Sample records for c-fos du systeme

  1. Growth of peripheral and central nervous system tumors is supported by cytoplasmic c-Fos in humans and mice.

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    David C Silvestre

    Full Text Available BACKGROUND: We have previously shown that the transcription factor c-Fos is also capable of associating to endoplasmic reticulum membranes (ER and activating phospholipid synthesis. Herein we examined phospholipid synthesis status in brain tumors from human patients and from NPcis mice, an animal model of the human disease Neurofibromatosis Type 1 (NF1. PRINCIPAL FINDINGS: In human samples, c-Fos expression was at the limit of detection in non-pathological specimens, but was abundantly expressed associated to ER membranes in tumor cells. This was also observed in CNS of adult tumor-bearing NPcis mice but not in NPcis fos(-/- KO mice. A glioblastoma multiforme and a malignant PNS tumor from a NF1 patient (MPNST showed a 2- and 4- fold c-Fos-dependent phospholipid synthesis activation, respectively. MPNST samples also showed increased cell proliferation rates and abundant c-Fos expression. CONCLUSIONS: Results highlight a role of cytoplasmic c-Fos as an activator of phospholipid synthesis in events demanding high rates of membrane biogenesis as occurs for the exacerbated growth of tumors cells. They also disclose this protein as a potential target for controlling tumor growth in the nervous system.

  2. Environmental enrichment and gut inflammation modify stress-induced c-Fos expression in the mouse corticolimbic system.

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    Florian Reichmann

    Full Text Available Environmental enrichment (EE has a beneficial effect on rodent behaviour, neuronal plasticity and brain function. Although it may also improve stress coping, it is not known whether EE influences the brain response to an external (psychological stressor such as water avoidance stress (WAS or an internal (systemic stressor such as gastrointestinal inflammation. This study hence explored whether EE modifies WAS-induced activation of the mouse corticolimbic system and whether this stress response is altered by gastritis or colitis. Male C67BL/6N mice were housed under standard or enriched environment for 9 weeks, after which they were subjected to a 1-week treatment with oral iodoacetamide to induce gastritis or oral dextran sulfate sodium to induce colitis. Following exposure to WAS the expression of c-Fos, a marker of neuronal activation, was measured by immunocytochemistry. EE aggravated experimentally induced colitis, but not gastritis, as shown by an increase in the disease activity score and the colonic myeloperoxidase content. In the brain, EE enhanced the WAS-induced activation of the dentate gyrus and unmasked an inhibitory effect of gastritis and colitis on WAS-evoked c-Fos expression within this part of the hippocampus. Conversely, EE inhibited the WAS-evoked activation of the central amygdala and prevented the inhibitory effect of gastritis and colitis on WAS-evoked c-Fos expression in this region. EE, in addition, blunted the WAS-induced activation of the infralimbic cortex and attenuated the inhibitory effect of gastritis and colitis on WAS-evoked c-Fos expression in this area. These data reveal that EE has a region-specific effect on stress-induced c-Fos expression in the corticolimbic system, which is likely to improve stress resilience. The response of the prefrontal cortex - amygdala - hippocampus circuitry to psychological stress is also modified by the systemic stress of gut inflammation, and this interaction between external

  3. c-Fos-related antigens in the central nervous system of an insect, Acheta domesticus. (United States)

    Renucci, M; Tirard, A; Charpin, P; Augier, R; Strambi, A


    Fos-related antigens (Fra) were detected in the nuclei of neurones in young adult Acheta domesticus female crickets by immunohistochemical analysis, using an antibody that recognizes the amino-acid sequence 127-152 of c-Fos protein. Specificity of Fra immunoreactivity was confirmed by Western blot analysis of nuclear extracts from neural tissues. A major immunoreactive doublet with an apparent molecular mass of 52,000/54,000 Da was detected in nuclear extracts. Immunostaining of the 52,000/54,000 Da doublet showed variations in intensity during the first 5 days following the imaginal molt. Staining was more intense between day 2 and day 4 when ecdysteroid titers were high. Expression of Fra was low in allatectomized (i.e., deprived of juvenile hormone and ecdysteroids) and ovariectomized (i.e., deprived of ecdysteroids) females as compared to control females. These results show the involvement of hormone-regulated process in expression of Fra. The effect of nociceptive stimulation on Fra expression was tested. Twenty minutes after removal of the ovipositor, a supplementary band with an apparent molecular mass of 70,000 Da appeared in the nuclear extracts, then decreased and disappeared totally after 45 min. Several other Fos-related antigens with different temporal patterns of expression were also detected.

  4. The time course of systems consolidation of spatial memory from recent to remote retention: A comparison of the Immediate Early Genes Zif268, c-Fos and Arc. (United States)

    Barry, Daniel N; Coogan, Andrew N; Commins, Sean


    Systems consolidation is a process involving the stabilisation of memory traces in the neocortex over time. The medial prefrontal cortex becomes increasingly important during the retrieval of older memories, however the timescale of its involvement is unclear, and the contribution of other neocortical brain regions to remote memory have received little attention. The Immediate Early Genes (IEGs) Zif268, c-Fos and Arc have been utilised as markers of neural activity during spatial memory retrieval, however the lack of a direct comparison between them hinders the interpretation of results. To address these questions, we examined the expression of Zif268, Arc and c-Fos protein in the medial prefrontal cortex, as well as the hippocampus, and the entorhinal, perirhinal, retrosplenial and parietal cortices of male Wistar rats following a probe trial of the Morris water maze either one day, seven days, 14 days or 30 days after acquisition. Activity of the medial prefrontal cortex during retrieval, as measured by all three IEGs, increased in correspondence with the age of the memory, reaching significance between 14 and 30 days. Similar increases in c-Fos and Arc were observed over the course of consolidation in other neocortical and parahippocampal areas, however this pattern was not observed with Zif268. Activity of the hippocampus remained largely unchanged across retention intervals. These findings suggest that systems consolidation of spatial memory takes at least two weeks, are consistent with an ongoing role for the hippocampus in the retrieval of spatial memory, and suggest that c-Fos and Arc may be a more sensitive measure of neural activity in response to behavioural tasks than Zif268. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Systemic L-Kynurenine sulfate administration disrupts object recognition memory, alters open field behavior and decreases c-Fos immunopositivity in C57Bl/6 mice

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    Dániel eVarga


    Full Text Available L-Kynurenine (L-KYN is a central metabolite of tryptophan degradation through the kynurenine pathway (KP. The systemic administration of L-KYN sulfate (L-KYNs leads to a rapid elevation of the neuroactive KP metabolite kynurenic acid (KYNA. An elevated level of KYNA may have multiple effects on the synaptic transmission, resulting in complex behavioral changes, such as hypoactivity or spatial working memory deficits. These results emerged from studies that focused on rats, after low-dose L-KYNs treatment. However, in several studies neuroprotection was achieved through the administration of high-dose L-KYNs.In the present study, our aim was to investigate whether the systemic administration of a high dose of L-KYNs (300 mg/bwkg; i. p. would produce alterations in behavioral tasks (open field or object recognition in C57Bl/6j mice. To evaluate the changes in neuronal activity after L-KYNs treatment, in a separate group of animals we estimated c-Fos expression levels in the corresponding subcortical brain areas. The L-KYNs treatment did not affect the general ambulatory activity of C57Bl/6j mice, whereas it altered their moving patterns, elevating the movement velocity and resting time. Additionally, it seemed to increase anxiety-like behavior, as peripheral zone preference of the open field arena emerged and the rearing activity was attenuated. The treatment also completely abolished the formation of object recognition memory and resulted in decreases in the number of c-Fos-immunopositive-cells in the dorsal part of the striatum and in the CA1 pyramidal cell layer of the hippocampus.We conclude that a single exposure to L-KYNs leads to behavioral disturbances, which might be related to the altered basal c-Fos protein expression in C57Bl/6j mice.

  6. Changing and shielded magnetic fields suppress c-Fos expression in the navigation circuit: input from the magnetosensory system contributes to the internal representation of space in a subterranean rodent. (United States)

    Burger, Tomás; Lucová, Marcela; Moritz, Regina E; Oelschläger, Helmut H A; Druga, Rastislav; Burda, Hynek; Wiltschko, Wolfgang; Wiltschko, Roswitha; Nemec, Pavel


    The neural substrate subserving magnetoreception and magnetic orientation in mammals is largely unknown. Previous experiments have demonstrated that the processing of magnetic sensory information takes place in the superior colliculus. Here, the effects of magnetic field conditions on neuronal activity in the rodent navigation circuit were assessed by quantifying c-Fos expression. Ansell's mole-rats (Fukomys anselli), a mammalian model to study the mechanisms of magnetic compass orientation, were subjected to natural, periodically changing, and shielded magnetic fields while exploring an unfamiliar circular arena. In the undisturbed local geomagnetic field, the exploration of the novel environment and/or nesting behaviour induced c-Fos expression throughout the head direction system and the entorhinal-hippocampal spatial representation system. This induction was significantly suppressed by exposure to periodically changing and/or shielded magnetic fields; discrete decreases in c-Fos were seen in the dorsal tegmental nucleus, the anterodorsal and the laterodorsal thalamic nuclei, the postsubiculum, the retrosplenial and entorhinal cortices, and the hippocampus. Moreover, in inactive animals, magnetic field intensity manipulation suppressed c-Fos expression in the CA1 and CA3 fields of the hippocampus and the dorsal subiculum, but induced expression in the polymorph layer of the dentate gyrus. These findings suggest that key constituents of the rodent navigation circuit contain populations of neurons responsive to magnetic stimuli. Thus, magnetic information may be integrated with multimodal sensory and motor information into a common spatial representation of allocentric space within this circuit.

  7. Systemic 5-Bromo-2-Deoxyuridine Induces Conditioned Flavor Aversion and C-Fos in the Visceral Neuraxis (United States)

    Kimbrough, Adam; Kwon, Bumsup; Eckel, Lisa A.; Houpt, Thomas A.


    5-bromo-2-deoxyuridine (BrdU) is often used in studies of adult neurogenesis and olfactory learning, but it can also have toxic effects on highly proliferative tissue. We found that pairing Kool-Aid flavors with acute systemic injections of BrdU induced strong conditioned flavor aversions. Intermittent injections during Kool-Aid-glucose…

  8. Intracranial self-stimulation facilitates active-avoidance retention and induces expression of c-Fos and Nurr1 in rat brain memory systems. (United States)

    Aldavert-Vera, Laura; Huguet, Gemma; Costa-Miserachs, David; Ortiz, Sandra Pena de; Kádár, Elisabeth; Morgado-Bernal, Ignacio; Segura-Torres, Pilar


    Intracranial self-stimulation (ICSS), a special form of deep brain stimulation in which subjects self-administered electrical stimulation in brain reward areas as the lateral hypothalamus, facilitates learning and memory in a wide variety of tasks. Assuming that ICSS improves learning and memory increasing the activation of memory-related brain areas, the present work examined whether rats receiving an ICSS treatment immediately after the acquisition session of a two-way active avoidance conditioning (TWAA) show both an improved retention and a pattern of increased c-Fos and Nurr1 protein expression in the amygdala, hippocampus, dorsal striatum and/or lateral hypothalamus. The response of both activity-induced IEGs to ICSS was examined not only as markers of neural activation, but because of their reported role in the neural plasticity occurring during learning and memory formation. Results showed that the TWAA conditioning alone increased the expression of the two analysed IEGs in several hippocampal areas, and TWAA retention increased Nurr1 expression in amygdala. ICSS treatment increased the number of c-Fos and Nurr1 positive cells in almost all the brain regions studied when it was measured 70min, but not 48h, after the stimulation. Post-training ICSS treatment, as expected, facilitated the 48h retention of the conditioning. It is noteworthy that in CA3 conditioning and ICSS separately increased c-Fos expression, but this increasing was greater when both, conditioning and ICSS, were combined. Present results suggest that rapid and transient increased expression of these two synaptic plasticity and memory related IEGs in some hippocampal areas, such as CA3, could mediate the facilitative effects of ICSS on learning and memory consolidation. Published by Elsevier B.V.

  9. c-fos protein expression in apoptotic rat spermatocytes induced by gossypol. (United States)

    Teng, C S


    Proto-oncogene products such as c-fos protein with a molecular weight of 62 kDa have been identified in rat spermatocytes. In this study, cellular levels of c-fos proteins in spermatocyte, either with or without gossypol exposure, were quantitatively detected by Western immunoblot and a computer-controlled Spot-denso-program with an IS-1000 Digital Imaging System. Within 0.5-3.5 h (an average of 2 h) of the addition of gossypol, levels of c-fos proteins fell dramatically. The reduction in c-fos proteins occurred 6 h before the apoptosis of spermatocytes in the presence of gossypol. Four hours after exposure to gossypol, the c-fos protein content was overexpressed. The period of c-fos up-regulation lasted for approximately 8 h. The increase in c-fos protein coincided with a high rate of apoptotic cell death. Morphologic structure of the dying cell was revealed by electron microscopy. These results suggest that spermatocyte apoptosis induced by gossypol correlates with biphasic c-fos protein-mediated apoptosis.

  10. Alternative splicing of c-fos pre-mRNA: contribution of the rates of synthesis and degradation to the copy number of each transcript isoform and detection of a truncated c-Fos immunoreactive species

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    Pueyo Carmen


    Full Text Available Abstract Background Alternative splicing is a widespread mechanism of gene expression regulation. Previous analyses based on conventional RT-PCR reported the presence of an unspliced c-fos transcript in several mammalian systems. Compared to the well-defined knowledge on the alternative splicing of fosB, the physiological relevance of the unspliced c-fos transcript in regulating c-fos expression remains largely unknown. This work aimed to investigate the functional significance of the alternative splicing c-fos pre-mRNA. Results A set of primers was designed to demonstrate that, whereas introns 1 and 2 are regularly spliced from primary c-fos transcript, intron 3 remains unspliced in part of total transcript molecules. Here, the two species are referred to as c-fos-2 (+ intron 3 and spliced c-fos (- intron 3 transcripts. Then, we used a quantitatively rigorous approach based on real-time PCR to provide, for the first time, the actual steady-state copy numbers of the two c-fos transcripts. We tested how the mouse-organ context and mouse-gestational age, the synthesis and turnover rates of the investigated transcripts, and the serum stimulation of quiescent cells modulate their absolute-expression profiles. Intron 3 generates an in-frame premature termination codon that predicts the synthesis of a truncated c-Fos protein. This prediction was evaluated by immunoaffinity chromatography purification of c-Fos proteins. Conclusion We demonstrate that: (i The c-fos-2 transcript is ubiquitously synthesized either in vivo or in vitro, in amounts that are higher or similar to those of mRNAs coding for other Fos family members, like FosB, ΔFosB, Fra-1 or Fra-2. (ii Intron 3 confers to c-fos-2 an outstanding destabilizing effect of about 6-fold. (iii Major determinant of c-fos-2 steady-state levels in cultured cells is its remarkably high rate of synthesis. (iv Rapid changes in the synthesis and/or degradation rates of both c-fos transcripts in serum

  11. Repeated toluene exposure increases c-Fos in catecholaminergic cells of the nucleus accumbens shell. (United States)

    Tomaszycki, Michelle L; Aulerich, Kelsey E; Bowen, Scott E


    Toluene is a frequently abused solvent. Previous studies have suggested that toluene acts like other drugs of abuse, specifically on the dopaminergic system in the nucleus accumbens (NAc) and ventral tegmental area (VTA) of the mesolimbic pathway. Although changes in dopamine (DA) levels and c-Fos have been observed in both acute and repeated exposure paradigms, the extent to which c-Fos is localized to catecholaminergic cells is unknown. The present study tested the effects of repeated toluene exposure (1000-4000ppm) on locomotor activity and cells containing c-Fos, tyrosine hydroxylase (TH), or both in the core and shell of the NAc, as well as the anterior and posterior VTA. We focused our study on adolescents, since adolescence is a time of great neural change and a time when individuals tend to be more susceptible to drug abuse. In early tests, toluene dose-dependently increased locomotor activity. Repeated exposure to the highest concentration of toluene resulted in sensitization to toluene's effects on locomotor activity. Although the number of cells immunopositive for c-Fos or TH did not significantly differ across groups, cells immunopositive for TH+c-Fos were higher in the NAc shell of animals exposed to 4000ppm than in animals exposed to air (control) or 1000ppm. Taken together, these findings demonstrate that repeated high dose toluene exposure increases locomotor activity as well as activation of catecholaminergic cells in the shell of the NAc. © 2013 Elsevier Inc. All rights reserved.

  12. Exposure to an open-field arena increases c-Fos expression in a distributed anxiety-related system projecting to the basolateral amygdaloid complex

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    Hale, M.W.; Hay-Schmidt, A.; Mikkelsen, J.D.


    Anxiety states and anxiety-related behaviors appear to be regulated by a distributed and highly interconnected system of brain structures including the basolateral amygdala. Our previous studies demonstrate that exposure of rats to an open-field in high- and low-light conditions results in a marked...... of specific afferent input to this region of the amygdala. In order to identify candidate brain regions mediating anxiety-induced activation of the basolateral amygdaloid complex in rats, we used cholera toxin B subunit (CTb) as a retrograde tracer to identify neurons with direct afferent projections...

  13. Activation of the CREB/c-Fos Pathway during Long-Term Synaptic Plasticity in the Cerebellum Granular Layer (United States)

    Gandolfi, Daniela; Cerri, Silvia; Mapelli, Jonathan; Polimeni, Mariarosa; Tritto, Simona; Fuzzati-Armentero, Marie-Therese; Bigiani, Albertino; Blandini, Fabio; Mapelli, Lisa; D’Angelo, Egidio


    The induction of long-term potentiation and depression (LTP and LTD) is thought to trigger gene expression and protein synthesis, leading to consolidation of synaptic and neuronal changes. However, while LTP and LTD have been proposed to play important roles for sensori-motor learning in the cerebellum granular layer, their association with these mechanisms remained unclear. Here, we have investigated phosphorylation of the cAMP-responsive element binding protein (CREB) and activation of the immediate early gene c-Fos pathway following the induction of synaptic plasticity by theta-burst stimulation (TBS) in acute cerebellar slices. LTP and LTD were localized using voltage-sensitive dye imaging (VSDi). At two time points following TBS (15 min and 120 min), corresponding to the early and late phases of plasticity, slices were fixed and processed to evaluate CREB phosphorylation (P-CREB) and c-FOS protein levels, as well as Creb and c-Fos mRNA expression. High levels of P-CREB and Creb/c-Fos were detected before those of c-FOS, as expected if CREB phosphorylation triggered gene expression followed by protein synthesis. No differences between control slices and slices stimulated with TBS were observed in the presence of an N-methyl-D-aspartate receptor (NMDAR) antagonist. Interestingly, activation of the CREB/c-Fos system showed a relevant degree of colocalization with long-term synaptic plasticity. These results show that NMDAR-dependent plasticity at the cerebellum input stage bears about transcriptional and post-transcriptional processes potentially contributing to cerebellar learning and memory consolidation. PMID:28701927

  14. Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection

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    Yan B.


    Full Text Available We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-transfection as a screening method. In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen-activated protein kinase cascade in reporter gene assays. However, Western blots showed that the induction of Elk-1-regulated expression of endogenous c-Fos was not affected by Pim-1. The phosphorylation and activation of neither Erk1/2 nor Elk-1 was influenced by Pim-1. Also, in the gel shift assay, the pattern of endogenous NFkappaB binding to its probe was not changed in any manner by Pim-1. These data indicate that Pim-1 does not regulate the activation of Erk1/2, Elk-1 or NFkappaB. These contrasting results suggest a pitfall of the transient co-transfection reporter assay in analyzing the regulation of transcription factors outside of the chromosome context. It ensures that results from reporter gene expression assay should be verified by study of endogenous gene expression.

  15. Acute phencyclidine administration induces c-Fos-immunoreactivity in interneurons in cortical and subcortical regions. (United States)

    Hervig, Mona E; Thomsen, Morten S; Kalló, Imre; Mikkelsen, Jens D


    Dysfunction of N-Methyl-d-aspartate receptors (NMDARs) is believed to underlie some of the symptoms in schizophrenia, and non-competitive NMDAR antagonists (including phencyclidine (PCP)) are widely used as pharmacological schizophrenia models. Furthermore, mounting evidence suggests that impaired γ-aminobutyric acid (GABA) neurotransmission contributes to the cognitive deficits in schizophrenia. Thus alterations in GABAergic interneurons have been observed in schizophrenia patients and animal models. Acute systemic administration of PCP increases levels of c-Fos in several cortical and subcortical areas, but whether such induction occurs in specific populations of GABAergic interneuron subtypes still remains to be established. We performed an immunohistochemical analysis of the PCP-induced c-Fos-immunoreactivity (IR) in parvalbumin (PV) and calbindin (CB) interneuron subtypes in the cortex and thalamus of rats. A single dose of PCP (10mg/kg, s.c.) significantly increased total number of c-Fos-IR in: (1) the prelimbic, infralimbic, anterior cingulate, ventrolateral orbital, motor, somatosensory and retrosplenial cortices as well as the nucleus accumbens (NAc), field CA1 of the hippocampus (CA1) field of hippocampus and mediodorsal thalamus (MD); (2) PV-IR cells in the ventrolateral orbitofrontal and retrosplenial cortices and CA1 field of hippocampus; and (3) CB-IR cells in the motor cortex. Overall, our data indicate that PCP activates a wide range of cortical and subcortical brain regions and that a substantial part of this activation is present in GABAergic interneurons in certain regions. This suggests that the psychotomimetic effect of PCP may be mediated via GABAergic interneurons. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

  16. Administration of the GABAA receptor antagonist picrotoxin into rat supramammillary nucleus induces c-Fos in reward-related brain structures. Supramammillary picrotoxin and c-Fos expression

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    Shin Rick


    Full Text Available Abstract Background Picrotoxin blocks GABAA receptors, whose activation typically inhibits neuronal firing activity. We recently found that rats learn to selectively self-administer picrotoxin or bicuculline, another GABAA receptor antagonist, into the supramammillary nucleus (SuM, a posterior hypothalamic structure localized anterior to the ventral tegmental area. Other drugs such as nicotine or the excitatory amino acid AMPA are also self-administered into the SuM. The SuM appears to be functionally linked with the mesolimbic dopamine system and is closely connected with other brain structures that are implicated in motivational processes, including the prefrontal cortex, septal area, preoptic area, lateral hypothalamic area and dorsal raphe nucleus. Here, we hypothesized that these brain structures are activated by picrotoxin injections into the SuM. Results Picrotoxin administration into the SuM markedly facilitated locomotion and rearing. Further, it increased c-Fos expression in this region, suggesting blockade of tonic inhibition and thus the disinhibition of local neurons. This manipulation also increased c-Fos expression in structures including the ventral tegmental area, medial shell of the nucleus accumbens, medial prefrontal cortex, septal area, preoptic area, lateral hypothalamic area and dorsal raphe nucleus. Conclusions Picrotoxin administration into the SuM appears to disinhibit local neurons and recruits activation of brain structures associated with motivational processes, including the mesolimbic dopamine system, prefrontal cortex, septal area, preoptic area, lateral hypothalamic area and dorsal raphe nucleus. These regions may be involved in mediating positive motivational effects triggered by intra-SuM picrotoxin.

  17. Expression of c-Fos in the parabrachial nucleus following peripheral nerve injury in rats. (United States)

    Jergova, Stanislava; Kolesar, Dalibor; Cizkova, Dasa


    Chronic constriction injury (CCI) of the sciatic nerve in rats evokes c-Fos expression at spinal cord level. Using immunohistochemical methods we studied changes in c-Fos expression in the brain stem area, which is suggested as one of the major targets of projection neurons in the superficial dorsal horn laminae, i.e., the parabrachial area. During the first week following injury, the animals developed tactile allodynia. At this time we found an increase of c-Fos positive neurons in the parabrachial area, mainly in the pontine part where the group of c-Fos immunoreactive neurons was present in the dorsal part of lateral parabrachial subnuclei. The number of c-Fos positive neurons gradually decreased up to 14 days following CCI. The specific activation of brain stem neurons during onset of mechanical allodynia could underlie the changes in central nociceptive processing following peripheral nerve injury.

  18. Functional magnetic resonance imaging and c-Fos mapping in rats following an anorectic dose of m-chlorophenylpiperazine. (United States)

    Stark, Jennifer A; Davies, Karen E; Williams, Steve R; Luckman, Simon M


    We have used blood-oxygenation-level-dependent (BOLD) contrast functional magnetic resonance imaging (fMRI) to characterise brain regions responsive to a regulator of appetite. An anorectic dose of the 5-HT(1B/2C) receptor agonist m-chlorophenylpiperazine (mCPP; 3 mg/kg s.c.) was used to compare BOLD contrast fMRI with expression of the c-Fos protein. mCPP was administered to rats, which were then anaesthetised and perfused with fixative 90 min later to allow immunohistochemistry. In a separate experiment, rats were imaged using a T(2)*-weighted gradient echo in a 7 T magnet for 70 min under alpha-chloralose anaesthesia. Both methods detected positive activation in areas of the limbic system: cingulate and orbitofrontal cortices, nucleus accumbens, paraventricular and dorsomedial regions of the hypothalamus. fMRI detected increased signal in the pontine nuclei, the hippocampal formation and olfactory cortex, areas that did not display c-Fos. In addition, BOLD signal was diminished in the ventral tegmental area, preoptic area and the cerebellum-presumably due to decreased neuronal signalling and, therefore, unlikely to display c-Fos. Activity in the limbic system may reflect the appetitive agonist activity of mCPP at the 5-HT(2C) receptor. We conclude that c-Fos provides excellent spatial information but is less useful for detecting inhibited regions, whereas fMRI provides greater temporal resolution. Thus, the two methodologies provide complementary details of brain activity following pharmacological challenge.

  19. Recombinant CART peptide induces c-Fos expression in central areas involved in control of feeding behaviour

    DEFF Research Database (Denmark)

    Vrang, Niels; Tang-Christensen, M.; Larsen, Philip J.


    Regulation of food intake, c-Fos induction, i.c.v. injection, paraventricular nucleus of the hypothalamus......Regulation of food intake, c-Fos induction, i.c.v. injection, paraventricular nucleus of the hypothalamus...

  20. c-Fos induction by a 14T magnetic field in visceral and vestibular relays of the female rat brainstem is modulated by estradiol (United States)

    Cason, Angie M.; Kwon, Bumsup; Smith, James C.; Houpt, Thomas A.


    There is increasing evidence that high magnetic fields interact with the vestibular system of humans and rodents. In rats, exposure to high magnetic fields of 7T or above induces locomotor circling and leads to a conditioned taste aversion if paired with a novel taste. Sex differences in the behavioral responses to magnetic field exposure have been found, such that female rats show more locomotor circling and enhanced conditioned taste aversion compared to male rats. To determine if estrogen modulates the neural response to high magnetic fields, c-Fos expression after 14T magnetic field exposure was compared in ovariectomized rats and ovariectomized rats with estradiol replacement. Compared to sham exposure, magnetic field exposure induced significantly more c-Fos positive cells in the nucleus of the solitary tract and the parabrachial, medial vestibular, prepositus, and supragenualis nuclei. Furthermore, there was a significant asymmetry in c-Fos induction between sides of the brainstem in several regions. In ovariectomized rats, there was more c-Fos expressed in the right side compared to left side in the locus coeruleus and parabrachial, superior vestibular, and supragenualis nuclei; less expression in the right compared to left side of the medial vestibular; and no asymmetry in the prepositus nucleus and the nucleus of the solitary tract. Chronic estradiol treatment modulated the neural response in some regions: less c-Fos was induced in the superior vestibular nucleus and locus coeruleus after estradiol replacement; estradiol treatment eliminated the asymmetry of c-Fos expression in the locus coeruleus and supragenualis nucleus, created an asymmetry in the prepositus nucleus and reversed the asymmetry in the parabrachial nucleus. These results suggest that ovarian steroids may mediate sex differences in the behavioral responses to magnetic field exposure at the level of visceral and vestibular nuclei of the brainstem. PMID:20553875

  1. c-Fos induction by a 14 T magnetic field in visceral and vestibular relays of the female rat brainstem is modulated by estradiol. (United States)

    Cason, Angie M; Kwon, Bumsup; Smith, James C; Houpt, Thomas A


    There is increasing evidence that high magnetic fields interact with the vestibular system of humans and rodents. In rats, exposure to high magnetic fields of 7 T or above induces locomotor circling and leads to a conditioned taste aversion if paired with a novel taste. Sex differences in the behavioral responses to magnetic field exposure have been found, such that female rats show more locomotor circling and enhanced conditioned taste aversion compared to male rats. To determine if estrogen modulates the neural response to high magnetic fields, c-Fos expression after 14 T magnetic field exposure was compared in ovariectomized rats and ovariectomized rats with estradiol replacement. Compared to sham exposure, magnetic field exposure induced significantly more c-Fos positive cells in the nucleus of the solitary tract and the parabrachial, medial vestibular, prepositus, and supragenualis nuclei. Furthermore, there was a significant asymmetry in c-Fos induction between sides of the brainstem in several regions. In ovariectomized rats, there was more c-Fos expressed in the right side compared to left side in the locus coeruleus and parabrachial, superior vestibular, and supragenualis nuclei; less expression in the right compared to left side of the medial vestibular; and no asymmetry in the prepositus nucleus and the nucleus of the solitary tract. Chronic estradiol treatment modulated the neural response in some regions: less c-Fos was induced in the superior vestibular nucleus and locus coeruleus after estradiol replacement; estradiol treatment eliminated the asymmetry of c-Fos expression in the locus coeruleus and supragenualis nucleus, created an asymmetry in the prepositus nucleus and reversed the asymmetry in the parabrachial nucleus. These results suggest that ovarian steroids may mediate sex differences in the behavioral responses to magnetic field exposure at the level of visceral and vestibular nuclei of the brainstem. Copyright 2010 Elsevier B.V. All rights

  2. [Expressions of c-Fos and NADPH-d in the related brainstem during vestibular compensation]. (United States)

    Jiang, Zi-Dong; Wen, Yan-Hua


    To study the mechanism of vestibular compensation and to observe the changes of c-Fos and NADPH-d expressions in the brainstem of the vestibular deafferentation rats in static status or following angular acceleration stimulation. Totally 60 SD rats were randomly divided into control group (labyrinthine intact), complete unilateral vestibular deafferentation (UVD) group, simultaneous complete bilateral vestibular deafferentation (BVD) group (n = 20 in each group). Subgroups (n = 10 in each subgroup) were set for static status or following angular acceleration stimulation in each group. Double labeling with histochemistry-immunohistochemistry was performed to observe c-Fos/NADPH-d neurons. No positive c-Fos/NADPH-d expression was observed in the both sides of medial vestibular nucleus (MVN) and prepositus hypoglossi (PrH) of normal rats in static status and BVD rats whether following canal rotation or not. c-Fos/ NADPH-d expression was observed in the ipsilesional MVN and the contralesional PrH of UVD rats. However, c-Fos/NADPH-d were detected in both sides of MVN and PrH in UVD rats and normal rats following angular acceleration stimulation. In the ipsilesional MVN and the contralesional PrH, c-Fos plays an important role in vestibular compensation, in which nitric oxide acts as a key neurotransmitter.

  3. Pattern of c-Fos expression induced by tail suspension test in the mouse brain

    Directory of Open Access Journals (Sweden)

    Kentaro Hiraoka


    Full Text Available The tail suspension test (TST has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part, ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part. The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to

  4. Virulence factors of Staphylococcus aureus induce Erk-MAP kinase activation and c-Fos expression in S9 and 16HBE14o- human airway epithelial cells. (United States)

    Below, Sabine; Konkel, Anne; Zeeck, Cathrin; Müller, Christian; Kohler, Christian; Engelmann, Susanne; Hildebrandt, Jan-Peter


    Part of the innate defense of bronchial epithelia against bacterial colonization is regulated secretion of salt, water, and mucus as well as defensins and cytokines involving MAP kinase activation and alterations in early gene expression. We tested two different types of immortalized human airway epithelial cells (S9, 16HBE14o-) for activation of Erk-type MAP kinases and for expression of c-Fos on treatment with Staphylococcus aureus culture supernatants from the stationary growth phase [optical density (OD)(540 nm) = 10] or with recombinant S. aureus hemolysins A and B (Hla, Hlb). OD10 supernatants activated Erk-type MAP kinases and c-Fos expression in a concentration-dependent manner. Hla induced Erk-type kinase phosphorylation in S9 but not in 16HBE14o- cells. Hlb induced Erk activation in either cell type. Basal and stimulated levels of Erk-type MAP kinase phosphorylation were sensitive to the Mek1 inhibitor PD-98059, indicating that the bacterial products activated the entire signaling cascade that coregulates IL-8 induction and secretion. While c-Fos expression was enhanced by OD10 supernatants, Hla, and Hlb in S9 cells, 16HBE14o- cells responded to OD10 supernatant and Hlb but not to Hla. In S9 cells, PD-98059 suppressed c-Fos upregulation by OD10 supernatant, Hla, or Hlb, indicating that c-Fos expression requires activation of Erk-type MAP kinases. In 16HBE14o- cells, however, c-Fos expression by OD10 supernatant was sensitive to PD-98059, while that induced by Hlb was not. This indicates that ingredients of OD10 supernatants other than Hla or Hlb are activating Erk-type MAP kinases in 16HBE14o- cells and that other intracellular signaling systems apart from Erk-type MAP kinases contribute to Hlb-mediated regulation of c-Fos. Thus interaction of bacterial factors with airway epithelial cells may be highly cell type specific.

  5. Differential induction of c-Fos and phosphorylated ERK by a noxious stimulus after peripheral nerve injury. (United States)

    Tabata, Mitsuyasu; Terayama, Ryuji; Maruhama, Kotaro; Iida, Seiji; Sugimoto, Tomosada


    In this study, we compared induction of c-Fos and phosphorylated extracellular signal-regulated kinase (p-ERK) in the spinal dorsal horn after peripheral nerve injury. We examined the spinal dorsal horn for noxious heat-induced c-Fos and p-ERK protein-like immunoreactive (c-Fos- and p-ERK-IR) neuron profiles after tibial nerve injury. The effect of administration of a MEK 1/2 inhibitor (PD98059) on noxious heat-induced c-Fos expression was also examined after tibial nerve injury. A large number of c-Fos- and p-ERK-IR neuron profiles were induced by noxious heat stimulation to the hindpaw in sham-operated animals. A marked reduction in the number of c-Fos- and p-ERK-IR neuron profiles was observed in the medial 1/3 (tibial territory) of the dorsal horn at 3 and 7 days after nerve injury. Although c-Fos-IR neuron profiles had reappeared by 14 days after injury, the number of p-ERK-IR neuron profiles remained decreased in the tibial territory of the superficial dorsal horn. Double immunofluorescence labeling for c-Fos and p-ERK induced by noxious heat stimulation to the hindpaw at different time points revealed that a large number of c-Fos-IR, but not p-ERK-IR, neuron profiles were distributed in the tibial territory after injury. Although administration of a MEK 1/2 inhibitor to the spinal cord suppressed noxious heat-induced c-Fos expression in the peroneal territory, this treatment did not alter c-Fos induction in the tibial territory after nerve injury. ERK phosphorylation may be involved in c-Fos induction in normal nociceptive responses, but not in exaggerated c-Fos induction after nerve injury.

  6. Etude acoustique du systeme vocalique du chichewa

    African Journals Online (AJOL)

    le yao (10 %), le tumbuka (6,4 %), le nyakyusa-ngonde (2,9 %), le sena. (2,4 %) et le tonga (2, 1 %). Les langues pratiquees par ces .... Tisfyeni tip(1te kwambfri. Laissez-les beaucoup provoquer. Le deuxieme ..... rencontrer Jes cinq phonemes du chichewa : /a e i o ul. Rappelons que Jes elements stables de la phrase ...

  7. Akv murine leukemia virus enhances bone tumorigenesis in hMT-c-fos-LTR transgenic mice

    DEFF Research Database (Denmark)

    Schmidt, Jörg; Krump-Konvalinkova, Vera; Luz, Arne


    hMt-c-fos-LTR transgenic mice (U. Rüther, D. Komitowski, F. R. Schubert, and E. F. Wagner. Oncogene 4, 861–865, 1989) developed bone sarcomas in 20% (3/15) of females at 448 ± 25 days and in 8% (1/12) of males at 523 days. After infection of newborns with Akv, an infectious retrovirus derived from...... the ecotropic provirus of the AKR mouse, 69% (20/28) of female animals and 83% (24/29) of males developed malignant fibrous-osseous tumors. The tumors in infected transgenics developed with higher frequency and a 200-days shorter mean tumor latency period. The hMt-c-fos-LTR transgene was expressed in all...... that Akv exerts distinct pathogenic effects on the skeleton. In hMt-c-fos-LTR transgenic mice, predisposed to bone sarcomagenesis, Akv acts synergistically with the fos transgene, resulting in the development of fibrous-osseous tumors...

  8. Lack of estradiol modulation of sleep deprivation-induced c-Fos in the rat brain. (United States)

    Mashoodh, Rahia; Stamp, Jennifer A; Wilkinson, Michael; Rusak, Benjamin; Semba, Kazue


    Women recover from sleep deprivation more efficiently than men, but the mechanism for this difference is unknown. Effects of estrogen on sleep suggest that it could play a role, but the brain targets on which estrogen may act to have this effect have not been identified. Sleep deprivation increases levels of the immediate-early gene protein c-Fos in selected brain regions, but it is unknown whether estrogen modulates this response. We investigated the influence of different levels of exogenous estradiol on the c-Fos response to sleep deprivation in ovariectomized female rats. Female rats were treated with low or high levels of estradiol (mimicking diestrous and proestrous levels, respectively) delivered via subcutaneous silastic tubes. Control ovariectomized females and sham-operated males were implanted with tubes filled with cholesterol. One week after surgery, half of the rats underwent a 3 h period of sleep deprivation during the light phase in a motorized Wahmann activity wheel that rotated constantly at a slow speed, while half were confined to fixed wheels. Immediately after sleep deprivation, animals were killed and their brains processed to detect c-Fos using immunohistochemistry. Sleep deprivation increased the number of c-Fos positive cells in a number of brain areas, including the caudate putamen, medial preoptic area, perifornical hypothalamus, and anterior paraventricular thalamic nucleus. Other areas, including the suprachiasmatic nucleus, posterior paraventricular hypothalamic nucleus, posterior paraventricular thalamic nucleus, arcuate nucleus, and central amygdala, did not respond to 3 h sleep deprivation with a significant increase in c-Fos levels. Levels of c-Fos induced in the selected brain regions by sleep deprivation were not modulated by estrogen levels, nor by sex.

  9. Fluorescent visualisation of the hypothalamic oxytocin neurones activated by cholecystokinin-8 in rats expressing c-fos-enhanced green fluorescent protein and oxytocin-monomeric red fluorescent protein 1 fusion transgenes. (United States)

    Katoh, A; Shoguchi, K; Matsuoka, H; Yoshimura, M; Ohkubo, J-I; Matsuura, T; Maruyama, T; Ishikura, T; Aritomi, T; Fujihara, H; Hashimoto, H; Suzuki, H; Murphy, D; Ueta, Y


    The up-regulation of c-fos gene expression is widely used as a marker of neuronal activation elicited by various stimuli. Anatomically precise observation of c-fos gene products can be achieved at the RNA level by in situ hybridisation or at the protein level by immunocytochemistry. Both of these methods are time and labour intensive. We have developed a novel transgenic rat system that enables the trivial visualisation of c-fos expression using an enhanced green fluorescent protein (eGFP) tag. These rats express a transgene consisting of c-fos gene regulatory sequences that drive the expression of a c-fos-eGFP fusion protein. In c-fos-eGFP transgenic rats, robust nuclear eGFP fluorescence was observed in osmosensitive brain regions 90 min after i.p. administration of hypertonic saline. Nuclear eGFP fluorescence was also observed in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) 90 min after i.p. administration of cholecystokinin (CCK)-8, which selectively activates oxytocin (OXT)-secreting neurones in the hypothalamus. In double transgenic rats that express c-fos-eGFP and an OXT-monomeric red fluorescent protein 1 (mRFP1) fusion gene, almost all mRFP1-positive neurones in the SON and PVN expressed nuclear eGFP fluorescence 90 min after i.p. administration of CCK-8. It is possible that not only a plane image, but also three-dimensional reconstruction image may identify cytoplasmic vesicles in an activated neurone at the same time. © 2014 British Society for Neuroendocrinology.

  10. Actions of agonists and antagonists of the ghrelin/GHS-R pathway on GH secretion, appetite and cFos activity

    Directory of Open Access Journals (Sweden)

    Rim eHassouna


    Full Text Available The stimulatory effects of ghrelin, a 28-AA acylated peptide originally isolated from stomach, on GH secretion and feeding are exclusively mediated through the growth hormone secretagogue 1a receptor (GHS-R1a, the only ghrelin receptor described so far. Several GHS-R1a agonists and antagonists have been developed to treat metabolic or nutritional disorders but their mechanisms of action in the central nervous system remain poorly understood.In the present study, we compared the activity of BIM-28163, a GHS-R1a antagonist and of several agonists, including native ghrelin and the potent synthetic agonist, BIM-28131, to modulate food intake, GH secretion and c-Fos activity in ArcN, NTS and AP in wild-type and NPY-GFP mice.BIM-28131 was as effective as ghrelin in stimulating GH secretion, but more active than ghrelin in inducing feeding. It stimulated cFos activity similarly to ghrelin in the NTS and AP but was more powerful in the ArcN, suggesting that the super-agonist activity of BIM-28131 is mostly mediated in the ArcN. BIM-28163 antagonized ghrelin-induced GH secretion but not ghrelin-induced food consumption and cFos activation, rather it stimulated food intake and cFos activity without affecting GH secretion. The level of cFos activation was dependent on the region considered: BIM-28163 was as active as ghrelin in the NTS, but less active in the ArcN and AP. All compounds also induced cFos immunoreactivity in ArcN NPY neurons but BIM-28131 was the most active.In conclusion, these data demonstrate that two peptide analogs of ghrelin, BIM-28163 and BIM-28131, are powerful stimulators of appetite in mice, acting through pathways and key brain regions involved in the control of appetite that are only partially superimposable from those activated by ghrelin. A better understanding of the molecular pathways activated by these compounds could be useful in devising future therapeutic applications, such as for cachexia and anorexia.

  11. Spinal distribution of c-Fos activated neurons expressing enkephalin in acute and chronic pain models. (United States)

    Hossaini, Mehdi; Duraku, Liron S; Kohli, Somesh K; Jongen, Joost L M; Holstege, Jan C


    The endogenous opioid enkephalin is known to inhibit spinal nociceptive transmission. Here we investigated activation of spinal enkephalinergic neurons by determining the proportions of c-Fos expressing (activated) spinal neurons that were enkephalinergic after different acute and chronic peripheral nociceptive stimuli. The number of c-Fos-activated neurons in the dorsal horn was increased after hind paw injection of capsaicin, formalin or complete Freund's adjuvant (CFA, 1.5 hrs - 4 days). The numbers of these neurons that were enkephalinergic increased after paraformaldehyde, and at 20 hrs, but not 1.5 hrs or 4 days post-CFA as compared to saline. In the spared nerve injury (SNI) model of neuropathic pain, c-Fos expression was increased acutely (2 hrs) and chronically (2 weeks), and a greater number of these were enkephalinergic in the nerve-injured animals acutely compared to controls (sham-SNI). Combining all acute (=2 hrs) versus chronic (≥20 hrs) treatment groups, there was a significant decrease in the percentage of activated neurons that were enkephalinergic in superficial layers, but a significant increase in the deeper layers of the dorsal horn in the chronic treatment group. It is concluded that the overall percentage of c-Fos activated neurons that contained enkephalin was not significantly different between acute and chronic pain phases. However, the shift in localization of these neurons within the spinal dorsal horn indicates a noxious stimulus directed activation pattern. © 2013 Published by Elsevier B.V.

  12. Neuroendocrine and behavioral responses and brain pattern of c-fos induction associated with audiogenic stress. (United States)

    Campeau, S; Watson, S J


    The present study determined simultaneously the behavioural, neuroendocrine and regional brain activity, using semi-quantitative analysis of c-fos mRNA induction, produced by 30 min of auditory stimulation at different white noise intensities (background 60 dB, 70, 80, 90 and 105 dBA), in rats. Only the highest noise intensities (90 and 105 dB) significantly increased corticosterone release after 30 min stimulation. Behaviourally, the 105 dB noise condition reliably reduced overall activity, and moderate noise intensities (70 and 80 dB) increased sleeping time. Three distinct patterns of c-fos mRNA induction were observed. First, following exposure to the experimental cages, a wide pattern of brain activation was obtained in experimental animals irrespective of noise intensity presentation, compared to the naive rats. Second, a number of auditory structures (cochlear nuclei, superior olivary complex, nuclei of the lateral lemniscus, inferior colliculus and the medial division of the medial geniculate body) displayed a clear intensity-dependent increase in c-fos induction. Third, compared to all other conditions, the stressed rats (90 and 105 dB conditions) displayed significantly higher c-fos induction in relatively few areas. Particularly intense c-fos induction was observed in the bed nucleus of the stria terminalis, especially its anterior medial and ventral aspects, the septohypothalamic nucleus, the ventral lateral septum, the ventral portion of the dentate gyrus, a number of hypothalamic nuclei including the lateral preoptic area, the medial preoptic nucleus and the paraventricular nucleus, the median raphe and the pedunculopontine tegmental nucleus. The involvement of a number of these structures in a specific audiogenic stress responsive circuit is discussed.

  13. Repeated forced swim stress enhances CFA-evoked thermal hyperalgesia and affects the expressions of pCREB and c-Fos in the insular cortex. (United States)

    Imbe, H; Kimura, A; Donishi, T; Kaneoke, Y


    Stress affects brain activity and promotes long-term changes in multiple neural systems. Exposure to stressors causes substantial effects on the perception and response to pain. In several animal models, chronic stress produces lasting hyperalgesia. The insular (IC) and anterior cingulate cortices (ACC) are the regions exhibiting most reliable pain-related activity. And the IC and ACC play an important role in pain modulation via the descending pain modulatory system. In the present study we examined the expression of phospho-cAMP response element-binding protein (pCREB) and c-Fos in the IC and ACC after forced swim stress (FS) and complete Freund's adjuvant (CFA) injection to clarify changes in the cerebral cortices that affect the activity of the descending pain modulatory system in the rats with stress-induced hyperalgesia. FS (day 1, 10min; days 2-3, 20min) induced an increase in the expression of pCREB and c-Fos in the anterior IC (AIC). CFA injection into the hindpaw after the FS shows significantly enhanced thermal hyperalgesia and induced a decrease in the expression of c-Fos in the AIC and the posterior IC (PIC). Quantitative image analysis showed that the numbers of c-Fos-immunoreactive neurons in the left AIC and PIC were significantly lower in the FS+CFA group (L AIC, 95.9±6.8; L PIC, 181.9±23.1) than those in the naive group (L AIC, 151.1±19.3, pCFA-induced thermal hyperalgesia through dysfunction of the descending pain modulatory system. Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

  14. Acute restraint stress decreases c-fos immunoreactivity in hilar mossy cells of the adult dentate gyrus (United States)

    Moretto, Jillian N.; Duffy, Áine M.


    Although a great deal of information is available about the circuitry of the mossy cells (MCs) of the dentate gyrus (DG) hilus, their activity in vivo is not clear. The immediate early gene c-fos can be used to gain insight into the activity of MCs in vivo, because c-fos protein expression reflects increased neuronal activity. In prior work, it was identified that control rats that were perfusion-fixed after removal from their home cage exhibited c-fos immunoreactivity (ir) in the DG in a spatially stereotyped pattern: ventral MCs and dorsal granule cells (GCs) expressed c-fos protein (Duffy et al., Hippocampus 23:649–655, 2013). In this study, we hypothesized that restraint stress would alter c-fos-ir, because MCs express glucocorticoid type 2 receptors and the DG is considered to be involved in behaviors related to stress or anxiety. We show that acute restraint using a transparent nose cone for just 10 min led to reduced c-fos-ir in ventral MCs compared to control rats. In these comparisons, c-fos-ir was evaluated 30 min after the 10 min-long period of restraint, and if evaluation was later than 30 min c-fos-ir was no longer suppressed. Granule cells (GCs) also showed suppressed c-fos-ir after acute restraint, but it was different than MCs, because the suppression persisted for over 30 min after the restraint. We conclude that c-fos protein expression is rapidly and transiently reduced in ventral hilar MCs after a brief period of restraint, and suppressed longer in dorsal GCs. PMID:28190104

  15. β-glucan reduces exercise-induced stress through downregulation of c-Fos and c-Jun expression in the brains of exhausted rats. (United States)

    Hong, Heeok; Kim, Chang-Ju; Kim, Jae-Deung; Seo, Jin-Hee


    Immediate-early genes are involved in acute stress responses in the central nervous system. β-glucan stimulates innate immune defenses, exerts an anti-tumor response and increases resistance to a wide variety of types of infection. To date, the effect of β-glucan on the expression of immediate-early genes under stressful conditions has not been elucidated. In the present study, the effects of β-glucan on the expression of the oncogenes c-Fos and c-Jun in the hypothalamus, dentate gyrus and dorsal raphe in rats following exhaustive treadmill running were investigated. Male Sprague Dawley rats were randomly divided into five groups (n=10 in each group) as follows: Control, exercise, exercise and 50 mg/kg β-glucan treatment, exercise and 100 mg/kg β-glucan treatment, and exercise and 200 mg/kg β-glucan treatment. Rats in the β-glucan‑treated groups were administered β-glucan at the respective dose once per day for seven days. Rats in the exercise groups performed treadmill running once per day for six days. On the seventh day of the experiment, the time to exhaustion in response to treadmill running was determined for the exercise groups. The expression of c-Fos and c-Jun in the hypothalamus, dorsal raphe and hippocampus was enhanced by exhaustive treadmill running. Administration of β-glucan resulted in an increase in the time to exhaustion and the suppression of the exercise-induced increment in c-Fos and c-Jun expression. In conclusion, β-glucan may exert an alleviating effect on exercise-induced stress through the suppression of c-Fos and c-Jun expression in the brains of exhausted rats.

  16. Control of c-fos and c-myc proto-oncogene induction in rat thyroid cells in culture

    Energy Technology Data Exchange (ETDEWEB)

    Isozaki, O.; Kohn, L.D. (National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD (USA))


    Removal of TSH, insulin, and cortisol from the medium, and decreasing the serum content to 0.2%, abolishes both the proliferate and differentiated state of FRTL-5 rat thyroid cells in culture. In these basal conditions, the individual addition of TSH, insulin, insulin-like growth factor-I (IGF-I), phorbol 12-myristate 13-acetate (TPA), alpha 1-adrenergic agents, or A23187, increase c-myc and/or c-fos proto-oncogene expression. Under the same conditions, only the addition of TSH increased cAMP levels; 8-bromo-cAMP can increase c-myc or c-fos mRNA levels. Pretreatment of cells with phorbol 12,13-dibutyrate, an agent which down regulates the C-kinase, completely inhibits the effect of TPA on proto-oncogene expression but has no affect on the A23187 induced-increase. The sum of these results indicate that at least four separate signal systems independently increase c-myc or c-fos gene expression in FRTL-5 cells cAMP (TSH), C-kinase (TPA), Ca++/phosphoinositide (A23187), and that influenced by insulin/IGF-I. None of the ligands, when individually returned to cells in basal medium (no TSH, insulin, or cortisol and only 0.2% serum), increases cell number; norepinephrine, and A23187 do not increase (3H)thymidine incorporation into DNA under these conditions; and combinations of the ligands can be more than additive in effecting (3H)thymidine incorporation into DNA but are only additive in effecting proto-oncogene expression. Insulin/IGF-I plus TSH or insulin/IGF-I plus norepinephrine can increase both proto-oncogene expression and (3H)thymidine incorporation into DNA to the same extent; however, the former combination can increase cell number whereas the latter cannot. There is therefore no simple correlation between the ability of the above ligands to increase proto-oncogene expression and their ability to increase cell number or induce DNA synthesis.

  17. Cloning, structural organization, and chromosomal assignment of the porcine c-fos proto-oncogene, FOS. (United States)

    Reiner, G; Heinricy, L; Brenig, B; Geldermann, H; Dzapo, V


    The complete porcine c-fos proto-oncogene (FOS) with flanking regions was cloned and sequenced. FOS consists of four exons at amino acids 1-47, 48-131, 132-167, and 168-380 and includes all the typical motifs of the fos proto-oncogene. The promoter contains consensus sequences for CRE, SRE, CaRE, and the E-Box, as well as an AP-1 site. Homologies between human and swine were between 89.7% and 96.3% in the exons. Based on somatic cell hybrid panel screening and known homologies between swine chromosome 7 and human chromosome 14, the porcine c-fos gene was assigned to chromosome 7q23. Copyright 2000 S. Karger AG, Basel.

  18. Forced expression of stabilized c-Fos in dendritic cells reduces cytokine production and immune responses in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Ryoko; Suzuki, Mayu; Sakaguchi, Ryota; Hasegawa, Eiichi; Kimura, Akihiro; Shichita, Takashi; Sekiya, Takashi [Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582 (Japan); Japan Science and Technology Agency, CREST, Chiyoda-ku 102-0075 (Japan); Shiraishi, Hiroshi [Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, Saga (Japan); Shimoda, Kouji [Department of Laboratory Animal Center, Keio University School of Medicine, Tokyo (Japan); Yoshimura, Akihiko, E-mail: [Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582 (Japan); Japan Science and Technology Agency, CREST, Chiyoda-ku 102-0075 (Japan)


    Highlights: Black-Right-Pointing-Pointer Dendritic cells expressing stabilized c-Fos produced less inflammatory cytokines. Black-Right-Pointing-Pointer Dendritic cells expressing stabilized c-Fos activated T cells less efficiently. Black-Right-Pointing-Pointer Transgenic mice expressing stabilized c-Fos were resistant to EAE model. -- Abstract: Intracellular cyclic adenosine monophosphate (cAMP) suppresses innate immunity by inhibiting proinflammatory cytokine production by monocytic cells. We have shown that the transcription factor c-Fos is responsible for cAMP-mediated suppression of inflammatory cytokine production, and that c-Fos protein is stabilized by IKK{beta}-mediated phosphorylation. We found that S308 is one of the major phosphorylation sites, and that the S308D mutation prolongs c-Fos halflife. To investigate the role of stabilized c-Fos protein in dendritic cells (DCs) in vivo, we generated CD11c-promoter-deriven c-FosS308D transgenic mice. As expected, bone marrow-derived DCs (BMDCs) from these Tg mice produced smaller amounts of inflammatory cytokines, including TNF-{alpha}, IL-12, and IL-23, but higher levels of IL-10, in response to LPS, than those from wild-type (Wt) mice. When T cells were co-cultured with BMDCs from Tg mice, production of Th1 and Th17 cytokines was reduced, although T cell proliferation was not affected. Tg mice demonstrated more resistance to experimental autoimmune encephalomyelitis (EAE) than did Wt mice. These data suggest that c-Fos in DCs plays a suppressive role in certain innate and adaptive immune responses.

  19. C-fos protein expression in Spitz nevi, common melanocytic nevi, and malignant melanomas. (United States)

    Bergman, R; Kerner, H; Manov, L; Friedman-Birnbaum, R


    The expression of c-fos protein was studied in formalin-fixed, paraffin-embedded sections of 11 compound Spitz nevi (SNs), 16 ordinary compound melanocytic nevi (MNs), and 17 malignant melanomas (MMs) using monoclonal antibody MAB1283 and an immunoperoxidase technique. Eleven (100%) SNs, 15 (94%) MNs, and 16 (94%) MMs showed positive reactions in some of the tumor cells (p = nonsignificant). In the majority of the tumors the staining was located in nuclei and graded as moderate to strong in intensity. The percentages of positively stained cells did not differentiate the three types of tumor, although they were higher in the melanocytic nevi. Most of the lesions with a significant dermal component did not show stratification of staining with progressive descent into the dermis. Positive staining for c-fos was also frequently found in the normal skin constituents within and adjacent to the melanocytic tumors. In conclusion, the pattern of expression of c-fos in routinely processed specimens does not differentiate between SNs, MNs, and MMs.

  20. c-Fos immunoreactivity of neural cells in intoxication due to high-dose methamphetamine. (United States)

    Tomita, Masafumi; Katsuyama, Hironobu; Watanabe, Yoko; Shibaike, Yoshinori; Yoshinari, Haruhiko; Tee, Jia Wei; Iwachidou, Nobuhisa; Miyamoto, Osamu


    Methamphetamine (METH) is a powerful and toxic psychostimulant that is abused worldwide. Although many studies of its toxic functions have been done on animals and humans, the mechanism is still poorly understood. In addition, the doses of METH examined have often been low. Here, we investigated the effects of intoxication due to administration of 20 mg/kg METH on neuronal activity. The mice showed hyperthermia and stereotyped behavior during 60 min after injection. We examined plasma stress hormone levels, which indicated that exposure to METH stimulated the hypothalamic-pituitary-adrenal (HPA) axis and caused release of stress hormones soon after injection. The maximum levels of adrenocorticotropic hormone and corticosterone occurred 10 and 60 min, respectively, after injection. We examined c-Fos protein in 16 different brain regions at 60 min post injection to identify potential brain regions subject to the stimulant effect. Nine regions, including the anterior hypothalamic area, medial preoptic area, lateral hypothalamic area, paraventricular thalamic nucleus, lateral anterior hypothalamic nucleus, lateral septum, striatum, nucleus accumbens, and amygdala, showed a significant increase in c-Fos expression, while the other seven regions did not. These results indicate that responsive neurons in the regions containing c-Fos immunoreactivity (Fos-IR) may undergo cellular reaction to high-dose METH administration. The present study provides support for a relationship among hyperthermia, the HPA axis and neuronal activities in limited brain regions on exposure to 20 mg/kg METH.

  1. c-Fos is involved in inhibition of human bladder carcinoma T24 cells by brazilin. (United States)

    Zhang, Tingting; Fan, Xinping; Song, Lili; Ren, Lu; Ma, Enbo; Zhang, Shengwan; Ren, Liansheng; Zheng, Yaowu; Zhang, Jianzhen


    Crude brazilin extract from Sappan wood has demonstrated strong anti tumor activity in the mouse model of human bladder carcinoma and clinical trial for intravesical therapy. Purified brazilin was confirmed the most active molecule in inhibition of bladder carcinoma T24 cells. Brazilin decreased proliferation and viability of T24 cells in a dose- and time-dependent manner, with a calculated LC50 of 32 µg/mL. More than 1,000 of genes were found upregulated and down regulated by brazilin treatment in digital gene expression profiling. Gene ontology analysis indicated that stress response, apoptosis, and cell cycle regulatory pathways were highly enriched. Among the regulated genes, c-Fos was the most and specifically upregulated. Overexpression of c-Fos in T24 cells resulted in tumor cell specific changes in cell morphology and viability. Over expression of stress-responsive gene, HSP70, and other highly upregulated genes did not have any effect on cell growth. Brazilin may inhibit T24 cell growth and trigger cell death through a c-Fos-mediated and tumor cell specific signaling pathway. Further studies of its down stream mediators may help to identify better tumor cell type specific drug targets. © 2015 International Union of Biochemistry and Molecular Biology.

  2. Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin

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    Lipigorngoson Suwiwek


    Full Text Available Abstract Background We investigated the chemopreventive action of dietary curcumin on 7,12-dimethylbenz(aanthracene (DMBA-initiated and 12,0-tetradecanoylphorbol-13-acetate (TPA-promoted skin tumor formation in Swiss albino mice. Curcumin, a yellow coloring matter isolated from roots of Curcuma longa Linn, is a phenolic compound possessing antioxidant, free radical scavenger, and antiinflammatory properties. It has been shown by previously reported work that TPA-induced skin tumors were inhibited by topical application of curcumin, and curcumin has been shown to inhibit a variety of biological activities of TPA. Topical application of curcumin was reported to inhibit TPA-induced c-fos, c-jun and c-myc gene expression in mouse skin. This paper reports the effects of orally administered curcumin, which was consumed as a dietary component at concentrations of 0.2 % or 1 %, in ad libitum feeding. Results Animals in which tumors had been initiated with DMBA and promoted with TPA experienced significantly fewer tumors and less tumor volume if they ingested either 0.2% or 1% curcumin diets. Also, the dietary consumption of curcumin resulted in a significantly decreased expression of ras and fos proto-oncogenes in the tumorous skin, as measured by enhanced chemiluminesence Western blotting detection system (Amersham. Conclusions Whereas earlier work demonstrated that topical application of curcumin to mouse skin inhibited TPA-induced expression of c-fos, c-jun and c-myc oncogenes, our results are the first to show that orally consumed curcumin significantly inhibited DMBA- and TPA-induced ras and fos gene expression in mouse skin.

  3. c-fos is induced in the hippocampus during consolidation of sexual imprinting in the zebra finch (Taeniopygia guttata). (United States)

    Sadananda, Monika; Bischof, Hans-Joachim


    c-fos was used to mark regions of enhanced neuronal activity during sexual imprinting, an early learning process by which information about the prospective sexual partner is acquired and consolidated. In the present study, we demonstrate that the hippocampus, already known for its specialized spatial memory capacities in navigating pigeons and in food-storing birds, depicts a selective differential c-fos induction in a situation shown to lead to sexual imprinting, that is, exposing previously isolated male birds to a female for 1 h. c-fos induction is lateralized, the left hippocampus showing more c-fos activity than the right. Our results would indicate a role for the hippocampus in the consolidation process of imprinting, probably in the transfer of information to the other telencephalic areas that show alterations in synaptic connectivity as a result of consolidation of sexual imprinting.

  4. Cryptotanshinone Regulates Androgen Synthesis through the ERK/c-Fos/CYP17 Pathway in Porcine Granulosa Cells

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    Danfeng Ye


    Full Text Available The aim of the study is to investigate the molecular mechanism behind androgen reduction in porcine granulosa cells (pGCs with Salvia miltiorrhiza Bunge extract cryptotanshinone. PGCs were isolated from porcine ovaries and identified. Androgen excess model of the pGCs was induced with the MAPK inhibitor PD98059 and then treated with cryptotanshinone. The testosterone level was measured by radioimmunoassay in the culture media. The protein levels of P-ERK1/2, c-Fos, and CYP17 in the cells were measured by western blot. Cryptotanshinone decreased the concentration of testosterone and the protein level of CYP17 and increased the protein levels of P-ERK1/2 and c-Fos in the androgen excess mode. After the c-Fos gene was silenced by infection with c-Fos shRNA lentivirus, we measured the mRNA expression by quantitative RT-PCR and protein level by western blot of P-ERK1/2, c-Fos, and CYP17. This showed that the mRNA expression and protein level of P-ERK1/2 and c-Fos were significantly reduced in the shRNA–c-Fos group compared to the scrambled group, while those of CYP17 were significantly increased. So we concluded that cryptotanshinone can significantly reduce the androgen excess induced by PD98059 in pGCs. The possible molecular mechanism for this activity is regulating the ERK/c-Fos/CYP17 pathway.

  5. c-Fos and Dusp1 confer non-oncogene addiction in BCR-ABL induced leukemia (United States)

    Kesarwani, Meenu; Kincaid, Zachary; Gomaa, Ahmed; Huber, Erika; Rohrabaugh, Sara; Siddiqui, Zain; Bouso, Muhammad F.; Latif, Tahir; Xu, Ming; Komurov, Kakajan; Mulloy, James C.; Cancelas, Jose A.; Grimes, H. Leighton; Azam, Mohammad


    Tyrosine kinase inhibitor (TKI) therapy for human cancers is not curative, with relapse due to the continuing presence of tumor cells, referred to as minimal residual disease (MRD) cells. MRD stem or progenitor cells survival in the absence of oncogenic kinase signaling, a phenomenon referred to as intrinsic resistance, depends on diverse growth factors. Here, we report that oncogenic kinase and growth factor signaling converge to induce the expression of the signaling proteins c-Fos and Dusp1. Genetic deletion of c-Fos and Dusp1 suppressed tumor growth in a BCR-ABL-induced mouse model of chronic myeloid leukemia (CML). Pharmacological inhibition of c-Fos, Dusp1 and BCR-ABL eradicated MRD in multiple in vivo models, as well as in primary CML patient xenotransplanted mice. Growth factor signaling also conferred TKI resistance and induced c-FOS and DUSP1 expression in tumor cells modeling other types of kinase-driven leukemias. Our data demonstrate that c-Fos and Dusp1 expression levels determine the threshold of TKI efficacy, such that growth factor-induced expression of c-Fos and Dusp1 confers intrinsic resistance to TKI therapy in a wide-ranging set of leukemias, and may represent a unifying Achilles heel of kinase-driven cancers. PMID:28319094

  6. Prenatal valproic acid exposure disrupts tonotopic c-Fos expression in the rat brainstem. (United States)

    Dubiel, A; Kulesza, R J


    Autism spectrum disorder (ASD) is a group of neurodevelopmental conditions characterized by difficulties in communication and social interactions, restricted, repetitive behaviors and sensory abnormalities. Notably, the vast majority of individuals with ASD experience some degree of auditory dysfunction and we have recently reported consistent hypoplasia and dysmorphology in auditory brainstem centers in individuals with ASD. Prenatal exposure to the antiepileptic drug valproic acid (VPA) is associated with an increased risk of ASD. In rodents, prenatal exposure to VPA is employed as an animal model of ASD and is associated with a number of anatomical, physiological and behavioral deficits, including hypoplasia and dysmorphology of auditory brainstem centers. Based on these observations, we hypothesized that such dysmorphology in VPA-exposed animals would translate into abnormal neuronal activity in brainstem circuits and irregular tonotopic maps. Herein, we have subjected control and VPA-exposed animals to 4- or 16-kHz tones and examined neuronal activation with immunohistochemistry for c-Fos. After these exposures, we identified significantly more c-Fos-positive neurons in the auditory brainstem of VPA-exposed animals. Additionally, we observed a larger dispersion of c-Fos-positive neurons and shifted tonotopic bands in VPA-exposed rats. We interpret these findings to suggest hyper-responsiveness to sounds and disrupted mapping of sound frequencies after prenatal VPA exposure. Based on these findings, we suggest that such abnormal patterns of activation may play a role in auditory processing deficits in ASD. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

  7. c-fos/c-jun expression and AP-1 activation in skin fibroblasts from centenarians. (United States)

    Grassilli, E; Bellesia, E; Salomoni, P; Croce, M A; Sikora, E; Radziszewska, E; Tesco, G; Vergelli, M; Latorraca, S; Barbieri, D; Fagiolo, U; Santacaterina, S; Amaducci, L; Tiozzo, R; Sorbi, S; Franceschi, C


    In vitro replicative senescence is characterized by an irreversible growth arrest due to the inability of the cell to induce some key regulators of cell cycle progression, such as c-fos and AP-1, in response to mitogenic stimuli. In vitro replicative senescence and in vivo aging have been assumed to be two related phenomena, likely controlled by overlapping or interacting genes. As a corollary, fibroblasts from centenarians, which have undergone a long process of senescence in vivo should have very limited proliferative capability. On the contrary, in a previous work we found that fibroblasts from centenarians exhibited the same capacity to respond to different mitogenic stimuli as fibroblasts from young donors. Here we provide evidences that the well preserved proliferative response is likely due to the fact that some pivotal regulators- c-fos, c-jun and AP-1-are still fully inducible, despite a long process of in vivo senescence. Our data therefore suggest that in vivo and in vitro aging are separate phenomena whose possible relationships, if any, have to be ascertained very carefully.

  8. Male song quality modulates c-Fos expression in the auditory forebrain of the female canary. (United States)

    Monbureau, Marie; Barker, Jennifer M; Leboucher, Gérard; Balthazart, Jacques


    In canaries, specific phrases of male song (sexy songs, SS) that are difficult to produce are especially attractive for females. Females exposed to SS produce more copulation displays and deposit more testosterone into their eggs than females exposed to non-sexy songs (NS). Increased expression of the immediate early genes c-Fos or zenk (a.k.a. egr-1) has been observed in the auditory forebrain of female songbirds hearing attractive songs. C-Fos immunoreactive (Fos-ir) cell numbers were quantified here in the brain of female canaries that had been collected 30min after they had been exposed for 60min to the playback of SS or NS or control white noise. Fos-ir cell numbers increased in the caudomedial mesopallium (CMM) and caudomedial nidopallium (NCM) of SS birds as compared to controls. Song playback (pooled SS and NS) also tended to increase average Fos-ir cell numbers in the mediobasal hypothalamus (MBH) but this effect did not reach full statistical significance. At the individual level, Fos expression in CMM was correlated with its expression in NCM and in MBH but also with the frequency of calls that females produced in response to the playbacks. These data thus indicate that male songs of different qualities induce a differential metabolic activation of NCM and CMM. The correlation between activation of auditory regions and of the MBH might reflect the link between auditory stimulation and changes in behavior and reproductive physiology. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Downsizing Proto-oncogene cFos to Short Helix-Constrained Peptides That Bind Jun. (United States)

    Baxter, Daniel; Perry, Samuel R; Hill, Timothy A; Kok, W Mei; Zaccai, Nathan R; Brady, R Leo; Fairlie, David P; Mason, Jody M


    The oncogenic transcription factor activator protein-1 (AP-1) is a DNA-binding protein that assembles through dimerization of Fos and Jun protein subunits, their leucine-rich helical sequences entwining into a coiled-coil structure. This study reports on downsizing the proto-oncogene cFos protein (380 residues) to shorter peptides (37-25 residues) modified with helix-inducing constraints to enhance binding to Jun. A crystal structure is reported for a 37-residue Fos-derived peptide (FosW) bound to Jun. This guided iterative downsizing of FosW to shorter peptide sequences that were constrained into stable water-soluble α-helices by connecting amino acid side chains to form cyclic pentapeptide components. Structural integrity in the presence and absence of Jun was assessed by circular dichroism spectroscopy, while the thermodynamics of binding to cFos was measured by isothermal titration calorimetry. A 25-residue constrained peptide, one-third shorter yet 25% more helical than the structurally characterized 37-residue Fos-derived peptide, retained 80% of the binding free energy as a result of preorganization in a Jun-binding helix conformation, with the entropy gain (TΔS = +3.2 kcal/mol) compensating for the enthalpy loss. Attaching a cell-penetrating peptide (TAT48-57) and a nuclear localization signal (SV40) promoted cell uptake, localization to the nucleus, and inhibition of the proliferation of two breast cancer cell lines.

  10. Piperine alleviates osteoclast formation through the p38/c-Fos/NFATc1 signaling axis. (United States)

    Deepak, Vishwa; Kruger, Marlena C; Joubert, Annie; Coetzee, Magdalena


    Increased bone fracture is one of the health risk factors in patients with bone loss related disorders such as osteoporosis and breast cancer metastasis to bone. Over activity of osteoclasts leads to uncoupling of bone remodeling favoring bone loss over bone formation. Receptor activator of nuclear factor-κβ ligand (RANKL) triggers the differentiation pathway leading to multinucleated osteoclast formation. Modulation of RANKL or its downstream signaling pathways involved in osteoclast formation is of significant interest in the development of anti-resorptive agents. In this study, the effects of piperine, an alkaloid present in Piper nigrum L. on osteoclast formation was investigated. Piperine inhibited tartrate-resistant acid phosphatase-positive multinucleated osteoclast formation in murine RAW264.7 macrophages and human CD14+ monocytes induced by RANKL and breast cancer cells. Piperine attenuated the p38-mitogen activated protein kinase pathway activation, while the extracellular-signal-regulated kinase, c-Jun N-terminal kinase, or NF-κβ pathways downstream of RANKL remained unaffected. Concomitantly, expression of c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), the key transcription factors involved in osteoclastogenesis were remarkably inhibited by piperine. Furthermore, piperine disrupted the actin ring structure and bone resorption, a characteristic hallmark of osteoclasts. Collectively, these results suggested that piperine inhibited osteoclast differentiation by suppressing the p38/NFATc1/c-Fos signaling axis.. © 2015 International Union of Biochemistry and Molecular Biology.

  11. c-Fos expression predicts long-term social memory retrieval in mice. (United States)

    Lüscher Dias, Thomaz; Fernandes Golino, Hudson; Moura de Oliveira, Vinícius Elias; Dutra Moraes, Márcio Flávio; Schenatto Pereira, Grace


    The way the rodent brain generally processes socially relevant information is rather well understood. How social information is stored into long-term social memory, however, is still under debate. Here, brain c-Fos expression was measured after adult mice were exposed to familiar or novel juveniles and expression was compared in several memory and socially relevant brain areas. Machine Learning algorithm Random Forest was then used to predict the social interaction category of adult mice based on c-Fos expression in these areas. Interaction with a familiar co-specific altered brain activation in the olfactory bulb, amygdala, hippocampus, lateral septum and medial prefrontal cortex. Remarkably, Random Forest was able to predict interaction with a familiar juvenile with 100% accuracy. Activity in the olfactory bulb, amygdala, hippocampus and the medial prefrontal cortex were crucial to this prediction. From our results, we suggest long-term social memory depends on initial social olfactory processing in the medial amygdala and its output connections synergistically with non-social contextual integration by the hippocampus and medial prefrontal cortex top-down modulation of primary olfactory structures. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Topical dura mater application of CFA induces enhanced expression of c-fos and glutamate in rat trigeminal nucleus caudalis: attenuated by KYNA derivate (SZR72). (United States)

    Lukács, M; Warfvinge, K; Tajti, J; Fülöp, F; Toldi, J; Vécsei, L; Edvinsson, L


    Migraine is a debilitating neurological disorder where trigeminovascular activation plays a key role. We have previously reported that local application of Complete Freund's Adjuvant (CFA) onto the dura mater caused activation in rat trigeminal ganglion (TG) which was abolished by a systemic administration of kynurenic acid (KYNA) derivate (SZR72). Here, we hypothesize that this activation may extend to the trigeminal complex in the brainstem and is attenuated by treatment with SZR72. Activation in the trigeminal nucleus caudalis (TNC) and the trigeminal tract (Sp5) was achieved by application of CFA onto the dural parietal surface. SZR72 was given intraperitoneally (i.p.), one dose prior CFA deposition and repeatedly daily for 7 days. Immunohistochemical studies were performed for mapping glutamate, c-fos, PACAP, substance P, IL-6, IL-1β and TNFα in the TNC/Sp5 and other regions of the brainstem and at the C1-C2 regions of the spinal cord. We found that CFA increased c-fos and glutamate immunoreactivity in TNC and C1-C2 neurons. This effect was mitigated by SZR72. PACAP positive fibers were detected in the fasciculus cuneatus and gracilis. Substance P, TNFα, IL-6 and IL-1β immunopositivity were detected in fibers of Sp5 and neither of these molecules showed any change in immunoreactivity following CFA administration. This is the first study demonstrating that dural application of CFA increases the expression of c-fos and glutamate in TNC neurons. Treatment with the KYNA analogue prevented this expression.

  13. A study of neurotoxic biomarkers, c-fos and GFAP after acute exposure to GSM radiation at 900 MHz in the picrotoxin model of rat brains. (United States)

    Carballo-Quintás, M; Martínez-Silva, I; Cadarso-Suárez, C; Alvarez-Figueiras, M; Ares-Pena, F J; López-Martín, E


    The acute effects of microwave exposure from the Global System for Mobile Communication (GSM) were studied in rats, using 900MHz radiation at an intensity similar to mobile phone emissions. Acute subconvulsive doses of picrotoxin were then administered to the rats and an experimental model of seizure-proneness was created from the data. Seventy-two adult male Sprague-Dawley rats underwent immunochemical testing of relevant anatomical areas to measure induction of the c-fos neuronal marker after 90min and 24h, and of the glial fibrillary acidic protein (GFAP) 72h after acute exposure to a 900MHz electromagnetic field (EMF). The experimental set-up facilitated measurement of absorbed power, from which the average specific absorption rate was calculated using the finite-difference time-domain (FDTD) 2h after exposure to EMF radiation at 1.45W/kg in picrotoxin-treated rats and 1.38W/kg in untreated rats. Ninety minutes after radiation high levels of c-fos expression were recorded in the neocortex and paleocortex along with low hippocampus activation in picrotoxin treated animals. Most brain areas, except the limbic cortical region, showed important increases in neuronal activation 24h after picrotoxin and radiation. Three days after picrotoxin treatment, radiation effects were still apparent in the neocortex, dentate gyrus and CA3, but a significant decrease in activity was noted in the piriform and entorhinal cortex. During this time, glial reactivity increased with every seizure in irradiated, picrotoxin-treated brain regions. Our results reveal that c-fos and glial markers were triggered by the combined stress of non-thermal irradiation and the toxic effect of picrotoxin on cerebral tissues. Copyright © 2011 Elsevier Inc. All rights reserved.

  14. Effects of acute millimeter wave exposure on the expression of substance P and c-fos in rat spinal cord

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    Yan-wen ZHANG


    Full Text Available Objective  To observe the expression changes in substance P (SP and c-fos in rat spinal cord after acute millimeter-wave (MMW exposure, and explore the mechanism of thermal hyperalgesia at the spinal level. Methods  The back skin of SD rats was exposed to 35 GHz MMW (40W/cm2 for 0s (control group, 30s, 1min, or 3min. The corresponding segment of the spinal cord was taken at 0min, 5min, 10min, 1h and 3h after MMW irradiation for total RNA and protein extraction. The expressions of SP and c-fos mRNA were measured by real-time RT-PCR, and the expression of c-fos protein was detected by Western blotting. Results  No significant difference was found between the control group and irradiation groups in SP and c-fos mRNA expression in the corresponding segment of spinal cord after MMW irradiation for 30s. After MMW irradiation for 1min, the SP and c-fos mRNA expressions in the corresponding segment of spinal cord increased significantly at 10min time point, and then decreased to the level of control group. After MMW irradiation for 3min, the SP and c-fos mRNA expression in the corresponding segment of spinal cord increased significantly at 5min, 10min and 1h time points, and decreased to the level of control group at 3h. No significant change was found in c-fos protein expression in the corresponding segment of spinal cord after MMW irradiation for 30s and 1min. After MMW irradiation for 3min, the c-fos protein expression in the corresponding segment of spinal cord increased significantly at 5min and 10min time point, and then decreased to the level of control group. Conclusion  The increase of SP expression in rat skin after MMW irradiation may be related to the increase of SP and c-fos expressions in the corresponding segment of the spinal cord induced by thermal pain stimulation.

  15. HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells

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    Feixin Liang


    Full Text Available To date, the major role of HPV16E6 in cancer has been considered to be its ability to inhibit the p53 tumor-suppressor protein, thereby thwarting p53-mediated cytotoxic responses to cellular stress signals. Here, we show that HPV16E6-dependent c-fos oncogenic protein expression contributes to AP-1 complex formation under oxidative stress in SiHa cells (HPV16-positive squamous cell carcinoma of the cervix. In addition, we examined the role of HPV16E6 in TGF-α-induced c-fos expression and found that the c-fos protein expression induced by TGF-α is HPV16E6 dependent. Thus, our results provide the first evidence that HPV16E6 contributes to AP-1 complex formation after both ligand-dependent and independent EGFR activation, suggesting a new therapeutic approach to the treatment of HPV-associated tumors.

  16. Delta FosB mediates epigenetic desensitization of the c-fos gene after chronic amphetamine exposure. (United States)

    Renthal, William; Carle, Tiffany L; Maze, Ian; Covington, Herbert E; Truong, Hoang-Trang; Alibhai, Imran; Kumar, Arvind; Montgomery, Rusty L; Olson, Eric N; Nestler, Eric J


    The molecular mechanisms underlying the transition from recreational drug use to chronic addiction remain poorly understood. One molecule implicated in this process is DeltaFosB, a transcription factor that accumulates in striatum after repeated drug exposure and mediates sensitized behavioral responses to psychostimulants and other drugs of abuse. The downstream transcriptional mechanisms by which DeltaFosB regulates drug-induced behaviors are incompletely understood. We reported previously the chromatin remodeling mechanisms by which DeltaFosB activates the expression of certain genes; however, the mechanisms underlying DeltaFosB-mediated gene repression remain unknown. Here, we identify c-fos, an immediate early gene rapidly induced in striatum after acute psychostimulant exposure, as a novel downstream target that is repressed chronically by DeltaFosB. We show that accumulation of DeltaFosB in striatum after chronic amphetamine treatment desensitizes c-fos mRNA induction to a subsequent drug dose. DeltaFosB desensitizes c-fos expression by recruiting histone deacetylase 1 (HDAC1) to the c-fos gene promoter, which, in turn, deacetylates surrounding histones and attenuates gene activity. Accordingly, local knock-out of HDAC1 in striatum abolishes amphetamine-induced desensitization of the c-fos gene. In concert, chronic amphetamine increases histone H3 methylation on the c-fos promoter, a chromatin modification also known to repress gene activity, as well as expression levels of the H3 histone methyltransferase, KMT1A (lysine methyltransferase 1A, formerly SUV39H1). This study reveals a novel epigenetic pathway through which DeltaFosB mediates distinct transcriptional programs that may ultimately alter behavioral plasticity to chronic amphetamine exposure.

  17. Hypertonic stress induces c-fos but not c-jun expression in the human embryonal EUE epithelial cell line. (United States)

    Rossi, D; Fuhrman Conti, A M; De Grada, L; Larizza, L


    Recent evidence has indicated a role for the two early response genes c-fos and c-jun in transcriptional regulation of genes acting in osmoregulation. On this basis we investigated their expression in response to hypertonic stress in the human embryonal EUE epithelial cell line. EUE cells have proven to be a useful tool for studying long-term in vitro adaptation to hypertonic stress. After culturing EUE cells in hypertonic medium a marked c-fos induction was observed, both at the mRNA and the protein level. Northern analysis of fos-mRNA showed a peak expression at 4 h, followed by a progressive decline till complete extinction at 8 h. Immunofluorescence analysis of FOS protein evidenced a similar, although slightly delayed kinetics of expression. Conversely, neither c-jun nor c-myc up-regulation could be detected. The treatment of EUE cells with cycloheximide led to superinduction of c-fos expression, (with high levels up to 12 h), and to a c-jun expression that was just detectable. Hypertonic stimulation of the transformed cell lines A549, MCF7 and JR induced both c-fos and c-jun only in JR cells. Hypertonic shock was also effective in inducing c-fos expression in fetal human diploid fibroblasts, although the response was earlier and more transient than in EUE cells. These findings indicate that c-fos is a primary response gene in hypertonic stress-activated cells, although the pattern and kinetics of its induction may differ according to the type of cell.

  18. Differential control of proto-oncogene c-myc and c-fos expression in lymphocytes and fibroblasts. (United States)

    McNerney, R; Darling, D; Johnstone, A


    In lymphocytes stimulated with the mitogen phytohaemagglutinin, an inhibitor of the enzyme ADP-ribosyltransferase (ADPRT) completely blocks the proliferative response and the increase in expression of the proto-oncogene c-myc without affecting c-fos significantly. Conversely, in fibroblasts the serum-induced growth is not affected by the ADPRT inhibitor, and both oncogenes are dramatically super-induced. Hence there are differences between lymphocyte and fibroblast early responses to mitogenic stimulation and also between regulation of c-fos and c-myc gene expression. Images Fig. 2. Fig. 3. PMID:3117047

  19. Daily scheduled high fat meals moderately entrain behavioral anticipatory activity, body temperature, and hypothalamic c-Fos activation.

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    Christian M Gallardo

    Full Text Available When fed in restricted amounts, rodents show robust activity in the hours preceding expected meal delivery. This process, termed food anticipatory activity (FAA, is independent of the light-entrained clock, the suprachiasmatic nucleus, yet beyond this basic observation there is little agreement on the neuronal underpinnings of FAA. One complication in studying FAA using a calorie restriction model is that much of the brain is activated in response to this strong hunger signal. Thus, daily timed access to palatable meals in the presence of continuous access to standard chow has been employed as a model to study FAA in rats. In order to exploit the extensive genetic resources available in the murine system we extended this model to mice, which will anticipate rodent high fat diet but not chocolate or other sweet daily meals (Hsu, Patton, Mistlberger, and Steele; 2010, PLoS ONE e12903. In this study we test additional fatty meals, including peanut butter and cheese, both of which induced modest FAA. Measurement of core body temperature revealed a moderate preprandial increase in temperature in mice fed high fat diet but entrainment due to handling complicated interpretation of these results. Finally, we examined activation patterns of neurons by immunostaining for the immediate early gene c-Fos and observed a modest amount of entrainment of gene expression in the hypothalamus of mice fed a daily fatty palatable meal.

  20. Deep brain stimulation of the mediodorsal thalamic nucleus yields increases in the expression of zif-268 but not c-fos in the frontal cortex. (United States)

    Ewing, Samuel G; Porr, Bernd; Pratt, Judith A


    This study explores the regions activated by deep brain stimulation of the mediodorsal thalamic nucleus through examination of immediate early genes as markers of neuronal activation. Stimulation was delivered unilaterally with constant current 100 μs duration pulses at a frequency of 130 Hz delivered at an amplitude of 200 μA for 3h. Brains were removed, sectioned and radio-labelled for the IEGs zif-268 and c-fos. In anaesthetised rats, deep brain stimulation of mediodorsal thalamic nucleus produced robust increases in the expression of zif-268 but not c-fos localised to regions that are reciprocally connected with the mediodorsal thalamic nucleus, including the prelimbic and orbitofrontal cortices, and the premotor cortex indicating an increase in synaptic activity in these regions. These findings map those brain regions that are persistently, rather than transiently, activated by high frequency electrical stimulation of the mediodorsal thalamic nucleus by a putatively antidromic mechanism which may be relevant to neuropsychiatric disorders such as schizophrenia in which thalamocortical systems are disrupted and in which DBS protocols are being considered. Copyright © 2013 Elsevier B.V. All rights reserved.

  1. Effect of resveratrol on c-fos expression of rat trigeminal spinal nucleus caudalis and C1 dorsal horn neurons following mustard oil-induced acute inflammation. (United States)

    Matsumoto, Yasuhiro; Komatsu, Kyouhei; Shimazu, Yoshihito; Takehana, Shiori; Syouji, Yumiko; Kobayashi, Ayumu; Takeda, Mamoru


    The dietary constituent, resveratrol, was recently identified as a transient receptor potential ankyrin 1 (TRPA1) antagonist, voltage-dependent sodium ion (Na+ ) channel, and cyclooxygenase-2 (COX-2) inhibitor. The aim of the present study was to investigate whether pretreatment with resveratrol attenuates acute inflammation-induced sensitization of nociceptive processing in rat spinal trigeminal nucleus caudalis (SpVc) and upper cervical (C1) dorsal horn neurons, via c-fos immunoreactivity. Mustard oil (MO), a TRPA1 channel agonist, was injected into the whisker pads of rats to induce inflammation. Pretreatment with resveratrol significantly decreased the mean thickness of inflammation-induced edema in whisker pads compared with those of untreated, inflamed rats. Ipsilateral of both the superficial and deep laminae of SpVc and C1 dorsal horn, there were significantly more c-fos-immunoreactive SpVc/C1 neurons in inflamed rats compared with naïve rats, and resveratrol pretreatment significantly decreased that number relative to untreated, inflamed rats. These results suggest that systemic administration of resveratrol attenuates acute inflammation-induced augmented nociceptive processing of trigeminal SpVc and C1 neurons. These findings support resveratrol as a potential therapeutic agent for use in alternative, complementary medicine to attenuate, or even prevent, acute trigeminal inflammatory pain. © 2017 Eur J Oral Sci.

  2. Mediation of suppression of c-fos transcription in rasT24-transformed rat cells by a cis-acting repressor element. (United States)

    Osei-Frimpong, J; Sepulveda, J; Rangdaeng, S; Lebovitz, R M


    Prolonged expression of activated ras mutants resulted in both neoplastic transformation and suppression of serum-induced c-fos expression in Rat1 fibroblasts. Expression of other serum-inducible genes, including c-jun and beta-actin, was not suppressed in ras-transformed Rat1 cells, indicating that these effects are specific for c-fos and that growth-factor signal transduction pathways remain essentially intact. Run-on transcription studies indicated that c-fos transcription was blocked at the level of initiation in these cells. Transient transfection studies using 360 bp from the wild-type c-fos promoter as well as a series of mutated c-fos promoter fragments linked to the chloramphenicol acetyltransferase gene indicated that repression of c-fos was mediated by approximately 49 bp immediately upstream of the dyad symmetry element (DSE). Deletion of this region, referred to as the upstream repressor region (URR), restored serum inducibility to the c-fos promoter in ras-transformed cells. In contrast, suppression of c-fos transcription was not affected by either deletion of 240 bp between the DSE and the TATA element or by base-substitution mutations that inactive the ternary complex factor and fos-AP-1-like binding sites. In addition, in vitro competition studies indicated that ras-transformed cells express one or more repressor factors that interact with as-yet-unidentified elements within the c-fos promoter (possibly the URR) and block serum induction of c-fos. These findings suggest that prolonged expression of activated ras results in the activation of one or more as-yet-unidentified proteins that suppress transcription of the c-fos gene by interacting with the URR.

  3. C/EBPα is Regulated by the RANK Cytoplasmic IVVY535-538 Motif and Stimulates Osteoclastogenesis more Strongly than c-Fos. (United States)

    Jules, Joel; Chen, Wei; Feng, Xu; Li, Yi-Ping


    Binding of RANKL to its receptor RANK on osteoclast (OC) precursors upregulates c-Fos and C/EBPα, two critical OC transcription factors. However, the effects of c-Fos and C/EBPα on osteoclastogenesis have not been compared. Herein, we demonstrate that overexpression of c-Fos or C/EBPα in OC precursors upregulates OC genes and initiates osteoclastogenesis independently of RANKL. However, while C/EBPα upregulated c-Fos, c-Fos failed to upregulate C/EBPα in OC precursors. Consistently, C/EBPα overexpression more strongly promoted OC differentiation than did c-Fos overexpression. RANK has a cytoplasmic IVVY535-538 (IVVY) motif that is essential for osteoclastogenesis, and we found that mutation of the IVVY motif blocked OC differentiation by partly inhibiting expression of C/EBPα but not expression of c-Fos. We therefore hypothesized that C/EBPα overexpression might rescue osteoclastogenesis in cells expressing the mutated IVVY motif. However, overexpression of C/EBPα or c-Fos failed to stimulate osteoclastogenesis in the mutant cells. Notably, the IVVY motif mutation abrogated OC gene expression compared with a vector control, suggesting that the IVVY motif might counteract OC inhibitors during osteoclastogenesis. Consistently, the IVVY motif mutant triggered upregulation of RBP-J protein, a potent OC inhibitor. Mechanistically, C/EBPα or c-Fos overexpression in the mutant cells failed to control the upregulated RBP-J expression, leading to suppression of OC genes. Accordingly, RBP-J silencing in the mutant cells rescued osteoclastogenesis with C/EBPα or c-Fos overexpression, with C/EBPα exhibiting a stronger osteoclastogenic effect. Collectively, our findings indicate that C/EBPα is a stronger inducer of OC differentiation than c-Fos, partly via C/EBPα regulation by the RANK IVVY535-538 motif. Copyright © 2017, The American Society for Biochemistry and Molecular Biology.

  4. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene.

    Directory of Open Access Journals (Sweden)

    Aya Fukuda

    Full Text Available The c-fos gene responds to extracellular stimuli and undergoes robust but transient transcriptional activation. Here we show that heterogeneous nuclear ribonucleoprotein R (hnRNP R facilitates transcription reinitiation of the c-fos promoter in vitro in cooperation with Mediator. Consistently, hnRNP R interacts with the Scaffold components (Mediator, TBP, and TFIIH as well as TFIIB, which recruits RNA polymerase II (Pol II and TFIIF to Scaffold. The cooperative action of hnRNP R and Mediator is diminished by the cyclin-dependent kinase 8 (CDK8 module, which is comprised of CDK8, Cyclin C, MED12 and MED13 of the Mediator subunits. Interestingly, we find that the length of the G-free cassettes, and thereby their transcripts, influences the hnRNP R-mediated facilitation of reinitiation. Indeed, indicative of a possible role of the transcript in facilitating transcription reinitiation, the RNA transcript produced from the G-free cassette interacts with hnRNP R through its RNA recognition motifs (RRMs and arginine-glycine-glycine (RGG domain. Mutational analyses of hnRNP R indicate that facilitation of initiation and reinitiation requires distinct domains of hnRNP R. Knockdown of hnRNP R in mouse cells compromised rapid induction of the c-fos gene but did not affect transcription of constitutive genes. Together, these results suggest an important role for hnRNP R in regulating robust response of the c-fos gene.

  5. Chronic wheel running affects cocaine-induced c-Fos expression in brain reward areas in rats. (United States)

    Zlebnik, Natalie E; Hedges, Valerie L; Carroll, Marilyn E; Meisel, Robert L


    Emerging evidence from human and animal studies suggests that exercise is a highly effective treatment for drug addiction. However, most work has been done in behavioral models, and the effects of exercise on the neurobiological substrates of addiction have not been identified. Specifically, it is unknown whether prior exercise exposure alters neuronal activation of brain reward circuitry in response to drugs of abuse. To investigate this hypothesis, rats were given 21 days of daily access to voluntary wheel running in a locked or unlocked running wheel. Subsequently, they were challenged with a saline or cocaine (15 mg/kg, i.p.) injection and sacrificed for c-Fos immunohistochemistry. The c-Fos transcription factor is a measure of cellular activity and was used to quantify cocaine-induced activation of reward-processing areas of the brain: nucleus accumbens (NAc), caudate putamen (CPu), medial prefrontal cortex (mPFC), and orbitofrontal cortex (OFC). The mean fold change in cocaine-induced c-Fos cell counts relative to saline-induced c-Fos cell counts was significantly higher in exercising compared to control rats in the NAc core, dorsomedial and dorsolateral CPu, the prelimbic area, and the OFC, indicating differential cocaine-specific cellular activation of brain reward circuitry between exercising and control animals. These results suggest neurobiological mechanisms by which voluntary wheel running attenuates cocaine-motivated behaviors and provide support for exercise as a novel treatment for drug addiction. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. Bcl-2, Bax, and c-Fos expression correlates to RPE cell apoptosis induced by UV-light and daunorubicin

    DEFF Research Database (Denmark)

    Liang, Y G; Jorgensen, A G; Kaestel, C G


    PURPOSE. The aim of this study was to determine the role of Bcl-2, Bcl-X L, Bax, and c-Fos in regulation of apoptosis, induced by ultraviolet-light A (UV-A) and daunorubicin (DNR), in retinal pigment epithelium (RPE) cells grown on bovine extracellular matrix (ECM)-coated or uncoated plastic dish...

  7. Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice : spinal cord c-Fos expression and behavior

    NARCIS (Netherlands)

    Eijkelkamp, Niels; Kavelaars, Annemieke; Elsenbruch, Sigrid; Schedlowski, Manfred; Holtmann, Gerald; Heijnen, Cobi J.


    Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior. Am J Physiol Gastrointest Liver Physiol 293: G749-G757, 2007. First published July 26, 2007; doi:10.1152/ajpgi.00114.2007.During acute and chronic inflammation visceral pain

  8. Neuronal expression of c-Fos after epicortical and intracortical electric stimulation of the primary visual cortex. (United States)

    Neyazi, Belal; Schwabe, Kerstin; Alam, Mesbah; Krauss, Joachim K; Nakamura, Makoto


    Electrical stimulation of the primary visual cortex (V1) is an experimental approach for visual prostheses. We here compared the response to intracortical and epicortical stimulation of the primary visual cortex by using c-Fos immunoreactivity as a marker for neuronal activation. The primary visual cortex of male Sprague Dawley rats was unilaterally stimulated for four hours using bipolar electrodes placed either intracortically in layer IV (n=26) or epicortically (n=20). Four different current intensities with a constant pulse width of 200μs and a constant frequency of 10Hz were used, for intracortical stimulation with an intensity of 0μA (sham-stimulation), 10μA, 20μA and 40μA, and for epicortical stimulation 0μA, 400μA, 600μA and 800μA. Subsequently all animals underwent c-Fos immunostaining and c-Fos expression was assessed in layer I-VI of the primary visual cortex within 200μm and 400μm distance to the stimulation site. C-Fos expression was higher after intracortical stimulation compared to epicortical stimulation, even though ten times lower current intensities were applied. Furthermore intracortical stimulation resulted in more focal neuronal activation than epicortical stimulation. C-Fos expression was highest after intracortical stimulation with 20μA compared to all other intensities. Epicortical stimulation showed a linear increase of c-Fos expression with the highest expression at 800μA. Sham stimulation showed similar expression of c-Fos in both hemispheres. The contralateral hemisphere was not affected by intracortical or epicortical stimulation of either intensities. In summary, intracortical stimulation resulted in more focal neuronal activation with less current than epicortical stimulation. This model may be used as a simple but reliable model to evaluate electrodes for microstimulation of the primary visual cortex before testing in more complex settings. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. The α4β2 nicotine acetylcholine receptor agonist ispronicline induces c-Fos expression in selective regions of the rat forebrain

    DEFF Research Database (Denmark)

    Jacobsen, Julie; Hansen, Henrik H; Kiss, Alexander


    was found only within its medial part. In addition, an increased number of c-Fos-immunoreactive cells were observed in the central nucleus of the amygdala, and the dorsolateral part of the bed nucleus of the stria terminalis. The restricted distribution of c-Fos to these areas, all of which are directly...

  10. Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells

    DEFF Research Database (Denmark)

    Petersen, Elisabeth D.; Billestrup, N; Nielsen, Jens Høiriis


    Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH...

  11. A dual-immunocytochemical method to localize c-fos protein in specific neurons based on their content of neuropeptides and connectivity

    DEFF Research Database (Denmark)

    Mikkelsen, J D; Larsen, P J; Sørensen, G G


    Enhanced expression of the immediate early gene c-fos has been used as a marker of cellular activation in many different neuronal pathways. We wished to determine the neurochemical content and the connectivity of neurons, in which expression of c-fos is induced. For this purpose, a dual...

  12. Analysis of nociceptive neurones in the rat superior colliculus using c-fos immunohistochemistry. (United States)

    Telford, S; Wang, S; Redgrave, P


    The superior colliculus (SC) has an established role in the sensory guidance of motor commands required to orient an animal towards novel stimuli. In addition to the representations of visual, auditory, and somatosensory stimuli, the SC also contains a large population of nociceptive neurones. The purpose of the present investigation was to see if nociceptive neurones in the SC can be characterised with c-fos immunohistochemistry as a prelude to establishing anatomical connectivity with specific target regions in the brainstem. To ensure comparability with previous electrophysiological investigations, the present study was conducted in animals anaesthetised with urethane. A series of independent issue relating to basic aspects of experimental protocol were investigated. The principal findings were: (i) Despite minimising the exposure of animals to extraneous stimuli, basal levels of immunostaining were observed. (ii) Urethane anaesthesia induced an increase in Fos-like immunoreactivity (FLI) over the basal condition. (iii) No additional labelling was induced by non-noxious tactile stimulation of the hindpaw. (iv) Unilateral noxious mechanical stimulation elicited a reliable increase in FLI over all control conditions. (v) This increase in FLI was expressed bilaterally and restricted largely to the intermediate white layer. (vi) The induction of FLI was related to noxious stimulation intensity. (vii) No reliable differences in the spatial topography of FLI expression were observed when unilateral noxious mechanical stimulation was administered to the face or hind foot. (vii) A higher level of urethane anaesthesia had a generally suppressive effect on FLI expression. (ix) There were no differences in the distribution of FLI induced by noxious mechanical or noxious chemical stimulation. (x) The increase in FLI induced by noxious pinch was abolished by a naloxone reversible pre-treatment with morphine. These data confirm that c-fos immunohistochemistry can be used to

  13. [Na]i -induced c-Fos expression is not mediated by activation of the 5' -promoter containing known transcriptional elements. (United States)

    Haloui, Mounsif; Taurin, Sebastien; Akimova, Olga A; Guo, Deng-Fu; Tremblay, Johanne; Dulin, Nickolai O; Hamet, Pavel; Orlov, Sergei N


    In vascular smooth muscle cells and several other cell types, inhibition of Na(+)/K(+)-ATPase leads to the expression of early response genes, including c-Fos. We designed this study to examine whether or not a putative Na(+) (i)/K(+) (i)-sensitive element is located within the c-Fos 5'-UTR from - 650 to + 103 containing all known response elements activated by 'classic' stimuli, such as growth factors and Ca(2+) (i)-raising compounds. In HeLa cells, the highest increment of c-Fos mRNA content was noted after 6 h of Na(+)/K(+)-ATPase inhibition with ouabain that was abolished by actinomycin D, an inhibitor of RNA synthesis. c-Fos protein accumulation in ouabain-treated cells correlated with a gain of Na(+) (i) and loss of K(+) (i). Augmented c-Fos expression was also observed under inhibition of Na(+)/K(+)-ATPase in K(+)-free medium and in the presence of the Na(+) ionophore monensin. The effect of ouabain on c-Fos expression was sharply attenuated under dissipation of the transmembrane Na(+) gradient, but was preserved in the presence of Ca(2+) chelators and the extracellular regulated kinase inhibitor PD98059, thus indicating an Na(+) (i)-mediated, Ca(2+) (i)- and extracellular regulated kinase-independent mechanism of gene expression. In contrast to massive c-Fos expression, we failed to detect any effect of ouabain on accumulation of luciferase driven by the c-Fos 5'-UTR. Negative results were also obtained in ouabain-treated vascular smooth muscle cells and C11 Madin-Darby canine kidney cells possessing augmented c-Fos expression. Our results reveal that Na(+) (i)-induced c-Fos expression is not mediated by the 5'-UTR containing transcriptional elements activated by growth factors and other 'classic stimuli'.

  14. Cytosolic malate dehydrogenase regulates RANKL-mediated osteoclastogenesis via AMPK/c-Fos/NFATc1 signaling

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Se Jeong [Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Gu, Dong Ryun [Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Center for Metabolic Function Regulation (CMFR), School of Medicine, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Jin, Su Hyun [Center for Metabolic Function Regulation (CMFR), School of Medicine, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Park, Keun Ha [Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Center for Metabolic Function Regulation (CMFR), School of Medicine, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Lee, Seoung Hoon, E-mail: [Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Center for Metabolic Function Regulation (CMFR), School of Medicine, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of); Wonkwang Institute of Biomaterials and Implant, Wonkwang University, Iksan, Jeonbuk 54538 (Korea, Republic of)


    Cytosolic malate dehydrogenase (malate dehydrogenase 1, MDH1) plays pivotal roles in the malate/aspartate shuttle that might modulate metabolism between the cytosol and mitochondria. In this study, we investigated the role of MDH1 in osteoclast differentiation and formation. MDH1 expression was induced by receptor activator of nuclear factor kappa-B ligand (RANKL) treatment. Knockdown of MDH1 by infection with retrovirus containing MDH1-specific shRNA (shMDH1) reduced mature osteoclast formation and bone resorption activity. Moreover, the expression of marker genes associated with osteoclast differentiation was downregulated by shMDH1 treatment, suggesting a role of MDH1 in osteoclast differentiation. In addition, intracellular ATP production was reduced following the activation of adenosine 5′ monophosphate-activated protein kinase (AMPK), a cellular energy sensor and negative regulator of RANKL-induced osteoclast differentiation, in shMDH1-infected osteoclasts compared to control cells. In addition, the expression of c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), a critical transcription factor of osteoclastogenesis, was decreased with MDH1 knockdown during RANKL-mediated osteoclast differentiation. These findings provide strong evidence that MDH1 plays a critical role in osteoclast differentiation and function via modulation of the intracellular energy status, which might affect AMPK activity and NFATc1 expression.

  15. The action of pulse-modulated GSM radiation increases regional changes in brain activity and c-Fos expression in cortical and subcortical areas in a rat model of picrotoxin-induced seizure proneness. (United States)

    López-Martín, E; Bregains, J; Relova-Quinteiro, J L; Cadarso-Suárez, C; Jorge-Barreiro, F J; Ares-Pena, F J


    The action of the pulse-modulated GSM radiofrequency of mobile phones has been suggested as a physical phenomenon that might have biological effects on the mammalian central nervous system. In the present study, GSM-exposed picrotoxin-pretreated rats showed differences in clinical and EEG signs, and in c-Fos expression in the brain, with respect to picrotoxin-treated rats exposed to an equivalent dose of unmodulated radiation. Neither radiation treatment caused tissue heating, so thermal effects can be ruled out. The most marked effects of GSM radiation on c-Fos expression in picrotoxin-treated rats were observed in limbic structures, olfactory cortex areas and subcortical areas, the dentate gyrus, and the central lateral nucleus of the thalamic intralaminar nucleus group. Nonpicrotoxin-treated animals exposed to unmodulated radiation showed the highest levels of neuronal c-Fos expression in cortical areas. These results suggest a specific effect of the pulse modulation of GSM radiation on brain activity of a picrotoxin-induced seizure-proneness rat model and indicate that this mobile-phone-type radiation might induce regional changes in previous preexcitability conditions of neuronal activation.

  16. Norisoboldine suppresses osteoclast differentiation through preventing the accumulation of TRAF6-TAK1 complexes and activation of MAPKs/NF-κB/c-Fos/NFATc1 Pathways.

    Directory of Open Access Journals (Sweden)

    Zhi-Feng Wei

    Full Text Available Norisoboldine (NOR is the main alkaloid constituent in the dry root of Lindera aggregata (Sims Kosterm. (L. strychnifolia Vill.. As reported previously, orally administered NOR displayed a robust inhibition of joint bone destruction present in both mouse collagen-induced arthritis and rat adjuvant-induced arthritis with lower efficacious doses than that required for ameliorating systemic inflammation. This attracted us to assess the effects of NOR on differentiation and function of osteoclasts, primary effector cells for inflammatory bone destruction, to get insight into its anti-rheumatoid arthritis mechanisms. Both RAW264.7 cells and mouse bone marrow-derived macrophages (BMMs were stimulated with RANKL (100 ng/mL to establish osteoclast differentiation models. ELISA, RT-PCR, gelatin zymography, western blotting, immunoprecipitation and EMSA were used to reveal related signalling pathways. NOR (10 and 30 µM, without significant cytotoxicity, showed significant reduction of the number of osteoclasts and the resorption pit areas, and it targeted osteoclast differentiation at the early stage. In conjunction with the anti-resorption effect of NOR, mRNA levels of cathepsin K and MMP-9 were decreased, and the activity of MMP-9 was attenuated. Furthermore, our mechanistic studies indicated that NOR obviously suppressed the ubiquitination of TRAF6, the accumulation of TRAF6-TAK1 complexes and the activation of ERK and p38 MAPK, and reduced the nuclear translocation of NF-κB-p65 and DNA-binding activity of NF-κB. However, NOR had little effect on expressions of TRAF6 or the phosphorylation and degradation of IκBα. Moreover, NOR markedly inhibited expressions of transcription factor NFATc1, but not c-Fos. Intriguingly, the subsequent nuclear translocations of c-Fos and NFATc1 were substantially down-regulated. Hence, we demonstrated for the first time that preventing the differentiation and function of osteoclasts at the early stage was an

  17. The reducing agent Dithiothreitol (DTT) increases expression of c-myc and c- fos protooncogenes in human cells

    DEFF Research Database (Denmark)

    Skouv, J.; Sørensen, Ilona Kryspin; Frandsen, H.


    The objective of the present study was to assess the possible tumour promoting activity of the food mutagen 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP), by studying its influence on the expression of three genes considered to be of relevance in the tumour promotion step....... However, when cells were treated with DTT alone, the expression of c-fos and c-myc was also transiently induced. We therefore conclude that DTT, and not N-OH-PhIP, induced oncogene expression. Induction of both c-fos and c-mye expression by a reducing agent, DTT, which is frequently used in in vitro...

  18. Bioluminescence imaging of c-fos gene expression accompanying filial imprinting in the newly hatched chick brain. (United States)

    Yamaguchi, Shinji; Iikubo, Eiji; Hirose, Naoki; Kitajima, Takaaki; Katagiri, Sachiko; Kawamori, Ai; Fujii-Taira, Ikuko; Matsushima, Toshiya; Homma, Koichi J


    Bioluminescence imaging is a powerful tool for examining gene expression in living animals. Previously, we reported that exogenous DNA could be successfully delivered into neurons in the newly hatched chick brain using electroporation. Here, we show the in vivo bioluminescence imaging of c-fos promoter activity and its upregulation, which is associated with filial imprinting. The upregulation of c-fos gene expression correlated with both the strength of the chicks' approach activity to the training object and the acquisition of memory. The present technique should be a powerful tool for analyzing the time changes in neural activity of certain brain areas in real-time during memory formation, using brains of living animals.

  19. Caffeic Acid Phenethyl Ester from the Twigs of Cinnamomum cassia Inhibits Malignant Cell Transformation by Inducing c-Fos Degradation. (United States)

    Shin, Seung Ho; Lee, Seoung Rak; Lee, Eunjung; Kim, Ki Hyun; Byun, Sanguine


    The twigs of Cinnamomum cassia, commonly referred to as Cinnamomi Ramulus, are widely used as one of the primary ingredients in Chinese/Korean traditional medicines that have anticancer effects. However, the active constituents responsible for its anticancer effects and their molecular mechanisms still remain to be elucidated. Caffeic acid phenethyl ester (CAPE) and caffeic acid (CA) were isolated for the first time from C. cassia using LC-MS-guided phytochemical isolation methods. CAPE significantly suppressed EGF- and TPA-induced cell transformation of JB6 P+ cells at sub-micromolar concentrations, whereas CA, a structurally similar compound to CAPE, had no such effect. The antiproliferative and chemopreventive activity of CAPE was found to arise through the inhibition of AP-1 transcriptional activity via the promotion of c-Fos degradation. These findings demonstrate that CAPE may contribute to the chemopreventive/chemotherapeutic effects of C. cassia through downregulating c-Fos.

  20. Topical dura mater application of CFA induces enhanced expression of c-fos and glutamate in rat trigeminal nucleus caudalis

    DEFF Research Database (Denmark)

    Lukács, M; Warfvinge, K; Tajti, J


    BACKGROUND: Migraine is a debilitating neurological disorder where trigeminovascular activation plays a key role. We have previously reported that local application of Complete Freund's Adjuvant (CFA) onto the dura mater caused activation in rat trigeminal ganglion (TG) which was abolished......) was achieved by application of CFA onto the dural parietal surface. SZR72 was given intraperitoneally (i.p.), one dose prior CFA deposition and repeatedly daily for 7 days. Immunohistochemical studies were performed for mapping glutamate, c-fos, PACAP, substance P, IL-6, IL-1β and TNFα in the TNC/Sp5 and other...... regions of the brainstem and at the C1-C2 regions of the spinal cord. RESULTS: We found that CFA increased c-fos and glutamate immunoreactivity in TNC and C1-C2 neurons. This effect was mitigated by SZR72. PACAP positive fibers were detected in the fasciculus cuneatus and gracilis. Substance P, TNFα, IL-6...

  1. Heparin inhibits Ca2+/calmodulin-dependent kinase II activation and c-fos induction in mesangial cells.


    Miralem, T; Templeton, D M


    Like vascular smooth-muscle cells, rat mesangial cells (RMCs) display an anti-mitogenic response to heparin. In particular, heparin partially suppresses the ability of quiescent RMCs to enter the cell cycle and induce c-fos expression. When the mitogenic stimulus is serum, phorbol ester or platelet-derived growth factor, this response appears to result from the ability of heparin to suppress activation of the extracellular-signal-regulated kinase family of mitogen-activated protein kinases. H...

  2. The AMPA receptor potentiator LY404187 increases cerebral glucose utilization and c-fos expression in the rat. (United States)

    Fowler, Jill H; Whalley, Katherine; Murray, Tracey; O'neill, Michael J; McCulloch, James


    AMPA receptor potentiators enhance AMPA receptor-mediated glutamatergic neurotransmission and may have therapeutic potential as cognitive enhancers or antidepressants. The anatomical basis for the action of AMPA receptor potentiators is unknown. The aim of this study was to determine the effects of the biarylpropylsulfonamide AMPA receptor potentiator, LY404187 (0.05 to 5 mg/kg subcutaneously), upon cerebral glucose utilization and c-fos expression using 14C-2-deoxglucose autoradiography and c-fos immunocytochemistry. LY404187 (0.5 mg/kg) produced significant elevations in glucose utilization in 28 of the 52 anatomical regions analyzed, which included rostral neocortical areas and the hippocampus, as well the dorsal raphe nucleus, lateral habenula, and locus coeruleus. No significant decreases in glucose utilization were observed in any region after LY404187 administration. The increases in glucose utilization with LY404187 (0.5 mg/kg) were blocked by pretreatment with the AMPA receptor antagonist LY293558 (25 mg/kg), indicating that LY404187 acts through AMPA receptor-mediated mechanisms. LY404187 (0.5 mg/kg) also produced increases in c-fos immunoreactivity in the cortex, locus coeruleus, and the dorsal raphe nucleus. These studies demonstrate neuronal activation in key brain areas that are associated with memory processes and thus provide an anatomical basis for the cognitive enhancing effects of AMPA receptor potentiators.

  3. Orosensory deprivation alters taste-elicited c-Fos expression in the parabrachial nucleus of neonatal rats (United States)

    Haino, Toshiyuki; Hironaka, Shouji; Ooka, Takafumi; Tokita, Kenichi; Kubota, Yu; Boughter, John D.; Inoue, Tomio; Mukai, Yoshiharu


    In the present study we examined the effects of neonatal orosensory deprivation on taste-elicited gustatory activity in the rat parabrachial nucleus (PBN) using the functional anatomical marker c-Fos. Animals in three groups (GG, GO and GM) received gastric cannula implantation surgery on postnatal day 9 (P9). Animals in the fourth group (MR) did not receive any surgery. GG rats were fed by infusion of artificial milk directly into the stomach. GO rats were fed by intraoral infusion of artificial milk. GM and MR rats were reared by their mother with free access to mother’s milk, water and rat chow. Rats from all groups were similar in body weight and length by P21. On P21 rats in all groups were intraorally presented with 0.5 M sucrose solution and the brains were extracted and processed for c-Fos immunohistchemistry. Taste-elicited c-Fos expression in both the gustatory waist area, and the external lateral subnucleus of the PBN in rats in the GG group was significantly more robust than in the other three groups. These findings suggest a substantial alteration in orosensory-evoked neuronal response in this nucleus, due to sensory or motor deprivation during a critical developmental stage. PMID:20302893

  4. c-Fos expression in ouabain-treated vascular smooth muscle cells from rat aorta: evidence for an intracellular-sodium-mediated, calcium-independent mechanism. (United States)

    Taurin, Sebastien; Dulin, Nickolai O; Pchejetski, Dimitri; Grygorczyk, Ryszard; Tremblay, Johanne; Hamet, Pavel; Orlov, Sergei N


    In this study, we examined the effect of Na(+)-K(+) pump inhibition on the expression of early response genes in vascular smooth muscle cells (VSMC) as possible intermediates of the massive RNA synthesis and protection against apoptosis seen in ouabain-treated VSMC in our previous experiments. Incubation of VSMC with ouabain resulted in rapid induction of c-Fos protein expression with an approximately sixfold elevation after 2 h of incubation. c-Jun expression was increased by approximately fourfold after 12 h, whereas expression of activating transcription factor 2, cAMP/Ca(2+) response element binding protein (CREB)-1 and c-Myc was not altered. Markedly augmented c-Fos expression was also observed under Na(+)-K(+) pump inhibition in potassium-depleted medium. Na(+)-K(+) pump inhibition triggered c-Fos expression via elevation of the [Na(+)](i)/[K(+)](i) ratio. This conclusion follows from experiments showing the lack of effect of ouabain on c-Fos expression in high-potassium-low-sodium medium and from the comparison of dose responses of Na(+)-K(+) pump activity, [Na(+)](i) and [K(+)](i) content and c-Fos expression to ouabain. A fourfold increment of c-Fos mRNA was revealed 30 min following addition of ouabain to the incubation medium. At this time point, treatment with ouabain resulted in an approximately fourfold elevation of [Na(+)](i) but did not affect [K(+)](i). Augmented c-Fos expression was also observed under VSMC depolarization in high-potassium medium. Increments in both c-Fos expression and (45)Ca uptake in depolarized VSMC were abolished under inhibition of L-type Ca(2+) channels with 0.1 microM nicardipine. Ouabain did not affect the free [Ca(2+)](i) or the content of exchangeable [Ca(2+)](i). Ouabain-induced c-Fos expression was also insensitive to the presence of nicardipine and [Ca(2+)](o), as well as chelators of [Ca(2+)](o) (EGTA) and [Ca(2+)](i) (BAPTA). The effect of ouabain and serum on c-Fos expression was additive. In contrast to serum

  5. Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction.

    Directory of Open Access Journals (Sweden)

    Takamasa Kudo

    Full Text Available Signaling networks are made up of limited numbers of molecules and yet can code information that controls different cellular states through temporal patterns and a combination of signaling molecules. In this study, we used a data-driven modeling approach, the Laguerre filter with partial least square regression, to describe how temporal and combinatorial patterns of signaling molecules are decoded by their downstream targets. The Laguerre filter is a time series model used to represent a nonlinear system based on Volterra series expansion. Furthermore, with this approach, each component of the Volterra series expansion is expanded by Laguerre basis functions. We combined two approaches, application of a Laguerre filter and partial least squares (PLS regression, and applied the combined approach to analysis of a signal transduction network. We applied the Laguerre filter with PLS regression to identify input and output (IO relationships between MAP kinases and the products of immediate early genes (IEGs. We found that Laguerre filter with PLS regression performs better than Laguerre filter with ordinary regression for the reproduction of a time series of IEGs. Analysis of the nonlinear characteristics extracted using the Laguerre filter revealed a priming effect of ERK and CREB on c-FOS induction. Specifically, we found that the effects of a first pulse of ERK enhance the subsequent effects on c-FOS induction of treatment with a second pulse of ERK, a finding consistent with prior molecular biological knowledge. The variable importance of projections and output loadings in PLS regression predicted the upstream dependency of each IEG. Thus, a Laguerre filter with partial least square regression approach appears to be a powerful method to find the processing mechanism of temporal patterns and combination of signaling molecules by their downstream gene expression.

  6. Differential distribution of activated spinal neurons containing glycine and/or GABA and expressing c-fos in acute and chronic pain models. (United States)

    Hossaini, Mehdi; Duraku, Liron S; Saraç, Ciğdem; Jongen, Joost L M; Holstege, Jan C


    The inhibitory transmitters GABA and glycine play an important role in modulating pain transmission, both in normal and in pathological situations. In the present study we have combined in situ hybridization for identifying spinal neurons that use the transmitter(s) glycine and/or GABA (Gly/GABA neurons) with immunohistochemistry for c-fos, a marker for neuronal activation. This procedure was used with acute pain models induced by the injection of capsaicin or formalin; and chronic pain models using Complete Freund's Adjuvant (CFA, chronic inflammation), and the spared nerve injury (SNI) model (neuropathic pain). In all models Gly/GABA neurons were activated as indicated by their expression of c-fos. The pattern of Gly/GABA neuronal activation was different for every model, both anatomically and quantitatively. However, the averaged percentage of activated neurons that were Gly/GABA in the chronic phase (≥20h survival, 46%) was significantly higher than in the acute phase (≤2h survival, 34%). In addition, the total numbers of activated Gly/GABA neurons were similar in both phases, showing that the activation of non-Gly/GABA (presumed excitatory) neurons in the chronic phase decreased. Finally, morphine application equally decreased the total number of activated neurons and activated Gly/GABA neurons. This showed that morphine did not specifically activate Gly/GABA neurons to achieve nociceptive inhibition. The present study shows an increased activity of Gly/GABA neurons in acute and chronic models. This mechanism, together with mechanisms that antagonize the effects of GABA and glycine at the receptor level, may determine the sensitivity of our pain system during health and disease. Copyright © 2010 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  7. Differential effects of central and peripheral injection of interleukin-1 beta on brain c-fos expression and neuroendocrine functions. (United States)

    Rivest, S; Torres, G; Rivier, C


    Cytokines such as interleukin-1 beta (IL-1 beta) alter the activity of the hypothalamic-pituitary-adrenal (HPA) and hypothalamic-pituitary-gonadal (HPG) axes in the rat. However, the brain sites at which IL-1 beta exerts these effects have not been well identified. The present study sought to identify some of these sites, using c-fos protein expression as an index of cellular activation. We also attempted to determine possible differences between the effects of peripheral and central injection of IL-1 beta on the activation of specific brain areas. Castrated male rats received intravenous (i.v.) or intracerebroventricular (i.c.v.) injections of IL-1 beta through a jugular catheter or a permanent cannula implanted in the right lateral ventricle, respectively. Blood samples were taken before, as well as 30 and 120 min after i.v. or i.c.v. IL-1 beta infusion in order to measure plasma ACTH and LH levels. Immediately thereafter, the rats were anesthetized with pentobarbital, then perfused. Their brains were removed and postfixed for one hour. Thirty-microns frozen sections were cut and approximately every fourth tissue section was processed for c-fos expression by an avidin-biotin-peroxidase method. Both i.v. (1 microgram) and i.c.v. (100 ng) injection of IL-1 beta significantly increased plasma ACTH levels, but only i.c.v. treatment measurably inhibited LH secretion. I.c.v. infusion of the cytokine markedly augmented c-fos expression in the paraventricular nucleus (PVN) and the arcuate nucleus (ARC) of the hypothalamus. A large amount of CRF cells in the PVN contained labelled c-fos protein (as measured by a double labelling technique), which indicates that CRF perikarya in this hypothalamic region are activated by the central administration of IL-1 beta. In contrast, i.v. injection of IL-1 beta did not significantly alter c-fos expression in the PVN or the ARC of the hypothalamus. These results suggest that the increased HPA axis activity which follows the peripheral

  8. The hallucinogen d-lysergic acid diethylamide (d-LSD) induces the immediate-early gene c-Fos in rat forebrain. (United States)

    Frankel, Paul S; Cunningham, Kathryn A


    The hallucinogen d-lysergic acid diethylamide (d-LSD) evokes dramatic somatic and psychological effects. In order to analyze the neural activation induced by this unique psychoactive drug, we tested the hypothesis that expression of the immediate-early gene product c-Fos is induced in specific regions of the rat forebrain by a relatively low, behaviorally active, dose of d-LSD (0.16 mg/kg, i.p.); c-Fos protein expression was assessed at 30 min, and 1, 2 and 4 h following d-LSD injection. A time- and region-dependent expression of c-Fos was observed with a significant increase (PLSD administration. These data demonstrate a unique pattern of c-Fos expression in the rat forebrain following a relatively low dose of d-LSD and suggest that activation of these forebrain regions contributes to the unique behavioral effects of d-LSD. Copyright 2002 Elsevier Science B.V.

  9. Involvement of c-Fos in cell proliferation, migration, and invasion in osteosarcoma cells accompanied by altered expression of Wnt2 and Fzd9.

    Directory of Open Access Journals (Sweden)

    Qiaozhen Wang

    Full Text Available Osteosarcoma (OS is an aggressive bone tumor, and proto-oncogene c-Fos is involved in this lethal disease. However, the role and molecular mechanism of c-Fos in the development and progression of OS remain enigmatic. As one of the Wnt family members, Wnt2 is closely associated with the development of several malignant tumors. In the present study, the expression of c-Fos, Wnt2, and its receptor Fzd9 in human OS tissues, MG63 OS cell line, and human osteoblast hFOB 1.19 cell line was detected by Western blot analysis, immunohistochemical staining, or reverse transcription-polymerase chain reaction. The role of c-Fos in the OS was clarified by treating MG63 cells with small interfering RNA to knockdown c-Fos. Then, cell migration and invasion were assayed by transwell assays and wound healing assay; cell proliferation was assayed by MTS method and 5-ethynyl-2'-deoxyuridine DNA proliferation in vitro detection; cell apoptosis was assayed by flow cytometric method. Co-immunoprecipitation kit was used to confirm the relationship between c-Fos and Wnt2/Fzd9. We found that the expression of c-Fos, Wnt2, and Fzd9 protein was distinctly higher in human OS tissues than that in the adjacent non-cancerous tissues, and their expression in the MG63 OS cell line was markedly increased compared with that in the human osteoblast hFOB 1.19 cell line. Knockdown of c-Fos inhibited the proliferation, migration, and invasion of MG63 cells, and promoted the apoptosis of MG63 cells. Moreover, knockdown of c-Fos inhibited the expression of Wnt2 and Fzd9 mRNA and protein. Our data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9. These findings offer new clues for OS development and progression, and c-Fos may be a potential therapeutic target for OS.

  10. c-fos mRNA is spontaneously induced in the rat brain during the activity period of the circadian cycle. (United States)

    Grassi-Zucconi, G; Menegazzi, M; De Prati, A C; Bassetti, A; Montagnese, P; Mandile, P; Cosi, C; Bentivoglio, M


    The basal expression of the proto-oncogene c-fos was studied by Northern blot analysis in different regions of the rat brain during 24 h. A striking spontaneous oscillation of c-fos mRNA expression was detected in animals kept in basal conditions with a 12 h light/12 h dark cycle. In these animals c-fos mRNA was just detectable during the rest hours (morning through afternoon), and was high during the activity hours (night). The periodicity of this oscillation persisted and became free-running when the animals were exposed for 6 consecutive days to constant light or darkness. It was thus demonstrated that the fluctuation of c-fos expression is circadian and is not created by the light-dark cycle, but the latter exerts a synchronizing effect. The oscillation of c-fos mRNA was modified by manipulations of the rest-activity cycle. In particular, the fluctuation observed in basal conditions was inverted, keeping the animals awake during the rest hours (diurnal) and allowing them to sleep in the activity period (nocturnal). These data indicated a close relationship between the oscillation of c-fos expression and the rest-activity cycle. Finally, electroencephalographic (EEG) monitoring was performed under behavioural control for 3 h before the animals were killed. These experiments confirmed that, irrespective of the time of day, the EEG pattern typical of a state of sleep (including both slow waves and paradoxical sleep) was associated with low or undetectable c-fos levels, whereas the protracted EEG desynchronization corresponding to wakefulness was associated with high c-fos expression.(ABSTRACT TRUNCATED AT 250 WORDS)

  11. Tyrosine kinase activation is an immediate and essential step in hypotonic cell swelling-induced ERK activation and c-fos gene expression in cardiac myocytes.


    Sadoshima, J; Qiu, Z; Morgan, J P; Izumo, S


    Hypotonic stress causes rapid cell swelling and initiates various cellular adaptive processes. However, it is unknown how cells initially sense low osmolarity and convert it into intracellular signals. We investigated the signal transduction mechanism initiated by hypotonic cell swelling in cardiac myocytes using c-fos expression as a nuclear marker. Treatment of myocytes with hypotonic culture media rapidly induced c-fos expression, whereas hypertonic stress had no effect. Transfection of c-...

  12. Analysis of c-Fos induction in response to social interaction in male and female Fisher 344 rats. (United States)

    Perkins, Amy E; Woodruff, Elizabeth R; Chun, Lauren E; Spencer, Robert L; Varlinskaya, Elena; Deak, Terrence


    Sex differences in the expression of social behavior are typically apparent in adolescent and adult rats. While the neurobiology underlying juvenile social play behavior has been well characterized, less is known about discrete brain regions involved in adult responsiveness to a same sex peer. Furthermore, whether adult males and females differ in their responsiveness to a social interaction in terms of neuronal activation indexed via immediate early gene (IEG) expression remains to be determined. Thus, the present study was designed to identify key sites relevant to the processing of sensory stimuli (generally) or social stimuli (specifically) after brief exposure to a same-sex social partner by assessing IEG expression. Four-month-old male and female Fisher (F) 344 rats (N=38; n=5-8/group) were either left undisturbed in their home cage as controls (HCC), exposed to a testing context alone for 30min (CXT), or were placed in the context for 20min and then allowed to socially interact (SI) with a sex-matched conspecific for 10min. Females demonstrated greater levels of social behavior, relative to males. Analysis of c-Fos induction revealed that females exhibited greater c-Fos expression in the prefrontal cortex, regardless of condition. In many brain regions, induction was similar in the CXT and SI groups. However, in the bed nucleus of the stria terminalis (BNST), females exhibited greater c-Fos induction in response to the social interaction relative to their male counterparts, indicating a sex difference in responsivity to social stimuli. Taken together, these data suggest that the BNST is a sexually dimorphic region in terms of activation in response to social stimuli. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Fluid shear stress induction of the transcriptional activator c-fos in human and bovine endothelial cells, HeLa, and Chinese hamster ovary cells. (United States)

    Ranjan, V; Waterbury, R; Xiao, Z; Diamond, S L


    The c-fos protein belongs to a family of transcriptional cofactors that can complex with proteins of the Jun family and activate mRNA transcription from gene promoters containing an activator protein 1 (AP-1) binding element. The shear stress inducibility of the c-fos protein was studied in human and animal cell lines of vastly different origins. Primary human umbilical vein endothelial cells (HUVEC), bovine aortic endothelial cells (BAEC, passage 2-14), HeLa cells, and Chinese hamster ovary (CHO) cells were subjected to steady laminar shear stress using a parallel plate flow apparatus. After 1 h of flow exposure at 25 dyn/cm(2), the c-fos levels in nuclei of shear stress HUVEC, BAEC, HeLa, and CHO were 5.4 +/- 2.0 (n = 3), 2.25 +/- 1.38 (n = 6), 2.14 +/- 0.07 (n = 8), 1.92 +/- 0.58 (n = 2) times higher, respectively, than in matched stationary controls. Flow exposure at 4 dyn/cm(2) caused no enhancement of c-fos levels in any of the cell lines tested, but caused significant reduction in c-fos expression in the HeLa cells. The c-fos induction by shear stress could be blocked by pharmacological agents. For example, the flow induction of the c-fos protein levels was blocked by 50% with the preincubation of HUVEC with a protein kinase C inhibitor, H7 (10 muM) and blocked completely in HeLa cells preincubated with the phospholipase C inhibitor, neomycin (5 mM). The minimum time of shear stress exposure required to induce the c-fos protein expression in HeLa cells was found to be as low as 1 min. By Northern analysis, the c-fos mRNA levels were found to be elevated in BAEC, CHO, and HeLa cells exposed to 25 dyn/cm(2) for 30 min. These studies indicate that c-fos induction is a consistent genetic response in a variety of mammalian cells that may alter cellular phenotype in mechanical environments. (c) 1996 John Wiley & Sons, Inc.

  14. Alcohol induces parallel changes in hippocampal histone H3 phosphorylation and c-fos protein expression in male rats (United States)

    McClain, Justin A.; Nixon, Kimberly


    Background Changes in gene expression associated with alcohol-induced neuroadaptations are controlled in part by post-translational histone modifications. Serine 10 phosphorylation of histone H3 (H3S10ph) has been implicated in drug-induced changes in gene expression; however, ethanol’s effects on H3S10ph have yet to be examined in brain. Therefore, hippocampal H3S10ph was examined after acute alcohol exposure and alcohol dependence. Methods Adult male Sprague-Dawley rats received an acute exposure of ethanol (0–5 g/kg) via gavage. Or, rats were made alcohol dependent by administering 25% w/v ethanol every 8 hours for 4 days following a modified Majchrowicz protocol. In both cases, rats were perfused transcardially and paraformaldehyde-fixed brains were collected and processed for immunohistochemistry for H3S10ph or the immediate early gene, c-fos. Results Acute ethanol exposure dose-dependently altered the number of H3S10ph-positive (+) cells in the hippocampus. Specifically, 1 g/kg ethanol increased the number of H3S10ph+ cells in all neuronal layers, while 2.5 and 5 g/kg ethanol reduced the number of H3S10ph+ cells, an effect that was confined to the granule cell layer (GCL). In ethanol dependent rats, the number of H3S10ph+ cells in the GCL was reduced by 66% during intoxication; however, H3S10ph+ cells were increased in all neuronal layers during peak withdrawal. Subsequent examination of c-fos, a gene known to be regulated by H3S10ph, revealed that ethanol and withdrawal-associated changes in c-fos closely paralleled changes in H3S10ph. Conclusions These results suggest that H3S10ph regulates ethanol-mediated changes in c-fos expression, effects that likely have important implications for ethanol-induced changes in hippocampal neuronal plasticity. PMID:26727528

  15. Apolipoprotein E inhibits osteoclast differentiation via regulation of c-Fos, NFATc1 and NF-κB

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    Kim, Woo-Shin; Kim, Hyung Joon; Lee, Zang Hee [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of); Lee, Youngkyun [Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Kim, Hong-Hee, E-mail: [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of)


    Apolipoprotein E (ApoE) plays a major role in the transport and metabolism of lipid. Other functions of ApoE include modulation of innate and adaptive immune responses. The expression of ApoE in osteoblasts and its relevance with bone formation have also been reported. However, the effect of ApoE on osteoclasts has not yet been examined. Here, we investigated the role of ApoE in osteoclast differentiation using bone marrow-derived macrophages (BMMs) and RAW264.7 cells. We found a down-regulation of ApoE gene expression during osteoclastic differentiation of those cells. Overexpression of ApoE in BMMs and RAW264.7 cells significantly blocked the induction of c-Fos and nuclear factor of activated T cell c1 (NFATc1), transcription factors critical for expression of osteoclast marker genes, by receptor activator of nuclear factor κB ligand (RANKL), the osteoclast differentiation factor. ApoE inhibited osteoclast differentiation, as measured by decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNCs). In addition, ApoE reduced the expression of dendritic cell-specific transmembrane protein (DC-STAMP) and ATPase, H{sup +} transporting, lysosomal 38 kDa, V0 subunit d2 (ATP6v0d2), genes involved in cell–cell fusion during osteoclastogenesis. Knock-down of ApoE using a specific siRNA promoted the RANKL-mediated induction of osteoclast differentiation. While ApoE did not affect the activation of ERK, JNK, and p38 MAPK signaling pathways by RANKL, the phosphorylation of p65 trans-activation domain on serine 536 and transcription activity of NF-κB were reduced by ApoE overexpression. These findings suggest that ApoE plays an inhibitory role in osteoclast differentiation via the suppression of RANKL-dependent activation of NF-κB and induction of c-Fos and NFATc1. - Highlights: ► Apolipoprotein E (ApoE) significantly inhibited osteoclast differentiation and activation of NF-κB. ► ApoE decreased the induction of osteoclast marker

  16. Effect of electrical stimulation of sciatic nerve on synaptic plasticity of spinal dorsal horn and spinal c-fos expression in neonatal, juvenile and adult rats. (United States)

    Wu, Jiang; Hu, Qisheng; Huang, Deying; Chen, Xueling; Chen, Jie


    To explore the response to nociceptive stimuli in spinal cord of immature rat and observe the electrical stimulation of the sciatic nerve on synaptic plasticity of the spinal dorsal horn and spinal c-fos expression in rats of different ages, MK-801 was added to the spinal cord of rats, and the resulting changes in field potential as well as c-fos expression were recorded. LTP in neonatal rats was mainly evoked by A-type nerve fibers, whereas LTP in juvenile and adult rats was mainly evoked by C-type nerve fibers. C-fos expression was significantly increased in the superficial and deep layers of the spinal dorsal horn and in the ventral horn in neonatal rats indicating that the pain signal changed with age. Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.

  17. The transcription factors CREB and c-Fos play key roles in NCAM-mediated neuritogenesis in PC12-E2 cells

    DEFF Research Database (Denmark)

    Jessen, U; Novitskaya, V; Pedersen, N


    The neural cell adhesion molecule (NCAM) stimulates axonal outgrowth by activation of the Ras-mitogen activated protein kinase (MAPK) pathway and by generation of arachidonic acid. We investigated whether the transcription factors, cyclic-AMP response-element binding protein (CREB) and c-Fos play...... roles in this process by estimating NCAM-dependent neurite outgrowth from PC12-E2 cells grown in co-culture with NCAM-negative or NCAM-positive fibroblasts. PC12-E2 cells were transiently transfected with expression plasmids encoding wild-type or dominant negative forms of CREB and c-Fos or an activated......-Fos could compensate for the inactivation of the other, indicating that both factors are important in NCAM-mediated neuritogenesis. Treatment of primary hippocampal neurons with a synthetic NCAM peptide ligand known to stimulate neurite outgrowth induced phosphorylation of CREB and expression of c-fos. We...

  18. Regulation of glucose transport and c-fos and egr-1 expression in cells with mutated or endogenous growth hormone receptors

    DEFF Research Database (Denmark)

    Gong, T W; Meyer, D J; Liao, J


    , a site of association of GHR with the tyrosine kinase JAK2, is crucial for these GH-stimulated responses. As the C-terminal half of the cytoplasmic domain of GHR is required for GH-stimulated calcium flux and for stimulation of spi-2.1 transcription, GHR lacking this sequence (GHR(1-454)) were examined...... is not required for GH-stimulated c-fos transcription, suggesting that increased calcium is not required for GH-stimulated c-fos expression. In CHO cells lacking all but five N-terminal residues of the cytoplasmic domain (GHR(1-294)), GH did not induce c-fos or egr-1 expression or stimulate 2-deoxyglucose uptake...... signaling to the nucleus....

  19. c-Fos immunoreactivity in prefrontal, basal ganglia and limbic areas of the rat brain after central and peripheral administration of ethanol and its metabolite acetaldehyde.

    Directory of Open Access Journals (Sweden)

    Kristen N. Segovia


    Full Text Available Considerable evidence indicates that the metabolite of ethanol (EtOH, acetaldehyde, is biologically active. Acetaldehyde can be formed from EtOH peripherally mainly by alcohol dehydrogenase, and also centrally by catalase. EtOH and acetaldehyde show differences in their behavioral effects depending upon the route of administration. In terms of their effects on motor activity and motivated behaviors, when administered peripherally acetaldehyde tends to be more potent than EtOH but shows very similar potency administered centrally. Since dopamine (DA rich areas have an important role in regulating both motor activity and motivation, the present studies were undertaken to compare the effects of central (intraventricular, ICV and peripheral (intraperitoneal, IP administration of EtOH and acetaldehyde on a cellular marker of brain activity, c-Fos immunoreactivity, in DA innervated areas. Male Sprague-Dawley rats received an IP injection of vehicle, EtOH (0.5 or 2.5 g/kg or acetaldehyde (0.1 or 0.5 g/kg or an ICV injection of vehicle, EtOH or acetaldehyde (2.8 or 14.0 µmoles. IP administration of EtOH minimally induced c-Fos in some regions of the prefrontal cortex and basal ganglia, mainly at the low dose (0.5 g/kg, while IP acetaldehyde induced c-Fos in virtually all the structures studied at both doses. Acetaldehyde administered centrally increased c-Fos in all areas studied, a pattern that was very similar to EtOH. Thus, IP administered acetaldehyde was more efficacious than EtOH at inducing c-Fos expression. However, the general pattern of c-Fos induction promoted by ICV EtOH and acetaldehyde was similar. These results are consistent with the pattern observed in behavioral studies in which both substances produced the same magnitude of effect when injected centrally, and produced differences in potency after peripheral administration.

  20. Comparison of the expression of c-fos, nur77 and egr1 mRNAs in rat hypothalamic magnocellular neurons and their putative afferent projection neurons: cell- and stimulus-specific induction. (United States)

    Luckman, S M


    Hypothalamic magnocellular neurons and their afferent inputs provide a model system in which to study the regulation of inducible transcription factors in the brain in vivo. Osmotic stimulation of rats produced by graded infusions of saline at different tonicities was found to lead to the induction of c-fos, nur77 and egr1 mRNAs in magnocellular neurons, as well as in putative afferent neurons, including those in structures of the forebrain (subfornical organ, median preoptic nucleus and organum vasculosum of the lamina terminalis). The results presented suggest that stronger levels of osmotic stimulation recruit additional afferents from the forebrain and brainstem that can act on magnocellular neurons via alternative receptors. A single systemic injection of the peptide cholecystokinin produced robust induction of c-fos and nur77 mRNAs in afferent neurons of the brainstem nucleus tractus solitarii and in magnocellular neurons. Despite the fact that these two neuronal populations are clearly electrically active, egr1 was not induced by this stimulus, providing examples of cell- and stimulus-specificity of its expression. This study re-emphasizes that the induction of transcription factors is largely dependent on the nature of the afferent input and does not correlate necessarily to the electrical activity of the neuron.

  1. Effect of blonanserin on methamphetamine-induced disruption of latent inhibition and c-Fos expression in rats. (United States)

    Kuramashi, Aki; Abe, Hiroshi; Koganemaru, Go; Matsuo, Hisae; Ikeda, Tetsuya; Ebihara, Kosuke; Funahashi, Hideki; Takeda, Ryuichiro; Nishimori, Toshikazu; Ishida, Yasushi


    To clarify the psychopharmacological profile of blonanserin, a novel antipsychotic, we examined its effect on the methamphetamine-induced disruption of latent inhibition (LI) and the neural activation related to this effect in rats. To evaluate the LI, we used a conditioned emotional response in which a tone (conditioned stimulus) was paired with a mild foot shock (unconditioned stimulus). This paradigm was presented to rats licking water. Methamphetamine-induced (1.0mg/kg, i.p.) disruption of LI was significantly improved by the administration of a higher dose (3.0mg/kg, i.p.) of blonanserin and tended to be improved by 1.0-mg/kg blonanserin and 0.2-mg/kg haloperidol but not by a lower dose (0.3mg/kg) of blonanserin. Immunohistochemical examination showed blonanserin (3.0mg/kg, i.p.) increased c-Fos expression in the shell area but not in the core area of the nucleus accumbens while methamphetamine (3.0mg/kg, i.p.) produced the opposite expression pattern. Blonanserin also increased the number of c-Fos expressions in the central amygdala nucleus but not in the basolateral amygdala nucleus or the prefrontal cortex. Blonanserin ameliorates the methamphetamine-induced disruption of LI, as other antipsychotics do, and a neuronal activation and/or modulation of neurotransmission in the nucleus accumbens is related to the disruption of LI by methamphetamine and to its amelioration by blonanserin. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  2. Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells (United States)

    Aims: To assess virulence of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2. Methods and Results: Aeromonas cells were added to Caco-2 cells at approximately a one to one ratio. After 1, 2 and 3 ...

  3. Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6

    Directory of Open Access Journals (Sweden)

    Saima Mahmood Malhi


    Full Text Available Brain-derived neurotrophic factor (BDNF and c-Fos are shown to promote epileptogenesis and are taken as a marker of neuronal activity. The present study investigated the expression of BDNF and c-Fos in mice brain with pentylenetetrazol- (PTZ- induced generalized seizure and evaluated the effect of novel tryptamine derivative HHL-6 on the expression of these two markers. The subconvulsive dose of PTZ (50 mg/kg was administered on alternate days in the experimental groups until the seizure scores 4-5 developed in the PTZ-control group. At the end of each experiment, animals were sacrificed, brain samples were collected and cryosectioned, and immunohistochemical analysis of BDNF and c-Fos protein was performed. Data obtained from two sections per mouse (n=12 animals/group is presented as means ± S.E.M. The test compound HHL-6 demonstrated a potent anticonvulsant activity in the PTZ-induced seizure in mice. Significant reduction in the BDNF (P<0.003 and c-Fos (P<0.01 protein expression was observed in the HHL-6 treated group. Based on these results we suggest that one of the possible mechanisms of HHL-6 to inhibit epileptogenesis might be due to its controlling effect on the cellular and molecular expression of the factors that contribute to the development of epileptogenic plasticity in the CNS.

  4. Reciprocal Patterns of c-Fos Expression in the Medial Prefrontal Cortex and Amygdala after Extinction and Renewal of Conditioned Fear (United States)

    Knapska, Ewelina; Maren, Stephen


    After extinction of conditioned fear, memory for the conditioning and extinction experiences becomes context dependent. Fear is suppressed in the extinction context, but renews in other contexts. This study characterizes the neural circuitry underlying the context-dependent retrieval of extinguished fear memories using c-Fos immunohistochemistry.…

  5. A role for the immediate early gene product c-fos in imprinting T cells with short-term memory for signal summation.

    Directory of Open Access Journals (Sweden)

    Carolyn E Clark

    Full Text Available T cells often make sequential contacts with multiple DCs in the lymph nodes and are likely to be equipped with mechanisms that allow them to sum up the successive signals received. We found that a period of stimulation as short as two hours could imprint on a T cell a "biochemical memory" of that activation signal that persisted for several hours. This was evidenced by more rapid induction of activation markers and earlier commitment to proliferation upon subsequent stimulation, even when that secondary stimulation occurred hours later. Upregulation of the immediate early gene product c-fos, a component of the AP-1 transcription factor, was maximal by 1-2 hours of stimulation, and protein levels remained elevated for several hours after stimulus withdrawal. Moreover, phosphorylated forms of c-fos that are stable and transcriptionally active persisted for a least a day. Upon brief antigenic stimulation in vivo, we also observed a rapid upregulation of c-fos that could be boosted by subsequent stimulation. Accumulation of phosphorylated c-fos may therefore serve as a biochemical fingerprint of previous suboptimal stimulation, leaving the T cell poised to rapidly resume its activation program upon its next encounter with an antigen-bearing DC.

  6. [Inhibition of histone deacetylases in the chick brain modulates expression of c-Fos and ZENK transcription factors and facilitates establishment of long-term memory]. (United States)

    Toropova, K A; Anokhin, K V; Tiunova, A A


    The aim of the work was to examine the role of histone acetylation in memory consolidation in newborn chicks. We studied the effects of histone deacetylase inhibitor trichostatin A (TSA) on a "weak" memory for passive avoidance and on expression of two transcription factors c-Fos and ZENK known to play a role in neuronal plasticity in the chick brain. Intraventricular administration of trichostatin A prior to training produced a dose-dependent enhancement of memory when tested 24 hours after the training. It also increased neuronal expression of c-Fos and ZENK proteins: the density of ZENK immunopositive cells increased in the hippocampus and intermediate medial mesopallium and the density of c-Fos immunopositive cells increased in intermediate arcopallium and dorsocaudal nidopallium. Weak passive avoidance training did not produce further enhancement of c-Fos and ZENK expression in any of these brain areas. These data demonstrate possibility of facilitating long-term memory in day-old chicks by a histone deacetylases inhibitor, thus supporting the hypothesis on the role of histone acetylation in long-term memory formation. They also suggest that these effects might be mediated through modulation of transcriptional response in brain areas involved in consolidation of this form of memory.

  7. Transcutaneous electrical nerve stimulation on Yongquan acupoint reduces CFA-induced thermal hyperalgesia of rats via down-regulation of ERK2 phosphorylation and c-Fos expression. (United States)

    Yang, Lin; Yang, Lianxue; Gao, Xiulai


    Activation of extracellular signal-regulated kinase-1/2 (ERK1/2) and its involvement in regulating gene expression in spinal dorsal horn, cortical and subcortical neurons by peripheral noxious stimulation contribute to pain hypersensitivity. Transcutaneous electrical nerve stimulation (TENS) is a treatment used in physiotherapy practice to promote analgesia in acute and chronic inflammatory conditions. In this study, a total number of 114 rats were used for three experiments. Effects of complete Freund's adjuvant (CFA)-induced inflammatory pain hypersensitivity and TENS analgesia on ERK1/2 phosphorylation and c-Fos protein expression were examined by using behavioral test, Western blot, and immunostaining methods. We found that CFA injection caused an area of localized swelling, erythema, hypersensitivity to thermal stimuli, the decreased response time of hind paw licking (HPL), as well as upregulation of c-Fos protein expression and ERK2 phosphorylation in the ipsilateral spinal dorsal horn and the contralateral primary somatosensory area of cortex and the amygdala of rats. TENS on Yongquan acupoint for 20 min produced obvious analgesic effects as demonstrated with increased HPL to thermal stimuli of CFA-treated rats. In addition, TENS application suppressed the CFA-induced ERK2 activation and c-Fos protein expression. These results suggest that down-regulation of ERK2 phosphorylation and c-Fos expression were involved in TENS inhibition on CFA-induced thermal hyperalgesia of rats.

  8. Lesions of the fasciculus retroflexus alter footshock-induced cFos expression in the mesopontine rostromedial tegmental area of rats.

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    Paul Leon Brown

    Full Text Available Midbrain dopamine neurons are an essential part of the circuitry underlying motivation and reinforcement. They are activated by rewards or reward-predicting cues and inhibited by reward omission. The lateral habenula (lHb, an epithalamic structure that forms reciprocal connections with midbrain dopamine neurons, shows the opposite response being activated by reward omission or aversive stimuli and inhibited by reward-predicting cues. It has been hypothesized that habenular input to midbrain dopamine neurons is conveyed via a feedforward inhibitory pathway involving the GABAergic mesopontine rostromedial tegmental area. Here, we show that exposing rats to low-intensity footshock (four, 0.5 mA shocks over 20 min induces cFos expression in the rostromedial tegmental area and that this effect is prevented by lesions of the fasciculus retroflexus, the principal output pathway of the habenula. cFos expression is also observed in the medial portion of the lateral habenula, an area that receives dense DA innervation via the fr and the paraventricular nucleus of the thalamus, a stress sensitive area that also receives dopaminergic input. High-intensity footshock (120, 0.8 mA shocks over 40 min also elevates cFos expression in the rostromedial tegmental area, medial and lateral aspects of the lateral habenula and the paraventricular thalamus. In contrast to low-intensity footshock, increases in cFos expression within the rostromedial tegmental area are not altered by fr lesions suggesting a role for non-habenular inputs during exposure to highly aversive stimuli. These data confirm the involvement of the lateral habenula in modulating the activity of rostromedial tegmental area neurons in response to mild aversive stimuli and suggest that dopamine input may contribute to footshock- induced activation of cFos expression in the lateral habenula.

  9. Medial prefrontal cortex neuronal activation and synaptic alterations after stress-induced reinstatement of palatable food seeking: a study using c-fos-GFP transgenic female rats. (United States)

    Cifani, Carlo; Koya, Eisuke; Navarre, Brittany M; Calu, Donna J; Baumann, Michael H; Marchant, Nathan J; Liu, Qing-Rong; Khuc, Thi; Pickel, James; Lupica, Carl R; Shaham, Yavin; Hope, Bruce T


    Relapse to maladaptive eating habits during dieting is often provoked by stress and there is evidence for a role of ovarian hormones in stress responses and feeding. We studied the role of these hormones in stress-induced reinstatement of food seeking and medial prefrontal cortex (mPFC) neuronal activation in c-fos-GFP transgenic female rats, which express GFP in strongly activated neurons. Food-restricted ovariectomized or sham-operated c-fos-GFP rats were trained to lever-press for palatable food pellets. Subsequently, lever-pressing was extinguished and reinstatement of food seeking and mPFC neuronal activation was assessed after injections of the pharmacological stressor yohimbine (0.5-2 mg/kg) or pellet priming (1-4 noncontingent pellets). Estrous cycle effects on reinstatement were also assessed in wild-type rats. Yohimbine- and pellet-priming-induced reinstatement was associated with Fos and GFP induction in mPFC; both reinstatement and neuronal activation were minimally affected by ovarian hormones in both c-fos-GFP and wild-type rats. c-fos-GFP transgenic rats were then used to assess glutamatergic synaptic alterations within activated GFP-positive and nonactivated GFP-negative mPFC neurons following yohimbine-induced reinstatement of food seeking. This reinstatement was associated with reduced AMPA receptor/NMDA receptor current ratios and increased paired-pulse facilitation in activated GFP-positive but not GFP-negative neurons. While ovarian hormones do not appear to play a role in stress-induced relapse of food seeking in our rat model, this reinstatement was associated with unique synaptic alterations in strongly activated mPFC neurons. Our paper introduces the c-fos-GFP transgenic rat as a new tool to study unique synaptic changes in activated neurons during behavior.

  10. Schisandra chinensis and Rhodiola rosea exert an anti-stress effect on the HPA axis and reduce hypothalamic c-Fos expression in rats subjected to repeated stress. (United States)

    Xia, Nan; Li, Jie; Wang, Hongwei; Wang, Jian; Wang, Yangtian


    The aim of the present study was to investigate the effects of Schisandra chinensis (S. chinensis) and Rhodiola rosea (R. rosea) on rats subjected to 5 h of stress, induced by water-floating followed by treadmill exercise. Hypothalamus-pituitary-adrenal (HPA) activity and c-Fos and Fos-related antigen 2 (Fra-2) mRNA expression levels in the hypothalamus of the rats were evaluated. Rats were distributed into four groups: S. chinensis (n=12), R. rosea (n=10), stress control (n=10) and quiet control (n=8). Following a training period of 6 consecutive days, the S. chinensis, R. rosea and stress control groups underwent a 3-h water-floating session in the presence of feline predators immediately followed by 2 h treadmill running to induce psychological and physical stress. Following compound stress induction, the serum levels of corticosterone (CORT), adrenocorticotropic hormone and interleukin-1β and the mRNA expression levels of hypothalamic corticotropin-releasing hormone (CRH), neuropeptide-Y, c-Fos and Fra-2 were evaluated using enzyme-linked immunosorbent assay, radioimmunoassay and quantitative polymerase chain reaction, respectively. The results indicated that S. chinensis and R. rosea markedly decreased the stress-induced elevation of CRH and peripheral CORT levels. The mRNA expression levels of c-Fos and Fra-2 in the hypothalamus were significantly increased after 5 h compound stress, and reduced levels of c-Fos expression were detected in rats treated with R. rosea. Thus, S. chinensis and R. rosea exert an anti-stress effect in rats subjected to stress by balancing the HPA axis, and possibly by reducing the expression of c-Fos in the hypothalamus.

  11. Medial prefrontal cortex neuronal activation and synaptic alterations after stress-induced reinstatement of palatable food seeking: a study using c-fos-GFP transgenic female rats (United States)

    Cifani, Carlo; Koya, Eisuke; Navarre, Brittany M.; Calu, Donna J.; Baumann, Michael H.; Marchant, Nathan J.; Liu, Qing-Rong; Khuc, Thi; Pickel, James; Lupica, Carl R.; Shaham, Yavin; Hope, Bruce T.


    Relapse to maladaptive eating habits during dieting is often provoked by stress and there is evidence for a role of ovarian hormones in stress responses and feeding. We studied the role of these hormones in stress-induced reinstatement of food seeking and medial prefrontal cortex (mPFC) neuronal activation in c-fos-GFP transgenic female rats, which express green fluorescent protein (GFP) in strongly activated neurons. Food-restricted ovariectomized or sham-operated c-fos-GFP rats were trained to lever-press for palatable food pellets. Subsequently, lever-pressing was extinguished and reinstatement of food seeking and mPFC neuronal activation was assessed after injections of the pharmacological stressor yohimbine (0.5–2 mg/kg) or pellet priming (1–4 non-contingent pellets). Estrous cycle effects on reinstatement were also assessed in wild-type rats. Yohimbine- and pellet-priming-induced reinstatement was associated with Fos and GFP induction in mPFC; both reinstatement and neuronal activation were minimally affected by ovarian hormones in both c-fos-GFP and wild-type rats. c-fos-GFP transgenic rats were then used to assess glutamatergic synaptic alterations within activated GFP-positive and non-activated GFP-negative mPFC neurons following yohimbine-induced reinstatement of food seeking. This reinstatement was associated with reduced AMPAR/NMDAR current ratios and increased paired-pulse facilitation in activated GFP-positive but not GFP-negative neurons. Together, while ovarian hormones do not appear to play a role in stress-induced relapse of food seeking in our rat model, this reinstatement was associated with unique synaptic alterations in strongly activated mPFC neurons. Our paper introduces the c-fos-GFP transgenic rat as a new tool to study unique synaptic changes in activated neurons during behavior. PMID:22723688

  12. Cytotoxicity and Expression of c-fos, HSP70, and GADD45/153 Proteins in Human Liver Carcinoma (HepG2 Cells Exposed to Dinitrotoluenes

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    Paul B. Tchounwou


    Full Text Available Dinitrotoluenes (DNTs are byproducts of the explosive trinitrotoluene (TNT, and exist as a mixture of 2 to 6 isomers, with 2,4-DNT and 2,6-DNT being the most significant. The main route of human exposure at ammunition facilities is inhalation. The primary targets of DNTs toxicity are the hematopoietic system, cardiovascular system, nervous system and reproductive system. In factory workers, exposure to DNTs has been linked to many adverse health effects, including: cyanosis, vertigo, headache, metallic taste, dyspnea, weakness and lassitude, loss of appetite, nausea, and vomiting. Other symptoms including pain or parasthesia in extremities, abdominal discomfort, tremors, paralysis, chest pain, and unconsciousness have been documented. An association between DNTs exposure and increased risk of hepatocellular carcinomas and subcutaneous tumors in rats, as well as renal tumors in mice, has been established. This research was therefore designed targeting the liver to assess the cellular and molecular responses of human liver carcinoma cells following exposure to 2,4-DNT and 2,6-DNT. Cytotoxicity was evaluated using the MTT assay. Upon 48 hrs of exposure, LC50 values of 245 + 14.72μg/mL, and 300 + 5.92μg/mL were recorded for 2,6-DNT and 2,4-DNT respectively, indicating that both DNTs are moderately toxic, and 2,6-DNT is slightly more toxic to HepG2 cells than 2,4-DNT. A dose response relationship was recorded with respect to the cytotoxicity of both DNTs. Western blot analysis resulted in a significant expression (p<0.05 of the 70-kDa heat shock protein in 2,6-DNT-treated cells compared to the control cells and at the 200 μg/mL dose for 2,4-DNT. A statistically significant expression in c-fos was also observed at the 200 and 250 μg/mL treatment level for 2,4- and 2,6-DNT, respectively. However, no statistically significant expression of this protooncogene-related protein was observed at the doses of 0, 100, or 300

  13. Light-induced c-Fos expression in the SCN and behavioural phase shifts of Djungarian hamsters with a delayed activity onset. (United States)

    Schöttner, Konrad; Vuillez, Patrick; Challet, Etienne; Pévet, Paul; Weinert, Dietmar


    C-Fos expression in the suprachiasmatic nucleus (SCN) and phase shifts of the activity rhythm following photic stimulation were investigated in Djungarian hamsters (Phodopus sungorus) of two different circadian phenotypes. Wild-type (WT) hamsters display robust daily patterns of locomotor activity according to the light/dark conditions. Hamsters of the DAO (delayed activity onset) phenotype, however, progressively delay the activity onset, whereas activity offset remains coupled to "light-on". Although the exact reason for the delayed activity onset is not yet clarified, it is connected with a disturbed interaction between the light/dark cycle and the circadian clock. The aim was to test the link between photoreception and the behavioral output of the circadian system in hamsters of both phenotypes, to get further insight in the underlying mechanism of the DAO phenomenon. Animals were exposed to short light pulses at different times during the dark period to analyze phase shifts of the activity rhythm and expression of Fos protein in the SCN. The results indicate that the photosensitive phase in DAO hamsters is shifted like the activity onset. Also, phase shifts were significantly smaller in DAO hamsters. At the same time, levels of Fos expression did not differ between phenotypes regarding the circadian phase. The results provide evidence that the shifted photosensitivity of the circadian system in DAO hamsters does not differ from that of WT animals, and lead us to conclude that processes within the SCN that enable light information to reset the circadian pacemaker might offer an explanation for the DAO phenomenon.

  14. Patterns of violent aggression-induced brain c-fos expression in male mice selected for aggressiveness. (United States)

    Haller, J; Tóth, M; Halasz, J; De Boer, S F


    Mice selected for aggressiveness (long and short attack latency mice; LALs and SALs, respectively) constitute a useful tool in studying the neural background of aggressive behavior, especially so as the SAL strain shows violent forms of aggressiveness that appear abnormal in many respects. By using c-Fos staining as a marker of neuronal activation, we show here that agonistic encounters result in different activation patterns in LAL and SAL mice. In LALs, agonistic encounters activated the lateral septum, bed nucleus of stria terminalis, medial amygdala, paraventricular nucleus of the hypothalamus, anterior hypothalamic nucleus and tuber cinereum area (both being analogous with the rat hypothalamic attack area), dorsolateral periaqueductal gray, and locus coeruleus. This pattern is similar with that seen in the territorial aggression of male mice, rats and hamsters, and non-lactating female mice. SALs showed strong fight-induced activations in the central amygdala and lateral/ventrolateral periaqueductal gray. In this strain, no activation was seen in the lateral septum and the dorsolateral periaqueductal gray. This pattern is similar with that seen in other models of violent aggression, e.g., in attacks induced by hypothalamic stimulation in rats, quiet biting in cats, lactating female mice, and hypoarousal-driven abnormal aggression in rats. We suggest here that the excessive activation of the central amygdala and lateral/ventrolateral periaqueductal gray--accompanied by a smaller activation of the septum and dorsolateral periaqueductal gray--underlay the expression of violent attacks under various circumstances.

  15. Functional magnetic resonance imaging and c-Fos mapping in rats following a glucoprivic dose of 2-deoxy-D-glucose. (United States)

    Dodd, Garron T; Williams, Steve R; Luckman, Simon M


    The glucose analogue, 2-deoxy-D-glucose (2-DG) is an inhibitor of glycolysis and, when administered systemically or centrally, induces glucoprivation leading to counter-regulatory responses, including increased feeding behaviour. Investigations into how the brain responds to glucoprivation could have important therapeutic potential, as disruptions or defects in the defence of the brain's 'glucostatic' circuitry may be partly responsible for pathological conditions resulting from diabetes and obesity. To define the 'glucostat' brain circuitry further we have combined blood-oxygen-level-dependent pharmacological-challenge magnetic resonance imaging (phMRI) with whole-brain c-Fos functional activity mapping to characterise brain regions responsive to an orexigenic dose of 2-DG [200 mg/kg; subcutaneous (s.c.)]. For phMRI, rats were imaged using a T(2)*-weighted gradient echo in a 7T magnet for 60 min under alpha-chloralose anaesthesia, whereas animals for immunohistochemistry were unanaesthetised and freely behaving. These complementary methods demonstrated functional brain activity in a number of previously characterised glucose-sensing brain regions such as those in the hypothalamus and brainstem following administration of 2-DG compared with vehicle. As the study mapped whole-brain functional responses, it also identified the orbitofrontal cortex and striatum (nucleus accumbens and ventral pallidum) as novel 2-DG-responsive brain regions. These regions make up a corticostriatal connection with the hypothalamus, by which aspects of motivation, salience and reward can impinge on the hypothalamic control of feeding behaviour. This study, therefore, provides further evidence for a common integrated circuit involved in the induction of feeding behaviour, and illustrates the valuable potential of phMRI in investigating central pharmacological actions.

  16. Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita

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    Kesmati Mahnaz


    Full Text Available Background: There are some evidences that indicate there are sexual differences in drug abuse and response to synthetic and herbal drugs. It has been shown that the expression of C-FOS increases in many areas of brain during morphine withdrawal. Concerning the sedative effect of Matricaria recutita extract, the aim of this study was to compare expression of C-FOS transcription factor during morphine withdrawal with and without acute administration of Matricaria recutita on male and female adult mice.Materials and Methods: This study was done at Shahid Chamran University of Ahvaz in 2007 on NMRI mice. Male and female mice were assigned into 8 groups (morphine + saline; morphine + naloxone; morphine + Matricaria recutita + naloxone; and morphine + saline + naloxone. To develop morphine dependency, increasing doses of morphine (20, 40, 80 mg/kg injected subcutaneously for 4 days. Mice received a final morphine injection (40 mg/kg 3hours prior to naloxone (5 mg/kg on the day of testing (day 4. Matricaria recutita extract whit a dose of 30 mg/kg was administered intraperitoneally 5 minutes before naloxone injection. In cellular study, 90minute after naloxone injection, mice were decapitated and their brains were separated, then mRNA was extracted from brain tissue. Using DIG-labeled DNA probe of C-FOS, beta-actin and dot blot technique, expression of C-FOS was analyzed by Zero Dscan software. Statistical evaluation of data was performed using student t-test and ANOVA with one factor followed by Duncan test in SPSS software. P values less than 0.05 were considered significant. Results: The rate of expression of C-FOS increased in male mice but decreased significantly in female mice after naloxone-precipitated abstinence P<0.01(. Matricaria recutita attenuated the rate of expression of C-FOS in male mice but it showed synergistic effect on it in female mice P<0.05(.Conclusion: It seems that the cellular processes involving morphine dependency and

  17. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex

    DEFF Research Database (Denmark)

    Hale, M.W.; Hay-Schmidt, A.; Mikkelsen, J.D.


    Serotonergic systems in the dorsal raphe nucleus are thought to play an important role in the regulation of anxiety states. To investigate responses of neurons in the dorsal raphe nucleus to a mild anxiety-related stimulus, we exposed rats to an open-field, under low-light or high-light conditions...... of neurons in the midbrain raphe complex that projects to forebrain circuits regulating anxiety states, we used cholera toxin B subunit (CTb) as a retrograde tracer to identify neurons projecting to the basolateral amygdaloid complex (BL) in combination with c-Fos immunostaining to identify cells...... that activated neurons were serotonergic, non-serotonergic, or both. These data are consistent with the hypothesis that exposure to anxiogenic stimuli activates a subset of neurons in the midbrain raphe complex projecting to amygdala anxiety circuits Udgivelsesdato: 2008/12/10...


    DEFF Research Database (Denmark)

    Christensen, S. L. T.; Petersen, S.; Sorensen, D. B.


    in rats. Also, we tested the response to sumatriptan in order to evaluate the predictive properties of the model. Methods: The effect of cilostazol (125 mg/kg p.o.) was evaluated on a range of spontaneous behavioural parameters, light sensitivity and mechanical sensitivity thresholds. To assess headache...... specificity we evaluated the c-fos expression in the trigeminal nucleus caudalis. All experiments were done in female Sprague Dawley rats and the oestrous cycle was included in the analyses. Results: We found that cilostazol increased the light sensitivity and grooming behaviour of the rats and decreased...... their head twitching. These manifestations were not inhibited by sumatriptan. Cilostazol also induced c-fos expression in the trigeminal nucleus caudalis. Locomotion, rearing, eating and drinking activity as well as the amount of wet dog shakes and mechanical sensitivity thresholds were unaltered. Discussion...

  19. Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.

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    Ryan D Walters

    Full Text Available The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.

  20. C-fos induction in forebrain areas of two different visual pathways during consolidation of sexual imprinting in the zebra finch (Taeniopygia guttata). (United States)

    Sadananda, Monika; Bischof, Hans-Joachim


    Two forebrain areas in the hyperpallium apicale and in the lateral nidopallium of isolated male zebra finches are highly active (2-deoxyglucose technique) on exposure to females for the first time, that is first courtship. These areas also demonstrate enhanced neuronal plasticity when screened with c-fos immunocytochemistry. Both are areas involved in the processing of visual information conveyed by the two major visual pathways in birds, strengthening our hypothesis that courtship in the zebra finch is a visually guided behaviour. First courtship and chased birds show enhanced c-fos induction in the hyperpallial area, which could represent neuronal activity reflecting changes in the immediate environment. The enhanced expression of fos in first courtship birds in lateral nidopallial neurons indicates imminent long-lasting changes at the synaptic level that form the substrate for imprinting, a stable form of learning in birds.

  1. Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages. (United States)

    Wang, Dayong; Noda, Yukihiro; Zhou, Yuan; Nitta, Atsumi; Nabeshima, Toshitaka; Yu, Qinghai


    The purpose of this research is to investigate the effects of sodium houttuyfonate on the phosphorylation of CaMK II, CREB and ERK 1/2, and the expression of c-Fos. Macrophages were cultured in vitro with or without sodium houttuyfonate in the culture medium. After cell culture, macrophages were lysed and the lysate of the macrophages was collected for analysis. Western-blotting method was adopted to investigate the phosphorylation or the expression of these signal elements. It was found in this research that the phosphorylation levels of CaMK II and CREB and the expression of c-Fos protein in macrophages were increased by sodium houttuyfonate treatment; however, the phosphorylation level of ERK 1/2 was not affected by the treatment.

  2. Melanocortin 4 receptor activates ERK-cFos pathway to increase brain-derived neurotrophic factor expression in rat astrocytes and hypothalamus. (United States)

    Ramírez, D; Saba, J; Carniglia, L; Durand, D; Lasaga, M; Caruso, C


    Melanocortins are neuropeptides with well recognized anti-inflammatory and anti-apoptotic effects in the brain. Of the five melanocortin receptors (MCR), MC4R is abundantly expressed in the brain and is the only MCR present in astrocytes. We have previously shown that MC4R activation by the α-melanocyte stimulating hormone (α-MSH) analog, NDP-MSH, increased brain-derived neurotrophic factor (BDNF) expression through the classic cAMP-Protein kinase A-cAMP responsive element binding protein pathway in rat astrocytes. Now, we examined the participation of the mitogen activated protein kinases pathway in MC4R signaling. Rat cultured astrocytes treated with NDP-MSH 1 µM for 1 h showed increased BDNF expression. Inhibition of extracellular signal-regulated kinase (ERK) and ribosomal p90 S6 kinase (RSK), an ERK substrate, but not of p38 or JNK, prevented the increase in BDNF expression induced by NDP-MSH. Activation of MC4R increased cFos expression, a target of both ERK and RSK. ERK activation by MC4R involves cAMP, phosphoinositide-3 kinase (PI3K) and the non receptor tyrosine kinase, Src. Both PI3K and Src inhibition abolished NDP-MSH-induced BDNF expression. Moreover, we found that intraperitoneal injection of α-MSH induces BDNF and MC4R expression and activates ERK and cFos in male rat hypothalamus. Our results show for the first time that MC4R-induced BDNF expression in astrocytes involves ERK-RSK-cFos pathway which is dependent on PI3K and Src, and that melanocortins induce BDNF expression and ERK-cFos activation in rat hypothalamus. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  3. Age-specific effects of 6-hydroxydopamine lesions of the rat medial prefrontal cortex on stress-induced c-fos expression in subcortical areas. (United States)

    Sui, Zhi Yan; Li, Chun-Rong; Huang, Guang-Biao; Kwon, Young-Bae; Kim, Kee-Won; Lee, Keon-Hak; Chung, Young-Chul


    As adolescence is a critical period when dopaminergic neuronal maturation peaks, we hypothesized that 6-hydroxydopamine (OHDA) lesions of the medial prefrontal cortex (mPFC) in adolescent rats would have more negative effects than lesions in adult rats. Therefore, we investigated the effects of 6-OHDA lesions of the mPFC in adolescent and adult rats on stress-induced c-fos expression in the brain. Adolescent and adult Sprague-Dawley rats, aged 4 and 7 weeks on arrival, respectively, were studied. 6-OHDA (8.0 microg) for the lesion groups and ascorbic acid for the sham groups were injected bilaterally into the mPFC. All animals were pretreated with desipramine 30 min before being anesthetized. The control group did not undergo any surgery-related procedure except the desipramine injection. After recovery for 1 week, the rats were subjected to restraint stress for 1 h. Immediately after the stress, the rats were killed and c-fos immunohistochemistry was examined. The c-fos expression in the nucleus accumbens core (AcbC), nucleus accumbens shell (AcbSh), CA1, CA3, dentate gyrus (DG), central amygdaloid (Ce), basolateral amygdaloid (BL), and temporal cortex (Tc) was compared. Adolescent rats with 6-OHDA lesions subjected to restraint stress had greater c-fos expression in the AcbC, AcbSh, DG, Ce, BL, and Tc, compared to the sham and control groups, whereas these differences were not observed among the adult groups. These results suggest that a hypodopaminergic state in the mPFC of adolescent rats, but not adult rats, is related to increased sensitivity to stress, suggesting that damage to or maldevelopment of dopaminergic neurons during adolescence has an age-specific effect. Further research is warranted to investigate the mechanism of the age-specific effect of 6-OHDA lesions of the mPFC.

  4. Rapamycin inhibits ox-LDL-induced inflammation in human endothelial cells in vitro by inhibiting the mTORC2/PKC/c-Fos pathway. (United States)

    Sun, Juan-Juan; Yin, Xiao-Wei; Liu, Hui-Hui; Du, Wen-Xiu; Shi, Lu-Yao; Huang, Ya-Bo; Wang, Fen; Liu, Chun-Feng; Cao, Yong-Jun; Zhang, Yan-Lin


    Rapamycin and its derivative possess anti-atherosclerosis activity, but its effects on adhesion molecule expression and macrophage adhesion to endothelial cells during atherosclerosis remain unclear. In this study we explored the effects of rapamycin on ox-LDL-induced adhesion molecule expression and macrophage adhesion to endothelial cells in vitro and the underlying mechanisms. Ox-LDL (6-48 μg/mL) dose-dependently increased the protein levels of two adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and E-selectin, in human umbilical vein endothelial cells (HUVECs), whereas pretreatment with rapamycin (1-10 μmol/L) dose-dependently inhibited ox-LDL-induced increase in the adhesion molecule expression and macrophage adhesion to endothelial cells. Knockdown of mTOR or rictor, rather than raptor, mimicked the effects of rapamycin. Ox-LDL (100 μg/mL) time-dependently increased PKC phosphorylation in HUVECs, which was abolished by rapamycin or rictor siRNA. Pretreatment with PKC inhibitor staurospo¬rine significantly reduced ox-LDL-stimulated adhesion molecule expression and macrophage adhesion to endothelial cells, whereas pretreatment with PKC activator PMA/TPA attenuated the inhibitory effect of rapamycin on adhesion molecule expression. Ox-LDL (100 μg/mL) time-dependently increased c-Fos levels in HUVECs, and pretreatment with rapamycin or rictor siRNA significantly decreased expression of c-Fos. Knockdown of c-Fos antagonized ox-LDL-induced adhesion molecule expression and macrophage adhesion to endothelial cells. Our results demonstrate that rapamycin reduces ox-LDL-stimulated adhesion molecule expression and macrophage adhesion to endothelial cells by inhibiting mTORC2, but not mTORC1, and mTORC2 acts through the PKC/c-Fos signaling pathway.

  5. Effect of teicoplanin on the expression of c-myc and c-fos proto-oncogenes in MCF-7 breast cancer cell line

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    Saeideh Ashouri


    Conclusion: it could be concluded that although teicoplanin is considered as an enhancing cell growth and proliferation, but probably its effect is not through MAP kinase signaling pathway or perhaps even has inhibitory effect on the expression of some genes such as c-myc and c-fos in this pathway. Hence, the mechanism of action of teicoplanin for increasing cell propagation, through cell signaling pathways or chromosomal abnormalities, remains unclear, and further studies should be conducted.

  6. Acute effects of nicotine on restraint stress-induced anxiety-like behavior, c-Fos expression, and corticosterone release in mice. (United States)

    Hsu, Hui-Ru; Chen, Tsung-Yen; Chan, Ming-Huan; Chen, Hwei-Hsien


    Clinical evidence suggests that nicotine reduces anxiety in stressful situations. In the present study, we investigated the effect of nicotine on restraint-enhanced anxiety-like behavior, c-Fos expression, an index of neuronal activation in the brain, and plasma corticosterone. Two-hour restraint stress-enhanced anxiety-like behavior in the elevated plus-maze (EPM) and nicotine hydrogen tartrate (0.25 mg/kg, i.p.) attenuated the stress-induced changes. Pretreatment with the centrally acting nicotinic antagonist, mecamylamine (2 mg/kg), blocked nicotine's effects. In addition, restraint led to significant increases of c-Fos expression in several brain regions related to stress or anxiety including paraventricular hypothalamic nucleus (PVN), lateral hypothalamic area (LH), central amygdaloid nucleus (CeA), medial amygdaloid nucleus (MeA) and cingulate and retrosplenial cortices (Cg/RS), paraventricular thalamic nucleus (PVT), and basolateral amygdaloid nucleus (BLA). Nicotine attenuated the restraint-induced expression of c-Fos in the PVN, LH, CeA, MeA, and Cg/RS, while leaving the BLA and PVT unaffected. In contrast, nicotine did not reverse the increased levels of plasma corticosterone induced by restraint. These findings suggest that nicotine may modify the stress-induced behavioral changes via regulating the neuronal activation in selected brain regions rather than affecting hypothalamo-pituitary-adrenocortical axis hormone responses.

  7. IP{sub 3}-dependent intracellular Ca{sup 2+} release is required for cAMP-induced c-fos expression in hippocampal neurons

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wenting; Tingare, Asmita; Ng, David Chi-Heng [Department of Pharmacology, University of Cambridge (United Kingdom); Johnson, Hong W.; Schell, Michael J. [Department of Pharmacology, Uniformed Services University, Bethesda (United States); Lord, Rebecca L. [Department of Biology, University of York (United Kingdom); Chawla, Sangeeta, E-mail: [Department of Pharmacology, University of Cambridge (United Kingdom); Department of Biology, University of York (United Kingdom)


    Highlights: Black-Right-Pointing-Pointer cAMP-induced c-fos expression in hippocampal neurons requires a submembraneous Ca{sup 2+} pool. Black-Right-Pointing-Pointer The submembraneous Ca{sup 2+} pool derives from intracellular ER stores. Black-Right-Pointing-Pointer Expression of IP{sub 3}-metabolizing enzymes inhibits cAMP-induced c-fos expression. Black-Right-Pointing-Pointer SRE-mediated and CRE-mediated gene expression is sensitive to IP{sub 3}-metabolizing enzymes. Black-Right-Pointing-Pointer Intracellular Ca{sup 2+} release is required for cAMP-induced nuclear translocation of TORC1. -- Abstract: Ca{sup 2+} and cAMP are widely used in concert by neurons to relay signals from the synapse to the nucleus, where synaptic activity modulates gene expression required for synaptic plasticity. Neurons utilize different transcriptional regulators to integrate information encoded in the spatiotemporal dynamics and magnitude of Ca{sup 2+} and cAMP signals, including some that are Ca{sup 2+}-responsive, some that are cAMP-responsive and some that detect coincident Ca{sup 2+} and cAMP signals. Because Ca{sup 2+} and cAMP can influence each other's amplitude and spatiotemporal characteristics, we investigated how cAMP acts to regulate gene expression when increases in intracellular Ca{sup 2+} are buffered. We show here that cAMP-mobilizing stimuli are unable to induce expression of the immediate early gene c-fos in hippocampal neurons in the presence of the intracellular Ca{sup 2+} buffer BAPTA-AM. Expression of enzymes that attenuate intracellular IP{sub 3} levels also inhibited cAMP-dependent c-fos induction. Synaptic activity induces c-fos transcription through two cis regulatory DNA elements - the CRE and the SRE. We show here that in response to cAMP both CRE-mediated and SRE-mediated induction of a luciferase reporter gene is attenuated by IP{sub 3} metabolizing enzymes. Furthermore, cAMP-induced nuclear translocation of the CREB coactivator TORC1 was inhibited

  8. cFos mediates cAMP-dependent generation of ROS and rescue of maturation program in retinoid-resistant acute promyelocytic leukemia cell line NB4-LR1

    National Research Council Canada - National Science Library

    Carrier, Jean-Luc; Javadi, Pasha; Bourrier, Emilie; Camus, Céline; Ségal-Bendirdjian, Evelyne; Karniguian, Aïda


    .... However, the underlying mechanisms have not been elucidated yet. Here, we identify cFos as a critical cAMP effector, able to regulate the re-expression and splicing of epigenetically silenced genes associated with maturation (CD44...

  9. Regulation of the JNK3 signaling pathway during islet isolation: JNK3 and c-fos as new markers of islet quality for transplantation.

    Directory of Open Access Journals (Sweden)

    Saida Abdelli

    Full Text Available Stress conditions generated throughout pancreatic islet processing initiate the activation of pro-inflammatory pathways and beta-cell destruction. Our goal is to identify relevant and preferably beta-specific markers to assess the activation of beta-cell stress and apoptotic mechanisms, and therefore the general quality of the islet preparation prior to transplantation. Protein expression and activation were analyzed by Western blotting and kinase assays. ATP measurements were performed by a luminescence-based assay. Oxygen consumption rate (OCR was measured based on standard protocols using fiber optic sensors. Total RNA was used for gene expression analyzes. Our results indicate that pancreas digestion initiates a potent stress response in the islets by activating two stress kinases, c-Jun N-terminal Kinase (JNK and p38. JNK1 protein levels remained unchanged between different islet preparations and following culture. In contrast, levels of JNK3 increased after islet culture, but varied markedly, with a subset of preparations bearing low JNK3 expression. The observed changes in JNK3 protein content strongly correlated with OCR measurements as determined by the Spearman's rank correlation coefficient rho [Formula: see text] in the matching islet samples, while inversely correlating with c-fos mRNA expression [Formula: see text]. In conclusion, pancreas digestion recruits JNK and p38 kinases that are known to participate to beta-cell apoptosis. Concomitantly, the islet isolation alters JNK3 and c-fos expression, both strongly correlating with OCR. Thus, a comparative analysis of JNK3 and c-fos expression before and after culture may provide for novel markers to assess islet quality prior to transplantation. JNK3 has the advantage over all other proposed markers to be islet-specific, and thus to provide for a marker independent of non-beta cell contamination.

  10. Individual variations in maternal care early in life correlate with later life decision-making and c-fos expression in prefrontal subregions of rats.

    Directory of Open Access Journals (Sweden)

    Felisa N van Hasselt

    Full Text Available Early life adversity affects hypothalamus-pituitary-adrenal axis activity, alters cognitive functioning and in humans is thought to increase the vulnerability to psychopathology--e.g. depression, anxiety and schizophrenia--later in life. Here we investigated whether subtle natural variations among individual rat pups in the amount of maternal care received, i.e. differences in the amount of licking and grooming (LG, correlate with anxiety and prefrontal cortex-dependent behavior in young adulthood. Therefore, we examined the correlation between LG received during the first postnatal week and later behavior in the elevated plus maze and in decision-making processes using a rodent version of the Iowa Gambling Task (rIGT. In our cohort of male and female animals a high degree of LG correlated with less anxiety in the elevated plus maze and more advantageous choices during the last 10 trials of the rIGT. In tissue collected 2 hrs after completion of the task, the correlation between LG and c-fos expression (a marker of neuronal activity was established in structures important for IGT performance. Negative correlations existed between rIGT performance and c-fos expression in the lateral orbitofrontal cortex, prelimbic cortex, infralimbic cortex and insular cortex. The insular cortex correlations between c-fos expression and decision-making performance depended on LG background; this was also true for the lateral orbitofrontal cortex in female rats. Dendritic complexity of insular or infralimbic pyramidal neurons did not or weakly correlate with LG background. We conclude that natural variations in maternal care received by pups may significantly contribute to later-life decision-making and activity of underlying brain structures.

  11. Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

    Directory of Open Access Journals (Sweden)

    Kun-Yuan Chiu


    Full Text Available Antcin-H, a natural triterpene, is purified from a famous anticancer medicinal mushroom, Antrodia cinnamomea, in Taiwan. This study showed that antcin-H inhibited the growth of human renal carcinoma 786-0 cells; the IC50 value (for 48 h was 170 μM. Besides, the migration and invasion of 786-0 cells were suppressed by antcin-H under noncytotoxic concentrations (<100 μM; these events were accompanied by inhibition of FAK and Src kinase activities, decrease of paxillin phosphorylation, impairment of lamellipodium formation, and upregulation of TIMPs and downregulation of MMPs, especially MMP-7 expression. Luciferase reporter assay showed that antcin-H repressed the MMP-7 promoter activity, in parallel to inhibiting c-Fos/AP-1 and C/EBP-β transactivation abilities. Moreover, antcin-H suppressed the activity of ERK1/2 and decreased the binding ability of C/EBP-β and c-Fos on the upstream/enhancer region of MMP-7 promoter. Overall, this study demonstrated that the anti-invasive effect of antcin-H in human renal carcinoma 786-0 cells might be at least in part by abrogating focal adhesion complex and lamellipodium formation through inhibiting the Src/FAK-paxillin signaling pathways and decreasing MMP-7 expression through suppressing the ERK1/2-AP-1/c-Fos and C/EBP-β signaling axis. Our findings provide the evidence that antcin-H may be an active component existing in A. cinnamomea with anticancer effect.

  12. A c-fos-monomeric red fluorescent protein 1 fusion transgene is differentially expressed in rat forebrain and brainstem after chronic dehydration and rehydration. (United States)

    Yoshimura, M; Ohkubo, J; Katoh, A; Ohno, M; Ishikura, T; Kakuma, T; Yoshimatsu, H; Murphy, D; Ueta, Y


    We have previously shown that an acute osmotic stimulation induces the expression of a c-fos and monomeric red fluorescent protein 1 (mRFP1) fusion transgene in osmosensitive rat brain areas, including the supraoptic (SON) and paraventricular nuclei (PVN). However, the effects of chronic stimuli, such as dehydration, have not been investigated. In the present study, the expression patterns of the c-fos-mRFP1 fusion gene in the forebrain and the brainstem of male and female transgenic rats were studied in seven experimental groups: ad lib. water (euhydration), water deprivation for 12, 24 or 48 h (dehydration) and water deprivation for 46 h + ad lib. water for 2, 6 or 12 h (rehydration). The number of cells that express nuclear mRFP1 fluorescence was quantified in the hypothalamus, the circumventricular organs and the brainstem. Compared to the euhydrated state, the number of transgene expressing cells significantly increased in all forebrain areas and in the rostral ventrolateral medulla after dehydration and 2 h of rehydration. In the nucleus of the solitary tract and area postrema, the number of mRFP1 fluorescent cells was markedly increased after 2 h of rehydration. Although the number of mRFP1 fluorescent cells in the organum vasculosum laminae terminalis, median preoptic nucleus and subfornical organ remained significantly increased after 6 h of rehydration, reaching control levels after 12 h of rehydration, the number of mRFP1 fluorescent cells in the SON and the PVN reached control levels after 6 h of rehydration. There were no significant differences between male and female rats. These results show that the expression of the c-fos-mRFP1 fusion gene changes in the forebrain and the brainstem not only after acute osmotic stimulation, but also after chronic osmotic stimulation. Interestingly, these studies reveal the differential activation of different neuronal groups over the time course of dehydration and rehydration. © 2013 British Society for

  13. Differential cortical c-Fos and Zif-268 expression after object and spatial memory processing in a standard or episodic-like object recognition task

    Directory of Open Access Journals (Sweden)

    Flávio F Barbosa


    Full Text Available Episodic memory reflects the capacity to recollect what, where and when a specific event happened in an integrative manner. Animal studies have suggested that the medial temporal lobe and the medial pre-frontal cortex are important for episodic-like memory formation. The goal of present study was to evaluate whether there are different patterns of expression of the immediate early genes c-Fos and Zif-268 in these cortical areas after rats are exposed to object recognition tasks with different cognitive demands. Male rats were randomly assigned to five groups: home cage control (CTR-HC, empty open field (CTR-OF, open field with one object (CTR-OF + Obj, novel object recognition task (OR and episodic-like memory task (ELM and were killed one hour after the last behavioral procedure. Rats were able to discriminate the objects in the OR task. In the ELM task, rats showed spatial (but not temporal discrimination of the objects. We found an increase in the c-Fos expression in the dorsal dentate gyrus (DG and in the perirhinal cortex (PRh in the OR and ELM groups. The OR group also presented an increase of c-Fos expression in the medial prefrontal cortex (mPFC. Additionally, the OR and ELM groups had increased expression of Zif-268 in the mPFC. Moreover, Zif-268 was increased in the dorsal CA1 and perirhinal cortex only in the ELM group. In conclusion, the pattern of activation was different in tasks with different cognitive demands. Accordingly, correlation tests suggest the engagement of different neural networks in the object recognition tasks used. Specifically, perirhinal-dentate gyrus co-activation was detected after the what-where memory retrieval, but not after the novel object recognition task. Both regions correlated with the respective behavioral outcome. These findings can be helpful in the understanding of the neural networks underlying memory tasks with different cognitive demands.

  14. Neuroanatomical Relationships between Orexin/Hypocretin-Containing Neurons/Nerve Fibers and Nicotine-Induced c-Fos-Activated Cells of the Reward-Addiction Neurocircuitry (United States)

    Dehkordi, Ozra; Rose, Jed E; Dávila-García, Martha I; Millis, Richard M; Mirzaei, Samar Ali; Manaye, Kebreten F; Jayam-Trouth, Annapurni


    Orexin/hypocretin-containing neurons in lateral hypothalamus (LH) are implicated in the neurobiology of nicotine addiction. However, the neuroanatomical relationships between orexin-neurons/nerve fibers and nicotine-activated cells within the reward-addiction neurocircuitry is not known. In the present study in mice, we first used c-Fos immunohistochemistry to identify CNS cells stimulated by an acute single injection of nicotine (NIC, 2 mg/kg, IP). Sequential double-labelling was then performed to identify the location of orexin-containing neurons and nerve fibers with respect to NIC-induced c-Fos activated cells and/or tyrosine hydroxylase (TH) immunoreactive (IR) cells of the mesocorticolimbic reward-addiction pathways. Orexin-IR nerve fibers and terminals were detected at multiple sites of the NIC reward-addiction circuitry in close apposition to, and intermingled with, NIC-induced c-Fos-IR cells of locus coeruleus (LC), ventral tegmental area (VTA), nucleus accumbens (Acb), LH and paraventricular thalamic nucleus (PVT). Double-labelling of orexin with TH showed frequent contact between orexin-IR nerve fibers and noradrenergic cells of LC. However, there was infrequent contact between the orexinergic fibers and the TH-expressing dopaminergic cells of VTA, dorsal raphe nucleus (DR), posterior hypothalamus (DA11), arcuate hypothalamic nucleus (DA12) and periventricular areas (DA14). The close anatomical contact between orexinergic nerve fibers and NIC-activated cells at multiple sites of the reward-addiction pathways suggests that orexinergic projections from LH are likely to be involved in modulating activity of the neurons that are directly impacted by acute administration of nicotine. PMID:29038792

  15. Beta-adrenoceptor Activation by Norepinephrine Enhances Lipopolysaccharide-induced Matrix Metalloproteinase-9 Expression Through the ERK/JNK-c-Fos Pathway in Human THP-1 Cells (United States)

    Yin, Xiang; Zhou, Linli; Han, Fei; Han, Jie; Zhang, Yuanyuan; Sun, Zewei; Zhao, Wenting; Wang, Zhen


    Aim: Atherosclerosis is a chronic inflammatory disease, which leads to thrombosis and acute coronary syndrome. Matrix metalloproteinase-9 (MMP-9) is involved in the stability of the extracellular matrix (ECM) and atherosclerosis plaque. Until now, it is established that lipopolysaccharide (LPS) and norepinephrine (NE) are associated with the pathological process of atherosclerosis. However, the combined effect of LPS and NE on MMP-9 is unclear. We investigated the combined effect of LPS and NE on MMP-9 expression in human monocytes and the mechanism involved in the process. Methods: THP-1 cells were cultured and treated with LPS and/or NE. MMP-9 and TIMP-1 gene and protein expression were detected by real time PCR and ELISA, respectively. MMP-9 activity was detected by gelatin zymography. Adrenoceptor antagonists and MAPKs inhibitors were used to clarify the mechanism. Pathway-related proteins were detected by Western blot. Results: We found that NE enhances LPS-induced MMP-9 and TIMP-1 expression as well as MMP-9 activity in THP-1 cells. This effect is reversed by the beta (β)-adrenoceptor antagonist propranolol, extracellular signal-regulated kinases (ERK) inhibitor U0126, and c-Jun N-terminal kinase (JNK) inhibitor SP600125. NE enhances LPS-induced ERK/JNK phosphorylation. NE up-regulates LPS-induced c-Fos expression, which is counteracted by propranolol, U0126, and SP600125. Furthermore, c-Fos silence reverses the effect of NE on MMP-9 activity. Conclusions: Our results suggest that NE enhances LPS-induced MMP-9 expression through β-adrenergic receptor and downstream ERK/JNK-c-Fos pathway. This study may help us to understand the combined effect and mechanism of NE/LPS on MMP-9 expression. PMID:27237101

  16. c-myc, c-fos, and c-jun regulation in the regenerating livers of normal and H-2K/c-myc transgenic mice.


    Morello, D; Fitzgerald, M J; Babinet, C; Fausto, N


    We investigated the mechanisms of regulation of c-myc, c-fos, and c-jun at the early stages of liver regeneration in mice. We show that the transient increase in steady-state levels of c-myc mRNA at the start of liver regeneration is most probably regulated by posttranscriptional mechanisms. Although there was a marked increase in c-myc transcriptional initiation shortly after partial hepatectomy, a block in elongation prevented the completion of most transcripts. To gain further information ...

  17. Expression of c-Fos in the rat retrosplenial cortex during instrumental re-learning of appetitive bar-pressing depends on the number of stages of previous training

    Directory of Open Access Journals (Sweden)

    Olga E. Svarnik


    Full Text Available Learning is known to be accompanied by induction of c-Fos expression in cortical neurons. However, not all neurons are involved in this process. What the c-Fos expression pattern depends on is still unknown. In the present work we studied whether and to what degree previous animal experience about Task 1 influenced neuronal c-Fos expression in the retrosplenial cortex during acquisition of Task 2. Animals were progressively shaped across days to bar-press for food at the left side of the experimental chamber (Task 1. This appetitive bar-pressing behavior was shaped by nine stages ("9 stages" group, five stages ("5 stages" group or one intermediate stage ("1 stage" group. After all animals acquired the first skill and practiced it for five days, the bar and feeder on the left, familiar side of the chamber were inactivated, and the animals were allowed to learn a similar instrumental task at the opposite side of the chamber using another pair of a bar and a feeder (Task 2. The highest number of c-Fos positive neurons was found in the retrosplenial cortex of "1 stage" animals as compared to the other groups. The number of c-Fos positive neurons in "5 stages" group animals was significantly lower than in "1 stage" animals and significantly higher than in "9 stages" animals. The number of c-Fos positive neurons in the cortex of "9 stages" animals was significantly higher than in home caged control animals. At the same time, there were no significant differences between groups in such behavioral variables as the number of entrees into the feeder or bar zones during Task 2 learning. Our results suggest that c-Fos expression in the retrosplenial cortex during Task 2 acquisition was influenced by the previous learning history.

  18. Changes of calcium binding proteins, c-Fos and COX in hippocampal formation and cerebellum of Niemann-Pick, type C mouse. (United States)

    Byun, Kyunghee; Kim, Daesik; Bayarsaikhan, Enkhjaigal; Oh, Jeehyun; Kim, Jisun; Kwak, Grace; Jeong, Goo-Bo; Jo, Seung-Mook; Lee, Bonghee


    Niemann-Pick disease, type C (NPC) is an intractable disease that is accompanied by ataxia, dystonia, neurodegeneration, and dementia due to an NPC gene defect. Disruption of calcium homeostasis in neurons is important in patients with NPC. Thus, we used immunohistochemistry to assess the expression levels of calcium binding proteins (calbindin D28K, parvalbumin, and calretinin), c-Fos and cyclooxygenase-1,2 (COX-1,2) in the hippocampal formation and cerebellum of 4 and 8 week old NPC+/+, NPC+/-, and NPC-/- mice. General expression of these proteins decreased in the hippocampus and cerebellum of NPC-/- compared to that in both young and adult NPC+/+ or NPC+/- mice. Parvalbumin, COX-1,2 or c-Fos-immunoreactive neurons were widely detected in the CA1, CA3, and DG of the hippocampus, but the immunoreactivities were decreased sharply in all areas of hippocampus of NPC-/- compared to NPC+/+ and NPC+/- mice. Taken together, reduction of these proteins may be one of the strong phenotypes related to the neuronal degeneration in NPC-/- mice. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. Repeated exposure to cat urine induces complex behavioral, hormonal, and c-fos mRNA responses in Norway rats ( Rattus norvegicus) (United States)

    Yin, Baofa; Gu, Chen; Lu, Yi; Hegab, Ibrahim M.; Yang, Shengmei; Wang, Aiqin; Wei, Wanhong


    Prey species show specific adaptations that allow recognition, avoidance, and defense against predators. This study was undertaken to investigate the processing of a chronic, life-threatening stimulus to Norway rats ( Rattus norvegicus). One hundred forty-four Norway rats were tested by repeated presentation of cat urine for 1 h at different days in a defensive withdrawal apparatus. Rats exposed to urine for short periods showed significantly larger defensive behavioral and medial hypothalamic c-fos messenger RNA (mRNA) responses than other groups. These defensive responses habituated shortly after the presentation of cat urine. Serum levels of adrenocorticotropic hormone and corticosterone increased significantly when animals were repeatedly exposed to cat urine. However, the hormonal responses took longer to habituate than the behavioral and molecular responses did. We conclude that the behavioral and c-fos mRNA responses are "primed" for habituation to repeated exposures to cat urine, while the hormonal responses show "resistance." The results support our hypothesis that the strongest anti-predator responses at three levels would occur during short-term exposure to cat urine and that these responses would subsequently disappear on prolonged exposure. This study assists understanding the way in which the different levels of defensive responses are integrated and react during chronic stress.

  20. Dynamic acetylation of all lysine 4-methylated histone H3 in the mouse nucleus: analysis at c-fos and c-jun.

    Directory of Open Access Journals (Sweden)

    Catherine A Hazzalin


    Full Text Available A major focus of current research into gene induction relates to chromatin and nucleosomal regulation, especially the significance of multiple histone modifications such as phosphorylation, acetylation, and methylation during this process. We have discovered a novel physiological characteristic of all lysine 4 (K4-methylated histone H3 in the mouse nucleus, distinguishing it from lysine 9-methylated H3. K4-methylated histone H3 is subject to continuous dynamic turnover of acetylation, whereas lysine 9-methylated H3 is not. We have previously reported dynamic histone H3 phosphorylation and acetylation as a key characteristic of the inducible proto-oncogenes c-fos and c-jun. We show here that dynamically acetylated histone H3 at these genes is also K4-methylated. Although all three modifications are proven to co-exist on the same nucleosome at these genes, phosphorylation and acetylation appear transiently during gene induction, whereas K4 methylation remains detectable throughout this process. Finally, we address the functional significance of the turnover of histone acetylation on the process of gene induction. We find that inhibition of turnover, despite causing enhanced histone acetylation at these genes, produces immediate inhibition of gene induction. These data show that all K4-methylated histone H3 is subject to the continuous action of HATs and HDACs, and indicates that at c-fos and c-jun, contrary to the predominant model, turnover and not stably enhanced acetylation is relevant for efficient gene induction.

  1. Do similar neural systems subserve aggressive and sexual behaviour in male rats? Insights from c-Fos and pharmacological studies

    NARCIS (Netherlands)

    Veening, Jan G.; Coolen, LM; de Jong, TR; Joosten, Henk W.; Koolhaas, Jaap M.; Olivier, B; Coolen, Lique M.; Boer, Sietze F. de


    It is a common belief that male aggressive and sexual behaviour share many of the underlying neurobiological, neurological, pharmacological and neuroendocrine mechanisms. Therefore, we studied brain activation patterns in male rat after performance of aggressive and sexual behaviour and compared

  2. Prolonged induction of c-fos in neuropeptide Y- and somatostatin-immunoreactive neurons of the rat dentate gyrus after electroconvulsive stimulation

    DEFF Research Database (Denmark)

    Woldbye, D P; Greisen, M H; Bolwig, T G


    RNA and Fos was observed. Compared to the granular layer, however, c-fos mRNA and Fos in hilar cells reached maximum later and remained elevated considerably longer. Several neurochemically distinct populations of hilar neurons have been described, some of which contain neuropeptide Y (NPY) and....../or somatostatin (SS). Using double-labelling immunocytochemistry, we examined to what extent Fos was induced in these hilar neurons after ECS. Although a minor population of non-NPY non-SS cells displayed Fos induction early after ECS, prolonged induction of Fos almost exclusively occurred in NPY or SS neurons....... The Fos-immunoreactive NPY or SS neurons only amounted to about 50% of the total hilar population of NPY or SS neurons. The present observations suggest that a subpopulation of hilar NPY and SS neurons may be central to the actions of electroconvulsive seizures in the dentate gyrus....

  3. Influence of Pre-reproductive Maternal Enrichment on Coping Response to Stress and Expression of c-Fos and Glucocorticoid Receptors in Adolescent Offspring. (United States)

    Cutuli, Debora; Berretta, Erica; Pasqualini, Greta; De Bartolo, Paola; Caporali, Paola; Laricchiuta, Daniela; Sampedro-Piquero, Patricia; Gelfo, Francesca; Pesoli, Matteo; Foti, Francesca; Begega, Azucena; Petrosini, Laura


    Environmental enrichment (EE) is an experimental setting broadly used for investigating the effects of complex social, cognitive, and sensorimotor stimulations on brain structure and function. Recent studies point out that parental EE experience, even occurring in the pre-reproductive phase, affects neural development and behavioral trajectories of the offspring. In the present study we investigated the influences of pre-reproductive EE of female rats on maternal behavior and adolescent male offspring's coping response to an inescapable stressful situation after chronic social isolation. For this purpose female Wistar rats were housed from weaning to breeding age in enriched or standard environments. Subsequently, all females were mated and housed in standard conditions until offspring weaning. On the first post partum day (ppd 1), mother-pup interactions in undisturbed conditions were recorded. Further, after weaning the male pups were reared for 2 weeks under social isolation or in standard conditions, and then submitted or not to a single-session Forced Swim Test (FST). Offspring's neuronal activation and plastic changes were identified by immunohistochemistry for c-Fos and glucocorticoid receptors (GRs), and assessed by using stereological analysis. The biochemical correlates were measured in the hippocampus, amygdala and cingulate cortex, structures involved in hypothalamic-pituitary-adrenocortical axis regulation. Enriched dams exhibited increased Crouching levels in comparison to standard reared dams. In the offspring of both kinds of dams, social isolation reduced body weight, decreased Immobility, and increased Swimming during FST. Moreover, isolated offspring of enriched dams exhibited higher levels of Climbing in comparison to controls. Interestingly, in the amygdala of both isolated and control offspring of enriched dams we found a lower number of c-Fos immunopositive cells in response to FST and a higher number of GRs in comparison to the offspring of

  4. Reactive Oxygen Species-Dependent c-Fos/Activator Protein 1 Induction Upregulates Heme Oxygenase-1 Expression by Bradykinin in Brain Astrocytes. (United States)

    Hsieh, Hsi-Lung; Wang, Hui-Hsin; Wu, Cheng-Ying; Yang, Chuen-Mao


    Heme oxygenase-1 (HO-1) plays a crucial role in tissue pathological changes such as brain injuries. Our previous studies have demonstrated that bradykinin (BK) induces the expression of several inflammatory proteins, including matrix metalloproteinase-9 and COX-2, via mitogen-activated protein kinases and nuclear factor-κB (NF-κB) in rat brain astrocytes (RBA-1). However, the molecular mechanisms underlying BK-induced HO-1 expression in RBA-1 cells remain poorly defined. Here we demonstrated that BK induced HO-1 expression and enzymatic activity via a B(2) BK receptor-activated reactive oxygen species (ROS)-dependent signaling pathway. NADPH oxidase (Nox)-dependent ROS generation led to activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun-N-terminal kinase (JNK) and then activated the downstream molecules NF-κB and c-Jun, respectively. The c-Fos, an activator protein 1 (AP-1) subunit, was upregulated by activation of NF-κB and c-Jun, which bound to HO-1 promoter and thereby turned on transcription of HO-1 gene. The rat HO-1 promoter containing a putative AP-1 cis-binding site was identified as a crucial domain linking to BK action. Taken together, these results suggested that in RBA-1 cells, activation of ERK/NF-κB and JNK/c-Jun cascades by a Nox/ROS-dependent event enhancing c-Fos/AP-1 activity is essential for HO-1 upregulation and activation induced by BK. Moreover, ROS-dependent NF-E2-related factor 2 activation also contributes to HO-1 induction by BK in astrocytes.

  5. Extinction of conditioned taste aversion is related to the aversion strength and associated with c-fos expression in the insular cortex. (United States)

    Hadamitzky, M; Bösche, K; Engler, A; Schedlowski, M; Engler, H


    Taste aversion learning is a type of conditioning where animals learn to associate a novel taste (conditioned stimulus; CS) with a stimulus inducing symptoms of poisoning or illness (unconditioned stimulus; US). As a consequence animals later avoid this taste, a reaction known as conditioned taste aversion (CTA). An established CTA extinguishes over time when the CS is repeatedly presented in the absence of the US. However, inter-individual differences in CTA extinction do exist. Using a model of behavioral conditioning with saccharin as CS and the immunosuppressant cyclosporine A as US, the present study aimed at further elucidating the factors underlying individual differences in extinction learning by investigating whether extinction of an established CTA is related to the strength of the initially acquired CS-US association. In addition, we analyzed the expression of the neuronal activation marker c-fos in brain structures relevant for acquisition and retrieval of the CTA, such as the insular cortex and the amygdala. We here show that animals, displaying a strong CS-US association during acquisition, maintained a strong CTA during unreinforced CS re-exposures, in contrast to animals with moderate CS-US association. Moreover, the latter animals showed increased c-fos mRNA expression in the insular cortex. Our data indicate that CTA extinction apparently depends on the strength of the initially learned CS-US association. In addition, these findings provide further evidence that the memory for the initial excitatory conditioning and its subsequent extinction is probably stored in those structures that participate in the processing of the CS and the US. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

  6. Neurobehavioral effects, c-Fos/Jun expression and tissue distribution in rat offspring prenatally co-exposed to MeHg and PFOA: PFOA impairs Hg retention. (United States)

    Cheng, Jinping; Fujimura, Masatake; Zhao, Wenchang; Wang, Wenhua


    Exposure to methylmercury (MeHg) and perfluorooctanoic acid (PFOA) can occur simultaneously as both contaminants are found in the same food sources, especially fish, seafood, marine mammals and milk. The aim of this study was to assess the effects of exposure to MeHg (10 μg mL(-1) in drinking water) and PFOA (10 μg mL(-1) in drinking water) from gestational day 1 to postnatal day (PND) 21, alone and in combination, on neurobehavioral development and the expression of c-Fos/Jun in different brain regions in the offspring. Our findings showed that exposure to MeHg alone, and exposure to MeHg combined with PFOA significantly induced cliff avoidance reflexes and negative geotaxis reflexes. And these effects appeared to be greater following exposure to MeHg alone. MeHg and/or PFOA exposure did not significantly impair motor coordination functions, or cause significant changes in c-Fos expression in the hippocampus and cerebellum, and spatial learning tests were similar to those in the controls, thus it was impossible to determine whether combined exposure to MeHg and PFOA had any additional effects on both hippocampus and cerebellum regions. However, a significant increase in the frequency of line crossing was observed in rats treated with MeHg or PFOA alone, and there were no significant differences between the MeHg+PFOA-treated group and the controls, suggesting that PFOA was antagonistic to MeHg toxicity in the locomotor activity test. Co-exposure to MeHg and PFOA decreased all tissue Hg concentrations in pups compared to the group exposed to MeHg only, suggesting that PFOA impaired Hg retention in different tissues. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Structure of distribution gas network operation system; Structure du systeme d'exploitation du reseau de distribution du gaz

    Energy Technology Data Exchange (ETDEWEB)

    Dzirba, E. [Institute of Oil and Gas (Poland); Osiadacz, A. [Warsaw Technical Uniwersity (Poland)


    Distribution networks have become increasingly complex to manage and potentially labor-intensive to operate safely and efficiently. Remote monitoring enables the operator to continually monitor the performance of the system from a central point(s). Where the system operates outside known parameters, the operator can respond very quickly. Remote control provides the additional functionality of enabling plant to be operated from a central point(s) directly or indirectly via operator. Communication can be through one or a combination of different communication media. The whole system for operation of gas distribution network may be looked at as 5-level general model based on ISO proposals adapted for modern control and management systems. The structure and requirements imposed on such a system are described in this paper. (authors)

  8. Dizocilpine and cycloheximide prevent inhibition of c-Fos gene expression by delta sleep-inducing peptide in the paraventricular nucleus of the hypothalamus in rats with different resistance to emotional stress. (United States)

    Umriukhin, P E; Koplik, E V; Sudakov, K V


    The effects of the non-competitive NMDA-receptor blocker MK-801 (dizocilpine) and the protein synthesis inhibitor cycloheximide on the delta sleep-inducing peptide (DSIP) inhibition of c-Fos immediate early gene expression were studied in the parvocellular subdivision of the hypothalamic paraventricular nucleus (pPVN) of male Wistar rats with either high or low resistance to emotional stress, predicted from differences in their open-field behaviour. The experiments show that intraperitoneal (i.p.) DSIP injection (60 nmol/kg) decreased the number of Fos-immunoreactive (Fos-IR) cells in the pPVN, activated by immobilization. The NMDA-receptor antagonist dizocilpine (MK-801) (90 nmol i.c.v.) prevented the inhibition of c-Fos expression by DSIP in the pPVN of rats predisposed to emotional stress. The protein synthesis inhibitor cycloheximide (210 nmol i.c.v.) prevented the inhibition of c-Fos expression by DSIP in the pPVN of rats that were resistant to emotional stress. The experiments indicate that the DSIP effect on c-Fos gene expression might be mediated by NMDA-receptors. DSIP may induce production of some protein transcription factors, transmitting a signal from membrane NMDA-receptors to the nucleus. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  9. Morphine-induced conditioned place preference and the alterations of p-ERK, p-CREB and c-fos levels in hypothalamus and hippocampus: the effects of physical stress. (United States)

    Pahlevani, P; Fatahi, Z; Moradi, M; Haghparast, A


    The hypothalamus and hippocampus are important areas involved in stress responses and reward processing. In addition, ERK/CREB pathway plays a critical role in the control of cellular responses to stress and reward. In the current study, effects of acute and subchronic stress on the alteration of p-ERK, p-CREB and c-fos levels in the hypothalamus and hippocampus of saline- or morphine-treated animals during morphine-induced conditioned place preference (CPP) procedure were investigated. Male Wistar rats were divided into two saline- and morphine-treated supergroups. Each supergroup includes of control, acute stress and subchronic stress groups. In all of groups, the CPP procedure was done, afterward the alternation of p-ERK/ERK ratio, p-CREB/CREB ratio and c-fos level in the hypothalamus and hippocampus were estimated by Western blot analysis. The results indicated that in saline- or morphine-treated animals, p-ERK/ERK ratio, p-CREB/CREB ratio and c-fos level increased after application of acute and subchronic stress (except for p-ERK/ERK ratio in morphine-control group). Our findings revealed that in saline- or morphine-treated animals, acute and subcronic stress increased the p-ERK/ERK ratio, p-CREB/CREB ratio and c-fos level in the hypothalamus and hippocampus and this enhancement in morphine-treated animals, was more considerable than that in saline-treated animals.

  10. Effects of the adenosine A 2A antagonist KW 6002 (istradefylline) on pimozide-induced oral tremor and striatal c-Fos expression: comparisons with the muscarinic antagonist tropicamide. (United States)

    Betz, A J; Vontell, R; Valenta, J; Worden, L; Sink, K S; Font, L; Correa, M; Sager, T N; Salamone, J D


    Typical antipsychotic drugs, including haloperidol and pimozide, have been shown to produce parkinsonian motor effects such as akinesia and tremor. Furthermore, there is an antagonistic interaction between adenosine A(2A) and dopamine D(2) receptors in the basal ganglia, which is important for motor functions related to the production of parkinsonian symptoms. Several experiments were conducted to assess the effects of the selective adenosine A(2A) antagonist KW 6002 on both the motor and cellular effects of subchronic administration of pimozide. The motor test employed was tremulous jaw movements, which is used as a model of parkinsonian tremor. In addition, c-Fos expression in the ventrolateral neostriatum, which is the striatal area most associated with tremulous jaw movements, was used as a marker of striatal cell activity in animals that were tested in the behavioral experiments. Repeated administration of 1.0 mg/kg pimozide induced tremulous jaw movements and increased ventrolateral striatal c-Fos expression, while administration of 20.0 mg/kg of the atypical antipsychotic quetiapine did not. The tremulous jaw movements induced by pimozide were significantly reduced by co-administration of either the adenosine A(2A) antagonist KW 6002 or the muscarinic antagonist tropicamide. Pimozide-induced increases in ventrolateral striatal c-Fos expression were reduced by a behaviorally effective dose of KW 6002, but c-Fos expression in pimozide-treated rats was actually increased by tropicamide. These results indicate that two different drug manipulations that act to reduce tremulous jaw movements can have different effects on DA antagonist-induced c-Fos expression, suggesting that adenosine A(2A) antagonism and muscarinic receptor antagonism exert their motor effects by acting on different striatal circuits.

  11. Influence of Pre-reproductive Maternal Enrichment on Coping Response to Stress and Expression of c-Fos and Glucocorticoid Receptors in Adolescent Offspring

    Directory of Open Access Journals (Sweden)

    Debora Cutuli


    Full Text Available Environmental enrichment (EE is an experimental setting broadly used for investigating the effects of complex social, cognitive, and sensorimotor stimulations on brain structure and function. Recent studies point out that parental EE experience, even occurring in the pre-reproductive phase, affects neural development and behavioral trajectories of the offspring. In the present study we investigated the influences of pre-reproductive EE of female rats on maternal behavior and adolescent male offspring's coping response to an inescapable stressful situation after chronic social isolation. For this purpose female Wistar rats were housed from weaning to breeding age in enriched or standard environments. Subsequently, all females were mated and housed in standard conditions until offspring weaning. On the first post partum day (ppd 1, mother-pup interactions in undisturbed conditions were recorded. Further, after weaning the male pups were reared for 2 weeks under social isolation or in standard conditions, and then submitted or not to a single-session Forced Swim Test (FST. Offspring's neuronal activation and plastic changes were identified by immunohistochemistry for c-Fos and glucocorticoid receptors (GRs, and assessed by using stereological analysis. The biochemical correlates were measured in the hippocampus, amygdala and cingulate cortex, structures involved in hypothalamic-pituitary-adrenocortical axis regulation. Enriched dams exhibited increased Crouching levels in comparison to standard reared dams. In the offspring of both kinds of dams, social isolation reduced body weight, decreased Immobility, and increased Swimming during FST. Moreover, isolated offspring of enriched dams exhibited higher levels of Climbing in comparison to controls. Interestingly, in the amygdala of both isolated and control offspring of enriched dams we found a lower number of c-Fos immunopositive cells in response to FST and a higher number of GRs in comparison to

  12. The effects of a selective inhibitor of c-Fos/activator protein-1 on endotoxin-induced acute kidney injury in mice

    Directory of Open Access Journals (Sweden)

    Miyazaki Hiroyuki


    Full Text Available Abstract Background Sepsis has been identified as the most common cause of acute kidney injury (AKI in intensive care units. Lipopolysaccharide (LPS induces the production of several proinflammatory cytokines including tumor necrosis factor (TNF-alpha, a major pathogenetic factor in septic AKI. c-Fos/activator protein (AP-1 controls the expression of these cytokines by binding directly to AP-1 motifs in the cytokine promoter regions. T-5224 is a new drug developed by computer-aided drug design that selectively inhibits c-Fos/AP-1 binding to DNA. In this study, we tested whether T-5224 has a potential inhibitory effect against LPS-induced AKI, by suppressing the TNF-alpha inflammatory response and other downstream effectors. Methods To test this hypothesis, male C57BL/6 mice at 7 weeks old were divided into three groups (control, LPS and T-5224 groups. Mice in the control group received saline intraperitoneally and polyvinylpyrrolidone solution orally. Mice in the LPS group were injected intraperitoneally with a 6 mg/kg dose of LPS and were given polyvinylpyrrolidone solution immediately after LPS injection. In the T-5224 group, mice were administered T-5224 orally at a dose of 300 mg/kg immediately after LPS injection. Serum concentrations of TNF-alpha, interleukin (IL-1beta, IL-6 and IL-10 were measured by ELISA. Moreover, the expression of intercellular adhesion molecule (ICAM-1 mRNA in kidney was examined by quantitative real-time RT-PCR. Finally, we evaluated renal histological changes. Results LPS injection induced high serum levels of TNF-alpha, IL-1beta and IL-6. However, the administration of T-5224 inhibited the LPS-induced increase in these cytokine levels. The serum levels of IL-10 in the LPS group and T-5224 group were markedly elevated compared with the control group. T-5224 also inhibited LPS-induced ICAM-1 mRNA expression. Furthermore histological studies supported an anti-inflammatory role of T-5224. Conclusions In endotoxin

  13. Masculinization induced by neonatal exposure to PGE(2) or estradiol alters c-fos induction by estrous odors in adult rats. (United States)

    Nugent, Bridget M; Wright, Christopher L; Zup, Susan L; McCarthy, Margaret M


    Processing of relevant olfactory and pheromonal cues has long been known as an important process necessary for social and sexual behavior in rodents. Several nuclei that receive input from the vomeronasal projection pathway are involved in sexual behavior and show changes in immediate early gene expression after stimulation with a variety of sex-related stimuli. The nuclei in this pathway are sexually dimorphic due to the early patterning events induced by estradiol derived from testicular androgens, which developmentally defeminize and masculinize the brain and adult sexual behavior. Masculinization can be induced independently of estradiol via prostaglandin-E(2) (PGE(2)), and therefore assessed separately from defeminization. Here we examined the effects of brain defeminization and masculinization on neuronal response to sex-related odors using Fos, the protein product of the immediate early gene c-fos, as an indicator of activity. Female rat pups treated with a cyclooxygenase-2 inhibitor, to reduce PGE(2), plus estradiol, estradiol alone, and PGE(2) alone were exposed to estrous female odor as adults and the resulting Fos expression was examined in the medial amygdala, preoptic area, and ventromedial nucleus of the hypothalamus. Defeminized and/or masculinized females all showed patterns of Fos activity similar to control males and significantly different from control females. These results suggest that early exposure to estradiol and PGE(2) do not affect olfaction in females, but switch the activity pattern of sex-related nuclei in females to resemble that of males following exposure to sexually-relevant cues.

  14. Reduced basal and novelty-induced levels of activity-regulated cytoskeleton associated protein (Arc) and c-Fos mRNA in the cerebral cortex and hippocampus of APPswe/PS1ΔE9 transgenic mice

    DEFF Research Database (Denmark)

    Christensen, Ditte Z; Thomsen, Morten Skøtt; Mikkelsen, Jens D


    in APP/PS1ΔE9 compared to wild-type mice. Novelty exposure induced an increase in Arc and c-Fos mRNA in the medial prefrontal cortex (mPFC), parietal cortex, and hippocampal formation in both APP/PS1ΔE9 transgenic and wild-type mice. However, novelty-induced IEG expression did not reach the same levels...

  15. In vivo crypt surface hyperproliferation is decreased by butyrate and increased by deoxycholate in normal rat colon: associated in vivo effects on c-Fos and c-Jun expression. (United States)

    Velázquez, O C; Zhou, D; Seto, R W; Jabbar, A; Choi, J; Lederer, H M; Rombeau, J L


    Studies on colon carcinogenesis suggest that the short-chain fatty acid butyrate may be protective, whereas the secondary bile acid deoxycholate may promote tumor development. Crypt surface hyperproliferation is regarded as a biomarker of colon cancer risk and can be modulated in vitro by the differentiation inducer butyrate and the tumor promoter deoxycholate. We hypothesized that butyrate decreases and deoxycholate increases crypt surface proliferation in vivo and that these effects are mediated by changes in the expression of the protooncogenes c-Fos and c-Jun, which are known to regulate proliferation and differentiation. Twenty-five adult Sprague-Dawley rats underwent colonic isolation and 24-hour intraluminal instillation of 10 mmol/L sodium chloride, 10 mmol/ L sodium butyrate, or 10 mmol/L sodium deoxycholate. Proliferation of the whole crypt and five crypt compartments from base to surface was assessed by proliferating cell nuclear antigen immunohistochemistry. The øh value, an index of "premalignant" hyperproliferation, was calculated as the ratio of labeled cells in the two surface compartments divided by the labeled cells in the entire crypt. Expression of c-Fos and c-Jun was evaluated by Western blot. Crypt surface proliferation and the øh value were significantly decreased by butyrate and increased by deoxycholate. Butyrate increased colonic expression of c-Jun, whereas deoxycholate significantly induced c-Fos. The in vivo effects on surface proliferation are consistent with a potential protective [corrected] role for butyrate and a promotive role for deoxycholate in colon carcinogenesis. The concurrently observed effects on colonic c-Jun and c-Fos expression represent a novel finding and suggest that direct or indirect modulation of protooncogene expression may be the mechanism by which these dietary byproducts regulate proliferation in vivo.

  16. Expression of c-Fos protein in medial septum/diagonal band of Broca and CA3 region, associated with the temporary inactivation of the supramammillary area. (United States)

    Aranda, Lourdes


    The supramammillary (SuM) area is part of the diencephalic nuclei comprising the mammillary bodies, and is a key structure in the memory and spatial learning processes. It is a critical region in the modulation/generation of hippocampal theta rhythm. In addition, many papers have recently shown a clear involvement of this structure in the processes of spatial learning and memory in animal models, although it is still not known how it modulates spatial navigation and response emotional. The aim of the present research was to study the effect of the temporary inactivation of the SuM area on synaptic plasticity of crucial structures in the formation of spatial memory and emotional response. Sprague-Dawley rats were asigned in three groups: a control group where the animals were not subjected to any treatment, and two groups where the rats received microinjections of tetrodotoxin (TTX) in the SuM area (5ng diluted in 0.5μl of saline) or saline (0.5μl). The microinjections were administered 90min before the perfusion. Later, cellular activity in medial septum/diagonal band of Broca (MS/DBB) and CA3 region of the dorsal hippocampus was assessed, by measuring the immediate early gene c-fos. The results show a clear hiperactivity cellular in medial septum/diagonal band of Broca and a clear hypoactivity cellular in the CA3 region of the hippocampus when there was a functional inactivation of the SuM area. It suggests that the SuM area seems to be part of the connection and information input pathways to CA3 region of the hippocampal formation, key for proper functioning in spatial memory and emotional response. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Anxiety- and depressive-like responses and c-fos activity in preproenkephalin knockout mice: oversensitivity hypothesis of enkephalin deficit-induced posttraumatic stress disorder. (United States)

    Kung, Jen-Chuang; Chen, Tsung-Chieh; Shyu, Bai-Chuang; Hsiao, Sigmund; Huang, Andrew Chih Wei


    The present study used the preproenkephalin knockout (ppENK) mice to test whether the endogenous enkephalins deficit could facilitate the anxiety- and depressive-like symptoms of posttraumatic stress disorder (PTSD). On Day 1, sixteen wildtype (WT) and sixteen ppENK male mice were given a 3 mA or no footshock treatment for 10 seconds in the footshock apparatus, respectively. On Days 2, 7, and 13, all mice were given situational reminders for 1 min per trial, and the freezing response was assessed. On Day 14, all mice were tested in the open field test, elevated plus maze, light/dark avoidance test, and forced swim test. Two hours after the last test, brain tissues were stained to examine c-fos expression in specific brain areas. The present results showed that the conditioned freezing response was significant for different genotypes (ppENK vs WT). The conditioned freezing effect of the ppENK mice was stronger than those of the WT mice. On Day 14, the ppENK mice showed more anxiety- and depressive-like responses than WT mice. The magnitude of Fos immunolabeling was also significantly greater in the primary motor cortex, bed nucleus of the stria terminalis-lateral division, bed nucleus of the stria terminalis-supracapsular division, paraventricular hypothalamic nucleus-lateral magnocellular part, central nucleus of the amygdala, and basolateral nucleus of the amygdala in ppENK mice compared with WT mice. In summary, animals with an endogenous deficit in enkephalins might be more sensitive to PTSD-like aversive stimuli and elicit stronger anxiety and depressive PTSD symptoms, suggesting an oversensitivity hypothesis of enkephalin deficit-induced PTSD.

  18. Masculinization induced by neonatal exposure to PGE2 or estradiol alters c-fos induction by estrous odors in adult rats (United States)

    Nugent, Bridget M.; Wright, Christopher L.; Zup, Susan L.; McCarthy, Margaret M.


    Processing of relevant olfactory and pheromonal cues has long been known as an important process necessary for social and sexual behavior in rodents. Several nuclei that receive input from the vomeronasal projection pathway are involved in sexual behavior and show changes in immediate early gene expression after stimulation with a variety of sex-related stimuli. The nuclei in this pathway are sexually dimorphic due to the early patterning events induced by estradiol derived from testicular androgens, which developmentally defeminize and masculinize the brain and adult sexual behavior. Masculinization can be induced independently of estradiol via prostaglandin-E2.(PGE2), and therefore assessed separately from defeminization. Here we examined the effects of brain defeminization and masculinization on neuronal response to sex-related odors using Fos, the protein product of the immediate early gene c-fos, as an indicator of activity. Female rat pups treated with a cyclooxygenase-2 inhibitor, to reduce PGE2, plus estradiol, estradiol alone, and PGE2 alone were exposed to estrous female odor as adults and the resulting Fos expression was examined in the medial amygdala, preoptic area, and ventromedial nucleus of the hypothalamus. Defeminized and/or masculinized females all showed patterns of Fos activity similar to control males and significantly different from control females. These results suggest that early exposure to estradiol and PGE2 do not affect olfaction in females, but switch the activity pattern of sex-related nuclei in females to resemble that of males following exposure to sexually-relevant cues. PMID:18976678

  19. Amitriptyline up-regulates connexin43-gap junction in rat cultured cortical astrocytes via activation of the p38 and c-Fos/AP-1 signalling pathway. (United States)

    Morioka, N; Suekama, K; Zhang, F F; Kajitani, N; Hisaoka-Nakashima, K; Takebayashi, M; Nakata, Y


    Intercellular communication via gap junctions, comprised of connexin (Cx) proteins, allow for communication between astrocytes, which in turn is crucial for maintaining CNS homeostasis. The expression of Cx43 is decreased in post-mortem brains from patients with major depression. A potentially novel mechanism of tricyclic antidepressants is to increase the expression and functioning of gap junctions in astrocytes. The effect of amitriptyline on the expression of Cx43 and gap junction intercellular communication (GJIC) in rat primary cultured cortical astrocytes was investigated. We also investigated the role of p38 MAPK intracellular signalling pathway in the amitriptyline-induced expression of Cx43 and GJIC. Treatment with amitriptyline for 48 h significantly up-regulated Cx43 mRNA, protein and GJIC. The up-regulation of Cx43 was not monoamine-related since noradrenaline, 5-HT and dopamine did not induce Cx43 expression and pretreatment with α- and β-adrenoceptor antagonists had no effect. Intracellular signalling involved p38 MAPK, as amitriptyline significantly increased p38 MAPK phosphorylation and Cx43 expression and GJIC were significantly blocked by the p38 inhibitor SB 202190. Furthermore, amitriptyline-induced Cx43 expression and GJIC were markedly reduced by transcription factor AP-1 inhibitors (curcumin and tanshinone IIA). The translocation of c-Fos from the cytosol and the nucleus of cortical astrocytes was increased by amitriptyline, and this response was dependent on p38 activity. These findings indicate a novel mechanism of action of amitriptyline through cortical astrocytes, and further suggest that targeting this mechanism could lead to the development of a new class of antidepressants. © 2014 The British Pharmacological Society.

  20. Effects of the fatty acid amide hydrolase inhibitor URB597 on the sleep-wake cycle, c-Fos expression and dopamine levels of the rat. (United States)

    Murillo-Rodríguez, Eric; Vázquez, Edgar; Millán-Aldaco, Diana; Palomero-Rivero, Marcela; Drucker-Colin, René


    Our group has described previously that the endogenous cannabinoid anandamide induces sleep. The hydrolysis of this lipid involves the activity of the fatty acid amide hydrolase (FAAH), which additionally catalyzes the degradation of the satiety factor oleoylethanolamide and the analgesic-inducing lipid palmitoylethanolamide. It has been demonstrated that the inhibition of the FAAH by URB597 increases levels of anandamide, oleoylethanolamide and palmitoylethanolamide in the brain of rats. In order to determinate the physiological properties of the FAAH inhibition on the sleep modulation, we report the pharmacological effects on the sleep-wake cycle of the rat after i.c.v. administrations of URB597, oleoylethanolamide or palmitoylethanolamide (10, 20 microg/5 microl). Separate unilateral i.c.v. injections of 3 compounds during the lights-on period, increased wakefulness and decreased slow wave (SW) sleep in rats in a dose-dependent fashion. We additionally found out that, compared to controls, c-Fos immunoreactivity in hypothalamus and dorsal raphe nucleus was increased in rats that received URB597, oleoylethanolamide or palmitoylethanolamide (10, 20 microg/5 microl, i.c.v.). Next, we found that after an injection of the compounds, levels of dopamine were increased whereas extracellular levels of levodopa (l-DOPA) were decreased. These findings indicate that that inhibition of the FAAH, via URB597, modulates waking. These effects were mimicked separately by the administration of oleoylethanolamide or palmitoylethanolamide. The alertness induced by the compounds tested here activated wake-promoting brain regions and they also induced the release of dopamine. Our results suggest that FAAH activity as well as two molecules that are catalyzed by this enzyme, oleoylethanolamide and palmitoylethanolamide, participate in the regulation of the waking state. Alternative approaches to treat sleep disorders such as excessive somnolence might consider the use of the URB597

  1. Variabilite spatiale des rendements du riz en systeme de riziculture ...

    African Journals Online (AJOL)

    Une étude exploratoire a été menée de 2009 à 2013 en vue d'évaluer la faisabilité technique du Système de Riziculture Intensive (SRI) dans cinq localités de l'Office du Niger. Le protocole expérimental comprenait 3 traitements : pratique paysanne avec la dose recommandée de fumure minérale (T1) ; méthode SRI avec ...

  2. Systems of the future; Les systemes du futur

    Energy Technology Data Exchange (ETDEWEB)



    The world population growth will impact largely on the energy and electric power demand in the future. Facing the decrease of the hydrocarbons reserves, the international community decided to work together to develop a new generation of nuclear systems. In this context, coordinated researches are realized first with a short dated objective on the development of innovations for PWR type reactors and second with a middle dated on the development of new systems in an international framework (essentially Generation IV). Theses research programs are presented below. The first part is devoted to the different generation of reactors (I to IV) and to the third generation; the second part deals with the international framework of the researches, the french strategy and the european dimension. (A.L.B.)


    Directory of Open Access Journals (Sweden)



    Full Text Available Pour déterminer les fonctions thermodynamiques de mélange des phases liquides et solides du système (Mg,Sn, nous avons établi un programme qui permet l’optimisation de ces fonctions et la restitution du diagramme de phases. A partir des informations expérimentales dont on dispose, l’ensemble des grandeurs thermodynamiques de la phase liquide et de la solution solide a été développé sur la base orthogonale de Legendre. Le diagramme de phases restitué à partir des fonctions optimisées est en bon accord avec le diagramme établi expérimentalement.

  4. The c-Fos and c-Jun from Litopenaeus vannamei play opposite roles in Vibrio parahaemolyticus and white spot syndrome virus infection. (United States)

    Li, Chaozheng; Li, Haoyang; Wang, Sheng; Song, Xuan; Zhang, Zijian; Qian, Zhe; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo


    Growing evidence indicates that activator protein-1 (AP-1) plays a major role in stimulating the transcription of immune effector molecules in cellular response to an incredible array of stimuli, including growth factors, cytokines, cellular stresses and bacterial and viral infection. Here, we reported the isolation and characterization of a cDNA from Litopenaeus vannamei encoding the full-length c-Fos protein (named as Lvc-Fos). The predicted amino acid sequences of Lvc-Fos contained a basic-leucine zipper (bZIP) domain, which was characteristic of members of the AP-1 family. Immunoprecipitation and native-PAGE assays determined that Lvc-Fos could interact with the Lvc-Jun, a homolog of c-Jun family in L. vannamei, in a heterodimer manner. Further investigation demonstrated that Lvc-Fos and Lvc-Jun were expressed in all tested tissues and located in the nucleus. Real-time RT-PCR analysis showed both Lvc-Fos and Lvc-Jun in gills were up-regulated during Vibrio parahaemolyticus and white spot syndrome virus (WSSV) challenges. In addition, reporter gene assays indicated Lvc-Fos and Lvc-Jun could activate the expression of antimicrobial peptides (AMPs) of Drosophila and shrimp, as well as WSSV immediate early (IE) genes wsv069 and wsv249, in a different manner. Knockdown of Lvc-Fos or Lvc-Jun by RNA interference (RNAi) resulted in higher mortalities of L. vannamei after infection with V. parahaemolyticus, suggesting that Lvc-Fos and Lvc-Jun might play protective roles in bacterial infection. However, silencing of Lvc-Fos or Lvc-Jun in shrimp caused lower mortalities and virus loads under WSSV infection, suggesting that Lvc-Fos and Lvc-Jun could be engaged for WSSV replication and pathogenesis. In conclusion, our results provided experimental evidence and novel insight into the roles of L. vannamei AP-1 in bacterial and viral infection. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Analyse comparative des systemes de production du coton ...

    African Journals Online (AJOL)

    Cet article fait une analyse économique de deux systèmes de production du coton au Bénin à savoir, le coton biologique et le coton conventionnel. Pour ce faire une brève description des caractéristiques technico-économiques de chacun des deux types de cultures a été présentée. En utilisant les données collectées lors ...


    African Journals Online (AJOL)


    croissance rapide et une bonne vigueur. (Obouayeba et al., 2002 ; Obouayeba, 2005) si bien qu'il était mis en saignée entre 4 et 4,5 ans. (Cirad, 1993). En outre, l'homogénéité du peuplement de ce clone en phase immature permet une saignée précoce de tous les arbres un an après l'ouverture. Son métabolisme actif,.

  7. Penilaian Kinerja Keuangan 5 Perusahaan Perbankan Terbesar Periode 2010-2012 Menggunakan DuPont System

    Directory of Open Access Journals (Sweden)

    Theresia Lesmana


    Full Text Available Assessment of corporate performance can be viewed from financial aspect and nonfinancial aspect. This study attempted specifically to measure financial performance by using the DuPont system of financial analysis. DuPont system disaggregates performance into three components. They are Net Profit Margin (NPM, Return on Assets (ROA and Return on Equity (ROE. Object of this study is five largest financial institutions based on market capitalization and go public. Those five financial institutions are Bank Republik Indonesia (Persero Tbk (BBRI, Bank Central Asia Tbk (BBCA, Bank Mandiri (Persero Tbk (BMRI, Bank Negara Indonesia (Persero Tbk (BBNI and Bank Danamon Indonesia Tbk (BDMN. The financial performance of five banks was measured for three periods, from 2010 until 2012. It was found that only Bank Negara Indonesia (Persero Tbk is the best financial performance using DuPont System.

  8. Brain Activation by H1 Antihistamines Challenges Conventional View of Their Mechanism of Action in Motion Sickness: A Behavioral, c-Fos and Physiological Study in Suncus murinus (House Musk Shrew

    Directory of Open Access Journals (Sweden)

    Longlong Tu


    Full Text Available Motion sickness occurs under a variety of circumstances and is common in the general population. It is usually associated with changes in gastric motility, and hypothermia, which are argued to be surrogate markers for nausea; there are also reports that respiratory function is affected. As laboratory rodents are incapable of vomiting, Suncus murinus was used to model motion sickness and to investigate changes in gastric myoelectric activity (GMA and temperature homeostasis using radiotelemetry, whilst also simultaneously investigating changes in respiratory function using whole body plethysmography. The anti-emetic potential of the highly selective histamine H1 receptor antagonists, mepyramine (brain penetrant, and cetirizine (non-brain penetrant, along with the muscarinic receptor antagonist, scopolamine, were investigated in the present study. On isolated ileal segments from Suncus murinus, both mepyramine and cetirizine non-competitively antagonized the contractile action of histamine with pKb values of 7.5 and 8.4, respectively; scopolamine competitively antagonized the contractile action of acetylcholine with pA2 of 9.5. In responding animals, motion (1 Hz, 4 cm horizontal displacement, 10 min increased the percentage of the power of bradygastria, and decreased the percentage power of normogastria whilst also causing hypothermia. Animals also exhibited an increase in respiratory rate and a reduction in tidal volume. Mepyramine (50 mg/kg, i.p. and scopolamine (10 mg/kg, i.p., but not cetirizine (10 mg/kg, i.p., significantly antagonized motion-induced emesis but did not reverse the motion-induced disruptions of GMA, or hypothermia, or effects on respiration. Burst analysis of plethysmographic-derived waveforms showed mepyramine also had increased the inter-retch+vomit frequency, and emetic episode duration. Immunohistochemistry demonstrated that motion alone did not induce c-fos expression in the brain. Paradoxically, mepyramine increased c-fos

  9. Spinal cord stimulation and the induction of c-fos and heat shock protein 72 in the central nervous system of rats

    NARCIS (Netherlands)

    DeJongste, MJL; Hautvast, RWM; Ruiters, MHJ; Ter Horst, GJ

    For more than a decade, spinal cord stimulation (SCS) has been used as an adjuvant treatment for patients who are unresponsive to conventional therapies for angina pectoris. Many studies showed that SCS has both electro-analgesic and anti-ischemic effects. Nonetheless, the biological substrates by

  10. Gonadotropin-releasing hormone (GnRH) agonist triptorelin inhibits estradiol-induced serum response element (SRE) activation and c-fos expression in human endometrial, ovarian and breast cancer cells. (United States)

    Gründker, Carsten; Günthert, Andreas R; Hellriegel, Martin; Emons, Günter


    The majority of human endometrial (>80%), ovarian (>80%) and breast (>50%) cancers express GnRH receptors. Their spontaneous and epidermal growth-factor-induced proliferation is dose- and time-dependently reduced by treatment with GnRH and its agonists. In this study, we demonstrate that the GnRH agonist triptorelin inhibits estradiol (E2)-induced cancer cell proliferation. The proliferation of quiescent estrogen receptor alpha (ER alpha)-/ER beta-positive, but not of ER alpha-negative/ER beta-positive endometrial, ovarian and breast cancer cell lines, was significantly stimulated (P<0.001) (ANOVA) after treatment with E2 (10(-8) M). This effect was time- and dose-dependently antagonized by simultaneous treatment with triptorelin. The inhibitory effect was maximal at 10(-5) M concentration of triptorelin (P<0.001). In addition, we could show that, in ER alpha-/ER beta-positive cell lines, E2 induces activation of serum response element (SRE) and expression of the immediate early-response gene c-fos. These effects were blocked by triptorelin (P<0.001). E2-induced activation of estrogen-response element (ERE) was not affected by triptorelin. The transcriptional activation of SRE by E2 is due to ER alpha activation of the mitogen-activated protein kinase (MAPK) pathway. This pathway is impeded by GnRH, resulting in a reduction of E2-induced SRE activation and, in consequence, a reduction of E2-induced c-fos expression. This causes downregulation of E2-induced cancer cell proliferation.

  11. Systemes De Recolte Du Latex Adaptes A La Saignee Descendante ...

    African Journals Online (AJOL)

    However systems applied up to now were to harvest the latex of this clone didn't allowed eradicating its sensitivity to tapping panel dryness and fragility. An experiment was led in south east of Côte d'Ivoire in order to determine a high performance harvesting technique of this clone in downward tapping. The clone PB 235 ...

  12. Geology and Petroleum Systems of the Offshore Benin Basin (Benin Géologie et système pétrolier du bassin offshore du Benin (Benin

    Directory of Open Access Journals (Sweden)

    Kaki C.


    Full Text Available This paper summarizes the tectonosedimentary development and petroleum system of the Offshore Benin Basin (OBB. In accordance with structural development, the stratigraphic succession of this basin was divided into 4 sequences: pre-rift (up to Late Jurassic; rift (Neocomian to Lower Cretaceous; transitional (Cenomanian to Santonian and post-rift (Maastrichtian-Holocene sequences. Only one Upper Cretaceous petroleum system is well known within this basin. Source rocks of this system contain Type II-III kerogens with a TOC (Total Organic Carbon average of 2.9%. Oil is produced from sandstone facies within Abeokuta formation. Currently exploration data and geochemical characteristics of bituminous sands which outcrop in some onshore areas of the Dahomey Embayment point to the existence of another petroleum system of Lower Cretaceous age (Neocomian to Albian in this basin. Cet article résume l’évolution tectono-sédimentaire et le système pétrolier du Bassin Offshore du Bénin (BOB. Conformément à l’évolution structurale, la succession stratigraphique de ce bassin a été divisée en quatre séquences : anté-rift (allant jusqu’à la fin du Jurassique; rift (Néocomien-Crétacé inférieur; transitionnelle (Cénomanien-Santonien et post-rift (Maastrichtien-Holocène. Un seul système pétrolier d’âge Crétacé supérieur a été identifié avec certitude dans ce bassin. Les roches mères de ce système contiennent du kérogène de Type II-III, avec un COT (Carbone Organique Total moyen de 2,9 %. L’huile est produite à partir des faciès gréseux de la formation d’Abeokuta. Les données actuelles d’exploration et les caractéristiques géochimiques des sables bitumineux qui affleurent par endroits dans la partie continentale de la baie du Dahomey mettent en évidence l’existence d’un autre système pétrolier d’âge Crétacé inférieur (Néocomien à Albien dans ce bassin.

  13. Potential Effect and Analysis of High Residential Solar Photovoltaic (PV Systems Penetration to an Electric Distribution Utility (DU

    Directory of Open Access Journals (Sweden)

    Jeffrey Tamba Dellosa


    Full Text Available The Renewable Energy Act of 2008 in the Philippines provided an impetus for residential owners to explore solar PV installations at their own rooftops through the Net-Metering policy. The Net-Metering implementation through the law however presented some concerns with inexperienced electric DU on the potential effect of high residential solar PV system installations. It was not known how a high degree of solar integration to the grid can possibly affect the operations of the electric DU in terms of energy load management. The primary objective of this study was to help the local electric DU in the analysis of the potential effect of high residential solar PV system penetration to the supply and demand load profile in an electric distribution utility (DU grid in the province of Agusan del Norte, Philippines. The energy consumption profiles in the year 2015 were obtained from the electric DU operating in the area. An average daily energy demand load profile was obtained from 0-hr to the 24th hour of the day based from the figures provided by the electric DU. The assessment part of the potential effect of high solar PV system integration assumed four potential total capacities from 10 Mega Watts (MW to 40 MW generated by all subscribers in the area under study at a 10 MW interval. The effect of these capacities were measured and analyzed with respect to the average daily load profile of the DU. Results of this study showed that a combined installations beyond 20 MWp coming from all subscribers is not viable for the local electric DU based on their current energy demand or load profile. Based from the results obtained, the electric DU can make better decisions in the management of high capacity penetration of solar PV systems in the future, including investment in storage systems when extra capacities are generated. Article History: Received July 15th 2016; Received in revised form Sept 23rd 2016; Accepted Oct 1st 2016; Available online How to Cite

  14. Paired-housing selectively facilitates within-session extinction of avoidance behavior, and increases c-Fos expression in the medial prefrontal cortex, in anxiety vulnerable Wistar-Kyoto rats. (United States)

    Smith, Ian M; Pang, Kevin C H; Servatius, Richard J; Jiao, Xilu; Beck, Kevin D


    The perseveration of avoidance behavior, even in the absence of once threatening stimuli, is a key feature of anxiety and related psychiatric conditions. This phenomenon can be observed in the Wistar-Kyoto (WKY) rat which, in comparison to outbred controls, demonstrates impaired extinction of avoidance behavior. Also characteristic of the WKY rat is abnormalities of the neurocircuitry and neuroplasticity of the medial prefrontal cortex (mPFC). One means of reducing physiological responses to anxiety, and conditioned fear, in social species is the presence of a conspecific animal. The current study investigates whether or not pair-housed WKY rats would show facilitated extinction of avoidance in comparison to individual-housed WKY rats, and whether or not pair-housing influences mPFC activation during lever-press avoidance. Male WKY rats were assigned to individual-housed and pair-housed conditions. Rats were trained in lever-press avoidance. Each session of lever-press avoidance consisted of 20 trials, where pressing a lever in response to a warning tone prevented foot-shocks. Rats received 12 acquisition sessions over 4weeks; followed by 6 extinction sessions over 2weeks, where foot-shocks ceased to be delivered. Brains were harvested 90min after trials 1 and 10 of extinction sessions 1 and 6, and mPFC sections underwent c-Fos staining as a measure of activation. Pair-housed rats showed facilitated lever-press avoidance extinction rates, but the main cause for this overall difference was a selective facilitation of within-session extinction. Similar to individual-housed rats, pair-housed rats continued to avoid during trial 1 of extinction even when the avoidance responding had been significantly reduced by the end of the previous session. Pair-housed rats sacrificed on trial 1 showed greater c-Fos expression in the anterior cingulate cortex and prelimbic cortex subregions of the mPFC compared individual-housed rats sacrificed on trial 1. This data shows pair

  15. A point mutation at tyrosine-809 in the human colony-stimulating factor 1 receptor impairs mitogenesis without abrogating tyrosine kinase activity, association with phosphatidylinositol 3-kinase, or induction of c-fos and junB genes

    Energy Technology Data Exchange (ETDEWEB)

    Roussel, M.F. (Univ. of Tennessee, Memphis (USA)); Shurtleff, S.A.; Downing, J.R. (Saint Jude Children' s Research Hospital, Memphis, TN (USA)); Sherr, C.J. (Univ. of Tennessee College of Medicine, Memphis (USA) Saint Jude Children' s Research Hospital, Memphis, TN (USA))


    Substitution of phenylalanine for tyrosine-809 in the human colony-stimulating factor 1 receptor (CSF-1R) inhibited its ability to transduce ligand-dependent mitogenic signals in mouse NIH 3T3 cells. When combined with an activating mutation at codon 301 that induces constitutive CSF-1R tyrosine kinase activity, the codon 809 mutation suppressed ligand-independent cell transformation. Comparative mapping tryptic phosphopeptides from mutant and wild-type CSF-1R indicated that tyrosine-809 is a site of ligand-dependent receptor phosphorylation in vivo. The mutant receptor was active as a tyrosine kinase in vitro and in vivo, underwent CSF-1-dependent association with a phosphatidylinositol 3-kinase, and induced expression of the protooncogenes c-fos and junB, underscoring its ability to trigger some of the known cellular responses to CSF-1. The mutant receptor is likely to be impaired in its ability to interact with critical cellular effectors whose activity is required for mitogenesis.

  16. Automatic control system in the reactor peggy; Systeme de pilotage automatique du reacteur peggy

    Energy Technology Data Exchange (ETDEWEB)

    Bertrand, J.; Mourchon, R.; Da Costa, D.; Desandre-Navarre, Ch. [Commissariat a l' Energie Atomique, Cadarache (France). Centre d' Etudes Nucleaires


    The equipment makes it possible for the reactor to attain a given power automatically and for the power to be maintained around this level. The principle of its operation consists in the changing from one power to another, at constant period, by means of a programmer transforming a power-step request into a voltage variation which is linear with time and which represents the logarithm of the required power. The real power is compared continuously with the required power. Stabilization occurs automatically as soon as the difference between the reactor power and the required power diminishes to a few per cent. (authors) [French] L'equipement permet au reacteur d'atteindre automatiquement une puissance affichee et de le stabiliser autour de cette puissance. Le principe du fonctionnement consiste a passer, a periode constante, d'une puissance a une autre, grace a un programmeur transformant une demande de puissance-echelon en une variation de tension lineaire en fonction du temps, tension representant le logarithme de la puissance affichee. La puissance reelle est comparee en permanence a la puissance affichee. La stabilisation intervient automatiquement lorsque la puissance du reacteur ne differe plus que de quelques pour cent de la valeur affichee. (auteurs)

  17. A peptide fragment of ependymin neurotrophic factor uses protein kinase C and the mitogen-activated protein kinase pathway to activate c-Jun N-terminal kinase and a functional AP-1 containing c-Jun and c-Fos proteins in mouse NB2a cells. (United States)

    Adams, David S; Hasson, Brendan; Boyer-Boiteau, Anne; El-Khishin, Adam; Shashoua, Victor E


    Ependymin (EPN) is a goldfish brain neurotrophic factor previously shown to function in a variety of cellular events related to long-term memory formation and neuronal regeneration. CMX-8933, an 8-amino-acid synthetic peptide fragment of EPN, was designed for aiding an investigation of the biological properties of this glycoprotein. We reported from previous studies that treatment of mouse neuroblastoma (NB2a) cultures with CMX-8933 promotes activation of transcription factor AP-1, a characteristic previously associated with the following full-length neurotrophic factors: nerve growth factor, neurotropin-3, and brain-derived neurotrophic factor. The CMX-8933-activated AP-1 specifically bound an AP-1 consensus probe and appeared to contain c-Jun and c-Fos protein components in antibody supershift experiments. Because AP-1 influences a variety of positive and negative cellular processes, determined in part by its exact protein composition and mechanism of activation, we extended these initial AP-1 observations in the current study to confirm the identity of the CMX-8933-activated c-Jun and c-Fos components. CMX-8933 increases the enzymatic activity of c-Jun N-terminal kinase (JNK), increases the phosphorylation of JNK and c-Jun proteins, and increases the cellular titers of c-Jun and c-Fos mRNAs. Furthermore, the AP-1 activated by CMX-8933 is functional, insofar as it transactivates both synthetic and natural AP-1-dependent reporter plasmids. Inhibition studies indicate that activation of the 8933-induced AP-1 occurs via the mitogen-activated protein kinase pathway. These data are in agreement with the recently proposed model for the conversion of short- to long-term synaptic plasticity and memory, in which a JNK-activated transcription factor AP-1, containing c-Jun and c-Fos components, functions at the top of a hierarchy of transcription factors known to regulate long-term neural plasticity. Copyright 2003 Wiley-Liss, Inc.

  18. Spatiotemporal differences in the c-fos pathway between C57BL/6J and DBA/2J mice following flurothyl-induced seizures: A dissociation of hippocampal Fos from seizure activity. (United States)

    Kadiyala, Sridhar B; Papandrea, Dominick; Tuz, Karina; Anderson, Tara M; Jayakumar, Sachidhanand; Herron, Bruce J; Ferland, Russell J


    Significant differences in seizure characteristics between inbred mouse strains highlight the importance of genetic predisposition to epilepsy. Here, we examined the genetic differences between the seizure-resistant C57BL/6J (B6) mouse strain and the seizure-susceptible DBA/2J (D2) strain in the phospho-Erk and Fos pathways to examine seizure-induced neuronal activity to uncover potential mechanistic correlates to these disparate seizure responsivities. Expression of neural activity markers was examined following 1, 5, or 8 seizures, or after 8 seizures, a 28 day rest period, and a final flurothyl rechallenge. Two brain regions, the hippocampus and ventromedial nucleus of the hypothalamus (VMH), had significantly different Fos expression profiles following seizures. Fos expression was highly robust in B6 hippocampus following one seizure and remained elevated following multiple seizures. Conversely, there was an absence of Fos (and phospho-Erk) expression in D2 hippocampus following one generalized seizure that increased with multiple seizures. This lack of Fos expression occurred despite intracranial electroencephalographic recordings indicating that the D2 hippocampus propagated ictal discharge during the first flurothyl seizure suggesting a dissociation of seizure discharge from Fos and phospho-Erk expression. Global transcriptional analysis confirmed a dysregulation of the c-fos pathway in D2 mice following 1 seizure. Moreover, global analysis of RNA expression differences between B6 and D2 hippocampus revealed a unique pattern of transcripts that were co-regulated with Fos in D2 hippocampus following 1 seizure. These expression differences could, in part, account for D2's seizure susceptibility phenotype. Following 8 seizures, a 28 day rest period, and a final flurothyl rechallenge, ∼85% of B6 mice develop a more complex seizure phenotype consisting of a clonic-forebrain seizure that uninterruptedly progresses into a brainstem seizure. This seizure phenotype

  19. Analysis of the energy efficiency of the transport system in Algeria; Analyse de l'efficacite energetique du systeme de transport en Algerie

    Energy Technology Data Exchange (ETDEWEB)

    Hamdani, Sid Ahmed


    The objective of this communication is analyze the energy efficiency of the transport system in Algeria and to show the areas of possible rationalization in this sector. Our approach is to analyze the existing configuration of the sector and its impact on energy consumption, by developing a sectional model Bottom Up, where the transport park has been modified by the means used. We have shown that the potential to improve the transport system energy efficiency is important and have recommended some options aimed at the sector organisation and aimed at increasing the relative part of transport systems to make it more energy efficient. [French] L'objectif de cette communication est d'analyser l'efficacite energetique du systeme de transport algerien et de montrer les gisements de rationalisation possibles dans ce secteur. Notre approche consiste a analyser la configuration existante du secteur et son impact sur la consommation d'energie, en elaborant un modele sectoriel Bottom Up, ou le parc de transport a ete desagrege par moyen utilise. Nous avons montre que le potentiel d'amelioration de la performance energetique du systeme de transport est important et avons recommande quelques options ciblant l'organisation du secteur et visant a augmenter la part relative de moyens de transport plus efficace energetiquement.

  20. Power system reliability memento; Memento de la surete du systeme electrique

    Energy Technology Data Exchange (ETDEWEB)



    The reliability memento of the French power system (national power transmission grid) is an educational document which purpose is to point out the role of each one as regards power system operating reliability. This memento was first published in 1999. Extensive changes have taken place since then. The new 2002 edition shows that system operating reliability is as an important subject as ever: 1 - foreword; 2 - system reliability: the basics; 3 - equipment measures taken in order to guarantee the reliability of the system; 4 - organisational and human measures taken to guarantee the reliability of the system; appendix 1 - system operation: basic concepts; appendix 2 - guiding principles governing the reliability of the power system; appendix 3 - international associations of transmission system operators; appendix 4 - description of major incidents.

  1. Evolution of the radiation protection system; L'evolution du systeme de protection radiologique

    Energy Technology Data Exchange (ETDEWEB)

    Clarke, R.H. [International Commission on Radiological Protection, Stockholm (Sweden); Schieber, C.; Cordoliani, Y.S. [Societe Francaise de Radioprotection, 92 - Fontenay aux Roses (France); Brechignac, F. [CEA Cadarache, Institut de Radioprotection et de Surete Nucleaire, Dept. de Protection de l' Environnement, 13 - Saint Paul Lez Durance (France)


    The evolution of the system of radiological protection: justification for new ICRP recommendations, thoughts of the SFRP work group about the evolution of the system of radiation protection proposed by the ICRP, protection of environment against ionizing radiations seen by the ICRP are the three parts of this chapter. (N.C.)

  2. Platerm: la banque de terminologie du systeme Platon (PLATERM: The Terminology Bank of the PLATO System). (United States)

    Schwab, Wallace; St-Denis, Richard


    Describes the elements and functioning of the terminology bank of the PLATO (Programmed Loqic for Automated Teaching Operation) system. Discusses contemporary terminology and lexicography notions on which the bank is based and outlines the tasks performed through PLATERM. (MES)

  3. Implementation of FCI heating system to the control system of Tore-Supra; Integration du systeme de chauffage FCI au sein du reseau de controle commande du Tokamak Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Wisniewski, S


    This report presents the implementation of the ion cyclotron resonance heating system (FCI) to the instrumentation and control system of the Tore-Supra tokamak. The new plasma heating system involves 3 antennas delivering 12 MW that are required to maintain fusion reactions. This paper is divided into 8 chapters: 1) thermonuclear fusion and Tore-Supra tokamak; 2) hardware system around Tore-Supra, in this chapter the control system and the data acquisition and processing systems are presented; 3) functional analysis, this analysis defines the different needs concerning timing and pilot-controlling, a preliminary proposition of hardware equipment is made; 4) operating modes of FCI; 5) communication within the control system network; 6) communication with the supervisory system of the power stations; 7) management of data exchange with SMX generators; and 8) control of the rate of stationary waves during the injection of power into the plasma.

  4. Next generation of energy production systems; Lancement pour les systemes du futur

    Energy Technology Data Exchange (ETDEWEB)

    Rouault, J.; Garnier, J.C. [CEA Saclay Dir. de l' Energie Nucleaire DEN, 91 - Gif sur Yvette (France); Carre, F. [CEA Saclay, Dir. du Developpement et de l' Innovation Nucleares - DDIN, 91 - Gif Sur Yvette (France)] [and others


    This document gathers the slides that have been presented at the Gedepeon conference. Gedepeon is a research group involving scientists from Cea (French atomic energy commission), CNRS (national center of scientific research), EDF (electricity of France) and Framatome that is devoted to the study of new energy sources and particularly to the study of the future generations of nuclear systems. The contributions have been classed into 9 topics: 1) gas cooled reactors, 2) molten salt reactors (MSBR), 3) the recycling of plutonium and americium, 4) reprocessing of molten salt reactor fuels, 5) behavior of graphite under radiation, 6) metallic materials for molten salt reactors, 7) refractory fuels of gas cooled reactors, 8) the nuclear cycle for the next generations of nuclear systems, and 9) organization of research programs on the new energy sources.

  5. Processing of next generation weather radar-multisensor precipitation estimates and quantitative precipitation forecast data for the DuPage County streamflow simulation system (United States)

    Bera, Maitreyee; Ortel, Terry W.


    The U.S. Geological Survey, in cooperation with DuPage County Stormwater Management Department, is testing a near real-time streamflow simulation system that assists in the management and operation of reservoirs and other flood-control structures in the Salt Creek and West Branch DuPage River drainage basins in DuPage County, Illinois. As part of this effort, the U.S. Geological Survey maintains a database of hourly meteorological and hydrologic data for use in this near real-time streamflow simulation system. Among these data are next generation weather radar-multisensor precipitation estimates and quantitative precipitation forecast data, which are retrieved from the North Central River Forecasting Center of the National Weather Service. The DuPage County streamflow simulation system uses these quantitative precipitation forecast data to create streamflow predictions for the two simulated drainage basins. This report discusses in detail how these data are processed for inclusion in the Watershed Data Management files used in the streamflow simulation system for the Salt Creek and West Branch DuPage River drainage basins.

  6. Cycle for innovative nuclear Gen 4. systems=; Cycle des systemes du futur

    Energy Technology Data Exchange (ETDEWEB)



    In the framework of the development of nuclear systems of the 4. generation, the preliminary and schematic reprocessing goals are a cleaning of fission products without a priori separation of the different actinides. The objective of the workshop is to exchange information about the potential efficiency of innovative fuel processing treatments in order to evaluate the impact of impurities on the design of the fuel during its re-fabrication and re-introduction inside the reactor, and on the materials and systems. This document gathers the slides of the 18 presentations given at this workshop: 1 - from the PWR fuel to the closed cycle fast spectrum concepts of generation 4 systems (P. Anzieu, F. Carre, Ph. Brossard, M. Delpech); 2 - the double strata scenarios: objectives and characteristics (S. David and F. Varaine); 3 - why a molten salts thorium file (D. Heuer); 4 - the common 'molten salts' research program of the CNRS (D. Heuer, S. Sanchez); 5 - the hydro-metallurgical reprocessing, the knowledge gained and the statuses of the 5. PCRD, synthesis of the OECD works (C. Madic); 6 - pyro-chemistry: Pyropep status (H. Boussier); 7 - technological bolts identified during the Most project of the 5. PCRD (C. Renault, Ch. Le Brun, M. Delpech and C. Garzenne); 8 - the molten salt reactor concept and its reprocessing options, expected efficiencies (L. Mathieu); 9 - methodology of evaluation of pyro-chemical fuel reprocessing schemes (H. Boussier); 10 - molten salt reactor, design-aided tools for the reactor and the reprocessing plant (O. Gastaldi, E. Walle, O. Koberl, D. Lecarpentier); 11 - status of CEA's prospective studies for the front-end of the fuel reprocessing process/dry ways (S. Bourg); 12 - results of activity coefficient measurements in liquid metals (J. Finne, E. Walle, G. Picard, S. Sanchez and O. Conocar); 13 - potentialities of electrolytic separation and liquid-liquid extraction processes (molten salts/molten metal) for the multi



    Madinier, Blandine


    International audience; Le suivi des performances passe par une analyse de la valeur perçue par le client et des coûts générés. L'analyse par processus et par activités peut constituer un cadre méthodologique intéressant pour construire un outil d'instrumentation de l'arbitrage coût - valeur. Cette problématique est étudiée à travers l'étude empirique du programme de fidélisation d'une compagnie aérienne.

  8. Charging system functioning of the Vivitron in voltage testings. New developments; Fonctionnement du systeme de charge du Vivitron lors des essais de tension. Nouveaux developpements

    Energy Technology Data Exchange (ETDEWEB)

    Helleboid, J.M.; Brandt, C.; Balzers, P.; Delhomme, C.; Gaudiot, G.; Haberer, A.; Koenig, R.; Krieg, C.; Misslin, F.; Neufert, O.; Osswald, F.; Riehl, F.; Schaeffer, E.; Schlewer, B.; Schuler, J.; Sontag, G.; Toussaint, J.P.; Vogler, H.


    The behaviour of the charging system for the initial commissioning of the Vivitron generator was quite satisfying apart from the excessive parasitic currents at the rollers. That led us to study and test many solutions up to the point that a humidity level higher than thought was discovered as the most probable cause of the problem.

  9. Increasing the reliability and capacity of Ukrainian gas transit system; Augmentation de la capacite et de la fiabilite du systeme Ukrainien de transport du gaz

    Energy Technology Data Exchange (ETDEWEB)

    Rozgonyk, V.; Osinchuk, Z. [Naftogaz of Ukraine (Ukraine)


    Ukrainian gas transmission system plays a key role in the transit deliveries of Russian natural gas to European countries. Therefore special attention is paid to reliability and efficiency of the existing gas pipelines. The in-line inspections of pipelines as well as update methods of corrosion pipeline protection control and developed vibration diagnostic system for gas compressor units are used on a large scale. The program for gas transmission pipeline reconstruction and upgrading of 50 compressor stations of main lines is being under realization. Modernization of compressor stations will allow to increase turbine drivers' efficiency up to 34-36 per cent to save about 1-10{sup 9} cubic meters of fuel gas annually. The reliability of gas transit deliveries is supported by powerful underground gas storage facilities, situated mostly in the Western Ukraine. The expansion of gas pipe lines system is carried out that will increase transit capacity of Ukrainian gas trunk lines up to 160 -10{sup 9} cubic meters a year. (authors)

  10. Quality control and risk management: specific characteristics of the French health care system;Fonction qualite et gestion du risque: reflexions sur les specificites du systeme francais

    Energy Technology Data Exchange (ETDEWEB)

    Franck, D. [Syndicat National des Radiotherapeutes Oncologues (SNRO), 75 - Paris (France); Bureau de la Societe Francaise de Radiotherapie Oncologique (SFRO), 75 - Paris (France); Couderc, B. [Union Nationale Hospitaliere Privee de Cancerologie (UNHPC), 75 - Paris (France); Institut National du Cancer (INCa), 75 - Paris (France); Ligue Contre le Cancer, 75 - Paris (France); Parmentier, G. [UNHPC, 75 - Paris (France); College de l' Accreditation, Haut Conseil de Sante Publique, 75 - Paris (France)


    The techniques used in the field of radiotherapy are the same everywhere, but the organisation and scope of activities vary depending on the country, and sometimes depending on the specific region. The International Radiotherapy Conference held in Paris and devoted to the radiation protection of radiotherapy patients provides us with an opportunity to discuss some of the specific characteristics of the French health care system. These past years, the context of workforce shortage associated with strong government initiatives, the implementation of several successive National Cancer Plans, the transposition of European Directives into French law (EURATOM 96/29 and 97/43) and the accident in Epinal have had a rapidly restructuring effect on the French radiotherapy sector. (authors)

  11. Spéciation du cadmium, du chrome, du cuivre et du plomb dans les ...

    African Journals Online (AJOL)

    L'exploitation du minerai de phosphate au sud-est du Togo engendre des déchets miniers qui sont déversés dans la mer sans traitement. Cette étude a pour but l'estimation de la biodisponibilité du cadmium, du chrome, du cuivre et du plomb par leur spéciation. La caractérisation physicochimique des sédiments des ...

  12. Developpement d'un catalyseur nickel-alumine efficace pour le reformage de diesel a la vapeur d'eau et etude du systeme reactionnel (United States)

    Fauteux-Lefebvre, Clemence

    utilisation n'a montre aucun carbone significatif sur la surface.- En comparaison, un catalyseur de nickel metallique sur support d'Al2O3 et YSZ a ete utilise dans des conditions similaires. Il y a eu desactivation du catalyseur et obstruction du reacteur par du carbone apres trois heures d'operation. L'analyse de ce catalyseur a permis de verifier qu'il etait recouvert de carbone en filament. L'analyse du systeme reactionnel a montre que la reaction est controlee par la reaction de. surface et non par le transfert de masse. Par ailleurs, les analyses des catalyseurs de spinelle ont demontre qu'il n'y avait pas de modification de sa forme chimique ni de reduction du spinelle en nickel metallique apres l'utilisation. Mots cles : Reformage a la vapeur, diesel, hydrocarbure liquide, catalyseur, spinelle nickel-alumine, equilibre thermodynamique

  13. Cognitive decline is associated with systemic oxidative stress: the EVA study. Etude du Vieillissement Artériel. (United States)

    Berr, C; Balansard, B; Arnaud, J; Roussel, A M; Alpérovitch, A


    To determine whether systemic oxidative stress status is associated with cognitive decline. A longitudinal population-based study. A cohort study of older subjects in Nantes, France. A total of 1166 high cognitive functioning subjects aged 60 to 70 in the Etude du Vieillissement Arteriel (EVA) cohort with a 4 year follow-up. Subjects completed a baseline interview and a global cognitive test (Mini-Mental Status Examination (MMSE)). Blood samples were obtained at baseline to determine plasma levels of selenium, carotenoids, thiobarbituric acid reactant substances (TBARS), an indicator of lipoperoxidation, and red blood cell vitamin E. Risk of cognitive decline, defined as a loss of 3 points in MMSE score between baseline and the 4 year follow-up, was assessed by oxidative stress level. Subjects with the highest levels of TBARS show an increased risk of cognitive decline (adjusted odds ratio (OR) = 2.25; confidence interval (CI) 95% = 1.26-4.02). This result is reinforced in the lower antioxidant status subgroup. Subjects with low levels of selenium have an increased risk of cognitive decline (OR = 1.58; CI 95% = 1.08-2.31) after adjustment for various confounding factors. These results suggest that increased levels of oxidative stress and/or antioxidant deficiencies may pose risk factors for cognitive decline. The direct implication of oxidative stress in vascular and neurodegenerative mechanisms that lead to cognitive impairment should be further explored.

  14. Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial

    Energy Technology Data Exchange (ETDEWEB)

    Chivot, J.J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    characteristic of its state of activity. The phagocytic index K depends also on the diameter of the colloidal particles injected and can be used as biological test for a colloid: this is the percentage of injected colloid which is retained by the S.R.E. per unit time. This index depends on anaesthetics, on the state of inflammation of the organism and on chemical mediators such as histamine. (author) [French] Un rappel historique met en lumiere le systeme reticulo-endothelial S.R.E. et tente d'en preciser l'identite et le fonctionnememt. Les methodes de son exploration et les colloides utilises sont envisages. L'auteur est amene a fabriquer un colloide d'un type particulier: un complexe albuminique radioiode le 'C.A. {sup 131}I' dont il decrit les etapes de la preparation et des essais tant physiques que biologiques. Une albumine humaine, presentant en solution une densite optique definie est portee a la chaleur jusqu'a modification de la densite optique, temoin d'une agregation des molecules proteiques. La proteine denaturee est iodee par {sup 131}I. Des controles d'electrophorese, d'ultracentrifugation et d'autoradiographie sont effectues. Cette preparation d'origine biologique atoxique et metabolisable sera comparee a l'or colloidal mieux defini qui servira de reference. Le C.A.{sup 131}I est injecte a la souris. Des mesures de radioactivite, des autoradiographies de coupe de l'animal entier, des detections de l'anthropogammametre mettent en evidence une concentration importante au niveau du S.R.E. du foie. Mais ces resultats statiques ont une portee limitee en regard de l'exploitation d'une etude cinetique in vivo du phenomene. L'auteur enregistre les variations de la radioactivite du sang derive de la carotide dans un scintillateur a puits. Il obtient directement une courbe de radioactivite sanguine d'allure exponentielle decroissante dont il definit la formule mathematique. La

  15. L’agriculture en Arabie du Sud avant l’Islam South Arabian Pre-Islamic Agriculture: Piecing Together Ancient Landscapes & Agricultural Systems

    Directory of Open Access Journals (Sweden)

    Julien Charbonnier


    Full Text Available Le présent article vise à reconstituer les différents systèmes de culture d’Arabie du sud antique. Pour ce faire, les études archéobotaniques sont mises en perspective avec les recherches sur les paysages et les techniques de culture anciennes. Il apparaît ainsi, qu’entre l’âge du Bronze et la période sudarabique, le Yémen n’a cessé de s’enrichir de l’arrivée de nouvelles plantes, en provenance de Proche-Orient, de l’Afrique et du Monde indien. Leurs capacités propres et les nouvelles combinaisons dont elles offraient l’opportunité ont permis à l’homme de diversifier les systèmes de culture afin de tirer au maximum parti des terres et d’être plus flexible face aux altérations du climat et de l’environnement.This paper aims at recognizing and understanding the various agro-systems of the ancient South Arabia. Archaeobotanical data are confronted with studies concerning pre-Islamic landscapes and agricultural techniques. It appears that, during the Bronze Age and the South Arabian period, new cultivars, coming from the Near-East, Africa and India, have regularly reached the region. Because of their own specificities and of the new associations they permitted, these plants have allowed Man to enrich agro-systems and to be more flexible in order to cope with climatic and environmental changes.

  16. Upgrade of GANIL control system cluster; Evolution du cluster de controle

    Energy Technology Data Exchange (ETDEWEB)

    David, L. [Grand Accelerateur National d`Ions Lourdes (GANIL), 14 - Caen (France)


    The GANIL control system was based on VAX 32 bit computers connected on a coaxial ethernet network. Since last year we are using an Alpha server with 64 bit Alpha workstations. At the same time the network is moving to twisted pair linked to a HUB concentrator, which is itself connected to the main FDDI ring. Solutions to solve the following problems are presented: the generalization of control graphical interfaces; extensive usage of the data base; the compilation of the more complex units; object programming; adaption to the new materials technologies; migration towards another operating system. Also described is the modification in the network imposed by the supplementary control of SPIRAL 2 figs.

  17. Development du systeme racinaire chez le palmier a huile selon l ...

    African Journals Online (AJOL)

    Rootsystem architecture is important in drought resistance, because of its role in water and nutrient mobilizations. The variability of root system architecture of 3 oil palm crosses B, C and E was studied. The 3 crosses were different for their sensitivity to drought. The plants were potted into plastic PVC tubes (95 cm in depth x ...

  18. Modernisation du syste`me d'enseignement et de recherche a` l ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Ayant e´te´ pendant longtemps un pays caracte´rise´ par sa stabilite´ politique et son essor e´conomique, la Co^te disposait d'un des syste`mes d'enseignement et de recherche universitaires les plus performants en Afrique de l'Ouest. Depuis le de´but des anne´es 2000, le pays e´tait entre´ dans une pe´riode de crise ...

  19. formes histologiques particulieres du cancer du nasopharynx

    African Journals Online (AJOL)

    19 déc. 2007 ... paroi latérale droite du cavum et de la face postérieure du palais mou avec refoulement en bas et en dedans de l'amygdale droite sans adénopathies .... des difficultés liées à la localisation anatomique du naso- pharynx, et à la nature infiltrante et extensive de ce type de tumeur ce qui expose au risque de ...

  20. Resetting dynamic behaviour of pipework systems; Recalage du comportement dynamique des reseaux de tuyauteries

    Energy Technology Data Exchange (ETDEWEB)

    Gaudin, M. [Electricite de France (EDF), Direction des Etudes et Recherches, 92 - Clamart (France)


    Resetting models is applied to electricity generating plant pipework systems. A frequency approach to the problem is made in an original way thanks to the use of precise dynamic rigidity matrices. The method assumes two kinds of unknown: the usually processed mechanical characteristics (Young`s Modulus, density etc.) and new resetting parameters acting on the dynamic behaviour of unknown connections. As the latter have a very wide range of possible variation, they benefit from a change of variable which allows the assumptions formulated to be complied with. The minimized cost function is based on a error in load. The frequencies required for building it are automatically selected thanks to different tests on measurements. Minimization uses a sensitivity technique linked with a method of least standard squares. The method has been programmed in Fortran 90 within the CIRCUS code and tried out on various examples which were simulated and sound effects cases as well as an actual case. (author). 128 refs.

  1. Cri du chat syndrome (United States)

    ... this page: // Cri du chat syndrome To use the sharing features on this page, please enable JavaScript. Cri du chat syndrome is a group of symptoms that result from ...


    African Journals Online (AJOL)

    RECOMMANDATIONS SPECIFIQUES D'ENGRAIS: CALIBRATION ET VALIDATION DU MODULE PHOSPHORE DU MODELE NuMaSS. M. D. Doumbia, A. Sidibé, A. Bagayoko, M. A. Diarra, A. Bationo, R. A. Kablan, R. S. Yost, L. R. Hossner, F. M. Hons ...

  3. Market opening and information systems; L'ouverture du marche et les systemes d'informations

    Energy Technology Data Exchange (ETDEWEB)

    Dardevet, G. [Gaz de Bordeaux, 33075 Bordeaux Cedex (France); Dardoise, P. [Gaz de France Reseau Distribution (France); Dreyer, P. [CRE, 2 rue du Quatre-Septembre- 75084 Paris Cedex 02 (France); Flahaut, P. [Altergaz, 24, rue Jacques Ibert - 92300 Levallois-Perret (France)


    The opening of energy markets to competition requires relevant information systems accessible to all end-users in concern without any discrimination. So, how existing information systems can be changed to become conformable with the requested requirements? Concerning the last step of the opening of energy markets which concerns several millions of clients, how this problem will be overcome considering the huge volume of processing needed? Who will decide or pay the forthcoming upgrades of the information system with respect to the evolution of actors' needs? Four participants were invited to this round table to answer these questions. Their answers are reported in this article. (J.S.)

  4. Replacement of the instrumentation and control system of Tore Supra; Remplacement du systeme de controle commande de Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Leveque, P.


    The control system of the Tore-Supra is a wide and complex system that cannot be interrupted while running without significant consequences on the operating of the machine. Replacing the current system cannot be achieved in a global way without immobilisation and high costs. Therefore partial changes have been decided on. This work presents the detailed analysis of the arrangements and the operating of the system that will be replaced: the pro`s and con`s that have appeared through experience are related. The possibilities that the new apparatus offers are also examined. A method of step by step replacements had to be set up in order to assess the means, funds, term of achievement, performance and quality of the overall project. (TEC). 15 refs., 29 figs.

  5. Nuclear systems of the future - generation 4. Proposals of strategic orientations for the nuclear systems of the future; Les systemes nucleaires du futur - generation 4. Propositions d'orientations strategiques pour les systemes nucleaires du futur

    Energy Technology Data Exchange (ETDEWEB)



    Several points, specific to France, must be taken into consideration for the long term strategic choice of future nuclear systems, in particular: taking the best profit of the progress potentialities of water reactors, optimizing the opportunities offered by the renewal of power plants in operation, integrating the consequences and the implementation of a strategy of optimized management of radioactive wastes, and looking for improvements that would make nuclear energy an active contributor to sustainable development. The prospective researches carried out by the CEA and its industrial partners have led to propose a R and D strategy with 3 complementary goals: search for innovations for water reactors, development of fast neutron reactors with closed fuel cycle (sodium fast reactor (SFR), gas fast reactor (GFR)), and development of key-technologies for nuclear hydrogen production (very high temperature reactor (VHTR)). The R and D effort concerns also the subcritical systems devoted to transmutation, the new cycle processes for a global management of actinides, and some other nuclear systems like the molten salt reactors (MSR) and the supercritical water reactors (SCWR). This paper presents the R and D strategy for each technology with its priorities, steps, financial means and collaborations. (J.S.)

  6. 2002 reliability report on the French power system; Bilan 2002 de la surete du systeme electrique francais

    Energy Technology Data Exchange (ETDEWEB)



    Ensuring the reliability of the power system is one of the main responsibilities entrusted to RTE, the French transmission system operator, by the Act of February 10, 2000. Reliability must be maintained, both now and in the future. This means that RTE is required to: ensure the normal operation of the power system, limit the number of incidents and avoid major incidents, limit the consequences of major incidents when they do occur. By publishing a yearly reliability report on the French power system, RTE is clearly showing its ambition to encourage long-term supervision of all aspects of system reliability development. By this approach, RTE's intention is to help develop a reliability culture, by all the actors concerned (both RTE and network users) more aware of their role in establishing reliability, and by promoting reliability issues and comparisons with international transmission system operator associations (UCTE , ETSO ). The annual reliability report is drawn up around the following themes: a broad overview of changes in the internal and external environment that have implications for reliability, developments in the various elements that make up the reliability referential during the course of the year, developments on measures aimed at promoting reliability, in terms of equipment, organisation and human resources, lessons learned from events that took place over the year, the presentation of reliability-related indicators, RTE's control loop, and the developments under way at present.

  7. Share-A-Ride rideshare matching system (SARRMS). Systeme de coordination du covoiturage Share-A-Ride (SCCSAR)

    Energy Technology Data Exchange (ETDEWEB)


    SARRMS (Share-A-Ride Rideshare Matching System) is an on-line, interactive system that produces up-to-date personalized matching information for rideshare applicants. SARRMS operates on microcomputers and is capable of handling a large number of requests with a high degree of accuracy and speed. The system is flexible enough to be used by employers, municipalities, and ridesharing agencies to provide rideshare matching service. It has been designed specifically for Canadian applications. The features of SARRMS are outlined, including the five software subsystems (Rideshare, Vanpool, Secondary File Maintenance, Report production in English or French, Purge, and Backup). Matching is done on basis of location, and a printout is obtained for the applicant which gives a list of possible pooling partners who have similar commuting characteristics, a list of vanpool operators and transit operators serving the applicant's zone, and a list of carpool parking lots based on commuting direction. A sample search is included.

  8. Prostate tracking feasibility by the BeamCath system; Faisabilite du tracking prostatique par le systeme BeamCath

    Energy Technology Data Exchange (ETDEWEB)

    Leysalle, A.; Nickers, P.; Lacornerie, T.; Lartigau, E. [Centre Oscar-Lambret, Dept. Universitaire de Radiotherapie-Oncologie, Unite Cyberknife nord-ouest, 59 - Lille (France)


    The objective is to describe the use of the 'Beamcath', urethra catheter containing opaque radio- tracers, as alternative to the implementation of intra prostate fiducial by perineal ducts for the realisation of a tracking in prostate stereotactic radiotherapy for a patient for whom the stop of anti coagulants is dissuaded. The 'Beamcath' appears as an interesting alternative with an excellent clinical tolerance for the patient. The validation of this repositioning system will be realised in a prospective manner. (N.C.)

  9. Fiche technique du spermogramme et du spermocytogramme ...

    African Journals Online (AJOL)

    Les causes de l'infertilité masculine peuvent être l'impuissance et/ ou l'altération du sperme. L'étude de l'altération du sperme est appréciée par le spermogramme et le spermocytogramme. Ces analyses doivent être réalisées par des laboratoires de biologie de la reproduction. Vu la rareté ou l'absence des laboratoires de ...

  10. Detection of Salmonella species in a variety of foods by the DuPont Bax system real-time PCR assay for Salmonella: first action 2013.02. (United States)

    Wallace, F Morgan; Andaloro, Bridget; Fallon, Dawn; Corrigan, Nisha; Varkey, Stephen; DeMarco, Daniel; Farnum, Andrew; Tadler, Monica; Hoelzer, Steven; Weller, Julie; Davis, Eugene; Rohrbeck, Jeffrey; Tice, George; Bird, Patrick; Crowley, Erin; Flannery, Jonathan; Fisher, Kiel; Huffman, Travis; Boyle, Megan; Benzinger, M Joseph; Bedinghaus, Paige; Goetz, Katie; Judd, William; Agin, Jim; Goins, David


    A multilaboratory study was conducted to evaluate the ability of the DuPont BAX System Real-Time PCR Assay for Salmonella to detect the target species in a variety of foods and environmental surfaces. Internal validation studies were performed by DuPont Nutrition & Health on 24 different sample types to demonstrate the reliability of the test method among a wide variety of sample types. Two of these matrixes-pork and turkey frankfurters and pasteurized, not-from-concentrate orange juice without pulp-were each evaluated in 14 independent laboratories as part of the collaborative study to demonstrate repeatability and reproducibility of the internal laboratory results independent of the end user. Frankfurter samples were evaluated against the U.S. Department of Agriculture, Food Safety and Inspection Service reference method as a paired study, while orange juice samples were evaluated against the U.S. Food and Drug Administration reference method as an unpaired study, using a proprietary media for the test method. Samples tested in this study were artificially inoculated with a Salmonella strain at levels expected to produce low (0.2-2.0 CFU/test portion) or high (5 CFU/test portion) spike levels on the day of analysis. For each matrix, the collaborative study failed to show a statistically significant difference between the candidate method and the reference method using the probability of detection statistical model.

  11. Renouvellement des eaux du fjord du Saguenay (United States)

    Belzile, Melany

    Le fjord du Saguenay, localise dans la region subarctique de l'est du Canada, ala particularite d' etre connecte a un estuaire tres energetique plutot que directement a 1' ocean. L'embouchure du fjord est situee ala rencontre d'un chenal profond et d'un seuil de 20m de profondeur qui limite les echanges d'eau entre le fjord et l'estuaire du Saint-Laurent. Cependant, les grandes amplitudes de maree a son embouchure ont le potentiel d'entrainer des eaux denses du Saint-Laurent a l'interieur du fjord renouvelant ainsi les eaux des differents bassins. Dans le but d' a voir une meilleure comprehension de la dynamique et de la saisonnalite des renouvellements dans le bassin interne, deux mouillages y ont ete deployes pour recolter les premieres donnees de courant, sur toute la colonne d' eau et sur plusieurs mois, ainsi que des observations de temperature et de salinite a differentes profondeurs. L'un de ces mouillages n' a malheureusement pas pu etre analyse en detail dfi a la complexite de ses resultats et au manque de temps. Des profils ont ete recoltes le long de section transversales (transects), couvrant plusieurs saisons et plusieurs annees, ce qui a contribue a une meilleure comprehension de la distribution spatiale des masses d'eau dans le fjord. Les resultats montrent que ladynamique du fjord est plus complexe que ce qui avait ete precedemment presente dans la litterature. Un changement saisonnier abrupte dans la circulation du fjord a ete observe a la rni-fevrier. De plus, des renouvellements non-anticipes juste en-dessous de la thermohalocline (˜ 10 m de profondeur) ont ete observes entre la fin de 1' hiver et le milieu de 1' ete. En fonction des saisons, trois types de renouvellement peuvent etre observes : renouvellements profonds a l'automne et au debut de l'hiver suivis des renouvellements de sous-surface et enfin les renouvellements a des profondeurs intermediaires pendant l'ete. Le changement saisonnier abrupte observe au milieu de l'hiver ainsi que la

  12. Les Cahiers du CREAD

    African Journals Online (AJOL)


    information sur certaines d'entre elles, elles ont été exclues de facto du calcul. 2. La description du modèle MIMIC. Comme mentionné plus haut, le modèle MIMIC comprend deux ensembles d'équations: l'équation structurelle et l'équation de ...

  13. "Cirque du Freak." (United States)

    Rivett, Miriam


    Considers the marketing strategies that underpin the success of the "Cirque du Freak" series. Describes how "Cirque du Freak" is an account of events in the life of schoolboy Darren Shan. Notes that it is another reworking of the vampire narrative, a sub-genre of horror writing that has proved highly popular with both adult and…

  14. Withania somnifera (L.) Dunal root extract alleviates formalin-induced nociception in mice: involvement of the opioidergic system. (United States)

    Orrù, Alessandro; Casu, Maria Antonietta; Tambaro, Simone; Marchese, Giorgio; Casu, Gianluca; Ruiu, Stefania


    Withania somnifera (L.) Dunal extracts (WSEs) may possess therapeutic perspectives in the treatment of inflammation and pain. We aimed to evaluate the antinociceptive property of a WSE in the formalin test and to investigate the involvement of several neurotransmitter systems in this effect. The time spent licking the formalin-injected paw was recorded in CD1 mice after pretreatment with increasing doses of WSE. Also, c-Fos spinal cord expression and the effects of different compounds were investigated under these experimental conditions. Finally, the efficacy of WSE was analyzed following an injection of glutamate. WSE reduced the antinociceptive response during the tonic but not the acute phase of the formalin test and decreased formalin-induced c-Fos expression in spinal neurons. These effects were antagonized by the opioid antagonist naltrexone, whereas GABA, cannabinoid, δ-opioid, and nitric oxide compounds were ineffective. The administration of WSE also reduced nociception and c-Fos expression induced by glutamate injection. These results showed that WSE is effective in assays of chemical-induced nociception, indicating that this plant has potential valuable properties for the treatment of specific painful conditions. The antinocicetive effects of WSE in the formalin test appeared to be specifically mediated by the opioidergic system, although the involvement of the glutamatergic system cannot be excluded.

  15. Ecologie du phytoplancton du lac Kivu

    Directory of Open Access Journals (Sweden)

    Sarmento, H.


    Full Text Available Speciation within the African Coffee Pathogen. Cet article analyse s'il est avantageux d'utiliser le compost au lieu de l'engrais minéral pour produire la laitue dans la zone urbaine et péri-urbaine de Yaoundé. Les résultats de terrain montrent l'obtention de rendements et profits plus élevés lorsqu'on utilise le compost. Les résultats de la fonction de production Cobb-Douglas prouvent que l'utilisation du compost est statistiquement significative pour expliquer la variation de rendement de la laitue et que le compost est l'intrant le plus productif. D'autres résultats montrent que le compost fournit la matière organique utile au sol et que les besoins d'irrigation en eau de la culture sont réduits grâce à l'utilisation du compost. Par conséquent, malgré le fait que l'application du compost demande une main-d'oeuvre beaucoup plus élevée, son utilisation est généralement bénéfique pour les agriculteurs vivant aux alentours de Yaoundé. Les programmes de vulgarisation de cet intrant pour encourager son adoption devraient donc figurer parmi les points prioritaires dans la politique agricole du gouvernement camerounais.

  16. European multicentre evaluation of the Du Pont Dimension 380 under the auspices of the European Group for the Evaluation of Analytical Systems in Laboratory Medicine (EGE-Lab). (United States)

    Hänseler, E; Vonderschmitt, D; Haeckel, R; Römer, M; Collombel, C; Goudable, J; Pourcher, E


    The Clinical Chemistry Analyzer Dimension 380 manufactured by Du Pont de Nemours was tested in a multicentre evaluation according to the guide-lines of the European Committee for Clinical Laboratory Standards (ECCLS) and in part to the protocol of the Société Française de Biologie Clinique (SFBC). The instrument and the reagents were evaluated as a system, since both reagents and reagent cartridges are specifically designed for the instrument. Fourteen analytes including electrolytes, substrates and enzymes were tested. The evaluators summarized their experience as follows: 1. All parameters tested yield results comparable to established procedures. 2. Very good performance of the ion-selective-electrode unit. 3. The imprecision data of the system are, for most parameters, between 1 and 4% CV and thus equal to or better than those of the instruments compared. 4. No reagent or sample carry-over was detected after a minor modification of the instrument. 5. The linearity of Dimension test methods in general covers the range stated by the manufacturer. 6. Very good stability of the calibration curves (up to 2 months). 7. Good practicability of the whole system, including handling of reagents and a very user-friendly software.

  17. Les Cahiers du CREAD

    African Journals Online (AJOL)


    Cet article est un produit du projet PNR 27 portant sur entrepreneuriale féminine en. Algérie. *. Professeur, Directeur du ... financement, réseaux, styles de gestion et performance (Brush, 1992; Bruin, Brush & Welter, 2007; ... tions et ses besoins, sa perception et la prise de risque), qu'à l'envi- ronnement, ses spécificités, ses ...

  18. Prenatal diagnosis of cri du chat syndrome with encephalocele. (United States)

    Bakkum, Jamie N; Watson, William J; Johansen, Keith L; Brost, Brian C


    A 19-year-old primigravida was found to have an encephalocele on screening ultrasound study. Amniocentesis indicated cri du chat syndrome, 5p-. Although cri du chat syndrome has been noted in association with central nervous system malformations, encephalocele is a rare finding in this syndrome.

  19. Soldier Mobility: Innovations in Load Carriage System Design and Evaluation (la Mobilite du combattant: innovations dans la conception et l’evaluation des gilets d’intervention) (United States)


    et du confort du futur combattant de l’OTAN, il est nécessaire d’approfondir les connaissances des pays membres de l’Alliance concernant les facteurs...sweating and inappropriate foot care . Metatarsalgia, stress fractures and knee problems are related primarily to load mass and duration of marching...however, sudden increases in march intensity or distance will probably make blisters more likely, regardless of training regularity. Moist skin increases

  20. Population genetic structure and mating system in the hybrid zone between Pinus sibirica Du Tour and P. pumila (Pall. Regel at the Eastern Baikal Lake shore

    Directory of Open Access Journals (Sweden)

    E.A. Petrova


    Full Text Available Genetic structure of sympatric Pinus sibirica Du Tour and P. pumila (Pall. Regel populations and putative interspecific hybrids between them was analyzed in the Baikal Lake region (Barguzin Biosphere Natural Reserve, Davsha River basin by means of 31 allozyme loci controlling 18 enzyme systems. Several alleles at loci Adh-1, Fest-2, Lap-3, Pgi-1, Sod-3 and Skdh-1 were diagnostic for P. sibirica, while alleles typical for P. pumila were detected at loci Gdh, Got-3, Lap-3, Mdh-2, Mdh-4, Pepca, Pgi-1, Pgd-2, Pgd-3, Pgm-1 and Pgm-2. All hybrids were heterozygous for the diagnostic Skdh-2 locus. Classification into hybrids and parental species using PCA analysis of multilocus allozyme genotypes had good correspondence with diagnoses made by morphological and anatomical analyses. Approximately 27% of embryos in P. pumila seeds had P. sibirica paternal contribution, and 8% of haplotypes in effective pollen pool combined alleles typical for P. pumila and P. sibirica, and therefore were classified as pollinated by the hybrids. About 83% of embryos in seeds from the hybrids most likely originated from fertilization by P. sibirica pollen, 14% from P. pumila and 3% from hybrid trees. This result favours the view that hybrids make both male and female contributions to the reproductive output of the population and confirm the presence of backcrosses and F2 hybrids.

  1. Population genetic structure and mating system in the hybrid zone between Pinus sibirica Du Tour and P. pumila (Pall. Regel at the Eastern Baikal Lake shore

    Directory of Open Access Journals (Sweden)

    E.A. Petrova


    Full Text Available Genetic structure of sympatric Pinus sibirica Du Tour and P. pumila(Pall. Regel populations and putative interspecific hybrids between them was analyzed in the Baikal Lake region (Barguzin Biosphere Natural Reserve, Davsha River basin by means of 31 allozyme loci controlling 18 enzyme systems. Several alleles at loci Adh-1, Fest-2, Lap-3, Pgi-1, Sod-3 and Skdh-1 were diagnostic for P.sibirica, while alleles typical for P. pumila were detected at loci Gdh, Got-3, Lap-3,Mdh-2, Mdh-4, Pepca, Pgi-1, Pgd-2, Pgd-3, Pgm-1 and Pgm-2. All hybrids were heterozygous for the diagnostic Skdh-2 locus. Classification into hybrids and parental species using PCA analysis of multilocus allozyme genotypes had good correspondence with diagnoses made by morphological and anatomical analysis.Approximately 27% of embryos in P. pumila seeds had P. sibirica paternal contribution, and 8% of haplotypes in effective pollen pool combined alleles typical for P. pumila and P. sibirica, and therefore were classified as pollinated by the hybrids. About 83% of embryos in seeds from the hybrids most likely originated from fertilization by P. sibirica pollen, 14% from P. pumila and 3% from hybrid trees.This result favours the view that hybrids make both male and female contributions to the reproductive output of the population and confirm the presence of backcrosses and F2 hybrids.

  2. Dissecting the Underlying Pharmaceutical Mechanism of Chinese Traditional Medicine Yun-Pi-Yi-Shen-Tong-Du-Tang Acting on Ankylosing Spondylitis through Systems Biology Approaches. (United States)

    Xie, Duoli; Huang, Lin; Zhao, Guanghui; Yu, Yiran; Gao, Jiawei; Li, Haichang; Wen, Chengping


    Traditional Chinese Medicine (TCM) has been served as complementary medicine for Ankylosing Spondylitis (AS) treatment for a long time. Yun-Pi-Yi-Shen-Tong-Du-Tang (Y-Y-T) is a novel empirical formula designed by Prof. Chengping Wen. In this study, a retrospective investigation supported efficacy of Y-Y-T and then we deciphered the underlying molecular mechanism of the efficacy. Herbal ingredients and targeting proteins were collected from TCMID. PPI networks were constructed to further infer the relationship among Y-Y-T, drugs used for treating AS, differentially expressed genes of AS patients and AS disease proteins. Finally, it was suggested that TLR signaling pathway and T cell receptor signaling pathway may involve in the biological processes of AS progression and contribute to the curative effect and proteins such as JAK2, STAT3, HSP90AA1, TNF and PTEN were the key targets. Our systemic investigation to infer therapeutic mechanism of Y-Y-T for AS treatment provides a new insight in understanding TCM pharmacology.

  3. Petit guide du ciel

    CERN Document Server

    Pellequer, Bernard


    Pour partir à la découverte du ciel nocturne, un petit guide simple, sûr et complet. Le tour du ciel en 32 cartes, pour apprendre à reconnaître les étoiles, mais d'abord à les observer - avec le meilleur des instruments : l'oeil. Des conseils pratiques d'observation, des cartes imprimées avec une encre fluorescente, les constellations visibles des deux hémisphères, Nord et Sud - de quoi mettre le ciel dans sa poche.

  4. Study of the optical monitoring system of the scintillating crystal involved in the electromagnetic calorimeter of CMS experiment; Etude du systeme de suivi optique des cristaux scintillants du calorimetre electromagnetique de l`experience CMS

    Energy Technology Data Exchange (ETDEWEB)

    Geleoc, M


    The prospect of the experimental discovery of the Higgs boson is one of the motivations to build the large hadron collider (LHC). Proton beams will collide and the emitted particles will be detected by ATLAS and CMS equipment. In each detector the electromagnetic calorimeter will allow the characterisation of the 2 photons coming from one of the disintegration channels of the Higgs boson. CMS collaboration has chosen an homogeneous calorimeter fitted with PbWO{sub 4} crystals. Each crystal with its photodetector and its electronic device forms one detection channel. The resolution of the detection channels should not deteriorate all along the operating time. The optical monitoring system of the crystals logs then controls the response of each detection channel in order to allow an accurate calibration of the calorimeter. The optical properties, the resistance to irradiation of PbWO{sub 4} crystals and the modelling of light collection are investigated in this work. The description of the different components of the optical monitoring system highlights the technical difficulties we had to challenge. An experimental testing bench has been set up to study the coupling between the scintillation signal and the signal that feeds the monitoring system, this coupling has been studied under irradiation in the conditions of CMS operating. (A.C.) 94 refs.

  5. Les 2x12h : une solution au conflit de temporalités du travail posté ? The 12-hour shifts system: A way to settle conflicting temporalities in shift work

    Directory of Open Access Journals (Sweden)

    Béatrice Barthe


    Full Text Available Les horaires de travail en 2x12h semblent faciliter l’articulation des temporalités mise à mal par le travail posté, articulation avec les dimensions temporelles du fonctionnement humain : les rythmicités biologiques, l’organisation de la vie socio-familiale et la fatigue notamment. Dans un contexte social et économique favorable à la diffusion des 2x12h, cet article propose une synthèse des effets de ces horaires de travail posté sur la santé, sur la vie personnelle des opérateurs concernés et sur le travail. Même si, comparés aux autres systèmes d’horaires, les 2x12h semblent avoir des conséquences moindres sur la santé et la vie personnelle, cette synthèse montre l’importance d’autres facteurs (caractéristiques des personnes, temps d’exposition aux horaires, propriétés du système d’horaires, par exemple sur ces effets et la nécessité d’intégrer l’analyse du contenu du travail et des exigences du travail dans l’analyse de ces effets. Avant de penser le 2x12h comme une solution au conflit de temporalités du travail posté, la réflexion d’aménagement des horaires de travail se doit d’intégrer une analyse systémique et détaillée du travail, prenant en compte le contenu du travail, les exigences des tâches et les stratégies d’accord des temporalités mises en place par les opérateurs concernés au niveau de leur activité de travail.Twelve-hour shifts appear to facilitate the flow of temporalities involved in shift work schedules and the different temporal dimensions of human functioning: particularly biological rhythms, private life, and fatigue. In a social and economic context that favors the increase of 12-h shifts, this paper sums up the effects of 12-h shifts on health, personal life, and work. 12-h shifts seem to have less impact on health and personal life, when compared with other shift-work systems. This synthesis shows the importance of other factors (characteristics of the

  6. Historique du Web

    CERN Multimedia

    TV8 Mont-Blanc


    Documentaire court qui retrace l'implication de différentes personalités à la création du Web. Entrevues avec Robert Cailliau, Chris Llewellyn-Smith, David Williams, Tim Berners-Lee, Mike Sendall, Brian Carpenter.

  7. du Chott Marouane

    African Journals Online (AJOL)

    Du point de vue biochimique, la déficience en acide éicosapentaénoique. (EPA) dans les échantillons n'est pas compromettante puisqu'une modification ... Abatzopoulos, J.A. Beardmore, J.S. Cleg,. P. Sorgeloos (Eds). pp. 171-224. Kluwer. Academic Publishers. Dordrecht, The. Netherlands, 2002. [2] C. Barigozzi, Genus ...

  8. La foret du Banco

    NARCIS (Netherlands)

    Koning, de J.


    Deze publicatie over het Nationaal Park 'La Forêt du Banco' nabij Abidjan, Ivoorkust, verschijnt als resultaat van vier jaar veldwerk (1972-1976) in dat land.

    Het Centre Néerlandais, onderzoeks- en stageverblijf van de Landbouwhogeschool, was basis en de faciliteiten verleend door

  9. La sauvegarde du patrimoine instrumental



    LA SAUVEGARDE DU PATRIMOINE INSTRUMENTAL Conférence-débat du 6 juin 2001, Orsay Ouverture par Raymond Duval, délégué régional du CNRS Ile-de-France Sud et introduction de la journée par André Kaspi, président du Comité pour l’histoire du CNRS. Que conserver et comment ? A - L’instrumentation scientifique, un élément essentiel du patrimoine, José Teixeira, Laboratoire Léon Brillouin, CNRS-CEA B - La disparition des matériels et du savoir-faire en neurosciences, Jacques Stinnakre, Laboratoire d...

  10. Il sistema penale through the looking glass / Le système pénal de l'autre côté du miroir / Criminal system through the looking glass

    Directory of Open Access Journals (Sweden)

    Pasculli Maria Antonella


    Full Text Available L’articolo analizza le più interessanti teorie in tema di filosofia del linguaggio per leggere diversamente il processo penale. Il sistema accusatorio può, infatti, essere interpretato attraverso tali tecniche di lettura alternative ai consueti metodi esegetici (giurisprudenza e dottrina, rimandando agli usi perfomativi del linguaggio studiati da J. L. Austin nella sua elaborazione formale degli atti locutori, illocutori, perlocutori. Lo studio verte per profili argomentativi sulle funzioni dei giudici nella fase preliminare delle indagini, sul valore del termine prova e sulla capacità decidendi del giudicante in una proiezione “metalinguistica” del processo di parti.L’article analyse les théories les plus intéressantes de la philosophie du langage afin de lire différemment le procès pénal. En effet, le système accusatoire peut être interprété par ces techniques de lecture qui sont alternatives aux méthodes exégétiques les plus courantes (jurisprudence et doctrine. L’utilisation de la philosophie du langage remonte aux théories de J.L. Austin sur les actes locutoires, illocutoires et perlocutoires. Le sujet principal de cette étude a trait aux fonctions des juges dans la phase de l’enquête préliminaire, la valeur du mot « preuve » et les motifs et le dispositif de la décision du juge en considération de la projection métalinguistique du procès accusatoire.The article will discuss the primary linguistic theories in order to examine criminal proceedings. The accusatorial system, in fact, could be interpreted through alternative analysis to usual exegetical methods (i.e. case law and legal doctrine. The original use of philosophy of linguistics dates back to J. L. Austin's development of performative utterances and his theory of locutionary, illocutionary, and perlocutionary acts. Judgements, evidences, and decisions are taken to include such acts as promising, ordering, greeting, warning and inviting. This

  11. Le ministre du Commerce international du Canada rencontre des ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    17 juil. 2017 ... La promotion de l'entrepreneuriat, la façon dont le commerce peut profiter aux femmes et à leur famille, et la création d'emplois pour les plus vulnérables étaient au coeur de la discussion en table ronde du ministre du Commerce international du Canada, l'honorable François-Philippe Champagne, et des ...

  12. Aux origines du monde

    CERN Document Server


    "C'est l'histoire d'une aventure humaine, scientifique, international qui a vu le jour il y a cinquante ans, aux confins de la Suisse et du département de l'Ain. Le plus grand laboratoire de physique des particules du monde, le Cern, a été fondé en 1954. Les festivités organisées à l occasion de cet anniversaire connaîtront leur point d'orgue le 16 octobre prochain, avec portes-ouvertes, accueil de personallités et inauguration d'un monumnet spécifique, le Globe de l'innovation" (2 pages)


    CERN Multimedia



    Les répétitions du chœur du CERN reprendront le mercredi 15 septembre à 20.00 heures à l’amphithéâtre principal – bâtiment 500. Au programme la préparation de notre concert de Noël avec la Missa Brevis, KV115, de Léopold Mozart et de la musique de Noël d’Europe. Les personnes qui aiment chanter, notamment des sopranes et des ténors, sont les bienvenues. Pour tout contact s’adresser à : Baudouin Bleus - (tél.CERN 767 82 44) -( ou Martin Gatehouse ( ou Jean-Paul Diss (  

  14. Le partage du paysage


    Sgard, Anne


    Ce mémoire de HDR présente un parcours de recherche en géographie sur le paysage, mené comme un essai sur cet objet plutôt qu'un itinéraire personnel. Partant d'une réflexion sur les évolutions récentes du concept de paysage, il défend une conception se référant à la médiance, qui aborde le paysage comme une modalité de la relation individuelle et collective au territoire, fondée sur la reconnaissance du sensible et de l'attachement à des paysages ordinaires ou remarquables. Un ensemble de dé...

  15. Bulletin du CRDI #123

    International Development Research Centre (IDRC) Digital Library (Canada)

    Les travaux de recherche financés par le CRDI promeuvent l'égalité entre les sexes et contribuent au bout du compte aux objectifs de développement durable de ... Cinq consultations régionales soutenues par le CRDI ont permis d'explorer le rôle des think tanks, notamment en ce qui a trait à l'ODD no. 3 lié à la santé.

  16. Les Cahiers du CREAD

    African Journals Online (AJOL)


    Résumé : Les motivations du recours aux opérations de croissance externe sont multiples et spécifiques à chaque entreprise. Dans cet article, nous nous intéressons à la motivation due aux effets des synergies de coûts résultant de la réunion des chaînes de valeur business de la cible et de l'acquéreur dans une opération ...

  17. Les Cahiers du CREAD

    African Journals Online (AJOL)


    Résumé : C'est dans un climat de crise économique où les pouvoirs publics perdent la capacité de financement et de développement des transports en commun et devant la déficience financière des entreprises publiques qu'est intervenue la levée du monopole de l'Etat sur l'activité des transports collectifs et son ouverture ...

  18. Les Conidae du Surinam

    NARCIS (Netherlands)

    Mol, van J.-J.


    RÉSUMÉ Les prospections des navires océanographiques Snellius et Luymes au large des côtes du Surinam révèlent l'existence de sept espèces de Conidae. Parmi celles-ci une espèce, Conus guyanensis spec. nov., semble endémique à cette région. Six espèces (Conus centurio, C. austini, C. daucus, C.

  19. La mesure du danger

    CERN Document Server

    Manceron, Vanessa; Revet, Sandrine


    La mesure du danger permet d’explorer des dangers de nature aussi diverse que la délinquance, la pollution, l’écueil maritime, la maladie ou l’attaque sorcellaire, l’extinction d’espèces animales ou végétales, voire de la Planète tout entière. Au croisement de la sociologie, de l’anthropologie et de l’histoire, les différents articles analysent les pratiques concrètes de mesure pour tenter de comprendre ce qui se produit au cours de l’opération d’évaluation du danger sans préjuger de la nature de celui-ci. L’anthropologie a contribué à la réflexion sur l’infortune en s’intéressant aux temporalités de l’après : maladies, catastrophes, pandémies, etc. et en cherchant à rendre compte de l’expérience des victimes, de leur vie ordinaire bouleversée, de la recomposition du quotidien. Elle s’intéresse aussi aux autres types de mesures, les savoirs incorporés, qui reposent sur l’odorat, la vue ou le toucher et ceux qui ressortent d’une épistémologie « non ...

  20. Har du sikret din alderdom?

    DEFF Research Database (Denmark)

    Kiens, Bente; Aagaard, Peter Gjerndrup


    Daglig fysisk aktivitet kan betragtes som indbetaling op din personlige helbredspension. Jo mere du sætter ind på kontoen, og jo oftere du gør det, jo større er sandsynligheden for, at du lever længere og får et sundere helbred. Og det er aldrig for sent at starte med indbetalingerne....

  1. Une mesure différente du bien-être : l'indice du bonheur national ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Une mesure différente du bien-être : l'indice du bonheur national brut du Bhoutan 2010. La demande de mesures novatrices, mais rigoureuses, du bien-être national allant au-delà du produit national brut ne cesse de croître. ... Réglementation du marché du travail en Chine : politiques en matière de salaire minimum.

  2. discours du Directeur General

    CERN Multimedia

    CERN. Geneva


    L'année 2007 est une année très spéciale pour le CERN. Je vous propose de nous retrouver pour faire le point sur les activités en cours. Rendez-vous le mercredi 27 juin à 15h00 dans l'amphithéâtre principal. La transmission simultanée sera assurée dans les amphithéâtres IT, AT, AB Prevessin et dans la Salle du Conseil. Robert Aymar

  3. Bulletin du CRDI #126

    International Development Research Centre (IDRC) Digital Library (Canada)

    il y a 6 jours ... Les barrières culturelles, couplées à un système de santé inefficace restreignant l'accès des femmes aux soins de santé, expliquent la précarité de la santé et des droits génésiques des Pakistanaises. Découvrez comment le centre Shirkat Gah, avec le soutien du CRDI, mise sur la sensibilisation pour ...

  4. L'Origine du Monde

    CERN Multimedia

    CNET & Ecole Polytechnique Paris; Vincent Ferreira


    Création, théologie, science, découverte, recherche, religion, condition humaine.Avec Maurice Jacob physicien, Hubert Curien Président du Conseil du CERN, Michael Doser physicien, Frère Emile Communauté de Taizé.

  5. Cri-du-chat syndrome

    Directory of Open Access Journals (Sweden)

    Eka Agustia Rini


    there is no cure for cri-du-chat syndrome. The most successful approach in the management of children with CDCS requires a multidisciplinary team approach. 4 The case presented below will remind us how to reveal, suspect and diagnose Cri-Du-Chat syndrome, a rare case in pediatric.

  6. Modeling of a tram power network: from the component to the system; Modelisation d'un reseau electrique de tramway: du composant au systeme

    Energy Technology Data Exchange (ETDEWEB)

    Morin, E.


    The study and the modeling of tram power networks require a system analysis. The use of several power-electronic converters in these networks significantly modifies both their topology and their function. Therefore, modeling methods developed in this thesis differ from traditional approaches, since a global analysis of a system should be linked with the study of its components.To satisfy the demands of the tram domain, this thesis develops modeling methods for transmission lines and transformers. Two levels of analysis are considered. Firstly, the tram power network is evaluated in relation to time. Secondly, an Iterative Harmonic Analysis (IHA) program is developed to compute the spectra of the system accurately. (author)



    Garambois, Nadège; Devienne, Sophie


    N° ISBN - 978-2-7380-1284-5; International audience; Since 1990, farmers of Poitou's Haut-Bocage (center West of France) have set up cattle grassland systems based upon André Pochon‘s Britain example and largely based on grazing of temporary grass-clover pastures. Adapted by farmers' groups to the specific pedoclimatic conditions of this region, these innovative systems create high value added by important cost savings, in complete contrast to the main agricultural development in the region, ...

  8. Nuclear systems of the future. Stakes, R and D strategy, and international cooperation; Les systemes nucleaires du futur. Enjeux, strategie de recherche et developpement, et cooperation internationale

    Energy Technology Data Exchange (ETDEWEB)



    As demonstrated by prospective studies, nuclear energy will represent a decisive contribution in the future energy mix. The long-term strategy of development of nuclear energy requires to foresee a new generation of nuclear systems, named generation 4. The goal of these new systems is to optimize the use of nuclear fuels, to minimize the generation of wastes and to enlarge the field of applications of nuclear energy to other applications like: hydrogen and synthetic fuels generation, heat generation for the industry etc. This document presents the French R and D strategy on nuclear systems of 4. generation that has been approved by the public authorities. This strategy follows three axes: a priority research on fast neutron systems with fuel recycle (sodium fast reactors (SFR) and gas fast reactors (GFR)), a research on key-technologies for the supply of very high temperature heat (very high temperature reactor (VHTR), fast and thermal neutron reactors, and water decomposition processes), and a continuation of researches on PWR reactors improvement. An integral recycling of all actinides in fast neutron reactors requires the development of new fuel reprocessing and fuel re-fabrication processes. A reference scenario for the progressive renewal of French nuclear facilities foresees the simultaneous development of fast neutron systems and the start-up of a new spent fuel reprocessing plant. France in involved in the development of the SFR, GFR and VHTR systems thanks to its participation to the Generation 4 international forum and to bilateral cooperation with other big nuclear partners like Russia and China. One of the main stakes of the French nuclear industry is to be able to invest in the R and D of future nuclear systems in order to valorize the experience gained so far in sodium FBR systems and in fuel cycle processes. (J.S.)

  9. Le paradoxe du linguiste

    Directory of Open Access Journals (Sweden)

    Primož Vitez


    Full Text Available Le linguiste, dans sa volonté explicite de s'imposer comme sujet scientifique et de décrire objectivement la langue comme un objet qui lui est extérieur, est paradoxal, parce que, du fait même qu'il est habité de sa langue, il se trouve nécessairement dans l'impossibilité de remplir la tâche qu'il se propose. Le paradoxe du linguiste consiste donc en cela que, si on se permet de paraphraser l'observation de Labov, « les faits de la langue s'étudient selon la vision de tel ou tel linguiste individuel, alors que le linguiste n'existe que par son expérience personnelle des faits linguistiques ». Cela signifie très simplement que le linguiste, en tant que descripteur d'une langue, n'est opératif qu'à l'intérieur de son objet d'analyse. La crédibilité de l'analyste dépend entièrement de la position éthique qu'il prendra vis-à-vis de sa recherche.

  10. Choeur du CERN : Concert

    CERN Multimedia

    CERN Choir


    Une œuvre à découvrir! La grande Missa pro defunctis de François-Joseph Gossec (1734-1829) est le chef-d’œuvre tôt venu (à vingt-cinq ans) d’un compositeur qui vivra encore 70 ans après sa création. Elle a connu la gloire, puis s’est fait un peu oublier. Pas du tout le monde cependant : des musicologues ont montré ce que le Requiem de Mozart lui devait ; et il suffit de l’avoir entendue pour comprendre pourquoi Berlioz (qui avait vingt-six ans à la mort de Gossec) en a été impressionné : les nombreux cuivres et bois répartis dans des endroits plus ou moins cachés de la salle de concert pour exprimer les frayeurs du Jugement dernier annoncent son Requiem – et celui de Verdi. Mais « plus encore que par...

  11. Les risques du travail

    CERN Document Server

    Thébaud-Mony, Annie


    Depuis les années 1990, les conditions de travail se sont peu à peu imposées dans le débat social. Néanmoins, la situation reste critique. Les risques traditionnels n'ont pas disparu : les manutentions lourdes, l'exposition professionnelle aux cancérogènes, au bruit ou aux vibrations demeurent répandues... De plus, certaines " améliorations " n'ont fait que déplacer et dissimuler les problèmes, telle l'externalisation des risques grâce à la sous-traitance. Dans le même temps, les transformations du travail et des modalités de gestion de la main-d'œuvre ont fragilisé les collectifs et accru l'isolement des salariés, conduisant à une montée visible de la souffrance psychique. Face à ces évolutions, il est plus que jamais nécessaire que tous les acteurs concernés, en particulier les salariés eux-mêmes et leurs représentants, s'approprient les connaissances indispensables pour améliorer la protection de la santé sur les lieux du travail. Tel est le but de ce livre, qui renouvelle int�...

  12. Les formes du fond

    Directory of Open Access Journals (Sweden)

    Michel Maffesoli


    Full Text Available Il n'est pas vrai que la nature a horreur du vide. Peut-être même s'y complait-elle. Le creux est aussi une modalité de l'être. Il est possible de s'y nicher, de s'y lover paresseusement et, ainsi, de se protéger contre l'angoisse du temps qui passe. Le creux des apparences est, à certains moments, une des formes d'expression de la vie sociale. Encore faut-il savoir le reconnaître. Certes, nous avons tous une existence personnelle, mais nous sommes, également, les représentants, parfois même les victimes, d'un "esprit commun", peut-être même d'un "inconscient collectif" qui s'est constitué de siècle en siècle. Et, très souvent, là où nous croyons exprimer nos propres idées, nous ne sommes que les porte-voix, les figurants d'un vaste "theatrum mundi" aux dimensions infinies.

  13. La radiothérapie du cancer du sein


    MEYNS, Mia


    Le traitement du cancer du sein est un des exemples les plus probants de l'approche multidisciplinaire oncologique. Dans ce contexte, la radiothérapie a renforcé sa place et son importance, même si certaines incertitudes persistent, comme la prise en charge du creux axillaire, de la chaîne mammaire interne et de la paroi thoracique. La tendance constatée depuis plusieurs années est celle d'une approche moins agressive. Peer reviewed

  14. Incidence du salaire minimum sur le marché du travail en Amérique ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Incidence du salaire minimum sur le marché du travail en Amérique centrale - une analyse comparative du Costa Rica, du Salvador et du Nicaragua ... The Impact of Minimum Wage on Labour Markets in Central America : A Comparative Analysis of Costa Rica, El Salvador and Nicaragua, 1 January 2008 - 30 June 2010.

  15. Experience in developing control integrated multilevel systems for gas transport; Developpement de systemes integres de gestion multi-niveaux pour le transport du gaz

    Energy Technology Data Exchange (ETDEWEB)

    Kostyukov, V.Y. [NIIIS, (Russian Federation); Bityukov, V.S. [Gasprom, (Russian Federation)


    This report describes the experience of the integrated control multilevel system (IACS) development and implementation for gas transport at the regional enterprises of JSC 'Gasprom', specificity of IACS creation by the Russian enterprises on the basis of the technical and licensed basic software SCADA Geamatics purchased from AEG company under the contract. (authors)

  16. Study of the Use of Antibiotics with ATC/DDD System and DU 90% in Digestive Surgery in Hospital in Bandung

    Directory of Open Access Journals (Sweden)

    Febrina Mahmudah


    Full Text Available Antibiotics are the most widely prescribed medicines in the hospital. Improper use of antibiotics can cause various problems, such as treatment will be more expensive, more toxic side effects, and the emergence of widespread resistance superinfection events that are difficult to treat. The objective of this study was to determine quantity and pattern of antibiotic usage in hospitalized patients at one hospital in Bandung. Sample were taken by simple random sampling method. The quantity of antibiotics usage was assessed by counting the Defined Daily Dose (DDD/100 patient days and DU 90%. Based on 208 patient medical records, it was found that total antibiotic usage was 17.9 DDD/100 patient days and antibiotics were included in the DU 90% is ceftriaxone (8.77 DDD/100 patient days, metronidazole (4.61 DDD/100 patient days, cefixime (1.09 DDD/100 patient days, cefepime (0.85 DDD/100 patient days, ciprofloxacin (0.73 DDD/100 patient days and meropenem (0.42 DDD/100 patient days. The results showed that the use of antibiotics in quantity at most is ceftriaxone for 8.77 DDD / 100 days of hospitalization and antibiotics included in segment 90% were ceftriaxone, metronidazole, cefixime, cefepime, ciprofloxacin, and meropenem.

  17. Jouer du piano

    Directory of Open Access Journals (Sweden)

    Fériel Kaddour


    Full Text Available La réflexion s’appuie dans un premier temps sur une opposition entre deux attitudes de pianistes  à l’égard du travail à l’instrument : Gould, qui revendique une séparation d’avec le clavier pour ne privilégier que la lecture; Arrau, dont la technique au contraire vise à « faire corps » avec son piano. L’étude de ces deux démarches d’interprètes conduit à une conclusion croisée : l’abstraction gouldienne n’est rien d’autre qu’un déplacement du jeu vers d’autres instruments (ceux qui servent à la prise de son et au montage de ses enregistrements ; le « faire-corps » hérité de la culture pianistique romantique est plus dialectique que fusionnel, et en cela implique une capacité de mise à distance. A partir de cette double conclusion, on tâche enfin de repenser la place du jeu à l’instrument dans la mise en œuvre d’une interprétation, en interrogeant le dialogue qui s’instaure entre la partition telle qu’elle s’écrit et le geste tel qu’il se joue.Our study leans on an opposition between two pianists' attitudes about their work with the instrument. Gould claims a necessary separation from the keyboard in order to prioritize reading. Arrau, on the contrary, relies on a technique which consists in “being one” with his piano. The analysis of these two interprets’ behaviours leads to a crossed conclusion: the gouldian abstraction is nothing else than a displacement of the playing towards another kind of instruments, the ones he uses in sound recording and cut up; Arrau’s “being one” is more dialectic than at first sight, and it therefore implies a real distancing from the piano. This constatation leads to rethink the place of the piano playing in the setting of an interpretation, and to highlight the real dialogue which develops itself between the score as it has been written and the gesture as it is played.

  18. Derivation of the low Mach number diphasic system. Numerical simulation in mono-dimensional geometry; Derivation du systeme diphasique bas Mach. Simulation numerique en geometrie monodimensionnelle

    Energy Technology Data Exchange (ETDEWEB)

    Dellacherie, St


    This work deals with the derivation of a diphasic low Mach number model obtained through a Mach number asymptotic expansion applied to the compressible diphasic Navier Stokes system, expansion which filters out the acoustic waves. This approach is inspired from the work of Andrew Majda giving the equations of low Mach number combustion for thin flame and for perfect gases. When the equations of state verify some thermodynamic hypothesis, we show that the low Mach number diphasic system predicts in a good way the dilatation or the compression of a bubble and has equilibrium convergence properties. Then, we propose an entropic and convergent Lagrangian scheme in mono-dimensional geometry when the fluids are perfect gases and we propose a first approach in Eulerian variables where the interface between the two fluids is captured with a level set technique. (author)

  19. Disposable Multi-Sensor Unattended Ground Sensor Systems for Detecting Personnel (Systemes de detection multi-capteurs terrestres autonome destines a detecter du personnel) (United States)


    the set of DCT coefficients for all the training data corresponding to the people. Then, the matrix ][ pX can be written as: ][][][ −+ −= ppp XXX ...deployed on two types of ground conditions. This included ARL multi-modal sensors, video and acoustic sensors from the Universities of Memphis and...Mississippi, SASNet from Canada, video from Night Vision Laboratory and Pearls of Wisdom system from Israel operated in conjunction with ARL personnel. This

  20. Le Brahmane du Komintern

    Directory of Open Access Journals (Sweden)

    Elizabeth Burgos


    Full Text Available Le Brahmane du Komintern, largometraje documental del realizador francés Vladimir León, constituye un ejercicio ejemplar de investigación histórica y  de lograda factura de realización. Y, pese a no haber contado con la ayuda de ninguno organismo público, se trata de un ambicioso proyecto que cubre una amplia extensión geográfica que abarca: Estados Unidos, México, Moscú, Berlín, y la India. Gira en torno a una figura que tuvo en su tiempo su hora de gloria. Un bengalí, hijo de braman, la c...

  1. Le Brahmane du Komintern


    Elizabeth Burgos


    Le Brahmane du Komintern, largometraje documental del realizador francés Vladimir León, constituye un ejercicio ejemplar de investigación histórica y  de lograda factura de realización. Y, pese a no haber contado con la ayuda de ninguno organismo público, se trata de un ambicioso proyecto que cubre una amplia extensión geográfica que abarca: Estados Unidos, México, Moscú, Berlín, y la India. Gira en torno a una figura que tuvo en su tiempo su hora de gloria. Un bengalí, hijo de braman, la ca...

  2. Le sacre du printemps

    Directory of Open Access Journals (Sweden)

    Denise Pumain


    Full Text Available Cybergeo aura six ans en avril : dans la réalité du virtuel, dans l'univers récent et fluctuant de la publication en ligne, cela fait de nous, tout à la fois, des pionniers et des vétérans. De façon plus surprenante, il se trouve que nous sommes aussi uniques : parmi toutes les revues électroniques de sciences sociales, aucune ne combine comme Cybergeo ancienneté, publication exclusivement électronique, liberté d'accès au texte intégral, édition et gestion par des chercheurs, et comité de lec...

  3. Les espaces du travail


    Bach, Caroline; Bachet, Daniel; Benedetto-Meyer, Marie; Bonnet, Estelle; Bonnet, Thomas; Bouffartigue, Paul; Cihuelo, Jérôme; Clot, Yves; Duflos, Julie; Durand, Jean-Pierre; Fortino, Sabine; Jacquot, Lionel; Kirov, Vassil; LANDOUR, Julie; Le Petitcorps, Colette


    L’espace de travail prend depuis le début des années 1990 une importance nouvelle au sein des entreprises et des administrations dont traite le Corpus de ce numéro. Il semble (re)devenir une composante stratégique des politiques de réorganisation et de réduction des coûts. Au-delà de cette actualité, l’espace s’apparente à un instrument durable utilisé par les directions d’entreprise pour structurer et contrôler le travail. En effet, l’organisation du travail et l’organisation spatiale entret...

  4. Utilisation du semoir philippin, une alternative au repiquage du riz ...

    African Journals Online (AJOL)

    Les avantages tirés de l'utilisation de cet outil sont multiples : économie du temps de travail, homogénéité de répartition des semences, levée uniforme des plantules de riz. En plus, l'utilisation du semoir philippin permet d'économiser les semences de près de 50 % par rapport au repiquage, et d'environ 75 % par rapport ...


    Directory of Open Access Journals (Sweden)

    Gholamreza Djelveh


    Full Text Available Mousses alimentaires sont un sous-ensemble des aliments connus sous le nom de produits fouettés ou des produits aérés. Ils sont des produits formulés avec des qualités telles que la légèreté et la souplesse et sont principalement consommés à l'apéritif ou au dessert. Les produits en mousse obtenue par dispersion d'un gaz dans une matrice alimentaire (la phase continue ont connu un développement croissant au cours des années 80 et 90. Le processus d'aération liés à leurs activités de production est appelée l'expansion ou à fouetter. Le document présente les principaux-paramètres du procédé du point permanent de la formulation, la mise en œuvre processus dans les installations pilotes et à l'échelle industrielle, la caractérisation des produits finis, la base énergétique de l'échelle de processus en place, et le lien entre la formulation, émulsion préparation de l'expansion. Cette vue d'ensemble de l'opération d'expansion continue, nous a permis de mettre en évidence le fait qu'il ya des opérations de l'unité encore mal décrite par le génie des procédés et pour lesquels les méthodes et outils pour l'extrapolation et la prédiction sont encore à leurs balbutiements.

  6. The consideration of the humane factor is essential in safety systems; La prise en compte du facteur humain est primordiale dans les systemes de securite

    Energy Technology Data Exchange (ETDEWEB)

    Parisot, F.


    In most risk analysis we consider that the staff fit perfectly the tasks to do in terms of training and competence but in fact a lot of factors intervene like the level of stress of the operator, the time available to identify the trouble or to take a decision, the relevance of the procedures, or the level of coordination and communication between the members of the staff. Different methods exist to assess the human factor, most have been designed to be used in the nuclear sector for instance: THERP (Technique for Human Error Rate Prediction) or OATS (Operation Action Tree) or SHARP (Systematic Human Action Reliability Procedure). These methods apply as early as the design stage of the engineered safety systems. Virtual reality has entered these methods because it allows operators to learn by making errors since errors in virtual reality have no consequences. Learning by making errors is an efficient method to get the operator used to accidental situations and as a consequence to reduce his level of stress. Some methods incorporate human elements into system safety analysis through the definition of performance shaping factors that describe the behaviour of operators in terms of physical and psychological abilities. (A.C.)

  7. Alimentation du nouveau-ne et du nourrisson dans la region ...

    African Journals Online (AJOL)

    Alimentation du nouveau-ne et du nourrisson dans la region centrale du togo : pratiques familiales et communautaires avant la mise en oeuvre de la strategie « prise en charge integree des maladies de l'enfant »

  8. Portable multi-sensor system for gas detection using the temporal window technique; Systeme multicapteurs de detection de gaz, portable, utilisant la technique du fenetrage temporel

    Energy Technology Data Exchange (ETDEWEB)

    Cazaubon, Ch. [Bordeaux-1 Univ., CRED, 33 - Talence (France); Levi, H.; Bordieu, Ch.; Rebiere, D.; Pistre, J. [Bordeaux-1 Univ., Lab. IXL, UMR CNRS 5818, 33 (France)


    An autonomous and portable multi-sensor system was constructed. It can drive four gas sensors (surface acoustic waves. SAW. for examples) and four voltage output gas sensors (semiconductor metal oxide sensors, for example). Two micro-controllers. MC68HC11F1 and MC68HC711E9, used as master and slave respectively, are mounted on two cards. The first card contains the signal processing treatment algorithm using a neural network and a shifting temporal window technique: it allows real time gas selection. The second card insure the overall temperature control by an auto-adaptive PID. GB gas SAW responses were applied to the device in order to test his performances. (authors)

  9. The role of the nuclear industry in the Brazil's electricity system; Le role du nucleaire dans le systeme electrique Bresilien

    Energy Technology Data Exchange (ETDEWEB)

    Pinheiro Da Silva, O.L.; Dos Santos Guimaraes, L. [Electronuclear - Electrobras Termonuclear (Brazil)


    No form of energy - renewable, clean or whatever other name it is given - can constitute the sole solution to the challenge of ensuring electric power supplies that are compatible with a country's economic and social growth. Brazilian consumption and installed electrical generation capacity per capita are stiller low, below the world average - that is the main factor that needs to be stressed. The catch-up requires that the country benefit it as much as possible and as soon as possible from all available sources to increase electricity generation capacity allowing increased consumption compatible with the way of life that Brazilians aspire to. A country's electrical system planning requires the efficient management of a diversified array of energy sources. Regarding Brazil, hydro power will be or several decades the main component in its electricity generation portfolio. Nonetheless, it already needs to be complemented by thermal sources - uranium, coal, bio-mass, natural gas and petroleum-based oil - in that order of importance, taking account of such actors as availability on the home territory, costs, environmental impacts and uses in other applications. (authors)

  10. Annex 2: Simulation of the whole system - Calibration using measured data; Annexe 2: simulation du systeme complet et calibrage sur les mesures

    Energy Technology Data Exchange (ETDEWEB)

    Pahud, D.


    This is the Annex 2 of the annual report 2006 of the Serso project. The basic idea of the Serso project is to defrost a road bridge in winter with heat produced by the bridge in summer by solar radiation absorption and stored in a diffusive underground store. Pipes are imbedded in the bridge surface material and connected to a set of underground borehole heat exchangers ('geothermal probes'). A heat transfer fluid is circulated by a pump. The store is loaded in summer and discharged in winter. In the present Annex the mathematical model developed to simulate the whole system is described. It includes the validated simulation model of the underground heat store (see Annex 1). The parameters of the model are tuned on the basis of a two-years series of field measurements. Although the measured quantities do not include essential variables like the sky temperature and the bridge model is one-dimensional, the model satisfactorily describes the energy balance of the bridge.

  11. Sur quelques Ichthyurus du Tonkin

    NARCIS (Netherlands)

    Gestro, R.


    Les espèces d Ichthyurus du Tonkin connues jusqu’ici étaient seulement trois: denticornis Gestro, décrite d’abord du Tenasserim (récoltes de feu Mr. L. Fea) et retrouvée ensuite dans le Haut Tonkin par S. A. R. le Prince Henri d’Orléans; Henrici Gestro, découverte par ce même explorateur, et dont le

  12. Cas du CRZ de Dahra

    African Journals Online (AJOL)


    30 avr. 2015 ... Le Ferlo, comme la plupart des zones semi-arides de l'Afrique occidentale, est affecté par les séries de ... du climat à travers la séquestration du carbone dans les sols (Soussana et al., 2007 ; 2010). Ils ... Climat, sols, végétation et systèmes de production. Le climat : La pluviométrie varie d'une année à l' ...

  13. Charte du Conseil des Gouverneurs

    International Development Research Centre (IDRC) Digital Library (Canada)

    Office 2004 Test Drive User

    Le Centre de recherches pour le développement international (CRDI) est une société d'État créée par le Parlement du Canada en 1970. Il fait rapport au Parlement par l'entremise du ministre désigné par le gouverneur en conseil, à savoir le ministre des Affaires étrangères à l'heure actuelle. La Loi sur le Centre de ...

  14. Alexip: an Expert System Coupled with a Dynamic Simulator for the Supervision of the Alphabutol Process ALEXIP : système couplé à un simulateur dynamique pour la supervision du procédé Alphabutol

    Directory of Open Access Journals (Sweden)

    Cauvin S.


    Full Text Available ALEXIP (ALphabutol EXpert IFP is a knowledge-based system designed to improve the operation of the Alphabutol petrochemical process (IFP process which produces 1-butene by dimerizing ethylene by helping the operators in their process control tasks in running industrial units. Coupled with a dynamic simulator, this system assesses the situation of the process at any time and determines the measures to be taken to maintain it or to bring it into a state of optimal operation. The structure developed is generic. The knowledge is stored in the form of facts easily read by the experts and processed by general rules written in second-order logic using the Genesia 2 software. It accordingly helps to accommodate new knowledge easily, and can be adapted to other processes. ALEXIP (ALphabutol Expert IFP est un système à base de connaissances dont le but est d'améliorer l'exploitation du procédé pétrochimique Alphabutol (procédé IFP qui produit du butène-1 par dimérisation de l'éthylène en aidant les opérateurs dans leurs tâches de conduite des unités industrielles. Couplé à un simulateur dynamique, ce système analyse l'état dans lequel se trouve le procédé à un instant donné et détermine les actions à entreprendre pour le maintenir ou le ramener dans un état de fonctionnement optimal. La structure élaborée est générique ; la connaissance est stockée sous forme de faits lisibles facilement par les experts et traités par des règles générales écrites en logique d'ordre 2 à l'aide du logiciel Génésia 2. Elle permet donc d'accueillir facilement de nouvelles connaissances et peut être adaptée à d'autres procédés.

  15. batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

    CERN Multimedia

    Valeriane Duvivier


    batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

  16. Sud du Sahara | Page 55 | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Sud du Sahara. Sud du Sahara. Read more about Strengthening the Health System through a Maternal Death Review (Kenya and Zimbabwe). Langue English. Read more about Taxes sur les cigarettes en Tanzanie. Langue French. Read more about Cigarette Taxation in Tanzania. Langue English. Read more about AIDS ...

  17. Etat Du Magnesium Dans Quelques Sols Sales Du Sud Et Du ...

    African Journals Online (AJOL)

    étude a été réalisée sur 86 échantillons de sols provenant du Centre et du Sud de l´Irak. L\\'expérimentation a consisté, d´une part, à calculer les différents sels dominants dans les sols salés par la méthode de combinaison hypothétique et, ...

  18. Le bal du loup

    CERN Document Server

    Happy Children's Home


    The Bord'eau amateur theatre group will graciously perform a play of their creation Le bal du loup Saturday 19 October 2013 at 20:00 Sunday 20 October at 17:00 in the Théâtre des Grottes Rue Louis Favre 43, 1201 Genève Children from age 12 upwards. Summary: The new-elected mayoress of a small village would like to clean up the town by prohibiting alcohol and getting rid of its prostitutes. Then along comes « Massimo Lupo » the pimp... The performances will be given to support the Happy Children's Home charity, which runs a foster-home in Pokhara for Nepali children: Admission : minimum charge of 10 CHF per person requested, to cover the cost of technical assistance and theatre rental. Any profit will be used solely for the foster-home. At the end of each performance members of the HCH charity will be happy to answer any questions you may have. The theatre has 86 seats, thank you for reserv...

  19. Cascade: a Knowledge-Based System Supporting Shoe Depths Selection and Casing Design CASCADE : un système à base de connaissances pour l'aide au choix de la profondeur des sabots et à la conception du tubage

    Directory of Open Access Journals (Sweden)

    Sansone A.


    meet all the requirements and minimize cost is not a trivial task. The choice of the expert systems technology exploited for the system development is related to the following topics :(a An expert system can combine several different skills. (b It guarantees easy and modular expanclibility with the acquisition of new experience. (c Moreover, new experience must be taken into account in order to continuously increase the quality of the results. (d Available data come from many sources, and all have to be considered when choosing the shoe depths' and casing's geometrical and mechanical characteristics. (e An expert system can explicitly represent both domain and strategical knowledge that can easily be modified, added or expunged. It can also give the user a feedback on its own behaviour, and it can also be used as a training tool. The benefits for the final user are:(a An efficient, integrated environment providing the tools the drilling engineer needs to work with. (b Greater accuracy. (c Access and assessment of knowledge and procedures regarding this matter. (d Interactive, user friendly interface. CASCADE est un système d'aide à la conception du tubage et au choix des sabots pour les puits d'exploration et de production. Ce système expert est une partie du projet ADIS (Advanced Drilling Information System, Système d'Information Avancé pour le Forage développé en commun par trois sociétés du groupe ENI : AGIP, ENIDATA et SAIPEM. Le but d'ADIS est de fournir des outils informatiques d'ingénierie de forage pour la planification et le suivi des opérations, utilisables aussi bien sur le site de forage que dans les bureaux. Ces outils devront également superviser les opérations en temps réel et émettre des diagnostics. Pour les tâches de suivi en temps réel, l'équipe de projet d'ADIS a mis au point des systèmes permettant la communication par satellite entre les appareils de forage et la base, ainsi que des systèmes experts de supervision pour l

  20. Speciation chimique du plomb et du cuivre par extraction ...

    African Journals Online (AJOL)

    La démarche méthodologique a consisté à un échantillonnage, à une extraction du plomb et du cuivre dans l'eau et dans les sédiments prélevés et au dosage de ces métaux à l'aide d'un spectrophotomètre d'absorption atomique à flamme. La méthode d'extraction séquentielle utilisée a été celle de Tessier. Les résultats ...

  1. Effet Du Potassium Et Du Deficit Hydrique Modere Sur La ...

    African Journals Online (AJOL)

    Le comportement de 6 génotypes de cette plante, sous deux régimes hydriques, avec ou sans apport de potassium, a été suivi dans des tubes en PVC. Le potassium a été apporté sous forme de KCl ou de K2SO4. Il n\\'y a pas de différence entre les effets du KCl et du K2SO4 sur la transpiration par mg de feuilles sèches ...

  2. La reconstruction du sourcil par greffon composite du cuir chevelu ...

    African Journals Online (AJOL)

    Les sourcils jouent un rôle important dans l'équilibre esthétique du visage. Leur reconstruction ou ophriopoïése, après séquelle de brûlure fait partie intégrante du programme de réhabilitation de la face brûlée. Plusieurs techniques ont été décrites. Nous insistons ici sur l'intérêt d'une technique simple, à la portée de tous ...

  3. Dynamique des populations du foreur des tiges du cacaoyer ...

    African Journals Online (AJOL)


    30 nov. 2014 ... RESUME. Objectif : Le foreur de tiges du cacaoyer, Eulophonotus myrmeleon Felder cause aujourd'hui d'énormes dégâts dans les cacaoyères ivoiriennes. La présente étude vise à déterminer les périodes de fortes attaques de ce déprédateur dans la région du Haut-Sassandra, la deuxième plus grande ...

  4. Continuous improvement journey at Du Pont photomasks (United States)

    Henderson, Robert K.


    This paper describes the history and experiences of Du Pont Photomasks in their efforts to integrate the continuous improvement philosophy and practices embodied in the Malcolm Baldrige National Quality Award criteria into their way of doing business. A case study of key learnings in this almost four year long process is presented. Specific topics discussed include the process applied to achieve ISO 9000 certification, the quality systems deployed in this effort, and the use of a balanced set of business and quality metrics to assess and improve upon performance.

  5. Main: 1DU5 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1DU5 トウモロコシ Corn Zea mays L. Zeamatin Precursor. Name=Zlp; Zea Mays Molecule: Zeama...NNACPVFKKDEYCCVGSAANDCHPTNYSRYFKGQCPDAYSYPKDDATSTFTCPAGTNYKVVFCP corn_1DU5.jpg ...

  6. Cri du Chat syndrome

    Directory of Open Access Journals (Sweden)

    Cerruti Mainardi Paola


    Full Text Available Abstract The Cri du Chat syndrome (CdCS is a genetic disease resulting from a deletion of variable size occurring on the short arm of chromosome 5 (5p-. The incidence ranges from 1:15,000 to 1:50,000 live-born infants. The main clinical features are a high-pitched monochromatic cry, microcephaly, broad nasal bridge, epicanthal folds, micrognathia, abnormal dermatoglyphics, and severe psychomotor and mental retardation. Malformations, although not very frequent, may be present: cardiac, neurological and renal abnormalities, preauricular tags, syndactyly, hypospadias, and cryptorchidism. Molecular cytogenetic analysis has allowed a cytogenetic and phenotypic map of 5p to be defined, even if results from the studies reported up to now are not completely in agreement. Genotype-phenotype correlation studies showed a clinical and cytogenetic variability. The identification of phenotypic subsets associated with a specific size and type of deletion is of diagnostic and prognostic relevance. Specific growth and psychomotor development charts have been established. Two genes, Semaphorin F (SEMAF and δ-catenin (CTNND2, which have been mapped to the "critical regions", are potentially involved in cerebral development and their deletion may be associated with mental retardation in CdCS patients. Deletion of the telomerase reverse transcriptase (hTERT gene, localised to 5p15.33, could contribute to the phenotypic changes in CdCS. The critical regions were recently refined by using array comparative genomic hybridisation. The cat-like cry critical region was further narrowed using quantitative polymerase chain reaction (PCR and three candidate genes were characterised in this region. The diagnosis is based on typical clinical manifestations. Karyotype analysis and, in doubtful cases, FISH analysis will confirm the diagnosis. There is no specific therapy for CdCS but early rehabilitative and educational interventions improve the prognosis and considerable

  7. Gestion durable du paysage

    Directory of Open Access Journals (Sweden)

    Jean-David Gerber


    Full Text Available Le paysage est de plus en plus perçu comme une ressource. À ce titre, il est nécessaire de trouver des instruments juridiques, politiques ou économiques susceptibles de gérer cette « ressource-paysage » sur le long terme. Le gouvernement suisse a introduit récemment l’instrument des parcs naturels régionaux, organisés selon le modèle français, dans sa législation de protection de la nature et du paysage. Une mise en regard des nouveaux parcs avec des structures de gestion beaucoup plus anciennes, les bourgeoisies et les corporations, permet de mettre en évidence les forces et les faiblesses de chacun de ces instruments dans leur contribution à résoudre les rivalités d’usage entre acteurs utilisant ou influençant la ressource paysage. Cette comparaison permet de formuler des recommandations pratiques concernant la gestion de cette ressource.The landscape is increasingly perceived as a resource. For this reason, it is necessary to find legal, political and economic instruments that will succeed in managing this “resource landscape” in the long term. The Swiss government recently introduced the instrument of regional nature parks into the legislation governing nature and landscape preservation; the proposed parks are organized on the basis of the French model. The examination of the new parks from the perspective of much older management structures, i.e. the civic municipalities (bourgeoisies and corporations, makes it possible to demonstrate the strengths and weaknesses of each of these instruments in their contribution to the resolution of use rivalries between actors who use or influence the resource landscape. This comparison also enables the formulation of practical recommendations regarding the management of this resource.

  8. Gouvernance communautaire des milieux humides du bassin ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Gouvernance communautaire des milieux humides du bassin versant de la Sand River (Afrique du Sud). La collectivité de Craigieburn, dans la province du KwaZulu-Natal, en Afrique du Sud, est située dans un milieu humide couvrant 1 200 ha et jouant un rôle de premier plan dans la régulation et le maintien de la Sand ...

  9. Introduction : Approches plurielles du quotidien

    Directory of Open Access Journals (Sweden)

    La Rédaction d'Articulo - revue de sciences humaines


    Full Text Available Ce premier numéro d' poursuit la réflexion ouverte dans la présentation générale autour du quotidien et sa restitution dans des approches globales. Chacun des textes rassemblés ici cherche à transcender une conception dominante, purement tautologique de la quotidienneté comme raison suffisamment «évidente» d'une observation empirique isolée du reste, et tente ainsi d'atteindre des dimensions plus profondes et de valeurs différentes. En somme, les textes rassemblés ici cessent de chanter l’essence du quotidien, l’universalité des petits faits de nature, au profit de sa fonction signifiante en un ensemble de cadres d’analyses.

  10. extérieur du canada

    International Development Research Centre (IDRC) Digital Library (Canada)

    Hakan Mustafa

    RENSEIGNEMENTS GÉNÉRAUX, FISCAUX ET BANCAIRES DU FOURNISSEUR –. EXTÉRIEUR DU CANADA. Veuillez lire attentivement les instructions figurant aux pages 2 et 3 avant de remplir le présent formulaire. Nouveau. Modification. Section 1 : RENSEIGNEMENTS GÉNÉRAUX. Nom du particulier (nom, prénom) ...

  11. Multifragmentation of a heavy system: study of the system Gd + U at 36 MeV per nucleon with INDRA; Multifragmentation d`un systeme lourd: etude avec INDRA du systeme Gd + U a 36 MeV par nucleon

    Energy Technology Data Exchange (ETDEWEB)

    Squalli-Houssaini, M.


    In this work we looked for signatures of the multifragmentation process induced in a very heavy system. The products of the reaction Gd+U at 36 AMeV were detected with the multidetector INDRA. Events where more than 80 per cent of the total charge of the system and more than 75 percent of the incident linear momentum were recorded could be selected thanks to the exceptional performances of INDRA. A composite system comprising almost all the nucleons of the incident nuclei was shown to be formed with a cross section of {approx} 22 mb. This system emits isotropically a large number of fragments and light particles. Comparisons with dynamical and statistical simulations show that a small quantity of particles is emitted early (preequilibrium), but that the break-up of the system into fragments is very rapid (multifragmentation), but that the break-up of the system into fragments is very rapid (multifragmentation); it occurs while the system is expanding as an extra radial energy has to be introduced in order to reproduce the kinematical observables. Moreover the heaviest fragment of each partition systematically presents a lower velocity, which could mean that it is located close to the center of the system. (author).

  12. Établissement du programme de recherche 2012-2014 du réseau d ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Établissement du programme de recherche 2012-2014 du réseau d'analyse du développement de la sous-région du Grand Mékong. Le Greater Mekong Subregion-Development Analysis Network (GMS-DAN) est un réseau de recherche au service du développement concertée qui réunit les principaux instituts de ...

  13. L'Aventure du LHC

    CERN Document Server

    CERN. Geneva


    Cette présentation s’adressera principalement aux personnes qui ont construit le LHC. La construction du LHC fut longue et difficile. De nombreux problèmes sont apparus en cours de route. Tous ont été résolus grâce au dévouement et à l’engagement du personnel et des collaborateurs. Je reviendrai sur les coups durs et les réussites qui ont marqués ces 15 dernières années et je vous montrerai combien cette machine, le fruit de vos efforts, est extraordinaire.

  14. Introduction : Approches plurielles du quotidien


    La Rédaction d'Articulo - revue de sciences humaines


    Ce premier numéro d' poursuit la réflexion ouverte dans la présentation générale autour du quotidien et sa restitution dans des approches globales. Chacun des textes rassemblés ici cherche à transcender une conception dominante, purement tautologique de la quotidienneté comme raison suffisamment «évidente» d'une observation empirique isolée du reste, et tente ainsi d'atteindre des dimensions plus profondes et de valeurs différentes. En somme, les textes rassemblés ici cessent de ch...

  15. utilisation du semoir philippin, une alternative au repiquage du riz ...

    African Journals Online (AJOL)

    Since 2008, a pre-germinated seedling equipment named Filipino seed drill has been introduced and tested in the Office du Niger. The results of the station and on-farm adaptation tests from 2009 to 2012 showed that this equipment, under specific conditions, can validly replace rice transplanting without yield loss. Average ...

  16. Poumon du puisatier (United States)

    Elidrissi, Amal Moustarhfir; Zaghba, Nahid; Benjelloun, Hanane; Yassine, Najiba


    Le puisatier a pour profession le creusement et l'entretien des puits pour fournir de l'eau. Il est au contact de divers minerais, particulièrement la silice, particule qui présente un risque certain de développement des maladies pulmonaires connues sous le nom de silicose. Le but de notre travail est de préciser le profil épidémiologique, clinique, radiologique et évolutif des patients puisatiers silicotiques. C'est une étude rétrospective concernant 54 cas de puisatiers ayant une silicose, colligés au service des maladies respiratoires du CHU Ibn Rochd de Casablanca, de Mars 1997 à Janvier 2016. Tous les malades étaient des puisatiers, de sexe masculin, avec une moyenne d'âge de 50 ans. Le tabagisme était retrouvé dans 36 cas et un antécédent de tuberculose était noté dans huit cas. La radiographie thoracique retrouvait des grandes opacités dans 39 cas, des petites opacités dans 15 cas, et un épaississement des septats dans 11 cas. Ce tableau de silicose s'était compliqué d'une surinfection bactérienne dans 37% des cas, d' un pneumothorax dans 4% des cas et d'une tuberculose dans 20% des cas. La prise en charge thérapeutique était celle des complications. La déclaration de la maladie professionnelle et de l'indemnisation était faite. L'évolution était bonne dans 12 cas, stationnaire dans 17 cas et mauvaise dans 16 cas. La silicose est une pneumoconiose fréquente chez les puisatiers. Elle retentit sur la fonction respiratoire. Nous soulignons l'association fréquente de tuberculose et nous insistons sur la prévention qui reste le meilleur traitement. PMID:28292119

  17. Réflexion sur l’origine du processus de segmentation du marche du travail

    Directory of Open Access Journals (Sweden)

    Attia Nicole


    Full Text Available (francuski Ce travail propose une réflexion sur l'origine du processus de segmentation du marché du travail par rapport à l'entreprise. Se situe-t-elle au sein même de l'entreprise ou en amont, c'est à dire entre les entreprises? Cela revient à se demander si on peut avoir une approche microéconomique ou macroéconomique de la segmentation et, à s'interroger sur le rôle réel tenu par les firmes dans le processus. Déterminant pour la théorie, ce rôle est à repenser selon la réponse apportée à notre question.

  18. Du flux et du reflux de l'usage du tabac | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    14 déc. 2010 ... Le CRDI, grâce à des fonds de Santé Canada, appuie une étude économique qui aide le gouvernement de la Jamaïque à réfuter les arguments de ... Faculté des sciences économiques à l'Université du Cap, en collaboration avec Eva Lewis-Fuller et ses collègues du ministère de la Santé de la Jamaïque.

  19. The Pic du Midi solar survey (United States)

    Koechlin, L.


    We carry a long term survey of the solar activity with our coronagraphic system at Pic du Midi de Bigorre in the French Pyrenees (CLIMSO). It is a set of two solar telescopes and two coronagraphs, taking one frame per minute for each of the four channels : Solar disk in H-α (656.28 nm), prominences in H-α, disk in Ca II (393.3 nm), prominences in He I (1083 nm), all year long, weather permitting. Since 2015 we also take images of the FeXIII corona (1074.7 nm) at the rate of one every 10 minutes. These images cover a large field: 1.25 solar diameter, 2k*2K pixels, and are freely downloadable form a database. The improvements made since 2015 concern an autoguiding system for better centering of the solar disk behind the coronagraphic masks, and a new Fe XIII channel at λ=1074.7 nm. In the near future we plan to provide radial velocity maps of the disc and polarimetry maps of the disk and corona. This survey took its present form in 2007 and we plan to maintain image acquisition in the same or better experimental conditions for a long period: one or several solar cycles if possible. During the partial solar eclipse of March 20, 2015, the CLIMSO instruments and the staff at Pic du Midi operating it have provided several millions internet users with real time images of the Sun and Moon during all the phenomenon.

  20. application du Global Entrepreneurship Monitor

    International Development Research Centre (IDRC) Digital Library (Canada)

    'emplois en Asie du Sud-Est. Cependant, peu d'études empiriques ont été effectuées sur l'activité entrepreneuriale, l'innovation et les types de ... Entrepreneurship has been a major driver of growth and job creation in Southeast Asia.

  1. Cri-du-chat syndrome

    NARCIS (Netherlands)

    Didden, H.C.M.; Curfs, L.M.G.


    Cri-du-chat syndrome is a genetic disease resulting from a deletion occurring on the short arm of chromosome 5 (5p–). The incidence ranges from 1:15 000 to 1:50 000 live-born infants. Its main clinical features are a high-pitched monochromatic cry, microcephaly, broad nasal bridge, epicanthal folds,

  2. Project of decree relative to the licensing and statement system of nuclear activities and to their control and bearing various modifications of the public health code and working code; Projet de decret relatif au regime d'autorisation et de declaration des activites nucleaires et a leur controle et portant diverses modifications du code de la sante publique et du code du travail

    Energy Technology Data Exchange (ETDEWEB)



    This decree concerns the control of high level sealed radioactive sources and orphan sources. It has for objective to introduce administrative simplification, especially the radiation sources licensing and statement system, to reinforce the control measures planed by the public health code and by the employment code, to bring precision and complements in the editing of several already existing arrangements. (N.C.)

  3. Characterization of gastrin-cholecystokinin 2 receptor interaction in relation to c-fos induction

    NARCIS (Netherlands)

    Hollestelle, M.J.; Timmerman, P.; Meloen, R.H.; Höppener, J.W.M.


    The interaction of gastrin with the cholecystokinin 2 (CCK2)/gastrin receptor has been studied extensively in relation to gastric acid secretion. However, not much is known about the contribution of individual amino acids of gastrin interacting with the CCK2 receptor, when gastrin is acting as a

  4. Acute phencyclidine administration induces c-Fos-immunoreactivity in interneurons in cortical and subcortical regions

    DEFF Research Database (Denmark)

    Hervig, Mona E; Thomsen, Morten S; Kalló, Imre


    Dysfunction of N-Methyl-d-aspartate receptors (NMDARs) is believed to underlie some of the symptoms in schizophrenia, and non-competitive NMDAR antagonists (including phencyclidine (PCP)) are widely used as pharmacological schizophrenia models. Furthermore, mounting evidence suggests that impaired...


    Directory of Open Access Journals (Sweden)

    Ivan Jurković


    Full Text Available Les auteurs ont dćcrit l:i stratigiaphie. I.i tectonique, le corps de minerals, la paragenese et la genese dcs gttes Koudiat Sidri situ&s environ 20 km au sud de la ville du Kef en Tunisie.

  6. Etude de l'affaiblissement du comportement mecanique du pergelisol du au rechauffement climatique (United States)

    Buteau, Sylvie

    Le rechauffement climatique predit pour les prochaines decennies, aura des impacts majeurs sur le pergelisol qui sont tres peu documentes pour l'instant. La presente etude a pour but d'evaluer ces impacts sur les proprietes mecaniques du pergelisol et sa stabilite a long terme. Une nouvelle technique d'essai de penetration au cone a taux de deformation controle, a ete developpee pour caracteriser en place le pergelisol. Ces essais geotechniques et la mesure de differentes proprietes physiques ont ete effectues sur une butte de pergelisol au cours du printemps 2000. Le developpement et l'utilisation d'un modele geothermique 1D tenant compte de la thermodependance du comportement mecanique ont permis d'evaluer que les etendues de pergelisol chaud deviendraient instables a la suite d'un rechauffement de l'ordre de 5°C sur cent ans. En effet, la resistance mecanique du pergelisol diminuera alors rapidement jusqu'a 11,6 MPa, ce qui correspond a une perte relative de 98% de la resistance par rapport a un scenario sans rechauffement.

  7. By-law from December 12, 2005 relative to nuclear pressure systems; Arrete du 12 decembre 2005 relatif aux equipements sous pression nucleaires

    Energy Technology Data Exchange (ETDEWEB)



    This by-law deals with the specifications relative to the design, fabrication and conformability evaluations of nuclear pressure system, i.e. systems that may lead to a radioactive contamination in the case of failure. The main safety requirements of these systems are recalled in appendixes (nondestructive testing and qualification). (J.S.)

  8. Participation du patient Marocain atteint du cancer au choix ...

    African Journals Online (AJOL)

    Introduction: La décision médicale partagée 'Shared decision making' est un concept qui se développe depuis les années 1990. Il donne aux patients le soutien nécessaire pour exprimer leurs préférences et partager la décision médicale. Cette étude cherche à estimer le degré de participation du patient Marocain atteint ...

  9. Caractérisations agromorphologique et anatomique du gombo du ...

    African Journals Online (AJOL)

    1Laboratoire de botanique et écophysiologie, UFR/SVT, Université de Ouagadougou. 2Université de Ouagadougou, Laboratoire de génétique et biotechnologies des végétaux, UFR/SVT, Université de Ouagadougou. 3Directrice du Laboratoire de botanique et écophysiologie, UFR/SVT, Université de Ouagadougou.

  10. Le Centre du Mali : épicentre du djihadisme?

    NARCIS (Netherlands)

    Sangaré, B.


    La multiplication d'épisodes violents dans le centre du Mali, depuis la moitié de l'année 2015, démontre à quel point la paix reste fragile dans ce pays. La signature des accords pour la paix et l'amorce d'un processus de réconciliation ont certes marqué une avancée pour le gouvernement, mais la

  11. Gestion du parc agroforestier du terroir de Vipalogo (Burkina Faso ...

    African Journals Online (AJOL)

    Au Burkina Faso, l'arbre rural fait partie des systèmes de production. Le choix des espèces, leur densité, les modes de gestion ainsi que les besoins à satisfaire, obéissent à des critères propres aux producteurs. C'est pour comprendre ces logiques paysannes de la gestion du parc agroforestier que la présente étude a été ...

  12. Effet du Pediococcus acidilactici sur le bilan lipidique sanguin du ...

    African Journals Online (AJOL)

    Pour évaluer l'effet du probiotique Pediococcus acidilactici, nous avons utilisé mille sept cent soixante-trois poussins d'un jour de l'espèce Gallus domesticus, appartenant à la souche Hubbard F15, de sexes mélangés et d'un poids homogène, provenant d'un même couvoir, mis en place dans le même bâtiment pour être ...

  13. L’Harmonie du monde

    Directory of Open Access Journals (Sweden)

    Martine Clouzot


    Full Text Available La Bourgogne est particulièrement bien présente et représentée dans l’exposition sur la musique et ses représentations au Moyen Âge organisée par Isabelle Marchesin (université de Poitiers, Christine Laloue (conservatrice du Patrimoine au Musée et Martine Clouzot (université de Bourgogne, au Musée de la Musique à Paris du 26 mars au 27 juin 2004. En Côte-d’Or, à Dijon, la Bibliothèque municipale a donné son accord officiel pour le prêt de la Bible d’Etienne Harding, les Moralia in Job et u...

  14. L'energie du moustique

    CERN Multimedia

    Augereau, J F


    ENSEMBLE DE QUATRE ARTICLES - LARGE HADRON COLLIDER: Le dernier accelerateur de particules du CERN, le LEP, produisait des faisceaux d'electrons de 100 GeV chacun. Le LHC, qui accelere des faisceaux de protons, leur communiquera une energie de 7 TeV chacun. Une energie a la fois colossale et derisoire. Un TeV represente a peu pres l'energie cinetique d'un moustique (1/2 page).

  15. Le Developmental Entrepreneurship Program du Massachusetts ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Réseau sur l'économie du savoir dans les Caraïbes. Le CRDI soutient actuellement deux projets associés à l'initiative portant sur l'économie du savoir dans les Caraïbes (Caribbean Knowledge Economy, CKE), à savoir Avantages découlant de... Voir davantageRéseau sur l'économie du savoir dans les Caraïbes ...

  16. Carcinome verruqueux du larynx : Diagnostic et traitement ...

    African Journals Online (AJOL)

    Introduction : Les carcinomes verruqueux du larynx sont des tumeurs rares. Ils posent des problèmes diagnostics et thérapeutiques. Le but de notre travail est de détailler les difficultés diagnostiques et thérapeutiques du carcinome verruqueux du larynx. Patients et méthodes : Treize malades ont été traité d'un carcinomes ...

  17. Annonce du diagnostic et rupture biographique

    African Journals Online (AJOL)

    du cancer en Tunisie , maladie chronique identifiée parmi les plus répandues et les plus mortelles , la première place des ... du Cancer du Sud Tunisien , paru en l'an 2000 , concernant les années 1997-1999, l'incidence globale ...... in Olivier Fillieule et Nonna Mayer, (dir) dossier “Devenirs Militants”,. Revue française de ...

  18. Effets du travail du sol sur le comportement chimique et biologique ...

    African Journals Online (AJOL)

    Effets du travail du sol sur le comportement chimique et biologique du sol et les conséquences sur le rendement d'un blé tendre (Triticum aestivum L.) dans la région « non chernozem » de la fédération de Russie.

  19. Genetics Home Reference: cri-du-chat syndrome (United States)

    ... Health Conditions Cri-du-chat syndrome Cri-du-chat syndrome Printable PDF Open All Close All Enable ... view the expand/collapse boxes. Description Cri-du-chat (cat's cry) syndrome, also known as 5p- (5p ...

  20. Caractérisation des sables et morphologie du fond du lac du ...

    African Journals Online (AJOL)


    2Laboratoire de Géologie Marine et Sédimentologie ; UFR-Sciences de la Terre et des Ressources Minières, Université de Cocody,. 22 BP 582 ... Par ailleurs, cette étude a permis de réaliser la première carte bathymétrique du lac de Taabo 26 ans après sa mise en eau. ... de vie constitue une des conséquences les plus.

  1. Activation of arcuate nucleus neurons by systemic administration of leptin and growth hormone-releasing peptide-6 in normal and fasted rats. (United States)

    Luckman, S M; Rosenzweig, I; Dickson, S L


    Both leptin and growth hormone secretagogues are believed to have stimulatory effects on the hypothalamic growth hormone pulse generator, though whether these are achieved through the same pathway is unknown. Systemic administration of a normally maximal effective dose of the growth hormone secretagogue GHRP-6 to male rats causes the induction of c-Fos protein in the ventromedial aspect of the hypothalamic arcuate nucleus. The effect of the same dose of GHRP-6 is, however, much greater in animals that have been fasted for 48 h, suggesting that in the food-replete rat, arcuate neurons either show reduced sensitivity to endogenous growth hormone secretagogues or they are under the tonic inhibitory influences of other factors. The major populations of arcuate neurons activated by GHRP-6 have been shown to contain neuropeptide Y or growth hormone-releasing factor, while leptin is thought to be inhibitory to neuropeptide Y neurons. Leptin did not alter the response of the rats to GHRP-6. However, it was able by itself to induce c-Fos protein immunoreactivity in the ventral, including the ventrolateral, arcuate nucleus of fasted rats. This is a clear demonstration of the acute activation of arcuate neurons in the rat following systemic leptin injection and suggests that GHRP-6 and leptin act on the growth hormone axis via different pathways.

  2. Optimisation d'un systeme d'antigivrage a air chaud pour aile d'avion basee sur la methode du krigeage dual (United States)

    Hannat, Ridha

    The aim of this thesis is to apply a new methodology of optimization based on the dual kriging method to a hot air anti-icing system for airplanes wings. The anti-icing system consists of a piccolo tube placed along the span of the wing, in the leading edge area. The hot air is injected through small nozzles and impact on the inner wall of the wing. The objective function targeted by the optimization is the effectiveness of the heat transfer of the anti-icing system. This heat transfer effectiveness is regarded as being the ratio of the wing inner wall heat flux and the sum of all the nozzles heat flows of the anti-icing system. The methodology adopted to optimize an anti-icing system consists of three steps. The first step is to build a database according to the Box-Behnken design of experiment. The objective function is then modeled by the dual kriging method and finally the SQP optimization method is applied. One of the advantages of the dual kriging is that the model passes exactly through all measurement points, but it can also take into account the numerical errors and deviates from these points. Moreover, the kriged model can be updated at each new numerical simulation. These features of the dual kriging seem to give a good tool to build the response surfaces necessary for the anti-icing system optimization. The first chapter presents a literature review and the optimization problem related to the antiicing system. Chapters two, three and four present the three articles submitted. Chapter two is devoted to the validation of CFD codes used to perform the numerical simulations of an anti-icing system and to compute the conjugate heat transfer (CHT). The CHT is calculated by taking into account the external flow around the airfoil, the internal flow in the anti-icing system, and the conduction in the wing. The heat transfer coefficient at the external skin of the airfoil is almost the same if the external flow is taken into account or no. Therefore, only the

  3. Dynamique des populations du foreur des tiges du cacaoyer ...

    African Journals Online (AJOL)

    Les résultats obtenus ont montré qu'il y a deux périodes de fortes attaques de ce déprédateur au cours de l'année dans la région du Haut-Sassandra. Ces périodes se situent de Janvier à Avril avec un pic en Février et de Juin à Novembre avec un pic en Août. Pendant ces périodes, la population des larves est très élevée ...

  4. Du système de charges aux statuts de prestige individuel From the System of Charges to Individual Prestige Statuses: Thoughts on the Boundaries Between Power Spaces in Aguacatenango

    Directory of Open Access Journals (Sweden)

    Marie Chosson


    Full Text Available Les habitants du village tseltal d’Aguacatenango se trouvent confrontés aujourd’hui à différents bouleversements, dont les plus importants sont la prochaine réorganisation administrative et politique et, depuis une vingtaine d’années, la diversification des référents religieux. Ces transformations les obligent à adapter ou à reformuler ce qui pour eux constitue « la tradition » et, par là même, leur identité. L’une de leur principale préoccupation concernant ces changements est la mise en danger de leur conception de la bonne administration des affaires publiques. Les questions de distribution des pouvoirs sont ainsi au cœur des discours sur les processus actuels de « modernisation » de la communauté. Cet article se propose de revenir sur leur approche particulière de la notion de pouvoir par l’analyse de la forme d’organisation actuelle de leur administration, le système de charges. L’outil conceptuel de « frontières épaisses » permet de faire apparaître, à la marge du système, d’autres espaces de pouvoir, moins visibles mais tout aussi effectifs pour la population. Cette réflexion nous donnera une vision plus générale des différentes sphères de pouvoir et de leurs frontières, permettant pour la suite une lecture plus simple des véritables enjeux des changements actuels et des possibles stratégies d’adaptation qui en découleront.The inhabitants of the Tzeltal village of Aguacatenango currently find themselves confronted with various upheavals, the most serious of which are the upcoming administrative and political reorganisation and, over the past twenty years, the diversification of religious tenets. These transformations force them to adapt or reformulate what for them constitutes “tradition” and, by extension, their identity. One of their main concerns in relation to these changes is the endangerment of their conception of good public affairs administration. Therefore, power

  5. Le vide univers du tout et du rien

    CERN Document Server

    Diner, Simon


    Pourquoi l'Univers plutôt que le vide ? Le temps et l'espace existent-ils en l'absence de l'Univers ? Que reste-t-il quand tout est enlevé ? Pourquoi quelque chose plutôt que rien ? Depuis des siècles, ces interrogations mobilisent philosophes et physiciens. Mais aujourd'hui, le vide n'est pas le rien. Il serait même l'acteur central de l'histoire de la matière et de l'Univers, le partenaire privilégié de la physique. Vide et matière ne sont plus deux manifestations séparées de la nature, mais deux aspects d'une même réalité. Le vide est l'état de base dont la matière émerge, sans couper son cordon ombilical Le vide comme Univers du rien cède la place au vide comme Univers du tout. Que le vide puisse être conçu par les physiciens comme réservoir potentiel d'univers, voici qui ne devrait laisser personne indifférent. Ce livre ouvre un débat et nous convie à une réflexion surprenante.

  6. A geographic information system for the study of past epidemics: The 1705 epidemic in Martigues (Bouches-du-Rhône, France

    Directory of Open Access Journals (Sweden)

    Stéfan Tzortzis


    Full Text Available At the beginning of the 18th century, the Provence region was hit by several severe epidemics whose causes are still not clearly understood.To draw up epidemic profiles and to identify the pathogenic agents concerned, we constituted a large onomastic database and built ageographic information system for Martigues, a medium-sized community in the south of France. The cross-linking of epidemiological,spatial and demographical data allows us to propose a new diagnosis for the epidemic which reached Martigues in the autumn of 1705.

  7. les cahiers du cread: Submissions

    African Journals Online (AJOL)

    Le nom de l'auteur (en majuscule Times New Roman", gras, taille de 12), précédé de son prénom (en minuscule), se met 2 interlignes en dessous du titre, justifié à droite et suivi d'un astérisque renvoyant en bas de page où est mentionnée le nom de l'institution dont relève l'auteur (Centre ou unité de recherche, université) ...

  8. Har du rådne kunder?

    DEFF Research Database (Denmark)

    Grünbaum, Niels Nolsøe


    Tætte kunderelationer er ikke altid guld værd. Har du modet til at skille dig af med kunder, som ødelægger din forretning?......Tætte kunderelationer er ikke altid guld værd. Har du modet til at skille dig af med kunder, som ødelægger din forretning?...

  9. The case of Cirque du Soleil Dralion

    African Journals Online (AJOL)

    attendance from an individual motivation perspective is nearly non-existent”. This is especially true in the ... Further insights into visitors' motives can benefit Cirque du Soleil marketing with regard to market segmentation, ...... 'Cirque du Soleil extends balancing act to Asian cities', The Gazette, 17. May. Lamme, E. 2011.

  10. Report about the optimization of the biofuel industry sustaining system; Rapport sur l'optimisation du dispositif de soutien a la filiere biocarburants

    Energy Technology Data Exchange (ETDEWEB)

    Prevot, H.; Hespel, V.; Dupre, J.Y.; Baratin, F.; Gagey, D


    At the end of 2004, the French government has fixed up the ambitious goal of developing biofuels conformably with the objectives of the 2003/30/CE European directive: the level of blending gasoline and diesel fuels with biofuels should reach 5.75% of the energetic value by 2010. In 2004 this level was only 0.8%, i.e. 7 times less. In order to reach such a goal, the government has implemented two tools: a classical tax exemption tool, already used by other European partners, and a new tool created by the 2005 finances law: the general tax on polluting activities (TGAP). This report presents the main characteristics of biofuel industries and the policies implemented in favor of biofuels. It analyzes the new system and its implementation (tax exemption and TGAP) and proposes new markets for the French agriculture. It recommends to take into considerations the constraints and needs of the fuels market, that the government establishes a new regulation for this market, reforms the existing fiscal system and takes complementary dispositions (intervention at the European Communities level, development of research..). Several appendixes illustrate this report. (J.S.)

  11. Patrice Blouin/Images du sport


    Huitorel, Jean-Marc


    « Notre but, en effet, dans les pages qui vont suivre, est d’écrire une histoire du sport moderne par le biais de sa mise en images » (p. 18).  Enoncé en début de texte, le projet est alléchant et ambitieux. En fait, Images du sport pourrait aussi bien s’intituler Prolégomènes pour une histoire du sport, la thèse qui sous-tend l’entreprise étant que ladite histoire du sport se confond largement avec l’histoire de ses représentations et plus particulièrement, c’est du moins l’angle que privilé...

  12. Replacing an electrical heating system by an air/water heat pump; Remplacement du chauffage electrique par une installation PAC air/eau (pompe a chaleur)

    Energy Technology Data Exchange (ETDEWEB)



    A group of thirteen houses in Plan-les-Ouates (southwestern Switzerland) constructed in 1985 was originally equipped with direct electric heaters. Their inhabitants complained about high electricity bills and bad thermal comfort due to uneven room temperatures and insufficient amounts of warm water. A group of specialists from the Swiss Federal Office of Energy, the energy service of the canton of Geneva and from an engineering company studied the case and suggested to replace the direct electric heating system by an air to water heat pump. Seven owners have decided for this modification and the retrofit has been made in autumn 2000. The paper presents the results of performance measurements, operating experience gained and economic considerations for the years 2001, 2002 and 2003 for six installations. The average coefficients of performance ranged from 2.3 to 2.9.

  13. Infection tracers: past, present and future; Radiotraceurs et recherche d'infection du systeme musculo-squelettique: passe, present et futur

    Energy Technology Data Exchange (ETDEWEB)

    Turpin MD, S. [Centre Hospitalier Universitaire, Hotel Dieu de Montreal, Dept. de Medecine Nucleaire, Quebec (Canada)


    Clinical and para-clinical findings are still the mainstay for the diagnosis of musculoskeletal infections, especially osteomyelitis. No single complementary imaging technique has 100% specificity and sensitivity for every case of musculoskeletal infection. Depending on the age of the patient, presence of orthopedic hardware, location of infection, underlying bone and systemic conditions, the choice of imaging modalities must be tailored to the patient's condition. Plain radiographs are performed first and may be sufficient. In children, bone scan is highly accurate to diagnose osteomyelitis. Labeled leukocytes with complementary bone or bone marrow studies are recommended for orthopedic hardware or diabetic foot. Finally, gallium scanning is useful for the diagnosis of vertebral osteomyelitis. Current radiopharmaceuticals used for diagnosing infection also label inflammation, which limits specificity. Newer products, as Infecton and {sup 18}F-FDG, are currently under investigation for the differentiation between infection and sterile inflammation. (author)

  14. Rejets et environnement du CERN

    CERN Document Server

    Vojtyla, P


    La radioactivité de l’environnement autour de l’Organisation Européenne pour la Recherche Nucléaire (CERN) et les doses de rayonnements qui en résultent pour la population avoisinante sont contrôlées par la Commission de Sécurité du CERN et de manière indépendante par les autorités compétentes des deux États Hôtes, l’Institut de Radioprotection et de Sûreté Nucléaire (IRSN) côté France et l’Office Fédéral de la Santé Publique (OFSP) côté Suisse (voire Chapitre 8.6). Dans ce rapport, les résultats de mesures concernent en particulier le territoire suisse. L’ensemble des mesures effectuées en 2006 prouve que le fonctionnement des installations du CERN était sans conséquence radiologique sur l’environnement et la population. Le contrôle des émissions montre que les rejets effectifs se situent, également en 2006, nettement en dessous des limites réglementaires. Ce constat est confirmé par le contrôle des immissions dans l’environnement. Le CERN a bien respecté en 2...

  15. Sociologie et sociolinguistique du franbreu

    Directory of Open Access Journals (Sweden)

    Ben-Rafael Eliezer


    Full Text Available Des enquêtes ont montré que plus de 20 % des adultes israéliens ont quelque connaissance du français. Parmi cette population, 250 000 à 300 000 personnes pouvaient être considérées comme francophones. Le français a affirmé une présence importante – pas toujours reconnue – dès la phase cruciale du développement de cette société. Une immigration de masse suivit la création de l’État (1948 dont la population a quasiment triplé en moins de deux décennies, avec l’arrivée de plus de 200 000 immigrants d’Afrique du Nord et des dizaines de milliers des Balkans et du Moyen-Orient. Beaucoup parmi ces immigrants étaient francophones – le français étant pour eux soit la première langue soit une deuxième qu’ils contrôlaient. C’est depuis lors que la présence du français est devenue démographiquement significative dans la société israélienne, se prolongeant de nos jours dans l’immigration en provenance de France qui se poursuit jusqu’à aujourd’hui. Comme nous l’avons montré ailleurs (Ben-Rafael et Ben-Rafael 2013, Israël fait l’expérience simultanée de plusieurs francophonies originales qui diffèrent par leurs sources aussi bien que leurs expressions (Ben-Rafael et Ben-Rafael 2013. Un premier modèle est le « français vernaculaire » (voir Boudras-Chapon 2008 amené par les immigrants de classes défavorisées arrivant d’Afrique du nord qui se sont regroupés en Israël. Leur français n’a offert qu’une faible résistance à l’hébreu qui s’est imposée dans le processus d’intégration. Un bilinguisme fonctionnant sur un mode additif au début qui s’est mué progressivement en modèle soustractif. Un second modèle concerne les immigrants de même origine ainsi que des immigrants francophones d’autres pays (pays balkaniques, Turquie ou Egypte formant une strate instruite de classe moyenne, pour qui le français était d’utilisation quotidienne aussi bien que de langue de

  16. Bases en technique du vide

    CERN Document Server

    Rommel, Guy


    Cette seconde édition, 20 ans après la première, devrait continuer à aider les techniciens pour la réalisation de leur système de vide. La technologie du vide est utilisée, à présent, dans de nombreux domaines très différents les uns des autres et avec des matériels très fiables. Or, elle est souvent bien peu étudiée, de plus, c'est une discipline où le savoir-faire prend tout son sens. Malheureusement la transmission par des ingénieurs et techniciens expérimentés ne se fait plus ou trop rapidement. La technologie du vide fait appel à la physique, à la chimie, à la mécanique, à la métallurgie, au dessin industriel, à l'électronique, à la thermique, etc. Cette discipline demande donc de maîtriser des techniques de domaines très divers, et ce n'est pas chose facile. Chaque installation est en soi un cas particulier avec ses besoins, sa façon de traiter les matériaux et celle d'utiliser les matériels. Les systèmes de vide sont parfois copiés d'un laboratoire à un autre et le...

  17. Analyse de la formation des phases du systeme cuivre-germanium par diffraction des rayons X sur des echantillons d'epaisseur nanoscopique (United States)

    Aubin, Alexandre

    With the miniaturization of electronic devices, driven by cost reduction and performance increase, new materials have to be introduced in their fabrication process to solve many emerging problems. These challenges are brought forth by the ITRS (International Technology Roadmap for Semiconductors), a comity in charge of listing the technological needs for the upcoming generations of integrated circuits. Many fields of interest require new technological developments, from global to local interconnections, the transistor gate, the gate insulator thickness, etc. One of the major challenges mentioned in the ITRS roadmap is the need for a new interconnection material. Indeed, the need for a diffusion barrier for the copper lines in local and global interconnections of integrated circuits, the main technology in today's devices, is becoming more hindering with the decrease of the metallization lines. cross-section. In the 90's, a binary compound of copper and germanium, known as the epsilon1-Cu3Ge phase, was investigated as a replacement for aluminum because of its low resistivity, that can reach as low as 5.5muO·cm [1], its stability in contact with both silicon and silicon oxide [2] as well as its thermal stability during anneals [3]. However, copper proved to be a better choice at the time because of its low bulk resistivity of 1.68muO·cm at room temperature[4]. The objective of this master thesis is to re-examine the copper-germanium system, and more specifically the epsilon1-Cu3Ge, for future applications in the microelectronics industry. Different X ray diffraction techniques were used to obtain more information on the system, including in situ X ray diffraction during 3°C/s anneals in an inert helium atmosphere with simultaneous resistance measurement, theta-2theta scans to detect diffraction peaks of the present phases after sample quenching as well as partial acquisition of the reciprocal space of quenched samples which allowed to obtain pole figures for d

  18. De Sumer à Seattle, le voyage du bout du monde

    Directory of Open Access Journals (Sweden)

    Olivier Walther


    Full Text Available « Les affaires qui se nouent par-dessus les frontières qui séparent les peuples ne font pas que ces frontières n'existent pas » notait Émile Durkheim en 1895 (1895 : 113. Un peu plus d’un siècle plus tard, l’ouvrage de William J. Bernstein intitulé A Splendid Exchange et sous-titré de manière inspirée How Trade Shaped the World vient apporter une confirmation étayée au sociologue français. De Sumer où fut introduit le commerce du cuivre 3000 ans avant Jésus-Christ, à Seattle où se tint la tr...

  19. Prise en charge communautaire du paludisme chez Ia femme ...

    African Journals Online (AJOL)

    Avant la mise en place d'une intervention visant a tester 1' efficacite du diagnostic et du traitement systematique du paludisme chez la femme enceinte au Benin, au Burkina Faso et en Gambie, une revue systematique de la litterature relative a la prise en charge du paludisme a base communautaire a ete realisee dans ...

  20. Nouvelles du Centre Aéré de l’Association du Personnel du CERN

    CERN Multimedia

    Jardin d'enfants


    Cet été 2015, durant quatre semaines d’été, le Centre Aéré a accueilli plus de 40 enfants âgés de 4 à 6 ans. Devant le succès rencontré, et à la demande des parents, il a été décidé d’en doubler la capacité maximale. A l'été 2016, du 4 au 29 juillet, la structure pourra accueillir les enfants de 4 ans révolus et de moins de 7 ans (nés après le 31/07/2009 mais avant  01/07/2012). Les inscriptions se feront à la semaine durant le mois d'Avril 2016. Les programmes sont en cours d'élaboration cependant nous pouvons déjà vous communiquer le thème conducteur du centre qui sera : à la découverte d'un continent différent chaque ...


    CERN Document Server


    La Direction du Crédit Agricole informe son aimable clientèle du CERN des jours et heures d'ouverture de l'agence du site de Prévessin à compter du mardi 14 janvier 2003 : 1. Horaires pour les opérations bancaires courantes 7 jours sur 7 et 24h/24 avec l'espace libre service bancaire. 2. Horaires conseil du mardi au vendredi - Mardi, de 9h. à 12h. et de 14h.15 à 16h.30. - Mercredi, jeudi et vendredi, de 9h. à 12h. et de 13h.30 à 16h.30. Deux collaboratrices au lieu d'une seront désormais présentes toute la journée du mardi au vendredi pour vous accueillir, vous informer et vous conseiller en crédits et placements (réception conseil sur rendez-vous). Autre nouveauté : les mêmes conseillers seront aussi à votre disposition le samedi, sur notre agence de Gex, de 8h.15 à 13h.05, notamment pour les études de financements habitat. La Direction et toute l'équipe de l'agence du Crédit Agricole vous souhaitent une excellent année 2003.

  2. Construire l’esprit du dauphin


    Servais, Véronique


    Est comparée ici la manière dont l’« esprit » du dauphin est construit dans six contextes interactionnels distingués par le degré de participation (au sens ethnographique du terme) qu’ils impliquent de la part des êtres humains, et éventuellement des dauphins : l’étude du cerveau, l’étude des capacités sensorielles, les recherches sur la cognition, l’observation naturaliste, le discours des delphinariums et la rencontre en pleine mer. En vertu de ces comparaisons, il est avancé que l’attribut...

  3. Flaubert et Du Camp : quelques remarques

    Directory of Open Access Journals (Sweden)

    Michel Brix


    Full Text Available The Flaubert scholars accuse Maxime Du Camp of several instances of dishonesty and don’t pay attention to what Du Camp has said or written. This regrettable situation deprives the readers of numerous pieces of information which are likely to shed light on the stakes but also on the contradictions of the flaubertian poetics. This article takes a look at a few revealing cases and especially looks into the similitaries between a passage of the Du Camp’s book Le Nil (1854 and the episode where Marie Arnoux makes her appearance, in L’Éducation sentimentale (1869.

  4. Harald Weinrich, Linguistique du mensonge


    Torterat, Frédéric


    Dans les sociétés contemporaines, où la traque à la désinformation est autant l’apanage des médias que des initiatives citoyennes et de la communication des partis politiques, les mécanismes discursifs du mensonge font l’objet d’une veille partagée. On pourrait presque parler, avec un brin de provocation, d’un réseau éparpillé de guetteurs, parmi lesquels les lanceurs d’alerte constituent les personnages les plus emblématiques. Les alertes sur le double langage et sur les contrevérités n’ont ...

  5. Rejets et environnement du CERN


    Vojtyla, P; Wittekind, D


    La radioactivité de l’environnement autour de l’Organisation Européenne pour la Recherche Nucléaire (CERN) et les doses de rayonnements qui en résultent pour la population avoisinante sont contrôlées par la Commission de Sécurité du CERN et de manière indépendante par les autorités compétentes des deux États Hôtes, l’Institut de Radioprotection et de Sûreté Nucléaire (IRSN) côté France et l’Office Fédéral de la Santé Publique (OFSP) côté Suisse (voire Chapitre 8.6). Dans ce rapport, les résul...

  6. [About the Baume du commandeur]. (United States)

    Labrude, Pierre


    The Baume du commandeur appears in Franch in 1694 in the Histoire générale des drogues [...] written by Pomet. It's a vulnerary drug with also numerous internal uses. Still present in formularies and sometimes used, its invention is attributed to Gaspard de Pernes, commander of Malta Order in Toul, in France. However, some observations induce us to think that the origin of this drug would be in eastern countries: first one of its other names, baume persique, secondly the name of a comparable other formula baume des Turcs, then the geographical origin of the majority of plants and drugs constituting the initially published formula, finally the appurtenance of G. de Pernes to Malta Order. Unfortunately, the transmissions, modifications and improvements of the formula since its beginning make today very difficult to discover the localization and dating of the creation of this famous drug.

  7. Le Carrousel du temps : l'aventure du LHC est un voyage à travers le temps ....

    CERN Multimedia

    Jean Leyder


    Les découvertes archéologiques sur l'un des sites du LHC nous ramènent quelques 1700 ans en arrière. Les ingénieurs ont creusé la roche d'environ 208 à 144 millions d'années, et avec les collisions du LHC, le voyage aboutira à l'origine du temps lui même.

  8. Métastases gastro-intestinales du cancer du sein: à propos de 2 cas ...

    African Journals Online (AJOL)

    Le cancer du sein est le cancer le plus fréquent chez la femme, notamment au Maroc, avec un taux de mortalité élevé. Les métastases gastro- intestinales d'un carcinome canalaire du sein sont rares. Leur diagnostic est difficile du fait de la nature non spécifique des symptômes. Nous rapportons deux observations ...

  9. Visite du Conseil des gouverneurs du CRDI en Afrique de l'Est ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    17 oct. 2017 ... En juillet dernier, des membres du Conseil des gouverneurs du CRDI se sont rendus en Afrique de l'Est pour rencontrer des bénéficiaires de subventions de recherche du Centre au Kenya, en Ouganda et en Tanzanie. Cette visite d'une durée d'une semaine, organisée par le Bureau régional de l'Afrique ...

  10. Sulphite metabolism; Metabolisme du sulfite

    Energy Technology Data Exchange (ETDEWEB)

    Fromageot, P.; Chapeville, F. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires


    Although the formation of sulphite by micro-organisms was observed as early as 1914 by Neuberg, it was only in 1932 that Nightingale showed it to be present inside cells, in the case of tomato leaf stalks, its formation being due to the reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. The presence of the thiosulphate formed was shown by the high refractive index of its barium salt. Today we have a certain amount of data concerning the formation and use of sulphite in the living cell, even through the knowledge is still incomplete. In this article we will describe and discuss the facts which are known, with particular reference to the oxidation of sulphite. (author) [French] Si la formation de sulfite par des micro-organismes a ete observee des 1914 par Neuberg, sa presence a l'interieur des cellules a ete demontree pour la premiere fois par Nightingale en 1932, dans des tiges et des feuilles de tomates, par la reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. Le thiosulfate forme etait mis en evidence par l'indice de refraction eleve de son sel de baryum. Aujourd'hui on possede un certain nombre de donnees, cependant encore tres incompletes, sur la formation du sulfite et son utilisation par la cellule vivante. Dans cet expose nous decrirons et discuterons quelques-unes de ces acquisitions, tout particulierement celles relatives a l'oxydation du sulfite. (auteur)

  11. Besoins et pratiques du dosage de l'eau du procédé d'extraction du ...

    African Journals Online (AJOL)

    , la fréquence d'ajout d'eau et les besoins en eau du procédé de barattage. Les résultats montrent que la température de l'eau et la fréquence d'ajout d'eau influencent la durée et les besoins en eau du process de barattage. Les besoins en ...

  12. 69 Optimisation du procédé de fabrication du tchapalo, bière ...

    African Journals Online (AJOL)


    Le plan d'expérience, précisément le plan factoriel fractionné a été appliqué sur la méthode de fabrication artisanale du tchapalo pour l'amélioration du procédé. Ainsi, après définition du domaine expérimentale, le criblage des facteurs a mis en exergue l'importance de la phase du brassage, notamment les étapes de.

  13. Daphne du Maurier' unustatud jutud / Udo Uibo

    Index Scriptorium Estoniae

    Uibo, Udo, 1956-


    Ajalehe "The Independent" teatel kavatseb kirjastus "Virago" maikuus avaldada Daphne Du Maurier lühiproosa kogumiku "Nukk" (The Doll), mille on koostanud tema loomingu suur austaja Ann Willmore ja mis sisaldab 13 unustatud ja seni avaldamata novelli

  14. Inauguration du C/R Forum (Belgique

    Directory of Open Access Journals (Sweden)


    Full Text Available Le 16 février dernier, la Vlaamse beroepsvereniging Conservatie en Restauratie Forum (C/R Forum a été constituée à l'initiative de 18 personnes du milieu de la conservation/restauration et du secteur de la protection du patrimoine. Etant donné que la conservation et la gestion du patrimoine culturel mobilier et immobilier de même que la formation et la recherche relèvent des communautés et des régions, la nécessité est apparue depuis un certain temps de créer une organisation Flamande au sei...

  15. Conversion chimique du gaz naturel Chemical Conversion of Natural Gas

    Directory of Open Access Journals (Sweden)

    Chaumette P.


    Full Text Available Dans cet article sont passés en revue les travaux de recherche et développement et les procédés existants dans le domaine de la conversion chimique du gaz naturel. Les deux voies possibles, conversion directe du méthane et conversion indirecte, via le gaz de synthèse, sont présentées. Tant la préparation d'hydrocarbures utilisables comme carburants, que celle des composés de bases pour la pétrochimie ou la chimie sont évoquées. L'accent est mis sur l'étape clé du développement de chaque procédé qui, selon le produit visé, consiste en la mise au point d'un nouveau système catalytique, en un changement de la technologie du réacteur, ou en la mise au point d'une section fractionnement moins complexe. This article reviews the research and development work and the existing processes in the area of chemical conversion of natural gas. The two possible methods, direct conversion of methane and indirect conversion via synthesis gas, are discussed. The preparation of hydrocarbons that can be used as fuels and the production of building blocks for the petrochemical and chemical industries are both dealt with. The accent is placed on the key step in developing each process. Depending on the target product, this key step consists in working out a new catalytic system, changing reactor technology or engineering a less complex fractionation section.

  16. Aux frontières du politique et du religieux

    Directory of Open Access Journals (Sweden)

    Frédéric Sylvanise


    Full Text Available The Autobiography of Miss Jane Pittman met en scène de manière spectaculaire deux figures de leadership noir-américain au XIXe siècle (Ned et au XXe siècle (Jimmy. Ned, fils adoptif de Jane Pittman, se rebaptise Professor Douglass en hommage au leader abolitionniste Frederick Douglass. S’il est fondamentalement laïc (il est instituteur, le texte lui confère néanmoins une dimension religieuse, notamment dans le chapitre intitulé « The Sermon at the River » , qui renvoie à la fois à Moïse (le fleuve et à Jésus (le sermon sur la montagne. Abattu par un tueur à gages, Ned est un martyr dont l’histoire était déjà écrite. Le personnage de Jimmy, attendu par toute une communauté comme le messie (alors que lui aussi est un laïc et un homme qui perd la foi, s’inscrit dans la même histoire. Lettré, éloquent et impliqué dans le mouvement pour les droits civils, il est assassiné comme son prédécesseur. Dans les deux cas, le texte lie donc le politique et le biblique comme s’il ne faisait qu’un : être un leader politique, c’est être un preacher et un messie qui guide le peuple. Il est possible de lire l’évocation de ces deux hommes à la lumière d’une certaine actualité. Ned et Jimmy connaissent en effet un sort proche de celui de Malcolm X et de Martin Luther King, récemment assassinés au moment de l’écriture de The Autobiography of Miss Jane Pittman. C’est l’histoire de la répression du leadership noir, au travers du portrait de deux de ses avatars, qui peut donc se lire en creux ici.

  17. Caracterisation du rajeunissement du noyer (Juglans sp.) pour une etude spectrophotometrique globale du contenu polyphenolique


    Jay Allemand, Christian; Cornu, D.; Macheix, J.J.


    Cette étude a eu pour objet de rechercher des marqueurs biochimiques de la juvénilité chez le Noyer pour permettre d’apprécier l’état d’aptitude à la multiplication végétative des boutures et des microboutures avant et pendant l’application des traitements (température, lumière, milieux de culture...). A partir d’analyses spectrophotométriques effectuées sur des extraits purifiés de composés phénoliques de différentes catégories de pousses annuelles (selon l’âge du pied-mère, la taille, ...

  18. La fin du risque zéro : du homegrown jihadism au terrorisme du loup solitaire

    Directory of Open Access Journals (Sweden)

    Rémi Baudouï


    Full Text Available Le risque zéro représente un moment historique particulier dans la conceptualisation de la socio-politique du risque. Son application repose au début des années 1980 sur l’hypothèse de la possibilité de progresser dans le domaine de l’anticipation et de la gestion sécuritaire des crises potentielles au point de pouvoir prémunir les populations contre toute forme de menace. Il fut employé dans les domaines de la protection civile et de la sécurité militaire. Comme élément de gouvernance politique, il réfutait l’idée selon laquelle le risque existe en préalable comme catégorie d’acceptabilité collective de la menace.

  19. Report of the National Sub-Committee to the Interprovincial Standards Program Coordinating Committee on the Interprovincial Computerized Examination Management System=Rapport du Sous-Comite National presente au Comite de Coordination du Programme de Normes Interprovincials sur le systeme de gestion des examens informatises interprovinciaux. (United States)

    New Brunswick Dept. of Advanced Education and Training, Fredericton. Interprovincial Standards Program Coordinating Committee.

    In January 1985, Employment and Immigration Canada funded a pilot project in New Brunswick for the development and testing of an Interprovincial Computerized Examination Management (ICEM) System. The resulting system is comprised of a dual interprovincial and provincial item bank facility, a software component offering the option of computerized…


    African Journals Online (AJOL)

    cervico-vaginal au cours du suivi prénatal en pays pauvre très endetté. MOTS CLES: Prélèvement cervico-vaginal - Streptocoque â-hémolytique du groupe B - Transmission materno-fœtale - Rupture prématurée des membranes - Infection néonatale. GROUP B BETA HEMOLYTIC STREPTOCOCCUS IN PREGNANCY AND ...

  1. Les effets du changement climatique dans le bassin du Congo : la ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    22 avr. 2016 ... Dans le bassin du fleuve Congo, plus de 80 % des habitants vivent exclusivement de l'agriculture, de la pêche, de l'élevage et de la cueillette, qui sont des activités largement tributaires du climat.

  2. Effets du type de fertilisation sur la productivité du Jatropha curcas ...

    African Journals Online (AJOL)

    Effets du type de fertilisation sur la productivité du Jatropha curcas en zone des savanes d'Afrique Centrale cas de la localité de Sanguéré Paul au Nord Cameroun. Adamou Issa, Sali Bourou, Mana Pierre Hamadou, Patrick Prudent, Jean Leroy ...

  3. Incidence du Yam mosaic virus (YMV) et du Cucumber mosaic virus ...

    African Journals Online (AJOL)

    Incidence du Yam mosaic virus (YMV) et du Cucumber mosaic virus (CMV) sur des variétés de Dioscorea spp. cultivées dans les régions de Bouaké et de Toumodi en Côte d'Ivoire. K Seka, AH Diallo, NK Kouassi, S Ake ...

  4. Engagez-vous, devenez délégué(e) du personnel du CERN

    CERN Multimedia

    Staff Association


    Dans notre ECHO N° 275, nous avons annoncé les élections à venir au Conseil du personnel du CERN. Dans le présent ECHO, nous vous informons du lancement du processus des élections qui débute par le dépôt des candidatures. Tous les titulaires, boursiers et associés, qui sont aussi membres de l’Association du personnel, peuvent s’engager et déposer leur candidature entre le 11 septembre à 08 h 00 et le 13 octobre 2017 à 17 h 00. N’hésitez plus, remplissez le formulaire de candidature, présentez-vous aux élections au Conseil du personnel afin de pouvoir représenter et défendre vos collègues du personnel du CERN. ÊTRE DÉLÉGUÉ(E), C’EST QUOI ? Poser la question à plusieurs d...

  5. Influence de la provenance du géniteur et du type de pollen sur la ...

    African Journals Online (AJOL)

    Influence de la provenance du géniteur et du type de pollen sur la fructification sous pollinisation contrôlée chez Dacryodes edulis (Burseraceae) au Cameroun. JT Makueti, Z Tchoundjeu, A Kalinganire, BA Nkongmeneck, E Asaah, A Tsobeng, A Kouodiekong ...

  6. Synthesis of phenobarbital {sup 14}C - 5 (1964); Synthese du phenobarbital {sup 14}C - 5 (1964)

    Energy Technology Data Exchange (ETDEWEB)

    Benakis, A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Phenobarbital (Luminal), well known as a hypnotic agent, plays also an important role in the medical treatment of epilepsy. With the object of studying the metabolism of this medicament in the living system, the synthesis of phenobarbital marked in position 2 of the pyrimidinic ring has been effected. It has been necessary, in order to carry out to research into the metabolism of this product, to have a phenobarbital marked in position 5 of the pyrimidinic ring. This synthesis, making it possible to introduce C-14 into position 5 of the pyrimidinic ring, consists of seven stages starting from C-14 carboxyl benzoic acid having a specific activity of 25 mc/mM. The melting point of the final product is 172 - 173 deg. C and its specific activity is 11.15 mc/mM. The overall radioactive yield of the pure product, with respect to the original benzoic acid (10 mM) is of the order of 3 per cent. The purity of the product has been controlled by paper chromatography; it is of the order of 99 per cent. (author) [French] Le phenobarbital (Luminal) bien connu comme agent hypnotique, joue egalement un role important dans la medication anti-epileptique. Pour l'etude du metabolisme de ce medicament dans l'organisme vivant, nous avons synthetise le phenobarbital marque en position 2 du cycle pyrimidinique. Afin de poursuivre la recherche du metabolisme de ce produit, il a ete necessaire de disposer du phenobarbital marque en position 5 du cycle. pyrimidinique. Cette synthese, permettant l'introduction du {sup 14}C en position 5 du cycle pyrimidinique, comporte sept etapes, au depart de l'acide benzoique carboxyle {sup 14}C, d'une activite specifique de 25 mc/mM. Point de fusion du produit final: 172 - 173 deg. C Activite specifique: 11,15 mC/mM. Rendement total radioactif du produit pur, par rapport a l'acide benzoique de depart sur 10 mM, de l'ordre de 3 pour cent. La purete du produit a ete controlee par chromatographie sur papier; elle est de l

  7. Design and implementation of the decision unit of the first level trigger system of the LHCb detector at the Large Hadron Collider (LHC); Conception et realisation de l'unite de decision du systeme de declenchement de premier niveau du detecteur LHCb au LHC

    Energy Technology Data Exchange (ETDEWEB)

    Laubser, J


    The LHCb experiment is one of the four particle physic detector installed at the new Large Hadron Collider (LHC) at CERN in Geneva. In order to reduce the amount of data storage for offline analysis, an online trigger system of interesting event according to the studied physic is implemented in parallel of the Data Acquisition system. The trigger system is composed by a first level (Level-0) made by a complex electronic system and a second level made by a computing system called the High Level Trigger. The Level-0 Decision Unit is the central part of the first trigger level that takes the decision to accept or to reject the event by using a fraction of information coming from the fastest sub-triggers (432 bits at 80 MHz). It is a full custom 16 layers board using advanced FPGA (Field Programmable Gate Array) in BGA (Bill Grid Array) package. Each sub-trigger transmit their data via high speed optical links running at 1.6 Gbit/s. The processing is implemented using a 40 MHz synchronous pipelined architecture. It performs a simple physical algorithm to compute the Level-0 trigger decision in order to reduce the data flow from 40 MHz down to 1 MHz for the next trigger level. The internal design of the processing FPGA is mainly composed by a Partial Data Processing (PDP) and a Trigger Definition Unit (TDU). The aim of the PDP is to adjust the clock phase, perform the time alignment, prepare the data for the TDU and monitor the data processing. The TDU is flexible and allows to fully re-configure all the trigger conditions through the Experiment Control System without any FPGA re-programming. (author)

  8. L'intermittence, nouvelle norme du marché du travail ?


    Pilmis, Olivier


    Si le développement de l’emploi à durée déterminée ou à temps partiel constitue l’une des évolutions majeures du marché du travail au cours des dernières décennies, faire de l’intermittence une norme sur le marché du travail pose problème. En effet, c’est oublier que le marché du travail s’organise, en France, autour du contrat à durée indéterminée à temps plein. Les discours appelant de leurs vœux l’avènement d’un travail libéré omettent, quant à eux, que les formes particulières d’emploi se...

  9. Scientific works of research workers of the army health service; Travaux scientifiques des chercheurs du service de sante des armees

    Energy Technology Data Exchange (ETDEWEB)



    Ten articles about the effects of gamma radiation on human or animal cells are studied here. Regulation of cytokines, effect of interleukin 6, expression of the C-fos protein, interest of the study of a biological check up after an accidental irradiation, interaction and transport of cysteamine( radioprotector), approach of a treatment of radioinduced medullary aplasia, expression of TNF alpha and gelatinase B, are the subjects related here. (N.C.).

  10. Aux marges du monde arabe

    Directory of Open Access Journals (Sweden)

    Hélène Thiollet


    Full Text Available L’étude des migrations contemporaines des Érythréens vers le Yémen au tournant des années quatre-vingt-dix permet d’observer les transformations des dynamiques régionales à l’œuvre dans le monde arabe. Les migrations sont un phénomène sensible aux différents aspects (politiques, économiques, culturels, géographiques de l’intégration régionale. Celle si est envisagée dans cet article à travers une approche doublement marginale : -la marginalité géographique de l’Érythrée arabo-africaine et du Yémen, économi­quement isolé dans la péninsule Arabique, -l’étude des migrations formelles et informelles, élément souvent marginal dans l’étude des institutions et des échanges qui constituent un système régional intégré. Cette étude est fondée sur une série d’entretiens menés au Yémen entre février et avril 2002 auprès des administrations yéménites et de la population érythréenne.


    Directory of Open Access Journals (Sweden)

    Marc Smeets


    Full Text Available Le voyage en Hollande, au XIXe siècle, s'inscrit dans une longue tradition où résonnent les notions de liberté, tolérance et commerce, mais il acquiert aussi une spécificité étant donné l’importance que prend la figure du « chez soi » : les Pays-Bas, terre d'agrément où le voyageur français se sent à l'aise et où il aimerait, si possible, vivre. Proust, de ce point de vue-là, ne fait rien de neuf quand il rêve dans la Recherche d'une « vie domestique » en terre batave. Les Pays-Bas, pour le voyageur français au XIXe, c'est le home sweet home.

  12. Evaluation du systeme de traduction automatique SYSTRAN anglais-Francais, version 1978, de la Commission des Communautes Europeennes (Evaluation of the English-French SYSTRAN Machine Translation System, 19 version, of the Commission of European Communities). (United States)

    Van Slype, Georges


    Describes the French-English version of the SYSTRAN machine translation system, used by the Commission of European Communities, and an evaluation study which examined the quality, cost, and weaknesses of the system. The study concluded that this type of system is useful for "pretranslation" and "rough" translation only. (AM)

  13. La Base de Donnees ERIC: Evaluation de Son Utilisation et Discussion des Choix du Systeme (The ERIC Database: An Evaluation of Its Use and a Discussion of the System Model). (United States)

    Malrieu, Denise


    This overview of the ERIC system begins with a brief history of the system; a description of the types and numbers of materials contained in the database; sources of types of information for educators that are not processed by ERIC; and the various publications and reference materials produced by and for the system. The analysis of ERIC usage in…

  14. Il mercato del lavoro nel mutamento del sistema penitenziario: dalle rivoluzioni industriali ai networks sociali / Labour market and changement of the penitentiary system: from the Industrial Revolutions to the social networks / Marché du travail et changement du système pénitentiaire à partir des révolutions industrielles jusqu'aux réseaux sociaux

    Directory of Open Access Journals (Sweden)

    Fanci Giovanna


    Full Text Available There is a close connection between the prison institution and the labour market which leads back to the origins of prison, in a modern sense. The social, economic and political background of this relationship is marked by the “industrial revolutions”, because during these times the ideas of «division of labour» and «discipline» were defined and consequently influenced the new prison organization models. The Penitentiary Act recognizes the prisoner's right to work. Therefore to fill the gap between the prison and the territory, a political integrated model – both social and penitentiary – is required. In the text, this will be illustrated by experiences of prisoners and ex-prisoners at the Information Office Windows. The network method has given input to a procedure considered as an efficient way of managing the social complexity and a fair services distribution device; but, at the same time, it introduces “alternative” forms of social control.La naissance des prisons et le marché du travail sont liés par une relation qui revient aux origines de la prison en sens moderne. La scène qui fait d’arrière-plan à cette relation est contremarquée par les révolutions industrielles pendant lesquelles les notions de «division du travail» et de «discipline» ont été définies et ont étés décisives afin d'élaborer un modèle nouveau d'organisation des prisons. Aujourd’hui la législation pénitentiaire reconnaît au prisonnier le droit au travail: de cette façon, la liason entre la prison et le territoire requiert un plan intégré composé par de politiques pénitentiaires et sociales, ici expliqué par l’expérience positive des Guichets d'information pour détenus et ancien détenus. Le système de réseau a fait démarrer une “proceduralisation” qui apparaît un outil efficace de gestion de la complexité sociale et d’une equitable destination des services, mais qui risque de produire aussi des systèmes

  15. Increase of gas transportation reliability as a foundation of dispatching management and training integrated systems; Accroissement de la fiabilite de transport du gaz a la base des systemes integres de la gestion dispatching et de la formation

    Energy Technology Data Exchange (ETDEWEB)

    Grigoriev, L.I.; Posyagin, B.S. [S.A. Sardanashvili (Russian Federation)


    In order to ensure the reliability and safety of modern complex gas transportation systems, specific simulators have been developed for the training of dispatching centers personnel. This article presents Gazprom's Russian experience in the development of computerized integrated management systems for the monitoring of gas pipeline networks. These systems integrate data collection, storage, processing, visualization and modeling tools for decision making, real-time management, tests and training. (J.S.)

  16. Établissements recevant du public

    CERN Document Server


    Synthèse pour les installations électriques, des règles de sécurité contre les risques d’incendie et de panique dans les établissements recevant du public (ERP), selon l’arrêté du 25 juin 1980 modifié. L'ouvrage traite à la fois des dispositions communes aux établissements du 1er groupe (1ère à 4ème catégorie), du 2ème groupe (5ème catégorie) et des règles particulières applicables aux différents types d'établissements (structures d'accueil pour personnes âgées ou handicapées, hôtels, magasins de vente, restaurants et débit de boisson, établissements de soins, administration, châpiteaux, etc...) Sont ainsi notamment traités, dans le cadre des dispositions communes aux établissements du 1er groupe, pour les installations électriques normales : les installations des appareils, tableaux et canalisations, les locaux électriques et installation de machines, l'éclairage normal des locaux accessibles au public, le chauffage et la ventilation, l'installation d'eau chaude sanita...

  17. Use of Take-set system for radiopharmaceutical labelling: example of indium-111 labeled pentetreotide (Octreoscan) preparation; Utilisation du systeme Take-set pour le radiomarquage des medicaments radiopharmaceutiques: exemple de la preparation du pentetreotide marque a l'indium 111 (Octreoscan)

    Energy Technology Data Exchange (ETDEWEB)

    Ben Reguiga, M.; Sinegre, M. [Hopital Beaujon, Unite de Radiopharmacie, Service de Pharmacie-toxicologie, 92 - Clichy (France); Besse, H.; Stievenart, J.L.; Le Guludec, D. [Hopital Beaujon, Service de Medecine Nucleaire92 - Clichy (France)


    The quality of indium 111 radiolabelled pentetreotide preparation (Octreoscan, Covidien) depends on several factors among which the use of a special transfer needle (Sterican) especially conceived to avoid the metal impurities introduction into the reactional medium during labelling. This device, usually provided by the supplier, can exceptionally present defects (twisted needle, folded bevel...) preventing its use for the preparation. In order to manage this risk, we propose in the present technical note an alternative labelling method, based on an adaptation of the original one and using another transfer device, the Take-setSWAN system, which permits to obtain high quality Octreoscan preparations that meet the product approval specifications. (authors)

  18. Facteurs liés au diagnostic tardif du cancer du sein: expérience du ...

    African Journals Online (AJOL)

    Le cancer du sein est le premier cancer féminin en termes d'incidence et de mortalité. Au Maroc, il vient au premier rang des cancers de la femme avant celui du col utérin Il constitue un problème de santé publique. Son pronostic est étroitement lié au stade auquel le diagnostic est posé. Il s'agit d'une pathologie dont les ...

  19. Science et technologie du collage

    CERN Document Server

    Cognard, Jacques


    L'Homme a su coller avant de savoir écrire et compter. Le collage est en effet l'une des premières techniques d'assemblage d'une structure que l'homme ait utilisée, technique qui n'est devenue science qu'au début du 20e siècle avec la fabrication des premières colles synthétiques. Cette science de l'adhésion a considérablement progressé ces dix dernières années et ce livre présente l'état actuel des connaissances en décrivant les progrès considérables réalisés dans la compréhension des phénomènes fondamentaux qui font qu'un collage tient bien et durablement. Après l'exposé des bases scientifiques nécessaires et des modèles théoriques récents, l'auteur introduit la distinction entre collages faibles et collages forts puis expose les difficultés rencontrées dans les cas pratiques, difficultés souvent dues à la complexité de la chimie des surfaces et des réactions surface-polymère. L'ouvrage se termine par la présentation de quelques applications récentes, en particulier dans...

  20. Coordonnateur du bureau d'assistance | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... d'utilisation des logiciels et du matériel informatique du Centre. Il tient un inventaire exact de l'équipement distribué à chaque membre du personnel et il travaille avec le personnel de la SSC à faire en sorte que les fichiers de maintenance contenus dans l'application de gestion du Bureau d'assistance soient tenus à jour.

  1. Au fond de la logique du lieu.

    Directory of Open Access Journals (Sweden)

    Augustin Berque


    Full Text Available L’école de Kyôto ( Kyôto gakuha est cette mouvance philosophique, née dans l’entre-deux-guerres, dont le Nihonshi jiten ( Dictionnaire historique du Japon des éditions Iwanami donne la présentation suivante : 1 École centrée sur la Faculté de philosophie de l’Université de Kyôto dans les années Taishô et Shôwa. Elle débuta autour de Nishida Kitarô et de Tanabe Hajime, mais on la connaît plutôt du fait que peu après le début de la guerre du Pacifique, en 1942, ...

  2. Le catch, la domestication du risque

    Directory of Open Access Journals (Sweden)

    Jeremy Lacoste


    Full Text Available Cet article vise à questionner les logiques de gestion, réappropriation et détournement du risque à travers le catch. Il s’appuie sur la monographie d’un club de la région parisienne observée durant huit mois. Parce que la discipline est à mi-chemin entre la sportivité théâtrale et la scénarisation pugilistique, elle pioche dans le registre de la représentation. Ainsi, art de la surenchère et du pastiche, le catch constitue un terreau majeur pour déconstruire les figures du risque.

  3. Cancer du Pancréas


    Billami, wahiba; Benbouziane, cherifa


    Le cancer du pancréas est un véritable problème de santé public. C’est un véritable drame pour le malade et son entourage. L’adénocarcinome canalaire reste la tumeur la plus fréquente du pancréas exocrine. Son traitement est basé sur la chirurgie. La chimiothérapie trouve sa place en situation adjuvante ainsi que pour les cancers à un stade localement avancé et métastatique. Notre étude incluant 57 cas étalée sur 5 ans au sein du service de chirurgie générale A CHU ...

  4. Progress report - fuel cell system of the L2ES 2000-2004; Rapport d'activites - systeme pile a combustible du L2ES 2000-2004

    Energy Technology Data Exchange (ETDEWEB)



    Fuel cell activities started in L2ES in 2000. Among all the definitions of the fuel cell system, the laboratory considers as the fuel cell generator, the stack, the ancillaries (air and hydrogen or more generally fuel supply, valves, humidifier or gas treatment, cooling), power electronics, buffer storage and control. The research topics at L2ES are: - fuel cell system modelling - fuel cell system architecture and its optimization - diagnosis and life time. All these topics are described into details. The perspectives and prospects of the laboratory at middle term are specified. (O.M.)

  5. Preliminary study on field buses for the control system of the high voltage of the ATLAS hadronic calorimeter; Etude preliminaire d`un reseau de terrain pour le systeme de controle des hautes tensions du calorimetre hadronique d`Atlas

    Energy Technology Data Exchange (ETDEWEB)

    Drevet, F.; Chadelas, R.; Montarou, G.


    We present here after a preliminary study on field buses for the control system of the high voltage of the photomultipliers of the TILECAL calorimeter. After some generalities, different commercial buses are reviewed (CAN, ARCET, WorldFIP, Profibus and LonWorks). The Profibus and LonWorks solution are more extensively studies as a possible solution for the high voltage system of the TILE hadronic calorimeter. (authors).

  6. Contribution to the study of hybrid ventilation system effectiveness in office and educational buildings; Contribution a l'etude de l'efficacite des systemes de ventilation hybride dans les batiments du secteur tertiaire

    Energy Technology Data Exchange (ETDEWEB)

    Cron, F.


    This study is part of the International Energy Agency Annex 35, 'Hybrid Ventilation in New and Retro-fitted Office Buildings', also called the HybVent Project. It presents the numerical analyses of a number of hybrid ventilation systems and their potential for several buildings in relation to local climate characteristics. The first part of this work reviews the state-of-the-art of existing ventilation systems and details the principles, expectations and features of hybrid ventilation systems. This first section also examines some examples of hybrid systems extracted from the collection of Annex 35 case studies. A comprehensive bibliography of numerical tools available for this type of analysis is given in the second section. The numerical models for building simulation and the object-oriented environment used to develop our simulation tool are presented in this section as well. Two single-zone studies undertaken in this project are reported in the third section. The first study concerns an experimental cell simulation. Numerical results are compared to experimental data to validate the use of the simulation tool developed. The second single-zone study predicts the potential of a specific hybrid ventilation system in a typical classroom, given climatic conditions. Finally, the last section presents a more complete study - a multi-zone study of the Tonga school, an Annex 35 case study located in Sweden. Three rooms were modelled initially to compare numerical results to experimental data and then to analyse the performance of the existing hybrid ventilation system and two other systems. This final study was completed for three European cities with very different climates. The overall analysis of the potential of the hybrid ventilation systems considered and the perspective for future work are outlined in the conclusion. (author)

  7. Message du président Nelson Mandela au CRDI à l'occasion du 25 ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    29 oct. 2010 ... Nous désirons exprimer toute notre gratitude au CRDI , ainsi qu'au gouvernement et à la population du Canada, pour l'aide qu'ils ont offerte, et qu'ils continuent d'offrir, à l'Afrique du Sud et aux autres pays en développement. Je me réjouis à l'idée de venir au Canada, et de peut-être visiter les bureaux du ...

  8. Profils des porteurs du VIH/SIDA au début du traitement ...

    African Journals Online (AJOL)

    But : Décrire les profils des porteurs de VIH/sida au début du traitement antirétroviral. Matériels et méthode: Les dossiers des porteurs du VIH/sida de la région maritime ont été analysés de mai 2008 à avril 2009 par le comité thérapeutique. Résultats: Parmi les 641 dossiers analysés, 67,40% venaient du district de Yoto.

  9. Transfert du Tendon du Muscle Jambier Postérieur : à propos de 24 ...

    African Journals Online (AJOL)

    De janvier 1981 à mars 2007, 24 patients souffrants de paralysie irréversible du SPE, ont bénéficié d'une chirurgie de transfert du tendon du muscle jambier postérieur. Les résultats fonctionnels de cette étude rétrospective ont été quantifiiés par la méthode d'évaluation fonctionnelle de STANMORE. Il s'agissait de 14 ...

  10. Quelle evolution de la situation du marche du travail en Algerie

    African Journals Online (AJOL)

    Les analyses « standards » de la participation au marché du travail ne prennent pas en compte la dynamique de celui-ci et donc, les effets de cycle de vie, de génération et de cycle économique se confondent, ce qui affecte la précision des résultats obtenus et masque d'autres réa-lités sur le fonctionnement du marché du ...

  11. Comparative analysis of operation and safety of subcritical nuclear systems and innovative critical reactors; Analyse comparative du fonctionnement et de la surete de systemes sous-critiques et de reacteurs critiques innovants

    Energy Technology Data Exchange (ETDEWEB)

    Bokov, P.M


    The main goal of this thesis work is to investigate the role of core subcriticality for safety enhancement of advanced nuclear systems, in particular, molten salt reactors, devoted to both energy production and waste incineration/transmutation. The inherent safety is considered as ultimate goal of this safety improvement. An attempt to apply a systematic approach for the analysis of the subcriticality contribution to inherent properties of hybrid system was performed. The results of this research prove that in many cases the subcriticality may improve radically the safety characteristics of nuclear reactors, and in some configurations it helps to reach the 'absolute' intrinsic safety. In any case, a proper choice of subcriticality level makes all analyzed transients considerably slower and monotonic. It was shown that the weakest point of the independent-source systems with respect to the intrinsic safety is thermohydraulic unprotected transients, while in the case of the coupled-source systems the excess reactivity/current insertion events remain a matter of concern. To overcome these inherent drawbacks a new principle of realization of a coupled sub-critical system (DENNY concept) is proposed. In addition, the ways to remedy some particular safety-related problems with the help of the core sub-criticality are demonstrated. A preliminary safety analysis of the fast-spectrum molten salt reactor (REBUS concept) is also carried out in this thesis work. Finally, the potential of the alternative (to spallation) neutron sources for application in hybrid systems is examined. (author)

  12. Du Bois in Transnational Perspective: The Loud Silencing of Black ...

    African Journals Online (AJOL)

    This article explores the 1920s thought of African-American intellectual and activist WEB Du Bois in regard to South Africa. It focuses on an influential 1924 article by WEB Du Bois, 'The Negro Mind Reaches Out', reprinted in Alain Locke's New Negro 1925 anthology. Despite Du Bois's Pan-Africanist ideology, and despite ...

  13. Gestion du Centre | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Lisa Woodward. Conseillère spéciale au Président. Ottawa, ON, Canada. Ce que nous faisons · Financement · Ressources · À propos du CRDI. Savoir. Innovation. Solutions. Carrières · Communiquez avec nous · Plan du site. Abonnez-vous à notre bulletin pour recevoir les nouvelles du CRDI chaque mois. Abonnez-vous.

  14. L’implication des organisations non gouvernementales dans les systèmes de santé des pays du Sud: l’exemple du Shisong Cardiac Centre The involvement of NGOs in the health systems in developing countries: the example of Shisong Cardiac Center

    Directory of Open Access Journals (Sweden)

    Butera Gianfranco


    Full Text Available Dans le tiers monde, près de 800 000 enfants naissent chaque année avec des cardiopathies congénitales (CC. Plusieurs millions d’enfants y vivent avec des CC corrigeables et une grande majorité décède à cause de l’absence de structure médicale spécialisée dans la correction chirurgicale totale ou palliative. C’est dans cette optique que l’association Associazione bambini cardiopatici nel mondo (Association mondiale des enfants cardiopathes a été créée. Grâce aux efforts de cette association, plus de 500 enfants souffrant de CC ont pu être évacués et soignés à l’hôpital de San Donato et plus 800 dans leur pays. Près de 200 praticiens en provenance des pays du Sud ont dans cette même perspective bénéficiée d’une formation en Italie. Cent quinze missions chirurgicales ont été réalisées dans différents pays: Egypte, Tunisie, Libye, Palestine, Cameroun, Roumanie, Azerbaïdjan, Pérou, Syrie, Yémen, Kurdistan et au Mali. Plusieurs hôpitaux ont été équipés gratuitement. Actuellement, l’association œuvre ardemment pour la réalisation de trois projets essentiels dont l’un est la construction d’un centre cardiaque dans la région de Shisong au Cameroun (Shisong Cardiac Centre. Ce centre cardiaque sera équipé d’appareils de dernière génération permettant de réaliser des investigations et la chirurgie cardiaques. L’inauguration de ce centre est prévue pour Novembre 2009.

  15. Les infirmiers du DUPA aux champs ?


    Matter catherine ... [et al.],


    Congrès de l'Union nationale pour la prévention du suicide (UNPS), Paris, février 2002 : Présentation de l'UNPS / Didier Camus - La privation de la relation et l'exclusion. La relation au travail / Catherine Matter - Violence et suicide / Dominique Papilloud - La relation aux soins: accueil des suicidants à l'hôpital / Fatima Belhadj - Congrès du GRAAP sur la psychose paranoïaque, 15-16 mais, Lausanne - 2ème congrès international "la réadaptation psychosociale intégrale dans et avec la commun...

  16. Le détachement du sens


    Rakotomalala, Jean Robert


    ABSTRACT The rule of the detachment of the meaning that we owe to Benoît de CORNULIER allows any semiotics to incorporate a sense already expressible in a given language. It allows among others to characterize the illocutionary acts. An expression is produced primarily to mean, but from the form of expression, we can agree on an attitude of the speaker. This form is both a Convention that highlights the rule of detachment of the meaning.; La règle du détachement du sens que nous devons à Beno...

  17. Webzzle : paradoxal confort du confinement.

    Directory of Open Access Journals (Sweden)

    Marc Dumont


    Full Text Available La plupart des agences de « news » technologiques viennent de se faire l’écho du développement de Webzzle , un nouveau moteur de recherche dit « collaboratif ». Ce cas est tout à fait intéressant parce qu’il permet de pointer rapidement quelques grands enjeux du Web 2 que risquent souvent de dissimuler la jungle sémantique et l’enthousiasme qui l’entourent. Pour quelles raisons ? Rappelons d’abord les deux principales perspectives ouvertes par cette nouvelle ...

  18. Une complication majeure du paludisme : le neuropaludisme


    Neveu, Natassia


    Le paludisme touche de nombreux pays dans le monde, particulièrement les régions tropicales et subtropicales défavorisées. C'est un fléau en Afrique sub-saharienne qui compte 90% du paludisme mondial. D'après les estimations actuelles, il y a eu 212millions cas d'infections palustres et 429000 décèspour l’année 2015. Le neuropaludisme est une complication grave et souvent mortelle du paludisme à Plasmodium falciparum. Il s'agit d'une urgence vitale surtout pour les populations les plus sensib...


    African Journals Online (AJOL)

    fertilisation est l'engrais chimique. Les engrais utilisés sont l'Urée (46 % N) et du KCl (60 % K). Des traitements nématicides et insecticides ont été effectués à partir du deuxième mois après plantation, puis tous les trois mois suivants grâce à un produit d'action mixte, le Furadan. 10G dont le principe actif est le carbofuran à.

  20. Du bonheur de la psychologie sociale


    Jouet, Josiane


    International audience; — Les contributions du dossier « Psychologie sociale, traitements et effets des médias » – dirigé par Claude Chabrol, Didier Courbet, Marie-Pierre Fourquet-Courbet et publié par Questions de communication (6, 2004) – sont examinées dans une perspective critique qui discute les qualités et les limites des travaux expérimentaux sur les effets des médias. L'intérêt des apports transdisciplinaires ouvre de nouvelles pistes à l'étude du conditionnement des individus sans po...

  1. Essai de synthèse stratigraphique et palynologique du système dévonien en Lybie occidentale Stratigraphic and Palynological Synthesis Attempt of the Denovian System in Western Libya

    Directory of Open Access Journals (Sweden)

    Massa D.


    Full Text Available De nombreux sondages profonds ont été réalisés lors des recherches pétrolières en Libye occidentale (bassin de Rhadamès. Simultanément, les zones d'affleurement ont été étudiées avec soin. Dans le présent travail, les auteurs présentent des conclusions stratigraphiques et palynologiques nouvelles. Les cycles sédimentaires qui constituent le Dévonien ont une épaisseur qui n'excède pas le millier de mètres. Ils sont subdivisés en sept formations qui couvrent l'intervalle stratigraphique allant du Praguien au Strunien. Ainsi, le Couvinien, le Givétien, le Frasnien et le Famennien ont été différenciés. Le Dévonien basal (Lochkovien est absent. Les corrélations latérales pour l'ensemble du bassin considéré sont satisfaisantes. Une trentaine de sondages ont été étudiés en palynologie. Ils ont montré une extraordinaire richesse en spores dans tout le Dévonien. II a été possible de différencier onze Palynozones, à partir des Spores et Chitinozoaires présents. En comparant les faunes et les microflores dévoniennes présentes en Libye, à celles connues en Europe, en Afrique du Nord et en d'autres régions, on obtient une biostratigraphie homogène et cohérente. Numerous deep boreholes have been drilled during oil prospection in western Libya (Rhadames Basin. At the same time, outcrop areas have been carefully examined. This article gives new stratigraphic and palynological conclusions. The sedimentary cycles making up the Devonian are no more than 1,000 meters thick. They are subdivided into seven formations, covering the stratigraphic interval from the Praguian ta the Strunian. In this way, the Couvinian, Givetian, Frasnian and Famennian are differentiated. The lowermost part of the Devonian (Lochkovian is missing. The lateral corrélations for the entire basin being considered are satisfactory. The palynology has been numerous in the Devonian. Eleven polynozones are differentiated on the basis of spores and

  2. L’ombre du Condor

    Directory of Open Access Journals (Sweden)

    Franck Gaudichaud


    Full Text Available Si l’on observe aujourd’hui, avec un regard d’historien, le Cône Sud à la fin des années soixante soixante-dix et que par, la suite, on tourne une page de quelques années, pour fixer la même partie du monde, le constat est sans appel : le sous-continent latino-américain est passé, en général, d’une phase de forte mobilisation et politisation sociale, montée en puissance de partis et organisations révolutionnaires, surgissements de gouvernements populistes de gauche ou progressistes – appelant à une rupture avec l’impérialisme –, à un reflux généralisé du mouvement ouvrier, une ère de violence politique étatisée, la destruction massive de tous les espaces d’expression et de participation démocratiques, l’écrasement physique et idéologique sans relâche des militants et mouvements révolutionnaires, la mise en place de modèles économiques capitalistes dirigistes puis/ou néolibéraux. Que s’est-il passé ? Quel fil conducteur a été rompu et par quels moyens l’Amérique Latine a été conduite à ce reflux des luttes sociales ? Pour répondre à cette question, nous nous contenterons dans cet article de focaliser notre objectif sur un aspect spécifique et essentiel de ce retournement de tendance que vit alors l���Amérique Latine : celui de la mise en place d’un terrorisme d’Etat contre-révolutionnaire transnational, appuyé par l’impérialisme américain, nommé « l’Opération Condor ». Dans cet article, il s’agit de montrer le fonctionnement d’un terrorisme hégémonique dont l’impact sur les sociétés latino-américaines est considérable en termes de destruction du mouvement social, des activités culturelles et de l’ensemble de la vie sociale et politique qui restent marquées, encore aujourd’hui à des degrés divers, par la violence, la peur et l’atomisation structurelle.Si miramos hoy en día, desde una perspectiva histórica, el Cono Sur hacia finales de

  3. Evaporation of the {sup 36} Ar + {sup 58} Ni system studied with the 4{pi} INDRA detector; Vaporisation du systeme {sup 36} Ar + {sup 58} Ni etudie avec le detecteur 4{pi} INDRA

    Energy Technology Data Exchange (ETDEWEB)

    Ouatizerga, A.


    With the capabilities of the INDRA 4{pi} detector, vaporization of the {sup 36}Ar + {sup 58}Ni system has been detected permitting thus first studies. This phenomenon which corresponds to a total disintegration of the total nuclear system into neutrons and isotopes of H is expected for very high excitation energies induced in-nucleus collisions at intermediate energies. The onset of vaporization was determined, it is found at 52 MeV per nucleon, which corresponds to an available energy per nucleon into the system of 12.3 MeV. Temperature at which vaporization occurs is a crucial parameter to describe the phenomenon. Upper limits for temperature have been deduced within two frameworks (complete surface evaporation or calculation within the free gas limit) and lead to values in the range 7-10 MeV. (author). 43 refs.

  4. Le Flaubert de Charles Du Bos

    Directory of Open Access Journals (Sweden)

    Jacques Neefs


    Full Text Available Charles Du Bos a porté une attention constante à l’œuvre de Flaubert (à l’exclusion de Bouvard et Pécuchet qui semble ne pas exister pour lui, à Madame Bovary et à L’Éducation sentimentale en particulier. La mise en relation de son étude : « Sur le milieu intérieur chez Flaubert », écrite en 1921, avec des textes du Journal de 1923 et de 1937, les rapprochements avec Gogol, Thomas Hardy, Tolstoï, Baudelaire, Henry James qui traversent les écrits de Du Bos, permettent de suivre ce que celui-ci décrit comme « l’expérience spirituelle » d’une matérialité comprise dans la conquête de la triple exigence du Beau, du Vivant et du Vrai. Du Bos décèle la force de l’œuvre de Flaubert dans la « disproportion » du style, et dans la puissance d’absorption qui fait la densité de cette prose, et qui désigne un extraordinaire travail de conversion. L’obscure expérience spirituelle ainsi poursuivie est celle d’un absolu de l’art, expérience paradoxale d’un « mystique qui ne croit à rien » (comme se désignait Flaubert lui-même, que le critique lie à une interrogation sur sa propre conversion.Charles Du Bos devoted an unflagging attention to Flaubert’s work (except for Bouvard et Pécuchet, which, apparently, according to him did not exist, to Madame Bovary and in particular L’Éducation sentimentale. The connection between his essay “Sur le milieu intérieur chez Flaubert”, written in 1921, and extracts from his Journal, from 1923 to 1937, the comparisons with Gogol, Thomas Hardy, Tolstoy, Baudelaire, and Henry James that run through the writings of Du Bos, allow us to follow what he terms “the spiritual experience” of a materiality encompassed in the conquest of the triple demand of the Beautiful, the Living, the Truth. Du Bos detects the power of Flaubert’s work in the “disproportion” of his style, and the power of absorption that forms the density of his prose, showing an

  5. B1 but not B2 bradykinin receptor agonists promote DU145 prostate ...

    African Journals Online (AJOL)

    Background: The kallikrein-kinin system (KKS) is an endogenous pathway involved in angiogenesis and tumourigenesis, both vital for cancer growth and progression. Objectives: To investigate the effect of two bradykinin receptor (B1R and B2R) agonists on growth and motility of prostate tumour (DU145) and ...

  6. Sud du Sahara | Page 54 | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Read more about Examen de la mortalité maternelle pour le renforcement du système de santé au Kenya et au Zimbabwe. Langue French. Read more about Strengthening the Health System through a Maternal Death Review (Kenya and Zimbabwe). Langue English. Read more about Taxes sur les cigarettes en Tanzanie.

  7. Office Automation Pilot: A Paperless Approach at College of DuPage. (United States)

    Carlson, Bart

    The pilot project described in this report was undertaken by the College of DuPage (CD) to increase the clerical efficiency of seven administrative offices through the installation of a computerized word processing and data transmission system. The first section of the report provides background information detailing: the history of computer…

  8. ALIBABA, an assistance system for the detection of confinement leaks in a PWR reactor; ALIBABA, un systeme d`aide a la detection des voies de fuites du confinement sur un reacteur a eau sous pression

    Energy Technology Data Exchange (ETDEWEB)

    Bedier, P.O.; Libmann, M. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Mecanique et de Technologie


    The objective of the Crisis Technical Center (CTC) of the French Institute for Nuclear Protection and Safety (IPSN) is to estimates the consequences of a given nuclear accident on the populations and the environment. ALIBABA is a data processing tool available at the CTC and devoted to the detection of confinement leaks in 900 MWe PWR reactors using the activity values measured by the captors of the installation. The heart of this expert system is a structural and functional representation of the different components directly involved in the leak detection (isolating valves, ventilation systems, electric boards etc..). This tool can manage the availability of each component to make qualitative and quantitative balance-sheets. This paper presents the ALIBABA software, an industrial prototype realized with the SPIRAL knowledge base systems generator at the CEA Reactor Studies and Applied Mathematics Service (SERMA) and commercialized by CRIL-Ingenierie Society. It describes the techniques used for the modeling of PWR systems and for the visualization of the survey. The functionality of the man-machine interface is discussed and the means used for the validation of the software are summarized. (J.S.). 6 refs.

  9. Information report on greenhouse gas emission trading systems, in the name of the Sustainable Development and Land Management Commission; Rapport d'information depose en application de l'article 145 du reglement par la mission d'information sur les marches de quotas de gaz a effet de serre au nom de la commission du developpement durable et de l'amenagement du territoire

    Energy Technology Data Exchange (ETDEWEB)



    In its first part, this report explains the choice of trading systems due to the impossibility to define taxes on carbon emissions and to the influence of international negotiations. It also describes the operation of the European system with its three allocation phases (an experimental framework between 2005 and 2007, an actually constraining framework between 2008 and 2012, and a sustainable frame from 2013), and outlines the realistic character of emission reduction objective by 2020. It identifies and discusses the weaknesses of the European system, notably due to an insufficiently regulated market and to a partial taking into account of emission. The second part identifies ways to improve the system by extending it (including new sectors, taking some particular emissions into account, and valuing carbon sequestration), by preserving the competitiveness of European industries, and by aiming at the construction of a de-carbonated Europe

  10. Watershed Data Management (WDM) database for West Branch DuPage River streamflow simulation, DuPage County, Illinois, January 1, 2007, through September 30, 2013 (United States)

    Bera, Maitreyee


    The U.S. Geological Survey (USGS), in cooperation with the DuPage County Stormwater Management Department, maintains a database of hourly meteorological and hydrologic data for use in a near real-time streamflow simulation system. This system is used in the management and operation of reservoirs and other flood-control structures in the West Branch DuPage River watershed in DuPage County, Illinois. The majority of the precipitation data are collected from a tipping-bucket rain-gage network located in and near DuPage County. The other meteorological data (air temperature, dewpoint temperature, wind speed, and solar radiation) are collected at Argonne National Laboratory in Argonne, Ill. Potential evapotranspiration is computed from the meteorological data using the computer program LXPET (Lamoreux Potential Evapotranspiration). The hydrologic data (water-surface elevation [stage] and discharge) are collected at U.S.Geological Survey streamflow-gaging stations in and around DuPage County. These data are stored in a Watershed Data Management (WDM) database.This report describes a version of the WDM database that is quality-assured and quality-controlled annually to ensure datasets are complete and accurate. This database is named WBDR13.WDM. It contains data from January 1, 2007, through September 30, 2013. Each precipitation dataset may have time periods of inaccurate data. This report describes the methods used to estimate the data for the periods of missing, erroneous, or snowfall-affected data and thereby improve the accuracy of these data. The other meteorological datasets are described in detail in Over and others (2010), and the hydrologic datasets in the database are fully described in the online USGS annual water data reports for Illinois (U.S. Geological Survey, 2016) and, therefore, are described in less detail than the precipitation datasets in this report.

  11. 35 Typologie des eaux de surface du bassin du Sebou par multi ...

    African Journals Online (AJOL)


    Résumé. L'étude consiste en l'utilisation de la démarche biologique, particulièrement les indices IBGN et IBG-RCS, pour montrer l'évolution de la qualité biologique des eaux de surface du bassin du Sebou dans la période. 2009-2011. La caractérisation physicochimique/microbiologique de la qualité des eaux de surface ...

  12. Établissement du programme de recherche 2012-2014 du réseau d ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Faisant fond sur le travail accompli au cours de cette première phase, les chercheurs évalueront les priorités parmi les problèmes les plus urgents et élaboreront une feuille de route ... La demande de mesures novatrices, mais rigoureuses, du bien-être national allant au-delà du produit national brut ne cesse de croître.

  13. Réglementation du marché du travail en Chine : politiques en ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Réglementation du marché du travail en Chine : politiques en matière de salaire minimum. Ce projet de recherche portant sur les politiques en matière de salaire minimum en Chine est entrepris afin d'évaluer les effets de ces politiques et de fournir des lignes directrices aux organes de réglementation à l'échelon ...

  14. Lignes directrices du CRDI pour la préparation du rapport technique ...

    International Development Research Centre (IDRC) Digital Library (Canada)


    Au terme d'un projet, les bénéficiaires d'une subvention du CRDI doivent remettre un rapport technique final pour que le CRDI puisse débloquer le dernier versement de la subvention. Ce rapport fournira le détail des activités financées dans le cadre du projet et en relèvera les réalisations importantes et les leçons ...

  15. Perception locale de l'évolution du milieu à Oula au Nord du Burkina ...

    African Journals Online (AJOL)

    d'adaptation. La présente étude a été réalisée dans la région du Nord du Burkina Faso. La méthodologie s'est basée sur des observations de terrain, et des enquêtes ... Mots clés : Perception des populations locales, changements environnementaux, stratégie d'adaptation, Oula, ..... pierreux, sur la réhabilitation de la.

  16. Un gagnant du concours de photos du CRDI partage son prix avec ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    28 janv. 2011 ... À l'été 2006, le CRDI a invité les photographes amateurs et professionnels du monde entier à montrer les moyens créatifs qu'utilisent les habitants des villes des pays du Sud ou en développement pour relever les défis de la vie urbaine. ... Sexual abuse and access to justice for rural women in West Africa.

  17. Description du peuplement piscicole du lac sahélien de Higa, un ...

    African Journals Online (AJOL)


    30 nov. 2015 ... niloticus et Heterotis niloticus soient confondus à d'autres espèces permettent une certaine crédibilité sur la disparition de ces deux espèces. Cette extinction locale n'est pas étonnante car Higa est dégradé au point que les pêcheurs professionnels venus du Mali et du Niger depuis. 4 décennies ont pour la ...

  18. Consolidation du leadership en recherche en écosanté en Asie du ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    25 avr. 2016 ... Financée par le CRDI, l'Initiative de renforcement du leadership en recherche en écosanté en Asie du Sud‑Est appuie l'exécution, dans la région, de travaux de recherche qui visent à permettre de mieux comprendre les effets de la transformation de l'agriculture sur les écosystèmes et sur la santé humaine ...

  19. 69 Optimisation du procédé de fabrication du tchapalo, bière ...

    African Journals Online (AJOL)


    physico-chimique d'une bière traditionnelle ouest Africaine : le tchapalo". Agronomie Africaine XVII. (2) (2005) 73-162. [7] - J. PERISSE, J. ADRIAN, A. RERAT et S. LE BERRE, "Bilan nutritif de la transformation du sorgho en bière ; préparation, composition, consommation d'une bière du Togo", Annales de la nutrition et de.

  20. Une force venant du futur chercherait à saboter l'expérience du LHC...

    CERN Multimedia

    Scappaticci, Bruno


    "A quelques jours de la reprise de l'expérience du LHC, on ne parle plus de la menace d'un trou noir, mais deux physicines souteinnent aujourd'hui une tout autres hypothèse: la nature, depuis le futur, va continuer de contrarier les travaux du Cern, afin d'éviter à l'Homme de mettre le doigt là où il ne faut pas..." (1.5 pages)

  1. Accroissement de la compétitivité du Kenya dans l'économie du ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    L'University Research Chairs Programme sera le premier du genre au Kenya. Il est explicitement conçu pour aider les universités publiques locales à forger des alliances stratégiques avec l'industrie dans les secteurs de l'agriculture et de la santé. Les fonds du CRDI permettront à la NACOSTI de financer une chaire durant ...

  2. Effets du travail du sol sur le comportement chimique et biologique ...

    African Journals Online (AJOL)


    31 juil. 2017 ... RESUME. Objectif : L'objectif de cette étude est de comparer les effets de six techniques culturales de mise en place du blé tendre sur certaines propriétés chimiques et biologiques du sol et les conséquences sur le rendement grain et ses composantes dans la région «non chernozem» en 7ème années ...

  3. Les tons du langage tambouriné et les tons du langage parlé. Texte ...

    African Journals Online (AJOL)

    A.J. Smet présente, en traduction française, un texte inédit de Placide Tempels concernant la relation entre les tons du langage tambouriné et ceux du langage parlé. Ce texte est à l'origine de la correspondance entre Tempels et Hulstaert. Cette correspondance a eu une influence certaine sur la rédaction de la ...

  4. Metallurgical study and phase diagram calculations of the Zr-Nb-Fe-(Sn,O) system; Etude metallurgique et calculs des diagrammes de phases des alliages base zirconium du systeme: Zr-Nb-Fe-(O,Sn)

    Energy Technology Data Exchange (ETDEWEB)

    Toffolon, C. [CEA/Saclay, Dept. d' Etudes du Comportement des Materiaux (DECM), 91 - Gif-sur-Yvette (France)]|[Paris-6 Univ., 75 (France)


    The Framatome M5{sup TM} Zr-Nb-O alloy with small amounts of Fe is of interest for nuclear applications (PWR fuel cladding).The behaviour of this kind of alloy for in-service conditions strongly depends on the microstructure. Therefore, a metallurgical study of alloys of the Zr-Nb-Fe-(O-Sn) system has been developed in order to study the influence of chemical composition variabilities of Nb, Fe and O and thermal treatments on the resultant microstructure. In order to get some insight on the physical metallurgy of Zr-Nb-Fe-(Sn,O) alloys and to minimize the experiments, it is useful to build a thermodynamic database. With this object, it was necessary to re-optimize and to calculate the low order binary systems such as Fe-Nb and Nb-Sn in order to assess the Zr-Nb-Fe-(Sn,O) system. Then, the experimental studies concerned: the influence of small variations in Nb and O contents on the {alpha}/{beta} transus temperatures. A comparison between experimental results and thermodynamic predictions showed a good agreement; the precipitation kinetics of {beta}Nb and intermetallic phases in the {alpha} phase domain. These experiments showed that the kinetics depends on the initial metallurgical conditions; the determination of the crystallographic structure and the stoichiometry of the ternary Zr-Nb-Fe intermetallic compounds as a function of the temperature. Finally, these experimental data were used to propose a first assessment of the Zr-Nb-Fe(O{approx}1200 ppm) system. (author)

  5. les cahiers du cread - Vol 95 (2011)

    African Journals Online (AJOL)

    Impact du secteur financier sur l'activite economique en Algerie par une MCSRF · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... Contrat et confiance pour la gestion des risques de defaillances dans les transactions de financement informel dans le secteur agricole en Algerie · EMAIL FREE FULL TEXT EMAIL FREE ...

  6. Mort du taylorisme et recul des syndicats


    Ruffier, Jean


    International audience; L'article décrit comment le taylorisme a cru et a disparu en France. Il met en relation la disparition du taylorisme avec une difficulté de plus en plus grande pour les syndicats de s'implanter dans les entreprises

  7. Learning about Cri du Chat Syndrome (United States)

    ... arm. The clinical symptoms of cri du chat syndrome usually include a high-pitched cat-like cry, mental retardation, delayed development, distinctive facial features, small head size (microcephaly), widely-spaced eyes (hypertelorism), low birth weight and weak muscle tone ( ...

  8. La Carte du Ciel vue de Potsdam (United States)

    Bigg, C.


    Ce chapitre s'attache à retracer les aléas de la Carte du Ciel depuis l'Observatoire de Potsdam. Le cas de cet établissement sert de point de comparaison avec les observatoires français, en particulier en ce qui concerne l'organisation du travail au sein de l'Observatoire et le problème de la répartition des ressources humaines et instrumentales dans la poursuite de ce projet de longue haleine. On remarque notamment que si les astronomes allemands, héritiers d'une tradition d'excellence en astrométrie stellaire, se devaient de prendre part à la "Photographische Himmelskarte", ce ne sont pas les observatoires d'astronomie classique mais le nouvel observatoire d'astrophysique de Potsdam qui entreprendra cette tâche. Les études effectuées dans ce cadre sur la photographie stellaire et surtout la photométrie photographique seront ainsi considérées comme des contributions à la jeune science astrophysique - du moins jusqu'à ce que la Carte du Ciel entre en concurrence dans l'Observatoire avec les projets de catalogues de spectres stellaires et de mesure des vitesses radiales.

  9. Insectes ravageurs du piment Capsicum chinense Jacq ...

    African Journals Online (AJOL)


    30 sept. 2015 ... (Solanaceae) à Port-Bouët (Abidjan-Côte d'Ivoire) : Pratiques de lutte par les pesticides chimiques. 8667. Insectes ravageurs du piment Capsicum chinense. Jacq. (Solanaceae) à Port-Bouët (Abidjan-Côte d'Ivoire) : Pratiques de lutte par les pesticides chimiques. AKESSE E. N.1*, OUALI-N'GORAN S-W.

  10. Maison du Peuple; Jean Prouvè

    DEFF Research Database (Denmark)

    Beim, Anne


    The chapter presents a deep analysis of Maison du Peuple, de Clichy in Paris by the French architect and manufacturer Jean Prouvè. The historical context of the original ideas behind the building and how and by which means it was constructed are being examined from a tectonic perspective....

  11. Growth study of cri du chat syndrome (United States)

    Collins, M; Eaton-Evans, J


    We compared the growth of children with cri du chat (5p−) syndrome with the 1990 UK growth curves. Most subjects had impaired growth, particularly of head circumference. The more emaciated the child the more pronounced the microcephaly, showing the need for growth and nutrition monitoring.


  12. Sign Communication in Cri du Chat Syndrome (United States)

    Erlenkamp, Sonja; Kristoffersen, Kristian Emil


    This paper presents findings from a study on the use of sign supported Norwegian (SSN) in two individuals with Cri du chat syndrome (CCS). The study gives a first account of some selected aspects of production and intelligibility of SSN in CCS. Possible deviance in manual parameters, in particular inter- and/or intra-subject variation in the use…

  13. Bourses : Formulaire - Rapport final du boursier

    International Development Research Centre (IDRC) Digital Library (Canada)

    Foresterie communautaire : l'arbre au service de l'humanité - bourse John G. Bene. ☐ Bourse de recherche Bentley sur les systèmes culturaux. ☐ Bourse du CRDI aux chercheurs candidats au doctorat. Nom : Sujet de recherche : Établissement(s) de rattachement : Directeur de thèse ou de mémoire : Programme d'études :.

  14. activite photosynthetique du phytoplancton dans le barrage ...

    African Journals Online (AJOL)


    Ce plancton végétal y assure essentiellement la production primaire. Cette ressource trophique est exploitée notamment par la microfaune planctonique et par certains poissons du lac. Ces derniers constituent dans cet écosystème lacustre une ressource économique importante (Gourène et al., 1999). Les travaux portant ...


    Directory of Open Access Journals (Sweden)

    Malika CHIKHI


    Full Text Available Cet article présente  le modèle linéaire généralisé englobant les  techniques de modélisation telles que la régression linéaire, la régression logistique, la régression  log linéaire et la régression  de Poisson . On Commence par la présentation des modèles  des lois exponentielles pour ensuite estimer les paramètres du modèle par la méthode du maximum de vraisemblance. Par la suite on teste les coefficients du modèle pour voir leurs significations et leurs intervalles de confiances, en utilisant le test de Wald qui porte sur la signification  de la vraie valeur du paramètre  basé sur l'estimation de l'échantillon.

  16. Demande de subvention de recherche du CRDI

    International Development Research Centre (IDRC) Digital Library (Canada)

    Si les documents originaux ne sont pas disponibles dans l'une de ces langues, une traduction certifiée conforme doit être fournie avec une copie du document original. Une traduction certifiée conforme signifie que le document est établi par un traducteur externe (qui ne fait pas partie de votre organisme) attestant que la ...

  17. Interface morphosyntaxique du nom en agni

    Directory of Open Access Journals (Sweden)

    Amoikon Dyhie Assanvo


    Full Text Available Les études portant sur le nom touchent divers domaines dont les sciences du langage. Dans la perspective de la grammaire générative et transformationnelle, les puristes soutiennent que dans un énoncé verbal, le nom est l’un des constituants le plus dynamique à identifier ; et pour cause, il a deux traits sémantiques : -animé et +animé. Bien plus, dans un syntagme nominal, la détermination du nom peut se faire soit par des spécificateurs fonctionnels, soit par un génitif adjectival ou déterminatif. Dans la présente étude, en s’appuyant sur le courant de la morphologie générative, l’on constate que la formation du nom peut être le résultat de procédés flexionnels, dérivationnels et compositionnels. Si la flexion et la dérivation concernent plus la morphologie, en revanche, le procédé de la composition se situe à l’interface morphologie et syntaxe. À ce niveau, le défi à relever consiste à délimiter la frontière entre syntagmes complexe, génitif et figé, puisque les trois constructions utilisent le même procédé pour la formation du syntagme nominal.

  18. Les écueils du double jeu

    Directory of Open Access Journals (Sweden)

    Séverin Muller


    Full Text Available À partir d’une expérience d’observation participante qui a consisté à travailler comme ouvrier puis à intégrer les bureaux de la direction dans un abattoir industriel, l’auteur étudie les effets cognitifs de la transgression d’un ordre établi dans l’entreprise : les ouvriers avec les ouvriers et les cadres avec les cadres. Les situations décrites de rupture avec les usages du milieu permettent de réfléchir aux façons d’être identifié selon les circonstances, aux formes d’instrumentalisation du chercheur sur son terrain, aux manières dont la confiance s’acquiert se maintient ou se perd dans un environnement conflictuel (selon quels critères propres à chaque groupe sommes nous acceptés, cooptés ou exclus ?. Finalement, la démarche transversale et le jeu transgressif qu’elle suggère participent à l’apprentissage des systèmes de référence non partagés au sein d’une même organisation.Starting from an experiment of participating observation which consisted in working as worker then to integrate the offices of the direction in an industrial slaughterhouse, the author studies the cognitive effects of the transgression of an order established in the company: workers with the workers and managers with the managers. The described situations of rupture with the uses of the social sphere make it possible to think of the ways of being identified according to circumstances, the shapes of « instrumentalisation » of the researcher on his field, with the ways in which confidence is acquired, is maintained or lost in a conflict environment (according to which specific criteria to each group are us accepted, co-opted or excluded?. Finally, the transverse way and the transgressive play that it suggests take part in the training of the systems of reference not shared in the same organization.A partir de un experimento de observación participante que consistió en trabajar como obrero antes de integrar las

  19. Study of the tributyl phosphate - 30% dodecane solvent; Etude du solvant phosphate tributylique - 30 % dodecane

    Energy Technology Data Exchange (ETDEWEB)

    Leroy, P. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses. Centre d' Etudes Nucleaires, 92 (France)


    This study, originating mainly from a literature survey, gives the principal chemical and physical features of the tributyl-phosphate (TBP) agent diluted at 30 volumes per cent in dodecane. The mixture is a very commonly used extractant in nuclear fuel processing. In this paper, the main following points are reported: -) the components (TBP and diluents) -) the TBP-diluents systems (non-loaded), -) the TBP-diluents-water systems, -) TBP-diluents-water-nitric acid systems, and -) industrial solvents. (author) [French] Cette etude, d'origine bibliographique, regroupe les caracteristiques physico-chimiques essentielles du phosphate tributylique (TBP) dilue a 30% en volume dans du dodecane. Ce melange constitue un agent d'extraction tres utilise dans le traitement des combustibles nucleaires. Les principaux points traites sont les suivants: -) les constituants (TBP et diluants), -) les systemes TBP-diluants non charges, -) les systemes TBP-diluants-eau, -) les systemes TBP-diluants-eau-acide nitrique, et -) les solvants industriels. (auteur)

  20. Néotoponymie contestée à Potchefstroom / Tlokwe (Province du Nord-Ouest, Afrique du Sud

    Directory of Open Access Journals (Sweden)

    Myriam Houssay-Holzschuch


    Full Text Available Illustration 1 - Extrait du site de la municipalité de Potchefstroom / TlokweSource : 2- Potchefstroom (Afrique du SudAuteur : Béatrice Obry-Guyot, début décembre 2007.Illustration 3 - Extrait du plan Google Map de PotchefstroomSource : Google Map.La question toponymique en Afrique du Sud sur la longue durée porte sur deux thèmes essentiels porteurs de controverses : le plurilinguisme et le marquage symbolique et mémoriel du te...

  1. Soil gas measurements around the most recent volcanic system of metropolitan France (Lake Pavin, Massif Central); Mesure des gaz des sols autour du systeme volcanique le plus recent de France metropolitaine (lac Pavin, Massif Central)

    Energy Technology Data Exchange (ETDEWEB)

    Gal, F. [BRGM, Metrology, Monitoring and Analysis Division, 3, avenue Claude-Guillemin, 45060 Orleans cedex 02 (France); Gadalia, A. [BRGM, Department of Geothermal Energy, 3, avenue Claude-Guillemin, 45060 Orleans cedex 02 (France)


    Soil gas monitoring techniques (CO{sub 2}, O{sub 2}, {sup 222}Rn, {sup 4}He) are used in the geographical context of the recent volcanic system of Lake Pavin (Puy-de-Dome), to get a better knowledge of local gaseous emissions, in order to establish whether or not this system can present evidence of reactivation. Concentrations up to 100% CO{sub 2} and 50 ppm of helium are measured in a narrow geographical area (Escarot Mofette), together with a magmatic origin for these gases. Radon activity in the Mofette area is quite high, but does not show, compared to surrounding areas, enrichments as high as those measured for CO{sub 2} or helium. Hourly records of these radon activities, performed during several weeks, suggest the existence of pulsed radon exhalation in the Mofette area. The period of this pulsation is around 40 days but its origin remains poorly understood. Apart from this Mofette, no evidence of gas originating from depth is highlighted. (authors)

  2. Transportation network development in a liberalized power system, a coordination problem with production; Le developpement du reseau de transport dans un systeme electrique liberalise, un probleme de coordination avec la production

    Energy Technology Data Exchange (ETDEWEB)

    Rious, V


    This thesis analyses how the long term coordination between generation and transmission is organised in a liberalized power system. It relies on a modular analysis framework that allows us to separate the coordination mechanisms into distinct modules. The governance structure of the power network completes this analysis framework. In a rationale of institutional complementarity, this governance structure impacts the way the TSOs effectively implement the power flow management. The proactive behaviour of the TSO for the coordination of generation and transmission can be explained in two ways. Firstly, the network investment can be the only effective process of long term coordination between generation and transmission. Secondly, when forecasting network investment, the TSO can prepare the accommodation of the fast-built generation units and detect the potential failures of locational incentive signals. (author)

  3. Quality control of the treatment planning systems dose calculations in external radiation therapy using the Penelope Monte Carlo code; Controle qualite des systemes de planification dosimetrique des traitements en radiotherapie externe au moyen du code Monte-Carlo Penelope

    Energy Technology Data Exchange (ETDEWEB)

    Blazy-Aubignac, L


    The treatment planning systems (T.P.S.) occupy a key position in the radiotherapy service: they realize the projected calculation of the dose distribution and the treatment duration. Traditionally, the quality control of the calculated distribution doses relies on their comparisons with dose distributions measured under the device of treatment. This thesis proposes to substitute these dosimetry measures to the profile of reference dosimetry calculations got by the Penelope Monte-Carlo code. The Monte-Carlo simulations give a broad choice of test configurations and allow to envisage a quality control of dosimetry aspects of T.P.S. without monopolizing the treatment devices. This quality control, based on the Monte-Carlo simulations has been tested on a clinical T.P.S. and has allowed to simplify the quality procedures of the T.P.S.. This quality control, in depth, more precise and simpler to implement could be generalized to every center of radiotherapy. (N.C.)

  4. Hygro-thermo-aeraulic modeling of multi-zone buildings. Proposal of a strategy for the resolution of the coupled system; Modelisation hygro-thermo-aeraulique des batiments multizones. Proposition d'une strategie de resolution du systeme couple

    Energy Technology Data Exchange (ETDEWEB)

    Woloszyn-Vallon, M.


    hygroscopic materials. Experimental conditions are simulated using the proposed model. A good overall performance of the model can be sent, if the vapour absorbed and released by building materials is represented. We are interested in a dynamic system described by a set of algebro-diffeential equations. To start the time integration of the differential equations, we need to fix a starting point. The best choice is given by the solution of the set of non-linear algebraic equations corresponding to initial stationary date. However, the resolution of this set is often found impossible. To face the numerical resolution of our problems, we called on block methods. The idea is to divide judiciously the system of equations, in such a way as to get an easy solution for each block. The physical interactions between blocks are then numerically ensured by successive global interations. After analysing our system, three principal blocks are identified: airflow block, describing inter-zonal air movements; energy block, describing temperatures in each zone; moisture block, describing air moisture content in each zone. The airflow block has a special role; it determines moisture and energy transport. A strategy to update the values of variables is also adapted to physical interaction among systems and allows rapid convergence. The proposed strategy is validated on a set of benchmark tests designed by CLIM2000 users. (author)

  5. Mathematical and numerical analysis of the resistive magnetohydrodynamics system with self-generated magnetic field terms; Analyse mathematique et numerique du systeme de la magnetohydrodynamique resistive avec termes de champ magnetique auto-genere

    Energy Technology Data Exchange (ETDEWEB)

    Wolff, Marc


    This work is devoted to the construction of numerical methods that allow the accurate simulation of inertial confinement fusion (ICF) implosion processes by taking self-generated magnetic field terms into account. In the sequel, we first derive a two-temperature resistive magnetohydrodynamics model and describe the considered closure relations. The resulting system of equations is then split in several subsystems according to the nature of the underlying mathematical operator. Adequate numerical methods are then proposed for each of these subsystems. Particular attention is paid to the development of finite volume schemes for the hyperbolic operator which actually is the hydrodynamics or ideal magnetohydrodynamics system depending on whether magnetic fields are considered or not. More precisely, a new class of high-order accurate dimensionally split schemes for structured meshes is proposed using the Lagrange re-map formalism. One of these schemes' most innovative features is that they have been designed in order to take advantage of modern massively parallel computer architectures. This property can for example be illustrated by the dimensionally split approach or the use of artificial viscosity techniques and is practically highlighted by sequential performance and parallel efficiency figures. Hyperbolic schemes are then combined with finite volume methods for dealing with the thermal and resistive conduction operators and taking magnetic field generation into account. In order to study the characteristics and effects of self-generated magnetic field terms, simulation results are finally proposed with the complete two-temperature resistive magnetohydrodynamics model on a test problem that represents the state of an ICF capsule at the beginning of the deceleration phase. (author)

  6. Impact factors on the structuration and the rheological behavior of the clay-water system for smectite dispersions; Facteurs determinant l'organisation et la rheologie du systeme argile-eau pour des suspensions de smectites

    Energy Technology Data Exchange (ETDEWEB)

    Paumier, S


    Smectite are swelling clays widely used in industry. Their mechanical properties are unequal according to their mineralogical and physico-chemical characteristics. The aim of this study is to improve the knowledge of the interlayer cation impact on the structure built by the smectite-water system according to the concentration. Homo-ionic (Na{sup +} et Ca{sup 2+}) and bi-ionic systems are observed. This study cross checks mineralogical methods, physicochemical analysis and broad range of rheometric tests. At low concentration (less than 60 g/l) the calcium dispersions are shear thinning and few viscous due to the layer association in huge deformable flocks. The sodium smectite layers are dispersed; the dispersions are highly viscous. The lowest viscosity is detected for mix of 20 % of sodium smectite and 80 % of calcium smectite. At higher concentration (60 to 100 g/l), the yield stress and viscoelastic properties are studied by creep-recovery tests, oscillatory tests and imposed shear step. At the liquid state, the flow is first heterogeneous with a shear banding effect then homogeneous. The results make it possible to define the concentration area characteristic of each mechanical behavior (viscosity, shear thinning and yield stress) according to the saturation cation. The thixotropic properties are characterized with de-structuring-restructuring tests. Two kinetics are determined. Finally we realize a data base with 12 natural and industrial bentonite. The rheograms would be efficient to differentiate the natural calcium bentonite (Newtonian law), natural sodium bentonite (Herschel-Bulkley law) and activated calcium bentonite (Bingham law). (author)

  7. Experimental study of the iron - smectite system in the presence of a solution at 80 at 300 deg; Etude experimentale du systeme fer-smectite en presence de solution a 80 deg et 300 deg

    Energy Technology Data Exchange (ETDEWEB)

    Guillaume, D


    One of the concepts proposed for the storage of nuclear wastes consists in the use of several confining barriers placed between the waste and the biosphere. Vitrified wastes are placed in steel containers surrounded by compacted smectite (engineered barrier). The whole is placed in the geological deep site (geological barrier, granite or argilite). The prediction of the evolution of the performances of the engineered barrier (low permeability, high swelling and sorption capacities) necessitates the study of the long-term stability of its mineral constituents under the conditions of the repository. The aim is to determine the nature and the chemistry of potential newly-formed phases resulting from the temperature increase due to disintegration reactions of the wastes, the presence of fluids coming from the geological barrier, and from the liberation of iron due to the degradation of the containers. Natural systems (in particular geothermal systems and sedimentary basins) give information concerning the possible evolutions of clay minerals. However, the potential availability of metallic iron makes a particular case of the nuclear waste storage. The aim of this experimental study is to put to evidence the chemical and mineralogical transformations of the iron-clay system at 80 deg C and 300 deg C, in the presence of a solution, so as to predict the possible transformations of a bentonite placed in the deep storage conditions of radioactive wastes. Several sets of experiments were carried out to study the reactivity of the MX80 bentonite in the presence of a solution ([CI]=3.10{sup -2} molal, Na/Ca=5,5 and liquid/rock ratio of 10) in absence or in presence of iron (magnetite + hematite or iron + magnetite) at 80 deg C or 300 deg C, and over periods ranging from 1 day to 9 months. Run products were submitted to multi-technique and multi-scale characterisation: X-ray diffraction, scanning electron microscopy, transmission electron microscopy (HR imaging, EDS and EELS

  8. Etude thermo-hydraulique de l'ecoulement du moderateur dans le reacteur CANDU-6 (United States)

    Mehdi Zadeh, Foad

    Etant donne la taille (6,0 m x 7,6 m) ainsi que le domaine multiplement connexe qui caracterisent la cuve des reacteurs CANDU-6 (380 canaux dans la cuve), la physique qui gouverne le comportement du fluide moderateur est encore mal connue de nos jours. L'echantillonnage de donnees dans un reacteur en fonction necessite d'apporter des changements a la configuration de la cuve du reacteur afin d'y inserer des sondes. De plus, la presence d'une zone intense de radiations empeche l'utilisation des capteurs courants d'echantillonnage. En consequence, l'ecoulement du moderateur doit necessairement etre etudie a l'aide d'un modele experimental ou d'un modele numerique. Pour ce qui est du modele experimental, la fabrication et la mise en fonction de telles installations coutent tres cher. De plus, les parametres de la mise a l'echelle du systeme pour fabriquer un modele experimental a l'echelle reduite sont en contradiction. En consequence, la modelisation numerique reste une alternative importante. Actuellement, l'industrie nucleaire utilise une approche numerique, dite de milieu poreux, qui approxime le domaine par un milieu continu ou le reseau des tubes est remplace par des resistances hydrauliques distribuees. Ce modele est capable de decrire les phenomenes macroscopiques de l'ecoulement, mais ne tient pas compte des effets locaux ayant un impact sur l'ecoulement global, tel que les distributions de temperatures et de vitesses a proximite des tubes ainsi que des instabilites hydrodynamiques. Dans le contexte de la surete nucleaire, on s'interesse aux effets locaux autour des tubes de calandre. En effet, des simulations faites par cette approche predisent que l'ecoulement peut prendre plusieurs configurations hydrodynamiques dont, pour certaines, l'ecoulement montre un comportement asymetrique au sein de la cuve. Ceci peut provoquer une ebullition du moderateur sur la paroi des canaux. Dans de telles conditions, le coefficient de reactivite peut varier de maniere



    Fabio, Maria Carolina; March, Samanta M.; Molina, Juan Carlos; Nizhnikov, Michael E.; Spear, Norman E.; Pautassi, Ricardo Marcos


    Prenatal ethanol exposure significantly increases later predisposition for alcohol intake, but the mechanisms associated with this phenomenon remain hypothetical. This study analyzed (Exp. 1) ethanol intake in adolescent inbred WKAH/Hok Wistar rats prenatally exposed to ethanol (2.0 g/kg) or vehicle, on gestational days 17–20. Subsequent Experiments (2, 3 and 4) tested several variables likely to underlie the effect of gestational ethanol on adolescent ethanol preference, including ethanol-in...

  10. Epigenetic regulation of Arc and c-Fos in the hippocampus after acute electroconvulsive stimulation in the rat

    DEFF Research Database (Denmark)

    Dyrvig, Mads; Hansen, Henrik H; Christiansen, Søren Hofman Oliveira


    Electroconvulsive stimulation (ECS) remains one of the most effective treatments of major depression. However, the underlying molecular changes still remain to be elucidated. Since ECS causes rapid and significant changes in gene expression we have looked at epigenetic regulation of two important...

  11. Interplay among Drosophila transcription factors Ets21c, Fos and Ftz-F1 drives JNK-mediated tumor malignancy

    Directory of Open Access Journals (Sweden)

    Eva Külshammer


    Full Text Available Cancer initiation and maintenance of the transformed cell state depend on altered cellular signaling and aberrant activities of transcription factors (TFs that drive pathological gene expression in response to cooperating genetic lesions. Deciphering the roles of interacting TFs is therefore central to understanding carcinogenesis and for designing cancer therapies. Here, we use an unbiased genomic approach to define a TF network that triggers an abnormal gene expression program promoting malignancy of clonal tumors, generated in Drosophila imaginal disc epithelium by gain of oncogenic Ras (RasV12 and loss of the tumor suppressor Scribble (scrib1. We show that malignant transformation of the rasV12scrib1 tumors requires TFs of distinct families, namely the bZIP protein Fos, the ETS-domain factor Ets21c and the nuclear receptor Ftz-F1, all acting downstream of Jun-N-terminal kinase (JNK. Depleting any of the three TFs improves viability of tumor-bearing larvae, and this positive effect can be enhanced further by their combined removal. Although both Fos and Ftz-F1 synergistically contribute to rasV12scrib1 tumor invasiveness, only Fos is required for JNK-induced differentiation defects and Matrix metalloprotease (MMP1 upregulation. In contrast, the Fos-dimerizing partner Jun is dispensable for JNK to exert its effects in rasV12scrib1 tumors. Interestingly, Ets21c and Ftz-F1 are transcriptionally induced in these tumors in a JNK- and Fos-dependent manner, thereby demonstrating a hierarchy within the tripartite TF network, with Fos acting as the most upstream JNK effector. Of the three TFs, only Ets21c can efficiently substitute for loss of polarity and cooperate with RasV12 in inducing malignant clones that, like rasV12scrib1 tumors, invade other tissues and overexpress MMP1 and the Drosophila insulin-like peptide 8 (Dilp8. While rasV12ets21c tumors require JNK for invasiveness, the JNK activity is dispensable for their growth. In conclusion, our study delineates both unique and overlapping functions of distinct TFs that cooperatively promote aberrant expression of target genes, leading to malignant tumor phenotypes.

  12. Gabapentin Differentially Modulate c-Fos Expression in Hypothalamus and Spinal Trigeminal Nucleus in Surgical Molar Extraction


    Kazi,Jamil Ahsan; Ibrahim,Ban Kahtan


    Abstract The study on the efficacy of oral analgesics reported that no single class of drug is effective in post-surgical dental pain. Pain following removal of third molar is most commonly used and widely accepted acute pain model for assessing the analgesic effect of drugs in humans. Reports demonstrated that analgesic efficacy in the human dental model is highly predictive. The high incidence of false-negative findings in analgesic investigations hinders the process of molecular discovery....

  13. Modeling of the climate system and of its response to a greenhouse effect increase; Modelisation du systeme climatique et de sa reponse a une augmentation de l'effet de serre

    Energy Technology Data Exchange (ETDEWEB)

    Li, L. [Universite Pierre et Marie Curie, CNRS, Lab. de Meteorologie Dynamique, 75 - Paris (France)


    The anthropic disturbance of the Earth's greenhouse effect is already visible and will enhance in the coming years or decades. In front of the rapidity and importance of the global warming effect, the socio-economical management of this change will rise problems and must be studied by the scientific community. At the modeling level, finding a direct strategy for the validation of climate models is not easy: many uncertainties exist because energy transformations take place at a low level and several processes take place at the same time. The variability observed at the seasonal, inter-annual or paleo- scales allows to validate the models at the process level but not the evolution of the whole system. The management of these uncertainties is an integral part of the global warming problem. Thus, several scenarios can be proposed and their risk of occurrence must be estimated. This paper presents first the greenhouse effect, the climatic changes during geologic times, the anthropic disturbance of the greenhouse effect, the modeling of climate and the forecasting of its evolution. (J.S.)

  14. The luteinising hormone surge-generating system is functional in male goats as in females: involvement of kisspeptin neurones in the medial preoptic area. (United States)

    Matsuda, F; Nakatsukasa, K; Suetomi, Y; Naniwa, Y; Ito, D; Inoue, N; Wakabayashi, Y; Okamura, H; Maeda, K-I; Uenoyama, Y; Tsukamura, H; Ohkura, S


    A luteinising hormone (LH) surge is fundamental to the induction of ovulation in mammalian females. The administration of a preovulatory level of oestrogen evokes an LH surge in ovariectomised females, whereas the response to oestrogen in castrated males differs among species; namely, the LH surge-generating system is sexually differentiated in some species (e.g. rodents and sheep) but not in others (e.g. primates). In the present study, we aimed to determine whether there is a functional LH surge-generating system in male goats, and whether hypothalamic kisspeptin neurones in male goats are involved in the regulation of surge-like LH secretion. By i.v. infusion of oestradiol (E2; 6 μg/h) for 16 h, a surge-like LH increase occurred in both castrated male and ovariectomised female goats, although the mean peak LH concentration was lower and the mean peak of the LH surge was later in males compared to females. Dual staining with KISS1 in situ hybridisation and c-Fos immunohistochemistry revealed that E2 treatment significantly increased c-Fos expression in the medial preoptic area (mPOA) KISS1 cells in castrated males, as well as ovariectomised females. By contrast, dual-labelled cells were scarcely detected in the arcuate nucleus (ARC) after E2 treatment in both sexes. These data suggest that kisspeptin neurones in the mPOA, but not those in the ARC, are involved in the induction of surge-like LH secretion in both male and female goats. In summary, our data show that the mechanism that initiates the LH surge in response to oestrogen, the mPOA kisspeptin neurones, is functional in male goats. Thus, sexual differentiation of the LH surge-generating system would not be applicable to goats. © 2014 British Society for Neuroendocrinology.

  15. Search for isotopic signatures of a supernova explosion close to the solar system in marine sediments; Recherche de signatures isotopiques dans les sediments marins de l'explosion d'une supernova proche du systeme solaire

    Energy Technology Data Exchange (ETDEWEB)

    Fitoussi, Caroline [Centre de Spectrometrie Nucleaire et de Spectrometrie de Masse, (CSNSM) IN2P3/CNRS, Campus d' Orsay, Bat 108, 91405 Orsay (France)


    The recent observation of a {sup 60}Fe peak in a deep-sea ferro-manganese crust has been interpreted as due to a supernova explosion relatively close to the solar system 2.8 {+-} 0.4 Myr ago. To confirm this interpretation with better time-resolved measurements, and the simultaneous access, on the same sample, to other isotopes and geochemical phases, marine sediments seem to be a tool of choice. The objective of this work was to search for isotopic anomalies which would be characteristic for residues of this supernova. More specifically, {sup 129}I, {sup 60}Fe, and {sup 26}Al have been investigated, being measured by Accelerator Mass Spectrometry (AMS). Quantifying these nuclides' fluxes would help constrain stellar nucleosynthesis models. These residues are isotopes initially produced during hydrostatic and/or explosive nucleosynthesis. The physical conditions during the explosion (temperature, neutron density) are such that supernovae are thought to be good candidates for the astrophysical site of the r-process. The {sup 129}I study showed that measurement of pre-anthropogenic {sup 129}I/{sup 127}I ratios need a very strict control of the various potential {sup 129}I sources, especially when working with small quantities (micrograms) of iodine. This study revealed that the expected pre-anthropogenic {sup 129}I/{sup 127}I ratio for pre-nuclear samples in the marine environment shows a large discrepancy between theoretical calculations and experimental measurements. {sup 60}Fe and {sup 26}Al measurements allow us to conclude that, in the authigenic phase of the marine sediments, there is no {sup 60}Fe anomaly in the time interval defined by the signal found on the Fe-Mn crust (from 2.4 to 3.2 Myr), and no {sup 26}Al anomaly from 2.6 to 3.2 Myr. (author)

  16. Acute Exposure to Fluoxetine Alters Aggressive Behavior of Zebrafish and Expression of Genes Involved in Serotonergic System Regulation

    Directory of Open Access Journals (Sweden)

    Michail Pavlidis


    Full Text Available Zebrafish, Danio rerio, is an emerging model organism in stress and neurobehavioral studies. In nature, the species forms shoals, yet when kept in pairs it exhibits an agonistic and anxiety-like behavior that leads to the establishment of dominant-subordinate relationships. Fluoxetine, a selective serotonin reuptake inhibitor, is used as an anxiolytic tool to alter aggressive behavior in several vertebrates and as an antidepressant drug in humans. Pairs of male zebrafish were held overnight to develop dominant—subordinate behavior, either treated or non-treated for 2 h with fluoxetine (5 mg L−1, and allowed to interact once more for 1 h. Behavior was recorded both prior and after fluoxetine administration. At the end of the experiment, trunk and brain samples were also taken for cortisol determination and mRNA expression studies, respectively. Fluoxetine treatment significantly affected zebrafish behavior and the expression levels of several genes, by decreasing offensive aggression in dominants and by eliminating freezing in the subordinates. There was no statistically significant difference in whole-trunk cortisol concentrations between dominant and subordinate fish, while fluoxetine treatment resulted in higher (P = 0.004 cortisol concentrations in both groups. There were statistically significant differences between dominant and subordinate fish in brain mRNA expression levels of genes involved in stress axis (gr, mr, neural activity (bdnf, c-fos, and the serotonergic system (htr2b, slc6a4b. The significant decrease in the offensive and defensive aggression following fluoxetine treatment was concomitant with a reversed pattern in c-fos expression levels. Overall, an acute administration of a selective serotonin reuptake inhibitor alters aggressive behavior in male zebrafish in association with changes in the neuroendocrine mediators of coping styles.

  17. Functions of the temporomandibular system in extracranial chronic pain conditions: modulatory effects on nocifensive behavior in an animal model. (United States)

    Fischer, Michael J; Stephan, Michael; Kielstein, Heike; Rahne, Henning; Nugraha, Boya; Gutenbrunner, Christoph; Ro, Jin Y; Svensson, Peter


    Mastication may be able to activate endogenous pain inhibitory mechanisms and therefore lead to modulation of nociceptive processing. The purpose of this study was to examine the possible effect of food consistency on noxious input from the spinal system. Three groups of adult male Sprague-Dawley rats were given an injection of complete Freund adjuvant in a hind paw 10 days after eating soft or hard food (one group received a saline injection-the control group [C]; the other group (D) received no injection). Nocifensive behavior was assessed with the use of the hot plate and tail flick assays at 1, 3, 6, and 12 hours and at 6.5 days after injection for groups A/B, and c-Fos activity was assessed in the brain after testing. Groups C/D had hot plate testing at 1 hour and 6.5 days. The data were analyzed by general linear modeling and 1-way analysis of variance. There was a small increase in the hot plate percent maximum possible effect (MPE) from -45.7 to -61.1 in group A over the length of the experiment, but a very small decrease for group B over the same period (-33.5 to -28.8). For the saline control group, there was a small increase toward 0 %MPE over the same time frame (-15.0 to 1.7). The %MPE differences were significant between groups A and C (P test. c-Fos was mainly observed in the raphe pallidus area with significant differences between groups A and B at 3 and 6 hours after injection of CFA (P = .027 and .022, respectively). The results of this study indicate that food consistency (hardness) influences nocifensive behavior in this animal model via a descending pathway operating at the supraspinal level. Copyright © 2014 National University of Health Sciences. Published by Elsevier Inc. All rights reserved.

  18. Effets de la microdose sur la production du niébé, du mil et du ...

    African Journals Online (AJOL)

    L'agriculture représente donc un secteur stratégique de développement du pays. Pendant longtemps, l'accroissement de production agricole s'est opéré à travers ..... management options on selected soil physical properties and sorghum yield. Int. J. Biol. Chem. Sci., 9(3): 1154-1170. v9i3.2.

  19. The Valve Gear Systems Timing Parameters Identification for Marine Diesel Engines Diagnostics / Identyfikacja Parametrów Układów Rozrządu Silników Okrętowych Dla Potrzeb Diagnostyki

    Directory of Open Access Journals (Sweden)

    Lus Tomasz


    Full Text Available W referacie przedstawiono wyniki badań, których celem było opracowanie metody diagnozowania okrętowych szybkoobrotowych silników tłokowych. Polska Marynarka Wojenna eksploatuje znaczną liczbę silników tego typu również na okrętach podwodnych. Silniki tego typu nie posiadają zaworów indykatorowych, co komplikuje możliwość oceny procesu spalania i ich stanu technicznego. Akademia Marynarki Wojennej w Gdyni od lat prowadzi badania związane z rozwojem metod diagnozowania okrętowych tłokowych silników spalinowych. W ostatnich latach prowadzone są prace nad metodami diagnozowania szybkoobrotowych silników okrętowych bazującymi na analizie obwiedni przyspieszeń drgań generowanych przez układy rozrządu zaworowego i układ paliwowy. W referacie zaprezentowano wybrane wyniki badań prowadzonych na silnikach Mercedes-Maybach typu MB820 stosowanych na okrętach podwodnych klasy Kobben.

  20. Accounting for uncertainties in real time hydrologic forecasting systems for the management of public dams in Quebec; Prise en compte des incertitudes dans la prevision hydrologique pour la gestion en temps reel des barrages publics du Quebec

    Energy Technology Data Exchange (ETDEWEB)

    Turcotte, R.; Lafleur, J.; Poirier, C.; Lacombe, P.; Brousseau, J. [Quebec Ministere du Developpement durable, de l' Environnement et des Parcs, Quebec, PQ (Canada). Centre d' Expertise Hydrique; Sparks, D.; Tapsoba, D. [Hydro-Quebec, Montreal, PQ (Canada)


    This paper presented details of new approaches to inflow forecast modelling developed by the Centre d'Expertise Hydrique du Quebec (CEHQ) as part its real-time decision-making management of Quebec public dams. The CEHQ now plans to include the impacts of quantitative precipitation and initial snow condition uncertainties within its program. In order to assess precipitation forecast uncertainties, distributions of errors using historical precipitation data and forecasted precipitation data were used. Conditional probabilities were then evaluated within the context of a special weather alert. A distributed hydrological model was used to simulate inflows that corresponded to various precipitation scenarios in order to determine a precipitation threshold which could be managed within dam outflow policies. The temporal evolution and spatial distribution of potential errors for basin snow water equivalent (SWE) were then studied separately. Outputs from a snow model were combined with tools from a geostatic model and used for error analysis. It was concluded that future research will focus on developing a strategy to incorporate SWE within the inflow forecasting model. 10 refs., 4 tabs., 5 figs.

  1. Présentation du volume

    Directory of Open Access Journals (Sweden)

    Ana Zwitter Vitez


    Full Text Available Dans de nombreuses disciplines scientifiques, le discours parlé représente depuis quelques décennies un objet de recherche proéminent. Ce fait s’explique probablement par deux caractéristiques principales du discours parlé: la structure complexe de sa matérialité et les fonctions variées de différentes pratiques langagières. C’est pourquoi le présent numéro réunit les articles basés sur les approches actuelles du discours parlé et sur les pratiques langagières spécifiques. Le volume est introduit par deux articles interdisciplinaires: Peter Garrard et Ahmed Samrah présentent le domaine de la pathologie du langage en donnant une revue fouillée sur les approches actuelles de l’analyse linguistique lors de la détection de la maladie d’Alzheimer, tandis que Harry Hollien donne un aperçu structuré sur le domaine de la phonétique judiciaire destiné à dévoiler l’identité des locuteurs et de détecter la déception dans le discours parlé. Les analyses explorant l’acquisition du langage commencent par l’article de Katharina Zipser qui examine la progression de structures grammaticales en les mettant en comparaison avec la compétence des apprenants et continuent par l’étude de Meta Lah proposant une évaluation des documents audiovisuels proposés aux apprenants de langue étrangère. Vesna Požgaj Hadži, Damir Horga et Tatjana Balažic Bulc remettent en question la corrélation entre la compétence linguistique et la fluence linguistique auprès de locuteurs non-maternels et l'analyse de Gemma Santiago Alonso aborde l'acquisition de l'article défini dans le langage enfantin. Les articles suivants se réunissent autour du domaine de l'interprétation: Jana Zidar Forte présente une approche actuelle dans l'entrainement d'interprètes, Lea Burjan analyse les phénomènes issus de la pratique de l'interprétation juridique et Simona  Šumrada traite de la reformulation dans le discours de la traduction et de

  2. les cahiers du cread - 2014

    African Journals Online (AJOL)

    Le systeme algerien de protection sociale : entre bismarckien et beveridgien · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Walid Merouani, Nacer-Eddine Hammouda, Claire El Moudden, 109-147 ...

  3. Le dispositif, un incontournable du moment

    Directory of Open Access Journals (Sweden)

    Françoise Demaizière


    Full Text Available 1.Introduction Je proposerai ci-après quelques réflexions à partir d'un terme qui est devenu omniprésent mais dont la définition et l'interprétation sont, me semble-t-il, assez variables. De telles variations sont courantes et ne sont pas forcément un obstacle majeur à l'action et à la réflexion. Il n'est toutefois pas inintéressant de s'arrêter un moment sur le ou les dispositifs d'apprentissage, de formation, multimédia, pédagogiques… 2.Omniprésence du terme, du mécanique au juridique et à ...

  4. Autobiographie, connaissance et implication du chercheur

    Directory of Open Access Journals (Sweden)

    Magali Humeau


    Full Text Available Ce texte propose de rapprocher les autobiographies des écrits de recherche scientifique. Si notre approche n'exclut pas le point de vue sociologique avec en particulier la recherche action, il se veut resserré ici sur l'approche bio-cognitive du sujet-chercheur avec le processus d'autoréférentiation, renvoyant à une épistémologie qui prend en compte et assume l'incomplétude propre à la connaissance. Toute production de savoir, y compris dans le cadre de la recherche scientifique, est ainsi conçue comme un jeu d'implication entre le sujet et son objet, mettant en œuvre nécessairement, mais pas toujours avec conscience, la référence à soi-même, processus permis dans le cadre du discours autobiographique.

  5. Chronologie du Yémen 2005

    Directory of Open Access Journals (Sweden)

    Laura Ruiz de Elvira


    Full Text Available Politique intérieureJanvier-Février1er janvier Vingt-cinq nouveaux juges sont désignés à la Cour suprême afin de résoudre les problèmes de corruption. 13 janvier Les locaux du journal en ligne al-Mu’tamar (dorénavant CPG, Congrès Populaire Général, ont été attaqués à la grenade pendant la nuit. Le cheikh ‘Abdul Mâjîd al-Zindânî est soupçonné.  30 janvierLes autorités yéménites interdisent aux Iraquiens résidant au Yémen de voter lors du scrutin destiné à élire les membres de l'Assemblée nati...

  6. Résidence du Corpus Troporum

    Directory of Open Access Journals (Sweden)

    Gunilla Iversen


    Full Text Available Les membres du Corpus Troporum, animé depuis des années par Gunilla Iversen (Stockholm, sont invités en résidence au Centre d’études médiévales d’Auxerre. Ce court séjour est destiné à mettre au point la publication du projet Sapientia- eloquentia. Il a été demandé au groupe d’organiser une journée sur les séquences et tropes de dédicace d’église (dimanche 23 octobre 2005.Presentation of the research project Corpus TroporumThe research project Corpus Troporum (formerly “Projektet Troper” h...

  7. L’écologie du coupable.

    Directory of Open Access Journals (Sweden)

    André Ourednik


    Full Text Available « Denn dass der Mensch erlöst werde von der Rache  : das ist mir die Brücke zur höchsten Hoffnung und ein Regenbogen nach langen Unwettern. » 1 Nous l’aurions presque oublié, en fouillant parmi les fondations du rapport moral au monde de nos sociétés, nous trouverons encore bien en place deux piliers érigés au cours du processus de la chrétienté : celui de l’ empathie et celui de la culpabilité . Le premier semble offrir le seul appui plausible à notre vivre ...

  8. Technostress et autres revers du travail nomade

    NARCIS (Netherlands)

    Popma, J.


    L'Internet et l'utilisation des ordinateurs portables, des téléphones mobiles et des tablettes ont accru l'importance du « travail nomade». Ce type de travail, sans contrainte de lieu ni de temps, peut entraîner, d’après ce working paper, une plus grande autonomie et une plus grande flexibilité pour

  9. Moyen Orient et Afrique du Nord

    International Development Research Centre (IDRC) Digital Library (Canada)

    à des produits chimiques toxiques… cela peut causer la mort si l'on n'administre pas rapidement l'antidote voulu. Depuis 1988, un système informatique appelé .... à passer de la culture du pavot à opium à celle de plantes à fort rapport économique comme le safran. □. Ouvrir la voie à la croissance des petites entreprises.

  10. Inventaire des champignons ectomycorhiziens du Maroc | Nounsi ...

    African Journals Online (AJOL)

    Objectif : Le travail présenté ici a pour objectif de dresser, à partir de la bibliographie, l'inventaire des espèces ectomycorhiziennes du Maroc. Méthodologie et résultats : Les données sur les champignons, recueillies à partir de différents travaux publiés nous ont permis de réaliser un premier catalogue concernant les ...

  11. Administration des subventions du CRDI aux institutions :

    International Development Research Centre (IDRC) Digital Library (Canada)


    Des transferts verticaux (entre les catégories budgétaires) peuvent être nécessaires en raison de l'inflation, d'une sous-estimation des coûts ou de modifications apportées sur le plan de la recherche. Il faut discuter de ces révisions avec l'administrateur de programmes compétent si elles dépassent 10 % du montant initial ...

  12. Retours, Circulations, Installations? Les Reconfigurations du ...

    African Journals Online (AJOL)

    Dans cet article – basé sur des données collectées à la fois à Ouagadougou et dans la province de la Comoé, (Burkina Faso) auprès des migrants de retour de Côte d'Ivoire ainsi qu'à bord du train reliant Ouagadougou à Abidjan – sont discutées les notions de retour et de circulation. Non seulement le retour ne débouche ...

  13. Autour du paradigme d’écotourisme

    Directory of Open Access Journals (Sweden)

    Anne-Laure Transler


    Full Text Available L’écotourisme ouvre un cadre conceptuel original qui permet d’envisager des démarches renouvelées de développement à partir des revenus issus de consommations touristiques mieux réfléchies et organisées, susceptibles de soutenir des initiatives de développement local s’inscrivant dans un projet global de développement durable. Variante douce du tourisme, loin du tourisme de masse, le champ de l’écotourisme s’étend aux confins du tourisme de nature, du tourisme culturel et des pratiques scientifiques et sportives de plein air. Une définition de l’écotourisme peut être proposée à partir des six points suivants : 1/ nature et culture, 2/ le bien-être des sociétés hôtes, 3/ des touristes responsables, 4/ la participation des sociétés hôtes, 5/ la durabilité, 6/ l’art de la rencontre.Ecotourism provides an original conceptual framework that enables approaches to development that are better able to provide a more equitable distribution of income, as well as better organized and thought through initiatives which are more likely to promote a holistic sustainable local development. With somewhat less negative impact than normal tourism, considerably less that mass tourism, ecotourism can encompass nature tourism, cultural tourism, scientific exploration, and even outdoor sports... A definition of ecotourism should take into account the following six points: 1/ nature and culture, 2/ the welfare of the host community, tourists acting responsibly, 3/ participation by the local community, 5 / sustainability , 6/ promotion of cultural exchanges

  14. Cri du Chat syndrome: a case report. (United States)

    Torres, Carolina Paes; Borsatto, Maria Cristina; de Queiroz, Alexandra Mussolino; Lessa, Fernanda Campos Rosetti; Orsi, Iara Agusta


    Cri du Chat Syndrome occurs as a result of a partial deletion in the short arm of chromosome 5. Among the consequent abnormalities are low birth weight, a striking catlike cry in infancy, mental retardation, epicanthal folds, hypertelorism and dental malocclusions. This paper presents a case report on the dental treatment of a 23-year-old patient who received multidisciplinary treatment, including special education and precocious stimulation for carriers of this syndrome.


    African Journals Online (AJOL)


    L'incidence du RYMV a été généralement faible, bien que les dégâts enregistrés dans certains points ... EPIDEMIOLOGY OF RICE YELLOW MOTTLE VIRUS DISEASE : DISTRIBUTION AND INCIDENCE ON CULTIVATED RICE. VARIETIES IN CÔTE D'IVOIRE .... au logiciel Excel. Les erreurs standar (ES) ont été estimées.

  16. Introduction du salariat dans les modes de

    African Journals Online (AJOL)

    sociétés gabonaises avant la pénétration coloniale. Cet univers s'élaborait selon un déterminisme socio«culturel propre à chaque groupe, et intériorisé .... même temps de l'autochtone un homme avide d'argent et de jouissance et non un ouvrier sûr et consciencieux (Schweitzer 1985:143). La nuance du vocabulaire relatif ...

  17. Communication interventriculaire post infarctus du myocarde ...

    African Journals Online (AJOL)

    Malgré la réduction importante de la mortalité des infarctus aigus durant ces dernières décennies grâce a une prise en charge médicale adéquate; monitoring cardiaque, une reperfusion précoce; le taux de mortalité suite à une rupture du septum interventriculaire (communication interventriculaire CIV) reste considérable.

  18. Thrombophlebite du sinus caverneux revelant une tuberculose ...

    African Journals Online (AJOL)

    Les auteurs rapportent une tuberculose maxillo-faciale chez un patient de 50 ans diabètique non insulino-requérrant révélée par une thrombophlébite du sinus caverneux responsable d'une exophtalmie bilatérale monstrueuse avec fonte purulente de l'oeil gauche et d'une parotidomégalie bilatérale réalisant une ...

  19. Les sens du musée

    Directory of Open Access Journals (Sweden)

    Laurent Grison


    Full Text Available Cet article, fondé sur l'exemple de la National Gallery de Londres, porte sur l'intégration dans le cadre urbain, l'aménagement et les dynamiques spatiales du lieu muséal. Sur le même thème, nous relatons un exercice réalisé avec des lycéens.

  20. Le profil entrepreneurial : Facteur discriminant du developpement ...

    African Journals Online (AJOL)

    A partir de la base de données GEM Algérie, nous montrons que le profil entrepreneurial, en tant qu'ensemble cohérent, est un bien meilleur discriminant du niveau de développement d'une économie que la part des exportations des biens minéraux dans l'exportation totale. Nous avons trouvé qu'il était possible ...

  1. Insectes ravageurs du piment Capsicum chinense Jacq ...

    African Journals Online (AJOL)


    30 sept. 2015 ... Mémoire Doctorat,. Université De La Réunion, 178p. Maisonneuve et Larose. 183-193. Makondy AER, 2012. Contrôle de la qualité des denrées alimentaires traitées avec les pesticides : cas de la tomate. Mémoire présenté et soutenu en vue de l'obtention du. Diplôme de Professeur de l'Enseignement.

  2. Mahmoud Zubeiri et la fondation du Parti de Dieu

    Directory of Open Access Journals (Sweden)

    Abdelmalek Attaieb


    Full Text Available A la veille de l’an 2000, au cœur des équilibres politiques du Yémen, perdure une union que symbolise bien la personnalité du Cheikh Abdallah al-Ahmar. A la fois leader du Rassemblement Yéménite pour la Réforme (al-Tagamu‘ al-Yemeni lil-Islah, la puissante formation islamiste créée en 1990 et chef de la confédération Hâched, le plus important des rassemblements tribaux du nord, le "Cheikh Abdallah", souvent considéré comme la seconde personnalité politique du pays, est également président du...

  3. Enfants du soleil histoire de nos origines

    CERN Document Server

    Brahic, André


    D'où venons-nous ? Comment le Soleil et la Terre sont-ils apparus ? Nous avons enfin atteint le moment où la science peut apporter des réponses de plus en plus précises, de plus en plus étonnantes. La Nature a décidément plus d'imagination que les hommes ! Depuis l'exploration spatiale, nous en avons plus appris sur notre système solaire et son origine en une génération qu'en quarante siècles. Là où l'on attendait l'uniformité, on a trouvé la plus grande diversité. André Brahic, qui a découvert les anneaux de Neptune, nous présente les multiples facettes du système solaire et nous raconte quatre milliards et demi d'années de son histoire. Les planètes sont les soeurs du Soleil. Nous sommes les enfants du temps et des étoiles. Sommes-nous seuls dans l'Univers ? Les astronomes commencent à apercevoir d'autres planètes autour de quelques soleils proches, Ils espèrent bientôt trouver d'autres Terres...

  4. La valorisation économique du bien-être animal : une application au cas du porc


    Latouche, Karine


    L'adoption des directives européennes imposant la prise en compte du bien-être des animaux dans les élevages pose la question de la valeur monétaire accordée à l'attribut respect du bien-être d'un produit animal donné sur le marché. La définition du bien-être animal retenue dans ce travail est celle imposée par la réglementation européenne, à savoir le respect des animaux selon certains critères imposés dans les différents textes, indépendamment de toute conséquence sur le produit final. D'ab...

  5. Violences du corps : Une étude du macabre chez Ronsard, Aubigné et Chassignet


    Aukrust, Kjerstin


    À la fin du seizième, début du dix-septième siècle, l’Europe est dominée par de nombreuses guerres, épidémies, famines et exécutions publiques. Couplé à un nouvel intérêt pour l’anatomie intérieure du corps humain, ce contexte historique sanglant crée une familiarisation avec le corps ouvert, avec la violence corporelle, la souffrance et la mort. Cette familiarisation est également percevable dans l’art sculptural et pictural de l’époque, ainsi que dans sa littérature, aussi bien sur la scène...

  6. La figure du roi et le point de vue du poète


    Buron, Emmanuel


    Parler des Discours de Jules Cesar avant le passage du Rubicon, d’Étienne Jodelle, dans le cadre d’un colloque sur César au Moyen Âge et à la Renaissance est à la fois évident et paradoxal. Évident, parce que le titre de ce poème indique d’emblée qu’il prend pour objet principal un épisode-clé de l’histoire du général romain. En outre, ce texte constitue la plus ambitieuse des entreprises poétiques du poète : selon Charles de la Mothe, qui préface les Œuvres et meslanges poetiques, édition de...

  7. Chapitre 6: Le cas de l'Afrique du Sud

    International Development Research Centre (IDRC) Digital Library (Canada)


    sud-africain, mais le pays ne verse que très peu de financement direct, si tant est qu'il en verse. Bien qu'ils ne ...... 17. 4. Un aperçu institutionnel de la recherche pour le développement en Amérique du Sud. En Afrique du Sud, la recherche pour le développement relève essentiellement du ministère des Sciences et de la ...

  8. Etude de la Pyrolyse en Analyse Thermogravimetrique du Carton ...

    African Journals Online (AJOL)

    La pyrolyse semble être un procédé adapté au traitement des déchets à base de carton. Afin d'optimiser les conditions expérimentales du procédé de pyrolyse et d'apporter des éléments pour le dimensionnement d'une installation, une étude du comportement thermique du carton est indispensable. Dans le présent ...

  9. lesions infra cliniques du sein a libreville (gabon): etude ...

    African Journals Online (AJOL)

    La prévalence du cancer du sein est en augmentation en Afrique. Les objectifs de cette étude étaient de déterminer la prévalence des lésions infra cliniques du sein diagnostiquées à Libreville (Gabon) et évaluer leur prise en charge. L'étude est rétrospective. Nous avons revu les dossiers des patientes opérées pour ...

  10. Kyste hydatique primitif du sein | Mouslik | Pan African Medical Journal

    African Journals Online (AJOL)

    Le kyste hydatique du sein est une parasitose rare même dans les pays endémiques. Nous rapportons une nouvelle observation d'une patiente de 30 ans qui présentait une masse du sein gauche. Le diagnostic de kyste hydatique du sein a été évoqué devant les données de l'examen clinique et de la mammographie ...

  11. Epidemiologie et Aspects Anatomo-Pathologique des Fractures du ...

    African Journals Online (AJOL)

    Objectifs : Déterminer l'incidence et les caractéristiques anatomopathologiques du fémur prise en charge dans le service de traumatologie orthopédie du CHU Tokoin de Lomé. Patients et Méthode : Il s'est agi d'une étude rétrospective fait sur les patients hospitalisés au service de traumatologie orthopédique du ...


    African Journals Online (AJOL)

    La prévalence du cancer du sein est en augmentation en Afrique. Les objectifs de cette étude étaient de déterminer la prévalence des lésions infra cliniques du sein diagnostiquées à Libreville (Gabon) et évaluer leur prise en charge. L'étude est rétrospective. Nous avons revu les dossiers des patientes opérées pour ...

  13. Utilisation du site Web | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Savoir. Innovation. Solutions. Carrières · Communiquez avec nous · Plan du site. Abonnez-vous à notre bulletin pour recevoir les nouvelles du CRDI chaque mois. Abonnez-vous · Droits d'auteur · Éthique de la recherche · Politique de libre accès · Politique de confidentialité · Transparence · Utilisation du site Web. Suivez- ...

  14. Le guide du consommateur sur la concurrence | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Savoir. Innovation. Solutions. Carrières · Communiquez avec nous · Plan du site. Abonnez-vous à notre bulletin pour recevoir les nouvelles du CRDI chaque mois. Abonnez-vous · Droits d'auteur · Éthique de la recherche · Politique de libre accès · Politique de confidentialité · Transparence · Utilisation du site Web. Suivez- ...

  15. Multiplication Rapide du Bananier Plantain ( Musa Spp. Aab) In Situ ...

    African Journals Online (AJOL)

    L'une des contraintes majeures limitant l'expansion et l'amélioration de la culture du bananier plantain est le manque de matériel végétal. La multiplication rapide du matériel de plantation nécessite le développement de techniques favorisant la production en masse de rejets. La technique de destruction du méristème ...


    African Journals Online (AJOL)

    LA FRACTURE DU CORPS CAVERNEUX. Tableau 1: Mécanisme de la Rupture Traumatique du Corps Caverneux. Mécanisme. Nombre de Cas Fréquence (%). Manipulation forcée de la verge en érection. Retournement sur vergé en érection pendant le sommeil. Faux pas du coi't. Choc sur verge en érection. Non précisé.

  17. Quelques observations sur les Bryopsis du Golfe de Naples

    NARCIS (Netherlands)

    Koster, Joséphine Th.


    Evidemment la flore algologique du Golfe de Naples a été étudiée, d’assez près, tant du point de vue floristique que du point de vue écologique. Surtout FALKENBERG (1879), BERTHOLD (1882) et FUNK (1927) se sont occupés des investigations de cette nature. Il reste cependant un certain nombre de

  18. Tribunal administratif de l'Organisation internationale du Travail (TAOIT)

    CERN Document Server

    International Labour Organization. Geneva

    Le Tribunal administratif de l'Organisation internationale du Travail connaît des requêtes formées par les fonctionnaires ou les anciens fonctionnaires du Bureau international du Travail et des quelque trente organisations qui ont reconnu sa compétence. The ILO Administrative Tribunal (ILOAT) hears complaints from serving and former officials of the International Labour Office or one of the thirty-odd intergovernmental organizations that recognise its jurisdiction.

  19. Augmentation du rendement des producteurs de petits mils par leur ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    cultiver la GPU 28 l'an prochain. Mme Amutha Puthur, agricultrice de Nammiyampattu, collines de Javadhu, Inde. Conclusion. Ces premières incidences du projet sont importantes tant sur le plan du produit (la détermination des variétés préférées des agriculteurs) que sur celui du processus (la SVP). La SVP fait en sorte ...

  20. impacts du changement climatique sur les ressources en eau en ...

    African Journals Online (AJOL)


    Les modèles de circulation générale GFD3 et UK89 ont été choisis parmi six modèles (GFD3, UK89, CCCM,. GISS, GF01 et CLIM) avec le modèle hydrologique (WATBAL) pour étudier les impacts du changement climatique sur les ressources en eau du bassin versant du Bandama. Les simulations prédisent en l'an.