WorldWideScience

Sample records for bus bar splices

  1. Resistance of LHC main bus bar splices at room temperature and at 77.4 K

    CERN Document Server

    Heck, S; Scheuerlein, Chr; CERN. Geneva. TE Department

    2009-01-01

    As part of the quality control the resistance of newly produced LHC main bus bar splices is now routinely measured at room temperature (RT) in order to conclude on the electrical continuity of the bus bar stabiliser across the splice under operating conditions. In this note we present splice resistance measurements that have been performed at RT and in liquid nitrogen (LN) in the CERN Cryolab with “ideal” splices (represented by continuous dipole and quadrupole bus bars), and with dipole and quadrupole splices with different defects, which cause an additional RT splice resistance of up to 60 µΩ. The RT resistance (RRT) results obtained with the Cryolab set-up are compared to the calculated resistance values and with the so-called R-8 and R-16 resistance results, as they are measured in the LHC tunnel with a Digital Low Resistance Ohmmeter with a voltage tap distance of 8 cm or 16 cm. The RT to LN resistance ratio has been determined for all splices in order to study the influence of the resistance of th...

  2. A train for the bus(bars)

    CERN Multimedia

    CERN Bulletin

    2013-01-01

    On 8 April, the first SMACC (Superconducting Magnets and Circuits Consolidation) teams began work in the LHC tunnel. They are responsible for opening the interconnects between the magnets, laying the groundwork for the series of operations that will be performed by the team riding the ‘consolidation train’.   A technician installs the machine tool that allows them to prepare the surface of the section of the bar where the shunt will be fixed. The LHC’s 1,670 dipoles and quadrupoles are powered by power converters and connected by copper 'busbars’. The superconducting cables run through these bars, carrying a current of up to 11,850 amps. Six superconducting cables meet at each interconnect, where they are held together by a soldered (see box) electrical connection sandwiched between two pieces of copper, forming the splice between the busbars of the neighbouring magnets. The integrity of the electrical circuit is depen...

  3. Bus bar electrical feedthrough for electrorefiner system

    Science.gov (United States)

    Williamson, Mark; Wiedmeyer, Stanley G; Willit, James L; Barnes, Laurel A; Blaskovitz, Robert J

    2013-12-03

    A bus bar electrical feedthrough for an electrorefiner system may include a retaining plate, electrical isolator, and/or contact block. The retaining plate may include a central opening. The electrical isolator may include a top portion, a base portion, and a slot extending through the top and base portions. The top portion of the electrical isolator may be configured to extend through the central opening of the retaining plate. The contact block may include an upper section, a lower section, and a ridge separating the upper and lower sections. The upper section of the contact block may be configured to extend through the slot of the electrical isolator and the central opening of the retaining plate. Accordingly, relatively high electrical currents may be transferred into a glovebox or hot-cell facility at a relatively low cost and higher amperage capacity without sacrificing atmosphere integrity.

  4. Black silicon solar cells with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by maskless reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Four different methods to obtain blackened bus-bar strings were compared with respect to reflectance, and two of these methods (i.e., oxidized copper and etched solder) were used to fabricate functional allblack solar 9-cell panels. The black bus-bars (e.......g., by oxidized copper) have a reflectance below 3% in the entire visible wavelength range. The combination of black silicon cells and blackened bus-bars results in aesthetic, all-black panels based on conventional, front-contacted solar cells without compromising efficiency....

  5. Black silicon with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by mask-less reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Black bus-bars were realized by oxidized copper resulting in reflectance below 3% in the entire visible wavelength range. The combination of these two technologies may result in aesthetic, all-black panels based on conventional, front-contacted solar cells...

  6. Mechanical analysis of the main bus bars in the DFBA shuffling modules

    CERN Document Server

    Ramos, D

    2012-01-01

    The main bus bars (13 kA) inside the shuffling modules of the distribution feed boxes powering the LHC arcs (DFBA) are subjected to high Lorentz forces. The structural behaviour of the bus bars under such forces is here analysed. The results are discussed with respect to a risk of structural failure due to excessive deformation or degradation of the electrical insulation by repeated contact with other surfaces.

  7. An analytical algorithm to determine allowable ampacities of horizontally installed rectangular bus bars

    Directory of Open Access Journals (Sweden)

    Klimenta Dardan O.

    2016-01-01

    Full Text Available The main objective of this paper is to propose an algorithm for the determination of the allowable ampacities of single rectangular-section bus bars without the occurrence of correction factors. Without correction factors, the ampacity computation of the copper and aluminium bus bars is fully automatized. The analytical algorithm has been implemented in a computer program code that along with the allowable ampacity can compute the bus bar temperature and the individual heat transfer coefficient for each side of the bus bar, as well as their corresponding power losses. Natural and forced convection correlations for rectangular bus bars are applied. Effects of the solar radiation and radiation heat losses from the bus bar surface are taken into consideration as well. The finite element method (FEM has been used for the linear/non-linear steady-state thermal analysis, i.e. for validation of the analytical algorithm. All FEM-based numerical computations were carried out using the COMSOL Heat Transfer Module. [Projekat Ministarstva nauke Republike Srbije, br. TR33046

  8. The high current bus-bars of the LHC from conception to manufacture

    CERN Document Server

    Belova, L; Perinet-Marquet, J-L; Urpin, C

    2006-01-01

    The main magnets of the LHC are series-connected electrically in different excitation circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper section all along length. The function of the copper section is essentially to provide an alternative path for the magnet current in case the superconducting cable loses its superconductivity and returns to normal state because of a transient system disturbance or normal zone propagation coming from the neighboring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100 ° C while the copper is conducting. With regard to that, a quench signal is initiated, which in turn triggers the ramping down of the current from 13000 A to 0....

  9. Impedance of bus bars: influence of the design characteristics and results of measurements; Impedancia de barramentos blindados: influencia das caracteristicas construtivas e resultados praticos de medicoes

    Energy Technology Data Exchange (ETDEWEB)

    Bomeisel, Eduardo [Institute of Electrical and Electronics Engineers (IEEE), Orlando, FL (United States); D' Avila, Ricardo Santos [Universidade de Sao Paulo (LAC/IEE/USP), SP (Brazil). Inst. de Eletrotecnica e Energia. Lab. de Altas Correntes

    2008-12-15

    This article focuses on bus bars, used in industrial and building installations, and is based on bus bars for the following application: power distribution, sheltered environments, low voltage (<1000 V) phase 60Hz.

  10. Design and Manufacture of the Superconducting Bus-bars for the LHC Main Magnets

    CERN Document Server

    Belova, L M; Perinet-Marquet, J L; Ivanov, P; Urpin, C

    2002-01-01

    The main magnets of the LHC are series-connected electrically in different powering circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper profile all along the length. The function of the copper profile is essentially to provide an alternative path for the current in case the superconducting cable loses its superconducting state and returns to normal state because of a transient disturbance or of a normal zone propagation coming from the neighbouring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100°C while the copper is conducting. When a resistive transition is detected, the protection systems triggers the ramping down of the current from 13000 A to 0. The ramp rate must not exceed a maximum value to avoid the transition of magnets series-connected in the circuit. This paper concerns th...

  11. Partial Stray Inductance Modeling and Measuring of Asymmetrical Parallel Branches on the Bus-Bar of Electric Vehicles

    Directory of Open Access Journals (Sweden)

    Chengfei Geng

    2017-10-01

    Full Text Available In order to increase the power rating of electric vehicles, insulated gate bipolar translator (IGBT modules with multiple power terminals are usually adopted. The transient current sharing of the same polarity power terminals is related to the stray inductance in the branches of the bus-bar. Based on the laminated bus-bar of a three-phase inverter in the electric vehicles that consists of asymmetrical parallel branches, this paper investigates the transient current imbalance sharing caused by the asymmetrical stray inductance in the parallel branches of the bus-bar from the view of energy storing and releasing of stray inductance for the first time. Besides, the partial self-inductance and mutual-inductance model of the parallel branches is set up. Finally, a high-precision partial stray inductance measurement method is proposed, and the accuracy of the partial stray inductance model for asymmetrical parallel branches is verified by experimental tests.

  12. Thermal Performance of the LHC External Auxiliary Bus-Bar Tube Mathematical Modelling

    CERN Document Server

    Kowalczyk, P; Sacré, P; Skoczen, Blazej

    1998-01-01

    The Large Hadron Collider (LHC) externally routed auxiliary bus-bar tube (EAB) will house the electrical feeders of the LHC short straight section (SSS) correcting magnets. The superconducting wires w ill be contained in a stainless steel tube and immersed in a quasi-static helium bath. The EAB thermal performance during the cooling of the magnets down to the operating temperature of 1.9 K is studi ed. A 3-d finite element thermal model of the EAB during a cooling process from 293 K to 4.5 K is described. The semi-analytical model of the EAB cool-down from 4.5 K to 1.9 K is also presented.

  13. High-Current Bus Splice Resistances and Implications for the Operating Energy of the LHC

    CERN Document Server

    Koratzinos, M; Charifoulline, Z; Dahlerup-Petersen, K; Denz, R; Flora, R H; Pfeffer, H; Scheuerlein, C; Schmidt, R; Siemko, A; Strait, J; Verweij, A

    2010-01-01

    At each interconnection between LHC main magnets a low-resistance solder joint must be made between superconducting cables in order to provide a continuous current path through the superconductor and also to the surrounding copper stabilizer in case the cable quenches [1]. About 10,000 such joints exist in the LHC. An extensive campaign has been undertaken to characterize and map the resistances of these joints. All of the superconducting cable splices were measured at 1.9 K and no splices were found with a resistance larger than 3 nW. Non-invasive measurements of the stabilizer joints were made at 300 K in 5 of the 8 sectors, and at 80 K in 3 sectors. More precise local measurements were made on suspect interconnects that were opened up, and poor joints were repaired. However, it is likely that additional imperfect stabilizer joints still exist in the LHC. A statistical analysis is used to place bounds on the remaining worst-case resistances. This sets limits on the maximum operating energy of the LHC, prior...

  14. Proposal for the award of a contract for the supply of insulating profiles for the LHC bus bars

    CERN Document Server

    2001-01-01

    This document concerns the award of a contract for the supply of insulating profiles, of three different types and two different lengths, for the LHC bus bars. Following a market survey carried out among 120 firms in sixteen Member States, a call for tenders (IT-2656/LHC/LHC) was sent on 30 May 2001 to 17 firms in ten Member States. By the closing date, CERN had received nine tenders from nine firms in six Member States. The Finance Committee is invited to agree to the negotiation of a contract with EXEL (FI), the lowest bidder, for the supply of 16 260 insulating profiles, of three different types and two different lengths, for the LHC bus bars for a total amount of 789 764 Swiss francs, not subject to revision until 1 January 2003, with an option for up to 10% additional insulating profiles, for an additional amount of 78 976 Swiss francs, not subject to revision until 1 January 2003, bringing the total amount to 868 740 Swiss francs, not subject to revision until 1 January 2003. The firm has indicated the ...

  15. Modelling of thermal power plant for the needs of Q-V characteristic control on HV bus bars

    Directory of Open Access Journals (Sweden)

    Pavlović Jelena

    2015-01-01

    Full Text Available In this paper, a mathematical model of the thermal power plants TENT A and TENT B, developed for voltage and reactive power control simulation purposes, is presented. The model is developed using the Simulink/Matlab program platform. All important elements, such as synchronous generator, step-up transformer, turbine and governor, excitation system, automatic voltage regulator (ARN and the network, connected to the power plant's bus bars, are modelled with the complexity level required for studying the problems of interest. In the first part, a review of the hierarchical voltage control in the system is given, because the coordinated reactive power regulator (GRRS has to be embedded in future processes of voltage control automatisation on higher levels and 'smart' control in the power system. For the needs of studying the HV bus bars voltage control, as well as reactive power allocation within the power plant in manual and automatic control mode, a series of simulations was performed. For verification purposes, the simulation results are compared to the on-site measurements. An excellent match of simulated and recorded results is achieved.

  16. Heat generation by eddy currents in a shell of superconducting bus-bars for SIS100 particle accelerator at FAIR

    Directory of Open Access Journals (Sweden)

    Tomków Łukasz

    2017-12-01

    Full Text Available Superconducting magnets in the SIS100 particle accelerator require the supply of liquid helium and electric current. Both are transported with by-pass lines designed at Wrocław University of Technology. Bus-bars used to transfer an electric current between the sections of the accelerator will be encased in a steel shell. Eddy currents are expected to appear in the shell during fast-ramp operation of magnets. Heat generation, which should be limited in any cryogenic system, will appear in the shell. In this work the amount of heat generated is assessed depending on the geometry of an assembly of the bus-bars and the shell. Numerical and analytical calculations are described. It was found that heat generation in the shell is relatively small when compared to other sources present in the accelerator and its value strongly depends on the geometry of the shell. The distribution of eddy currents and generated heat for different geometrical options are presented. Based on the results of the calculations the optimal design is proposed.

  17. Proposal for the award of a blanket contract for the supply and installation of water-cooled bus bars and cables for the LHC

    CERN Document Server

    2003-01-01

    This document concerns the award of a blanket contract for the supply and installation of water-cooled bus bars and cables for the LHC project. Following a market survey carried out among 22 firms in six Member States, a call for tenders (IT-2941/ST/LHC) was sent on 30 June 2003 to three firms in two Member States. By the closing date, CERN had received tenders from the three firms. The Finance Committee is invited to agree to the negotiation of a blanket contract with FLOHE (DE), the lowest bidder, for the supply and installation of water-cooled bus bars and cables, for a total amount not exceeding 2 900 000 Swiss francs, subject to revision after 1 January 2005 according to the LME copper prices. The firm has indicated the following distribution by country of the contract value covered by this adjudication proposal: DE - 100%.

  18. Measurement of the Residual Resistivity Ratio of the Bus Bars Copper Stabilizer of the 13 kA Circuits of the LHC

    CERN Document Server

    Apollonio, A; Solfaroli Camillocci, M; Schmidt, R; Verweij, A; Siemko, A; Claudet, S; Steckert, J; Thiesen, H

    2012-01-01

    After the incident of September 2008, the operational beam energy of the LHC has been set to 3.5 TeV, since not all joints of the superconducting (SC) bus bars between magnets have the required quality for 7 TeV operation. This decision is based on simulations to determine the safe current in the main dipole and quadrupole circuits, reproducing the thermal behaviour of a quenched superconducting joint by taking into account all relevant factors that affect a possible thermal runaway. One important parameter is the Residual Resistivity Ratio (RRR) of the copper stabilizer of the bus bar connecting the superconducting magnets. A dedicated campaign to measure the RRR for the main 13 kA circuits of the LHC in all sectors was performed during the Christmas stop in December 2010 and January 2011. The measurement method as well as the data analysis and results are presented in this paper.

  19. Copper Heat Exchanger for the External Auxiliary Bus-Bars Routing Line in the LHC Insertion Regions

    CERN Document Server

    Garion, C; Seyvet, F; Sitko, M; Skoczen, B; Tock, J P

    2006-01-01

    The corrector magnets and the main quadrupoles of the LHC dispersion suppressors are powered by a special superconducting line (called auxiliary bus-bars line N), external to the cold mass and housed in a 50 mm diameter stainless steel tube fixed to the cold mass. As the line is periodically connected to the cold mass, the same gaseous and liquid helium cools both the magnets and the line. The final sub-cooling process (from around 4.5 K down to 1.9 K) consists in the phase transformation from liquid to superfluid helium. Heat is extracted from the line through the magnets via their point of junction. In dispersion suppressor zones, approximately 40 m long, the sub-cooling of the line is slightly delayed with respect to the magnets. This might have an impact on the readiness of the accelerator for operation. In order to accelerate the process, a special heat exchanger has been designed. It is located in the middle of the dispersion suppressor portion of the line. Its main function consists in providing a loca...

  20. Splice Resistance Measurements in the LHC Main Superconducting Magnet Circuits by the New Quench Protection System

    CERN Document Server

    Charifoulline, Z; Denz, R; Siemko, A; Steckert, J

    2012-01-01

    The interconnections between the LHC main magnets are made of soldered joints (splices) of two superconducting cables stabilized by a copper bus-bar. After the 2008 LHC incident, caused by a defective interconnection, a new layer of high resolution magnet circuit quench protection (nQPS) has been developed and integrated with the existing systems. It allowed mapping of the resistances of all superconducting splices during the 2009 commissioning campaign. Since April 2010, when the LHC was successfully restarted at 3.5 TeV, every bus bar interconnection is constantly monitored by the nQPS electronics. The acquired data are saved to the LHC Logging Database. The paper will briefly describe the data analysis method and will present the results from the two years of resistance measurements. Although no splice was found with resistance higher than 3.3 n and no significant degradation in time was observed so far, the monitoring of splices will stay active till the end of LHC 4 TeV run. The detected outliers wil...

  1. A close-up of the lower part of a 13 kA current lead. The high-temperature superconductor (on the left in the photo) with the low-temperature superconductor (on the right). Resting in liquid helium, the low-temperature superconductor is connected to the bus-bars conveying the current to the LHC magnets.

    CERN Multimedia

    2004-01-01

    A close-up of the lower part of a 13 kA current lead. The high-temperature superconductor (on the left in the photo) with the low-temperature superconductor (on the right). Resting in liquid helium, the low-temperature superconductor is connected to the bus-bars conveying the current to the LHC magnets.

  2. ELECTRO-THERMAL AND MECHANICAL VALIDATION EXPERIMENT ON THE LHC MAIN BUSBAR SPLICE CONSOLIDATION

    CERN Document Server

    Willering, GP; Bourcey, N; Bottura, L; Charrondiere, M; Cerqueira Bastos, M; Deferne, G; Dib, G; Giloux, Chr; Grand-Clement, L; Heck, S; Hudson, G; Kudryavtsev, D; Perret, P; Pozzobon, M; Prin, H; Scheuerlein, Chr; Rijllart, A; Triquet, S; Verweij, AP

    2012-01-01

    To eliminate the risk of thermal runaways in LHC interconnections a consolidation by placing shunts on the main bus bar interconnections is proposed by the Task Force Splices Consolidation. To validate the design two special SSS magnet spares are placed on a test bench in SM-18 to measure the interconnection in between with conditions as close as possible to the LHC conditions. Two dipole interconnections are instrumented and prepared with worst-case-conditions to study the thermo-electric stability limits. Two quadrupole interconnections are instrumented and prepared for studying the effect of current cycling on the mechanical stability of the consolidation design. All 4 shunted interconnections showed very stable behaviour, well beyond the LHC design current cycle.

  3. GO Bus impact analysis.

    Science.gov (United States)

    2013-08-01

    NJ TRANSIT launched two relatively new enhanced bus services: GO 25 serving the : Springfield Avenue corridor in 2008 and GO 28 serving the Bloomfield Avenue corridor in 2009. : As an enhanced but not full Bus Rapid Transit (BRT) service, GO Bus feat...

  4. Effect of tension lap splice on the behavior of high strength concrete (HSC beams

    Directory of Open Access Journals (Sweden)

    Ahmed El-Azab

    2014-12-01

    Full Text Available In the recent years, many research efforts have been carried out on the bond strength between normal strength concrete (NSC and reinforcing bars spliced in tension zones in beams. Many codes gave a minimum splice length for tension and compression reinforcement as a factor of the bar diameter depending on many parameters such as concrete strength, steel yield stress, shape of bar end, shape of bar surface and also bar location. Also, codes gave another restriction about the percentage of total reinforcement to be spliced at the same time. Comparatively limited attention has been directed toward the bond between high strength concrete (HSC and reinforcing bars spliced in tension zones in beams. HSC has high modulus of elasticity, high density and long-term durability. This research presents an experimental study on the bond between high strength concrete (HSC and reinforcing bars spliced in tension zones in beams. It reports the influence of several parameters on bond in splices. The parameters covered are casting position, splice length as a factor of bar diameter, bar diameter and reinforcement ratio. The research involved tests on sixteen simply-supported beams of 1800 mm span, 200 mm width and 400 mm thickness made of HSC. In each beam, the total tensile steel bars were spliced in the constant moment zone. Crack pattern, crack propagation, cracking load, failure load and mi span deflection were recorded and analyzed to study the mentioned parameters effect.

  5. PCI bus demystified

    CERN Document Server

    Abbott, Doug

    2004-01-01

    The peripheral component interconnect (PCI) bus is the dominant bus system used to connect the different elements making up today''s high-performance computer systems. Different PCI implementations have also been developed for such applications as telecommunications and embedded computing. If an application calls for high speed, high reliability, flexible configuration, and bus mastering, then PCI is the only logical bus choice. This book is an applications-oriented introduction to the PCI bus, with an emphasis on implementing PCI in a variety of computer architectures. Special attention is given to industrial and mission-critical applications of PCI bus.·Fully describes PCI electrical specifications, mechanical requirements, and signal types·Covers advanced topics through numerous design examples to increase the readers understanding of the subject ·Includes updated coverage of PCI-X 2.0

  6. Intelligent Bus Stops in the Flexible Bus Systems

    Directory of Open Access Journals (Sweden)

    Razi Iqbal

    2014-09-01

    Full Text Available The purpose of this paper is to discuss Intelligent Bus Stops in a special Demand Responsive Transit (DRT, the Flexible Bus System. These Intelligent Bus Stops are more efficient and information rich than Traditional Bus Stops. The real time synchronization of the Flexible Bus System makes it unique as compared to Traditional Bus Systems. The Main concern is to make Bus Stops intelligent and information rich. Buses are informed about the no. of passengers waiting at the upcoming Bus Stops. If there are no passengers to ride or get off on upcoming Bus Stop, the Bus can skip that Bus Stop and head towards the next Bus Stop where passenger is waiting, which will decrease the ride time of the passengers on the Bus and also the wait time of the passengers waiting on the upcoming Bus Stops. Providing more information at Bus Stops about the Destination (Time to Destination, Distance to Destination etc. and Buses (Bus Location, Arrival Time of Bus etc. makes it easier for the passengers to decide whether to ride a particular Bus or not.

  7. Effect of fiber blending ratios of cotton/polyester yarns on retained splice diameter

    Science.gov (United States)

    Çelik, H. İ.; Kaynak, H. K.

    2017-10-01

    The most important performance parameters of splicing are obtaining adequate strength and appearance at the splice point for all processing requirements. The diameter of spliced portion effects not only appearance of the splice joints but also physical characteristics such as packing density, strength, specific volume of the yarn. In this study, the effect of cotton/polyester fiber blend ratios on spliced portion diameter at different slicing air pressures was investigated. For this aim, three yarn samples 100% cotton, 80-20% CO-PES and 50- 50% CO-PES were produced with 40/1 Ne. Each yarn samples was spliced at three different pressures; 4 bar, 5 bar and 6 bar. The diameters of spliced portion and retained yarns were measured by using ImageJ program and the results were analyzed statistically.

  8. TPG bus passes

    CERN Multimedia

    Staff Association

    2013-01-01

    The CERN Staff Association will stop selling TPG bus passes. All active and retired members of the CERN personnel will be able to purchase Unireso bus passes from the CERN Hostel - Building 39 (Meyrin site) from 1st February 2013. For more information: https://cds.cern.ch/journal/CERNBulletin/2013/04/Announcements/1505279?ln=en

  9. Electric bus systems.

    Science.gov (United States)

    2017-04-01

    Pure electric buses (EBs) offer an alternative fuel for the nations transit bus systems. To : evaluate EBs in a transit setting, this project investigated the five electric bus fleet of the : StarMetro transit system of the city of Tallahassee, FL...

  10. Savannah River bus project

    Energy Technology Data Exchange (ETDEWEB)

    Summers, W.A. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1998-08-01

    The H2Fuel Bus is the world`s first hybrid hydrogen electric transit bus. It was developed through a public/private partnership involving several leading technology and industrial organizations in the Southeast, with primary funding and program management provided by the Department of Energy. The primary goals of the project are to gain valuable information on the technical readiness and economic viability of hydrogen buses and to enhance the public awareness and acceptance of emerging hydrogen technologies. The bus has been operated by the transit agency in Augusta, Georgia since April, 1997. It employs a hybrid IC engine/battery/electric drive system, with onboard hydrogen fuel storage based on the use of metal hydrides. Initial operating results have demonstrated an overall energy efficiency (miles per Btu) of twice that of a similar diesel-fueled bus and an operating range twice that of an all-battery powered electric bus. Tailpipe emissions are negligible, with NOx less than 0.2 ppm. Permitting, liability and insurance issues were addressed on the basis of extensive risk assessment and safety analyses, with the inherent safety characteristic of metal hydride storage playing a major role in minimizing these concerns. Future plans for the bus include continued transit operation and use as a national testbed, with potential modifications to demonstrate other hydrogen technologies, including fuel cells.

  11. LHC on the bus

    CERN Multimedia

    Laëtitia Pedroso

    2010-01-01

    On 15 December, an airport bus will be transformed in the image of CERN. The bus will be seen by the thousands of travellers arriving in Geneva, informing them of the possibility to visit CERN.   Sketch of the bus. The good relationship between Geneva International Airport and CERN started several years ago. In 2004 the airport put advertising space in the arrivals area at CERN's disposal free of charge. Now, starting on 15 December, a 40-foot long bus will display a giant sticker advertisement depicting CERN as it takes passengers over the airport tarmac to their planes. This is no ordinary sticker, and it was no mean task to attach it to the bus. The task of producing and attaching it was entrusted to Geneva-based specialists Mathys SA. With the ski season opening on 15 December, there will be many travellers arriving at the airport, and the bus will be ready to receive them. When one thinks of CERN, the subjects that naturally come to mind are the LHC, the mysteries of the Universe...

  12. Alternative Splicing in CKD

    OpenAIRE

    Stevens, Megan; Oltean, Sebastian

    2016-01-01

    Alternative splicing (AS) has emerged in the postgenomic era as one of the main drivers of proteome diversity, with ���94% of multiexon genes alternatively spliced in humans. AS is therefore one of the main control mechanisms for cell phenotype, and is a process deregulated in disease. Numerous reports describe pathogenic mutations in splice factors, splice sites, or regulatory sequences. Additionally, compared with the physiologic state, disease often associates with an abnormal proportion o...

  13. Learning NServiceBus

    CERN Document Server

    Boike, David

    2013-01-01

    This is a practical tutorial containing hands-on examples for creating a messaging and SOA based service bus.This book is for .NET developers who are looking for ways to overcome problems related to buggy third party web service integrations, codebases that have grown into a big ball of mud, and batch jobs failure.

  14. Nonlinear Analysis of Spliced Continuous RC Girders Strengthened with (CFRP Laminates using ANSYS

    Directory of Open Access Journals (Sweden)

    Ammar Yasir Ali

    2016-03-01

    Full Text Available This paper presents the details of the finite element analysis of spliced continuous reinforced concrete girders. Five spliced continuous girders and one non-spliced continuous girder were analyzed using the ANSYS program. Each spliced girder consisted of three precast segments spliced at two cast-in-place joints at the inflection points, using splices of hooked dowels. Three spliced girders were strengthened using different schemes of the carbon fiber reinforced polymer (CFRP laminates. The concrete was modeled using (SOLID65 eight-node brick element and the steel reinforcement was modeled discretely using (LINK8 spar element. The straight parts of the spliced bars were modeled using discrete representation with interface elements using (COMBIN39 elements to represent the bond-slip behavior while the hooked part of each spliced bar was replaced by a single spring element. The CFRP laminates were modeled using (SHELL41 shell element. The interfaces between the precast concrete segments and the joints were modeled using CONTAC52 interface elements in conjunction with vertical spring elements to represent the dowel action of the steel bars that crossing the interfaces. The ANSYS model succeeded to an acceptable degree in predicting the structural behavior of the analyzed spliced girders with average of differences of about 6% between the predicted and experimental ultimate load.

  15. Neuron-specific splicing.

    Science.gov (United States)

    Hakim, Nor Hakimah Ab; Majlis, Burhanuddin Yeop; Suzuki, Hitoshi; Tsukahara, Toshifumi

    2017-03-22

    During pre-mRNA splicing events, introns are removed from the pre-mRNA, and the remaining exons are connected together to form a single continuous molecule. Alternative splicing is a common mechanism for the regulation of gene expression in eukaryotes. More than 90% of human genes are known to undergo alternative splicing. The most common type of alternative splicing is exon skipping, which is also known as cassette exon. Other known alternative splicing events include alternative 5' splice sites, alternative 3' splice sites, intron retention, and mutually exclusive exons. Alternative splicing events are controlled by regulatory proteins responsible for both positive and negative regulation. In this review, we focus on neuronal splicing regulators and discuss several notable regulators in depth. In addition, we have also included an example of splicing regulation mediated by the RBFox protein family. Lastly, as previous studies have shown that a number of splicing factors are associated with neuronal diseases such as Alzheime's disease (AD) and Autism spectrum disorder (ASD), here we consider their importance in neuronal diseases wherein the underlying mechanisms have yet to be elucidated.

  16. Alternative splicing in ascomycetes.

    Science.gov (United States)

    Kempken, Frank

    2013-05-01

    Alternative splicing is a complex and regulated process, which results in mRNA with different coding capacities from a single gene. Extend and types of alternative splicing vary greatly among eukaryotes. In this review, I focus on alternative splicing in ascomycetes, which in general have significant lower extend of alternative splicing than mammals. Yeast-like species have low numbers of introns and consequently alternative splicing is lower compared to filamentous fungi. Several examples from single studies as well as from genomic scale analysis are presented, including a survey of alternative splicing in Neurospora crassa. Another focus is regulation by riboswitch RNA and alternative splicing in a heterologous system, along with putative protein factors involved in regulation.

  17. Modeling the Coordinated Operation between Bus Rapid Transit and Bus

    Directory of Open Access Journals (Sweden)

    Jiaqing Wu

    2015-01-01

    Full Text Available The coordination between bus rapid transit (BRT and feeder bus service is helpful in improving the operational efficiency and service level of urban public transport system. Therefore, a coordinated operation model of BRT and bus is intended to develop in this paper. The total costs are formulated and optimized by genetic algorithm. Moreover, the skip-stop BRT operation is considered when building the coordinated operation model. A case of the existing bus network in Beijing is studied, the proposed coordinated operation model of BRT and bus is applied, and the optimized headway and costs are obtained. The results show that the coordinated operation model could effectively decrease the total costs of the transit system and the transfer time of passengers. The results also suggest that the coordination between the skip-stop BRT and bus during peak hour is more effective than non-coordination operation.

  18. Alternative Splicing in CKD.

    Science.gov (United States)

    Stevens, Megan; Oltean, Sebastian

    2016-06-01

    Alternative splicing (AS) has emerged in the postgenomic era as one of the main drivers of proteome diversity, with ≥94% of multiexon genes alternatively spliced in humans. AS is therefore one of the main control mechanisms for cell phenotype, and is a process deregulated in disease. Numerous reports describe pathogenic mutations in splice factors, splice sites, or regulatory sequences. Additionally, compared with the physiologic state, disease often associates with an abnormal proportion of splice isoforms (or novel isoforms), without an apparent driver mutation. It is therefore essential to study how AS is regulated in physiology, how it contributes to pathogenesis, and whether we can manipulate faulty splicing for therapeutic advantage. Although the disease most commonly linked to deregulation of AS in several genes is cancer, many reports detail pathogenic splice variants in diseases ranging from neuromuscular disorders to diabetes or cardiomyopathies. A plethora of splice variants have been implicated in CKDs as well. In this review, we describe examples of these CKD-associated splice variants and ideas on how to manipulate them for therapeutic benefit. Copyright © 2016 by the American Society of Nephrology.

  19. BBIS: Beacon Bus Information System

    Science.gov (United States)

    Kasim, Shahreen; Hafit, Hanayanti; Pei Juin, Kong; Afizah Afif, Zehan; Hashim, Rathiah; Ruslai, Husni; Jahidin, Kamaruzzaman; Syafwan Arshad, Mohammad

    2016-11-01

    Lack of bus information for example bus timetable, status of the bus and messy advertisement on bulletin board at the bus stop will give negative impact to tourist. Therefore, a real-time update bus information bulletin board provides all information needed so that passengers can save their bus information searching time. Supported with Android or iOS, Beacon Bus Information System (BBIS) provides bus information between Batu Pahat and Kluang area. BBIS is a system that implements physical web technology and interaction on demand. It built on Backend-as-a-Service, a cloud solution and Firebase non relational database as data persistence backend and syncs between user client in the real-time. People walk through bus stop with smart device and do not require any application. Bluetooth Beacon is used to achieve smart device's best performance of data sharing. Intellij IDEA 15 is one of the tools that that used to develop the BBIS system. Multi-language included front end and backend supported Integration development environment (IDE) helped to speed up integration process.

  20. Bus lane efficiency analysis

    OpenAIRE

    Tovornik, Peter

    2006-01-01

    The upper Celovška road has been dealing with traffic jams for quite some time now. Traffic jams are concentrated in time of morning (in direction towards towns center) and afternoon (in direction towards Gorenjska) peak hour , whereas jams in between times are not excluded. There are many solutions to solve the problem and are variable. In this assigment I am focusing on one of potencial solutions, with special bus lanes. It is a measure that can be, if used in right way, very effective and ...

  1. Splicing Regulation in Neurologic Disease

    National Research Council Canada - National Science Library

    Licatalosi, Donny D; Darnell, Robert B

    2006-01-01

    .... It is becoming evident that alternative splicing plays a particularly important role in neurologic disease, which is perhaps not surprising given the important role splicing plays in generating...

  2. spliceR

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Porse, Bo Torben; Sandelin, Albin

    2014-01-01

    RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternative splicing...

  3. Learning NServiceBus Sagas

    CERN Document Server

    Helton, Rich

    2015-01-01

    If you are an Enterprise C# developer who wishes to extend your knowledge of NServiceBus and Enterprise Service Bus in C#, this is the book for you. This book is designed to enhance the education of ESBs and their messaging, whether you are a beginner or a seasoned expert in Enterprise C#, Apex, and Visualforce pages.

  4. Performance of Grouted Splice Sleeve Connector under Tensile Load

    Directory of Open Access Journals (Sweden)

    A. Alias

    2016-05-01

    Full Text Available The grouted splice sleeve connector system takes advantage of the bond-slip resistance of the grout and the mechanical gripping of reinforcement bars to provide resistance to tensile force. In this system, grout acts as a load-transferring medium and bonding material between the bars and sleeve. This study adopted the end-to-end rebars connection method to investigate the effect of development length and sleeve diameter on the bonding performance of the sleeve connector. The end-to-end method refers to the condition where reinforcement bars are inserted into the sleeve from both ends and meet at the centre before grout is filled. Eight specimens of grouted splice sleeve connector were tested under tensile load to determine their performance. The sleeve connector was designed using 5 mm thick circular hollow section (CHS steel pipe and consisted of one external and two internal sleeves. The tensile test results show that connectors with a smaller external and internal sleeve diameter appear to provide better bonding performance. Three types of failure were observed in this research, which are bar fracture (outside the sleeve, bar pullout, and internal sleeve pullout. With reference to these failure types, the development length of 200 mm is the optimum value due to its bar fracture type, which indicates that the tensile capacity of the connector is higher than the reinforcement bar. It is found that the performance of the grouted splice sleeve connector is influenced by the development length of the reinforcement bar and the diameter of the sleeve.

  5. Optical Fiber Fusion Splicing

    CERN Document Server

    Yablon, Andrew D

    2005-01-01

    This book is an up-to-date treatment of optical fiber fusion splicing incorporating all the recent innovations in the field. It provides a toolbox of general strategies and specific techniques that the reader can apply when optimizing fusion splices between novel fibers. It specifically addresses considerations important for fusion splicing of contemporary specialty fibers including dispersion compensating fiber, erbium-doped gain fiber, polarization maintaining fiber, and microstructured fiber. Finally, it discusses the future of optical fiber fusion splicing including silica and non-silica based optical fibers as well as the trend toward increasing automation. Whilst serving as a self-contained reference work, abundant citations from the technical literature will enable readers to readily locate primary sources.

  6. Chromatin and alternative splicing.

    Science.gov (United States)

    Alló, M; Schor, I E; Muñoz, M J; de la Mata, M; Agirre, E; Valcárcel, J; Eyras, E; Kornblihtt, A R

    2010-01-01

    Alternative splicing affects more than 90% of human genes. Coupling between transcription and splicing has become crucial in the complex network underlying alternative splicing regulation. Because chromatin is the real template for nuclear transcription, changes in its structure, but also in the "reading" and "writing" of the histone code, could modulate splicing choices. Here, we discuss the evidence supporting these ideas, from the first proposal of chromatin affecting alternative splicing, performed 20 years ago, to the latest findings including genome-wide evidence that nucleosomes are preferentially positioned in exons. We focus on two recent reports from our laboratories that add new evidence to this field. The first report shows that a physiological stimulus such as neuron depolarization promotes intragenic histone acetylation (H3K9ac) and chromatin relaxation, causing the skipping of exon 18 of the neural cell adhesion molecule gene. In the second report, we show how specific histone modifications can be created at targeted gene regions as a way to affect alternative splicing: Using small interfering RNAs (siRNAs), we increased the levels of H3K9me2 and H3K27me3 in the proximity of alternative exon 33 of the human fibronectin gene, favoring its inclusion into mature messenger RNA (mRNA) through a mechanism that recalls RNA-mediated transcriptional gene silencing.

  7. 75 FR 52054 - Bus and Bus Facilities Discretionary Program Funds

    Science.gov (United States)

    2010-08-24

    ... 8, 2009. The Livability Bus program makes funds available to public transit providers to finance... 2-New York, Administrator, Region 7-Kansas One Bowling Green, Room 429, New City, MO, 901 Locust... Metropolitan Office, Region 2-New York, One Bowling Green, Room 428, New York, NY 10004-1415, Tel. 212-668-2202...

  8. 75 FR 23843 - Discretionary Bus and Bus Facilities Program

    Science.gov (United States)

    2010-05-04

    ... assistance to subrecipients that are public agencies, private companies engaged in public transportation, or... bus-related projects for assistance to subrecipients that are public agencies, private companies... projects for assistance to subrecipients that are public agencies, private companies engaged in public...

  9. Expressiveness of basic Splice

    NARCIS (Netherlands)

    J.C. van de Pol (Jaco)

    2000-01-01

    textabstractWe study a simple software architecture, in which application processes are coordinated by writing into and reading from a global set. This architecture underlies Splice, which is developed and used at the company Hollandse Signaalapparaten. Our approach is distinguished by viewing the

  10. An experimental investigation on contact compression lap splice in circular columns

    Directory of Open Access Journals (Sweden)

    Hamed S. Askar

    2016-08-01

    The objective of the present investigation is to study the influence of splice length, volume of transverse reinforcement and end bearing condition on the behavior of compression lap splice. The conducted investigation included experimental tests of nine circular columns under uniaxial compression loads. All spliced bars were in contact with each other and with constant concrete cover. Based on the experimental investigation and test results, concluding remarks have been drawn, based on which a design simplified equation for splice length in compression has been developed. A correlation between the experimental and calculated results of the author specimens and other results available in literature, showed a good agreement. Also, the formulas adapted by different codes for predicting the compression lap splice length have been checked with the proposed equation.

  11. HERMES travels by CAN bus

    Science.gov (United States)

    Waller, Lewis G.; Shortridge, Keith; Farrell, Tony J.; Vuong, Minh; Muller, Rolf; Sheinis, Andrew I.

    2014-07-01

    The new HERMES spectrograph represents the first foray by AAO into the use of commercial off-the-shelf industrial field bus technology for instrument control, and we regard the final system, with its relatively simple wiring requirements, as a great success. However, both software and hardware teams had to work together to solve a number of problems integrating the chosen CANopen/CAN bus system into our normal observing systems. A Linux system running in an industrial PC chassis ran the HERMES control software, using a PCI CAN bus interface connected to a number of distributed CANopen/CAN bus I/O devices and servo amplifiers. In the main, the servo amplifiers performed impressively, although some experimentation with homing algorithms was required, and we hit a significant hurdle when we discovered that we needed to disable some of the encoders used during observations; we learned a lot about how servo amplifiers respond when their encoders are turned off, and about how encoders react to losing power. The software was based around a commercial CANopen library from Copley Controls. Early worries about how this heavily multithreaded library would work with our standard data acquisition system led to the development of a very low-level CANopen software simulator to verify the design. This also enabled the software group to develop and test almost all the control software well in advance of the construction of the hardware. In the end, the instrument went from initial installation at the telescope to successful commissioning remarkably smoothly.

  12. Double-Barred Galaxies

    OpenAIRE

    Erwin, Peter

    2009-01-01

    I present a brief review of what is known about double-barred galaxies, where a small ("inner") bar is nested inside a larger ("outer") bar; the review is focused primarily on their demographics and photometric properties. Roughly 20% of S0--Sb galaxies are double-barred; they may be rarer in later Hubble types. Inner bars are typically ~ 500 pc in radius (~ 12% the size of outer bars), but sizes range from ~ 100 pc to > 1 kpc. The structure of at least some inner bars appears very similar to...

  13. Electrical system architecture having high voltage bus

    Science.gov (United States)

    Hoff, Brian Douglas [East Peoria, IL; Akasam, Sivaprasad [Peoria, IL

    2011-03-22

    An electrical system architecture is disclosed. The architecture has a power source configured to generate a first power, and a first bus configured to receive the first power from the power source. The architecture also has a converter configured to receive the first power from the first bus and convert the first power to a second power, wherein a voltage of the second power is greater than a voltage of the first power, and a second bus configured to receive the second power from the converter. The architecture further has a power storage device configured to receive the second power from the second bus and deliver the second power to the second bus, a propulsion motor configured to receive the second power from the second bus, and an accessory motor configured to receive the second power from the second bus.

  14. PinBus Interface Design

    Energy Technology Data Exchange (ETDEWEB)

    Hammerstrom, Donald J.; Adgerson, Jewel D.; Sastry, Chellury; Pratt, Richard M.; Pratt, Robert G.

    2009-12-30

    On behalf of the U.S. Department of Energy, PNNL has explored and expanded upon a simple control interface that might have merit for the inexpensive communication of smart grid operational objectives (demand response, for example) to small electric end-use devices and appliances. The approach relies on bi-directional communication via the electrical voltage states of from one to eight shared interconnection pins. The name PinBus has been suggested and adopted for the proposed interface protocol. The protocol is defined through the presentation of state diagrams and the pins’ functional definitions. Both simulations and laboratory demonstrations are being conducted to demonstrate the elegance and power of the suggested approach. PinBus supports a very high degree of interoperability across its interfaces, allowing innumerable pairings of devices and communication protocols and supporting the practice of practically any smart grid use case.

  15. Avionic Data Bus Integration Technology

    Science.gov (United States)

    1991-12-01

    address the hardware-software interaction between a digital data bus and an avionic system. Very Large Scale Integration (VLSI) ICs and multiversion ...the SCP. In 1984, the Sperry Corporation developed a fault tolerant system which employed multiversion programming, voting, and monitoring for error... MULTIVERSION PROGRAMMING. N-version programming. 226 N-VERSION PROGRAMMING. The independent coding of a number, N, of redundant computer programs that

  16. New TPG bus route 28

    CERN Multimedia

    2003-01-01

    Geneva's Public Transport services (TPG) have recently inaugurated a new bus line 28, connecting the La Tour Hospital in Meyrin to the international organisations in Geneva, via the airport. All signs associated with this route will be yellow in colour. Timetables and route details can be found at http://www.tpg.ch. Relations with the Host States Service http://www.cern.ch/relations/ Tel. 72848

  17. Mutual interdependence of splicing and transcription elongation.

    Science.gov (United States)

    Brzyżek, Grzegorz; Świeżewski, Szymon

    2015-01-01

    Transcription and splicing are intrinsically linked, as splicing needs a pre-mRNA substrate to commence. The more nuanced view is that the rate of transcription contributes to splicing regulation. On the other hand there is accumulating evidence that splicing has an active role in controlling transcription elongation by DNA-dependent RNA polymerase II (RNAP II). We briefly review those mechanisms and propose a unifying model where splicing controls transcription elongation to provide an optimal timing for successive rounds of splicing.

  18. Barred Owl [ds8

    Data.gov (United States)

    California Department of Resources — These data define the current range of Barred and hybrid Barred/Spotted Owls in California. The current range includes the coastal mountains of northern California...

  19. 76 FR 78732 - FY 2011 Discretionary Funding Opportunity; Section 5309 Bus and Bus Facilities Veterans...

    Science.gov (United States)

    2011-12-19

    ... Administration's priority of supporting America's veterans and military families, as well as the objectives of... Federal Transit Administration FY 2011 Discretionary Funding Opportunity; Section 5309 Bus and Bus Facilities Veterans Transportation and Community Living Initiative AGENCY: Federal Transit Administration...

  20. National fuel cell bus program : proterra fuel cell hybrid bus report, Columbia demonstration.

    Science.gov (United States)

    2011-10-01

    This report summarizes the experience and early results from a fuel cell bus demonstration funded by the Federal Transit Administration (FTA) under the National Fuel Cell Bus Program. A team led by the Center for Transportation and the Environment an...

  1. Bus transport in Greater Manchester

    DEFF Research Database (Denmark)

    Sørensen, Claus Hedegaard; Gudmundsson, Henrik

    is that ‘organisation matters’ and that the reforms may have changed significantly the institutional conditions for the implementation of sustainable transport policies and easures. We have focused the research on public transport in larger cities and how their organisation has changed due to the reforms. At the NECTAR......, and will be elaborated by the time of the NECTAR workshop in May: • Deregulation and privatisation have not changed negative trends in modal split in Greater Manchester, but likely made it worse at least compared to London. • Deregulation and privatisation have worsened conditions for a greening of the bus fleets...

  2. American fuel cell bus project : first analysis report.

    Science.gov (United States)

    2013-06-01

    This report summarizes the experience and early results from the American Fuel Cell Bus Project, a fuel cell electric bus demonstration : funded by the Federal Transit Administration (FTA) under the National Fuel Cell Bus Program. A team led by CALST...

  3. Road safety issues for bus transport management.

    Science.gov (United States)

    Cafiso, Salvatore; Di Graziano, Alessandro; Pappalardo, Giuseppina

    2013-11-01

    Because of the low percentage of crashes involving buses and the assumption that public transport improves road safety by reducing vehicular traffic, public interest in bus safety is not as great as that in the safety of other types of vehicles. It is possible that less attention is paid to the significance of crashes involving buses because the safety level of bus systems is considered to be adequate. The purpose of this study was to evaluate the knowledge and perceptions of bus managers with respect to safety issues and the potential effectiveness of various technologies in achieving higher safety standards. Bus managers were asked to give their opinions on safety issues related to drivers (training, skills, performance evaluation and behaviour), vehicles (maintenance and advanced devices) and roads (road and traffic safety issues) in response to a research survey. Kendall's algorithm was used to evaluate the level of concordance. The results showed that the majority of the proposed items were considered to have great potential for improving bus safety. The data indicated that in the experience of the participants, passenger unloading and pedestrians crossing near bus stops are the most dangerous actions with respect to vulnerable users. The final results of the investigation showed that start inhibition, automatic door opening, and the materials and internal architecture of buses were considered the items most strongly related to bus passenger safety. Brake assistance and vehicle monitoring systems were also considered to be very effective. With the exception of driver assistance systems for passenger and pedestrian safety, the perceptions of the importance of other driver assistance systems for vehicle monitoring and bus safety were not unanimous among the bus company managers who participated in this survey. The study results showed that the introduction of new technologies is perceived as an important factor in improving bus safety, but a better understanding

  4. Intronic Alus influence alternative splicing.

    Directory of Open Access Journals (Sweden)

    Galit Lev-Maor

    2008-09-01

    Full Text Available Examination of the human transcriptome reveals higher levels of RNA editing than in any other organism tested to date. This is indicative of extensive double-stranded RNA (dsRNA formation within the human transcriptome. Most of the editing sites are located in the primate-specific retrotransposed element called Alu. A large fraction of Alus are found in intronic sequences, implying extensive Alu-Alu dsRNA formation in mRNA precursors. Yet, the effect of these intronic Alus on splicing of the flanking exons is largely unknown. Here, we show that more Alus flank alternatively spliced exons than constitutively spliced ones; this is especially notable for those exons that have changed their mode of splicing from constitutive to alternative during human evolution. This implies that Alu insertions may change the mode of splicing of the flanking exons. Indeed, we demonstrate experimentally that two Alu elements that were inserted into an intron in opposite orientation undergo base-pairing, as evident by RNA editing, and affect the splicing patterns of a downstream exon, shifting it from constitutive to alternative. Our results indicate the importance of intronic Alus in influencing the splicing of flanking exons, further emphasizing the role of Alus in shaping of the human transcriptome.

  5. Mastering NServiceBus and persistence

    CERN Document Server

    Helton, Rich

    2014-01-01

    This book is intended for developers, designers, and architects alike who wish to build C# NServiceBus enterprise architectures and learn how ESB persists data and messages to help them attain their goals. No prior knowledge of persistence in NServiceBus is required.

  6. Handbook of knotting and splicing

    CERN Document Server

    Hasluck, Paul N

    2005-01-01

    Clearly written and amply illustrated with 208 figures, this classic guide ranges from simple and useful knots to complex varieties. Additional topics include rope splicing, working cordage, hammock making, more.

  7. Methods to detect faulty splices in the superconducting magnet system of the LHC

    CERN Document Server

    Bailey, R; Catalan Lasheras, N; Dahlerup-Petersen, K; Denz, R; Robles, C; Koratzinos, M; Pojer, M; Ponce, L; Saban, R; Schmidt, R; Siemko, A; Solfaroli Camillocci, M; Thiesen, H; Vergara Fernandez, A; Flora, R H; Charifoulline, Z; Bednarek, M; Górnicki, E; Jurkiewicz, P; Kapusta, P; Strait, J

    2010-01-01

    The incident of 19 September 2008 at the LHC was caused by a faulty inter-magnet splice of about 200 nΩ resistance. Cryogenic and electrical techniques have been developed to detect other abnormal splices, either between or inside the magnets. The existing quench protection system can be used to detect internal splices with R>20 nΩ. Since this system does not cover the bus between magnets, the cryogenic system is used to measure the rate of temperature rise due to ohmic heating. Accuracy of a few mK/h, corresponding to a few Watts, has been achieved, allowing detection of excess resistance, if it is more than 40 nΩ in a cryogenic subsector (two optical cells). Follow-up electrical measurements are made in regions identified by the cryogenic system. These techniques have detected two abnormal internal magnet splices of 100 nΩ and 50 nΩ respectively. In 2009, this ad hoc system will be replaced with a permanent one to monitor all splices at the nΩ level.

  8. Tow Bar Test Plan

    Science.gov (United States)

    2011-06-22

    rounded edges to simulate a female bare human hand, assuming a one-hand bar design. If mechanical assist aids (i.e. narrow hook UNCLASSIFIED...UNCLASSIFIED for hoisting ) will be used to pick up the tow bar from the grab handles, the more severe load application shall be tested. (Reference... Hook Up Compatibility: Using the -10 operator manuals as guidance and the validated tow bars from step 12 of the laboratory testing, perform the

  9. Bar and Theta Hyperoperations

    Directory of Open Access Journals (Sweden)

    Thomas Vougiouklis

    2011-12-01

    Full Text Available In questionnaires the replacement of the scale of Likert by a bar was suggested in 2008 by Vougiouklis & Vougiouklis. The use of the bar was rapidly accepted in social sciences. The bar is closely related with fuzzy theory and has several advantages during both the filling-in questionnaires and mainly in the research processing. In this paper we relate hyperstructure theory with questionnaires and we study the obtained hyperstructures which are used as an organising device of the problem.

  10. Lightweight Composite Tow Bar

    Science.gov (United States)

    1981-06-01

    Flexural Property ASTM D740 Horizontal Shear ASTM D2344 Tensile Properties ASTM D3039 Compression Properties ASTM D3410 The data from the material tests...8E It M eeeay idenftfty by block nnmber) This program was initiated to develop a lightweight military standard heavy duty tow bar manufactured from...organic composites. A tow bar weighing 125 lbs (vs 340 lbs for standard tow bar) was successfully designed and fabricated from graphite reinforced epoxy

  11. Natural gas powered bus; Erdgasbusprojekt

    Energy Technology Data Exchange (ETDEWEB)

    Ambuehl, D.; Fernandez, J.

    2003-07-01

    This report for the Swiss Federal Office of Energy presents the results of a project carried out by the Swiss Federal Institute of Technology in Zurich to evaluate the performance of a natural-gas-powered bus in comparison with two diesel buses. The report provides details on the vehicles, their routes and the results of interviews made with both passengers and drivers. Details of measurements made on fuel consumption and pollutant emissions are presented in tabular and graphical form, as are those made on noise emissions inside and outside the vehicles. The conclusions of the project are presented including economic aspects of using gas as a motor fuel. Also, the views of passengers, who were more concerned with comfort aspects, and drivers, who were more interested in technical aspects, are quoted.

  12. Bus and Tag Terminators for IBM system/360

    CERN Multimedia

    Control units were connected to the channels with "Bus and Tag" cable pairs. The bus cables carried the address and data information and the tag cables identified what data was on the bus. There were three general types of bus-and-tag cables produced by IBM.

  13. Raising the bar (5)

    NARCIS (Netherlands)

    Elhorst, P.; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Yu, J.

    Raising the bar (5). Spatial Economic Analysis. This editorial summarizes and comments on the papers published in this issue 12(1) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper examines the impact of the level of education on the decision to

  14. Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors.

    Science.gov (United States)

    Wang, Yang; Wang, Zefeng

    2016-01-01

    The majority of human genes undergo alternative splicing to produce multiple isoforms with distinct functions. The dysregulations of alternative splicing have been found to be closely associated with various human diseases; thus new approaches to modulate disease-associated splicing events will provide great therapeutic potentials. Here we report protocols for constructing novel artificial splicing factors that can be designed to specifically modulate alternative splicing of target genes. By following the method outlined in this protocol, it is possible to design and generate artificial splicing factors with diverse activities in regulating different types of alternative splicing. The artificial splicing factors can be used to change splicing of either minigenes or endogenous genes in cultured human cells, providing a new strategy to study the regulation of alternative splicing and function of alternatively spliced products.

  15. Bus operator perceptions of safety risks.

    Science.gov (United States)

    2013-07-01

    This paper presents the results of a survey of TriMet bus operators addressing safety risks in their assigned work. Surveyed risk factors : were organized into five categories: vehicle design and condition; route layout; operating conditions; fatigue...

  16. MPBUS: Modular Power bus for Space Vehicles

    OpenAIRE

    Martin Ricardo; Remírez Eduardo; Alonso Javier; Priel Eran; Carapelle Alain; Actis Daniel

    2017-01-01

    Cost reduction in the access to space is leading the space race in the launchers market. Launcher’s companies are looking for a more efficient and economical way to place satellites in orbit. MPBUS project proposes a decentralized power bus distribution with jettisonable smart batteries combined with the use of PLC technology, data over DC, achieving overall weight reduction and harness simplification, both goals in line with Next Generation Launchers (NGL) targets. Two different bus in...

  17. ISET ORS Bus Standards and Prototype

    OpenAIRE

    Sandhoo, Gurpartap; Johnson, Mark; Raynor, William; Hurley, Michael; Stadter, Patrick; Marley, Michael; Apland, Clint; Lee, Robert; Bruzzi, Jonathan; Williams, Bruce; SCHAEFER, EDWARD, JAY; Vernon, Steven; Schwartz, Paul; Moretti, George; Doyne, Thomas

    2007-01-01

    Advancing sound and accepted spacecraft bus standards is the objective of the Office of the Secretary of Defense’s (OSD) Operationally Responsive Space (ORS) Bus Standards Initiative. This effort involves multiple government, industry, and academia participants assembled into an Integrated System Engineering Team (ISET). The core ISET industry team members include AeroAstro, Boeing, Design Net Engineering, General Dynamics Spectrum Astro, Loral, Microcosm, MicroSat, Orbital, Raytheon, and Swa...

  18. Experimental determinations of the eigenmodes for composite bars made with carbon and Kevlar-carbon fibers

    Science.gov (United States)

    Miriţoiu, C. M.; Stănescu, M. M.; Burada, C. O.; Bolcu, D.; Roşca, V.

    2015-11-01

    For modal identification, the single-point excitation method has been widely used in modal tests and it consists in applying a force in a given point and recording the vibratory structure response in all interest points, including the excitation point. There will be presented the experimental recordings for the studied bars (with Kevlar-carbon or carbon fibers), the frequency response function in Cartesian and polar coordinates. By using the frequency response functions we determine the eigenparameters for each bar. We present the final panel of the eigenmodes (with the damping factors, eigenfrequencies and critical damping) for each considered bar. Using the eigenfrequency of the first determined eigenmode, the bars stiffness has been determined. The presented bars can be used in practical engineering for: car or bus body parts, planes body parts, bullet-proof vests, reinforcements for sandwich beams, and so on.

  19. Splice testing for LHC quadrupole magnets

    CERN Document Server

    Barzi, E; Fehér, S; Kashikhin, V V; Kerby, J S; Lamm, M J; Orris, D; Ray, G; Tartaglia, M; Zlobin, A V

    2003-01-01

    Electrical splices between NbTi Rutherford type cables need to be made for the LHC IR inner triplet quadrupoles. Splices between magnets as well as internal to the magnets are necessary. Various splice configurations, solders, and fluxes have been considered. Testing of these splices at cryogenic temperatures and at various currents has been completed. The results were satisfactory; Fermilab is capable of making excellent low resistance (<1n Omega ) solder joints for the LHC project. (4 refs).

  20. Security awareness for public bus transportation : case studies of attacks against the Israeli public bus system.

    Science.gov (United States)

    2012-03-01

    This report presents 16 case studies of attacks planned or carried out against Israeli bus targets, along with statistical data on the number, frequency, and lethality of attacks against bus targets that have taken place in Israel since 1970 and duri...

  1. Bullying and Aggression on the School Bus: School Bus Drivers' Observations and Suggestions

    Science.gov (United States)

    deLara, Ellen

    2008-01-01

    Every school day bus drivers are responsible for transporting children safely over many miles, yet they are rarely polled for their opinions or contributions to school safety. School bus drivers are in a unique position to inform the discussion on aggressive behavior during the school day. This exploratory study collected information from school…

  2. Dynamic Bus Travel Time Prediction Models on Road with Multiple Bus Routes.

    Science.gov (United States)

    Bai, Cong; Peng, Zhong-Ren; Lu, Qing-Chang; Sun, Jian

    2015-01-01

    Accurate and real-time travel time information for buses can help passengers better plan their trips and minimize waiting times. A dynamic travel time prediction model for buses addressing the cases on road with multiple bus routes is proposed in this paper, based on support vector machines (SVMs) and Kalman filtering-based algorithm. In the proposed model, the well-trained SVM model predicts the baseline bus travel times from the historical bus trip data; the Kalman filtering-based dynamic algorithm can adjust bus travel times with the latest bus operation information and the estimated baseline travel times. The performance of the proposed dynamic model is validated with the real-world data on road with multiple bus routes in Shenzhen, China. The results show that the proposed dynamic model is feasible and applicable for bus travel time prediction and has the best prediction performance among all the five models proposed in the study in terms of prediction accuracy on road with multiple bus routes.

  3. Statistical Analysis of Bus Networks in India.

    Science.gov (United States)

    Chatterjee, Atanu; Manohar, Manju; Ramadurai, Gitakrishnan

    2016-01-01

    In this paper, we model the bus networks of six major Indian cities as graphs in L-space, and evaluate their various statistical properties. While airline and railway networks have been extensively studied, a comprehensive study on the structure and growth of bus networks is lacking. In India, where bus transport plays an important role in day-to-day commutation, it is of significant interest to analyze its topological structure and answer basic questions on its evolution, growth, robustness and resiliency. Although the common feature of small-world property is observed, our analysis reveals a wide spectrum of network topologies arising due to significant variation in the degree-distribution patterns in the networks. We also observe that these networks although, robust and resilient to random attacks are particularly degree-sensitive. Unlike real-world networks, such as Internet, WWW and airline, that are virtual, bus networks are physically constrained. Our findings therefore, throw light on the evolution of such geographically and constrained networks that will help us in designing more efficient bus networks in the future.

  4. GC content around splice sites affects splicing through pre-mRNA secondary structures

    Directory of Open Access Journals (Sweden)

    Chen Liang

    2011-01-01

    Full Text Available Abstract Background Alternative splicing increases protein diversity by generating multiple transcript isoforms from a single gene through different combinations of exons or through different selections of splice sites. It has been reported that RNA secondary structures are involved in alternative splicing. Here we perform a genomic study of RNA secondary structures around splice sites in humans (Homo sapiens, mice (Mus musculus, fruit flies (Drosophila melanogaster, and nematodes (Caenorhabditis elegans to further investigate this phenomenon. Results We observe that GC content around splice sites is closely associated with the splice site usage in multiple species. RNA secondary structure is the possible explanation, because the structural stability difference among alternative splice sites, constitutive splice sites, and skipped splice sites can be explained by the GC content difference. Alternative splice sites tend to be GC-enriched and exhibit more stable RNA secondary structures in all of the considered species. In humans and mice, splice sites of first exons and long exons tend to be GC-enriched and hence form more stable structures, indicating the special role of RNA secondary structures in promoter proximal splicing events and the splicing of long exons. In addition, GC-enriched exon-intron junctions tend to be overrepresented in tissue-specific alternative splice sites, indicating the functional consequence of the GC effect. Compared with regions far from splice sites and decoy splice sites, real splice sites are GC-enriched. We also found that the GC-content effect is much stronger than the nucleotide-order effect to form stable secondary structures. Conclusion All of these results indicate that GC content is related to splice site usage and it may mediate the splicing process through RNA secondary structures.

  5. Titin Diversity—Alternative Splicing Gone Wild

    Directory of Open Access Journals (Sweden)

    Wei Guo

    2010-01-01

    Full Text Available Titin is an extremely large protein found in highest concentrations in heart and skeletal muscle. The single mammalian gene is expressed in multiple isoforms as a result of alternative splicing. Although titin isoform expression is controlled developmentally and in a tissue specific manner, the vast number of potential splicing pathways far exceeds those described in any other alternatively spliced gene. Over 1 million human splice pathways for a single individual can be potentially derived from the PEVK region alone. A new splicing pattern for the human cardiac N2BA isoform type has been found in which the PEVK region includes only the N2B type exons. The alterations in splicing and titin isoform expression in human heart disease provide impetus for future detailed study of the splicing mechanisms for this giant protein.

  6. Statistical Analysis of Bus Networks in India

    CERN Document Server

    Chatterjee, Atanu; Ramadurai, Gitakrishnan

    2015-01-01

    Through the past decade the field of network science has established itself as a common ground for the cross-fertilization of exciting inter-disciplinary studies which has motivated researchers to model almost every physical system as an interacting network consisting of nodes and links. Although public transport networks such as airline and railway networks have been extensively studied, the status of bus networks still remains in obscurity. In developing countries like India, where bus networks play an important role in day-to-day commutation, it is of significant interest to analyze its topological structure and answer some of the basic questions on its evolution, growth, robustness and resiliency. In this paper, we model the bus networks of major Indian cities as graphs in \\textit{L}-space, and evaluate their various statistical properties using concepts from network science. Our analysis reveals a wide spectrum of network topology with the common underlying feature of small-world property. We observe tha...

  7. The Bus Station Spacing Optimization Based on Game Theory

    Directory of Open Access Journals (Sweden)

    Changjiang Zheng

    2015-01-01

    Full Text Available With the development of city, the problem of traffic is becoming more and more serious. Developing public transportation has become the key to solving this problem in all countries. Based on the existing public transit network, how to improve the bus operation efficiency, and reduce the residents transit trip cost has become a simple and effective way to develop the public transportation. Bus stop spacing is an important factor affecting passengers’ travel time. How to set up bus stop spacing has become the key to reducing passengers’ travel time. According to comprehensive traffic survey, theoretical analysis, and summary of urban public transport characteristics, this paper analyzes the impact of bus stop spacing on passenger in-bus time cost and out-bus time cost and establishes in-bus time and out-bus time model. Finally, the paper gets the balance best station spacing by introducing the game theory.

  8. Stress prevention in bus drivers: Evaluation of 13 natural experiments

    NARCIS (Netherlands)

    Kompier, M.A.J.; Aust, B.; Berg, A.M. van den; Siegrist, J.

    2000-01-01

    The research aim was to select, compare, and analyze interventions and preventive actions from international bus companies to decrease bus drivers' occupational stress and sickness absenteeism. Through networking, international surveys, and literature study, 13 "natural experiments" were identified

  9. Request-Centric Wireless Bus Information Management System

    Directory of Open Access Journals (Sweden)

    Ying-Chih Chen

    2016-11-01

    Full Text Available This invention relates to a wireless bus information management system, which includes bus stop and vehicle management subsystems. The management signals are transmittable between bus stops and the vehicle. Based on vehicle management signals, the bus stop management subsystem can obtain information about the bus route identification, the number of unoccupied seats, the intention to stop or not, etc. Similarly, with the bus stop management signals, the vehicle management subsystem can make the decision of stopping. Accordingly, when a passenger wants to get off the vehicle or there are unoccupied seats, the vehicle management subsystem will inform the bus stop management subsystem such that the passengers waiting at the bus stop may flexibly schedule their travel plan. The proposed distributed wireless system is detailed by a prototype implementation and a simulation analysis, which is shown to be feasible and scalable.

  10. Splicing and alternative splicing in rice and humans

    Directory of Open Access Journals (Sweden)

    Zhiguo E

    2013-09-01

    Full Text Available Rice is a monocot gramineous crop, and one of the mostimportant staple foods. Rice is considered a model species formost gramineous crops. Extensive research on rice hasprovided critical guidance for other crops, such as maize andwheat. In recent years, climate change and exacerbated soildegradation have resulted in a variety of abiotic stresses, suchas greenhouse effects, lower temperatures, drought, floods,soil salinization and heavy metal pollution. As such, there isan extremely high demand for additional research, in order toaddress these negative factors. Studies have shown that thealternative splicing of many genes in rice is affected by stressconditions, suggesting that manipulation of the alternativesplicing of specific genes may be an effective approach for riceto adapt to abiotic stress. With the advancement ofmicroarrays, and more recently, next generation sequencingtechnology, several studies have shown that more than half ofthe genes in the rice genome undergo alternative splicing. Thismini-review summarizes the latest progress in the research ofsplicing and alternative splicing in rice, compared to splicingin humans. Furthermore, we discuss how additional studiesmay change the landscape of investigation of rice functionalgenomics and genetically improved rice. [BMB Reports 2013;46(9: 439-447

  11. Analysis of a diesel-electric hybrid urban bus system

    Science.gov (United States)

    Marr, W. W.; Sekar, R. R.; Ahlheim, M. C.

    A hybrid bus powered by a diesel engine and a battery pack was analyzed over an idealized bus-driving cycle in Chicago. Three hybrid configurations, two parallel and one series, were evaluated. The results indicate that the fuel economy of a hybrid bus, taking into account the regenerative braking, is comparable with that of a conventional diesel bus. Life-cycle costs are slightly higher because of the added weight and cost of the battery.

  12. 45 CFR 1310.17 - Driver and bus monitor training.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Driver and bus monitor training. 1310.17 Section... START PROGRAM HEAD START TRANSPORTATION Transportation Requirements § 1310.17 Driver and bus monitor... performance; and (2) before bus monitors assigned to vehicles used to provide such services begin their duties...

  13. 77 FR 43646 - FY 2012 Discretionary Funding Opportunity; Section 5309 Bus and Bus Facilities and Section 5312...

    Science.gov (United States)

    2012-07-25

    ... Administration's priority of supporting America's veterans and military families, as well as the objectives of... Federal Transit Administration FY 2012 Discretionary Funding Opportunity; Section 5309 Bus and Bus... AGENCY: Federal Transit Administration (FTA), DOT. ACTION: FTA Veterans Transportation and Community...

  14. Security awareness for public bus transportation : case studies of attacks against the Israeli public bus system [research brief].

    Science.gov (United States)

    2012-03-01

    Government regulators and transportation operators of public bus systems are concerned about terrorist attacks. Bus attacks in Israel between late September 2000 and the end of 2006, a period known as the Second Intifada, are particularly well known....

  15. Safely splicing glass optical fibers

    Science.gov (United States)

    Korbelak, K.

    1980-01-01

    Field-repair technique fuses glass fibers in flammable environment. Apparatus consists of v-groove vacuum chucks on manipulators, high-voltage dc power supply and tungsten electrodes, microscope to observe joint alignment and fusion, means of test transmission through joint. Apparatus is enclosed in gas tight bos filled with inert gas during fusion. About 2 feet of fiber end are necessary for splicing.

  16. Technology Validation: Fuel Cell Bus Evaluations

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2018-01-02

    This presentation describing the FY 2016 accomplishments for the National Renewable Energy Laboratory's Fuel Cell Bus Evaluations project was presented at the U.S. Department of Energy Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting, June 7, 2016.

  17. FUEL CELL BUS DEMONSTRATION IN MEXICO CITY

    Science.gov (United States)

    The report discusses the performance of a cull-size, zero-emission, Proton Exchange Membrane (PEM) fuel-cell-powered transit bus in the atmospheric environment of Mexico City. To address the air quality problems caused by vehicle emissions in Mexico City, a seminar on clean vehic...

  18. An Experimental Platform for Autonomous Bus Development

    Directory of Open Access Journals (Sweden)

    Héctor Montes

    2017-11-01

    Full Text Available Nowadays, with highly developed instrumentation, sensing and actuation technologies, it is possible to foresee an important advance in the field of autonomous and/or semi-autonomous transportation systems. Intelligent Transport Systems (ITS have been subjected to very active research for many years, and Bus Rapid Transit (BRT is one area of major interest. Among the most promising transport infrastructures, the articulated bus is an interesting, low cost, high occupancy capacity and friendly option. In this paper, an experimental platform for research on the automatic control of an articulated bus is presented. The aim of the platform is to allow full experimentation in real conditions for testing technological developments and control algorithms. The experimental platform consists of a mobile component (a commercial articulated bus fully instrumented and a ground test area composed of asphalt roads inside the Consejo Superior de Investigaciones Científicas (CSIC premises. This paper focuses also on the development of a human machine interface to ease progress in control system evaluation. Some experimental results are presented in order to show the potential of the proposed platform.

  19. Rolling horizon predictions of bus trajectories

    NARCIS (Netherlands)

    Oshyani, M.F.; Cats, O.

    2014-01-01

    Bus travel times are subject to inherent and recurrent uncertainties. A real-time prediction scheme regarding how the transit system evolves will potentially facilitate more adaptive operations as well as more adaptive passengers’ decisions. This scheme should be tractable, sufficiently fast and

  20. Stochastic Bus Dispatching Model untuk Optimalisasi Jumlah Bus Transjakarta Koridor 3

    Directory of Open Access Journals (Sweden)

    Zahedi Zahedi

    2011-12-01

    Full Text Available The increasing number of road user causing traffic jam finds out a way, busway. However, in its implementation, the number of fleet available does not enough to take the passengers. It results in the an amassed of passengers at several bus stops. This leads to passenger dissatisfaction satisfaction which eventually causes Transjakarta users to reuse private cars. Definitely this situation may builds up traffic jams. Stochastic Bus Dispatching Model is used to simulate public transportation that operates regularly. 

  1. Bus 54 -- Where are you? A school bus intelligent information system

    Energy Technology Data Exchange (ETDEWEB)

    Truett, L.F.; Moore, S.; Tonn, B. [Oak Ridge National Lab., TN (United States); Conley, T. [GTE Wireless, Nashville, TN (United States)

    1998-07-01

    Although major accidents involving school buses are rare (only about 0.3% of all fatal crashes since 1986 are classified as school-bus-related), even minor accidents and breakdowns cause a great deal of parental anxiety. The objective of this research is to design an efficient, cost-effective, accurate, and secure system that will track individual school buses and communicate appropriate information to the school system`s central administration unit, to the school transportation administrator, and to parents of children on the bus. The greatest benefit of the proposed information system is that parents and officials can always know the location and condition (these conditions would vary depending on the needs of a particular school system) of the school buses. In case of an accident or mechanical problem, when emergency crews are needed, they can be dispatched almost immediately with a good understanding of the problem and the exact location of the bus. In addition to being able to track the bus while the child is on board, parents will be able to determine the location of their child`s bus prior to its arrival in the morning in order to prevent the child from needing to wait outside in inclement weather. The information available to parents can also be expanded to include maps of limited routes (e.g., snow routes). Basically, the Bus 54 concept consists of a bus component and a central data processing unit. Each bus will be outfitted with a global positioning satellite (GPS) device, a wireless communication device, and wireless data communication service. The central data processing unit will receive and process information from the buses and provide information access to parents and officials via an Internet link.

  2. Raising the bar (2)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    2016-01-01

    In this editorial we summarise and comment on the papers published in issue 11.2 so as to raise the bar in applied spatial economic research and highlight new trends. The first paper analyses which regions in Europe were resilient to the great Recession and which ones were not. The second and the

  3. Raising the Bar (3)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    This editorial summarizes and comments on the papers published in issue 11(3) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper proposes spatial and a-spatial indicators to describe the networks of airline companies around the world. The second

  4. Breaking through the Bar

    Science.gov (United States)

    Gray, Katti

    2011-01-01

    Howard University School of Law had a problem, and school officials knew it. Over a 20-year period, 40 percent of its graduates who took the Maryland bar exam failed it on their first try. During the next 24 months--the time frame required to determine its "eventual pass rate"--almost 90 percent of the students did pass. What they did…

  5. Behind No Bars.

    Science.gov (United States)

    Rudel, Charlotte, H.; Hayes, Margaret L.

    1990-01-01

    Describes the Behind No Bars program at the Cambria County Prison in Johnstown, Pennsylvania, which was funded by Head Start. Children visited with a jailed parent in pleasant, child-oriented areas. Parenting classes and family counseling were offered to all inmates who were parents of young children. (BB)

  6. The IPG BAR project

    NARCIS (Netherlands)

    Van Timmeren, A.; Stolk, E.; Kleijn, R.; Mentink, B.; Henriquez, L.; Van Niekerk, L.; Verheul, R.

    2013-01-01

    The booklets 'Making Patterns' and 'Using Patterns' describe two aspects of the the pattern method within the urban design process. The accompanying IPG BAR Pattern Library is a small pattern library of urban-airport symbiosis patterns. These documents are the result of the Interdisciplinary Project

  7. Advanced eGovernment Information Service Bus (eGov-Bus

    Directory of Open Access Journals (Sweden)

    2007-01-01

    Full Text Available The eGov-Bus project provides citizens and businesses with improved access to virtual public services, which are based on existing national eGovernment Web services and which support cross-border life events. Requirements and specific rules of these life events are considered, and personalization of user preferences is supported. eGov-Bus is based on adaptable process management technologies, allowing for virtual services which are dynamically combined from existing national eGovernment services. In this way, a comprehensive workflow process is set up, allowing for service-level agreements, an audit trail and explanation of the process to the end user. The eGov-Bus process engine operates on top of a virtual repository, providing a high-level semantic view of information retrieved from heterogeneous information sources, such as eGovernment Web services. Further, eGov-Bus relies on a security framework to ensure all high-level security requirements are met. The eGov-Bus architecture is business oriented, it focuses on Service Oriented Architecture (SOA concepts, asynchronously combining Web services and providing a Service Bus.

  8. SplicePlot: a utility for visualizing splicing quantitative trait loci.

    Science.gov (United States)

    Wu, Eric; Nance, Tracy; Montgomery, Stephen B

    2014-04-01

    RNA sequencing has provided unprecedented resolution of alternative splicing and splicing quantitative trait loci (sQTL). However, there are few tools available for visualizing the genotype-dependent effects of splicing at a population level. SplicePlot is a simple command line utility that produces intuitive visualization of sQTLs and their effects. SplicePlot takes mapped RNA sequencing reads in BAM format and genotype data in VCF format as input and outputs publication-quality Sashimi plots, hive plots and structure plots, enabling better investigation and understanding of the role of genetics on alternative splicing and transcript structure. Source code and detailed documentation are available at http://montgomerylab.stanford.edu/spliceplot/index.html under Resources and at Github. SplicePlot is implemented in Python and is supported on Linux and Mac OS. A VirtualBox virtual machine running Ubuntu with SplicePlot already installed is also available.

  9. Shaping the Arabidopsis Transcriptome through Alternative Splicing

    Directory of Open Access Journals (Sweden)

    Dorothee Staiger

    2015-01-01

    Full Text Available Alternative splicing is a molecular tool of the cell to generate more than one messenger RNA from the same gene. Through variable combinations of exons blueprints for different proteins are assembled from one and the same pre-messenger RNA, thus increasing the complexity of the proteome. Moreover, through alternative splicing different transcript variants with different stabilities and different regulatory motifs can be generated, leading to variation in the transcriptome. The importance of alternative splicing in plants has been increasingly recognized in the last decade. Alternative splicing has been found during abiotic and biotic stress and during development. Here, recent advancements in the understanding of alternative splicing in higher plants are presented. Mechanistic details and functional consequences of alternative splicing are discussed with a focus on the model plant Arabidopsis thaliana.

  10. Shuttle bus services quality assessment Tangerang Selatan toward smart city

    Science.gov (United States)

    Fassa, Ferdinand; Sitorus, Fredy Jhon Philip; Adikesuma, Tri Nugraha

    2017-11-01

    Around the world, shuttle bus operation played the significant role to accommodate transportation for commuting bus passengers. Shuttle Bus services in cities are provided by various bus agencies with kinds of own specific purposes. For instance, at Tangerang Selatan, Indonesia, it was said that shuttle bus In Trans Bintaro is run and operated by private bus companies hire by Bintaro developer. The aim of this research is to identify factors of satisfaction of shuttle bus service in Kota Tangerang Selatan, Indonesia. Several factors are used to analyze sums of 20 parameters performance indicators of Shuttle Bus. A face to face interview using a questionnaire (N=200) was used to collect data on October and March 2017. Likert and diagram Cartesian were used to model the all the parameters. This research succeeded in finding some categories of Shuttle bus service attributes such as accessibility, comfort, and safety. Users agreed that eight indicators in shuttle bus have the excellent achievement, while three indicators on performance remain low and should receive more attention especially punctuality of the bus.

  11. Energy efficiency of different bus subsystems in Belgrade public transport

    Directory of Open Access Journals (Sweden)

    Mišanović Slobodan M.

    2015-01-01

    Full Text Available Research in this paper comprised experimental determination of energy efficiency of different bus subsystems (diesel bus, trolleybus and fully electric bus on chosen public transport route in Belgrade. Experimental measuring of energy efficiency of each bus type has been done based on the analysis of parameters of vehicle driving cycles between stops. Results of this analysis were basis for development of theoretical simulation model of energy efficiency. The model was latter compared with the results of simulation done by "Solaris bus & Coach" company for the chosen electric bus route. Based on demonstrated simulation, characteristics of electric bus batteries were defined, the method and dynamic of their recharge was suggested, as well as choice for other aggregates for drive system and technical characteristics for the electric buses were suggested.

  12. Bus accident severity and passenger injury: evidence from Denmark

    DEFF Research Database (Denmark)

    Prato, Carlo Giacomo; Kaplan, Sigal

    2014-01-01

    examining occurrence of injury to bus passengers. Results Bus accident severity is positively related to (i) the involvement of vulnerable road users, (ii) high speed limits, (iii) night hours, (iv) elderly drivers of the third party involved, and (v) bus drivers and other drivers crossing in yellow or red...... light. Occurrence of injury to bus passengers is positively related to (i) the involvement of heavy vehicles, (ii) crossing intersections in yellow or red light, (iii) open areas, (iv) high speed limits, and (v) slippery road surface. Conclusions The findings of the current study provide a comprehensive......Purpose Bus safety is a concern not only in developing countries, but also in the U.S. and Europe. In Denmark, disentangling risk factors that are positively or negatively related to bus accident severity and injury occurrence to bus passengers can contribute to promote safety as an essential...

  13. Integer programming model for optimizing bus timetable using genetic algorithm

    Science.gov (United States)

    Wihartiko, F. D.; Buono, A.; Silalahi, B. P.

    2017-01-01

    Bus timetable gave an information for passengers to ensure the availability of bus services. Timetable optimal condition happened when bus trips frequency could adapt and suit with passenger demand. In the peak time, the number of bus trips would be larger than the off-peak time. If the number of bus trips were more frequent than the optimal condition, it would make a high operating cost for bus operator. Conversely, if the number of trip was less than optimal condition, it would make a bad quality service for passengers. In this paper, the bus timetabling problem would be solved by integer programming model with modified genetic algorithm. Modification was placed in the chromosomes design, initial population recovery technique, chromosomes reconstruction and chromosomes extermination on specific generation. The result of this model gave the optimal solution with accuracy 99.1%.

  14. Computational prediction of splicing regulatory elements shared by Tetrapoda organisms

    OpenAIRE

    Churbanov, Alexander; Vo?echovsk?, Igor; Hicks, Chindo

    2009-01-01

    Abstract Background Auxiliary splicing sequences play an important role in ensuring accurate and efficient splicing by promoting or repressing recognition of authentic splice sites. These cis-acting motifs have been termed splicing enhancers and silencers and are located both in introns and exons. They co-evolved into an intricate splicing code together with additional functional constraints, such as tissue-specific and alternative splicing patterns. We used orthologous exons extracted from t...

  15. Aberrant RNA splicing in cancer; expression changes and driver mutations of splicing factor genes.

    Science.gov (United States)

    Sveen, A; Kilpinen, S; Ruusulehto, A; Lothe, R A; Skotheim, R I

    2016-05-12

    Alternative splicing is a widespread process contributing to structural transcript variation and proteome diversity. In cancer, the splicing process is commonly disrupted, resulting in both functional and non-functional end-products. Cancer-specific splicing events are known to contribute to disease progression; however, the dysregulated splicing patterns found on a genome-wide scale have until recently been less well-studied. In this review, we provide an overview of aberrant RNA splicing and its regulation in cancer. We then focus on the executors of the splicing process. Based on a comprehensive catalog of splicing factor encoding genes and analyses of available gene expression and somatic mutation data, we identify cancer-associated patterns of dysregulation. Splicing factor genes are shown to be significantly differentially expressed between cancer and corresponding normal samples, and to have reduced inter-individual expression variation in cancer. Furthermore, we identify enrichment of predicted cancer-critical genes among the splicing factors. In addition to previously described oncogenic splicing factor genes, we propose 24 novel cancer-critical splicing factors predicted from somatic mutations.

  16. Exposure Assessment of Diesel Bus Emissions

    Directory of Open Access Journals (Sweden)

    Werner Hofmann

    2006-12-01

    Full Text Available The goal of this study was to measure ultrafine particle concentrations with diameters less than 1 μm emitted by diesel buses and to assess resulting human exposure levels. The study was conducted at the Woolloongabba Busway station in Brisbane, Australia in the winter months of 2002 during which temperature inversions frequently occurred. Most buses that utilize the station are fuelled by diesel, the exhaust of which contains a significant quantity of particle matter. Passengers waiting at the station are exposed to these particles emitted from the buses. During the course of this study, passenger census was conducted, based on video surveillance, yielding person-by-person waiting time data. Furthermore, a bus census revealed accurate information about the total number of diesel versus Compressed Natural Gas (CNG powered buses. Background (outside of the bus station and platform measurements of ultrafine particulate number size distributions were made to determine ambient aerosol concentrations. Particle number exposure concentration ranges from 10 and 40 to 60% of bus related exhaust fumes. This changes dramatically when considering the particle mass exposure concentration, where most passengers are exposed to about 50 to 80% of exhaust fumes. The obtained data can be very significant for comparison with similar work of this type because it is shown in previous studies that exhaust emissions causes cancer in laboratory animals. It was assumed that significant differences between platform and background distributions were due to bus emissions which, combined with passenger waiting times, yielded an estimate of passenger exposure to ultrafine particles from diesel buses. From an exposure point of view, the Busway station analyzed resembles a street canyon. Although the detected exhaust particle concentration at the outbound platform is found to be in the picogram range, exposure increases with the time passengers spend on the platform

  17. Terminals for Suburb Bus Transport in Bratislava

    Science.gov (United States)

    Schlosser, Tibor; Schlosser, Peter; Cápayová, Silvia; Hodáková, Dominika

    2017-10-01

    The main objective of this article is to describe the strategy for development of the public transport terminals in the city of Bratislava, Capital of Slovak Republic. The reason goes from the private operator Slovak Lines, who operates the suburb bus transport in the agglomeration of the city. For this operator was created a transport model, while placing emphasis on optimizing the compliance of suburban public transport with urban public transport in the city of Bratislava and evaluating the significance of the new Bus Station to be constructed at Mlynské Nivy – in a new down town centre of the city. The main issue is to ensure the best available offer of public transport (PT) to passengers in the Bratislava agglomeration. The subject of the study was oriented to specify and propose changes in the transport infrastructure and integrated public transport organisation on the area of the city in terms of the significant position of the new Mlynské Nivy Bus Station (MN BS), which is under preparation with realization in the year 2017.

  18. Brazilian hybrid electric fuel cell bus

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, P.E.V.; Carreira, E.S. [Coppe-Federal Univ. of Rio de Janeiro (Brazil). Hydrogen Lab.

    2010-07-01

    The first prototype of a hybrid electric fuel cell bus developed with Brazilian technology is unveiled. It is a 12 m urban-type, low-floor, air-conditioned bus that possesses three doors, air suspension, 29 seats and reversible wheelchair site. The bus body was built based on a double-deck type monoblock vehicle that is able to sustain important load on its roof. This allowed positioning of the type 3 hydrogen tanks and the low weight traction batteries on the roof of the vehicles without dynamic stabilization problems. A novel hybrid energy configuration was designed in such a way that the low-power (77 kWe) fuel cell works on steady-state operation mode, not responding directly to the traction motor load demand. The rate of kinetic energy regeneration upon breaking was optimized by the use of an electric hybrid system with predominance of batteries and also by utilizing supercapacitors. The electric-electronic devices and the security control softwares for the auxiliary and traction systems were developed in-house. The innovative hybrid-electric traction system configuration led to the possibility to decrease the fuel cell power, with positive impact on weight and system volume reduction, as well as to significantly decrease the hydrogen consumption. (orig.)

  19. Research of intelligent bus coin box

    Science.gov (United States)

    Xin, Shihao

    2017-03-01

    In the energy-saving emission reduction of the social context, in response to low-carbon travel, buses become the majority of people choose. We have designed this sorting machine for the present situation that the bus company has received a large amount of mixed zero coins and employed a large amount of manpower to sort out and lower the efficiency. Its function is to separate the coins and notes mixed, and the coins sort storage, the display shows the value of the received coins, so that the whole mechanized inventory classification, reduce the cost of clearing up and improve the efficiency of zero cash recycling, use Simple mechanical principles for classification, to be efficient, accurate and practical. Really meet the current city bus companies, commerce and banking and other industries in order to zero notes, zero coins in the actual demand. The size and specification of this machine are designed according to the size of the bus coin box. It is suitable for almost all buses. It can be installed in the coin box directly, real-time sorting and real-time counting. The difficulty of clearing change.

  20. Development of A Maintenance Device for Bus-bar PT Voltage Air Switch

    Directory of Open Access Journals (Sweden)

    Zhang Xiang

    2017-01-01

    Full Text Available When PT breaks down, it takes long time of switching operation before maintenance, which seriously delays the restoration time. Based on the principle of multiple circuit, a live replacement maintenance device for PT voltage air switch is proposed. The following aspects are involved in the design of the device: the principle of device, component selection, device’s assembly and operation process. Through functional test in simulation substation and on-site installation, it is proved that the failed air switch can be lively replaced by the device without switching operation, which greatly reduces the risk on power grid caused by such faulted air switch.

  1. Quench propagation and detection in the superconducting bus-bars of the ATLAS magnets

    CERN Document Server

    Dudarev, A; ten Kate, H H J; Sbrissa, E; Yamamoto, A; Baynham, D Elwyn; Courthold, M J D; Lesmond, C

    2000-01-01

    The ATLAS superconducting magnet system comprising Barrel (BT) and End-Cap Toroids (ECT) and also Central Solenoid (CS) will store more than 1.5 GJ of magnetic energy. The magnet system will have many superconducting busbars, a few meters long each, running from the current leads to Central Solenoid and Toroids as well as between the coils of each Toroid. Quench development in the busbars, i.e., the normal zone propagation process along the busbar superconductors, is slow and exhibits very low voltages. Therefore, its timely and appropriate detection represents a real challenge. The temperature evolution in the busbars under quench is of primary importance. Conservative calculations of the temperature were performed for all the magnets. Also, a simple and effective method to detect a normal zone in a busbar is presented. A thin superconducting wire, whose normal resistance can be easily detected, is placed in a good thermal contact to busbar. Thus, the wire can operate as straightforward and low-noise quench-...

  2. Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay.

    Science.gov (United States)

    Movassat, Maliheh; Shenasa, Hossein; Hertel, Klemens J

    2017-01-01

    The ability to perform in vitro splicing assays has paved the way for in-depth studies of the mechanisms and machinery involved in the process of splicing. The in vitro splicing assay is a valuable experimental approach that combines the complexity of the spliceosome and regulatory systems with the flexibility of performing endless splicing and alternative splicing reactions. Through the use of crude nuclear extract and radiolabeled pre-mRNA, spliced mRNAs can be visualized using autoradiography for downstream analysis. This chapter describes the necessary steps to perform an in vitro splicing reaction, including the generation of the key components necessary for the splicing reaction; nuclear extract.

  3. Spliced

    DEFF Research Database (Denmark)

    Addison, Courtney Page

    2017-01-01

    been initiated. In this article I present a brief history of HGT, showing how the ethical and practical viability of the field was achieved by key scientific and regulatory actors. These parties carefully articulated gene therapy’s scope, limiting it to therapeutic interventions on somatic cells......, and cultivated alliances and divisions that bolstered the field’s legitimacy. At times these measures faltered, and then practitioners and sometimes patients would invoke an ethical imperative, posing gene therapy as the best solution to life and death problems. I suggest that we consider how boundary...

  4. Peptic ulcer among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1990-01-01

    As part of a survey on the work environment of bus drivers, 2045 (83%) of 2465 male bus drivers in the three major cities in Denmark in 1978 answered a postal questionnaire on health and working conditions. In order to evaluate the relative occurrence of peptic ulcer among the bus drivers, a follow......-up study was also conducted. All hospital discharges with a peptic ulcer diagnosis among the bus drivers were registered from the Danish National Patient Register. All Danish men were used as reference group. On the basis of the 1978-questionnaire association between occupational and psychosocial factors...... and subsequent hospital discharge with a peptic ulcer diagnosis was studied. The prevalence of abdominal pain alleviated by food intake was 12% among bus drivers and 6% in the reference group. The incidence of hospital discharge with duodenal ulcer among younger bus drivers was twice the incidence among Danish...

  5. Flexi-Bar-harjoittelu

    OpenAIRE

    Männikkö, Anu

    2007-01-01

    Opinnäytetyön tavoitteena on testata uuden harjoitteluvälineen, Flexi-Barin, merkitystä lantionpohjan lihasten maksimi-, nopeus- ja kestävyysvoimaan ja näin ollen löytää uusi harjoitteluväline lantionpohjan lihasten harjoittamiseen. Tarkoituksena on selvittää, miten lantionpohjan lihasten maksimi-, nopeus- ja kestävyysvoimat muuttuvat kahdeksan viikon Flexi-Bar-harjoittelun aikana. Syvien lihasten aktivointiin ja stabiloivaan vaikutukseen kiinnitetään terapeuttisessa harjoittelussa nyky...

  6. Ukola Club. Bar americano

    Directory of Open Access Journals (Sweden)

    Azpiazu, J. R.

    1961-03-01

    Full Text Available En la calle de Serrano, aprovechando un semisótano dedicado a otro negocio anteriormente, se ha instalado un bar americano, de cuyo interior ofrecemos algunos pormenores. Se han cuidado, especialmente, las condiciones acústicas, resueltas por medio de un techo de escayola perforada, con vitrofib en su parte superior, y paredes de madera, que contribuyen a darle un ambiente cálido y acogedor. El soporte de hierro laminado existente en el centro del local, cuya supresión hubiera sido costosa, se ha revestido con lajas de mármol que le convierten en un elemento decorativo.

  7. An Interspecific Plant Hybrid Shows Novel Changes in Parental Splice Forms of Genes for Splicing Factors

    Science.gov (United States)

    Scascitelli, Moira; Cognet, Marie; Adams, Keith L.

    2010-01-01

    Interspecific hybridization plays an important role in plant adaptive evolution and speciation, and the process often results in phenotypic novelty. Hybrids can show changes in genome structure and gene expression compared with their parents including chromosomal rearrangments, changes in cytosine methylation, up- and downregulation of gene expression, and gene silencing. Alternative splicing (AS) is a fundamental aspect of the expression of many genes. However alternative splicing patterns have not been examined in multiple genes in an interspecific plant hybrid compared with its parents. Here we studied alternative splicing patterns in an interspecific Populus hybrid and its parents by assaying 40 genes using reverse transcription PCR. Most of the genes showed identical alternative splicing patterns between the parents and the hybrid. We found new alternative splicing variants present in the hybrid in two SR genes involved in the regulation of splicing and alternative splicing. The novel alternative splicing patterns included changes in donor and acceptor sites to create a new exon in one allele of PtRSZ22 in the hybrid and retention of an intron in both alleles of PtSR34a.1 in the hybrid, with effects on the function of the corresponding truncated proteins, if present. Our results suggest that novel alternative splicing patterns are present in a small percentage of genes in hybrids, but they could make a considerable impact on the expression of some genes. Changes in alternative splicing are likely to be an important component of the genetic changes that occur upon interspecific hybridization. PMID:20100939

  8. Aberrant Splicing in Cancer: Mediators of Malignant Progression through an Imperfect Splice Program Shift.

    Science.gov (United States)

    Luz, Felipe Andrés Cordero; Brígido, Paula Cristina; Moraes, Alberto Silva; Silva, Marcelo José Barbosa

    2017-01-01

    Although the efforts to understand the genetic basis of cancer allowed advances in diagnosis and therapy, little is known about other molecular bases. Splicing is a key event in gene expression, controlling the excision of introns decoded inside genes and being responsible for 80% of the proteome amplification through events of alternative splicing. Growing data from the last decade point to deregulation of splicing events as crucial in carcinogenesis and tumor progression. Several alterations in splicing events were observed in cancer, caused by either missexpression of or detrimental mutations in some splicing factors, and appear to be critical in carcinogenesis and key events during tumor progression. Notwithstanding, it is difficult to determine whether it is a cause or consequence of cancer and/or tumorigenesis. Most reviews focus on the generated isoforms of deregulated splicing pattern, while others mainly summarize deregulated splicing factors observed in cancer. In this review, events associated with carcinogenesis and tumor progression mainly, and epithelial-to-mesenchymal transition, which is also implicated in alternative splicing regulation, will be progressively discussed in the light of a new perspective, suggesting that splicing deregulation mediates cell reprogramming in tumor progression by an imperfect shift of the splice program. © 2016 S. Karger AG, Basel.

  9. Design of Mine Locomotive System Based on CAN Bus

    OpenAIRE

    Li Yuanhong; Zhang Quanzhu; Zhang Wenshan

    2017-01-01

    Based on CAN bus, this paper studies the system control and management system of locomotive in mine, analyzes the working principle of locomotive system, gives the CAN bus scheme, hardware circuit design and CAN communication protocol, and implements long-distance, high-reliability communication function and remote monitoring function. Experiments show that the auxiliary system based on CAN bus control easier, operation more secure, as well as improving the control performance and service lif...

  10. Design of Mine Locomotive System Based on CAN Bus

    Directory of Open Access Journals (Sweden)

    Li Yuanhong

    2017-01-01

    Full Text Available Based on CAN bus, this paper studies the system control and management system of locomotive in mine, analyzes the working principle of locomotive system, gives the CAN bus scheme, hardware circuit design and CAN communication protocol, and implements long-distance, high-reliability communication function and remote monitoring function. Experiments show that the auxiliary system based on CAN bus control easier, operation more secure, as well as improving the control performance and service life of the electric locomotive.

  11. MODELLING AND ANALYSIS OF THE BUS PRIORITY IMPLEMENTATION

    Directory of Open Access Journals (Sweden)

    Lydia Hidayati

    2013-05-01

    Full Text Available Bus is expected to play an important role in providing a reliable travel in congested conditions. Therefore, it has been widely studied to minimize the travel time and the delay of the bus by proposing bus priority scheme to increase the attractiveness of public transport against private vehicles. Signal timing optimization combined with the bus priority scheme are discussed in this research as the alternatives to improve the intersection performances. Further, identifying the effects of these alternatives towards the general vehicles and public transport system performances were the aims of this research as well. As the result of this research, bus advance area with pre-signal method is able to reduce the travel time and delay of the bus for routes 2A and 3A by 11%, 23% and 14% , 0% respectively and able to reduce travel time, delay, and queue of vehicles by 25%, 26%, 7% consecutively. Thus bus advance area with pre-signal method is proposed as the best alternative due to its impact to reduce the bus travel times and delay without degrading the other vehicles performance. Keywords: bus priority, optimizing signal timing, Aimsun simulation, travel time-delay-queue.

  12. Model Passengers’ Travel Time for Conventional Bus Stop

    Directory of Open Access Journals (Sweden)

    Guangzhao Xin

    2014-01-01

    Full Text Available Limited number of berths can result in a subsequent bus stopping at the upstream of a bus stop when all berths are occupied. When this traffic phenomenon occurs, passengers waiting on the platform usually prefer walking to the stopped bus, which leads to additional walking time before boarding the bus. Therefore, passengers’ travel time consumed at a bus stop is divided into waiting time, additional walking time, and boarding time. This paper proposed a mathematical model for analyzing passengers’ travel time at conventional bus stop based on theory of stochastic service system. Field-measured and simulated data were designated to demonstrate the effectiveness of the proposed model. By analyzing the results, conclusion was conducted that short headway can reduce passengers’ waiting time at bus stop. Meanwhile, the theoretical analysis explained the inefficiency of bus stops with more than three berths from the perspective of passengers’ additional walking time. Additional walking time will increase in a large scale when the number of berths at a bus stop exceedsthe threshold of three.

  13. Depolarization-mediated regulation of alternative splicing

    Directory of Open Access Journals (Sweden)

    Alok eSharma

    2011-12-01

    Full Text Available Alternative splicing in eukaryotes plays an important role in regulating gene expression by selectively including alternative exons. A wealth of information has been accumulated that explains how alternative exons are selected in a developmental stage- or tissue-specific fashion. However, our knowledge of how cells respond to environmental changes to alter alternative splicing is very limited. For example, although a number of alternative exons have been shown to be regulated by calcium level alterations, the underlying mechanisms are not well understood. As calcium signaling in neurons plays a crucial role in essential neuronal functions such as learning and memory formation, it is important to understand how this process is regulated at every level in gene expression. The significance of the dynamic control of alternative splicing in response to changes of calcium levels has been largely unappreciated. In this communication, we will summarize the recent advances in calcium signaling-mediated alternative splicing that have provided some insights into the important regulatory mechanisms. In addition to describing the cis-acting RNA elements on the pre-mRNA molecules that respond to changes of intracellular calcium levels, we will summarize how splicing regulators change and affect alternative splicing in this process. We will also discuss a novel mode of calcium-mediated splicing regulation at the level of chromatin structure and transcription.

  14. Splicing pattern - ASTRA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us ASTRA Splicing pattern Data detail Data name Splicing pattern DOI 10.18908/lsdba.nbdc00371-0...04 Description of data contents The patterns of alternative splicing/transcriptional initiation Data file Fi...le name: astra_splicing_pattern.zip File URL: ftp://ftp.biosciencedbc.jp/archive/astra/LATEST/astra_splicing_pattern...ogodb/view/astra_splicing_pattern#en Data acquisition method For the five organisms (H. sapiens, M. musculus...apping data into bit arrays, detection of splicing patterns and distribution to t

  15. 77 FR 6178 - FY 2012 Discretionary Funding Opportunities: Bus and Bus Facilities Programs (State of Good...

    Science.gov (United States)

    2012-02-07

    ... transportation, environmental protection, and housing investments. Bus Livability will invest in projects that... characteristics of all communities by investing in healthy, safe and walkable neighborhoods--rural, urban or... guidelines. The project improves energy efficiency or reduces energy consumption/green house gas emissions...

  16. 76 FR 68819 - State of Good Repair Bus and Bus Facilities Discretionary Program Funds

    Science.gov (United States)

    2011-11-07

    ...--New York, Administrator, Region 7--Kansas One Bowling Green, Room 429, New City, MO, 901 Locust Street... transportation bus fleet, infrastructure, and equipment in a state of good repair. Project Implementation..., Region 6--Ft. Kendall Square, 55 Broadway, Suite Worth, 819 Taylor Street, Room 920, Cambridge, MA 02142...

  17. Extracting potential bus lines of Customized City Bus Service based on public transport big data

    Science.gov (United States)

    Ren, Yibin; Chen, Ge; Han, Yong; Zheng, Huangcheng

    2016-11-01

    Customized City Bus Service (CCBS) can reduce the traffic congestion and environmental pollution that caused by the increasing in private cars, effectively. This study aims to extract the potential bus lines and each line's passenger density of CCBS by mining the public transport big data. The datasets used in this study are mainly Smart Card Data (SCD) and bus GPS data of Qingdao, China, from October 11th and November 7th 2015. Firstly, we compute the temporal-origin-destination (TOD) of passengers by mining SCD and bus GPS data. Compared with the traditional OD, TOD not only has the spatial location, but also contains the trip's boarding time. Secondly, based on the traditional DBSCAN algorithm, we put forwards an algorithm, named TOD-DBSCAN, combined with the spatial-temporal features of TOD.TOD-DBSCAN is used to cluster the TOD trajectories in peak hours of all working days. Then, we define two variables P and N to describe the possibility and passenger destiny of a potential CCBS line. P is the probability of the CCBS line. And N represents the potential passenger destiny of the line. Lastly, we visualize the potential CCBS lines extracted by our procedure on the map and analyse relationship between potential CCBS lines and the urban spatial structure.

  18. Managing Student Behavior on the School Bus: A Key to Bus Safety

    Science.gov (United States)

    American Federation of Teachers (NJ), 2007

    2007-01-01

    School buses are the safest form of mass transit in America. Every day, school buses travel through congested city traffic and on isolated country roads in all types of weather, safely transporting millions of children to and from school and school-related activities. Federal and state standards for school bus construction and maintenance and…

  19. Simulation Model of Bus Rapid Transit

    Directory of Open Access Journals (Sweden)

    Gunawan Fergyanto E.

    2014-03-01

    Full Text Available Bus rapid transit system is modern solution for mass transportation system. The system, in comparison to the rail-based transportation system, is significantly cheaper and requires shorter development time, but lower performance. The BRT system performance strongly depends on variables related to station design and infrastructure. A numerical model offers an effective and efficient means to evaluate the system performance. This article offers a detailed numerical model on the basis of the discrete-event approach and demonstrates its application.

  20. Oracle Service Bus 11g Development Cookbook

    CERN Document Server

    Schmutz, Guido; van Zoggel, Jan

    2012-01-01

    This cookbook is full of immediately useable recipes showing you how to develop service and message-oriented (integration) applications on the Oracle Service Bus. In addition to its cookbook style, which ensures the solutions are presented in a clear step-by-step manner, the explanations go into great detail, which makes it good learning material for everyone who has experience in OSB and wants to improve. Most of the recipes are designed in such a way that each recipe is presented as a separate, standalone entity and reading of prior recipes is not required. The finished solution of each reci

  1. Redesign of Transjakarta Bus Driver's Cabin

    Science.gov (United States)

    Mardi Safitri, Dian; Azmi, Nora; Singh, Gurbinder; Astuti, Pudji

    2016-02-01

    Ergonomic risk at work stations with type Seated Work Control was one of the problems faced by Transjakarta bus driver. Currently “Trisakti” type bus, one type of bus that is used by Transjakarta in corridor 9, serving route Pinang Ranti - Pluit, gained many complaints from drivers. From the results of Nordic Body Map questionnaires given to 30 drivers, it was known that drivers feel pain in the neck, arms, hips, and buttocks. Allegedly this was due to the seat position and the button/panel bus has a considerable distance range (1 meter) to be achieved by drivers. In addition, preliminary results of the questionnaire using Workstation Checklist identified their complaints about uncomfortable cushion, driver's seat backrest, and the exact position of the AC is above the driver head. To reduce the risk level of ergonomics, then did research to design the cabin by using a generic approach to designing products. The risk analysis driver posture before the design was done by using Rapid Upper Limb Assessment (RULA), Rapid Entire Body Assessment (REBA), and Quick Exposure Checklist (QEC), while the calculation of the moment the body is done by using software Mannequin Pro V10.2. Furthermore, the design of generic products was done through the stages: need metric-matrix, house of quality, anthropometric data collection, classification tree concept, concept screening, scoring concept, design and manufacture of products in the form of two-dimensional. While the design after design risk analysis driver posture was done by using RULA, REBA, and calculation of moments body as well as the design visualized using software 3DMax. From the results of analysis before the draft design improvements cabin RULA obtained scores of 6, REBA 9, and the result amounted to 57.38% QEC and moment forces on the back is 247.3 LbF.inch and on the right hip is 72.9 LbF.in. While the results of the proposed improvements cabin design RULA obtained scores of 3, REBA 4, and the moment of force on

  2. Regular languages, regular grammars and automata in splicing systems

    Science.gov (United States)

    Mohamad Jan, Nurhidaya; Fong, Wan Heng; Sarmin, Nor Haniza

    2013-04-01

    Splicing system is known as a mathematical model that initiates the connection between the study of DNA molecules and formal language theory. In splicing systems, languages called splicing languages refer to the set of double-stranded DNA molecules that may arise from an initial set of DNA molecules in the presence of restriction enzymes and ligase. In this paper, some splicing languages resulted from their respective splicing systems are shown. Since all splicing languages are regular, languages which result from the splicing systems can be further investigated using grammars and automata in the field of formal language theory. The splicing language can be written in the form of regular languages generated by grammar. Besides that, splicing systems can be accepted by automata. In this research, two restriction enzymes are used in splicing systems namely BfuCI and NcoI.

  3. RNA-Binding Proteins: Splicing Factors and Disease

    Directory of Open Access Journals (Sweden)

    Alger M. Fredericks

    2015-05-01

    Full Text Available Pre-mRNA splicing is mediated by interactions of the Core Spliceosome and an array of accessory RNA binding proteins with cis-sequence elements. Splicing is a major regulatory component in higher eukaryotes. Disruptions in splicing are a major contributor to human disease. One in three hereditary disease alleles are believed to cause aberrant splicing. Hereditary disease alleles can alter splicing by disrupting a splicing element, creating a toxic RNA, or affecting splicing factors. One of the challenges of medical genetics is identifying causal variants from the thousands of possibilities discovered in a clinical sequencing experiment. Here we review the basic biochemistry of splicing, the mechanisms of splicing mutations, the methods for identifying splicing mutants, and the potential of therapeutic interventions.

  4. Heat shock activates splicing at latent alternative 5' splice sites in nematodes.

    Science.gov (United States)

    Nevo, Yuval; Sperling, Joseph; Sperling, Ruth

    2015-01-01

    Pre-mRNA splicing is essential for the regulation of gene expression in eukaryotes and is fundamental in development and cancer, and involves the selection of a consensus sequence that defines the 5' splice site (5'SS). Human introns harbor multiple sequences that conform to the 5'SS consensus, which are not used under normal growth conditions. Under heat shock conditions, splicing at such intronic latent 5'SSs occurred in thousands of human transcripts, resulting in pre-maturely terminated aberrant proteins. Here we performed a survey of the C. elegans genome, showing that worm's introns contain latent 5'SSs, whose use for splicing would have resulted in pre-maturely terminated mRNAs. Splicing at these latent 5'SSs could not be detected under normal growth conditions, while heat shock activated latent splicing in a number of tested C. elegans transcripts. Two scenarios could account for the lack of latent splicing under normal growth conditions (i) Splicing at latent 5'SSs do occur, but the nonsense mRNAs thus formed are rapidly and efficiently degraded (e.g. by NMD); and (ii) Splicing events at intronic latent 5'SSs are suppressed. Here we support the second scenario, because, nematode smg mutants that are devoid of NMD-essential factors, did not show latent splicing under normal growth conditions. Hence, these experiments together with our previous experiments in mammalian cells, indicate the existence of a nuclear quality control mechanism, termed Suppression Of Splicing (SOS), which discriminates between latent and authentic 5'SSs in an open reading frame dependent manner, and allows splicing only at the latter. Our results show that SOS is an evolutionary conserved mechanism, probably shared by most eukaryotes.

  5. Effect of stoppage time on motion of a bus through a sequence of signals

    Science.gov (United States)

    Nagatani, Takashi

    2017-01-01

    We study the dynamic motion of a bus moving through the series of traffic signals where the bus stops at bus stops during a time. The dynamic state of the bus depends highly on both stoppage time at the bus stop and cycle time of the signal. It is found that the bus motion has two kinds of dynamic states: the one is the normal state and the other is the offset state. In the normal state, the bus stops normally at both bus stops and signals. In the offset state, the bus passes timely through the signal sometimes and the stoppage time at the bus stop is offset against the stopping time at the signal. If the bus speed has the same value as the car speed, the travel time of the bus is consistent with that of the car in the offset state. The region map (phase diagram) is shown for two kinds of dynamic states: the normal and offset states.

  6. A Conceptual Approach for Optimising Bus Stop Spacing

    Science.gov (United States)

    Johar, Amita; Jain, S. S.; Garg, P. k.

    2017-06-01

    An efficient public transportation system is essential of any country. The growth, development and shape of the urban areas are mainly due to availability of good transportation (Shah et al. in Inst Town Plan India J 5(3):50-59, 1). In developing countries, like India, travel by local bus in a city is very common. The accidents, congestion, pollution and appropriate location of bus stops are the major problems arising in metropolitan cities. Among all the metropolitan cities in India, Delhi has highest percentage of growth of population and vehicles. Therefore, it is important to adopt efficient and effective ways to improve mobility in different metropolitan cities in order to overcome the problem and to reduce the number of private vehicles on the road. The primary objective of this paper is to present a methodology for developing a model for optimum bus stop spacing (OBSS). It describes the evaluation of existing urban bus route, data collection, development of model for optimizing urban bus route and application of model. In this work, the bus passenger generalized cost method is used to optimize the spacing between bus stops. For the development of model, a computer program is required to be written. The applicability of the model has been evaluated by taking the data of urban bus route of Delhi Transport Corporation (DTC) in Excel sheet in first phase. Later on, it is proposed to develop a programming in C++ language. The developed model is expected to be useful to transport planner for rational design of the spacing of bus stops to save travel time and to generalize operating cost. After analysis it is found that spacing between the bus stop comes out to be between 250 and 500 m. The Proposed Spacing of bus stops is done considering the points that they don't come nearer to metro/rail station, entry or exit of flyover and near traffic signal.

  7. SpliceAid-F: a database of human splicing factors and their RNA-binding sites.

    Science.gov (United States)

    Giulietti, Matteo; Piva, Francesco; D'Antonio, Mattia; D'Onorio De Meo, Paolo; Paoletti, Daniele; Castrignanò, Tiziana; D'Erchia, Anna Maria; Picardi, Ernesto; Zambelli, Federico; Principato, Giovanni; Pavesi, Giulio; Pesole, Graziano

    2013-01-01

    A comprehensive knowledge of all the factors involved in splicing, both proteins and RNAs, and of their interaction network is crucial for reaching a better understanding of this process and its functions. A large part of relevant information is buried in the literature or collected in various different databases. By hand-curated screenings of literature and databases, we retrieved experimentally validated data on 71 human RNA-binding splicing regulatory proteins and organized them into a database called 'SpliceAid-F' (http://www.caspur.it/SpliceAidF/). For each splicing factor (SF), the database reports its functional domains, its protein and chemical interactors and its expression data. Furthermore, we collected experimentally validated RNA-SF interactions, including relevant information on the RNA-binding sites, such as the genes where these sites lie, their genomic coordinates, the splicing effects, the experimental procedures used, as well as the corresponding bibliographic references. We also collected information from experiments showing no RNA-SF binding, at least in the assayed conditions. In total, SpliceAid-F contains 4227 interactions, 2590 RNA-binding sites and 1141 'no-binding' sites, including information on cellular contexts and conditions where binding was tested. The data collected in SpliceAid-F can provide significant information to explain an observed splicing pattern as well as the effect of mutations in functional regulatory elements.

  8. Protein splicing and its evolution in eukaryotes

    Directory of Open Access Journals (Sweden)

    Starokadomskyy P. L.

    2010-02-01

    Full Text Available Inteins, or protein introns, are parts of protein sequences that are post-translationally excised, their flanking regions (exteins being spliced together. This process was called protein splicing. Originally inteins were found in prokaryotic or unicellular eukaryotic organisms. But the general principles of post-translation protein rearrangement are evolving yielding different post-translation modification of proteins in multicellular organisms. For clarity, these non-intein mediated events call either protein rearrangements or protein editing. The most intriguing example of protein editing is proteasome-mediated splicing of antigens in vertebrates that may play important role in antigen presentation. Other examples of protein rearrangements are maturation of Hg-proteins (critical receptors in embryogenesis as well as maturation of several metabolic enzymes. Despite a lack of experimental data we try to analyze some intriguing examples of protein splicing evolution.

  9. Djungle power. A more remote AC bus

    Energy Technology Data Exchange (ETDEWEB)

    Boehnke, Heinz W. [Technosol, Jork (Germany)

    2010-07-01

    The Dzanga-Sangha Reserve in the Central African Republic is probably one of the world's most remote protected rainforest areas, much to the benefit of the rare species of lowland gorilla, forest elephant and local pygmy population Ba'Aka. The park is co-managed by the ''WWF-World Wide Fund for Nature'' with sponsoring from the German Regenwald-Stiftung. In 2008 wwf decided to switch from the old, failing generator supply to a solar hybrid mini-grid, along with stringent measures of energy conservation and management. After a careful energy audit Technosol designed an AC-bus system with a 22 kWp PV generator which should overcome the dependency on the old generators and their fuel demands. For 15 buildings, an entirely new distribution network was installed, state of the art energy saving devices introduced and an energy metering system deviced. The installation is operating since August 2009. In such remote locations, the reliability of the AC-bus over other generator-based solutions will be demonstrated and with the support of international organisations like the wwf the practical application of solar hybrid supply becomes a landmark of sustainable energy concepts. (orig.)

  10. Implementation of viscoelastic Hopkinson bars

    Directory of Open Access Journals (Sweden)

    Govender R.

    2012-08-01

    Full Text Available Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic nature of the bar material. Implementing polymeric Hopkinson bars requires characterization of the viscoelastic properties of the material used. In this paper, 30 mm diameter Polymethyl Methacrylate bars are used as Hopkinson pressure bars. This testing technique is applied to polymeric foam called Divinycell H80 and H200. Although there is a large body of of literature containing compressive data, this rarely deals with strain rates above 250s−1 which becomes increasingly important when looking at the design of composite structures where energy absorption during impact events is high on the list of priorities. Testing of polymeric foams at high strain rates allows for the development of better constitutive models.

  11. Implementation of viscoelastic Hopkinson bars

    Science.gov (United States)

    Curry, R.; Cloete, T.; Govender, R.

    2012-08-01

    Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic nature of the bar material. Implementing polymeric Hopkinson bars requires characterization of the viscoelastic properties of the material used. In this paper, 30 mm diameter Polymethyl Methacrylate bars are used as Hopkinson pressure bars. This testing technique is applied to polymeric foam called Divinycell H80 and H200. Although there is a large body of of literature containing compressive data, this rarely deals with strain rates above 250s-1 which becomes increasingly important when looking at the design of composite structures where energy absorption during impact events is high on the list of priorities. Testing of polymeric foams at high strain rates allows for the development of better constitutive models.

  12. Towards formal verification of ToolBus scripts

    NARCIS (Netherlands)

    Fokkink, W.; Klint, P.; Lisser, B.; Usenko, Y.S.

    2008-01-01

    ToolBus allows one to connect tools via a software bus. Programming is done using the scripting language Tscript, which is based on the process algebra ACP. Tscript was originally designed to enable formal verification, but this option has so far not been explored in any detail. We present a method

  13. Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus: Preliminary Evaluation Results

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K.; Eudy, L.

    2008-10-01

    This report provides preliminary results from a National Renewable Energy Laboratory evaluation of a protoptye fuel cell transit bus operating at Connecticut Transit in Hartford. Included are descriptions of the planned fuel cell bus demonstration and equipment; early results and agency experience are also provided.

  14. Solar Telescope Control with the CAN-Bus

    Science.gov (United States)

    Pettauer, T. V.

    The real time behavior, error handling and its simplicity makes CAN a preferable bus protocol to interconnect the various modules of a tower telescope. A schematic description of the control bus for the Kanzelhöhe Vakuum Telescope is given.

  15. Application of CAN-bus Network in Intelligent parking

    Science.gov (United States)

    Jianjun, WU; Juan, Hu

    Convenient life makes intelligent parking every- where. This paper describes how to connect the various separate modern terminal of intelligent parking with CAN-bus network, and the competitive advantage of the system by using the CAN-bus network. From a technical point of view, this paper describes the development trend of intelligent parking in next few years.

  16. Connecticut nutmeg fuel cell bus project : first analysis report.

    Science.gov (United States)

    2012-07-01

    This report summarizes the experience and early results from a fuel cell bus demonstration funded by the Federal Transit Administra-tion (FTA) under the National Fuel Cell Bus Program (NFCBP). A team led by the Northeast Advanced Vehicle Consortium a...

  17. Bus-based best-practice and urban transport emissions

    Science.gov (United States)

    Enoch, Marcus Paul

    In Britain over recent years, transport has risen to the very top of the political agenda, partly because it is the fastest growing source of urban emissions. One politically attractive solution to this rapidly worsening problem, is 'improving public transport'. This thesis examines how the bus-the most widespread form of public transport-could be improved, and at how much these improvements would contribute to solving the emissions problem. The project was studied in two inter-related parts. The first part aimed to determine the factors necessary in forming the 'best possible' bus-based transport system by identifying and analysing a number of 'best-practice' bus systems in Europe and North America, using a case study approach. The second part then sought to quantify the effect on travel patterns and emissions that implementing this best possible bus-based transport system would have. This was done by modelling a series of possible scenarios with the START strategic transport model, and a modified version of the JET emissions inventory model. The City of Leicester was used as a modelling base. The study found that bus systems contributed most to reducing urban emissions where the needs of the bus passenger were made paramount, and where bus operators worked together with local government, local businesses etc. Even where these factors existed however, the effect of bus-based transport policies on levels of pollutants was found to be significant only when applied together with strong car restraint measures.

  18. Bus Lifecycle Cost Model for Federal Land Management Agencies.

    Science.gov (United States)

    2011-09-30

    The Bus Lifecycle Cost Model is a spreadsheet-based planning tool that estimates capital, operating, and maintenance costs for various bus types over the full lifecycle of the vehicle. The model is based on a number of operating characteristics, incl...

  19. 32 CFR 935.138 - Motor bus operation.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Motor bus operation. 935.138 Section 935.138 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND CODE Motor Vehicle Code § 935.138 Motor bus operation. Each person operating a motor...

  20. Network Analysis of Urban Traffic with Big Bus Data

    CERN Document Server

    Zhao, Kai

    2016-01-01

    Urban traffic analysis is crucial for traffic forecasting systems, urban planning and, more recently, various mobile and network applications. In this paper, we analyse urban traffic with network and statistical methods. Our analysis is based on one big bus dataset containing 45 million bus arrival samples in Helsinki. We mainly address following questions: 1. How can we identify the areas that cause most of the traffic in the city? 2. Why there is a urban traffic? Is bus traffic a key cause of the urban traffic? 3. How can we improve the urban traffic systems? To answer these questions, first, the betweenness is used to identify the most import areas that cause most traffics. Second, we find that bus traffic is not an important cause of urban traffic using statistical methods. We differentiate the urban traffic and the bus traffic in a city. We use bus delay as an identification of the urban traffic, and the number of bus as an identification of the bus traffic. Third, we give our solutions on how to improve...

  1. Thermopriming Triggers Splicing Memory in Arabidopsis

    KAUST Repository

    Ling, Yu

    2018-02-20

    Abiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred to as priming, can allow plants to survive subsequent and otherwise lethal conditions; the priming effect persists even after a prolonged stress-free period. However, the molecular mechanisms underlying priming are not fully understood. Here, we investigated the molecular basis of heat shock memory and the role of priming in Arabidopsisthaliana. Comprehensive analysis of transcriptome-wide changes in gene expression and alternative splicing in primed and non-primed plants revealed that alternative splicing functions as a novel component of heat shock memory. We show that priming of plants with a non-lethal heat stress results in de-repression of splicing after a second exposure to heat stress. By contrast, non-primed plants showed significant repression of splicing. These observations link ‘splicing memory’ to the ability of plants to survive subsequent and otherwise lethal heat stress. This newly discovered priming-induced splicing memory may represent a general feature of heat stress responses in plants and other organisms as many of the key components of heat shock responses are conserved among eukaryotes. Furthermore, this finding could facilitate the development of novel approaches to improve plant survival under extreme heat stress.

  2. A series of small scientific satellite with flexible standard bus

    Science.gov (United States)

    Saito, Hirobumi; Sawai, Syujiro; Sakai, Shin-ichiro; Fukuda, Seisuke; Kitade, Kenji

    2009-11-01

    Japan Aerospace Exploration Agency has a plan to develop the small satellite standard bus for various scientific missions and disaster monitoring missions. The satellite bus is a class of 250-400 kg mass with three-axis control capability of 0.02∘ accuracy. The science missions include X-ray astronomy missions, planetary telescope missions, and magnetosphere atmosphere missions. In order to adapt the wide range of mission requirements, the satellite bus has to be provided with flexibility. The concepts of modularization, reusability, and product line are applied to the standard bus system. This paper describes the characteristics of the small satellite standard bus which will be firstly launched in 2011.

  3. Research on the Coordinated Design of Bus and Taxi Station

    Directory of Open Access Journals (Sweden)

    Jiangfeng Xi

    2015-01-01

    Full Text Available Rises in the number of transit buses, bus routes, and overall traffic volume in China’s cities, coupled with interference from other transport modes, such as taxis loading and unloading passengers nearby, have led to increasing traffic delays at bus stops, which is considered one of the factors degrading service levels and traffic operations on urban roadways. This paper studies traffic characteristics at bus stops, investigates variations in delay from different types or designs of bus stops, and analyzes the impact of it on traffic capacity, the purpose of which is to propose a solution to predicting the feasibility of an integrated design of bus stops and taxi stands with the help of mathematical models and based on the objectives of optimal traffic operations and passenger transfer.

  4. Genetic Algorithm for Multiple Bus Line Coordination on Urban Arterial

    Directory of Open Access Journals (Sweden)

    Zhen Yang

    2015-01-01

    Full Text Available Bus travel time on road section is defined and analyzed with the effect of multiple bus lines. An analytical model is formulated to calculate the total red time a bus encounters when travelling along the arterial. Genetic algorithm is used to optimize the offset scheme of traffic signals to minimize the total red time that all bus lines encounter in two directions of the arterial. The model and algorithm are applied to the major part of Zhongshan North Street in the city of Nanjing. The results show that the methods in this paper can reduce total red time of all the bus lines by 31.9% on the object arterial and thus improve the traffic efficiency of the whole arterial and promote public transport priority.

  5. Double-Barred Galaxies: I. A Catalog of Barred Galaxies with Stellar Secondary Bars and Inner Disks

    OpenAIRE

    Erwin, Peter

    2003-01-01

    I present a catalog of 67 barred galaxies which contain distinct, elliptical stellar structures inside their bars. Fifty of these are double-barred galaxies: a small-scale, "inner" or "secondary" bar is embedded within a large-scale, "outer" or "primary" bar. I provide homogenized measurements of the sizes, ellipticities, and orientations of both inner and outer bars, along with with global parameters for the galaxies. The other 17 are classified as "inner-disk" galaxies, where a large-scale ...

  6. Bar Coliseo, en Sevilla

    Directory of Open Access Journals (Sweden)

    de la Peña Neila, Antonio

    1963-10-01

    Full Text Available This bar is situated inside the «Coliseo» building, which houses a cinema, as well as a number of commercial establishments. In order not to break the unity of the total project, no attempt has been made to alter the exterior aspect of the bar. No attempt was made, either, to make it into an intimate, club type of bar, now so much in fashion. Rather has it been given a diaphanous style, seeking the best possible use of the floor space. The windows of the building are elongated, and there is an intermediate floor level, whose detailed structure is metallic. A cleverly designed staircase, of folded sheet metal connects the ground floor, the intermediate floor level and the restaurant. Materials were carefully chosen in accordance with their function. The colour scheme has a sustained unity throughout the building, and care has been taken to avoid surprising or vivid chromatic patterns. Ceramic enamels by the painter Santiago del Campo provide a feature of decoration on the ground floor, and also serve to cover up the return air ducts. On the top floor, the restaurant is fitted with coloured tile facings, the work of the Seville painters Maria Josefa Sánchez, María Dolores Sánchez and Emilio García Ortiz. The bottom joints of the timber beams, in conjunction with the tile patterns, is reminiscent of the traditional Sevillian habit of placing ceramic units between the timber framework of buildings. The initial problem of the architect was to combine the optimum functional efficiency and aesthetic quality of the project, and the final solution is undoubtedly successful.El establecimiento está situado dentro del edificio «Coliseo», complejo formado por una sala de cine, y con la parte lateral destinada a locales comerciales. Formando un conjunto único no se pensó nunca en transformar los revestimientos y molduras de fachada. Tampoco presidió la idea de conseguir un establecimiento íntimo «tipo Club», tan en boga actualmente, sino un

  7. Recommendations for yield-to-bus traffic control devices and bus pullout bays design characteristics : [research summary].

    Science.gov (United States)

    2013-04-01

    Bus pullout bays are being installed increasingly : on Floridas roads as a safety factor and so that : stopped buses do not impede traffic. In Florida, : state law requires car drivers to yield to buses : when a bus needs to reenter the roadway af...

  8. Work fatigue in urban bus drivers

    Directory of Open Access Journals (Sweden)

    Teresa Makowiec-Dąbrowska

    2015-10-01

    Full Text Available Background: Bus drivers are a special group of professional drivers who are at a very high risk of fatigue. The aim of the study was to examine whether the driver’s subjective assessment of fatigue allows for the determination of its level and identification of its causes. Material and Methods: The study group comprised 45 randomly selected bus drivers (mean age – 43.7±7.9 years, period of employment as drivers – 14.7±8.6 years. Examinations were performed in all subjects four times – before and after work on the “easy” route (outside the city center, small traffic intensity and before and after work on the “difficult” route (city center, heavy traffic. The fatigue test questionnaire, based on the list of symptoms of fatigue prepared by the Japan Research Committee of Fatigue, was used in the study. Results: The rating of fatigue after the work was significantly higher than that before the work. The profile of fatigue after work was not influenced by the type of route, but the assessment of most symptoms of fatigue reached a higher level after the “difficult” routes and the differences were statistically significant for 7 symptoms. Only the ratings of leg fatigue, feeling of heaviness, and the necessity to squint eyes and gaze with effort reached the higher levels after driving the “easy” routes. It has been found that the level of fatigue was significantly correlated with the job characteristics (driving time, the length of the route, number of stops, etc. and with the abundance of food ingested and type of beverage (coffee vs. others drunk prior to driving. Conclusions: The questionnaire used in our study to assess the subjective feeling of fatigue has proved to be a sensitive and useful tool for indicating the level and causes of fatigue. The relationship between the symptoms of fatigue and the characteristics of job and lifestyle shows that actions must be taken by both the employers and employees to prevent fatigue

  9. Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Daniel Nilsson

    2010-08-01

    Full Text Available Trans-splicing of leader sequences onto the 5'ends of mRNAs is a widespread phenomenon in protozoa, nematodes and some chordates. Using parallel sequencing we have developed a method to simultaneously map 5'splice sites and analyze the corresponding gene expression profile, that we term spliced leader trapping (SLT. The method can be applied to any organism with a sequenced genome and trans-splicing of a conserved leader sequence. We analyzed the expression profiles and splicing patterns of bloodstream and insect forms of the parasite Trypanosoma brucei. We detected the 5' splice sites of 85% of the annotated protein-coding genes and, contrary to previous reports, found up to 40% of transcripts to be differentially expressed. Furthermore, we discovered more than 2500 alternative splicing events, many of which appear to be stage-regulated. Based on our findings we hypothesize that alternatively spliced transcripts present a new means of regulating gene expression and could potentially contribute to protein diversity in the parasite. The entire dataset can be accessed online at TriTrypDB or through: http://splicer.unibe.ch/.

  10. How to Find a Comfortable Bus Route - Towards Personalized Information Recommendation Services

    Directory of Open Access Journals (Sweden)

    Danhuai Guo

    2015-05-01

    Full Text Available With the generation of massive data from bus IC cards, how to effectively and efficiently recommend comfortable bus routes to bus passengers is a challenging and complex task. In this paper, waiting time, crowded time, and driving time between different bus stations on different bus routes at different times of the day are calculated from bus IC cards data history. Then, a multi-objective program with various constraints is suggested to recommend comfortable bus routes for bus passengers, and a genetic algorithm is developed to search for expected solutions. The proposed method is implemented using bus IC cards data from Chongqing, China and will be a promising tool for bus passengers when choosing comfortable bus routes.

  11. A Self-Adjusting Method to Resist Bus Bunching Based on Boarding Limits

    Directory of Open Access Journals (Sweden)

    Shuzhi Zhao

    2016-01-01

    Full Text Available Bus bunching is one of the most serious problems of urban bus systems. Bus bunching increases waiting and travel time of passengers. Many bus systems use schedules to reach equal headways. Compared to the idea of schedules and the target headway introduced later, we propose a new method to improve the efficiency of a bus system and avoid bus bunching by boarding limits. Our solution can be effectively implemented when buses cannot travel as planned because of bad road conditions and dynamic demands at bus stops. Besides, using our method, bus headways reach the state with equal headways dynamically and spontaneously without drivers’ explicit intervention. Moreover, the method can improve the level of the bus service and reduce total travel time of passengers. We verify our method using an ideal bus route and a real bus route, both showing the success of the proposed method.

  12. Bus industry market study. Report -- Task 3.2: Fuel cell/battery powered bus system

    Energy Technology Data Exchange (ETDEWEB)

    Zalbowitz, M.

    1992-06-02

    In support of the commercialization of fuel cells for transportation, Georgetown University, as a part of the DOE/DOT Fuel Cell Transit Bus Program, conducted a market study to determine the inventory of passenger buses in service as of December, 1991, the number of buses delivered in 1991 and an estimate of the number of buses to be delivered in 1992. Short term and long term market projections of deliveries were also made. Data was collected according to type of bus and the field was divided into the following categories which are defined in the report: transit buses, school buses, commercial non-transit buses, and intercity buses. The findings of this study presented with various tables of data collected from identified sources as well as narrative analysis based upon interviews conducted during the survey.

  13. Differential splicing using whole-transcript microarrays

    Directory of Open Access Journals (Sweden)

    Robinson Mark D

    2009-05-01

    Full Text Available Abstract Background The latest generation of Affymetrix microarrays are designed to interrogate expression over the entire length of every locus, thus giving the opportunity to study alternative splicing genome-wide. The Exon 1.0 ST (sense target platform, with versions for Human, Mouse and Rat, is designed primarily to probe every known or predicted exon. The smaller Gene 1.0 ST array is designed as an expression microarray but still interrogates expression with probes along the full length of each well-characterized transcript. We explore the possibility of using the Gene 1.0 ST platform to identify differential splicing events. Results We propose a strategy to score differential splicing by using the auxiliary information from fitting the statistical model, RMA (robust multichip analysis. RMA partitions the probe-level data into probe effects and expression levels, operating robustly so that if a small number of probes behave differently than the rest, they are downweighted in the fitting step. We argue that adjacent poorly fitting probes for a given sample can be evidence of differential splicing and have designed a statistic to search for this behaviour. Using a public tissue panel dataset, we show many examples of tissue-specific alternative splicing. Furthermore, we show that evidence for putative alternative splicing has a strong correspondence between the Gene 1.0 ST and Exon 1.0 ST platforms. Conclusion We propose a new approach, FIRMAGene, to search for differentially spliced genes using the Gene 1.0 ST platform. Such an analysis complements the search for differential expression. We validate the method by illustrating several known examples and we note some of the challenges in interpreting the probe-level data. Software implementing our methods is freely available as an R package.

  14. Organizing a bus depot – selected aspects

    Directory of Open Access Journals (Sweden)

    Jerzy PAWLICKI

    2012-01-01

    Full Text Available Urban transportation problems are particularly noticeable within cities of over 100 thousand inhabitants. In these cities buses are a primary means of transport, but lack of low-floor and poor interior design does not encourage passengers to use them. However, the qualities that give the most negative feedback on the public transpiration are the technical condition of buses, excessive noise and exhaust emission. Technical backsides of the bus depots that constantly maintain the technical readiness of the required number of vehicles play an important role to ensure high quality standards. New European Union legislation is very helpful in such. New laws and regulations deal with the safety issues and environmental protection matters, to which the urban transportation depots must be strictly adjusted. This article refers to these documents and presents depot technical stations, which equipment needs to be upgraded in the first place.

  15. Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture.

    Science.gov (United States)

    Kataoka, Naoyuki

    2016-01-01

    In eukaryotes, pre-mRNA splicing is an essential step for gene expression. Splicing reactions have been well investigated by using in vitro splicing reactions with extracts prepared from cultured cells. Here, we describe protocols for the preparation of splicing-competent extracts from cells expressing a tagged spliceosomal protein. The whole-cell extracts are able to splice exogenously added pre-mRNA and the RNA-protein complex formed in the in vitro splicing reaction can be purified by immunoprecipitation using antibodies against the peptide tag on the splicing protein. The method described here to prepare splicing-active extracts from whole cells is particularly useful when studying pre-mRNA splicing in various cell types, and the expression of a tagged spliceosomal protein allows one to purify and analyze the RNA-protein complexes by simple immunoprecipitation.

  16. Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

    Directory of Open Access Journals (Sweden)

    Elela Sherif

    2006-01-01

    Full Text Available Abstract Background We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins. In the present study, we have tested the inhibitory activity of RNA oligonucleotides carrying different tail structures. Results We show that an oligonucleotide with a 5' tail containing the human β-globin branch site sequence inhibits the use of the 5' splice site of Bcl-xL, albeit less efficiently than a tail containing binding sites for the hnRNP A1/A2 proteins. A branch site-containing tail positioned at the 3' end of the oligonucleotide also elicited splicing inhibition but not as efficiently as a 5' tail. The interfering activity of a 3' tail was improved by adding a 5' splice site sequence next to the branch site sequence. A 3' tail carrying a Y-shaped branch structure promoted similar splicing interference. The inclusion of branch site or 5' splice site sequences in the Y-shaped 3' tail further improved splicing inhibition. Conclusion Our in vitro results indicate that a variety of tail architectures can be used to elicit splicing interference at low nanomolar concentrations, thereby broadening the scope and the potential impact of this antisense technology.

  17. Research of WLAN CAN Bus Data Test System

    Science.gov (United States)

    Wu, Weibin; Zhu, Yuqing; Hong, Tiansheng; Ye, Cheng; Ye, Zhijie; He, Guangbin; Li, Haobiao

    Both controller area network (CAN) and wireless local area network (WLAN) bus technologies are developing rapidly nowadays. But the study on WLAN technology with CAN bus data is relative to be less researched. So a WLAN CAN bus data test system was present in this paper. The system hardware consists of two control modules, each of which consistently allows link test to the CAN bus network. Each hardware of the module is composed by a microcontroller STC12C5410AD, a CAN bus control unit MCP2515, a CAN bus transceiver MCP2551 and a RF wireless module RF903. During the test process, the CAN bus analyzer, Kvaser USBCAN II, receives and dispatches the CAN messages. Test results showed that the system's error score was approximately zero when messages are transmitted and the system can message transmission from one side of CAN panel point to the other side. The communication of the system is suitable for the CAN treaty standard and it can allow wireless message transmission to other suitable networks meeting the CAN treaty standard as well. Finally the system has been applied to the project for hill citrus leaf area index (LAI) acquisition.

  18. The BaBar Mini

    Energy Technology Data Exchange (ETDEWEB)

    Brown, David N.

    2003-07-11

    BaBar has recently deployed a new event data format referred to as the Mini. The mini uses efficient packing and aggressive noise suppression to represent the average reconstructed BaBar event in under 7 KBytes. The Mini packs detector information into simple transient data objects, which are then aggregated into roughly 10 composite persistent objects per event. The Mini currently uses Objectivity persistence, and it is being ported to use Root persistence. The Mini contains enough information to support detailed detector studies, while remaining small and fast enough to be used directly in physics analysis. Mini output is customizable, allowing users to both truncate unnecessary content or add content, depending on their needs. The Mini has now replaced three older formats as the primary output of BaBar event reconstruction. A reduced form of the Mini will soon replace the physics analysis format as well, giving BaBar a single, flexible event data format covering all its needs.

  19. Using microcomputers for lighting appliance control using a DALI bus

    Directory of Open Access Journals (Sweden)

    Sysala Tomas

    2016-01-01

    Full Text Available Article concerns the DALI bus theoretical and practical aspects. The DALI bus is a modern technology developed especially for illumination systems control. Authors are outlining communication basic principles related to the physical layer and going up to more complex data transactions among particular devices. The article main topic is aimed at two device models development based on microcontroller and exploiting that technology. The first of those two devices is a DALI slave unit enabling the light source or multi switch with light indication components control via DALI bus. The second device measures the ambient illumination intensity and it also detects errors in the load.

  20. Design and Research on Automotive Controller Area Network Bus Analyzer

    Directory of Open Access Journals (Sweden)

    Hongwei CUI

    2014-03-01

    Full Text Available The detection method of automotive controller area network bus is researched in this paper. Failure identifying of CAN bus under different working conditions has been realized. In order to realizing intelligent failure diagnosis, data fusion means has been put forward in this paper. The composition of analysis and detection system is introduced. By analyzing and processing the data of CAN bus and sensors, work condition of automotive is achieved. Multi-pattern data fusion model and algorithm for failure diagnosis are researched. The analyzer and detection system designed in this paper can be applied to automotive fault analysis, troubleshooting and maintenance.

  1. Solving of school bus routing problem by ant colony optimization

    OpenAIRE

    Arias-Rojas, Juan S.; Jiménez-Gordillo, J. F. (José Fernando); Montoya-Torres, J. R. (Jairo Rafael)

    2011-01-01

    The school bus routing problem (SBRP) seeks to plan an efficient schedule of a fleet of school buses that must pick up students from various bus stops and deliver them by satisfying various constraints: maximum capacity of the bus, maximum riding time of students, time window to arrive to school. In this paper, we consider a case study of SBRP for a school in Bogotá, Colombia. The problem is solved using ant colony optimization (ACO). Computational experiments are performed using real data. R...

  2. A new MV bus transfer scheme for nuclear power plants

    Directory of Open Access Journals (Sweden)

    Chang Choong-Koo

    2015-01-01

    Full Text Available Fast bus transfer method is the most popular and residual voltage transfer method that is used as a backup in medium voltage buses in general. The use of the advanced technology like open circuit voltage prediction and digital signal processing algorithms can improve the reliability of fast transfer scheme. However, according to the survey results of the recent operation records in nuclear power plants, there were many instances where the fast transfer scheme has failed. To assure bus transfer in any conditions and circumstances, uninterruptible bus transfer scheme utilizing the state of the art medium voltage UPS is discussed and elaborated.

  3. Swisstrolley 3 - An articulated trolley bus; SWISSTROLLEY 3. Gelenktrolleybus

    Energy Technology Data Exchange (ETDEWEB)

    Gisler, H.-J.

    2006-07-01

    This illustrated final report for the Swiss Federal Office of Energy (SFOE) takes a look at the Swisstrolley 3 low-floor, articulated trolley bus developed in Switzerland by the Hess AG company. Various features of the variants of the trolley bus, including single vehicle, single and double-articulated buses and bus trailers, are discussed. The reductions achieved in energy consumption, weight and noise are discussed. Typical values for power consumption are presented in graphical form. Economic viability and passenger-friendliness are examined, as are possibilities for further improvement.

  4. Research on Optimization for Motion Control Bus Based on Ethernet

    Directory of Open Access Journals (Sweden)

    Kai Sun

    2013-01-01

    Full Text Available Field bus system has been successfully introduced into industrial automation. Nowadays, most of the motion control bus is based on the Ethernet physical layer, and all of the new standards are based on the Ethernet physical layer. This paper introduces a new optimized technology based on motion control bus of the Ethernet physical layer, which includes the prediction mechanism in the arrival of the frames, the retransmission mechanism in advance, and a new mechanism of independent verification of data segment. Tests show that using the mechanisms in this paper can enhance efficiency and reliability in communication process.

  5. Common Airborne Instrumentation System: CAIS Bus Interface Standard, A00.00-C001. Revision A

    National Research Council Canada - National Science Library

    Jones, Sidney

    1997-01-01

    .... This interface control document (ICD) was written to provide a single document that designers of CAIS bus controllers and data acquisition units could reference to ensure interoperability on the CAIS bus. This ICD establishes the requirements for digital command/response, time division multiplexing techniques for a single CAIS bus. It encompasses the physical, electrical, and protocol aspects of the CAIS bus.

  6. Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF.

    Science.gov (United States)

    Chen, Zhiqi; Ma, Xuezhong; Zhang, Jianhua; Hu, Jim; Gorczynski, Reginald M

    2010-10-01

    CD200, a type I membrane glycoprotein, plays an important role in prevention of inflammatory disorders, graft rejection, autoimmune diseases and spontaneous fetal loss. It also regulates tumor immunity. A truncated CD200 (CD200(tr)) resulting from alternative splicing has been identified and characterized as a functional antagonist to full-length CD200. Thus, it is important to explore the mechanism(s) controlling alternative splicing of CD200. In this study, we identified an exonic splicing enhancer (ESE) located in exon 2, which is a putative binding site for a splicing regulatory protein SF2/ASF. Deletion or mutation of the ESE site decreased expression of the full-length CD200. Direct binding of SF2/ASF to the ESE site was confirmed by RNA electrophoretic mobility shift assay (EMSA). Knockdown of expression of SF2/ASF resulted in the same splicing pattern as seen after deletion or mutation of the ESE, whereas overexpression of SF2/ASF increased expression of the full-length CD200. In vivo studies showed that viral infection reversed the alternative splicing pattern of CD200 with increased expression of SF2/ASF and the full-length CD200. Taken together, our data suggest for the first time that SF2/ASF regulates the function of CD200 by controlling CD200 alternative splicing, through direct binding to an ESE located in exon 2 of CD200.

  7. Splicing variants of porcine synphilin-1

    DEFF Research Database (Denmark)

    Larsen, Knud Erik; Madsen, Lone Bruhn; Farajzadeh, Leila

    2015-01-01

    %) and to mouse (84%) synphilin-1. Three shorter transcript variants of the synphilin-1 gene were identified, all lacking one or more exons. SNCAIP transcripts were detected in most examined organs and tissues and the highest expression was found in brain tissues and lung. Conserved splicing variants and a novel......RNA was investigated by RNAseq. The presented work reports the molecular cloning and characterization of the porcine (Sus scrofa) synphilin-1 cDNA (SNCAIP) and three splice variants hereof. The porcine SNCAIP cDNA codes for a protein (synphilin-1) of 919 amino acids which shows a high similarity to human (90...... splice form of synhilin-1 were found in this study. All synphilin-1 isoforms encoded by the identified transcript variants lack functional domains important for protein degradation....

  8. Capillary Electrophoresis Analysis of Conventional Splicing Assays

    DEFF Research Database (Denmark)

    de Garibay, Gorka Ruiz; Acedo, Alberto; García-Casado, Zaida

    2014-01-01

    Rare sequence variants in "high-risk" disease genes, often referred as unclassified variants (UVs), pose a serious challenge to genetic testing. However, UVs resulting in splicing alterations can be readily assessed by in vitro assays. Unfortunately, analytical and clinical interpretation...... of these assays is often challenging. Here, we explore this issue by conducting splicing assays in 31 BRCA2 genetic variants. All variants were assessed by RT-PCR followed by capillary electrophoresis and direct sequencing. If assays did not produce clear-cut outputs (Class-2 or Class-5 according to analytical...... International Agency for Research on Cancer guidelines), we performed qPCR and/or minigene assays. The latter were performed with a new splicing vector (pSAD) developed by authors of the present manuscript (patent #P201231427 CSIC). We have identified three clinically relevant Class-5 variants (c.682-2A>G, c...

  9. Genomics of alternative splicing: evolution, development and pathophysiology.

    Science.gov (United States)

    Gamazon, Eric R; Stranger, Barbara E

    2014-06-01

    Alternative splicing is a major cellular mechanism in metazoans for generating proteomic diversity. A large proportion of protein-coding genes in multicellular organisms undergo alternative splicing, and in humans, it has been estimated that nearly 90 % of protein-coding genes-much larger than expected-are subject to alternative splicing. Genomic analyses of alternative splicing have illuminated its universal role in shaping the evolution of genomes, in the control of developmental processes, and in the dynamic regulation of the transcriptome to influence phenotype. Disruption of the splicing machinery has been found to drive pathophysiology, and indeed reprogramming of aberrant splicing can provide novel approaches to the development of molecular therapy. This review focuses on the recent progress in our understanding of alternative splicing brought about by the unprecedented explosive growth of genomic data and highlights the relevance of human splicing variation on disease and therapy.

  10. Tissue-specific splicing factor gene expression signatures

    NARCIS (Netherlands)

    A.R. Grosso; A.Q. Gomes (Anita); N.L. Barbosa-Morais (Nuno); S. Caldeira (Sandra); N.P. Thorne (Natalie); G. Grech (Godfrey); M.M. von Lindern (Marieke); M. Carmo-Fonseca (Maria)

    2008-01-01

    textabstractThe alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that

  11. Tissue-specific splicing factor gene expression signatures

    NARCIS (Netherlands)

    Grosso, Ana Rita; Gomes, Anita Q.; Barbosa-Morais, Nuno L.; Caldeira, Sandra; Thorne, Natalie P.; Grech, Godfrey; von Lindern, Marieke; Carmo-Fonseca, Maria

    2008-01-01

    The alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that tissue-specific

  12. Glass Fiber Reinforced Polymer Dowel Bar Evaluation

    Science.gov (United States)

    2012-09-01

    Glass Fiber Reinforced Polymer (GFRP) dowel bars were installed on one new construction project and two dowel bar : retrofit projects to evaluate the performance of this type of dowel bar in comparison to steel dowel bars installed on the same : cont...

  13. Field observations of nearshore bar formation

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian

    2008-01-01

      The formation of an inner nearshore bar was observed during a high-energy event at the sandy beach of Vejers, Denmark. The bar accreted in situ during surf zone conditions and the growth of the bar was associated with the development of a trough landward of the bar. Measurements of hydrodynamics...

  14. Comparison of splice sites in mammals and chicken

    OpenAIRE

    Abril Ferrando, Josep Francesc; Castelo Valdueza, Robert; Guigó Serra, Roderic

    2005-01-01

    We have carried out an initial analysis of the dynamics of the recent evolution of the splice-sites sequences on a large collection of human, rodent (mouse and rat), and chicken introns. Our results indicate that the sequences of splice sites are largely homogeneous within tetrapoda. We have also found that orthologous splice signals between human and rodents and within rodents are more conserved than unrelated splice sites, but the additional conservation can be explained mostly by backgroun...

  15. Optimization Model for Headway of a Suburban Bus Route

    Directory of Open Access Journals (Sweden)

    Xiaohong Jiang

    2014-01-01

    Full Text Available Due to relatively low passenger demand, headways of suburban bus route are usually longer than those of urban bus route. Actually it is also difficult to balance the benefits between passengers and operators, subject to the service standards from the government. Hence the headway of a suburban bus route is usually determined on the empirical experience of transport planners. To cope with this problem, this paper proposes an optimization model for designing the headways of suburban bus routes by minimizing the operating and user costs. The user costs take into account both the waiting time cost and the crowding cost. The feasibility and validity of the proposed model are shown by applying it to the Route 206 in Jiangning district, Nanjing city of China. Weightages of passengers’ cost and operating cost are further discussed, considering different passenger flows. It is found that the headway and objective function are affected by the weightages largely.

  16. Fixed Route Accessible Bus Service in Connecticut : A Case Study

    Science.gov (United States)

    1981-07-01

    U.S. Department of Transportation regulations implementing Section 504 of the Rehabilitation Act of 1973, require transit agencies to provide wheelchair accessibility on fixed-route bus service. The study is part of the series which included studies ...

  17. A research framework for studying transit bus driver distraction.

    Science.gov (United States)

    2013-09-01

    The increase in transit bus ridership nationally during the past ten years, along with the : proliferation of personal electronic control and communication gadgets is causing more : distractions for the drivers. Earlier research studies have found dr...

  18. Analysis of movable bus stop boarding and alighting areas.

    Science.gov (United States)

    2013-05-01

    This study explored the feasibility of using movable and reusable boarding and alighting (B&A) pads at bus stops. : Potential design alternatives in terms of materials and structural support for these pads were evaluated. The review : focused on the ...

  19. Multi-Gigabit Rate Radiation Hard Bus Project

    Data.gov (United States)

    National Aeronautics and Space Administration — High speed robust very low power radiation hardened bus interconnects are required to accelerate on-board computations, further improve reconfigurability and upgrade...

  20. FTA fuel cell bus program : research accomplishments through 2011.

    Science.gov (United States)

    2012-03-01

    Prepared by the Federal Transit Administration (FTA) Office of Research, Demonstration, and Innovation (TRI), this report summarizes the accomplishments of fuel-cell-transit-bus-related research and demonstrations projects supported by FTA through 20...

  1. Analysis of feeder bus network design and scheduling problems.

    Science.gov (United States)

    Almasi, Mohammad Hadi; Mirzapour Mounes, Sina; Koting, Suhana; Karim, Mohamed Rehan

    2014-01-01

    A growing concern for public transit is its inability to shift passenger's mode from private to public transport. In order to overcome this problem, a more developed feeder bus network and matched schedules will play important roles. The present paper aims to review some of the studies performed on Feeder Bus Network Design and Scheduling Problem (FNDSP) based on three distinctive parts of the FNDSP setup, namely, problem description, problem characteristics, and solution approaches. The problems consist of different subproblems including data preparation, feeder bus network design, route generation, and feeder bus scheduling. Subsequently, descriptive analysis and classification of previous works are presented to highlight the main characteristics and solution methods. Finally, some of the issues and trends for future research are identified. This paper is targeted at dealing with the FNDSP to exhibit strategic and tactical goals and also contributes to the unification of the field which might be a useful complement to the few existing reviews.

  2. Public transit: Bus, paratransit, and ridesharing. Transportation research record

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    Contents: part 1--bus operations--service reliability program of the southern california rapid transit district: a test of the short-term impacts on line 26-51; bus route o-d matrix generation: relationship between biproportional and recursive methods; transportation systems management options to improve urban bus route performance using computer simulation; development and application of performance measures for rural public transportation operators; rural public transportation in alaska: present and future options; particulate trap installation in a man articulated transit bus; part 2--paratransit--methodology for conducting a transportation survey of persons with disabilities; marketing rural transit among senior populations; evolution of functional eligibility and certification for paratransit service: the chicago experience; part 3--ridesharing--the state of the commute in southern california; hispanic market research in the southern california market; evaluation of second-year effectiveness of guaranteed ride home service at warner center transportation management organization; effectiveness of a statewide ridesharing promotion: california rideshare week.

  3. Dynamically possible pattern speeds of double bars

    OpenAIRE

    Maciejewski, Witold

    2009-01-01

    The method to study oscillating potentials of double bars, based on invariant loops, is introduced here in a new way, intended to be more intelligible. Using this method, I show how the orbital structure of a double-barred galaxy (nested bars) changes with the variation of nuclear bar's pattern speed. Not all pattern speeds are allowed when the inner bar rotates in the same direction as the outer bar. Below certain minimum pattern speed orbital support for the inner bar abruptly disappears, w...

  4. Application Layer Definition and Analyses of Controller Area Network Bus for Wire Harness Assembly Machine

    OpenAIRE

    Guo, Hui; Jiang, Ying

    2006-01-01

    With the feature of multi-master bus access, nondestructive contention-based arbitration and flexible configuration, Controller Area Network (CAN) bus is applied into the control system of Wire Harness Assembly Machine (WHAM). To accomplish desired goal, the specific features of the CAN bus is analyzed by compared with other field buses and the functional performances in the CAN bus system of WHAM is discussed. Then the application layer planning of CAN bus for dynamic priority is presented. ...

  5. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Penny, David

    2007-01-01

    , and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional...... classes, cellular locations, intron/exon structures and evolutionary origins. RESULTS: For each species, we find that genes from most functional categories are alternatively spliced. Ancient genes (shared between animals, fungi and plants) show high levels of alternative splicing. Genes with products...... expressed in the nucleus or plasma membrane are generally more alternatively spliced while those expressed in extracellular location show less alternative splicing. We find a clear correspondence between incidence of alternative splicing and intron number per gene both within and between genomes. In general...

  6. Accessibility analysis to public services by bus transportation

    OpenAIRE

    Košir, Uroš

    2009-01-01

    Graduation thesis focuses on ESRI GIS tools, Model Builder and Network analyst. It is shown how automated process with geoprocesing models simplify procedures, with prepared data, for further analyses. Data, established with analyses, were used for accessability analysis to public services by bus transportation. Analyses were made for territory of Slovenia and their statistical regions. For better understanding of choice between a car and a bus accessibility to public services, thesis was sup...

  7. VHDL resolved function based inner communication bus for FPGA

    Science.gov (United States)

    Pozniak, Krzysztof T.

    2017-08-01

    This article discusses a method of building an internal, universal and parametric bus. The solution was designed for a variety of FPGA families and popular VHDL compilers. The algorithm of automatic configuration of address space and methods of receiving and sending addressed data are discussed. The basic solution realized in VHDL language in a behavioral form and chosen examples of practical use of the internal bus are presented in detail.

  8. 30 CFR 77.504 - Electrical connections or splices; suitability.

    Science.gov (United States)

    2010-07-01

    ... UNDERGROUND COAL MINES Electrical Equipment-General § 77.504 Electrical connections or splices; suitability. Electrical connections or splices in electric conductors shall be mechanically and electrically efficient... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Electrical connections or splices; suitability...

  9. The implications of alternative splicing in the ENCODE protein complement

    DEFF Research Database (Denmark)

    Tress, Michael L.; Martelli, Pier Luigi; Frankish, Adam

    2007-01-01

    Alternative premessenger RNA splicing enables genes to generate more than one gene product. Splicing events that occur within protein coding regions have the potential to alter the biological function of the expressed protein and even to create new protein functions. Alternative splicing has been...

  10. School bus crash rates on routine and nonroutine routes.

    Science.gov (United States)

    O'Neal, Elizabeth; Ramirez, Marizen; Hamann, Cara; Young, Tracy; Stahlhut, Mary; Peek-Asa, Corinne

    2014-09-01

    Although prior research has established that school buses are a safe form of transportation, crashes can produce catastrophic consequences. School buses have 2 types of routes: predictable, routine routes that take children to and from school and less predictable, nonroutine routes for school events. No studies have examined school bus crash incidence and characteristics by these route types. School bus crashes were identified from the Iowa Department of Transportation Crash Database from mid-2005 through mid-2010. Crash reports did not identify whether the bus was on a routine or nonroutine route, so a protocol to assign these based on day and time was developed. Bus mileage was provided by the Iowa Department of Education. The school bus crash rate was 2.1 times higher on nonroutine routes than on routine routes (95% CI = 1.8-2.3). Most crashes involved an improper action by the driver of another vehicle. In crashes attributed to improper actions of school buses, failure to yield the right-of-way and disregarding traffic signs were more common on routine routes, while losing control, speeding, reckless, or aggressive driving were more common on nonroutine routes. School bus crashes are more likely to occur on nonroutine routes. © 2014, American School Health Association.

  11. Unhealthy and healthy weight control behaviours among bus operators.

    Science.gov (United States)

    Escoto, K H; French, S A

    2012-03-01

    Urban bus operators are an occupational group with high rates of overweight and obesity. Understanding methods bus operators use for weight control may be important; there may be increased risk for these workers to engage in less healthy weight management behaviours due to stressful working conditions. To examine the prevalence of unhealthy and healthy weight control behaviours used by bus operators and examine associations between use of unhealthy weight control behaviours and work-related and sociodemographic variables. Bus operators from four different transit garages were invited to complete a self-administered survey; height and weight were measured by research staff. Unhealthy and healthy weight control behaviours, work hours, work schedule and social support were measured with self-report items on the employee survey. Logistic regression analysis was conducted to estimate associations. Nearly 60% of bus operators endorsed at least one unhealthy method; over 50% reported skipping meals, 30% fasted and 10% reported taking diet pills in the past year. Bus operator gender, race, body mass index status and hours worked per week showed significant associations with using at least one unhealthy weight control behaviour. Worksite interventions should emphasize the benefit of healthy eating and physical activity but should also address the use of less healthy methods for weight control for individuals employed in transportation occupations.

  12. An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly.

    Science.gov (United States)

    Philippe, Lucas; Pandarakalam, George C; Fasimoye, Rotimi; Harrison, Neale; Connolly, Bernadette; Pettitt, Jonathan; Müller, Berndt

    2017-08-21

    Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the precise details of SL trans-splicing have yet to be elucidated. In part, this is because the systematic identification of the molecules involved has not previously been possible due to the lack of a specific phenotype associated with defects in this process. We present here a novel GFP-based reporter assay that can monitor SL1 trans-splicing in living C. elegans. Using this assay, we have identified mutants in sna-1 that are defective in SL trans-splicing, and demonstrate that reducing function of SNA-1, SNA-2 and SUT-1, proteins that associate with SL1 RNA and related SmY RNAs, impairs SL trans-splicing. We further demonstrate that the Sm proteins and pICln, SMN and Gemin5, which are involved in small nuclear ribonucleoprotein assembly, have an important role in SL trans-splicing. Taken together these results provide the first in vivo evidence for proteins involved in SL trans-splicing, and indicate that continuous replacement of SL ribonucleoproteins consumed during trans-splicing reactions is essential for effective trans-splicing. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  13. CROSS-FRONTIER PUBLIC TRANSPORT: Y BUS

    CERN Multimedia

    CERN Housing Service

    2002-01-01

    'Y' buses cover the journey between Val Thoiry in France (shopping centre including'Migros' and other stores) and Blandonnet in Switzerland, where they stop outside the 'Jumbo' shop, now renamed 'Carrefour'. In France, the buses connect up Thoiry, Sergy and St. Genis, then cross the border to stop outside CERN, Meyrin, before continuing towards Geneva along the 'Route de Meyrin', stopping outside the 'La Tour' hospital, then at the terminus Blandonnet. The normal fare on French territory with a slight incursion into Switzerland for this purpose as far as CERN, Meyrin, is 1.20 Euros per single journey (ticket valid for one hour). The same amount is charged for any single journey in Switzerland. For example, the cost of a single journey between St. Genis and the 'La Tour' hospital would be 2.40 Euros. The season ticket ('Abonnement de Travail') opens the right to purchase a ticket valid over any period of seven days which entitles the holder to as many journeys as required between the bus stop nearest to the pl...

  14. Conditional control of alternative splicing through light-triggered splice-switching oligonucleotides.

    Science.gov (United States)

    Hemphill, James; Liu, Qingyang; Uprety, Rajendra; Samanta, Subhas; Tsang, Michael; Juliano, Rudolph L; Deiters, Alexander

    2015-03-18

    The spliceosome machinery is composed of several proteins and multiple small RNA molecules that are involved in gene regulation through the removal of introns from pre-mRNAs in order to assemble exon-based mRNA containing protein-coding sequences. Splice-switching oligonucleotides (SSOs) are genetic control elements that can be used to specifically control the expression of genes through correction of aberrant splicing pathways. A current limitation with SSO methodologies is the inability to achieve conditional control of their function paired with high spatial and temporal resolution. We addressed this limitation through site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs. This enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing. The use of light as a regulatory element allows for tight spatial and temporal control of splice switching in mammalian cells and animals.

  15. DIMETHYL ETHER (DME)-FUELED SHUTTLE BUS DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Elana M. Chapman; Shirish Bhide; Jennifer Stefanik; Howard Glunt; Andre L. Boehman; Allen Homan; David Klinikowski

    2003-04-01

    The objectives of this research and demonstration program are to convert a campus shuttle bus to operation on dimethyl ether, a potential ultra-clean alternative diesel fuel. To accomplish this objective, this project includes laboratory evaluation of a fuel conversion strategy, as well as, field demonstration of the DME-fueled shuttle bus. Since DME is a fuel with no lubricity (i.e., it does not possess the lubricating quality of diesel fuel), conventional fuel delivery and fuel injection systems are not compatible with dimethyl ether. Therefore, to operate a diesel engine on DME one must develop a fuel-tolerant injection system, or find a way to provide the necessary lubricity to the DME. In this project, they have chosen the latter strategy in order to achieve the objective with minimal need to modify the engine. Their strategy is to blend DME with diesel fuel, to obtain the necessary lubricity to protect the fuel injection system and to achieve low emissions. The bulk of the efforts over the past year were focused on the conversion of the campus shuttle bus. This process, started in August 2001, took until April 2002 to complete. The process culminated in an event to celebrate the launching of the shuttle bus on DME-diesel operation on April 19, 2002. The design of the system on the shuttle bus was patterned after the system developed in the engine laboratory, but also was subjected to a rigorous failure modes effects analysis (FMEA, referred to by Air Products as a ''HAZOP'' analysis) with help from Dr. James Hansel of Air Products. The result of this FMEA was the addition of layers of redundancy and over-pressure protection to the system on the shuttle bus. The system became operational in February 2002. Preliminary emissions tests and basic operation of the shuttle bus took place at the Pennsylvania Transportation Institute's test track facility near the University Park airport. After modification and optimization of the system on

  16. Star Formation Activity of Barred Spiral Galaxies

    Science.gov (United States)

    Kim, Eunbin; Hwang, Ho Seong; Chung, Haeun; Lee, Gwang-Ho; Park, Changbom; Cervantes Sodi, Bernardo; Kim, Sungsoo S.

    2017-08-01

    We study the star formation activity of nearby galaxies with bars using a sample of late-type galaxies at 0.02≤slant z≤slant 0.05489 and {M}rgas in strongly barred galaxies are smaller than those in non-barred galaxies, and the gas metallicity is higher in strongly barred galaxies than in non-barred galaxies. The gas properties of weakly barred galaxies again show no difference from those of non-barred galaxies. We stack the optical spectra of barred and non-barred galaxies in several mass bins and fit to the stacked spectra with a spectral fitting code, STARLIGHT. We find no significant difference in stellar populations between barred and non-barred galaxies for both strongly and weakly barred galaxies. Our results are consistent with the idea that the star formation activity of barred galaxies was enhanced in the past along with significant gas consumption, and is currently lower than or similar to that of non-barred galaxies. The past star formation enhancement depends on the strength of bars.

  17. Auxiliary splice factor U2AF26 and transcription factor Gfi1 cooperate directly in regulating CD45 alternative splicing.

    NARCIS (Netherlands)

    Heyd, F.; Dam, G.B. ten; Moroy, T.

    2006-01-01

    By alternative splicing, different isoforms of the transmembrane tyrosine phosphatase CD45 are generated that either enhance or limit T cell receptor signaling. We report here that CD45 alternative splicing is regulated by cooperative action of the splice factor U2AF26 and the transcription factor

  18. A Rule-Based Energy Management Strategy for a Plug-in Hybrid School Bus Based on a Controller Area Network Bus

    National Research Council Canada - National Science Library

    Peng, Jiankun; Fan, Hao; He, Hongwen; Pan, Deng

    2015-01-01

    .... To evaluate the controller area network (CAN) bus performance features such as the bus load, signal hysteresis, and to verify the reliability and real-time performance of the CAN bus multi-node control method, a co-simulation platform was built...

  19. An Improved Model for Headway-Based Bus Service Unreliability Prevention with Vehicle Load Capacity Constraint at Bus Stops

    Directory of Open Access Journals (Sweden)

    Weiya Chen

    2012-01-01

    Full Text Available This paper presents an improved model for improving headway-based bus route service reliability at bus stops using real-time preventive operation control, taking into account dynamic interaction among random passenger demand, stochastic driving conditions of route segments, and vehicle load capacity constraint. In this model, the real-time information of passenger demand and vehicle operation is involved to predict the imminent unacceptable headway deviation, in the case of which some in-time preventive control strategies are deployed according to the given control rules. As a case study, a single fixed bus route with high-frequency services was simulated and different scenarios of real-time preventive operation control were performed. Headway adherence and average passenger wait time were used to measure bus service reliability. The results show that the improved model is closer to the real bus route service, and using real-time information to predict potential service unreliability and trigger in-time preventive control can reduce bus bunching and avoid big gap.

  20. On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.

    Science.gov (United States)

    Krchňáková, Zuzana; Krajčovič, Juraj; Vesteg, Matej

    2017-08-01

    Trans-splicing is a process by which 5'- and 3'-ends of two pre-RNA molecules transcribed from different sites of the genome can be joined together to form a single RNA molecule. The spliced leader (SL) trans-splicing is mediated by the spliceosome and it allows the replacement of 5'-end of pre-mRNA by 5'(SL)-end of SL-RNA. This form of splicing has been observed in many phylogenetically unrelated eukaryotes. Either the SL trans-splicing (SLTS) originated in the last eukaryotic common ancestor (LECA) (or even earlier) and it was lost in most eukaryotic lineages, or this mechanism of RNA processing evolved several times independently in various unrelated eukaryotic taxa. The bioinformatic comparisons of SL-RNAs from various eukaryotic taxonomic groups have revealed the similarities of secondary structures of most SL-RNAs and a relative conservation of their splice sites (SSs) and Sm-binding sites (SmBSs). We propose that such structural and functional similarities of SL-RNAs are unlikely to have evolved repeatedly many times. Hence, we favor the scenario of an early evolutionary origin for the SLTS and multiple losses of SL-RNAs in various eukaryotic lineages.

  1. Survey of gene splicing algorithms based on reads.

    Science.gov (United States)

    Si, Xiuhua; Wang, Qian; Zhang, Lei; Wu, Ruo; Ma, Jiquan

    2017-09-05

    Gene splicing is the process of assembling a large number of unordered short sequence fragments to the original genome sequence as accurately as possible. Several popular splicing algorithms based on reads are reviewed in this article, including reference genome algorithms and de novo splicing algorithms (Greedy-extension, Overlap-Layout-Consensus graph, De Bruijn graph). We also discuss a new splicing method based on the MapReduce strategy and Hadoop. By comparing these algorithms, some conclusions are drawn and some suggestions on gene splicing research are made.

  2. ANALISIS SARANA ANGKUTAN UMUM BUS DAMRI DI KOTA SEMARANG

    Directory of Open Access Journals (Sweden)

    Subhan Rizal Amin

    2015-12-01

    Full Text Available This study aims to analyze the availability and use of public transportation by taking a case study of the Damri bus which has Ngaliyan-Pucanggading route. The availability of public transport is not comparable to the demand for public transportation services so that the problems of transportation emerge. Additionally, the use of public transport becomes an important factor to develop the transportation. The study is conducted by surveying the Damri passengers of B.04 lane and logistic regression is used for the analysis. The method of determining the needs of transportation is issued by Departemen Perhubungan. It is used to calculate and determine whether the public transportation should be added or reduced. Then, the factors used as the independent variables in the analysis are the Damri’s safety, effectiveness, efficiency, service and reliability. The results show that Perum Damri does not need to add more Damri bus for B.04 because the results’ calculation is 0.6. and the factors that significantly influence the use Damri bus is the variable of safety, effectiveness and efficiency. However, the variables of service and reliability do not significantly influence the use of B.04 Damri bus.Penelitian ini bertujuan untuk menganalisis ketersediaan dan penggunaan angkutan sarana angkutan umum penumpang dengan mengambil studi kasus pada bus Damri jalur dengan trayek Ngaliyan-Pucanggading. Ketersediaan sarana angkutan umum yang tersedia tidak sebanding dengan besarnya permintaan akan jasa angkutan umum oleh masyarakat umum sehingga muncul permasalahan transportasi. Di samping itu, penggunaan angkutan umum yang beroperasi menjadi faktor penting dalam upaya pengembangan sarana transportasi. Penelitian ini dilakukan dengan menggunakan metode survey terhadap para penumpang bus Damri jalur B.04 dan dianalisis dengan Regresi Logistik. Metode penentuan kebutuhan armada dikeluarkan oleh Departemen Perhubungan yang digunakan untuk menghitung apakah suatu

  3. Iterated Local Search Algorithm with Strategic Oscillation for School Bus Routing Problem with Bus Stop Selection

    Directory of Open Access Journals (Sweden)

    Mohammad Saied Fallah Niasar

    2017-02-01

    Full Text Available he school bus routing problem (SBRP represents a variant of the well-known vehicle routing problem. The main goal of this study is to pick up students allocated to some bus stops and generate routes, including the selected stops, in order to carry students to school. In this paper, we have proposed a simple but effective metaheuristic approach that employs two features: first, it utilizes large neighborhood structures for a deeper exploration of the search space; second, the proposed heuristic executes an efficient transition between the feasible and infeasible portions of the search space. Exploration of the infeasible area is controlled by a dynamic penalty function to convert the unfeasible solution into a feasible one. Two metaheuristics, called N-ILS (a variant of the Nearest Neighbourhood with Iterated Local Search algorithm and I-ILS (a variant of Insertion with Iterated Local Search algorithm are proposed to solve SBRP. Our experimental procedure is based on the two data sets. The results show that N-ILS is able to obtain better solutions in shorter computing times. Additionally, N-ILS appears to be very competitive in comparison with the best existing metaheuristics suggested for SBRP

  4. Bar-spheroid interaction in galaxies

    Science.gov (United States)

    Hernquist, Lars; Weinberg, Martin D.

    1992-01-01

    N-body simulation and linear analysis is employed to investigate the secular evolution of barred galaxies, with emphasis on the interaction between bars and spheroidal components of galaxies. This interaction is argued to drive secular transfer of angular momentum from bars to spheroids, primarily through resonant coupling. A moderately strong bar, having mass within corotation about 0.3 times the enclosed spheroid mass, is predicted to shed all its angular momentum typically in less than about 10 exp 9 yr. Even shorter depletion time scales are found for relatively more massive bars. It is suggested either that spheroids around barred galaxies are structured so as to inhibit strong coupling with bars, or that bars can form by unknown processes long after disks are established. The present models reinforce the notion that bars can drive secular evolution in galaxies.

  5. Data Rates Assessment on L2–L3 CPU Bus and Bus between CPU and RAM in Modern CPUs

    Directory of Open Access Journals (Sweden)

    Maria S. Komar

    2017-01-01

    Full Text Available In this paper, a modern CPU architecture with several different cache levels is described, and current CPU performance limitations such as silicone physical limitations or frequency increase bounds are mentioned. As usual, changes of the currently existing architecture are proposed as a way of increasing CPU performance, data rates on the internal and external CPU interfaces must be known. It would help to assess applicability of proposed solutions and allow to optimize them. This paper is aimed at getting real values of traffic on L2-L3 cache interface inside CPU and CPU-RAM bus load as well as show dependencies of total traffic on the interfaces of interest on the number of active cores, CPU frequency and test type. Measurements methodology using Intel Performance Counter Monitor by Intel is provided and equations that allow to get data rates from internal CPU counters are explained. Both real life and synthetic tests are described. Dependency of total traffic on the number of active cores and dependency of total traffic on CPU frequency are provided as plots. Dependency of total traffic on test type provided as bar plot for multiple CPU frequencies.

  6. Computational prediction of splicing regulatory elements shared by Tetrapoda organisms

    Science.gov (United States)

    Churbanov, Alexander; Vořechovský, Igor; Hicks, Chindo

    2009-01-01

    Background Auxiliary splicing sequences play an important role in ensuring accurate and efficient splicing by promoting or repressing recognition of authentic splice sites. These cis-acting motifs have been termed splicing enhancers and silencers and are located both in introns and exons. They co-evolved into an intricate splicing code together with additional functional constraints, such as tissue-specific and alternative splicing patterns. We used orthologous exons extracted from the University of California Santa Cruz multiple genome alignments of human and 22 Tetrapoda organisms to predict candidate enhancers and silencers that have reproducible and statistically significant bias towards annotated exonic boundaries. Results A total of 2,546 Tetrapoda enhancers and silencers were clustered into 15 putative core motifs based on their Markov properties. Most of these elements have been identified previously, but 118 putative silencers and 260 enhancers (~15%) were novel. Examination of previously published experimental data for the presence of predicted elements showed that their mutations in 21/23 (91.3%) cases altered the splicing pattern as expected. Predicted intronic motifs flanking 3' and 5' splice sites had higher evolutionary conservation than other sequences within intronic flanks and the intronic enhancers were markedly differed between 3' and 5' intronic flanks. Conclusion Difference in intronic enhancers supporting 5' and 3' splice sites suggests an independent splicing commitment for neighboring exons. Increased evolutionary conservation for ISEs/ISSs within intronic flanks and effect of modulation of predicted elements on splicing suggest functional significance of found elements in splicing regulation. Most of the elements identified were shown to have direct implications in human splicing and therefore could be useful for building computational splicing models in biomedical research. PMID:19889216

  7. Computational prediction of splicing regulatory elements shared by Tetrapoda organisms

    Directory of Open Access Journals (Sweden)

    Hicks Chindo

    2009-11-01

    Full Text Available Abstract Background Auxiliary splicing sequences play an important role in ensuring accurate and efficient splicing by promoting or repressing recognition of authentic splice sites. These cis-acting motifs have been termed splicing enhancers and silencers and are located both in introns and exons. They co-evolved into an intricate splicing code together with additional functional constraints, such as tissue-specific and alternative splicing patterns. We used orthologous exons extracted from the University of California Santa Cruz multiple genome alignments of human and 22 Tetrapoda organisms to predict candidate enhancers and silencers that have reproducible and statistically significant bias towards annotated exonic boundaries. Results A total of 2,546 Tetrapoda enhancers and silencers were clustered into 15 putative core motifs based on their Markov properties. Most of these elements have been identified previously, but 118 putative silencers and 260 enhancers (~15% were novel. Examination of previously published experimental data for the presence of predicted elements showed that their mutations in 21/23 (91.3% cases altered the splicing pattern as expected. Predicted intronic motifs flanking 3' and 5' splice sites had higher evolutionary conservation than other sequences within intronic flanks and the intronic enhancers were markedly differed between 3' and 5' intronic flanks. Conclusion Difference in intronic enhancers supporting 5' and 3' splice sites suggests an independent splicing commitment for neighboring exons. Increased evolutionary conservation for ISEs/ISSs within intronic flanks and effect of modulation of predicted elements on splicing suggest functional significance of found elements in splicing regulation. Most of the elements identified were shown to have direct implications in human splicing and therefore could be useful for building computational splicing models in biomedical research.

  8. Computational prediction of splicing regulatory elements shared by Tetrapoda organisms.

    Science.gov (United States)

    Churbanov, Alexander; Vorechovský, Igor; Hicks, Chindo

    2009-11-04

    Auxiliary splicing sequences play an important role in ensuring accurate and efficient splicing by promoting or repressing recognition of authentic splice sites. These cis-acting motifs have been termed splicing enhancers and silencers and are located both in introns and exons. They co-evolved into an intricate splicing code together with additional functional constraints, such as tissue-specific and alternative splicing patterns. We used orthologous exons extracted from the University of California Santa Cruz multiple genome alignments of human and 22 Tetrapoda organisms to predict candidate enhancers and silencers that have reproducible and statistically significant bias towards annotated exonic boundaries. A total of 2,546 Tetrapoda enhancers and silencers were clustered into 15 putative core motifs based on their Markov properties. Most of these elements have been identified previously, but 118 putative silencers and 260 enhancers (~15%) were novel. Examination of previously published experimental data for the presence of predicted elements showed that their mutations in 21/23 (91.3%) cases altered the splicing pattern as expected. Predicted intronic motifs flanking 3' and 5' splice sites had higher evolutionary conservation than other sequences within intronic flanks and the intronic enhancers were markedly differed between 3' and 5' intronic flanks. Difference in intronic enhancers supporting 5' and 3' splice sites suggests an independent splicing commitment for neighboring exons. Increased evolutionary conservation for ISEs/ISSs within intronic flanks and effect of modulation of predicted elements on splicing suggest functional significance of found elements in splicing regulation. Most of the elements identified were shown to have direct implications in human splicing and therefore could be useful for building computational splicing models in biomedical research.

  9. Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern.

    Directory of Open Access Journals (Sweden)

    Clément Bouton

    Full Text Available The plant pararetrovirus Cauliflower mosaic virus (CaMV uses alternative splicing to generate several isoforms from its polycistronic pregenomic 35S RNA. This pro-cess has been shown to be essential for infectivity. Previous works have identified four splice donor sites and a single splice acceptor site in the 35S RNA 5' region and suggested that the main role of CaMV splicing is to downregulate expression of open reading frames (ORFs I and II. In this study, we show that alternative splicing is a conserved process among CaMV isolates. In Cabb B-JI and Cabb-S isolates, splicing frequently leads to different fusion between ORFs, particularly between ORF I and II. The corresponding P1P2 fusion proteins expressed in E. coli interact with viral proteins P2 and P3 in vitro. However, they are detected neither during infection nor upon transient expression in planta, which suggests rapid degradation after synthesis and no important biological role in the CaMV infectious cycle. To gain a better understanding of the functional relevance of 35S RNA alternative splicing in CaMV infectivity, we inactivated the previously described splice sites. All the splicing mutants were as pathogenic as the corresponding wild-type isolate. Through RT-PCR-based analysis we demonstrate that CaMV 35S RNA exhibits a complex splicing pattern, as we identify new splice donor and acceptor sites whose selection leads to more than thirteen 35S RNA isoforms in infected turnip plants. Inactivating splice donor or acceptor sites is not lethal for the virus, since disrupted sites are systematically rescued by the activation of cryptic and/or seldom used splice sites. Taken together, our data depict a conserved, complex and flexible process, involving multiple sites, that ensures splicing of 35S RNA.

  10. Orbital Support of Fast and Slow Inner Bars in Double Barred Galaxies

    OpenAIRE

    Maciejewski, Witold; Small, Emma E.

    2010-01-01

    We analyze how the orbital support of the inner bar in a double-barred galaxy (nested bars) depends on the angular velocity (i.e. pattern speed) of this bar. We study orbits in seven models of double bars using the method of invariant loops. The range of pattern speed is covered exhaustively. We find that not all pattern speeds are allowed when the inner bar rotates in the same direction as the outer bar. Below a certain minimum pattern speed orbital support for the inner bar abruptly disappe...

  11. Development of a novel splice array platform and its application in the identification of alternative splice variants in lung cancer

    Directory of Open Access Journals (Sweden)

    Gomez-Roman Javier

    2010-06-01

    Full Text Available Abstract Background Microarrays strategies, which allow for the characterization of thousands of alternative splice forms in a single test, can be applied to identify differential alternative splicing events. In this study, a novel splice array approach was developed, including the design of a high-density oligonucleotide array, a labeling procedure, and an algorithm to identify splice events. Results The array consisted of exon probes and thermodynamically balanced junction probes. Suboptimal probes were tagged and considered in the final analysis. An unbiased labeling protocol was developed using random primers. The algorithm used to distinguish changes in expression from changes in splicing was calibrated using internal non-spliced control sequences. The performance of this splice array was validated with artificial constructs for CDC6, VEGF, and PCBP4 isoforms. The platform was then applied to the analysis of differential splice forms in lung cancer samples compared to matched normal lung tissue. Overexpression of splice isoforms was identified for genes encoding CEACAM1, FHL-1, MLPH, and SUSD2. None of these splicing isoforms had been previously associated with lung cancer. Conclusions This methodology enables the detection of alternative splicing events in complex biological samples, providing a powerful tool to identify novel diagnostic and prognostic biomarkers for cancer and other pathologies.

  12. Observations of offshore bar decay

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian

    2010-01-01

    the upper shoreface, and finally a stage of decaying bar form through loss of sediment volume at the outer boundary of the upper shoreface. The phenomenon has been previously documented in the Netherlands, the USA, the Canadian Great Lakes, and in New Zealand, but our present understanding...

  13. Hidden bars and boxy bulges

    NARCIS (Netherlands)

    Merrifield, MR; Kuijken, K

    It has been suggested that the boxy and peanut-shaped bulges found in some edge-on galaxies are galactic bars viewed from the side. We investigate this hypothesis by presenting emission-line spectra for a sample of 10 edge-on galaxies that display a variety of bulge morphologies. To avoid potential

  14. Global genome splicing analysis reveals an increased number of alternatively spliced genes with aging.

    Science.gov (United States)

    Rodríguez, Sofía A; Grochová, Diana; McKenna, Tomás; Borate, Bhavesh; Trivedi, Niraj S; Erdos, Michael R; Eriksson, Maria

    2016-04-01

    Alternative splicing (AS) is a key regulatory mechanism for the development of different tissues; however, not much is known about changes to alternative splicing during aging. Splicing events may become more frequent and widespread genome-wide as tissues age and the splicing machinery stringency decreases. Using skin, skeletal muscle, bone, thymus, and white adipose tissue from wild-type C57BL6/J male mice (4 and 18 months old), we examined the effect of age on splicing by AS analysis of the differential exon usage of the genome. The results identified a considerable number of AS genes in skeletal muscle, thymus, bone, and white adipose tissue between the different age groups (ranging from 27 to 246 AS genes corresponding to 0.3-3.2% of the total number of genes analyzed). For skin, skeletal muscle, and bone, we included a later age group (28 months old) that showed that the number of alternatively spliced genes increased with age in all three tissues (P aging disease Hutchinson-Gilford progeria syndrome was performed. The results show that expression of the mutant protein, progerin, is associated with an impaired developmental splicing. As progerin accumulates, the number of genes with AS increases compared to in wild-type skin. Our results indicate the existence of a mechanism for increased AS during aging in several tissues, emphasizing that AS has a more important role in the aging process than previously known. © 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  15. Deciphering the plant splicing code: Experimental and computational approaches for predicting alternative splicing and splicing regulatory elements

    Directory of Open Access Journals (Sweden)

    Anireddy S.N. Reddy

    2012-02-01

    Full Text Available Extensive alternative splicing (AS of precursor mRNAs (pre-mRNAs in multicellular eukaryotes increases the protein-coding capacity of a genome and allows novel ways to regulate gene expression. In fowering plants, up to 48% of intron-containing genes exhibit AS. However, the full extent of AS in plants is not yet known, as only a few high throughput RNA-Seq studies have been performed. As the cost of obtaining RNA-Seq reads continues to fall, it is anticipated that huge amounts of plant sequence data will accumulate and help in obtaining a more complete picture of AS in plants. Although it is not an onerous task to obtain hundreds of millions of reads using high throughput sequencing technologies, computational tools to accurately predict and visualize AS are still being developed and refined. This review will discuss the tools to predict and visualize transcriptome-wide AS in plants using short reads and highlight their limitations. Comparative studies of AS events between plants and animals have revealed that there are major differences in the most prevalent types of AS events, suggesting that plants and animals differ in the way they recognize exons and introns. Extensive studies have been performed in animals to identify cis-elements involved in regulating AS, especially in exon skipping. However, such studies are in their infancy in plants. Here, we review the current state of research on splicing regulatory elements (SREs and briefly discuss emerging experimental and computational tools to identify cis-elements involved in regulation of AS in plants. The availability of curated alternative splice forms in plants makes it possible to use computational tools to predict SREs involved in AS regulation, which can then be verified experimentally. Such studies will permit identification of plant-specific features involved in AS regulation and contribute to deciphering the splicing code in plants.

  16. Graz, city bus fleet running on 100% biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Ablasser, G. [City of Graz (Austria)

    2008-07-01

    Graz collects used cooking oil on a large scale. This used cooking oil is converted into biodiesel and along with biodiesel produced from rape seed provides the whole bus fleet (135 buses) with fuel. The bus fleet also got an optimized high quality interior design (information screens, air conditioning, a better acoustic and visual information and ramps for mobility-impaired persons). Attractiveness of the city will increase due to lower pollution levels by the biodiesel driven buses. Public transport became more user-friendly, especially for the disabled and other specific groups. The local public transport company, GBV, carries out a series of technical, ecological and economic trials of 100% biodiesel operation in the meantime also during wintertime. Within the framework of CIVITAS trendsetter, the whole bus fleet was either converted to biodiesel operation or new buses were bought already equipped for biodiesel operations. The 100% switch to alternative fuel for the entire bus fleet of the City of Graz is new; Graz is the only city in the world operating the whole bus fleet on 100% biodiesel.

  17. Shift work and arteriosclerosis risk in professional bus drivers.

    Science.gov (United States)

    Chen, Chun-Chieh; Shiu, Li-Jie; Li, Yu-Ling; Tung, Kuan-Yeng; Chan, Kwan-Yu; Yeh, Chih-Jung; Chen, Shiuan-Chih; Wong, Ruey-Hong

    2010-01-01

    Professional bus drivers are at increased risk for cardiovascular disease, but the underlying causes are unclear. Professional bus drivers often follow shift schedules. Especially, an association between shift work and early manifestations of cardiovascular disease has not been elucidated. Thus we investigated the links between shift work and arteriosclerosis risk in professional bus drivers. Questionnaires were administered to 184 bus drivers on demographic characteristics, lifestyle, and occupational history from 5 transportation companies in Taiwan. Brachial-ankle pulse wave velocity (baPWV) was measured using a volume-plethysmographic apparatus. Body mass index, waist circumference, biochemical variables, and blood pressure were also measured. Arteriosclerotic risk factors (age, weekly driving hours, systolic blood pressure, diastolic blood pressure, and insulin level) differed in part among different groups of drivers. Long-term shift drivers had higher baPWV compared to regular drivers and short-term shift drivers (1594 cm/s vs. 1497 and 1432, pshift driving. Long-term shift work could increase the risk of arteriosclerosis in professional bus drivers. Larger studies would be necessary to provide further evidence regarding this finding.

  18. Bus transport network of Shenyang considering competitive and cooperative relationship

    Science.gov (United States)

    Hu, Baoyu; Feng, Shumin; Nie, Cen

    2017-01-01

    Competition and cooperation is a universal phenomenon in bus transport networks (BTNs) because of the shared stations between bus routes. A measuring method is proposed for competitive and cooperative relationship between bus routes. Based on this measurement, we develop a new representation model for BTNs, namely competitive-cooperative space R. This model is applied to investigate empirically bus transport network of Shenyang (BTN-S) from China. We present the histograms of competitive-cooperative coefficients, competitive coefficients and cooperative coefficients to illustrate that competitive and cooperative relationship plays an important role in transporting passengers. The competitive-cooperative situation shows that cooperative relationship holds an absolutely dominant position in BTN-S. To explore the networked characteristics, we present some empirical distributions, for the number of bus stations on a route, the number of shared stations between two routes, degree and weighted degree, competitive strength, and cooperative strength. We also examine the correlations between degree and competitive strength, and between degree and cooperative strength. Besides, we investigate the diversities of competitive strength and cooperative strength in BTN-S. This study can help us to understand the BTN from a deeper level.

  19. Bus network redesign for inner southeast suburbs of Melbourne, Australia

    Science.gov (United States)

    Pandangwati, S. T.; Milyanab, N. A.

    2017-06-01

    Public transport is the most effective mode of transport in the era of climate change and oil depletion. It can address climate change issues by reducing urban greenhouse gas emission and oil consumption while at the same time improving mobility. However, many public transport networks are not effective and instead create high operating costs with low frequencies and occupancy. Melbourne is one example of a metropolitan area that faces this problem. Even though the city has well-integrated train and tram networks, Melbourne’s bus network still needs to be improved. This study used network planning approach to redesign the bus network in the City of Glen Eira, a Local Government Area (LGA) in the southeastern part of Metropolitan Melbourne. The study area is the area between Gardenvale North and Oakleigh Station, as well as between Caulfield and Patterson Stations. This area needs network improvement mainly because of the meandering bus routes that run within it. This study aims to provide recommendations for improving the performance of bus services by reducing meandering routes, improving transfer point design and implementing coordinated timetables. The recommendations were formulated based on a ‘ready-made’ concept to increase bus occupancy. This approach can be implemented in other cities with similar problems and characteristics including those in Indonesia.

  20. Car Delay Model near Bus Stops with Mixed Traffic Flow

    Directory of Open Access Journals (Sweden)

    Yang Xiaobao

    2013-01-01

    Full Text Available This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the stopping buses. The second occurs as buses merge back to the motorized lane. The average car delay is estimated as the sum of the average delay at these two conflict points and the delay resulting from following the slower bicycles that merged into the motorized lane. Data are collected to calibrate and validate the developed model from one site in Beijing. The sensitivity of car delay to various operation conditions is examined. The results show that both bus stream and bicycle stream have significant effects on car delay. At bus volumes above 200 vehicles per hour, the curbside stop design is not appropriate because of the long car delays. It can be replaced by the bus bay design.

  1. Genomic HEXploring allows landscaping of novel potential splicing regulatory elements.

    Science.gov (United States)

    Erkelenz, Steffen; Theiss, Stephan; Otte, Marianne; Widera, Marek; Peter, Jan Otto; Schaal, Heiner

    2014-01-01

    Effective splice site selection is critically controlled by flanking splicing regulatory elements (SREs) that can enhance or repress splice site use. Although several computational algorithms currently identify a multitude of potential SRE motifs, their predictive power with respect to mutation effects is limited. Following a RESCUE-type approach, we defined a hexamer-based 'HEXplorer score' as average Z-score of all six hexamers overlapping with a given nucleotide in an arbitrary genomic sequence. Plotted along genomic regions, HEXplorer score profiles varied slowly in the vicinity of splice sites. They reflected the respective splice enhancing and silencing properties of splice site neighborhoods beyond the identification of single dedicated SRE motifs. In particular, HEXplorer score differences between mutant and reference sequences faithfully represented exonic mutation effects on splice site usage. Using the HIV-1 pre-mRNA as a model system highly dependent on SREs, we found an excellent correlation in 29 mutations between splicing activity and HEXplorer score. We successfully predicted and confirmed five novel SREs and optimized mutations inactivating a known silencer. The HEXplorer score allowed landscaping of splicing regulatory regions, provided a quantitative measure of mutation effects on splice enhancing and silencing properties and permitted calculation of the mutationally most effective nucleotide. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. Alternative Splicing and Subfunctionalization Generates Functional Diversity in Fungal Proteomes

    Science.gov (United States)

    Jiménez-López, Claudia; Lorenz, Michael C.; van Hoof, Ambro

    2013-01-01

    Alternative splicing is commonly used by the Metazoa to generate more than one protein from a gene. However, such diversification of the proteome by alternative splicing is much rarer in fungi. We describe here an ancient fungal alternative splicing event in which these two proteins are generated from a single alternatively spliced ancestral SKI7/HBS1 gene retained in many species in both the Ascomycota and Basidiomycota. While the ability to express two proteins from a single SKI7/HBS1 gene is conserved in many fungi, the exact mechanism by which they achieve this varies. The alternative splicing was lost in Saccharomyces cerevisiae following the whole-genome duplication event as these two genes subfunctionalized into the present functionally distinct HBS1 and SKI7 genes. When expressed in yeast, the single gene from Lachancea kluyveri generates two functionally distinct proteins. Expression of one of these proteins complements hbs1, but not ski7 mutations, while the other protein complements ski7, but not hbs1. This is the first known case of subfunctionalization by loss of alternative splicing in yeast. By coincidence, the ancestral alternatively spliced gene was also duplicated in Schizosaccharomyces pombe with subsequent subfunctionalization and loss of splicing. Similar subfunctionalization by loss of alternative splicing in fungi also explains the presence of two PTC7 genes in the budding yeast Tetrapisispora blattae, suggesting that this is a common mechanism to preserve duplicate alternatively spliced genes. PMID:23516382

  3. Spliced leader RNA-mediated trans-splicing in phylum Rotifera.

    Science.gov (United States)

    Pouchkina-Stantcheva, Natalia N; Tunnacliffe, Alan

    2005-06-01

    In kinetoplastids, Euglena, and four metazoan phyla, trans-splicing has been described as a mechanism for the generation of mature messenger RNAs (mRNAs): 5'-ends of precursor mRNAs are replaced by a short spliced leader (SL) exon from a small SL RNA. Although the full phylogenetic range is unknown, trans-splicing has not been found in vertebrates, insects, plants, or yeast. In animal groups where it does occur, i.e., nematodes, cnidarians, platyhelminths, and primitive chordates, SL RNAs do not show sequence relatedness across phyla. The apparently sporadic phylogenetic distribution and the lack of SL RNA homology have led to opposing hypotheses on its evolution, involving either an ancient origin followed by loss in multiple lineages or independent acquisition in several taxa. Here we present evidence for the occurrence of trans-splicing in bdelloid rotifers (Bdelloidea, Rotifera). A common 23-nt sequence, representing the SL exon-diagnostic of SL RNA-mediated trans-splicing-was found at the 5'-end of at least 50%-65% of mRNAs from Adineta ricciae and Philodina sp. The trans-splicing pattern in bdelloid rotifers can be unusually complex, as observed in transcripts from a heat shock protein gene, hsp82-1, where the SL exon was spliced to three alternative positions. Bdelloid rotifer SL RNAs were found to be 105 or 106 nt long and comprised the SL sequence, a conserved splice donor site and an intron containing a putative spliceosome-binding motif. Intriguingly, some similarity of rotifer SL RNA sequence and predicted secondary structure was seen to that of the predominant SL1 RNA of nematodes, although it is unlikely that this demonstrates homology. In addition, sequence corresponding to the rotifer SL exon was found at the 5'-end of a number of full-length complementary DNA (cDNA) clones in a rice (Oryza sativa) database. None of these cDNAs gave a close match with homologous plant genes, suggesting that a small but significant portion of the rice expressed

  4. Going Smokefree Matters - Bars and Restaurants Infographic

    Data.gov (United States)

    U.S. Department of Health & Human Services — Explore the Going Smokefree Matters – Bars and Restaurants Infographic which outlines key facts related to the effects of secondhand smoke exposure in bars and...

  5. DNA splicing by directed ligation (SDL).

    Science.gov (United States)

    Berlin, Y A

    1999-01-01

    Splicing by directed ligation (SDL) is a method of in-phase joining of PCR-generated DNA fragments that is based on a pre-designed combination of class IIS restriction endonuclease recognition and cleavage sites. Since these enzymes cleave outside of their recognition sites, the resulting sticky end can have any desired sequence, and the site itself can be removed and does not appear in the final spliced DNA product. SDL is based on the addition of class IIS recognition sites onto primers used to amplify DNA sequences. Cleavage of the PCR products results in elimination of the recognition site-containing flanking sequences and leaves the DNA fragments crowned with protruding ends. With careful design of the sticky ends, several segments can be ligated together in a predetermined order in a single reaction. SDL requires fewer rounds of amplification than overlap extension methods, and is particularly useful for creating a series of recombinants that differ in one segment.

  6. Intravitreal Injection of Splice-switching Oligonucleotides to Manipulate Splicing in Retinal Cells

    Directory of Open Access Journals (Sweden)

    Xavier Gérard

    2015-01-01

    Full Text Available Leber congenital amaurosis is a severe hereditary retinal dystrophy responsible for neonatal blindness. The most common disease-causing mutation (c.2991+1655A>G; 10–15% creates a strong splice donor site that leads to insertion of a cryptic exon encoding a premature stop codon. Recently, we reported that splice-switching oligonucleotides (SSO allow skipping of the mutant cryptic exon and the restoration of ciliation in fibroblasts of affected patients, supporting the feasibility of a SSO-mediated exon skipping strategy to correct the aberrant splicing. Here, we present data in the wild-type mouse, which demonstrate that intravitreal administration of 2’-OMePS-SSO allows selective alteration of Cep290 splicing in retinal cells, including photoreceptors as shown by successful alteration of Abca4 splicing using the same approach. We show that both SSOs and Cep290 skipped mRNA were detectable for at least 1 month and that intravitreal administration of oligonucleotides did not provoke any serious adverse event. These data suggest that intravitreal injections of SSO should be considered to bypass protein truncation resulting from the c.2991+1655A>G mutation as well as other truncating mutations in genes which like CEP290 or ABCA4 have a mRNA size that exceed cargo capacities of US Food and Drug Administration (FDA-approved adeno-associated virus (AAV-vectors, thus hampering gene augmentation therapy.

  7. Optical Pipelined Multi-bus Interconnection Network Intrinsic Topologies

    Directory of Open Access Journals (Sweden)

    Brian Joseph d'Auriol

    2017-10-01

    Full Text Available Digital all-optical parallel computing is an important research direction and spans conventional devices and convergent nano-optics deployments. Optical bus-based interconnects provide interesting aspects such as relative information communication speed-up or slow-down between optical signals. This aspect is harnessed in the newly proposed All-Optical Linear Array with a Reconfigurable Pipelined Bus System (OLARPBS model. However, the physical realization of such communication interconnects needs to be considered. This paper considers spatial layouts of processing elements along with the optical bus light paths that are necessary to realize the corresponding interconnection requirements. A metric in terms of the degree of required physical constraint is developed to characterize the variety of possible solutions. Simple algorithms that determine spatial layouts are given. It is shown that certain communication interconnection structures have associated intrinsic topologies.

  8. Optimal Locations of Bus Stops Connecting Subways near Urban Intersections

    Directory of Open Access Journals (Sweden)

    Yuan Cui

    2015-01-01

    Full Text Available Unsuitable locations of bus stops which provide feeder transportation connecting subways near urban intersections usually lead to the low efficiency of public transport and level of passenger service. A multiobjective optimization model to distribute such stop locations is proposed to attain the shortest total walk distance of passengers and minimum delay time of cars through intersections and travel time of buses. The Pareto frontier and optimal solutions for the proposed model are given by the distance-based and enumerative methods. The Xizhimen bus stop is selected to implement case studies for verifying the validity of the proposed model. The analysis of sensitivity on possible solutions is also carried out in the case studies. The results show that the proposed model is capable of optimizing the locations of bus stops connecting subways near intersections and helpful to improve the level of passengers service and operational efficiency of public transportation.

  9. American Fuel Cell Bus Project Evaluation. Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-09-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB was developed as part of the Federal Transit Administration's (FTA's) National Fuel Cell Bus Program. Through the non-profit consortia CALSTART, a team led by SunLine Transit Agency and BAE Systems developed a new fuel cell electric bus for demonstration. SunLine added two more AFCBs to its fleet in 2014 and another in 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report summarizes the performance results for the buses through June 2015.

  10. Contagion processes on urban bus networks in Indian cities

    CERN Document Server

    Chatterjee, Atanu; Jagannathan, Krishna

    2015-01-01

    Bus transportation is considered as one of the most convenient and cheapest modes of public transportation in Indian cities. Due to their cost-effectiveness and wide reachability, they help a significant portion of the human population in cities to reach their destinations every day. Although from a transportation point of view they have numerous advantages over other modes of public transportation, they also pose a serious threat of contagious diseases spreading throughout the city. The presence of numerous local spatial constraints makes the process and extent of epidemic spreading extremely difficult to predict. Also, majority of the studies have focused on the contagion processes on scale-free network topologies whereas, spatially-constrained real-world networks such as, bus networks exhibit a wide-spectrum of network topology. Therefore, we aim in this study to understand this complex dynamical process of epidemic outbreak and information diffusion on the bus networks for six different Indian cities usin...

  11. Resolving deconvolution ambiguity in gene alternative splicing

    Directory of Open Access Journals (Sweden)

    Hubbell Earl

    2009-08-01

    Full Text Available Abstract Background For many gene structures it is impossible to resolve intensity data uniquely to establish abundances of splice variants. This was empirically noted by Wang et al. in which it was called a "degeneracy problem". The ambiguity results from an ill-posed problem where additional information is needed in order to obtain an unique answer in splice variant deconvolution. Results In this paper, we analyze the situations under which the problem occurs and perform a rigorous mathematical study which gives necessary and sufficient conditions on how many and what type of constraints are needed to resolve all ambiguity. This analysis is generally applicable to matrix models of splice variants. We explore the proposal that probe sequence information may provide sufficient additional constraints to resolve real-world instances. However, probe behavior cannot be predicted with sufficient accuracy by any existing probe sequence model, and so we present a Bayesian framework for estimating variant abundances by incorporating the prediction uncertainty from the micro-model of probe responsiveness into the macro-model of probe intensities. Conclusion The matrix analysis of constraints provides a tool for detecting real-world instances in which additional constraints may be necessary to resolve splice variants. While purely mathematical constraints can be stated without error, real-world constraints may themselves be poorly resolved. Our Bayesian framework provides a generic solution to the problem of uniquely estimating transcript abundances given additional constraints that themselves may be uncertain, such as regression fit to probe sequence models. We demonstrate the efficacy of it by extensive simulations as well as various biological data.

  12. Stochastic principles governing alternative splicing of RNA

    OpenAIRE

    Jianfei Hu; Eli Boritz; William Wylie; Douek, Daniel C.

    2017-01-01

    Author summary Alternative RNA splicing within eukaryotic cells enables each gene to generate multiple different mature transcripts which further encode proteins with distinct or even opposing functions. The relative frequencies of the transcript isoforms generated by a particular gene are essential to the maintenance of normal cellular physiology; however, the underlying mechanisms and principles that govern these frequencies are unknown. We analyzed the frequency distribution of all transcr...

  13. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    , and regenerative braking for battery charging. It uses a 19.3 kW Ballard PEM fuel cell, will store 12.6 kg of hydrogen at 350 Bar, and includes a 60 kWh battery storage system. The objectives of the project included the following: (a) To advance commercialization of hydrogen-powered transit buses and supporting infrastructure; (b) To provide public outreach and education by showcasing the operation of a 22-foot fuel cell hybrid shuttle bus and Texas first hydrogen fueling infrastructure; and (c) To showcase operation of zero-emissions vehicle for potential transit applications. As mentioned above, the project successfully demonstrated an early vehicle technology, the Ebus plug-in hybrid fuel cell bus, and that success has led to the acquisition of a more advanced vehicle that can take advantage of the same fueling infrastructure. Needed hydrogen station improvements have been identified that will enhance the capabilities of the fueling infrastructure to serve the new bus and to meet the transit agency needs. Over the course of this project, public officials, local government staff, and transit operators were engaged in outreach and education activities that acquainted them with the real world operation of a fuel cell bus and fueling infrastructure. Transit staff members in the Dallas/Ft. Worth region were invited to a workshop in Arlington, Texas at the North Central Texas Council of Governments to participate in a workshop on hydrogen and fuel cells, and to see the fuel cell bus in operation. The bus was trucked to the meeting for this purpose so that participants could see and ride the bus. Austin area transit staff members visited the fueling site in Austin to be briefed on the bus and to participate in a fueling demonstration. This led to further meetings to determine how a fuel cell bus and fueling station could be deployed at Capital Metro Transit. Target urban regions that expressed additional interest during the project in response to the outreach meetings and

  14. ASF/SF2-like maize pre-mRNA splicing factors affect splice site utilization and their transcripts are alternatively spliced.

    Science.gov (United States)

    Gao, Huirong; Gordon-Kamm, William J; Lyznik, L Alexander

    2004-09-15

    Three ASF/SF2-like alternative splicing genes from maize were identified, cloned, and analyzed. Each of these genes (zmSRp30, zmSRp31, and zmSRp32) contains two RNA binding domains, a signature sequence SWQDLKD, and a characteristic serine/ariginine-rich domain. There is a strong structural similarity to the human ASF/SF2 splicing factor and to the Arabidopsis atSRp34/p30 proteins. Similar to ASF/SF2-like genes in other organisms, the maize pre-mRNA messages are alternatively spliced. They are differentially expressed in maize tissues with relatively uniform levels of zmSRp30 and zmSRp31 messages being observed throughout the plant, while zmSRp32 messages preferentially accumulated in the meristematic regions. Overexpression of zmSRp32 in maize cells leads to the enhanced selection of weak 5' intron splice sites during the processing of pre-mRNA molecules. Overexpression of the zmSRp31 or zmSRp32 gene affects regulation of wheat dwarf virus rep gene pre-mRNA splicing, presumably by interacting with the weak 5' splice site, CCGU. Our results suggest that the described genes are functional homologues of the human ASF/SF2 alternative splicing factor and they indicate a diversity of the ASF/SF2-like alternative splicing factors in monocot plant cells.

  15. A hierarchical process for optimizing bus stop distribution

    Directory of Open Access Journals (Sweden)

    Zhengdong Huang

    2014-01-01

    Full Text Available Transit stop spacing is an important indicator in deploying public transit services. This paper presents a hierarchical process for optimizing distribution of bus stops in the context of multi-modal transit development in large cities. Firstly, connection stops are generated manually to connect with other transit facilities. Secondly, key stops are identified based on a coverage model that minimizing total distance, using node centrality value as weight. Thirdly, ordinary bus stops are optimized with coverage model that maximizing potential transit demand. The analytical process is based on a combination of raster and vector data models in Geographical information system, which allows for flexible computing and effective evaluation.

  16. Overview of Multi-DC-Bus Solutions for DC Microgrids

    DEFF Research Database (Denmark)

    Ricchiuto, D.; Mastromauro, R.A.; Liserre, Marco

    2013-01-01

    DC Microgrids have recently received a lot of attention in the last years due to high penetration of renewable energy sources as well as distributed energy storage systems. In the future DC microgrids could be preferable respect to AC microgrids in terms of redundancy since multi-DC-Bus solutions...... could provide a continuative power supply to the loads. An overview of Multi-DC-Bus solutions is presented in this paper. The performances are compared on the basis of possible DC microgrid configurations, redundancy, different DC voltage levels....

  17. Design of temperature monitoring system based on CAN bus

    Science.gov (United States)

    Zhang, Li

    2017-10-01

    The remote temperature monitoring system based on the Controller Area Network (CAN) bus is designed to collect the multi-node remote temperature. By using the STM32F103 as main controller and multiple DS18B20s as temperature sensors, the system achieves a master-slave node data acquisition and transmission based on the CAN bus protocol. And making use of the serial port communication technology to communicate with the host computer, the system achieves the function of remote temperature storage, historical data show and the temperature waveform display.

  18. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Roy, Scott William

    2009-01-01

    of interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs) on exon inclusion levels in human and chimpanzee. For this purpose, we...... compiled and studied comprehensive datasets of predicted ESRs, identified by several computational and experimental approaches, as well as microarray data for changes in alternative splicing levels between human and chimpanzee. Surprisingly, we found no association between changes in predicted ESRs...... and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little...

  19. Accumulation of GC donor splice signals in mammals

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2008-07-01

    Full Text Available Abstract The GT dinucleotide in the first two intron positions is the most conserved element of the U2 donor splice signals. However, in a small fraction of donor sites, GT is replaced by GC. A substantial enrichment of GC in donor sites of alternatively spliced genes has been observed previously in human, nematode and Arabidopsis, suggesting that GC signals are important for regulation of alternative splicing. We used parsimony analysis to reconstruct evolution of donor splice sites and inferred 298 GT > GC conversion events compared to 40 GC > GT conversion events in primate and rodent genomes. Thus, there was substantive accumulation of GC donor splice sites during the evolution of mammals. Accumulation of GC sites might have been driven by selection for alternative splicing. Reviewers This article was reviewed by Jerzy Jurka and Anton Nekrutenko. For the full reviews, please go to the Reviewers' Reports section.

  20. Test and Analysis of Spliced DI-BSCCO HTS Tapes

    Science.gov (United States)

    Fetisov, S. S.; Sotnikov, D. V.; Radchenko, I. P.; Vysotsky, V. S.; Osabe, G.; Kinoshita, K.; Fujikami, J.; Kobayashi, S.; Yamazaki, K.

    For some applications, short unit lengths of HTS wires should be spliced if longer lengths are necessary and short unit lengths of HTS wires should be utilize by applying the splice technology to reduce the total wire cost in the application. The splice technology has been developed for DI-BSCCO Type HT-CA tapes by Sumitomo Electric and spliced tapes were tested in Russian Cable Institute. The test program included: measurements of splice's resistance, critical current anisotropy, thermo cycling tolerance, mechanical properties, overload tests and magnetization measurements. In the paper the results of tests are presented and discussed. The test results demonstrated that splices can be used for cable production if twisting and bending limitations are taken into account.

  1. Splicing modulation therapy in the treatment of genetic diseases

    Directory of Open Access Journals (Sweden)

    Arechavala-Gomeza V

    2014-12-01

    Full Text Available Virginia Arechavala-Gomeza,1 Bernard Khoo,2 Annemieke Aartsma-Rus3 1Neuromuscular Disorders Group, BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain; 2Endocrinology, Division of Medicine, University College London, London, UK; 3Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands All authors contributed equally to this manuscript Abstract: Antisense-mediated splicing modulation is a tool that can be exploited in several ways to provide a potential therapy for rare genetic diseases. This approach is currently being tested in clinical trials for Duchenne muscular dystrophy and spinal muscular atrophy. The present review outlines the versatility of the approach to correct cryptic splicing, modulate alternative splicing, restore the open reading frame, and induce protein knockdown, providing examples of each. Finally, we outline a possible path forward toward the clinical application of this approach for a wide variety of inherited rare diseases. Keywords: splicing, therapy, antisense oligonucleotides, cryptic splicing, alternative splicing

  2. The emerging role of alternative splicing in senescence and aging.

    Science.gov (United States)

    Deschênes, Mathieu; Chabot, Benoit

    2017-10-01

    Deregulation of precursor mRNA splicing is associated with many illnesses and has been linked to age-related chronic diseases. Here we review recent progress documenting how defects in the machinery that performs intron removal and controls splice site selection contribute to cellular senescence and organismal aging. We discuss the functional association linking p53, IGF-1, SIRT1, and ING-1 splice variants with senescence and aging, and review a selection of splicing defects occurring in accelerated aging (progeria), vascular aging, and Alzheimer's disease. Overall, it is becoming increasingly clear that changes in the activity of splicing factors and in the production of key splice variants can impact cellular senescence and the aging phenotype. © 2017 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  3. Splicing Express: a software suite for alternative splicing analysis using next-generation sequencing data

    Directory of Open Access Journals (Sweden)

    Jose E. Kroll

    2015-11-01

    Full Text Available Motivation. Alternative splicing events (ASEs are prevalent in the transcriptome of eukaryotic species and are known to influence many biological phenomena. The identification and quantification of these events are crucial for a better understanding of biological processes. Next-generation DNA sequencing technologies have allowed deep characterization of transcriptomes and made it possible to address these issues. ASEs analysis, however, represents a challenging task especially when many different samples need to be compared. Some popular tools for the analysis of ASEs are known to report thousands of events without annotations and/or graphical representations. A new tool for the identification and visualization of ASEs is here described, which can be used by biologists without a solid bioinformatics background.Results. A software suite named Splicing Express was created to perform ASEs analysis from transcriptome sequencing data derived from next-generation DNA sequencing platforms. Its major goal is to serve the needs of biomedical researchers who do not have bioinformatics skills. Splicing Express performs automatic annotation of transcriptome data (GTF files using gene coordinates available from the UCSC genome browser and allows the analysis of data from all available species. The identification of ASEs is done by a known algorithm previously implemented in another tool named Splooce. As a final result, Splicing Express creates a set of HTML files composed of graphics and tables designed to describe the expression profile of ASEs among all analyzed samples. By using RNA-Seq data from the Illumina Human Body Map and the Rat Body Map, we show that Splicing Express is able to perform all tasks in a straightforward way, identifying well-known specific events.Availability and Implementation.Splicing Express is written in Perl and is suitable to run only in UNIX-like systems. More details can be found at: http://www.bioinformatics-brazil.org/splicingexpress.

  4. Bar-holding prosthetic limb

    Science.gov (United States)

    Vest, Thomas W. (Inventor); Norton, William E. (Inventor); Belcher, Jewell G. (Inventor); Carden, James R. (Inventor)

    1992-01-01

    A prosthetic device for below-the-elbow amputees is disclosed. The device has a removable effector, which is attached to the end of an arm cuff. The effector is comprised of a pair of C-shaped members that are oriented so as to face each other. Working in concert, the C-shaped members are able to hold a bar such as a chainsaw handle. A flat spring is fitted around the C-shaped members to hold them together.

  5. Galaxy Zoo: Observing secular evolution through bars

    Energy Technology Data Exchange (ETDEWEB)

    Cheung, Edmond; Faber, S. M.; Koo, David C. [Department of Astronomy and Astrophysics, 1156 High Street, University of California, Santa Cruz, CA 95064 (United States); Athanassoula, E.; Bosma, A. [Aix Marseille Université, CNRS, LAM (Laboratoire d' Astrophysique de Marseille) UMR 7326, F-13388, Marseille (France); Masters, Karen L.; Nichol, Robert C.; Melvin, Thomas [Institute of Cosmology and Gravitation, University of Portsmouth, Dennis Sciama Building, Portsmouth, PO1 3FX (United Kingdom); Bell, Eric F. [Department of Astronomy, University of Michigan, 500 Church Street, Ann Arbor, MI 48109 (United States); Lintott, Chris [Oxford Astrophysics, Department of Physics, University of Oxford, Denys Wilkinson Building, Keble Road, Oxford OX1 3RH (United Kingdom); Schawinski, Kevin [Institute for Astronomy, Department of Physics, ETH Zurich, Wolfgang-Pauli-Strasse 27, CH-8093 Zurich (Switzerland); Skibba, Ramin A. [Center for Astrophysics and Space Sciences, Department of Physics, 9500 Gilman Drive, University of California, San Diego, CA 92093 (United States); Willett, Kyle W., E-mail: ec2250@gmail.com [School of Physics and Astronomy, University of Minnesota, MN 55455 (United States)

    2013-12-20

    In this paper, we use the Galaxy Zoo 2 data set to study the behavior of bars in disk galaxies as a function of specific star formation rate (SSFR) and bulge prominence. Our sample consists of 13,295 disk galaxies, with an overall (strong) bar fraction of 23.6% ± 0.4%, of which 1154 barred galaxies also have bar length (BL) measurements. These samples are the largest ever used to study the role of bars in galaxy evolution. We find that the likelihood of a galaxy hosting a bar is anticorrelated with SSFR, regardless of stellar mass or bulge prominence. We find that the trends of bar likelihood and BL with bulge prominence are bimodal with SSFR. We interpret these observations using state-of-the-art simulations of bar evolution that include live halos and the effects of gas and star formation. We suggest our observed trends of bar likelihood with SSFR are driven by the gas fraction of the disks, a factor demonstrated to significantly retard both bar formation and evolution in models. We interpret the bimodal relationship between bulge prominence and bar properties as being due to the complicated effects of classical bulges and central mass concentrations on bar evolution and also to the growth of disky pseudobulges by bar evolution. These results represent empirical evidence for secular evolution driven by bars in disk galaxies. This work suggests that bars are not stagnant structures within disk galaxies but are a critical evolutionary driver of their host galaxies in the local universe (z < 1).

  6. Kajian Keamanan Pengoperasian Public Transport Tinjauan Terhadap Kekuatan Rangka Body Bus

    OpenAIRE

    Sudiro, B. M

    2010-01-01

    In order to meet the safety and security of the public transportation, design specification of construction body bus or bus frame structures should have an appropriate structural strength and comply with standard design including the safety factor and selection of material besides static and dynamic testing of the part of body bus. It is also realized that a bus carrying passengers have to give secure to the passengers also driver.The fact that a technology concerning with metal works indus...

  7. Identification of common genetic variation that modulates alternative splicing.

    Directory of Open Access Journals (Sweden)

    Jeremy Hull

    2007-06-01

    Full Text Available Alternative splicing of genes is an efficient means of generating variation in protein function. Several disease states have been associated with rare genetic variants that affect splicing patterns. Conversely, splicing efficiency of some genes is known to vary between individuals without apparent ill effects. What is not clear is whether commonly observed phenotypic variation in splicing patterns, and hence potential variation in protein function, is to a significant extent determined by naturally occurring DNA sequence variation and in particular by single nucleotide polymorphisms (SNPs. In this study, we surveyed the splicing patterns of 250 exons in 22 individuals who had been previously genotyped by the International HapMap Project. We identified 70 simple cassette exon alternative splicing events in our experimental system; for six of these, we detected consistent differences in splicing pattern between individuals, with a highly significant association between splice phenotype and neighbouring SNPs. Remarkably, for five out of six of these events, the strongest correlation was found with the SNP closest to the intron-exon boundary, although the distance between these SNPs and the intron-exon boundary ranged from 2 bp to greater than 1,000 bp. Two of these SNPs were further investigated using a minigene splicing system, and in each case the SNPs were found to exert cis-acting effects on exon splicing efficiency in vitro. The functional consequences of these SNPs could not be predicted using bioinformatic algorithms. Our findings suggest that phenotypic variation in splicing patterns is determined by the presence of SNPs within flanking introns or exons. Effects on splicing may represent an important mechanism by which SNPs influence gene function.

  8. Genomic and Transcriptomic Analysis Reveals Spliced Leader Trans-Splicing in Cryptomonads

    OpenAIRE

    Roy, Scott William

    2017-01-01

    Spliced leader trans-splicing (SLTS) is a poorly understood mechanism that is found in a diversity of eukaryotic lineages. In SLTS, a short RNA sequence is added near the 5′ ends of the transcripts of protein-coding genes by a modified spliceosomal reaction. Available data suggest that SLTS has evolved many times, and might be more likely to evolve in animals. That SLTS might be more likely to evolve in the context of the generally complex transcriptomes characteristic of animals suggests the...

  9. A global analysis of C. elegans trans-splicing

    Science.gov (United States)

    Allen, Mary Ann; Hillier, LaDeana W.; Waterston, Robert H.; Blumenthal, Thomas

    2011-01-01

    Trans-splicing of one of two short leader RNAs, SL1 or SL2, occurs at the 5′ ends of pre-mRNAs of many C. elegans genes. We have exploited RNA-sequencing data from the modENCODE project to analyze the transcriptome of C. elegans for patterns of trans-splicing. Transcripts of ∼70% of genes are trans-spliced, similar to earlier estimates based on analysis of far fewer genes. The mRNAs of most trans-spliced genes are spliced to either SL1 or SL2, but most genes are not trans-spliced to both, indicating that SL1 and SL2 trans-splicing use different underlying mechanisms. SL2 trans-splicing occurs in order to separate the products of genes in operons genome wide. Shorter intercistronic distance is associated with greater use of SL2. Finally, increased use of SL1 trans-splicing to downstream operon genes can indicate the presence of an extra promoter in the intercistronic region, creating what has been termed a “hybrid” operon. Within hybrid operons the presence of the two promoters results in the use of the two SL classes: Transcription that originates at the promoter upstream of another gene creates a polycistronic pre-mRNA that receives SL2, whereas transcription that originates at the internal promoter creates transcripts that receive SL1. Overall, our data demonstrate that >17% of all C. elegans genes are in operons. PMID:21177958

  10. Regulation of alternative splicing in human obesity loci.

    Science.gov (United States)

    Kaminska, Dorota; Käkelä, Pirjo; Nikkola, Elina; Venesmaa, Sari; Ilves, Imre; Herzig, Karl-Heinz; Kolehmainen, Marjukka; Karhunen, Leila; Kuusisto, Johanna; Gylling, Helena; Pajukanta, Päivi; Laakso, Markku; Pihlajamäki, Jussi

    2016-10-01

    Multiple obesity susceptibility loci have been identified by genome-wide association studies, yet the mechanisms by which these loci influence obesity remain unclear. Alternative splicing could contribute to obesity by regulating the transcriptomic and proteomic diversity of genes in these loci. Based on a database search, 72 of the 136 genes at the 13 obesity loci encoded multiple protein isoforms. Thus, alternative splicing of these genes in adipose tissue samples was analyzed from the Metabolic Syndrome in Men population-based study and from two weight loss intervention studies (surgical and very low calorie diet). Alternative splicing was confirmed in 11 genes with PCR capillary electrophoresis in human subcutaneous adipose tissue. Interestingly, differential splicing of TRA2B, BAG6, and MSH5 was observed between lean individuals with normoglycemia and overweight individuals with type 2 diabetes. Of these genes, we detected fat depot-dependent splicing of TRA2B and BAG6 and weight loss-induced regulation of MSH5 splicing in the intervention studies. Finally, body mass index was a major determinant of TRA2B, BAG6, and MSH5 splicing in the combined data. This study provides evidence for alternative splicing in obesity loci, suggesting that alternative splicing at least in part mediates the obesity-associated risk in these loci. © 2016 The Obesity Society.

  11. Genome-wide analysis of alternative splicing in Chlamydomonas reinhardtii

    Directory of Open Access Journals (Sweden)

    Thomas Julie

    2010-02-01

    Full Text Available Abstract Background Genome-wide computational analysis of alternative splicing (AS in several flowering plants has revealed that pre-mRNAs from about 30% of genes undergo AS. Chlamydomonas, a simple unicellular green alga, is part of the lineage that includes land plants. However, it diverged from land plants about one billion years ago. Hence, it serves as a good model system to study alternative splicing in early photosynthetic eukaryotes, to obtain insights into the evolution of this process in plants, and to compare splicing in simple unicellular photosynthetic and non-photosynthetic eukaryotes. We performed a global analysis of alternative splicing in Chlamydomonas reinhardtii using its recently completed genome sequence and all available ESTs and cDNAs. Results Our analysis of AS using BLAT and a modified version of the Sircah tool revealed AS of 498 transcriptional units with 611 events, representing about 3% of the total number of genes. As in land plants, intron retention is the most prevalent form of AS. Retained introns and skipped exons tend to be shorter than their counterparts in constitutively spliced genes. The splice site signals in all types of AS events are weaker than those in constitutively spliced genes. Furthermore, in alternatively spliced genes, the prevalent splice form has a stronger splice site signal than the non-prevalent form. Analysis of constitutively spliced introns revealed an over-abundance of motifs with simple repetitive elements in comparison to introns involved in intron retention. In almost all cases, AS results in a truncated ORF, leading to a coding sequence that is around 50% shorter than the prevalent splice form. Using RT-PCR we verified AS of two genes and show that they produce more isoforms than indicated by EST data. All cDNA/EST alignments and splice graphs are provided in a website at http://combi.cs.colostate.edu/as/chlamy. Conclusions The extent of AS in Chlamydomonas that we observed is much

  12. Splice Site Mutations in the ATP7A Gene

    DEFF Research Database (Denmark)

    Skjørringe, Tina; Tümer, Zeynep; Møller, Lisbeth Birk

    2011-01-01

    Menkes disease (MD) is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12...... mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation...

  13. Characterizing HIV-1 Splicing by Using Next-Generation Sequencing.

    Science.gov (United States)

    Emery, Ann; Zhou, Shuntai; Pollom, Elizabeth; Swanstrom, Ronald

    2017-03-15

    Full-length human immunodeficiency virus type 1 (HIV-1) RNA serves as the genome or as an mRNA, or this RNA undergoes splicing using four donors and 10 acceptors to create over 50 physiologically relevant transcripts in two size classes (1.8 kb and 4 kb). We developed an assay using Primer ID-tagged deep sequencing to quantify HIV-1 splicing. Using the lab strain NL4-3, we found that A5 (env/nef) is the most commonly used acceptor (about 50%) and A3 (tat) the least used (about 3%). Two small exons are made when a splice to acceptor A1 or A2 is followed by activation of donor D2 or D3, and the high-level use of D2 and D3 dramatically reduces the amount of vif and vpr transcripts. We observed distinct patterns of temperature sensitivity of splicing to acceptors A1 and A2. In addition, disruption of a conserved structure proximal to A1 caused a 10-fold reduction in all transcripts that utilized A1. Analysis of a panel of subtype B transmitted/founder viruses showed that splicing patterns are conserved, but with surprising variability of usage. A subtype C isolate was similar, while a simian immunodeficiency virus (SIV) isolate showed significant differences. We also observed transsplicing from a downstream donor on one transcript to an upstream acceptor on a different transcript, which we detected in 0.3% of 1.8-kb RNA reads. There were several examples of splicing suppression when the env intron was retained in the 4-kb size class. These results demonstrate the utility of this assay and identify new examples of HIV-1 splicing regulation. IMPORTANCE During HIV-1 replication, over 50 conserved spliced RNA variants are generated. The splicing assay described here uses new developments in deep-sequencing technology combined with Primer ID-tagged cDNA primers to efficiently quantify HIV-1 splicing at a depth that allows even low-frequency splice variants to be monitored. We have used this assay to examine several features of HIV-1 splicing and to identify new examples of

  14. Adenosine to Inosine editing frequency controlled by splicing efficiency.

    Science.gov (United States)

    Licht, Konstantin; Kapoor, Utkarsh; Mayrhofer, Elisa; Jantsch, Michael F

    2016-07-27

    Alternative splicing and adenosine to inosine (A to I) RNA-editing are major factors leading to co- and post-transcriptional modification of genetic information. Both, A to I editing and splicing occur in the nucleus. As editing sites are frequently defined by exon-intron basepairing, mRNA splicing efficiency should affect editing levels. Moreover, splicing rates affect nuclear retention and will therefore also influence the exposure of pre-mRNAs to the editing-competent nuclear environment. Here, we systematically test the influence of splice rates on RNA-editing using reporter genes but also endogenous substrates. We demonstrate for the first time that the extent of editing is controlled by splicing kinetics when editing is guided by intronic elements. In contrast, editing sites that are exclusively defined by exonic structures are almost unaffected by the splicing efficiency of nearby introns. In addition, we show that editing levels in pre- and mature mRNAs do not match. This phenomenon can in part be explained by the editing state of an RNA influencing its splicing rate but also by the binding of the editing enzyme ADAR that interferes with splicing. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  15. 49 CFR 571.220 - Standard No. 220; School bus rollover protection.

    Science.gov (United States)

    2010-10-01

    ... result from failure of the school bus body structure to withstand forces encountered in rollover crashes... 49 Transportation 6 2010-10-01 2010-10-01 false Standard No. 220; School bus rollover protection... STANDARDS Federal Motor Vehicle Safety Standards § 571.220 Standard No. 220; School bus rollover protection...

  16. Bus Operations for Pupil Transportation. "Chrome-Yellow and Red Lights."

    Science.gov (United States)

    Rannels, Morris W.

    This paper is based on a committee report of the last National School Bus Conference on "Minimum Standards for School Buses." Standards for school bus operations are included for (1) administrators, (2) supervisors, (3) school bus drivers, (4) student passengers, and (5) maintenance and service personnel. The report deals also with procedures for…

  17. DETERMINATION OF THE DELAY DEPARTURE TIME OF THE BUS FROM STOPPING POINT INTO THE CAR FLOW

    Directory of Open Access Journals (Sweden)

    P. Gorbachov

    2014-10-01

    Full Text Available An analytical model for estimation of the bus departure delax from the bus stopping point after passenger loading is presented. The model allows to take into account the traffic density on a particular road section of the transport network, as well as the bus parameters.

  18. Air flow through a non-airconditioned bus with open windows

    Indian Academy of Sciences (India)

    Smoke and tuft visualizations were also performed on an urban bus at 40 km/h. Numerical simulations were performed at the actual Reynolds number. Even though there were Reynolds number differences, the broad features were similar. Air enters the bus from the rear windows, moves to the front (relative to the bus) and ...

  19. Survey on the implementation and reliability of CubeSat electrical bus interfaces

    NARCIS (Netherlands)

    Bouwmeester, J.; Langer, M; Gill, E.K.A.

    2016-01-01

    This paper provides results and conclusions on a survey on the implementation and reliability aspects of CubeSat bus interfaces, with an emphasis on the data bus and power distribution. It provides recommendations for a future CubeSat bus standard. The survey is based on a literature study and a

  20. School Bus Safety: What Can Our Schools Do to Protect Our Children?

    Science.gov (United States)

    Dargan, Thomas J.; Silverstone, Adam H.

    2014-01-01

    School districts and school bus contractors are entrusted with the most important of all road users--our nation's children. In the wake of recent newsworthy accidents and attention grabbing headlines regarding unfit bus drivers, claims premised upon school bus accidents have become increasingly tangential and, in turn, personal injury attorneys…

  1. Computer-Based Video Instruction to Teach Students with Intellectual Disabilities to Use Public Bus Transportation

    Science.gov (United States)

    Mechling, Linda; O'Brien, Eileen

    2010-01-01

    This study investigated the effectiveness of computer-based video instruction (CBVI) to teach three young adults with moderate intellectual disabilities to push a "request to stop bus signal" and exit a city bus in response to target landmarks. A multiple probe design across three students and one bus route was used to evaluate effectiveness of…

  2. Trajectories of Cigarette Smoking among Commercial Bus Drivers ...

    African Journals Online (AJOL)

    Trajectories of Cigarette Smoking among Commercial Bus Drivers and University Students in Nigeria. ... Thus, this article gathered comprehensive primary qualitative, quantitative and secondary data on smoking behaviors of two distinct groups in Nigeria for reliable comprehension of smoking trajectories in Nigeria with ...

  3. A Quantitative Economics Of Seasonality In Bus Patronage In Nigeria

    African Journals Online (AJOL)

    The study thus set out to empirically investigate the myth or reality of the thesis that trip generation and trip attraction can be significantly season- determined. Collecting time series data over a ... Apposite recommendations were then made, sequel to highlighting of policy implications of findings. Key words: seasonality, bus ...

  4. Eliminating the empty bus syndrome by understanding dynamic travel behaviour

    CSIR Research Space (South Africa)

    Mokonyama, Mathetha T

    2012-11-01

    Full Text Available This chapter interrogates some empirical data to examine some of the contributory causes of EBS in South Africa, and uses the City of Johannesburg as a case study. The daily travel patterns of car users are analysed within the context of how bus...

  5. A Novel Time Synchronization Method for Dynamic Reconfigurable Bus

    Directory of Open Access Journals (Sweden)

    Zhang Weigong

    2016-01-01

    Full Text Available UM-BUS is a novel dynamically reconfigurable high-speed serial bus for embedded systems. It can achieve fault tolerance by detecting the channel status in real time and reconfigure dynamically at run-time. The bus supports direct interconnections between up to eight master nodes and multiple slave nodes. In order to solve the time synchronization problem among master nodes, this paper proposes a novel time synchronization method, which can meet the requirement of time precision in UM-BUS. In this proposed method, time is firstly broadcasted through time broadcast packets. Then, the transmission delay and time deviations via three handshakes during link self-checking and channel detection can be worked out referring to the IEEE 1588 protocol. Thereby, each node calibrates its own time according to the broadcasted time. The proposed method has been proved to meet the requirement of real-time time synchronization. The experimental results show that the synchronous precision can achieve a bias less than 20 ns.

  6. Heavy Duty Diesel Truck and Bus Hybrid Powertrain Study

    Science.gov (United States)

    2012-03-01

    4.1.4 Kenworth Truck Company (PACCAR Inc.) ................................................................... 72 4.1.5 Mack Trucks Inc. ( Volvo ...76 4.1.7 Nova Bus ( Volvo Group...74 4.1.5 Mack Trucks Inc. ( Volvo Group) Mack Trucks does not yet have any diesel-electric hybrid truck models in series production, although its

  7. Cascaded Amplifying Quantum Optical Taps: A Robust Noiseless Optical Bus

    Science.gov (United States)

    Bencheikh, K.; Simonneau, C.; Levenson, J. A.

    1997-01-01

    Two identical amplifying quantum optical taps, based on noiseless optical parametric amplification and twin beam quantum correlation, have been implemented in a series configuration and experimentally investigated. The result is an optical bus which we have shown to be robust with respect to downstream losses.

  8. Measuring Bus Drivers' Occupational Stress Under Changing Working Conditions

    NARCIS (Netherlands)

    Hlotova, Y.; Cats, O.; Meijer, S.A.

    2014-01-01

    Stress is an immense problem in modern society; approximately half of all occupational illnesses are directly or indirectly related to stress. The work of a bus driver is typically associated with high stress levels that negatively influence individual well-being as well as workforce management. The

  9. Distributed cooperative synchronization strategy for multi-bus microgrids

    DEFF Research Database (Denmark)

    Sun, Yao; Zhong, Chaolu; Hou, Xiaochao

    2017-01-01

    synchronization methods based on centralized communication are very costly and not suitable for multi-bus microgrids that have a large number of distributed generators (DGs). To address this concern, this study presents an active synchronization control strategy based on distributed cooperation technology...

  10. Implementation of a Walking School Bus: Lessons Learned

    Science.gov (United States)

    Kong, Alberta S.; Sussman, Andrew L.; Negrete, Sylvia; Patterson, Nissa; Mittleman, Rachel; Hough, Richard

    2009-01-01

    Background: Obesity is rapidly becoming the most common chronic medical condition affecting children. To address this crisis, an Albuquerque, New Mexico, elementary school partnered with University of New Mexico researchers and conducted a Walking School Bus (WSB). The purpose of this article is to examine the feasibility of implementing a WSB.…

  11. Bussikontsern Go Bus ihaldab Harjumaa turgu / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2005-01-01

    AS-i Tarbus ja kaheksa bussiettevõtte ühinemisel moodustati AS Go Bus, ettevõte plaanib veel mitme bussifirma liitmist. Atko Grupi juhatuse esimehe Margo Tomingase, AS Sebe nõukogu esimehe Hugo Osula arvamus. Lisa: Konkurentsiamet hoiab silma peal

  12. Fairness in optimizing bus-crew scheduling process.

    Science.gov (United States)

    Ma, Jihui; Song, Cuiying; Ceder, Avishai Avi; Liu, Tao; Guan, Wei

    2017-01-01

    This work proposes a model considering fairness in the problem of crew scheduling for bus drivers (CSP-BD) using a hybrid ant-colony optimization (HACO) algorithm to solve it. The main contributions of this work are the following: (a) a valid approach for cases with a special cost structure and constraints considering the fairness of working time and idle time; (b) an improved algorithm incorporating Gamma heuristic function and selecting rules. The relationships of each cost are examined with ten bus lines collected from the Beijing Public Transport Holdings (Group) Co., Ltd., one of the largest bus transit companies in the world. It shows that unfair cost is indirectly related to common cost, fixed cost and extra cost and also the unfair cost approaches to common and fixed cost when its coefficient is twice of common cost coefficient. Furthermore, the longest time for the tested bus line with 1108 pieces, 74 blocks is less than 30 minutes. The results indicate that the HACO-based algorithm can be a feasible and efficient optimization technique for CSP-BD, especially with large scale problems.

  13. High-Tech School Bus Teaches Students on the Road

    Science.gov (United States)

    Katims, Lauren

    2011-01-01

    Last year, kindergarten through high school students in the rural Hector, Arkansas, School District barely had the technology resources that keep kids interested in math and science. This year, they potentially have the most advanced resources in the country--before they even step into the classroom. One school bus in Arkansas' Pope County has…

  14. Low back trouble among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1989-01-01

    herniation was 137 compared to all Danish men. These differences were statistically significant. Of psychosocial factors only "the feeling of being mentally unbalanced" was found to be statistically significantly related to subsequent hospital discharge with a LBT-diagnosis. Long seniority as bus driver...

  15. Aerodynamic study of state transport bus using computational fluid dynamics

    Science.gov (United States)

    Kanekar, Siddhesh; Thakre, Prashant; Rajkumar, E.

    2017-11-01

    The main purpose of this study was to develop the aerodynamic study of a Maharashtra state road transport bus. The rising fuel price and strict government regulations makes the road transport uneconomical now days. With the objective of increasing fuel efficiency and reducing the emission of harmful exhaust gases. It has been proven experimentally that vehicle consumes almost 40% of the available useful engine power to overcome the drag resistance. This provides us a huge scope to study the influence of aerodynamic drag. The initial of the project was to identify the drag coefficient of the existing ordinary type model called “Parivartan” from ANSYS fluent. After preliminary analysis of the existing model corresponding changes are made in such a way that their implementation should be possible at workshop level. The simulation of the air flow over the bus was performed in two steps: design on SolidWorks CAD and ANSYS (FLUENT) is used as a virtual analysis tool to estimate the drag coefficient of the bus. We have used the turbulence models k-ε Realizable having a better approximation of the actual result. Around 28% improvement in the drag coefficient is achieved by CFD driven changes in the bus design. Coefficient of drag is improved by 28% and fuel efficiency increased by 20% by CFD driven changes.

  16. Scheduling with Bus Access Optimization for Distributed Embedded Systems

    DEFF Research Database (Denmark)

    Eles, Petru; Doboli, Alex; Pop, Paul

    2000-01-01

    In this paper, we concentrate on aspects related to the synthesis of distributed embedded systems consisting of programmable processors and application-specific hardware components. The approach is based on an abstract graph representation that captures, at process level, both dataflow and the flow......, generates an efficient bus access scheme as well as the schedule tables for activation of processes and communications....

  17. Training Needs Analysis for Bus Depot Managers at GSRTC

    Science.gov (United States)

    Shah, Hardik; Gopal, Raj

    2012-01-01

    Purpose: With rapid industrialization and population growth in the urban and rural areas in India, the demand for public transport has risen sharply. In the fast changing scenario in the public transit sector, the role of a bus depot manager (DM) has also undergone substantial transformation. This paper aims to identify and gauge the…

  18. The Taking Ways of an Old School Bus

    Science.gov (United States)

    Kelley, Joan Thompson; Watkins, J. Foster

    1969-01-01

    The conversion of an "old school bus" in the late 1960s into a mobile library is discussed. The conversion was an element of educational service developed cooperatively between a regional educational laboratory and a rural school system. The project endeavored to improve the early experiences of future students, many of whom would have…

  19. ADlll:S ABABA BUS TERMINAL DESIGN COMPETITION.

    African Journals Online (AJOL)

    Ethiopian Association of Engineers and Architects. Today at the opening of the First National. Conference of the ... of the Architectural Competition for the New Bus. Terminal of Addis Ababa. Unfortunately, due to another ... A competition enables the client, the future owner of the project, to choose from various solutions.

  20. Real life testing of a Hybrid PEM Fuel Cell Bus

    Science.gov (United States)

    Folkesson, Anders; Andersson, Christian; Alvfors, Per; Alaküla, Mats; Overgaard, Lars

    Fuel cells produce low quantities of local emissions, if any, and are therefore one of the most promising alternatives to internal combustion engines as the main power source in future vehicles. It is likely that urban buses will be among the first commercial applications for fuel cells in vehicles. This is due to the fact that urban buses are highly visible for the public, they contribute significantly to air pollution in urban areas, they have small limitations in weight and volume and fuelling is handled via a centralised infrastructure. Results and experiences from real life measurements of energy flows in a Scania Hybrid PEM Fuel Cell Concept Bus are presented in this paper. The tests consist of measurements during several standard duty cycles. The efficiency of the fuel cell system and of the complete vehicle are presented and discussed. The net efficiency of the fuel cell system was approximately 40% and the fuel consumption of the concept bus is between 42 and 48% lower compared to a standard Scania bus. Energy recovery by regenerative braking saves up 28% energy. Bus subsystems such as the pneumatic system for door opening, suspension and brakes, the hydraulic power steering, the 24 V grid, the water pump and the cooling fans consume approximately 7% of the energy in the fuel input or 17% of the net power output from the fuel cell system. The bus was built by a number of companies in a project partly financed by the European Commission's Joule programme. The comprehensive testing is partly financed by the Swedish programme "Den Gröna Bilen" (The Green Car). A 50 kW el fuel cell system is the power source and a high voltage battery pack works as an energy buffer and power booster. The fuel, compressed hydrogen, is stored in two high-pressure stainless steel vessels mounted on the roof of the bus. The bus has a series hybrid electric driveline with wheel hub motors with a maximum power of 100 kW. Hybrid Fuel Cell Buses have a big potential, but there are

  1. GeoBus: sharing science research with schools

    Science.gov (United States)

    Roper, Kathryn; Robinson, Ruth; Moorhouse, Ben

    2016-04-01

    GeoBus (www.geobus.org.uk) is an educational outreach project that was developed in 2012 by the Department of Earth and Environmental Sciences at the University of St Andrews, and it is currently sponsored by industry, NERC, The Crown Estate, and the Scottish Government. The aims of GeoBus are to support the teaching of Earth Science in secondary (middle and high) schools by providing teaching support to schools that have little or no experience in teaching this subject. This is, in part, done through the sharing of new science research outcomes and the experiences of young researchers with school pupils to provide a bridge between industry, higher education institutions, research councils and schools. Since its launch, over 40,000 pupils will have been involved in experiential Earth science learning activities in 190 different schools (over 400 separate visits) across the length and breadth of Scotland: many of these schools are in remote and disadvantaged regions. A new GeoBus project is under development within the Department of Earth Sciences at UCL in London. A key aim of GeoBus is to incorporate new research into our workshops with the main challenge being the development of appropriate resources that incorporate the key learning aims and requirements of the science and geography curricula. GeoBus works closely with researchers, teachers and educational practitioners to tailor the research outcomes to the curricula as much as possible. Over the past four years, GeoBus has developed 17 workshops, 5 challenge events and extensive field trips and each of these activities are trialled and evaluated within the university, and adjustments are made before the activities are delivered in schools. Activities are continually reviewed and further developments are made in response to both teacher and pupil feedback. This critical reflection of the project's success and impact is important to insure a positive and significant contribution to the science learning in

  2. Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process

    Directory of Open Access Journals (Sweden)

    Katja Meyer

    2015-07-01

    Full Text Available Alternative pre-messenger RNA splicing in higher plants emerges as an important layer of regulation upon exposure to exogenous and endogenous cues. Accordingly, mutants defective in RNA-binding proteins predicted to function in the splicing process show severe phenotypic alterations. Among those are developmental defects, impaired responses to pathogen threat or abiotic stress factors, and misregulation of the circadian timing system. A suite of splicing factors has been identified in the model plant Arabidopsis thaliana. Here we summarize recent insights on how defects in these splicing factors impair plant performance.

  3. THE PREVALENCE OF MUSCULOSKELETAL DISORDERS AMONG BUS DRIVERS IN TRICITY

    Directory of Open Access Journals (Sweden)

    Lalit

    2015-10-01

    Full Text Available Background: Musculoskeletal disorders are widespread in many countries around the world. It has been reported that about 58 percent of the world's population over the age of 10 years spent one third of their life span at work. The population at a high risk include nursing facilities, transportation, mining, food processing, leather tanning, heavy and light manufacturing. Transport workers have been found to be at high risk of developing work related musculoskeletal disorders (WRMSDs. There has been literature evidence regarding the prevalence of musculoskeletal disorders in bus drivers of various cities of different countries. But no study has been done so far in Tricity (Chandigarh, Panchkula and Mohali for the same. The purpose of this study is to investigate the prevalence and characteristics of work related musculoskeletal disorders (WRMSDs among bus drivers of Tricity. Methods: 300 bus drivers were included in the study according to the inclusion and exclusion criteria. The standardized Nordic questionnaire for musculoskeletal disorder and a self administered questionnaire were filled by therapist after the personal interview of each driver. Results: Unpaired t test was used to measure the difference in variable of two groups and Karl Pearson’s correlation coefficient was used to determine the correlation between two entities. In the present study, the subjects were in the age group of 25 to 50 years. Out of the total sample of 300 male bus drivers in Tricity, 159 reported that they had WRMSDs. The prevalence of WRMSDs among bus drivers in Tricity was 53%. In present study, the prevalence of low back pain was highest among the bus drivers that are 30.3%, then neck pain 17.3%, knee pain 14.7%, shoulder 6.3%, ankle and feet 5.7%, upper back 4%, hip and thigh 4%, elbow 1.3% and wrist and hand 1.3%. Thus low back pain, neck pain and knee pain are the most prevalent WRMSDs amongst bus drivers. Conclusions: Work-related biomechanical

  4. Avoiding DWI among Bar-room Drinkers: Strategies and Predictors

    Directory of Open Access Journals (Sweden)

    Barry D. Caudill

    2010-01-01

    Full Text Available We examined the prevalence and predictors of 11strategies to avoid driving when feeling intoxicated among 561 bar-room patrons in two medium-sized Maryland communities. Logistic regression analyses identified demographic, behavioral, and attitudinal predictors of avoidance strategies and interactions among predictors. Overall, 89% reported one or more DWI avoidance actions in the past year, and 38% reported driving intoxicated during that time. Average frequencies of avoidance behavior and intoxicated driving increased significantly as drinking level increased. However, the higher the drinking level, the smaller the ratio of avoidance actions to DWI experiences, highlighting the vulnerability of heavy drinkers who had driven intoxicated. Using a sober driver or one who allegedly drank less than the respondent were the most popular and frequent strategies, but paying for a cab, walking, and using a bus or free cab were relatively unpopular. Higher drinking levels predicted significantly higher odds of using avoidance approaches, as did intoxicated driving. Confidence in driving safely when intoxicated was positively related to drinking level and intoxicated driving, but it tended to predict lower odds of avoidance actions. Similarly, marital status, age, gender, and location influenced the odds of avoidance behaviors. Interventions should be strategically tailored to exploit or counter drinker predilections among avoidance options.

  5. Alternate trans splicing in Trypanosoma equiperdum: implications for splice site selection.

    Science.gov (United States)

    Layden, R E; Eisen, H

    1988-03-01

    We examined the structures of the 5' ends of mRNAs encoding variant surface glycoprotein 78 (VSG-78) and VSG-1(78) in Trypanosoma equiperdum. Several mRNA species were found for each gene, and all contained the 35-base miniexon (or spliced leader) sequence attached at different positions on their 5' ends. Thus, the generation of multiple messages for each VSG occurred by attachment of the miniexon at one of several 3' splice acceptor sites. The frequency with which individual splice sites were used varied from less than 1 to 95% of the RNA produced from a particular gene. We propose that the miniexon RNA and RNA from the VSG genes may interact via base pairing and that this in part specifies the use of particular acceptor sites. Sequences complementary to the miniexon primary transcript, termed the "med-comp site," were found in both genes and in several published sequences. Splice sites were most often used if they were the first site 3' of the med-comp site and contained a high pyrimidine content in the bases preceding the AG acceptor signal.

  6. Trans-splicing and operons in C. elegans.

    Science.gov (United States)

    Blumenthal, Thomas

    2012-11-20

    About 70% of C. elegans mRNAs are trans-spliced to one of two 22 nucleotide spliced leaders. SL1 is used to trim off the 5' ends of pre-mRNAs and replace them with the SL1 sequence. This processing event is very closely related to cis-splicing, or intron removal. The SL1 sequence is donated by a 100 nt small nuclear ribonucleoprotein particle (snRNP), the SL1 snRNP. This snRNP is structurally and functionally similar to the U snRNAs (U1, U2, U4, U5 and U6) that play key roles in intron removal and trans-splicing, except that the SL1 snRNP is consumed in the process. More than half of C. elegans pre-mRNAs are subject to SL1 trans-splicing, whereas ~30% are not trans-spliced. The remaining genes are trans-spliced by SL2, which is donated by a similar snRNP, the SL2 snRNP. SL2 recipients are all downstream genes in closely spaced gene clusters similar to bacterial operons. They are transcribed from a promoter at the 5' end of the cluster of between 2 and 8 genes. This transcription makes a polycistronic pre-mRNA that is co-transcriptionally processed by cleavage and polyadenylation at the 3' end of each gene, and this event is closely coupled to the SL2 trans-splicing event that occurs only ~100 nt further downstream. SL2 trans-splicing requires a sequence between the genes, the Ur element, that likely base pairs with the 5' splice site on the SL2 snRNP, in a manner analogous to the interaction between the 5' splice site in cis-splicing with the U1 snRNP. The key difference is that in trans-splicing, the snRNP contains the 5' splice site, whereas in cis-splicing the pre-mRNA does. Some operons, termed "hybrid operons", contain an additional promoter between two genes that can express the downstream gene or genes with a developmental profile that is different from that of the entire operon. The operons contain primarily genes required for rapid growth, including genes whose products are needed for mitochondrial function and the basic machinery of gene expression

  7. Bus Stops Location and Bus Route Planning Using Mean Shift Clustering and Ant Colony in West Jakarta

    Science.gov (United States)

    Supangat, Kenny; Eko Soelistio, Yustinus

    2017-03-01

    Traffic Jam has been a daily problem for people in Jakarta which is one of the busiest city in Indonesia up until now. Even though the official government has tried to reduce the impact of traffic issues by developing a new public transportation which takes up a lot of resources and time, it failed to diminish the problem. The actual concern to this problem actually lies in how people move between places in Jakarta where they always using their own vehicle like cars, and motorcycles that fill most of the street in Jakarta. Among much other public transportations that roams the street of Jakarta, Buses is believed to be an efficient transportation that can move many people at once. However, the location of the bus stop is now have moved to the middle of the main road, and its too far for the nearby residence to access to it. This paper proposes an optimal location of optimal bus stops in West Jakarta that is experimentally proven to have a maximal distance of 350 m. The optimal location is estimated by means of mean shift clustering method while the optimal routes are calculated using Ant Colony algorithm. The bus stops locations rate of error is 0.07% with overall route area of 32 km. Based on our experiments, we believe our proposed bus stop plan can be an interesting alternative to reduce traffic congestion in West Jakarta.

  8. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-03-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This report provides the early data results and implementation experience of the AT fuel cell bus since it was placed in service.

  9. Functions for fission yeast splicing factors SpSlu7 and SpPrp18 in alternative splice-site choice and stress-specific regulated splicing.

    Directory of Open Access Journals (Sweden)

    Geetha Melangath

    Full Text Available Budding yeast spliceosomal factors ScSlu7 and ScPrp18 interact and mediate intron 3'ss choice during second step pre-mRNA splicing. The fission yeast genome with abundant multi-intronic transcripts, degenerate splice signals and SR proteins is an apt unicellular fungal model to deduce roles for core spliceosomal factors in alternative splice-site choice, intron retention and to study the cellular implications of regulated splicing. From our custom microarray data we deduce a stringent reproducible subset of S. pombe alternative events. We examined the role of factors SpSlu7 or SpPrp18 for these splice events and investigated the relationship to growth phase and stress. Wild-type log and stationary phase cells showed ats1+ exon 3 skipped and intron 3 retained transcripts. Interestingly the non-consensus 5'ss in ats1+ intron 3 caused SpSlu7 and SpPrp18 dependent intron retention. We validated the use of an alternative 5'ss in dtd1+ intron 1 and of an upstream alternative 3'ss in DUF3074 intron 1. The dtd1+ intron 1 non-canonical 5'ss yielded an alternative mRNA whose levels increased in stationary phase. Utilization of dtd1+ intron 1 sub-optimal 5' ss required functional SpPrp18 and SpSlu7 while compromise in SpSlu7 function alone hampered the selection of the DUF3074 intron 1 non canonical 3'ss. We analysed the relative abundance of these splice isoforms during mild thermal, oxidative and heavy metal stress and found stress-specific splice patterns for ats1+ and DUF3074 intron 1 some of which were SpSlu7 and SpPrp18 dependent. By studying ats1+ splice isoforms during compromised transcription elongation rates in wild-type, spslu7-2 and spprp18-5 mutant cells we found dynamic and intron context-specific effects in splice-site choice. Our work thus shows the combinatorial effects of splice site strength, core splicing factor functions and transcription elongation kinetics to dictate alternative splice patterns which in turn serve as an additional

  10. Jackson Bar Training Structure Study

    Science.gov (United States)

    2015-05-01

    comparison of the one-dimensional bridge hydraulic routines from: HEC - RAS , HEC -2, and WSPRO. Davis, CA: U.S. Army Corps of Engineers, Hydrologic Engineering...ER D C/ CH L TR -1 5- 4 Jackson Bar Training Structure Study Co as ta l a nd H yd ra ul ic s La bo ra to ry Jeremy A. Sharp and...Leroy Gage), a previously constructed HEC -2 model, and a previously constructed WES physical model from 1987. Three alternatives were modeled in an

  11. Approaches to link RNA secondary structures with splicing regulation

    DEFF Research Database (Denmark)

    Plass, Mireya; Eyras, Eduardo

    2014-01-01

    In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by facilitat...

  12. Revealing the Determinants of Widespread Alternative Splicing Perturbation in Cancer

    Directory of Open Access Journals (Sweden)

    Yongsheng Li

    2017-10-01

    Full Text Available It is increasingly appreciated that alternative splicing plays a key role in generating functional specificity and diversity in cancer. However, the mechanisms by which cancer mutations perturb splicing remain unknown. Here, we developed a network-based strategy, DrAS-Net, to investigate more than 2.5 million variants across cancer types and link somatic mutations with cancer-specific splicing events. We identified more than 40,000 driver variant candidates and their 80,000 putative splicing targets deregulated in 33 cancer types and inferred their functional impact. Strikingly, tumors with splicing perturbations show reduced expression of immune system-related genes and increased expression of cell proliferation markers. Tumors harboring different mutations in the same gene often exhibit distinct splicing perturbations. Further stratification of 10,000 patients based on their mutation-splicing relationships identifies subtypes with distinct clinical features, including survival rates. Our work reveals how single-nucleotide changes can alter the repertoires of splicing isoforms, providing insights into oncogenic mechanisms for precision medicine.

  13. Alternative Splicing of STAT3 Is Affected by RNA Editing.

    Science.gov (United States)

    Goldberg, Lior; Abutbul-Amitai, Mor; Paret, Gideon; Nevo-Caspi, Yael

    2017-05-01

    A-to-I RNA editing, carried out by adenosine deaminase acting on RNA (ADAR) enzymes, is an epigenetic phenomenon of posttranscriptional modifications on pre-mRNA. RNA editing in intronic sequences may influence alternative splicing of flanking exons. We have previously shown that conditions that induce editing result in elevated expression of signal transducer and activator of transcription 3 (STAT3), preferentially the alternatively-spliced STAT3β isoform. Mechanisms regulating alternative splicing of STAT3 have not been elucidated. STAT3 undergoes A-to-I RNA editing in an intron residing in proximity to the alternatively spliced exon. We hypothesized that RNA editing plays a role in regulating alternative splicing toward STAT3β. In this study we extend our observation connecting RNA editing to the preferential induction of STAT3β expression. We study the involvement of ADAR1 in STAT3 editing and reveal the connection between editing and alternative splicing of STAT3. Deferoaxamine treatment caused the induction in STAT3 RNA editing and STAT3β expression. Silencing ADAR1 caused a decrease in STAT3 editing and expression with a preferential decrease in STAT3β. Cells transfected with a mutated minigene showed preferential splicing toward the STAT3β transcript. Editing in the STAT3 intron is performed by ADAR1 and affects STAT3 alternative splicing. These results suggest that RNA editing is one of the molecular mechanisms regulating the expression of STAT3β.

  14. 30 CFR 18.43 - Explosion-proof splice boxes.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Explosion-proof splice boxes. 18.43 Section 18.43 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION... Design Requirements § 18.43 Explosion-proof splice boxes. Internal connections shall be rigidly held and...

  15. BaBar Data Aquisition

    CERN Document Server

    Scott, I; Grosso, P; Hamilton, R T; Huffer, M E; O'Grady, C; Russell, J J

    1998-01-01

    The BaBar experiment at the Stanford Linear Accelerator Center is designed to perform a search for CP violation by analysing the decays of a very large sample of B and Bbar mesons produced at the high luminosity PEP-11 accelerator. The data acquisition system must cope with a sustained high event rate, while supporting real time feature extraction and data compression with minimal dead time. The BaBar data acquisition system is based around a common VME interface to the electronics read-out of the separate detector subsystems. Data from the front end electronics is read into commercial VME processors via a custom "personality card" and PCI interface. The commercial CPUs run the Tornado operating system to provide a platform for detector subsystem code to perform the necessary data processing. The data are read out via a non-blocking network switch to a farm of commercial UNIX processors. Careful design of the core data acquisition code has enabled us to sustain events rates in excess of 20 kHz while maintaini...

  16. Some relations between two stages DNA splicing languages

    Science.gov (United States)

    Mudaber, Mohammad Hassan; Yusof, Yuhani; Mohamad, Mohd Sham

    2014-06-01

    A new symbolization of Yusof-Goode (Y-G) rule, which is associated with Y-G splicing system, was introduced by Yusof in 2012 under the framework of formal language theory. The purpose of this investigation is to present the biological process of DNA splicing in a translucent way. In this study, two stages splicing languages are introduced based on Y-G approach and some relations between stage one and stage two splicing languages are presented, given as theorems. Additionally, the existing relations between two stages splicing languages based on crossings and contexts of restriction enzymes factors with respect to two initial strings (having two cutting sites) and two rules are presented as subset.

  17. The Electrical Resistance of Rutherford-Type Superconducting Cable Splices

    CERN Document Server

    Heck, C; Fleiter, J; Bottura, L

    2015-01-01

    The electrical resistance of Large Hadron Collider main busbar cable lap splices produced by soft soldering has been measured with two independent methods as a function of intercable contact area and for splices made of cables with various defects. For defect-free lap splices, the resistance increases from 0.3 to 10 nΩ (at 4.3 K in self-field) when reducing the cable overlap length from 120 to 3 mm, as expected assuming that the resistance is inversely proportional to the intercable contact area. The resistance of bridge splices that connect side-by-side cables can be predicted from the lap splice resistances and the overlap areas involved.

  18. LHC DIPOLE MAGNET SPLICE RESISTANCE FROM SM18 DATA MINING

    CERN Document Server

    Reymond, H; Charrondiere, C; Lehmann Miotto, G; Rijllart, A; Scannicchio, D A

    2011-01-01

    The splice incident which happened during commissioning of the LHC on the 19th of September 2008 caused damage to several magnets and adjacent equipment. This raised not only the question of how it happened, but also about the state of all other splices. The inter magnet splices were immediately studied and new measurements recorded, but the internal magnet splices were still a concern. At the Chamonix meeting in January 2009, the CERN management decided to create a working group to analyse quench data of the magnet acceptance tests in an attempt to find indications for bad splices in the main dipoles. This resulted in a data-mining project that took about one year to complete. This presentation describes how the data was stored, extracted and analysed reusing existing LabVIEW™ based tools. We also present the encountered difficulties and the importance of combining measured data with operator notes in the logbook.

  19. Detecting Image Splicing Using Merged Features in Chroma Space

    Directory of Open Access Journals (Sweden)

    Bo Xu

    2014-01-01

    Full Text Available Image splicing is an image editing method to copy a part of an image and paste it onto another image, and it is commonly followed by postprocessing such as local/global blurring, compression, and resizing. To detect this kind of forgery, the image rich models, a feature set successfully used in the steganalysis is evaluated on the splicing image dataset at first, and the dominant submodel is selected as the first kind of feature. The selected feature and the DCT Markov features are used together to detect splicing forgery in the chroma channel, which is convinced effective in splicing detection. The experimental results indicate that the proposed method can detect splicing forgeries with lower error rate compared to the previous literature.

  20. RNA splicing factors as oncoproteins and tumor suppressors

    Science.gov (United States)

    Dvinge, Heidi; Kim, Eunhee; Abdel-Wahab, Omar; Bradley, Robert K.

    2016-01-01

    Preface The recent genomic characterization of cancers has revealed recurrent somatic point mutations and copy number changes affecting genes encoding RNA splicing factors. Initial studies of these ‘spliceosomal mutations’ suggest that the proteins bearing these mutations exhibit altered splice site and/or exon recognition preferences relative to their wild-type counterparts, resulting in cancer-specific mis-splicing. Such changes in the splicing machinery may create novel vulnerabilities in cancer cells that can be therapeutically exploited using compounds that can influence the splicing process. Further studies to dissect the biochemical, genomic, and biological effects of spliceosomal mutations are critical for the development of cancer therapies targeted to these mutations. PMID:27282250

  1. A TIMP-1 splice variant transcript

    DEFF Research Database (Denmark)

    Øbro, Nina Friesgård; Lademann, Ulrik Axel; Birkenkamp-Demtroder, Karin

    2008-01-01

    A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in color...... of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.......A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts...... in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2...

  2. Nuss bar migrations: occurrence and classification

    Energy Technology Data Exchange (ETDEWEB)

    Binkovitz, Lauren E.; Binkovitz, Larry A. [Mayo Clinic, Department of Radiology, Rochester, MN (United States); Zendejas, Benjamin; Moir, Christopher R. [Mayo Clinic, Department of Surgery, Rochester, MN (United States)

    2016-12-15

    Pectus excavatum results from dorsal deviation of the sternum causing narrowing of the anterior-posterior diameter of the chest. It can result in significant cosmetic deformities and cardiopulmonary compromise if severe. The Nuss procedure is a minimally invasive technique that involves placing a thin horizontally oriented metal bar below the dorsal sternal apex for correction of the pectus deformity. To identify the frequency and types of Nuss bar migrations, to present a new categorization of bar migrations, and to present examples of true migrations and pseudomigrations. We retrospectively reviewed the electronic medical records and all pertinent radiologic studies of 311 pediatric patients who underwent a Nuss procedure. We evaluated the frequency and type of bar migrations. Bar migration was demonstrated in 23 of 311 patients (7%) and occurred within a mean period of 26 days after surgery. Bar migrations were subjectively defined as deviation of the bar from the position demonstrated on the immediate postoperative radiographs and categorized as superior, inferior, rotation, lateral or flipped using a new classification system. Sixteen of the 23 migrations required re-operation. Nuss bar migration can be diagnosed with careful evaluation of serial radiographs. Nuss bar migration has a wide variety of appearances and requires exclusion of pseudomigration resulting from changes in patient positioning between radiologic examinations. (orig.)

  3. NGC 4340: Double Bar + Fossil Nuclear Ring

    OpenAIRE

    Erwin, P.; Beltran, J. C. Vega; Beckman, J. E.

    2000-01-01

    NGC 4340 is a double-barred SB0 galaxy in the Virgo cluster (Wozniak et al. 1995). Here, we present evidence that this galaxy also posseses a luminous stellar nuclear ring of relatively old stars with little or no gas. The ring lies just outside the inner bar, at the probable inner inner Lindblad resonance (IILR) of the outer bar. Careful inspection of the isophotes and unsharp masks shows that the two bars are slightly misaligned, which suggests they may be independently rotating.

  4. submitter Performance simulation of BaBar DIRC bar boxes in TORCH

    CERN Document Server

    Föhl, K; Castillo García, L; Cussans, D; Forty, R; Frei, C; Gao, R; Gys, T; Harnew, N; Piedigrossi, D; Rademacker, J; Ros García, A; van Dijk, M

    2017-01-01

    TORCH is a large-area precision time-of-flight detector based on the DIRC principle. The DIRC bar boxes of the BaBar experiment at SLAC could possibly be reused to form a part of the TORCH detector time-of-flight wall area, proposed to provide positive particle identification of low momentum kaons in the LHCb experiment at CERN. For a potential integration of BaBar bar boxes into TORCH, new imaging readout optics are required. From the several designs of readout optics that have been considered, two are used in this paper to study the effect of BaBar bar optical imperfections on the detector reconstruction performance. The kaon-pion separation powers obtained from analysing simulated photon hit patterns show the performance reduction for a BaBar bar of non-square geometry compared to a perfectly rectangular cross section.

  5. Search for CP Violation in Hyperon Decay: $\\Xi^- / \\bar{\\Xi}^+$ and $\\Lambda / \\bar{\\Lambda}$

    Energy Technology Data Exchange (ETDEWEB)

    Leros, Nicolas [Lausanne U.

    2001-06-01

    The HyperCP(EB71) experiment, performed at the Fermi National Accelerator Laboratory in the United States, provides a primary search for direct OP violation in the decays of $\\Xi^-/\\bar{\\Xi}^+$ and $\\Lambda/ \\bar{\\Lambda}$ hyperons....

  6. Health effect of a bus ride in an urban environment

    Energy Technology Data Exchange (ETDEWEB)

    Takano, T.

    1983-07-01

    Health effects on subjects during a bus drive on an urban road were investigated in a real situation. Leukocyte count, adrenalin level in blood and urine, noradrenalin and dopamine in urine, diastolic blood pressure, and index of vascular resistance increased significantly on the drive day compared with the control day. A bus drive on an urban road induced enhanced sympathetic-adreno-medullary activity in real life as a result of the comprehensive effect of various factors. The concentrations of air pollutants were excessively high on the road, and correlated with traffic volume. It was concluded that the interactions between psychogenic, physical nonspecific and physical specific effects should be taken into consideration when evaluating human health effects of air pollution in the urban environment.

  7. American Fuel Cell Bus Project Evaluation: Third Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jeffers, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2017-05-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB, which was developed as part of the Federal Transit Administration's (FTA) National Fuel Cell Bus Program, was delivered to SunLine in November 2011 and was put in revenue service in mid-December 2011. Two new AFCBs with an upgraded design were delivered in June/July of 2014 and a third new AFCB was delivered in February 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report covers the performance of the AFCBs from July 2015 through December 2016.

  8. Discharging a DC bus capacitor of an electrical converter system

    Science.gov (United States)

    Kajouke, Lateef A; Perisic, Milun; Ransom, Ray M

    2014-10-14

    A system and method of discharging a bus capacitor of a bidirectional matrix converter of a vehicle are presented here. The method begins by electrically shorting the AC interface of the converter after an AC energy source is disconnected from the AC interface. The method continues by arranging a plurality of switching elements of a second energy conversion module into a discharge configuration to establish an electrical current path from a first terminal of an isolation module, through an inductive element, and to a second terminal of the isolation module. The method also modulates a plurality of switching elements of a first energy conversion module, while maintaining the discharge configuration of the second energy conversion module, to at least partially discharge a DC bus capacitor.

  9. Heat storage for a bus petrol internal-combustion engine

    Science.gov (United States)

    Vasiliev, Leonard L.; Burak, Victor S.; Kulakov, Andry G.; Mishkinis, Donatas A.; Bohan, Pavel V.

    The heat storage (HS) system for pre-heating a bus petrol internal combustion engine to starting was mathematically modelled and experimentally investigated. The development of such devices is an extremely urgent problem especially for regions with a cold climate. We discuss how HS works on the effect of absorption and rejection of heat energy at a solid-liquid phase change of a HS substance. In the first part of the paper a numerical method to calculate the HS mass-dimensional parameters and their characteristics are described. In the experimental part of the paper results are given of experiments on the pre-heating device aiding to start a carburettor engine under operational conditions and analysis of data received. Practical confirmation of the theoretical development of HS devices for a bus engine for starting by pre-heating is given.

  10. Sustainable transport and the organisation of bus services in Manchester

    DEFF Research Database (Denmark)

    Gudmundsson, Henrik; Sørensen, Claus Hedegaard

    2008-01-01

    “Sustainable transport” has become a headline for transport planning and policy making around the world. While there are many different definitions of what Sustainable Transport means, it commonly includes efforts to improve the environmental performance of transport systems, and to promote...... depends on how the bus sector is organised. By organisation we adopt an institutional point of view and consider basic modes of governance. Basic modes of governance include the forms “market”, “hierarchy” and “network” (Powell 1990). So-called New Public Management (NPM) reforms have changed the modes......: Public bus transport in England, exemplified by Greater Manchester. England represents a situation where - compared to continental Europe including Denmark - the NPM reforms have been particularly radical. In no other European country the market forces has achieved a role as dominant as in England...

  11. Design Report for Isolated RS-485 Bus Node

    Science.gov (United States)

    2016-07-01

    1.2 Master Control Node The master control node was implemented with a USB to universal asynchronous receiver/transmitter (UART) bridge chip produced...indicate fragment manipulation via calls to public methods. The black arrows in the main activity indicate the fragments requesting action from the...Unplug the universal serial bus (USB) cable from the tablet. 2. Go to Android back stack via button on bottom right and swipe off STSLEDCntrl. 3

  12. BC Transit Fuel Cell Bus Project: Evaluation Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, M. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-02-01

    This report evaluates a fuel cell electric bus demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. This evaluation report covers two years of revenue service data on the buses from April 2011 through March 2013.

  13. Evaluasi Tingkat Kelelahan Pada Pengemudi Bus Di Kota Bandung

    OpenAIRE

    Febrianti, Asterina; Yassierli, Yassierli; Mahachandra, Manik

    2016-01-01

    Kota Bandung saat ini mengalami pertumbuhan penduduk mencapai 3% setiap tahunnya, sehingga kebutuhan akan transportasi umum sangat tinggi serta tingkat kemacetan yang timbul semakin tinggi pula. Kemacetan yang panjang berdampak pada penurunan kewaspadaan dan timbulnya risiko kecelakaan karena waktu berkendara yang relatif lebih beragam. Menyadari akan risiko kecelakaan yang diakibatkan oleh kelelahan maka diperlukan pengukuran kelelahan pada pengemudi bus di kota Bandung yang belum pernah dik...

  14. City of Chula Vista hydrogen fuel cell bus demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, B.; Bamberger, B.

    1996-10-01

    Hydrogen as an energy carrier and fuel has potential for various uses including electricity, commercial, residential, transportation, and industrial. It is an energy carrier that can be produced from a variety of primary sources and potentially can accomplish these various uses while significantly reducing pollution by substituting for or reducing the use of fossil fuels. One of the most immediate and potentially viable roles for hydrogen as an energy carrier will be its use as a transportation fuel, especially in densely populated urban areas where automotive emissions contribute significantly to air pollution. The Department of Energy`s commitment to research and development of hydrogen as an alternative fuel, and California`s Zero Emission Vehicle (ZEV) requirements, both provide the impetus and favorable circumstance for demonstrating hydrogen as a transportation fuel on an urban bus system. The purpose of this project is to demonstrate the feasibility of using solid polymer fuel cells in a hydrogen-powered electric drive system for an urban transit bus application. Fuel cell buses use hydrogen fuel and oxygen from the air to produce electrical power with the only byproduct being pure water. Proton Exchange Membrane (PEM) fuel cells are proposed for this project. Current evidence suggests that fuel cells, which rely on hydrogen and a process known as proton exchange to generate their power, appear to have an infinite life span. All exhaust pollution is completely eliminated, resulting in a Zero Emission Vehicle (ZEV). An urban bus system offers the potential for developing a market for the production of hydrogen propulsion technology due to extensive vehicular use in densely populated areas experiencing pollution from numerous sources, and because the central garaging facilities or the bus system facilitates fueling and maintenance functions.

  15. Bi-Directional Multi-Station Command/Information Bus

    Science.gov (United States)

    1976-11-01

    signals are presented. The model of the signal flow requirements is presented both schematically and in tabular form From this model, a possible...is presented both in schematic and tabular forms. Finally, a conceptual data bus configura- tion meeting the signal flow requirements has been...A Converter LRI Central Air Dato Computer ’LLI Demultiplexer D/A Converter 0>AI OfA2 Type R Modem Type T Modem A/D Cowerfcr

  16. Optimalisasi Kelembagaan Dan Manajemen Dalam Bus Rapid Transit Semarang

    OpenAIRE

    Ghoni, Ahmad; Yuniar, Haptiwi Tri; Riyanto, Bambang; Supriyono, Supriyono

    2013-01-01

    Bus Rapid Transit Trans Semarang is a means of mass transportation to the transit system that began operating since 2009 as a solution to reduce congestion in the city of Semarang. This research focuses on the optimization and management of institutional BRT Trans Semarang to improve it's performance. The purpose of this study is to optimize the performance of the organization and management of BRT Trans Semarang with variable types of institutional cooperation BRT operations, and leadership...

  17. Effects of biodiesel on emissions of a bus diesel engine

    OpenAIRE

    Kegl, Breda

    2012-01-01

    This paper discusses the influence of biodiesel on the injection, spray, and engine characteristics with the aim to reduce harmful emissions. The considered engine is a bus diesel engine with injection M system. The injection, fuel spray, and engine characteristics, obtained with biodiesel, are compared to those obtained with mineral diesel (D2) under various operating regimes. The considered fuel is neat biodiesel from rapeseed oil. Its density, viscosity, surface tension, and sound velocity...

  18. Experimental investigation review of biodiesel usage in bus diesel engine

    OpenAIRE

    Kegl, Breda; Kegl, Marko

    2017-01-01

    This paper assembles and analyses extensive experimental research work conducted for several years in relation to biodiesel usage in a MAN bus Diesel engine with M injection system. At first the most important properties of the actually used neat rapeseed biodiesel fuel and its blends with mineral diesel are discussed and compared to that of mineral diesel. Then the injection, fuel spray, and engine characteristics for various considered fuel blends are compared at various ambient conditions,...

  19. Should bus commuting be subsidized for providing quality transport ...

    Indian Academy of Sciences (India)

    e-mail: geetamt@gmail.com. Abstract. Urban transport has ... (ii) Should bus prices be subsidized in order to provide a quality public transport system? (iii) How large is the modal shift in favour ..... been derived through a speed-flow relationship function, with a car free flow speed of 45 km/h, peak traffic speed of 24 km/h and ...

  20. Bar pattern speeds in CALIFA galaxies. I. Fast bars across the Hubble sequence

    NARCIS (Netherlands)

    Aguerri, J. A. L.; Méndez-Abreu, J.; Falcón-Barroso, J.; Amorin, A.; Barrera-Ballesteros, J.; Cid Fernandes, R.; García-Benito, R.; García-Lorenzo, B.; González Delgado, R. M.; Husemann, B.; Kalinova, V.; Lyubenova, M.; Marino, R. A.; Márquez, I.; Mast, D.; Pérez, E.; Sánchez, S. F.; van de Ven, G.; Walcher, C. J.; Backsmann, N.; Cortijo-Ferrero, C.; Bland-Hawthorn, J.; del Olmo, A.; Iglesias-Páramo, J.; Pérez, I.; Sánchez-Blázquez, P.; Wisotzki, L.; Ziegler, B.

    Context. The bar pattern speed (Ωb) is defined as the rotational frequency of the bar, and it determines the bar dynamics. Several methods have been proposed for measuring Ωb. The non-parametric method proposed by Tremaine & Weinberg (1984, ApJ, 282, L5; TW) and based on stellar kinematics is the

  1. Bank pull or bar push: What drives scroll-bar formation in meandering rivers?

    NARCIS (Netherlands)

    van de Lageweg, W. I.; van Dijk, W. M.; Baar, A. W.; Rutten, J.; Kleinhans, M. G.

    2014-01-01

    One of the most striking features of meandering rivers are quasi-regular ridges of the point bar, evidence of a pulsed lateral migration of meander bends. Scroll bars formed on the inner bend are preserved on the point-bar surface as a series of ridges as meanders migrate, and in the subsurface of

  2. Common Traffic Violations of Bus Drivers in Urban China: An Observational Study.

    Directory of Open Access Journals (Sweden)

    Qiqi Wang

    Full Text Available To report common traffic violations in bus drivers and the factors that influence those violations in urban China.We conducted an observational study to record three types of traffic violations among bus drivers in Changsha City, China: illegal stopping at bus stations, violating traffic light signals, and distracted driving. The behaviors of bus drivers on 32 routes (20% of bus routes in the city were observed. A two-level Poisson regression examined factors that predicted bus driver violations.The incidence of illegal stopping at bus stations was 20.2%. Illegal stopping was less frequent on weekends, sunny days, and at stations with cameras, with adjusted incidence rate ratios (IRRs of 0.81, 0.65 and 0.89, respectively. The incidence of violating traffic light signals was 2.2%, and was lower on cloudy than sunny days (adjusted IRR: 0.60. The incidence of distracted driving was 3.3%. The incidence of distracted driving was less common on cloudy days, rainy or snowy days, and foggy/windy/dusty days compared to sunny days, with adjusted IRRs of 0.54, 0.55 and 0.07, respectively.Traffic violations are common in bus drivers in urban China and they are associated with the date, weather, and presence of traffic cameras at bus station. Further studies are recommended to understand the behavioral mechanisms that may explain bus driver violations and to develop feasible prevention measures.

  3. Evaluation of fatal school bus related crashes and near-term crash mitigation strategies

    Directory of Open Access Journals (Sweden)

    Kelly Donoughe

    2015-03-01

    Full Text Available School bus crashes are rare in comparison to other crash types, but considering all crashes that occur in and around school buses, they begin to become a noticeable problem and one that tends to attract national attention. As defined by the Fatality Analysis Reporting System (FARS, a school bus related crash is a crash that either involves a school bus or a crash where the presence of a school bus is considered as a major contributing factor. Ten years of data indicate that the number of fatal school bus related crashes has remained nearly stagnant despite an increase in the number vehicle safety systems available on the market. The findings also highlight the importance of protecting the non-bus occupants since they are the most likely to incur a serious or fatal injury in the event of a crash. As the most vulnerable user group, pedestrians (typically school-aged children are especially at risk when crossing the road while boarding or exiting a school bus. Until new technologies for reducing school bus related crashes are designed and implemented, school transportation safety can be improved by increasing awareness of school bus stop laws and by implementing existing transportation safety initiatives at school bus stop locations.

  4. Cellular Automata Based Modeling for Evaluating Different Bus Stop Designs in China

    Directory of Open Access Journals (Sweden)

    Haoyang Ding

    2015-01-01

    Full Text Available A cellular automaton model is proposed to simulate mixed traffic flow composed of motor vehicles and bicycles near bus stops. Three typical types of bus stops which are common in China are considered in the model, including two types of curbside bus stops and one type of bus bay stops. Passenger transport capacity of three types of bus stops, which is applied to evaluate the bus stop design, is calculated based on the corresponding traffic flow rate. According to the simulation results, the flow rates of both motor vehicles and bicycles exhibit phase transition from free flow to the saturation one at the critical point. The results also show that the larger the interaction between motor vehicle and bicycle flow is near curbside bus stops, the more the value of saturated flows drops. Curbside bus stops are more suitable when the conflicts between two flows are small and the inflow rate of motor vehicles is low. On the contrary, bus bay stops should be applied due to their ability to reduce traffic conflicts. Findings of this study can provide useful suggestions on bus stop selection considering different inflow rate of motor vehicles and bicycles simultaneously.

  5. HEV Test Bench Based on CAN Bus Sensor Communication

    Directory of Open Access Journals (Sweden)

    Shupeng ZHAO

    2014-02-01

    Full Text Available The HEV test bench based on Controller Area Network bus was studied and developed. Control system of HEV power test bench used the CAN bus technology. The application of CAN bus technology on control system development has opened up a new research direction for domestic automobile experimental platform. The HEV power control system development work was completed, including power master controller, electric throttle controller, driving simulation platform, CAN2.0 B communication protocol procedures for formulation, CAN communication monitoring system, the simulation model based on MATLAB code automatic generation technology research, etc. Maximum absorption power of the test bench is 90 kW, the test bench top speed is 6000 r/min, the CAN communication data baud rate is 10~500 k, the conventional electric measurement parameter part precision satisfies the requirement of development of HEV. On the HEV test bench the result of regenerative braking experiment shows that the result got by the test bench was closer to the results got by outdoor road test. And the fuel consumption experiment test results show that the HEV fuel consumption and the charge-discharge character are in linear relationship. The establishment of the test platform for the evaluation of the development of hybrid electric vehicle and power provides physical simulation and test platform.

  6. Evaluation of Bus Vibration Comfort Based on Passenger Crowdsourcing Mode

    Directory of Open Access Journals (Sweden)

    Hong Zhao

    2016-01-01

    Full Text Available Vibration comfort is an important factor affecting the quality of service (QoS of bus. In order to make people involved in supervising bus’s vibration comfort and improve passengers’ riding experience, a novel mode of passenger crowdsourcing is introduced. In this paper, comfort degree of bus vibration is calculated from bus’s vibration signals collected by passengers’ smartphones and sent through WiFi to the Boa web server which shows the vibration comfort on the LCD deployed in bus and maybe trigger alarm lamp when the vibration is beyond the threshold. Three challenges here have been overcome: firstly, space coordinate transformation algorithm is used to solve the constant drift of signals collected; secondly, a low-pass filter is designed to isolate gravity from signals real-timely via limited computing resources; thirdly, an embedded evaluation system is developed according to the calculation procedure specified by criterion ISO 2631-1997. Meanwhile, the model proposed is tested in a practical running environment, the vibration data in whole travel are recorded and analyzed offline. The results show that comfort degree of vibration obtained from the experimental system is identical with the truth, and this mode is proved to be effective.

  7. Replacement model of city bus: A dynamic programming approach

    Science.gov (United States)

    Arifin, Dadang; Yusuf, Edhi

    2017-06-01

    This paper aims to develop a replacement model of city bus vehicles operated in Bandung City. This study is driven from real cases encountered by the Damri Company in the efforts to improve services to the public. The replacement model propounds two policy alternatives: First, to maintain or keep the vehicles, and second is to replace them with new ones taking into account operating costs, revenue, salvage value, and acquisition cost of a new vehicle. A deterministic dynamic programming approach is used to solve the model. The optimization process was heuristically executed using empirical data of Perum Damri. The output of the model is to determine the replacement schedule and the best policy if the vehicle has passed the economic life. Based on the results, the technical life of the bus is approximately 20 years old, while the economic life is an average of 9 (nine) years. It means that after the bus is operated for 9 (nine) years, managers should consider the policy of rejuvenation.

  8. An Electric Bus with a Battery Exchange System

    Directory of Open Access Journals (Sweden)

    Jeongyong Kim

    2015-07-01

    Full Text Available As part of the ongoing effort to be independent of petroleum resources and to be free from pollutant emission issues, various electric vehicles have been developed and tested through their integration with real world systems. In the current paper, yet another application specific EV for public transportation, an electric bus, is introduced and explained with results from the pilot test program which was carried out under real traffic conditions. The main feature of the current system is a battery exchanging mechanism mounted on the roof of the bus. The current configuration certainly requires an externally fabricated battery exchanging robot system that would complement the electric bus for a fully automated battery exchanging process. The major advantage of the current system is the quick re-charging of the electric energy through the physical battery exchange and the possible utilization of the battery exchange station as a mini scale energy storage system for grid system peak power shaving. With the total system solution approach for the public transportation system, it is fully expected to create outstanding business opportunities in number of areas such as battery suppliers, battery exchanging station management, battery leasing and many more.

  9. A Dimensional Bus model for integrating clinical and research data.

    Science.gov (United States)

    Wade, Ted D; Hum, Richard C; Murphy, James R

    2011-12-01

    Many clinical research data integration platforms rely on the Entity-Attribute-Value model because of its flexibility, even though it presents problems in query formulation and execution time. The authors sought more balance in these traits. Borrowing concepts from Entity-Attribute-Value and from enterprise data warehousing, the authors designed an alternative called the Dimensional Bus model and used it to integrate electronic medical record, sponsored study, and biorepository data. Each type of observational collection has its own table, and the structure of these tables varies to suit the source data. The observational tables are linked to the Bus, which holds provenance information and links to various classificatory dimensions that amplify the meaning of the data or facilitate its query and exposure management. The authors implemented a Bus-based clinical research data repository with a query system that flexibly manages data access and confidentiality, facilitates catalog search, and readily formulates and compiles complex queries. The design provides a workable way to manage and query mixed schemas in a data warehouse.

  10. Safety evaluation of a hydrogen fueled transit bus

    Energy Technology Data Exchange (ETDEWEB)

    Coutts, D.A.; Thomas, J.K.; Hovis, G.L.; Wu, T.T. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1997-12-31

    Hydrogen fueled vehicle demonstration projects must satisfy management and regulator safety expectations. This is often accomplished using hazard and safety analyses. Such an analysis has been completed to evaluate the safety of the H2Fuel bus to be operated in Augusta, Georgia. The evaluation methods and criteria used reflect the Department of Energy`s graded approach for qualifying and documenting nuclear and chemical facility safety. The work focused on the storage and distribution of hydrogen as the bus motor fuel with emphases on the technical and operational aspects of using metal hydride beds to store hydrogen. The safety evaluation demonstrated that the operation of the H2Fuel bus represents a moderate risk. This is the same risk level determined for operation of conventionally powered transit buses in the United States. By the same criteria, private passenger automobile travel in the United States is considered a high risk. The evaluation also identified several design and operational modifications that resulted in improved safety, operability, and reliability. The hazard assessment methodology used in this project has widespread applicability to other innovative operations and systems, and the techniques can serve as a template for other similar projects.

  11. Perspective Application of Passive Optical Network with Optimized Bus Topology

    Directory of Open Access Journals (Sweden)

    P. Lafata

    2012-06-01

    Full Text Available Passive optical networks (PONs represent a promising solution for modern access telecommunication networks.These networks are able to meet the increasing demands on transmission rate for demanding multimedia services,while they can offer typical shared transmission speed of 1.25 or 2.5 Gbps. The major role in deploying opticaldistribution networks ODNs plays the maximum attenuable loss, which is caused mainly by passive optical splitters.This paper proposes an innovative application of passive optical networks with optimized bus topology especially forlocal backbone data networks. Due to using only passive components, it is necessary to optimize certain parameters,especially an overall attenuation balance. Considering the possibility of such optimization, the passive optical networkwith optimized bus topology provides several interesting opportunities for specific applications. This paper will presentselected aspects of passive optical networks and splitters with asymmetric splitting ratio. The essential part is focusedon the practical demonstration of their use to optimize the passive optical network with bus topology, which acts as alocal backbone network for structured cabling systems, and for local data networks in large buildings.

  12. Regulation of plant developmental processes by a novel splicing factor.

    Science.gov (United States)

    Ali, Gul Shad; Palusa, Saiprasad G; Golovkin, Maxim; Prasad, Jayendra; Manley, James L; Reddy, Anireddy S N

    2007-05-30

    Serine/arginine-rich (SR) proteins play important roles in constitutive and alternative splicing and other aspects of mRNA metabolism. We have previously isolated a unique plant SR protein (SR45) with atypical domain organization. However, the biological and molecular functions of this novel SR protein are not known. Here, we report biological and molecular functions of this protein. Using an in vitro splicing complementation assay, we showed that SR45 functions as an essential splicing factor. Furthermore, the alternative splicing pattern of transcripts of several other SR genes was altered in a mutant, sr45-1, suggesting that the observed phenotypic abnormalities in sr45-1 are likely due to altered levels of SR protein isoforms, which in turn modulate splicing of other pre-mRNAs. sr45-1 exhibited developmental abnormalities, including delayed flowering, narrow leaves and altered number of petals and stamens. The late flowering phenotype was observed under both long days and short days and was rescued by vernalization. FLC, a key flowering repressor, is up-regulated in sr45-1 demonstrating that SR45 influences the autonomous flowering pathway. Changes in the alternative splicing of SR genes and the phenotypic defects in the mutant were rescued by SR45 cDNA, further confirming that the observed defects in the mutant are due to the lack of SR45. These results indicate that SR45 is a novel plant-specific splicing factor that plays a crucial role in regulating developmental processes.

  13. Low resistance splices for HTS devices and applications

    Science.gov (United States)

    Lalitha, S. L.

    2017-09-01

    This paper discusses the preparation methodology and performance evaluation of low resistance splices made of the second generation (2G) high-temperature superconductor (HTS). These splices are required in a broad spectrum of HTS devices including a large aperture, high-field solenoid built in the laboratory to demonstrate a superconducting magnetic energy storage (SMES) device. Several pancake coils are assembled in the form of a nested solenoid, and each coil requires a hundred meters or more of 2G (RE)BCO tape. However, commercial availability of this superconductor with a very uniform physical properties is currently limited to shorter piece lengths. This necessitates us having splices to inter-connect the tape pieces within a pancake coil, between adjacent pancake coils, and to attach HTS current leads to the magnet assembly. As a part of the optimization and qualification of splicing process, a systematic study was undertaken to analyze the electrical performance of splices in two different configurations suitable for this magnet assembly: lap joint and spiral joint. The electrical performance is quantified in terms of the resistance of splices estimated from the current-voltage characteristics. It has been demonstrated that a careful application of this splicing technique can generate lap joints with resistance less than 1 nΩ at 77 K.

  14. Herboxidiene triggers splicing repression and abiotic stress responses in plants

    KAUST Repository

    Alshareef, Sahar

    2017-03-27

    Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.

  15. Aberrant and alternative splicing in skeletal system disease.

    Science.gov (United States)

    Fan, Xin; Tang, Liling

    2013-10-01

    The main function of skeletal system is to support the body and help movement. A variety of factors can lead to skeletal system disease, including age, exercise, and of course genetic makeup and expression. Pre-mRNA splicing plays a crucial role in gene expression, by creating multiple protein variants with different biological functions. The recent studies show that several skeletal system diseases are related to pre-mRNA splicing. This review focuses on the relationship between pre-mRNA splicing and skeletal system disease. On the one hand, splice site mutation that leads to aberrant splicing often causes genetic skeletal system disease, like COL1A1, SEDL and LRP5. On the other hand, alternative splicing without genomic mutation may generate some marker protein isoforms, for example, FN, VEGF and CD44. Therefore, understanding the relationship between pre-mRNA splicing and skeletal system disease will aid in uncovering the mechanism of disease and contribute to the future development of gene therapy. © 2013 Elsevier B.V. All rights reserved.

  16. Alternative splicing regulation of APP exon 7 by RBFox proteins.

    Science.gov (United States)

    Alam, Shafiul; Suzuki, Hitoshi; Tsukahara, Toshifumi

    2014-12-01

    RBFox proteins are well-known alternative splicing regulators. We have shown previously that during neuronal differentiation of P19 cells induced by all-trans retinoic acid and cell aggregation, RBFox1 shows markedly increased temporal expression. To find its key splicing regulation, we examined the effect of RBFox1 on 33 previously reported and validated neuronal splicing events of P19 cells. We observed that alternative splicing of three genes, specifically, amyloid precursor protein (APP), disks large homolog 3 (DLG3), and G protein, alpha activating activity polypeptide O (GNAO1), was altered by transient RBFox1 expression in HEK293 and HeLa cells. Moreover, an RBFox1 mutant (RBFox1FA) that was unable to bind the target RNA sequence ((U)GCAUG) did not induce these splicing events. APP generates amyloid beta peptides that are involved in the pathology of Alzheimer's disease, and therefore we examined APP alternative splicing regulation by RBFox1 and other splicing regulators. Our results indicated that RBFox proteins promote the skipping of APP exon 7, but not the inclusion of exon 8. We made APP6789 minigenes and observed that two (U)GCAUG sequences, located upstream of exon 7 and in exon 7, functioned to induce skipping of exon 7 by RBFox proteins. Overall, RBFox proteins may shift APP from exon 7 containing isoforms, APP770 and APP751, toward the exon 7 lacking isoform, APP695, which is predominant in neural tissues. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Stabilizer bars: Part 3. Production methods

    Directory of Open Access Journals (Sweden)

    Adam-Markus WITTEK

    2011-01-01

    Full Text Available The article outlines the methods of stabilizer bar production as well as selected issues concerning the theory of cold roll forming. A proper construction and the selection of parameters are fundamental factors influencing the selection of a suitable production process. The selection of the manufacturing process has a fundamental impact on the quality and durability of the stabilizer bars.

  18. Development of an effective pinch bar

    CSIR Research Space (South Africa)

    Ottermann, RW

    2003-02-01

    Full Text Available an impact in addressing rock fall fatalities and injuries associated with making safe or barring activities. The tools will also improve the overall quality of barring, especially in excavations requiring a lot of work, and this will reduce the rock fall...

  19. Bar Study Stories. Issues in Prevention

    Science.gov (United States)

    Higher Education Center for Alcohol, Drug Abuse, and Violence Prevention, 2012

    2012-01-01

    This issue of "Issues in Prevention" focuses on the impact of the availability of drinks in licensed establishments, such as bars and taverns on student drinking. This issue contains the following articles: (1) Cheap Drinks at College Bars Can Escalate Student Drinking (John D. Clapp); (2) High Alcohol Outlet Density: A Problem for Campuses and…

  20. Oriented scanning is the leading mechanism underlying 5' splice site selection in mammals

    NARCIS (Netherlands)

    Borensztajn, Keren; Sobrier, Marie-Laure; Duquesnoy, Philippe; Fischer, Anne-Marie; Tapon-Bretaudière, Jacqueline; Amselem, Serge

    2006-01-01

    Splice site selection is a key element of pre-mRNA splicing. Although it is known to involve specific recognition of short consensus sequences by the splicing machinery, the mechanisms by which 5' splice sites are accurately identified remain controversial and incompletely resolved. The human F7

  1. Characterization of Bars Induced by Interactions

    Directory of Open Access Journals (Sweden)

    Inma Martinez-Valpuesta

    2016-05-01

    Full Text Available Whether the formation of bars is triggered by interactions or by internal processes has been discussed for many decades. In this work, we study differences between both mechanisms by means of numerical simulations. We relate our analysis to fly-by interactions in different mass groups or clusters according to the velocity of the encounters. We find that once the bar is created, the interaction does not much affect its evolution. We also find that bars can be triggered purely by a slow interaction. Those bars affected or triggered by interaction stay for a longer time in the slow regime, i.e., the corotation radius is more than 1.4 times the bar radius.

  2. Orbits in weak and strong bars

    CERN Document Server

    Contopoulos, George

    1980-01-01

    The authors study the plane orbits in simple bar models embedded in an axisymmetric background when the bar density is about 1% (weak), 10% (intermediate) or 100% (strong bar) of the axisymmetric density. Most orbits follow the stable periodic orbits. The basic families of periodic orbits are described. In weak bars with two Inner Lindblad Resonances there is a family of stable orbits extending from the center up to the Outer Lindblad Resonance. This family contains the long period orbits near corotation. Other stable families appear between the Inner Lindblad Resonances, outside the Outer Lindblad Resonance, around corotation (short period orbits) and around the center (retrograde). Some families become unstable or disappear in strong bars. A comparison is made with cases having one or no Inner Lindblad Resonance. (12 refs).

  3. Splicing landscape of the eight collaborative cross founder strains.

    Science.gov (United States)

    Zheng, Christina L; Wilmot, Beth; Walter, Nicole Ar; Oberbeck, Denesa; Kawane, Sunita; Searles, Robert P; McWeeney, Shannon K; Hitzemann, Robert

    2015-02-05

    The Collaborative Cross (CC) is a large panel of genetically diverse recombinant inbred mouse strains specifically designed to provide a systems genetics resource for the study of complex traits. In part, the utility of the CC stems from the extensive genome-wide annotations of founder strain sequence and structural variation. Still missing, however, are transcriptome-specific annotations of the CC founder strains that could further enhance the utility of this resource. We provide a comprehensive survey of the splicing landscape of the 8 CC founder strains by leveraging the high level of alternative splicing within the brain. Using deep transcriptome sequencing, we found that a majority of the splicing landscape is conserved among the 8 strains, with ~65% of junctions being shared by at least 2 strains. We, however, found a large number of potential strain-specific splicing events as well, with an average of ~3000 and ~500 with ≥3 and ≥10 sequence read coverage, respectively, within each strain. To better understand strain-specific splicing within the CC founder strains, we defined criteria for and identified high-confidence strain-specific splicing events. These splicing events were defined as exon-exon junctions 1) found within only one strain, 2) with a read coverage ≥10, and 3) defined by a canonical splice site. With these criteria, a total of 1509 high-confidence strain-specific splicing events were identified, with the majority found within two of the wild-derived strains, CAST and PWK. Strikingly, the overwhelming majority, 94%, of these strain-specific splicing events are not yet annotated. Strain-specific splicing was also located within genomic regions recently reported to be over- and under-represented within CC populations. Phenotypic characterization of CC populations is increasing; thus these results will not only aid in further elucidating the transcriptomic architecture of the individual CC founder strains, but they will also help in guiding

  4. Alternative splicing variations in mouse CAPS2: differential expression and functional properties of splicing variants

    Directory of Open Access Journals (Sweden)

    Furuichi Teiichi

    2007-04-01

    Full Text Available Abstract Background Ca2+-dependent activator protein 2 (CAPS2/CADPS2 is a secretory vesicle-associated protein involved in the release of neurotrophin. We recently reported that an aberrant, alternatively spliced CAPS2 mRNA that lacks exon 3 (CAPS2Δexon3 is detected in some patients with autism. Splicing variations in mouse CAPS2 and their expression and functions remain unclear. Results In this study, we defined 31 exons in the mouse CAPS2 gene and identified six alternative splicing variants, CAPS2a-f. CAPS2a is an isoform lacking exons 22 and 25, which encode part of the Munc13-1-homologous domain (MHD. CAPS2b lacks exon 25. CAPS2c lacks exons 11 and 22. CAPS2d, 2e, and 2f have C-terminal deletions from exon 14, exon 12, and exon 5, respectively. On the other hand, a mouse counterpart of CAPS2Δexon3 was not detected in the mouse tissues tested. CAPS2b was expressed exclusively in the brain, and the other isoforms were highly expressed in the brain, but also in some non-neural tissues. In the brain, all isoforms showed predominant expression patterns in the cerebellum. In the developing cerebellum, CAPS2b showed an up-regulated expression pattern, whereas the other isoforms exhibited transiently peaked expression patterns. CAPS2 proteins were mostly recovered in soluble fractions, but some were present in membrane fractions, except for CAPS2c and 2f, both of which lack the PH domain, suggesting that the PH domain is important for membrane association. In contrast to CAPS2a and 2b, CAPS2c showed slightly decreased BDNF-releasing activity, which is likely due to the C-terminal truncation of the PH domain in CAPS2c. Conclusion This study indicates that, in mouse, there are six splicing variants of CAPS2 (CAPS2a-f, and that these are subdivided into two groups: a long form containing the C-terminal MHD and a short form lacking the C-terminal MHD. These results demonstrate that the splicing variations correlate with their expression patterns and

  5. Measurement of the asymmetry parameter for $\\bar\\Lambda$ in $J/\\psi \\to \\Lambda\\bar{\\Lambda}$

    CERN Document Server

    ,

    2009-01-01

    Based on a sample of $58\\times10^6J/\\psi$ decays collected with the BESII detector at the BEPC, the $\\bar\\Lambda$ decay parameter $\\alpha_{\\bar\\Lambda}$ for $\\bar\\Lambda\\to \\bar p \\pi^+$ is measured using about 9000 $J/\\psi\\to\\Lambda\\bar\\Lambda\\to p \\bar p \\pi^+\\pi^-$ decays. A fit to the joint angular distributions yields $\\alpha_{\\bar\\Lambda}(\\bar\\Lambda\\to \\bar p\\pi^+)=-0.755\\pm0.083\\pm0.063$, where the first error is statistical, and the second systematic.

  6. Identification of voltage stability condition of a power system using measurements of bus variables

    Directory of Open Access Journals (Sweden)

    Durlav Hazarika

    2014-12-01

    Full Text Available Several online methods were proposed for investigating the voltage stability condition of an interconnected power system using the measurements of voltage and current phasors at a bus. For this purpose, phasor measurement units (PMUs are used. A PMU is a device which measures the electrical waves on an electrical network, using a common time source (reference bus for synchronisation. This study proposes a method for online monitoring of voltage stability condition of a power system using measurements of bus variables namely – (i real power, (ii reactive power and (iii bus voltage magnitude at a bus. The measurements of real power, reactive power and bus voltage magnitude could be extracted/captured from a smart energy meter. The financial involvement for implementation of the proposed method would significantly lower compared with the PMU-based method.

  7. The effect of an active steering system on city bus drivers muscle activity.

    Science.gov (United States)

    Ahlström, Christer; Gink Lövgren, Maria; Nilsson, Mats; Willstrand, Tania; Anund, Anna

    2018-02-26

    City bus drivers spend hours driving under time pressure, in congested traffic and in a monotonous sitting position. This leads to unhealthy working conditions, especially in terms of physical and psychological stress. The aim of this study is to investigate if an active steering system can alleviate the musculoskeletal stress involved in manoeuvring a bus. Twenty bus drivers drove a city bus equipped with the Volvo dynamic steering (VDS) support system in real traffic. Steering effort was evaluated with fyography and with a questionnaire. Compared to baseline, VDS significantly reduced the required muscle activity by on average 15 - 25 % while turning, and with up to 68 % in the part of the manoeuvre requiring maximum effort. The bus drivers believed that VDS will help reduce neck and shoulder problems, and they expressed a desire to have VDS installed in their own bus.

  8. Bitmap-Wise Wireless M-Bus Coordination for Sustainable Real Time Energy Management

    Directory of Open Access Journals (Sweden)

    Kwang-il Hwang

    2014-07-01

    Full Text Available Even though WM-Bus is being considered to be the most promising network protocol for smart metering, it is not suitable for a sustainable real-time home energy management system (HEMS, which requires higher reliability and longer lifetime despite real time bi-directional communications. Therefore, in this paper we propose a Bitmap-wise WM-Bus (BWM-Bus, coping well with sustainable real-time HEMS. In particular, the proposed scheme addresses the several problems in WM-Bus for HEMS by introducing novel functions: asynchronous meter trigger, adaptive slot scheduling, and bitmap-wise retransmission request. Through experiments, we demonstrate that BWM-Bus guarantees higher data success ratio with lower data aggregation time, as well as longer lifetime than WM-Bus standard.

  9. Bunching transition in a time-headway model of a bus route.

    Science.gov (United States)

    Nagatani, T

    2001-03-01

    A time-headway model is presented to mimic bus behavior on the bus route. The motion of a bus is described in terms of the time headway between its bus and the bus in front. We study the bunching behavior of buses induced by interacting with other buses and passengers. It is shown that the dynamical phase transitions among the inhomogeneous bunching phase, the homogeneous free phase, the coexisting phase, and the homogeneous congested phase occur with varying the initial time headway. We study the effect of not stopping at bus stops on the time-headway profile. It is found that the bunching transition lines are consistent with the neutral stability curves obtained by the linear stability analysis.

  10. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Third Results Reports

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2012-05-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. NREL has previously published two reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from July 2011 through January 2012.

  11. Optimizing Bus Frequencies under Uncertain Demand: Case Study of the Transit Network in a Developing City

    OpenAIRE

    Huang, Zhengfeng; Ren, Gang; Liu, Haixu

    2013-01-01

    Various factors can make predicting bus passenger demand uncertain. In this study, a bilevel programming model for optimizing bus frequencies based on uncertain bus passenger demand is formulated. There are two terms constituting the upper-level objective. The first is transit network cost, consisting of the passengers’ expected travel time and operating costs, and the second is transit network robustness performance, indicated by the variance in passenger travel time. The second term reflect...

  12. Evaluation of Urban Bus Transport Failure in Gujranwala City of Pakistan

    Directory of Open Access Journals (Sweden)

    MUHAMMAD WAQAR ASLAM

    2017-07-01

    Full Text Available This research work has evaluated the performance of urban public bus transport service operating in Gujranwala city of Pakistan. The assessment has been made in terms of financial viability, bus condition, operational performance and user perception. Based on the data requirements, various traffic/transport surveys were conducted including Passenger Boarding and Alighting Survey, Bus Count Survey, Bus Inspection Survey, Illegal Operations Survey and Public Transport User Interview Survey in consultation with concerned department/agencies. Field surveys were also conducted to get input from passengers and local people. Initial investigation informed that out of four operational bus routes, only two routes are profitable. The overall bus operation is running in loss (only 0.3% net profit. It has barely any profit for the bus operator against its investment thereby making the urban bus operation financially non-viable. The whole revenue flow is dependent on passenger ticketing revenue only. Around 35% of the buses are in poor condition with major issues of quality of tyres, no speedometer, worse condition of seats, floor and ceiling, and display of route. Illegal operation of Qingqis/Rickshaws (as para-transit mode along the bus routes is also decreasing the revenue by capturing the passenger volume. According to passenger opinion analysis, around 55% of the passenger are not satisfied with the service quality and prefer other modes of transport. Their major concerns are absence of facilities for disables and bus stops, bus seating and standing capacity, poor bus condition and pick pocketing. Collectively, these factors are contributing

  13. Regulation of Apoptosis by Alternative Pre-mRNA Splicing

    National Research Council Canada - National Science Library

    Schwerk, Christian; Schulze-Osthoff, Klaus

    2005-01-01

    .... A large number of apoptotic factors are regulated via alternative splicing, a process that allows for the production of discrete protein isoforms with often distinct functions from a common mRNA precursor...

  14. Dynamic integration of splicing within gene regulatory pathways

    National Research Council Canada - National Science Library

    Braunschweig, Ulrich; Gueroussov, Serge; Plocik, Alex M; Graveley, Brenton R; Blencowe, Benjamin J

    2013-01-01

    .... In addition to generating vast repertoires of RNAs and proteins, splicing has a profound impact on other gene regulatory layers, including mRNA transcription, turnover, transport, and translation...

  15. An Interactive Wireless Communication System for Visually Impaired People Using City Bus Transport

    Directory of Open Access Journals (Sweden)

    Hsiao-Lan Wang

    2014-04-01

    Full Text Available Visually impaired people have difficulty accessing information about public transportation systems. Several systems have been developed for assisting visually impaired and blind people to use the city bus. Most systems provide only one-way communication and require high-cost and complex equipment. The purpose of this study is to reduce the difficulties faced by visually impaired people when taking city buses, using an interactive wireless communication system. The system comprised a user module and a bus module to establish a direct one-to-one connection. When the user inputs 4-digit numbers, the user module immediately sends out the information. If the bus module receives the matched bus number, it buzzes and the warning LED flashes to notify the bus driver that someone is waiting to board on the bus. User tests were conducted by two visually impaired people in a simulated vehicle and a city bus. The success rate of interactive wireless communication, recognizing the arrival of the bus and boarding the correct bus reached 100% in all of the tests. The interactive wireless communication aid system is a valid and low-cost device for assisting visually impaired people to use city buses.

  16. A Prediction System Using a P2P Overlay Network for a Bus Arrival System

    Directory of Open Access Journals (Sweden)

    Ssu-Hsuan Lu

    2014-01-01

    Full Text Available Along with the evolution of times and the surge of metropolitan populations, government agencies often promote the construction of public transport. Unlike rail transportation or rapid transit systems, it is often difficult to estimate the vehicle arrival times at each station in a bus transportation system due to metropolitan transportation congestion. Traffic status is often monitored using wireless sensor networks (WSNs. However, WSNs are always separated from one another spatially. Recent studies have considered the connection of multiple sensor networks. This study considers a combination view of peer-to-peer (P2P overlay networks and WSN architecture to predict bus arrival times. Each bus station, which is also a P2P overlay peer, is connected in a P2P overlay network. A sensor installed in each bus can receive data via peers to obtain the moving speed of a bus. Then, each peer can exchange its data to predict bus arrival times at bus stations. This method can considerably increase the accuracy with which bus arrival times can be predicted and can provide traffic status with high precision. Furthermore, these data can also be used to plan new bus routes according to the information gathered.

  17. An interactive wireless communication system for visually impaired people using city bus transport.

    Science.gov (United States)

    Wang, Hsiao-Lan; Chen, Ya-Ping; Rau, Chi-Lun; Yu, Chung-Huang

    2014-04-25

    Visually impaired people have difficulty accessing information about public transportation systems. Several systems have been developed for assisting visually impaired and blind people to use the city bus. Most systems provide only one-way communication and require high-cost and complex equipment. The purpose of this study is to reduce the difficulties faced by visually impaired people when taking city buses, using an interactive wireless communication system. The system comprised a user module and a bus module to establish a direct one-to-one connection. When the user inputs 4-digit numbers, the user module immediately sends out the information. If the bus module receives the matched bus number, it buzzes and the warning LED flashes to notify the bus driver that someone is waiting to board on the bus. User tests were conducted by two visually impaired people in a simulated vehicle and a city bus. The success rate of interactive wireless communication, recognizing the arrival of the bus and boarding the correct bus reached 100% in all of the tests. The interactive wireless communication aid system is a valid and low-cost device for assisting visually impaired people to use city buses.

  18. Development and Evaluation of Bus Operation Control System Based on Cooperative Speed Guidance

    Directory of Open Access Journals (Sweden)

    Jing Teng

    2015-01-01

    Full Text Available Buses often have strong bunching or large interval tendency when traveling further along the route. To restrain this further deterioration of operation service, this paper developed a bus operation control system to dynamically adjust bus speed, bus dwell time, and traffic signal timings along the running path. In addition, a simulation platform was developed to evaluate the proposed control system with the actual data collected from bus route number 210 in Shanghai. The simulation results show that the proposed control system can mitigate the amplification trend of the headway deviation along the route to produce headways within a given tolerance.

  19. Alternative splicing of follicle-stimulating hormone receptor pre-mRNA: cloning and characterization of two alternatively spliced mRNA transcripts

    NARCIS (Netherlands)

    R. Kraaij (Robert); M. Verhoef-Post (Miriam); J.A. Grootegoed (Anton); A.P.N. Themmen (Axel)

    1998-01-01

    textabstractGlycoprotein hormone receptors contain a large extracellular domain that is encoded by multiple exons, facilitating the possibility of expressing alternatively spliced transcripts. We have cloned two new splice variants of the rat follicle-stimulating

  20. Androgen Receptor Splice Variants and Resistance to Taxane Chemotherapy

    Science.gov (United States)

    2017-10-01

    AWARD NUMBER: W81XWH-14-1-0480 TITLE: Androgen Receptor Splice Variants and Resistance to Taxane Chemotherapy PRINCIPAL INVESTIGATOR...Splice Variants and Resistance to Taxane Chemotherapy 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-14-1-0480 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S...During this reporting period, we have obtained approval for a no-cost extension of this award and change of research focus in the final year. This

  1. Roles of alternative splicing in modulating transcriptional regulation.

    Science.gov (United States)

    Li, Jin; Wang, Yang; Rao, Xi; Wang, Yue; Feng, Weixing; Liang, Hong; Liu, Yunlong

    2017-10-03

    The ability of a transcription factor to regulate its targets is modulated by a variety of genetic and epigenetic mechanisms. Alternative splicing can modulate gene function by adding or removing certain protein domains, and therefore affect the activity of protein. Reverse engineering of gene regulatory networks using gene expression profiles has proven valuable in dissecting the logical relationships among multiple proteins during the transcriptional regulation. However, it is unclear whether alternative splicing of certain proteins affects the activity of other transcription factors. In order to investigate the roles of alternative splicing during transcriptional regulation, we constructed a statistical model to infer whether the alternative splicing events of modulator proteins can affect the ability of key transcription factors in regulating the expression levels of their transcriptional targets. We tested our strategy in KIRC (Kidney Renal Clear Cell Carcinoma) using the RNA-seq data downloaded from TCGA (the Cancer Genomic Atlas). We identified 828of modulation relationships between the splicing levels of modulator proteins and activity levels of transcription factors. For instance, we found that the activity levels of GR (glucocorticoid receptor) protein, a key transcription factor in kidney, can be influenced by the splicing status of multiple proteins, including TP53, MDM2 (mouse double minute 2 homolog), RBM14 (RNA-binding protein 14) and SLK (STE20 like kinase). The influenced GR-targets are enriched by key cancer-related pathways, including p53 signaling pathway, TR/RXR activation, CAR/RXR activation, G1/S checkpoint regulation pathway, and G2/M DNA damage checkpoint regulation pathway. Our analysis suggests, for the first time, that exon inclusion levels of certain regulatory proteins can affect the activities of many transcription factors. Such analysis can potentially unravel a novel mechanism of how splicing variation influences the cellular

  2. A Modern Picture of Barred Galaxy Dynamics

    Science.gov (United States)

    Petersen, Michael; Weinberg, Martin; Katz, Neal

    2018-01-01

    Observations of disk galaxies suggest that bars are responsible for altering global galaxy parameters (e.g. structures, gas fraction, star formation rate). The canonical understanding of the mechanisms underpinning bar-driven secular dynamics in disk galaxies has been largely built upon the analysis of linear theory, despite galactic bars being clearly demonstrated to be nonlinear phenomena in n-body simulations. We present simulations of barred Milky Way-like galaxy models designed to elucidate nonlinear barred galaxy dynamics. We have developed two new methodologies for analyzing n-body simulations that give the best of both powerful analytic linear theory and brute force simulation analysis: orbit family identification and multicomponent torque analysis. The software will be offered publicly to the community for their own simulation analysis.The orbit classifier reveals that the details of kinematic components in galactic disks (e.g. the bar, bulge, thin disk, and thick disk components) are powerful discriminators of evolutionary paradigms (i.e. violent instabilities and secular evolution) as well as the basic parameters of the dark matter halo (mass distribution, angular momentum distribution). Multicomponent torque analysis provides a thorough accounting of the transfer of angular momentum between orbits, global patterns, and distinct components in order to better explain the underlying physics which govern the secular evolution of barred disk galaxies.Using these methodologies, we are able to identify the successes and failures of linear theory and traditional n-body simulations en route to a detailed understanding of the control bars exhibit over secular evolution in galaxies. We present explanations for observed physical and velocity structures in observations of barred galaxies alongside predictions for how structures will vary with dynamical properties from galaxy to galaxy as well as over the lifetime of a galaxy, finding that the transfer of angular

  3. First Measurement of σ(gg → t$\\bar{t}$)/σ(p$\\bar{p}$ → t$\\bar{t}$)

    Energy Technology Data Exchange (ETDEWEB)

    Alamdari, Shabnaz Pashapour [Univ. of Toronto, ON (Canada)

    2008-01-01

    The work presented here is the first measurement of the fraction of top quark pair production through gluon-gluon fusion. We use an integrated luminosity of 0.96 ± 0.06 fb-1 of p{bar p} collisions at √s of 1.96 TeV collected by the CDF II detector. We select t$\\bar{t}$ candidates by identifying a high-pT lepton candidate, a large missing ET as evidence for a neutrino candidate and at least four high ET jets, one of which has to be identified as originating from a b quark. The challenge is to discriminate between the two production processes with the identical final state, gg → t$\\bar{t}$ and q$\\bar{p}$ → t$\\bar{t}$. We take advantage of the fact that compared to a quark, a gluon is more likely to radiate a low momentum gluon and therefore, one expects a larger number of charged particles with low pT in a process involving more gluons. Given the large uncertainties associated with the modeling of the low pT charged particle multiplicity, a data-driven technique was employed. Using calibration data samples, we show there exists a clear correlation between the observed average number of low pT charged particles and the average number of gluons involved in the production process predicted by Monte Carlo calculations. Given the correlation, one can identify low pT charged particle multiplicity distributions associated with specific average number of gluons. The W + 0 jet sample and dijets sample with leading jet ET in the range of 80-100 GeV are used to find no-gluon and gluon-rich low p{sub T} charged particle multiplicity distributions, respectively. Using these no-gluon and gluon-rich distributions in a likelihood fit, we find the fraction of gluon-rich events in t{bar t} candidates. This fraction has contributions from the signal and background events. Taking into account these contributions and the gg → t$\\bar{t}$ and q$\\bar{q}$ → t$\\bar

  4. RNA splicing is responsive to MBNL1 dose.

    Directory of Open Access Journals (Sweden)

    Sonali P Jog

    Full Text Available Myotonic dystrophy (DM1 is a highly variable, multi-system disorder resulting from the expansion of an untranslated CTG tract in DMPK. In DM1 expanded CUG repeat RNAs form hairpin secondary structures that bind and aberrantly sequester the RNA splice regulator, MBNL1. RNA splice defects resulting as a consequence of MBNL1 depletion have been shown to play a key role in the development of DM1 pathology. In patient populations, both the number and severity of DM1 symptoms increase broadly as a function of CTG tract length. However significant variability in the DM1 phenotype is observed in patients encoding similar CTG repeat numbers. Here we demonstrate that a gradual decrease in MBNL1 levels results both in the expansion of the repertoire of splice defects and an increase in the severity of the splice alterations. Thus, MBNL1 loss does not have an all or none outcome but rather shows a graded effect on the number and severity of the ensuing splice defects. Our results suggest that once a critical threshold is reached, relatively small dose variations of free MBNL1 levels, which may reflect modest changes in the size of the CUG tract or the extent of hairpin secondary structure formation, can significantly alter the number and severity of splice abnormalities and thus contribute to the phenotype variability observed in DM1 patients.

  5. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    Directory of Open Access Journals (Sweden)

    Zodwa Dlamini

    2015-11-01

    Full Text Available Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 family have alternatively spliced isoforms that lack important active domains. These isoforms can play a negative regulatory role by binding to and inhibiting the pro-apoptotic forms. Alternative splicing is observed to be increased in various cardiovascular diseases with the level of alternate transcripts increasing elevated in diseased hearts compared to healthy subjects. In many cases these isoforms appear to be the underlying cause of the disease, while in others they may be induced in response to cardiovascular pathologies. Regardless of this, the detection of alternate splicing events in the heart can serve as useful diagnostic or prognostic tools, while those splicing events that seem to play a causative role in cardiovascular disease make attractive future drug targets.

  6. Splice site mutations in the ATP7A gene.

    Directory of Open Access Journals (Sweden)

    Tina Skjørringe

    Full Text Available Menkes disease (MD is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12 mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation to in vivo results. Ninety-six percent of the mutations identified in 45 patients with classical MD were predicted to have a significant effect on splicing, which concurs with the absence of any detectable wild-type transcript in all 19 patients investigated in vivo. Sixty-seven percent of the mutations identified in 12 patients with milder phenotypes were predicted to have no significant effect on splicing, which concurs with the presence of wild-type transcript in 7 out of 9 patients investigated in vivo. Both the in silico predictions and the in vivo results support the hypothesis previously suggested by us and others, that the presence of some wild-type transcript is correlated to a milder phenotype.

  7. Width of gene expression profile drives alternative splicing.

    Directory of Open Access Journals (Sweden)

    Daniel Wegmann

    Full Text Available Alternative splicing generates an enormous amount of functional and proteomic diversity in metazoan organisms. This process is probably central to the macromolecular and cellular complexity of higher eukaryotes. While most studies have focused on the molecular mechanism triggering and controlling alternative splicing, as well as on its incidence in different species, its maintenance and evolution within populations has been little investigated. Here, we propose to address these questions by comparing the structural characteristics as well as the functional and transcriptional profiles of genes with monomorphic or polymorphic splicing, referred to as MS and PS genes, respectively. We find that MS and PS genes differ particularly in the number of tissues and cell types where they are expressed.We find a striking deficit of PS genes on the sex chromosomes, particularly on the Y chromosome where it is shown not to be due to the observed lower breadth of expression of genes on that chromosome. The development of a simple model of evolution of cis-regulated alternative splicing leads to predictions in agreement with these observations. It further predicts the conditions for the emergence and the maintenance of cis-regulated alternative splicing, which are both favored by the tissue specific expression of splicing variants. We finally propose that the width of the gene expression profile is an essential factor for the acquisition of new transcript isoforms that could later be maintained by a new form of balancing selection.

  8. Digital holographic microtomography of fusion spliced optical fibers

    Science.gov (United States)

    Deng, Yating; Xiao, Wen; Ma, Xichao; Pan, Feng

    2017-03-01

    In this paper, we report three-dimensional(3D) measurement results of structural parameters of fusion spliced optical fibers using digital holographic microtomography. A holographic setup in microscopy configuration with the sample-fixed and setup-rotating scheme is established. A series of holograms is recorded from various incident angles. Then the filtered backprojection algorithm is applied to reconstruct the 3D refractive index (RI) distributions of the fusion spliced optical fibers inserted in the index-matching liquid. Experimental results exhibit the internal and external shapes of three kinds of fusion splices between different fibers, including a single-mode fiber(SMF) and a multimode fiber, an SMF and a panda polarization maintaining fiber (Panda PMF), and an SMF and a bow-tie polarization maintaining fiber (Bow-Tie PMF). With 3D maps of RI, it is intuitive to observe internal structural details of fused fibers and evaluate the splicing quality. This paper describes a powerful method for non-invasive microscopic measurement of fiber splicing. Furthermore, it provides the possibility of detecting fiber splicing loss by 3D structures.

  9. Massachusetts Fuel Cell Bus Project: Demonstrating a Total Transit Solution for Fuel Cell Electric Buses in Boston

    Energy Technology Data Exchange (ETDEWEB)

    2017-05-22

    The Federal Transit Administration's National Fuel Cell Bus Program focuses on developing commercially viable fuel cell bus technologies. Nuvera is leading the Massachusetts Fuel Cell Bus project to demonstrate a complete transit solution for fuel cell electric buses that includes one bus and an on-site hydrogen generation station for the Massachusetts Bay Transportation Authority (MBTA). A team consisting of ElDorado National, BAE Systems, and Ballard Power Systems built the fuel cell electric bus, and Nuvera is providing its PowerTap on-site hydrogen generator to provide fuel for the bus.

  10. What Makes the Family of Barred Disc Galaxies So Rich: Damping Stellar Bars in Spinning Haloes

    Science.gov (United States)

    Collier, Angela; Shlosman, Isaac; Heller, Clayton

    2018-02-01

    We model and analyze the secular evolution of stellar bars in spinning dark matter (DM) haloes with the cosmological spin λ ˜ 0 - 0.09. Using high-resolution stellar and DM numerical simulations, we focus on angular momentum exchange between stellar discs and DM haloes of various axisymmetric shapes — spherical, oblate and prolate. We find that stellar bars experience a diverse evolution which is guided by the ability of parent haloes to absorb angular momentum, J, lost by the disc through the action of gravitational torques, resonant and non-resonant. We confirm that dynamical bar instability is accelerated via resonant J-transfer to the halo. Our main findings relate to the long-term, secular evolution of disc-halo systems: with an increasing λ, bars experience less growth and basically dissolve after they pass through vertical buckling instability. Specifically, with increasing λ, (1) The vertical buckling instability in stellar bars colludes with inability of the inner halo to absorb J — this emerges as the main factor weakening or destroying bars in spinning haloes; (2) Bars lose progressively less J, and their pattern speeds level off; (3) Bars are smaller, and for λ ≳ 0.06 cease their growth completely following buckling; (4) Bars in λ > 0.03 halos have ratio of corotation-to-bar radii, RCR/Rb > 2, and represent so-called slow bars without offset dust lanes. We provide a quantitative analysis of J-transfer in disc-halo systems, and explain the reasons for absence of growth in fast spinning haloes and its observational corollaries. We conclude that stellar bar evolution is substantially more complex than anticipated, and bars are not as resilient as has been considered so far.

  11. Physics Bus: An Innovative Model for Public Engagement

    Science.gov (United States)

    Fox, Claire

    The Physics Bus is about doing science for fun. It is an innovative model for science outreach whose mission is to awaken joy and excitement in physics for all ages and walks of life - especially those underserved by science enrichment. It is a mobile exhibition of upcycled appliances-reimagined by kids-that showcase captivating physics phenomena. Inside our spaceship-themed school bus, visitors will find: a microwave ionized-gas disco-party, fog rings that shoot from a wheelbarrow tire, a tv whose electron beam is controlled by a toy keyboard, and over 20 other themed exhibits. The Physics Bus serves a wide range of public in diverse locations from local neighborhoods, urban parks and rural schools, to cross-country destinations. Its approachable, friendly and relaxed environment allows for self-paced and self-directed interactions, providing a positive and engaging experience with science. We believe that this environment enriches lives and inspires people. In this presentation we will talk about the nuts and bolts that make this model work, how the project got started, and the resources that keep it going. We will talk about the advantages of being a grassroots and community-based organization, and how programs like this can best interface with universities. We will explain the benefits of focusing on direct interactions and why our model avoids ``teaching'' physics content with words. Situating our approach within a body of research on the value of informal science we will discuss our success in capturing and engaging our audience. By the end of this presentation we hope to broaden your perception of what makes a successful outreach program and encourage you to value and support alternative outreach models such as this one. In Collaboration with: Eva Luna, Cornell University; Erik Herman, Cornell University; Christopher Bell, Ithaca City School District.

  12. Exonic splicing enhancer-dependent selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site can be rescued in a cell lacking splicing factor ASF/SF2 through activation of the phosphatidylinositol 3-kinase/Akt pathway.

    Science.gov (United States)

    Liu, Xuefeng; Mayeda, Akila; Tao, Mingfang; Zheng, Zhi-Ming

    2003-02-01

    Bovine papillomavirus type 1 (BPV-1) late pre-mRNAs are spliced in keratinocytes in a differentiation-specific manner: the late leader 5' splice site alternatively splices to a proximal 3' splice site (at nucleotide 3225) to express L2 or to a distal 3' splice site (at nucleotide 3605) to express L1. Two exonic splicing enhancers, each containing two ASF/SF2 (alternative splicing factor/splicing factor 2) binding sites, are located between the two 3' splice sites and have been identified as regulating alternative 3' splice site usage. The present report demonstrates for the first time that ASF/SF2 is required under physiological conditions for the expression of BPV-1 late RNAs and for selection of the proximal 3' splice site for BPV-1 RNA splicing in DT40-ASF cells, a genetically engineered chicken B-cell line that expresses only human ASF/SF2 controlled by a tetracycline-repressible promoter. Depletion of ASF/SF2 from the cells by tetracycline greatly decreased viral RNA expression and RNA splicing at the proximal 3' splice site while increasing use of the distal 3' splice site in the remaining viral RNAs. Activation of cells lacking ASF/SF2 through anti-immunoglobulin M-B-cell receptor cross-linking rescued viral RNA expression and splicing at the proximal 3' splice site and enhanced Akt phosphorylation and expression of the phosphorylated serine/arginine-rich (SR) proteins SRp30s (especially SC35) and SRp40. Treatment with wortmannin, a specific phosphatidylinositol 3-kinase/Akt kinase inhibitor, completely blocked the activation-induced activities. ASF/SF2 thus plays an important role in viral RNA expression and splicing at the proximal 3' splice site, but activation-rescued viral RNA expression and splicing in ASF/SF2-depleted cells is mediated through the phosphatidylinositol 3-kinase/Akt pathway and is associated with the enhanced expression of other SR proteins.

  13. Genomic and Transcriptomic Analysis Reveals Spliced Leader Trans-Splicing in Cryptomonads.

    Science.gov (United States)

    Roy, Scott William

    2017-03-01

    Spliced leader trans-splicing (SLTS) is a poorly understood mechanism that is found in a diversity of eukaryotic lineages. In SLTS, a short RNA sequence is added near the 5' ends of the transcripts of protein-coding genes by a modified spliceosomal reaction. Available data suggest that SLTS has evolved many times, and might be more likely to evolve in animals. That SLTS might be more likely to evolve in the context of the generally complex transcriptomes characteristic of animals suggests the possibility that SLTS functions in gene regulation or transcriptome diversification, however no general novel function for SLTS is known. Here, I report SLTS in a lineage of cellularly complex unicellular eukaryotes. Cryptomonads are a group of eukaryotic algae that acquired photosynthetic capacity by secondary endosymbiosis of a red alga, and that retain a reduced copy of the nucleus of the engulfed alga. I estimate that at least one-fifth of genes in the model cryptomonad Guillardia theta and its relative Hanusia phi undergo SLTS. I show that hundreds of genes in G. theta generate alternative transcripts by SLTS at alternative sites, however I find little evidence for alternative protein production by alternative SLTS splicing. Interestingly, I find no evidence for substantial operon structure in the G. theta genome, in contrast to previous findings in other lineages with SLTS. These results extend SLTS to another major group of eukaryotes, and heighten the mystery of the evolution of SLTS and its association with cellular and transcriptomic complexity. © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  14. Human splicing factor ASF/SF2 encodes for a repressor domain required for its inhibitory activity on pre-mRNA splicing.

    Science.gov (United States)

    Dauksaite, Vita; Akusjärvi, Göran

    2002-04-12

    The essential splicing factor ASF/SF2 activates or represses splicing depending on where on the pre-mRNA it binds. We have shown previously that ASF/SF2 inhibits adenovirus IIIa pre-mRNA splicing by binding to an intronic repressor element. Here we used MS2-ASF/SF2 fusion proteins to show that the second RNA binding domain (RBD2) is both necessary and sufficient for the splicing repressor function of ASF/SF2. Furthermore, we show that the completely conserved SWQDLKD motif in ASF/SF2-RBD2 is essential for splicing repression. Importantly, this heptapeptide motif is unlikely to be directly involved in RNA binding given its position within the predicted structure of RBD2. The activity of the ASF/SF2-RBD2 domain in splicing was position-dependent. Thus, tethering RBD2 to the IIIa intron resulted in splicing repression, whereas RBD2 binding at the second exon had no effect on IIIa splicing. The splicing repressor activity of RBD2 was not unique to the IIIa pre-mRNA, as binding of RBD2 at an intronic position in the rabbit beta-globin pre-mRNA also resulted in splicing inhibition. Taken together, our results suggest that ASF/SF2 encode distinct domains responsible for its function as a splicing enhancer or splicing repressor protein.

  15. BC Transit Fuel Cell Bus Project Evaluation Results: Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Post, M.

    2014-09-01

    Second report evaluating a fuel cell electric bus (FCEB) demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. NREL published its first report on the demonstration in February 2014. This report is an update to the previous report; it covers 3 full years of revenue service data on the buses from April 2011 through March 2014 and focuses on the final experiences and lessons learned.

  16. Implementation of PMSM speed control software based on CAN bus

    Science.gov (United States)

    Cao, Wenlun; Chen, Bei; He, Yuyao

    2013-03-01

    In this paper, the driver's hardware structure based on TMS320F28335 is introduced, the control software flow of host computer based on CAN bus is designed, the rule of CAN communication protocol is fulfilled and accordingly the hybrid programming is realized in the background of low speed and large sinusoid operation. This system can realize the CAN communication setting, download the PID parameters to DSP, operate at constant rotate speed and at given large sinusoid rotate speed. Meanwhile the dynamical monitoring and alarm are implemented. Finally the real-time display and storage of measured current, voltage and rotate speed are completed well.

  17. Expression of alternatively spliced sodium channel alpha-subunit genes. Unique splicing patterns are observed in dorsal root ganglia.

    Science.gov (United States)

    Raymond, Christopher K; Castle, John; Garrett-Engele, Philip; Armour, Christopher D; Kan, Zhengyan; Tsinoremas, Nicholas; Johnson, Jason M

    2004-10-29

    Molecular medicine requires the precise definition of drug targets, and tools are now in place to provide genome-wide information on the expression and alternative splicing patterns of any known gene. DNA microarrays were used to monitor transcript levels of the nine well-characterized alpha-subunit sodium channel genes across a broad range of tissues from cynomolgus monkey, a non-human primate model. Alternative splicing of human transcripts for a subset of the genes that are expressed in dorsal root ganglia, SCN8A (Na(v)1.6), SCN9A (Na(v)1.7), and SCN11A (Na(v)1.9) was characterized in detail. Genomic sequence analysis among gene family paralogs and between cross-species orthologs suggested specific alternative splicing events within transcripts of these genes, all of which were experimentally confirmed in human tissues. Quantitative PCR revealed that certain alternative splice events are uniquely expressed in dorsal root ganglia. In addition to characterization of human transcripts, alternatively spliced sodium channel transcripts were monitored in a rat model for neuropathic pain. Consistent down-regulation of all transcripts was observed, as well as significant changes in the splicing patterns of SCN8A and SCN9A.

  18. Biochemical identification of new proteins involved in splicing repression at the Drosophila P-element exonic splicing silencer

    Science.gov (United States)

    Horan, Lucas; Yasuhara, Jiro C.; Kohlstaedt, Lori A.; Rio, Donald C.

    2015-01-01

    Splicing of the Drosophila P-element third intron (IVS3) is repressed in somatic tissues due to the function of an exonic splicing silencer (ESS) complex present on the 5′ exon RNA. To comprehensively characterize the mechanisms of this alternative splicing regulation, we used biochemical fractionation and affinity purification to isolate the silencer complex assembled in vitro and identify the constituent proteins by mass spectrometry. Functional assays using splicing reporter minigenes identified the proteins hrp36 and hrp38 and the cytoplasmic poly(A)-binding protein PABPC1 as novel functional components of the splicing silencer. hrp48, PSI, and PABPC1 have high-affinity RNA-binding sites on the P-element IVS3 5′ exon, whereas hrp36 and hrp38 proteins bind with low affinity to the P-element silencer RNA. RNA pull-down and immobilized protein assays showed that hrp48 protein binding to the silencer RNA can recruit hrp36 and hrp38. These studies identified additional components that function at the P-element ESS and indicated that proteins with low-affinity RNA-binding sites can be recruited in a functional manner through interactions with a protein bound to RNA at a high-affinity binding site. These studies have implications for the role of heterogeneous nuclear ribonucleoproteins (hnRNPs) in the control of alternative splicing at cis-acting regulatory sites. PMID:26545814

  19. Magnetorheological stabilizer bar for ground vehicles

    Science.gov (United States)

    Bai, Xian-Xu; Xu, Shi-Xu; Shen, Sheng

    2017-04-01

    Aim at simultaneously improving the safety (anti-roll performance) and ride comfort of vehicles during fast cornering or over road irregularities, the principle and configuration of magnetorheological (MR) semi-active stabilizer bar is proposed in this paper. The MR stabilizer bar featuring a rotary MR damper is used to provide small torsional torque at low speed to improve ride comfort, while large torsional torque to enhance the safety at high speed cornering. To verify the feasibility and effectiveness of the proposed MR stabilizer bar, the mathematical model of the dynamic system is established, and the passive-on control performance of a specific full vehicle is studied via a dynamics simulation software ADAMS, and the performance is compared with the conventional passive stabilizer bar for ground vehicle dynamic system.

  20. Raising the Bar for Microbiologists | IDRC - International ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2010-12-09

    Dec 9, 2010 ... SEE ALSO... Audio: Raising the bar for Microbiologists Professor Ana Sanchez and Professor Lourdes Enriquez explain why zoonotic diseases, transfered from animals to humans, require multidisciplinary, integrated solutions.

  1. Barring intervention? Lesbian and gay bars as an underutilized venue for tobacco interventions.

    Science.gov (United States)

    Leibel, Katherine; Lee, Joseph G L; Goldstein, Adam O; Ranney, Leah M

    2011-07-01

    Lesbian, gay, bisexual, and transgender (LGBT) communities are at high risk for tobacco use. While LGBT communities have historically considered bars to be safe places to socialize and congregate, these spaces are often tobacco-friendly environments and may have potential as sites for much needed intervention. Only a few public health interventions have attempted to work through bars and clubs to decrease tobacco use in the LGBT populations. Evidence from HIV prevention suggests some potential interventions in bars, and the tobacco industry has worked extensively (and successfully) to utilize bars in marketing efforts. Lesbian and gay bars are underutilized in tobacco control, suggesting missed avenues for chronic disease prevention programs. Researchers and communities should continue to recognize the importance of clean indoor air laws covering bars and develop additional strategies for reaching LGBT populations with disparities.

  2. Intelligent Bar Chart Plagiarism Detection in Documents

    Directory of Open Access Journals (Sweden)

    Mohammed Mumtaz Al-Dabbagh

    2014-01-01

    Full Text Available This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR. By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts.

  3. Intelligent Bar Chart Plagiarism Detection in Documents

    Science.gov (United States)

    Al-Dabbagh, Mohammed Mumtaz; Salim, Naomie; Alkawaz, Mohammed Hazim; Saba, Tanzila; Al-Rodhaan, Mznah; Al-Dhelaan, Abdullah

    2014-01-01

    This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR). By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts. PMID:25309952

  4. Chocolate Bars Based on Human Nutritional Requirements

    OpenAIRE

    Robson, Anthony,

    2013-01-01

    International audience; Key Points * The nutritional value of chocolate bars should be based on the nutritional value of the low energy dense late Paleolithic human diet to help reduce mental ill health, obesity, and other postprandial insults. * Current chocolate bars have a high energy density (>2 kcal/g). * Cocoa can be sweetened by the addition of calorie-free Purefruit™ (Tate & Lyle) monk fruit ( Siraitia grosvenorii ) extract. PUREFRUIT™ is approximately 200 times sweeter than sugar and...

  5. Intelligent bar chart plagiarism detection in documents.

    Science.gov (United States)

    Al-Dabbagh, Mohammed Mumtaz; Salim, Naomie; Rehman, Amjad; Alkawaz, Mohammed Hazim; Saba, Tanzila; Al-Rodhaan, Mznah; Al-Dhelaan, Abdullah

    2014-01-01

    This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR). By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts.

  6. Delamination stresses in semicircular laminated composite bars

    Science.gov (United States)

    Ko, William L.

    1988-01-01

    Using anisotropic elasticity theory, delamination stresses in a semicircular laminated composite curved bar subjected to end forces and end moments were calculated, and their radial locations determined. A family of design curves was presented, showing variation of the intensity of delamination stresses and their radial locations with different geometry and different degrees of anisotropy of the curved bar. The effect of anisotropy on the location of peak delamination stress was found to be small.

  7. Companions of Bright Barred Shapley Ames Galaxies

    OpenAIRE

    Garcia-Barreto, J. Antonio; Carrillo, Rene; Vera-Villamizar, Nelson

    2003-01-01

    Companion galaxy environment for a subset of 78 bright and nearby barred galaxies from the Shapley Ames Catalog is presented. Among spiral barred galaxies there are Seyfert galaxies, galaxies with circumnuclear structures, galaxies not associated with any large scale galaxy cloud structure, galaxies with peculiar disk morphology (crooked arms) and galaxies with normal disk morphology; the list includes all Hubble types. The companion galaxy list includes number of companion galaxies within 20...

  8. Preparation of a Breadfruit Flour Bar

    Science.gov (United States)

    Nochera, Carmen L.; Ragone, Diane

    2016-01-01

    Breadfruit is a nutritious, high energy food with a low quantity of protein but excellent protein quality. It has the potential to be developed into desired products which will help increase its utilization and add value to the crop. The overall purposes of this investigation were to develop a portable, nutritious, ready-to-eat breadfruit product (bar), test the sensory qualities of the product, and evaluate the nutritional properties of the product. Flour made from the Micronesian variety, Meinpadahk (Artocarpus altilis × Artocarpus mariannensis), was utilized for the development of the breadfruit bar. Breadfruit is a rich source of fiber, vitamins such as vitamin C, minerals such as potassium, and phytochemicals such as flavonoids. Nutritional labeling indicates that the breadfruit bar is high in carbohydrates and low in fat, and sensory evaluation indicates that 81% of the panelists found the bar acceptable while 19% disliked the bar. The breadfruit bar can provide an appealing and inexpensive gluten-free food source based on locally available breadfruit. PMID:28231132

  9. Optimized surgical tool for pectus bar extraction.

    Science.gov (United States)

    Rechowicz, Krzysztof J; McKenzie, Frederic D; Bawab, Sebastian Y; Obermeyer, Robert

    2010-01-01

    Surgeons on a daily basis improve or rescue human lives. Therefore, they should be provided with the most optimal tools so their skills are fully utilized. In this paper, we present such an optimized tool for surgeons who employ the Nuss procedure to correct pectus excavatum - a congenital chest wall deformity. The Nuss procedure is a minimally invasive procedure that results in the placement of a metal bar inside the chest cavity. The bar is removed after approximately two years. Surgeons have been reporting that the currently available tools for the bar extraction do not provide the most optimal functionality. Therefore, we have proposed an optimized and improved design of the tool for the bar extraction. The improved design tool is further analyzed using finite element techniques. Additionally, we have built a physical prototype to ensure that the new tool to seamlessly integrate with the bar and to further evaluate by the surgeons who routinely practice the Nuss procedure. In order to validate in the future the final design, we have manufactured wax models that will serve as the patterns in the casting process of metal prototypes. They should provide enough strength to withstand stresses present in the bar straightening process.

  10. Boxy Orbital Structures in Rotating Bar Models

    Science.gov (United States)

    Chaves-Velasquez, L.; Patsis, P. A.; Puerari, I.; Skokos, Ch.; Manos, T.

    2017-12-01

    We investigate regular and chaotic two-dimensional (2D) and three-dimensional (3D) orbits of stars in models of a galactic potential consisting of a disk, a halo, and a bar to find the origin of boxy components that are part of the bar or (almost) the bar itself. Our models originate in snapshots of an N-body simulation, which develops a strong bar. We consider three snapshots of the simulation, and, for the orbital study, we treat each snapshot independently, as an autonomous Hamiltonian system. The calculated corotation–to–bar length ratios indicate that in all three cases, the bar rotates slowly, while the orientation of the orbits of the main family of periodic orbits changes along its characteristic. We characterize the orbits as regular, sticky, or chaotic after integrating them for a 10 Gyr period by using the GALI2 index. Boxiness in the equatorial plane is associated either with quasi-periodic orbits in the outer parts of stability islands or with sticky orbits around them, which can be found in a large range of energies. We indicate the location of such orbits in diagrams, which include the characteristic of the main family. They are always found about the transition region from order to chaos. By perturbing such orbits in the vertical direction, we find a class of 3D nonperiodic orbits, which have boxy projections both in their face-on and side-on views.

  11. $\\bar d - \\bar u$ asymmetry in the proton in chiral effective theory

    Energy Technology Data Exchange (ETDEWEB)

    Salamu, Yusupujiang [Institute of High Energy Physics, CAS, Beijing (China); Ji, Chueng -Ryong [North Carolina State Univ., Raleigh, NC (United States); Melnitchouk, W. [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Wang, P. [Institute of High Energy Physics, Beijing (China); Theoretical Physics Center for Science Facilities, CAS, Beijing (China)

    2015-03-25

    We compute the $\\bar d - \\bar u$ asymmetry in the proton in chiral effective theory, including both nucleon and Δ degrees of freedom, within both relativistic and heavy baryon frameworks. In addition to the distribution at $x>0$, we estimate the correction to the integrated asymmetry arising from zero momentum contributions from pion rainbow and bubble diagrams at $x=0$, which have not been accounted for in previous analyses. In conclusion, we find that the empirical $x$ dependence of $\\bar d - \\bar u$ as well as the integrated asymmetry can be well reproduced in terms of a transverse momentum cutoff parameter.

  12. Impact of Efficient Driving in Professional Bus Fleets

    Directory of Open Access Journals (Sweden)

    Roberto Garcia

    2017-12-01

    Full Text Available Diesel engines of the vehicles in the transport sector are responsible for most of the CO2 emissions into the environment. An alternative to reduce fuel consumption is to promote efficient driving techniques. The aim of this paper is to assess the impact of efficient driving on fuel consumption in professional fleets. Data captured from the engine control unit (ECU of the vehicles are complemented with external information from weather stations and context data from the transport companies. The paper proposes linear and quadratic models in order to quantify the impact of all the terms influencing energy consumption. The analysis was made from the traces captured from a passenger transport company in Spain with more than 50 bus routes. 20 vehicles of five different models were monitored and 58 drivers participated in the study. The results indicate that efficient driving has significant influence on fuel consumption, which confirms efficient driving as a valid and economical option for reducing consumption and therefore emissions of harmful particles into the atmosphere. According to the proposed model, in average external conditions, a driver that increases efficiency from 25% to 75% can reach savings in fuel consumption of up to 16 L/100 km in the analysed bus fleet, which is a significant improvement considering the long distances covered by professionals of the transport sector.

  13. King County Metro Battery Electric Bus Demonstration: Preliminary Project Results

    Energy Technology Data Exchange (ETDEWEB)

    2017-05-22

    The U.S. Federal Transit Administration (FTA) funds a variety of research projects that support the commercialization of zero-emission bus technology. To evaluate projects funded through these programs, FTA has enlisted the help of the National Renewable Energy Laboratory (NREL) to conduct third-party evaluations of the technologies deployed under the FTA programs. NREL works with the selected agencies to evaluate the performance of the zero-emission buses compared to baseline conventional buses in similar service. The evaluation effort will advance the knowledge base of zero-emission technologies in transit bus applications and provide 'lessons learned' to aid other fleets in incrementally introducing next generation zero-emission buses into their operations. This report provides preliminary performance evaluation results from a demonstration of three zero-emission battery electric buses at King County Metro in King County, Washington. NREL developed this preliminary results report to quickly disseminate evaluation results to stakeholders. Detailed evaluation results will be published in future reports.

  14. Operational efficiency of the lighting system of bus salons

    Directory of Open Access Journals (Sweden)

    Brytkovskyi V.M.

    2016-08-01

    Full Text Available In recent years the problem of safety of people is studied primarily in aspects of natural and man-made disasters, fire, health and safety in the workplace. A problem connected with the way of life of the people, in particular with the use of bus transport remains almost out of sight. In addition to the foregoing, there is another side to the issue: modern development of industry and transport is characterized by large-scale introduction of technical measures aimed at saving energy In the specified aspect theoretical dependences for evaluation of technological economic efficiency of light sources in indoor lighting system buses are grounded. This theoretical dependency will make analytical framework justification applying the respective sources of light, taking into account the hygiene requirements to illumination. The methodology of calculation of economic efficiency of lighting of bus salons is offered. Estimating parameter is justified relative objective function value costs per unit of time or distance. The greatest costs are typical for lamps with incandescent bulbs. The least is common to lamps with fluorescent lamps. Led bulbs have no significant advantage even over incandescent lamps. The reason for this is the relatively high color temperature radiation of LED lamps that requires more light levels and, consequently, the high cost of energy, as well as relatively large initial cost of these lamps.

  15. The school bus routing and scheduling problem with transfers.

    Science.gov (United States)

    Bögl, Michael; Doerner, Karl F; Parragh, Sophie N

    2015-03-01

    In this article, we study the school bus routing and scheduling problem with transfers arising in the field of nonperiodic public transportation systems. It deals with the transportation of pupils from home to their school in the morning taking the possibility that pupils may change buses into account. Allowing transfers has several consequences. On the one hand, it allows more flexibility in the bus network structure and can, therefore, help to reduce operating costs. On the other hand, transfers have an impact on the service level: the perceived service quality is lower due to the existence of transfers; however, at the same time, user ride times may be reduced and, thus, transfers may also have a positive impact on service quality. The main objective is the minimization of the total operating costs. We develop a heuristic solution framework to solve this problem and compare it with two solution concepts that do not consider transfers. The impact of transfers on the service level in terms of time loss (or user ride time) and the number of transfers is analyzed. Our results show that allowing transfers reduces total operating costs significantly while average and maximum user ride times are comparable to solutions without transfers. © 2015 Wiley Periodicals, Inc. NETWORKS, Vol. 65(2), 180-203 2015.

  16. CERPEN FARUK “BUS KOTA” DALAM SEMIOTIK ROLAND BARTHES

    Directory of Open Access Journals (Sweden)

    Ken Widyatwati

    2016-04-01

    Full Text Available Literature called beautiful if it contains the mandate, message, ideas and meanings that are useful for the readers. Short story “Bus Kota” (City Bus containing the mandate, message, ideas and meanings that are useful to the reader. The mandate will be revealed by Roland Barthes' semiotic theory in the book S/Z. Literature has its own allure and text have accrued but not due to independence because of the difference in quality. Texts merge into a unity, or restore any text not as an individual but as a form of plurality. Literature as part of a social system is an expression of the author's sense of the outpouring of public life. Literary works can express themselves in the author's view the social and cultural changes in society because of the development of information technology and rapid. Technological developments and socio-cultural information can change in society so as to affect the psychology of the people. Society must have a thickness of faith not only for themselves, but to be able to maintain equanimity in the life of a pluralistic society

  17. Bus Implementation Using New Low Power PFSCL Tristate Buffers

    Directory of Open Access Journals (Sweden)

    Neeta Pandey

    2016-01-01

    Full Text Available This paper proposes new positive feedback source coupled logic (PFSCL tristate buffers suited to bus applications. The proposed buffers use switch to attain high impedance state and modify the load or the current source section. An interesting consequence of this is overall reduction in the power consumption. The proposed tristate buffers consume half the power compared to the available switch based counterpart. The issues with available PFSCL tristate buffers based bus implementation are identified and benefits of employing the proposed tristate buffer topologies are put forward. SPICE simulation results using TSMC 180 nm CMOS technology parameters are included to support the theoretical formulations. The performance of proposed tristate buffer topologies is examined on the basis of propagation delay, output enable time, and power consumption. It is found that one of the proposed tristate buffer topology outperforms the others in terms of all the performance parameters. An examination of behavior of available and the proposed PFSCL tristate buffer topologies under parameter variations and mismatch shows a maximum variation of 14%.

  18. Studying customer loyalty at Daewoo express bus service, Pakistan

    Directory of Open Access Journals (Sweden)

    Usman Yousaf

    2013-09-01

    Full Text Available Background: Meeting service quality standards and striving for loyalty are two critical areas which have until now been overlooked by both the passenger transport industry and academia in Pakistan therefore the study sheds useful light on an issue hitherto untouched. Therefore increased sample size will help in increasing generalizability of study. Methods: This paper is designed to study the level of loyalty at Daewoo Express Bus Service in Sargodha by measuring customer satisfaction. Researchers used a small sample of only 96 respondents (passengers and only studies customer's behavior in the service quality of Daewoo Express Bus Service however it may differ in other passenger transport services. Results and conclusions: This paper reveals that there is a positive and significant relationship between loyalty (dependant variable and price perceptions, reliability, retrieving and smoothing (independent variables. However, the study found that reliability of services is the most important dimension among other independent variables (price perceptions, smoothing, reliability, retrieving effecting customers loyalty at Daewoo Express. Paper under consideration would surely assist Daewoo`s management team to take care of the loopholes existing in the current service level and likely threats which Daewoo might face.

  19. Efficiency and robustness of different bus network designs

    Science.gov (United States)

    Pang, John Zhen Fu; Bin Othman, Nasri; Ng, Keng Meng; Monterola, Christopher

    2015-07-01

    We compare the efficiencies and robustness of four transport networks that can be possibly formed as a result of deliberate city planning. The networks are constructed based on their spatial resemblance to the cities of Manhattan (lattice), Sudan (random), Beijing (single-blob) and Greater Cairo (dual-blob). For a given type, a genetic algorithm is employed to obtain an optimized set of the bus routes. We then simulate how commuter travels using Yen's algorithms for k shortest paths on an adjacency matrix. The cost of traveling such as walking between stations is captured by varying the weighted sums of matrices. We also consider the number of transfers a posteriori by looking at the computed shortest paths. With consideration to distances via radius of gyration, redundancies of travel and number of bus transfers, our simulations indicate that random and dual-blob are more efficient than single-blob and lattice networks. Moreover, dual-blob type is least robust when node removals are targeted but is most resilient when node failures are random. The work hopes to guide and provide technical perspectives on how geospatial distribution of a city limits the optimality of transport designs.

  20. Regulation of plant developmental processes by a novel splicing factor.

    Directory of Open Access Journals (Sweden)

    Gul Shad Ali

    Full Text Available Serine/arginine-rich (SR proteins play important roles in constitutive and alternative splicing and other aspects of mRNA metabolism. We have previously isolated a unique plant SR protein (SR45 with atypical domain organization. However, the biological and molecular functions of this novel SR protein are not known. Here, we report biological and molecular functions of this protein. Using an in vitro splicing complementation assay, we showed that SR45 functions as an essential splicing factor. Furthermore, the alternative splicing pattern of transcripts of several other SR genes was altered in a mutant, sr45-1, suggesting that the observed phenotypic abnormalities in sr45-1 are likely due to altered levels of SR protein isoforms, which in turn modulate splicing of other pre-mRNAs. sr45-1 exhibited developmental abnormalities, including delayed flowering, narrow leaves and altered number of petals and stamens. The late flowering phenotype was observed under both long days and short days and was rescued by vernalization. FLC, a key flowering repressor, is up-regulated in sr45-1 demonstrating that SR45 influences the autonomous flowering pathway. Changes in the alternative splicing of SR genes and the phenotypic defects in the mutant were rescued by SR45 cDNA, further confirming that the observed defects in the mutant are due to the lack of SR45. These results indicate that SR45 is a novel plant-specific splicing factor that plays a crucial role in regulating developmental processes.

  1. Method of predicting Splice Sites based on signal interactions

    Directory of Open Access Journals (Sweden)

    Deogun Jitender S

    2006-04-01

    Full Text Available Abstract Background Predicting and proper ranking of canonical splice sites (SSs is a challenging problem in bioinformatics and machine learning communities. Any progress in SSs recognition will lead to better understanding of splicing mechanism. We introduce several new approaches of combining a priori knowledge for improved SS detection. First, we design our new Bayesian SS sensor based on oligonucleotide counting. To further enhance prediction quality, we applied our new de novo motif detection tool MHMMotif to intronic ends and exons. We combine elements found with sensor information using Naive Bayesian Network, as implemented in our new tool SpliceScan. Results According to our tests, the Bayesian sensor outperforms the contemporary Maximum Entropy sensor for 5' SS detection. We report a number of putative Exonic (ESE and Intronic (ISE Splicing Enhancers found by MHMMotif tool. T-test statistics on mouse/rat intronic alignments indicates, that detected elements are on average more conserved as compared to other oligos, which supports our assumption of their functional importance. The tool has been shown to outperform the SpliceView, GeneSplicer, NNSplice, Genio and NetUTR tools for the test set of human genes. SpliceScan outperforms all contemporary ab initio gene structural prediction tools on the set of 5' UTR gene fragments. Conclusion Designed methods have many attractive properties, compared to existing approaches. Bayesian sensor, MHMMotif program and SpliceScan tools are freely available on our web site. Reviewers This article was reviewed by Manyuan Long, Arcady Mushegian and Mikhail Gelfand.

  2. MANAJEMEN RISIKO DAN KINERJA LABA : STUDI KASUS PADA BANK UMUM SYARIAH (BUS)

    OpenAIRE

    -, RAHMAT NURUL PRIMA. N

    2016-01-01

    2016 Manajemen Risiko dan Kinerja Laba : Studi Kasus Pada Bank Umum Syariah (BUS) Rahmat Nurul Prima Muhammad Sobarsyah Nur Alamsyah Penelitian ini bertujuan untuk menguji pengaruh implementasi manajemen risiko terhadap kinerja laba pada bank umum syariah (BUS) yang diproksikan dengan ROE. Spektrum implementasi manajemen risiko yang dijadikan objek penelitian adalah risiko kredit yang diproksikan dengan NPF, risiko likuiditas yang diproksikan dengan F...

  3. Evoking Emotions and Unpacking Layered Histories through Young Children's Illustrations of Racial Bus Segregation

    Science.gov (United States)

    Kuby, Candace R.

    2013-01-01

    Drawing on theories of multi-modality and critical visual literacy, this article focuses on images that five-and six year-olds painted in a class-made book, Voice on the Bus, about racial segregation. The article discusses how children used illustrations to convey their understandings of Rosa Parks' bus arrest in Alabama. A post-structural view…

  4. Split, Send, Reassemble: A Formal Specification of a CAN Bus Protocol Stack

    OpenAIRE

    van Glabbeek, Rob; Höfner, Peter

    2017-01-01

    We present a formal model for a fragmentation and a reassembly protocol running on top of the standardised CAN bus, which is widely used in automotive and aerospace applications. Although the CAN bus comes with an in-built mechanism for prioritisation, we argue that this is not sufficient and provide another protocol to overcome this shortcoming.

  5. Desain Dan Implementasi Sistem Komunikasi Prosesor Parallel Pada Aplikasi Robotik Berbasis Bus Serial I2C

    OpenAIRE

    Mubarak, Tito; Sumaryo, Sony; Abdurohman, Maman

    2008-01-01

    Dewasa ini desain sistem embedded tidak cukup lagi hanya dengan menerapkan satu buahmicroprocessor atau microcontroller saja. Karena sistem yang dikontrol mempunyai peralatan dan tugas yangsemakin banyak. Sehingga perlu menerapkan desentralisasi dan paralelisme pada sistem berbasismicroprocessor.Pada Penelitian ini, dirancang suatu sistem komunikasi antar microcontroller sebagai implementasi darimicroprocessor. Yaitu berupa suatu sistem bus yang berbasis pada I2C serial bus yang dikeluarkan o...

  6. Operating Time Division for a Bus Route Based on the Recovery of GPS Data

    Directory of Open Access Journals (Sweden)

    Jian Wang

    2017-01-01

    Full Text Available Bus travel time is an important source of data for time of day partition of the bus route. However, in practice, a bus driver may deliberately speed up or slow down on route so as to follow the predetermined timetable. The raw GPS data collected by the GPS device equipped on the bus, as a result, cannot reflect its real operating conditions. To address this concern, this study first develops a method to identify whether there is deliberate speed-up or slow-down movement of a bus. Building upon the relationships between the intersection delay, link travel time, and traffic flow, a recovery method is established for calculating the real bus travel time. Using the dwell time at each stop and the recovered travel time between each of them as the division indexes, a sequential clustering-based time of day partition method is proposed. The effectiveness of the developed method is demonstrated using the data of bus route 63 in Harbin, China. Results show that the partition method can help bus enterprises to design reasonable time of day intervals and significantly improve their level of service.

  7. Successfulness of bus rapid transit systems in Asia. Ex-post evaluation

    NARCIS (Netherlands)

    Kogdenko, Nadja

    2010-01-01

    SUMMARY Bus Rapid Transit (BRT) can be defined as “a bus-based mass transit system that delivers fast, comfortable, and cost-effective urban mobility”. It offers the opportunity to create a high-quality mass transit system at affordable costs, which is p

  8. Survey on the implementation and reliability of CubeSat electrical bus interfaces

    Science.gov (United States)

    Bouwmeester, Jasper; Langer, Martin; Gill, Eberhard

    2017-06-01

    This paper provides results and conclusions on a survey on the implementation and reliability aspects of CubeSat bus interfaces, with an emphasis on the data bus and power distribution. It provides recommendations for a future CubeSat bus standard. The survey is based on a literature study and a questionnaire representing 60 launched CubeSats and 44 to be launched CubeSats. It is found that the bus interfaces are not the main driver for mission failures. However, it is concluded that the Inter Integrated Circuit (I2C) data bus, as implemented in a great majority of the CubeSats, caused some catastrophic satellite failures and a vast amount of bus lockups. The power distribution may lead to catastrophic failures if the power lines are not protected against overcurrent. A connector and wiring standard widely implemented in CubeSats is based on the PC/104 standard. Most participants find the 104 pin connector of this standard too large. For a future CubeSat bus interface standard, it is recommended to implement a reliable data bus, a power distribution with overcurrent protection and a wiring harness with smaller connectors compared with PC/104.

  9. Telemetry Standards, RCC Standard 106-17. Chapter 8. Digital Data Bus Acquisition Formatting Standard

    Science.gov (United States)

    2017-07-01

    generated for the resulting formatted word or an additional bit appended to the bus/group identification label as described in Paragraph 8.2.3. 8.2.3...shall be identical to that described in Paragraph 8.2. (5) The frame length shall be fixed and shall be the same for each track used for a given bus

  10. 75 FR 71792 - National Express Corporation-Control Exemption-Vogel Bus Company, Inc.

    Science.gov (United States)

    2010-11-24

    ... passenger carrier (MC-274520). NEC indirectly owns and controls one interstate motor passenger carrier... Surface Transportation Board National Express Corporation--Control Exemption--Vogel Bus Company, Inc... approval requirements of 49 U.S.C. 14303(a)(5) and 49 CFR 1182 to acquire control of Vogel Bus Company, Inc...

  11. Bus Operation Monitoring Oriented Public Transit Travel Index System and Calculation Models

    Directory of Open Access Journals (Sweden)

    Jiancheng Weng

    2013-01-01

    Full Text Available This study proposed a two-dimensional index system which is concerned essentially with urban travel based on travel modes and user satisfaction. First, the public transit was taken as an example to describe the index system establishing process. In consideration of convenience, rapid, reliability, comfort, and safety, a bus service evaluation index system was established. The indicators include the N-minute coverage of bus stops, average travel speed, and fluctuation of travel time between stops and bus load factor which could intuitively describe the characteristics of public transport selected to calculate bus travel indexes. Then, combined with the basic indicators, the calculation models of Convenience Index (CI, Rapid Index (RI, Reliability Index (RBI, and Comfort Index (CTI were established based on the multisource data of public transit including the real-time bus GPS data and passenger IC card data. Finally, a case study of Beijing bus operation evaluation and analysis was conducted by taking real bus operation data including GPS data and passenger transaction recorder (IC card data. The results showed that the operation condition of the public transit was well reflected and scientifically classified by the bus travel index models.

  12. Drive Cycle Analysis, Measurement of Emissions and Fuel Consumption of a PHEV School Bus: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Barnitt, R.; Gonder, J.

    2011-04-01

    The National Renewable Energy Laboratory (NREL) collected and analyzed real-world school bus drive cycle data and selected similar standard drive cycles for testing on a chassis dynamometer. NREL tested a first-generation plug-in hybrid electric vehicle (PHEV) school bus equipped with a 6.4L engine and an Enova PHEV drive system comprising a 25-kW/80 kW (continuous/peak) motor and a 370-volt lithium ion battery pack. A Bluebird 7.2L conventional school bus was also tested. Both vehicles were tested over three different drive cycles to capture a range of driving activity. PHEV fuel savings in charge-depleting (CD) mode ranged from slightly more than 30% to a little over 50%. However, the larger fuel savings lasted over a shorter driving distance, as the fully charged PHEV school bus would initially operate in CD mode for some distance, then in a transitional mode, and finally in a charge-sustaining (CS) mode for continued driving. The test results indicate that a PHEV school bus can achieve significant fuel savings during CD operation relative to a conventional bus. In CS mode, the tested bus showed small fuel savings and somewhat higher nitrogen oxide (NOx) emissions than the baseline comparison bus.

  13. Development of bus-stop time models in dense urban areas : a case study in Washington DC.

    Science.gov (United States)

    2015-08-01

    Bus transit reliability depends on several factors including the route of travel, traffic conditions, time of day, and conditions at : the bus stops along the route. The number of passengers alighting or boarding, fare payment method, dwell time (DT)...

  14. A Study of BUS Architecture Design for Controller of Nuclear Power Plant Using FPGA

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dongil; Yun, Donghwa; Hwang, Sungjae; Kim, Myeongyun; Lee, Dongyun [PONUTech Co. Ltd., Seoul (Korea, Republic of)

    2014-05-15

    CPU (Central Processing Unit) operating speed and communication rate have been more technically improved than before. However, whole system is been a degradation of performance by electronic and structural limitation of parallel bus. Transmission quantity and speed have a limit and need arbiter in order to do arbitration because several boards shared parallel bus. Arbiter is a high complexity in implementing so it increases component per chip. If a parallel bus uses, it will occurs some problems what are reflection noise, power/ground noise (or ground bounce) as SSN (Simultaneous Switching Noise) and crosstalk noise like magnetic coupling. In this paper, in order to solve a problem of parallel bus in controller of NPP (Nuclear Power Plant), proposes the bus architecture design using FPGA (Field Programmable Gate Array) based on LVDS (Low Voltage Differential Signaling)

  15. Occupational safety conditions of bus drivers in Metro Manila, the Philippines.

    Science.gov (United States)

    Santos, Jewelle Ann; Lu, Jinky Leilanie

    2016-12-01

    The study looks into the occupational safety and working conditions among bus drivers in Metro Manila, the Philippines. Quantitative data were collected through survey interviews of 95 bus drivers using the stratified sampling technique. Results showed that bus drivers worked an average of 16 h/day and were engaged in risky driving behaviors such as over-speeding and road racing in order to reach their quota for the day. Fifty-nine percent experienced work-related accidents, with a mean of three accidents. The most common accident was hitting another vehicle followed by side swipe. The accidents were blamed on other drivers, followed by vehicle defect, inattentiveness and tiredness/micro-sleep or sudden involuntary sleep while driving. The most common health symptoms experienced by the bus drivers were fatigue, back pain, and cough and colds. This study underlines the need for an occupational health and safety program for bus drivers in the Philippines.

  16. Bus driving assistance system for town area by using ATmega328P microcontroller

    Science.gov (United States)

    Zakaria, Mohamad Fauzi; Soon, Tan Jiah; Rohani, Munzilah Md

    2017-09-01

    Recently, several bus accidents happened because of bus driver's behavior. In fact, there is no dedicated tool for assisting them to drive safely. This project gives solutions to this by assisting the driver, according to the speed and acceleration of the bus. These data are collected by using a motion processing unit (MPU-6050) and a global positioning system (GPS) and then indicate the driving mode status on the LEDs. All data and status are recorded in a secure digital (SD) card for the authority or the bus company to analyze the driving behavior of a bus driver. This system has been successfully developed and tested in two different areas which includes the UTHM main campus and the road from Parit Raja to Batu Pahat.

  17. Computation on Bus Delay at Stops in Beijing through Statistical Analysis

    Directory of Open Access Journals (Sweden)

    Shaokuan Chen

    2013-01-01

    Full Text Available Delays at bus stops have seriously affected the efficiency of bus operation and the improvement of level of services of public transportation and greatly influenced the preferences of passengers to choose bus services. In this paper, the analysis on arriving, dwell and leaving process of buses and the method to calculate bus delays at stops are proposed according to survey data from three bus routes in Beijing. Statistical analysis is then adopted respectively to evaluate average times that buses are docking at curbside and bay-style stops. Moreover, it is noted that different load factors of passengers in buses have significantly influenced the average boarding and alighting time per person. The effectiveness and accuracy of the proposed methods is illustrated through case studies. This study is crucial and helpful for the planners and operators to evaluate the efficiency and level of service of urban public transportation.

  18. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Second Results Report and Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-10-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This is the second results report for the AT fuel cell bus since it was placed in service, and it focuses on the newest data analysis and lessons learned since the previous report. The appendices, referenced in the main report, provide the full background for the evaluation. They will be updated as new information is collected but will contain the original background material from the first report.

  19. Cultural Factors Related to Smoking in San Francisco's Irish Bars

    Science.gov (United States)

    Satterlund, Travis D.; Antin, Tamar M. J.; Lee, Juliet P.; Moore, Roland S.

    2009-01-01

    California's Smoke-Free Workplace Act was extended to include bars in 1998. While the majority of bars in the state have become smoke free, in many bars patrons and staff continue to smoke despite the law. The authors present findings from a study which assessed cultural factors related to continued smoking in bars in the city of San Francisco. In…

  20. Exploiting the Intron-splicing Mechanism of Insect Cells to Produce Viral Vectors Harboring Toxic Genes for Suicide Gene Therapy

    Directory of Open Access Journals (Sweden)

    Haifeng Chen

    2012-01-01

    Full Text Available Two mammalian introns, the human growth hormone intron and the Simian virus 40 large T antigen intron, were inserted into the coding sequences of diphtheria toxin fragment A (DT-A and barnase (Bar, respectively, to disrupt their open-reading frames (ORFs. Expression of these two toxic proteins were totally abolished, which enabled the production of normal levels of recombinant baculoviral and adeno-associated viral (AAV vectors in insect cells. When these viral vectors were introduced into mammalian cells, the introns were spliced out and the toxic proteins were expressed, which resulted in apoptosis in mammalian cells. This is the first report to show that viral vectors harboring toxin genes can be produced at normal levels by exploiting the intron-splicing mechanism of insect cells. Furthermore, viral vectors carrying the DT-A gene under control of tumor-specific promoters were able to exert tumor-specific cell killing. This novel method to produce viral vectors harboring toxic genes under control of tumor-specific promoter offers a powerful tool for further research, as well as for the development of toxin-based suicide gene therapy drugs.

  1. Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing.

    Directory of Open Access Journals (Sweden)

    Yuanyuan Xiao

    2005-09-01

    Full Text Available Splicing is an important process for regulation of gene expression in eukaryotes, and it has important functional links to other steps of gene expression. Two examples of these linkages include Ceg1, a component of the mRNA capping enzyme, and the chromatin elongation factors Spt4-5, both of which have recently been shown to play a role in the normal splicing of several genes in the yeast Saccharomyces cerevisiae. Using a genomic approach to characterize the roles of Spt4-5 in splicing, we used splicing-sensitive DNA microarrays to identify specific sets of genes that are mis-spliced in ceg1, spt4, and spt5 mutants. In the context of a complex, nested, experimental design featuring 22 dye-swap array hybridizations, comprising both biological and technical replicates, we applied five appropriate statistical models for assessing differential expression between wild-type and the mutants. To refine selection of differential expression genes, we then used a robust model-synthesizing approach, Differential Expression via Distance Synthesis, to integrate all five models. The resultant list of differentially expressed genes was then further analyzed with regard to select attributes: we found that highly transcribed genes with long introns were most sensitive to spt mutations. QPCR confirmation of differential expression was established for the limited number of genes evaluated. In this paper, we showcase splicing array technology, as well as powerful, yet general, statistical methodology for assessing differential expression, in the context of a real, complex experimental design. Our results suggest that the Spt4-Spt5 complex may help coordinate splicing with transcription under conditions that present kinetic challenges to spliceosome assembly or function.

  2. Convergent origins and rapid evolution of spliced leader trans-splicing in metazoa: insights from the ctenophora and hydrozoa.

    Science.gov (United States)

    Derelle, Romain; Momose, Tsuyoshi; Manuel, Michael; Da Silva, Corinne; Wincker, Patrick; Houliston, Evelyn

    2010-04-01

    Replacement of mRNA 5' UTR sequences by short sequences trans-spliced from specialized, noncoding, spliced leader (SL) RNAs is an enigmatic phenomenon, occurring in a set of distantly related animal groups including urochordates, nematodes, flatworms, and hydra, as well as in Euglenozoa and dinoflagellates. Whether SL trans-splicing has a common evolutionary origin and biological function among different organisms remains unclear. We have undertaken a systematic identification of SL exons in cDNA sequence data sets from non-bilaterian metazoan species and their closest unicellular relatives. SL exons were identified in ctenophores and in hydrozoan cnidarians, but not in other cnidarians, placozoans, or sponges, or in animal unicellular relatives. Mapping of SL absence/presence obtained from this and previous studies onto current phylogenetic trees favors an evolutionary scenario involving multiple origins for SLs during eumetazoan evolution rather than loss from a common ancestor. In both ctenophore and hydrozoan species, multiple SL sequences were identified, showing high sequence diversity. Detailed analysis of a large data set generated for the hydrozoan Clytia hemisphaerica revealed trans-splicing of given mRNAs by multiple alternative SLs. No evidence was found for a common identity of trans-spliced mRNAs between different hydrozoans. One feature found specifically to characterize SL-spliced mRNAs in hydrozoans, however, was a marked adenosine enrichment immediately 3' of the SL acceptor splice site. Our findings of high sequence divergence and apparently indiscriminate use of SLs in hydrozoans, along with recent findings in other taxa, indicate that SL genes have evolved rapidly in parallel in diverse animal groups, with constraint on SL exon sequence evolution being apparently rare.

  3. Spontaneous formation of double bars in dark matter dominated galaxies

    OpenAIRE

    Saha, Kanak; Maciejewski, Witold

    2013-01-01

    Although nearly one-third of barred galaxies host an inner, secondary bar, the formation and evolution of double barred galaxies remain unclear. We show here an example model of a galaxy, dominated by a live dark matter halo, in which double bars form naturally, without requiring gas, and we follow its evolution for a Hubble time. The inner bar in our model galaxy rotates almost as slowly as the outer bar, and it can reach up to half of its length. The route to the formation of a double bar m...

  4. Two new splice variants in porcine PPARGC1A

    Directory of Open Access Journals (Sweden)

    Peelman Luc J

    2008-12-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptor γ coactivator 1α (PPARGC1A is a coactivator with a vital and central role in fat and energy metabolism. It is considered to be a candidate gene for meat quality in pigs and is involved in the development of obesity and diabetes in humans. How its many functions are regulated, is however still largely unclear. Therefore a transcription profile of PPARGC1A in 32 tissues and 4 embryonic developmental stages in the pig was constructed by screening its cDNA for possible splice variants with exon-spanning primers. Findings This led to the discovery of 2 new splice variants in the pig, which were subsequently also detected in human tissues. In these variants, exon 8 was either completely or partly (the last 66 bp were conserved spliced out, potentially coding for a much shorter protein of respectively 337 and 359 amino acids (aa, of which the first 291 aa would be the same compared to the complete protein (796 aa. Conclusion Considering the functional domains of the PPARGC1A protein, it is very likely these splice variants considerably affect the function of the protein and alternative splicing could be one of the mechanisms by which the diverse functions of PPARGC1A are regulated.

  5. Alternative Splicing Regulates Targeting of Malate Dehydrogenase in Yarrowia lipolytica

    Science.gov (United States)

    Kabran, Philomène; Rossignol, Tristan; Gaillardin, Claude; Nicaud, Jean-Marc; Neuvéglise, Cécile

    2012-01-01

    Alternative pre-mRNA splicing is a major mechanism contributing to the proteome complexity of most eukaryotes, especially mammals. In less complex organisms, such as yeasts, the numbers of genes that contain introns are low and cases of alternative splicing (AS) with functional implications are rare. We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica. The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism. This ubiquitous enzyme is involved in the tricarboxylic acid cycle in mitochondria and in the glyoxylate cycle, which takes place in peroxisomes and the cytosol. In Saccharomyces cerevisiae, three genes encode three compartment-specific enzymes. In contrast, only two genes exist in Y. lipolytica. One gene (YlMDH1, YALI0D16753g) encodes a predicted mitochondrial protein, whereas the second gene (YlMDH2, YALI0E14190g) generates the cytosolic and peroxisomal forms through the alternative use of two 3′-splice sites in the second intron. Both splicing variants were detected in cDNA libraries obtained from cells grown under different conditions. Mutants expressing the individual YlMdh2p isoforms tagged with fluorescent proteins confirmed that they localized to either the cytosolic or the peroxisomal compartment. PMID:22368181

  6. Comparison of splice sites in mammals and chicken.

    Science.gov (United States)

    Abril, Josep F; Castelo, Robert; Guigó, Roderic

    2005-01-01

    We have carried out an initial analysis of the dynamics of the recent evolution of the splice-sites sequences on a large collection of human, rodent (mouse and rat), and chicken introns. Our results indicate that the sequences of splice sites are largely homogeneous within tetrapoda. We have also found that orthologous splice signals between human and rodents and within rodents are more conserved than unrelated splice sites, but the additional conservation can be explained mostly by background intron conservation. In contrast, additional conservation over background is detectable in orthologous mammalian and chicken splice sites. Our results also indicate that the U2 and U12 intron classes seem to have evolved independently since the split of mammals and birds; we have not been able to find a convincing case of interconversion between these two classes in our collections of orthologous introns. Similarly, we have not found a single case of switching between AT-AC and GT-AG subtypes within U12 introns, suggesting that this event has been a rare occurrence in recent evolutionary times. Switching between GT-AG and the noncanonical GC-AG U2 subtypes, on the contrary, does not appear to be unusual; in particular, T to C mutations appear to be relatively well tolerated in GT-AG introns with very strong donor sites.

  7. Control of human papillomavirus gene expression by alternative splicing.

    Science.gov (United States)

    Graham, Sheila V; Faizo, Arwa Ali A

    2017-03-02

    Human papillomaviruses possess circular double stranded DNA genomes of around 8kb in size from which multiple mRNAs are synthesized during an infectious life cycle. Although at least three viral promoters are used to initiate transcription, viral mRNAs are largely the product of processing of pre-mRNAs by alternative splicing and polyadenylation. The HPV life cycle and viral gene expression are tightly linked to differentiation of the epithelium the virus infects: there is an orchestrated production of viral mRNAs and proteins. In this review we describe viral mRNA expression and the roles of the SR and hnRNP proteins that respectively positively and negatively regulate splicing. We discuss HPV regulation of splicing factors and detail the evidence that the papillomavirus E2 protein has splicing-related activities. We highlight the possibility that HPV-mediated control of splicing in differentiating epithelial cells may be necessary to accomplish the viral replication cycle. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  8. A Challenging Pie to Splice: Drugging the Spliceosome.

    Science.gov (United States)

    León, Brian; Kashyap, Manoj K; Chan, Warren C; Krug, Kelsey A; Castro, Januario E; La Clair, James J; Burkart, Michael D

    2017-09-25

    Since its discovery in 1977, the study of alternative RNA splicing has revealed a plethora of mechanisms that had never before been documented in nature. Understanding these transitions and their outcome at the level of the cell and organism has become one of the great frontiers of modern chemical biology. Until 2007, this field remained in the hands of RNA biologists. However, the recent identification of natural product and synthetic modulators of RNA splicing has opened new access to this field, allowing for the first time a chemical-based interrogation of RNA splicing processes. Simultaneously, we have begun to understand the vital importance of splicing in disease, which offers a new platform for molecular discovery and therapy. As with many natural systems, gaining clear mechanistic detail at the molecular level is key towards understanding the operation of any biological machine. This minireview presents recent lessons learned in this emerging field of RNA splicing chemistry and chemical biology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Cadmium restores in vitro splicing activity inhibited by zinc-depletion

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Myeong Jin; Ayaki, Hitoshi; Kitamura, Keiko; Nishio, Hisahide [Kobe University Graduate School of Medicine, Department of Public Health, Kobe (Japan); Goji, Junko [Hyogo College of Medicine, Department of Public Health, Hyogo (Japan)

    2006-10-15

    Zinc (Zn)-depletion inhibits the second step of RNA splicing, namely exon-ligation. To investigate the effects of cadmium (Cd) and other metal ions on RNA splicing inhibited by Zn-depletion, we measured in vitro splicing activities in the presence of these metals. Zn-depletion in the splicing reaction mixture was achieved by addition of a Zn-chelator, 1,10-phenanthroline. Cd(II) at 1, 5 and 10 {mu}M restored the splicing activity to 2, 24 and 72% of that in the control reaction mixture, while higher concentrations of Cd(II) decreased the splicing activity, and more than 50 {mu}M Cd(II) showed a complete absence of spliced products. Hg(II) also restored the splicing activity, albeit to a lesser extent, since 5 and 10 {mu}M Hg(II) restored the splicing activity to 3 and 4% of the control value. The other metal ions examined in this study, Co(II), Cu(II), Mg(II) and Mn(II), did not show any restoration of the splicing activity. We concluded that Cd(II) could restore the in vitro splicing activity inhibited by Zn-depletion, although higher concentrations of Cd(II) prevented progress of the RNA splicing reaction. These results suggest that Cd(II) has a bifunctional property regarding RNA splicing, and is stimulatory at low concentrations and inhibitory at high concentrations. (orig.)

  10. Developing fruit-based nutritious snack bars.

    Science.gov (United States)

    da Silva, Edson P; Siqueira, Heloisa H; do Lago, Rafael Carvalho; Rosell, Cristina M; Vilas Boas, Eduardo V de Barros

    2014-01-15

    Marolo (Annona crassiflora Mart) is a typical savannah fruit that is very nutritious and highly appreciated. However, its consumption has been limited to fresh fruit. The objective of this study was to evaluate the potential use of marolo flour in the production of healthy snack bars to valorise this fruit and provide an alternative ready-to-eat nutritious product. Snack bars containing increasing amounts of marolo flour (5 g 100 g(-1), 10 g 100 g(-1), 15 g 100 g(-1), 20 g 100 g(-1), expressed in w/w) were produced and the physico-chemical and sensory characteristics were determined. Levels up to 20% marolo flour can be incorporated in snack bars with some minor changes in pH and moisture content but with an increase of 2.4-fold in dietary fibre content and also 1.3-fold of vitamin C, minerals and antioxidant activity. In addition, up to 10% marolo flour improves significantly the sensory properties of the snack bars, namely appearance, taste, texture and overall acceptance. Marolo flour can be considered an alternative flour for obtaining healthy snack bars, with increased nutritional and sensory quality. © 2013 Society of Chemical Industry.

  11. Subsurface flow in lowland river gravel bars

    Science.gov (United States)

    Bray, E. N.; Dunne, T.

    2017-09-01

    Geomorphic and hydraulic processes, which form gravel bars in large lowland rivers, have distinctive characteristics that control the magnitude and spatial patterns of infiltration and exfiltration between rivers and their immediate subsurface environments. We present a bedform-infiltration relation together with a set of field measurements along two reaches of the San Joaquin River, CA to illustrate the conditions required for infiltration and exfiltration of flow between a stream and its undulating bed, and a numerical model to investigate the factors that affect paths and residence times of flow through barforms at different discharges. It is shown that asymmetry of bar morphology is a first-order control on the extent and location of infiltration, which would otherwise produce equal areas of infiltration and exfiltration under the assumption of sinusoidal bedforms. Hydraulic conductivity varies by orders of magnitude due to fine sediment accumulation and downstream coarsening related to the process of bar evolution. This systematic variability not only controls the magnitude of infiltration, but also the residence time of flow through the bed. The lowest hydraulic conductivity along the reach occurred where the difference between the topographic gradient and the water-surface gradient is at a maximum and thus where infiltration would be greatest into a homogeneous bar, indicating the importance of managing sand supply to maintain the ventilation and flow through salmon spawning riffles. Numerical simulations corroborate our interpretation that infiltration patterns and rates are controlled by distinctive features of bar morphology.

  12. Numerical simulations of wave propagation in long bars with application to Kolsky bar testing

    Energy Technology Data Exchange (ETDEWEB)

    Corona, Edmundo [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2014-11-01

    Material testing using the Kolsky bar, or split Hopkinson bar, technique has proven instrumental to conduct measurements of material behavior at strain rates in the order of 103 s-1. Test design and data reduction, however, remain empirical endeavors based on the experimentalist's experience. Issues such as wave propagation across discontinuities, the effect of the deformation of the bar surfaces in contact with the specimen, the effect of geometric features in tensile specimens (dog-bone shape), wave dispersion in the bars and other particulars are generally treated using simplified models. The work presented here was conducted in Q3 and Q4 of FY14. The objective was to demonstrate the feasibility of numerical simulations of Kolsky bar tests, which was done successfully.

  13. Results from the KARMEN nu-bar submu -> nu-bar sub e neutrino oscillation search

    CERN Document Server

    Eitel, K

    1999-01-01

    The neutrino experiment KARMEN is situated at the beam stop neutrino source ISIS. It provides nu submu's, nu sub e 's and nu-bar's in equal intensities from the pi sup + -mu sup + -decay at rest (DAR). The oscillation channel nu-bar submu -> nu-bar sub e is investigated in the appearance mode with a 56 t liquid scintillation calorimeter at a mean distance of 17.7 m from the nu-source looking for p (nu-bar sub e , e sup +) n reactions. In the data collected through 1997 and 1998 no potential oscillation event was observed. Using a unified approach to small signals as recommended by the PDG, this leads to an upper limit for the mixing angle of sin sup 2 (2 THETA) nu-bar sub e evidence.

  14. Novel low cost standardized Nano-Satellite structure bus for LEO missions

    Science.gov (United States)

    Anubhav, T.; Sarwesh, P.; Narayan, V.; Varma, P. A.; Prasad, R. A.; Loganathan, M.; Rao, D. N.; Sriram, S.; Venkatesh, M.

    This paper focuses on SRMSAT STRUCTURE BUS which is a standardized Nano-Satellite structure bus. It provides a standard platform for a wide variety of missions in LEO and can be realized in a very short developmental period. The bus was designed and developed for SRMSAT, the SRM University (Sri Ramaswamy Memorial University) student Nano-Satellite, by the undergraduate students and faculty of SRM University in collaboration with Indian Space Research Organization (ISRO). The bus can support payloads up to a mass of 20kg. SRMSAT STRUCTURE BUS has a mass of around 6 kg and dimensions 280mm × 280mm × 280mm with an available volume of 11000 cc. Vibration Testing of the bus has been performed upto 6.7 gRMS. This makes the satellite capable of being launched by any launch vehicle in the world. An innovative PCB mounting design has been introduced in this structure bus which facilitates mounting of a maximum 7 PCB trays independently, each tray capable of holding a 250 mm2 PCB. Structural analysis of SRMSAT STRUCTURE BUS was done using NX Nastran. The boundary conditions for each analysis were defined based on the loading conditions as specified by the launcher, PSLV (Polar Satellite Launch Vehicle). Optimization of each individual component was performed by maintaining a minimum threshold between the local frequencies of the component and global frequencies of the entire satellite. Static, Modal, Harmonic and Random Vibration analysis of the structure bus was performed. This paper also describes the methodology followed in the static and dynamic analysis of the structure bus to finalize the design. The results have been tested and validated at ISRO Satellite Centre, Bangalore with around 90% accuracy and the structure has been certified as a standard structure bus for Nano-satellite missions. In terms of expandability, this structure bus is capable of accommodating deployable solar panels. Also, the payload mounting is not only restricted to th- bottom deck but can

  15. Widespread evolutionary conservation of alternatively spliced exons in caenorhabditis

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Penny, David

    2007-01-01

    Alternative splicing (AS) contributes to increased transcriptome and proteome diversity in various eukaryotic lineages. Previous studies showed low levels of conservation of alternatively spliced (cassette) exons within mammals and within dipterans. We report a strikingly different pattern...... in Caenorhabditis nematodes-more than 92% of cassette exons from Caenorhabditis elegans are conserved in Caenorhabditis briggsae and/or Caenorhabditis remanei. High levels of conservation extend to minor-form exons (present in a minority of transcripts) and are particularly pronounced for exons showing complex...... patterns of splicing. The functionality of the vast majority of cassette exons is underscored by various other features. We suggest that differences in conservation between lineages reflect differences in levels of functionality and further suggest that these differences are due to differences in intron...

  16. Minor class splicing shapes the zebrafish transcriptome during development

    DEFF Research Database (Denmark)

    Markmiller, Sebastian; Cloonan, Nicole; Lardelli, Rea M

    2014-01-01

    as the U11/U12 di-snRNP 65-kDa protein, a unique component of the U12-type spliceosome. The biochemical impact of the mutation in clbn is the formation of aberrant U11- and U12-containing small nuclear ribonucleoproteins that impair the efficiency of U12-type splicing. Using RNA sequencing and microarrays....... Analysis of its transcriptome reveals efficient mRNA processing as a critical process for the growth and proliferation of cells during vertebrate development.......Minor class or U12-type splicing is a highly conserved process required to remove a minute fraction of introns from human pre-mRNAs. Defects in this splicing pathway have recently been linked to human disease, including a severe developmental disorder encompassing brain and skeletal abnormalities...

  17. Identifying splicing regulatory elements with de Bruijn graphs.

    Science.gov (United States)

    Badr, Eman; Heath, Lenwood S

    2014-12-01

    Splicing regulatory elements (SREs) are short, degenerate sequences on pre-mRNA molecules that enhance or inhibit the splicing process via the binding of splicing factors, proteins that regulate the functioning of the spliceosome. Existing methods for identifying SREs in a genome are either experimental or computational. Here, we propose a formalism based on de Bruijn graphs that combines genomic structure, word count enrichment analysis, and experimental evidence to identify SREs found in exons. In our approach, SREs are not restricted to a fixed length (i.e., k-mers, for a fixed k). As a result, we identify 2001 putative exonic enhancers and 3080 putative exonic silencers for human genes, with lengths varying from 6 to 15 nucleotides. Many of the predicted SREs overlap with experimentally verified binding sites. Our model provides a novel method to predict variable length putative regulatory elements computationally for further experimental investigation.

  18. Stabilized cyclopropane analogs of the splicing inhibitor FD-895.

    Science.gov (United States)

    Villa, Reymundo; Kashyap, Manoj Kumar; Kumar, Deepak; Kipps, Thomas J; Castro, Januario E; La Clair, James J; Burkart, Michael D

    2013-09-12

    Targeting the spliceosome with small molecule inhibitors provides a new avenue to target cancer by intercepting alternate splicing pathways. Although our understanding of alternate mRNA splicing remains poorly understood, it provides an escape pathway for many cancers resistant to current therapeutics. These findings have encouraged recent academic and industrial efforts to develop natural product spliceosome inhibitors, including FD-895 (1a), pladienolide B (1b), and pladienolide D (1c), into next-generation anticancer drugs. The present study describes the application of semisynthesis and total synthesis to reveal key structure-activity relationships for the spliceosome inhibition by 1a. This information is applied to deliver new analogs with improved stability and potent activity at inhibiting splicing in patient derived cell lines.

  19. Alternative splice variants of the human PD-1 gene

    DEFF Research Database (Denmark)

    Nielsen, Christian; Ohm-Laursen, Line; Barington, Torben

    2005-01-01

    PD-1 is an immunoregulatory receptor expressed on the surface of activated T cells, B cells, and monocytes. We describe four alternatively spliced PD-1 mRNA transcripts (PD-1Deltaex2, PD-1Deltaex3, PD-1Deltaex2,3, and PD-1Deltaex2,3,4) in addition to the full length isoform. PD-1Deltaex2 and PD-1......Deltaex3 are generated by alternative splicing where exon 2 (extracellular IgV-like domain) and exon 3 (transmembrane domain) respectively are spliced out. PD-1Deltaex3 is therefore likely to encode a soluble form of PD-1. PD-1Deltaex2,3 lacks exon 2 and 3. These three variants have unaffected open...

  20. Global Genetic Robustness of the Alternative Splicing Machinery in Caenorhabditis elegans

    NARCIS (Netherlands)

    Li, Yang; Breitling, Rainer; Snoek, L. Basten; van der Velde, K. Joeri; Swertz, Morris A.; Riksen, Joost; Jansen, Ritsert C.; Kammenga, Jan E.; Borevitz, J.

    Alternative splicing is considered a major mechanism for creating multicellular diversity from a limited repertoire of genes. Here, we performed the first study of genetic variation controlling alternative splicing patterns by comprehensively identifying quantitative trait loci affecting the

  1. Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila

    National Research Council Canada - National Science Library

    Khodor, Yevgenia L; Rodriguez, Joseph; Abruzzi, Katharine C; Tang, Chih-Hang Anthony; Marr, 2nd, Michael T; Rosbash, Michael

    2011-01-01

    ..., with ∼3% being almost completely retained in nascent pre-mRNA. Although individual introns showed slight but statistically significant differences in splicing efficiency, similar global levels of splicing were seen from both sources...

  2. Cloning, expression and alternative splicing of the novel isoform of hTCP11 gene

    DEFF Research Database (Denmark)

    Ma, Yong-xin; Zhang, Si-zhong; Wu, Qia-qing

    2003-01-01

    To identify a novel isoform of hTCP11 gene and investigate its expression and alternative splicing.......To identify a novel isoform of hTCP11 gene and investigate its expression and alternative splicing....

  3. FAKTOR PENYEBAB KERUSAKAN SISTEM MESIN PADA BUS APTB PPD

    Directory of Open Access Journals (Sweden)

    Laela Chairani

    2015-06-01

    Full Text Available Comfort and safety of public transportation, particularly APTB PPD buses, becomes high expectations for its customers. Those expectations must be supported by the application of a good engine maintenance system. By using the method of Reliability Centered Maintenance may be determined appropriate actions to mitigate damage. From the analysis known the failures of the APTB PPD bus are malfunctions of the fuel engine, power transfer systems, difficulty to move the gear lever, and lubricating system for the rough engine noise. The cause of engine malfunction is dirty diesel filter, vehicle acceleration malfunction is due to the clutch plate wear out. Difficulty to move the gear lever indicates that the clutch slips. Rough engine noise caused by the flow of oil to the engine stalled as the result of dirty oil filter. The results of the logic tree analysis show that for each failure requires scheduling of time directed task.

  4. Foothill Transit Battery Electric Bus Demonstration Results: Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jeffers, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2017-06-28

    This report summarizes results of a battery electric bus (BEB) evaluation at Foothill Transit, located in the San Gabriel and Pomona Valley region of Los Angeles County, California. Foothill Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's (DOE's) National Renewable Energy Laboratory to evaluate its fleet of Proterra BEBs in revenue service. The focus of this evaluation is to compare performance of the BEBs to that of conventional technology and to track progress over time toward meeting performance targets. This project has also provided an opportunity for DOE to conduct a detailed evaluation of the BEBs and charging infrastructure. This is the second report summarizing the results of the BEB demonstration at Foothill Transit and it provides data on the buses from August 2015 through December 2016. Data are provided on a selection of compressed natural gas buses as a baseline comparison.

  5. Application of gravity model on the Korean urban bus network

    Science.gov (United States)

    Hong, Inho; Jung, Woo-Sung

    2016-11-01

    Mobility models have been studied to describe the underlying mechanism of human mobility. The mobility patterns in various transportation systems were understood with the gravity model by estimating the traffic as a simple function of population and distance. Compared to most studies on large-scale systems, we focused on the validity and characteristics of gravity model for intraurban mobility. Several variations of gravity model are applied on the urban bus systems of five medium-sized cities in Korea. The gravity model successfully estimates the intraurban traffic without universal exponents for cities. From the change of exponents by predictor types, we figure out the effect by a non-trivial relation between traffic and population in the urban areas.

  6. Using a Control System Ethernet Network as a Field Bus

    CERN Document Server

    De Van, William R; Lawson, Gregory S; Wagner, William H; Wantland, David M; Williams, Ernest

    2005-01-01

    A major component of a typical accelerator distributed control system (DCS) is a dedicated, large-scale local area communications network (LAN). The SNS EPICS-based control system uses a LAN based on the popular IEEE-802.3 set of standards (Ethernet). Since the control system network infrastructure is available throughout the facility, and since Ethernet-based controllers are readily available, it is tempting to use the control system LAN for "fieldbus" communications to low-level control devices (e.g. vacuum controllers; remote I/O). These devices may or may not be compatible with the high-level DCS protocols. This paper presents some of the benefits and risks of combining high-level DCS communications with low-level "field bus" communications on the same network, and describes measures taken at SNS to promote compatibility between devices connected to the control system network.

  7. [Bus drivers' biomechanical risk assessment in two different contexts].

    Science.gov (United States)

    Baracco, A; Coggiola, M; Perrelli, F; Banchio, M; Martignone, S; Gullino, A; Romano, C

    2012-01-01

    The application of standardize methods for the biomechanical risk assessment in non-industrial cycled activity is not always possible. A typical case is the public transport sector, where workers complain of suffering for shoulder more than elbow and wrist pains. The Authors present the results of two studies involving two public transport companies and the risk of biomechanical overload of upper limbs for bus and tram drivers. The analysis has been made using three different approaches: focus groups; static analysis by using anthropometric manikins; work sampling technique by monitoring worker's activity and posture at each minute, for two hours and for each binomial vehicle-route, considering P5F e P95M drivers and assessing the perceived efforts thorough the Borg's CR10 Scale. The conclusive results show that the ergonomic analysis managed by multiple non-standardized techniques may reach consistent and repeatable results according to the epidemiological evidences.

  8. Genome-wide survey of allele-specific splicing in humans

    Directory of Open Access Journals (Sweden)

    Scheffler Konrad

    2008-06-01

    Full Text Available Abstract Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation on the efficiency of mRNA splicing is often difficult to predict, many mutations that cause disease through an effect on splicing are likely to remain undiscovered. Results We have combined a genome-wide scan for sequence polymorphisms likely to affect mRNA splicing with analysis of publicly available Expressed Sequence Tag (EST and exon array data. The genome-wide scan uses published tools and identified 30,977 SNPs located within donor and acceptor splice sites, branch points and exonic splicing enhancer elements. For 1,185 candidate splicing polymorphisms the difference in splicing between alternative alleles was corroborated by publicly available exon array data from 166 lymphoblastoid cell lines. We developed a novel probabilistic method to infer allele-specific splicing from EST data. The method uses SNPs and alternative mRNA isoforms mapped to EST sequences and models both regulated alternative splicing as well as allele-specific splicing. We have also estimated heritability of splicing and report that a greater proportion of genes show evidence of splicing heritability than show heritability of overall gene expression level. Our results provide an extensive resource that can be used to assess the possible effect on splicing of human polymorphisms in putative splice-regulatory sites. Conclusion We report a set of genes showing evidence of allele-specific splicing from an integrated analysis of genomic polymorphisms, EST data and exon array

  9. Autonomous Navigation and Obstacle Avoidance of a Micro-Bus

    Directory of Open Access Journals (Sweden)

    Carlos Fernández

    2013-04-01

    Full Text Available At present, the topic of automated vehicles is one of the most promising research areas in the field of Intelligent Transportation Systems (ITS. The use of automated vehicles for public transportation also contributes to reductions in congestion levels and to improvements in traffic flow. Moreover, electrical public autonomous vehicles are environmentally friendly, provide better air quality and contribute to energy conservation. The driverless public transportation systems, which are at present operating in some airports and train stations, are restricted to dedicated roads and exhibit serious trouble dynamically avoiding obstacles in the trajectory. In this paper, an electric autonomous mini-bus is presented. All datasets used in this article were collected during the experiments carried out in the demonstration event of the 2012 IEEE Intelligent Vehicles Symposium that took place in Alcalá de Henares (Spain. The demonstration consisted of a route 725 metres long containing a list of latitude-longitude points (waypoints. The mini-bus was capable of driving autonomously from one waypoint to another using a GPS sensor. Furthermore, the vehicle is provided with a multi-beam Laser Imaging Detection and Ranging (LIDAR sensor for surrounding reconstruction and obstacle detection. When an obstacle is detected in the planned path, the planned route is modified in order to avoid the obstacle and continue its way to the end of the mission. On the demonstration day, a total of 196 attendees had the opportunity to get a ride on the vehicles. A total of 28 laps were successfully completed in full autonomous mode in a private circuit located in the National Institute for Aerospace Research (INTA, Spain. In other words, the system completed 20.3 km of driverless navigation and obstacle avoidance.

  10. Indonesian commercial bus drum brake system temperature model

    Energy Technology Data Exchange (ETDEWEB)

    Wibowo, D. B., E-mail: rmt.bowo@gmail.com; Haryanto, I., E-mail: ismoyo2001@yahoo.de; Laksono, N. P., E-mail: priyolaksono89@gmail.com [Mechanical Engineering Dept., Faculty of Engineering, Diponegoro University (Indonesia)

    2016-03-29

    Brake system is the most significant aspect of an automobile safety. It must be able to slow the vehicle, quickly intervening and reliable under varying conditions. Commercial bus in Indonesia, which often stops suddenly and has a high initial velocity, will raise the temperature of braking significantly. From the thermal analysis it is observed that for the bus with the vehicle laden mass of 15 tons and initial velocity of 80 km/h the temperature is increasing with time and reaches the highest temperature of 270.1 °C when stops on a flat road and reaches 311.2 °C on a declination road angle, ø, 20°. These temperatures exceeded evaporation temperature of brake oil DOT 3 and DOT 4. Besides that, the magnitude of the braking temperature also potentially lowers the friction coefficient of more than 30%. The brakes are pressed repeatedly and high-g decelerations also causes brake lining wear out quickly and must be replaced every 1 month as well as the emergence of a large thermal stress which can lead to thermal cracking or thermal fatigue crack. Brake fade phenomenon that could be the cause of many buses accident in Indonesia because of the failure of the braking function. The chances of accidents will be even greater when the brake is worn and not immediately replaced which could cause hot spots as rivets attached to the brake drum and brake oil is not changed for more than 2 years that could potentially lower the evaporation temperature because of the effect hygroscopic.

  11. Real-time bus location monitoring using Arduino

    Science.gov (United States)

    Ibrahim, Mohammad Y. M.; Audah, Lukman

    2017-09-01

    The Internet of Things (IoT) is the network of objects, such as a vehicles, mobile devices, and buildings that have electronic components, software, and network connectivity that enable them to collect data, run commands, and be controlled through the Internet. Controlling physical items from the Internet will increase efficiency and save time. The growing number of devices used by people increases the practicality of having IoT devices on the market. The IoT is also an opportunity to develop products that can save money and time and increase work efficiency. Initially, they need more efficiency for real-time bus location systems, especially in university campuses. This system can easily find the accurate locations of and distances between each bus stop and the estimated time to reach a new location. This system has been separated into two parts, which are the hardware and the software. The hardware parts are the Arduino Uno and the Global Positioning System (GPS), while Google Earth and GpsGate are the software parts. The GPS continuously takes input data from the satellite and stores the latitude and longitude values in the Arduino Uno. If we want to track the vehicle, we need to send the longitude and latitude as a message to the Google Earth software to convert these into maps for navigation. Once the Arduino Uno is activated, it takes the last received latitude and longitude positions' values from GpsGate and sends a message to Google Earth. Once the message has been sent to Google Earth, the current location will be shown, and navigation will be activated automatically. Then it will be broadcast using ManyCam, Google+ Hangouts, and YouTube, as well as Facebook, and appear to users. The additional features use Google Forms for determining problems faced by students, who can also take immediate action against the responsible department. Then after several successful simulations, the results will be shown in real time on a map.

  12. The exon junction complex differentially marks spliced junctions.

    Science.gov (United States)

    Saulière, Jérôme; Haque, Nazmul; Harms, Scot; Barbosa, Isabelle; Blanchette, Marco; Le Hir, Hervé

    2010-10-01

    The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.

  13. Chapter 1. Regulation of HIV-1 alternative RNA splicing and its role in virus replication.

    Science.gov (United States)

    Stoltzfus, C Martin

    2009-01-01

    Over 40 different human immunodeficiency virus type 1 (HIV-1) mRNA species, both completely and incompletely spliced, are produced by alternative splicing of the primary viral RNA transcript. In addition, about half of the viral RNA remains unspliced and is transported to the cytoplasm where it is used both as mRNA and as genomic RNA. In general, the identities of the completely and incompletely spliced HIV-1 mRNA species are determined by the proximity of the open reading frames to the 5'-end of the mRNAs. The relative abundance of the mRNAs encoding the HIV-1 gene products is determined by the frequency of splicing at the different alternative 3'-splice sites. This chapter will highlight studies showing how HIV-1 uses exon definition to control the level of splicing at each of its 3'-splice sites through a combination of positively acting exonic splicing enhancer (ESE) elements, negatively acting exonic and intronic splicing silencer elements (ESS and ISS elements, respectively), and the 5'-splice sites of the regulated exons. Each of these splicing elements represent binding sites for cellular factors whose levels in the infected cell can determine the dominance of the positive or negative elements on HIV-1 alternative splicing. Both mutations of HIV-1 splicing elements and overexpression or inhibition of cellular splicing factors that bind to these elements have been used to show that disruption of regulated splicing inhibits HIV-1 replication. These studies have provided strong rationale for the investigation and development of antiviral drugs that specifically inhibit HIV-1 RNA splicing.

  14. Can the HIV-1 splicing machinery be targeted for drug discovery?

    Directory of Open Access Journals (Sweden)

    Dlamini Z

    2017-03-01

    Full Text Available Zodwa Dlamini, Rodney Hull Research, Innovation & Engagements Portfolio, Mangosuthu University of Technology, Durban, South Africa Abstract: HIV-1 is able to express multiple protein types and isoforms from a single 9 kb mRNA transcript. These proteins are also expressed at particular stages of viral development, and this is achieved through the control of alternative splicing and the export of these transcripts from the nucleus. The nuclear export is controlled by the HIV protein Rev being required to transport incompletely spliced and partially spliced mRNA from the nucleus where they are normally retained. This implies a close relationship between the control of alternate splicing and the nuclear export of mRNA in the control of HIV-1 viral proliferation. This review discusses both the processes. The specificity and regulation of splicing in HIV-1 is controlled by the use of specific splice sites as well as exonic splicing enhancer and exonic splicing silencer sequences. The use of these silencer and enhancer sequences is dependent on the serine arginine family of proteins as well as the heterogeneous nuclear ribonucleoprotein family of proteins that bind to these sequences and increase or decrease splicing. Since alternative splicing is such a critical factor in viral development, it presents itself as a promising drug target. This review aims to discuss the inhibition of splicing, which would stall viral development, as an anti-HIV therapeutic strategy. In this review, the most recent knowledge of splicing in human immunodeficiency viral development and the latest therapeutic strategies targeting human immunodeficiency viral splicing are discussed. Keywords: alternative splicing, exonic splicing enhancer, exonic specific silencer, splicing based therapies, SR proteins, hnRNP, Rev, Tat, Vpr

  15. An erythroid differentiation-specific splicing switch in protein 4.1R mediated by the interaction of SF2/ASF with an exonic splicing enhancer.

    Science.gov (United States)

    Yang, Guang; Huang, Shu-Ching; Wu, Jane Y; Benz, Edward J

    2005-03-01

    Protein 4.1R is a vital component of the red blood cell membrane cytoskeleton. Promotion of cytoskeletal junctional complex stability requires an erythroid differentiation stage-specific splicing switch promoting inclusion of exon 16 within the spectrin/actin binding domain. We showed earlier that an intricate combination of positive and negative RNA elements controls exon 16 splicing. In this report, we further identified 3 putative exonic splicing enhancers within exon 16 and investigated the function of the sequence CAGACAT in the regulation of exon 16 splicing. Mutation of these sequences leads to increased exclusion of exon 16 in both in vivo and in vitro splicing assays, indicating that CAGACAT is a functional exonic splicing enhancer. UV cross-linking further detects an approximately 33-kDa protein that specifically binds to the CAGACAT-containing transcript. An anti-SF2/ASF antibody specifically immunoprecipitates the approximately 33-kDa protein. Furthermore, SF2/ASF stimulates exon 16 inclusion in both in vitro complementation assays and minigene-transfected mouse erythroleukemia cells (MELCs). Finally, SF2/ASF expression is up-regulated and correlates with exon 16 inclusion in differentiated MELCs. These results suggest that increased splicing factor 2/alternative splicing factor (SF2/ASF) expression in differentiated mouse erythroleukemia mediates a differentiation stage-specific exon 16 splicing switch through its interaction with the exonic splicing enhancer.

  16. Assembly of splicing complexes on exon 11 of the human insulin receptor gene does not correlate with splicing efficiency in-vitro

    Directory of Open Access Journals (Sweden)

    Caples Matt

    2004-07-01

    Full Text Available Abstract Background Incorporation of exon 11 of the insulin receptor gene is both developmentally and hormonally-regulated. Previously, we have shown the presence of enhancer and silencer elements that modulate the incorporation of the small 36-nucleotide exon. In this study, we investigated the role of inherent splice site strength in the alternative splicing decision and whether recognition of the splice sites is the major determinant of exon incorporation. Results We found that mutation of the flanking sub-optimal splice sites to consensus sequences caused the exon to be constitutively spliced in-vivo. These findings are consistent with the exon-definition model for splicing. In-vitro splicing of RNA templates containing exon 11 and portions of the upstream intron recapitulated the regulation seen in-vivo. Unexpectedly, we found that the splice sites are occupied and spliceosomal complex A was assembled on all templates in-vitro irrespective of splicing efficiency. Conclusion These findings demonstrate that the exon-definition model explains alternative splicing of exon 11 in the IR gene in-vivo but not in-vitro. The in-vitro results suggest that the regulation occurs at a later step in spliceosome assembly on this exon.

  17. Analysis of a botryllid enriched-full-length cDNA library: insight into the evolution of spliced leader trans-splicing in tunicates.

    Science.gov (United States)

    Gasparini, Fabio; Shimeld, Sebastian M

    2011-03-01

    In some animals, mRNA may be modified after transcription by the addition of a 5' spliced leader sequence. This is known as spliced leader (SL) trans-splicing, and is of uncertain function and evolutionary origin. Here, we report the identification of SL trans-splicing in the colonial ascidian Botryllus schlosseri. Combining our own expressed sequence tag (EST) data with additional data from GenBank, we identify the dominant spliced leader sequence and show it to be similar to that of other ascidians and to that of Oikopleura dioica, a basally diverging tunicate. Gene Ontology analysis of B. schlosseri ESTs with and without a 5' spliced leader shows that genes encoding ribosomal proteins tend not to be trans-spliced, a character shared with the ascidian Ciona intestinalis. We also examine individual cases of genes that produce mRNAs that are SL trans-spliced in B. schlosseri but not in C. intestinalis. We conclude that SL trans-splicing evolved early in the tunicate lineage and shows stability over considerable evolutionary time. However, SL trans-splicing may be gained or lost in individual genes.

  18. Implementation of a Cooking Bus intervention to support cooking in schools in Wales, UK.

    Science.gov (United States)

    Segrott, Jeremy; Holliday, Jo; Murphy, Simon; Macdonald, Sarah; Roberts, Joan; Moore, Laurence; Phillips, Ceri

    2017-01-01

    The teaching of cooking is an important aspect of school-based efforts to promote healthy diets among children, and is frequently done by external agencies. Within a limited evidence base relating to cooking interventions in schools, there are important questions about how interventions are integrated within school settings. The purpose of this paper is to examine how a mobile classroom (Cooking Bus) sought to strengthen connections between schools and cooking, and drawing on the concept of the sociotechnical network, theorise the interactions between the Bus and school contexts. Methods comprised a postal questionnaire to 76 schools which had received a Bus visit, and case studies of the Bus' work in five schools, including a range of school sizes and urban/rural locations. Case studies comprised observation of Cooking Bus sessions, and interviews with school staff. The Cooking Bus forged connections with schools through aligning intervention and schools' goals, focussing on pupils' cooking skills, training teachers and contributing to schools' existing cooking-related activities. The Bus expanded its sociotechnical network through post-visit integration of cooking activities within schools, particularly teachers' use of intervention cooking kits. The paper highlights the need for research on the long-term impacts of school cooking interventions, and better understanding of the interaction between interventions and school contexts. This paper adds to the limited evidence base on school-based cooking interventions by theorising how cooking interventions relate to school settings, and how they may achieve integration.

  19. B Counting at BaBar

    Energy Technology Data Exchange (ETDEWEB)

    McGregor, Grant Duncan

    2008-12-16

    In this thesis we examine the method of counting B{bar B} events produced in the BABAR experiment. The original method was proposed in 2000, but improvements to track reconstruction and our understanding of the detector since that date make it appropriate to revisit the B Counting method. We propose a new set of cuts designed to minimize the sensitivity to time-varying backgrounds. We find the new method counts B{bar B} events with an associated systematic uncertainty of {+-} 0.6%.

  20. Newtorites in bar detectors of gravitational wave

    CERN Document Server

    Ronga, F

    2016-01-01

    The detection of particles with only gravitational interactions (Newtorites) in gravitational bar detectors was studied in 1984 by Bernard, De Rujula and Lautrup. The negative results of dark matter searches suggest to look to exotic possibilities like Newtorites. The limits obtained with the Nautilus bar detector will be presented and the possible improvements will be discussed. Since the gravitational coupling is very weak, the possible limits are very far from what is needed for dark matter, but for large masses are the best limits obtained on the Earth. An update of limits for MACRO particles will be given.

  1. Imaging of physeal bars in children

    Energy Technology Data Exchange (ETDEWEB)

    Wang, David C. [Massachusetts General Hospital and Harvard Medical School, Department of Radiology, Boston, MA (United States); Deeney, Vincent; Roach, James W. [Children' s Hospital of Pittsburgh of UPMC, Department of Orthopedics, Pittsburgh, PA (United States); Shah, Amisha J. [Children' s Hospital of Pittsburgh of UPMC, Department of Radiology, Pittsburgh, PA (United States)

    2015-08-15

    The growth plate, also known as the physis or epiphyseal plate, is essential for longitudinal growth of bones in the immature skeleton. A variety of insults to the growth plate from trauma to infection to idiopathic causes can lead to physeal bar formation, an interruption in normal growth plate cartilage, where a bony or fibrous bridge develops between the metaphysis and epiphysis. This bridge restricts subsequent bone growth, leading to limb shortening and/or angular deformities. Early recognition of the presence of a physeal bar can help direct appropriate surgical management to restore linear growth of the bone. (orig.)

  2. Developing fruit-based nutritious snack bars

    OpenAIRE

    SILVA, E. P. da; Siqueira, H. H.; Lago, R. C. do; Rosell, Cristina M.; Vilas Boas, E. V. D. B.

    2014-01-01

    BACKGROUND: Marolo (Annona crassiflora Mart) is a typical savannah fruit that is very nutritious and highly appreciated. However, its consumption has been limited to fresh fruit. The objective of this study was to evaluate the potential use of marolo flour in the production of healthy snack bars to valorise this fruit and provide an alternative ready-to-eat nutritious product. Snack bars containing increasing amounts of marolo flour (5 g 100 g-1, 10 g 100 g-1, 15 g 100 g-1, 20 g 100 g-1, expr...

  3. ISVASE: identification of sequence variant associated with splicing event using RNA-seq data.

    Science.gov (United States)

    Aljohi, Hasan Awad; Liu, Wanfei; Lin, Qiang; Yu, Jun; Hu, Songnian

    2017-06-28

    Exon recognition and splicing precisely and efficiently by spliceosome is the key to generate mature mRNAs. About one third or a half of disease-related mutations affect RNA splicing. Software PVAAS has been developed to identify variants associated with aberrant splicing by directly using RNA-seq data. However, it bases on the assumption that annotated splicing site is normal splicing, which is not true in fact. We develop the ISVASE, a tool for specifically identifying sequence variants associated with splicing events (SVASE) by using RNA-seq data. Comparing with PVAAS, our tool has several advantages, such as multi-pass stringent rule-dependent filters and statistical filters, only using split-reads, independent sequence variant identification in each part of splicing (junction), sequence variant detection for both of known and novel splicing event, additional exon-exon junction shift event detection if known splicing events provided, splicing signal evaluation, known DNA mutation and/or RNA editing data supported, higher precision and consistency, and short running time. Using a realistic RNA-seq dataset, we performed a case study to illustrate the functionality and effectiveness of our method. Moreover, the output of SVASEs can be used for downstream analysis such as splicing regulatory element study and sequence variant functional analysis. ISVASE is useful for researchers interested in sequence variants (DNA mutation and/or RNA editing) associated with splicing events. The package is freely available at https://sourceforge.net/projects/isvase/ .

  4. Interplay between DMD Point Mutations and Splicing Signals in Dystrophinopathy Phenotypes

    Science.gov (United States)

    Juan-Mateu, Jonàs; González-Quereda, Lidia; Rodríguez, Maria José; Verdura, Edgard; Lázaro, Kira; Jou, Cristina; Nascimento, Andrés; Jiménez-Mallebrera, Cecilia; Colomer, Jaume; Monges, Soledad; Lubieniecki, Fabiana; Foncuberta, Maria Eugenia; Pascual-Pascual, Samuel Ignacio; Molano, Jesús; Baiget, Montserrat; Gallano, Pia

    2013-01-01

    DMD nonsense and frameshift mutations lead to severe Duchenne muscular dystrophy while in-frame mutations lead to milder Becker muscular dystrophy. Exceptions are found in 10% of cases and the production of alternatively spliced transcripts is considered a key modifier of disease severity. Several exonic mutations have been shown to induce exon-skipping, while splice site mutations result in exon-skipping or activation of cryptic splice sites. However, factors determining the splicing pathway are still unclear. Point mutations provide valuable information regarding the regulation of pre-mRNA splicing and elements defining exon identity in the DMD gene. Here we provide a comprehensive analysis of 98 point mutations related to clinical phenotype and their effect on muscle mRNA and dystrophin expression. Aberrant splicing was found in 27 mutations due to alteration of splice sites or splicing regulatory elements. Bioinformatics analysis was performed to test the ability of the available algorithms to predict consequences on mRNA and to investigate the major factors that determine the splicing pathway in mutations affecting splicing signals. Our findings suggest that the splicing pathway is highly dependent on the interplay between splice site strength and density of regulatory elements. PMID:23536893

  5. Interplay between DMD point mutations and splicing signals in Dystrophinopathy phenotypes.

    Directory of Open Access Journals (Sweden)

    Jonàs Juan-Mateu

    Full Text Available DMD nonsense and frameshift mutations lead to severe Duchenne muscular dystrophy while in-frame mutations lead to milder Becker muscular dystrophy. Exceptions are found in 10% of cases and the production of alternatively spliced transcripts is considered a key modifier of disease severity. Several exonic mutations have been shown to induce exon-skipping, while splice site mutations result in exon-skipping or activation of cryptic splice sites. However, factors determining the splicing pathway are still unclear. Point mutations provide valuable information regarding the regulation of pre-mRNA splicing and elements defining exon identity in the DMD gene. Here we provide a comprehensive analysis of 98 point mutations related to clinical phenotype and their effect on muscle mRNA and dystrophin expression. Aberrant splicing was found in 27 mutations due to alteration of splice sites or splicing regulatory elements. Bioinformatics analysis was performed to test the ability of the available algorithms to predict consequences on mRNA and to investigate the major factors that determine the splicing pathway in mutations affecting splicing signals. Our findings suggest that the splicing pathway is highly dependent on the interplay between splice site strength and density of regulatory elements.

  6. Four Experiments on the Perception of Bar Charts.

    Science.gov (United States)

    Talbot, Justin; Setlur, Vidya; Anand, Anushka

    2014-12-01

    Bar charts are one of the most common visualization types. In a classic graphical perception paper, Cleveland & McGill studied how different bar chart designs impact the accuracy with which viewers can complete simple perceptual tasks. They found that people perform substantially worse on stacked bar charts than on aligned bar charts, and that comparisons between adjacent bars are more accurate than between widely separated bars. However, the study did not explore why these differences occur. In this paper, we describe a series of follow-up experiments to further explore and explain their results. While our results generally confirm Cleveland & McGill's ranking of various bar chart configurations, we provide additional insight into the bar chart reading task and the sources of participants' errors. We use our results to propose new hypotheses on the perception of bar charts.

  7. Observation of B+ --> K0bar K+ and B0 --> K0 K0bar

    CERN Document Server

    Aubert, B; Boutigny, D; Couderc, F; Karyotakis, Yu; Lees, J P; Poireau, V; Tisserand, V; Zghiche, A; Graugès-Pous, E; Palano, A; Chen, J C; Qi, N D; Rong, G; Wang, P; Zhu, Y S; Eigen, G; Ofte, I; Stugu, B; Abrams, G S; Battaglia, M; Brown, D N; Button-Shafer, J; Cahn, R N; Charles, E; Gill, M S; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Mir, L M; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Wenzel, W A; Del, P; Amo Sanchez; Barrett, M; Ford, K E; Harrison, T J; Hart, A J; Hawkes, C M; Watson, A T; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Peters, K; Schröder, T; Steinke, M; Boyd, J T; Burke, J P; Cottingham, W N; Walker, D; Asgeirsson, D J; Çuhadar-Dönszelmann, T; Fulsom, B G; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Khan, A; Kyberd, P; Saleem, M; Sherwood, D J; Teodorescu, L; Blinov, V E; Bukin, A D; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Bruinsma, M; Chao, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M A; Mommsen, R K; Röthel, W; Stoker, D P; Abachi, S; Buchanan, C; Foulkes, S D; Gary, J W; Long, O; Shen, B C; Wang, K; Zhang, L; Hadavand, H K; Hill, E J; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Kovalskyi, D; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Nesom, G; Schalk, T; Schumm, B A; Seiden, A; Spradlin, P; Williams, D C; Wilson, M G; Albert, J; Chen, E; Dvoretskii, A; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Ryd, A; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Olivas, A; Ruddick, W O; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Chen, A; Eckhart, E A; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Zeng, Q; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Merkel, J; Petzold, A; Spaan, B; Brandt, T; Klose, V; Lacker, H M; Mader, W F; Nogowski, R; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Thiebaux, C; Verderi, M; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Robertson, A I; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cibinetto, G; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Prencipe, E; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo, M; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Brandenburg, G; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bard, D J; Bhimji, W; Bowerman, D A; Dauncey, P D; Egede, U; Flack, R L; Nash, J A; Nikolich, M B; Panduro-Vazquez, W; Behera, P K; Chai, X; Charles, M J; Mallik, U; Meyer, N T; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gritsan, A V; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Davier, M; Grosdidier, G; Höcker, A; Le Diberder, F R; Lepeltier, V; Lutz, A M; Oyanguren, A; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Stocchi, A; Wang, W F; Wormser, G; Cheng, C H; Lange, D J; Wright, D M; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; George, K A; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; Di Lodovico, F; Menges, W; Sacco, R; Cowan, G; Flächer, H U; Hopkins, D A; Jackson, P S; McMahon, T R; Ricciardi, S; Salvatore, F; Wren, A C; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; Naisbit, M T; Williams, J C; Yi, J I; Chen, C; Hulsbergen, W D; Jawahery, A; Lae, C K; Roberts, D A; Simi, G; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Saremi, S; Stängle, H; Cowan, R; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Kim, H; Mclachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; Cavallo, N; De Nardo, Gallieno; Fabozzi, F; Gatto, C; Lista, L; Monorchio, D; Paolucci, P; Piccolo, D; Sciacca, C; Baak, M A; Raven, G; Snoek, H L; Jessop, C P; LoSecco, J M; Allmendinger, T; Benelli, G; Corwin, L A; Gan, K K; Honscheid, K; Hufnagel, D; Jackson, P D; Kagan, H; Kass, R; Rahimi, A M; Regensburger, J J; Ter-Antonian, R; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Benayoun, M; Briand, H; Chauveau, J; David, P; Del, L; Buono; La Vaissière, C de; Hamon, O; Hartfiel, B L; Leruste, P; Malcles, J; Ocariz, J; Roos, L; Therin, G; Gladney, L; Biasini, M; Covarelli, R; Angelini, C; Batignani, G; Bettarini, S; Bucci, F; Calderini, G; Carpinelli, M; Cenci, R; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Mazur, M A; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J J; Haire, M; Judd, D; Wagoner, D E; Biesiada, J; Danielson, N; Elmer, P; Lau, Y P; Lü, C; Olsen, J; Smith, A J S; Telnov, A V; Bellini, F; Cavoto, G; D'Orazio, A; Del Re, D; Di Marco, E; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Safai-Tehrani, F; Voena, C; Ebert, M; Schröder, H; Waldi, R; Adye, T; De Groot, N; Franek, B; Olaiya, E O; Wilson, F F; Aleksan, R; Emery, S; Gaidot, A; Ganzhur, S F; Hamel de Monchenault, G; Kozanecki, Witold; Legendre, M; Vasseur, G; Yéche, C; Zito, M; Chen, X R; Liu, H; Park, W; Purohit, M V; Wilson, J R; Allen, M T; Aston, D; Bartoldus, R; Bechtle, P; Berger, N; Claus, R; Coleman, J P; Convery, M R; Cristinziani, M; Dingfelder, J C; Dorfan, J; Dubois-Felsmann, G P; Dujmic, D; Dunwoodie, W M; Field, R C; Glanzman, T; Gowdy, S J; Graham, M T; Grenier, P; Halyo, V; Hast, C; Hrynóva, T; Innes, W R; Kelsey, M H; Kim, P; Leith, D W G S; Li, S; Luitz, S; Lüth, V; Lynch, H L; MacFarlane, D B; Marsiske, H; Messner, R; Müller, D R; O'Grady, C P; Ozcan, V E; Perazzo, A; Perl, M; Pulliam, T; Ratcliff, B N; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Snyder, A; Stelzer, J; Su, D; Sullivan, M K; Suzuki, K; Swain, S K; Thompson, J M; Vavra, J; Van Bakel, N; Weaver, M; Weinstein, A J R; Wisniewski, W J; Wittgen, M; Wright, D H; Yarritu, A K; Yi, K; Young, C C; Burchat, P R; Edwards, A J; Majewski, S A; Petersen, B A; Roat, C; Wilden, L; Ahmed, S; Alam, M S; Bula, R; Ernst, J A; Jain, V; Pan, B; Saeed, M A; Wappler, F R; Zain, S B; Bugg, W; Krishnamurthy, M; Spanier, S M; Eckmann, R; Ritchie, J L; Satpathy, A; Schilling, C J; Schwitters, R F; Izen, J M; Lou, X C; Ye, S; Bianchi, F; Gallo, F; Gamba, D; Bomben, M; Bosisio, L; Cartaro, C; Cossutti, F; Della Ricca, G; Dittongo, S; Lanceri, L; Vitale, L; Azzolini, V; Lopez-March, N; Martínez-Vidal, F; Banerjee, Sw; Bhuyan, B; Brown, C M; Fortin, D; Hamano, K; Kowalewski, R V; Nugent, I M; Roney, J M; Sobie, R J; Back, J J; Harrison, P F; Latham, T E; Mohanty, G B; Pappagallo, M; Band, H R; Chen, X; Cheng, B; Dasu, S; Datta, M; Flood, K T; Hollar, J J; Kutter, P E; Mellado, B; Mihályi, A; Pan, Y; Pierini, M; Prepost, R; Wu, S L; Yu, Z; Neal, H

    2006-01-01

    We report observations of the b --> d penguin-dominated decays B+ --> K0bar K+ and B0 --> K0 K0bar in approximately 350 million Upsilon(4S) --> BBbar decays collected with the BaBar detector. We measure the branching fractions B(B+ --> K0bar K+) = (1.61 +/- 0.44 +/- 0.09) * 10^-6 and B(B0 --> K0 K0bar) = (1.08 +/- 0.28 +/- 0.11) * 10^-6, and the CP-violating charge asymmetry A_{CP}(K0bar K+) = 0.10 +/- 0.26 +/- 0.03. Using a vertexing technique previously employed in several analyses of all-neutral final states containing kaons, we report the first measurement of time-dependent CP-violating asymmetries in B0 --> K0 K0bar, obtaining S = -1.28 +0.80/-0.73 +0.11/-0.16 and C = -0.40 +/- 0.41 +/- 0.06. We also report improved measurements of the branching fraction B(B0 --> K0 pi+) = (23.9 +/- 1.1 +/- 1.0) * 10^-6 and CP-violating charge asymmetry A_{CP}(K0 pi+) = -0.029 +/- 0.039 +/- 0.010.

  8. Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus: Third Evaluation Report and Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K.; Eudy, L.

    2010-01-01

    This report describes operations at Connecticut Transit (CTTRANSIT) in Hartford for one prototype fuel cell bus and three new diesel buses operating from the same location. The prototype fuel cell bus was manufactured by Van Hool and ISE Corp. and features an electric hybrid drive system with a UTC Power PureMotion 120 Fuel Cell Power System and ZEBRA batteries for energy storage. The fuel cell bus started operation in April 2007, and evaluation results through October 2009 are provided in this report.

  9. Single Axis Attitude Control and DC Bus Regulation with Two Flywheels

    Science.gov (United States)

    Kascak, Peter E.; Jansen, Ralph H.; Kenny, Barbara; Dever, Timothy P.

    2002-01-01

    A computer simulation of a flywheel energy storage single axis attitude control system is described. The simulation models hardware which will be experimentally tested in the future. This hardware consists of two counter rotating flywheels mounted to an air table. The air table allows one axis of rotational motion. An inertia DC bus coordinator is set forth that allows the two control problems, bus regulation and attitude control, to be separated. Simulation results are presented with a previously derived flywheel bus regulator and a simple PID attitude controller.

  10. From Cryptic Toward Canonical Pre-mRNA Splicing in Pompe Disease: a Pipeline for the Development of Antisense Oligonucleotides

    Directory of Open Access Journals (Sweden)

    Atze J Bergsma

    2016-01-01

    Full Text Available While 9% of human pathogenic variants have an established effect on pre-mRNA splicing, it is suspected that an additional 20% of otherwise classified variants also affect splicing. Aberrant splicing includes disruption of splice sites or regulatory elements, or creation or strengthening of cryptic splice sites. For the majority of variants, it is poorly understood to what extent and how these may affect splicing. We have identified cryptic splicing in an unbiased manner. Three types of cryptic splicing were analyzed in the context of pathogenic variants in the acid α-glucosidase gene causing Pompe disease. These involved newly formed deep intronic or exonic cryptic splice sites, and a natural cryptic splice that was utilized due to weakening of a canonical splice site. Antisense oligonucleotides that targeted the identified cryptic splice sites repressed cryptic splicing at the expense of canonical splicing in all three cases, as shown by reverse-transcriptase-quantitative polymerase chain reaction analysis and by enhancement of acid α-glucosidase enzymatic activity. This argues for a competition model for available splice sites, including intact or weakened canonical sites and natural or newly formed cryptic sites. The pipeline described here can detect cryptic splicing and correct canonical splicing using antisense oligonucleotides to restore the gene defect.

  11. A Rule-Based Energy Management Strategy for a Plug-in Hybrid School Bus Based on a Controller Area Network Bus

    Directory of Open Access Journals (Sweden)

    Jiankun Peng

    2015-06-01

    Full Text Available This paper presents a rule-based energy management strategy for a plug-in hybrid school bus (PHSB. In order to verify the effectiveness and rationality of the proposed energy management strategy, the powertrain and control models were built with MATLAB/Simulink. The PHSB powertrain model includes an engine model, ISG (integrated started and generator model, drive motor model, power battery packs model, driver model, and vehicle longitudinal dynamics model. To evaluate the controller area network (CAN bus performance features such as the bus load, signal hysteresis, and to verify the reliability and real-time performance of the CAN bus multi-node control method, a co-simulation platform was built with CANoe and MATLAB/Simulink. The co-simulation results show that the control strategy can meet the requirements of the PHSB’s dynamic performance. Meanwhile, the charge-depleting mode (CD and charge-sustaining mode (CS can switch between each other and maintain a state-of-charge (SoC of around 30%, indicating that the energy management strategy effectively extends the working period of the CD mode and improves the fuel economy further. The energy consumption per 100 km includes 13.7 L diesel and 10.5 kW·h electricity with an initial SoC of 75%. The CANoe simulation results show that the bus communication performs well without error frames.

  12. Barred galaxy formation in the EAGLE cosmological simulation

    Science.gov (United States)

    Abadi, M. G.; Algorry, D. G.

    2017-07-01

    We present results about the formation and evolution of stellar bars in Milky-Way sized galaxies using the EAGLE ΛCDM cosmological hydrodynamical simulation. In agreement with observational results, this simulation shows that ˜ 40% of our simulated disk galaxies have a stellar bar with a wide variety of bar strengths. Typical bar lengths are ˜ 6.5 kpc also comparing favourably to observed ones. Our unbarred disks are more gas-rich and star-forming than those having a strong bar. In concordance with previous work, bars develop in galaxies where the disk is gravitationally dominant over the dark matter halo.

  13. Measurement of the Diameters of Deformed Bars in Concrete Using an Electromagnetic Wave Radar (in the Presence of Cross Bars)

    Science.gov (United States)

    Tanaka, Shogo; Begum, Halima

    The authors previously proposed a method to measure the diameter of the deformed reinforcing bars in concrete structures nondestructively using an electromagnetic wave radar. The method estimates the periodicity of the knots of the inspected bar and utilizes the standard relationship between the knot's pitch and the diameter of the bar to measure the diameter indirectly. The effectiveness of the method was verified using test specimens where the bars were placed parallel to each other. However, in practical case, where other reinforcing bars cross the inspected bar perpendicularly, the stronger reflections from the cross bars influence the reflection from the inspected bar. This paper thus proposes a general method which eliminates such unwanted influences from the cross bars and measures the diameter accurately even in practical environment.

  14. Measurement of the forward-backward asymmetry of $\\Lambda$ and $\\bar{\\Lambda}$ production in $p \\bar{p}$ collisions

    CERN Document Server

    Abazov, Victor Mukhamedovich; Acharya, Bannanje Sripath; Adams, Mark Raymond; Adams, Todd; Agnew, James P; Alexeev, Guennadi D; Alkhazov, Georgiy D; Alton, Andrew K; Askew, Andrew Warren; Atkins, Scott; Augsten, Kamil; Avila, Carlos A; Badaud, Frederique; Bagby, Linda F; Baldin, Boris; Bandurin, Dmitry V; Banerjee, Sunanda; Barberis, Emanuela; Baringer, Philip S; Bartlett, JFrederick; Bassler, Ursula Rita; Bazterra, Victor; Bean, Alice L; Begalli, Marcia; Bellantoni, Leo; Beri, Suman B; Bernardi, Gregorio; Bernhard, Ralf Patrick; Bertram, Iain A; Besancon, Marc; Beuselinck, Raymond; Bhat, Pushpalatha C; Bhatia, Sudeep; Bhatnagar, Vipin; Blazey, Gerald Charles; Blessing, Susan K; Bloom, Kenneth A; Boehnlein, Amber S; Boline, Daniel Dooley; Boos, Edward E; Borissov, Guennadi; Borysova, Maryna; Brandt, Andrew; Brandt, Oleg; Brock, Raymond L; Bross, Alan D; Brown, Duncan Paul; Bu, Xue-Bing; Buehler, Marc; Buescher, Volker; Bunichev, Viacheslav Yevgenyevich; Burdin, Sergey; Buszello, Claus Peter; Camacho-Perez, Enrique; Casey, Brendan Cameron Kieran; Castilla-Valdez, Heriberto; Caughron, Seth Aaron; Chakrabarti, Subhendu; Chan, Kwok Ming Leo; Chandra, Avdhesh; Chapon, Emilien; Chen, Guo; Cho, Sung-Woong; Choi, Suyong; Choudhary, Brajesh C; Cihangir, Selcuk; Claes, Daniel R; Clutter, Justace Randall; Cooke, Michael P; Cooper, William Edward; Corcoran, Marjorie D; Couderc, Fabrice; Cousinou, Marie-Claude; Cuth, Jakub; Cutts, David; Das, Amitabha; Davies, Gavin John; de Jong, Sijbrand Jan; De La Cruz-Burelo, Eduard; Deliot, Frederic; Demina, Regina; Denisov, Dmitri S; Denisov, Sergei P; Desai, Satish Vijay; Deterre, Cecile; DeVaughan, Kayle Otis; Diehl, HThomas; Diesburg, Michael; Ding, Pengfei; Dominguez, DAaron M; Dubey, Abhinav Kumar; Dudko, Lev V; Duperrin, Arnaud; Dutt, Suneel; Eads, Michael T; Edmunds, Daniel L; Ellison, John A; Elvira, VDaniel; Enari, Yuji; Evans, Harold G; Evdokimov, Anatoly V; Evdokimov, Valeri N; Faure, Alexandre; Feng, Lei; Ferbel, Thomas; Fiedler, Frank; Filthaut, Frank; Fisher, Wade Cameron; Fisk, HEugene; Fortner, Michael R; Fox, Harald; Franc, Jiri; Fuess, Stuart C; Garbincius, Peter H; Garcia-Bellido, Aran; Garcia-Gonzalez, Jose Andres; Gavrilov, Vladimir B; Geng, Weigang; Gerber, Cecilia Elena; Gershtein, Yuri S; Ginther, George E; Gogota, Olga; Golovanov, Georgy Anatolievich; Grannis, Paul D; Greder, Sebastien; Greenlee, Herbert B; Grenier, Gerald Jean; Gris, Phillipe Luc; Grivaz, Jean-Francois; Grohsjean, Alexander; Gruenendahl, Stefan; Gruenewald, Martin Werner; Guillemin, Thibault; Gutierrez, Gaston R; Gutierrez, Phillip; Haley, Joseph Glenn Biddle; Han, Liang; Harder, Kristian; Harel, Amnon; Hauptman, John Michael; Hays, Jonathan M; Head, Tim; Hebbeker, Thomas; Hedin, David R; Hegab, Hatim; Heinson, Ann; Heintz, Ulrich; Hensel, Carsten; Heredia-De La Cruz, Ivan; Herner, Kenneth Richard; Hesketh, Gavin G; Hildreth, Michael D; Hirosky, Robert James; Hoang, Trang; Hobbs, John D; Hoeneisen, Bruce; Hogan, Julie; Hohlfeld, Mark; Holzbauer, Jenny Lyn; Howley, Ian James; Hubacek, Zdenek; Hynek, Vlastislav; Iashvili, Ia; Ilchenko, Yuriy; Illingworth, Robert A; Ito, Albert S; Jabeen, Shabnam; Jaffre, Michel J; Jayasinghe, Ayesh; Jeong, Min-Soo; Jesik, Richard L; Jiang, Peng; Johns, Kenneth Arthur; Johnson, Emily; Johnson, Marvin E; Jonckheere, Alan M; Jonsson, Per Martin; Joshi, Jyoti; Jung, Andreas Werner; Juste, Aurelio; Kajfasz, Eric; Karmanov, Dmitriy Y; Katsanos, Ioannis; Kaur, Manbir; Kehoe, Robert Leo Patrick; Kermiche, Smain; Khalatyan, Norayr; Khanov, Alexander; Kharchilava, Avto; Kharzheev, Yuri N; Kiselevich, Ivan Lvovich; Kohli, Jatinder M; Kozelov, Alexander V; Kraus, James Alexander; Kumar, Ashish; Kupco, Alexander; Kurca, Tibor; Kuzmin, Valentin Alexandrovich; Lammers, Sabine Wedam; Lebrun, Patrice; Lee, Hyeon-Seung; Lee, Seh-Wook; Lee, William M; Lei, Xiaowen; Lellouch, Jeremie; Li, Dikai; Li, Hengne; Li, Liang; Li, Qi-Zhong; Lim, Jeong Ku; Lincoln, Donald W; Linnemann, James Thomas; Lipaev, Vladimir V; Lipton, Ronald J; Liu, Huanzhao; Liu, Yanwen; Lobodenko, Alexandre; Lokajicek, Milos; Lopes de Sa, Rafael; Luna-Garcia, Rene; Lyon, Adam Leonard; Maciel, Arthur KA; Madar, Romain; Magana-Villalba, Ricardo; Malik, Sudhir; Malyshev, Vladimir L; Mansour, Jason; Martinez-Ortega, Jorge; McCarthy, Robert L; Mcgivern, Carrie Lynne; Meijer, Melvin M; Melnitchouk, Alexander S; Menezes, Diego D; Mercadante, Pedro Galli; Merkin, Mikhail M; Meyer, Arnd; Meyer, Jorg Manfred; Miconi, Florian; Mondal, Naba K; Mulhearn, Michael James; Nagy, Elemer; Narain, Meenakshi; Nayyar, Ruchika; Neal, Homer A; Negret, Juan Pablo; Neustroev, Petr V; Nguyen, Huong Thi; Nunnemann, Thomas P; Hernandez Orduna, Jose de Jesus; Osman, Nicolas Ahmed; Osta, Jyotsna; Pal, Arnab; Parashar, Neeti; Parihar, Vivek; Park, Sung Keun; Partridge, Richard A; Parua, Nirmalya; Patwa, Abid; Penning, Bjoern; Perfilov, Maxim Anatolyevich; Peters, Reinhild Yvonne Fatima; Petridis, Konstantinos; Petrillo, Gianluca; Petroff, Pierre; Pleier, Marc-Andre; Podstavkov, Vladimir M; Popov, Alexey V; Prewitt, Michelle; Price, Darren; Prokopenko, Nikolay N; Qian, Jianming; Quadt, Arnulf; Quinn, Gene Breese; Ratoff, Peter N; Razumov, Ivan A; Ripp-Baudot, Isabelle; Rizatdinova, Flera; Rominsky, Mandy Kathleen; Ross, Anthony; Royon, Christophe; Rubinov, Paul Michael; Ruchti, Randal C; Sajot, Gerard; Sanchez-Hernandez, Alberto; Sanders, Michiel P; Santos, Angelo Souza; Savage, David G; Savitskyi, Mykola; Sawyer, HLee; Scanlon, Timothy P; Schamberger, RDean; Scheglov, Yury A; Schellman, Heidi M; Schott, Matthias; Schwanenberger, Christian; Schwienhorst, Reinhard H; Sekaric, Jadranka; Severini, Horst; Shabalina, Elizaveta K; Shary, Viacheslav V; Shaw, Savanna; Shchukin, Andrey A; Simak, Vladislav J; Skubic, Patrick Louis; Slattery, Paul F; Smirnov, Dmitri V; Snow, Gregory R; Snow, Joel Mark; Snyder, Scott Stuart; Soldner-Rembold, Stefan; Sonnenschein, Lars; Soustruznik, Karel; Stark, Jan; Stoyanova, Dina A; Strauss, Michael G; Suter, Louise; Svoisky, Peter V; Titov, Maxim; Tokmenin, Valeriy V; Tsai, Yun-Tse; Tsybychev, Dmitri; Tuchming, Boris; Tully, Christopher George T; Uvarov, Lev; Uvarov, Sergey L; Uzunyan, Sergey A; Van Kooten, Richard J; van Leeuwen, Willem M; Varelas, Nikos; Varnes, Erich W; Vasilyev, Igor A; Verkheev, Alexander Yurievich; Vertogradov, Leonid S; Verzocchi, Marco; Vesterinen, Mika; Vilanova, Didier; Vokac, Petr; Wahl, Horst D; Wang, Michael HLS; Warchol, Jadwiga; Watts, Gordon Thomas; Wayne, Mitchell R; Weichert, Jonas; Welty-Rieger, Leah Christine; Williams, Mark Richard James; Wilson, Graham Wallace; Wobisch, Markus; Wood, Darien Robert; Wyatt, Terence R; Xie, Yunhe; Yamada, Ryuji; Yang, Siqi; Yasuda, Takahiro; Yatsunenko, Yuriy A; Ye, Wanyu; Ye, Zhenyu; Yin, Hang; Yip, Kin; Youn, Sungwoo; Yu, Jiaming; Zennamo, Joseph; Zhao, Tianqi Gilbert; Zhou, Bing; Zhu, Junjie; Zielinski, Marek; Zieminska, Daria; Zivkovic, Lidija

    2016-01-01

    We study $\\Lambda$ and $\\bar{\\Lambda}$ production asymmetries in $p \\bar{p} \\rightarrow \\Lambda (\\bar{\\Lambda}) X$, $p \\bar{p} \\rightarrow J/\\psi \\Lambda (\\bar{\\Lambda}) X$, and $p \\bar{p} \\rightarrow \\mu^\\pm \\Lambda (\\bar{\\Lambda}) X$ events recorded by the D0 detector at the Fermilab Tevatron collider at $\\sqrt{s} = 1.96$ TeV. We find an excess of $\\Lambda$'s ($\\bar{\\Lambda}$'s) produced in the proton (antiproton) direction. This forward-backward asymmetry is measured as a function of rapidity. We confirm that the $\\bar{\\Lambda}/\\Lambda$ production ratio, measured by several experiments with various targets and a wide range of energies, is a universal function of "rapidity loss", i.e., the rapidity difference of the beam proton and the lambda.

  15. Rules and tools to predict the splicing effects of exonic and intronic mutations.

    Science.gov (United States)

    Ohno, Kinji; Takeda, Jun-Ichi; Masuda, Akio

    2017-09-26

    Development of next generation sequencing technologies has enabled detection of extensive arrays of germline and somatic single nucleotide variations (SNVs) in human diseases. SNVs affecting intronic GT-AG dinucleotides invariably compromise pre-mRNA splicing. Most exonic SNVs introduce missense/nonsense codons, but some affect auxiliary splicing cis-elements or generate cryptic GT-AG dinucleotides. Similarly, most intronic SNVs are silent, but some affect canonical and auxiliary splicing cis-elements or generate cryptic GT-AG dinucleotides. However, prediction of the splicing effects of SNVs is challenging. The splicing effects of SNVs generating cryptic AG or disrupting canonical AG can be inferred from the AG-scanning model. Similarly, the splicing effects of SNVs affecting the first nucleotide G of an exon can be inferred from AG-dependence of the 3' splice site (ss). A variety of tools have been developed for predicting the splicing effects of SNVs affecting the 5' ss, as well as exonic and intronic splicing enhancers/silencers. In contrast, only two tools, the Human Splicing Finder and the SVM-BP finder, are available for predicting the position of the branch point sequence. Similarly, IntSplice and Splicing based Analysis of Variants (SPANR) are the only tools to predict the splicing effects of intronic SNVs. The rules and tools introduced in this review are mostly based on observations of a limited number of genes, and no rule or tool can ensure 100% accuracy. Experimental validation is always required before any clinically relevant conclusions are drawn. Development of efficient tools to predict aberrant splicing, however, will facilitate our understanding of splicing pathomechanisms in human diseases. For further resources related to this article, please visit the WIREs website. © 2017 Wiley Periodicals, Inc.

  16. Star formation history of barred disc galaxies

    NARCIS (Netherlands)

    Sanchez-Blazquez, P.; Ocvirk, P.; Gibson, B. K.; Perez, I.; Peletier, R. F.

    We present the first results of a pilot study aimed at understanding the influence of bars on the evolution of galaxy discs through the study of their stellar content. We examine here the kinematics, star formation history, mass-weighted, luminosity-weighted and single stellar population (SSP)

  17. BaBar Explores CP Violation

    Energy Technology Data Exchange (ETDEWEB)

    Karyotakis, Jean Yannis

    2003-05-16

    The most recent results obtained by the BABAR experiment at the PEP-II asymmetric-energy B Factory at SLAC on CP-violating asymmetries and branching fractions for neutral and charged B decays are presented here. The analysis was performed on a data sample of {approx} 88 million {Upsilon}(4S) {yields} B{bar B} decays collected between 1999 and 2002. Using b {yields} c{bar c}s decays, we measure sin2{beta} = 0.741 {+-} 0.067(stat) {+-} 0.034(syst). We also present sin2{beta} measurements from, b {yields} s{bar s}s and b {yields} c{bar c}d processes. From neutral B meson decays to two-body final states of charged pions and kaons, we derive for the CP violating parameters, S{sub {pi}{pi}} = 0.02 {+-} 0.34 {+-} 0.05 [-0.54, +0.58] and C{sub {pi}{pi}} = -0.30 {+-} 0.25 {+-} 0.04 [-0.72, +0.12]. First results for B {yields} {pi}{sup +}{pi}{sup -}{pi}{sup 0} and K{sup {+-}}{pi}{sup {-+}}{pi}{sup 0} final states dominated by the {rho}{sup {+-}} resonance, are also presented.

  18. Should an infected Nuss bar be removed?

    NARCIS (Netherlands)

    Van Renterghem, KM; von Bismarck, S; Bax, NMA; Fleer, A; Hollwarth, ME

    Background: The Nuss procedure is a minimally invasive procedure for correction of pectus excavatum. It involves insertion of a substernal metal bar. A feared complication of any implanted device is infection, which often necessitates removal. The purpose of this report is to describe the authors'

  19. The BaBar Data Acquisition System

    CERN Document Server

    Scott, I; Grosso, P; Huffer, M E; O'Grady, C; Russell, J J

    1999-01-01

    The BaBar experiment at the Stanford Linear Accelerator Center is designed to perform a search for CP violation by ana-lyzing the decays of a very large sample of B and B(Bar) mesons produced at the high luminosity PEP-II accelerator. The data acquisition system must cope with a sustained high event rate, while supporting real time feature extraction and data compression with minimal dead time. The BaBar data acquisition system is based around a common VME interface to the electronics read-out of the separate detec-tor subsystems. Data from the front end electronics is read into commercial VME processors via a custom "Personality Card" and PCI interface. The commercial CPUs run the Tornado operating system to provide a platform for detector subsystem code to perform the necessary data processing. The data is read out via a non-blocking network switch to a farm of commercial UNIX processors. The current implementation of the BaBar data acquisition sys-tem has been shown to sustain a Level 1 trigger rate of 1.3...

  20. Adaptive Virtual Tow Bar, research results 2016

    NARCIS (Netherlands)

    Willemsen, D.M.C.; Hueting, T.F.; Joosten, B.; Uittenbogaard, J.; Martens, M.H.

    2017-01-01

    This document reports the advances made in 2016 for the Early Research Program (ERP) Human Enhancement: Adaptive Automation, sub-project Adaptive Virtual Tow Bar. The ambition of the large scale TNO Early Research Program (ERP) Human Enhancement is to develop a transparent (human-in-the-loop)