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Sample records for ca tu thy

  1. Comparison of Plasma Tu-M2-PK and CA19-9 in Pancreatic Cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Heegaard, Niels H H; Schaffalitzky de Muckadell, Ove B

    2009-01-01

    because of suspicion of pancreatic cancer. Of these, 51 patients had their conditions diagnosed as PDAC, whereas this diagnosis was ruled out in 52 after 12 months of follow-up. The performance of Tu-M2-PK was compared with that of CA19-9 using cutoff values 15 and 37 U/mL, respectively. RESULTS...

  2. Thi Qar Bee Farm Thi Qar, Iraq

    Science.gov (United States)

    2010-04-01

    vegetation and fields where bees once gathered pollen and beekeepers face hardships from droughts and lack of financial assistance. 1... Beekeeping in the Fertile Crescent dates back to ancient Mesopotamia. However, beekeeping was virtually unknown in southern Iraq. In 2005, a small group of...engineers and farmers in Thi Qar province formed the Iraqi Beekeeping Association of Thi Qar and started to disseminate the culture of beekeeping

  3. Love thy nature

    National Research Council Canada - National Science Library

    Rokab, Sylvie; Souda, Elaine; Raven, Tamra; Roland, David Garfield; Chitraker, Rohan; Neeson, Liam; Rossi, Fernanda; Almeida, Eriberto

    2014-01-01

    Narrated by Liam Neeson, Love Thy Nature shows how deeply we've lost touch with nature, and takes viewers on a cinematic journey through the beauty and intimacy of our vital relationship with the natural world...

  4. Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    When honored guests visit, Tu women will block the door and invite them to show their respect by drinking three cups of wine. Only after this ritual can the guests be led indoors and shown to their seats.

  5. Structure of the murine Thy-1 gene

    NARCIS (Netherlands)

    V. Giguere; K-I. Isobe; F.G. Grosveld (Frank)

    1985-01-01

    textabstractWe have cloned the murine Thy-1.1 (AKR) and Thy-1.2 (Balb/c) genes. The complete exon/intron structure and the nucleotide sequence of the Thy-1.2 gene was determined. The gene contains four exons and three intervening sequences. The complete transcriptional unit gives rise to a tissue an

  6. Tu nos aposte protege

    DEFF Research Database (Denmark)

    Frederiksen, Britta Olrik

    2007-01-01

    Aspirationen af p, t og k i dansk er stedmoderligt behandlet af den gren af den historiske sprogforskning der bygger på skriftligt kildemateriale. Skrivemåden tu nos aposte protege for tu nos ab hoste protege 'bekyt du os mod fjenden' i en tilskrift fra anden halvdel af 1300-tallet i...

  7. The Pride of Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    The Tu ethnic group, with a population of 191,624, live mainly in Huzhu Tu Autonomous County of Qinghai Province. The Tu people, who subsist on agriculture, believe in Lamaism. Tu women are quite fond of their traditional dress with the rainbow-colored sleeves. The black stands for the earth; green, the seedlings; yellow, the mature wheat; blue, the waters and red, the Sun.

  8. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  9. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  10. Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response

    Directory of Open Access Journals (Sweden)

    Pazit Y. Cohen

    2015-01-01

    Full Text Available Lung fibrosis is characterized by abnormal accumulation of fibroblasts in the interstitium of the alveolar space. Two populations of myofibroblasts, distinguished by Thy1 expression, are detected in human and murine lungs. Accumulation of Thy1-negative (Thy1− myofibroblasts was shown in the lungs of humans with idiopathic pulmonary fibrosis (IPF and of bleomycin-treated mice. We aimed to identify genetic changes in lung myofibroblasts following Thy1 crosslinking and assess the impact of specific lung myofibroblast Thy1-deficiency, in vivo, in bleomycin-injured mouse lungs. Thy1 increased in mouse lung lymphocytes following bleomycin injury but decreased in myofibroblasts when fibrosis was at the highest point (14 days, as assessed by immunohistochemistry. Using gene chip analysis, we detected that myofibroblast Thy1 crosslinking mediates downregulation of genes promoting cell proliferation, survival, and differentiation, and reduces production of extracellular matrix (ECM components, while concurrently mediating the upregulation of genes known to foster inflammation and immunological functions. Chimeric Thy1-deficient mice with Thy1+ lymphocytes and Thy1− myofibroblasts showed fibrosis similar to wild-type mice and an increased number of CD4/CD25 regulatory T cells, with a concomitant decrease in inflammation. Lung myofibroblasts downregulate Thy1 expression to increase their proliferation but to diminish the in vivo inflammatory milieu. Inflammation is not essential for evolution of fibrosis as was previously stated.

  11. Data of evolutionary structure change: 1TU8B-2AAWC [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available n>1TU8B SIKSQ---FQFGQ > GGG--- re... C 2AAWC KNFKKEKDTPFEQ ...>HHHHHH > ATOM 2082 CA LYS C 46 3.335 27.932 37.753 1.00 ...bChain> 1TU8B LLATR----GNGRN >HGGG ----GGG ...tryIDChain> LLKKNHTNNNNDKY >HHHH

  12. Core TuLiP

    OpenAIRE

    Czenko, M.R.; Etalle, Sandro

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We present a lookup and inference algorithm which we prove to be correct and complete w.r.t. the declarative semantics. CoreTuLiP enjoys uniform syntax and the well-established semantics and is express...

  13. The Dress of Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Women of the Tu ethnicity, who live on the highlands in northwest China, wear bright and colorful dresses. The married women favor shirts with sleeves decorated in a rainbow of colored flowers: yellow, green, blue, red, purple, black

  14. ACE Inhibition in Anti-Thy1 Glomerulonephritis Limits Proteinuria but Does Not Improve Renal Function and Structural Remodeling

    Directory of Open Access Journals (Sweden)

    Peter E. Westerweel

    2012-01-01

    Full Text Available Background/Aims: ACE inhibitor (ACE-I treatment effectively inhibits proteinuria and ameliorates the course of various renal diseases. In experimental glomerulonephritis, however, angiotensin II (AngII infusion has also been shown to be renoprotective. We evaluated the long-term (28 days course of anti-Thy1 glomerulonephritis in animals with suppressed AngII formation by ACE-I treatment. Methods: Brown Norway rats received perindopril (2.8 mg/kg/day, n = 12, dihydropyridine calcium-antagonist amlodipine (Ca-A; 13 mg/kg/day, n = 6 or were left untreated (n = 14. All animals were monitored for blood pressure, proteinuria, and creatinine clearance after anti-Thy1 injection. Renal histology was assessed at day 7 and 28. Results: Systolic blood pressure was equally reduced by ACE-I and Ca-A treatment. AngII suppression prevented development of proteinuria, but did not protect against glomerular microaneurysm formation or reduction in creatinine clearance. After resolution of the microaneurysms, animals with suppressed AngII production showed a modest increase in glomerulosclerosis and vasculopathic thickening of intrarenal vessels. Conclusions: In anti-Thy1 glomerulonephritis, suppression of AngII formation does not protect against the induction of glomerular damage and is associated with mild aggravation of adverse renal fibrotic remodeling. Proteinuria, however, is effectively prevented by ACE-I treatment. Ca-A treatment did not affect the course of glomerulonephritis, indicating that ACE-I effects are blood pressure independent.

  15. [ICO-10 monoclonal antibodies to the Thy-1 antigen].

    Science.gov (United States)

    Korotkova, O V; Baryshnikov, A Iu; Tupitsyn, N N; Chimishkian, K L; Kostrykina, V N

    1989-01-01

    Mouse monoclonal antibodies (MAB) ICO-10 to Thy-1 antigen were obtained. MAB ICO-10 reacted in indirect immunofluorescence test with 5.7 +/- 0.8% human thymocytes. Antibodies did not react with granulocytes, monocytes, T- and non-T cells from peripheral blood, and with marrow cells of healthy donors. MAB ICO-10 reacted with blast cells from 25 of 53 patients with T-cell acute lymphoblastic leukemia (ALL), from 2 of 5 patients with B-cell ALL. This antigen was absent on blood and marrow cells from some patients with ALL, 80 patients with chronic lymphoid leukemia, 54 patients with chronic granulocytic leukemia at the stage of blastic crisis, 128 patients with acute nonlymphoblastic leukemia. Antibodies are specifically bound to thymocytes and spleen cells of Thy 1.1 and Thy 1.2 mice. MAB ICO-10 detect Thy-1 antigen expressed on human hematopoietic cells. MAB ICO-10 may be applied for human leukemia and lymphoma immune diagnosis.

  16. T-cell independent Thy-1 allo-antibody response with the use of transgenic mice.

    NARCIS (Netherlands)

    K-I. Isobe; G. Kollias (George); A-B. Kolsto; F.G. Grosveld (Frank)

    1986-01-01

    textabstractWe have introduced a mouse Thy-1.1 gene into the germline of Thy-1.2 mice. The introduced gene was shown to be expressed at very high levels in thymocytes when compared with the endogenous gene. Transgenic thymocytes were shown to evoke a higher than normal primary anti-Thy-1.1 antibody

  17. Structural studies of EF-Tu complexes

    DEFF Research Database (Denmark)

    Johansen, Jesper Sanderhoff

    2011-01-01

    elongation factor, EF-Ts, catalyse the dissociation of GDP from EF-Tu which in turn allows EF-Tu to bind another GTP molecule. The resulting EF-Tu:GTP complex is then cable of forming a ternary complex with a new aa-tRNA which can then associate with the ribosome. During my time as a Ph. D. student I have...... and in manuscript I. G. anatolicus is a hyperthermophilic bacteria isolated from a thermal vent. The structure of G. anatolicus EF-Ts has been determined in complex with E. coli EF-Tu by X-ray crystallography to a resolution of 2.8 Å. The structure of G. anatolicus EF-Ts is more similar to the previously determined...... structures of E. coli and bovine mitochondrial EF-Ts rather than EF-Ts from the thermophilic bacteria T. thermophilus. The structure of G. anatolicus EF-Ts in complex with E. coli EF-Tu is presented in chapter 3 and in manuscript II. Mammalian mitochondria contain an independent genome which encodes 13...

  18. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  19. Vector Loop Algebra and Its Applications to Tu Hierarchy

    Institute of Scientific and Technical Information of China (English)

    WANG Yan

    2007-01-01

    A vector loop algebra and its extended loop algebra are proposed, which are devoted to obtaining the Tu hierarchy. By making use of the extended trace identity, the Hamiltonian structure of the Tu hierarchy is constructed.Furthermore, we apply the quadratic-form identity to the integrable coupling system of the Tu hierarchy.

  20. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  1. Precursor for elongation factor Tu from Escherichia coli.

    OpenAIRE

    1986-01-01

    The tufA gene, one of two genes in Escherichia coli encoding elongation factor Tu (EF-Tu), was cloned into a ColE1-derived plasmid downstream of the lac promoter-operator. In cells carrying this plasmid, the synthesis of EF-Tu was increased four- to fivefold upon the addition of isopropyl-beta-D-thiogalactopyranoside (an inducer of the lac promoter). This condition led to the synthesis of a novel protein, called pTu, which comigrated with EF-Tu on a sodium dodecyl sulfate-polyacrylamide gel b...

  2. Artemisinin and Chinese medicine as Tu science.

    Science.gov (United States)

    Fu, Jia-Chen

    2017-07-07

    The story of discovery of artemisinin highlights the diversity of scientific values across time and space. Resituating artemisinin research within a broader temporal framework allows us to understand how Chinese drugs like qinghao came to articulate a space for scientific experimentation and innovation through its embodiment of alternating clusters of meanings associated with tu and yang within scientific discourse. Tu science, which was associated with terms like native, Chinese, local, rustic, mass, and crude, articulated a radical vision of science in the service of socialist revolutionary ideals. Yang science, which signified foreign, Western, elite, and professional, tended to bear the hallmarks of professionalism, transnational networks in education and training, and an emphasis on basic or foundational research. With respect to medical research, the case of artemisinin highlights how the constitution of socialist science as an interplay of tu and yang engendered different scientific values and parameters for scientific endeavor. Modern medical research in Maoist China could harness the productive energies of mass participation to technical expertise in its investigations of Chinese drugs, and under the banner of tu science, it became possible and scientifically legitimate to research Chinese drugs in ways that had previously provoked resistance and controversy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Thy-1 attenuates TNF-alpha-activated gene expression in mouse embryonic fibroblasts via Src family kinase.

    Directory of Open Access Journals (Sweden)

    Bin Shan

    Full Text Available Heterogeneous surface expression of Thy-1 in fibroblasts modulates inflammation and may thereby modulate injury and repair. As a paradigm, patients with idiopathic pulmonary fibrosis, a disease with pathologic features of chronic inflammation, demonstrate an absence of Thy-1 immunoreactivity within areas of fibrotic activity (fibroblast foci in contrast to the predominant Thy-1 expressing fibroblasts in the normal lung. Likewise, Thy-1 deficient mice display more severe lung fibrosis in response to an inflammatory injury than wildtype littermates. We investigated the role of Thy-1 in the response of fibroblasts to the pro-inflammatory cytokine TNF-alpha. Our study demonstrates distinct profiles of TNF-alpha-activated gene expression in Thy-1 positive (Thy-1+ and negative (Thy-1- subsets of mouse embryonic fibroblasts (MEF. TNF-alpha induced a robust activation of MMP-9, ICAM-1, and the IL-8 promoter driven reporter in Thy-1- MEFs, in contrast to only a modest increase in Thy-1+ counterparts. Consistently, ectopic expression of Thy-1 in Thy-1- MEFs significantly attenuated TNF-alpha-activated gene expression. Mechanistically, TNF-alpha activated Src family kinase (SFK only in Thy-1- MEFs. Blockade of SFK activation abrogated TNF-alpha-activated gene expression in Thy-1- MEFs, whereas restoration of SFK activation rescued the TNF-alpha response in Thy-1+ MEFs. Our findings suggest that Thy-1 down-regulates TNF-alpha-activated gene expression via interfering with SFK- and NF-kappaB-mediated transactivation. The current study provides a novel mechanistic insight to the distinct roles of fibroblast Thy-1 subsets in inflammation.

  4. Validity and reliability of the novel thyroid-specific quality of life questionnaire, ThyPRO

    DEFF Research Database (Denmark)

    Watt, Torquil; Hegedüs, Laszlo; Groenvold, Mogens;

    2010-01-01

    Background Appropriate scale validity and internal consistency reliability have recently been documented for the new thyroid-specific quality of life (QoL) patient-reported outcome (PRO) measure for benign thyroid disorders, the ThyPRO. However, before clinical use, clinical validity and test......-retest reliability should be evaluated. Aim To investigate clinical ('known-groups') validity and test-retest reliability of the Danish version of the ThyPRO. Methods For each of the 13 ThyPRO scales, we defined groups expected to have high versus low scores ('known-groups'). The clinical validity (known......-groups validity) was evaluated by whether the ThyPRO scales could detect expected differences in a cross-sectional study of 907 thyroid patients. Test-retest reliability was evaluated by intra-class correlations of two responses to the ThyPRO 2 weeks apart in a subsample of 87 stable patients. Results On all 13...

  5. Psychometric properties of the Persian version of the Tinnitus Handicap Inventory (THI-P

    Directory of Open Access Journals (Sweden)

    Mir Mohammad Jalali

    2015-03-01

    Full Text Available Introduction: Tinnitus can have a significant effect on an individual’s quality of life, and is very difficult quantify. One of the most popular questionnaires used in this area is the Tinnitus Handicap Inventory (THI. The aim of this study was to determine the reliability and validity of a Persian translation of the Tinnitus Handicap Inventory (THI-P.   Materials and Methods: This prospective clinical study was performed in the Otolaryngology Department of Guilan University of Medical Sciences, Iran. A total of 102 patients aged 23–80 years with tinnitus completed the (THI-P. The patients were instructed to complete the Beck Depression Inventory (BDI and the State-Trait Anxiety Inventory (STAI. Audiometry was performed. Eight-five patients were asked to complete the THI-P for a second time 7–10 days after the initial interview. We assessed test–retest reliability and internal reliability of the THI-P. Validity was assessed by analyzing the THI-P of patients according to their age, tinnitus duration and psychological distress (BDI and STAI. A factor analysis was computed to verify if three subscales (functional, emotional, and catastrophic represented three distinct variables.   Results: Test–retest correlation coefficient scores were highly significant. The THI-P and its subscales showed good internal consistency reliability (α = 0.80 to 0.96. High-to-moderate correlations were observed between THI-P and psychological distress and tinnitus symptom ratings. A confirmatory factor analysis failed to validate the three subscales of THI, and high inter-correlations found between the subscales question whether they represent three distinct factors. Conclusion:  The results suggest that the THI-P is a reliable and valid tool which can be used in a clinical setting to quantify the impact of tinnitus on the quality of life of Iranian patients.

  6. Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression.

    Directory of Open Access Journals (Sweden)

    Thomas E Taylor-Clark

    Full Text Available The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP in specific subsets of peripheral and central neurons. The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure and function. Here, we have characterized the expression of YFP in the sensory ganglia (DRG, trigeminal and vagal of the Thy1.2 YFP-16 mouse, using biochemical, functional and anatomical analyses. Despite previous reports, we found that YFP was only expressed in approximately half of DRG and trigeminal neurons and less than 10% of vagal neurons. YFP-expression was only found in medium and large-diameter neurons that expressed neurofilament but not TRPV1. YFP-expressing neurons failed to respond to selective agonists for TRPV1, P2X(2/3 and TRPM8 channels in Ca2+ imaging assays. Confocal analysis of glabrous skin, hairy skin of the back and ear and skeletal muscle indicated that YFP was expressed in some peripheral terminals with structures consistent with their presumed non-nociceptive nature. In summary, the Thy1.2 YFP-16 mouse expresses robust YFP expression in only a subset of sensory neurons. But this mouse model is not suitable for the study of nociceptive nerves or the function of such nerves in pain and neuropathies.

  7. Darbepoetin-α treatment enhances glomerular regenerative process in the Thy-1 glomerulonephritis model.

    Science.gov (United States)

    Cañadillas, Sagrario; Ortega, Rosa; Estepa, Jose-Carlos; Egea, Jeronimo; Gonzalez-Menchen, Alberto; Perez-Seoane, Carlos; Lopez-Andreu, Maria; Ramirez, Rafael; Tetta, Ciro; Rodriguez, Mariano; Martin-Malo, Alejandro; Aljama, Pedro

    2010-12-01

    Recent studies have demonstrated that erythropoietin (EPO) and its analogs induce cytoprotective effects on many nonerythroid cells. In this study, we examined whether darbepoetin-α might prevent glomerular lesions in the Thy-1.1 model of glomerulonephritis (Thy-1-GN). GN was induced in Wistar rats by a single injection of monoclonal anti-Thy-1.1 antibody. Rats were killed at 24 h, 72 h, 7 days, 10 days, or 15 days after antibody injection. Kidneys were removed for histological analysis, and proteinuria was measured. Because at day 7 the maximal degree of renal damage and proteinuria was found, the effect of darbepoetin-α was tested at day 7 and two different protocols of administration were used; After anti-Thy-1.1 injection, rats received two doses of darbepoetin-α or vehicle at days 0 and 4 or at days 4 and 6. At day 7, proteinuria, plasma creatinine concentration, and renal morphology analysis were performed. Also, α-actin, desmin, caspase-3, and Ki67 protein expression were evaluated by immunohistochemistry. Our results showed that in both protocols of administration, darbepoetin-α treatment decreased proteinuria in Thy-1-GN rats and this effect correlated with the improvement in renal morphology. Glomerular lesions, α-actin, and caspase-3 protein expression, observed in most glomeruli of Thy-1-GN rats, were significantly reduced in darbepoetin-α-treated rats, while cell proliferation was significantly enhanced. The results indicate that darbepoetin-α treatment promotes glomerular recovery.

  8. Structural studies of EF-Tu complexes

    DEFF Research Database (Denmark)

    Johansen, Jesper Sanderhoff

    2011-01-01

    proteins and 22 tRNAs and 2 rRNAs. The mammalian mitochondria have their own specialised translational system for maintaining the synthesis of the 13 proteins. The mammalian mitochondrial protein synthesis resembles the prokaryotic system more than the cytosolic system from eukaryotes. Some of the t...... and tRNASerGCU was produced by in vitro transcription. The ternary complex composed of EF-Tu:GDPNP:tRNASerGCU was reconstituted for crystallisation experiments. Unfortunately, we have not succeeding in crystallising neither the free components nor the ternary complex so far. The results of this project...

  9. Chloroplast EF-Tu and thermal aggregation of Rubisco activase

    Science.gov (United States)

    Chloroplast protein synthesis elongation factor, EF-Tu, has been implicated in heat tolerance in maize. The recombinant precursor of this protein, pre-EF-Tu, has been found to exhibit chaperone activity and protect heat-labile proteins, such as citrate synthase and malate dehydrogenase, from therma...

  10. Anomalous expression of Thy1 (CD90) in B-cell lymphoma cells and proliferation inhibition by anti-Thy1 antibody treatment

    Energy Technology Data Exchange (ETDEWEB)

    Ishiura, Yoshihito [Department of Biochemistry, Kochi University Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505 (Japan); CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Kotani, Norihiro, E-mail: kotani@kochi-u.ac.jp [CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Kochi System Glycobiology Center, Kochi University Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505 (Japan); Yamashita, Ryusuke [Department of Biochemistry, Kochi University Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505 (Japan); CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Yamamoto, Harumi [Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Yoshida Shimo-Adachi, Sakyo, Kyoto 606-8501 (Japan); Kozutsumi, Yasunori [CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Yoshida Shimo-Adachi, Sakyo, Kyoto 606-8501 (Japan); Honke, Koichi [Department of Biochemistry, Kochi University Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505 (Japan); CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Kochi System Glycobiology Center, Kochi University Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505 (Japan)

    2010-05-28

    The anti-CD20 monoclonal antibody (Ab) rituximab is accepted to be an effective therapeutic Ab for malignant B-cell lymphoma; however, discovery of other cell surface antigens is required for the option of antibody medicine. Considering that many tumor-associated antigens are glycans, we have searched glycoconjugates for the candidate antigens that therapeutic Abs target. To this end, we first focused on the difference in the glycogenes expression in terms of Epstein-Barr virus (EBV) infection of a Burkitt's lymphoma cell line, Akata. Using DNA array, flow cytometry and Western blotting, we found that Thy1 was highly expressed in EBV-positive Akata cells. Subsequently, Thy1 was found to be expressed in other B-cell lymphoma cell lines: BJAB, MutuI, and MutuIII, irrespective of EBV infection. Treatment of these cells with an anti-Thy1 monoclonal antibody inhibited proliferation more strongly than the therapeutic Ab rituximab. The B-cell lymphoma cell lines were classified based on the extent of the proliferation inhibition, which was not correlated with the expression level of Thy1. It is suggested that stable residence of receptor tyrosine kinases in lipid rafts sustains cell growth in B-cell lymphoma cells.

  11. Effect of acteoside on mesangial proliferation in rat anti-Thy 1 nephritis.

    Science.gov (United States)

    Hattori, T; Fujitsuka, N; Shindo, S

    1996-05-01

    We investigated whether acteoside can inhibit mesangial matrix expansion or mesangial cell proliferation in mesangioproliferative anti-Thy 1 nephritis. Untreated control rats were compared to the rats treated with acteoside either during early (day 1 to 8) or late (day 4 to 12) period after the induction of anti-Thy 1 nephritis. The result showed that acteoside and prednisolone treatments (in either early or late period) significantly reduced proteinuria, mesangial matrix expansion (the index of matrix expansion) and mesangial proliferation as determined by the number of proliferating nuclear cell antigen (PCNA)-positive cells. Acteoside also reduced glomerular macrophage infiltration and ICAM-1 expression in glomeruli of anti-Thy 1 nephritic rats. Furthermore, acteoside treatment markedly increased the activities of matrix metaloproteinases (MMP) in glomeruli. These results suggest that acteoside can inhibit mesangial cell proliferation and extracellular matrix overproduction by either inhibiting ICAM-1 expression or increasing activities of MMP.

  12. Characterization of Thi9, a novel thiamine (Vitamin B1) transporter from Schizosaccharomyces pombe.

    Science.gov (United States)

    Vogl, Christian; Klein, Cornelia M; Batke, Angelika F; Schweingruber, M Ernst; Stolz, Jürgen

    2008-03-21

    Thiamine is an essential component of the human diet and thiamine diphosphate-dependent enzymes play an important role in carbohydrate metabolism in all living cells. Although the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe can derive thiamine from biosynthesis, both are also able to take up thiamine from external sources, leading to the down-regulation of the enzymes involved in its formation. We have isolated the S. pombe thiamine transporter Thi9 by genetic complementation of mutants defective in thiamine biosynthesis and transport. Thi9 localizes to the S. pombe cell surface and works as a high-affinity proton/thiamine symporter. The uptake of thiamine was reduced in the presence of pyrithiamine, oxythiamine, amprolium, and the thiazole part of thiamine, indicating that these compounds are substrates of Thi9. In pyrithiamine-resistant mutants, a conserved glutamate residue close to the first of the 12 transmembrane domains is exchanged by a lysine and this causes aberrant localization of the protein. Thiamine uptake is significantly increased in thiamine-deficient medium and this is associated with an increase in thi9(+) mRNA and protein levels. Upon addition of thiamine, the thi9(+) mRNA becomes undetectable within minutes, whereas the Thi9 protein appears to be stable. The protein is distantly related to transporters for amino acids, gamma-aminobutyric acid and polyamines, and not to any of the known thiamine transporters. We also found that the pyridoxine transporter Bsu1 has a marked contribution to the thiamine uptake activity of S. pombe cells.

  13. Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

    Directory of Open Access Journals (Sweden)

    Xuan Zhang, Yue-feng Chu, Ping Zhao, Peng-cheng Gao, Ying He, Nu Wang and Zhong-xin Lu*

    2013-11-01

    Full Text Available Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E.coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease.

  14. Biochemical Characterization of ThiT from Lactococcus lactis : A Thiamin Transporter with Picomolar Substrate Binding Affinity

    NARCIS (Netherlands)

    Erkens, Guus B.; Slotboom, Dirk Jan

    2010-01-01

    The putative thiamin transporter ThiT from Lactococeus lactis was overproduced in the membrane of lactococcal cells. In vivo transport assays using radiolabeled thiamin demonstrated that ThiT indeed was involved in thiamin transport. The protein was solubilized from the membranes and purified in det

  15. Validity and reliability of the novel thyroid-specific quality of life questionnaire, ThyPRO

    DEFF Research Database (Denmark)

    2010-01-01

    Appropriate scale validity and internal consistency reliability have recently been documented for the new thyroid-specific quality of life (QoL) patient-reported outcome (PRO) measure for benign thyroid disorders, the ThyPRO. However, before clinical use, clinical validity and test......-retest reliability should be evaluated....

  16. ATTENUATION OF ANTI-THY1 GLOMERULONEPHRITIS IN THE RAT BY ANTIINFLAMMATORY PLATELET-INHIBITING AGENTS

    NARCIS (Netherlands)

    POELSTRA, K; BROUWER, E; BALLER, JFW; HARDONK, MJ; BAKKER, WW

    Although both ecto-ADPase and prostacyclin (PGI2) inhibit platelets and neutrophils, their action in acute glomerulonephritis is unknown. We tested the PGI2 analog Iloprost and 2chloro-adenosine (2Cl-ADO), an analog of adenosine, the end product of nucleotidase activities, during anti-Thy1

  17. Confirmatory factor analysis of the thyroid-related quality of life questionnaire ThyPRO

    DEFF Research Database (Denmark)

    Grønvold, Mogens; Deng, N; Gandek, B;

    2014-01-01

    .ResultsEach ThyPRO scale was adequately represented by a unidimensional model after minor revisions. Eleven items were identified in the unidimensional models as potentially misfitting and were investigated further by multidimensional modeling.ConclusionElaborate psychometric modeling supported the construct...

  18. TU PATERE LEGEM QUAM IPSE FECISTI

    CERN Multimedia

    Association du personnel

    2010-01-01

    In two judgments of February 2009, the ILO Administrative Tribunal (hereafter ILOAT) upheld CERN’s contract policy. In another case having given rise to a recent judgment (3 February 2010), the same problems, in essence, were raised concerning the illegal injustice which could result from the implementation of the policy in question. This time, the Tribunal decided in favour of our colleague, basing itself on the Latin maxim tu patere legem quam ipse fecisti, according to which any authority is bound by its own rules, as long as such rules have not been amended or abrogated. In fact, the Administration had informed the complainant that his application for a long-term contract would be examined for six slots, when in fact it was examined for only one. This judgment represents a victory for the complainant, for the Staff Association and its perseverance, and also for the principle according to which any organisation must keep its word and act in good faith. It shows that, without reversing its jud...

  19. EF-Tu dynamics during pre-translocation complex formation: EF-Tu·GDP exits the ribosome via two different pathways.

    Science.gov (United States)

    Liu, Wei; Chen, Chunlai; Kavaliauskas, Darius; Knudsen, Charlotte R; Goldman, Yale E; Cooperman, Barry S

    2015-10-30

    The G-protein EF-Tu, which undergoes a major conformational change when EF-Tu·GTP is converted to EF-Tu·GDP, forms part of an aminoacyl(aa)-tRNA·EF-Tu·GTP ternary complex (TC) that accelerates the binding of aa-tRNA to the ribosome during peptide elongation. Such binding, placing a portion of EF-Tu in contact with the GTPase Associated Center (GAC), is followed by GTP hydrolysis and Pi release, and results in formation of a pretranslocation (PRE) complex. Although tRNA movement through the ribosome during PRE complex formation has been extensively studied, comparatively little is known about the dynamics of EF-Tu interaction with either the ribosome or aa-tRNA. Here we examine these dynamics, utilizing ensemble and single molecule assays employing fluorescent labeled derivatives of EF-Tu, tRNA, and the ribosome to measure changes in either FRET efficiency or fluorescence intensity during PRE complex formation. Our results indicate that ribosome-bound EF-Tu separates from the GAC prior to its full separation from aa-tRNA, and suggest that EF-Tu·GDP dissociates from the ribosome by two different pathways. These pathways correspond to either reversible EF-Tu·GDP dissociation from the ribosome prior to the major conformational change in EF-Tu that follows GTP hydrolysis, or irreversible dissociation after or concomitant with this conformational change.

  20. Structure of the Mycobacterium tuberculosis Flavin Dependent Thymidylate Synthase (MtbThyX) at 2.0 Å Resolution

    Energy Technology Data Exchange (ETDEWEB)

    Sampathkumar, Parthasarathy; Turley, Stewart; Ulmer, Jonathan E.; Rhie, Ho Gun; Hopkins Sibley, Carol; Hol, Wim G.J. (Kyunghee); (UWASH)

    2010-07-20

    A novel flavin-dependent thymidylate synthase was identified recently as an essential gene in many archaebacteria and some pathogenic eubacteria. This enzyme, ThyX, is a potential antibacterial drug target, since humans and most eukaryotes lack the thyX gene and depend upon the conventional thymidylate synthase (TS) for their dTMP requirements. We have cloned and overexpressed the thyX gene (Rv2754c) from Mycobacterium tuberculosis in Escherichia coli. The M. tuberculosis ThyX (MtbThyX) enzyme complements the E. coli {chi}2913 strain that lacks its conventional TS activity. The crystal structure of the homotetrameric MtbThyX was determined in the presence of the cofactor FAD and the substrate analog, 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdUMP). In the active site, which is formed by three monomers, FAD is bound in an extended conformation with the adenosine ring in a deep pocket and BrdUMP in a closed conformation near the isoalloxazine ring. Structure-based mutational studies have revealed a critical role played by residues Lys165 and Arg168 in ThyX activity, possibly by governing access to the carbon atom to be methylated of a totally buried substrate dUMP.

  1. College via iTunes U: TU Delft deelt graag kennis

    NARCIS (Netherlands)

    Suijker, P.

    2010-01-01

    De TU Delft publiceert lezingen, colleges en informatieve films op iTunes U van Apple. De auteur beschrijft waarom de TU Delft gekozen heeft voor iTunes U en op welke manier het project tot stand is gekomen.

  2. Elongation Factor-Tu (EF-Tu) proteins structural stability and bioinformatics in ancestral gene reconstruction

    Science.gov (United States)

    Dehipawala, Sunil; Nguyen, A.; Tremberger, G.; Cheung, E.; Schneider, P.; Lieberman, D.; Holden, T.; Cheung, T.

    2013-09-01

    A paleo-experimental evolution report on elongation factor EF-Tu structural stability results has provided an opportunity to rewind the tape of life using the ancestral protein sequence reconstruction modeling approach; consistent with the book of life dogma in current biology and being an important component in the astrobiology community. Fractal dimension via the Higuchi fractal method and Shannon entropy of the DNA sequence classification could be used in a diagram that serves as a simple summary. Results from biomedical gene research provide examples on the diagram methodology. Comparisons between biomedical genes such as EEF2 (elongation factor 2 human, mouse, etc), WDR85 in epigenetics, HAR1 in human specificity, DLG1 in cognitive skill, and HLA-C in mosquito bite immunology with EF Tu DNA sequences have accounted for the reported circular dichroism thermo-stability data systematically; the results also infer a relatively less volatility geologic time period from 2 to 3 Gyr from adaptation viewpoint. Comparison to Thermotoga maritima MSB8 and Psychrobacter shows that Thermus thermophilus HB8 EF-Tu calibration sequence could be an outlier, consistent with free energy calculation by NUPACK. Diagram methodology allows computer simulation studies and HAR1 shows about 0.5% probability from chimp to human in terms of diagram location, and SNP simulation results such as amoebic meningoencephalitis NAF1 suggest correlation. Extensions to the studies of the translation and transcription elongation factor sequences in Megavirus Chiliensis, Megavirus Lba and Pandoravirus show that the studied Pandoravirus sequence could be an outlier with the highest fractal dimension and lowest entropy, as compared to chicken as a deviant in the DNMT3A DNA methylation gene sequences from zebrafish to human and to the less than one percent probability in computer simulation using the HAR1 0.5% probability as reference. The diagram methodology would be useful in ancestral gene

  3. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential sto

  4. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  5. Trust Management in P2P systems using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  6. Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis

    DEFF Research Database (Denmark)

    Villa, Elizabeth; Sengupta, Jayati; Trabuco, Leonard G.

    2009-01-01

    In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a...

  7. Functional studies of elongation factor Tu from Escherichia coli : Site-directed mutagenesis and antibiotic action

    NARCIS (Netherlands)

    Krab, Ivo Maarten

    2001-01-01

    This PhD thesis describes several studies into the structure and function of Escherichia coli Elongation Factor Tu (EF-Tu). EF-Tu plays a central role in the bacterial protein synthesis machinery as the carrier of "coded building blocks" for protein synthesis, aminoacylated tRNA (aa-tRNA). Without E

  8. Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.

    OpenAIRE

    Vander Horn, P B; Backstrom, A D; Stewart, V; Begley, T. P.

    1993-01-01

    Escherichia coli K-12 synthesizes thiamine pyrophosphate (vitamin B1) de novo. Two precursors [4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate] are coupled to form thiamine monophosphate, which is then phosphorylated to make thiamine pyrophosphate. Previous studies have identified two classes of thi mutations, clustered at 90 min on the genetic map, which result in requirements for the thiazole or the hydroxymethylpryimidine. W...

  9. Comparative analysis of the N-glycans of rat, mouse and human Thy-1. Site-specific oligosaccharide patterns of neural Thy-1, a member of the immunoglobulin superfamily.

    Science.gov (United States)

    Williams, A F; Parekh, R B; Wing, D R; Willis, A C; Barclay, A N; Dalchau, R; Fabre, J W; Dwek, R A; Rademacher, T W

    1993-08-01

    Protein structure and tissue type are known to influence glycosylation of proteins. We have previously investigated the N-glycans at each of the three glycosylation sites of the cell surface glycoprotein Thy-1 when isolated from rat brain and thymocytes. Here we report a comparative analysis of the site-specific N-glycosylation patterns from rat (Asn 23, 74, 98), mouse (Asn 23, 75, 99) and human (Asn 23, 60, 100) neural Thy-1. Despite considerable differences in amino acid sequence, the results show a remarkable conservation of the pattern of N-glycans at corresponding sites between the three species, as judged by chromatographic comparisons and glycosidase susceptibility. This is particularly marked for sites at Asn 74/75 in rat/mouse and the equivalent site at 60 in human Thy-1, as well as for sites at Asn 98/99 and 100, respectively. The sites at Asn 23 in rat/mouse also contained almost identical glycosylation patterns, but at this site human Thy-1 showed significantly different glycosylation patterns. These site glycosylation patterns are discussed in relation to the likely accessibility of the oligosaccharides for processing. It is known that within a species, the glycosylation of Thy-1 is tissue specific; therefore, this degree of conservation of glycosylation of Thy-1 expressed in the same tissue in different species is all the more striking, given the known variation between species in the amino acid sequence of Thy-1. It is therefore proposed that neural cells have a particular requirement for specific surface carbohydrates and that the Thy-1 polypeptide serves as an appropriate carrier for these structures.

  10. Measuring Thermal Stress of Dairy Cattle Based on Temperature Humidity Index (THI in Tropical Climate

    Directory of Open Access Journals (Sweden)

    Sugiono

    2016-01-01

    Full Text Available Thermal comfort for workers is very important factor to increase their performance, as well as the comfort level of dairy cattle will influence in milk productivity. The purposes of the paper is to measure the level of heat stress and then use the information to design the dairy cattle house for increasing thermal comfort. The research is started with literature review of heat stress and early survey of environment condition e.g. temperature, wind speed and relative humidity. The next step is using the information to determine the temperature humidity index (THI level for dairy cattle with maximum THI = 86 and 84 (moderate stress. The 3D CAD model and Computational Fluid Dynamic (CFD simulation are employed to looking for solution for reducing the discomfort thermal of dairy cattle. A scenario (fan air conditioning to get better condition of thermal comfort have been successfully presented with final THI index = 76 and 78 (mild stress. Finally, the paper shows how to reduce heat stress of cattle house by installation 3 exhaust fans in tropical climate.

  11. Few items in the thyroid-related quality of life instrument ThyPRO exhibited differential item functioning

    DEFF Research Database (Denmark)

    Watt, Torquil; Grønvold, Mogens; Hegedüs, Laszlo;

    2014-01-01

    To evaluate the extent of differential item functioning (DIF) within the thyroid-specific quality of life patient-reported outcome measure, ThyPRO, according to sex, age, education and thyroid diagnosis.......To evaluate the extent of differential item functioning (DIF) within the thyroid-specific quality of life patient-reported outcome measure, ThyPRO, according to sex, age, education and thyroid diagnosis....

  12. The Impending Demise of "tu" in Montevideo, Uruguay

    Science.gov (United States)

    Weyers, Joseph R.

    2009-01-01

    Since the early 1960s, the "tuteo" (T-T), and particularly the use of tonic "tu" with the "vos" verbal morphology (T-V) has been accepted as typical of the speech of Montevideo, Uruguay, a region generally classified as "voseante". This paper reports on the results of 117 rapid anonymous interviews conducted in Montevideo to document the status of…

  13. The Impending Demise of "tu" in Montevideo, Uruguay

    Science.gov (United States)

    Weyers, Joseph R.

    2009-01-01

    Since the early 1960s, the "tuteo" (T-T), and particularly the use of tonic "tu" with the "vos" verbal morphology (T-V) has been accepted as typical of the speech of Montevideo, Uruguay, a region generally classified as "voseante". This paper reports on the results of 117 rapid anonymous interviews conducted…

  14. (Average-) convexity of common pool and oligopoly TU-games

    NARCIS (Netherlands)

    Driessen, T.S.H.; Meinhardt, H.

    2000-01-01

    The paper studies both the convexity and average-convexity properties for a particular class of cooperative TU-games called common pool games. The common pool situation involves a cost function as well as a (weakly decreasing) average joint production function. Firstly, it is shown that, if the rele

  15. Problem-Centered Design and Personal Teaching Style: An Exploratory Study of Youguang Tu's Course on Philosophy of Education

    Science.gov (United States)

    Lei, Hongde

    2016-01-01

    Youguang Tu is a contemporary Chinese philosopher of education. His course on philosophy of education had a significant impact on his students. This exploratory study examines how Tu designed and taught this course. Ultimately, there are two reasons why Tu's course had such a significant influence on his students. The first is that Tu used…

  16. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants

    Directory of Open Access Journals (Sweden)

    Jianming Fu

    2012-01-01

    Full Text Available EF-Tu proteins of plastids, mitochondria, and the cytosolic counterpart EF-1α in plants, as well as EF-Tu proteins of bacteria, are highly conserved and multifunctional. The functions of EF-Tu include transporting the aminoacyl-tRNA complex to the A site of the ribosome during protein biosynthesis; chaperone activity in protecting other proteins from aggregation caused by environmental stresses, facilitating renaturation of proteins when conditions return to normal; displaying a protein disulfide isomerase activity; participating in the degradation of N-terminally blocked proteins by the proteasome; eliciting innate immunity and triggering resistance to pathogenic bacteria in plants; participating in transcription when an E. coli host is infected with phages. EF-Tu genes are upregulated by abiotic stresses in plants, and EF-Tu plays important role in stress responses. Expression of a plant EF-Tu gene confers heat tolerance in E. coli, maize knock-out EF-Tu null mutants are heat susceptible, and over-expression of an EF-Tu gene improves heat tolerance in crop plants. This review paper summarizes the current knowledge of EF-Tu proteins in stress responses in plants and progress on application of EF-Tu for developing crop varieties tolerant to abiotic stresses, such as high temperatures.

  17. Abre tu nevera! Aplicación para Android

    OpenAIRE

    Cañizares Fernandez, Rosa

    2014-01-01

    Aquí presento mi PFC Abre tu nevera! Consiste en un recetario paso a paso basado en los productos que tenemos en la despensa. Además cuenta de una gestión de listas de la compra. En el código fuente podremos encontrar una carpeta con el código fuente del proyecto Android y la app lista para instalar.

  18. Development of a Short Version of the Thyroid-Related Patient-Reported Outcome ThyPRO

    DEFF Research Database (Denmark)

    Watt, Torquil; Bjorner, Jakob Bue; Groenvold, Mogens

    2015-01-01

    BACKGROUND: Thyroid diseases affect quality of life (QoL). The Thyroid-Related Patient-Reported Outcome (ThyPRO) is an international comprehensive well-validated patient-reported outcome, measuring thyroid-related QoL. The current version is rather long-85 items. The purpose of the present study...... was to develop an abbreviated version of the ThyPRO, with conserved good measurement properties. METHODS: A cross-sectional (N = 907) and a longitudinal sample (N = 435) of thyroid patients were analyzed. A graded item response theory (IRT) model was fitted to the cross-sectional data. Short-form scales...

  19. Reconstitution of Qbeta RNA replicase from a covalently bonded elongation factor Tu-Ts complex

    DEFF Research Database (Denmark)

    Brown, S; Blumenthal, T

    1976-01-01

    (C) and Qbeta RNA normally and had approximately the same specific activity as control enzyme. Denatured Qbeta replicase formed with crosslinked EF-Tu-Ts was found to renature much more rapidly than untreated enzyme and, in contrast to normal replicase, its renaturation was not inhibited by GDP. The results...... by its ability to replace EF-Tu and EF-Ts in the renaturation of denatured Qbeta replicase. A sample of Qbeta replicase with crosslinked EF-Tu-Ts replacing the individual elongation factors was prepared. Although it lacked EF-Tu and EF-Ts activities, it could initiate transcription of both poly...

  20. Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin

    Directory of Open Access Journals (Sweden)

    Shatha F. Dallo

    2012-01-01

    Full Text Available A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs was revealed by immune electron microscopy, and its presence in the outer membrane (OM and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin.

  1. Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats

    Institute of Scientific and Technical Information of China (English)

    Saber Sakr; Laila Rashed; Waheba Zarouk; Rania El-Shamy

    2013-01-01

    Objective: To evaluate the effect of mesenchymal stem cells (MSCs) in rats with anti-Thy1,1 nephritis. Methods: Female albino rats were divided into three groups, control group, anti-Thy1,1 group and treatment with i.v. MSCs group. MSCs were derived from bone marrow of male albino rats, Y-chromosome gene was detected by polymerase chain reaction in the kidney. Serum urea and creatinine were estimated for all groups. Kidney of all studied groups was examined histologically and histochemically (total carbohydrates and total proteins). DNA fragmentation and expression of α-SMA were detected. Results:Kidney of animals injected with anti-Thy1,1 showed inflammatory leucocytic infiltration, hypertrophied glomeruli, tubular necrosis and congestion in the renal blood vessels. The kidney tissue also showed reduction of carbohydrates and total proteins together with increase in apoptosis and in expression ofα-SMA. Moreover, the levels of urea and creatinine were elevated. Treating animals with MSCs revealed that kidney tissue displayed an improvement in the histological and histochemical changes. Apoptosis and α-SMA expression were decreased, and the levels of urea and creatinine decreased. Conclusions:The obtained results demonstrated the potential of MSCs to ameliorate the structure and function of the kidney in rats with anti-Thy1,1 nephritis possibly through the release of paracrine growth factor(s).

  2. A Study on the Effects of IL-10 in Anti-Thy 1-Induced Glomerulonephritis in Rats

    NARCIS (Netherlands)

    Rachmawati, H.; Beljaars, L.; Reker-Smit, C.; Bakker, H. I.; Van Loenen-Weemaes, A. M.; Poelstra, K.

    2009-01-01

    In the present study, we examined the effects of IL-10 after 24 h in a model of acute glomerulonephritis (GN). One hour after the anti-Thy I antibody administration, a single i.v. dose of IL-10 was administered to rats. Normal rats, control nephritic rats and nephritic rats treated with IL-10, were

  3. The gammaTuRC Nanomachine Mechanism and Future Applications

    Science.gov (United States)

    Riehlman, Timothy D.

    The complexity and precision of the eukaryotic cell's cytoskeletal network is unrivaled by any man-made systems, perfected by billions of years of evolution, mastering elegant processes of self-assembly, error correction, and self-repair. Understanding the capabilities of these networks will have important and far reaching applications in human medicine by aiding our understanding of developmental processes, cellular division, and disease mechanisms, and through biomimicry will provide insights for biosynthetic manufacturing at the nanoscale and across scales. My research utilizes cross species techniques from Human to the model organism of Fission Yeast to investigate the structure and mechanisms of the g-tubulin ring complex (gTuRC). The gTuRC is a highly conserved eukaryotic multiprotein complex serving as a microtubule organizing center (MTOC) responsible for microtubule nucleation through templating, regulation of dynamics, and establishment of microtubule polarity. Microtubules are 25 nm diameter dynamic flexible polymers of a/b-tubulin heterodimers that function as scaffolds, force generators, distributors, and intracellular highways. The microtubule cytoskeleton is essential for numerous fundamental cellular processes such as mitotic division of chromosomes and cell division, organelle distribution within the cell, cell signaling, and cell shape. This incredible diversity in functions is made possible in part due to molecular motor Kinesin-like proteins (Klps), which allow expansion into more specialized neural, immune, and ciliated cell functions. Combined, the MTOC, microtubules, and Klps represent ideal microtubule cytoskeleton protein (MCP) modular components for in vitro biomimicry towards generation of adaptable patterned networks for human designed applications. My research investigates the hypothesis that a mechanistic understanding of conserved MTOC gTuRC mechanisms will help us understand dynamic cellular nanomachines and their ability to self

  4. The Measure your World / Mide tu Mundo Project

    Science.gov (United States)

    Hojman, S.; Johnson, R. M.; Meymaris, K. K.; Ward, D. L.; Russell, R.; Genyuk, J.; Lagrave, M.; Henderson, S.; Ostrosky, J.; Martinez, M.

    2007-12-01

    Over 22 centuries ago Erathostenes devised a method to determine the Earth radius. Measure your World / Mide tu Mundo is a joint venture lead by Windows to the Universe/Ventanas al Universo in the United States of America, EducaRed in Chile and RedEscolar in Mexico seeking to partner teams of students, teachers and parents from the three countries in a collaborative effort to determine the Earth circumference by sharing the results of their measurements, and exchanging cultural information. Data are collected from September 29 through October 7, 2007. A report of the whole experience will be presented.

  5. Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway.

    Science.gov (United States)

    Maldonado, H; Calderon, C; Burgos-Bravo, F; Kobler, O; Zuschratter, W; Ramirez, O; Härtel, S; Schneider, P; Quest, A F G; Herrera-Molina, R; Leyton, L

    2017-02-01

    Two key proteins for cellular communication between astrocytes and neurons are αvβ3 integrin and the receptor Thy-1. Binding of these molecules in the same (cis) or on adjacent (trans) cellular membranes induces Thy-1 clustering, triggering actin cytoskeleton remodeling. Molecular events that could explain how the Thy-1-αvβ3 integrin interaction signals have only been studied separately in different cell types, and the detailed transcellular communication and signal transduction pathways involved in neuronal cytoskeleton remodeling remain unresolved. Using biochemical and genetic approaches, single-molecule tracking, and high-resolution nanoscopy, we provide evidence that upon binding to αvβ3 integrin, Thy-1 mobility decreased while Thy-1 nanocluster size increased. This occurred concomitantly with inactivation and exclusion of the non-receptor tyrosine kinase Src from the Thy-1/C-terminal Src kinase (Csk)-binding protein (CBP)/Csk complex. The Src inactivation decreased the p190Rho GTPase activating protein phosphorylation, promoting RhoA activation, cofilin, and myosin light chain II phosphorylation and, consequently, neurite shortening. Finally, silencing the adaptor CBP demonstrated that this protein was a key transducer in the Thy-1 signaling cascade. In conclusion, these data support the hypothesis that the Thy-1-CBP-Csk-Src-RhoA-ROCK axis transmitted signals from astrocytic integrin-engaged Thy-1 (trans) to the neuronal actin cytoskeleton. Importantly, the β3 integrin in neurons (cis) was not found to be crucial for neurite shortening. This is the first study to detail the signaling pathway triggered by αvβ3, the endogenous Thy-1 ligand, highlighting the role of membrane-bound integrins as trans acting ligands in astrocyte-neuron communication.

  6. A comparison of THI indices leads to a sensible heat-based heat stress index for shaded cattle that aligns temperature and humidity stress

    Science.gov (United States)

    Berman, A.; Horovitz, Talia; Kaim, M.; Gacitua, H.

    2016-01-01

    The combined temperature-humidity heat stress is estimated in farm animals by indices derived of an index based on human thermal comfort sensation. The latter index consists of temperature and humidity measures that sum to form the temperature-humidity index (THI). The hitherto unknown relative contribution of temperature and humidity to the THI was examined. A temperature-humidity data set (temperature 20-42 °C and relative humidity 10-70 %) was used to assess by regression procedures the relative weights of temperature and humidity in the variance of THI values produced by six commonly used heat stress indices. The temperature (Ta) effect was predominant (0.82-0.95 of variance) and humidity accounted for only 0.05 to 0.12 of THI variance, half of the variance encountered in animal responses to variable humidity heat stress. Significant difference in THI values was found between indices in the relative weights of temperature and humidity. As in THI indices, temperature and humidity are expressed in different physical units, their sum has no physical attributes, and empirical evaluations assess THI relation to animal responses. A sensible heat THI was created, in which at higher temperatures humidity reaches 0.25 of sensible heat, similarly to evaporative heat loss span in heat stressed animals. It relates to ambient temperature-humidity similarly to present THI; its values are similar to other THI but greater at higher humidity. In warm conditions, mean animal responses are similar in both indices. The higher sensitivity to humidity makes this index preferable for warm-humid conditions.

  7. The Danish National Travel Survey - declaration of variables : TU 2006-13, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-13 version 2 covering data from the period May 2006 until 30. April 2014. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  8. The Danish National Travel Survey - declaration of variables : TU 2006-10, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Haunstrup, Brian

    This record documents the reported data in the data set TU 2006-10 version 1 covering data from the period May 2006 until 30 April 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  9. The Danish National Travel Survey - declaration of variables TU 2006-12, version 2 : Documentation note

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 3o April 2013. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  10. The Danish National Travel Survey - declaration of variables : TU 2006-12, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 31. December 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  11. The Danish National Travel Survey - declaration of variables TU 2006-11, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 2 covering data from the period May 2006 until 30. April 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  12. The Danish National Travel Survey - declaration of variables TU 2006-11, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 1 covering data from the period May 2006 until 31 December 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  13. Intestinal, portal, and peripheral profiles of daikenchuto (TU-100)'s active ingredients after oral administration.

    Science.gov (United States)

    Watanabe, Junko; Kaifuchi, Noriko; Kushida, Hirotaka; Matsumoto, Takashi; Fukutake, Miwako; Nishiyama, Mitsue; Yamamoto, Masahiro; Kono, Toru

    2015-10-01

    A pharmaceutical grade Japanese traditional medicine, daikenchuto (TU-100), consisting of Japanese pepper, processed ginger, and ginseng, has been widely used for various intestinal disorders in Japan and now under development as a new therapeutic drug in the US. It is suggested that TU-100 ingredients exert pharmacological effects on intestines via two routes, from the luminal side before absorption and the peripheral blood stream after absorption. Therefore, in order to fully understand the pharmacological actions of TU-100, it is critically important to know the intraluminal amounts and forms of ingested TU-100 ingredients. In the present study, after administrating TU-100 to rats, the concentrations of TU-100 ingredients and their conjugates in the peripheral and portal blood and ileal contents were determined by LC-MS/MS. Next, TU-100 was administered to patients with ileostomy bags, but whose small intestines are diagnosed as healthy, and the ingredients/conjugates in the ileal effluent were analyzed. The results suggest that: (1) Pepper ingredients hydroxysanshools are rapidly absorbed and enter systemic circulation, (2) Ginseng ingredients ginsenosides are transported to the colon with the least absorption, (3) Ginger ingredients gingerols are absorbed and some conjugated in the small intestine and transported via the portal vein. While only a small amount of gingerols/gingerol conjugates enter systemic circulation, considerable amounts reappear in the small intestine. Thus, the effect of TU-100 on the intestines is believed to be a composite of multiple actions by multiple compounds supplied via multiple routes.

  14. Test facility development for testing of micro-thrusters at TU-Delft

    NARCIS (Netherlands)

    Zandbergen, B.T.C.; Janssens, S.; Valente, F.; Perez-Grande, D.; Koopmans, R.

    2010-01-01

    The development of highly integrated and compact micro-satellites and the need for a higher precision of positioning and attitude control in space missions has stimulated the development of micro-propulsion systems. At TU-Delft, investigations are focusing on non-chemical, thermal propulsion. TU-Del

  15. The role of Glu259 in Escherichia coli elongation factor Tu in ternary complex formation

    DEFF Research Database (Denmark)

    Nautrup Pedersen, Gitte; Rattenborg, Thomas; Knudsen, Charlotte Rohde;

    1998-01-01

    Determination of the crystal structure of the ternary complex formed between elongation factor Tu:GTP and aminoacylated tRNA revealed three regions of interaction between elongation factor Tu and tRNA. The structure indicates that the conserved glutamic acid at position 271 in Thermus aquaticus EF......-Tu could be involved in the binding of the 3' CCA-Phe end of the aminoacylated tRNA. Therefore, the corresponding residue, Glu259, of Escherichia coli EF-Tu was mutated into alanine, aspartic acid, glutamine and tyrosine, in order to substantiate the crystallographic structural evidence and to obtain...... of interaction with tRNA, while mutation to tyrosine abolished completely the interaction with tRNA. Finally, mutation to glutamine resulted in an elongation factor Tu variant behaving like the wild type. In conclusion, the environment around the site binding the CCA-Phe end of the tRNA is very restricted...

  16. HIV-1 Pathogenesis and Therapeutic Intervention in the SCID-hu Thy/Liv Mouse: A Model for Primary HIV-1 Infection in the Human Thymus

    OpenAIRE

    Su, Lishan

    1997-01-01

    The SCID-hu Thy/Liv mouse is a model for the analysis of human thymopoiesis. It has been constructed by engrafting fragments of human fetal liver and thymus into the immunodeficient C.B-17 scid/scid (SCID) mouse. The resulting ‘Thy/Liv’ organ promotes long-term differentiation of human T cells. Given the apparently normal physiology of the SCID-hu Thy/Liv organ, it has been used to explore the pathophysiologic mechanisms of HIV-1 infection in vivo, and to test therapeutic modalities such as a...

  17. Physical Characterization of the Binary Asteroid 66146 (1998 Tu3)

    Science.gov (United States)

    Truong, Thien-Tin; Hicks, M.; Mayes, D.; Barajas, T.; Garcia, K.

    2011-01-01

    The near-Earth asteroid 66146 (1998 TU3) was discovered on 1998 October 13 by the LINEAR NEO survey (MPEC 1998-U03). We obtained five nights of Bessel BVRI observations (2010 Aug 6,7,10,12,13 UT) and one night of Bessel R (August 8 PST) at the JPL Table Mountain Observatory (TMO) 0.6-m telescope near Wrightwood, California. These observations were obtained as part of our ongoing survey at TMO of Potentially Hazardous Asteroids (PHAs), planetary radar targets, and low delta-V near-Earth asteroids (NEOs). The object's rotationally averaged colors (B-R=1.238+/-0.011 mag; V-R=0.440+/-0.008 mag; R-I=0.275+/-0.010 mag) were found most compatible with an Sk-type spectral classification (Bus Taxonomy)/S-type (Tholen Taxonomy). This association was obtained through a comparison of our colors with the 1341 asteroid spectra in the SMASS II database (Bus & Binzel 2002). Our classification differs significantly from the Q-type taxonomy reported by Whitely (2002). Assuming a solar phase parameter g=0.15 we performed a period search using standard Fourier techniques. We found a best-fit rotational period Psyn=2.378+/-0.001 hr, in excellent agreement with the 2.3779+/-0.0004 period determined by Richards et al. (2007). The dispersion in the phased single period lightcurve strongly suggests that 1998 TU3 is be a binary system, with variations in observed flux caused by an unresolved, tidally locked secondary companion. Fitting a 2-period model as described by Pravec et al. (2000), we found that our photometry agrees well with a binary model (P1=2.378+/-0.01 hr, P2=28.28+/-0.05 hr). We have three additional nights scheduled for this object at TMO (Oct 8, 9, 10 2010 UT), extending our solar phase coverage and allowing us to refine our rotational models. 1998 TU3 will experience an exceptional apparition in 2012. This object may be a good candidate for shape/pole modeling via lightcurve inversion, especially if photometry can be obtained from both northern and southern hemispheres. We

  18. [Management of sigmoid volvulus in the tropical area of Thies (Senegal)].

    Science.gov (United States)

    Ba, P A; Diop, B; Soumah, S A

    2015-01-01

    The aim of this study is to report our experience in the management of sigmoid volvulus in a tropical environment. This retrospective study covers the period from June 1, 2010, to December 31, 2013, in the department of emergency surgery at the regional hospital of Thiés: 40 patients were admitted with sigmoid volvulus: 36 men and 4 women, with a mean age of 45 years (20-89 years). Twelve had chronic constipation. All four signs of occlusion were present in 72.5% of cases. Abdominal radiography confirmed the diagnosis in all cases. The volvulus resolved spontaneously in one patient, while the other 39 required laparotomy: 25 had a one-stage colectomy, and 11 patients a sigmoidectomy with a temporary colostomy. Two patients underwent a sigmoidopexy, one with a simple closure of a perforated duodenal ulcer. The morbidity rate was 7.5%, due mainly to complications of infection. The mortality rate was 10%. After a mean follow-up of 2.77 months (range: 15 days-12 months), no recurrences were observed. Sigmoid volvulus is a common condition in Africa especially in younger patients. The diagnosis is easy, based on clinical and abdominal radiography findings. Several procedures have been described but the one-stage colectomy remains the method of choice, especially in tropical areas where socioeconomic conditions are difficult.

  19. Status og forvaltning af Ensianblåfugl i Nationalpark Thy

    DEFF Research Database (Denmark)

    Kelager, Andreas; Bruun, Hans Henrik; Nash, David Richard

    som igen mindsker deres levedygtighed. Disse spredningsbarrierer udgøres især af klitplantager, men sekundært også fragmentering af landskabet samt den egentlige fysiske afstand mellem populationerne. Store dele af nationalparken rummer dog potentielt egnede habitater i form af klitheder og hedemoser...... afkom. Ensianblåfugl forekommer altså kun hvor der er overlap mellem de to værter. I Danmark er de typiske habitater fugtige klitheder og hedemoser og den anses i dag som truet (VU, IUCNs rødlistestatus), da den er gået kraftigt tilbage i både populationsareal og -antal over de sidste 100 år. De primære...... årsager til nedgangen har været de massive ændringer i landanvendelsen og dræning. Forringelse af kvaliteten i de tilbageværende habitater (især tilgroning), ligger til grund for tilbagegangen i nyere tid. Thy-området er for både Ensianblåfugl og Klokke-Ensian den region i Danmark, hvor bevarelsen af...

  20. Warriors for Civilisation: Naguib Mahfouz, Andrei Tarkovsky, Tu Weiming and their Western Counterparts

    OpenAIRE

    Keir, Jonathan

    2014-01-01

    This thesis covers the decline and fall of the civilisation narrative in 20th Century Western humanities before exploring the work of three non-Western figures - Egyptian novelist Naguib Mahfouz, Russian film director Andrei Tarkovsky and Chinese philosopher Tu Weiming - who defend the concept of civilisation in their work. The views of Mahfouz, Tarkovsky and Tu can be viewed as consistent with each other and with those of contemporary Western intellectuals including John Armstrong, Martha Nu...

  1. A mechanism-based potent sirtuin inhibitor containing Nε-thiocarbamoyl-lysine (TuAcK)

    OpenAIRE

    2011-01-01

    In the current study, we have identified Nε-thiocarbamoyl-lysine (TuAcK) as a general sirtuin inhibitory warhead which was shown to be able to confer potent sirtuin inhibition. This inhibition was also shown to be mechanism-based in that the TuAck residue was able to be processed by a sirtuin enzyme with the formation of a stalled S-alkylamidate intermediate.

  2. Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Stephanie O. Palmer

    2013-01-01

    Full Text Available We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interaction of EF-Tu with GDP in the absence of EF-Ts were observed to be = 33 μM, = 0.003 s−1, and the specificity constant was  s−1 μM−1. In the presence of EF-Ts, these values were shifted to = 2 μM, = 0.005 s−1, and the specificity constant was  s−1 μM−1. The equilibrium dissociation constants governing the binding of EF-Tu to GDP ( were 30–75 nM and to GTP ( were 125–200 nM. EF-Ts stimulated the exchange of GDP by EF-Tu 10-fold. P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U-dependent binding of Phe-tRNAPhe at the A-site of P. aeruginosa ribosomes. P. aeruginosa EF-Tu was active in poly(U-programmed polyphenylalanine protein synthesis system composed of all P. aeruginosa components.

  3. An Exploration of Grid Computing to be Utilized in Teaching and Research at TU

    Directory of Open Access Journals (Sweden)

    Dr. Mohammed

    2014-11-01

    Full Text Available Taiz University (TU has a hundreds of computing resources on different campuses for use in areas from offices work to general access student labs. However, these resources are not used to their full potential. Grid computing is a technology that is capable to unify these resources and utilize them in very significant way. The difficulties of funding a complete grid computing environment and also, the difficulties of grid tools makes teachers and researchers in TU unable to involve in teaching and research in grid computing or in distributed computing. These problems raised up our awareness to mitigate this problem by build a simple environment for Grid computing from resources are available in TU and the built environment we can use it for teaching and research. The objective of this paper is to build, implement and testing a grid computing environment (Globus Toolkit. To achieving this objective we built the hardware and software parts, and configured several basic grid services commands line and web portal. The test result for basic grid services have been indicated that our proposed grid computing model is promising and can use in teaching and research in TU. The paper takes a look at how grid computing is realizing this aim and have created unbelievable opportunities for students, teachers and researchers at TU in addition the result of this paper will make TU a pilot to the other universities in whole Yemen in field of Grid and distributing computing.

  4. The multi-component Tu hierarchy of soliton equations and its multi-component integrable couplings system

    Institute of Scientific and Technical Information of China (English)

    Xia Tie-Cheng; Wang Hong; Zhang Yu-Feng

    2005-01-01

    A new simple loop algebra GM is constructed, which is devoted to the establishing of an isospectral problem. By making use of the Tu scheme, the multi-component Tu hierarchy is obtained. Furthermore, an expanding loop algebra FM of the loop algebra GM is presented. Based on the FM, the multi-component integrable coupling system of the multi-component Tu hierarchy has been worked out. The method can be applied to other nonlinear evolution equation hierarchies.

  5. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Directory of Open Access Journals (Sweden)

    Cheryl A Stoddart

    Full Text Available BACKGROUND: The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. METHODOLOGY/PRINCIPAL FINDINGS: Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3 as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. CONCLUSION: Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  6. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Science.gov (United States)

    Stoddart, Cheryl A; Bales, Cheryl A; Bare, Jennifer C; Chkhenkeli, George; Galkina, Sofiya A; Kinkade, April N; Moreno, Mary E; Rivera, José M; Ronquillo, Rollie E; Sloan, Barbara; Black, Paul L

    2007-08-01

    The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3) as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  7. THE BIOGRAPHY OF JÁNOS NAGYVÁTHY - THE FIRST HUNGARIAN AGRICULTURAL WRITER

    Directory of Open Access Journals (Sweden)

    Gábor LUKÁCS

    2002-03-01

    Full Text Available 210 years ago Nagyváthy János published the first systematic, agricultural trade book, called ’Diligent Farmers’ in Hungarian in Pest. This outstanding person of our agricultural history was born on 19th January 1755, in Miskolc. After leaving the Protestant secondary grammar school in Miskolc, he studied law and theology in Sárospatak. At the Protestant secondary grammar school in Losonc he became a ’Subrector’, that is, the the teacher of poetry. Due to a serious illness affecting his eyes he left this job and went to Pest for medical treatment. During the long therapy he visited the lectures of Mitterpacher Lajos, who was the most excellent theoratical agricultural specialist in the country. In 1782 he took military service for four years, in the Duka cavalry regiment, where he reached the rank of a captain and he was garrisoned in Belgium, Italy and Austria. As a commisariat officer he got to know the more developed agriculture of Europe, and it was at that time that he studied agricultural technical literature. In 1786 he had a relapse of his eye disease therefore he left the army and tried to find a cure in Vienna. Here he got acquainted with Jordán Péter, director of the domains of the court, who employed him as an assistant. During the time he spent in the domain of Vöslau, he could completed his academic knowledge with practical experience. In 1788 he moved to Pest where he made friends with Kazinczy, Batsányi, Verseghy and Széchenyi Ferenc, who were the representatives of the most progressive group of nobility. He became the member of the ’Magnanamitas’ Masonic Lodge. In 179o he published four pamphlets anonymously called ’ The change of religion’, ’Civil prediction from the stars’, ’The red friend’, ’Hours of joy in the life of a true Hungarian patriot’. In 1791 he published ’The diligent farmer’ in two volumes on 1213 pages recommending it to the ’sweaty Hungarian farmer’. In this book he wrote about all his

  8. [ICO-10 (Thy-1) and K-20 (gp 120/200) monoclonal antibodies in immunophenotyping of solid tumors in man].

    Science.gov (United States)

    Kadagidze, Z G; Tupitsyn, N N; Kadyrov, Kh P; Baryshnikov, A Iu; Mentkevich, G L; Matiakin, G G; Blinov, V M

    1990-01-01

    ICO - 10 (Thy-1 antigen) and K 20 (gp 120/200) monoclonal antibodies proved suitable for immunophenotyping solid tumors. The following typical antigen combinations were identified with regard to reaction with those antibodies: ICO - 10+ K 20- (neuroblastoma, malignant schwannoma, neurosarcoma and soft tissue sarcomas); K 20+ ICO - 10- (cancer of the tongue and esophagus, adenoma of the adrenal cortex, adenocarcinoma of the stomach, hepatoblastoma and nephroblastoma); ICO - 10+ K 20+ (immature teratoma and cervical and esophageal leiomyoma) and ICO - 10+ K 20- (malignant fibrous histiocytoma).

  9. ¿Arqui tec tu ra para quién? ¿Arquitectura para qué?

    Directory of Open Access Journals (Sweden)

    Carlos Vera Guardia

    2004-01-01

    Full Text Available En este dis cur so el au tor pre sen ta una vi sión crítica, filosófica, ética y epistémica de la Arquitec - tura entendida como una disciplina cientí fica y hu - manís tica. En tal sentido Arquitectura es sinónimo de: i representación sim bólica, conceptual y estéti - ca de la rea li dad, ii de sa rro llo de las ba ses ma te ria - les de la vida en su con di ción de há bi tat, y iii com - pro mi so éti co con la de fen sa y pro tec ción de las in - teracciones bióticas y ecológicas entre la naturaleza y el pro gre so hu ma no. ¿Pa ra quién y para qué?, son dos “pre gun tas para pen sar”, acer ca de los fi nes y los me dios que con vier ten a la Arqui tec tu ra en un sa ber in ter pre tar y cons truir el há bi tat de la vida hu - mana desde la perspectiva histó rica, social, am - bien tal, eco nó mi ca y po lí ti ca en la que los se res hu - manos se desenvuelven.

  10. A neutralizing anti-TGF-beta1 antibody promotes proliferation of CD34+Thy-1+ peripheral blood progenitors and increases the number of transduced progenitors.

    Science.gov (United States)

    Imbert, A M; Bagnis, C; Galindo, R; Chabannon, C; Mannoni, P

    1998-05-01

    The subset of blood cells that expresses both CD34 and Thy1 (CD90) cell surface molecules is enriched in hematopoietic stem cell activity and can be obtained from the peripheral blood of cancer patients after mobilization by chemotherapy and granulocyte colony-stimulating factor (G-CSF). Because transforming growth factor-beta1 (TGF-beta1) is a potent inhibitor of hematopoietic progenitor proliferation and differentiation, in this study we analyzed the impact of neutralizing TGF-beta1 activity during culture and retroviral transduction of CD34+Thy1+ cells. When purified CD34+Thy1+ cells were cultured in the presence of a neutralizing antibody against TGF-beta1, the percentage of cycling cells, proliferation, and absolute number of clonogenic progenitors were increased in comparison to the cultures performed without the addition of antibody. Antibody-mediated neutralization of TGF-beta1 during retroviral transduction performed by coculture of CD34+Thy1+ cells with a MFG-S-nlsLacZ retroviral vector-producing cell line did not affect the percentage of transduced progenitors as assessed by direct X-Gal staining of colonies in clonogenic assays. However, due to the better expansion of CD34+Thy1+ cells in the presence of anti-TGF-beta1, the absolute number of transduced progenitors recovered at the end of the culture was increased.

  11. Is tissue harmonic ultrasound imaging (THI) of the prostatic urethra and rectum superior to brightness (B) mode imaging? An observer study.

    Science.gov (United States)

    Sandhu, Gurpreet K; Angyalfi, Steve; Dunscombe, Peter B; Khan, Rao F

    2014-09-01

    Quality ultrasound images are an essential part of prostate brachytherapy procedure. The authors have previously reported that tissue harmonic ultrasound images (THI) are superior to brightness (B) mode for the prostate. The objective of the current study was to compare both imaging modes for visualization of the prostatic urethra and rectum. B and THI mode transrectal ultrasound images were acquired for ten patients. The prostatic urethra and rectal wall were contoured by a radiation oncologist (RO) and five observers on randomly presented images. The contours on one patient were repeated four additional times by four observers. All the images were qualitatively scored using a five-level Likert scale. The values of the Pearson product-moment correlation coefficients showed that the observers were in close agreement with the RO. Two sample paired student t-test showed that the rectum volumes with THI were significantly smaller than B-mode, but no significant difference for urethra. Two-factor analysis of variances showed significant observer variability in defining the rectum and urethra in both imaging modes. Observer consistency of the rectum volumes, estimated by standard deviations as percentages of means was significantly improved for THI. The Likert scale based qualitative assessment supported quantitative observations. The significant improvement in image quality of the prostate (reported previously) and rectum with THI may offer better-quality treatment plans for prostate brachytherapy and potential improvement in local control. Copyright © 2014 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

  12. Differential requirement for protein tyrosine kinase Fyn in the functional activation of antigen-specific T lymphocyte clones through the TCR or Thy-1.

    Science.gov (United States)

    Lancki, D W; Qian, D; Fields, P; Gajewski, T; Fitch, F W

    1995-05-01

    The protein tyrosine kinase Fyn has been shown to be involved in signal transduction through the TCR and the glycosyl-phosphatidylinositol-linked surface molecule Thy-1 expressed on T cells. In this study, we examine the requirement for Fyn expression in signaling through the TCR or Thy-1 using a panel of Ag-specific T cell clones derived from fyn-/- mutant mice. These clones do not express normal Fyn protein, as measured by immune-complex kinase reaction using anti-Fyn Ab. Stimulation through the TCR, either by APC bearing relevant Ag or by immobilized anti-CD3 mAb, resulted in comparable levels of proliferation, lymphokine production, and cytolysis by clones from both wild-type and fyn-/- mice. In contrast, stimulation through Thy-1, using soluble (or cross-linked) anti-Thy-1 mAb, was deficient, as measured by these responses. Thus, Fyn expression is selectively required for functional activation through Thy-1 in these T cell clones.

  13. Identification and characterization of Tu88, an antigenic gene from Theileria uilenbergi.

    Science.gov (United States)

    Lu, Yizhu; Wang, Yuefeng; Li, Youquan; Gou, Huitian; Luo, Jianxun; Yin, Hong; Liu, Zhijie

    2015-06-01

    Theileria uilenbergi is a pathogen that causes ovine theileriosis. Prevention and control of theileriosis relies on its diagnosis at early stages of occurrence and requires understanding of proteins with antigenic properties from the pathogen. Despite its prevalence in China, only a few molecules with antigenic properties have been characterized from T. uilenbergi. In this study, we identified a cDNA named Tu88 by immunoscreening a T. uilenbergi merozoite cDNA library with T. uilenbergi-positive sera from infected sheep. Recombinant Tu88 (rTu88) expressed in bacteria reacted strongly with the positive sera of T. uilenbergi in western blot analysis indicating its potential as an antigen. Southern blot analysis showed that it is a single copy gene. Protein localization by immunostaining blood smears from an infected sheep demonstrated the presence of native Tu88 in merozoites. These findings suggest that Tu88 is a potential candidate antigen for the development of a sero-diagnostic tool.

  14. Calibration Methods for Air Coupled Antennas - COST Action TU1208

    Science.gov (United States)

    Marecos, Vânia; Solla, Mercedes; Fontul, Simona; Pajewski, Lara

    2016-04-01

    This work focuses on the comparison of different methods for calibrating air coupled antennas: Coring, Surface Reflection Method (SRM) and Common Mid-Point (CMP) through the analysis of GPR data collected in a test site with different pavement solutions. Research activities have been carried out during a Short Term Scientific Mission (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in December 2015. The use of GPR in transport infrastructures represents one of the most significant advances for obtaining continuous data along the road, with the advantage of operation at traffic speed and being a non-destructive technique. Its main application has been the evaluation of layer thickness. For the determination of layer thickness, it is necessary to know the velocity of the signal, which depends on the dielectric constant of the material, and the two-way travel time of the reflected signal that is recorded by the GPR system. The calculation of the dielectric value of the materials can be done using different approaches such as: using fixed values based on experience, laboratory determination of dielectric values, applying the SRM, performing back calculation from ground truth references such as cores and test pits, or using the CMP method. The problem with using ground truth is that it is time consuming, labour intensive and intrusive to traffic, in addition, a drill core is not necessarily representative of the whole surveyed area. Regarding the surface reflection technique, one of the problems is that it only measures the dielectric value from the layer surface and not from the whole layer. Recent works already started to address some of these challenges proposing new approaches for GPR layer thickness measurements using multiple antennas to calculate the average dielectric value of the asphalt layer, taking advantage of significant hardware improvements in GPR

  15. Large, detergent-resistant complexes containing murine antigens Thy-1 and Ly-6 and protein tyrosine kinase p56lck.

    Science.gov (United States)

    Bohuslav, J; Cinek, T; Horejsí, V

    1993-04-01

    A number of human and mouse leukocyte surface (glyco)proteins anchored in a membrane via glycosylphosphatidylinositol (GPI) moiety have been previously shown to be noncovalently associated with protein tyrosine kinases (Science 1991. 254: 1016; J. Biol. Chem. 1992. 267: 12317). Here we show that two murine antigens of this group, Thy-1 and Ly-6, implicated in the activation of the T cells, are associated with each other, with the kinase p56lck and with several of potential kinase substrates in very large, detergent-resistant complexes, the size of which is between 50 and 200 nm, as determined by ultrafiltration and gel chromatography. Experiments on simultaneous solubilization of mixed human and mouse cells rule out that the observed complexes are artifacts induced by the detergent. Complexes of similar composition and properties were obtained when either detergents Brij-58, Nonidet-P40 or 3-[(3-cholamidopropyl)-dimethylammonio]- 1-propane-sulfonate (Chaps) were used for solubilization of the cells, while octylglucoside at least partially dissociated them. These "GPI-complexes" may be essential for the well-known signal-transducing capacity of Thy-1 and Ly-6.

  16. 小猪和小虎——SUZUKI TU250 Volty VS Kawasaki Estrella

    Institute of Scientific and Technical Information of China (English)

    杜启弘; 徐天翔

    2005-01-01

    在日本250毫升复古车中.四大厂家各自推出了自己的代表车型。雅马哈的SRV250.本田的GB250.铃木TU250 Volty、川崎Estrella。其中,GB250大获成功,SRV250则表现平平,TU250 Volty和Estrella则紧紧咬住GB250.在日本国内和国外市场上均取得不错的销量。本期试车我们就将对铃木TU250和川崎的Estrella进行对比测试。

  17. [Analysis on intestinal disorders in Jiujing Tu (Illustration of Moxiustion) found from Dunhuang].

    Science.gov (United States)

    Shi, Yin; Shang, Haixia; Wu, Huangan

    2016-05-01

    Jiujing Tu (Illustration of Moxibustion), excavated from Mo Kao Grotto at Dunhuang, is one of the earliest existing monographs on moxibustion. The medical masters from different schools have focused on this book because it is different from the existing ancient medical works and have not been collected in the medical works of different dynasties. In this study, the literature of Jiujing Tu on five acupoints (Dachangshu, Pangguangshu, Daxiaochangshu, Nieshu and Cigong) relevant with intestinal disorders is collected. It is intended to discuss and analyze the acupoint location, main intestinal disorder, moxibustion characters, recognition on the literature of different dynasties and modern clinical applications. It is believed that the thought of strong moxibustion in the treatment of intestinal disorders advocated in Jiujing Tu has profound impact on the medical development in later generations. It deserves us to have a further digging, collection and promotion of this thought in the modern time.

  18. The tuf3 gene of Streptomyces coelicolor A3(2) encodes an inessential elongation factor Tu that is apparently subject to positive stringent control

    NARCIS (Netherlands)

    Wezel, Gilles P. van; Takano, Eriko; Vijgenboom, Erik; Bosch, Leendert; Bibb, Mervyn J.

    1995-01-01

    In Streptomyces coelicolor A3(2), two genes, tuf1 and tuf3, encode the apparent polypeptide chain elongation factors EF-Tu1 and EF-Tu3, respectively. While tuf1 appears to code for the major EF-Tu, the function of tuf3 is unknown. To assess the role of EF-Tu3, tuf3 was subjected to mutational and

  19. The tuf3 gene of Streptomyces coelicolor A3(2) encodes an inessential elongation factor Tu that is apparently subject to positive stringent control

    NARCIS (Netherlands)

    Wezel, Gilles P. van; Takano, Eriko; Vijgenboom, Erik; Bosch, Leendert; Bibb, Mervyn J.

    1995-01-01

    In Streptomyces coelicolor A3(2), two genes, tuf1 and tuf3, encode the apparent polypeptide chain elongation factors EF-Tu1 and EF-Tu3, respectively. While tuf1 appears to code for the major EF-Tu, the function of tuf3 is unknown. To assess the role of EF-Tu3, tuf3 was subjected to mutational and tr

  20. Towards an understanding of structure-function relationships of elongation factor Tu

    DEFF Research Database (Denmark)

    Wiborg, O; Andersen, C; Knudsen, Charlotte Rohde

    1994-01-01

    In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point-mutated. Prel......In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point...

  1. A Practical Adaptive TuCM Scheme for Rayleigh Flat Fading Channels

    Institute of Scientific and Technical Information of China (English)

    伍守豪; 宋文涛

    2004-01-01

    A practical adaptive turbo coded modulation (TuCM) scheme was proposed and its adaptive method was described. With some hardware considerations, a suboptimal optimization algorithm that the number of fading regions is variable was put forward. Furthermore, the cutoff fade depth of power adaptation was modified to reduce the interruption probability. The results show that the proposed adaptive TuCM comes within 3 dB of Rayleigh fading channel capacity, and exhibits about 3 dB power gain relative to the conventional adaptive trellis-coded modulation (TCM), and is easy to realize by hardware.

  2. EF-Tu from the enacyloxin producing Frateuria W-315 strain: Structure/activity relationship and antibiotic resistance.

    Science.gov (United States)

    Créchet, Jean-Bernard; Malosse, Christian; Hountondji, Codjo

    2016-08-01

    In this report, we have demonstrated that the poly(U)-dependent poly(Phe) synthesis activity of elongator factor Tu (EF-Tu) from the enacyloxin producing strain Frateuria sp. W-315 is inhibited by the antibiotic similarly to that of Escherichia coli EF-Tu. The inhibitory effect of enacyloxin observed in a purified system was the same as that obtained with an S30 extract from E. coli or Frateuria sp. W-315, respectively, suggesting that antibiotic resistance of enacyloxin producing Frateuria sp. W-315 is not due neither to EF-Tu nor to other components of the translation machinery but to a still unknown mechanism. The EF-Tu gene, as PCR amplified from Frateuria W-315 genomic DNA and sequenced represented an ORF of 1191 nucleotides corresponding to 396 amino acids. This protein is larger than the product of tufA from E. coli by only two amino acid residues. Alignment of the amino acid sequence of EF-Tu from E. coli with those of Frateuria and Ralstonia solanacearum indicates on average 80% identical amino acid residues and 9.7% conservative replacements between EF-Tu Frateuria and EF-Tu E. coli, on one hand, and 97% identity and 1.7% conservative replacement between EF-Tu Frateuria and EF-Tu Ralstonia solanacearum, on the other hand. These strong primary structure similarities between EF-Tu from different origins are consistent with the fact that this factor is essential for the translation process in all kingdoms of life. Comparison of the effects of antibiotics on EF-Tu Frateuria and EF-Tu E. coli revealed that enacyloxin, kirromycin and pulvomycin exert a stronger stimulation of the GDP dissociation rate on EF-Tu Frateuria, while the effects of the antibiotics on the GDP association rate were comparable for the two EF-Tu species. Different mutants of EF-Tu E. coli were constructed with the help of site directed mutagenesis by changing one or several residues of EF-Tu E. coli by the corresponding residues of EF-Tu Frateuria. The single A45K substitution did

  3. "V tu ossen mõ ne nabljudali tshissel..." : [luuletused] / Svetlana Blomberg

    Index Scriptorium Estoniae

    Blomberg, Svetlana

    2000-01-01

    Sisu: "V tu ossen mõ ne nabljudali tshissel..." ; "Tshto delat mne, zalozhnitse zimõ..." ; "Zlaja tshornaja burja na mir naletela..." ; Stirka na beregu zaliva ; Mozhno s proshlõm sporit i srazhatsja ; Mezhdu listjev opavshih kashtan otõskat..."

  4. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    NARCIS (Netherlands)

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential

  5. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.

    Science.gov (United States)

    Jonák, J

    2007-04-15

    Prokaryotic elongation factors EF-Tu form a family of homologous, three-domain molecular switches catalyzing the binding of aminoacyl-tRNAs to ribosomes during the process of mRNA translation. They are GTP-binding proteins, or GTPases. Binding of GTP or GDP regulates their conformation and thus their activity. Because of their particular structure and regulation, various activities (also outside of the translation system) and a relative abundance they represent attractive tools for studies of many basic but still not fully understood mechanisms both of the translation process, the structure-function relationships in EF-Tu molecules themselves and proteins and energy transduction mechanisms in general. The review critically summarizes procedures for the isolation and purification of native and engineered eubacterial elongation factors EF-Tu and their mutants on a large as well as small scale. Current protocols for the purification of both native and polyHis-tagged or glutathione-S-transferase (GST)-tagged EF-Tu proteins and their variants using conventional procedures and the Ni-NTA-Agarose or Glutathione Sepharose are presented.

  6. An efficient algorithm for nucleolus and prekernel computation in some classes of TU-games

    NARCIS (Netherlands)

    Faigle, U.; Kern, W.; Kuipers, J.

    1998-01-01

    We consider classes of TU-games. We show that we can efficiently compute an allocation in the intersection of the prekernel and the least core of the game if we can efficiently compute the minimum excess for any given allocation. In the case where the prekernel of the game contains exactly one core

  7. CWI and TU Delft at TREC 2013: Contextual Suggestion, Federated Web Search, KBA, and Web Tracks

    NARCIS (Netherlands)

    Bellogín Kouki, A.; Gebremeskel, G.G.; He, J.; Lin, J.J.P.; Said, A.; Samar, T.; Vries, A.P. de; Vuurens, J.B.P.

    2014-01-01

    This paper provides an overview of the work done at the Centrum Wiskunde & Informatica (CWI) and Delft University of Technology (TU Delft) for different tracks of TREC 2013. We participated in the Contextual Suggestion Track, the Federated Web Search Track, the Knowledge Base Acceleration (KBA) Trac

  8. RESULTS OF AIRPLANE TU-204-300 OPERATED BY "VLADIVOSTOK AVIA" COMPANY FUEL FLOW MONITIRING

    Directory of Open Access Journals (Sweden)

    G. E. Maslennikova

    2014-01-01

    Full Text Available This article describes the results obtained from continuous monitoring of fuel flow on the airplanes Tu-204-300, operated by the aircompany "Vladivostok Avia". The reasons for the change in the cost of each copy of airplane and changes in fuel characteristics due to pilots manner of flying.

  9. XIA AND THE TU STATE IN THE ORACLE INSCRIPTIONS: A CONSIDERATION

    Institute of Scientific and Technical Information of China (English)

    HuHouxuan

    1995-01-01

    In recent years,the studies on the Xia culture entered a new stage~2.In thepresent article,the writer investigates the identification of the Xia peoplewith the Tu state based on the archaeological evidence,the ancient recordsand the oracle inscriptions of the Shang Dynasty.

  10. HOPE: Helmet Optimization in Europe. The final report of COST Action TU1101.

    NARCIS (Netherlands)

    Bogerd, C.P. Annaheim, S. Halldin, P. Houtenbos, M. Otte, D. Shinar, D. Walker, I. & Willinger, R.

    2015-01-01

    The primary purpose of COST Action TU1101 was to stimulate collaboration and networking amongst European scientists working in the field of bicycle helmet safety and improvement. By gathering together in a single, collective Action, researchers can improve the collection and dissemination of data fr

  11. HOPE: Helmet Optimization in Europe. The final report of COST Action TU1101.

    NARCIS (Netherlands)

    Bogerd, C.P. Annaheim, S. Halldin, P. Houtenbos, M. Otte, D. Shinar, D. Walker, I. & Willinger, R.

    2015-01-01

    The primary purpose of COST Action TU1101 was to stimulate collaboration and networking amongst European scientists working in the field of bicycle helmet safety and improvement. By gathering together in a single, collective Action, researchers can improve the collection and dissemination of data fr

  12. Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA

    DEFF Research Database (Denmark)

    Wiborg, Ove; Andersen, C; Knudsen, Charlotte Rohde

    1996-01-01

    were characterized with respect to thermal and chemical stability, GTPase activity, tRNA affinity, and activity in an in vitro translation assay. Most conspicuously tRNA affinities were reduced for all mutants. The results verify our structural analysis of elongation factor Tu in complex with aminoacyl...

  13. Ar fotosintēzi saistīto parametru sezonālās izmaiņas dažādām boreonemorālo briofītu sugām atkarībā no ūdens satura

    OpenAIRE

    Gaile, Laura

    2014-01-01

    Darbā aprakstītas ar fotosintēzi saistīto parametru izmaiņas dažādām briofītu sugām boreonemorālajos biotopos saistībā ar ūdens saturu. Mērķis bija noteikt fotosintēzes pigmentu koncentrāciju sezonālās izmaiņas dažādām briofītu sugām no atšķirīgiem biotopiem un meklēt to saistību ar augšanas substrātu, funkcionālo piederību, ūdens saturu, kā arī, fotosintēzes fotoķīmisko reakciju intensitāti, ko raksturo hlorofila fluorescences rādītāji. Kopā ievāca 25 briofītu sugu 26 paraugus no pieciem daž...

  14. α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum.

    Science.gov (United States)

    Hu, Weiqun; Zhang, Xiaoping; Chen, Xiang; Zheng, Jingwu; Yin, Yanni; Ma, Zhonghua

    2015-04-01

    The filamentous ascomycete Fusarium asiaticum contains two homologous genes FaTUA1 and FaTUA2 encoding α-tubulins. In this study, we found that FaTUA2 was dispensable for vegetative growth and sporulation in F. asiaticum. The deletion of FaTUA1 however led to dramatically reduced mycelial growth, twisted hyphae and abnormal nuclei in apical cells of hyphae. The FaTUA1 deletion mutant (ΔFaTuA1-5) also showed a significant decrease in conidiation, and produced abnormal conidia. Pathogenicity assays showed that ΔFaTuA1-5 exhibited decreased virulence on wheat head. Unexpectedly, the deletion of FaTUA1 led to resistance to high temperatures. In addition, ΔFaTuA2 showed increased sensitivity to carbendazim. Furthermore, increased FaTUA2 expression in ΔFaTuA1-5 partially restored the defects of the mutant in mycelial growth, conidial production and virulence, vice versa, increased FaTUA1 expression in the FaTUA2 deletion mutant also partially relieved the defect of the mutant in the delay of conidial germination. Taken together, these results indicate that FaTuA1 plays crucial roles in vegetative growth and development, and the functions of FaTuA1 and FaTuA2 are partially interchangeable in F. asiaticum.

  15. I Passiuna tu Christù - Rito e teatro di una cantica popolare della Grecìa Salentina

    Directory of Open Access Journals (Sweden)

    Diana Costa

    2012-01-01

    Full Text Available Abstract – IT Questo articolo si basa sugli esiti di una ricerca di campo condotta nei paesi della Grecìa Salentina,una zona del basso Salento, dove si riscontra una peculiare modalità di esecuzione della cantica popolare grìca conosciuta come I Passiuna tu Christù (La Passione di Cristo. Attorno alla metà degli anni ´90 del ´900 la tradizione della cantica è stata oggetto di un prezioso recupero, fondato sulla memoria e disponibilità di alcuni anziani cantori, che l’ha riportata in vita dopo anni di silenzio. Lo studio si è quindi focalizzato sull'esecuzione di Zollino, uno dei comuni grecanico-salentini, dove opera l'associazione culturale "Bottega del Teatro" che per prima ha promosso il recupero di cui si è detto. Protagonista e interprete raffinato della versione zollinese è l'anziano cantore Antimo Pellegrino, la cui voce si fa teatro attraverso un uso sapiente del corpo fatto di gesti stilizzati, regolati da codici che consentono di comprendere il divenire performativo, ossia il testo in grìco, anche a chi non intende tale idioma. Abstract – EN This article is based on the results of a field research conducted in the countries of the Grecìa Salentina, an area of Salento, where there is a particular execution method of the folksong known as I Passiuna tu Christù (The Passion of the Christ. Around the mid-90s of the 20th century the tradition of this folksong has been the subject of a careful recovery, based on memory and availability of some older singers, who brought it back to life after years of silence. The study then focused on the Zollino’s execution, one of the grecanico-salentino’s common, in which works the cultural association "Bottega del Teatro", the first promoter of this recovery. The old singer Antimo Pellegrino is the player and interpreter of the Zollino’s refined version, whose voice becomes theater through a wise use of body made of stylized gestures, regulated by codes that allow you to

  16. Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation

    Science.gov (United States)

    Scotti, John S.; Leung, Ivanhoe K. H.; Ge, Wei; Bentley, Michael A.; Paps, Jordi; Kramer, Holger B.; Lee, Joongoo; Aik, WeiShen; Choi, Hwanho; Paulsen, Steinar M.; Bowman, Lesley A. H.; Loik, Nikita D.; Horita, Shoichiro; Ho, Chia-hua; Kershaw, Nadia J.; Tang, Christoph M.; Claridge, Timothy D. W.; Preston, Gail M.; McDonough, Michael A.; Schofield, Christopher J.

    2014-01-01

    The roles of 2-oxoglutarate (2OG)-dependent prolyl-hydroxylases in eukaryotes include collagen stabilization, hypoxia sensing, and translational regulation. The hypoxia-inducible factor (HIF) sensing system is conserved in animals, but not in other organisms. However, bioinformatics imply that 2OG-dependent prolyl-hydroxylases (PHDs) homologous to those acting as sensing components for the HIF system in animals occur in prokaryotes. We report cellular, biochemical, and crystallographic analyses revealing that Pseudomonas prolyl-hydroxylase domain containing protein (PPHD) contain a 2OG oxygenase related in structure and function to the animal PHDs. A Pseudomonas aeruginosa PPHD knockout mutant displays impaired growth in the presence of iron chelators and increased production of the virulence factor pyocyanin. We identify elongation factor Tu (EF-Tu) as a PPHD substrate, which undergoes prolyl-4-hydroxylation on its switch I loop. A crystal structure of PPHD reveals striking similarity to human PHD2 and a Chlamydomonas reinhardtii prolyl-4-hydroxylase. A crystal structure of PPHD complexed with intact EF-Tu reveals that major conformational changes occur in both PPHD and EF-Tu, including a >20-Å movement of the EF-Tu switch I loop. Comparison of the PPHD structures with those of HIF and collagen PHDs reveals conservation in substrate recognition despite diverse biological roles and origins. The observed changes will be useful in designing new types of 2OG oxygenase inhibitors based on various conformational states, rather than active site iron chelators, which make up most reported 2OG oxygenase inhibitors. Structurally informed phylogenetic analyses suggest that the role of prolyl-hydroxylation in human hypoxia sensing has ancient origins. PMID:25197067

  17. Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation.

    Science.gov (United States)

    Scotti, John S; Leung, Ivanhoe K H; Ge, Wei; Bentley, Michael A; Paps, Jordi; Kramer, Holger B; Lee, Joongoo; Aik, WeiShen; Choi, Hwanho; Paulsen, Steinar M; Bowman, Lesley A H; Loik, Nikita D; Horita, Shoichiro; Ho, Chia-hua; Kershaw, Nadia J; Tang, Christoph M; Claridge, Timothy D W; Preston, Gail M; McDonough, Michael A; Schofield, Christopher J

    2014-09-16

    The roles of 2-oxoglutarate (2OG)-dependent prolyl-hydroxylases in eukaryotes include collagen stabilization, hypoxia sensing, and translational regulation. The hypoxia-inducible factor (HIF) sensing system is conserved in animals, but not in other organisms. However, bioinformatics imply that 2OG-dependent prolyl-hydroxylases (PHDs) homologous to those acting as sensing components for the HIF system in animals occur in prokaryotes. We report cellular, biochemical, and crystallographic analyses revealing that Pseudomonas prolyl-hydroxylase domain containing protein (PPHD) contain a 2OG oxygenase related in structure and function to the animal PHDs. A Pseudomonas aeruginosa PPHD knockout mutant displays impaired growth in the presence of iron chelators and increased production of the virulence factor pyocyanin. We identify elongation factor Tu (EF-Tu) as a PPHD substrate, which undergoes prolyl-4-hydroxylation on its switch I loop. A crystal structure of PPHD reveals striking similarity to human PHD2 and a Chlamydomonas reinhardtii prolyl-4-hydroxylase. A crystal structure of PPHD complexed with intact EF-Tu reveals that major conformational changes occur in both PPHD and EF-Tu, including a >20-Å movement of the EF-Tu switch I loop. Comparison of the PPHD structures with those of HIF and collagen PHDs reveals conservation in substrate recognition despite diverse biological roles and origins. The observed changes will be useful in designing new types of 2OG oxygenase inhibitors based on various conformational states, rather than active site iron chelators, which make up most reported 2OG oxygenase inhibitors. Structurally informed phylogenetic analyses suggest that the role of prolyl-hydroxylation in human hypoxia sensing has ancient origins.

  18. Papel de EF-Tu en la interacción de Pseudomonas aeruginosa con los queratinocitos humanos

    OpenAIRE

    Martínez Ramos, Inmaculada

    2015-01-01

    Introducción: Pseudomonas aeruginosa es un patógeno oportunista que causa una gran variedad de infecciones, entre las que destacan las infecciones respiratorias agudas y crónicas y las infecciones de heridas por quemaduras. En un trabajo previo, usamos anticuerpos específicos para detectar la presencia del epítopo de fosforilcolina en la superficie bacteriana asociado al factor de elongación Tu (EF-Tu). Identificamos que el gen eftM (Elongation Factor Tu Modifying enzime) es el...

  19. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation

    National Research Council Canada - National Science Library

    Choudhury, Nila Roy; Nowak, Jakub S; Zuo, Juan; Rappsilber, Juri; Spoel, Steven H; Michlewski, Gracjan

    2014-01-01

    .... Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation...

  20. A neutron scattering study of the ternary complex EF-Tu.GTP-valyl-tRNAVal1A

    DEFF Research Database (Denmark)

    Österberg, R.; Elias, P.; Kjems, Jørgen

    1986-01-01

    matched by the solvents. The results indicate that EF-Tu undergoes a conformational change and contracts as a result of the complex formation, since the radius of gyration decreases by 15% from 2.82 to 2.39 nm. tRNAVal1A, on the other hand, seems to mainly retain its conformation within the complex, since......The complex formation between elongation factor Tu (EF-Tu), GTP, and valyl-tRNAVal1A has been investigated in a hepes buffer of "pH" 7.4 and 0.2 M ionic strength using the small-angle neutron scattering method at concentrations of D2O where EF-Tu (42% D2O) and tRNA (71% D2O) are successively...

  1. Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TU 67).

    Science.gov (United States)

    Herrmann, F; Griffin, J D; Sabbath, K D; Oster, W; Wernet, P; Mertelsmann, R

    1988-04-01

    Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TU 67 antibody.

  2. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    Qbeta replicase is a complex of four nonidentical subunits readily dissociable into two subcomplexes: 30 S ribosomal protein S1 and the phage-coded polypeptide (Subunits I + II) and protein synthesis elongation factors EF-Tu and EF-Ts (Subunits III + IV). The affinity of the two subcomplexes...... of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP...... as measured by sensitivity of EF-Ts activity to N-ethylmaleimide....

  3. A tRNA body with high affinity for EF-Tu hastens ribosomal incorporation of unnatural amino acids.

    Science.gov (United States)

    Ieong, Ka-Weng; Pavlov, Michael Y; Kwiatkowski, Marek; Ehrenberg, Måns; Forster, Anthony C

    2014-05-01

    There is evidence that tRNA bodies have evolved to reduce differences between aminoacyl-tRNAs in their affinity to EF-Tu. Here, we study the kinetics of incorporation of L-amino acids (AAs) Phe, Ala allyl-glycine (aG), methyl-serine (mS), and biotinyl-lysine (bK) using a tRNA(Ala)-based body (tRNA(AlaB)) with a high affinity for EF-Tu. Results are compared with previous data on the kinetics of incorporation of the same AAs using a tRNA(PheB) body with a comparatively low affinity for EF-Tu. All incorporations exhibited fast and slow phases, reflecting the equilibrium fraction of AA-tRNA in active ternary complex with EF-Tu:GTP before the incorporation reaction. Increasing the concentration of EF-Tu increased the amplitude of the fast phase and left its rate unaltered. This allowed estimation of the affinity of each AA-tRNA to EF-Tu:GTP during translation, showing about a 10-fold higher EF-Tu affinity for AA-tRNAs formed from the tRNA(AlaB) body than from the tRNA(PheB) body. At ∼1 µM EF-Tu, tRNA(AlaB) conferred considerably faster incorporation kinetics than tRNA(PheB), especially in the case of the bulky bK. In contrast, the swap to the tRNA(AlaB) body did not increase the fast phase fraction of N-methyl-Phe incorporation, suggesting that the slow incorporation of N-methyl-Phe had a different cause than low EF-Tu:GTP affinity. The total time for AA-tRNA release from EF-Tu:GDP, accommodation, and peptidyl transfer on the ribosome was similar for the tRNA(AlaB) and tRNA(PheB) bodies. We conclude that a tRNA body with high EF-Tu affinity can greatly improve incorporation of unnatural AAs in a potentially generalizable manner.

  4. Differentiation of bone marrow derived Thy-1+β2M-cells into hepatocytes induced by coculture with transgenic CFSCs

    Institute of Scientific and Technical Information of China (English)

    WANG Yunfang; NAN Xue; ZHANG Rui; LI Yanhua; YUE Wen; YAN Fang; PEI Xuetao

    2004-01-01

    Studies of transplantation in vivo indicted that bone marrow derived stem cells had a potential to differentiate into mature hepatocytes. However, there are lots of doubts and uncertainties in the influencing factors and control agents of effectively inducing stem cell differentiation in vitro, the efficiency of stem cells' differentiation into hepatocytes and differentiated cells' life-span and functional state,etc. In this study, rat bone marrow derived Thy-1+β2M- cells (BDTCs) were induced to differentiate into hepatocytes by co-culturing with CFSC/HGF feeder layers which expressed hHGF efficiently and stably. RT-PCR and immunofluorescent texts proved induced BDTCs expressed infant and adult hepatocyte specific genes. Further more, these cells displayed functions of indocyanine green (ICG) uptake, ammonium metabolism and albumin production. It was shown that growth factors together with hepatic nonparenchyma cells provided a feasible microenvironment for differentiation of bone marrow stem cells into hepatocytes. The studies not only provided a significant biological model for going deep into the mechanism of stem cell plasticity, but also offered a theoretical and technical foundation of gene and stem cell engineering-based regenerative medicine for end-stage liver diseases.

  5. Mapping Anatomy to Behavior in Thy1:18 ChR2-YFP Transgenic Mice Using Optogenetics.

    Science.gov (United States)

    Fenno, Lief E; Gunaydin, Lisa A; Deisseroth, Karl

    2015-06-01

    Linking the activity of defined neural populations with behavior is a key goal of neuroscience. In the context of controlling behavior, electrical stimulation affords researchers precision in the temporal domain with gross regional specificity, whereas pharmacology allows for more specific manipulation of cell types, but in the absence of temporal precision. The use of microbial opsins--light activated, genetically encoded ion channels and pumps--to control mammalian neurons now allows researchers to "sensitize" genetically and/or topologically defined populations of neurons to light to induce either depolarization or hyperpolarization in both a cell-type-specific and temporally precise manner not achievable with previous techniques. Here, we describe the use of transgenic mice expressing the blue-light gated cation channel Channelrhodopsin-2 (ChR2) under control of the Thy1 promoter for the purpose of linking neuronal activity to behavior through restricted delivery of light to an anatomic region of interest. The surgical procedure for implanting a fiber-optic light delivery guide into the mouse brain, the process of optically stimulating the brain in a behaving animal, and post hoc evaluation are given, along with necessary reagents and discussion of common technical problems and their solutions.

  6. Genetic polymorphism of mitochondrial DNA HVS-I and HVS-Ⅱ of Chinese Tu ethnic minority group

    Institute of Scientific and Technical Information of China (English)

    Feng Chen; Yajun Deng; Yonghui Dang; BO Zhang; Haofang Mu; Xiaoguang Yu; Lin Li; Chunxia Yan; Teng Chen

    2008-01-01

    We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903:±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.

  7. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    OpenAIRE

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential of hydrogen as an alternative fuel. Find out how Forze has been developing hydrogen powered racecars since 2007.

  8. Töötu laevamehhaanik õppis seitsme kuuga kokaks / Agne Narusk

    Index Scriptorium Estoniae

    Narusk, Agne, 1971-

    2004-01-01

    Töötu Jevgeni Solovjov õppis OÜ-s Isabella kokaks, ettevõte on üks 54 koolitajast, kellelt Tallinna tööhõiveamet teenust ostab. 2004. aastaks oli Tallinna tööhõiveametil tööotsijate koolitamiseks 7 mln krooni, millest jätkus pooleks aastaks. Lisa: Tööandjad saavad küsitluslehed

  9. Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues.

    Science.gov (United States)

    Sato, Aya; Suematsu, Takuma; Aihara, Koh-Ki; Kita, Kiyoshi; Suzuki, Tsutomu; Watanabe, Kimitsuna; Ohtsuki, Takashi; Watanabe, Yoh-Ichi

    2017-03-07

    Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegansC. elegans EF-Tu2 specifically recognizes the seryl moiety of serylated D-armless tRNAs. Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: T-armless tRNAs, D-armless tRNAs, and cloverleaf tRNAs with a short T-arm. Trichinella mt EF-Tu1 binds to all three types and EF-Tu2 binds only to D-armless Ser-tRNAs, showing an evolutionary intermediate state from canonical EF-Tu to chromadorean nematode (e.g. C. elegans) EF-Tu species. We report here that two EF-Tu species also participate in Drosophila melanogaster mitochondria. Both D. melanogaster EF-Tu1 and EF-Tu2 bound to cloverleaf and D-armless tRNAs. D. melanogaster EF-Tu1 has the ability to recognize T-armless tRNAs that do not evidently exist in D. melanogaster mitochondria, but do exist in related arthropod species. In addition, D. melanogaster EF-Tu2 preferentially bound to aa-tRNAs carrying small amino acids, but not to aa-tRNAs carrying bulky amino acids. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems. © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

  10. Designing of Ground Truth Annotated DBT-TU-JU Breast Thermogram Database towards Early Abnormality Prediction.

    Science.gov (United States)

    Bhowmik, Mrinal Kanti; Gogoi, Usha Rani; Majumdar, Gautam; Bhattacharjee, Debotosh; Datta, Dhritiman; Ghosh, Anjan Kumar

    2017-08-17

    The advancement of research in a specific area of clinical diagnosis crucially depends on the availability and quality of the radiology and other test related databases accompanied by ground truth and additional necessary medical findings. The paper describes the creation of the Department of Biotechnology-Tripura University-Jadavpur University (DBT-TU-JU) breast thermogram database. The objective of creating the DBT-TU-JU database is to provide a breast thermogram database that is annotated with the ground truth images of the suspicious regions. Along with the result of breast thermography, the database comprises of the results of other breast imaging methodologies. A standard breast thermogram acquisition protocol suite comprising of several critical factors has been designed for the collection of breast thermograms. Currently, the DBT-TU-JU database contains 1100 breast thermograms of 100 subjects. Due to the necessity of evaluating any breast abnormality detection system, this study emphasizes the generation of the ground truth images of the hotspot areas, whose presence in a breast thermogram signifies the presence of breast abnormality. With the generated ground truth images, we compared the results of six state-of-the-art image segmentation methods using five supervised evaluation metrics to identify the proficient segmentation methods for hotspot extraction. Based on the evaluation results, the Fractional-Order Darwinian particle swarm optimization, Region growing, Mean shift and Fuzzy c-means clustering are found to be more efficient in comparison to k-means clustering and Threshold based segmentation methods.

  11. Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice.

    Science.gov (United States)

    Porrero, Cesar; Rubio-Garrido, Pablo; Avendaño, Carlos; Clascá, Francisco

    2010-07-23

    Transgenic mouse lines in which a fluorescent protein is constitutively expressed under the Thy1 gene promoter have become important models in cell biology and pathology studies of specific neuronal populations. As a result of positional insertion and/or copy number effects on the transgene, the populations expressing the fluorescent protein (eYFP+) vary markedly among the different mice lines. However, identification of the eYFP+ subpopulations has remained sketchy and fragmentary even for the most widely used lines such as Thy1-eYFP-H mice (Feng, G., Mellor, R.H., Bernstein, M., Keller-Peck, C., Nguyen, Q.T., Wallace, M., Nerbonne, J.M., Lichtman and J.W., Sanes. J.R. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51). Here, we provide a comprehensive mapping of labeled cell types throughout the central nervous system in adult and postnatal (P0-P30) Thy1-eYFP-H mice. Cell type identification was based on somatodendritic morphology, axon trajectories, and, for cortical cells, retrograde labeling with Fast Blue to distinguish between subpopulations with different axonal targets. In the neocortex, eYFP+ cells are layers 5 and 6 pyramidal neurons, whose abundance and sublaminar distribution varies markedly between areas. Labeling is particularly prevalent in the corticospinal cells; as a result, the pyramidal pathway axons are conspicuously labeled down to the spinal cord. Large populations of hippocampal, subicular and amygdaloid projection neurons are eYFP+ as well. Additional eYFP+ cell groups are located in specific brainstem nuclei. Present results provide a comprehensive reference dataset for adult and developmental studies using the Thy1-eYFP-H mice strain, and show that this animal model may be particularly suitable for studies on the cell biology of corticospinal neurons.

  12. Cytotoxicity acquired by conjugation of an anti-Thy1.1 monoclonal antibody and the ribosome-inactivating protein, gelonin.

    Science.gov (United States)

    Thorpe, P E; Brown, A N; Ross, W C; Cumber, A J; Detre, S I; Edwards, D C; Davies, A J; Stirpe, F

    1981-06-01

    Gelonin, a plant protein which can powerfully reduce the protein-synthetic capacity of ribosome preparations, was covalently coupled to anti-Thy1.2 antibody. The conjugate was prepared using N-succinimidyl-3-(2-pyridyldithio)propionate which generates a disulphide linkage between the component molecules. Two conjugate fractions were obtained with Mr of 180 000 and greater than 200 000. After its linkage of the antibody, gelonin suppressed those Thy1.1-bearing T lymphocytes from AKR mice which will respond to phytohaemagglutinin and concanavalin A in tissue culture. The [3H]leucine incorporation with the T-cell mitogens was inhibited by 50% with the 180 000-Mr fraction at a concentration of 0.4 nM and with the greater than 200 000-Mr fraction of pM. Unconjugated gelonin induced comparable reductions in T-cell responsiveness but at concentrations of 30 nM. The conjugates exerted little or no effect upon B lymphocytes or T lymphocytes from CBA mice (Thy1.2 + ve). Thy1.1-expressing AKR lymphoma cell lines, AKR-A and BW5147, were found to be sensitive to the conjugates, albeit much less so than the normal T lymphocytes. The conjugates injected in vivo significantly prolonged the life of CBA mice bearing in an AKR-A lymphoma allograft. It is concluded that gelonin can, by its linkage to an antibody, be rendered cytotoxic with a potency to match or exceed those of the toxins abrin and ricin.

  13. Two-photon microscopy imaging of thy1GFP-M transgenic mice: a novel animal model to investigate brain dendritic cell subsets in vivo.

    Directory of Open Access Journals (Sweden)

    Claudia Laperchia

    Full Text Available Transgenic mice expressing fluorescent proteins in specific cell populations are widely used for in vivo brain studies with two-photon fluorescence (TPF microscopy. Mice of the thy1GFP-M line have been engineered for selective expression of green fluorescent protein (GFP in neuronal populations. Here, we report that TPF microscopy reveals, at the brain surface of these mice, also motile non-neuronal GFP+ cells. We have analyzed the behavior of these cells in vivo and characterized in brain sections their immunophenotype.With TPF imaging, motile GFP+ cells were found in the meninges, subarachnoid space and upper cortical layers. The striking feature of these cells was their ability to move across the brain parenchyma, exhibiting evident shape changes during their scanning-like motion. In brain sections, GFP+ cells were immunonegative to antigens recognizing motile cells such as migratory neuroblasts, neuronal and glial precursors, mast cells, and fibroblasts. GFP+ non-neuronal cells exhibited instead the characteristic features and immunophenotype (CD11c and major histocompatibility complex molecule class II immunopositivity of dendritic cells (DCs, and were immunonegative to the microglial marker Iba-1. GFP+ cells were also identified in lymph nodes and blood of thy1GFP-M mice, supporting their identity as DCs. Thus, TPF microscopy has here allowed the visualization for the first time of the motile behavior of brain DCs in situ. The results indicate that the thy1GFP-M mouse line provides a novel animal model for the study of subsets of these professional antigen-presenting cells in the brain. Information on brain DCs is still very limited and imaging in thy1GFP-M mice has a great potential for analyses of DC-neuron interaction in normal and pathological conditions.

  14. Research data from hydrology disciplines are safely stored and published in 3TU.Datacentrum

    Science.gov (United States)

    Prinčič, Alenka; Rombouts, Jeroen

    2013-04-01

    Good management of research data from different science disciplines such as hydrology, geodesy and climate research, is crucial for reducing the uncertainties in forecasting and predictions. These research data can be well-managed and safely stored in 3TU.Datacentrum and thus become available for future use by the scientific community. 3TU.Datacentrum (datacentrum.3tu.nl) is a portal for science- and technology research data in the Netherlands. It provides long-term archive and permanent access to research data, it provides tools for citation of research datasets and thus enables linking, sharing and reuse of research data. Versatility of disciplines and thus versatility of the data producers' needs is a challenge of the data center. 3TU.Datacentrum offers solutions - as standardized as possible yet different where required. Standardized solution is the data model: datasets, instruments, locations and time frames are all objects on their own, with own metadata and interconnected through rdf relationships. These relations are also used to generate ORE Resource Maps. The file format NetCDF, which is primarily used for sets of multidimensional arrays with metadata included, enables interoperability. For simple datasets a Fedora repository is used where datasets of any format are allowed including that of a specific instrument. Alongside the NetCDF an xml version (ncml) is stored that can be converted to other formats. For complex datasets an OPeNDAP framework is used, which allows querying within and across datasets. Some examples from hydrological research are presented. 1 - For a complex collection of simple datasets consisting of ongoing hydrological measurements from several project partners, using several instruments, on several locations, and over a long period of time, there was a need for central storage in order to share the data and enable easy combination of data sets. The measurements are not repeatable and are also valuable for multidisciplinary research

  15. The computer science institute building of TU Brunswick University. Construction of an energy-efficient university building; Das Informatikzentrum der TU Braunschweig. Realisierung eines energieeffizienten Institutsgebaeudes

    Energy Technology Data Exchange (ETDEWEB)

    Rozynski, M.; Gerder, F. [Technische Univ. Braunschweig (Germany). Inst. fuer Gebaeude- und Solartechnik

    2003-07-01

    Saving of resources will be a key issue in future building construction. A new building projected on the campus of Brunswick University will have a power supply and ventilation concept that ensures low energy consumption. The project is carried out with funds provided by the Federal Minister of Economics and Technology (BMWi) in the context of the SolarBau funding concept. Construction of the building will be followed by an extensive monitoring programme that is to ensure its perfect function. [German] Eine wesentliche Zielsetzung zukuenftigen Bauens ist der sparsame Umgang mit Ressourcen. Im Rahmen eines integralen Planungsprozesses konnte fuer den Neubau des Informatikzentrums der TU Braunschweig ein Energie- und Lueftungskonzept realisiert werden, dass auf einen niedrigen Energieverbrauch zielt. Das Projekt wird im Rahmen des Foerderkonzeptes SolarBau durch das Bundesministerium fuer Wirtschaft und Technologie (BMWi) gefoerdert. Durch das anschliessende umfangreiche Monitoringprogramm wird derzeit die Funktionsfaehigkeit dieses Konzeptes ueberprueft. (orig.)

  16. Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

    Institute of Scientific and Technical Information of China (English)

    Fen Lu; Huiqin Wang; Shanzhi Wang; Wendi Jiang; Changlin Shan; Bin Li; Jun Yang; Shiyong Zhang; Wenxian Sun

    2015-01-01

    The elongation factor Tu (EF-Tu) receptor (EFR) in cruciferous plants specifical y recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants (Oryza sativa L. ssp. japonica cv. Zhonghua 17) and cel cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa-tion in the AtEFR transgenic rice cel s and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatical y in transgenic rice plant and cel lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig-gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expression level. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Col ectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

  17. Honour Thy Neighbour

    DEFF Research Database (Denmark)

    Ottosen, Thorsten Jørgen; Vomlel, Jiri

    2010-01-01

    Whenever objects and their interaction is modelled via undirected graphs, it is often of great interest to know the cliques of the graph. For several problems the graph changes frequently over time, and we therefore seek methods for updating the information about the cliques in a dynamic fashion...... to avoid expensive recomputations. This dynamic problem was investigated by Stix, and in this paper we derive a new simple method based on the Bron-Kerbosch algorithm that compares favourably to Stix' approach. The new approach is generic in the sense that it can be used with other algorithms than just...

  18. Identification of Molecular Markers Linked to TuMV Resistant Gene in Cabbage

    Institute of Scientific and Technical Information of China (English)

    GAO Jinping; WANG Chao; LIU Ying

    2008-01-01

    A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant).Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660,were 7.7 cM and 8.38 cM apart from the TuMV resistant gene,respectively. The two RAPD fragments were converted to SCAR markers.SCAR markers were confined in germplasm.

  19. Jornada "Cómo crear tu propia asesoría fiscal"

    Directory of Open Access Journals (Sweden)

    Ana María Delgado García

    2016-02-01

    Full Text Available Los Estudios de Derecho y Ciencia Política de la Universitat Oberta de Catalunya organizaron el día 23 de septiembre de 2015 la jornada «Cómo crear
    tu propia asesoría fiscal», bajo la dirección de Ana María Delgado García (Directora del Máster Universitario de Fiscalidad de la UOC y Rafael Oliver Cuello (Abogado, asesor fiscal y profesor consultor de Fiscalidad de la UOC.

  20. Opšte vibracije na sedištu pilota kod dva tipa helikoptera

    OpenAIRE

    Živković Dejan; Basarić Gradimir; Jovelić Stojan

    2002-01-01

    U ovom radu opisano je merenje opštih vibracija na sedištu pilota kod dva tipa helikoptera (po jedna letelica) sa turbomlaznim motorima, kao i trećinsko-oktavna analiza ubrzanja opštih vibracija u sve tri ose. Iz dobijenih rezultata sledi da su nivoi ubrzanja kod prvog tipa helikoptera najveći u trećinsko-oktavnom opsegu sa centralnom frekvencijom od 16 Hz. Maksimalne vrednosti ubrzanja opštih vertikalnih vibracija izmerene su za režime lebdenja (0,977 m/s2) i horizontalnog leta pri brzini od...

  1. Ciuda tu corazón, es para toda la vida -Para Disfrutar la Música Clasica

    OpenAIRE

    2012-01-01

    Cuida tu corazón, es para toda la vida. Importantelibro de información sobre prevenciónde de las enfermedades cardiovasculares,dirigido a la comunidad en general.Hace parte de la colección de libros sobre prevención primaria: “Cuidatu corazón, vive mejor” que desde hace varios años viene publicandoel profesor, doctor Fernando Manzur Jattin, desde su grupo de investigación:“Centro de Investigaciones Biomédicas”, perteneciente a laFacultad de Medicina de la Universidad de Cartagena y Categoriza...

  2. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-09, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    , eller anden årsag. Undervejs omtales en række vigtige detaljer ved spørgeskemaet, men dette notat har ikke til formål at give en udtømmende beskrivelse af hvilke spørgsmål, der er stillet til hvem og hvornår. Her kan det være til stor nytte at besøge testspørgeskemaet på http://tu2010.dk/dev/starttest.php...

  3. Tööotsija töötuks tunnistamise ning töötu abiraha määramise ja maksmise ajutise juhendi kinnitamise kohta

    Index Scriptorium Estoniae

    1991-01-01

    Kehtib 1. aprillist 1991. Lisatud ajutine juhend: töö vahendamine tööotsijale, töötule, töötu abiraha määramine ja maksmine Lisatud: töötu abiraha arvutamine. Muudatus: RT I 1992 nr. 27 art. 362

  4. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination...

  5. Protein synthesis alongation factors EF-Tu and eEF1A: biosynthesis, functions and application in the improvement of heat tolerance in plants

    Science.gov (United States)

    Protein synthesis elongation factors EF-Tu and eEF1A (EFs) represent a group of highly conserved and abundant GTPases with an important role in transporting the aminoacyl-tRNA complex to the A site of the ribosome during elongation phase of translation. EF-Tu proteins are located in bacteria and, du...

  6. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination of th...

  7. Intracellular cytokine production by Th1/Th2 lymphocytes and monocytes of children with symptomatic transient hypogammaglobulinaemia of infancy (THI) and selective IgA deficiency (SIgAD)

    Science.gov (United States)

    Kowalczyk, D; Baran, J; Webster, A D B; Zembala, M

    2002-01-01

    Intracellular expression of several cytokines was assessed in lymphocytes and monocytes of children with transient hypogammaglobulinaemia of infancy (THI) and selective IgA deficiency (SIgAD). THI was characterized by an increased frequency of CD3+/CD4+ lymphocytes expressing tumour necrosis factor α (TNF-α), TNF-β and interleukin 10 (IL-10), while in SIgAD elevated numbers of these cells containing TNF-α and interferon γ (IFN-γ) were observed. No changes in the number of CD4+ T cells expressing IL-4 in both diseases were noted. The proportion of CD33+ monocytes containing TNF-α both in THI and SIgAD was unchanged. The secretion of IL-12 by peripheral blood mononuclear cells (PBMCs) of patients with THI and SIgAD was significantly elevated and associated with an increased frequency of IL-12 expressing monocytes in THI but not in SIgAD. IL-18 secretion was slightly, but not significantly, elevated in both diseases. Intracellular Th1 and Th2 type cytokines within CD3+/CD4+ lymphocytes were also determined in the normal blood donors that showed high or low production of IgG and IgA in vitro. In low producers of IgG an increased proportion of CD3+/CD4+ cells expressing TNF-α and IFN-γ was found, while in low IgA responders only elevated TNF-α positive CD3+/CD4+ cells were observed. These results suggest that THI and SIgAD may represent diseases with an excessive Th1 type response that is associated with an up-regulation of IL-12 secretion and, at least in THI, elevated numbers of monocytes expressing intracellular IL-12. Up-regulation of IL-12 may be the essential factor in the patomechanism(s) of these diseases as already described in common variable immunodeficiency (CVID). PMID:11966768

  8. The Thyroid-Related Quality of Life Measure ThyPRO Has Good Responsiveness and Ability to Detect Relevant Treatment Effects

    DEFF Research Database (Denmark)

    Watt, Torquil; Cramon, Per; Hegedüs, Laszlo

    2014-01-01

    BACKGROUND AND PURPOSE: Patient-reported outcomes have become important endpoints in comparative effectiveness research and in patient-centered health care. Valid patient-reported outcome measures detect and respond to clinically relevant changes. The purpose of this study was to evaluate...... this measurement instrument as a patient-reported outcome in clinical studies and in clinical management....... nontoxic goiter treated with surgery or radioactive iodine and remaining euthyroid (n = 62). Changes in QoL were evaluated in terms of effect size and compared to the changes predicted by clinical experts. The responsiveness of equivalent scales from ThyPRO and SF-36 Health Survey were compared...

  9. Upregulation of group IB secreted phospholipase A(2) and its M-type receptor in rat ANTI-THY-1 glomerulonephritis.

    Science.gov (United States)

    Beck, S; Beck, G; Ostendorf, T; Floege, J; Lambeau, G; Nevalainen, T; Radeke, H H; Gurrieri, S; Haas, U; Thorwart, B; Pfeilschifter, J; Kaszkin, M

    2006-10-01

    Treatment of rat glomerular mesangial cell (GMC) cultures with pancreatic secreted phospholipase A(2) (sPLA(2)-IB) results in an enhanced expression of sPLA(2)-IIA and COX-2, possibly via binding to its specific M-type sPLA(2) receptor. In the current study, we have investigated the expression and regulation of sPLA(2)-IB and its receptor during glomerulonephritis (GN). In vivo we used the well-established rat model of anti-Thy 1.1 GN (anti-Thy 1.1-GN) to study the expression of sPLA(2)-IB and the M-type sPLA(2) receptor by immunohistochemistry. In addition, in vitro we determined the interkeukin (IL)-1beta-regulated mRNA and protein expression in primary rat glomerular mesangial and endothelial cells as well as in rat peripheral blood leukocytes (PBLs). Shortly after induction of anti-Thy 1.1-GN, sPLA(2)-IB expression was markedly upregulated in the kidney at 6-24 h. Within glomeruli, the strongest sPLA(2)-IB protein expression was detected on infiltrated granulocytes and monocytes. However, at the same time, the M-type receptor was also markedly upregulated on resident glomerular cells. In vitro, the most prominent cytokine-stimulated secretion of sPLA(2)-IB was observed in monocytes isolated from rat PBLs. Treating glomerular endothelial cells (GECs) with cytokines elicited only weak sPLA(2)-IB expression, but treatment of these cells with exogenous sPLA(2)-IB resulted in a marked expression of the endogenous sPLA(2)-IB. Mesangial cells did not express sPLA(2)-IB at all. The M-type sPLA(2) receptor protein was markedly upregulated on cytokine-stimulated mesangial and endothelial cells as well as on lymphocytes and granulocytes. During anti-Thy 1.1 rat GN, sPLA(2)-IB and the M-type sPLA(2) receptor are induced as primary downstream genes stimulated by inflammatory cytokines. Subsequently, both sPLA(2)-IB and the M-type sPLA(2) receptor are involved in the autocrine and paracrine amplification of the inflammatory process in different resident and infiltrating

  10. Role of Mutations in Dihydrofolate Reductase DfrA (Rv2763c) and Thymidylate Synthase ThyA (Rv2764c) in Mycobacterium tuberculosis Drug Resistance

    KAUST Repository

    Koser, C. U.

    2010-09-17

    We would like to comment on a number of recent reports in this journal (6, 8, 12, 18) concerning Mycobacterium tuberculosis dihydrofolate reductase (DHFR), encoded by dfrA (Rv2763c). Around 36% of phenotypically para-aminosalicylic acid (PAS)-resistant M. tuberculosis strains harbor mutations in thyA (Rv2764c), which encodes a thymidylate synthase (20). In their effort to elucidate the remaining unknown resistance mechanism(s), Mathys et al. extended their sequence analysis to a number of additional genes, including dfrA (12). It was unclear whether the three dfrA mutations they identified in the PAS-resistant strains P-693 and P-3158 could contribute to PAS resistance on their own. Nonetheless, these findings are notable for two reasons. First, isoniazid (INH) has been shown to inhibit M. tuberculosis DHFR in vitro (1). Whether the same holds true for ethionamide, which shares a number of common resistance mechanisms with INH, was not tested (J. Blanchard, personal communication). In any case, the clinical relevance of DHFR-mediated INH resistance remains enigmatic. To date, only Ho et al. have addressed this question, but they did not identify any dfrA mutations in a screen of 127 INH-resistant clinical isolates (8). Consequently, Mathys et al. remain the first to describe mutations in this target (12). However, given that isolates with mutated DHFR are members of a cluster with baseline INH resistance, the importance of these mutations with respect to INH resistance remains unclear. Irrespective of their relevance in INH resistance, these dfrA mutations are noteworthy for a second reason. Contrary to previous wisdom, Forgacs et al. recently showed that M. tuberculosis is sensitive to the drug combination trimethoprim-sulfamethoxazole (TMP-SMX) (6, 18). DHFR is competitively inhibited by TMP, and consequently, mutations therein lead to resistance in a variety of organisms (9, 16, 19). The crystal structures of the wild-type M. tuberculosis DHFR in complex with

  11. Diasystème et spécification de traits en tu'un savi (mixtecan otomangue oriental)

    OpenAIRE

    Léonard, Jean,

    2010-01-01

    This contribution aims to focus on various descriptions and theories of the segmental inventory and distributional constraints of the phonology of Tu'un Savi (Mixtec). The Oto-Manguean languages are reputed to be among the most complex of Meso-America due to the intricacy of their surface forms. Mixtec (known as Tu'un Savi or Ñuu Savi by its speakers) is part of the Mixtecan sub-family, which includes Trique, Cuicatec and probably also Amuzgo (Longacre, 1957, 1961; Longacre & Millon, 1961), b...

  12. Tuberculate fruit gene Tu encodes a C2 H2 zinc finger protein that is required for the warty fruit phenotype in cucumber (Cucumis sativus L.).

    Science.gov (United States)

    Yang, Xuqin; Zhang, Weiwei; He, Huanle; Nie, Jingtao; Bie, Beibei; Zhao, Junlong; Ren, Guoliang; Li, Yue; Zhang, Dabing; Pan, Junsong; Cai, Run

    2014-06-01

    Cucumber fruits that have tubercules and spines (trichomes) are known to possess a warty (Wty) phenotype. In this study, the tuberculate fruit gene Tu was identified by map-based cloning, and was found to encode a transcription factor (TF) with a single C2 H2 zinc finger domain. Tu was identified in all 38 Wty lines examined, and was completely absent from all 56 non-warty (nWty) lines. Cucumber plants transgenic for Tu (TCP) revealed that Tu was required for the Wty fruit phenotype. Subcellular localization showed that the fusion protein GFP-Tu was localized mainly to the nucleus. Based on analyses of semi-quantitative and quantitative reverse transcription polymerase chain reaction (RT-PCR), and mRNA in situ hybridization, we found that Tu was expressed specifically in fruit spine cells during development of fruit tubercules. Moreover, cytokinin (CTK) content measurements and cytological observations in Wty and nWty fruits revealed that the Wty fruit phenotype correlated with high endogenous CTK concentrations. As a result of further analyses on the transcriptomic profile of the nWty fruit epidermis and TCP fruit warts, expression of CTK-associated genes, and hormone content in nWty fruit epidermis, Wty fruit warts and epidermis, and TCP fruit warts and epidermis, we found that Tu probably promoted CTK biosynthesis in fruit warts. Here we show that Tu could not be expressed in the glabrous and tubercule-free mutant line gl that contained Tu, this result that futher confirmed the epistatic effect of the trichome (spine) gene Gl over Tu. Taken together, these data led us to propose a genetic pathway for the Wty fruit trait that could guide future mechanistic studies.

  13. Elongation factor Tu D138N, a mutant with modified substrate specificity, as a tool to study energy consumption in protein biosynthesis.

    Science.gov (United States)

    Weijland, A; Parlato, G; Parmeggiani, A

    1994-09-06

    Substitution Asp138-->Asn changes the substrate specificity of elongation factor (EF) Tu from GTP to XTP [Hwang & Miller (1987) J. Biol. Chem. 262, 13081-13085]. This mutated EF-Tu (EF-Tu D138N) was used to show that 2 XTP molecules are hydrolyzed for each elongation cycle [Weijland & Parmeggiani (1993) Science 259, 1311-1313]. Here we extend the study of the properties of this EF-Tu mutant and its function in the elongation process. In poly(U)-directed poly(phenylalanine) synthesis, the number of peptide chains synthesized using EF-Tu D138N.XTP was 30% higher than with EF-Tu wild type (wt).GTP. However, since in the former case the average peptide chain length was correspondingly reduced, the number of the residues incorporated turned out to be nearly the same in both systems. The K'd values of the XTP and XDP complexes of EF-Tu D138N were similar to those of the GTP and GDP complexes of EF-Tu wt. The extent of leucine misincorporation and the kirromycin effect were also comparable to those in the EF-Tu wt/GTP system. The hydrolysis of two XTP molecules, very likely as part of two EF-Tu D138N.XTP complexes, for each elongation cycle was found to be independent of (i) MgCl2 concentration, (ii) ribosome concentration, and (iii) temperature (5-40 degrees C). With rate-limiting amounts of XTP the K'm of its XTPase activity corresponded to the K'm for XTP of poly(phenylalanine) synthesis (0.3-0.6 microM).(ABSTRACT TRUNCATED AT 250 WORDS)

  14. The "Da Ming Hunyi Tu": Repurposing a Ming Map in Sino-African Diplomacy

    Directory of Open Access Journals (Sweden)

    Alexander Akin

    2016-12-01

    Full Text Available In 2002, an exhibition at South Africa’s parliament included a reproduction of the Da Ming Hunyi Tu (Amalgamated map of the Great Ming, citing it as the earliest world map to depict the entire African continent. As part of its broader efforts to shape a narrative of long-standing and peaceful international relations with Africa, the People’s Republic of China formally presented a replica of this map as a gift to the South African government in conjunction with the exhibition. In official statements and popular media coverage alike, the map was described as evidence of a distinctly Chinese approach to global relations, based on benevolence and mutual respect. In particular, the map was ahistorically intertwined with the legacy of Zheng He’s diplomatic expeditions, which reached the East African coast in the early 1400s. To the cartographic historian, however, the depiction of Africa in the Da Ming Hunyi Tu is clearly derived from non-Chinese sources that predate Zheng He’s expeditions. This article examines the ways in which the map has been divorced from its original context to suit modern needs, exemplifying the deployment of cartography to deflect anxieties about the nature of Chinese economic influence in South Africa.

  15. H1 - antihistamīna preparātu aprite aptiekās

    OpenAIRE

    Palamarčuka, Nadežda

    2009-01-01

    Maģistra darba mērķis bija noskaidrot, kādi H1-antihistamīna preparāti ir vislabāk pazīstami un tiek visvairāk pirkti aptiekās. Pētījumā tika aptaujāti 100 pacienti un analizēti lieltirgotavas MagnumMedical H1-antihistamīna preparātu tirdzniecības apjomi. Iegūtie rezultāti liecina, ka vislielākais H1-antihistamīna preparātu patēriņš ir pirmās paaudzes preparātiem Tavegyl (klemastīns) un Suprastin (hloropiramīns), jo to iegādei nav nepieciešamas receptes, tie ir salīdzinoši lēti un ir bijuši p...

  16. An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells

    Energy Technology Data Exchange (ETDEWEB)

    Whitney, John C.; Quentin, Dennis; Sawai, Shin; LeRoux, Michele; Harding, Brittany N.; Ledvina, Hannah E.; Tran, Bao Q.; Robinson, Howard; Goo, Young Ah; Goodlett, David R.; Raunser, Stefan; Mougous, Joseph D.

    2015-10-08

    Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD+ and NADP+. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.

  17. TuBaFrost 5: multifunctional central database application for a European tumor bank.

    Science.gov (United States)

    Isabelle, M; Teodorovic, I; Morente, M M; Jaminé, D; Passioukov, A; Lejeune, S; Therasse, P; Dinjens, W N M; Oosterhuis, J W; Lam, K H; Oomen, M H A; Spatz, A; Ratcliffe, C; Knox, K; Mager, R; Kerr, D; Pezzella, F; van de Vijver, M; van Boven, H; Alonso, S; Kerjaschki, D; Pammer, J; Lopez-Guerrero, J A; Llombart Bosch, A; Carbone, A; Gloghini, A; van Veen, E-B; van Damme, B; Riegman, P H J

    2006-12-01

    Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect TuBaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the TuBaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre. The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails.

  18. Absolute activity determination of CaWO{sub 4} crystals

    Energy Technology Data Exchange (ETDEWEB)

    Muenster, Andrea; Ertl, Andreas; Guetlein, Achim; Lanfranchi, Jean-Come; Potzel, Walter; Roth, Sabine; Simon, Daniel; Scholl, Stephan; Sivers, Moritz von; Strauss, Raimund; Wawoczny, Stephan; Willers, Michael; Wuestrich, Marc; Zoeller, Andreas [TU Muenchen, Fakultaet fuer Physik, E15, Garching (Germany)

    2013-07-01

    The direct Dark Matter search experiment CRESST uses CaWO{sub 4} single crystals as targets for possible WIMP recoils. A particle interaction in the crystal produces phonons as well as scintillation light. As the light signal is dependent on the kind of interacting particle, a particle discrimination on an event-by-event basis is feasible. The observed background is mainly due to intrinsic radioactive impurities of the CaWO{sub 4} target. An activity of this intrinsic contamination can be determined with the investigation of α-decays in the crystal. Up to now, CaWO{sub 4} crystals were produced by suppliers in Russia and Ukraine. Since 2011 we are able to grow CaWO{sub 4} crystals in a Czochralski furnace installed in the crystal laboratory of TU Munich, which has the advantage to better meet the requirements of CRESST and to ensure the availability of CaWO{sub 4} crystals for the successive future multi-material experiment EURECA. To check the radiopurity, first self-grown crystals were investigated in test measurements. In this talk we will present the results for absolute α-activities of self-grown crystals and compare them to α-activities of the crystals installed in the last CRESST run (Run32).

  19. Loss of Thy-1 (CD90) antigen expression on mesenchymal stromal cells from hematologic malignancies is induced by in vitro angiogenic stimuli and is associated with peculiar functional and phenotypic characteristics.

    Science.gov (United States)

    Campioni, D; Lanza, F; Moretti, S; Ferrari, L; Cuneo, A

    2008-01-01

    Little is known about human mesenchymal stromal cell (hMSC) phenotypic and functional subsets in response to environmental stimuli. The strategy used in this study focused on defining hMSC functional subpopulations based in particular on their Thy-1 (CD90) antigen (Ag) surface expression. The effect of different in vitro microenvironmental conditions on the isolation and expansion of bone marrow-derived (BM) hMSC from hematologic malignancies (HM) and normal samples (NS) was assayed. hMSC clonogenic and differentiation potential, phenotypic profile and long-term capacity to sustain in vitro hemopoiesis were considered in relation to the different expansion protocols. The results showed that angiogenic supplements used in combination with low serum content gave rise to the appearance of Thy-1(-) HM-MSC with high proliferative potential, capable of restoring the typical HM stromal impairment. The expression of the CD271 was partially maintained. We further report an enhancement towards the osteogenic and adipogenic differentiation capacity by the Thy-1(-) HM-MSC subset. Despite the angiogenic treatment, the Thy-1(-) MSC stopped short of full endothelial differentiation. In this paper we provide evidence that in vitro angiogenic stimuli generate HM-MSC lacking CD90 Ag expression. The Thy-1(-) MSC subset is characterized by peculiar functional and phenotypic characteristics, thus supporting the role played by the microenvironment in selecting particular hMSC subsets maintaining normal tissue homeostasis or inducing pathologic processes.

  20. Structure of ThiM from Vitamin B1 biosynthetic pathway of Staphylococcus aureus - Insights into a novel pro-drug approach addressing MRSA infections

    Science.gov (United States)

    Drebes, Julia; Künz, Madeleine; Windshügel, Björn; Kikhney, Alexey G.; Müller, Ingrid B.; Eberle, Raphael J.; Oberthür, Dominik; Cang, Huaixing; Svergun, Dmitri I.; Perbandt, Markus; Betzel, Christian; Wrenger, Carsten

    2016-03-01

    Infections caused by the methicillin-resistant Staphylococcus aureus (MRSA) are today known to be a substantial threat for global health. Emerging multi-drug resistant bacteria have created a substantial need to identify and discover new drug targets and to develop novel strategies to treat bacterial infections. A promising and so far untapped antibiotic target is the biosynthesis of vitamin B1 (thiamin). Thiamin in its activated form, thiamin pyrophosphate, is an essential co-factor for all organisms. Therefore, thiamin analogous compounds, when introduced into the vitamin B1 biosynthetic pathway and further converted into non-functional co-factors by the bacterium can function as pro-drugs which thus block various co-factor dependent pathways. We characterized one of the key enzymes within the S. aureus vitamin B1 biosynthetic pathway, 5-(hydroxyethyl)-4-methylthiazole kinase (SaThiM; EC 2.7.1.50), a potential target for pro-drug compounds and analyzed the native structure of SaThiM and complexes with the natural substrate 5-(hydroxyethyl)-4-methylthiazole (THZ) and two selected substrate analogues.

  1. Transgene therapy for rat anti-Thy1.1 glomerulonephritis via mesangial cell vector with a polyethylenimine/decorin nanocomplex

    Science.gov (United States)

    Sun, Jian-Yong; Sun, Yu; Wu, Hui-Juan; Zhang, Hong-Xia; Zhao, Zhong-Hua; Chen, Qi; Zhang, Zhi-Gang

    2012-08-01

    Polyethylenimine (PEI), a cationic polymer, is one of the most efficient non-viral vectors for transgene therapy. Decorin (DCN), a leucine-rich proteoglycan secreted by glomerular mesangial cells (MC), is a promising anti-fibrotic agent for the treatment of glomerulonephritis. In this study, we used PEI-DCN nanocomplexes with different N/P ratios to transfect MC in vitro and deliver the MC vector with PEI-DCN expressing into rat anti-Thy1.1 nephritis kidney tissue via injection into the left renal artery in vivo. The PEI-plasmid DNA complex at N/P 20 had the highest level of transfection efficiency and the lowest level of cytotoxicity in cultured MC. Following injection, the ex vivo gene was transferred successfully into the glomeruli of the rat anti-Thy1.1 nephritis model by the MC vector with the PEI-DCN complex. The exogenous MC with DCN expression was located mainly in the mesangium and the glomerular capillary. Over-expression of DCN in diseased glomeruli could result in the inhibition of collagen IV deposition and MC proliferation. The pathological changes of rat nephritis were alleviated following injection of the vector. These findings demonstrate that the DCN gene delivered by the PEI-DNA nanocomplex with the MC vector is a promising therapeutic method for the treatment of glomerulonephritis.

  2. Review: Hu Houxuan, "Xia and the Tu State in the Oracle Inscriptions: A Consideration" Gudai Chengbang Shi Yanjiu (Studies on Ancient City States) Renmin Press, 1989, Pp. 340-353.

    Institute of Scientific and Technical Information of China (English)

    RiZhi

    1995-01-01

    This article verified that the Tu state mentioned in the oracle inscriptions was the Xia people after the Xia Dynasty had been conquered by Tang, the first king of the Shang Dynasty m c.a. 16th century B.C. The state of Xia, which originated from Yu, was entitled "Xia Hou"; its king was hence called "Xia Hou Di" (Emperor of Xia Hou), e.g."Xia Hou Di Qi". The Xia people, together with the Yin (Shang) people, and the Zhou people, were the so-called "people of San Dai" (Three Dynasties). As mentioned in "Lunyu, Bayi", they founded the states, with the Xia people being associated with the pine, the Yin people with the cypress, and the Zhou people with the chestnut.

  3. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation.

    Science.gov (United States)

    Choudhury, Nila Roy; Nowak, Jakub S; Zuo, Juan; Rappsilber, Juri; Spoel, Steven H; Michlewski, Gracjan

    2014-11-20

    RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation. Surprisingly, despite binding to other pre-microRNAs (pre-miRNAs), only pre-let-7 is efficiently uridylated by TuT4. Thus, we hypothesized the existence of substrate-specific cofactors that stimulate Lin28a-mediated pre-let-7 uridylation or restrict its functionality on non-let-7 pre-miRNAs. Through RNA pull-downs coupled with quantitative mass spectrometry, we identified the E3 ligase Trim25 as an RNA-specific cofactor for Lin28a/TuT4-mediated uridylation. We show that Trim25 binds to the conserved terminal loop (CTL) of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. These findings reveal that protein-modifying enzymes, only recently shown to bind RNA, can guide the function of canonical ribonucleoprotein (RNP) complexes in cis, thereby providing an additional level of specificity.

  4. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation

    Directory of Open Access Journals (Sweden)

    Nila Roy Choudhury

    2014-11-01

    Full Text Available RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation. Surprisingly, despite binding to other pre-microRNAs (pre-miRNAs, only pre-let-7 is efficiently uridylated by TuT4. Thus, we hypothesized the existence of substrate-specific cofactors that stimulate Lin28a-mediated pre-let-7 uridylation or restrict its functionality on non-let-7 pre-miRNAs. Through RNA pull-downs coupled with quantitative mass spectrometry, we identified the E3 ligase Trim25 as an RNA-specific cofactor for Lin28a/TuT4-mediated uridylation. We show that Trim25 binds to the conserved terminal loop (CTL of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. These findings reveal that protein-modifying enzymes, only recently shown to bind RNA, can guide the function of canonical ribonucleoprotein (RNP complexes in cis, thereby providing an additional level of specificity.

  5. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    NARCIS (Netherlands)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

    2008-01-01

    Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

  6. 2011 Lasker Clinical Medical Research Award Honors Prof.TU You-you for the Discovery of Artemisinin

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The 2011 Lasker Clinical Medical Research Award honors a scientist who discovered artemisinin and its utility for treating malaria.Prof.TU You-you (China Academy of Chinese Medical Sciences,Beijing) transformed an ancient Chinese healing method into the most powerful antimalarial medicine currently available that has saved millions of lives across the globe,especially in the developing world.

  7. A guidelines handbook for GPR surveys in tunnels: a COST Action TU1208 contribution

    Science.gov (United States)

    Bianchini Ciampoli, Luca; Alani, Amir M.; Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    A significant open issue concerning the reliability of geophysical methods and in particular of ground penetrating radar (GPR), both in research and professional context, is a general lack of international standards. This is a major problem to be faced, in order to gain scientific strictness for the GPR practices, and to easily extend to the international community the results achieved within the area of single virtuous countries. Producing international guidelines can represent an important step forward, in this sense. In the memorandum of understanding of the COST Action TU1208 is clearly stated that one of the main purposes of the Action is the "development of innovative protocols and guidelines which will be published in a handbook and constitute a basis for European Standards, for an effective GPR application in CE tasks; safety, economic and financial criteria will be integrated within the protocols". Of course this is not a simple task to be accomplished. Firstly, survey procedures are highly dependent on the objective of the survey itself. On the basis of the objective of each geophysical test, the GPR system, the antenna configuration, and even the processing procedures may change. Besides, these procedures are also influenced by the environmental conditions in which the tests are performed. This affects several aspects spanning from hardware to software, but including, for instance, also safety issues. Due to these reasons, one of the main goal of the COST Action TU1208 is the development of several guidelines related to the main applications of GPR in the field of civil engineering. In this work, the structure of a guidelines handbook for GPR activities in tunnels is outlined. In the first sections, the principal references in the field are provided, and the most common GPR equipment and complementary technologies are described. Subsequently, the survey methodologies are explained. Particular attention is paid to the preliminary activities to be carried

  8. Preventive Effect of TU-100 on a Type-2 Model of Colitis in Mice: Possible Involvement of Enhancing Adrenomedullin in Intestinal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Atsushi Kaneko

    2013-01-01

    Full Text Available Purpose. Crohn's disease (CD and ulcerative colitis (UC, the two major forms of inflammatory bowel disease (IBD, have histopathologically and immunologically different characteristics. We previously reported that a traditional Japanese medicine, daikenchuto (TU-100, ameliorated a trinitrobenzenesulfonic acid- (TNBS- induced type-1 model colitis exhibiting histopathological features of CD through adrenomedullin (ADM enhancement. Our current aims were to examine whether TU-100 ameliorates a type-2 model colitis that histologically resembles UC and identify the active ingredients. Methods. TU-100 was administered orally to mice with oxazolone- (OXN- induced type-2 model colitis. The morbidity was evaluated by body weight loss and the macroscopic score of colonic lesions. ADM was quantified using an EIA kit. Results. TU-100 prevented weight loss and colon ulceration. ADM production by intestinal epithelial cells was increased by TU-100 addition. Screening to identify active ingredients showed that [6]-shogaol and hydroxy α-sanshool enhanced ADM production. Conclusions. TU-100 exerted a protective effect in OXN-induced type-2 model colitis, indicating that TU-100 may be a beneficial agent for treatment of UC.

  9. Primjena industrijskog otpada CaF2 u procesu ozelenjivanja odlagališta fosfogipsa

    OpenAIRE

    Leaković, S.; Lisac, H.; Vukadin, R.

    2012-01-01

    U procesu proizvodnje fosforne kiseline nastaje fosfogips kao sporedni produkt reakcije sirovog fosfata i sumporne kiseline. On se kao neopasni otpad trajno odlaže na posebno uređenom odlagalištu. Prilikom proizvodnje fosforne kiseline nastaje i otpadna voda onečišćena fluoridima i fosforom. Takva otpadna voda se prije ispuštanja u prirodni prijamnik obrađuje neutralizacijom vapnenim hidratom. Nastali produkt neutralizacije je kalcijev fluorid (CaF2), koji se kao neopasni otpad odlaže u po...

  10. High Level of Agreement between Electronic and Paper Mode of Administration of a Thyroid-Specific Patient-Reported Outcome, ThyPRO

    DEFF Research Database (Denmark)

    Rasmussen, Sofie Larsen; Rejnmark, Lars; Ebbehøj, Eva

    2016-01-01

    INTRODUCTION AND PURPOSE: Use of electronic questionnaires to collect health-related quality-of-life data has evolved as an alternative to paper questionnaires. For the electronic questionnaire to be used interchangeably with the validated paper questionnaire, measurement properties similar...... to the original must be demonstrated. The aim of the present study was to assess the equivalence between the paper version and the electronic version of the thyroid-related quality-of-life questionnaire ThyPRO. METHODS: Patients with Graves' hyperthyroidism or autoimmune hypothyroidism in a clinically stable...... with data from a previous paper-paper test-retest study. RESULTS: 104 patients were included. ICCs were generally high for the 13 scales, ranging from 0.76 to 0.95. There was a small but significant difference in the scale score between paper and electronic administration for the Cosmetic complaints scale...

  11. Cross-cultural validity of the thyroid-specific quality-of-life patient-reported outcome measure, ThyPRO

    DEFF Research Database (Denmark)

    Watt, Torquil; Barbesino, Giuseppe; Bjørner, Jakob

    2015-01-01

    BACKGROUND AND PURPOSE: Thyroid diseases are common and often affect quality of life (QoL). No cross-culturally validated patient-reported outcome measuring thyroid-related QoL is available. The purpose of the present study was to test the cross-cultural validity of the newly developed thyroid...... = 148), Denmark (n = 902) and Sweden (n = 187). Translated versions were compared pairwise to the English version by examining uniform and nonuniform DIF, i.e., whether patients from different countries respond differently to a particular item, although they have identical level of the concept measured...... scale scores, most of which could be explained by sample differences not controlled for. CONCLUSION: The ThyPRO has good cross-cultural validity with only minor cross-cultural invariance and is recommended for use in international multicenter studies....

  12. High Level of Agreement between Electronic and Paper Mode of Administration of a Thyroid-Specific Patient-Reported Outcome, ThyPRO

    Science.gov (United States)

    Rasmussen, Sofie Larsen; Rejnmark, Lars; Ebbehøj, Eva; Feldt-Rasmussen, Ulla; Rasmussen, Åse Krogh; Bjorner, Jakob Bue; Watt, Torquil

    2016-01-01

    Introduction and Purpose Use of electronic questionnaires to collect health-related quality-of-life data has evolved as an alternative to paper questionnaires. For the electronic questionnaire to be used interchangeably with the validated paper questionnaire, measurement properties similar to the original must be demonstrated. The aim of the present study was to assess the equivalence between the paper version and the electronic version of the thyroid-related quality-of-life questionnaire ThyPRO. Methods Patients with Graves' hyperthyroidism or autoimmune hypothyroidism in a clinically stable phase were included. The patients were recruited from two endocrine outpatient centers. All patients completed both versions in a randomized test-retest set-up. Scores were compared using intraclass correlation coefficients (ICCs), paired t tests and Bland-Altman plots. Limits of agreement were compared with data from a previous paper-paper test-retest study. Results 104 patients were included. ICCs were generally high for the 13 scales, ranging from 0.76 to 0.95. There was a small but significant difference in the scale score between paper and electronic administration for the Cosmetic complaints scale, but no differences were found for any other scale. Bland-Altman plots showed similar limits of agreement compared to the earlier test-retest study of the paper version of ThyPRO. Conclusion Based on our analyses using ICCs, paired t tests and Bland-Altman plots, we found adequate agreement between the paper and electronic questionnaires. The statistically significant difference in score found in the Cosmetic complaints scale is small and probably clinically insignificant. PMID:27099841

  13. Thr202Ala in thyA Is a Marker for the Latin American Mediterranean Lineage of the Mycobacterium tuberculosis Complex Rather than Para-Aminosalicylic Acid Resistance

    KAUST Repository

    Feuerriegel, S.

    2010-08-30

    Single nucleotide polymorphisms (SNPs) involved in the development of resistance represent powerful markers for the rapid detection of first- and second-line resistance in clinical Mycobacterium tuberculosis complex (MTBC) isolates. However, the association between particular mutations and phenotypic resistance is not always clear-cut, and phylogenetic SNPs have been misclassified as resistance markers in the past. In the present study, we investigated the utility of a specific polymorphism in thyA (Thr202Ala) as a marker for resistance to para-aminosalicyclic acid (PAS). Sixty-three PAS-susceptible MTBC strains comprising all major phylogenetic lineages, reference strain H37Rv, and 135 multidrug-resistant (MDR) strains from Germany (comprising 8 PAS-resistant isolates) were investigated for the presence of Thr202Ala. In both strain collections, the Thr202Ala SNP was found exclusively in strains of the Latin American Mediterranean (LAM) lineage irrespective of PAS resistance. Furthermore, PAS MICs (0.5 mg/liter) for selected LAM strains (all containing the SNP) and non-LAM strains (not containing the SNP), as well as the results of growth curve analyses performed in liquid 7H9 medium in the presence of increasing PAS concentrations (0 to 2.0 mg/liter), were identical. In conclusion, our data demonstrate that the Thr202Ala polymorphism in thyA is not a valid marker for PAS resistance but, instead, represents a phylogenetic marker for the LAM lineage of the M. tuberculosis complex. These findings challenge some of the previous understanding of PAS resistance and, as a consequence, warrant further in-depth investigations of the genetic variation in PAS-resistant clinical isolates and spontaneous mutants.

  14. Ecology of phlebotomine sand flies in the rural community of Mont Rolland (Thies region, Senegal: area of transmission of canine leishmaniasis.

    Directory of Open Access Journals (Sweden)

    Massila W Senghor

    Full Text Available BACKGROUND: Different epidemiological studies previously indicated that canine leishmaniasis is present in the region of Thiès (Senegal. However, the risks to human health, the transmission cycle and particularly the implicated vectors are unknown. METHODOLOGY/PRINCIPAL FINDINGS: To improve our knowledge on the population of phlebotomine sand flies and the potential vectors of canine leishmaniasis, sand flies were collected using sticky traps, light traps and indoor spraying method using pyrethroid insecticides in 16 villages of the rural community of Mont Rolland (Thiès region between March and July 2005. The 3788 phlebotomine sand flies we collected (2044 males, 1744 females were distributed among 9 species of which 2 belonged to the genus Phlebotomus: P. duboscqi (vector of cutaneous leishmaniasis in Senegal and P. rodhaini. The other species belonged to the genus Sergentomyia: S. adleri, S. clydei, S. antennata, S. buxtoni, S. dubia, S. schwetzi and S. magna. The number of individuals and the species composition differed according to the type of trap, suggesting variable, species-related degrees of endophily or exophily. The two species of the genus Phlebotomus were markedly under-represented in comparison to the species of the genus Sergentomyia. This study also shows a heterogeneous spatial distribution within the rural community that could be explained by the different ecosystems and particularly the soil characteristics of this community. Finally, the presence of the S. dubia species appeared to be significantly associated with canine leishmaniasis seroprevalence in dogs. CONCLUSIONS/SIGNIFICANCE: Our data allow us to hypothesize that the species of the genus Sergentomyia and particularly the species S. dubia and S. schwetzi might be capable of transmitting canine leishmaniasis. These results challenge the dogma that leishmaniasis is exclusively transmitted by species of the genus Phlebotomus in the Old World. This hypothesis should be

  15. Pentoxifylline Attenuates Proteinuria in Anti-Thy1 Glomerulonephritis via Downregulation of Nuclear Factor-κB and Smad2/3 Signaling

    Science.gov (United States)

    Chen, Yung-Ming; Chiang, Wen-Chih; Yang, Yalin; Lai, Chun-Fu; Wu, Kwan-Dun; Lin, Shuei-Liong

    2015-01-01

    Anti-Thy1 glomerulonephritis is a rat nephritis model closely simulating human mesangial proliferative glomerulonephritis. It affects primarily the mesangium, yet displays substantial proteinuria during the course. This study investigated the molecular signals underlying proteinuria in this disease and the modulation of which by the known antiproteinuric agent, pentoxifylline. Male Wistar rats were randomly divided into a control group and nephritic groups with or without treatment with IMD-0354 (an IκB kinase inhibitor), SB431542 (an activin receptor–like kinase inhibitor) or pentoxifylline. Kidney sections were prepared for histological examinations. Glomeruli were isolated for mRNA and protein analysis. Urine samples were collected for protein and nephrin quantitation. One day after nephritis induction, proteinuria developed together with ultrastructural changes of the podocyte and downregulation of podocyte mRNA and protein expression. These were associated with upregulation of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β/activins mRNAs and activation of nuclear factor (NF)-κB p65 and Smad2/3. IMD-0354 attenuated proteinuria on d 1, whereas SB431542 decreased proteinuria on d 3 and 5, in association with partial restoration of downregulated podocyte mRNA and protein expression. Pentoxifylline attenuated proteinuria and nephrinuria through the course, plus inhibition of p-NF-κB p65 (d 1) and p-Smad2/3 (d 5) and partial reversal of downregulated podocyte mRNA and protein. Our data show that the pathogenesis of proteinuria in anti-Thy1 glomerulonephritis involves TNF-α and TGF-β/activin pathways, and the evolution of this process can be attenuated by pentoxifylline via downregulation of NF-κB and Smad signals and restoration of the podocyte component of the glomerular filtration barrier. PMID:25879629

  16. Mutation of the conserved Gly94 and Gly126 in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Kjaersgård, I V; Wiborg, O

    1995-01-01

    All guanine-nucleotide-binding proteins cycle between an inactive, GDP-bound and an active, GTP-bound conformation whereby they function as molecular switches. Elongation factor Tu from Escherichia coli is used as a model for defining residues important in the switch mechanism. Gly94 and Gly126...... for GDP over GTP. Furthermore the [G94A]EF-Tu mutant possesses an increased GTPase activity. The aminoacyl-tRNA affinity is much reduced for [G94A]EF-Tu, as reflected in an increase of the dissociation rate constant for the ternary complex by a factor of 40. Surprisingly, however, both mutants...... in their GDP forms have a low, but significant affinity for aminoacyl-tRNA, which is not seen for the wild-type elongation factor Tu. The mutants only exhibit minor changes compared to the wild type with respect to in vitro translation of a poly(U) messenger. Udgivelsesdato: 1995-Feb-15...

  17. Collaboration of the Dutch research program for radioactive waste disposal (OPERA) and TU Delft

    Energy Technology Data Exchange (ETDEWEB)

    Bykov, D.M.; Kloosterman, J.L. [TU Delft (Netherlands). Reactor Inst. Delft; Neeft, E.A.C.; Verhoef, E.V. [COVRA N.V., Nieuwdorp (Netherlands)

    2015-07-01

    awarded research proposals available at www.covra.nl/downloads/o pera. Building up competences and knowledge on radioactive waste management and geological disposal is an important goal of OPERA. To this end OPERA collaborates with TU Delft to develop an academic curriculum for the chair Chemistry of the Nuclear Fuel Cycle in the Master of Science Engineering. Furthermore, the results obtained in OPERA will be presented at a Summer School which is planned to be organized at the end of the Program. TU Delft educates young specialists, bachelor and master students, by research projects on geological disposal at the new actinide laboratory (U and Th). For such purpose the knowledge transfer from other OPERA partners is also foreseen, including students internships and visits of the research facilities. Reactor Institute Delft (RID) is part of the Applied Sciences faculty of TU Delft and houses the Radiation Science and Technology (RST) department. RID operates the Hoger Onderwijs Reactor (HOR), a 2 MW pool- type research reactor in an academic setting. The reactor is used as a source of neutrons and positrons for research purposes, including those of OPERA. It also provides neutrons to a variety of facilities for radioisotope production and neutron activation analysis. OPERA is financed by the Dutch Ministry of Economic Affairs and the public limited liability company Elektriciteits-Produktiemaatschappij Zuid-Nederland (EPZ) and coordinated by COVRA.

  18. Development of SAP-DoA techniques for GPR data processing within COST Action TU1208

    Science.gov (United States)

    Meschino, Simone; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work focuses on the use of Sub-Array Processing (SAP) and Direction of Arrival (DoA) approaches for the processing of Ground-Penetrating Radar data, with the purpose of locating metal scatterers embedded in concrete or buried in the ground. Research activities have been carried out during two Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in May 2015 and January 2016. In applications involving smart antennas and in the presence of several transmitters operating simultaneously, it is important for a receiving array to be able to estimate the Direction of Arrival (DoA) of the incoming signals, in order to decipher how many emitters are present and predict their positions. A number of methods have been devised for DoA estimation: the MUltiple SIgnal Classification (MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique (ESPRIT) are amongst the most popular ones [1]. In the scenario considered by us, the electromagnetic sources are the currents induced on metal elements embedded in concrete or buried in the ground. GPR radargrams are processed, to estimate the DoAs of the electric field back-scattered by the sought targets. In order to work in near-field conditions, a sub-array processing (SAP) approach is adopted: the radargram is partitioned in sub-radargrams composed of few A-scans each, the dominant DoA is predicted for each sub-radargram. The estimated angles are triangulated, obtaining a set of crossings with intersections condensed around object locations. This pattern is filtered, in order to remove a noisy background of unwanted crossings, and is processed by applying the statistical procedure described in [2]. We tested our approach on synthetic GPR radargrams, obtained by using the freeware simulator gprMax implementing the Finite-Difference Time-Domain method [3]. In particular, we worked with

  19. La ciudad-mito y lo grotesco en los hermanos González Tuñón

    OpenAIRE

    2015-01-01

    La poética de los hermanos González Tuñón, iniciada al calor de las vanguardias porteñas, es estudiada atendiendo a la mítica de lo urbano, lo que permite a su vez analizar el empleo de la estética grotesca como modo de subrayar una cultura híbrid

  20. BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape

    Directory of Open Access Journals (Sweden)

    Quan Chen

    2015-07-01

    Full Text Available Virus diseases greatly affect oilseed rape (Brassica napus production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov and BnSGS3-silenced (BnSGS3-Si oilseed rape (cv. Zhongshuang No. 6 plants. These three viruses are Oilseed rape mosaic virus (ORMV, Turnip mosaic virus (TuMV and Cucumber mosaic virus (CMV. The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation for breeding virus-resistant oilseed rape varieties.

  1. EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Method: Human pancreatic cancer cell line PaTu8988s in exponential growth stage was used to study the effect of different drug concentrations on the cell line in the presence of different concentrations (0 μg/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml, 10 μg/m, 200 μg/ml) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out. Results: The inhibitory rate on the cell line PaTu8988s was 98.73%,95.76%,69.49%,33.05% and 0 for ASODM concentrations of 200 μg/ml,100 μg/ml,50 μg/ml,25 μg/ml and 5 μg/ml at 48 hours. Conclusions: K-ras complementary ASODN can inhibit the growth of human pancreatic cancer cell line PaTu8988s by 30.05% to 98.73%. This is likely to contribute to the specificity of the K-ras mRNA complementary capped ASODN sequential codon. Non-specific effect and side effect of ASOND were observed for instance, the greater the concentration is, the earlier the peak of inhibitory rate is reached. In concentration of 25 μg/ml to 100 μg/ml ASODN showed a dose-effect correlation

  2. A conserved P-loop anchor limits the structural dynamics that mediate nucleotide dissociation in EF-Tu

    Science.gov (United States)

    Mercier, Evan; Girodat, Dylan; Wieden, Hans-Joachim

    2015-01-01

    The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding properties. Understanding the underlying design principles is of great interest for biomolecular engineering applications. We have used rapid-kinetics measurements in vitro and molecular dynamics (MD) simulations in silico to investigate the relationship between GTP-binding properties and P-loop structural dynamics in the universally conserved Elongation Factor (EF) Tu. Analysis of wild type EF-Tu and variants with substitutions at positions in or adjacent to the P-loop revealed a correlation between P-loop flexibility and the entropy of activation for GTP dissociation. The same variants demonstrate more backbone flexibility in two N-terminal amino acids of the P-loop during force-induced EF-Tu·GTP dissociation in Steered Molecular Dynamics simulations. Amino acids Gly18 and His19 are involved in stabilizing the P-loop backbone via interactions with the adjacent helix C. We propose that these P-loop/helix C interactions function as a conserved P-loop anchoring module and identify the presence of P-loop anchors within several GTPases and ATPases suggesting their evolutionary conservation. PMID:25566871

  3. A conserved P-loop anchor limits the structural dynamics that mediate nucleotide dissociation in EF-Tu

    Science.gov (United States)

    Mercier, Evan; Girodat, Dylan; Wieden, Hans-Joachim

    2015-01-01

    The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding properties. Understanding the underlying design principles is of great interest for biomolecular engineering applications. We have used rapid-kinetics measurements in vitro and molecular dynamics (MD) simulations in silico to investigate the relationship between GTP-binding properties and P-loop structural dynamics in the universally conserved Elongation Factor (EF) Tu. Analysis of wild type EF-Tu and variants with substitutions at positions in or adjacent to the P-loop revealed a correlation between P-loop flexibility and the entropy of activation for GTP dissociation. The same variants demonstrate more backbone flexibility in two N-terminal amino acids of the P-loop during force-induced EF-Tu.GTP dissociation in Steered Molecular Dynamics simulations. Amino acids Gly18 and His19 are involved in stabilizing the P-loop backbone via interactions with the adjacent helix C. We propose that these P-loop/helix C interactions function as a conserved P-loop anchoring module and identify the presence of P-loop anchors within several GTPases and ATPases suggesting their evolutionary conservation.

  4. Electromagnetic simulators for Ground Penetrating Radar applications developed in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Warren, Craig; Antonijevic, Sinisa; Doric, Vicko; Poljak, Dragan

    2017-04-01

    Founded in 1971, COST (European COoperation in Science and Technology) is the first and widest European framework for the transnational coordination of research activities. It operates through Actions, science and technology networks with a duration of four years. The main objective of the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (4 April 2013 - 3 October 2017) is to exchange and increase knowledge and experience on Ground-Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe a wider use of this technique. Research activities carried out in TU1208 include all aspects of the GPR technology and methodology: design, realization and testing of radar systems and antennas; development and testing of surveying procedures for the monitoring and inspection of structures; integration of GPR with other non-destructive testing approaches; advancement of electromagnetic-modelling, inversion and data-processing techniques for radargram analysis and interpretation. GPR radargrams often have no resemblance to the subsurface or structures over which the profiles were recorded. Various factors, including the innate design of the survey equipment and the complexity of electromagnetic propagation in composite scenarios, can disguise complex structures recorded on reflection profiles. Electromagnetic simulators can help to understand how target structures get translated into radargrams. They can show the limitations of GPR technique, highlight its capabilities, and support the user in understanding where and in what environment GPR can be effectively used. Furthermore, electromagnetic modelling can aid the choice of the most proper GPR equipment for a survey, facilitate the interpretation of complex datasets and be used for the design of new antennas. Electromagnetic simulators can be employed to produce synthetic radargrams with the purposes of testing new data-processing, imaging and inversion algorithms, or assess

  5. [Control of Aedes aegypti breeding sites with the program Recicla por tu bienestar in Merida, Mexico].

    Science.gov (United States)

    Barrera-Pérez, Mario A; Pavía-Ruz, Norma; Mendoza-Mezquita, Jorge Eduardo; Torres-Arcila, Nerio; Hernández-Hernández, Ramón; Castro-Gamboa, Francisco; Geded-Moreno, Eduardo; Cohuo-Rodríguez, Azael; Medina-Barreiro, Anuar; Koyoc-Cardeña, Edgar; Gómez-Dantés, Héctor; Kroeger, Axel; Vázquez-Prokopec, Gonzalo; Manrique-Saide, Pablo

    2015-01-01

    To determine the importance of Ae. aegypti breeding-sites in Merida;to evaluate the impact of Recicla por tu bienestar (RxB, a recycling program) on the reduction of breeding sites and the perception of participants. The relative importance for pupae production of the different types of breeding-sites was determined. Pre-and post-RxB entomological surveys were performed in participant neighborhoods to evaluate the impact on total containers and positive breeding-sites. A survey on the perception of participating people about dengue prevention and control and RxB was applied. Buckets/pots and "small diverse items" were the most important breeding-sites. RxB had a significant impact in the reduction of total containers (IRR = 0.74), positive containers (IRR = 0.33) and the risk of a house being positive for Ae. aegypti (OR = 0.41). All the interviewed participants referred RxB as needed and most consider it useful. RxB should be considered as a good practice for the dengue vector control.

  6. TU Comae Berenices : Blazhko RR Lyrae Star in a Potential Binary System

    CERN Document Server

    de Ponthiere, Pierre; Menzies, Kenneth; Sabo, Richard

    2016-01-01

    We present the results of a photometry campaign of TU Com performed over a five-year time span. The analysis showed that the possible Blazhko period of 75 days published by the General Catalogue of Variable Stars is not correct. We identified two Blazhko periods of 43.6 and 45.5 days. This finding is based on measurement of 124 light maxima. A spectral analysis of the complete light curve confirmed these two periods. Besides the Blazhko amplitude and phase modulations, another long term periodic phase variation has been identified. This long term periodic variation affects the times of maximum light only and can be attributed to a light-travel time effect due to orbital motion of a binary system. The orbital parameters have been estimated by a nonlinear least-square fit applied to the set of (O-C) values. The Levenberg-Marquart algorithm has been used to perform the nonlinear least-square fit. The tentative orbital parameters include an orbital period of 1676 days, a minimal semi-major axis of 1.55 AU and a s...

  7. Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.

    Science.gov (United States)

    Greimers, R; Trebak, M; Moutschen, M; Jacobs, N; Boniver, J

    1996-03-01

    A visible-light, dual-laser, flow cytometric method was developed for the simultaneous analysis of intracellular ionized calcium concentration ([Ca2+]i) and three cell-surface markers (CD4, CD8, and Thy-1.2 antigens) by using the calcium probe fluo-3 and using R-phycoerythrin (PE), peridinin chlorophyll-alpha protein (PerCP), and allophycocyanin (APC) conjugated monoclonal antibodies (MoAbs). This improved method was used in the analysis of [Ca2+]i mobilization upon in vitro stimulation with mitogenic lectins [phytohaemagglutinin (PHA) or concanavalin A (ConA)], anti-CD3 MoAbs, or A23187 calcium ionophore in the heterogeneous lymph node cell populations from healthy C57BL/Ka mice. The present results show that the calcium responses were heterogeneous and dependent on the cellular immunophenotype, not only on lectins or anti-CD3 MoAbs stimulation, but also on the receptor-independent A23187 ionophore stimulation. An in situ fluo-3 calibration method (using A23187 and metabolic poisons in Ca2+ /EGTA buffers with known free calcium concentrations) indicated a resting [Ca2+]i in lymphocytes of 103 +/- 23 nM (mean +/- S.D.) but with significant differences between the [Ca2+]i in B cells and in all of the T-cell subsets (CD4+Thy-1+, CD4+Thy-1-, and CD8+T cells). Both the B cells and the T-cell subsets showed an increase of fluo-3 fluorescence upon in vitro stimulation with ConA or PHA, but the calcium mobilization following lectin stimulation was time delayed in all T-cell subsets. Only the T cells, including the CD4+Thy-1- subset, responded to anti-CD3 MoAbs. The percentage of responding cells upon stimulation with ConA was higher in T cells than in B cells. By contrast, PHA gave a higher response in B cells. After stimulation with different mitogens, [Ca2+]i increased in both CD4+ and CD8+ T-cell subsets. However, the percentage of responding cells was far higher in the CD4+Thy-1+ subset than in the CD4+Thy-1- or the CD8+T-cell subsets. The stimulation with A23187

  8. TU-100 (Daikenchuto) and ginger ameliorate anti-CD3 antibody induced T cell-mediated murine enteritis: microbe-independent effects involving Akt and NF-κB suppression.

    Science.gov (United States)

    Ueno, Nobuhiro; Hasebe, Takumu; Kaneko, Atsushi; Yamamoto, Masahiro; Fujiya, Mikihiro; Kohgo, Yutaka; Kono, Toru; Wang, Chong-Zhi; Yuan, Chun-Su; Bissonnette, Marc; Chang, Eugene B; Musch, Mark W

    2014-01-01

    The Japanese traditional medicine daikenchuto (TU-100) has anti-inflammatory activities, but the mechanisms remain incompletely understood. TU-100 includes ginger, ginseng, and Japanese pepper, each component possessing bioactive properties. The effects of TU-100 and individual components were investigated in a model of intestinal T lymphocyte activation using anti-CD3 antibody. To determine contribution of intestinal bacteria, specific pathogen free (SPF) and germ free (GF) mice were used. TU-100 or its components were delivered by diet or by gavage. Anti-CD3 antibody increased jejunal accumulation of fluid, increased TNFα, and induced intestinal epithelial apoptosis in both SPF and GF mice, which was blocked by either TU-100 or ginger, but not by ginseng or Japanese pepper. TU-100 and ginger also blocked anti-CD3-stimulated Akt and NF-κB activation. A co-culture system of colonic Caco2BBE and Jurkat-1 cells was used to examine T-lymphocyte/epithelial cells interactions. Jurkat-1 cells were stimulated with anti-CD3 to produce TNFα that activates epithelial cell NF-κB. TU-100 and ginger blocked anti-CD3 antibody activation of Akt in Jurkat cells, decreasing their TNFα production. Additionally, TU-100 and ginger alone blocked direct TNFα stimulation of Caco2BBE cells and decreased activation of caspase-3 and polyADP ribose. The present studies demonstrate a new anti-inflammatory action of TU-100 that is microbe-independent and due to its ginger component.

  9. Fusion calculations for 40Ca+40Ca, 48Ca+48Ca, 40Ca+48Ca and p+208Pb systems

    Science.gov (United States)

    Gao, Jie; Zhang, Haifei; Bao, Xiaojun; Li, Junqing; Zhang, Hongfei

    2014-09-01

    The fusion cross sections of calcium isotopes and proton induced fusion have been calculated in terms of a coupled-channels formulation. Results indicated that there are big differences between the two fusion types. In the calculations of calcium isotopes fusion, the pair-transfer coupling has been applied in addition to the vibrational coupling, the combined effects showed that pair-transfer has played a significant role in the fusion process for the asymmetric 40Ca+48Ca system. The result of proton induced fusion for p+208Pb system successfully presents the fusion oscillation, which agrees with the experimental data rather well.

  10. [Leprosy in children in the region of Thiès, Senegal: study determining whether or not it is a signal of recrudescence].

    Science.gov (United States)

    Dioussé, Pauline; Dione, Haby; Bammo, Mariama; Gueye, Ndiaga; Diallo, Thierno Abdoul Aziz; Seck, Fatou; Gueye, Ramatoulaye Diagne; Dieng, Mame Thierno; Fall, Fatma Sarr; Diop, Moustapha; Diop, Bernard Marcel; Ka, Mamadou Mourtalla

    2017-01-01

    Leprosy is an infectious and transmissible disease. According to the WHO, the number of new cases of leprosy in children in Senegal has risen moderately since 2013. This study aimed to analyze the epidemiological, clinical, therapeutic and evolutionary features of leprosy in children in the geographical areas of two social rehabilitation villages in the region of Thiès. We conducted a retrospective study over a period of 3 years (2013-2015). All new cases of Hansen's disease aged 0 -15 years were included. Over the three year period, 39 children were included in the study, with a boy predominance (n=23, 59%). Among these children, 27 (66.7%) came from a social rehabilitation village for leprosy patients. One family member was affected by leprosy in 27 cases (69.2%). More than half of the children (23 cases, 58.9%) had multibacillary leprosy (lepromatous-lepromatous). All children underwent a 12-month treatment, at the end of which thirty-six (92.3%) children were healed. Leprosy is still present in Senegal despite the efforts made by the national programme to combat leprosy. In the light of these results, it is important to emphasize the role of active screening strategy targeted to children, which seems to have shown its effectiveness in the region. Early detection, contact tracing and early treatment are important factors in the reduction of the contagiousity of leprosy.

  11. Flood risk analysis and adaptive strategy in context of uncertainties: a case study of Nhieu Loc Thi Nghe Basin, Ho Chi Minh City

    Science.gov (United States)

    Ho, Long-Phi; Chau, Nguyen-Xuan-Quang; Nguyen, Hong-Quan

    2013-04-01

    The Nhieu Loc - Thi Nghe basin is the most important administrative and business area of Ho Chi Minh City. Due to system complexity of the basin such as the increasing trend of rainfall intensity, (tidal) water level and land subsidence, the simulation of hydrological, hydraulic variables for flooding prediction seems rather not adequate in practical projects. The basin is still highly vulnerable despite of multi-million USD investment for urban drainage improvement projects since the last decade. In this paper, an integrated system analysis in both spatial and temporal aspects based on statistical, GIS and modelling approaches has been conducted in order to: (1) Analyse risks before and after projects, (2) Foresee water-related risk under uncertainties of unfavourable driving factors and (3) Develop a sustainable flood risk management strategy for the basin. The results show that given the framework of risk analysis and adaptive strategy, certain urban developing plans in the basin must be carefully revised and/or checked in order to reduce the highly unexpected loss in the future

  12. [Care for elderly patients in Africa: Analysis of the financial implications of the SESAME plan on the budget of the regional hospital center in Thies, Senegal].

    Science.gov (United States)

    Faye, A; Diousse, P; Seck, I; Diongue, M; Ndiaye, P; Diagne-Camara, M; Tal-Dia, A; Dia, La

    2010-04-01

    The SESAME plan has been implemented at the Thies Regional Hospital Center (TRHC) for one year. The purpose of this study was to analyze the financial implications of the plan on the hospital budget for the sustainability of care for persons aged 60 and over. This descriptive study included analysis of budget data from October 2006 to September 2007 plus information obtained by interviewing the accountant and head of SESAME plan. The number of patients managed, sources of CHRT funding, grants from various SESAME plan partners, and expenditures for each partner were determined. The weight of the SESAME plan in the CHRT operating budget was determined by calculating the ratio of the overall cost of care for elderly persons in relation to the hospital's revenues and SESAME grants. During the study period, the CHRT received a total of 17375 elderly persons including 89% with no pension or social security. The institute pension scheme (IPRES) covered 21% of the plan as compared to 79% for the state. Utilization plan grants in relation to funding source was 41% for IPRES and 124% for the State. The total cost of services provided to beneficiaries of the SESAME plan exceeded the aggregate amount by 26 083 847 CFA francs. The weight of the SESAME plan in the operating cost of the CHRT was 17%. Prefinancing a plan to cover elderly care in hospitals should be sufficient to prevent deficits from impacting negatively on the operating budget of the hospital.

  13. DNA barcoding for identification of 'Candidatus Phytoplasmas' using a fragment of the elongation factor Tu gene.

    Directory of Open Access Journals (Sweden)

    Olga Makarova

    Full Text Available BACKGROUND: Phytoplasmas are bacterial phytopathogens responsible for significant losses in agricultural production worldwide. Several molecular markers are available for identification of groups or strains of phytoplasmas. However, they often cannot be used for identification of phytoplasmas from different groups simultaneously or are too long for routine diagnostics. DNA barcoding recently emerged as a convenient tool for species identification. Here, the development of a universal DNA barcode based on the elongation factor Tu (tuf gene for phytoplasma identification is reported. METHODOLOGY/PRINCIPAL FINDINGS: We designed a new set of primers and amplified a 420-444 bp fragment of tuf from all 91 phytoplasmas strains tested (16S rRNA groups -I through -VII, -IX through -XII, -XV, and -XX. Comparison of NJ trees constructed from the tuf barcode and a 1.2 kbp fragment of the 16S ribosomal gene revealed that the tuf tree is highly congruent with the 16S rRNA tree and had higher inter- and intra- group sequence divergence. Mean K2P inter-/intra- group divergences of the tuf barcode did not overlap and had approximately one order of magnitude difference for most groups, suggesting the presence of a DNA barcoding gap. The use of the tuf barcode allowed separation of main ribosomal groups and most of their subgroups. Phytoplasma tuf barcodes were deposited in the NCBI GenBank and Q-bank databases. CONCLUSIONS/SIGNIFICANCE: This study demonstrates that DNA barcoding principles can be applied for identification of phytoplasmas. Our findings suggest that the tuf barcode performs as well or better than a 1.2 kbp fragment of the 16S rRNA gene and thus provides an easy procedure for phytoplasma identification. The obtained sequences were used to create a publicly available reference database that can be used by plant health services and researchers for online phytoplasma identification.

  14. 单克隆抗体OX7的制备及大鼠抗Thy1系膜增生性肾炎模型的建立%Preparation of Monoclonal Antibody OX7 and Establishment of Anti-thy 1 Mesangiai Proliferative Nephritis Model in Rats

    Institute of Scientific and Technical Information of China (English)

    张术佳; 吕杨; 谢院生; 解汝娟; 师锁柱; 马强; 殷敏; 陈香美

    2009-01-01

    目的:制备单克隆抗体OX7(monoclonal antibody OX7,mAb OX7)对建立大鼠抗Thy1系膜增生性肾炎模型.方法:弗氏不完全佐剂预免疫Balb/c小鼠,腹腔注射OX7细胞,Protein A亲和层析法纯化腹水.用SDS-PAGE鉴定纯化后抗体的纯度.将所得抗体尾静脉注射Wistar大鼠,PAS染色观察正常对照组注射前及注射后第3天、第7天大鼠肾脏病理组织学改变.结果:SDS-PAGE电泳显示,纯化的抗体有IgG的轻链和重链两条带,无其他杂带,抗体浓度为2.06 mg/ml.PAS染色显示正常对照组肾小球结构正常,毛细血管袢开放良好;模型组第3天开始出现大量系膜细胞破坏,系膜基质溶解,肾小球毛细血管扩张;第7天系膜细胞重度增生.结论:通过将OX7细胞注入小鼠腹腔并用ProteinA亲和层析法纯化腹水,可成功制备高效价、高纯度及特异性强的mAb OX7,该抗体可成功建立抗Thy1系膜增生性肾炎动物模型.

  15. Civil Engineering Applications of Ground Penetrating Radar: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2013-04-01

    can be used by GPR operators to identify the signatures generated by uncommon targets or by composite structures. Repeated evaluations of the electromagnetic field scattered by known targets can be performed by a forward solver, in order to estimate - through comparison with measured data - the physics and geometry of the region investigated by the GPR. It is possible to identify three main areas, in the GPR field, that have to be addressed in order to promote the use of this technology in the civil engineering. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by Lara Pajewski, "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE, EL, ES, FI, FR, HR, IT, NL, NO, PL, PT, TR, UK), as well as Australia and U.S.A. The project will be developed within the frame of a unique approach based on the integrated contribution of University researchers, software developers, geophysics experts, Non-Destructive Testing equipment designers and producers, end users from private companies and public agencies. The main objective of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst promoting the effective use of this safe and non-destructive technique in the monitoring of systems. In this interdisciplinary Action, advantages and limitations of GPR will be highlighted, leading to the identification of gaps in knowledge and technology

  16. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": ongoing research activities and third-year results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    This work aims at disseminating the ongoing research activities and third-year results of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar." About 350 experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Colombia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been recognised among the running Actions as "COST Success Story" ("The Cities of Tomorrow: The Challenges of Horizon 2020," September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of

  17. Effect of 1,25-dihydroxyvitamin D3 influence on expressions of Ki67 and mTOR in Thy-1 nephritis model of rat%1,25(OH)2D3对Thy-1肾炎大鼠Ki67、mTOR表达的影响

    Institute of Scientific and Technical Information of China (English)

    杨锐; 索洁; 李建锋; 王文政; 赵瑾; 陶林; 杨晓萍

    2015-01-01

    Objective To study the expressions of Ki67 and mTOR in Thy-1 nephritis model of rat who were given 1,25-dihydroxyvitamin D3[1,25(OH)2D3] and to explore its mechanism. Methods Healthy male SD rats (n=90) were random⁃ly divided into three groups: control group, model group, 1,25(OH)2D3 treatment group (n=30 in each group). Model group and 1,25(OH)2D3 treatment group were intravenously injected with anti-Thy1 monoclonal antibody once via tail vein while the control group were administrated with same volume of normal saline through the same route. 1,25(OH)2D3 were adminis⁃trated at 0.5μg per day intra-gastrically for consecutive 21 days in 1,25(OH)2D3 treatment group while equal volume of pea⁃nut oil were given in control group and model group. Six rats were randomly selected from each group and sacrificed at the 1st , 3rd , 7th , 14th and 21st after drug intervention. Twenty four hour urine sample were collected in each rat just before it was culled to detect 24-hour urinary protein excretion. Renal tissue samples were harvested and stained with hematoxylin&eo⁃sin (H&E) and PAS to determine the renal pathological variation and the expressions of mTOR and Ki67 were assessed by immunohistochemistry. Results Urine protein begin to be detected at the first day after model was established, peaked at the 3rd days then started dropping until the 14th day when urine sample turned to normal. Urine protein levels were lower in 1, 25(OH)2D3 treatment group at the 1st,3rd,7th day after model establishment than those in model group(P<0.05). Compared with model group, the pathological damage of renal tissue in 1,25(OH)2D3 treatment group were alleviated at the 3rd and 7th day after model establishment (P < 0.05). Expressions of Ki67 and mTOR in 1, 25(OH)2D3 treatment group were reduced compared with those in model group (P<0.05). Twenty four hour urinary protein and expressions of Ki67 and mTOR as well as renal pathological damage were all positively correlated with each

  18. Genetic dissection of the resistance to nine anthracnose races in the common bean differential cultivars MDRK and TU.

    Science.gov (United States)

    Campa, Ana; Giraldez, Ramón; Ferreira, Juan José

    2009-06-01

    Resistance to nine races of the pathogenic fungus Colletotrichum lindemuthianum, causal agent of anthracnose, was evaluated in F(3) families derived from the cross between the anthracnose differential bean cultivars TU (resistant to races, 3, 6, 7, 31, 38, 39, 102, and 449) and MDRK (resistant to races, 449, and 1545). Molecular marker analyses were carried out in the F(2) individuals in order to map and characterize the anthracnose resistance genes or gene clusters present in these two differential cultivars. The results of the combined segregation indicate that at least three independent loci conferring resistance to anthracnose are present in TU. One of them, corresponding to the previously described anthracnose resistance locus Co-5, is located in linkage group B7, and is formed by a cluster of different genes conferring specific resistance to races, 3, 6, 7, 31, 38, 39, 102, and 449. Evidence of intra-cluster recombination between these specific resistance genes was found. The second locus present in TU confers specific resistance to races 31 and 102, and the third locus confers specific resistance to race 102, the location of these two loci remains unknown. The resistance to race 1545 present in MDRK is due to two independent dominant genes. The results of the combined segregation of two F(4) families showing monogenic segregation for resistance to race 1545 indicates that one of these two genes is linked to marker OF10(530), located in linkage group B1, and corresponds to the previously described anthracnose resistance locus Co-1. The second gene conferring resistance to race 1545 in MDRK is linked to marker Pv-ctt001, located in linkage group B4, and corresponds to the Co-3/Co-9 cluster. The resistance to race 449 present in MDRK is conferred by a single gene, located in linkage group B4, probably included in the same Co-3/Co-9 cluster.

  19. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar:" ongoing research activities and mid-term results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2015-04-01

    This work aims at presenting the ongoing activities and mid-term results of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar.' Almost three hundreds experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been praised among the running Actions as 'COST Success Story' ('The Cities of Tomorrow: The Challenges of Horizon 2020,' September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects

  20. Overview and comparative study of GPR international standards and guidelines - COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Marciniak, Marian; Benedetto, Andrea; Tosti, Fabio

    2016-04-01

    Ground Penetrating Radar (GPR) can be effectively used for non-destructive testing of composite structures and diagnostics affecting the whole life-cycle of civil engineering works. Nevertheless, few recognised international standards exist in this field and inhomogeneous recommendations are present in different countries. Moreover, the levels of knowledge, awareness and experience regarding the use of GPR in civil engineering vary strongly across different European areas. The COST Action TU1208 is working hard on leveraging these differences, by sharing and disseminating knowledge and experience, as well as by developing guidelines and protocols for a safe and effective use of GPR in civil engineering. GPR users need to know which is the best way to conduct GPR measurements and what the quality level for the results should be. The TU1208 guidelines will ensure a higher efficiency and quality of GPR services and they will constitute a scientific basis for the introduction of European Standards on the application of GPR in civil engineering. The aim of this contribution is to present an in-depth overview and critical analysis of the existing GPR international and national standards and guidelines. The main documents considered in our work are listed and briefly described in the following. Three standards are provided by the American Society for Testing and Materials (ASTM), to guide the GPR use for subsurface investigation, evaluation of asphalt-covered concrete bridge decks, and determination of pavement-layer thickness: 1. ASTM D6432-11, Standard Guide for Using the Surface Ground Penetrating Radar Method for Subsurface Investigation, ASTM International, West Conshohocken, PA, 2011, www.astm.org, DOI: 10.1520/D6432-11. 2. ASTM D6087-08, Standard Test Method for Evaluating Asphalt-Covered Concrete Bridge Decks Using Ground Penetrating Radar, ASTM International, West Conshohocken, PA, 2008, www.astm.org, DOI: 10.1520/D6087-08. 3. ASTM D4748-10, Standard Test Method

  1. High contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed Multispectral Optoacoustic Tomography (tuMSOT)

    CERN Document Server

    Stiel, Andre C; Jiang, Yuanyuan; Ntziachristos, Vasilis; Razansky, Daniel; Westmeyer, Gil G

    2014-01-01

    Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically controlled photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high contrast molecular optoacoustic imaging.

  2. Control de criaderos de Aedes aegypticon el programa Recicla por tu bienestar en Mérida, México

    OpenAIRE

    Mario A. Barrera-Pérez; Norma Pavía-Ruz; Jorge Eduardo Mendoza-Mézquita; Nerio Torres-Arcila; Ramón Hernández-Hernández; Francisco Castro-Gamboa; Eduardo Geded-Moreno; Azael Cohuo-Rodríguez; Anuar Medina-Barreiro; Edgar Koyoc-Cardeña; Héctor Gómez-Dantés; Axel Kroeger; Gonzalo Vázquez-Prokopec; Pablo Manrique-Saide

    2015-01-01

    Objetivos. Determinar la importancia de los criaderos de Ae. aegypti en Mérida; evaluar el impacto del programa Recicla por tu bienestar (RxB) sobre la presencia/abundancia de éstos y la percepción de los habitantes. Material y métodos. S e calculó la importancia de los criaderos por su productividad pupal. Se realizaron muestreos pre y post RxB en colonias para cuantificar el total de recipientes/criaderos. Se aplicó una encuesta a participantes sobre la percepción sobre RxB en colonias sel...

  3. Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice.

    Science.gov (United States)

    Jiang, Fei; He, Jinyan; Navarro-Alvarez, Nalu; Xu, Jian; Li, Xia; Li, Peng; Wu, Wenxue

    2016-01-01

    Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70. The sera of both rEF-Tu and rHSP70 treated BALB/c mice demonstrated increased levels of IgG, IFN-γ, TNF-α, IL-12(p70), IL-4, IL-5 and IL-6. In addition, ELISPOT assay showed significant increase in IFN-γ+ secreting lymphocytes in the rHSP70 group when compared to other groups. Collectively our study reveals that rHSP70 induces a significantly better cellular immune response in mice, and may act as a Th1 cytokine-like adjuvant in immune response induction. Finally, growth inhibition test (GIT) of M. ovipneumoniae strain Y98 showed that sera from rHSP70 or rEF-Tu-immunized mice inhibited in vitro growth of M. ovipneumoniae. Our data strongly suggest that EF-Tu and HSP70 of M. ovipneumoniae are membrane-associated proteins capable of inducing antibody production, and cytokine secretion. Therefore, these two proteins may be potential candidates for vaccine development against M. ovipneumoniae infection in sheep.

  4. Identification, genotypic relation, and clinical features of colistin-resistant isolates of Acinetobacter genomic species 13BJ/14TU from bloodstreams of patients in a university hospital.

    Science.gov (United States)

    Lee, Seung Yeob; Shin, Jong Hee; Park, Kyung Hwa; Kim, Ju Hee; Shin, Myung Geun; Suh, Soon Pal; Ryang, Dong Wook; Kim, Soo Hyun

    2014-03-01

    Colistin resistance remains rare among clinical isolates of Acinetobacter species. We noted the emergence of colistin-resistant bloodstream isolates of the Acinetobacter genomic species (GS) 13BJ/14TU from patients at a university hospital between 2003 and 2011. We report here, for the first time, the microbiological and molecular characteristics of these isolates, with clinical features of Acinetobacter GS 13BJ/14TU bacteremia. All 11 available patient isolates were correctly identified as Acinetobacter GS 13BJ/14TU using partial rpoB gene sequencing but were misidentified using the phenotypic methods Vitek 2 (mostly as Acinetobacter baumannii), MicroScan (mostly as A. baumannii/Acinetobacter haemolyticus), and the API 20 NE system (all as A. haemolyticus). Most isolates were susceptible to commonly used antibiotics, including carbapenems, but all were resistant to colistin, for which it is unknown whether the resistance is acquired or intrinsic. However, the fact that none of the patients had a history of colistin therapy strongly suggests that Acinetobacter GS 13BJ/14TU is innately resistant to colistin. The phylogenetic tree of multilocus sequence typing (MLST) showed that all 11 isolates formed a separate cluster from other Acinetobacter species and yielded five sequence types. However, pulsed-field gel electrophoresis (PFGE) revealed 11 distinct patterns, suggesting that the bacteremia had occurred sporadically. Four patients showed persistent bacteremia (6 to 17 days), and all 11 patients had excellent outcomes with cleared bacteremia, suggesting that patients with Acinetobacter GS 13BJ/14TU-associated bacteremia show a favorable outcome. These results emphasize the importance of precise species identification, especially regarding colistin resistance in Acinetobacter species. In addition, MLST offers another approach to the identification of Acinetobacter GS 13BJ/14TU, whereas PFGE is useful for genotyping for this species.

  5. CD90/THY1 is over-expressed in prostate cancer-associated fibroblasts and could serve as a cancer biomarker

    Energy Technology Data Exchange (ETDEWEB)

    True, Lawrence D.; Zhang, Hui; Ye, Mingliang; Huang, Chung-Ying; Nelson, Peter S.; Von Haller, Priska D.; Tjoelker, Larry W.; Kim, Jong Seo; Qian, Weijun; Smith, Richard D.; Ellis, William J.; Liebeskind, Emily S.; Liu, Alvin Y.

    2010-10-01

    A by-product in the processing of prostate tissue for cell sorting by collagenase digestion is the media supernatant that remains after the cells are harvested. These supernatants contain proteins made by the cells within the tissue. Quantitative proteomic analysis of Nglycosylated proteins detected an increased amount of CD90/THY1 in cancer supernatants compared to non-cancer supernatants. Immunohistochemistry showed that in all carcinomas, regardless of Gleason grade, a layer of CD90-positive stromal fibroblastic cells, approximately 5-to-10 cells deep, was localized to tumor glands. In contrast, a no more than 1-cell wide girth of CD90-positive stromal cells was found around benign glands. The increased number of CD90-positive stromal cells in cancer correlated with overexpression of CD90 mRNA detected by gene expression analysis of stromal cells obtained by laser-capture microdissection. There is increasing evidence that cancer-associated stroma plays a role in both tumor progression and carcinogenesis. Most experiments to identify cancer biomarkers have focused on the cancer cells. CD90, being a marker for prostate cancer-associated stroma, might be a potential biomarker for this cancer. A non-invasive test could be provided by a urine test. Proteomic analysis of urine from patients with prostate cancer identified CD90; conversely, CD90 was not detected in the urine of post-prostatectomy patients. Furthermore, this urinary CD90 protein was a variant CD90 protein not known to be expressed by such cells as lymphocytes that express CD90. These CD90 results were obtained from ~90 cases consisting of proteomic analysis of tissue and urine, immunohistochemistry, Western blot analysis of tissue media, flow cytometry of cells from digested tissue, and reverse transcriptase polymerase chain reaction analysis of isolated stromal cells.

  6. Nondestructive tests for railway monitoring. European Experience in COST Action TU1208

    Science.gov (United States)

    Fontul, Simona; Solla, Mercedes; Loizos, Andreas

    2016-04-01

    The railway monitoring is an important issue for a proper maintenance planning. With the increase in loads and travel speed, it is important to be able to diagnose the track defects and to plan the proper maintenance without interfering with the users. Traditionally, the maintenance actions are planned based on the geometric level parameters assessed without contact with the line, at traffic speed, by dedicated inspection vehicles. Nevertheless, the geometric condition of the line does not provide information on the defects causes. In order to complements the information on the causes, geophysics measurements can be performed in a nondestructive way. Among these later methods, Ground Penetrating Radar (GPR) is a quick and effective technique to evaluate infrastructure condition in a continuous manner, replacing or reducing the use of traditional drilling method. GPR application to railways infrastructures, during construction and monitoring phase, is relatively recent. It is based on the measuring of layers thicknesses and detection of structural changes. It also enables the assessment of materials properties that constitute the infrastructure and the evaluation of the different types of defects such as ballast pockets, fouled ballast, poor drainage, subgrade settlement and transitions problems. These deteriorations are generally the causes of vertical deviations in track geometry. Moreover, the development of new GPR systems with higher antenna frequencies, better data acquisition systems, more user friendly software and new algorithms for calculation of materials properties can lead to a regular use of GPR. A resume of the European experience in COST Action TU1208 of the application of GPR for railway monitoring and the measurement interpretation is presented in this paper. Also complementary nondestructive tests and other geophysical methods are referred, together with case studies of their application. The main troubleshooting and the needs for data analysis

  7. Reducing ppGpp level rescues an extreme growth defect caused by mutant EF-Tu.

    Science.gov (United States)

    Bergman, Jessica M; Hammarlöf, Disa L; Hughes, Diarmaid

    2014-01-01

    Transcription and translation of mRNA's are coordinated processes in bacteria. We have previously shown that a mutant form of EF-Tu (Gln125Arg) in Salmonella Typhimurium with a reduced affinity for aa-tRNA, causes ribosome pausing, resulting in an increased rate of RNase E-mediated mRNA cleavage, causing extremely slow growth, even on rich medium. The slow growth phenotype is reversed by mutations that reduce RNase E activity. Here we asked whether the slow growth phenotype could be reversed by overexpression of a wild-type gene. We identified spoT (encoding ppGpp synthetase/hydrolase) as a gene that partially reversed the slow growth rate when overexpressed. We found that the slow-growing mutant had an abnormally high basal level of ppGpp that was reduced when spoT was overexpressed. Inactivating relA (encoding the ribosome-associated ppGpp synthetase) also reduced ppGpp levels and significantly increased growth rate. Because RelA responds specifically to deacylated tRNA in the ribosomal A-site this suggested that the tuf mutant had an increased level of deacylated tRNA relative to the wild-type. To test this hypothesis we measured the relative acylation levels of 4 families of tRNAs and found that proline isoacceptors were acylated at a lower level in the mutant strain relative to the wild-type. In addition, the level of the proS tRNA synthetase mRNA was significantly lower in the mutant strain. We suggest that an increased level of deacylated tRNA in the mutant strain stimulates RelA-mediated ppGpp production, causing changes in transcription pattern that are inappropriate for rich media conditions, and contributing to slow growth rate. Reducing ppGpp levels, by altering the activity of either SpoT or RelA, removes one cause of the slow growth and reveals the interconnectedness of intracellular regulatory mechanisms.

  8. Reducing ppGpp level rescues an extreme growth defect caused by mutant EF-Tu.

    Directory of Open Access Journals (Sweden)

    Jessica M Bergman

    Full Text Available Transcription and translation of mRNA's are coordinated processes in bacteria. We have previously shown that a mutant form of EF-Tu (Gln125Arg in Salmonella Typhimurium with a reduced affinity for aa-tRNA, causes ribosome pausing, resulting in an increased rate of RNase E-mediated mRNA cleavage, causing extremely slow growth, even on rich medium. The slow growth phenotype is reversed by mutations that reduce RNase E activity. Here we asked whether the slow growth phenotype could be reversed by overexpression of a wild-type gene. We identified spoT (encoding ppGpp synthetase/hydrolase as a gene that partially reversed the slow growth rate when overexpressed. We found that the slow-growing mutant had an abnormally high basal level of ppGpp that was reduced when spoT was overexpressed. Inactivating relA (encoding the ribosome-associated ppGpp synthetase also reduced ppGpp levels and significantly increased growth rate. Because RelA responds specifically to deacylated tRNA in the ribosomal A-site this suggested that the tuf mutant had an increased level of deacylated tRNA relative to the wild-type. To test this hypothesis we measured the relative acylation levels of 4 families of tRNAs and found that proline isoacceptors were acylated at a lower level in the mutant strain relative to the wild-type. In addition, the level of the proS tRNA synthetase mRNA was significantly lower in the mutant strain. We suggest that an increased level of deacylated tRNA in the mutant strain stimulates RelA-mediated ppGpp production, causing changes in transcription pattern that are inappropriate for rich media conditions, and contributing to slow growth rate. Reducing ppGpp levels, by altering the activity of either SpoT or RelA, removes one cause of the slow growth and reveals the interconnectedness of intracellular regulatory mechanisms.

  9. The Dynamic Atmospheres of Mira Stars: Comparing the CODEX Models to PTI Time Series Observations of TU Andromedae

    Science.gov (United States)

    Hillen, M.; Verhoelst, T.; Degroote, P.; Acke, B.; Van Winckel, H.

    2015-08-01

    We present our already-published evaluation of the effectiveness of the CODEX models, released in 2011, in representing the atmospheres of M-type Mira variables. We present a high-precision interferometric K-band time series of TU And, consisting of 50 nights that cover eight consecutive pulsation cycles. At each phase, the flux at 2.2μm was obtained, along with the spectral shape and visibility points in five channels across the K band. We show a comparison between these data and the dynamical self-excited CODEX model which gives the closest match in stellar parameters yet available. Both the spectrum and the visibilities are consistently reproduced around visual minimum phases. Near the maximum phases, however, the current models predict a photosphere that is too hot and compact, surrounded by an extended atmosphere that lacks H2O opacity, compared to the observations. A better coverage in the model parameter space is needed to make firm conclusions as to the cause of the discrepancies. In the case of TU And, the discrepancy might be lifted by adopting a lower value of the mixing length parameter combined with an increased stellar mass and/or a decreased metallicity.

  10. Vysílač sériových dat ve formátu RS-232

    OpenAIRE

    Paul, Martin

    2008-01-01

    Cílem této bakalářské práce je navržení vysílače sériových dat formátu RS-232 ve vývojovém prostředí Xilinx ISE WebPACK s možností nastavit detaily formátu vysílaných dat vstupními signály. Dále je nutné prozkoumat požadavky navrženého vysílače na spotřebu strukturních prvků v různých cílových obvodech PLD a na závěr provést ověření funkčnosti návrhu pomocí vývojového kitu. The aim of my bachelor thesis is to create an RS-232 transmitter in the Xilinx ISE WebPACK development system with th...

  11. The Ca(2+)/Calmodulin/CaMKK2 Axis: Nature's Metabolic CaMshaft.

    Science.gov (United States)

    Marcelo, Kathrina L; Means, Anthony R; York, Brian

    2016-10-01

    Calcium (Ca(2+)) is an essential ligand that binds its primary intracellular receptor calmodulin (CaM) to trigger a variety of downstream processes and pathways. Central to the actions of Ca(2+)/CaM is the activation of a highly conserved Ca(2+)/CaM kinase (CaMK) cascade that amplifies Ca(2+) signals through a series of subsequent phosphorylation events. Proper regulation of Ca(2+) flux is necessary for whole-body metabolism and disruption of Ca(2+) homeostasis has been linked to various metabolic diseases. Here we provide a synthesis of recent advances that highlight the roles of the Ca(2+)/CaMK axis in key metabolic tissues. An appreciation of this information is critical to understanding the mechanisms by which Ca(2+)/CaM-dependent signaling contributes to metabolic homeostasis and disease.

  12. COST Action TU1208 - Working Group 4 - Combined use of GPR and other NDT methods & GPR applications in geosciences

    Science.gov (United States)

    Pajewski, Lara; Solla, Mercedes; Fontul, Simona

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 4 "Different applications of GPR and other NDT technologies in civil engineering" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action TU1208, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. WG4 deals with the use of GPR outside from the civil engineering area, namely in archaeological prospecting and cultural heritage diagnostics, agriculture and management of water resources, investigation of polluted industrial sites, non-destructive testing of living tree trunks, planetary exploration, demining, localization of people buried under avalanches and debris, and more. Furthermore, this WG studies the integration of GPR with other Non-Destructive Testing (NDT) methods. The most relevant achievements stemming from WG4 will be presented during the 2017 EGU GA. These are: (i) The collection of thorough information on the state-of-the-art, ongoing studies, problems and future research needs on the topics of interest for this WG; (ii) The performance of a plethora of interesting case studies in important sites all over Europe, including well-known historical places such as Stonehenge (United Kingdom), Carnuntum (Austria), the Wawel Cathedral (Cracow, Poland), the Tholos Tomb of Acharnon (Athens, Greece), the Łazienki Royal Palace (Warsaw, Poland), and more; (iii) WG4 contributed to the TU1208 Education Pack, an open educational package conceived to teach GPR in University

  13. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": first-year activities and results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2014-05-01

    This work aims at presenting the first-year activities and results of COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar". This Action was launched in April 2013 and will last four years. The principal aim of COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects, shape-reconstruction and estimation of geophysical parameters useful for civil engineering needs; (iv) networking for the design, realization and optimization of innovative GPR equipment; (v) comparing GPR with different NDT techniques, such as ultrasonic, radiographic, liquid-penetrant, magnetic-particle, acoustic-emission and eddy-current testing; (vi) comparing GPR technology and methodology used in civil engineering with those used in other fields; (vii) promotion of a more widespread, advanced and efficient use of GPR in civil engineering; and (viii) organization of a high-level modular training program for GPR European users. Four Working Groups (WGs) carry out the research activities. The first WG

  14. Moisture evaluation of wood material using GPR with WARR method - COST Action TU1208

    Science.gov (United States)

    Reci, Hamza; Sbart'i, Zoubir Mehdi; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work deals with the study of the sensitivity of GPR electromagnetic waves to moisture variation in wood material in relation with the direction of fibers and polarization of Electromagnetic field. The relations between relative permittivity and moisture content and the amplitude attenuation with distance was a target study using the direct waves in Wide Angle Radar Reflection (WARR) configuration. Comparison of results measured with reflected waves and direct waves was of main importance since they have different behavior in relation with moisture variation, due to different path of propagation. This research activity has been carried out during one Short-Term Scientific Missions (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November-December 2015. In context of durability evaluation of construction materials, several studies have been carried out by the I2M team, University of Bordeaux, using direct and reflected waves for the evaluation of water content on concrete and wood materials [1-3]. As related to the wood material there is one study carried out using the reflected waves on wood for different humidity and different wood samples, in all the direction of polarization using GPR technique ground coupled antenna at 1.5 GHz [3]. This work continued with different moisture content in order to study the behavior of direct waves as function of moisture. Results taken from those measurements are compared with them from Fixed Offset (reflected method) with one antenna (1.5GHz or 2.6GHz), realized from the previous studies from the I2M and already published [1-3]. The results taken from this work from the reflected waves, show that the effect of wood anisotropy is significant on the variation of relative permittivity with moisture content on wood sample and that is in good agreement with the previous results [3-6]. As related to the direct waves, a small

  15. Assessment of waterfront location in hardened concrete by GPR within COST Action TU1208

    Science.gov (United States)

    Rodríguez-Abad, Isabel; Klysz, Gilles; Balayssac, Jean Paul; Pajewski, Lara

    2016-04-01

    This work focuses on the analysis of the capability of Ground-Penetrating radar (GPR) technique for evaluating how the water penetrates into concrete samples by means of the assessment of the waterfront advance. Research activities have been carried out during a Short-Term Scientific Missions (STSMs) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November 2015. The evaluation of water penetrability is crucial in most building materials, such us concrete, since, water and aggressive chemical agents dissolved therein contribute to the deterioration of the material. A number of techniques have been developed to measure their advance in concrete. Although the most common method for measuring water content is the gravimetric method by observing the change in mass, this method has a large number of disadvantages. In this context, non-destructive techniques as GPR play an interesting role. In particular, the application of GPR in the building materials area is providing very promising and interesting results regarding the building materials characterization and especially concrete deterioration evaluation [1-3]. In addition, recent experimental studies highlight the strong relation between wave propagation parameters (velocity and energy level) and water content advance [4-5]. Water content has a decisive influence on dielectric properties and those might be assessed by the study of the wave properties that are derived by using GPR. Therefore, the waterfront advance will result in a change on wave parameters. In line with this, this research is focused on the development of specific processing algorithms necessary to understand how the water penetrates and how the wave parameters will be affected regarding the location of the antenna in reference to the water absorption direction. For this purpose, concrete samples were manufactured, which after curing (90 days) and oven

  16. Keeleuuendus ja tõlkimine Noor-Eesti kultuurilise utoopia raames : Villem Ridala tõlgitud "Süütu" näitel / Daniele Monticelli

    Index Scriptorium Estoniae

    Monticelli, Daniele

    2006-01-01

    Keeleuuenduse ja tõlketegevuse mõningastest olulistest seostest Noor-Eesti liikumisega alanud kultuurilise revolutsiooni kontekstis. Lähema lingvistilise vaatluse alla võetakse Gabriele D'Annunzio "L'Innocente" ("Süütu") Villem Ridala tõlkes. Järgneb: Keel ja Kirjandus, 2006, nr. 6, lk. 477-490

  17. Effect of thiostrepton and 3'-terminal fragments of aminoacyl-tRNA on EF-Tu and ribosome-dependent GTP hydrolysis.

    Science.gov (United States)

    Bhuta, P; Chládek, S

    1982-08-30

    The effect of the antibiotics thiostrepton and micrococcin on EF-Tu-catalyzed (ribosome-dependent) GTP hydrolysis in the presence of A-Phe, C-A-Phe, or C-C-A-Phe (related to the sequence of the 3'-terminus of aminoacyl-tRNA)(System I) or by methanol ('uncoupled GTPase', System II) was investigated. In System I, thiostrepton increases the binding affinities of the effectors to the EF-Tu.GTP.70 S ribosome complex, as well as the extent of the GTP hydrolysis, while the KmGTP is virtually unchanged. Similarly, in the uncoupled system (System II) and in the absence of effectors, thiostrepton significantly increases VmaxGTP, whereas KmGTP remains unaffected. Micrococcin is without any effect in both systems. The 'uncoupled GTPase' (in System II) is also strongly inhibited by C-A-Phe. The results indicate the crucial role of the EF-Tu site which binds the aminoacylated C-C-A terminus of aminoacyl-tRNA in promoting GTP hydrolysis. It follows that the binding of the model effectors (such as C-C-A-Phe) to that site is favorably influenced by the interaction of thiostrepton with the 50 S ribosomal subunit, whereas thiostrepton, per se, does not influence the affinity of EF-Tu for GTP.

  18. Institute of place making: A project by the chair of Landscape Architecture at the TU Delft. Oerol 2013: Sense of place

    NARCIS (Netherlands)

    Pouderoijen, M.T.; Piccinini, D.

    2013-01-01

    This booklet shows the results of a project developed by TU Delft in a Master elective course offered by the chair of Landscape Architecture: Landscape Architecture ON site, being part of Oerol Festival 2013. The aim of the project was to express the landscape of Terschelling into a temporarily proj

  19. El uso de drogas en acuacultura: ¿te dispones a aplicar una droga y/o químico en tu sistema de cultivo?

    OpenAIRE

    Montoya, Nelson

    2002-01-01

    El uso de drogas en acuacultura: ¿te dispones a aplicar una droga y/o químico en tu sistema de cultivo?. La aplicación de drogas no reguladas o no aprobadas para su uso en acuacultura representa un riesgo humano potencial.

  20. :Examination of Sulfate production by CB05TU, RACM2 & RACM2 with SCI initiated SO2,oxidation in the Northern Hemisphere"

    Science.gov (United States)

    We employ the Community Multiscale Air Quality model to examine tropospheric sulfateproduction in the northern hemisphere using the Carbon Bond 2005 chemical mechanism withupdated toluene chemistry (CB05TU) and the Regional Atmospheric Chemistry Mechanism(RACM2) without and with ...

  1. Investigation of functional aspects of the N-terminal region of elongation factor Tu from Escherichia coli using a protein engineering approach

    DEFF Research Database (Denmark)

    Laurberg, M; Mansilla, Francisco; Clark, Brian F. C.

    1998-01-01

    The function of the N-terminal region of elongation factor Tu is still unexplained. Until recently, it has not been visible in electron density maps from x-ray crystallography studies, but the presence of several well conserved basic residues suggest that this part of the molecule is of structura...

  2. 地球信息图谱与数字地球%On Geo-informatic TuPu and Digital Earth

    Institute of Scientific and Technical Information of China (English)

    廖克; 秦建新; 张青年

    2001-01-01

    Digital Earth can be understood as digitized reoresentation and the recognition for real earth and related, geo-phenomena. Digital Earth consists of spatial information with different levels and application models with different objectives. It includes the establishment of base and thematic digital maps with global, national, regional and urban scales, and satellite image data with different resolution and various geo-informations such as population, economy, society and their application systems.   Geo-informatic TuPu, as graphic representation of spatio-temporal change of geo-information, is an effective tool for the application of Digital Earth. “Tu”, in Chinese word, includes graphics, maps and images. The Chinese word “Pu” means the well-regulated sequence arrangement of the congener things, such as spectrum, chromatogram. “TuPu” involves the characteristics of “Tu” and “Pu” at the same time.   Geo-information TuPu can not only reflect spatial distribution characteristics of geo-phenomena and regional differences between them, but also reveal the deeper level rules related to the cause of formation, the process of evolution and morphology. Having the aid of computer visualization and Virtual Reality technology, TuPu can dynamically and muti-dimensionally reveal the spatial-temporal distribution and evolvement laws of all kinds of geo-phenomena.   In this paper, the technological system of Digital Earth and its significance, and the concept of Geo-informatic TuPu are discussed firstly, then, the model of Geo-informatic TuPu transmission is presented. On the basis of the above-mentioned researches, the relationship between Digital Earth and Geo-informatic TuPu is discussed. It reaches to the conclusion that the development of Digital Earth will provide with abundant information resources and strong technological support for Geo-informatic TuPu researches, and the enforcement of Digital Earth strategy will supply Geo-informatic Tu

  3. Two-way WKB Approximation Applied to GPR - COST Action TU1208

    Science.gov (United States)

    Prokopovich, Igor; Popov, Alexei; Marciniak, Marian; Pajewski, Lara

    2016-04-01

    account. In this work we study bistatic EM pulse probing of a horizontally layered medium in a 2D case. Coupled WKB equations set describing both forward and backward waves are derived and solved analytically. The comparison of our semi-analytical solutions with numerical calculations by gprMax software [4] demonstrates a good agreement, being hundreds of times faster than the letter. Our numerical results explain the protracted return pulses in the low-frequency GPR data. As an example, we discuss the experimental data obtained during the GPR mission in search of a big fragment of Chelyabinsk meteorite under a thick silt layer at the bottom of Chebarcul' Lake [5]. Acknowledgement The Authors are grateful to the European Cooperation in Science and Technology (www.cost.eu) facilitating this work by a Short-Term Scientific Missions (STSM) within the framework of the Action TU1208 "Civil engineering applications of Ground Penetrating Radar" (www.GPRadar.eu). References 1. H. Bremmer "Propagation of electromagnetic waves", in Handbuch der Physik, S. Flugge, Ed. Berlin-Goettingen-Heidelberg: Springer, 1958, pp. 423-639 2. L.M. Brekhovskikh, Waves in Stratified Media (in Russian). Moscow: USSR Academy of Sciences, 1957. 3. V.A.Vinogradov, V.V. Kopeikin, A.V. Popov, "An Approximate Solution of 1D Inverse Problem", in Proc. 10th Internat. Conf. on GPR, 21-24 June, 2004, Delft, The Netherlands 4. A. Giannopoulos, "Modelling ground penetrating radar by GprMax", Construction and Building Materials, vol. 19, no. 10, pp. 755-762, 2005, doi: 10.1016/j.conbuildmat.2005.06.007 5. V. V. Kopeikin , V. D. Kuznetsov, P. A. Morozov, A. V. Popov et al., "Ground penetrating radar investigation of the supposed fall site of a fragment of the Chelyabinsk meteorite in Lake Chebarkul'", Geochemistry International, vol. 51, no. 7, pp. 575-582, 2013, doi: 10.1134/S0016702913070112

  4. Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.

    Science.gov (United States)

    Kim, Jinhee; Kang, Won-Hee; Hwang, Jeena; Yang, Hee-Bum; Dosun, Kim; Oh, Chang-Sik; Kang, Byoung-Cheorl

    2014-08-01

    The protein-protein interaction between VPg (viral protein genome-linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad-spectrum resistance in Chinese cabbage (Brassica rapa) to Turnip mosaic virus (TuMV), which is one of the most important potyviruses, by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and B. rapa eIF(iso)4E. The seven amino acids in the cap-binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap-binding pockets, and mutated. Yeast two-hybrid assay and co-immunoprecipitation analysis demonstrated that W95L, K150L and W95L/K150E amino acid mutations of B. rapa eIF(iso)4E interrupted its interaction with TuMV VPg. All eIF(iso)4E mutants were able to complement an eIF4E-knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L, W95L/K150E and eIF(iso)4E wild-type were over-expressed in a susceptible Chinese cabbage cultivar. Evaluation of the TuMV resistance of T1 and T2 transformants demonstrated that the over-expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge-based approaches for the engineering of broad-spectrum resistance in Chinese cabbage.

  5. TU-100 (Daikenchuto and ginger ameliorate anti-CD3 antibody induced T cell-mediated murine enteritis: microbe-independent effects involving Akt and NF-κB suppression.

    Directory of Open Access Journals (Sweden)

    Nobuhiro Ueno

    Full Text Available The Japanese traditional medicine daikenchuto (TU-100 has anti-inflammatory activities, but the mechanisms remain incompletely understood. TU-100 includes ginger, ginseng, and Japanese pepper, each component possessing bioactive properties. The effects of TU-100 and individual components were investigated in a model of intestinal T lymphocyte activation using anti-CD3 antibody. To determine contribution of intestinal bacteria, specific pathogen free (SPF and germ free (GF mice were used. TU-100 or its components were delivered by diet or by gavage. Anti-CD3 antibody increased jejunal accumulation of fluid, increased TNFα, and induced intestinal epithelial apoptosis in both SPF and GF mice, which was blocked by either TU-100 or ginger, but not by ginseng or Japanese pepper. TU-100 and ginger also blocked anti-CD3-stimulated Akt and NF-κB activation. A co-culture system of colonic Caco2BBE and Jurkat-1 cells was used to examine T-lymphocyte/epithelial cells interactions. Jurkat-1 cells were stimulated with anti-CD3 to produce TNFα that activates epithelial cell NF-κB. TU-100 and ginger blocked anti-CD3 antibody activation of Akt in Jurkat cells, decreasing their TNFα production. Additionally, TU-100 and ginger alone blocked direct TNFα stimulation of Caco2BBE cells and decreased activation of caspase-3 and polyADP ribose. The present studies demonstrate a new anti-inflammatory action of TU-100 that is microbe-independent and due to its ginger component.

  6. 3TU.Datacentrum serves data-curation needs of geodesy, hydrology, and climate science in the Netherlands

    Science.gov (United States)

    Princic, A.; Rombouts, J. P.; de Smaele, M. M. P.

    2012-04-01

    3TU.Datacentrum (datacentrum.3tu.nl) is a portal for science- and technology research data in the Netherlands. It serves data-curation needs of scientists in different disciplines of engineering and science varying from hydrology, water management, electron microscopy, to remote sensing. This data center provides long-term archive and permanent access to research data, it enables sharing and reuse of research data, and provides tools for citation of research datasets. The versatility of disciplines and versatility of needs is a challenge of the data center. The solutions offered differ in the functionality offered to the user. For a complex collection of simple datasets consisting of ongoing hydrological measurements from several project partners, using several instruments, on several locations, over a long period of time, there was a need for central storage in order to share the data and enable easy combination of data sets. The measurements are not repeatable and are also valuable for multidisciplinary research, which justified investment in the preservation and 'publishing' of the data. For a simple collection of complex datasets consisting of measurements of the meteorological radar, obtaining high resolution temporal and spatial precipitation data including those non-detectable by standard meteorological radar like drizzle, there was a need to store the data due to volume. These data come from a single instrument and from a single organization. Sharing with other scientists was difficult, which justified investment in the preservation and 'publishing' of the data. 3TU.Datacentrum offered solutions for both cases. Standardized solution to both collections is the data model: datasets, instruments, locations and time frames are all objects on their own, with own metadata and interconnected through rdf relationships. These relations are exploited in the user interface for navigation and inclusion, e.g. of instrument and location metadata in the display of

  7. COST Action TU1208 - Working Group 2 - GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Derobert, Xavier; Fontul, Simona; Govedarica, Miro; Gregoire, Colette; Loizos, Andreas; Perez-Gracia, Vega; Plati, Christina; Ristic, Aleksandar; Tosti, Fabio; Van Geem, Carl

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 2 "GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The principal goal of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG2 include: 1. The state of the art on the use of GPR in civil engineering was composed and open issues were identified. The few existing international/national guidelines/protocols for GPR inspection in civil engineering were reviewed and discussed. Academic end-users, private companies and stakeholders presented their point of view and needs. 2. Guidelines for investigating flexible pavement by using GPR were prepared, with particular regard to layer-thickness assessment, moisture-content sensing, pavement-damage detection and classification, and other main GPR-based investigations in pavement engineering. 3. Guidelines for GPR sensing and mapping of underground utilities and voids were prepared, with a main focus on urban areas. 4. Guidelines for GPR assessment of concrete structures, with particular regard to inspections in bridges and tunnels, were prepared. 5. A report was composed, including a series of practical suggestions and very useful information to guide GPR users during building inspection. 6. WG2 Members carried out a plethora of case studies where GPR was used to survey roads, highways, airport runways, car

  8. The dynamic atmospheres of Mira stars: comparing the CODEX models to PTI time series of TU Andromedae

    Science.gov (United States)

    Hillen, M.; Verhoelst, T.; Degroote, P.; Acke, B.; van Winckel, H.

    2012-02-01

    Context. Our comprehension of stellar evolution on the AGB still faces many difficulties. To improve on this, a quantified understanding of large-amplitude pulsator atmospheres and interpretation in terms of their fundamental stellar parameters are essential. Aims: We wish to evaluate the effectiveness of the recently released CODEX dynamical model atmospheres in representing M-type Mira variables through a confrontation with the time-resolved spectro-photometric and interferometric PTI data set of TU And. Methods: We calibrated the interferometric K-band time series to high precision. This results in 50 nights of observations, covering 8 subsequent pulsation cycles. At each phase, the flux at 2.2 μm is obtained, along with the spectral shape and visibility points in 5 channels across the K-band. We compared the data set to the relevant dynamical, self-excited CODEX models. Results: Both spectrum and visibilities are consistently reproduced at visual minimum phases. Near maximum, our observations show that the current models predict a photosphere that is too compact and hot, and we find that the extended atmosphere lacks H2O opacity. Since coverage in model parameter space is currently poor, more models are needed to make firm conclusions on the cause of the discrepancies. We argue that for TU And, the discrepancy could be lifted by adopting a lower value of the mixing length parameter combined with an increase in the stellar mass and/or a decrease in metallicity, but this requires the release of an extended model grid. Figure 4 and Table 1 are available in electronic form at http://www.aanda.org

  9. 有关CA

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ 什么是CA? CA(Certificaton Authority)是认证机构的国际通称,是指对数字证书的申请者发放、管理、取消数字证书的机构.CA的作用是检查证书持有者身份的合法性,并签发证书(在证书上签字),以防证书被伪造或篡改.

  10. Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays: cDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities.

    Science.gov (United States)

    Rapala-Kozik, Maria; Olczak, Mariusz; Ostrowska, Katarzyna; Starosta, Agata; Kozik, Andrzej

    2007-12-01

    A thiamine biosynthesis gene, thi3, from maize Zea mays has been identified through cloning and sequencing of cDNA and heterologous overexpression of the encoded protein, THI3, in Escherichia coli. The recombinant THI3 protein was purified to homogeneity and shown to possess two essentially different enzymatic activities of HMP(-P) [4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate)] kinase and TMP (thiamine monophosphate) synthase. Both activities were characterized in terms of basic kinetic constants, with interesting findings that TMP synthase is uncompetitively inhibited by excess of one of the substrates [HMP-PP (HMP diphosphate)] and ATP. A bioinformatic analysis of the THI3 sequence suggested that these activities were located in two distinct, N-terminal kinase and C-terminal synthase, domains. Models of the overall folds of THI3 domains and the arrangements of active centre residues were obtained with the SWISS-MODEL protein modelling server, on the basis of the known three-dimensional structures of Salmonella enterica serotype Typhimurium HMP(-P) kinase and Bacillus subtilis TMP synthase. The essential roles of Gln98 and Met134 residues for HMP kinase activity and of Ser444 for TMP synthase activity were experimentally confirmed by site-directed mutagenesis.

  11. Topological organization of CA3-to-CA1 excitation.

    Science.gov (United States)

    Hongo, Yoshie; Ogawa, Koichi; Takahara, Yuji; Takasu, Keiko; Royer, Sebastien; Hasegawa, Minoru; Sakaguchi, Gaku; Ikegaya, Yuji

    2015-09-01

    The CA1-projecting axons of CA3 pyramidal cells, called Schaffer collaterals, constitute one of the major information flow routes in the hippocampal formation. Recent anatomical studies have revealed the non-random structural connectivity between CA3 and CA1, but little is known regarding the functional connectivity (i.e. how CA3 network activity is functionally transmitted downstream to the CA1 network). Using functional multi-neuron calcium imaging of rat hippocampal slices, we monitored the spatiotemporal patterns of spontaneous CA3 and CA1 burst activity under pharmacological GABAergic blockade. We found that spatially clustered CA3 activity patterns were transformed into layered CA1 activity sequences. Specifically, synchronized bursts initiated from multiple hot spots in CA3 ensembles, and CA1 neurons located deeper in the pyramidal cell layer were recruited during earlier phases of the burst events. The order of these sequential activations was maintained across the bursts, but the sequence velocity varied depending on the inter-burst intervals. Thus, CA3 axons innervate CA1 neurons in a highly topographical fashion.

  12. Biomarker CA125

    DEFF Research Database (Denmark)

    Kargo, Anette Stolberg

    be detected months before symptoms arise and recurrence is visible on imaging. Therefore, biochemical detection of potential relapse by CA125 assessment can cause significant distress. A decision aid (DA) is a tool that provides information and describes advantages and disadvantages of a specific intervention...

  13. A new interpretation of the deep-part of Senegal-Mauritanian Basin in the Diourbel-Thies area by integrating seismic, magnetic, gravimetric and borehole data: Implication for petroleum exploration

    Science.gov (United States)

    Ndiaye, Matar; Ngom, Papa Malick; Gorin, Georges; Villeneuve, Michel; Sartori, Mario; Medou, Joseph

    2016-09-01

    The Diourbel-Thies area is located in the centre of the onshore part of the Senegal-Mauritanian Basin (SMB). The new interpretation of old petroleum data (2-D seismic lines and drilling data of three oil wells) in the deeppart of this poorly evaluated zone, integrating gravimetric and magnetic data, has allowed the distinction of the Hercynian ante-rift phase (U1) which is distinguished from the syn-rift phase (U2) probably of Permo-Triassic to Middle Jurassic age. The syn-rift phase resulted in a series of compartments or grabens infilling aligned in a North-South direction. Tholeiitic volcanism of the Central Atlantic Magmatic Province (CAMP) is present in the syn-rift phase of the Diourbel-Thies area. The syn-rift deposits and associated volcanics allow us to surmise that the Diourbel basin represents a deeper rift basin. In comparison with other Central Atlantic Margins (CAM), the Diourbel rift basin could be one of the numerous rift basins that formed during the Permo-Triassic age. From a petroleum exploration perspective, the existence of the Diourbel rift basin is attractive because of the presence of structures that are excellent for deep gas exploration.

  14. Electrodeposition of Ca Metal in CaCl2-CaO Molten Salt

    Institute of Scientific and Technical Information of China (English)

    GUO; Jun-kang; WANG; Chang-shui; CAO; Long-hao; OUYANG; Ying-gen

    2013-01-01

    To realize the continuouscalciothermic reduction in molten salts,the electrodeposition behavior of Ca metal in CaCl2-CaO molten salt was investigated by cylic voltammetry.The cyclic voltammograms at the scan rate of 100 mV/s are shown in Fig.1.As is shown,the electrodeposition potential of Ca deviated from-1.66 V to-0.97 V after CaO was added to molten CaCl2 and the decomposition of CaO

  15. Effect of combined leflunomide and benazepril therapy on proteinuria and renal function in experimental progressive Thy-1 glomerulonephritis rats%来氟米特联合苯那普利对慢性抗thy1肾炎大鼠蛋白尿及肾功能的影响

    Institute of Scientific and Technical Information of China (English)

    张承英; 张建荣; 耿燕秋; 丁韬

    2012-01-01

    Objective To observe the effect of combined leflunomide and benazepril therapy on proteinuria and renal function in experimental progressive Thy - 1 glomerulonephritis rats. Methods After one - side renal ablation, the surviving male Wistar rats were randomly assigned to five groups; one - side renal ablation group, untreated group ( model group) , leflunomide treated group, benazepril treated group, and leflunomide plus benazepril treated group . 0X7(100 mg/kg)was injected into these groups except one side renal ablation group through the caudal vein three days after renal ablation, and then 0X7 antibody was injected once a week, for three times. Distilled water or medicine was administered respectively by gavage for a period of 12 weeks. The general status, body weight, proteinuria (UP) , serum creatinine(Scr) , glomerular and tubulointerstitial histological damage scores were also measured. Results At the end of 12 week treatment, leflunomide treated group, benazepril treated group and leflunomide plus benazepril treated group showed reduced 24 h UP, Scr and smaller scores of glomerular and tubulointerstitial histological damage compared with model group. Compared with leflunomide treated group and benazepril treated group, leflunomide plus benazepril treated group showed smaller 24h UP, Scr and scores of glomerular and tubulointerstitial histological damage. Conclusions Combined leflunomide and benazepril therapy reduces 24h UP and Scr in experimental progressive Thy - 1 glomerulonephritis rats and slows the progression of chronic renal injury.%目的 探讨来氟米特联合苯那普利对慢性抗thy1肾炎大鼠蛋白尿及肾功能的影响.方法 雄性Wistar大鼠行单侧肾切除后随机分为单纯肾切组、模型对照组、来氟米特治疗组、苯那普利治疗组及来氟米特联合苯那普利治疗组,每组12只,除单纯肾切组外,其余各组在单侧肾切除后3 d尾静脉注射OX7抗体(100 mg/kg),1周后尾静脉连续注射OX7

  16. TU-G-BRD-05: Results From Multi-Institutional Measurements with An Anthropomorphic Spine Phantom

    Energy Technology Data Exchange (ETDEWEB)

    Molineu, A; Hernandez, N; Alvarez, P; Followill, D [UT MD Anderson Cancer Center, Houston, TX (United States)

    2015-06-15

    Purpose: To analyze the results from an anthropomorphic spine phantom used for credentialing institutions for National Cancer Institute (NCI) sponsored clinical trial. Methods: An anthropomorphic phantom that contains left and right lungs, a heart, an esophagus, spinal cord, bony material and a PTV was sent to institutions wishing to be credentialed for NCI trials. The PTV holds 4 TLD and radiochromic film in the axial and sagittal planes. The heart holds one TLD. Institutions created IMRT plans to cover ≥90% of the PTV with 6 Gy and limit the cord dose to <0.35cc receiving 3.75 Gy and <1.2cc receiving 2.63 Gy. They were instructed to treat the phantom as they would a patient, including making plan specific IMRT/SBRT QA measurements before treatment. The TLD results in the PTV were required to be within ±7% of the plan dose. A gamma calculation was performed using the film results and the submitted DICOM plan. ≥85% of the analyzed region was required to pass a 5%/3 mm criteria. Results: 176 institutions irradiated the spine phantom for a total of 255 results. The pass rate was 73% (187 irradiations) overall. 44 irradiations failed only the gamma criteria, 2 failed only the dose criteria and 22 failed both. The most used planning systems were Eclipse (116) and Pinnacle (52) and they had pass rates of 76% and 71%, respectively. The AAA algorithm had a pass rate of 77% while superposition type algorithms had a 71% pass rate. The average TLD measurement to institution calculation ratio was 0.99 (0.04 std dev.). The average percent pixels passing the gamma criteria for films was 89% (12% std dev.) Conclusion: Results show that this phantom is an important part of credentialing and that we have room for improvement in IMRT/SBRT spine treatments. This work was supported by PHS CA180803 and CA037422 awarded by NCI, DHHS.

  17. tu ša šarri. La « loi du roi »dans la Babylonie achéménide et séleucide dātu ša šarri, The “Law of the King” in Achaemenid and Seleucid Babylonia

    Directory of Open Access Journals (Sweden)

    Sophie Démare-Lafont

    2009-06-01

    Full Text Available Cet article examine le champ sémantique de l’expression dātu ša šarri, « loi du roi », formée sur un terme d’origine vieux-perse (data. La dizaine d’occurrences d’époques achéménide et séleucide montre que la « loi du roi »désigne un mode d’élaboration de la norme issu de la pratique judiciaire du souverain, progressivement compilée pour former un corps de règles invoquées dans les contrats. Ce procédé de création de la loi et de fabrication des recueils législatifs rappelle celui du rescrit romain.This article investigates the meaning of the formula dātu ša šarri, “law of the king”, using the akkadian dātu based on the old Persian word dāta. The ten Achaemenid and Seleucid occurrences of this formula suggest a process of creation of legal rules comparable to the Roman rescript: the legal standard dātu ša šarri derives from the judicial practice of the king, whose decisions were progressively compiled in order to create a body of regulations which were invoked in contracts.

  18. Sulfide capacities of CaO-CaF2-CaCl2 melts

    Science.gov (United States)

    Simeonov, Simeon; Sakai, Toshihiko; Maeda, Masafumi

    1992-06-01

    The sulfide capacityC_{s^{2 - } } = ({text{pct S}}^{{text{2 - }}} )(p_{{text{O}}_{text{2}} } /p_{{text{S}}_{text{2}} } )^{1/2} ) of CaO-CaF2-CaCl2 slag was determined at temperatures from 1000 °C to 1300 °C by equilibrating molten slag, molten silver, and CO-CO2-Ar gas mixture. The sulfide capacity increases with replacing CaCl2 by CaF2 in slags of constant CaO contents. The sulfide capacity also increases with increasing temperature as well as with increasing CaO content at a constant ratio of CaF2/CaCl2 of unity. A linear relationship between the sulfide capacity and carbonate capacity in literature was observed on a logarithmic scale.

  19. COST Action TU1208 - Working Group 3 - Electromagnetic modelling, inversion, imaging and data-processing techniques for Ground Penetrating Radar

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Sesnic, Silvestar; Randazzo, Andrea; Lambot, Sébastien; Benedetto, Francesco; Economou, Nikos

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 3 "Electromagnetic methods for near-field scattering problems by buried structures; data processing techniques" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. Among the most interesting achievements of WG3, we wish to mention the following ones: (i) A new open-source version of the finite-difference time-domain simulator gprMax was developed and released. The new gprMax is written in Python and includes many advanced features such as anisotropic and dispersive-material modelling, building of realistic heterogeneous objects with rough surfaces, built-in libraries of antenna models, optimisation of parameters based on Taguchi's method - and more. (ii) A new freeware CAD was developed and released, for the construction of two-dimensional gprMax models. This tool also includes scripts easing the execution of gprMax on multi-core machines or network of computers and scripts for a basic plotting of gprMax results. (iii) A series of interesting freeware codes were developed will be released by the end of the Action, implementing differential and integral forward-scattering methods, for the solution of simple electromagnetic problems by buried objects. (iv) An open database of synthetic and experimental GPR radargrams was created, in cooperation with WG2. The idea behind this initiative is to give researchers the

  20. COST Action TU1208 - Working Group 1 - Design and realisation of Ground Penetrating Radar equipment for civil engineering applications

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; D'Amico, Sebastiano; Ferrara, Vincenzo; Frezza, Fabrizio; Persico, Raffaele; Tosti, Fabio

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 1 "Novel Ground Penetrating Radar instrumentation" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.cost.eu, www.GPRadar.eu). The principal goal of the Action, which started in April 2013 and is ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG1 include: 1. The state of the art on GPR systems and antennas was composed; merits and limits of current GPR systems in civil engineering applications were highlighted and open issues were identified. 2. The Action investigated the new challenge of inferring mechanical (strength and deformation) properties of flexible pavement from electromagnetic data. A semi-empirical method was developed by an Italian research team and tested over an Italian test site: a good agreement was found between the values measured by using a light falling weight deflectometer (LFWD) and the values estimated by using the proposed semi-empirical method, thereby showing great promises for large-scale mechanical inspections of pavements using GPR. Subsequently, the method was tested on a real scale, on an Italian road in the countryside: again, a good agreement between LFWD and GPR data was achieved. As a third step, the method was tested at larger scale, over three different road sections within the districts of Madrid and Guadalajara, in Spain: GPR surveys were carried out at the speed of traffic for a total of 39 kilometers, approximately; results were collected by using different GPR antennas

  1. Incidence and remission of parasomnias among adolescent children in the Tucson Children’s Assessment of Sleep Apnea (TuCASA) Study

    OpenAIRE

    Furet O; Goodwin JL; Quan SF

    2011-01-01

    Background: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group.Methods: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1) and subsequently re-studied them approximately 5 years later (Time 2). At both time points parents were asked to complete a comprehensive sleep habits questionnaire desi...

  2. Praćenje ostataka sulfonilurea u zemljištu u realnim uslovima primenom visoko-pritisne tečne hromatografije

    OpenAIRE

    Grahovac, Nada

    2016-01-01

    U okviru doktorske disertacije razvijena je brza i selektivna metoda zaodređivanje i praćenje ostataka herbicida na bazi sulfonilurea (nikosulfuron,oksasulfuron, tribenuron metil, tritosulfuron, triasulfuron, rimsulfuron iprosulfuron) u zemljištu primenom visoko pritisne tečne hromatografije saUV detektor sa nizom fotoosetljivih dioda (HPLC-UV-DAD). Razdvajanjesulfonilurea je izvedeno uz primenu Zorbax Eclipse XDB-C18 kolone. U ciljudefinisanja odgovarajućih uslova analize, ispitan je ...

  3. A great honor and a huge challenge for China: You-you TU getting the Nobel Prize in Physiology or Medicine.

    Science.gov (United States)

    Yuan, Da; Yang, Xue; Guo, Jun-Chao

    2016-05-01

    Public excitement over the award of the 2015 Nobel Prize in Physiology or Medicine to the Chinese medical scientist You-you TU for the discovery of a herbal anti-malarial, may mislead the Chinese people into believing that traditional Chinese herbal medi-cine can be used to cure all disease without any ad-verse effects. The aim of this paper is to explain the advantages and disadvantages of herbal traditional Chinese medicine (TCM) objectively.

  4. "Toi qui, de façon générale..." : propositions relatives et "tu" générique en samba leko

    OpenAIRE

    Fabre, Gwenaëlle

    2004-01-01

    A partir de récits techniques en samba leko, je cherche ici à rendre compte de constructions originales qui associent un "tu" ou un "nous" au personnage central de l'activité décrite. Sur le plan syntaxique, il s'agit d'énoncés complexes qui comportent une proposition principale, une proposition relative et un topique.

  5. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC

    Directory of Open Access Journals (Sweden)

    Richard F. Reschen

    2012-03-01

    Dgp71WD/Nedd1 proteins are essential for mitotic spindle formation. In human cells, Nedd1 targets γ-tubulin to both centrosomes and spindles, but in other organisms the function of Dgp71WD/Nedd1 is less clear. In Drosophila cells, Dgp71WD plays a major part in targeting γ-tubulin to spindles, but not centrosomes, while in Xenopus egg extracts, Nedd1 acts as a more general microtubule (MT organiser that can function independently of γ-tubulin. The interpretation of these studies, however, is complicated by the fact that some residual Dgp71WD/Nedd1 is likely present in the cells/extracts analysed. Here we generate a Dgp71WD null mutant lacking all but the last 12 nucleotides of coding sequence. The complete loss of Dgp71WD has no quantifiable effect on γ-tubulin or Centrosomin recruitment to the centrosome in larval brain cells. The recruitment of γ-tubulin to spindle MTs, however, is severely impaired, and spindle MT density is reduced in a manner that is indistinguishable from cells lacking Augmin or γ-TuRC function. In contrast, the absence of Dgp71WD leads to defects in the assembly of the acentrosomal female Meiosis I spindle that are more severe than those seen in Augmin or γ-TuRC mutants, indicating that Dgp71WD has additional functions that are independent of these complexes in oocytes. Moreover, the localisation of bicoid RNA during oogenesis, which requires γ-TuRC function, is unperturbed in Dgp71WD120 mutants. Thus, Dgp71WD is not simply a general cofactor required for γ-TuRC and/or Augmin targeting, and it appears to have a crucial role independent of these complexes in the acentrosomal Meiosis I spindle.

  6. VizieR Online Data Catalog: TU UMa light curves and maxima, CL Aur minima (Liska+, 2016)

    Science.gov (United States)

    Liska, J.; Skarka, M.; Mikulasek, Z.; Zejda, M.; Chrastina, M.

    2016-02-01

    Differential photometry for RR Lyrae star TU UMa in the 1st and 2nd file. The measurements were obtained using 24-inch and 1-inch telescopes, respectively. The observations were performed at the Masaryk University Observatory in Brno (3 nights, 24-inch), and at the private observatory in Brno (16 nights, 1-inch) in the Czech Republic from December 2013 to June 2014. Observing equipments consisted of 24-inch Newtonian telescope (600/2780mm, diameter/focal length) and a Moravian Instruments CCD camera G2-4000 with Stromgren photometric filters vby, and of 1-inch refractor (a photographic lens Sonnar 4/135mm, lens focal ratio/focal length) and ATIK 16IC CCD camera with green photometric filter with similar throughput as the Johnson V filter. Exposures were v - 60s, b - 30s, y - 30s, green - 30s. For the small aperture telescope, five frames were combined to a single image to achieve a better signal-to-noise ratio. The time resolution of a such combined frame is about 170s. CCD images were calibrated in a standard way (dark frame and flat field corrections). The C-Munipack software (Motl 2009) was used for this processing as well as for differential photometry. The comparison star BD+30 2165 was the same for both instruments, but the control stars were BD+30 2164 (for the 24-inch telescope) and HD 99593 (for the 1-inch telescope). The 3rd file contains maxima timings of TU UMa adopted from the GEOS RR Lyr database, from the latest publications, together with maxima timings determined in our study. Times of maxima were calculated from our observations, sky-surveys data (Hipparcos, NSVS, Pi of the Sky, SuperWASP), photographic measurements (project DASCH), and from several published datasets, in which the maxima were omitted or badly determined - Boenigk (1958AcA.....8...13B), Liakos, Niarchos (2011IBVS.6099....1L, 2011IBVS.5990....1L), Liu, Janes (1989ApJS...69..593L), Preston et al. (1961ApJ...133..484P). The 4th file contains minima timings of eclipsing binary CL Aur

  7. GPR applications in civil engineering collaborating with a Company and preparation of material for the educational package to teach in Universities. STSM, COST Action TU1208

    Science.gov (United States)

    Sossa, Viviana; Assunção, Sonia; Pérez, Vega

    2017-04-01

    The STSM, Short-Term Missions, was carried out with the support of Murphy Survey company from Dublin - Ireland, was proposed for to Develop a module "GPR Applications in Archaeology" of the educational pack defined in the different meetings of the Cost Action TU 1208. The employ of GPR is mainly determined by the limits and uncertainties associated to this technique that affects to the measurements and the results. Private companies and end-users are highly interested in known the limits associated to particular cases and the advantages of the methodology compared to other studies. During this STSM, we plan to prepare a field survey, analyzing the possible problems and defining the objectives, and to acquire data to assess the structure. This data will be analyzed and processed in order to determine the limits of the technique in the particular case. The results will be compared to other surveys, in order to determine some clear restrictions that the company need to take into account in further works. Aknowledgments: This work has been partially funded by the EU COST Action TU1208, "Civil Engineering Applications of Ground Penetrating Radar", and it is a contribution to the working group 2.2 tasks in the COST Action TU1208.

  8. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles.

    Science.gov (United States)

    Shiri, Mahdi; Navaei-Nigjeh, Mona; Baeeri, Maryam; Rahimifard, Mahban; Mahboudi, Hossein; Shahverdi, Ahmad Reza; Kebriaeezadeh, Abbas; Abdollahi, Mohammad

    Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells.

  9. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

    Science.gov (United States)

    Shiri, Mahdi; Navaei-Nigjeh, Mona; Baeeri, Maryam; Rahimifard, Mahban; Mahboudi, Hossein; Shahverdi, Ahmad Reza; Kebriaeezadeh, Abbas; Abdollahi, Mohammad

    2016-01-01

    Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells. PMID:27920530

  10. Burtnieku novada nekroģeogrāfija. Atvērto kapsētu veidošanas prakse

    OpenAIRE

    Seļicka, Anita

    2012-01-01

    Maģistra darba „Burtnieku novada nekroģeogrāfija. Atvērto kapsētu veidošanas prakse” mērķis ir izvērtēt Burtnieku novada aktīvo kapsētu veidošanas praksi nekroģeogrāfijas koncepciju un pieeju kontekstā. Pētījuma teorētiskajā daļā tiek apskatīts nekroģeogrāfijas koncepts, dažādās kapsētas jēdziena uztveres un duālismi tajā. Darbā analizēti piecu Burtnieku novada kapsētu telpiskie elementi, to struktūras veidošanās, tiesiskais regulējums, iesaistīto personu un apkārtējās teritorijas konteksts k...

  11. Pilnīgi un daļēji ar stronciju aizvietotu peroksiapatītu sintēze un to analīze

    OpenAIRE

    Valkovska, Valda

    2016-01-01

    Pilnīgi un daļēji ar stronciju aizvietotu peroksiapatītu sintēze un to analīze. Valkovska V., darba vadītāja docente, Dr. chem. Osīte A. Bakalaura darbs, 42 lappuses, 19 attēli, 4 tabulas, 46 literatūras avoti, 5 pielikumi. Latviešu valodā. Darbā ir apkopota informācija par hidroksilapatītu un peroksiapatītu uzbūvi, īpašībām, izmantošanu, iegūšanu un analīzes metodēm. Salīdzinātas atšķirīgu sintēžu priekšrocības un trūkumi. Eksperimentālajā daļā veiktas pilnīgi un daļēji ar stronciju aizvieto...

  12. TU-CD-303-05: Unveiling Tumor Heterogeneity by Molecular Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Jeraj, R. [University of Wisconsin (United States)

    2015-06-15

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166.

  13. TU-CD-BRB-12: Radiogenomics of MRI-Guided Prostate Cancer Biopsy Habitats

    Energy Technology Data Exchange (ETDEWEB)

    Stoyanova, R; Lynne, C; Abraham, S; Patel, M; Jorda, M; Kryvenko, O; Ishkanian, A; Abramowitz, M; Pollack, A [University of Miami, Miami, FL (United States); Tachar, M; Erho, N; Buerki, C; Lam, L; Davicioni, E [GenomeDx Biosciences Inc., Vancouver, British Columbia (Canada)

    2015-06-15

    identified, targeting with radiotherapy should improve outcome. This is the first demonstration of a link between quantitative imaging features (radiomics) with genomic features in MRI-directed prostate biopsies. The research was supported by NIH- NCI R01 CA 189295 and R01 CA 189295; E Davicioni is partial owner of GenomeDx Biosciences, Inc. M Takhar, N Erho, L Lam, C Buerki and E Davicioni are current employees at GenomeDx Biosciences, Inc.

  14. Los narûs (kudurrus babilónicos del Bronce Final y el Hierro = The Babylonian narû (kudurrētu in Late Bronze and Iron Age

    Directory of Open Access Journals (Sweden)

    Sara Arroyo Cuadra

    2016-11-01

    Full Text Available Los narû babilónicos, conocidos tradicionalmente con el nombre de kudurrētu (en singular kudurru, fueron unos monumentos de piedra que incluían representaciones divinas encarnadas generalmente por sus emblemas y escenas con figuras antropomorfas, junto a un texto administrativo sobre transacciones  de tierras. Estos narû vieron la luz con la Dinastía Casita de Babilonia y dejaron de utilizarse en el período Neo-babilónico (ca. 1400-650 a.C.. La gran mayoría de los ejemplares se ha descubierto en Susa en contextos arqueológicos descontextualizados, pero los ejemplares hallados in situ nos hablan de que su dispersión geográfica original hay que situarla en el territorio de la Baja Mesopotamia. Hemos emprendido una investigación orientada a estudiar el corpus de estos monumentos integrado hasta el momento por 178 ejemplares, adoptando una postura interdisciplinar que incluye el análisis de la información que transmiten sus textos, iconografía, materialidad y contexto arqueológico. Por ello, a lo largo del presente artículo se intentará definir el criterio de adscripción de los ejemplares conocidos a dicho corpus según sus propias inscripciones y los estudios previos de  otros autores. A continuación, se hablará de las circunstancias que pudieron motivar su aparición y de los eventos histórico-políticos que pudieron influir en su evolución, así como del ámbito geográfico en que estuvieron vigentes. Después, se analizará el contenido de sus inscripciones y la simbología de su iconografía para, aportar, finalmente, un estudio comparativo entre textos y símbolos, quedando todo ello plasmado en un apartado final de conclusiones.Babylonian narû, traditionally named kudurrētu, were stone monuments with divine representations (generally through its divine emblems and figurative scenes with anthropomorphic figures, together with an administrative text about a land grant. These narû originated in Kassite Babylon and

  15. FTY720 attenuates rat anti-Thy-1 mesangial proliferative glomerulonephritis by inhibition of transforming growth factor βl-connective tissue growth factor pathway%转化生长因子β1-结缔组织生长因子途径在FTY720干预大鼠抗Thy-1系膜增生性肾炎中的作用

    Institute of Scientific and Technical Information of China (English)

    江警予; 黄晓东; 王奕; 邓艾平; 周建华

    2012-01-01

    目的 观察并探讨FTY720是否通过抑制转化生长因子β1-结缔组织生长因子(TGFβ1-CTGF)途径在体内发挥抑制大鼠系膜增生和系膜基质扩张的作用.方法 制备大鼠抗Thy-1系膜增生性肾炎模型,同时给予FTY720干预,观察3组大鼠24 h尿蛋白定量、肾小球系膜增生程度,并分离肾小球进行培养,检测培养上清细胞外基质纤维连接蛋白、层黏蛋白和Ⅳ型胶原蛋白分泌水平变化,即时定量聚合酶链反应(RT-PCR)检测肾小球TGFβ1和CTGF基因表达,Western blot检测肾小球TGFβ1和CTGF蛋白表达.结果 在注射抗体后第1、3、7天,模型组尿蛋白[(27.9±7.3)、(63.5±18.8)、(52.4±15.4) mg/d]较对照组[(8.4±2.4)、(8.4±2.1)、(10.4±3.2)mg/d]明显增多,差异均有统计学意义(均P<0.01).FTY720干预组第3、7天尿蛋白[(31.4±7.0)、(25.5±7.7)mg/d]与模型组相比明显减少,但仍显著高于对照组,各组间差异有统计学意义(均P<0.01).注射后第3、7天,FTY720可以显著减少TGFβ1和CTGF基因以及蛋白表达,减低肾小球细胞外基质蛋白分泌,减轻肾小球系膜增生程度.结论 FTY720可以通过抑制TGFβl-CTGF途径显著减轻模型大鼠系膜增生和系膜基质扩张,干预抗Thy-1系膜增生性肾炎的发生.%Objective To investigate whether FTY720 inhibits rat mesangial proliferation and extracellular matrix expansion through suppression of transforming growth factor β1-connective tissue growth factor (TGFβ1-CTGF) pathway,and to explore experimental evidence for its effect on mesangial proliferative glomerulonephritis.Methods A rat model of anti-Thy-1 mesangial proliferative glomerulonephritis was established and FTY720 intervention was performed. Periphery blood lymphocyte count,urine protein excretion,glomerular mesangial proliferation,protein and gene expression of TGFβ1 and CTGF and extracellular matrix protein including fibronectin,laminin and collagen IV in isolated glomeruli were

  16. Incidence and Remission of Parasomnias among Adolescent Children in the Tucson Children's Assessment of Sleep Apnea (TuCASA) Study.

    Science.gov (United States)

    Furet, Oscar; Goodwin, James L; Quan, Stuart F

    2011-01-01

    BACKGROUND: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group. METHODS: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1) and subsequently restudied them approximately 5 years later (Time 2). At both time points parents were asked to complete a comprehensive sleep habits questionnaire designed to assess the severity of sleep-related symptoms that included questions about enuresis (EN), sleep terrors (TR), sleep walking (SW) and sleep talking (ST). RESULTS: There were 350 children participating at Time 1 who were studied as adolescents at time 2. The mean interval between measurements was (4.6 years). The incidence of EN, TR, ST, and SW in these 10-18 year old children was 0.3%, 0.6%, 6.0% and 1.1% respectively. Remission rates were 70.8%, 100%, 64.8% and 50.0% respectively. CONCLUSIONS: The incidence rates of EN, TR, and SW were relatively low moving from childhood to adolescence while remission rates were high across all parasomnias.

  17. Control de criaderos de Aedes aegypti con el programa Recicla por tu bienestar en Mérida, México

    Directory of Open Access Journals (Sweden)

    Mario Barrera Pérez

    2015-05-01

    Full Text Available Objetivos. Determinar la importancia de los criaderos de Ae. aegypti en Mérida; evaluar el impacto del programa Recicla or tu bienestar (RxB sobre la presencia/abundancia de éstos y la percepción de los habitantes. Material y métodos. Se calculó la importancia de los criaderos por su productividad pupal. Se realizaron muestreos pre y post RxB en colonias para cuantificar el total de recipientes/criaderos. Se aplicó una encuesta a participantes sobre la percepción sobre RxB en colonias seleccionadas. Resultados. Los botes, cubetas y diversos objetos chicos fueron los criaderos más importantes. RxB tuvo un impacto significativo en la reducción del número de recipientes (IRR=0.74, en los recipientes positivos (IRR=0.33 y en la positividad de las viviendas para Ae. aegypti (OR=0.41. Todos los entrevistados opinaron que RxB es necesario la gran mayoría piensa que es útil. Conclusiones. RxB debe ser considerada una buena práctica para el control del vector del dengue.

  18. Increasing HIV-related knowledge, communication, and testing intentions among Latinos: Protege tu Familia: Hazte la Prueba.

    Science.gov (United States)

    Rios-Ellis, Britt; Espinoza, Lilia; Bird, Mara; Garcia, Melawhy; D'Anna, Laura Hoyt; Bellamy, Laura; Scolari, Rosana

    2010-08-01

    Latinos are less likely to be aware of their HIV seropositivity than African Americans and Whites. 'Protege tu Familia: Hazte la Prueba' is a culturally and linguistically-sensitive HIV/AIDS prevention and testing program targeting Latino families. Using community-based participatory research techniques, Spanish-speaking bicultural community health workers helped develop and then used an educational flip chart and materials to conduct outreach and HIV prevention education in diverse settings. The intervention was created to increase HIV/AIDS-related knowledge, to improve communication regarding sexual risk, and to augment intentions to use condoms and test for HIV. A secondary purpose was to decrease HIV-related stigma by improving knowledge about transmission and reducing homophobia. Participants demonstrated significant increases in HIV knowledge, intention to practice safer sex and communicate sexual risk to partner(s), and intention to test for HIV. Improvements were also found in self-reported comfort levels when interacting with and caring for the HIV positive, thus decreasing HIV/AIDS-related stigma.

  19. The dynamic atmospheres of Mira stars: comparing the CODEX models to PTI time series of TU And

    CERN Document Server

    Hillen, M; Degroote, P; Acke, B; van Winckel, H

    2012-01-01

    Our comprehension of stellar evolution on the AGB still faces many difficulties. To improve on this, a quantified understanding of large-amplitude pulsator atmospheres and interpretation in terms of their fundamental stellar parameters are essential. We wish to evaluate the effectiveness of the recently released CODEX dynamical model atmospheres in representing M-type Mira variables through a confrontation with the time-resolved spectro-photometric and interferometric PTI data set of TU And. We calibrated the interferometric K-band time series to high precision. This results in 50 nights of observations, covering 8 subsequent pulsation cycles. At each phase, the flux at 2.2$\\mu$m is obtained, along with the spectral shape and visibility points in 5 channels across the K-band. We compared the data set to the relevant dynamical, self-excited CODEX models. Both spectrum and visibilities are consistently reproduced at visual minimum phases. Near maximum, our observations show that the current models predict a pho...

  20. The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) cleaves amyloid-β.

    Science.gov (United States)

    Han, Hye-Eun; Sellamuthu, Saravanan; Shin, Bae Hyun; Lee, Yong Jae; Song, Sungmin; Seo, Ji-Seon; Baek, In-Sun; Bae, Jeomil; Kim, Hannah; Yoo, Yung Joon; Jung, Yong-Keun; Song, Woo Keun; Han, Pyung-Lim; Park, Woo Jin

    2010-12-20

    The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12)-His-His-Gln(15), near the presumptive α-secretase cleavage site of the amyloid-β (Aβ) peptide led us to hypothesize that NIa could possess activity against Aβ. Western blotting results showed that oligomeric as well as monomeric forms of Aβ can be degraded by NIa in vitro. The specific cleavage of Aβ was further confirmed by mass spectrometry analysis. NIa was shown to exist predominantly in the cytoplasm as observed by immunofluorescence microscopy. The overexpression of NIa in B103 neuroblastoma cells resulted in a significant reduction in cell death caused by both intracellularly generated and exogenously added Aβ. Moreover, lentiviral-mediated expression of NIa in APP(sw)/PS1 transgenic mice significantly reduced the levels of Aβ and plaques in the brain. These results indicate that the degradation of Aβ in the cytoplasm could be a novel strategy to control the levels of Aβ, plaque formation, and the associated cell death.

  1. The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV cleaves amyloid-β.

    Directory of Open Access Journals (Sweden)

    Hye-Eun Han

    Full Text Available BACKGROUND: The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12-His-His-Gln(15, near the presumptive α-secretase cleavage site of the amyloid-β (Aβ peptide led us to hypothesize that NIa could possess activity against Aβ. METHODOLOGY/PRINCIPAL FINDINGS: Western blotting results showed that oligomeric as well as monomeric forms of Aβ can be degraded by NIa in vitro. The specific cleavage of Aβ was further confirmed by mass spectrometry analysis. NIa was shown to exist predominantly in the cytoplasm as observed by immunofluorescence microscopy. The overexpression of NIa in B103 neuroblastoma cells resulted in a significant reduction in cell death caused by both intracellularly generated and exogenously added Aβ. Moreover, lentiviral-mediated expression of NIa in APP(sw/PS1 transgenic mice significantly reduced the levels of Aβ and plaques in the brain. CONCLUSIONS/SIGNIFICANCE: These results indicate that the degradation of Aβ in the cytoplasm could be a novel strategy to control the levels of Aβ, plaque formation, and the associated cell death.

  2. First intercomparison among laboratories involved in COST Action-TU1301 "NORM4Building": Determination of natural radionuclides in ceramics.

    Science.gov (United States)

    Xhixha, Gerti; Trinidad, José Antonio; Gascó, Catalina; Mantovani, Fabio

    2017-03-01

    This work describes the outcomes of the COST Action-TU1301 "NORM4Building" intercomparison on the determination of natural radioactivity in ceramics. Twenty-two laboratories involved in the intercomparison are evaluated for their performance using robust statistics. The reference values of (226)Ra ((214)Bi and (214)Pb) are determined to be 122 ± 11 Bq kg(-1) and 124 ± 14 Bq kg(-1), respectively and in secular equilibrium in the uranium chain while the reference values of (232)Th ((228)Ac) is determined to be 61 ± 6 Bq kg(-1) and that of (40)K was determined to be 955 ± 40 Bq kg(-1). Although the aim of the exercise was to determine the activity concentration of (226)Ra, (232)Th and (40)K and evaluation of the "Activity Concentration Index", laboratories were asked to report complete characterization of natural radionuclides. The results of this exercise pointed out a good performance among laboratories since the percentage of the acceptable results were above 90% for the radionuclides of interest. Based on these results, considering the systematic rejection of the results reported from a few laboratories we emphasize the need for quality control procedures. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Applications of GPR in archaeological prospecting and cultural heritage diagnostics: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Schettini, Giuseppe; Soldovieri, Francesco

    2013-04-01

    Ground Penetrating Radar (GPR) is a safe, non-destructive and non-invasive imaging technique that can be effectively used for advanced inspection of composite structures and for diagnostics affecting the whole life-cycle of civil engineering works. GPR can also be successfully employed in archaeological prospecting and cultural heritage diagnostics. In many Countries, where the archeological patrimony is an outstanding value (as Egypt, Israel, Greece, Central and South America), GPR is usually employed both as a diagnostic tool for the preventive detection of archeological structures and as the most advanced instrument able to prospect geometry and shape of underground valuable sites. However many uncertainties persist, because of several difficulties and ambiguities due to the complexity of the image processing in heterogeneous environment. It is possible to identify three main areas, in GPR field, that have to be addressed in order to promote the use of this technology in archaeological prospecting and cultural heritage diagnostics. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions, archeological sites are often located in impervious and critical environments; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. It is also important to further investigate and promote a combined use of GPR with other non-invasive advanced techniques, typically used in the archeological investigation. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by a research team of "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE

  4. [Carbohydrate antigens CA 19-9, CA 242, CA 50 in liver diseases].

    Science.gov (United States)

    Nowak, J; Jakubowska, D; Wiczkowski, A; Sprzaczkowska, K; Stechły, T; Zmudziński, W; Grzesik, P; Walas, R; Jarzab, B

    1998-01-01

    Serum concentrations of CA 19-9, CA 242, and CA 50 were determined in patients with hepatitis and liver cirrhosis without cholestasis. The study included 63 patients with chronic persistent hepatitis (group A), chronic active hepatitis (group B), and liver cirrhosis (group C). The control group (K) consisted of 82 patients with: peptic ulcer, colorectal polypi or diverticulosis of the colon. CA 19-9 level normal in the majority of patients with liver diseases, however, it was found to be increased in 4 (23%) of patients with liver cirrhosis. There was no statistically significant difference in the frequency of increased level of CA 19-9 between liver diseases and the control group. The rate of elevated serum level of CA 242 in patients with liver diseases and in control group was similar respectively 12%; 8.5%). The elevated CA 50 levels were most frequently found in patients with liver pathology (50% in liver cirrhosis and chronic active hepatitis; 36% in chronic persistent hepatitis). The elevation of CA 50 serum level occurs very often in liver diseases, even when they are going without cholestasis. Thus, the antigen is not useful for differentiating between benign and cancer diseases of gastrointestinal tract. Antigen CA 50 is to be taken into account only after exclusion of the pathology of liver, especially cirrhosis. Other investigated antigens: CA 19-9 and CA 242 are influenced by liver diseases to a minor and neglectable extent. Antigen CA 19-9 is the marker of choice in gastrointestinal cancers.

  5. 清热化痰法治疗急性耳鸣的THI量表初步评价%Clearing Heat and Resolving Phlegm Method in the Treatment of Acute Tinnitus THI Scale Preliminary Evaluation

    Institute of Scientific and Technical Information of China (English)

    杨梅红; 冯丽伟

    2015-01-01

    Objective: Toevaluate the clinical effect of clearing heat and resolving phlegm method in the treatment of acute tinnitus and explore its theoretical basis.Methods:59 cases of acute tinnitus patients,application of ten flavor Longdanhua granules,treatment for 2 weeks,before and after the treatment by tinnitus handicap inventory (THI) clinical observation.Results:After treatment,the total effective rate was 88.14%,and the Ten flavor Longdanhua granules in improving mood index than the other two groups of indexes have more advantage (P<0.05).Conclusion:The curative effect of Ten flavor Longdanhua granules in the treatment of acute tinnitus is obvious,clearing heat and resolving phlegm method has an important significance for the treatment of acute tinnitus.%目的:评价清热化痰法治疗急性耳鸣的临床疗效并探讨其理论依据.方法:59例急性耳鸣患者,应用十味龙胆花颗粒口服,疗程2周,治疗前后通过耳鸣残疾评估量表(THI)观察临床疗效.结果:治疗后总有效率达88.14%,且十味龙胆花颗粒在改善情绪类指标上较其他两组指标更有优势(P<0.05).结论:十味龙胆花颗粒治疗急性耳鸣的疗效明显,清热化痰法对于治疗急性耳鸣具有重要的意义.

  6. Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L. ssp. pekinensis%一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发

    Institute of Scientific and Technical Information of China (English)

    田希辉; 于拴仓; 苏同兵; 张凤兰; 余阳俊; 张德双; 赵岫云; 汪维红

    2014-01-01

    为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期TuMV-C4抗性相关的主效QTL位点BrTuA09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个InDel在双亲间具有多态性,且条带单一、扩增稳定;连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTuA09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜TuMV抗病分子育种奠定了良好的基础。%Our research constructed a molecular genetic map with a line 91-112 ( highly resistant to TuMV-C4),a highly susceptible line T12-19 and the DH population derived from microspore culture of F1(91-112 × T12-19). A total of 45 markers were mapped to the A09 chromosome,and a new QTL-BrTuA09 controlling TuMV-C4 re-sistance was identified. On this basis,27 pairs of primers were designed by screening the InDel loci on A09 chromo-some of Chinese cabbage based on genome re-sequencing. The results showed that distinct PCR amplification prod-ucts were obtained from the InDel markers. Among these markers,11 InDel were polymorphic on the tested parent materials,and mapped to the A09 chromosome. By linkage analysis,these markers were tightly linked to the QTL-BrtuA09 . The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hy-brid breeding programs and improve the resistance to TuMV-C4 .

  7. TU-F-17A-03: An Analytical Respiratory Perturbation Model for Lung Motion Prediction

    Energy Technology Data Exchange (ETDEWEB)

    Li, G; Yuan, A [Memorial Sloan Kettering Cancer Center, New York, NY (United States); Wei, J [City College of New York, New York, NY (United States)

    2014-06-15

    training, and therefore is potentially more resilient to breathing irregularities. On-going investigation introduces airflow into the RMP model for improvement. This research is in part supported by NIH (U54CA137788/132378). AY would like to thank MSKCC summer medical student research program supported by National Cancer Institute and hosted by Department of Medical Physics at MSKCC.

  8. Biological Characteristics of CD133+CD44+ Cancer Stem Cells Sorting from Laryngeal Carcinoma Cell Line TU177%喉癌TU177细胞系中CD133+CD44+肿瘤干细胞分选及特性分析

    Institute of Scientific and Technical Information of China (English)

    杨俊岭; 高伟; 王珏; 付荣; 陈波; 李伟艳; 温树信; 王斌全

    2016-01-01

    Objective :Magnetic activated cell sorting was used to separate CD133+CD44+ cancer cells from laryngeal car-cinoma TU177 cell line. Analysis the biological characteristics of these subpopulations .Methods :TU177 cells were subjected to magnetic activated cell sorting to obtain CD133+CD44+、CD133+CD44-、CD133-CD44+、CD133-CD44-cells. Evaluate the efficiency of magnetic separation by flow cytometry . Test cell proliferation,migration,invasion,adhesion,colony forming ability of the cells.Results: CD133+CD44+ cells show higher proliferation,migration,invasion,adhesion,clone ability than other group(P<0.0001).Conclusions:TU177 cells can be serparated by Magnetic activated cell sorting effectively. CD133 is more powerful than CD44.Our study may provide evidence for target treatment of laryngeal cancer.%目的:免疫磁珠分选喉癌TU177细胞系中的CD133+CD44+细胞,探讨CD133+CD44+细胞作为肿瘤干细胞的生物学特性。方法:培养喉癌TU177细胞,采用免疫磁珠分选技术分选CD133+CD44+、CD133+CD44-、CD133-CD44+、CD133-CD44-细胞,流式检测分选效率,检测各组细胞的增殖、侵袭、迁移、粘附、克隆形成能力。结果:CD133+CD44+细胞的增殖、迁移、侵袭、粘附、克隆能力均明显高于其他组,差异有统计学意义(P<0.0001)。结论:免疫磁珠技术能有效进行TU177细胞系的分选,CD133+CD44+细胞亚群具有强增殖、侵袭、迁移、粘附、克隆形成能力,具有肿瘤干细胞特征,CD133作为干细胞标志物,其干细胞特性强于CD44,可为喉癌的进一步靶向治疗提供依据。

  9. Exchanging knowledge and working together in COST Action TU1208: Short-Term Scientific Missions on Ground Penetrating Radar

    Science.gov (United States)

    Santos Assuncao, Sonia; De Smedt, Philippe; Giannakis, Iraklis; Matera, Loredana; Pinel, Nicolas; Dimitriadis, Klisthenis; Giannopoulos, Antonios; Sala, Jacopo; Lambot, Sébastien; Trinks, Immo; Marciniak, Marian; Pajewski, Lara

    2015-04-01

    This work aims at presenting the scientific results stemming from six Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (Action Chair: Lara Pajewski, STSM Manager: Marian Marciniak). STSMs are important means to develop linkages and scientific collaborations between participating institutions involved in a COST Action. Scientists have the possibility to go to an institution abroad, in order to undertake joint research and share techniques/equipment/infrastructures that may not be available in their own institution. STSMs are particularly intended for Early Stage Researchers (ESRs), i.e., young scientists who obtained their PhD since no more than 8 years when they started to be involved in the Action. Duration of a standard STSM can be from 5 to 90 days and the research activities carried out during this short stay shall specifically contribute to the achievement of the scientific objectives of the supporting COST Action. The first STSM was carried out by Lara Pajewski, visiting Antonis Giannopoulos at The University of Edinburgh (United Kingdom). The research activities focused on the electromagnetic modelling of Ground Penetrating Radar (GPR) responses to complex targets. A set of test scenarios was defined, to be used by research groups participating to Working Group 3 of COST Action TU1208, to test and compare different electromagnetic forward- and inverse-scattering methods; these scenarios were modelled by using the well-known finite-difference time-domain simulator GprMax. New Matlab procedures for the processing and visualization of GprMax output data were developed. During the second STSM, Iraklis Giannakis visited Lara Pajewski at Roma Tre University (Italy). The study was concerned with the numerical modelling of horn antennas for GPR. An air-coupled horn antenna was implemented in GprMax and tested in a realistically

  10. “MONTEVIDEO, TU CASA”: ELEMENTOS PARA UNA LECTURA DEL CUERPO EN EL ESPACIO PÚBLICO MODERNO.

    Directory of Open Access Journals (Sweden)

    Cecilia Seré Quintero

    2015-11-01

    Full Text Available El eslogan “Montevideo, tu casa” utilizado por la Intendencia Municipal de Montevideo (Uruguay entre 1991 y 2006, funciona como punto de partida para el análisis de las formas modernas que toma el espacio público urbano. La ciudad, escenario de la modernidad, da cuenta de la indistinción que se establecerá entre lo público y lo privado a partir de la emergencia de lo social, la consolidación del Estado-nación y la configuración de la economía política como parámetro de lectura de la realidad. Con ello, la política se verá reducida a la gestión y administración de las necesidades de la población, y la vida en términos biológicos tendrá allí un lugar central, factor clave para la organización de un espacio público que se presenta como una extensión del ámbito doméstico. El proceso de modernización de la ciudad de Montevideo es tomado en este artículo para dar cuenta de los efectos de una política contemporánea que se rige por la primacía de lo económico y donde parecen reducirse las distinciones entre el espacio público y el doméstico a cuestiones de tamaño o escala, teniendo como correlato una biologización de la vida pública y por tanto de la política.

  11. Ground-penetrating radar investigation of St. Leonard's Crypt under the Wawel Cathedral (Cracow, Poland) - COST Action TU1208

    Science.gov (United States)

    Benedetto, Andrea; Pajewski, Lara; Dimitriadis, Klisthenis; Avlonitou, Pepi; Konstantakis, Yannis; Musiela, Małgorzata; Mitka, Bartosz; Lambot, Sébastien; Żakowska, Lidia

    2016-04-01

    The Wawel ensemble, including the Royal Castle, the Wawel Cathedral and other monuments, is perched on top of the Wawel hill immediately south of the Cracow Old Town, and is by far the most important collection of buildings in Poland. St. Leonard's Crypt is located under the Wawel Cathedral of St Stanislaus BM and St Wenceslaus M. It was built in the years 1090-1117 and was the western crypt of the pre-existing Romanesque Wawel Cathedral, so-called Hermanowska. Pope John Paul II said his first Mass on the altar of St. Leonard's Crypt on November 2, 1946, one day after his priestly ordination. The interior of the crypt is divided by eight columns into three naves with vaulted ceiling and ended with one apse. The tomb of Bishop Maurus, who died in 1118, is in the middle of the crypt under the floor; an inscription "+ MAVRVS EPC MCXVIII +" indicates the burial place and was made in 1938 after the completion of archaeological works which resulted in the discovery of this tomb. Moreover, the crypt hosts the tombs of six Polish kings and heroes: Michał Korybut Wiśniowiecki (King of the Polish-Lithuanian Commonwealth), Jan III Sobieski (King of the Polish-Lithuanian Commonwealth and Commander at the Battle of Vienna), Maria Kazimiera (Queen of the Polish-Lithuanian Commonwealth and consort to Jan III Sobieski), Józef Poniatowski (Prince of Poland and Marshal of France), Tadeusz Kościuszko (Polish general, revolutionary and a Brigadier General in the American Revolutionary War) and Władysław Sikorski (Prime Minister of the Polish Government in Exile and Commander-in-Chief of the Polish Armed Forces). The adjacent six crypts and corridors host the tombs of the other Polish kings, from Sigismund the Old to Augustus II the Strong, their families and several Polish heroes. In May 2015, the COST (European COoperation in Science and Technology) Action TU1208 "Civil engineering applications of Ground Penetrating Radar" organised and offered a Training School (TS) on the

  12. Tu-yuan-ce-fu and Compilation of Encyclopedia in the Tang Dynasty%《兔园策府》与唐代类书的编纂

    Institute of Scientific and Technical Information of China (English)

    王璐

    2015-01-01

    类书起源很早,是中国传统文化的产物。唐代修撰类书,始于建国之初,大抵是出于帝王之愿,试图把所有的知识进行分类便于提纲挈领地学习,因而唐代还有不少文人学士所编的类书就因循这样的法则。敦煌本《兔园策府》也是其中之一,它的白文本在唐宋间十分流行,《兔园策府》成书时间在《初学记》前,因而偶句之体例不是自徐坚始,很有可能始自《兔园策府》。%The encyclopedia originated early, is the product of Chinese traditional culture. The encyclopedias com-pilation started at the foundation of Tang Dynasty, which is probably out of the emperor willing to try to put all the knowledge classification facilitates learning focused on the vital. Then the encyclopedias compiled by scholars was procrastinating this rule as well in Tang Dynasty. Tu-yuan-ce-fu ( Encyclopedia Rabbit Garden) of Dunhuang edi-tion is one of them;its full form was very popular among the Tang and Song Dynasties. Tu-yuan-ce-fu was comple-ted before Chu-xue-ji ( The Primary Anthology) . Alternate style was not created by Xu Jian but probably from Tu-yuan-ce-fu.

  13. Tu-yuan-ce-fu and Compilation of Encyclopedia in the Tang Dynasty%《兔园策府》与唐代类书的编纂

    Institute of Scientific and Technical Information of China (English)

    王璐

    2014-01-01

    类书起源很早,是中国传统文化的产物。唐代修撰类书,始于建国之初,大抵是出于帝王之愿,试图把所有的知识进行分类便于提纲挈领地学习,因而唐代还有不少文人学士所编的类书就因循这样的法则。敦煌本《兔园策府》也是其中之一,它的白文本在唐宋间十分流行,《兔园策府》成书时间在《初学记》前,因而偶句之体例不是自徐坚始,很有可能始自《兔园策府》。%The encyclopedia originated early, is the product of Chinese traditional culture. The encyclopedias com-pilation started at the foundation of Tang Dynasty, which is probably out of the emperor willing to try to put all the knowledge classification facilitates learning focused on the vital. Then the encyclopedias compiled by scholars was procrastinating this rule as well in Tang Dynasty. Tu-yuan-ce-fu ( Encyclopedia Rabbit Garden) of Dunhuang edi-tion is one of them;its full form was very popular among the Tang and Song Dynasties. Tu-yuan-ce-fu was comple-ted before Chu-xue-ji ( The Primary Anthology) . Alternate style was not created by Xu Jian but probably from Tu-yuan-ce-fu.

  14. Transcriptional Profiling of Newly Generated Dentate Granule Cells Using TU Tagging Reveals Pattern Shifts in Gene Expression during Circuit Integration1,2

    Science.gov (United States)

    Chatzi, Christina; Shen, Rongkun; Goodman, Richard H.

    2016-01-01

    Abstract Despite representing only a small fraction of hippocampal granule cells, adult-generated newborn granule cells have been implicated in learning and memory (Aimone et al., 2011). Newborn granule cells undergo functional maturation and circuit integration over a period of weeks. However, it is difficult to assess the accompanying gene expression profiles in vivo with high spatial and temporal resolution using traditional methods. Here we used a novel method [“thiouracil (TU) tagging”] to map the profiles of nascent mRNAs in mouse immature newborn granule cells compared with mature granule cells. We targeted a nonmammalian uracil salvage enzyme, uracil phosphoribosyltransferase, to newborn neurons and mature granule cells using retroviral and lentiviral constructs, respectively. Subsequent injection of 4-TU tagged nascent RNAs for analysis by RNA sequencing. Several hundred genes were significantly enhanced in the retroviral dataset compared with the lentiviral dataset. We compared a selection of the enriched genes with steady-state levels of mRNAs using quantitative PCR. Ontology analysis revealed distinct patterns of nascent mRNA expression, with newly generated immature neurons showing enhanced expression for genes involved in synaptic function, and neural differentiation and development, as well as genes not previously associated with granule cell maturation. Surprisingly, the nascent mRNAs enriched in mature cells were related to energy homeostasis and metabolism, presumably indicative of the increased energy demands of synaptic transmission and their complex dendritic architecture. The high spatial and temporal resolution of our modified TU-tagging method provides a foundation for comparison with steady-state RNA analyses by traditional transcriptomic approaches in defining the functional roles of newborn neurons. PMID:27011954

  15. TU-E-201-02: Eye Lens Dosimetry From CT Perfusion Studies

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, D. [Toshiba America Medical Systems (United States)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  16. TU-E-201-00: Eye Lens Dosimetry for Patients and Staff

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  17. 菌株Tu 49 代谢产物的分离及抗菌素活性的初步研究%The Isolation of the Strain Tu49 and the Study on the Activity of Its Antibiosis

    Institute of Scientific and Technical Information of China (English)

    迪力夏提·托呼提; 阿不都克里木·热依木

    2001-01-01

    从吐鲁番葡萄沟、苏公塔、五道林、交角沟等郊区采集的52种土样中筛选到一株能产有抗菌素活性的好气菌Tu49,革兰氏阳性,类群为黄色类群。所产生的抗菌素,对不同的微生物有明显的抑制作用。最适生长温度为30℃~32℃,最适pH为6.8~7.5,气生菌丝和孢子丝为无色,基丝体为黄色,产黄色可溶性色素。%We isolated aerial strain Tu 49 from 52 samples of soil in 5 outskirts of Turpan city, its colony was golden and Gram positives(G+).The antibiosis from strain Tu49 inhibited different microorganisms, The optimum growth temperature was 30~32℃ and pH was 6.8~7.5;aerial hypha and spore hypha was colourless, my cellium was yellow. Which can produce soluble figment.

  18. [Classes of crude drugs and its distribution of producing area in the attached illustrations in Ben cao tu jing (Illustrated Classic of Materia Medica)].

    Science.gov (United States)

    Xu, T; Peng, H S

    2016-03-01

    Ben cao tu jing (Illustrated Classic of Materia Medica) is the earliest extant atlas book of materia medica in China, with 933 attached drawings. Among them, the largest portion, amounting to 670, are herbaceous plants, mostly commonly used, with definite marks of the origin producing areas, distributed across 149 administrative divisions(prefectures and counties) of the Song Dynasty, most of them in Northern area which were distributed denser than those in Southern area. The densest ones were located in Southern Shanxi, Eastern Sichuan and Eastern Anhui. In the attached drawings, the frequency of highest occurrence appeared in this Classic are three prefectures, Chuzhou, Shizhou and Guangzhou.

  19. The Comparative Study between“Ni/Nin”in Chinese and“Tu/Vous”in French Based on“Power and Solidarity”

    Institute of Scientific and Technical Information of China (English)

    杨悦

    2012-01-01

      The selection of the second person pronouns embodies the power and solidarity relations in social communication. The paper studies“Ni/Nin”in Chinese and“Tu/Vous”in French from sociolinguistic, cultural and pragmatic perspectives by basing on“Power and Solidarity”. Through the comparative study of this two pairs, not only can we observe the historical process of personal pronouns, and analyze the similarities and dissimilarities of the two languages which hide behind Chinese and French cultural backgrounds, but also may further understand their pragmatic principles in various context.

  20. Identification of probiotics strain Tu-569 from rabbit cecum and property analysis of its extracellular antibacterial product%兔源益生菌Tu-569菌株鉴定与其胞外产抑菌物性质分析

    Institute of Scientific and Technical Information of China (English)

    柴玉龙; 尚伟; 姜军坡; 王世英; 朱宝成

    2012-01-01

    目的 对兔源益生菌Tu-569菌株进行菌落形态和菌体形态观察、生理生化特性测定及16S rDNA序列系统发育分析以确定其种属.方法 以大肠埃希菌为指示菌,通过琼脂打孔扩散法分析Tu-569菌株胞外产抑菌物性质,初步评价该抗菌物活性.结果 Tu-569菌株为Bacillus velezensis; Tu-569菌株胞外产抑菌物为分子量3.5~8.0kD的多肽,可被饱和度60%的硫酸铵盐析;该抗菌肽热稳定性较好,但可被蛋白酶降解;采用正丁醇萃取该抗菌肽不会降低其活性;其活性可被Mn2+离子增强,但在强酸(pH 3)强碱(pH11)环境中会大幅度降低;当存在表面活性剂和β-巯基乙醇时,抗菌肽抑菌活性完全丧失.每1mg粗蛋白中含有的抗菌肽抑制大肠埃希菌的能力相当于2μg硫酸庆大霉素、10μg硫酸链霉素或50μg土霉素.结论 该抗菌肽性质稳定,具有较高的抗菌活性,在畜牧业、养殖业、食品等领域中有一定的应用价值.%Objective Works were done to identify probiotic strain Tu-569 and to analyze properties of its extracellular antibacterial product. Methods Identification was carried out by observations for colony morphology and cell shape, determinations of physiological, biochemical characteristics and sequence analysis. Properties analysis for extracellular antibacterial product were done with various treatments, such as salting out with ammonium sulfate, extraction with different organic solvent, heating, different pH environment, proteases, ions, protein protective and denaturant agents. The activity of antibacterial product was evaluated by agar diffusion assay with Escherichia coli as indicator bacteria. Results Strain Tu-569 was identified as Bacillus velezensis. The extracellular antibacterial product was polypeptide with molecular weight between 3.5kD to 8.0kD. The majority antibacterial polypeptide was salted out by 60% ammonium sulfate. The antibacterial polypeptide showed a good stability

  1. PID参数继电反馈整定法的改进%Improvement of relay feedback tuing method of PID parameters

    Institute of Scientific and Technical Information of China (English)

    高峰

    2012-01-01

    In this paper,the relay feedback tuing method of PID parameters is improved.An integrator is set between the relay and controlled object. High frequency signal is weaken, the proportion of the fundamental wave in the harmonic wave is increased and the accuracy of describing function is improved.On this occasion,new tuing equations is presented. Practical emulation proves that the tuning method after improvement can get better performance than Astrom method and PM method.%提出了一种改进的继电整定法,在继电环节和被控对象之间设置一个积分器。促进了高频信号的衰减。使谐波中的基波成分所占比例得到提高,改善了描述函数的精度,并在此情况下推导出了新的整定公式。通过仿真证明。利用改进后的方法进行PID参数整定比传统的Astrom法和PM法性能更加优越。

  2. Raúl Gonzalez Tuñón desencuadernado. Políticas de la literatura, entre el libro y las publicaciones periódicas

    Directory of Open Access Journals (Sweden)

    Geraldine Rogers

    2015-11-01

    Full Text Available RESUMEN: el artículo revisa algunos supuestos sobre la producción literaria de Raúl González Tuñón en las décadas de 1920 y 1930 a partir de dos desplazamientos. En primer lugar, explora las políticas de la literatura que permitirían pensar, de manera menos esquemática que con la habitual categoría de “literatura política” con que se suelen abordar sus textos de la década del treinta, las apuestas y limitaciones de una escritura que desde su inicio se propuso como transformadora. En segundo lugar, el artículo pone el foco en la primera circulación de los textos, muchas veces en diarios y revistas, contextos formativos de una literatura que en gran medida surgió en sus páginas. Desencuadernar a Tuñón implica atender a la materialidad de sus escritos y explorar más allá de la tradicional figura del autor de libros, para indagar políticas de la escritura que articularon campos y prácticas, y experimentaron no sólo con nuevas formas estéticas sino también con diversos modos de circulación pública de las letras.

  3. 土族民间信仰的民族心理学解析%Tu Folk Beliefs of the National Psychology Analytical

    Institute of Scientific and Technical Information of China (English)

    王默

    2011-01-01

    Tu folk circulates with many unique folk beliefs,the folk beliefs are minority is an important part of traditional culture.Including fertility belief,reproductive worship,home most widely and typical worship god.This paper tries to ethnic psychology,reading from the perspective of representative of folk beliefs of tu folk beliefs,to explore the psychological factors produced.%土族民间存在着很多独具特色的民间信仰,这些民间信仰是土族传统文化的重要组成部分。其中丰产信仰、生殖崇拜、家神崇拜最为广泛和典型。本文试从民族心理学的角度入手,解读其中具有代表性的民间信仰,以探讨土族民间信仰产生的心理因素。

  4. GTPase domains of ras p21 oncogene protein and elongation factor Tu: analysis of three-dimensional structures, sequence families, and functional sites.

    Science.gov (United States)

    Valencia, A; Kjeldgaard, M; Pai, E F; Sander, C

    1991-06-15

    GTPase domains are functional and structural units employed as molecular switches in a variety of important cellular functions, such as growth control, protein biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures of only mammalian ras p21 and bacterial elongation factor Tu have been determined. After optimal superposition of these remarkably similar structures, careful multiple sequence alignment, and calculation of residue-residue interactions, we analyzed the two subfamilies in terms of structural conservation, sequence conservation, and residue contact strength. There are three main results. (i) A structure-based alignment of p21 and elongation factor Tu. (ii) The definition of a common conserved structural core that may be useful as the basis of model building by homology of the three-dimensional structure of any GTPase domain. (iii) Identification of sequence regions, other than the effector loop and the nucleotide binding site, that may be involved in the functional cycle: they are loop L4, known to change conformation after GTP hydrolysis; helix alpha 2, especially Arg-73 and Met-67 in ras p21; loops L8 and L10, including ras p21 Arg-123, Lys-147, and Leu-120; and residues located spatially near the N and C termini. These regions are candidate sites for interaction either with the GTP/GDP exchange factor, with a GTPase-affected function, or with a molecule delivered to a destination site with the aid of the GTPase domain.

  5. Electromagnetic modelling, inversion and data-processing techniques for GPR: ongoing activities in Working Group 3 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonis; van der Kruk, Jan

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 3 (WG3) 'EM methods for near-field scattering problems by buried structures; data processing techniques' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. WG3 is structured in four Projects. Project 3.1 deals with 'Electromagnetic modelling for GPR applications.' Project 3.2 is concerned with 'Inversion and imaging techniques for GPR applications.' The topic of Project 3.3 is the 'Development of intrinsic models for describing near-field antenna effects, including antenna-medium coupling, for improved radar data processing using full-wave inversion.' Project 3.4 focuses on 'Advanced GPR data-processing algorithms.' Electromagnetic modeling tools that are being developed and improved include the Finite-Difference Time-Domain (FDTD) technique and the spectral domain Cylindrical-Wave Approach (CWA). One of the well-known freeware and versatile FDTD simulators is GprMax that enables an improved realistic representation of the soil/material hosting the sought structures and of the GPR antennas. Here, input/output tools are being developed to ease the definition of scenarios and the visualisation of numerical results. The CWA expresses the field scattered by subsurface two-dimensional targets with arbitrary cross-section as a sum of cylindrical waves. In this way, the interaction is taken into account of multiple scattered fields within the medium hosting the sought targets. Recently, the method has been extended to deal with through-the-wall scenarios. One of the

  6. Solid Phase Extraction of Bryophyte Lipids / Briofītu lipīdu cietfāzes ekstrakcijas frakcionēšana

    Directory of Open Access Journals (Sweden)

    Klavina Laura

    2015-11-01

    Full Text Available Pētījuma ietvaros izstrādāta vienkārša, ātra un viegli atkārtojama analītiska metode briofītu lipīdu frakcionēšanai. Lipīdu frakcijas frakcionēšanai izmantoja cietfāzes ekstrakciju ar silikagēlu un ar NH2 grupām modificētu silikagēlu, iegūtās vielu grupas kvantificēja kā to trimetilsilil atvasinājumus, izmantojot gāzes hromatogrāfiju-masas spektrometriju. Frakcionēšanu veica, izmantojot eluentu polaritātes gradienta principu (eluenti: heksāns, heksāns/hloroforms 5:1, hloroforms, kā pēdējo eluentu ar NH2 grupām modificēta silikagēla gadījumā izmantojot skābes (etiķskābes šķīdumu. Izpētes objekti bija divi Latvijā plaši izplatīti sūnaugi (Polytrichum commune, Dicranum polysetum, kuru sastāvā identificēja vairāk nekā 45 dažādus savienojumus, daudzi no kuriem ir ar augstu bioloģisko aktivitāti. Sūnaugu sastāvā identificēja sterolus, tokoferolus, taukskābes un citi savienojumus. Izmantotā frakcionēšanas shēma pavēra iespējas identificēt daudzus savienojumus, kuri kopējā ekstrakta hromatogrammā nebija uzrādīti, piemēram, alkānu rindas savienojumi, vairāki steroli. Līdz ar to sūnaugu lipīdu cietfāzes frakcionēšanas metode ļāva noteikt vielu klātbūtni, kuras atrodas mikrodaudzumos, bet kuras varēja ļaut izsekot brīofītu pārvērtībām bioģeoķīmiskos procesos. Ieteiktā frakcionēšanas metode ļāva arī identificēt vairākas vielu grupas, kuras varētu būt perspektīvas kā jaunas bioloģiski aktīvas vielas, kā arī palīdzēja labāk izprast briofītu metabolismu, piemēram starpsugu atšķirības un sastāva sezonālās mainības raksturu.

  7. Les aspects des frottis cervico-vaginaux chez les femmes vivants avec le VIH suivies à Thiès/Sénégal et association avec le degré d'immunodépression

    Science.gov (United States)

    Bammo, Mariama; Dioussé, Pauline; Thiam, Marietou; Diop, Madoky Maguatte; Berthe, Adama; Faye, Flugence Abdou; Diallo, Thierno Abdoul Aziz; Sarr, Fatou Seck; Dione, Haby; Toure, Papa Souleymane; Diop, Bernard Marcel; Ka, Mamadou Mortalla

    2015-01-01

    De nombreuses études ont démontré que les femmes infectées par le VIH ont un risque accru de survenue de néoplasies cervicales intra épithéliales. L'association entre les deux affections étant bidirectionnelle, l'objectif était de décrire les anomalies cervicales chez les femmes séropositives au virus de l'immunodéficience humaine (VIH), de rechercher des facteurs associés et de proposer des recommandations en termes de suivi de ces femmes. Il s'agissait d'une étude transversale, multicentrique recensant l'ensemble des frottis cervico-vaginaux (FCV) et des colposcopies des patientes infectées par le VIH entre 2012 et 2014 dans les services de dermatologie de Thiès et de Mbour. Les données étaient recueillies et analysées par le logiciel EPI Info 2012 version 3.5.4. Les tests statistiques ont été effectués avec un seuil de significativité p VIH. Un dépistage et un traitement précoces sont absolument nécessaires. PMID:26834915

  8. Sonoma County, CA, 2013 Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sonoma County Vegetation Mapping and LiDAR Consortium retained WSI to provide lidar and Orthophoto data and derived products in Sonoma County, CA. A classified LAS...

  9. CA125 in ovarian cancer

    DEFF Research Database (Denmark)

    Duffy, M J; Bonfrer, J M; Kulpa, J

    2005-01-01

    value in the detection of early ovarian cancer. At present, therefore, CA125, either alone or in combination with other modalities, cannot be recommended for screening for ovarian cancer in asymptomatic women outside the context of a randomized controlled trial. Preoperative levels in postmenopausal...... women, however, may aid the differentiation of benign and malignant pelvic masses. Serial levels during chemotherapy for ovarian cancer are useful for assessing response to treatment. Although serial monitoring following initial chemotherapy can lead to the early detection of recurrent disease......CA125 is currently the most widely used tumor marker for ovarian epithelial cancer. The aim of this article is to provide guidelines for the routine clinical use of CA125 in patients with ovarian cancer. Due to lack of sensitivity for stage I disease and lack of specificity, CA125 is of little...

  10. Cathodic behavior of molten CaCl2-CaO and CaCl2-NaCl-CaO

    Science.gov (United States)

    Wang, Shu-Lan; Wang, Wei; Li, Shi-Chao; Cao, Shan-Hui

    2010-12-01

    The cathodic behavior of molten CaCl2, CaCl2-CaO and equimolar CaCl2-NaCl-CaO was studied by cyclic voltammograms and constant potential polarization at temperatures of 1123 to 1173 K on molybdenum and titanium electrodes. The diffusion coefficient of Ca2+ (CaO) in molten CaCl2-CaO was calculated from the linear relationship between the square root of scan rate and the peak current density. The deposition potentials and the potential temperature coefficient of CaO in molten CaCl2-0.5mol%CaO and CaCl2-NaCl-0.5mol%CaO were also obtained from their cyclic voltammograms. The result shows that CaO is more easily reduced than CaCl2. The addition of NaCl in molten CaCl2-CaO induces the underpotential electrodeposition of CaO.

  11. Construye tu Música

    Directory of Open Access Journals (Sweden)

    Contreras, Antonio de

    2000-05-01

    Full Text Available En el Sistema Educativo actual la composición musical está contemplada en el tramo final de los estudios de Conservatorio, tras muchos años de adiestramiento técnico-instrumental y teórico. Por otra parte, si visitamos un Aula de Educación Plástica de un Instituto, podemos ver a los alumnos de Secundaria expresándose, canalizando su creatividad a través de colores, formas y volúmenes, sin prejuicios, con un mínimo y, en la mayoría de los casos, muy simple bagaje de conocimientos técnicos previos. Está claro que se trata de dos artes completamente diferentes, también es bien sabido y repetido hasta la saciedad el carácter abstracto de la música. Pero así y todo ¿Será posible arbitrar un modo de acercarse a la música desde dentro, desde la práctica de la composición musical de una forma directa y simple, sin necesidad de una formación musical de nivel superior, de forma parecida a como se plantea la actividad creativa en la educación plástica? ¿Será posible complementar dicha práctica con la subsiguiente interpretación de las composiciones y la crítica de las producciones ajenas del mismo nivel? Las preguntas no son, por supuesto, nuevas: ya los grandes pedagogos musicales del primer cuarto de siglo las plantearon y resolvieron cada uno a su modo. La siguiente comunicación pretende dar a conocer los resultados de una investigación en el Aula de música, realizada por el autor durante los cuatro últimos años, que parte de una propuesta original en este sentido.

  12. TuGen

    DEFF Research Database (Denmark)

    Gilling, Lasse

    An implementation of Mann's method of generating synthetic turbulence is described. A brief introduction to the method is given prior to some details on the implementation. Several tests to verify the implementation follows and finally the use of the program is described....

  13. The Basics of the Methodological/mo dal professional improvements of the physical/ sports edu ca tors from the aspect of ther actual values of life and professio nal pedagogical activity

    Directory of Open Access Journals (Sweden)

    Grujo Bjeković

    2007-05-01

    Full Text Available Whi le sol ving in cre a sed le vel of pro fes si o nal pre pa ra tion of the physi cal edu cation te ac hers, in mo dern days in cre a sed at ten tion is gi ven to in tel lec tual, mo ral, cul tu ral an pro fes si o nal in cre a se of the cre a ti ve un de pen da bi lity of the fu tu re spe ci a lists with the aim to cre a te shift from pre sently do mi nant physi cal edu ca tion, which is pri ma rily orien ted to wards the de ve lop ment of the physi cal ca pa bi li ti es for ming the per fect mo ving abi li ti es and ha bits, to de ve lop ment of the who le hu man be ing The lead ro le of the se tasks be longs to the ac me o logy, a mul ti di sci pli nary sci en ce abo ut a hu man and so ci ety. In this pa per, a ba sic ca te go rial ap pa ra tus of ac me o logy has bean de fi ned, ac cording to the le vel of it’s mo dern de ve lop ment, co ve ring pro fes si o na lism and com pe tency. To re ach de fi ned goal the fol lo wing sci en ti fi c met hods ha ve been used: analysis of met hodo lo gi cal li te ra tu re, qu e sti on na i re and mo de ling. Twenty pro fes sors of the physi cal edu ca tion with up to 10 year s and mo re as well as 20 stu dents of the Physi cal edu ca tion fa culty from the third and forth year of study ha ve been qu e sti o ned.

  14. Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR.

    Science.gov (United States)

    Ramiah, K; van Reenen, C A; Dicks, L M T

    2007-05-30

    Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA by Lactobacillus plantarum 423, grown in the presence of bile, pancreatin and at low pH, was studied by real-time PCR. Mub, MapA and EF-Tu were up-regulated in the presence of mucus, proportional to increasing concentrations. Expression of MapA was up-regulated in the presence of 3.0 g/l bile and 3.0 g/l pancreatin at pH 6.5. Similar results were recorded in the presence of 10.0 g/l bile and 10.0 g/l pancreatin at pH 6.5. Expression of Mub was down-regulated in the presence of bile and pancreatin, whilst the expression of EF-Tu and plaA remained unchanged. Expression of Mub and MapA remained unchanged at pH 4.0, whilst expression of EF-Tu and plaA were up-regulated. Expression of MapA was down-regulated in the presence of 1.0 g/l l-cysteine HCl, suggesting that the gene is regulated by transcription attenuation that involves cysteine.

  15. Supercritical fluid extraction (SFE) of 4(5)-methylimidazole (4-MeI) and 2-acetyl-4(5)-(1,2,3,4)-tetrahydroxybutyl-imidazole (THI) from ground-coffee with high-performance liquid chromatographic-electrospray mass spectrometric quantification (HPLC/ESI-MS).

    Science.gov (United States)

    Lojková, L; Klejdus, B; Moravcová, J; Kubán, V

    2006-10-01

    Two polar analytes, 4(5)-methylimidazole (4-MeI) and 2-acetyl-4(5)-(1,2,3,4)-tetrahydroxybutyl-imidazole (THI), were extracted with supercritical carbon dioxide (CO2) modified with aqueous methanol. The method was applied to a roasted coffee powder with good recovery rates. Method efficiency was compared with that of solid-phase extraction using SCX Disc cartridges and validated for spiked solid matrix. The analytes were determined using isocratic liquid chromatography-mass spectrometry (LC/MS) on an Atlantis HILIC Silica column (150 x 2.1 mm, 3 microm) with 80% methanol and 20% 0.01 mol l-1 ammonium formate as the mobile phase. The limit of quantification was around 1.5 pg for 4-MeI and 2.0 pg for THI. The linearity of the calibration curves was satisfactory as indicated by correlation coefficients of >0.999. The coefficient of variation for the intra-day and inter-day precisions was or = 98 and > or = 99% for THI and 4-MeI, respectively. Several samples of Arabica coffee from various locations and commercially available 'off-the-shelf' coffee products (Arabica/Robusta mixtures) were analysed to test the method.

  16. TU-F-18C-08: Micro-Calcification Detectability Using Spectral Breast CT Based On a Si Strip Detector

    Energy Technology Data Exchange (ETDEWEB)

    Cho, H; Ding, H; Molloi, S [University of California, Irvine, CA (United States); Barber, W; Iwanczyk, J [DxRay Inc., Northridge, CA (United States)

    2014-06-15

    Purpose: To investigate the feasibility of micro-calcification (μCa) detectability by using an energy-resolved photon-counting Si strip detector for spectral breast computed tomography (CT). Methods: A bench-top CT system was constructed using a tungsten anode x-ray source with a focal spot size of 0.8 mm and a single line 256-pixel Si strip photon counting detector with a pixel pitch of 100 μm. The slice thickness was 0.5 mm. Five different size groups of calcium carbonate grains, from 105 to 215 μm in diameter, were embedded in a cylindrical resin phantom with a diameter of 16 mm to simulate μCas. The phantoms were imaged at 65 kVp with an Entrance Skin Air Kerma (ESAK) of 1.2, 3, 6, and 8 mGy. The images were reconstructed using a standard filtered back projection (FBP) with a ramp filter. A total of 200 μCa images (5 different sizes of μCas × 4 different doses × 10 images for each setting) were combined with another 200 control images without μCas, to ultimately form 400 images for the reader study. The images were displayed in random order to three blinded observers, who were asked to give a binary score on each image regarding the presence of μCas. The μCa detectability for each image was evaluated in terms of binary decision theory metrics. The sensitivity, specificity, and accuracy were calculated to study the size and dose-dependence for μCa detectability. Additionally, the influence of the partial volume effect on the μCa detectability was investigated by simulation. Results: For a μCa larger than 140 μm in diameter, detection accuracy of above 90 % was achieved with the investigated prototype spectral CT system at ESAK of 1.2 mGy. Conclusion: The proposed Si strip detector is expected to offer superior image quality with the capability to detect μCas for low dose breast imaging.

  17. A clinical study on the efficacy of natural therapeutic factors in Băile Tuşnad for the rehabilitation of post-stroke patients

    Directory of Open Access Journals (Sweden)

    Gabriela Dogaru

    2017-02-01

    Full Text Available Introduction. Stroke is one of the main causes of morbidity and mortality worldwide. Hypotonic carbonated mineral waters in Băile Tușnad are used for their peripheral and cerebral vasodilator effects in the prophylaxis, therapy and rehabilitation of cardiovascular patients. Objectives. The aim of the clinical study was to assess the efficacy of natural therapeutic factors in Băile Tușnad for continuing the rehabilitation of post-stroke patients in a spa and climatic resort for the treatment of cardiovascular diseases. Methods. The study included 30 patients with a history of stroke, aged between 56 and 89 years, with a mean age of 69 years, at the Facility Treatment of the Tușnad Spa Complex SA, in the period April-December 2014. The clinical study was a prospective longitudinal analysis. Of all 30 patients, 50% had ischemic stroke, 43% transient cerebral ischemic attack, and 7% hemorrhagic stroke. Hemiparesis was the most frequent clinical sign, followed by coordination, balance and gait disorders. Patients attended rehabilitation treatment consisting of kinesiotherapy, carbonated mineral water baths for 15 minutes, aerotherapy for 30 minutes daily, massotherapy, performed daily for 16 days. Each patient was clinically assessed before and after treatment based on the TINETTI Balance Scale, the 10-m walk test, the Motor Assessment Scale, the BARTHEL Index, adverse reactions. Results. At the end of treatment, an improvement in the walking speed, a statistically significant improvement in the quality of gait were observed, p < 0.05. Statistically significant results p<0.05 were also obtained for balance. On the Motor Assessment Scale, by comparing the means before and after treatment with the paired T test, a statistically significant value p<0.05 was obtained. When evaluating the patients’ performance for 10 activities of daily living depending on the need for external assistance, using the Barthel Index, the value of p <0.05 was

  18. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

    Directory of Open Access Journals (Sweden)

    Shiri M

    2016-11-01

    Full Text Available Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 2School of Medicine, Artesh University of Medical Sciences, 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Department of Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, 6Toxicology Interest Group, USERN, 7Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Diazinon (DZ is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO and selenium nanoparticles (Se NPs showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double

  19. 《冥途飞脚》中的“义理与情”%The Rationalist and Emotion in Ming Tu Fei Jiao

    Institute of Scientific and Technical Information of China (English)

    赵黎慧

    2012-01-01

    Chikamatsu is Japan Edo Genroku period famous writer of doll and kabuki. Jing Yuan Xi He, Song Wei Ba Jiao and Chikamatsu are called Japan Genroku period of three literary giants. The work of Chika- matsu 《Ming Tu Fei Jiao》in Japan staged a number of the most popular work. In《Ming Tu Fei Jiao》, Zbong BingWei and Mei Chuan are in order to defend their love but to resist the morality of Edo Feudal.At the same time , they defend the credit which is set up by the Edo business community too.It is a expression that damage society common practice and business credit mood. The content of 《Ming Tu Fei Jiao》 show off the Japan Cul- ture "The Rationalist And Emotion". It is a new way for the friend exchange of Japan and China If we do un- derstand the The Rationalist And Emotion.%近松门左卫门是日本江户元禄时期有名的人形净琉璃和歌舞伎的剧作家,他与井原西鹤、松尾芭蕉一起被称为日本元禄时期的三大文学巨匠。近松门左卫门的作品《冥途飞脚》如今在日本是上演次数多、很受欢迎的作品。《冥途飞脚》中贫苦纯情的忠兵卫与梅川为了成就自己的爱情而抵抗江户封建社会道德,同时也抵抗了江户商业社会所建立起的信用组织,形成了破坏社会风气、破坏商业信用风气的一个实证。作品所表现的这种内容正是日本文化中“义理与情”矛盾与冲突的真实表现。

  20. IgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu

    Directory of Open Access Journals (Sweden)

    Flábio R Araújo

    2008-03-01

    Full Text Available Anaplasma marginale is an important vector-borne rickettsia of ruminants in tropical and subtropical regions of the world. Immunization with purified outer membranes of this organism induces protection against acute anaplasmosis. Previous studies, with proteomic and genomic approach identified 21 proteins within the outer membrane immunogen in addition to previously characterized major surface protein1a-5 (MSP1a-5. Among the newly described proteins were VirB9, VirB10, and elongation factor-Tu (EF-Tu. VirB9, VirB10 are considered part of the type IV secretion system (TFSS, which mediates secretion or cell-to-cell transfer of macromolecules, proteins, or DNA-protein complexes in Gram-negative bacteria. EF-Tu can be located in the bacterial surface, mediating bacterial attachment to host cells, or in the bacterial cytoplasm for protein synthesis. However, the roles of VirB9, VirB10, and TFSS in A. marginale have not been defined. VirB9, VirB10, and EF-Tu have not been explored as vaccine antigens. In this study, we demonstrate that sera of cattle infected with A. marginale, with homologous or heterologous isolates recognize recombinant VirB9, VirB10, and EF-Tu. IgG2 from naturally infected cattle also reacts with these proteins. Recognition of epitopes by total IgG and by IgG2 from infected cattle with A. marginale support the inclusion of these proteins in recombinant vaccines against this rickettsia.

  1. COST Action TU1206 "SUB-URBAN - A European network to improve understanding and use of the ground beneath our cities"

    Science.gov (United States)

    Campbell, Diarmad; de Beer, Johannes; Lawrence, David; van der Meulen, Michiel; Mielby, Susie; Hay, David; Scanlon, Ray; Campenhout, Ignace; Taugs, Renate; Eriksson, Ingelov

    2014-05-01

    Sustainable urbanisation is the focus of SUB-URBAN, a European Cooperation in Science and Technology (COST) Action TU1206 - A European network to improve understanding and use of the ground beneath our cities. This aims to transform relationships between experts who develop urban subsurface geoscience knowledge - principally national Geological Survey Organisations (GSOs), and those who can most benefit from it - urban decision makers, planners, practitioners and the wider research community. Under COST's Transport and Urban Development Domain, SUB-URBAN has established a network of GSOs and other researchers in over 20 countries, to draw together and evaluate collective urban geoscience research in 3D/4D characterisation, prediction and visualisation. Knowledge exchange between researchers and City-partners within 'SUB-URBAN' is already facilitating new city-scale subsurface projects, and is developing a tool-box of good-practice guidance, decision-support tools, and cost-effective methodologies that are appropriate to local needs and circumstances. These are intended to act as catalysts in the transformation of relationships between geoscientists and urban decision-makers more generally. As a result, the importance of the urban sub-surface in the sustainable development of our cities will be better appreciated, and the conflicting demands currently placed on it will be acknowledged, and resolved appropriately. Existing city-scale 3D/4D model exemplars are being developed by partners in the UK (Glasgow, London), Germany (Hamburg) and France (Paris). These draw on extensive ground investigation (10s-100s of thousands of boreholes) and other data. Model linkage enables prediction of groundwater, heat, SuDS, and engineering properties. Combined subsurface and above-ground (CityGML, BIMs) models are in preparation. These models will provide valuable tools for more holistic urban planning; identifying subsurface opportunities and saving costs by reducing uncertainty in

  2. Investigation of functional aspects of the N-terminal region of elongation factor Tu from Escherichia coli using a protein engineering approach

    DEFF Research Database (Denmark)

    Laurberg, M; Mansilla, Francisco; Clark, Brian F. C.

    1998-01-01

    importance for the factor to function properly. In this study, two lysines at positions 4 and 9 were mutated separately to alanine or glutamate. The resulting four point mutants were expressed and purified using the pGEX system. The untagged products were characterized with regard to guanine......The function of the N-terminal region of elongation factor Tu is still unexplained. Until recently, it has not been visible in electron density maps from x-ray crystallography studies, but the presence of several well conserved basic residues suggest that this part of the molecule is of structural......-nucleotide interaction, intrinsic GTPase activity, and binding of aminoacyl-tRNA (aa-tRNA). The results show that Lys9 is especially strongly involved in the association with guanine nucleotides and the binding of aa-tRNA. Also Lys4 plays a role in the association of GDP and GTP and is also of some importance in aa...

  3. Gigantes digitales. Quijote en 17000 tuits de Diego Buendía y Escribe tu propio Quijote de Belén Gache

    Directory of Open Access Journals (Sweden)

    Celia Corral Cañas

    2015-05-01

    Full Text Available To incorporate Don Quixote into the virtual environment offers many possibilities, from the literal adaptation into an electronic format, such as rewriting it on Twitter, to the allusion to the book in an avant-garde creation. Thus, among the various ways of tackling Don Quixote in Twitter, the project El Quijote en 17000 tuits by Diego Buendía, who makes a fragmented transcript in 17000 sections or tweets, stands out. Furthermore, Escribe tu propio Quijote by Belén Gache honors Cervantes’ novel with a simple interactive game that invites the reader to rethink the authorship issue within and outside the work. These two creations, born from the junction of the famous novel by Cervantes and the particularities of the new ecosystem, recover the quixotic nature to integrate it, from very different paths, into the digital universe.

  4. The site of net absorption of Ca from the intestinal tract of growing pigs and effect of phytic acid, Ca level and Ca source on Ca digestibility.

    Science.gov (United States)

    González-Vega, J Caroline; Walk, Carrie L; Liu, Yanhong; Stein, Hans H

    2014-01-01

    An experiment was conducted to test the hypothesis that the standardised digestibility of Ca in calcium carbonate and Lithothamnium calcareum Ca is not different regardless of the level of dietary Ca, and that phytic acid affects the digestibility of Ca in these two ingredients to the same degree. The objectives were to determine where in the intestinal tract Ca absorption takes place and if there are measurable quantities of basal endogenous Ca fluxes in the stomach, small intestine or large intestine. Diets contained calcium carbonate or L. calcareum Ca as the sole source of Ca, 0% or 1% phytic acid and 0.4% or 0.8% Ca. A Ca-free diet was also formulated and used to measure endogenous fluxes and losses of Ca. Nine growing pigs (initial body weight 23.8 ± 1.3 kg) were cannulated in the duodenum and in the distal ileum, and faecal, ileal and duodenal samples were collected. Duodenal endogenous fluxes of Ca were greater (p phytic acid, but decreased (p phytic acid, but may be affected by dietary Ca level depending on the Ca source. Calcium from calcium carbonate is mostly absorbed before the duodenum, but Ca from L. calcareum Ca is mostly absorbed in the jejunum and ileum.

  5. CA on CD评析

    Institute of Scientific and Technical Information of China (English)

    周秀会

    2007-01-01

    结合作者使用 CA On CD 的经验和体会,全面而具体地分析了 CA on CD 的使用功能,明确了其优点及不足之处,同时,针对这些不足之处进行了探索和尝试,提出了利用检索技巧解决这些不足之处的方法。并结合实例详细说明。

  6. The diagnostic value of joint detection of serum CA19-9, CA125 and CA242 for cholangiocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Cui Da-Peng; Han Lei; Liu Zhen-Xian; Yang He; Zhang Ying-Chun

    2016-01-01

    Objective:To study the diagnostic value of joint detection of serum CA19-9, CA125 and CA242 for cholangiocarcinoma.Methods:A total of 35 patients with cholangiocarcinoma who received surgical resection in our hospital were selected as malignant group, 30 patients with cholelithiasis who received surgical resection in our hospital during the same period were selected as benign group, serum samples were collected before surgery to determine CA19-9, CA125 and CA242 content, and cholangiocarcinoma tissue and normal bile duct tissue were collected after surgery to determine the content of proliferation and invasion molecules. Results:Serum CA19-9, CA125 and CA242 levels of malignant group were significantly higher than those of control group; PROX-1, Ki-67, Bcl-2, Bad, Gab1, LOXL2, TRPM7 and CXCL12 levels in cholangiocarcinoma tissue were higher than those in benign bile duct tissue, and E-cadherin level was lower than that in benign bile duct tissue; serum CA19-9, CA125 and CA242 levels were positively correlated with PROX-1, Ki-67, Bcl-2, Bad, Gab1, LOXL2, TRPM7 and CXCL12 levels, and negatively correlated with E-cadherin level.Conclusion:Joint detection of serum CA19-9, CA125 and CA242 can not only provide reference for the diagnosis of cholangiocarcinoma, but can also provide basis for the evaluation of proliferation, invasion and other malignant biological behaviors.

  7. Isotopic Effects on Stereodynamics for Ca+HCI, Ca+DCI, and Ca+TClReactions

    Institute of Scientific and Technical Information of China (English)

    Li-zhi Wang; Chuan-lu Yang; Jing-juan Liang; Jing Xiao; Qing-gang Zhang

    2011-01-01

    The vector correlations in Ca+HC1,Ca+DCI,and Ca+TCI reactions have been investigated by means of the quasi-classical trajectory calculations on PES constructed by means of multireference configuration interaction.The distributions of P(θr),P(Φr) and the PDDCSs of (2π/σ)(dσ00/dωt),(2π/σ)(dσ20/dωt),(2π/σ)(dσ22+/dωt),(2π/σ)(dσ21-/dωt) have been calculated based on the surface.The remarkable isotopic effects in the reactions are observed,and the mechanism which may be ascribed to different mass factors is discussed.

  8. Solar Ca II K Observations

    Science.gov (United States)

    Bertello, Luca; Pevtsov, Alexei A.; Tlatov, Andrey; Singh, Jagdev

    2016-07-01

    Some of the most important archives of past and current long-term solar synoptic observations in the resonance line of Ca II K are described here. These observations are very important for understanding the state of the solar magnetism on time scales up to several decades. The first observations of this kind began in 1904 at the Kodaikanal Observatory (India), followed by similar programs at different other locations. Regular full-disk Ca II K monitoring programs started in 1915 at the Mount Wilson Observatory (USA) and in 1917 at the National Solar Observatory of Japan. Beginning in 1919 and in 1926 regular observations were taken also at the Paris-Meudon Observatory (France) and at the "Donati solar tower telescope of the Arcetri Astrophysical Observatory in Italy, respectively. In 1926 the the Astronomical Observatory of the Coimbra University in Portugal started its own program of Ca II K observations. Although some of these programs have been terminated over the years, their data archives constitute a unique resource for studies of solar variability. In the early 1970s, the National Solar Observatory (NSO) at Sacramento Peak (USA) started a new program of daily Sun-as-a-star observations in the Ca II K line. Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility.

  9. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.

    Science.gov (United States)

    Vais, Horia; Mallilankaraman, Karthik; Mak, Don-On Daniel; Hoff, Henry; Payne, Riley; Tanis, Jessica E; Foskett, J Kevin

    2016-01-26

    The mitochondrial uniporter (MCU) is an ion channel that mediates Ca(2+) uptake into the matrix to regulate metabolism, cell death, and cytoplasmic Ca(2+) signaling. Matrix Ca(2+) concentration is similar to that in cytoplasm, despite an enormous driving force for entry, but the mechanisms that prevent mitochondrial Ca(2+) overload are unclear. Here, we show that MCU channel activity is governed by matrix Ca(2+) concentration through EMRE. Deletion or charge neutralization of its matrix-localized acidic C terminus abolishes matrix Ca(2+) inhibition of MCU Ca(2+) currents, resulting in MCU channel activation, enhanced mitochondrial Ca(2+) uptake, and constitutively elevated matrix Ca(2+) concentration. EMRE-dependent regulation of MCU channel activity requires intermembrane space-localized MICU1, MICU2, and cytoplasmic Ca(2+). Thus, mitochondria are protected from Ca(2+) depletion and Ca(2+) overload by a unique molecular complex that involves Ca(2+) sensors on both sides of the inner mitochondrial membrane, coupled through EMRE.

  10. 碳含量对TU5JP4灰铸铁冷激凸轮轴铸造弯曲变形和气孔的影响%Effect of Carbon Content on Bending Deformation and Porosity of TU5JP4 Chilled Grey Cast Iron Camshaft

    Institute of Scientific and Technical Information of China (English)

    沈保罗; 李莉; 张昊

    2012-01-01

    本文采用生产现场的大量数据,统计分析了碳含量对TU5JP4灰铸铁冷激凸轮轴弯曲变形和气孔的影响.统计数据表明,碳含量从3.4%~3.6%提高到3.6%~3.7%之间,可以较明显地降低凸轮轴的过度弯曲变形(跳动超过1mm)和气孔缺陷.用户机械加工的统计数据表明,碳含量提高后,因皮下气孔导致的废品率下降约30%.%The production data on spot have been used for statistical analysis of effect of the carbon content on bending deformation and blowhole defect of TU5JP4 chilled cast iron camshaft. It showed that increasing carbon content of the camshaft from 3.4%~3.6% to 3.6%~3.7%, has significantly reduced the bending deformation of the camshaft (radial runout more than lmm) and porosity defect. The statistics of the user showed that as the carbon content increased, the waste rate caused due to the blowhole defect decreased by about 30%.

  11. Optimization of liquid state fermentation conditions for cellulase production by Bacillus amyloliquefaciens strain Tu-115 with filter paper enzyme as index%以滤纸酶活力为指标优化解淀粉芽孢杆菌Tu-115菌株产纤维素酶液体发酵条件

    Institute of Scientific and Technical Information of China (English)

    姜军坡; 朱宝成; 王世英

    2014-01-01

    以滤纸酶活力为指标对解淀粉芽孢杆菌(Bacillus amyloliquefaciens)Tu-115菌株产纤维素酶液体发酵条件进行优化.通过单因素和正交试验相结合的方法,考察培养基中碳源、氮源、起始pH值、装瓶量、温度、接种量、发酵时间等发酵条件对产酶能力的影响.结果表明,当培养条件为葡萄糖4%、黄豆饼粉3%、培养基起始pH值4.0、接种量4%、装瓶量50 ml/250 ml,37℃发酵72 h时,发酵液中滤纸酶活力达到最高值,为3 309.90 U/ml,比优化前提高了125倍.优化后,Tu-115菌株产滤纸酶能力大大提高,达到了预期目的.

  12. Faculty: Thy Administrator's Keeper? Some Evidence

    Science.gov (United States)

    Cunningham, Brendan M.

    2009-01-01

    Colleges and universities face a principal-agent problem. There are information asymmetries over the actions chosen by administrators. Because non-profit constraints limit the financial stake of trustees there may be insufficient monitoring of administrators and, consequentially, shirking. It is conceivable that faculty will serve as "delegated…

  13. KWU-werkersklasdramas in Afrikaans (ca. 1930 - ca. 1950

    Directory of Open Access Journals (Sweden)

    J. L. Coetser

    1999-05-01

    Full Text Available GWU working class theatre in Afrikaans (ca. 1930 - ca. 1950In 1984 Elsabé Brink drew attention to plays, prose and poetry written between 1930 and 1950 in Afrikaans by members of the Garment Workers’ Union (GWU. Scholars such as Stander and Willemse (1992, Van Niekerk (1996 and Van Wyk (1995, 1997 have also referred to GWU plays. Apart from these overviews, GWU plays as such have not yet received the attention they deserve. This article presents a revaluation, initially by providing an overview of their contents, followed by an examination of cultural, economic and political influences. It is argued that - retrospectively - the GWU plays reflected a unique cultural specificity from the framework established by Sitas (1986 for more contemporary working class theatre.

  14. Ca2+ dynamics in zebrafish morphogenesis

    Directory of Open Access Journals (Sweden)

    Yusuke Tsuruwaka

    2017-01-01

    Full Text Available Intracellular calcium ion (Ca2+ signaling is heavily involved in development, as illustrated by the use of a number of Ca2+ indicators. However, continuous Ca2+ patterns during morphogenesis have not yet been studied using fluorescence resonance energy transfer to track the Ca2+ sensor. In the present study, we monitored Ca2+ levels during zebrafish morphogenesis and differentiation with yellow cameleon, YC2.12. Our results show not only clear changes in Ca2+ levels but also continuous Ca2+ patterns at 24 hpf and later periods for the first time. Serial Ca2+dynamics during early pharyngula period (Prim-5-20; 24–33 hpf was successfully observed with cameleon, which have not reported anywhere yet. In fact, high Ca2+ level occurred concurrently with hindbrain development in segmentation and pharyngula periods. Ca2+ patterns in the late gastrula through segmentation periods which were obtained with cameleon, were similar to those obtained previously with other Ca2+sensor. Our results suggested that the use of various Ca2+ sensors may lead to novel findings in studies of Ca2+ dynamics. We hope that these results will prove valuable for further research in Ca2+ signaling.

  15. Ca2+-permeable and Ca2+-impermeable AMPA receptors coexist on horizontal cells.

    Science.gov (United States)

    Huang, Shi-Yong; Liang, Pei-Ji

    2005-02-28

    Fura-2 fluorescent calcium imaging was used for analyzing the subtype of AMPA receptors in freshly dissociated horizontal cells of carp retina. Exogenous application of AMPA induced an increase of intracellular concentration of free Ca2+ ([Ca2+]i) in horizontal cells, while the [Ca2+]i increase was partly inhibited by nifedipine. The residual [Ca2+]i increase was completely eliminated by joro spider toxin-3, a blocker of Ca2+-permeable AMPA receptors. On the other hand, the application of pentobarbital, which blocked Ca2+-impermeable AMPA receptors, could also partly inhibit the increase of [Ca2+]i, implying that the application of AMPA induced the activation of both Ca2+-permeable and Ca2+-impermeable AMPA receptors and the consequent activation of voltage-gated Ca2+ channels. Taken together, these results suggested that Ca2+-permeable and Ca2+-impermeable AMPA receptors were coexpressed on horizontal cells.

  16. Lattice Dynamics of fcc Ca

    DEFF Research Database (Denmark)

    Stassis, C.; Zaretsky, J.; Misemer, D. K.;;

    1983-01-01

    to the propagation of elastic waves. The frequencies of the T1[ξξ0] branch for ξ between approximately 0.5 and 0.8 are slightly above the velocity-of-sound line determined from the low-frequency measurements. Since a similar effect has been observed in FCC Yb, it is natural to assume that the anomalous dispersion......A large single crystal of FCC Ca was grown and was used to study the lattice dynamics of this divalent metal by coherent inelastic neutron scattering. The phonon dispersion curves were measured, at room temperature, along the [ξ00], [ξξ0], [ξξξ], and [0ξ1] symmetry directions. The dispersion curves...... bear a striking resemblance to those of FCC Yb, which is also a divalent metal with an electronic band structure similar to that of Ca. In particular, the shear moduli c44 and (c11-c 12)/2 differ by a factor of 3.4, which implies that FCC Ca (like FCC Yb) is very anisotropic with regard...

  17. Continuously Tunable Ca2+ Regulation of RNA-Edited CaV1.3 Channels

    Directory of Open Access Journals (Sweden)

    Hojjat Bazzazi

    2013-10-01

    Full Text Available CaV1.3 ion channels are dominant Ca2+ portals into pacemaking neurons, residing at the epicenter of brain rhythmicity and neurodegeneration. Negative Ca2+ feedback regulation of CaV1.3 channels (CDI is therefore critical for Ca2+ homeostasis. Intriguingly, nearly half the CaV1.3 transcripts in the brain are RNA edited to reduce CDI and influence oscillatory activity. It is then mechanistically remarkable that this editing occurs precisely within an IQ domain, whose interaction with Ca2+-bound calmodulin (Ca2+/CaM is believed to induce CDI. Here, we sought the mechanism underlying the altered CDI of edited channels. Unexpectedly, editing failed to attenuate Ca2+/CaM binding. Instead, editing weakened the prebinding of Ca2+-free CaM (apoCaM to channels, which proves essential for CDI. Thus, editing might render CDI continuously tunable by fluctuations in ambient CaM, a prominent effect we substantiate in substantia nigral neurons. This adjustability of Ca2+ regulation by CaM now looms as a key element of CNS Ca2+ homeostasis.

  18. Divdaivu Ginka (Ginkgo Biloba) farmakoloģiskās īpašības un tā preparātu aprite SIA "Gulbju aptiekā"

    OpenAIRE

    Indriča, Alīna

    2014-01-01

    Divdaivu ginks (Ginkgo biloba) ir viens no vecākajiem kokiem pasaulē. Ķīnas tradicionālajā medicīnā ginka lapas un sēklas izmanto jau vismaz 5000 gadus. Standartizēts Ginkgo biloba ekstrakts satur – 24% flavonglikozīdu un 6% terpēnlaktonu. Šī darba mērķis ir veikt apkopoto datu analīzi par Ginkgo biloba preparātu apriti „Gulbju aptiekā” – Gulbenē, 2013. gada laikā, kā arī noskaidrot cilvēku informētību par Ginkgo biloba preparātu lietošanu. Darbā tika izvirzīti 4 uzdevumi: Noskaidrot, k...

  19. EFECTO DEL PROGRAMA “MUÉVETE POR TU SALUD” SOBRE LA COMPOSICIÓN CORPORAL, PRESIÓN ARTERIAL, CALIDAD DE SUEÑO Y ESTRÉS ACADÉMICO EN ESTUDIANTES RESIDENTES DE UNA UNIVERSIDAD PRIVADA CONFESIONAL

    OpenAIRE

    Salinas Arias, Saulo; Universidad Peruana Unión Filial Tarapoto; Pérez Rivera, Jessica; Castro Rodríguez, Elda

    2016-01-01

    universitaria como parte del cumplimiento de la reforma pro salud. Objetivo: Determinar laeficiencia del programa “Muévete por tu salud” en la composición corporal, presión arterial,calidad de sueño y estrés académico en los estudiantes residentes UPeU sede Tarapoto.Método: Se desarrolló el programa de actividad física denominado “Muévete por tu salud”con 79 estudiantes (F59 – M20) entre las edades de 16 y 19 años que residen en el campusuniversitario. Las variables consideradas fueron: Compo...

  20. Fine tuning of cytosolic Ca 2+ oscillations

    Science.gov (United States)

    Dupont, Geneviève; Combettes, Laurent

    2016-01-01

    Ca 2+ oscillations, a widespread mode of cell signaling, were reported in non-excitable cells for the first time more than 25 years ago. Their fundamental mechanism, based on the periodic Ca 2+ exchange between the endoplasmic reticulum and the cytoplasm, has been well characterized. However, how the kinetics of cytosolic Ca 2+ changes are related to the extent of a physiological response remains poorly understood. Here, we review data suggesting that the downstream targets of Ca 2+ are controlled not only by the frequency of Ca 2+ oscillations but also by the detailed characteristics of the oscillations, such as their duration, shape, or baseline level. Involvement of non-endoplasmic reticulum Ca 2+ stores, mainly mitochondria and the extracellular medium, participates in this fine tuning of Ca 2+ oscillations. The main characteristics of the Ca 2+ exchange fluxes with these compartments are also reviewed. PMID:27630768

  1. [Effect of polycarbophil Ca on IBS].

    Science.gov (United States)

    Mine, Tetsuya

    2006-08-01

    In this chapter, I mentioned the effect of polycarbophil Ca on IBS. IBS is classified into 3 types; diarrhea type, constipation type and combined type. Polycarbophil Ca is effective for all types of IBS.

  2. Study on need and importance of Information Literacy in Nepal: Special emphasis to students of MLisc, TU, Central Department of Library and Information Science and professionals of Kathmandu Valley

    OpenAIRE

    Shrestha, Saroja

    2005-01-01

    The present study is a study on "Need and importance of information literacy in Nepal: special emphasis to students of MLISc, Central Department of Library and Information Science, TU and professional librarians of Kathmandu". In the present context where emphasis is given more on getting the accurate information at the needed time. The professionals working as the information providers should be literate. Librarians are the primary information providers so professional librarians and student...

  3. Barnacle muscle: Ca2+, activation and mechanics.

    Science.gov (United States)

    Ashley, C C; Griffiths, P J; Lea, T J; Mulligan, I P; Palmer, R E; Simnett, S J

    1993-01-01

    In this review, aspects of the ways in which Ca2+ is transported and regulated within muscle cells have been considered, with particular reference to crustacean muscle fibres. The large size of these fibres permits easy access to the internal environment of the cell, allowing it to be altered by microinjection or microperfusion. At rest, Ca2+ is not in equilibrium across the cell membrane, it enters the cell down a steep electrochemical gradient. The free [Ca2+] at rest is maintained at a value close to 200 nM by a combination of internal buffering systems, mainly the SR, mitochondria, and the fixed and diffusible Ca(2+)-binding proteins, as well as by an energy-dependent extrusion system operating across the external cell membrane. This system relies upon the inward movement of Na+ down its own electrochemical gradient to provide the energy for the extrusion of Ca2+ ions. As a result of electrical excitation, voltage-sensitive channels for Ca2+ are activated and permit Ca2+ to enter the cell more rapidly than at rest. It has been possible to determine both the amount of Ca2+ entering by this step, and what part this externally derived Ca2+ plays in the development of force as well as in the free Ca2+ change. The latter can be determined directly by Ca(2+)-sensitive indicators introduced into the cell sarcoplasm. A combination of techniques, allowing both the total and free Ca2+ changes to be assessed during electrical excitation, has provided valuable information as to how muscle cells buffer their Ca2+ in order to regulate the extent of the change in the free Ca2+ concentration. The data indicate that the entering Ca2+ can only make a small direct contribution to the force developed by the cell. The implication here is that the major source of Ca2+ for contraction must be derived from the internal Ca2+ storage sites within the SR system, a view reinforced by caged Ca2+ methods. The ability to measure the free Ca2+ concentration changes within a single cell during

  4. CA-CA互操作研究%Research of CA-CA Interoperability

    Institute of Scientific and Technical Information of China (English)

    沈士根; 叶培松; 陈宝明

    2005-01-01

    文章介绍了国际上CA-CA之间实现互操作的信任模式,然后根据中国目前的PKI实际情况,提出了以桥CA为全局模式,局部可采用严格层次信任模式来实现各CA之间的互操作.

  5. Altered network timing in the CA3-CA1 circuit of hippocampal slices from aged mice.

    Directory of Open Access Journals (Sweden)

    Daniel J Kanak

    Full Text Available Network patterns are believed to provide unique temporal contexts for coordinating neuronal activity within and across different regions of the brain. Some of the characteristics of network patterns modeled in vitro are altered in the CA3 or CA1 subregions of hippocampal slices from aged mice. CA3-CA1 network interactions have not been examined previously. We used slices from aged and adult mice to model spontaneous sharp wave ripples and carbachol-induced gamma oscillations, and compared measures of CA3-CA1 network timing between age groups. Coherent sharp wave ripples and gamma oscillations were evident in the CA3-CA1 circuit in both age groups, but the relative timing of activity in CA1 stratum pyramidale was delayed in the aged. In another sample of aged slices, evoked Schaffer collateral responses were attenuated in CA3 (antidromic spike amplitude and CA1 (orthodromic field EPSP slope. However, the amplitude and timing of spontaneous sharp waves recorded in CA1 stratum radiatum were similar to adults. In both age groups unit activity recorded juxtacellularly from unidentified neurons in CA1 stratum pyramidale and stratum oriens was temporally modulated by CA3 ripples. However, aged neurons exhibited reduced spike probability during the early cycles of the CA3 ripple oscillation. These findings suggest that aging disrupts the coordination of patterned activity in the CA3-CA1 circuit.

  6. 《古今图书集成》医学思想研究%Study on Medical Thoughts inGu Jin Tu Shu Ji Cheng

    Institute of Scientific and Technical Information of China (English)

    杨威; 阎卫红; 余丞浩; 于峥

    2016-01-01

    Gu Jin Tu Shu Ji Cheng is a large-scale reference book in the Qing Dynasty, which is known as the ancient "Encyclopedia of China". Its content of traditional Chinese medicine is rich. Gu Jin Tu Shu Ji Cheng Yi Bu Quan Lu quoted over 120 ancient medical books covering principles of medical science, the four methods of diagnosis, clinical departments, and medical records. Other compilations and scriptures also include large amounts of medical content. The books are widely cited and precious. The contents of this book are selected finely, incorporate things of diverse nature, include large numbers of classics, and conduct clarification, with a purpose to search and retrieve; the contents also pay attention to the clinical practice, pay equal attention to the four methods of diagnosis, refer to syndrome differentiation and treatment, and include methodology and discussion, with unique views and clear logic, which can help readers’ learning.%《古今图书集成》系清代大型类书,被誉为古代“中国百科全书”,书中医学内容丰富。其中,《古今图书集成·医部全录》征引历代重要医籍120余种,涵盖医理、四诊、临床各科、医药纪事等;其他汇编、典部也有大量与医学相关的内容,征引广泛,弥足珍贵。书中对收录医学内容挑选精细,兼收并蓄,经典赅备,分门别类,便于检索阅读;重视临床实用,四诊并重,证治相参,方论俱备,观点独到,条理清晰,便于读者学习。

  7. Numerical modelling of GPR ground-matching enhancement by a chirped multilayer structure - output of cooperation within COST Action TU1208

    Science.gov (United States)

    Baghdasaryan, Hovik V.; Knyazyan, Tamara M.; Hovhannisyan, Tamara. T.; Marciniak, Marian; Pajewski, Lara

    2016-04-01

    As is well know, Ground Penetrating Radar (GPR) is an electromagnetic technique for the detection and imaging of buried objects, with resolution ranging from centimeters to few meters [1, 2]. Though this technique is mature enough and different types of GPR devices are already in use, some problems are still waiting for their solution [3]. One of them is to achieve a better matching of transmitting GPR antenna to the ground, that will increase the signal penetration depth and the signal/noise ratio at the receiving end. In the current work, a full-wave electromagnetic modelling of the interaction of a plane wave with a chirped multilayered structure on the ground is performed, via numerical simulation. The method of single expression is used, which is a suitable technique for multi-boundary problems solution [4, 5]. The considered multilayer consists of two different dielectric slabs of low and high permittivity, where the highest value of permittivity doesn't exceed the permittivity of the ground. The losses in the ground are suitably taken into account. Two types of multilayers are analysed. Numerical results are obtained for the reflectance from the structure, as well as for the distributions of electric field components and power flow density in both the considered structures and the ground. The obtained results indicate that, for a better matching with the ground, the layer closer to the ground should be the high-permittivity one. Acknowledgement This work benefited from networking activities carried out within the EU funded COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). Part of this work was developed during the Short-Term Scientific Mission COST-STSM-TU1208-25016, carried out by Prof. Baghdasaryan in the National Institute of Telecommunications in Warsaw, Poland. References [1] H. M. Jol. Ground Penetrating Radar: Theory and Applications. Elsevier, 2009. 509 pp. [2] R. Persico. Introduction to

  8. Pilsētu ilgtspējīgas attīstības plānošanas un pārvaldīšanas aspekti mūsdienās

    OpenAIRE

    Grizāns, J; Vanags, J.

    2010-01-01

    Zinātniskā raksta mērķis ir pilsētu ilgtspējīgas attīstības plānošanas un pārvaldīšanas aspektu izpēte mūsdienu urbanizācijas un globalizācijas procesu apstākļos. Nospraustā pētījuma mērķa sasniegšanai ir izmantotas šādas pētnieciskās izziņas metodes: pilsētu attīstību regulējošo normatīvo dokumentu un konceptuālo pamatnostādņu analīze, ekspertu vērtējumu izpēte, kā arī monogrāfiskās un loģiskās pieejas metodes. Pētījuma rezultātā ir izstrādāti konceptuālie priekšlikumi pilsētu sociāli ekonom...

  9. An inhibitory effect of extracellular Ca2+ on Ca2+-dependent exocytosis.

    Directory of Open Access Journals (Sweden)

    Wei Xiong

    Full Text Available AIM: Neurotransmitter release is elicited by an elevation of intracellular Ca(2+ concentration ([Ca(2+](i. The action potential triggers Ca(2+ influx through Ca(2+ channels which causes local changes of [Ca(2+](i for vesicle release. However, any direct role of extracellular Ca(2+ (besides Ca(2+ influx on Ca(2+-dependent exocytosis remains elusive. Here we set out to investigate this possibility on rat dorsal root ganglion (DRG neurons and chromaffin cells, widely used models for studying vesicle exocytosis. RESULTS: Using photolysis of caged Ca(2+ and caffeine-induced release of stored Ca(2+, we found that extracellular Ca(2+ inhibited exocytosis following moderate [Ca(2+](i rises (2-3 µM. The IC(50 for extracellular Ca(2+ inhibition of exocytosis (ECIE was 1.38 mM and a physiological reduction (∼30% of extracellular Ca(2+ concentration ([Ca(2+](o significantly increased the evoked exocytosis. At the single vesicle level, quantal size and release frequency were also altered by physiological [Ca(2+](o. The calcimimetics Mg(2+, Cd(2+, G418, and neomycin all inhibited exocytosis. The extracellular Ca(2+-sensing receptor (CaSR was not involved because specific drugs and knockdown of CaSR in DRG neurons did not affect ECIE. CONCLUSION/SIGNIFICANCE: As an extension of the classic Ca(2+ hypothesis of synaptic release, physiological levels of extracellular Ca(2+ play dual roles in evoked exocytosis by providing a source of Ca(2+ influx, and by directly regulating quantal size and release probability in neuronal cells.

  10. TU-CD-303-02: Beyond Radiation Induced Double Strand Breaks - a New Horizon for Radiation Therapy Research

    Energy Technology Data Exchange (ETDEWEB)

    Chang, S. [UNC School of Medicine (United States)

    2015-06-15

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166.

  11. 46 CFR 7.130 - Point Conception, CA to Point Sur, CA.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Point Conception, CA to Point Sur, CA. 7.130 Section 7.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY LINES Pacific Coast § 7.130 Point Conception, CA to Point Sur, CA. (a) A line drawn from...

  12. Presentation of the new Department of Drilling, Special Deep Drilling Equipment and Mining Machinery of the Institute of Drilling and Fluid Mining of TU Bergakademie Freiberg University; Vorstellung des neuen Lehrstuhls fuer Bohrtechnik, Spezialtiefbauausruestungen und Bergbaumaschinen am Institut fuer Bohrtechnik und Fluidbergbau der TU Bergakademie Freiberg

    Energy Technology Data Exchange (ETDEWEB)

    Reich, M.; Ksienzyk, F. [TU Bergakademie Freiberg (Germany)

    2007-09-13

    The paper presents the new Department of Drilling, Special Deep Drilling Equipment and Mining Machinery of the Institute of Drilling and Fluid Mining of TU Bergakademie Freiberg University. The new combination of drilling technology and mechanical engineering reflects the rapid technological development of deep drilling during the past two decades. Teaching subjects and research activities are described. Although trends are positive, there are still not enough students of drilling and fluid mining. If the rapidly increasing demand for engineers specializing in drilling technology and petroleum and natural gas production is to be met on a medium-term basis, joint efforts of industry and universities will be necessary as discussed in this paper. (orig.)

  13. Testing coral paleothermometers (B/Ca, Mg/Ca, Sr/Ca, U/Ca andδ18O) under impacts of large riverine runoff

    Institute of Scientific and Technical Information of China (English)

    CHEN Tianran; YU Kefu; ZHAO Jianxin; YAN Hongqiang; SONG Yinxian; FENG Yuexing; CHEN Tegu

    2015-01-01

    Sea surface temperature (SST) proxies including B/Ca, Mg/Ca, Sr/Ca, U/Ca andδ18O were analyzed in the skeleton of aPorites coral collected from the Zhujiang River (Pearl River) Estuary (ZRE). These geochemical proxies are influenced by river runoff and this area of the northern South China Sea is strongly affected by seasonal freshwater floods. We assessed the robustness of each SST proxy through comparison with the local instrumental SST. Coral Sr/Ca shows the highest correlation with SST variations (r2=0.59), suggesting Sr/Ca is the most robust SST proxy. In contrast, coralδ18O (r2=0.46), B/Ca (r2=0.43) and U/Ca (r2=0.41) ratios were only moderately correlated with SST variations, suggesting that they are disturbed by some other factors in addition to SST. The poor correlation (r2=0.27) between SST and Mg/Ca indicates that Mg/Ca in coral skeletons is not a simple function of SST variations. This may ultimately limit the use of Mg/Ca as a coral paleothermometer.

  14. Multiple Ca2+ sensors in secretion

    DEFF Research Database (Denmark)

    Walter, Alexander M; Groffen, Alexander J; Sørensen, Jakob Balslev;

    2011-01-01

    Regulated neurotransmitter secretion depends on Ca(2+) sensors, C2 domain proteins that associate with phospholipids and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor (SNARE) complexes to trigger release upon Ca(2+) binding. Ca(2+) sensors are thought to prevent spontaneous...... fusion at rest (clamping) and to promote fusion upon Ca(2+) activation. At least eight, often coexpressed, Ca(2+) sensors have been identified in mammals. Accumulating evidence suggests that multiple Ca(2+) sensors interact, rather than work autonomously, to produce the complex secretory response...... observed in neurons and secretory cells. In this review, we present several working models to describe how different sensors might be arranged to mediate synchronous, asynchronous and spontaneous neurotransmitter release. We discuss the scenario that different Ca(2+) sensors typically act on one shared...

  15. La satisfacción de residentes con su colonia y el programa gubernamental Tu Casa en el estado de Zacatecas

    Directory of Open Access Journals (Sweden)

    Jorge Ibarra Salazar

    2016-01-01

    Full Text Available El grado de satisfacción residencial, considerado como un indicador de nivel de vida, tiene como uno de sus componentes la satisfacción con la colonia de los individuos. En este artículo estimamos una serie de modelos para analizar el grado de satisfacción con la colonia de una muestra de hogares en el estado de Zacatecas. Las estimaciones nos permiten determinar si el programa federal de vivienda Tu Casa en ese estado, operado de 2004 a 2009, tuvo alguna influencia en el grado de satisfacción con la colonia, de acuerdo con la percepción de los residentes entrevistados. Encontramos evidencia de que el referido programa causó un efecto significativo en el grado de satisfacción del grupo de familias beneficiarias. Además de ese resultado, la estimación por mínimos cuadrados ordinarios muestra que el acceso vial, la contaminación ambiental, la percepción del valor de la casa y el tiempo de vivir en la vivienda influyen de forma significativa en el grado de satisfacción con la colonia. En los modelos estimados por probit y logit encontramos evidencia de que la seguridad pública y la posesión del inmueble también influyen en tal satisfacción.

  16. CERN Library | Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present "Si tu devais me dessiner l’Univers…" | 10 December

    CERN Multimedia

    CERN Library

    2015-01-01

    Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present Si tu devais me dessiner l’Univers… Thursday, 10 December 2015 at 3.30 p.m. at the Library, Building 52-1-052 Tea and coffee will be served at 3 p.m. In 2014, CERN launched an art competition for local primary schools. Children were asked to come up with questions about the universe, matter and working as a researcher. The initiative was so successful that it has been turned into a book that teachers can explore with their pupils, discussing the questions and discovering the answers together. The book is laid out in double-page spreads, with the child’s illustration on one page and the question and its answer opposite. The answers take the form of a short text written by Michel Spiro, the competition’s scientific advisor, together with a cultural quotation whose purpose is to highlight the close association between science, literature and philosophy. The questions include: What was there...

  17. Las revistas de tu casa: una provocación para innovar en la difusión académica

    Directory of Open Access Journals (Sweden)

    Mauricio Andión Gamboa

    2005-01-01

    Full Text Available Desde principios del siglo pasado, las universidades latinoamericanas han mostrado una vocación “extensionista”, es decir, un afán de difusión de la cultura científica y humanística más allá de los confines de las comunidades académicas. Las revistas y los libros han sido tradicionalmente la principal vía que las universidades han utilizado para difundir su producción cultural entre sus comunidades y la sociedad en general. La Galaxia de Gutenberg es sumamente extensa y, aún hoy, las universidades no han terminado de cruzarla. Las revistas universitarias son más que un medio de difusión, de hecho son proyectos académicos, la mayoría de ellos, que aglutinan grupos de interés integrados por afinidad ideológica, política, social o cultural. Para propiciar la discusión, se elaboró un video documental titulado “Las revistas de tu casa” que aborda la problemática actual de las revistas en la Universidad Autónoma Metropolitana (UAM, en su conjunto, y dentro del cambio de paradigma tecnológico.

  18. Incidence and remission of parasomnias among adolescent children in the Tucson Children’s Assessment of Sleep Apnea (TuCASA Study

    Directory of Open Access Journals (Sweden)

    Furet O

    2011-06-01

    Full Text Available Background: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group.Methods: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1 and subsequently re-studied them approximately 5 years later (Time 2. At both time points parents were asked to complete a comprehensive sleep habits questionnaire designed to assess the severity of sleep-related symptoms that included questions about enuresis (EN, sleep terrors (TR, sleep walking (SW and sleep talking (ST.Results: There were 350 children participating at Time 1 who were studied as adolescents at time 2. The mean interval between measurements was (4.6 years. The incidence of EN, TR, ST, and SW in these 10-18 year old children was 0.3%, 0.6%, 6.0% and 1.1% respectively. Remission rates were 70.8%, 100%, 64.8% and 50.0% respectively.Conclusions: The incidence rates of EN, TR, and SW were relatively low moving from childhood to adolescence while remission rates were high across all parasomnias.

  19. Tu Amigo Pepe: Evaluation of a Multi-media Marketing Campaign that Targets Young Latino Immigrant MSM with HIV Testing Messages.

    Science.gov (United States)

    Solorio, Rosa; Norton-Shelpuk, Pamela; Forehand, Mark; Montaño, Daniel; Stern, Joshua; Aguirre, Joel; Martinez, Marcos

    2016-09-01

    Latino immigrant men who have sex with men (MSM) are at risk for HIV and delayed diagnosis in the United States. This paper describes the evaluation of a pilot of the Tu Amigo Pepe, a multimedia HIV testing campaign aimed at Latino MSM in Seattle, WA particularly targeting immigrants who may not identify as gay, ages 18-30 years old. The 16-week campaign included Spanish-language radio public service announcements (PSAs), a Web site, social media outreach, a reminder system using mobile technology, print materials and a toll-free hotline. In developing the PSAs, the Integrated Behavioral Model was used as a framework to reframe negative attitudes, beliefs and norms towards HIV testing with positive ones as well as to promote self-efficacy towards HIV testing. The campaign had a significant and immediate impact on attitudes, beliefs, norms and self-efficacy towards HIV testing as well as on actual behavior, with HIV testing rates increasing over time.

  20. Tu e o de quem?" Tecendo os os com Bakhtin, Vigotski e Paulo Freire / Who do you think you are?" Commenting the wires with Bakhtin, Vigotski and Freire

    Directory of Open Access Journals (Sweden)

    Joselito Manoel de Jesus

    2010-08-01

    Full Text Available RESUMO: Este artigo analisa o enunciado "Tu é o de quem?", apontando para a responsabilidade que um filho da roça tem como partícipe do sustento da família e como essa realidade imediata constitui fios que enredam sua existência. Adota as contribuições de Bakhtin, Freire e Vigotski, evidenciando o processo de humanização como resultado das interações verbais e sociais que os indivíduos estabelecem em determinadas condições que caracterizam o espaço e o tempo em queestão inseridos e inserindo-se num processo ininterrupto. ABSTRACT:This article aims at analyzing the statement addressed to a farm child "Who do you think you are?" pointing to the fact that these children are responsible for their family income and as thisimmediate reality is intertwined by wires which build up their existence. This text is based on Bakhtin, Vygotsky and Freire, showing the process of humanization as a result of social and verbalinteractions which individuals establish under certain conditions in order to characterize the space and time in which they are inserted and inserting themselves into a never-ending process.

  1. Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength.

    Science.gov (United States)

    Barcomb, Kelsey; Buard, Isabelle; Coultrap, Steven J; Kulbe, Jacqueline R; O'Leary, Heather; Benke, Timothy A; Bayer, K Ulrich

    2014-08-01

    A hallmark feature of Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII) is generation of autonomous (Ca(2+)-independent) activity by T286 autophosphorylation. Biochemical studies have shown that "autonomous" CaMKII is ∼5-fold further stimulated by Ca(2+)/CaM, but demonstration of a physiological function for such regulation within cells has remained elusive. In this study, CaMKII-induced enhancement of synaptic strength in rat hippocampal neurons required both autonomous activity and further stimulation. Synaptic strength was decreased by CaMKIIα knockdown and rescued by reexpression, but not by mutants impaired for autonomy (T286A) or binding to NMDA-type glutamate receptor subunit 2B (GluN2B; formerly NR2B; I205K). Full rescue was seen with constitutively autonomous mutants (T286D), but only if they could be further stimulated (additional T305/306A mutation), and not with two other mutations that additionally impair Ca(2+)/CaM binding. Compared to rescue with wild-type CaMKII, the CaM-binding-impaired mutants even had reduced synaptic strength. One of these mutants (T305/306D) mimicked an inhibitory autophosphorylation of CaMKII, whereas the other one (Δstim) abolished CaM binding without introducing charged residues. Inhibitory T305/306 autophosphorylation also reduced GluN2B binding, but this effect was independent of reduced Ca(2+)/CaM binding and was not mimicked by T305/306D mutation. Thus, even autonomous CaMKII activity must be further stimulated by Ca(2+)/CaM for enhancement of synaptic strength.

  2. When is high-Ca+ microdomain required for mitochondrial Ca+ uptake?

    Science.gov (United States)

    Spät, A; Fülöp, L; Koncz, P; Szanda, G

    2009-01-01

    Ca(2+) release from IP(3)-sensitive stores in the endoplasmic reticulum (ER) induced by Ca(2+)-mobilizing agonists generates high-Ca(2+) microdomains between ER vesicles and neighbouring mitochondria. Here we present a model that describes when such microdomains are required and when submicromolar [Ca(2+)] is sufficient for mitochondrial Ca(2+) uptake. Mitochondrial Ca(2+) uptake rate in angiotensin II-stimulated H295R adrenocortical cells correlates with the proximity between ER vesicles and the mitochondrion, reflecting the uptake promoting effect of high-Ca(2+) peri-mitochondrial microdomains. Silencing or inhibition of p38 mitogen-activated protein kinase (MAPK) or inhibition of the novel isoforms of protein kinase C enhances mitochondrial Ca(2+) uptake and abolishes the positive correlation between Ca(2+) uptake and ER-mitochondrion proximity. Inhibition of protein phosphatases attenuates mitochondrial Ca(2+) uptake and also abolishes its positive correlation with ER-mitochondrion proximity. We postulate that during IP(3)-induced Ca(2+) release, Ca(2+) uptake is confined to ER-close mitochondria, because of the simultaneous activation of the protein kinases. Attenuation of Ca(2+) uptake prevents Ca(2+) overload of mitochondria and thus protects the cell against apoptosis. On the other hand, all the mitochondria accumulate Ca(2+) at a non-inhibited rate during physiological Ca(2+) influx through the plasma membrane. Membrane potential is higher in ER-distant mitochondria, providing a bigger driving force for Ca(2+) uptake. Our model explains why comparable mitochondrial Ca(2+) signals are formed in response to K(+) and angiotensin II (equipotent in respect to global cytosolic Ca(2+) signals), although only the latter generates high-Ca(2+) microdomains.

  3. In Situ Ca2+ Titration in the Fluorometric Study of Intracellular Ca2+ Binding

    Science.gov (United States)

    McMahon, Shane M.; Jackson, Meyer B.

    2014-01-01

    Imaging with Ca2+-sensitive fluorescent dye has provided a wealth of insight into the dynamics of cellular Ca2+ signaling. The spatiotemporal evolution of intracellular free Ca2+ observed in imaging experiments is shaped by binding and unbinding to cytoplasmic Ca2+ buffers, as well as the fluorescent indicator used for imaging. These factors must be taken into account in the interpretation of Ca2+ imaging data, and can be exploited to investigate endogenous Ca2+ buffer properties. Here we extended the use of Ca2+ fluorometry in the characterization of Ca2+ binding molecules within cells, building on a method of titration of intracellular Ca2+ binding sites in situ with measured amounts of Ca2+ entering through voltage-gated Ca2+ channels. We developed a systematic procedure for fitting fluorescence data acquired during a series of voltage steps to models with multiple Ca2+ binding sites. The method was tested on simulated data, and then applied to 2-photon fluorescence imaging data from rat posterior pituitary nerve terminals patch clamp-loaded with the Ca2+ indicator fluo-8. Focusing on data sets well described by a single endogenous Ca2+ buffer and dye, this method yielded estimates of the endogenous buffer concentration and Kd, the dye Kd, and the fraction of Ca2+ inaccessible cellular volume. The in situ Kd of fluo-8 thus obtained was indistinguishable from that measured in vitro. This method of calibrating Ca2+-sensitive fluorescent dyes in situ has significant advantages over previous methods. Our analysis of Ca2+ titration fluorometric data makes more effective use of the experimental data, and provides a rigorous treatment of multivariate errors and multiple Ca2+ binding species. This method offers a versatile approach to the study of endogenous Ca2+ binding molecules in their physiological milieu. PMID:25465896

  4. TU-CD-BRA-12: Coupling PET Image Restoration and Segmentation Using Variational Method with Multiple Regularizations

    Energy Technology Data Exchange (ETDEWEB)

    Li, L; Tan, S [Huazhong University of Science and Technology, Wuhan, Hubei (China); Lu, W [University of Maryland School of Medicine, Baltimore, MD (United States)

    2015-06-15

    Science Foundation of China (NNSFC), under Grant No.61375018, and Fundamental Research Funds for the Central Universities, under Grant No. 2012QN086. Wei Lu was supported in part by the National Institutes of Health (NIH) Grant No. R01 CA172638.

  5. TU-AB-303-08: GPU-Based Software Platform for Efficient Image-Guided Adaptive Radiation Therapy

    Energy Technology Data Exchange (ETDEWEB)

    Park, S; Robinson, A; McNutt, T; Wong, J; Lee, J [Johns Hopkins University, Baltimore, MD (United States); Plishker, W; Zaki, G [IGI Technologies Inc., College Park, MD (United States); Shekhar, R [IGI Technologies Inc., College Park, MD (United States); Children’s National Medical Center, Washington, D.C. (United States)

    2015-06-15

    efficient DIR, auto-segmentation, and dose computation, thus supporting an efficient ART workflow. This work was supported by NIH/NCI under grant R42CA137886.

  6. TU-AB-303-04: Characterizing CT-Derived Mass Change of Non-Tumor Pathology During Lung Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Guy, C; Weiss, E; Jan, N; Hugo, G [Virginia Commonwealth University, Richmond, VA (United States); Christensen, G [University of Iowa, Iowa City, IA (United States)

    2015-06-15

    National Cancer Institute of the National Institutes of Health under Award Number R01CA166119. The authors have no conflicts of interest.

  7. TU-C-BRE-02: A Novel, Highly Efficient and Automated Quality Assurance Tool for Modern Linear Accelerators

    Energy Technology Data Exchange (ETDEWEB)

    Goddu, S; Sun, B; Yaddanapudi, S; Kamal, G; Mutic, S [Washington University, St. Louis, MO (United States); Baltes, C; Rose, S [Varian Medical Systems iLab GmbH, Baden, Dattwil (Switzerland); Stinson, K [Varian Medical Systems, Palo Alto, CA (United States)

    2014-06-15

    for QA of TrueBeam systems and its automation makes it highly efficient for testing both geometric and dosimetric aspects of the machine. This is very important for hypo-fractionated SBRT treatments. Received support from Varian Medical Systems, Palo Alto, CA 94304-1038.

  8. TU-G-BRA-01: Assessing Radiation-Induced Reductions in Regional Lung Perfusion Following Stereotactic Radiotherapy for Lung Cancer

    Energy Technology Data Exchange (ETDEWEB)

    McGurk, R; Green, R; Lawrence, M [NC Cancer Hospital, Chapel Hill, NC (United States); Schreiber, E; Das, S; Zagar, T; Marks, L [UNC School of Medicine, Chapel Hill, NC (United States); Sheikh, A; McCartney, W; Rivera, P [University of North Carolina at Chapel Hill, Chapel Hill, NC (United States)

    2015-06-15

    Purpose: The dose-dependent nature of radiation therapy (RT)-induced lung injury following hypo-fractionated stereotactic RT is unclear. We herein report preliminary results of a prospective study assessing the magnitude of RT-induced reductions in regional lung perfusion following hypo-fractionated stereotactic RT. Methods: Four patients undergoing hypo-fractionated stereotactic lung RT (SBRT: 12 Gy x 4 fractions or 10 Gy x 5 fractions) had a pre-treatment SPECT (single-photon emission computed tomography) perfusion scan providing a 3D map of regional lung perfusion. Scans were repeated 3–6 months post-treatment. Pre- and post SPECT scans were registered to the planning CT scan (and hence the 3D dose data). Changes in regional perfusion (counts per cc on the pre-post scans) were computed in regions of the lung exposed to different doses of radiation (in 5 Gy intervals), thus defining a dose-response function. SPECT scans were internally normalized to the regions receiving <5 Gy. Results: At 3 months post-RT, the changes in perfusion are highly variable. At 6 months, there is a consistent dose-dependent reduction in regional perfusion. The average percent decline in regional perfusion was 10% at 15–20 Gy, 20% at 20–25 Gy, and 30% at 25–30 Gy representing a relatively linear dose response with an approximate 2% reduction per Gray for doses in excess of 10 Gy. There was a subtle increase in perfusion in the lung receiving <10 Gy. Conclusion: Hypo-fractionated stereotactic RT appears to cause a dose-dependent reduction in regional lung perfusion. There appears to be a threshold effect with no apparent perfusion loss at doses <10 Gy, though this might be in part due to the normalization technique used. Additional data is needed from a larger number of patients to better assess this issue. This sort of data can be used to assist optimizing RT treatment plans that minimize the risk of lung injury. Partly supported by the NIH (CA69579) and the Lance Armstrong

  9. TU-EF-204-12: Quantitative Evaluation of Spectral Detector CT Using Virtual Monochromatic Images: Initial Results

    Energy Technology Data Exchange (ETDEWEB)

    Duan, X; Guild, J [UT Southwestern Medical Center, Dallas, TX (United States); Arbique, G; Anderson, J [UT Southwestern Medical Ctr at Dallas, Dallas, TX (United States); Dhanantwari, A [Philips Healthcare, Highland Heights, OH (United States); Yagil, Y [Philips Medical Systems, Haifa (Israel)

    2015-06-15

    Purpose To evaluate the image quality and spectral information of a spectral detector CT (SDCT) scanner using virtual monochromatic (VM) energy images. Methods The SDCT scanner (Philips Healthcare) was equipped with a dual-layer detector and spectral iterative reconstruction (IR), which generates conventional 80–140 kV polychromatic energy (PE) CT images using both detector layers, PE images from the low-energy (upper) and high-energy (lower) detector layers and VM images. A solid water phantom with iodine (2.0–20.0 mg I/ml) and calcium (50.0–600.0 mg Ca/ml) rod inserts was used to evaluate effective energy estimate (EEE) and iodine contrast to noise ratio (CNR). The EEE corresponding to an insert CT number in a PE image was calculated from a CT number fit to the VM image set. Since PE image is prone to beam-hardening artifact EEE may underestimate the actual energy separation from two layers of the detector. A 30-cm-diameter water phantom was used to evaluate noise power spectrum (NPS). The phantoms were scanned at 120 and 140 kV with the same CTDIvol. Results The CT number difference for contrast inserts in VM images (50–150 keV) was 1.3±6% between 120 and 140 kV scans. The difference of EEE calculated from low- and high-energy detector images was 11.5 and 16.7 keV for 120 and 140 kV scans, respectively. The differences calculated from 140 and 100 kV conventional PE images were 12.8, and 20.1 keV from 140 and 80 kV conventional PE images. The iodine CNR increased monotonically with decreased keV. Compared to conventional PE images, the peak of NPS curves from VM images were shifted to lower frequency. Conclusion The EEE results indicates that SDCT at 120 and 140 kV may have energy separation comparable to 100/140 kV and 80/140 kV dual-kV imaging. The effects of IR on CNR and NPS require further investigation for SDCT. Author YY and AD are Philips Healthcare employees.

  10. Involvement of Ca2+/CaM in the signal transduction of acetylcholine regulating stomatal movement

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    It has been known that the neurotransmitter acetylcholine (ACh) also exists in plants and is able to regulate the movement of stomata. In another aspect, Ca2+/CaM as the second messengers have a critical role of signal transduction in stomatal guard-cell. Here we showed that Ca2+/CaM were also involved in theACh regulated stomatal movement. In the medium containing Ca2+, the Ca2+ channel blockers (NIF and Ver) and CaM inhibitors (TFP and W7) could neutralize the ACh induced stomatal opening, however, they are ineffective in the medium containing K+. Those results indicated that Ca2+/CaM were involved in the signal transduction pathway of ACh regulating stomatal movement.

  11. Influence of NaCl-CaCl2 on Decomposing REPO4 with CaO

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The influence of NaCl-CaCl2 on thermal decomposition of REPO4 (RE: Ce, La, Nd, Th) with CaO was studied.The heat decomposing process of REPO4 was tested with TG-DTA experiments.The results showed that the decomposition temperature of REPO4 with CaO was reduced because of adding NaCl-CaCl2 mixture (NaCl:CaCl2=1:1).The influence of the addition of NaCl-CaCl2, roasting temperature and roasting time on decomposition ratio of REPO4 with CaO was studied.The results showed that the decomposition ratio of REPO4with CaO was 79% when the addition percentage of NaCl-CaCl2 was 10%, the roasting temperature was 750℃, and the roasting time was 1 h.

  12. Ca2+ cycling in heart cells from ground squirrels: adaptive strategies for intracellular Ca2+ homeostasis.

    Directory of Open Access Journals (Sweden)

    Xiao-Chen Li

    Full Text Available Heart tissues from hibernating mammals, such as ground squirrels, are able to endure hypothermia, hypoxia and other extreme insulting factors that are fatal for human and nonhibernating mammals. This study was designed to understand adaptive mechanisms involved in intracellular Ca(2+ homeostasis in cardiomyocytes from the mammalian hibernator, ground squirrel, compared to rat. Electrophysiological and confocal imaging experiments showed that the voltage-dependence of L-type Ca(2+ current (I(Ca was shifted to higher potentials in ventricular myocytes from ground squirrels vs. rats. The elevated threshold of I(Ca did not compromise the Ca(2+-induced Ca(2+ release, because a higher depolarization rate and a longer duration of action potential compensated the voltage shift of I(Ca. Both the caffeine-sensitive and caffeine-resistant components of cytosolic Ca(2+ removal were more rapid in ground squirrels. Ca(2+ sparks in ground squirrels exhibited larger amplitude/size and much lower frequency than in rats. Due to the high I(Ca threshold, low SR Ca(2+ leak and rapid cytosolic Ca(2+ clearance, heart cells from ground squirrels exhibited better capability in maintaining intracellular Ca(2+ homeostasis than those from rats and other nonhibernating mammals. These findings not only reveal adaptive mechanisms of hibernation, but also provide novel strategies against Ca(2+ overload-related heart diseases.

  13. γCaMKII shuttles Ca²⁺/CaM to the nucleus to trigger CREB phosphorylation and gene expression.

    Science.gov (United States)

    Ma, Huan; Groth, Rachel D; Cohen, Samuel M; Emery, John F; Li, Boxing; Hoedt, Esthelle; Zhang, Guoan; Neubert, Thomas A; Tsien, Richard W

    2014-10-09

    Activity-dependent CREB phosphorylation and gene expression are critical for long-term neuronal plasticity. Local signaling at CaV1 channels triggers these events, but how information is relayed onward to the nucleus remains unclear. Here, we report a mechanism that mediates long-distance communication within cells: a shuttle that transports Ca(2+)/calmodulin from the surface membrane to the nucleus. We show that the shuttle protein is γCaMKII, its phosphorylation at Thr287 by βCaMKII protects the Ca(2+)/CaM signal, and CaN triggers its nuclear translocation. Both βCaMKII and CaN act in close proximity to CaV1 channels, supporting their dominance, whereas γCaMKII operates as a carrier, not as a kinase. Upon arrival within the nucleus, Ca(2+)/CaM activates CaMKK and its substrate CaMKIV, the CREB kinase. This mechanism resolves long-standing puzzles about CaM/CaMK-dependent signaling to the nucleus. The significance of the mechanism is emphasized by dysregulation of CaV1, γCaMKII, βCaMKII, and CaN in multiple neuropsychiatric disorders.

  14. Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.

    Science.gov (United States)

    Niwa, Fumihiro; Sakuragi, Shigeo; Kobayashi, Ayana; Takagi, Shin; Oda, Yoichi; Bannai, Hiroko; Mikoshiba, Katsuhiko

    2016-10-07

    Calcium (Ca(2+)) is a versatile intracellular second messenger that operates in various signaling pathways leading to multiple biological outputs. The diversity of spatiotemporal patterns of Ca(2+) signals, generated by the coordination of Ca(2+) influx from the extracellular space and Ca(2+) release from the intracellular Ca(2+) store the endoplasmic reticulum (ER), is considered to underlie the diversity of biological outputs caused by a single signaling molecule. However, such Ca(2+) signaling diversity has not been well described because of technical limitations. Here, we describe a new method to report Ca(2+) signals at subcellular resolution. We report that OER-GCaMP6f, a genetically encoded Ca(2+) indicator (GECI) targeted to the outer ER membrane, can monitor Ca(2+) release from the ER at higher spatiotemporal resolution than conventional GCaMP6f. OER-GCaMP6f was used for in vivo Ca(2+) imaging of C. elegans. We also found that the spontaneous Ca(2+) elevation in cultured astrocytes reported by OER-GCaMP6f showed a distinct spatiotemporal pattern from that monitored by plasma membrane-targeted GCaMP6f (Lck-GCaMP6f); less frequent Ca(2+) signal was detected by OER-GCaMP6f, in spite of the fact that Ca(2+) release from the ER plays important roles in astrocytes. These findings suggest that targeting of GECIs to the ER outer membrane enables sensitive detection of Ca(2+) release from the ER at subcellular resolution, avoiding the diffusion of GECI and Ca(2+). Our results indicate that Ca(2+) imaging with OER-GCaMP6f in combination with Lck-GCaMP6f can contribute to describing the diversity of Ca(2+) signals, by enabling dissection of Ca(2+) signals at subcellular resolution.

  15. Ca2+ uptake by the endoplasmic reticulum Ca2+-ATPase in rat microvascular endothelial cells.

    Science.gov (United States)

    Moccia, Francesco; Berra-Romani, Roberto; Baruffi, Silvana; Spaggiari, Santina; Signorelli, Silvia; Castelli, Loretta; Magistretti, Jacopo; Taglietti, Vanni; Tanzi, Franco

    2002-01-01

    In non-excitable cells, many agonists increase the intracellular Ca(2+) concentration ([Ca(2+)](i)) by inducing an inositol 1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release from the intracellular stores. Ca(2+) influx from the extracellular medium may then sustain the Ca(2+) signal. [Ca(2+)](i) recovers its resting level as a consequence of Ca(2+)-removing mechanisms, i.e. plasma-membrane Ca(2+)-ATPase (PMCA) pump, Na(+)/Ca(2+) exchanger (NCX) and sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump. In a study performed in pancreatic acinar cells, evidence has been provided suggesting that, during the decay phase of the agonist-evoked Ca(2+) transients, the Ca(2+) concentration within the intracellular stores remains essentially constant [Mogami, Tepikin and Petersen (1998) EMBO J. 17, 435-442]. It was therefore hypothesized that, in such a situation, intracellular Ca(2+) is not only picked up by the SERCA pump, but is also newly released through IP(3)-sensitive Ca(2+) channels, with the balance between these two processes being approximately null. The main aim of the present work was to test this hypothesis by a different experimental approach. Using cardiac microvascular endothelial cells, we found that inhibition of the SERCA pump has no effect on the time course of agonist-evoked Ca(2+) transients. This result was not due to a low capacity of the SERCA pump since, after agonist removal, this pump proved to be very powerful in clearing the excess of intracellular Ca(2+). We showed further that: (i) in order to avoid a rapid removal of Ca(2+) by the SERCA pump, continuous IP(3) production appears to be required throughout all of the decay phase of the Ca(2+) transient; and (ii) Ca(2+) picked up by the SERCA pump can be fully and immediately released by agonist application. All these results support the model of Mogami, Tepikin and Petersen [(1998) EMBO J. 17, 435-442]. Since the SERCA pump did not appear to be involved in shaping the decay phase of the

  16. Localized Calcineurin Confers Ca2+-Dependent Inactivation Upon Neuronal L-Type Ca2+ Channels

    OpenAIRE

    2012-01-01

    Excitation-driven entry of Ca2+ through L-type voltage-gated Ca2+ channels controls gene expression in neurons and a variety of fundamental activities in other kinds of excitable cells. The probability of opening of CaV1.2 L-type channels is subject to pronounced enhancement by cAMP-dependent protein kinase (PKA), which is scaffolded to CaV1.2 channels by A-kinase anchoring proteins (AKAPs). CaV1.2 channels also undergo negative autoregulation via Ca2+-dependent inactivation (CDI), which stro...

  17. Interlamellar CA1 network in the hippocampus

    OpenAIRE

    Yang, Sunggu; Yang, Sungchil; Moreira, Thais; Hoffman, Gloria; Carlson, Greg C.; Bender, Kevin J.; Alger, Bradley E.; Tang, Cha-Min

    2014-01-01

    It has generally been thought that CA1 cells form only negligible connections with each other along the longitudinal axis of the hippocampus. But if CA1 cells were interconnected in an effective autoassociational network, this information would add a critical new dimension to our understanding of cellular processing within this structure. Here, we report the existence of a well-organized, longitudinally projecting synaptic network among CA1 pyramidal neurons. We further show that synapses of ...

  18. Glutamate excitotoxicity and Ca2+-regulation of respiration: Role of the Ca2+ activated mitochondrial transporters (CaMCs).

    Science.gov (United States)

    Rueda, Carlos B; Llorente-Folch, Irene; Traba, Javier; Amigo, Ignacio; Gonzalez-Sanchez, Paloma; Contreras, Laura; Juaristi, Inés; Martinez-Valero, Paula; Pardo, Beatriz; Del Arco, Araceli; Satrustegui, Jorgina

    2016-08-01

    Glutamate elicits Ca(2+) signals and workloads that regulate neuronal fate both in physiological and pathological circumstances. Oxidative phosphorylation is required in order to respond to the metabolic challenge caused by glutamate. In response to physiological glutamate signals, cytosolic Ca(2+) activates respiration by stimulation of the NADH malate-aspartate shuttle through Ca(2+)-binding to the mitochondrial aspartate/glutamate carrier (Aralar/AGC1/Slc25a12), and by stimulation of adenine nucleotide uptake through Ca(2+) binding to the mitochondrial ATP-Mg/Pi carrier (SCaMC-3/Slc25a23). In addition, after Ca(2+) entry into the matrix through the mitochondrial Ca(2+) uniporter (MCU), it activates mitochondrial dehydrogenases. In response to pathological glutamate stimulation during excitotoxicity, Ca(2+) overload, reactive oxygen species (ROS), mitochondrial dysfunction and delayed Ca(2+) deregulation (DCD) lead to neuronal death. Glutamate-induced respiratory stimulation is rapidly inactivated through a mechanism involving Poly (ADP-ribose) Polymerase-1 (PARP-1) activation, consumption of cytosolic NAD(+), a decrease in matrix ATP and restricted substrate supply. Glutamate-induced Ca(2+)-activation of SCaMC-3 imports adenine nucleotides into mitochondria, counteracting the depletion of matrix ATP and the impaired respiration, while Aralar-dependent lactate metabolism prevents substrate exhaustion. A second mechanism induced by excitotoxic glutamate is permeability transition pore (PTP) opening, which critically depends on ROS production and matrix Ca(2+) entry through the MCU. By increasing matrix content of adenine nucleotides, SCaMC-3 activity protects against glutamate-induced PTP opening and lowers matrix free Ca(2+), resulting in protracted appearance of DCD and protection against excitotoxicity in vitro and in vivo, while the lack of lactate protection during in vivo excitotoxicity explains increased vulnerability to kainite-induced toxicity in Aralar

  19. The initial 41Ca/40Ca ratios in two type A Ca-Al-rich inclusions: Implications for the origin of short-lived 41Ca

    Science.gov (United States)

    Liu, Ming-Chang

    2017-03-01

    This paper reports new 41Ca-41K isotopic data for two Type A CAIs, NWA 3118 #1Nb (Compact Type A) and Vigarano 3138 F8 (Fluffy Type A), from reduced CV3 chondrites. The NWA CAI is found to have carried live 41Ca at the level of (4.6 ± 1.9) ×10-9 , consistent with the proposed Solar System initial 41Ca /40Ca = 4.2 ×10-9 by Liu et al. (2012a). On the other hand, the Vigarano CAI does not have resolvable radiogenic 41K excesses that can be attributed to the decay of 41Ca. Combined with the 26Al data that have been reported for these two CAIs, we infer that the 41Ca distribution was not homogeneous when 26Al was widespread at the canonical level of 26Al /27Al = 5.2 ×10-5 . Such a 41Ca heterogeneity can be understood under two astrophysical contexts: in situ charged particle irradiation by the protoSun in the solar nebula that had inherited some baseline 10Be abundance from the molecular cloud, and Solar System formation in a molecular cloud enriched in 26Al and 41Ca contaminated by massive star winds. That said, more high quality 41Ca data are still needed to better understand the origin of this radionuclide.

  20. „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego

    Directory of Open Access Journals (Sweden)

    Bartosz Cemborowski

    2015-12-01

    Full Text Available On why have the dialects of the Province of Greater Poland survived (an example, the language of Międzychodzki district inhabitants The aim of this article is to respond to the question: Why have the dialects of the Province of Greater Poland survived while the dialects from other parts of Poland are in decline? The analytical material relates to the statements of the users of this dialect who live in the western parts of Greater Poland (Międzychodzki district. It was collected during fieldwork in the villages of the Międzychodzki district between 2012 and 2015. The research methodology was based on autobiographical interviews, questionnaires and participant observations. Three important factors which contributed to the survival of the dialects of Greater Poland are: the history of this region, the identity of its people and the fashion for dialect use among the younger generation of Greater Poland’s inhabitants.   „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego Celem artykułu jest odpowiedź na pytanie, dlaczego gwary wielkopolskie przetrwały, podczas gdy w innych regionach Polski zauważalna jest tendencja do ich zaniku. Materiałem wykorzystanym w analizie są wypowiedzi mieszkańców wsi powiatu międzychodzkiego, którzy posługują się gwarą. Materiał został zebrany podczas badań terenowych w latach 2012–2015 za pomocą takich metod, jak wywiad autobiograficzny, ankiety i obserwacja uczestnicząca. Trzema istotnymi czynnikami, które wpłynęły na to, że gwary przetrwały w języku respondentów, są: historia, identyfikacja i narastająca wśród młodego pokolenia moda na mówienie gwarą.

  1. GPR surveying of transport infrastructures and buildings; underground utility and void sensing - ongoing activities in Working Group 2 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Plati, Christina; Derobert, Xavier

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 2 'GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Four Working Groups (WGs) carry out the research activities. WG1 focuses on the development of innovative GPR equipment dedicated for civil engineering applications. WG2 deals with the development of guidelines and protocols for the surveying, through the use of a GPR system, of transport infrastructure and buildings, as well as for the sensing of utilities and voids. WG3 deals with the development of electromagnetic forward and inverse scattering methods, for the characterization of GPR scenarios, as well as with data- processing algorithms for the elaboration of the data collected during GPR surveys. WG4 is concerned with the use of GPR in fields different from the civil engineering, as well as with the integration of GPR with other non-destructive testing techniques. Each WG includes several Projects. WG2 includes five Projects. Project 2.1 focuses on outlining 'Innovative inspection procedures for effective GPR surveying of critical transport infrastructures (pavements, bridges and tunnels).' Project 2.2 is concerned with the development of 'Innovative inspection procedures for effective GPR surveying of buildings.' Project 2.3 deals with identifying 'Innovative inspection procedures for effective GPR sensing and mapping of underground utilities and voids, with a focus to urban

  2. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.

    Science.gov (United States)

    Ohkura, Masamichi; Sasaki, Takuya; Sadakari, Junko; Gengyo-Ando, Keiko; Kagawa-Nagamura, Yuko; Kobayashi, Chiaki; Ikegaya, Yuji; Nakai, Junichi

    2012-01-01

    Imaging the activities of individual neurons with genetically encoded Ca(2+) indicators (GECIs) is a promising method for understanding neuronal network functions. Here, we report GECIs with improved neuronal Ca(2+) signal detectability, termed G-CaMP6 and G-CaMP8. Compared to a series of existing G-CaMPs, G-CaMP6 showed fairly high sensitivity and rapid kinetics, both of which are suitable properties for detecting subtle and fast neuronal activities. G-CaMP8 showed a greater signal (F(max)/F(min) = 38) than G-CaMP6 and demonstrated kinetics similar to those of G-CaMP6. Both GECIs could detect individual spikes from pyramidal neurons of cultured hippocampal slices or acute cortical slices with 100% detection rates, demonstrating their superior performance to existing GECIs. Because G-CaMP6 showed a higher sensitivity and brighter baseline fluorescence than G-CaMP8 in a cellular environment, we applied G-CaMP6 for Ca(2+) imaging of dendritic spines, the putative postsynaptic sites. By expressing a G-CaMP6-actin fusion protein for the spines in hippocampal CA3 pyramidal neurons and electrically stimulating the granule cells of the dentate gyrus, which innervate CA3 pyramidal neurons, we found that sub-threshold stimulation triggered small Ca(2+) responses in a limited number of spines with a low response rate in active spines, whereas supra-threshold stimulation triggered large fluorescence responses in virtually all of the spines with a 100% activity rate.

  3. Supralinear dendritic Ca(2+) signalling in young developing CA1 pyramidal cells.

    Science.gov (United States)

    Pohle, Jörg; Bischofberger, Josef

    2014-11-15

    Although Ca(2+) is critically important in activity-dependent neuronal development, not much is known about the regulation of dendritic Ca(2+) signals in developing neurons. Here, we used ratiometric Ca(2+) imaging to investigate dendritic Ca(2+) signalling in rat hippocampal pyramidal cells during the first 1-4 weeks of postnatal development. We show that active dendritic backpropagation of Nav channel-dependent action potentials (APs) evoked already large dendritic Ca(2+) transients in animals aged 1 week with amplitudes of ∼150 nm, similar to the amplitudes of ∼160 nM seen in animals aged 4 weeks. Although the AP-evoked dendritic Ca(2+) load increased about four times during the first 4 weeks, the peak amplitude of free Ca(2+) concentration was balanced by a four-fold increase in Ca(2+) buffer capacity κs (∼70 vs. ∼280). Furthermore, Ca(2+) extrusion rates increased with postnatal development, leading to a slower decay time course (∼0.2 s vs. ∼0.1 s) and more effective temporal summation of Ca(2+) signals in young cells. Most importantly, during prolonged theta-burst stimulation dendritic Ca(2+) signals were up to three times larger in cells at 1 week than at 4 weeks of age and much larger than predicted by linear summation, which is attributable to an activity-dependent slow-down of Ca(2+) extrusion. As Ca(2+) influx is four-fold smaller in young cells, the larger Ca(2+) signals are generated using four times less ATP consumption. Taken together, the data suggest that active backpropagations regulate dendritic Ca(2+) signals during early postnatal development. Remarkably, during prolonged AP firing, Ca(2+) signals are several times larger in young than in mature cells as a result of activity-dependent regulation of Ca(2+) extrusion rates.

  4. Neural Signals Related to Outcome Evaluation Are Stronger in CA1 than CA3.

    Science.gov (United States)

    Lee, Sung-Hyun; Huh, Namjung; Lee, Jong Won; Ghim, Jeong-Wook; Lee, Inah; Jung, Min W

    2017-01-01

    We have shown previously that CA1 conveys significant neural signals necessary to update value of the chosen target, namely chosen value and reward signals. To better understand hippocampal neural processes related to valuation, we compared chosen value- and reward-related neural activity between the CA3 and CA1 regions. Single units were recorded with tetrodes from the dorsal CA3 and CA1 regions of rats performing a dynamic foraging task, and chosen value- and reward-related neural activity was estimated using a reinforcement learning model and multiple regression analyses. Neural signals for chosen value and reward converged in both CA3 and CA1 when a trial outcome was revealed. However, these neural signals were stronger in CA1 than CA3. Consequently, neural signals for reward prediction error and updated chosen value were stronger in CA1 than CA3. Together with our previous finding that CA1 conveys stronger value signals than the subiculum, our results raise the possibility that CA1 might play a particularly important role among hippocampal subregions in evaluating experienced events.

  5. EMRE Is a Matrix Ca2+ Sensor that Governs Gatekeeping of the Mitochondrial Ca2+ Uniporter

    Directory of Open Access Journals (Sweden)

    Horia Vais

    2016-01-01

    Full Text Available The mitochondrial uniporter (MCU is an ion channel that mediates Ca2+ uptake into the matrix to regulate metabolism, cell death, and cytoplasmic Ca2+ signaling. Matrix Ca2+ concentration is similar to that in cytoplasm, despite an enormous driving force for entry, but the mechanisms that prevent mitochondrial Ca2+ overload are unclear. Here, we show that MCU channel activity is governed by matrix Ca2+ concentration through EMRE. Deletion or charge neutralization of its matrix-localized acidic C terminus abolishes matrix Ca2+ inhibition of MCU Ca2+ currents, resulting in MCU channel activation, enhanced mitochondrial Ca2+ uptake, and constitutively elevated matrix Ca2+ concentration. EMRE-dependent regulation of MCU channel activity requires intermembrane space-localized MICU1, MICU2, and cytoplasmic Ca2+. Thus, mitochondria are protected from Ca2+ depletion and Ca2+ overload by a unique molecular complex that involves Ca2+ sensors on both sides of the inner mitochondrial membrane, coupled through EMRE.

  6. CaV1.1: The atypical prototypical voltage-gated Ca2+ channel

    Science.gov (United States)

    Bannister, Roger A.; Beam, Kurt G.

    2012-01-01

    CaV1.1 is the prototype for the other nine known CaV channel isoforms, yet it has functional properties that make it truly atypical of this group. Specifically, CaV1.1 is expressed solely in skeletal muscle where it serves multiple purposes; it is the voltage sensor for excitation-contraction (EC) coupling and it is an L-type Ca2+ channel which contributes to a form of activity-dependent Ca2+ entry that has been termed Excitation-Coupled Ca2+ Entry (ECCE). The ability of CaV1.1 to serve as voltage-sensor for EC coupling appears to be unique amongst CaV channels, whereas the physiological role of its more conventional function as a Ca2+ channel has been a matter of uncertainty for nearly 50 years. In this chapter, we discuss how CaV1.1 supports EC coupling, the possible relevance of Ca2+ entry through CaV1.1 and how alterations of CaV1.1 function can have pathophysiological consequences. PMID:22982493

  7. Biphasic decay of the Ca transient results from increased sarcoplasmic reticulum Ca leak

    Science.gov (United States)

    Sankaranarayanan, Rajiv; Li, Yatong; Greensmith, David J.; Eisner, David A.

    2016-01-01

    Key points Ca leak from the sarcoplasmic reticulum through the ryanodine receptor (RyR) reduces the amplitude of the Ca transient and slows its rate of decay.In the presence of β‐adrenergic stimulation, RyR‐mediated Ca leak produces a biphasic decay of the Ca transient with a fast early phase and a slow late phase.Two forms of Ca leak have been studied, Ca‐sensitising (induced by caffeine) and non‐sensitising (induced by ryanodine) and both induce biphasic decay of the Ca transient.Only Ca‐sensitising leak can be reversed by traditional RyR inhibitors such as tetracaine.Ca leak can also induce Ca waves. At low levels of leak, waves occur. As leak is increased, first biphasic decay and then slowed monophasic decay is seen. The level of leak has major effects on the shape of the Ca transient. Abstract In heart failure, a reduction in Ca transient amplitude and contractile dysfunction can by caused by Ca leak through the sarcoplasmic reticulum (SR) Ca channel (ryanodine receptor, RyR) and/or decreased activity of the SR Ca ATPase (SERCA). We have characterised the effects of two forms of Ca leak (Ca‐sensitising and non‐sensitising) on calcium cycling and compared with those of SERCA inhibition. We measured [Ca2+]i with fluo‐3 in voltage‐clamped rat ventricular myocytes. Increasing SR leak with either caffeine (to sensitise the RyR to Ca activation) or ryanodine (non‐sensitising) had similar effects to SERCA inhibition: decreased systolic [Ca2+]i, increased diastolic [Ca2+]i and slowed decay. However, in the presence of isoproterenol, leak produced a biphasic decay of the Ca transient in the majority of cells while SERCA inhibition produced monophasic decay. Tetracaine reversed the effects of caffeine but not of ryanodine. When caffeine (1 mmol l−1) was added to a cell which displayed Ca waves, the wave frequency initially increased before waves disappeared and biphasic decay developed. Eventually (at higher caffeine concentrations), the

  8. Demographics, treatment and outcomes of atrial fibrillation in a developing country: the population-based TuRkish Atrial Fibrillation (TRAF) cohort.

    Science.gov (United States)

    Yavuz, Bünyamin; Ata, Naim; Oto, Emre; Katircioglu-Öztürk, Deniz; Aytemir, Kudret; Evranos, Banu; Koselerli, Rasim; Ertugay, Emre; Burkan, Abdulkadir; Ertugay, Emrah; Gale, Christ P; Camm, A John; Oto, Ali

    2017-05-01

    Although atrial fibrillation (AF) is increasingly common in developed countries, there is limited information regarding its demographics, co-morbidities, treatments and outcomes in the developing countries. We present the profile of the TuRkish Atrial Fibrillation (TRAF) cohort which provides real-life data about prevalence, incidence, co-morbidities, treatment, healthcare utilization and outcomes associated with AF. The TRAF cohort was extracted from MEDULA, a health insurance database linking hospitals, general practitioners, pharmacies and outpatient clinics for almost 100% of the inhabitants of the country. The cohort includes 507 136 individuals with AF between 2008 and 2012 aged >18 years who survived the first 30 days following diagnosis. Of 507 136 subjects, there were 423 109 (83.4%) with non-valvular AF and 84 027 (16.6%) with valvular AF. The prevalence was 0.80% in non-valvular AF and 0.28% in valvular AF; in 2012 the incidence of non-valvular AF (0.17%) was higher than valvular AF (0.04%). All-cause mortality was 19.19% (97 368) and 11.47% (58 161) at 1-year after diagnosis of AF. There were 35 707 (7.04%) ischaemic stroke/TIA/thromboembolism at baseline and 34 871 (6.87%) during follow-up; 11 472 (2.26%) major haemorrhages at baseline and 10 183 (2.01%) during follow-up, and 44 116 (8.69%) hospitalizations during the follow-up. The TRAF cohort is the first population-based, whole-country cohort of AF epidemiology, quality of care and outcomes. It provides a unique opportunity to study the patterns, causes and impact of treatments on the incidence and outcomes of AF in a developing country.

  9. Tu Salud ¡Sí Cuenta! Your Health Matters! A Community-wide Campaign in a Hispanic Border Community in Texas.

    Science.gov (United States)

    Heredia, Natalia I; Lee, MinJae; Mitchell-Bennett, Lisa; Reininger, Belinda M

    2017-08-14

    To evaluate a community-wide campaign, Tu Salud ¡Si Cuenta! (TSSC), in improving eating behaviors and anthropometric outcomes in Hispanic border communities. A quasi-experimental study with matched intervention and comparison communities. Cross-sectional assessments with randomly sampled adults, examined by actual exposure and site (unexposed intervention, exposed intervention, and unexposed comparison). Predominately Mexican Americans located in Brownsville, TX (intervention) and Laredo, TX (control). The TSSC campaign included television and radio segments, community health worker discussions, and newsletters delivered in Brownsville from 2005 to 2010. Healthy and unhealthy eating indices and average hip and waist circumferences. Univariable and multivariable regression models. The sample (n = 799; 400 comparison and 399 intervention) was 98% of Mexican origin; 54% had completed grade 9 or higher. Exposure to any TSSC component was associated with a lower rate of unhealthy food consumption. Compared with the unexposed intervention group, the exposed intervention for the newsletter had a higher rate of healthy eating (adjusted rate ratio = 1.18; P < .01). Compared with the unexposed intervention, the exposed intervention for the community health worker discussion had a smaller hip circumference (adjusted mean difference = -5.77 cm; P < .05) and a smaller waist circumference (adjusted mean difference = -5.25 cm; P < .05). This study provides evidence for the use of community-wide campaigns for nutrition and obesity-related outcomes in Hispanic communities. Copyright © 2017 Society for Nutrition Education and Behavior. Published by Elsevier Inc. All rights reserved.

  10. Localized calcineurin confers Ca2+-dependent inactivation on neuronal L-type Ca2+ channels.

    Science.gov (United States)

    Oliveria, Seth F; Dittmer, Philip J; Youn, Dong-ho; Dell'Acqua, Mark L; Sather, William A

    2012-10-31

    Excitation-driven entry of Ca(2+) through L-type voltage-gated Ca(2+) channels controls gene expression in neurons and a variety of fundamental activities in other kinds of excitable cells. The probability of opening of Ca(V)1.2 L-type channels is subject to pronounced enhancement by cAMP-dependent protein kinase (PKA), which is scaffolded to Ca(V)1.2 channels by A-kinase anchoring proteins (AKAPs). Ca(V)1.2 channels also undergo negative autoregulation via Ca(2+)-dependent inactivation (CDI), which strongly limits Ca(2+) entry. An abundance of evidence indicates that CDI relies upon binding of Ca(2+)/calmodulin (CaM) to an isoleucine-glutamine motif in the carboxy tail of Ca(V)1.2 L-type channels, a molecular mechanism seemingly unrelated to phosphorylation-mediated channel enhancement. But our work reveals, in cultured hippocampal neurons and a heterologous expression system, that the Ca(2+)/CaM-activated phosphatase calcineurin (CaN) is scaffolded to Ca(V)1.2 channels by the neuronal anchoring protein AKAP79/150, and that overexpression of an AKAP79/150 mutant incapable of binding CaN (ΔPIX; CaN-binding PXIXIT motif deleted) impedes CDI. Interventions that suppress CaN activity-mutation in its catalytic site, antagonism with cyclosporine A or FK506, or intracellular perfusion with a peptide mimicking the sequence of the phosphatase's autoinhibitory domain-interfere with normal CDI. In cultured hippocampal neurons from a ΔPIX knock-in mouse, CDI is absent. Results of experiments with the adenylyl cyclase stimulator forskolin and with the PKA inhibitor PKI suggest that Ca(2+)/CaM-activated CaN promotes CDI by reversing channel enhancement effectuated by kinases such as PKA. Hence, our investigation of AKAP79/150-anchored CaN reconciles the CaM-based model of CDI with an earlier, seemingly contradictory model based on dephosphorylation signaling.

  11. Isolation and characterization of higher metallofullerenes Ca@C92 and Ca@C94

    Indian Academy of Sciences (India)

    Yuliang Che; Hua Yang; Hongxiao Jin; Chunxin Lu; Ziyang Liu

    2009-05-01

    We report for the first time isolation of Ca@C92 and Ca@C94 by multi-stage highperformance liquid chromatography technique without recycling equipment. It is notable that higher metallofullerenes containing alkaline earth metal ions could be isolated although their relative yields are extremely low. Ca@C92 and Ca@C94 are also confirmed by LD-TOF mass spectrometry and characterrized by UV-Vis-NIR absorption measurements.

  12. Ca(V)1 and Ca(V)2 channels engage distinct modes of Ca(2+) signaling to control CREB-dependent gene expression.

    Science.gov (United States)

    Wheeler, Damian G; Groth, Rachel D; Ma, Huan; Barrett, Curtis F; Owen, Scott F; Safa, Parsa; Tsien, Richard W

    2012-05-25

    Activity-dependent gene expression triggered by Ca(2+) entry into neurons is critical for learning and memory, but whether specific sources of Ca(2+) act distinctly or merely supply Ca(2+) to a common pool remains uncertain. Here, we report that both signaling modes coexist and pertain to Ca(V)1 and Ca(V)2 channels, respectively, coupling membrane depolarization to CREB phosphorylation and gene expression. Ca(V)1 channels are advantaged in their voltage-dependent gating and use nanodomain Ca(2+) to drive local CaMKII aggregation and trigger communication with the nucleus. In contrast, Ca(V)2 channels must elevate [Ca(2+)](i) microns away and promote CaMKII aggregation at Ca(V)1 channels. Consequently, Ca(V)2 channels are ~10-fold less effective in signaling to the nucleus than are Ca(V)1 channels for the same bulk [Ca(2+)](i) increase. Furthermore, Ca(V)2-mediated Ca(2+) rises are preferentially curbed by uptake into the endoplasmic reticulum and mitochondria. This source-biased buffering limits the spatial spread of Ca(2+), further attenuating Ca(V)2-mediated gene expression.

  13. Talking about the scene of diet and cooking based on Pao Chu Tu(kitchen picture) of Han dynasty%从汉画像石庖厨图谈汉代饮食烹饪状况

    Institute of Scientific and Technical Information of China (English)

    杨金萍

    2009-01-01

    汉画像石庖厨图以生动写真的画面,再现了汉代饮食烹饪状况.山东诸城等地庖厨图,描绘了占代庞大忙碌的烹饪场面,包括宰杀、汲水、蒸煮、炙烤、酿酒等各种厨事活动,展示了汉代先进烹饪厨具如釜、甑、鼎、烤炉等,以及汉人繁复的烹饪技术如蒸、煮、炮、炙等,反映了汉代丰富的食物种类.汉画像石庖厨图为我们研究汉代饮食生活提供了珍贵的资料.%The Pao Chu Tu(kitchen picture)of Han dynasty stone relief reconstructed the state of dietary cooking of Han dynasty with vivid and authentic picture.The Pao Chu Tu(kitchen picture)of Shandong Zhucheng city depicted ancient enormous and busy cooking scene which involved all kinds of cooking activities such as butcher,bailing,stewing,roast and brewing wine etc,displaying the advanced kitchen ware e.g.kettle,caldron,tripod,oven and complex cooking technology of Han Chinese people such as steaming,boiling,stirring baking and roast erc,reflecting rich dietary categories of Han dynasty.The Pao Chu Tu (kitchen picture)of Han dynasty stone relief provide us precious datas for the purpose of the study of dictary living of Han dynasty.

  14. Ca(2+) signalling in the Golgi apparatus.

    Science.gov (United States)

    Pizzo, Paola; Lissandron, Valentina; Capitanio, Paola; Pozzan, Tullio

    2011-08-01

    The Golgi apparatus plays a central role in lipid and protein post-translational modification and sorting. Morphologically the organelle is heterogeneous and it is possible to distinguish stacks of flat cysternae (cis- and medial Golgi), tubular-reticular networks and vesicles (trans-Golgi). These morphological differences parallel a distinct functionality with a selective distribution and complementary roles of the enzymes found in the different compartments. The Golgi apparatus has been also shown to be involved in Ca(2+) signalling: it is indeed endowed with Ca(2+) pumps, Ca(2+) release channels and Ca(2+) binding proteins and is thought to participate in determining the spatio-temporal complexity of the Ca(2+) signal within the cell, though this role is still poorly understood. Recently, it has been demonstrated that the organelle is heterogeneous in terms of Ca(2+) handling and selective reduction of Ca(2+) concentration, both in vitro and in a genetic human disease, within one of its sub-compartment results in alterations of protein trafficking within the secretory pathway and of the entire Golgi morphology. In this paper we review the available information on the Ca(2+) toolkit within the Golgi, its heterogeneous distribution in the organelle sub-compartments and discuss the implications of these characteristics for the physiopathology of the Golgi apparatus.

  15. LATS refining ladle slag modifying with CaO-CaF2

    Institute of Scientific and Technical Information of China (English)

    Hongming Wang; Guirong Li; Zhongming Ren; Bo Li; Xuejun Zhang; Guomin Shi

    2007-01-01

    To reduce the slag sticking onto the snorkel of the ladle during the ladle alloying treatment station (LATS) process, CaO-CaF2 (the mass ratio of CaO/CaF2 is 1:1) was employed as the modifier of the LATS refining ladle slag. The effect of CaO-CaF2 on the melting point, viscosity, and desulfurizing capability of the ladle slag was investigated. The melting point of the unmodified ladle slag is 1439°C. When adding 20wt% CaO-CaF2, the melting point is decreased to 1327°C. At 1500°C, the viscosity of the unmodified ladle slag is 6.5 Pa·s, which can be decreased lower than 2 Pa·s by adding more than 10wt% CaO-CaF2. The experimental results of desulfurization of the melts show that the desulfurizing power of the ladle slag can be enhanced by adding CaO-CaF2.

  16. By Regulating Mitochondrial Ca2+-Uptake UCP2 Modulates Intracellular Ca2+.

    Directory of Open Access Journals (Sweden)

    Lukas Jaroslaw Motloch

    Full Text Available The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake via the mitochondrial calcium uniporter (MCU is highly controversial.Thus, we analyzed mCa2+-uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca2+-transients from mitochondrial ((Ca2+m and intracellular compartment ((Ca2+c in the whole-cell configuration in cardiomyocytes of wild-type (WT and UCP2-/- mice.Isolated mitochondria showed a Ru360 sensitive mCa2+-uptake, which was significantly decreased in UCP2-/- (229.4±30.8 FU vs. 146.3±23.4 FU, P0.05 and transsarcolemmal Ca2+-influx was inhibited suggesting a possible compensatory mechanism. Additionally, we observed an inhibitory effect of ATP on mCa2+-uptake in WT mitoplasts and (Ca2+m of cardiomyocytes leading to an increase of (Ca2+c while no ATP dependent effect was observed in UCP2-/-.Our results indicate regulatory effects of UCP2 on mCa2+-uptake. Furthermore, we propose, that previously described inhibitory effects on MCU by ATP may be mediated via UCP2 resulting in changes of excitation contraction coupling.

  17. TU-E-201-03: Eye Lens Dosimetry in Radiotherapy Using Contact Lens-Shaped Applicator

    Energy Technology Data Exchange (ETDEWEB)

    Park, J. [Seoul National University Hospital (Korea, Republic of)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  18. TU-E-201-01: Methods for Eye Lens Dosimetry and Studies On Lens Opacities with Interventionists

    Energy Technology Data Exchange (ETDEWEB)

    Rehani, M. [Massachusetts General Hospital (United States)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  19. « El rastro de tu sangre en la nieve » : ¿ cuento de hadas sombrío o canción kitsch ?

    OpenAIRE

    Maryse Renaud

    2012-01-01

    Este artículo propone una lectura del cuento « El rastro de tu sangre en la nieve » de Gabriel García Márquez (Doce cuentos peregrinos, 1992), observando las relaciones que el mismo establece con distintas formas de la cultura popular. Considerando el contexto del llamado « realismo mágico », donde evoluciona habitualmente la producción de García Márquez, se estudian, en este relato, las múltiples relaciones inter-textuales con distintos tópicos populares, desde el cuento de hadas o de horror...

  20. Ogļhidrātu satura izvērtējums etiķskābes baktēriju fermentēšanas kultūršķidrumā

    OpenAIRE

    Beikmanis, Nils

    2015-01-01

    Darba teorētiskajā daļā tika apskatīta literatūra par iespējamo ogļhidrātu saturu fermentēšanas kultūršķidrumā un tā analīzes metodes. Praktiskajā daļā tika noteikts kvantitatīvs un kvalitatīvs kultūršķidrumu sastāvs izmantojot Lufa-Šorla metodi un augstefektīvo šķidrumu hromatogrāfiju.

  1. Ca-Dependent Folding of Human Calumenin

    Science.gov (United States)

    Mazzorana, Marco; Hussain, Rohanah; Sorensen, Thomas

    2016-01-01

    Human calumenin (hCALU) is a six EF-hand protein belonging to the CREC family. As other members of the family, it is localized in the secretory pathway and regulates the activity of SERCA2a and of the ryanodine receptor in the endoplasmic reticulum (ER). We have studied the effects of Ca2+ binding to the protein and found it to attain a more compact structure upon ion binding. Circular Dichroism (CD) measurements suggest a major rearrangement of the protein secondary structure, which reversibly switches from disordered at low Ca2+ concentrations to predominantly alpha-helical when Ca2+ is added. SAXS experiments confirm the transition from an unfolded to a compact structure, which matches the structural prediction of a trilobal fold. Overall our experiments suggest that calumenin is a Ca2+ sensor, which folds into a compact structure, capable of interacting with its molecular partners, when Ca2+ concentration within the ER reaches the millimolar range. PMID:26991433

  2. Levels of CEA, CA153, CA199, CA724 and AFP in nipple discharge of breast cancer patients.

    Science.gov (United States)

    Zhao, Song; Mei, Yu; Wang, Yongmei; Zhu, Jiang; Zheng, Guixi; Ma, Rong

    2015-01-01

    The distinction between breast cancer and benign breast diseases with nipple discharge remains an important diagnostic challenge. The purpose of this study was to predict the potential usefulness of tumor markers in nipple discharge and to investigate the relationship of tumor markers and clinical characteristics with breast cancer.One hundred and eleven patients with nipple discharge received breast surgery from November 2013 to December 2014 were included in the study. We evaluated levels of five tumor markers (CEA, CA153, CA199, CA724 and AFP) prior to treatment. Patients were divided into two groups according to postoperative pathological results: 30 cases in breast cancer group and 81 cases in benign group. The relationships of clinical characteristics with breast cancer were investigated by multivariate analysis with a logistic regression model.It showed significant differences in levels of nipple discharge CEA (P AFP (P = 0.834) among two groups. Logistic regression analysis demonstrated complaint, age, menopause, abnormal palpable mass, CEA and CA153 were associated with breast cancer. In summary, measurements of CA199, CA724 and AFP in nipple discharge are not of great clinical value. Detecting CEA and CA153 in nipple dischargecould potentially be used for the early detection of breast cancer with in high-risk populations.

  3. SR-targeted CaMKII inhibition improves SR Ca2+ handling, but accelerates cardiac remodeling in mice overexpressing CaMKIIδC

    OpenAIRE

    Huke, Sabine; DeSantiago, Jaime; Kaetzel, Marcia A.; Mishra, Shikha; Brown, Joan H.; Dedman, John R.; Bers, Donald M.

    2010-01-01

    Cardiac myocyte overexpression of CaMKIIδC leads to cardiac hypertrophy and heart failure (HF) possibly caused by altered myocyte Ca2+ handling. A central defect might be the marked CaMKII-induced increase in diastolic sarcoplasmic reticulum (SR) Ca2+ leak which decreases SR Ca2+ load and Ca2+ transient amplitude. We hypothesized that inhibition of CaMKII near the SR membrane would decrease the leak, improve Ca2+ handling and prevent the development of contractile dysfunction and HF. To test ...

  4. Results of measuring internal Ca absorption and Ca kinetics with /sup 47/Ca in patients suffering from chronically recurrent urolithiasis

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, L.; Esther, G.; Serfling, D.; Bast, R. (Rostock Univ. (German Democratic Republic). Radiologische Klinik)

    1983-07-01

    47 patients with chronically recurrent calcium lithiasis (with between 3 and over 100 stone episodes) and an average age of 43.2 years have been examined. The Ca absorption lay between 42.7% and 90.0% of the dose, the average was 59.3% with a standard deviation of +- 12.9. This is higher than in persons with healthy kidneys. The Ca absorption is significantly lower in patients with renal insufficiency. Studies on Ca kinetics revealed turnover rates and pool sizes within the normal range.

  5. Isospin effects in {sup 40,48}Ca+{sup 40,48}Ca collisions

    Energy Technology Data Exchange (ETDEWEB)

    Henzl, V., E-mail: henzl@mit.ed [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Henzlova, D.; Kilburn, M. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Verde, G. [INFN, Sezione di Catania. 64 Via S. Sofia, I-95123, Catania (Italy); Brown, D. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Chbihi, A. [GANIL, CEA et IN2P3-CNRS, B.P. 5027, F-14076 Caen Cedex (France); Coupland, D. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Elson, J. [Departments of Chemistry and Physics, Washington University, St. Louis, MO 63130 (United States); Famiano, M. [Western Michigan University, 2229 Everett Tower, Kalamazoo, MI 49008-5252 (United States); Herlitzius, C. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Hudan, S. [Indiana Univ. Cyclotron Facility, 2401 Milo B. Sampson Lane, Bloomington, IN 47405-4001 (United States); Lee, J.; Lukyanov, S.; Lynch, W.; Rogers, A.; Sanetullaev, A. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Sobotka, L. [Departments of Chemistry and Physics, Washington University, St. Louis, MO 63130 (United States); Souza, R.T. de [Indiana Univ. Cyclotron Facility, 2401 Milo B. Sampson Lane, Bloomington, IN 47405-4001 (United States); Sun, Z.Y.; Tsang, B. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States)

    2010-03-01

    The isospin dependence of two proton correlations is studied in {sup 40}Ca+{sup 40}Ca and {sup 48}Ca+{sup 48}Ca collisions at E/A=80MeV. Measurements were performed with the HiRA detector array complemented by the 4pi Ball at NSCL. We observe a strong isospin dependence of the pp-correlation functions; however the emitting source radius extracted using the imaging technique yields no sensitivity to the isospin of the reaction system. We interpret this result as a consequence of smaller fraction of fast proton emission in the neutron rich {sup 48}Ca system.

  6. Toosendanin increases free-Ca2+ concentration in NG108-15 cells via L-type Ca2+ channels

    Institute of Scientific and Technical Information of China (English)

    Tong-hui XU; Jun DING; Yu-liang SHI

    2004-01-01

    AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+]i) in neuroblastomaxglioma hybrid cells (NG108-15 cells). METHODS: The [Ca2+]i was determined by laser-scanning confocal microscopic imaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting [Ca2+]i and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+]i was dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared after washing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+]i in differentiated NG108-15 cells. The [Ca2+]i enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+ channels.

  7. A comparison of fluorescent Ca2+ indicators for imaging local Ca2+ signals in cultured cells

    Science.gov (United States)

    Lock, Jeffrey T.; Parker, Ian

    2015-01-01

    Localized subcellular changes in Ca2+ serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca2+ signaling have been greatly facilitated by the availability of fluorescent Ca2+ indicators. The respective merits of different indicators to monitor bulk changes in cellular Ca2+ levels have been widely evaluated, but a comprehensive comparison for their use in detecting and analyzing local, subcellular Ca2+ signals is lacking. Here, we evaluated several fluorescent Ca2+ indicators in the context of local Ca2+ signals (puffs) evoked by inositol 1,4,5-trisphosphate (IP3) in cultured human neuroblastoma SH-SY5Y cells, using high-speed video-microscopy. Altogether, nine synthetic Ca2+ dyes (Fluo-4, Fluo-8, Fluo-8 high affinity, Fluo-8 low affinity, Oregon Green BAPTA-1, Cal-520, Rhod-4, Asante Calcium Red, and X-Rhod-1) and three genetically-encoded Ca2+-indicators (GCaMP6-slow, -medium and -fast variants) were tested; criteria include the magnitude, kinetics, signal-to-noise ratio and detection efficiency of local Ca2+ puffs. Among these, we conclude that Cal-520 is the optimal indicator for detecting and faithfully tracking local events; that Rhod-4 is the red-emitting indicator of choice; and that none of the GCaMP6 variants are well suited for imaging subcellular Ca2+ signals. PMID:26572560

  8. Behavior of CaO and Calcium in pure Magnesium

    Institute of Scientific and Technical Information of China (English)

    HA Seong-Ho; LEE Jin-Kyu; JO Hyung-Ho; JUNG Seung-Boo; KIM Shae K.

    2006-01-01

    Mg alloys exhibit a number of good properties such as low density, good castability and high specific strength.However, molten Mg and Mg alloys are ignited without the melt protective gases during melting and casting process due to their high reactivity.The purpose of this study is to investigate effects of Ca and CaO on pure Mg through microstructure observation, ignition test and phase analysis.With increasing Ca and CaO contents, the ignition resistance of Ca or CaO added pure Mg is increased and the grains are refined.As results of XRD and EDS, CaO is reduced to Ca in CaO added pure Mg.Mg2Ca phase is formed even in 0.1 wt.%CaO added pure Mg by reduction mechanism, while Mg2Ca phase is formed over 1.35 wt.% Ca added pure Mg.

  9. Ca2+ sparks and Ca2+ glows in superior cervical ganglion neurons

    Institute of Scientific and Technical Information of China (English)

    Li-jun YAO; Cai-hong WU; Jie LIU; Zhuan ZHOU; He-ping CHENG; Gang WANG; Kun-fu OU-YANG; Chao-liang WEI; Xian-hua WANG; Shi-rong WANG; Wei YAO; Hong-ping HUANG; Jian-hong LUO

    2006-01-01

    Aim: Ca2+ release from the endoplasmic reticulum (ER) is an integral component of neuronal Ca2+ signaling. The present study is to investigate properties of local Ca2+ release events in superior cervical ganglion (SCO) neurons. Methods: Primary cultured SCO neurons were prepared from neonatal rats (P3-P7). Low concentration of caffeine was used to induce Ca2+ release from the ER Ca2+ store, and intracellular Ca2+ was recorded by high-resolution line scan confocal imaging and the Ca2+ indicator Fluo-4. Results: Two populations of local Ca2+ release events with distinct temporal characteristics were evoked by 1.5 mmol/L caffeine near the surface membrane in the soma and the neurites of SCG neurons. Brief events similar to classic Ca2+ sparks lasted a few hundreds of milliseconds, whereas long-lasting events displayed duration up to tens of seconds. Typical somatic and neurite sparks were of 0.3- and 0.52-fold increase in local Fluo-4 fluorescence, respectively. Typical Ca2+ glows were brighter (△F/F0 approximately 0.6), but were highly confined in space. The half maximum of full duration of neurite sparks was much longer than those in the soma (685 vs 381 ms). Conclusion: Co-existence of Ca2+ sparks and Ca2+ glows in SCG neurons indicates distinctive local regulation of Ca2+ release kinetics. The local Ca2+ signals of variable, site-specific temporal length may bear important implications in encoding a "memory" of the trigger signal.

  10. Serum level of TSGF,CA242 and CA19-9 in pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    Jing-Ting Jiang; Chang-Ping Wu; Hai-Feng Deng; Ming-Yang Lu; Jun Wu; Hong-Yu Zhang; Wen-Hui Sun; Mei Ji

    2004-01-01

    AIM: To establish a method to detect the expression of the tumor specific growth factor TSGF, CA242 and CA19-9 in serum and evaluate their value in diagnosis of pancreatic cancer.METHODS: ELISA and Biochemical colorimetric assay were used to detect the serum content of TSGF, CA242 and CA19-9 in 200 normal cases, 52 pancreatitis patients and 96 pancreatic cancer patients.RESULTS: The positive likelihood ratios of TSGF, CA242and CA19-9 were 5.4, 12.6 and 6.3, respectively, and their negative likelihood ratios were 0.10, 0.19 and 0.17,respectively. With single tumor marker diagnosed pancreatic cancer, the highest sensitivity and specificity of TSGF were 91.6% and 93.5%. In combined test with 3 markers, when all of them were positive, the sensitivity changed to 77.0%and the specificity and the positive predictive value were 100%. The levels of TSGF and CA242 were significantly higher in the patients with pancreatic cancer of head than those in the patients with pancreatic cancer of body, tail and whole pancreas, but the expression of CA19-9 had no correlation with the positions of the pancreatic cancer. The sensitivity of TSGF, CA242 and CA19-9 was increased with the progress in stages of pancreatic cancer. In stage I, the sensitivity of TSGF was markedly higher than CA242 and CA19-9.CONCLUSION: The combined use of TSGF, CA242 and CA19-9 expressions can elevate the specificity for pancreatic cancer diagnosis. And it shows that it plays an important role to differentiate positions and tissue typing. It is a forepart diagnosis for the pancreatic cancer by combination checking.There is very important correlation between the three markers and the pancreatic cancer.

  11. Professional Development of College and University English Teachers in Chang-Ji-Tu Area%长吉图区域高校外语教师专业发展研究

    Institute of Scientific and Technical Information of China (English)

    徐莹

    2014-01-01

    调查长吉图区域高校外语教师专业发展状况,对调查结果进行分析,并提出外语教师专业发展途径,以期为外语教师的专业发展提供有益的借鉴,从而提高外语教师素质,提升高校教学质量,发挥高校的优势,促进长吉图开发开放先导区建设。%This study investigates foreign language teachers' professional development in Chang-Ji-Tu area, ana-lyses the results objectively. Based on the results, constructive suggestions are made on how to enhance English teachers' professional development, aiming to improve foreign language teachers' qualities and teaching quality of colleges and universities, promote the economic construction of Chang-Ji-Tu area.

  12. Identities ofJiao, Tu and Cao from Shuihudi Bamboo Slips%从睡虎地秦简看“校、徒、操”的身份

    Institute of Scientific and Technical Information of China (English)

    仝卫敏

    2012-01-01

    Jiao [校] ,tu [徒] and cao [操] recorded in "Jing Nei Pian" [境内篇] in ShangJun Shu [商君书] are associated with the ranking system advocated by Shang Yang and their identities are controversial among scholars. By referring to Qin dynasty bamboo slips discovered at Shuihudi, Hubei province, this article points out thatjiao, tu and cao belong to a lower class in the army; instead of being handymen or sappers, they are minor officials in the army descending in rank and are not part of Shang Yang' s ranking system.%“校、徒、操”见于《商君书·境内篇》,关涉商鞅爵制等级问题,学界对其身份争议较大。本文结合睡虎地秦墓竹简的相关材料,认为“校、徒、操”尽管在军中地位较低,但并非军中杂役、苦力或工程兵,而是由高到低排列的军中小吏,且并不在商鞅爵制等级范围之内。

  13. Marijas Montesori pedagoģisko principu izmantošana sajūtu attīstībai trīs līdz četrus gadus veciem bērniem

    OpenAIRE

    Dzērve, Vita

    2012-01-01

    Tēma: Marijas Montesori pedagoģisko principu izmantošana sajūtu attīstībai trīs līdz četrus gadus veciem bērniem Kvalifikācijas darba autore: Vita Dzērve Kvalifikācijas darba vadītāja: lekt.,mag. paed., I. Šūmane Kvalifikācijas darba apjoms: 57 lpp.,16 attēli, 1 pielikums, 19 izmantoti literatūras avoti Pētījuma mērķis: izpētīt un praksē aprobēt M. Montesori pedagoģiskos principus trīs līdz četrus gadus veciem bērniem sajūtu attīstības veicināšanai. Darba pirmajā daļā ...

  14. Study on Roasting Decomposition of Mixed Rare Earth Concentrate in CaO-NaCl-CaCl2

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The decomposed process of bastnaesite, monazite and mixed rare earth concentrate in CaO-CaCl-CaCl2 was studied by means of TG-DTA method. The relationship among decomposition ratio, roasting temperature, and CaO-NaCl addition was studied by the quadratic regression orthogonal analysis, and then the regression equation was obtained. Through analysis, the optimum process conditions of mixed rare earth concentrate decomposed by CaO-CaCl-CaCl2 were obtained as follows: roasting temperature: 700 ℃, CaO addition: 15%, NaCl-CaCl2 addition: 10%, roasting time: 60 min, the decomposition ratio: 91.3%.

  15. Aging and CaMKII Alter Intracellular Ca2+ Transients and Heart Rhythm in Drosophila melanogaster

    Science.gov (United States)

    Santalla, Manuela; Valverde, Carlos A.; Harnichar, Ezequiel; Lacunza, Ezequiel; Aguilar-Fuentes, Javier; Mattiazzi, Alicia; Ferrero, Paola

    2014-01-01

    Aging is associated to disrupted contractility and rhythmicity, among other cardiovascular alterations. Drosophila melanogaster shows a pattern of aging similar to human beings and recapitulates the arrhythmogenic conditions found in the human heart. Moreover, the kinase CaMKII has been characterized as an important regulator of heart function and an arrhythmogenic molecule that participate in Ca2+ handling. Using a genetically engineered expressed Ca2+ indicator, we report changes in cardiac Ca2+ handling at two different ages. Aging prolonged relaxation, reduced spontaneous heart rate (HR) and increased the occurrence of arrhythmias, ectopic beats and asystoles. Alignment between Drosophila melanogaster and human CaMKII showed a high degree of conservation and indicates that relevant phosphorylation sites in humans are also present in the fruit fly. Inhibition of CaMKII by KN-93 (CaMKII-specific inhibitor), reduced HR without significant changes in other parameters. By contrast, overexpression of CaMKII increased HR and reduced arrhythmias. Moreover, it increased fluorescence amplitude, maximal rate of rise of fluorescence and reduced time to peak fluorescence. These results suggest that CaMKII in Drosophila melanogaster acts directly on heart function and that increasing CaMKII expression levels could be beneficial to improve contractility. PMID:25003749

  16. Prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma

    Science.gov (United States)

    Wang, Wei; Chen, Xiao-Long; Zhao, Shen-Yu; Xu, Yu-Hui; Zhang, Wei-Han; Liu, Kai; Chen, Xin-Zu; Yang, Kun; Zhang, Bo; Chen, Zhi-Xin; Chen, Jia-Ping; Zhou, Zong-Guang; Hu, Jian-Kun

    2016-01-01

    The prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma (GC) has been widely reported and is still under debate. Here, we evaluated the prognostic significance of preoperative serum CA125, CA19-9 and CEA in patients with GC. 1692 patients with GC who underwent gastrectomy were divided into the training (from January 2005 to December 2011, n = 1024) and the validation (from January 2012 to December 2013, n = 668) cohorts. Positive groups of CA125 (> 13.72 U/ml), CA19-9 (> 23.36 U/ml) and CEA (> 4.28 ng/ml) were significantly associated with more advanced clinicopathological traits and worse outcomes than that of negative groups (all P < 0.01). In Cox regression analysis, tumor size (P < 0.001, P = 0.005), pTNM stage (P < 0.001, P < 0.001) and CA125 (P = 0.026, P = 0.005) were independent prognostic factors both in two cohorts. Nomograms of these two cohorts based on the number of positive serum tumor markers (NPTM) were more accurate in prognostic prediction than TNM stage alone. Our findings suggested that elevated preoperative serum CA125, CA19-9 and CEA were associated with more advanced clinicopathological traits and less favorable outcomes. In addition, CA125 as an independent prognostic factor should be further investigated. Nomogram based on NPTM could accurately predict the prognosis of GC patients. PMID:27097114

  17. Regulated release of Ca2+ from respiring mitochondria by Ca2+/2H+ antiport.

    Science.gov (United States)

    Fiskum, G; Lehninger, A L

    1979-07-25

    Simultaneous measurements of oxygen consumption and transmembrane transport of Ca2+, H+, and phosphate show that the efflux of Ca2+ from respiring tightly coupled rat liver mitochondria takes place by an electroneutral Ca2+/2H+ antiport process that is ruthenium red-insensitive and that is regulated by the oxidation-reduction state of the mitochondrial pyridine nucleotides. When mitochondrial pyridine nucleotides are kept in a reduced steady state, the efflux of Ca2+ is inhibited; when they are in an oxidized state, Ca2+ efflux is activated. These processes were demonstrated by allowing phosphate-depleted mitochondria respiring on succinate in the presence of rotenone to take up Ca2+ from the medium. Upon subsequent addition of ruthenium red to block Ca2+ transport via the electrophoretic influx pathway, and acetoacetate, to bring mitochondrial pyridine nucleotides into the oxidized state, Ca2+ efflux and H+ influx ensued. The observed H+ influx/Ca2+ efflux ratio was close to the value 2.0 predicted for the operation of an electrically neutral Ca2+/2H+ antiport process.

  18. Ischemic damage in hippocampal CA1 is dependent on glutamate release and intact innervation from CA3

    DEFF Research Database (Denmark)

    Benveniste, H; Jørgensen, M B; Sandberg, M

    1989-01-01

    The removal of glutamatergic afferents to CA1 by destruction of the CA3 region is known to protect CA1 pyramidal cells against 10 min of transient global ischemia. To investigate further the pathogenetic significance of glutamate, we measured the release of glutamate in intact and CA3-lesioned CA...

  19. EnviroAtlas - Fresno, CA - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the Fresno, CA EnviroAtlas area. The block groups are from the US Census Bureau and are included/excluded based on...

  20. Role of Ca++ in Shoot Gravitropism. [avena

    Science.gov (United States)

    Rayle, D. L.

    1985-01-01

    A cornerstone in the argument that Ca(2+) levels may regulate growth is the finding the EGTA promotes straight growth. The usual explanation for these results is that Ca(2+) chelation from cell walls results in wall loosening and thus accelerated straight growth. The ability of frozen-thawed Avena coleoptile tissue (subjected to 15g tension) to extend in response to EGTA and Quin II was examined. The EGTA when applied in weakly buffered (i.e., 0.1mM) neutral solutions initiates rapid extension. When the buffer strength is increased, similar concentrations of EGTA produce no growth response. This implies when EGTA liberated protons are released upon Ca(2+) chelation they can either initiate acid growth (low buffer conditions) or if consumed (high buffer conditions) have no effect. Thus Ca(2+) chelation in itself apparently does not result in straight growth.

  1. CaMath user`s guide

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Ben-chin; Daly, B.

    1994-07-13

    CaMath is an external Mathematica package which can be loaded into Mathematica by a user. CaMath consists of a special set of channel access functions which provides the Mathematica users with easy and flexible access of channel information across the IOC networks. It also provides a complete set of process variable event monitoring functions. The available functions for CaMath, their functionality, and their syntax are described herein. This document also gives examples how a Mathematica user can interface to channel access devices. It is assumed that the user is already familiar with using Mathematica. Few examples of Mathematica module of using CaMath functions are also given in this document.

  2. CA Investment Casting Process of Complex Castings

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    CA (Computer aided) investment casting technique used in superalloy castings of aerospace engine parts was presented. CA investment casting integrated computer application, RP (Rapid Prototyping) process, solidification simulation and investment casting process. It broke the bottle neck of making metal die. Solid model of complex parts were produced by UGII or other software, then translated into STL(Stereolithography) file, after RP process of SLS(Selective Laser Sintering), wax pattern used in investment ...

  3. YouthCaN 2001 / Sirje Janikson

    Index Scriptorium Estoniae

    Janikson, Sirje

    2001-01-01

    Aprillis 2001 toimus keskkonnateemaline õpilaskonverents YouthCaN 2001 Ameerika Loodusajaloo Muuseumis New Yorkìs. 35 seminarist ühe viis läbi Tartu Noorte Loodusmaja geoloogia ja keskkonnaringi esindus, tutvustati loodusmaja keskkonnaprojekte ja räägiti keskkonnaalaste veebilehtede koostamise kogemustest. YouthCaN (Youth Communicating and Networking) on rahvusvaheline noorte organisatsioon, mis vahendab kogemusi ja uusi ideid elukeskkonnast huvitatud noorte hulgas

  4. YouthCaN 2001 / Sirje Janikson

    Index Scriptorium Estoniae

    Janikson, Sirje

    2001-01-01

    Aprillis 2001 toimus keskkonnateemaline õpilaskonverents YouthCaN 2001 Ameerika Loodusajaloo Muuseumis New Yorkìs. 35 seminarist ühe viis läbi Tartu Noorte Loodusmaja geoloogia ja keskkonnaringi esindus, tutvustati loodusmaja keskkonnaprojekte ja räägiti keskkonnaalaste veebilehtede koostamise kogemustest. YouthCaN (Youth Communicating and Networking) on rahvusvaheline noorte organisatsioon, mis vahendab kogemusi ja uusi ideid elukeskkonnast huvitatud noorte hulgas

  5. Design and testing of Ground Penetrating Radar equipment dedicated for civil engineering applications: ongoing activities in Working Group 1 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Manacorda, Guido; Persico, Raffaele

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 1 'Novel GPR instrumentation' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Working Group 1 (WG1) of the Action focuses on the development of innovative GPR equipment dedicated for civil engineering applications. It includes three Projects. Project 1.1 is focused on the 'Design, realisation and optimisation of innovative GPR equipment for the monitoring of critical transport infrastructures and buildings, and for the sensing of underground utilities and voids.' Project 1.2 is concerned with the 'Development and definition of advanced testing, calibration and stability procedures and protocols, for GPR equipment.' Project 1.3 deals with the 'Design, modelling and optimisation of GPR antennas.' During the first year of the Action, WG1 Members coordinated between themselves to address the state of the art and open problems in the scientific fields identified by the above-mentioned Projects [1, 2]. In carrying our this work, the WG1 strongly benefited from the participation of IDS Ingegneria dei Sistemi, one of the biggest GPR manufacturers, as well as from the contribution of external experts as David J. Daniels and Erica Utsi, sharing with the Action Members their wide experience on GPR technology and methodology (First General Meeting, July 2013). The synergy with WG2 and WG4 of the Action was useful for a deep understanding of the problems, merits and limits of available GPR equipment, as well as to discuss how to quantify the reliability of GPR results. An

  6. Mitochondrial Ca2+ uptake in skeletal muscle health and disease

    CERN Document Server

    Zhou, Jingsong; Yi, Jianxun

    2016-01-01

    Muscle uses Ca2+ as a messenger to control contraction and relies on ATP to maintain the intracellular Ca2+ homeostasis. Mitochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca2+ from their surroundings, a process called mitochondrial Ca2+ uptake. Under physiological conditions, Ca2+ uptake into mitochondria promotes ATP production. Excessive uptake causes mitochondrial Ca2+ overload, which activates downstream adverse responses leading to cell dysfunction. Moreover, mitochondrial Ca2+ uptake could shape spatio-temporal patterns of intracellular Ca2+ signaling. Malfunction of mitochondrial Ca2+ uptake is implicated in muscle degeneration. Unlike non-excitable cells, mitochondria in muscle cells experience dramatic changes of intracellular Ca2+ levels. Besides the sudden elevation of Ca2+ level induced by action potentials, Ca2+ transients in muscle cells can be as short as a few milliseconds during a single twitch or as long as min...

  7. PIK3CA in colorectal cancer

    Directory of Open Access Journals (Sweden)

    Gieri eCathomas

    2014-03-01

    Full Text Available PIK3CA, the catalytic subunit of PI3K, is mutated in many different tumours, including colorectal cancer. Mutations of PIK3CA have been reported in 10 – 20% of colorectal cancer, about 80% of mutations found in two hot spots in exon 9 and exon 20. In RAS wild-type colorectal cancers, PIK3CA mutations have been associated with a worse clinical outcome and with a negative prediction of a response to targeted therapy by anti-EGFR monoclonal antibodies. However, these findings have not been confirmed in all studies and subsequent more detailed analysis has revealed that these effects may be restricted to mutations in Exon 20. Finally, mutations in PIK3CA may be the long sought biomarker for successful adjuvant therapy with aspirin in patients with colorectal cancer. Therefore, PIK3CA mutations appear to be a promising predictive biomarker; however, further data are needed to conclusively define the impact of somatic mutations in the PIK3CA gene for the management of patients with colorectal cancer.

  8. X-Ray Data on Extraterrestrial CA Dialuminate (CaAl4O7)

    Science.gov (United States)

    Weber, D.; Ross, C. R., II; Bischoff, A.

    1993-07-01

    After the first discovery of Ca-dialuminate (CaAl4O7) in Allende [1], in recent years this phase has been found in several carbonaceous chondrites. Ca- dialuminate is a major phase in Ca,Al-rich inclusions from ALH85085 (e.g., [2]) and a dominating phase in CAIs from Acfer 182 ([3,4]). X-ray data on Ca-dialuminate are known from synthetic (e.g., [5-8]; cell constants) and terrestrial CaAl4O7 ([9]; only d-spacings), but are not available from extraterrestrial Ca-dialuminate. We report here the results of the first X-ray study of extraterrestrial Ca- dialuminate. The data (Table 1) were obtained by microdiffraction using a Rigaku PSPC microdiffractometer at the Bayerisches Geoinstitut. Ni-filtered Cr radiation was used with a direct beam diameter of about 50 micrometers. This powder diffraction method allows in situ measurement of polycrystalline Ca- dialuminate in a thin section. The CaAl4O7-rich inclusion 022/9 described in [4], consisting of a ~200-micrometer-sized core of Ca-dialuminate surrounded by layers of melilite and Ca-pyroxene, was chosen for analysis. The polycrystalline core contains only a small number of tiny inclusions (especially perovskite) and is therefore an excellent candidate for an X-ray study. For determination of the d-spacings of Ca-dialuminate an external standard (Ag6Ge10P12) was used for detector calibration. A large number of reflections could be indexed based upon comparison with the X-ray pattern of synthetic CaAl4O7 available in the JCPDS compilation [7]. The comparison was simplified because of the high purity of CaAl4O7 in inclusion 022/9 [4], and suggests the same structure for synthetic and extraterrestrial Ca-dialuminate. For determination of lattice parameters (cell constants, cell volume) refinement calculations were made based on 14 reflections (Table 1). The data for extraterrestrial CaAl4O7 shown in Table 1 indicate a close similarity to those obtained for synthetic CaAl4O7. The cell constants a, b, and therefore the cell

  9. Ultrastructural and immunohistochemical localization of plasma membrane Ca2+-ATPase 4 in Ca2+-transporting epithelia

    DEFF Research Database (Denmark)

    Alexander, R Todd; Beggs, Megan R; Zamani, Reza

    2015-01-01

    Plasma Membrane Ca(2+)-ATPase's (PMCA) participate in epithelial Ca(2+) transport and intracellular Ca(2+) signaling. The Pmca4 isoform is enriched in distal nephron isolates and decreased in mice lacking the epithelial Ca(2+) channel, Trpv5. We therefore hypothesized that Pmca4 plays a significant...... in distal nephron cells at both the basolateral membrane and intracellular perinuclear compartments, but not submembranous vesicles, suggesting rapid trafficking to the plasma membrane is unlikely to occur in vivo. Pmca4 expression was not altered by perturbations in Ca(2+) balance, pointing...... detected Pmca1 in lateral membranes of enterocytes. In kidney, Pmca4 showed broad localization to the distal nephron. In mouse, expression was most abundant in segments coexpressing the epithelial Ca(2+) channel, Trpv5. Significant, albeit lower expression, was also evident in the region encompassing...

  10. Sarcolemmal Ca(2+)-entry through L-type Ca(2+) channels controls the profile of Ca(2+)-activated Cl(-) current in canine ventricular myocytes.

    Science.gov (United States)

    Horváth, Balázs; Váczi, Krisztina; Hegyi, Bence; Gönczi, Mónika; Dienes, Beatrix; Kistamás, Kornél; Bányász, Tamás; Magyar, János; Baczkó, István; Varró, András; Seprényi, György; Csernoch, László; Nánási, Péter P; Szentandrássy, Norbert

    2016-08-01

    Ca(2+)-activated Cl(-) current (ICl(Ca)) mediated by TMEM16A and/or Bestrophin-3 may contribute to cardiac arrhythmias. The true profile of ICl(Ca) during an actual ventricular action potential (AP), however, is poorly understood. We aimed to study the profile of ICl(Ca) systematically under physiological conditions (normal Ca(2+) cycling and AP voltage-clamp) as well as in conditions designed to change [Ca(2+)]i. The expression of TMEM16A and/or Bestrophin-3 in canine and human left ventricular myocytes was examined. The possible spatial distribution of these proteins and their co-localization with Cav1.2 was also studied. The profile of ICl(Ca), identified as a 9-anthracene carboxylic acid-sensitive current under AP voltage-clamp conditions, contained an early fast outward and a late inward component, overlapping early and terminal repolarizations, respectively. Both components were moderately reduced by ryanodine, while fully abolished by BAPTA, but not EGTA. [Ca(2+)]i was monitored using Fura-2-AM. Setting [Ca(2+)]i to the systolic level measured in the bulk cytoplasm (1.1μM) decreased ICl(Ca), while application of Bay K8644, isoproterenol, and faster stimulation rates increased the amplitude of ICl(Ca). Ca(2+)-entry through L-type Ca(2+) channels was essential for activation of ICl(Ca). TMEM16A and Bestrophin-3 showed strong co-localization with one another and also with Cav1.2 channels, when assessed using immunolabeling and confocal microscopy in both canine myocytes and human ventricular myocardium. Activation of ICl(Ca) in canine ventricular cells requires Ca(2+)-entry through neighboring L-type Ca(2+) channels and is only augmented by SR Ca(2+)-release. Substantial activation of ICl(Ca) requires high Ca(2+) concentration in the dyadic clefts which can be effectively buffered by BAPTA, but not EGTA.

  11. Izlaganje na javni uvid podataka o nekretninama i utvrđivanje prava na zemljištu u funkciji uspostave jedinstvenog registra zemljišta i prava na zemljištu u Brčko distriktu BiH : Public presentation of data on real-estate and determination of rights in function of the establishment of a single registry of real-estate and real rights to real-estate in the Brcko district of B&H

    Directory of Open Access Journals (Sweden)

    Faruk Latifović

    2014-12-01

    Full Text Available Zakon o registru zemljišta i prava na zemljištu Brčko distrikta Bosne i Hercegovine je propisao uspostavu jedinstvene evidencije nekretnina i stvarnih prava na nekretninama u Brčko distriktu Bosne i Hercegovine. Poslovi uspostave jedinstvene evidencije, u segmentu izlaganja na javni uvid podataka o nekretninama i utvrđivanje prava, su povjereni Vijeću za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta Bosne i Hercegovine. Autor se, u ovom radu, uz nezaobilazan kraći prikaz historijata javnih evidencija na nekretninama u Bosni i Hercegovini, bavi organizacijom, nadležnostima i načinom rada Vijeća za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta BIH. Nakon što bude uspješno okončan projekat izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu u Brčko distriktu BiH, svi geodetsko – katastarski podaci o nepokretnostima kao i podaci o njihovom pravnom statusu će biti vođeni u Registru zemljišta i prava na zemljištu Osnovnog suda Brčko distrikta BiH. : The Law of Land Registry and Rights over Land of the Brčko District of B&H stipulated the establishment of a single registry of real-estate and real rights to real-estate in the Brčko District of B&H. Activities of the establishment of a single registry, in the segment of public presentation of data on real-estate and determination of rights, were entrusted to the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. In this paper, the author presents the inevitable short overview of the history of real-estate registries in Bosnia and Herzegovina and he also deals with organization, competencies and manner of work of the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. After a successful completion of the project of presentation of data on real-estate and

  12. Endothelin-1 induces intracellular [Ca2+] increase via Ca2+ influx through the L-type Ca2+ channel, Ca2+-induced Ca2+ release and a pathway involving ETA receptors, PKC, PKA and AT1 receptors in cardiomyocytes

    Institute of Scientific and Technical Information of China (English)

    ZENG QingHua; LI XingTing; ZHONG GuoGan; ZHANG WenJie; SUN ChengWen

    2009-01-01

    Using fura-2-acetoxymethyl eater (AM) fluorescence imaging and patch clamp techniques, we found that endothelin-1 (ET-1) significantly elevated the intracellular calcium level ([Ca2+]1) in a dose-dependent manner and activated the L-type Ca2+ channel in cardiomyocytes isolated from rats.The effect of ET-1 on [Ca2+]1 elevation was abolished in the presence of the ETA receptor blocker BQ123,but was not affected by the ETa receptor blocker BQ788. ET-1-induced an increase in [Ca2+]1, which was inhibited 46.7% by pretreatment with a high concentration of ryanodine (10 μmol/L), a blocker of the ryanodine receptor. The ET-1-induced [Ca2+]i increase was also inhibited by the inhibltors of protein kinase A (PKA), protein kinase C (PKC) and angiotensin type 1 receptor (AT1 receptor). We found that ET-1 induced an enhancement of the amplitude of the whole cell L-type Ca2+ channel current and an Increase of open-state probability (NPo) of an L-type single Ca2+ channel. BQ123 completely blocked the ET-1-induced increase in calcium channel open-state probability. In this study we demonstrated that ET-1 regulates calcium overload through a series of mechanisms that include L-type Ca2+ channel activation and Ca2+-induced Ca2+ release (CICR). ETa receptors, PKC, PKA and AT1 receptors may also contribute to this pathway.

  13. Endothelin-1 induces intracellular [Ca2+] increase via Ca2+ influx through the L-type Ca2+ channel, Ca2+-induced Ca2+ release and a pathway involving ETA receptors, PKC, PKA and AT1 receptors in cardiomyocytes

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Using fura-2-acetoxymethyl ester (AM) fluorescence imaging and patch clamp techniques, we found that endothelin-1 (ET-1) significantly elevated the intracellular calcium level ([Ca2+]i) in a dose-dependent manner and activated the L-type Ca2+ channel in cardiomyocytes isolated from rats. The effect of ET-1 on [Ca2+]i elevation was abolished in the presence of the ETA receptor blocker BQ123, but was not affected by the ETB receptor blocker BQ788. ET-1-induced an increase in [Ca2+]i, which was inhibited 46.7% by pretreatment with a high concentration of ryanodine (10 μmol/L), a blocker of the ryanodine receptor. The ET-1-induced [Ca2+]i increase was also inhibited by the inhibitors of protein kinase A (PKA), protein kinase C (PKC) and angiotensin type 1 receptor (AT1 receptor). We found that ET-1 induced an enhancement of the amplitude of the whole cell L-type Ca2+ channel current and an increase of open-state probability (NPo) of an L-type single Ca2+ channel. BQ123 completely blocked the ET-1-induced increase in calcium channel open-state probability. In this study we demonstrated that ET-1 regulates calcium overload through a series of mechanisms that include L-type Ca2+ channel activation and Ca2+-induced Ca2+ release (CICR). ETA receptors, PKC, PKA and AT1 receptors may also contribute to this pathway.

  14. Ca(2+-dependent regulation of the Ca(2+ concentration in the myometrium mitochondria. II. Ca(2+ effects on mitochondria membranes polarization and [Ca(2+](m

    Directory of Open Access Journals (Sweden)

    L. G. Babich

    2017-06-01

    Full Text Available It is known that Ca2+ accumulation in the mitochondria undergoes complex regulation by Ca2+ itself. But the mechanisms of such regulation are still discussed. In this paper we have shown that Ca ions directly or indirectly regulate the level of myometrium mitochondria membranes polarization. The additions of 100 µM Ca2+ were accompanied by depolarization of the mitochondria membranes. The following experiments were designed to study the impact of Ca2+ on the myometrium mitochondria [Ca2+]m. Isolated myometrium mitochondria were preincubated without or with 10 μM Са2+ followed by 100 μM Са2+ addition. Experiments were conducted in three mediums: without ATP and Mg2+ (0-medium, in the presence of 3 mM Mg2+ (Mg-medium and 3 mM Mg2+ + 3 mM ATP (Mg,ATP-medium. It was shown that the effects of 10 μM Са2+ addition were different in different mediums, namely in 0- and Mg-medium the [Ca2+]m values increased, whereas in Mg,ATP-medium statistically reliable changes were not registered. Preincubation of mitochondria with 10 μM Са2+ did not affect the [Ca2+]m value after the addition of 100 μM Са2+. The [Ca2+]m values after 100 μM Са2+ addition were the same in 0- and Mg,ATP-mediums and somewhat lower in Mg-medium. Preliminary incubation of mitochondria with 10 μM Са2+ in 0- and Mg-mediums reduced changes of Fluo 4 normalized fluorescence values that were induced by 100 μM Са2+ additions, but in Mg,ATP-medium such differences were not recorded. It is concluded that Са2+ exchange in myometrium mitochondria is regulated by the concentration of Ca ions as in the external medium, so in the matrix of mitochondria. The medium composition had a significant impact on the [Са2+]m values in the absence of exogenous cation. It is suggested that light increase of [Са2+]m before the addition of 100 μM Са2+ may have a positive effect on the functional activity of the mitochondria.

  15. Physical conditions in CaFe interstellar clouds

    CERN Document Server

    Gnacinski, P

    2007-01-01

    Interstellar clouds that exhibit strong Ca I and Fe I lines were called CaFe clouds. The ionisation equilibrium equations were used to model the column densities of Ca II, Ca I, K I, Na I, Fe I and Ti II in CaFe clouds. The chemical composition of CaFe clouds is that of the Solar System and no depletion of elements onto dust grains is seen. The CaFe clouds have high electron densities n=1 cm^-3 that leads to high column densities of neutral Ca and Fe.

  16. Physical conditions in CaFe interstellar clouds

    OpenAIRE

    Gnacinski, P.; Krogulec, M.

    2007-01-01

    Interstellar clouds that exhibit strong Ca I and Fe I lines were called CaFe clouds. The ionisation equilibrium equations were used to model the column densities of Ca II, Ca I, K I, Na I, Fe I and Ti II in CaFe clouds. The chemical composition of CaFe clouds is that of the Solar System and no depletion of elements onto dust grains is seen. The CaFe clouds have high electron densities n=1 cm^-3 that leads to high column densities of neutral Ca and Fe.

  17. Mitochondrial calcium uniporter MCU supports cytoplasmic Ca2+ oscillations, store-operated Ca2+ entry and Ca2+-dependent gene expression in response to receptor stimulation.

    Directory of Open Access Journals (Sweden)

    Krishna Samanta

    Full Text Available Ca2+ flux into mitochondria is an important regulator of cytoplasmic Ca2+ signals, energy production and cell death pathways. Ca2+ uptake can occur through the recently discovered mitochondrial uniporter channel (MCU but whether the MCU is involved in shaping Ca2+ signals and downstream responses to physiological levels of receptor stimulation is unknown. Here, we show that modest stimulation of leukotriene receptors with the pro-inflammatory signal LTC4 evokes a series of cytoplasmic Ca2+ oscillations that are rapidly and faithfully propagated into mitochondrial matrix. Knockdown of MCU or mitochondrial depolarisation, to reduce the driving force for Ca2+ entry into the matrix, prevents the mitochondrial Ca2+ rise and accelerates run down of the oscillations. The loss of cytoplasmic Ca2+ oscillations appeared to be a consequence of enhanced Ca2+-dependent inactivation of InsP3 receptors, which arose from the loss of mitochondrial Ca2+ buffering. Ca2+ dependent gene expression in response to leukotriene receptor activation was suppressed following knockdown of the MCU. In addition to buffering Ca2+ release, mitochondria also sequestrated Ca2+ entry through store-operated Ca2+ channels and this too was prevented following loss of MCU. MCU is therefore an important regulator of physiological pulses of cytoplasmic Ca2+.

  18. Mitochondrial calcium uniporter MCU supports cytoplasmic Ca2+ oscillations, store-operated Ca2+ entry and Ca2+-dependent gene expression in response to receptor stimulation.

    Science.gov (United States)

    Samanta, Krishna; Douglas, Sophie; Parekh, Anant B

    2014-01-01

    Ca2+ flux into mitochondria is an important regulator of cytoplasmic Ca2+ signals, energy production and cell death pathways. Ca2+ uptake can occur through the recently discovered mitochondrial uniporter channel (MCU) but whether the MCU is involved in shaping Ca2+ signals and downstream responses to physiological levels of receptor stimulation is unknown. Here, we show that modest stimulation of leukotriene receptors with the pro-inflammatory signal LTC4 evokes a series of cytoplasmic Ca2+ oscillations that are rapidly and faithfully propagated into mitochondrial matrix. Knockdown of MCU or mitochondrial depolarisation, to reduce the driving force for Ca2+ entry into the matrix, prevents the mitochondrial Ca2+ rise and accelerates run down of the oscillations. The loss of cytoplasmic Ca2+ oscillations appeared to be a consequence of enhanced Ca2+-dependent inactivation of InsP3 receptors, which arose from the loss of mitochondrial Ca2+ buffering. Ca2+ dependent gene expression in response to leukotriene receptor activation was suppressed following knockdown of the MCU. In addition to buffering Ca2+ release, mitochondria also sequestrated Ca2+ entry through store-operated Ca2+ channels and this too was prevented following loss of MCU. MCU is therefore an important regulator of physiological pulses of cytoplasmic Ca2+.

  19. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  20. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  1. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale

  2. Relationship Between Ca2+-CaM and Ethylene-Induced PG Activity in Tomato Fruit

    Institute of Scientific and Technical Information of China (English)

    GU Cai-qin; XI Yu-fang; GUAN Jun-feng; LI Guang-min

    2003-01-01

    Polygalacturonase (PG) was studied during ripening and senescence of postharvest tomatofruit at pink stage at low and normal temperature. The results showed that the PG activity increased, thendecreased during ripening and senescence of tomato. Low temperature inhibited but ethylene enhanced PGactivity. Ethylene also enhanced caimodulin content, which was dependent on Ca2+ concentration in cell.When EGTA(Ca2+ chelator), verapamil (Vp) and LaCl3 (Ca2+ channel blockers), trifluoperazine and chloro-promaize (two CaM antagonisms) were used to treat tomato fruit at green mature stage with ethylene, theycould reverse ethylene-induced increase in PG activity, but Vp, chloropromaize (CPZ), trifluoperazine(TFP) could not directly influence PG activity, which indirectly indicated that influx of Ca2+ from the ex-tracellular space including the cell wall via the Ca2+ channel localized in plasma membrane and CaM were re-quired for ethylene-induced PG activity increase and that ethylene signal transduction may be related to Ca2+- CaM messenger system.

  3. Negative feedback from CaSR signaling to aquaporin-2 sensitizes vasopressin to extracellular Ca2.

    Science.gov (United States)

    Ranieri, Marianna; Tamma, Grazia; Di Mise, Annarita; Russo, Annamaria; Centrone, Mariangela; Svelto, Maria; Calamita, Giuseppe; Valenti, Giovanna

    2015-07-01

    We previously described that high luminal Ca(2+) in the renal collecting duct attenuates short-term vasopressin-induced aquaporin-2 (AQP2) trafficking through activation of the Ca(2+)-sensing receptor (CaSR). Here, we evaluated AQP2 phosphorylation and permeability, in both renal HEK-293 cells and in the dissected inner medullary collecting duct, in response to specific activation of CaSR with NPS-R568. In CaSR-transfected cells, CaSR activation drastically reduced the basal levels of AQP2 phosphorylation at S256 (AQP2-pS256), thus having an opposite effect to vasopressin action. When forskolin stimulation was performed in the presence of NPS-R568, the increase in AQP2-pS256 and in the osmotic water permeability were prevented. In the freshly isolated inner mouse medullar collecting duct, stimulation with forskolin in the presence of NPS-R568 prevented the increase in AQP2-pS256 and osmotic water permeability. Our data demonstrate that the activation of CaSR in the collecting duct prevents the cAMP-dependent increase in AQP2-pS256 and water permeability, counteracting the short-term vasopressin response. By extension, our results suggest the attractive concept that CaSR expressed in distinct nephron segments exerts a negative feedback on hormones acting through cAMP, conferring high sensitivity of hormone to extracellular Ca(2+). © 2015. Published by The Company of Biologists Ltd.

  4. Heat and hyposmotic stimulation increase in [Ca2+]i by Ca2+ influx in rat synoviocytes

    Institute of Scientific and Technical Information of China (English)

    SUN WenWu; HU Fen; YANG WenXiu

    2008-01-01

    Rheumatoid arthritis (RA), which is marked by inflammatory synovitis, is a common, chronic autoimmune-disease, whose pathogenesis is complex and still unclear. In order to explore the effects of heat and hyposmotic stimuli on synoviocytes in rheumatoid arthritis, the changes of [Ca2+]i induced by heat, hyposmotic and 4α-PDD stimuli were observed in synoviocytes. [Ca2+]i elevation induced by heat ≥ 28℃, hyposmotic and 4α-PDD stimuli is found to be positively relative to increasing temperature, decreasing osmolality and rising concentration of 4α-PDD. Results show that there is reciprocity among these stimuli and desensitization, and that [Ca2+]i elevation depends on Ca2+ influx, but not necessarily links to Ca2+ release from intracellular stores and voltage-dependent Ca2+ channel in synoviocytes. The above characteristics of Ca2+ influx are similar to those of TRPV4. A probable mechanism has been suggested that heat and hyposmotic stimulation might increase the level of [Ca2+]i by activating the TRPV4-like channel and Ca2+ influx in the synoviocytes.

  5. CaCl2-CaO熔盐中金属钙的电沉积行为研究

    Institute of Scientific and Technical Information of China (English)

    郭君康

    2014-01-01

    在900℃下,采用循环伏安法对CaCl2-CaO熔盐中金属钙的电沉积行为进行了研究.研究结果表明,在CaCl2熔盐中加入CaO后,熔盐中Ca2+的还原电位正移,且Ca2+的还原过程受扩散控制.

  6. Should Degree Programs in Biomedical and Health Informatics be Dedicated or Integrated? : Reflections and Recommendations after more than 40 Years of Medical Informatics Education at TU Braunschweig, including 10 Years of B.Sc. and 15 Years of M.Sc. Integrated Degree Curricula.

    Science.gov (United States)

    Haux, Reinhold; Marschollek, Michael; Wolf, Klaus-Hendrik; Zeisberg, Ute

    2017-07-01

    Education in biomedical and health informatics (BMHI) has been established in many countries throughout the world. For degree programs in BMHI we can distinguish between those that are completely stand-alone or dedicated to the discipline vs. those that are integrated within another program. After running integrated degree medical informatics programs at TU Braunschweig for 10 years at the B.Sc. and for 15 years at the M.Sc level, we (1) report about this educational approach, (2) analyze recommendations on, implementations of, and experiences with degree educational programs in BMHI worldwide, (3) summarize our lessons learned with the integrated approach at TU Braunschweig, and (4) suggest an answer to the question, whether degree programs in biomedical and health informatics should be dedicated or integrated. According to our experience at TU Braunschweig and based on our analysis of publications, there is a clear dominance of dedicated degree programs in BMHI. The specialization in medical informatics within a computer science program, as offered at TU Braunschweig, may be a good way of implementing an integrated, informatics-based approach to medical informatics, in particular if a dual degree option can be chosen. The option of curricula leading to double degrees, i.e. in this case to two separate degrees in computer science and in medical informatics might, however, be a better solution.

  7. Functional expression of Na-Ca exchanger clones measured with the fluorescent Ca(2+)-indicating dye fluo-3.

    Science.gov (United States)

    Schnetkamp, P P

    1996-01-01

    The process of Ca2+ homeostasis is of prime importance to all cells because of the ubiquitous role of cytoplasmic Ca2+ as an intracellular messenger and the cytotoxicity of sustained elevated cytosolic Ca2+ concentrations. Two classes of plasma membrane proteins are responsible for maintaining cytosolic free Ca2+ in the submicromolar range against a very large electrochemical Ca2+ gradient across the plasma membrane, the ATP-driven Ca2+ pump and Na-Ca exchangers. Two types of Na-Ca exchangers are known, the 3Na:1Ca exchangers found in heart, brain, kidney, and most other tissues and the 4Na:1Ca+ 1K exchanger found in retinal rod and cone photoreceptors. Functional expression of Na-Ca(/K) exchangers is most often measured as 45Ca uptake in Na(+)-loaded cells or as Na-Ca exchange currents with the giant excised patch technique. In this study, two functional assays used to detect expression of the bovine heart Na-Ca exchanger in CHO cells are described. Both assays are based on measurements of cytosolic free Ca2+ with the fluorescent Ca(2+)-indicating dye fluo-3 and should be equally applicable in the study of functional expression of both Na-Ca and Na-Ca/K exchanger clones.

  8. CaMKII inhibition targeted to the sarcoplasmic reticulum inhibits frequency dependent acceleration of relaxation and Ca2+ current facilitation

    OpenAIRE

    Picht, Eckard; DeSantiago, Jaime; Huke, Sabine; Kaetzel, Marcia A.; Dedman, John R.; Bers, Donald M.

    2006-01-01

    Cardiac Ca2+/calmodulin-dependent protein kinase II (CaMKII) in heart has been implicated in Ca2+ current (ICa) facilitation, enhanced sarcoplasmic reticulum (SR) Ca2+ release and frequency dependent acceleration of relaxation (FDAR) via enhanced SR Ca2+ uptake. However, questions remain about how CaMKII may work in these three processes. Here we tested the role of CaM-KII in these processes using transgenic mice (SR-AIP) that express four concatenated repeats of the CaMKII inhibitory peptide...

  9. Treatment of Ruptured Ovarian Endometrioma with Extremely High CA 125, Moderately High CA 19-9 and CA 15-3 Level

    Directory of Open Access Journals (Sweden)

    Muzeyyen Duran

    2013-06-01

    Full Text Available In this case report, a ruptured ovarian endometrioma with a very high CA-125 level, moderately elevated CA 19-9, and CA 15-3 levels is presented. A 20 years old patient, complaining from pelvic pain, 5 cm adnexial mass was detected on left side. Biochemical examination was revealed very high CA-125 value (2556IU/ml, moderately elevated CA 19-9 (134IU/ml, and CA 15-3 (65IU/ml values. Laparoscopy was done and a ruptured ovarian endometrioma of 5 cm was seen during operation. After the total excision of the cyst, tumor markers fell rapidly. Very high CA-125 value, moderately elevated CA19-9, and CA 15-3 values can be seen in cases with ruptured endometrioma. In young patients, endometrioma must be considered firstly and laparoscopy should be applied instead of more invasive methods unless there was any finding or strong suspicion about malignancy.

  10. A significant but rather mild contribution of T286 autophosphorylation to Ca2+/CaM-stimulated CaMKII activity.

    Directory of Open Access Journals (Sweden)

    Steven J Coultrap

    Full Text Available BACKGROUND: Autophosphorylation of the Ca(2+/calmodulin (CaM-dependent protein kinase II (CaMKII at T286 generates partially Ca(2+/CaM-independent "autonomous" activity, which is thought to be required for long-term potentiation (LTP, a form of synaptic plasticity thought to underlie learning and memory. A requirement for T286 autophosphorylation also for efficient Ca(2+/CaM-stimulated CaMKII activity has been described, but remains controversial. METHODOLOGY/PRINCIPAL FINDINGS: In order to determine the contribution of T286 autophosphorylation to Ca(2+/CaM-stimulated CaMKII activity, the activity of CaMKII wild type and its phosphorylation-incompetent T286A mutant was compared. As the absolute activity can vary between individual kinase preparations, the activity was measured in six different extracts for each kinase (expressed in HEK-293 cells. Consistent with measurements on purified kinase (from a baculovirus/Sf9 cell expression system, CaMKII T286A showed a mildly but significantly reduced rate of Ca(2+/CaM-stimulated phosphorylation for two different peptide substrates (to ~75-84% of wild type. Additional slower CaMKII autophosphorylation at T305/306 inhibits stimulation by Ca(2+/CaM, but occurs only minimally for CaMKII wild type during CaM-stimulated activity assays. Thus, we tested if the T286A mutant may show more extensive inhibitory autophosphorylation, which could explain its reduced stimulated activity. By contrast, inhibitory autophosphorylation was instead found to be even further reduced for the T286A mutant under our assay conditions. On a side note, the phospho-T305 antibody showed some basal background immuno-reactivity also with non-phosphorylated CaMKII, as indicated by T305/306A mutants. CONCLUSIONS/SIGNIFICANCE: These results indicate that Ca(2+/CaM-stimulated CaMKII activity is mildly (~1.2-1.3fold further increased by additional T286 autophosphorylation, but that this autophosphorylation is not required for the major

  11. Control of ciliary motility by Ca sup 2+ : Integration of Ca sup 2+ -dependent functions and targets for Ca sup 2+ action

    Energy Technology Data Exchange (ETDEWEB)

    Evans, T.C.

    1988-01-01

    To identify functions that regulate Ca{sup 2+}-induced ciliary reversal in Paramecium, mutants defective in terminating depolarization-induced backward swimming were selected. Six independent recessive mutations (k-shy) comprising two complementation groups, k-shyA and k-shyB, were identified. All mutants exhibited prolonged backward swimming in depolarizing solutions. Voltage clamp studies revealed that mutant Ca{sup 2+} current amplitudes were reduced, but could be restored to wild type levels by EGTA injection. The recovery of the mutant Ca{sup 2+} current from Ca{sup 2+}-dependent inactivation, and the decay of the Ca{sup 2+}-dependent K{sup +} and Ca{sup 2+}-dependent Na{sup +} currents after depolarization were slow in k-shy compared to wild type. To identify protein targets of Ca{sup 2+} action, ciliary proteins that interact with calmodulin (CaM) were characterized. With a {sup 125}I-CaM blot assay, several CaM-binding proteins were identified including axonemal, soluble, and membrane-bound polypeptides. Competitive displacement studies with unlabeled Paramecium CaM, bovine CaM, and troponinC suggested that both protein types bind CaM with high affinity and specificity. To examine the presence of CaM-binding sites in intact axonemes, a filtration binding assay was developed.

  12. Diffusion of Ca and Mg in Calcite

    Energy Technology Data Exchange (ETDEWEB)

    Cygan, R.T.; Fisler, D.K.

    1999-02-10

    The self-diffusion of Ca and the tracer diffusion of Mg in calcite have been experimentally measured using isotopic tracers of {sup 25}Mg and {sup 44}Ca. Natural single crystals of calcite were coated with a thermally-sputtered oxide thin film and then annealed in a CO{sub 2} gas at one atmosphere total pressure and temperatures from 550 to 800 C. Diffusion coefficient values were derived from the depth profiles obtained by ion microprobe analysis. The resultant activation energies for Mg tracer diffusion and Ca self-diffusion are respectively: E{sub a}(Mg) = 284 {+-} 74 kJ/mol and E{sub a}(Ca) = 271 {+-} 80 kJ/mol. For the temperature ranges in these experiments, the diffusion of Mg is faster than Ca. The results are generally consistent in magnitude with divalent cation diffusion rates obtained in previous studies and provide a means of interpreting the thermal histories of carbonate minerals, the mechanism of dolomitization, and other diffusion-controlled processes. The results indicate that cation diffusion in calcite is relatively slow and cations are the rate-limiting diffusing species for the deformation of calcite and carbonate rocks. Application of the calcite-dolomite geothermometer to metamorphic assemblages will be constrained by cation diffusion and cooling rates. The direct measurement of Mg tracer diffusion in calcite indicates that dolomitization is unlikely to be accomplished by Mg diffusion in the solid state but by a recrystallization process.

  13. The Ever Flowing River Witness the History---TU Fu’ s Poetry and his poetic life%不废江河万古流--杜甫的诗歌与诗性人生

    Institute of Scientific and Technical Information of China (English)

    刘国庚

    2015-01-01

    唐代由盛及衰的离乱之世恰是中国诗坛群星璀璨的繁华时代。其中成就最为卓著、最具代表性的诗人,是“诗圣”杜甫。杜甫诗歌沉郁顿挫、兼容并蓄,其恒久魅力在于强烈的思想穿透力、人格亲和力以及诗中蕴藉的忧国忧民、悲天悯人的情怀和执著弘毅、挫而不折的品质。杜甫用一生的坚守和求索,筑起中国文学史上的一座圣殿,获得了崇高的历史地位。1200多年来,杜甫的诗风与人品,相映生辉,相得益彰,一直被知识阶层当作情感凭藉和行为楷模。%The periods of historic transformation in Tang dynasty shifting from prosperity to ruin happened to be the prosperous times of the famous and numerous composers of Chinese poetry.And among them the most distinguished and representative poet was Master Poet,Tu Fu.Tu Fu’ s poems were depressed,sonorous,and inclusive,and the e-ternal charm lied in his strong mental penetrating power and his character of affinity as well as his serious concern for his country,his people and his personality of perseverance embodied in his poems.With perseverant exploration throughout his life,he built up a great temple in the history of Chinese literature and achieved a lofty historical posi-tion.As Tu Fu’ s poetic style and moral quality complemented each other so much that he has long been taken as both emotional solace and role model for over 1 200 years.

  14. Clinical significance of detecting CEA, CA199, AFP, HCG, CA153, CA125 in postoperative treatment of patients with ovarian cancer

    Institute of Scientific and Technical Information of China (English)

    Xue Pan; Zhen-Hua Du

    2016-01-01

    Objective:To explore the clinical significance of carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), a tire protein (AFP), human chorionic gonadotropin (HCG), carbohydrate antigen 153 (CA153) and carbohydrate antigen 125 (CA125) in the postoperative treatment of patients with ovarian cancer.Methods:210 cases of patients with ovarian cancer after initial treatment from January 2015 to December 2015, 232 cases of patients with ovarian benign tumor and 250 cases of healthy women were selected, CEA, CA199, AFP, HCG, CA153 and CA125 levels were detected, and the levels after chemotherapy in patients with ovarian carcinoma were detected.Results:CEA, CA199, AFP, HCG, CA153 and CA125 levels in patients with ovarian cancer were (12.37±7.43) ng/mL, (84.04±26.96) U/mL, (37.46±9.47) μg/L, (110.54±35.51) IU/L, (51.23±9.20) U/mL and (64.36±42.68) U/mL, respectively, which were significantly higher than that in normal controls and patients with benign ovarian lesions, and were considered to be statistically significant. Chemotherapy after two cycles, CEA, CA199, AFP, HCG, CA153 and CA125 levels in patients with ovarian cancer were significantly lower than that before chemotherapy, and were considered to be statistically different. Chemotherapy after four cycles, CEA, CA199, AFP, HCG, CA153 and CA125 levels in patients with ovarian cancer continue to decrease, and were significantly lower than that of chemotherapy after two cycles, and had statistical differences.Conclusion:CEA, CA199, AFP, HCG, CA153 and CA125 can be used as important indicators for monitoring the chemotherapy effects, early recurrence and metastasis of ovarian cancer.

  15. Sulfide capacity of CaO-CaF2-SiO2 slags

    Science.gov (United States)

    Susaki, Katsujiro; Maeda, Masafumi; Sano, Nobuo

    1990-02-01

    The sulfide capacity C S 2- = (pct S2-) · ( P O 2/ P S 2)1/2) of CaO-CaF2-SiO2 slags saturated with CaO, 3CaO · SiO2 or 2CaOSiO2 was determined at 1200 °C, 1250 °C, 1300 °C, and 1350 °C by equilibrating molten slag, molten silver, and CO-CO2 gas mixtures. Higher sulfide capacities were obtained for CaO-saturated slags. A drastic decrease was observed in those values when the ratio pct CaO/pct SiO2 is less than 2. The sulfur partition between carbon-saturated iron melts and presently investigated slags was calculated by using the sulfide capacities obtained and the activity coefficient of sulfur in carbon-saturated iron, which was also experimentally determined. For slags saturated with CaO, partitions of sulfur as high as 10,000 were obtained at 1300 °C and 1350 °C. Correlations between the sulfide capacity and other basicity indexes such as carbonate capacity and theoretical optical basicity were also discussed.

  16. Multifaceted plasma membrane Ca(2+) pumps: From structure to intracellular Ca(2+) handling and cancer.

    Science.gov (United States)

    Padányi, Rita; Pászty, Katalin; Hegedűs, Luca; Varga, Karolina; Papp, Béla; Penniston, John T; Enyedi, Ágnes

    2016-06-01

    Plasma membrane Ca(2+) ATPases (PMCAs) are intimately involved in the control of intracellular Ca(2+) concentration. They reduce Ca(2+) in the cytosol not only by direct ejection, but also by controlling the formation of inositol-1,4,5-trisphosphate and decreasing Ca(2+) release from the endoplasmic reticulum Ca(2+) pool. In mammals four genes (PMCA1-4) are expressed, and alternative RNA splicing generates more than twenty variants. The variants differ in their regulatory characteristics. They localize into highly specialized membrane compartments and respond to the incoming Ca(2+) with distinct temporal resolution. The expression pattern of variants depends on cell type; a change in this pattern can result in perturbed Ca(2+) homeostasis and thus altered cell function. Indeed, PMCAs undergo remarkable changes in their expression pattern during tumorigenesis that might significantly contribute to the unbalanced Ca(2+) homeostasis of cancer cells. This article is part of a Special Issue entitled: Calcium and Cell Fate. Guest Editors: Jacques Haiech, Claus Heizmann, Joachim Krebs, Thierry Capiod and Olivier Mignen.

  17. Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.

    Science.gov (United States)

    Hollingworth, Stephen; Gee, Kyle R; Baylor, Stephen M

    2009-10-07

    The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly track Delta[Ca(2+)]). In response to an action potential, the full duration at half-maximum of the indicator's fluorescence change (DeltaF) was found to be larger with OGB-5N, Fluo-5N, fura-5N, and Rhod-5N than with furaptra; thus, these indicators do not track Delta[Ca(2+)] with kinetic fidelity. In contrast, the DeltaF time course of Mag-fluo-4 was identical to furaptra's; thus, Mag-fluo-4 also yields reliable kinetic information about Delta[Ca(2+)]. Mag-fluo-4's DeltaF has a larger signal/noise ratio than furaptra's (for similar indicator concentrations), and should thus be more useful for tracking Delta[Ca(2+)] in small cell volumes. However, because the resting fluorescence of Mag-fluo-4 probably arises largely from indicator that is bound with Mg(2+), the amplitude of the Mag-fluo-4 signal, and its calibration in Delta[Ca(2+)] units, is likely to be more sensitive to variations in [Mg(2+)] than furaptra's.

  18. Investigation of the 42Ca(n, γ)43Ca reaction

    NARCIS (Netherlands)

    Gruppelaar, H.; Kamp, A.M.F. op den; Spits, A.M.J.

    1969-01-01

    Gamma rays from thermal-neutron capture in enriched 42Ca were investigated with Ge(Li) detectors. Gamma-gamma coincidence measurements were performed with a Ge(Li)---NaI detector combination. A decay scheme of 43Ca has been constructed containing 42 of the 47 observed γ-lines. The excitation energie

  19. Synaptotagmin-7 is a principal Ca2+ sensor for Ca2+ -induced glucagon exocytosis in pancreas

    DEFF Research Database (Denmark)

    Gustavsson, Natalia; Wei, Shun-Hui; Hoang, Dong Nhut

    2009-01-01

    abolished Ca(2+)-triggered glucagon secretion. Moreover, single-cell capacitance measurements confirmed that pancreatic alpha-cells lacking synaptotagmin-7 exhibited little Ca(2+)-induced exocytosis, whereas all other physiological and morphological parameters of the alpha-cells were normal. Our data thus...

  20. (Meta)stable CaC{sub 2}; (Meta)stabiles CaC{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Glaser, J.; Dill, S.; Marzini, M.; Mayer, H.A.; Meyer, H.J. [Tuebingen Univ. (Germany). Inst. fuer Anorganische Chemie

    2001-05-01

    One out of four modifications of CaC{sub 2} is the so-called metastable calcium carbide, CaC{sub 2}-III, which was synthesized as pure material. It forms by heating monoclinic CaC{sub 2}-II (C2/c) above 150 C and remains stable after cooling down to room temperature. The structure was refined from X-ray powder patterns (C2/m, Z = 4, a = 722.6(1) pm, b = 385.26(7) pm, c = 737.6(1) pm, {beta} = 107.345(2) ). After grinding CaC{sub 2}-III transforms back into CaC{sub 2}-II. Heating CaC{sub 2}-III induces a reversible phase transition into the cubic modification (CaC{sub 2}-IV) at 460 C. Differences between the three different structures of CaC{sub 2} I-III, being stable at ambient conditions are also shown by {sup 13}C-MAS-NMR measurements, especially the presence of two distinct types of carbon atoms in the structure of the title compound. (orig.)

  1. Differential NMDA receptor-dependent calcium loading and mitochondrial dysfunction in CA1 vs. CA3 hippocampal neurons.

    Science.gov (United States)

    Stanika, Ruslan I; Winters, Christine A; Pivovarova, Natalia B; Andrews, S Brian

    2010-02-01

    Hippocampal CA1 pyramidal neurons are selectively vulnerable to ischemia, while adjacent CA3 neurons are relatively resistant. Although glutamate receptor-mediated mitochondrial Ca(2+) overload and dysfunction is a major component of ischemia-induced neuronal death, no direct relationship between selective neuronal vulnerability and mitochondrial dysfunction has been demonstrated in intact brain preparations. Here, we show that in organotypic slice cultures NMDA induces much larger Ca(2+) elevations in vulnerable CA1 neurons than in resistant CA3. Consequently, CA1 mitochondria exhibit stronger calcium accumulation, more extensive swelling and damage, stronger depolarization of their membrane potential, and a significant increase in ROS generation. NMDA-induced Ca(2+) and ROS elevations were abolished in Ca(2+)-free medium or by NMDAR antagonists, but not by zinc chelation. We conclude that Ca(2)(+) overload-dependent mitochondrial dysfunction is a determining factor in the selective vulnerability of CA1 neurons.

  2. Atom Trap Trace Analysis of Ca Isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Hoekstra, S., E-mail: hoekstra@fhi-berlin.mgp.de [Fritz-Haber Institut der Max-Planck Gesellschaft (Germany); Mollema, A. K.; Morgenstern, R.; Willmann, L.; Wilschut, H. W.; Hoekstra, R. [Rijksuniversiteit Groningen, Atomic Physics, KVI (Netherlands)

    2005-04-15

    In our experiment we aim at the detection of the rarest, naturally occuring calcium isotope 41Ca by means of atom trap trace analysis. On basis of single-atom detection of 46Ca our present sensitivity for 41Ca is estimated to be 1 atom per hour at an abundance of 10-12. To reach a sensitivity at the level of natural abundance, which is 10-14, we need to reduce atomic beam losses. To achieve this, optical compression of the atomic beam is a promising option. We use Monte Carlo Simulations to demonstrate that optical compression of the atomic beam increases throughput of the atomic beam as well as isotope selectivity.

  3. Intercellular Ca2+ Waves: Mechanisms and Function

    Science.gov (United States)

    Sanderson, Michael J.

    2012-01-01

    Intercellular calcium (Ca2+) waves (ICWs) represent the propagation of increases in intracellular Ca2+ through a syncytium of cells and appear to be a fundamental mechanism for coordinating multicellular responses. ICWs occur in a wide diversity of cells and have been extensively studied in vitro. More recent studies focus on ICWs in vivo. ICWs are triggered by a variety of stimuli and involve the release of Ca2+ from internal stores. The propagation of ICWs predominately involves cell communication with internal messengers moving via gap junctions or extracellular messengers mediating paracrine signaling. ICWs appear to be important in both normal physiology as well as pathophysiological processes in a variety of organs and tissues including brain, liver, retina, cochlea, and vascular tissue. We review here the mechanisms of initiation and propagation of ICWs, the key intra- and extracellular messengers (inositol 1,4,5-trisphosphate and ATP) mediating ICWs, and the proposed physiological functions of ICWs. PMID:22811430

  4. ASteCA - Automated Stellar Cluster Analysis

    CERN Document Server

    Perren, Gabriel I; Piatti, Andrés E

    2014-01-01

    We present ASteCA (Automated Stellar Cluster Analysis), a suit of tools designed to fully automatize the standard tests applied on stellar clusters to determine their basic parameters. The set of functions included in the code make use of positional and photometric data to obtain precise and objective values for a given cluster's center coordinates, radius, luminosity function and integrated color magnitude, as well as characterizing through a statistical estimator its probability of being a true physical cluster rather than a random overdensity of field stars. ASteCA incorporates a Bayesian field star decontamination algorithm capable of assigning membership probabilities using photometric data alone. An isochrone fitting process based on the generation of synthetic clusters from theoretical isochrones and selection of the best fit through a genetic algorithm is also present, which allows ASteCA to provide accurate estimates for a cluster's metallicity, age, extinction and distance values along with its unce...

  5. Ca3Mn2O7.

    Science.gov (United States)

    Guiblin, Nicolas; Grebille, Dominique; Leligny, Henri; Martin, Christine

    2002-01-01

    The tricalcium dimanganese heptaoxide (Ca3Mn2O7) member of the Ruddlesden-Popper series Ca(n+1)Mn(n)O(3n+1), i.e. with n = 2, was previously reported with an I-centred tetragonal lattice [a(t) = 3.68 and c(t) = 19.57 A] by Fawcett, Sunstrom, Greenblatt, Croft & Ramanujachary [Chem. Mater. (1998), 10, 3643-3651]. It is now found to be orthorhombic, with an A-centred lattice [a = 5.2347 (6), b = 5.2421 (2) and c = 19.4177 (19) A]. The structure has been refined in space group A2(1)am using X-ray single-crystal diffraction data and assuming the existence of twin domains related by the (1-10) plane. A comparison with the basic perovskite structure CaMnO3 (n = infinity) is proposed.

  6. Calcium platinum aluminium, CaPtAl

    Directory of Open Access Journals (Sweden)

    Charles Fon Abi

    2011-10-01

    Full Text Available A preliminary X-ray study of CaPtAl has been reported previously by Hulliger [J. Alloys Compd (1993, 196, 225–228] based on X-ray powder diffraction data without structure refinement. With the present single-crystal X-ray study, we confirm the assignment of the TiNiSi type for CaPtAl, in a fully ordered inverse structure. All three atoms of the asymmetric unit have .m. site symmetry. The structure features a ∞3[AlPt] open framework with a fourfold coordination of Pt by Al atoms and vice versa. The Ca atoms are located in the large channels of the structure.

  7. Anoxia-induced elevation of cytosolic Ca2+ concentration depends on different Ca2+ sources in rice and wheat protoplasts.

    Science.gov (United States)

    Yemelyanov, Vladislav V; Shishova, Maria F; Chirkova, Tamara V; Lindberg, Sylvia M

    2011-08-01

    The anoxia-dependent elevation of cytosolic Ca(2+) concentration, [Ca(2+)](cyt), was investigated in plants differing in tolerance to hypoxia. The [Ca(2+)](cyt) was measured by fluorescence microscopy in single protoplasts loaded with the calcium-fluoroprobe Fura 2-AM. Imposition of anoxia led to a fast (within 3 min) significant elevation of [Ca(2+)](cyt) in rice leaf protoplasts. A tenfold drop in the external Ca(2+) concentration (to 0.1 mM) resulted in considerable decrease of the [Ca(2+)](cyt) shift. Rice root protoplasts reacted upon anoxia with higher amplitude. Addition of plasma membrane (verapamil, La(3+) and EGTA) and intracellular membrane Ca(2+)-channel antagonists (Li(+), ruthenium red and cyclosporine A) reduced the anoxic Ca(2+)-accumulation in rice. Wheat protoplasts responded to anoxia by smaller changes of [Ca(2+)](cyt). In wheat leaf protoplasts, the amplitude of the Ca(2+)-shift little depended on the external level of Ca(2+). Wheat root protoplasts were characterized by a small shift of [Ca(2+)](cyt) under anoxia. Plasmalemma Ca(2+)-channel blockers had little effect on the elevation of cytosolic Ca(2+) in wheat protoplasts. Intact rice seedlings absorbed Ca(2+) from the external medium under anoxic treatment. On the contrary, wheat seedlings were characterized by leakage of Ca(2+). Verapamil abolished the Ca(2+) influx in rice roots and Ca(2+) efflux from wheat roots. Anoxia-induced [Ca(2+)](cyt) elevation was high particularly in rice, a hypoxia-tolerant species. In conclusion, both external and internal Ca(2+) stores are important for anoxic [Ca(2+)](cyt) elevation in rice, whereas the hypoxia-intolerant wheat does not require external sources for [Ca(2+)](cyt) rise. Leaf and root protoplasts similarly responded to anoxia, independent of their organ origin.

  8. Mitochondrial Ca(2+) uptake in skeletal muscle health and disease.

    Science.gov (United States)

    Zhou, Jingsong; Dhakal, Kamal; Yi, Jianxun

    2016-08-01

    Muscle uses Ca(2+) as a messenger to control contraction and relies on ATP to maintain the intracellular Ca(2+) homeostasis. Mitochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca(2+) from their surroundings, a process called mitochondrial Ca(2+) uptake. Under physiological conditions, Ca(2+) uptake into mitochondria promotes ATP production. Excessive uptake causes mitochondrial Ca(2+) overload, which activates downstream adverse responses leading to cell dysfunction. Moreover, mitochondrial Ca(2+) uptake could shape spatio-temporal patterns of intracellular Ca(2+) signaling. Malfunction of mitochondrial Ca(2+) uptake is implicated in muscle degeneration. Unlike non-excitable cells, mitochondria in muscle cells experience dramatic changes of intracellular Ca(2+) levels. Besides the sudden elevation of Ca(2+) level induced by action potentials, Ca(2+) transients in muscle cells can be as short as a few milliseconds during a single twitch or as long as minutes during tetanic contraction, which raises the question whether mitochondrial Ca(2+) uptake is fast and big enough to shape intracellular Ca(2+) signaling during excitation-contraction coupling and creates technical challenges for quantification of the dynamic changes of Ca(2+) inside mitochondria. This review focuses on characterization of mitochondrial Ca(2+) uptake in skeletal muscle and its role in muscle physiology and diseases.

  9. Electrochemical formation of Mg-Li-Ca alloys by codeposition of Mg, Li and Ca from LiCl-KCl-MgCl2-CaCl2 melts.

    Science.gov (United States)

    Yan, Yong De; Zhang, Mi Lin; Xue, Yun; Han, Wei; Cao, Dian Xue; Jing, Xiao Yan; He, Li Yi; Yuan, Yi

    2009-08-07

    This work presents electrochemical formation of Mg-Li-Ca alloys via codeposition of Mg, Li and Ca on a molybdenum electrode in KCl-LiCl-MgCl(2)-CaCl(2) melts at 943 K. Cyclic voltammograms (CVs) showed that the underpotential deposition (UPD) of calcium on pre-deposited magnesium leads to the formation of a liquid Mg-Ca alloy, and the succeeding underpotential deposition of lithium on pre-deposited Mg-Ca alloy leads to the formation of a liquid Mg-Li-Ca solution. Chronopotentiometric measurements indicated that the codepositon of Mg, Li and Ca occurs at current densities more negative than -0.31 A cm(-2) in LiCl-KCl-MgCl(2) (5 wt%) melts containing 1 wt% CaCl(2). Chronoamperograms demonstrated that the onset potential for the codeposition of Mg, Li and Ca is -2.200 V, and the codeposition of Mg, Li and Ca is formed when the applied potentials are more negative than -2.200 V. X-Ray diffraction (XRD) indicated that Mg-Li-Ca alloys with different phases were formed via galvanostatic electrolysis. The microstructures of typical alpha and beta phases of Mg-Li-Ca alloys were characterized by optical microscope (OM) and scanning electron microscopy (SEM). The analysis of energy dispersive spectrometry (EDS) showed that the element Ca mainly distributes along grain boundary in Mg-Li-Ca alloys. The results of inductively coupled plasma analysis determined that the chemical compositions of Mg-Li-Ca alloys correspond with the phase structures of XRD patterns, and the lithium and calcium contents of Mg-Li-Ca alloys depend on the concentrations of MgCl(2) and CaCl(2).

  10. Starppersonu komunikācijas veida saistība ar pusaudžu pašvērtējumu un vientulības izjūtu

    OpenAIRE

    Kreitūze, Ieva

    2016-01-01

    Pētījuma mērķis ir noskaidrot vai pusaudžu komunikācija ir saistīta ar pusaudžu pašvērtējumu un vientulības izjūtu. Pētījuma mērķa sasniegšanai tika izmantota Rozenberga pašvērtējuma skala, (Rosenberg Self-Esteem Scale, Rosenberg, 1965), UCLA vientulības skala, (The Revised UCLA Loneliness Scale, Russell, Peplau, & Cutrona, 1996). Komunikācijas izpētei tika izmantota Interpersonālās komunikācijas skala, (Interpersonal Communication Inventory, Millard & Bienvenu, 1971 Interpersonal Communicat...

  11. Starppersonu komunikācijas veida saistība ar pusaudžu pašvērtējumu un vientulības izjūtu

    OpenAIRE

    Kreitūze, Ieva

    2016-01-01

    Pētījuma mērķis ir noskaidrot vai pusaudžu komunikācija ir saistīta ar pusaudžu pašvērtējumu un vientulības izjūtu. Pētījuma mērķa sasniegšanai tika izmantota Rozenberga pašvērtējuma skala, (Rosenberg Self-Esteem Scale, Rosenberg, 1965), UCLA vientulības skala, (The Revised UCLA Loneliness Scale, Russell, Peplau, & Cutrona, 1996). Komunikācijas izpētei tika izmantota Interpersonālās komunikācijas skala, (Interpersonal Communication Inventory, Millard & Bienvenu, 1971 Interpersonal Communicat...

  12. Gallija-68 [68Ga] un fluora-18 [18F] izotopu iegūšana un izmantošana radiofarmaceitisko preparātu sintēzē

    OpenAIRE

    Mamis, Edgars

    2017-01-01

    Gallija-68 [68Ga] un fluora-18 [18F] izotopu iegūšana un izmantošana radiofarmaceitisko preparātu sintēzē. Mamis E., zinātniskais vadītājs Prof. Dr.h.c. Chem Andris Actiņš, zinātniskie konsultanti Mg. Chem Jāzeps Malnačs un Mg. Med. Phys., Dipl. Ing., Mg. Sc. Ing Linda Meistere. Maģistra darbs, 54 lappuses, 28 attēli, 12 tabulas, 20 literatūras avoti. Latviešu valodā. Darbā ir apkopota literatūra par radionuklīdu iegūšanas procesiem kodolreakcijas ceļā, izmantojot daļiņu paātrinātāju – ciklo...

  13. CA19-9antigenlevelscandistinguishbetween benignandmalignantpancreaticobiliarydisease

    Institute of Scientific and Technical Information of China (English)

    Gareth Morris-Stiff; Mary Teli; Nicky Jardine; Malcolm CA Puntis

    2009-01-01

    BACKGROUND: CA19-9 is a carbohydrate tumor-associated antigen which is frequently upregulated in pancreatobiliary neoplasia. However, it may also be elevated in patients with jaundice in the absence of a tumor due to biliary obstruction, and in other non-hepato-pancreatico-biliary conditions. This study aimed to evaluate whether CA19-9 levels could accurately differentiate between benign and malignant pancreatobiliary disease. METHODS: All patients referred to a single surgeon for investigation of pancreaticobiliary disease in 2003 in whom a ifrm diagnosis had been established were included. For malignant disease, a histological diagnosis was required but for benign disease a ifrm radiological diagnosis was deemed adequate. The patients were divided into 4 categories:pancreatic adenocarcinoma (PCa); cholangiocarcinoma (CCa); chronic pancreatitis (CP) and biliary calculous disease (Calc). Bilirubin and alkaline phosphatase levels corresponding to the point of assessment of CA19-9 were also noted. RESULTS:  Final diagnoses were made of pancreatic adenocarcinoma (PCa, n=73), cholangiocarcinoma (CCa, n=19), ampullary carcinoma (Amp, n=7), neuroendocrine carcinoma (Neu, n=4), duodenal carcinoma (Duo, n=3), chronic pancreatitis (CP, n=115), and biliary calculous disease (Calc, n=27). Median CA19-9 levels (U/ml) were:PCa, 653;CCa, 408;Duo, 403;Calc, 27;CP, 19;Neu, 10.5;Amp, 8 (reference range: 0-37). The CA19-9 levels were signiifcantly greater for malignant than for benign disease, could differentiate PCa from CCa/Duo, and were signiifcantly higher in unresectable than in resectable PCa. The sensitivity, speciifcity, positive predictive value (PPV) and negative predictive value (NPV) for CA19-9 were 84.9%, 69.7%, 67.7%and 86.1%, respectively. A ROC analysis provided an area under the curve for CA19-9 of 0.871 (0.820-0.922), giving an optimal CA19-9 of 70.5 U/ml for differentiating benign from malignant pathology. Using this cut-off, the sensitivity was 82

  14. 大鼠海马CA2区GDNF mRNA表达与吗啡依赖和吗啡的关系%Involvement of Hippocampus in the Development of Morphine-withdrawal Rats

    Institute of Scientific and Technical Information of China (English)

    牟燕; 吕志平

    2008-01-01

    [目的]观察吗啡戒断大鼠侧脑室胶质细胞源性神经营养因子(GDNF)反义寡脱氧核苷酸注射后对海马GDNF mRNA表达的影响.[方法]SD大鼠侧脑室立体定位,皮下注射吗啡建立吗啡依赖大鼠模型,侧脑室微注射GDNF有义和反义寡脱氧核苷酸,纳洛酮催促戒断,应用原位杂交方法检测海马GDNF mRNA的表迭.[结果]侧脑室注射GDNF反义寡脱氧核苷酸能显著抑制大鼠吗啡戒断症状评分(P0.05);吗啡依赖大鼠纳洛酮激发前24 h侧脑室注射GDNF反义寡脱氧核苷酸能显著抑制吗啡依赖大鼠依赖和戒断后CA2区GDNF tuRNA表达的增加(P0.05).[结论]在转录水平,海马CA2区GDNF表达的变化在吗啡依赖和戒断中可能起重要作用.

  15. Novel regulatory aspects of the extracellular Ca2+-sensing receptor, CaR.

    Science.gov (United States)

    Riccardi, Daniela; Finney, Brenda A; Wilkinson, William J; Kemp, Paul J

    2009-10-01

    The capacity to sense and adapt to changes in environmental cues is of paramount importance for every living organism. From yeast to man, cells must be able to match cellular activities to growth environment and nutrient availability. Key to this process is the development of membrane-bound systems that can detect modifications in the extracellular environment and to translate these into biological responses. Evidence gathered over the last 15 years has demonstrated that many of these cell surface "sensors" belong to the G protein-coupled receptor superfamily. Crucial to our understanding of nutrient sensing in mammalian species has been the identification of the extracellular Ca(2+)/cation-sensing receptor, CaR. CaR was the first ion-sensing molecule identified in man and genetic studies in humans have revealed the importance of the CaR in mineral ion metabolism. Latter, it has become apparent that the CaR also plays an important role outside the Ca(2+) homeostatic system, as an integrator of multiple environmental signals for the regulation of many vital cellular processes, from cell-to-cell communication to secretion and cell survival/cell death. Recently, novel aspects of receptor function reveal an unexpected role for the CaR in the regulation of growth and development in utero.

  16. Ca(2+)-activated chloride channel activity during Ca(2+) alternans in ventricular myocytes.

    Science.gov (United States)

    Kanaporis, Giedrius; Blatter, Lothar A

    2016-11-01

    Cardiac alternans, defined beat-to-beat alternations in contraction, action potential (AP) morphology or cytosolic Ca transient (CaT) amplitude, is a high risk indicator for cardiac arrhythmias. We investigated mechanisms of cardiac alternans in single rabbit ventricular myocytes. CaTs were monitored simultaneously with membrane currents or APs recorded with the patch clamp technique. A strong correlation between beat-to-beat alternations of AP morphology and CaT alternans was observed. During CaT alternans application of voltage clamp protocols in form of pre-recorded APs revealed a prominent Ca(2+)-dependent membrane current consisting of a large outward component coinciding with AP phases 1 and 2, followed by an inward current during AP repolarization. Approximately 85% of the initial outward current was blocked by Cl(-) channel blocker DIDS or lowering external Cl(-) concentration identifying it as a Ca(2+)-activated Cl(-) current (ICaCC). The data suggest that ICaCC plays a critical role in shaping beat-to-beat alternations in AP morphology during alternans.

  17. DA-6034 Induces [Ca(2+)]i Increase in Epithelial Cells.

    Science.gov (United States)

    Yang, Yu-Mi; Park, Soonhong; Ji, Hyewon; Kim, Tae-Im; Kim, Eung Kweon; Kang, Kyung Koo; Shin, Dong Min

    2014-04-01

    DA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca(2+) signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca(2+) signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca(2+)-activated Cl(-) channels (CaCCs) and increased intracellular calcium concentrations ([Ca(2+)]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca(2+)]i in mouse salivary gland cells and human corneal epithelial cells. [Ca(2+)]i increase of DA-6034 was dependent on the Ca(2+) entry from extracellular and Ca(2+) release from internal Ca(2+) stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca(2+) stores. These results suggest that DA-6034 induces Ca(2+) signaling via extracellular Ca(2+) entry and RyRs-sensitive Ca(2+) release from internal Ca(2+) stores in epithelial cells.

  18. 75 FR 8414 - California Disaster # CA-00150

    Science.gov (United States)

    2010-02-24

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00150 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated...

  19. 76 FR 7622 - California Disaster #CA-00162

    Science.gov (United States)

    2011-02-10

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00162 AGENCY: U.S. Small Business Administration. ] ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated...

  20. 77 FR 61815 - California Disaster #CA-00190

    Science.gov (United States)

    2012-10-11

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00190 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of the Administrative declaration of a disaster for the State of...

  1. 76 FR 38263 - California Disaster #CA-00172

    Science.gov (United States)

    2011-06-29

    ... From the Federal Register Online via the Government Publishing Office Small Business Administration California Disaster CA-00172 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  2. 75 FR 17792 - California Disaster # CA-00150

    Science.gov (United States)

    2010-04-07

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00150 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of the Administrative declaration of disaster for the State of...

  3. 76 FR 5856 - California Disaster #CA-00164

    Science.gov (United States)

    2011-02-02

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00164 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  4. 75 FR 13144 - California Disaster #CA-00151

    Science.gov (United States)

    2010-03-18

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00151 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  5. 76 FR 11307 - California Disaster #CA-00162

    Science.gov (United States)

    2011-03-01

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00162 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of the Administrative declaration of a disaster for the State of...

  6. 75 FR 68848 - California Disaster #CA-00160

    Science.gov (United States)

    2010-11-09

    ... [Federal Register Volume 75, Number 216 (Tuesday, November 9, 2010)] [Notices] [Pages 68848-68849] [FR Doc No: 2010-28201] SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12370 and 12371] California Disaster CA-00160 AGENCY: U.S. Small Business Administration. [[Page 68849

  7. 76 FR 18614 - California Disaster #CA-00167

    Science.gov (United States)

    2011-04-04

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00167 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated...

  8. 76 FR 80446 - California Disaster #CA-00182

    Science.gov (United States)

    2011-12-23

    ... [Federal Register Volume 76, Number 247 (Friday, December 23, 2011)] [Notices] [Pages 80446-80447] [FR Doc No: 2011-32952] SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12967 and 12968] California Disaster CA-00182 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is...

  9. 76 FR 24555 - California Disaster #CA-00171

    Science.gov (United States)

    2011-05-02

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00171 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of CALIFORNIA dated...

  10. 77 FR 58901 - California Disaster #CA-00190

    Science.gov (United States)

    2012-09-24

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00190 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated...

  11. 75 FR 27846 - California Disaster # CA-00155

    Science.gov (United States)

    2010-05-18

    ... [Federal Register Volume 75, Number 95 (Tuesday, May 18, 2010)] [Notices] [Page 27846] [FR Doc No: 2010-11746] SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12166 and 12167] California Disaster CA-00155 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of...

  12. 75 FR 69733 - California Disaster #CA-00161

    Science.gov (United States)

    2010-11-15

    ... [Federal Register Volume 75, Number 219 (Monday, November 15, 2010)] [Notices] [Page 69733] [FR Doc No: 2010-28587] SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12373 and 12374] California Disaster CA-00161 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice...

  13. 75 FR 22872 - California Disaster # CA-00154

    Science.gov (United States)

    2010-04-30

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION California Disaster CA-00154 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated...

  14. Numerical model of Ca(OH)

    NARCIS (Netherlands)

    Koster, T.; Peelen, W.; Larbi, J.; Rooij, M. de; Polder, R.

    2010-01-01

    A mathematical model is being developed to describe a repair method in concrete, called cathodic protection (CP). The model is in principle also useful to describe electrodeposition in concrete, e.g. the process of re-precipitation of Ca(OH)2 invoked by an electrical current. In CP, the c

  15. 76 FR 20433 - California Disaster #CA-00169

    Science.gov (United States)

    2011-04-12

    ... ADMINISTRATION California Disaster CA-00169 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 04/05/2011. Incident: Center Fire. Incident Period: 03/18/2011 through 03/20/2011. Effective Date:...

  16. 78 FR 55771 - California Disaster #CA-00207

    Science.gov (United States)

    2013-09-11

    ... ADMINISTRATION California Disaster CA-00207 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of CALIFORNIA dated 08/26/2013. Incident: Silver Fire. Incident Period: 08/07/2013 through 08/14/2013. Effective Date:...

  17. 76 FR 62132 - California Disaster #CA-00176

    Science.gov (United States)

    2011-10-06

    ... ADMINISTRATION California Disaster CA-00176 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 09/29/2011. Incident: Canyon Fire. Incident Period: 09/04/2011 through 09/11/2011. Effective Date:...

  18. 78 FR 60366 - California Disaster #CA-00212

    Science.gov (United States)

    2013-10-01

    ... ADMINISTRATION California Disaster CA-00212 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 09/24/2013. Incident: Clover Fire Incident Period: 09/09/2013 through 09/15/2013. Effective Date:...

  19. 77 FR 1971 - California Disaster #CA-00183

    Science.gov (United States)

    2012-01-12

    ... ADMINISTRATION California Disaster CA-00183 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 01/05/2012. Incident: 1502 Golden Gate Fire. Incident Period: 12/22/2011. Effective Date:...

  20. 78 FR 39821 - California Disaster #CA-00202

    Science.gov (United States)

    2013-07-02

    ... ADMINISTRATION California Disaster CA-00202 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 06/25/2013. Incident: Powerhouse Fire. Incident Period: 05/30/2013 through 06/11/2013. Effective Date:...

  1. 78 FR 77195 - California Disaster #CA-00214

    Science.gov (United States)

    2013-12-20

    ... ADMINISTRATION California Disaster CA-00214 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the State of California (FEMA- 4158-DR), dated 12/13/2013. Incident: Rim Fire. Incident Period:...

  2. 76 FR 16029 - CALIFORNIA Disaster #CA-00165

    Science.gov (United States)

    2011-03-22

    ... ADMINISTRATION CALIFORNIA Disaster CA-00165 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of CALIFORNIA dated 03/16/2011. Incident: Garden Breeze Apartment Complex Fire. Incident Period: 02/20/2011. DATES:...

  3. 76 FR 79751 - California Disaster #CA-00181

    Science.gov (United States)

    2011-12-22

    ... ADMINISTRATION California Disaster CA-00181 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of California dated 12/14/2011. Incident: Sequoia Apartment Complex Fire. Incident Period: 11/18/2011. Effective Date:...

  4. Hippocampal CA1 Ripples as Inhibitory Transients.

    Directory of Open Access Journals (Sweden)

    Paola Malerba

    2016-04-01

    Full Text Available Memories are stored and consolidated as a result of a dialogue between the hippocampus and cortex during sleep. Neurons active during behavior reactivate in both structures during sleep, in conjunction with characteristic brain oscillations that may form the neural substrate of memory consolidation. In the hippocampus, replay occurs within sharp wave-ripples: short bouts of high-frequency activity in area CA1 caused by excitatory activation from area CA3. In this work, we develop a computational model of ripple generation, motivated by in vivo rat data showing that ripples have a broad frequency distribution, exponential inter-arrival times and yet highly non-variable durations. Our study predicts that ripples are not persistent oscillations but result from a transient network behavior, induced by input from CA3, in which the high frequency synchronous firing of perisomatic interneurons does not depend on the time scale of synaptic inhibition. We found that noise-induced loss of synchrony among CA1 interneurons dynamically constrains individual ripple duration. Our study proposes a novel mechanism of hippocampal ripple generation consistent with a broad range of experimental data, and highlights the role of noise in regulating the duration of input-driven oscillatory spiking in an inhibitory network.

  5. Hippocampal CA1 Ripples as Inhibitory Transients.

    Science.gov (United States)

    Malerba, Paola; Krishnan, Giri P; Fellous, Jean-Marc; Bazhenov, Maxim

    2016-04-01

    Memories are stored and consolidated as a result of a dialogue between the hippocampus and cortex during sleep. Neurons active during behavior reactivate in both structures during sleep, in conjunction with characteristic brain oscillations that may form the neural substrate of memory consolidation. In the hippocampus, replay occurs within sharp wave-ripples: short bouts of high-frequency activity in area CA1 caused by excitatory activation from area CA3. In this work, we develop a computational model of ripple generation, motivated by in vivo rat data showing that ripples have a broad frequency distribution, exponential inter-arrival times and yet highly non-variable durations. Our study predicts that ripples are not persistent oscillations but result from a transient network behavior, induced by input from CA3, in which the high frequency synchronous firing of perisomatic interneurons does not depend on the time scale of synaptic inhibition. We found that noise-induced loss of synchrony among CA1 interneurons dynamically constrains individual ripple duration. Our study proposes a novel mechanism of hippocampal ripple generation consistent with a broad range of experimental data, and highlights the role of noise in regulating the duration of input-driven oscillatory spiking in an inhibitory network.

  6. literacy.ca EXPRESS. April 2010

    Science.gov (United States)

    Movement for Canadian Literacy, 2010

    2010-01-01

    This issue of "literacy.ca EXPRESS" focuses on poverty. The articles included in this issue are: (1) Poverty Overview; (2) Tony's Story; (3) LAN (Learner Advisory Network) Member's Story (Dianne Smith); (4) Linking Adult Literacy to Poverty Reduction; (5) MCL (Movement for Canadian Literacy) Update; (6) Highlights from the LAN; (7) Good…

  7. Serum concentrations of the biomarkers CA125, CA15-3, CA72-4, tPSA and PAPP-A in natural and stimulated ovarian cycles

    Directory of Open Access Journals (Sweden)

    Melissa Stemp

    2014-06-01

    Conclusion: Ovarian stimulation reduced serum PAPP‐A levels, CA125 and CA15‐3 levels were generally unaffected by ovarian stimulation but displayed cyclical changes throughout both natural and stimulated cycles, whilst tPSA and CA72-4 were not affected by the stage of the cycle or ovarian stimulation.

  8. Cenozoic seawater Sr/Ca evolution

    Science.gov (United States)

    Sosdian, Sindia M.; Lear, Caroline H.; Tao, Kai; Grossman, Ethan L.; O'Dea, Aaron; Rosenthal, Yair

    2012-10-01

    Records of seawater chemistry help constrain temporal variations in geochemical processes that impact the global carbon cycle and climate through Earth's history. Here we reconstruct Cenozoic seawater Sr/Ca (Sr/Casw) using fossil Conus and turritellid gastropod Sr/Ca. Combined with an oxygen isotope paleotemperature record from the same samples, the gastropod record suggests that Sr/Caswwas slightly higher in the Eocene (˜11.4 ± 3 mmol/mol) than today (˜8.54 mmol/mol) and remained relatively stable from the mid- to late Cenozoic. We compare our gastropod Cenozoic Sr/Casw record with a published turritellid gastropod Sr/Casw record and other published biogenic (benthic foraminifera, fossil fish teeth) and inorganic precipitate (calcite veins) Sr/Caswrecords. Once the uncertainties with our gastropod-derived Sr/Casw are taken into account the Sr/Casw record agrees reasonably well with biogenic Sr/Caswrecords. Assuming a seawater [Ca] history derived from marine evaporite inclusions, all biogenic-based Sr/Casw reconstructions imply decreasing seawater [Sr] through the Cenozoic, whereas the calcite vein Sr/Casw reconstruction implies increasing [Sr] through the Cenozoic. We apply a simple geochemical model to examine the implications of divergence among these seawater [Sr] reconstructions and suggest that the interpretation and uncertainties associated with the gastropod and calcite vein proxies need to be revisited. Used in conjunction with records of carbonate depositional fluxes, our favored seawater Sr/Ca scenarios point to a significant increase in the proportion of aragonite versus calcite deposition in shelf sediments from the Middle Miocene, coincident with the proliferation of coral reefs. We propose that this occurred at least 10 million years after the seawater Mg/Ca threshold was passed, and was instead aided by declining levels of atmospheric carbon dioxide.

  9. Ca2+ signals regulate mitochondrial metabolism by stimulating CREB-mediated expression of the mitochondrial Ca2+ uniporter gene MCU.

    Science.gov (United States)

    Shanmughapriya, Santhanam; Rajan, Sudarsan; Hoffman, Nicholas E; Zhang, Xueqian; Guo, Shuchi; Kolesar, Jill E; Hines, Kevin J; Ragheb, Jonathan; Jog, Neelakshi R; Caricchio, Roberto; Baba, Yoshihiro; Zhou, Yandong; Kaufman, Brett A; Cheung, Joseph Y; Kurosaki, Tomohiro; Gill, Donald L; Madesh, Muniswamy

    2015-03-03

    Cytosolic Ca2+ signals, generated through the coordinated translocation of Ca2+ across the plasma membrane (PM) and endoplasmic reticulum (ER) membrane, mediate diverse cellular responses. Mitochondrial Ca2+ is important for mitochondrial function, and when cytosolic Ca2+ concentration becomes too high, mitochondria function as cellular Ca2+ sinks. By measuring mitochondrial Ca2+ currents, we found that mitochondrial Ca2+ uptake was reduced in chicken DT40 B lymphocytes lacking either the ER-localized inositol trisphosphate receptor (IP3R), which releases Ca2+ from the ER, or Orai1 or STIM1, components of the PM-localized Ca2+ -permeable channel complex that mediates store-operated calcium entry (SOCE) in response to depletion of ER Ca2+ stores. The abundance of MCU, the pore-forming subunit of the mitochondrial Ca2+ uniporter, was reduced in cells deficient in IP3R, STIM1, or Orai1. Chromatin immunoprecipitation and promoter reporter analyses revealed that the Ca2+ -regulated transcription factor CREB (cyclic adenosine monophosphate response element-binding protein) directly bound the MCU promoter and stimulated expression. Lymphocytes deficient in IP3R, STIM1, or Orai1 exhibited altered mitochondrial metabolism, indicating that Ca2+ released from the ER and SOCE-mediated signals modulates mitochondrial function. Thus, our results showed that a transcriptional regulatory circuit involving Ca2+ -dependent activation of CREB controls the Ca2+ uptake capability of mitochondria and hence regulates mitochondrial metabolism.

  10. Feedforward inhibition underlies the propagation of cholinergically induced gamma oscillations from hippocampal CA3 to CA1.

    Science.gov (United States)

    Zemankovics, Rita; Veres, Judit M; Oren, Iris; Hájos, Norbert

    2013-07-24

    Gamma frequency (30-80 Hz) oscillations are implicated in memory processing. Such rhythmic activity can be generated intrinsically in the CA3 region of the hippocampus from where it can propagate to the CA1 area. To uncover the synaptic mechanisms underlying the intrahippocampal spread of gamma oscillations, we recorded local field potentials, as well as action potentials and synaptic currents in anatomically identified CA1 and CA3 neurons during carbachol-induced gamma oscillations in mouse hippocampal slices. The firing of the vast majority of CA1 neurons and all CA3 neurons was phase-coupled to the oscillations recorded in the stratum pyramidale of the CA1 region. The predominant synaptic input to CA1 interneurons was excitatory, and their discharge followed the firing of CA3 pyramidal cells at a latency indicative of monosynaptic connections. Correlation analysis of the input-output characteristics of the neurons and local pharmacological block of inhibition both agree with a model in which glutamatergic CA3 input controls the firing of CA1 interneurons, with local pyramidal cell activity having a minimal role. The firing of phase-coupled CA1 pyramidal cells was controlled principally by their inhibitory inputs, which dominated over excitation. Our results indicate that the synchronous firing of CA3 pyramidal cells rhythmically recruits CA1 interneurons and that this feedforward inhibition generates the oscillatory activity in CA1. These findings identify distinct synaptic mechanisms underlying the generation of gamma frequency oscillations in neighboring hippocampal subregions.

  11. Ca2+-binding protein-1 facilitates and forms a postsynaptic complex with Cav1.2 (L-type) Ca2+ channels.

    Science.gov (United States)

    Zhou, Hong; Kim, Seong-Ah; Kirk, Elizabeth A; Tippens, Alyssa L; Sun, Hong; Haeseleer, Françoise; Lee, Amy

    2004-05-12

    Ca2+-binding protein-1 (CaBP1) is a Ca2+-binding protein that is closely related to calmodulin (CaM) and localized in somatodendritic regions of principal neurons throughout the brain, but how CaBP1 participates in postsynaptic Ca2+ signaling is not known. Here, we describe a novel role for CaBP1 in the regulation of Ca2+ influx through Ca(v)1.2 (L-type) Ca2+ channels. CaBP1 interacts directly with the alpha1 subunit of Ca(v)1.2 at sites that also bind CaM. CaBP1 binding to one of these sites, the IQ domain, is Ca2+ dependent and competitive with CaM binding. The physiological significance of this interaction is supported by the association of Ca(v)1.2 and CaBP1 in postsynaptic density fractions purified from rat brain. Moreover, in double-label immunofluorescence experiments, CaBP1 and Ca(v)1.2 colocalize in numerous cell bodies and dendrites of neurons, particularly in pyramidal cells in the CA3 region of the hippocampus and in the dorsal cortex. In electrophysiological recordings of cells transfected with Ca(v)1.2, CaBP1 greatly prolonged Ca2+ currents, prevented Ca2+-dependent inactivation, and caused Ca2+-dependent facilitation of currents evoked by step depolarizations and repetitive stimuli. These effects contrast with those of CaM, which promoted strong Ca2+-dependent inactivation of Ca(v)1.2 with these same voltage protocols. Our findings reveal how Ca2+-binding proteins, such as CaM and CaBP1, differentially adjust Ca2+ influx through Ca(v)1.2 channels, which may specify diverse modes of Ca2+ signaling in neurons.

  12. The alterations of Ca2+/calmodulin/CaMKII/CaV1.2 signaling in experimental models of Alzheimer's disease and vascular dementia.

    Science.gov (United States)

    Min, Dongyu; Guo, Feng; Zhu, Shu; Xu, Xiaoxue; Mao, Xiaoyuan; Cao, Yonggang; Lv, Xintong; Gao, Qinghua; Wang, Lei; Chen, Tianbao; Shaw, Chris; Hao, Liying; Cai, Jiqun

    2013-03-22

    The two critical forms of dementia are Alzheimer's disease (AD) and vascular dementia (VD). The alterations of Ca(2+)/calmodulin/CaMKII/CaV1.2 signaling in AD and VD have not been well elucidated. Here we have demonstrated changes in the levels of CaV1.2, calmodulin, p-CaMKII, p-CREB and BDNF proteins by Western blot analysis and the co-localization of p-CaMKII/CaV1.2 by double-labeling immunofluorescence in the hippocampus of APP/PS1 mice and VD gerbils. Additionally, expression of these proteins and intracellular calcium levels were examined in cultured neurons treated with Aβ1-42. The expression of CaV1.2 protein was increased in VD gerbils and in cultured neurons but decreased in APP/PS1 mice; the expression of calmodulin protein was increased in APP/PS1 mice and VD gerbils; levels of p-CaMKII, p-CREB and BDNF proteins were decreased in AD and VD models. The number of neurons in which p-CaMKII and CaV1.2 were co-localized, was decreased in the CA1 and CA3 regions in two models. Intracellular calcium was increased in the cultured neurons treated with Aβ1-42. Collectively, our results suggest that the alterations in CaV1.2, calmodulin, p-CaMKII, p-CREB and BDNF can be reflective of an involvement in the impairment in memory and cognition in AD and VD models.

  13. Ca2+ dialogue between acidic vesicles and ER.

    Science.gov (United States)

    Morgan, Anthony J

    2016-04-15

    Extracellular stimuli evoke the synthesis of intracellular second messengers, several of which couple to the release of Ca(2+)from Ca(2+)-storing organelles via activation of cognate organellar Ca(2+)-channel complexes. The archetype is the inositol 1,4,5-trisphosphate (IP3) and IP3receptor (IP3R) on the endoplasmic reticulum (ER). A less understood, parallel Ca(2+)signalling cascade is that involving the messenger nicotinic acid adenine dinucleotide phosphate (NAADP) that couples to Ca(2+)release from acidic Ca(2+)stores [e.g. endo-lysosomes, secretory vesicles, lysosome-related organelles (LROs)]. NAADP-induced Ca(2+)release absolutely requires organellar TPCs (two-pore channels). This review discusses how ER and acidic Ca(2+)stores physically and functionally interact to generate and shape global and local Ca(2+)signals, with particular emphasis on the two-way dialogue between these two organelles.

  14. Reduction of Ca2+-transporting systems in memory T cells.

    Science.gov (United States)

    Sigova, A A; Dedkova, E N; Zinchenko, V P; Litvinov, I S

    2000-01-01

    Antigen-specific B and T lymphocytes make up the material grounds of immune memory, their main functional distinction from the so-called "naive" cells is due to the rapid and enhanced response to the antigen-pathogen. An essential distinction between the memory and naive T cells is different sensitivity of these two subpopulations of T lymphocytes to Ca2+-ionophores. Comparative analysis of Ca2+ responses of the immune memory T lymphocytes and naive T cells of mouse CBA/J line to the addition of Ca2+-mobilizing agents concanavalin A, thapsigargin, and ionomycin was carried out. These compounds in concentrations increasing [Ca2+]i in naive cells had no effect on [Ca2+]i in memory cells. Thus, the Ca2+ entrance into memory cells was not activated by exhaustion of intracellular resources. Estimation of intracellular resources of Ca2+, mobilized by ionomycin and thapsigargin in Ca2+ free medium has shown the absence in memory T cells of the intracellular Ca2+ pool, which may be one of factors of their resistance to ionophores. Reduction of the system of Ca2+ influx into memory T cells was shown using the SH-reagent thimerosal. Memory T cells appear to be resistant to "Ca2+ -paradox." Their incubation with 0.5 mM EDTA in the presence or absence of Ca2+ -mobilizing compounds followed by addition of 2 mM CaCl2 did not result in induction of Ca2+ influx into these cells.

  15. Generation and behavior characterization of CaMKIIβ knockout mice.

    Directory of Open Access Journals (Sweden)

    Adam D Bachstetter

    Full Text Available The calcium/calmodulin-dependent protein kinase II (CaMKII is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ, with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.

  16. Precise half-life measurements for $^{38}$Ca and $^{39}$Ca

    CERN Document Server

    Blank, B; Demonchy, C-E; Borge, M J G; Matea, I; Munoz, F; Huikari, J; Dominguez-Reyes, R; Plaisir, C; Sturm, S; Canchel, G; Delahaye, P; Audirac, L; Fraile, L M; Serani, L; Lunney, D; Pedroza, J-L; Bey, A; Souin, J; Hui, Tran Trong; Delalee, F; Tengblad, O; Wenander, F

    2010-01-01

    The half-lives of Ca-38 and Ca-39 have been measured at ISOLDE of CERN. The REXTRAP facility was used to prepare ultra-clean samples of radioactive nuclei for precision decay spectroscopy. Ca-38 is one of the T-z = -1, 0(+). 0(+) beta-emitting nuclides used to determine the vector coupling constant of the weak interaction and the V-ud quark-mixing matrix element. The result obtained, T-1/2 = 443.8(19) ms, is four times more precise than the average of previous measurements. For Ca-39, a half-life of T-1/2 = 860.7(10) ms is obtained, a result in agreement with the average value from the literature.

  17. Fabrication aspects of PLA-CaP/PLGA-CaP composites for orthopedic applications: a review.

    Science.gov (United States)

    Zhou, Huan; Lawrence, Joseph G; Bhaduri, Sarit B

    2012-07-01

    For several decades, composites made of polylactic acid-calcium phosphates (PLA-CaP) and polylactic acid-co-glycolic acid-calcium phosphates (PLGA-CaP) have seen widespread uses in orthopedic applications. This paper reviews the fabrication aspects of these composites, following the ubiquitous materials science approach by studying "processing-structure-property" correlations. Various fabrication processes such as microencapsulation, phase separation, electrospinning, supercritical gas foaming, etc., are reviewed, with specific examples of their applications in fabricating these composites. The effect of the incorporation of CaP materials on the mechanical and biological performance of PLA/PLGA is addressed. In addition, this paper describes the state of the art on challenges and innovations concerning CaP dispersion, incorporation of biomolecules/stem cells and long-term degradation of the composites.

  18. Glutamate receptor activation in cultured cerebellar granule cells increases cytosolic free Ca2+ by mobilization of cellular Ca2+ and activation of Ca2+ influx

    DEFF Research Database (Denmark)

    Bouchelouche, P; Belhage, B; Frandsen, A;

    1989-01-01

    The Ca2+ sensitive fluorescent probe, fura-2 has been used to monitor cytosolic free calcium levels in mature primary cultures of cerebellar granule cells during exposure to L-glutamate and other excitatory amino acids: quisqualate (QA) kainate (KA) and N-methyl-D-aspartate (NMDA). Glutamate...... at micromolar concentrations produced a prompt and dose-related increase in the intracellular concentration of free Ca2+, ([Ca2+]i), whereas QA, KA and NMDA had no effect. This increase was also seen in the absence of extracellular Ca2+, suggesting that L-glutamate promotes mobilization of Ca2+ from...

  19. Prenatal morphine exposure reduces pyramidal neurons in CA1, CA2 and CA3 subfields of mice hippocampus

    Directory of Open Access Journals (Sweden)

    Soraya Ghafari

    2014-03-01

    Full Text Available Objective(s:This study was carried out to evaluate the effect of maternal morphine exposure during gestational and lactation period on pyramidal neurons of hippocampus in 18 and 32 day mice offspring. Materials and Methods: Thirty female mice were randomly allocated into cases and controls. In case group, animals received morphinesulfate 10 mg/kg.body weight intraperitoneally during 7 days before mating, gestational period (GD 0-21, 18 and 32 days after delivery in the experimental groups. The control animals received an equivalent volume of normal saline. Cerebrum of six offsprings in each group was removed and stained with cresyl violet and a monoclonal antibody NeuN for immunohistochemical detection of surviving pyramidal neurons. Quantitative computer-assisted morphometric study was done on hippocampus. Results: The number of pyramidal neurons in CA1, CA2 and CA3 in treated groups was significantly reduced in postnatal day 18 and 32 (P18, P32 compared to control groups (P

  20. RNAi knockdown of PIK3CA preferentially inhibits invasion of mutant PIK3CA cells

    Institute of Scientific and Technical Information of China (English)

    Xin-Ke Zhou; Sheng-Song Tang; Gao Yi; Min Hou; Jin-Hui Chen; Bo Yang; Ji-Fang Liu; Zhi-Min He

    2011-01-01

    AIM: To explore the effects of siRNA silencing of PIK3CA on proliferation, migration and invasion of gastric cancer cells and to investigate the underlying mechanisms.METHODS: The mutation of PIK3CA in exons 9 and 20 of gastric cancer cell lines HGC-27, SGC-7901, BGC-823, MGC-803 and MKN-45 was screened by poly-merase chain reaction (PCR) followed by sequencing. BGC-823 cells harboring no mutations in either of the exons, and HGC-27 cells containing PIK3CA mutations were employed in the current study. siRNA targeting PIK3CA was chemically synthesized and was transfect-ed into these two cell lines in vitro. mRNA and protein expression of PIK3CA were detected by real-time PCR and Western blotting, respectively. We also measured phosphorylation of a serine/threonine protein kinase (Akt) using Western blotting. The proliferation, migra-tion and invasion of these cells were examined sepa-rately by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-razolium bromide (MTT), wound healing and Transwell chambers assay.RESULTS: The siRNA directed against PIK3CA effec-tively led to inhibition of both endogenous mRNA and protein expression of PIK3CA, and thus significantly down-regulated phosphorylation of Akt (P < 0.05). Furthermore, simultaneous silencing of PIK3CA result-ed in an obvious reduction in tumor cell proliferation activity, migration and invasion potential (P < 0.01). Intriguing, mutant HGC-27 cells exhibited stronger invasion ability than that shown by wild-type BGC-823 cells. Knockdown of PIK3CA in mutant HGC-27 cells contributed to a reduction in cell invasion to a greater extent than in non-mutant BGC-823 cells.CONCLUSION: siRNA mediated targeting of PIK3CA may specifically knockdown the expression of PIK3CA in gastric cancer cells, providing a potential implication for therapy of gastric cancer.