arid regions of the world.

  18. Targets of an invasive species: oviposition preference and larval performance of Cactoblastis cactorum (Lepidoptera: Pyralidae) on 14 North American opuntioid cacti.

    Science.gov (United States)

    Jezorek, Heather A; Stiling, Peter D; Carpenter, James E

    2010-12-01

    Cactoblastis cactorum Berg (Lepidoptera: Pyralidae), the cactus moth, is a well-known biological control agent of prickly pear cactus (Cactaceae: Opuntia Miller). The arrival of the moth in Florida and its subsequent spread through the southeastern United States poses a threat to opuntioid diversity in North America. Of particular concern are the ecological and economic impacts the moth could have in the southwestern United States and Mexico, where both native and cultivated Opuntia species are important resources. It is unknown which species would best support larval development if the moth were to spread further westward in North America. This study aimed to determine if ovipositing females demonstrate preferences for any of 14 common opuntioids native to or naturalized in Mexico and the southwestern United States; which of these opuntioids best support larval development; and if oviposition preference correlates with larval performance, as predicted by simple adaptive models. Results from a field experiment showed that female moths preferred O. engelmannii Salm-Dyck ex Engelmann variety linguiformis (Griffiths) Parfitt and Pinkava and O. engelmannii variety engelmannii for oviposition. A generalized linear model showed number of cladodes and degree of spininess to be significant predictors of oviposition activity. Results from a no-choice larval survival experiment showed Consolea rubescens (Salm-Dyck ex de Candolle.) Lemaire and O. streptacantha Lemaire to be the best hosts. Epidermal toughness was a significant predictor of most larval fitness parameters. In general, oviposition preference was not correlated with larval performance. A lack of co-evolutionary history between C. cactorum and North American opuntioid species may help explain this disconnect.

  19. Development and use of bioenergy feedstocks for semi-arid and arid lands.

    Science.gov (United States)

    Cushman, John C; Davis, Sarah C; Yang, Xiaohan; Borland, Anne M

    2015-07-01

    Global climate change is predicted to increase heat, drought, and soil-drying conditions, and thereby increase crop sensitivity to water vapour pressure deficit, resulting in productivity losses. Increasing competition between agricultural freshwater use and municipal or industrial uses suggest that crops with greater heat and drought durability and greater water-use efficiency will be crucial for sustainable biomass production systems in the future. Agave (Agavaceae) and Opuntia (Cactaceae) represent highly water-use efficient bioenergy crops that could diversify bioenergy feedstock supply yet preserve or expand feedstock production into semi-arid, abandoned, or degraded agricultural lands, and reclaim drylands. Agave and Opuntia are crassulacean acid metabolism species that can achieve high water-use efficiencies and grow in water-limited areas with insufficient precipitation to support traditional C3 or C4 bioenergy crops. Both Agave and Opuntia have the potential to produce above-ground biomass rivalling that of C3 and C4 crops under optimal growing conditions. The low lignin and high amorphous cellulose contents of Agave and Opuntia lignocellulosic biomass will be less recalcitrant to deconstruction than traditional feedstocks, as confirmed by pretreatments that improve saccharification of Agave. Refined environmental productivity indices and geographical information systems modelling have provided estimates of Agave and Opuntia biomass productivity and terrestrial sequestration of atmospheric CO2; however, the accuracy of such modelling efforts can be improved through the expansion of field trials in diverse geographical settings. Lastly, life cycle analysis indicates that Agave would have productivity, life cycle energy, and greenhouse gas balances comparable or superior to those of traditional bioenergy feedstocks, but would be far more water-use efficient.

  20. The Cacti microbiome: interplay between habitat-filtering and host specificity

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    Citlali eFonseca-Garcia

    2016-02-01

    Full Text Available Cactaceae represents one of the most species-rich families of succulent plants native to arid and semi-arid ecosystems, yet the associations Cacti establish with microorganisms and the rules governing microbial community assembly remain poorly understood. We analyzed the composition, diversity and factors influencing above- and below-ground bacterial, archaeal and fungal communities associated with two native and sympatric Cacti species: Myrtillocactus geometrizans and Opuntia robusta. Phylogenetic profiling showed that the composition and assembly of microbial communities associated with Cacti were primarily influenced by the plant compartment; plant species, site and season played only a minor role. Remarkably, bacterial and archaeal diversity was higher in the phyllosphere than in the rhizosphere of Cacti, while the opposite was true for fungi. Semi-arid soils exhibited the highest levels of microbial diversity whereas the stem endosphere the lowest. Despite their taxonomic distance, M. geometrizans and O. robusta shared most microbial taxa in all analyzed compartments. Influence of the plant host did only play a larger role in the fungal communities of the stem endosphere. These results suggest that fungi establish specific interactions with their host plant inside the stem, whereas microbial communities in the other plant compartments may play similar functional roles in these two species.Biochemical and molecular characterization of seed-borne bacteria of Cacti supports the idea that these microbial symbionts may be vertically inherited and could promote plant growth and drought tolerance for the fitness of the Cacti holobiont. We envision this knowledge will help improve and sustain agriculture in arid and semi-arid regions of the world.

  1. 常态下仙人掌科植物负离子释放量与其刺尖数量的相关性研究%Correlation between the spine number and the release of negative air ions by cactaceous in the natural state

    Institute of Scientific and Technical Information of China (English)

    丁旭玲; 张万超; 黄龙飞; 郑金贵; 吴仁烨; 邓传远

    2015-01-01

    通过对常态下仙人掌科(Cactaceae)植物释放负离子的研究,以期筛选出可产生较高浓度负离子的仙人掌科植物,为具生态效应的室内美化植物选择提供理论基础.常态下在密闭玻璃箱内(800 mm× 800 mm×800 mm)测定和比较了6种(含种下分类单位及品种)仙人掌科植物产生的负离子浓度并通过逐步去刺试验研究了艳珠球(Mammillaria spinosissima‘Pico’)刺的数量与负离子产生浓度的关系.结果表明,所有供试植物在自然状态下都能产生负离子,但产生负离子浓度均较低,且不同植物间产生负离子浓度有极显著性差异.以配对t检验结果来看,供试植物负离子产生浓度受空气中负离子浓度值影响显著.在逐步去刺试验中,艳珠球负离子产生浓度与刺的数量呈显著正相关.

  2. Pulse seedling recruitment on the population dynamics of a columnar cactus: Effect of an extreme rainfall event

    Science.gov (United States)

    Arroyo-Cosultchi, Gabriel; Golubov, Jordan; Mandujano, María C.

    2016-02-01

    Demographic studies on the Cactaceae have highlighted several threats which are clearly human induced (e.g., disturbance) or intrinsic to their biology (e.g., infrequent recruitment). Most demographic studies suggest that early life stages of germination and seedling recruitment are crucial and often a limitation for population growth. The population dynamics of Neobuxbaumia polylopha (DC) Backeb. was modeled for a three-year period to assess the contribution of the early life cycle stages on population growth rate (λ). Two annual size-classified matrix population models were constructed for standard analysis, applied a life table response experiment (LTRE) analysis to explore the contributions of demographic processes, plant size, and temporal variability (years) to λ, and changes in the matrix elements were simulated including a seed bank, and seed-to-seedling transition using observed and experimental data. The population growth rates for 2012-2013 and 2013-2014 were 0.9916 (0.9906-0.9929) and 1.0216 (1.011-1.0280) respectively, suggesting two opposite growth rates for the studied period. The increase in λ in 2013-2014 was driven primarily by the increased growth and seedling recruitment and survival of small individuals. The rate of recruitment was higher in 2013-2014 with a left-skewed stable size distribution. Elasticity values were high for matrix entries corresponding to individuals remaining in the same category (stasis), followed by growth, retrogression and fecundity. The simulations show that the seed bank has a minor effect in comparison with the seed-seedling transition which became the population bottleneck under the assumption that seeds are not limited, so programs designed to preserve N. polylopha populations must focus on seedling establishment.

  3. Tissue culture of ornamental cacti

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    Eugenio Pérez-Molphe-Balch

    2015-12-01

    Full Text Available Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

  4. Recursos florísticos de la cuenca baja del río mayo, Sonora

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    Julio César Duarte Ruiz

    2014-06-01

    Full Text Available En México se presentan prácticamente todos los grandes tipos de vegetación que existen en el mundo y su distribución a menudo es compleja. El objetivo del trabajo consistió en realizar un listado de la flora silvestre ribereña del Río Mayo, localizado en el sur del estado de Sonora, para conocer su composición. La recolección de especímenes se realizó mediante la técnica de intercepción o línea de Canfield (1941, en 15 sitios con dos repeticiones sumando 30 transectos. Se encontraron un total de 40 familias, 79 géneros y 99 especies. Las familias mejor representadas fueron: Leguminoseae, Cactaceae, Gramineae, Compositae, Chenopodiaceae, Salicaceae, Solanaceae, Euphorbiaceae, Amaranthaceae y Asteraceae. La dominante fue Leguminoseae con vegetación de bosque espinoso compuesta por Acacia cymbispina, Acacia farnesiana, Parkinsonia aculeata y Prosopis juliflora. Resulta perceptible la pérdida de flora debido al cambio de uso de suelo. Especies representativas de la zona riparia como Populus fremontii y Salix bonplandiana están desapareciendo por alteración del ecosistema. Poblaciones de mangle como Rhizophora mangle y Conocarpus erectus están disminuidas. El área estudiada es rica en especies a pesar de la perturbación. La diversidad varía de acuerdo al gradiente altitudinal y el número de especies guarda relación con los géneros y familias recolectadas. La agricultura, acuicultura, ganadería, la industria pesquera y la urbanización, son los giros que causan mayor afectación en los ecosistemas

  5. Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica

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    Romulo Marino Llamoca-Zárate

    1999-01-01

    Full Text Available We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.Nós demonstramos a expressão transiente do gene GUS nas células do meristema apical de Opuntia ficus-indica. A introdução do DNA nas células foi realizada através de um sistema de biolística PDS-1000He da Bio-Rad Laboratories. Para transformação, partículas de tungstenio com diâmetro de 1,3 µm foram cobertas com DNA e a distância entre o disco das partículas e o tecido alvo foi de 7,5cm, a pressão de tiro foi 1200 psi. Esta é a primeira demonstração que o sistema de biolística de transformação pode ser usado para a expressão de transgenes nas cactáceas.

  6. The ethnobotany of psychoactive plant use: a phylogenetic perspective

    Science.gov (United States)

    2016-01-01

    Psychoactive plants contain chemicals that presumably evolved as allelochemicals but target certain neuronal receptors when consumed by humans, altering perception, emotion and cognition. These plants have been used since ancient times as medicines and in the context of religious rituals for their various psychoactive effects (e.g., as hallucinogens, stimulants, sedatives). The ubiquity of psychoactive plants in various cultures motivates investigation of the commonalities among these plants, in which a phylogenetic framework may be insightful. A phylogeny of culturally diverse psychoactive plant taxa was constructed with their psychotropic effects and affected neurotransmitter systems mapped on the phylogeny. The phylogenetic distribution shows multiple evolutionary origins of psychoactive families. The plant families Myristicaceae (e.g., nutmeg), Papaveraceae (opium poppy), Cactaceae (peyote), Convolvulaceae (morning glory), Solanaceae (tobacco), Lamiaceae (mints), Apocynaceae (dogbane) have a disproportionate number of psychoactive genera with various indigenous groups using geographically disparate members of these plant families for the same psychoactive effect, an example of cultural convergence. Pharmacological traits related to hallucinogenic and sedative potential are phylogenetically conserved within families. Unrelated families that exert similar psychoactive effects also modulate similar neurotransmitter systems (i.e., mechanistic convergence). However, pharmacological mechanisms for stimulant effects were varied even within families suggesting that stimulant chemicals may be more evolutionarily labile than those associated with hallucinogenic and sedative effects. Chemically similar psychoactive chemicals may also exist in phylogenetically unrelated lineages, suggesting convergent evolution or differential gene regulation of a common metabolic pathway. Our study has shown that phylogenetic analysis of traditionally used psychoactive plants suggests

  7. Vascular epiphytic component in an urban forest fragment in Criciuma, Santa Catarina, Brazil

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    Telma Elyta Vilhalba Azeredo

    2013-06-01

    Full Text Available This study aimed to conduct a floristic and phytosociological survey, as well as analyze the spatial distribution of the vascular epiphytic component in an urban forest fragment belonging to the submontane dense ombrophilous forest in the town of Criciuma, Santa Catarina, Brazil. In addition, information on the ecological groups of epiphytic species and the strategies for pollination and dispersal were also presented. One sampled 60 trees as phorophytes with DBH ≥ 10 cm, through the point-centered quarter method, and the expeditious walking method was used for recording the epiphytic species which weren’t sampled in the phorophytes through the phytosociological method. The frequency was evaluated having the occurrence of epiphytes in the phorophytes and the segments on the bole and crown as a basis. One recorded the presence of epiphytes in the phorophytes in the segments on the bole and crown. One found 65 species distributed into 39 genera and 14 families, out of which 49 were sampled in the phytosociological survey and the remaining ones in the walking survey. Bromeliaceae showed the highest richness, followed by Orchidaceae, and Cactaceae. Tillandsia recurvata (L. L. was firstly mentioned in the southern state. The specific diversity was estimated as H’ = 3.33 and evenness (E was equal to 0.86. The ecological group of holoepiphytes was the most representative one in the area under study. Entomophily and anemochory were the prevailing strategies for pollination and dispersal, respectively. In the phytosociological survey, the number of epiphytic species in the phorophytes ranged from 0 to 21. The highest importance values were those related to Rhipsalis teres (Vell. Steud. and Microgramma vacciniifolia (Langsd. & Fisch. Copel.

  8. Presence of Multidrug-Resistant Shiga Toxin-Producing Escherichia coli, Enteropathogenic E. coli and Enterotoxigenic E. coli, on Raw Nopalitos (Opuntia ficus-indica L.) and in Nopalitos Salads from Local Retail Markets in Mexico.

    Science.gov (United States)

    Gómez-Aldapa, Carlos A; Cerna-Cortes, Jorge F; Rangel-Vargas, Esmeralda; Torres-Vitela, Mdel Refugio; Villarruel-López, Angelica; Gutiérrez-Alcántara, Eduardo J; Castro-Rosas, Javier

    2016-05-01

    The presence of multidrug-resistant pathogenic bacteria in food is a significant public health concern. Diarrheagenic Escherichia coli pathotypes (DEPs) are foodborne bacteria. In Mexico, DEPs have been associated with diarrheal illness. There is no information about the presence of multidrug-resistant DEPs on fresh vegetables and in cooked vegetable salads in Mexico. "Nopalitos" (Opuntia ficus-indica L.) is a Cactacea extensively used as a fresh green vegetable throughout Mexico. The presence of generic E. coli and multidrug-resistant DEPs on raw whole and cut nopalitos and in nopalitos salad samples was determined. One hundred raw whole nopalitos (without prickles) samples, 100 raw nopalitos cut into small square samples, and 100 cooked nopalitos salad samples were collected from markets. Generic E. coli was determined using the most probable number procedures. DEPs were identified using two multiplex polymerase chain reaction procedures. Susceptibility to 16 antibiotics was tested for the isolated DEP strains by standard test. Of the 100 whole nopalitos samples, 100 cut nopalitos samples, and 100 nopalitos salad samples, generic E. coli and DEPs were identified, respectively, in 80% and 10%, 74% and 10%, and 64% and 8%. Eighty-two DEP strains were isolated from positive nopalitos samples. The identified DEPs included Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). All isolated strains exhibited resistance to at least six antibiotics. To the best of our knowledge, this is the first report of the presence of multidrug-resistant and antibiotic resistance profiles of STEC, ETEC, and EPEC on raw nopalitos and in nopalitos salads in Mexico.

  9. Ecophysiological and Anatomical Mechanisms behind the Nurse Effect: Which Are More Important? A Multivariate Approach for Cactus Seedlings

    Science.gov (United States)

    Delgado-Sánchez, Pablo; Yáñez-Espinosa, Laura; Jiménez-Bremont, Juan Francisco; Chapa-Vargas, Leonardo; Flores, Joel

    2013-01-01

    Background Cacti establish mostly occurs under the canopy of nurse plants which provide a less stressful micro-environment, although mechanisms underlying this process are unknown. The impact of the combination of light and watering treatments on Opuntia streptacantha (Cactaceae) seedlings was examined. Methods/Principal Findings Ecophysiological [titratable acidity, osmotic potential (‘solute potential’, Ψs), relative growth rate (RGR) and their components (NAR, SLA, and LWR)], anatomical (chloroplast density, chloroplast frequency, and cell area), and environmental [photosynthetic photon flux density (PPFD) and air temperature] sets of variables were analyzed, assessing relationships between them and measuring the intensity of the relationships. Three harvests were carried out at days 15, 30, and 45. Ψs and acidity content were the most important responses for seedling establishment. The main anatomical and environmental variables were chloroplast density and water availability, respectively. Opuntia streptacantha seedlings establish better in the shade-watering treatment, due to higher Ψs and acidity, unaffected chloroplasts, and lower PPFD. In addition, the chloroplasts of cells under high-light and non-watering treatment were clumped closer to the center of the cytosol than those under shade-drought, to avoid photoinhibition and/or to better distribute or utilize the penetrating light in the green plant tissue. Conclusions Opuntia seedlings grow better under the shade, although they can tolerate drought in open spaces by increasing and moving chloroplasts and avoiding drastic decreases in their Ψs. This tolerance could have important implications for predicting the impact of climate change on natural desert regeneration, as well as for planning reforestation-afforestation practices, and rural land uses. PMID:24312310

  10. Environmental regulation of carbon isotope composition and crassulacean acid metabolism in three plant communities along a water availability gradient.

    Science.gov (United States)

    Ricalde, M Fernanda; Andrade, José Luis; Durán, Rafael; Dupuy, Juan Manuel; Simá, J Luis; Us-Santamaría, Roberth; Santiago, Louis S

    2010-12-01

    Expression of crassulacean acid metabolism (CAM) is characterized by extreme variability within and between taxa and its sensitivity to environmental variation. In this study, we determined seasonal fluctuations in CAM photosynthesis with measurements of nocturnal tissue acidification and carbon isotopic composition (δ(13)C) of bulk tissue and extracted sugars in three plant communities along a precipitation gradient (500, 700, and 1,000 mm year(-1)) on the Yucatan Peninsula. We also related the degree of CAM to light habitat and relative abundance of species in the three sites. For all species, the greatest tissue acid accumulation occurred during the rainy season. In the 500 mm site, tissue acidification was greater for the species growing at 30% of daily total photon flux density (PFD) than species growing at 80% PFD. Whereas in the two wetter sites, the species growing at 80% total PFD had greater tissue acidification. All species had values of bulk tissue δ(13)C less negative than -20‰, indicating strong CAM activity. The bulk tissue δ(13)C values in plants from the 500 mm site were 2‰ less negative than in plants from the wetter sites, and the only species growing in the three communities, Acanthocereus tetragonus (Cactaceae), showed a significant negative relationship between both bulk tissue and sugar δ(13)C values and annual rainfall, consistent with greater CO(2) assimilation through the CAM pathway with decreasing water availability. Overall, variation in the use of CAM photosynthesis was related to water and light availability and CAM appeared to be more ecologically important in the tropical dry forests than in the coastal dune.

  11. Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice

    Science.gov (United States)

    Madrigal-Santillán, Eduardo; García-Melo, Fernando; Morales-González, José A.; Vázquez-Alvarado, Patricia; Muñoz-Juárez, Sergio; Zuñiga-Pérez, Clara; Sumaya-Martínez, Maria Teresa; Madrigal-Bujaidar, Eduardo; Hernández-Ceruelos, Alejandra

    2013-01-01

    Plants belonging to the genus Opuntia spp. are the most abundant of the Cactaceae family, grown throughout America and the Mediterranean central area. Its fruit, known as cactus pear or prickly pear, is an oval berry grouped in different colors. Some studies have shown its antioxidant activities which may help in preventing chronic pathologies such as diabetes. The purpose of the study was to evaluate the antioxidant capacity of three varieties of prickly pear juice (red-purple, white-green and yellow-orange) in five different concentrations (100, 250, 500, 750, and 1000 mg/mL) by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) colorimetric method, selecting the best variety to determine its anticlastogenic potential against methyl methanesulfonate (MMS). The results indicate that the highest antioxidant was found in the juice of the prickly pear red-purple variety (PPRP), in all concentrations. Its anticlastogenic potential was therefore evaluated with a micronucleus assay. The experiment was run over two weeks. A negative control was included along with a positive control with MMS (40 mg/kg), a group of mice treated with PPRP (25 mL/kg), and three groups with PPRP (in doses of 25, 16.5 and 8.3 mL/kg) plus the mutagen. The PPRP was administered daily by oral gavage and the MMS was injected intraperitoneally five days prior to the end of the experiment. Blood samples were obtained at 0, 24, 48, 72 and 96 h in order to determine the frequency of micronucleated polychromatic erythrocytes (MNPE). The results indicated that PPRP is not a genotoxic agent, on the contrary, it may reduce the number of MNPE. In this regard, the PPRP showed an anticlastogenic effect directly proportional to its concentrations. Thus, the highest protection was obtained with a concentration of 25 mL/kg after 48 h of treatment. PMID:24145870

  12. Water Relations, Diurnal Acidity Changes, and Productivity of a Cultivated Cactus, Opuntia ficus-indica1

    Science.gov (United States)

    Acevedo, Edmundo; Badilla, Ignacio; Nobel, Park S.

    1983-01-01

    Physiological responses of the Crassulacean acid metabolism (CAM) plant Opuntia ficus-indica (Cactaceae) were studied on a commercial plantation in central Chile. Young cladodes (flattened stems) and flower buds exhibited daytime stomatal opening, whereas mature cladodes and fruit exhibited the nocturnal stomatal opening characteristic of CAM plants. Severe water stress suppressed the nocturnal stomatal opening by mature cladodes, but their high water vapor conductance occurring near dawn was not affected. Nocturnal acidity increases were not as sensitive to water stress as was the nocturnal stomatal opening. The magnitude of the nocturnal acidity increases depended on the total daily photosynthetically active radiation (PAR), being 90% PAR-saturated at 27 moles per square meter per day for a mean nighttime air temperature of 5°C and at 20 moles per square meter per day for 18°C. Inasmuch as the PAR received on unshaded vertical surfaces averaged about 21 moles per square meter per day, nocturnal acidity increases by the cladodes were on the verge of being PAR-limited in the field. The net assimilation rate, which was positive throughout the year, annually averaged 3.4 grams per square meter per day for 1.0- and 2.0-year-old plants. Plants that were 5.4 years old had 7.2 square meters of cladode surface area (both sides) and an annual dry weight productivity of 13 megagrams (metric tons) per hectare per year when their ground cover was 32%. This substantial productivity for a CAM plant was accompanied by the highest nocturnal acidity increase so far observed in the field, 0.78 mole H+ per square meter. PMID:16663084

  13. Ethnobotanical study of useful vegetal species in two rural communities in the semi-arid region of Paraíba state (Northeastern Brazil.

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    Cleilton Marques Alves

    2014-04-01

    Full Text Available Trying to understand the relationship between man/natural resources, from ethonobotanical studies, this study aimed to estimate the use value of vegetal species in two Caatinga areas in the Cariri of Paraíba state, besides knowing the multiple uses of plants by the residents of rural communities of Brito (Queimadas - PB and Lagoa Salgada (Montadas, Areial, Pocinhos - PB. Semi-structured interviews were conducted with local specialists and the community. It was used by the specialists the technique “snow ball” and the technique “guided tour”. It was identified 77 species, being 40 in Brito community and 37 in Lagoa Salgada community, all of them distributed into use categories, since food up to technological uses. Seven species had the higher use value: 1: Croton blanchetianus, Mimosa caesalpinefolia, Prosopis julifora, Mimosa tenuiflora, Opuntia ficus-indica, Aspidosperma pyrifolium and Myracrodruon urundeuva. The used categories were equal between the two communities and among the general informants and local specialists, which show great resemblance of use and their preference for certain species. The lowest cited species were those for food and domestic building purposes, and the most used were those ones for medicinal use. The most cited species are Cactaceae, Euphorbiaceae and Fabaceae. Regarding the species, the most valued in both communities were Croton blanchetianus, Pilosocereus gounellei, Mimosa tenuiflora and Prosopis julifora.  Although the current communities have a great dependence of the local flora for surviving, the extraction and the lack of conservation of species are notorious in both communities.

  14. Differences in tolerance to host cactus alkaloids in Drosophila koepferae and D. buzzatii.

    Science.gov (United States)

    Soto, Ignacio M; Carreira, Valeria P; Corio, Cristian; Padró, Julián; Soto, Eduardo M; Hasson, Esteban

    2014-01-01

    The evolution of cactophily in the genus Drosophila was a major ecological transition involving over a hundred species in the Americas that acquired the capacity to cope with a variety of toxic metabolites evolved as feeding deterrents in Cactaceae. D. buzzatii and D. koepferae are sibling cactophilic species in the D. repleta group. The former is mainly associated with the relatively toxic-free habitat offered by prickly pears (Opuntia sulphurea) and the latter has evolved the ability to use columnar cacti of the genera Trichocereus and Cereus that contain an array of alkaloid secondary compounds. We assessed the effects of cactus alkaloids on fitness-related traits and evaluated the ability of D. buzzatii and D. koepferae to exploit an artificial novel toxic host. Larvae of both species were raised in laboratory culture media to which we added increasing doses of an alkaloid fraction extracted from the columnar cactus T. terschekii. In addition, we evaluated performance on an artificial novel host by rearing larvae in a seminatural medium that combined the nutritional quality of O. sulphurea plus amounts of alkaloids found in fresh T. terschekii. Performance scores in each rearing treatment were calculated using an index that took into account viability, developmental time, and adult body size. Only D. buzzatii suffered the effects of increasing doses of alkaloids and the artificial host impaired viability in D. koepferae, but did not affect performance in D. buzzatii. These results provide the first direct evidence that alkaloids are key determinants of host plant use in these species. However, the results regarding the artificial novel host suggest that the effects of alkaloids on performance are not straightforward as D. koepferae was heavily affected. We discuss these results in the light of patterns of host plan evolution in the Drosophila repleta group.

  15. Separation of polar betalain pigments from cacti fruits of Hylocereus polyrhizus by ion-pair high-speed countercurrent chromatography.

    Science.gov (United States)

    Wybraniec, Sławomir; Stalica, Paweł; Jerz, Gerold; Klose, Bettina; Gebers, Nadine; Winterhalter, Peter; Spórna, Aneta; Szaleniec, Maciej; Mizrahi, Yosef

    2009-10-09

    Polar betacyanin pigments together with betaxanthins from ripe cactus fruits of Hylocereus polyrhizus (Cactaceae) were fractionated by means of preparative ion-pair high-speed countercurrent chromatography (IP-HSCCC) also using the elution-extrusion (EE) approach for a complete pigment recovery. HSCCC separations were operated in the classical 'head-to-tail' mode with an aqueous mobile phase. Different CCC solvent systems were evaluated in respect of influence and effectiveness of fractionation capabilities to separate the occurring pigment profile of H. polyrhizus. For that reason, the additions of two different volatile ion-pair forming perfluorinated carboxylic acids (PFCA) were investigated. For a direct comparison, five samples of Hylocereus pigment extract were run on preparative scale (900 mg) in 1-butanol-acetonitrile-aqueous TFA 0.7% (5:1:6, v/v/v) and the modified systems tert.-butyl methyl ether-1-butanol-acetonitrile-aqueous PFCA (2:2:1:5, v/v/v/v) using 0.7% and 1.0% trifluoroacetic acid (TFA) or heptafluorobutyric acid (HFBA) in the aqueous phase, respectively. The chemical affinity to the organic stationary CCC solvent phases and in consequence the retention of these highly polar betalain pigments was significantly increased by the use of the more lipophilic fluorinated ion-pair reagent HFBA instead of TFA. The HFBA additions separated more effectively the typical cacti pigments phyllocactin and hylocerenin from betanin as well as their iso-forms. Unfortunately, similar K(D) ratios and selectivity factors alpha around 1.0-1.1 in all tested solvent systems proved that the corresponding diastereomers, 15S-type pigments cannot be resolved from the 15R-epimers (iso-forms). Surprisingly, additions of the stronger ion-pair reagent (HFBA) resulted in a partial separation of hylocerenin from phyllocactin which were not resolved in the other solvent systems. The pigments were detected by means of HPLC-DAD and HPLC-electrospray ionization-MS using also

  16. 仙人掌的微繁殖%Micropropagation of Opuntia dillenii (Ker-Gawl.) Haw.

    Institute of Scientific and Technical Information of China (English)

    程磊; 胡宋英

    2003-01-01

    A protocol is described for rapid in vitro propagation of the valuable chylocaula Opuntia dillenii(Ker-Gawl.)Haw. in vitro. The influence of various combinations of growth regulators,the physical stage of explants and the concentration of the macroelements were also evaluated. It was found that bud variation was dependent on BA supply,the synergistic combination of 5.0 mg/L and 0.1 mg/L IBA induced the optimum frequency(5.3 buds/stem segment). Stem segment with longitudinal split was prior to whole segment on bud induction. The concentration of Ca2+,Mg2+ affected root number and length,but had no effects on plantlet height and growth. Though root length of plantlets declined with increased NAA concentration,0.2 mg/L NAA promoted root number. Considering together,the best rooting medium was half-strength MS. It was observed that sprout tuber was superior to single nodal plate on bud number as well as bud growth. Some morphological variations exhibited in the transferred test-tube plants. This micropropagation procedure may provide the basis for improvement of this chylocaula and was beneficial to the tissue culture of other plants of Cactaceae.%成功建立了仙人掌离体快繁的实验体系,并且对影响微繁殖的一些因素,诸如激素组合、外植体的物理状态、大量元素的含量等进行了研究.结果表明:BA对仙人掌芽增殖具明显作用,MS+BA 5.0 mg/L+IBA 0.1 mg/L为最适增殖培养基;接种方式实验表明劈接优于整棵.钙、镁离子浓度对试管苗生长没有影响,但影响生根数及根长;NAA抑制根的伸长,但一定浓度可促进生根.总体而言,最适的生根培养基为1/2 MS.同时发现块接比单芽接具有优势.试管苗在形态上出现一些变异.实验结果对仙人掌科其它植物的快速繁殖具有参考意义.

  17. Morfoanatomia vegetativa de Opuntia brasiliensis (Willd Haw / Vegetative morphology and anatomy of Opuntia brasiliensis (Willd Haw

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    Camila Firmino de Azevedo

    2013-01-01

    Full Text Available Opuntia brasiliensis (Willd Haw. é uma Cactaceae nativa do Brasil utilizada como ornamental, que apresenta caracteres morfológicos semelhantes a outras espécies do gênero, o que dificulta sua identificação. Dessa forma, foi realizado uma descrição morfoanatômica de O. brasiliensis com o objetivo de ampliar o conhecimento sobre a organização estrutural do gênero e fornecer subsídios para a identificação da espécie, bem como observar caracteres importantes na adaptação a ambientes xerófilos. Foram feitas secções transversais e paradérmicas da raiz, caule principal e cladódios com espécimes coletados na zona rural da cidade de Serra Branca (PB, localizada na região do cariri ocidental. O. brasiliensis apresenta raiz ramificada e caule clorofilado formando um eixo principal de onde partem inúmeros cladódios delgados. Observamse pelos e epiderme unisseriada irregular na raiz, que possui córtex formado por parênquima, seguido de endoderme multisseriada e periciclo. No cilindro central da raiz, ocorrem tecidos vasculares formando cinco pólos seguidos de medula. O caule e o cladódio apresentam epiderme com paredes sinuosas, em que encontram-se estômatos paralelocíticos que apresentam câmara subestomática; e na camada subsequente, hipoderme com grande quantidade de drusas de oxalato de cálcio. O córtex é formado por parênquimas clorofiliano e aquífero, onde observam-se feixes vasculares, com raios de floema voltados para o lado externo, seguido de xilema helicoidal. Porém, nos cladódios, o parênquima clorofiliano é bem mais denso e os feixes vasculares são dispostos irregularmente, enquanto no caule estão distribuídos no sentido radial. O. brasiliensis apresenta várias características importantes na sua identificação, bem como adaptações estruturais a ambientes xerófilos

  18. Introduced and invasive cactus species: a global review

    Science.gov (United States)

    Novoa, Ana; Le Roux, Johannes J.; Robertson, Mark P.; Wilson, John R.U.; Richardson, David M.

    2015-01-01

    Understanding which species are introduced and become invasive, and why, are central questions in invasion science. Comparative studies on model taxa have provided important insights, but much more needs to be done to unravel the context dependencies of these findings. The cactus family (Cactaceae), one of the most popular horticultural plant groups, is an interesting case study. Hundreds of cactus species have been introduced outside their native ranges; a few of them are among the most damaging invasive plant species in the world. We reviewed the drivers of introductions and invasions in the family and seek insights that can be used to minimize future risks. We compiled a list of species in the family and determined which have been recorded as invasive. We also mapped current global distributions and modelled the potential global distributions based on distribution data of known invasive taxa. Finally, we identified whether invasiveness is phylogenetically clustered for cacti and whether particular traits are correlated with invasiveness. Only 57 of the 1922 cactus species recognized in this treatment have been recorded as invasive. There are three invasion hotspots: South Africa (35 invasive species recorded), Australia (26 species) and Spain (24 species). However, there are large areas of the world with climates suitable for cacti that are at risk of future invasion—in particular, parts of China, eastern Asia and central Africa. The invasive taxa represent an interesting subset of the total species pool. There is a significant phylogenetic signal: invasive species occur in 2 of the 3 major phylogenetic clades and in 13 of the 130 genera. This phylogenetic signal is not driven by human preference, i.e. horticultural trade, but all invasive species are from 5 of the 12 cactus growth forms. Finally, invasive species tend to have significantly larger native ranges than non-invasive species, and none of the invasive species are of conservation concern in their

  19. Differential distribution of amino acids in plants.

    Science.gov (United States)

    Kumar, Vinod; Sharma, Anket; Kaur, Ravdeep; Thukral, Ashwani Kumar; Bhardwaj, Renu; Ahmad, Parvaiz

    2017-03-15

    Plants are a rich source of amino acids and their individual abundance in plants is of great significance especially in terms of food. Therefore, it is of utmost necessity to create a database of the relative amino acid contents in plants as reported in literature. Since in most of the cases complete analysis of profiles of amino acids in plants was not reported, the units used and the methods applied and the plant parts used were different, amino acid contents were converted into relative units with respect to lysine for statistical analysis. The most abundant amino acids in plants are glutamic acid and aspartic acid. Pearson's correlation analysis among different amino acids showed that there were no negative correlations between the amino acids. Cluster analysis (CA) applied to relative amino acid contents of different families. Alismataceae, Cyperaceae, Capparaceae and Cactaceae families had close proximity with each other on the basis of their relative amino acid contents. First three components of principal component analysis (PCA) explained 79.5% of the total variance. Factor analysis (FA) explained four main underlying factors for amino acid analysis. Factor-1 accounted for 29.4% of the total variance and had maximum loadings on glycine, isoleucine, leucine, threonine and valine. Factor-2 explained 25.8% of the total variance and had maximum loadings on alanine, aspartic acid, serine and tyrosine. 14.2% of the total variance was explained by factor-3 and had maximum loadings on arginine and histidine. Factor-4 accounted 8.3% of the total variance and had maximum loading on the proline amino acid. The relative content of different amino acids presented in this paper is alanine (1.4), arginine (1.8), asparagine (0.7), aspartic acid (2.4), cysteine (0.5), glutamic acid (2.8), glutamine (0.6), glycine (1.0), histidine (0.5), isoleucine (0.9), leucine (1.7), lysine (1.0), methionine (0.4), phenylalanine (0.9), proline (1.1), serine (1.0), threonine (1

  20. "Oasis de neblina" en los cerros costeros del sur de Iquique, región de Tarapacá, Chile, durante el evento El Niño 1997-1998 Fog oases during the El Niño Southern Oscillation 1997-1998, in the coastal hills south of Iquique, Tarapacá region, Chile

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    MÉLICA MUÑOZ-SCHICK

    2001-06-01

    . A total of 72 vascular plant species were registered, contributing to the area´s floristic knowledge, where till now only about 20 species were registered. Three species are new records for the chilean flora: Nolana adansonii (Roem. et Schult. Johnst., Solanum cfr. montanum L. and Ophryosporus cfr. floribundus (D.C. King et Rob., and another one Alstroemeria lutea (Muñoz 2000 is a new species. This last one, together with Eulychnia aricensis Ritter, Pyrrhocactus saxifragus Ritter (both Cactaceae and Nolana intonsa Johnst. (Nolanaceae are local endemics. Of the total of registered species, 43 % are endemics, 52.7 % are non endemic natives and a low percentage (4.1 % are foreign species; 47.2 % share their distribution with Perú. The families Asteraceae, Nolanaceae and Solanaceae, are the best represented in the area, each one with more than seven species. Information about special climatic conditions due to El Niño 1997-1998 event is discussed in relationship to the development of the flora and vegetation. In spite of a non significant occurrence of rainfall events, an increase in temperature and in the fog's water content is detected. These conditions favor the development of more exuberant vegetation and the expansion of the distribution range of some floristic elements

  1. Descripción de los estados adultos e inmaduros y aspectos bioecológicos de Heterothrips cacti (Thysanoptera: Heterothripidae Description of adult and immature stages and bioecological aspects of Heterothrips cacti (Thysanoptera: Heterothripidae

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    María I. Zamar

    2011-06-01

    . hildmannii flowering season in 2006-2007. The sample consisted of five flowers of each condition of development collected randomly. In order to know the host plants and the distribution of H. cacti, periodic samplings of the wild vegetation were carried out in the Prepuna and Puna of Jujuy province. The biological studies were performed in laboratory placing flowers of O. sulphurea with eggs of H. cacti in rearing cages in order to control their life cycle evolution. Heterothrips cacti is an anthophilous, oligophagous species associated to cactaceae, widely distributed in the Prepuna and Puna of Jujuy province. The biology of H. cacti is correlated with the one of its host plant, O. sulphurea, presenting two generations per year. Likewise, its life cycle is associated with the evolution of the cactaceous flower. The time of development of the immature stages of the first and second generation was 45 ± 1.5 days and 287 ±3 days respectively. The stage of development through which the species undergoes the period between inflorescences is larva II quiescent wrapped in a silk thread cocoon on the floor. At 5 ºC and 0 hrs light the quiescent state of H. cacti is inhibited. The incidence of larvi-pupal parasitoid Ceranisus sp. during the second generation of H. cacti was recorded.

  2. Propagation in Schlumbergera truncata (Haw: alternatives to the use of xaxim = Propagação de Schlumbergera truncata (Haw: alternativas ao uso do xaxim

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    Rafael Fonseca Zanotti

    2015-10-01

    Full Text Available The ornamental cactus, Schlumbergera truncata, is propagated by cuttings, with xaxim (Dicksonia sellowiana being one of the substrates used. However, xaxim is endangered, and it has become necessary to evaluate alternative substrates. The use of suitable substrates and of substances that promote organogenesis, such as auxins, aid in the process of plant propagation. The aim of this study therefore, was to evaluate the development of cuttings of S. truncata in different substrates, and the effect of IBA on organogenesis. Six treatments were carried out, consisting of a combination of three substrates (powdered xaxim, powdered coconut and a commercial substrate, and two rates of IBA (0 and 600 mg L-1. Twenty cuttings were used per treatment, distributed over five replications of four cuttings, in a completely randomised design and a 3 x 2 factorial (substrates x AIB. There was no significant interaction between the factors, which were evaluated separately. For the powdered xaxim substrate, the commercial substrate and the powdered coconut, the cuttings displayed 3.0, 3.7 and 1.3 shoots per plant respectively. After exposure to a solution of IBA of 0 or 600 mg L-1, the cuttings presented 2.2 and 3.1 shoots per plant respectively. The substrate of powdered xaxim and the commercial substrate are both effective in the propagation of S. truncata. As the xaxim, found in the Atlantic Forest, is an endangered species, the use of a commercial substrate is recommended, with IBA as a growth regulator to promote an increase in budding. = A Schlumbergera truncata é uma cactaceae ornamental, propagada por estacas, e o xaxim é um dos substratos utilizados. Devido à ameaça de extinção do xaxim, torna-se necessário avaliar substratos alternativos. A utilização de substratos adequados e o uso de substâncias promotoras de organogênese, como as auxinas, auxiliam no processo de propagação vegetativa. Dessa maneira, objetivou-se com este trabalho avaliar o

  3. Guía ilustrada de la flora del “Desierto de la Tatacoa ”, Huila, Colombia

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    Galeano Gloria

    2004-12-01

    Full Text Available El “desierto” de La Tatacoa comprende un área de unos 335 km2, que se extienden sobre la ribera oriental del río Magdalena, en el municipio de Villavieja y parte del municipio de Baraya, en el norte del departamento del Huila; presenta una elevación promedio de 440 m, una temperatura
    promedio de 28º C y una precipitación promedio anual cercana a 1.000 mm. Históricamente,la región ha sufrido un fuerte proceso de sabanización y desertificación, por lo cual la cobertura vegetal actual se encuentra representada principalmente por matorrales bajos, rastrojos y pastizales. En este estudio se presenta el inventario florístico preliminar de la región del “desierto” de la Tatacoa; además, se caracteriza la vegetación de la región de una forma general y se presenta una guía ilustrada con descripciones botánicas para 100 de las especies más representativas de la región. Se encontraron 227 especies, distribuidas en cerca de 170 géneros y 60 familias. La gran mayoría de las especies son dicotiledóneas (85%, mientras que las monocotiledóneas se encuentran representadas solo por el 14% de las especies, y un mínimo porcentaje (menos del 1% por helechos y afines. A nivel florístico, la familia Fabaceae (s.l. es la más destacada con 35 especies y 27 géneros, representando el 15,5% del total de las especies, discriminada en las subfamilias Faboideae con 18/16, Mimosoideae con 9/7 y Caesalpinioideae con 8/4. Otras de las familias que sobresalen por su riqueza son Poaceae con 20 especies y 15 géneros, Euphorbiaceae (13/7, Asteraceae (10/10, Malvaceae (9/4, Cactaceae (8/7, Asclepiadaceae (8/7 y Convolvulaceae (8/3. Como hallazgos importantes se presenta el primer registro para el país de Senna uniflora (Caesalpiniodeae y el reencuentro de Amoreuxia palmatifida (Cochlospermaceae, especie que era conocida para Colombia por un solo espécimen coleccionado antes de 1858 en cercanías al municipio del Espinal (Tolima. La gu

  4. Biomass equations for Brazilian semiarid caatinga plants Equações para estimar a biomassa de plantas da caatinga do semi-árido brasileiro

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    Everardo V.S.B. Sampaio

    2005-12-01

    Full Text Available Allometric equations to estimate total aboveground alive biomass (B or crown projection area (C of ten caatinga species based on plant height (H and/or stem diameter at ground level (DGL or at breast height (DBH were developed. Thirty plants of each species, covering the common range of stem diameters (3 to 50 cm, were measured (C, H, DGL, DBH, cut at the base, separated into parts, weighted and subsampled to determine dry biomass. Wood density (p of the stem and the largest branches was determined. B, C, H and p ranged from 1 to 500 kg, 0.2 to 112 m², 1.3 to 11.8 m, and 0.45 to 1.03 g cm-3. Biomass of all 10 species, separately or together (excluding one cactus species, could be estimated with high coefficients of determination (R² using the power equation (B = aDGLb and DGL, DBH, H or combinations of diameter, height and density. Improvement by multiplying H and/or p to DGL or DBH was small. The mixed-species equation based only on DBH (valid up to 30 cm had a = 0.173 and b = 2.295, similar to averages of these parameters found in the literature but slightly lower than most of those for humid tropical vegetation. Crown area was significantly related to diameter, height and biomass.Equações alométricas foram desenvolvidas para estimar a biomassa aérea viva (B e a área de projeção da copa (C de dez espécies da caatinga, com base na altura da planta (H e/ou do diâmetro do caule ao nível do solo (DNS ou à altura do peito (DAP. Trinta plantas de cada espécie, cobrindo a faixa usual de diâmetros (3 a 50 cm, foram medidas (C, H, DNS, DAP, cortadas na base, separadas em partes, pesadas e subamostradas para determinação da biomassa seca. A densidade (p da madeira dos caules e galhos maiores foi determinada. B, C, H e p variaram de 1 a 500 kg, 0,2 a 112 m², 1,3 a 11,8 m e 0,45 a 1,03 g cm-3. A biomassa das 10 espécies, separadamente ou em conjunto (exceto pela espécie de Cactaceae, foi estimada com alto coeficiente de determina

  5. Tissue localization of betacyanins in cactus stems Localización de betacianinas en tejido del tallo de cactus

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    Alessandro Mosco

    2012-06-01

    Full Text Available Betalains are soluble pigments found only in the suborder Chenopodiniae, while in all other Angiospermae they are replaced by anthocyanins. The convergent evolution of the presence of anthocyanins and betalains in vegetative tissues supports the hypothesis of a similar function, based on the absorption properties of these pigments. The screening effect of anthocyanins results in the reduction of the amount of photoinhibition. betalains, being the anthocyanin counterpart in most families of Caryophyllales, were also suggested to have a screening role. This study is aimed at identifying in which Cactaceae stem tissues betacyanins, reddish to violet betalain pigments, accumulate. Stem accumulation of betacyanins was observed in cacti both in their natural habitat and in cultivation. The localization of betacyanins was assessed by light microscope studies on tubercle transverse sections. During 2 field trips in distinct years to the Mexican plateau in March, many cactus species, belonging to different genera, were observed displaying a reddish stem. Light microscope studies on cultivated plants showed that betacyanins accumulate in the hypodermis and in the outer layers of the chlorenchyma, where they may act as a screen, thus protecting the photosystems present in the underlying chlorenchyma, and have a possible antioxidant function in the cortex.Las betalaínas son pigmentos solubles que se encuentran sólo en el suborden Chenopodiniae, mientras que en el resto de Angiospermae, lo que existe son antocianinas. La evolución convergente de la presencia de antocianinas y betalaínas en tejidos vegetativos apoya la hipótesis de una función similar, que se basa en las propiedades de absorción de estos pigmentos. El efecto pantalla de las antocianinas resulta en la reducción de fotoinhibición. Siendo las betalaínas la contraparte antocianítica en la mayoría de las familias de Caryophyllales, se sugirió también un papel de pantalla de estos