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Sample records for bus bar splices

  1. The RRR of the Cu components of the LHC main bus bar splices

    CERN Document Server

    Heck, S; Fessia, P; Principe, R; CERN. Geneva. TE Department

    2010-01-01

    The different LHC main bus bar splice components (bus bar cable, splice U-piece and wedge and bus bar stabilizer) are subjected to different heat treatments (HTs) during bus bar assembly and splice soldering. The influence of soldering HTs on the RRR of the LHC main bus bar cable strands has been determined. The RRR of several splice U-pieces and wedges dismounted from the LHC has been measured. A correlation between the Vickers hardness and the RRR of the high purity Cu profiles has been established. All U-pieces tested that were produced before 2009 have a RRR>200, while the RRR of all wedges and of U-pieces of 2009 production have a much lower RRR. All tests of LHC main bus bar samples performed so far in the laboratory indicate a RRR of approximately 200 or higher.

  2. Resistance of LHC main bus bar splices at room temperature and at 77.4 K

    CERN Document Server

    Heck, S; Scheuerlein, Chr; CERN. Geneva. TE Department

    2009-01-01

    As part of the quality control the resistance of newly produced LHC main bus bar splices is now routinely measured at room temperature (RT) in order to conclude on the electrical continuity of the bus bar stabiliser across the splice under operating conditions. In this note we present splice resistance measurements that have been performed at RT and in liquid nitrogen (LN) in the CERN Cryolab with “ideal” splices (represented by continuous dipole and quadrupole bus bars), and with dipole and quadrupole splices with different defects, which cause an additional RT splice resistance of up to 60 µΩ. The RT resistance (RRT) results obtained with the Cryolab set-up are compared to the calculated resistance values and with the so-called R-8 and R-16 resistance results, as they are measured in the LHC tunnel with a Digital Low Resistance Ohmmeter with a voltage tap distance of 8 cm or 16 cm. The RT to LN resistance ratio has been determined for all splices in order to study the influence of the resistance of th...

  3. High quality joints of copper bus bars

    Directory of Open Access Journals (Sweden)

    Samarjit Bhattacharyya,

    2010-08-01

    Full Text Available Efficient joints in copper bus bar conductors can be made very simply by bolting and clamping. This paper proposes the method to maximize the jointing efficiency in order to eliminate hot spots in switchgears by optimizing the effect of spreading resistance and interface resistance.

  4. A train for the bus(bars)

    CERN Multimedia

    CERN Bulletin

    2013-01-01

    On 8 April, the first SMACC (Superconducting Magnets and Circuits Consolidation) teams began work in the LHC tunnel. They are responsible for opening the interconnects between the magnets, laying the groundwork for the series of operations that will be performed by the team riding the ‘consolidation train’.   A technician installs the machine tool that allows them to prepare the surface of the section of the bar where the shunt will be fixed. The LHC’s 1,670 dipoles and quadrupoles are powered by power converters and connected by copper 'busbars’. The superconducting cables run through these bars, carrying a current of up to 11,850 amps. Six superconducting cables meet at each interconnect, where they are held together by a soldered (see box) electrical connection sandwiched between two pieces of copper, forming the splice between the busbars of the neighbouring magnets. The integrity of the electrical circuit is depen...

  5. Bus bar electrical feedthrough for electrorefiner system

    Science.gov (United States)

    Williamson, Mark; Wiedmeyer, Stanley G; Willit, James L; Barnes, Laurel A; Blaskovitz, Robert J

    2013-12-03

    A bus bar electrical feedthrough for an electrorefiner system may include a retaining plate, electrical isolator, and/or contact block. The retaining plate may include a central opening. The electrical isolator may include a top portion, a base portion, and a slot extending through the top and base portions. The top portion of the electrical isolator may be configured to extend through the central opening of the retaining plate. The contact block may include an upper section, a lower section, and a ridge separating the upper and lower sections. The upper section of the contact block may be configured to extend through the slot of the electrical isolator and the central opening of the retaining plate. Accordingly, relatively high electrical currents may be transferred into a glovebox or hot-cell facility at a relatively low cost and higher amperage capacity without sacrificing atmosphere integrity.

  6. DWPF Melter No.2 Prototype Bus Bar Test Report

    International Nuclear Information System (INIS)

    Characterization and performance testing of a prototype DWPF Melter No.2 Dome Heater Bus Bar are described. The prototype bus bar was designed to address the design features of the existing system which may have contributed to water leaks on Melter No.1. Performance testing of the prototype revealed significant improvement over the existing design in reduction of both bus bar and heater connection maximum temperature, while characterization revealed a few minor design and manufacturing flaws in the bar. The prototype is recommended as an improvement over the existing design. Recommendations are also made in the area of quality control to ensure that critical design requirements are met

  7. Constant of heat conduction and stabilization of bus bar conductor

    Science.gov (United States)

    López, G.

    Using the one-dimensional, time-independent conduction state, a constant of heat conduction is given bringing about the known stabilization theorem and a closed expression for the bus bar to be cryogenically stable in superconducting accelerators.

  8. Black silicon with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io;

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by mask-less reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Black bus-bars were realized by oxidized copper resulting in reflectance below 3% in the entire visible wavelength range. The combination of these two technologies may result in aesthetic, all-black panels based on conventional, front-contacted solar cells...

  9. Bus bar joints stability and protection

    CERN Document Server

    Verweij, A

    2009-01-01

    A defective bus connection between two dipole magnets was the primary cause of the incident in sector 34 on September 19th. I will show how this could have happened, i.e. how a highly resistive joint has caused a thermal runaway and burned (or opened) before the QPS threshold was reached. In the second part of the talk I will present the new detection limits for the QPS upgrade of the RB and RQ circuits, required to avoid similar thermal runaways in the future.

  10. Loss of power of a 220 VAC safety bus bar at Embalse NPP

    International Nuclear Information System (INIS)

    Embalse nuclear power plant is located at the Cordoba Province, 5 kilometres Southern the town of Embalse. It is equipped with one 600 MWe CANDU reactor. At the time of the event, which occurred on 11 October 2004, the plant was operating normally at full power. The Embalse nuclear power plant electrical power system buses are classified, in reliability decreasing order, into the following four different levels: 1) Class I: uninterrupted direct current supplies for safety related and other essential loads; 2) Class II: uninterrupted alternating current supplies for safety related and other essential loads; 3) Class III: alternating current supplies to essential auxiliaries which can tolerate the short interruption required until to start up and load the on-site standby generators and 4) Class IV: normal alternating current supplies to auxiliaries which can tolerate long duration interruptions without affecting personnel or equipment safety (Class IV is the normal source of power to Class III system). In particular, the Class II 220 VAC uninterrupted monophasic safety bus bars are composed by three 220 VAC subsystems powered by the following two sources of power: Class I uninterrupted safety bus bars (5551 BUA, BUB and BUC bars) and Class III secondary distribution 5433-MCC5 and MCC6. The Class II 220 VAC bus bars are normally powered from Class I bus bars through static monophasic inverters. Beside, in a static monophasic out of service case, Class II bus bar affected could be powered from Class III bus bars via a transformer stabilizer. Class II bus bars supplies power to both control computers and the AC instrumentation and control devices. When 220 VAC (Class II) 5542-BUC-monophasic-inverter failure was coincident with a subsequent independent failure to transfer to the Class III through static commuter 5542-SVR-C, a loss of the 220 VAC uninterrupted monophasic safety bus bar (Class II) event was caused. (author)

  11. Calculation of Flexible Bus-Bars Electrodynamic Stability with Application of Implicit Scheme

    OpenAIRE

    Y. G. Panamarenka

    2008-01-01

    A numerical method for calculation of open-air substations’ flexible bus-bars dynamic at short-circuit has been improved on equations of a flexible elastic string with application of an implicit scheme. On the basis of the numerical method a computer program FLEBUS for calculation of substations’ flexible bus-bars dynamic at short-circuit has been developed. An approbation and an estimation of calculation result reliability have been carried out in accordance with the program while using expe...

  12. Initial magnetic field distribution around high rectangular bus bars

    OpenAIRE

    Cividjian Grigore A.

    2014-01-01

    The one-dimensional transient electromagnetic field in and around a system of two nonmagnetic homogenous rectangular high thin bars can be analytically evaluated if the ratio of average initial magnetic field on the two sides of thin bar, or of the ratio of initial magnetic fields in middle of the bar height is known. In this paper, using appropriate conformal mappings, an exact analytical solution for these ratios are proposed in the case of very thin bars...

  13. Initial magnetic field distribution around high rectangular bus bars

    Directory of Open Access Journals (Sweden)

    Cividjian Grigore A.

    2014-01-01

    Full Text Available The one-dimensional transient electromagnetic field in and around a system of two nonmagnetic homogenous rectangular high thin bars can be analytically evaluated if the ratio of average initial magnetic field on the two sides of thin bar, or of the ratio of initial magnetic fields in middle of the bar height is known. In this paper, using appropriate conformal mappings, an exact analytical solution for these ratios are proposed in the case of very thin bars. Obtained values are compared with FEM results for relatively thick bars.

  14. Calculation of Flexible Bus-Bars Electrodynamic Stability with Application of Implicit Scheme

    Directory of Open Access Journals (Sweden)

    Y. G. Panamarenka

    2008-01-01

    Full Text Available A numerical method for calculation of open-air substations’ flexible bus-bars dynamic at short-circuit has been improved on equations of a flexible elastic string with application of an implicit scheme. On the basis of the numerical method a computer program FLEBUS for calculation of substations’ flexible bus-bars dynamic at short-circuit has been developed. An approbation and an estimation of calculation result reliability have been carried out in accordance with the program while using experimental data. On the basis of the obtained information it is possible to assert that the developed program is an independent tool for calculation of electrodynamic stability of substations’ flexible bus-bars.

  15. Implementation of Bus Bar Switching and Short Circuit Constraints in Optimal Power Flow Problems

    OpenAIRE

    Van Acker, Tom; Van Hertem, Dirk; Bekaert, David; Karoui, Karim; Merckx, Christian

    2015-01-01

    The topology of an electric network heavily influences power system operations, power flows, short circuit currents and certain reliability aspects. While meshing increases reliability, it also increases the short circuit power, which can bea problem especially in highly meshed grids. Bus bar switchingis often employed by the system operator for preventive andcorrective actions, as it is a quasi-free control action. Theintroduction of binary variables, representative of the state ofthe circui...

  16. Measurement of Parasitic Inductances in the Bus-Bar Assembly of a High Power Voltage Source Converter

    Science.gov (United States)

    Datta, Aniket; Narayanan, G.

    2016-05-01

    Insulated gate bipolar transistor (IGBT) based voltage source converters use copper plates with insulating sheets in between them (sandwich bus-bar arrangement) for connecting the different device terminals in the power circuit. In such converters, the parasitic inductances in the power circuit are crucial as they cause overvoltage spikes across the device. Also, the parasitics affect the current sharing between IGBTs when they are connected in parallel in high power converters. The conduction path through plates and fasteners in the bus-bar assembly is three-dimensional and quite complex, making analytical evaluation of the stray inductance quite challenging. The first objective here is to present a simple experimental setup and experimental procedure, which are convenient for power electronic engineers, to measure the bus-bar inductance. The next objective is to carry out experimental studies on the inductances offered by different components and sub-assemblies in a bus-bar assembly. This includes evaluation of inductances of the different conduction paths in typical bus-bar plates. The third objective is to experimentally evaluate the parasitic inductances in the bus-bar assembly of a commercial 250 kVA high power converter. Each leg of this converter consists of two 300 A/1200 V IGBTs connected in parallel. The effective inductance seen by the individual device modules are determined experimentally.

  17. Effect of extrusion wheel angular velocity on continuous extrusion forming process of copper concave bus bar

    Institute of Scientific and Technical Information of China (English)

    WU Peng-yue; XIE Shui-sheng; LI Hua-qing; YAN Ming; HUANG Guo-jie; CHENG Lei

    2007-01-01

    The continuous extrusion forming process for producing large section copper concave bus bar under different extrusion wheel angular velocities was studied by three-dimensional finite element technology based on software DEFORM-3D. The rigid-viscoplastic constitutive equation was employed in the model. The numerical simulation results show that the deformation body flow velocity in the die orifice increases gradually with the increase of the extrusion wheel angular velocity. But slippage between the rod and extrusion wheel occurs when the extrusion wheel angular velocity is high. The effective stress near the die orifice enhances gradually with increasing extrusion wheel angular velocity. High stress is concentrated in adjacent regions of the flash gap. The effective strain gradient is greater near the abutment than that near the die orifice. The effective strain of the product increases gradually with increasing extrusion wheel angular velocity. In the deformation process, the deformation body temperature increases remarkably due to friction and deformation. So the cooling is necessary in the region of the die and tools.

  18. High-Current Bus Splice Resistances and Implications for the Operating Energy of the LHC

    CERN Document Server

    Koratzinos, M; Charifoulline, Z; Dahlerup-Petersen, K; Denz, R; Flora, R H; Pfeffer, H; Scheuerlein, C; Schmidt, R; Siemko, A; Strait, J; Verweij, A

    2010-01-01

    At each interconnection between LHC main magnets a low-resistance solder joint must be made between superconducting cables in order to provide a continuous current path through the superconductor and also to the surrounding copper stabilizer in case the cable quenches [1]. About 10,000 such joints exist in the LHC. An extensive campaign has been undertaken to characterize and map the resistances of these joints. All of the superconducting cable splices were measured at 1.9 K and no splices were found with a resistance larger than 3 nW. Non-invasive measurements of the stabilizer joints were made at 300 K in 5 of the 8 sectors, and at 80 K in 3 sectors. More precise local measurements were made on suspect interconnects that were opened up, and poor joints were repaired. However, it is likely that additional imperfect stabilizer joints still exist in the LHC. A statistical analysis is used to place bounds on the remaining worst-case resistances. This sets limits on the maximum operating energy of the LHC, prior...

  19. Discussion on microprocessor-based bus bar protection%浅谈母差保护微机化的几点感受

    Institute of Scientific and Technical Information of China (English)

    舒逸石; 马勇; 于培杰

    2009-01-01

    通过总结大量变电站母差保护微机化改造中发现的问题,从电流互感器、二次负载、抗饱和能力、保护动作时间、运行方式判别等几个方面,对继电器式和微机式母差保护进行了对比分析,指出工程人员往往只重视保护的更换,而忽略了电流互感器的更换,导致因电流互感器与保护不匹配而产生母差保护误动作,同时阐明了微机式母差保护的优点.%Through a large number of problems about rebuilding transformer substation microprocessor bus bar protection, this paper analyzes several aspects of relayed bus bar protection and microprocessor-based bus bar protection from current transformers, the secondary load, the anti-saturation ability, the action time and operating mode. Then it points out that engineers often pay more attention on the replacement of protection, neglects the replacement of current transformers, which leads to the mistaken action of bus bar protection because current transformer is mismatched with protection. At the same time, it illustrates the advantages of microprocessor-based bus bar protection.

  20. Current lead and bus bar system for the 1.8 K test of the EURATOM LCT-coil

    International Nuclear Information System (INIS)

    For the test of EURATOM LCT coil at 1.8 K in the TOSKA facility, it is foreseen to use the 23 kA current leds developed for the POLO model coil. Special emphasis has been given to the connection of the current leads which will operate by using supercritical helium at 4.2 K to the coil terminals which are on the 1.8 K level. The heat input from the bus bar system (4.2 K) to the coil winding cooling circiut (1.8 K) for each terminal is only in the range of 10 to 15 W. The calculations show that the current leads together with the superconducting connection bars are safe: They can withstand a loss of mass flow for more than one minute withonly a very small increase of heat load into the coil if its cooling circuit stays in operation. (orig.)

  1. Measurement of the Residual Resistivity Ratio of the Bus Bars Copper Stabilizer of the 13 kA Circuits of the LHC

    CERN Document Server

    Apollonio, A; Solfaroli Camillocci, M; Schmidt, R; Verweij, A; Siemko, A; Claudet, S; Steckert, J; Thiesen, H

    2012-01-01

    After the incident of September 2008, the operational beam energy of the LHC has been set to 3.5 TeV, since not all joints of the superconducting (SC) bus bars between magnets have the required quality for 7 TeV operation. This decision is based on simulations to determine the safe current in the main dipole and quadrupole circuits, reproducing the thermal behaviour of a quenched superconducting joint by taking into account all relevant factors that affect a possible thermal runaway. One important parameter is the Residual Resistivity Ratio (RRR) of the copper stabilizer of the bus bar connecting the superconducting magnets. A dedicated campaign to measure the RRR for the main 13 kA circuits of the LHC in all sectors was performed during the Christmas stop in December 2010 and January 2011. The measurement method as well as the data analysis and results are presented in this paper.

  2. Copper Heat Exchanger for the External Auxiliary Bus-Bars Routing Line in the LHC Insertion Regions

    CERN Document Server

    Garion, C; Seyvet, F; Sitko, M; Skoczen, B; Tock, J P

    2006-01-01

    The corrector magnets and the main quadrupoles of the LHC dispersion suppressors are powered by a special superconducting line (called auxiliary bus-bars line N), external to the cold mass and housed in a 50 mm diameter stainless steel tube fixed to the cold mass. As the line is periodically connected to the cold mass, the same gaseous and liquid helium cools both the magnets and the line. The final sub-cooling process (from around 4.5 K down to 1.9 K) consists in the phase transformation from liquid to superfluid helium. Heat is extracted from the line through the magnets via their point of junction. In dispersion suppressor zones, approximately 40 m long, the sub-cooling of the line is slightly delayed with respect to the magnets. This might have an impact on the readiness of the accelerator for operation. In order to accelerate the process, a special heat exchanger has been designed. It is located in the middle of the dispersion suppressor portion of the line. Its main function consists in providing a loca...

  3. Design and analysis of bus-bar of marine main switchboard%船舶主配电板主汇流排设计与分析

    Institute of Scientific and Technical Information of China (English)

    冒如权; 郭建; 方雄伟

    2014-01-01

    The most important part of marine power distribution system is main switchboard, and its key part is main bus-bar, which affects safety and reliability of the power distribution system. This paper describes the design method of the main bus-bar of marine main switchboard, mainly including its selection and veriifcation and analysis of its thermal and dynamic stability combined with calculation of short circuit current. It also proposes the relevant veriifcation calculation equations and introduces the practical application of a case of design of main switchboard with three rectangular copper bus-bar installed in parallel.%主配电板为船舶电力配电系统的重要组成部分,主汇流排作为主配电板的核心部分,关系到电力配电系统的安全可靠运行。文中阐述了船舶主配电板主汇流排的设计方法,主要包括船舶主配电板主汇流排规格选择,以及结合短路电流计算对汇流排进行的热稳定和动稳定校验分析,并给出相关校核计算公式;最后通过三根矩形铜排并联使用的主配电板设计实例介绍计算方法的具体应用。

  4. Stabilizer bar design about the rear suspension of a light bus%某轻型客车后悬架稳定杆设计

    Institute of Scientific and Technical Information of China (English)

    戴声良; 唐学东; 王长文; 李磊; 刘辉

    2009-01-01

    A stabilizer bar was designed based on one light bus single tilted arm rear suspension. Motion harmony and validity in suspension system are analyzed according to geometry relationship. The experiment results show that steering stability of the full vehicle is improved by the stabilizer bar, and the rolling angle stiffness between front and rear suspension matches more reasonably.%对某轻型客车单斜臂式后悬架布置横向稳定杆,根据几何关系分析了横向稳定杆在悬架系统中运动协调性和有效性;试验结果表明,设计的稳定杆能有效改善整车的操纵稳定性,前后悬架的侧倾角刚度匹配也更趋合理.

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  20. ELECTRO-THERMAL AND MECHANICAL VALIDATION EXPERIMENT ON THE LHC MAIN BUSBAR SPLICE CONSOLIDATION

    CERN Document Server

    Willering, GP; Bourcey, N; Bottura, L; Charrondiere, M; Cerqueira Bastos, M; Deferne, G; Dib, G; Giloux, Chr; Grand-Clement, L; Heck, S; Hudson, G; Kudryavtsev, D; Perret, P; Pozzobon, M; Prin, H; Scheuerlein, Chr; Rijllart, A; Triquet, S; Verweij, AP

    2012-01-01

    To eliminate the risk of thermal runaways in LHC interconnections a consolidation by placing shunts on the main bus bar interconnections is proposed by the Task Force Splices Consolidation. To validate the design two special SSS magnet spares are placed on a test bench in SM-18 to measure the interconnection in between with conditions as close as possible to the LHC conditions. Two dipole interconnections are instrumented and prepared with worst-case-conditions to study the thermo-electric stability limits. Two quadrupole interconnections are instrumented and prepared for studying the effect of current cycling on the mechanical stability of the consolidation design. All 4 shunted interconnections showed very stable behaviour, well beyond the LHC design current cycle.

  1. BUS 210 Course tutorial/ indigohelp

    OpenAIRE

    WVFD

    2015-01-01

    For more classes visit www.indigohelp.com   BUS 210 Week 1 DQ1 and DQ2 BUS 210 Week 1 Checkpoint Business Models and Systems (2 Sets) BUS 210 Week 1 Knowledge Check BUS 210 Week 2 CheckPoint Business Organization  (2 Sets) BUS 210 Week 2 Knowledge Check BUS 210 Week 2 Assignment Evolution of Business Presentation (2 Sets) BUS 210 Week 3 DQ 1 and DQ 2 BUS 210 Week 3 CheckPoint Code of Ethics (2 Sets) BUS 210 Week 3 Knowledge Check BUS 2...

  2. BUS 235 Course tutorial/ indigohelp

    OpenAIRE

    SFVD

    2015-01-01

    For more classes visit www.indigohelp.com   BUS 235 Week 1 DQ 1 Marketing Trends BUS 235 Week 1 DQ 2 Customer Needs and Wants BUS 235 Week 2 DQ 1 Market Research BUS 235 Week 2 DQ 2 Customer Loyalty BUS 235 Week 3 DQ 1 Branding Debate BUS 235 Week 3 DQ 2 New Product Development BUS 235 Week 3 Assignment Retail Store (Walmart) BUS 235 Week 4 DQ 1 Price and Ethics BUS 235 Week 4 DQ 2 Service Marketing BUS 235 Week 5 DQ 1 Super Bowl Advertisin...

  3. PCI bus demystified

    CERN Document Server

    Abbott, Doug

    2004-01-01

    The peripheral component interconnect (PCI) bus is the dominant bus system used to connect the different elements making up today''s high-performance computer systems. Different PCI implementations have also been developed for such applications as telecommunications and embedded computing. If an application calls for high speed, high reliability, flexible configuration, and bus mastering, then PCI is the only logical bus choice. This book is an applications-oriented introduction to the PCI bus, with an emphasis on implementing PCI in a variety of computer architectures. Special attention is given to industrial and mission-critical applications of PCI bus.·Fully describes PCI electrical specifications, mechanical requirements, and signal types·Covers advanced topics through numerous design examples to increase the readers understanding of the subject ·Includes updated coverage of PCI-X 2.0

  4. Intelligent Bus Stops in the Flexible Bus Systems

    Directory of Open Access Journals (Sweden)

    Razi Iqbal

    2014-09-01

    Full Text Available The purpose of this paper is to discuss Intelligent Bus Stops in a special Demand Responsive Transit (DRT, the Flexible Bus System. These Intelligent Bus Stops are more efficient and information rich than Traditional Bus Stops. The real time synchronization of the Flexible Bus System makes it unique as compared to Traditional Bus Systems. The Main concern is to make Bus Stops intelligent and information rich. Buses are informed about the no. of passengers waiting at the upcoming Bus Stops. If there are no passengers to ride or get off on upcoming Bus Stop, the Bus can skip that Bus Stop and head towards the next Bus Stop where passenger is waiting, which will decrease the ride time of the passengers on the Bus and also the wait time of the passengers waiting on the upcoming Bus Stops. Providing more information at Bus Stops about the Destination (Time to Destination, Distance to Destination etc. and Buses (Bus Location, Arrival Time of Bus etc. makes it easier for the passengers to decide whether to ride a particular Bus or not.

  5. Splicing Programs and Cancer

    OpenAIRE

    Sophie Germann; Lise Gratadou; Martin Dutertre; Didier Auboeuf

    2012-01-01

    Numerous studies report splicing alterations in a multitude of cancers by using gene-by-gene analysis. However, understanding of the role of alternative splicing in cancer is now reaching a new level, thanks to the use of novel technologies allowing the analysis of splicing at a large-scale level. Genome-wide analyses of alternative splicing...

  6. Parameterizable Wishbone Bus

    OpenAIRE

    Hussain Fawzi, Omar; Alagedi, Alfiqar

    2012-01-01

    In the industry of intellectual property products "IP-cores", a communication link is almost always needed. A semiconductor intellectual property IP core is a reusable unit of logic in electronic design. IP cores are used as building blocks for ASIC chip design or FPGA logic designs. A bus creates a communication link between the IP cores in a system. The company AnaCatum Design AB have many projects where a bus is needed. Creating a new bus structure for every project is time consuming. By h...

  7. The erigone bus study

    International Nuclear Information System (INIS)

    A study has been made of some factors determining the performance and cost-effectiveness of microcomputer bus systems implemented in Europa crates. An experimental system has been specified and a number of modules constructed in accordance with the specification, in order to test the validity of some of the ideas proposed. It has been demonstrated that the bus interfacing logic can be simple and inexpensive, while maintaining a high degree of processor independence and also a high degree of transparency in operation. The number of pins required by the bus is minimized by multiplexing address and data. A series termination for the drive of the multiplexed lines permits cross-talk to be reduced to insignificant levels: this technique is recommended for wider application. The experimental bus is proposed as a basis for the construction of simple microcomputer-based instrumentation systems. (orig.)

  8. Bus.py

    Energy Technology Data Exchange (ETDEWEB)

    2014-07-21

    GridLAB-D is an agent? based distribution system simulation environment that allows fine-grained end-user models, including geospatial and network topology detail. GridLAB-D addresses the lack of runtime interaction by designing a flexible communication interface, Bus.py (pronounced bus-dot-pie), that uses Python to pass messages between one or more GridLAB-D instances and a Smart Grid simulator.

  9. LHC on the bus

    CERN Multimedia

    Laëtitia Pedroso

    2010-01-01

    On 15 December, an airport bus will be transformed in the image of CERN. The bus will be seen by the thousands of travellers arriving in Geneva, informing them of the possibility to visit CERN.   Sketch of the bus. The good relationship between Geneva International Airport and CERN started several years ago. In 2004 the airport put advertising space in the arrivals area at CERN's disposal free of charge. Now, starting on 15 December, a 40-foot long bus will display a giant sticker advertisement depicting CERN as it takes passengers over the airport tarmac to their planes. This is no ordinary sticker, and it was no mean task to attach it to the bus. The task of producing and attaching it was entrusted to Geneva-based specialists Mathys SA. With the ski season opening on 15 December, there will be many travellers arriving at the airport, and the bus will be ready to receive them. When one thinks of CERN, the subjects that naturally come to mind are the LHC, the mysteries of the Universe...

  10. Savannah River bus project

    Energy Technology Data Exchange (ETDEWEB)

    Summers, W.A. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1998-08-01

    The H2Fuel Bus is the world`s first hybrid hydrogen electric transit bus. It was developed through a public/private partnership involving several leading technology and industrial organizations in the Southeast, with primary funding and program management provided by the Department of Energy. The primary goals of the project are to gain valuable information on the technical readiness and economic viability of hydrogen buses and to enhance the public awareness and acceptance of emerging hydrogen technologies. The bus has been operated by the transit agency in Augusta, Georgia since April, 1997. It employs a hybrid IC engine/battery/electric drive system, with onboard hydrogen fuel storage based on the use of metal hydrides. Initial operating results have demonstrated an overall energy efficiency (miles per Btu) of twice that of a similar diesel-fueled bus and an operating range twice that of an all-battery powered electric bus. Tailpipe emissions are negligible, with NOx less than 0.2 ppm. Permitting, liability and insurance issues were addressed on the basis of extensive risk assessment and safety analyses, with the inherent safety characteristic of metal hydride storage playing a major role in minimizing these concerns. Future plans for the bus include continued transit operation and use as a national testbed, with potential modifications to demonstrate other hydrogen technologies, including fuel cells.

  11. BUS 660 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

      www.uophelp.com         BUS 660 Week 1 Assignment Developing Leaders at UPS BUS 660 Week 2 Assignment Leadership Style BUS 660 Week 3 Assignment Leadership Traits BUS 660 Week 4 Assignment Leadership Skills BUS 660 Week 5 Assignment Big Changes for a Small Hospital BUS 660 Week 6 Assignment Research Paper BUS 660 Week 1 DQ 1 Leadership Defined BUS 660 Week 1 DQ 2 Education and Experience BUS 660 Week 2 DQ 1 Lea...

  12. A study of lapped splices in reinforced concrete columns under severe cyclic loads

    Science.gov (United States)

    Lukose, K.; Gergely, P.; White, R. N.

    1981-07-01

    In an investigation of the behavior of overlapped reinforcing rods under high level, inelastic, reversing cyclic loads, 14 tests were conducted on column specimens with No. 6 spliced bars at the corners of surrounding No. 3 stirrups, subjected to combined bending and shear. The relationship between the splice length and the stirrup spacing was studied in detail. The most significant result is that a reasonable level of ductility in splices under combined bending and shear was achieved by providing uniformly spaced stirrups along the splice, and closely spaced stirrups just outside the high moment splice end. An equation for splice design was developed for specimens of the type tested. Experimental results are discussed in terms of load versus displacement, energy absorption, stiffness reduction, main bar strain variation, compression splice behavior and bond-shear interaction.

  13. BusTechn

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Introduced the characteristics of series communicat io n I 2C bus and basic communication protocol, and introduced the application of the bus I 2C according to the image sensor OV7610.The computer correspondence s tyle can be divided the parallel correspondence and the series correspondence. T he rate of series correspondence is rapid, and the property of real time is exce llent. Because the number of ports which it takes is great, it is not proper to miniaturize. Whereas the rate of series corresponden...

  14. BUS 644 (New) Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    www.uophelp.com       BUS 644 Week 1 DQ 1 Ethical Issues and Operations BUS 644 Week 1 DQ 2 Business Processes BUS 644 Week 1 Midas Case Study BUS 644 Week 2 DQ 1 Productivity BUS 644 Week 2 DQ 2 Product Design and Quality BUS 644 Week 2 Memorial Hospital Case Study BUS 644 Week 3 DQ 1 Suppliers BUS 644 Week 3 DQ 2 Forecasting Methods BUS 644 Week 3 Outsourcing BUS 644 Week 4 DQ 1 Process Selection Product Design and Capacity BUS 64...

  15. BUS 370 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

     For more course tutorials visit www.uophelp.com   BUS 370 Week 1 DQ 1 TGIF BUS 370 Week 1 DQ 2 Corporate Culture BUS 370 Week 2 DQ 1 Managing Change BUS 370 Week 2 DQ 2 Diagnostic Models BUS 370 Week 3 DQ 1 The Farm Bank BUS 370 Week 3 DQ 2 Communication Processes BUS 370 Week 3 Real World Application of OD Principles BUS 370 Week 4 DQ 1 Goal Setting Model BUS 370 Week 4 DQ 2 Self-Managed Work Teams BUS 370 Week 5 DQ 1 Lafarge BUS...

  16. BUS 600 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 600 Week 1 Assignment Vision Statement BUS 600 Week 1 DQ 1 Importance of Communication BUS 600 Week 1 DQ 2 Ashford Learning Resources BUS 600 Week 2 Assignment Topic Thesis Statement for Research Paper BUS 600 Week 2 DQ 1 Reducing Communication Barriers BUS 600 Week 2 DQ 2 Intercultural Communication Competence BUS 600 Week 3 Assignment Communications Skill Assessment BUS 600 Week 3 DQ 1 Technol...

  17. BUS 600 ASH COURSE TUTORIAL/UOPHELP

    OpenAIRE

    MADHU

    2015-01-01

    For more course tutorials visit   www.uophelp.com   BUS 600 Week 1 Assignment Vision Statement BUS 600 Week 1 DQ 1 Importance of Communication BUS 600 Week 1 DQ 2 Ashford Learning Resources BUS 600 Week 2 Assignment Topic Thesis Statement for Research Paper BUS 600 Week 2 DQ 1 Reducing Communication Barriers BUS 600 Week 2 DQ 2 Intercultural Communication Competence BUS 600 Week 3 Assignment Communications Skill Assessment BUS 600 Week 3 D...

  18. Learning NServiceBus

    CERN Document Server

    Boike, David

    2013-01-01

    This is a practical tutorial containing hands-on examples for creating a messaging and SOA based service bus.This book is for .NET developers who are looking for ways to overcome problems related to buggy third party web service integrations, codebases that have grown into a big ball of mud, and batch jobs failure.

  19. BUS 401 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 401 Week 1 DQ 1 Corporate Income Tax and Real Interest Rates BUS 401 Week 1 DQ 2 Cash Flow and Ratio Analysis BUS 401 Week 1 Assignment Ratio Analysis BUS 401 Week 1 Quiz BUS 401 Week 2 DQ 1 Annuity and Capital Asset Pricing BUS 401 Week 2 DQ 2 Bonds and Common Stock BUS 401 Week 2 Assignment Alternative Investments BUS 401 Week 2 Quiz BUS 401 Week 3 DQ 1 NPV, PI, and IRR BUS 401 Week 3 ...

  20. Behavior and design of reinforced concrete column-type lapped splices subjected to high-intensity cyclic loading

    Science.gov (United States)

    Sivakumar, B.; White, R. N.; Gergely, P.

    1982-10-01

    The behavior and design of lapped splices in reinforced concrete column type specimens under high intensity flexural cyclic loads was studied. Special attention is focused on the transverse steel requirements of specimens with more than two splices in a layer; the use of offsets in spliced bars; the effect of concrete strength on splice strength and behavior; and the strength of epoxy-repaired splices. Procedures are provided for the design of reinforced lapped splices to sustain at least twenty reversing load cycles beyond yield and a maximum rebar strain at the splice of at least 2.5 times the yield strain. The key aspect of the design is the provision of closely spaced uniformly distributed stirrup ties in the splice region. Equations are developed for the spacing of stirrups and the minimum splice length requirement.

  1. BUS 435(ASH) UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+   ASHFORD BUS 435 Week 1 DQ 1 New or Established Venture?   ASHFORD BUS 435 Week 1 DQ 2 Business Opportunity   ASHFORD BUS 435 Week 1 Business Research   ASHFORD BUS 435 Week 2 DQ 1 Franchising   ASHFORD BUS 435 Week 2 DQ 2 Legal Forms   ASHFORD BUS 435 Week 2 Business Concept   ASHFORD BUS 435 Week 3 DQ...

  2. BUS 437 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+   ASHFORD BUS 437 Week 1 Assignment Company Description ASHFORD BUS 437 Week 1 DQ 1 Writing the Business Plan ASHFORD BUS 437 Week 1 DQ 2 Using Outside Help to Write the Plan ASHFORD BUS 437 Week 2 Assignment Marketing Section ASHFORD BUS 437 Week 2 DQ 1 Customer Survey ASHFORD BUS 437 Week 2 DQ 2 Industry Analysis ASHFORD BUS 437 Week 3 Assignment Strategy...

  3. Bus rapid transit

    OpenAIRE

    Wright, Lloyd

    2002-01-01

    Effective public transit is central to development. For the vast majority of developing city residents, public transit is the only practical means to access employment, education, and public services, especially when such services are beyond the viable distance of walking or cycling. Unfortunately, the current state of public transit services in developing cities often does little to serve the actual mobility needs of the population. Bus services are too often unreliable, inconvenient and ...

  4. Circuit breaker and bus

    International Nuclear Information System (INIS)

    Two close-open operations are required each pulse cycle at approximately 3 MA with the breaker in series with the burn supply, both forming a leg in parallel with the E-coil and the homopolar. To conduct the 3-MA current, approximately 30 breaker units are connected in parallel. Each unit is equipped with a counterpulse capacitor bank, and the bus for the 30 units is provided with a common saturable reactor core made of strip steel wound around the bus. Oil breakers of a special design are chosen because of their high arc resistance facilitating the current transfer to the homopolar machine and for obtaining precision timing in opening and closing of the contacts. The precision timing is achieved by a shock wave method with the oil serving as hydraulic medium. Each of the parallel breaker units possesses two sets of contacts, a stationary and a transient one. A 1-mΩ resistor in series with the transient contacts of each breaker unit is to improve the current sharing. The bus consists of room temperature copper, stranded and transposed to help provide current equalization under transient conditions

  5. Complex Alternative Splicing

    OpenAIRE

    Park, Jung Woo; Graveley, Brenton R.

    2007-01-01

    Alternative splicing is a powerful means of controlling gene expression and increasing protein diversity. Most genes express a limited number of mRNA isoforms, but there are several examples of genes that use alternative splicing to generate hundreds, thousands, and even tens of thousands of isoforms. Collectively such genes are considered to undergo complex alternative splicing. The best example is the Drosophila Down syndrome cell adhesion molecule (Dscam) gene, which can generate 38,016 is...

  6. Where splicing joins chromatin

    OpenAIRE

    Hnilicová, Jarmila; Staněk, David

    2011-01-01

    There are numerous data suggesting that two key steps in gene expression—transcription and splicing influence each other closely. For a long time it was known that chromatin modifications regulate transcription, but only recently it was shown that chromatin and histone modifications play a significant role in pre-mRNA splicing. Here we summarize interactions between splicing machinery and chromatin and discuss their potential functional significance. We focus mainly on histone acetylation and...

  7. BUS 402 Ash course tutorial / uophelp

    OpenAIRE

    uophelp-

    2015-01-01

    For more course tutorials visit www.uophelp.com     BUS 402 Week 1 DQ 1 Environmental Changes BUS 402 Week 1 DQ 2 Vision and Mission BUS 402 Week 2 DQ 1 Components of External Environment  BUS 402 Week 2 DQ 2 Components of Internal Analysis BUS 402 Week 2 Assignment Starbucks S.W.O.T. Analysis BUS 402 Week 3 DQ 1 Market Positioning Strategies BUS 402 Week 3 DQ 2 Vertical Integration BUS 402 Week 4 DQ 1 International Markets BUS 40...

  8. BUS 642 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    www.uophelp.com     BUS 642 Week 1 DQ 1 Scientific Thinking (Ash Course) BUS 642 Week 1 DQ 2 Making Research Decisions (Ash Course) BUS 642 Week 1 Exercises (Ash Course) BUS 642 Week 2 DQ 1 Ethics in Business Research (Ash Course) BUS 642 Week 2 DQ 2 Design of Research (Ash Course) BUS 642 Week 2 Exercises (Ash Course) BUS 642 Week 3 DQ 1 Measurement Scales (Ash Course) BUS 642 Week 3 DQ 2 Clarifying the Research Questions (Ash Course) BUS...

  9. BUS 330 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 330 Week 1 DQ 1 Role of the Marketing Function BUS 330 Week 1 DQ 2 Products and Services BUS 330 Week 2 DQ 1 Product Life Cycle BUS 330 Week 2 DQ 2 Product Usage Categories BUS 330 Week 3 DQ 1 Customer Relationship Management BUS 330 Week 3 DQ 2 Demographics and Trends BUS 330 Week 3 Written Assignment (Dramatic Youth Population) BUS 330 Week 4 DQ 1 Functions of Marketing Management BUS 330 ...

  10. BUS 437 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 437 Week 1 Assignment Company Description BUS 437 Week 1 DQ 1 Writing the Business Plan BUS 437 Week 1 DQ 2 Using Outside Help to Write the Plan BUS 437 Week 2 Assignment Marketing Section BUS 437 Week 2 DQ 1 Customer Survey BUS 437 Week 2 DQ 2 Industry Analysis BUS 437 Week 3 Assignment Strategy, Implementation, & Management Sections BUS 437 Week 3 DQ 1 Implementation BUS 437 Week 3 DQ ...

  11. Numerical study of a 50 kA superconducting bus for the NET/ITER model coil test in TOSKA-upgrade

    International Nuclear Information System (INIS)

    Based on the design of the supreconducting bus bar system for the 1.8 K test of the EURATOM LCT coil in the TOSKA Upgrade facility, a superconducting bus bar was designed for the NET/ITER model coil test for an operating current of 50 kA made out of a NbTi cable-in-conduit-conductor imbedded in a copper profile for electrical and mechanical stabilization. For safety reasons, the mass flow through the bus bar is designed as a separate circuit, i.e. the mass flow rates of the coil pancakes, the bus bar, and the current lead are independently adjustable. This results in a bus bar which is safe in case of loss of cooling. But the eddy current losses generated in the stabilizing cooper during the fast discharge of the model coils are by far too high, i.e. the bus bar will quench during high magnetic field changes. Therefore, alternatives are discussed in which the design principles of the bus bar were not changed but it was tried to reduce the eddy current losses in the copper stabilizer. The only possible alternative as a superconducting bus bar consisting of a cable-in-conduit(-CIC)-conductor without any stabilizing copper profile around it. The CIC is imbedded in a jacket made of stainless steel for mechanical stability. The calculations show that this type of bus bar has a high safety margin with respect to AC-losses. In this report, the different designs are described, and the computational results are presented. (orig./GSCH)

  12. 75 FR 5847 - Section 5309 Bus and Bus Facilities Livability Initiative and Urban Circulator Program Grants

    Science.gov (United States)

    2010-02-04

    ...The Federal Transit Administration (FTA) announced on December 8, 2009, the availability of discretionary Section 5309 Bus and Bus Facilities grant and New Starts funds in support of the Department of Transportation's Livability Initiative (``Livability Bus Program''). The Livability Bus Program is funded using $150 million in unallocated Discretionary Bus and Bus Facilities Program funds,......

  13. spliceR

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Porse, Bo Torben; Sandelin, Albin;

    2014-01-01

    RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternative splicing...

  14. 75 FR 23843 - Discretionary Bus and Bus Facilities Program

    Science.gov (United States)

    2010-05-04

    ... initiative. The SGR Bus initiative will make funds available to public transit providers to finance capital... transportation, or private non-profit organizations. Projects eligible for funding under the SGR Bus initiative... Initiative Funds: Solicitation of Project Proposals. SUMMARY: The Federal Transit Administration...

  15. BUS 611 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 611 Week 1 Assignment Article Review (Ash Course) BUS 611 Week 2 Assignment Project Risk (Ash Course) BUS 611 Week 3 Assignment WBS (Ash Course) BUS 611 Week 4 Assignment Integrated Project Management Tools (Ash Course) BUS 611 Week 5 Assignment Monthly Status Reports (Ash Course) BUS 611 Week 6 Final Research Paper (Ash Course)  

  16. BUS 508 STR course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 508 Assignment 1 Entrepreneurial Leadership BUS 508 Assignment 2 Diversification Strategies (Wallmart and Kmart) BUS 508 Week 3 Assignment Production and Operations Management Marathon Oil BUS 508 Assignment 3 Using Teams in Production and Operations Management Boeing Co BUS 508 Assignment 4 Internet Technology, Marketing, and Security (Sony Corp) BUS 508 Assignment 5 Financial Management (Pepsi and Coc...

  17. BUS 330 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

                      For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+     ASHFORD BUS 330 Week 1 DQ 1 Role of the Marketing Function ASHFORD BUS 330 Week 1 DQ 2 Products and Services ASHFORD BUS 330 Week 2 DQ 1 Product Life Cycle ASHFORD BUS 330 Week 2 DQ 2 Product Usage Categories ASHFORD BUS 330 Week 3 DQ 1 Customer Relationship Management ASHFORD BUS...

  18. Learning NServiceBus Sagas

    CERN Document Server

    Helton, Rich

    2015-01-01

    If you are an Enterprise C# developer who wishes to extend your knowledge of NServiceBus and Enterprise Service Bus in C#, this is the book for you. This book is designed to enhance the education of ESBs and their messaging, whether you are a beginner or a seasoned expert in Enterprise C#, Apex, and Visualforce pages.

  19. BUS 620 Ash course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 620 Week 1 DQ 1 What is Marketing (Ash Course) BUS 620 Week 1 DQ 2 Marketing Strategies (Ash Course) BUS 620 Week 1 The Future of the New York Times (Ash Course) BUS 620 Week 2 DQ 1 Buyer Behavior (Ash Course) BUS 620 Week 2 DQ 2 Customer Needs (Ash Course) BUS 620 Week 2 Industry Forecasting (Ash Course) BUS 620 Week 3 DQ 1 Braining Nordstrom (Ash Course) BUS 620 Week 3 DQ 2 Marketing Segmentat...

  20. Cross-kingdom patterns of alternative splicing and splice recognition

    OpenAIRE

    Pearson, Matthew D.; McGuire, Abigail M; Neafsey, Daniel Edward; Galagan, James E.

    2007-01-01

    Background: Variations in transcript splicing can reveal how eukaryotes recognize intronic splice sites. Retained introns (RIs) commonly appear when the intron definition (ID) mechanism of splice site recognition inconsistently identifies intron-exon boundaries, and cassette exons (CEs) are often caused by variable recognition of splice junctions by the exon definition (ED) mechanism. We have performed a comprehensive survey of alternative splicing across 42 eukaryotes to gain ins...

  1. SpliceDisease database: linking RNA splicing and disease

    OpenAIRE

    WANG, Juan; Jie ZHANG; Li, Kaibo; Zhao, Wei; Cui, Qinghua

    2011-01-01

    RNA splicing is an important aspect of gene regulation in many organisms. Splicing of RNA is regulated by complicated mechanisms involving numerous RNA-binding proteins and the intricate network of interactions among them. Mutations in cis-acting splicing elements or its regulatory proteins have been shown to be involved in human diseases. Defects in pre-mRNA splicing process have emerged as a common disease-causing mechanism. Therefore, a database integrating RNA splicing and disease associa...

  2. Splicing regulators: targets and drugs

    OpenAIRE

    Yeo, Gene Wei-Ming

    2005-01-01

    Silencing of splicing regulators by RNA interference, combined with splicing-specific microarrays, has revealed a complex network of distinct alternative splicing events in Drosophila, while a high-throughput screen of more than 6,000 compounds has identified drugs that interfere specifically and directly with one class of splicing regulators in human cells.

  3. Natural gas powered bus

    International Nuclear Information System (INIS)

    This report for the Swiss Federal Office of Energy presents the results of a project carried out by the Swiss Federal Institute of Technology in Zurich to evaluate the performance of a natural-gas-powered bus in comparison with two diesel buses. The report provides details on the vehicles, their routes and the results of interviews made with both passengers and drivers. Details of measurements made on fuel consumption and pollutant emissions are presented in tabular and graphical form, as are those made on noise emissions inside and outside the vehicles. The conclusions of the project are presented including economic aspects of using gas as a motor fuel. Also, the views of passengers, who were more concerned with comfort aspects, and drivers, who were more interested in technical aspects, are quoted

  4. Optical Fiber Fusion Splicing

    CERN Document Server

    Yablon, Andrew D

    2005-01-01

    This book is an up-to-date treatment of optical fiber fusion splicing incorporating all the recent innovations in the field. It provides a toolbox of general strategies and specific techniques that the reader can apply when optimizing fusion splices between novel fibers. It specifically addresses considerations important for fusion splicing of contemporary specialty fibers including dispersion compensating fiber, erbium-doped gain fiber, polarization maintaining fiber, and microstructured fiber. Finally, it discusses the future of optical fiber fusion splicing including silica and non-silica based optical fibers as well as the trend toward increasing automation. Whilst serving as a self-contained reference work, abundant citations from the technical literature will enable readers to readily locate primary sources.

  5. Performance of Grouted Splice Sleeve Connector under Tensile Load

    Directory of Open Access Journals (Sweden)

    A. Alias

    2016-05-01

    Full Text Available The grouted splice sleeve connector system takes advantage of the bond-slip resistance of the grout and the mechanical gripping of reinforcement bars to provide resistance to tensile force. In this system, grout acts as a load-transferring medium and bonding material between the bars and sleeve. This study adopted the end-to-end rebars connection method to investigate the effect of development length and sleeve diameter on the bonding performance of the sleeve connector. The end-to-end method refers to the condition where reinforcement bars are inserted into the sleeve from both ends and meet at the centre before grout is filled. Eight specimens of grouted splice sleeve connector were tested under tensile load to determine their performance. The sleeve connector was designed using 5 mm thick circular hollow section (CHS steel pipe and consisted of one external and two internal sleeves. The tensile test results show that connectors with a smaller external and internal sleeve diameter appear to provide better bonding performance. Three types of failure were observed in this research, which are bar fracture (outside the sleeve, bar pullout, and internal sleeve pullout. With reference to these failure types, the development length of 200 mm is the optimum value due to its bar fracture type, which indicates that the tensile capacity of the connector is higher than the reinforcement bar. It is found that the performance of the grouted splice sleeve connector is influenced by the development length of the reinforcement bar and the diameter of the sleeve.

  6. 高效钢筋挤压连接设备的研究和设计%Research And Design of High Efficiency Squeeze Splicing Machine for the Reinforcing Bars

    Institute of Scientific and Technical Information of China (English)

    钱冠龙

    2001-01-01

    It puts forward the method to increase manufacture efficiency about the squeeze machine for reinforcing bars, and gives the detail on the design idea and technical measures by the large flux and great power. The paper solves the problems such as fever, bad pressurize, parts short life, excessive failure and so on, that happened with flux and part's dimension of 80MPa super high pressure hydraulic system. The machine is being used in many construction projects both at home and abroad.%从提高钢筋挤压连接设备工作效率的几条途径(即提高泵站输出流量、加大挤压钳输出力、实现设备自动控制等)入手,围绕80MPa超高压液压系统提高流量、加大元件尺寸后引出的系统发热、密封效果差、元件寿命低、故障率高等技术难题,阐述了在解决这些难题时大流量、大吨位高效钢筋挤压连接设备其结构、材料、加工技术等方面的设计思想和技术措施。该设备产品已在国内外数百个大中型工程建设项目中得到应用,效果良好。

  7. Hybrid fuel cell bus demonstration: advanced technology moves bus forward

    International Nuclear Information System (INIS)

    The Province of Manitoba has been actively pursuing hydrogen since 2001 as one part of a portfolio of renewable energy alternatives. Six priority hydrogen actions have been underway covering a variety of opportunities, including two recently completed major transit bus and refueling demonstrations. A brief overview of Manitoba's activities on hydrogen will be provided, emphasizing the lessons learned from recent projects such as the hydrogen Hybrid Fuel Cell Bus demonstration, and in particular implications for the research community. (author)

  8. BUS 508 Str course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com     BUS 508 Week 1 Assignment Entrepreneurial Leadership " Five Guys Burgers and Fries: Ingredients for Success BUS 508 Assignment 2:  Competitive Strategies Bus 508 Assignment #3 Forensic Accounting in Practice BUS 508 Assignment 4 Internet Techolology, marketing, and Security    

  9. The Myth of the "Ideal Bus"

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Does as an ideal bus technology exist? Many companies have and will promote a single technology as the ideal bus that will meet all application needs, but in actuality, each bus technology has its own strengths and weaknesses and thus is appropriate for different applications. History has shown the folly of claiming an ideal bus for every application.

  10. BUS 402 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    jonh

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+   ASHFORD BUS 402 Week 1 DQ 1 Environmental Changes ASHFORD BUS 402 Week 1 DQ 2 Vision and Mission ASHFORD BUS 402 Week 2 DQ 1 Components of External Environment ASHFORD BUS 402 Week 2 DQ 2 Components of Internal Analysis ASHFORD BUS 402 Week 2 Assignment Starbucks S.W.O.T. Analysis ASHFORD BUS 402 Week 3 DQ 1 Market Positioning Strategies ASHFORD BUS 402 W...

  11. BUS 670 Ash course tutorial / uophelp

    OpenAIRE

    huphelp

    2015-01-01

    www.uophelp.com     BUS 670 Week 1 DQ 1 Constitutional and Legal Underpinnings of Business Law  BUS 670 Week 1 DQ 2 Ethics and Law  BUS 670 Week 1 Individual Assignment Legal Underpinnings of Business Law  BUS 670 Week 2 DQ 1 Crimes That Harm Business Versus Crimes Committed by Business  BUS 670 Week 2 DQ 2 Product Liability  BUS 670 Week 2 Individual Assignment Negligent Tort  BUS 670 Week 3 DQ 1 Regulation and the G...

  12. Effect of confinement on bond strength of hot-dip galvanized lap splices in concrete structures

    International Nuclear Information System (INIS)

    Galvanizing the reinforcing steel is one of the methods used to protect bars against corrosion. Galvanizing is a hot dip process where the reinforcing bars are immersed in an aqueous pre flux solution of zinc ammonium chloride at a controlled temperature between 840 and 850 degrees F. In 2001, a research program was started at AUB to evaluate experimentally the effect of hot dip galvanizing on the bond capacity of tension lap splices anchored in full-scale beam specimens designed to fail in bond splitting mode. The test results indicated that the use of galvanized bars had a negligible effect on bond strength of reinforcement in normal strength. However, galvanizing caused an average of 20 percent decrease in bond strength of reinforcement in high strength concrete. The primary objective of research reported in this thesis, is the need to find a solution to eliminate the bond reduction of galvanized bars in high strength concrete. It is significant to evaluate the positive effect of the addition of transverse reinforcement in the splice region. The hypothesis to be tested is that such transverse reinforcement will insure uniform bond stress distribution over the entire splice region, thus mobilizing all bar lugs along the splice in the stress transfer mechanism between the bar and the surrounding concrete. Such mechanism might reduce the significant decrease in bond strength in high strength concrete due to galvanizing. To achieve this objective, eighteen full-scale beam specimens were tested in positive bending. Each beam was reinforced with bars spliced in a constant moment region at midspam. The splice length was chosen in such a way that the beams failed in bond splitting of the concrete cover in the splice region. The main variables were type of coating (black or galvanized bars), bar size (20, 25 and 32 mm), and amount of transverse reinforcement in the splice region (0, 2 or 4 stirrups). The test results indicated that confinement did not have a significant

  13. BUS 250 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

                     For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+   ASHFORD BUS 250 Week 1 DQ 1 Business & Society ASHFORD BUS 250 Week 1 DQ 2 Business & Accountability ASHFORD BUS 250 Week 1 Case Study A Brawl in Mickey's Backyard 1 ASHFORD BUS 250 Week 2 Assignment Case Study Alcoa's Core Values in Practice ASHFORD BUS 250 Week 2 DQ 1 Ethical Leadersh...

  14. BUS 599 Str course tutorial / uophelp

    OpenAIRE

    uophelp

    2015-01-01

    For more course tutorials visit www.uophelp.com   BUS 599 Week 1 Assignment Crafting and Executing Strategy Case JetBlue Airways BUS 599 Assignment #2 Competitive Forces and SWOT Analysis # Case Whole Foods Market BUS 599 Week 3 Assignment Foreign Market Entry and Diversification Case Corona Beer BUS 599 Week 4 Assignment HRM Issues Diversification Strategies Case Nucor Corporation BUS 599 Week 5 Assignment Corporate Culture Leadership Actions for Strategy Imp...

  15. BUS 501 Str course tutorial / uophelp

    OpenAIRE

    uophel

    2015-01-01

    For more course tutorials visit www.uophelp.com     BUS 501 Week 3 Assignment 1 The Independent Government Cost Estimate and the Statement of Work (Str Course) BUS 501 Week 4 Assignment 2 Types of Contracts and Performance-Based Acquisition (Str Course) BUS 501 Week 7 Assignment 3 Small Business Program and Source Selection Plans (Str Course) BUS 501 Assignment 4 Technical and Cost or Price Evaluations and Price Reasonableness (Str Course) BUS 501 Wee...

  16. Hydrogen Bus Technology Validation Program

    OpenAIRE

    Burke, Andy; McCaffrey, Zach; Miller, Marshall; Collier, Kirk; Mulligan, Neal

    2005-01-01

    Heavy duty engines are substantial contributors to NOX and PM emissions inventories in urban areas, and stringent emissions standards have been adopted as a consequence. CARB has passed very strict emissions standards for heavy duty engines beginning in 2007. Meeting these standards, especially for NOX emissions, may be difficult for conventional transit bus technologies. The purpose of the Hydrogen Bus Technology Evaluation Program was to develop and evaluate hydrogen enriched natural gas (H...

  17. Bus Stop - Environment Connection: Do Characteristics of the Built Environment Correlate with Bus Stop Crime?

    OpenAIRE

    Robin S Liggett; Loukaitou-Sideris, Anastasia; Iseki, Hiroyuki

    2003-01-01

    Can we understand why some bus stops are safe and others are crime-ridden? Can we predict which features of the bus stop environment are likely to encourage or discourage crime? Can we design safer bus stops? These questions are addressed by exploring the relationship between environmental variables and bus stop crime. An earlier study used crime data, along with environmental indicators, for a sample of 60 bus stops in downtown Los Angeles. Crime rates were higher for bus stops near alleys, ...

  18. Wishbone bus Architecture - A Survey and Comparison

    CERN Document Server

    Sharma, Mohandeep; 10.5121/vlsic.2012.3210

    2012-01-01

    The performance of an on-chip interconnection architecture used for communication between IP cores depends on the efficiency of its bus architecture. Any bus architecture having advantages of faster bus clock speed, extra data transfer cycle, improved bus width and throughput is highly desirable for a low cost, reduced time-to-market and efficient System-on-Chip (SoC). This paper presents a survey of WISHBONE bus architecture and its comparison with three other on-chip bus architectures viz. Advanced Micro controller Bus Architecture (AMBA) by ARM, CoreConnect by IBM and Avalon by Altera. The WISHBONE Bus Architecture by Silicore Corporation appears to be gaining an upper edge over the other three bus architecture types because of its special performance parameters like the use of flexible arbitration scheme and additional data transfer cycle (Read-Modify-Write cycle). Moreover, its IP Cores are available free for use requiring neither any registration nor any agreement or license.

  19. Alternative splicing and muscular dystrophy

    OpenAIRE

    Pistoni, Mariaelena; Ghigna, Claudia; Gabellini, Davide

    2010-01-01

    Alternative splicing of pre-mRNAs is a major contributor to proteomic diversity and to the control of gene expression in higher eukaryotic cells. For this reasons, alternative splicing is tightly regulated in different tissues and developmental stages and its disruption can lead to a wide range of human disorders. The aim of this review is to focus on the relevance of alternative splicing for muscle function and muscle disease. We begin by giving a brief overview of alternative splicing, musc...

  20. BUS 210 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

                      For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 6 Times, Rating: A+   BUS 210 Week 1 DQ1 and DQ2 (UOP Course) BUS 210 Week 1 Checkpoint Business Models and Systems (UOP Course) BUS 210 Week 2 CheckPoint Business Organization (UOP Course) BUS 210 Week 2 Assignment Evolution of Business Presentation (UOP Course) BUS 210 Week 3 DQ 1 and DQ 2 (UOP Course) BUS 210 W...

  1. Where splicing joins chromatin

    Czech Academy of Sciences Publication Activity Database

    Hnilicová, Jarmila; Staněk, David

    2011-01-01

    Roč. 2, č. 3 (2011), s. 182-188. ISSN 1949-1034 R&D Projects: GA ČR GAP305/10/0424; GA AV ČR KAN200520801 Institutional research plan: CEZ:AV0Z50520514 Keywords : chromatin * exon * alternative splicing * transcription * snRNP Subject RIV: EB - Genetics ; Molecular Biology

  2. School bus and children's traffic safety

    Institute of Scientific and Technical Information of China (English)

    PAN Shu-ming; Stephen Hargarten; ZHU Shan-kuan

    2007-01-01

    There is no safer way to transport a child than a school bus. Fatal crashes involving occupants are extremely rare events in the US. In recent years, school bus transportation began to develop in China. We want to bring advanced experience on school bus safety in Western countries such as the US to developing countries.Methods: We searched the papers related to school bus safety from Medline, Chinese Scientific Journals Database and the Web of the National Highway Traffic Safety Administration (NHTSA).Results: There were only 9 papers related to school bus safety, which showed that higher levels of safety standards on school buses, school bus-related transportation and environmental laws and injury prevention were the primary reasons for the desired outcome. Few school bus is related to deaths and injuries in the developed countries.Conclusions: The developing countries should make strict environmental laws and standards on school bus safety to prevent children's injury and death.

  3. Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders

    OpenAIRE

    Pettitt, Jonathan; Müller, Berndt; Stansfield, Ian; Connolly, Bernadette

    2008-01-01

    The trans-splicing of short spliced leader (SL) RNAs onto the 5′ ends of mRNAs occurs in a diverse range of taxa. In nematodes, all species so far characterized utilize a characteristic, conserved spliced leader, SL1, as well as variants that are employed in the resolution of operons. Here we report the identification of spliced leader trans-splicing in the basal nematode Trichinella spiralis, and show that this nematode does not possess a canonical SL1, but rather has at least 15 distinct sp...

  4. HERMES travels by CAN bus

    Science.gov (United States)

    Waller, Lewis G.; Shortridge, Keith; Farrell, Tony J.; Vuong, Minh; Muller, Rolf; Sheinis, Andrew I.

    2014-07-01

    The new HERMES spectrograph represents the first foray by AAO into the use of commercial off-the-shelf industrial field bus technology for instrument control, and we regard the final system, with its relatively simple wiring requirements, as a great success. However, both software and hardware teams had to work together to solve a number of problems integrating the chosen CANopen/CAN bus system into our normal observing systems. A Linux system running in an industrial PC chassis ran the HERMES control software, using a PCI CAN bus interface connected to a number of distributed CANopen/CAN bus I/O devices and servo amplifiers. In the main, the servo amplifiers performed impressively, although some experimentation with homing algorithms was required, and we hit a significant hurdle when we discovered that we needed to disable some of the encoders used during observations; we learned a lot about how servo amplifiers respond when their encoders are turned off, and about how encoders react to losing power. The software was based around a commercial CANopen library from Copley Controls. Early worries about how this heavily multithreaded library would work with our standard data acquisition system led to the development of a very low-level CANopen software simulator to verify the design. This also enabled the software group to develop and test almost all the control software well in advance of the construction of the hardware. In the end, the instrument went from initial installation at the telescope to successful commissioning remarkably smoothly.

  5. Pybus - A Python Software Bus

    International Nuclear Information System (INIS)

    A software bus, just like its hardware equivalent, allows for the discovery, installation, configuration, loading, unloading, and run-time replacement of software components, as well as channeling of inter-component communication. Python, a popular open-source programming language, encourages a modular design on software written in it, but it offers little or no component functionality. However, the language and its interpreter provide sufficient hooks to implement a thin, integral layer of component support. This functionality can be presented to the developer in the form of a module, making it very easy to use. This paper describes a Pythonmodule, PyBus, with which the concept of a ''software bus'' can be realized in Python. It demonstrates, within the context of the ATLAS software framework Athena, how PyBus can be used for the installation and (run-time) configuration of software, not necessarily Python modules, from a Python application in a way that is transparent to the end-user

  6. Pybus -- A Python Software Bus

    Energy Technology Data Exchange (ETDEWEB)

    Lavrijsen, Wim T.L.P.

    2004-10-14

    A software bus, just like its hardware equivalent, allows for the discovery, installation, configuration, loading, unloading, and run-time replacement of software components, as well as channeling of inter-component communication. Python, a popular open-source programming language, encourages a modular design on software written in it, but it offers little or no component functionality. However, the language and its interpreter provide sufficient hooks to implement a thin, integral layer of component support. This functionality can be presented to the developer in the form of a module, making it very easy to use. This paper describes a Pythonmodule, PyBus, with which the concept of a ''software bus'' can be realized in Python. It demonstrates, within the context of the ATLAS software framework Athena, how PyBus can be used for the installation and (run-time) configuration of software, not necessarily Python modules, from a Python application in a way that is transparent to the end-user.

  7. Electrical system architecture having high voltage bus

    Science.gov (United States)

    Hoff, Brian Douglas; Akasam, Sivaprasad

    2011-03-22

    An electrical system architecture is disclosed. The architecture has a power source configured to generate a first power, and a first bus configured to receive the first power from the power source. The architecture also has a converter configured to receive the first power from the first bus and convert the first power to a second power, wherein a voltage of the second power is greater than a voltage of the first power, and a second bus configured to receive the second power from the converter. The architecture further has a power storage device configured to receive the second power from the second bus and deliver the second power to the second bus, a propulsion motor configured to receive the second power from the second bus, and an accessory motor configured to receive the second power from the second bus.

  8. The neurogenetics of alternative splicing

    OpenAIRE

    Vuong, CK; Black, DL; S. Zheng

    2016-01-01

    Alternative precursor-mRNA splicing is a key mechanism for regulating gene expression in mammals and is controlled by specialized RNA-binding proteins. The misregulation of splicing is implicated in multiple neurological disorders. We describe recent mouse genetic studies of alternative splicing that reveal its critical role in both neuronal development and the function of mature neurons. We discuss the challenges in understanding the extensive genetic programmes controlled by proteins that r...

  9. Bar dimensions and bar shapes in estuaries

    Science.gov (United States)

    Leuven, Jasper; Kleinhans, Maarten; Weisscher, Steven; van der Vegt, Maarten

    2016-04-01

    Estuaries cause fascinating patterns of dynamic channels and shoals. Intertidal sandbars are valuable habitats, whilst channels provide access to harbors. We still lack a full explanation and classification scheme for the shapes and dimensions of bar patterns in natural estuaries, in contrast with bars in rivers. Analytical physics-based models suggest that bar length in estuaries increases with flow velocity, tidal excursion length or estuary width, depending on which model. However, these hypotheses were never validated for lack of data and experiments. We present a large dataset and determine the controls on bar shape and dimensions in estuaries, spanning bar lengths from centimeters (experiments) to 10s of kilometers length. First, we visually identified and classified 190 bars, measured their dimensions (width, length, height) and local braiding index. Data on estuarine geometry and tidal characteristics were obtained from governmental databases and literature on case studies. We found that many complex bars can be seen as simple elongated bars partly cut by mutually evasive ebb- and flood-dominated channels. Data analysis shows that bar dimensions scale with estuary dimensions, in particular estuary width. Breaking up the complex bars in simple bars greatly reduced scatter. Analytical bar theory overpredicts bar dimensions by an order of magnitude in case of small estuarine systems. Likewise, braiding index depends on local width-to-depth ratio, as was previously found for river systems. Our results suggest that estuary dimensions determine the order of magnitude of bar dimensions, while tidal characteristics modify this. We will continue to model bars numerically and experimentally. Our dataset on tidal bars enables future studies on the sedimentary architecture of geologically complex tidal deposits and enables studying effects of man-induced perturbations such as dredging and dumping on bar and channel patterns and habitats.

  10. Fusion splicing of silicon optical fibres

    OpenAIRE

    Xiao, L.M.; Healy, N; Gibson, U.; Hawkins, T.; Jones, M.; Ballato, J; A. C. Peacock

    2015-01-01

    The first splicing experiments between silicon optical fibres (SOFs) and conventional fibres are investigated. An optimized fusion splicing approach for a polycrystalline SOF is demonstrated and the material properties after splicing are characterized.

  11. BUS 600 ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    ben

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 5 Times, Rating: A+   BUS 600 Week 1 Assignment Vision Statement BUS 600 Week 1 DQ 1 Importance of Communication BUS 600 Week 1 DQ 2 Ashford Learning Resources BUS 600 Week 2 Assignment Topic Thesis Statement for Research Paper BUS 600 Week 2 DQ 1 Reducing Communication Barriers BUS 600 Week 2 DQ 2 Intercultural Communication Competence BUS 600 Week 3 Assignment Communications...

  12. BUS 620 ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    atwater

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A   ASHFORD BUS 620 Week 1 DQ 1 What is Marketing   ASHFORD BUS 620 Week 1 DQ 2 Marketing Strategies   ASHFORD BUS 620 Week 1 Assignment Tesco Case   ASHFORD BUS 620 Week 2 DQ 1 Buyer Behavior   ASHFORD BUS 620 Week 2 DQ 2 Customer Needs   ASHFORD BUS 620 Week 2 Industry Forecasting   ASHFORD BUS 620 Week 3...

  13. BUS 660 ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    john

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+   BUS 660 Week 1 Assignment Developing Leaders at UPS  BUS 660 Week 2 Assignment Leadership Style  BUS 660 Week 3 Assignment Leadership Traits  BUS 660 Week 4 Assignment Leadership Skills  BUS 660 Week 5 Assignment Big Changes for a Small Hospital  BUS 660 Week 6 Assignment Research Paper BUS 660 Week 1 DQ 1Leadership Defined BU...

  14. BUS 644 (New) ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    ashford

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating:  A+     ASHFORD BUS 644 Week 1 DQ 1 Ethical Issues and Operations (New) ASHFORD BUS 644 Week 1 DQ 2 Business Processes (New) ASHFORD BUS 644 Week 1 Midas Case Study (New) ASHFORD BUS 644 Week 2 DQ 1 Productivity (New) ASHFORD BUS 644 Week 2 DQ 2 Product Design and Quality (New) ASHFORD BUS 644 Week 2 Memorial Hospital Case Study (New) ASHFORD BUS...

  15. New TPG bus route 28

    CERN Multimedia

    2003-01-01

    Geneva's Public Transport services (TPG) have recently inaugurated a new bus line 28, connecting the La Tour Hospital in Meyrin to the international organisations in Geneva, via the airport. All signs associated with this route will be yellow in colour. Timetables and route details can be found at http://www.tpg.ch. Relations with the Host States Service http://www.cern.ch/relations/ Tel. 72848

  16. Inverse splicing of a group II intron.

    OpenAIRE

    Jarrell, K A

    1993-01-01

    I describe the self-splicing of an RNA that consists of exon sequences flanked by group II intron sequences. I find that this RNA undergoes accurate splicing in vitro, yielding an excised exon circle. This splicing reaction involves the joining of the 5' splice site at the end of an exon to the 3' splice site at the beginning of the same exon; thus, I term it inverse splicing. Inverse splicing provides a potential mechanism for exon scrambling, for exon deletion in alternative splicing pathwa...

  17. Bus Routes, Marta Bus Routes located in Transportation database, Published in unknown, City of Roswell, GA.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset as of unknown. It is described as 'Marta Bus Routes located in Transportation database'. Data by this publisher are often provided in State...

  18. YUTONG BUS: Grateful To The Financial Crisis

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Shenyin&Wanguo Securities issued an announcement on Jan 9 rating Yutong Bus(600066)as"buying",on the basis that the bus maker's gross profits went better in the last quarter of 2008 and to be further improved in the first quarter of 2009,with an increased market share. The report drew attention inside the in- dustry when the entire bus segment was suffering a tough time.

  19. Wishbone Bus Architecture - A Survey and Comparison

    Directory of Open Access Journals (Sweden)

    Mohandeep Sharma

    2012-05-01

    Full Text Available The performance of an on-chip interconnection architecture used for communication between IP cores depends on the efficiency of its bus architecture. Any bus architecture having advantages of faster bus clock speed, extra data transfer cycle, improved bus width and throughput is highly desirable for a low cost, reduced time-to-market and efficient System-on-Chip (SoC. This paper presents a survey of WISHBONE bus architecture and its comparison with three other on-chip bus architectures viz. Advanced Microcontroller Bus Architecture (AMBA by ARM, CoreConnect by IBM and Avalon by Altera. The WISHBONE Bus Architecture by Silicore Corporation appears to be gaining an upper edge over the other three bus architecture types because of its special performance parameters like the use of flexible arbitration scheme and additional data transfer cycle (Read-Modify-Write cycle. Moreover, its IP Cores are available free for use requiring neither any registration nor any agreement or license.

  20. Bus transport in Greater Manchester

    DEFF Research Database (Denmark)

    Sørensen, Claus Hedegaard; Gudmundsson, Henrik

    . Important elements have been: Division of previously horizontal integrated organisations, corporatisation, privatisation, public-private partnerships, tendering and management by goals and results. The main purpose of the NPM reforms has been to increase the efficiency, effectiveness and accountability of...... first component regards the organisation of public bus transport at the local level. This involves the deregulation and privatisation reforms introduced by the Conservative Government from 1986 onwards, as well as the ‘partnerships’ and other new instruments introduced by the Labour Government in the...

  1. Intronic Alus influence alternative splicing.

    Directory of Open Access Journals (Sweden)

    Galit Lev-Maor

    Full Text Available Examination of the human transcriptome reveals higher levels of RNA editing than in any other organism tested to date. This is indicative of extensive double-stranded RNA (dsRNA formation within the human transcriptome. Most of the editing sites are located in the primate-specific retrotransposed element called Alu. A large fraction of Alus are found in intronic sequences, implying extensive Alu-Alu dsRNA formation in mRNA precursors. Yet, the effect of these intronic Alus on splicing of the flanking exons is largely unknown. Here, we show that more Alus flank alternatively spliced exons than constitutively spliced ones; this is especially notable for those exons that have changed their mode of splicing from constitutive to alternative during human evolution. This implies that Alu insertions may change the mode of splicing of the flanking exons. Indeed, we demonstrate experimentally that two Alu elements that were inserted into an intron in opposite orientation undergo base-pairing, as evident by RNA editing, and affect the splicing patterns of a downstream exon, shifting it from constitutive to alternative. Our results indicate the importance of intronic Alus in influencing the splicing of flanking exons, further emphasizing the role of Alus in shaping of the human transcriptome.

  2. MapSplice: Accurate mapping of RNA-seq reads for splice junction discovery

    OpenAIRE

    Kai WANG; Singh, Darshan; Zeng, Zheng; Coleman, Stephen J.; Huang, Yan; Savich, Gleb L.; He, Xiaping; Mieczkowski, Piotr; Grimm, Sara A; Perou, Charles M; MacLeod, James N; Chiang, Derek Y.; Prins, Jan F.; Liu, Jinze

    2010-01-01

    The accurate mapping of reads that span splice junctions is a critical component of all analytic techniques that work with RNA-seq data. We introduce a second generation splice detection algorithm, MapSplice, whose focus is high sensitivity and specificity in the detection of splices as well as CPU and memory efficiency. MapSplice can be applied to both short (

  3. A new bus lane on urban expressway with no-bay bus stop

    Science.gov (United States)

    Tian, Zhao; Jia, Limin

    2016-01-01

    The sharp increase in residents and vehicles causes heavy traffic pressure in many cities. To ease traffic congestion, it has been the common sense that we should develop public transit system. The priority of the bus appears particularly necessary with the rapid development of the public transport system. The bus lane is an important embodiment of the bus priority. Focusing on the problem of the unreasonable dedicated bus lane (DBL) under the lower ratio of buses, this paper proposed a new bus lane with limited physical length. And this bus lane can reduce the lane-changing conflict caused by the buses and cars running on roads without bus lanes. Based on the cellular automata (CA) traffic flow model and the lane-changing behavior of the vehicle including the optional lane-changing and the mandatory lane-changing, a three-lane traffic model with an isolated no-bay bus stop is proposed. The ordinary three-lane traffic without a bus lane and the cases of traffic with a DBL or the proposed bus lane are simulated, and the comparisons in the form of the fundamental diagrams are made among them. It is shown that the no-bay bus stop can act as a bottleneck on the traffic flow because of the mandatory lane-changing behavior. Under a certain ratio of the bus number to the total vehicles number, (1) the traffic with the proposed bus lane has less lane-changing conflict and can provide higher traffic capacity than the ordinary traffic without a bus lane, (2) compared with the DBL, the proposed bus lane is advantageous in easing congestion on the ordinary lanes when the traffic flow is high and can avoid unreasonable allocation of the road resources.

  4. BUS 670 ASH Course Tutorial/TutorialRank

    OpenAIRE

    kuran

    2015-01-01

    ASHFORD BUS 670 Entire Course For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+     ASHFORD BUS 670 Week 1 DQ 1 Constitutional and Legal Underpinnings of Business Law ASHFORD BUS 670 Week 1 DQ 2 Ethics and Law ASHFORD BUS 670 Week 1 Individual Assignment Legal Underpinnings of Business Law ASHFORD BUS 670 Week 2 DQ 1 Crimes That Harm Business Versus Crimes Committed by Business ASHFORD BUS 670 Week 2 ...

  5. Conserved RNA secondary structures promote alternative splicing

    OpenAIRE

    Shepard, PJ; Hertel, KJ

    2008-01-01

    Pre-mRNA splicing is carried out by the spliceosome, which identifies exons and removes intervening introns. Alternative splicing in higher eukaryotes results in the generation of multiple protein isoforms from gene transcripts. The extensive alternative splicing observed implies a flexibility of the spliceosome to identify exons within a given pre-mRNA. To reach this flexibility, splice-site selection in higher eukaryotes has evolved to depend on multiple parameters such as splice-site stren...

  6. Engineering splicing factors with designed specificities

    OpenAIRE

    Wang, Yang; Cheong, Cheom-Gil; Hall, Traci M Tanaka; Wang, Zefeng

    2009-01-01

    Alternative splicing is generally regulated by trans-acting factors that specifically bind pre-mRNA to activate or inhibit the splicing reaction. This regulation is critical for normal gene expression, and dysregulation of splicing is closely associated with human diseases. Here we engineer artificial splicing factors by combining sequence-specific RNA-binding domains of human Pumilio1 with functional domains that regulate splicing. We applied these factors to modulate different types of alte...

  7. Mastering NServiceBus and persistence

    CERN Document Server

    Helton, Rich

    2014-01-01

    This book is intended for developers, designers, and architects alike who wish to build C# NServiceBus enterprise architectures and learn how ESB persists data and messages to help them attain their goals. No prior knowledge of persistence in NServiceBus is required.

  8. Integrating many co-splicing networks to reconstruct splicing regulatory modules

    OpenAIRE

    Dai Chao; Li Wenyuan; Liu Juan; Zhou Xianghong

    2012-01-01

    Abstract Background Alternative splicing is a ubiquitous gene regulatory mechanism that dramatically increases the complexity of the proteome. However, the mechanism for regulating alternative splicing is poorly understood, and study of coordinated splicing regulation has been limited to individual cases. To study genome-wide splicing regulation, we integrate many human RNA-seq datasets to identify splicing module, which we define as a set of cassette exons co-regulated by the same splicing f...

  9. Alternative Splicing Regulation During C. elegans Development: Splicing Factors as Regulated Targets

    OpenAIRE

    Sergio Barberan-Soler; Zahler, Alan M.

    2008-01-01

    Alternative splicing generates protein diversity and allows for post-transcriptional gene regulation. Estimates suggest that 10% of the genes in Caenorhabditis elegans undergo alternative splicing. We constructed a splicing-sensitive microarray to detect alternative splicing for 352 cassette exons and tested for changes in alternative splicing of these genes during development. We found that the microarray data predicted that 62/352 (approximately 18%) of the alternative splicing events studi...

  10. Barred Owl [ds8

    Data.gov (United States)

    California Department of Resources — These data define the current range of Barred and hybrid Barred/Spotted Owls in California. The current range includes the coastal mountains of northern California...

  11. Spliced-leader trans-splicing in freshwater planarians.

    Science.gov (United States)

    Zayas, Ricardo M; Bold, Tyler D; Newmark, Phillip A

    2005-10-01

    trans-Splicing, in which a spliced-leader (SL) RNA is appended to the most 5' exon of independently transcribed pre-mRNAs, has been described in a wide range of eukaryotes, from protozoans to chordates. Here we describe trans-splicing in the freshwater planarian Schmidtea mediterranea, a free-living member of the phylum Platyhelminthes. Analysis of an expressed sequence tag (EST) collection from this organism showed that over 300 transcripts shared one of two approximately 35-base sequences (Smed SL-1 and SL-2) at their 5' ends. Examination of genomic sequences encoding representatives of these transcripts revealed that these shared sequences were transcribed elsewhere in the genome. RNA blot analysis, 5' and 3' rapid amplification of cDNA ends, as well as genomic sequence data showed that 42-nt SL sequences were derived from small RNAs of approximately 110 nt. Similar sequences were also found at the 5' ends of ESTs from the planarian Dugesia japonica. trans-Splicing has already been described in numerous representatives of the phylum Platyhelminthes (trematodes, cestodes, and polyclads); its presence in two representatives of the triclads supports the hypothesis that this mode of RNA processing is ancestral within this group. The upcoming complete genome sequence of S. mediterranea, combined with this animal's experimental accessibility and susceptibility to RNAi, provide another model organism in which to study the function of the still-enigmatic trans-splicing. PMID:15972844

  12. Drivetrain system of an ultracapacitor electric bus

    Institute of Scientific and Technical Information of China (English)

    Kong Zhigu; Zhu Chunbo; Yang Shiyan; Cheng Shukang

    2007-01-01

    This paper proposed a design of the drivetrain system of an electric bus with ultracapacitor (UC) as the only on-board power source. The system includes three main parts, namely, UC bank, motor and the converter, vehicle management unit (VMU). Analyses results in detail on the functional design and experiments on work bench of each part were also presented, which validated the reliability of the system. Furthermore, driving results in field of the bus verified the feasibility of the design of the drivetrain system. The bus has very good dynamic performances and shows a promising applications prospect in the short and medium route buses system.

  13. MapSplice: accurate mapping of RNA-seq reads for splice junction discovery.

    Science.gov (United States)

    Wang, Kai; Singh, Darshan; Zeng, Zheng; Coleman, Stephen J; Huang, Yan; Savich, Gleb L; He, Xiaping; Mieczkowski, Piotr; Grimm, Sara A; Perou, Charles M; MacLeod, James N; Chiang, Derek Y; Prins, Jan F; Liu, Jinze

    2010-10-01

    The accurate mapping of reads that span splice junctions is a critical component of all analytic techniques that work with RNA-seq data. We introduce a second generation splice detection algorithm, MapSplice, whose focus is high sensitivity and specificity in the detection of splices as well as CPU and memory efficiency. MapSplice can be applied to both short (<75 bp) and long reads (≥ 75 bp). MapSplice is not dependent on splice site features or intron length, consequently it can detect novel canonical as well as non-canonical splices. MapSplice leverages the quality and diversity of read alignments of a given splice to increase accuracy. We demonstrate that MapSplice achieves higher sensitivity and specificity than TopHat and SpliceMap on a set of simulated RNA-seq data. Experimental studies also support the accuracy of the algorithm. Splice junctions derived from eight breast cancer RNA-seq datasets recapitulated the extensiveness of alternative splicing on a global level as well as the differences between molecular subtypes of breast cancer. These combined results indicate that MapSplice is a highly accurate algorithm for the alignment of RNA-seq reads to splice junctions. Software download URL: http://www.netlab.uky.edu/p/bioinfo/MapSplice. PMID:20802226

  14. Estimated errors in vertical bar Vcd vertical bar / vertical bar Vcs vertical bar from semileptonic D decays

    International Nuclear Information System (INIS)

    We estimate statistical and systematic errors in the extraction of the CKM ratio vertical bar Vcd vertical bar / vertical bar Vcs vertical bar from exclusive D-meson semileptonic decays using lattice QCD and anticipated new experimental results

  15. Incremental bus service design: combining limited-stop and local bus services

    OpenAIRE

    Chiraphadhanakul, Virot; Barnhart, Cynthia

    2013-01-01

    Long in-vehicle travel times resulting from frequent stops make bus service an unattractive choice for many commuters. Limited-stop bus services however have the advantage of shorter in-vehicle times experienced by passengers. In this work, we seek to modify a given bus service by optimally reassigning some number of bus trips, as opposed to providing additional trips, to operate a limited-stop service. We propose an optimization model to determine a limited-stop service route to be operated ...

  16. DESIGN AND EXPERIMENT OF ELECTRIC BUS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Performance and reliability experiments on BJD-6100EV electric buses, jointly developed by Beijing Institute of Technology and Beijing Public Transport Company, are introduced. Output power model of battery pack is established and maximum output power is analyzed. A permanent magnetic direct current (PMDC) motor with enhanced windings is developed for the bus. Torque-speed characteristics of the motor are modeled and performance of electric bus is analyzed. Computational method of the range of electric bus is proposed and discussed. Experiments show that electric bus can realize design requirements. Computational methods are verified with the help of field test. It is expected that design and computation method will provide helpful reference to development of electric vehicles.

  17. BUS 599 STRAYER Course Tutorial/TutorialRank

    OpenAIRE

    alfonos

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+ STRAYER BUS 599 Week 1 Assignment Crafting and Executing Strategy Case JetBlue Airways STRAYER BUS 599 Week 2 Assignment Competitive Forces and SWOT Analysis Case Whole Foods Market STRAYER BUS 599 Week 3 Assignment Foreign Market Entry and Diversification Case Corona Beer STRAYER BUS 599 Week 4 Assignment HRM Issues Diversification Strategies Case Nucor Corporation STRAYER BUS 5...

  18. BUS 670 ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    bertoz

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+     ASHFORD BUS 670 Week 1 DQ 1 Constitutional and Legal Underpinnings of Business Law ASHFORD BUS 670 Week 1 DQ 2 Ethics and Law ASHFORD BUS 670 Week 1 Individual Assignment Legal Underpinnings of Business Law ASHFORD BUS 670 Week 2 DQ 1 Crimes That Harm Business Versus Crimes Committed by Business ASHFORD BUS 670 Week 2 DQ 2 Product Liability ASHFORD...

  19. TMS communications software. Volume 2: Bus interface unit

    Science.gov (United States)

    Gregor, P. J.

    1979-01-01

    A data bus communication system to support the space shuttle's Trend Monitoring System (TMS) and to provide a basis for evaluation of the bus concept is described. Installation of the system included developing both hardware and software interfaces between the bus and the specific TMS computers and terminals. The software written for the microprocessor-based bus interface units is described. The software implements both the general bus communications protocol and also the specific interface protocols for the TMS computers and terminals.

  20. Bus Network Modeling Using Ant Algorithms

    OpenAIRE

    Sepideh Eshragh; Shahriar Afandizadeh Zargari; Ardeshir Faghri; Earl Rusty Lee

    2010-01-01

    Bus transit network modeling is a complex and combinatorial problem. The main purpose of this paper is to apply a contemporary method for designing a bus transit network with the objective of achieving optimum results. The method is called Ant Algorithms, a Meta Heuristic method, which has been applied to optimization problems in transportation with noticeable success. The description of the algorithm, as well as the main methodology and computations, is presented in this paper. Furthermore, ...

  1. Hydrogen hybrid internal combustion engine transit bus

    International Nuclear Information System (INIS)

    The Province of Manitoba has been actively pursuing hydrogen since 2001 as one part of a portfolio of renewable energy alternatives. Six priority hydrogen actions have been underway covering a variety of opportunities, including two recently completed major transit bus and refueling demonstrations. A brief overview of Manitoba's activities on hydrogen will be provided, emphasizing the lessons learned from recent projects such as the hydrogen Hybrid Fuel Cell Bus demonstration, and in particular implications for the research community. (author)

  2. Systems evaluation of thermal bus concepts

    Science.gov (United States)

    Stalmach, D. D.

    1982-01-01

    Thermal bus concepts, to provide a centralized thermal utility for large, multihundred kilowatt space platforms, were studied and the results are summarized. Concepts were generated, defined, and screened for inclusion in system level thermal bus trades. Parametric trade studies were conducted in order to define the operational envelope, performance, and physical characteristics of each. Two concepts were selected as offering the most promise for thermal bus development. All of four concepts involved two phase flow in order to meet the required isothermal nature of the thermal bus. Two of the concepts employ a mechanical means to circulate the working fluid, a liquid pump in one case and a vapor compressor in another. Another concept utilizes direct osmosis as the driving force of the thermal bus. The fourth concept was a high capacity monogroove heat pipe. After preliminary sizing and screening, three of these concepts were selected to carry into the trade studies. The monogroove heat pipe concept was deemed unsuitable for further consideration because of its heat transport limitations. One additional concept utilizing capillary forces to drive the working fluid was added. Parametric system level trade studies were performed. Sizing and weight calculations were performed for thermal bus sizes ranging from 5 to 350 kW and operating temperatures in the range of 4 to 120 C. System level considerations such as heat rejection and electrical power penalties and interface temperature losses were included in the weight calculations.

  3. EASI—enrichment of alternatively spliced isoforms

    OpenAIRE

    Julian P Venables; Burn, John

    2006-01-01

    Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To investigate the true extent of alternative splicing we have developed a simple method of enriching alternatively spliced isoforms (EASI) from PCRs using beads charged with Thermus aquaticus single-stranded DNA-binding protein (T.Aq ssb). This ...

  4. Mechano-Regulation of Alternative Splicing

    OpenAIRE

    Liu, Huan; Tang, Liling

    2013-01-01

    Alternative splicing contributes to the complexity of proteome by producing multiple mRNAs from a single gene. Affymetrix exon arrays and experiments in vivo or in vitro demonstrated that alternative splicing was regulated by mechanical stress. Expression of mechano-growth factor (MGF) which is the splicing isoform of insulin-like growth factor 1(IGF-1) and vascular endothelial growth factor (VEGF) splicing variants such as VEGF121, VEGF165, VEGF206, VEGF189, VEGF165 and VEGF145 are regulated...

  5. ASD: a bioinformatics resource on alternative splicing

    OpenAIRE

    Stamm, Stefan; Riethoven, Jean-Jack; Le Texier, Vincent; Gopalakrishnan, Chellappa; Kumanduri, Vasudev; Tang, Yesheng; Barbosa-Morais, Nuno L.; Thanaraj, Thangavel Alphonse

    2005-01-01

    Alternative splicing is an important regulatory mechanism of mammalian gene expression. The alternative splicing database (ASD) consortium is systematically collecting and annotating data on alternative splicing. We present the continuation and upgrade of the ASD [T. A. Thanaraj, S. Stamm, F. Clark, J. J. Riethoven, V. Le Texier, J. Muilu (2004) Nucleic Acids Res. 32, D64–D69] that consists of computationally and manually generated data. Its largest parts are AltSplice, a value-added database...

  6. Targeting RNA Splicing for Disease Therapy

    OpenAIRE

    Havens, Mallory A.; Duelli, Dominik M.; Hastings, Michelle L.

    2013-01-01

    Splicing of pre-messenger RNA into mature messenger RNA is an essential step for expression of most genes in higher eukaryotes. Defects in this process typically affect cellular function and can have pathological consequences. Many human genetic diseases are caused by mutations that cause splicing defects. Furthermore, a number of diseases are associated with splicing defects that are not attributed to overt mutations. Targeting splicing directly to correct disease-associated aberrant splicin...

  7. Evolution of alternative splicing after gene duplication

    OpenAIRE

    Su, Zhixi; Wang, Jianmin; Yu, Jun; Huang, Xiaoqiu; Gu, Xun

    2006-01-01

    Alternative splicing and gene duplication are two major sources of proteomic function diversity. Here, we study the evolutionary trend of alternative splicing after gene duplication by analyzing the alternative splicing differences between duplicate genes. We observed that duplicate genes have fewer alternative splice (AS) forms than single-copy genes, and that a negative correlation exists between the mean number of AS forms and the gene family size. Interestingly, we found that the loss of ...

  8. SAW: A Method to Identify Splicing Events from RNA-Seq Data Based on Splicing Fingerprints

    OpenAIRE

    Kang Ning; Damian Fermin

    2010-01-01

    Splicing event identification is one of the most important issues in the comprehensive analysis of transcription profile. Recent development of next-generation sequencing technology has generated an extensive profile of alternative splicing. However, while many of these splicing events are between exons that are relatively close on genome sequences, reads generated by RNA-Seq are not limited to alternative splicing between close exons but occur in virtually all splicing events. In this work, ...

  9. Alternative splicing and trans-splicing events revealed by analysis of the Bombyx mori transcriptome

    OpenAIRE

    Shao, Wei; Zhao, Qiong-Yi; Wang, Xiu-Ye; Xu, Xin-Yan; Tang, Qing; Li, Muwang; Li, Xuan; Xu, Yong-Zhen

    2012-01-01

    Alternative splicing and trans-splicing events have not been systematically studied in the silkworm Bombyx mori. Here, the silkworm transcriptome was analyzed by RNA-seq. The authors identified 320 novel genes, modified 1140 gene models, and found thousands of alternative splicing and 58 trans-splicing events. Studies of three SR proteins show that both their alternative splicing patterns and mRNA products are conserved from insect to human, and one isoform of Srsf6 with a retained intron is ...

  10. 77 FR 6178 - FY 2012 Discretionary Funding Opportunities: Bus and Bus Facilities Programs (State of Good...

    Science.gov (United States)

    2012-02-07

    ... fosters sustainability and the development of urban and rural land use. Through Bus Livability Initiative... Programs (State of Good Repair and Bus Livability Initiatives) and Clean Fuels Grant Program, Augmented...), DOT. ACTION: Notice of Funding Availability for FTA State of Good Repair, Livability,...

  11. Dynamic Bus Travel Time Prediction Models on Road with Multiple Bus Routes

    Science.gov (United States)

    Bai, Cong; Peng, Zhong-Ren; Lu, Qing-Chang; Sun, Jian

    2015-01-01

    Accurate and real-time travel time information for buses can help passengers better plan their trips and minimize waiting times. A dynamic travel time prediction model for buses addressing the cases on road with multiple bus routes is proposed in this paper, based on support vector machines (SVMs) and Kalman filtering-based algorithm. In the proposed model, the well-trained SVM model predicts the baseline bus travel times from the historical bus trip data; the Kalman filtering-based dynamic algorithm can adjust bus travel times with the latest bus operation information and the estimated baseline travel times. The performance of the proposed dynamic model is validated with the real-world data on road with multiple bus routes in Shenzhen, China. The results show that the proposed dynamic model is feasible and applicable for bus travel time prediction and has the best prediction performance among all the five models proposed in the study in terms of prediction accuracy on road with multiple bus routes. PMID:26294903

  12. COMMUNICATION: Alternative splicing and genomic stability

    Science.gov (United States)

    Cahill, Kevin

    2004-06-01

    Alternative splicing allows an organism to make different proteins in different cells at different times, all from the same gene. In a cell that uses alternative splicing, the total length of all the exons is much shorter than in a cell that encodes the same set of proteins without alternative splicing. This economical use of exons makes genes more stable during reproduction and development because a genome with a shorter exon length is more resistant to harmful mutations. Genomic stability may be the reason why higher vertebrates splice alternatively. For a broad class of alternatively spliced genes, a formula is given for the increase in their stability.

  13. GC content around splice sites affects splicing through pre-mRNA secondary structures

    Directory of Open Access Journals (Sweden)

    Chen Liang

    2011-01-01

    Full Text Available Abstract Background Alternative splicing increases protein diversity by generating multiple transcript isoforms from a single gene through different combinations of exons or through different selections of splice sites. It has been reported that RNA secondary structures are involved in alternative splicing. Here we perform a genomic study of RNA secondary structures around splice sites in humans (Homo sapiens, mice (Mus musculus, fruit flies (Drosophila melanogaster, and nematodes (Caenorhabditis elegans to further investigate this phenomenon. Results We observe that GC content around splice sites is closely associated with the splice site usage in multiple species. RNA secondary structure is the possible explanation, because the structural stability difference among alternative splice sites, constitutive splice sites, and skipped splice sites can be explained by the GC content difference. Alternative splice sites tend to be GC-enriched and exhibit more stable RNA secondary structures in all of the considered species. In humans and mice, splice sites of first exons and long exons tend to be GC-enriched and hence form more stable structures, indicating the special role of RNA secondary structures in promoter proximal splicing events and the splicing of long exons. In addition, GC-enriched exon-intron junctions tend to be overrepresented in tissue-specific alternative splice sites, indicating the functional consequence of the GC effect. Compared with regions far from splice sites and decoy splice sites, real splice sites are GC-enriched. We also found that the GC-content effect is much stronger than the nucleotide-order effect to form stable secondary structures. Conclusion All of these results indicate that GC content is related to splice site usage and it may mediate the splicing process through RNA secondary structures.

  14. Titin Diversity—Alternative Splicing Gone Wild

    Directory of Open Access Journals (Sweden)

    Wei Guo

    2010-01-01

    Full Text Available Titin is an extremely large protein found in highest concentrations in heart and skeletal muscle. The single mammalian gene is expressed in multiple isoforms as a result of alternative splicing. Although titin isoform expression is controlled developmentally and in a tissue specific manner, the vast number of potential splicing pathways far exceeds those described in any other alternatively spliced gene. Over 1 million human splice pathways for a single individual can be potentially derived from the PEVK region alone. A new splicing pattern for the human cardiac N2BA isoform type has been found in which the PEVK region includes only the N2B type exons. The alterations in splicing and titin isoform expression in human heart disease provide impetus for future detailed study of the splicing mechanisms for this giant protein.

  15. BUS 630 ASHFORD Course Tutorial/TutorialRank

    OpenAIRE

    avery

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 4 Times, Rating: A+ ASHFORD BUS 630 Week 1 Assignment Case 2B Mendel Paper Company (Old) ASHFORD BUS 630 Week 1 DQ 1 Ethics in Cost Control (Old) ASHFORD BUS 630 Week 1 DQ 2 Fixed and Variable costs (Old) ASHFORD BUS 630 Week 2 DQ 1 Product costs (Old) ASHFORD BUS 630 Week 2 DQ 2 Job Order Costing vs. Proess Costing (Old) ASHFORD BUS 630 Week 2 Assignment Case 3A (Auerbach Enterprises) (Old) ...

  16. Designing Bus Structure for Digital Controller of Nuclear Power Plant

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Gil; Park, Gang Min; Lee, Myeong Kyun; Yoo, Kwan Woo; Yun, Dong Hwa [Research Institute, PONUTech, Daejeon (Korea, Republic of)

    2014-08-15

    In this paper, the bus structure design for the digital controller of Nuclear Power Plant (NPP) is proposed. The most important portion is the bus in the digital controller. A bus is responsible for transmission data among the various Input/Output (I/O) board, Processor board and Communication board. An existing bus is a parallel bus structure such as the VERSA Module Euro (VME) bus and an extended Static Random Access Memory (SRAM) Controller. Central Processing Unit (CPU) processing speed and communication speed is faster than before, but the parallel bus has a speed limit. Because it is the physically lines shared and the weakness about reflected wave. That is, use of the parallel bus has been the cause of the performance degradation of system. To solve the parallel bus problem, the bus has been developed by serial. The configuration of transceiver logic was simplified by not share the bus that all boards and processor boards was connected to the point-to-point. Serial bus was configured using physically Low Voltage Differential Signaling (LVDS). The speed limit of bus was broken by the LVDS what is consisted of a strong against the reflected-wave of signal, noise and etc. Each channel of bus has a different speed mode and is able to set a required transmission way. Different transmission ways of each channel are able to transmit data to match the required response time of system. The LVDS speed has about basically 1Gbps but the bus was composed with less than 50Mbps considering the environmental characteristics of nuclear. It was shown that the configuration of the serial bus was the performance and the reliability more improving than parallel bus.

  17. Designing Bus Structure for Digital Controller of Nuclear Power Plant

    International Nuclear Information System (INIS)

    In this paper, the bus structure design for the digital controller of Nuclear Power Plant (NPP) is proposed. The most important portion is the bus in the digital controller. A bus is responsible for transmission data among the various Input/Output (I/O) board, Processor board and Communication board. An existing bus is a parallel bus structure such as the VERSA Module Euro (VME) bus and an extended Static Random Access Memory (SRAM) Controller. Central Processing Unit (CPU) processing speed and communication speed is faster than before, but the parallel bus has a speed limit. Because it is the physically lines shared and the weakness about reflected wave. That is, use of the parallel bus has been the cause of the performance degradation of system. To solve the parallel bus problem, the bus has been developed by serial. The configuration of transceiver logic was simplified by not share the bus that all boards and processor boards was connected to the point-to-point. Serial bus was configured using physically Low Voltage Differential Signaling (LVDS). The speed limit of bus was broken by the LVDS what is consisted of a strong against the reflected-wave of signal, noise and etc. Each channel of bus has a different speed mode and is able to set a required transmission way. Different transmission ways of each channel are able to transmit data to match the required response time of system. The LVDS speed has about basically 1Gbps but the bus was composed with less than 50Mbps considering the environmental characteristics of nuclear. It was shown that the configuration of the serial bus was the performance and the reliability more improving than parallel bus

  18. Statistical Analysis of Bus Networks in India

    CERN Document Server

    Chatterjee, Atanu; Ramadurai, Gitakrishnan

    2015-01-01

    Through the past decade the field of network science has established itself as a common ground for the cross-fertilization of exciting inter-disciplinary studies which has motivated researchers to model almost every physical system as an interacting network consisting of nodes and links. Although public transport networks such as airline and railway networks have been extensively studied, the status of bus networks still remains in obscurity. In developing countries like India, where bus networks play an important role in day-to-day commutation, it is of significant interest to analyze its topological structure and answer some of the basic questions on its evolution, growth, robustness and resiliency. In this paper, we model the bus networks of major Indian cities as graphs in \\textit{L}-space, and evaluate their various statistical properties using concepts from network science. Our analysis reveals a wide spectrum of network topology with the common underlying feature of small-world property. We observe tha...

  19. Spliced leader RNA trans-splicing discovered in copepods

    Science.gov (United States)

    Yang, Feifei; Xu, Donghui; Zhuang, Yunyun; Yi, Xiaoyan; Huang, Yousong; Chen, Hongju; Lin, Senjie; Campbell, David A.; Sturm, Nancy R.; Liu, Guangxing; Zhang, Huan

    2015-12-01

    Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3‧-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods.

  20. Notes on the fast bus system

    International Nuclear Information System (INIS)

    In any system there will be modules that gather and process data from the experiment's detectors and perhaps from other sources such as the accelerator control system or monitoring system. In order to have a general system these modules must be able to communicate with each other and perhaps with a host computer or a group of controlling computers. A very general communications system is the bus system, used in computers, in which all devices are connected in parallel to a group of lines, typically 50 to 100. By means of a protocol of communication on some of these lines, a device that wants to control the bus makes its wishes known. If the device is the highest priority one currently asking for the bus, it gains bus mastership and then signals another device that it wants to communicate with by means of a set of address lines. This system works very well in a computer that includes a processor, memory, and peripheral devices; its disadvantage is that only one device can be bus master at a time and only one communication can take place at a time. The proposed solution to this problem is to segment the bus so that each segment can operate independently without interfering with another one. This allows a large amount of parallel operation. It is, of course, necessary for one segment to communicate with other ones at some point in the process, and this is achieved by a device called the segment interconnect (SI). Much of the character of the system will be determined by the details of the SI. One possibility is a device that passes information only when the address falls within a band of values (or perhaps several bands); details of its specification are being worked on now

  1. Risk factors affecting fatal bus accident severity: Their impact on different types of bus drivers.

    Science.gov (United States)

    Feng, Shumin; Li, Zhenning; Ci, Yusheng; Zhang, Guohui

    2016-01-01

    While the bus is generally considered to be a relatively safe means of transportation, the property losses and casualties caused by bus accidents, especially fatal ones, are far from negligible. The reasons for a driver to incur fatalities are different in each case, and it is essential to discover the underlying risk factors of bus fatality severity for different types of drivers in order to improve bus safety. The current study investigates the underlying risk factors of fatal bus accident severity to different types of drivers in the U.S. by estimating an ordered logistic model. Data for the analysis are retrieved from the Buses Involved in Fatal Accidents (BIFA) database from the USA for the years 2006-2010. Accidents are divided into three levels by counting their equivalent fatalities, and the drivers are classified into three clusters by the K-means cluster analysis. The analysis shows that some risk factors have the same impact on different types of drivers, they are: (a) season; (b) day of week; (c) time period; (d) number of vehicles involved; (e) land use; (f) manner of collision; (g) speed limit; (h) snow or ice surface condition; (i) school bus; (j) bus type and seating capacity; (k) driver's age; (l) driver's gender; (m) risky behaviors; and (n) restraint system. Results also show that some risk factors only have impact on the "young and elder drivers with history of traffic violations", they are: (a) section type; (b) number of lanes per direction; (c) roadway profile; (d) wet road surface; and (e) cyclist-bus accident. Notably, history of traffic violations has different impact on different types of bus drivers. PMID:26513334

  2. Secure Bus-Ticketing System using NFC

    Directory of Open Access Journals (Sweden)

    Monalisa Lopes

    2015-04-01

    Full Text Available For making the day to day life more convenient for the passengers travelling in bus some technologies can be used like Near Field Communication (NFC, Radio Frequency Identification (RFID. The proposed system is based on ticketing and identification of passengers in public transport. The system suggest a user friendly automated ticketing system which will automatically deduct the passengers fare according to the distance travelled as well as detect the passengers detail information. The system also helps to calculate the total revenue of a single bus in a day. Another important aspect is reusability, which helps use ticket multiple times as it is rechargeable.

  3. NOC AND BUS ARCHITECTURE: A COMPARISON

    Directory of Open Access Journals (Sweden)

    RAJEEV KAMAL

    2012-04-01

    Full Text Available Network-on-chip designs promise to offer considerable advantages over the traditional bus-based architecture. As continuing scaling of Moore’s law enables ever greater transistor densities, design complexity, power limitations and application convergence networks have started to replace busses in much smaller systems and the enhancement of NoC. This paper summarizes the advantages of the NoC and the limitations of traditional bus based architecture. In this paper we discuss a detailed comparison of area, power, scalability andperformance of traditional busses in comparison with NoC.

  4. Bus Network Modeling Using Ant Algorithms

    Directory of Open Access Journals (Sweden)

    Sepideh Eshragh

    2010-02-01

    Full Text Available Bus transit network modeling is a complex and combinatorial problem. The main purpose of this paper is to apply a contemporary method for designing a bus transit network with the objective of achieving optimum results. The method is called Ant Algorithms, a Meta Heuristic method, which has been applied to optimization problems in transportation with noticeable success. The description of the algorithm, as well as the main methodology and computations, is presented in this paper. Furthermore, a case study using Ant Algorithms applied to the city of Ghazvin, one of the most important suburbs of Tehran, Iran, is presented.

  5. Work fatigue in urban bus drivers

    OpenAIRE

    Teresa Makowiec-Dąbrowska; Jadwiga Siedlecka; Elżbieta Gadzicka; Agata Szyjkowska; Marta Dania; Piotr Viebig; Marcin Kosobudzki; Alicja Bortkiewicz

    2015-01-01

    Background: Bus drivers are a special group of professional drivers who are at a very high risk of fatigue. The aim of the study was to examine whether the driver’s subjective assessment of fatigue allows for the determination of its level and identification of its causes. Material and Methods: The study group comprised 45 randomly selected bus drivers (mean age – 43.7±7.9 years, period of employment as drivers – 14.7±8.6 years). Examinations were performed in all subjects four times – before...

  6. Implementing a Large Data Bus VLIW Microprocessor

    OpenAIRE

    Weng F. Lee; Ali Y.M. Shakaff

    2008-01-01

    Microprocessors have grown tremendously in its computing and data crunching capability since the early days of the invention of a microprocessor. Today, most microprocessors in the market are at 32 bits, while the latest microprocessors from IBM, Intel and AMD are at 64 bits. To further grow the computational capability of a microprocessor, there are two possible paths. One method is to increase the data bus size of the microprocessor to 128/256/512 bits. The larger the data bus size, the mor...

  7. BHLS, Bus, tram: tesi, antitesi, sintesi.

    OpenAIRE

    López Lambas, Maria Eugenia; Valdés Serrano, Cristina

    2013-01-01

    Il sistema di trasporto detto Bus Rapid Transit (BRT) è stato lanciato a Curitiba, in Brasile, nel 1974 per offrire un trasporto in bus efficiente ed efficace nella città in rapida espansione. Questa sperienza, insieme a quella di Ottawa (1983) e Quito (1994) ha dimostrato di essere una soluzione molto efficiente per il trasporto di massa. Per tutta l’Europa si sono iniziate a sviluppare esperienze simili, introducendo però un concetto diverso per quanto riguarda la qualità di servizio. In...

  8. Harmonizing the reinforcement development and splice length for next gen nuclear construction

    International Nuclear Information System (INIS)

    In nuclear power plant construction, both Safety-Related and Non-Safety-Related structures are present. Safety-Related RC structures will generally include Concrete Containment and other Category I (CAT I) structures. The reinforcement development/splice length for reinforcement in containment structures is provided according to ASME SECTION III Div 2 (ACI 359-07) Code. The development and splice length requirements for other Safety-Related CAT I RC Structures are given in ACI 349-06, Code requirements for Nuclear Safety-Related Concrete Structures. The development and splice length requirements for Non-Safety structures are given in ACI 318-08. Unfortunately, the requirements in the three Codes (ACI 359, ACI 349 and ACI 318) are not the same and can vary significantly. This results, for example, in the same bar size requiring different development/splice lengths for different structures which is extra work for the fabricator and also increases the potential for confusion/mix up at a particular job site. This evaluates and discusses the differences in development and splice length requirements in the above-mentioned Codes for CAT I and Non-Safety-Related structures and explores opportunities for harmonization of requirements among the three Codes, where possible and appropriate. The development and splice length requirement for higher grade reinforcement (Gr 75 and Gr 80), which are expected to be allowed in the next editions of the US nuclear codes will also be discussed. Based on this study, some recommendations are made to streamline the development/splice requirements to help improve construction of New Gen nuclear construction

  9. SpliceProt: a protein sequence repository of predicted human splice variants.

    Science.gov (United States)

    Tavares, Raphael; de Miranda Scherer, Nicole; Pauletti, Bianca Alves; Araújo, Elói; Folador, Edson Luiz; Espindola, Gabriel; Ferreira, Carlos Gil; Paes Leme, Adriana Franco; de Oliveira, Paulo Sergio Lopes; Passetti, Fabio

    2014-02-01

    The mechanism of alternative splicing in the transcriptome may increase the proteome diversity in eukaryotes. In proteomics, several studies aim to use protein sequence repositories to annotate MS experiments or to detect differentially expressed proteins. However, the available protein sequence repositories are not designed to fully detect protein isoforms derived from mRNA splice variants. To foster knowledge for the field, here we introduce SpliceProt, a new protein sequence repository of transcriptome experimental data used to investigate for putative splice variants in human proteomes. Current version of SpliceProt contains 159 719 non-redundant putative polypeptide sequences. The assessment of the potential of SpliceProt in detecting new protein isoforms resulting from alternative splicing was performed by using publicly available proteomics data. We detected 173 peptides hypothetically derived from splice variants, which 54 of them are not present in UniprotKB/TrEMBL sequence repository. In comparison to other protein sequence repositories, SpliceProt contains a greater number of unique peptides and is able to detect more splice variants. Therefore, SpliceProt provides a solution for the annotation of proteomics experiments regarding splice isofoms. The repository files containing the translated sequences of the predicted splice variants and a visualization tool are freely available at http://lbbc.inca.gov.br/spliceprot. PMID:24273012

  10. Design of E-City Bus Tracking System

    Directory of Open Access Journals (Sweden)

    Shekhar Shinde

    2014-04-01

    Full Text Available E-city bus tracking system will serve as a viable notification system that will effectively assist passengers in making the decision of whether to wait for the bus or not. This system is a standalone system designed to display the real-time location(s of the buses with the use of GSM module technology. The system will consist of an IR transmitter module installed on the buses, receiver boards installed on the bus stops and GSM receiver connected with PC monitoring the whole arrangement. It will also have passenger information system software installed at the bus stops and which will provide user the relevant information regarding all the bus numbers going for his source to destination along with the route details. It will also transmit its bus numbers and route names, with the help of LED transmitter, continuously as soon as the bus comes within the range of the receiver at the bus stop.

  11. Dissecting brick into bars

    OpenAIRE

    Feshchenko, Ivan; Radchenko, Danylo; Radzivilovsky, Lev; Tantsiura, Maksym

    2008-01-01

    An $N$-dimensional parallelepiped will be called a bar if and only if there are no more than $k$ different numbers among the lengths of its sides (the definition of bar depends on $k$). We prove that a parallelepiped can be dissected into finite number of bars iff the lengths of sides of the parallelepiped span a linear space of dimension no more than $k$ over $\\QQ$. This extends and generalizes a well-known theorem of Max Dehn about partition of rectangles into squares. Several other results...

  12. Framework for Bus Rapid Transit Development and Deployment Planning

    OpenAIRE

    Miller, Mark A.; Yin, Yafeng; Balvanyos, Tunde; Ceder, Avishai

    2004-01-01

    This report presents the results of its investigation into deployment planning for bus rapid transit systems. In this study, we conducted a macro-scale examination of bus rapid transit systems from technical, operational, institutional, and planning perspectives. We then developed the theoretical foundation for a deployment planning framework for bus rapid transit systems that specifically takes into account the unique features of bus rapid transit that differ from other transit alternatives....

  13. Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.

    OpenAIRE

    Hicks, Martin J; Chin-Rang Yang; Matthew V Kotlajich; Hertel, Klemens J.

    2006-01-01

    RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II ( Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol II-synthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because the local concentration of the sp...

  14. Control of Alternative Splicing by Signal-dependent Degradation of Splicing-regulatory Proteins*S⃞

    OpenAIRE

    Katzenberger, Rebeccah J.; Marengo, Matthew S.; Wassarman, David A.

    2009-01-01

    Alternative pre-mRNA splicing is a major gene expression regulatory mechanism in metazoan organisms. Proteins that bind pre-mRNA elements and control assembly of splicing complexes regulate utilization of pre-mRNA alternative splice sites. To understand how signaling pathways impact this mechanism, an RNA interference screen in Drosophila S2 cells was used to identify proteins that regulate TAF1 (TBP-associated factor 1) alternative splicing in response to activation o...

  15. Competition between Pre-mRNAs for the splicing machinery drives global regulation of splicing

    OpenAIRE

    Munding, EM; Shiue, L; Katzman, S.; Donohue, J; Ares, M

    2013-01-01

    During meiosis in yeast, global splicing efficiency increases and then decreases. Here we provide evidence that splicing improves due to reduced competition for the splicing machinery. The timing of this regulation corresponds to repression and reactivation of ribosomal protein genes (RPGs) during meiosis. In vegetative cells, RPG repression by rapamycin treatment also increases splicing efficiency. Downregulation of the RPG-dedicated transcription factor gene IFH1 genetically suppresses two ...

  16. Regulation of Splicing Factors by Alternative Splicing and NMD Is Conserved between Kingdoms Yet Evolutionarily Flexible

    OpenAIRE

    Liana F Lareau; Brenner, Steven E.

    2015-01-01

    Ultraconserved elements, unusually long regions of perfect sequence identity, are found in genes encoding numerous RNA-binding proteins including arginine-serine rich (SR) splicing factors. Expression of these genes is regulated via alternative splicing of the ultraconserved regions to yield mRNAs that are degraded by nonsense-mediated mRNA decay (NMD), a process termed unproductive splicing (Lareau et al. 2007; Ni et al. 2007). As all human SR genes are affected by alternative splicing and N...

  17. Evolutionary conservation of alternative splicing in chicken

    OpenAIRE

    Katyal, S.; Gao, Z.; Liu, R.-Z.; R Godbout

    2007-01-01

    Alternative splicing represents a source of great diversity for regulating protein expression and function. It has been estimated that one-third to two-thirds of mammalian genes are alternatively spliced. With the sequencing of the chicken genome and analysis of transcripts expressed in chicken tissues, we are now in a position to address evolutionary conservation of alternative splicing events in chicken and mammals. Here, we compare chicken and mammalian transcript sequences of 41 alternati...

  18. SPA: a probabilistic algorithm for spliced alignment.

    OpenAIRE

    Erik van Nimwegen; Nicodeme Paul; Robert Sheridan; Mihaela Zavolan

    2006-01-01

    Recent large-scale cDNA sequencing efforts show that elaborate patterns of splice variation are responsible for much of the proteome diversity in higher eukaryotes. To obtain an accurate account of the repertoire of splice variants, and to gain insight into the mechanisms of alternative splicing, it is essential that cDNAs are very accurately mapped to their respective genomes. Currently available algorithms for cDNA-to-genome alignment do not reach the necessary level of accuracy because the...

  19. Repeater For A Digital-Communication Bus

    Science.gov (United States)

    Torres-Guzman, Esteban; Olson, Stephen; Heaps, Tim

    1993-01-01

    Digital repeater circuit designed to extend range of communication on MIL-STD-1553 bus beyond original maximum allowable length of 300 ft. Circuit provides two-way communication, one way at time, and conforms to specifications of MIL-STD-1553. Crosstalk and instability eliminated.

  20. Implementing a Large Data Bus VLIW Microprocessor

    Directory of Open Access Journals (Sweden)

    Weng F. Lee

    2008-01-01

    Full Text Available Microprocessors have grown tremendously in its computing and data crunching capability since the early days of the invention of a microprocessor. Today, most microprocessors in the market are at 32 bits, while the latest microprocessors from IBM, Intel and AMD are at 64 bits. To further grow the computational capability of a microprocessor, there are two possible paths. One method is to increase the data bus size of the microprocessor to 128/256/512 bits. The larger the data bus size, the more data can be crunched at any one time. The second method is to implement multiple microprocessor core in a single microprocessor unit. For example, Intel's Pentium 4 Dual Core and AMD's Athlon Dual Core both have two microprocessor core within a single microprocessor unit. Latest from Intel and AMD are quad core microprocessors with four microprocessor core within a single microprocessor unit. Both methods have its advantages and disadvantages. Both methods yields different design issues and have different engineering limitations. This research looks into the possibility of implementing a large data bus size VLIW microprocessor core of 256 bits on the data bus. VLIW is chosen as opposed to CISC and RISC due to its ease of scalability.

  1. School Bus Accidents: Reducing Incidents and Injuries

    Science.gov (United States)

    Mahoney, Daniel

    2009-01-01

    The number of children injured in nonfatal school bus accidents annually is more than double the number previously estimated. In Ohio alone, approximately 20,800 children younger than 18 were occupants of school buses that were involved in crashes in 2003 and 2004 (McGeehan 2007). Among those children, most had minor or no injuries. However, there…

  2. Sports Season, Sports Bars

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ For foreigners in Beijing, the sports bar is a special place, a place to gather for watching matches and a place to feel the familiarity of home, while for some sports enthusiasts it serves as their second home.

  3. An appraisal of QR code use to deliver bus arrival time information at bus stops in Southampton

    OpenAIRE

    Gammer, Nick

    2011-01-01

    The provision of at-stop bus arrival time information has numerous potential benefits to bus users. With real-time passenger information well established in the UK the challenge is relaying accurate bus arrival times to passengers in a cost effective manner. Display screens are costly to install and maintain, rendering placement at all bus stops economically undesirable. QR codes represent an inexpensive alternative to delivering this information, usually at zero marginal cost to users. Q...

  4. Virginia School Bus Driver Training: Does Training Program Adequacy Affect school Bus Accident Rates?

    OpenAIRE

    Crews, James O

    1997-01-01

    Analysis of data provided by the Virginia Departments of Motor Vehicles, Transportation, and Education all show school buses as having a significantly higher number of accidents per hundred million miles of travel than other forms of transportation. Such statistics are alarming and formal study of school bus accident factors has been limited. The purposes of this study were to (a) establish the adequacy of the school bus driver training programs of Local Education Agencies(LEAs...

  5. Bus 54 -- Where are you? A school bus intelligent information system

    Energy Technology Data Exchange (ETDEWEB)

    Truett, L.F.; Moore, S.; Tonn, B. [Oak Ridge National Lab., TN (United States); Conley, T. [GTE Wireless, Nashville, TN (United States)

    1998-07-01

    Although major accidents involving school buses are rare (only about 0.3% of all fatal crashes since 1986 are classified as school-bus-related), even minor accidents and breakdowns cause a great deal of parental anxiety. The objective of this research is to design an efficient, cost-effective, accurate, and secure system that will track individual school buses and communicate appropriate information to the school system`s central administration unit, to the school transportation administrator, and to parents of children on the bus. The greatest benefit of the proposed information system is that parents and officials can always know the location and condition (these conditions would vary depending on the needs of a particular school system) of the school buses. In case of an accident or mechanical problem, when emergency crews are needed, they can be dispatched almost immediately with a good understanding of the problem and the exact location of the bus. In addition to being able to track the bus while the child is on board, parents will be able to determine the location of their child`s bus prior to its arrival in the morning in order to prevent the child from needing to wait outside in inclement weather. The information available to parents can also be expanded to include maps of limited routes (e.g., snow routes). Basically, the Bus 54 concept consists of a bus component and a central data processing unit. Each bus will be outfitted with a global positioning satellite (GPS) device, a wireless communication device, and wireless data communication service. The central data processing unit will receive and process information from the buses and provide information access to parents and officials via an Internet link.

  6. The bar instability revisited

    OpenAIRE

    Chiodi, Filippo; Andreotti, Bruno; Claudin, Philippe

    2012-01-01

    The river bar instability is revisited, using a hydrodynamical model based on Reynolds averaged Navier-Stokes equations. The results are contrasted with the standard analysis based on shallow water Saint-Venant equations. We first show that the stability of both transverse modes (ripples) and of small wavelength inclined modes (bars) predicted by the Saint-Venant approach are artefacts of this hydrodynamical approximation. When using a more reliable hydrodynamical model, the dispersion relati...

  7. Recursive splicing in long vertebrate genes.

    Science.gov (United States)

    Sibley, Christopher R; Emmett, Warren; Blazquez, Lorea; Faro, Ana; Haberman, Nejc; Briese, Michael; Trabzuni, Daniah; Ryten, Mina; Weale, Michael E; Hardy, John; Modic, Miha; Curk, Tomaž; Wilson, Stephen W; Plagnol, Vincent; Ule, Jernej

    2015-05-21

    It is generally believed that splicing removes introns as single units from precursor messenger RNA transcripts. However, some long Drosophila melanogaster introns contain a cryptic site, known as a recursive splice site (RS-site), that enables a multi-step process of intron removal termed recursive splicing. The extent to which recursive splicing occurs in other species and its mechanistic basis have not been examined. Here we identify highly conserved RS-sites in genes expressed in the mammalian brain that encode proteins functioning in neuronal development. Moreover, the RS-sites are found in some of the longest introns across vertebrates. We find that vertebrate recursive splicing requires initial definition of an 'RS-exon' that follows the RS-site. The RS-exon is then excluded from the dominant mRNA isoform owing to competition with a reconstituted 5' splice site formed at the RS-site after the first splicing step. Conversely, the RS-exon is included when preceded by cryptic promoters or exons that fail to reconstitute an efficient 5' splice site. Most RS-exons contain a premature stop codon such that their inclusion can decrease mRNA stability. Thus, by establishing a binary splicing switch, RS-sites demarcate different mRNA isoforms emerging from long genes by coupling cryptic elements with inclusion of RS-exons. PMID:25970246

  8. $\\{Q\\bar{q}\\}\\{\\bar{Q}^{(')}q\\}$ molecular states

    OpenAIRE

    Zhang, Jian-Rong; Huang, Ming-Qiu(College of Science, National University of Defense Technology, 410073, Hunan, China)

    2009-01-01

    Masses for $\\{Q\\bar{q}\\}\\{\\bar{Q}^{(')}q\\}$ molecular states are systematically studied in QCD sum rules. The interpolating currents representing the related molecular states are proposed. Technically, contributions of the operators up to dimension six are included in operator product expansion (OPE). Mass spectra for molecular states with $\\{Q\\bar{q}\\}\\{\\bar{Q}^{(')}q\\}$ configurations are obtained.

  9. Advanced eGovernment Information Service Bus (eGov-Bus

    Directory of Open Access Journals (Sweden)

    2007-01-01

    Full Text Available The eGov-Bus project provides citizens and businesses with improved access to virtual public services, which are based on existing national eGovernment Web services and which support cross-border life events. Requirements and specific rules of these life events are considered, and personalization of user preferences is supported. eGov-Bus is based on adaptable process management technologies, allowing for virtual services which are dynamically combined from existing national eGovernment services. In this way, a comprehensive workflow process is set up, allowing for service-level agreements, an audit trail and explanation of the process to the end user. The eGov-Bus process engine operates on top of a virtual repository, providing a high-level semantic view of information retrieved from heterogeneous information sources, such as eGovernment Web services. Further, eGov-Bus relies on a security framework to ensure all high-level security requirements are met. The eGov-Bus architecture is business oriented, it focuses on Service Oriented Architecture (SOA concepts, asynchronously combining Web services and providing a Service Bus.

  10. Alternative mRNA Splicing: Control by Combination

    OpenAIRE

    Mabon, Stephen A; Tom Misteli

    2005-01-01

    Alternative splicing in mammalian cells has been suggested to be largely controlled by combinatorial binding of basal splicing factors to pre-mRNA templates. This model predicts that distinct sets of pre-mRNA splicing factors are associated with alternatively spliced transcripts. However, no experimental evidence for differential recruitment of splicing factors to transcripts with distinct splicing fates is available. Here we have used quantitative single-cell imaging to test this key predict...

  11. SplicingTypesAnno: annotating and quantifying alternative splicing events for RNA-Seq data.

    Science.gov (United States)

    Sun, Xiaoyong; Zuo, Fenghua; Ru, Yuanbin; Guo, Jiqiang; Yan, Xiaoyan; Sablok, Gaurav

    2015-04-01

    Alternative splicing plays a key role in the regulation of the central dogma. Four major types of alternative splicing have been classified as intron retention, exon skipping, alternative 5 splice sites or alternative donor sites, and alternative 3 splice sites or alternative acceptor sites. A few algorithms have been developed to detect splice junctions from RNA-Seq reads. However, there are few tools targeting at the major alternative splicing types at the exon/intron level. This type of analysis may reveal subtle, yet important events of alternative splicing, and thus help gain deeper understanding of the mechanism of alternative splicing. This paper describes a user-friendly R package, extracting, annotating and analyzing alternative splicing types for sequence alignment files from RNA-Seq. SplicingTypesAnno can: (1) provide annotation for major alternative splicing at exon/intron level. By comparing the annotation from GTF/GFF file, it identifies the novel alternative splicing sites; (2) offer a convenient two-level analysis: genome-scale annotation for users with high performance computing environment, and gene-scale annotation for users with personal computers; (3) generate a user-friendly web report and additional BED files for IGV visualization. SplicingTypesAnno is a user-friendly R package for extracting, annotating and analyzing alternative splicing types at exon/intron level for sequence alignment files from RNA-Seq. It is publically available at https://sourceforge.net/projects/splicingtypes/files/ or http://genome.sdau.edu.cn/research/software/SplicingTypesAnno.html. PMID:25720307

  12. Energy efficiency of different bus subsystems in Belgrade public transport

    Directory of Open Access Journals (Sweden)

    Mišanović Slobodan M.

    2015-01-01

    Full Text Available Research in this paper comprised experimental determination of energy efficiency of different bus subsystems (diesel bus, trolleybus and fully electric bus on chosen public transport route in Belgrade. Experimental measuring of energy efficiency of each bus type has been done based on the analysis of parameters of vehicle driving cycles between stops. Results of this analysis were basis for development of theoretical simulation model of energy efficiency. The model was latter compared with the results of simulation done by "Solaris bus & Coach" company for the chosen electric bus route. Based on demonstrated simulation, characteristics of electric bus batteries were defined, the method and dynamic of their recharge was suggested, as well as choice for other aggregates for drive system and technical characteristics for the electric buses were suggested.

  13. Large-scale comparative analysis of splicing signals and their corresponding splicing factors in eukaryotes

    OpenAIRE

    Schwartz, Schraga; Silva, João(CFTP, Departamento de Física, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais 1, 1049, Lisboa, Portugal); Burstein, David; Pupko, Tal; Eyras, Eduardo; Ast, Gil

    2008-01-01

    Introns are among the hallmarks of eukaryotic genes. Splicing of introns is directed by three main splicing signals: the 5′ splice site (5′ss), the branch site (BS), and the polypyrimdine tract/3′splice site (PPT-3′ss). To study the evolution of these splicing signals, we have conducted a systematic comparative analysis of these signals in over 1.2 million introns from 22 eukaryotes. Our analyses suggest that all these signals have dramatically evolved: The PPT is weak among most fungi, inter...

  14. MICROSAT: A New, Small Satellite Bus Concept

    OpenAIRE

    Duncan, Courtney

    1989-01-01

    The Radio Amateur Satellite Corporation of North America (AMSAT-NA) is pioneering a new, small satellite bus concept known as "Microsat." The product is roughly a nine inch cube, exclusive of antennas, with five sub-assemblies. Four are standard: radio transmitter(s), radio receiver(s), power system, and onboard computer. The fifth module is reserved for payload or special application hardware. Design goals dictate that the cost of the satellites and the cost and complexity of ground terminal...

  15. BUS 475 UOP Course Tutorial/ Tutorialrank

    OpenAIRE

    john

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 6 Times, Rating: A+   BUS 475 Capstone Final Examination Part 1   1.    In the managerial function of __________, managers encourage and motivate workers to work toward organizational goals.   2.    eBay.com is a vast auction site that is similar to a competitive market in some ways but differs from it in others. Which of the following describes...

  16. Exposure Assessment of Diesel Bus Emissions

    Directory of Open Access Journals (Sweden)

    Werner Hofmann

    2006-12-01

    Full Text Available The goal of this study was to measure ultrafine particle concentrations with diameters less than 1 μm emitted by diesel buses and to assess resulting human exposure levels. The study was conducted at the Woolloongabba Busway station in Brisbane, Australia in the winter months of 2002 during which temperature inversions frequently occurred. Most buses that utilize the station are fuelled by diesel, the exhaust of which contains a significant quantity of particle matter. Passengers waiting at the station are exposed to these particles emitted from the buses. During the course of this study, passenger census was conducted, based on video surveillance, yielding person-by-person waiting time data. Furthermore, a bus census revealed accurate information about the total number of diesel versus Compressed Natural Gas (CNG powered buses. Background (outside of the bus station and platform measurements of ultrafine particulate number size distributions were made to determine ambient aerosol concentrations. Particle number exposure concentration ranges from 10 and 40 to 60% of bus related exhaust fumes. This changes dramatically when considering the particle mass exposure concentration, where most passengers are exposed to about 50 to 80% of exhaust fumes. The obtained data can be very significant for comparison with similar work of this type because it is shown in previous studies that exhaust emissions causes cancer in laboratory animals. It was assumed that significant differences between platform and background distributions were due to bus emissions which, combined with passenger waiting times, yielded an estimate of passenger exposure to ultrafine particles from diesel buses. From an exposure point of view, the Busway station analyzed resembles a street canyon. Although the detected exhaust particle concentration at the outbound platform is found to be in the picogram range, exposure increases with the time passengers spend on the platform

  17. Brazilian hybrid electric fuel cell bus

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, P.E.V.; Carreira, E.S. [Coppe-Federal Univ. of Rio de Janeiro (Brazil). Hydrogen Lab.

    2010-07-01

    The first prototype of a hybrid electric fuel cell bus developed with Brazilian technology is unveiled. It is a 12 m urban-type, low-floor, air-conditioned bus that possesses three doors, air suspension, 29 seats and reversible wheelchair site. The bus body was built based on a double-deck type monoblock vehicle that is able to sustain important load on its roof. This allowed positioning of the type 3 hydrogen tanks and the low weight traction batteries on the roof of the vehicles without dynamic stabilization problems. A novel hybrid energy configuration was designed in such a way that the low-power (77 kWe) fuel cell works on steady-state operation mode, not responding directly to the traction motor load demand. The rate of kinetic energy regeneration upon breaking was optimized by the use of an electric hybrid system with predominance of batteries and also by utilizing supercapacitors. The electric-electronic devices and the security control softwares for the auxiliary and traction systems were developed in-house. The innovative hybrid-electric traction system configuration led to the possibility to decrease the fuel cell power, with positive impact on weight and system volume reduction, as well as to significantly decrease the hydrogen consumption. (orig.)

  18. Spliced

    DEFF Research Database (Denmark)

    Addison, Courtney Page

    2016-01-01

    Human gene therapy (HGT) aims to cure disease by inserting or editing the DNA of patients with genetic conditions. Since foundational genetic techniques came into use in the 1970s, the field has developed to the point that now three therapies have market approval, and over 1800 clinical trials have......-work stretches out from science to enlist diverse publics, social formations and the natural world in the pursuit of legitimacy....

  19. Alternative splicing regulation during C. elegans development: splicing factors as regulated targets.

    Directory of Open Access Journals (Sweden)

    Sergio Barberan-Soler

    2008-02-01

    Full Text Available Alternative splicing generates protein diversity and allows for post-transcriptional gene regulation. Estimates suggest that 10% of the genes in Caenorhabditis elegans undergo alternative splicing. We constructed a splicing-sensitive microarray to detect alternative splicing for 352 cassette exons and tested for changes in alternative splicing of these genes during development. We found that the microarray data predicted that 62/352 (approximately 18% of the alternative splicing events studied show a strong change in the relative levels of the spliced isoforms (>4-fold during development. Confirmation of the microarray data by RT-PCR was obtained for 70% of randomly selected genes tested. Among the genes with the most developmentally regulated alternatively splicing was the hnRNP F/H splicing factor homolog, W02D3.11 - now named hrpf-1. For the cassette exon of hrpf-1, the inclusion isoform comprises 65% of hrpf-1 steady state messages in embryos but only 0.1% in the first larval stage. This dramatic change in the alternative splicing of an alternative splicing factor suggests a complex cascade of splicing regulation during development. We analyzed splicing in embryos from a strain with a mutation in the splicing factor sym-2, another hnRNP F/H homolog. We found that approximately half of the genes with large alternative splicing changes between the embryo and L1 stages are regulated by sym-2 in embryos. An analysis of the role of nonsense-mediated decay in regulating steady-state alternative mRNA isoforms was performed. We found that 8% of the 352 events studied have alternative isoforms whose relative steady-state levels in embryos change more than 4-fold in a nonsense-mediated decay mutant, including hrpf-1. Strikingly, 53% of these alternative splicing events that are affected by NMD in our experiment are not obvious substrates for NMD based on the presence of premature termination codons. This suggests that the targeting of splicing factors

  20. Position dependence of the rous sarcoma virus negative regulator of splicing element reflects proximity to a 5' splice site

    International Nuclear Information System (INIS)

    Rous sarcoma virus (RSV) requires incomplete splicing of its viral transcripts to maintain efficient replication. A splicing inhibitor element, the negative regulator of splicing (NRS), is located near the 5' end of the RNA but the significance of this positioning is not known. In a heterologous intron the NRS functions optimally when positioned close to the authentic 5' splice site. This observation led us to investigate the basis of the position dependence. Four explanations were put forth and stressed the role of three major elements involved in splicing, the 3' splice site, the 5' splice site, and the 5' end cap structure. NRS function was unrelated to its position relative to the 3' splice site or the cap structure and appeared to depend on its position relative to the authentic 5' splice site. We conclude that position dependence may reflect distance constraints necessary for competition of the NRS with the authentic 5' splice site for pairing with the 3' splice sites

  1. Highest Oxygen Bar

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The world’s highest altitude Lhalu Wetland in Tibet is rebounding from past environmental damage In Lhasa, where the oxygen content is just 60 percent of that of the plain area, a place known as the "natural oxygen bar"is highly prized by residents.

  2. The IPG BAR project

    NARCIS (Netherlands)

    Mentink, B.; Henriquez, L.; Van Niekerk, L.; Verheul, R.

    2013-01-01

    The booklets 'Making Patterns' and 'Using Patterns' describe two aspects of the the pattern method within the urban design process. The accompanying IPG BAR Pattern Library is a small pattern library of urban-airport symbiosis patterns. These documents are the result of the Interdisciplinary Projec

  3. Raising the Bar (3)

    NARCIS (Netherlands)

    Elhorst, P.; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    2016-01-01

    This editorial summarizes and comments on the papers published in issue 11(3) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper proposes spatial and a-spatial indicators to describe the networks of airline companies around the world. The second pap

  4. Estimation of City Bus Travelers Using GSM Network

    Directory of Open Access Journals (Sweden)

    Shri Mukta Ranjan Singha,

    2013-04-01

    Full Text Available The mobile phone connectivity and its transition record can be used as useful information to estimate traffic users on urban roads. Mandatory use of a mobile phone dedicated to the city bus, can help in finding all other mobile phone users who are moving in the same city bus. Because, all the mobile phones will have same transition records with the mobile phone dedicated to the city bus. With this arrangement, at the background and Mobile Phone Network as a background data collection system, we have developed an algorithm to estimate the number city bus on an urban road and around a road junction at a particular time. The algorithm will also show the number of city bus users on urban road and around an urban road junction at a particular time. This estimate will help the urban traffic managers to optimize the city bus flow to minimize traffic congestion.

  5. Reliability analysis of the Angra-1 safety electric bus bar, considering the new Diesel generators configuration

    International Nuclear Information System (INIS)

    Aiming the electrical system reliability improvement, the Angra-1 energy electric system has been modified. The two original Diesel generators were replaced by two new ones, and the former were configured as standby generators. The purpose of this work is to quantify the electric system reliability improvement under the mentioned modifications by using Markovian analysis. It was found that the new configuration for the emergency Diesel system improves significantly the power supply to the safety buses. (author). 8 refs., 3 figs., 5 tabs

  6. Emission inventory estimation of an intercity bus terminal.

    Science.gov (United States)

    Qiu, Zhaowen; Li, Xiaoxia; Hao, Yanzhao; Deng, Shunxi; Gao, H Oliver

    2016-06-01

    Intercity bus terminals are hotspots of air pollution due to concentrated activities of diesel buses. In order to evaluate the bus terminals' impact on air quality, it is necessary to estimate the associated mobile emission inventories. Since the vehicles' operating condition at the bus terminal varies significantly, conventional calculation of the emissions based on average emission factors suffers the loss of accuracy. In this study, we examined a typical intercity bus terminal-the Southern City Bus Station of Xi'an, China-using a multi-scale emission model-(US EPA's MOVES model)-to quantity the vehicle emission inventory. A representative operating cycle for buses within the station is constructed. The emission inventory was then estimated using detailed inputs including vehicle ages, operating speeds, operating schedules, and operating mode distribution, as well as meteorological data (temperature and humidity). Five functional areas (bus yard, platforms, disembarking area, bus travel routes within the station, and bus entrance/exit routes) at the terminal were identified, and the bus operation cycle was established using the micro-trip cycle construction method. Results of our case study showed that switching to compressed natural gas (CNG) from diesel fuel could reduce PM2.5 and CO emissions by 85.64 and 6.21 %, respectively, in the microenvironment of the bus terminal. When CNG is used, tail pipe exhaust PM2.5 emission is significantly reduced, even less than brake wear PM2.5. The estimated bus operating cycles can also offer researchers and policy makers important information for emission evaluation in the planning and design of any typical intercity bus terminals of a similar scale. PMID:27220506

  7. Study of bus driving parameters on urban route

    OpenAIRE

    Fuad DASHDAMIROV

    2011-01-01

    The article deals with bus traffic time and speed on stage and route in the whole. The factors affecting bus driving parameters are divided into groups. The factors of significant impact are found in each group. The method of consideration of such factors impact on driving parameters in timetable making, is proposed. The extent of impact of selected factors on bus driving time and speed is studied.

  8. TMS communications hardware. Volume 2: Bus interface unit

    Science.gov (United States)

    Brown, J. S.; Hopkins, G. T.

    1979-01-01

    A prototype coaxial cable bus communication system used in the Trend Monitoring System to interconnect intelligent graphics terminals to a host minicomputer is described. The terminals and host are connected to the bus through a microprocessor-based RF modem termed a Bus Interface Unit (BIU). The BIU hardware and the Carrier Sense Multiple Access Listen-While-Talk protocol used on the network are described.

  9. Aberrant splicing and drug resistance in AML.

    Science.gov (United States)

    de Necochea-Campion, Rosalia; Shouse, Geoffrey P; Zhou, Qi; Mirshahidi, Saied; Chen, Chien-Shing

    2016-01-01

    The advent of next-generation sequencing technologies has unveiled a new window into the heterogeneity of acute myeloid leukemia (AML). In particular, recurrent mutations in spliceosome machinery and genome-wide aberrant splicing events have been recognized as a prominent component of this disease. This review will focus on how these factors influence drug resistance through altered splicing of tumor suppressor and oncogenes and dysregulation of the apoptotic signaling network. A better understanding of these factors in disease progression is necessary to design appropriate therapeutic strategies recognizing specific alternatively spliced or mutated oncogenic targets. PMID:27613060

  10. ASDB: database of alternatively spliced genes

    OpenAIRE

    Dralyuk, I; Brudno, M.; Gelfand, M S; Zorn, M.; Dubchak, I.

    2000-01-01

    Version 2.1 of ASDB (Alternative Splicing Data Base) contains 1922 protein and 2486 DNA sequences. The protein entries from SWISS-PROT are joined into clusters corresponding to alternatively spliced variants of one gene. The DNA division consists of complete genes with alternative splicing mentioned or annotated in GenBank. The search engine allows one to search over SWISS-PROT and GenBank fields and then follow the links to all variants. The database can be assessed at the URL http://cbcg.ne...

  11. Regulation of Alternative Splicing in Vivo by Overexpression of Antagonistic Splicing Factors

    Science.gov (United States)

    Caceres, Javier F.; Stamm, Stefan; Helfman, David M.; Krainer, Adrian R.

    1994-09-01

    The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.

  12. Observations of offshore bar decay

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian; Hughes, Michael G

    2010-01-01

    the upper shoreface, and finally a stage of decaying bar form through loss of sediment volume at the outer boundary of the upper shoreface. The phenomenon has been previously documented in the Netherlands, the USA, the Canadian Great Lakes, and in New Zealand, but our present understanding of the...... morphodynamic processes and sediment transport pathways involved in bar decay is limited. In this paper, long-term, net offshore bar migration is investigated at Vejers Beach, located on the North Sea coast of Denmark where offshore bar migration rates are of the order of 45–55 m a-1. A wave height...... transformation model confirmed that the decay of the outer bar results in increased wave heights and undertow speeds at the more landward bar potentially causing this bar to speed up its offshore migration. The causes for outer bar decay were investigated through field measurements of sediment transport at the...

  13. Efficient multiqubit entanglement via a spin-bus

    CERN Document Server

    Friesen, M; Hu, X; Lidar, D; Biswas, Asoka; Friesen, Mark; Hu, Xuedong; Lidar, Daniel

    2007-01-01

    We propose an experimentally feasible architecture where controllable long-range couplings between spins are built up from local exchange interactions. The scheme consists of a spin-bus, with strong, always-on interactions, coupled dynamically to external spin qubits. The presence of a spectral gap enables the bus to function as a long-range conduit for spin information. The bus also forms a hub for multiqubit entangling operations. We show how multiqubit gates may be used to efficiently generate $W$-states--an important entanglement resource. In this way, the spin-bus provides an alternate route for scalable solid-state quantum computation, using currently available experimental resources.

  14. Status of the IEEE P896 Future Backplane Bus

    International Nuclear Information System (INIS)

    The IEEE P896 Future Backplane Bus project has been influenced by and has influenced FASTBUS and several other contemporary bus designs. This paper summarizes the current status of that project, which is directed toward the needs of modern 32-bit microprocessor systems with multiple processors. Some of the technology developed for P896 will be important for future non-ECL implementations of FASTBUS and other buses. In particular, new bus drivers and receivers should greatly improve the performance and reliability of backplane buses and cable buses. The current status of the P896 serial bus is also summarized

  15. Examination of an Urban City Bus Operating Conditions and Emissions

    OpenAIRE

    Soylu, Seref (Coordinador); Bal, Ayda; Semercioglu, Hulya; Ay, Eyup Fatih

    2010-01-01

    It was observed that real world driving characteristics of the city bus don’t correlate well with that of the ETC, which is the certification cycle for the city bus engines. For this reason it is not realistic to expect emission levels lower than corresponding EURO standards from the city bus during real world driving. City bus NOx emissions in terms of grams per second are increasing with engine load and speed but the total NOx emissions for entire trip are the maximum at the locations whe...

  16. The Software Bus, an Object-Oriented Data Exchange System

    International Nuclear Information System (INIS)

    This document describes the Software Bus System, developed for object-oriented task to task communication in a TCP/IP based network. The Software Bus is a set of library functions, developed to be used for the Picasso-3 UIMS, and as a general purpose tool for dynamically interfacing programs at run-time. The Software Bus offers a high level object-oriented data exchange mechanism that relieves the application programmer of the low level TCP/IP-programming and communication protocol handling. The Software Bus is currently available under several UNIX platforms and a version for Windows NT is planned for late 1996. (author)

  17. Multi-Gigabit Rate Radiation Hard Bus Project

    Data.gov (United States)

    National Aeronautics and Space Administration — High speed robust very low power radiation hardened bus interconnects are required to accelerate on-board computations, further improve reconfigurability and...

  18. Analysis of differential splicing suggests different modes of short-term splicing regulation

    OpenAIRE

    Topa, Hande; Honkela, Antti

    2016-01-01

    Motivation: Alternative splicing is an important mechanism in which the regions of pre-mRNAs are differentially joined in order to form different transcript isoforms. Alternative splicing is involved in the regulation of normal physiological functions but also linked to the development of diseases such as cancer. We analyse differential expression and splicing using RNA-sequencing time series in three different settings: overall gene expression levels, absolute transcript expression levels an...

  19. Implementation of viscoelastic Hopkinson bars

    OpenAIRE

    Govender R; Cloete T.; Curry R.

    2012-01-01

    Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic na...

  20. Hollywood: a comparative relational database of alternative splicing

    OpenAIRE

    Holste, Dirk; Huo, George; Tung, Vivian; Burge, Christopher B.

    2005-01-01

    RNA splicing is an essential step in gene expression, and is often variable, giving rise to multiple alternatively spliced mRNA and protein isoforms from a single gene locus. The design of effective databases to support experimental and computational investigations of alternative splicing (AS) is a significant challenge. In an effort to integrate accurate exon and splice site annotation with current knowledge about splicing regulatory elements and predicted AS events, and to link information ...

  1. HIV-1 Vpr: A Novel Role in Regulating RNA Splicing

    OpenAIRE

    Zhang, Xianfeng; Aida, Yoko

    2009-01-01

    Pre-mRNA splicing is a critical step in gene expression for metazoans. Several viral proteins regulate the splicing of pre-mRNAs through complex interactions between the virus and the host cell RNA splicing machinery. Here, we focus on a novel function of HIV-1 Vpr, that selectively inhibit cellular and viral pre-mRNA splicing, via interactions with components of functional spliceosomal complexes. This review discusses our current knowledge of how RNA splicing regulation is accomplished by Vp...

  2. Control of Pre-mRNA Splicing by the General Splicing Factors PUF60 and U2AF65

    OpenAIRE

    Hastings, Michelle L.; Eric Allemand; Duelli, Dominik M.; Michael P Myers; Krainer, Adrian R.

    2007-01-01

    Pre-mRNA splicing is a crucial step in gene expression, and accurate recognition of splice sites is an essential part of this process. Splice sites with weak matches to the consensus sequences are common, though it is not clear how such sites are efficiently utilized. Using an in vitro splicing-complementation approach, we identified PUF60 as a factor that promotes splicing of an intron with a weak 3' splice-site. PUF60 has homology to U2AF(65), a general splicing factor that facilitates 3' s...

  3. A Novel Type of Splicing Enhancer Regulating Adenovirus Pre-mRNA Splicing

    OpenAIRE

    Mühlemann, Oliver; Yue, Bai-Gong; Petersen-Mahrt, Svend; Akusjärvi, Göran

    2000-01-01

    Splicing of the adenovirus IIIa pre-mRNA is subjected to a temporal regulation, such that efficient IIIa 3′ splice site usage is confined to the late phase of the infectious cycle. Here we show that IIIa pre-mRNA splicing is activated more than 200-fold in nuclear extracts prepared from late adenovirus-infected cells (Ad-NE) compared to uninfected HeLa cell nuclear extracts (HeLa-NE). In contrast, splicing of the β-globin pre-mRNA is repressed in Ad-NE. We constructed hybrid pre-mRNAs between...

  4. Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing.

    Science.gov (United States)

    Munding, Elizabeth M; Shiue, Lily; Katzman, Sol; Donohue, John Paul; Ares, Manuel

    2013-08-01

    During meiosis in yeast, global splicing efficiency increases and then decreases. Here we provide evidence that splicing improves due to reduced competition for the splicing machinery. The timing of this regulation corresponds to repression and reactivation of ribosomal protein genes (RPGs) during meiosis. In vegetative cells, RPG repression by rapamycin treatment also increases splicing efficiency. Downregulation of the RPG-dedicated transcription factor gene IFH1 genetically suppresses two spliceosome mutations, prp11-1 and prp4-1, and globally restores splicing efficiency in prp4-1 cells. We conclude that the splicing apparatus is limiting and that pre-messenger RNAs compete. Splicing efficiency of a pre-mRNA therefore depends not just on its own concentration and affinity for limiting splicing factor(s), but also on those of competing pre-mRNAs. Competition between RNAs for limiting processing factors appears to be a general condition in eukaryotes for a variety of posttranscriptional control mechanisms including microRNA (miRNA) repression, polyadenylation, and splicing. PMID:23891561

  5. Dark Matter Trapping by Stellar Bars: The Shadow Bar

    CERN Document Server

    Petersen, Michael S; Katz, Neal

    2016-01-01

    We investigate the complex interactions between the stellar disc and the dark-matter halo during bar formation and evolution using N-body simulations with fine temporal resolution and optimally chosen spatial resolution. We find that the forming stellar bar traps dark matter in the vicinity of the stellar bar into bar-supporting orbits. We call this feature the shadow bar. The shadow bar modifies both the location and magnitude of the angular momentum transfer between the disc and dark matter halo and adds 10 per cent to the mass of the stellar bar over 4 Gyr. The shadow bar is potentially observable by its density and velocity signature in spheroid stars and by direct dark matter detection experiments. Numerical tests demonstrate that the shadow bar can diminish the rate of angular momentum transport from the bar to the dark matter halo by more than a factor of three over the naive dynamical friction prediction, and thus provides a possible physical explanation for the observed prevalence of fast bars in nat...

  6. Dark Matter Trapping by Stellar Bars: The Shadow Bar

    Science.gov (United States)

    Petersen, Michael S.; Weinberg, Martin D.; Katz, Neal

    2016-09-01

    We investigate the complex interactions between the stellar disc and the dark-matter halo during bar formation and evolution using N-body simulations with fine temporal resolution and optimally chosen spatial resolution. We find that the forming stellar bar traps dark matter in the vicinity of the stellar bar into bar-supporting orbits. We call this feature the shadow bar. The shadow bar modifies both the location and magnitude of the angular momentum transfer between the disc and dark matter halo and adds 10 per cent to the mass of the stellar bar over 4 Gyr. The shadow bar is potentially observable by its density and velocity signature in spheroid stars and by direct dark matter detection experiments. Numerical tests demonstrate that the shadow bar can diminish the rate of angular momentum transport from the bar to the dark matter halo by more than a factor of three over the rate predicted by dynamical friction with an untrapped dark halo, and thus provides a possible physical explanation for the observed prevalence of fast bars in nature.

  7. 76 FR 37393 - FY 2011 Discretionary Livability Funding Opportunity; Section 5309 Bus and Bus Facilities...

    Science.gov (United States)

    2011-06-27

    ... project includes elements to conserve energy, such as passive solar heating, solar panels, wind turbines... (49 U.S.C. 5339), both authorized by the Safe, Accountable, Flexible, Efficient, Transportation Equity... Bus Facilities Program funds, authorized by 49 U.S.C. 5309(b) of the Safe, Accountable,...

  8. Cooling of rectangular bars

    International Nuclear Information System (INIS)

    A solution of the time-transient Heat Transfer Differential Equation in rectangular coordinates is presented, leading to a model which describes the temperature drop with time in rectangular bars. It is similar to an other model for cilindrical bars which has been previously developed in the Laboratory of Mechanical Metallurgy of UFRGS. Following these models, a generalization has been made, which permits cooling time evaluation for all profiles. These results are compared with experimental laboratory data in the 1200 to 8000C range. Some other existing models were also studied which have the purpose of studing the same phenomenon. Their mathematical forms and their evaluated values are analyzed and compared with experimental ones. (Author)

  9. The bar instability revisited

    CERN Document Server

    Chiodi, Filippo; Claudin, Philippe

    2012-01-01

    The river bar instability is revisited, using a hydrodynamical model based on Reynolds averaged Navier-Stokes equations. The results are contrasted with the standard analysis based on shallow water Saint-Venant equations. We first show that the stability of both transverse modes (ripples) and of small wavelength inclined modes (bars) predicted by the Saint-Venant approach are artefacts of this hydrodynamical approximation. When using a more reliable hydrodynamical model, the dispersion relation does not present any maximum of the growth rate when the sediment transport is assumed to be locally saturated. The analysis therefore reveals the fundamental importance of the relaxation of sediment transport towards equilibrium as it it is responsible for the stabilisation of small wavelength modes. This dynamical mechanism is characterised by the saturation number, defined as the ratio of the saturation length to the water depth Lsat/H. This dimensionless number controls the transition from ripples (transverse patte...

  10. Group II Intron Self-Splicing.

    Science.gov (United States)

    Pyle, Anna Marie

    2016-07-01

    Group II introns are large, autocatalytic ribozymes that catalyze RNA splicing and retrotransposition. Splicing by group II introns plays a major role in the metabolism of plants, fungi, and yeast and contributes to genetic variation in many bacteria. Group II introns have played a major role in genome evolution, as they are likely progenitors of spliceosomal introns, retroelements, and other machinery that controls genetic variation and stability. The structure and catalytic mechanism of group II introns have recently been elucidated through a combination of genetics, chemical biology, solution biochemistry, and crystallography. These studies reveal a dynamic machine that cycles progressively through multiple conformations as it stimulates the various stages of splicing. A central active site, containing a reactive metal ion cluster, catalyzes both steps of self-splicing. These studies provide insights into RNA structure, folding, and catalysis, as they raise new questions about the behavior of RNA machines. PMID:27391926

  11. Faster exon assembly by sparse spliced alignment

    CERN Document Server

    Tiskin, Alexander

    2007-01-01

    Assembling a gene from candidate exons is an important problem in computational biology. Among the most successful approaches to this problem is \\emph{spliced alignment}, proposed by Gelfand et al., which scores different candidate exon chains within a DNA sequence of length $m$ by comparing them to a known related gene sequence of length n, $m = \\Theta(n)$. Gelfand et al.\\ gave an algorithm for spliced alignment running in time O(n^3). Kent et al.\\ considered sparse spliced alignment, where the number of candidate exons is O(n), and proposed an algorithm for this problem running in time O(n^{2.5}). We improve on this result, by proposing an algorithm for sparse spliced alignment running in time O(n^{2.25}). Our approach is based on a new framework of \\emph{quasi-local string comparison}.

  12. Protein splicing and its evolution in eukaryotes

    Directory of Open Access Journals (Sweden)

    Starokadomskyy P. L.

    2010-02-01

    Full Text Available Inteins, or protein introns, are parts of protein sequences that are post-translationally excised, their flanking regions (exteins being spliced together. This process was called protein splicing. Originally inteins were found in prokaryotic or unicellular eukaryotic organisms. But the general principles of post-translation protein rearrangement are evolving yielding different post-translation modification of proteins in multicellular organisms. For clarity, these non-intein mediated events call either protein rearrangements or protein editing. The most intriguing example of protein editing is proteasome-mediated splicing of antigens in vertebrates that may play important role in antigen presentation. Other examples of protein rearrangements are maturation of Hg-proteins (critical receptors in embryogenesis as well as maturation of several metabolic enzymes. Despite a lack of experimental data we try to analyze some intriguing examples of protein splicing evolution.

  13. Oracle Service Bus 11g Development Cookbook

    CERN Document Server

    Schmutz, Guido; van Zoggel, Jan

    2012-01-01

    This cookbook is full of immediately useable recipes showing you how to develop service and message-oriented (integration) applications on the Oracle Service Bus. In addition to its cookbook style, which ensures the solutions are presented in a clear step-by-step manner, the explanations go into great detail, which makes it good learning material for everyone who has experience in OSB and wants to improve. Most of the recipes are designed in such a way that each recipe is presented as a separate, standalone entity and reading of prior recipes is not required. The finished solution of each reci

  14. Availability of ARINC 629 Avionic Data Bus

    Directory of Open Access Journals (Sweden)

    Alban Gabillon

    2006-12-01

    Full Text Available The three traditional objectives of computer security are confidentiality, integrity and availability. Availability can be defined as the prevention of denial of service. Confidentiality and integrity have been addressed in several theoretical works whereas the concept of availability has not been much investigated by the scientific community. This paper is an attempt to define through a case study the concept of availability. We first define a set of availability constraints that avionic data bus protocols should enforce. Then, we consider the ARINC 629 Basic Protocol (BP and the ARINC Combined Protocol (CP which were implemented on the Boeing 777. We check whether these protocols respect our availability constraints or not.

  15. Redesign of Transjakarta Bus Driver's Cabin

    Science.gov (United States)

    Mardi Safitri, Dian; Azmi, Nora; Singh, Gurbinder; Astuti, Pudji

    2016-02-01

    Ergonomic risk at work stations with type Seated Work Control was one of the problems faced by Transjakarta bus driver. Currently “Trisakti” type bus, one type of bus that is used by Transjakarta in corridor 9, serving route Pinang Ranti - Pluit, gained many complaints from drivers. From the results of Nordic Body Map questionnaires given to 30 drivers, it was known that drivers feel pain in the neck, arms, hips, and buttocks. Allegedly this was due to the seat position and the button/panel bus has a considerable distance range (1 meter) to be achieved by drivers. In addition, preliminary results of the questionnaire using Workstation Checklist identified their complaints about uncomfortable cushion, driver's seat backrest, and the exact position of the AC is above the driver head. To reduce the risk level of ergonomics, then did research to design the cabin by using a generic approach to designing products. The risk analysis driver posture before the design was done by using Rapid Upper Limb Assessment (RULA), Rapid Entire Body Assessment (REBA), and Quick Exposure Checklist (QEC), while the calculation of the moment the body is done by using software Mannequin Pro V10.2. Furthermore, the design of generic products was done through the stages: need metric-matrix, house of quality, anthropometric data collection, classification tree concept, concept screening, scoring concept, design and manufacture of products in the form of two-dimensional. While the design after design risk analysis driver posture was done by using RULA, REBA, and calculation of moments body as well as the design visualized using software 3DMax. From the results of analysis before the draft design improvements cabin RULA obtained scores of 6, REBA 9, and the result amounted to 57.38% QEC and moment forces on the back is 247.3 LbF.inch and on the right hip is 72.9 LbF.in. While the results of the proposed improvements cabin design RULA obtained scores of 3, REBA 4, and the moment of force on

  16. The Possible Heavy Tetraquarks $qQ\\bar q \\bar Q$, $qq\\bar Q \\bar Q$ and $qQ\\bar Q \\bar Q$

    OpenAIRE

    CUI, Ying; Chen, Xiao-Lin; Deng, Wei-Zhen; Zhu, Shi-Lin

    2006-01-01

    Assuming X(3872) is a $qc \\bar q \\bar c$ tetraquark and using its mass as input, we perform a schematic study of the masses of possible heavy tetraquarks using the color-magnetic interaction with the flavor symmetry breaking corrections.

  17. Tau exon 10 alternative splicing and tauopathies

    OpenAIRE

    Liu Fei; Gong Cheng-Xin

    2008-01-01

    Abstract Abnormalities of microtubule-associated protein tau play a central role in neurofibrillary degeneration in several neurodegenerative disorders that collectively called tauopathies. Six isoforms of tau are expressed in adult human brain, which result from alternative splicing of pre-mRNA generated from a single tau gene. Alternative splicing of tau exon 10 results in tau isoforms containing either three or four microtubule-binding repeats (3R-tau and 4R-tau, respectively). Approximate...

  18. Splice Junction Map of Simian Parvovirus Transcripts

    OpenAIRE

    Vashisht, Kapil; Faaberg, Kay S.; Aber, Amanda L.; Brown, Kevin E.; O’Sullivan, M. Gerard

    2004-01-01

    The transcription map of simian parvovirus (SPV), an Erythrovirus similar to Parvovirus B19, was investigated. RNA was extracted from tissues of experimentally infected cynomolgus macaques and subjected to reverse transcription-PCR with SPV-specific primers. The PCR products were cloned and sequenced to identify splice junctions. A total of 14 distinct sequences were identified as putative partial transcripts. Of these, 13 were spliced; a single unspliced transcript putatively encoded NS1. Se...

  19. Alcoholism and Alternative Splicing of Candidate Genes

    OpenAIRE

    Toshikazu Sasabe; Shoichi Ishiura

    2010-01-01

    Gene expression studies have shown that expression patterns of several genes have changed during the development of alcoholism. Gene expression is regulated not only at the level of transcription but also through alternative splicing of pre-mRNA. In this review, we discuss some of the evidence suggesting that alternative splicing of candidate genes such as DRD2 (encoding dopamine D2 receptor) may form the basis of the mechanisms underlying the pathophysiology of alcoholism. These reports sugg...

  20. Djungle power. A more remote AC bus

    Energy Technology Data Exchange (ETDEWEB)

    Boehnke, Heinz W. [Technosol, Jork (Germany)

    2010-07-01

    The Dzanga-Sangha Reserve in the Central African Republic is probably one of the world's most remote protected rainforest areas, much to the benefit of the rare species of lowland gorilla, forest elephant and local pygmy population Ba'Aka. The park is co-managed by the ''WWF-World Wide Fund for Nature'' with sponsoring from the German Regenwald-Stiftung. In 2008 wwf decided to switch from the old, failing generator supply to a solar hybrid mini-grid, along with stringent measures of energy conservation and management. After a careful energy audit Technosol designed an AC-bus system with a 22 kWp PV generator which should overcome the dependency on the old generators and their fuel demands. For 15 buildings, an entirely new distribution network was installed, state of the art energy saving devices introduced and an energy metering system deviced. The installation is operating since August 2009. In such remote locations, the reliability of the AC-bus over other generator-based solutions will be demonstrated and with the support of international organisations like the wwf the practical application of solar hybrid supply becomes a landmark of sustainable energy concepts. (orig.)

  1. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates

    OpenAIRE

    Manuel Irimia; Jakob Lewin Rukov; Scott William Roy

    2009-01-01

    Alternative splicing is tightly regulated in a spatio-temporal and quantitative manner. This regulation is achieved by a complex interplay between spliceosomal (trans) factors that bind to different sequence (cis) elements. cis-elements reside in both introns and exons and may either enhance or silence splicing. Differential combinations of cis-elements allows for a huge diversity of overall splicing signals, together comprising a complex ‘splicing code’. Many cis-elements have been identifie...

  2. Network Analysis of Urban Traffic with Big Bus Data

    CERN Document Server

    Zhao, Kai

    2016-01-01

    Urban traffic analysis is crucial for traffic forecasting systems, urban planning and, more recently, various mobile and network applications. In this paper, we analyse urban traffic with network and statistical methods. Our analysis is based on one big bus dataset containing 45 million bus arrival samples in Helsinki. We mainly address following questions: 1. How can we identify the areas that cause most of the traffic in the city? 2. Why there is a urban traffic? Is bus traffic a key cause of the urban traffic? 3. How can we improve the urban traffic systems? To answer these questions, first, the betweenness is used to identify the most import areas that cause most traffics. Second, we find that bus traffic is not an important cause of urban traffic using statistical methods. We differentiate the urban traffic and the bus traffic in a city. We use bus delay as an identification of the urban traffic, and the number of bus as an identification of the bus traffic. Third, we give our solutions on how to improve...

  3. Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus: Preliminary Evaluation Results

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K.; Eudy, L.

    2008-10-01

    This report provides preliminary results from a National Renewable Energy Laboratory evaluation of a protoptye fuel cell transit bus operating at Connecticut Transit in Hartford. Included are descriptions of the planned fuel cell bus demonstration and equipment; early results and agency experience are also provided.

  4. A decentralized software bus based on IP multicas ting

    Science.gov (United States)

    Callahan, John R.; Montgomery, Todd

    1995-01-01

    We describe decentralized reconfigurable implementation of a conference management system based on the low-level Internet Protocol (IP) multicasting protocol. IP multicasting allows low-cost, world-wide, two-way transmission of data between large numbers of conferencing participants through the Multicasting Backbone (MBone). Each conference is structured as a software bus -- a messaging system that provides a run-time interconnection model that acts as a separate agent (i.e., the bus) for routing, queuing, and delivering messages between distributed programs. Unlike the client-server interconnection model, the software bus model provides a level of indirection that enhances the flexibility and reconfigurability of a distributed system. Current software bus implementations like POLYLITH, however, rely on a centralized bus process and point-to-point protocols (i.e., TCP/IP) to route, queue, and deliver messages. We implement a software bus called the MULTIBUS that relies on a separate process only for routing and uses a reliable IP multicasting protocol for delivery of messages. The use of multicasting means that interconnections are independent of IP machine addresses. This approach allows reconfiguration of bus participants during system execution without notifying other participants of new IP addresses. The use of IP multicasting also permits an economy of scale in the number of participants. We describe the MULITIBUS protocol elements and show how our implementation performs better than centralized bus implementations.

  5. Review of bus drivers' occupational stress and stress prevention

    NARCIS (Netherlands)

    Kompier, M.A.J.; Di Martino, V.

    1995-01-01

    Although heterogeneous in methodology and content, 32 studies from 13 countries on bus drivers' work and health are similar in their conclusions. Bus-driving–characterized by high demands, low control and low support – can be regarded as a classic example of high-strain occupation, with high risks o

  6. Bus accident severity and passenger injury: evidence from Denmark

    DEFF Research Database (Denmark)

    Prato, Carlo Giacomo; Kaplan, Sigal

    2014-01-01

    principle of sustainable transit and advance the vision “every accident is one too many”. Methods Bus accident data were retrieved from the national accident database for the period 2002–2011. A generalized ordered logit model allows analyzing bus accident severity and a logistic regression enables...

  7. Splicing therapy for neuromuscular disease.

    Science.gov (United States)

    Douglas, Andrew G L; Wood, Matthew J A

    2013-09-01

    Duchenne muscular dystrophy (DMD) and spinal muscular atrophy (SMA) are two of the most common inherited neuromuscular diseases in humans. Both conditions are fatal and no clinically available treatments are able to significantly alter disease course in either case. However, by manipulation of pre-mRNA splicing using antisense oligonucleotides, defective transcripts from the DMD gene and from the SMN2 gene in SMA can be modified to once again produce protein and restore function. A large number of in vitro and in vivo studies have validated the applicability of this approach and an increasing number of preliminary clinical trials have either been completed or are under way. Several different oligonucleotide chemistries can be used for this purpose and various strategies are being developed to facilitate increased delivery efficiency and prolonged therapeutic effect. As these novel therapeutic compounds start to enter the clinical arena, attention must also be drawn to the question of how best to facilitate the clinical development of such personalised genetic therapies and how best to implement their provision. PMID:23631896

  8. Alternative Splice in Alternative Lice.

    Science.gov (United States)

    Tovar-Corona, Jaime M; Castillo-Morales, Atahualpa; Chen, Lu; Olds, Brett P; Clark, John M; Reynolds, Stuart E; Pittendrigh, Barry R; Feil, Edward J; Urrutia, Araxi O

    2015-10-01

    Genomic and transcriptomics analyses have revealed human head and body lice to be almost genetically identical; although con-specific, they nevertheless occupy distinct ecological niches and have differing feeding patterns. Most importantly, while head lice are not known to be vector competent, body lice can transmit three serious bacterial diseases; epidemictyphus, trench fever, and relapsing fever. In order to gain insights into the molecular bases for these differences, we analyzed alternative splicing (AS) using next-generation sequencing data for one strain of head lice and one strain of body lice. We identified a total of 3,598 AS events which were head or body lice specific. Exon skipping AS events were overrepresented among both head and body lice, whereas intron retention events were underrepresented in both. However, both the enrichment of exon skipping and the underrepresentation of intron retention are significantly stronger in body lice compared with head lice. Genes containing body louse-specific AS events were found to be significantly enriched for functions associated with development of the nervous system, salivary gland, trachea, and ovarian follicle cells, as well as regulation of transcription. In contrast, no functional categories were overrepresented among genes with head louse-specific AS events. Together, our results constitute the first evidence for transcript pool differences in head and body lice, providing insights into molecular adaptations that enabled human lice to adapt to clothing, and representing a powerful illustration of the pivotal role AS can play in functional adaptation. PMID:26169943

  9. Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Daniel Nilsson

    Full Text Available Trans-splicing of leader sequences onto the 5'ends of mRNAs is a widespread phenomenon in protozoa, nematodes and some chordates. Using parallel sequencing we have developed a method to simultaneously map 5'splice sites and analyze the corresponding gene expression profile, that we term spliced leader trapping (SLT. The method can be applied to any organism with a sequenced genome and trans-splicing of a conserved leader sequence. We analyzed the expression profiles and splicing patterns of bloodstream and insect forms of the parasite Trypanosoma brucei. We detected the 5' splice sites of 85% of the annotated protein-coding genes and, contrary to previous reports, found up to 40% of transcripts to be differentially expressed. Furthermore, we discovered more than 2500 alternative splicing events, many of which appear to be stage-regulated. Based on our findings we hypothesize that alternatively spliced transcripts present a new means of regulating gene expression and could potentially contribute to protein diversity in the parasite. The entire dataset can be accessed online at TriTrypDB or through: http://splicer.unibe.ch/.

  10. An Overview of Staged-bus Operation in Cities

    Directory of Open Access Journals (Sweden)

    Mohammad Hesam Hafezi

    2014-02-01

    Full Text Available In developing countries, demand for passenger travel is higher, due to economic growth. Government policy encourages people to use public transportation instead of their private carsto reduce of traffic congestion and air pollution. In the city areas, bus networks are the major part of the public transportation system because they are easily accessible and cheaper compared to other types of public transportation. In this study revelation of details of bus operation included different agents is studied. Bus operations, during provision of their service, have interaction with three main agents: bus agent, passenger agent and traffic agent. Study of the influence of each agent in bus operation is presented in this study, using the macroscopic way.

  11. Improved design of HIRFL-CSR EVME bus controller

    International Nuclear Information System (INIS)

    The EVME bus controller which is a key component of the HIRFL-CSR control system was improved. Besides reconfiguring the embedded Linux, a utility program was developed for data exchange between the controller and the database. The bus controller is based on ARM920T(ARM9) micro processor which is BGA packaged. The bus controller has the universal interface of VGA display, keyboard, and mouse. The backboard interface logic is programmed in an in-system configurable FPGA device. The bus can drive high current up to 64 mA, with the flexibility of the programmable signal definitions. All the improved performance helped the EVME bus controller play a crucial role in HIRFL-CSR control system. (authors)

  12. Research on the Coordinated Design of Bus and Taxi Station

    Directory of Open Access Journals (Sweden)

    Jiangfeng Xi

    2015-01-01

    Full Text Available Rises in the number of transit buses, bus routes, and overall traffic volume in China’s cities, coupled with interference from other transport modes, such as taxis loading and unloading passengers nearby, have led to increasing traffic delays at bus stops, which is considered one of the factors degrading service levels and traffic operations on urban roadways. This paper studies traffic characteristics at bus stops, investigates variations in delay from different types or designs of bus stops, and analyzes the impact of it on traffic capacity, the purpose of which is to propose a solution to predicting the feasibility of an integrated design of bus stops and taxi stands with the help of mathematical models and based on the objectives of optimal traffic operations and passenger transfer.

  13. A global analysis of C. elegans trans-splicing

    OpenAIRE

    Allen, Mary Ann; Hillier, LaDeana W.; Waterston, Robert H.; Blumenthal, Thomas

    2011-01-01

    Trans-splicing of one of two short leader RNAs, SL1 or SL2, occurs at the 5′ ends of pre-mRNAs of many C. elegans genes. We have exploited RNA-sequencing data from the modENCODE project to analyze the transcriptome of C. elegans for patterns of trans-splicing. Transcripts of ∼70% of genes are trans-spliced, similar to earlier estimates based on analysis of far fewer genes. The mRNAs of most trans-spliced genes are spliced to either SL1 or SL2, but most genes are not trans-spliced to both, ind...

  14. Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

    Directory of Open Access Journals (Sweden)

    Elela Sherif

    2006-01-01

    Full Text Available Abstract Background We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins. In the present study, we have tested the inhibitory activity of RNA oligonucleotides carrying different tail structures. Results We show that an oligonucleotide with a 5' tail containing the human β-globin branch site sequence inhibits the use of the 5' splice site of Bcl-xL, albeit less efficiently than a tail containing binding sites for the hnRNP A1/A2 proteins. A branch site-containing tail positioned at the 3' end of the oligonucleotide also elicited splicing inhibition but not as efficiently as a 5' tail. The interfering activity of a 3' tail was improved by adding a 5' splice site sequence next to the branch site sequence. A 3' tail carrying a Y-shaped branch structure promoted similar splicing interference. The inclusion of branch site or 5' splice site sequences in the Y-shaped 3' tail further improved splicing inhibition. Conclusion Our in vitro results indicate that a variety of tail architectures can be used to elicit splicing interference at low nanomolar concentrations, thereby broadening the scope and the potential impact of this antisense technology.

  15. Work fatigue in urban bus drivers

    Directory of Open Access Journals (Sweden)

    Teresa Makowiec-Dąbrowska

    2015-10-01

    Full Text Available Background: Bus drivers are a special group of professional drivers who are at a very high risk of fatigue. The aim of the study was to examine whether the driver’s subjective assessment of fatigue allows for the determination of its level and identification of its causes. Material and Methods: The study group comprised 45 randomly selected bus drivers (mean age – 43.7±7.9 years, period of employment as drivers – 14.7±8.6 years. Examinations were performed in all subjects four times – before and after work on the “easy” route (outside the city center, small traffic intensity and before and after work on the “difficult” route (city center, heavy traffic. The fatigue test questionnaire, based on the list of symptoms of fatigue prepared by the Japan Research Committee of Fatigue, was used in the study. Results: The rating of fatigue after the work was significantly higher than that before the work. The profile of fatigue after work was not influenced by the type of route, but the assessment of most symptoms of fatigue reached a higher level after the “difficult” routes and the differences were statistically significant for 7 symptoms. Only the ratings of leg fatigue, feeling of heaviness, and the necessity to squint eyes and gaze with effort reached the higher levels after driving the “easy” routes. It has been found that the level of fatigue was significantly correlated with the job characteristics (driving time, the length of the route, number of stops, etc. and with the abundance of food ingested and type of beverage (coffee vs. others drunk prior to driving. Conclusions: The questionnaire used in our study to assess the subjective feeling of fatigue has proved to be a sensitive and useful tool for indicating the level and causes of fatigue. The relationship between the symptoms of fatigue and the characteristics of job and lifestyle shows that actions must be taken by both the employers and employees to prevent fatigue

  16. Influence of steel fibres on bond and development length of deformed bars in normal strength concrete

    Science.gov (United States)

    Tenzey, Ugyen

    Transverse reinforcement (stirrups) plays an important role in improving bond and anchorage of deformed bars in reinforced concrete structures. Steel fibres or steel fibre reinforced concrete (SFRC) can be used in lieu of stirrups to provide a similar beneficial effect. The application of steel fibres in lieu of stirrups is not recognized in codes of practice for concrete structures because of limited research for this type of application. The results of this study are based on 18 large scale test beams (250 mm wide by 300 mm high and 3.4 m long). Control cylinders and flexure prisms are used to obtain the required concrete material properties together with tension tests of the steel rebar. The focus of this research is to investigate the influence of steel fibres to enhance bond and development of deformed reinforcing bars in normal strength reinforced concrete beams. An attempt is also made to develop an understanding and rationale of the effect SFRC has on improving bond. Longitudinal reinforcement in most of the beams is lap spliced with different types of confinement in the spliced region (plain concrete, plain concrete with stirrups, SFRC, and SFRC with stirrups), and evaluated under third point loading to ensure the spliced bars are subjected to a constant tensile force in the region of constant moment. All of the beams with spliced reinforcement failed in bond before yielding of the longitudinal reinforcement. The SFRC mix uses steel fibres at an 80 kg/m3 dosage (1% by volume). The plain concrete beams without any transverse reinforcement failed suddenly without any warning. The presence of steel fibres did not affect the flexural cracking load of the specimens, but did provide a consistent increase in the load capacity at bond failure and ensure a more controlled failure. The spliced beams with SFRC exhibited a 22.5% increase in the bond failure load capacity compared with the plain concrete beams. The combined effect of fibres and transverse reinforcement

  17. Status of the FASTBUS standard data bus

    International Nuclear Information System (INIS)

    FASTBUS is a new laboratory standard data bus intended for use in experimental data acquisition and control. The development of FASTBUS began with a feasibility study in 1976, and is presently at the development prototyping stage. The principal aims of the standard are to provide a factor of ten or more improvement in speed of data transfers over present systems, as well as to provide an architecture for systems containing multiple processors. Developmental prototypes now in progress include crates, backplanes, cooling devices, power supplies, test and diagnostic modules, and user modules. A sizeable software development effort is also underway. Several experiments have made commitments to use FASTBUS. A review of current work and potential applications is given. 10 figures

  18. 改进的多母线船舶电力系统短路电流计算方法%An Improved Short-Circuit Current Calculation Method for Multi-Bus Ship Power System

    Institute of Scientific and Technical Information of China (English)

    张志; 彭晓涛; 李晓宁; 吴振辉; 孙元章

    2013-01-01

    To facilitate engineering calculation and improve computational accuracy, based on traditional short-circuit calculation methods a main bus-bar based improved algorithm for multi-bus ship power system is proposed. The basic thinking of the proposed algorithm lies in following:when short-circuit fault occurred at one of generator sides, through the equivalence the cable impedances between bus-bars is transferred to generator branch and motor branches connected to opposite bus-bar, thus the influence of main bus-bar on short-circuit current is incorporated in the current that is fed from generator to the short-circuit point. The proposed improved algorithm is simulated by PSCAD, and the simulation results are compared with calculation results by traditional short-circuit current calculation method, and comparison result shows that the proposed calculation method is accurate and effective.%  为便于工程计算,提高计算精度,在传统短路电流计算方法的基础上,提出了基于多母线主汇流排的改进算法。通过对母线间电缆阻抗等效,再将其转移至故障点对侧母线的发电机支路和电动机支路,将其对短路电流的影响归于由发电机馈送至短路点的电流。通过与PSCAD仿真环境下的结果进行比较,证明了改进算法的正确性和有效性。

  19. Dynamical Evolution of Barred Galaxies

    OpenAIRE

    Athanassoula, E

    2005-01-01

    Angular momentum redistribution within barred galaxies drives their dynamical evolution. Angular momentum is emitted mainly by near-resonant material in the bar region and absorbed by resonant material mainly in the outer disc and in the halo. This exchange determines the strength of the bar, the decrease of its pattern speed, as well as its morphology. If the galaxy has also a gaseous component and/or a companion or satellite, then these also take part in the angular momentum exchange. Durin...

  20. Project survey for trolley-bus transport; Johdinautoliikenteen hankeselvitys

    Energy Technology Data Exchange (ETDEWEB)

    Anttila, T.; Eklund, P.; Airaksinen, S.; Maettoe, R.; Laehdetie, A.

    2011-04-15

    To investigate the feasibility and rationality of the revival of trolley bus traffic in Helsinki a full size project plan was conducted for a variety of alternatives. This included a survey on the latest developments of the trolley bus technology, three optional plans for route network, a budgetary level design for a fully functional depot in Koskela bus depot, examination of urban landscape structures, a study on organisational options, particular technical specifications applicable on suitable trolley buses and an examination of the impacts on the environment and on the future development of public transport. The basic concept of a trolley bus as a means of electric public transport vehicle has found its form over the years, but all the components of the system undergo at present intensive development. In context of this survey also the option of a fully autonomous electric bus powered by accumulators or fuel cells and the like was investigated. However, such technology is still totally inadequate for a heavy duty bus. There are no economically competitive and effective systems available as yet. The trolley bu is the far most reliable system and gives the best life cycle economy for a foreseeable future. Earliest estimates for alternative electric technologies in regular bus service are viable just after one investment cycle some time beyond 2030. Then it should be possible to run without the support of a comprehensive overhead line. The latest achievements in the development of trolley bus are auxiliary engine and accumulator systems allowing for more autonomous propulsion. Nowadays, trolley buses are equipped with an auxiliary power unit that alleviates the traditional restrictions of the overhead line. The modern trolley bus may run short distances without the trolley lines. Recuperation of the kinetic energy at deceleration (braking) either into the overhead line network or by storing it in the vehicle is one of the characteristics of modern trolley buses

  1. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    Directory of Open Access Journals (Sweden)

    Penny David

    2007-10-01

    Full Text Available Abstract Background Alternative splicing has been reported in various eukaryotic groups including plants, apicomplexans, diatoms, amoebae, animals and fungi. However, whether widespread alternative splicing has evolved independently in the different eukaryotic groups or was inherited from their last common ancestor, and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional classes, cellular locations, intron/exon structures and evolutionary origins. Results For each species, we find that genes from most functional categories are alternatively spliced. Ancient genes (shared between animals, fungi and plants show high levels of alternative splicing. Genes with products expressed in the nucleus or plasma membrane are generally more alternatively spliced while those expressed in extracellular location show less alternative splicing. We find a clear correspondence between incidence of alternative splicing and intron number per gene both within and between genomes. In general, we find several similarities in patterns of alternative splicing across these diverse eukaryotes. Conclusion Along with previous studies indicating intron-rich genes with weak intron boundary consensus and complex spliceosomes in ancestral organisms, our results suggest that at least a simple form of alternative splicing may already have been present in the unicellular ancestor of plants, fungi and animals. A role for alternative splicing in the evolution of multicellularity then would largely have arisen by co-opting the preexisting process.

  2. Regulation of mammalian pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    HUI JingYi

    2009-01-01

    In eukaryotes, most protein-coding genes contain introns which are removed by precursor messenger RNA (pre-mRNA) splicing. Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct proteins. Recent genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene expression. Mis-regulation of splicing causes a wide range of human diseases. This review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA splicing. It also discusses emerging directions in the field of alternative splicing.

  3. Altered PLP1 splicing causes hypomyelination of early myelinating structures

    DEFF Research Database (Denmark)

    Kevelam, Sietske H; Taube, Jennifer R; van Spaendonk, Rosalina M L;

    2015-01-01

    causal mutations. In silico analysis of effects of the mutations on splicing and RNA folding was performed. In vitro gene splicing was examined in RNA from patients' fibroblasts and an immortalized immature oligodendrocyte cell line after transfection with mutant minigene splicing constructs. RESULTS......: All patients had unusual hemizygous mutations of PLP1 located in exon 3B (one deletion, one missense and two silent), which is spliced out in isoform DM20, or in intron 3 (five mutations). The deletion led to truncation of PLP1, but not DM20. Four mutations were predicted to affect PLP1/DM20...... alternative splicing by creating exonic splicing silencer motifs or new splice donor sites or by affecting the local RNA structure of the PLP1 splice donor site. Four deep intronic mutations were predicted to destabilize a long-distance interaction structure in the secondary PLP1 RNA fragment involved in...

  4. Regulation of mammalian pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    In eukaryotes,most protein-coding genes contain introns which are removed by precursor messenger RNA(pre-mRNA) splicing.Alternative splicing is a process by which multiple messenger RNAs(mRNAs) are generated from a single pre-mRNA,resulting in functionally distinct proteins.Recent genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene expression.Mis-regulation of splicing causes a wide range of human diseases.This review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA splicing.It also discusses emerging directions in the field of alternative splicing.

  5. Quality control of cadweld (mechanical) splices

    International Nuclear Information System (INIS)

    Test data for cadweld splicing of reinforcing steel collected during a study of quality assurance practices on nine nuclear power plant construction projects are presented and evaluated. These data lead to an important hypothesis that the visual inspection identifies procedural deficiencies, and the tensile test identifies material defects. It is also suggested that a material testing program and the visual inspection will detect essentially all substandard cadwell splices. This would permit the deletion of the expensive tensile testing program. Accordingly, most quality control programs require overtesting and overdocumentation of cadweld splices; and furthermore, these programs fail to recognize material defects. The project specifications and quality control requirements for the nine projects are compared. Where possible, these are evaluated against the industry standards and Federal regulations. It is shown that there are a number of deficiencies in these standards, and that in most cases, the testing requirements are not commensurate with the quality that is being achieved in the field

  6. Splicing variants of porcine synphilin-1

    DEFF Research Database (Denmark)

    Larsen, Knud Erik; Madsen, Lone Bruhn; Farajzadeh, Leila;

    2015-01-01

    (90%) and to mouse (84%) synphilin-1. Three shorter transcript variants of the synphilin-1 gene were identified, all lacking one or more exons. SNCAIP transcripts were detected in most examined organs and tissues and the highest expression was found in brain tissues and lung. Conserved splicing......RNA was investigated by RNAseq. The presented work reports the molecular cloning and characterization of the porcine (Sus scrofa) synphilin-1 cDNA (SNCAIP) and three splice variants hereof. The porcine SNCAIP cDNA codes for a protein (synphilin-1) of 919 amino acids which shows a high similarity to human...... variants and a novel splice form of synhilin-1 were found in this study. All synphilin-1 isoforms encoded by the identified transcript variants lack functional domains important for protein degradation....

  7. Rethinking passive transport: bus fare exemptions and young people's wellbeing.

    Science.gov (United States)

    Jones, Alasdair; Steinbach, Rebecca; Roberts, Helen; Goodman, Anna; Green, Judith

    2012-05-01

    Much recent public health research has emphasised the health impacts for young people of 'active travel' modes, typically defined as walking and cycling. Less research has focused on public transport modes. Drawing on qualitative data, we examine the links between bus travel and wellbeing in London, where young people currently have free bus travel. Our findings indicate that bus travel can be both a physically and socially active experience for young people. We suggest a more nuanced understanding of 'active travel' is now needed, alongside greater attention to urban public transport networks as key sites that impact on important determinants of wellbeing such as independent mobility and social inclusion. PMID:22321902

  8. Swisstrolley 3 - An articulated trolley bus; SWISSTROLLEY 3. Gelenktrolleybus

    Energy Technology Data Exchange (ETDEWEB)

    Gisler, H.-J.

    2006-07-01

    This illustrated final report for the Swiss Federal Office of Energy (SFOE) takes a look at the Swisstrolley 3 low-floor, articulated trolley bus developed in Switzerland by the Hess AG company. Various features of the variants of the trolley bus, including single vehicle, single and double-articulated buses and bus trailers, are discussed. The reductions achieved in energy consumption, weight and noise are discussed. Typical values for power consumption are presented in graphical form. Economic viability and passenger-friendliness are examined, as are possibilities for further improvement.

  9. Regulation of alternative splice site selection by reversible protein phosphorylation

    OpenAIRE

    Novoyatleva, Tatyana

    2007-01-01

    Splicing is the process that removes introns and joins exons from pre-mesenger RNA (pre-mRNA). It is an essential step in pre-mRNA processing that form the mature RNA. Microarray data indicates that approximately 75% of human genes produce transcripts that are alternatively spliced. Alternative splicing is one of the major mechanisms that ultimately generate high number of protein isoforms from a limited number of genes. The proper catalysis and regulation of alternative splice site selection...

  10. Progress toward therapy with antisense-mediated splicing modulation

    OpenAIRE

    Du, Liutao; Gatti, Richard A.

    2009-01-01

    Antisense oligonucleotides (AO) or antisense RNA can complementarily bind to a target site in pre-mRNA and regulate gene splicing, either to restore gene function by reprogramming gene splicing or to inhibit gene expression by disrupting splicing. These two applications represent novel therapeutic strategies for several types of diseases such as genetic disorders, cancers and infectious diseases. In this review, the recent developments and applications of antisense-mediated splicing modulatio...

  11. Cotranscriptional splicing efficiency differs dramatically between Drosophila and mouse

    OpenAIRE

    Khodor, Yevgenia L.; Menet, Jerome S; Tolan, Michael; Rosbash, Michael

    2012-01-01

    Spliceosome assembly and/or splicing of a nascent transcript may be crucial for proper isoform expression and gene regulation in higher eukaryotes. It has been shown that cotranscriptional splicing occurs efficiently in Drosophila, but there are not comparable genome-wide nascent splicing data from mammals. To provide this comparison, the authors analyzed a recently generated, high-throughput sequencing data set of mouse liver nascent RNA. Cotranscriptional splicing is approximately twofold l...

  12. Alternative splicing of DNA damage response genes and gastrointestinal cancers

    OpenAIRE

    Rahmutulla, Bahityar; Matsushita, Kazuyuki; Nomura, Fumio

    2014-01-01

    Alternative splicing, which is a common phenomenon in mammalian genomes, is a fundamental process of gene regulation and contributes to great protein diversity. Alternative splicing events not only occur in the normal gene regulation process but are also closely related to certain diseases including cancer. In this review, we briefly demonstrate the concept of alternative splicing and DNA damage and describe the association of alternative splicing and cancer pathogenesis, focusing on the pote...

  13. RNA structure and the mechanisms of alternative splicing

    OpenAIRE

    McManus, C. Joel; Graveley, Brenton R.

    2011-01-01

    Alternative splicing is a widespread means of increasing protein diversity and regulating gene expression in eukaryotes. Much progress has been made in understanding the proteins involved in regulating alternative splicing, the sequences they bind to, and how these interactions lead to changes in splicing patterns. However, several recent studies have identified other players involved in regulating alternative splicing. A major theme emerging from these studies is that RNA secondary structure...

  14. Evolution of alternative splicing in primate brain transcriptomes

    OpenAIRE

    Lin, Lan; Shen, Shihao; Jiang, Peng; Sato, Seiko; Davidson, Beverly L.; Xing, Yi

    2010-01-01

    Alternative splicing is a predominant form of gene regulation in higher eukaryotes. The evolution of alternative splicing provides an important mechanism for the acquisition of novel gene functions. In this work, we carried out a genome-wide phylogenetic survey of lineage-specific splicing patterns in the primate brain, via high-density exon junction array profiling of brain transcriptomes of humans, chimpanzees and rhesus macaques. We identified 509 genes showing splicing differences among t...

  15. SPA: a probabilistic algorithm for spliced alignment.

    Directory of Open Access Journals (Sweden)

    Erik van Nimwegen

    2006-04-01

    Full Text Available Recent large-scale cDNA sequencing efforts show that elaborate patterns of splice variation are responsible for much of the proteome diversity in higher eukaryotes. To obtain an accurate account of the repertoire of splice variants, and to gain insight into the mechanisms of alternative splicing, it is essential that cDNAs are very accurately mapped to their respective genomes. Currently available algorithms for cDNA-to-genome alignment do not reach the necessary level of accuracy because they use ad hoc scoring models that cannot correctly trade off the likelihoods of various sequencing errors against the probabilities of different gene structures. Here we develop a Bayesian probabilistic approach to cDNA-to-genome alignment. Gene structures are assigned prior probabilities based on the lengths of their introns and exons, and based on the sequences at their splice boundaries. A likelihood model for sequencing errors takes into account the rates at which misincorporation, as well as insertions and deletions of different lengths, occurs during sequencing. The parameters of both the prior and likelihood model can be automatically estimated from a set of cDNAs, thus enabling our method to adapt itself to different organisms and experimental procedures. We implemented our method in a fast cDNA-to-genome alignment program, SPA, and applied it to the FANTOM3 dataset of over 100,000 full-length mouse cDNAs and a dataset of over 20,000 full-length human cDNAs. Comparison with the results of four other mapping programs shows that SPA produces alignments of significantly higher quality. In particular, the quality of the SPA alignments near splice boundaries and SPA's mapping of the 5' and 3' ends of the cDNAs are highly improved, allowing for more accurate identification of transcript starts and ends, and accurate identification of subtle splice variations. Finally, our splice boundary analysis on the human dataset suggests the existence of a novel non

  16. SPA: A Probabilistic Algorithm for Spliced Alignment

    Science.gov (United States)

    van Nimwegen, Erik; Paul, Nicodeme; Sheridan, Robert; Zavolan, Mihaela

    2006-01-01

    Recent large-scale cDNA sequencing efforts show that elaborate patterns of splice variation are responsible for much of the proteome diversity in higher eukaryotes. To obtain an accurate account of the repertoire of splice variants, and to gain insight into the mechanisms of alternative splicing, it is essential that cDNAs are very accurately mapped to their respective genomes. Currently available algorithms for cDNA-to-genome alignment do not reach the necessary level of accuracy because they use ad hoc scoring models that cannot correctly trade off the likelihoods of various sequencing errors against the probabilities of different gene structures. Here we develop a Bayesian probabilistic approach to cDNA-to-genome alignment. Gene structures are assigned prior probabilities based on the lengths of their introns and exons, and based on the sequences at their splice boundaries. A likelihood model for sequencing errors takes into account the rates at which misincorporation, as well as insertions and deletions of different lengths, occurs during sequencing. The parameters of both the prior and likelihood model can be automatically estimated from a set of cDNAs, thus enabling our method to adapt itself to different organisms and experimental procedures. We implemented our method in a fast cDNA-to-genome alignment program, SPA, and applied it to the FANTOM3 dataset of over 100,000 full-length mouse cDNAs and a dataset of over 20,000 full-length human cDNAs. Comparison with the results of four other mapping programs shows that SPA produces alignments of significantly higher quality. In particular, the quality of the SPA alignments near splice boundaries and SPA's mapping of the 5′ and 3′ ends of the cDNAs are highly improved, allowing for more accurate identification of transcript starts and ends, and accurate identification of subtle splice variations. Finally, our splice boundary analysis on the human dataset suggests the existence of a novel non-canonical splice

  17. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Penny, David;

    2007-01-01

    , and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional...... classes, cellular locations, intron/exon structures and evolutionary origins. RESULTS: For each species, we find that genes from most functional categories are alternatively spliced. Ancient genes (shared between animals, fungi and plants) show high levels of alternative splicing. Genes with products...... expressed in the nucleus or plasma membrane are generally more alternatively spliced while those expressed in extracellular location show less alternative splicing. We find a clear correspondence between incidence of alternative splicing and intron number per gene both within and between genomes. In general...

  18. Implementation of viscoelastic Hopkinson bars

    Science.gov (United States)

    Curry, R.; Cloete, T.; Govender, R.

    2012-08-01

    Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic nature of the bar material. Implementing polymeric Hopkinson bars requires characterization of the viscoelastic properties of the material used. In this paper, 30 mm diameter Polymethyl Methacrylate bars are used as Hopkinson pressure bars. This testing technique is applied to polymeric foam called Divinycell H80 and H200. Although there is a large body of of literature containing compressive data, this rarely deals with strain rates above 250s-1 which becomes increasingly important when looking at the design of composite structures where energy absorption during impact events is high on the list of priorities. Testing of polymeric foams at high strain rates allows for the development of better constitutive models.

  19. Implementation of viscoelastic Hopkinson bars

    Directory of Open Access Journals (Sweden)

    Govender R.

    2012-08-01

    Full Text Available Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic nature of the bar material. Implementing polymeric Hopkinson bars requires characterization of the viscoelastic properties of the material used. In this paper, 30 mm diameter Polymethyl Methacrylate bars are used as Hopkinson pressure bars. This testing technique is applied to polymeric foam called Divinycell H80 and H200. Although there is a large body of of literature containing compressive data, this rarely deals with strain rates above 250s−1 which becomes increasingly important when looking at the design of composite structures where energy absorption during impact events is high on the list of priorities. Testing of polymeric foams at high strain rates allows for the development of better constitutive models.

  20. Dynamical Evolution of Barred Galaxies

    CERN Document Server

    Athanassoula, E

    2005-01-01

    Angular momentum redistribution within barred galaxies drives their dynamical evolution. Angular momentum is emitted mainly by near-resonant material in the bar region and absorbed by resonant material mainly in the outer disc and in the halo. This exchange determines the strength of the bar, the decrease of its pattern speed, as well as its morphology. If the galaxy has also a gaseous component and/or a companion or satellite, then these also take part in the angular momentum exchange. During the evolution a bar structure forms in the inner parts of the halo as well. This bar is shorter and fatter than the disc bar and stays so all through the simulation, although its length grows considerably with time. Viewed edge-on, the bar in the disc component acquires a boxy or peanut shape. I describe the families of periodic orbits that explain such structures and review the observations showing that boxy/peanut `bulges' are in fact just bars seen edge-on.

  1. SpliceMiner: a high-throughput database implementation of the NCBI Evidence Viewer for microarray splice variant analysis

    OpenAIRE

    Liu Hongfang; Ryan Michael C; Kahn Ari B; Zeeberg Barry R; Jamison D Curtis; Weinstein John N

    2007-01-01

    Abstract Background There are many fewer genes in the human genome than there are expressed transcripts. Alternative splicing is the reason. Alternatively spliced transcripts are often specific to tissue type, developmental stage, environmental condition, or disease state. Accurate analysis of microarray expression data and design of new arrays for alternative splicing require assessment of probes at the sequence and exon levels. Description SpliceMiner is a web interface for querying Evidenc...

  2. The epithelial splicing factors ESRP1 and ESRP2 positively and negatively regulate diverse types of alternative splicing events

    OpenAIRE

    Warzecha, Claude C.; Shen, Shihao; Xing, Yi; Carstens, Russ P.

    2009-01-01

    Cell-type and tissue-specific alternative splicing events are regulated by combinatorial control involving both abundant RNA binding proteins as well as those with more discrete expression and specialized functions. Epithelial Splicing Regulatory Proteins 1 and 2 (ESRP1 and ESRP2) are recently discovered epithelial-specific RNA binding proteins that promote splicing of the epithelial variant of the FGFR2, ENAH, CD44 and CTNND1 transcripts. To catalogue a larger set of splicing events under th...

  3. Niobium in engineering bar steels

    International Nuclear Information System (INIS)

    The use of niobium as a microalloying addition to hot rolled and cold finished bars offers many opportunities to achieve enhanced mechanical properties in a very cost effective manner. By the addition of niobium (sometimes in combination with vanadium) to selected carbon steel grades, it is possible to produce hot rolled bars with minimum yield strengths up to 689 MPa (100 ksi) and cold finished bars with yield strengths up to 1035 MPa (150 ksi). These high-strength-low-alloy bars have a myriad of applications particularly for automotive parts such as shafts, stabilizer bars, tie rods, piston rods, fasteners such as U-bolts, brackets, and various transmission parts. Of particular value has been the specification of Nb-microalloyed steels for such hot forgings as connecting rods and caps, and weld yokes a situation which has permitted elimination of post-forging heat treatment (quenching and tempering). Significant reductions in cost and energy consumption have been realized

  4. Bars in Cuspy Dark Halos

    CERN Document Server

    Dubinski, John; Shlosman, Isaac

    2008-01-01

    We examine the bar instability in models with an exponential disk and a cuspy NFW-like dark matter (DM) halo inspired by cosmological simulations. Bar evolution is studied as a function of numerical resolution in a sequence of models spanning 10K to 100M DM particles - including a multi-mass model with an effective resolution of 10G. The goal is to find convergence in dynamical behaviour. We characterize the bar growth, the buckling instability, pattern speed decay through resonant transfer of angular momentum, and possible destruction of the DM halo cusp. Overall, most characteristics converge in behaviour in detail for halos containing more than 10M particles. Notably, the formation of the bar does not destroy the density cusp in this case. These higher resolution simulations clearly illustrate the importance of discrete resonances in transporting angular momentum from the bar to the halo.

  5. Rfid-Based Automatic Bus Ticketing: Features and Trends

    Science.gov (United States)

    Oudah, A.

    2016-02-01

    Recent advancements in various technologies have made remarkable developments in various fields for public welfare and public transport is one such area. In the near future public bus transport system with advanced technologies like Radio Frequency Identification Device (RFID), GSM, GPS, ZigBee and RF modules will gain spotlight due to their advantage of higher convenience and greater life standards as compared to the conventional bus systems. In this paper, a comprehensive review of all several proposed bus ticketing and bus information methods has been presented in detail. The study brings out improved solution in terms of cost, convenience, user satisfaction and future implementation. The choice of working modules and their efficient performance has been discussed along with the highlighted importance of the need of technology for welfare of common public and visually impaired.

  6. Bus Passenger Recognition and Track of Video Sequence

    Directory of Open Access Journals (Sweden)

    Donghua Zhou

    2013-06-01

    Full Text Available Study on bus passenger recognition and track of video sequence is a research aiming at realizing making a statistics on passenger flow volume of bus, which not only helps traffic services center rationally schedule vehicles, but also can avoid overload. The system makes machine vision technology and digital image processing technique applied in customer counting of bus. The key questions in the study include effective collection of video image, effective extraction of moving object in sequence images, recognition of moving targets, trace and count of moving objects. The paper makes deep analysis on relevant technology from the perspective of the theory and practical application of the algorithm, and the study makes achievements. The theoretical algorithm for the study on bus passenger recognition and track of video sequence is distinctive. The practical experiments indicate that the system can satisfy the real-time requirements, and can accurately make statistics on the number of passengers, which has great application value.

  7. Optimization Model for Headway of a Suburban Bus Route

    Directory of Open Access Journals (Sweden)

    Xiaohong Jiang

    2014-01-01

    Full Text Available Due to relatively low passenger demand, headways of suburban bus route are usually longer than those of urban bus route. Actually it is also difficult to balance the benefits between passengers and operators, subject to the service standards from the government. Hence the headway of a suburban bus route is usually determined on the empirical experience of transport planners. To cope with this problem, this paper proposes an optimization model for designing the headways of suburban bus routes by minimizing the operating and user costs. The user costs take into account both the waiting time cost and the crowding cost. The feasibility and validity of the proposed model are shown by applying it to the Route 206 in Jiangning district, Nanjing city of China. Weightages of passengers’ cost and operating cost are further discussed, considering different passenger flows. It is found that the headway and objective function are affected by the weightages largely.

  8. Optimal control system of the intermittent bus-only approach

    Institute of Scientific and Technical Information of China (English)

    Jie Jiang; Yulin Chang

    2014-01-01

    To guarantee bus priority with a minimum impact on car traffic at intersections, an optimal control system of the intermittent bus-only approach (IBA) was proposed. The problems of the existing system are first solved through optimization: the judgment time of the IBA system was advanced to allow a bus to jump car queues if the bus was detected to arrive at the intersection, and the instant that the IBA lane became available to cars was controlled dynamically to increase the capacity of the IBA lane. The total car delay in one cycle was then analyzed quantitatively when implementing the optimal control system. The results show that in comparison with the existing system of the IBA, the car delay is greatly reduced and the probability of a car stopping twice is low after optimizing the IBA system.

  9. Galaxy Zoo: CANDELS Barred Disks and Bar Fractions

    CERN Document Server

    Simmons, B D; Lintott, Chris; Masters, Karen L; Willett, Kyle W; Keel, William C; Smethurst, R J; Cheung, Edmond; Nichol, Robert C; Schawinski, Kevin; Rutkowski, Michael; Kartaltepe, Jeyhan S; Bell, Eric F; Casteels, Kevin R V; Conselice, Christopher J; Almaini, Omar; Ferguson, Henry C; Fortson, Lucy; Hartley, William; Kocevski, Dale; Koekemoer, Anton M; McIntosh, Daniel H; Mortlock, Alice; Newman, Jeffrey A; Ownsworth, Jamie; Bamford, Steven; Dahlen, Tomas; Faber, Sandra M; Finkelstein, Steven L; Fontana, Adriano; Galametz, Audrey; Grogin, N A; Grutzbauch, Ruth; Guo, Yicheng; Haussler, Boris; Jek, Kian J; Kaviraj, Sugata; Lucas, Ray A; Peth, Michael; Salvato, Mara; Wiklind, Tommy; Wuyts, Stijn

    2014-01-01

    The formation of bars in disk galaxies is a tracer of the dynamical maturity of the population. Previous studies have found that the incidence of bars in disks decreases from the local Universe to z ~ 1, and by z > 1 simulations predict that bar features in dynamically mature disks should be extremely rare. Here we report the discovery of strong barred structures in massive disk galaxies at z ~ 1.5 in deep rest-frame optical images from CANDELS. From within a sample of 876 disk galaxies identified by visual classification in Galaxy Zoo, we identify 123 barred galaxies. Selecting a sub-sample within the same region of the evolving galaxy luminosity function (brighter than L*), we find that the bar fraction across the redshift range 0.5< z < 2 (f_bar = 10.7 +6.3 -3.5% after correcting for incompleteness) does not significantly evolve. We discuss the implications of this discovery in the context of existing simulations and our current understanding of the way disk galaxies have evolved over the last 11 bil...

  10. Auxiliary splice factor U2AF26 and transcription factor Gfi1 cooperate directly in regulating CD45 alternative splicing.

    NARCIS (Netherlands)

    Heyd, F.; Dam, G.B. ten; Moroy, T.

    2006-01-01

    By alternative splicing, different isoforms of the transmembrane tyrosine phosphatase CD45 are generated that either enhance or limit T cell receptor signaling. We report here that CD45 alternative splicing is regulated by cooperative action of the splice factor U2AF26 and the transcription factor G

  11. Common Traffic Violations of Bus Drivers in Urban China: An Observational Study

    OpenAIRE

    Qiqi Wang; , Wei Zhang; Rendong Yang; Yuanxiu Huang; Lin Zhang; Peishan Ning; Xunjie Cheng; Schwebel, David C; Guoqing Hu; Hongyan Yao

    2015-01-01

    Objective To report common traffic violations in bus drivers and the factors that influence those violations in urban China. Methods We conducted an observational study to record three types of traffic violations among bus drivers in Changsha City, China: illegal stopping at bus stations, violating traffic light signals, and distracted driving. The behaviors of bus drivers on 32 routes (20% of bus routes in the city) were observed. A two-level Poisson regression examined factors that predicte...

  12. Modelling traffic incidents to support dynamic bus fleets management for sustainable transport

    OpenAIRE

    Polyviou, Polyvios

    2011-01-01

    The continuous implementation of highly technological functions and specifically intelligent transport systems in public transport highlights the need of highly efficient, accurate and reliable bus operations network. Intelligent transport systems can support a variety of functions, including dynamic bus fleet management which has yet to be established in most bus fleets in the UK in a systematic way. In order to support dynamic bus fleet management by detecting the fundamental role of bus an...

  13. Bus designs for time-probabilistic multicore processors

    OpenAIRE

    Jalle Ibarra, Javier; Kosmidis, Leonidas; Abella Ferrer, Jaume; Quiñones Moreno, Eduardo; Cazorla Almeida, Francisco Javier

    2014-01-01

    Probabilistic Timing Analysis (PTA) reduces the amount of information needed to provide tight WCET estimates in real-time systems with respect to classic timing analysis. PTA imposes new requirements on hardware design that have been shown implementable for single-core architectures. However, no support has been proposed for multicores so far. In this paper, we propose several probabilistically-analysable bus designs for multicore processors ranging from 4 cores connected with a single bus, t...

  14. Bus Implementation Using New Low Power PFSCL Tristate Buffers

    OpenAIRE

    Neeta Pandey; Bharat Choudhary; Kirti Gupta; Ankit Mittal

    2016-01-01

    This paper proposes new positive feedback source coupled logic (PFSCL) tristate buffers suited to bus applications. The proposed buffers use switch to attain high impedance state and modify the load or the current source section. An interesting consequence of this is overall reduction in the power consumption. The proposed tristate buffers consume half the power compared to the available switch based counterpart. The issues with available PFSCL tristate buffers based bus implementation are id...

  15. A FPGA-based serial BUS test system design

    International Nuclear Information System (INIS)

    The serial BUS test system was introduced, it can use to analyze the errors of electrical and protocol. The test system is composed of two parts: electronics and software. The electronics include: impedance match electronics, signal conditioning electronics, AD convert electronics, FPGA DAQ electronics and USB interface. The software is divided into following blocks: firmware of FPGA and USB, USB driver, BUS protocol algorithm, operation and display interface. (authors)

  16. Capillary Electrophoresis Analysis of Conventional Splicing Assays

    DEFF Research Database (Denmark)

    de Garibay, Gorka Ruiz; Acedo, Alberto; García-Casado, Zaida;

    2014-01-01

    Rare sequence variants in "high-risk" disease genes, often referred as unclassified variants (UVs), pose a serious challenge to genetic testing. However, UVs resulting in splicing alterations can be readily assessed by in vitro assays. Unfortunately, analytical and clinical interpretation of thes...

  17. Alternative-splicing-mediated gene expression

    Science.gov (United States)

    Wang, Qianliang; Zhou, Tianshou

    2014-01-01

    Alternative splicing (AS) is a fundamental process during gene expression and has been found to be ubiquitous in eukaryotes. However, how AS impacts gene expression levels both quantitatively and qualitatively remains to be fully explored. Here, we analyze two common models of gene expression, each incorporating a simple splice mechanism that a pre-mRNA is spliced into two mature mRNA isoforms in a probabilistic manner. In the constitutive expression case, we show that the steady-state molecular numbers of two mature mRNA isoforms follow mutually independent Poisson distributions. In the bursting expression case, we demonstrate that the tail decay of the steady-state distribution for both mature mRNA isoforms that in general are not mutually independent can be characterized by the product of mean burst size and splicing probability. In both cases, we find that AS can efficiently modulate both the variability (measured by variance) and the noise level of the total mature mRNA, and in particular, the latter is always lower than the noise level of the pre-mRNA, implying that AS always reduces the noise. These results altogether reveal that AS is a mechanism of efficiently controlling the gene expression noise.

  18. Approaches to link RNA secondary structures with splicing regulation

    DEFF Research Database (Denmark)

    Plass, Mireya; Eyras, Eduardo

    2014-01-01

    facilitating or hindering the interaction with factors and small nuclear ribonucleoproteins (snRNPs) that regulate splicing. Moreover, the secondary structure could play a fundamental role in the splicing of yeast species, which lack many of the regulatory splicing factors present in metazoans. This chapter......In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by...

  19. CROSS-FRONTIER PUBLIC TRANSPORT: Y BUS

    CERN Multimedia

    CERN Housing Service

    2002-01-01

    'Y' buses cover the journey between Val Thoiry in France (shopping centre including'Migros' and other stores) and Blandonnet in Switzerland, where they stop outside the 'Jumbo' shop, now renamed 'Carrefour'. In France, the buses connect up Thoiry, Sergy and St. Genis, then cross the border to stop outside CERN, Meyrin, before continuing towards Geneva along the 'Route de Meyrin', stopping outside the 'La Tour' hospital, then at the terminus Blandonnet. The normal fare on French territory with a slight incursion into Switzerland for this purpose as far as CERN, Meyrin, is 1.20 Euros per single journey (ticket valid for one hour). The same amount is charged for any single journey in Switzerland. For example, the cost of a single journey between St. Genis and the 'La Tour' hospital would be 2.40 Euros. The season ticket ('Abonnement de Travail') opens the right to purchase a ticket valid over any period of seven days which entitles the holder to as many journeys as required between the bus stop nearest to the pl...

  20. Bus Routes, CTran Bus routes, Published in 2007, 1:1200 (1in=100ft) scale, Town of Cary NC.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as...

  1. Single Mode Fiber Optic Connectors And Splices

    Science.gov (United States)

    Woods, John G.

    1984-08-01

    There is a trend toward increasing use of single mode transmission, particularly in telecommunications where high data bit rates are transmitted for long distances. Inter-connections of multimode fibers can be made in a number of ways, using ferrules, v-grooves, elastomeric splices, etc. However, the connection of single mode fibers, which have core diameters of 4 to 13 μm, requires more precise alignment than do the multimode fibers having core diameters of 50 μm or more. At TRW, we have adapted the four rod alignment guide concept for single mode fiber inter-connections. The principle of this OPTAGUIDE* alignment guide is presented. The single mode connectors and splices use the four rod scheme with an index matching material to eliminate or reduce the losses incurred through fiber end roughness or angularity. We are able to produce demountable connectors for 80/4.4 pm fibers having typical insertion losses of 1.0dB. The main factors in obtaining this result are the naturally precise fiber alignment provided by the alignment guide, and the ability of several manufacturers to maintain tight diametral and core offset tolerances. The single mode OPTALIGN* SM Connectors have been subjected to performance and environmental tests including repeated matings, temperature cycle and vibration. The results of these tests are described in this paper. A feature of the OPTALIGN* SM Connectors is the relative ease and speed of attachment to fiber optic cable in the field, without the use of epoxy or polishing procedures. The alignment guide concept has also been applied to permanent single mode splices. The splicing procedure is simple to perform in the field without expensive or delicate equipment. Construction and assembly procedures of the demountable connectors and permanent splices will be described with the aid of diagrams and photographs.

  2. DIMETHYL ETHER (DME)-FUELED SHUTTLE BUS DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Elana M. Chapman; Shirish Bhide; Jennifer Stefanik; Howard Glunt; Andre L. Boehman; Allen Homan; David Klinikowski

    2003-04-01

    The objectives of this research and demonstration program are to convert a campus shuttle bus to operation on dimethyl ether, a potential ultra-clean alternative diesel fuel. To accomplish this objective, this project includes laboratory evaluation of a fuel conversion strategy, as well as, field demonstration of the DME-fueled shuttle bus. Since DME is a fuel with no lubricity (i.e., it does not possess the lubricating quality of diesel fuel), conventional fuel delivery and fuel injection systems are not compatible with dimethyl ether. Therefore, to operate a diesel engine on DME one must develop a fuel-tolerant injection system, or find a way to provide the necessary lubricity to the DME. In this project, they have chosen the latter strategy in order to achieve the objective with minimal need to modify the engine. Their strategy is to blend DME with diesel fuel, to obtain the necessary lubricity to protect the fuel injection system and to achieve low emissions. The bulk of the efforts over the past year were focused on the conversion of the campus shuttle bus. This process, started in August 2001, took until April 2002 to complete. The process culminated in an event to celebrate the launching of the shuttle bus on DME-diesel operation on April 19, 2002. The design of the system on the shuttle bus was patterned after the system developed in the engine laboratory, but also was subjected to a rigorous failure modes effects analysis (FMEA, referred to by Air Products as a ''HAZOP'' analysis) with help from Dr. James Hansel of Air Products. The result of this FMEA was the addition of layers of redundancy and over-pressure protection to the system on the shuttle bus. The system became operational in February 2002. Preliminary emissions tests and basic operation of the shuttle bus took place at the Pennsylvania Transportation Institute's test track facility near the University Park airport. After modification and optimization of the system on

  3. Blurring in bar code signals

    Science.gov (United States)

    Tang, Hong

    1997-10-01

    When a bar code symbol is passed over a scanner, it is struck across by a fast moving laser beam. The laser light is scattered by the bar code. The total scattered power is modulated by the reflectivity of the bars and spaces in the symbol. A fraction of the scattered light is collected and focused onto a photodetector that converts the light variation into an electronic signal. The electronic signal is then digitized for analysis by a computer. The scanning and detection process can be modeled by a convolution of the laser beam profile and the bar code reflectivity function. The switching between states in the digitized bar code signal, which represents transitions from a space to a bar or vice versa, is determined by a zero-crossing point in the second derivative of the analog signal. The laser profile acts like a smoothing function. It blurs the analog electronic signal. If the width of the laser profile is less than the minimum width of bars and spaces in the bar code reflectivity function, the transition point is not affected by the location of its neighboring edges. If the laser profile is wider than the minimum width in the bar code, the transition point can be shifted due to the locations of its neighboring edges. The behavior of the shift of transition is analyzed here for all cases in a UPC symbol. It is found that the amount of shift in the transition point is almost the same for several different cases within the depth of field of the scanner. The knowledge of the behavior of transition point shift can be used to accurately compensate printing errors in an over-printed bar code. The modulation transfer function (MTF) of bar code scanning is the Fourier transform of the marginal function of the scanning laser beam. The MTF through focus for a scanning system is presented. By using an aperture with central obscuration in the laser focusing system, the high frequency resolution of bar code scanning can be enhanced and the depth of field of the scanner can

  4. Valence band structure of the ZnO(1-bar 0-bar 1-bar -bar 0) surface studied by angle-resolved photoemission spectroscopy

    International Nuclear Information System (INIS)

    The electronic band structure of the ZnO(1-bar 0-bar 1-bar -bar 0) surface has been studied by angle-resolved photoemission spectroscopy utilizing synchrotron radiation. Photon-energy-dependent measurements and K- and O2-adsorption studies revealed that the O 2p dangling-bond state exists at 3.7eV below the Fermi level at the Γ-bar point. Polarization-dependent measurements show that the state has dominant contribution of the O 2px orbital (x is parallel to the (1-bar 2-bar -bar 1-bar 0) direction) at the Γ-bar point. It is found that the O 2p dangling-bond band lies within the projected bulk bands along the ΓX-bar -bar and-bar ΓX'-bar axes in the surface Brillouin zone. This result settles a controversial issue on the energetic position of the O 2p dangling-bond band, which has been in dispute among theoretical studies. The dispersion widths are found to be 0.8 and 0.5eV along the ΓX-bar -bar and-bar ΓX'-bar directions, respectively

  5. ANALISIS SARANA ANGKUTAN UMUM BUS DAMRI DI KOTA SEMARANG

    Directory of Open Access Journals (Sweden)

    Subhan Rizal Amin

    2015-12-01

    Full Text Available This study aims to analyze the availability and use of public transportation by taking a case study of the Damri bus which has Ngaliyan-Pucanggading route. The availability of public transport is not comparable to the demand for public transportation services so that the problems of transportation emerge. Additionally, the use of public transport becomes an important factor to develop the transportation. The study is conducted by surveying the Damri passengers of B.04 lane and logistic regression is used for the analysis. The method of determining the needs of transportation is issued by Departemen Perhubungan. It is used to calculate and determine whether the public transportation should be added or reduced. Then, the factors used as the independent variables in the analysis are the Damri’s safety, effectiveness, efficiency, service and reliability. The results show that Perum Damri does not need to add more Damri bus for B.04 because the results’ calculation is 0.6. and the factors that significantly influence the use Damri bus is the variable of safety, effectiveness and efficiency. However, the variables of service and reliability do not significantly influence the use of B.04 Damri bus.Penelitian ini bertujuan untuk menganalisis ketersediaan dan penggunaan angkutan sarana angkutan umum penumpang dengan mengambil studi kasus pada bus Damri jalur dengan trayek Ngaliyan-Pucanggading. Ketersediaan sarana angkutan umum yang tersedia tidak sebanding dengan besarnya permintaan akan jasa angkutan umum oleh masyarakat umum sehingga muncul permasalahan transportasi. Di samping itu, penggunaan angkutan umum yang beroperasi menjadi faktor penting dalam upaya pengembangan sarana transportasi. Penelitian ini dilakukan dengan menggunakan metode survey terhadap para penumpang bus Damri jalur B.04 dan dianalisis dengan Regresi Logistik. Metode penentuan kebutuhan armada dikeluarkan oleh Departemen Perhubungan yang digunakan untuk menghitung apakah suatu

  6. Quasinuclear N bar N states

    International Nuclear Information System (INIS)

    We present an interpretation of recent experimental results on nucleon-anti-nucleon annihilation reactions of the type N bar N → πX, which have yielded evidence for a new tensor meson AX (here called X2) with JπC(IG) = 2++(0+). The branching ratios for producing X2 from N bar N atomic states of orbital angular momentum L = 0,1, as well as its preference for decay into ρρ and ππ rather than K bar K channels, are consistent with the identifications of X2 as a 13P2-13F2 bound state of the N bar N potential. We suggest further key test of this interpretation. 21 refs

  7. SpliceMiner: a high-throughput database implementation of the NCBI Evidence Viewer for microarray splice variant analysis

    Directory of Open Access Journals (Sweden)

    Liu Hongfang

    2007-03-01

    Full Text Available Abstract Background There are many fewer genes in the human genome than there are expressed transcripts. Alternative splicing is the reason. Alternatively spliced transcripts are often specific to tissue type, developmental stage, environmental condition, or disease state. Accurate analysis of microarray expression data and design of new arrays for alternative splicing require assessment of probes at the sequence and exon levels. Description SpliceMiner is a web interface for querying Evidence Viewer Database (EVDB. EVDB is a comprehensive, non-redundant compendium of splice variant data for human genes. We constructed EVDB as a queryable implementation of the NCBI Evidence Viewer (EV. EVDB is based on data obtained from NCBI Entrez Gene and EV. The automated EVDB build process uses only complete coding sequences, which may or may not include partial or complete 5' and 3' UTRs, and filters redundant splice variants. Unlike EV, which supports only one-at-a-time queries, SpliceMiner supports high-throughput batch queries and provides results in an easily parsable format. SpliceMiner maps probes to splice variants, effectively delineating the variants identified by a probe. Conclusion EVDB can be queried by gene symbol, genomic coordinates, or probe sequence via a user-friendly web-based tool we call SpliceMiner (http://discover.nci.nih.gov/spliceminer. The EVDB/SpliceMiner combination provides an interface with human splice variant information and, going beyond the very valuable NCBI Evidence Viewer, supports fluent, high-throughput analysis. Integration of EVDB information into microarray analysis and design pipelines has the potential to improve the analysis and bioinformatic interpretation of gene expression data, for both batch and interactive processing. For example, whenever a gene expression value is recognized as important or appears anomalous in a microarray experiment, the interactive mode of SpliceMiner can be used quickly and easily to

  8. Electromagnetic Compressive Split Hopkinson Bar

    OpenAIRE

    Martins, P. A. F.; Rosa, P. A. R.; Silva, C. M. A.

    2008-01-01

    This paper proposes a new design for the compressive split Hopkinson bar that makes use of the intense pressure created in a transient magnetic field formed by the passage of a pulse of electric current through a series of coils. The proposed technology enables to characterize the behaviour of materials under high strain-rates with a small acceleration path length of the striker bar and, because propulsion is purely electromagnetic, the overall performance can be easily controlled and nearly ...

  9. Strength Degradation of Gfrp Bars

    OpenAIRE

    Bhise, Vikrant Sudhakar

    2002-01-01

    The primary objective of this research was to examine the strength degradation of Glass Fiber Reinforced Polymer (GFRP) bars at high temperature and alkalinity and determine if an Arrhenius type relationship can be used as a means of projecting life. The work done includes a thorough literature review, experiments and development of strength prediction models. The experimental work involves exposure of GFRP bars incased in cement mortar to lime-water solution at 30, 45 and 57°C. Overall 100 ...

  10. Multiprobe quantum spin Hall bars

    OpenAIRE

    Sanvito, Stefano

    2013-01-01

    PUBLISHED We analyze electron transport in multiprobe quantum spin Hall (QSH) bars using the B¨uttiker formalism and draw parallels with their quantum Hall (QH) counterparts. We find that in a QSH bar the measured resistance changes upon introducing side voltage probes, in contrast to the QH case. We also study four- and six-terminal geometries and derive the expressions for the resistances. For these our analysis is generalized from the single-channel to the multi-channel case...

  11. Barred disks in dense environments

    CERN Document Server

    Marinova, I; Heiderman, A; Barazza, F D; Gray, M E; Barden, M; Wolf, C; Peng, C Y; Bacon, D; Balogh, M; Bell, E F; Bohm, A; Caldwell, J A R; Haussler, B; Heymans, C; Jahnke, K; van Kampen, E; Koposov, S; Lane, K; McIntosh, D H; Meisenheimer, K; Rix, H -W; Sanchez, S F; Taylor, A; Wisotzki, L; Zheng, X

    2010-01-01

    We investigate the properties of bright (MV <= -18) barred and unbarred disks in the Abell 901/902 cluster system at z~0.165 with the STAGES HST ACS survey. To identify and characterize bars, we use ellipse-fitting. We use visual classification, a Sersic cut, and a color cut to select disk galaxies, and find that the latter two methods miss 31% and 51%, respectively of disk galaxies identified through visual classification. This underscores the importance of carefully selecting the disk sample in cluster environments. However, we find that the global optical bar fraction in the clusters is ~30% regardless of the method of disk selection. We study the relationship of the optical bar fraction to host galaxy properties, and find that the optical bar fraction depends strongly on the luminosity of the galaxy and whether it hosts a prominent bulge or is bulgeless. Within a given absolute magnitude bin, the optical bar fraction increases for galaxies with no significant bulge component. Within each morphological ...

  12. Bar Formation from Galaxy Flybys

    CERN Document Server

    Lang, Meagan; Sinha, Manodeep

    2014-01-01

    Recently, both simulations and observations have revealed that flybys - fast, one-time interactions between two galaxy halos - are surprisingly common, nearing/comparable to galaxy mergers. Since these are rapid, transient events with the closest approach well outside the galaxy disk, it is unclear if flybys can transform the galaxy in a lasting way. We conduct collisionless N-body simulations of three co-planer flyby interactions between pure-disk galaxies to take a first look at the effects flybys have on disk structure, with particular focus on stellar bar formation. We find that some flybys are capable of inciting a bar with bars forming in both galaxies during our 1:1 interaction and in the secondary during our 10:1 interaction. The bars formed have ellipticities >0.5, sizes on the order of the host disk's scale length, and persist to the end of our simulations, ~5 Gyr after pericenter. The ability of flybys to incite bar formation implies that many processes associated with secular bar evolution may be ...

  13. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates.

    Directory of Open Access Journals (Sweden)

    Manuel Irimia

    Full Text Available Alternative splicing is tightly regulated in a spatio-temporal and quantitative manner. This regulation is achieved by a complex interplay between spliceosomal (trans factors that bind to different sequence (cis elements. cis-elements reside in both introns and exons and may either enhance or silence splicing. Differential combinations of cis-elements allows for a huge diversity of overall splicing signals, together comprising a complex 'splicing code'. Many cis-elements have been identified, and their effects on exon inclusion levels demonstrated in reporter systems. However, the impact of interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs on exon inclusion levels in human and chimpanzee. For this purpose, we compiled and studied comprehensive datasets of predicted ESRs, identified by several computational and experimental approaches, as well as microarray data for changes in alternative splicing levels between human and chimpanzee. Surprisingly, we found no association between changes in predicted ESRs and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little or no effect on splicing, and thus interspecific changes at short-time scales may primarily occur in these effectively neutral ESRs. These results underscore the difficulties of using current computational ESR prediction algorithms to identify truly functionally important motifs, and provide a cautionary tale for studies of the effect of SNPs on splicing in human disease.

  14. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates.

    Science.gov (United States)

    Irimia, Manuel; Rukov, Jakob Lewin; Roy, Scott William

    2009-01-01

    Alternative splicing is tightly regulated in a spatio-temporal and quantitative manner. This regulation is achieved by a complex interplay between spliceosomal (trans) factors that bind to different sequence (cis) elements. cis-elements reside in both introns and exons and may either enhance or silence splicing. Differential combinations of cis-elements allows for a huge diversity of overall splicing signals, together comprising a complex 'splicing code'. Many cis-elements have been identified, and their effects on exon inclusion levels demonstrated in reporter systems. However, the impact of interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs) on exon inclusion levels in human and chimpanzee. For this purpose, we compiled and studied comprehensive datasets of predicted ESRs, identified by several computational and experimental approaches, as well as microarray data for changes in alternative splicing levels between human and chimpanzee. Surprisingly, we found no association between changes in predicted ESRs and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little or no effect on splicing, and thus interspecific changes at short-time scales may primarily occur in these effectively neutral ESRs. These results underscore the difficulties of using current computational ESR prediction algorithms to identify truly functionally important motifs, and provide a cautionary tale for studies of the effect of SNPs on splicing in human disease. PMID:19495418

  15. Entropic contributions to the splicing process

    International Nuclear Information System (INIS)

    It has been recently argued that depletion attraction may play an important role in different aspects of cellular organization, ranging from the organization of transcriptional activity in transcription factories to the formation of nuclear bodies. In this paper, we suggest a new application of these ideas in the context of the splicing process, a crucial step of messenger RNA maturation in eukaryotes. We shall show that entropy effects and the resulting depletion attraction may explain the relevance of the aspecific intron length variable in the choice of splice-site recognition modality. On top of that, some qualitative features of the genome architecture of higher eukaryotes can find evolutionary realistic motivation in the light of our model

  16. Car Delay Model near Bus Stops with Mixed Traffic Flow

    Directory of Open Access Journals (Sweden)

    Yang Xiaobao

    2013-01-01

    Full Text Available This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the stopping buses. The second occurs as buses merge back to the motorized lane. The average car delay is estimated as the sum of the average delay at these two conflict points and the delay resulting from following the slower bicycles that merged into the motorized lane. Data are collected to calibrate and validate the developed model from one site in Beijing. The sensitivity of car delay to various operation conditions is examined. The results show that both bus stream and bicycle stream have significant effects on car delay. At bus volumes above 200 vehicles per hour, the curbside stop design is not appropriate because of the long car delays. It can be replaced by the bus bay design.

  17. Splicing therapy for neuromuscular disease ☆

    OpenAIRE

    Andrew G. L. Douglas; Wood, Matthew J. A.

    2013-01-01

    Duchenne muscular dystrophy (DMD) and spinal muscular atrophy (SMA) are two of the most common inherited neuromuscular diseases in humans. Both conditions are fatal and no clinically available treatments are able to significantly alter disease course in either case. However, by manipulation of pre-mRNA splicing using antisense oligonucleotides, defective transcripts from the DMD gene and from the SMN2 gene in SMA can be modified to once again produce protein and restore function. A large numb...

  18. Resolving deconvolution ambiguity in gene alternative splicing

    Directory of Open Access Journals (Sweden)

    Hubbell Earl

    2009-08-01

    Full Text Available Abstract Background For many gene structures it is impossible to resolve intensity data uniquely to establish abundances of splice variants. This was empirically noted by Wang et al. in which it was called a "degeneracy problem". The ambiguity results from an ill-posed problem where additional information is needed in order to obtain an unique answer in splice variant deconvolution. Results In this paper, we analyze the situations under which the problem occurs and perform a rigorous mathematical study which gives necessary and sufficient conditions on how many and what type of constraints are needed to resolve all ambiguity. This analysis is generally applicable to matrix models of splice variants. We explore the proposal that probe sequence information may provide sufficient additional constraints to resolve real-world instances. However, probe behavior cannot be predicted with sufficient accuracy by any existing probe sequence model, and so we present a Bayesian framework for estimating variant abundances by incorporating the prediction uncertainty from the micro-model of probe responsiveness into the macro-model of probe intensities. Conclusion The matrix analysis of constraints provides a tool for detecting real-world instances in which additional constraints may be necessary to resolve splice variants. While purely mathematical constraints can be stated without error, real-world constraints may themselves be poorly resolved. Our Bayesian framework provides a generic solution to the problem of uniquely estimating transcript abundances given additional constraints that themselves may be uncertain, such as regression fit to probe sequence models. We demonstrate the efficacy of it by extensive simulations as well as various biological data.

  19. Alternative Splicing Programs in Prostate Cancer

    OpenAIRE

    Claudio Sette

    2013-01-01

    Prostate cancer (PCa) remains one of the most frequent causes of death for cancer in the male population. Although the initial antiandrogenic therapies are efficacious, PCa often evolves into a hormone-resistant, incurable disease. The genetic and phenotypic heterogeneity of this type of cancer renders its diagnosis and cure particularly challenging. Mounting evidence indicates that alternative splicing, the process that allows production of multiple mRNA variants from each gene, contributes ...

  20. A note on the B*B-bar, B*B-bar*, D*D-bar, D*D-bar* molecular states

    International Nuclear Information System (INIS)

    In the framework of the one-boson-exchange model, we have performed an extensive study of the possible B*B-bar, B*B-bar*, D*D-bar, D*D-bar* molecular states with various quantum numbers after considering the S-wave and D-wave mixing. We also discuss the possible experimental research of these interesting states. (authors)

  1. Splicing variants of porcine synphilin-1

    Directory of Open Access Journals (Sweden)

    Knud Larsen

    2015-09-01

    Full Text Available Parkinson's disease (PD, idiopathic and familial, is characterized by degradation of dopaminergic neurons and the presence of Lewy bodies (LB in the substantia nigra. LBs contain aggregated proteins of which α-synuclein is the major component. The protein synphilin-1 interacts and colocalizes with α-synuclein in LBs. The aim of this study was to isolate and characterize porcine synphilin-1 and isoforms hereof with the future perspective to use the pig as a model for Parkinson's disease. The porcine SNCAIP cDNA was cloned by reverse transcriptase PCR. The spatial expression of SNCAIP mRNA was investigated by RNAseq. The presented work reports the molecular cloning and characterization of the porcine (Sus scrofa synphilin-1 cDNA (SNCAIP and three splice variants hereof. The porcine SNCAIP cDNA codes for a protein (synphilin-1 of 919 amino acids which shows a high similarity to human (90% and to mouse (84% synphilin-1. Three shorter transcript variants of the synphilin-1 gene were identified, all lacking one or more exons. SNCAIP transcripts were detected in most examined organs and tissues and the highest expression was found in brain tissues and lung. Conserved splicing variants and a novel splice form of synhilin-1 were found in this study. All synphilin-1 isoforms encoded by the identified transcript variants lack functional domains important for protein degradation.

  2. A new method for splice site prediction based on the sequence patterns of splicing signals and regulatory elements

    Institute of Scientific and Technical Information of China (English)

    SUN ZongXiao; SANG LingJie; JU LiNing; ZHU HuaiQiu

    2008-01-01

    It is of significance for splice site prediction to develop novel algorithms that combine the sequence patterns of regulatory elements such as enhancers and silencers with the patterns of splicing signals. In this paper, a statistical model of splicing signals was built based on the entropy density profile (EDP) method, weight array method (WAM) and κ test; moreover, the model of splicing regulatory elements was developed by an unsupervised self-learning method to detect motifs associated with regulatory elements. With two models incorporated, a multi-level support vector machine (SVM) system was de-vised to perform ab initio prediction for splice sites originating from DNA sequence in eukaryotic ge-home. Results of large scale tests on human genomic splice sites show that the new method achieves a comparative high performance in splice site prediction. The method is demonstrated to be with at least the same level of performance and usually better performance than the existing SpliceScan method based on modeling regulatory elements, and shown to have higher accuracies than the traditional methods with modeling splicing signals such as the GeneSplicer. In particular, the method has evident advantage over splice site prediction for the genes with lower GC content.

  3. A study of alternative splicing in the pig

    Directory of Open Access Journals (Sweden)

    Jørgensen Claus B

    2010-05-01

    Full Text Available Abstract Background Since at least half of the genes in mammalian genomes are subjected to alternative splicing, alternative pre-mRNA splicing plays an important contribution to the complexity of the mammalian proteome. Expressed sequence tags (ESTs provide evidence of a great number of possible alternative isoforms. With the EST resource for the domestic pig now containing more than one million porcine ESTs, it is possible to identify alternative splice forms of the individual transcripts in this species from the EST data with some confidence. Results The pig EST data generated by the Sino-Danish Pig Genome project has been assembled with publicly available ESTs and made available in the PigEST database. Using the Distiller package 2,515 EST clusters with candidate alternative isoforms were identified in the EST data with high confidence. In agreement with general observations in human and mouse, we find putative splice variants in about 30% of the contigs with more than 50 ESTs. Based on the criteria that a minimum of two EST sequences confirmed each splice event, a list of 100 genes with the most distinct tissue-specific alternative splice events was generated from the list of candidates. To confirm the tissue specificity of the splice events, 10 genes with functional annotation were randomly selected from which 16 individual splice events were chosen for experimental verification by quantitative PCR (qPCR. Six genes were shown to have tissue specific alternatively spliced transcripts with expression patterns matching those of the EST data. The remaining four genes had tissue-restricted expression of alternative spliced transcripts. Five out of the 16 splice events that were experimentally verified were found to be putative pig specific. Conclusions In accordance with human and rodent studies we estimate that approximately 30% of the porcine genes undergo alternative splicing. We found a good correlation between EST predicted tissue

  4. Modulation transfer function of bar code scanning

    Science.gov (United States)

    Tang, Hong; Milster, Tom D.

    1998-09-01

    Bar code scanners are ubiquitous in supermarkets. As a bar code is passed over a scanner, a laser beam scans across the bar code. The scattered light is modulated by the reflectivity of the bars and spaces of the bar code. The bar code scanning process can be described as a 1D convolution of the scanning laser profile and the bar code reflectivity function. The modulation transfer function (MTF) of bar code scanning is the Fourier transform of the marginal profile of the laser beam. The properties of the MTF of bar code scanning is similar to that of an incoherent imaging system. Measurements of the MTF of bar code scanning at one focus position are presented. The experimental results are then discussed.

  5. Splicing modulation therapy in the treatment of genetic diseases

    Directory of Open Access Journals (Sweden)

    Arechavala-Gomeza V

    2014-12-01

    Full Text Available Virginia Arechavala-Gomeza,1 Bernard Khoo,2 Annemieke Aartsma-Rus3 1Neuromuscular Disorders Group, BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain; 2Endocrinology, Division of Medicine, University College London, London, UK; 3Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands All authors contributed equally to this manuscript Abstract: Antisense-mediated splicing modulation is a tool that can be exploited in several ways to provide a potential therapy for rare genetic diseases. This approach is currently being tested in clinical trials for Duchenne muscular dystrophy and spinal muscular atrophy. The present review outlines the versatility of the approach to correct cryptic splicing, modulate alternative splicing, restore the open reading frame, and induce protein knockdown, providing examples of each. Finally, we outline a possible path forward toward the clinical application of this approach for a wide variety of inherited rare diseases. Keywords: splicing, therapy, antisense oligonucleotides, cryptic splicing, alternative splicing

  6. Accumulation of GC donor splice signals in mammals

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2008-07-01

    Full Text Available Abstract The GT dinucleotide in the first two intron positions is the most conserved element of the U2 donor splice signals. However, in a small fraction of donor sites, GT is replaced by GC. A substantial enrichment of GC in donor sites of alternatively spliced genes has been observed previously in human, nematode and Arabidopsis, suggesting that GC signals are important for regulation of alternative splicing. We used parsimony analysis to reconstruct evolution of donor splice sites and inferred 298 GT > GC conversion events compared to 40 GC > GT conversion events in primate and rodent genomes. Thus, there was substantive accumulation of GC donor splice sites during the evolution of mammals. Accumulation of GC sites might have been driven by selection for alternative splicing. Reviewers This article was reviewed by Jerzy Jurka and Anton Nekrutenko. For the full reviews, please go to the Reviewers' Reports section.

  7. The implications of alternative splicing in the ENCODE protein complement

    DEFF Research Database (Denmark)

    Tress, Michael L.; Martelli, Pier Luigi; Frankish, Adam;

    2007-01-01

    Alternative premessenger RNA splicing enables genes to generate more than one gene product. Splicing events that occur within protein coding regions have the potential to alter the biological function of the expressed protein and even to create new protein functions. Alternative splicing has been...... suggested as one explanation for the discrepancy between the number of human genes and functional complexity. Here, we carry out a detailed study of the alternatively spliced gene products annotated in the ENCODE pilot project. We find that alternative splicing in human genes is more frequent than has...... commonly been suggested, and we demonstrate that many of the potential alternative gene products will have markedly different structure and function from their constitutively spliced counterparts. For the vast majority of these alternative isoforms, little evidence exists to suggest they have a role as...

  8. Gas flow in barred potentials II. Bar Driven Spiral Arms

    CERN Document Server

    Sormani, Mattia C; Magorrian, John

    2015-01-01

    Spiral arms that emerge from the ends of a galactic bar are important in interpreting observations of our and external galaxies. It is therefore important to understand the physical mechanism that causes them. We find that these spiral arms can be understood as kinematic density waves generated by librations around underlying ballistic closed orbits. This is even true in the case of a strong bar, provided the librations are around the appropriate closed orbits and not around the circular orbits that form the basis of the epicycle approximation. An important consequence is that it is a potential's orbital structure that determines whether a bar should be classified as weak or strong, and not crude estimates of the potential's deviation from axisymmetry.

  9. High Performance BDR for the PCU of AlphaBus

    Science.gov (United States)

    Subrier, Laurent; Besdel, Patrick; Daubresse, Thierry; Trehet, Eric

    2008-09-01

    Very high-power satellites for Telecom missions require 100V bus. Some Telecom payloads may be used in TDMA mode with a power modulation up to 30% of the Payload power. Full performance of the PCU is required in this case even in eclipse mode. It is clear that this requirement is very demanding especially for the BDR design.100V bus system leads making this requirement more difficult as Bus and Battery short-circuit protections have to operate under a voltage of 100V. This paper describes first the overall BDR design with a special focus on the innovative battery short-circuit protection that is not achieved by a "classical" series SSPC on the battery line.The constraints brought by the TDMA mode are detailed, especially on the input filter of the BDR in presence of large battery inductance. The solution developed to cope with large TDMA current is then presented with the associated TDMA performance.

  10. Contagion processes on urban bus networks in Indian cities

    CERN Document Server

    Chatterjee, Atanu; Jagannathan, Krishna

    2015-01-01

    Bus transportation is considered as one of the most convenient and cheapest modes of public transportation in Indian cities. Due to their cost-effectiveness and wide reachability, they help a significant portion of the human population in cities to reach their destinations every day. Although from a transportation point of view they have numerous advantages over other modes of public transportation, they also pose a serious threat of contagious diseases spreading throughout the city. The presence of numerous local spatial constraints makes the process and extent of epidemic spreading extremely difficult to predict. Also, majority of the studies have focused on the contagion processes on scale-free network topologies whereas, spatially-constrained real-world networks such as, bus networks exhibit a wide-spectrum of network topology. Therefore, we aim in this study to understand this complex dynamical process of epidemic outbreak and information diffusion on the bus networks for six different Indian cities usin...

  11. American Fuel Cell Bus Project Evaluation. Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-09-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB was developed as part of the Federal Transit Administration's (FTA's) National Fuel Cell Bus Program. Through the non-profit consortia CALSTART, a team led by SunLine Transit Agency and BAE Systems developed a new fuel cell electric bus for demonstration. SunLine added two more AFCBs to its fleet in 2014 and another in 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report summarizes the performance results for the buses through June 2015.

  12. Tissue-specific splicing factor gene expression signatures

    OpenAIRE

    Grosso, A. R.; Gomes, Anita; Barbosa-Morais, Nuno; Caldeira, Sandra; Thorne, Natalie; Grech, Godfrey; Lindern, Marieke; Carmo-Fonseca, Maria

    2008-01-01

    textabstractThe alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that tissue-specific splicing decisions most likely result from differences in the concentration and/or activity of these proteins. However, large-scale data to systematically address this issue have just recently...

  13. Alternative Splicing and Its Impact as a Cancer Diagnostic Marker

    OpenAIRE

    Kim, Yun-Ji; Kim, Heui-Soo

    2012-01-01

    Most genes are processed by alternative splicing for gene expression, resulting in the complexity of the transcriptome in eukaryotes. It allows a limited number of genes to encode various proteins with intricate functions. Alternative splicing is regulated by genetic mutations in cis-regulatory factors and epigenetic events. Furthermore, splicing events occur differently according to cell type, developmental stage, and various diseases, including cancer. Genome instability and flexible proteo...

  14. Pre-mRNA splicing in disease and therapeutics

    OpenAIRE

    Singh, Ravi K.; Cooper, Thomas A.

    2012-01-01

    In metazoans, alternative splicing of genes is essential for regulating gene expression and contributing to functional complexity. Computational predictions, comparative genomics, and transcriptome profiling of normal and diseased tissues indicate an unexpectedly high fraction of diseases are caused by mutations that alter splicing. Mutations in cis elements cause mis-splicing of genes that alter gene function and contribute to disease pathology. Mutations of core spliceosomal factors are ass...

  15. Phosphorylation-Mediated Regulation of Alternative Splicing in Cancer

    OpenAIRE

    Chiara Naro; Claudio Sette

    2013-01-01

    Alternative splicing (AS) is one of the key processes involved in the regulation of gene expression in eukaryotic cells. AS catalyzes the removal of intronic sequences and the joining of selected exons, thus ensuring the correct processing of the primary transcript into the mature mRNA. The combinatorial nature of AS allows a great expansion of the genome coding potential, as multiple splice-variants encoding for different proteins may arise from a single gene. Splicing is mediated by a large...

  16. Embracing the complexity of pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    Peter J Shepard; Klemens J Hertel

    2010-01-01

    @@ Pre-mRNA splicing is a fundamental process required for the expression of most metazoan genes. It is carried out by the spliceosome, which catalyzes the removal of non-coding intronic sequences to assemble exons into mature mRNAs prior to export and translation.Defects in splicing lead to many human genetic diseases [1], and splicing mutations in a number of genes involved in growth control have been implicated in multiple types of cancer.

  17. Study of fast transients generated in a GIS bus duct in compressed $SF_6-N_2$ gas mixtures containing lower percentages of $SF_6$

    OpenAIRE

    Singha, S.; Joy, Thomas M; Naidu, MS

    2000-01-01

    This paper deals with the study of Very Fast Transient Overvoltages (VFTO) with $SF_6-N_2$ gas mixture as the insulating medium, but with lower percentages of $SF_6$ in the mixture. The investigations are performed using a laboratory model bus duct. A capacitive voltage sensor was used to measure the peak VFTO and temporal characteristics. Measurements were carried out in $SF_6-N_2$ gas mixtures wherein the concentration of $SF_6$ was varied from 0 to 20% over a pressure range of 1 to 5 bars....

  18. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    , and regenerative braking for battery charging. It uses a 19.3 kW Ballard PEM fuel cell, will store 12.6 kg of hydrogen at 350 Bar, and includes a 60 kWh battery storage system. The objectives of the project included the following: (a) To advance commercialization of hydrogen-powered transit buses and supporting infrastructure; (b) To provide public outreach and education by showcasing the operation of a 22-foot fuel cell hybrid shuttle bus and Texas first hydrogen fueling infrastructure; and (c) To showcase operation of zero-emissions vehicle for potential transit applications. As mentioned above, the project successfully demonstrated an early vehicle technology, the Ebus plug-in hybrid fuel cell bus, and that success has led to the acquisition of a more advanced vehicle that can take advantage of the same fueling infrastructure. Needed hydrogen station improvements have been identified that will enhance the capabilities of the fueling infrastructure to serve the new bus and to meet the transit agency needs. Over the course of this project, public officials, local government staff, and transit operators were engaged in outreach and education activities that acquainted them with the real world operation of a fuel cell bus and fueling infrastructure. Transit staff members in the Dallas/Ft. Worth region were invited to a workshop in Arlington, Texas at the North Central Texas Council of Governments to participate in a workshop on hydrogen and fuel cells, and to see the fuel cell bus in operation. The bus was trucked to the meeting for this purpose so that participants could see and ride the bus. Austin area transit staff members visited the fueling site in Austin to be briefed on the bus and to participate in a fueling demonstration. This led to further meetings to determine how a fuel cell bus and fueling station could be deployed at Capital Metro Transit. Target urban regions that expressed additional interest during the project in response to the outreach meetings and

  19. On-chip bus standards in a broadcast architecture

    OpenAIRE

    Romaguera i Restudis, Josep-Oriol

    2012-01-01

    [ANGLÈS] This final project has been developed in the School of Electrical Engineering (in Aalto University) and its main goal is to value whether AMBA (Advanced Microcontroller Bus Architecture) is a good option to develop an on-chip broadcast architecture. To carry it out, various alternatives are taken in account: use full AMBA (with all its signals), use CAN (Controller Area Network) bus instead of AMBA, and use modified AMBA (using only the signals that will be used). Once one alternativ...

  20. Fuzzy logic control for active bus suspension system

    International Nuclear Information System (INIS)

    In this study an active controller is presented for vibration suppression of a full-bus suspension model that use air spring. Since the air spring on the full-bus model may face different working conditions, auxiliary chambers have been designed. The vibrations, caused by the irregularities of the road surfaces, are tried to be suppressed via a multi input-single output fuzzy logic controller. The effect of changes in the number of auxiliary chambers on the vehicle vibrations is also investigated. The numerical results demonstrate that the presented fuzzy logic controller improves both ride comfort and road holding.

  1. Instrumentation bus systems and communication methods for DNBPS fast controller

    International Nuclear Information System (INIS)

    Diagnostics Neutral Beam (DNB) injector requires different type of controllable power supplies, to be operated in predefined sequence; hence demands for a complex control system. Instrumentation and control (I and C) is to be opted considering design feasibility and maintainability over a long life span of experiment. One of the most important standards for I and C is the computer bus, which is needed for the selection of input and output (I/O) devices, CPU boards and other components. This paper summarizes the various I/O bus and communication methods among interconnected sub systems with reference to DNB power supply control system. (author)

  2. Persecution as a Theme in Isaac Bashevis Singer's The Bus

    Institute of Scientific and Technical Information of China (English)

    Wu Minzhi

    2008-01-01

    Isaac Bashevis Singer is very much concerned about the fate of Jewish people,so he often presents Jews'sufferings in his literary works.The Bus,by means of describing the narrator's experiences during a bus tour through Spain,in different dimensions reinforces the destructive impact of persecution that Jews have gone through.Moreover,he implies such persecution is not merely confined between Jews and the Gentile,but also spreads among the whole human beings who hold differet beliefs.

  3. DNA splice site sequences clustering method for conservativeness analysis

    Institute of Scientific and Technical Information of China (English)

    Quanwei Zhang; Qinke Peng; Tao Xu

    2009-01-01

    DNA sequences that are near to splice sites have remarkable conservativeness,and many researchers have contributed to the prediction of splice site.In order to mine the underlying biological knowledge,we analyze the conservativeness of DNA splice site adjacent sequences by clustering.Firstly,we propose a kind of DNA splice site sequences clustering method which is based on DBSCAN,and use four kinds of dissimilarity calculating methods.Then,we analyze the conservative feature of the clustering results and the experimental data set.

  4. Genome-wide analysis of alternative splicing in Chlamydomonas reinhardtii

    Directory of Open Access Journals (Sweden)

    Thomas Julie

    2010-02-01

    Full Text Available Abstract Background Genome-wide computational analysis of alternative splicing (AS in several flowering plants has revealed that pre-mRNAs from about 30% of genes undergo AS. Chlamydomonas, a simple unicellular green alga, is part of the lineage that includes land plants. However, it diverged from land plants about one billion years ago. Hence, it serves as a good model system to study alternative splicing in early photosynthetic eukaryotes, to obtain insights into the evolution of this process in plants, and to compare splicing in simple unicellular photosynthetic and non-photosynthetic eukaryotes. We performed a global analysis of alternative splicing in Chlamydomonas reinhardtii using its recently completed genome sequence and all available ESTs and cDNAs. Results Our analysis of AS using BLAT and a modified version of the Sircah tool revealed AS of 498 transcriptional units with 611 events, representing about 3% of the total number of genes. As in land plants, intron retention is the most prevalent form of AS. Retained introns and skipped exons tend to be shorter than their counterparts in constitutively spliced genes. The splice site signals in all types of AS events are weaker than those in constitutively spliced genes. Furthermore, in alternatively spliced genes, the prevalent splice form has a stronger splice site signal than the non-prevalent form. Analysis of constitutively spliced introns revealed an over-abundance of motifs with simple repetitive elements in comparison to introns involved in intron retention. In almost all cases, AS results in a truncated ORF, leading to a coding sequence that is around 50% shorter than the prevalent splice form. Using RT-PCR we verified AS of two genes and show that they produce more isoforms than indicated by EST data. All cDNA/EST alignments and splice graphs are provided in a website at http://combi.cs.colostate.edu/as/chlamy. Conclusions The extent of AS in Chlamydomonas that we observed is much

  5. Adenosine to Inosine editing frequency controlled by splicing efficiency.

    Science.gov (United States)

    Licht, Konstantin; Kapoor, Utkarsh; Mayrhofer, Elisa; Jantsch, Michael F

    2016-07-27

    Alternative splicing and adenosine to inosine (A to I) RNA-editing are major factors leading to co- and post-transcriptional modification of genetic information. Both, A to I editing and splicing occur in the nucleus. As editing sites are frequently defined by exon-intron basepairing, mRNA splicing efficiency should affect editing levels. Moreover, splicing rates affect nuclear retention and will therefore also influence the exposure of pre-mRNAs to the editing-competent nuclear environment. Here, we systematically test the influence of splice rates on RNA-editing using reporter genes but also endogenous substrates. We demonstrate for the first time that the extent of editing is controlled by splicing kinetics when editing is guided by intronic elements. In contrast, editing sites that are exclusively defined by exonic structures are almost unaffected by the splicing efficiency of nearby introns. In addition, we show that editing levels in pre- and mature mRNAs do not match. This phenomenon can in part be explained by the editing state of an RNA influencing its splicing rate but also by the binding of the editing enzyme ADAR that interferes with splicing. PMID:27112566

  6. Functional roles of alternative splicing factors in human disease.

    Science.gov (United States)

    Cieply, Benjamin; Carstens, Russ P

    2015-01-01

    Alternative splicing (AS) is an important mechanism used to generate greater transcriptomic and proteomic diversity from a finite genome. Nearly all human gene transcripts are alternatively spliced and can produce protein isoforms with divergent and even antagonistic properties that impact cell functions. Many AS events are tightly regulated in a cell-type or tissue-specific manner, and at different developmental stages. AS is regulated by RNA-binding proteins, including cell- or tissue-specific splicing factors. In the past few years, technological advances have defined genome-wide programs of AS regulated by increasing numbers of splicing factors. These splicing regulatory networks (SRNs) consist of transcripts that encode proteins that function in coordinated and related processes that impact the development and phenotypes of different cell types. As such, it is increasingly recognized that disruption of normal programs of splicing regulated by different splicing factors can lead to human diseases. We will summarize examples of diseases in which altered expression or function of splicing regulatory proteins has been implicated in human disease pathophysiology. As the role of AS continues to be unveiled in human disease and disease risk, it is hoped that further investigations into the functions of numerous splicing factors and their regulated targets will enable the development of novel therapies that are directed at specific AS events as well as the biological pathways they impact. PMID:25630614

  7. Has the natural gas fueled bus any future?

    International Nuclear Information System (INIS)

    Helsinki City Transport has decided to operate public transport in the center of the city with tramways and gas-fuelled busses. The decision is that there will be about 100 natural gas fueled busses in Helsinki by the year 2003. European exhaust gas emission (NOx and particulates) regulations have tightened strongly during the past few years. The regulations have forced to search for new fuels by the side of development of diesel engines. Alcohols, in spite of favourable fuel properties, are too expensive, so the use of them needs large subsidies for transportation sector. Gaseous fuels, both LPG and natural gas are suitable fuels for Otto cycle-cycle engines. After the previous oil crisis the interest in gas-fuelled engines has steadily decreased, but at present it is increasing again because of the objectives to decrease emissions of heavy vehicles at the level of gasoline-fuelled vehicles, equipped with three-way catalyst. From the point of view of emissions natural gas and LPG are seen as equivalent alternatives. The price of LPG varies on the basis of demand and on the basis of the prices of other oil products. Refuelling of a vehicle and storage of LPG in liquid form in the tank of the vehicle is easier than refuelling and fuel storage of natural gas. Investments to refuelling equipment of LPG are only 20% of those of the natural gas refuelling systems. The problem of natural gas is also the fact that is not easy to carry in the vehicle. Even if natural gas is compressed to pressure of 200 bars, it requires six times larger tanks if the refuelling intervals are the same. Liquefaction of natural gas reduces the volume significantly, but this is complicated and hence expensive. The tank of a vehicle should be vacuum insulated because the temperature of the LNG is about 160 deg C. Tank volume of LPG is only about twice that of diesel oil. Safety of natural gas is high, because it is lighter than the air, nearly a half of the density of the air. Octane ratings of

  8. Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay

    OpenAIRE

    Ni, Julie Z.; Grate, Leslie; Donohue, John Paul; Preston, Christine; Nobida, Naomi; O’Brien, Georgeann; Shiue, Lily; Clark, Tyson A.; Blume, John E; Ares, Manuel

    2007-01-01

    Many alternative splicing events create RNAs with premature stop codons, suggesting that alternative splicing coupled with nonsense-mediated decay (AS-NMD) may regulate gene expression post-transcriptionally. We tested this idea in mice by blocking NMD and measuring changes in isoform representation using splicing-sensitive microarrays. We found a striking class of highly conserved stop codon-containing exons whose inclusion renders the transcript sensitive to NMD. A genomic search for additi...

  9. Improving student bus-riding behavior through a whole-school intervention.

    OpenAIRE

    Putnam, Robert F; Handler, Marcie W; Ramirez-Platt, Christina M; Luiselli, James K

    2003-01-01

    We describe a multicomponent intervention to improve bus-riding behavior of students attending an urban public school. The intervention was developed with technical assistance consultation that emphasized collaboration among students, school personnel, and bus drivers. The primary intervention procedures were identifying appropriate behaviors during transportation ("bus rules"), training bus drivers to deliver positive reinforcement, and rewarding student performance through a weekly school-b...

  10. Bus Catcher: A Context Sensitive Prototype System for Public Transportation Users

    OpenAIRE

    Bertolotto, Michela; O'Hare, G.M.P.; Strahan, Robin; Brophy, Ailish; Martin, A.; McLoughlin, Eoin

    2002-01-01

    In this paper we describe the architectural and functional characteristics of Bus Catcher, a context sensitive prototype system for public transportation users. Bus Catcher assists mobile users in planning their bus rides by providing timely and accurate information about current bus locations and estimated arrival times. A complete report on the implementation together with a preliminary evaluation of the system is provided in this paper.

  11. Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process

    Directory of Open Access Journals (Sweden)

    Katja Meyer

    2015-07-01

    Full Text Available Alternative pre-messenger RNA splicing in higher plants emerges as an important layer of regulation upon exposure to exogenous and endogenous cues. Accordingly, mutants defective in RNA-binding proteins predicted to function in the splicing process show severe phenotypic alterations. Among those are developmental defects, impaired responses to pathogen threat or abiotic stress factors, and misregulation of the circadian timing system. A suite of splicing factors has been identified in the model plant Arabidopsis thaliana. Here we summarize recent insights on how defects in these splicing factors impair plant performance.

  12. Genetic Variation of Pre-mRNA Alternative Splicing in Human Populations

    OpenAIRE

    Lu, Zhi-xiang; Jiang, Peng; Xing, Yi

    2011-01-01

    The precise splicing outcome of a transcribed gene is controlled by complex interactions between cis regulatory splicing signals and trans-acting regulators. In higher eukaryotes, alternative splicing is a prevalent mechanism for generating transcriptome and proteome diversity. Alternative splicing can modulate gene function, affect organismal phenotype and cause disease. Common genetic variation that affects splicing regulation can lead to differences in alternative splicing between human in...

  13. The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing

    OpenAIRE

    Long Ma; Xiaoyang Gao; Jintao Luo; Liange Huang; Yanling Teng; H Robert Horvitz

    2012-01-01

    RNA splicing is a major regulatory mechanism for controlling eukaryotic gene expression. By generating various splice isoforms from a single pre-mRNA, alternative splicing plays a key role in promoting the evolving complexity of metazoans. Numerous splicing factors have been identified. However, the in vivo functions of many splicing factors remain to be understood. In vivo studies are essential for understanding the molecular mechanisms of RNA splicing and the biology of numerous RNA splicin...

  14. Dynamically possible pattern speeds of double bars

    CERN Document Server

    Maciejewski, Witold

    2009-01-01

    The method to study oscillating potentials of double bars, based on invariant loops, is introduced here in a new way, intended to be more intelligible. Using this method, I show how the orbital structure of a double-barred galaxy (nested bars) changes with the variation of nuclear bar's pattern speed. Not all pattern speeds are allowed when the inner bar rotates in the same direction as the outer bar. Below certain minimum pattern speed orbital support for the inner bar abruptly disappears, while high values of this speed lead to loops that are increasingly round. For values between these two extremes, loops supporting the inner bar extend further out as its pattern speed decreases, and they become more eccentric and pulsate more. These findings do not apply to counter-rotating inner bars.

  15. MSCT findings in a controlled bus bombing using a pig model

    DEFF Research Database (Denmark)

    Hansen, Nikolaj Friis

    2009-01-01

    pig's position in the bus at the time of detonation. 1 of the pigs was rigged as a suicide bomber while the others were individually placed as passengers and bus driver inside the bus alongside dummies. Measurements of the blast force and fast speed video recordings were made during the detonation...

  16. Bus Operations for Pupil Transportation. "Chrome-Yellow and Red Lights."

    Science.gov (United States)

    Rannels, Morris W.

    This paper is based on a committee report of the last National School Bus Conference on "Minimum Standards for School Buses." Standards for school bus operations are included for (1) administrators, (2) supervisors, (3) school bus drivers, (4) student passengers, and (5) maintenance and service personnel. The report deals also with procedures for…

  17. DETERMINATION OF THE DELAY DEPARTURE TIME OF THE BUS FROM STOPPING POINT INTO THE CAR FLOW

    Directory of Open Access Journals (Sweden)

    P. Gorbachov

    2014-10-01

    Full Text Available An analytical model for estimation of the bus departure delax from the bus stopping point after passenger loading is presented. The model allows to take into account the traffic density on a particular road section of the transport network, as well as the bus parameters.

  18. 46 CFR 112.05-3 - Main-emergency bus-tie.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Main-emergency bus-tie. 112.05-3 Section 112.05-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS General § 112.05-3 Main-emergency bus-tie. Each bus-tie between a main...

  19. Altered PLP1 splicing causes hypomyelination of early myelinating structures

    Science.gov (United States)

    Kevelam, Sietske H; Taube, Jennifer R; van Spaendonk, Rosalina M L; Bertini, Enrico; Sperle, Karen; Tarnopolsky, Mark; Tonduti, Davide; Valente, Enza Maria; Travaglini, Lorena; Sistermans, Erik A; Bernard, Geneviève; Catsman-Berrevoets, Coriene E; van Karnebeek, Clara D M; Østergaard, John R; Friederich, Richard L; Fawzi Elsaid, Mahmoud; Schieving, Jolanda H; Tarailo-Graovac, Maja; Orcesi, Simona; Steenweg, Marjan E; van Berkel, Carola G M; Waisfisz, Quinten; Abbink, Truus E M; van der Knaap, Marjo S; Hobson, Grace M; Wolf, Nicole I

    2015-01-01

    Objective The objective of this study was to investigate the genetic etiology of the X-linked disorder “Hypomyelination of Early Myelinating Structures” (HEMS). Methods We included 16 patients from 10 families diagnosed with HEMS by brain MRI criteria. Exome sequencing was used to search for causal mutations. In silico analysis of effects of the mutations on splicing and RNA folding was performed. In vitro gene splicing was examined in RNA from patients’ fibroblasts and an immortalized immature oligodendrocyte cell line after transfection with mutant minigene splicing constructs. Results All patients had unusual hemizygous mutations of PLP1 located in exon 3B (one deletion, one missense and two silent), which is spliced out in isoform DM20, or in intron 3 (five mutations). The deletion led to truncation of PLP1, but not DM20. Four mutations were predicted to affect PLP1/DM20 alternative splicing by creating exonic splicing silencer motifs or new splice donor sites or by affecting the local RNA structure of the PLP1 splice donor site. Four deep intronic mutations were predicted to destabilize a long-distance interaction structure in the secondary PLP1 RNA fragment involved in regulating PLP1/DM20 alternative splicing. Splicing studies in fibroblasts and transfected cells confirmed a decreased PLP1/DM20 ratio. Interpretation Brain structures that normally myelinate early are poorly myelinated in HEMS, while they are the best myelinated structures in Pelizaeus–Merzbacher disease, also caused by PLP1 alterations. Our data extend the phenotypic spectrum of PLP1-related disorders indicating that normal PLP1/DM20 alternative splicing is essential for early myelination and support the need to include intron 3 in diagnostic sequencing. PMID:26125040

  20. The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

    NARCIS (Netherlands)

    Sugliani, M.; Brambilla, V.; Clerkx, E.J.M.; Koornneef, M.; Soppe, W.J.J.

    2010-01-01

    ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3- and ABI3-ß, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-ß transcript a

  1. Bussikontsern Go Bus ihaldab Harjumaa turgu / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2005-01-01

    AS-i Tarbus ja kaheksa bussiettevõtte ühinemisel moodustati AS Go Bus, ettevõte plaanib veel mitme bussifirma liitmist. Atko Grupi juhatuse esimehe Margo Tomingase, AS Sebe nõukogu esimehe Hugo Osula arvamus. Lisa: Konkurentsiamet hoiab silma peal

  2. Platform Screen Doors Enhanced Bus Rapid Transit Intelligent Performance

    Directory of Open Access Journals (Sweden)

    Chonghua Zhou

    2011-06-01

    Full Text Available It is the last straw to clutch at to solve the urban traffic issue, to develop high-capacity rapid transit and promote transit priority. Characterized by low cost, short construction cycle and flexible development, Bus Rapid Transit (BRT has been favored by more and more cities in the world. The Platform Screen Doors (PSDs system is an important component at BRT stations, and it is original motive to be designed to satisfy the growing demand from BRT application to provide increased safety and comfort in the first. With the continual increased demand for BRT intelligent performance, the PSDs system is applied to get Bus Location Information with accurate position of arrival and departure at stop, to provide Real-Time Information (RTI for BRT passengers using PSDs/GPS compound location technology, to put into practice Bus Fleet Management (BFM. Considering the capability of accurate location, it can be applied to actualize Bus Sign Priority in the future. The authors are luck to take in part the practice of BRT system, especially in the BRT intelligent systems, the papers will introduce upwards application and conceive in detail.

  3. Sustainable transport and the organisation of bus services in Manchester

    DEFF Research Database (Denmark)

    Gudmundsson, Henrik; Sørensen, Claus Hedegaard

    2008-01-01

    depends on how the bus sector is organised. By organisation we adopt an institutional point of view and consider basic modes of governance. Basic modes of governance include the forms “market”, “hierarchy” and “network” (Powell 1990). So-called New Public Management (NPM) reforms have changed the modes of...

  4. What's Happening on the School Bus Performance Front?

    Science.gov (United States)

    Combs, Hanford L.

    The most urgent order of business in the national scene is to complete the development of a series of new performance tests for school bus bodies and chassis to establish a benchmark for safe construction that can be verified by independent testing agencies. Such performance tests should incorporate the new Federal Joint Strength Efficiency…

  5. Low back trouble among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1989-01-01

    questionnaire in 1978 regarding psychosocial factors and health. The prevalence of frequent low back pain was 57%. In a control group of 195 motormen the prevalence was 40%. Standardized Morbidity Ratio for bus drivers discharged from hospital during the period 1978-1984 with the diagnosis lumbar disc...

  6. A Novel Time Synchronization Method for Dynamic Reconfigurable Bus

    Directory of Open Access Journals (Sweden)

    Zhang Weigong

    2016-01-01

    Full Text Available UM-BUS is a novel dynamically reconfigurable high-speed serial bus for embedded systems. It can achieve fault tolerance by detecting the channel status in real time and reconfigure dynamically at run-time. The bus supports direct interconnections between up to eight master nodes and multiple slave nodes. In order to solve the time synchronization problem among master nodes, this paper proposes a novel time synchronization method, which can meet the requirement of time precision in UM-BUS. In this proposed method, time is firstly broadcasted through time broadcast packets. Then, the transmission delay and time deviations via three handshakes during link self-checking and channel detection can be worked out referring to the IEEE 1588 protocol. Thereby, each node calibrates its own time according to the broadcasted time. The proposed method has been proved to meet the requirement of real-time time synchronization. The experimental results show that the synchronous precision can achieve a bias less than 20 ns.

  7. Measuring Bus Drivers' Occupational Stress Under Changing Working Conditions

    NARCIS (Netherlands)

    Hlotova, Y.; Cats, O.; Meijer, S.A.

    2014-01-01

    Stress is an immense problem in modern society; approximately half of all occupational illnesses are directly or indirectly related to stress. The work of a bus driver is typically associated with high stress levels that negatively influence individual well-being as well as workforce management. The

  8. Measurements on and simulations of a biogas-fuelled bus

    International Nuclear Information System (INIS)

    Mathematical models for torque/fuel consumption and emissions have been studied for a biogas-fuelled bus used in urban traffic in Linkoeping, Sweden. It is noted that the cycle ECE R-49 is not representative for city buses, but that the Braunschweig cycle gives rather good agreement. 2 refs, 37 figs

  9. High-Tech School Bus Teaches Students on the Road

    Science.gov (United States)

    Katims, Lauren

    2011-01-01

    Last year, kindergarten through high school students in the rural Hector, Arkansas, School District barely had the technology resources that keep kids interested in math and science. This year, they potentially have the most advanced resources in the country--before they even step into the classroom. One school bus in Arkansas' Pope County has…

  10. Splice connector with internal heat transfer jacket

    Science.gov (United States)

    Silva, Frank A.; Mayer, Robert W.

    1977-01-01

    A heat transfer jacket is placed over the terminal portions of the conductors of a pair of high voltage cables which are connected in a splice connection wherein a housing surrounds the connected conductor portions, the heat transfer jacket extending longitudinally between the confronting ends of a pair of adaptor sleeves placed upon the insulation of the cables to engage and locate the adaptor sleeves relative to one another, and laterally between the conductors and the housing to provide a path of relatively high thermal conductivity between the connected conductor portions and the housing.

  11. Real life testing of a Hybrid PEM Fuel Cell Bus

    Science.gov (United States)

    Folkesson, Anders; Andersson, Christian; Alvfors, Per; Alaküla, Mats; Overgaard, Lars

    Fuel cells produce low quantities of local emissions, if any, and are therefore one of the most promising alternatives to internal combustion engines as the main power source in future vehicles. It is likely that urban buses will be among the first commercial applications for fuel cells in vehicles. This is due to the fact that urban buses are highly visible for the public, they contribute significantly to air pollution in urban areas, they have small limitations in weight and volume and fuelling is handled via a centralised infrastructure. Results and experiences from real life measurements of energy flows in a Scania Hybrid PEM Fuel Cell Concept Bus are presented in this paper. The tests consist of measurements during several standard duty cycles. The efficiency of the fuel cell system and of the complete vehicle are presented and discussed. The net efficiency of the fuel cell system was approximately 40% and the fuel consumption of the concept bus is between 42 and 48% lower compared to a standard Scania bus. Energy recovery by regenerative braking saves up 28% energy. Bus subsystems such as the pneumatic system for door opening, suspension and brakes, the hydraulic power steering, the 24 V grid, the water pump and the cooling fans consume approximately 7% of the energy in the fuel input or 17% of the net power output from the fuel cell system. The bus was built by a number of companies in a project partly financed by the European Commission's Joule programme. The comprehensive testing is partly financed by the Swedish programme "Den Gröna Bilen" (The Green Car). A 50 kW el fuel cell system is the power source and a high voltage battery pack works as an energy buffer and power booster. The fuel, compressed hydrogen, is stored in two high-pressure stainless steel vessels mounted on the roof of the bus. The bus has a series hybrid electric driveline with wheel hub motors with a maximum power of 100 kW. Hybrid Fuel Cell Buses have a big potential, but there are

  12. Characterisation of an urban bus network for environmental purposes.

    Science.gov (United States)

    André, Michel; Villanova, André

    2004-12-01

    Since pollutant emissions are closely related to the operating conditions of vehicles, their evaluation usually involves studying these operating conditions (through bus instrumentation and monitoring under actual operation), the design of representative driving or engine test cycles and the measurement of pollutant emissions. A preliminary characterisation of the routes on a bus network should make it possible to identify typical routes, the driving conditions and pollutant emissions of which are then studied. Two approaches are envisaged and applied to the Paris area, for which a wealth of information is available, which should be transferable to other bus networks. Both approaches are based on factorial analysis and automatic clustering, to allow optimum description and the identification of a pertinent typology of the bus routes in several classes. The first attempt at characterisation is based on statistics relating to bus operations: route characteristics (length, dedicated bus lanes, number of stops, location of stops: schools, tourist sites, hospitals, railways or underground stations), travel time, commercial speed, annual statistics (number of passengers, number of vehicles per hour, total kilometres), the irregularity of travel (variation of travel times, injuries, congestion.), as well as information on the problems encountered (congestion, distribution of the passenger load, junctions, bends). A second approach is based on the analysis of the "urban context" in which buses are driven. Population, employment, housing, road network, traffic and places that generate or disturb traffic (schools, railway stations, shopping areas, etc.) are calculated for the Ile de France region, by cells of 100 x 100 m, and collected in a geographical information system (GIS). Statistical analyses enable a typology of these urban cells to be established, the main parameters being density, type of housing, road types and traffic levels. The bus routes are then analysed

  13. Energy Performance Optimization of a Bus for Urban Public Transport

    Directory of Open Access Journals (Sweden)

    Paola Gori

    2013-08-01

    Full Text Available Nowadays many people use public transports in urban centres. Consequently, every day a lot of buses move within cities, trying to ensure the best service to citizens. During the year buses becomecrowded places and using an air conditioning system in constant operation, it tries to ensure a certain condition of comfort for the passengers on board. The aim of this study is to analyse and optimize the energy performance of a bus shell, identifying practical solutions that have not yet been adopted in order to reduce the impact of air conditioning on bus consumption and, therefore, on air pollution. For this reason it was decided to conduct a thermal analysis of a bus for public transport, in order to understandthe behaviour of the bus shell and to deduce possible optimization measures that have not yet been made until now. The analysis was carried out considering the hottest day of July and the coldest day ofJanuary, considering the conditions of operation based on the most common graphics TGM able to define the concentration of traffic city during the 24 hours per day. The study was done using the dynamicsimulation software TRNSYS. With this software it was possible to recreate faithfully the structure of the bus and the external environmental conditions, assessing the impact of different technical solutions for an improvement of internal conditions and a reduction of the cooling capacity required. Because the presence of passengers in public transport is considered like a “benefit” during the winter, the analysis started with the identification of a summer solution and the subsequent evaluation of this solution for the wintertime. The aim of this study was to optimize the bus shell and select the most appropriate solutions. Regarding the transparent surfaces it has been given importance to factors such as the thermal transmittance and the solar gain factor (g-value. Aware of the influence given by the solar radiation on the energy loads, we

  14. GeoBus: sharing science research with schools

    Science.gov (United States)

    Roper, Kathryn; Robinson, Ruth; Moorhouse, Ben

    2016-04-01

    GeoBus (www.geobus.org.uk) is an educational outreach project that was developed in 2012 by the Department of Earth and Environmental Sciences at the University of St Andrews, and it is currently sponsored by industry, NERC, The Crown Estate, and the Scottish Government. The aims of GeoBus are to support the teaching of Earth Science in secondary (middle and high) schools by providing teaching support to schools that have little or no experience in teaching this subject. This is, in part, done through the sharing of new science research outcomes and the experiences of young researchers with school pupils to provide a bridge between industry, higher education institutions, research councils and schools. Since its launch, over 40,000 pupils will have been involved in experiential Earth science learning activities in 190 different schools (over 400 separate visits) across the length and breadth of Scotland: many of these schools are in remote and disadvantaged regions. A new GeoBus project is under development within the Department of Earth Sciences at UCL in London. A key aim of GeoBus is to incorporate new research into our workshops with the main challenge being the development of appropriate resources that incorporate the key learning aims and requirements of the science and geography curricula. GeoBus works closely with researchers, teachers and educational practitioners to tailor the research outcomes to the curricula as much as possible. Over the past four years, GeoBus has developed 17 workshops, 5 challenge events and extensive field trips and each of these activities are trialled and evaluated within the university, and adjustments are made before the activities are delivered in schools. Activities are continually reviewed and further developments are made in response to both teacher and pupil feedback. This critical reflection of the project's success and impact is important to insure a positive and significant contribution to the science learning in

  15. Bars in the Barkor Street

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In daytime,the Barkor Street is bustling with tourists,pious pilgrims and merchants crowded together.Only when darkness falls does the street become tranquil.Nevertheless,the area surrounding the Barkor Street(from Tibetan Hospital compound extending to east Beijing Road)is then revived by various kinds of bars.

  16. Bars in Beijing Old Town

    Institute of Scientific and Technical Information of China (English)

    GUANYUNZHANG

    2003-01-01

    It's seven pm on a summer's evening by Shichahao Lake,central Beijing.A welcome breeze whispers across the lake,and the ruddy vestiges of sunset linger on roofs and treetops.A low hum emanates from bars on the lakeshore packed with young people chatting,drinking and generally

  17. Exon Expression and Alternatively Spliced Genes in Tourette Syndrome

    NARCIS (Netherlands)

    Tian, Yingfang; Liao, Isaac H.; Zhan, Xinhua; Gunther, Joan R.; Ander, Bradley P.; Liu, Dazhi; Lit, Lisa; Jickling, Glen C.; Corbett, Blythe A.; Bos-Veneman, Netty G. P.; Hoekstra, Pieter J.; Sharp, Frank R.

    2011-01-01

    Tourette Syndrome (TS) is diagnosed based upon clinical criteria including motor and vocal tics. We hypothesized that differences in exon expression and splicing might be useful for pathophysiology and diagnosis. To demonstrate exon expression and alternatively spliced gene differences in blood of i

  18. A study of alternative splicing in the pig

    DEFF Research Database (Denmark)

    Hillig, Ann-Britt Nygaard; Cirera Salicio, Susanna; Gilchrist, Michael J.;

    2010-01-01

    BACKGROUND: Since at least half of the genes in mammalian genomes are subjected to alternative splicing, alternative pre-mRNA splicing plays an important contribution to the complexity of the mammalian proteome. Expressed sequence tags (ESTs) provide evidence of a great number of possible alterna...

  19. Synaptic signaling and aberrant RNA splicing in autism spectrum disorders

    OpenAIRE

    Ryan M Smith; Wolfgang eSadee

    2011-01-01

    Interactions between presynaptic and postsynaptic cellular adhesion molecules drive synapse maturation during development. These trans-synaptic interactions are regulated by alternative splicing of cellular adhesion molecule RNAs, which ultimately determines neurotransmitter phenotype. The diverse assortment of RNAs produced by alternative splicing generates countless protein isoforms necessary for guiding specialized cell-to-cell connectivity. Failure to generate the appropriate synaptic ...

  20. Minor class splicing shapes the zebrafish transcriptome during development

    DEFF Research Database (Denmark)

    Markmiller, Sebastian; Cloonan, Nicole; Lardelli, Rea M;

    2014-01-01

    Minor class or U12-type splicing is a highly conserved process required to remove a minute fraction of introns from human pre-mRNAs. Defects in this splicing pathway have recently been linked to human disease, including a severe developmental disorder encompassing brain and skeletal abnormalities...

  1. Synaptic Signaling and Aberrant RNA Splicing in Autism Spectrum Disorders

    OpenAIRE

    Smith, Ryan M; Sadee, Wolfgang

    2011-01-01

    Interactions between presynaptic and postsynaptic cellular adhesion molecules (CAMs) drive synapse maturation during development. These trans-synaptic interactions are regulated by alternative splicing of CAM RNAs, which ultimately determines neurotransmitter phenotype. The diverse assortment of RNAs produced by alternative splicing generates countless protein isoforms necessary for guiding specialized cell-to-cell connectivity. Failure to generate the appropriate synaptic adhesion proteins i...

  2. Quantitative regulation of alternative splicing in evolution and development

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Roy, Scott W;

    2009-01-01

    Alternative splicing (AS) is a widespread mechanism with an important role in increasing transcriptome and proteome diversity by generating multiple different products from the same gene. Evolutionary studies of AS have focused primarily on the conservation of alternatively spliced sequences or of...... additional layer in complex gene regulatory networks and in the emergence of genetic novelties....

  3. Tissue-specific splicing factor gene expression signatures

    NARCIS (Netherlands)

    A.R. Grosso; A.Q. Gomes (Anita); N.L. Barbosa-Morais (Nuno); S. Caldeira (Sandra); N.P. Thorne (Natalie); G. Grech (Godfrey); M.M. von Lindern (Marieke); M. Carmo-Fonseca (Maria)

    2008-01-01

    textabstractThe alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that tissue-spec

  4. Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals.

    OpenAIRE

    Deshler, J O; Larson, G P; Rossi, J J

    1989-01-01

    The actin (ACT) gene from the budding yeast Kluyveromyces lactis was cloned, and the nucleotide sequence was determined. The gene had a single intron 778 nucleotides in length which possessed the highly conserved splicing signals found in Saccharomyces cerevisiae introns. We demonstrated splicing of heterologous ACT transcripts in both K. lactis and S. cerevisiae.

  5. Galaxy Zoo: Observing Secular Evolution Through Bars

    CERN Document Server

    Cheung, Edmond; Masters, Karen L; Nichol, Robert C; Bosma, A; Bell, Eric F; Faber, S M; Koo, David C; Lintott, Chris; Melvin, Thomas; Schawinski, Kevin; Skibba, Ramin A; Willett, Kyle W

    2013-01-01

    Observations have shown that there is a connection between the presence of a bar and the properties of a galaxy. In a parallel effort, simulations have shown that this connection is consistent with the theory of bar-driven secular evolution. But observational evidence of bar-driven secular evolution has been sparse. In this paper, we use the Galaxy Zoo 2 dataset to look for evidence of this secular evolution. Our sample consists of 13,295 disk galaxies, with an overall bar fraction of 23.6 +/- 0.4%, of which 1,154 barred galaxies also have bar length measurements. These samples are the largest ever used to study the role of bars in disk galaxy evolution. We characterize bars by the bar likelihood, the likelihood a bar is present in a given galaxy, and the bar length. These two bar properties show interesting correlations with the specific star formation rate and the inner central structure of galaxies. Comparing these observations to state-of-the-art simulations of bar evolution, which include live halos and ...

  6. Splice Site Mutations in the ATP7A Gene

    DEFF Research Database (Denmark)

    Skjørringe, Tina; Tümer, Zeynep; Møller, Lisbeth Birk

    2011-01-01

    Menkes disease (MD) is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12...... mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation to in...... vivo results. Ninety-six percent of the mutations identified in 45 patients with classical MD were predicted to have a significant effect on splicing, which concurs with the absence of any detectable wild-type transcript in all 19 patients investigated in vivo. Sixty-seven percent of the mutations...

  7. Comparative Analysis of Splice Site Regions by Information Content

    Institute of Scientific and Technical Information of China (English)

    T. Shashi Rekha; Chanchal K. Mitra

    2006-01-01

    We have applied concepts from information theory for a comparative analysis of donor (gt) and acceptor (ag) splice site regions in the genes of five different organisms by calculating their mutual information content (relative entropy) over a selected block of nucleotides. A similar pattern that the information content decreases as the block size increases was observed for both regions in all the organisms studied. This result suggests that the information required for splicing might be contained in the consensus of ~6-8 nt at both regions. We assume from our study that even though the nucleotides are showing some degrees of conservation in the flanking regions of the splice sites, certain level of variability is still tolerated,which leads the splicing process to occur normally even if the extent of base pairing is not fully satisfied. We also suggest that this variability can be compensated by recognizing different splice sites with different spliceosomal factors.

  8. An Optimization Model for the Selection of Bus-Only Lanes in a City

    OpenAIRE

    Chen, Qun

    2015-01-01

    The planning of urban bus-only lane networks is an important measure to improve bus service and bus priority. To determine the effective arrangement of bus-only lanes, a bi-level programming model for urban bus lane layout is developed in this study that considers accessibility and budget constraints. The goal of the upper-level model is to minimize the total travel time, and the lower-level model is a capacity-constrained traffic assignment model that describes the passenger flow assignment ...

  9. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-03-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This report provides the early data results and implementation experience of the AT fuel cell bus since it was placed in service.

  10. Improving student bus-riding behavior through a whole-school intervention.

    Science.gov (United States)

    Putnam, Robert F; Handler, Marcie W; Ramirez-Platt, Christina M; Luiselli, James K

    2003-01-01

    We describe a multicomponent intervention to improve bus-riding behavior of students attending an urban public school. The intervention was developed with technical assistance consultation that emphasized collaboration among students, school personnel, and bus drivers. The primary intervention procedures were identifying appropriate behaviors during transportation ("bus rules"), training bus drivers to deliver positive reinforcement, and rewarding student performance through a weekly school-based lottery. Disruptive bus behaviors, as measured by discipline referrals and suspensions, decreased with intervention relative to baseline phases in an ABAB reversal design. These positive results were maintained over the long term, with school personnel assuming responsibility for intervention in the absence of ongoing consultation. PMID:14768676

  11. Implementation of an AMBA Advanced High Performance Bus protocol IP block

    OpenAIRE

    Mahendra Vucha Sandeep Magarde; Miss. Shruti Bhargava; Rishabh Singh Kurmi

    2011-01-01

    The design of an AMBA advanced high performance bus (AHB) protocol IP block is presented in this paper. The AHB (Advanced High-performance Bus) is a high-performance bus in AMBA (Advanced Microcontroller Bus Architecture) family. This AHB can be used in high clock frequency system modules. The AHB acts as the high-performance system backbone bus. AHB supports the efficient connection of processors, on-chip memories and off-chip external memory interfaces with low-power peripheral macro cell f...

  12. Bar, Orliac-de-Bar, Beaumont, Saint–Augustin, Ussel

    OpenAIRE

    Simonnot, Bernard

    2013-01-01

    Identifiant de l'opération archéologique : 2735 Date de l'opération : 2007 (PR) Inventeur(s) : Simonnot Bernard (BEN) La prospection sur les communes de Bar, Orliac-de-Bar, Beaumont et Saint-Augustin n’ayant pas donné pour le moment de nouveaux résultats satisfaisants, une reprise sera nécessaire lors de la prochaine campagne. Ceux découverts récemment demandent une étude plus approfondie. Cette année, la prospection s’est limitée à la partie ouest de la commune d’Ussel, dans la zone de renc...

  13. Going Smokefree Matters - Bars and Restaurants Infographic

    Data.gov (United States)

    U.S. Department of Health & Human Services — Explore the Going Smokefree Matters – Bars and Restaurants Infographic which outlines key facts related to the effects of secondhand smoke exposure in bars and...

  14. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Roy, Scott William

    2009-01-01

    interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs) on exon inclusion levels in human and chimpanzee. For this purpose, we...... compiled and studied comprehensive datasets of predicted ESRs, identified by several computational and experimental approaches, as well as microarray data for changes in alternative splicing levels between human and chimpanzee. Surprisingly, we found no association between changes in predicted ESRs and...... or no effect on splicing, and thus interspecific changes at short-time scales may primarily occur in these effectively neutral ESRs. These results underscore the difficulties of using current computational ESR prediction algorithms to identify truly functionally important motifs, and provide a...

  15. Design of a CAN bus interface for photoelectric encoder in the spaceflight camera

    Science.gov (United States)

    Sun, Ying; Wan, Qiu-hua; She, Rong-hong; Zhao, Chang-hai; Jiang, Yong

    2009-05-01

    In order to make photoelectric encoder usable in a spaceflight camera which adopts CAN bus as the communication method, CAN bus interface of the photoelectric encoder is designed in this paper. CAN bus interface hardware circuit of photoelectric encoder consists of CAN bus controller SJA 1000, CAN bus transceiver TJA1050 and singlechip. CAN bus interface controlling software program is completed in C language. A ten-meter shield twisted pair line is used as the transmission medium in the spaceflight camera, and speed rate is 600kbps.The experiments show that: the photoelectric encoder with CAN bus interface which has the advantages of more reliability, real-time, transfer rate and transfer distance overcomes communication line's shortcomings of classical photoelectric encoder system. The system works well in automatic measuring and controlling system.

  16. Comprehensive splicing graph analysis of alternative splicing patterns in chicken, compared to human and mouse

    Directory of Open Access Journals (Sweden)

    Ranganathan Shoba

    2009-07-01

    Full Text Available Abstract Background Alternative transcript diversity manifests itself as a prime cause of complexity in higher eukaryotes. Recently, transcript diversity studies have suggested that 60–80% of human genes are alternatively spliced. We have used a splicing pattern approach for the bioinformatics analysis of Alternative Splicing (AS in chicken, human and mouse. Exons involved in splicing are subdivided into distinct and variant exons, based on the prevalence of the exons across the transcripts. Four possible permutations of these two different groups of exons were categorised as class I (distinct-variant, class II (distinct-variant, class III (variant-distinct and class IV (variant-variant. This classification quantifies the variation in transcript diversity in the three species. Results In all, 3901 chicken AS genes have been compared with 16,715 human and 16,491 mouse AS genes, with 23% of chicken genes being alternatively spliced, compared to 68% in humans and 57% in mice. To minimize any gene structure bias in the input data, comparative genome analysis has been carried out on the orthologous subset of AS genes for the three species. Gene-level analysis suggested that chicken genes show fewer AS events compared to human and mouse. An event-level analysis showed that the percentage of AS events in chicken is similar to that of human, which implies that a smaller number of chicken genes show greater transcript diversity. Overall, chicken genes were found to have fewer transcripts per gene and shorter introns than human and mouse genes. Conclusion In chicken, the majority of genes generate only two or three isoforms, compared to almost eight in human and six in mouse. We observed that intron definition is expressed strongly when compared to exon definition for chicken genome, based on 3% intron retention in chicken, compared to 2% in human and mouse. Splicing patterns with variant exons account for 33% of AS chicken orthologous genes compared to

  17. WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo

    International Nuclear Information System (INIS)

    Wilm's tumor protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: WT1(-KTS), a transcription factor, and WT1(+KTS), a post-transcriptional regulator that binds to RNA and can interact with splicing components. Here we show that WT1 interacts with the novel splicing regulator RBM4. Each protein was found to colocalize in nuclear speckles and to cosediment with supraspliceosomes in glycerol gradients. RBM4 conferred dose-dependent and cell-specific regulation of alternative splicing of pre-mRNAs transcribed from several reporter genes. We found that overexpressed WT1(+KTS) abrogated this effect of RBM4 on splice-site selection, whereas WT1(-KTS) did not. We conclude that the (+KTS) form of WT1 is able to inhibit the effect of RBM4 on alternative splicing

  18. Bar instabilities in Coma cluster galaxies

    International Nuclear Information System (INIS)

    The radial distribution of bar versus nonbar galaxies within the Coma cluster shows that a significantly larger fraction of bar galaxies are members of the cluster core. This result can be used either to estimate the time scale for the decay of bar instabilities or to argue that galaxies in the core of Coma are confined within the core

  19. Bar code instrumentation for nuclear safeguards

    International Nuclear Information System (INIS)

    This paper presents a brief overview of the basic principles of bar codes and the equipment used to make and to read bar code labels, and a summary of some of the more important factors that need to be considered in integrating bar codes into an information system

  20. In vitro Splicing of Influenza Viral NS1 mRNA and NS1-β -globin Chimeras: Possible Mechanisms for the Control of Viral mRNA Splicing

    Science.gov (United States)

    Plotch, Stephen J.; Krug, Robert M.

    1986-08-01

    In influenza virus-infected cells, the splicing of the viral NS1 mRNA catalyzed by host nuclear enzymes is controlled so that the steady-state amount of the spliced NS2 mRNA is only 5-10% of that of the unspliced NS1 mRNA. Here we examine the splicing of NS1 mRNA in vitro, using nuclear extracts from HeLa cells. We show that in addition to its consensus 5' and 3' splice sites, NS1 mRNA has an intron branch-point adenosine residue that was functional in lariat formation. Nonetheless, this RNA was not detectably spliced in vitro under conditions in which a human β -globin precursor was efficiently spliced. Using chimeric RNA precursors containing both NS1 and β -globin sequences, we show that the NS1 5' splice site was effectively utilized by the β -globin branch-point sequence and 3' splice site to form a spliced RNA, whereas the NS1 3' splice site did not function in detectable splicing in vitro, even in the presence of the β -globin branch-point sequence or in the presence of both the branch-point sequence and 5' exon and splice site from β -globin With the chimeric precursors that were not detectably spliced, as with NS1 mRNA itself, a low level of a lariat structure containing only intron and not 3' exon sequences was formed. The inability of the consensus 3' splice site of NS1 mRNA to function effectively in in vitro splicing suggests that this site is structurally inaccessible to components of the splicing machinery. Based on these results, we propose two mechanisms whereby NS1 mRNA splicing in infected cells is controlled via the accessibility of its 3' splice site.

  1. Galaxy Zoo: Observing secular evolution through bars

    Energy Technology Data Exchange (ETDEWEB)

    Cheung, Edmond; Faber, S. M.; Koo, David C. [Department of Astronomy and Astrophysics, 1156 High Street, University of California, Santa Cruz, CA 95064 (United States); Athanassoula, E.; Bosma, A. [Aix Marseille Université, CNRS, LAM (Laboratoire d' Astrophysique de Marseille) UMR 7326, F-13388, Marseille (France); Masters, Karen L.; Nichol, Robert C.; Melvin, Thomas [Institute of Cosmology and Gravitation, University of Portsmouth, Dennis Sciama Building, Portsmouth, PO1 3FX (United Kingdom); Bell, Eric F. [Department of Astronomy, University of Michigan, 500 Church Street, Ann Arbor, MI 48109 (United States); Lintott, Chris [Oxford Astrophysics, Department of Physics, University of Oxford, Denys Wilkinson Building, Keble Road, Oxford OX1 3RH (United Kingdom); Schawinski, Kevin [Institute for Astronomy, Department of Physics, ETH Zurich, Wolfgang-Pauli-Strasse 27, CH-8093 Zurich (Switzerland); Skibba, Ramin A. [Center for Astrophysics and Space Sciences, Department of Physics, 9500 Gilman Drive, University of California, San Diego, CA 92093 (United States); Willett, Kyle W., E-mail: ec2250@gmail.com [School of Physics and Astronomy, University of Minnesota, MN 55455 (United States)

    2013-12-20

    In this paper, we use the Galaxy Zoo 2 data set to study the behavior of bars in disk galaxies as a function of specific star formation rate (SSFR) and bulge prominence. Our sample consists of 13,295 disk galaxies, with an overall (strong) bar fraction of 23.6% ± 0.4%, of which 1154 barred galaxies also have bar length (BL) measurements. These samples are the largest ever used to study the role of bars in galaxy evolution. We find that the likelihood of a galaxy hosting a bar is anticorrelated with SSFR, regardless of stellar mass or bulge prominence. We find that the trends of bar likelihood and BL with bulge prominence are bimodal with SSFR. We interpret these observations using state-of-the-art simulations of bar evolution that include live halos and the effects of gas and star formation. We suggest our observed trends of bar likelihood with SSFR are driven by the gas fraction of the disks, a factor demonstrated to significantly retard both bar formation and evolution in models. We interpret the bimodal relationship between bulge prominence and bar properties as being due to the complicated effects of classical bulges and central mass concentrations on bar evolution and also to the growth of disky pseudobulges by bar evolution. These results represent empirical evidence for secular evolution driven by bars in disk galaxies. This work suggests that bars are not stagnant structures within disk galaxies but are a critical evolutionary driver of their host galaxies in the local universe (z < 1).

  2. Galaxy Zoo: Observing secular evolution through bars

    International Nuclear Information System (INIS)

    In this paper, we use the Galaxy Zoo 2 data set to study the behavior of bars in disk galaxies as a function of specific star formation rate (SSFR) and bulge prominence. Our sample consists of 13,295 disk galaxies, with an overall (strong) bar fraction of 23.6% ± 0.4%, of which 1154 barred galaxies also have bar length (BL) measurements. These samples are the largest ever used to study the role of bars in galaxy evolution. We find that the likelihood of a galaxy hosting a bar is anticorrelated with SSFR, regardless of stellar mass or bulge prominence. We find that the trends of bar likelihood and BL with bulge prominence are bimodal with SSFR. We interpret these observations using state-of-the-art simulations of bar evolution that include live halos and the effects of gas and star formation. We suggest our observed trends of bar likelihood with SSFR are driven by the gas fraction of the disks, a factor demonstrated to significantly retard both bar formation and evolution in models. We interpret the bimodal relationship between bulge prominence and bar properties as being due to the complicated effects of classical bulges and central mass concentrations on bar evolution and also to the growth of disky pseudobulges by bar evolution. These results represent empirical evidence for secular evolution driven by bars in disk galaxies. This work suggests that bars are not stagnant structures within disk galaxies but are a critical evolutionary driver of their host galaxies in the local universe (z < 1).

  3. Splicing Express: a software suite for alternative splicing analysis using next-generation sequencing data

    OpenAIRE

    Kroll, Jose E.; Kim, JiHoon; Ohno-Machado, Lucila; de Souza, Sandro J.

    2015-01-01

    Motivation. Alternative splicing events (ASEs) are prevalent in the transcriptome of eukaryotic species and are known to influence many biological phenomena. The identification and quantification of these events are crucial for a better understanding of biological processes. Next-generation DNA sequencing technologies have allowed deep characterization of transcriptomes and made it possible to address these issues. ASEs analysis, however, represents a challenging task especially when many dif...

  4. Genome-wide survey of Alternative Splicing in Sorghum Bicolor.

    Science.gov (United States)

    Panahi, Bahman; Abbaszadeh, Bahram; Taghizadeghan, Mehdi; Ebrahimie, Esmaeil

    2014-07-01

    Sorghum bicolor is a member of grass family which is an attractive model plant for genome study due to interesting genome features like low genome size. In this research, we performed comprehensive investigation of Alternative Splicing and ontology aspects of genes those have undergone these events in sorghum bicolor. We used homology based alignments between gene rich transcripts, represented by tentative consensus (TC) transcript sequences, and genomic scaffolds to deduce the structure of genes and identify alternatively spliced transcripts in sorghum. Using homology mapping of assembled expressed sequence tags with genomics data, we identified 2,137 Alternative Splicing events in S. bicolor. Our study showed that complex events and intron retention are the main types of Alternative Splicing events in S. bicolor and highlights the prevalence of splicing site recognition for definition of introns in this plant. Annotations of the alternatively spliced genes revealed that they represent diverse biological process and molecular functions, suggesting a fundamental role for Alternative Splicing in affecting the development and physiology of S. bicolor. PMID:25049459

  5. Galaxy Zoo: Observing Secular Evolution Through Bars

    Science.gov (United States)

    Cheung, Edmond; Athanassoula, L.; Masters, K.; Faber, S. M.; Koo, D. C.; Zoo, Galaxy

    2014-01-01

    In this talk, I use the Galaxy Zoo 2 dataset to study the behavior of bars in disk galaxies as a function of specific star formation rate (SSFR), and inner galactic structure, i.e., the prominence of the bulge as parameterized by Sérsic index and central surface stellar mass density. Our sample consists of 13,295 disk galaxies, with an overall bar fraction of 23.6 ± 0.4%, of which 1,154 barred galaxies also have bar length measurements. These samples are the largest ever used to study the role of bars in disk galaxy evolution. I find that the likelihood of a galaxy hosting a bar is anti-correlated with SSFR, regardless of stellar mass or bulge prominence. I find that the trends of bar likelihood with bulge prominence are bimodal with SSFR, i.e., in star-forming galaxies, bulges are more prominent in galaxies more likely to host bars, while in quiescent disk galaxies, bars are less frequent where there are prominent bulges. Our observations of bar length reveal a complex picture. In star-forming disks, longer bars are found where the bulges are more prominent, while in quiescent disks there is a maximum in the average bar length as a function of bulge prominence. I interpret these observations using state-of-the-art simulations of bar evolution which include live halos and the effects of gas and star formation. I suggest our observed trends of bar likelihood with SSFR are driven by the gas fraction of the disks; a factor demonstrated to significantly retard both bar formation and evolution in models. I interpret the bimodal relationship between bulge prominence and bar properties as due to the complicated effects of classical bulges and central mass concentrations on bar evolution, and also to the growth of disky pseudobulges by bar evolution. These results represent empirical evidence for secular evolution driven by bars in disk galaxies. This work suggests that bars are not stagnant structures within disk galaxies, but are a critical evolutionary driver of their

  6. Search for \\bar{B}^0\\to\\Lambda_c^+\\bar{\\Lambda}_c^- decay at Belle

    OpenAIRE

    The Belle Collaboration; Abe, K

    2007-01-01

    We search for the doubly charmed baryonic decay $\\bar{B}^0\\to\\Lambda_c^+\\bar{\\Lambda}_c^-$, in a data sample of $520\\times10^6$ $B{\\bar B}$ events accumulated at the $\\Upsilon(4S)$ resonance with the Belle detector at the KEKB asymmetric e^+e^- collider. We find no significant signal and set an upper limit of ${\\cal B}(\\bar{B}^0\\to\\Lambda_c^+\\bar{\\Lambda}_c^-)

  7. Implementation of I 2C Master Bus Protocol on FPGA

    Directory of Open Access Journals (Sweden)

    Regu Archana

    2014-10-01

    Full Text Available The focus of this paper is on I 2C (Inter-Integrated Circuit protocol interface between Master Bus protocol and slave. Here we are interfacing between micro-controller and DS1307. I 2C bus protocol sends 8 bit data from micro-controller to DS1307. This module was designed in VHDL and simulated and synthesized using Xilinx ISE Design Suite 14.2. I 2C and optimized for area and power. This concept is widely applicable from any high speed device or low speed device to any low speed device or high speed device. This module acts as a slave for the DS1307 at the same time acts like a master for the micro-controller device which can be considered as a slave. . It can be used to interface low speed peripherals like motherboard, embedded system, mobile phones, set top boxes, DVD, PDA’s or other electronic devices.

  8. Design of CAN Bus Application in the Process of Papermaking

    Directory of Open Access Journals (Sweden)

    Kaisheng Zhang

    2013-05-01

    Full Text Available In this study, we have a research of the design of can bus application in the process of papermaking. The design for an intelligent measure and control system based on the technology of CAN bus, is applied to detect and control the concentration of wood-pulp, the temperature of multistage dryer and other parameters in the process of papermaking, in order to meet the control requirements and characters for the study quality and ensure paper even and cohesive under the course of manufacturer. The intelligence of measure and control system has been predominantly researched in the article, including the proposal of intelligent control node and communication model, the design of hardware interface circuit in the intelligent measure and control node and the design of whole system.

  9. Leveraging Bus Mobility to Enable Communications in Urban Areas

    CERN Document Server

    Gaito, Sabrina; Rossi, Gian Paolo

    2011-01-01

    This paper shows that the deployment of an opportunistic network on any public transportation system leads to obtain a scalable and efficient urban communication platform. We use the term Bus Switched Networks (BSNs) to indicate this urban backbone that complements the services of 3G networks and enables to meet the application level requirements for a large class of applications by ensuring high delivery ratio and acceptable delays under different conditions of packet load. We sustain these arguments by providing three contributions. The first contribution is a novel and lightweight probabilistic routing protocol for BSN which we prove to be highly effective in satisfying the loose QoS required by urban-wide delay-tolerant information services. The second contribution is the proposal of URBeS, an analysis platform that, given a specific city served by public transportation, produces real bus mobility traces and traffic analysis for any given routing protocol. The last contribution is an extensive benchmark a...

  10. Hardware Engines for Bus Encryption: A Survey of Existing Techniques

    CERN Document Server

    Elbaz, R; Sassatelli, G; Guillemin, P; Anguille, C; Bardouillet, M; Buatois, C; Rigaud, J B

    2011-01-01

    The widening spectrum of applications and services provided by portable and embedded devices bring a new dimension of concerns in security. Most of those embedded systems (pay-TV, PDAs, mobile phones, etc...) make use of external memory. As a result, the main problem is that data and instructions are constantly exchanged between memory (RAM) and CPU in clear form on the bus. This memory may contain confidential data like commercial software or private contents, which either the end-user or the content provider is willing to protect. The goal of this paper is to clearly describe the problem of processor-memory bus communications in this regard and the existing techniques applied to secure the communication channel through encryption - Performance overheads implied by those solutions will be extensively discussed in this paper.

  11. Heat storage for a bus petrol internal-combustion engine

    Science.gov (United States)

    Vasiliev, Leonard L.; Burak, Victor S.; Kulakov, Andry G.; Mishkinis, Donatas A.; Bohan, Pavel V.

    The heat storage (HS) system for pre-heating a bus petrol internal combustion engine to starting was mathematically modelled and experimentally investigated. The development of such devices is an extremely urgent problem especially for regions with a cold climate. We discuss how HS works on the effect of absorption and rejection of heat energy at a solid-liquid phase change of a HS substance. In the first part of the paper a numerical method to calculate the HS mass-dimensional parameters and their characteristics are described. In the experimental part of the paper results are given of experiments on the pre-heating device aiding to start a carburettor engine under operational conditions and analysis of data received. Practical confirmation of the theoretical development of HS devices for a bus engine for starting by pre-heating is given.

  12. StarBus+: Distributed Object Middleware Practice for Internet Computing

    Institute of Scientific and Technical Information of China (English)

    Huai-Min Wang; Yu-Feng Wang; Yang-Bin Tang

    2005-01-01

    In the past decade, the booming of Internet challenges the middleware in three aspects: quality of service,balance of changes and stabilization, and across-Internet integration. This paper presents our work on distributed object computing middleware technology for these challenges, as well as the research and development on StarBus+, which is a CORBA standard-compliant middleware suite with the features such as object request broker supporting multi-* quality of service, component model, and integration with Web Service. This paper comprehensively presents the design and characteristics of StarBns+, and demonstrates how StarBus+ is enhanced to address the challenges of Internet computing through three case studies: inter-enterprise integration over Internet, application evolution through dynamic reconfiguration,and great massive information system building. The paper also suggests some research directions which are important for Internet computing.

  13. THE DESIGN OF VMEBUS BRIDGE CONTROLLER WITH SHARC BUS

    Institute of Scientific and Technical Information of China (English)

    Wang Min; Wu Shunjun; Su Tao

    2005-01-01

    Targeting at the high expense and inflexibility to realize VMEbus bridge controller by professional Integrated Circuit (IC), this paper presents a scheme of adopting CPLD/FPGA (Complicated Programmable Logic Device/Field Programmable Gate Array) to design bridge controller between VMEbus and local bus. SHARC DSP (Digital Signal Processor) bus is an example. It has functions of nearly entire master/slave interface of VMEbus, and can act as DMA (Direct Memory Access) controller and perform block transfer in DMA or master processor initiative way without length limit. External circuit of the design is very simple. In comparison with special ICs, it has high performance to price ratio and can be easily applied to local buses of other processors with quite a little modification.

  14. Health effect of a bus ride in an urban environment

    Energy Technology Data Exchange (ETDEWEB)

    Takano, T.

    1983-07-01

    Health effects on subjects during a bus drive on an urban road were investigated in a real situation. Leukocyte count, adrenalin level in blood and urine, noradrenalin and dopamine in urine, diastolic blood pressure, and index of vascular resistance increased significantly on the drive day compared with the control day. A bus drive on an urban road induced enhanced sympathetic-adreno-medullary activity in real life as a result of the comprehensive effect of various factors. The concentrations of air pollutants were excessively high on the road, and correlated with traffic volume. It was concluded that the interactions between psychogenic, physical nonspecific and physical specific effects should be taken into consideration when evaluating human health effects of air pollution in the urban environment.

  15. Methods of Electro-Dynamic Stability Calculation of Flexible Bus-bars in High Voltage Switch Gears

    Directory of Open Access Journals (Sweden)

    I. I. Sergey

    2010-01-01

    Full Text Available The improved vector-parametrical and simplified methods for calculation of electro-dynamic stability parameters of flexible busbars in high voltage switch gears have been presented in the paper. The developed methods and their software can be taken as a basis of methodological instructions on calculation of short-circuit current electro-dynamic action on flexible conductors of switching gears.

  16. Methods of Electro-Dynamic Stability Calculation of Flexible Bus-bars in High Voltage Switch Gears

    OpenAIRE

    I. I. Sergey; Y. G. Panamarenka; A. S. Mikhalyov

    2010-01-01

    The improved vector-parametrical and simplified methods for calculation of electro-dynamic stability parameters of flexible busbars in high voltage switch gears have been presented in the paper. The developed methods and their software can be taken as a basis of methodological instructions on calculation of short-circuit current electro-dynamic action on flexible conductors of switching gears.

  17. Effect of bars on the galaxy properties

    CERN Document Server

    Vera, Matias; Coldwell, Georgina

    2016-01-01

    Aims: With the aim of assessing the effects of bars on disc galaxy properties, we present an analysis of different characteristics of spiral galaxies with strong, weak and without bars. Method: We identified barred galaxies from the Sloan Digital Sky Survey. By visual inspection, we classified the face-on spiral galaxies brighter than g<16.5 mag into strong-bar, weak-bar and unbarred. In order to provide an appropiate quantification of the influence of bars on galaxy properties, we also constructed a suitable control sample of unbarred galaxies with similar redshift, magnitude, morphology, bulge sizes, and local density environment distributions to that of barred galaxies. Results: We found 522 strong-barred and 770 weak-barred galaxies, representing a 25.82% of the full sample of spiral galaxies, in good agreement with previous studies. We also found that strong-barred galaxies show less efficient star formation activity and older stellar populations compared to weak-barred and unbarred spirals from the c...

  18. Contagion processes on urban bus networks in Indian cities

    OpenAIRE

    Chatterjee, Atanu; Ramadurai, Gitakrishnan; Jagannathan, Krishna

    2015-01-01

    Bus transportation is considered as one of the most convenient and cheapest modes of public transportation in Indian cities. Due to their cost-effectiveness and wide reachability, they help a significant portion of the human population in cities to reach their destinations every day. Although from a transportation point of view they have numerous advantages over other modes of public transportation, they also pose a serious threat of contagious diseases spreading throughout the city. The pres...

  19. Crack detection sensor layout and bus configuration analysis

    International Nuclear Information System (INIS)

    In crack detection applications large sensor arrays are needed to be able to detect and locate cracks in structures. Emerging graphene-oxide paper sensing skins are a promising technology that will help enable structural sensing skins, but in order to make use of them we must consider how the sensors will be laid out and wired on the skin. This paper analyzes different sensor shapes and layouts to determine the layout which provides the preferred performance. A ‘snaked hexagon’ layout is proposed as the preferred sensor layout when both crack detection and crack location parameters are considered. In previous work we have developed a crack detection circuit which reduces the number of channels of the system by placing several sensors onto a common bus line. This helps reduce data and power consumption requirements but reduces the robustness of the system by creating the possibility of losing sensing in several sensors in the event that a single wire breaks. In this paper, sensor bus configurations are analyzed to increase the robustness of the bused sensor system. Results show that spacing out sensors in the same bus as much as possible increases the robustness of the system and that at least 3 buses are needed to prevent large segments of a structure from losing sensing in the event of a bus failure. This work is a preliminary effort toward enabling a new class of ‘networked materials’ that will be vitally important for next generation structural applications. ‘Networked materials’ have material properties related to information theoretic concepts. An example material property is ‘bandwidth’ per unit of material that might indicate the amount of information the material can provide about its state-of-health. (paper)

  20. System investigation H2 bus fleet (technology, regulations, future development)

    International Nuclear Information System (INIS)

    In cooperation with Ansaldo Ricerche, ICIL have compiled a technical catalogue, providing the impartial descriptions, both of existing technology and regulations, and the likely future developments of these, as to remedy the first problem faced by a potential hydrogen bus fleet operator viz the absence of an impartial description of the available vehicle and fuels systems together with the absence of a description of regulatory and safety factors which need consideration. (orig.)

  1. Scheduling with Bus Access Optimization for Distributed Embedded Systems

    DEFF Research Database (Denmark)

    Eles, Petru; Doboli, Alex; Pop, Paul;

    2000-01-01

    of control. Our goal is to derive a worst case delay by which the system completes execution, such that this delay is as small as possible; to generate a logically and temporally deterministic schedule; and to optimize parameters of the communication protocol such that this delay is guaranteed. We......, generates an efficient bus access scheme as well as the schedule tables for activation of processes and communications....

  2. Leveraging Bus Mobility to Enable Communications in Urban Areas

    OpenAIRE

    Gaito, Sabrina; Maggiorini, Dario; Rossi, Gian Paolo

    2011-01-01

    This paper shows that the deployment of an opportunistic network on any public transportation system leads to obtain a scalable and efficient urban communication platform. We use the term Bus Switched Networks (BSNs) to indicate this urban backbone that complements the services of 3G networks and enables to meet the application level requirements for a large class of applications by ensuring high delivery ratio and acceptable delays under different conditions of packet load. We sustain these ...

  3. The Designing Bus for Nuclear Safety Class Controller

    International Nuclear Information System (INIS)

    EtherCAT (Ethernet for Control Automation Technology) is based on the IEEE 802.3 standard as one of the communication which is the I/O (Input/Output), sensors and communication function of PLC (Programmable Logic Controller) in industry and factory environment use is increasing. The Nuclear Safety Class Controller implemented by the EtherCAT applied bus can be shown the improving performance of data transmission in the controller

  4. Damping torque analysis for DC bus implemented damping control

    OpenAIRE

    W. Du; WANG, HAIFENG; Ju, P.; Dunn, R

    2010-01-01

    Damping torque analysis is a well-developed technique for understanding and studying power system oscillations. This paper presents the applications of damping torque analysis for DC bus implemented damping control in power transmission networks in two examples. The first example is the investigation of damping effect of shunt VSC (Voltage Source Converter) based FACTS voltage control, i.e., STATCOM (Static Synchronous Compensator) voltage control. It is shown in the paper that STATCOM voltag...

  5. New Long-Distance Bus Terminals in Germany

    OpenAIRE

    A. Saxinger; M. Nachtsheim

    2014-01-01

    For many decades long-distance busses were never of great importance in Germany. This part of public transport was almost exclusively provided by railway transportation. Legally, railway transportation was protected from competition. Applications for long-distance bus line concessions had no chance of success.However the amendment of the German Federal Passenger Transportation Act to 1st January 2013 liberalized the German market for long-distance busses significantly. Entrepreneurs still nee...

  6. Peptic ulcer among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1990-01-01

    men in the same age group. The incidence of all manifestations of peptic ulcer disease among bus drivers did not differ from the incidence among Danish men. Of occupational and psycho-social factors, wage dissatisfaction and smoking showed statistically significant association with hospital discharge...... with PU in a 6 3/4-year period. Job dissatisfaction, stress symptoms and lack of some social network factors tended to increase the risk of hospital discharge with PU....

  7. BC Transit Fuel Cell Bus Project: Evaluation Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Post, M.

    2014-02-01

    This report evaluates a fuel cell electric bus demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. This evaluation report covers two years of revenue service data on the buses from April 2011 through March 2013.

  8. Improving bus travel times with passive traffic signal coordination

    OpenAIRE

    Estrada Romeu, Miguel Ángel; Trapote Barreira, César; Roca Riu, Mireia; Robusté Antón, Francesc

    2009-01-01

    A simulation optimization model is outlined: traffic signal offsets in intersections are calculated to minimize the travel time of bus users in an urban network. The model considers a passive signal priority system and restricts the maximal incremental delay caused to car users. The simulation tool is able to trace discrete trajectories of both buses and cars in a network. It also evaluates potential perturbations that may cause time variations for average performance. Moreover, the optimizat...

  9. A simplified model for scheduling services on auxiliary bus lines

    OpenAIRE

    Codina Sancho, Esteve; Montero Mercadé, Lídia

    2014-01-01

    In this paper, a mathematical programming based model is described to assist with the schedule of services for a set of auxiliary bus lines that operate alleviating a disruption of the regular transportation system during a given time period. In contrast to other models, considered static, service schedules are set taking into account demand fluctuations that may happen in that time period. Passenger flows are represented with a multi-commodity structure and disseminate throug...

  10. City of Chula Vista hydrogen fuel cell bus demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, B.; Bamberger, B.

    1996-10-01

    Hydrogen as an energy carrier and fuel has potential for various uses including electricity, commercial, residential, transportation, and industrial. It is an energy carrier that can be produced from a variety of primary sources and potentially can accomplish these various uses while significantly reducing pollution by substituting for or reducing the use of fossil fuels. One of the most immediate and potentially viable roles for hydrogen as an energy carrier will be its use as a transportation fuel, especially in densely populated urban areas where automotive emissions contribute significantly to air pollution. The Department of Energy`s commitment to research and development of hydrogen as an alternative fuel, and California`s Zero Emission Vehicle (ZEV) requirements, both provide the impetus and favorable circumstance for demonstrating hydrogen as a transportation fuel on an urban bus system. The purpose of this project is to demonstrate the feasibility of using solid polymer fuel cells in a hydrogen-powered electric drive system for an urban transit bus application. Fuel cell buses use hydrogen fuel and oxygen from the air to produce electrical power with the only byproduct being pure water. Proton Exchange Membrane (PEM) fuel cells are proposed for this project. Current evidence suggests that fuel cells, which rely on hydrogen and a process known as proton exchange to generate their power, appear to have an infinite life span. All exhaust pollution is completely eliminated, resulting in a Zero Emission Vehicle (ZEV). An urban bus system offers the potential for developing a market for the production of hydrogen propulsion technology due to extensive vehicular use in densely populated areas experiencing pollution from numerous sources, and because the central garaging facilities or the bus system facilitates fueling and maintenance functions.

  11. FAKTOR PENYEBAB KERUSAKAN SISTEM MESIN PADA BUS APTB PPD

    OpenAIRE

    Laela Chairani; Yulita Veranda Usman; Nur Yulianti Hidayah

    2015-01-01

    Comfort and safety of public transportation, particularly APTB PPD buses, becomes high expectations for its customers. Those expectations must be supported by the application of a good engine maintenance system. By using the method of Reliability Centered Maintenance may be determined appropriate actions to mitigate damage. From the analysis known the failures of the APTB PPD bus are malfunctions of the fuel engine, power transfer systems, difficulty to move the gear lever, and lubricating sy...

  12. Oxidative and nitrosative stress markers in bus drivers

    Czech Academy of Sciences Publication Activity Database

    Rössner ml., Pavel; Švecová, Vlasta; Milcová, Alena; Lněničková, Zdena; Solanský, I.; Santella, R. M.; Šrám, Radim

    2007-01-01

    Roč. 617, - (2007), s. 23-32. ISSN 0027-5107 R&D Projects: GA MŽP SL/5/160/05; GA AV ČR 1QS500390506 Institutional research plan: CEZ:AV0Z50390512 Keywords : oxidative stress * bus drivers * air pollution Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 4.159, year: 2007

  13. Modelling urban bus transport emissions in Santiago de Cuba

    OpenAIRE

    Warren, James; Avila, Jordi Mirabent

    2010-01-01

    This study described and focuses on the public transport bus system in Santiago de Cuba. Specifically we explore the fuel consumption of the various diesel buses over a three year period from 2007 to 2009 and with a forecast for the forthcoming years. The study considers the potential for fuel savings through a variety of methods which are each described and then modelled. Pollution is also calculated for a variety of scenarios and some suggestions are made in order to lower the overall...

  14. Market brief : the alternative fuels bus market in India

    International Nuclear Information System (INIS)

    In 2003, alternative fuel bus technologies in India were valued at approximately $1.5 billion. There are an estimated 600,000 buses in India, of which 21 per cent are owned by public transit. Bus production is currently 33,000 per year and increasing at 4 per cent annually. The main alternative fuel bus technologies include fuel cells, compressed natural gas (CNG) and liquefied petroleum gas (LPG). Since urban centres in India are among the most polluted in the world, demand for these technologies is strong. India is ranked fifth in the world in terms of vehicles converted to natural gas. In 2003, New Delhi switched from diesel to CNG fuel for buses but has faced challenges because there is no well-developed underground distribution system and refilling times are long due to weak pressure at fueling stations. India's new $28 million fuel cell bus development project aims to place 8 fuel cell powered buses with the Delhi Transport Corporation. This market brief describes the potential for Canadian suppliers to enter into joint ventures to establish local production facilities and transfer technology expertise. It describes the key factors shaping market growth with particular reference to sector reform, and opportunities with actual and planned projects. The competitive environment was also discussed with reference to local capabilities, international competition, Canadian position, and a competitive advantage through Canadian government policies and initiatives. A section of the report on public-sector customers listed the companies that buy alternative fuel buses in India. Considerations for market-entry in India were also outlined

  15. An Electric Bus with a Battery Exchange System

    OpenAIRE

    Jeongyong Kim; Inho Song; Woongchul Choi

    2015-01-01

    As part of the ongoing effort to be independent of petroleum resources and to be free from pollutant emission issues, various electric vehicles have been developed and tested through their integration with real world systems. In the current paper, yet another application specific EV for public transportation, an electric bus, is introduced and explained with results from the pilot test program which was carried out under real traffic conditions. The main feature of the current system is a bat...

  16. Bus Lanes with Intermittent Priority: Screening Formulae and an Evaluation

    OpenAIRE

    Eichler, Michael; Daganzo, Carlos F.

    2005-01-01

    This paper evaluates strategies for operating buses on signal-controlled arterials using special lanes that are made intermittently available to general traffic. The advantage of special bus lanes, intermittent or dedicated, is that they free buses from traffic interference; the disadvantage is that they disrupt traffic. We find that intermittent lanes, unlike dedicated ones, do not significantly reduce street capacity. Intermittence, however, increases the average traffic density at which th...

  17. Hardware Engines for Bus Encryption: a Survey of Existing Techniques

    OpenAIRE

    Elbaz, Reouven; Torres, Lionel; Sassatelli, Gilles; GUILLEMIN, Pierre; Anguille, Claude; Bardouillet, Michel; Buatois, Christian; Rigaud, Jean-Baptiste

    2005-01-01

    The widening spectrum of applications and services provided by portable and embedded devices bring a new dimension of concerns in security. Most of those embedded systems (pay-TV, PDAs, mobile phones, etc...) make use of external memory. As a result, the main problem is that data and instructions are constantly exchanged between memory (RAM) and CPU in clear form on the bus. This memory may contain confidential data like commercial software or private contents, which either the end-user or th...

  18. Irradiation test of bar code label

    International Nuclear Information System (INIS)

    The irradiation test of bar code label tagged on radioactive waste container was done to determine the effect of radiation. Low and medium radioactive waste is that below total activity of 4,000Bq/g according to the Korean nuclear law. The irradiation amount to radiate bar code label tagged on radioactive waste container was calculated by MCNP-4b computer code. The nuclide such as Co-60 and Cs-137 was assumed to contribute 50 % of total activity. Real irradiation amount for bar code label was finally calculated by the dimensions of the container and the bar code label. The identification of post and the physical deflection of irradiated bar code label was tested by the bar code reader. The coated bar code label was suitable to use on low and medium radioactive waste container

  19. Proximity-dependent and proximity-independent trans-splicing in mammalian cells

    OpenAIRE

    Viles, Kristi D.; Sullenger, Bruce A

    2008-01-01

    Most human pre-mRNAs are cis-spliced, removing introns and joining flanking exons of the same RNA molecule. However, splicing of exons present on separate pre-mRNA molecules can also occur. This trans-splicing reaction can be exploited by pre-trans-splicing molecules (PTMs), which are incapable of cis-splicing. PTM-mediated trans-splicing has been utilized to repair mutant RNAs as a novel approach to gene therapy. Herein we explore how the site of PTM expression influences trans-splicing acti...

  20. Alternative splicing of SMPD1 in human sepsis.

    Directory of Open Access Journals (Sweden)

    Marcel Kramer

    Full Text Available Acid sphingomyelinase (ASM or sphingomyelin phosphodiesterase, SMPD activity engages a critical role for regulation of immune response and development of organ failure in critically ill patients. Beside genetic variation in the human gene encoding ASM (SMPD1, alternative splicing of the mRNA is involved in regulation of enzymatic activity. Here we show that the patterns of alternatively spliced SMPD1 transcripts are significantly different in patients with systemic inflammatory response syndrome and severe sepsis/septic shock compared to control subjects allowing discrimination of respective disease entity. The different splicing patterns might contribute to the better understanding of the pathophysiology of human sepsis.

  1. Perspective Application of Passive Optical Network with Optimized Bus Topology

    Directory of Open Access Journals (Sweden)

    P. Lafata

    2012-06-01

    Full Text Available Passive optical networks (PONs represent a promising solution for modern access telecommunication networks.These networks are able to meet the increasing demands on transmission rate for demanding multimedia services,while they can offer typical shared transmission speed of 1.25 or 2.5 Gbps. The major role in deploying opticaldistribution networks ODNs plays the maximum attenuable loss, which is caused mainly by passive optical splitters.This paper proposes an innovative application of passive optical networks with optimized bus topology especially forlocal backbone data networks. Due to using only passive components, it is necessary to optimize certain parameters,especially an overall attenuation balance. Considering the possibility of such optimization, the passive optical networkwith optimized bus topology provides several interesting opportunities for specific applications. This paper will presentselected aspects of passive optical networks and splitters with asymmetric splitting ratio. The essential part is focusedon the practical demonstration of their use to optimize the passive optical network with bus topology, which acts as alocal backbone network for structured cabling systems, and for local data networks in large buildings.

  2. The AI Bus architecture for distributed knowledge-based systems

    Science.gov (United States)

    Schultz, Roger D.; Stobie, Iain

    1991-01-01

    The AI Bus architecture is layered, distributed object oriented framework developed to support the requirements of advanced technology programs for an order of magnitude improvement in software costs. The consequent need for highly autonomous computer systems, adaptable to new technology advances over a long lifespan, led to the design of an open architecture and toolbox for building large scale, robust, production quality systems. The AI Bus accommodates a mix of knowledge based and conventional components, running on heterogeneous, distributed real world and testbed environment. The concepts and design is described of the AI Bus architecture and its current implementation status as a Unix C++ library or reusable objects. Each high level semiautonomous agent process consists of a number of knowledge sources together with interagent communication mechanisms based on shared blackboards and message passing acquaintances. Standard interfaces and protocols are followed for combining and validating subsystems. Dynamic probes or demons provide an event driven means for providing active objects with shared access to resources, and each other, while not violating their security.

  3. The Data Acquisition System Based on PMC Bus

    CERN Document Server

    Igarashi, Y; Higuchi, T; Ikeno, M; Inoue, E; Itoh, R; Kodama, H; Murakami, T; Nagasaka, Y; Nakao, M; Nakayoshi, K; Saitoh, M; Shimazaki, S; Suzuki, S Y; Tanaka, M; Tauchi, K; Varner, G; Yamauchi, M; Yasu, Y; Katayama, T; Watanabe, K; Ishizuka, M; Onozawa, S

    2003-01-01

    High energy physics experiments in KEK/Japan rush into over KHz trigger stage. Thus, we need a successor of the data acquisition(DAQ) system that replaces the CAMAC or FASTBUS systems. To meet these needs, we have developed a DAQ system which includes a crate, base-board modules, daughter cards for front-end A/D or T/D conversion, and back-end communication cards for data transfer and timing control. The size of the crate is for the 9U Euro-cards with the standard VME32 bus and extension connectors for power supply. The base-board comprises of a local bus with the sequencer connected to the front-end daughter cards via event buffering FIFOs, and the standard PMC (PCI mezzanine card) bus to be set a PMC processor unit to reduce data size from the front-end daughter cards. A data transfer module, which is connected to the event building system, and a trigger control unit, which communicates with the central timing controller are installed on the back-end communication card connected to the rear end of the base-...

  4. Safety evaluation of a hydrogen fueled transit bus

    Energy Technology Data Exchange (ETDEWEB)

    Coutts, D.A.; Thomas, J.K.; Hovis, G.L.; Wu, T.T. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1997-12-31

    Hydrogen fueled vehicle demonstration projects must satisfy management and regulator safety expectations. This is often accomplished using hazard and safety analyses. Such an analysis has been completed to evaluate the safety of the H2Fuel bus to be operated in Augusta, Georgia. The evaluation methods and criteria used reflect the Department of Energy`s graded approach for qualifying and documenting nuclear and chemical facility safety. The work focused on the storage and distribution of hydrogen as the bus motor fuel with emphases on the technical and operational aspects of using metal hydride beds to store hydrogen. The safety evaluation demonstrated that the operation of the H2Fuel bus represents a moderate risk. This is the same risk level determined for operation of conventionally powered transit buses in the United States. By the same criteria, private passenger automobile travel in the United States is considered a high risk. The evaluation also identified several design and operational modifications that resulted in improved safety, operability, and reliability. The hazard assessment methodology used in this project has widespread applicability to other innovative operations and systems, and the techniques can serve as a template for other similar projects.

  5. An Electric Bus with a Battery Exchange System

    Directory of Open Access Journals (Sweden)

    Jeongyong Kim

    2015-07-01

    Full Text Available As part of the ongoing effort to be independent of petroleum resources and to be free from pollutant emission issues, various electric vehicles have been developed and tested through their integration with real world systems. In the current paper, yet another application specific EV for public transportation, an electric bus, is introduced and explained with results from the pilot test program which was carried out under real traffic conditions. The main feature of the current system is a battery exchanging mechanism mounted on the roof of the bus. The current configuration certainly requires an externally fabricated battery exchanging robot system that would complement the electric bus for a fully automated battery exchanging process. The major advantage of the current system is the quick re-charging of the electric energy through the physical battery exchange and the possible utilization of the battery exchange station as a mini scale energy storage system for grid system peak power shaving. With the total system solution approach for the public transportation system, it is fully expected to create outstanding business opportunities in number of areas such as battery suppliers, battery exchanging station management, battery leasing and many more.

  6. Common Traffic Violations of Bus Drivers in Urban China: An Observational Study.

    Directory of Open Access Journals (Sweden)

    Qiqi Wang

    Full Text Available To report common traffic violations in bus drivers and the factors that influence those violations in urban China.We conducted an observational study to record three types of traffic violations among bus drivers in Changsha City, China: illegal stopping at bus stations, violating traffic light signals, and distracted driving. The behaviors of bus drivers on 32 routes (20% of bus routes in the city were observed. A two-level Poisson regression examined factors that predicted bus driver violations.The incidence of illegal stopping at bus stations was 20.2%. Illegal stopping was less frequent on weekends, sunny days, and at stations with cameras, with adjusted incidence rate ratios (IRRs of 0.81, 0.65 and 0.89, respectively. The incidence of violating traffic light signals was 2.2%, and was lower on cloudy than sunny days (adjusted IRR: 0.60. The incidence of distracted driving was 3.3%. The incidence of distracted driving was less common on cloudy days, rainy or snowy days, and foggy/windy/dusty days compared to sunny days, with adjusted IRRs of 0.54, 0.55 and 0.07, respectively.Traffic violations are common in bus drivers in urban China and they are associated with the date, weather, and presence of traffic cameras at bus station. Further studies are recommended to understand the behavioral mechanisms that may explain bus driver violations and to develop feasible prevention measures.

  7. Capacity models on expressway near a bus bay stop with an access

    Institute of Scientific and Technical Information of China (English)

    张洪宾; 孙小端; 贺玉龙; 宇仁德

    2015-01-01

    To determinate the expressway capacity near a bus bay stop with an access, capacity models on the expressway near a bus stop with an access were developed on the basis of gap acceptance theory and queuing theory. Depending on a bus stop position to an entrance or an exit ramp, the capacity models were developed for four cases. Bus bay stops with overflow and bus bay stops without overflow were considered. A comparison of simulation experiment and model calculation was carried out. Results show that the suggested models have high accuracy and reliability, at bus arrival rate below 60 vehicles per hour (veh/h) or vehicle volumes at the entrance and the exit below 200 passenger cars units per hour (pcu/h), and there are no significant difference in the capacities for four cases. When bus arrival rate is above 240 veh/h, the capacities of all four cases will decline rapidly. With berth number increasing, the increasing of the capacities is no obvious for four cases. As the bus arrival rate and vehicle volumes at the entrance and the exit increase, bus stops located downstream of an entrance and upstream of an exit have a remarkably effect on the capacities. The latter case is much heavier than the former. Those results can be used to traffic design and optimization on urban expressway near a bus stop with an access.

  8. An Optimization Model for the Selection of Bus-Only Lanes in a City.

    Science.gov (United States)

    Chen, Qun

    2015-01-01

    The planning of urban bus-only lane networks is an important measure to improve bus service and bus priority. To determine the effective arrangement of bus-only lanes, a bi-level programming model for urban bus lane layout is developed in this study that considers accessibility and budget constraints. The goal of the upper-level model is to minimize the total travel time, and the lower-level model is a capacity-constrained traffic assignment model that describes the passenger flow assignment on bus lines, in which the priority sequence of the transfer times is reflected in the passengers' route-choice behaviors. Using the proposed bi-level programming model, optimal bus lines are selected from a set of candidate bus lines; thus, the corresponding bus lane network on which the selected bus lines run is determined. The solution method using a genetic algorithm in the bi-level programming model is developed, and two numerical examples are investigated to demonstrate the efficacy of the proposed model. PMID:26214001

  9. Development and validation of a self-report measure of bus driver behaviour.

    Science.gov (United States)

    Dorn, Lisa; Stephen, Lucy; af Wåhlberg, Anders; Gandolfi, Julie

    2010-12-01

    There are likely to be individual differences in bus driver behaviour when adhering to strict schedules under time pressure. A reliable and valid assessment of these individual differences would be useful for bus companies keen to mitigate risk of crash involvement. This paper reports on three studies to develop and validate a self-report measure of bus driver behaviour. For study 1, two principal components analyses of a pilot questionnaire revealed six components describing bus driver behaviour and four bus driver coping components. In study 2, test-retest reliability of the components were tested in a sub-sample and found to be adequate. Further, the 10 components were used to predict bus crash involvement at three levels of culpability with consistently significant associations found for two components. For study 3, avoidance coping was consistently associated with celeration variables in a bus simulator, especially for a time-pressured drive. STATEMENT OF RELEVANCE: The instrument can be used by bus companies for driver stress and fatigue management training to identify at-risk bus driver behaviour. Training to reduce the tendency to engage in avoidance coping strategies, improve evaluative coping strategies and hazard monitoring when under stress may improve bus driver safety. PMID:21108079

  10. BUS BAY PERFORMANCE AND ITS INFLUENCE ON THE CAPACITY OF ROAD NETWORK IN DAR ES SALAAM

    Directory of Open Access Journals (Sweden)

    Nurdin K. Mushule

    2012-01-01

    Full Text Available One of major problems facing motorists in Dar es Salaam city today is congestion. Bus bays have a significant influence on the capacity of a roadway because they interfere with passing vehicles primarily when buses maneuver to pull into and out of bus bays. Bus bay stops will also interfere with vehicles movement if bus demand exceeds the bus bay capacity resulting in some buses waiting in the travel lane until the buses occupying the bay exit the bay. This paper presents the results of a study which was carried out to evaluate the bus bay performance and its influence on the capacity of the roadway network in the city of Dar es Salaam. The case study area covered 11 bus stops along Morogoro road from Ubungo to Magomeni Mapipa. Capacity of bus bays was studied using procedure outlined in the Transit Capacity and Quality of Service Manual of 2003. This enabled the researcher to determine parameters such as dwell times and clearance times which are major determinants of bus stop capacity. The results indicate that only 18% of the bus bay stops studied did not have adequate capacity to cater for the available demand. 9% did not have adequate capacity during peak hours but the capacity was adequate during off-peak hours. The remaining 73% of bus bay stops possess adequate capacity all the time. Although most bus bay stops studied possess adequate capacity, severe congestion was observed at these locations. This is due to erratic behavior of bus drivers who do not utilize the provided space for them to drop off and pick up passengers. Clearly, this is an area that requires more strict enforcement in order to ease the congestion problem in the city by operating the existing capacity more efficiently."

  11. Atomic simulations of (10 1-bar 2), (10 1-bar 1) twinning and (10 1-bar 2) detwinning in magnesium

    International Nuclear Information System (INIS)

    (10 1-bar 2) and (10 1-bar 1) twinning and (10 1-bar 2) detwinning for Mg were investigated from the viewpoint of mobility of twinning dislocations and atomic shuffling. First-principles calculations suggested that the twinning dislocations glide more readily for the (10 1-bar 1) twinning than for the (10 1-bar 2) twinning. However, this conflicts with the experimental fact of easier (10 1-bar 2) twin formation. On the other hand, molecular dynamics simulations showed that the atomic shuffling was more activated for the (10 1-bar 2) twinning than for the (10 1-bar 1) twinning, which corresponds to the experimental fact. Therefore, it is suggested that the rate-controlling process for the twin formation is the atomic shuffling. Moreover, the calculations and simulations showed that the twinning dislocations glide more readily for the (10 1-bar 2) detwinning than for the (10 1-bar 2) twinning, whereas the atomic shuffling is less activated for the detwinning, suggesting that the detwinning occurs easily but is unstable, resulting in easy repetition of twinning–detwinning. (paper)

  12. Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function

    OpenAIRE

    Glauser, Dominique A; Johnson, Brandon E.; Aldrich, Richard W; Goodman, Miriam B.

    2012-01-01

    Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded by the Caenorhabditis elegans slo-1 gene, containing three alternate splice sites. Using conditional probability-based models, we show that splicing events are coordinated acros...

  13. EVOLUTION OF SR PROTEIN AND HnRNP SPLICING REGULATORY FACTORS

    OpenAIRE

    Busch, A.; Hertel, KJ

    2011-01-01

    The splicing of pre-mRNAs is an essential step of gene expression in eukaryotes. Introns are removed from split genes through the activities of the spliceosome, a large ribonuclear machine that is conserved throughout the eukaryotic lineage. While unicellular eukaryotes are characterized by less complex splicing, pre-mRNA splicing of multicellular organisms is often associated with extensive alternative splicing that significantly enriches their proteome. The alternative selection of splice s...

  14. Editing efficiency of a Drosophila gene correlates with a distant splice site selection

    OpenAIRE

    AGRAWAL, RITESH; Stormo, Gary D.

    2005-01-01

    RNA editing and alternative splicing are two processes that increase protein diversity. The relationship between the two processes is not well understood. There are a few examples of correlations between editing and alternative splicing, but these are all nearby effects. A search for alternative splicing among 16 edited genes in Drosophila reveals two novel instances of alternative splicing. In one example where alternative splicing occurs downstream of editing, a strong correlation between e...

  15. Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila

    OpenAIRE

    Khodor, Yevgenia L.; Rodriguez, Joseph; Abruzzi, Katharine C.; Tang, Chih-Hang Anthony; Marr, Michael T.; Rosbash, Michael

    2011-01-01

    Cotranscriptional splicing, in which mRNA is spliced as it is being transcribed, is thought to be necessary for proper gene regulation of many genes in eukaryotic cells. While studies have shown that splicing takes place cotranscriptionally in yeast, in higher eukaryotes, where genes contain multiple introns with widespread alternative splicing, the question of whether cotranscriptional splicing is a general phenomenon remains. Khodor et al. investigated what fractions of genes are cotranscri...

  16. Epstein-Barr Virus SM Protein Functions as an Alternative Splicing Factor ▿

    OpenAIRE

    Verma, Dinesh; Swaminathan, Sankar

    2008-01-01

    Alternative splicing of RNA increases the coding potential of the genome and allows for additional regulatory control over gene expression. The full extent of alternative splicing remains to be defined but is likely to significantly expand the size of the human transcriptome. There are several examples of mammalian viruses regulating viral splicing or inhibiting cellular splicing in order to facilitate viral replication. Here, we describe a viral protein that induces alternative splicing of a...

  17. Correction of tau mis-splicing caused by FTDP-17 MAPT mutations by spliceosome-mediated RNA trans-splicing

    OpenAIRE

    Rodriguez-Martin, Teresa; Anthony, Karen; Garcia-Blanco, Mariano A.; Mansfield, S. Gary; Anderton, Brian H.; Gallo, Jean-Marc

    2009-01-01

    Frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17) is caused by mutations in the MAPT gene, encoding the tau protein that accumulates in intraneuronal lesions in a number of neurodegenerative diseases. Several FTDP-17 mutations affect alternative splicing and result in excess exon 10 (E10) inclusion in tau mRNA. RNA reprogramming using spliceosome-mediated RNA trans-splicing (SMaRT) could be a method of choice to correct aberrant E10 splicing resulting from FTDP-17 mu...

  18. Life cycle cost analysis of a car, a city bus and an intercity bus powertrain for year 2005 and 2020

    International Nuclear Information System (INIS)

    The international economy, in the beginning of the 20th century, is characterized by uncertainty about the supply and the price of oil. Together with the fast decrease of electrical propulsion component prices, it becomes more and more cost effective to develop vehicles with alternative powertrains. This paper focuses on two questions: Are alternative powertrains especially cost effective for specific applications?; How does an increased fossil fuel price influences the choose of powertrain? To assess these questions, a computer tool named THEPS, developed in a Ph.D. project, is used. Three applications and three scenarios are analysed. The applications, a car, a city bus and an intercity bus, are vehicles all assumed to operate in Sweden. One scenario represents year 2005, the other two year 2020. The two future scenarios are characterized by different fossil fuel prices. The study, presented in the paper, indicates that alternative powertrains can be competitive from a cost perspective, in some applications, already in year 2005. It is for example cost effective to equip a city bus, running in countries with a high fuel price, with a hybrid powertrain. The study also indicates that pure electric, hybrid and/or fuel cell cars will probably be a more cost effective choice than conventional cars in year 2020. Another indication is that it will not be clear which powertrain concept to choose. The reason is that many cost effective powertrain concepts will be offered. The best choice will depend on the application

  19. First observation of B-bars0→Ds2*+Xμ-ν-bar decays

    International Nuclear Information System (INIS)

    Using data collected with the LHCb detector in proton-proton collisions at a centre-of-mass energy of 7 TeV, the semileptonic decays B-bars0→Ds+Xμ-ν-bar and B-bars0→D0K+Xμ-ν-bar are detected. Two structures are observed in the D0K+ mass spectrum at masses consistent with the known Ds1(2536)+ and Ds2*(2573)+ mesons. The measured branching fractions relative to the total B-bars0 semileptonic rate are B(B-bars0→Ds2*+Xμ-ν-bar)/B(B-bars0→Xμ-ν-bar)=(3.3±1.0±0.4)%, and B(B-bars0→Ds1+Xμ-ν-bar)/B(B-bars0→Xμ-ν-bar)=(5.4±1.2±0.5)%, where the first uncertainty is statistical and the second is systematic. This is the first observation of the Ds2*+ state in B-bars0 decays; we also measure its mass and width.

  20. Network of evolutionary processors with splicing rules and permitting context.

    Science.gov (United States)

    Choudhary, Ashish; Krithivasan, Kamala

    2007-02-01

    In this paper we consider networks of evolutionary processors with splicing rules and permitting context (NEPPS) as language generating and computational devices. Such a network consists of several processors placed on the nodes of a virtual graph and are able to perform splicing (which is a biologically motivated operation) on the words present in that node, according to the splicing rules present there. Before applying the splicing operation on words, we check for the presence of certain symbols (permitting context) in the strings on which the rule is applied. Each node is associated with an input and output filter. When the filters are based on random context conditions, one gets the computational power of Turing machines with networks of size two. We also show how these networks can be used to solve NP-complete problems in linear time. PMID:17045388

  1. Synaptic signaling and aberrant RNA splicing in autism spectrum disorders

    Directory of Open Access Journals (Sweden)

    Ryan M Smith

    2011-01-01

    Full Text Available Interactions between presynaptic and postsynaptic cellular adhesion molecules drive synapse maturation during development. These trans-synaptic interactions are regulated by alternative splicing of cellular adhesion molecule RNAs, which ultimately determines neurotransmitter phenotype. The diverse assortment of RNAs produced by alternative splicing generates countless protein isoforms necessary for guiding specialized cell-to-cell connectivity. Failure to generate the appropriate synaptic adhesion proteins is associated with disrupted glutamatergic and gamma-aminobutyric acid signaling, resulting in loss of activity-dependent neuronal plasticity, and risk for developmental disorders, including autism. While the majority of genetic mutations currently linked to autism are rare variants that change the protein coding sequence of synaptic candidate genes, regulatory polymorphisms affecting constitutive and alternative splicing have emerged as risk factors in numerous other diseases, accounting for an estimated 40-60% of general disease risk. Here, we review the relationship between aberrant RNA splicing of synapse-related genes and autism spectrum disorders.

  2. Designing oligo libraries taking alternative splicing into account

    Science.gov (United States)

    Shoshan, Avi; Grebinskiy, Vladimir; Magen, Avner; Scolnicov, Ariel; Fink, Eyal; Lehavi, David; Wasserman, Alon

    2001-06-01

    We have designed sequences for DNA microarrays and oligo libraries, taking alternative splicing into account. Alternative splicing is a common phenomenon, occurring in more than 25% of the human genes. In many cases, different splice variants have different functions, are expressed in different tissues or may indicate different stages of disease. When designing sequences for DNA microarrays or oligo libraries, it is very important to take into account the sequence information of all the mRNA transcripts. Therefore, when a gene has more than one transcript (as a result of alternative splicing, alternative promoter sites or alternative poly-adenylation sites), it is very important to take all of them into account in the design. We have used the LEADS transcriptome prediction system to cluster and assemble the human sequences in GenBank and design optimal oligonucleotides for all the human genes with a known mRNA sequence based on the LEADS predictions.

  3. Standard Bus System on Accelerator and the Possibility of Its Application in P3TM

    International Nuclear Information System (INIS)

    Usually accelerator in the research and applied laboratory already used standard bus system on their control systems. In the beginning several laboratories uses CAMAC system and then gradually use to VME or VXI bus such as at Continuous Electron Beam Accelerator Facility (CEBAF) in VA USA, National Laboratory for High Energy Physics (KEK) in Tsukuba Japan, and National Synchrotron Light Source (NSLS) in Brookhaven National Laboratory (BNL) Upton, NY, USA. On China since pre-requirement it has been decided to use VME bus. EPICS program have been developed in the world for physics experiment, VME bus control system. P3TM needs to study VME bus and its availability on the market, also to make collaboration with EPICS user and try to obtain it if standard bus for control system is to be considered. (author)

  4. Identification of voltage stability condition of a power system using measurements of bus variables

    Directory of Open Access Journals (Sweden)

    Durlav Hazarika

    2014-12-01

    Full Text Available Several online methods were proposed for investigating the voltage stability condition of an interconnected power system using the measurements of voltage and current phasors at a bus. For this purpose, phasor measurement units (PMUs are used. A PMU is a device which measures the electrical waves on an electrical network, using a common time source (reference bus for synchronisation. This study proposes a method for online monitoring of voltage stability condition of a power system using measurements of bus variables namely – (i real power, (ii reactive power and (iii bus voltage magnitude at a bus. The measurements of real power, reactive power and bus voltage magnitude could be extracted/captured from a smart energy meter. The financial involvement for implementation of the proposed method would significantly lower compared with the PMU-based method.

  5. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Fourth Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2013-01-01

    SunLine Transit Agency, which provides public transit services to the Coachella Valley area of California, has demonstrated hydrogen and fuel cell bus technologies for more than 10 years. In May 2010, SunLine began demonstrating the advanced technology (AT) fuel cell bus with a hybrid electric propulsion system, fuel cell power system, and lithium-based hybrid batteries. This report describes operations at SunLine for the AT fuel cell bus and five compressed natural gas buses. The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) is working with SunLine to evaluate the bus in real-world service to document the results and help determine the progress toward technology readiness. NREL has previously published three reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from February 2012 through November 2012.

  6. Complex motion of a shuttle bus between two terminals with periodic inflows

    Science.gov (United States)

    Nagatani, Takashi

    2016-05-01

    We investigate the dynamic behavior of a shuttle bus serving repeatedly between two terminals when passengers come periodically at two terminals A and B. The period and phase of the periodic inflow of passengers at terminal A are different from those at terminal B. The dynamic behavior of the bus is highly affected by the difference between two periods and the phase difference. The bus schedule is closely related to the dynamics. We present the extended circle map model for the dynamics of the shuttle bus. The motion of the shuttle bus depends highly on the inflow periods, the period difference, the phase difference, and the loading parameter. The shuttle bus displays the periodic, quasi-periodic, and chaotic motions. The quasi-periodic motion changes to a chaotic motion by the difference between two periods.

  7. Bitmap-Wise Wireless M-Bus Coordination for Sustainable Real Time Energy Management

    Directory of Open Access Journals (Sweden)

    Kwang-il Hwang

    2014-07-01

    Full Text Available Even though WM-Bus is being considered to be the most promising network protocol for smart metering, it is not suitable for a sustainable real-time home energy management system (HEMS, which requires higher reliability and longer lifetime despite real time bi-directional communications. Therefore, in this paper we propose a Bitmap-wise WM-Bus (BWM-Bus, coping well with sustainable real-time HEMS. In particular, the proposed scheme addresses the several problems in WM-Bus for HEMS by introducing novel functions: asynchronous meter trigger, adaptive slot scheduling, and bitmap-wise retransmission request. Through experiments, we demonstrate that BWM-Bus guarantees higher data success ratio with lower data aggregation time, as well as longer lifetime than WM-Bus standard.

  8. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    OpenAIRE

    Zodwa Dlamini; Tshidino, Shonisani C.; Rodney Hull

    2015-01-01

    Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 f...

  9. Alternative Splicing of Type II Procollagen: IIB or not IIB?

    OpenAIRE

    McAlinden, Audrey

    2014-01-01

    Over two decades ago, two isoforms of the type II procollagen gene (COL2A1) were discovered. These isoforms, named IIA and IIB, are generated in a developmentally-regulated manner by alternative splicing of exon 2. Chondroprogenitor cells synthesize predominantly IIA isoforms (containing exon 2) while differentiated chondrocytes produce mainly IIB transcripts (devoid of exon 2). Importantly, this IIA-to-IIB alternative splicing switch occurs only during chondrogenesis. More recently, two othe...

  10. Diagnosis of trypanosomatid infections : targeting the spliced leader RNA

    OpenAIRE

    Gonzalez-Andrade, P.; M. Camara; Ilboudo, H.; Buheton, B.; Jamonneau, Vincent; Deborggraeve, S

    2014-01-01

    Trypanosomatids transcribe their genes in Large polycistronic clusters that are further processed into mature mRNA molecules by trans-splicing. During this maturation process, a conserved spliced leader RNA (SL-RNA) sequence of 39 bp is physically linked to the 5' end of the pre-mRNA molecules. Trypanosomatid infections cause a series of devastating diseases in man (sleeping sickness, leishmaniasis, Chagas disease) and animals (nagana, surra, dourine). Here, we investigated the SL-RNA molecul...

  11. RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing

    OpenAIRE

    Guo, Wei; Schafer, Sebastian; Greaser, Marion L.; Radke, Michael H.; Liss, Martin; Govindarajan, Thirupugal; Maatz, Henrike; Schulz, Herbert; Li, Shijun; Parrish, Amanda M.; Dauksaite, Vita; Vakeel, Padmanabhan; Klaassen, Sabine; Gerull, Brenda; Thierfelder, Ludwig

    2012-01-01

    Alternative splicing plays a major role in the adaptation of cardiac function exemplified by the isoform switch of titin, which adjusts ventricular filling. We previously identified a rat strain deficient in titin splicing. Using genetic mapping, we found a loss-of-function mutation in RBM20 as the underlying cause for the pathological titin isoform expression. Mutations in human RBM20 have previously been shown to cause dilated cardiomyopathy. We showed that the phenotype of Rbm20 deficient ...

  12. Functional roles of alternative splicing factors in human disease

    OpenAIRE

    Cieply, Benjamin; Carstens, Russ P.

    2015-01-01

    Alternative splicing (AS) is an important mechanism used to generate greater transcriptomic and proteomic diversity from a finite genome. Nearly all human gene transcripts are alternatively spliced and can produce protein isoforms with divergent and even antagonistic properties that impact cell functions. Many AS events are tightly regulated in a cell-type or tissue-specific manner, and at different developmental stages. AS is regulated by RNA-binding proteins, including cell- or tissue-speci...

  13. Altered PLP1 splicing causes hypomyelination of early myelinating structures

    OpenAIRE

    Kevelam, Sietske H.; Taube, Jennifer R.; van Spaendonk, Rosalina M. L.; Bertini, Enrico; Sperle, Karen; Tarnopolsky, Mark; Tonduti, Davide; Valente, Enza Maria; Travaglini, Lorena; Sistermans, Erik A.; Bernard, Geneviève; Catsman-Berrevoets, Coriene E.; van Karnebeek, Clara D M; Østergaard, John R.; Friederich, Richard L

    2015-01-01

    Objective The objective of this study was to investigate the genetic etiology of the X-linked disorder “Hypomyelination of Early Myelinating Structures” (HEMS). Methods We included 16 patients from 10 families diagnosed with HEMS by brain MRI criteria. Exome sequencing was used to search for causal mutations. In silico analysis of effects of the mutations on splicing and RNA folding was performed. In vitro gene splicing was examined in RNA from patients’ fibroblasts and an immortalized immatu...

  14. A Unique, Consistent Identifier for Alternatively Spliced Transcript Variants

    OpenAIRE

    Riva, Alberto; Pesole, Graziano

    2009-01-01

    Background As research into alternative splicing reveals the fundamental importance of this phenomenon in the genome expression of higher organisms, there is an increasing need for a standardized, consistent and unique identifier for alternatively spliced isoforms. Such an identifier would be useful to eliminate ambiguities in references to gene isoforms, and would allow for the reliable comparison of isoforms from different sources (e.g., known genes vs. computational predictions). Commonly ...

  15. Interrogation of alternative splicing events in duplicated genes during evolution

    OpenAIRE

    Chen Ting-Wen; Wu Timothy H; Ng Wailap V; Lin Wen-Chang

    2011-01-01

    Abstract Background Gene duplication provides resources for developing novel genes and new functions while retaining the original functions. In addition, alternative splicing could increase the complexity of expression at the transcriptome and proteome level without increasing the number of gene copy in the genome. Duplication and alternative splicing are thought to work together to provide the diverse functions or expression patterns for eukaryotes. Previously, it was believed that duplicati...

  16. Purifying Selection on Exonic Splice Enhancers in Intronless Genes.

    Science.gov (United States)

    Savisaar, Rosina; Hurst, Laurence D

    2016-06-01

    Exonic splice enhancers (ESEs) are short nucleotide motifs, enriched near exon ends, that enhance the recognition of the splice site and thus promote splicing. Are intronless genes under selection to avoid these motifs so as not to attract the splicing machinery to an mRNA that should not be spliced, thereby preventing the production of an aberrant transcript? Consistent with this possibility, we find that ESEs in putative recent retrocopies are at a higher density and evolving faster than those in other intronless genes, suggesting that they are being lost. Moreover, intronless genes are less dense in putative ESEs than intron-containing ones. However, this latter difference is likely due to the skewed base composition of intronless sequences, a skew that is in line with the general GC richness of few exon genes. Indeed, after controlling for such biases, we find that both intronless and intron-containing genes are denser in ESEs than expected by chance. Importantly, nucleotide-controlled analysis of evolutionary rates at synonymous sites in ESEs indicates that the ESEs in intronless genes are under purifying selection in both human and mouse. We conclude that on the loss of introns, some but not all, ESE motifs are lost, the remainder having functions beyond a role in splice promotion. These results have implications for the design of intronless transgenes and for understanding the causes of selection on synonymous sites. PMID:26802218

  17. The functional modulation of epigenetic regulators by alternative splicing

    Directory of Open Access Journals (Sweden)

    Martínez-Balbás Marian

    2007-07-01

    Full Text Available Abstract Background Epigenetic regulators (histone acetyltransferases, methyltransferases, chromatin-remodelling enzymes, etc play a fundamental role in the control of gene expression by modifying the local state of chromatin. However, due to their recent discovery, little is yet known about their own regulation. This paper addresses this point, focusing on alternative splicing regulation, a mechanism already known to play an important role in other protein families, e.g. transcription factors, membrane receptors, etc. Results To this end, we compiled the data available on the presence/absence of alternative splicing for a set of 160 different epigenetic regulators, taking advantage of the relatively large amount of unexplored data on alternative splicing available in public databases. We found that 49 % (70 % in human of these genes express more than one transcript. We then studied their alternative splicing patterns, focusing on those changes affecting the enzyme's domain composition. In general, we found that these sequence changes correspond to different mechanisms, either repressing the enzyme's function (e.g. by creating dominant-negative inhibitors of the functional isoform or creating isoforms with new functions. Conclusion We conclude that alternative splicing of epigenetic regulators can be an important tool for the function modulation of these enzymes. Considering that the latter control the transcriptional state of large sets of genes, we propose that epigenetic regulation of gene expression is itself strongly regulated by alternative splicing.

  18. SF3B1 Association with Chromatin Determines Splicing Outcomes

    Directory of Open Access Journals (Sweden)

    Nir Kfir

    2015-04-01

    Full Text Available Much remains unknown concerning the mechanism by which the splicing machinery pinpoints short exons within intronic sequences and how splicing factors are directed to their pre-mRNA targets. One probable explanation lies in differences in chromatin organization between exons and introns. Proteomic, co-immunoprecipitation, and sedimentation analyses described here indicate that SF3B1, an essential splicing component of the U2 snRNP complex, is strongly associated with nucleosomes. ChIP-seq and RNA-seq analyses reveal that SF3B1 specifically binds nucleosomes located at exonic positions. SF3B1 binding is enriched at nucleosomes positioned over short exons flanked by long introns that are also characterized by differential GC content between exons and introns. Disruption of SF3B1 binding to such nucleosomes affects splicing of these exons similarly to SF3B1 knockdown. Our findings suggest that the association of SF3B1 with nucleosomes is functionally important for splice-site recognition and that SF3B1 conveys splicing-relevant information embedded in chromatin structure.

  19. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    Directory of Open Access Journals (Sweden)

    Zodwa Dlamini

    2015-11-01

    Full Text Available Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 family have alternatively spliced isoforms that lack important active domains. These isoforms can play a negative regulatory role by binding to and inhibiting the pro-apoptotic forms. Alternative splicing is observed to be increased in various cardiovascular diseases with the level of alternate transcripts increasing elevated in diseased hearts compared to healthy subjects. In many cases these isoforms appear to be the underlying cause of the disease, while in others they may be induced in response to cardiovascular pathologies. Regardless of this, the detection of alternate splicing events in the heart can serve as useful diagnostic or prognostic tools, while those splicing events that seem to play a causative role in cardiovascular disease make attractive future drug targets.

  20. Tafazzin splice variants and mutations in Barth syndrome.

    Science.gov (United States)

    Kirwin, Susan M; Manolakos, Athena; Barnett, Sarah Swain; Gonzalez, Iris L

    2014-01-01

    Barth syndrome is caused by mutations in the TAZ (tafazzin) gene on human chromosome Xq28. The human tafazzin gene produces four major mRNA splice variants; two of which have been shown to be functional (TAZ lacking exon 5 and full-length) in complementation studies with yeast and Drosophila. This study characterizes the multiple alternative splice variants of TAZ mRNA and their proportions in blood samples from a cohort of individuals with Barth syndrome (BTHS). Because it has been reported that collection and processing methods can affect the expression of various genes, we tested and chose a stabilizing medium for collecting, shipping and processing of the blood samples of these individuals. In both healthy controls and in BTHS individuals, we found a greater variety of alternatively spliced forms than previously described, with a sizeable proportion of minor splice variants besides the four dominant isoforms. Individuals with certain exonic and intronic splice mutations produce additional mutant mRNAs that could be translated into two or more proteins with different amino acid substitutions in a single individual. A fraction of the minor splice variants is predicted to be non-productive. PMID:24342716

  1. Modulation of Splicing by Single-Stranded Silencing RNAs.

    Science.gov (United States)

    Liu, Jing; Hu, Jiaxin; Hicks, Jessica A; Prakash, Thazha P; Corey, David R

    2015-06-01

    Single-stranded silencing RNAs (ss-siRNAs) are chemically modified single-stranded oligonucleotides that can function through the cellular RNA interference (RNAi) machinery to modulate gene expression. Because their invention is recent, few studies have appeared describing their use and the potential of ss-siRNAs as a platform for controlling gene expression remains largely unknown. Using oligonucleotides to modulate splicing is an important area for therapeutic development and we tested the hypothesis that ss-siRNAs targeting splice sites might also be capable of directing increased production of therapeutically promising protein isoforms. Here we observe that ss-siRNAs alter splicing of dystrophin. Altered splicing requires a seed sequence complementarity to the target and expression of the RNAi factor argonaute 2. These results demonstrate that ss-siRNAs can be used to modulate splicing, providing another option for therapeutic development programs that aim to increase production of key protein isoforms. Splicing is a classical nuclear process and our data showing that it can be modulated through the action of RNA and RNAi factors offers further evidence that RNAi can take place in mammalian cell nuclei. PMID:25757055

  2. Width of gene expression profile drives alternative splicing.

    Directory of Open Access Journals (Sweden)

    Daniel Wegmann

    Full Text Available Alternative splicing generates an enormous amount of functional and proteomic diversity in metazoan organisms. This process is probably central to the macromolecular and cellular complexity of higher eukaryotes. While most studies have focused on the molecular mechanism triggering and controlling alternative splicing, as well as on its incidence in different species, its maintenance and evolution within populations has been little investigated. Here, we propose to address these questions by comparing the structural characteristics as well as the functional and transcriptional profiles of genes with monomorphic or polymorphic splicing, referred to as MS and PS genes, respectively. We find that MS and PS genes differ particularly in the number of tissues and cell types where they are expressed.We find a striking deficit of PS genes on the sex chromosomes, particularly on the Y chromosome where it is shown not to be due to the observed lower breadth of expression of genes on that chromosome. The development of a simple model of evolution of cis-regulated alternative splicing leads to predictions in agreement with these observations. It further predicts the conditions for the emergence and the maintenance of cis-regulated alternative splicing, which are both favored by the tissue specific expression of splicing variants. We finally propose that the width of the gene expression profile is an essential factor for the acquisition of new transcript isoforms that could later be maintained by a new form of balancing selection.

  3. “HEALTH & PSYCHOLOGICAL PROBLEMS AMONG S.T. BUS DRIVERS IN MAHARASHTRA"

    OpenAIRE

    Subhash Sadashiv Rane

    2016-01-01

    In State transport bus is one of the main mode of public transport in Maharashtra State. S.T. Bus Drivers have common complaints like gastro-intestinal problems, acute headaches, back pain, neck related problem, knee pain, burning sensation and watering in their eyes. Occupational stress is one of the hazard for many bus drivers. For the present study 100 S. T. drivers were selected through purposive and random sample selection technique. Information pertaining to the present research work ha...

  4. Modeling and simulation of thirty bus system with D-STATCOM for power quality improvement

    Directory of Open Access Journals (Sweden)

    P. Venkata Kishore

    2010-09-01

    Full Text Available This work deals with power quality improvement of thirty bus system using D-STATCOM. The thirty bus system is modeled using the elements of simulink. This model is used for conducting the simulation studies. The simulation results of the thirty bus system with and without D-STATCOM are presented. Power Quality improvement is observed by adding D-STATCOM in the existing network.

  5. The impact of bus drivers ’ lifestyle on the occurrence of health problems and absenteeism

    OpenAIRE

    Andrej Jerman; Maja Meško

    2016-01-01

    Research Question: Do bus drivers’ lifestyles affect the occurrence of health problems and absenteeism? Purpose: The purpose of the study was to determine whether the lifestyle of professional bus drivers affects the occurrence of health problems and the phenomenon of absenteeism. Method: A quantitative research method was used in the research. Data were collected using a structured questionnaire, on a sample of 230 professional bus drivers from differ...

  6. The Bus Extension Module Design and Verification of POSAFE-Q Using LVDS

    International Nuclear Information System (INIS)

    This paper described the Bus Extension Module using Low Voltage Differential Signal (LVDS). A Bus Extension Module use Safety-Related PLC (Programmable Logic Controller), called POSAFE-Q (made by POSCO ICT), for handling many data (I/O) extension rack. A processor module of POSAFE-Q can control I/O Module installed another rack. This paper explains Bus Extension Module and Data transfer technology using LVDS. (author)

  7. Best Operational and Maintenance Practices for City Bus Fleets to Maximize Fuel Economy

    OpenAIRE

    World Bank

    2011-01-01

    In most large cities in developing countries, buses continue to be the public transport option of choice, carrying a large share of urban travelers. However, transit bus companies in these countries are often cash-strapped. In many cases, the operating cost per bus kilometer exceeds revenues and bus fares are often kept low irrespective of the cost of providing service. Many cities are dom...

  8. Field-bus based control-management integrated system for nuclear power plant

    International Nuclear Information System (INIS)

    The characteristics and types of the field-bus as well as its application in Nuclear Power Plant are discussed in this paper, and a new control-management integrated system for nuclear power plant that is based on field-bus is put forward. Analysis and study is carried out in terms of the hierarchy and architecture, network protocol and field-bus type and data information flow. (authors)

  9. Introduction to corridor selection & assessment for Bus Rapid Transit System (BRTS) in Hyderabad

    OpenAIRE

    Bhanu Kireeti Chanda; Maddali Sai Satya Goutham

    2014-01-01

    Bus rapid transit system is an innovation and it is up gradation to the present transport system. BRT is affordable, adaptable and cost effective. The term of rapid transit system means a form of high speed urban passenger transportation. Here, the mode of transportation is bus hence it is called as bus rapid transit system (BRTS). Transportation facilities and the system will be different form different countries. Especially for the developing countries, transportation is a problem and BR...

  10. Reducing carbon emissions by introducing electric vehicle enhanced dedicated bus lanes

    OpenAIRE

    Doolan, Ronan; Muntean, Gabriel-Miro

    2014-01-01

    Most cities have special lanes dedicated to buses, however these lanes are rarely used at full capacity. At the same time governments around the world are encouraging people to buy electric vehicles. This paper proposes the creation of electric vehicle enhanced dedicated bus lanes (E-DBL), by allowing electric vehicles access to bus lanes, in order to improve the use of road capacity. By opening bus lanes to electric vehicles, traffic congestion could be eased, the range ...

  11. BaBar Data Aquisition

    CERN Document Server

    Scott, I; Grosso, P; Hamilton, R T; Huffer, M E; O'Grady, C; Russell, J J

    1998-01-01

    The BaBar experiment at the Stanford Linear Accelerator Center is designed to perform a search for CP violation by analysing the decays of a very large sample of B and Bbar mesons produced at the high luminosity PEP-11 accelerator. The data acquisition system must cope with a sustained high event rate, while supporting real time feature extraction and data compression with minimal dead time. The BaBar data acquisition system is based around a common VME interface to the electronics read-out of the separate detector subsystems. Data from the front end electronics is read into commercial VME processors via a custom "personality card" and PCI interface. The commercial CPUs run the Tornado operating system to provide a platform for detector subsystem code to perform the necessary data processing. The data are read out via a non-blocking network switch to a farm of commercial UNIX processors. Careful design of the core data acquisition code has enabled us to sustain events rates in excess of 20 kHz while maintaini...

  12. Results from bar pp colliders

    International Nuclear Information System (INIS)

    Recent results bar pp colliders are presented. From elastic scattering experiments at the Tevatron, an average value of σtot = 72.1±2 mb is reported, along with a new measurement of ρ = 0.13 ± 0.7. New measurements of jet direct photon and high pt W and Z production are compared to more precise, higher order predictions from perturbative QCD. Recently available data on the W mass and width give combined values for MW = 80.14±0.27 GeV/c2, and Γ(W) =2. 14 ± 0.08 GeV. From electroweak radiative corrections and MW, one finds Mtop = 130±40 GeV/c2, with a 95% C.L. upper limit at 210 GeV/c2. Current limits on Mtop are presented, along with a review of the prospects for top discovery. From jet data there is no evidence of quark substructure down to the distance scale of 1.4 x 10-17 cm, nor is there evidence for supersymmetry or heavy gauge bosons at bar pp colliders, allowing lower limits on MW, > 520 GeV/c2 and MZ 412 GeV/c2. 66 refs., 26 figs

  13. Bitmap-Wise Wireless M-Bus Coordination for Sustainable Real Time Energy Management

    OpenAIRE

    Kwang-il Hwang; Sung-wook Nam

    2014-01-01

    Even though WM-Bus is being considered to be the most promising network protocol for smart metering, it is not suitable for a sustainable real-time home energy management system (HEMS), which requires higher reliability and longer lifetime despite real time bi-directional communications. Therefore, in this paper we propose a Bitmap-wise WM-Bus (BWM-Bus), coping well with sustainable real-time HEMS. In particular, the proposed scheme addresses the several problems in WM-Bus for HEMS by introdu...

  14. A Prediction System Using a P2P Overlay Network for a Bus Arrival System

    Directory of Open Access Journals (Sweden)

    Ssu-Hsuan Lu

    2014-01-01

    Full Text Available Along with the evolution of times and the surge of metropolitan populations, government agencies often promote the construction of public transport. Unlike rail transportation or rapid transit systems, it is often difficult to estimate the vehicle arrival times at each station in a bus transportation system due to metropolitan transportation congestion. Traffic status is often monitored using wireless sensor networks (WSNs. However, WSNs are always separated from one another spatially. Recent studies have considered the connection of multiple sensor networks. This study considers a combination view of peer-to-peer (P2P overlay networks and WSN architecture to predict bus arrival times. Each bus station, which is also a P2P overlay peer, is connected in a P2P overlay network. A sensor installed in each bus can receive data via peers to obtain the moving speed of a bus. Then, each peer can exchange its data to predict bus arrival times at bus stations. This method can considerably increase the accuracy with which bus arrival times can be predicted and can provide traffic status with high precision. Furthermore, these data can also be used to plan new bus routes according to the information gathered.

  15. P{bar P} collider physics

    Energy Technology Data Exchange (ETDEWEB)

    Demarteau, M. [State Univ. of New York, Stony Brook, NY (United States)

    1992-04-01

    A brief introduction to {bar p}p collider physics is given. Selected results from the collider experiments at the CERN S{bar p}pS and the Tevatron collider are described. The emphasis is on experimental aspects of {bar p}p collisions. Minimum bias physics and the production of jets, Intermediate Vector Bosons and heavy flavors is reviewed. The outlook for physics at hadron colliders for the near future is briefly discussed.

  16. Identification of cis-Acting Elements and Splicing Factors Involved in the Regulation of BIM Pre-mRNA Splicing

    OpenAIRE

    Juan, Wen Chun; Roca, Xavier; Ong, S. Tiong

    2014-01-01

    Aberrant changes in the expression of the pro-apoptotic protein, BCL-2-like 11 (BIM), can result in either impaired or excessive apoptosis, which can contribute to tumorigenesis and degenerative disorders, respectively. Altering BIM pre-mRNA splicing is an attractive approach to modulate apoptosis because BIM activity is partly determined by the alternative splicing of exons 3 or 4, whereby exon 3-containing transcripts are not apoptotic. Here we identified several cis-acting elements and spl...

  17. Bus Routes, Prince George's County "The Bus" Bus Routes, Published in 2007, 1:2400 (1in=200ft) scale, Prince George's County Office of Information Technology and Communications.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as...

  18. Bus Stops, Prince George's County "The Bus" Bus Stop locations., Published in 2007, 1:2400 (1in=200ft) scale, Prince George's County Office of Information Technology and Communications.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Stops dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as...

  19. A 5' splice site enhances the recruitment of basal transcription initiation factors in vivo

    DEFF Research Database (Denmark)

    Damgaard, Christian Kroun; Kahns, Søren; Lykke-Andersen, Søren;

    2008-01-01

    promoter docking of transcription initiation factors TFIID, TFIIB, and TFIIH on a gene containing a functional 5′ splice site. In addition to their promoter association, the TFIID and TFIIH components, TBP and p89, are specifically recruited to the 5′ splice site region. Our data suggest a model in which a......Transcription and pre-mRNA splicing are interdependent events. Although mechanisms governing the effects of transcription on splicing are becoming increasingly clear, the means by which splicing affects transcription remain elusive. Using cell lines stably expressing HIV-1 or β-globin m......RNAs, harboring wild-type or various 5′ splice site mutations, we demonstrate a strong positive correlation between splicing efficiency and transcription activity. Interestingly, a 5′ splice site can stimulate transcription even in the absence of splicing. Chromatin immunoprecipitation experiments show enhanced...

  20. The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration

    Science.gov (United States)

    Choudhury, Rajarshi; Roy, Sreerupa Ghose; Tsai, Yihsuan S.; Tripathy, Ashutosh; Graves, Lee M.; Wang, Zefeng

    2014-01-01

    Alternative splicing of pre-messenger RNA (mRNA) is a critical stage of gene regulation in response to environmental stimuli. Here we show that DAZAP1, an RNA-binding protein involved in mammalian development and spermatogenesis, promotes inclusion of weak exons through specific recognition of diverse cis-elements. The carboxy-terminal proline-rich domain of DAZAP1 interacts with and neutralizes general splicing inhibitors, and is sufficient to activate splicing when recruited to pre-mRNA. This domain is phosphorylated by the MEK/Erk (extracellular signal-regulated protein kinase) pathway and this modification is essential for the splicing regulatory activity and the nuclear/cytoplasmic translocation of DAZAP1. Using mRNA-seq, we identify endogenous splicing events regulated by DAZAP1, many of which are involved in maintaining cell growth. Knockdown or over-expression of DAZAP1 causes a cell proliferation defect. Taken together, these studies reveal a molecular mechanism that integrates splicing control into MEK/Erk-regulated cell proliferation.