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Sample records for c5 man-machine interaction

  1. Man-machine interactions 3

    CERN Document Server

    Czachórski, Tadeusz; Kozielski, Stanisław

    2014-01-01

    Man-Machine Interaction is an interdisciplinary field of research that covers many aspects of science focused on a human and machine in conjunction.  Basic goal of the study is to improve and invent new ways of communication between users and computers, and many different subjects are involved to reach the long-term research objective of an intuitive, natural and multimodal way of interaction with machines.  The rapid evolution of the methods by which humans interact with computers is observed nowadays and new approaches allow using computing technologies to support people on the daily basis, making computers more usable and receptive to the user's needs.   This monograph is the third edition in the series and presents important ideas, current trends and innovations in  the man-machine interactions area.  The aim of this book is to introduce not only hardware and software interfacing concepts, but also to give insights into the related theoretical background. Reader is provided with a compilation of high...

  2. Human factors and man-machine-interaction

    International Nuclear Information System (INIS)

    Bohr-Bruckmayr, E.

    1985-01-01

    Definitions of the man-machine-interface concept are given. The importance of ergonomics in planning, construction, start-up and operation of a nuclear power plant is highlighted. A comprehensive task analysis is the basis of man-machine-interaction. Personnel performance, work shaping and security are discussed

  3. Understanding and modelling Man-Machine Interaction

    International Nuclear Information System (INIS)

    Cacciabue, P.C.

    1991-01-01

    This paper gives an overview of the current state of the art in man machine systems interaction studies, focusing on the problems derived from highly automated working environments and the role of humans in the control loop. In particular, it is argued that there is a need for sound approaches to design and analysis of Man-Machine Interaction (MMI), which stem from the contribution of three expertises in interfacing domains, namely engineering, computer science and psychology: engineering for understanding and modelling plants and their material and energy conservation principles; psychology for understanding and modelling humans and their cognitive behaviours; computer science for converting models in sound simulations running in appropriate computer architectures. (author)

  4. Understanding and modelling man-machine interaction

    International Nuclear Information System (INIS)

    Cacciabue, P.C.

    1996-01-01

    This paper gives an overview of the current state of the art in man-machine system interaction studies, focusing on the problems derived from highly automated working environments and the role of humans in the control loop. In particular, it is argued that there is a need for sound approaches to the design and analysis of man-machine interaction (MMI), which stem from the contribution of three expertises in interfacing domains, namely engineering, computer science and psychology: engineering for understanding and modelling plants and their material and energy conservation principles; psychology for understanding and modelling humans an their cognitive behaviours; computer science for converting models in sound simulations running in appropriate computer architectures. (orig.)

  5. Man-machine interaction in Canadian nuclear power plants

    International Nuclear Information System (INIS)

    Olmstead, R.A.

    1994-01-01

    The design of man-machine interaction in the CANDU plants has evolved considerably over several generations of plants, from the first Douglas Point plant through to the next generation of plants represented by new designs like CANDU 3. In the early plants, the control room configuration was based on designers' projections of control interface requirements. With succeeding generations of designs, there has been an evolution towards a more systematic consideration of human strengths and weaknesses, increasing attention to formal requirements definition, and incorporation of a larger base of operational experience. This paper describes the design of the man-machine interaction for third generation CANDU-3 control rooms for improved operator reliability and reduced costs. (author). 13 refs., 3 figs

  6. Advanced man-machine interaction. Fundamentals and implementation

    Energy Technology Data Exchange (ETDEWEB)

    Kraiss, K.F. (ed.) [Aachen Technische Hochschule (Germany). Lehrstuhl fuer Technische Informatik und Computerwissenschaften

    2006-07-01

    Man-machine interaction is the gateway providing access to functions and services, which, due to the ever increasing complexity of smart systems, threatens to become a bottleneck. This book therefore introduces not only advanced interfacing concepts, but also gives insight into the related theoretical background.This refers mainly to the realization of video-based multimodal interaction via gesture, mimics, and speech, but also to interacting with virtual object in virtual environments, cooperating with local or remote robots, and user assistance. While most publications in the field of human factors engineering focus on interface design, this book puts special emphasis on implementation aspects. To this end it is accompanied by software development environments for image processing, classification, and virtual environment implementation. In addition a test data base is included for gestures, head pose, facial expressions, full-body person recognition, and people tracking. These data are used for the examples throughout the book, but are also meant to encourage the reader to start experimentation on his own. Thus the book may serve as a self-contained introduction both for researchers and developers of man-machine interfaces. It may also be used for graduate-level university courses. (orig.)

  7. Survey of methods for integrated sequence analysis with emphasis on man-machine interaction

    Energy Technology Data Exchange (ETDEWEB)

    Kahlbom, U; Holmgren, P [RELCON, Stockholm (Sweden)

    1995-05-01

    This report presents a literature study concerning recently developed monotonic methodologies in the human reliability area. The work was performed by RELCON AB on commission by NKS/RAK-1, subproject 3. The topic of subproject 3 is `Integrated Sequence Analysis with Emphasis on Man-Machine Interaction`. The purpose with the study was to compile recently developed methodologies and to propose some of these methodologies for use in the sequence analysis task. The report describes mainly non-dynamic (monotonic) methodologies. One exception is HITLINE, which is a semi-dynamic method. Reference provides a summary of approaches to dynamic analysis of man-machine-interaction, and explains the differences between monotonic and dynamic methodologies. (au) 21 refs.

  8. Survey of methods for integrated sequence analysis with emphasis on man-machine interaction

    International Nuclear Information System (INIS)

    Kahlbom, U.; Holmgren, P.

    1995-05-01

    This report presents a literature study concerning recently developed monotonic methodologies in the human reliability area. The work was performed by RELCON AB on commission by NKS/RAK-1, subproject 3. The topic of subproject 3 is 'Integrated Sequence Analysis with Emphasis on Man-Machine Interaction'. The purpose with the study was to compile recently developed methodologies and to propose some of these methodologies for use in the sequence analysis task. The report describes mainly non-dynamic (monotonic) methodologies. One exception is HITLINE, which is a semi-dynamic method. Reference provides a summary of approaches to dynamic analysis of man-machine-interaction, and explains the differences between monotonic and dynamic methodologies. (au) 21 refs

  9. The simulation of man-machine interaction in NPPs: the system response analyser project

    International Nuclear Information System (INIS)

    Cacciabue, P.C.

    1990-01-01

    In this paper, the ongoing research at Joint Research Centre-Ispra on the simulation of man-machine interaction is reviewed with reference to the past experience of system modelling and to the advances of the technological world. These require the coalescence of mixed disciplines covering the fields of engineering, psychology and sociology. In particular, the complexity of man-machine systems with respect to safety analysis is depicted. The developments and issues in modelling humans and machines are discussed: the possibility of combining them through the System Response Analyser methodology is presented as a balanced to be applied when the objective is the study of safety of systems during abnormal sequences. The three analytical tools which constitute the body of system response analysis namely a quasi-classical simulation of the actual plant, a cognitive model of the operator activities and a driver model, are described. (author)

  10. Study on intelligent processing system of man-machine interactive garment frame model

    Science.gov (United States)

    Chen, Shuwang; Yin, Xiaowei; Chang, Ruijiang; Pan, Peiyun; Wang, Xuedi; Shi, Shuze; Wei, Zhongqian

    2018-05-01

    A man-machine interactive garment frame model intelligent processing system is studied in this paper. The system consists of several sensor device, voice processing module, mechanical parts and data centralized acquisition devices. The sensor device is used to collect information on the environment changes brought by the body near the clothes frame model, the data collection device is used to collect the information of the environment change induced by the sensor device, voice processing module is used for speech recognition of nonspecific person to achieve human-machine interaction, mechanical moving parts are used to make corresponding mechanical responses to the information processed by data collection device.it is connected with data acquisition device by a means of one-way connection. There is a one-way connection between sensor device and data collection device, two-way connection between data acquisition device and voice processing module. The data collection device is one-way connection with mechanical movement parts. The intelligent processing system can judge whether it needs to interact with the customer, realize the man-machine interaction instead of the current rigid frame model.

  11. Man Machine Systems in Education.

    Science.gov (United States)

    Sall, Malkit S.

    This review of the research literature on the interaction between humans and computers discusses how man machine systems can be utilized effectively in the learning-teaching process, especially in secondary education. Beginning with a definition of man machine systems and comments on the poor quality of much of the computer-based learning material…

  12. Analysis of man-machine interaction for control and display system in main control room of light water reactor

    International Nuclear Information System (INIS)

    Santosa, Kussigit; Supriatna, Piping; Karlina, Itjeu; Widagdo, Suharyo; Darlis; Sudiono, Bambang

    1998-01-01

    One of potential hazard in Nuclear Power Plant is the failure of its operation. The accident or operation failure in the reactor must be concerned event its probability is low. The important thing should be concerned is 'Analysis of Man-Machine Interaction (MMI) for Control and Display System in Main Control Room (MCR) of Nuclear Power Reactor', especially LWR type. Control and Display System in MCR of Reactor is the main part of MMI link process in Reactor MCR work system. Signal from display system showed performance process in reactor, while this signal will be received by operator. This signal will be described through central nerve for making decision what kind must be done. Then the operator manage the next process of reactor operation through control system. So by knowing Analysis of Man-Machine Interaction for Control and Display System in Main Control Room of Power Reactor, we can understand human error probability of the operator in reactor operation

  13. Man-machine supervision

    International Nuclear Information System (INIS)

    Montmain, J.

    2005-01-01

    Today's complexity of systems where man is involved has led to the development of more and more sophisticated information processing systems where decision making has become more and more difficult. The operator task has moved from operation to supervision and the production tool has become indissociable from its numerical instrumentation and control system. The integration of more and more numerous and sophisticated control indicators in the control room does not necessary fulfill the expectations of the operation team. It is preferable to develop cooperative information systems which are real situation understanding aids. The stake is not the automation of operators' cognitive tasks but the supply of a reasoning help. One of the challenges of interactive information systems is the selection, organisation and dynamical display of information. The efficiency of the whole man-machine system depends on the communication interface efficiency. This article presents the principles and specificities of man-machine supervision systems: 1 - principle: operator's role in control room, operator and automation, monitoring and diagnosis, characteristics of useful models for supervision; 2 - qualitative reasoning: origin, trends, evolutions; 3 - causal reasoning: causality, causal graph representation, causal and diagnostic graph; 4 - multi-points of view reasoning: multi flow modeling method, Sagace method; 5 - approximate reasoning: the symbolic numerical interface, the multi-criteria decision; 6 - example of application: supervision in a spent-fuel reprocessing facility. (J.S.)

  14. Man machine interaction for operator information systems : a general purpose display package on PC/AT

    International Nuclear Information System (INIS)

    Chandra, A.K.; Dubey, B.P.; Deshpande, S.V.; Vaidya, U.W.; Khandekar, A.B.

    1991-01-01

    Several operator information systems for nuclear plants have been developed at Reactor Control Division of BARC and these have involved extensive operator interaction to extract the maximum information from the systems. Each of these systems used a different scheme for operator interaction. A composite package has now been developed on PC/AT with EGA/VGA for use with any system to obviate the necessity to develop new software for each project. This permits information to be displayed in various formats viz. trend and history curves, tabular data, bar graphs and core matrix (both for 235 and 500 MWe cores). It also allows data to be printed and plotted using multi colour plotter. This package thus integrates all the features of the earlier systems. It also integrates the operator interaction scheme. It uses window based pull down menus to select parameters to be fed into a particular display format. Within any display format the operator has significant flexibility to modify the selected parameters using context dependent soft keys. The package also allows data to be retrieved in machine readable form. This report describes the various user friendly functions implemented and also the design of the system software. (author). 1 tab., 10 fig., 3 refs

  15. Summary of approaches to dynamic analysis of man-machine interaction

    International Nuclear Information System (INIS)

    Hollnagel, E.

    1995-05-01

    The present report was commissioned as a study under the first phase of the NKS/RAK-1, Sub-project 3. The topic of this sub-project is integrated sequence analysis with emphasis on human-system interaction. The report provides the following: a presentation of the principles of dynamic event analysis (joint system simulation), a short survey and characterisation of the main existing systems, and a recommendation of concepts and techniques in relation to the aims of the NKS/RAK-1 project. It is intended that the recommendations shall be used as part of the further planning of the subproject. The work reported here is an extension of the presentation that was given at the first NKS meeting on August 26, 1994. The work has been carried out by Human Reliability Associates, Ltd., in the period October-November, 1994. (au) 20 refs

  16. Summary of approaches to dynamic analysis of man-machine interaction

    Energy Technology Data Exchange (ETDEWEB)

    Hollnagel, E [Human Reliability Analysis Ltd. (United Kingdom)

    1995-05-01

    The present report was commissioned as a study under the first phase of the NKS/RAK-1, Sub-project 3. The topic of this sub-project is integrated sequence analysis with emphasis on human-system interaction. The report provides the following: a presentation of the principles of dynamic event analysis (joint system simulation), a short survey and characterisation of the main existing systems, and a recommendation of concepts and techniques in relation to the aims of the NKS/RAK-1 project. It is intended that the recommendations shall be used as part of the further planning of the subproject. The work reported here is an extension of the presentation that was given at the first NKS meeting on August 26, 1994. The work has been carried out by Human Reliability Associates, Ltd., in the period October-November, 1994. (au) 20 refs.

  17. Man - Machine Communication

    CERN Document Server

    Petersen, Peter; Nielsen, Henning

    1984-01-01

    This report describes a Man-to-Machine Communication module which together with a STAC can take care of all operator inputs from the touch-screen, tracker balls and mechanical buttons. The MMC module can also contain a G64 card which could be a GPIB driver but many other G64 cards could be used. The soft-ware services the input devices and makes the results accessible from the CAMAC bus. NODAL functions for the Man Machine Communication is implemented in the STAC and in the ICC.

  18. Second expert discussion on the BMU/BfS concept of man-machine interaction in nuclear power plants

    International Nuclear Information System (INIS)

    Gersinska, R.; Hennig, R.; Kociok, B.

    1992-04-01

    The purpose of this meeting was to assess the need for further research on the impact of human factors. As a result, the following topics were defined as future research projects: Technical requirements on the interface man-machine during the operation of nuclear power plants; technical, organizational and personal requirements during service, maintenance and repair in nuclear power plants; technical, organizational and personel requirements for accident management. For the examination of these key subjects detailled performance criteria were set up by the BfS. (orig./DG) [de

  19. Man-machine interface requirements - advanced technology

    Science.gov (United States)

    Remington, R. W.; Wiener, E. L.

    1984-01-01

    Research issues and areas are identified where increased understanding of the human operator and the interaction between the operator and the avionics could lead to improvements in the performance of current and proposed helicopters. Both current and advanced helicopter systems and avionics are considered. Areas critical to man-machine interface requirements include: (1) artificial intelligence; (2) visual displays; (3) voice technology; (4) cockpit integration; and (5) pilot work loads and performance.

  20. Man-machine design integration

    Energy Technology Data Exchange (ETDEWEB)

    Carrera, J.P. [Westinghouse Electric Corp., Monroeville, PA (United States). Nuclear Technology Div.; Haentjens, J. [Westinghouse Electric Corp., Brussels (Belgium). Nuclear Technology Div.

    1995-12-31

    The presentation overviews the bases for Man-Machine Interface (MMI) designs that are part of three other presentations during the same conference: Advanced Alarm Management System, Functional Displays and System for Emergency Procedure Execution Monitoring. The MMD group history, team and goals are summarized to give some context to the core of the MMD philosophy and integration. (10 refs., 5 figs.).

  1. Man-machine interfaces analysis system based on computer simulation

    International Nuclear Information System (INIS)

    Chen Xiaoming; Gao Zuying; Zhou Zhiwei; Zhao Bingquan

    2004-01-01

    The paper depicts a software assessment system, Dynamic Interaction Analysis Support (DIAS), based on computer simulation technology for man-machine interfaces (MMI) of a control room. It employs a computer to simulate the operation procedures of operations on man-machine interfaces in a control room, provides quantified assessment, and at the same time carries out analysis on operational error rate of operators by means of techniques for human error rate prediction. The problems of placing man-machine interfaces in a control room and of arranging instruments can be detected from simulation results. DIAS system can provide good technical supports to the design and improvement of man-machine interfaces of the main control room of a nuclear power plant

  2. Man machine interface based on speech recognition

    International Nuclear Information System (INIS)

    Jorge, Carlos A.F.; Aghina, Mauricio A.C.; Mol, Antonio C.A.; Pereira, Claudio M.N.A.

    2007-01-01

    This work reports the development of a Man Machine Interface based on speech recognition. The system must recognize spoken commands, and execute the desired tasks, without manual interventions of operators. The range of applications goes from the execution of commands in an industrial plant's control room, to navigation and interaction in virtual environments. Results are reported for isolated word recognition, the isolated words corresponding to the spoken commands. For the pre-processing stage, relevant parameters are extracted from the speech signals, using the cepstral analysis technique, that are used for isolated word recognition, and corresponds to the inputs of an artificial neural network, that performs recognition tasks. (author)

  3. The CANDU man-machine interface and simulator training

    International Nuclear Information System (INIS)

    Hinchley, E.M.; Yanofsky, N.

    1982-09-01

    The most significant features of the man-machine interface for CANDU power stations are the extensive use of computer-driven colour graphics displays and the small number of manual controls. The man-machine interface in CANDU stations is designed to present the operator with concise, easy-to-understand information. Future developments in the use of computers in safety shutdown systems, and the use of data highway technologies in plant regulating systems will present special requirements and new opportunities in the application of human factors engineering to the control room. Good man-machine interaction depends on operator training as much as on control room design. In Canada computerized training simulators, which indicate plant response to operator action, are being introducted for operator training. Such simulators support training in normal operation of all plant systems and also in the fault management tasks following malfunctions

  4. An investigative study towards constructing anthropocentric Man-Machine System design evaluation methodology

    International Nuclear Information System (INIS)

    Yoshikawa, H.; Gofuku, A.; Itoh, T.; Sasaki, K.

    1992-01-01

    A methodological investigation has been conducted for evaluating the reliability of man-machine interaction in the total Man-Machine System (MMS) from the view-point of safety maintenance for emergent situations of nuclear power plant. Basic considerations in our study are: (i) what are the MMS design data to be evaluated, (ii) how are those MMS design data should be treated, and (iii) how the introduction effects of various operator support tools can be evaluated. The methods of both qualitative and quantitative MMS design evaluation are summarized in this paper, with the system architecture based on man-machine interaction simulation and the related cognitive human error factor analysis. (author)

  5. Man-machine dialogue design and challenges

    CERN Document Server

    Landragin, Frederic

    2013-01-01

    This book summarizes the main problems posed by the design of a man-machine dialogue system and offers ideas on how to continue along the path towards efficient, realistic and fluid communication between humans and machines. A culmination of ten years of research, it is based on the author's development, investigation and experimentation covering a multitude of fields, including artificial intelligence, automated language processing, man-machine interfaces and notably multimodal or multimedia interfaces. Contents Part 1. Historical and Methodological Landmarks 1. An Assessment of the Evolution

  6. Man-machine communication at Forsmark 3

    International Nuclear Information System (INIS)

    Hultquist, Goeran; Norberg, Soeren

    1984-01-01

    The design of Forsmark 3 began in 1976 and the control room layout and equipment were discussed right from the start. Susequent evolution and events in other nuclear power plants have, however, radically changed the direction of the development of the man-machine functions. (author)

  7. Man machine interface and its implementation

    International Nuclear Information System (INIS)

    Hills, B.G.; Boettcher, D.B.; Reed, R.

    1992-01-01

    Sizewell B is the latest nuclear power station to be constructed in the United Kingdom: its Man-Machine Interfaces are therefore, by definition, the state-of-the-art. This paper discusses the principal Man-Machine Interfaces used in the operation of the station, and the systems that implement them. The Man-Machine Interface facilities discussed are: in the Main Control Room, which is used for normal operation and shutdown of the plant: in the Auxiliary Shutdown Room, which allows shutdown of the reactor if evacuation of the main Control Room is necessary: and in the Technical Support Centre, which is used for remote monitoring of the plant. The Man-Machine Interfaces that are described are parts of a station-wide group of interlinked computer systems called the Data Processing and Control System. This system collects data from the plant and displays it to the operators via discrete devices and on graphical computer displays. It also acquires control inputs from the operators via switches, which are then used to provide remote manual control, modulating control and sequence control. The computer system that handles the plant process data and alarm information displays uses a windowing interface with keyboard and trackerball navigation to allow easy retrieval and viewing of information. It is this system that is the main topic of this paper. (author)

  8. A review of warship man-machine-environment system engineering

    Directory of Open Access Journals (Sweden)

    ZHANG Yumei

    2017-03-01

    Full Text Available Warship Man-Machine-Environment System Engineering (MMESE is an integral part of the overall design, and its design principles were proposed according to safety, efficiency, comfort and pleasure. The typical characteristics of MMESE are summarized. The operating environment is extremely terrible on long voyages. High level collaboration is required due to the complex task system and large manpower demand. Owing to the dense computer interface information, the mental cognitive burden on the crew is heavy. The MMESE technology system is divided into four parts:man-machine coordinated, man-environment coordinated, the evaluation of man-machine-environment characteristics and the ergonomic simulation. Based on the MMESE development venation in this paper, the overseas and domestic research statuses are expounded. Interactive optimization can be realized according to the following aspects:researching the basic human characteristics of the crew, applying this to the warship's overall design, and formulating relevant ergonomic standards and norms. Next, Human System Integration (HSI professional engineering was introduced comprehensively into the marines in order to achieve an optimal system. On this basis, we completed the future development trend analysis. All these studies and results have some reference meaning for guiding the integrated optimization of warships as a whole, downsizing the manpower and improving efficiency.

  9. Recent developments in man-machine systems

    International Nuclear Information System (INIS)

    Johannsen, G.

    1987-01-01

    The field of man-machine systems is introduced with its subareas and a short outline of its history of 45 years. Three current lines of development in university and industrial research are emphasized. Today, the human problem solving activities are experimentally investigated and analytically described more vigorously than the control activities. Further, improved information presentations and decision support are made possible through new technologies of computer graphics and expert systems. At last, work on a general design methodology for man-machine systems is in progress. The aim is to better support human operators of dynamic technological systems as well as designers of graphics for visual display units and of dialogue styles. Thereby, safety and availability of the complete system can be increased. (orig.) [de

  10. Design of man-machine-communication-systems

    International Nuclear Information System (INIS)

    Zimmermann, R.

    1975-04-01

    This paper shows some fundamentals of man-machine-communication and deduces demands and recommendations for the design of communication systems. The main points are the directives for the design of optic display systems with details for visual perception and resolution, luminance and contrast, as well as discernibility and coding of displayed information. The most important rules are recommendations for acoustic information systems, control devices and for design of consoles are also given. (orig.) [de

  11. Man-machine interface for the MFTF

    International Nuclear Information System (INIS)

    Speckert, G.C.

    1979-01-01

    In any complex system, the interesting problems occur at the interface of dissimilar subsystems. Control of the Mirror Fusion Test Facility (MFTF) begins with the US Congress, which controls the dollars, which control the people, who control the nine top-level minicomputers, which control the 65 microprocessors, which control the hardware that controls the physics experiment. There are many interesting boundaries across which control must pass, and the one that this paper addresses is the man-machine one. For the MFTF, the man-machine interface consists of a system of seven control consoles, each allowing one operator to communicate with one minicomputer. These consoles are arranged in a hierarchical manner, and both hardware and software were designed in a top-down fashion. This paper describes the requirements and the design of the console system as a whole, as well as the design and operation of the hardware and software of each console, and examines the possible form of a future man-machine interface

  12. Man-machine interface for the MFTF

    Energy Technology Data Exchange (ETDEWEB)

    Speckert, G.C.

    1979-11-09

    In any complex system, the interesting problems occur at the interface of dissimilar subsystems. Control of the Mirror Fusion Test Facility (MFTF) begins with the US Congress, which controls the dollars, which control the people, who control the nine top-level minicomputers, which control the 65 microprocessors, which control the hardware that controls the physics experiment. There are many interesting boundaries across which control must pass, and the one that this paper addresses is the man-machine one. For the MFTF, the man-machine interface consists of a system of seven control consoles, each allowing one operator to communicate with one minicomputer. These consoles are arranged in a hierarchical manner, and both hardware and software were designed in a top-down fashion. This paper describes the requirements and the design of the console system as a whole, as well as the design and operation of the hardware and software of each console, and examines the possible form of a future man-machine interface.

  13. Human capabilities in space. [man machine interaction

    Science.gov (United States)

    Nicogossian, A. E.

    1984-01-01

    Man's ability to live and perform useful work in space was demonstrated throughout the history of manned space flight. Current planning envisions a multi-functional space station. Man's unique abilities to respond to the unforeseen and to operate at a level of complexity exceeding any reasonable amount of previous planning distinguish him from present day machines. His limitations, however, include his inherent inability to survive without protection, his limited strength, and his propensity to make mistakes when performing repetitive and monotonous tasks. By contrast, an automated system does routine and delicate tasks, exerts force smoothly and precisely, stores, and recalls large amounts of data, and performs deductive reasoning while maintaining a relative insensitivity to the environment. The establishment of a permanent presence of man in space demands that man and machines be appropriately combined in spaceborne systems. To achieve this optimal combination, research is needed in such diverse fields as artificial intelligence, robotics, behavioral psychology, economics, and human factors engineering.

  14. New forms of man-machine interaction

    International Nuclear Information System (INIS)

    Mattsson, S.E.; Elmqvist, H.; Brueck, D.

    1986-01-01

    The new workstation with high performance graphics offer new possibilities for visualizing and giving a more concrete form to important concepts. In this project, some of these possibilities have been investigated by developing and implementing a prototype simulator for dynamical systems. The attention has been focused on the user interface for model development. In automatic control the notion of system is important and its representation is a key issue. The simulator support hierarchical block diagrams to describe the model decomposition and the interconnection structure. At the highest level the user sees a block diagram of annotated boxes connected with lines. The user can scroll, pan and zoom the block diagram continuously in real-time. Zooming controls the amount of information displayed. When zooming in on a block, it changes from an annotated box into a representation showing internal structure with increasing detail. Since the block diagrams can be hierarchical, it is possible to make the description at each level simple and clear. The simulator is called Hibliz which stands for HIerarchial BLock diagrams with Information Zooming. The user creates and edits his block diagrams in a Mackintosh-like fashion. He can also create overview windows which indicated where he is when he pans and zooms. Hibliz also simplified model development by allowing submodels in the form of ordinary differential and algebraic equations rather than assignment statements for derivatives and algebraic variables. (authors)

  15. Conceptual models in man-machine design verification

    International Nuclear Information System (INIS)

    Rasmussen, J.

    1985-01-01

    The need for systematic methods for evaluation of design concepts for new man-machine systems has been rapidly increasing in consequence of the introduction of modern information technology. Direct empirical methods are difficult to apply when functions during rare conditions and support of operator decisions during emergencies are to be evaluated. In this paper, the problems of analytical evaluations based on conceptual models of the man-machine interaction are discussed, and the relations to system design and analytical risk assessment are considered. Finally, a conceptual framework for analytical evaluation is proposed, including several domains of description: 1. The problem space, in the form of a means-end hierarchy; 2. The structure of the decision process; 3. The mental strategies and heuristics used by operators; 4. The levels of cognitive control and the mechanisms related to human errors. Finally, the need for models representing operators' subjective criteria for choosing among available mental strategies and for accepting advice from intelligent interfaces is discussed

  16. Man-machine supervision; Supervision homme-machine

    Energy Technology Data Exchange (ETDEWEB)

    Montmain, J. [CEA Valrho, Dir. de l' Energie Nucleaire (DEN), 30 - Marcoule (France)

    2005-05-01

    Today's complexity of systems where man is involved has led to the development of more and more sophisticated information processing systems where decision making has become more and more difficult. The operator task has moved from operation to supervision and the production tool has become indissociable from its numerical instrumentation and control system. The integration of more and more numerous and sophisticated control indicators in the control room does not necessary fulfill the expectations of the operation team. It is preferable to develop cooperative information systems which are real situation understanding aids. The stake is not the automation of operators' cognitive tasks but the supply of a reasoning help. One of the challenges of interactive information systems is the selection, organisation and dynamical display of information. The efficiency of the whole man-machine system depends on the communication interface efficiency. This article presents the principles and specificities of man-machine supervision systems: 1 - principle: operator's role in control room, operator and automation, monitoring and diagnosis, characteristics of useful models for supervision; 2 - qualitative reasoning: origin, trends, evolutions; 3 - causal reasoning: causality, causal graph representation, causal and diagnostic graph; 4 - multi-points of view reasoning: multi flow modeling method, Sagace method; 5 - approximate reasoning: the symbolic numerical interface, the multi-criteria decision; 6 - example of application: supervision in a spent-fuel reprocessing facility. (J.S.)

  17. The methodology of man-machine systems

    International Nuclear Information System (INIS)

    Hollnagel, E.

    1981-10-01

    This paper provides an elementary discussion of the problems of verification and validation in the context of the empirical evaluation of designs for man-machine systems. After a definition of the basic terms, a breakdown of the major parts of the process of evaluation is given, with the purpose of indicating where problems may occur. This is followed by a discussion of verification and validation, as two distinct concepts. Finally, some of the practical problems of ascertaining validity are discussed. The general conclusion is that rather than rely blindly on a well-established procedure or rule, one should pay attention to the meaningfulness of the aspects which are selected for observation, and the degree of systematism of the methods of observation and analysis. A qualitative approach is thus seen as complementary to a quantitative approach, rather than antithetical to it. (author)

  18. Human friendly man-machine system with advanced media technology

    International Nuclear Information System (INIS)

    Ogino, Takamichi; Sasaki, Kazunori

    1993-01-01

    This paper deals with the methodology to implement the man-machine system (MMS) with enhanced human friendliness for nuclear power plants. The relevant technologies are investigated from the two view points: One is integrated multi-media usage for user-computer interface and the other cognitive engineering for user-task interaction. Promising MMS design methodologies, concepts, and their limitations are discussed. To overcome uncertain factors found in human behaviors or individual differences in performance and preference of operators, a design appproach to natural and flexible man-computer interactive environment is proposed by intergrated use of not only cognitive and psychological knowledge but also advanced media technology. Multi-media operator support system under development is shown as an example to evaluate the effectiveness of the new approach and future advancement is prospected. (orig.)

  19. Some trends in man-machine interface design for industrial process plants

    DEFF Research Database (Denmark)

    Rasmussen, Jens

    1980-01-01

    . In the paper, problems related to interface design, operator training and human reliability are discussed in the light of this technological development, and an integrated approach to system design based on a consistent model or framework describing the man-machine interaction is advocated.The work presented......The demands for an efficient and reliable man-machine inter-face in industrial process plant are increasing due to the steadily growing size and complexity of installations. At the same time, computerized technology offers the possibility of powerful and effective solutions to designers...

  20. Man-machine interface builders at the Advanced Photon Source

    International Nuclear Information System (INIS)

    Anderson, M.D.

    1991-01-01

    Argonne National Laboratory is constructing a 7-GeV Advanced Photon Source for use as a synchrotron radiation source in basic and applied research. The controls and computing environment for this accelerator complex includes graphical operator interfaces to the machine based on Motif, X11, and PHIGS/PEX. Construction and operation of the control system for this accelerator relies upon interactive interface builder and diagram/editor type tools, as well as a run-time environment for the constructed displays which communicate with the physical machine via network connections. This paper discusses our experience with several commercial CUI builders, the inadequacies found in these, motivation for the development of an application- specific builder, and design and implementation strategies employed in the development of our own Man-Machine Interface builder. 5 refs

  1. Man-machine interface versus full automation

    International Nuclear Information System (INIS)

    Hatton, V.

    1984-01-01

    As accelerators grow in size and complexity of operation there is an increasing economical as well as an operational incentive for the controls and operations teams to use computers to help the man-machine interface. At first the computer network replaced the traditional controls racks filled with knobs, buttons and digital displays of voltages and potentiometer readings. The computer system provided the operator with the extension of his hands and eyes. It was quickly found that much more could be achieved. Where previously it was necessary for the human operator to decide the order of the actions to be executed by the computer as a result of a visual indication of malfunctioning of the accelerator, now the operation is becoming more and more under the direct control of the computer system. Expert knowledge is programmed into the system to help the non-specialist make decision and to safeguard the equipment. Machine physics concepts have been incorporated and critical machine parameters can be optimised easily by the physicists or operators without any detailed knowledge of the intervening medium or of the equipment being controlled. As confidence grows and reliability improves, more and more automation can be added. How far can this process of automation replace the skilled operator. Can the accelerators of tomorrow be run like the ever increasing robotic assembly plants of today. How is the role of the operator changing in this new environment

  2. Graphic man-machine interface applied to nuclear reactor designs

    International Nuclear Information System (INIS)

    Pereira, Claudio M.N.A; Mol, Antonio Carlos A.

    1999-01-01

    The Man-Machine Interfaces have been of interest of many researchers in the area of nuclear human factors engineering, principally applied to monitoring systems. The clarity of information provides best adaptation of the men to the machine. This work proposes the development of a Graphic Man-Machine Interface applied to nuclear reactor designs as a tool to optimize them. Here is present a prototype of a graphic man-machine interface for the Hammer code developed for PC under the Windows environment. The results of its application are commented. (author)

  3. Activities of IAEA related to human interface in man-machine system

    International Nuclear Information System (INIS)

    Nishiwaki, Yasushi

    1988-01-01

    The present paper outlines some activities of IAEA related to human interface in man-machine systems. It has been recognized for quite some time that in large and complex man-machine interactive systems human errors can contribute substantially to failures of these systems, and that the improvement in the human interface in man-machine systems is essential for the safety of the plant. Many important surveys have been made in some member countries. These studies and operational experience have shown that it is possible to substantially reduce this adverse impact of human errors in nuclear power plant operations by the application of human factors technology. This technology. This technology includes: (1) selection of people with the requisite skills and knowledge and providing them with job-relevant training, (2) maintenance of the necessary job qualifications for each person in the plant, (3) design of man-machine interfaces which are fully compatible with the capabilities and limitations of the people in the system, and (4) design of job operations, including written materials, to facilitate required quality of human performance. A review is made of education/training, operator support systems, human error data collection, analysis of safety significant events and future activities. (Nogami, K.)

  4. 13th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2014-01-01

    The integrated and advanced science research topic Man-Machine-Environment System Engineering (MMESE) was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science and technology in China!”   MMESE primarily focuses on the relationship between man, machines and the environment, studying the optimum combination of man-machine-environment systems. In this system, “man” refers to people in the workplace (e.g. operators, decision-makers); “ machine” is the general name for any object controlled by man (including tools, machinery, computers, systems and technologies), and “environment” describes the specific working conditions under which man and machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization of Man-Machine-Environment system...

  5. 14th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2015-01-01

    The integrated and advanced science research topic man-machine-environment system engineering (MMESE) was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science and technology in China!”   MMESE primarily focuses on the relationship between man, machines and the environment, studying the optimum combination of man-machine-environment systems. In this system, “man” refers to people in the workplace (e.g. operators, decision-makers); “ machine” is the general name for any object controlled by man (including tools, machinery, computers, systems and technologies), and “environment” describes the specific working conditions under which man and machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization of man-machine-environment system...

  6. Man-machine cooperation in remote handling for fusion plants

    International Nuclear Information System (INIS)

    Leinemann, K.

    1984-01-01

    Man-machine cooperation in remote handling for fusion plants comprises cooperation for design of equipment and planning of procedures using a CAD system, and cooperation during operation of the equipment with computer aided telemanipulation systems (CAT). This concept is presently being implemented for support of slave positioning, camera tracking, and camera alignment in the KfK manipulator test facility. The pilot implementation will be used to test various man-machine interface layouts, and to establish a set of basic buildings blocks for future implementations of advanced remote handling control systems. (author)

  7. Proton storage ring: man/machine interface

    International Nuclear Information System (INIS)

    Lander, R.F.; Clout, P.N.

    1985-01-01

    The human interface of the Proton Storage Ring Control System at Los Alamos is described in some detail, together with the software environment in which operator interaction programs are written. Some examples of operator interaction programs are given

  8. Role of the man-machine interface in accident management strategies

    International Nuclear Information System (INIS)

    Oewre, Fridtjov

    2001-01-01

    First, this paper gives a short general review on important safety issues in the field of man-machine interaction as expressed by important nuclear safety organisations. Then follows a summary discussion on what constitutes a modern Man-Machine Interface (MMI) and what is normally meant with accident management and accident management strategies. Furthermore, the paper focuses on three major issues in the context of accident management. First, the need for reliable information in accidents and how this can be obtained by additional computer technology. Second, the use of procedures is discussed, and basic MMI aspects of computer support for procedure presentation are identified followed by a presentation of a new approach on how to computerise procedures. Third, typical information needs for characteristic end-users in accidents, such as the control room operators, technical support staff and plant emergency teams, is discussed. Some ideas on how to apply virtual reality technology in accident management is also presented

  9. Man-machine systems research at the OECD Halden reactor project

    International Nuclear Information System (INIS)

    Owre, F.; Bjorlo, T.J.; Haugset, K.

    1994-01-01

    The OECD Halden Reactor Project is a jointly financed research programme under the auspices of the OECD - Nuclear Energy Agency with fifteen participating countries. One of the main research topics focuses on man-machine systems. Particular attention is paid to the operator's tasks in the reactor control room environment. The overall objective of the research in this field is to provide a basis for improving today's control rooms through the introduction of computer-based solutions for the effective and safe execution of surveillance and control functions in normal as well as off-normal plant situations. The programme comprises four main activities: the verification and validation of safety critical software systems; man-machine interaction research emphasizing improvements in man-machine interfaces on the basis of human factors studies; computerised operator support systems assisting the operator in fault detection/diagnosis and planning of control actions; and control room development providing a basis for retrofitting of existing control rooms and for the design of advanced concepts

  10. 16th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2016-01-01

    This research topic was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science subject and technology in China!” MMESE primarily focuses on the relationship between Man, Machine and Environment, studying the optimum combination of man-machine-environment systems. In this system, “Man” refers to working people as the subject in the workplace (e.g. operators, decision-makers); “Machine” is the general name for any object controlled by Man (including tools, machinery, computers, systems and technologies), and “Environment” describes the specific working conditions under which Man and Machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization are to ensure "Safety, High efficiency and Economy" of man-machine-environment systems. These...

  11. The ELETTRA man-machine interface

    International Nuclear Information System (INIS)

    Potepan, F.; Surace, G.; Mignacco, M.

    1992-01-01

    ELETTRA is a third generation Synchrotron Light Source under construction in Trieste (Italy), with beam energies between 1.5 and 2 GeV. Two networks connect three layers of computers in a fully distributed architecture. An ergonomic and unified approach in the realization of the human interface for the ELETTRA storage ring has led to the adoption of artificial reality criteria for the definition of the system synoptic representation and user interaction. Users can navigate inside a graphic database of the whole system and interactively edit specific virtual control panels to operate on the controlled equipment. UNIX workstations with extended graphic capabilities as operator consoles are used in the implementation of the PSI (Programmable Synoptic Interface), that was developed on top of X11 and PHIGS standards. (author)

  12. Man-machine communication in reactor control using AI methods

    International Nuclear Information System (INIS)

    Klebau, J.; Lindner, A.; Fiedler, U.

    1987-01-01

    In the last years the interest in process control has expecially focused on problems of man-machine communication. It depends on its great importance to process performance and user acceptance. Advanced computerized operator aids, e.g. in nuclear power plants, are as well as their man-machine interface. In the Central Institute for Nuclear Research in Rossendorf a computerized operator support system for nuclear power plants is designed, which is involved in a decentralized process automation system. A similar but simpler system, the Hierarchical Informational System (HIS) at the Rossendorf Research Reactor, works with a computer controlled man-machine interface, based on menu. In the special case of the disturbance analysis program SAAP-2, which is included in the HIS, the limits of menu techniques are obviously. Therefore it seems to be necessary and with extended hard- and software possible to realize an user controlled natural language interface using Artificial Intelligence (AI) methods. The draft of such a system is described. It should be able to learn during a teaching phase all phrases and their meanings. The system will work on the basis of a self-organizing, associative data structure. It is used to recognize a great amount of words which are used in language analysis. Error recognition and, if possible, correction is done by means of a distance function in the word set. Language analysis should be carried out with a simplified word class controlled functional analysis. With this interface it is supposed to get experience in intelligent man-machine communication to enhance operational safety in future. (author)

  13. How artificial intelligence can help [man-machine interface

    International Nuclear Information System (INIS)

    Elm, W.C.

    1988-01-01

    The operator is ultimately responsible for the safe and economical operation of the plant, and must evaluate the accuracy of any system-recommended action or other output. Decision support systems offer a means to improve the man-machine interface by explicitly supporting operator problem solving, rather than complicating decision-making by the need to request an explanation of the rationale behind an expert system's advice during a high stress situation. (author)

  14. Basic researches for advancement of man-machine systems

    International Nuclear Information System (INIS)

    Yoshikawa, Hidekazu

    1994-01-01

    The historical development of plant instrumentation and control system accompanying the introduction of automation is shown by the example of nuclear power plants. It is explained, and the change in the role of operators in the man-machine system is mentioned. Human errors are the serious problem in various fields, and automation resolves it. But complex systems also caused various disasters due to the relation of men and machines. The problem of human factors in high risk system automation is considered as the heightening of reliability and the reduction of burden on workers by decreasing human participation, and the increase of the risk of large accidents due to the lowering of reliability of human elements and the strengthening of the training of workers. Human model and the framework of human error analysis, the development of the system for man-machine system design and information analysis and evaluation, the significance of physiological index measurement and the perspective of the application, the analysis of the behavior of subjects in the abnormality diagnosis experiment using a plant simulator, and the development to the research on mutual adaptation interface are discussed. In this paper, the problem of human factors in system safety, that technical advancement brings about is examined, and the basic research on the advancement of man-machine systems by the author is reported. (K.I.)

  15. Framework for man-machine interface design evaluation system considering cognitive factor

    International Nuclear Information System (INIS)

    Itoh, Toru; Sasaki, Kazunori; Yoshikawa, Hidekazu; Takahashi, Makoto; Furuta, Tomihiko.

    1994-01-01

    It is necessary to improve human reliability in order to gain a higher reliability of the total plant system taking an account of development of plant automation and improvement of machine reliability. Therefore, the role of the man-machine system will come to be important. Accordingly, the evaluation of the man-machine system design information is desired in order to solve the mismatch problem between plant information presented by the man-machine system and information required by the operator comprehensively. This paper discusses required functions and software framework for the man-machine interface design evaluation system. The man-machine interface design evaluation system has features to extract the potential matters which are inherent on the design information of man-machine system by simulating the operator behavior, the plant system and the man-machine system, considering the operator's cognitive performance and time dependency. (author)

  16. A man-machine interface for simulation purposes

    International Nuclear Information System (INIS)

    Galan, J.M.; Almeida, J.M.; Duque, J.M.

    1990-01-01

    This paper describes a man-machine interface, to link any simulator program to a control panel made up of an array of color graphic screens. This task is performed by a communications program which extracts the data from the simulator program, sorts the data, and sends it, through the network, to graphics programs running in workstations. There, the data is displayed on color graphic screens. A graphics package was specifically designed to assist the user handling of complex circuits, and the formatting and display of data on the screens. (author)

  17. Use of models and mockups in verifying man-machine interfaces

    International Nuclear Information System (INIS)

    Seminara, J.L.

    1985-01-01

    The objective of Human Factors Engineering is to tailor the design of facilities and equipment systems to match the capabilities and limitations of the personnel who will operate and maintain the system. This optimization of the man-machine interface is undertaken to enhance the prospects for safe, reliable, timely, and error-free human performance in meeting system objectives. To ensure the eventual success of a complex man-machine system it is important to systematically and progressively test and verify the adequacy of man-machine interfaces from initial design concepts to system operation. Human factors specialists employ a variety of methods to evaluate the quality of the human-system interface. These methods include: (1) Reviews of two-dimensional drawings using appropriately scaled transparent overlays of personnel spanning the anthropometric range, considering clothing and protective gear encumbrances (2) Use of articulated, scaled, plastic templates or manikins that are overlayed on equipment or facility drawings (3) Development of computerized manikins in computer aided design approaches (4) Use of three-dimensional scale models to better conceptualize work stations, control rooms or maintenance facilities (5) Full or half-scale mockups of system components to evaluate operator/maintainer interfaces (6) Part of full-task dynamic simulation of operator or maintainer tasks and interactive system responses (7) Laboratory and field research to establish human performance capabilities with alternative system design concepts or configurations. Of the design verification methods listed above, this paper will only consider the use of models and mockups in the design process

  18. Upper limb functional electrical stimulation devices and their man-machine interfaces.

    Science.gov (United States)

    Venugopalan, L; Taylor, P N; Cobb, J E; Swain, I D

    2015-01-01

    Functional Electrical Stimulation (FES) is a technique that uses electricity to activate the nerves of a muscle that is paralysed due to hemiplegia, multiple sclerosis, Parkinson's disease or spinal cord injury (SCI). FES has been widely used to restore upper limb functions in people with hemiplegia and C5-C7 tetraplegia and has improved their ability to perform their activities of daily living (ADL). At the time of writing, a detailed literature review of the existing upper limb FES devices and their man-machine interfaces (MMI) showed that only the NESS H200 was commercially available. However, the rigid arm splint doesn't fit everyone and prevents the use of a tenodesis grip. Hence, a robust and versatile upper limb FES device that can be used by a wider group of people is required.

  19. Methods and means for improving the man-machine systems for NPP control

    International Nuclear Information System (INIS)

    Konstantinov, L.V.; Rakitin, I.D.

    1984-01-01

    Consideration is being given to the role of ''human factors'' and the ways of improving the man-machine interaction in NPP control and safety systems (CSS). Simulators and tAaining equipment on the basis of dynamic power unit models used for training and improving skill of NPP operatoAs, as well as for mastering collective actions of personnel under accidental conditions are considered in detail. The most perfect program complexes for fast NPP diagnostics and theiA pealization in the Federal Republic of Germany, Japan, Canada, the USA and other countries are described. A special attention is paid to the means and methods of videoterminal dialogue operator interaction with an object both in normal and extreme situations. It is noted that the problems of the man-machine interaction have become the subject of study only in the end of 70s after analyzing the causes of the Three-Mile-Island accident (USA). Publications dealing with the development of perspective control rooms for NPP were analyzed. It was concluded that radical changes both in equipment and principles of organizing the personnel activity will take place in the nearest future. They will be based on the progress in creating dialogue means and computers of the fourth and fifth generations as well as on engineering and psychological and technical aspects of designing

  20. Adaptive Training and Collective Decision Support Based on Man-Machine Interface

    Science.gov (United States)

    2016-03-02

    Based on Man -machine Interface The views, opinions and/or findings contained in this report are those of the author(s) and should not contrued as an...ADDRESS (ES) U.S. Army Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 adaptive training, EEG, man -machine interface...non peer-reviewed journals: Final Report: Adaptive Training and Collective Decision Support Based on Man -machine Interface Report Title The existence of

  1. Advanced man-machine system for nuclear power plants

    International Nuclear Information System (INIS)

    Masui, Takao; Naito, Norio; Kato, Kanji.

    1990-01-01

    Recent development of artificial intelligence(AI) seems to offer new possibility to strengthen the performance of the operator support system. From this point of view, a national project of Advanced Man-Machine System Development for Nuclear Power Plant (MMS-NPP) has been carried out since 1984 as 8-year project. This project aims at establishing advanced operator support functions which support operators in their knowledge-based behaviors and smoother interface with the system. This paper describes the role of MMS-NPP, the support functions and the main feature of the MMS-NPP detailed design with its focus placed on the realization methods using AI technology of the support functions for BWR and PWR plants. (author)

  2. Automated reasoning in man-machine control systems

    International Nuclear Information System (INIS)

    Stratton, R.C.; Lusk, E.L.

    1983-01-01

    This paper describes a project being undertaken at Argonne National Laboratory to demonstrate the usefulness of automated reasoning techniques in the implementation of a man-machine control system being designed at the EBR-II nuclear power plant. It is shown how automated reasoning influences the choice of optimal roles for both man and machine in the system control process, both for normal and off-normal operation. In addition, the requirements imposed by such a system for a rigorously formal specification of operating states, subsystem states, and transition procedures have a useful impact on the analysis phase. The definitions and rules are discussed for a prototype system which is physically simple yet illustrates some of the complexities inherent in real systems

  3. The Engineering Design of Man-Machine Interface for RTS

    International Nuclear Information System (INIS)

    Yenn, T.-C.

    2002-01-01

    The purpose of this paper is to present the engineering design of the advanced Man-Machine Interface (MMI) of the Integrated system for Radwaste Treatment and Storage (RTS) facility in Institute of Nuclear Energy Research (INER) Taiwan, ROC. To build the RTS, a multi-function radwaste facility with a total storage of about 10,000 drums, is a five-year project starting in 2000 including intermediate activity waste treatment and combustible waste storage. The completed engineering design of the MMI is based on proven technologies and digital control systems, enhancing the radwaste management efficiency and reliability of operator's performance as well as assuring the dose exposure of personnel meeting the regulation standard. Over past few years, INER has accumulated extensive experience in the area of radwaste treatment and storage. Therefore, we are confident that we will complete this project with fulfillment of the requirements of RTS

  4. Complement C5a-C5aR interaction enhances MAPK signaling pathway activities to mediate renal injury in trichloroethylene sensitized BALB/c mice.

    Science.gov (United States)

    Zhang, Jia-xiang; Zha, Wan-sheng; Ye, Liang-ping; Wang, Feng; Wang, Hui; Shen, Tong; Wu, Chang-hao; Zhu, Qi-xing

    2016-02-01

    We have previously shown complement activation as a possible mechanism for trichloroethylene (TCE) sensitization, leading to multi-organ damage including the kidneys. In particular, excessive deposition of C5 and C5b-9-the membrane attack complex, which can generate significant tissue damage, was observed in the kidney tissue after TCE sensitization. The present study tested the hypothesis that anaphylatoxin C5a binding to its receptor C5aR mediates renal injury in TCE-sensitized BALB/c mice. BALB/c mice were sensitized through skin challenge with TCE, with or without pretreatment by the C5aR antagonist W54011. Kidney histopathology and the renal functional test were performed to assess renal injury, and immunohistochemistry and fluorescent labeling were carried out to assess C5a and C5aR expressions. TCE sensitization up-regulated C5a and C5aR expressions in kidney tissue, generated inflammatory infiltration, renal tubule damage, glomerular hypercellularity and impaired renal function. Antagonist pretreatment blocked C5a binding to C5aR and attenuated TCE-induced tissue damage and renal dysfunction. TCE sensitization also caused the deposition of major pro-inflammatory cytokines IL-2, TNF-α and IFN-γ in the kidney tissue (P < 0.05); this was accompanied by increased expression of P-p38, P-ERK and P-JNK proteins (P < 0.05). Pretreatment with the C5aR antagonist attenuated the increase of expression of P-p38, P-ERK and P-JNK proteins (P < 0.05) and also consistently reduced the TCE sensitization-induced increase of IL-2, TNF-α and IFN-γ (P < 0.05). These data identify C5a binding to C5aR, MAP kinase activation, and inflammatory cytokine release as a novel mechanism for complement-mediated renal injury by sensitization with TCE or other environmental chemicals. Copyright © 2015 John Wiley & Sons, Ltd.

  5. Man-machine interface in a submarine command and weapon control system: features and design experience

    Directory of Open Access Journals (Sweden)

    Johan H. Aas

    1989-01-01

    Full Text Available Important man-machine interface (MMI issues concerning a submarine command and weapon control system (CWCS such as crew organization, automation level and decision support are discussed in this paper. Generic submarine CWCS functions and operating conditions are outlined. Detailed, dynamic and real-time prototypes were used to support the MMI design. The prototypes are described and experience with detailed prototyping is discussed. Some of the main interaction principles are summarized and a restricted example of the resulting design is given. Our design experience and current work have been used to outline future perspectives of MMI design in naval CWCSs. The need for both formal and experimental approaches is emphasized.

  6. Man--machine interface issues for space nuclear power systems

    International Nuclear Information System (INIS)

    Nelson, W.R.; Haugset, K.

    1991-01-01

    The deployment of nuclear reactors in space necessitates an entirely new set of guidelines for the design of the man--machine interface (MMI) when compared to earth-based applications such as commerical nuclear power plants. Although the design objectives of earth- and space-based nuclear power systems are the same, that is, to produce electrical power, the differences in the application environments mean that the operator's role will be significantly different for space-based systems. This paper explores the issues associated with establishing the necessary MMI guidelines for space nuclear power systems. The generic human performance requirements for space-based systems are described, and the operator roles that are utilized for the operation of current and advanced earth-based reactors are briefly summarized. The development of a prototype advanced control room, the Integrated Surveillance and Control System (ISACS) at the Organization for Economic Cooperation and Development (OECD) Halden Reactor Project is introduced. Finally, preliminary ideas for the use of the ISACS system as a test bed for establishing MMI guidelines for space nuclear systems are presented

  7. Affective Man-Machine Interface: Unveiling Human Emotions through Biosignals

    Science.gov (United States)

    van den Broek, Egon L.; Lisý, Viliam; Janssen, Joris H.; Westerink, Joyce H. D. M.; Schut, Marleen H.; Tuinenbreijer, Kees

    As is known for centuries, humans exhibit an electrical profile. This profile is altered through various psychological and physiological proce-sses, which can be measured through biosignals; e.g., electromyography (EMG) and electrodermal activity (EDA). These biosignals can reveal our emotions and, as such, can serve as an advanced man-machine interface (MMI) for empathic consumer products. However, such a MMI requires the correct classification of biosignals to emotion classes. This chapter starts with an introduction on biosignals for emotion detection. Next, a state-of-the-art review is presented on automatic emotion classification. Moreover, guidelines are presented for affective MMI. Subsequently, a research is presented that explores the use of EDA and three facial EMG signals to determine neutral, positive, negative, and mixed emotions, using recordings of 21 people. A range of techniques is tested, which resulted in a generic framework for automated emotion classification with up to 61.31% correct classification of the four emotion classes, without the need of personal profiles. Among various other directives for future research, the results emphasize the need for parallel processing of multiple biosignals.

  8. Effect of Bacillus pumilus CCIBP-C5 on Musa-Pseudocercospora fijiensis interaction.

    Science.gov (United States)

    Cruz-Martín, Mileidy; Acosta-Suárez, Mayra; Mena, Eilyn; Roque, Berkis; Pichardo, Tatiana; Alvarado-Capó, Yelenys

    2018-02-01

    The effect of antifungal activity of culture filtrate (CF) of Bacillus pumilus strain CCIBP-C5, an isolate from a phyllosphere of banana ( Musa ) leaves, was determined on Pseudocercospora fijiensis challenged banana plants. The CF was shown to decrease the fungal biomass and induce changes in banana plant. In this sense, at 70 days post inoculation (dpi), a lower infection index as well as a decrease in fungal biomass after 6 dpi was obtained in treated plants with respect to control ones. At the same time, changes in the activities of several enzymes related to plant defense responses, such as phenylalanine ammonia lyase, chitinases, β-1,3-glucanases and peroxidases were observed. These results indicate that B. pumilus CCIBP-C5 has a potential role for biological control of P. fijiensis possibly due to the production of antifungal metabolites.

  9. A new tool for man/machine integration

    International Nuclear Information System (INIS)

    Sommer, W.C.

    1981-01-01

    A popular term within the nuclear power industry today, as a result of TMI, is man/machine interface. It has been determined that greater acknowledgement of this interface is necessary within the industry to integrate the design and operational aspects of a system. What is required is an operational tool that can be used early in the engineering stages of a project and passed on later in time to those who will be responsible to operate that particular system. This paper discusses one such fundamental operations tool that is applied to a process system, its display devices, and its operator actions in a methodical fashion to integrate the machine for man's understanding and proper use. This new tool, referred to as an Operational Schematic, is shown and described. Briefly, it unites, in one location, the important operational display devices with the system process devices. A man can now see the beginning and end of each information and control loop to better understand its function within the system. A method is presented whereby in designing for operability, the schematic is utilized in three phases. The method results in two basic documents, one describes ''what'' is to be operated and the other ''how'' it is to be operated. This integration concept has now considered the hardware spectrum from sensor-to-display and operated the display (on paper) to confirm its operability. Now that the design aspects are complete, the later-in-time operational aspects need to be addressed for the man using the process system. Training personnel in operating and testing the process system is as important as the original design. To accomplish these activities, documents are prepared to instruct personnel how to operate (and test) the system under a variety of circumstances

  10. Participation in the ABWR Man-Machine interface design. Applicability to the Spanish Electrical Sector

    International Nuclear Information System (INIS)

    Rodriguez, C.; Manrique Martin, A.; Nunez, J.

    1997-01-01

    Project coordinated by DTN within the advanced reactor programme. Participation in the design activities for the Advanced Boiling Water Reactor (ABWR) man-machine interface was divided into two phases: Phase I: Preparation of drawings for designing, developing and assessing the advanced control room Phase II: Application of these drawings in design activities Participation in this programme has led to the following possible future applications to the electrical sector: 1. Design and implementation of man-machine interfaces 2. Human factor criteria 3. Assessment of man-machine interfaces 4. Functional specification, computerised operating procedures 5. Computerised alarm prototypes. (Author)

  11. A strategy for man-machine system development in process industries

    International Nuclear Information System (INIS)

    Wirstad, J.

    1986-12-01

    A framework for Man-Machine System design in process industry projects is reported. It is based in the Guidelines for the Design of Man-Machine interfaces which have been generated in cooperation within the European Workshop for Industrial Computer Systems (EWICS). The application of EWICS Guidelines in industrial projects is demonstrated by six User Scenarios, which represent typical projects from different industries, e.g. electrical power generation and distribution, water control, pulp and paper production, oil and gas production. In all these projects Man-Machine System design has been conducted. It is recommended in the report that each Company develops its set of Man-Machine Systems Standard techniques/procedures. At present there are several techniques/procedures available which, for moderate costs, can be adapted to specific Company conditions. A menu of such Man-Machine System techniques/procedures is presented. Means of estimating the costs and benefits of Man-Machine System design are also described. (author)

  12. A basic experimental study on mental workload for human cognitive work at man-machine interface

    International Nuclear Information System (INIS)

    Yoshikawa, Hidekazu; Shimoda, Hiroshi; Wakamori, Osamu; Nagai, Yoshinori

    1995-01-01

    The nature and measurement methods of mental workload (MWL) for human cognitive activity at man-machine interface (MMI) were firstly discussed from the viewpoint of human information process model. Then, a model VDT experiment which simplifies the actual human-computer-interaction situation at MMI, was conducted for several subjects, where two subjects participated in experiment series and tried to solve the same cognitive task in competition. Adopted experimental parameters were (i)different kinds of cognitive task, and (ii)cycle time of information display, to see the influence on MWL characteristics from psycho-physiological viewpoint. A special processing unit for eye camera was developed and used for measuring subjects' eye movement characteristics. Concerning data analysis, total number of display presentation until problem solving (ie., total information needed for problem solving) was assumed as anchoring objective measure for MWL, and the investigations were conducted from two aspects; (i)global interpretation on MWL characteristics seen in the subjects' behavior from viewpoint of human information process model, and (ii)applicability of MWL by means of biocybernetic method. As regards to applicability of biocybernetic method, the nature of MWL characteristics was first divided into two aspects : (i)efficiency of visual information acquisition, and (ii)difficulty of inner cognitive process to solve problem, both in time pressure situation. Then, the data analysis results for eye movement characteristics were correlated to (i), while for heart rate characteristics, (ii). (author)

  13. Integrated digital control and man-machine interface for complex remote handing systems

    International Nuclear Information System (INIS)

    Rowe, J.C.; Spille, R.F.; Zimmermann, S.D.

    1987-01-01

    The Advanced Integrated Maintenance System (AIMS) is part of a continuing effort within the Consolidated Fuel Reprocessing Program at Oak Ridge National Laboratory to develop and extend the capabilities of remote manipulation and maintenance technology. The AIMS is a totally integrated approach to remote handling in hazardous environments. State-of-the-art computer systems connected through a high-speed distributed control system that supports the flexibility and expandability needed for large integrated maintenance applications. A man-Machine Interface provides high-level human interaction through a powerful color graphics menu-controlled operator console. An auxiliary control system handles the real-time processing needs for a variety of support hardware. A pair of dedicated fiber-optic-linked master/slave computer systems control the Advanced Servomanipulator master/slave arms using powerful distributed digital processing methods. The FORTH language was used as a real-time operating and development environment for the entire system, and all of these components are integrated into a control room concept that represents the latest advancements in the development of remote maintenance facilities for hazardous environments

  14. Optimizing man-machine performance of a personnel access restriction security system

    International Nuclear Information System (INIS)

    Banks, W.W.; Moore, J.W.

    1988-01-01

    This paper describes a human engineering design and analysis effort for a major security system upgrade at a DOE facility. This upgrade was accomplished by replacing an obsolete and poorly human engineered security screening both the with a new, user oriented, semiautomated, computer-based access control system. Human factors engineers assisted the designer staff in specifying a security access interface to physically and cognitively accommodate all employees which included handicapped individuals in wheel chairs, and several employees who were severely disabled, both visually and aurally. The new access system was intended to control entry into sensitive exclusion areas by requiring personnel to enter a security screening booth and interact with card reader devices and a-simple-to-operate access control panel system. Extensive man-machine testing with prototype mock-ups was conducted to assess human engineered design features and to illuminate potentially confusing or difficult-to-operated hardware placement, layout, and operation sequencing. These evaluations, along with the prototype mock-ups, provided input which resulted in a prototype which was easy to enter, operate, and understand by end users. This prototype later served as the design basis for the final systems design

  15. Integrated digital control and man-machine interface for complex remote handling systems

    International Nuclear Information System (INIS)

    Rowe, J.C.; Spille, R.F.; Zimmermann, S.D.

    1986-12-01

    The Advanced Integrated Maintenance System (AIMS) is part of a continuing effort within the Consolidated Fuel Reprocessing Program at Oak Ridge National Laboratory to develop and extend the capabilities of remote manipulation and maintenance technology. The AIMS is a totally integrated approach to remote handling in hazardous environments. State-of-the-art computer systems connected through a high-speed communication network provide a real-time distributed control system that supports the flexibility and expandability needed for large integrated maintenance applications. A Man-Machine Interface provides high-level human interaction through a powerful color graphics menu-controlled operator console. An auxiliary control system handles the real-time processing needs for a variety of support hardware. A pair of dedicated fiber-optic-linked master/slave computer system control the Advanced Servomanipulator master/slave arms using powerful distributed digital processing methods. The FORTH language was used as a real-time operating and development environment for the entire system, and all of these components are integrated into a control room concept that represents the latest advancements in the development of remote maintenance facilities for hazardous environments

  16. COSY Control Status. First results with rapid prototyped man-machine interface for accelerator control

    Energy Technology Data Exchange (ETDEWEB)

    Hacker, U [Forschungszentrum Juelich, Postfach 1913, 52425 Juelich (Germany); Haberbosch, C [Forschungszentrum Juelich, Postfach 1913, 52425 Juelich (Germany); Henn, K [Forschungszentrum Juelich, Postfach 1913, 52425 Juelich (Germany); Weinert, A [Forschungszentrum Juelich, Postfach 1913, 52425 Juelich (Germany)

    1994-12-15

    The experience gained with the COSY Control System after a six month commissioning period followed by a six month production period will be presented. The COSY Control System runs approximately 300 VME and VXI target systems using a total of about 1000 CPUs, the systems are driven by the diskless operating environment RT/OS, hosted by eight workcells. Application software is implemented using Object-Orientated programming paradigms. All accelerator components become interface functions as instances of an abstract device model class. Methods defined here present an abstract picture of the accelerator giving immediate access to device states and parameters. Operator interaction is defined by building views and controllers for the model. Higher level functions, such as defining an acceleration cycle, are easily developed and modified with the accelerator connected on-line to the model. In the first year of COSY operation the object based approach for a control system, together with a rapid prototyped man-machine interface has brought to light the potential of new functions such as on-line, real time programming on a running system yielding high programming performance. The advantages of this approach have not been, until now, fully appreciated. ((orig.))

  17. Molecular characterization of the gerbil C5a receptor and identification of a transmembrane domain V amino acid that is crucial for small molecule antagonist interaction.

    Science.gov (United States)

    Waters, Stephen M; Brodbeck, Robbin M; Steflik, Jeremy; Yu, Jianying; Baltazar, Carolyn; Peck, Amy E; Severance, Daniel; Zhang, Lu Yan; Currie, Kevin; Chenard, Bertrand L; Hutchison, Alan J; Maynard, George; Krause, James E

    2005-12-09

    Anaphylatoxin C5a is a potent inflammatory mediator associated with pathogenesis and progression of several inflammation-associated disorders. Small molecule C5a receptor (C5aR) antagonist development is hampered by species-specific receptor biology and the associated inability to use standard rat and mouse in vivo models. Gerbil is one rodent species reportedly responsive to small molecule C5aR antagonists with human C5aR affinity. We report the identification of the gerbil C5aR cDNA using a degenerate primer PCR cloning strategy. The nucleotide sequence revealed an open reading frame encoding a 347-amino acid protein. The cloned receptor (expressed in Sf9 cells) bound recombinant human C5a with nanomolar affinity. Alignment of the gerbil C5aR sequence with those from other species showed that a Trp residue in transmembrane domain V is the only transmembrane domain amino acid unique to small molecule C5aR antagonist-responsive species (i.e. gerbil, human, and non-human primate). Site-directed mutagenesis was used to generate human and mouse C5aRs with a residue exchange of this Trp residue. Mutation of Trp to Leu in human C5aR completely eliminated small molecule antagonist-receptor interaction. In contrast, mutation of Leu to Trp in mouse C5aR enabled small molecule antagonist-receptor interaction. This crucial Trp residue is located deeper within transmembrane domain V than residues reportedly involved in C5a- and cyclic peptide C5a antagonist-receptor interaction, suggesting a novel interaction site(s) for small molecule antagonists. These data provide insight into the basis for small molecule antagonist species selectivity and further define sites critical for C5aR activation and function.

  18. Development of nuclear power plant operator simulator for man-machine interface evaluation

    International Nuclear Information System (INIS)

    Nakagawa, Takashi; Nakatani, Yoshio; Sasaki, Kazunori; Yoshikawa, Hidekazu; Takahashi, Makoto; Furuta, Tomihiko; Hasegawa, Akira.

    1997-01-01

    The operational safety in nuclear power plants depends strongly on man machine interfaces (MMI), such as assignment of equipment on control boards and operation procedures in emergency situations. Therefore, the evaluation and analysis methods for the MMI are important. In order for the methods to be practical, the methods should be executed in each step of design and be easy for designers to use. We aim to develop SEAMAID system: a computer supported system for evaluating and analyzing the MMI by simulating the interaction between the operator and the machine. In this paper, we discuss problems of the conventional methods and the required functions of the operator simulator for the SEAMAID. The operator simulator executes not human errors but correct behavior which follows the operational procedure. The SEAMAID evaluates the MMI by finding potential human errors which could occur in the simulated interactions and points out the problematic interaction parts which could induce human errors. We construct the operator simulator by combining the human model which was proposed by Prof. Reason, and the knowledge base model based on the Petri net model. This simulator can treat frequency parameter which represents the degree of frequency of using a certain knowledge. We conduct two sample simulations in different frequency parameters in the same scenario. These simulation results show that even if the operator behaves correctly following the procedure, there are alternative task sequences. We verify that the simulated interactions are in good agreement with the actual one. Also, we propose the method to point out the problematic parts in the interactions based on the working memory consumption. (author)

  19. Development of nuclear power plant operator simulator for man-machine interface evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, Takashi; Nakatani, Yoshio; Sasaki, Kazunori [Mitsubishi Electric Corp., Amagasaki, Hyogo (Japan). Industrial Electronics and Systems Development Lab.; Yoshikawa, Hidekazu; Takahashi, Makoto; Furuta, Tomihiko; Hasegawa, Akira

    1997-08-01

    The operational safety in nuclear power plants depends strongly on man machine interfaces (MMI), such as assignment of equipment on control boards and operation procedures in emergency situations. Therefore, the evaluation and analysis methods for the MMI are important. In order for the methods to be practical, the methods should be executed in each step of design and be easy for designers to use. We aim to develop SEAMAID system: a computer supported system for evaluating and analyzing the MMI by simulating the interaction between the operator and the machine. In this paper, we discuss problems of the conventional methods and the required functions of the operator simulator for the SEAMAID. The operator simulator executes not human errors but correct behavior which follows the operational procedure. The SEAMAID evaluates the MMI by finding potential human errors which could occur in the simulated interactions and points out the problematic interaction parts which could induce human errors. We construct the operator simulator by combining the human model which was proposed by Prof. Reason, and the knowledge base model based on the Petri net model. This simulator can treat frequency parameter which represents the degree of frequency of using a certain knowledge. We conduct two sample simulations in different frequency parameters in the same scenario. These simulation results show that even if the operator behaves correctly following the procedure, there are alternative task sequences. We verify that the simulated interactions are in good agreement with the actual one. Also, we propose the method to point out the problematic parts in the interactions based on the working memory consumption. (author)

  20. Control rooms and man-machine interface in nuclear power plants

    International Nuclear Information System (INIS)

    1990-08-01

    The importance of man-machine interface for ensuring safe and reliable operation of nuclear power plants has always been recognized. Since the early 1970's, the concepts of operator support and human factors have been increasingly used to better define the role of control rooms. In the late 1970's, the lessons learned from experience considerably accelerated the development of recommendations and regulatory requirements governing the resources and data available to operators in nuclear power plant control rooms, and specified the expertise required to assist them in case of need. This document summarizes the steps which have been taken and are being planned around the world to improve the man-machine interface for safe and economic power generation. It intends to present to the reader useful examples on some selected control room design and man-machine interface practices for operation and surveillance of nuclear power plants. 53 refs, 94 figs, 27 tabs

  1. Survey of the problems posed by the man-machine interface, as seen from the angle of facility operators

    International Nuclear Information System (INIS)

    Heinbuch, R.

    1995-01-01

    The man-machine interface in nuclear power plants is an area very much influenced by the vigorous progress in computer technology. The paper describes the causes underlying the innovative power in this field and its impacts on the man-machine interface in nuclear power plants. The benefits brought by the advanced computer systems in the design of the man-machine interface as well as the problems posed through application in practice to safety-relevant plant systems are discussed, and examples are given showing the experience accumulated so far, and the significant changes effected in the man-machine interface. (orig.) [de

  2. Consolidated fuel-reprocessing program:: man/machine interface development for the REMOTEX concept

    International Nuclear Information System (INIS)

    Garin, J.; Clarke, M.M.

    1981-01-01

    This paper describes ongoing research at ORNL to develop a man/machine interface system that can be used to remotely control a system composed of a transporter base and a force-reflecting, servo-controlled manipulator. A unique feature of the concept is the incorporation of totally remote operation. Thus, a major objective is the requirement that an operator have a sense of presence in the remote environment. Man/machine interface requirements for this totally remote operation remain to be developed. Therefore, a simulator is being built to optimize such requirements and the developments are discussed

  3. Comparison of Models Needed for Conceptual Design of Man-Machine Systems in Different Application Domains

    DEFF Research Database (Denmark)

    Rasmussen, Jens

    1986-01-01

    and subjective preferences. For design of man-machine systems in process control, a framework has been developed in terms of separate representation of the problem domain, the decision task, and the information processing strategies required. The author analyzes the application of this framework to a number......For systematic and computer-aided design of man-machine systems, a consistent framework is needed, i. e. , a set of models which allows the selection of system characteristics which serve the individual user not only to satisfy his goal, but also to select mental processes that match his resources...

  4. Aeromechanics and man-machine integration technology opportunities for rotorcraft of the 1990s and beyond

    Science.gov (United States)

    Kerr, Andrew W.

    1989-01-01

    Programs related to rotorcraft aeromechanics and man-machine integration are discussed which will support advanced army rotorcraft design. In aeromechanics, recent advances in computational fluid dynamics will be used to characterize the complex unsteady flowfields of rotorcraft, and a second-generation comprehensive helicopter analysis system will be used along with models of aerodynamics, engines, and control systems to study the structural dynamics of rotor/body configurations. The man-machine integration program includes the development of advanced cockpit design technology and the evaluation of cockpit and mission equipment concepts in a real-time full-combat environment.

  5. Man-Machine Integrated Design and Analysis System (MIDAS): Functional Overview

    Science.gov (United States)

    Corker, Kevin; Neukom, Christian

    1998-01-01

    Included in the series of screen print-outs illustrates the structure and function of the Man-Machine Integrated Design and Analysis System (MIDAS). Views into the use of the system and editors are featured. The use-case in this set of graphs includes the development of a simulation scenario.

  6. A modern approach for the realisation of the man-machine information system

    International Nuclear Information System (INIS)

    Martin, D.; Grensemann, D.

    1980-01-01

    The tool for man-machine communication has been given the name VISCOMP-VISual COmmunication Man-Process. Dependent on the project implementation phase VISCOMP functions either autonomously (planning and definition phases) or in conjunction with a data acquisition system during the operational phase on the plant. The relationship of the required components to the project phases is illustrated. (orig./HP)

  7. Model of large scale man-machine systems with an application to vessel traffic control

    NARCIS (Netherlands)

    Wewerinke, P.H.; van der Ent, W.I.; ten Hove, D.

    1989-01-01

    Mathematical models are discussed to deal with complex large-scale man-machine systems such as vessel (air, road) traffic and process control systems. Only interrelationships between subsystems are assumed. Each subsystem is controlled by a corresponding human operator (HO). Because of the

  8. Reliability Evaluation and Improvement Approach of Chemical Production Man - Machine - Environment System

    Science.gov (United States)

    Miao, Yongchun; Kang, Rongxue; Chen, Xuefeng

    2017-12-01

    In recent years, with the gradual extension of reliability research, the study of production system reliability has become the hot topic in various industries. Man-machine-environment system is a complex system composed of human factors, machinery equipment and environment. The reliability of individual factor must be analyzed in order to gradually transit to the research of three-factor reliability. Meanwhile, the dynamic relationship among man-machine-environment should be considered to establish an effective blurry evaluation mechanism to truly and effectively analyze the reliability of such systems. In this paper, based on the system engineering, fuzzy theory, reliability theory, human error, environmental impact and machinery equipment failure theory, the reliabilities of human factor, machinery equipment and environment of some chemical production system were studied by the method of fuzzy evaluation. At last, the reliability of man-machine-environment system was calculated to obtain the weighted result, which indicated that the reliability value of this chemical production system was 86.29. Through the given evaluation domain it can be seen that the reliability of man-machine-environment integrated system is in a good status, and the effective measures for further improvement were proposed according to the fuzzy calculation results.

  9. Possibilities and expectations for improved man-machine interface in power system control

    Energy Technology Data Exchange (ETDEWEB)

    Asal, H; Burrow, R K; Lindstrom, K; Mocenigo, M; Schellstede, G; Schaffer, G; Serrani, A

    1992-05-01

    The paper describes the hardware, equipment and functions provided to operators for supervising and controlling HVAC power systems. It analyzes the main elements of the man-machine interface (MMI) with particular attention to the recent possibilities afforded by computer technology and full graphic screens. Alarm management and remote control operation are briefly described.

  10. System-ergonomic optimisation of the man-machine system nuclear power plant

    International Nuclear Information System (INIS)

    Fechner, J.B.

    1981-01-01

    The nuclear power plant is described as a man-machine system, the approach of system ergonomics is explained as well as its integration in the process of manufacture and operation, and BMI projects to introduce this approach in a wider scale are reviewed. (orig./GL) [de

  11. Man/machine interface for a nuclear cask remote handling control station: system design requirements

    International Nuclear Information System (INIS)

    Clarke, M.M.; Kreifeldt, J.G.; Draper, J.V.

    1984-01-01

    Design requirements are presented for a control station of a proposed semi-automated facility for remote handling of nuclear waste casks. Functional and operational man/machine interface: controls, displays, software format, station architecture, and work environment. In addition, some input is given to the design of remote sensing systems in the cask handling areas. 18 references, 9 figures, 12 tables

  12. A friend man-machine interface for thermo-hydraulic simulation codes of nuclear installations

    International Nuclear Information System (INIS)

    Araujo Filho, F. de; Belchior Junior, A.; Barroso, A.C.O.; Gebrim, A.

    1994-01-01

    This work presents the development of a Man-Machine Interface to the TRAC-PF1 code, a computer program to perform best estimate analysis of transients and accidents at nuclear power plants. The results were considered satisfactory and a considerable productivity gain was achieved in the activity of preparing and analyzing simulations. (author)

  13. The APS intranet as a man-machine interface

    International Nuclear Information System (INIS)

    Ciarlette, D.; Gerig, R.; McDowell, W.

    1997-01-01

    The Advanced Photon Source at Argonne National Laboratory has implemented a number of methods for people to interact with the accelerator systems. The accelerator operators use Sun workstations running MEDM and WCL to interface interactively with the accelerator, however, many people need to view information rather than interact with the machine. One of the most common interfaces for viewing information at the Advanced Photon Source is the World Wide Web. Information such as operations logbook entries, machine status updates, and displays of archived and current data are easily available to APS personnel. This interface between people and the accelerator has proven to be quite useful. Because the Intranet is operating-system independent and inherently unidirectional, ensuring the prevention of unauthorized or accidental control of the accelerators is straightforward

  14. Attempt on construction of human friendly man-machine interface. Study and apply about human communication; Human friendly na man machine interface kochiku no kokoromi. Ningen no communication no kento to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Tatsuno, J. [Tokyo University of Agriculture, Tokyo (Japan); Kokubo, Y.; Matsumura, I.; Kobayashi, H. [Hosei University, Tokyo (Japan)

    1998-04-01

    This paper describes an attempt on a construction way of human friendly man-machine interface. At first, we do a simple experiment to find out the characteristic of human verbal communication. From the experimental results, we get some rules in case in human verbal communication. We construct the man-machine interface which is based on these rules. Through teaching process, we examine our verbal communication interface comparing with conventional interfaces. From this comparison, we recognize that the verbal communication interface is valid to construct the user-friendly man-machine interface. 12 refs., 9 figs., 2 tabs.

  15. A new workstation based man/machine interface system for the JT-60 Upgrade

    International Nuclear Information System (INIS)

    Yonekawa, I.; Shimono, M.; Totsuka, T.; Yamagishi, K.

    1992-01-01

    Development of a new man/machine interface system was stimulated by the requirements of making the JT-60 operator interface more 'friendly' on the basis of the past five-year operational experience. Eleven Sun/3 workstations and their supervisory mini-computer HIDIC V90/45 are connected through the standard network; Ethernet. The network is also connected to the existing 'ZENKEI' mini-computer system through the shared memory on the HIDIC V90/45 mini-computer. Improved software, such as automatic setting of the discharge conditions, consistency check among the related parameters and easy operation for discharge result data display, offered the 'user-friendly' environments. This new man/machine interface system leads to the efficient operation of the JT-60. (author)

  16. An intelligent man-machine system for future nuclear power plants

    International Nuclear Information System (INIS)

    Takizawa, Yoji; Hattori, Yoshiaki; Itoh, Juichiro; Fukumoto, Akira

    1994-01-01

    The objective of the development of an intelligent man-machine system for future nuclear power plants is enhancement of operational reliability by applying recent advances in cognitive science, artificial intelligence, and computer technologies. To realize this objective, the intelligent man-machine system, aiming to support a knowledge-based decision making process in an operator's supervisory plant control tasks, consists of three main functions, i.e., a cognitive model-based advisor, a robust automatic sequence controller, and an ecological interface. These three functions have been integrated into a console-type nuclear power plant monitoring and control system as a validation test bed. The validation tests in which experienced operator crews participated were carried out in 1991 and 1992. The test results show the usefulness of the support functions and the validity of the system design approach

  17. Love-hate for man-machine metaphors in Soviet physiology: from Pavlov to "physiological cybernetics".

    Science.gov (United States)

    Gerovitch, Slava

    2002-06-01

    This article reinterprets the debate between orthodox followers of the Pavlovian reflex theory and Soviet "cybernetic physiologists" in the 1950s and 60s as a clash of opposing man-machine metaphors. While both sides accused each other of "mechanistic," reductionist methodology, they did not see anything "mechanistic" about their own central metaphors: the telephone switchboard metaphor for nervous activity (the Pavlovians), and the analogies between the human brain and a computer (the cyberneticians). I argue that the scientific utility of machine analogies was closely intertwined with their philosophical and political meanings and that new interpretations of these metaphors emerged as a result of political conflicts and a realignment of forces within the scientific community and in society at large. I suggest that the constant travel of man-machine analogies, back and forth between physiology and technology has blurred the traditional categories of the "mechanistic" and the "organic" in Soviet neurophysiology, as perhaps in the history of physiology in general.

  18. Quantitative evaluation of impedance perception characteristics of humans in the man-machine interface

    International Nuclear Information System (INIS)

    Onish, Keiichi; Kim, Young Woo; Obinata, Goro; Hase, Kazunori

    2013-01-01

    We investigated impedance perception characteristics of humans in the man-machine interface. Sensibility or operational feel about physical properties of machine dynamics is obtained through perception process. We evaluated the impedance perception characteristics of humans who are operating a mechanical system, based on extended Scheffe's subjective evaluation method in full consideration of the influence of impedance level, impedance difference, experiment order, individual difference and so on. Constant method based quantitative evaluation was adopted to investigate the influence of motion frequency and change of the impedance on human impedance perception characteristics. Experimental results indicate that humans perceive impedance of mechanical systems based on comparison process of the dynamical characteristics of the systems. The proposed method can be applied to quantify the design requirement of man-machine interface. The effectiveness of the proposed method is verified through experimental results.

  19. Quantitative evaluation of impedance perception characteristics of humans in the man-machine interface

    Energy Technology Data Exchange (ETDEWEB)

    Onish, Keiichi [Yamaha Motor Co., Shizuoka (Japan); Kim, Young Woo [Daegu Techno Park R and D Center, Seoul (Korea, Republic of); Obinata, Goro [Nagoya University, Nagoya (Japan); Hase, Kazunori [Tokyo Metropolitan University, Tokyo (Japan)

    2013-05-15

    We investigated impedance perception characteristics of humans in the man-machine interface. Sensibility or operational feel about physical properties of machine dynamics is obtained through perception process. We evaluated the impedance perception characteristics of humans who are operating a mechanical system, based on extended Scheffe's subjective evaluation method in full consideration of the influence of impedance level, impedance difference, experiment order, individual difference and so on. Constant method based quantitative evaluation was adopted to investigate the influence of motion frequency and change of the impedance on human impedance perception characteristics. Experimental results indicate that humans perceive impedance of mechanical systems based on comparison process of the dynamical characteristics of the systems. The proposed method can be applied to quantify the design requirement of man-machine interface. The effectiveness of the proposed method is verified through experimental results.

  20. Quantification of human error and common-mode failures in man-machine systems

    International Nuclear Information System (INIS)

    Lisboa, J.J.

    1988-01-01

    Quantification of human performance, particularly the determination of human error, is essential for realistic assessment of overall system performance of man-machine systems. This paper presents an analysis of human errors in nuclear power plant systems when measured against common-mode failures (CMF). Human errors evaluated are improper testing, inadequate maintenance strategy, and miscalibration. The methodology presented in the paper represents a positive contribution to power plant systems availability by identifying sources of common-mode failure when operational functions are involved. It is also applicable to other complex systems such as chemical plants, aircraft and motor industries; in fact, any large man-created, man-machine system could be included

  1. Toward human-centered man-machine system in nuclear power plants

    International Nuclear Information System (INIS)

    Tanabe, Fumiya

    1993-01-01

    The Japanese LWR power plants are classified into 4 categories, from the viewpoints of the control panel in central control room and the extent of automation. Their characteristics are outlined. The potential weaknesses indwelt in the conventional approaches are discussed; that are the loss of applicability to the unanticipated facts and the loss of morale of the operators. The need for the construction of human-centered man-machine system is emphasized in order to overcome these potential weaknesses. The most important features required for the system are, in short term, to support operators in dificulties, and at the same time, in long term, to assure the acquisition and conservation of the personnels' morale and potential to cope with the problems. The concepts of the 'ecological interface' and 'adaptive aiding' system are introduced as the design concepts for the human-centered man-machine system. (J.P.N.)

  2. Man-machine communication based on the computerized operator support system

    International Nuclear Information System (INIS)

    Sano, Y.; Fukumoto, A.; Seki, E.; Tai, I.; Mori, N.; Tsuchida, M.; Sato, N.

    1985-01-01

    Development of a man-machine communication system in a nuclear power plant has been performed, utilizing the new communication technologies and an advanced diagnosis system. In the course of elaborating the communication concept, selection and rearrangement of communication functions in a control room were made based on the human factors engineering. Guidelines and criteria for information display system and operational equipments were also studied and evaluated. The outline of the communication concept and some evaluation test results are described. (author)

  3. US Liquid Metal Fast Breeder Reactor man-machine interface program

    International Nuclear Information System (INIS)

    Vaurio, J.K.; Change, S.A.

    1982-01-01

    The US LMFBR Man-Machine Interface Program is supportive to and an integral part of the LMFBR Safety Program. This paper describes the goal and objectives of the program, and the necessary research and development efforts with a logical structure for the orderly and timely implementation of the prgoram. Current status and near-term and long-term priority activities are also summarized

  4. Defining man-machine cooperation within complex systems: an ergonomic view of automation

    International Nuclear Information System (INIS)

    Lagrange, V.; Cara, F.

    1997-01-01

    Faced with the question of the optimal automation level in the operations of complex systems, ergonomists offer designers procedures, methods and criteria to take human factors into account. These means have been elaborated in the course of ergonomic interventions in projects at EDF. Based on knowledge of the operators' effective role they attempt to define, among the solutions that are technically possible, those that are desirable from the perspective of the whole man-machine system. (authors)

  5. Software and man-machine interface considerations for a nuclear plant computer replacement and upgrade project

    International Nuclear Information System (INIS)

    Diamond, G.; Robinson, E.

    1984-01-01

    Some of the key software functions and Man-Machine Interface considerations in a computer replacement and upgrade project for a nuclear power plant are described. The project involves the installation of two separate computer systems: an Emergency Response Facilities Computer System (ERFCS) and a Plant Process Computer System (PPCS). These systems employ state-of-the-art computer hardware and software. The ERFCS is a new system intended to provide enhanced functions to meet NRC post-TMI guidelines. The PPCS is intended to replace and upgrade an existing obsolete plant computer system. A general overview of the hardware and software aspects of the replacement and upgrade is presented. The work done to develop the upgraded Man-Machine Interface is described. For the ERFCS, a detailed discussion is presented of the work done to develop logic to evaluate the readiness and performance of safety systems and their supporting functions. The Man-Machine Interface considerations of reporting readiness and performance to the operator are discussed. Finally, the considerations involved in the implementation of this logic in real-time software are discussed.. For the PPCS, a detailed discussion is presented of some new features

  6. MITI project on advanced man-machine system for nuclear power plants

    International Nuclear Information System (INIS)

    Kato, Kanji; Watanabe, Takao; Hayakawa, Hiroyasu; Naito, Norio; Masui, Takao; Ogino, Takamichi.

    1988-01-01

    A computerized operator support system (COSS) against abnormal plant conditions was developed as a five-year project from 1980 to 1984, under the sponsorship of the Ministry of International Trade and Industry. The main purpose of the COSS development was to implement the lessons learned from the Three Mile Island accident. The main nuclear industries in Japan participated in the project. The design concept of the operator support functions and the method to implement it were established, and the prototype systems of the COSS for BWR and PWR plants were developed. After the completion of the COSS development, the above participant group once again joined for the work on an advanced man-machine system for nuclear power plants (MMS-NPP). This eight-year project aims at realizing an advanced operator support system by applying artificial intelligence, especially knowledge engineering, and sophisticated man-machine interface devices. Its main objectives are shown. This system configuration, operating method decision system, man-machine communication system, operation and maintenance support functions and so on are described. (Kako, I.)

  7. Exploitation of new information technologies to improve the man-machine interface in particular in nuclear power plants control rooms (SR 2039/7)

    International Nuclear Information System (INIS)

    Fassmann, W.

    1994-01-01

    The establishment of a uniform computer-based user surface requires a qualification of the employed hard- and software which meets safety demands. This problem is outlined, pointing out those parts of safety instrumentation and control which for the time being have to be conventional in order to ensure sufficient safety precaution. Ergonomic problems of man-machine interaction, which have to be assessed when licensing computerized control rooms or mixed ones, are worked out and a way of solving them is shown. In this regard, observational and operational tasks are considered; activities, however, which are related to the modification of computer software remain unconsidered. (orig./DG) [de

  8. Computerized operator support system with new man-machine interface for BWR power plants

    International Nuclear Information System (INIS)

    Monta, K.; Naito, N.; Sugawara, M.; Sato, N.; Mori, N.; Tai, I.; Fukumoto, A.; Tsuchida, M.

    1984-01-01

    Improvement of the man-machine interface of nuclear power plants is an important contribution to the further enhancement of operational safety. In addition, recent advances in computer technology seem to offer the greatest opportunity to date for achieving improvement in the man-machine interface. The development of a computerized operator support system for BWRs has been undertaken since 1980 with the support of the Japanese Government. The conceptual design of this system is based on the role of the operators. The main functions are standby system management, disturbance analysis and post-trip operational guidance. The objective of the standby system management is to monitor the standby status of the engineered safety feature during normal operation to assure its proper functioning at the onset of emergency situations. The disturbance analysis system detects disturbances in the plant in their early stages and informs the plant operators about, for example, the cause of the disturbances, the plant status and possible propagations. Consequently, operators can take corrective actions to prevent unnecessary plant shutdown. The objective of the post trip operational guide is to support operators in diagnosis and corrective action after a plant trip. Its functions are to monitor the performance of the engineered safety feature, to identify the plant status and to guide the appropriate corrective action to achieve safe plant shutdown. The information from the computerized operator support system is supplied to operators through a colour CRT operator console. The authors have evaluated the performance of various new man-machine interfacing tools and proposed a new operator console design. A prototype system has been developed and verification/validation is proceeding with a BWR plant simulator. (author)

  9. A study on advanced man-machine interface system for autonomous nuclear power plants

    International Nuclear Information System (INIS)

    Matsuoka, Takeshi; Numano, Masayoshi; Fukuto, Junji; Sugasawa, Shinobu; Miyazaki, Keiko; Someya, Minoru; Haraki, Nobuo

    1994-01-01

    A man-machine interface(MMI) system of an autonomous nuclear power plant has an advanced function compared with that of the present nuclear power plants. The MMI has a function model of a plant state, and updates and revises this function model by itself. This paper describes the concept of autonomous nuclear power plants, a plant simulator of an autonomous power plant, a contracted function model of a plant state, three-dimensional color graphic display of a plant state, and an event-tree like expression for plant states. (author)

  10. Human reliability analysis in the man-machine interface design review

    International Nuclear Information System (INIS)

    Kim, I.S.

    2001-01-01

    Advanced, computer-based man-machine interface (MMI) is emerging as part of the new design of nuclear power plants. The impact of advanced MMI on the operator performance, and as a result, on plant safety should be thoroughly evaluated before such technology is actually adopted in the plants. This paper discusses the applicability of human reliability analysis (HRA) to support the design review process. Both the first-generation and the second-generation HRA methods are considered focusing on a couple of promising HRA methods, i.e. ATHEANA and CREAM, with the potential to assist the design review process

  11. Re-Design and Beat Testing of the Man-Machine Integration Design and Analysis System: MIDAS

    Science.gov (United States)

    Shively, R. Jay; Rutkowski, Michael (Technical Monitor)

    1999-01-01

    The Man-machine Design and Analysis System (MIDAS) is a human factors design and analysis system that combines human cognitive models with 3D CAD models and rapid prototyping and simulation techniques. MIDAS allows designers to ask 'what if' types of questions early in concept exploration and development prior to actual hardware development. The system outputs predictions of operator workload, situational awareness and system performance as well as graphical visualization of the cockpit designs interacting with models of the human in a mission scenario. Recently, MIDAS was re-designed to enhance functionality and usability. The goals driving the redesign include more efficient processing, GUI interface, advances in the memory structures, implementation of external vision models and audition. These changes were detailed in an earlier paper. Two Beta test sites with diverse applications have been chosen. One Beta test site is investigating the development of a new airframe and its interaction with the air traffic management system. The second Beta test effort will investigate 3D auditory cueing in conjunction with traditional visual cueing strategies including panel-mounted and heads-up displays. The progress and lessons learned on each of these projects will be discussed.

  12. Cognitive task analysis of nuclear power plant operators for man-machine interface design

    International Nuclear Information System (INIS)

    Itoh, J.I.; Yoshimura, S.; Ohtsuka, T.

    1990-01-01

    This paper aims to ascertain and further develop design guidelines for a man-machine interface compatible with plant operators' problem solving strategies. As the framework for this study, operator's information processing activities were modeled, based on J. Rasmussen's framework for cognitive task analysis. Two experiments were carried out. One was an experiment aimed at gaining an understanding of internal mechanisms involved in mistakes and slips which occurred in operators' responses to incidents and accidents. As a result of fifteen cases of operator performance analysis, sixty one human errors were identified. Further analysis of the errors showed that frequently occurring error mechanisms were absent-mindedness, lack of recognition of patterns in diagnosis and failed procedure formulation due to memory lapses. The other kind of experiment was carried out to identify the envelope of trajectories for the operator's search in the problem space consisting of the two dimensions of means-ends and whole-part relations while dealing with transients. Two cases of experimental sessions were conducted with the thinking-aloud method. From analyses based on verbal protocols, trajectories of operator's search were derived, covering from the whole plant level through the component level in the whole-part dimension and covering from the functional purpose level through the physical form level in the means-ends dimension. The findings obtained from these analyses serve as a basis for developing design guidelines for man-machine interfaces in control rooms of nuclear power plants

  13. A review on progress of man-machine interface system designs for Japanese PWRs

    International Nuclear Information System (INIS)

    Yoshikawa, Hidekazu; Yamamoto, Yoshihiro; Magari, Takayuki.

    1994-01-01

    Historical development of Instrumentation and Control (I and C) system designing for the PWR plants in Kansai Electric Co. Ltd is firstly reviewed with respect to the conventional PWRs in the past, brand-new PWRs (Ohi 3/4 units) and advanced PWRs (APWR) in Japan. The major features of the APWR I and C system design are extensive application of digital computer control technology and advanced man-machine interface in order to enhance safety and reliability of total I and C system and to improve human factors in nuclear power plant operation. Comparative study of the APWR's I and C system design with the EPRI's User Requirement Definitions (URD) resulted in that the current Japanese APWR I and C system design meets generally with the EPRI URD conditions except for those items mainly set by the present national regulatory guidelines. The remaining problems in the current I and C system design are discussed which include the issues on future direction of man-machine interface development. (author)

  14. An information theory based approach for quantitative evaluation of man-machine interface complexity

    International Nuclear Information System (INIS)

    Kang, Hyun Gook

    1999-02-01

    In complex and high-risk work conditions, especially such as in nuclear power plants, human understanding of the plant is highly cognitive and thus largely dependent on the effectiveness of the man-machine interface system. In order to provide more effective and reliable operating conditions for future nuclear power plants, developing more credible and easy to use evaluation methods will afford great help in designing interface systems in a more efficient manner. In this study, in order to analyze the human-machine interactions, I propose the Human-processor Communication(HPC) model which is based on the information flow concept. It identifies the information flow around a human-processor. Information flow has two aspects: appearance and content. Based on the HPC model, I propose two kinds of measures for evaluating a user interface from the viewpoint of these two aspects of information flow. They measure the communicative complexity of each aspect. In this study, for the evaluation of the aspect of appearance, I propose three complexity measures: Operation Complexity, Transition Complexity, and Screen Complexity. Each one of these measures has its own physical meaning. Two experiments carried out in this work support the utility of these measures. The result of the quiz game experiment shows that as the complexity of task context increases, the usage of the interface system becomes more complex. The experimental results of the three example systems(digital view, LDP style view and hierarchy view) show the utility of the proposed complexity measures. In this study, for the evaluation of the aspect of content, I propose the degree of informational coincidence, R (K, P) as a measure for the usefulness of an alarm-processing system. It is designed to perform user-oriented evaluation based on the informational entropy concept. It will be especially useful inearly design phase because designers can estimate the usefulness of an alarm system by short calculations instead

  15. An information theory based approach for quantitative evaluation of man-machine interface complexity

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Hyun Gook

    1999-02-15

    In complex and high-risk work conditions, especially such as in nuclear power plants, human understanding of the plant is highly cognitive and thus largely dependent on the effectiveness of the man-machine interface system. In order to provide more effective and reliable operating conditions for future nuclear power plants, developing more credible and easy to use evaluation methods will afford great help in designing interface systems in a more efficient manner. In this study, in order to analyze the human-machine interactions, I propose the Human-processor Communication(HPC) model which is based on the information flow concept. It identifies the information flow around a human-processor. Information flow has two aspects: appearance and content. Based on the HPC model, I propose two kinds of measures for evaluating a user interface from the viewpoint of these two aspects of information flow. They measure the communicative complexity of each aspect. In this study, for the evaluation of the aspect of appearance, I propose three complexity measures: Operation Complexity, Transition Complexity, and Screen Complexity. Each one of these measures has its own physical meaning. Two experiments carried out in this work support the utility of these measures. The result of the quiz game experiment shows that as the complexity of task context increases, the usage of the interface system becomes more complex. The experimental results of the three example systems(digital view, LDP style view and hierarchy view) show the utility of the proposed complexity measures. In this study, for the evaluation of the aspect of content, I propose the degree of informational coincidence, R (K, P) as a measure for the usefulness of an alarm-processing system. It is designed to perform user-oriented evaluation based on the informational entropy concept. It will be especially useful inearly design phase because designers can estimate the usefulness of an alarm system by short calculations instead

  16. FY1995 distributed control of man-machine cooperative multi agent systems; 1995 nendo ningen kyochogata multi agent kikai system no jiritsu seigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    In the near future, distributed autonomous systems will be practical in many situations, e.g., interactive production systems, hazardous environments, nursing homes, and individual houses. The agents which consist of the distributed system must not give damages to human being and should be working economically. In this project man-machine cooperative multi agent systems are studied in many kind of respects, and basic design technology, basic control technique are developed by establishing fundamental theories and by constructing experimental systems. In this project theoretical and experimental studies are conducted in the following sub-projects: (1) Distributed cooperative control in multi agent type actuation systems (2) Control of non-holonomic systems (3) Man-machine Cooperative systems (4) Robot systems learning human skills (5) Robust force control of constrained systems In each sub-project cooperative nature between machine agent systems and human being, interference between artificial multi agents and environment and new function emergence in coordination of the multi agents and the environment, robust force control against for the environments, control methods for non-holonomic systems, robot systems which can mimic and learn human skills were studied. In each sub-project, some problems were hi-lighted and solutions for the problems have been given based on construction of experimental systems. (NEDO)

  17. Human reliability and the man/machine interface: what do we do after the control room review

    International Nuclear Information System (INIS)

    Folley, J.D. Jr.; Schurman, D.L.

    1983-01-01

    The nuclear industry has focused on only one aspect of the man/machine interface--human factors engineering of control rooms - to enhance nuclear power plant (NPP) safety. There are other factors that profoundly affect the reliability of the operators and maintenance personnel of NPP which, thus, affect NPP safety and availability. This paper discusses the factors of training, selection, job engineering, and work satisfaction, describing the potential effects on reactor safety of these factors at the man/machine interface. The impact of these factors on safety and plant availability is illustrated with examples of results obtained in other fields

  18. Asset Analysis Method for the Cyber Security of Man Machine Interface System

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Sung Kon; Kim, Hun Hee; Shin, Yeong Cheol [Korea Hydro and Nuclear Power, Daejeon (Korea, Republic of)

    2010-10-15

    As digital MMIS (Man Machine Interface System) is applied in Nuclear Power Plant (NPP), cyber security is becoming more and more important. Regulatory guide (KINS/GT-N27) requires that implementation plan for cyber security be prepared in NPP. Regulatory guide recommends the following 4 processes: 1) an asset analysis of MMIS, 2) a vulnerability analysis of MMIS, 3) establishment of countermeasures, and 4) establishment of operational guideline for cyber security. Conventional method for the asset analysis is mainly performed with a table form for each asset. Conventional method requires a lot of efforts due to the duplication of information. This paper presents an asset analysis method using object oriented approach for the NPP

  19. Man-machine considerations in nuclear power plant control room design

    International Nuclear Information System (INIS)

    Tennant, D.V.

    1987-01-01

    Although human factors is a subject that has been around for a number of years, this area of design has only recently become known to the power industry. As power plants have grown in size and complexity, the instrumentation required to control and monitor plant processes has increased tremendously. This has been especially true in nuclear power facilities. Although operators are better trained and qualified, very little consideration has been devoted to man-machine interface and the limitations of human operators. This paper explores the historic aspects and design philosophy associated with nuclear plant control rooms. Current problems and solutions are explored along with the components of a control room review. Finally, a survey of future advances in control room design are offered. This paper is concerned with instrumentation, controls, and displays

  20. Asset Analysis Method for the Cyber Security of Man Machine Interface System

    International Nuclear Information System (INIS)

    Kang, Sung Kon; Kim, Hun Hee; Shin, Yeong Cheol

    2010-01-01

    As digital MMIS (Man Machine Interface System) is applied in Nuclear Power Plant (NPP), cyber security is becoming more and more important. Regulatory guide (KINS/GT-N27) requires that implementation plan for cyber security be prepared in NPP. Regulatory guide recommends the following 4 processes: 1) an asset analysis of MMIS, 2) a vulnerability analysis of MMIS, 3) establishment of countermeasures, and 4) establishment of operational guideline for cyber security. Conventional method for the asset analysis is mainly performed with a table form for each asset. Conventional method requires a lot of efforts due to the duplication of information. This paper presents an asset analysis method using object oriented approach for the NPP

  1. Man-machine interface systems for the Sizewell B Nuclear Power Station

    International Nuclear Information System (INIS)

    Boettcher, D.B.

    2004-01-01

    Sizewell B is the first nuclear power station to be built in the United Kingdom using the Pressurised Water Reactor or PWR system. The design is based on stations operating in the United States, but many changes and new features have been introduced to bring it up to date, and to meet United Kingdom practice and regulatory requirements. The Man-Machine Interfaces (MMIs) in the control rooms have been newly designed from first principles, with special attention paid to human factors and the role of the operators. The instrumentation and control (1 and C) systems which interface the MMIs to the process plant, and automate the operation of the station, use advanced technology to achieve high performance and availability. This paper describes the development of the control rooms and 1 and C systems, explaining the thinking that lay behind the principal decisions. (author)

  2. US/DOE Man-Machine Integration program for liquid metal reactors

    International Nuclear Information System (INIS)

    D'Zmura, A.P.; Seeman, S.E.

    1985-03-01

    The United States Department of Energy (DOE) Man-Machine Integration program was started in 1980 as an addition to the existing Liquid Metal Fast Breeder Reactor safety base technology program. The overall goal of the DOE program is to enhance the operational safety of liquid metal reactors by optimum integration of humans and machines in the overall reactor plant system and by application of the principles of human-factors engineering to the design of equipment, subsystems, facilities, operational aids, procedures and environments. In the four years since its inception the program has concentrated on understanding the control process for Liquid Metal Reactors (LMRs) and on applying advanced computer concepts to this process. This paper describes the products that have been developed in this program, present computer-related programs, and plans for the future

  3. JACOS: AI-based simulation system for man-machine system behavior in NPP

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Kazuo; Yokobayashi, Masao; Tanabe, Fumiya [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Kawase, Katsumi [CSK Corp., Tokyo (Japan); Komiya, Akitoshi [Computer Associated Laboratory, Inc., Hitachinaka, Ibaraki (Japan)

    2001-08-01

    A prototype of a computer simulation system named JACOS (JAERI COgnitive Simulation system) has been developed at JAERI (Japan Atomic Energy Research Institute) to simulate the man-machine system behavior in which both the cognitive behavior of a human operator and the plant behavior affect each other. The objectives of this system development is to provide man-machine system analysts with detailed information on the cognitive process of an operator and the plant behavior affected by operator's actions in accidental situations of a nuclear power plant. The simulation system consists of an operator model and a plant model which are coupled dynamically. The operator model simulates an operator's cognitive behavior in accidental situations based on the decision ladder model of Rasmussen, and is implemented using the AI-techniques of the distributed cooperative inference method with the so-called blackboard architecture. Rule-based behavior is simulated using knowledge representation with If-Then type of rules. Knowledge-based behavior is simulated using knowledge representation with MFM (Multilevel Flow Modeling) and qualitative reasoning method. Cognitive characteristics of attentional narrowing, limitation of short-term memory, and knowledge recalling from long-term memory are also taken into account. The plant model of a 3-loop PWR is also developed using a best estimate thermal-hydraulic analysis code RELAP5/MOD2. This report is prepared as User's Manual for JACOS. The first chapter of this report describes both operator and plant models in detail. The second chapter includes instructive descriptions for program installation, building of a knowledge base for operator model, execution of simulation and analysis of simulation results. The examples of simulation with JACOS are shown in the third chapter. (author)

  4. JACOS: AI-based simulation system for man-machine system behavior in NPP

    International Nuclear Information System (INIS)

    Yoshida, Kazuo; Yokobayashi, Masao; Tanabe, Fumiya; Komiya, Akitoshi

    2001-08-01

    A prototype of a computer simulation system named JACOS (JAERI COgnitive Simulation system) has been developed at JAERI (Japan Atomic Energy Research Institute) to simulate the man-machine system behavior in which both the cognitive behavior of a human operator and the plant behavior affect each other. The objectives of this system development is to provide man-machine system analysts with detailed information on the cognitive process of an operator and the plant behavior affected by operator's actions in accidental situations of a nuclear power plant. The simulation system consists of an operator model and a plant model which are coupled dynamically. The operator model simulates an operator's cognitive behavior in accidental situations based on the decision ladder model of Rasmussen, and is implemented using the AI-techniques of the distributed cooperative inference method with the so-called blackboard architecture. Rule-based behavior is simulated using knowledge representation with If-Then type of rules. Knowledge-based behavior is simulated using knowledge representation with MFM (Multilevel Flow Modeling) and qualitative reasoning method. Cognitive characteristics of attentional narrowing, limitation of short-term memory, and knowledge recalling from long-term memory are also taken into account. The plant model of a 3-loop PWR is also developed using a best estimate thermal-hydraulic analysis code RELAP5/MOD2. This report is prepared as User's Manual for JACOS. The first chapter of this report describes both operator and plant models in detail. The second chapter includes instructive descriptions for program installation, building of a knowledge base for operator model, execution of simulation and analysis of simulation results. The examples of simulation with JACOS are shown in the third chapter. (author)

  5. Development of new plant monitoring and control system with advanced man-machine interfaces NUCAMM-80

    International Nuclear Information System (INIS)

    Sato, Hideyuki; Joge, Toshio; Miyake, Masao; Kishi, Shoichi

    1981-01-01

    BWR type nuclear power stations are the typical plants adopting central monitoring system in view of the size of the scale of system and the prevention of radiation exposure. Central control boards became large as much informations and many operating tools are concentrated on them. Recently, the unit capacity has increased, and the safety has been strengthened, therefore more improvement of the man-machine interface is required concerning the monitoring of plant operation. Hitachi Ltd. developed the central monitoring and control system for nuclear power stations ''NUCAMM-80'', concentrating related fundamental techniques such as the collection of plant informations, the expansion of automatic operation, the ergonomic re-evaluation of the arrangement of panels and subsystems, and the effective use of functional hardwares such as controlling computers and cathode ray tubes, for the purposes of improving the reliability of plant operation and the rate of operation, the reduction of the burden of operators and drastic labor saving. The fundamental policy of the development, the construction of the system, panel layout and the collection of informations, the development of the system for plant automation, the development of plant diagnosis and prevention systems, computer system and the merits of this system are described. (Kako, I.)

  6. Human monitoring and decision-making in man/machine systems

    International Nuclear Information System (INIS)

    Johannsen, G.

    1979-01-01

    Monitoring and decision-making together are very well characterizing the role of the human operator in highly automated systems. In this report, the analysis of human monitoring and decision-making behavior as well as its modeling are described. The goal is to present a survey. 'Classic' and optimal control theoretic monitoring models are dealt with. The relationship between attention allocation and eye movements is discussed. As an example for applications, the evaluation of predictor displays by means of the optimal control model is explained. Fault detection in continuous signals and decision-making behavior of the human operator in fault diagnosis during different operation and maintenance situations are illustrated. The computer-aided decision-making is considered as a queueing problem. It is shown to what extent computer-aiding may be based on the state of human activity as measured by psychophysiological quantities. Finally, management information systems for different application areas are mentioned. As an appendix, the report includes an English-written paper in which the possibilities of mathematical modeling of human behavior in complex man-machine systems are critically assessed. (orig.) 891 GL/orig. 892 MKO [de

  7. Human factors evaluation of man-machine interface for periodic safety review of nuclear power plants

    International Nuclear Information System (INIS)

    Lee, Yong Hee; Lee, Jung Woon; Park, Jae Chang; Hwang, In Koo; Lee, Hyun Cheol; Jang, Tong Il; Ku, Jin Young; Kim, Soo Jin

    2004-12-01

    This report describes the research results of human factors assessment on the MMI(Man Machine Interface) equipment as part of Periodic Safety Review(PSR) of Nuclear Power Plants(NPPs). As MMI is a key factor among human factors to be reviewed in PSR, we reviewed the MMI components of nuclear power plants in aspect of human factors engineering. The availability, suitability, and effectiveness of the MMI devices were chosen to be reviewed. The MMI devices were investigated through the review of design documents related to the MMI, survey of control panels, evaluation of experts, and experimental assessment. Checklists were used to perform this assessment and record the review results. The items mentioned by the expert comments to review in detail in relation with task procedures were tested by experiments with operators' participation. For some questionable issues arisen during this MMI review, operator workload and possibility of errors in operator actions were analysed. The reviewed MMI devices contain MCR(Main Control Room), SPDS(Safety Parameter Display System), RSP(Remote Shutdown Panel), and the selected LCBs(Local Control Boards) importantly related to safety. As results of the assessments, any significant problem challenging the safety was not found on human factors in the MMI devices. However, several small items to be changed and improved in suitability of MMI devices were discovered. An action plan is recommended to accommodate the suggestions and review comments. It will enhance the plant safety on MMI area

  8. Study on HRA-based method for assessing digital man-machine interface

    International Nuclear Information System (INIS)

    Li Pengcheng; Dai Licao; Zhang Li; Zhao Ming; Hu Hong

    2014-01-01

    In order to identify the design flaws of digital man-machine interface (MMI) that may trigger human errors or weaken the performance of operators, a HRA-based method (namely HCR + CREAM + HEC) for assessing digital MMI was established. Firstly, the HCR method was used to identify the risk scenarios of high human error probability from the overall event as a whole perspective. Then, for the identified high-risk scenarios, the CREAM was adopted to determine the various error modes and its error probability, and the failure probability was ranked. Finally, the human factors engineering checklist of digital MMI was established according to the characteristics of digital MMI, it was used to check the digital MMI with high error probability in order to identify the design flaws of digital MMI, and the suggestions of optimization were provided. The results show that the provided assessment method can quickly and efficiently identify the design flaws of digital MMI which easily trigger human errors, and the safety of operation of the digital control system for nuclear power plants can be enhanced by optimization of design. (authors)

  9. Man-machine interface systems and operator training program for ABWR in Japan

    International Nuclear Information System (INIS)

    Kunito, Susumu

    2004-01-01

    The Tokyo Electric Power Company (TEPCO) has developed a new Main Control Room design for the Advanced Boiling Water Reactor (ABWR) to improve man-machine interface. New configuration of panels and enhanced automation are some of the features of the ABWR type Main Control Room design. Various technologies such as Cathode Ray Tubes (CRTs) and Flat Displays (FDs) with touch-sensitive operations are contributed to the development of the ABWR type control room design. This design will be first applied to Kashiwazaki-Kariwa Nuclear Power Station unit 6 (K-6). To train the operators sufficiently, TEPCO reviewed the operator training program. Compared with the conventional training, new training menu will be added and the training of ABWR operators will be started 6 months earlier. An ABWR simulator is under construction and training using this simulator is scheduled to be started in August 1994, which is 18 months before fuel loading of K-6. We are reviewing malfunction modes on the simulator. (author)

  10. Man/machine interface algorithm for advanced delayed-neutron signal characterization system

    International Nuclear Information System (INIS)

    Gross, K.C.

    1985-01-01

    The present failed-element rupture detector (FERD) at Experimental Breeder Reactor II (EBR-II) consists of a single bank of delayed-neutron (DN) detectors at a fixed transit time from the core. Plans are currently under way to upgrade the FERD in 1986 and provide advanced DN signal characterization capability that is embodied in an equivalent-recoil-area (ERA) meter. The new configuration will make available to the operator a wealth of quantitative diagnostic information related to the condition and dynamic evolution of a fuel breach. The diagnostic parameters will include a continuous reading of the ERA value for the breach; the transit time, T/sub tr/, for DN emitters traveling from the core to the FERD; and the isotopic holdup time, T/sub h/, for the source. To enhance the processing, interpretation, and display of these parameters to the reactor operator, a man/machine interface (MMI) algorithm has been developed to run in the background on EBR-II's data acquisition system (DAS). The purpose of this paper is to describe the features and implementation of this newly developed MMI algorithm

  11. Digital-image processing improves man-machine communication at a nuclear reactor

    International Nuclear Information System (INIS)

    Cook, S.A.; Harrington, T.P.; Toffer, H.

    1982-01-01

    The application of digital image processing to improve man-machine communication in a nuclear reactor control room is illustrated. At the Hanford N Reactor, operated by UNC Nuclear Industries for the United States Department of Energy, in Richland, Washington, digital image processing is applied to flow, temperature, and tube power data. Color displays are used to present the data in a clear and concise fashion. Specific examples are used to demonstrate the capabilities and benefits of digital image processing of reactor data. N Reactor flow and power maps for routine reactor operations and for perturbed reactor conditions are displayed. The advantages of difference mapping are demonstrated. Image processing techniques have also been applied to results of analytical reactor models; two examples are shown. The potential of combining experimental and analytical information with digital image processing to produce predictive and adaptive reactor core models is discussed. The applications demonstrate that digital image processing can provide new more effective ways for control room personnel to assess reactor status, to locate problems and explore corrective actions. 10 figures

  12. State of the art in nuclear telerobotics: focus on the man/machine connection

    Science.gov (United States)

    Greaves, Amna E.

    1995-12-01

    The interface between the human controller and remotely operated device is a crux of telerobotic investigation today. This human-to-machine connection is the means by which we communicate our commands to the device, as well as the medium for decision-critical feedback to the operator. The amount of information transferred through the user interface is growing. This can be seen as a direct result of our need to support added complexities, as well as a rapidly expanding domain of applications. A user interface, or UI, is therefore subject to increasing demands to present information in a meaningful manner to the user. Virtual reality, and multi degree-of-freedom input devices lend us the ability to augment the man/machine interface, and handle burgeoning amounts of data in a more intuitive and anthropomorphically correct manner. Along with the aid of 3-D input and output devices, there are several visual tools that can be employed as part of a graphical UI that enhance and accelerate our comprehension of the data being presented. Thus an advanced UI that features these improvements would reduce the amount of fatigue on the teleoperator, increase his level of safety, facilitate learning, augment his control, and potentially reduce task time. This paper investigates the cutting edge concepts and enhancements that lead to the next generation of telerobotic interface systems.

  13. Critical survey of research on human factors and the man-machine interaction

    International Nuclear Information System (INIS)

    Watson, L.A.

    1984-01-01

    A case is developed for placing a high priority on research into human factors in the nuclear power industry. This is based essentially on the fact that human error is a significant factor in plant reliability and the assurance of safety. Control of human error can therefore produce benefits in the reduction of both operational costs and public risk. Descriptions are given of activities initiated by the Commission of the European Communities in conjunction with institutes within the Member States. These include: a comprehensive survey and analysis of current relevant work; considerations of classification schemes for human factors activities; the use of simulators for human factors research; and a proposed European collaborative research programme. (author)

  14. Towards comfortable and efficient man-machine interaction in the cabins of vehicles

    NARCIS (Netherlands)

    Looze, M.P. de; Roetting, M.; Vink, P.; Luczak, H.

    2000-01-01

    The comfort of the operators in the cabins of vehicles is getting an increasingly important issue. At first glance, the cabins of professional machines (earth-moving equipment, busses, harvesting equipment) might look modern and highly comfortable. However, this does not imply that the cabins

  15. Contributions of speech science to the technology of man-machine voice interactions

    Science.gov (United States)

    Lea, Wayne A.

    1977-01-01

    Research in speech understanding was reviewed. Plans which include prosodics research, phonological rules for speech understanding systems, and continued interdisciplinary phonetics research are discussed. Improved acoustic phonetic analysis capabilities in speech recognizers are suggested.

  16. Bridge between control science and technology. Volume 5 Manufacturing man-machine systems, computers, components, traffic control, space applications

    Energy Technology Data Exchange (ETDEWEB)

    Rembold, U; Kempf, K G; Towill, D R; Johannsen, G; Paul, M

    1985-01-01

    Among the topics discussed are: robotics; CAD/CAM applications; and man-machine systems. Consideration is also given to: tools and software for system design and integration; communication systems for real-time computer control; fail-safe design of real-time computer systems; and microcomputer-based control systems. Additional topics discussed include: programmable and intelligent components and instruments in automatic control; transportation systems; and space applications of automatic control systems.

  17. Evaluation and optimization of the man-machine interface in nuclear power plants using the HEROS procedure in PSA

    International Nuclear Information System (INIS)

    Richei, A.; Koch, M.K.; Unger, H.; Hauptmanns, U.

    1998-01-01

    For the probabilistic evaluation and optimization of the man-machine-system a new procedure is developed. This and the resulting expert system, which is based on the fuzzy set theory, is called HEROS, an acronym for Human Error Rate Assessment and Optimizing System. There are several existing procedures for the probabilistic evaluation of human errors, which have several disadvantages. However, in all of these procedures fuzzy verbal expressions are often used for the evaluation of human factors, also in the best known procedures. This use of verbal expressions for describing performance-shaping factors, enabling the evaluation of human factors is the basic approach for HEROS. A model of a modem man-machine-system is the basis of the procedure. Results from ergonomic studies are used to establish a rule-based expert system. HEROS simplifies the importance analysis for the evaluation of human factors, which is necessary for the optimization of the man-machine-system. It can be used in all areas of probabilistic safety assessment. The application of HEROS in the scope of accident management procedures and the comparison with the results of other procedures as an example for the usefulness and substantially more extensive applicability of this new procedure will be shown. (author)

  18. Function analysis of nuclear power plants for developing of man-machine interface system for Korean next generation reactor

    Energy Technology Data Exchange (ETDEWEB)

    Goo, In Soo; Kim, Jang Yyul; Kim, Jung Soo; Kim, Chang Hoi; Na, Nan Joo; Park, Keun Ok; Park, Won Man; Park, Jae Chang; Suh, Sang Moon; Oh, In Suk; Lee, Dong Young; Lee, Yong Hee; Cha, Kyung Ho; Chun, Se Woo; Hur, Sup [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of); Jung, Kyung Hoon [Korea Electric Power Co., Seoul (Korea, Republic of); Park, Yeon Sik; Lee, Bum Joo [Korea Power Engineering Company, Inc., Seoul (Korea, Republic of)

    1995-02-01

    In this study, we developed the methodology and implementation plant of function analysis and performed function analysis, which is one of the major activities for the development of Man-Machine Interface System of the KNGR. Identifying the functions of existing plants followed by structuring the functions, we established functions structured at the conceptual and middle levels. This structure was further checked if it would satisfy regulatory requirements and developed to include the aspects of plant performance and other plant features to emphasize its practicality for the application to the design. (Author) 13 refs., 8 figs., 4 tabs.

  19. Evolution of computer-based surveillance, control and man-machine communication systems in nuclear power stations

    International Nuclear Information System (INIS)

    Wilkinson, H.M.

    1980-01-01

    Reactor surveillance systems, man-machine communication, control methods and system relibility have steadily improved with successive designs. These improvements have been dramatic. Operational results from nine power-reactor units and preliminary results from 12 more units in various phases of implementation are now becoming available. The scope of the improvements ranges from the first design which used only lantern type displays to the latest which uses 18 colour cathode ray tubes (CRT). The operator can bnow scan a parallel presentation of annunciator messages, bar charts and graphical presentations. (orig./HP)

  20. Leadership, communication and decision making in man-machine systems; Fuehrung, Kommunikation und Entscheidungsfindung in Mensch-Maschine-Systemen

    Energy Technology Data Exchange (ETDEWEB)

    Bluhm, Thomas

    2008-07-01

    The contribution under consideration spans a wide curve from the first deadly accident of motorized aviation in the year 1908 up to newer tendencies of the so-called team resources management on observation points and control stations in order to pursue fundamental questions in man-machine systems. A continuous differentiation in the consideration of the factor human beings in complex technical systems is described. This is illustrated by the example of concepts for leadership, communication and decision making on observation points and control stations.

  1. Function analysis of nuclear power plants for developing of man-machine interface system for Korean next generation reactor

    International Nuclear Information System (INIS)

    Goo, In Soo; Kim, Jang Yyul; Kim, Jung Soo; Kim, Chang Hoi; Na, Nan Joo; Park, Keun Ok; Park, Won Man; Park, Jae Chang; Suh, Sang Moon; Oh, In Suk; Lee, Dong Young; Lee, Yong Hee; Cha, Kyung Ho; Chun, Se Woo; Hur, Sup; Jung, Kyung Hoon; Park, Yeon Sik; Lee, Bum Joo

    1995-02-01

    In this study, we developed the methodology and implementation plant of function analysis and performed function analysis, which is one of the major activities for the development of Man-Machine Interface System of the KNGR. Identifying the functions of existing plants followed by structuring the functions, we established functions structured at the conceptual and middle levels. This structure was further checked if it would satisfy regulatory requirements and developed to include the aspects of plant performance and other plant features to emphasize its practicality for the application to the design. (Author) 13 refs., 8 figs., 4 tabs

  2. Man-Machine Integration Design and Analysis System (MIDAS) v5: Augmentations, Motivations, and Directions for Aeronautics Applications

    Science.gov (United States)

    Gore, Brian F.

    2011-01-01

    As automation and advanced technologies are introduced into transport systems ranging from the Next Generation Air Transportation System termed NextGen, to the advanced surface transportation systems as exemplified by the Intelligent Transportations Systems, to future systems designed for space exploration, there is an increased need to validly predict how the future systems will be vulnerable to error given the demands imposed by the assistive technologies. One formalized approach to study the impact of assistive technologies on the human operator in a safe and non-obtrusive manner is through the use of human performance models (HPMs). HPMs play an integral role when complex human-system designs are proposed, developed, and tested. One HPM tool termed the Man-machine Integration Design and Analysis System (MIDAS) is a NASA Ames Research Center HPM software tool that has been applied to predict human-system performance in various domains since 1986. MIDAS is a dynamic, integrated HPM and simulation environment that facilitates the design, visualization, and computational evaluation of complex man-machine system concepts in simulated operational environments. The paper will discuss a range of aviation specific applications including an approach used to model human error for NASA s Aviation Safety Program, and what-if analyses to evaluate flight deck technologies for NextGen operations. This chapter will culminate by raising two challenges for the field of predictive HPMs for complex human-system designs that evaluate assistive technologies: that of (1) model transparency and (2) model validation.

  3. Energetics of the interaction of the antitumour agent titanocene dichloride with glycine: enthalpy of formation of [Ti(η5-C5H5)2(OOCCH2NH3)2]Cl2

    International Nuclear Information System (INIS)

    Nunes, Paulo M.; Minas da Piedade, Manuel E.

    2004-01-01

    The standard molar enthalpy of formation of [Ti(η 5 -C 5 H 5 ) 2 (OOCCH 2 NH 3 ) 2 ]Cl 2 in the crystalline state, at T=298.15 K, was determined as Δ f H m 0 {[Ti(η 5 -C 5 H 5 ) 2 (OOCCH 2 NH 3 ) 2 ]Cl 2 , cr}=-(1447.6 ± 8.1) kJ · mol -1 , by reaction-solution calorimetry. A discussion of the energetics of the direct interaction of the antitumour agent Ti(η 5 -C 5 H 5 ) 2 Cl 2 with glycine is also presented

  4. Human factors engineering measures taken by nuclear power plant owners/operators for optimisation of the man-machine interface

    International Nuclear Information System (INIS)

    Eisgruber, H.

    1996-01-01

    Both operating results and human factors studies show that man is able to meet the requirements in this working environment. Hence the degree of human reliability required by the design basis of nuclear power plants is ensured. This means: - Nuclear technology for electricity generation is justifiable from the human factors point of view. - The chief opponent is not right in saying that man is not able to cope with the risks and challenges brought about by nuclear technology applications. The human factors concept for optimisation or configuration of the man-machine systems represents an additional endeavor on the part of nuclear power plant operators within the framework of their responsibilities. Human factors analyses meet with good response by the personnel, as analysis results and clarification of causes of accident scenarios contribute to relieve the personnel (exoneration) and find ways for remedial action. (orig./DG) [de

  5. Web-based Java application to advanced JT-60 Man-Machine Interfacing System for remote experiments

    International Nuclear Information System (INIS)

    Totsuka, Toshiyuki; Suzuki, Yoshio; Sakata, Shinya; Oshima, Takayuki; Iba, Katsuyuki

    2008-01-01

    Since remote participation in ITER experiments is planned, it is expected to demonstrate that the JT-60SA experiment is controlled from a Japanese remote experiment center located in Rokkasho-mura, Aomori-ken, Japan as a part of the ITER-BA project. Functions required for this experiment are monitoring of the discharge sequence status, handling of the discharge parameter, checking of experiment data, and monitoring of plant data, all of which are included in the existing JT-60 Man-Machine Interfacing System (MMIF). The MMIF is now only available to on-site users at the Naka site due to network safety. The motivation for remote MMIF is prompted by the issue of developing and achieving compatibility with network safety. The Java language has been chosen to implement this task. This paper deals with details of the JT-60 MMIF for the remote experiment that has evolved using the Java language

  6. A basic experimental study on characteristics of on-line human information processing associated with man-machine interface

    International Nuclear Information System (INIS)

    Yoshikawa, Hidekazu; Shimoda, Hiroshi; Nagai, Yoshinori; Kojima, Shin-ichi.

    1990-01-01

    Regarding human factors research on man-machine interface, a basic psychological experiment was conducted to observe psycho-physiological characteristics of on-line human cognitive behavior when cognitive tasks on learning and pattern classification were given to subjects by personal computer using a simple state transition model. During the experiment, three different types of subjects' data were recorded: (i) eye movement data by eye mark recorder, (ii) physio-electric signals by polygraph and (iii) verbal reports. Those subjects' data were analyzed with respect to: (i) the related human cognitive characteristics concerning problem solving strategy, measures of problem difficulty and mental image effect, (ii) observed eye movement characteristics such as saccade, attention, pupil reaction and blinking, etc., and (iii) obtained characteristics of skin potential response and heart rate. It was found that the application of psycho-physiological measurement would serve to objective and detailed analysis of on-line cognitive process. (author)

  7. Human Factors Evaluation of Man-Machine Interface for Periodic Safety Review of Yonggwang Unit no. 1, 2

    International Nuclear Information System (INIS)

    Lee, Yong Hee; Lee, Jung Woon; Park, Jae Chang

    2006-01-01

    This report describes the research results of human factors assessment on the MMI(Man Machine Interface) equipment as part of Periodic Safety Review(PSR) of Yonggwang Unit no. 1, 2. As MMI is a key factor among human factors to be reviewed in PSR, we reviewed the MMI components of nuclear power plants in aspect of human factors engineering. The availability, suitability, and effectiveness of the MMI devices were chosen to be reviewed. The MMI devices were investigated through the review of design documents related to the MMI, survey of control panels, evaluation of experts, and experimental assessment. Checklists were used to perform this assessment and record the review results. The items mentioned by the expert comments to review in detail in relation with task procedures were tested by experiments with operators' participation. For some questionable issues arisen during this MMI review, operator workload and possibility of errors in operator actions were analysed. The reviewed MMI devices contain MCR(Main Control Room), SPDS(Safety Parameter Display System), RSP(Remote Shutdown Panel), and the selected LCBs(Local Control Boards) importantly related to safety. As results of the assessments, any significant problem challenging the safety was not found on human factors in the MMI devices. However, several small items to be changed and improved in suitability of MMI devices were discovered. An action plan is recommended to accommodate the suggestions and review comments. It will enhance the plant safety on MMI area

  8. Human Factors Evaluation of Man-Machine Interface for Periodic Safety Review of Yonggwang Unit no. 1, 2

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Yong Hee; Lee, Jung Woon; Park, Jae Chang (and others)

    2006-01-15

    This report describes the research results of human factors assessment on the MMI(Man Machine Interface) equipment as part of Periodic Safety Review(PSR) of Yonggwang Unit no. 1, 2. As MMI is a key factor among human factors to be reviewed in PSR, we reviewed the MMI components of nuclear power plants in aspect of human factors engineering. The availability, suitability, and effectiveness of the MMI devices were chosen to be reviewed. The MMI devices were investigated through the review of design documents related to the MMI, survey of control panels, evaluation of experts, and experimental assessment. Checklists were used to perform this assessment and record the review results. The items mentioned by the expert comments to review in detail in relation with task procedures were tested by experiments with operators' participation. For some questionable issues arisen during this MMI review, operator workload and possibility of errors in operator actions were analysed. The reviewed MMI devices contain MCR(Main Control Room), SPDS(Safety Parameter Display System), RSP(Remote Shutdown Panel), and the selected LCBs(Local Control Boards) importantly related to safety. As results of the assessments, any significant problem challenging the safety was not found on human factors in the MMI devices. However, several small items to be changed and improved in suitability of MMI devices were discovered. An action plan is recommended to accommodate the suggestions and review comments. It will enhance the plant safety on MMI area.

  9. Simulation-based man-machine interface evaluation for plant maintenance facilities

    International Nuclear Information System (INIS)

    Nakagawa, Takashi; Kitamura, Masashi; Nakatani, Yoshio; Umeda, Yoshikazu

    1998-01-01

    Although a lot of human errors has occurred in the maintenance of power plants, systematic approaches to the reduction of human error are not sufficient compared with those applied to operations. The authors propose a new method of evaluating and analyzing human interface design from the viewpoint of human error reduction, and have implemented the method on the DIAS system. This system consists of maintenance personnel and equipment interface simulators, and it generates a dynamic interaction between humans and the working environment. The DIAS system can calculate the human error rate and their weighted calculations according to dangerousness, the influence on the equipment, and the influence on the plant of each task by THERP, and can carry out the dynamic analysis of the panel selection error index if there is similar equipment in the neighborhood, the working time, the distance of movement, and the distance of eye movement. The authors applied this system to evaluate the interface design of actual Transformer Protection Relay Panels and their layout in a room in a nuclear power plant. Our customer accepted our evaluation and proposals modifying the panel design. (author)

  10. Man-Machine Interface System for Neuromuscular Training and Evaluation Based on EMG and MMG Signals

    Directory of Open Access Journals (Sweden)

    Patricia Fernández

    2010-12-01

    Full Text Available This paper presents the UVa-NTS (University of Valladolid Neuromuscular Training System, a multifunction and portable Neuromuscular Training System. The UVa-NTS is designed to analyze the voluntary control of severe neuromotor handicapped patients, their interactive response, and their adaptation to neuromuscular interface systems, such as neural prostheses or domotic applications. Thus, it is an excellent tool to evaluate the residual muscle capabilities in the handicapped. The UVa-NTS is composed of a custom signal conditioning front-end and a computer. The front-end electronics is described thoroughly as well as the overall features of the custom software implementation. The software system is composed of a set of graphical training tools and a processing core. The UVa-NTS works with two classes of neuromuscular signals: the classic myoelectric signals (MES and, as a novelty, the myomechanic signals (MMS. In order to evaluate the performance of the processing core, a complete analysis has been done to classify its efficiency and to check that it fulfils with the real-time constraints. Tests were performed both with healthy and selected impaired subjects. The adaptation was achieved rapidly, applying a predefined protocol for the UVa-NTS set of training tools. Fine voluntary control was demonstrated to be reached with the myoelectric signals. And the UVa-NTS demonstrated to provide a satisfactory voluntary control when applying the myomechanic signals.

  11. Man-machine interface system for neuromuscular training and evaluation based on EMG and MMG signals.

    Science.gov (United States)

    de la Rosa, Ramon; Alonso, Alonso; Carrera, Albano; Durán, Ramon; Fernández, Patricia

    2010-01-01

    This paper presents the UVa-NTS (University of Valladolid Neuromuscular Training System), a multifunction and portable Neuromuscular Training System. The UVa-NTS is designed to analyze the voluntary control of severe neuromotor handicapped patients, their interactive response, and their adaptation to neuromuscular interface systems, such as neural prostheses or domotic applications. Thus, it is an excellent tool to evaluate the residual muscle capabilities in the handicapped. The UVa-NTS is composed of a custom signal conditioning front-end and a computer. The front-end electronics is described thoroughly as well as the overall features of the custom software implementation. The software system is composed of a set of graphical training tools and a processing core. The UVa-NTS works with two classes of neuromuscular signals: the classic myoelectric signals (MES) and, as a novelty, the myomechanic signals (MMS). In order to evaluate the performance of the processing core, a complete analysis has been done to classify its efficiency and to check that it fulfils with the real-time constraints. Tests were performed both with healthy and selected impaired subjects. The adaptation was achieved rapidly, applying a predefined protocol for the UVa-NTS set of training tools. Fine voluntary control was demonstrated to be reached with the myoelectric signals. And the UVa-NTS demonstrated to provide a satisfactory voluntary control when applying the myomechanic signals.

  12. The Human Factors System of VGB. The body of measures implemented by the NPP operating companies for HF management and optimisation of the man-machine interface

    International Nuclear Information System (INIS)

    Eisgruber, H.; Janssen, G.

    1999-01-01

    One way of ensuring high human reliability is to systematically record, analyse and optimise the identified human factors influencing the safety of operation of NPPs. There are two kinds of human factors to be considered: Those identified and characterised in their actual influence on the man-machine system after an event has happened, and the potential human factors, as far as they are known. The purpose of the paper presented is to: - explain the man-machine interface on the basis of the descriptions delivered by work science, as well as with the information model (acquisition, processing and application of information); - explain their influence on the human performance; - identify the organisational units/competences of a nuclear power plant with respect to responsibility for dealing with those factors influencing human performance; - describe suitable joint action of the responsible organisational units; - present results of HF system reviews and modifications. (orig./CB) [de

  13. Data management and communication networks for Man-Machine Interface System in Korea Advanced Liquid MEtal Reactor : its functionality and design requirements

    International Nuclear Information System (INIS)

    Cha, Kyung Ho; Park, Gun Ok; Suh, Sang Moon; Kim, Jang Yeol; Kwon, Kee Choon

    1998-01-01

    The DAta management and Communication NETworks(DACONET), which it is designed as a subsystem for Man-Machine Interface System of Korea Advanced LIquid MEtal Reactor(KALIMER MMIS) and advanced design concept is approached, is described. The DACONET has its roles of providing the real-time data transmission and communication paths between MMIS systems, providing the quality data for protection, monitoring and control of KALIMER and logging the static and dynamic behavioral data during KALIMER operation. The DACONET is characterized as the distributed real-time system architecture with high performance. Future direction, in which advanced technology is being continually applied to Man-Machine Interface System development and communication networks of KALIMER MMIS

  14. Data management and communication networks for Man-Machine Interface System in Korea Advanced Liquid MEtal Reactor : its functionality and design requirements

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Ho; Park, Gun Ok; Suh, Sang Moon; Kim, Jang Yeol; Kwon, Kee Choon [KAERI, Taejon (Korea, Republic of)

    1998-05-01

    The DAta management and Communication NETworks(DACONET), which it is designed as a subsystem for Man-Machine Interface System of Korea Advanced LIquid MEtal Reactor(KALIMER MMIS) and advanced design concept is approached, is described. The DACONET has its roles of providing the real-time data transmission and communication paths between MMIS systems, providing the quality data for protection, monitoring and control of KALIMER and logging the static and dynamic behavioral data during KALIMER operation. The DACONET is characterized as the distributed real-time system architecture with high performance. Future direction, in which advanced technology is being continually applied to Man-Machine Interface System development and communication networks of KALIMER MMIS.

  15. Data management and communication networks for man-machine interface system in Korea Advanced LIquid MEtal Reactor : Its functionality and design requirements

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Ho; Park, Gun Ok; Suh, Sang Moon; Kim, Jang Yeol; Kwon, Kee Choon [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1999-12-31

    The DAta management and COmmunication NETworks(DACONET), which it is designed as a subsystem for Man-Machine Interface System of Korea Advanced LIquid MEtal Reactor (KALIMER MMIS) and advanced design concept is approached, is described. The DACONET has its roles of providing the real-time data transmission and communication paths between MMIS systems, providing the quality data for protection, monitoring and control of KALIMER and logging the static and dynamic behavioral data during KALIMER operation. The DACONET is characterized as the distributed real-time system architecture with high performance. Future direction, in which advanced technology is being continually applied to Man-Machine Interface System development of Nuclear Power Plants, will be considered for designing data management and communication networks of KALIMER MMIS. 9 refs., 1 fig. (Author)

  16. Data management and communication networks for man-machine interface system in Korea Advanced LIquid MEtal Reactor : Its functionality and design requirements

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Ho; Park, Gun Ok; Suh, Sang Moon; Kim, Jang Yeol; Kwon, Kee Choon [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1998-12-31

    The DAta management and COmmunication NETworks(DACONET), which it is designed as a subsystem for Man-Machine Interface System of Korea Advanced LIquid MEtal Reactor (KALIMER MMIS) and advanced design concept is approached, is described. The DACONET has its roles of providing the real-time data transmission and communication paths between MMIS systems, providing the quality data for protection, monitoring and control of KALIMER and logging the static and dynamic behavioral data during KALIMER operation. The DACONET is characterized as the distributed real-time system architecture with high performance. Future direction, in which advanced technology is being continually applied to Man-Machine Interface System development of Nuclear Power Plants, will be considered for designing data management and communication networks of KALIMER MMIS. 9 refs., 1 fig. (Author)

  17. Man as a safety element in the operation of a nuclear power plant. IAEA international conference on man-machine interface in the nuclear industry - review paper

    International Nuclear Information System (INIS)

    Ikegame, R.

    1988-01-01

    Nuclear power generation has been steadily developing worldwide and is now playing a very important part in total energy supply. In order to further promote nuclear power generation harmoniously, it is essential to secure the trust of the public by keeping safe and stable operation and to make constant efforts not to repeat such severe accidents as TMI and Chernobyl. These two accidents have caused us to recognize the substantial importance of the Man-Machine interface. With this as a background, the International Conference on the Man-Machine Interface in the Nuclear Industry was held in Tokyo for four days from February 15 th 1988, organized by IAEA and in cooperation with OECD/NEA as well as CEC. I would like to review this conference, for which I was the Vice Chairman of the Japanese Organizing Committee, and to explain my opinion about this issue

  18. Molecular Interactions in Binary Mixtures of Benzene with 1-Alkanols(C5,C7,C8) at 35℃:An Ultrasonic Study

    Institute of Scientific and Technical Information of China (English)

    ALI,A.; IBRAHIM.M; 等

    2003-01-01

    Densities and ultrasonic speeds have been measured in binary mixtures of benzene with 1-pentanol,1-heptanol and 1-octanol,and in the pure components,as a function of compostion at 35℃.The isentropic compressibility,intermolecular free length,relative association,acoustic impedance,isothermal compressibility,thermal expansion coefficient,deviations in isentropic compressibility,excess freee length,excess volume,deviations in ultrasonic speed,excess acoustic impedance,apparent molar compressibility,apparent molar volume,partial molar volume of 1-alkanol in benzene have been calculated from the experimental data of densities and ultrasonic speeds.The variation of these parameters with composition indicates weak interaction between the component molecules and this interaction decreases in the order:1-pentanol>1-heptanol>1-octanol.Further,theoretical values of ultrasonic speeds were evaluated using free length theory,collision factor theory,Nomoto's relation and Van Dael-Vangeel ideal mixing relation.The relative merits of these theories and relations were discussed for these systems.

  19. The human error rate assessment and optimizing system HEROS - a new procedure for evaluating and optimizing the man-machine interface in PSA

    International Nuclear Information System (INIS)

    Richei, A.; Hauptmanns, U.; Unger, H.

    2001-01-01

    A new procedure allowing the probabilistic evaluation and optimization of the man-machine system is presented. This procedure and the resulting expert system HEROS, which is an acronym for Human Error Rate Assessment and Optimizing System, is based on the fuzzy set theory. Most of the well-known procedures employed for the probabilistic evaluation of human factors involve the use of vague linguistic statements on performance shaping factors to select and to modify basic human error probabilities from the associated databases. This implies a large portion of subjectivity. Vague statements are expressed here in terms of fuzzy numbers or intervals which allow mathematical operations to be performed on them. A model of the man-machine system is the basis of the procedure. A fuzzy rule-based expert system was derived from ergonomic and psychological studies. Hence, it does not rely on a database, whose transferability to situations different from its origin is questionable. In this way, subjective elements are eliminated to a large extent. HEROS facilitates the importance analysis for the evaluation of human factors, which is necessary for optimizing the man-machine system. HEROS is applied to the analysis of a simple diagnosis of task of the operating personnel in a nuclear power plant

  20. Workshop on Interactive Man-Machine Discourse Held at the University of Pennsylvania, 17-20 June 1980,

    Science.gov (United States)

    1980-01-01

    absence of such features, particularly if they are clustered together in a single message, can convey a /AA relaxed tone of familiarity with the receiver... ANALISIS Starr Roxanne Biltz, Kenneth Johnson, and Ann Marie Rabke Upsala College INTRODUCTION A variable of secondary interest La problem type. Much

  1. Task 3: new man-machine interfaces in nuclear power plants. Part 1 executive summary and summary of reports

    International Nuclear Information System (INIS)

    1993-11-01

    The present document summarises the reports provided by the member countries reflecting the actual status of consideration of Human Factors principles for the nuclear power plants. The adoption of Human Factor principles, in conjunction with the availability of advanced digital technology, has already led to the addition of computerised operator aids to almost all the operating plants in the member countries and to the study and the design of fully digitized control rooms for the future plants in some of them. As regards the licensing issue, many uncertainties still exist on the methodology and tools to be used and no internationally accepted standards have been established by now, especially for the software to be used in safety systems of nuclear power plants. The licensing of Man-Machine Interfaces (MMIs) is, in conclusion, at the very beginning of the process as in the past not enough attention has been given to human factor and software licensing aspects. In some countries the preparation of new ad hoc guidelines, or the updating of the existing one for digital equipment, are under way. Several examples of licensing of MMIs in countries where control room systems are safety classified, are described in the report highlighting the different approaches adopted. Important aspects of the design and implementation process of MMIs are the evaluation / validation phase, the provision of advanced alarm system supporting the operator in his supervisory tasks (and avoiding at the same time the problems related to information overload); the use of Artificial Intelligence under the form of Expert Systems is another opportunity that can be applied to several areas of plant supervision and control such as real time diagnostics, decision support, emergency response in order to enhance operator understanding of the plant status in whatever situation and therefore to help him in the operational decision-making process. The proper balance between automation and human actions is

  2. Prospects of a mathematical theory of human behavior in complex man-machine systems tasks. [time sharing computer analogy of automobile driving

    Science.gov (United States)

    Johannsen, G.; Rouse, W. B.

    1978-01-01

    A hierarchy of human activities is derived by analyzing automobile driving in general terms. A structural description leads to a block diagram and a time-sharing computer analogy. The range of applicability of existing mathematical models is considered with respect to the hierarchy of human activities in actual complex tasks. Other mathematical tools so far not often applied to man machine systems are also discussed. The mathematical descriptions at least briefly considered here include utility, estimation, control, queueing, and fuzzy set theory as well as artificial intelligence techniques. Some thoughts are given as to how these methods might be integrated and how further work might be pursued.

  3. Role of C5 Activation Products in Sepsis

    Directory of Open Access Journals (Sweden)

    Peter A. Ward

    2010-01-01

    Full Text Available Complement activation products are known to be generated in the setting of both experimental and human sepsis. C5 activation products (C5a anaphylatoxin and the membrane attack complex [MAC] C5b-9 are generated during sepsis following infusion of endotoxin, or after cecal ligation and puncture (CLP, which produces polymicrobial sepsis. C5a reacts with its receptors C5aR and C5L2 in a manner that creates the “cytokine storm”, and is associated with development of multiorgan failure (MOF. A number of other complications arising from the interaction of C5a with its receptors include apoptosis of lymphoid cells, loss of innate immune functions of neutrophils (PMNs, polymorphonuclear leukocytes, cardiomyopathy, disseminated intravascular coagulation, and complications associated with MOF. Neutralization of C5a in vivo or absence/blockade of C5a receptors greatly reduces the adverse events in the setting of sepsis, markedly attenuates MOF, and greatly improves survival. Regarding the possible role of C5b-9 in sepsis, the literature is conflicting. Some studies suggest that C5b-9 is protective, while other studies suggest the contrary. Clearly, in human sepsis, C5a and its receptors may be logical targets for interception.

  4. The reported incidence of man-machine interface issues in Army aviators using the Aviator's Night Vision System (ANVIS) in a combat theatre

    Science.gov (United States)

    Hiatt, Keith L.; Rash, Clarence E.

    2011-06-01

    Background: Army Aviators rely on the ANVIS for night operations. Human factors literature notes that the ANVIS man-machine interface results in reports of visual and spinal complaints. This is the first study that has looked at these issues in the much harsher combat environment. Last year, the authors reported on the statistically significant (pEnduring Freedom (OEF). Results: 82 Aircrew (representing an aggregate of >89,000 flight hours of which >22,000 were with ANVIS) participated. Analysis demonstrated high complaints of almost all levels of back and neck pain. Additionally, the use of body armor and other Aviation Life Support Equipment (ALSE) caused significant ergonomic complaints when used with ANVIS. Conclusions: ANVIS use in a combat environment resulted in higher and different types of reports of spinal symptoms and other man-machine interface issues over what was previously reported. Data from this study may be more operationally relevant than that of the peacetime literature as it is derived from actual combat and not from training flights, and it may have important implications about making combat predictions based on performance in training scenarios. Notably, Aircrew remarked that they could not execute the mission without ANVIS and ALSE and accepted the degraded ergonomic environment.

  5. Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women

    Science.gov (United States)

    Rezvani, Reza; Gupta, Abhishek; Marceau, Picard; Tchernof, André

    2014-01-01

    Adipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a receptors, classically required for host defense, are also associated with adiposity. Anthropometry and fasting blood parameters were measured in 136 women divided by body mass index (BMI): normal/overweight (≤30 kg/m2; n = 34), obese I (≤45 kg/m2; n = 33), obese II (≤51 kg/m2; n = 33), and obese III (≤80 kg/m2; n = 36). Subcutaneous and omental adipose tissue C5aR and C5L2 expression were analysed. C5L2 expression was comparable between subcutaneous and omental across all BMI groups. Plasma ASP and ASP/omental C5L2 expression increased with BMI (P correlations between C5L2/C5aR and waist circumference, HDL-C, and adiponectin. Tissue and BMI differences in receptors and ligands, particularly in omental, suggest relationship to metabolic disturbances and highlight adipose-immune interactions. PMID:24523571

  6. Man-machine interface design of real-time hardware-in-loop simulation system for power regulation of nuclear heating reactor

    International Nuclear Information System (INIS)

    Ni Xiaoli; Huang Xiaojin; Dong Zhe

    2009-01-01

    It is necessary to set up real-time hardware-in-loop simulation system for power regulation of nuclear heating reactor (NHR) because it is used in the load following instance such as seawater desalination and energy source. As the experiment data are so large that it is hard to be real-time all in one computer and to save and show the data.With the distributed configuration, the system was set up having a legible and intuitionist man-machine interface, speeding the model calculation computer and meeting the requirements of power regulation of NHR. Screen clear and concise, easy command input and results output make the system easier to verify. (authors)

  7. System and Software Design for the Man Machine Interface System for Shin-Hanul Nuclear Power Plant Units 1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Woong Seock; Kim, Chang Ho; Lee, Yoon Hee; Sohn, Se Do; Baek, Seung Min [KEPCO E and C, Daejeon (Korea, Republic of)

    2015-10-15

    The design of the safety MMIS(Man Machine Interface System) system has been performed using POSAFE-Q Programmable Logic Controller (PLC). The design of the non-safety MMIS has been performed using OPERASYSTEM Distributed Control System (DCS). This paper describes the design experiences from the design work of the MMIS using these new platforms. The SHN 1 and 2 MMIS has been developed using POSAFE-Q platform for safety and OPERASYSTEM for non-safety system. Through the utilization of the standardized platform, the safety system was developed using the above hardware and software blocks resulting in efficient safety system development. An integrated CASE tool has been setup for reliable software development. The integrated development environment has been setup formally resulting in consistent work. Even we have setup integrated development environment, the independent verification and validation including testing environment needs to be setup for more advanced environment which will be used for future plant.

  8. Development of AI (Artificial Intelligence)-based simulation system for man-machine system behavior in accidental situations of nuclear power plant

    International Nuclear Information System (INIS)

    Yoshida, Kazuo; Yokobayashi, Masao; Tanabe, Fumiya; Kawase, Katumi.

    1996-01-01

    A prototype version of a computer simulation system named JACOS (JAeri COgnitive Simulation system) has been developed at JAERI (Japan Atomic Energy Research Institute) to simulate the man-machine system behavior in which both the cognitive behavior of a human operator and the plant behavior affect each other. The objectives of this system development is to provide man-machine system analysts with detailed information on the cognitive process of an operator and the plant behavior affected by operator's actions in accidental situations of an NPP (nuclear power plant). The simulation system consists of an operator model and a plant model which are coupled dynamically. The operator model simulates an operator's cognitive behavior in accidental situations based on the decision ladder model of Rasmussen, and is implemented using the AI-techniques of the distributed cooperative inference method with the so-called blackboard architecture. Rule-based behavior is simulated using knowledge representation with If-Then type of rules. Knowledge-based behavior is simulated using knowledge representation with MFM (Multilevel Flow Modeling) and qualitative reasoning method. Cognitive characteristics of attentional narrowing, limitation of short-term memory, and knowledge recalling from long-term memory are also modeled. The plant model of a 3-loop PWR is also developed using a best estimate thermal-hydraulic analysis code RELAP5/MOD2. Some simulations of incidents were performed to verify the human model. It was found that AI-techniques used in the human model are suitable to simulate the operator's cognitive behavior in an NPP accident. The models of cognitive characteristics were investigated in the effects on simulated results of cognitive behaviors. (author)

  9. C5 capsule operation modes analysis

    International Nuclear Information System (INIS)

    Negut, Gh.; Ancuta, Mirela; Stefan, Violeta

    2008-01-01

    This paper is part of the Nuclear Research Institute Program 13 dedicated to 'TRIGA Research Reactor performance enhancing' and its objective is improving the engineering of the structural materials irradiation. The paper raises the knowledge level on C5 capsule irradiation modes and utilizes previous results in order to increase C5 performances. In the paper the irradiation modes to test zirconium yttrium sample are assessed. These tests are proposed by AECL. There are presented the C5 initial conditions and models. Also. there are presented the thermal hydraulic conditions during normal and accidental operation. The results will be used in the C5 safety report. (authors)

  10. On action- and affectpsychology of human reliability. An access by training simulators for complex man-machine systems

    International Nuclear Information System (INIS)

    Schuette, M.

    2002-02-01

    Theoretical part and its topics: errors at the interface between man and machine; reliability analysis for man; the psychological explanation of action reliability of man (intention and control); a paradigma for human reliability (frustration and regression). Empirical part: Control room in a nuclear power plant: Influences on repeated blockages on component care in case of start-up operation; ship bridge: Frustration and regression while steering in a bight. Appendix: analysis of a social interaction.(GL)

  11. The proposed human factors engineering program plan for man-machine interface system design of the next generation NPP in Korea

    International Nuclear Information System (INIS)

    Oh, I.S.; Lee, H.C.; Seo, S.M.; Cheon, S.W.; Park, K.O.; Lee, J.W.; Sim, B.S.

    1994-01-01

    Human factors application to nuclear power plant (NPP) design, especially, to man-machine interface system (MMIS) design becomes an important issue among the licensing requirements. Recently, the nuclear regulatory bodies require the evidence of systematic human factors application to the MMIS design. Human Factors Engineering Program Plan (HFEPP), as a basis and central one among the human factors application by the MMIS designers. This paper describes the framework of HFEPP for the MMIS design of next generation NPP (NG-NPP) in Korea. This framework provides an integral plan and some bases of the systematic application of human factors to the MMIS design, and consists of purpose and scope, codes and standards, human factors organization, human factors tasks, engineering control methodology, human factors documentations, and milestones. The proposed HFEPP is a top level document to define and describe human factors tasks, based on each step of MMIS design process, in view point of how, what, when and by whom to be performed. (author). 11 refs, 1 fig

  12. SL(C) 5 migration at CERN

    International Nuclear Information System (INIS)

    Schwickerath, Ulrich; Silva, Ricardo

    2010-01-01

    Most LCG sites are currently running on SL(C)4. However, this operating system is already rather old, and it is becoming difficult to get the required hardware drivers, to get the best out of recent hardware. A possible way out is the migration to SL(C)5 based systems where possible, in combination with virtualization methods. The former is typically possible for nodes where the software to run the services is available and tested, while the latter offers a possibility to make use of the new hardware platforms whilst maintaining operating system compatibility. Since autumn 2008, CERN has offered public interactive and batch worker nodes for evaluation to the experiments. For the Grid environment, access is granted by a dedicated CEs. The status of the evaluation, feedback received from the experiments and the status of the migration will be reviewed, and the status of virtualization of services at CERN will be reported. Beyond this, the migration to a new operating system also offers an excellent opportunity to upgrade the fabric infrastructure used to manage the servers.

  13. Multithreading in C# 5.0 cookbook

    CERN Document Server

    Agafonov, Eugene

    2013-01-01

    Cookbook.Multithreading in C# 5.0 Cookbook is written for existing C# developers who want a complete, professional, and authoritative guide to multithreading. You don't need any experience with multithreaded programming to use this book.

  14. 76 FR 69636 - Amides, C5

    Science.gov (United States)

    2011-11-09

    ... in guinea pigs showed that amides, C 5 - C 9 , N-[3-(dimethylamino) propyl] was not a skin sensitizer.... Potentially affected entities may include, but are not limited to: Crop production (NAICS code 111). Animal production (NAICS code 112). Food manufacturing (NAICS code 311). Pesticide manufacturing (NAICS code 32532...

  15. 4th International Conference on Man–Machine Interactions

    CERN Document Server

    Brachman, Agnieszka; Kozielski, Stanisław; Czachórski, Tadeusz

    2016-01-01

    This book provides an overview of the current state of research on development and application of methods, algorithms, tools and systems associated with the studies on man-machine interaction. Modern machines and computer systems are designed not only to process information, but also to work in dynamic environment, supporting or even replacing human activities in areas such as business, industry, medicine or military. The interdisciplinary field of research on man-machine interactions focuses on broad range of aspects related to the ways in which human make or use computational artifacts, systems and infrastructure.   This monograph is the fourth edition in the series and presents new concepts concerning analysis, design and evaluation of man-machine systems. The selection of high-quality, original papers covers a wide scope of research topics focused on the main problems and challenges encountered within rapidly evolving new forms of human-machine relationships. The presented material is structured into fol...

  16. 29 CFR 2560.502c-5 - Civil penalties under section 502(c)(5).

    Science.gov (United States)

    2010-07-01

    ... Act) that is not a group health plan, and that provides benefits consisting of medical care (within... section 502(c)(5) of the Act for each failure or refusal to file a completed report required to be filed...). (2) For purposes of this section, a failure or refusal to file the report required to be filed under...

  17. Theoretical study of the decomposition pathways and products of C5- perfluorinated ketone (C5 PFK)

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Yuwei; Wang, Xiaohua, E-mail: xhw@mail.xjtu.edu.cn, E-mail: mzrong@mail.xjtu.edu.cn; Li, Xi; Yang, Aijun; Wu, Yi; Rong, Mingzhe, E-mail: xhw@mail.xjtu.edu.cn, E-mail: mzrong@mail.xjtu.edu.cn [State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, No. 28 XianNing West Road, Xi’an, Shaanxi Province 710049 (China); Han, Guohui; Lu, Yanhui [Pinggao Group Co. Ltd., Pingdingshan, Henan Province 467001 (China)

    2016-08-15

    Due to the high global warming potential (GWP) and increasing environmental concerns, efforts on searching the alternative gases to SF{sub 6}, which is predominantly used as insulating and interrupting medium in high-voltage equipment, have become a hot topic in recent decades. Overcoming the drawbacks of the existing candidate gases, C5- perfluorinated ketone (C5 PFK) was reported as a promising gas with remarkable insulation capacity and the low GWP of approximately 1. Experimental measurements of the dielectric strength of this novel gas and its mixtures have been carried out, but the chemical decomposition pathways and products of C5 PFK during breakdown are still unknown, which are the essential factors in evaluating the electric strength of this gas in high-voltage equipment. Therefore, this paper is devoted to exploring all the possible decomposition pathways and species of C5 PFK by density functional theory (DFT). The structural optimizations, vibrational frequency calculations and energy calculations of the species involved in a considered pathway were carried out with DFT-(U)B3LYP/6-311G(d,p) method. Detailed potential energy surface was then investigated thoroughly by the same method. Lastly, six decomposition pathways of C5 PFK decomposition involving fission reactions and the reactions with a transition states were obtained. Important intermediate products were also determined. Among all the pathways studied, the favorable decomposition reactions of C5 PFK were found, involving C-C bond ruptures producing Ia and Ib in pathway I, followed by subsequent C-C bond ruptures and internal F atom transfers in the decomposition of Ia and Ib presented in pathways II + III and IV + V, respectively. Possible routes were pointed out in pathway III and lead to the decomposition of IIa, which is the main intermediate product found in pathway II of Ia decomposition. We also investigated the decomposition of Ib, which can undergo unimolecular reactions to give the

  18. 26 CFR 1.381(c)(5)-1 - Inventories.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Inventories. 1.381(c)(5)-1 Section 1.381(c)(5)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Insolvency Reorganizations § 1.381(c)(5)-1 Inventories. (a) Carryover requirement—(1...

  19. Nano-jewellery: C5Au12--a gold-plated diamond at molecular level.

    Science.gov (United States)

    Naumkin, F

    2006-06-07

    A mixed carbon-metal cluster is designed by combining the tetrahedral C(5) radical (with a central atom-the skeleton of the C(5)H(12) molecule) and the spherical Au(12) layer (the external atomic shell of the Au(13) cluster). The C(5)Au(12) cluster and its negative and positive ionic derivatives, C(5)Au(12)(+/-), are investigated ab initio (DFT) in terms of optimized structures and relative energies of a few spin-states, for the icosahedral-like and octahedral-like isomers. The cluster is predicted to be generally more stable in its octahedral shape (similar to C(5)H(12)) which prevails for the negative ion and may compete with the icosahedral shape for the neutral system and positive ion. Adiabatic ionization energies (AIE) and electron affinities (AEA) of C(5)Au(12), vertical electron-detachment (VDE) energies of C(5)Au(12)(-), and vertical ionization and electron-attachment energies (VIE, VEA) of C(5)Au(12) are calculated as well, and compared with those for the corresponding isomers of the Au(13) cluster. The AIE and VIE values are found to be close for the two systems, while the AEA and VDE values are significantly reduced for the radical-based species. A simple fragment-based model is proposed for the decomposition of the total interaction into carbon-gold and gold-gold components.

  20. Speech Analysis and Synthesis and Man-Machine Speech Communications for Air Operations. (Synthese et Analyse de la Parole et Liaisons Vocales Homme- Machine dans les Operations Aeriennes)

    Science.gov (United States)

    1990-05-01

    speech processing area are faced . He presents speech communication as an interactive process, in which the listener actively reconstructs the message...speech produced by these systems. Finally, perhaps the greatest recent impetus in advancing digital Finally, in the area of speech and speaker recognitio

  1. A method for classification of network traffic based on C5.0 Machine Learning Algorithm

    DEFF Research Database (Denmark)

    Bujlow, Tomasz; Riaz, M. Tahir; Pedersen, Jens Myrup

    2012-01-01

    current network traffic. To overcome the drawbacks of existing methods for traffic classification, usage of C5.0 Machine Learning Algorithm (MLA) was proposed. On the basis of statistical traffic information received from volunteers and C5.0 algorithm we constructed a boosted classifier, which was shown...... and classification, an algorithm for recognizing flow direction and the C5.0 itself. Classified applications include Skype, FTP, torrent, web browser traffic, web radio, interactive gaming and SSH. We performed subsequent tries using different sets of parameters and both training and classification options...

  2. IDES: Interactive Data Entry System: a generalized data acquisition software package

    International Nuclear Information System (INIS)

    Gasser, S.B.

    1980-04-01

    The Interactive Data Entry System (IDES) is a software package which greatly assists in designing and storing forms to be used for the directed acquisition of data. Objective of this package is to provide a viable man/machine interface to any comprehensive data base. This report provides a technical description of the software and can be used as a user's manual

  3. A bonding study of c-C5H8 adsorption on Pt(111)

    International Nuclear Information System (INIS)

    Simonetti, S.; Jasen, P.; Gonzalez, E.; Juan, A.; Brizuela, G.

    2006-01-01

    The chemisorption of cyclopentane (c-C 5 H 8 ) on Pt(111) has been studied using a qualitative band-structure calculations in the framework of tight-binding implementation with the YAeHMOP package. We modeled the metal surface by a two-dimensional slab of finite thickness with an overlayer of c-C 5 H 8 , in a (3x3) di-σ geometry. The c-C 5 H 8 molecule is attached to the surface with its C?C atoms bonded mainly with two Pt atoms while the opposite CH 2 bends towards the surface. The Pt?Pt bonds in the underlying surface and the C?C bonds of c-C 5 H 8 are weakened upon the chemisorption. A noticeable Pt-H and Pt-C interactions has been observed. We found that of Pt 5d z 2 band plays an important role in the bonding between c-C 5 H 8 and the surface, as do the Pt 6s and 6p z bands. The HOMO-LUMO bands of c-C 5 H 8 are very dispersed, indicative of a strong interaction with the metal surface

  4. Enlarged Halden programme group meeting on high burn-up fuel performance, safety and reliability and degradation of in-core materials and water chemistry effects and man-machine systems research. Volume II

    International Nuclear Information System (INIS)

    1999-01-01

    An Enlarged Halden Programme Group Meeting on Fuel Performance and Materials Testing and Man-Machine Research was held at Loen, Norway 24 to 29 May, 1999. The papers presented covered work performed at the OECD Halden Reactor Project during the years up to April 1999 as well as studies conducted within organisations participating in the Halden Project. The Halden Boiling Water Reactor, HBWR, was built in the years 1955 - 1958 by the Norwegian Institutt for Atomenergi, and has since 1958 been operated internationally as one of the joint undertakings of the OECD Nuclear Energy Agency. The experimental operation of the Halden Boiling Water Reactor and associated programmes are sponsored through an international agreement by Institute for energy technology, Norway, the Belgian Nuclear Research Centre SCK/CEN, acting also on behalf of other public or private organisations in Belgium, the Risoe National Laboratory in Denmark, the Finnish Ministry of Trade and Industry, the Electricite de France, the Gesellschaft fur Anlagen- und Reaktorsicherheit (GRS) mbH, representing a German group of companies working in agreement with the German Federal Ministry for Education, Science, Research and Technology, the Italian Ente per le Nouve Tecnologie, l'Energia e l'Ambiente, the Japan Atomic Energy Research Institute, the Korean Atomic Energy Research Institute, the Spanish Centre de Investigaciones Energeticas, Medioambientales y Tecnologias, representing a group of national and industry organisations in Spain, the Swedish Nuclear Power Inspectorate, the Swiss Federal Nuclear Safety Inspectorate, representing also the Swiss nuclear utilities and the Paul Scherrer Institute, the Nuclear Electric pic, representing a group of nuclear research and industry organisations in the United Kingdom, and the United States Nuclear Regulatory Commission, and as associated parties: the Brazilian Comissao Nacional de Energia Nuclear (CNEN), the Czech Nuclear Research Institute, the Hungarian

  5. The driver in the 21st century. Demands, applications, aspects of man-machine systems. Lectures; Der Fahrer im 21. Jahrhundert. Anforderungen, Anwendungen, Aspekte fuer Mensch-Maschine-Systeme. Plenarvortraege

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    A high grade of mobility is the basis of our economic development. The innovations in the automotive- and deliver industry lead to different fulfillments of customer expectations on vehicle operation. Also in the railway sector increasing requirements on the operators involve a change of view within design of man-machine interfaces. The transmission of information and transfer of reactions require advanced and balanced concepts for operating and monitoring of modern automobiles. The integral connection of competence in technology, design and ergonomics, sense- and traffic psychology as well as legal problems lead to new challenges and possibilities. (GL) [German] Der Fahrer im 21. Jahrhundert - Anforderungen, Anwendungen, Aspekte fuer Mensch-Maschine-Systeme - unter diesem Motto wird die interessante Fachtagung ''Der Fahrer im 21. Jahrhundert'' fortgefuehrt. Ein hohes Mass an Mobilitaet ist Grundlage unserer wirtschaftlichen Entwicklung. Die Innovationen in der Automobil- und Zuliefererindustrie fuehren zu einer differenzierten Erfuellung der Kundenerwartungen an die Bedienung der Fahrzeuge. Auch im Bereich Bahn bedingen die steigenden Anforderungen an die Fahrzeugfuehrer ein Umdenken bei der Gestaltung der Mensch-Maschine-Schnittstelle. Die Vermittlung von Informationen und die Umsetzung adaequater Reaktionen erfordern neue und ausgewogene Konzepte fuer das Bedienen und Beobachten moderner Fahrzeuge. Die integrale Verbindung von Kompetenz in Technologie, Design und Ergonomie, Wahrnehmungs- und Verkehrspsychologie sowie rechtlichen Fragestellungen fuehren hier zu neuen Herausforderungen und Moeglichkeiten. Die Tagung moechte wieder visionaere Loesungsansaetze aufzeigen und auch das Machbare diskutieren. Nutzen Sie die Gelegenheit, mit namhaften Referenten aus Industrie und Wissenschaft, Fach- und Fuehrungskraeften zu sprechen und auf dieser Veranstaltung neue Bedien- und Informationskonzepte, Teilloesungen fuer die unterschiedlichen Fahraufgaben

  6. Modernization of the subsystem man-machine interface for operation of the combined cycle central of Gomez Palacio; Modernizacion del subsistema de interfaz hombre-maquina para operacion de la central de ciclo combinado de Gomez Palacio

    Energy Technology Data Exchange (ETDEWEB)

    Rivas S, Alfonso; Uribe B, Carlos E; Alvarez C, Victor [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2002-07-01

    In order to take care of the modernization of the Combined Cycle Central of Gomez Palacio (CCCGP) of the Comision Federal de Electricidad (CFE), the Instituto de Investigaciones Electricas (IIE), through the Management of Supervision of Processes (GSP) developed the subsystem of Interface Man-Machine (IMM), to replace the workstations that are deteriorated and obsolete by personal computers, maintaining the same functions of the IMM of the present system, including the management and processing functions that at the present time another system makes. Also, to replace the equipment of the concentrators of communications by a PC with greater capacity, than allows improving in general the flexibility and robustness of the system. Among the main functions integrated in the IMM subsystem there are: the communications master with the controllers (equipment SAC), redundancy of servers, database, historical registry storage, alarm management, tabulations, tendency graphs in real time and historical, process diagrams and Control Panels. [Spanish] Con el objeto de atender las necesidades de modernizacion de la Central de Ciclo Combinado Gomez Palacio (CCCGP) de la Comision Federal de Electricidad (CFE), el Instituto de Investigaciones Electricas (IIE), a traves de la Gerencia de Supervision de Procesos (GSP) desarrollo el subsistema de Interfaz Hmbre-Maquina (IHM), para reemplazar las estaciones de trabajo que se encuentran deterioradas y obsoletas por computadoras personales, manteniendo las mismas funciones de la IHM del sistema actual, incluyendo las funciones de administracion y procesamiento que en la actualidad realiza otro sistema. Asimismo, para reemplazar los equipos de los concentradores de comunicaciones por PC con mayor capacidad, que permitan mejorar la flexibilidad y robustez del sistema en general. Entre las principales funciones integradas en el subsistema de IHM se encuentran: el Maestro de comunicaciones con los controladores (equipo SAC), redundancia de

  7. Efficiency in man-machine communication

    NARCIS (Netherlands)

    Haakma, R.; Engel, F.L.

    1990-01-01

    Expressed in terms of speed and accuracy, intention transfer in goal-oriented inter-human communication can be very efficient. One of the mechanisms that make for efficient communication is early detection and repair of communication errors. Another important efficiency mechanism prevents repeated

  8. Protective effects of C5a blockade in sepsis

    DEFF Research Database (Denmark)

    Czermak, B J; Sarma, V; Pierson, C L

    1999-01-01

    in vitro had greatly reduced production of H2O2, which is known to be essential for the bactericidal function of neutrophils. In contrast, when companion CLP rats were treated with IgG antibody against C5a, survival rates were significantly improved, levels of bacteremia were considerably reduced...

  9. Selective terminal C–C scission of C5-carbohydrates

    NARCIS (Netherlands)

    Klis, van der F.; Gootjes, L.; Haveren, van J.; Es, van D.S.; Bitter, J.H.

    2015-01-01

    The selective catalytic production of C4-tetritols (erythritol and threitol) from C5-sugars is an attractive route for the conversion of non-digestible sugars to C4-building blocks from agro residues. Here we show that an unprecedented high selectivity of 20–25% C4-tertritols can be achieved under

  10. HSV neutralization by the microbicidal candidate C5A

    NARCIS (Netherlands)

    de Witte, L.; Bobardt, M.D.; Chatterji, U.; van Loenen, F.B.; Verjans, G.M.G.M.; Geijtenbeek, T.B.H.; Gallay, P.A.

    2011-01-01

    Genital herpes is a major risk factor in acquiring human immunodeficiency virus type-1 (HIV-1) infection and is caused by both Herpes Simplex virus type 1 (HSV-1) and HSV-2. The amphipathic peptide C5A, derived from the non-structural hepatitis C virus (HCV) protein 5A, was shown to prevent HIV-1

  11. HSV neutralization by the microbicidal candidate C5A

    NARCIS (Netherlands)

    L. de Witte (Lot); M.D. Bobardt (Michael); U. Chatterji (Udayan); F.B. van Loenen (Freek); G.M.G.M. Verjans (George); T.B.H. Geijtenbeek (Teunis); P.A. Gallay (Philippe)

    2011-01-01

    textabstractGenital herpes is a major risk factor in acquiring human immunodeficiency virus type-1 (HIV-1) infection and is caused by both Herpes Simplex virus type 1 (HSV-1) and HSV-2. The amphipathic peptide C5A, derived from the non-structural hepatitis C virus (HCV) protein 5A, was shown to

  12. Synthetic of Zr2Al3C5 material

    International Nuclear Information System (INIS)

    Leela-Adisorn, U.; Yamaguchi, A.

    2005-01-01

    Synthesis method of Zr 2 Al 3 C 5 via solid state reaction between Al, ZrC and carbon powder was studied. Al-ZrC-C compact with equivalent mol ratio of Zr 2 Al 3 C 5 was heated up to 1600 C in Ar atmosphere for 1 h and 4 h but ZrC phase still existed as major phase with very small amount of Zr 2 Al 3 C 5 . Because ZrC started to oxidize at low temperature under very low oxygen partial pressure, the same mol ratio of Al-ZrC-C compact was heated at 1600 C in vacuum for 1 h as parallel test. After firing in vacuum, some carbon still exist with small amount of AlZrC 2 occurred with Zr 2 Al 3 C 5 as a main phase, but no ZrC was found. Different result from firing in Ar atmosphere and in vacuum had been discussed here. It was believed that very small amount of impurities in Ar had some effect on the formation of Al-Zr-C compound. The effect of very small amount of impurities in Ar was studied by thermal analysis (DTA/TG) and XRD. It was found that very small amount of impurities in Ar has effect on the reaction between Al, ZrC and carbon by diffusion through the surface and form Zr-C-O-N solid solution. This solid solution cannot differentiate from ZrC by XRD. With help of thermal analysis method (DTA/TG), Zr-C-O-N solid solution can be differentiated from ZrC. Therefore, synthesis of Al-Zr-C compound should be done in vacuum. Zr 2 Al 3 C 5 can be prepared from mixture of Al-ZrC-C with excess amount of Al at 1600 C for 1 h. (orig.)

  13. C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.

    Directory of Open Access Journals (Sweden)

    Andrea Conroy

    Full Text Available Placental malaria (PM is a leading cause of maternal and infant mortality. Although the accumulation of parasitized erythrocytes (PEs and monocytes within the placenta is thought to contribute to the pathophysiology of PM, the molecular mechanisms underlying PM remain unclear. Based on the hypothesis that excessive complement activation may contribute to PM, in particular generation of the potent inflammatory peptide C5a, we investigated the role of C5a in the pathogenesis of PM in vitro and in vivo.Using primary human monocytes, the interaction between C5a and malaria in vitro was assessed. CSA- and CD36-binding PEs induced activation of C5 in the presence of human serum. Plasmodium falciparum GPI (pfGPI enhanced C5a receptor expression (CD88 on monocytes, and the co-incubation of monocytes with C5a and pfGPI resulted in the synergistic induction of cytokines (IL-6, TNF, IL-1beta, and IL-10, chemokines (IL-8, MCP-1, MIP1alpha, MIP1beta and the anti-angiogenic factor sFlt-1 in a time and dose-dependent manner. This dysregulated response was abrogated by C5a receptor blockade. To assess the potential role of C5a in PM, C5a plasma levels were measured in malaria-exposed primigravid women in western Kenya. Compared to pregnant women without malaria, C5a levels were significantly elevated in women with PM.These results suggest that C5a may contribute to the pathogenesis of PM by inducing dysregulated inflammatory and angiogenic responses that impair placental function.

  14. Typing and grading of cytological category C5 breast lesions

    International Nuclear Information System (INIS)

    Zafar, N.; Jamal, S.; Mamoon, N.; Luqman, M.; Anwar, M.

    2005-01-01

    Objective: To determine the tumour type, cytological grade and nuclear grade on fine-needle aspiration smears in cytological category C5 breast lesions and compare them with histopathological findings. Subjects and Methods: Out of all patients referred to AFIP, Rawalpindi for fine-needle aspiration of breast masses, those adjudged C5 (malignant) were chosen for this study. History, clinical details and mammographic findings were noted. Aspirated smears were examined and an attempt was made to ascertain tumour type, cytological grade and nuclear grade. On excision of these lesions, the cytological findings were compared with those on paraffin-embedded histological sections. Results: A total of 71 patients were included in this study. Of these, 64 (90.14%) were cytologically diagnosed as ductal carcinoma, 4 (5.63%) lobular carcinoma, 2 (2.82%) mucinous carcinoma and 1 (1.41%) as medullary carcinoma. Seventy (98.60%) tumours were correctly typed on aspiration smears. Sixty-eight (95.77%) cases were cytologically graded with accuracy. Nuclear grading was even better on cytology and, excluding one malignant lymphoma, all 70 (100%) smears were assessed correctly. Conclusion: Overall efficiency of the tumour typing, cytological grading and nuclear grading on aspirated material turned out to be quite accurate. In expert hands, cytological examination can be of great help in pre-operative planning and in cases where tumour morphology in paraffin-embedded material has been distorted by neo-adjuvant therapy prior to excision. (author)

  15. C5a and C5aR are elevated in joints of rheumatoid and psoriatic arthritis patients, and C5aR blockade attenuates leukocyte migration to synovial fluid

    DEFF Research Database (Denmark)

    Hornum, Lars; Hansen, Anker Jon; Tornehave, Ditte

    2017-01-01

    synovial fluid was significantly inhibited by anti-C5aR. The data support that the C5a-C5aR axis may be driving the infiltration of inflammatory cells into the synovial fluid and synovium in both rheumatoid and psoriatic arthritis, and suggest that C5a or C5aR may be a promising treatment target in both...... a Boyden chamber. Appropriate statistical tests were applied for comparisons. C5aR+ cells were detected in most rheumatoid arthritis, in all psoriatic arthritis, but not in non-inflammatory control synovia. C5aR+ cells were primarily neutrophils and macrophages. C5aR+ macrophages were mainly found...

  16. Bis(tetramethylcyclopentadienyl)titanium chemistry. Molecular structures of [(C(5)HMe(4))(mu-eta(1) : eta(5)-C(5)Me(4))Ti](2) and [(C(5)HMe(4))(2)Ti]N-2(2)

    NARCIS (Netherlands)

    deWolf, JM; Blaauw, R; Meetsma, A; Teuben, JH; Gyepes, R; Varga, [No Value; Mach, K; Veldman, N; Spek, AL

    1996-01-01

    Thermolysis of bis(tetramethylcyclopentadienyl)-stabilized titanium(III) compounds (C(5)HMe(4))(2)TiR (R = Me (2), Ph (3)) yields, in marked contrast with the bis(pentamethylcyclopentadienyl) analog, the dimeric product [(C(5)HMe(4))(mu-eta(1):eta(5)-C(5)Me(4))Ti](2) (4), With a bridging metalated

  17. The Hydrogen Bonded Structures of Two 5-Bromobarbituric Acids and Analysis of Unequal C5–X and C5–X′ Bond Lengths (X = X′ = F, Cl, Br or Me in 5,5-Disubstituted Barbituric Acids

    Directory of Open Access Journals (Sweden)

    Thomas Gelbrich

    2016-04-01

    Full Text Available The crystal structure of the methanol hemisolvate of 5,5-dibromobarbituric acid (1MH displays an H-bonded layer structure which is based on N–H∙∙∙O=C, N–H∙∙∙O(MeOH and (MeOHO–H∙∙∙O interactions. The barbiturate molecules form an H-bonded substructure which has the fes topology. 5,5′-Methanediylbis(5-bromobarbituric acid 2, obtained from a solution of 5,5-dibromobarbituric acid in nitromethane, displays a N–H···O=C bonded framework of the sxd type. The conformation of the pyridmidine ring and the lengths of the ring substituent bonds C5–X and C5–X′ in crystal forms of 5,5-dibromobarbituric acid and three closely related analogues (X = X′ = Br, Cl, F, Me have been investigated. In each case, a conformation close to a C5-endo envelope is correlated with a significant lengthening of the axial C5–X′ in comparison to the equatorial C5–X bond. Isolated molecule geometry optimizations at different levels of theory confirm that the C5-endo envelope is the global conformational energy minimum of 5,5-dihalogenbarbituric acids. The relative lengthening of the axial bond is therefore interpreted as an inherent feature of the preferred envelope conformation of the pyrimidine ring, which minimizes repulsive interactions between the axial substituent and pyrimidine ring atoms.

  18. C-5 Propynyl Modifications Enhance the Mechanical Stability of DNA.

    Science.gov (United States)

    Aschenbrenner, Daniela; Baumann, Fabian; Milles, Lukas F; Pippig, Diana A; Gaub, Hermann E

    2015-07-20

    Increased thermal or mechanical stability of DNA duplexes is desired for many applications in nanotechnology or -medicine where DNA is used as a programmable building block. Modifications of pyrimidine bases are known to enhance thermal stability and have the advantage of standard base-pairing and easy integration during chemical DNA synthesis. Through single-molecule force spectroscopy experiments with atomic force microscopy and the molecular force assay we investigated the effect of pyrimidines harboring C-5 propynyl modifications on the mechanical stability of double-stranded DNA. Utilizing these complementary techniques, we show that propynyl bases significantly increase the mechanical stability if the DNA is annealed at high temperature. In contrast, modified DNA complexes formed at room temperature and short incubation times display the same stability as non-modified DNA duplexes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. C5 Extract Induces Apoptosis in B16F10 Murine Melanoma Cells ...

    African Journals Online (AJOL)

    cancer activities of C5 extract (C5E), a new herbal preparation from ..... Figure 1: Composition of C5E was analyzed by GC-MS. Among the volatile compounds in C5E, .... body systems because dysfunction of apoptotic signaling could cause serious ...

  20. The C5a/C5aR1 axis controls the development of experimental allergic asthma independent of LysM-expressing pulmonary immune cells.

    Directory of Open Access Journals (Sweden)

    Anna V Wiese

    Full Text Available C5a regulates the development of maladaptive immune responses in allergic asthma mainly through the activation of C5a receptor 1 (C5aR1. Yet, the cell types and the mechanisms underlying this regulation are ill-defined. Recently, we described increased C5aR1 expression in lung tissue eosinophils but decreased expression in airway and pulmonary macrophages as well as in pulmonary CD11b+ conventional dendritic cells (cDCs and monocyte-derived DCs (moDCs during the allergic effector phase using a floxed green fluorescent protein (GFP-C5aR1 knock-in mouse. Here, we determined the role of C5aR1 signaling in neutrophils, moDCs and macrophages for the pulmonary recruitment of such cells and the importance of C5aR1-mediated activation of LysM-expressing cells for the development of allergic asthma. We used LysM-C5aR1 KO mice with a specific deletion of C5aR1 in LysMCre-expressing cells and confirmed the specific deletion of C5aR1 in neutrophils, macrophages and moDCs in the airways and/or the lung tissue. We found that alveolar macrophage numbers were significantly increased in LysM-C5aR1 KO mice. Induction of ovalbumin (OVA-driven experimental allergic asthma in GFP-C5aR1fl/fl and LysM-C5aR1 KO mice resulted in strong but similar airway resistance, mucus production and Th2/Th17 cytokine production. In contrast, the number of airway but not of pulmonary neutrophils was lower in LysM-C5aR1 KO as compared with GFP-C5aR1fl/fl mice. The recruitment of macrophages, cDCs, moDCs, T cells and type 2 innate lymphoid cells was not altered in LysM-C5aR1 KO mice. Our findings demonstrate that C5aR1 is critical for steady state control of alveolar macrophage numbers and the transition of neutrophils from the lung into the airways in OVA-driven allergic asthma. However, C5aR1 activation of LysM-expressing cells plays a surprisingly minor role in the recruitment and activation of such cells and the development of the allergic phenotype in OVA-driven experimental

  1. Naked (C5Me5)(2)M cations (M = Sc, Ti, and V) and their fluoroarene complexes

    NARCIS (Netherlands)

    Bouwkamp, MW; Budzelaar, PHM; Gercama, J; Morales, ID; de Wolf, J; Meetsma, A; Troyanov, SI; Teuben, JH; Hessen, B; Budzelaar, Peter H.M.; Hierro Morales, Isabel Del; Troyanov, Sergei I.

    2005-01-01

    The ionic metallocene complexes [Cp*M-2][BPh4] (CP* = C5Me5) of the trivalent 3d metals Sc, Ti, and V were synthesized and structurally characterized. For M Sc, the anion interacts weakly with the metal center through one of the phenyl groups, but for M = Ti and V, the cations are naked. They each

  2. Prediction of compatibility of crude oils with condensate (C5+); Previsao de compatibilidade de petroleos e condensado (C5+)

    Energy Technology Data Exchange (ETDEWEB)

    Zilio, Evaldo Lopez; Santos, Maria de Fatima Pereira dos [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil); Ramos, Antonio Carlos da Silva; Rolemberg, Marlus Pinheiro [Universidade Federal do Maranhao (UFMA), Sao Luis, MA (Brazil)

    2008-07-01

    Due to the recent raise of the national natural gas demand and to the need of flowing the condensates (C5+) produced from the NGPP (Natural Gas Processing Plant) by adding them to the streams of the crude oil, there was the need to carry out the compatibility prediction of one condensate with two onshore crude oils from Espirito Santo. The model to predict the compatibility among crude oils and among crude oils and oil products is based on the use of the solubility parameter of the oils. To apply it, the solubility parameter of each crude oil or oil product is measured and the parameter of their blend is calculated. If this value is beneath the asphaltenes flocculation parameter, the blend is incompatible; if it is above, the blend is compatible. In this article, the compatibility predictions were done according to the Solubility Parameter Model to two blends: the condensate C with the crude oil X and with the crude oil Y. The model predictions are that both blends are incompatible at given proportions. To check the predictions, the same two blends were experimentally carried out. It must be emphasized that the compatibility tests were done at atmospheric pressure and at the temperature of 15 deg C. These tests consist in adding the condensate to the crude oil with a titrater and visualizing the asphaltenes precipitation at an optical microscope. The experimental results were equivalent to the values predicted by the model. It is worth mentioning that there were several practical difficulties, as the high volatility of the condensate and the fact that the temperatures to determine the parameters and to carry out the tests were very lower than the operation temperature. Therefore, a security factor was applied on the predictions (less 20%). (author)

  3. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury.

    Science.gov (United States)

    Bosmann, Markus; Grailer, Jamison J; Ruemmler, Robert; Russkamp, Norman F; Zetoune, Firas S; Sarma, J Vidya; Standiford, Theodore J; Ward, Peter A

    2013-12-01

    We investigated how complement activation promotes tissue injury and organ dysfunction during acute inflammation. Three models of acute lung injury (ALI) induced by LPS, IgG immune complexes, or C5a were used in C57BL/6 mice, all models requiring availability of both C5a receptors (C5aR and C5L2) for full development of ALI. Ligation of C5aR and C5L2 with C5a triggered the appearance of histones (H3 and H4) in bronchoalveolar lavage fluid (BALF). BALF from humans with ALI contained H4 histone. Histones were absent in control BALF from healthy volunteers. In mice with ALI, in vivo neutralization of H4 with IgG antibody reduced the intensity of ALI. Neutrophil depletion in mice with ALI markedly reduced H4 presence in BALF and was highly protective. The direct lung damaging effects of extracellular histones were demonstrated by airway administration of histones into mice and rats (Sprague-Dawley), which resulted in ALI that was C5a receptor-independent, and associated with intense inflammation, PMN accumulation, damage/destruction of alveolar epithelial cells, together with release into lung of cytokines/chemokines. High-resolution magnetic resonance imaging demonstrated lung damage, edema and consolidation in histone-injured lungs. These studies confirm the destructive C5a-dependent effects in lung linked to appearance of extracellular histones.

  4. Geometric Programming Approach to an Interactive Fuzzy Inventory Problem

    Directory of Open Access Journals (Sweden)

    Nirmal Kumar Mandal

    2011-01-01

    Full Text Available An interactive multiobjective fuzzy inventory problem with two resource constraints is presented in this paper. The cost parameters and index parameters, the storage space, the budgetary cost, and the objective and constraint goals are imprecise in nature. These parameters and objective goals are quantified by linear/nonlinear membership functions. A compromise solution is obtained by geometric programming method. If the decision maker is not satisfied with this result, he/she may try to update the current solution to his/her satisfactory solution. In this way we implement man-machine interactive procedure to solve the problem through geometric programming method.

  5. Differences in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages.

    Science.gov (United States)

    Sawai, Hiroaki; Nagashima, Junichi; Kuwahara, Msayasu; Kitagata, Rina; Tamura, Takehiro; Matsui, Ikuo

    2007-09-01

    The pyrimidine bases of RNA are uracil (U) and cytosine (C), while thymine (T) and C are used for DNA. The C(5) position of C and U is unsubstituted, whereas the C(5) of T is substituted with a Me group. Miller et al. hypothesized that various C(5)-substituted uracil derivatives were formed during chemical evolution, and that C(5)-substituted U derivatives may have played important roles in the transition from an 'RNA world' to a 'DNA-RNA-protein world'. Hyperthermophilic bacteria and archaea are considered to be primitive organisms that are evolutionarily close to the universal ancestor of all life on earth. Thus, we examined the substrate specificity of several C(5)-substituted or C(5)-unsubstituted dUTP and dCTP analogs for several DNA polymerases from hyperthermophilic bacteria, hyperthermophilic archaea, and viruses during PCR or primer extension reaction. The substrate specificity of the C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides varied greatly depending on the type of DNA polymerase. The significance of this difference in substrate specificity in terms of the origin and evolution of the DNA replication system is discussed briefly.

  6. Primary complement C5 deficiencies – Molecular characterization and clinical review of two families

    DEFF Research Database (Denmark)

    Schejbel, Lone; Fadnes, Dag; Permin, Henrik

    2013-01-01

    of the Norwegian patient, previously diagnosed as homozygous C5 deficient and suffering four Neisseria infections, an additional case of C5 deficiency was discovered, who had experienced one episode of Neisseria infections. Detailed review of the clinical history of the patients and their healthy relatives did...... infections, which is not covered by the current vaccines. These data support the clinical guidelines for patients treated with C5 inhibitors, who are functional C5 deficient by the treatment....

  7. C5 Extract Induces Apoptosis in B16F10 Murine Melanoma Cells ...

    African Journals Online (AJOL)

    Purpose: To investigate the anti-cancer activities of C5 extract (C5E), a new herbal preparation from Korea, on B16F10 cells. Methods: The anti-proliferative effects of C5E were assessed by culturing B16F10 cells in the presence or absence of C5E. Cell cycle progression was analyzed by PI staining using flow cytometry.

  8. Data of evolutionary structure change: 1C5UA-2OQUA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available A 2OQUA ence>SLQYRSGSSWAHTCG... A 2OQUA ence>PLHCLVNGQYAVHG.../index> 2OQU A 2OQUA ence>MVCAG-GDGVR...1C5UA-2OQUA 1C5U 2OQU A A IVGGYTCGANTVPYQVSLNSG-----YHFCGGSLINSQW...entryIDChain>1C5UA ence>SLNSG-----YHFCGence> EEE ----- EE

  9. Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9

    International Nuclear Information System (INIS)

    Wetsel, R.A.; Lemons, R.S.; Le Beau, M.M.; Barnum, S.R.; Noack, D.; Tack, B.F.

    1988-01-01

    A human C5 clone (pC5HG2) was isolated from a cDNA library constructed from Hep G2 mRNA. he DNA sequence showed that the pC5HG2 insert was comprised of 3309 base pairs of pro-C5 coding sequence and 404 base pairs of 3'-untranslated sequence. The derived amino acid sequence contained the entire coding sequence of the C5 α-chain, the β-α-chain junction region, and 100 amino acids (approximately 50%) of the β-chain. Protein sequences of four C5 tryptic peptides were aligned exactly to this sequence and demonstrated that C5 synthesized and secreted by Hep G2 cells is probably identical with plasma-derived C5. Coding sequence alignment of the human C5 sequences with those of murine C5 indicated that 80% of the nucleotides and 79% of the amino acids were placed identically in the two species. Amino acid sequence alignment of the homologous family members C3, C4, and α 2 -macroglobulin with that of C5 demonstrated 27%, 25%, and 19% identity, respectively. As was found in murine C5, the corresponding thiol ester region of human C5 contained several conserved amino acids, but the critical cysteine and glutamine residues which give rise to the intramolecular thiol ester bond in C3, C4, and α 2 -macroglobulin were absent in C5, having been replaced by serine and alanine, respectively. With the use of a panel of hamster-human somatic cell hybrids, the C5 gene was mapped to human chromosome 9. In situ chromosomal hybridization studies employing metaphase cells further localized the gene to bands 9q32-34, with the largest cluster of grains at 9q34.1

  10. Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice.

    Directory of Open Access Journals (Sweden)

    Danny Gauvreau

    Full Text Available BACKGROUND: Obesity is considered as a systemic chronic low grade inflammation characterized by increased serum pro-inflammatory proteins and accumulation of macrophages within white adipose tissue (WAT of obese patients. C5L2, a 7-transmembrane receptor, serves a dual function, binding the lipogenic hormone acylation stimulating protein (ASP, and C5a, involved in innate immunity. AIM: We evaluated the impact of C5L2 on macrophage infiltration in WAT of wildtype (Ctl and C5L2 knock-out (C5L2(-/- mice over 6, 12 and 24 weeks on a chow diet and moderate diet-induced obesity (DIO conditions. RESULTS: In Ctl mice, WAT C5L2 and C5a receptor mRNA increased (up to 10-fold both over time and with DIO. By contrast, in C5L2(-/-, there was no change in C5aR in WAT. C5L2(-/- mice displayed higher macrophage content in WAT, varying by time, fat depot and diet, associated with altered systemic and WAT cytokine patterns compared to Ctl mice. However, in all cases, the M1 (pro- vs M2 (anti-inflammatory macrophage proportion was unchanged but C5L2(-/- adipose tissue secretome appeared to be more chemoattractant. Moreover, C5L2(-/- mice have increased food intake, increased WAT, and altered WAT lipid gene expression, which is reflected systemically. Furthermore, C5L2(-/- mice have altered glucose/insulin metabolism, adiponectin and insulin signalling gene expression in WAT, which could contribute to development of insulin resistance. CONCLUSION: Disruption of C5L2 increases macrophage presence in WAT, contributing to obesity-associated pathologies, and further supports a dual role of complement in WAT. Understanding this effect of the complement system pathway could contribute to targeting treatment of obesity and its comorbidities.

  11. Complement mediated renal inflammation induced by donor brain death : role of renal C5a-C5aR interaction

    NARCIS (Netherlands)

    van Werkhoven, M. B.; Damman, J.; van Dijk, M. C. R. F.; Daha, M. R.; de Jong, I. J.; Leliveld, A.; Krikke, C.; Leuvenink, H. G.; van Goor, H.; van Son, W. J.; Olinga, P.; Hillebrands, J. -L.; Seelen, M. A. J.

    Kidneys retrieved from brain-dead donors have impaired allograft function after transplantation compared to kidneys from living donors. Donor brain death (BD) triggers inflammatory responses, including both systemic and local complement activation. The mechanism by which systemic activated

  12. Assembly and Regulation of the Membrane Attack Complex Based on Structures of C5b6 and sC5b9

    Directory of Open Access Journals (Sweden)

    Michael A. Hadders

    2012-03-01

    Full Text Available Activation of the complement system results in formation of membrane attack complexes (MACs, pores that disrupt lipid bilayers and lyse bacteria and other pathogens. Here, we present the crystal structure of the first assembly intermediate, C5b6, together with a cryo-electron microscopy reconstruction of a soluble, regulated form of the pore, sC5b9. Cleavage of C5 to C5b results in marked conformational changes, distinct from those observed in the homologous C3-to-C3b transition. C6 captures this conformation, which is preserved in the larger sC5b9 assembly. Together with antibody labeling, these structures reveal that complement components associate through sideways alignment of the central MAC-perforin (MACPF domains, resulting in a C5b6-C7-C8β-C8α-C9 arc. Soluble regulatory proteins below the arc indicate a potential dual mechanism in protection from pore formation. These results provide a structural framework for understanding MAC pore formation and regulation, processes important for fighting infections and preventing complement-mediated tissue damage.

  13. C5a receptor deficiency alters energy utilization and fat storage.

    Directory of Open Access Journals (Sweden)

    Christian Roy

    Full Text Available To investigate the impact of whole body C5a receptor (C5aR deficiency on energy metabolism and fat storage.Male wildtype (WT and C5aR knockout (C5aRKO mice were fed a low fat (CHOW or a high fat high sucrose diet-induced obesity (DIO diet for 14 weeks. Body weight and food intake were measured weekly. Indirect calorimetry, dietary fatload clearance, insulin and glucose tolerance tests were also evaluated. Liver, muscle and adipose tissue mRNA gene expression were measured by RT-PCR.At week one and 12, C5aRKO mice on DIO had increased oxygen consumption. After 12 weeks, although food intake was comparable, C5aRKO mice had lower body weight (-7% CHOW, -12% DIO as well as smaller gonadal (-38% CHOW, -36% DIO and inguinal (-29% CHOW, -30% DIO fat pads than their WT counterparts. Conversely, in WT mice, C5aR was upregulated in DIO vs CHOW diets in gonadal adipose tissue, muscle and liver, while C5L2 mRNA expression was lower in C5aRKO on both diet. Furthermore, blood analysis showed lower plasma triglyceride and non-esterified fatty acid levels in both C5aRKO groups, with faster postprandial triglyceride clearance after a fatload. Additionally, C5aRKO mice showed lower CD36 expression in gonadal and muscle on both diets, while DGAT1 expression was higher in gonadal (CHOW and liver (CHOW and DIO and PPARγ was increased in muscle and liver.These observations point towards a role (either direct or indirect for C5aR in energy expenditure and fat storage, suggesting a dual role for C5aR in metabolism as well as in immunity.

  14. Complement C5a receptor antagonism by protamine and poly-L-Arg on human leukocytes.

    Science.gov (United States)

    Olsen, U B; Selmer, J; Kahl, J U

    1988-01-01

    It is shown that protamine selectively and dose-dependently inhibits complement C5a-induced leukocyte responses such as histamine release from basophils, chemiluminescence and beta-glucuronidase release from neutrophils. Protamine produces parallel rightward displacements of the C5a dose-response curves. The inhibitory capacity of the polypeptide is reversible and disappears following repeated washing of exposed cells. In neutrophils poly-L-Arg similarly and specifically antagonizes C5a-induced chemiluminescence and enzyme release. This polymer alone, however, degranulates basophils and neutrophils, leading to histamine and enzyme release, respectively. It is concluded that on human neutrophils the arginine-rich polycations protamine and poly-L-Arg exhibit a competitive C5a receptor antagonism. In addition, protamine inhibits the C5a receptors on basophils. It is hypothesized that molecular conformations of the arginine-rich polycations might bind reversibly to, and block negatively charged groups at the C5a-receptor sites.

  15. Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex

    DEFF Research Database (Denmark)

    Laursen, Nick Stub; Andersen, Kasper Røjkjær; Braren, Ingke

    2011-01-01

    with a protease subunit (Bb or C2a). We determined the crystal structures of the C3b homologue cobra venom factor (CVF) in complex with C5, and in complex with C5 and the inhibitor SSL7 at 4.3 Å resolution. The structures reveal a parallel two-point attachment between C5 and CVF, where the presence of SSL7 only...... slightly affects the C5-CVF interface, explaining the IgA dependence for SSL7-mediated inhibition of C5 cleavage. CVF functions as a relatively rigid binding scaffold inducing a conformational change in C5, which positions its cleavage site in proximity to the serine protease Bb. A general model...

  16. Radioimmunoelectrophoresis, a sensitive method for detecting cleavage of the fifth component of human complement (C5)

    International Nuclear Information System (INIS)

    Perez, H.D.; Ong, R.; Banda, D.; Goldstein, I.M.

    1983-01-01

    A method has been developed for detecting cleavage of human C5 in serum and whole blood as a consequence of complement activation. Standard, single-dimension immunoelectrophoresis was performed using as antibody a radioiodinated IgG fraction prepared from a commercially available antiserum to human C5. Autoradiographs developed after radioimmunoelectrophoresis of either normal human serum or functionally pure human C5 revealed only one precipitin band. In contrast, when either zymosan-treated serum or trypsin-treated human C5 were examined with this technique, two additional precipitin bands were detected. One migrated more anodally than native C5 while the other remained at the origin (cathode). Radioimmunoelectrophoresis was significantly more sensitive as an indicator of complement activation in human serum than either measurements of total hemolytic complement or a standard assay for complement (C5)-derived chemotactic activity. (Auth.)

  17. Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex

    DEFF Research Database (Denmark)

    Laursen, Nick Stub; Andersen, Kasper Røjkjær; Braren, Ingke

    2011-01-01

    with a protease subunit (Bb or C2a). We determined the crystal structures of the C3b homologue cobra venom factor (CVF) in complex with C5, and in complex with C5 and the inhibitor SSL7 at 4.3 Å resolution. The structures reveal a parallel two-point attachment between C5 and CVF, where the presence of SSL7 only...

  18. Adjacent-Vertex-Distinguishing Total Chromatic Numbers of C5 ∨ Kt%图C5∨Kt的邻点可区别全色数

    Institute of Scientific and Technical Information of China (English)

    张芳红; 王治文; 陈祥恩

    2012-01-01

    设f是图G的一个正常全染色.对任意x∈V(G),令C(x)表示与点x相关联的边的颜色以及点x的颜色所构成的集合.若对任意uv∈E(G),有C(u)≠C(v),则称f是图G的一个邻点可区别全染色.对一个图G进行邻点可区别全染色所需的最少的颜色的数目称为G的邻点可区别全色数,记为xat(G).用C5∨Kt表示长为5的圈与t阶完全图的联图.讨论了c5∨Kt的邻点可区别全色数.利用正多边形的对称性构造染色以及组合分析的方法,得到了当t是大于等于3的奇数以及t是偶数且2≤t≤22时,xat(C5∨Kt)=t+6,当t是偶数且t≥24时,Xat(C5∨Kt)=t+7.%Let / be a proper total coloring of G. For each x ∈V(G),let C(x) denote the set of all colors of the edhes incident with x and the color of x. If (V) uv ∈E(G),we have C(u) ≠ C(v),then / is called an adjacent vertex distinguishing total coloring of G. The minimum number k for which there exists an adjacent vertex distinguishing total coloring of G using k colors is called the adjacent vertex distinguishing total chromatic number of G and denoted by Xat(G). Let C5 V Kt be the join of the cycle C5 of order 5 and the complete graph Kt of order t. In this paper,we discuss adjacent-vertex-distinguishing total chromatic numbers of C5 V Kt. By using symmetry of regular polygons to construct coloring,and methods of combinatorial analysis,we obtained that for t is odd with t ≥ 3 or t is even with 2 ≤ t ≤ 22,we have Xat(C5,V Kt) = t + 6; for t is even with t ≥ 24,we have Xat(C5 V Kt) = t + 7.

  19. Experimental Malaria in Pregnancy Induces Neurocognitive Injury in Uninfected Offspring via a C5a-C5a Receptor Dependent Pathway.

    Directory of Open Access Journals (Sweden)

    Chloë R McDonald

    2015-09-01

    Full Text Available The in utero environment profoundly impacts childhood neurodevelopment and behaviour. A substantial proportion of pregnancies in Africa are at risk of malaria in pregnancy (MIP however the impact of in utero exposure to MIP on fetal neurodevelopment is unknown. Complement activation, in particular C5a, may contribute to neuropathology and adverse outcomes during MIP. We used an experimental model of MIP and standardized neurocognitive testing, MRI, micro-CT and HPLC analysis of neurotransmitter levels, to test the hypothesis that in utero exposure to malaria alters neurodevelopment through a C5a-C5aR dependent pathway. We show that malaria-exposed offspring have persistent neurocognitive deficits in memory and affective-like behaviour compared to unexposed controls. These deficits were associated with reduced regional brain levels of major biogenic amines and BDNF that were rescued by disruption of C5a-C5aR signaling using genetic and functional approaches. Our results demonstrate that experimental MIP induces neurocognitive deficits in offspring and suggest novel targets for intervention.

  20. C5a receptor (CD88) blockade protects against MPO-ANCA GN.

    Science.gov (United States)

    Xiao, Hong; Dairaghi, Daniel J; Powers, Jay P; Ertl, Linda S; Baumgart, Trageen; Wang, Yu; Seitz, Lisa C; Penfold, Mark E T; Gan, Lin; Hu, Peiqi; Lu, Bao; Gerard, Norma P; Gerard, Craig; Schall, Thomas J; Jaen, Juan C; Falk, Ronald J; Jennette, J Charles

    2014-02-01

    Necrotizing and crescentic GN (NCGN) with a paucity of glomerular immunoglobulin deposits is associated with ANCA. The most common ANCA target antigens are myeloperoxidase (MPO) and proteinase 3. In a manner that requires activation of the alternative complement pathway, passive transfer of antibodies to mouse MPO (anti-MPO) induces a mouse model of ANCA NCGN that closely mimics human disease. Here, we confirm the importance of C5aR/CD88 in the mediation of anti-MPO-induced NCGN and report that C6 is not required. We further demonstrate that deficiency of C5a-like receptor (C5L2) has the reverse effect of C5aR/CD88 deficiency and results in more severe disease, indicating that C5aR/CD88 engagement enhances inflammation and C5L2 engagement suppresses inflammation. Oral administration of CCX168, a small molecule antagonist of human C5aR/CD88, ameliorated anti-MPO-induced NCGN in mice expressing human C5aR/CD88. These observations suggest that blockade of C5aR/CD88 might have therapeutic benefit in patients with ANCA-associated vasculitis and GN.

  1. Multimodal interaction in image and video applications

    CERN Document Server

    Sappa, Angel D

    2013-01-01

    Traditional Pattern Recognition (PR) and Computer Vision (CV) technologies have mainly focused on full automation, even though full automation often proves elusive or unnatural in many applications, where the technology is expected to assist rather than replace the human agents. However, not all the problems can be automatically solved being the human interaction the only way to tackle those applications. Recently, multimodal human interaction has become an important field of increasing interest in the research community. Advanced man-machine interfaces with high cognitive capabilities are a hot research topic that aims at solving challenging problems in image and video applications. Actually, the idea of computer interactive systems was already proposed on the early stages of computer science. Nowadays, the ubiquity of image sensors together with the ever-increasing computing performance has open new and challenging opportunities for research in multimodal human interaction. This book aims to show how existi...

  2. The complement anaphylatoxin C5a receptor contributes to obese adipose tissue inflammation and insulin resistance.

    Science.gov (United States)

    Phieler, Julia; Chung, Kyoung-Jin; Chatzigeorgiou, Antonios; Klotzsche-von Ameln, Anne; Garcia-Martin, Ruben; Sprott, David; Moisidou, Maria; Tzanavari, Theodora; Ludwig, Barbara; Baraban, Elena; Ehrhart-Bornstein, Monika; Bornstein, Stefan R; Mziaut, Hassan; Solimena, Michele; Karalis, Katia P; Economopoulou, Matina; Lambris, John D; Chavakis, Triantafyllos

    2013-10-15

    Obese adipose tissue (AT) inflammation contributes critically to development of insulin resistance. The complement anaphylatoxin C5a receptor (C5aR) has been implicated in inflammatory processes and as regulator of macrophage activation and polarization. However, the role of C5aR in obesity and AT inflammation has not been addressed. We engaged the model of diet-induced obesity and found that expression of C5aR was significantly upregulated in the obese AT, compared with lean AT. In addition, C5a was present in obese AT in the proximity of macrophage-rich crownlike structures. C5aR-sufficient and -deficient mice were fed a high-fat diet (HFD) or a normal diet (ND). C5aR deficiency was associated with increased AT weight upon ND feeding in males, but not in females, and with increased adipocyte size upon ND and HFD conditions in males. However, obese C5aR(-/-) mice displayed improved systemic and AT insulin sensitivity. Improved AT insulin sensitivity in C5aR(-/-) mice was associated with reduced accumulation of total and proinflammatory M1 macrophages in the obese AT, increased expression of IL-10, and decreased AT fibrosis. In contrast, no difference in β cell mass was observed owing to C5aR deficiency under an HFD. These results suggest that C5aR contributes to macrophage accumulation and M1 polarization in the obese AT and thereby to AT dysfunction and development of AT insulin resistance.

  3. The (gas + liquid) critical properties and phase behaviour of some binary alkanol (C2-C5) + alkane (C5-C12) mixtures

    International Nuclear Information System (INIS)

    Morton, David W.; Lui, Matthew P.W.; Young, Colin L.

    2003-01-01

    Previously, the investigation of the (gas + liquid) critical properties of (alkanol + alkane) mixtures has focussed on (primary alkanol + straight chain alkane) mixtures. The experimental data available for (alkanol + alkane) mixtures, which include secondary or tertiary alcohols and/or branched chain alkanes, are extremely limited. This work extends the existing body of data on (alkanol + alkane) mixtures to include mixtures containing these components. Here the (gas + liquid) critical temperatures of 29 {alkanol (C 2 -C 5 ) + alkane (C 5 -C 12 )} mixtures are reported. All the (gas + liquid) critical lines for the binary mixtures studied are continuous, indicating they obey either Type I or Type II phase behaviour

  4. Critical role for complement receptor C5aR2 in the pathogenesis of renal ischemia-reperfusion injury

    NARCIS (Netherlands)

    Poppelaars, Felix; van Werkhoven, Maaike B; Kotimaa, Juha; Veldhuis, Zwanida J; Ausema, Albertina; Broeren, Stefan G M; Damman, Jeffrey; Hempel, Julia C.; Leuvenink, Henri G D; Daha, Mohamed R; van Son, Willem J; van Kooten, Cees; van Os, Ronald P; Hillebrands, Jan-Luuk; Seelen, Marc A

    The complement system, and specifically C5a, is involved in renal ischemia-reperfusion (IR) injury. The 2 receptors for complement anaphylatoxin C5a (C5aR1 and C5aR2) are expressed on leukocytes as well as on renal epithelium. Extensive evidence shows that C5aR1 inhibition protects kidneys from IR

  5. C5a binding to human polymorphonuclear leukocyte plasma membrane (PMNLM) receptors

    International Nuclear Information System (INIS)

    Conway, R.G.; Mollison, K.W.; Carter, G.W.; Lane, B.

    1986-01-01

    Previous investigations of the C5a receptor have been performed using intact human PMNL. To circumvent some of the potential problems with such whole cell assays (e.g. internalization or metabolism of radioligand) the authors have developed a PMNLM binding assay. Human PMNLM were prepared by nitrogen cavitation and Percoll gradient centrifugation. Specific binding of [ 125 I]C5a to PMNLM was: high affinity, K/sub D/ = 0.6 nM; saturable, B/sub max/ = 8.7 pmol/mg protein; and reversible. Kinetic measurements agree with the K/sub D/ value obtained by Scatchard analysis. Furthermore, the binding activity of C5a correlates with biological activity as measured by myeloperoxidase release from human PMNL. Human serum C5a and recombinant C5a bind with similar affinities when measured by competition or direct binding and label the same number of sites in human PMNLM. The nonhydrolyzable GTP analog, GppNHp, induces a low affinity state of the C5a receptor (4-6 fold shift in K/sub D/) with little effect on B/sub max/. In summary, the criteria have been satisfied for identification of a biologically relevant C5a binding site in human PMNLM. Regulation of the C5a receptor and its membrane transduction mechanism(s) appears to involve guanyl nucleotides, as has been found for other chemoattractant receptors

  6. Heterogeneity of d-glucuronyl C5-epimerase expression and epigenetic regulation in prostate cancer

    International Nuclear Information System (INIS)

    Prudnikova, Tatiana Y; Soulitzis, Nikolaos; Kutsenko, Olesya S; Mostovich, Lyudmila A; Haraldson, Klas; Ernberg, Ingemar; Kashuba, Vladimir I; Spandidos, Demetrios A; Zabarovsky, Eugene R; Grigorieva, Elvira V

    2013-01-01

    Heparansulfate proteoglycans (HSPG) play an important role in cell–cell and cell–matrix interactions and signaling, and one of the key enzymes in heparansulfate biosynthesis is d-glucuronyl C5-epimerase (GLCE). A tumor suppressor function has been demonstrated for GLCE in breast and lung carcinogenesis; however, no data are available as to the expression and regulation of the gene in prostate cancer. In this study, decreased GLCE expression was observed in 10% of benign prostate hyperplasia (BPH) tissues and 53% of prostate tumors, and increased GLCE mRNA levels were detected in 49% of BPH tissues and 21% of tumors. Statistical analysis showed a positive correlation between increased GLCE expression and Gleason score, TNM staging, and prostate-specific antigen (PSA) level in the prostate tumors (Pearson correlation coefficients GLCE/Gleason = 0.56, P < 0.05; GLCE/TNM = 0.62, P < 0.05; and GLCE/PSA = 0.88, P < 0.01), suggesting GLCE as a candidate molecular marker for advanced prostate cancer. Immunohistochemical analysis revealed an intratumoral heterogeneity of GLCE protein levels both in BPH and prostate cancer cells, resulting in a mixed population of GLCE-expressing and nonexpressing epithelial cells in vivo. A model experiment on normal (PNT2) and prostate cancer (LNCaP, PC3, DU145) cell lines in vitro showed a 1.5- to 2.5-fold difference in GLCE expression levels between the cancer cell lines and an overall decrease in GLCE expression in cancer cells. Methyl-specific polymerase chain reaction (PCR), bisulfite sequencing, and deoxy-azacytidin (aza-dC) treatment identified differential GLCE promoter methylation (LNCaP 70–72%, PC3 32–35%, DU145, and PNT2 no methylation), which seems to contribute to heterogeneous GLCE expression in prostate tumors. The obtained results reveal the complex deregulation of GLCE expression in prostatic diseases compared with normal prostate tissue and suggest that GLCE may be used as a potential model to study the functional

  7. NMR and TRLFS studies of Ln(iii) and An(iii) C5-BPP complexes.

    Science.gov (United States)

    Adam, Christian; Beele, Björn B; Geist, Andreas; Müllich, Udo; Kaden, Peter; Panak, Petra J

    2015-02-01

    C5-BPP is a highly efficient N-donor ligand for the separation of trivalent actinides, An(iii), from trivalent lanthanides, Ln(iii). The molecular origin of the selectivity of C5-BPP and many other N-donor ligands of the BTP-type is still not entirely understood. We present here the first NMR studies on C5-BPP Ln(iii) and An(iii) complexes. C5-BPP is synthesized with 10% 15 N labeling and characterized by NMR and LIFDI-MS methods. 15 N NMR spectroscopy gives a detailed insight into the bonding of C5-BPP with lanthanides and Am(iii) as a representative for trivalent actinide cations, revealing significant differences in 15 N chemical shift for coordinating nitrogen atoms compared to Ln(iii) complexes. The temperature dependence of NMR chemical shifts observed for the Am(iii) complex indicates a weak paramagnetism. This as well as the observed large chemical shift for coordinating nitrogen atoms show that metal-ligand bonding in Am(C5-BPP) 3 has a larger share of covalence than in lanthanide complexes, confirming earlier studies. The Am(C5-BPP) 3 NMR sample is furthermore spiked with Cm(iii) and characterized by time-resolved laser fluorescence spectroscopy (TRLFS), yielding important information on the speciation of trace amounts of minor complex species.

  8. Receptor residence time trumps drug-likeness and oral bioavailability in determining efficacy of complement C5a antagonists

    Science.gov (United States)

    Seow, Vernon; Lim, Junxian; Cotterell, Adam J.; Yau, Mei-Kwan; Xu, Weijun; Lohman, Rink-Jan; Kok, W. Mei; Stoermer, Martin J.; Sweet, Matthew J.; Reid, Robert C.; Suen, Jacky Y.; Fairlie, David P.

    2016-04-01

    Drug discovery and translation are normally based on optimizing efficacy by increasing receptor affinity, functional potency, drug-likeness (rule-of-five compliance) and oral bioavailability. Here we demonstrate that residence time of a compound on its receptor has an overriding influence on efficacy, exemplified for antagonists of inflammatory protein complement C5a that activates immune cells and promotes disease. Three equipotent antagonists (3D53, W54011, JJ47) of inflammatory responses to C5a (3nM) were compared for drug-likeness, receptor affinity and antagonist potency in human macrophages, and anti-inflammatory efficacy in rats. Only the least drug-like antagonist (3D53) maintained potency in cells against higher C5a concentrations and had a much longer duration of action (t1/2 ~ 20 h) than W54011 or JJ47 (t1/2 ~ 1-3 h) in inhibiting macrophage responses. The unusually long residence time of 3D53 on its receptor was mechanistically probed by molecular dynamics simulations, which revealed long-lasting interactions that trap the antagonist within the receptor. Despite negligible oral bioavailability, 3D53 was much more orally efficacious than W54011 or JJ47 in preventing repeated agonist insults to induce rat paw oedema over 24 h. Thus, residence time on a receptor can trump drug-likeness in determining efficacy, even oral efficacy, of pharmacological agents.

  9. CLONING, PURIFICATION AND CHARACTERIZATION OF HALOTOLERANT XYLANASE FROM Geobacillus Thermodenitrificans C5

    Directory of Open Access Journals (Sweden)

    Muhammad Irfan

    2016-06-01

    Full Text Available High levels of extracellular xylanase activity (994.50 IU/ml produced by Geobacillus thermodenitrificans C5 originated gene was detected when it was expressed in E. coli BL21 host. Thermostable xylanase (GthC5Xyl was purified to homogeneity and showed a molecular mass of approximately 44 kDa according to SDS-PAGE. The specific activity of the purified GthC5Xyl was up to 1243.125IU/mg with a 9.89-fold purification. The activity of GthC5Xyl was stimulated by CoCl2, MnSO4, CuSO4, MnCl2 but was inhibited by FeSO4, Hg, CaSO4. GthC5Xyl showed resistant to SDS, Tween 20, Triton X-100, β- Mercaptoethanol, PMSF, DTT, NEM and DEPC, SDS, and EDTA. A greater affinity for oat spelt xylan was exhibited by GthC5Xyl with maximum enzymatic activity at 60°C and 6.0 pH. The activity portrayed by GthC5Xyl was found to be acellulytic with stability at high temperature (70°C-80°C and low pH (4.0 to 8.0. Xylobiose and xylopentose were the end products of proficient oat spelt xylanase hydrolysis by GthC5Xyl. High SDS resistance and broader stability of GthC5Xyl proves it to be worthy of impending application in numerous industrial processes especially textile, detergents and animal feed industry.

  10. Estrogen enhances expression of the complement C5a receptor and the C5a-agonist evoked calcium influx in hormone secreting neurons of the hypothalamus.

    Science.gov (United States)

    Farkas, Imre; Varju, Patricia; Szabo, Emese; Hrabovszky, Erik; Okada, Noriko; Okada, Hidechika; Liposits, Zsolt

    2008-01-01

    In the present study we examined presence of the complement C5a receptor (C5aR) in hypothalamic neurosecretory neurons of the rodent brain and effect of estrogen on C5aR expression. Whole cell patch clamp measurements revealed that magnocellular neurons in the supraoptic and paraventricular nuclei of hypothalamic slices of the rats responded to the C5aR-agonist PL37-MAP peptide with calcium ion current pulses. Gonadotropin-releasing hormone (GnRH) producing neurons in slices of the preoptic area of the mice also reacted to the peptide treatment with inward calcium current. PL37-MAP was able to evoke the inward ion current of GnRH neurons in slices from ovariectomized animals. The amplitude of the inward pulses became higher in slices obtained from 17beta-estradiol (E2) substituted mice. Calcium imaging experiments demonstrated that PL37-MAP increased the intracellular calcium content in the culture of the GnRH-producing GT1-7 cell line in a concentration-dependent manner. Calcium imaging also showed that E2 pretreatment elevated the PL37-MAP evoked increase of the intracellular calcium content in the GT1-7 cells. The estrogen receptor blocker Faslodex in the medium prevented the E2-evoked increase of the PL37-MAP-triggered elevation of the intracellular calcium content in the GT1-7 cells demonstrating that the effect of E2 might be related to the presence of estrogen receptor. Real-time PCR experiments revealed that E2 increased the expression of C5aR mRNA in GT1-7 neurons, suggesting that an increased C5aR synthesis could be involved in the estrogenic modulation of calcium response. These data indicate that hypothalamic neuroendocrine neurons can integrate immune and neuroendocrine functions. Our results may serve a better understanding of the inflammatory and neurodegeneratory diseases of the hypothalamus and the related neuroendocrine and autonomic compensatory responses.

  11. A new man-machine-interface at BESSY

    International Nuclear Information System (INIS)

    Mueller, R.; Doll, H.D.; Donasch, I.J.; Marxen, H.; Pause, H.

    1991-01-01

    A UIMS (user interface management system) has been developed, that is completely based on non-proprietary software. Central part of the UIMS are processes (mapper) that act as universal X-clients for each specified X-server. Mapper (graphic server) and applications (graphic clients) exchange requests by an event driven interface. The communication protocol is free from any graphical information. The most powerful mapper client is a form interpreter, that can be programmed to act as an equipment access server. Mapper and form interpreter allow to compose control panels and synoptic views of the machine with statements in a simple and comprehensible UIDL (user interface definition language)

  12. Using cognitive modeling to improve the man-machine interface

    International Nuclear Information System (INIS)

    Newton, R.A.; Zyduck, R.C.; Johnson, D.R.

    1982-01-01

    A group of utilities from the Westinghouse Owners Group was formed in early 1980 to examine the interface requirements and to determine how they could be implemented. The products available from the major vendors were examined early in 1980 and judged not to be completely applicable. The utility group then decided to develop its own specifications for a Safety Assessment System (SAS) and, later in 1980, contracted with a company to develop the system, prepare the software and demonstrate the system on a simulator. The resulting SAS is a state-of-the-art system targeted for implementation on pressurized water reactor nuclear units. It has been designed to provide control room operators with centralized and easily understandable information from a computer-based data and display system. This paper gives an overview of the SAS plus a detailed description of one of its functional areas - called AIDS. The AIDS portion of SAS is an advanced concept which uses cognitive modeling of the operator as the basis for its design

  13. Man-machine interface: Description of recent developments by manufacturers

    International Nuclear Information System (INIS)

    Hessler, C.

    1995-01-01

    Two examples have been chosen (Staudinger NPP, unit 5, and Chooz B NPP) for illustrating characteristic features of screen-based control rooms in operation. Future development trends are: harmonisation and standardisation of hardware and software; integration of process control and operation control functions; novel visualisation concepts. The most visible progress in hardware development for future screen-based control rooms is the large-size screens for control rooms in addition to the smaller PC screens, which offer to the entire control room crew a full-scale view of plant behaviour at a glance. The next, logical advancements to come are for improved visualisation, as e.g. dynamic, coloured display of plant systems, or more abstract schematic, dynamic display of flowsheets of media and signals for information of operators, enhanced by pop-up video pictures of systems if requested. Integration of electronic operator manuals is realised to some extent and is continued, giving guidance on incident analysis supported by on-screen display, for accident management in NPP. The above tools will be installed in the control room of the EPR. The concept is to install redundant information tools, the system for every operator at the workplace, and a large-size system showing the entire plant systems for common information and coordination of operator response. Common-mode failures are planned to be prevented by complementary systems of a higher safety-engineering class. (orig./HP) [de

  14. General man-machine interface used in accelerators controls

    International Nuclear Information System (INIS)

    Boutheon, M.; Di Maio, F.; Pace, A.

    1992-01-01

    A large community is now using Workstations as Accelerators Computer Controls Interface, through the concepts of windows - menus - synoptics - icons. Some standards were established for the CERN-PS control systems rejuvenation. The Booster-to-PS transfer and injection process is now entirely operated with these tools. This application constitutes a global environment providing the users with the controls, analysis, visualization of a part of an accelerator. Individual commands, measurements, and specialized programs including complex treatments are available in a homogeneous frame. Some months of experience in current operation have shown that this model can be extended to the whole project. (author)

  15. Affective Man-Machine Interface: Unveiling human emotions through biosignals

    NARCIS (Netherlands)

    van den Broek, Egon; Lisy, Viliam; Janssen, Joris H.; Westerink, Joyce H.D.M.; Schut, Marleen H.; Tuinenbreijer, Kees; Fred, A.; Filipe, J.; Gamboa, H.

    2010-01-01

    As is known for centuries, humans exhibit an electrical profile. This profile is altered through various psychological and physiological processes, which can be measured through biosignals; e.g., electromyography (EMG) and electrodermal activity (EDA). These biosignals can reveal our emotions and,

  16. Advanced Avionics and the Military Aircraft Man/Machine Interface.

    Science.gov (United States)

    1982-07-01

    technItrqtiJ pour d V,’II ’] ITIOU V eau Ut retentis,ement phys1i0l,ZiqUe. iettpor~ CeLt (qUC OUS Seron, trnenes ioarfoi, a evcquer cet aspect te’chnique... traitement en plusleurs 6tapes -L’acquisition du signal acoustique, et sa num~risation apr~s passage dans un banc de filtres on obtient ainsi un sonagraune

  17. Man-machine enhancements to existing FFTF control panels

    International Nuclear Information System (INIS)

    Miller, E.M.

    1984-01-01

    FFTF Project enhanced existing control panels with tape and labels to mitigate operator problems and to incorporate the guidance of NUREG-0700. The enhancements grouped displays and controls into meaningful units and labelled controls and displays to facilitate their identification and efficient use. The enhancements were inexpensive and well received by the facility's operations staff

  18. Transient Classifier Systems and Man-Machine Interface Research.

    Science.gov (United States)

    1987-08-31

    different timbre from two different resonant sources, i.e., like a violin and oboe emitting nearly the same fundamental mode fre- quency, but each with its...the subjects by examing both hits and misses for signal and noise stimuli. A pairwise com- parison of the means resulted in significant differences (at

  19. Evaluation of man-machine systems - methods and problems

    International Nuclear Information System (INIS)

    1985-01-01

    The symposium gives a survey of the methods of evaluation which permit as quantitive an assessment as possible of the collaboration between men and machines. This complex of problems is of great current significance in many areas of application. The systems to be evaluated are aircraft, land vehicles and watercraft as well as process control systems. (orig./GL) [de

  20. Make man-machine communication easier: fuzzy programming

    Energy Technology Data Exchange (ETDEWEB)

    Farreny, H; Prade, H

    1982-06-01

    Procedures and data used by the human brain are not always accurately specified; fuzzy programming may help in the realisation of languages for the manipulation of such fuzzy entities. After having considered fuzzy instruction and its requirements, arguments, functions, predicates and designations, the authors present the outlines of a fuzzy filtering system. Two applications are given as examples; these are the accessing of a database and an expert system which may be used to solve problems in robotics.

  1. Touch screen man machine interfere for emergency dose calculations

    International Nuclear Information System (INIS)

    Woodard, K.; Abrams, M.

    1987-01-01

    Emergency dose calculation systems generally use a keyboard to provide the interface between the user and the computer. This interface is preferred by users who work daily with computers; however, for many plant personnel who are not continuously involved with computer operations, the use of a keyboard can be cumbersome and time consuming. This is particularly true when the user is under pressure during a drill or an actual emergency. Experience in many applications of Pickard, Lowe and Garrick's PLG's Meteorological Information and Dose Assessment System (MIDAS) has shown that user friendliness is a key ingredient toward achieving acceptance of computerized systems. Hardware to support to touch screen interface is now available and has been implemented in MIDAS. Recent experience has demonstrated that selection times for dose calculations are reduced, data entry errors have been minimized, and confusion over appropriate entries has been avoided due to the built-in logic. A 10-yr search for an acceptable keyboard replacement has ended

  2. Optimization of the man-machine interface for LMFBRs

    International Nuclear Information System (INIS)

    Seeman, S.E.; Colley, R.W.; Stratton, R.C.

    1982-01-01

    An effort is underway to optimize the roles of man and machine in control of Liquid Metal Fast Breeder Reactors. The work reported on here describes two systems that have been developed. The first of these, MIDAS, is a large data base system developed for use at FFTF as an aid to operators in determining how to proceed with maintenance and repairs to be carried out on plant components. This system is presently in use at FFTF. The second system, the Procedure Prompting System, is a system being developed to demonstrate a new methodology for automatically generating off-normal plant recovery instructions. The methodology for this system has been demonstrated on a model of a small subsystem of FFTF

  3. New man-machine interfaces in nuclear power plants

    International Nuclear Information System (INIS)

    1994-01-01

    Taking into account the acknowledgment that human factors significantly contribute to abnormal events during nuclear power plants operation, this report presents new trends in control rooms human factors engineering. International experience is given in finding a proper balance between data richness and keeping an uppermost vigilance. Alarm computerization and expert systems are speeding, but no certification procedure is able to evaluate their reliability. (D.L.). 13 refs., figs., tabs

  4. Manipulator system man-machine interface evaluation program. [technology assessment

    Science.gov (United States)

    Malone, T. B.; Kirkpatrick, M.; Shields, N. L.

    1974-01-01

    Application and requirements for remote manipulator systems for future space missions were investigated. A manipulator evaluation program was established to study the effects of various systems parameters on operator performance of tasks necessary for remotely manned missions. The program and laboratory facilities are described. Evaluation criteria and philosophy are discussed.

  5. Analysis of Pacific Enroute Structure in Support of C-5M Super Galaxy

    Science.gov (United States)

    2015-06-19

    Storyboard ....................................................................................................43 Appendix B. Travis AFB C-5...newest strategic airlifter can be both effective for the combatant commander and efficient for the taxpayer. 42 Appendix A. Storyboard 43

  6. THE DECEPTIVELY SIMPLE THERMOLYSIS OF TRIVALENT PERMETHYLTITANOCENE DERIVATIVES (ETA-5-C5ME5)2TIR - FORMATION OF A TETRAMETHYLFULVENE TITANIUM COMPOUND (ETA-6-C5ME4CH2)(ETA-5-C5ME5)TI AND RH, CATALYZED BY PERMETHYLTITANOCENE HYDRIDE, (ETA-5-C5ME5)2TIH

    NARCIS (Netherlands)

    LUINSTRA, GA; TEUBEN, JH

    1992-01-01

    The complexes Cp*2TiR (Cp* = eta-5-C5Me5; R = Me, Et, n-Pr, C2H3, CH2CMe3, Ph) undergo thermolysis to yield the fulvene complex Cp*FvTi (Fv = eta-6-C5Me4CH2) and RH. Kinetic measurements and deuterium labeling studies show that the decomposition is catalyzed by Cp*2TiH, which is formed either by

  7. The Deceptively Simple Thermolysis of Trivalent Permethyltitanocene Derivatives (η5-C5Me5)2TiR. Formation of a Tetramethylfulvene Titanium Compound (η6-C5Me4CH2)(η5-C5Me5)Ti and RH, Catalyzed by Permethyltitanocene Hydride, (η5-C5Me5)2TiH

    NARCIS (Netherlands)

    Luinstra, Gerrit A.; Teuben, Jan H.

    1992-01-01

    The complexes Cp*2TiR (Cp* = η5-C5Me5; R = Me, Et, n-Pr, C2H3, CH2CMe3, Ph) undergo thermolysis to yield the fulvene complex Cp*FvTi (Fv = η6-C5Me4CH2) and RH. Kinetic measurements and deuterium labeling studies show that the decomposition is catalyzed by Cp*2TiH, which is formed either by

  8. The upgraded control and instrumentation system of C5 irradiation device

    International Nuclear Information System (INIS)

    Iordache, A.; Ancuta, M.; Gruia, L.; Pulpa, A.; Salistean, E.; Gusescu, G.

    2013-01-01

    C5 capsule is an irradiation device of TRIGA SSR, which is designed for irradiation of structural materials in an inert environment for mechanical behavior characterization and the material microstructure evolution during irradiation. The paper presents the upgraded control and instrumentation system of the C5 irradiation device which was designed and manufactured to enhance the performance of this system for better surveillance and processing the acquired experimental data. (authors)

  9. Man-Man-Machine-Machine Etiquette: Towards an Environment to Investigate Behavioral Rules for Dynamic Man-Machine Teams

    NARCIS (Netherlands)

    Doesburg, W.A. van; Heuvelink, A.; Langeslag, P.; Kraker, de K.J.

    2010-01-01

    In order for a defense organization to gain understanding of their performance in complex tactical situations it is important to accurately model the way opposing and own forces operate. It is a significant challenge to develop such models due to the increasing interdependence between personnel and

  10. Hybrid DNA i-motif: Aminoethylprolyl-PNA (pC5) enhance the stability of DNA (dC5) i-motif structure.

    Science.gov (United States)

    Gade, Chandrasekhar Reddy; Sharma, Nagendra K

    2017-12-15

    This report describes the synthesis of C-rich sequence, cytosine pentamer, of aep-PNA and its biophysical studies for the formation of hybrid DNA:aep-PNAi-motif structure with DNA cytosine pentamer (dC 5 ) under acidic pH conditions. Herein, the CD/UV/NMR/ESI-Mass studies strongly support the formation of stable hybrid DNA i-motif structure with aep-PNA even near acidic conditions. Hence aep-PNA C-rich sequence cytosine could be considered as potential DNA i-motif stabilizing agents in vivo conditions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. C5 Palsy After Cervical Spine Surgery: A Multicenter Retrospective Review of 59 Cases.

    Science.gov (United States)

    Thompson, Sara E; Smith, Zachary A; Hsu, Wellington K; Nassr, Ahmad; Mroz, Thomas E; Fish, David E; Wang, Jeffrey C; Fehlings, Michael G; Tannoury, Chadi A; Tannoury, Tony; Tortolani, P Justin; Traynelis, Vincent C; Gokaslan, Ziya; Hilibrand, Alan S; Isaacs, Robert E; Mummaneni, Praveen V; Chou, Dean; Qureshi, Sheeraz A; Cho, Samuel K; Baird, Evan O; Sasso, Rick C; Arnold, Paul M; Buser, Zorica; Bydon, Mohamad; Clarke, Michelle J; De Giacomo, Anthony F; Derakhshan, Adeeb; Jobse, Bruce; Lord, Elizabeth L; Lubelski, Daniel; Massicotte, Eric M; Steinmetz, Michael P; Smith, Gabriel A; Pace, Jonathan; Corriveau, Mark; Lee, Sungho; Cha, Peter I; Chatterjee, Dhananjay; Gee, Erica L; Mayer, Erik N; McBride, Owen J; Roe, Allison K; Yanez, Marisa Y; Stroh, D Alex; Than, Khoi D; Riew, K Daniel

    2017-04-01

    A multicenter, retrospective review of C5 palsy after cervical spine surgery. Postoperative C5 palsy is a known complication of cervical decompressive spinal surgery. The goal of this study was to review the incidence, patient characteristics, and outcome of C5 palsy in patients undergoing cervical spine surgery. We conducted a multicenter, retrospective review of 13 946 patients across 21 centers who received cervical spine surgery (levels C2 to C7) between January 1, 2005, and December 31, 2011, inclusive. P values were calculated using 2-sample t test for continuous variables and χ 2 tests or Fisher exact tests for categorical variables. Of the 13 946 cases reviewed, 59 patients experienced a postoperative C5 palsy. The incidence rate across the 21 sites ranged from 0% to 2.5%. At most recent follow-up, 32 patients reported complete resolution of symptoms (54.2%), 15 had symptoms resolve with residual effects (25.4%), 10 patients did not recover (17.0%), and 2 were lost to follow-up (3.4%). C5 palsy occurred in all surgical approaches and across a variety of diagnoses. The majority of patients had full recovery or recovery with residual effects. This study represents the largest series of North American patients reviewed to date.

  12. C5b-9 Staining Correlates With Clinical and Tumor Stage in Gastric Adenocarcinoma.

    Science.gov (United States)

    Chen, Jian; Yang, Wei-Jun; Sun, Hai-Jian; Yang, Xia; Wu, Yu-Zhang

    2016-08-01

    The complement system is a critical part of the immune response, acting in defense against viral infections, clearance of immune complexes, and maintenance of tissue homeostasis. Upregulated expression of the terminal complement complex, C5b-9, has been observed on various tumor cells, such as stomach carcinoma cells, and on cells in the necrotic regions of these tumors as well; however, whether and how C5b-9 is related to gastric cancer progression and severity remains unknown. In this study, human gastric adenocarcinoma (HGAC) tissues (n=47 cases) and patient-matched adjacent nontumoral parenchyma (n=20 cases) were evaluated by tissue microarray and immunohistochemistry. The HGAC tissues showed upregulated C5b-9 expression. Multinomial logistic regression and likelihood ratio testing showed that overexpression of C5b-9 in HGAC tissue was significantly correlated with clinical stage (P=0.007) and tumor stage (P=0.005), but not with tumor distant organ metastasis, lymphoid nodal status, sex, or age. Patients with late-stage gastric adenocarcinoma had a higher amount of tumor cells showing positive staining for C5b-9 than patients with early-stage disease. These results may help in diagnosis and assessment of disease severity of human gastric carcinoma.

  13. Mesenchymal Stem Cells Control Complement C5 Activation by Factor H in Lupus Nephritis

    Directory of Open Access Journals (Sweden)

    Haijun Ma

    2018-06-01

    Full Text Available Lupus nephritis (LN is one of the most severe complications of systemic lupus erythematosus (SLE caused by uncontrolled activation of the complement system. Mesenchymal stem cells (MSCs exhibit clinical efficacy for severe LN in our previous studies, but the underlying mechanisms of MSCs regulating complement activation remain largely unknown. Here we show that significantly elevated C5a and C5b-9 were found in patients with LN, which were notably correlated with proteinuria and different renal pathological indexes of LN. MSCs suppressed systemic and intrarenal activation of C5, increased the plasma levels of factor H (FH, and ameliorated renal disease in lupus mice. Importantly, MSCs transplantation up-regulated the decreased FH in patients with LN. Mechanistically, interferon-α enhanced the secretion of FH by MSCs. These data demonstrate that MSCs inhibit the activation of pathogenic C5 via up-regulation of FH, which improves our understanding of the immunomodulatory mechanisms of MSCs in the treatment of lupus nephritis. Keywords: Lupus nephritis, C5, MSCs, FH

  14. A Novel Role for C5a in B-1 Cell Homeostasis

    Directory of Open Access Journals (Sweden)

    Katharina Bröker

    2018-02-01

    Full Text Available B-1 cells constitute a unique subpopulation of lymphocytes residing mainly in body cavities like the peritoneal cavity (PerC but are also found in spleen and bone marrow (BM. As innate-like B cells, they mediate first line immune defense through low-affinity natural IgM (nIgM antibodies. PerC B-1 cells can egress to the spleen and differentiate into nIgM antibody-secreting plasma cells that recognize conserved exogenous and endogenous cellular structures. Homing to and homeostasis within the PerC are regulated by the chemokine CXCL13 released by PerC macrophages and stroma cells. However, the exact mechanisms underlying the regulation of CXCL13 and B-1 homeostasis are not fully explored. B-1 cells play important roles in the inflammatory response to infection, autoimmunity, ischemia/reperfusion injury, obesity, and atherosclerosis. Remarkably, this list of inflammatory entities has a strong overlap with diseases that are regulated by complement suggesting a link between B-1 cells and the complement system. Interestingly, up to now, no data exist regarding the role of complement in B-1 cell biology. Here, we demonstrate for the first time that C5a regulates B-1 cell steady-state dynamics within the peritoneum, the spleen, and the BM. We found decreased B-1a cell numbers in the peritoneum and the spleen of C5aR1−/− mice associated with increased B1-a and B1-b numbers in the spleen and high serum titers of nIgM antibodies directed against phosphorylcholine and several pneumococcal polysaccharides. Similarly, peritoneal B-1a cells were decreased in the peritoneum and splenic B-1a and B-1b cells were increased in C5aR2−/− mice. The decrease in peritoneal B-1 cell numbers was associated with decreased peritoneal CXCL13 levels in C5aR1−/− and C5aR2−/− mice. In search for mechanisms, we found that combined TLR2 and IL-10 receptor activation in PerC macrophages induced strong CXCL13 production, which was significantly reduced in cells

  15. Deposition Velocities of C1 - C5 Alkyl Nitrates at a Northern Colorado Site

    Science.gov (United States)

    Abeleira, A.; Sive, B. C.; Farmer, D.; Swarthout, B.

    2017-12-01

    Organic nitrates (RONO2) are ubiquitous in the troposphere and are part of gas-phase oxidized nitrogen (NOy = NOx + HNO3 + HONO + N2O5 + HO2NO2 + PAN + NO3 + RONO2). RONO2 can act as both sinks and sources of HOx (RO + RO2 + OH) and NOx (NO + NO2), contributing to the nonlinearity of ozone (O3) formation. It is thus potentially important to understand sinks of RONO2, and how they change seasonally, in order to predict O3 on local, regional and global scales. We focus here on speciated C1 - C5 monofunctional alkyl nitrates (C1 - C5 ANs). In polluted continental regions the dominant source of C1 - C5 ANs is the OH-initiated oxidation of parent alkanes in the presence of NO, and thus changes seasonally with OH mixing ratios. Direct emissions of C1 - C2 ANs include oceanic sources and biomass burning. The sinks of C1 - C5 ANs include OH oxidation and photolysis, both of which release O3 precursors. Chemical transport models tend to overestimate the mixing ratios of small ANs indicating that a missing sink is not included. Wet deposition of C1 - C5 ANs is typically ignored due to the very low Henry's Law constants of these species. However, dry deposition of total organic nitrogen has been observed to be substantial. The dry deposition velocity of methyl nitrate has previously been estimated from summer observations at a rural New England site with a value of 0.13 cm s-1. Here we report deposition velocities for C1 - C5 ANs from surface observations at the Boulder Atmospheric Observatory (BAO) in Erie, Colorado during winter 2011 and spring 2015. We calculate deposition velocities from the observed decay in C1 - C5 ANs at night during periods with a stable nocturnal boundary layer height of 100 - 200 meters. Ideal meteorological conditions were observed for 5 nights during the 2011 NACHTT campaign (February - March 2011), and for 5 nights during the 2015 SONGNEX campaign (March - May 2015). Deposition velocities increased with alkyl nitrate size, ranging from 0.15 cm

  16. Deletion of the complement C5a receptor alleviates the severity of acute pneumococcal otitis media following influenza A virus infection in mice.

    Directory of Open Access Journals (Sweden)

    Hua Hua Tong

    Full Text Available There is considerable evidence that influenza A virus (IAV promotes adherence, colonization, and superinfection by S. pneumoniae (Spn and contributes to the pathogenesis of otitis media (OM. The complement system is a critical innate immune defense against both pathogens. To assess the role of the complement system in the host defense and the pathogenesis of acute pneumococcal OM following IAV infection, we employed a well-established transtympanically-induced mouse model of acute pneumococcal OM. We found that antecedent IAV infection enhanced the severity of acute pneumococcal OM. Mice deficient in complement C1qa (C1qa-/- or factor B (Bf -/- exhibited delayed viral and bacterial clearance from the middle ear and developed significant mucosal damage in the eustachian tube and middle ear. This indicates that both the classical and alternative complement pathways are critical for the oto-immune defense against acute pneumococcal OM following influenza infection. We also found that Spn increased complement activation following IAV infection. This was characterized by sustained increased levels of anaphylatoxins C3a and C5a in serum and middle ear lavage samples. In contrast, mice deficient in the complement C5a receptor (C5aR demonstrated enhanced bacterial clearance and reduced severity of OM. Our data support the concept that C5a-C5aR interactions play a significant role in the pathogenesis of acute pneumococcal OM following IAV infection. It is possible that targeting the C5a-C5aR axis might prove useful in attenuating acute pneumococcal OM in patients with influenza infection.

  17. Inhibition of HIV-1 in vitro by C-5 propyne phosphorothioate antisense to rev

    DEFF Research Database (Denmark)

    Lund, O S; Nielsen, Jens Ole; Hansen, J E

    1995-01-01

    in a sequence-dependent manner. The antiviral effect was obtained by lipofection or simple addition of 0.2-1 microM modified oligodeoxynucleotide to the culture medium of H9 cells chronically infected with the HIV-1LAI isolate of human immunodeficiency virus type 1. We conclude that C-5 propyne...

  18. C2-C5 hydrocarbon measurements in the Netherlands 1981-1991

    NARCIS (Netherlands)

    Roemer, M.; Builtjes, P.; Esser, P.; Guicherit, R.; Thijsse, T.

    1999-01-01

    Measurements of C2-C5 hydrocarbons on an hourly basis at the TNO site in Delft from 1982 to 1984 and at Moerdijk over the period 1981-1991 are presented. In combination with meteorological data (wind direction and wind speed) the Delft and Moerdijk series are evaluated to identify source categories,

  19. Bill C-5, an act to amend the radiation emitting devices act

    International Nuclear Information System (INIS)

    1984-01-01

    This Act, entitled Bill C-5, allows for a series of amendments to the Radiation Emitting Devices Act. The amendments relate to regulations concerned with the sale, lease or import, labelling, advertising, packaging, safety standards and inspection of radiation emitting devices

  20. Interactive analysis of human error factors in NPP operation events

    International Nuclear Information System (INIS)

    Zhang Li; Zou Yanhua; Huang Weigang

    2010-01-01

    Interactive of human error factors in NPP operation events were introduced, and 645 WANO operation event reports from 1999 to 2008 were analyzed, among which 432 were found relative to human errors. After classifying these errors with the Root Causes or Causal Factors, and then applying SPSS for correlation analysis,we concluded: (1) Personnel work practices are restricted by many factors. Forming a good personnel work practices is a systematic work which need supports in many aspects. (2)Verbal communications,personnel work practices, man-machine interface and written procedures and documents play great roles. They are four interaction factors which often come in bundle. If some improvements need to be made on one of them,synchronous measures are also necessary for the others.(3) Management direction and decision process, which are related to management,have a significant interaction with personnel factors. (authors)

  1. 17 CFR 240.19c-5 - Governing the multiple listing of options on national securities exchanges.

    Science.gov (United States)

    2010-04-01

    ... of options on national securities exchanges. 240.19c-5 Section 240.19c-5 Commodity and Securities... of Exchange Members § 240.19c-5 Governing the multiple listing of options on national securities exchanges. (a) The rules of each national securities exchange that provides a trading market in standardized...

  2. Effective ionization coefficient of C5 perfluorinated ketone and its mixtures with air

    Science.gov (United States)

    Aints, Märt; Jõgi, Indrek; Laan, Matti; Paris, Peeter; Raud, Jüri

    2018-04-01

    C5 perfluorinated ketone (C5 PFK with UIPAC chemical name 1,1,1,3,4,4,4-heptafluoro-3-(trifluoromethyl)-2-butanone and sold by 3M as Novec™ 5110) has a high dielectric strength and a low global warming potential, which makes it interesting as an insulating gas in medium and high-voltage applications. The study was carried out to determine the effective Townsend ionization coefficient α eff as a function of electric field strength and gas density for C5 PFK and for its mixtures with air. The non-self-sustained Townsend discharge between parallel plate electrodes was initiated by illuminating the cathode by UV radiation. The discharge current, I, was measured as a function of inter-electrode distance, d, at different gas densities, N, and electric field strengths, E. The effective ionization coefficient α eff was determined from the semi-logarithmic plots of I/I 0 against d. For each tested gas mixture, the density normalized effective ionization coefficient α eff/N was found to be a unique function of reduced electric field strength E/N. The measurements were carried out in the absolute pressure range of 0.05-1.3 bar and E/N range of 150-1200 Td. The increasing fraction of C5 PFK in air resulted in the decrease of effective ionization coefficient. The limiting electric field strength (E/N)lim where the effective ionization coefficient α eff became zero was 770 Td (190 kV cm-1 at 1 bar) for pure C5 PFK and decreased to 225 Td (78 kV cm-1 at 1.4 bar) for 7.6% C5 PFK/air mixture. The latter value of (E/N)lim is still more than two times higher than the (E/N)lim value of synthetic air and about two-thirds of the value corresponding to pure SF6. The investigated gas mixtures have the potential to become an alternative to SF6 in numerous high- and medium-voltage applications.

  3. Granulocyte and monocyte CD11b expression during plasma separation is dependent on complement factor 5 (C5) - an ex vivo study with blood from a C5-deficient individual.

    Science.gov (United States)

    Hardersen, Randolf; Enebakk, Terje; Christiansen, Dorte; Bergseth, Grethe; Brekke, Ole-Lars; Mollnes, Tom Eirik; Lappegård, Knut Tore; Hovland, Anders

    2018-04-01

    The aim of the study was to investigate the role of complement factor 5 (C5) in reactions elicited by plasma separation using blood from a C5-deficient (C5D) individual, comparing it to C5-deficient blood reconstituted with C5 (C5DR) and blood from healthy donors. Blood was circulated through an ex vivo plasma separation model. Leukocyte CD11b expression and leukocyte-platelet conjugates were measured by flow cytometry during a 30-min period. Other markers were assessed during a 240-min period. Granulocyte and monocyte CD11b expression did not increase in C5D blood during plasma separation. In C5DR samples granulocytes CD11b expression, measured by mean fluorescence intensity (MFI), increased from 10481 ± 6022 (SD) to 62703 ± 4936, and monocytes CD11b expression changed from 13837 ± 7047 to 40063 ± 713. Granulocyte-platelet conjugates showed a 2.5-fold increase in the C5DR sample compared to the C5D sample. Monocyte-platelet conjugates increased independently of C5. In the C5D samples, platelet count decreased from 210 × 10 9 /L (201-219) (median and range) to 51 × 10 9 /L (50-51), and C3bc increased from 14 CAU/mL (21-7) to 198 CAU/mL (127-269), whereas TCC formation was blocked during plasma separation. In conclusion, up-regulation of granulocyte and monocyte CD11b during plasma separation was C5-dependent. The results also indicate C5 dependency in granulocyte-platelet conjugates formation. © 2018 APMIS. Published by John Wiley & Sons Ltd.

  4. Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery

    Directory of Open Access Journals (Sweden)

    Reza Rezvani

    2014-01-01

    Full Text Available Objective. Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation. We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2 would be associated with pre- or postmenopausal status and metabolic profile in severely obese women. We hypothesized that C5L2/C5aR ratio, potentially influencing the ASP/C5L2 metabolic versus C5a/C5aR immune response, would predict metabolic profiles after weight loss surgery. Materials and Methods. Fasting plasma (hormone, lipid, and enzyme analysis and liver biopsies (RT-PCR gene expression were obtained from 91 women during surgery. Results. Hepatic C5L2 mRNA expression was elevated in pre- versus postmenopausal women (P<0.01 and correlated positively with circulating estradiol, estrone, ApoB, ApoA1, ApoA1/B, waist circumference, age, and LDL-C (all P<0.05. While plasma ASP was lower in pre- versus postmenopausal women (P<0.01, the hepatic C5L2/C5aR mRNA ratio was increased (P<0.001 and correlated positively with estrone (P<0.01 and estradiol (P<0.001 and negatively with circulating ApoB and liver enzymes ALT, AST, and GGT (all P<0.05. Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA. Conclusion. C5L2-C5aR association with other mediators including estrogens may contribute to hepatic metabolic and inflammatory function.

  5. Optical waveguides in LiTaO3 crystals fabricated by swift C5+ ion irradiation

    International Nuclear Information System (INIS)

    Liu, Guiyuan; He, Ruiyun; Akhmadaliev, Shavkat; Vázquez de Aldana, Javier R.; Zhou, Shengqiang; Chen, Feng

    2014-01-01

    We report on the optical waveguides, in both planar and ridge configurations, fabricated in LiTaO 3 crystal by using carbon (C 5+ ) ions irradiation at energy of 15 MeV. The planar waveguide was produced by direct irradiation of swift C 5+ ions, whilst the ridge waveguides were manufactured by using femtosecond laser ablation of the planar layer. The reconstructed refractive index profile of the planar waveguide has showed a barrier-shaped distribution, and the near-field waveguide mode intensity distribution was in good agreement with the calculated modal profile. After thermal annealing at 260 °C in air, the propagation losses of both the planar and ridge waveguides were reduced to 10 dB/cm

  6. Release of (14C)5-hydroxytryptamine from human platelets by red wine

    International Nuclear Information System (INIS)

    Jarman, J.; Glover, V.; Sandler, M.

    1991-01-01

    Red wine, at a final dilution of 1/50, caused released of ( 14 C)5-hydroxytryptamine (5-HT) from preloaded platelets, an effect which was not observed with any white wines or beers tested. Since 5-HT, is probably released from body stores during migraine attacks and red wine is known to provoke migraine episodes in susceptible individuals, release of 5-HT, possibly from central stores, could represent a plausible mechanism for its mode of action

  7. Synthesis and thermolysis of Cp*(C5Me4CH2)TiR complexes

    NARCIS (Netherlands)

    Luinstra, GA; Brinkmann, PHP; Teuben, JH; Luinstra, Gerrit A.

    1997-01-01

    Substitution of the chloride in Cp*FvTiCl with MR (Fv = C5Me4CH2; R = Me, CH2SiMe3, CH2CMe3, CH = CH2, M = Li; R = CH2Ph, M = K; R = C3H5, M = MgCl; R = Ph, M = Na . NaCl) gives Cp*FvTiR. NMR spectroscopic evidence points towards a series of structurally related compounds with a bent-sandwich

  8. Comparison of neutral density profiles measured using Dα and C5+ in NSTX-U

    Science.gov (United States)

    Bell, R. E.; Scotti, F.; Diallo, A.; Leblanc, B. P.; Podesta, M.; Sabbagh, S. A.

    2017-10-01

    Edge neutral density profiles determined from two different measurements are compared on NSTX-U plasmas. Neutral density measurements were not typical on NSTX plasmas. An array of fibers dedicated to the measurement of passive emission of C5+, used to subtract background emission for charge exchange recombination spectroscopy (CHERS), can be used to infer deuterium neutral density near the plasma edge. The line emission from C5+ is dominated by charge exchange with neutral deuterium near the plasma edge. An edge neutral density diagnostic consisting of a camera with a Dα filter was installed on NSTX-U. The line-integrated measurements from both diagnostics are inverted to obtain local emissivity profiles. Neutral density is then inferred using atomics rates from ADAS and profile measurements from Thomson scattering and CHERS. Comparing neutral density profiles from the two diagnostic measurements helps determine the utility of using the more routinely available C5+ measurements for neutral density profiles. Initial comparisons show good agreement between the two measurements inside the separatrix. Supported by US DoE Contracts DE-AC02-09CH11466 and DE-AC52-07NA27344.

  9. TrpC5 Mediates Acute Leptin and Serotonin Effects via Pomc Neurons

    Directory of Open Access Journals (Sweden)

    Yong Gao

    2017-01-01

    Full Text Available The molecular mechanisms underlying acute leptin and serotonin 2C receptor-induced hypophagia remain unclear. Here, we show that neuronal and pro-opiomelanocortin (Pomc-specific loss of transient receptor potential cation 5 (TrpC5 subunits is sufficient to decrease energy expenditure and increase food intake resulting in elevated body weight. Deficiency of Trpc5 subunits in Pomc neurons is also sufficient to block the anorexigenic effects of leptin and serotonin 2C receptor (Ht2Cr agonists. The loss of acute anorexigenic effects of these receptors is concomitant with a blunted electrophysiological response to both leptin and Ht2Cr agonists in arcuate Pomc neurons. We also demonstrate that the Ht2Cr agonist lorcaserin-induced improvements in glucose and insulin tolerance are blocked by TrpC5 deficiency in Pomc neurons. Together, our results link TrpC5 subunits in the brain with leptin- and serotonin 2C receptor-dependent changes in neuronal activity, as well as energy balance, feeding behavior, and glucose metabolism.

  10. Molecular and expression analysis of complement component C5 in the nurse shark (Ginglymostoma cirratum) and its predicted functional role.

    Science.gov (United States)

    Graham, Matthew; Shin, Dong-Ho; Smith, Sylvia L

    2009-07-01

    We present the complete cDNA sequence of shark (Ginglymostoma cirratum) pro-C5 and its molecular characterization with a descriptive analysis of the structural elements necessary for its potential functional role as a potent mediator of inflammation (fragment C5a) and initiator molecule (fragment C5b) for the assembly of the membrane attack complex (MAC) upon activation by C5 convertase. In mammals the three complement activation cascades, the classical, alternative and lectin pathways, converge at the activation of C3, a pivotal complement protein. It is, however, the subsequent activation of the next complement component, C5, which is the focal point at which the initiation of the terminal lytic pathway takes place and involves the stepwise assembly of the MAC. The effector cytolytic function of complement occurs with the insertion of MAC into target membranes causing dough-nut like holes and cell leakage. The lytic activity of shark complement results in structurally similar holes in target membranes suggesting the assembly of a shark MAC that likely involves a functional analogue of C5. The composition of shark MAC remains unresolved and to date conclusive evidence has been lacking for shark C5. The gene has not been cloned nor has the serum protein been characterized for any elasmobranch species. This report is the first to confirm the presence of C5 homologue in the shark. GcC5 is remarkably similar to human C5 in overall structure and domain arrangement. The GcC5 cDNA measured 5160-bp with 5' and 3' UTRs of 35 bp and 79 bp, respectively. Structural analysis of the derived protein sequence predicts a molecule that is a two-chain structure which lacks a thiolester bond and contains a C5 convertase cleavage site indicating that activation will generate two peptides, akin to C5b and C5a. The putative GcC5 molecule also contains the C-terminal C345C/Netrin module that characterizes C3, C4 and C5. Multiple alignment of deduced amino acid sequences shows that GcC5

  11. Cloning, expression, cellular distribution, and role in chemotaxis of a C5a receptor in rainbow trout: the first identification of a C5a receptor in a nonmammalian species

    Science.gov (United States)

    Boshra, Hani; Li, Jun; Peters, Rodney; Hansen, John; Matlapudi, Anjan; Sunyer, J. Oriol

    2004-01-01

    C3a, C4a, and C5a anaphylatoxins generated during complement activation play a key role in inflammation. C5a is the most potent of the three anaphylatoxins in eliciting biological responses. The effects of C5a are mediated by its binding to C5a receptor (C5aR, CD88). To date, C5aR has only been identified and cloned in mammalian species, and its evolutionary history remains ill-defined. To gain insights into the evolution, conserved structural domains, and functions of C5aR, we have cloned and characterized a C5aR in rainbow trout, a teleost fish. The isolated cDNA encoded a 350-aa protein that showed the highest sequence similarity to C5aR from other species. Genomic analysis revealed the presence of one continuous exon encoding the entire open reading frame. Northern blot analysis showed significant expression of the trout C5a receptor (TC5aR) message in PBLs and kidney. Flow cytometric analysis showed that two Abs generated against two different areas of the extracellular N-terminal region of TC5aR positively stained the same leukocyte populations from PBLs. B lymphocytes and granulocytes comprised the majority of cells recognized by the anti-TC5aR. More importantly, these Abs inhibited chemotaxis of PBLs toward a chemoattractant fraction purified from complement-activated trout serum. Our data suggest that the split between C5aR and C3aR from a common ancestral molecule occurred before the emergence of teleost fish. Moreover, we demonstrate that the overall structure of C5aR as well as its role in chemotaxis have remained conserved for >300 million years.

  12. Analysis on Biomechanical Characteristics of Post-operational Vertebral C5-C6 Segments

    Directory of Open Access Journals (Sweden)

    Heqiang Tian

    2016-03-01

    Full Text Available Both anterior cervical decompression and fusion (ACDF and artificial cervical disc replacement (ACDR have obvious advantages in the treatment of cervical spondylosis. To analyze the operation results, it is absolutely necessary to study the biomechanics of the movement range of post-operational vertebral C5-C6 segments, especially the biomechanical characteristics in cervical tissues in actual movements. In this study, using the human vertebral 3D graph gained by imaging diagnosis (CT, a vertebral solid model is established by the 3D reconstruction algorithm and reverse engineering technology. After that, with cervical soft tissue structure added to the solid model and set with a joint contact mechanism, a finite element model with a complete, accurate cervical C5-C6 kinematic unit is constructed, based on relevant physiological anatomical knowledge. This model includes vertebral segments, an intervertebral disc, ligament and zygopophysis in the cervical C5-C6 kinematic unit. In the created vertebral finite element model, the model is amended, referring to ACDF and ACDR, and the load and constraint are applied to a normal group, a fusion group and a displacement group, so as to analyze the biomechanical characteristics of the cervical vertebra after ACDF and ACDR. By comparing the finite element simulation results of different surgeries, this paper is intended to evaluate the functions and biomechanical behaviors of the post-operational vertebra, and explore the influence of the operation on the biomechanical stability of the cervical vertebra. This will provide theoretical guidance for implementation and optimization of ACDF and ACDR.

  13. Heparan sulfate C5-epimerase is essential for heparin biosynthesis in mast cells.

    Science.gov (United States)

    Feyerabend, Thorsten B; Li, Jin-Ping; Lindahl, Ulf; Rodewald, Hans-Reimer

    2006-04-01

    Biosynthesis of heparin, a mast cell-derived glycosaminoglycan with widespread importance in medicine, has not been fully elucidated. In biosynthesis of heparan sulfate (HS), a structurally related polysaccharide, HS glucuronyl C5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) residues. We have generated Hsepi-null mouse mutant mast cells, and we show that the same enzyme catalyzes the generation of IdoA in heparin and that 'heparin' lacking IdoA shows a distorted O-sulfation pattern.

  14. C5a Receptor (CD88) Blockade Protects against MPO-ANCA GN

    OpenAIRE

    Xiao, Hong; Dairaghi, Daniel J.; Powers, Jay P.; Ertl, Linda S.; Baumgart, Trageen; Wang, Yu; Seitz, Lisa C.; Penfold, Mark E.T.; Gan, Lin; Hu, Peiqi; Lu, Bao; Gerard, Norma P.; Gerard, Craig; Schall, Thomas J.; Jaen, Juan C.

    2013-01-01

    Necrotizing and crescentic GN (NCGN) with a paucity of glomerular immunoglobulin deposits is associated with ANCA. The most common ANCA target antigens are myeloperoxidase (MPO) and proteinase 3. In a manner that requires activation of the alternative complement pathway, passive transfer of antibodies to mouse MPO (anti-MPO) induces a mouse model of ANCA NCGN that closely mimics human disease. Here, we confirm the importance of C5aR/CD88 in the mediation of anti-MPO–induced NCGN and report th...

  15. Intelligent Diagnostic Assistant for Complicated Skin Diseases through C5's Algorithm.

    Science.gov (United States)

    Jeddi, Fatemeh Rangraz; Arabfard, Masoud; Kermany, Zahra Arab

    2017-09-01

    Intelligent Diagnostic Assistant can be used for complicated diagnosis of skin diseases, which are among the most common causes of disability. The aim of this study was to design and implement a computerized intelligent diagnostic assistant for complicated skin diseases through C5's Algorithm. An applied-developmental study was done in 2015. Knowledge base was developed based on interviews with dermatologists through questionnaires and checklists. Knowledge representation was obtained from the train data in the database using Excel Microsoft Office. Clementine Software and C5's Algorithms were applied to draw the decision tree. Analysis of test accuracy was performed based on rules extracted using inference chains. The rules extracted from the decision tree were entered into the CLIPS programming environment and the intelligent diagnostic assistant was designed then. The rules were defined using forward chaining inference technique and were entered into Clips programming environment as RULE. The accuracy and error rates obtained in the training phase from the decision tree were 99.56% and 0.44%, respectively. The accuracy of the decision tree was 98% and the error was 2% in the test phase. Intelligent diagnostic assistant can be used as a reliable system with high accuracy, sensitivity, specificity, and agreement.

  16. 3-D extension C5G7 MOX benchmark calculation using threedant code

    International Nuclear Information System (INIS)

    Kim, H.Ch.; Han, Ch.Y.; Kim, J.K.; Na, B.Ch.

    2005-01-01

    It pursued the benchmark on deterministic 3-D MOX fuel assembly transport calculations without spatial homogenization (C5G7 MOX Benchmark Extension). The goal of this benchmark is to provide a more through test results for the abilities of current available 3-D methods to handle the spatial heterogeneities of reactor core. The benchmark requires solutions in the form of normalized pin powers as well as the eigenvalue for each of the control rod configurations; without rod, with A rods, and with B rods. In this work, the DANTSYS code package was applied to analyze the 3-D Extension C5G7 MOX Benchmark problems. The THREEDANT code within the DANTSYS code package, which solves the 3-D transport equation in x-y-z, and r-z-theta geometries, was employed to perform the benchmark calculations. To analyze the benchmark with the THREEDANT code, proper spatial and angular approximations were made. Several calculations were performed to investigate the effects of the different spatial approximations on the accuracy. The results from these sensitivity studies were analyzed and discussed. From the results, it is found that the 4*4 grid per pin cell is sufficiently refined so that very little benefit is obtained by increasing the mesh size. (authors)

  17. C5 Benchmark Problem with Discrete Ordinate Radiation Transport Code DENOVO

    Energy Technology Data Exchange (ETDEWEB)

    Yesilyurt, Gokhan [ORNL; Clarno, Kevin T [ORNL; Evans, Thomas M [ORNL; Davidson, Gregory G [ORNL; Fox, Patricia B [ORNL

    2011-01-01

    The C5 benchmark problem proposed by the Organisation for Economic Co-operation and Development/Nuclear Energy Agency was modeled to examine the capabilities of Denovo, a three-dimensional (3-D) parallel discrete ordinates (S{sub N}) radiation transport code, for problems with no spatial homogenization. Denovo uses state-of-the-art numerical methods to obtain accurate solutions to the Boltzmann transport equation. Problems were run in parallel on Jaguar, a high-performance supercomputer located at Oak Ridge National Laboratory. Both the two-dimensional (2-D) and 3-D configurations were analyzed, and the results were compared with the reference MCNP Monte Carlo calculations. For an additional comparison, SCALE/KENO-V.a Monte Carlo solutions were also included. In addition, a sensitivity analysis was performed for the optimal angular quadrature and mesh resolution for both the 2-D and 3-D infinite lattices of UO{sub 2} fuel pin cells. Denovo was verified with the C5 problem. The effective multiplication factors, pin powers, and assembly powers were found to be in good agreement with the reference MCNP and SCALE/KENO-V.a Monte Carlo calculations.

  18. CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a

    Directory of Open Access Journals (Sweden)

    Rachel Vaivoda

    2015-01-01

    Full Text Available CYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4. CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation. We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in vitro assay. There was no significant difference in the chemotactic response to LTB4, but the response to complement component C5a increased 1.9–2.25-fold in knockout cells compared to wild-type (P < 0.01. This increase was still observed when neutrophils were treated with inhibitors of eicosanoid synthesis. There were no changes in expression of other CYP4 enzymes in knockout neutrophils that might compensate for loss of CYP4F18 or lead to differences in activity. A mouse model of dextran sodium sulfate colitis was used to investigate the consequences of increased C5a-dependent chemotaxis in vivo, but there was no significant difference in weight loss, disease activity, or colonic tissue myeloperoxidase between wild-type and Cyp4f18 knockout mice. This study demonstrates the limitations of inferring CYP4F function based on an ability to use LTB4 as a substrate, points to expanding roles for CYP4F enzymes in immune regulation, and underscores the in vivo challenges of CYP knockout studies.

  19. Crystallographic characterization of divalent organosamarium compound (C5H5)2Sm(THF)2

    International Nuclear Information System (INIS)

    Jagannatha Swamy, S.

    2002-01-01

    The single pot reaction between SmX 2 (X = Cl - , I - ) and BuLi in THF at -40 degC, followed by the addition of C 5 H 5 - Na + results in a dark red solution. Leaving the concentrated reaction mixture at -25 degC for two days in a deep freezer results in the formation of the crystals of the compound, (C 5 H 5 ) 2 ; Sm(THF) 2 . The compound is insoluble in any solvent and it has been characterized by conventional methods. The crystals are monoclinic with space group C2/c, and a = 13.416(1), b = 9.644(1), c = 14.129(2) pm, β109.873(9) 0 and z = 4 for ρcalcd = 1.64 g cm -3 . Least squares refinement on the basis of 1804 observed reflections has led to a final R value of 0.037 and R w = 0.054. (author)

  20. Interacting with accelerators

    International Nuclear Information System (INIS)

    Dasgupta, S.

    1994-01-01

    Accelerators are research machines which produce energetic particle beam for use as projectiles to effect nuclear reactions. These machines along with their services and facilities may occupy very large areas. The man-machine interface of accelerators has evolved with technological changes in the computer industry and may be partitioned into three phases. The present paper traces the evolution of man-machine interface from the earliest accelerators to the present computerized systems incorporated in modern accelerators. It also discusses the advantages of incorporating expert system technology for assisting operators. (author). 8 ref

  1. Structural analysis of CYP2C9 and CYP2C5 and an evaluation of commonly used molecular modeling techniques

    DEFF Research Database (Denmark)

    Afzelius, Lovisa; Raubacher, Florian; Karlén, Anders

    2004-01-01

    , newly built homology models, and repeated molecular dynamics simulations. The CPCA was based on molecular interaction fields focused on the active site regions of the proteins and include detailed amino acid analysis. The comparison of the CYP2C9 and CYP2C5 crystal structures revealed differences...... improved the similarity to the crystal target in some cases and could be recommended even though it requires a careful manual alignment process. The application of molecular dynamics simulations to highly flexible proteins such as cytochromes P450 is also explored and the information is extracted...... in the flexible regions such as the B-C and F-G loop and the N and C termini. Cross homology models of CYP2C9 and CYP2C5, using their respective crystal structures as templates, indicated that such models were more similar to their templates than to their target proteins. Inclusion of multiple templates slightly...

  2. Scientific Impact of MODIS C5 Calibration Degradation and C6+ Improvements

    Science.gov (United States)

    Lyapustin, A.; Wang, Y.; Xiong, X.; Meister, G.; Platnick, S.; Levy, R.; Franz, B.; Korkin, S.; Hilker, T.; Tucker, J.; hide

    2014-01-01

    The Collection 6 (C6) MODIS (Moderate Resolution Imaging Spectroradiometer) land and atmosphere data sets are scheduled for release in 2014. C6 contains significant revisions of the calibration approach to account for sensor aging. This analysis documents the presence of systematic temporal trends in the visible and near-infrared (500 m) bands of the Collection 5 (C5) MODIS Terra and, to lesser extent, in MODIS Aqua geophysical data sets. Sensor degradation is largest in the blue band (B3) of the MODIS sensor on Terra and decreases with wavelength. Calibration degradation causes negative global trends in multiple MODIS C5 products including the dark target algorithm's aerosol optical depth over land and Ångstrom exponent over the ocean, global liquid water and ice cloud optical thickness, as well as surface reflectance and vegetation indices, including the normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI). As the C5 production will be maintained for another year in parallel with C6, one objective of this paper is to raise awareness of the calibration-related trends for the broad MODIS user community. The new C6 calibration approach removes major calibrations trends in the Level 1B (L1B) data. This paper also introduces an enhanced C6C calibration of the MODIS data set which includes an additional polarization correction (PC) to compensate for the increased polarization sensitivity of MODIS Terra since about 2007, as well as detrending and Terra- Aqua cross-calibration over quasi-stable desert calibration sites. The PC algorithm, developed by the MODIS ocean biology processing group (OBPG), removes residual scan angle, mirror side and seasonal biases from aerosol and surface reflectance (SR) records along with spectral distortions of SR. Using the multiangle implementation of atmospheric correction (MAIAC) algorithm over deserts, we have also developed a detrending and cross-calibration method which removes residual decadal trends on

  3. Relationship between a novel polymorphism of the C5L2 gene and coronary artery disease.

    Directory of Open Access Journals (Sweden)

    Ying-Ying Zheng

    Full Text Available BACKGROUND: C5L2 has been demonstrated to be a functional receptor of acylation-stimulating protein (ASP, which is a stimulator of triglyceride synthesis or glucose transport. However, little is known about the variations in the coding region of the C5L2 gene and their association with coronary artery disease (CAD. METHODOLOGY/PRINCIPAL FINDINGS: We identified a novel single nucleotide polymorphism (SNP, 698C>T (P233L, in exon 2 using a polymerase chain reaction direct-sequencing method. This nucleotide change causes the amino-acid order from proline to leucine at codon 233. We examined the role of this SNP for CAD using two independent case-control studies: one was in the Han population (492 CAD patients and 577 control subjects and the other was in the Uygur population (319 CAD patients and 554 control subjects. Heterozygote carriers of the 698CT genotype were more frequent among CAD patients than among controls not only in the Han population (7.3% versus 1.7% but also in the Uygur population (4.7% versus 1.6%. The odds ratio (OR for carriers of the 698CT genotype for CAD was 4.484 (95% confidence interval (CI: 2.197-9.174 in the Han group and 2.989 (95% CI: 1.292-6.909 in the Uygur population. After adjustment of confounding factors such as sex, age, smoking, alcohol consumption, hypertension, diabetes, as well as serum levels of triglyceride, total cholesterol, high-density lipoprotein, the difference remained significant in the Han group (P<0.001, OR = 6.604, 95% CI: 2.776-15.711 and in the Uygur group (P = 0.047, OR = 2.602, 95% CI: 1.015-6.671. CONCLUSION/SIGNIFICANCE: The 698CT genotype of C5L2 may be a genetic maker of CAD in the Han and Uygur population in western China.

  4. INTERACT

    DEFF Research Database (Denmark)

    Jochum, Elizabeth; Borggreen, Gunhild; Murphey, TD

    This paper considers the impact of visual art and performance on robotics and human-computer interaction and outlines a research project that combines puppetry and live performance with robotics. Kinesics—communication through movement—is the foundation of many theatre and performance traditions ...

  5. Verification of the shift Monte Carlo code with the C5G7 reactor benchmark

    International Nuclear Information System (INIS)

    Sly, N. C.; Mervin, B. T.; Mosher, S. W.; Evans, T. M.; Wagner, J. C.; Maldonado, G. I.

    2012-01-01

    Shift is a new hybrid Monte Carlo/deterministic radiation transport code being developed at Oak Ridge National Laboratory. At its current stage of development, Shift includes a parallel Monte Carlo capability for simulating eigenvalue and fixed-source multigroup transport problems. This paper focuses on recent efforts to verify Shift's Monte Carlo component using the two-dimensional and three-dimensional C5G7 NEA benchmark problems. Comparisons were made between the benchmark eigenvalues and those output by the Shift code. In addition, mesh-based scalar flux tally results generated by Shift were compared to those obtained using MCNP5 on an identical model and tally grid. The Shift-generated eigenvalues were within three standard deviations of the benchmark and MCNP5-1.60 values in all cases. The flux tallies generated by Shift were found to be in very good agreement with those from MCNP. (authors)

  6. Attila calculations for the 3-D C5G7 benchmark extension

    International Nuclear Information System (INIS)

    Wareing, T.A.; McGhee, J.M.; Barnett, D.A.; Failla, G.A.

    2005-01-01

    The performance of the Attila radiation transport software was evaluated for the 3-D C5G7 MOX benchmark extension, a follow-on study to the MOX benchmark developed by the 'OECD/NEA Expert Group on 3-D Radiation Transport Benchmarks'. These benchmarks were designed to test the ability of modern deterministic transport methods to model reactor problems without spatial homogenization. Attila is a general purpose radiation transport software package with an integrated graphical user interface (GUI) for analysis, set-up and postprocessing. Attila provides solutions to the discrete-ordinates form of the linear Boltzmann transport equation on a fully unstructured, tetrahedral mesh using linear discontinuous finite-element spatial differencing in conjunction with diffusion synthetic acceleration of inner iterations. The results obtained indicate that Attila can accurately solve the benchmark problem without spatial homogenization. (authors)

  7. Pericles and Attila results for the C5G7 MOX benchmark problems

    International Nuclear Information System (INIS)

    Wareing, T.A.; McGhee, J.M.

    2002-01-01

    Recently the Nuclear Energy Agency has published a new benchmark entitled, 'C5G7 MOX Benchmark.' This benchmark is to test the ability of current transport codes to treat reactor core problems without spatial homogenization. The benchmark includes both a two- and three-dimensional problem. We have calculated results for these benchmark problems with our Pericles and Attila codes. Pericles is a one-,two-, and three-dimensional unstructured grid discrete-ordinates code and was used for the twodimensional benchmark problem. Attila is a three-dimensional unstructured tetrahedral mesh discrete-ordinate code and was used for the three-dimensional problem. Both codes use discontinuous finite element spatial differencing. Both codes use diffusion synthetic acceleration (DSA) for accelerating the inner iterations.

  8. Heteronuclear three-dimensional NMR spectroscopy of the inflammatory protein C5a

    International Nuclear Information System (INIS)

    Zuiderweg, E.R.P.; Fesik, S.W.

    1989-01-01

    The utility of three-dimensional heteronuclear NMR spectroscopy for the assignment of 1 H and 15 N resonances of the inflammatory protein C5a (MW 8500), uniformly labeled with 15 N, is demonstrated at a protein concentration of 0.7 mM. It is shown that dramatic simplification of the 2D nuclear Overhauser effect spectrum (NOESY) is obtained by editing with respect to the frequency of the 15 N heteronucleus in a third dimension. The improved resolution in the 3D experiment largely facilitates the assignment of protein NMR spectra and allows for the determination of distance constraints from otherwise overlapping NOE cross peaks for purposes of 3D structure determination. The results show that 15 N heteronuclear 3D NMR can facilitate the structure determination of small proteins and promises to be a useful tool for the study of larger systems that cannot be studied by conventional 2D NMR techniques

  9. Heteronuclear three-dimensional NMR spectroscopy of the inflammatory protein C5a

    Energy Technology Data Exchange (ETDEWEB)

    Zuiderweg, E.R.P.; Fesik, S.W. (Abbott Laboratories, Abbott Park, IL (USA))

    1989-03-21

    The utility of three-dimensional heteronuclear NMR spectroscopy for the assignment of {sup 1}H and {sup 15}N resonances of the inflammatory protein C5a (MW 8500), uniformly labeled with {sup 15}N, is demonstrated at a protein concentration of 0.7 mM. It is shown that dramatic simplification of the 2D nuclear Overhauser effect spectrum (NOESY) is obtained by editing with respect to the frequency of the {sup 15}N heteronucleus in a third dimension. The improved resolution in the 3D experiment largely facilitates the assignment of protein NMR spectra and allows for the determination of distance constraints from otherwise overlapping NOE cross peaks for purposes of 3D structure determination. The results show that {sup 15}N heteronuclear 3D NMR can facilitate the structure determination of small proteins and promises to be a useful tool for the study of larger systems that cannot be studied by conventional 2D NMR techniques.

  10. Randomized Trial of C5a Receptor Inhibitor Avacopan in ANCA-Associated Vasculitis.

    Science.gov (United States)

    Jayne, David R W; Bruchfeld, Annette N; Harper, Lorraine; Schaier, Matthias; Venning, Michael C; Hamilton, Patrick; Burst, Volker; Grundmann, Franziska; Jadoul, Michel; Szombati, István; Tesař, Vladimír; Segelmark, Mårten; Potarca, Antonia; Schall, Thomas J; Bekker, Pirow

    2017-09-01

    Alternative C activation is involved in the pathogenesis of ANCA-associated vasculitis. However, glucocorticoids used as treatment contribute to the morbidity and mortality of vasculitis. We determined whether avacopan (CCX168), an orally administered, selective C5a receptor inhibitor, could replace oral glucocorticoids without compromising efficacy. In this randomized, placebo-controlled trial, adults with newly diagnosed or relapsing vasculitis received placebo plus prednisone starting at 60 mg daily (control group), avacopan (30 mg, twice daily) plus reduced-dose prednisone (20 mg daily), or avacopan (30 mg, twice daily) without prednisone. All patients received cyclophosphamide or rituximab. The primary efficacy measure was the proportion of patients achieving a ≥50% reduction in Birmingham Vasculitis Activity Score by week 12 and no worsening in any body system. We enrolled 67 patients, 23 in the control and 22 in each of the avacopan groups. Clinical response at week 12 was achieved in 14 of 20 (70.0%) control patients, 19 of 22 (86.4%) patients in the avacopan plus reduced-dose prednisone group (difference from control 16.4%; two-sided 90% confidence limit, -4.3% to 37.1%; P =0.002 for noninferiority), and 17 of 21 (81.0%) patients in the avacopan without prednisone group (difference from control 11.0%; two-sided 90% confidence limit, -11.0% to 32.9%; P =0.01 for noninferiority). Adverse events occurred in 21 of 23 (91%) control patients, 19 of 22 (86%) patients in the avacopan plus reduced-dose prednisone group, and 21 of 22 (96%) patients in the avacopan without prednisone group. In conclusion, C5a receptor inhibition with avacopan was effective in replacing high-dose glucocorticoids in treating vasculitis. Copyright © 2017 by the American Society of Nephrology.

  11. Interactive graphics analysis system for nuclear engineering applications

    International Nuclear Information System (INIS)

    Danchak, M.; Moyer, W.R.; Becker, M.

    1973-01-01

    From working with continuous slowing down theory, the need was recognized for a system which allowed rapid calculation of the theoretical flux, instant comparison with experiment and a simple means of iterating on the slowing down parameters to force flux agreement and reflect cross section modification. Similar requirements exist in other areas of nuclear work for streamlining and simplifying the data analysis process. As a solution, a unique interactive graphics analysis system (RIGAS) was devised to allow a user to calculate, display, compare, manipulate and modify his data without requiring any programming on his part. This was accomplished by establishing human primacy, through extensive human factor considerations, and designing a man-machine dialogue which responds to the mere push of a button. This system results in an instrument which maximizes man's decision making capability and the computer's speed to improve graphic communication and data analysis. (14 figs) (U.S.)

  12. Histochemical response of `Grande naine' plants inoculated with M. fijiensis and Bacillus pumilus CCIBP-C5 cell free filtrate

    Directory of Open Access Journals (Sweden)

    Eilyn Mena

    2015-04-01

    Full Text Available In recent years, interest has won biocontrol Mycosphaerella fijiensis with the use of microorganisms or their products. Nevertheless, the knowledge of the biochemical events involved in Musa- M. fijiensis-microorganism interaction, is still limited. The objective was to determine the histochemical response of `Grande naine' (Musa AAA plants inoculated or not with the pathogen in the presence of culture filtrate of Bacillus pumilus CCIBP-C5. For this, techniques that allow visualization of callose, superoxide anion, lignin and phenolic compounds in the early days post inoculation (dpi were used. As a result, at 6 dpi callose deposition were observed within the stomata in plants where the culture filtrate was applied regardless of the presence or absence of the pathogen. Furthermore, a blue halo was observed around stomas, indicative of the presence of superoxide anion. It was not possible to detect accumulation of lignin or phenolic compounds with the technique used. It was demonstrated the accumulation of biochemical compounds related to defense response of `Grande naine' plants to pathogen infection. Furthermore, it was shown that the plant responds in early infection with the use of bacterial culture filtrates. These results demonstrate the possible mechanism of induction of defense response in plants of Musa sp. in the presence of bacterial culture filtrate. Key words: Black Sigatoka, callose, lignin, phenols, superoxide anion

  13. Impact of the surgical strategy on the incidence of C5 nerve root palsy in decompressive cervical surgery.

    Directory of Open Access Journals (Sweden)

    Theresa Krätzig

    Full Text Available Our aim was to identify the impact of different surgical strategies on the incidence of C5 palsy.Degenerative cervical spinal stenosis is a steadily increasing morbidity in the ageing population. Postoperative C5 nerve root palsy is a common complication with severe impact on the patients´ quality of life.We identified 1708 consecutive patients who underwent cervical decompression surgery due to degenerative changes. The incidence of C5 palsy and surgical parameters including type and level of surgery were recorded to identify predictors for C5 nerve palsy.The overall C5 palsy rate was 4.8%, with 18.3% of cases being bilateral. For ACDF alone the palsy rate was low (1.13%, compared to 14.0% of C5 palsy rate after corpectomy. The risk increased with extension of the procedures. Hybrid constructs with corpectomy plus ACDF at C3-6 showed significantly lower rates of C5 palsy (10.7% than corpectomy of two vertebrae (p = 0.005. Multiple regression analysis identified corpectomy of C4 or C5 as a significant predictor. We observed a lower overall incidence for ventral (4.3% compared to dorsal (10.9% approaches (p<0.001. When imaging detected a postoperative shift of the spinal cord at index segment C4/5, palsy rate increased significantly (33.3% vs. 12.5%, p = 0.034.Extended surgical strategies, such as dorsal laminectomies, multilevel corpectomies and procedures with extensive spinal cord shift were shown to display a high risk of C5 palsy. The use of extended procedures should therefore be employed cautiously. Switching to combined surgical methods like ACDF plus corpectomy can reduce the rate of C5 palsy.

  14. Evaluation of SF6-alternative gas C5-PFK based on arc extinguishing performance and electric strength

    Science.gov (United States)

    Wu, Yi; Wang, Chunlin; Sun, Hao; Rong, Mingzhe; Murphy, Anthony Bruce; Li, Tianwei; Zhong, Jianying; Chen, Zhexin; Yang, Fei; Niu, Chunpin

    2017-09-01

    C5-PFK (C5-perfluoroketone, C5F10O) is under wide consideration as an environmentally-friendly alternative gas to SF6 in high-voltage applications, because of its superior insulation performance. The aim of this work is to study theoretically the arc extinguishing performance and electric strength of C5-PFK. The arc extinguishing performance of C5-PFK was evaluated by analyzing and comparing the thermophysical properties of C5-PFK, SF6, CF4, CO2 and N2 plasmas. It was difficult to obtain the species formed in C5-PFK plasmas because of the complex C5-PFK molecular decomposition process. In this work, the decomposition process of C5-PFK and the related species were analyzed by the bond energy analysis method. For the species for which parameters such as the partition function and the enthalpy of formation were not available, computational chemistry methods were used to obtain the required data. The collision integrals were calculated using the phenomenological potential model. Using these results, the local thermodynamic equilibrium composition at temperatures from 300 to 30 000 K at 1-10 atm of pure C5-PFK was calculated by the method of minimization of the Gibbs free energy, and the corresponding transport coefficients were calculated by Chapman-Enskog method. Through the comparison of the thermophysical properties, it was found that C5-PFK had similar characteristics to SF6, with large peaks in specific heat below 4500 K, indicating potentially good thermal interruption capability. However, the specific heat peak at 7000 K corresponding to CO decomposition may detract from the thermal interruption capability. Specific heat peaks at higher temperatures are associated with the breaking of double or triple bonds, and should be avoided if possible in the new alternative gases. The electric strength of C5-PFK was assessed using the molecular electrostatic potential, which can be accurately calculated or measured, and gives strong insights into important

  15. Calculation of thermodynamic properties and transport coefficients of C5F10O-CO2 thermal plasmas

    Science.gov (United States)

    Li, Xingwen; Guo, Xiaoxue; Murphy, Anthony B.; Zhao, Hu; Wu, Jian; Guo, Ze

    2017-10-01

    The thermodynamic properties and transport coefficients of C5F10O-CO2 gas mixtures, which are being considered as substitutes for SF6 in circuit breaker applications, are calculated for the temperature range from 300 K to 30 000 K and the pressure range from 0.05 MPa to 1.6 MPa. Special attention is paid on investigating the evolution of thermophysical properties of C5F10O-CO2 mixtures with different mixing ratios and with different pressures; both the mixing ratio and pressure significantly affect the properties. This is explained mainly in terms of the changes in the temperatures at which the dissociation and ionization reactions take place. Comparisons of different thermophysical properties of C5F10O-CO2 mixtures with those of SF6 are also carried out. It is found that most of the thermophysical properties of the C5F10O-CO2 mixtures, such as thermal conductivity, viscosity, and electrical conductivity, become closer to those of SF6 as the C5F10O concentration increases. The composition and thermophysical properties of pure C5F10O in the temperature range from 300 K to 2000 K based on the decomposition pathway are also given. The calculation results provide a basis for further study of the insulation and arc-quenching capability of C5F10O-CO2 gas mixtures as substitutes for SF6.

  16. Purification of the active C5a receptor from human polymorphonuclear leukocytes as a receptor - G sub i complex

    Energy Technology Data Exchange (ETDEWEB)

    Rollins, T.E.; Siciliano, S.; Kobayashi, S.; Cianciarulo, D.N.; Bonilla-Argudo, V.; Collier, K.; Springer, M.S. (Merck Sharp and Dohme Research Lab., Rahway, NJ (United States))

    1991-02-01

    The authors have isolated, in an active state, the C5a receptor from human polymorphonuclear leukocytes. The purification was achieved in a single step using a C5a affinity column in which the C5a molecule was coupled to the resin through its N terminus. The purified receptor, like the crude solubilized molecule, exhibited a single class of high-affinity binding sites with a K{sub d} of 30 pM. Further, the binding of C5a retained its sensitivity to guanine nucleotides, implying that the purified receptor contained a guanine nucleotide-binding protein (G protein). SDS/PAGE revealed the presence of three polypeptides with molecular masses of 42, 40, and 36 kDa, which were determined to be the C5a-binding subunit and the {alpha} and {beta} subunits of G{sub i}, respectively. The 36- and 40-kDa polypeptides were identified by immunoblotting and by the ability of pertussis toxin to ADP-ribosylate the 40-kDa molecule. These results confirm their earlier hypothesis that the receptor exists as a complex with a G protein in the presence or absence of C5a. The tight coupling between the receptor and G protein should make possible the identification of the G protein(s) involved in the transduction pathways used by C5a to produce its many biological effects.

  17. Purification of the active C5a receptor from human polymorphonuclear leukocytes as a receptor - Gi complex

    International Nuclear Information System (INIS)

    Rollins, T.E.; Siciliano, S.; Kobayashi, S.; Cianciarulo, D.N.; Bonilla-Argudo, V.; Collier, K.; Springer, M.S.

    1991-01-01

    The authors have isolated, in an active state, the C5a receptor from human polymorphonuclear leukocytes. The purification was achieved in a single step using a C5a affinity column in which the C5a molecule was coupled to the resin through its N terminus. The purified receptor, like the crude solubilized molecule, exhibited a single class of high-affinity binding sites with a K d of 30 pM. Further, the binding of C5a retained its sensitivity to guanine nucleotides, implying that the purified receptor contained a guanine nucleotide-binding protein (G protein). SDS/PAGE revealed the presence of three polypeptides with molecular masses of 42, 40, and 36 kDa, which were determined to be the C5a-binding subunit and the α and β subunits of G i , respectively. The 36- and 40-kDa polypeptides were identified by immunoblotting and by the ability of pertussis toxin to ADP-ribosylate the 40-kDa molecule. These results confirm their earlier hypothesis that the receptor exists as a complex with a G protein in the presence or absence of C5a. The tight coupling between the receptor and G protein should make possible the identification of the G protein(s) involved in the transduction pathways used by C5a to produce its many biological effects

  18. Comparing the Effectiveness of Sagittal Balance, Foraminal Stenosis, and Preoperative Cord Rotation in Predicting Postoperative C5 Palsy.

    Science.gov (United States)

    Chugh, Arunit J S; Weinberg, Douglas S; Alonso, Fernando; Eubanks, Jason D

    2017-11-01

    Retrospective cohort review. To determine whether preoperative cord rotation is independently correlated with C5 palsy when analyzed alongside measures of sagittal balance and foraminal stenosis. Postoperative C5 palsy is a well-documented complication of cervical procedures with a prevalence of 4%-8%. Recent studies have shown a correlation with preoperative spinal cord rotation. There have been few studies, however, that have examined the role of sagittal balance and foraminal stenosis in the development of C5 palsy. A total of 77 patients who underwent cervical decompression-10 of whom developed C5 palsy-were reviewed. Sagittal balance was assessed using curvature angle and curvature index on radiographs and magnetic resonance image (MRI). Cord rotation was assessed on axial MRI. C4-C5 foraminal stenosis was assessed on sagittal MRI using area measurements and a grading scale. Demographics and information on surgical approach were gathered from chart review. Correlation with C5 palsy was performed by point-biserial, χ, and regression analyses. Point-biserial analysis indicated that only cord rotation showed significance (Pbalance did not correlate with presence of C5 palsy. Logistic regression model yielded cord rotation as the only significant independent predictor of C5 palsy. For every degree of axial cord rotation, the likelihood ratio for suffering a C5 palsy was 3.93 (95% confidence interval, 2.01-8.66; Ppoints to mechanisms other than direct compression as the etiology. In addition, the lack of correlation with postoperative changes in sagittal balance hints that measures of curvature angle and curvature index may not be appropriate to accurately predict this complication. Level 3.

  19. C5 glycolipids of heterocystous cyanobacteria track symbiont abundance in the diatom Hemiaulus hauckii across the tropical North Atlantic

    Science.gov (United States)

    Bale, Nicole J.; Villareal, Tracy A.; Hopmans, Ellen C.; Brussaard, Corina P. D.; Besseling, Marc; Dorhout, Denise; Sinninghe Damsté, Jaap S.; Schouten, Stefan

    2018-03-01

    Diatom-diazotroph associations (DDAs) include marine heterocystous cyanobacteria found as exosymbionts and endosymbionts in multiple diatom species. Heterocysts are the site of N2 fixation and have thickened cell walls containing unique heterocyst glycolipids which maintain a low oxygen environment within the heterocyst. The endosymbiotic cyanobacterium Richelia intracellularis found in species of the diatom genus Hemiaulus and Rhizosolenia makes heterocyst glycolipids (HGs) which are composed of C30 and C32 diols and triols with pentose (C5) moieties that are distinct from limnetic cyanobacterial HGs with predominantly hexose (C6) moieties. Here we applied a method for analysis of intact polar lipids to the study of HGs in suspended particulate matter (SPM) and surface sediment from across the tropical North Atlantic. The study focused on the Amazon plume region, where DDAs are documented to form extensive surface blooms, in order to examine the utility of C5 HGs as markers for DDAs as well as their transportation to underlying sediments. C30 and C32 triols with C5 pentose moieties were detected in both marine SPM and surface sediments. We found a significant correlation between the water column concentration of these long-chain C5 HGs and DDA symbiont counts. In particular, the concentrations of both the C5 HGs (1-(O-ribose)-3,27,29-triacontanetriol (C5 HG30 triol) and 1-(O-ribose)-3,29,31-dotriacontanetriol (C5 HG32 triol)) in SPM exhibited a significant correlation with the number of Hemiaulus hauckii symbionts. This result strengthens the idea that long-chain C5 HGs can be applied as biomarkers for marine endosymbiotic heterocystous cyanobacteria. The presence of the same C5 HGs in surface sediment provides evidence that they are effectively transported to the sediment and hence have potential as biomarkers for studies of the contribution of DDAs to the paleo-marine N cycle.

  20. 3-D extension C5G7 MOX benchmark results using PARTISN

    Energy Technology Data Exchange (ETDEWEB)

    Dahl, J.A. [Los Alamos National Laboratory, CCS-4 Transport Methods Group, Los Alamos, NM (United States)

    2005-07-01

    We have participated in the Expert Group of 3-D Radiation Transport Benchmarks' proposed 3-dimensional Extension C5G7 MOX problems using the discrete ordinate transport code PARTISN. The computational mesh was created using the FRAC-IN-THE-BOX code, which produces a volume fraction Cartesian mesh from combinatorial geometry descriptions. k{sub eff} eigenvalues, maximum pin powers, and average fuel assembly powers are reported and compared to a benchmark quality Monte Carlo solution. We also present a two dimensional mesh convergence study examining the affects of using volume fractions to approximate the water-pin cell interface. It appears that the control rod pin cell must be meshed twice as fine as a fuel pin cell in order to achieve the same spatial error when using the volume fraction method to define water channel-pin cell interfaces. It is noted that the previous PARTISN results provided to the OECD/NEA Expert Group on 3-dimensional Radiation Benchmarks contained a cross section error, and therefore should be disregarded.

  1. 3-D extension C5G7 MOX benchmark results using PARTISN

    International Nuclear Information System (INIS)

    Dahl, J.A.

    2005-01-01

    We have participated in the Expert Group of 3-D Radiation Transport Benchmarks' proposed 3-dimensional Extension C5G7 MOX problems using the discrete ordinate transport code PARTISN. The computational mesh was created using the FRAC-IN-THE-BOX code, which produces a volume fraction Cartesian mesh from combinatorial geometry descriptions. k eff eigenvalues, maximum pin powers, and average fuel assembly powers are reported and compared to a benchmark quality Monte Carlo solution. We also present a two dimensional mesh convergence study examining the affects of using volume fractions to approximate the water-pin cell interface. It appears that the control rod pin cell must be meshed twice as fine as a fuel pin cell in order to achieve the same spatial error when using the volume fraction method to define water channel-pin cell interfaces. It is noted that the previous PARTISN results provided to the OECD/NEA Expert Group on 3-dimensional Radiation Benchmarks contained a cross section error, and therefore should be disregarded

  2. Evidence for an anisotropic contact shift. Proton NMR study of line shapes in uranocene and (C5H5)3UCl powders

    International Nuclear Information System (INIS)

    McGarvey, B.R.; Nagy, S.

    1987-01-01

    The proton NMR spectra of solid powders of uranocene and (C 5 H 5 ) 3 UCl were measured from 90 to 298 K. The line shapes of both systems became increasingly anisotropic as the temperature was lowered. The cyclooctatetraene rings in uranocene were found to be rotating at a frequency greater than 100 kHz down to 90 K. The (C 5 H 5 ) 3 UCl molecules were found to be reorienting rapidly above 220 K, but below 140 K the NMR spectra were characteristic of a rigid lattice with no rotation of the cyclopentadienyl rings. The spectra of both compounds could be simulated by assuming an axial paramagnetic shift tensor and an orientation-dependent line width. Comparison of the experimental shift tensor with that calculated for a point dipolar interaction revealed a large and very anisotropic paramagnetic shift for uranocene due to unpaired spin transferred into the ligand orbitals. The shift was large when the magnetic field was along the 8-fold symmetry axis of the molecule and nearly zero perpendicular to the axis. It appears conclusive that the contact shift in uranocene is not isotropic at all. A similar anisotropy in the contact shift associated with the cyclopentadienyl rings is evident also in the results for (C 5 H 5 )UCl. The average solid-state shift of uranocene agreed with the solution shift, within experimental error, but the solid state shift of (C 5 H 5 ) 3 UCl was 42 ppm greater than the solution shift at 298 K, indicating a difference in molecular geometry between the crystalline state and solution. 32 references, 8 figures, 3 tables

  3. Synergistic enhancement of chemokine generation and lung injury by C5a or the membrane attack complex of complement

    DEFF Research Database (Denmark)

    Czermak, B J; Lentsch, A B; Bless, N M

    1999-01-01

    demonstrated synergistic production of C-X-C (macrophage inflammatory protein-2 and cytokine-induced neutrophil chemoattractant) and C-C (macrophage inflammatory protein-1alpha and monocyte chemoattractant-1) chemokines. In the absence of the costimulus, C5a or MAC did not induce chemokine generation....... In in vivo studies, C5a and MAC alone caused limited or no intrapulmonary generation of chemokines, but in the presence of a costimulus (IgG immune complexes) C5a and MAC caused synergistic intrapulmonary generation of C-X-C and C-C chemokines but not of tumor necrosis factor alpha. Under these conditions...... increased neutrophil accumulation occurred, as did lung injury. These observations suggest that C5a and MAC function synergistically with a costimulus to enhance chemokine generation and the intensity of the lung inflammatory response....

  4. Combustion Characteristics of C5 Alcohols and a Skeletal Mechanism for Homogeneous Charge Compression Ignition Combustion Simulation

    KAUST Repository

    Park, Sungwoo

    2015-10-27

    C5 alcohols are considered alternative fuels because they emit less greenhouse gases and fewer harmful pollutants. In this study, the combustion characteristics of 2-methylbutanol (2-methyl-1-butanol) and isopentanol (3-methyl-1-butanol) and their mixtures with primary reference fuels (PRFs) were studied using a detailed chemical kinetic model obtained from merging previously published mechanisms. Ignition delay times of the C5 alcohol/air mixtures were compared to PRFs at 20 and 40 atm. Reaction path analyses were conducted at intermediate and high temperatures to identify the most influential reactions controlling ignition of C5 alcohols. The direct relation graph with expert knowledge methodology was used to eliminate unimportant species and reactions in the detailed mechanism, and the resulting skeletal mechanism was tested at various homogeneous charge compression ignition (HCCI) engine combustion conditions. These simulations were used to investigate the heat release characteristics of the methyl-substituted C5 alcohols, and the results show relatively strong reactions at intermediate temperatures prior to hot ignition. C5 alcohol blending in PRF75 in HCCI combustion leads to a significant decrease of low-temperature heat release (LTHR) and a delay of the main combustion. The heat release features demonstrated by C5 alcohols can be used to improve the design and operation of advanced engine technologies.

  5. The H_6_0Si_6C_5_4 heterofullerene as high-capacity hydrogen storage medium

    International Nuclear Information System (INIS)

    Yong, Yongliang; Zhou, Qingxiao; Li, Xiaohong; Lv, Shijie

    2016-01-01

    With the great success in Si atoms doped C_6_0 fullerene and the well-established methods for synthesis of hydrogenated carbon fullerenes, this leads naturally to wonder whether Si-doped fullerenes are possible for special applications such as hydrogen storage. Here by using first-principles calculations, we design a novel high-capacity hydrogen storage material, H_6_0Si_6C_5_4 heterofullerene, and confirm its geometric stability. It is found that the H_6_0Si_6C_5_4 heterofullerene has a large HOMO-LUMO gap and a high symmetry, indicating it is high chemically stable. Further, our finite temperature simulations indicate that the H_6_0Si_6C_5_4 heterofullerene is thermally stable at 300 K. H_2 molecules would enter into the cage from the Si-hexagon ring because of lower energy barrier. Through our calculation, a maximum of 21 H_2 molecules can be stored inside the H_6_0Si_6C_5_4 cage in molecular form, leading to a gravimetric density of 11.11 wt% for 21H_2@H_6_0Si_6C_5_4 system, which suggests that the hydrogenated Si_6C_5_4 heterofullerene could be suitable as a high-capacity hydrogen storage material.

  6. Tissue Destruction in Bullous Pemphigoid Can Be Complement Independent and May Be Mitigated by C5aR2

    Directory of Open Access Journals (Sweden)

    Christian M. Karsten

    2018-03-01

    Full Text Available Bullous pemphigoid (BP, the most frequent autoimmune bullous disorder, is a paradigmatic autoantibody-mediated disease associated with autoantibodies against BP180 (type XVII collagen, Col17. Several animal models have been developed that reflect important clinical and immunological features of human BP. Complement activation has been described as a prerequisite for blister formation, however, the recent finding that skin lesions can be induced by anti-Col17 F(ab′2 fragments indicates complement-independent mechanisms to contribute to blister formation in BP. Here, C5−/− mice injected with anti-Col17 IgG showed a reduction of skin lesions by about 50% associated with significantly less skin-infiltrating neutrophils compared to wild-type mice. Reduction of skin lesions and neutrophil infiltration was seen independently of the employed anti-Col17 IgG dose. Further, C5ar1−/− mice were protected from disease development, whereas the extent of skin lesions was increased in C5ar2−/− animals. Pharmacological inhibition of C5a receptor 1 (C5aR1 by PMX53 led to reduced disease activity when applied in a prophylactic setting. In contrast, PMX-53 treatment had no effect when first skin lesions had already developed. While C5aR1 was critically involved in neutrophil migration in vitro, its role for Col17-anti-Col17 IgG immune complex-mediated release of reactive oxygen species from neutrophils was less pronounced. Our data demonstrate that complement-dependent and -independent mechanisms coexist in anti-Col17-autoantibody-mediated tissue destruction. C5aR1 and C5aR2 seem to play opposing roles in this process with C5aR1 exerting its primary effect in recruiting inflammatory cells to the skin during the early phase of the disease. Further studies are required to fully understand the role of C5aR2 in autoantibody-mediated skin inflammation.

  7. Human machine interaction research experience and perspectives as seen from the OECD Halden Reactor Project

    International Nuclear Information System (INIS)

    Oewre, F.

    1999-01-01

    In this paper a short review is given on important safety issues in the field of human machine interaction as expressed by important nuclear organisations such as USNRC, IAEA and the OECD NEA. Further on, a presentation is offered of research activities at the OECD Halden Reactor Project in the field of human machine interaction aiming to clarify some of the issues outlined by the above mentioned organisations. The OECD Halden Reactor Project is a joint undertaking of national nuclear organisations in 19 countries sponsoring a jointly financed research programme under the auspices of the OECD - Nuclear Energy Agency. One of the research areas is the man-machine systems research addressing the operator tasks in a control room environment. The overall objective is to provide a basis for improving today's control rooms through introduction of computer-based solutions for effective and safe execution of surveillance and control functions in normal as well as off-normal plant situations. (author)

  8. C5a regulates IL-12+ DC migration to induce pathogenic Th1 and Th17 cells in sepsis.

    Directory of Open Access Journals (Sweden)

    Ning Ma

    Full Text Available OBJECTIVE: It is well known that complement system C5a is excessively activated during the onset of sepsis. However, it is unclear whether C5a can regulate dentritic cells (DCs to stimulate adaptive immune cells such as Th1 and Th17 in sepsis. METHODS: Sepsis was induced by cecal ligation and puncture (CLP. CLP-induced sepsis was treated with anti-C5a or IL-12. IL-12(+DC, IFNγ(+Th1, and IL-17(+Th17 cells were analyzed by flow cytometry. IL-12 was measured by ELISA. RESULTS: Our studies here showed that C5a induced IL-12(+DC cell migration from the peritoneal cavity to peripheral blood and lymph nodes. Furthermore, IL-12(+DC cells induced the expansion of pathogenic IFNγ(+Th1 and IL-17(+Th17 cells in peripheral blood and lymph nodes. Moreover, IL-12, secreted by DC cells in the peritoneal cavity, is an important factor that prevents the development of sepsis. CONCLUSION: Our data suggests that C5a regulates IL-12(+DC cell migration to induce pathogenic Th1 and Th17 cells in sepsis.

  9. Atomic-scale microstructures of Zr2Al3C4 and Zr3Al3C5 ceramics

    International Nuclear Information System (INIS)

    Lin, Z.J.; Zhuo, M.J.; He, L.F.; Zhou, Y.C.; Li, M.S.; Wang, J.Y.

    2006-01-01

    The microstructures of bulk Zr 2 Al 3 C 4 and Zr 3 Al 3 C 5 ceramics have been investigated using transmission electron microscopy and scanning transmission electron microscopy. These two carbides were determined to have a point group 6/mmm and a space group P6 3 /mmc using selected-area electron diffraction and convergent beam electron diffraction. The atomic-scale microstructures of Zr 2 Al 3 C 4 and Zr 3 Al 3 C 5 were investigated through high-resolution imaging and Z-contrast imaging. Furthermore, intergrowth between Zr 2 Al 3 C 4 and Zr 3 Al 3 C 5 was identified. Stacking faults in Zr 3 Al 3 C 5 were found to result from the insertion of an additional Zr-C layer. Cubic ZrC was occasionally identified to be incorporated in elongated Zr 3 Al 3 C 5 grains. In addition, Al may induce a twinned ZrC structure and lead to the formation of ternary zirconium aluminum carbides

  10. Penta-SiC5 monolayer: A novel quasi-planar indirect semiconductor with a tunable wide band gap

    Science.gov (United States)

    Naseri, Mosayeb

    2018-03-01

    In this paper, by using of the first principles calculations in the framework of the density functional theory, we systematically investigated the structure, stability, electronic and optical properties of a novel two-dimensional pentagonal monolayer semiconductors namely penta-SiC5 monolayer. Comparing elemental silicon, diamond, and previously reported 2D carbon allotropes, our calculation shows that the predicted penta-SiC5 monolayer has a metastable nature. The calculated results indicate that the predicted monolayer is an indirect semiconductor with a wide band gap of about 2.82 eV by using Heyd-Scuseria-Ernzerhof (HSE06) hybrid functional level of theory which can be effectively tuned by external biaxial strains. The obtained exceptional electronic properties suggest penta-SiC5 monolayer as promising candidates for application in new electronic devices in nano scale.

  11. Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection.

    Science.gov (United States)

    Hudault, S; Liévin, V; Bernet-Camard, M F; Servin, A L

    1997-02-01

    The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invading strain C5 and either the culture or the supernatant of L. casei GG impeded the invasion by the Salmonella strain in Caco-2 cells, without modifying the viability of the strain. After neutralization at pH 7, no inhibition of the invasion by C5 was observed. The antagonistic activity of L. casei GG was examined in C3H/He/Oujco mice orally infected with C5 as follows: (i) L. casei GG was given daily to conventional animals as a probiotic, and (ii) it was given once to germ-free animals in order to study the effect of the population of L. casei GG established in the different segments of the gut. In vivo experiments show that after a single challenge with C5, this strain survives and persists at a higher level in the feces of the untreated conventional mice than in those of the treated group. In L. casei GG germ-free mice, establishment of L. casei GG in the gut significantly delayed the occurrence of 100% mortality of the animals (15 days after C5 challenge versus 9 days in germ-free mice [P L. casei GG population level in the gut dramatically decreased in these animals.

  12. Indomethacin inhibits the increased airway responsiveness to histamine following inhalation of C5a des Arg in rabbits.

    Science.gov (United States)

    Berend, N; Armour, C L; Black, J L

    1986-08-01

    It has been shown that inhalation of C5a des Arg increases rabbit airway responsiveness to histamine and that this is associated with an influx of neutrophils into the airway walls. This study was undertaken to see if the augmented response to histamine can be blocked by the cyclo-oxygenase inhibitor indomethacin. Spontaneously breathing, anesthetised rabbits were studied in a volume displacement plethysmograph and pulmonary resistance (R1) was measured using the electrical subtraction technique. Histamine does response curves (HDR) were generated by measuring R1 after serial nubulisation of saline and histamine (1, 3, 10, 30 and 100 mg/ml). Aerosols of either saline or C5a des Arg (1.5 ug/ml) were then inhaled by the animals over a time period of 2 min. An HDR was then repeated 4 hours later. In 9 rabbits the inhalation of C5a des Arg resulted in an upward shift of the repeat HDR: the area under the HDR was significantly greater than under the first HDR (p less than 0.05). In 6 rabbits the repeat HDR 4 hours after saline was shifted downwards (N.S.) indicating some degree of tachyphylaxis. When rabbits were pretreated with indomethacin (5 mg/kg i.v.) the repeat HDR following either C5a des Arg (n = 7) or saline (n = 6) were also shifted downwards i.e., the increased airway responsiveness noted after C5a des Arg was abolished. There was no significant difference in baseline saline R1 during the first or second HDR in any group. These results suggest that the increased airway responsiveness following nebulisation of C5a des Arg may be due to release from neutrophils of products of the cyclo-oxygenase pathway.

  13. Sequence-specific assignments in the 1H NMR spectrum of the human inflammatory protein C5a

    International Nuclear Information System (INIS)

    Zuiderweg, E.R.P.; Mollison, K.W.; Henkin, J.; Carter, G.W.

    1988-01-01

    Full sequence-specific assignments for the 1 H NMR lines of the backbone protons of the human complement factor C5a are described and documented. The results were obtained by largely following the methodology developed by Wuethrich et al. Assignments for the majority of the amino acid side chain protons were obtained by using a comparison of double- and triple-quantum-filtered two-dimensional correlated experiments together with the analysis of relayed coherence transfer spectra. The assignments provide the basis for the determination of the thus far unknown three-dimensional structure of C5a from nuclear Overhauser enhancement distance constraints

  14. Autoradiographic studies on the distribution of 14C-5,7-dihydroxytryptamine in the brain of new-born rat

    International Nuclear Information System (INIS)

    Lappe, U.

    1982-01-01

    The distribution of intracisternally injected 14 C-5,7-dihydroxy tryptamine (5,7-DHT) in the central nervous system of new-born rat is studied by means of autoradiography. The radio-active neurotoxin is incorporated into the neurones of all known serotonine nucleus groups. This labelling allows a detailed demonstration of the topography of the serotonine neurones in the brain stem of the new-born rat and to compare it with systems obtained by other methods. Serotonine neurones were mapped in 22 representative frontal sections through the brain stem. 14 C-5,7-DHT is incorporated into noradrenergic neurones, too. However, labelling is less marked than in serotonergic neurones. 14 C-5,7-DHT is incorporated at small quantities into the following extraneural elements: fibroblasts of the pia mater/arachnoidea, some endothelical cells of pial vessels, epithelial cells of the plexus choroideus, and subependymal macrophages. 6 h after injection of 25 μg 14 C-5,7-DHT, the vast majority of serotonergic neurones reveal strong degenerative changes which are irreversible. (orig./MG) [de

  15. Compromised neutrophil function and severe bovine E.coli mastitis: is C5a the missing link?

    Science.gov (United States)

    Around the periparturient period and during early lactation dairy cows have an elevated risk for clinical mastitis. The severity of Gram-negative infections during these periods has been correlated with reduced neutrophil functions. In this review we focus on the potential role of C5a in the develop...

  16. Development of a mathematical model describing hydrolysis and co-fermentation of C6 and C5 sugars

    DEFF Research Database (Denmark)

    Morales Rodriguez, Ricardo; Gernaey, Krist; Meyer, Anne S.

    2010-01-01

    saccharification and co-fermentation (SSCF) of C6 and C5 sugars. Model construction has been carried out by combining existing mathematical models for enzymatic hydrolysis on the one hand and co-fermentation on the other hand. An inhibition of ethanol on cellulose conversion was introduced in order to increase...

  17. Combustion Characteristics of C5 Alcohols and a Skeletal Mechanism for Homogeneous Charge Compression Ignition Combustion Simulation

    KAUST Repository

    Park, Sungwoo; Chung, Suk-Ho; Lu, Tianfeng; Sarathy, Mani

    2015-01-01

    ) and their mixtures with primary reference fuels (PRFs) were studied using a detailed chemical kinetic model obtained from merging previously published mechanisms. Ignition delay times of the C5 alcohol/air mixtures were compared to PRFs at 20 and 40 atm. Reaction

  18. A new adsorption isotherm for C5 hydrocarbons on metal–organic framework Cu3(BTC)2

    Czech Academy of Sciences Publication Activity Database

    Zukal, Arnošt; Kubů, Martin

    2015-01-01

    Roč. 21, 1-2 (2015), s. 99-105 ISSN 0929-5607 R&D Projects: GA ČR GA14-07101S Institutional support: RVO:61388955 Keywords : Cu3(BTC)2 * adsorption * C5 hydrocarbons Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.870, year: 2015

  19. Improving medical diagnosis reliability using Boosted C5.0 decision tree empowered by Particle Swarm Optimization.

    Science.gov (United States)

    Pashaei, Elnaz; Ozen, Mustafa; Aydin, Nizamettin

    2015-08-01

    Improving accuracy of supervised classification algorithms in biomedical applications is one of active area of research. In this study, we improve the performance of Particle Swarm Optimization (PSO) combined with C4.5 decision tree (PSO+C4.5) classifier by applying Boosted C5.0 decision tree as the fitness function. To evaluate the effectiveness of our proposed method, it is implemented on 1 microarray dataset and 5 different medical data sets obtained from UCI machine learning databases. Moreover, the results of PSO + Boosted C5.0 implementation are compared to eight well-known benchmark classification methods (PSO+C4.5, support vector machine under the kernel of Radial Basis Function, Classification And Regression Tree (CART), C4.5 decision tree, C5.0 decision tree, Boosted C5.0 decision tree, Naive Bayes and Weighted K-Nearest neighbor). Repeated five-fold cross-validation method was used to justify the performance of classifiers. Experimental results show that our proposed method not only improve the performance of PSO+C4.5 but also obtains higher classification accuracy compared to the other classification methods.

  20. Effects of micro-water on decomposition of the environment-friendly insulating medium C5F10O

    Directory of Open Access Journals (Sweden)

    Song Xiao

    2017-06-01

    Full Text Available SF6 is widely used in all kinds of high-voltage electrical equipment because of its excellent insulation and arc-extinguishing performance. However, this compound leads to serious greenhouse effect, which harms the environment. Many research institutions are now actively in search of SF6 alternative gas. C5F10O has attracted much attention as an alternative gas with low global warming potential (GWP and excellent dielectric strength. In this paper, we analyzed the possible decomposition paths of C5F10O under micro-water environment through density functional theory. We also evaluated the ionization parameters and toxicity of the decomposition products. The results show that OH• and H• produced by H2O exhibited a catalytic effect on the decomposition of C5F10O. CF4, C2F6, C3F6, C3F8, C4F10, C5F12, C6F14, C3F7COH, C3F7OH, CF3COH, C3F7H, and CF3OH were produced in the micro-water environment. Based on molecular configuration calculation, the ionization parameters of these products were inferior to perfluorocarbons, such as C3F8, leading to reduced insulation performance of the system. Moreover, CF2O and HF are hazardous to human health and equipment safety. Results will provide a basis for further study of the insulation characteristic of the C5F10O gas mixture under micro-water condition to guide the formulation of their relevant international standards prior to engineering applications.

  1. Effects of micro-water on decomposition of the environment-friendly insulating medium C5F10O

    Science.gov (United States)

    Xiao, Song; Li, Yi; Zhang, Xiaoxing; Tian, Shuangshuang; Deng, Zaitao; Tang, Ju

    2017-06-01

    SF6 is widely used in all kinds of high-voltage electrical equipment because of its excellent insulation and arc-extinguishing performance. However, this compound leads to serious greenhouse effect, which harms the environment. Many research institutions are now actively in search of SF6 alternative gas. C5F10O has attracted much attention as an alternative gas with low global warming potential (GWP) and excellent dielectric strength. In this paper, we analyzed the possible decomposition paths of C5F10O under micro-water environment through density functional theory. We also evaluated the ionization parameters and toxicity of the decomposition products. The results show that OH• and H• produced by H2O exhibited a catalytic effect on the decomposition of C5F10O. CF4, C2F6, C3F6, C3F8, C4F10, C5F12, C6F14, C3F7COH, C3F7OH, CF3COH, C3F7H, and CF3OH were produced in the micro-water environment. Based on molecular configuration calculation, the ionization parameters of these products were inferior to perfluorocarbons, such as C3F8, leading to reduced insulation performance of the system. Moreover, CF2O and HF are hazardous to human health and equipment safety. Results will provide a basis for further study of the insulation characteristic of the C5F10O gas mixture under micro-water condition to guide the formulation of their relevant international standards prior to engineering applications.

  2. 40 CFR Table C-5 to Subpart C of... - Summary of Comparability Field Testing Campaign Site and Seasonal Requirements for Class II and...

    Science.gov (United States)

    2010-07-01

    ... Campaign Site and Seasonal Requirements for Class II and III FEMs for PM10â2.5 and PM2.5 C Table C-5 to Subpart C of Part 53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Between Candidate Methods and Reference Methods Pt. 53, Subpt. C, Table C-5 Table C-5 to Subpart C of Part...

  3. Identification of a Marine Bacillus Strain C5 and Parathion-Methyl Degradation Characteristics of the Extracellular Esterase B1

    Directory of Open Access Journals (Sweden)

    Jianhua Hao

    2014-01-01

    Full Text Available A bacterial strain C5 that can produce new type of marine esterase was isolated and screened from marine sludge. According to 16S rRNA sequence analysis and physiological and biochemical experiments, the strain was identified as Bacillus subtilis. A single isozyme with a molecular weight of 86 kDa was observed by SDS-PAGE and native-PAGE. On this basis, the mechanism of esterase B1 secreted by strain C5 degrading parathion-methyl was explored, and the effects of temperature and pH on the degradation rate were investigated. From the results, p-nitrophenol was one of the degradation products of B1 degrading parathion-methyl, and the best degradation effect could be achieved at the temperature of 40°C and the neutral pH value.

  4. Roles for C-X-C chemokines and C5a in lung injury after hindlimb ischemia-reperfusion

    DEFF Research Database (Denmark)

    Bless, N M; Warner, R L; Padgaonkar, V A

    1999-01-01

    We evaluated the roles of the C-X-C chemokines cytokine-induced neutrophil chemoattractant (CINC) and macrophage inflammatory protein-2 (MIP-2) as well as the complement activation product C5a in development of lung injury after hindlimb ischemia-reperfusion in rats. During reperfusion, CD11b...... and CD18, but not CD11a, were upregulated on neutrophils [bronchoalveolar lavage (BAL) and blood] and lung macrophages. BAL levels of CINC and MIP-2 were increased during the ischemic and reperfusion periods. Treatment with either anti-CINC or anti-MIP-2 IgG significantly reduced lung vascular......, 58, and 23%, respectively (P MIP-2 as well as the complement activation product C5a are required for lung neutrophil recruitment and full induction of lung injury after hindlimb ischemia-reperfusion in rats....

  5. Effects of micro-water on decomposition of the environment-friendly insulating medium C5F10O

    OpenAIRE

    Song Xiao; Yi Li; Xiaoxing Zhang; Shuangshuang Tian; Zaitao Deng; Ju Tang

    2017-01-01

    SF6 is widely used in all kinds of high-voltage electrical equipment because of its excellent insulation and arc-extinguishing performance. However, this compound leads to serious greenhouse effect, which harms the environment. Many research institutions are now actively in search of SF6 alternative gas. C5F10O has attracted much attention as an alternative gas with low global warming potential (GWP) and excellent dielectric strength. In this paper, we analyzed the possible decomposition path...

  6. The reaction of C5N− with acetylene as a possible intermediate step to produce large anions in Titan's ionosphere

    Czech Academy of Sciences Publication Activity Database

    Lindén, F.; Žabka, Ján; Polášek, Miroslav; Zymak, Illia; Geppert, W. D.

    2018-01-01

    Roč. 20, č. 8 (2018), s. 5377-5388 ISSN 1463-9076 R&D Projects: GA MŠk(CZ) LD14024; GA ČR(CZ) GA17-14200S Grant - others:COST(XE) CM1401 Institutional support: RVO:61388955 Keywords : investigation * C5N- * Titan Subject RIV: CF - Physical ; Theoretical Chemistry OBOR OECD: Physical chemistry Impact factor: 4.123, year: 2016

  7. Targeted Delivery of Neutralizing Anti-C5 Antibody to Renal Endothelium Prevents Complement-Dependent Tissue Damage

    Directory of Open Access Journals (Sweden)

    Paolo Durigutto

    2017-09-01

    Full Text Available Complement activation is largely implicated in the pathogenesis of several clinical conditions and its therapeutic neutralization has proven effective in preventing tissue and organ damage. A problem that still needs to be solved in the therapeutic control of complement-mediated diseases is how to avoid side effects associated with chronic neutralization of the complement system, in particular, the increased risk of infections. We addressed this issue developing a strategy based on the preferential delivery of a C5 complement inhibitor to the organ involved in the pathologic process. To this end, we generated Ergidina, a neutralizing recombinant anti-C5 human antibody coupled with a cyclic-RGD peptide, with a distinctive homing property for ischemic endothelial cells and effective in controlling tissue damage in a rat model of renal ischemia/reperfusion injury (IRI. As a result of its preferential localization on renal endothelium, the molecule induced complete inhibition of complement activation at tissue level, and local protection from complement-mediated tissue damage without affecting circulating C5. The ex vivo binding of Ergidina to surgically removed kidney exposed to cold ischemia supports its therapeutic use to prevent posttransplant IRI leading to delay of graft function. Moreover, the finding that the ex vivo binding of Ergidina was not restricted to the kidney, but was also seen on ischemic heart, suggests that this RGD-targeted anti-C5 antibody may represent a useful tool to treat organs prior to transplantation. Based on this evidence, we propose preliminary data showing that Ergidina is a novel targeted drug to prevent complement activation on the endothelium of ischemic kidney.

  8. Treatment with the C5a receptor antagonist ADC-1004 reduces myocardial infarction in a porcine ischemia-reperfusion model

    Directory of Open Access Journals (Sweden)

    Arheden Håkan

    2010-09-01

    Full Text Available Abstract Background Polymorphonuclear neutrophils, stimulated by the activated complement factor C5a, have been implicated in cardiac ischemia/reperfusion injury. ADC-1004 is a competitive C5a receptor antagonist that has been shown to inhibit complement related neutrophil activation. ADC-1004 shields the neutrophils from C5a activation before they enter the reperfused area, which could be a mechanistic advantage compared to previous C5a directed reperfusion therapies. We investigated if treatment with ADC-1004, according to a clinically applicable protocol, would reduce infarct size and microvascular obstruction in a large animal myocardial infarct model. Methods In anesthetized pigs (42-53 kg, a percutaneous coronary intervention balloon was inflated in the left anterior descending artery for 40 minutes, followed by 4 hours of reperfusion. Twenty minutes after balloon inflation the pigs were randomized to an intravenous bolus administration of ADC-1004 (175 mg, n = 8 or saline (9 mg/ml, n = 8. Area at risk (AAR was evaluated by ex vivo SPECT. Infarct size and microvascular obstruction were evaluated by ex vivo MRI. The observers were blinded to the treatment at randomization and analysis. Results ADC-1004 treatment reduced infarct size by 21% (ADC-1004: 58.3 ± 3.4 vs control: 74.1 ± 2.9%AAR, p = 0.007. Microvascular obstruction was similar between the groups (ADC-1004: 2.2 ± 1.2 vs control: 5.3 ± 2.5%AAR, p = 0.23. The mean plasma concentration of ADC-1004 was 83 ± 8 nM at sacrifice. There were no significant differences between the groups with respect to heart rate, mean arterial pressure, cardiac output and blood-gas data. Conclusions ADC-1004 treatment reduces myocardial ischemia-reperfusion injury and represents a novel treatment strategy of myocardial infarct with potential clinical applicability.

  9. Deep extractive and oxidative desulfurization of dibenzothiophene with C5H9NO·SnCl2 coordinated ionic liquid.

    Science.gov (United States)

    Li, Fa-tang; Kou, Cheng-guang; Sun, Zhi-min; Hao, Ying-juan; Liu, Rui-hong; Zhao, Di-shun

    2012-02-29

    A new C5H9NO·SnCl2 coordinated ionic liquid (IL) was prepared by reacting N-methyl-pyrrolidone with anhydrous SnCl2. Desulfurization of dibenzothiophene (DBT) via extraction and oxidation with C5H9NO·SnCl2 IL as extractant, H2O2 and equal mol of CH3COOH as oxidants was investigated. The Nernst partition coefficients k(N) of C5H9NO·SnCl2 IL for the DBT in n-octane was above 5.0, showing its excellent extraction ability. During the oxidative desulfurization process, the optimal molar ratio of H2O2/DBT was six. Sulfur removal of DBT in n-octane was 94.8% in 30 min at 30 °C under the conditions of H2O2/DBT molar ratio of six and V (IL):V (oil)=1:3. Moreover, the sulfur removal increased with increasing temperature because of the high reaction rate constant, low viscosity, and high solubility of dibenzothiophene-sulfone in the IL. The kinetics of oxidative desulfurization of DBT was also investigated, and the apparent activation energy was found to be 32.5 kJ/mol. The IL could be recycled six times without a significant decrease in activity. Copyright © 2011 Elsevier B.V. All rights reserved.

  10. Relationship of Circulating C5a and Complement Factor H Levels With Disease Control in Pregnant Women With Asthma.

    Science.gov (United States)

    Bohács, Anikó; Bikov, András; Ivancsó, István; Czaller, Ibolya; Böcskei, Renáta; Müller, Veronika; Rigó, János; Losonczy, György; Tamási, Lilla

    2016-04-01

    Asthma often complicates pregnancy and represents a risk of serious pregnancy complications. The complement system contributes to asthma pathogenesis and is up-regulated in healthy gestation as well. The anaphylatoxin C5a has a major pro-inflammatory role, and the complement factor H is a main soluble regulator protein both in asthma and during pregnancy; however, peripheral levels of these complement factors and their relationship to disease control have not yet been evaluated in pregnant subjects with asthma. The present study aimed to investigate circulating C5a and complement factor H levels in asthma (non-pregnant subjects with asthma; n = 19) and in pregnancy with asthma (pregnant subjects with asthma; n = 22), compared with healthy non-pregnant (n = 21) and healthy pregnant women (n = 13) and to test their relationship to clinical parameters of asthma (lung function, airway inflammation, and symptoms). Circulating C5a levels were higher in the pregnant asthma subject group compared with the healthy non-pregnant, healthy pregnant, and non-pregnant asthma groups: median 2.629 (interquartile range [IQR] 2.257-3.052) ng/mL versus 1.84 (IQR 1.576-2.563), 1.783 (IQR 0.6064-2.786), and 2.024 (IQR 1.232-2.615) ng/mL, respectively (P = .02 in all cases). C5a correlated negatively with FEV1 (r = -0.44, P = .039) and FVC values (r = -0.64, P = .001) in the pregnant asthma group and positively with fraction of exhaled nitric oxide levels in the non-pregnant asthma group (n = 12, r = 0.78, P = .004). Complement factor H levels were elevated in both the healthy pregnant and pregnant asthma subject groups compared with the healthy non-pregnant group (median 1,082 [IQR 734.9-1,224] and 910.7 [IQR 614.5-1076] μg/mL vs. 559.7 [IQR 388.7-783.1] μg/mL, P = .002 and P = .004, respectively) but not in the pregnant asthma group compared with the non-pregnant asthma group (median 687.4 [IQR 441.6-947.6] μg/mL, P = .10). Asthma during pregnancy increases the circulating level of

  11. Experimental and molecular dynamics simulation study of the sublimation and vaporization energetics of iron metalocenes. crystal structures of Fe(eta5-C5H4CH3)2 and Fe[(eta5-(C5H5)(eta5-C5H4CHO)].

    Science.gov (United States)

    Lousada, Claudio M; Pinto, Susana S; Lopes, José N Canongia; da Piedade, M Fatima Minas; Diogo, Hermínio P; da Piedade, Manuel E Minas

    2008-04-03

    The standard molar enthalpies of sublimation of ferrocene, 1,1'-dimethylferrocene, decamethylferrocene, ferrocenecarboxaldehyde and alpha-methylferrocenemethanol, and the enthalpy of vaporization of N,N-dimethyl(aminomethyl)ferrocene, at 298.15 K, were determined by Calvet-drop microcalorimetry and/or the Knudsen effusion method. The obtained values were used to assess and refine our previously developed force field for metallocenes. The modified force field was able to reproduce the deltasubHdegreesm and deltavapHdegreesm values of the test-set with an accuracy better than 5 kJ.mol-1, except for decamethylferrocene, in which case the deviation between the calculated and experimental deltasubHdegreesm values was 16.1 kJ.mol-1. The origin of the larger error found in the prediction of the sublimation energetics of decamethylferrocene, and which was also observed in the estimation of structural properties (e.g., density and unit cell dimensions), is discussed. Finally, the crystal structures of Fe(eta5-C5H4CH3)2 and Fe[(eta5-(C5H5)(eta5-C5H4CHO)] at 293 and 150 K, respectively, are reported.

  12. Wave Star C5

    DEFF Research Database (Denmark)

    Kramer, Morten; Kristensen, Tom Sten

    Design pile loads in this document are based on the Morison equation. In Chapter 3 and 4 the background for the design loads provided in Chapter 5 are given. In the remaining chapters from Chapter 6 and onward discussions and explanations of the results are given. A historical list of activities ...

  13. Differential contribution of complement receptor C5aR in myeloid and non-myeloid cells in chronic ethanol-induced liver injury in mice.

    Science.gov (United States)

    McCullough, Rebecca L; McMullen, Megan R; Das, Dola; Roychowdhury, Sanjoy; Strainic, Michael G; Medof, M Edward; Nagy, Laura E

    2016-07-01

    Complement is implicated in the development of alcoholic liver disease. C3 and C5 contribute to ethanol-induced liver injury; however, the role of C5a receptor (C5aR) on myeloid and non-myeloid cells to progression of injury is not known. C57BL/6 (WT), global C5aR-/-, myeloid-specific C5aR-/-, and non-myeloid-specific C5aR-/- mice were fed a Lieber-DeCarli diet (32%kcal EtOH) for 25 days. Cultured hepatocytes were challenged with ethanol, TNFα, and C5a. Chronic ethanol feeding increased expression of pro-inflammatory mediators in livers of WT mice; this response was completely blunted in C5aR-/- mice. However, C5aR-/- mice were not protected from other measures of hepatocellular damage, including ethanol-induced increases in hepatic triglycerides, plasma alanine aminotransferase and hepatocyte apoptosis. CYP2E1 and 4-hydroxynonenal protein adducts were induced in WT and C5aR-/- mice. Myeloid-specific C5aR-/- mice were protected from ethanol-induced increases in hepatic TNFα, whereas non-myeloid-specific C5aR-/- displayed increased hepatocyte apoptosis and inflammation after chronic ethanol feeding. In cultured hepatocytes, cytotoxicity induced by challenge with ethanol and TNFα was completely eliminated by treatment with C5a in cells from WT, but not C5aR-/- mice. Further, treatment with C5a enhanced activation of pro-survival signal AKT in hepatocytes challenged with ethanol and TNFα. Taken together, these data reveal a differential role for C5aR during ethanol-induced liver inflammation and injury, with C5aR on myeloid cells contributing to ethanol-induced inflammatory cytokine expression, while non-myeloid C5aR protects hepatocytes from death after chronic ethanol feeding. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Synthesis and chemistry of the open-cage cobaltaheteroborane cluster [{(η(5)-C5Me5)Co}2B2H2Se2]: a combined experimental and theoretical study.

    Science.gov (United States)

    Barik, Subrat Kumar; Dorcet, Vincent; Roisnel, Thierry; Halet, Jean-François; Ghosh, Sundargopal

    2015-08-28

    Reaction of [(η(5)-C5Me5)CoCl]2 with a two-fold excess of [LiBH4·thf] followed by heating with an excess of Se powder produces the dicobaltaselenaborane species [{(η(5)-C5Me5)Co}2B2H2Se2], , in good yield. The geometry of resembles a nido pentagonal [Co2B2Se2] bipyramid with a missing equatorial vertex. It can alternatively be seen as an open cage triple-decker cluster. Isolation of permits its reaction with [Fe2(CO)9] to give heterometallic diselenametallaborane [{(η(5)-C5Me5)Co}Fe(CO)3B2H2Se2], . The geometry of is similar to that of with one of the [(η(5)-C5Me5)Co] groups replaced by the isolobal, two-electron fragment [Fe(CO)3]. Both new compounds have been characterized by mass spectrometry, and by (1)H, (11)B and (13)C NMR spectroscopy. The structural architectures have been unequivocally established by crystallographic analysis. In addition, density functional theory calculations were performed to investigate the bonding and electronic properties. The large HOMO-LUMO gaps computed for both clusters are consistent with their thermodynamic stability. Natural bond order calculations predict the absence of metal-metal bonding interaction.

  15. Characterisation of 5-HT3C, 5-HT3D and 5-HT3E receptor subunits: evolution, distribution and function.

    Science.gov (United States)

    Holbrook, Joanna D; Gill, Catherine H; Zebda, Noureddine; Spencer, Jon P; Leyland, Rebecca; Rance, Kim H; Trinh, Han; Balmer, Gemma; Kelly, Fiona M; Yusaf, Shahnaz P; Courtenay, Nicola; Luck, Jane; Rhodes, Andrew; Modha, Sundip; Moore, Stephen E; Sanger, Gareth J; Gunthorpe, Martin J

    2009-01-01

    The 5-HT(3) receptor is a member of the 'Cys-loop' family of ligand-gated ion channels that mediate fast excitatory and inhibitory transmission in the nervous system. Current evidence points towards native 5-HT(3) receptors originating from homomeric assemblies of 5-HT(3A) or heteromeric assembly of 5-HT(3A) and 5-HT(3B). Novel genes encoding 5-HT(3C), 5-HT(3D), and 5-HT(3E) have recently been described but the functional importance of these proteins is unknown. In the present study, in silico analysis (confirmed by partial cloning) indicated that 5-HT(3C), 5-HT(3D), and 5-HT(3E) are not human-specific as previously reported: they are conserved in multiple mammalian species but are absent in rodents. Expression profiles of the novel human genes indicated high levels in the gastrointestinal tract but also in the brain, Dorsal Root Ganglion (DRG) and other tissues. Following the demonstration that these subunits are expressed at the cell membrane, the functional properties of the recombinant human subunits were investigated using patch clamp electrophysiology. 5-HT(3C), 5-HT(3D), and 5-HT(3E) were all non-functional when expressed alone. Co-transfection studies to determine potential novel heteromeric receptor interactions with 5-HT(3A) demonstrated that the expression or function of the receptor was modified by 5-HT(3C) and 5-HT(3E), but not 5-HT(3D). The lack of distinct effects on current rectification, kinetics or pharmacology of 5-HT(3A) receptors does not however provide unequivocal evidence to support a direct contribution of 5-HT(3C) or 5-HT(3E) to the lining of the ion channel pore of novel heteromeric receptors. The functional and pharmacological contributions of these novel subunits to human biology and diseases such as irritable bowel syndrome for which 5-HT(3) receptor antagonists have major clinical usage, therefore remains to be fully determined.

  16. Serum cholinesterase polymorphism (CHE1 and CHE2 loci) among several Indian groups from Amazon region of Brazil, and segregation of the C5 variant in families.

    Science.gov (United States)

    Guerreiro, J F; Santos, S E; Aguiar, G F

    1989-04-01

    Eight Indian tribes from the Amazon region of Brazil (Araweté, Arara, Yamamadi, Kararaô, Karitiana, Waiampi, Surui and Cinta Larga) were studied for the distribution of the atypical and C5 variants of serum cholinesterase. None of them presented the CHE1*A allele, but the C5 variant was found in the Araweté (20.4%), Kararaô (15.6%), Karitiana (50.5%), Surui (12.3%) and Cinta Larga (19.6%) tribes. The frequency of the C5+ phenotype in the Karitiana is the highest reported thus far in human populations. Segregation studies considering the C5 variant were made among the Karitiana, and also among the Urubu-Kaapor and Munduruku tribes previously studied by Guerreiro et al [1987a, 1987b]. Most of the data were in agreement with the genetic hypothesis, but they also revealed a significant lack of the C5+ phenotype in offspring from C5+ X C5+ matings, as well as the occurrence of two C5+ children from C5- X C5- unions, in the Urubu-Kaapor tribe.

  17. Chemical conjugation of 2-hexadecynoic acid to C5-curcumin enhances its antibacterial activity against multi-drug resistant bacteria.

    Science.gov (United States)

    Sanabria-Ríos, David J; Rivera-Torres, Yaritza; Rosario, Joshua; Gutierrez, Ricardo; Torres-García, Yeireliz; Montano, Nashbly; Ortíz-Soto, Gabriela; Ríos-Olivares, Eddy; Rodríguez, José W; Carballeira, Néstor M

    2015-11-15

    The first total synthesis of a C5-curcumin-2-hexadecynoic acid (C5-Curc-2-HDA, 6) conjugate was successfully performed. Through a three-step synthetic route, conjugate 6 was obtained in 13% overall yield and tested for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) strains. Our results revealed that 6 was active against eight MRSA strains at MICs that range between 31.3 and 62.5 μg/mL. It was found that the presence of 2-hexadecynoic acid (2-HDA, 4) in conjugate 6 increased 4-8-fold its antibacterial activity against MRSA strains supporting our hypothesis that the chemical connection of 4 to C5-curcumin (2) increases the antibacterial activity of 2 against Gram-positive bacteria. Combinational index (CIn) values that range between 1.6 and 2.3 were obtained when eight MRSA strains were treated with an equimolar mixture of 2 and 4. These results demonstrated that an antagonistic effect is taking place. Finally, it was investigated whether conjugate 6 can affect the replication process of S. aureus, since this compound inhibited the supercoiling activity of the S. aureus DNA gyrase at minimum inhibitory concentrations (MIC) of 250 μg/mL (IC50=100.2±13.9 μg/mL). Moreover, it was observed that the presence of 4 in conjugate 6 improves the anti-topoisomerase activity of 2 towards S. aureus DNA gyrase, which is in agreement with results obtained from antibacterial susceptibility tests involving MRSA strains. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Intrathecal Spread of Injectate Following an Ultrasound-Guided Selective C5 Nerve Root Injection in a Human Cadaver Model.

    Science.gov (United States)

    Falyar, Christian R; Abercrombie, Caroline; Becker, Robert; Biddle, Chuck

    2016-04-01

    Ultrasound-guided selective C5 nerve root blocks have been described in several case reports as a safe and effective means to anesthetize the distal clavicle while maintaining innervation of the upper extremity and preserving diaphragmatic function. In this study, cadavers were injected with 5 mL of 0.5% methylene blue dye under ultrasound guidance to investigate possible proximal and distal spread of injectate along the brachial plexus, if any. Following the injections, the specimens were dissected and examined to determine the distribution of dye and the structures affected. One injection revealed dye extended proximally into the epidural space, which penetrated the dura mater and was present on the spinal cord and brainstem. Dye was noted distally to the divisions in 3 injections. The anterior scalene muscle and phrenic nerve were stained in all 4 injections. It appears unlikely that local anesthetic spread is limited to the nerve root following an ultrasound-guided selective C5 nerve root injection. Under certain conditions, intrathecal spread also appears possible, which has major patient safety implications. Additional safety measures, such as injection pressure monitoring, should be incorporated into this block, or approaches that are more distal should be considered for the acute pain management of distal clavicle fractures.

  19. Effect of short-range ordering on the electrical conductivity and superconducting properties of Nb6C5 single crystals

    International Nuclear Information System (INIS)

    Utkina, T.G.

    1995-01-01

    Niobium carbide, NbC x , belongs to the family of so called interstital phases. Metal atoms form a face-centered cubic lattice, whose octahedral interstices are occupied by carbon atoms. The fraction (1 - x) of interstices remain vacant, and this determines the nonstoichiometry of these phases: most of them are characterized by a wide homogeneity range, 0.70 m ≅ 3308 - 3886 K). In contrast, the metalloid atoms exhibit high mobility at relatively low temperatures. For compositions close to Nb 6 C 5 (0.81 O-D ≅ 1300 K. The presence of vacancies in the carbon sublattice considerably affects the physical properties of carbides, which depend not only on total vacancy concentration but also on their distribution, i.e., on the degree of ordering (both short-range and long-range order) in the metalloid sublattice. The purpose of this work is to study the effects of such ordering on the superconducting properties of Nb 6 C 5 single crystals

  20. Activation of SO2 with [(η(5) -C5 Me5 )2 Ln(THF)2 ] (Ln=Eu, Yb) leading to dithionite and sulfinate complexes.

    Science.gov (United States)

    Klementyeva, Svetlana V; Gamer, Michael T; Schmidt, Anna-Corina; Meyer, Karsten; Konchenko, Sergey N; Roesky, Peter W

    2014-10-13

    The reaction of decamethylytterbocene [(η(5) -C5 Me5 )2 Yb(THF)2 ] with SO2 at low temperature gave two new compounds, namely, the Yb(III) dithionite/sulfinate complex [{(η(5) -C5 Me5 )2 Yb(μ3 ,1κ(2) O(1,3) ,2κ(3) O(2,2',4) -S2 O4 )}2 {(η(5) -C5 Me5 )Yb(μ,1κO,2κO'-C5 Me5 SO2 )}2 ] (1) and the Yb(III) dithionite complex [{(η(5) -C5 Me5 )2 Yb}2 (μ,1κ(2) O(1,3) ,2κ(2) O(2,4) -S2 O4 )] (2). After extraction of 1, the mixture was heated to give the dinuclear tetrasulfinate complex [{(η(5) -C5 Me5 )Yb}2 (μ,κO,κO'-C5 Me5 SO2 )4 ] (3 a). In contrast, from the reaction of [(η(5) -C5 Me5 )2 Eu(THF)2 ] with SO2 only the tetrasulfinate complex [{(η(5) -C5 Me5 )Eu}2 (μ,κO,κO'-C5 Me5 SO2 )4 ] (3 b) was isolated. Two major reaction pathways were observed: 1) reductive coupling of two SO2 molecules to form the dithionite anion S2 O4 (2-) ; and 2) nucleophilic attack of one metallocene C5 Me5 ligand on the sulfur atom of SO2 . The compounds presented are the first dithionite and sulfinate complexes of the f-elements. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Structural Necessity of Indole C5-O-Substitution of seco-Duocarmycin Analogs for Their Cytotoxic Activity

    Directory of Open Access Journals (Sweden)

    Taeyoung Choi

    2010-11-01

    Full Text Available A series of racemic indole C5-O-substituted seco-cyclopropylindole (seco-CI compounds 1-5 were prepared by coupling in the presence of EDCI of 1-(tert-butyloxycarbonyl-3-(chloromethylindoline (seg-A with 5-hydroxy-, 5-O-methylsulfonyl, 5-O-aminosulfonyl, 5-O-(N,N-dimethylaminosulfonyl- and 5-O-benzyl-1H-indole-2-carboxylic acid as seg-B. Compounds 1-5 were tested for cytotoxic activity against four human cancer cell lines (COLO 205, SK-MEL-2, A549, and JEG-3 using a MTT assay. Compounds 2 and 3 with small sized sulfonyl substituents like 5-O-methylsulfonyl and 5-O-aminosulfonyl exhibit a similar level of activity as doxorubicin against all cell lines tested.

  2. A Mathematical Model for Simultaneous Saccharification and Co-fermentation (SSCF) of C6 and C5 Sugars

    DEFF Research Database (Denmark)

    Morales Rodriguez, Ricardo; Gernaey, Krist; Meyer, Anne S.

    2011-01-01

    saccharification and co-fermentation (SSCF) of C6 and C5 sugars. The model is constructed by combining existing mathematical models for enzymatic hydrolysis and co-fermentation. An inhibition of ethanol on cellulose conversion is introduced in order to increase the reliability. The mathematical model for the SSCF...... is verified by comparing the model predictions with experimental data obtained from the ethanol production based on kraft paper mill sludge. When fitting the model to the data, only the yield coefficients for glucose and xylose metabolism were fine-tuned, which were found to be 0.43 g·g−1 (ethanol....../glucose) and 0.35 g·g−1 (ethanol/xylose) respectively. These promising validation results encourage further model application to evaluate different process configurations for lignocellulosic bioethanol technology....

  3. Structure of tris(trimethylsilylcyclopentadienyl)uranium(III), [(CH3)3SiC5H43U

    International Nuclear Information System (INIS)

    Brennan, J.; Andersen, R.A.; Zalkin, A.

    1986-02-01

    Crystals of [(CH 3 ) 3 SiC 5 H 4 ] 3 U are orthorhombic, Pbca, with a = 22.630(8), b = 29.177(10) and c = 8.428(3) A at 23 0 C. For Z = 8 the calculated density is 1.551 g/cm 3 . The structure was refined by full-matrix least-squares to a conventional R factor of 0.041 [2251 data, F 2 > 2 sigma(F 2 )]. The uranium atom is bonded to the three cyclopentadienyl rings in a pentahapto fashion and is in the plane of the ring centroids. The U to ring distances are 2.54, 2.47 and 2.51 A, and the average U-C distance is 2.78 +- 0.04 A. 7 refs., 1 fig., 3 tabs

  4. A PPy-B15C5 modified lanthanum (III electrode in acetonitrile and its thermodynamic application

    Directory of Open Access Journals (Sweden)

    Mohammad Hossein Arbab Zavar

    2017-02-01

    Full Text Available Polypyrrole modified electrode prepared by electropolymerization of pyrrole in the presence of a complexing ligand, benzo-15-crown-5 (B15C5, was prepared and investigated as a La3+-selective electrode in acetonitrile. The potentiometric response of the electrode was linear within the La3+ concentration range 1 × 10−4 to 1 × 10−1 M with a Nernstian slope of 19.5 mV decade−1 in AN. The electrode was applied to study the complexation of the lanthanum (III ion in acetonitrile with other basic solvent molecules (D such as dimethyl sulfoxide, N,N-dimethylformamide, propylene carbonate, N,N,Diethylaniline and methanol. The successive complex formation constant (βi and Gibbs energies of transfer (ΔGtr of La3+ in AN in relation to such D were obtained.

  5. Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

    DEFF Research Database (Denmark)

    Biswas, Rajib; Uellendahl, Hinrich; Ahring, Birgitte Kiær

    2013-01-01

    Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C6 and C5 sugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation...... products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification...... using Scheffersomyces (Pichia) stipitis CBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Yp/s) ranged from 88-100% and 0.33-0.41 ± 0.02 g/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185°C and 6 bar O2, composed...

  6. The new deterministic 3-D radiation transport code Multitrans: C5G7 MOX fuel assembly benchmark

    International Nuclear Information System (INIS)

    Kotiluoto, P.

    2003-01-01

    The novel deterministic three-dimensional radiation transport code MultiTrans is based on combination of the advanced tree multigrid technique and the simplified P3 (SP3) radiation transport approximation. In the tree multigrid technique, an automatic mesh refinement is performed on material surfaces. The tree multigrid is generated directly from stereo-lithography (STL) files exported by computer-aided design (CAD) systems, thus allowing an easy interface for construction and upgrading of the geometry. The deterministic MultiTrans code allows fast solution of complicated three-dimensional transport problems in detail, offering a new tool for nuclear applications in reactor physics. In order to determine the feasibility of a new code, computational benchmarks need to be carried out. In this work, MultiTrans code is tested for a seven-group three-dimensional MOX fuel assembly transport benchmark without spatial homogenization (NEA C5G7 MOX). (author)

  7. Kinetics of [14C-5] 8-methoxypsoralen uptake by UVA irradiated and non-irradiated rabbit eye tissues

    International Nuclear Information System (INIS)

    Malinin, G.I.; Glew, W.B.; Roberts, W.P.; Nigra, T.P.

    1981-01-01

    Total 8-methoxypsoralen (8-MOP) in intact and UVA irradiated rabbit eye tissues and its unaltered fraction in aqueous and eye lenses were determined over the 24 hours after i.v. injection of [ 14 C-5] and carrier 8-MOP at the concentration of 50 microCi and 5 mg/kg. No 8-MOP was detected at the end of 24 hours in intact and irradiated aqueous, vitreous and retina in contrast to one hour when the respective levels were congruent to 220, greater than 0 and congruent to 160 ng/g. Eye-plasma drug concentration ratios were less than 0.5 initially, but increased thereafter. While the average lens 8-MOP levels of congruent to 140 ng/g remained unchanged for 24 hours, no unaltered drug was detected beyond eight hours. Measurable amounts of label at the end of 24 hours also persisted in the cornea, iris, sclera and conjunctiva

  8. Summertime C1-C5 alkyl nitrates over Beijing, northern China: Spatial distribution, regional transport, and formation mechanisms

    Science.gov (United States)

    Sun, Jingjing; Li, Zeyuan; Xue, Likun; Wang, Tao; Wang, Xinfeng; Gao, Jian; Nie, Wei; Simpson, Isobel J.; Gao, Rui; Blake, Donald R.; Chai, Fahe; Wang, Wenxing

    2018-05-01

    Alkyl nitrates (RONO2) are an important class of nitrogen oxides reservoirs in the atmosphere and play a key role in tropospheric photochemistry. Despite the increasing concern for photochemical air pollution over China, the knowledge of characteristics and formation mechanisms of alkyl nitrates in this region is limited. We analyzed C1-C5 alkyl nitrates measured in Beijing at a polluted urban site in summer 2008 and at a downwind rural site in summers of both 2005 and 2008. Although the abundances of NOx and hydrocarbons were much lower at the rural site, the mixing ratios of RONO2 were comparable between both sites, emphasizing the regional nature of alkyl nitrate pollution. Regional transport of urban plumes governed the elevated RONO2 levels at the rural site. The concentrations of C1-C2 RONO2 were significantly higher at the rural site in 2008 compared to 2005 despite a decline in NOx and anthropogenic VOCs, mainly owing to enhanced contributions from biogenic VOCs. The photochemical formation regimes of RONO2 were evaluated by both a simplified sequential reaction model and a detailed master chemical mechanism box model. The observed C4-C5 RONO2 levels can be well explained by the photochemical degradation of n-butane and n-pentane, while the sources of C1-C3 RONO2 were rather complex. In addition to the C1-C3 alkanes, biogenic VOCs and reactive aromatics were also important precursors of methyl nitrate, and alkenes and long-chain alkanes contributed to the formation of C2-C3 RONO2. This study provides insights into the spatial distribution, inter-annual variation and photochemical formation mechanisms of alkyl nitrate pollution over the Beijing area.

  9. Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli

    Science.gov (United States)

    George, Kevin W.; Thompson, Mitchell G.; Kang, Aram; Baidoo, Edward; Wang, George; Chan, Leanne Jade G.; Adams, Paul D.; Petzold, Christopher J.; Keasling, Jay D.; Soon Lee, Taek

    2015-01-01

    Branched five carbon (C5) alcohols are attractive targets for microbial production due to their desirable fuel properties and importance as platform chemicals. In this study, we engineered a heterologous isoprenoid pathway in E. coli for the high-yield production of 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol, and 3-methyl-1-butanol, three C5 alcohols that serve as potential biofuels. We first constructed a pathway for 3-methyl-3-buten-1-ol, where metabolite profiling identified NudB, a promiscuous phosphatase, as a likely pathway bottleneck. We achieved a 60% increase in the yield of 3-methyl-3-buten-1-ol by engineering the Shine-Dalgarno sequence of nudB, which increased protein levels by 9-fold and reduced isopentenyl diphosphate (IPP) accumulation by 4-fold. To further optimize the pathway, we adjusted mevalonate kinase (MK) expression and investigated MK enzymes from alternative microbes such as Methanosarcina mazei. Next, we expressed a fusion protein of IPP isomerase and the phosphatase (Idi1~NudB) along with a reductase (NemA) to diversify production to 3-methyl-2-buten-1-ol and 3-methyl-1-butanol. Finally, we used an oleyl alcohol overlay to improve alcohol recovery, achieving final titers of 2.23 g/L of 3-methyl-3-buten-1-ol (~70% of pathway-dependent theoretical yield), 150 mg/L of 3-methyl-2-buten-1-ol, and 300 mg/L of 3-methyl-1-butanol. PMID:26052683

  10. Pro-C5, a marker of true type V collagen formation and fibrillation, correlates with portal hypertension in patients with alcoholic cirrhosis

    DEFF Research Database (Denmark)

    Leeming, Diana J; Veidal, Sanne S; Karsdal, Morten A

    2015-01-01

    Pro-C5 reflects liver hemodynamics, liver function, disease stage and clinically significant portal hypertension (PH). A multimarker model in combination with clinical scores predicted HVPG and separated clinical relevant HVPG thresholds. Plasma Pro-C5 may be suitable for the noninvasive evaluation...

  11. Structural and mutational characterization of the catalytic A-module of the mannuronan C-5-epimerase AlgE4 from Azotobacter vinelandii

    NARCIS (Netherlands)

    Rozeboom, Henriette J.; Bjerkan, Tonje M.; Kalk, Kor H.; Ertesvag, Helga; Holtan, Synnove; Aachmann, Finn L.; Valla, Svein; Dijkstra, Bauke W.; Ertesvåg, Helga; Holtan, Synnøve

    2008-01-01

    Alginate is a family of linear copolymers of (1 -> 4)-linked beta-D-mannuronic acid and its C-5 epimer alpha-L-guluronic acid. The polymer is first produced as polymannuronic acid and the guluronic acid residues are then introduced at the polymer level by mannuronan C-5-epimerases. The structure of

  12. Dual Inhibition of AChE and BChE with the C-5 Substituted Derivative of Meldrum’s Acid: Synthesis, Structure Elucidation, and Molecular Docking Studies

    Directory of Open Access Journals (Sweden)

    Haroon Mehfooz

    2017-07-01

    Full Text Available Alzheimer’s disease (AD lies in the category of those diseases which are still posing challenges to medicinal chemists, and the search for super-effective drugs for the treatment of AD is a work in progress. The inhibition of cholinesterase is considered a viable strategy to enhance the level of acetylcholine in the brain. The C-5 substituted derivative of Meldrum’s acid was synthesized and screened against acetylcholinesterase (AChE and butyrylcholinesterase (BChE enzyme inhibition activity. The simple and unique structure of synthesized derivative 3 was found to be good for the dual inhibition of both enzymes (AChE and BChE. 2,2-Dimethyl-5-(([2-(trifluoromethyl phenyl]aminomethylidene-1,3-dioxane-4,6-dione (3 showed significant inhibition against AChE, with an IC50 value of 1.13 ± 0.03 µ M (Standard Neostigmine 22.2 ± 3.2 µM, and moderate inhibition against BChE, with an IC50 value of 2.12 ± 1.22 µM (Standard Neostigmine 49.6 ± 6.11 µM. The structural insights reveal that compound 3 possesses intriguing reactive groups, which can potentially evoke the non-covalent interactions and possibly assist by binding in the active site of the target protein. Docking simulations revealed that the compound 3 showed binding inside the active site gorges of both AChE and BChE. An excellent agreement was obtained, as the best docked poses showed important binding features mostly based on interactions due to oxygen atoms and the aromatic moieties of the compound. The docking computations coupled with the experimental findings ascertained that the compound 3 can serve as a scaffold for the dual inhibitors of the human acetylcholine esterases.

  13. Construction in space - Toward a fresh definition of the man/machine relation

    Science.gov (United States)

    Watters, H. H.; Stokes, J. W.

    1979-01-01

    The EVA (extravehicular activity) project forming part of the space construction process is reviewed. The manual EVA constuction, demonstrated by the crew of Skylab 3 by assembling a modest space structure in the form of the twin-pole sunshade, is considered, indicating that the experiment dispelled many doubts about man's ability to execute routine and contingency EVA operations. Tests demonstrating the feasibility of remote teleoperator rendezvous, station keeping, and docking operations, using hand controllers for direct input and television for feedback, are noted. Future plans for designing space construction machines are mentioned.

  14. The development of KNGR control room man-machine interface design

    International Nuclear Information System (INIS)

    Sung-Jae Cho; Yeong-Cheol Shin

    2000-01-01

    KNGR MMI design has been developed for the last 7 years as a part of Korea Next Generation Reactor (KNGR) design development. The KNGR control room has the common features of advanced control room such as large display panel, redundant compact workstations, soft control, and computerized procedure system. A conventional type safety console is provided as a backup when operation at the workstations is impossible. The strong points of an advanced control room are based on the powerful information processing and flexible graphic presentation capability of computer technology. On the other hand, workstation based design has a weak point that the amount of information to be presented in one VDU is limited. This can cause navigational overload and inconsistent interfaces and provide chances for performance errors/failures, if not designed carefully. From this background, the regulators require licensees to follow strict top-down human factor engineering design process. Analysis of operating experiences and iterative evaluations are used to address the potential problems of the KNGR advanced control room MMI design. But, further study is necessary in design area like CPS design, where experiences or design guidance is insufficient. Further study topics for KNGR advanced control room MMI design development are discussed briefly in this paper. (author)

  15. Xenon bubble chamber image processing on the POISK installation in the man-machine dialog regime

    International Nuclear Information System (INIS)

    Avdeev, N.F.; Barylov, V.G.; Volkov, G.A.; Demidov, V.S.; Kalinina, G.V.; Kobzarev, K.K.; Sokolov, M.M.

    1980-01-01

    Procedure for image processing of xenon bubble chamber at POISK facilities operating in the dialog regime with the BESM-4 and BESM-6 computers is stated. Primary functions of the BESM-4 computer are step-by-step observations for the measurement process and control for experimental data transmitted by the communication line. The mathematical processing of events or massives of events is accomplished by the head BESM-6 computer. To realize the dialog regime of the facility operation with the BESM-4 and BESM-6 computers used are four VT-430 displays. This permits to exchange massives of alphabetic-numeric data of considerable volume with the computers. Directives and commands used with operators of the facilities during the work at the display keyboard are given [ru

  16. Man-machine-communication. Operation and supervision of complicated systems in a modern cockpit

    International Nuclear Information System (INIS)

    Schmitt, B.

    1985-01-01

    Redundance is vital in modern aircraft. For some systems a fourfold redundance is given. In the cockpit of the Airbus, colour screens are being used to display information needed by the pilots. Potentials of such a display are discussed

  17. Simulation model for man-machine systems in nuclear power plants. Vol. 3

    Energy Technology Data Exchange (ETDEWEB)

    Falk, A; Steinhagen, G U

    1981-01-01

    The present volume three tries to present working methods which are used for the evaluation of findings regarding the world of working described and compiled in volume 1 and 2. It is to quantify the found or invented data from the psychology of work more than other publications of work science have done. This combination of quantifying concepts and utilizing and assessing them by the help of EDP is new. Those theories on activation and communication are dealt with the development of which forms part of the tasks defined during and for this research project. Chapter six includes the theory of the evaluation factor 'working quality'. At first understood as a subjective psychological concept 'working quality' is considered a result starting from which links to other concepts of technology-orientated risk studies are to be made.

  18. Simulation model for man-machine systems in nuclear power plants. Vol. 3. Mathematical models

    Energy Technology Data Exchange (ETDEWEB)

    Falk, A; Steinhagen, G U

    1981-08-01

    The present volume three tries to present working methods which are used for the evaluation of findings regarding the world of working described and compiled in volume 1 and 2. It is to quantify the found or invented data from the psychology of work more than other publications of work science have done. This combination of quantifying concepts and utilizing and assessing them by the help of EDP is new. Those theories on activation and communication are dealt with the development of which forms part of the tasks defined during and for this research project. Chapter six includes the theory of the evaluation factor 'working quality'. At first understood as a subjective psychological concept 'working quality' is considered a result starting from which links to other concepts of technology-orientated risk studies are to be made.

  19. Design Integration of Man-Machine Interface (MMI) Display Drawings and MMI Database

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yong Jun; Seo, Kwang Rak; Song, Jeong Woog; Kim, Dae Ho; Han, Jung A [KEPCO Engineering and Construction Co., Deajeon (Korea, Republic of)

    2016-10-15

    The conventional Main Control Room (MCR) was designed using hardwired controllers and analog indications mounted on control boards for control and acquisition of plant information. This is compared with advanced MCR design where Flat Panel Displays (FPDs) with soft controls and mimic displays are used. The advanced design needs MMI display drawings replacing the conventional control board layout drawings and component lists. The data is linked to related object of the MMI displays. Compilation of the data into the DB is generally done manually, which tends to introduce errors and discrepancies. Also, updating and managing is difficult due to a huge number of entries in the DB and the update must closely track the changes in the associated drawing. Therefore, automating the DB update whenever a related drawing is updated would be quite beneficial. An attempt is made to develop a new method to integrate the MMIS display drawing design and the DB management. This would significantly reduce the amount of errors and improve design quality. The design integration of the MMI Display drawing and MMI DB is explained briefly but concisely in this paper. The existing method involved individually and separately inputting design data for the MMI display drawings. This caused to the potential problem of data discrepancies and errors as well as the update time lag between related drawings and the DB. This led to development of an integration of design process which automates the design data input activity.

  20. Challenges to a Man-Machine Interface System in Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Yeon Sub [KHNP, Daejeon (Korea, Republic of)

    2009-10-15

    When the APR1400 project started nearly 20 years ago, advanced features such as intelligent alarms, computer-based procedures, and soft control technology were introduced. Although some of these technologies had been utilized in fossil plants, APR1400 opened a new horizon in MMIS for nuclear power plants. Currently, however, it appears that further challenges do not exist in the MMIS area. Engineers are simply busy in designing and constructing the next nuclear plant without exploiting the new features of MMIS. This paper explains newly emerging and feasible technology and suggests new ideas for MMIS compared with other industries.

  1. Simulation model for man-machine systems in nuclear power plants. Vol. 2

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, H J

    1981-01-01

    The social and organizational structure of power plants which has been growing for decades has known a variant since the first operation of UPPS which results from an obligation to industrial safety in the broadest sense. The intensity of the relations among people at work as well as of the welfare measures working people have grown. In part 1 of this volume, the author tries to present the elements of qualification from the psychological sector by the help of which both the demands made on people at work and their personal qualification are briefly outlined. Part two and three present the relations of working people within a group and formulate some premises and already known results of motivation research and solidarity of the working group with regard to UPP operation.

  2. Simulation model for man-machine systems in nuclear power plants. Vol. 4

    Energy Technology Data Exchange (ETDEWEB)

    Falk, A

    1981-01-01

    At first, volume 4 was to describe the EDP-bound simulation model in the final programme version. This problem was tackled as a development project, however, it could not be solved because of the difficult time schedule and the commissioning of the new computing centre at the Fachhochschule Flensburg. The assignment described in volume 3, chapter 2, already contains the optimization stages planned for 1981 but still uses a special programming language 'Prozess-FORTRAN' for older digital computers. That is why the development of a new version of the programming system in Standard-FORTRAN should be postponed. It should also include a more comprehensive practical data bank for nuclear power plants. The present short report as well as the following more comprehensive final report lay more emphasis on the presentation of a functional range of application of the simulation model.

  3. Fused man-machine classification schemes to enhance diagnosis of breast microcalcifications

    Science.gov (United States)

    Andreadis, Ioannis; Sevastianos, Chatzistergos; George, Spyrou; Konstantina, Nikita

    2017-11-01

    Computer aided diagnosis (CAD x ) approaches are developed towards the effective discrimination between benign and malignant clusters of microcalcifications. Different sources of information are exploited, such as features extracted from the image analysis of the region of interest, features related to the location of the cluster inside the breast, age of the patient and descriptors provided by the radiologists while performing their diagnostic task. A series of different CAD x schemes are implemented, each of which uses a different category of features and adopts a variety of machine learning algorithms and alternative image processing techniques. A novel framework is introduced where these independent diagnostic components are properly combined according to features critical to a radiologist in an attempt to identify the most appropriate CAD x schemes for the case under consideration. An open access database (Digital Database of Screening Mammography (DDSM)) has been elaborated to construct a large dataset with cases of varying subtlety, in order to ensure the development of schemes with high generalization ability, as well as extensive evaluation of their performance. The obtained results indicate that the proposed framework succeeds in improving the diagnostic procedure, as the achieved overall classification performance outperforms all the independent single diagnostic components, as well as the radiologists that assessed the same cases, in terms of accuracy, sensitivity, specificity and area under the curve following receiver operating characteristic analysis.

  4. Development of a procedure for man-machine systems diagnose. Pt. 1

    International Nuclear Information System (INIS)

    Bernhardt, U.; Hauke, G.; Hoyos, C.; Wenninger, G.

    1984-01-01

    The goal of the research project was the development of a behavior-oriented instrument with the help of which safety experts in organizations can obtain preventive information on problem areas which are critical to safety. The evaluation of already-existing research results led to the construction of the ''Safety Diagnosis Questionnaire (SDQ)'', which was developed in close cooperation with organizational safety experts and was tested in various organizations. Part 1 of this report contains a description of the work steps involved in the project: determination of the elements of a man-environment system which are relevant to safety and which must be included in a diagnostic process; the formulation of ''safety elements'' as questions and the formal development of the SDQ; checking safety elements by means of questioning experts; testing the revised questionnaire in different organizations and making a final revision; evaluation of the gathered data. Part 2 of the report contains a description of measures intended to influence behavior so that it is conducive to safety. Interpretation aids for the practical application of the SDQ are introduced. The results of the first attempt to verify this concept for the development of measures are briefly described. (orig./GL) [de

  5. Simulation model for man-machine systems in nuclear power plants. Vol. 2

    International Nuclear Information System (INIS)

    Jensen, H.J.

    1981-01-01

    The social and organizational structure of power plants which has been growing for decades has known a variant since the first operation of upps which results from an obligation to industrial safety in the broadest sense. The intensity of the relations among people at work as well as of the welfare measures working people have grown. In part 1 of this volume, the author tries to present the elements of qualification from the psychological sector by the help of which both the demands made on people at work and their personal qualification are briefly outlined. Part two and three present the relations of working people within a group and formulate some premises and already known results of motivation research and solidarity of the working group with regard to upp operation. (orig.) [de

  6. Simulation model for man-machine systems in nuclear power plants. Vol. 3

    International Nuclear Information System (INIS)

    Falk, A.; Steinhagen, G.U.

    1981-01-01

    The present volume three tries to present working methods which are used for the evaluation of findings regarding the world of working described and compiled in volume 1 and 2. It is to quantify the found or invented data from the psychology of work more than other publications of work science have done. This combination of quantifying concepts and utilizing and assessing them by the help of EDP is new. Those theories on activation and communication are dealt with the development of which forms part of the tasks defined during and for this research project. Chapter six includes the theory of the evaluation factor 'working quality'. At first understood as a subjective psychological concept 'working quality' is considered a result starting from which links to other concepts of technology-orientated risk studies are to be made. (orig./GL) [de

  7. Tools for man-machine interface development in accelerator control applications

    International Nuclear Information System (INIS)

    Kopylov, L.; Mikhev, M.; Trofimov, N.; Yurpalov, V.

    1994-01-01

    For the UNK Project a development of the Accelerator Control Applications is in the progress. These applications will use a specific Graphical User Interface for data presentation and accelerator parameter management. A number of tools have been developed based on the Motif Tool Kit. They contain a set of problem oriented screen templates and libraries. Using these tools, full scale prototype applications of the UNK Tune and Orbit measurement and correction were developed and are described, as examples. A subset of these allows the creation of the synoptic control screens from the Autocad pictures files and Oracle DB equipment descriptions. The basic concepts and a few application examples are presented. ((orig.))

  8. Progress in instrumentation and control including the man-machine interface

    International Nuclear Information System (INIS)

    Wahlstroem, B.; Pedersen, T.; Neboyan, V.

    1999-01-01

    The paper discusses benefits and difficulties associated with the use of new digital instrumentation and control (I and C) systems for nuclear applications. The rapid development of information technology has not been used to the same extent in the nuclear industry as in conventional industries. The most important reason for this under-representation is a de-facto moratorium for construction of new plants. In old nuclear power plants (NPPs) the new technology is used in modernisation projects and valuable experience has been obtained. The licensing of programmable systems presents new challenges especially for safety systems where a very high integrity is required. The paper discusses various aspects related to the use of the new systems for nuclear applications, it gives references to ongoing work of international organisations and to research that is seen as an effort to solve problems related to implementation of the new systems for nuclear applications. (author)

  9. A study on the human factors of nuclear power plants man-machine interface

    International Nuclear Information System (INIS)

    Shin, Hyun Kook; Lee, Jeong Woon; Lee, Yong Hee; Oh, In Suk; Park, Geun Ok

    1991-01-01

    Approaches of the MMIS design were analysed in this project. Both systems approach and experimental approach were studied. In addition, guidelines for the design of MMI are discussed. The methodology of experimental approach studied in this project can be useful for establishing experimental criteria on MMI design items. The framework of a computer code was developed by utilizing function-based task analysis and procedure information requirements. This can be further developed as a tool useful for the verification and validation of MMI designs. A test facility was designed in compliance with the purpose of experimental approach by considering specific conditions and factors inherent in nuclear power plants. Test facility can be used for conducting experiments which provide detailed human performance and characteristics data at the low level of systems approach. An experimental study was performed to obtain a range of illumination levels suitable for the operation in VDU-based control rooms. Existing design guidelines were tested by this experimental study. A conceptual design of MMIS was developed in accordance with the established MMIS design approach and was evaluated through the comparison to other advanced control room designs. (Author)

  10. Allocatin of functions in man-machine systems: a perspective and literature review

    International Nuclear Information System (INIS)

    Price, H.E.; Maisano, R.E.; Van Cott, H.P.

    1982-06-01

    This report reviews the literature relevant to allocation of functions and presents a procedure for the allocation process applicable to nuclear power plant control rooms. An historical perspective of man's relationship with technology is given as background. Methods and models that have been developed to aid the allocation process are then considered, followed by examples of real-world applications. The relationship of allocation of function to the system development process is outlined. The report then turns to the proposed procedure of the allocation process

  11. Man-machine analysis of translation and work tasks of Skylab films

    Science.gov (United States)

    Hosler, W. W.; Boelter, J. G.; Morrow, J. R., Jr.; Jackson, J. T.

    1979-01-01

    An objective approach to determine the concurrent validity of computer-graphic models is real time film analysis. This technique was illustrated through the procedures and results obtained in an evaluation of translation of Skylab mission astronauts. The quantitative analysis was facilitated by the use of an electronic film analyzer, minicomputer, and specifically supportive software. The uses of this technique for human factors research are: (1) validation of theoretical operator models; (2) biokinetic analysis; (3) objective data evaluation; (4) dynamic anthropometry; (5) empirical time-line analysis; and (6) consideration of human variability. Computer assisted techniques for interface design and evaluation have the potential for improving the capability for human factors engineering.

  12. Fused man-machine classification schemes to enhance diagnosis of breast microcalcifications

    International Nuclear Information System (INIS)

    Andreadis, Ioannis; Sevastianos, Chatzistergos; Konstantina, Nikita; George, Spyrou

    2017-01-01

    Computer aided diagnosis (CAD x ) approaches are developed towards the effective discrimination between benign and malignant clusters of microcalcifications. Different sources of information are exploited, such as features extracted from the image analysis of the region of interest, features related to the location of the cluster inside the breast, age of the patient and descriptors provided by the radiologists while performing their diagnostic task. A series of different CAD x schemes are implemented, each of which uses a different category of features and adopts a variety of machine learning algorithms and alternative image processing techniques. A novel framework is introduced where these independent diagnostic components are properly combined according to features critical to a radiologist in an attempt to identify the most appropriate CAD x schemes for the case under consideration. An open access database (Digital Database of Screening Mammography (DDSM)) has been elaborated to construct a large dataset with cases of varying subtlety, in order to ensure the development of schemes with high generalization ability, as well as extensive evaluation of their performance. The obtained results indicate that the proposed framework succeeds in improving the diagnostic procedure, as the achieved overall classification performance outperforms all the independent single diagnostic components, as well as the radiologists that assessed the same cases, in terms of accuracy, sensitivity, specificity and area under the curve following receiver operating characteristic analysis. (paper)

  13. Technical requirements of the man-machine interface during operation of nuclear power plants

    International Nuclear Information System (INIS)

    Cakir, A.

    1992-01-01

    There are rules governing the shaping of the work-place and work environment, selection of personnel, and training for control rooms and back-up shutdown centers. The procedure under these items is, in many points, fixed by KTA 3904. That set of rules is the basis for translating into practice the system-ergonomic way of thinking. (DG) [de

  14. Challenges to a Man-Machine Interface System in Nuclear Power Plants

    International Nuclear Information System (INIS)

    Jung, Yeon Sub

    2009-01-01

    When the APR1400 project started nearly 20 years ago, advanced features such as intelligent alarms, computer-based procedures, and soft control technology were introduced. Although some of these technologies had been utilized in fossil plants, APR1400 opened a new horizon in MMIS for nuclear power plants. Currently, however, it appears that further challenges do not exist in the MMIS area. Engineers are simply busy in designing and constructing the next nuclear plant without exploiting the new features of MMIS. This paper explains newly emerging and feasible technology and suggests new ideas for MMIS compared with other industries

  15. Allocatin of functions in man-machine systems: a perspective and literature review

    Energy Technology Data Exchange (ETDEWEB)

    Price, H. E.; Maisano, R. E.; Van Cott, H. P.

    1982-06-01

    This report reviews the literature relevant to allocation of functions and presents a procedure for the allocation process applicable to nuclear power plant control rooms. An historical perspective of man's relationship with technology is given as background. Methods and models that have been developed to aid the allocation process are then considered, followed by examples of real-world applications. The relationship of allocation of function to the system development process is outlined. The report then turns to the proposed procedure of the allocation process.

  16. Man-machine communication in Portuguese: a friendly library service system

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, H

    1982-01-01

    This paper enhances some of the main aspects of a system which converses in Portuguese to provide a library service covering the field of artificial intelligence. Its central feature is the use of logic as a single uniform language for knowledge and data representation, deductive information retrieval and linguistic analysis. The objective of designing this system was the development of a feasible method for consulting and creating data bases in natural Portuguese. The system is implemented in PROLOG, a programming language essentially identical in syntax and semantics to a subset of predicate calculus in clausal form. 32 references.

  17. Man-Machine Communication in Remote Manipulation: Task-Oriented Supervisory Command Language (TOSC).

    Science.gov (United States)

    1980-03-01

    2-32 WvNIPUa.ATY wWMj.IAYCOYOLLID 𔃾 -XNEMILPU . 310?!3us mI.I Sr 1lR ROBO ........... O4WIUVISYN CP"CRIAumaN OWW411 -femi y PACISS PSTISH ’uucomC...task description 3-15 and as a machine advisor for an apprentice. Thus, the PN are developed hierarchically, by the machine from the top down to the...96734 rs Defense Technical Information Center Dr. A. L. Slafkosky Cameron Station, Bldg. 5 Scientific Advisor Alexandria, VA 22314 (12 cys) Commandant

  18. Advanced man-machine interface systems and advanced information management systems programs

    International Nuclear Information System (INIS)

    Naser, J.; Gray, S.; Machiels, A.

    1997-01-01

    The Advanced Light Water Reactor (ALWR) Program started in the early 1980's. This work involves the development and NRC review of the ALWR Utility Requirements Documents, the development and design certification of ALWR designs, the analysis of the Early Site Permit process, and the First-of-a-Kind Engineering for two of the ALWR plant designs. ALWRs will embody modern proven technology. However, technologies expected to be used in these plants are changing very rapidly so that additional capabilities will become available that will be beneficial for future plants. To remain competitive on a life-cycle basis in the future, the ALWR must take advantage of the best and most modem technologies available. 1 ref

  19. Acquisition of Cooperative Small Unmanned Aerial Systems for Advancing Man Machine Interface Research

    Science.gov (United States)

    2016-08-24

    Airborne Cross Runway Departure/Landing Scenario Figure 7 Illustration of a participant looking at the 10Hz or 15Hz flickering visual stimulus in a...Figure 5 An illustration using a formation of UAVs for Direction of Arrival (DOA) mitigation of spoofing signals Spoofer Antenna array based on a...this project is to research and develop robust and jamming-resistant receivers including advanced interference mitigation algorithms and network

  20. Simulation technology - A key to effective man-machine integration for future combat rotorcraft systems

    Science.gov (United States)

    Kerr, Andrew W.

    1990-01-01

    The utilization of advanced simulation technology in the development of the non-real-time MANPRINT design tools in the Army/NASA Aircrew-Aircraft Integration (A3I) program is described. A description is then given of the Crew Station Research and Development Facilities, the primary tool for the application of MANPRINT principles. The purpose of the A3I program is to develop a rational, predictive methodology for helicopter cockpit system design that integrates human factors engineering with other principles at an early stage in the development process, avoiding the high cost of previous system design methods. Enabling technologies such as the MIDAS work station are examined, and the potential of low-cost parallel-processing systems is indicated.

  1. Optimization of hydrothermal pretreatment for co-utilization C-5 and C-6 sugars of cassava alcohol residue

    International Nuclear Information System (INIS)

    Lu, Huisheng; Lv, Chunliu; Zhang, Minhua; Liu, Shuangyan; Liu, Jiatao; Lian, Feng

    2017-01-01

    Highlights: • Cassava alcohol residue was first pretreated by hydrothermal reaction. • Hydrothermal pretreatment was optimized by RSM for co-utilization of C-5 and C-6 sugars. • The maximum xylose yield and the highest enzymatic digestibility were not obtained at the same conditions. • Optimum pretreatment conditions were at 193 °C, with 11.4% solids and for 51 min. • The optimal theoretical ethanol production was 69.5 mg/g raw materials by co-utilization of C-5 and C-6 sugars. - Abstract: Hydrothermal reaction was first applied to pretreat cassava alcohol residue for realizing the co-utilization of xylose and glucose to assume fermentation ethanol. This work focused on the influence of hydrothermal pretreatment conditions on ethanol production. Hydrothermal reaction was used to explore the maximum xylose and glucose yields, in respect to reaction temperature (120–240 °C), solid-liquid ratio (0.023–0.150) and reaction time (15–120 min). The results showed that the suitable conditions were at 180–200 °C, for 45–60 min and with 10–12.5% solids. In this range, the conjunct of xylose and glucose would reach the maximum, which can make full use of hemicellulose and cellulose in cassava alcohol residue. According to the results, respond surface methodology (RSM) based on Box-Behnken design was used to further optimize the three independent variables for the highest ethanol by co-utilization of xylose and glucose. RSM revealed that the effect of temperature on ethanol production was much more significant than the effect of reaction time and solid-liquid ratio, and the highest ethanol production was 70.6 mg/g that was close to the experiment value of 69.5 mg/g at 193 °C for 51 min and with 11.5% solids. Furthermore, the crystallinity and morphology of the untreated and pretreated cassava alcohol residue were investigated to assess the effect of hydrothermal pretreatment by scanning electron microscope (SEM) and X-ray diffraction (XRD

  2. In vitro and in vivo characteristics of a human colon cancer cell line, SNU-C5N, expressing sodium-iodide symporter

    International Nuclear Information System (INIS)

    Min, Jung-Jun; Chung, June-Key; Jin Lee, Yong; Hoon Shin, Jae; Seok Yeo, Jeong; Min Jeong, Jae; Bom, Dong Soo Leea Hee-Seung; Chul Lee, Myung

    2002-01-01

    Rat NIS (rNIS) genes were transfected into a human colon cancer cell line (SNU-C5) by lipofection. The transfected cells (SNU-C5N) exhibited an increase 125 I uptake to a level 10 times higher than the untransfected SNU-C5 cells. The addition of 300 μM DIDS, an anion channel blocker, to the culture media led to a 2.35 times increase of 125 I uptake in the cells. For the first 10 minutes, up to 70% of the cellular radioactivity was released into the medium. In the biodistribution study using SNU-C5N-xenografted mice, the %ID/g of the SNU-C5N tumors at 1, 3, 6, and 12 h after the 125 I injection were 4.43%, 1.09%, 1.05%, and 0.05%, respectively, which were significantly higher than those for the SNU-C5 tumors (P<0.05). In tumor imaging, the SNU-C5N-xenografted tumor was clearly visible. In this study, NIS lipofection is efficient for triggering significant iodide uptake by a nonthyroidal tumor. However, for an increased therapeutic effect, the key issue is iodide retention in the target tissue

  3. In vitro and in vivo characteristics of a human colon cancer cell line, SNU-C5N, expressing sodium-iodide symporter

    Energy Technology Data Exchange (ETDEWEB)

    Min, Jung-Jun; Chung, June-Key E-mail: jkchung@plaza.snu.ac.kr; Jin Lee, Yong; Hoon Shin, Jae; Seok Yeo, Jeong; Min Jeong, Jae; Bom, Dong Soo Leea Hee-Seung; Chul Lee, Myung

    2002-07-01

    Rat NIS (rNIS) genes were transfected into a human colon cancer cell line (SNU-C5) by lipofection. The transfected cells (SNU-C5N) exhibited an increase {sup 125}I uptake to a level 10 times higher than the untransfected SNU-C5 cells. The addition of 300 {mu}M DIDS, an anion channel blocker, to the culture media led to a 2.35 times increase of {sup 125}I uptake in the cells. For the first 10 minutes, up to 70% of the cellular radioactivity was released into the medium. In the biodistribution study using SNU-C5N-xenografted mice, the %ID/g of the SNU-C5N tumors at 1, 3, 6, and 12 h after the {sup 125}I injection were 4.43%, 1.09%, 1.05%, and 0.05%, respectively, which were significantly higher than those for the SNU-C5 tumors (P<0.05). In tumor imaging, the SNU-C5N-xenografted tumor was clearly visible. In this study, NIS lipofection is efficient for triggering significant iodide uptake by a nonthyroidal tumor. However, for an increased therapeutic effect, the key issue is iodide retention in the target tissue.

  4. Simulation of the «COSMONAUT-ROBOT» System Interaction on the Lunar Surface Based on Methods of Machine Vision and Computer Graphics

    Science.gov (United States)

    Kryuchkov, B. I.; Usov, V. M.; Chertopolokhov, V. A.; Ronzhin, A. L.; Karpov, A. A.

    2017-05-01

    Extravehicular activity (EVA) on the lunar surface, necessary for the future exploration of the Moon, involves extensive use of robots. One of the factors of safe EVA is a proper interaction between cosmonauts and robots in extreme environments. This requires a simple and natural man-machine interface, e.g. multimodal contactless interface based on recognition of gestures and cosmonaut's poses. When travelling in the "Follow Me" mode (master/slave), a robot uses onboard tools for tracking cosmonaut's position and movements, and on the basis of these data builds its itinerary. The interaction in the system "cosmonaut-robot" on the lunar surface is significantly different from that on the Earth surface. For example, a man, dressed in a space suit, has limited fine motor skills. In addition, EVA is quite tiring for the cosmonauts, and a tired human being less accurately performs movements and often makes mistakes. All this leads to new requirements for the convenient use of the man-machine interface designed for EVA. To improve the reliability and stability of human-robot communication it is necessary to provide options for duplicating commands at the task stages and gesture recognition. New tools and techniques for space missions must be examined at the first stage of works in laboratory conditions, and then in field tests (proof tests at the site of application). The article analyzes the methods of detection and tracking of movements and gesture recognition of the cosmonaut during EVA, which can be used for the design of human-machine interface. A scenario for testing these methods by constructing a virtual environment simulating EVA on the lunar surface is proposed. Simulation involves environment visualization and modeling of the use of the "vision" of the robot to track a moving cosmonaut dressed in a spacesuit.

  5. The Crystal Structure of Cobra Venom Factor, a Cofactor for C3- and C5-Convertase CVFBb

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, Vengadesan; Ponnuraj, Karthe; Xu, Yuanyuan; Macon, Kevin; Volanakis, John E.; Narayana, Sthanam V.L.; (Madras); (UAB)

    2009-05-26

    Cobra venom factor (CVF) is a functional analog of human complement component C3b, the active fragment of C3. Similar to C3b, in human and mammalian serum, CVF binds factor B, which is then cleaved by factor D, giving rise to the CVFBb complex that targets the same scissile bond in C3 as the authentic complement convertases C4bC2a and C3bBb. Unlike the latter, CVFBb is a stable complex and an efficient C5 convertase. We solved the crystal structure of CVF, isolated from Naja naja kouthia venom, at 2.6 {angstrom} resolution. The CVF crystal structure, an intermediate between C3b and C3c, lacks the TED domain and has the CUB domain in an identical position to that seen in C3b. The similarly positioned CUB and slightly displaced C345c domains of CVF could play a vital role in the formation of C3 convertases by providing important primary binding sites for factor B.

  6. The behaviour of organic solvents containing C5-BTBP and CyMe4-BTBP at low irradiation doses

    International Nuclear Information System (INIS)

    Retegan, T.; Fermvik, A.; Skarnemark, G.; Foreman, M.R.S.

    2007-01-01

    Low doses of gamma radiation were given to four different solvents containing C5-BTBP and CyMe 4 -BTBP, each molecule dissolved both in cyclohexanone and hexanol. Four corresponding solvents were kept unirradiated and used as references for the extraction experiments. Multiple samples were taken from both the irradiated solutions and the reference solutions at certain time intervals. The samples were used in extraction experiments with the radionuclides 241 Am and 152 Eu. The protection against radiolysis of the extracting molecules by the diluent used for dissolution without adding a scavenger molecule was checked. The interplay between the diluent and the side group of the extracting molecule for protection against radiolysis was also studied by keeping the same type of core molecule for binding to the metal ions and varying the diluent and side group. The results were unexpected. The presence of a cyclic molecule as both a side group or diluent seems to keep the extraction of europium almost unaffected by radiolysis, while americium behaves differently from solvent to solvent. The diluent alone does not protect the extracting molecule. In some of the studied systems there is a distinct change in the extraction behaviour of Am between the irradiated and reference solutions, an effect that is however only present at the beginning of the experimental series. At later times the difference in distribution ratios between the irradiated and reference solution is constant. This phenomenon is found only when the side group and diluent are structurally dissimilar. (orig.)

  7. Photochemical Formation of C1-C5 Alkyl Nitrates in Suburban Hong Kong and over the South China Sea.

    Science.gov (United States)

    Zeng, Lewei; Lyu, Xiaopu; Guo, Hai; Zou, Shichun; Ling, Zhenhao

    2018-04-24

    Alkyl nitrates (RONO 2 ) are important reservoirs of atmospheric nitrogen, regulating nitrogen cycling and ozone (O 3 ) formation. In this study, we found that propane and n-butane were significantly lower at the offshore site (WSI) in Hong Kong ( p 0.05). Stronger oxidative capacity at WSI led to more efficient RONO 2 formation. Relative incremental reactivity (RIR) was for the first time used to evaluate RONO 2 -precursor relationships. In contrast to a consistently volatile organic compounds (VOC)-limited regime at TC, RONO 2 formation at WSI switched from VOC-limited regime during O 3 episodes to VOC and nitrogen oxides (NO x ) colimited regime during nonepisodes. Furthermore, unlike the predominant contributions of parent hydrocarbons to C 4 -C 5 RONO 2 , the production of C 1 -C 3 RONO 2 was more sensitive to other VOCs like aromatics and carbonyls, which accounted for ∼40-90% of the productions of C 1 -C 3 alkylperoxy (RO 2 ) and alkoxy radicals (RO) at both sites. This resulted from the decomposition of larger RO 2 /RO and the change of OH abundance under the photochemistry of other VOCs. This study advanced our understanding of the photochemical formation of RONO 2 , particularly the relationships between RONO 2 and their precursors, which were not confined to the parent hydrocarbons.

  8. Results of postirradiation examination of the in-pile blockage experiments MOL-7C/4 and MOL-7C/5

    International Nuclear Information System (INIS)

    Weimar, P.; Schleisiek, K.

    1991-01-01

    The Mol-7C in-pile local blockage experiments are performed in the BR-2 reactor at Mol, Belgium as a joint project of Kernforchungszentrum Karlsruhe (KfK) and Studiecentrum voor Kernenergie/Centre d'Etude de l'Energie Nuclearire-Mol. The main objective is to investigate the consequences of local cooling disturbances in liquid-metal-cooled reactor (LMR) fuel subassemblies. In the tests Mol-7C/4 and MOL-7C/5, fuel pins from KNK II are used with a burnup of 5 and 1.7%, respectively. An active central porous blockage is used to simulate the cooling disturbance. During irradiation, the blockage causes significant local damage, including melting of cladding and fuel. Extensive postirradiation examinations (PIE) are performed to investigate the extent of damage. In this paper a description and interpretation of results of the destructive PIE performed at the Hot Cells Laboratory at KfK is given, along with some conclusions related to LMR safety

  9. Paleontology and stratigraphy of the Upper Triassic Kamishak Formation in the Puale Bay-Cape Kekurnoi-Alinchak Bay area, Karluk C-4 and C-5 quadrangle

    Data.gov (United States)

    Department of the Interior — This report summarizes the paleontological character and stratigraphy of the Kamishak Formation in the Puale Bay–Cape Kekurnoi–Alinchak Bay area, Karluk C-4 and C-5...

  10. The comparison of MODIS-Aqua (C5 and CALIOP (V2 & V3 aerosol optical depth

    Directory of Open Access Journals (Sweden)

    J. Redemann

    2012-03-01

    Full Text Available We assess the consistency between instantaneously collocated level-2 aerosol optical depth (AOD retrievals from MODIS-Aqua (C5 and CALIOP (Version 2 & 3, comparing the standard MODIS AOD (MYD04_L2 data to the AOD calculated from CALIOP aerosol extinction profiles for both the previous release (V2 and the latest release (V3 of CALIOP data. Based on data collected in January 2007, we investigate the most useful criteria for screening the MODIS and CALIOP retrievals to achieve the best agreement between the two data sets. Applying these criteria to eight months of data (Jan, Apr, Jul, Oct 2007 and 2009, we find an order of magnitude increase for the CALIOP V3 data density (by comparison to V2, that is generally accompanied by equal or better agreement with MODIS AOD. Differences in global, monthly mean, over-ocean AOD (532 nm between CALIOP and MODIS range between 0.03 and 0.04 for CALIOP V3, with CALIOP generally biased low, when all available data from both sensors are considered. Root-mean-squares (RMS differences in instantaneously collocated AOD retrievals by the two instruments are reduced from values ranging between 0.14 and 0.19 using the unscreened V3 data to values ranging from 0.09 to 0.1 for the screened data. A restriction to scenes with cloud fractions less than 1% (as defined in the MODIS aerosol retrievals generally results in improved correlation (R2>0.5, except for the month of July when correlations remain relatively lower. Regional assessments show hot spots in disagreement between the two sensors in Asian outflow during April and off the coast of South Africa in July.

  11. Geologic Emissions of Methane and C2 - C5 Alkanes at the La Brea Tar Pits, Los Angeles, CA

    Science.gov (United States)

    Doezema, L. A.; Etiope, G.; Pacheco, C.

    2017-12-01

    Natural hydrocarbon (oil and gas) seeps are widespread in Los Angeles due to gas migration, along faults, from numerous subsurface petroleum fields. These seeps may represent important natural contributors of methane (CH4) and heavier alkanes (C2-C4) for the atmosphere. Methane flux measurements were made from various locations at the La Brea Tar Pits in Los Angeles, CA. Measurements were made using a closed-chamber method and spectroscopic sensors for CH4 and CO2, at 26 oil-asphalt seeps and 188 other sites, without gas manifestations, homogeneously distributed throughout the park. The molecular C1 - C5 composition of gas released from seeps and soil was also analyzed using either FTIR spectroscopy or gas chromatography (GC-FID). Methane emissions from seeps varied from approximately 7 to 54,000 g m-2 day-1, while emissions from soil degassing were between 0 and 9,000 g m-2 day-1. Total emissions were estimated to be in the order of 103 kg day-1 for methane, and at least 10 and 5 kg day-1 for ethane and propane, respectively. The seeping gas exhibited high C1/(C2 + C3) ratios, likely due to molecular fractionation during gas migration from a subsurface petroleum reservoir. Evidence for biodegredation in certain samples was indicated by large i-butane to n-butane ratios. These molecular alterations can be important tracers of natural seepage and should be considered in the atmospheric modelling of the relative contribution of fossil fuel (anthropogenic fugitive emission and natural geologic sources) vs biogenic sources, on local and global scales.

  12. Standard Molar Enthalpy of Formation of RE(C5H8NS2)3(o-phen)

    Institute of Scientific and Technical Information of China (English)

    MENG Xiang-Xin; GAO Sheng-Li; CHEN San-Ping; YANG Xu-Wu; XIE Gang; SHI Qi-Zhen

    2005-01-01

    Five solid ternary complexes of RE(C5H8NS2)3(o-phen) (RE=Ho, Er, Tm, Yb, Lu) have been synthesized in absolute ethanol by rare earth chloride low hydrate reacting with the mixed ligands of ammonium pyrrolidinedithiocarbamate (APDC) and 1,10-phenanthroline·H2O (o-phen·H2O) in the ordinary laboratory atmosphere without any cautions against moisture or air. IR spectra of the complexes showed that the RE3+ coordinated with six sulfur atoms of three PDC- and two nitrogen atoms of o-phen·H2O. It was assumed that the coordination number of RE3+was eight. The constant-volume combustion energies of the complexes, △cU, were determined as (-16788.46±7.74), (- 15434.53± 8.28), (- 15287.807.31), (- 15200.50±7.22) and (- 15254.34±6.61) kJ·mol-1, respectively, by a precise rotating-bomb calorimeter at 298.15 K. Its standard molar enthalpies of combustion, △cH m,and standard molar enthalpies of formation, △fH m, were calculated as (-16803.95 ±7.74), (-15450.02±8.28),(-15303.29±9.28), (-15215.99±7.22), (-15269.83±6.61) kJ·mol-1 and (-1115.42±8.94), (-2477.80±9.15), (-2619.95 ±10.44), (-2670.17 ± 8.22), ( -2650.06± 8.49) kJ·mol-1, respectively.

  13. Kinetic study of the degradation of C5 and C6 unsaturated aldehydes and alcohols by ozone

    Science.gov (United States)

    Kalalian, Carmen; Roth, Estelle; Chakir, Abdelkhaleq

    2017-04-01

    Emissions of biogenic volatile organic compounds (VOCs) are higher than those from anthropogenic sources. They are therefore likely to have a great influence on atmospheric chemistry both locally and regionally, through their impact on the HOx balance (HOx = HO + HO2), ozone production and ability to form secondary organic aerosols (SOA). Among the volatile organic compounds of biogenic origin are the family of C5 and C6 unsaturated aldehydes and alcohols. Few information exist regarding the fate of these compounds in the atmosphere especially there reaction with ozone. In this work, we studied the kinetics of the reaction of three unsaturated aldehydes (trans-2-pentenal, trans-2-hexenal and 2-methyl-2-pentenal) and three unsaturated alcohols (1-penten-3-ol, cis-2-penten-1-ol and trans-3-hexen-1-ol) with ozone O3 in a rigid atmospheric simulation chamber coupled to an FTIR spectrometer at four different temperatures (273, 298, 333 and 353 K) and at atmospheric pressure. The rate constants of the ozonolysis reaction of the unsaturated aldehydes and the unsaturated alcohols studied were determined and the following Arrhenius expression was obtained (cm3 molecule -1 s -1): k (Trans -2-pentenal)= (3.83 ± 3.71) x 10-16 exp (- (1706 ± 295) / T) k (Trans-2-hexenal)= (1.43 ± 0.67) x 10-16 exp (- (1369 ± 141) / T) k(2-Methyl-2-pentenal)= (3.62± 0.22) x 10-18 exp (- (121 ± 20) / T) k(1-penten-3-ol) = (1.42 ± 1.24) x 10-16 exp (- (642 ± 250) / T) k(Cis-2-penten-1-ol)= (3.14 ± 0.45) x 10-15 exp (- (1045 ± 40) / T) k(Trans-3-hexen-1-ol)= (6.38 ± 1.75) x 10-16 exp (- (686 ± 89) / T) The obtained data will be discussed in terms of structure-reactivity relationship and compared with the reported reactivity with OH radicals. The atmospheric implications derived from this study are discussed as well.

  14. Effects of the η(5)-C5H4(i)Pr Ligand on the Properties Exhibited by Its Tungsten Nitrosyl Complexes.

    Science.gov (United States)

    Fabulyak, Diana; Baillie, Rhett A; Patrick, Brian O; Legzdins, Peter; Rosenfeld, Devon C

    2016-02-15

    Reaction of Na[η(5)-C5H4(i)Pr] with W(CO)6 in refluxing THF for 4 days generates a solution of Na[(η(5)-C5H4(i)Pr)W(CO)3] that when treated with N-methyl-N-nitroso-p-toluenesulfonamide at ambient temperatures affords (η(5)-C5H4(i)Pr)W(NO)(CO)2 (1) that is isolable in good yield as an analytically pure orange oil. Treatment of 1 with an equimolar amount of I2 in Et2O at ambient temperatures affords (η(5)-C5H4(i)Pr)W(NO)I2 (2) as a dark brown solid in excellent yield. Sequential treatment at low temperatures of 2 with 0.5 equiv of Mg(CH2CMe3)2 and Mg(CH2CH═CMe2)2 in Et2O produces the alkyl allyl complex, (η(5)-C5H4(i)Pr)W(NO)(CH2CMe3)(η(3)-CH2CHCMe2) (3), as a thermally sensitive yellow liquid. Complex 3 may also be synthesized, albeit in low yield, in one vessel at low temperatures by reacting 1 first with 1 equiv of PCl5 and then with the binary magnesium reagents specified above. Interestingly, similar treatment of 1 in Et2O with PCl5 and only 0.5 equiv of Mg(CH2CH═CMe2)2 results in the formation of the unusual complex (η(5)-C5H4(i)Pr)W(NO)(PCl2CMe2CH═CH2)Cl2 (4), which probably is formed via a metathesis reaction of the binary magnesium reagent with (η(5)-C5H4(i)Pr)W(NO)(PCl3)Cl2. The C-D activation of C6D6 by complex 3 has been investigated and compared to that exhibited by its η(5)-C5Me5, η(5)-C5Me4H, and η(5)-C5Me4(n)Pr analogues. Kinetic analyses of the various activations have established that the presence of the η(5)-C5H4(i)Pr ligand significantly increases the rate of the reaction, an outcome that can be attributed to a combination of steric and electronic factors. In addition, mechanistic studies have established that in solution 3 loses neopentane under ambient conditions to generate exclusively the 16e η(2)-diene intermediate complex (η(5)-C5H4(i)Pr)W(NO)(η(2)-CH2═CMeCH═CH2), which then effects the subsequent C-D activations. This behavior contrasts with that exhibited by the η(5)-C5Me5 analogue of 3 which forms both η(2

  15. The methyltransferase NSD3 has chromatin-binding motifs, PHD5-C5HCH, that are distinct from other NSD (nuclear receptor SET domain) family members in their histone H3 recognition.

    Science.gov (United States)

    He, Chao; Li, Fudong; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu

    2013-02-15

    The NSD (nuclear receptor SET domain-containing) family members, consisting of NSD1, NSD2 (MMSET/WHSC1), and NSD3 (WHSC1L1), are SET domain-containing methyltransferases and aberrant expression of each member has been implicated in multiple diseases. They have specific mono- and dimethylase activities for H3K36, whereas play nonredundant roles during development. Aside from the well characterized catalytic SET domain, NSD proteins have multiple potential chromatin-binding motifs that are clinically relevant, including the fifth plant homeodomain (PHD5) and the adjacent Cys-His-rich domain (C5HCH) located at the C terminus. Herein, we report the crystal structures of the PHD5-C5HCH module of NSD3, in the free state and in complex with H3(1-7) (H3 residues 1-7), H3(1-15) (H3 residues 1-15), and H3(1-15)K9me3 (H3 residues 1-15 with trimethylation on K9) peptides. These structures reveal that the PHD5 and C5HCH domains fold into a novel integrated PHD-PHD-like structural module with H3 peptide bound only on the surface of PHD5 and provide the molecular basis for the recognition of unmodified H3K4 and trimethylated H3K9 by NSD3 PHD5. Structural studies and binding assays show that differences exist in histone binding specificity of the PHD5 domain between three members of the NSD family. For NSD2, the PHD5-C5HCH:H3 N terminus interaction is largely conserved, although with a stronger preference for unmethylated H3K9 (H3K9me0) than trimethylated H3K9 (H3K9me3), and NSD1 PHD5-C5HCH does not bind to H3 peptides. Our results shed light on how NSD proteins that mediate H3K36 methylation are localized to specific genomic sites and provide implications for the mechanism of functional diversity of NSD proteins.

  16. Classification and Progression Based on CFS-GA and C5.0 Boost Decision Tree of TCM Zheng in Chronic Hepatitis B.

    Science.gov (United States)

    Chen, Xiao Yu; Ma, Li Zhuang; Chu, Na; Zhou, Min; Hu, Yiyang

    2013-01-01

    Chronic hepatitis B (CHB) is a serious public health problem, and Traditional Chinese Medicine (TCM) plays an important role in the control and treatment for CHB. In the treatment of TCM, zheng discrimination is the most important step. In this paper, an approach based on CFS-GA (Correlation based Feature Selection and Genetic Algorithm) and C5.0 boost decision tree is used for zheng classification and progression in the TCM treatment of CHB. The CFS-GA performs better than the typical method of CFS. By CFS-GA, the acquired attribute subset is classified by C5.0 boost decision tree for TCM zheng classification of CHB, and C5.0 decision tree outperforms two typical decision trees of NBTree and REPTree on CFS-GA, CFS, and nonselection in comparison. Based on the critical indicators from C5.0 decision tree, important lab indicators in zheng progression are obtained by the method of stepwise discriminant analysis for expressing TCM zhengs in CHB, and alterations of the important indicators are also analyzed in zheng progression. In conclusion, all the three decision trees perform better on CFS-GA than on CFS and nonselection, and C5.0 decision tree outperforms the two typical decision trees both on attribute selection and nonselection.

  17. c-C5H5 on a Ni(1 1 1) surface: Theoretical study of the adsorption, electronic structure and bonding

    International Nuclear Information System (INIS)

    German, E.; Simonetti, S.; Pronsato, E.; Juan, A.; Brizuela, G.

    2008-01-01

    In the present work the ASED-MO method is applied to study the adsorption of cyclopentadienyl anion on a Ni(1 1 1) surface. The adsorption with the centre of the aromatic ring placed above the hollow position has been identified to be energetically the most favourable. The aromatic ring remains almost flat, the H atoms are tilted 17 deg. away from the metal surface. We modelled the metal surface by a two-dimensional slab of finite thickness, with an overlayer of c-C 5 H 5 - , one c-C 5 H 5 - per nine surface Ni atoms. The c-C 5 H 5 - molecule is attached to the surface with its five C atoms bonding mainly with three Ni atoms. The Ni-Ni bond in the underlying surface and the C-C bonds of c-C 5 H 5 - are weakened upon adsorption. We found that the band of Ni 5d z 2 orbitals plays an important role in the bonding between c-C 5 H 5 - and the surface, as do the Ni 6s and 6p z bands

  18. State-of-the-art Theory Application

    Science.gov (United States)

    Gully, S.

    1983-01-01

    Nonlinear failure detection and isolation (FDI), robust FDI, coordinate controller, detector, estimator, and isolator, AI/modern systems theory, sensor fusion, and man-machine interaction are addressed.

  19. Etching Enhancement Followed by Nitridation on Low-k SiOCH Film in Ar/C5F10O Plasma

    Science.gov (United States)

    Miyawaki, Yudai; Shibata, Emi; Kondo, Yusuke; Takeda, Keigo; Kondo, Hiroki; Ishikawa, Kenji; Okamoto, Hidekazu; Sekine, Makoto; Hori, Masaru

    2013-02-01

    The etching rates of low-dielectric-constant (low-k), porous SiOCH (p-SiOCH) films were increased by nitrogen-added Ar/C5F10O plasma etching in dual-frequency (60 MHz/2 MHz)-excited parallel plate capacitively coupled plasma. Previously, perfluoropropyl vinyl ether [C5F10O] provided a very high density of CF3+ ions [Nagai et al.: Jpn. J. Appl. Phys. 45 (2006) 7100]. Surface nitridation on the p-SiOCH surface exposed to Ar/N2 plasma led to the etching of larger amounts of p-SiOCH in Ar/C5F10O plasma, which depended on the formation of bonds such as =C(sp2)=N(sp2)- and -C(sp)≡N(sp).

  20. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration

    Directory of Open Access Journals (Sweden)

    Klein Michael L

    2011-07-01

    Full Text Available Abstract Background Age related macular degeneration (AMD is the leading cause of irreversible blindness in elderly populations worldwide. Inflammation, among many factors, has been suggested to play an important role in AMD pathogenesis. Recent studies have demonstrated a strong genetic association between AMD and complement factor H (CFH, the down-regulatory factor of complement activation. Elevated levels of complement activating molecules including complement component 5a (C5a have been found in the serum of AMD patients. Our aim is to study whether C5a can impact human T cells and its implication in AMD. Methods Human peripheral blood mononuclear cells (PBMCs were isolated from the blood of exudative form of AMD patients using a Ficoll gradient centrifugation protocol. Intracellular staining and enzyme-linked immunosorbent assays were used to measure protein expression. Apoptotic cells were detected by staining of cells with the annexin-V and TUNEL technology and analyzed by a FACS Caliber flow cytometer. SNP genotyping was analyzed by TaqMan genotyping assay using the Real-time PCR system 7500. Results We show that C5a promotes interleukin (IL-22 and IL-17 expression by human CD4+ T cells. This effect is dependent on B7, IL-1β and IL-6 expression from monocytes. We have also found that C5a could protect human CD4+ cells from undergoing apoptosis. Importantly, consistent with a role of C5a in promoting IL-22 and IL-17 expression, significant elevation in IL-22 and IL-17 levels was found in AMD patients as compared to non-AMD controls. Conclusions Our results support the notion that C5a may be one of the factors contributing to the elevated serum IL-22 and IL-17 levels in AMD patients. The possible involvement of IL-22 and IL-17 in the inflammation that contributes to AMD may herald a new approach to treat AMD.

  1. Low-k SiOCH Film Etching Process and Its Diagnostics Employing Ar/C5F10O/N2 Plasma

    Science.gov (United States)

    Nagai, Mikio; Hayashi, Takayuki; Hori, Masaru; Okamoto, Hidekazu

    2006-09-01

    We proposed an environmental harmonic etching gas of C5F10O (CF3CF2CF2OCFCF2), and demonstrated the etching of low-k SiOCH films employing a dual-frequency capacitively coupled etching system. Dissociative ionization cross sections for the electron impact ionizations of C5F10O and c-C4F8 gases have been measured by quadrupole mass spectroscopy (QMS). The dissociative ionization cross section of CF3+ from C5F10O gas was much higher than those of other ionic species, and 10 times higher than that of CF3+ from C4F8 gas. CF3+ is effective for increasing the etching rate of SiO2. As a result, the etching rate of SiOCH films using Ar/C5F10O/N2 plasma was about 1000 nm/min, which is much higher than that using Ar/C4F8/N2 plasma. The behaviours of fluorocarbon radicals in Ar/C5F10O/N2 plasma, which were measured by infrared diode laser absorption spectroscopy, were similar to those in Ar/C4F8/N2 plasma. The densities of CF and CF3 radicals were markedly decreased with increasing N2 flow rate. Etching rate was controlled by N2 flow rate. A vertical profile of SiOCH with a high etching rate and less microloading was realized using Ar/C5F10O/N2 plasma chemistry.

  2. Evolution and estimated age of the C5 Lukala carbonate-evaporite ramp complex in the Lower Congo region (Democratic Republic of Congo): New perspectives in Central Africa

    Science.gov (United States)

    Delpomdor, F.; Van Vliet, N.; Devleeschouwer, X.; Tack, L.; Préat, A.

    2018-01-01

    New detailed lithological, sedimentological, chemostratigraphic data were obtained from exploration drilling samples on the C5 carbonate-dominated formation of the Neoproterozoic Lukala Subgroup (former Schisto-Calcaire Subgroup) from the West Congo Belt (WCB) in the Democratic Republic of Congo. This formation records the last post-Marinoan sea-level events that occurred in the whole basin, followed by the development of the Araçuaï-West Congo Orogen between 630 and 560 Ma. The C5 Formation consists of back-reef lagoonal and peritidal/sabkha cycles of ∼2.0 m in thickness, that record a short-time marine regression, rapidly flooded by a marine transgression with deposition of organic-rich argillaceous carbonates or shales under dysoxia and anoxia conditions. These dysoxic/anoxic waters were rapidly followed by a regional-scale marine transgression, favouring mixing with well-oxygenated waters, and the development of benthic Tonian to Cambro-Ordovician Obruchevella parva-type 'seagrasses' in the nearshore zones of the lagoons. New δ13C and 87Sr/86Sr isotopic data in the C5 Formation of the Lukala Subgroup are used in the frame of a correlation with the Sete Lagoas Formation in Brazil. Relatively comparable negative to positive δ13C excursions point to marine flooding of the whole basin and allow extension of the debatable Late Ediacaran age of the uppermost Sete Lagoas and C5 formations. Sr isotope ;blind dating; failed due to low Sr concentration related to a dolomitization event close 540 Ma. Several tentative datings of the C5 Formation converge to a Late Ediacaran age ranging between 575 and 540 Ma. As the overlying Mpioka folded Subgroup, the C5 series suffered the Pan African deformation, dated at 566 ± 42 Ma. Unlike the previously generally accepted interpretation, our data suggests that the Mpioka Subgroup was deposited in the Early Cambrian.

  3. Relative bioavailability of 13C5-folic acid in pectin-coated folate fortified rice in humans using stable isotope techniques.

    Science.gov (United States)

    de Ambrosis, A; Vishnumohan, S; Paterson, J; Haber, P; Arcot, J

    2017-01-01

    The aim of the study was to measure the relative bioavailability of labeled pteroylglutamic acid (13C5-PteGlu) from a pectin-coated fortified rice in vivo to measure any effect of the edible coating on folic acid bioavailability. Healthy volunteers (N=26) aged 18-39 years received three test meals in three randomized short-term cross-over trials: Trial 1: aqueous 400 μg 13C5-PteGlu, Trial 2: 200 g cooked white rice+400 μg 13C5-PteGlu,Trial 3: 200 g fortified cooked white rice with pectin-coated premix containing 400 μg 13C5-PteGlu. Blood samples were drawn at 0,1,2,5 and 8 h postprandial. The concentration of 13C5-5 methyl-tetrahydrofolate appearing in plasma was quantified using high performance liquid chromatography-mass spectrometry (MS)/MS. For 24 h before baseline estimation and during the area under the curve (AUC) study, the subjects were placed on a low folate diet (∼100 μg/day). The relative bioavailability of the folic acid following Trial 3 was measured by comparing the 13C5-5 methyl-tetrahydrofuran (THF) AUC with Trials 1 and 2. The bioavailability of folic acid in a pectin-coated rice premix was 68.7% (range 47-105) and 86.5% (range 65-115) in uncoated fortified rice relative to aqueous folic acid. This study is the first demonstration of the bioavailability of folate in pectin-coated fortified rice in humans.

  4. Generation of a command language for nuclear signal and image processing on the basis of a general interactive system

    International Nuclear Information System (INIS)

    Pretschner, D.P.; Pfeiffer, G.; Deutsches Elektronen-Sychnchrotron

    1981-01-01

    In the field of nuclear medicine, BASIC and FORTRAN are currently being favoured as higher-level programming languages for computer-aided signal processing, and most operating systems of so-called ''freely programmable analyzers'' in nuclear wards have compilers for this purpose. However, FORTRAN is not an interactive language and thus not suited for conversational computing as a man-machine interface. BASIC, on the other hand, although a useful starting language for beginners, is not sufficiently sophisticated for complex nuclear medicine problems involving detailed calculations. Integration of new methods of signal acquisition, processing and presentation into an existing system or generation of new systems is difficult in FORTRAN, BASIC or ASSEMBLER and can only be done by system specialists, not by nuclear physicians. This problem may be solved by suitable interactive systems that are easy to learn, flexible, transparent and user-friendly. An interactive system of this type, XDS, was developed in the course of a project on evaluation of radiological image sequences. An XDS-generated command processing system for signal and image processing in nuclear medicine is described. The system is characterized by interactive program development and execution, problem-relevant data types, a flexible procedure concept and an integrated system implementation language for modern image processing algorithms. The advantages of the interactive system are illustrated by an example of diagnosis by nuclear methods. (orig.) [de

  5. Total Synthesis of Zoanthamine Alkaloids, Part 2. Construction of the C1-C5, C6-C10 and C11-C24 Fragments of Zoanthamine

    DEFF Research Database (Denmark)

    Tanner, David Ackland; Tedenborg, Lars; Somfai, Peter

    1997-01-01

    This paper describes the construction of three key intermediates for a projected total synthesis of the marine alkaloid zoanthamine. These building blocks, corresponding to the C1-C5, C6-C10 and C11-C24 fragments of the target molecule, are synthesised efficiently form (R)-hydroxymethyl-butyrolac......This paper describes the construction of three key intermediates for a projected total synthesis of the marine alkaloid zoanthamine. These building blocks, corresponding to the C1-C5, C6-C10 and C11-C24 fragments of the target molecule, are synthesised efficiently form (R...

  6. From laboratory catalysts to a new prototype: a novel real candidate for the isomerization of C5–C6 paraffins

    Czech Academy of Sciences Publication Activity Database

    Hidalgo, J. M.; Kaucký, Dalibor; Bortnovsky, O.; Černý, R.; Sobalík, Zdeněk

    2015-01-01

    Roč. 5, JUN 2015 (2015), s. 56625-56628 ISSN 2046-2069 R&D Projects: GA MPO FR-TI3/316 Institutional support: RVO:61388955 Keywords : C5-C6 paraffins * isomeration * Pt/WO3–ZrO2 materials Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.289, year: 2015

  7. Systems biology and pathway engineering enable Saccharomyces cerevisiae to utilize C-5 and C-6 sugars simultaneously for cellulosic ethanol production

    Science.gov (United States)

    Saccharomyces cerevisiae is a traditional industrial workhorse for ethanol production. However, conventional ethanologenic yeast is superior in fermentation of hexose sugars (C-6) such as glucose but unable to utilize pentose sugars (C-5) such as xylose richly embedded in lignocellulosic biomass. In...

  8. Total Synthesis of Zoanthamine Alkaloids, Part 2. Construction of the C1-C5, C6-C10 and C11-C24 Fragments of Zoanthamine

    DEFF Research Database (Denmark)

    Tanner, David Ackland; Tedenborg, Lars; Somfai, Peter

    1997-01-01

    This paper describes the construction of three key intermediates for a projected total synthesis of the marine alkaloid zoanthamine. These building blocks, corresponding to the C1-C5, C6-C10 and C11-C24 fragments of the target molecule, are synthesised efficiently form (R...

  9. Neuroimaging findings in Joubert syndrome with C5orf42 gene mutations: A milder form of molar tooth sign and vermian hypoplasia.

    Science.gov (United States)

    Enokizono, Mikako; Aida, Noriko; Niwa, Tetsu; Osaka, Hitoshi; Naruto, Takuya; Kurosawa, Kenji; Ohba, Chihiro; Suzuki, Toshifumi; Saitsu, Hirotomo; Goto, Tomohide; Matsumoto, Naomichi

    2017-05-15

    Little is known regarding neuroimaging-genotype correlations in Joubert syndrome (JBTS). To elucidate one of these correlations, we investigated the neuroimaging findings of JBTS patients with C5orf42 mutations. Neuroimaging findings in five JBTS patients with C5orf42 mutations were retrospectively assessed with regard to the infratentorial and supratentorial structures on T1-magnetization prepared rapid gradient echo (MPRAGE), T2-weighted images, and color-coded fractional anisotropy (FA) maps; the findings were compared to those in four JBTS patients with mutations in other genes (including three with AHI1 and one with TMEM67 mutations). In C5orf42-mutant patients, the infratentorial magnetic resonance (MR) images showed normal or minimally thickened and minimally elongated superior cerebellar peduncles (SCP), normal or minimally deepened interpeduncular fossa (IF), and mild vermian hypoplasia (VH). However, in other patients, all had severe abnormalities in the SCP and IF, and moderate to marked VH. Supratentorial abnormalities were found in one individual in other JBTS. In JBTS with all mutations, color-coded FA maps showed the absence of decussation of the SCP (DSCP). The morphological neuroimaging findings in C5orf42-mutant JBTS were distinctly mild and made diagnosis difficult. However, the absence of DSCP on color-coded FA maps may facilitate the diagnosis of JBTS. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Treatment with anti-C5aR mAb leads to early-onset clinical and mechanistic effects in the murine delayed-type hypersensitivity arthritis model

    DEFF Research Database (Denmark)

    Atkinson, Sara Marie; Nansen, Anneline; Usher, Pernille A.

    2015-01-01

    Blockade of the complement cascade at the C5a/C5a receptor (C5aR)-axis is believed to be an attractive treatment avenue in rheumatoid arthritis (RA). However, the effects of such interventions during the early phases of arthritis remain to be clarified. In this study we use the murine delayed-typ...

  11. Synthesis, crystal structure, growth, optical and third order nonlinear optical studies of 8HQ2C5N single crystal - An efficient third-order nonlinear optical material

    Energy Technology Data Exchange (ETDEWEB)

    Divya Bharathi, M.; Ahila, G.; Mohana, J. [Department of Physics, Presidency College, Chennai 600005 (India); Chakkaravarthi, G. [Department of Physics, CPCL Polytechnic College, Chennai 600068 (India); Anbalagan, G., E-mail: anbu24663@yahoo.co.in [Department of Nuclear Physics, University of Madras, Chennai 600025 (India)

    2017-05-01

    A neoteric organic third order nonlinear optical material 8-hydroxyquinolinium 2-chloro-5-nitrobenzoate dihydrate (8HQ2C5N) was grown by slow cooling technique using ethanol: water (1:1) mixed solvent. The calculated low value of average etch pit solidity (4.12 × 10{sup 3} cm{sup −2}) indicated that the title crystal contain less defects. From the single crystal X-ray diffraction data, it was endowed that 8HQ2C5N crystal belongs to the monoclinic system with centrosymmetric space group P2{sub 1}/c and the cell parameters values, a = 9.6546 (4) Ǻ, b = 7.1637(3) Ǻ, c = 24.3606 (12) Ǻ, α = γ = 90°, β = 92.458(2)° and volume = 1683.29(13) Ǻ{sup 3}. The FT-IR and FT-Raman spectrum were used to affirm the functional group of the title compound. The chemical structure of 8HQ2C5N was scrutinized by {sup 13}C and {sup 1}H NMR spectral analysis and thermal stability through the differential scanning calorimetry study. Using optical studies the lower cut-off wavelength and optical band gap of 8HQ2C5N were found to be 364 nm and 3.17 eV respectively. Using the single oscillator model suggested by Wemple – Didomenico, the oscillator energy (E{sub o}), the dispersion energy (E{sub d}) and static dielectric constant (ε{sub o}) were estimated. The third-order susceptibility were determined as Im χ{sup (3)} = 2.51 × 10{sup −5} esu and Re χ{sup (3)} = 4.46 × 10{sup −7} esu. The theoretical third-order nonlinear optical susceptibility χ{sup (3)} was calculated and the results were compared with experimental value. Photoluminescence spectrum of 8HQ2C5N crystal showed the yellow emission. The crystal had the single shot laser damage threshold of 5.562 GW/cm{sup 2}. Microhardness measurement showed that 8HQ2C5N belongs to a soft material category. - Highlights: • A new organic single crystals were grown and the crystal structure was reported. • Crystal possess, good transmittance, thermal and mechanical stability. • Single shot LDT value is found to be

  12. Differential effects of miR-34c-3p and miR-34c-5p on SiHa cells proliferation apoptosis, migration and invasion

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, Jesus Adrian [Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, CINVESTAV, Av. IPN 2508, Mexico 07360 D.F. (Mexico); Alvarez-Salas, Luis Marat, E-mail: lalvarez@cinvestav.mx [Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, CINVESTAV, Av. IPN 2508, Mexico 07360 D.F. (Mexico)

    2011-06-10

    Highlights: {yields} In this study we examine miR-34c-3p and miR-34c-5p functions in SiHa cells. {yields} We study miRNA effect on cell proliferation, anchorage independent growth, apoptosis, cell motility and invasion. {yields} We find that miR-34c-3p and miR-34c-5p inhibition of proliferation and anchorage independent growth are exclusive to SiHa cells. {yields} miR-34c-3p induces apoptosis and inhibits cell motility and invasion in SiHa cells. {yields} In this study we conclude that miR-34c-3p functions as a tumor suppressor differ from miR-34c-5p. -- Abstract: MicroRNAs (miRNA) regulate expression of several genes associated with human cancer. Here, we analyzed the function of miR-34c, an effector of p53, in cervical carcinoma cells. Expression of either miR-34c-3p or miR-34c-5p mimics caused inhibition of cell proliferation in the HPV-containing SiHa cells but not in other cervical cells irrespective of tumorigenicity and HPV content. These results suggest that SiHa cells may lack of regulatory mechanisms for miR-34c. Monolayer proliferation results showed that miR-34c-3p produced a more pronounced inhibitory effect although both miRNAs caused inhibition of anchorage independent growth at similar extent. However, ectopic expression of pre-miR-34c-3p, but not pre-miR-34c-5p, caused S-phase arrest in SiHa cells triggering a strong dose-dependent apoptosis. A significant inhibition was observed only for miR-34c-3p on SiHa cells migration and invasion, therefore implying alternative regulatory pathways and targets. These results suggest differential tumor suppressor roles for miR-34c-3p and miR-34c-5p and provide new insights in the understanding of miRNA biology.

  13. Differential effects of miR-34c-3p and miR-34c-5p on SiHa cells proliferation apoptosis, migration and invasion

    International Nuclear Information System (INIS)

    Lopez, Jesus Adrian; Alvarez-Salas, Luis Marat

    2011-01-01

    Highlights: → In this study we examine miR-34c-3p and miR-34c-5p functions in SiHa cells. → We study miRNA effect on cell proliferation, anchorage independent growth, apoptosis, cell motility and invasion. → We find that miR-34c-3p and miR-34c-5p inhibition of proliferation and anchorage independent growth are exclusive to SiHa cells. → miR-34c-3p induces apoptosis and inhibits cell motility and invasion in SiHa cells. → In this study we conclude that miR-34c-3p functions as a tumor suppressor differ from miR-34c-5p. -- Abstract: MicroRNAs (miRNA) regulate expression of several genes associated with human cancer. Here, we analyzed the function of miR-34c, an effector of p53, in cervical carcinoma cells. Expression of either miR-34c-3p or miR-34c-5p mimics caused inhibition of cell proliferation in the HPV-containing SiHa cells but not in other cervical cells irrespective of tumorigenicity and HPV content. These results suggest that SiHa cells may lack of regulatory mechanisms for miR-34c. Monolayer proliferation results showed that miR-34c-3p produced a more pronounced inhibitory effect although both miRNAs caused inhibition of anchorage independent growth at similar extent. However, ectopic expression of pre-miR-34c-3p, but not pre-miR-34c-5p, caused S-phase arrest in SiHa cells triggering a strong dose-dependent apoptosis. A significant inhibition was observed only for miR-34c-3p on SiHa cells migration and invasion, therefore implying alternative regulatory pathways and targets. These results suggest differential tumor suppressor roles for miR-34c-3p and miR-34c-5p and provide new insights in the understanding of miRNA biology.

  14. Postvaccination C-Reactive Protein and C5/gp41732-744 Antibody Level Fold-Changes Over Baseline Are Independent Predictors of Therapeutic HIV Vaccine Effect in a Phase 2 Clinical Study of Vacc-4x.

    Science.gov (United States)

    Huang, Yunda; Zhang, Lily; Jolliffe, Darren; Sanchez, Brittany; Stjernholm, Grete; Jelmert, Øyvind; Ökvist, Mats; Sommerfelt, Maja A

    2018-03-01

    Therapeutic vaccination has the potential to contribute to functional HIV cure strategies. However, to show functional HIV cure, study participants must be taken off combination antiretroviral therapy (cART). The availability of suitable biomarkers that can predict viral load (VL) or CD4 count outcomes following therapeutic HIV vaccination would reduce the risks associated with cART interruption in such studies. This report sought to determine baseline and postvaccination biomarker predictors of vaccine effect (VE) on VL and CD4 counts following cART interruption in a double-blind, randomized phase 2 study of the peptide-based therapeutic HIV vaccine, Vacc-4x (n = 93), versus placebo (n = 43). Antibody responses to a novel envelope glycoprotein antigen, C5/gp41 732-744 , and three safety marker measurements [C-reactive protein (CRP), white blood cell, and lactate dehydrogenase] were considered. Interaction tests in univariate and multivariate linear regression models were used to estimate the effect of biomarkers on VE, defined as the VL or CD4 count difference in Vacc-4x versus placebo groups. The reported q-values (considered significant for hypothesis-generating purposes if ≤0.2) accounted for multiple comparisons using the false discovery rate method. Data were analyzed from all available 58 Vacc-4x and 25 placebo recipients before cART resumption. Lower postvaccination fold-change over baseline of CRP concentration (interaction p- (q-) value = 0.005 (0.11) for VL) and higher fold-change of anti-C5/gp41 732-744 antibody levels (0.005 (0.11) for VL and 0.009 (0.20) for CD4) were associated with Vacc-4x benefit. These findings suggest potential roles for inflammation and immune activation markers in predicting therapeutic HIV VE.

  15. Biomass pre-treatment for co-production of high-concentration C5- and C6-carbohydrates and their derivatives

    Science.gov (United States)

    Dumesic, James A.; Martin Alonso, David; Luterbacher, Jeremy Scott

    2016-06-07

    Described is a method of processing biomass to separate it into a liquid fraction enriched in solubilized C5-sugar-containing oligomers and C-5 sugar monomers and a solid fraction enriched in substantially insoluble cellulose and C6-sugar-containing oligomers. The method includes the steps of reacting biomass with a solvent system comprising water, at least one lactone, or at least one furan, or at least one cyclic ether, and at least one acid, for a time and at a temperature to yield the liquid and solid fractions. The liquid and solid fractions may then be separated. Gamma-valeroloactone is a preferred lactone for use in the solvent system. Tetrahydrofuran is a preferred furan species for use in the solvent system.

  16. IFN-γ-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils.

    Science.gov (United States)

    Kim, Ji Hyung; Oh, Sae Jin; Ahn, Sehee; Chung, Doo Hyun

    2014-07-01

    A role for NKT cells has been implicated in sepsis, but the mechanism by which NKT cells contribute to sepsis remains unclear. Here, we examined WT and NKT-cell-deficient mice of C57BL/6 background during cecal ligation and puncture-induced sepsis. The levels of C5a, IFN-γ, and IL-10 were higher in the serum and peritoneal fluid of WT mice than in those of CD1d(-/-) mice, while the mortality rate was lower in CD1d(-/-) mice than in WT mice. C5a blockade decreased mortality of WT mice during sepsis, whereas it did not alter that of CD1d(-/-) mice. As assessed by intracellular staining, NKT cells expressed IFN-γ, while neutrophils expressed IL-10. Upon coculture, IL-10-deficient NKT cells enhanced IL-10 production by WT, but not IFN-γR-deficient, neutrophils. Meanwhile, CD1d(-/-) mice exhibited high CD55 expression on neutrophils during sepsis, whereas those cells from WT mice expressed minimal levels of CD55. Recombinant IL-10 administration into CD1d(-/-) mice reduced CD55 expression on neutrophils. Furthermore, adoptive transfer of sorted WT, but not IFN-γ-deficient, NKT cells into CD1d(-/-) mice suppressed CD55 expression on neutrophils, but increased IL-10 and C5a levels. Taken together, IFN-γ-producing NKT cells enhance C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils, thereby exacerbating sepsis. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Validation of PROBA-V GEOV1 and MODIS C5 & C6 fAPAR Products in a Deciduous Beech Forest Site in Italy

    Czech Academy of Sciences Publication Activity Database

    Nestola, E.; Sanchez-Zapero, J.; Latorre, C.; Mazzenga, F.; Matteucci, G.; Calfapietra, Carlo; Camacho, F.

    2017-01-01

    Roč. 9, č. 2 (2017), č. článku 126. ISSN 2072-4292 Institutional support: RVO:86652079 Keywords : fAPAR * validation * PROBA-V GEOV1 * MODIS C5 * MODIS C6 * beech forest * up-scaling * GCOS requirements * in-situ comparison and evaluation Subject RIV: EH - Ecology, Behaviour OBOR OECD: Environmental sciences (social aspects to be 5.7) Impact factor: 3.244, year: 2016

  18. New technique targeting the C5 nerve root proximal to the traditional interscalene sonoanatomical approach is analgesic for outpatient arthroscopic shoulder surgery.

    Science.gov (United States)

    Dobie, Katherine H; Shi, Yaping; Shotwell, Matthew S; Sandberg, Warren S

    2016-11-01

    Regional anesthesia and analgesia for shoulder surgery is most commonly performed via interscalene nerve block. We developed an ultrasound-guided technique that specifically targets the C5 nerve root proximal to the traditional interscalene block and assessed its efficacy for shoulder analgesia. Prospective case series. Vanderbilt Bone and Joint Surgery Center. Patients undergoing shoulder arthroscopy at an ambulatory surgery center. Thirty-five outpatient shoulder arthroscopy patients underwent an analgesic nerve block using a new technique where ultrasound visualization of the C5 nerve root served as the primary target at a level proximal to the traditional interscalene approach. The block was performed with 15mL of 0.5% plain ropivicaine. Post anesthesia care unit pain scores, opioid consumption, hand strength, and duration of block were recorded. Cadaver dissection after injection with methylene blue confirmed that the primary target under ultrasound visualization was the C5 nerve root. Pain scores revealed 97% patients had 0/10 pain at arrival to PACU, with 91% having a pain score of 3/10 or less at discharge from PACU. Medical Research Council (MRC) hand strength mean (SD) score was 4.17 (0.92) on a scale of 1-5. The mean (SD) duration of the block was 13.9 (3.5) hours. A new technique for ultrasound-guided blockade at the level of the C5 nerve root proximal to the level of the traditional interscalene block is efficacious for shoulder post-operative pain control. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Effects of C5/C6 Intervertebral Space Distraction Height on Pressure on the Adjacent Intervertebral Disks and Articular Processes and Cervical Vertebrae Range of Motion.

    Science.gov (United States)

    Lu, Tingsheng; Luo, Chunshan; Ouyang, Beiping; Chen, Qiling; Deng, Zhongliang

    2018-04-25

    BACKGROUND This study aimed to investigate the association between range of motion of the cervical vertebrae and various C5/C6 intervertebral space distraction heights. MATERIAL AND METHODS The cervical vertebrae from 6 fresh adult human cadavers were used to prepare the models. Changes in C4/C5 and C6/C7 intervertebral disk pressures, articular process pressure, and range of motion of the cervical vertebrae before and after the distraction of the C5/C6 intervertebral space at benchmark heights of 100%, 120%, 140%, and 160% were tested under different exercise loads. RESULTS The pressure on the adjacent intervertebral disks was highest with the standing upright position before distraction, varied with different positions of the specimens and distraction heights after distraction, and was closest to that before distraction at a distraction height of 120% (Particular processes was highest with left and right rotations before distraction, varied with different positions of the specimens and distraction heights after distraction, and was lowest under the same exercise load with different positions at a distraction height of 120% (Pdistraction and at a distraction height of 120% after distraction, respectively (Particular processes and range of motion of the cervical vertebrae and is therefore an appropriate intervertebral space distraction height.

  20. Optimizing C4+ and C5+ beams of the Kei2 electron cyclotron resonance ion source using a special gas-mixing technique

    International Nuclear Information System (INIS)

    Drentje, A.G.; Muramatsu, M.; Kitagawa, A.

    2006-01-01

    With the prototype electron cyclotron resonance ion source for the next carbon therapy facility in Japan a series of measurements has been performed in order (a) to find the best condition for producing high beam currents of C 4+ ions, and (b) to study the effect of 'special' gas mixing by using a chemical compound as a feed gas. The effect would then appear as an increase in high charge state production in this case of C 5+ ions. In 'regular' gas-mixing experiments it is well known that an isotopic phenomenon occurs: a heavier isotope of the mixing gas is increasing the production of high charge states of the beam gas ions. A similar isotopic effect has been found in the present experiment: with deuterated methane (CD 4 gas) the C 5+ beam currents are about 10% higher than with regular methane (CH 4 gas). The 'mixing-gas' ratio D (or H) to C can be decreased by choosing, e.g., butane gas; in this case the isotopic effect for C 5+ production is even stronger (>15%). For production of C 4+ ions the isotopic effect appears to be absent. Clearly this is related to the much easier production. It turns out that the relative amount of carbon is much more important: butane gives about 10% higher C 4+ -ion currents than methane

  1. Predictable topography simulation of SiO2 etching by C5F8 gas combined with a plasma simulation, sheath model and chemical reaction model

    International Nuclear Information System (INIS)

    Takagi, S; Onoue, S; Iyanagi, K; Nishitani, K; Shinmura, T; Kanoh, M; Itoh, H; Shioyama, Y; Akiyama, T; Kishigami, D

    2003-01-01

    We have developed a simulation for predicting reactive ion etching (RIE) topography, which is a combination of plasma simulation, the gas reaction model, the sheath model and the surface reaction model. The simulation is applied to the SiO 2 etching process of a high-aspect-ratio contact hole using C 5 F 8 gas. A capacitively coupled plasma (CCP) reactor of an 8-in. wafer was used in the etching experiments. The baseline conditions are RF power of 1500 W and gas pressure of 4.0 Pa in a gas mixture of Ar, O 2 and C 5 F 8 . The plasma simulation reproduces the tendency that CF 2 radical density increases rapidly and the electron density decreases gradually with increasing gas flow rate of C 5 F 8 . In the RIE topography simulation, the etching profiles such as bowing and taper shape at the bottom are reproduced in deep holes with aspect ratios greater than 19. Moreover, the etching profile, the dependence of the etch depth on the etching time, and the bottom diameter can be predicted by this simulation

  2. An organic solvent-, detergent-, and thermo-stable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus licheniformis 3C5.

    Science.gov (United States)

    Rachadech, W; Navacharoen, A; Ruangsit, W; Pongtharangkul, T; Vangnai, A S

    2010-01-01

    Bacillus licheniformis 3C5, isolated as mesophilic bacterium, exhibited tolerance towards a wide range of non-polar and polar organic solvents at 45 degrees C. It produced an extracellular organic solvent-stable protease with an apparent molecular mass of approximately 32 kDa. The inhibitory effect of PMSF and EDTA suggested it is likely to be an alkaline serine protease. The protease was active over abroad range of temperatures (45-70 degrees C) and pH (8-10) range with an optimum activity at pH 10 and 65 degrees C. It was comparatively stable in the presence ofa relatively high concentration (35% (v/v)) of organic solvents and various types of detergents even at a relatively high temperature (45 degrees C). The protease production by B. licheniformis 3C5 was growth-dependent. The optimization of carbon and nitrogen sources for cell growth and protease production revealed that yeast extract was an important medium component to support both cell growth and the protease production. The overall properties of the protease produced by B. licheniformis 3C5 suggested that this thermo-stable, solvent-stable, detergent-stable alkaline protease is a promising potential biocatalyst for industrial and environmental applications.

  3. Bayesian Safety Risk Modeling of Human-Flightdeck Automation Interaction

    Science.gov (United States)

    Ancel, Ersin; Shih, Ann T.

    2015-01-01

    Usage of automatic systems in airliners has increased fuel efficiency, added extra capabilities, enhanced safety and reliability, as well as provide improved passenger comfort since its introduction in the late 80's. However, original automation benefits, including reduced flight crew workload, human errors or training requirements, were not achieved as originally expected. Instead, automation introduced new failure modes, redistributed, and sometimes increased workload, brought in new cognitive and attention demands, and increased training requirements. Modern airliners have numerous flight modes, providing more flexibility (and inherently more complexity) to the flight crew. However, the price to pay for the increased flexibility is the need for increased mode awareness, as well as the need to supervise, understand, and predict automated system behavior. Also, over-reliance on automation is linked to manual flight skill degradation and complacency in commercial pilots. As a result, recent accidents involving human errors are often caused by the interactions between humans and the automated systems (e.g., the breakdown in man-machine coordination), deteriorated manual flying skills, and/or loss of situational awareness due to heavy dependence on automated systems. This paper describes the development of the increased complexity and reliance on automation baseline model, named FLAP for FLightdeck Automation Problems. The model development process starts with a comprehensive literature review followed by the construction of a framework comprised of high-level causal factors leading to an automation-related flight anomaly. The framework was then converted into a Bayesian Belief Network (BBN) using the Hugin Software v7.8. The effects of automation on flight crew are incorporated into the model, including flight skill degradation, increased cognitive demand and training requirements along with their interactions. Besides flight crew deficiencies, automation system

  4. Synthesis and structural characterization of PHP[(C(5)Me(4))(2)], a monodentate chiral phosphine derived from intramolecular C-C coupling of tetramethylcyclopentadienyl groups: an evaluation of steric and electronic properties.

    Science.gov (United States)

    Shin, J H; Bridgewater, B M; Churchill, D G; Parkin, G

    2001-10-22

    The chiral monodentate phosphine PhP[(C(5)Me(4))(2)] is readily obtained by oxidation of the lithium complex Li(2)[PhP(C(5)Me(4))(2)] with I(2), which couples the two cyclopentadienyl groups to form a five-membered heterocyclic ring. The steric and electronic properties of PhP[(C(5)Me(4))(2)] have been evaluated by X-ray diffraction and IR spectroscopic studies on a variety of derivatives, including Ph[(C(5)Me(4))(2)]PE (E = S, Se), Cp*MCl(4)[P[(C(5)Me(4))(2)]Ph] (M = Mo, Ta), Ir[P[(C(5)Me(4))(2)]Ph](2)(CO)Cl, and CpFe(CO)[PhP[(C(5)Me(4))(2)

  5. Atmospheric histories and growth trends of C4F10, C5F12, C6F14, C7F16 and C8F18

    Directory of Open Access Journals (Sweden)

    R. F. Weiss

    2012-05-01

    Full Text Available Atmospheric observations and trends are presented for the high molecular weight perfluorocarbons (PFCs: decafluorobutane (C4F10, dodecafluoropentane (C5F12, tetradecafluorohexane (C6F14, hexadecafluoroheptane (C7F16 and octadecafluorooctane (C8F18. Their atmospheric histories are based on measurements of 36 Northern Hemisphere and 46 Southern Hemisphere archived air samples collected between 1973 to 2011 using the Advanced Global Atmospheric Gases Experiment (AGAGE "Medusa" preconcentration gas chromatography-mass spectrometry systems. A new calibration scale was prepared for each PFC, with estimated accuracies of 6.8% for C4F10, 7.8% for C5F12, 4.0% for C6F14, 6.6% for C7F16 and 7.9% for C8F18. Based on our observations the 2011 globally averaged dry air mole fractions of these heavy PFCs are: 0.17 parts-per-trillion (ppt, i.e., parts per 1012 for C4F10, 0.12 ppt for C5F12, 0.27 ppt for C6F14, 0.12 ppt for C7F16 and 0.09 ppt for C8F18. These atmospheric mole fractions combine to contribute to a global average radiative forcing of 0.35 mW m−2, which is 6% of the total anthropogenic PFC radiative forcing (Montzka and Reimann, 2011; Oram et al., 2012. The growth rates of the heavy perfluorocarbons were largest in the late 1990s peaking at 6.2 parts per quadrillion (ppq, i.e., parts per 1015 per year (yr for C4F10, at 5.0 ppq yr−1 for C5F12 and 16.6 ppq yr−1 for C6F14 and in the early 1990s for C7F16 at 4.7 ppq yr−1 and in the mid 1990s for C8F18 at 4.8 ppq yr−1. The 2011 globally averaged mean atmospheric growth rates of these PFCs are subsequently lower at 2.2 ppq yr−1 for C4F10, 1.4 ppq yr−1 for C5F12, 5.0 ppq yr−1 for C6F14, 3.4 ppq yr−1 for C7F16 and 0.9 ppq yr−1 for C8F18. The more recent slowdown in the growth rates suggests that emissions are declining as compared to the 1980s and 1990s.

  6. Synthesis and Electronic Structure of Dissymmetrical, Naphthalene-Bridged Sandwich Complexes [Cp ' Fe(mu-C10H8)MCp*](x) (x=0,+1; M = Fe, Ru; Cp ' = eta(5)-C5H2-1,2,4-tBu(3); Cp* = eta(5)-C5Me5)

    NARCIS (Netherlands)

    Malberg, J.; Lupton, E.; Schnöckelborg, E.M.; de Bruin, B.; de Sutter, J.; Meyer, K.; Hartl, F.; Wolf, R.

    2013-01-01

    The dissymmetrical naphthalene-bridged complexes [Cp'Fe(mu-C10H8)FeCp*] (3; Cp* = eta(5)-C5Me5, Cp' = eta(5)-C5H2-1,2,4-tBu(3)) and [Cp'Fe(mu-C10H8)RuCp*] (4) were synthesized via a one-pot procedure from FeCl2(thf)(1.5), Cp'K, KC10H8, and [Cp*FeCl(tmeda)] (tmeda =

  7. Reaction of ether and thioether functionalised 1-alkenes with the cationic permethylzirconocene olefin polymerisation catalyst [(eta(5)-C5Me5)(2)ZrMe](+). Molecular structure of the insertion product [(eta(5)-C5Me5)(2)ZrCH2CH(Me)CH2OEt](+)

    NARCIS (Netherlands)

    Bijpost, Erik A; Zuideveld, Martin A.; Meetsma, Auke; Teuben, Jan H

    1998-01-01

    Reaction of [(η5-C5Me5)2ZrMe][MeB(C6F5)3] (1) with (thio)ether functionalised alkenes: 3-ethoxy-1-propene and 3-(methylthio)-1-propene gives stable insertion products [(η5-C5Me5)2ZrCH2CH(Me)CH2XR][MeB(C6F5)3] (2: X = O, R = Et; 3: X = S, R = Me) in which the (thio)ether function is intramolecularly

  8. A Novel simultaneous-Saccharification-Fermentation Strategy for Efficient Co-fermentation of C5 and C6 Sugars Using Native, Non-GMO Yeasts

    Energy Technology Data Exchange (ETDEWEB)

    Varanasi, Sasidhar [Univ. of Toledo, OH (United States); Relue, Patricia [Univ. of Toledo, OH (United States)

    2013-09-30

    Economic bioethanol production is critically dependent upon the ability to convert both the hexose (C6) and pentose (C5) sugars resulting from cellulose and hemicellulose. C5 sugars are not readily fermentable by native Saccharomyces cerevisiae. Genetically Modified Organisms (GMOs) are designed to ferment xylose, but their stability, ethanol yield, environmental impact, and survival under conditions of industrial fermentation are unproven. In this project, we developed a novel approach for efficient fermentation of both C5 and C6 sugars using native S. Cerevisiae by exploiting its ability to produce ethanol from xylulose - the keto-isomer of xylose. While the isomerization of xylose to xylulose can be accomplished via commercially (and cheaply) available Xylose Isomerase (XI) (Sweetzyme™), this conversion has an extremely unfavorable equilibrium (xylose:xylose is about 5:1). To address this, we developed two alternate strategies. In the first, the two enzymes XI and urease are coimmobilized on solid support particles to enable complete isomerization of xylose to xylulose under pH conditions suitable for fermentation, in a simultaneous-isomerization-fermentation (SIF) mode. The ability of our technology to conduct isomerization of xylose under pH conditions suitable for both saccharification and fermentation opens the possibility of SSF with native yeasts for the first time. Herein, we performed specific research tasks for implementation of our technology in several modes of operation, including simultaneous-isomerization-and-fermentation (SIF), simultaneous-saccharification-and-isomerization (SSI) followed by fermentation, and SSF mode with the biomass feedstock poplar. The projected economics of our process are very favorable in comparison to the costs associated with engineering, licensing and propagating GMOs. This novel fermentation technology is readily accessible to rural farming economies for implementation in cellulosic ethanol production facilities.

  9. Quadriplegia recovery after hemi-section and transplant model of spinal cord at the level of C5 and C6.

    Science.gov (United States)

    Bitar-Alatorre, W E; Segura-Torres, J E; Rosales-Corral, S A; Jiménez-Avila, J M; Huerta-Viera, M

    2011-03-10

    A spinal cord hemi-section with a homologous transplant of medullar tissue at the level of C5-C6 and preservation of the anterior spinal artery was used to evaluate the histological characteristics such as quantity and quality of axons, myelin index and blood vessels after quadriplegia recovery. Vascular changes after spinal injury results in severe endothelial damage, axonal edema, neuronal necrosis and demyelinization as well as cysts and infarction. Preservation of the anterior spinal artery has demonstrated clinical recuperation; therefore, in addition to the lesion we included a homologous transplant to visualize changes at a cellular level. Two groups of dogs (hemi-section and transplant) went through a traumatic spinal cord hemi-section of 50% at the level of C5-C6. The transplant group formed by animals which simultaneously had 4 mm of spinal cord removed and the equal amount substituted from a donor animal at the level of C5-C6 corresponding to the half right side; both preserving the anterior spinal artery. Histological evaluation of all groups took place at days 3 (acute) and 28 (chronic) post-operation. Changes of degeneration and axonal regeneration were found in the hemi-section and transplant groups at acute and chronic time, as well as same quadriplegia recovery at chronic time in the hemi-section and transplant groups which closely related to mechanisms which participate in regeneration and functional recuperation due to the preservation of the anterior spinal artery and presence of new blood vessels. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  10. Synthesis, Characterization and In Vitro Anticancer Activity of C-5 Curcumin Analogues with Potential to Inhibit TNF-α-Induced NF-κB Activation

    Directory of Open Access Journals (Sweden)

    Amit Anthwal

    2014-01-01

    Full Text Available In a search of new compounds active against cancer, synthesis of a series of C-5 curcumin analogues was carried out. The new compounds demonstrated good cytotoxicity against chronic myeloid leukemia (KBM5 and colon cancer (HCT116 cell lines. Further, these compounds were found to have better potential to inhibit TNF-α-induced NF-κB activation in comparison to curcumin, which show their potential to act as anti-inflammatory agents. Some compounds were found to show higher cytotoxicity against cancer cell lines in comparison to curcumin used as standard.

  11. CFH Y402H polymorphism and the complement activation product C5a: effects on NF-κB activation and inflammasome gene regulation.

    Science.gov (United States)

    Cao, Sijia; Wang, Jay Ching Chieh; Gao, Jiangyuan; Wong, Matthew; To, Elliott; White, Valerie A; Cui, Jing Z; Matsubara, Joanne A

    2016-05-01

    The Y402H polymorphism in the complement factor H (CFH) gene is an important risk factor for age-related macular degeneration (AMD). Complement activation products and proinflammatory cytokines are associated with this polymorphism at the systemic level, but less is known of the associations in the outer retina of the genotyped eye. Here we investigate complement activation products and their role in nuclear factor (NF)-κB activation and gene expression of the NLRP3 inflammasome pathway. Postmortem donor eyes were genotyped for the CFH Y402H polymorphism and assessed for complement C3a, C5a, interleukin (IL)-18 and tumour necrosis factor (TNF)-α. ARPE19 cells were stimulated basolaterally with C5a or TNF-α in polarised cultures. NF-κB activation was assessed with a reporter cell line. Gene expression of inflammasome-related (NLRP3, caspase-1, IL-1β and IL-18) and classic inflammatory (IL-6 and IL-8) genes was studied. The distribution of inflammasome products, IL-1β and IL-18, was studied in postmortem donor eyes with AMD pathologies. Eyes with the homozygous at-risk variant demonstrated higher levels of C5a, IL-18 and TNF-α in Bruch's membrane and choroid. C5a promoted NF-κB activation and upregulation of IL-18 in polarised ARPE19. TNF-α promoted NF-κB activation and gene expression of caspase-1, IL-1β, IL-18, IL-6 and IL-8, but downregulated NLRP3. In eyes with geographic atrophy, strong immunoreactivity was observed for inflammasome products IL-1β and IL-18 compared with age-matched controls. The at-risk polymorphism of the CFH Y402H may contribute to AMD disease process through increased complement and NF-κB activation, and the upregulation of IL-18, a product of inflammasome activation. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  12. Immunoscintigraphy of adenocarcinomas by means of 111In-labelled F(ab')2 fragments of anti-CEA monoclonal antibody F023C5

    International Nuclear Information System (INIS)

    Riva, P.; Paganelli, G.; Callegaro, L.

    1988-01-01

    F(ab') 2 fragments of F023C5, an anti-CEA monoclonal antibody, were conjugated to diethylenetriamine pentaacetic acid (DTPA) and converted into a ready to use reagent for instant 111 In-labelling. The resulting 111 In radiopharmaceutical was administered intravenously and tested for its ability to image (at 48-72 h after administration) 31 primary and 85 metastatic carcinoma lesions in 70 adenocarcinoma patients (26 gastrointestinal, 18 breast and 26 lung tumour patients) whose serum CEA was elevated in 43 cases and normal in the other 27. (author)

  13. Cloning and Expression of Three New Azotobacter vinelandii Genes Closely Related to a Previously Described Gene Family Encoding Mannuronan C-5-Epimerases

    OpenAIRE

    Svanem, Britt Iren Glærum; Skjåk-Bræk, Gudmund; Ertesvåg, Helga; Valla, Svein

    1999-01-01

    The cloning and expression of a family of five modular-type mannuronan C-5-epimerase genes from Azotobacter vinelandii (algE1 to -5) has previously been reported. The corresponding proteins catalyze the Ca2+-dependent polymer-level epimerization of β-d-mannuronic acid to α-l-guluronic acid (G) in the commercially important polysaccharide alginate. Here we report the identification of three additional structurally similar genes, designated algE6, algE7, and algY. All three genes were sequenced...

  14. Plasmin cleaves fibrinogen and the human complement proteins C3b and C5 in the presence of Leptospira interrogans proteins: A new role of LigA and LigB in invasion and complement immune evasion.

    Science.gov (United States)

    Castiblanco-Valencia, Mónica Marcela; Fraga, Tatiana Rodrigues; Pagotto, Ana Helena; Serrano, Solange Maria de Toledo; Abreu, Patricia Antonia Estima; Barbosa, Angela Silva; Isaac, Lourdes

    2016-05-01

    Plasminogen is a single-chain glycoprotein found in human plasma as the inactive precursor of plasmin. When converted to proteolytically active plasmin, plasmin(ogen) regulates both complement and coagulation cascades, thus representing an important target for pathogenic microorganisms. Leptospira interrogans binds plasminogen, which is converted to active plasmin. Leptospiral immunoglobulin-like (Lig) proteins are surface exposed molecules that interact with extracellular matrix components and complement regulators, including proteins of the FH family and C4BP. In this work, we demonstrate that these multifunctional molecules also bind plasminogen through both N- and C-terminal domains. These interactions are dependent on lysine residues and are affected by ionic strength. Competition assays suggest that plasminogen does not share binding sites with C4BP or FH on Lig proteins at physiological molar ratios. Plasminogen bound to Lig proteins is converted to proteolytic active plasmin in the presence of urokinase-type plasminogen activator (uPA). Lig-bound plasmin is able to cleave the physiological substrates fibrinogen and the complement proteins C3b and C5. Taken together, our data point to a new role of LigA and LigB in leptospiral invasion and complement immune evasion. Plasmin(ogen) acquisition by these versatile proteins may contribute to Leptospira infection, favoring bacterial survival and dissemination inside the host. Copyright © 2016. Published by Elsevier GmbH.

  15. Utilisation of an eta(3)-allyl hydride complex, formed by UV irradiation, as a controlled source of 16-electron (eta(5)-C(5)Me(5))Rh(CH(2)[double bond, length as m-dash]CHMe).

    Science.gov (United States)

    Sexton, Catherine J; López-Serrano, Joaquín; Lledós, Agustí; Duckett, Simon B

    2008-10-21

    Low temperature UV irradiation of solutions of (eta(5)-C(5)Me(5))Rh(CH(2)[double bond, length as m-dash]CHMe)(2) yields (eta(5)-C(5)Me(5))Rh(eta(3)-CH(2)CHCH(2))(H), which provides controlled access to the 16-electron fragment (eta(5)-C(5)Me(5))Rh(CH(2)[double bond, length as m-dash]CHMe).

  16. Cocaine-associated retiform purpura: a C5b-9-mediated microangiopathy syndrome associated with enhanced apoptosis and high levels of intercellular adhesion molecule-1 expression.

    Science.gov (United States)

    Magro, Cynthia M; Wang, Xuan

    2013-10-01

    Cocaine-associated retiform purpura is a recently described entity characterized by striking hemorrhagic necrosis involving areas of skin associated with administration of cocaine. Levamisole, an adulterant in cocaine, has been suggested as the main culprit pathogenetically. Four cases of cocaine-associated retiform purpura were encountered in the dermatopathology practice of C. M. Magro. The light microscopic findings were correlated with immunohistochemical and immunofluorescence studies. All 4 cases showed a very striking thrombotic diathesis associated with intravascular macrophage accumulation. Necrotizing vasculitis was noted in 1 case. Striking intercellular adhesion molecule-1 (ICAM-1)/CD54 expression in vessel wall along with endothelial expression of caspase 3 and extensive vascular C5b-9 deposition was observed in all biopsies examined. Cocaine-induced retiform purpura is a C5b-9-mediated microvascular injury associated with enhanced apoptosis and prominent vascular expression of ICAM-1, all of which have been shown in prior in vitro and in vivo murine models to be a direct effect of cocaine metabolic products. Antineutrophilic cytoplasmic antibody and antiphospholipid antibodies are likely the direct sequelae of the proapoptotic microenvironment. The inflammatory vasculitic lesion could reflect the downstream end point reflective of enhanced ICAM-1 expression and the development of antineutrophilic cytoplasmic antibody. Levamisole likely works synergistically with cocaine in the propagation of this syndromic complex.

  17. Studies on the O-specific polysaccharide of the lipopolysaccharide from the Pseudomonas mediterranea strain C5P1rad1, a bacterium pathogenic of tomato and chrysanthemum.

    Science.gov (United States)

    Zdorovenko, Evelina L; Cimmino, Alessio; Marchi, Guido; Shashkov, Alexander S; Fiori, Mario; Knirel, Yuriy A; Evidente, Antonio

    2017-08-07

    An O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas mediterranea strain C5P1rad1, the causal agents of tomato pith necrosis and Chrysanthemum stem rot, and studied by one- and two-dimensional 1 H and 13 C NMR spectroscopy. The following structure of the trisaccharide repeating unit of the OPS was established, which, to our knowledge, is unique among the known bacterial polysaccharide structures: →4)-β-d-ManpNAc3NAcA-(1 → 4)-β-d-ManpNAc3NAcA-(1 → 3)-α-d-QuipNAc4NAc-(1→ where QuiNAc4NAc and ManNAc3NAcA indicate 2,4-diacetamido-2,4,6-trideoxyglucose and 2,3-diacetamido-2,3-dideoxymannuronic acid, respectively. Pre-treatment of leaves with LPS or OPS preparations at 250 and 50 μg mL -1 did not inhibit development of a hypersensitivity reaction induced by P. mediterranea C5P1rad1 on tobacco, tomato and chrysanthemum plants. The same preparations at 250 μg mL -1 partially prevented elicitation of the hypersensitivity reaction by Pseudomonas syringae KVPT7RC on chrysanthemum but not tobacco and tomato. Copyright © 2017. Published by Elsevier Ltd.

  18. Application of backflush and micro-flow techniques to the analysis of C5-C13 hydrocarbons in crude oils and its geochemical significance

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    An Agilent 6890N GC equipped with both FID and Agilent 5975 MSD analyzer has been employed to analyze C5-C13 hydrocarbon fractions of crude oils. A technical combination of program temperature volatilizer injection, backflush and micro-flow controller afforded a fine separation of C5-C13 compounds on a PONA column with the heavy part of crude oils being cut off before entering the analytical column. Both GC-FID chromatogram and GC-MS mass chromatograrns (MID and full scan) could be obtained at the same time. The retension time differences of nC6-nC13 alkanes between GC and TIC were in the range of 0.02-0. 58 minutes. Totally 286 peaks have been assigned group compositions. Results on 8 typical oil samples from CNPC Key Laboratory Crude Oil Library showed that the characteristics of C6-C13 hydrocarbon group composition could be used in oil-oil correlation studies.

  19. Structural, bonding, anisotropic mechanical and thermal properties of Al4SiC4 and Al4Si2C5 by first-principles investigations

    Directory of Open Access Journals (Sweden)

    Liang Sun

    2016-09-01

    Full Text Available The structural, bonding, electronic, mechanical and thermal properties of ternary aluminum silicon carbides Al4SiC4 and Al4Si2C5 are investigated by first-principles calculations combined with the Debye quasi-harmonic approximation. All the calculated mechanical constants like bulk, shear and Young's modulus are in good agreement with experimental values. Both compounds show distinct anisotropic elastic properties along different crystalline directions, and the intrinsic brittleness of both compounds is also confirmed. The elastic anisotropy of both aluminum silicon carbides originates from their bonding structures. The calculated band gap is obtained as 1.12 and 1.04 eV for Al4SiC4 and Al4Si2C5 respectively. From the total electron density distribution map, the obvious covalent bonds exist between Al and C atoms. A distinct electron density deficiency sits between AlC bond along c axis among Al4SiC4, which leads to its limited tensile strength. Meanwhile, the anisotropy of acoustic velocities for both compounds is also calculated and discussed.

  20. Excretion of complement proteins and its activation marker C5b-9 in IgA nephropathy in relation to renal function

    Directory of Open Access Journals (Sweden)

    Onda Kisara

    2011-11-01

    Full Text Available Abstract Background Glomerular damage in IgA nephropathy (IgAN is mediated by complement activation via the alternative and lectin pathways. Therefore, we focused on molecules stabilizing and regulating the alternative pathway C3 convertase in urine which might be associated with IgAN pathogenesis. Methods Membrane attack complex (MAC, properdin (P, factor H (fH and Complement receptor type 1 (CR1 were quantified in urine samples from 71 patients with IgAN and 72 healthy controls. Glomerular deposition of C5, fH and P was assessed using an immunofluorescence technique and correlated with histological severity of IgAN and clinical parameters. Fibrotic changes and glomerular sclerosis were evaluated in renal biopsy specimens. Results Immunofluorescence studies revealed glomerular depositions of C5, fH and P in patients with IgAN. Urinary MAC, fH and P levels in IgAN patients were significantly higher than those in healthy controls (p Conclusions Complement activation occurs in the urinary space in IgAN and the measurement of levels of MAC and fH in the urine could be a useful indicator of renal injury in patients with IgAN.

  1. Influência da mono-hidrogenação da fração C5 na síntese do TAME usando Amberlyst 15 como catalisador Influence of mono-hydrogenation of C5 cut on the synthesis of TAME using Amberlyst 15

    Directory of Open Access Journals (Sweden)

    Gilberto da Cunha Gonçalves

    2001-05-01

    Full Text Available No presente trabalho, foi estudada a síntese do TAME, em fase líquida, a partir de uma fração C5 e metanol, utilizando Amberlyst 15 como catalisador. A fração C5 utilizada possui uma diolefina, que tem uma alta tendência à formação de gomas e um alto teor de mono-olefinas. Deste modo, o objetivo deste trabalho foi avaliar o efeito da mono-hidrogenação da fração C5 na síntese do TAME. A hidrogenação foi feita a 80oC e 10 kgf/cm2 de H2, usando um catalisador 0,3% Pt/Al2O3. A diolefina foi totalmente hidrogenada e o teor de mono-olefinas significativamente reduzido sem um decréscimo apreciável no teor de isoamilenos reativos. A eterificação foi feita em um reator tipo batelada de 250 mL, com agitação magnética. A temperatura foi variada na faixa de 50 a 90oC, com uma pressão de 10 kgf/cm2 e razão molar isoamileno/metanol de aproximadamente um. Os produtos de reação foram analisados por cromatografia gasosa. A conversão total observada foi aproximadamente a mesma para o os cortes original e mono-hidrogenado. Entretanto, os produtos obtidos utilizando a fração C5 original apresentaram cheiro forte e coloração, mesmos para os testes a temperaturas mais baixas, que são características da formação de gomas. Por outro lado, quando a fração mono-hidrogenada foi usada na eterificação, o cheiro forte e coloração não foram observados. Portanto, a mono-hidrogenação da fração C5 contendo os isoamilenos reativos é necessária para que o TAME produzido na reação de eterificação possa ser adicionado à gasolinaTAME was synthesized in liquid phase from refinery C5 cut and methanol using Amberlyst 15 as catalyst. The C5 stream contains mainly mono-olefins and, in a less amount, a diolefin, which have a high gum formation tendency. The main purpose of this research was to evaluate the effect of mono-hydrogenation of C5 cut on TAME synthesis. The hydrogenation step was carried out at 80 oC and 10 kgf/cm2 of H2

  2. The Variations and Trends of MODIS C5 & C6 Products’ Errors in the Recent Decade over the Background and Urban Areas of North China

    Directory of Open Access Journals (Sweden)

    Qi Zhang

    2016-09-01

    Full Text Available With ten-year (2004–2013 ground-based observations of Beijing Forest (BJF and Beijing City (BJC sites in North China, we validated the high-quality MODerate resolution Imaging Spectroradiometer (MODIS Collection 5 (C5 and Collection 6 (C6 Aerosol Optical Depth (AOD products’ precision and discussed the sensors degradation issues. The annual mean AOD and Angstrom exponent (α were 0.20 ± 0.02 and 0.83 ± 0.15 in the background over the past ten years, and they were 0.59 ± 0.07 and 1.13 ± 0.08 in the urban, respectively. Ground-based AOD had both slightly declining trends, with variations of 0.023 and 0.057 over the past decade in the background and urban, respectively. There were large differences among the eight kinds of MODIS AOD products (Terra vs. Aqua, C5 vs. C6, DT (Deep Target vs. DB (Deep Blue, and DTDB in the background and urban areas, but all the products’ monthly errors had larger variations in the spring and summer, and smaller ones in the autumn and winter. In the background, more than 62% of DT matchups for C5 and C6 products were within NASA’s expected error (EE envelope. In the urban, 69%~72% of C6 DB retrievals were falling within EE envelope. The new dataset named C6 DTDB had better performance in the background, whereas it overestimated by 37%~41% in the urban caused by surface reflectivity estimation error. The range of monthly average error varied from −0.21 to 0.28 in the background and from −0.63 to 0.48 in the urban. From the background to the urban areas, the retrieval errors of Terra and Aqua had slightly increased by 0.0023~0.0158 and 0.0011~0.0124 per year, respectively, which implied that the two MODIS instruments had degraded slowly.

  3. Experimental and Theoretical Studies of the Factors Affecting the Cycloplatination of the Chiral Ferrocenylaldimine (SC-[(η5-C5H5Fe{(η5-C5H4–C(H=N–CH(Me(C6H5}

    Directory of Open Access Journals (Sweden)

    Concepción López

    2014-11-01

    Full Text Available The study of the reactivity of the enantiopure ferrocenyl Schiff base (SC-[FcCH=N–CH(Me(C6H5] (1 (Fc = (η5-C5H5Fe(η5-C5H4 with cis-[PtCl2(dmso2] under different experimental conditions is reported. Four different types of chiral Pt(II have been isolated and characterized. One of them is the enantiomerically pure trans-(SC-[Pt{κ1-N[FcCH=N–CH(Me(C6H5]}Cl2(dmso] (2a in which the imine acts as a neutral N-donor ligand; while the other three are the cycloplatinated complexes: [Pt{κ2-C,N [(C6H4–N=CHFc]}Cl(dmso] (7a and the two diastereomers {(Sp,SC and (Rp,SC} of [Pt{κ2-C,N[(η5-C5H3–CH=N–{CH(Me(C6H5}]Fe(η5-C5H5}Cl(dmso] (8a and 9a, respectively. Isomers 7a-9a, differ in the nature of the metallated carbon atom [CPh (in 7a or CFc (in 8a and 9a] or the planar chirality of the 1,2-disubstituted ferrocenyl unit (8a and 9a. Reactions of 7a–9a with PPh3 gave [Pt{κ2-C,N[(C6H4–N=CHFc]}Cl(PPh3] (in 7b and the diastereomers (Sp,SC and (Rp,SC of [Pt{κ2-C,N[(η5-C5H3–CH=N–{CH(Me(C6H5}] Fe(η5-C5H5}Cl(PPh3] (8b and 9b, respectively. Comparative studies of the electrochemical properties and cytotoxic activities on MCF7 and MDA-MB231 breast cancer cell lines of 2a and cycloplatinated complexes 7b-9b are also reported. Theoretical studies based on DFT calculations have also been carried out in order to rationalize the results obtained from the cycloplatination of 1, the stability of the Pt(II complexes and their electrochemical properties.

  4. Effects of 60 MeV C5+ ion irradiation on PmT-PVC and p-TSA doped PoT-PVC blends

    International Nuclear Information System (INIS)

    Lakshmi, G.B.V.S.; Siddiqui, Azher M.; Ali, Vazid; Kulriya, Pawan K.; Zulfequar, M.

    2008-01-01

    Poly(m-toluidine) (PmT) and Poly(o-toluidine) (PoT) have been synthesized from derivatives of aniline (m-toluidine), (o-toluidine) monomers by chemical oxidative polymerization method. After polymerization, PoT powder was doped with p-toluene sulphonic acid (p-TSA) and the polymer powders were blended with poly vinyl chloride (PVC) to achieve PmT and p-TSA doped PoT dispersed films. XRD, FTIR and UV-visible studies were carried out to get their structural changes and optical information. These blends were irradiated by 60 MeV C 5+ ions with different fluences. Post Irradiation XRD, FTIR and UV-visible spectroscopy were also performed on all films. On p-TSA doped PoT-PVC blends dc-conductivity measurements are also carried out before and after irradiation. The results show structural modifications which lead to changes in optical and electrical properties

  5. Dynamic Monte Carlo transient analysis for the Organization for Economic Co-operation and Development Nuclear Energy Agency (OECD/NEA) C5G7-TD benchmark

    Energy Technology Data Exchange (ETDEWEB)

    Shaukat, Nadeem; Ryu, Min; Shim, Hyung Jin [Seoul National University, Seoul (Korea, Republic of)

    2017-08-15

    With ever-advancing computer technology, the Monte Carlo (MC) neutron transport calculation is expanding its application area to nuclear reactor transient analysis. Dynamic MC (DMC) neutron tracking for transient analysis requires efficient algorithms for delayed neutron generation, neutron population control, and initial condition modeling. In this paper, a new MC steady-state simulation method based on time-dependent MC neutron tracking is proposed for steady-state initial condition modeling; during this process, prompt neutron sources and delayed neutron precursors for the DMC transient simulation can easily be sampled. The DMC method, including the proposed time-dependent DMC steady-state simulation method, has been implemented in McCARD and applied for two-dimensional core kinetics problems in the time-dependent neutron transport benchmark C5G7-TD. The McCARD DMC calculation results show good agreement with results of a deterministic transport analysis code, nTRACER.

  6. CF3+ fragmentation by electron impact ionization of perfluoro-propyl-vinyl-ethers, C5F10O, in gas phase

    Science.gov (United States)

    Kondo, Yusuke; Ishikawa, Kenji; Hayashi, Toshio; Miyawaki, Yudai; Takeda, Keigo; Kondo, Hiroki; Sekine, Makoto; Hori, Masaru

    2015-04-01

    The gas phase fragmentations of perfluoro-propyl-vinyl ether (PPVE, C5F10O) are studied experimentally. Dominant fragmentations of PPVE are found to be the result of a dissociative ionization reaction, i.e., CF3+ via direct bond cleavage, and C2F3O- and C3F7O- via electron attachment. Regardless of the appearance energy of around 14.5 eV for the dissociative ionization of CF3+, the observed ion efficiency for the CF3+ ion was extremely large the order of 10-20 cm-2, compared with only 10-21 cm-2 for the other channels. PPVE characteristically generated CF3+ as the largest abundant ion are advantageous for use of feedstock gases in plasma etching processes.

  7. Synthesis of novel 1H-1,2,3-triazole tethered C-5 substituted uracil-isatin conjugates and their cytotoxic evaluation

    KAUST Repository

    Kumar, Kewal; Sagar, Sunil; Esau, Luke; Kaur, Mandeep; Kumar, Vipan

    2012-01-01

    The present manuscript describes the synthesis of uracil-isatin hybrids via azide-alkyne cycloadditions and their cytotoxic evaluation against three human cancer cell lines viz. HeLa (cervix), MCF-7 (breast) and DU145 (prostate) using MTT assay. The evaluation studies revealed the dependence of cytotoxicity on C-5 substituents of both uracil and isatin as well as the alkyl chain length with compounds 6g and 6k showing IC50 values 18.21 and 13.90 μM respectively against DU145 cell lines. Most of the synthesized conjugates exhibited considerable selectivity against MCF-7 and DU145 cell lines. © 2012 Elsevier Masson SAS. All rights reserved.

  8. Synthesis of novel 1H-1,2,3-triazole tethered C-5 substituted uracil-isatin conjugates and their cytotoxic evaluation

    KAUST Repository

    Kumar, Kewal

    2012-12-01

    The present manuscript describes the synthesis of uracil-isatin hybrids via azide-alkyne cycloadditions and their cytotoxic evaluation against three human cancer cell lines viz. HeLa (cervix), MCF-7 (breast) and DU145 (prostate) using MTT assay. The evaluation studies revealed the dependence of cytotoxicity on C-5 substituents of both uracil and isatin as well as the alkyl chain length with compounds 6g and 6k showing IC50 values 18.21 and 13.90 μM respectively against DU145 cell lines. Most of the synthesized conjugates exhibited considerable selectivity against MCF-7 and DU145 cell lines. © 2012 Elsevier Masson SAS. All rights reserved.

  9. Site-specific conjugation and labelling of prostate antibody 7E11C5.3 (CYT-351) with technetium-99m

    International Nuclear Information System (INIS)

    Stalteri, M.A.; Mather, S.J.; Belinka, B.A.; Coughlin, D.J.; Chengazi, V.U.; Britton, K.E.

    1997-01-01

    Attachment of chelating agents to the sugar residues of antibodies for subsequent radiolabelling is an attractive approach since it may have less effect on the immunoreactivity than attachment through lysine residues, which are distributed throughout the antibody and may be present near the antigen binding site. We have attached a new hydrazide-linked chelator CYT-395 (Cytogen Corp., Princeton, N.J.) to the sugar residues of the anti-prostate monoclonal antibody 7E11C5.3 and optimised the conditions for labelling the conjugate with technetium-99m in order to compare the conjugate to 7E11C5.3 antibody labelled directly with technetium using a mercaptoethanol reduction technique. Labelling yields of 70%-90% were obtained at specific activities up to 2000 MBq/mg antibody. The stability of the technetium-labelled conjugate in plasma or to a challenge with 0.1 or 1.0 mM cysteine was similar to that of direct-labelled antibody. In nine patients with prostate cancer, the plasma clearance of the labelled conjugate followed a two-compartment model, with an average β-phase half-life of 31.4±3.9 h. The average urinary clearance at 24 h was 15.3±5.0% of the injected dose. In this group of patients there was no significant difference between the blood and urine clearance of the labelled conjugate, and the clearances of the direct-labelled antibody. (orig.). With 5 figs

  10. Identification and characterization of NDT 9513727 [N,N-bis(1,3-benzodioxol-5-ylmethyl)-1-butyl-2,4-diphenyl-1H-imidazole-5-methanamine], a novel, orally bioavailable C5a receptor inverse agonist.

    Science.gov (United States)

    Brodbeck, Robbin M; Cortright, Daniel N; Kieltyka, Andrzej P; Yu, Jianying; Baltazar, Carolyn O; Buck, Marianne E; Meade, Robin; Maynard, George D; Thurkauf, Andrew; Chien, Du-Shieng; Hutchison, Alan J; Krause, James E

    2008-12-01

    The complement system represents an innate immune mechanism of host defense that has three effector arms, the C3a receptor, the C5a receptor (C5aR), and the membrane attack complex. Because of its inflammatory and immune-enhancing properties, the biological activity of C5a and its classical receptor have been widely studied. Because specific antagonism of the C5aR could have therapeutic benefit without affecting the protective immune response, the C5aR continues to be a promising target for pharmaceutical research. The lack of specific, potent and orally bioavailable small-molecule antagonists has limited the clinical investigation of the C5aR. We report the discovery of NDT 9513727 [N,N-bis(1,3-benzodioxol-5-ylmethyl)-1-butyl-2,4-diphenyl-1H-imidazole-5-methanamine], a small-molecule, orally bioavailable, selective, and potent inverse agonist of the human C5aR. NDT 9513727 was discovered based on the integrated use of in vitro affinity and functional assays in conjunction with medicinal chemistry. NDT 9513727 inhibited C5a-stimulated responses, including guanosine 5'-3-O-(thio)triphosphate binding, Ca(2+) mobilization, oxidative burst, degranulation, cell surface CD11b expression and chemotaxis in various cell types with IC(50)s from 1.1 to 9.2 nM, respectively. In C5a competition radioligand binding experiments, NDT 9513727 exhibited an IC(50) of 11.6 nM. NDT 9513727 effectively inhibited C5a-induced neutropenia in gerbil and cynomolgus macaque in vivo. The findings suggest that NDT 9513727 may be a promising new entity for the treatment of human inflammatory diseases.

  11. Simulator tests for further development of the nuclear power plant's man-machine interface, the control room

    International Nuclear Information System (INIS)

    Becker, G.

    1988-01-01

    Methods for registration and analysis of the behavior of operators during simulated failures are described. Operator strategies and team work are addressed, as well as technical and psychological possibilities and limits of simulation experiments. The transferability of the test experiences is discussed and further projects are sketched out. 7 figs., 10 refs

  12. Experience for plant monitoring design in Italian BWR NPP and future trends in man-machine interface

    International Nuclear Information System (INIS)

    Maestri, F.; Sepielli, M.

    1987-01-01

    TMI accidental sequence and daily-gained operating experience on italian and abroad NPPs have affected in depth the approach to the design of information presentation to the Control Room staff. It has been cleared that most problems in plant operation arise from a poor and inadequate information system. The main lacks have been identified in the Control Room lay-out and information organization. This has pushed designers both to improve the Control Room environment and to better exploit the computer data processing and data presentation capabilities. The paper deals with the basic criteria for the design and the design review of a computerized system to be inserted in a hybrid Control Room in Italian 981 Mwe BWR-6 NPP, where the concepts outlined above were taken-up from the very beginning. The Control Room keeps conventional instrumentation arranged in a human-factor lay-out, according to post-TMI requirements, and adds a powerful computer-based information system for advanced alarm presentation and plant supervision during both normal and emergency conditions with high data reliability. Colour videounits and operating panels are functionally integrated to create powerful operator work-stations. Emphasis is mostly given on the revision work for video-unit displays and Man-System Communication carried out in cooperation with Halden Reactor Project human factor and plant operation experts. The work peculiarity has been a strong care on the integration between conventional and computerized information presentation, with particular regard to common information and code consistency. (author)

  13. Experiences in the application of human factors engineering to the modernization of the interface man-machine

    International Nuclear Information System (INIS)

    Valdivia Martin, C.; Fernandez Illobre, L.; Trueba Alonso, P.; Cerezal Diez, J. E.

    2014-01-01

    This paper shows some of the experience gained during the application of the IFH to the modernization of the IHM. The experience of the application of IFH in modernization and modifications of design shows a positive effect, improving the IHM associated, with the acceptance of the end user. (Author)

  14. Representation of nuclear reactor information by means of colour CRT display - a contribution to man-machine communication

    International Nuclear Information System (INIS)

    Stein, H.

    1984-01-01

    A data representing technique of special nuclear reactor measurement values by means of colour CRT display is discribed. The data of an incore measurement system are logged continously. A primary data processing is followed by a calculation of selected incore parameters. The parameters are offered the operator on a colour display completed by other important values of the conventional reactor instrumentation. The method is realized by the microprocessor system ursadat 5000, robotron 1600, and MPS 4944. The bit-serial data transmission requires a line interface working in a multipoint regime. The software consists of modules to allow the insertion in a superordinate automation system. (author)

  15. Conversion of Syngas-Derived C2+ Mixed Oxygenates to C3-C5 Olefins over ZnxZryOz Mixed Oxides Catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Colin D.; Lebarbier, Vanessa M.; Flake, Matthew D.; Ramasamy, Karthikeyan K.; Kovarik, Libor; Bowden, Mark E.; Onfroy, Thomas; Dagle, Robert A.

    2016-04-01

    In this study we report on a ZnxZryOz mixed oxide type catalyst capable of converting a syngas-derived C2+ mixed oxygenate feedstock to isobutene-rich olefins. Aqueous model feed comprising of ethanol, acetaldehyde, acetic acid, ethyl acetate, methanol, and propanol was used as representative liquid product derived from a Rh-based mixed oxygenate synthesis catalyst. Greater than 50% carbon yield to C3-C5 mixed olefins was demonstrated when operating at 400-450oC and 1 atm. In order to rationalize formation of the products observed feed components were individually evaluated. Major constituents of the feed mixture (ethanol, acetaldehyde, acetic acid, and ethyl acetate) were found to produce isobutene-rich olefins. C-C coupling was also demonstrated for propanol feedstock - a minor constituent of the mixed oxygenate feed - producing branched C6 olefins, revealing scalability to alcohols higher than ethanol following an analogous reaction pathway. Using ethanol and propanol feed mixtures, cross-coupling reactions produced mixtures of C4, C5, and C6 branched olefins. The presence of H2 in the feed was found to facilitate hydrogenation of the ketone intermediates, thus producing straight chain olefins as byproducts. While activity loss from coking is observed complete catalyst regeneration is achieved by employing mild oxidation. For conversion of the mixed oxygenate feed a Zr/Zn ratio of 2.5 and a reaction temperature of 450oC provides the best balance of stability, activity, and selectivity. X-ray diffraction and scanning transmission electron microscopy analysis reveals the presence of primarily cubic phase ZrO2 and a minor amount of the monoclinic phase, with ZnO being highly dispersed in the lattice. The presence of ZnO appears to stabilize the cubic phase resulting in less monoclinic phase as the ZnO concentration increases. Infrared spectroscopy shows the mixed oxide acid sites are characterized as primarily Lewis type acidity. The direct relationship between

  16. Local expression of global forcing factors in Lower Cretaceous, Aptian carbon isotope segment C5: El Pujal Section, Organya Basin, Catalunya, Spain.

    Science.gov (United States)

    Socorro, J.; Maurrasse, F. J.

    2017-12-01

    During the Aptian, the semi-restricted Organya Basin accumulated sediments under quasi-continuous dysoxic conditions [1]. High resolution stable carbon isotope (δ13Corg) values for 71.27 m of interbedded limestones, argillaceous limestones and marlstones of the El Pujal sequence show relatively small variability (1.65‰) fluctuating between -25.09‰ and -23.44‰ with an average of -24.02‰. This pattern is consistent with values reported for other Tethyan sections for carbon isotope segment C5 [2]. The geochemical and petrographic results of the sequence, reveal periodic enrichment of redox sensitive trace elements (V, Cr, Co, Ni, Cu, Mo, U), biolimiting (P, Fe) and major elements (Al, Si, Ti) at certain levels concurrent with episodes of enhanced organic carbon preservation (TOC). Inorganic carbonate (TIC) dilution due to significant clay fluxes is also evident along these intervals as illustrated by the strong negative correlation with Al (r = -0.91). Microfacies characterized by higher pyrite concentration, impoverished benthic fauna and lower degree of bioturbation index (3) are in accord with geochemical proxies. When combined, these results suggest recurrent intermittent dysoxic conditions associated with episodic increases of terrigenous supplies by riverine fluxes, which are in agreement with results reported for the basal segment of the section (0-13.77m) [3]. Concurrently, δ13Corg values show a positive correlation with TIC (r = 0.50) and a negative correlation with TOC (r = -0.46), thus showing more negative values corresponding with intervals of highest terrestrial influences, which were previously correlated with higher inputs of higher chain (>nC25) n-alkanes [3]. Hence, the results highlight the local expression of the δ13Corg signal related to higher inputs of terrestrial vegetation linked with lower δ13Corg values modulating the global signature of segment C5. References: [1] Sanchez-Hernandez & Maurrasse, 2016. Palaeo3 441; [2] Menegatti

  17. Haldane-gap excitations in the low-Hc one-dimensional quantum antiferromagnet Ni(C5D14N2)2N3(PF6)

    International Nuclear Information System (INIS)

    Zheludev, A.; Chen, Y.; Broholm, C. L.; Honda, Z.; Katsumata, K.

    2001-01-01

    Inelastic neutron scattering on deuterated single-crystal samples is used to study Haldane-gap excitations in the new S=1 one-dimensional quantum antiferromagnet Ni(C 5 D 14 N 2 ) 2 N 3 (PF 6 ), that was recently recognized as an ideal model system for high-field studies. The Haldane gap energies Δ x =0.42(3) meV, Δ y =0.52(6) meV, and Δ z =1.9(1) meV, for excitations polarized along the a, b, and c crystallographic axes, respectively, are measured. The dispersion relation is studied for momentum transfers both along and perpendicular to the chains' direction. The in-chain exchange constant J=2.8 meV is found to be much larger than interchain coupling, J y =1.8(4)x10 -3 meV and J x =4(3)x10 -4 meV, along the b and a axes, respectively. The results are discussed in the context of future experiments in high magnetic fields

  18. Distal nerve transfer versus supraclavicular nerve grafting: comparison of elbow flexion outcome in neonatal brachial plexus palsy with C5-C7 involvement.

    Science.gov (United States)

    Heise, Carlos O; Siqueira, Mario G; Martins, Roberto S; Foroni, Luciano H; Sterman-Neto, Hugo

    2017-09-01

    Ulnar and median nerve transfers to arm muscles have been used to recover elbow flexion in infants with neonatal brachial plexus palsy, but there is no direct outcome comparison with the classical supraclavicular nerve grafting approach. We retrospectively analyzed patients with C5-C7 neonatal brachial plexus palsy submitted to nerve surgery and recorded elbow flexion recovery using the active movement scale (0-7) at 12 and 24 months after surgery. We compared 13 patients submitted to supraclavicular nerve grafting with 21 patients submitted to distal ulnar or median nerve transfer to biceps motor branch. We considered elbow flexion scores of 6 or 7 as good results. The mean elbow flexion score and the proportion of good results were better using distal nerve transfers than supraclavicular grafting at 12 months (p nerve grafting at 12 months showed good elbow flexion recovery after ulnar nerve transfers. Distal nerve transfers provided faster elbow flexion recovery than supraclavicular nerve grafting, but there was no significant difference in the outcome after 24 months of surgery. Patients with failed supraclavicular grafting operated early can still benefit from late distal nerve transfers. Supraclavicular nerve grafting should remain as the first line surgical treatment for children with neonatal brachial plexus palsy.

  19. Pharmacokinetics of the FO23C5 anti-CEA antibody fragment labelled with 99Tcm and 111In: a comparison in patients

    International Nuclear Information System (INIS)

    Hnatowich, D.J.; Mardirossian, G.; Rusckowski, M.; Roy, S.; Busche, H.; Griffin, T.W.; Brill, A.B.

    1993-01-01

    The FO23C5 anti-carcinoembryonic antigen (CEA) F(ab') 2 antibody was radiolabelled with sup(111)In via diethylenetriaminepentaacetic acid (DTPA) and directly with 99 Tc m by stannous ion and mercaptoethanol antibody reduction to compare the pharmacokinetics of these three agents. Four patients received 15 mCi 99 Tc m -Fab' 1 week before receiving 1 mCi 111 In-F(ab') 2 . Five additional patients received only the 99 Tc m -Fab'. The biodistribution of 99 Tc m was as expected for a labelled Fab' fragment: relative to 111 In, 99 Tc m cleared rapidly from circulation and into kidneys and urine. Liver levels of 111 In and 99 Tc m were surprisingly similar at 1 day although initial 111 In levels were lower and increased while 99 Tc m levels were higher and decreased. Spleen levels were also similar. In 4/9 patients receiving 99 Tc m , hepatobiliary clearance was observed at levels which could confuse interpretation whereas this mode of clearance was observed in only 1/4 patients receiving 111 In. Image quality was superior with 111 In versus 99 Tc m at 1 day postadministration as judged by counting rates and background activity whereas the opposite was true at 2-3 h postadministration. (author)

  20. The CPT1C 5'UTR contains a repressing upstream open reading frame that is regulated by cellular energy availability and AMPK.

    Directory of Open Access Journals (Sweden)

    Ines Lohse

    Full Text Available BACKGROUND: Translational control is utilized as a means of regulating gene expression in many species. In most cases, posttranscriptional regulatory mechanisms play an important role in stress response pathways and can lead to dysfunctional physiology if blocked by mutations. Carnitine Palmitoyltransferase 1 C (CPT1C, the brain-specific member of the CPT 1 family, has previously been shown to be involved in regulating metabolism in situations of energy surplus. PRINCIPAL FINDINGS: Sequence analysis of the CPT1C mRNA revealed that it contains an upstream open reading frame (uORF in the 5' UTR of its mRNA. Using CPT1C 5' UTR/luciferase constructs, we investigated the role of the uORF in translational regulation. The results presented here show that translation from the CPT1C main open reading frame (mORF is repressed by the presence of the uORF, that this repression is relieved in response to specific stress stimuli, namely glucose deprivation and palmitate-BSA treatment, and that AMPK inhibition can relieve this uORF-dependent repression. SIGNIFICANCE: The fact that the mORF regulation is relieved in response to a specific set of stress stimuli rather than general stress response, hints at an involvement of CPT1C in cellular energy-sensing pathways and provides further evidence for a role of CPT1C in hypothalamic regulation of energy homeostasis.

  1. Physicochemical properties of manganese dioxide synthesized using C2–C5 alcohols as reducing agents and their catalytic activities for CO oxidation

    KAUST Repository

    Lee, Young-Ho

    2015-09-26

    MnO2 catalysts were synthesized in an aqueous solution of KMnO4 and C2–C5 alcohols using a simple redox method at room temperature. The crystalline structure of all samples was δ-MnO2 after being calcined at 300 °C. However, other physicochemical properties of the samples varied depending on the symmetry of the alcohols used. For the catalytic oxidation of CO, MnO2 catalysts prepared with 1° alcohols performed better than the samples prepared in 2° alcohols. Catalytic activities were correlated to the quantity of labile oxygen species of the catalysts. In CO-TPD analysis, the relative area of desorbed radical dotCO2, which is the product of the reaction between adsorbed CO and lattice oxygen species, becomes larger for MnO2 prepared with 1° alcohols than with 2° alcohols. These results were primarily resulted from the innate hydrogen dissociation behavior of alcohol in solution. The pKa was found to be an important factor in determining the physicochemical properties and catalytic activity toward CO oxidation of MnO2.

  2. Proteolysis of complement factors iC3b and C5 by the serine protease prostate-specific antigen in prostatic fluid and seminal plasma.

    Science.gov (United States)

    Manning, Michael L; Williams, Simon A; Jelinek, Christine A; Kostova, Maya B; Denmeade, Samuel R

    2013-03-15

    Prostate-specific Ag (PSA) is a serine protease that is expressed exclusively by normal and malignant prostate epithelial cells. The continued high-level expression of PSA by the majority of men with both high- and low-grade prostate cancer throughout the course of disease progression, even in the androgen-ablated state, suggests that PSA has a role in the pathogenesis of disease. Current experimental and clinical evidence suggests that chronic inflammation, regardless of the cause, may predispose men to prostate cancer. The responsibility of the immune system in immune surveillance and eventually tumor progression is well appreciated but not completely understood. In this study, we used a mass spectrometry-based evaluation of prostatic fluid obtained from diseased prostates after removal by radical prostatectomy to identify potential immunoregulatory proteins. This analysis revealed the presence of Igs and the complement system proteins C3, factor B, and clusterin. Verification of these findings by Western blot confirmed the high-level expression of C3 in the prostatic fluid and the presence of a previously uncharacterized C-terminal C3 cleavage product. Biochemical analysis of this C3 cleavage fragment revealed a putative PSA cleavage site after tyrosine-1348. Purified PSA was able to cleave iC3b and the related complement protein C5. These results suggest a previously uncharacterized function of PSA as an immunoregulatory protease that could help to create an environment hospitable to malignancy through proteolysis of the complement system.

  3. Physicochemical properties of manganese dioxide synthesized using C2–C5 alcohols as reducing agents and their catalytic activities for CO oxidation

    KAUST Repository

    Lee, Young-Ho; Park, Jung-Hyun; Shin, Chae-Ho

    2015-01-01

    MnO2 catalysts were synthesized in an aqueous solution of KMnO4 and C2–C5 alcohols using a simple redox method at room temperature. The crystalline structure of all samples was δ-MnO2 after being calcined at 300 °C. However, other physicochemical properties of the samples varied depending on the symmetry of the alcohols used. For the catalytic oxidation of CO, MnO2 catalysts prepared with 1° alcohols performed better than the samples prepared in 2° alcohols. Catalytic activities were correlated to the quantity of labile oxygen species of the catalysts. In CO-TPD analysis, the relative area of desorbed radical dotCO2, which is the product of the reaction between adsorbed CO and lattice oxygen species, becomes larger for MnO2 prepared with 1° alcohols than with 2° alcohols. These results were primarily resulted from the innate hydrogen dissociation behavior of alcohol in solution. The pKa was found to be an important factor in determining the physicochemical properties and catalytic activity toward CO oxidation of MnO2.

  4. Ionic conduction in 70-MeV C5+-ion-irradiated poly(vinylidenefluoride- co-hexafluoropropylene)-based gel polymer electrolytes

    International Nuclear Information System (INIS)

    Saikia, D.; Kumar, A.; Singh, F.; Avasthi, D.K.; Mishra, N.C.

    2005-01-01

    In an attempt to increase the Li + -ion diffusivity, poly(vinylidenefluoride-co-hexafluoropropylene)-(propylene carbonate+diethyl carbonate)-lithium perchlorate gel polymer electrolyte system has been irradiated with 70-MeV C 5+ -ion beam of nine different fluences. Swift heavy-ion irradiation shows enhancement in ionic conductivity at lower fluences and decrease in ionic conductivity at higher fluences with respect to unirradiated gel polymer electrolyte films. Maximum room-temperature (303 K) ionic conductivity is found to be 2x10 -2 S/cm after irradiation with a fluence of 10 11 ions/cm 2 . This interesting result could be attributed to the fact that for a particular ion beam with a given energy, a higher fluence provides critical activation energy for cross linking and crystallization to occur, which results in the decrease in ionic conductivity. X-ray-diffraction results show decrease in the degree of crystallinity upon ion irradiation at low fluences (≤10 11 ions/cm 2 ) and increase in crystallinity at higher fluences (>10 11 ions/cm 2 ). Analysis of Fourier-transform infrared spectroscopy results suggests the bond breaking at a fluence of 5x10 9 ions/cm 2 and cross linking at a fluence of 10 12 ions/cm 2 and corroborate conductivity and x-ray-diffraction results. Scanning electron micrographs exhibit increased porosity of the polymer electrolyte after ion irradiation

  5. SYNTHESIS AND CHARACTERIZATION OF (MU-5-C5ME5)2TI(R)CL (R = ME, ET, NORMAL-PR, CH=CH2, PH, O-NORMAL-PR) AND THEIR SALT METATHESIS REACTIONS - THERMAL-DECOMPOSITION PATHWAYS OF (MU-5-C5ME5)2TI(ME)R' (R' = ET, CH=CH2, PH, CH2PH)

    NARCIS (Netherlands)

    LUINSTRA, GA; TEUBEN, JH

    Complexes Cp*2Ti(R)Cl (Cp* = eta-5-C5Me5; R = Me (1), Et (2), n-Pr (3), CH=CH2 (4), Ph (5), O-n-Pr (6)) have been prepared by oxidation Of CP*2TiR with lead dichloride. Not every compound Cp*2Ti(R)Cl was accessible and for R = CH2CMe3 and CH2Ph reduction to Cp*2TiCl and R. was observed. Homolysis of

  6. Synthesis and Characterization of (η5-C5Me5)2Ti(R)Cl (R = Me, Et, n-Pr, CH=CH2, Ph, O-n-Pr) and Their Salt Metathesis Reactions. Thermal Decomposition Pathways of (η5-C5Me5)2Ti(Me)R' (R' = Et, CH=CH2, Ph, CH2Ph)

    NARCIS (Netherlands)

    Luinstra, Gerrit A.; Teuben, Jan H.

    1992-01-01

    Complexes Cp*2Ti(R)Cl (Cp* = η5-C5Me5; R = Me (1), Et (2), n-Pr (3), CH=CH2 (41, Ph (5), O-n-Pr (6)) have been prepared by oxidation of Cp*2TiR with lead dichloride. Not every compound Cp*2Ti(R)Cl was accessible and for R = CH2CMe3 and CH2Ph reduction to Cp*2TiCl and R· was observed. Homolysis of

  7. OXIDATIVE ALKYLATION OF (ETA-5-C5ME5)2TIR (R=CL, ME, ET, CH=CH2, PH, OME, N=C(H)TERT-BU) TO (ETA-5-C5ME5)2TI(ME)R BY GROUP-12 ORGANOMETALLIC COMPOUNDS MME2

    NARCIS (Netherlands)

    LUINSTRA, GA; TEUBEN, JH

    1991-01-01

    Oxidative alkylation of Cp*2TiX (Cp*: eta-5-C5Me5; X = OMe, Cl, N = C(H)tBu) and Cp*2TiMe by CdMe2 or ZnMe2 gives diamagnetic Cp*2Ti(Me)X and Cp*2TiMe2 respectively, and cadmium or zinc. The reactions of Cp*2TiR (R = Et, CH = CH2, Ph) with MMe2 (M = Cd, Zn) give statistical mixtures of Cp*2Ti(Me)R,

  8. Encapsulation of a trinuclear silver(I) cluster by two imido-nitrido metalloligands [{Ti(eta5-C5Me5)(micro-NH)}3(micro3-N)].

    Science.gov (United States)

    Martín, Avelino; Martínez-Espada, Noelia; Mena, Miguel; Yélamos, Carlos

    2007-07-28

    Treatment of the metalloligand [{Ti(eta(5)-C(5)Me(5))(micro-NH)}(3)(micro(3)-N)] with silver(i) trifluoromethanesulfonate in different molar ratios gives the ionic compounds [Ag{(micro(3)-NH)(3)Ti(3)(eta(5)-C(5)Me(5))(3)(micro(3)-N)}(2)][O(3)SCF(3)] and [Ag{(micro(3)-NH)(3)Ti(3)(eta(5)-C(5)Me(5))(3)(micro(3)-N)}][O(3)SCF(3)] or the triangular silver cluster [(CF(3)SO(2)O)(3)Ag(3){(micro(3)-NH)(3)Ti(3)(eta(5)-C(5)Me(5))(3)(micro(3)-N)}(2)] in which each face is capped by a metalloligand.

  9. SYNTHESIS OF NEW BIS(CYCLOPENTADIENYL)YTTRIUM COMPLEXES WITH ETHER FUNCTIONALIZED CYCLOPENTADIENYL LIGANDS - CRYSTAL-STRUCTURE OF [(C(5)H(4)CH(2)CH(2)OME)(2)Y(MU-H)(2)BH2

    NARCIS (Netherlands)

    LASKE, DA; DUCHATEAU, R; TEUBEN, JH; SPEK, AL

    1993-01-01

    Treatment of C(5)H(4)CH(2)CH(2)OMe.Li(TMEDA) (TMEDA = N, N, N', N' tetramethylethylenediamine) with half an equivalent of YCl3(THF)(3.5) in toluene afforded the salt- and solvent-free dimeric biscyclopentadienyl yttrium chloride [(C(5)H(4)CH(2)CH(2)OMe)(2)Y-(mu-Cl)](2) (1). Reaction of 1 with one

  10. Complement factor 5 (C5) p.A252T mutation is prevalent in, but not restricted to, sub-Saharan Africa: implications for the susceptibility to meningococcal disease.

    Science.gov (United States)

    Franco-Jarava, C; Comas, D; Orren, A; Hernández-González, M; Colobran, R

    2017-08-01

    Complement C5 deficiency (C5D) is a rare primary immunodeficiency associated with recurrent infections, particularly meningitis, by Neisseria species. To date, studies to elucidate the molecular basis of hereditary C5D have included fewer than 40 families, and most C5 mutations (13 of 17) have been found in single families. However, the recently described C5 p.A252T mutation is reported to be associated with approximately 7% of meningococcal disease cases in South Africa. This finding raises the question of whether the mutation may be prevalent in other parts of Africa or other continental regions. The aim of this study was to investigate the prevalence of C5 p.A252T in Africa and other regions and discuss the implications for prophylaxis against meningococcal disease. In total, 2710 samples from healthy donors within various populations worldwide were analysed by quantitative polymerase chain reaction (qPCR) assay to detect the C5 p.A252T mutation. Eleven samples were found to be heterozygous for p.A252T, and nine of these samples were from sub-Saharan African populations (allele frequency 0·94%). Interestingly, two other heterozygous samples were from individuals in populations outside Africa (Israel and Pakistan). These findings, together with data from genomic variation databases, indicate a 0·5-2% prevalence of the C5 p.A252T mutation in heterozygosity in sub-Saharan Africa. Therefore, this mutation may have a relevant role in meningococcal disease susceptibility in this geographical area. © 2017 British Society for Immunology.

  11. Concentrations of higher dicarboxylic acids C5–C13 in fresh snow samples collected at the High Alpine Research Station Jungfraujoch during CLACE 5 and 6

    Directory of Open Access Journals (Sweden)

    K. Sieg

    2009-03-01

    Full Text Available Samples of freshly fallen snow were collected at the high alpine research station Jungfraujoch (Switzerland in February and March 2006 and 2007, during the Cloud and Aerosol Characterization Experiments (CLACE 5 and 6. In this study a new technique has been developed and demonstrated for the measurement of organic acids in fresh snow. The melted snow samples were subjected to solid phase extraction and resulting solutions analysed for organic acids by HPLC-MS-TOF using negative electrospray ionization. A series of linear dicarboxylic acids from C5 to C13 and phthalic acid, were identified and quantified. In several samples the biogenic acid pinonic acid was also observed. In fresh snow the median concentration of the most abundant acid, adipic acid, was 0.69 μg L−1 in 2006 and 0.70 μg L−1 in 2007. Glutaric acid was the second most abundant dicarboxylic acid found with median values of 0.46 μg L−1 in 2006 and 0.61 μg L−1 in 2007, while the aromatic acid phthalic acid showed a median concentration of 0.34 μg L−1 in 2006 and 0.45 μg L−1 in 2007. The concentrations in the samples from various snowfall events varied significantly, and were found to be dependent on the back trajectory of the air mass arriving at Jungfraujoch. Air masses of marine origin showed the lowest concentrations of acids whereas the highest concentrations were measured when the air mass was strongly influenced by boundary layer air.

  12. Standard Molar Enthalpy of Formation of RE(C5H8NS2)3(C12H8N2)

    Institute of Scientific and Technical Information of China (English)

    Meng Xiangxin; Shuai Qi; Chen Sanping; Xie Gang; Gao Shengli; Shi Qizhen

    2005-01-01

    Four solid ternary complexes of RE (C5H8NS2)3(C12H8N2) (RE=Eu, Gd, Tb, Dy) were synthesized in absolute ethanol by rare earth chloride low hydrate with the mixed ligands of ammonium pyrrolidinedi-thiocarbamate (APDC) and 1, 10-phenanthroline*H2O (o-phen*H2O) in the ordinary laboratory atmosphere without any cautions against moisture or air sensitivity. IR spectra of the complexes show that the RE3+ coordinated with six sulfur atoms of three PDC- and two nitrogen atoms of o-phen*H2O. It was assumed that the coordination number of RE3+ is eight. The constant-volume combustion energies of the complexes, ΔcU, were determined as (-16937.88±9.79 ), (-17588.79±8.62 ), (-17747.14±8.25 ) and (-17840.37±8.87 ) kJ*mol-1, by a precise rotating-bomb calorimeter at 298.15 K. Its standard molar enthalpies of combustion, ΔcHθm, and standard molar enthalpies of formation, ΔfHθm, were calculated as (-16953.37±9.79), (-17604.28±8.62), (-17762.63±8.25), (-17855.86±8.87) kJ*mol-1 and (-857.04±10.52), (-282.43±9.58), (-130.08±9.13), (-55.75±9.83) kJ*mol-1.

  13. Enthalpies of ligand substitution for [Mo(η5C5H5)(CO)2(NO)] – The role of π-bonding effects in metal–ligand bond strengths

    International Nuclear Information System (INIS)

    Majumdar, Subhojit; Captain, Burjor; Cazin, Catherine S.J.; Nolan, Steven P.; Hoff, Carl D.

    2014-01-01

    Graphical abstract: - Highlights: • Enthalpies of ligand substitution are measured for Mo(C 5 H 5 )(CO) 2 (NO). • Phosphines and N-heterocyclic carbenes are stronger ligands and displace CO. • Backbonding to π ∗ orbitals is an important part of complex stability. • FTIR studies show shifts to lower wavenumbers of ν-CO and ν-NO. • Structural studies show lengthening of the C-O and N-O bonds. - Abstract: Enthalpies of ligand substitution for [Mo(η 5 -C 5 H 5 )(CO) 2 (NO)] producing [Mo(η 5 -C 5 H 5 )Mo(CO)(L)(NO)] have been measured by solution calorimetry at 30 °C in THF for L = P(OMe) 3 2 2 Ph 3 (SIPr = 1,3-bis(2,6-bis(diisopropylphenyl)imidazolinylidene; IPr = 1,3-bis(2,6-bis(diisopropylphenyl)-imidazol-2-ylidene)). The accepting metal fragment [Mo(η 5 -C 5 H 5 )(CO)(NO)] has a vacant site containing strongly π-accepting carbonyl and nitrosyl ligands and this is shown to influence the stability of the product complex. Infrared studies of both ν CO and ν NO show that metal-to-ligand backbonding increases in the order P(OMe) 3 3 5 -C 5 H 5 )(CO)(IPr)(NO)] and [Mo(η 5 -C 5 H 5 )(CO)(SIPr)(NO)] are reported

  14. Molecular structures and excited states of CpM(CO)(2) (Cp = eta(5)-C(5)H(5); M = Rh, Ir) and [Cl(2)Rh(CO)(2)](-). Theoretical evidence for a competitive charge transfer mechanism.

    Science.gov (United States)

    Hu, Zhenming; Boyd, Russell J; Nakatsuji, Hiroshi

    2002-03-20

    Molecular structures and excited states of CpM(CO)(2) (Cp = eta(5)-C(5)H(5); M = Rh, Ir) and [Cl(2)Rh(CO)(2)](-) complexes have been investigated using the B3LYP and the symmetry-adapted cluster (SAC)/SAC-configuration interaction (SAC-CI) theoretical methods. All the dicarbonyl complexes have singlet ground electronic states with large singlet-triplet separations. Thermal dissociations of CO from the parent dicarbonyls are energetically unfavorable. CO thermal dissociation is an activation process for [Cl(2)Rh(CO)(2)](-) while it is a repulsive potential for CpM(CO)(2). The natures of the main excited states of CpM(CO)(2) and [Cl(2)Rh(CO)(2)](-) are found to be quite different. For [Cl(2)Rh(CO)(2)](-), all the strong transitions are identified to be metal to ligand CO charge transfer (MLCT) excitations. A significant feature of the excited states of CpM(CO)(2) is that both MLCT excitation and a ligand Cp to metal and CO charge transfer excitation are strongly mixed in the higher energy states with the latter having the largest oscillator strength. A competitive charge transfer excited state has therefore been identified theoretically for CpRh(CO)(2) and CpIr(CO)(2). The wavelength dependence of the quantum efficiencies for the photoreactions of CpM(CO)(2) reported by Lees et al. can be explained by the existence of two different types of excited states. The origin of the low quantum efficiencies for the C-H/S-H bond activations of CpM(CO)(2) can be attributed to the smaller proportion of the MLCT excitation in the higher energy states.

  15. Tinnunculite, C5H4N4O3 · 2H2O: Occurrences on the Kola Peninsula and Redefinition and Validation as a Mineral Species

    Science.gov (United States)

    Pekov, I. V.; Chukanov, N. V.; Yapaskurt, V. O.; Belakovskiy, D. I.; Lykova, I. S.; Zubkova, N. V.; Shcherbakova, E. P.; Britvin, S. N.; Chervonnyi, A. D.

    2017-12-01

    Based on a study of samples found in the Khibiny (Mt. Rasvumchorr: the holotype) and Lovozero (Mts Alluaiv and Vavnbed) alkaline complexes on the Kola Peninsula, Russia, tinnunculite was approved by the IMA Commission on New Minerals, Nomenclature, and Classification as a valid mineral species (IMA no. 2015-02la) and, taking into account a revisory examination of the original material from burnt dumps of coal mines in the southern Urals, it was redefined as crystalline uric acid dihydrate (UAD), C5H4N4O3 · 2H2O. Tinnunculite is poultry manure mineralized in biogeochemical systems, which could be defined as "guano microdeposits." The mineral occurs as prismatic or tabular crystals up to 0.01 × 0.1 × 0.2 mm in size and clusters of them, as well as crystalline or microglobular crusts. Tinnunculite is transparent or translucent, colorless, white, yellowish, reddish or pale lilac. Crystals show vitreous luster. The mineral is soft and brittle, with a distinct (010) cleavage. D calc = 1.68 g/cm3 (holotype). Tinnunculite is optically biaxial (-), α = 1.503(3), β = 1.712(3), γ = 1.74(1), 2 V obs = 40(10)°. The IR spectrum is given. The chemical composition of the holotype sample (electron microprobe data, content of H is calculated by UAD stoichiometry) is as follows, wt %: 37.5 O, 28.4 C, 27.0 N, 3.8 Hcalc, total 96.7. The empirical formula calculated on the basis of (C + N+ O) = 14 apfu is: C4.99H8N4.07O4.94. Tinnunculite is monoclinic, space group (by analogy with synthetic UAD) P21/ c. The unit cell parameters of the holotype sample (single crystal XRD data) are a = 7.37(4), b = 6.326(16), c = 17.59(4) Å, β = 90(1)°, V = 820(5) Å3, Z = 4. The strongest reflections in the XRD pattern ( d, Å- I[ hkl]) are 8.82-84[002], 5.97-15[011], 5.63-24[102̅, 102], 4.22-22[112], 3.24-27[114̅,114], 3.18-100[210], 3.12-44[211̅, 211], 2.576-14[024].

  16. A new and general method for the preparation of novel II-heterocyclic derivatives of ruthenium [C5Me5Ru (η6-arene)]X (arene = benzene, thiophene, 3-methylthiophene, benzothiophene, pyridine, 2.6 and 3.5-lutidine, quinoline, acridine). X-ray crystal structure of [(C5Me5)2Ru2Cl2(pyridine)2] PF6

    International Nuclear Information System (INIS)

    Chaudret, B.; Jalon, F.; Perez-Manrique, M.; Lahoz, F.; Plou, F.J.

    1990-01-01

    Zinc reduction of (Cp*RuCl 2 ) n (Cp* = C 5 Me 5 ) in acetone or THF followed by addition of 1 equivalent of an arene or aromatic heterocycle leads to compounds of general formulation [Cp* Ru(arene)]X (X = Cl, BF 4 ). Coordination of benzene is rapid and competes successfully with any other arene. Thiophene and 3-methylthiophene give stable π adducts whereas benzothiophene is coordinated through the benzene not through the heterocyclic ring. 2.6 and 3.5-lutidine coordinate through the ring, thus demonstrating an electronic rather than steric stabilization. Again, quinoline and acridine coordinate through the benzene ring. Pyridine gives an unstable π adduct in THF. A paramagnetic mixed-valence species, byproduct of the reaction in THF, has been characterized by an X-ray crystal structure determination. Crystals are triclinic, space group P-1

  17. DNA (Cytosine-C5) Methyltransferase Inhibition by Oligodeoxyribonucleotides Containing 2-(1H)-Pyrimidinone (Zebularine Aglycon) at the Enzymatic Target Site

    OpenAIRE

    van Bemmel, Dana M.; Brank, Adam S.; Eritja, Ramon; Marquez, Victor E.; Christman, Judith K.

    2009-01-01

    Aberrant cytosine methylation in promoter regions leads to gene silencing associated with cancer progression. A number of DNA methyltransferase inhibitors are known to reactivate silenced genes; including 5-azacytidine and 2-(1H)-pyrimidinone riboside (zebularine). Zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine. To determine the mechanistic basis for this difference, we carried out a detailed comparisons of the interaction between purified DNA methyltransferas...

  18. Compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2 as substitutes for SF6 to reduce global warming potential

    Directory of Open Access Journals (Sweden)

    Linlin Zhong

    2017-07-01

    Full Text Available C5F10O has recently been found to be a very promising alternative to SF6. This paper is devoted to the investigation of compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2. Firstly, the partition functions and enthalpies of formation for a few molecules (CxFy and CxFyO which are likely to exist in the mixtures, are calculated based on the G4(MP2 theory. The isomers of the above molecules are selected according to their Gibbs energy. The compositions of C5F10O-CO2-O2 mixtures are then determined using the minimization of the Gibbs free energy. Next, the thermodynamic properties (mass density, specific enthalpy, and specific heat are derived from the previously calculated compositions. Lastly, the transport coefficients (electrical conductivity, viscosity, and thermal conductivity are calculated based on Chapman-Enskog method. It is found that, as an arc quenching gas, C5F10O could not recombine into itself with the temperature decreasing down to room temperature after the arc extinction. Besides, the key species at room temperature are always CF4, CO2, and C4F6 if graphite is not considered. When taken into account, graphite will replace C4F6 as one of the dominate particles. The mixing of CO2 with C5F10O plasma significantly affects the thermodynamic properties (e.g. vanishing and/or shifting of the peaks in specific heat and transport coefficients (e.g. reducing viscosity and changing the number of peaks in thermal conductivity, while the addition of O2 with C5F10O-CO2 mixtures has no remarkable influence on both thermodynamic and transport properties.

  19. Compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2 as substitutes for SF6 to reduce global warming potential

    Science.gov (United States)

    Zhong, Linlin; Rong, Mingzhe; Wang, Xiaohua; Wu, Junhui; Han, Guiquan; Han, Guohui; Lu, Yanhui; Yang, Aijun; Wu, Yi

    2017-07-01

    C5F10O has recently been found to be a very promising alternative to SF6. This paper is devoted to the investigation of compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2. Firstly, the partition functions and enthalpies of formation for a few molecules (CxFy and CxFyO) which are likely to exist in the mixtures, are calculated based on the G4(MP2) theory. The isomers of the above molecules are selected according to their Gibbs energy. The compositions of C5F10O-CO2-O2 mixtures are then determined using the minimization of the Gibbs free energy. Next, the thermodynamic properties (mass density, specific enthalpy, and specific heat) are derived from the previously calculated compositions. Lastly, the transport coefficients (electrical conductivity, viscosity, and thermal conductivity) are calculated based on Chapman-Enskog method. It is found that, as an arc quenching gas, C5F10O could not recombine into itself with the temperature decreasing down to room temperature after the arc extinction. Besides, the key species at room temperature are always CF4, CO2, and C4F6 if graphite is not considered. When taken into account, graphite will replace C4F6 as one of the dominate particles. The mixing of CO2 with C5F10O plasma significantly affects the thermodynamic properties (e.g. vanishing and/or shifting of the peaks in specific heat) and transport coefficients (e.g. reducing viscosity and changing the number of peaks in thermal conductivity), while the addition of O2 with C5F10O-CO2 mixtures has no remarkable influence on both thermodynamic and transport properties.

  20. Compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2 as substitutes for SF6 to reduce global warming potential

    OpenAIRE

    Linlin Zhong; Mingzhe Rong; Xiaohua Wang; Junhui Wu; Guiquan Han; Guohui Han; Yanhui Lu; Aijun Yang; Yi Wu

    2017-01-01

    C5F10O has recently been found to be a very promising alternative to SF6. This paper is devoted to the investigation of compositions, thermodynamic properties, and transport coefficients of high-temperature C5F10O mixed with CO2 and O2. Firstly, the partition functions and enthalpies of formation for a few molecules (CxFy and CxFyO) which are likely to exist in the mixtures, are calculated based on the G4(MP2) theory. The isomers of the above molecules are selected according to their Gibbs en...

  1. Zoledronate complexes. III. Two zoledronate complexes with alkaline earth metals: [Mg(C(5)H(9)N(2)O(7)P(2))(2)(H(2)O)(2)] and [Ca(C(5)H(8)N(2)O(7)P(2))(H(2)O)](n).

    Science.gov (United States)

    Freire, Eleonora; Vega, Daniel R; Baggio, Ricardo

    2010-06-01

    Diaquabis[dihydrogen 1-hydroxy-2-(imidazol-3-ium-1-yl)ethylidene-1,1-diphosphonato-kappa(2)O,O']magnesium(II), [Mg(C(5)H(9)N(2)O(7)P(2))(2)(H(2)O)(2)], consists of isolated dimeric units built up around an inversion centre and tightly interconnected by hydrogen bonding. The Mg(II) cation resides at the symmetry centre, surrounded in a rather regular octahedral geometry by two chelating zwitterionic zoledronate(1-) [or dihydrogen 1-hydroxy-2-(imidazol-3-ium-1-yl)ethylidene-1,1-diphosphonate] anions and two water molecules, in a pattern already found in a few reported isologues where the anion is bound to transition metals (Co, Zn and Ni). catena-Poly[[aquacalcium(II)]-mu(3)-[hydrogen 1-hydroxy-2-(imidazol-3-ium-1-yl)ethylidene-1,1-diphosphonato]-kappa(5)O:O,O':O',O''], [Ca(C(5)H(8)N(2)O(7)P(2))(H(2)O)](n), consists instead of a Ca(II) cation in a general position, a zwitterionic zoledronate(2-) anion and a coordinated water molecule. The geometry around the Ca(II) atom, provided by six bisphosphonate O atoms and one water ligand, is that of a pentagonal bipyramid with the Ca(II) atom displaced by 0.19 A out of the equatorial plane. These Ca(II) coordination polyhedra are ;threaded' by the 2(1) axis so that successive polyhedra share edges of their pentagonal basal planes. This results in a strongly coupled rhomboidal Ca(2)-O(2) chain which runs along [010]. These chains are in turn linked by an apical O atom from a -PO(3) group in a neighbouring chain. This O-atom, shared between chains, generates strong covalently bonded planar arrays parallel to (100). Finally, these sheets are linked by hydrogen bonds into a three-dimensional structure. Owing to the extreme affinity of zoledronic acid for bone tissue, in general, and with calcium as one of the major constituents of bone, it is expected that this structure will be useful in modelling some of the biologically interesting processes in which the drug takes part.

  2. Comparison of the one-electron oxidations of CO-bridged vs unbridged bimetallic complexes: Electron-transfer chemistry of Os2Cp2(CO)4 and Os2Cp∗2(μ-CO)2(CO)2 (Cp = η5-C5H5, Cp∗ = η5-C5Me5)

    KAUST Repository

    Laws, Derek R.

    2014-09-22

    The one-electron oxidations of two dimers of half-sandwich osmium carbonyl complexes have been examined by electrochemistry, spectro-electrochemistry, and computational methods. The all-terminal carbonyl complex Os2Cp2(CO)4 (1, Cp = η5-C5H5) undergoes a reversible one-electron anodic reaction at E1/2 = 0.41 V vs ferrocene in CH2Cl2/0.05 M [NBu4][B(C6F5)4], giving a rare example of a metal-metal bonded radical cation unsupported by bridging ligands. The IR spectrum of 1+ is consistent with an approximately 1:1 mixture of anti and gauche structures for the 33 e- radical cation in which it has retained all-terminal bonding of the CO ligands. Density functional theory (DFT) calculations, including orbital-occupancy-perturbed Mayer bond-order analyses, show that the highest-occupied molecular orbitals (HOMOs) of anti-1 and gauche-1 are metal-ligand delocalized. Removal of an electron from 1 has very little effect on the Os-Os bond order, accounting for the resistance of 1+ to heterolytic cleavage. The Os-Os bond distance is calculated to decrease by 0.10 å and 0.06 å as a consequence of one-electron oxidation of anti-1 and gauche-1, respectively. The CO-bridged complex Os2Cp∗2(μ-CO)2(CO)2 (Cp∗ = η5-C5Me5), trans-2, undergoes a more facile oxidation, E1/2 = -0.11 V, giving a persistent radical cation shown by solution IR analysis to preserve its bridged-carbonyl structure. However, ESR analysis of frozen solutions of 2+ is interpreted in terms of the presence of two isomers, most likely anti-2+ and trans-2+, at low temperature. Calculations show that the HOMO of trans-2 is highly delocalized over the metal-ligand framework, with the bridging carbonyls accounting for about half of the orbital makeup. The Os-Os bond order again changes very little with removal of an electron, and the Os-Os bond length actually undergoes minor shortening. Calculations suggest that the second isomer of 2+ has the anti all-terminal CO structure. (Figure Presented) © 2014 American

  3. Chelate-size effects on the structures, chemical behavior, properties, and catalytic activity of the new palladium(II)-allyl complexes [Pd(eta(3)-1-R-1-C3H4){FcCH=N-CH2-(CH2)(n)-NMe2}][PF6] {Fc = (eta(5)-C5H5)Fe(eta(5)-C5H4), n=2 or 1, and R-1 = h or ph}

    NARCIS (Netherlands)

    Pérez, S.; López, C.; Bosque, R.; Solans, X.; Font-Bardía, M.; Roig, A.; Molins, E.; van Leeuwen, P.W.N.M.; van Strijdonck, G.P.F.; Freixa, Z.

    2008-01-01

    The synthesis, X-ray crystal structures, and the study of the solution behavior of the palladium(II) allyl complexes [Pd(eta(3)-1R(1)-C3H4){FcCH=N-CH2-(CH2)(n)-NMe2}][PF6] {with Fc = (eta(5)-C5H5)Fe(eta(5)-C5H4), R-1 = H, and n = 2 (4) or 1 (5) or R-1 = Ph and n = 2 (6) or 1 (7)} are described. The

  4. Overexpression of PP2A-C5 that encodes the catalytic subunit 5 of protein phosphatase 2A in Arabidopsis confers better root and shoot development under salt conditions

    Science.gov (United States)

    Protein phosphatase 2A (PP2A) is an enzyme consisting of three subunits: a scaffolding A subunit, a regulatory B subunit and a catalytic C subunit. PP2As were shown to play diverse roles in eukaryotes. In this study, the function of the Arabidopsis PP2A-C5 gene that encodes the catalytic subunit 5 o...

  5. Isopiestic determination of the osmotic coefficient and vapour pressure of N-R-4-(N,N-dimethylamino)pyridinium tetrafluoroborate (R = C4H9, C5H11, C6H13) in the ethanol solution at T = 298.15 K

    International Nuclear Information System (INIS)

    Sardroodi, Jaber Jahanbin; Atabay, Maryam; Azamat, Jafar

    2012-01-01

    Highlights: ► The osmotic coefficients of the solutions of ionic liquid in ethanol have been measured. ► Measured osmotic coefficients were correlated using Pitzer, e-NRTL and NRF models and polynomial equation. ► Vapour pressures were evaluated from the correlated osmotic coefficients. - Abstract: Osmotic coefficients of the solutions of room temperature ionic liquid N-R-4-(N,N-dimethylamino)pyridinium tetrafluoroborate (R = C 4 H 9 , C 5 H 11 , C 6 H 13 ) in ethanol have been measured at T = 298.15 K by the isopiestic method. The experimental osmotic coefficients have been correlated using the ion interaction model of Pitzer, electrolyte non-random two liquid (e-NRTL) model of Chen, non-random factor (NRF) and a fourth-order polynomial in terms of molality. The vapour pressures of the solutions studied have been evaluated from the osmotic coefficients.

  6. Nuclear safety after Three Mile Island and Chernobyl

    International Nuclear Information System (INIS)

    Ballard, G.M.

    1988-01-01

    This book contains the proceedings on nuclear safety after Three Mile island and Chernobyl. Topics covered include: Design for safety; Man-machine interaction; Source terms and consequence; and accident response

  7. Design of music player based on MCU STC12C5A60S2%一种基于51单片机的音乐播放器的设计

    Institute of Scientific and Technical Information of China (English)

    何谐; 唐大权; 张淑廷; 陈雪

    2014-01-01

    The hardware design method of the music player based on MCU STC12C5A60S2 is introduced in this paper. The program design of the music player based on principle of FAT32 file system is studied. In the music player, MCU STC12C5A60S2 is taken as a main controller,SD card as a memory medium of music files and VS1003 chip as a decoder unit. When the player is running,MCU STC12C5A60S2 reads the music file from the SD card and continuously transfers data flow to VS1003 for decoding. In the meantime,the OLED liquid crystal display shows the message of the music in real time. The tested results from experiments show the music player can play the music files in multiple formats fluently if the player is connected with ear phone.%主要介绍一种基于51单片机的音乐播放器的硬件设计方法,并研究在FAT32文件系统下音乐播放器的程序设计。该音乐播放器采用STC12C5A60S2单片机为主控制器,SD卡作为音乐文件的存储介质,VS1003芯片作为解码器。STC12C5A60S2单片机从 SD卡中读取音乐文件,并不断将数据流传送至VS1003解码,最后连接耳机播放,同时STC12C5A60S2连接OLED液晶显示屏实时显示音乐播放信息。实验表明,该音乐播放器连接耳机能流畅播放多种格式的音乐文件。

  8. Instrumental interaction

    OpenAIRE

    Luciani , Annie

    2007-01-01

    International audience; The expression instrumental interaction as been introduced by Claude Cadoz to identify a human-object interaction during which a human manipulates a physical object - an instrument - in order to perform a manual task. Classical examples of instrumental interaction are all the professional manual tasks: playing violin, cutting fabrics by hand, moulding a paste, etc.... Instrumental interaction differs from other types of interaction (called symbolic or iconic interactio...

  9. Calculations of total electron-impact ionization cross sections for Fluoroketone C5F10O and Fluoronitrile C4F7N using modified Deutsch-Märk formula

    Science.gov (United States)

    Xiong, Jiayu; Li, Xingwen; Wu, Jian; Guo, Xiaoxue; Zhao, Hu

    2017-11-01

    Both fluoroketone C5F10O and fluoronitrile C4F7N are promising substitute gases for SF6. The electron-impact ionization cross sections for these two gases are calculated using the Deutsch-Märk (DM) formula and its modified method. The necessary molecular geometry optimization and electron population were determined by ab initio calculation, which was performed with quantum chemistry code. The level of calculation, including the theoretical method and basis-set, are carefully determined. To eliminate the drawbacks of the DM formula, a modified DM formula is set in this paper. The modified DM formula, of which the weighting factors are changed, has a better agreement with the experimental data on both the peak and shape of the cross-section curves. The results calculated by DM formula and modified DM formula are given as references to fill in gaps in further research into C5F10O and C4F7N.

  10. Technology Insertion-Engineering Services Process Characterization. Task Order No. 1. Book 2 of 5. Database Documentation Book. OO-ALC MANPRA (C5 Main Landing Gear - WCD’S)

    Science.gov (United States)

    1989-12-15

    ORDER NO. 1 BOOK 2 OF 5 DATABASE DOCUMENTATION BOOK 00-ALC MANPRA *(C5 MAIN LANDING GEAR- WCD’S) CONTRACT SUMMARY REPORT , 15 DECEMBER 1989 ’.1 - CONTRACT...ORIQ 1 . T A 1~4 M E* X C I" El 1 -:11 E:XCE:EI:IIE:U RE:CORI’ RFiS CvAUiS FOREXE IN REWORK LI[MITS Ill FINAL DESTINATION 22. COOROINATION/INITIATING MCC

  11. Tailoring of the structure of Pt/WO3–ZrO2 catalyst for high activity in skeletal isomerization of C5–C6 paraffins under industrially relevant conditions

    Czech Academy of Sciences Publication Activity Database

    Hidalgo, J. M.; Kaucký, Dalibor; Bortnovsky, O.; Sobalík, Zdeněk; Černý, R.

    2015-01-01

    Roč. 41, č. 12 (2015), s. 9425-9437 ISSN 0922-6168 R&D Projects: GA MPO FR-TI3/316 Institutional support: RVO:61388955 Keywords : Pt/WOx–ZrO2 catalyst * Isomerization of C5–C6 paraffins * Heteroatom-substituted zirconia Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.833, year: 2015

  12. Ab initio/GIAO-CCSD(T) study of structures, energies, and 13C NMR chemical shifts of C4H7(+) and C5H9(+) ions: relative stability and dynamic aspects of the cyclopropylcarbinyl vs bicyclobutonium ions.

    Science.gov (United States)

    Olah, George A; Surya Prakash, G K; Rasul, Golam

    2008-07-16

    The structures and energies of the carbocations C 4H 7 (+) and C 5H 9 (+) were calculated using the ab initio method. The (13)C NMR chemical shifts of the carbocations were calculated using the GIAO-CCSD(T) method. The pisigma-delocalized bisected cyclopropylcarbinyl cation, 1 and nonclassical bicyclobutonium ion, 2 were found to be the minima for C 4H 7 (+) at the MP2/cc-pVTZ level. At the MP4(SDTQ)/cc-pVTZ//MP2/cc-pVTZ + ZPE level the structure 2 is 0.4 kcal/mol more stable than the structure 1. The (13)C NMR chemical shifts of 1 and 2 were calculated by the GIAO-CCSD(T) method. Based on relative energies and (13)C NMR chemical shift calculations, an equilibrium involving the 1 and 2 in superacid solutions is most likely responsible for the experimentally observed (13)C NMR chemical shifts, with the latter as the predominant equilibrating species. The alpha-methylcyclopropylcarbinyl cation, 4, and nonclassical bicyclobutonium ion, 5, were found to be the minima for C 5H 9 (+) at the MP2/cc-pVTZ level. At the MP4(SDTQ)/cc-pVTZ//MP2/cc-pVTZ + ZPE level ion 5 is 5.9 kcal/mol more stable than the structure 4. The calculated (13)C NMR chemical shifts of 5 agree rather well with the experimental values of C 5H 9 (+).

  13. Weak interactions

    International Nuclear Information System (INIS)

    Chanda, R.

    1981-01-01

    The theoretical and experimental evidences to form a basis for Lagrangian Quantum field theory for Weak Interactions are discussed. In this context, gauge invariance aspects of such interactions are showed. (L.C.) [pt

  14. Intermolecular interactions

    International Nuclear Information System (INIS)

    Kaplan, I.G.; Rodimova, O.B.; AN SSSR, Tomsk. Inst. Optiki Atmosfery)

    1978-01-01

    The present state of the intermolecular interaction theory is described. The general physical picture of the molecular interactions is given, the relative contributions of interactions of different types are analyzed (electrostatic, resonance, induction, dispersion, relativistic, magnetostatic and exchange), and the main ones in each range of separations are picked out. The methods of the potential curve calculations are considered, specific for definite separations between the interacting systems. The special attention is paid to the analysis of approximations used in different theoretical calculation methods

  15. Synthesis of phenobarbital 14C - 5 (1964)

    International Nuclear Information System (INIS)

    Benakis, A.

    1964-01-01

    Phenobarbital (Luminal), well known as a hypnotic agent, plays also an important role in the medical treatment of epilepsy. With the object of studying the metabolism of this medicament in the living system, the synthesis of phenobarbital marked in position 2 of the pyrimidinic ring has been effected. It has been necessary, in order to carry out to research into the metabolism of this product, to have a phenobarbital marked in position 5 of the pyrimidinic ring. This synthesis, making it possible to introduce C-14 into position 5 of the pyrimidinic ring, consists of seven stages starting from C-14 carboxyl benzoic acid having a specific activity of 25 mc/mM. The melting point of the final product is 172 - 173 deg. C and its specific activity is 11.15 mc/mM. The overall radioactive yield of the pure product, with respect to the original benzoic acid (10 mM) is of the order of 3 per cent. The purity of the product has been controlled by paper chromatography; it is of the order of 99 per cent. (author) [fr

  16. C-5 Confined Space Technical Guidance Document

    National Research Council Canada - National Science Library

    Kapranos, Sophia

    2002-01-01

    ...) strict Technical Order (TO) and Operating Instruction (0I) directives. The TOs and OIs govern procedures such as lockout/tagout and system checks prior to entering the various areas of an aircraft.

  17. Effective interactions

    International Nuclear Information System (INIS)

    Elliott, J.P.

    1981-01-01

    This chapter attempts to describe and compare some of the more important nucleon-nucleon interactions that have been used in nuclear structure calculations, and to relate them where possible to the real nucleon-nucleon interaction. Explains that different interactions have been used depending on whether one is fitting to total binding energies and densities with a Hartree Fock (HF) calculation or fitting to spectra and spectroscopic data in a shell model calculation. Examines both types of calculation after two preliminary sections concerned with notation and with the philosophy underlying the use of model spaces and effective interactions. Discusses Skyrme interactions, finite range interactions, small model space, large model space, and the Sussex potential matrix elements. Focuses on the more empirical approaches in which a simple form is chosen for the effective interaction in a given model space and the parameters are deduced from fitting many-body data

  18. Study on in vivo examination of tumors of epithelial origin for the monoclonales antibodies ior c5, ior egf/r3 and humanized hr3, by using inmunogammagraphy technique

    International Nuclear Information System (INIS)

    Ramos Suzarte, Mayra; Iznaga Escobar, Normando; Torres Gemil, Olga; Rodriguez Obaya, Teresita; Perez Rodriguez, Rolando; Rodriguez Mesa, Nelson; Oliva Gonzalez, Juan P.; Perera Pintado, Alejandro; Torres Aroche, Leonel; Batista Cuellar, Juan; Gonzalez Lazo, Nery; Cruz, Teresa; Leonard, Ibrahim; Neninger, Elia

    2003-01-01

    The present work is aimed at demonstrating that humanized hR3 and ior egf/r3, ior c5, ior cea-1 murine monoclonal antibodies (mAbs) can identify primary neoplasia lesions, Their post-surgical received and metastasis by using immunography, Results showed that above mentioned mAbs are useful for the diagnosis of epithelial tumors, their metastasis and post-surgical received. this technique becomes a concept prove for further use of mAbs in cancer therapy

  19. Weak interactions

    International Nuclear Information System (INIS)

    Ogava, S.; Savada, S.; Nakagava, M.

    1983-01-01

    The problem of the use of weak interaction laws to study models of elementary particles is discussed. The most typical examples of weak interaction is beta-decay of nucleons and muons. Beta-interaction is presented by quark currents in the form of universal interaction of the V-A type. Universality of weak interactions is well confirmed using as examples e- and μ-channels of pion decay. Hypothesis on partial preservation of axial current is applicable to the analysis of processes with pion participation. In the framework of the model with four flavours lepton decays of hadrons are considered. Weak interaction without lepton participation are also considered. Properties of neutral currents are described briefly

  20. Floor interaction

    DEFF Research Database (Denmark)

    Petersen, Marianne Graves; Krogh, Peter; Ludvigsen, Martin

    2005-01-01

    Within architecture, there is a long tradition of careful design of floors. The design has been concerned with both decorating floors and designing floors to carry information. Ubiquitous computing technology offers new opportunities for designing interactive floors. This paper presents three...... different interactive floor concepts. Through an urban perspective it draws upon the experiences of floors in architecture, and provides a set of design issues for designing interactive floors....