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Sample records for bus bar splices

  1. The RRR of the Cu components of the LHC main bus bar splices

    CERN Document Server

    Heck, S; Fessia, P; Principe, R; CERN. Geneva. TE Department

    2010-01-01

    The different LHC main bus bar splice components (bus bar cable, splice U-piece and wedge and bus bar stabilizer) are subjected to different heat treatments (HTs) during bus bar assembly and splice soldering. The influence of soldering HTs on the RRR of the LHC main bus bar cable strands has been determined. The RRR of several splice U-pieces and wedges dismounted from the LHC has been measured. A correlation between the Vickers hardness and the RRR of the high purity Cu profiles has been established. All U-pieces tested that were produced before 2009 have a RRR>200, while the RRR of all wedges and of U-pieces of 2009 production have a much lower RRR. All tests of LHC main bus bar samples performed so far in the laboratory indicate a RRR of approximately 200 or higher.

  2. Resistance of LHC main bus bar splices at room temperature and at 77.4 K

    CERN Document Server

    Heck, S; Scheuerlein, Chr; CERN. Geneva. TE Department

    2009-01-01

    As part of the quality control the resistance of newly produced LHC main bus bar splices is now routinely measured at room temperature (RT) in order to conclude on the electrical continuity of the bus bar stabiliser across the splice under operating conditions. In this note we present splice resistance measurements that have been performed at RT and in liquid nitrogen (LN) in the CERN Cryolab with “ideal” splices (represented by continuous dipole and quadrupole bus bars), and with dipole and quadrupole splices with different defects, which cause an additional RT splice resistance of up to 60 µΩ. The RT resistance (RRT) results obtained with the Cryolab set-up are compared to the calculated resistance values and with the so-called R-8 and R-16 resistance results, as they are measured in the LHC tunnel with a Digital Low Resistance Ohmmeter with a voltage tap distance of 8 cm or 16 cm. The RT to LN resistance ratio has been determined for all splices in order to study the influence of the resistance of th...

  3. Hiding Solar-Array Bus Bars

    Science.gov (United States)

    Hufnagel, W. F.

    1983-01-01

    End terminals mounted under cells, maximizing usable illuminated area. Reconfigured solar panel bus bars placed under cells, reducing portion of module area not occupied by active silicon. Underside of last cell in string of cells serves as contact for positive bus. Negative tab of last cell in string is wrapped around from top of cell. Tabs are connected to output boards mounted under cells.

  4. Heat Transfer in the LHC Main Superconducting Bus Bars

    CERN Document Server

    Granieri, P P; Richter, D

    2011-01-01

    CERN is performing a systematic analysis of the interconnecting bus bars of the Large Hadron Collider (LHC) main magnets. Their thermal, electrical, mechanical and hydraulic performances are addressed. In the frame of these studies, the heat transfer between the main superconducting (SC) bus bars and the surrounding He bath is investigated. It represents a key parameter in the comprehension of the bus bars stability and quench propagation mechanisms, thus crucial for the analysis of the 2008 incident which was triggered by a defective bus bars joint. This paper reports on the experimental tests and relative analysis aiming at describing the thermal behavior of the LHC main bus bars.

  5. A train for the bus(bars)

    CERN Multimedia

    CERN Bulletin

    2013-01-01

    On 8 April, the first SMACC (Superconducting Magnets and Circuits Consolidation) teams began work in the LHC tunnel. They are responsible for opening the interconnects between the magnets, laying the groundwork for the series of operations that will be performed by the team riding the ‘consolidation train’.   A technician installs the machine tool that allows them to prepare the surface of the section of the bar where the shunt will be fixed. The LHC’s 1,670 dipoles and quadrupoles are powered by power converters and connected by copper 'busbars’. The superconducting cables run through these bars, carrying a current of up to 11,850 amps. Six superconducting cables meet at each interconnect, where they are held together by a soldered (see box) electrical connection sandwiched between two pieces of copper, forming the splice between the busbars of the neighbouring magnets. The integrity of the electrical circuit is depen...

  6. Bus bar electrical feedthrough for electrorefiner system

    Science.gov (United States)

    Williamson, Mark; Wiedmeyer, Stanley G; Willit, James L; Barnes, Laurel A; Blaskovitz, Robert J

    2013-12-03

    A bus bar electrical feedthrough for an electrorefiner system may include a retaining plate, electrical isolator, and/or contact block. The retaining plate may include a central opening. The electrical isolator may include a top portion, a base portion, and a slot extending through the top and base portions. The top portion of the electrical isolator may be configured to extend through the central opening of the retaining plate. The contact block may include an upper section, a lower section, and a ridge separating the upper and lower sections. The upper section of the contact block may be configured to extend through the slot of the electrical isolator and the central opening of the retaining plate. Accordingly, relatively high electrical currents may be transferred into a glovebox or hot-cell facility at a relatively low cost and higher amperage capacity without sacrificing atmosphere integrity.

  7. Black silicon solar cells with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by maskless reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Four different methods to obtain blackened bus-bar strings were compared with respect to reflectance, and two of these methods (i.e., oxidized copper and etched solder) were used to fabricate functional allblack solar 9-cell panels. The black bus-bars (e.......g., by oxidized copper) have a reflectance below 3% in the entire visible wavelength range. The combination of black silicon cells and blackened bus-bars results in aesthetic, all-black panels based on conventional, front-contacted solar cells without compromising efficiency....

  8. BUS 1000母线保护的应用%Application of BUS 1000 Bus-Bar Protection

    Institute of Scientific and Technical Information of China (English)

    马晓光; 张贵金; 冷雪玉

    2001-01-01

    发电厂的母线是电厂中的一个重要组成元件,当母线上发生故障时,将使连接在故障母线上的所有元件在修复故障母线期间,或转换到另一组母线上运行以前遭到停电。同时还可能破坏系统稳定,造成严重后果。因此,母线保护装置是正确迅速切除母线故障的重要装置,它的拒动或误动将给电力系统带来严重危害。详细介绍了华能丹东发电厂350 MW发电机组采用的BUS 1000型母线保护的原理,并对其正常运行及区内、外故障、断路器失灵保护、母联断路器过流保护等几个方面作了详细分析。%Bus bar is an important component in e lectric power plant.When bus bar fault occurs,all components connected on the fa ulty bus bar will be in outage until power restores by repairing the faulty bus bar or switching them over to another bus bar.Furthermore,it may cause the destr uction of system stability resulting in serious consequence.Bus bar protection d evice is the very device to quickly remove bus bar faults,and its malfunctioning will bring serious harm to power system. The principle of BUS 1000 bus bar prot ection used for 350 MW unit of dandong power plant is introduced.Its normal oper ation and several aspects,such as in-and out-area faults,out-of-order breake r protection,bus bar breaker over-current protection,are analyzed in detail.

  9. Black silicon with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io;

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by mask-less reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Black bus-bars were realized by oxidized copper resulting in reflectance below 3% in the entire visible wavelength range. The combination of these two technologies may result in aesthetic, all-black panels based on conventional, front-contacted solar cells...

  10. Mechanical analysis of the main bus bars in the DFBA shuffling modules

    CERN Document Server

    Ramos, D

    2012-01-01

    The main bus bars (13 kA) inside the shuffling modules of the distribution feed boxes powering the LHC arcs (DFBA) are subjected to high Lorentz forces. The structural behaviour of the bus bars under such forces is here analysed. The results are discussed with respect to a risk of structural failure due to excessive deformation or degradation of the electrical insulation by repeated contact with other surfaces.

  11. An analytical algorithm to determine allowable ampacities of horizontally installed rectangular bus bars

    Directory of Open Access Journals (Sweden)

    Klimenta Dardan O.

    2016-01-01

    Full Text Available The main objective of this paper is to propose an algorithm for the determination of the allowable ampacities of single rectangular-section bus bars without the occurrence of correction factors. Without correction factors, the ampacity computation of the copper and aluminium bus bars is fully automatized. The analytical algorithm has been implemented in a computer program code that along with the allowable ampacity can compute the bus bar temperature and the individual heat transfer coefficient for each side of the bus bar, as well as their corresponding power losses. Natural and forced convection correlations for rectangular bus bars are applied. Effects of the solar radiation and radiation heat losses from the bus bar surface are taken into consideration as well. The finite element method (FEM has been used for the linear/non-linear steady-state thermal analysis, i.e. for validation of the analytical algorithm. All FEM-based numerical computations were carried out using the COMSOL Heat Transfer Module. [Projekat Ministarstva nauke Republike Srbije, br. TR33046

  12. Development of multifunctional digital bus bar protection relay

    Energy Technology Data Exchange (ETDEWEB)

    Yuki, Masaru; Yoshikawa, Motoyasu; Hayashi, Eiji; Oki, Michio; Matsumoto, Yutaka; Iwaya, fumio; Yoshizumi, Tatunori (Kansai Electric Power Co. Inc., Osaka (Japan); Hitachi Ltd., Tokyo (Japan))

    1989-03-25

    The existing analog bus bar protection relays of a substation have been easily affected by saturation of the current transformer and apt to malfunction at an accident occurred outside. Kansai Electric Power Co. developed jointly with Hitachi Manufacturing Co. a new calculation algorithm to avert the effect of the current transformer saturation (ratio differential relay system with memory of the amount of inhibition) by digitizing the above existing relay. Taking notice of the fact that transient saturation of the current transformer occurs from at least one half wave or later after the accident current started passing, this system memorizes the amount of inhibition of the relay by a signal within the time zone of nonsaturation immediately after the start of the accident current and by this value prevents malfunction at the time of current transformer saturation. The systems to which this relay is applied are as follows: system voltages are 77kV and 154kV for the resistance grounded neutral system, and 275kV for the resistance grounded neutral system and 275kV for the system with solidly earthed neutral. The substations to which this relay is to be installed are medium and large scale substations each of which has 8 or more terminals. Its short circuit capacity is 3,300kVA for the 77kV system, 10,600kVA for the 154kV system and 23,800kVA for the 275kV system. 8 figs., 7 tabs.

  13. The high current bus-bars of the LHC from conception to manufacture

    CERN Document Server

    Belova, L; Perinet-Marquet, J-L; Urpin, C

    2006-01-01

    The main magnets of the LHC are series-connected electrically in different excitation circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper section all along length. The function of the copper section is essentially to provide an alternative path for the magnet current in case the superconducting cable loses its superconductivity and returns to normal state because of a transient system disturbance or normal zone propagation coming from the neighboring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100 ° C while the copper is conducting. With regard to that, a quench signal is initiated, which in turn triggers the ramping down of the current from 13000 A to 0....

  14. Calculation of Flexible Bus-Bars Electrodynamic Stability with Application of Implicit Scheme

    Directory of Open Access Journals (Sweden)

    Y. G. Panamarenka

    2008-01-01

    Full Text Available A numerical method for calculation of open-air substations’ flexible bus-bars dynamic at short-circuit has been improved on equations of a flexible elastic string with application of an implicit scheme. On the basis of the numerical method a computer program FLEBUS for calculation of substations’ flexible bus-bars dynamic at short-circuit has been developed. An approbation and an estimation of calculation result reliability have been carried out in accordance with the program while using experimental data. On the basis of the obtained information it is possible to assert that the developed program is an independent tool for calculation of electrodynamic stability of substations’ flexible bus-bars.

  15. Realization and optimization of bus bar current transducers based on Hall effect sensors

    Science.gov (United States)

    Blagojević, Marjan; Jovanović, Uglješa; Jovanović, Igor; Mančić, Dragan; Popović, Radivoje S.

    2016-06-01

    In this paper the realization and optimization of two coreless open-loop bus bar current transducers based on a Hall effect sensor are presented. Two types of bus bar are evaluated: flat rectangular and rectangular with a restrictive region in the middle. Both realized transducers are capable of measuring AC and DC currents up to 300 A and 10 kHz frequency with nonlinearity less than 0.3% in the entire range. Several methods for resolving issues with the skin effect and stray magnetic fields are presented along with the experimental test results. Some of the presented methods are novel and have never been evaluated.

  16. Design and Manufacture of the Superconducting Bus-bars for the LHC Main Magnets

    CERN Document Server

    Belova, L M; Perinet-Marquet, J L; Ivanov, P; Urpin, C

    2002-01-01

    The main magnets of the LHC are series-connected electrically in different powering circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper profile all along the length. The function of the copper profile is essentially to provide an alternative path for the current in case the superconducting cable loses its superconducting state and returns to normal state because of a transient disturbance or of a normal zone propagation coming from the neighbouring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100°C while the copper is conducting. When a resistive transition is detected, the protection systems triggers the ramping down of the current from 13000 A to 0. The ramp rate must not exceed a maximum value to avoid the transition of magnets series-connected in the circuit. This paper concerns th...

  17. Measurement of Parasitic Inductances in the Bus-Bar Assembly of a High Power Voltage Source Converter

    Science.gov (United States)

    Datta, Aniket; Narayanan, G.

    2016-12-01

    Insulated gate bipolar transistor (IGBT) based voltage source converters use copper plates with insulating sheets in between them (sandwich bus-bar arrangement) for connecting the different device terminals in the power circuit. In such converters, the parasitic inductances in the power circuit are crucial as they cause overvoltage spikes across the device. Also, the parasitics affect the current sharing between IGBTs when they are connected in parallel in high power converters. The conduction path through plates and fasteners in the bus-bar assembly is three-dimensional and quite complex, making analytical evaluation of the stray inductance quite challenging. The first objective here is to present a simple experimental setup and experimental procedure, which are convenient for power electronic engineers, to measure the bus-bar inductance. The next objective is to carry out experimental studies on the inductances offered by different components and sub-assemblies in a bus-bar assembly. This includes evaluation of inductances of the different conduction paths in typical bus-bar plates. The third objective is to experimentally evaluate the parasitic inductances in the bus-bar assembly of a commercial 250 kVA high power converter. Each leg of this converter consists of two 300 A/1200 V IGBTs connected in parallel. The effective inductance seen by the individual device modules are determined experimentally.

  18. Thermo-electric Analysis of the Interconnection of the LHC main Superconducting Bus Bars

    CERN Document Server

    Granieri, P P; Casali, M; Bottura, L; Siemko, A

    2013-01-01

    Spurred by the question of the maximum allowable energy for the operation of the Large Hadron Collider (LHC), we have progressed in the understanding of the thermo-electric behavior of the 13 kA superconducting bus bars interconnecting its main magnets. A deep insight of the underlying mechanisms is required to ensure the protection of the accelerator against undesired effects of resistive transitions. This is especially important in case of defective interconnections which can jeopardize the operation of the whole LHC. In this paper we present a numerical model of the interconnections between the main dipole and quadrupole magnets, validated against experimental tests of an interconnection sample with a purposely built-in defect. We consider defective interconnections featuring a lack of bonding among the superconducting cables and the copper stabilizer components, such as those that could be present in the machine. We evaluate the critical defect length limiting the maximum allowable current for powering th...

  19. Analysis of Defective Interconnections of the 13 kA LHC Superconducting Bus Bars

    CERN Document Server

    Granieri, P P; Bianchi, M; Breschi, M; Bottura, L; Willering, G

    2012-01-01

    The interconnections between Large Hadron Collider (LHC) main dipole and quadrupole magnets are made of soldered joints of two superconducting cables stabilized by a copper bus bar. The 2008 incident revealed the possible presence of defects in the interconnections of the 13 kA circuits that could lead to unprotected resistive transitions. Since then thorough experimental and numerical investigations were undertaken to determine the safe operating conditions for the LHC. This paper reports the analysis of experimental tests reproducing defective interconnections between main quadrupole magnets. A thermo-electromagnetic model was developed taking into account the complicated sample geometry. Close attention was paid to the physical description of the heat transfer towards helium, one of the main unknown parameters. The simulation results are reported in comparison with the measurements in case of static He I cooling bath. The outcome of this study constitutes a useful input to improve the stability assessment ...

  20. Thermo-electric analysis of the interconnection of the LHC main superconducting bus bars

    Science.gov (United States)

    Granieri, P. P.; Breschi, M.; Casali, M.; Bottura, L.; Siemko, A.

    2013-01-01

    Spurred by the question of the maximum allowable energy for the operation of the Large Hadron Collider (LHC), we have progressed in the understanding of the thermo-electric behavior of the 13 kA superconducting bus bars interconnecting its main magnets. A deep insight of the underlying mechanisms is required to ensure the protection of the accelerator against undesired effects of resistive transitions. This is especially important in case of defective interconnections which can jeopardize the operation of the whole LHC. In this paper we present a numerical model of the interconnections between the main dipole and quadrupole magnets, validated against experimental tests of an interconnection sample with a purposely built-in defect. We consider defective interconnections featuring a lack of bonding among the superconducting cables and the copper stabilizer components, such as those that could be present in the machine. We evaluate the critical defect length limiting the maximum allowable current for powering the magnets. We determine the dependence of the critical defect length on different parameters as the heat transfer towards the cooling helium bath, the quality of manufacturing, the operating conditions and the protection system parameters, and discuss the relevant mechanisms.

  1. High-Current Bus Splice Resistances and Implications for the Operating Energy of the LHC

    CERN Document Server

    Koratzinos, M; Charifoulline, Z; Dahlerup-Petersen, K; Denz, R; Flora, R H; Pfeffer, H; Scheuerlein, C; Schmidt, R; Siemko, A; Strait, J; Verweij, A

    2010-01-01

    At each interconnection between LHC main magnets a low-resistance solder joint must be made between superconducting cables in order to provide a continuous current path through the superconductor and also to the surrounding copper stabilizer in case the cable quenches [1]. About 10,000 such joints exist in the LHC. An extensive campaign has been undertaken to characterize and map the resistances of these joints. All of the superconducting cable splices were measured at 1.9 K and no splices were found with a resistance larger than 3 nW. Non-invasive measurements of the stabilizer joints were made at 300 K in 5 of the 8 sectors, and at 80 K in 3 sectors. More precise local measurements were made on suspect interconnects that were opened up, and poor joints were repaired. However, it is likely that additional imperfect stabilizer joints still exist in the LHC. A statistical analysis is used to place bounds on the remaining worst-case resistances. This sets limits on the maximum operating energy of the LHC, prior...

  2. Proposal for the award of a blanket contract for the supply and installation of water-cooled bus bars and cables for the LHC

    CERN Document Server

    2003-01-01

    This document concerns the award of a blanket contract for the supply and installation of water-cooled bus bars and cables for the LHC project. Following a market survey carried out among 22 firms in six Member States, a call for tenders (IT-2941/ST/LHC) was sent on 30 June 2003 to three firms in two Member States. By the closing date, CERN had received tenders from the three firms. The Finance Committee is invited to agree to the negotiation of a blanket contract with FLOHE (DE), the lowest bidder, for the supply and installation of water-cooled bus bars and cables, for a total amount not exceeding 2 900 000 Swiss francs, subject to revision after 1 January 2005 according to the LME copper prices. The firm has indicated the following distribution by country of the contract value covered by this adjudication proposal: DE - 100%.

  3. PEEC method dedicated to power bus-bar design; Conception des jeux de barres de puissance: interet de la methode PEEC en vue de l'optimisation

    Energy Technology Data Exchange (ETDEWEB)

    Gonnet, J.P.; Mazauric, V. [Schneider Electric Industries, 92 - Rueil-Malmaison (France); Guichon, J.M. [Ecole Nationale Superieure d' Ingenieurs Electriciens de Grenoble, Lab. d' Electrotechnique, 38 - Saint Martin d' Heres (France); Wendling, Ph. [CEDRAT SA, 38 - Meylan (France); MAGSOFT Corp., NY (United States)

    2006-04-15

    This paper presents the power of the PEEC (Partial Element Equivalent Circuit) method to deal with electrical distribution networks. Because the air regions are not meshed, this method is very efficient for modeling the electromagnetic behavior of bus-bar systems surrounded by large air volumes - i.e., any power distribution system. Furthermore, this method is shown to be efficient for both high and low frequency problems. Thus, the electromagnetic disturbances caused by wiring are evaluated. This method is not only a modeling method. It also enables the evaluation of the design of bus-bars or trunkings leading to the optimization of their layouts and even of their shapes for electromagnetic considerations. (authors)

  4. Stabilizer bar design about the rear suspension of a light bus%某轻型客车后悬架稳定杆设计

    Institute of Scientific and Technical Information of China (English)

    戴声良; 唐学东; 王长文; 李磊; 刘辉

    2009-01-01

    A stabilizer bar was designed based on one light bus single tilted arm rear suspension. Motion harmony and validity in suspension system are analyzed according to geometry relationship. The experiment results show that steering stability of the full vehicle is improved by the stabilizer bar, and the rolling angle stiffness between front and rear suspension matches more reasonably.%对某轻型客车单斜臂式后悬架布置横向稳定杆,根据几何关系分析了横向稳定杆在悬架系统中运动协调性和有效性;试验结果表明,设计的稳定杆能有效改善整车的操纵稳定性,前后悬架的侧倾角刚度匹配也更趋合理.

  5. 铜排连续挤压力能的工程法计算%Calculation of Pressure and Power Required by Continuous Extrusion of Copper Bus Bar by Slab Method

    Institute of Scientific and Technical Information of China (English)

    于孟; 代娜娜; 钟毅

    2012-01-01

    The pressure and power required by continuous extrusion of copper bus bar was calculated and the analytical expression of the inlet stress of the extending mould was obtained herein. The analytical expression shows the rules of the pressure changing with the variation of thickness and flakiness ratio of products. Taking the torque caused by flash of metal and plug into account, the power required by continuous extrusion of copper bus bar with a specification of 204. 5mm × 7. 5mm was calculated by employing the proposed method, and the results show good agreement with the experimental values. The error of calculation is 12%, and is able to meet the engineering application. Therefore, the proposed method can provide reference for the design of extrusion mould and rational selection of copper bus bar continuous extrusion machine.%采用工程法对连续挤压铜排的力能进行了计算,得到了连续挤压铜排模腔入口挤压应力的解析表达式及挤压应力随产品厚度和宽厚比变化的规律.考虑到溢料和堵头对挤压轮产生的扭矩,对204.5mm×7.5mm铜排连续挤压的功率进行了计算,并将其与实验中的实测值进行比较,二者误差为12%,能满足工程计算的需要.因此,该方法可为模具设计和合理选择铜排连续挤压设备提供参考.

  6. Splice Resistance Measurements in the LHC Main Superconducting Magnet Circuits by the New Quench Protection System

    CERN Document Server

    Charifoulline, Z; Denz, R; Siemko, A; Steckert, J

    2012-01-01

    The interconnections between the LHC main magnets are made of soldered joints (splices) of two superconducting cables stabilized by a copper bus-bar. After the 2008 LHC incident, caused by a defective interconnection, a new layer of high resolution magnet circuit quench protection (nQPS) has been developed and integrated with the existing systems. It allowed mapping of the resistances of all superconducting splices during the 2009 commissioning campaign. Since April 2010, when the LHC was successfully restarted at 3.5 TeV, every bus bar interconnection is constantly monitored by the nQPS electronics. The acquired data are saved to the LHC Logging Database. The paper will briefly describe the data analysis method and will present the results from the two years of resistance measurements. Although no splice was found with resistance higher than 3.3 n and no significant degradation in time was observed so far, the monitoring of splices will stay active till the end of LHC 4 TeV run. The detected outliers wil...

  7. A close-up of the lower part of a 13 kA current lead. The high-temperature superconductor (on the left in the photo) with the low-temperature superconductor (on the right). Resting in liquid helium, the low-temperature superconductor is connected to the bus-bars conveying the current to the LHC magnets.

    CERN Multimedia

    2004-01-01

    A close-up of the lower part of a 13 kA current lead. The high-temperature superconductor (on the left in the photo) with the low-temperature superconductor (on the right). Resting in liquid helium, the low-temperature superconductor is connected to the bus-bars conveying the current to the LHC magnets.

  8. ELECTRO-THERMAL AND MECHANICAL VALIDATION EXPERIMENT ON THE LHC MAIN BUSBAR SPLICE CONSOLIDATION

    CERN Document Server

    Willering, GP; Bourcey, N; Bottura, L; Charrondiere, M; Cerqueira Bastos, M; Deferne, G; Dib, G; Giloux, Chr; Grand-Clement, L; Heck, S; Hudson, G; Kudryavtsev, D; Perret, P; Pozzobon, M; Prin, H; Scheuerlein, Chr; Rijllart, A; Triquet, S; Verweij, AP

    2012-01-01

    To eliminate the risk of thermal runaways in LHC interconnections a consolidation by placing shunts on the main bus bar interconnections is proposed by the Task Force Splices Consolidation. To validate the design two special SSS magnet spares are placed on a test bench in SM-18 to measure the interconnection in between with conditions as close as possible to the LHC conditions. Two dipole interconnections are instrumented and prepared with worst-case-conditions to study the thermo-electric stability limits. Two quadrupole interconnections are instrumented and prepared for studying the effect of current cycling on the mechanical stability of the consolidation design. All 4 shunted interconnections showed very stable behaviour, well beyond the LHC design current cycle.

  9. PCI bus demystified

    CERN Document Server

    Abbott, Doug

    2004-01-01

    The peripheral component interconnect (PCI) bus is the dominant bus system used to connect the different elements making up today''s high-performance computer systems. Different PCI implementations have also been developed for such applications as telecommunications and embedded computing. If an application calls for high speed, high reliability, flexible configuration, and bus mastering, then PCI is the only logical bus choice. This book is an applications-oriented introduction to the PCI bus, with an emphasis on implementing PCI in a variety of computer architectures. Special attention is given to industrial and mission-critical applications of PCI bus.·Fully describes PCI electrical specifications, mechanical requirements, and signal types·Covers advanced topics through numerous design examples to increase the readers understanding of the subject ·Includes updated coverage of PCI-X 2.0

  10. Intelligent Bus Stops in the Flexible Bus Systems

    Directory of Open Access Journals (Sweden)

    Razi Iqbal

    2014-09-01

    Full Text Available The purpose of this paper is to discuss Intelligent Bus Stops in a special Demand Responsive Transit (DRT, the Flexible Bus System. These Intelligent Bus Stops are more efficient and information rich than Traditional Bus Stops. The real time synchronization of the Flexible Bus System makes it unique as compared to Traditional Bus Systems. The Main concern is to make Bus Stops intelligent and information rich. Buses are informed about the no. of passengers waiting at the upcoming Bus Stops. If there are no passengers to ride or get off on upcoming Bus Stop, the Bus can skip that Bus Stop and head towards the next Bus Stop where passenger is waiting, which will decrease the ride time of the passengers on the Bus and also the wait time of the passengers waiting on the upcoming Bus Stops. Providing more information at Bus Stops about the Destination (Time to Destination, Distance to Destination etc. and Buses (Bus Location, Arrival Time of Bus etc. makes it easier for the passengers to decide whether to ride a particular Bus or not.

  11. The Hidden Failure of BP-2B Numerical Bus-Bar Protection and Its Logic Optimization%BP-2B保护装置死区保护的隐患分析及其逻辑优化

    Institute of Scientific and Technical Information of China (English)

    黄有立; 崔志文

    2011-01-01

    实践发现在某些情况下发生死区故障时,BP-2B保护装置会出现误判,造成切除范围扩大。针对这一隐患进行分析,发现了该问题是由于其母联充电保护中的母联断路器预投逻辑导致,并时该逻辑的提出了改进方案,避免了非故障母线的误动和停电范围的扩大,具有一定的工程实用价值。%It happened in practice that when dead zone failures occurred in certain conditions, the BP-2B protection would make miscarriage of justice so to expand the outage dimension. Aiming at the hidden failure, this paper does analysis and finds out it is due to the logic of bus connection pre-closed, putting forward an improvement scheme for the logic so to avoid the maloperation of non failure bus and to limit the outage dimension, and thus to have certain engineering value.

  12. TPG bus passes

    CERN Multimedia

    Staff Association

    2013-01-01

    The CERN Staff Association will stop selling TPG bus passes. All active and retired members of the CERN personnel will be able to purchase Unireso bus passes from the CERN Hostel - Building 39 (Meyrin site) from 1st February 2013. For more information: https://cds.cern.ch/journal/CERNBulletin/2013/04/Announcements/1505279?ln=en

  13. Savannah River bus project

    Energy Technology Data Exchange (ETDEWEB)

    Summers, W.A. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1998-08-01

    The H2Fuel Bus is the world`s first hybrid hydrogen electric transit bus. It was developed through a public/private partnership involving several leading technology and industrial organizations in the Southeast, with primary funding and program management provided by the Department of Energy. The primary goals of the project are to gain valuable information on the technical readiness and economic viability of hydrogen buses and to enhance the public awareness and acceptance of emerging hydrogen technologies. The bus has been operated by the transit agency in Augusta, Georgia since April, 1997. It employs a hybrid IC engine/battery/electric drive system, with onboard hydrogen fuel storage based on the use of metal hydrides. Initial operating results have demonstrated an overall energy efficiency (miles per Btu) of twice that of a similar diesel-fueled bus and an operating range twice that of an all-battery powered electric bus. Tailpipe emissions are negligible, with NOx less than 0.2 ppm. Permitting, liability and insurance issues were addressed on the basis of extensive risk assessment and safety analyses, with the inherent safety characteristic of metal hydride storage playing a major role in minimizing these concerns. Future plans for the bus include continued transit operation and use as a national testbed, with potential modifications to demonstrate other hydrogen technologies, including fuel cells.

  14. LHC on the bus

    CERN Multimedia

    Laëtitia Pedroso

    2010-01-01

    On 15 December, an airport bus will be transformed in the image of CERN. The bus will be seen by the thousands of travellers arriving in Geneva, informing them of the possibility to visit CERN.   Sketch of the bus. The good relationship between Geneva International Airport and CERN started several years ago. In 2004 the airport put advertising space in the arrivals area at CERN's disposal free of charge. Now, starting on 15 December, a 40-foot long bus will display a giant sticker advertisement depicting CERN as it takes passengers over the airport tarmac to their planes. This is no ordinary sticker, and it was no mean task to attach it to the bus. The task of producing and attaching it was entrusted to Geneva-based specialists Mathys SA. With the ski season opening on 15 December, there will be many travellers arriving at the airport, and the bus will be ready to receive them. When one thinks of CERN, the subjects that naturally come to mind are the LHC, the mysteries of the Universe...

  15. BUS 100 Introduction To Business Entire Course

    OpenAIRE

    Laynebaril

    2017-01-01

      BUS 100 Introduction To Business Entire Course Click Link Below To Buy: http://hwcampus.com/shop/bus-100-introduction-to-business-entire-course/   BUS 100 Assignment 1 Fundamentals of Effective Communication in the Workplace BUS 100 Assignment 2 Ethical and Socially Responsive Business BUS 100 Introduction To Business Week 1-11 Discussions BUS 100 week 1 discussions BUS 100 week 2 Discussions BUS 100 week 3 Discussions BUS 100 week 4 Discussions ...

  16. Modeling the Coordinated Operation between Bus Rapid Transit and Bus

    OpenAIRE

    Jiaqing Wu; Rui Song; Youan Wang; Feng Chen; Shubin Li

    2015-01-01

    The coordination between bus rapid transit (BRT) and feeder bus service is helpful in improving the operational efficiency and service level of urban public transport system. Therefore, a coordinated operation model of BRT and bus is intended to develop in this paper. The total costs are formulated and optimized by genetic algorithm. Moreover, the skip-stop BRT operation is considered when building the coordinated operation model. A case of the existing bus network in Beijing is studied, the ...

  17. BUS 438 Concepts of Emerging Markets

    OpenAIRE

    Laynebaril

    2017-01-01

    BUS 438 Concepts of Emerging Markets Click Link Below To Buy: http://hwcampus.com/shop/bus-438-concepts-of-emerging-markets/   BUS438 BUS/438  MODULE 3 ASSIGNMENT 3 Concepts of Emerging Markets [ARGOSY

  18. Personal Computer Monitors Instrumentation Bus

    Science.gov (United States)

    Conroy, Bruce L.

    1994-01-01

    IBM-compatible personal computer used instead of logic analyzer or other special instrument to monitor IEEE-488 interface data bus that interconnects various pieces of laboratory equipment. Needed is short program for computer, commercial general-purpose interface bus circuit card, and adapter cable to link card to bus. Software available in Ada or Quick Basic language.

  19. Learning NServiceBus

    CERN Document Server

    Boike, David

    2013-01-01

    This is a practical tutorial containing hands-on examples for creating a messaging and SOA based service bus.This book is for .NET developers who are looking for ways to overcome problems related to buggy third party web service integrations, codebases that have grown into a big ball of mud, and batch jobs failure.

  20. Behavior Of A Confined Tension Lap Splice In High-Strength Reinforced Concrete Beams

    Science.gov (United States)

    Abdel-Kareem, Ahmed H.; Abousafa, Hala; El-Hadidi, Omaia S.

    2015-09-01

    The results of an experimental program conducted on seventeen simply supported concrete beams to study the effect of transverse reinforcement on the behavior of the lap splice of a steel reinforcement in tension zones in high-strength concrete beams are presented. The parameters included in the experimental program were the concrete compressive strength, the lap splice length, the amount of transverse reinforcement provided within the splice region, and the shape of the transverse reinforcement around the spliced bars. The experimental results showed that the displacement ductility increased and the mode of failure changed from a splitting bond failure to a flexural failure when the amount of the transverse reinforcement in the splice region increased, and the compressive strength increased up to 100 MPa. The presence of the transverse reinforcement around the spliced bars had a pronounced effect on increasing the ultimate load, the ultimate deflection, and the displacement ductility. The prediction of maximum steel stresses for spliced bars using the ACI 318-05 building code was compared with the experimental results. The comparison showed that the effect of the transverse reinforcement around spliced bars has to be considered into the design equations for lap splice length in high-strength concrete beams.

  1. BEIJING BARS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Cappuccino Bar Cappuccino Bar, at the intersection of Dongzhimenwai Dajie and Sanlitun Beijie, Beijing's most vibrant nightlife area, is a hot new destination for European food lovers. With its perfect combination of both energy and tranquility, and surrounded by diplomatic compounds, it is conveniently located near other Beijing destinations including Lady Street

  2. Modeling the Coordinated Operation between Bus Rapid Transit and Bus

    Directory of Open Access Journals (Sweden)

    Jiaqing Wu

    2015-01-01

    Full Text Available The coordination between bus rapid transit (BRT and feeder bus service is helpful in improving the operational efficiency and service level of urban public transport system. Therefore, a coordinated operation model of BRT and bus is intended to develop in this paper. The total costs are formulated and optimized by genetic algorithm. Moreover, the skip-stop BRT operation is considered when building the coordinated operation model. A case of the existing bus network in Beijing is studied, the proposed coordinated operation model of BRT and bus is applied, and the optimized headway and costs are obtained. The results show that the coordinated operation model could effectively decrease the total costs of the transit system and the transfer time of passengers. The results also suggest that the coordination between the skip-stop BRT and bus during peak hour is more effective than non-coordination operation.

  3. BBIS: Beacon Bus Information System

    Science.gov (United States)

    Kasim, Shahreen; Hafit, Hanayanti; Pei Juin, Kong; Afizah Afif, Zehan; Hashim, Rathiah; Ruslai, Husni; Jahidin, Kamaruzzaman; Syafwan Arshad, Mohammad

    2016-11-01

    Lack of bus information for example bus timetable, status of the bus and messy advertisement on bulletin board at the bus stop will give negative impact to tourist. Therefore, a real-time update bus information bulletin board provides all information needed so that passengers can save their bus information searching time. Supported with Android or iOS, Beacon Bus Information System (BBIS) provides bus information between Batu Pahat and Kluang area. BBIS is a system that implements physical web technology and interaction on demand. It built on Backend-as-a-Service, a cloud solution and Firebase non relational database as data persistence backend and syncs between user client in the real-time. People walk through bus stop with smart device and do not require any application. Bluetooth Beacon is used to achieve smart device's best performance of data sharing. Intellij IDEA 15 is one of the tools that that used to develop the BBIS system. Multi-language included front end and backend supported Integration development environment (IDE) helped to speed up integration process.

  4. 77 FR 48592 - Bus and Bus Facilities Discretionary Program Funds

    Science.gov (United States)

    2012-08-14

    ... authorized by 49 U.S.C. 5309(b), as amended by Section 3011 of the Safe, Affordable, Flexible, Efficient... Livability Initiatives (77 FR 6178). The NOFA explained the requirements and procedures for eligible... facilities, bus-related equipment, and transit asset management systems. The FY 2012 Bus...

  5. PERFORMANCE OF BUS LANES IN SEOUL

    Directory of Open Access Journals (Sweden)

    Hyung Jin KIM

    2003-01-01

    In this paper, the performance impacts of reserved bus lanes in Seoul were evaluated and several suggestions for improving the effectiveness of the bus preferential treatment are proposed. The impacts of reserved bus lanes on both bus and auto performance were primarily measured by the relative speed changes of buses and autos and the average circulation rate of bus trips. Empirical results showed that the bus lanes were successful in improving average bus performance compared with other adjacent traffic. It was confirmed that right turning movement of the buses would cause the deterioration of bus speed in the curbside bus lanes. The average circulation rate of daily bus trips during the bus operation periods was somewhat increased by the bus lane introduction. A current warrant for reserved bus lanes in Korea was reviewed, and average bus speed during peak periods was proposed as a new warrant. In order to improve the effectiveness of the reserved bus lane, the type of lane markings in reserved bus lane, enforcement strategies for illegal parking in the bus lane and FIFO service at bus stops are discussed.

  6. Learning NServiceBus Sagas

    CERN Document Server

    Helton, Rich

    2015-01-01

    If you are an Enterprise C# developer who wishes to extend your knowledge of NServiceBus and Enterprise Service Bus in C#, this is the book for you. This book is designed to enhance the education of ESBs and their messaging, whether you are a beginner or a seasoned expert in Enterprise C#, Apex, and Visualforce pages.

  7. Introduction to cotranscriptional RNA splicing.

    Science.gov (United States)

    Merkhofer, Evan C; Hu, Peter; Johnson, Tracy L

    2014-01-01

    The discovery that many intron-containing genes can be cotranscriptionally spliced has led to an increased understanding of how splicing and transcription are intricately intertwined. Cotranscriptional splicing has been demonstrated in a number of different organisms and has been shown to play roles in coordinating both constitutive and alternative splicing. The nature of cotranscriptional splicing suggests that changes in transcription can dramatically affect splicing, and new evidence suggests that splicing can, in turn, influence transcription. In this chapter, we discuss the mechanisms and consequences of cotranscriptional splicing and introduce some of the tools used to measure this process.

  8. spliceR

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Porse, Bo Torben; Sandelin, Albin;

    2014-01-01

    RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternative splicing...... and prediction of coding potential....

  9. The Myth of the "Ideal Bus"

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Does as an ideal bus technology exist? Many companies have and will promote a single technology as the ideal bus that will meet all application needs, but in actuality, each bus technology has its own strengths and weaknesses and thus is appropriate for different applications. History has shown the folly of claiming an ideal bus for every application.

  10. Performance of Grouted Splice Sleeve Connector under Tensile Load

    Directory of Open Access Journals (Sweden)

    A. Alias

    2016-05-01

    Full Text Available The grouted splice sleeve connector system takes advantage of the bond-slip resistance of the grout and the mechanical gripping of reinforcement bars to provide resistance to tensile force. In this system, grout acts as a load-transferring medium and bonding material between the bars and sleeve. This study adopted the end-to-end rebars connection method to investigate the effect of development length and sleeve diameter on the bonding performance of the sleeve connector. The end-to-end method refers to the condition where reinforcement bars are inserted into the sleeve from both ends and meet at the centre before grout is filled. Eight specimens of grouted splice sleeve connector were tested under tensile load to determine their performance. The sleeve connector was designed using 5 mm thick circular hollow section (CHS steel pipe and consisted of one external and two internal sleeves. The tensile test results show that connectors with a smaller external and internal sleeve diameter appear to provide better bonding performance. Three types of failure were observed in this research, which are bar fracture (outside the sleeve, bar pullout, and internal sleeve pullout. With reference to these failure types, the development length of 200 mm is the optimum value due to its bar fracture type, which indicates that the tensile capacity of the connector is higher than the reinforcement bar. It is found that the performance of the grouted splice sleeve connector is influenced by the development length of the reinforcement bar and the diameter of the sleeve.

  11. High-Speed Ring Bus

    Science.gov (United States)

    Wysocky, Terry; Kopf, Edward, Jr.; Katanyoutananti, Sunant; Steiner, Carl; Balian, Harry

    2010-01-01

    The high-speed ring bus at the Jet Propulsion Laboratory (JPL) allows for future growth trends in spacecraft seen with future scientific missions. This innovation constitutes an enhancement of the 1393 bus as documented in the Institute of Electrical and Electronics Engineers (IEEE) 1393-1999 standard for a spaceborne fiber-optic data bus. It allows for high-bandwidth and time synchronization of all nodes on the ring. The JPL ring bus allows for interconnection of active units with autonomous operation and increased fault handling at high bandwidths. It minimizes the flight software interface with an intelligent physical layer design that has few states to manage as well as simplified testability. The design will soon be documented in the AS-1393 standard (Serial Hi-Rel Ring Network for Aerospace Applications). The framework is designed for "Class A" spacecraft operation and provides redundant data paths. It is based on "fault containment regions" and "redundant functional regions (RFR)" and has a method for allocating cables that completely supports the redundancy in spacecraft design, allowing for a complete RFR to fail. This design reduces the mass of the bus by incorporating both the Control Unit and the Data Unit in the same hardware. The standard uses ATM (asynchronous transfer mode) packets, standardized by ITU-T, ANSI, ETSI, and the ATM Forum. The IEEE-1393 standard uses the UNI form of the packet and provides no protection for the data portion of the cell. The JPL design adds optional formatting to this data portion. This design extends fault protection beyond that of the interconnect. This includes adding protection to the data portion that is contained within the Bus Interface Units (BIUs) and by adding to the signal interface between the Data Host and the JPL 1393 Ring Bus. Data transfer on the ring bus does not involve a master or initiator. Following bus protocol, any BIU may transmit data on the ring whenever it has data received from its host. There

  12. Scaling Laws in Chennai Bus Network

    CERN Document Server

    Chatterjee, Atanu

    2015-01-01

    In this paper, we study the structural properties of the complex bus network of Chennai. We formulate this extensive network structure by identifying each bus stop as a node, and a bus which stops at any two adjacent bus stops as an edge connecting the nodes. Rigorous statistical analysis of this data shows that the Chennai bus network displays small-world properties and a scale-free degree distribution with the power-law exponent, $\\gamma > 3$.

  13. SCHOOL BUS GARAGES, 1966 REVISION.

    Science.gov (United States)

    JOHNSON, HERBERT F.

    STANDARDS AND RECOMMENDATIONS ARE GIVEN FOR THE DESIGN AND CONSTRUCTION OF SCHOOL BUS GARAGES INCLUDING BRIEF DISCUSSIONS OF--(1) SITE DEVELOPMENT, (2) DESIGN AND CONSTRUCTION, AND (3) MECHANICAL (HEATING, PLUMBING, AND VENTILATION) AND ELECTRICAL FACTORS. (JT)

  14. Optical Fiber Fusion Splicing

    CERN Document Server

    Yablon, Andrew D

    2005-01-01

    This book is an up-to-date treatment of optical fiber fusion splicing incorporating all the recent innovations in the field. It provides a toolbox of general strategies and specific techniques that the reader can apply when optimizing fusion splices between novel fibers. It specifically addresses considerations important for fusion splicing of contemporary specialty fibers including dispersion compensating fiber, erbium-doped gain fiber, polarization maintaining fiber, and microstructured fiber. Finally, it discusses the future of optical fiber fusion splicing including silica and non-silica based optical fibers as well as the trend toward increasing automation. Whilst serving as a self-contained reference work, abundant citations from the technical literature will enable readers to readily locate primary sources.

  15. Wishbone bus Architecture - A Survey and Comparison

    CERN Document Server

    Sharma, Mohandeep; 10.5121/vlsic.2012.3210

    2012-01-01

    The performance of an on-chip interconnection architecture used for communication between IP cores depends on the efficiency of its bus architecture. Any bus architecture having advantages of faster bus clock speed, extra data transfer cycle, improved bus width and throughput is highly desirable for a low cost, reduced time-to-market and efficient System-on-Chip (SoC). This paper presents a survey of WISHBONE bus architecture and its comparison with three other on-chip bus architectures viz. Advanced Micro controller Bus Architecture (AMBA) by ARM, CoreConnect by IBM and Avalon by Altera. The WISHBONE Bus Architecture by Silicore Corporation appears to be gaining an upper edge over the other three bus architecture types because of its special performance parameters like the use of flexible arbitration scheme and additional data transfer cycle (Read-Modify-Write cycle). Moreover, its IP Cores are available free for use requiring neither any registration nor any agreement or license.

  16. Alternative splicing interference by xenobiotics.

    Science.gov (United States)

    Zaharieva, Emanuela; Chipman, J Kevin; Soller, Matthias

    2012-06-14

    The protein coding sequence of most eukaryotic genes (exons) is interrupted by non-coding parts (introns), which are excised in a process termed splicing. To generate a mature messenger RNA (mRNA) hundreds of combinatorial protein-protein and RNA-protein interactions are required to splice out often very large introns with high fidelity and accuracy. Inherent to splicing is the use of alternative splice sites generating immense proteomic diversity from a limited number of genes. In humans, alternative splicing is a major mode of regulating gene expression, occurs in over 90% of genes and is particularly abundant in the brain. Only recently, it has been recognized that the complexity of the splicing process makes it susceptible to interference by various xenobiotics. These compounds include antineoplastic substances, commonly used drugs and food supplements and cause a spectrum of effects ranging from deleterious inhibition of general splicing to highly specific modifications of alternative splicing affecting only certain genes. Alterations in splicing have been implicated in numerous diseases such as cancer and neurodegeneration. Splicing regulation plays an important role in the execution of programmed cell death. The switch between anti- and pro-apoptotic isoforms by alternative splice site selection and misregulation of a number of splicing factors impacts on cell survival and disease. Here, our current knowledge is summarized on compounds interfering with general and alternative splicing and of the current methodology to study changes in these processes relevant to the field of toxicology and future risk assessments.

  17. Aids to School Bus Design

    Science.gov (United States)

    1991-01-01

    Navistar International Transportation Corporation, Chicago, IL, used three separate NASA-developed technologies in the design and testing of their 3000 Series Bus Chassis which was developed expressly for school bus applications. For structural analysis, they used the MSC/NASTRAN program which mathematically analyzes a design and predicts how it will hold up under stress. They also used the SPATE 9000 system for non-contact measurement of stress, load transfer mechanisms, detection of hidden flaws, and monitoring structural changes during fatigue testing. SPATE 9000 was based on infrared stress measurement technology developed by Langley Research Center. They also employed the Wyle Ride Quality Meter, which was developed by Langley to aid in passenger aircraft design by providing an accurate measurement of ride vibration and sound level. These numbers translate into a subjective discomfort level index. These technologies contribute to the company's 45-48 percent share of the school bus chassis market.

  18. School bus and children's traffic safety

    Institute of Scientific and Technical Information of China (English)

    PAN Shu-ming; Stephen Hargarten; ZHU Shan-kuan

    2007-01-01

    There is no safer way to transport a child than a school bus. Fatal crashes involving occupants are extremely rare events in the US. In recent years, school bus transportation began to develop in China. We want to bring advanced experience on school bus safety in Western countries such as the US to developing countries.Methods: We searched the papers related to school bus safety from Medline, Chinese Scientific Journals Database and the Web of the National Highway Traffic Safety Administration (NHTSA).Results: There were only 9 papers related to school bus safety, which showed that higher levels of safety standards on school buses, school bus-related transportation and environmental laws and injury prevention were the primary reasons for the desired outcome. Few school bus is related to deaths and injuries in the developed countries.Conclusions: The developing countries should make strict environmental laws and standards on school bus safety to prevent children's injury and death.

  19. SpliceDisease database: linking RNA splicing and disease.

    Science.gov (United States)

    Wang, Juan; Zhang, Jie; Li, Kaibo; Zhao, Wei; Cui, Qinghua

    2012-01-01

    RNA splicing is an important aspect of gene regulation in many organisms. Splicing of RNA is regulated by complicated mechanisms involving numerous RNA-binding proteins and the intricate network of interactions among them. Mutations in cis-acting splicing elements or its regulatory proteins have been shown to be involved in human diseases. Defects in pre-mRNA splicing process have emerged as a common disease-causing mechanism. Therefore, a database integrating RNA splicing and disease associations would be helpful for understanding not only the RNA splicing but also its contribution to disease. In SpliceDisease database, we manually curated 2337 splicing mutation disease entries involving 303 genes and 370 diseases, which have been supported experimentally in 898 publications. The SpliceDisease database provides information including the change of the nucleotide in the sequence, the location of the mutation on the gene, the reference Pubmed ID and detailed description for the relationship among gene mutations, splicing defects and diseases. We standardized the names of the diseases and genes and provided links for these genes to NCBI and UCSC genome browser for further annotation and genomic sequences. For the location of the mutation, we give direct links of the entry to the respective position/region in the genome browser. The users can freely browse, search and download the data in SpliceDisease at http://cmbi.bjmu.edu.cn/sdisease.

  20. spliceR

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Porse, Bo Torben; Sandelin, Albin Gustav;

    2014-01-01

    BACKGROUND: RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternat...

  1. Low-inductance bus lines

    Science.gov (United States)

    Kernick, A.

    1977-01-01

    Laminated bus strips and bifilar litz cable connectors for high-power rectifiers, thrisistors, and transistors provide low inductance and eliminate electromagnetic interference in high-power circuits. These techniques offer significant cost advantages because of ease of assembly and consistent high quality of product. Effectiveness makes general usage in static power conversion likely.

  2. HERMES travels by CAN bus

    Science.gov (United States)

    Waller, Lewis G.; Shortridge, Keith; Farrell, Tony J.; Vuong, Minh; Muller, Rolf; Sheinis, Andrew I.

    2014-07-01

    The new HERMES spectrograph represents the first foray by AAO into the use of commercial off-the-shelf industrial field bus technology for instrument control, and we regard the final system, with its relatively simple wiring requirements, as a great success. However, both software and hardware teams had to work together to solve a number of problems integrating the chosen CANopen/CAN bus system into our normal observing systems. A Linux system running in an industrial PC chassis ran the HERMES control software, using a PCI CAN bus interface connected to a number of distributed CANopen/CAN bus I/O devices and servo amplifiers. In the main, the servo amplifiers performed impressively, although some experimentation with homing algorithms was required, and we hit a significant hurdle when we discovered that we needed to disable some of the encoders used during observations; we learned a lot about how servo amplifiers respond when their encoders are turned off, and about how encoders react to losing power. The software was based around a commercial CANopen library from Copley Controls. Early worries about how this heavily multithreaded library would work with our standard data acquisition system led to the development of a very low-level CANopen software simulator to verify the design. This also enabled the software group to develop and test almost all the control software well in advance of the construction of the hardware. In the end, the instrument went from initial installation at the telescope to successful commissioning remarkably smoothly.

  3. Analysis of robustness of urban bus network

    Science.gov (United States)

    Tao, Ren; Yi-Fan, Wang; Miao-Miao, Liu; Yan-Jie, Xu

    2016-02-01

    In this paper, the invulnerability and cascade failures are discussed for the urban bus network. Firstly, three static models(bus stop network, bus transfer network, and bus line network) are used to analyse the structure and invulnerability of urban bus network in order to understand the features of bus network comprehensively. Secondly, a new way is proposed to study the invulnerability of urban bus network by modelling two layered networks, i.e., the bus stop-line network and the bus line-transfer network and then the interactions between different models are analysed. Finally, by modelling a new layered network which can reflect the dynamic passenger flows, the cascade failures are discussed. Then a new load redistribution method is proposed to study the robustness of dynamic traffic. In this paper, the bus network of Shenyang City which is one of the biggest cities in China, is taken as a simulation example. In addition, some suggestions are given to improve the urban bus network and provide emergency strategies when traffic congestion occurs according to the numerical simulation results. Project supported by the National Natural Science Foundation of China (Grant Nos. 61473073, 61374178, 61104074, and 61203329), the Fundamental Research Funds for the Central Universities (Grant Nos. N130417006, L1517004), and the Program for Liaoning Excellent Talents in University (Grant No. LJQ2014028).

  4. Electrical system architecture having high voltage bus

    Science.gov (United States)

    Hoff, Brian Douglas; Akasam, Sivaprasad

    2011-03-22

    An electrical system architecture is disclosed. The architecture has a power source configured to generate a first power, and a first bus configured to receive the first power from the power source. The architecture also has a converter configured to receive the first power from the first bus and convert the first power to a second power, wherein a voltage of the second power is greater than a voltage of the first power, and a second bus configured to receive the second power from the converter. The architecture further has a power storage device configured to receive the second power from the second bus and deliver the second power to the second bus, a propulsion motor configured to receive the second power from the second bus, and an accessory motor configured to receive the second power from the second bus.

  5. PinBus Interface Design

    Energy Technology Data Exchange (ETDEWEB)

    Hammerstrom, Donald J.; Adgerson, Jewel D.; Sastry, Chellury; Pratt, Richard M.; Pratt, Robert G.

    2009-12-30

    On behalf of the U.S. Department of Energy, PNNL has explored and expanded upon a simple control interface that might have merit for the inexpensive communication of smart grid operational objectives (demand response, for example) to small electric end-use devices and appliances. The approach relies on bi-directional communication via the electrical voltage states of from one to eight shared interconnection pins. The name PinBus has been suggested and adopted for the proposed interface protocol. The protocol is defined through the presentation of state diagrams and the pins’ functional definitions. Both simulations and laboratory demonstrations are being conducted to demonstrate the elegance and power of the suggested approach. PinBus supports a very high degree of interoperability across its interfaces, allowing innumerable pairings of devices and communication protocols and supporting the practice of practically any smart grid use case.

  6. New TPG bus route 28

    CERN Multimedia

    2003-01-01

    Geneva's Public Transport services (TPG) have recently inaugurated a new bus line 28, connecting the La Tour Hospital in Meyrin to the international organisations in Geneva, via the airport. All signs associated with this route will be yellow in colour. Timetables and route details can be found at http://www.tpg.ch. Relations with the Host States Service http://www.cern.ch/relations/ Tel. 72848

  7. Avionic Data Bus Integration Technology

    Science.gov (United States)

    1991-12-01

    address the hardware-software interaction between a digital data bus and an avionic system. Very Large Scale Integration (VLSI) ICs and multiversion ...the SCP. In 1984, the Sperry Corporation developed a fault tolerant system which employed multiversion programming, voting, and monitoring for error... MULTIVERSION PROGRAMMING. N-version programming. 226 N-VERSION PROGRAMMING. The independent coding of a number, N, of redundant computer programs that

  8. Bus Routes, Marta Bus Routes located in Transportation database, Published in unknown, City of Roswell, GA.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset as of unknown. It is described as 'Marta Bus Routes located in Transportation database'. Data by this publisher are often provided in State...

  9. YUTONG BUS: Grateful To The Financial Crisis

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Shenyin&Wanguo Securities issued an announcement on Jan 9 rating Yutong Bus(600066)as"buying",on the basis that the bus maker's gross profits went better in the last quarter of 2008 and to be further improved in the first quarter of 2009,with an increased market share. The report drew attention inside the in- dustry when the entire bus segment was suffering a tough time.

  10. SAGE2Splice: unmapped SAGE tags reveal novel splice junctions.

    Directory of Open Access Journals (Sweden)

    Byron Yu-Lin Kuo

    2006-04-01

    Full Text Available Serial analysis of gene expression (SAGE not only is a method for profiling the global expression of genes, but also offers the opportunity for the discovery of novel transcripts. SAGE tags are mapped to known transcripts to determine the gene of origin. Tags that map neither to a known transcript nor to the genome were hypothesized to span a splice junction, for which the exon combination or exon(s are unknown. To test this hypothesis, we have developed an algorithm, SAGE2Splice, to efficiently map SAGE tags to potential splice junctions in a genome. The algorithm consists of three search levels. A scoring scheme was designed based on position weight matrices to assess the quality of candidates. Using optimized parameters for SAGE2Splice analysis and two sets of SAGE data, candidate junctions were discovered for 5%-6% of unmapped tags. Candidates were classified into three categories, reflecting the previous annotations of the putative splice junctions. Analysis of predicted tags extracted from EST sequences demonstrated that candidate junctions having the splice junction located closer to the center of the tags are more reliable. Nine of these 12 candidates were validated by RT-PCR and sequencing, and among these, four revealed previously uncharacterized exons. Thus, SAGE2Splice provides a new functionality for the identification of novel transcripts and exons. SAGE2Splice is available online at http://www.cisreg.ca.

  11. Methods for Characterization of Alternative RNA Splicing.

    Science.gov (United States)

    Harvey, Samuel E; Cheng, Chonghui

    2016-01-01

    Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing "minigene" in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest.

  12. Mutual interdependence of splicing and transcription elongation.

    Science.gov (United States)

    Brzyżek, Grzegorz; Świeżewski, Szymon

    2015-01-01

    Transcription and splicing are intrinsically linked, as splicing needs a pre-mRNA substrate to commence. The more nuanced view is that the rate of transcription contributes to splicing regulation. On the other hand there is accumulating evidence that splicing has an active role in controlling transcription elongation by DNA-dependent RNA polymerase II (RNAP II). We briefly review those mechanisms and propose a unifying model where splicing controls transcription elongation to provide an optimal timing for successive rounds of splicing.

  13. Wishbone Bus Architecture - A Survey and Comparison

    Directory of Open Access Journals (Sweden)

    Mohandeep Sharma

    2012-05-01

    Full Text Available The performance of an on-chip interconnection architecture used for communication between IP cores depends on the efficiency of its bus architecture. Any bus architecture having advantages of faster bus clock speed, extra data transfer cycle, improved bus width and throughput is highly desirable for a low cost, reduced time-to-market and efficient System-on-Chip (SoC. This paper presents a survey of WISHBONE bus architecture and its comparison with three other on-chip bus architectures viz. Advanced Microcontroller Bus Architecture (AMBA by ARM, CoreConnect by IBM and Avalon by Altera. The WISHBONE Bus Architecture by Silicore Corporation appears to be gaining an upper edge over the other three bus architecture types because of its special performance parameters like the use of flexible arbitration scheme and additional data transfer cycle (Read-Modify-Write cycle. Moreover, its IP Cores are available free for use requiring neither any registration nor any agreement or license.

  14. Barred Owl [ds8

    Data.gov (United States)

    California Department of Resources — These data define the current range of Barred and hybrid Barred/Spotted Owls in California. The current range includes the coastal mountains of northern California...

  15. A new bus lane on urban expressway with no-bay bus stop

    Science.gov (United States)

    Tian, Zhao; Jia, Limin

    2016-01-01

    The sharp increase in residents and vehicles causes heavy traffic pressure in many cities. To ease traffic congestion, it has been the common sense that we should develop public transit system. The priority of the bus appears particularly necessary with the rapid development of the public transport system. The bus lane is an important embodiment of the bus priority. Focusing on the problem of the unreasonable dedicated bus lane (DBL) under the lower ratio of buses, this paper proposed a new bus lane with limited physical length. And this bus lane can reduce the lane-changing conflict caused by the buses and cars running on roads without bus lanes. Based on the cellular automata (CA) traffic flow model and the lane-changing behavior of the vehicle including the optional lane-changing and the mandatory lane-changing, a three-lane traffic model with an isolated no-bay bus stop is proposed. The ordinary three-lane traffic without a bus lane and the cases of traffic with a DBL or the proposed bus lane are simulated, and the comparisons in the form of the fundamental diagrams are made among them. It is shown that the no-bay bus stop can act as a bottleneck on the traffic flow because of the mandatory lane-changing behavior. Under a certain ratio of the bus number to the total vehicles number, (1) the traffic with the proposed bus lane has less lane-changing conflict and can provide higher traffic capacity than the ordinary traffic without a bus lane, (2) compared with the DBL, the proposed bus lane is advantageous in easing congestion on the ordinary lanes when the traffic flow is high and can avoid unreasonable allocation of the road resources.

  16. Road safety issues for bus transport management.

    Science.gov (United States)

    Cafiso, Salvatore; Di Graziano, Alessandro; Pappalardo, Giuseppina

    2013-11-01

    Because of the low percentage of crashes involving buses and the assumption that public transport improves road safety by reducing vehicular traffic, public interest in bus safety is not as great as that in the safety of other types of vehicles. It is possible that less attention is paid to the significance of crashes involving buses because the safety level of bus systems is considered to be adequate. The purpose of this study was to evaluate the knowledge and perceptions of bus managers with respect to safety issues and the potential effectiveness of various technologies in achieving higher safety standards. Bus managers were asked to give their opinions on safety issues related to drivers (training, skills, performance evaluation and behaviour), vehicles (maintenance and advanced devices) and roads (road and traffic safety issues) in response to a research survey. Kendall's algorithm was used to evaluate the level of concordance. The results showed that the majority of the proposed items were considered to have great potential for improving bus safety. The data indicated that in the experience of the participants, passenger unloading and pedestrians crossing near bus stops are the most dangerous actions with respect to vulnerable users. The final results of the investigation showed that start inhibition, automatic door opening, and the materials and internal architecture of buses were considered the items most strongly related to bus passenger safety. Brake assistance and vehicle monitoring systems were also considered to be very effective. With the exception of driver assistance systems for passenger and pedestrian safety, the perceptions of the importance of other driver assistance systems for vehicle monitoring and bus safety were not unanimous among the bus company managers who participated in this survey. The study results showed that the introduction of new technologies is perceived as an important factor in improving bus safety, but a better understanding

  17. On Modified Bar recursion

    DEFF Research Database (Denmark)

    Oliva, Paulo Borges

    2002-01-01

    Modified bar recursion is a variant of Spector's bar recursion which can be used to give a realizability interpretation of the classical axiom of dependent choice. This realizability allows for the extraction of witnesses from proofs of forall-exists-formulas in classical analysis. In this talk I...... shall report on results regarding the relationship between modified and Spector's bar recursion. I shall also show that a seemingly weak form of modified bar recursion is as strong as "full" modified bar recursion in higher types....

  18. Design of intelligent bus stop based on GPRS

    Institute of Scientific and Technical Information of China (English)

    张思远

    2013-01-01

    To provide people waiting on the bus stop the information about the number of passengers on the bus traffic and distance between the site and the running bus. Design a set of intelligent bus stop system. This system counts up the number of the people gets on the bus and the people getting out of the bus to add up the people on the bus by infrared sensor and uses the hall sensor to measure the distance of having driven. Then, send data via GPRS. Finally display on the corresponding bus stop.

  19. Mastering NServiceBus and persistence

    CERN Document Server

    Helton, Rich

    2014-01-01

    This book is intended for developers, designers, and architects alike who wish to build C# NServiceBus enterprise architectures and learn how ESB persists data and messages to help them attain their goals. No prior knowledge of persistence in NServiceBus is required.

  20. From the hybrid bus to the fully electrical bus; Vom Hybridbus zum vollelektrischen Bus

    Energy Technology Data Exchange (ETDEWEB)

    Soffel, Christian [VCDB VerkehrsConsult Dresden-Berlin GmbH, Dresden (Germany). Team Elektromobilitaet

    2013-05-15

    To an increasing extent, hybrid buses have become omnipresent in conurbations as far as public transport is concerned. However, they are only an intermediate stage on the path to the fully electrical bus. The development path has been marked out and depends on the progress made in energy storage technology and automobile industry. From an economic point of view, practicable and adequate funding measures are required for the time being for precisely arranging electromobility in public transport and thus before an audience of millions. (orig.)

  1. Observation of B0bar --> D0 K0bar and B0bar --> D0 K*0bar decays

    CERN Document Server

    Krokovnyi, P P

    2003-01-01

    We report on a search for B0bar --> D0 K(*)0bar decays based on 85 10^6 BBar events collected with the Belle detector at KEKB. The B0bar --> D0 K0bar and B0bar --> D0 K*0bar decays have been observed for the first time with the branching fractions Br(B0bar --> D0 K0bar) = (5.0^{+1.3}_{-1.2}+- 0.6) 10^{-5} and Br(B0bar --> D0 K*0bar) = (4.8^{+1.1}_{-1.0}+- 0.5) 10^{-5}. No significant signal has been found for the B0bar --> D*0 K(*)0bar and B0bar --> D(*)0bar K*0bar decay modes, and upper limits at 90% CL are presented.

  2. Intronic Alus influence alternative splicing.

    Directory of Open Access Journals (Sweden)

    Galit Lev-Maor

    Full Text Available Examination of the human transcriptome reveals higher levels of RNA editing than in any other organism tested to date. This is indicative of extensive double-stranded RNA (dsRNA formation within the human transcriptome. Most of the editing sites are located in the primate-specific retrotransposed element called Alu. A large fraction of Alus are found in intronic sequences, implying extensive Alu-Alu dsRNA formation in mRNA precursors. Yet, the effect of these intronic Alus on splicing of the flanking exons is largely unknown. Here, we show that more Alus flank alternatively spliced exons than constitutively spliced ones; this is especially notable for those exons that have changed their mode of splicing from constitutive to alternative during human evolution. This implies that Alu insertions may change the mode of splicing of the flanking exons. Indeed, we demonstrate experimentally that two Alu elements that were inserted into an intron in opposite orientation undergo base-pairing, as evident by RNA editing, and affect the splicing patterns of a downstream exon, shifting it from constitutive to alternative. Our results indicate the importance of intronic Alus in influencing the splicing of flanking exons, further emphasizing the role of Alus in shaping of the human transcriptome.

  3. The four bars problem

    Science.gov (United States)

    Mauroy, Alexandre; Taslakian, Perouz; Langerman, Stefan; Jungers, Raphaël

    2016-09-01

    A four-bar linkage is a mechanism consisting of four rigid bars which are joined by their endpoints in a polygonal chain and which can rotate freely at the joints (or vertices). We assume that the linkage lies in the 2-dimensional plane so that one of the bars is held horizontally fixed. In this paper we consider the problem of reconfiguring a four-bar linkage using an operation called a pop. Given a four-bar linkage, a pop reflects a vertex across the line defined by its two adjacent vertices along the polygonal chain. Our main result shows that for certain conditions on the lengths of the bars, the neighborhood of any configuration that can be reached by smooth motion can also be reached by pops. The proof relies on the fact that pops are described by a map on the circle with an irrational number of rotation.

  4. Methods to detect faulty splices in the superconducting magnet system of the LHC

    CERN Document Server

    Bailey, R; Catalan Lasheras, N; Dahlerup-Petersen, K; Denz, R; Robles, C; Koratzinos, M; Pojer, M; Ponce, L; Saban, R; Schmidt, R; Siemko, A; Solfaroli Camillocci, M; Thiesen, H; Vergara Fernandez, A; Flora, R H; Charifoulline, Z; Bednarek, M; Górnicki, E; Jurkiewicz, P; Kapusta, P; Strait, J

    2010-01-01

    The incident of 19 September 2008 at the LHC was caused by a faulty inter-magnet splice of about 200 nΩ resistance. Cryogenic and electrical techniques have been developed to detect other abnormal splices, either between or inside the magnets. The existing quench protection system can be used to detect internal splices with R>20 nΩ. Since this system does not cover the bus between magnets, the cryogenic system is used to measure the rate of temperature rise due to ohmic heating. Accuracy of a few mK/h, corresponding to a few Watts, has been achieved, allowing detection of excess resistance, if it is more than 40 nΩ in a cryogenic subsector (two optical cells). Follow-up electrical measurements are made in regions identified by the cryogenic system. These techniques have detected two abnormal internal magnet splices of 100 nΩ and 50 nΩ respectively. In 2009, this ad hoc system will be replaced with a permanent one to monitor all splices at the nΩ level.

  5. FAST BUS Test Box (LAIKA) (Engineering Materials)

    Energy Technology Data Exchange (ETDEWEB)

    1983-01-01

    The assembly drawing AD 135-518-00-RO, and the drawings referenced thereon, provide the data and specifications for constructing the LAIKA Test Box. Some drawings are not available, although they are listed on the material lists included. The assembly is a manual tester for FAST BUS modules, both masters and slaves. FAST BUS signals are generated by means of switches or push buttons and provide the state of the bus lines by lighting LED's. The box acts as either a master or slave - depending upon the module under test. It also acts as an ATC to test the arbitration logic of a master or ATC device.

  6. Drivetrain system of an ultracapacitor electric bus

    Institute of Scientific and Technical Information of China (English)

    Kong Zhigu; Zhu Chunbo; Yang Shiyan; Cheng Shukang

    2007-01-01

    This paper proposed a design of the drivetrain system of an electric bus with ultracapacitor (UC) as the only on-board power source. The system includes three main parts, namely, UC bank, motor and the converter, vehicle management unit (VMU). Analyses results in detail on the functional design and experiments on work bench of each part were also presented, which validated the reliability of the system. Furthermore, driving results in field of the bus verified the feasibility of the design of the drivetrain system. The bus has very good dynamic performances and shows a promising applications prospect in the short and medium route buses system.

  7. Radiation-Tolerant Dual Data Bus

    Science.gov (United States)

    Kinstler, Gary A.

    2007-01-01

    An architecture, and a method of utilizing the architecture, have been proposed to enable error-free operation of a data bus that includes, and is connected to, commercial off-the-shelf (COTS) circuits and components that are inherently susceptible to singleevent upsets [SEUs (bit flips caused by impinging high-energy particles and photons)]. The architecture and method are applicable, more specifically, to data-bus circuitry based on the Institute for Electrical and Electronics Engineers (IEEE) 1394b standard for a high-speed serial bus.

  8. ParSplice, Version 1

    Energy Technology Data Exchange (ETDEWEB)

    2017-01-05

    The ParSplice code implements the Parallel Trajectory Splicing algorithm described in [1]. This method is part of the Accelerated Molecular Dynamics family of techniques developed in Los Alamos National Laboratory over the last 16 years. These methods aim at generating high-quality trajectories of ensembles of atoms in materials. ParSplice uses multiple independent replicas of the system in order to parallelize the generation of such trajectories in the time domain, enabling simulations of systems of modest size over very long timescales. ParSplice includes capabilities to store configurations of the system, to generate and distribute tasks across a large number of processors, and to harvest the results of these tasks to generate long trajectories. ParSplice is a management layer that orchestrate large number of calculations, but it does not perform the actual molecular dynamics itself; this is done by external molecular dynamics engines. [1] Danny Perez, Ekin D Cubuk, Amos Waterland, Efthimios Kaxiras, Arthur F Voter, Long-time dynamics through parallel trajectory splicing, Journal of chemical theory and computation 12, 18 (2015)

  9. Effect of periodic inflow on speed-controlled shuttle bus

    Science.gov (United States)

    Nagatani, Takashi

    2017-03-01

    We investigate the dynamic behavior of a shuttle bus controlled the speed when passengers come periodically at the origin. We propose the nonlinear-map model for the dynamics of the speed-controlled bus with the periodic inflow. The bus schedule is closely connected to the motion. The motion of the speed-controlled bus is affected by the periodic inflow. The motion of the shuttle bus depends highly on both speed control and periodic inflow. The shuttle bus displays the periodic, quasi-periodic, and chaotic motions by varying both periodic inflow and speed control. We clarify the dependence of the bus motion on both speed control and periodic inflow.

  10. Effect of speedup delay on shuttle bus schedule

    Science.gov (United States)

    Nagatani, Takashi

    2016-10-01

    We study the bus schedule in the shuttle bus transportation system controlled by speedup. The bus schedule is closely related to the dynamic motion of the bus. The motion of a shuttle bus depends on the inflow rate of passengers and the delayed speedup control. The delayed speedup control has an important effect on the dynamic motion of the bus. We present the delayed map model for the dynamics of the shuttle bus with the delayed speedup control. The bus motion changes from a stable state, through a periodic state, to a quasi-periodic state by the delayed speedup control. The return map of the tour time displays a smooth closed curve and the bus motion is quasi-periodic. The dynamic transition to the quasi-periodic motion changes greatly with the delay time. We clarify the effect of the delayed speedup control on the bus schedule.

  11. Short Nuss bar procedure

    DEFF Research Database (Denmark)

    Pilegaard, Hans Kristian

    2016-01-01

    The Nuss procedure is now the preferred operation for surgical correction of pectus excavatum (PE). It is a minimally invasive technique, whereby one to three curved metal bars are inserted behind the sternum in order to push it into a normal position. The bars are left in situ for three years...

  12. Bar-tailed

    NARCIS (Netherlands)

    Duijns, S.; Hidayati, N.A.; Piersma, T.

    2013-01-01

    Capsule Across the European wintering range Bar-tailed Godwits Limosa lapponica lapponica selected polychaete worms and especially Ragworms Hediste diversicolor, with differences between areas due to variations in prey availability.Aims To determine the diet of Bar-tailed Godwits across their winter

  13. Targeting RNA splicing for disease therapy.

    Science.gov (United States)

    Havens, Mallory A; Duelli, Dominik M; Hastings, Michelle L

    2013-01-01

    Splicing of pre-messenger RNA into mature messenger RNA is an essential step for the expression of most genes in higher eukaryotes. Defects in this process typically affect cellular function and can have pathological consequences. Many human genetic diseases are caused by mutations that cause splicing defects. Furthermore, a number of diseases are associated with splicing defects that are not attributed to overt mutations. Targeting splicing directly to correct disease-associated aberrant splicing is a logical approach to therapy. Splicing is a favorable intervention point for disease therapeutics, because it is an early step in gene expression and does not alter the genome. Significant advances have been made in the development of approaches to manipulate splicing for therapy. Splicing can be manipulated with a number of tools including antisense oligonucleotides, modified small nuclear RNAs (snRNAs), trans-splicing, and small molecule compounds, all of which have been used to increase specific alternatively spliced isoforms or to correct aberrant gene expression resulting from gene mutations that alter splicing. Here we describe clinically relevant splicing defects in disease states, the current tools used to target and alter splicing, specific mutations and diseases that are being targeted using splice-modulating approaches, and emerging therapeutics.

  14. DESIGN AND EXPERIMENT OF ELECTRIC BUS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Performance and reliability experiments on BJD-6100EV electric buses, jointly developed by Beijing Institute of Technology and Beijing Public Transport Company, are introduced. Output power model of battery pack is established and maximum output power is analyzed. A permanent magnetic direct current (PMDC) motor with enhanced windings is developed for the bus. Torque-speed characteristics of the motor are modeled and performance of electric bus is analyzed. Computational method of the range of electric bus is proposed and discussed. Experiments show that electric bus can realize design requirements. Computational methods are verified with the help of field test. It is expected that design and computation method will provide helpful reference to development of electric vehicles.

  15. Methods for Characterization of Alternative RNA Splicing

    Science.gov (United States)

    Harvey, Samuel E.; Cheng, Chonghui

    2016-01-01

    Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing “minigene” in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest. PMID:26721495

  16. Experimental determinations of the eigenmodes for composite bars made with carbon and Kevlar-carbon fibers

    Science.gov (United States)

    Miriţoiu, C. M.; Stănescu, M. M.; Burada, C. O.; Bolcu, D.; Roşca, V.

    2015-11-01

    For modal identification, the single-point excitation method has been widely used in modal tests and it consists in applying a force in a given point and recording the vibratory structure response in all interest points, including the excitation point. There will be presented the experimental recordings for the studied bars (with Kevlar-carbon or carbon fibers), the frequency response function in Cartesian and polar coordinates. By using the frequency response functions we determine the eigenparameters for each bar. We present the final panel of the eigenmodes (with the damping factors, eigenfrequencies and critical damping) for each considered bar. Using the eigenfrequency of the first determined eigenmode, the bars stiffness has been determined. The presented bars can be used in practical engineering for: car or bus body parts, planes body parts, bullet-proof vests, reinforcements for sandwich beams, and so on.

  17. Dynamic Bus Travel Time Prediction Models on Road with Multiple Bus Routes.

    Science.gov (United States)

    Bai, Cong; Peng, Zhong-Ren; Lu, Qing-Chang; Sun, Jian

    2015-01-01

    Accurate and real-time travel time information for buses can help passengers better plan their trips and minimize waiting times. A dynamic travel time prediction model for buses addressing the cases on road with multiple bus routes is proposed in this paper, based on support vector machines (SVMs) and Kalman filtering-based algorithm. In the proposed model, the well-trained SVM model predicts the baseline bus travel times from the historical bus trip data; the Kalman filtering-based dynamic algorithm can adjust bus travel times with the latest bus operation information and the estimated baseline travel times. The performance of the proposed dynamic model is validated with the real-world data on road with multiple bus routes in Shenzhen, China. The results show that the proposed dynamic model is feasible and applicable for bus travel time prediction and has the best prediction performance among all the five models proposed in the study in terms of prediction accuracy on road with multiple bus routes.

  18. Sexual Harassment on the School Bus: Supporting and Preparing Bus Drivers to Respond Appropriately

    Science.gov (United States)

    Allen, Melissa; Young, Ellie L.; Ashbaker, Betty Y.; Heaton, Emily; Parkinson, Marisa

    2003-01-01

    Sexual harassment is commonplace in schools, particularly among adolescents. Although information on this topic is typically gathered from students and teachers, this study collected information from school bus drivers. Based on feedback from 58 school bus drivers, 39 (67%) reported observing students making sexual comments or jokes. Almost half…

  19. Bullying and Aggression on the School Bus: School Bus Drivers' Observations and Suggestions

    Science.gov (United States)

    deLara, Ellen

    2008-01-01

    Every school day bus drivers are responsible for transporting children safely over many miles, yet they are rarely polled for their opinions or contributions to school safety. School bus drivers are in a unique position to inform the discussion on aggressive behavior during the school day. This exploratory study collected information from school…

  20. Short Nuss bar procedure

    Science.gov (United States)

    2016-01-01

    The Nuss procedure is now the preferred operation for surgical correction of pectus excavatum (PE). It is a minimally invasive technique, whereby one to three curved metal bars are inserted behind the sternum in order to push it into a normal position. The bars are left in situ for three years and then removed. This procedure significantly improves quality of life and, in most cases, also improves cardiac performance. Previously, the modified Ravitch procedure was used with resection of cartilage and the use of posterior support. This article details the new modified Nuss procedure, which requires the use of shorter bars than specified by the original technique. This technique facilitates the operation as the bar may be guided manually through the chest wall and no additional stabilizing sutures are necessary. PMID:27747185

  1. Sports Season, Sports Bars

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ For foreigners in Beijing, the sports bar is a special place, a place to gather for watching matches and a place to feel the familiarity of home, while for some sports enthusiasts it serves as their second home.

  2. Splice testing for LHC quadrupole magnets

    CERN Document Server

    Barzi, E; Fehér, S; Kashikhin, V V; Kerby, J S; Lamm, M J; Orris, D; Ray, G; Tartaglia, M; Zlobin, A V

    2003-01-01

    Electrical splices between NbTi Rutherford type cables need to be made for the LHC IR inner triplet quadrupoles. Splices between magnets as well as internal to the magnets are necessary. Various splice configurations, solders, and fluxes have been considered. Testing of these splices at cryogenic temperatures and at various currents has been completed. The results were satisfactory; Fermilab is capable of making excellent low resistance (<1n Omega ) solder joints for the LHC project. (4 refs).

  3. Stochastic noise in splicing machinery

    OpenAIRE

    Melamud, Eugene; Moult, John

    2009-01-01

    The number of known alternative human isoforms has been increasing steadily with the amount of available transcription data. To date, over 100 000 isoforms have been detected in EST libraries, and at least 75% of human genes have at least one alternative isoform. In this paper, we propose that most alternative splicing events are the result of noise in the splicing process. We show that the number of isoforms and their abundance can be predicted by a simple stochastic noise model that takes i...

  4. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs

    Science.gov (United States)

    Zhang, Xiao-Ou; Dong, Rui; Zhang, Yang; Zhang, Jia-Lin; Luo, Zheng; Zhang, Jun; Chen, Ling-Ling; Yang, Li

    2016-01-01

    Circular RNAs (circRNAs) derived from back-spliced exons have been widely identified as being co-expressed with their linear counterparts. A single gene locus can produce multiple circRNAs through alternative back-splice site selection and/or alternative splice site selection; however, a detailed map of alternative back-splicing/splicing in circRNAs is lacking. Here, with the upgraded CIRCexplorer2 pipeline, we systematically annotated different types of alternative back-splicing and alternative splicing events in circRNAs from various cell lines. Compared with their linear cognate RNAs, circRNAs exhibited distinct patterns of alternative back-splicing and alternative splicing. Alternative back-splice site selection was correlated with the competition of putative RNA pairs across introns that bracket alternative back-splice sites. In addition, all four basic types of alternative splicing that have been identified in the (linear) mRNA process were found within circRNAs, and many exons were predominantly spliced in circRNAs. Unexpectedly, thousands of previously unannotated exons were detected in circRNAs from the examined cell lines. Although these novel exons had similar splice site strength, they were much less conserved than known exons in sequences. Finally, both alternative back-splicing and circRNA-predominant alternative splicing were highly diverse among the examined cell lines. All of the identified alternative back-splicing and alternative splicing in circRNAs are available in the CIRCpedia database (http://www.picb.ac.cn/rnomics/circpedia). Collectively, the annotation of alternative back-splicing and alternative splicing in circRNAs provides a valuable resource for depicting the complexity of circRNA biogenesis and for studying the potential functions of circRNAs in different cells. PMID:27365365

  5. Stochastic noise in splicing machinery.

    Science.gov (United States)

    Melamud, Eugene; Moult, John

    2009-08-01

    The number of known alternative human isoforms has been increasing steadily with the amount of available transcription data. To date, over 100 000 isoforms have been detected in EST libraries, and at least 75% of human genes have at least one alternative isoform. In this paper, we propose that most alternative splicing events are the result of noise in the splicing process. We show that the number of isoforms and their abundance can be predicted by a simple stochastic noise model that takes into account two factors: the number of introns in a gene and the expression level of a gene. The results strongly support the hypothesis that most alternative splicing is a consequence of stochastic noise in the splicing machinery, and has no functional significance. The results are also consistent with error rates tuned to ensure that an adequate level of functional product is produced and to reduce the toxic effect of accumulation of misfolding proteins. Based on simulation of sampling of virtual cDNA libraries, we estimate that error rates range from 1 to 10% depending on the number of introns and the expression level of a gene.

  6. Statistical Analysis of Bus Networks in India

    CERN Document Server

    Chatterjee, Atanu; Ramadurai, Gitakrishnan

    2015-01-01

    Through the past decade the field of network science has established itself as a common ground for the cross-fertilization of exciting inter-disciplinary studies which has motivated researchers to model almost every physical system as an interacting network consisting of nodes and links. Although public transport networks such as airline and railway networks have been extensively studied, the status of bus networks still remains in obscurity. In developing countries like India, where bus networks play an important role in day-to-day commutation, it is of significant interest to analyze its topological structure and answer some of the basic questions on its evolution, growth, robustness and resiliency. In this paper, we model the bus networks of major Indian cities as graphs in \\textit{L}-space, and evaluate their various statistical properties using concepts from network science. Our analysis reveals a wide spectrum of network topology with the common underlying feature of small-world property. We observe tha...

  7. Evolutionary conservation of alternative splicing in chicken

    Science.gov (United States)

    Katyal, S.; Gao, Z.; Liu, R.-Z.; Godbout, R.

    2013-01-01

    Alternative splicing represents a source of great diversity for regulating protein expression and function. It has been estimated that one-third to two-thirds of mammalian genes are alternatively spliced. With the sequencing of the chicken genome and analysis of transcripts expressed in chicken tissues, we are now in a position to address evolutionary conservation of alternative splicing events in chicken and mammals. Here, we compare chicken and mammalian transcript sequences of 41 alternatively-spliced genes and 50 frequently accessed genes. Our results support a high frequency of splicing events in chicken, similar to that observed in mammals. PMID:17675855

  8. COMMUNICATION: Alternative splicing and genomic stability

    Science.gov (United States)

    Cahill, Kevin

    2004-06-01

    Alternative splicing allows an organism to make different proteins in different cells at different times, all from the same gene. In a cell that uses alternative splicing, the total length of all the exons is much shorter than in a cell that encodes the same set of proteins without alternative splicing. This economical use of exons makes genes more stable during reproduction and development because a genome with a shorter exon length is more resistant to harmful mutations. Genomic stability may be the reason why higher vertebrates splice alternatively. For a broad class of alternatively spliced genes, a formula is given for the increase in their stability.

  9. Alcoholism and alternative splicing of candidate genes.

    Science.gov (United States)

    Sasabe, Toshikazu; Ishiura, Shoichi

    2010-04-01

    Gene expression studies have shown that expression patterns of several genes have changed during the development of alcoholism. Gene expression is regulated not only at the level of transcription but also through alternative splicing of pre-mRNA. In this review, we discuss some of the evidence suggesting that alternative splicing of candidate genes such as DRD2 (encoding dopamine D2 receptor) may form the basis of the mechanisms underlying the pathophysiology of alcoholism. These reports suggest that aberrant expression of splice variants affects alcohol sensitivities, and alcohol consumption also regulates alternative splicing. Thus, investigations of alternative splicing are essential for understanding the molecular events underlying the development of alcoholism.

  10. GC content around splice sites affects splicing through pre-mRNA secondary structures

    Directory of Open Access Journals (Sweden)

    Chen Liang

    2011-01-01

    Full Text Available Abstract Background Alternative splicing increases protein diversity by generating multiple transcript isoforms from a single gene through different combinations of exons or through different selections of splice sites. It has been reported that RNA secondary structures are involved in alternative splicing. Here we perform a genomic study of RNA secondary structures around splice sites in humans (Homo sapiens, mice (Mus musculus, fruit flies (Drosophila melanogaster, and nematodes (Caenorhabditis elegans to further investigate this phenomenon. Results We observe that GC content around splice sites is closely associated with the splice site usage in multiple species. RNA secondary structure is the possible explanation, because the structural stability difference among alternative splice sites, constitutive splice sites, and skipped splice sites can be explained by the GC content difference. Alternative splice sites tend to be GC-enriched and exhibit more stable RNA secondary structures in all of the considered species. In humans and mice, splice sites of first exons and long exons tend to be GC-enriched and hence form more stable structures, indicating the special role of RNA secondary structures in promoter proximal splicing events and the splicing of long exons. In addition, GC-enriched exon-intron junctions tend to be overrepresented in tissue-specific alternative splice sites, indicating the functional consequence of the GC effect. Compared with regions far from splice sites and decoy splice sites, real splice sites are GC-enriched. We also found that the GC-content effect is much stronger than the nucleotide-order effect to form stable secondary structures. Conclusion All of these results indicate that GC content is related to splice site usage and it may mediate the splicing process through RNA secondary structures.

  11. Stress prevention in bus drivers: Evaluation of 13 natural experiments

    NARCIS (Netherlands)

    Kompier, M.A.J.; Aust, B.; Berg, A.M. van den; Siegrist, J.

    2000-01-01

    The research aim was to select, compare, and analyze interventions and preventive actions from international bus companies to decrease bus drivers' occupational stress and sickness absenteeism. Through networking, international surveys, and literature study, 13 "natural experiments" were identified

  12. The Bus Station Spacing Optimization Based on Game Theory

    Directory of Open Access Journals (Sweden)

    Changjiang Zheng

    2015-01-01

    Full Text Available With the development of city, the problem of traffic is becoming more and more serious. Developing public transportation has become the key to solving this problem in all countries. Based on the existing public transit network, how to improve the bus operation efficiency, and reduce the residents transit trip cost has become a simple and effective way to develop the public transportation. Bus stop spacing is an important factor affecting passengers’ travel time. How to set up bus stop spacing has become the key to reducing passengers’ travel time. According to comprehensive traffic survey, theoretical analysis, and summary of urban public transport characteristics, this paper analyzes the impact of bus stop spacing on passenger in-bus time cost and out-bus time cost and establishes in-bus time and out-bus time model. Finally, the paper gets the balance best station spacing by introducing the game theory.

  13. Secure Bus-Ticketing System using NFC

    Directory of Open Access Journals (Sweden)

    Monalisa Lopes

    2015-04-01

    Full Text Available For making the day to day life more convenient for the passengers travelling in bus some technologies can be used like Near Field Communication (NFC, Radio Frequency Identification (RFID. The proposed system is based on ticketing and identification of passengers in public transport. The system suggest a user friendly automated ticketing system which will automatically deduct the passengers fare according to the distance travelled as well as detect the passengers detail information. The system also helps to calculate the total revenue of a single bus in a day. Another important aspect is reusability, which helps use ticket multiple times as it is rechargeable.

  14. Bus training for developmentally disabled adults.

    Science.gov (United States)

    Robinson, D; Griffith, J; McComish, K; Swasbrook, K

    1984-07-01

    The effectiveness of a program combining classroom and community training in the teaching of bus-riding skills to developmentally disabled adults. These skills were taught sequentially using questions about a slide presentation, role playing, and performance in the natural environment. The experimental design was based upon the work of Neef, Iwata, and Page (1978). Test trials were conducted after each phase of training. Results showed that all subjects learned the necessary bus-riding skills and maintained their performance throughout the follow-up period of at least 1 year. The combination training method proved to be efficient and cost effective.

  15. An Efficient Segmental Bus-Invert Coding Method for Instruction Memory Data Bus Switching Reduction

    Directory of Open Access Journals (Sweden)

    Gu Ji

    2009-01-01

    Full Text Available Abstract This paper presents a bus coding methodology for the instruction memory data bus switching reduction. Compared to the existing state-of-the-art multiway partial bus-invert (MPBI coding which relies on data bit correlation, our approach is very effective in reducing the switching activity of the instruction data buses, since little bit correlation can be observed in the instruction data. Our experiments demonstrate that the proposed encoding can reduce up to 42% of switching activity, with an average of 30% reduction, while MPBI achieves just 17.6% reduction in switching activity.

  16. The RNA Splicing Response to DNA Damage.

    Science.gov (United States)

    Shkreta, Lulzim; Chabot, Benoit

    2015-10-29

    The number of factors known to participate in the DNA damage response (DDR) has expanded considerably in recent years to include splicing and alternative splicing factors. While the binding of splicing proteins and ribonucleoprotein complexes to nascent transcripts prevents genomic instability by deterring the formation of RNA/DNA duplexes, splicing factors are also recruited to, or removed from, sites of DNA damage. The first steps of the DDR promote the post-translational modification of splicing factors to affect their localization and activity, while more downstream DDR events alter their expression. Although descriptions of molecular mechanisms remain limited, an emerging trend is that DNA damage disrupts the coupling of constitutive and alternative splicing with the transcription of genes involved in DNA repair, cell-cycle control and apoptosis. A better understanding of how changes in splice site selection are integrated into the DDR may provide new avenues to combat cancer and delay aging.

  17. Titin Diversity—Alternative Splicing Gone Wild

    Directory of Open Access Journals (Sweden)

    Wei Guo

    2010-01-01

    Full Text Available Titin is an extremely large protein found in highest concentrations in heart and skeletal muscle. The single mammalian gene is expressed in multiple isoforms as a result of alternative splicing. Although titin isoform expression is controlled developmentally and in a tissue specific manner, the vast number of potential splicing pathways far exceeds those described in any other alternatively spliced gene. Over 1 million human splice pathways for a single individual can be potentially derived from the PEVK region alone. A new splicing pattern for the human cardiac N2BA isoform type has been found in which the PEVK region includes only the N2B type exons. The alterations in splicing and titin isoform expression in human heart disease provide impetus for future detailed study of the splicing mechanisms for this giant protein.

  18. Framework for Bus Rapid Transit Development and Deployment Planning

    OpenAIRE

    Miller, Mark A.; Yin, Yafeng; Balvanyos, Tunde; Ceder, Avishai

    2004-01-01

    This report presents the results of its investigation into deployment planning for bus rapid transit systems. In this study, we conducted a macro-scale examination of bus rapid transit systems from technical, operational, institutional, and planning perspectives. We then developed the theoretical foundation for a deployment planning framework for bus rapid transit systems that specifically takes into account the unique features of bus rapid transit that differ from other transit alternatives....

  19. Air quality on bus routes in Umea, Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Sandberg, H.; Forsberg, B. [Dept. of Environmental Health and Dept. of Public Health and Clinical Medicine, Umea University SE, Umea (Sweden)

    2000-07-01

    Bus traffic in some local streets with up to 650 buses per day has resulted in complaints about air pollution and suggestions from residents that the bus routes should be changed to other streets. We have studied nitrogen dioxide and benzene concentrations at five exposed sites and at two representative control sites without bus traffic, and found no general differences in air quality. However, the site and temperature adjusted concentrations fell significantly during a strike among bus drivers. (authors)

  20. Bus Routes, Bus Routes, Published in 2011, 1:2400 (1in=200ft) scale, Anne Arundel County, OIT GIS.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Field Observation information as of 2011. It is described as 'Bus...

  1. Bus Stops, This shapefile contains existing bus stops for Charlotte Area Transit System, Published in 2006, Charlotte Area Transit System.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Stops dataset, was produced all or in part from Field Survey/GPS information as of 2006. It is described as 'This shapefile contains existing bus stops for...

  2. Information management advanced development. Volume 3: Digital data bus breadboard

    Science.gov (United States)

    Gerber, C. R.

    1972-01-01

    The design, development, and evaluation of the digital data bus breadboard for the modular space station are discussed. Subjects presented are: (1) requirements summary, (2) parametric data for bus design, (3) redundancy concepts, and (4) data bus breadboard performance and interface requirements.

  3. Peptic ulcer among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1990-01-01

    As part of a survey on the work environment of bus drivers, 2045 (83%) of 2465 male bus drivers in the three major cities in Denmark in 1978 answered a postal questionnaire on health and working conditions. In order to evaluate the relative occurrence of peptic ulcer among the bus drivers, a foll...

  4. Blowers in bus systems; Einsatz von Ventilatoren in BUS-Systemen

    Energy Technology Data Exchange (ETDEWEB)

    Schneeweiss, H. [ebm - Werke GmbH und Co., Mulfingen (Germany). R und D

    2000-07-01

    Modern industrial blowers are compact and have good control characteristics. Centralized or decentralized monitoring has become a state-of-the-art technology. Bus systems make it possible to monitor and analyze component status at any time. Electronically commutated external rotor motors with bus interfaces offer a wide spectrum of applications. [German] Beim Einsatz von Ventilatoren und Geblaesen in der Industrie ist es heute Standard, dass die Geraete einen kompakten Aufbau und gute Regel- bzw. Steuereigenschaften aufweisen. Immer mehr setzt sich auch die zentrale und dezentrale Ueberwachung von Geraeten durch. Mit einem BUS-System kann der aktuelle Status jeder Komponente jederzeit abgefragt und der Zustand analysiert werden. Durch spezielle Einsatzbedingungen werden heute elektronisch kommutierte Aussenlaeufermotoren mit BUS-Schnittstellen versehen. Damit steht ein breites Anwendungsspektrum zur Verfuegung. (orig.)

  5. Spliced leader RNA trans-splicing discovered in copepods

    Science.gov (United States)

    Yang, Feifei; Xu, Donghui; Zhuang, Yunyun; Yi, Xiaoyan; Huang, Yousong; Chen, Hongju; Lin, Senjie; Campbell, David A.; Sturm, Nancy R.; Liu, Guangxing; Zhang, Huan

    2015-12-01

    Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3‧-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods.

  6. Repeater For A Digital-Communication Bus

    Science.gov (United States)

    Torres-Guzman, Esteban; Olson, Stephen; Heaps, Tim

    1993-01-01

    Digital repeater circuit designed to extend range of communication on MIL-STD-1553 bus beyond original maximum allowable length of 300 ft. Circuit provides two-way communication, one way at time, and conforms to specifications of MIL-STD-1553. Crosstalk and instability eliminated.

  7. Inrush-Current Limiter And Bus Isolator

    Science.gov (United States)

    Dolland, Carlisle R.

    1994-01-01

    Electronic circuit protects electrical power bus and source of power against excessive current demands. Circuit intended for use with power-consuming electronic system drawing pulsed currents and including capacitor bank as low-impedance secondary source from which pulsed currents drawn.

  8. SDRAM bus schedule of HDTV video decoder

    Science.gov (United States)

    Wang, Hui; He, Yan L.; Yu, Lu

    2001-12-01

    In this paper, a time division multiplexed task scheduling (TDM) is designed for HDTV video decoder is proposed. There are three tasks: to fetch decoded data from SDRAM for displaying (DIS), read the reference data from SDRAM for motion compensating (REF) and write the motion compensated data back to SDRAM (WB) on the bus. The proposed schedule is based on the novel 4 banks interlaced SDRAM storage structure which results in less overhead on read/write time. Two SDRAM of 64M bits (4Bank×512K×32bit) are used. Compared with two banks, the four banks storage strategy read/write data with 45% less time. Therefore the process data rates for those three tasks are reduced. TDM is developed by round robin scheduling and fixed slot allocating. There are both MB slot and task slot. As a result the conflicts on bus are avoided, and the buffer size is reduced 48% compared with the priority bus scheduling. Moreover, there is a compacted bus schedule for the worst case of stuffing owning to the reduced executing time on tasks. The size of buffer is reduced and the control logic is simplified.

  9. FUEL CELL BUS DEMONSTRATION IN MEXICO CITY

    Science.gov (United States)

    The report discusses the performance of a cull-size, zero-emission, Proton Exchange Membrane (PEM) fuel-cell-powered transit bus in the atmospheric environment of Mexico City. To address the air quality problems caused by vehicle emissions in Mexico City, a seminar on clean vehic...

  10. STOCHASTIC BUS DISPATCHING MODEL UNTUK OPTIMALISASI JUMLAH BUS TRANSJAKARTA KORIDOR 3

    Directory of Open Access Journals (Sweden)

    Zahedi

    2011-11-01

    Full Text Available The increasing number of road user causing traffic jam finds out a way, busway. However, in its implementation, the number of fleet available does not enough to take the passengers. It results in the an amassed of passengers at several bus stops. This leads to passenger dissatisfaction satisfaction which eventually causes Transjakarta users to reuse private cars. Definitely this situation may builds up traffic jams. Stochastic Bus Dispatching Model is used to simulate public transportation that operates regularly.

  11. Bus 54 -- Where are you? A school bus intelligent information system

    Energy Technology Data Exchange (ETDEWEB)

    Truett, L.F.; Moore, S.; Tonn, B. [Oak Ridge National Lab., TN (United States); Conley, T. [GTE Wireless, Nashville, TN (United States)

    1998-07-01

    Although major accidents involving school buses are rare (only about 0.3% of all fatal crashes since 1986 are classified as school-bus-related), even minor accidents and breakdowns cause a great deal of parental anxiety. The objective of this research is to design an efficient, cost-effective, accurate, and secure system that will track individual school buses and communicate appropriate information to the school system`s central administration unit, to the school transportation administrator, and to parents of children on the bus. The greatest benefit of the proposed information system is that parents and officials can always know the location and condition (these conditions would vary depending on the needs of a particular school system) of the school buses. In case of an accident or mechanical problem, when emergency crews are needed, they can be dispatched almost immediately with a good understanding of the problem and the exact location of the bus. In addition to being able to track the bus while the child is on board, parents will be able to determine the location of their child`s bus prior to its arrival in the morning in order to prevent the child from needing to wait outside in inclement weather. The information available to parents can also be expanded to include maps of limited routes (e.g., snow routes). Basically, the Bus 54 concept consists of a bus component and a central data processing unit. Each bus will be outfitted with a global positioning satellite (GPS) device, a wireless communication device, and wireless data communication service. The central data processing unit will receive and process information from the buses and provide information access to parents and officials via an Internet link.

  12. Advanced eGovernment Information Service Bus (eGov-Bus

    Directory of Open Access Journals (Sweden)

    2007-01-01

    Full Text Available The eGov-Bus project provides citizens and businesses with improved access to virtual public services, which are based on existing national eGovernment Web services and which support cross-border life events. Requirements and specific rules of these life events are considered, and personalization of user preferences is supported. eGov-Bus is based on adaptable process management technologies, allowing for virtual services which are dynamically combined from existing national eGovernment services. In this way, a comprehensive workflow process is set up, allowing for service-level agreements, an audit trail and explanation of the process to the end user. The eGov-Bus process engine operates on top of a virtual repository, providing a high-level semantic view of information retrieved from heterogeneous information sources, such as eGovernment Web services. Further, eGov-Bus relies on a security framework to ensure all high-level security requirements are met. The eGov-Bus architecture is business oriented, it focuses on Service Oriented Architecture (SOA concepts, asynchronously combining Web services and providing a Service Bus.

  13. Error bars in experimental biology.

    Science.gov (United States)

    Cumming, Geoff; Fidler, Fiona; Vaux, David L

    2007-04-09

    Error bars commonly appear in figures in publications, but experimental biologists are often unsure how they should be used and interpreted. In this article we illustrate some basic features of error bars and explain how they can help communicate data and assist correct interpretation. Error bars may show confidence intervals, standard errors, standard deviations, or other quantities. Different types of error bars give quite different information, and so figure legends must make clear what error bars represent. We suggest eight simple rules to assist with effective use and interpretation of error bars.

  14. Gaseous Structures in Barred Galaxies: Effects of the Bar Strength

    CERN Document Server

    Kim, Woong-Tae; Kim, Yonghwi

    2012-01-01

    Using hydrodynamic simulations, we investigate the physical properties of gaseous substructures in barred galaxies and their relationships with the bar strength. The gaseous medium is assumed to be isothermal and unmagnetized. The bar potential is modeled as a Ferrers prolate with index n. To explore situations with differing bar strength, we vary the bar mass fbar relative to the spheroidal component as well as its aspect ratio. We derive expressions as functions of fbar and the aspect ratio for the bar strength Qb and the radius r(Qb) where the maximum bar torque occurs. When applied to observations, these expressions suggest that bars in real galaxies are most likely to have fbar=0.25-0.5 and n0.2 and self-gravity is included.

  15. Raising the Bar (3)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    2016-01-01

    This editorial summarizes and comments on the papers published in issue 11(3) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper proposes spatial and a-spatial indicators to describe the networks of airline companies around the world. The second pap

  16. Highest Oxygen Bar

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The world’s highest altitude Lhalu Wetland in Tibet is rebounding from past environmental damage In Lhasa, where the oxygen content is just 60 percent of that of the plain area, a place known as the "natural oxygen bar"is highly prized by residents.

  17. Raising the bar (2)

    NARCIS (Netherlands)

    Elhorst, P.; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    2016-01-01

    In this editorial we summarise and comment on the papers published in issue 11.2 so as to raise the bar in applied spatial economic research and highlight new trends. The first paper analyses which regions in Europe were resilient to the great Recession and which ones were not. The second and the th

  18. Splicing-related genes are alternatively spliced upon changes in ambient temperatures in plants

    Science.gov (United States)

    Bucher, Johan; Lammers, Michiel; Busscher-Lange, Jacqueline; Bonnema, Guusje; Rodenburg, Nicole; Proveniers, Marcel C. G.; Angenent, Gerco C.

    2017-01-01

    Plants adjust their development and architecture to small variations in ambient temperature. In a time in which temperatures are rising world-wide, the mechanism by which plants are able to sense temperature fluctuations and adapt to it, is becoming of special interest. By performing RNA-sequencing on two Arabidopsis accession and one Brassica species exposed to temperature alterations, we showed that alternative splicing is an important mechanism in ambient temperature sensing and adaptation. We found that amongst the differentially alternatively spliced genes, splicing related genes are enriched, suggesting that the splicing machinery itself is targeted for alternative splicing when temperature changes. Moreover, we showed that many different components of the splicing machinery are targeted for ambient temperature regulated alternative splicing. Mutant analysis of a splicing related gene that was differentially spliced in two of the genotypes showed an altered flowering time response to different temperatures. We propose a two-step mechanism where temperature directly influences alternative splicing of the splicing machinery genes, followed by a second step where the altered splicing machinery affects splicing of downstream genes involved in the adaptation to altered temperatures. PMID:28257507

  19. Bus accident severity and passenger injury: evidence from Denmark

    DEFF Research Database (Denmark)

    Prato, Carlo Giacomo; Kaplan, Sigal

    2014-01-01

    examining occurrence of injury to bus passengers. Results Bus accident severity is positively related to (i) the involvement of vulnerable road users, (ii) high speed limits, (iii) night hours, (iv) elderly drivers of the third party involved, and (v) bus drivers and other drivers crossing in yellow or red...... principle of sustainable transit and advance the vision “every accident is one too many”. Methods Bus accident data were retrieved from the national accident database for the period 2002–2011. A generalized ordered logit model allows analyzing bus accident severity and a logistic regression enables...

  20. Regenerative polymeric bus architecture for board-level optical interconnects.

    Science.gov (United States)

    Bamiedakis, N; Hashim, A; Penty, R V; White, I H

    2012-05-21

    A scalable multi-channel optical regenerative bus architecture based on the use of polymer waveguides is presented for the first time. The architecture offers high-speed interconnection between electrical cards allowing regenerative bus extension with multiple segments and therefore connection of an arbitrary number of cards onto the bus. In a proof-of-principle demonstration, a 4-channel 3-card polymeric bus module is designed and fabricated on standard FR4 substrates. Low insertion losses (≤ -15 dB) and low crosstalk values (bus modules using a prototype 3R regenerator.

  1. Energy efficiency of different bus subsystems in Belgrade public transport

    Directory of Open Access Journals (Sweden)

    Mišanović Slobodan M.

    2015-01-01

    Full Text Available Research in this paper comprised experimental determination of energy efficiency of different bus subsystems (diesel bus, trolleybus and fully electric bus on chosen public transport route in Belgrade. Experimental measuring of energy efficiency of each bus type has been done based on the analysis of parameters of vehicle driving cycles between stops. Results of this analysis were basis for development of theoretical simulation model of energy efficiency. The model was latter compared with the results of simulation done by "Solaris bus & Coach" company for the chosen electric bus route. Based on demonstrated simulation, characteristics of electric bus batteries were defined, the method and dynamic of their recharge was suggested, as well as choice for other aggregates for drive system and technical characteristics for the electric buses were suggested.

  2. Integer programming model for optimizing bus timetable using genetic algorithm

    Science.gov (United States)

    Wihartiko, F. D.; Buono, A.; Silalahi, B. P.

    2017-01-01

    Bus timetable gave an information for passengers to ensure the availability of bus services. Timetable optimal condition happened when bus trips frequency could adapt and suit with passenger demand. In the peak time, the number of bus trips would be larger than the off-peak time. If the number of bus trips were more frequent than the optimal condition, it would make a high operating cost for bus operator. Conversely, if the number of trip was less than optimal condition, it would make a bad quality service for passengers. In this paper, the bus timetabling problem would be solved by integer programming model with modified genetic algorithm. Modification was placed in the chromosomes design, initial population recovery technique, chromosomes reconstruction and chromosomes extermination on specific generation. The result of this model gave the optimal solution with accuracy 99.1%.

  3. Multiple bars and secular evolution

    Science.gov (United States)

    Shen, Juntai

    2015-03-01

    Bars are the most important driver of secular evolution. A significant fraction of barred galaxies also harbor small secondary bars. Secondary bars are visible even in near-infrared images, so they are not just dusty and blue, but stellar features (Erwin & Sparke 2002). Since they are quite common, secondary bars are probably long-lived stellar features. The random relative orientation of the two bars indicates that they are dynamically decoupled with different pattern speeds (Buta & Crocker 1993). Corsini et al. (2003) presented conclusive direct kinematic evidence for a decoupled secondary bar in NGC 2950. Dynamically decoupled secondary bars have long been hypothesized to be a mechanism to drive gas past the ILR of primary bars to feed active galactic nuclei (Shlosman et al. 1989). However, the dynamics of secondary bars are still not well understood, and it is still unclear what role secondary bars play in the AGN fueling process. Numerical simulations offer the best approach to understanding double-barred systems. Decoupled secondary bar in the earlier gaseous simulations only last a short time (body simulations are preferred to further our understanding of double-barred systems. Debattista & Shen (2007) and Shen & Debattista (2009) managed to form long-lived double-barred systems with purely collisionless simulations, where a pre-existing rotating pseudo-bulge is introduced initially. The shape and size of secondary bars in the models are comparable to observed ones. They found that the rotation of the two bars is not rigid. The amplitude and pattern speed of the secondary bars oscillate as they rotate through their primary counterparts. Although the secondary bar rotates faster than the primary bar in this model, the stellar velocity field in the central region only shows a weakly twisted kinematic minor axis. Recently more simulations of double-barred galaxies with simpler initial conditions are explored (Du, Shen & Debattista 2014). We expect that the new

  4. Error bars in experimental biology

    OpenAIRE

    2007-01-01

    Error bars commonly appear in figures in publications, but experimental biologists are often unsure how they should be used and interpreted. In this article we illustrate some basic features of error bars and explain how they can help communicate data and assist correct interpretation. Error bars may show confidence intervals, standard errors, standard deviations, or other quantities. Different types of error bars give quite different information, and so figure legends must make clear what er...

  5. Alternative Spliced Transcripts as Cancer Markers

    Directory of Open Access Journals (Sweden)

    Otavia L. Caballero

    2001-01-01

    Full Text Available Eukaryotic mRNAs are transcribed as precursors containing their intronic sequences. These are subsequently excised and the exons are spliced together to form mature mRNAs. This process can lead to transcript diversification through the phenomenon of alternative splicing. Alternative splicing can take the form of one or more skipped exons, variable position of intron splicing or intron retention. The effect of alternative splicing in expanding protein repertoire might partially underlie the apparent discrepancy between gene number and the complexity of higher eukaryotes. It is likely that more than 50% form. Many cancer-associated genes, such as CD44 and WT1 are alternatively spliced. Variation of the splicing process occurs during tumor progression and may play a major role in tumorigenesis. Furthermore, alternatively spliced transcripts may be extremely useful as cancer markers, since it appears likely that there may be striking contrasts in usage of alternatively spliced transcript variants between normal and tumor tissue than in alterations in the general levels of gene expression.

  6. Observations of offshore bar decay

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian

    2010-01-01

    Long-term, net offshore bar migration is a common occurrence on many multiple-barred beaches. The first stage of the process involves the generation of a longshore bar close to the shoreline that oscillates about a mean position for some time, followed by a stage of net offshore migration across...

  7. Membrane biology: fission behind BARs.

    Science.gov (United States)

    Haucke, Volker

    2012-06-05

    Membrane bending is accomplished in part by amphipathic helix insertion into the bilayer and the assembly of BAR domain scaffolds preparing the membrane for fission. Two recent studies highlight the roles of amphipathic helices and BAR scaffolds in membrane fission and establish the structural basis of membrane bending by the N-BAR protein endophilin.

  8. Regulation of splicing factors by alternative splicing and NMD is conserved between kingdoms yet evolutionarily flexible.

    Science.gov (United States)

    Lareau, Liana F; Brenner, Steven E

    2015-04-01

    Ultraconserved elements, unusually long regions of perfect sequence identity, are found in genes encoding numerous RNA-binding proteins including arginine-serine rich (SR) splicing factors. Expression of these genes is regulated via alternative splicing of the ultraconserved regions to yield mRNAs that are degraded by nonsense-mediated mRNA decay (NMD), a process termed unproductive splicing (Lareau et al. 2007; Ni et al. 2007). As all human SR genes are affected by alternative splicing and NMD, one might expect this regulation to have originated in an early SR gene and persisted as duplications expanded the SR family. But in fact, unproductive splicing of most human SR genes arose independently (Lareau et al. 2007). This paradox led us to investigate the origin and proliferation of unproductive splicing in SR genes. We demonstrate that unproductive splicing of the splicing factor SRSF5 (SRp40) is conserved among all animals and even observed in fungi; this is a rare example of alternative splicing conserved between kingdoms, yet its effect is to trigger mRNA degradation. As the gene duplicated, the ancient unproductive splicing was lost in paralogs, and distinct unproductive splicing evolved rapidly and repeatedly to take its place. SR genes have consistently employed unproductive splicing, and while it is exceptionally conserved in some of these genes, turnover in specific events among paralogs shows flexible means to the same regulatory end.

  9. The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin.

    Science.gov (United States)

    Collins, Richard A; Stajich, Jason E; Field, Deborah J; Olive, Joan E; DeAbreu, Diane M

    2015-05-01

    When we expressed a small (0.9 kb) nonprotein-coding transcript derived from the mitochondrial VS plasmid in the nucleus of Neurospora we found that it was efficiently spliced at one or more of eight 5' splice sites and ten 3' splice sites, which are present apparently by chance in the sequence. Further experimental and bioinformatic analyses of other mitochondrial plasmids, random sequences, and natural nuclear genes in Neurospora and other fungi indicate that fungal spliceosomes recognize a wide range of 5' splice site and branchpoint sequences and predict introns to be present at high frequency in random sequence. In contrast, analysis of intronless fungal nuclear genes indicates that branchpoint, 5' splice site and 3' splice site consensus sequences are underrepresented compared with random sequences. This underrepresentation of splicing signals is sufficient to deplete the nuclear genome of splice sites at locations that do not comprise biologically relevant introns. Thus, the splicing machinery can recognize a wide range of splicing signal sequences, but splicing still occurs with great accuracy, not because the splicing machinery distinguishes correct from incorrect introns, but because incorrect introns are substantially depleted from the genome.

  10. Dark Matter Trapping by Stellar Bars: The Shadow Bar

    Science.gov (United States)

    Petersen, Michael S.; Weinberg, Martin D.; Katz, Neal

    2016-09-01

    We investigate the complex interactions between the stellar disc and the dark-matter halo during bar formation and evolution using N-body simulations with fine temporal resolution and optimally chosen spatial resolution. We find that the forming stellar bar traps dark matter in the vicinity of the stellar bar into bar-supporting orbits. We call this feature the shadow bar. The shadow bar modifies both the location and magnitude of the angular momentum transfer between the disc and dark matter halo and adds 10 per cent to the mass of the stellar bar over 4 Gyr. The shadow bar is potentially observable by its density and velocity signature in spheroid stars and by direct dark matter detection experiments. Numerical tests demonstrate that the shadow bar can diminish the rate of angular momentum transport from the bar to the dark matter halo by more than a factor of three over the rate predicted by dynamical friction with an untrapped dark halo, and thus provides a possible physical explanation for the observed prevalence of fast bars in nature.

  11. Dark Matter Trapping by Stellar Bars: The Shadow Bar

    CERN Document Server

    Petersen, Michael S; Katz, Neal

    2016-01-01

    We investigate the complex interactions between the stellar disc and the dark-matter halo during bar formation and evolution using N-body simulations with fine temporal resolution and optimally chosen spatial resolution. We find that the forming stellar bar traps dark matter in the vicinity of the stellar bar into bar-supporting orbits. We call this feature the shadow bar. The shadow bar modifies both the location and magnitude of the angular momentum transfer between the disc and dark matter halo and adds 10 per cent to the mass of the stellar bar over 4 Gyr. The shadow bar is potentially observable by its density and velocity signature in spheroid stars and by direct dark matter detection experiments. Numerical tests demonstrate that the shadow bar can diminish the rate of angular momentum transport from the bar to the dark matter halo by more than a factor of three over the naive dynamical friction prediction, and thus provides a possible physical explanation for the observed prevalence of fast bars in nat...

  12. Dark matter trapping by stellar bars: the shadow bar

    Science.gov (United States)

    Petersen, Michael S.; Weinberg, Martin D.; Katz, Neal

    2016-12-01

    We investigate the complex interactions between the stellar disc and the dark-matter halo during bar formation and evolution using N-body simulations with fine temporal resolution and optimally chosen spatial resolution. We find that the forming stellar bar traps dark matter in the vicinity of the stellar bar into bar-supporting orbits. We call this feature the shadow bar. The shadow bar modifies both the location and magnitude of the angular momentum transfer between the disc and dark matter halo and adds 10 per cent to the mass of the stellar bar over 4 Gyr. The shadow bar is potentially observable by its density and velocity signature in spheroid stars and by direct dark matter detection experiments. Numerical tests demonstrate that the shadow bar can diminish the rate of angular momentum transport from the bar to the dark matter halo by more than a factor of 3 over the rate predicted by dynamical friction with an untrapped dark halo, and thus provides a possible physical explanation for the observed prevalence of fast bars in nature.

  13. The bar instability revisited

    CERN Document Server

    Chiodi, Filippo; Claudin, Philippe

    2012-01-01

    The river bar instability is revisited, using a hydrodynamical model based on Reynolds averaged Navier-Stokes equations. The results are contrasted with the standard analysis based on shallow water Saint-Venant equations. We first show that the stability of both transverse modes (ripples) and of small wavelength inclined modes (bars) predicted by the Saint-Venant approach are artefacts of this hydrodynamical approximation. When using a more reliable hydrodynamical model, the dispersion relation does not present any maximum of the growth rate when the sediment transport is assumed to be locally saturated. The analysis therefore reveals the fundamental importance of the relaxation of sediment transport towards equilibrium as it it is responsible for the stabilisation of small wavelength modes. This dynamical mechanism is characterised by the saturation number, defined as the ratio of the saturation length to the water depth Lsat/H. This dimensionless number controls the transition from ripples (transverse patte...

  14. Breaking through the Bar

    Science.gov (United States)

    Gray, Katti

    2011-01-01

    Howard University School of Law had a problem, and school officials knew it. Over a 20-year period, 40 percent of its graduates who took the Maryland bar exam failed it on their first try. During the next 24 months--the time frame required to determine its "eventual pass rate"--almost 90 percent of the students did pass. What they did not know was…

  15. Strained graphene Hall bar

    Science.gov (United States)

    Milovanović, S. P.; Peeters, F. M.

    2017-02-01

    The effects of strain, induced by a Gaussian bump, on the magnetic field dependent transport properties of a graphene Hall bar are investigated. The numerical simulations are performed using both classical and quantum mechanical transport theory and we found that both approaches exhibit similar characteristic features. The effects of the Gaussian bump are manifested by a decrease of the bend resistance, R B, around zero-magnetic field and the occurrence of side-peaks in R B. These features are explained as a consequence of bump-assisted scattering of electrons towards different terminals of the Hall bar. Using these features we are able to give an estimate of the size of the bump. Additional oscillations in R B are found in the quantum description that are due to the population/depopulation of Landau levels. The bump has a minor influence on the Hall resistance even for very high values of the pseudo-magnetic field. When the bump is placed outside the center of the Hall bar valley polarized electrons can be collected in the leads.

  16. SplicingTypesAnno: annotating and quantifying alternative splicing events for RNA-Seq data.

    Science.gov (United States)

    Sun, Xiaoyong; Zuo, Fenghua; Ru, Yuanbin; Guo, Jiqiang; Yan, Xiaoyan; Sablok, Gaurav

    2015-04-01

    Alternative splicing plays a key role in the regulation of the central dogma. Four major types of alternative splicing have been classified as intron retention, exon skipping, alternative 5 splice sites or alternative donor sites, and alternative 3 splice sites or alternative acceptor sites. A few algorithms have been developed to detect splice junctions from RNA-Seq reads. However, there are few tools targeting at the major alternative splicing types at the exon/intron level. This type of analysis may reveal subtle, yet important events of alternative splicing, and thus help gain deeper understanding of the mechanism of alternative splicing. This paper describes a user-friendly R package, extracting, annotating and analyzing alternative splicing types for sequence alignment files from RNA-Seq. SplicingTypesAnno can: (1) provide annotation for major alternative splicing at exon/intron level. By comparing the annotation from GTF/GFF file, it identifies the novel alternative splicing sites; (2) offer a convenient two-level analysis: genome-scale annotation for users with high performance computing environment, and gene-scale annotation for users with personal computers; (3) generate a user-friendly web report and additional BED files for IGV visualization. SplicingTypesAnno is a user-friendly R package for extracting, annotating and analyzing alternative splicing types at exon/intron level for sequence alignment files from RNA-Seq. It is publically available at https://sourceforge.net/projects/splicingtypes/files/ or http://genome.sdau.edu.cn/research/software/SplicingTypesAnno.html.

  17. Research of intelligent bus coin box

    Science.gov (United States)

    Xin, Shihao

    2017-03-01

    In the energy-saving emission reduction of the social context, in response to low-carbon travel, buses become the majority of people choose. We have designed this sorting machine for the present situation that the bus company has received a large amount of mixed zero coins and employed a large amount of manpower to sort out and lower the efficiency. Its function is to separate the coins and notes mixed, and the coins sort storage, the display shows the value of the received coins, so that the whole mechanized inventory classification, reduce the cost of clearing up and improve the efficiency of zero cash recycling, use Simple mechanical principles for classification, to be efficient, accurate and practical. Really meet the current city bus companies, commerce and banking and other industries in order to zero notes, zero coins in the actual demand. The size and specification of this machine are designed according to the size of the bus coin box. It is suitable for almost all buses. It can be installed in the coin box directly, real-time sorting and real-time counting. The difficulty of clearing change.

  18. Brazilian hybrid electric fuel cell bus

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, P.E.V.; Carreira, E.S. [Coppe-Federal Univ. of Rio de Janeiro (Brazil). Hydrogen Lab.

    2010-07-01

    The first prototype of a hybrid electric fuel cell bus developed with Brazilian technology is unveiled. It is a 12 m urban-type, low-floor, air-conditioned bus that possesses three doors, air suspension, 29 seats and reversible wheelchair site. The bus body was built based on a double-deck type monoblock vehicle that is able to sustain important load on its roof. This allowed positioning of the type 3 hydrogen tanks and the low weight traction batteries on the roof of the vehicles without dynamic stabilization problems. A novel hybrid energy configuration was designed in such a way that the low-power (77 kWe) fuel cell works on steady-state operation mode, not responding directly to the traction motor load demand. The rate of kinetic energy regeneration upon breaking was optimized by the use of an electric hybrid system with predominance of batteries and also by utilizing supercapacitors. The electric-electronic devices and the security control softwares for the auxiliary and traction systems were developed in-house. The innovative hybrid-electric traction system configuration led to the possibility to decrease the fuel cell power, with positive impact on weight and system volume reduction, as well as to significantly decrease the hydrogen consumption. (orig.)

  19. Barred Galaxies: an Observer's Perspective

    CERN Document Server

    Gadotti, Dimitri A

    2008-01-01

    I review both well established and more recent findings on the properties of bars, and their host galaxies, stemming from photometric and spectroscopic observations, and discuss how these findings can be understood in terms of a global picture of the formation and evolution of bars, keeping a connection with theoretical developments. In particular, I show the results of a detailed structural analysis of ~ 300 barred galaxies in the Sloan Digital Sky Survey, providing physical quantities, such as bar length, ellipticity and boxyness, and bar-to-total luminosity ratio, that can either be used as a solid basis on which realistic models can be built, or be compared against more fundamental theoretical results. I also show correlations that indicate that bars grow longer, thinner and stronger with dynamical age, and that the growth of bars and bulges is connected. Finally, I briefly discuss open questions and possible directions for future research.

  20. Quench propagation and detection in the superconducting bus-bars of the ATLAS magnets

    CERN Document Server

    Dudarev, A; ten Kate, H H J; Sbrissa, E; Yamamoto, A; Baynham, D Elwyn; Courthold, M J D; Lesmond, C

    2000-01-01

    The ATLAS superconducting magnet system comprising Barrel (BT) and End-Cap Toroids (ECT) and also Central Solenoid (CS) will store more than 1.5 GJ of magnetic energy. The magnet system will have many superconducting busbars, a few meters long each, running from the current leads to Central Solenoid and Toroids as well as between the coils of each Toroid. Quench development in the busbars, i.e., the normal zone propagation process along the busbar superconductors, is slow and exhibits very low voltages. Therefore, its timely and appropriate detection represents a real challenge. The temperature evolution in the busbars under quench is of primary importance. Conservative calculations of the temperature were performed for all the magnets. Also, a simple and effective method to detect a normal zone in a busbar is presented. A thin superconducting wire, whose normal resistance can be easily detected, is placed in a good thermal contact to busbar. Thus, the wire can operate as straightforward and low-noise quench-...

  1. Aberrant RNA splicing in cancer; expression changes and driver mutations of splicing factor genes.

    Science.gov (United States)

    Sveen, A; Kilpinen, S; Ruusulehto, A; Lothe, R A; Skotheim, R I

    2016-05-12

    Alternative splicing is a widespread process contributing to structural transcript variation and proteome diversity. In cancer, the splicing process is commonly disrupted, resulting in both functional and non-functional end-products. Cancer-specific splicing events are known to contribute to disease progression; however, the dysregulated splicing patterns found on a genome-wide scale have until recently been less well-studied. In this review, we provide an overview of aberrant RNA splicing and its regulation in cancer. We then focus on the executors of the splicing process. Based on a comprehensive catalog of splicing factor encoding genes and analyses of available gene expression and somatic mutation data, we identify cancer-associated patterns of dysregulation. Splicing factor genes are shown to be significantly differentially expressed between cancer and corresponding normal samples, and to have reduced inter-individual expression variation in cancer. Furthermore, we identify enrichment of predicted cancer-critical genes among the splicing factors. In addition to previously described oncogenic splicing factor genes, we propose 24 novel cancer-critical splicing factors predicted from somatic mutations.

  2. Power-optimal encoding for low-power address bus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper presented a novel bus encoding method to reduce the switching activity on address buses and hence reduce power dissipation. Dynamic-sorting encoding (DSE) method reduces the power dissipation of address bus based on the dynamic reordering of the modified offset address bus lines. This method reorders the ten least significant bits of offset address according to the range of offset address, and the optimal sorting pattern is transmitted through the high bits of address bus without the need for redundant bus lines. The experimental results using an instruction set simulator and SPEC2000 benchmarks show that DSE method can reduce signal transitions on the address bus by 88.2%, and the actual overhead of the encoder circuit is estimated after encoder is designed and synthesized in 0.18-μm CMOS technology. The results show that DSE method outperforms the low-power encoding schemes presented in the past.

  3. Osmotic regulation of transcription in Lactococcus lactis: ionic strength-dependent binding of the BusR repressor to the busA promoter.

    Science.gov (United States)

    Romeo, Yves; Bouvier, Jean; Gutierrez, Claude

    2007-07-24

    The busA locus of Lactococcus lactis encodes a glycine betaine uptake system. At low osmolarity, the transcription of busA is repressed by the BusR protein, which is responsible for the osmotic inducibility of the busA promoter (busAp). In this work, we investigated the mechanism of the osmo-dependent repression by BusR. We found that BusR binding to the busA promoter is dependent on the ionic strength in vitro. Using a BusR derivative carrying a phosphorylation site and the Escherichia coli RNA polymerase holoenzyme, we showed that these proteins are able to form a stable ternary complex by both binding to the same busAp fragment. The association/dissociation of BusR to the RNA polymerase-busAp complex is strictly correlated to the surrounding ionic strength. Together, these results suggest that during growth at low osmolarity BusR represses transcription from busAp at a step further the recruitment of the RNA polymerase. At high osmolarity, an elevated cytoplasmic ionic strength would dissociate BusR from busAp, resulting in the osmotic induction of the busA operon.

  4. Alcoholism and Alternative Splicing of Candidate Genes

    Directory of Open Access Journals (Sweden)

    Toshikazu Sasabe

    2010-03-01

    Full Text Available Gene expression studies have shown that expression patterns of several genes have changed during the development of alcoholism. Gene expression is regulated not only at the level of transcription but also through alternative splicing of pre-mRNA. In this review, we discuss some of the evidence suggesting that alternative splicing of candidate genes such as DRD2 (encoding dopamine D2 receptor may form the basis of the mechanisms underlying the pathophysiology of alcoholism. These reports suggest that aberrant expression of splice variants affects alcohol sensitivities, and alcohol consumption also regulates alternative splicing. Thus, investigations of alternative splicing are essential for understanding the molecular events underlying the development of alcoholism.

  5. Emission inventory estimation of an intercity bus terminal.

    Science.gov (United States)

    Qiu, Zhaowen; Li, Xiaoxia; Hao, Yanzhao; Deng, Shunxi; Gao, H Oliver

    2016-06-01

    Intercity bus terminals are hotspots of air pollution due to concentrated activities of diesel buses. In order to evaluate the bus terminals' impact on air quality, it is necessary to estimate the associated mobile emission inventories. Since the vehicles' operating condition at the bus terminal varies significantly, conventional calculation of the emissions based on average emission factors suffers the loss of accuracy. In this study, we examined a typical intercity bus terminal-the Southern City Bus Station of Xi'an, China-using a multi-scale emission model-(US EPA's MOVES model)-to quantity the vehicle emission inventory. A representative operating cycle for buses within the station is constructed. The emission inventory was then estimated using detailed inputs including vehicle ages, operating speeds, operating schedules, and operating mode distribution, as well as meteorological data (temperature and humidity). Five functional areas (bus yard, platforms, disembarking area, bus travel routes within the station, and bus entrance/exit routes) at the terminal were identified, and the bus operation cycle was established using the micro-trip cycle construction method. Results of our case study showed that switching to compressed natural gas (CNG) from diesel fuel could reduce PM2.5 and CO emissions by 85.64 and 6.21 %, respectively, in the microenvironment of the bus terminal. When CNG is used, tail pipe exhaust PM2.5 emission is significantly reduced, even less than brake wear PM2.5. The estimated bus operating cycles can also offer researchers and policy makers important information for emission evaluation in the planning and design of any typical intercity bus terminals of a similar scale.

  6. Pipelined asynchronous time-division multiplexing optical bus

    Science.gov (United States)

    Zheng, S. Q.; Li, Yueming

    1997-12-01

    We propose a pipelined asynchronous time-division multiplexing optical bus. Such a bus can use one of two hardwared priority schemes: the linear priority scheme and the round-robin priority scheme. Our simulation results show that the performance of the proposed bus is significantly better than the performances of known pipelined synchronous time-division multiplexing optical buses. The possibilities of using our buses to construct multichannel switches and multidimensional processor arrays are also discussed.

  7. A 3D bus interconnect for network line cards

    OpenAIRE

    Engel, J; Kocak, T

    2004-01-01

    In this paper, we propose a 3D bus architecture as a processor-memory interconnection system to increase the throughput of the memory system currently used on line cards. The 3D bus architecture allows multiple processing elements on a line card to access a shared memory. The main advantage of the proposed architecture is to increase the network processor off-chip memory bandwidth while diminishing the latency otherwise caused by the single bus competition.

  8. PNEUMATIC - LOGIC SYNTHESIS OF BUS DOOR FROM ASPECT OF AUTOMATION

    Directory of Open Access Journals (Sweden)

    Slobodan Stefanovic

    2015-01-01

    Full Text Available The paper presents a logic synthesis procedure pneumatic control of the door of the bus for suburban traffic. Minimization of pneumatic control is determined graphically by Weitz – Carnot method. It also shows all pneumatic components belonging to installations used for opening and closing the door of the bus. Finally it is shown the basic maintenance of pneumatic system of the bus door.

  9. The Impact of Corrosion on the Mechanical Behavior of Welded Splices of Reinforcing Steel S400 and B500c

    Science.gov (United States)

    Apostolopoulos, Ch. Alk.; Michalopoulos, D.; Dimitrov, L.

    2008-02-01

    The reinforcing steel, used in concrete structures, when corroded causes reduction of the strength properties and especially drastic reduction of ductility. Steel corrosion constitutes an important factor of progressive devaluation of its mechanical properties and serious reduction of the integrity of structures. The problem becomes more evident specifically for structures near coastal areas where salt corrosion is predominant. Reinforced concrete columns and beams are quite often extended by welding new steel reinforcement to the already corroded existing steel. In the present article the impact of corrosion on the mechanical properties of welded splices of reinforcing Steel S400 and B500c is examined. An experimental investigation was conducted and tensile and compressive results are presented for welded precorroded S400 and noncorroded B500c steel splices. The mechanical behavior of welded splices in tension are different in compression and depend strongly on the level of corrosion of the S400 bars.

  10. Spliced

    DEFF Research Database (Denmark)

    Addison, Courtney Page

    2016-01-01

    Human gene therapy (HGT) aims to cure disease by inserting or editing the DNA of patients with genetic conditions. Since foundational genetic techniques came into use in the 1970s, the field has developed to the point that now three therapies have market approval, and over 1800 clinical trials have...... been initiated. In this article I present a brief history of HGT, showing how the ethical and practical viability of the field was achieved by key scientific and regulatory actors. These parties carefully articulated gene therapy’s scope, limiting it to therapeutic interventions on somatic cells......, and cultivated alliances and divisions that bolstered the field’s legitimacy. At times these measures faltered, and then practitioners and sometimes patients would invoke an ethical imperative, posing gene therapy as the best solution to life and death problems. I suggest that we consider how boundary...

  11. Bus coordination holding control for transit hubs under APTS

    Institute of Scientific and Technical Information of China (English)

    TENG Jing; ZHAO Ming

    2009-01-01

    To increase the passenger transferring efficiency, the bus coordination holding control for transit hubs,hich is as an important dynamic dispatching method for improving the service level of transit hubs, was studied in the framework of bus coordination dispatching mode. Firstly, the bus coordination holding control flow was studied based on Advanced Public Transportation Systems (APTS) environment. Then a control model was presented to optimize the bus vehicle holding time, and a genetic algorithm was designed as the solving method. In the end, an example was given to illustrate the effectiveness of the control strategy and the algorithm.

  12. Model Passengers’ Travel Time for Conventional Bus Stop

    Directory of Open Access Journals (Sweden)

    Guangzhao Xin

    2014-01-01

    Full Text Available Limited number of berths can result in a subsequent bus stopping at the upstream of a bus stop when all berths are occupied. When this traffic phenomenon occurs, passengers waiting on the platform usually prefer walking to the stopped bus, which leads to additional walking time before boarding the bus. Therefore, passengers’ travel time consumed at a bus stop is divided into waiting time, additional walking time, and boarding time. This paper proposed a mathematical model for analyzing passengers’ travel time at conventional bus stop based on theory of stochastic service system. Field-measured and simulated data were designated to demonstrate the effectiveness of the proposed model. By analyzing the results, conclusion was conducted that short headway can reduce passengers’ waiting time at bus stop. Meanwhile, the theoretical analysis explained the inefficiency of bus stops with more than three berths from the perspective of passengers’ additional walking time. Additional walking time will increase in a large scale when the number of berths at a bus stop exceedsthe threshold of three.

  13. Bar piezoelectric ceramic transformers.

    Science.gov (United States)

    Erhart, Jiří; Pulpan, Půlpán; Rusin, Luboš

    2013-07-01

    Bar-shaped piezoelectric ceramic transformers (PTs) working in the longitudinal vibration mode (k31 mode) were studied. Two types of the transformer were designed--one with the electrode divided into two segments of different length, and one with the electrodes divided into three symmetrical segments. Parameters of studied transformers such as efficiency, transformation ratio, and input and output impedances were measured. An analytical model was developed for PT parameter calculation for both two- and three-segment PTs. Neither type of bar PT exhibited very high efficiency (maximum 72% for three-segment PT design) at a relatively high transformation ratio (it is 4 for two-segment PT and 2 for three-segment PT at the fundamental resonance mode). The optimum resistive loads were 20 and 10 kΩ for two- and three-segment PT designs for the fundamental resonance, respectively, and about one order of magnitude smaller for the higher overtone (i.e., 2 kΩ and 500 Ω, respectively). The no-load transformation ratio was less than 27 (maximum for two-segment electrode PT design). The optimum input electrode aspect ratios (0.48 for three-segment PT and 0.63 for two-segment PT) were calculated numerically under no-load conditions.

  14. Aberrant Splicing in Cancer: Mediators of Malignant Progression through an Imperfect Splice Program Shift.

    Science.gov (United States)

    Luz, Felipe Andrés Cordero; Brígido, Paula Cristina; Moraes, Alberto Silva; Silva, Marcelo José Barbosa

    2017-01-01

    Although the efforts to understand the genetic basis of cancer allowed advances in diagnosis and therapy, little is known about other molecular bases. Splicing is a key event in gene expression, controlling the excision of introns decoded inside genes and being responsible for 80% of the proteome amplification through events of alternative splicing. Growing data from the last decade point to deregulation of splicing events as crucial in carcinogenesis and tumor progression. Several alterations in splicing events were observed in cancer, caused by either missexpression of or detrimental mutations in some splicing factors, and appear to be critical in carcinogenesis and key events during tumor progression. Notwithstanding, it is difficult to determine whether it is a cause or consequence of cancer and/or tumorigenesis. Most reviews focus on the generated isoforms of deregulated splicing pattern, while others mainly summarize deregulated splicing factors observed in cancer. In this review, events associated with carcinogenesis and tumor progression mainly, and epithelial-to-mesenchymal transition, which is also implicated in alternative splicing regulation, will be progressively discussed in the light of a new perspective, suggesting that splicing deregulation mediates cell reprogramming in tumor progression by an imperfect shift of the splice program.

  15. SAW: a method to identify splicing events from RNA-Seq data based on splicing fingerprints.

    Directory of Open Access Journals (Sweden)

    Kang Ning

    Full Text Available Splicing event identification is one of the most important issues in the comprehensive analysis of transcription profile. Recent development of next-generation sequencing technology has generated an extensive profile of alternative splicing. However, while many of these splicing events are between exons that are relatively close on genome sequences, reads generated by RNA-Seq are not limited to alternative splicing between close exons but occur in virtually all splicing events. In this work, a novel method, SAW, was proposed for the identification of all splicing events based on short reads from RNA-Seq. It was observed that short reads not in known gene models are actually absent words from known gene sequences. An efficient method to filter and cluster these short reads by fingerprint fragments of splicing events without aligning short reads to genome sequences was developed. Additionally, the possible splicing sites were also determined without alignment against genome sequences. A consensus sequence was then generated for each short read cluster, which was then aligned to the genome sequences. Results demonstrated that this method could identify more than 90% of the known splicing events with a very low false discovery rate, as well as accurately identify, a number of novel splicing events between distant exons.

  16. Compensatory relationship between splice sites and exonic splicing signals depending on the length of vertebrate introns

    Directory of Open Access Journals (Sweden)

    Rogozin Igor B

    2006-12-01

    Full Text Available Abstract Background The signals that determine the specificity and efficiency of splicing are multiple and complex, and are not fully understood. Among other factors, the relative contributions of different mechanisms appear to depend on intron size inasmuch as long introns might hinder the activity of the spliceosome through interference with the proper positioning of the intron-exon junctions. Indeed, it has been shown that the information content of splice sites positively correlates with intron length in the nematode, Drosophila, and fungi. We explored the connections between the length of vertebrate introns, the strength of splice sites, exonic splicing signals, and evolution of flanking exons. Results A compensatory relationship is shown to exist between different types of signals, namely, the splice sites and the exonic splicing enhancers (ESEs. In the range of relatively short introns (approximately, Conclusion Several weak but statistically significant correlations were observed between vertebrate intron length, splice site strength, and potential exonic splicing signals. Taken together, these findings attest to a compensatory relationship between splice sites and exonic splicing signals, depending on intron length.

  17. Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.

    Science.gov (United States)

    Aroian, R V; Levy, A D; Koga, M; Ohshima, Y; Kramer, J M; Sternberg, P W

    1993-01-01

    The dinucleotide AG, found at the 3' end of virtually all eukaryotic pre-mRNA introns, is thought to be essential for splicing. Reduction-of-function mutations in two Caenorhabditis elegans genes, the receptor tyrosine kinase gene let-23 and the collagen gene dpy-10, both alter the AG at the end of a short (ca. 50-nucleotide) intron to AA. The in vivo effects of these mutations were studied by sequencing polymerase chain reaction-amplified reverse-transcribed RNA isolated from the two mutants. As expected, we find transcripts that splice to a cryptic AG, skip an exon, and retain an unspliced intron. However, we also find significant levels of splicing at the mutated 3' splice site (AA) and at nearby non-AG dinucleotides. Our results indicate that for short C. elegans introns an AG is not required for splicing at either the correct 3' splice site or incorrect sites. Analysis of a splice site mutant involving a longer, 316-nucleotide C. elegans intron indicates that an AG is also not required there for splicing. We hypothesize that elements besides the invariant AG, e.g., an A-U-rich region, a UUUC motif, and/or a potential branch point sequence, are directing the selection of the 3' splice site and that in wild-type genes these elements cooperate so that proper splicing occurs. Images PMID:8417357

  18. The decays \\bar{B}->\\bar{K}D and \\bar{B}-> \\bar{K}\\bar{D} and final state interactions

    CERN Document Server

    Fayyazuddin, A

    2001-01-01

    The decays \\bar{B}-> \\bar{K}D and \\bar{B}-> \\bar{K}\\bar{D} taking into account final state interactions are discussed. These decays are described by four strong phases \\delta_0,\\delta_1,\\bar{\\delta}_0,\\bar{\\delta}_1 (subscripts 0 and 1 refers to I=0 and I=1 final states), one weak phase \\gamma and four real amplitudes. It is argued that strong interaction dynamics implies \\bar{\\delta}_1=0,\\delta_0=-\\delta_1. Rescattering has significant effects on weak amplitudes. Taking into account, rescattering, we find that direct CP--violating asymmetry in these decays may lie in the range \\mp 0.023\\sin \\gamma \\leq \\QTR{cal}{A}_{1,2}\\leq \\mp 0.086\\sin \\gamma.

  19. Extracting potential bus lines of Customized City Bus Service based on public transport big data

    Science.gov (United States)

    Ren, Yibin; Chen, Ge; Han, Yong; Zheng, Huangcheng

    2016-11-01

    Customized City Bus Service (CCBS) can reduce the traffic congestion and environmental pollution that caused by the increasing in private cars, effectively. This study aims to extract the potential bus lines and each line's passenger density of CCBS by mining the public transport big data. The datasets used in this study are mainly Smart Card Data (SCD) and bus GPS data of Qingdao, China, from October 11th and November 7th 2015. Firstly, we compute the temporal-origin-destination (TOD) of passengers by mining SCD and bus GPS data. Compared with the traditional OD, TOD not only has the spatial location, but also contains the trip's boarding time. Secondly, based on the traditional DBSCAN algorithm, we put forwards an algorithm, named TOD-DBSCAN, combined with the spatial-temporal features of TOD.TOD-DBSCAN is used to cluster the TOD trajectories in peak hours of all working days. Then, we define two variables P and N to describe the possibility and passenger destiny of a potential CCBS line. P is the probability of the CCBS line. And N represents the potential passenger destiny of the line. Lastly, we visualize the potential CCBS lines extracted by our procedure on the map and analyse relationship between potential CCBS lines and the urban spatial structure.

  20. Effect of stoppage time on motion of a bus through a sequence of signals

    Science.gov (United States)

    Nagatani, Takashi

    2017-01-01

    We study the dynamic motion of a bus moving through the series of traffic signals where the bus stops at bus stops during a time. The dynamic state of the bus depends highly on both stoppage time at the bus stop and cycle time of the signal. It is found that the bus motion has two kinds of dynamic states: the one is the normal state and the other is the offset state. In the normal state, the bus stops normally at both bus stops and signals. In the offset state, the bus passes timely through the signal sometimes and the stoppage time at the bus stop is offset against the stopping time at the signal. If the bus speed has the same value as the car speed, the travel time of the bus is consistent with that of the car in the offset state. The region map (phase diagram) is shown for two kinds of dynamic states: the normal and offset states.

  1. Simulation Model of Bus Rapid Transit

    Directory of Open Access Journals (Sweden)

    Gunawan Fergyanto E.

    2014-03-01

    Full Text Available Bus rapid transit system is modern solution for mass transportation system. The system, in comparison to the rail-based transportation system, is significantly cheaper and requires shorter development time, but lower performance. The BRT system performance strongly depends on variables related to station design and infrastructure. A numerical model offers an effective and efficient means to evaluate the system performance. This article offers a detailed numerical model on the basis of the discrete-event approach and demonstrates its application.

  2. Oracle Service Bus 11g Development Cookbook

    CERN Document Server

    Schmutz, Guido; van Zoggel, Jan

    2012-01-01

    This cookbook is full of immediately useable recipes showing you how to develop service and message-oriented (integration) applications on the Oracle Service Bus. In addition to its cookbook style, which ensures the solutions are presented in a clear step-by-step manner, the explanations go into great detail, which makes it good learning material for everyone who has experience in OSB and wants to improve. Most of the recipes are designed in such a way that each recipe is presented as a separate, standalone entity and reading of prior recipes is not required. The finished solution of each reci

  3. Redesign of Transjakarta Bus Driver's Cabin

    Science.gov (United States)

    Mardi Safitri, Dian; Azmi, Nora; Singh, Gurbinder; Astuti, Pudji

    2016-02-01

    Ergonomic risk at work stations with type Seated Work Control was one of the problems faced by Transjakarta bus driver. Currently “Trisakti” type bus, one type of bus that is used by Transjakarta in corridor 9, serving route Pinang Ranti - Pluit, gained many complaints from drivers. From the results of Nordic Body Map questionnaires given to 30 drivers, it was known that drivers feel pain in the neck, arms, hips, and buttocks. Allegedly this was due to the seat position and the button/panel bus has a considerable distance range (1 meter) to be achieved by drivers. In addition, preliminary results of the questionnaire using Workstation Checklist identified their complaints about uncomfortable cushion, driver's seat backrest, and the exact position of the AC is above the driver head. To reduce the risk level of ergonomics, then did research to design the cabin by using a generic approach to designing products. The risk analysis driver posture before the design was done by using Rapid Upper Limb Assessment (RULA), Rapid Entire Body Assessment (REBA), and Quick Exposure Checklist (QEC), while the calculation of the moment the body is done by using software Mannequin Pro V10.2. Furthermore, the design of generic products was done through the stages: need metric-matrix, house of quality, anthropometric data collection, classification tree concept, concept screening, scoring concept, design and manufacture of products in the form of two-dimensional. While the design after design risk analysis driver posture was done by using RULA, REBA, and calculation of moments body as well as the design visualized using software 3DMax. From the results of analysis before the draft design improvements cabin RULA obtained scores of 6, REBA 9, and the result amounted to 57.38% QEC and moment forces on the back is 247.3 LbF.inch and on the right hip is 72.9 LbF.in. While the results of the proposed improvements cabin design RULA obtained scores of 3, REBA 4, and the moment of force on

  4. It's Time to Move for School Bus

    Institute of Scientific and Technical Information of China (English)

    Amy Hu

    2011-01-01

    @@ The 1st China School Buses Development Seminar &.International School Buses Expo will be held from February 15 to 18, 2012 in the National Convention Center in Beijing, according to the National Office of Education on the Traffic Safety of Primary and Secondary School Students.It aims to publicize the Chinese standards for school bus safety, improve the development, production and operation of school buses in China and the rest of the world, promote the application new technologies on school buses, and call on people to care about and support the development of school buses.

  5. Depolarization-mediated regulation of alternative splicing

    Directory of Open Access Journals (Sweden)

    Alok eSharma

    2011-12-01

    Full Text Available Alternative splicing in eukaryotes plays an important role in regulating gene expression by selectively including alternative exons. A wealth of information has been accumulated that explains how alternative exons are selected in a developmental stage- or tissue-specific fashion. However, our knowledge of how cells respond to environmental changes to alter alternative splicing is very limited. For example, although a number of alternative exons have been shown to be regulated by calcium level alterations, the underlying mechanisms are not well understood. As calcium signaling in neurons plays a crucial role in essential neuronal functions such as learning and memory formation, it is important to understand how this process is regulated at every level in gene expression. The significance of the dynamic control of alternative splicing in response to changes of calcium levels has been largely unappreciated. In this communication, we will summarize the recent advances in calcium signaling-mediated alternative splicing that have provided some insights into the important regulatory mechanisms. In addition to describing the cis-acting RNA elements on the pre-mRNA molecules that respond to changes of intracellular calcium levels, we will summarize how splicing regulators change and affect alternative splicing in this process. We will also discuss a novel mode of calcium-mediated splicing regulation at the level of chromatin structure and transcription.

  6. Assisted transcriptome reconstruction and splicing orthology

    Directory of Open Access Journals (Sweden)

    Samuel Blanquart

    2016-11-01

    Full Text Available Abstract Background Transcriptome reconstruction, defined as the identification of all protein isoforms that may be expressed by a gene, is a notably difficult computational task. With real data, the best methods based on RNA-seq data identify barely 21 % of the expressed transcripts. While waiting for algorithms and sequencing techniques to improve — as has been strongly suggested in the literature — it is important to evaluate assisted transcriptome prediction; this is the question of how alternative transcription in one species performs as a predictor of protein isoforms in another relatively close species. Most evidence-based gene predictors use transcripts from other species to annotate a genome, but the predictive power of procedures that use exclusively transcripts from external species has never been quantified. The cornerstone of such an evaluation is the correct identification of pairs of transcripts with the same splicing patterns, called splicing orthologs. Results We propose a rigorous procedural definition of splicing orthologs, based on the identification of all ortholog pairs of splicing sites in the nucleotide sequences, and alignments at the protein level. Using our definition, we compared 24 382 human transcripts and 17 909 mouse transcripts from the highly curated CCDS database, and identified 11 122 splicing orthologs. In prediction mode, we show that human transcripts can be used to infer over 62 % of mouse protein isoforms. When restricting the predictions to transcripts known eight years ago, the percentage grows to 74 %. Using CCDS timestamped releases, we also analyze the evolution of the number of splicing orthologs over the last decade. Conclusions Alternative splicing is now recognized to play a major role in the protein diversity of eukaryotic organisms, but definitions of spliced isoform orthologs are still approximate. Here we propose a definition adapted to the subtle variations of conserved alternative

  7. Using the Mil. Std 1553B data bus in future spacecraft onboard applications

    Science.gov (United States)

    Plummer, Chris; Bordes, Yves

    2002-07-01

    This paper discusses the use of the Mil. Std 1553B data bus as the principal onboard data handling bus for future spacecraft applications. The paper takes a pragmatic approach by: Identifying the characteristics of the onboard bus traffic and its characteristics; Looking at future trends in onboard bus traffic; Describing the characteristics of the Mil. Std 1553B data bus; Proposing techniques that can be used on the Mil. Std 1553B data bus in future spacecraft application.

  8. Regulation of Alternative Splicing in Vivo by Overexpression of Antagonistic Splicing Factors

    Science.gov (United States)

    Caceres, Javier F.; Stamm, Stefan; Helfman, David M.; Krainer, Adrian R.

    1994-09-01

    The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.

  9. Review of bus drivers' occupational stress and stress prevention

    NARCIS (Netherlands)

    Kompier, M.A.J.; Di Martino, V.

    1995-01-01

    Although heterogeneous in methodology and content, 32 studies from 13 countries on bus drivers' work and health are similar in their conclusions. Bus-driving–characterized by high demands, low control and low support – can be regarded as a classic example of high-strain occupation, with high risks o

  10. Network Analysis of Urban Traffic with Big Bus Data

    CERN Document Server

    Zhao, Kai

    2016-01-01

    Urban traffic analysis is crucial for traffic forecasting systems, urban planning and, more recently, various mobile and network applications. In this paper, we analyse urban traffic with network and statistical methods. Our analysis is based on one big bus dataset containing 45 million bus arrival samples in Helsinki. We mainly address following questions: 1. How can we identify the areas that cause most of the traffic in the city? 2. Why there is a urban traffic? Is bus traffic a key cause of the urban traffic? 3. How can we improve the urban traffic systems? To answer these questions, first, the betweenness is used to identify the most import areas that cause most traffics. Second, we find that bus traffic is not an important cause of urban traffic using statistical methods. We differentiate the urban traffic and the bus traffic in a city. We use bus delay as an identification of the urban traffic, and the number of bus as an identification of the bus traffic. Third, we give our solutions on how to improve...

  11. 32 CFR 935.138 - Motor bus operation.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Motor bus operation. 935.138 Section 935.138 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND CODE Motor Vehicle Code § 935.138 Motor bus operation. Each person operating a...

  12. Network Extender for MIL-STD-1553 Bus

    Science.gov (United States)

    Marcus, Julius; Hanson, T. David

    2003-01-01

    An extender system for MIL-STD-1553 buses transparently couples bus components at multiple developer sites. The bus network extender is a relatively inexpensive system that minimizes the time and cost of integration of avionic systems by providing a convenient mechanism for early testing without the need to transport the usual test equipment and personnel to an integration facility. This bus network extender can thus alleviate overloading of the test facility while enabling the detection of interface problems that can occur during the integration of avionic systems. With this bus extender in place, developers can correct and adjust their own hardware and software before products leave a development site. Currently resident at Johnson Space Center, the bus network extender is used to test the functionality of equipment that, although remotely located, is connected through a MILSTD- 1553 bus. Inasmuch as the standard bus protocol for avionic equipment is that of MIL-STD-1553, companies that supply MIL-STD-1553-compliant equipment to government or industry and that need long-distance communication support might benefit from this network bus extender

  13. A High Efficiency DC Bus Regulator / RPC for Spacecraft Applications

    Science.gov (United States)

    Birchenough, Arthur G.

    2004-02-01

    DC bus voltage regulation may be required in future high powered spacecraft due to the length of the busses or because they are not generated at precise voltage levels. In these cases the regulation range is often only a few percent increase or decrease, but conventional DC voltage regulators switch all the power passing through them, and this level of power switched determines the size and losses in the regulator. A recently developed concept uses a low power DC-DC converter in series with the bus to raise or lower the bus voltage over a small range. This partial power processing technique combines the small size and power losses of the low power converter with the ability to regulate, (over a small range) a high power bus. The Series Connected Buck Boost Regulator (SCBBR) described herein provides bus regulation with an efficiency of 98%. The circuit also provides bus switching and overcurrent limiting functions of a Remote Power Controller (RPC). This paper describes the circuit design and performance of a breadboard SCBBR configured as a bus voltage regulator providing +/- 40% voltage regulation range, bus switching, and overload limiting.

  14. A decentralized software bus based on IP multicas ting

    Science.gov (United States)

    Callahan, John R.; Montgomery, Todd

    1995-01-01

    We describe decentralized reconfigurable implementation of a conference management system based on the low-level Internet Protocol (IP) multicasting protocol. IP multicasting allows low-cost, world-wide, two-way transmission of data between large numbers of conferencing participants through the Multicasting Backbone (MBone). Each conference is structured as a software bus -- a messaging system that provides a run-time interconnection model that acts as a separate agent (i.e., the bus) for routing, queuing, and delivering messages between distributed programs. Unlike the client-server interconnection model, the software bus model provides a level of indirection that enhances the flexibility and reconfigurability of a distributed system. Current software bus implementations like POLYLITH, however, rely on a centralized bus process and point-to-point protocols (i.e., TCP/IP) to route, queue, and deliver messages. We implement a software bus called the MULTIBUS that relies on a separate process only for routing and uses a reliable IP multicasting protocol for delivery of messages. The use of multicasting means that interconnections are independent of IP machine addresses. This approach allows reconfiguration of bus participants during system execution without notifying other participants of new IP addresses. The use of IP multicasting also permits an economy of scale in the number of participants. We describe the MULITIBUS protocol elements and show how our implementation performs better than centralized bus implementations.

  15. Djungle power. A more remote AC bus

    Energy Technology Data Exchange (ETDEWEB)

    Boehnke, Heinz W. [Technosol, Jork (Germany)

    2010-07-01

    The Dzanga-Sangha Reserve in the Central African Republic is probably one of the world's most remote protected rainforest areas, much to the benefit of the rare species of lowland gorilla, forest elephant and local pygmy population Ba'Aka. The park is co-managed by the ''WWF-World Wide Fund for Nature'' with sponsoring from the German Regenwald-Stiftung. In 2008 wwf decided to switch from the old, failing generator supply to a solar hybrid mini-grid, along with stringent measures of energy conservation and management. After a careful energy audit Technosol designed an AC-bus system with a 22 kWp PV generator which should overcome the dependency on the old generators and their fuel demands. For 15 buildings, an entirely new distribution network was installed, state of the art energy saving devices introduced and an energy metering system deviced. The installation is operating since August 2009. In such remote locations, the reliability of the AC-bus over other generator-based solutions will be demonstrated and with the support of international organisations like the wwf the practical application of solar hybrid supply becomes a landmark of sustainable energy concepts. (orig.)

  16. Anti-tumor activity of splice-switching oligonucleotides

    OpenAIRE

    Bauman, John A; Li, Shyh-Dar; Yang, Angela; Huang, Leaf; Kole, Ryszard

    2010-01-01

    Alternative splicing has emerged as an important target for molecular therapies. Splice-switching oligonucleotides (SSOs) modulate alternative splicing by hybridizing to pre-mRNA sequences involved in splicing and blocking access to the transcript by splicing factors. Recently, the efficacy of SSOs has been established in various animal disease models; however, the application of SSOs against cancer targets has been hindered by poor in vivo delivery of antisense therapeutics to tumor cells. T...

  17. Mercer County off-peak fare-free bus demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Dommasch, I.N.; Hollinger, R.L.

    1979-07-01

    A system-wide, off-peak fare-free bus demonstration project is being conducted in Mercer County, New Jersey to study effects of fare-free bus operation. Data collected for 2 1/2 months of operation are presented. Since implementation of the fare-free service, Mercer Metro off-peak ridership has increased approximately 45 to 50%. This equates to an overall system-wide ridership increase of about 10 to 15%. One effect of the fare-free operation has been to increase bus run times, caused in part by accompanying increases in the occurrence and length of bus delays in the downtown area. The percentage of filled bus seats has changed from about 10% before the demonstration, to over 50% after the off-peak fares were eliminated.

  18. An Overview of Staged-bus Operation in Cities

    Directory of Open Access Journals (Sweden)

    Mohammad Hesam Hafezi

    2014-02-01

    Full Text Available In developing countries, demand for passenger travel is higher, due to economic growth. Government policy encourages people to use public transportation instead of their private carsto reduce of traffic congestion and air pollution. In the city areas, bus networks are the major part of the public transportation system because they are easily accessible and cheaper compared to other types of public transportation. In this study revelation of details of bus operation included different agents is studied. Bus operations, during provision of their service, have interaction with three main agents: bus agent, passenger agent and traffic agent. Study of the influence of each agent in bus operation is presented in this study, using the macroscopic way.

  19. Research on the Coordinated Design of Bus and Taxi Station

    Directory of Open Access Journals (Sweden)

    Jiangfeng Xi

    2015-01-01

    Full Text Available Rises in the number of transit buses, bus routes, and overall traffic volume in China’s cities, coupled with interference from other transport modes, such as taxis loading and unloading passengers nearby, have led to increasing traffic delays at bus stops, which is considered one of the factors degrading service levels and traffic operations on urban roadways. This paper studies traffic characteristics at bus stops, investigates variations in delay from different types or designs of bus stops, and analyzes the impact of it on traffic capacity, the purpose of which is to propose a solution to predicting the feasibility of an integrated design of bus stops and taxi stands with the help of mathematical models and based on the objectives of optimal traffic operations and passenger transfer.

  20. Method and systems for a radiation tolerant bus interface circuit

    Science.gov (United States)

    Kinstler, Gary A. (Inventor)

    2007-01-01

    A bus management tool that allows communication to be maintained between a group of nodes operatively connected on two busses in the presence of radiation by transmitting periodically a first message from one to another of the nodes on one of the busses, determining whether the first message was received by the other of the nodes on the first bus, and when it is determined that the first message was not received by the other of the nodes, transmitting a recovery command to the other of the nodes on a second of the of busses. Methods, systems, and articles of manufacture consistent with the present invention also provide for a bus recovery tool on the other node that re-initializes a bus interface circuit operatively connecting the other node to the first bus in response to the recovery command.

  1. MATHEMATIC MODEL FOR SITY BUS SCHEDULING IN YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Sahid Sahid

    2016-05-01

    Full Text Available Various methods can be used to construct a mathematical model of the transportation problems. One model that can be used is a linear model. Several studies have used a linear model to get the schedule and the optimal route of bus trips. This research will build a mathematical model of a city bus transportation problems in DIY using linear models. Linear model is built to get the condition density city bus passengers on shifts respectively that morning, noon, and evening. After finding a suitable model, applied to the bus passengers data in Yogyakarta. From these results it can be seen the optimum conditions in terms of density, because the condition of the city bus at this time that quiet enthusiasts. Besides, the optimum density at each shift in the morning is 11 passengers, 10 passengers during the day, and evening 9 passengers.   Keywords: transportation problems, the linear model, the optimal route, density

  2. Faster exon assembly by sparse spliced alignment

    CERN Document Server

    Tiskin, Alexander

    2007-01-01

    Assembling a gene from candidate exons is an important problem in computational biology. Among the most successful approaches to this problem is \\emph{spliced alignment}, proposed by Gelfand et al., which scores different candidate exon chains within a DNA sequence of length $m$ by comparing them to a known related gene sequence of length n, $m = \\Theta(n)$. Gelfand et al.\\ gave an algorithm for spliced alignment running in time O(n^3). Kent et al.\\ considered sparse spliced alignment, where the number of candidate exons is O(n), and proposed an algorithm for this problem running in time O(n^{2.5}). We improve on this result, by proposing an algorithm for sparse spliced alignment running in time O(n^{2.25}). Our approach is based on a new framework of \\emph{quasi-local string comparison}.

  3. Protein splicing and its evolution in eukaryotes

    Directory of Open Access Journals (Sweden)

    Starokadomskyy P. L.

    2010-02-01

    Full Text Available Inteins, or protein introns, are parts of protein sequences that are post-translationally excised, their flanking regions (exteins being spliced together. This process was called protein splicing. Originally inteins were found in prokaryotic or unicellular eukaryotic organisms. But the general principles of post-translation protein rearrangement are evolving yielding different post-translation modification of proteins in multicellular organisms. For clarity, these non-intein mediated events call either protein rearrangements or protein editing. The most intriguing example of protein editing is proteasome-mediated splicing of antigens in vertebrates that may play important role in antigen presentation. Other examples of protein rearrangements are maturation of Hg-proteins (critical receptors in embryogenesis as well as maturation of several metabolic enzymes. Despite a lack of experimental data we try to analyze some intriguing examples of protein splicing evolution.

  4. HOLLYWOOD: a comparative relational database of alternative splicing.

    Science.gov (United States)

    Holste, Dirk; Huo, George; Tung, Vivian; Burge, Christopher B

    2006-01-01

    RNA splicing is an essential step in gene expression, and is often variable, giving rise to multiple alternatively spliced mRNA and protein isoforms from a single gene locus. The design of effective databases to support experimental and computational investigations of alternative splicing (AS) is a significant challenge. In an effort to integrate accurate exon and splice site annotation with current knowledge about splicing regulatory elements and predicted AS events, and to link information about the splicing of orthologous genes in different species, we have developed the Hollywood system. This database was built upon genomic annotation of splicing patterns of known genes derived from spliced alignment of complementary DNAs (cDNAs) and expressed sequence tags, and links features such as splice site sequence and strength, exonic splicing enhancers and silencers, conserved and non-conserved patterns of splicing, and cDNA library information for inferred alternative exons. Hollywood was implemented as a relational database and currently contains comprehensive information for human and mouse. It is accompanied by a web query tool that allows searches for sets of exons with specific splicing characteristics or splicing regulatory element composition, or gives a graphical or sequence-level summary of splicing patterns for a specific gene. A streamlined graphical representation of gene splicing patterns is provided, and these patterns can alternatively be layered onto existing information in the UCSC Genome Browser. The database is accessible at http://hollywood.mit.edu.

  5. Bars in Cuspy Dark Halos

    CERN Document Server

    Dubinski, John; Shlosman, Isaac

    2008-01-01

    We examine the bar instability in models with an exponential disk and a cuspy NFW-like dark matter (DM) halo inspired by cosmological simulations. Bar evolution is studied as a function of numerical resolution in a sequence of models spanning 10K to 100M DM particles - including a multi-mass model with an effective resolution of 10G. The goal is to find convergence in dynamical behaviour. We characterize the bar growth, the buckling instability, pattern speed decay through resonant transfer of angular momentum, and possible destruction of the DM halo cusp. Overall, most characteristics converge in behaviour in detail for halos containing more than 10M particles. Notably, the formation of the bar does not destroy the density cusp in this case. These higher resolution simulations clearly illustrate the importance of discrete resonances in transporting angular momentum from the bar to the halo.

  6. Alcoholism and Alternative Splicing of Candidate Genes

    OpenAIRE

    Toshikazu Sasabe; Shoichi Ishiura

    2010-01-01

    Gene expression studies have shown that expression patterns of several genes have changed during the development of alcoholism. Gene expression is regulated not only at the level of transcription but also through alternative splicing of pre-mRNA. In this review, we discuss some of the evidence suggesting that alternative splicing of candidate genes such as DRD2 (encoding dopamine D2 receptor) may form the basis of the mechanisms underlying the pathophysiology of alcoholism. These reports sugg...

  7. Galaxy Zoo: CANDELS Barred Disks and Bar Fractions

    CERN Document Server

    Simmons, B D; Lintott, Chris; Masters, Karen L; Willett, Kyle W; Keel, William C; Smethurst, R J; Cheung, Edmond; Nichol, Robert C; Schawinski, Kevin; Rutkowski, Michael; Kartaltepe, Jeyhan S; Bell, Eric F; Casteels, Kevin R V; Conselice, Christopher J; Almaini, Omar; Ferguson, Henry C; Fortson, Lucy; Hartley, William; Kocevski, Dale; Koekemoer, Anton M; McIntosh, Daniel H; Mortlock, Alice; Newman, Jeffrey A; Ownsworth, Jamie; Bamford, Steven; Dahlen, Tomas; Faber, Sandra M; Finkelstein, Steven L; Fontana, Adriano; Galametz, Audrey; Grogin, N A; Grutzbauch, Ruth; Guo, Yicheng; Haussler, Boris; Jek, Kian J; Kaviraj, Sugata; Lucas, Ray A; Peth, Michael; Salvato, Mara; Wiklind, Tommy; Wuyts, Stijn

    2014-01-01

    The formation of bars in disk galaxies is a tracer of the dynamical maturity of the population. Previous studies have found that the incidence of bars in disks decreases from the local Universe to z ~ 1, and by z > 1 simulations predict that bar features in dynamically mature disks should be extremely rare. Here we report the discovery of strong barred structures in massive disk galaxies at z ~ 1.5 in deep rest-frame optical images from CANDELS. From within a sample of 876 disk galaxies identified by visual classification in Galaxy Zoo, we identify 123 barred galaxies. Selecting a sub-sample within the same region of the evolving galaxy luminosity function (brighter than L*), we find that the bar fraction across the redshift range 0.5< z < 2 (f_bar = 10.7 +6.3 -3.5% after correcting for incompleteness) does not significantly evolve. We discuss the implications of this discovery in the context of existing simulations and our current understanding of the way disk galaxies have evolved over the last 11 bil...

  8. Targeting RNA-splicing for SMA treatment.

    Science.gov (United States)

    Zhou, Jianhua; Zheng, Xuexiu; Shen, Haihong

    2012-03-01

    The central dogma of DNA-RNA-protein was established more than 40 years ago. However, important biological processes have been identified since the central dogma was developed. For example, methylation is important in the regulation of transcription. In contrast, proteins, are more complex due to modifications such as phosphorylation, glycosylation, ubiquitination, or cleavage. RNA is the mediator between DNA and protein, but it can also be modulated at several levels. Among the most profound discoveries of RNA regulation is RNA splicing. It has been estimated that 80% of pre-mRNA undergo alternative splicing, which exponentially increases biological information flow in cellular processes. However, an increased number of regulated steps inevitably accompanies an increased number of errors. Abnormal splicing is often found in cells, resulting in protein dysfunction that causes disease. Splicing of the survival motor neuron (SMN) gene has been extensively studied during the last two decades. Accumulating knowledge on SMN splicing has led to speculation and search for spinal muscular atrophy (SMA) treatment by stimulating the inclusion of exon 7 into SMN mRNA. This mini-review summaries the latest progress on SMN splicing research as a potential treatment for SMA disease.

  9. Work fatigue in urban bus drivers

    Directory of Open Access Journals (Sweden)

    Teresa Makowiec-Dąbrowska

    2015-10-01

    Full Text Available Background: Bus drivers are a special group of professional drivers who are at a very high risk of fatigue. The aim of the study was to examine whether the driver’s subjective assessment of fatigue allows for the determination of its level and identification of its causes. Material and Methods: The study group comprised 45 randomly selected bus drivers (mean age – 43.7±7.9 years, period of employment as drivers – 14.7±8.6 years. Examinations were performed in all subjects four times – before and after work on the “easy” route (outside the city center, small traffic intensity and before and after work on the “difficult” route (city center, heavy traffic. The fatigue test questionnaire, based on the list of symptoms of fatigue prepared by the Japan Research Committee of Fatigue, was used in the study. Results: The rating of fatigue after the work was significantly higher than that before the work. The profile of fatigue after work was not influenced by the type of route, but the assessment of most symptoms of fatigue reached a higher level after the “difficult” routes and the differences were statistically significant for 7 symptoms. Only the ratings of leg fatigue, feeling of heaviness, and the necessity to squint eyes and gaze with effort reached the higher levels after driving the “easy” routes. It has been found that the level of fatigue was significantly correlated with the job characteristics (driving time, the length of the route, number of stops, etc. and with the abundance of food ingested and type of beverage (coffee vs. others drunk prior to driving. Conclusions: The questionnaire used in our study to assess the subjective feeling of fatigue has proved to be a sensitive and useful tool for indicating the level and causes of fatigue. The relationship between the symptoms of fatigue and the characteristics of job and lifestyle shows that actions must be taken by both the employers and employees to prevent fatigue

  10. The wheels on the bus go "buy buy buy": school bus advertising laws.

    Science.gov (United States)

    Pomeranz, Jennifer L

    2012-09-01

    School buses, a practical necessity for millions of children, are at the center of new efforts to raise revenue. School bus advertising laws bring public health and commercialization concerns to the school setting. In doing so, they potentially expose school districts to First Amendment lawsuits. I examined various school bus advertising bills and laws. I reviewed First Amendment "forum analysis" as applied in the transit and school settings to clarify how this legal test may affect school districts subject to such laws. I have made recommendations for school districts to enact appropriate policies to ensure that such advertising does not undermine public health and to enable the districts to maintain control over their property.

  11. Repairing and strengthening a fractured Hader bar.

    Science.gov (United States)

    Herrero, D B

    1997-01-01

    Stresses from occlusion and metal fatigue over time can cause fracture of overdenture retention bars. Often failure of the bar necessitates the removal and remake of the bar. This may damage the abutment, especially if there are dowel posts involved. This article describes a method for reinforcing the existing bar without having to remove the bar or significantly alter the overdenture. The fractured bar can be prepared in the mouth to receive a reinforcing superstructure that will be cemented.

  12. Strategic nonviolent conflict: The Montgomery Bus Boycott

    Directory of Open Access Journals (Sweden)

    Fatih Balcı

    2011-07-01

    Full Text Available Nonviolent action emerges under the conditions of recognized an unacceptable political oppression and injustice. The people, who find the situation unacceptable, develop three ways of behavior. First, they exit the situation. Second, they engage in everyday forms of resistance. Third, they participate in political action which has two aspects: institutional and non-institutional. Institutional political action comprises of the regular political activities such as voting, taking part in legislative process, and joining political parties. Non-institutional political action, on the contrary, arises as either violent action or nonviolent action. This paper examines the emergence, progress, and spread of the Montgomery Bus Boycott as one of the leading examples of the nonviolent struggles in the last century.

  13. Strategic nonviolent conflict: The Montgomery Bus Boycott

    Directory of Open Access Journals (Sweden)

    Hüdaverdi Balcı

    2011-07-01

    Full Text Available Nonviolent action emerges under the conditions of recognized an unacceptable political oppression and injustice. The people, who find the situation unacceptable, develop three ways of behavior. First, they exit the situation. Second, they engage in everyday forms of resistance. Third, they participate in political action which has two aspects: institutional and non-institutional. Institutional political action comprises of the regular political activities such as voting, taking part in legislative process, and joining political parties. Non-institutional political action, on the contrary, arises as either violent action or nonviolent action. This paper examines the emergence, progress, and spread of the Montgomery Bus Boycott as one of the leading examples of the nonviolent struggles in the last century

  14. Task Analysis Assessment on Intrastate Bus Traffic Controllers

    Science.gov (United States)

    Yen Bin, Teo; Azlis-Sani, Jalil; Nur Annuar Mohd Yunos, Muhammad; Ismail, S. M. Sabri S. M.; Tajedi, Noor Aqilah Ahmad

    2016-11-01

    Public transportation acts as social mobility and caters the daily needs of the society for passengers to travel from one place to another. This is true for a country like Malaysia where international trade has been growing significantly over the past few decades. Task analysis assessment was conducted with the consideration of cognitive ergonomic view towards problem related to human factors. Conducting research regarding the task analysis on bus traffic controllers had allowed a better understanding regarding the nature of work and the overall monitoring activities of the bus services. This paper served to study the task analysis assessment on intrastate bus traffic controllers and the objectives of this study include to conduct task analysis assessment on the bus traffic controllers. Task analysis assessment for the bus traffic controllers was developed via Hierarchical Task Analysis (HTA). There are a total of five subsidiary tasks on level one and only two were able to be further broken down in level two. Development of HTA allowed a better understanding regarding the work and this could further ease the evaluation of the tasks conducted by the bus traffic controllers. Thus, human error could be reduced for the safety of all passengers and increase the overall efficiency of the system. Besides, it could assist in improving the operation of the bus traffic controllers by modelling or synthesizing the existing tasks if necessary.

  15. Integrated optics bus access module for intramachine communication

    Science.gov (United States)

    Karioja, Pentti; Tammela, Simo K. T.; Hannula, Tapio

    1991-12-01

    The feasibility of a passive bidirectional fiber optic bus and the packaging considerations of a bus access module have been studied. The bus uses 110/125 micrometers HCS fiber and passive integrated optic couplers for bus access. The access couplers are asymmetric and were fabricated using a Ag-Na ion exchange process. The asymmetry of the coupler was 5 dB, the launch loss to the bus was 6 dB and the tap-off loss to the node was 11 dB. With the integrated optics coupler it is possible to connect 6 nodes to the bidirectional bus. It is also possible to realize a simple, easy-to-use, and reliable bus access module for intramachine communication. The integrated optics coupler, a LED chip, and a PIN-diode chip and transceiver electronics are packaged in an electrical connector with a two-fiber optical cable pigtail. Active and passive components are butt coupled to the coupler. The 0.5 dB alignment tolerances for the fiber pigtails, the LED, and the PIN-diode chips are +/- 5 micrometers .

  16. Scheduling Combination Optimization Research for Bus Lane Line

    Directory of Open Access Journals (Sweden)

    Yazao Yang

    2013-07-01

    Full Text Available Different scheduling forms can be adopted in bus lane system to meet passengers’ travel demand well and improve operational efficiency. Therefore, this paper researched the optimal headway and bus scheduling combination of a bus lane line. Bus scheduling combination is composed of a sequence of full-length, express bus and short-turn. We established a model to minimize passengers’ waiting cost and vehicles’ operation cost and to optimize headway and bus scheduling combination, under the constraint of the headway restriction for each stop, the minimum number of vehicle trips in one hour and the proportion of short-turn and express bus trips in total trips. The model was solved by improved genetic algorithm, and the optimal solution was obtained by repeating the operation of genetic algorithm L times. The results of numerical example show that the whole cost can be saved by 21.77% at most after optimization, which indicate the model and algorithm we presented are reasonable and practicable.

  17. A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection.

    Science.gov (United States)

    Hoffmann, Steve; Otto, Christian; Doose, Gero; Tanzer, Andrea; Langenberger, David; Christ, Sabina; Kunz, Manfred; Holdt, Lesca M; Teupser, Daniel; Hackermüller, Jörg; Stadler, Peter F

    2014-02-10

    Numerous high-throughput sequencing studies have focused on detecting conventionally spliced mRNAs in RNA-seq data. However, non-standard RNAs arising through gene fusion, circularization or trans-splicing are often neglected. We introduce a novel, unbiased algorithm to detect splice junctions from single-end cDNA sequences. In contrast to other methods, our approach accommodates multi-junction structures. Our method compares favorably with competing tools for conventionally spliced mRNAs and, with a gain of up to 40% of recall, systematically outperforms them on reads with multiple splits, trans-splicing and circular products. The algorithm is integrated into our mapping tool segemehl (http://www.bioinf.uni-leipzig.de/Software/segemehl/).

  18. RNA splicing. The human splicing code reveals new insights into the genetic determinants of disease.

    Science.gov (United States)

    Xiong, Hui Y; Alipanahi, Babak; Lee, Leo J; Bretschneider, Hannes; Merico, Daniele; Yuen, Ryan K C; Hua, Yimin; Gueroussov, Serge; Najafabadi, Hamed S; Hughes, Timothy R; Morris, Quaid; Barash, Yoseph; Krainer, Adrian R; Jojic, Nebojsa; Scherer, Stephen W; Blencowe, Benjamin J; Frey, Brendan J

    2015-01-01

    To facilitate precision medicine and whole-genome annotation, we developed a machine-learning technique that scores how strongly genetic variants affect RNA splicing, whose alteration contributes to many diseases. Analysis of more than 650,000 intronic and exonic variants revealed widespread patterns of mutation-driven aberrant splicing. Intronic disease mutations that are more than 30 nucleotides from any splice site alter splicing nine times as often as common variants, and missense exonic disease mutations that have the least impact on protein function are five times as likely as others to alter splicing. We detected tens of thousands of disease-causing mutations, including those involved in cancers and spinal muscular atrophy. Examination of intronic and exonic variants found using whole-genome sequencing of individuals with autism revealed misspliced genes with neurodevelopmental phenotypes. Our approach provides evidence for causal variants and should enable new discoveries in precision medicine.

  19. BRCA1 Exon 11, a CERES (Composite Regulatory Element of Splicing Element Involved in Splice Regulation

    Directory of Open Access Journals (Sweden)

    Claudia Tammaro

    2014-07-01

    Full Text Available Unclassified variants (UV of BRCA1 can affect normal pre-mRNA splicing. Here, we investigate the UV c.693G>A, a “silent” change in BRCA1 exon 11, which we have found induces aberrant splicing in patient carriers and in vitro. Using a minigene assay, we show that the UV c.693G>A has a strong effect on the splicing isoform ratio of BRCA1. Systematic site-directed mutagenesis of the area surrounding the nucleotide position c.693G>A induced variable changes in the level of exon 11 inclusion/exclusion in the mRNA, pointing to the presence of a complex regulatory element with overlapping enhancer and silencer functions. Accordingly, protein binding analysis in the region detected several splicing regulatory factors involved, including SRSF1, SRSF6 and SRSF9, suggesting that this sequence represents a composite regulatory element of splicing (CERES.

  20. Vitamin D and alternative splicing of RNA.

    Science.gov (United States)

    Zhou, Rui; Chun, Rene F; Lisse, Thomas S; Garcia, Alejandro J; Xu, Jianzhong; Adams, John S; Hewison, Martin

    2015-04-01

    The active form of vitamin D (1α,25-dihydroxyvitamin D, 1,25(OH)2D) exerts its genomic effects via binding to a nuclear high-affinity vitamin D receptor (VDR). Recent deep sequencing analysis of VDR binding locations across the complete genome has significantly expanded our understanding of the actions of vitamin D and VDR on gene transcription. However, these studies have also promoted appreciation of the extra-transcriptional impact of vitamin D on gene expression. It is now clear that vitamin D interacts with the epigenome via effects on DNA methylation, histone acetylation, and microRNA generation to maintain normal biological functions. There is also increasing evidence that vitamin D can influence pre-mRNA constitutive splicing and alternative splicing, although the mechanism for this remains unclear. Pre-mRNA splicing has long been thought to be a post-transcription RNA processing event, but current data indicate that this occurs co-transcriptionally. Several steroid hormones have been recognized to coordinately control gene transcription and pre-mRNA splicing through the recruitment of nuclear receptor co-regulators that can both control gene transcription and splicing. The current review will discuss this concept with specific reference to vitamin D, and the potential role of heterogeneous nuclear ribonucleoprotein C (hnRNPC), a nuclear factor with an established function in RNA splicing. hnRNPC, has been shown to be involved in the VDR transcriptional complex as a vitamin D-response element-binding protein (VDRE-BP), and may act as a coupling factor linking VDR-directed gene transcription with RNA splicing. In this way hnRNPC may provide an additional mechanism for the fine-tuning of vitamin D-regulated target gene expression. This article is part of a Special Issue entitled '17th Vitamin D Workshop'.

  1. Differential splicing using whole-transcript microarrays

    Directory of Open Access Journals (Sweden)

    Robinson Mark D

    2009-05-01

    Full Text Available Abstract Background The latest generation of Affymetrix microarrays are designed to interrogate expression over the entire length of every locus, thus giving the opportunity to study alternative splicing genome-wide. The Exon 1.0 ST (sense target platform, with versions for Human, Mouse and Rat, is designed primarily to probe every known or predicted exon. The smaller Gene 1.0 ST array is designed as an expression microarray but still interrogates expression with probes along the full length of each well-characterized transcript. We explore the possibility of using the Gene 1.0 ST platform to identify differential splicing events. Results We propose a strategy to score differential splicing by using the auxiliary information from fitting the statistical model, RMA (robust multichip analysis. RMA partitions the probe-level data into probe effects and expression levels, operating robustly so that if a small number of probes behave differently than the rest, they are downweighted in the fitting step. We argue that adjacent poorly fitting probes for a given sample can be evidence of differential splicing and have designed a statistic to search for this behaviour. Using a public tissue panel dataset, we show many examples of tissue-specific alternative splicing. Furthermore, we show that evidence for putative alternative splicing has a strong correspondence between the Gene 1.0 ST and Exon 1.0 ST platforms. Conclusion We propose a new approach, FIRMAGene, to search for differentially spliced genes using the Gene 1.0 ST platform. Such an analysis complements the search for differential expression. We validate the method by illustrating several known examples and we note some of the challenges in interpreting the probe-level data. Software implementing our methods is freely available as an R package.

  2. Using microcomputers for lighting appliance control using a DALI bus

    Directory of Open Access Journals (Sweden)

    Sysala Tomas

    2016-01-01

    Full Text Available Article concerns the DALI bus theoretical and practical aspects. The DALI bus is a modern technology developed especially for illumination systems control. Authors are outlining communication basic principles related to the physical layer and going up to more complex data transactions among particular devices. The article main topic is aimed at two device models development based on microcontroller and exploiting that technology. The first of those two devices is a DALI slave unit enabling the light source or multi switch with light indication components control via DALI bus. The second device measures the ambient illumination intensity and it also detects errors in the load.

  3. Swisstrolley 3 - An articulated trolley bus; SWISSTROLLEY 3. Gelenktrolleybus

    Energy Technology Data Exchange (ETDEWEB)

    Gisler, H.-J.

    2006-07-01

    This illustrated final report for the Swiss Federal Office of Energy (SFOE) takes a look at the Swisstrolley 3 low-floor, articulated trolley bus developed in Switzerland by the Hess AG company. Various features of the variants of the trolley bus, including single vehicle, single and double-articulated buses and bus trailers, are discussed. The reductions achieved in energy consumption, weight and noise are discussed. Typical values for power consumption are presented in graphical form. Economic viability and passenger-friendliness are examined, as are possibilities for further improvement.

  4. Rethinking passive transport: bus fare exemptions and young people's wellbeing.

    Science.gov (United States)

    Jones, Alasdair; Steinbach, Rebecca; Roberts, Helen; Goodman, Anna; Green, Judith

    2012-05-01

    Much recent public health research has emphasised the health impacts for young people of 'active travel' modes, typically defined as walking and cycling. Less research has focused on public transport modes. Drawing on qualitative data, we examine the links between bus travel and wellbeing in London, where young people currently have free bus travel. Our findings indicate that bus travel can be both a physically and socially active experience for young people. We suggest a more nuanced understanding of 'active travel' is now needed, alongside greater attention to urban public transport networks as key sites that impact on important determinants of wellbeing such as independent mobility and social inclusion.

  5. Development in Conducting Bus of RE-Al Alloys

    Institute of Scientific and Technical Information of China (English)

    李德富; 胡捷; 章萍芝; 石瑛; 李彦利

    2001-01-01

    Four kinds of deformed aluminum alloys were tested. One kind of RE-A1-Mg-Si alloy, which possessed high conductivity and strength, was the first priority by contrast test, The large specification bus of conducting aluminum all oys was made by thermomechanical process during the industrial experiment. The properties of the bus are as follows:the tensile strength σb is 238 MPa, the conductivity (v.s. IACS) is 54.3%, and the current-carrying capacity is beyond 3.1kA during working process. This kind of RE-Al alloy bus has been applied to several electrical transmitting and transforming work.

  6. Designing and modelling Havana’s future bus rapid transit

    OpenAIRE

    Warren, James; Ortegon-Sanchez, Adriana

    2015-01-01

    A single bus route in Havana’s bus system is modelled from the current position to a modernised bus rapid transit (BRT). The system is based on an expert-led visioning process and Cuba’s official planning documents, which define the high-level design criteria and their objectives. Building on the experiences of BRT systems that operate in other Latin American cities, a conceptual design for Havana’s BRT system is defined in terms of the key institutional, technical and financial frameworks, a...

  7. Statistical Analysis for the Driving Cycle of Beijing's Bus

    Institute of Scientific and Technical Information of China (English)

    王震坡; 孙逢春; 王军; 孙立清

    2004-01-01

    According to the test data of the driving model for Beijing's bus routes, 9 parameters and the actual values of Beijing bus are confirmed to evaluate the driving cycle, 2 ways of establishing driving cycle model are analyzed, the formula of calculating driving cycle is acquired, and the calculating driving cycle model and the statistical driving cycle model for the buses in Beijing urban areas are set up. This study provides scientific basis for selecting the bus type and confirming the design parameters and the running method in Beijing.

  8. A new MV bus transfer scheme for nuclear power plants

    Directory of Open Access Journals (Sweden)

    Chang Choong-Koo

    2015-01-01

    Full Text Available Fast bus transfer method is the most popular and residual voltage transfer method that is used as a backup in medium voltage buses in general. The use of the advanced technology like open circuit voltage prediction and digital signal processing algorithms can improve the reliability of fast transfer scheme. However, according to the survey results of the recent operation records in nuclear power plants, there were many instances where the fast transfer scheme has failed. To assure bus transfer in any conditions and circumstances, uninterruptible bus transfer scheme utilizing the state of the art medium voltage UPS is discussed and elaborated.

  9. Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

    Directory of Open Access Journals (Sweden)

    Elela Sherif

    2006-01-01

    Full Text Available Abstract Background We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins. In the present study, we have tested the inhibitory activity of RNA oligonucleotides carrying different tail structures. Results We show that an oligonucleotide with a 5' tail containing the human β-globin branch site sequence inhibits the use of the 5' splice site of Bcl-xL, albeit less efficiently than a tail containing binding sites for the hnRNP A1/A2 proteins. A branch site-containing tail positioned at the 3' end of the oligonucleotide also elicited splicing inhibition but not as efficiently as a 5' tail. The interfering activity of a 3' tail was improved by adding a 5' splice site sequence next to the branch site sequence. A 3' tail carrying a Y-shaped branch structure promoted similar splicing interference. The inclusion of branch site or 5' splice site sequences in the Y-shaped 3' tail further improved splicing inhibition. Conclusion Our in vitro results indicate that a variety of tail architectures can be used to elicit splicing interference at low nanomolar concentrations, thereby broadening the scope and the potential impact of this antisense technology.

  10. Bus drivers' mental conditions and their relation to bus passengers' accidents with a focus on the psychological stress concept.

    Science.gov (United States)

    Yamada, Yasuyuki; Mizuno, Motoki; Sugiura, Miyuki; Tanaka, Sumio; Mizuno, Yuki; Yanagiya, Toshio; Hirosawa, Masataka

    2008-06-01

    The purpose of this study was to clarify the psychological factors of bus drivers' instability that were related to bus passengers' accidents according to the hypothesis model based on the stress concept of Lazarus and Folkman (1984). This research was carried out in 2006. Participants of the study were 39 Japanese male bus drivers. Their average age was 40.2 (SD: 11.1). The average duration of employment was 4.5 (SD:6.1) years. A questionnaire was used that was composed of items concerning the frequency of bus passengers' accidents, performance of safe driving, job stressors, stress reaction and recognition from others. Based on the results, a model assuming that stress reaction caused by job stressors disturbed the bus driver's safe driving and was associated with passengers' accidents in the bus was verified to some degree. Especially, melancholy and tired feeling toward passengers showed a strong relation to the passengers' accidents in the bus. This suggested much room for intervention. Moreover, the recognition from others of their job was confirmed to act as a control factor of the stress reaction.

  11. Nanoporosity of Si (100) bars

    Science.gov (United States)

    Novikov, S. N.; Timoshenkov, S. P.; Minaev, V. S.; Goryunova, E. P.; Gerasimenko, N. N.; Smirnov, D. I.

    2016-09-01

    Si(100) samples cut from a typical bar (100 mm in diameter) prepared using industrial technology are studied. Measurements of the electron work function (EWF) show that the size effects in these samples (a reduction in thickness along with a sample's area and the EWF) detected earlier were due to nanostructure porosity that was buried by the technological treatment of a bar's surface. This hidden nanoporosity is assumed to be a manifestation of the secondary crystal structure.

  12. Triply heavy tetraquark states with the $QQ\\bar{Q}\\bar{q}$ configuration

    CERN Document Server

    Chen, Kan; Wu, Jing; Liu, Yan-Rui; Zhu, Shi-Lin

    2016-01-01

    In the framework of the color-magnetic interaction, we systematically investigate the mass splittings of the $QQ\\bar{Q}\\bar{q}$ tetraquark states and estimated their rough masses in this work. These systems include the explicitly exotic states $cc\\bar{b}\\bar{q}$ and $bb\\bar{c}\\bar{q}$ and the hidden exotic states $cc\\bar{c}\\bar{q}$, $cb\\bar{b}\\bar{q}$, $bc\\bar{c}\\bar{q}$, and $bb\\bar{b}\\bar{q}$. If a state around the estimated mass region could be observed, its nature as a genuine tetraquark is favored. The strong decay patterns shown here will be helpful to the experimental search for these exotic states.

  13. Bar Dissolution in Prolate Halos

    CERN Document Server

    Ideta, M; Ideta, Makoto; Hozumi, Shunsuke

    2000-01-01

    The time evolution of barred structures is examined under the influence of the external forces exerted by a spherical halo and by prolate halos. In particular, galaxy disks are placed in the plane including the major axis of prolate halos, whose configuration is often found in cosmological simulations. N-body disks in fixed external halo fields are simulated, so that bars are formed via dynamical instability. In the subsequent evolution, the bars in prolate halos dissolve gradually with time, while the bar pattern in a spherical halo remains almost unchanged to the end of the simulation. The decay times of the bars suggest that they can be destroyed in a time smaller than a Hubble time. Our results indicate that this dissolution process could occur in real barred galaxies, if they are surrounded by massive dark prolate halos, and the configuration adopted here is not unusual from the viewpoint of galaxy formation. For a prolate halo model, an additional simulation that is restricted to two-dimensional in-plan...

  14. Exact and Metaheuristic Approaches for a Bi-Objective School Bus Scheduling Problem

    OpenAIRE

    2015-01-01

    As a class of hard combinatorial optimization problems, the school bus routing problem has received considerable attention in the last decades. For a multi-school system, given the bus trips for each school, the school bus scheduling problem aims at optimizing bus schedules to serve all the trips within the school time windows. In this paper, we propose two approaches for solving the bi-objective school bus scheduling problem: an exact method of mixed integer programming (MIP) and a metaheuri...

  15. Intelligent Energy Management in a Two Power-Bus Vehicle System

    Science.gov (United States)

    2011-08-09

    Dirty Bus Branch 28V Battery Permanent Magnetic DC Motor Field Controlled DC generator Load Clean Bus Branch 28V Battery Bus Bus 19 DISTRIBUTION...Motor Field Controlled DC generator DC Power Supply Electronic Load DC Power Supply (125V, 3A) Permanent Magnetic DC Motor Field Controlled DC generator...Battery Lithium Battery Load Electronic Load Clean Bus Branch Original System Demo System Hydraulic System Permanent DC Motor Alternator Field

  16. Optimization Model for Headway of a Suburban Bus Route

    Directory of Open Access Journals (Sweden)

    Xiaohong Jiang

    2014-01-01

    Full Text Available Due to relatively low passenger demand, headways of suburban bus route are usually longer than those of urban bus route. Actually it is also difficult to balance the benefits between passengers and operators, subject to the service standards from the government. Hence the headway of a suburban bus route is usually determined on the empirical experience of transport planners. To cope with this problem, this paper proposes an optimization model for designing the headways of suburban bus routes by minimizing the operating and user costs. The user costs take into account both the waiting time cost and the crowding cost. The feasibility and validity of the proposed model are shown by applying it to the Route 206 in Jiangning district, Nanjing city of China. Weightages of passengers’ cost and operating cost are further discussed, considering different passenger flows. It is found that the headway and objective function are affected by the weightages largely.

  17. Rfid-Based Automatic Bus Ticketing: Features and Trends

    Science.gov (United States)

    Oudah, A.

    2016-02-01

    Recent advancements in various technologies have made remarkable developments in various fields for public welfare and public transport is one such area. In the near future public bus transport system with advanced technologies like Radio Frequency Identification Device (RFID), GSM, GPS, ZigBee and RF modules will gain spotlight due to their advantage of higher convenience and greater life standards as compared to the conventional bus systems. In this paper, a comprehensive review of all several proposed bus ticketing and bus information methods has been presented in detail. The study brings out improved solution in terms of cost, convenience, user satisfaction and future implementation. The choice of working modules and their efficient performance has been discussed along with the highlighted importance of the need of technology for welfare of common public and visually impaired.

  18. Study on the Bus Real-time Dispatching Model

    Institute of Scientific and Technical Information of China (English)

    YingZou; JianguoLi; JianhuaHuang; ZhenminTang

    2004-01-01

    The real-time dispatching model on bus system is studied in this article. Realtime dispatching can resume the planned schedule quickly, and then to ensure the reliability of the public transport service and to well raise the service quality.

  19. Multi-Gigabit Rate Radiation Hard Bus Project

    Data.gov (United States)

    National Aeronautics and Space Administration — High speed robust very low power radiation hardened bus interconnects are required to accelerate on-board computations, further improve reconfigurability and upgrade...

  20. Bus Passenger Recognition and Track of Video Sequence

    Directory of Open Access Journals (Sweden)

    Donghua Zhou

    2013-06-01

    Full Text Available Study on bus passenger recognition and track of video sequence is a research aiming at realizing making a statistics on passenger flow volume of bus, which not only helps traffic services center rationally schedule vehicles, but also can avoid overload. The system makes machine vision technology and digital image processing technique applied in customer counting of bus. The key questions in the study include effective collection of video image, effective extraction of moving object in sequence images, recognition of moving targets, trace and count of moving objects. The paper makes deep analysis on relevant technology from the perspective of the theory and practical application of the algorithm, and the study makes achievements. The theoretical algorithm for the study on bus passenger recognition and track of video sequence is distinctive. The practical experiments indicate that the system can satisfy the real-time requirements, and can accurately make statistics on the number of passengers, which has great application value.

  1. Global genome splicing analysis reveals an increased number of alternatively spliced genes with aging.

    Science.gov (United States)

    Rodríguez, Sofía A; Grochová, Diana; McKenna, Tomás; Borate, Bhavesh; Trivedi, Niraj S; Erdos, Michael R; Eriksson, Maria

    2016-04-01

    Alternative splicing (AS) is a key regulatory mechanism for the development of different tissues; however, not much is known about changes to alternative splicing during aging. Splicing events may become more frequent and widespread genome-wide as tissues age and the splicing machinery stringency decreases. Using skin, skeletal muscle, bone, thymus, and white adipose tissue from wild-type C57BL6/J male mice (4 and 18 months old), we examined the effect of age on splicing by AS analysis of the differential exon usage of the genome. The results identified a considerable number of AS genes in skeletal muscle, thymus, bone, and white adipose tissue between the different age groups (ranging from 27 to 246 AS genes corresponding to 0.3-3.2% of the total number of genes analyzed). For skin, skeletal muscle, and bone, we included a later age group (28 months old) that showed that the number of alternatively spliced genes increased with age in all three tissues (P aging disease Hutchinson-Gilford progeria syndrome was performed. The results show that expression of the mutant protein, progerin, is associated with an impaired developmental splicing. As progerin accumulates, the number of genes with AS increases compared to in wild-type skin. Our results indicate the existence of a mechanism for increased AS during aging in several tissues, emphasizing that AS has a more important role in the aging process than previously known.

  2. Nuclearly encoded splicing factors implicated in RNA splicing in higher plant organelles.

    Science.gov (United States)

    de Longevialle, Andéol Falcon; Small, Ian D; Lurin, Claire

    2010-07-01

    Plant organelles arose from two independent endosymbiosis events. Throughout evolutionary history, tight control of chloroplasts and mitochondria has been gained by the nucleus, which regulates most steps of organelle genome expression and metabolism. In particular, RNA maturation, including RNA splicing, is highly dependent on nuclearly encoded splicing factors. Most introns in organelles are group II introns, whose catalytic mechanism closely resembles that of the nuclear spliceosome. Plant group II introns have lost the ability to self-splice in vivo and require nuclearly encoded proteins as cofactors. Since the first splicing factor was identified in chloroplasts more than 10 years ago, many other proteins have been shown to be involved in splicing of one or more introns in chloroplasts or mitochondria. These new proteins belong to a variety of different families of RNA binding proteins and provide new insights into ribonucleo-protein complexes and RNA splicing machineries in organelles. In this review, we describe how splicing factors, encoded by the nucleus and targeted to the organelles, take part in post-transcriptional steps in higher plant organelle gene expression. We go on to discuss the potential for these factors to regulate organelle gene expression.

  3. Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation.

    Directory of Open Access Journals (Sweden)

    Daniela Eckert

    2016-01-01

    Full Text Available The genome of the fission yeast Schizosaccharomyces pombe encodes 17 kinases that are essential for cell growth. These include the cell-cycle regulator Cdc2, as well as several kinases that coordinate cell growth, polarity, and morphogenesis during the cell cycle. In this study, we further characterized another of these essential kinases, Prp4, and showed that the splicing of many introns is dependent on Prp4 kinase activity. For detailed characterization, we chose the genes res1 and ppk8, each of which contains one intron of typical size and position. Splicing of the res1 intron was dependent on Prp4 kinase activity, whereas splicing of the ppk8 intron was not. Extensive mutational analyses of the 5' splice site of both genes revealed that proper transient interaction with the 5' end of snRNA U1 governs the dependence of splicing on Prp4 kinase activity. Proper transient interaction between the branch sequence and snRNA U2 was also important. Therefore, the Prp4 kinase is required for recognition and efficient splicing of introns displaying weak exon1/5' splice sites and weak branch sequences.

  4. Examining the Optimal Fuel Type for Urban Bus Operations

    OpenAIRE

    CAULFIELD, BRIAN

    2010-01-01

    PUBLISHED Washington D.C. Public service fleets offer an attractive option for introducing renewable fuels and alternative technologies on a large scale, which allow for the reduction of both greenhouse gas emissions and exhaust air pollutants. This paper ex amines the use of biomethane (bio-CNG) and compressed natural gas (CNG) for part of the bus fleet in Dublin. Dublin is typical of many international urban centres with a large bus fleet; therefore the results...

  5. Car Delay Model near Bus Stops with Mixed Traffic Flow

    OpenAIRE

    Yang Xiaobao; Huan Mei; Gao Ziyou

    2013-01-01

    This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the...

  6. Compressed natural gas bus safety: a quantitative risk assessment.

    Science.gov (United States)

    Chamberlain, Samuel; Modarres, Mohammad

    2005-04-01

    This study assesses the fire safety risks associated with compressed natural gas (CNG) vehicle systems, comprising primarily a typical school bus and supporting fuel infrastructure. The study determines the sensitivity of the results to variations in component failure rates and consequences of fire events. The components and subsystems that contribute most to fire safety risk are determined. Finally, the results are compared to fire risks of the present generation of diesel-fueled school buses. Direct computation of the safety risks associated with diesel-powered vehicles is possible because these are mature technologies for which historical performance data are available. Because of limited experience, fatal accident data for CNG bus fleets are minimal. Therefore, this study uses the probabilistic risk assessment (PRA) approach to model and predict fire safety risk of CNG buses. Generic failure data, engineering judgments, and assumptions are used in this study. This study predicts the mean fire fatality risk for typical CNG buses as approximately 0.23 fatalities per 100-million miles for all people involved, including bus passengers. The study estimates mean values of 0.16 fatalities per 100-million miles for bus passengers only. Based on historical data, diesel school bus mean fire fatality risk is 0.091 and 0.0007 per 100-million miles for all people and bus passengers, respectively. One can therefore conclude that CNG buses are more prone to fire fatality risk by 2.5 times that of diesel buses, with the bus passengers being more at risk by over two orders of magnitude. The study estimates a mean fire risk frequency of 2.2 x 10(-5) fatalities/bus per year. The 5% and 95% uncertainty bounds are 9.1 x 10(-6) and 4.0 x 10(-5), respectively. The risk result was found to be affected most by failure rates of pressure relief valves, CNG cylinders, and fuel piping.

  7. Splicing variants of porcine synphilin-1

    DEFF Research Database (Denmark)

    Larsen, Knud Erik; Madsen, Lone Bruhn; Farajzadeh, Leila;

    2015-01-01

    %) and to mouse (84%) synphilin-1. Three shorter transcript variants of the synphilin-1 gene were identified, all lacking one or more exons. SNCAIP transcripts were detected in most examined organs and tissues and the highest expression was found in brain tissues and lung. Conserved splicing variants and a novel......RNA was investigated by RNAseq. The presented work reports the molecular cloning and characterization of the porcine (Sus scrofa) synphilin-1 cDNA (SNCAIP) and three splice variants hereof. The porcine SNCAIP cDNA codes for a protein (synphilin-1) of 919 amino acids which shows a high similarity to human (90...... splice form of synhilin-1 were found in this study. All synphilin-1 isoforms encoded by the identified transcript variants lack functional domains important for protein degradation....

  8. Field observations of nearshore bar formation

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian;

    2008-01-01

      The formation of an inner nearshore bar was observed during a high-energy event at the sandy beach of Vejers, Denmark. The bar accreted in situ during surf zone conditions and the growth of the bar was associated with the development of a trough landward of the bar. Measurements of hydrodynamics...

  9. Altered PLP1 splicing causes hypomyelination of early myelinating structures

    DEFF Research Database (Denmark)

    Kevelam, Sietske H; Taube, Jennifer R; van Spaendonk, Rosalina M L

    2015-01-01

    : All patients had unusual hemizygous mutations of PLP1 located in exon 3B (one deletion, one missense and two silent), which is spliced out in isoform DM20, or in intron 3 (five mutations). The deletion led to truncation of PLP1, but not DM20. Four mutations were predicted to affect PLP1/DM20...... for causal mutations. In silico analysis of effects of the mutations on splicing and RNA folding was performed. In vitro gene splicing was examined in RNA from patients' fibroblasts and an immortalized immature oligodendrocyte cell line after transfection with mutant minigene splicing constructs. RESULTS...... alternative splicing by creating exonic splicing silencer motifs or new splice donor sites or by affecting the local RNA structure of the PLP1 splice donor site. Four deep intronic mutations were predicted to destabilize a long-distance interaction structure in the secondary PLP1 RNA fragment involved...

  10. A study of alternative splicing in the pig

    DEFF Research Database (Denmark)

    Hillig, Ann-Britt Nygaard; Cirera Salicio, Susanna; Gilchrist, Michael J.;

    2010-01-01

    BACKGROUND: Since at least half of the genes in mammalian genomes are subjected to alternative splicing, alternative pre-mRNA splicing plays an important contribution to the complexity of the mammalian proteome. Expressed sequence tags (ESTs) provide evidence of a great number of possible...... and mouse, we find putative splice variants in about 30% of the contigs with more than 50 ESTs. Based on the criteria that a minimum of two EST sequences confirmed each splice event, a list of 100 genes with the most distinct tissue-specific alternative splice events was generated from the list...... of candidates. To confirm the tissue specificity of the splice events, 10 genes with functional annotation were randomly selected from which 16 individual splice events were chosen for experimental verification by quantitative PCR (qPCR). Six genes were shown to have tissue specific alternatively spliced...

  11. Regulation of mammalian pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    HUI JingYi

    2009-01-01

    In eukaryotes, most protein-coding genes contain introns which are removed by precursor messenger RNA (pre-mRNA) splicing. Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct proteins. Recent genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene expression. Mis-regulation of splicing causes a wide range of human diseases. This review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA splicing. It also discusses emerging directions in the field of alternative splicing.

  12. Regulation of mammalian pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    In eukaryotes,most protein-coding genes contain introns which are removed by precursor messenger RNA(pre-mRNA) splicing.Alternative splicing is a process by which multiple messenger RNAs(mRNAs) are generated from a single pre-mRNA,resulting in functionally distinct proteins.Recent genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene expression.Mis-regulation of splicing causes a wide range of human diseases.This review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA splicing.It also discusses emerging directions in the field of alternative splicing.

  13. Widespread evolutionary conservation of alternatively spliced exons in caenorhabditis

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Penny, David

    2007-01-01

    Alternative splicing (AS) contributes to increased transcriptome and proteome diversity in various eukaryotic lineages. Previous studies showed low levels of conservation of alternatively spliced (cassette) exons within mammals and within dipterans. We report a strikingly different pattern...

  14. Modulation of RNA splicing as a potential treatment for cancer

    OpenAIRE

    Bauman, John A; Kole, Ryszard

    2011-01-01

    Close to 90% of human genes are transcribed into pre-mRNA that undergoes alternative splicing, producing multiple mRNAs and proteins from single genes. This process is largely responsible for human proteome diversity, and about half of genetic disease-causing mutations affect splicing. Splice-switching oligonucleotides (SSOs) comprise an emerging class of antisense therapeutics that modify gene expression by directing pre-mRNA splice site usage. Bauman et al. investigated an SSO that upregula...

  15. SPA: a probabilistic algorithm for spliced alignment.

    Directory of Open Access Journals (Sweden)

    Erik van Nimwegen

    2006-04-01

    Full Text Available Recent large-scale cDNA sequencing efforts show that elaborate patterns of splice variation are responsible for much of the proteome diversity in higher eukaryotes. To obtain an accurate account of the repertoire of splice variants, and to gain insight into the mechanisms of alternative splicing, it is essential that cDNAs are very accurately mapped to their respective genomes. Currently available algorithms for cDNA-to-genome alignment do not reach the necessary level of accuracy because they use ad hoc scoring models that cannot correctly trade off the likelihoods of various sequencing errors against the probabilities of different gene structures. Here we develop a Bayesian probabilistic approach to cDNA-to-genome alignment. Gene structures are assigned prior probabilities based on the lengths of their introns and exons, and based on the sequences at their splice boundaries. A likelihood model for sequencing errors takes into account the rates at which misincorporation, as well as insertions and deletions of different lengths, occurs during sequencing. The parameters of both the prior and likelihood model can be automatically estimated from a set of cDNAs, thus enabling our method to adapt itself to different organisms and experimental procedures. We implemented our method in a fast cDNA-to-genome alignment program, SPA, and applied it to the FANTOM3 dataset of over 100,000 full-length mouse cDNAs and a dataset of over 20,000 full-length human cDNAs. Comparison with the results of four other mapping programs shows that SPA produces alignments of significantly higher quality. In particular, the quality of the SPA alignments near splice boundaries and SPA's mapping of the 5' and 3' ends of the cDNAs are highly improved, allowing for more accurate identification of transcript starts and ends, and accurate identification of subtle splice variations. Finally, our splice boundary analysis on the human dataset suggests the existence of a novel non

  16. Bus Routes, CTran Bus routes, Published in 2007, 1:1200 (1in=100ft) scale, Town of Cary NC.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as...

  17. DIMETHYL ETHER (DME)-FUELED SHUTTLE BUS DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Elana M. Chapman; Shirish Bhide; Jennifer Stefanik; Howard Glunt; Andre L. Boehman; Allen Homan; David Klinikowski

    2003-04-01

    The objectives of this research and demonstration program are to convert a campus shuttle bus to operation on dimethyl ether, a potential ultra-clean alternative diesel fuel. To accomplish this objective, this project includes laboratory evaluation of a fuel conversion strategy, as well as, field demonstration of the DME-fueled shuttle bus. Since DME is a fuel with no lubricity (i.e., it does not possess the lubricating quality of diesel fuel), conventional fuel delivery and fuel injection systems are not compatible with dimethyl ether. Therefore, to operate a diesel engine on DME one must develop a fuel-tolerant injection system, or find a way to provide the necessary lubricity to the DME. In this project, they have chosen the latter strategy in order to achieve the objective with minimal need to modify the engine. Their strategy is to blend DME with diesel fuel, to obtain the necessary lubricity to protect the fuel injection system and to achieve low emissions. The bulk of the efforts over the past year were focused on the conversion of the campus shuttle bus. This process, started in August 2001, took until April 2002 to complete. The process culminated in an event to celebrate the launching of the shuttle bus on DME-diesel operation on April 19, 2002. The design of the system on the shuttle bus was patterned after the system developed in the engine laboratory, but also was subjected to a rigorous failure modes effects analysis (FMEA, referred to by Air Products as a ''HAZOP'' analysis) with help from Dr. James Hansel of Air Products. The result of this FMEA was the addition of layers of redundancy and over-pressure protection to the system on the shuttle bus. The system became operational in February 2002. Preliminary emissions tests and basic operation of the shuttle bus took place at the Pennsylvania Transportation Institute's test track facility near the University Park airport. After modification and optimization of the system on

  18. CROSS-FRONTIER PUBLIC TRANSPORT: Y BUS

    CERN Document Server

    CERN Housing Service

    2002-01-01

    'Y' buses cover the journey between Val Thoiry in France (shopping centre including'Migros' and other stores) and Blandonnet in Switzerland, where they stop outside the 'Jumbo' shop, now renamed 'Carrefour'. In France, the buses connect up Thoiry, Sergy and St. Genis, then cross the border to stop outside CERN, Meyrin, before continuing towards Geneva along the 'Route de Meyrin', stopping outside the 'La Tour' hospital, then at the terminus Blandonnet. The normal fare on French territory with a slight incursion into Switzerland for this purpose as far as CERN, Meyrin, is 1.20 Euros per single journey (ticket valid for one hour). The same amount is charged for any single journey in Switzerland. For example, the cost of a single journey between St. Genis and the 'La Tour' hospital would be 2.40 Euros. The season ticket ('Abonnement de Travail') opens the right to purchase a ticket valid over any period of seven days which entitles the holder to as many journeys as required between the bus stop nearest to the pl...

  19. Effect of bars on the galaxy properties

    Science.gov (United States)

    Vera, Matias; Alonso, Sol; Coldwell, Georgina

    2016-10-01

    Aims: With the aim of assessing the effects of bars on disk galaxy properties, we present an analysis of different characteristics of spiral galaxies with strong bars, weak bars and without bars. Methods: We identified barred galaxies from the Sloan Digital Sky Survey (SDSS). By visual inspection of SDSS images we classified the face-on spiral galaxies brighter than glog (M∗/M⊙) processing, reflected in the significant changes in the physical properties of the host galaxies.

  20. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Penny, David;

    2007-01-01

    Alternative splicing has been reported in various eukaryotic groups including plants, apicomplexans, diatoms, amoebae, animals and fungi. However, whether widespread alternative splicing has evolved independently in the different eukaryotic groups or was inherited from their last common ancestor...

  1. The implications of alternative splicing in the ENCODE protein complement

    DEFF Research Database (Denmark)

    Tress, Michael L.; Martelli, Pier Luigi; Frankish, Adam;

    2007-01-01

    Alternative premessenger RNA splicing enables genes to generate more than one gene product. Splicing events that occur within protein coding regions have the potential to alter the biological function of the expressed protein and even to create new protein functions. Alternative splicing has been...

  2. 46 CFR 111.60-19 - Cable splices.

    Science.gov (United States)

    2010-10-01

    ... with section 25.11 of IEEE 45-2002 (incorporated by reference; see 46 CFR 110.10-1). ... 46 Shipping 4 2010-10-01 2010-10-01 false Cable splices. 111.60-19 Section 111.60-19 Shipping... REQUIREMENTS Wiring Materials and Methods § 111.60-19 Cable splices. (a) A cable must not be spliced in...

  3. 30 CFR 75.603 - Temporary splice of trailing cable.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Temporary splice of trailing cable. 75.603... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.603 Temporary splice of trailing cable. One temporary splice may be made in any trailing cable. Such trailing cable...

  4. Delta-Bar-Delta and directed random search algorithms to study capacitor banks switching overvoltages

    Directory of Open Access Journals (Sweden)

    Sadeghkhani Iman

    2012-01-01

    Full Text Available This paper introduces an approach to analyse transient overvoltages during capacitor banks switching based on artificial neural networks (ANN. Three learning algorithms, delta-bar-delta (DBD, extended delta-bar-delta (EDBD and directed random search (DRS were used to train the ANNs. The ANN training is based on equivalent parameters of the network and therefore, a trained ANN is applicable to every studied system. The developed ANN is trained with extensive simulated results and tested for typical cases. The new algorithms are presented and demonstrated for a partial 39-bus New England test system. The simulated results show the proposed technique can accurately estimate the peak values of switching overvoltages.

  5. The hnRNP 2H9 gene, which is involved in the splicing reaction, is a multiply spliced gene

    DEFF Research Database (Denmark)

    Honoré, B

    2000-01-01

    The hnRNP 2H9 gene products are involved in the splicing process and participate in early heat shock-induced splicing arrest. By combining low/high stringency hybridisation, database search, Northern and Western blotting it is shown that the gene is alternatively spliced into at least six transcr...

  6. Auxiliary splice factor U2AF26 and transcription factor Gfi1 cooperate directly in regulating CD45 alternative splicing.

    NARCIS (Netherlands)

    Heyd, F.; Dam, G.B. ten; Moroy, T.

    2006-01-01

    By alternative splicing, different isoforms of the transmembrane tyrosine phosphatase CD45 are generated that either enhance or limit T cell receptor signaling. We report here that CD45 alternative splicing is regulated by cooperative action of the splice factor U2AF26 and the transcription factor G

  7. Effect of exonic splicing regulation on synonymous codon usage in alternatively spliced exons of Dscam

    Directory of Open Access Journals (Sweden)

    Takahashi Aya

    2009-08-01

    Full Text Available Abstract Background Synonymous codon usage is typically biased towards translationally superior codons in many organisms. In Drosophila, genomic data indicates that translationally optimal codons and splice optimal codons are mostly mutually exclusive, and adaptation to translational efficiency is reduced in the intron-exon boundary regions where potential exonic splicing enhancers (ESEs reside. In contrast to genomic scale analyses on large datasets, a refined study on a well-controlled set of samples can be effective in demonstrating the effects of particular splice-related factors. Down syndrome cell adhesion molecule (Dscam has the largest number of alternatively spliced exons (ASEs known to date, and the splicing frequency of each ASE is accessible from the relative abundance of the transcript. Thus, these ASEs comprise a unique model system for studying the effect of splicing regulation on synonymous codon usage. Results Codon Bias Indices (CBI in the 3' boundary regions were reduced compared to the rest of the exonic regions among 48 and 33 ASEs of exon 6 and 9 clusters, respectively. These regional differences in CBI were affected by splicing frequency and distance from adjacent exons. Synonymous divergence levels between the 3' boundary region and the remaining exonic region of exon 6 ASEs were similar. Additionally, another sensitive comparison of paralogous exonic regions in recently retrotransposed processed genes and their parental genes revealed that, in the former, the differences in CBI between what were formerly the central regions and the boundary regions gradually became smaller over time. Conclusion Analyses of the multiple ASEs of Dscam allowed direct tests of the effect of splice-related factors on synonymous codon usage and provided clear evidence that synonymous codon usage bias is restricted by exonic splicing signals near the intron-exon boundary. A similar synonymous divergence level between the different exonic

  8. Measurement of B0bar -> D(*)0 K(*)0bar Branching Fractions

    CERN Document Server

    Aubert, B; Boutigny, D; Couderc, F; Karyotakis, Yu; Lees, J P; Poireau, V; Tisserand, V; Zghiche, A; Graugès-Pous, E; Palano, A; Pappagallo, M; Chen, J C; Qi, N D; Rong, G; Wang, P; Zhu, Y S; Eigen, G; Ofte, I; Stugu, B; Abrams, G S; Battaglia, M; Best, D S; Brown, D N; Button-Shafer, J; Cahn, R N; Charles, E; Day, C T; Gill, M S; Gritsan, A V; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Mir, L M; Oddone, P J; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Wenzel, W A; Barrett, M; Ford, K E; Harrison, T J; Hart, A J; Hawkes, C M; Morgan, S E; Watson, A T; Fritsch, M; Goetzen, K; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Peters, K; Schröder, T; Steinke, M; Boyd, J T; Burke, J P; Cottingham, W N; Walker, D; Çuhadar-Dönszelmann, T; Fulsom, B G; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Khan, A; Kyberd, P; Saleem, M; Teodorescu, L; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, Yu K; Bondioli, M; Bruinsma, M; Chao, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M A; Mommsen, R K; Röthel, W; Stoker, D P; Abachi, S; Buchanan, C; Foulkes, S D; Gary, J W; Long, O; Shen, B C; Wang, K; Zhang, L; Del Re, D; Hadavand, H K; Hill, E J; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Nesom, G; Schalk, T; Schumm, B A; Seiden, A; Spradlin, P; Williams, D C; Wilson, M G; Albert, J; Chen, E; Dubois-Felsmann, G P; Dvoretskii, A; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Ryd, A; Samuel, A; Andreassen, R; Mancinelli, G; Meadows, B T; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nauenberg, U; Olivas, A; Ruddick, W O; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Chen, A; Eckhart, E A; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Zeng, Q; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Spaan, B; Brandt, T; Klose, V; Lacker, H M; Nogowski, R; Petzold, A; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Grenier, P; Latour, E; Thiebaux, C; Verderi, M; Bard, D J; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cibinetto, G; Luppi, E; Negrini, M; Piemontese, L; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Capra, R; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Brandenburg, G; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bhimji, W; Bowerman, D A; Dauncey, P D; Egede, U; Flack, R L; Gaillard, J R; Nash, J A; Nikolich, M B; Panduro-Vazquez, W; Chai, X; Charles, M J; Mader, W F; Mallik, U; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Schott, G; Arnaud, N; Davier, M; Grosdidier, G; Höcker, A; Le Diberder, F R; Lepeltier, V; Lutz, A M; Oyanguren, A; Petersen, T C; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Stocchi, A; Wang, W F; Wormser, G; Cheng, C H; Lange, D J; Wright, D M; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; George, K A; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; Di Lodovico, F; Menges, W; Sacco, R; Brown, C L; Cowan, G; Flächer, H U; Hopkins, D A; Jackson, P S; McMahon, T R; Ricciardi, S; Salvatore, F; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Kelly, M P; Lafferty, G D; Naisbit, M T; Williams, J C; Yi, J I; Chen, C; Hulsbergen, W D; Jawahery, A; Kovalskyi, D; Lae, C K; Roberts, D A; Simi, G; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Saremi, S; Stängle, H; Willocq, S Y; Cowan, R; Koeneke, K; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Kim, H; Patel, P M; Potter, C T; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Reidy, J; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; Cavallo, N; De Nardo, Gallieno; Fabozzi, F; Gatto, C; Lista, L; Monorchio, D; Paolucci, P; Piccolo, D; Sciacca, C; Baak, M; Bulten, H; Raven, G; Snoek, H L; Jessop, C P; LoSecco, J M; Allmendinger, T; Benelli, G; Gan, K K; Honscheid, K; Hufnagel, D; Jackson, P D; Kagan, H; Kass, R; Pulliam, T; Rahimi, A M; Ter-Antonian, R; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Galeazzi, F; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Benayoun, M; Chauveau, J; David, P; Del Buono, L; La Vaissière, C de; Hamon, O; Hartfiel, B L; John, M J J; Leruste, P; Malcles, J; Ocariz, J; Roos, L; Therin, G; Behera, P K; Gladney, L; Panetta, J; Biasini, M; Covarelli, R; Pioppi, M; Angelini, C; Batignani, G; Bettarini, S; Bucci, F; Calderini, G; Carpinelli, M; Cenci, R; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Mazur, M A; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J; Haire, M; Judd, D; Wagoner, D E; Biesiada, J; Danielson, N; Elmer, P; Lau, Y P; Lü, C; Olsen, J; Smith, A J S; Telnov, A V; Bellini, F; Cavoto, G; D'Orazio, A; Di, E; Marco; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Safai-Tehrani, F; Voena, C; Schröder, H; Waldi, R; Adye, T; De Groot, N; Franek, B; Olaiya, E O; Wilson, F F; Emery, S; Gaidot, A; Ganzhur, S F; Hamel de Monchenault, G; Kozanecki, Witold; Legendre, M; Mayer, B; Vasseur, G; Yéche, C; Zito, M; Park, W; Purohit, M V; Weidemann, A W; Wilson, J R; Allen, M T; Aston, D; Bartoldus, R; Bechtle, P; Berger, N; Boyarski, A M; Claus, R; Coleman, J P; Convery, M R; Cristinziani, M; Dingfelder, J C; Dong, D; Dorfan, J; Dujmic, D; Dunwoodie, W M; Field, R C; Glanzman, T; Gowdy, S J; Halyo, V; Hast, C; Hrynóva, T; Innes, W R; Kelsey, M H; Kim, P; Kocian, M L; Leith, D W G S; Libby, J; Luitz, S; Lüth, V; Lynch, H L; MacFarlane, D B; Marsiske, H; Messner, R; Müller, D R; O'Grady, C P; Ozcan, V E; Perazzo, A; Perl, M; Ratcliff, B N; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Snyder, A; Stelzer, J; Su, D; Sullivan, M K; Suzuki, K; Swain, S K; Thompson, J M; Vavra, J; Van Bakel, N; Weaver, M; Weinstein, A J R; Wisniewski, W J; Wittgen, M; Wright, D H; Yarritu, A K; Yi, K; Young, C C; Burchat, P R; Edwards, A J; Majewski, S A; Petersen, B A; Roat, C; Wilden, L; Ahmed, S; Alam, M S; Bula, R; Ernst, J A; Jain, V; Pan, B; Saeed, M A; Wappler, F R; Zain, S B; Bugg, W; Krishnamurthy, M; Spanier, S M; Eckmann, R; Ritchie, J L; Satpathy, A; Schwitters, R F; Izen, J M; Kitayama, I; Lou, X C; Ye, S; Bianchi, F; Bóna, M; Gallo, F; Gamba, D; Bomben, M; Bosisio, L; Cartaro, C; Cossutti, F; Della Ricca, G; Dittongo, S; Grancagnolo, S; Lanceri, L; Vitale, L; Azzolini, V; Martínez-Vidal, F; Panvini, R S; Banerjee, Sw; Bhuyan, B; Brown, C M; Fortin, D; Hamano, K; Kowalewski, R V; Nugent, I M; Roney, J M; Sobie, R J; Back, J J; Harrison, P F; Latham, T E; Mohanty, G B; Band, H R; Chen, X; Cheng, B; Dasu, S; Datta, M; Eichenbaum, A M; Flood, K T; Graham, M T; Hollar, J J; Johnson, J R; Kutter, P E; Li, H; Liu, R; Mellado, B; Mihályi, A; Mohapatra, A K; Pan, Y; Pierini, M; Prepost, R; Tan, P; Wu, S L; Yu, Z; Neal, H

    2006-01-01

    We present a study of the decays B0bar --> D(*)0 K(*)0bar using a sample of 226 million Y(4S) --> B Bbar decays collected with the BaBar detector at the PEP-II asymmetric-energy e+e-collider at SLAC. We report evidence for the decay of B0 and B0bar mesons to the D*0 K0s final state with an average branching fraction BF(B0-tilde --> D*0 K0bar) := [ BF(B0bar --> D*0 K0bar) + BF(B0 --> D*0 K0) ]/2 = (3.6 +- 1.2 +- 0.3) x 10^-5. Similarly, we measure BF(B0-tilde --> D0 K0bar) := [ BF(B0bar --> D0 K0bar) + BF(B0 --> D0 K0) ]/2 = (5.3 +- 0.7 +- 0.3) x 10^-5 forthe D0 K0s final state. We measure BF(B0bar --> D0 K*0bar) =^(4.0 +- 0.7 +- 0.3) x 10^-5 and set a 90% confidence level upper limit BF(B0bar --> D0bar K*0bar) D0bar K*0bar)/A(B0bar --> D0 K*0bar)| to be less than 0.4 at the 90% confidence level.

  9. Capillary Electrophoresis Analysis of Conventional Splicing Assays

    DEFF Research Database (Denmark)

    de Garibay, Gorka Ruiz; Acedo, Alberto; García-Casado, Zaida;

    2014-01-01

    of these assays is often challenging. Here, we explore this issue by conducting splicing assays in 31 BRCA2 genetic variants. All variants were assessed by RT-PCR followed by capillary electrophoresis and direct sequencing. If assays did not produce clear-cut outputs (Class-2 or Class-5 according to analytical...

  10. Characterization of trans-splicing in Euglenoids.

    Science.gov (United States)

    Frantz, C; Ebel, C; Paulus, F; Imbault, P

    2000-06-01

    We have looked for trans-splicing of nuclear mRNAs in several Euglenoid species. In Cyclidiopsis acus, Phacus curvicauda, Rhabdomonas costata and Menoidium pellucidum we showed that several premRNAs chosen at random are matured by a transsplicing process: we identified SL-RNA genes whose 5' ends (SLs for spliced leader-sequences) were transferred to the 5' extremities of mRNAs. The SL-RNA genes are located on repeated DNA fragments which also encode 5S rRNA in P. curvicauda and C. acus. The potential secondary structures of SL-RNAs are compared to those previously characterized in two other Euglenoids: Euglena gracilis and Entosiphon sulcatum. In another Euglenoid species, Distigma proteus, since none of the mRNAs examined were trans-spliced, it is possible that trans-splicing does not occur. Phylogeny based on 5S rRNA sequences suggests that the species which have, or have had, chloroplasts (E. gracilis, P. curvicauda, C. acus) diverged early from the others.

  11. Alternative-splicing-mediated gene expression

    Science.gov (United States)

    Wang, Qianliang; Zhou, Tianshou

    2014-01-01

    Alternative splicing (AS) is a fundamental process during gene expression and has been found to be ubiquitous in eukaryotes. However, how AS impacts gene expression levels both quantitatively and qualitatively remains to be fully explored. Here, we analyze two common models of gene expression, each incorporating a simple splice mechanism that a pre-mRNA is spliced into two mature mRNA isoforms in a probabilistic manner. In the constitutive expression case, we show that the steady-state molecular numbers of two mature mRNA isoforms follow mutually independent Poisson distributions. In the bursting expression case, we demonstrate that the tail decay of the steady-state distribution for both mature mRNA isoforms that in general are not mutually independent can be characterized by the product of mean burst size and splicing probability. In both cases, we find that AS can efficiently modulate both the variability (measured by variance) and the noise level of the total mature mRNA, and in particular, the latter is always lower than the noise level of the pre-mRNA, implying that AS always reduces the noise. These results altogether reveal that AS is a mechanism of efficiently controlling the gene expression noise.

  12. Jackson Bar Training Structure Study

    Science.gov (United States)

    2015-05-01

    Jackson, AL. Jackson Bar, a sand bar, is located on the left descending bank upstream of a railroad bridge. Located in a bend in the BWT at Jackson, AL...foot-pounds force 1.355818 joules gallons (U.S. liquid ) 3.785412 E-03 cubic meters inches 0.0254 meters miles (nautical) 1,852 meters miles (U.S...left descending bank southwest of Jackson, AL, and upstream of the Norfolk Southern railroad bridge (Figure 1). The railroad bridge is located in the

  13. Signaling behind bars: a role for bar domains

    NARCIS (Netherlands)

    de Kreuk, B.J.

    2012-01-01

    In this thesis we describe several novel components of growth factor receptor and RhoGTPase activation and signaling. We have demonstrated that the F-BAR protein PACSIN2 is an important regulator of Rac1 output and, as a consequence, cell spreading and migration. This study further established the i

  14. Alternative splicing: a pivotal step between eukaryotic transcription and translation.

    Science.gov (United States)

    Kornblihtt, Alberto R; Schor, Ignacio E; Alló, Mariano; Dujardin, Gwendal; Petrillo, Ezequiel; Muñoz, Manuel J

    2013-03-01

    Alternative splicing was discovered simultaneously with splicing over three decades ago. Since then, an enormous body of evidence has demonstrated the prevalence of alternative splicing in multicellular eukaryotes, its key roles in determining tissue- and species-specific differentiation patterns, the multiple post- and co-transcriptional regulatory mechanisms that control it, and its causal role in hereditary disease and cancer. The emerging evidence places alternative splicing in a central position in the flow of eukaryotic genetic information, between transcription and translation, in that it can respond not only to various signalling pathways that target the splicing machinery but also to transcription factors and chromatin structure.

  15. Expression and purification of splicing proteins from mammalian cells.

    Science.gov (United States)

    Allemand, Eric; Hastings, Michelle L

    2014-01-01

    Pre-mRNA splicing is a complex process that is carried out by a large ribonucleoprotein enzyme, termed the spliceosome, which comprises up to 200 proteins. Despite this complexity, the role of individual spliceosomal proteins in the splicing reaction has been successfully investigated using cell-free assays. In many cases, the splicing factor of interest must be expressed and purified in order to study its function in vitro. Posttranslational modifications such as phosphorylation, methylation, acetylation, and ubiquitination of splicing factors are important for activity. Thus, their purification from mammalian cells presents numerous advantages. Here, we describe a method for expression and purification of splicing proteins from mammalian cells.

  16. Approaches to link RNA secondary structures with splicing regulation

    DEFF Research Database (Denmark)

    Plass, Mireya; Eyras, Eduardo

    2014-01-01

    by facilitating or hindering the interaction with factors and small nuclear ribonucleoproteins (snRNPs) that regulate splicing. Moreover, the secondary structure could play a fundamental role in the splicing of yeast species, which lack many of the regulatory splicing factors present in metazoans. This chapter......In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either...

  17. Application of CAN Bus Technology on Zhongtong Bus%CAN总线技术在中通客车上的应用

    Institute of Scientific and Technical Information of China (English)

    赵佳

    2012-01-01

    The application status of CAN bus technology on Zhongtong bus is introduced, the methods of bus data collection and analysis on Zhongtong traditional and new energy bus are discussed and the development direction of CAN bus technology is prospected.%介绍CAN总线技术在中通客车上的应用状况,探讨中通传统客车及新能源客车的总线数据采集和分析方法。

  18. SplicePie: a novel analytical approach for the detection of alternative, non-sequential and recursive splicing.

    Science.gov (United States)

    Pulyakhina, Irina; Gazzoli, Isabella; 't Hoen, Peter A C; Verwey, Nisha; den Dunnen, Johan T; den Dunnen, Johan; Aartsma-Rus, Annemieke; Laros, Jeroen F J

    2015-07-13

    Alternative splicing is a powerful mechanism present in eukaryotic cells to obtain a wide range of transcripts and protein isoforms from a relatively small number of genes. The mechanisms regulating (alternative) splicing and the paradigm of consecutive splicing have recently been challenged, especially for genes with a large number of introns. RNA-Seq, a powerful technology using deep sequencing in order to determine transcript structure and expression levels, is usually performed on mature mRNA, therefore not allowing detailed analysis of splicing progression. Sequencing pre-mRNA at different stages of splicing potentially provides insight into mRNA maturation. Although the number of tools that analyze total and cytoplasmic RNA in order to elucidate the transcriptome composition is rapidly growing, there are no tools specifically designed for the analysis of nuclear RNA (which contains mixtures of pre- and mature mRNA). We developed dedicated algorithms to investigate the splicing process. In this paper, we present a new classification of RNA-Seq reads based on three major stages of splicing: pre-, intermediate- and post-splicing. Applying this novel classification we demonstrate the possibility to analyze the order of splicing. Furthermore, we uncover the potential to investigate the multi-step nature of splicing, assessing various types of recursive splicing events. We provide the data that gives biological insight into the order of splicing, show that non-sequential splicing of certain introns is reproducible and coinciding in multiple cell lines. We validated our observations with independent experimental technologies and showed the reliability of our method. The pipeline, named SplicePie, is freely available at: https://github.com/pulyakhina/splicing_analysis_pipeline. The example data can be found at: https://barmsijs.lumc.nl/HG/irina/example_data.tar.gz.

  19. Deregulation of splicing factors and breast cancer development.

    Science.gov (United States)

    Silipo, Marco; Gautrey, Hannah; Tyson-Capper, Alison

    2015-10-01

    It is well known that many genes implicated in the development and progression of breast cancer undergo aberrant alternative splicing events to produce proteins with pro-cancer properties. These changes in alternative splicing can arise from mutations or single-nucleotide polymorphisms (SNPs) within the DNA sequences of cancer-related genes, which can strongly affect the activity of splicing factors and influence the splice site choice. However, it is important to note that absence of mutations is not sufficient to prevent misleading choices in splice site selection. There is now increasing evidence to demonstrate that the expression profile of ten splicing factors (including SRs and hnRNPs) and eight RNA-binding proteins changes in breast cancer cells compared with normal cells. These modifications strongly influence the alternative splicing pattern of many cancer-related genes despite the absence of any detrimental mutations within their DNA sequences. Thus, a comprehensive assessment of the splicing factor status in breast cancer is important to provide insights into the mechanisms that lead to breast cancer development and metastasis. Whilst most studies focus on mutations that affect alternative splicing in cancer-related genes, this review focuses on splicing factors and RNA-binding proteins that are themselves deregulated in breast cancer and implicated in cancer-related alternative splicing events.

  20. Bus transport network of Shenyang considering competitive and cooperative relationship

    Science.gov (United States)

    Hu, Baoyu; Feng, Shumin; Nie, Cen

    2017-01-01

    Competition and cooperation is a universal phenomenon in bus transport networks (BTNs) because of the shared stations between bus routes. A measuring method is proposed for competitive and cooperative relationship between bus routes. Based on this measurement, we develop a new representation model for BTNs, namely competitive-cooperative space R. This model is applied to investigate empirically bus transport network of Shenyang (BTN-S) from China. We present the histograms of competitive-cooperative coefficients, competitive coefficients and cooperative coefficients to illustrate that competitive and cooperative relationship plays an important role in transporting passengers. The competitive-cooperative situation shows that cooperative relationship holds an absolutely dominant position in BTN-S. To explore the networked characteristics, we present some empirical distributions, for the number of bus stations on a route, the number of shared stations between two routes, degree and weighted degree, competitive strength, and cooperative strength. We also examine the correlations between degree and competitive strength, and between degree and cooperative strength. Besides, we investigate the diversities of competitive strength and cooperative strength in BTN-S. This study can help us to understand the BTN from a deeper level.

  1. Car Delay Model near Bus Stops with Mixed Traffic Flow

    Directory of Open Access Journals (Sweden)

    Yang Xiaobao

    2013-01-01

    Full Text Available This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the stopping buses. The second occurs as buses merge back to the motorized lane. The average car delay is estimated as the sum of the average delay at these two conflict points and the delay resulting from following the slower bicycles that merged into the motorized lane. Data are collected to calibrate and validate the developed model from one site in Beijing. The sensitivity of car delay to various operation conditions is examined. The results show that both bus stream and bicycle stream have significant effects on car delay. At bus volumes above 200 vehicles per hour, the curbside stop design is not appropriate because of the long car delays. It can be replaced by the bus bay design.

  2. Capacity Region of a New Bus Communication Model

    Directory of Open Access Journals (Sweden)

    Zhuojun Zhuang

    2013-02-01

    Full Text Available In this paper, we study a new bus communication model, where two transmitters wish to send their corresponding private messages and a common message to a destination, while they also wish to send the common message to another receiver connected to the same wire. From an information-theoretical point of view, we first study a general case of this new model (with discrete memoryless channels. The capacity region composed of all achievable (R0,R1,R2 triples is determined for this general model, where R1 and R2 are the transmission rates of the private messages and R0 is the transmission rate of the common message. Then, the result is further explained via the Gaussian example. Finally, we give the capacity region for the new bus communication model with additive Gaussian noises and attenuation factors. This new bus communication model captures various communication scenarios, such as the bus systems in vehicles, and the bus type of communication channel in power line communication (PLC networks.

  3. Study of fast transients generated in a GIS bus duct in compressed $SF_6-N_2$ gas mixtures containing lower percentages of $SF_6$

    OpenAIRE

    Singha, S.; Joy, Thomas M; Naidu, MS

    2000-01-01

    This paper deals with the study of Very Fast Transient Overvoltages (VFTO) with $SF_6-N_2$ gas mixture as the insulating medium, but with lower percentages of $SF_6$ in the mixture. The investigations are performed using a laboratory model bus duct. A capacitive voltage sensor was used to measure the peak VFTO and temporal characteristics. Measurements were carried out in $SF_6-N_2$ gas mixtures wherein the concentration of $SF_6$ was varied from 0 to 20% over a pressure range of 1 to 5 bars....

  4. Bars in the Barkor Street

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In daytime,the Barkor Street is bustling with tourists,pious pilgrims and merchants crowded together.Only when darkness falls does the street become tranquil.Nevertheless,the area surrounding the Barkor Street(from Tibetan Hospital compound extending to east Beijing Road)is then revived by various kinds of bars.

  5. Galaxy Zoo: Observing Secular Evolution Through Bars

    CERN Document Server

    Cheung, Edmond; Masters, Karen L; Nichol, Robert C; Bosma, A; Bell, Eric F; Faber, S M; Koo, David C; Lintott, Chris; Melvin, Thomas; Schawinski, Kevin; Skibba, Ramin A; Willett, Kyle W

    2013-01-01

    Observations have shown that there is a connection between the presence of a bar and the properties of a galaxy. In a parallel effort, simulations have shown that this connection is consistent with the theory of bar-driven secular evolution. But observational evidence of bar-driven secular evolution has been sparse. In this paper, we use the Galaxy Zoo 2 dataset to look for evidence of this secular evolution. Our sample consists of 13,295 disk galaxies, with an overall bar fraction of 23.6 +/- 0.4%, of which 1,154 barred galaxies also have bar length measurements. These samples are the largest ever used to study the role of bars in disk galaxy evolution. We characterize bars by the bar likelihood, the likelihood a bar is present in a given galaxy, and the bar length. These two bar properties show interesting correlations with the specific star formation rate and the inner central structure of galaxies. Comparing these observations to state-of-the-art simulations of bar evolution, which include live halos and ...

  6. Sequence-specific flexibility organization of splicing flanking sequence and prediction of splice sites in the human genome.

    Science.gov (United States)

    Zuo, Yongchun; Zhang, Pengfei; Liu, Li; Li, Tao; Peng, Yong; Li, Guangpeng; Li, Qianzhong

    2014-09-01

    More and more reported results of nucleosome positioning and histone modifications showed that DNA structure play a well-established role in splicing. In this study, a set of DNA geometric flexibility parameters originated from molecular dynamics (MD) simulations were introduced to discuss the structure organization around splice sites at the DNA level. The obtained profiles of specific flexibility/stiffness around splice sites indicated that the DNA physical-geometry deformation could be used as an alternative way to describe the splicing junction region. In combination with structural flexibility as discriminatory parameter, we developed a hybrid computational model for predicting potential splicing sites. And the better prediction performance was achieved when the benchmark dataset evaluated. Our results showed that the mechanical deformability character of a splice junction is closely correlated with both the splice site strength and structural information in its flanking sequences.

  7. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    , and regenerative braking for battery charging. It uses a 19.3 kW Ballard PEM fuel cell, will store 12.6 kg of hydrogen at 350 Bar, and includes a 60 kWh battery storage system. The objectives of the project included the following: (a) To advance commercialization of hydrogen-powered transit buses and supporting infrastructure; (b) To provide public outreach and education by showcasing the operation of a 22-foot fuel cell hybrid shuttle bus and Texas first hydrogen fueling infrastructure; and (c) To showcase operation of zero-emissions vehicle for potential transit applications. As mentioned above, the project successfully demonstrated an early vehicle technology, the Ebus plug-in hybrid fuel cell bus, and that success has led to the acquisition of a more advanced vehicle that can take advantage of the same fueling infrastructure. Needed hydrogen station improvements have been identified that will enhance the capabilities of the fueling infrastructure to serve the new bus and to meet the transit agency needs. Over the course of this project, public officials, local government staff, and transit operators were engaged in outreach and education activities that acquainted them with the real world operation of a fuel cell bus and fueling infrastructure. Transit staff members in the Dallas/Ft. Worth region were invited to a workshop in Arlington, Texas at the North Central Texas Council of Governments to participate in a workshop on hydrogen and fuel cells, and to see the fuel cell bus in operation. The bus was trucked to the meeting for this purpose so that participants could see and ride the bus. Austin area transit staff members visited the fueling site in Austin to be briefed on the bus and to participate in a fueling demonstration. This led to further meetings to determine how a fuel cell bus and fueling station could be deployed at Capital Metro Transit. Target urban regions that expressed additional interest during the project in response to the outreach meetings and

  8. Contagion processes on urban bus networks in Indian cities

    CERN Document Server

    Chatterjee, Atanu; Jagannathan, Krishna

    2015-01-01

    Bus transportation is considered as one of the most convenient and cheapest modes of public transportation in Indian cities. Due to their cost-effectiveness and wide reachability, they help a significant portion of the human population in cities to reach their destinations every day. Although from a transportation point of view they have numerous advantages over other modes of public transportation, they also pose a serious threat of contagious diseases spreading throughout the city. The presence of numerous local spatial constraints makes the process and extent of epidemic spreading extremely difficult to predict. Also, majority of the studies have focused on the contagion processes on scale-free network topologies whereas, spatially-constrained real-world networks such as, bus networks exhibit a wide-spectrum of network topology. Therefore, we aim in this study to understand this complex dynamical process of epidemic outbreak and information diffusion on the bus networks for six different Indian cities usin...

  9. Optimal Locations of Bus Stops Connecting Subways near Urban Intersections

    Directory of Open Access Journals (Sweden)

    Yuan Cui

    2015-01-01

    Full Text Available Unsuitable locations of bus stops which provide feeder transportation connecting subways near urban intersections usually lead to the low efficiency of public transport and level of passenger service. A multiobjective optimization model to distribute such stop locations is proposed to attain the shortest total walk distance of passengers and minimum delay time of cars through intersections and travel time of buses. The Pareto frontier and optimal solutions for the proposed model are given by the distance-based and enumerative methods. The Xizhimen bus stop is selected to implement case studies for verifying the validity of the proposed model. The analysis of sensitivity on possible solutions is also carried out in the case studies. The results show that the proposed model is capable of optimizing the locations of bus stops connecting subways near intersections and helpful to improve the level of passengers service and operational efficiency of public transportation.

  10. American Fuel Cell Bus Project Evaluation. Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-09-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB was developed as part of the Federal Transit Administration's (FTA's) National Fuel Cell Bus Program. Through the non-profit consortia CALSTART, a team led by SunLine Transit Agency and BAE Systems developed a new fuel cell electric bus for demonstration. SunLine added two more AFCBs to its fleet in 2014 and another in 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report summarizes the performance results for the buses through June 2015.

  11. Going Smokefree Matters - Bars and Restaurants Infographic

    Data.gov (United States)

    U.S. Department of Health & Human Services — Explore the Going Smokefree Matters – Bars and Restaurants Infographic which outlines key facts related to the effects of secondhand smoke exposure in bars and...

  12. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates.

    Directory of Open Access Journals (Sweden)

    Manuel Irimia

    Full Text Available Alternative splicing is tightly regulated in a spatio-temporal and quantitative manner. This regulation is achieved by a complex interplay between spliceosomal (trans factors that bind to different sequence (cis elements. cis-elements reside in both introns and exons and may either enhance or silence splicing. Differential combinations of cis-elements allows for a huge diversity of overall splicing signals, together comprising a complex 'splicing code'. Many cis-elements have been identified, and their effects on exon inclusion levels demonstrated in reporter systems. However, the impact of interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs on exon inclusion levels in human and chimpanzee. For this purpose, we compiled and studied comprehensive datasets of predicted ESRs, identified by several computational and experimental approaches, as well as microarray data for changes in alternative splicing levels between human and chimpanzee. Surprisingly, we found no association between changes in predicted ESRs and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little or no effect on splicing, and thus interspecific changes at short-time scales may primarily occur in these effectively neutral ESRs. These results underscore the difficulties of using current computational ESR prediction algorithms to identify truly functionally important motifs, and provide a cautionary tale for studies of the effect of SNPs on splicing in human disease.

  13. The influence of Argonaute proteins on alternative RNA splicing.

    Science.gov (United States)

    Batsché, Eric; Ameyar-Zazoua, Maya

    2015-01-01

    Alternative splicing of precursor RNAs is an important process in multicellular species because it impacts several aspects of gene expression: from the increase of protein repertoire to the level of expression. A large body of evidences demonstrates that factors regulating chromatin and transcription impact the outcomes of alternative splicing. Argonaute (AGO) proteins were known to play key roles in the regulation of gene expression at the post-transcriptional level. More recently, their role in the nucleus of human somatic cells has emerged. Here, we will discuss some of the nuclear functions of AGO, with special emphasis on alternative splicing. The AGO-mediated modulation of alternative splicing is based on several properties of these proteins: their binding to transcripts on chromatin and their interactions with many proteins, especially histone tail-modifying enzymes, HP1γ and splicing factors. AGO proteins may favor a decrease in the RNA-polymerase II kinetics at actively transcribed genes leading to the modulation of alternative splicing decisions. They could also influence alternative splicing through their interaction with core components of the splicing machinery and several splicing factors. We will discuss the modes of AGO recruitment on chromatin at active genes. We suggest that long intragenic antisense transcripts (lincRNA) might be an important feature of genes containing splicing events regulated by AGO.

  14. Low back trouble among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1989-01-01

    a questionnaire in 1978 regarding psychosocial factors and health. The prevalence of frequent low back pain was 57%. In a control group of 195 motormen the prevalence was 40%. Standardized Morbidity Ratio for bus drivers discharged from hospital during the period 1978-1984 with the diagnosis lumbar disc...... herniation was 137 compared to all Danish men. These differences were statistically significant. Of psychosocial factors only "the feeling of being mentally unbalanced" was found to be statistically significantly related to subsequent hospital discharge with a LBT-diagnosis. Long seniority as bus driver...

  15. Persecution as a Theme in Isaac Bashevis Singer's The Bus

    Institute of Scientific and Technical Information of China (English)

    Wu Minzhi

    2008-01-01

    Isaac Bashevis Singer is very much concerned about the fate of Jewish people,so he often presents Jews'sufferings in his literary works.The Bus,by means of describing the narrator's experiences during a bus tour through Spain,in different dimensions reinforces the destructive impact of persecution that Jews have gone through.Moreover,he implies such persecution is not merely confined between Jews and the Gentile,but also spreads among the whole human beings who hold differet beliefs.

  16. Development of fiber optic data bus for aircraft

    Science.gov (United States)

    Komouchi, Yutaka; Sueoka, Akira

    1988-02-01

    An account is given of the design, construction, and both ground and flight testing of a star-coupled fiber-optic data bus consisting of an optic coupler, fibers, a connector, and a transmitter/receiver. This system precludes spark/fire hazards and crosstalk problems, while offering very small size and weight for a given capability. The communication protocol for the data bus is of 1 Mbit/sec command response type, and its design attempted to minimize the effect on electronic interfaces as a result of conversion from electrical to fiber-optic buses.

  17. Overview of Multi-DC-Bus Solutions for DC Microgrids

    DEFF Research Database (Denmark)

    Ricchiuto, D.; Mastromauro, R.A.; Liserre, Marco;

    2013-01-01

    DC Microgrids have recently received a lot of attention in the last years due to high penetration of renewable energy sources as well as distributed energy storage systems. In the future DC microgrids could be preferable respect to AC microgrids in terms of redundancy since multi-DC-Bus solutions...... could provide a continuative power supply to the loads. An overview of Multi-DC-Bus solutions is presented in this paper. The performances are compared on the basis of possible DC microgrid configurations, redundancy, different DC voltage levels....

  18. Sustainable transport and the organisation of bus services in Manchester

    DEFF Research Database (Denmark)

    Gudmundsson, Henrik; Sørensen, Claus Hedegaard

    2008-01-01

    “Sustainable transport” has become a headline for transport planning and policy making around the world. While there are many different definitions of what Sustainable Transport means, it commonly includes efforts to improve the environmental performance of transport systems, and to promote...... alternative modes of transport to private cars, such as public transport. In this paper we focus only on the potential of bus transport services in cities for attracting travellers from cars. It is a basic assumption behind this paper that the ability of public bus transport to attract travellers from cars...

  19. While in Shanghai,Enjoy Unigue Bars!

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Today, bars are nothingnew for people who live inmajor cities. Bars have becomeso common that they have toseek a revolution for survival.It seems the only way tosurvive the fierce market competitionis to take the lead in being special. Asa result, some bars are attractingcustomers with unique features.Among them are:

  20. Performance evaluation of UHF RFID technologies for real-time bus recognition in the Taipei Bus Station.

    Science.gov (United States)

    Own, Chung-Ming; Lee, Da-Sheng; Wang, Ti-Ho; Wang, De-Jun; Ting, Yu-Lun

    2013-06-18

    Transport stations such as airports, ports, and railways have adopted blocked-type pathway management to process and control travel systems in a one-directional manner. However, this excludes highway transportation where large buses have great variability and mobility; thus, an instant influx of numerous buses increases risks and complicates station management. Focusing on Taipei Bus Station, this study employed RFID technology to develop a system platform integrated with modern information technology that has numerous characteristics. This modern information technology comprised the following systems: ultra-high frequency (UHF) radio-frequency identification (RFID), ultrasound and license number identification, and backstage graphic controls. In conclusion, the system enabled management, bus companies, and passengers to experience the national bus station's new generation technology, which provides diverse information and synchronization functions. Furthermore, this technology reached a new milestone in the energy-saving and efficiency-increasing performance of Taiwan's buses.

  1. Resolving deconvolution ambiguity in gene alternative splicing

    Directory of Open Access Journals (Sweden)

    Hubbell Earl

    2009-08-01

    Full Text Available Abstract Background For many gene structures it is impossible to resolve intensity data uniquely to establish abundances of splice variants. This was empirically noted by Wang et al. in which it was called a "degeneracy problem". The ambiguity results from an ill-posed problem where additional information is needed in order to obtain an unique answer in splice variant deconvolution. Results In this paper, we analyze the situations under which the problem occurs and perform a rigorous mathematical study which gives necessary and sufficient conditions on how many and what type of constraints are needed to resolve all ambiguity. This analysis is generally applicable to matrix models of splice variants. We explore the proposal that probe sequence information may provide sufficient additional constraints to resolve real-world instances. However, probe behavior cannot be predicted with sufficient accuracy by any existing probe sequence model, and so we present a Bayesian framework for estimating variant abundances by incorporating the prediction uncertainty from the micro-model of probe responsiveness into the macro-model of probe intensities. Conclusion The matrix analysis of constraints provides a tool for detecting real-world instances in which additional constraints may be necessary to resolve splice variants. While purely mathematical constraints can be stated without error, real-world constraints may themselves be poorly resolved. Our Bayesian framework provides a generic solution to the problem of uniquely estimating transcript abundances given additional constraints that themselves may be uncertain, such as regression fit to probe sequence models. We demonstrate the efficacy of it by extensive simulations as well as various biological data.

  2. Splicing variants of porcine synphilin-1

    Directory of Open Access Journals (Sweden)

    Knud Larsen

    2015-09-01

    Full Text Available Parkinson's disease (PD, idiopathic and familial, is characterized by degradation of dopaminergic neurons and the presence of Lewy bodies (LB in the substantia nigra. LBs contain aggregated proteins of which α-synuclein is the major component. The protein synphilin-1 interacts and colocalizes with α-synuclein in LBs. The aim of this study was to isolate and characterize porcine synphilin-1 and isoforms hereof with the future perspective to use the pig as a model for Parkinson's disease. The porcine SNCAIP cDNA was cloned by reverse transcriptase PCR. The spatial expression of SNCAIP mRNA was investigated by RNAseq. The presented work reports the molecular cloning and characterization of the porcine (Sus scrofa synphilin-1 cDNA (SNCAIP and three splice variants hereof. The porcine SNCAIP cDNA codes for a protein (synphilin-1 of 919 amino acids which shows a high similarity to human (90% and to mouse (84% synphilin-1. Three shorter transcript variants of the synphilin-1 gene were identified, all lacking one or more exons. SNCAIP transcripts were detected in most examined organs and tissues and the highest expression was found in brain tissues and lung. Conserved splicing variants and a novel splice form of synhilin-1 were found in this study. All synphilin-1 isoforms encoded by the identified transcript variants lack functional domains important for protein degradation.

  3. Retrieval of missing spliced leader in dinoflagellates.

    Science.gov (United States)

    Zhang, Huan; Lin, Senjie

    2009-01-01

    Spliced leader (SL) trans-splicing has recently been shown to be a common mRNA processing mechanism in dinoflagellates, in which a short (22-nt) sequence, DCCGUAGCCAUUUUGGCUCAAG (D = U, A, or G), is transplanted from the 5'-end of a small non-coding RNA (SL RNA) to the 5' end of mRNA molecules. The widespread existence of the mechanism in dinoflagellates has been demonstrated by detection of this SL (DinoSL) in a wide phylogenetic range of dinoflagellates. Furthermore, the presence of DinoSL in the transcripts of highly diverse groups of nuclear-encoded genes has led us to postulate that SL trans-splicing is universal in dinoflagellate nuclear genome. However, some observations inconsistent to this postulation have been reported, exemplified by a recent article reporting apparent absence of DinoSL in the transcripts of some nuclear-encoded genes in Amphidinium carterae. Absence of SL in these gene transcripts would have important implication on gene regulation in dinoflagellates and utility of DinoSL as a universal dinoflagellate-specific primer to study dinoflagellate transcriptomics. In this study, we re-examined transcripts of these genes and found that all of them actually contained DinoSL. Therefore, results to date are consistent to our initial postulation that DinoSL occurs in all dinoflagellate nuclear-encoded mRNAs.

  4. A new method for splice site prediction based on the sequence patterns of splicing signals and regulatory elements

    Institute of Scientific and Technical Information of China (English)

    SUN ZongXiao; SANG LingJie; JU LiNing; ZHU HuaiQiu

    2008-01-01

    It is of significance for splice site prediction to develop novel algorithms that combine the sequence patterns of regulatory elements such as enhancers and silencers with the patterns of splicing signals. In this paper, a statistical model of splicing signals was built based on the entropy density profile (EDP) method, weight array method (WAM) and κ test; moreover, the model of splicing regulatory elements was developed by an unsupervised self-learning method to detect motifs associated with regulatory elements. With two models incorporated, a multi-level support vector machine (SVM) system was de-vised to perform ab initio prediction for splice sites originating from DNA sequence in eukaryotic ge-home. Results of large scale tests on human genomic splice sites show that the new method achieves a comparative high performance in splice site prediction. The method is demonstrated to be with at least the same level of performance and usually better performance than the existing SpliceScan method based on modeling regulatory elements, and shown to have higher accuracies than the traditional methods with modeling splicing signals such as the GeneSplicer. In particular, the method has evident advantage over splice site prediction for the genes with lower GC content.

  5. The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer.

    Science.gov (United States)

    Qi, Junlin; Su, Shihuang; Mattox, William

    2007-01-01

    The activation of sex-specific alternative splice sites in the Drosophila melanogaster doublesex and fruitless pre-mRNAs has been well studied and depends on the serine-arginine-rich (SR) splicing factors Tra, Tra2, and Rbp1. Little is known, however, about how SR factors negatively regulate splice sites in other RNAs. Here we examine how Tra2 blocks splicing of the M1 intron from its own transcript. We identify an intronic splicing silencer (ISS) adjacent to the M1 branch point that is sufficient to confer Tra2-dependent repression on another RNA. The ISS was found to function independently of its position within the intron, arguing against the idea that bound repressors function by simply interfering with branch point accessibility to general splicing factors. Conserved subelements of the silencer include five short repeated sequences that are required for Tra2 binding but differ from repeated binding sites found in Tra2-dependent splicing enhancers. The ISS also contains a consensus binding site for Rbp1, and this protein was found to facilitate repression of M1 splicing both in vitro and in Drosophila larvae. In contrast to the cooperative binding of SR proteins observed on the doublesex splicing enhancer, we found that Rbp1 and Tra2 bind to the ISS independently through distinct sequences. Our results suggest that functionally synergistic interactions of these SR factors can cause either splicing activation or repression.

  6. TEEPEE DINING & BAR -TELTTARAVINTOLAN PERUSTAMINEN

    OpenAIRE

    Meronen, Pirita

    2015-01-01

    Opinnäytetyönä tein manuaalin Teepee Dining & Bar -telttaravintolan perustamisesta Suomen Teemaravintolat Oy:lle. Seurasin toimeksiantajani etenemistä ravintolan perustamisprosessissa ja raportoin kaikki ravintolan perustamiseen liittyvät vaiheet manuaaliin. Lisäksi kirjasin manuaaliin kaikki tarvittavat yhteyshenkilöt ja luvat, joita tarvitaan ravintolatoiminnan aloittamiseen. Aloitin opinnäytetyöni pohjatyön tiedon keräämisellä ja haastattelin toimeksiantajaani sekä tutustuin ravintola...

  7. Torsional Split Hopkinson Bar Optimization

    Science.gov (United States)

    2012-04-10

    pillow blocks used to mount the incident and transmitter bars are cast iron based- mounted Babbitt -lined bearing split, for 1 in. shaft diameter...Total 1 McMaster-CARR 5911k16 1" Dia, 6" long anodized aluminum shaft $15.38 8 $123.04 2 McMaster-CARR 6359k37 Cast iron base-mounted babbitt

  8. Half pint/Puf68 is required for negative regulation of splicing by the SR splicing factor Transformer2.

    Science.gov (United States)

    Wang, Shanzhi; Wagner, Eric J; Mattox, William

    2013-08-01

    The SR family of proteins plays important regulatory roles in the control of alternative splicing in a wide range of organisms. These factors affect splicing through both positive and negative controls of splice site recognition by pre-spliceosomal factors. Recent studies indicate that the Drosophila SR factor Transformer 2 (Tra2) activates and represses splicing through distinct and separable effector regions of the protein. While the interactions of its Arg-Ser-rich activator region have been well studied, cofactors involved in splicing repression have yet to be found. Here we use a luciferase-based splicing reporter assay to screen for novel proteins necessary for Tra2-dependent repression of splicing. This approach identified Half pint, also known as Puf68, as a co-repressor required for Tra2-mediated autoregulation of the M1 intron. In vivo, Half pint is required for Tra2-dependent repression of M1 splicing but is not necessary for Tra2-dependent activation of doublesex splicing. Further experiments indicate that the effect of Hfp is sequence-specific and that it associates with these target transcripts in cells. Importantly, known M1 splicing regulatory elements are sufficient to sensitize a heterologous intron to Hfp regulation. Two alternative proteins deriving from Hfp transcripts, Hfp68, and Hfp58, were found to be expressed in vivo but differed dramatically in their effect on M1 splicing. Comparison of the cellular localization of these forms in S2 cells revealed that Hfp68 is predominantly localized to the nucleus while Hfp58 is distributed across both the nucleus and cytoplasm. This accords with their observed effects on splicing and suggests that differential compartmentalization may contribute to the specificity of these isoforms. Together, these studies reveal a function for Half pint in splicing repression and demonstrate it to be specifically required for Tra2-dependent intron inclusion.

  9. 46 CFR 112.05-3 - Main-emergency bus-tie.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Main-emergency bus-tie. 112.05-3 Section 112.05-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS General § 112.05-3 Main-emergency bus-tie. Each bus-tie between a main...

  10. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Roy, Scott William

    2009-01-01

    Alternative splicing is tightly regulated in a spatio-temporal and quantitative manner. This regulation is achieved by a complex interplay between spliceosomal (trans) factors that bind to different sequence (cis) elements. cis-elements reside in both introns and exons and may either enhance...... of interspecific differences in these elements on the evolution of alternative splicing levels has not yet been investigated at genomic level. Here we study the effect of interspecific differences in predicted exonic splicing regulators (ESRs) on exon inclusion levels in human and chimpanzee. For this purpose, we...... and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little...

  11. A multi-agent system simulating human splice site recognition.

    Science.gov (United States)

    Vignal, L; Lisacek, F; Quinqueton, J; d'Aubenton-Carafa, Y; Thermes, C

    1999-06-15

    The present paper describes a method detecting splice sites automatically on the basis of sequence data and models of site/signal recognition supported by experimental evidences. The method is designed to simulate splicing and while doing so, track prediction failures, missing information and possibly test correcting hypotheses. Correlations between nucleotides in the splice site regions and the various elements of the acceptor region are evaluated and combined to assess compensating interactions between elements of the splicing machinery. A scanning model of the acceptor region and a model of interaction between the splicing complexes (exon definition model) are also incorporated in the detection process. Subsets of sites presenting deficiencies of several splice site elements could be identified. Further examination of these sites helps to determine lacking elements and refine models.

  12. Splicing modulation therapy in the treatment of genetic diseases

    Directory of Open Access Journals (Sweden)

    Arechavala-Gomeza V

    2014-12-01

    Full Text Available Virginia Arechavala-Gomeza,1 Bernard Khoo,2 Annemieke Aartsma-Rus3 1Neuromuscular Disorders Group, BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain; 2Endocrinology, Division of Medicine, University College London, London, UK; 3Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands All authors contributed equally to this manuscript Abstract: Antisense-mediated splicing modulation is a tool that can be exploited in several ways to provide a potential therapy for rare genetic diseases. This approach is currently being tested in clinical trials for Duchenne muscular dystrophy and spinal muscular atrophy. The present review outlines the versatility of the approach to correct cryptic splicing, modulate alternative splicing, restore the open reading frame, and induce protein knockdown, providing examples of each. Finally, we outline a possible path forward toward the clinical application of this approach for a wide variety of inherited rare diseases. Keywords: splicing, therapy, antisense oligonucleotides, cryptic splicing, alternative splicing

  13. Accumulation of GC donor splice signals in mammals

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2008-07-01

    Full Text Available Abstract The GT dinucleotide in the first two intron positions is the most conserved element of the U2 donor splice signals. However, in a small fraction of donor sites, GT is replaced by GC. A substantial enrichment of GC in donor sites of alternatively spliced genes has been observed previously in human, nematode and Arabidopsis, suggesting that GC signals are important for regulation of alternative splicing. We used parsimony analysis to reconstruct evolution of donor splice sites and inferred 298 GT > GC conversion events compared to 40 GC > GT conversion events in primate and rodent genomes. Thus, there was substantive accumulation of GC donor splice sites during the evolution of mammals. Accumulation of GC sites might have been driven by selection for alternative splicing. Reviewers This article was reviewed by Jerzy Jurka and Anton Nekrutenko. For the full reviews, please go to the Reviewers' Reports section.

  14. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    , and regenerative braking for battery charging. It uses a 19.3 kW Ballard PEM fuel cell, will store 12.6 kg of hydrogen at 350 Bar, and includes a 60 kWh battery storage system. The objectives of the project included the following: (a) To advance commercialization of hydrogen-powered transit buses and supporting infrastructure; (b) To provide public outreach and education by showcasing the operation of a 22-foot fuel cell hybrid shuttle bus and Texas first hydrogen fueling infrastructure; and (c) To showcase operation of zero-emissions vehicle for potential transit applications. As mentioned above, the project successfully demonstrated an early vehicle technology, the Ebus plug-in hybrid fuel cell bus, and that success has led to the acquisition of a more advanced vehicle that can take advantage of the same fueling infrastructure. Needed hydrogen station improvements have been identified that will enhance the capabilities of the fueling infrastructure to serve the new bus and to meet the transit agency needs. Over the course of this project, public officials, local government staff, and transit operators were engaged in outreach and education activities that acquainted them with the real world operation of a fuel cell bus and fueling infrastructure. Transit staff members in the Dallas/Ft. Worth region were invited to a workshop in Arlington, Texas at the North Central Texas Council of Governments to participate in a workshop on hydrogen and fuel cells, and to see the fuel cell bus in operation. The bus was trucked to the meeting for this purpose so that participants could see and ride the bus. Austin area transit staff members visited the fueling site in Austin to be briefed on the bus and to participate in a fueling demonstration. This led to further meetings to determine how a fuel cell bus and fueling station could be deployed at Capital Metro Transit. Target urban regions that expressed additional interest during the project in response to the outreach meetings and

  15. Identification of common genetic variation that modulates alternative splicing.

    Directory of Open Access Journals (Sweden)

    Jeremy Hull

    2007-06-01

    Full Text Available Alternative splicing of genes is an efficient means of generating variation in protein function. Several disease states have been associated with rare genetic variants that affect splicing patterns. Conversely, splicing efficiency of some genes is known to vary between individuals without apparent ill effects. What is not clear is whether commonly observed phenotypic variation in splicing patterns, and hence potential variation in protein function, is to a significant extent determined by naturally occurring DNA sequence variation and in particular by single nucleotide polymorphisms (SNPs. In this study, we surveyed the splicing patterns of 250 exons in 22 individuals who had been previously genotyped by the International HapMap Project. We identified 70 simple cassette exon alternative splicing events in our experimental system; for six of these, we detected consistent differences in splicing pattern between individuals, with a highly significant association between splice phenotype and neighbouring SNPs. Remarkably, for five out of six of these events, the strongest correlation was found with the SNP closest to the intron-exon boundary, although the distance between these SNPs and the intron-exon boundary ranged from 2 bp to greater than 1,000 bp. Two of these SNPs were further investigated using a minigene splicing system, and in each case the SNPs were found to exert cis-acting effects on exon splicing efficiency in vitro. The functional consequences of these SNPs could not be predicted using bioinformatic algorithms. Our findings suggest that phenotypic variation in splicing patterns is determined by the presence of SNPs within flanking introns or exons. Effects on splicing may represent an important mechanism by which SNPs influence gene function.

  16. Pre-mRNA splicing in disease and therapeutics

    OpenAIRE

    Singh, Ravi K; Cooper, Thomas A.

    2012-01-01

    In metazoans, alternative splicing of genes is essential for regulating gene expression and contributing to functional complexity. Computational predictions, comparative genomics, and transcriptome profiling of normal and diseased tissues indicate an unexpectedly high fraction of diseases are caused by mutations that alter splicing. Mutations in cis elements cause mis-splicing of genes that alter gene function and contribute to disease pathology. Mutations of core spliceosomal factors are ass...

  17. Embracing the complexity of pre-mRNA splicing

    Institute of Scientific and Technical Information of China (English)

    Peter J Shepard; Klemens J Hertel

    2010-01-01

    @@ Pre-mRNA splicing is a fundamental process required for the expression of most metazoan genes. It is carried out by the spliceosome, which catalyzes the removal of non-coding intronic sequences to assemble exons into mature mRNAs prior to export and translation.Defects in splicing lead to many human genetic diseases [1], and splicing mutations in a number of genes involved in growth control have been implicated in multiple types of cancer.

  18. Discovery and analysis of evolutionarily conserved intronic splicing regulatory elements.

    Directory of Open Access Journals (Sweden)

    Gene W Yeo

    2007-05-01

    Full Text Available Knowledge of the functional cis-regulatory elements that regulate constitutive and alternative pre-mRNA splicing is fundamental for biology and medicine. Here we undertook a genome-wide comparative genomics approach using available mammalian genomes to identify conserved intronic splicing regulatory elements (ISREs. Our approach yielded 314 ISREs, and insertions of ~70 ISREs between competing splice sites demonstrated that 84% of ISREs altered 5' and 94% altered 3' splice site choice in human cells. Consistent with our experiments, comparisons of ISREs to known splicing regulatory elements revealed that 40%-45% of ISREs might have dual roles as exonic splicing silencers. Supporting a role for ISREs in alternative splicing, we found that 30%-50% of ISREs were enriched near alternatively spliced (AS exons, and included almost all known binding sites of tissue-specific alternative splicing factors. Further, we observed that genes harboring ISRE-proximal exons have biases for tissue expression and molecular functions that are ISRE-specific. Finally, we discovered that for Nova1, neuronal PTB, hnRNP C, and FOX1, the most frequently occurring ISRE proximal to an alternative conserved exon in the splicing factor strongly resembled its own known RNA binding site, suggesting a novel application of ISRE density and the propensity for splicing factors to auto-regulate to associate RNA binding sites to splicing factors. Our results demonstrate that ISREs are crucial building blocks in understanding general and tissue-specific AS regulation and the biological pathways and functions regulated by these AS events.

  19. Effect of bars on the galaxy properties

    CERN Document Server

    Vera, Matias; Coldwell, Georgina

    2016-01-01

    Aims: With the aim of assessing the effects of bars on disc galaxy properties, we present an analysis of different characteristics of spiral galaxies with strong, weak and without bars. Method: We identified barred galaxies from the Sloan Digital Sky Survey. By visual inspection, we classified the face-on spiral galaxies brighter than g<16.5 mag into strong-bar, weak-bar and unbarred. In order to provide an appropiate quantification of the influence of bars on galaxy properties, we also constructed a suitable control sample of unbarred galaxies with similar redshift, magnitude, morphology, bulge sizes, and local density environment distributions to that of barred galaxies. Results: We found 522 strong-barred and 770 weak-barred galaxies, representing a 25.82% of the full sample of spiral galaxies, in good agreement with previous studies. We also found that strong-barred galaxies show less efficient star formation activity and older stellar populations compared to weak-barred and unbarred spirals from the c...

  20. How to Cut Costs by Saving School Bus Fuel.

    Science.gov (United States)

    Seiff, Hank

    A program started in Washington County, Maryland in 1980 has been successful in saving school bus fuel and bringing down transportation costs incurred by its fleet of 200 buses. Driver training and motivation, as well as a partial transfer to diesel buses, are at the heart of the program. The drivers are taught five fuel saving techniques: cut…

  1. Platform Screen Doors Enhanced Bus Rapid Transit Intelligent Performance

    Directory of Open Access Journals (Sweden)

    Chonghua Zhou

    2011-06-01

    Full Text Available It is the last straw to clutch at to solve the urban traffic issue, to develop high-capacity rapid transit and promote transit priority. Characterized by low cost, short construction cycle and flexible development, Bus Rapid Transit (BRT has been favored by more and more cities in the world. The Platform Screen Doors (PSDs system is an important component at BRT stations, and it is original motive to be designed to satisfy the growing demand from BRT application to provide increased safety and comfort in the first. With the continual increased demand for BRT intelligent performance, the PSDs system is applied to get Bus Location Information with accurate position of arrival and departure at stop, to provide Real-Time Information (RTI for BRT passengers using PSDs/GPS compound location technology, to put into practice Bus Fleet Management (BFM. Considering the capability of accurate location, it can be applied to actualize Bus Sign Priority in the future. The authors are luck to take in part the practice of BRT system, especially in the BRT intelligent systems, the papers will introduce upwards application and conceive in detail.

  2. Interface For MIL-STD-1553B Data Bus

    Science.gov (United States)

    Davies, Bryan L.; Osborn, Stephen H.; Sullender, Craig C.

    1993-01-01

    Electronic control-logic subsystem acts as interface between microcontroller and MIL-STD-1553B data bus. Subsystem made of relatively small number of integrated circuits. Advantages include low power, few integrated-circuit chips, and little need for control signals.

  3. Bussikontsern Go Bus ihaldab Harjumaa turgu / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2005-01-01

    AS-i Tarbus ja kaheksa bussiettevõtte ühinemisel moodustati AS Go Bus, ettevõte plaanib veel mitme bussifirma liitmist. Atko Grupi juhatuse esimehe Margo Tomingase, AS Sebe nõukogu esimehe Hugo Osula arvamus. Lisa: Konkurentsiamet hoiab silma peal

  4. Are Good Deeds Compulsory for Young Bus Riders?

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A recent popular post on the Internet concerns friction on a bus in Hangzhou, east China’s Zhejiang Province. A young female passenger surnamed Wu refused to give herseat to a 77-year-old woman when she wasasked to do so by an elderly man surnamed

  5. Bullying on the School Bus: A Video Analysis

    Science.gov (United States)

    Raskauskas, Juliana

    2005-01-01

    The purpose of this study was to assess the nature of bullying on the school bus. Videotapes were used to identify occurrences of bullying on buses of elementary school students. Incidents were reviewed for forms of bullying, fullness, presence of friends, and severity of acts. Results indicated that approximately two incidents occurred per bus…

  6. Measuring Bus Drivers' Occupational Stress Under Changing Working Conditions

    NARCIS (Netherlands)

    Hlotova, Y.; Cats, O.; Meijer, S.A.

    2014-01-01

    Stress is an immense problem in modern society; approximately half of all occupational illnesses are directly or indirectly related to stress. The work of a bus driver is typically associated with high stress levels that negatively influence individual well-being as well as workforce management. The

  7. A Novel Time Synchronization Method for Dynamic Reconfigurable Bus

    Directory of Open Access Journals (Sweden)

    Zhang Weigong

    2016-01-01

    Full Text Available UM-BUS is a novel dynamically reconfigurable high-speed serial bus for embedded systems. It can achieve fault tolerance by detecting the channel status in real time and reconfigure dynamically at run-time. The bus supports direct interconnections between up to eight master nodes and multiple slave nodes. In order to solve the time synchronization problem among master nodes, this paper proposes a novel time synchronization method, which can meet the requirement of time precision in UM-BUS. In this proposed method, time is firstly broadcasted through time broadcast packets. Then, the transmission delay and time deviations via three handshakes during link self-checking and channel detection can be worked out referring to the IEEE 1588 protocol. Thereby, each node calibrates its own time according to the broadcasted time. The proposed method has been proved to meet the requirement of real-time time synchronization. The experimental results show that the synchronous precision can achieve a bias less than 20 ns.

  8. High-Tech School Bus Teaches Students on the Road

    Science.gov (United States)

    Katims, Lauren

    2011-01-01

    Last year, kindergarten through high school students in the rural Hector, Arkansas, School District barely had the technology resources that keep kids interested in math and science. This year, they potentially have the most advanced resources in the country--before they even step into the classroom. One school bus in Arkansas' Pope County has…

  9. Tram of Bus: Does the Tram Bonus Exist?

    NARCIS (Netherlands)

    Bunschoten, T.; Molin, E.J.E.; Van Nes, R.

    2013-01-01

    The tram bonus is a much discussed topic as there is severe uncertainty about its definition and its existence. In this paper the tram bonus is considered to be the extra value it generates for travellers, which causes a new tram service to gain more passengers when compared to an equivalent bus ser

  10. New Constraints on the Galactic Bar

    CERN Document Server

    Minchev, I; Quillen, A C

    2007-01-01

    Previous work has related the Galactic Bar to structure in the local stellar velocity distribution. Here we show that the Bar also influences the spatial gradients of the velocity vector via the Oort constants. By numerical integration of test-particles we simulate measurements of the Oort C value in a gravitational potential including the Galactic Bar. We account for the observed trend that C is increasingly negative for stars with higher velocity dispersion. By comparing measurements of C with our simulations we improve on previous models of the Bar, estimating that the Bar pattern speed is Omega_b/Omega_0=1.87\\pm0.04, where Omega_0 is the local circular frequency, and the Bar angle lies within 20Bar affects measurements of the Oort constants A and B less than ~2 km/s/kpc for the hot stars.

  11. Real life testing of a Hybrid PEM Fuel Cell Bus

    Science.gov (United States)

    Folkesson, Anders; Andersson, Christian; Alvfors, Per; Alaküla, Mats; Overgaard, Lars

    Fuel cells produce low quantities of local emissions, if any, and are therefore one of the most promising alternatives to internal combustion engines as the main power source in future vehicles. It is likely that urban buses will be among the first commercial applications for fuel cells in vehicles. This is due to the fact that urban buses are highly visible for the public, they contribute significantly to air pollution in urban areas, they have small limitations in weight and volume and fuelling is handled via a centralised infrastructure. Results and experiences from real life measurements of energy flows in a Scania Hybrid PEM Fuel Cell Concept Bus are presented in this paper. The tests consist of measurements during several standard duty cycles. The efficiency of the fuel cell system and of the complete vehicle are presented and discussed. The net efficiency of the fuel cell system was approximately 40% and the fuel consumption of the concept bus is between 42 and 48% lower compared to a standard Scania bus. Energy recovery by regenerative braking saves up 28% energy. Bus subsystems such as the pneumatic system for door opening, suspension and brakes, the hydraulic power steering, the 24 V grid, the water pump and the cooling fans consume approximately 7% of the energy in the fuel input or 17% of the net power output from the fuel cell system. The bus was built by a number of companies in a project partly financed by the European Commission's Joule programme. The comprehensive testing is partly financed by the Swedish programme "Den Gröna Bilen" (The Green Car). A 50 kW el fuel cell system is the power source and a high voltage battery pack works as an energy buffer and power booster. The fuel, compressed hydrogen, is stored in two high-pressure stainless steel vessels mounted on the roof of the bus. The bus has a series hybrid electric driveline with wheel hub motors with a maximum power of 100 kW. Hybrid Fuel Cell Buses have a big potential, but there are

  12. Energy Performance Optimization of a Bus for Urban Public Transport

    Directory of Open Access Journals (Sweden)

    Paola Gori

    2013-08-01

    Full Text Available Nowadays many people use public transports in urban centres. Consequently, every day a lot of buses move within cities, trying to ensure the best service to citizens. During the year buses becomecrowded places and using an air conditioning system in constant operation, it tries to ensure a certain condition of comfort for the passengers on board. The aim of this study is to analyse and optimize the energy performance of a bus shell, identifying practical solutions that have not yet been adopted in order to reduce the impact of air conditioning on bus consumption and, therefore, on air pollution. For this reason it was decided to conduct a thermal analysis of a bus for public transport, in order to understandthe behaviour of the bus shell and to deduce possible optimization measures that have not yet been made until now. The analysis was carried out considering the hottest day of July and the coldest day ofJanuary, considering the conditions of operation based on the most common graphics TGM able to define the concentration of traffic city during the 24 hours per day. The study was done using the dynamicsimulation software TRNSYS. With this software it was possible to recreate faithfully the structure of the bus and the external environmental conditions, assessing the impact of different technical solutions for an improvement of internal conditions and a reduction of the cooling capacity required. Because the presence of passengers in public transport is considered like a “benefit” during the winter, the analysis started with the identification of a summer solution and the subsequent evaluation of this solution for the wintertime. The aim of this study was to optimize the bus shell and select the most appropriate solutions. Regarding the transparent surfaces it has been given importance to factors such as the thermal transmittance and the solar gain factor (g-value. Aware of the influence given by the solar radiation on the energy loads, we

  13. GeoBus: sharing science research with schools

    Science.gov (United States)

    Roper, Kathryn; Robinson, Ruth; Moorhouse, Ben

    2016-04-01

    GeoBus (www.geobus.org.uk) is an educational outreach project that was developed in 2012 by the Department of Earth and Environmental Sciences at the University of St Andrews, and it is currently sponsored by industry, NERC, The Crown Estate, and the Scottish Government. The aims of GeoBus are to support the teaching of Earth Science in secondary (middle and high) schools by providing teaching support to schools that have little or no experience in teaching this subject. This is, in part, done through the sharing of new science research outcomes and the experiences of young researchers with school pupils to provide a bridge between industry, higher education institutions, research councils and schools. Since its launch, over 40,000 pupils will have been involved in experiential Earth science learning activities in 190 different schools (over 400 separate visits) across the length and breadth of Scotland: many of these schools are in remote and disadvantaged regions. A new GeoBus project is under development within the Department of Earth Sciences at UCL in London. A key aim of GeoBus is to incorporate new research into our workshops with the main challenge being the development of appropriate resources that incorporate the key learning aims and requirements of the science and geography curricula. GeoBus works closely with researchers, teachers and educational practitioners to tailor the research outcomes to the curricula as much as possible. Over the past four years, GeoBus has developed 17 workshops, 5 challenge events and extensive field trips and each of these activities are trialled and evaluated within the university, and adjustments are made before the activities are delivered in schools. Activities are continually reviewed and further developments are made in response to both teacher and pupil feedback. This critical reflection of the project's success and impact is important to insure a positive and significant contribution to the science learning in

  14. Splice-switching antisense oligonucleotides as therapeutic drugs

    OpenAIRE

    Havens, Mallory A.; Hastings, Michelle L.

    2016-01-01

    Splice-switching oligonucleotides (SSOs) are short, synthetic, antisense, modified nucleic acids that base-pair with a pre-mRNA and disrupt the normal splicing repertoire of the transcript by blocking the RNA–RNA base-pairing or protein–RNA binding interactions that occur between components of the splicing machinery and the pre-mRNA. Splicing of pre-mRNA is required for the proper expression of the vast majority of protein-coding genes, and thus, targeting the process offers a means to manipu...

  15. RNA Splicing: Regulation and Dysregulation in the Heart.

    Science.gov (United States)

    van den Hoogenhof, Maarten M G; Pinto, Yigal M; Creemers, Esther E

    2016-02-01

    RNA splicing represents a post-transcriptional mechanism to generate multiple functional RNAs or proteins from a single transcript. The evolution of RNA splicing is a prime example of the Darwinian function follows form concept. A mutation that leads to a new mRNA (form) that encodes for a new functional protein (function) is likely to be retained, and this way, the genome has gradually evolved to encode for genes with multiple isoforms, thereby creating an enormously diverse transcriptome. Advances in technologies to characterize RNA populations have led to a better understanding of RNA processing in health and disease. In the heart, alternative splicing is increasingly being recognized as an important layer of post-transcriptional gene regulation. Moreover, the recent identification of several cardiac splice factors, such as RNA-binding motif protein 20 and SF3B1, not only provided important insight into the mechanisms underlying alternative splicing but also revealed how these splicing factors impact functional properties of the heart. Here, we review our current knowledge of alternative splicing in the heart, with a particular focus on the major and minor spliceosome, the factors controlling RNA splicing, and the role of alternative splicing in cardiac development and disease.

  16. Evolutionary Insights into RNA trans-Splicing in Vertebrates.

    Science.gov (United States)

    Lei, Quan; Li, Cong; Zuo, Zhixiang; Huang, Chunhua; Cheng, Hanhua; Zhou, Rongjia

    2016-03-10

    Pre-RNA splicing is an essential step in generating mature mRNA. RNA trans-splicing combines two separate pre-mRNA molecules to form a chimeric non-co-linear RNA, which may exert a function distinct from its original molecules. Trans-spliced RNAs may encode novel proteins or serve as noncoding or regulatory RNAs. These novel RNAs not only increase the complexity of the proteome but also provide new regulatory mechanisms for gene expression. An increasing amount of evidence indicates that trans-splicing occurs frequently in both physiological and pathological processes. In addition, mRNA reprogramming based on trans-splicing has been successfully applied in RNA-based therapies for human genetic diseases. Nevertheless, clarifying the extent and evolution of trans-splicing in vertebrates and developing detection methods for trans-splicing remain challenging. In this review, we summarize previous research, highlight recent advances in trans-splicing, and discuss possible splicing mechanisms and functions from an evolutionary viewpoint.

  17. Genome-wide analysis of alternative splicing in Chlamydomonas reinhardtii

    Directory of Open Access Journals (Sweden)

    Thomas Julie

    2010-02-01

    Full Text Available Abstract Background Genome-wide computational analysis of alternative splicing (AS in several flowering plants has revealed that pre-mRNAs from about 30% of genes undergo AS. Chlamydomonas, a simple unicellular green alga, is part of the lineage that includes land plants. However, it diverged from land plants about one billion years ago. Hence, it serves as a good model system to study alternative splicing in early photosynthetic eukaryotes, to obtain insights into the evolution of this process in plants, and to compare splicing in simple unicellular photosynthetic and non-photosynthetic eukaryotes. We performed a global analysis of alternative splicing in Chlamydomonas reinhardtii using its recently completed genome sequence and all available ESTs and cDNAs. Results Our analysis of AS using BLAT and a modified version of the Sircah tool revealed AS of 498 transcriptional units with 611 events, representing about 3% of the total number of genes. As in land plants, intron retention is the most prevalent form of AS. Retained introns and skipped exons tend to be shorter than their counterparts in constitutively spliced genes. The splice site signals in all types of AS events are weaker than those in constitutively spliced genes. Furthermore, in alternatively spliced genes, the prevalent splice form has a stronger splice site signal than the non-prevalent form. Analysis of constitutively spliced introns revealed an over-abundance of motifs with simple repetitive elements in comparison to introns involved in intron retention. In almost all cases, AS results in a truncated ORF, leading to a coding sequence that is around 50% shorter than the prevalent splice form. Using RT-PCR we verified AS of two genes and show that they produce more isoforms than indicated by EST data. All cDNA/EST alignments and splice graphs are provided in a website at http://combi.cs.colostate.edu/as/chlamy. Conclusions The extent of AS in Chlamydomonas that we observed is much

  18. Viral interactions with components of the splicing machinery.

    Science.gov (United States)

    Meyer, F

    2016-01-01

    Eukaryotic genes are often interrupted by stretches of sequence with no protein coding potential or obvious function. After transcription, these interrupting sequences must be removed to give rise to the mature messenger RNA. This fundamental process is called RNA splicing and is achieved by complicated machinery made of protein and RNA that assembles around the RNA to be edited. Viruses also use RNA splicing to maximize their coding potential and economize on genetic space, and use clever strategies to manipulate the splicing machinery to their advantage. This article gives an overview of the splicing process and provides examples of viral strategies that make use of various components of the splicing system to promote their replicative cycle. Representative virus families have been selected to illustrate the interaction with various regulatory proteins and ribonucleoproteins. The unifying theme is fine regulation through protein-protein and protein-RNA interactions with the spliceosome components and associated factors to promote or prevent spliceosome assembly on given splice sites, in addition to a strong influence from cis-regulatory sequences on viral transcripts. Because there is an intimate coupling of splicing with the processes that direct mRNA biogenesis, a description of how these viruses couple the regulation of splicing with the retention or stability of mRNAs is also included. It seems that a unique balance of suppression and activation of splicing and nuclear export works optimally for each family of viruses.

  19. DNA splice site sequences clustering method for conservativeness analysis

    Institute of Scientific and Technical Information of China (English)

    Quanwei Zhang; Qinke Peng; Tao Xu

    2009-01-01

    DNA sequences that are near to splice sites have remarkable conservativeness,and many researchers have contributed to the prediction of splice site.In order to mine the underlying biological knowledge,we analyze the conservativeness of DNA splice site adjacent sequences by clustering.Firstly,we propose a kind of DNA splice site sequences clustering method which is based on DBSCAN,and use four kinds of dissimilarity calculating methods.Then,we analyze the conservative feature of the clustering results and the experimental data set.

  20. THE PREVALENCE OF MUSCULOSKELETAL DISORDERS AMONG BUS DRIVERS IN TRICITY

    Directory of Open Access Journals (Sweden)

    Lalit

    2015-10-01

    Full Text Available Background: Musculoskeletal disorders are widespread in many countries around the world. It has been reported that about 58 percent of the world's population over the age of 10 years spent one third of their life span at work. The population at a high risk include nursing facilities, transportation, mining, food processing, leather tanning, heavy and light manufacturing. Transport workers have been found to be at high risk of developing work related musculoskeletal disorders (WRMSDs. There has been literature evidence regarding the prevalence of musculoskeletal disorders in bus drivers of various cities of different countries. But no study has been done so far in Tricity (Chandigarh, Panchkula and Mohali for the same. The purpose of this study is to investigate the prevalence and characteristics of work related musculoskeletal disorders (WRMSDs among bus drivers of Tricity. Methods: 300 bus drivers were included in the study according to the inclusion and exclusion criteria. The standardized Nordic questionnaire for musculoskeletal disorder and a self administered questionnaire were filled by therapist after the personal interview of each driver. Results: Unpaired t test was used to measure the difference in variable of two groups and Karl Pearson’s correlation coefficient was used to determine the correlation between two entities. In the present study, the subjects were in the age group of 25 to 50 years. Out of the total sample of 300 male bus drivers in Tricity, 159 reported that they had WRMSDs. The prevalence of WRMSDs among bus drivers in Tricity was 53%. In present study, the prevalence of low back pain was highest among the bus drivers that are 30.3%, then neck pain 17.3%, knee pain 14.7%, shoulder 6.3%, ankle and feet 5.7%, upper back 4%, hip and thigh 4%, elbow 1.3% and wrist and hand 1.3%. Thus low back pain, neck pain and knee pain are the most prevalent WRMSDs amongst bus drivers. Conclusions: Work-related biomechanical

  1. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-03-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This report provides the early data results and implementation experience of the AT fuel cell bus since it was placed in service.

  2. Bus Stops Location and Bus Route Planning Using Mean Shift Clustering and Ant Colony in West Jakarta

    Science.gov (United States)

    Supangat, Kenny; Eko Soelistio, Yustinus

    2017-03-01

    Traffic Jam has been a daily problem for people in Jakarta which is one of the busiest city in Indonesia up until now. Even though the official government has tried to reduce the impact of traffic issues by developing a new public transportation which takes up a lot of resources and time, it failed to diminish the problem. The actual concern to this problem actually lies in how people move between places in Jakarta where they always using their own vehicle like cars, and motorcycles that fill most of the street in Jakarta. Among much other public transportations that roams the street of Jakarta, Buses is believed to be an efficient transportation that can move many people at once. However, the location of the bus stop is now have moved to the middle of the main road, and its too far for the nearby residence to access to it. This paper proposes an optimal location of optimal bus stops in West Jakarta that is experimentally proven to have a maximal distance of 350 m. The optimal location is estimated by means of mean shift clustering method while the optimal routes are calculated using Ant Colony algorithm. The bus stops locations rate of error is 0.07% with overall route area of 32 km. Based on our experiments, we believe our proposed bus stop plan can be an interesting alternative to reduce traffic congestion in West Jakarta.

  3. Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing.

    Science.gov (United States)

    Juan, Wen Chun; Roca, Xavier; Ong, S Tiong

    2014-01-01

    Aberrant changes in the expression of the pro-apoptotic protein, BCL-2-like 11 (BIM), can result in either impaired or excessive apoptosis, which can contribute to tumorigenesis and degenerative disorders, respectively. Altering BIM pre-mRNA splicing is an attractive approach to modulate apoptosis because BIM activity is partly determined by the alternative splicing of exons 3 or 4, whereby exon 3-containing transcripts are not apoptotic. Here we identified several cis-acting elements and splicing factors involved in BIM alternative splicing, as a step to better understand the regulation of BIM expression. We analyzed a recently discovered 2,903-bp deletion polymorphism within BIM intron 2 that biased splicing towards exon 3, and which also impaired BIM-dependent apoptosis. We found that this region harbors multiple redundant cis-acting elements that repress exon 3 inclusion. Furthermore, we have isolated a 23-nt intronic splicing silencer at the 3' end of the deletion that is important for excluding exon 3. We also show that PTBP1 and hnRNP C repress exon 3 inclusion, and that downregulation of PTBP1 inhibited BIM-mediated apoptosis. Collectively, these findings start building our understanding of the cis-acting elements and splicing factors that regulate BIM alternative splicing, and also suggest potential approaches to alter BIM splicing for therapeutic purposes.

  4. Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process

    Directory of Open Access Journals (Sweden)

    Katja Meyer

    2015-07-01

    Full Text Available Alternative pre-messenger RNA splicing in higher plants emerges as an important layer of regulation upon exposure to exogenous and endogenous cues. Accordingly, mutants defective in RNA-binding proteins predicted to function in the splicing process show severe phenotypic alterations. Among those are developmental defects, impaired responses to pathogen threat or abiotic stress factors, and misregulation of the circadian timing system. A suite of splicing factors has been identified in the model plant Arabidopsis thaliana. Here we summarize recent insights on how defects in these splicing factors impair plant performance.

  5. [Nutritional characteristics of cereal and peanut bars].

    Science.gov (United States)

    Escobar, B; Estévez, A M; Tepper, A; Aguayo, M

    1998-06-01

    Snack with good nutritional value could play an important role in the physical and mental development of children and teenagers since they show a great preference for them. The tendency is increasing their nutritional value by supplying proteins, carbohydrates, fiber, vitamins and minerals in a balanced form. The purpose of this research was to evaluate the chemical, sensorial and nutritional quality of cereal and peanut bars. Three types of bars using different ratios of oat, wheat germ, peanut, toasted and expanded amaranthus and wheat extrudate were prepared. Bars proximate composition was determined according the AOAC methods, and their acceptability according Hedonic Scale. In the biological assays, rats fed with 10% protein diets, were used to obtain the Protein Efficiency Ratio (PER) Net Protein Ratio (NPR) and Apparent Digestibility (AD). Corrected PER, relative PER, relative AD, PER and NPR values did not showed difference between bars CM1 and CM2 (PER: 2.59-2.57; NPR: 3.99-3.95 respectively); CM3 bar showed a lower quality. There were not differences among bars in relation to AD. CM1 and CM2 bars had a better biological quality of the protein being CM3 bar of lower quality. From a chemical and sensorial point of view CM1 bar shows the highest protein content (14.23%) and acceptability (6.8) and CM2 bar shows a high raw fiber content (2.27%).

  6. The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

    NARCIS (Netherlands)

    Sugliani, M.; Brambilla, V.; Clerkx, E.J.M.; Koornneef, M.; Soppe, W.J.J.

    2010-01-01

    ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3- and ABI3-ß, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-ß transcript a

  7. Measurement of $\\pi^{+/-}, K^{+/-}, p$ and $\\bar{p}$ production in $Z^0 \\to q\\bar{q}, Z^{0} \\to b\\bar{b}$ and $Z^{0} \\to u\\bar{u}, d\\bar{d}, s\\bar{s}$ (Particle Identification with the DELPHI Barrel Ring Imaging Cherenkov Counter)

    CERN Document Server

    Schyns, Emile

    1997-01-01

    Measurement of $\\pi^{+/-}, K^{+/-}, p$ and $\\bar{p}$ production in $Z^0 \\to q\\bar{q}, Z^{0} \\to b\\bar{b}$ and $Z^{0} \\to u\\bar{u}, d\\bar{d}, s\\bar{s}$ (Particle Identification with the DELPHI Barrel Ring Imaging Cherenkov Counter)

  8. A Broad Set of Chromatin Factors Influences Splicing

    Science.gov (United States)

    Allemand, Eric; Myers, Michael P.; Garcia-Bernardo, Jose; Harel-Bellan, Annick; Krainer, Adrian R.; Muchardt, Christian

    2016-01-01

    Several studies propose an influence of chromatin on pre-mRNA splicing, but it is still unclear how widespread and how direct this phenomenon is. We find here that when assembled in vivo, the U2 snRNP co-purifies with a subset of chromatin-proteins, including histones and remodeling complexes like SWI/SNF. Yet, an unbiased RNAi screen revealed that the outcome of splicing is influenced by a much larger variety of chromatin factors not all associating with the spliceosome. The availability of this broad range of chromatin factors impacting splicing further unveiled their very context specific effect, resulting in either inclusion or skipping, depending on the exon under scrutiny. Finally, a direct assessment of the impact of chromatin on splicing using an in vitro co-transcriptional splicing assay with pre-mRNAs transcribed from a nucleosomal template, demonstrated that chromatin impacts nascent pre-mRNP in their competence for splicing. Altogether, our data show that numerous chromatin factors associated or not with the spliceosome can affect the outcome of splicing, possibly as a function of the local chromatin environment that by default interferes with the efficiency of splicing. PMID:27662573

  9. Quantitative regulation of alternative splicing in evolution and development

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Roy, Scott W;

    2009-01-01

    Alternative splicing (AS) is a widespread mechanism with an important role in increasing transcriptome and proteome diversity by generating multiple different products from the same gene. Evolutionary studies of AS have focused primarily on the conservation of alternatively spliced sequences...... layer in complex gene regulatory networks and in the emergence of genetic novelties....

  10. Tissue-specific splicing factor gene expression signatures

    NARCIS (Netherlands)

    A.R. Grosso; A.Q. Gomes (Anita); N.L. Barbosa-Morais (Nuno); S. Caldeira (Sandra); N.P. Thorne (Natalie); G. Grech (Godfrey); M.M. von Lindern (Marieke); M. Carmo-Fonseca (Maria)

    2008-01-01

    textabstractThe alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that tissue-spec

  11. Insights into alternative splicing of sarcomeric genes in the heart.

    Science.gov (United States)

    Weeland, Cornelis J; van den Hoogenhof, Maarten M; Beqqali, Abdelaziz; Creemers, Esther E

    2015-04-01

    Driven by rapidly evolving technologies in next-generation sequencing, alternative splicing has emerged as a crucial layer in gene expression, greatly expanding protein diversity and governing complex biological processes in the cardiomyocyte. At the core of cardiac contraction, the physical properties of the sarcomere are carefully orchestrated through alternative splicing to fit the varying demands on the heart. By the recent discovery of RBM20 and RBM24, two major heart and skeletal muscle-restricted splicing factors, it became evident that alternative splicing events in the heart occur in regulated networks rather than in isolated events. Analysis of knockout mice of these splice factors has shed light on the importance of these fundamental processes in the heart. In this review, we discuss recent advances in our understanding of the role and regulation of alternative splicing in the developing and diseased heart, specifically within the sarcomere. Through various examples (titin, myomesin, troponin T, tropomyosin and LDB3) we illustrate how alternative splicing regulates the functional properties of the sarcomere. Finally, we evaluate opportunities and obstacles to modulate alternative splicing in therapeutic approaches for cardiac disease.

  12. 30 CFR 18.43 - Explosion-proof splice boxes.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Explosion-proof splice boxes. 18.43 Section 18.43 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION... Design Requirements § 18.43 Explosion-proof splice boxes. Internal connections shall be rigidly held...

  13. Installation of Shimming Bars for CYCIAE-100

    Institute of Scientific and Technical Information of China (English)

    LV; Yin-long; WANG; Zhen-hui; ZHONG; Jun-qing; LIU; Geng-shou

    2012-01-01

    <正>The 100 MeV high intensity proton cyclotron, CYCIAE-100, is the most critical equipment of the BRIF project at CIAE. The shimming bars are used to get the isochronous magnetic field. In the process of magnetic mapping and shimming, the bars should be repeated the installed and disassembly for about 10 times. Each time the installation precision must be ensured. The bars installation schedule and quality are directly related to the BRIF project progress.

  14. P{bar P} collider physics

    Energy Technology Data Exchange (ETDEWEB)

    Demarteau, M. [State Univ. of New York, Stony Brook, NY (United States)

    1992-04-01

    A brief introduction to {bar p}p collider physics is given. Selected results from the collider experiments at the CERN S{bar p}pS and the Tevatron collider are described. The emphasis is on experimental aspects of {bar p}p collisions. Minimum bias physics and the production of jets, Intermediate Vector Bosons and heavy flavors is reviewed. The outlook for physics at hadron colliders for the near future is briefly discussed.

  15. Periodic body-and-bar frameworks

    CERN Document Server

    Borcea, Ciprian S; Tanigawa, Shin-ichi

    2011-01-01

    Abstractions of crystalline materials known as periodic body-and-bar frameworks are made of rigid bodies connected by fixed-length bars and subject to the action of a group of translations. In this paper, we give a Maxwell-Laman characterization for generic minimally rigid periodic body-and-bar frameworks. As a consequence we obtain efficient polynomial time algorithms for their recognition based on matroid partition and pebble games.

  16. Pre-mRNA splicing in disease and therapeutics.

    Science.gov (United States)

    Singh, Ravi K; Cooper, Thomas A

    2012-08-01

    In metazoans, alternative splicing of genes is essential for regulating gene expression and contributing to functional complexity. Computational predictions, comparative genomics, and transcriptome profiling of normal and diseased tissues indicate that an unexpectedly high fraction of diseases are caused by mutations that alter splicing. Mutations in cis elements cause missplicing of genes that alter gene function and contribute to disease pathology. Mutations of core spliceosomal factors are associated with hematolymphoid neoplasias, retinitis pigmentosa, and microcephalic osteodysplastic primordial dwarfism type 1 (MOPD1). Mutations in the trans regulatory factors that control alternative splicing are associated with autism spectrum disorder, amyotrophic lateral sclerosis (ALS), and various cancers. In addition to discussing the disorders caused by these mutations, this review summarizes therapeutic approaches that have emerged to correct splicing of individual genes or target the splicing machinery.

  17. Splice Site Mutations in the ATP7A Gene

    DEFF Research Database (Denmark)

    Skjørringe, Tina; Tümer, Zeynep; Møller, Lisbeth Birk

    2011-01-01

    Menkes disease (MD) is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12...... mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation...... to in vivo results. Ninety-six percent of the mutations identified in 45 patients with classical MD were predicted to have a significant effect on splicing, which concurs with the absence of any detectable wild-type transcript in all 19 patients investigated in vivo. Sixty-seven percent of the mutations...

  18. [Statistical analysis of DNA sequences nearby splicing sites].

    Science.gov (United States)

    Korzinov, O M; Astakhova, T V; Vlasov, P K; Roĭtberg, M A

    2008-01-01

    Recognition of coding regions within eukaryotic genomes is one of oldest but yet not solved problems of bioinformatics. New high-accuracy methods of splicing sites recognition are needed to solve this problem. A question of current interest is to identify specific features of nucleotide sequences nearby splicing sites and recognize sites in sequence context. We performed a statistical analysis of human genes fragment database and revealed some characteristics of nucleotide sequences in splicing sites neighborhood. Frequencies of all nucleotides and dinucleotides in splicing sites environment were computed and nucleotides and dinucleotides with extremely high\\low occurrences were identified. Statistical information obtained in this work can be used in further development of the methods of splicing sites annotation and exon-intron structure recognition.

  19. Comparative Analysis of Splice Site Regions by Information Content

    Institute of Scientific and Technical Information of China (English)

    T. Shashi Rekha; Chanchal K. Mitra

    2006-01-01

    We have applied concepts from information theory for a comparative analysis of donor (gt) and acceptor (ag) splice site regions in the genes of five different organisms by calculating their mutual information content (relative entropy) over a selected block of nucleotides. A similar pattern that the information content decreases as the block size increases was observed for both regions in all the organisms studied. This result suggests that the information required for splicing might be contained in the consensus of ~6-8 nt at both regions. We assume from our study that even though the nucleotides are showing some degrees of conservation in the flanking regions of the splice sites, certain level of variability is still tolerated,which leads the splicing process to occur normally even if the extent of base pairing is not fully satisfied. We also suggest that this variability can be compensated by recognizing different splice sites with different spliceosomal factors.

  20. Structural analysis of Aircraft fuselage splice joint

    Science.gov (United States)

    Udaya Prakash, R.; Kumar, G. Raj; Vijayanandh, R.; Senthil Kumar, M.; Ramganesh, T.

    2016-09-01

    In Aviation sector, composite materials and its application to each component are one of the prime factors of consideration due to the high strength to weight ratio, design flexibility and non-corrosive so that the composite materials are widely used in the low weight constructions and also it can be treated as a suitable alternative to metals. The objective of this paper is to estimate and compare the suitability of a composite skin joint in an aircraft fuselage with different joints by simulating the displacement, normal stress, vonmises stress and shear stress with the help of numerical solution methods. The reference Z-stringer component of this paper is modeled by CATIA and numerical simulation is carried out by ANSYS has been used for splice joint presents in the aircraft fuselage with three combinations of joints such as riveted joint, bonded joint and hybrid joint. Nowadays the stringers are using to avoid buckling of fuselage skin, it has joined together by rivets and they are connected end to end by splice joint. Design and static analysis of three-dimensional models of joints such as bonded, riveted and hybrid are carried out and results are compared.

  1. Nuss bar migrations: occurrence and classification

    Energy Technology Data Exchange (ETDEWEB)

    Binkovitz, Lauren E.; Binkovitz, Larry A. [Mayo Clinic, Department of Radiology, Rochester, MN (United States); Zendejas, Benjamin; Moir, Christopher R. [Mayo Clinic, Department of Surgery, Rochester, MN (United States)

    2016-12-15

    Pectus excavatum results from dorsal deviation of the sternum causing narrowing of the anterior-posterior diameter of the chest. It can result in significant cosmetic deformities and cardiopulmonary compromise if severe. The Nuss procedure is a minimally invasive technique that involves placing a thin horizontally oriented metal bar below the dorsal sternal apex for correction of the pectus deformity. To identify the frequency and types of Nuss bar migrations, to present a new categorization of bar migrations, and to present examples of true migrations and pseudomigrations. We retrospectively reviewed the electronic medical records and all pertinent radiologic studies of 311 pediatric patients who underwent a Nuss procedure. We evaluated the frequency and type of bar migrations. Bar migration was demonstrated in 23 of 311 patients (7%) and occurred within a mean period of 26 days after surgery. Bar migrations were subjectively defined as deviation of the bar from the position demonstrated on the immediate postoperative radiographs and categorized as superior, inferior, rotation, lateral or flipped using a new classification system. Sixteen of the 23 migrations required re-operation. Nuss bar migration can be diagnosed with careful evaluation of serial radiographs. Nuss bar migration has a wide variety of appearances and requires exclusion of pseudomigration resulting from changes in patient positioning between radiologic examinations. (orig.)

  2. StarBus+: Distributed Object Middleware Practice for Internet Computing

    Institute of Scientific and Technical Information of China (English)

    Huai-Min Wang; Yu-Feng Wang; Yang-Bin Tang

    2005-01-01

    In the past decade, the booming of Internet challenges the middleware in three aspects: quality of service,balance of changes and stabilization, and across-Internet integration. This paper presents our work on distributed object computing middleware technology for these challenges, as well as the research and development on StarBus+, which is a CORBA standard-compliant middleware suite with the features such as object request broker supporting multi-* quality of service, component model, and integration with Web Service. This paper comprehensively presents the design and characteristics of StarBns+, and demonstrates how StarBus+ is enhanced to address the challenges of Internet computing through three case studies: inter-enterprise integration over Internet, application evolution through dynamic reconfiguration,and great massive information system building. The paper also suggests some research directions which are important for Internet computing.

  3. Implementation of I 2C Master Bus Protocol on FPGA

    Directory of Open Access Journals (Sweden)

    Regu Archana

    2014-10-01

    Full Text Available The focus of this paper is on I 2C (Inter-Integrated Circuit protocol interface between Master Bus protocol and slave. Here we are interfacing between micro-controller and DS1307. I 2C bus protocol sends 8 bit data from micro-controller to DS1307. This module was designed in VHDL and simulated and synthesized using Xilinx ISE Design Suite 14.2. I 2C and optimized for area and power. This concept is widely applicable from any high speed device or low speed device to any low speed device or high speed device. This module acts as a slave for the DS1307 at the same time acts like a master for the micro-controller device which can be considered as a slave. . It can be used to interface low speed peripherals like motherboard, embedded system, mobile phones, set top boxes, DVD, PDA’s or other electronic devices.

  4. Distributed cooperative synchronization strategy for multi-bus microgrids

    DEFF Research Database (Denmark)

    Sun, Yao; Zhong, Chaolu; Hou, Xiaochao

    2017-01-01

    communication breaks down, the primary droop control can still operate robustly. Small signal model of entire system is developed to adjust the parameters of distributed active synchronization controller. Simulation results are presented to verify the effectiveness of the proposed method....... synchronization methods based on centralized communication are very costly and not suitable for multi-bus microgrids that have a large number of distributed generators (DGs). To address this concern, this study presents an active synchronization control strategy based on distributed cooperation technology...... for multi-bus microgrids. The proposed method can reconnect the microgrid in island to UG seamlessly with sparse communication channels. Synchronization correction signals are generated by a voltage controller, which are only transmitted to the leader DGs. Meanwhile, each DG exchanges information with its...

  5. THE DESIGN OF VMEBUS BRIDGE CONTROLLER WITH SHARC BUS

    Institute of Scientific and Technical Information of China (English)

    Wang Min; Wu Shunjun; Su Tao

    2005-01-01

    Targeting at the high expense and inflexibility to realize VMEbus bridge controller by professional Integrated Circuit (IC), this paper presents a scheme of adopting CPLD/FPGA (Complicated Programmable Logic Device/Field Programmable Gate Array) to design bridge controller between VMEbus and local bus. SHARC DSP (Digital Signal Processor) bus is an example. It has functions of nearly entire master/slave interface of VMEbus, and can act as DMA (Direct Memory Access) controller and perform block transfer in DMA or master processor initiative way without length limit. External circuit of the design is very simple. In comparison with special ICs, it has high performance to price ratio and can be easily applied to local buses of other processors with quite a little modification.

  6. Bus Encoder for Crosstalk Avoidance in RLC Modeled Interconnects

    Directory of Open Access Journals (Sweden)

    G. Nagendra Babu

    2012-02-01

    Full Text Available Most of the encoding methods proposed in recent years have dealt with only RC modeled VLSI interconnects. For deep sub micron technologies (DSM, on-chip inductive effects have increased due to faster clock speeds, smaller signal rise times and longer length of on-chip interconnects. All these issues raise the concern for cross talk, propagation delay and power dissipation of overall. Therefore, this research work introduces an efficient Bus Encoder using Bus Inverting (BI method. The proposed design dramatically reduces both cross talk and power dissipation in RLC modeled interconnects which makes it suitable for current high-speed low-power VLSI interconnects. The proposed model demonstrates an overall reduction of power dissipation and cross talk induced delay by 55.43% and 45.87%, respectively.

  7. Technology Update of a Control System Trough Corba Bus

    CERN Document Server

    Bertocchi, A; Buceti, G; Centioli, C; Muzio, D D; Iannone, F; Mazza, G; Panella, M; Vitale, V

    2001-01-01

    After more than 10 years of activity, FTU (Frascati Tokamak Upgrade) has to face the problem of taking advantage of the new hardware and software technology saving all the investments yet done. So, for example, the 20 years old Westinghouse PLCs (communicating only through a serial line) have to be leaved in operation while a web-based tool has to be released for plan monitoring purpose. CORBA bus has been demonstrated to be the answer to set up the communication between old and new hardware and software blocks. On FTU the control system is based on Basestar, a Digital/Compaq software suite. We will describe how the real time database of Basestar is now accessed by Java graphic tools, LabView and, in principle, any package accepting external routines. An account will be also done in the data acquisition area where a Camac serial highway driver has been integrated in the new architecture still through the CORBA bus

  8. Investigation on full distribution CNC system based on SERCOS bus

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A full distribution CNC system based on SERCOS bus is studied in accordance with the limitations of traditional PC-based motion card.The conventional PC-based motion control card is dispersed into several autonomous intelligent servo-control units with the function of servo driver.The autonomous intelligent servocontrol units realize the loop control of position,velocity and current.Interpolation computation is completed in PC and the computational results are transferred to every autonomous intelligent servo-control unit by high speed SERCOS bus.Software or hardware synchronization technology is used to ensure all servomotors are successive and synchronously running.The communication and synchronization technology of SERCOS are also researched and the autonomous intelligent servo-control card is developed byself.Finally,the experiment of circle contour process on a prototype system proves the feasibility.

  9. Health effect of a bus ride in an urban environment

    Energy Technology Data Exchange (ETDEWEB)

    Takano, T.

    1983-07-01

    Health effects on subjects during a bus drive on an urban road were investigated in a real situation. Leukocyte count, adrenalin level in blood and urine, noradrenalin and dopamine in urine, diastolic blood pressure, and index of vascular resistance increased significantly on the drive day compared with the control day. A bus drive on an urban road induced enhanced sympathetic-adreno-medullary activity in real life as a result of the comprehensive effect of various factors. The concentrations of air pollutants were excessively high on the road, and correlated with traffic volume. It was concluded that the interactions between psychogenic, physical nonspecific and physical specific effects should be taken into consideration when evaluating human health effects of air pollution in the urban environment.

  10. Leveraging Bus Mobility to Enable Communications in Urban Areas

    CERN Document Server

    Gaito, Sabrina; Rossi, Gian Paolo

    2011-01-01

    This paper shows that the deployment of an opportunistic network on any public transportation system leads to obtain a scalable and efficient urban communication platform. We use the term Bus Switched Networks (BSNs) to indicate this urban backbone that complements the services of 3G networks and enables to meet the application level requirements for a large class of applications by ensuring high delivery ratio and acceptable delays under different conditions of packet load. We sustain these arguments by providing three contributions. The first contribution is a novel and lightweight probabilistic routing protocol for BSN which we prove to be highly effective in satisfying the loose QoS required by urban-wide delay-tolerant information services. The second contribution is the proposal of URBeS, an analysis platform that, given a specific city served by public transportation, produces real bus mobility traces and traffic analysis for any given routing protocol. The last contribution is an extensive benchmark a...

  11. Design of CAN Bus Application in the Process of Papermaking

    Directory of Open Access Journals (Sweden)

    Kaisheng Zhang

    2013-05-01

    Full Text Available In this study, we have a research of the design of can bus application in the process of papermaking. The design for an intelligent measure and control system based on the technology of CAN bus, is applied to detect and control the concentration of wood-pulp, the temperature of multistage dryer and other parameters in the process of papermaking, in order to meet the control requirements and characters for the study quality and ensure paper even and cohesive under the course of manufacturer. The intelligence of measure and control system has been predominantly researched in the article, including the proposal of intelligent control node and communication model, the design of hardware interface circuit in the intelligent measure and control node and the design of whole system.

  12. Chiral Perturbation Theory and the $\\bar B \\bar B$ Strong Interaction

    CERN Document Server

    Liu, Zhan-Wei; Zhu, Shi-Lin

    2012-01-01

    We have calculated the potentials of the heavy (charmed or bottomed) pseudoscalar mesons up to $O(\\epsilon^2)$ with the heavy meson chiral perturbation theory. We take into account the contributions from the football, triangle, box, and crossed diagrams with the 2$\\phi$ exchange and one-loop corrections to the contact terms. We notice that the total 2$\\phi$-exchange potential alone is attractive in the small momentum region in the channel ${\\bar B \\bar B}^{I=1}$, ${\\bar B_s \\bar B_s}^{I=0}$, or ${\\bar B \\bar B_s}^{I=1/2}$, while repulsive in the channel ${\\bar B \\bar B}^{I=0}$. Hopefully the analytical chiral structures of the potentials may be useful in the extrapolation of the heavy meson interaction from lattice QCD simulation.

  13. Virtual Instrument Based on GPIB Interface Bus1

    Institute of Scientific and Technical Information of China (English)

    Xiao,Ying; Qi,Hanhong

    2004-01-01

    In this paper, we introduced the technique of GPIB businterface. Through the controlling of the GPIB interface board,the computer controlled the current source and the voltmeter, both of which have GPIB bus interface, and a virtual instrument testing system for I~V curve was composed. The virtual instrument front panel and the background graphical control program in Labview environment accomplish virtual instrument testing task along with the hardware system.

  14. City of Chula Vista hydrogen fuel cell bus demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, B.; Bamberger, B.

    1996-10-01

    Hydrogen as an energy carrier and fuel has potential for various uses including electricity, commercial, residential, transportation, and industrial. It is an energy carrier that can be produced from a variety of primary sources and potentially can accomplish these various uses while significantly reducing pollution by substituting for or reducing the use of fossil fuels. One of the most immediate and potentially viable roles for hydrogen as an energy carrier will be its use as a transportation fuel, especially in densely populated urban areas where automotive emissions contribute significantly to air pollution. The Department of Energy`s commitment to research and development of hydrogen as an alternative fuel, and California`s Zero Emission Vehicle (ZEV) requirements, both provide the impetus and favorable circumstance for demonstrating hydrogen as a transportation fuel on an urban bus system. The purpose of this project is to demonstrate the feasibility of using solid polymer fuel cells in a hydrogen-powered electric drive system for an urban transit bus application. Fuel cell buses use hydrogen fuel and oxygen from the air to produce electrical power with the only byproduct being pure water. Proton Exchange Membrane (PEM) fuel cells are proposed for this project. Current evidence suggests that fuel cells, which rely on hydrogen and a process known as proton exchange to generate their power, appear to have an infinite life span. All exhaust pollution is completely eliminated, resulting in a Zero Emission Vehicle (ZEV). An urban bus system offers the potential for developing a market for the production of hydrogen propulsion technology due to extensive vehicular use in densely populated areas experiencing pollution from numerous sources, and because the central garaging facilities or the bus system facilitates fueling and maintenance functions.

  15. MEASURING CUSTOMER’S SATISFACTION ON BUS TRANSPORTATION

    OpenAIRE

    Rabiul Islam; Mohammed S. Chowdhury; Mohammad Sumann Sarker; Salauddin Ahmed

    2014-01-01

    The purpose of the study is to examine the factors measuring customers’ satisfaction, especially the impact of service quality on customers’ satisfaction in public transportation industry in the university town of Sintok located in Kedah province of Malaysia. In order to assess the relationships between service quality and customer satisfaction survey http://www.scientificjournals.org/journals2007/articles/1003.htmdata were collected from 300 Bus commuters. All hypotheses were tes...

  16. BC Transit Fuel Cell Bus Project: Evaluation Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Post, M.

    2014-02-01

    This report evaluates a fuel cell electric bus demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. This evaluation report covers two years of revenue service data on the buses from April 2011 through March 2013.

  17. The Designing Bus for Nuclear Safety Class Controller

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dongil; Lee, Myeongkyun; Yun, Donghwa [PONUTech Co,. Ltd., Research Institute, Ulsan (Korea, Republic of); Ryoo, Kwangki [Hanbat National Univ., Daejeon (Korea, Republic of)

    2013-10-15

    EtherCAT (Ethernet for Control Automation Technology) is based on the IEEE 802.3 standard as one of the communication which is the I/O (Input/Output), sensors and communication function of PLC (Programmable Logic Controller) in industry and factory environment use is increasing. The Nuclear Safety Class Controller implemented by the EtherCAT applied bus can be shown the improving performance of data transmission in the controller.

  18. Nearly deterministic Bell measurement using quantum communication bus

    Science.gov (United States)

    Wang, Jia-Ming; Zhu, Meng-zheng; Wang, Dong; Ye, Liu

    2017-03-01

    We present a scheme to implement Bell states measurement for an arbitrary number of photons by using robust continuous variable coherent modes, called as quantum communication bus (qubus) and weak cross-Kerr nonlinearities. Remarkably, the success probability of our scheme is close to unity, and our scheme does not require any ancillary resource entanglement. Our scheme is likely to yield versatile applications for quantum computation and quantum teleportation.

  19. Capacity models on expressway near a bus bay stop with an access

    Institute of Scientific and Technical Information of China (English)

    张洪宾; 孙小端; 贺玉龙; 宇仁德

    2015-01-01

    To determinate the expressway capacity near a bus bay stop with an access, capacity models on the expressway near a bus stop with an access were developed on the basis of gap acceptance theory and queuing theory. Depending on a bus stop position to an entrance or an exit ramp, the capacity models were developed for four cases. Bus bay stops with overflow and bus bay stops without overflow were considered. A comparison of simulation experiment and model calculation was carried out. Results show that the suggested models have high accuracy and reliability, at bus arrival rate below 60 vehicles per hour (veh/h) or vehicle volumes at the entrance and the exit below 200 passenger cars units per hour (pcu/h), and there are no significant difference in the capacities for four cases. When bus arrival rate is above 240 veh/h, the capacities of all four cases will decline rapidly. With berth number increasing, the increasing of the capacities is no obvious for four cases. As the bus arrival rate and vehicle volumes at the entrance and the exit increase, bus stops located downstream of an entrance and upstream of an exit have a remarkably effect on the capacities. The latter case is much heavier than the former. Those results can be used to traffic design and optimization on urban expressway near a bus stop with an access.

  20. An Optimization Model for the Selection of Bus-Only Lanes in a City.

    Directory of Open Access Journals (Sweden)

    Qun Chen

    Full Text Available The planning of urban bus-only lane networks is an important measure to improve bus service and bus priority. To determine the effective arrangement of bus-only lanes, a bi-level programming model for urban bus lane layout is developed in this study that considers accessibility and budget constraints. The goal of the upper-level model is to minimize the total travel time, and the lower-level model is a capacity-constrained traffic assignment model that describes the passenger flow assignment on bus lines, in which the priority sequence of the transfer times is reflected in the passengers' route-choice behaviors. Using the proposed bi-level programming model, optimal bus lines are selected from a set of candidate bus lines; thus, the corresponding bus lane network on which the selected bus lines run is determined. The solution method using a genetic algorithm in the bi-level programming model is developed, and two numerical examples are investigated to demonstrate the efficacy of the proposed model.

  1. BUS BAY PERFORMANCE AND ITS INFLUENCE ON THE CAPACITY OF ROAD NETWORK IN DAR ES SALAAM

    Directory of Open Access Journals (Sweden)

    Nurdin K. Mushule

    2012-01-01

    Full Text Available One of major problems facing motorists in Dar es Salaam city today is congestion. Bus bays have a significant influence on the capacity of a roadway because they interfere with passing vehicles primarily when buses maneuver to pull into and out of bus bays. Bus bay stops will also interfere with vehicles movement if bus demand exceeds the bus bay capacity resulting in some buses waiting in the travel lane until the buses occupying the bay exit the bay. This paper presents the results of a study which was carried out to evaluate the bus bay performance and its influence on the capacity of the roadway network in the city of Dar es Salaam. The case study area covered 11 bus stops along Morogoro road from Ubungo to Magomeni Mapipa. Capacity of bus bays was studied using procedure outlined in the Transit Capacity and Quality of Service Manual of 2003. This enabled the researcher to determine parameters such as dwell times and clearance times which are major determinants of bus stop capacity. The results indicate that only 18% of the bus bay stops studied did not have adequate capacity to cater for the available demand. 9% did not have adequate capacity during peak hours but the capacity was adequate during off-peak hours. The remaining 73% of bus bay stops possess adequate capacity all the time. Although most bus bay stops studied possess adequate capacity, severe congestion was observed at these locations. This is due to erratic behavior of bus drivers who do not utilize the provided space for them to drop off and pick up passengers. Clearly, this is an area that requires more strict enforcement in order to ease the congestion problem in the city by operating the existing capacity more efficiently."

  2. Evaluation of Bus Vibration Comfort Based on Passenger Crowdsourcing Mode

    Directory of Open Access Journals (Sweden)

    Hong Zhao

    2016-01-01

    Full Text Available Vibration comfort is an important factor affecting the quality of service (QoS of bus. In order to make people involved in supervising bus’s vibration comfort and improve passengers’ riding experience, a novel mode of passenger crowdsourcing is introduced. In this paper, comfort degree of bus vibration is calculated from bus’s vibration signals collected by passengers’ smartphones and sent through WiFi to the Boa web server which shows the vibration comfort on the LCD deployed in bus and maybe trigger alarm lamp when the vibration is beyond the threshold. Three challenges here have been overcome: firstly, space coordinate transformation algorithm is used to solve the constant drift of signals collected; secondly, a low-pass filter is designed to isolate gravity from signals real-timely via limited computing resources; thirdly, an embedded evaluation system is developed according to the calculation procedure specified by criterion ISO 2631-1997. Meanwhile, the model proposed is tested in a practical running environment, the vibration data in whole travel are recorded and analyzed offline. The results show that comfort degree of vibration obtained from the experimental system is identical with the truth, and this mode is proved to be effective.

  3. Violence against metropolitan bus drivers and fare collectors in Brazil.

    Science.gov (United States)

    Assunção, Ada Ávila; Medeiros, Adriane Mesquita de

    2015-01-01

    OBJECTIVE To analyze the correlation between sociodemographic factors and working conditions of bus workers in a metropolitan area and violence against them. METHODS This cross-sectional study used a nonprobabilistic sample estimated according to the number of workers employed in bus companies located in three cities in the Belo Horizonte metropolitan region in 2012 (N = 17,470). Face-to-face interviews were conducted using a digital questionnaire. The factors associated with violence were analyzed in two stages using Poisson regression, according to each level. The magnitude of the association was evaluated using prevalence ratios with robust variance and a statistical significance of 5%, and 95% confidence intervals were obtained. RESULTS The study sample comprised 782 drivers and 691 fare collectors; 45.0% participants reported at least one act of violence in the workplace in the last 12 months, with passengers being predominantly responsible. The age of the bus workers was inversely associated with violence. Chronic diseases, sickness absenteeism, and working conditions were also associated with violence. CONCLUSIONS The findings on the correlation between violence and working conditions are essential for implementing prevention strategies by transportation service managers.

  4. Violence against metropolitan bus drivers and fare collectors in Brazil

    Directory of Open Access Journals (Sweden)

    Ada Ávila Assunção

    2015-01-01

    Full Text Available OBJECTIVE To analyze the correlation between sociodemographic factors and working conditions of bus workers in a metropolitan area and violence against them. METHODS This cross-sectional study used a nonprobabilistic sample estimated according to the number of workers employed in bus companies located in three cities in the Belo Horizonte metropolitan region in 2012 (N = 17,470. Face-to-face interviews were conducted using a digital questionnaire. The factors associated with violence were analyzed in two stages using Poisson regression, according to each level. The magnitude of the association was evaluated using prevalence ratios with robust variance and a statistical significance of 5%, and 95% confidence intervals were obtained. RESULTS The study sample comprised 782 drivers and 691 fare collectors; 45.0% participants reported at least one act of violence in the workplace in the last 12 months, with passengers being predominantly responsible. The age of the bus workers was inversely associated with violence. Chronic diseases, sickness absenteeism, and working conditions were also associated with violence. CONCLUSIONS The findings on the correlation between violence and working conditions are essential for implementing prevention strategies by transportation service managers.

  5. Ring Bus Architecture for a Solid-State Recorder

    Science.gov (United States)

    Walker, W. John; Kopf, Edward; Cox, Brian

    2008-01-01

    A document concisely describes a ring bus architecture for a proposed solid-state recorder (SSR) that would serve as buffer of data to be transmitted from a spacecraft to Earth. This architecture would afford fault tolerance needed for reliable operation in an anticipated high-radiation environment in which traditional SSRs cannot operate reliably. Features of the architecture include one or more controller boards and multiple memory boards interconnected in a ringlike topology. The interconnections would be high-speed serial links complying with the Institute of Electrical and Electronics Engineers (IEEE) standard 1393 (which pertains to a spaceborne fiber-optic data bus). Accordingly, each controller and memory board would be equipped with an IEEE-1393-compliant ring-bus-interface unit. The ringlike topology and the multiplicity of memory boards (and, optionally, of controller boards) would afford the redundancy needed for fault tolerance. Each board would be a fault-containment region. The IEEE 1393 links could be routed so that the SSR would continue to function even in the event of multiple failures. This architecture would also support scalability over a wide range. In a fully redundant configuration, it could accommodate between 1 and 125 memory boards.

  6. Designing Parallel Bus Using Universal Asynchronous Receiver Transmitter

    Directory of Open Access Journals (Sweden)

    Satyandra Sharad

    2013-04-01

    Full Text Available This paper entitled “DESIGNING PARALLEL BUSUSING UNIVERSAL ASYNCHRONOUS RECEIVER TRANSMITTER” is designed to the core of a UART interface module, which includes both receive and transmit modules, and the command parser. This paper will be a viable solution to design parallel buses with the help of UART. In the test bench , there is a RFM(register file model to which we write/read back data from just to check our design .The txt file issues serial inputs to the core and the core outputs parallel data and address in the form of bus. This bus is connected to our RFM (register file model instantiated in the test bench along with the design. This makes easy to retrieve parallel data from serial input. The base of the paper is to use microcontroller along with other components to interface with the physical world. In contrast, most serial communication must first be converted back into parallel form by a universal asynchronous receiver/transmitter (UART before they may be directly connected to a data bus. Both Transmissions (Parallel and Serial are used to connect peripheral devices and enable us to communicate with these devices. The UART core described here is designed using VHDL and implemented on Xilinx Vertex FPGA.

  7. Fuel cell transit bus development & commercialization programs at Gerogetown University

    Energy Technology Data Exchange (ETDEWEB)

    Wimmer, R.; Larkins, J.; Romano, S. [Georgetown Univ., Washington, DC (United States)

    1996-12-31

    Fourteen years ago, Georgetown University (GU) perceived the need for a clean, efficient power systems for transportation that could operate on non-petroleum based fuels. The transit bus application was selected to begin system development. GU recognized the range and recharge constraints of a pure battery powered transit bus. A Fuel Cell power system would circumvent these limitations and, with an on board reformer, accommodate liquid fuel for rapid refueling. Feasibility studies for Fuel Cell power systems for transit buses were conducted with the Los Alamos National Laboratory in 1983. Successful results of this investigation resulted in the DOT/DOE Fuel Cell transit bus development program. The first task was to prove that small Fuel Cell power plants were possible. This was achieved with the Phase I development of two 25 kW Phosphoric Acid Fuel Cell (PAFC) brassboard systems. A liquid cooled version was selected for the Phase II activity in which three 30-foot Fuel Cell powered Test Bed Buses (TBBs) were fabricated. The first of these TBBs was delivered in the spring of 1994. All three of these development vehicles are now in Phase III of the program to conduct testing and evaluation, is conducting operational testing of the buses. The test will involve two fuel cell-operated buses; one with a proton exchange fuel cell and the other with a phosphoric acid fuel cell.

  8. An Electric Bus with a Battery Exchange System

    Directory of Open Access Journals (Sweden)

    Jeongyong Kim

    2015-07-01

    Full Text Available As part of the ongoing effort to be independent of petroleum resources and to be free from pollutant emission issues, various electric vehicles have been developed and tested through their integration with real world systems. In the current paper, yet another application specific EV for public transportation, an electric bus, is introduced and explained with results from the pilot test program which was carried out under real traffic conditions. The main feature of the current system is a battery exchanging mechanism mounted on the roof of the bus. The current configuration certainly requires an externally fabricated battery exchanging robot system that would complement the electric bus for a fully automated battery exchanging process. The major advantage of the current system is the quick re-charging of the electric energy through the physical battery exchange and the possible utilization of the battery exchange station as a mini scale energy storage system for grid system peak power shaving. With the total system solution approach for the public transportation system, it is fully expected to create outstanding business opportunities in number of areas such as battery suppliers, battery exchanging station management, battery leasing and many more.

  9. Safety evaluation of a hydrogen fueled transit bus

    Energy Technology Data Exchange (ETDEWEB)

    Coutts, D.A.; Thomas, J.K.; Hovis, G.L.; Wu, T.T. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1997-12-31

    Hydrogen fueled vehicle demonstration projects must satisfy management and regulator safety expectations. This is often accomplished using hazard and safety analyses. Such an analysis has been completed to evaluate the safety of the H2Fuel bus to be operated in Augusta, Georgia. The evaluation methods and criteria used reflect the Department of Energy`s graded approach for qualifying and documenting nuclear and chemical facility safety. The work focused on the storage and distribution of hydrogen as the bus motor fuel with emphases on the technical and operational aspects of using metal hydride beds to store hydrogen. The safety evaluation demonstrated that the operation of the H2Fuel bus represents a moderate risk. This is the same risk level determined for operation of conventionally powered transit buses in the United States. By the same criteria, private passenger automobile travel in the United States is considered a high risk. The evaluation also identified several design and operational modifications that resulted in improved safety, operability, and reliability. The hazard assessment methodology used in this project has widespread applicability to other innovative operations and systems, and the techniques can serve as a template for other similar projects.

  10. Violence against metropolitan bus drivers and fare collectors in Brazil

    Science.gov (United States)

    Assunção, Ada Ávila; de Medeiros, Adriane Mesquita

    2015-01-01

    OBJECTIVE To analyze the correlation between sociodemographic factors and working conditions of bus workers in a metropolitan area and violence against them. METHODS This cross-sectional study used a nonprobabilistic sample estimated according to the number of workers employed in bus companies located in three cities in the Belo Horizonte metropolitan region in 2012 (N = 17,470). Face-to-face interviews were conducted using a digital questionnaire. The factors associated with violence were analyzed in two stages using Poisson regression, according to each level. The magnitude of the association was evaluated using prevalence ratios with robust variance and a statistical significance of 5%, and 95% confidence intervals were obtained. RESULTS The study sample comprised 782 drivers and 691 fare collectors; 45.0% participants reported at least one act of violence in the workplace in the last 12 months, with passengers being predominantly responsible. The age of the bus workers was inversely associated with violence. Chronic diseases, sickness absenteeism, and working conditions were also associated with violence. CONCLUSIONS The findings on the correlation between violence and working conditions are essential for implementing prevention strategies by transportation service managers. PMID:25741657

  11. Perspective Application of Passive Optical Network with Optimized Bus Topology

    Directory of Open Access Journals (Sweden)

    P. Lafata

    2012-06-01

    Full Text Available Passive optical networks (PONs represent a promising solution for modern access telecommunication networks.These networks are able to meet the increasing demands on transmission rate for demanding multimedia services,while they can offer typical shared transmission speed of 1.25 or 2.5 Gbps. The major role in deploying opticaldistribution networks ODNs plays the maximum attenuable loss, which is caused mainly by passive optical splitters.This paper proposes an innovative application of passive optical networks with optimized bus topology especially forlocal backbone data networks. Due to using only passive components, it is necessary to optimize certain parameters,especially an overall attenuation balance. Considering the possibility of such optimization, the passive optical networkwith optimized bus topology provides several interesting opportunities for specific applications. This paper will presentselected aspects of passive optical networks and splitters with asymmetric splitting ratio. The essential part is focusedon the practical demonstration of their use to optimize the passive optical network with bus topology, which acts as alocal backbone network for structured cabling systems, and for local data networks in large buildings.

  12. Too Much Bar and Not Enough Mitzvah? A Proposed Research Agenda on Bar/Bat Mitzvah

    Science.gov (United States)

    Schoenfeld, Stuart

    2010-01-01

    Jewish educators are understandably interested in research on how bar/bat mitzvah affect Jewish education or research on what Jewish schools have done to avoid the distortions of a focus on bar/bat mitzvah. Research might also focus on the somewhat different and more ambitious topic of the role that bar/bat mitzvah play in contemporary Jewish…

  13. Test of bar window with internal bars free from the glass surfaces

    DEFF Research Database (Denmark)

    Schultz, Jørgen Munthe

    1998-01-01

    A sealed glazing unit with 3 horisontal and 3 vertical bars and a reference glazing without bars have been tested in a guarded hotbox. The difference in measured heat loss coefficient between the two test objects is a measure of the thermal influence of the bars. The difference in heat loss...

  14. On the Relation between Spector's Bar Recursion and Modified Bar Recursion

    DEFF Research Database (Denmark)

    Oliva, Paulo Borges

    2002-01-01

    We introduce a variant of Spector's Bar Recursion in finite types to give a realizability interpretation of the classical axiom of dependent choice allowing for the extraction of witnesses from proofs of Sigma_1 formulas in classical analysis. We also give a bar recursive definition of the fan...... functional and study the relationship of our variant of Bar Recursion with others....

  15. A study of B0bar --> D(*)0 K(*)bar decays

    CERN Document Server

    Aubert, B; Boutigny, D; Couderc, F; Gaillard, J M; Hicheur, A; Karyotakis, Yu; Lees, J P; Tisserand, V; Zghiche, A; Palano, A; Pompili, A; Chen, J C; Qi, N D; Rong, G; Wang, P; Zhu, Y S; Eigen, G; Ofte, I; Stugu, B; Abrams, G S; Borgland, A W; Breon, A B; Brown, D N; Button-Shafer, J; Cahn, R N; Charles, E; Day, C T; Gill, M S; Gritsan, A V; Groysman, Y; Jacobsen, R G; Kadel, R W; Kadyk, J; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Mir, L M; Oddone, P J; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, Michael T; Shelkov, V G; Wenzel, W A; Barrett, M; Ford, K E; Harrison, T J; Hart, A J; Hawkes, C M; Morgan, S E; Watson, A T; Fritsch, M; Goetzen, K; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Steinke, M; Boyd, J T; Chevalier, N; Cottingham, W N; Kelly, M P; Latham, T E; Wilson, F F; Çuhadar-Dönszelmann, T; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Thiessen, D; Khan, A; Kyberd, P; Teodorescu, L; Blinov, A E; Blinov, V E; Druzhinin, V P; Golubev, V B; Ivanchenko, V N; Kravchenko, E A; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Yushkov, A N; Best, D; Bruinsma, M; Chao, M; Eschrich, I; Kirkby, D; Lankford, A J; Mandelkern, M A; Mommsen, R K; Röthel, W; Stoker, D P; Buchanan, C; Hartfiel, B L; Foulkes, S D; Gary, J W; Shen, B C; Wang, K; Del Re, D; Hadavand, H K; Hill, E J; MacFarlane, D B; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Dahmes, B; Long, O; Lu, A; Mazur, M A; Richman, J D; Verkerke, W; Beck, T W; Eisner, A M; Heusch, C A; Kroseberg, J; Lockman, W S; Nesom, G; Schalk, T; Schumm, B A; Seiden, A; Spradlin, P; Williams, D C; Wilson, M G; Albert, J; Chen, E; Dubois-Felsmann, G P; Dvoretskii, A; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Ryd, A; Samuel, A; Yang, S; Jayatilleke, S M; Mancinelli, G; Meadows, B T; Sokoloff, M D; Abe, T; Blanc, F; Bloom, P; Chen, S; Ford, W T; Nauenberg, U; Olivas, A; Rankin, P; Smith, J G; Zhang, J; Zhang, L; Chen, A; Harton, J L; Soffer, A; Toki, W H; Wilson, R J; Zeng, Q; Altenburg, D; Brandt, T; Brose, J; Dickopp, M; Feltresi, E; Hauke, A; Lacker, H M; Müller-Pfefferkorn, R; Nogowski, R; Otto, S; Petzold, A; Schubert, J; Schubert, Klaus R; Schwierz, R; Spaan, B; Sundermann, J E; Bernard, D; Bonneaud, G R; Brochard, F; Grenier, P; Schrenk, S; Thiebaux, C; Vasileiadis, G; Verderi, M; Bard, D J; Clark, P J; Lavin, D; Muheim, F; Playfer, S; Xie, Y; Andreotti, M; Azzolini, V; Bettoni, D; Bozzi, C; Calabrese, R; Cibinetto, G; Luppi, E; Negrini, M; Piemontese, L; Sarti, A; Treadwell, E; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Patteri, P; Peruzzi, I M; Piccolo, M; Zallo, A; Buzzo, A; Capra, R; Contri, R; Crosetti, G; Lo Vetere, M; Macri, M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Bailey, S; Brandenburg, G; Chaisanguanthum, K S; Morii, M; Won, E; Dubitzky, R S; Langenegger, U; Bhimji, W; Bowerman, D A; Dauncey, P D; Egede, U; Gaillard, J R; Morton, G W; Nash, J A; Nikolich, M B; Taylor, G P; Charles, M J; Grenier, G J; Mallik, U; Cochran, J; Crawley, H B; Lamsa, J; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Yi, J; Biasini, M; Covarelli, R; Pioppi, M; Davier, M; Giroux, X; Grosdidier, G; Höcker, A; Laplace, S; Le Diberder, F R; Lepeltier, V; Lutz, A M; Petersen, T C; Plaszczynski, S; Schune, M H; Tantot, L; Wormser, G; Cheng, C H; Lange, D J; Simani, M C; Wright, D M; Bevan, A J; Chavez, C A; Coleman, J P; Forster, I J; Fry, J R; Gabathuler, Erwin; Gamet, R; Hutchcroft, D E; Parry, R J; Payne, D J; Sloane, R J; Touramanis, C; Back, J J; Harrison, P F; Mohanty, G B; Cowan, G; Flack, R L; Flächer, H U; Green, M G; Jackson, P S; McMahon, T R; Ricciardi, S; Salvatore, F; Winter, M A; Brown, D; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Hart, P A; Hodgkinson, M C; Lafferty, G D; Lyon, A J; Williams, J C; Farbin, A; Hulsbergen, W D; Jawahery, A; Kovalskyi, D; Lae, C K; Lillard, V; Roberts, D A; Blaylock, G; Dallapiccola, C; Flood, K T; Hertzbach, S S; Kofler, R; Koptchev, V B; Moore, T B; Saremi, S; Stängle, H; Willocq, S; Cowan, R; Sciolla, G; Sekula, S J; Taylor, F; Yamamoto, R K; Mangeol, D J J; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L M; Eschenburg, V; Godang, R; Kroeger, R; Reidy, J; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Taras, P; Nicholson, H; Cavallo, N; Lista, L; Monorchio, D; Paolucci, P; Piccolo, D; Sciacca, C; Baak, M; Bulten, H; Raven, G; Snoek, H L; Wilden, L; Jessop, C P; LoSecco, J M; Allmendinger, T; Gan, K K; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Pulliam, T; Rahimi, A M; Ter-Antonian, R; Wong, Q K; Brau, J E; Frey, R; Igonkina, O; Potter, C T; Sinev, N B; Strom, D; Torrence, E; Colecchia, F; Dorigo, A; Galeazzi, F; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Tiozzo, G; Voci, C; Benayoun, M; Briand, H; Chauveau, J; David, P; La Vaissière, C de; Del Buono, L; Hamon, O; John, M J J; Leruste, P; Malcles, J; Ocariz, J; Pivk, M; Roos, L; T'Jampens, S; Therin, G; Manfredi, P F; Re, V; Behera, P K; Gladney, L; Guo, Q H; Panetta, J; Angelini, C; Batignani, G; Bettarini, S; Bondioli, M; Bucci, F; Calderini, G; Carpinelli, M; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Martínez-Vidal, F; Neri, N; Paoloni, E; Rama, M; Rizzo, G; Sandrelli, F; Walsh, J; Haire, M; Judd, D; Paick, K; Wagoner, D E; Danielson, N; Elmer, P; Lau, Y P; Lü, C; Miftakov, V; Olsen, J; Smith, A J S; Telnov, A V; Bellini, F; Cavoto, G; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Li Gioi, L; Mazzoni, M A; Morganti, S; Pierini, M; Piredda, G; Safai-Tehrani, F; Voena, C; Christ, S; Wagner, G; Waldi, R; Adye, T; De Groot, N; Franek, B J; Geddes, N I; Gopal, G P; Olaiya, E O; Aleksan, Roy; Emery, S; Gaidot, A; Ganzhur, S F; Giraud, P F; Hamel de Monchenault, G; Kozanecki, Witold; Legendre, M; London, G W; Mayer, B; Schott, G; Vasseur, G; Yéche, C; Zito, M; Purohit, M V; Weidemann, A W; Wilson, J R; Yumiceva, F X; Aston, D; Bartoldus, R; Berger, N; Boyarski, A M; Buchmüller, O L; Claus, R; Convery, M R; Cristinziani, M; De Nardo, Gallieno; Dong, D; Dorfan, J; Dujmic, D; Dunwoodie, W M; Elsen, E E; Fan, S; Field, R C; Glanzman, T; Gowdy, S J; Hadig, T; Halyo, V; Hast, C; Hrynóva, T; Innes, W R; Kelsey, M H; Kim, P; Kocian, M L; Leith, D W G S; Libby, J; Luitz, S; Lüth, V; Lynch, H L; Marsiske, H; Messner, R; Müller, D R; O'Grady, C P; Ozcan, V E; Perazzo, A; Perl, M; Petrak, S; Ratcliff, B N; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Simi, G; Snyder, A; Soha, A; Stelzer, J; Su, D; Sullivan, M K; Vavra, J; Wagner, S R; Weaver, M; Weinstein, A J R; Wisniewski, W J; Wittgen, M; Wright, D H; Yarritu, A K; Young, C C; Burchat, Patricia R; Edwards, A J; Meyer, T I; Petersen, B A; Roat, C; Ahmed, S; Alam, M S; Ernst, J A; Saeed, M A; Saleem, M; Wappler, F R; Bugg, W; Krishnamurthy, M; Spanier, S M; Eckmann, R; Kim, H; Ritchie, J L; Satpathy, A; Schwitters, R F; Izen, J M; Kitayama, I; Lou, X C; Ye, S; Bianchi, F; Bóna, M; Gallo, F; Gamba, D; Bosisio, L; Cartaro, C; Cossutti, F; Della Ricca, G; Dittongo, S; Grancagnolo, S; Lanceri, L; Poropat, P; Vuagnin, G; Panvini, R S; Banerjee, Sw; Brown, C M; Fortin, D; Jackson, P D; Kowalewski, R V; Roney, J M; Sobie, R J; Band, H R; Cheng, B; Dasu, S; Datta, M; Eichenbaum, A M; Graham, M; Hollar, J J; Johnson, J R; Kutter, P E; Li, H; Liu, R; Mihályi, A; Mohapatra, A K; Pan, Y; Prepost, R; Tan, P; Von Wimmersperg-Töller, J H; Wu, J; Wu, S L; Yu, Z; Greene, M G; Neal, H

    2004-01-01

    We present a study of the decays B0bar --> D(*)0 K(*)0bar using a sample of 124 million Upsilon(4S) --> B0bar B0 decays collected with the BaBar detector at the PEP-II asymmetric-energy e+e- collider at SLAC. We report evidence for the decay of B0 and B0bar mesons to the D*0 K_S final state with an average branching fraction BF(B --> D*0 K0bar) = (4.5 +/- 1.9 +/- 0.5) x 10^-5. Similarly, we measure BF(B --> D0 K0bar) = (6.2 +/- 1.2 +/- 0.4) x 10^-5 for the D0 K_S final state. We also measure BF(B0bar --> D0 K*0bar) = (6.2 +/- 1.4 +/- 0.6) x 10^-5 and set a 90% C.L. upper limit BF(B0bar --> D0bar K*0bar) < 4.1 x 10^-5. All results presented in this paper are preliminary.

  16. Bar pattern speeds in CALIFA galaxies. I. Fast bars across the Hubble sequence

    NARCIS (Netherlands)

    Aguerri, J. A. L.; Méndez-Abreu, J.; Falcón-Barroso, J.; Amorin, A.; Barrera-Ballesteros, J.; Cid Fernandes, R.; García-Benito, R.; García-Lorenzo, B.; González Delgado, R. M.; Husemann, B.; Kalinova, V.; Lyubenova, M.; Marino, R. A.; Márquez, I.; Mast, D.; Pérez, E.; Sánchez, S. F.; van de Ven, G.; Walcher, C. J.; Backsmann, N.; Cortijo-Ferrero, C.; Bland-Hawthorn, J.; del Olmo, A.; Iglesias-Páramo, J.; Pérez, I.; Sánchez-Blázquez, P.; Wisotzki, L.; Ziegler, B.

    2015-01-01

    Context. The bar pattern speed (Ωb) is defined as the rotational frequency of the bar, and it determines the bar dynamics. Several methods have been proposed for measuring Ωb. The non-parametric method proposed by Tremaine & Weinberg (1984, ApJ, 282, L5; TW) and based on stellar kinematics is the mo

  17. Bar Study Stories. Issues in Prevention

    Science.gov (United States)

    Higher Education Center for Alcohol, Drug Abuse, and Violence Prevention, 2012

    2012-01-01

    This issue of "Issues in Prevention" focuses on the impact of the availability of drinks in licensed establishments, such as bars and taverns on student drinking. This issue contains the following articles: (1) Cheap Drinks at College Bars Can Escalate Student Drinking (John D. Clapp); (2) High Alcohol Outlet Density: A Problem for Campuses and…

  18. Stabilizer bars: Part 3. Production methods

    Directory of Open Access Journals (Sweden)

    Adam-Markus WITTEK

    2011-01-01

    Full Text Available The article outlines the methods of stabilizer bar production as well as selected issues concerning the theory of cold roll forming. A proper construction and the selection of parameters are fundamental factors influencing the selection of a suitable production process. The selection of the manufacturing process has a fundamental impact on the quality and durability of the stabilizer bars.

  19. Stabilizer bars: Part 3. Production methods

    OpenAIRE

    Adam-Markus WITTEK; Hans-Christian RICHTER; Bogusław ŁAZARZ

    2011-01-01

    The article outlines the methods of stabilizer bar production as well as selected issues concerning the theory of cold roll forming. A proper construction and the selection of parameters are fundamental factors influencing the selection of a suitable production process. The selection of the manufacturing process has a fundamental impact on the quality and durability of the stabilizer bars.

  20. Adventures of a tidally induced bar

    CERN Document Server

    Lokas, E L; Debattista, V P; Valluri, M; del Pino, A; Semczuk, M; Gajda, G; Kowalczyk, K

    2014-01-01

    Using N-body simulations, we study the properties of a bar induced in a dwarf galaxy as a result of tidal interaction with the Milky Way. The dwarf is initially composed of a disk embedded in a dark matter halo and we follow its evolution on a typical orbit for 10 Gyr. It undergoes an evolution typical of tidally stirred dwarf galaxies: it loses mass, the stellar component transforms from a disk to a spheroid and the rotation of the stars is partially replaced by random motions. A tidally induced bar forms at the first pericentre passage and survives until the end of the evolution. Fourier decomposition of the distribution of stars reveals that only even modes are significant and preserve a hierarchy so that the bar mode is always the strongest. They show a characteristic profile with a maximum, similar to simulated bars forming in isolated galaxies and observed bars in real galaxies. We adopt the maximum of the bar mode as a measure of the bar strength and we estimate the bar length by comparing the density ...

  1. Constraints from microlensing on the COBE bar

    Science.gov (United States)

    Zhao, H. S.

    Since the first review of converging evidences for a bar in the center of the Galaxy by de Zeeuw (1992) at the IAU Sym. 153 in Gent five years ago, the Galactic bar idea has been put on a solid footing by an influx of new data (COBE/DIRBE maps, star count data of bulge red clump giants, microlensing optical depth, and bulge stellar proper motions, etc.) and a burst of increasingly sophisticated theoretical models (triaxial luminosity models of Dwek et al. 1994, and Binney, Gerhard & Spergel 1997, steady state stellar bar dynamical model of Zhao 1996, combined luminosity, microlensing and gas kinematics models of Zhao, Rich & Spergel 1996, and Bissantz et al. 1997, etc.), which fit new data and improve upon earlier simple bulge/bar models (Kent 1992, Binney et al. 1991, Blitz & Spergel 1991). While research in this field shifts more and more to constraining the exact phase space and parameter space of the bar, both the non-uniqueness of and the mismatches among bars from different datasets start to show up. I compare the bar from microlensing data with the COBE bar and point out the effects the non-uniqueness.

  2. Tubular stabilizer bars – calculations and construction

    Directory of Open Access Journals (Sweden)

    Adam-Markus WITTEK

    2011-01-01

    Full Text Available The article outlines the calculation methods for tubular stabilizer bars. Modern technological and structural solutions in contemporary cars are reflected also in the construction, selection and manufacturing of tubular stabilizer bars. A proper construction and the selection of parameters influence the strength properties, the weight, durability and reliability as well as the selection of an appropriate production method.

  3. Stabilizer bars: Part 1. Calculations and construction

    Directory of Open Access Journals (Sweden)

    Adam-Markus WITTEK

    2010-01-01

    Full Text Available The article outlines the calculation methods for stabilizer bars. Modern technological and structural solutions in contemporary cars are reflected also in the construction and manufacturing of stabilizer bars. A proper construction and the selection of parameters influence the strength properties, the weight, durability and reliability as well as the selection of an appropriate production method.

  4. Herboxidiene triggers splicing repression and abiotic stress responses in plants

    KAUST Repository

    Alshareef, Sahar

    2017-03-27

    Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.

  5. A molecular inversion probe assay for detecting alternative splicing

    Directory of Open Access Journals (Sweden)

    Palm Curtis

    2010-12-01

    Full Text Available Absract Background A sensitive, high-throughput method for monitoring pre-mRNA splicing on a genomic scale is needed to understand the spectrum of alternatively spliced mRNA in human cells. Results We adapted Molecular Inversion Probes (MIPs, a padlock-probe based technology, for the multiplexed capture and quantitation of individual splice events in human tissues. Individual MIP capture probes can be quantified using either DNA microarrays or high-throughput sequencing, which permits independent assessment of each spliced junction. Using our methodology we successfully identified 100% of our positive controls and showed that there is a strong correlation between the data from our alternative splicing MIP (asMIP assay and quantitative PCR. Conclusion The asMIP assay provides a sensitive, accurate and multiplexed means for measuring pre-mRNA splicing. Fully optimized, we estimate that the assay could accommodate a throughput of greater than 20,000 splice junctions in a single reaction. This would represent a significant improvement over existing technologies.

  6. Inference of splicing regulatory activities by sequence neighborhood analysis.

    Directory of Open Access Journals (Sweden)

    Michael B Stadler

    2006-11-01

    Full Text Available Sequence-specific recognition of nucleic-acid motifs is critical to many cellular processes. We have developed a new and general method called Neighborhood Inference (NI that predicts sequences with activity in regulating a biochemical process based on the local density of known sites in sequence space. Applied to the problem of RNA splicing regulation, NI was used to predict hundreds of new exonic splicing enhancer (ESE and silencer (ESS hexanucleotides from known human ESEs and ESSs. These predictions were supported by cross-validation analysis, by analysis of published splicing regulatory activity data, by sequence-conservation analysis, and by measurement of the splicing regulatory activity of 24 novel predicted ESEs, ESSs, and neutral sequences using an in vivo splicing reporter assay. These results demonstrate the ability of NI to accurately predict splicing regulatory activity and show that the scope of exonic splicing regulatory elements is substantially larger than previously anticipated. Analysis of orthologous exons in four mammals showed that the NI score of ESEs, a measure of function, is much more highly conserved above background than ESE primary sequence. This observation indicates a high degree of selection for ESE activity in mammalian exons, with surprisingly frequent interchangeability between ESE sequences.

  7. Global Splicing Pattern Reversion during Somatic Cell Reprogramming

    Directory of Open Access Journals (Sweden)

    Sho Ohta

    2013-10-01

    Full Text Available Alternative splicing generates multiple transcripts from a single gene, and cell-type-specific splicing profiles are important for the properties and functions of the cells. Recently, somatic cells have been shown to undergo dedifferentiation after the forced expression of transcription factors. However, it remains unclear whether somatic cell splicing is reorganized during reprogramming. Here, by combining deep sequencing with high-throughput absolute qRT-PCR, we show that somatic splicing profiles revert to pluripotent ones during reprogramming. Remarkably, the splicing pattern in pluripotent stem cells resembles that in testes, and the regulatory regions have specific characteristics in length and sequence. Furthermore, our siRNA screen has identified RNA-binding proteins that regulate splicing events in iPSCs. We have then demonstrated that two of the RNA-binding proteins, U2af1 and Srsf3, play a role in somatic cell reprogramming. Our results indicate that the drastic alteration in splicing represents part of the molecular network involved in the reprogramming process.

  8. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Third Results Reports

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2012-05-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. NREL has previously published two reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from July 2011 through January 2012.

  9. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Fourth Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2013-01-01

    SunLine Transit Agency, which provides public transit services to the Coachella Valley area of California, has demonstrated hydrogen and fuel cell bus technologies for more than 10 years. In May 2010, SunLine began demonstrating the advanced technology (AT) fuel cell bus with a hybrid electric propulsion system, fuel cell power system, and lithium-based hybrid batteries. This report describes operations at SunLine for the AT fuel cell bus and five compressed natural gas buses. The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) is working with SunLine to evaluate the bus in real-world service to document the results and help determine the progress toward technology readiness. NREL has previously published three reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from February 2012 through November 2012.

  10. Design and VHDL simulation of a special high speed spaceborne bus controller

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The application of high speed data bus on the satellites having several remote sensors could not only satisfy the demand of high speed data transfer,but also could reuse the equipment at both the com-ponent and subsystem levels for other space missions,thus reducing the costs of on-board processing system integration.In this article,a special high speed data bus with simple interfaces is chosen for transferring the data produced by remote sensors and other instrumentations.First,the function and the structure of the data bus are discussed carefully.And then,the bus controller,which is a key mod-ule of the data bus,is designed.At last,the function and performance of the bus controller are verified by VHDL simulation.

  11. Bitmap-Wise Wireless M-Bus Coordination for Sustainable Real Time Energy Management

    Directory of Open Access Journals (Sweden)

    Kwang-il Hwang

    2014-07-01

    Full Text Available Even though WM-Bus is being considered to be the most promising network protocol for smart metering, it is not suitable for a sustainable real-time home energy management system (HEMS, which requires higher reliability and longer lifetime despite real time bi-directional communications. Therefore, in this paper we propose a Bitmap-wise WM-Bus (BWM-Bus, coping well with sustainable real-time HEMS. In particular, the proposed scheme addresses the several problems in WM-Bus for HEMS by introducing novel functions: asynchronous meter trigger, adaptive slot scheduling, and bitmap-wise retransmission request. Through experiments, we demonstrate that BWM-Bus guarantees higher data success ratio with lower data aggregation time, as well as longer lifetime than WM-Bus standard.

  12. Characterization of Bars Induced by Interactions

    Directory of Open Access Journals (Sweden)

    Inma Martinez-Valpuesta

    2016-05-01

    Full Text Available Whether the formation of bars is triggered by interactions or by internal processes has been discussed for many decades. In this work, we study differences between both mechanisms by means of numerical simulations. We relate our analysis to fly-by interactions in different mass groups or clusters according to the velocity of the encounters. We find that once the bar is created, the interaction does not much affect its evolution. We also find that bars can be triggered purely by a slow interaction. Those bars affected or triggered by interaction stay for a longer time in the slow regime, i.e., the corotation radius is more than 1.4 times the bar radius.

  13. Dynamical evolution of two associated galactic bars

    CERN Document Server

    Garzon, F

    2014-01-01

    We study the dynamical interactions of mass systems in equilibrium under their own gravity that mutually exert and experience gravitational forces. The method we employ is to model the dynamical evolution of two isolated bars, hosted within the same galactic system, under their mutual gravitational interaction. In this study we present an analytical treatment of the secular evolution of two bars that oscillate with respect one another. Two cases of interaction, with and without geometrical deformation, are discussed. In the latter case, the bars are described as modified Jacobi ellipsoids. These triaxial systems are formed by a rotating fluid mass in gravitational equilibrium with its own rotational velocity and the gravitational field of the other bar. The governing equation for the variation of their relative angular separation is then numerically integrated, which also provides the time evolution of the geometrical parameters of the bodies. The case of rigid, non-deformable, bars produces in some cases an ...

  14. [Development of cereal bar with pineapple skin].

    Science.gov (United States)

    Fonseca, Renata Siqueira; Del Santo, Victor Rogério; Souza, Gilberto Batista de; Pereira, Cíntia Alessandra Matiucci

    2011-06-01

    The cereal bars are multi-component products consisting of cereals, dried fruit and syrup binder and may be added to the consumable parts of fruits and vegetables which usually are not exploited and have high nutritional value, thereby reducing food waste. It was developed a jam with pineapple skin, which it was utilized in 13.5% in the cereal bar formulation. The cereal bar was sensorial evaluated and had its centesimal and mineral composition determined. The new product achieved average of 8.3 for global impression using 9 points hedonic scale, 91% of acceptance rate and 67% of purchase intent. In this first use of pineapple skin jam as food ingredient it can be concluded that its aggregation in the cereal bar formula is feasible, making an accepted product with fibers, proteins and minerals, as an alternative to traditional cereal bars.

  15. A COMPLETE THREE-DIMENSIONAL FINITE ELEMENT ANALYSIS OF THE BAR-BAR TENSILE IMPACT APPARATUS

    Institute of Scientific and Technical Information of China (English)

    万华培; 汪洋; 夏源明

    2003-01-01

    A complete three-dimensional FEM model of the Bar-Bar Tensile Impact Apparatus (BTIA) is constructed, in which the slots in the bars and the glue layers between the bars and the flat-shaped specimen are included. For elastic-plastic specimen material, Ly12cz aluminum alloy, the process of tensile impact experiments is simulated and the matching relation between the specimen geometry and the bars is investigated. Based on the FEM analysis, an iterative method is proposed to design a reasonable specimen geometry for obtaining the true dynamic stress-strain relation for a certain specimen material.

  16. The influence of bus stop on traffic flow with velocity-difference-separation model

    Science.gov (United States)

    Zheng, Pengjun; Wang, Wei; Ge, Hongxia

    2016-06-01

    Based on velocity-difference-separation model, the mixed traffic flow on two-lane road is investigated. For a fixed road length, the influence of bus and bus stops on traffic flow is studied with the increasing traffic density. Compared with the result without bus stops given by Li et al., a new traffic state is found, which is valuable for studying the impacts of public transport on urban traffic flow.

  17. Introduction to corridor selection & assessment for Bus Rapid Transit System (BRTS) in Hyderabad

    OpenAIRE

    Bhanu Kireeti Chanda; Maddali Sai Satya Goutham

    2014-01-01

    Bus rapid transit system is an innovation and it is up gradation to the present transport system. BRT is affordable, adaptable and cost effective. The term of rapid transit system means a form of high speed urban passenger transportation. Here, the mode of transportation is bus hence it is called as bus rapid transit system (BRTS). Transportation facilities and the system will be different form different countries. Especially for the developing countries, transportation is a problem and BR...

  18. Small Explorer Data System MIL-STD-1773 fiber optic bus

    Science.gov (United States)

    Flanegan, Mark; Label, Ken

    1992-06-01

    The MIL-STD-1773 Fiber Optic Data Bus as implemented in the GSFC Small Explorer Data System (SEDS) for the Small Explorer Program is described. It provides an overview of the SEDS MIL-STD-1773 bus components system design considerations, reliability figures, acceptance and qualification testing requirements, radiation requirements and tests, error handling considerations, and component heritage. The first mission using the bus will be launched in June of 1992.

  19. Alternative splicing variations in mouse CAPS2: differential expression and functional properties of splicing variants

    Directory of Open Access Journals (Sweden)

    Furuichi Teiichi

    2007-04-01

    Full Text Available Abstract Background Ca2+-dependent activator protein 2 (CAPS2/CADPS2 is a secretory vesicle-associated protein involved in the release of neurotrophin. We recently reported that an aberrant, alternatively spliced CAPS2 mRNA that lacks exon 3 (CAPS2Δexon3 is detected in some patients with autism. Splicing variations in mouse CAPS2 and their expression and functions remain unclear. Results In this study, we defined 31 exons in the mouse CAPS2 gene and identified six alternative splicing variants, CAPS2a-f. CAPS2a is an isoform lacking exons 22 and 25, which encode part of the Munc13-1-homologous domain (MHD. CAPS2b lacks exon 25. CAPS2c lacks exons 11 and 22. CAPS2d, 2e, and 2f have C-terminal deletions from exon 14, exon 12, and exon 5, respectively. On the other hand, a mouse counterpart of CAPS2Δexon3 was not detected in the mouse tissues tested. CAPS2b was expressed exclusively in the brain, and the other isoforms were highly expressed in the brain, but also in some non-neural tissues. In the brain, all isoforms showed predominant expression patterns in the cerebellum. In the developing cerebellum, CAPS2b showed an up-regulated expression pattern, whereas the other isoforms exhibited transiently peaked expression patterns. CAPS2 proteins were mostly recovered in soluble fractions, but some were present in membrane fractions, except for CAPS2c and 2f, both of which lack the PH domain, suggesting that the PH domain is important for membrane association. In contrast to CAPS2a and 2b, CAPS2c showed slightly decreased BDNF-releasing activity, which is likely due to the C-terminal truncation of the PH domain in CAPS2c. Conclusion This study indicates that, in mouse, there are six splicing variants of CAPS2 (CAPS2a-f, and that these are subdivided into two groups: a long form containing the C-terminal MHD and a short form lacking the C-terminal MHD. These results demonstrate that the splicing variations correlate with their expression patterns and

  20. A Prediction System Using a P2P Overlay Network for a Bus Arrival System

    Directory of Open Access Journals (Sweden)

    Ssu-Hsuan Lu

    2014-01-01

    Full Text Available Along with the evolution of times and the surge of metropolitan populations, government agencies often promote the construction of public transport. Unlike rail transportation or rapid transit systems, it is often difficult to estimate the vehicle arrival times at each station in a bus transportation system due to metropolitan transportation congestion. Traffic status is often monitored using wireless sensor networks (WSNs. However, WSNs are always separated from one another spatially. Recent studies have considered the connection of multiple sensor networks. This study considers a combination view of peer-to-peer (P2P overlay networks and WSN architecture to predict bus arrival times. Each bus station, which is also a P2P overlay peer, is connected in a P2P overlay network. A sensor installed in each bus can receive data via peers to obtain the moving speed of a bus. Then, each peer can exchange its data to predict bus arrival times at bus stations. This method can considerably increase the accuracy with which bus arrival times can be predicted and can provide traffic status with high precision. Furthermore, these data can also be used to plan new bus routes according to the information gathered.

  1. An interactive wireless communication system for visually impaired people using city bus transport.

    Science.gov (United States)

    Wang, Hsiao-Lan; Chen, Ya-Ping; Rau, Chi-Lun; Yu, Chung-Huang

    2014-04-25

    Visually impaired people have difficulty accessing information about public transportation systems. Several systems have been developed for assisting visually impaired and blind people to use the city bus. Most systems provide only one-way communication and require high-cost and complex equipment. The purpose of this study is to reduce the difficulties faced by visually impaired people when taking city buses, using an interactive wireless communication system. The system comprised a user module and a bus module to establish a direct one-to-one connection. When the user inputs 4-digit numbers, the user module immediately sends out the information. If the bus module receives the matched bus number, it buzzes and the warning LED flashes to notify the bus driver that someone is waiting to board on the bus. User tests were conducted by two visually impaired people in a simulated vehicle and a city bus. The success rate of interactive wireless communication, recognizing the arrival of the bus and boarding the correct bus reached 100% in all of the tests. The interactive wireless communication aid system is a valid and low-cost device for assisting visually impaired people to use city buses.

  2. Do Bars Drive Spiral Density Waves?

    CERN Document Server

    Buta, R J; Elmegreen, B G; Salo, H; Laurikainen, E; Elmegreen, D M; Puerari, I; Block, D L

    2009-01-01

    We present deep near-infrared K_s-band AAT IRIS2 observations of a selected sample of nearby barred spiral galaxies, including some with the strongest known bars. The sample covers a range of Hubble types from SB0- to SBc. The goal is to determine if the torque strengths of the spirals correlate with those of the bars, which might be expected if the bars actually drive the spirals as has been predicted by theoretical studies. This issue has implications for interpreting bar and spiral fractions at high redshift. Analysis of previous samples suggested that such a correlation exists in the near-infrared, where effects of extinction and star formation are less important. However, the earlier samples had only a few excessively strong bars. Our new sample largely confirms our previous studies, but still any correlation is relatively weak. We find two galaxies, NGC 7513 and UGC 10862, where there is a only a weak spiral in the presence of a very strong bar. We suggest that some spirals probably are driven by their ...

  3. Physicochemical Evaluation of Flaxseed-Date Bar

    Directory of Open Access Journals (Sweden)

    Maimuna Sultana Mohd. Ateequddin

    2015-09-01

    Full Text Available The objective of this study was to develop a flaxseed-date-bar fortified high level of dietary fibre by using functional ingredients. Formulations were developed containing 0, 5, 10, 15 and 20 grams of flaxseed flour. Flaxseed- date bar were evaluated for their, Physic-chemical properties. In the physical evaluation of bar it was found that flaxseed flour increase the hardness from 1076.82g to 1403.12g.The results revealed that the bar containing nuts and oilseeds shows maximum protein content (10.09 percent, dietary fibre content (9.89 percent, and ash content(2.90 percent was found in flaxseed-date bar. The gross energy of the flaxseed-date bar was in the range of 353.75 kcal to 377.08 kcal .The maximum gross energy was recorded by following sample A4were as the minimum was obtained by A0. The result revealed that flaxseed-dates along with nuts can be useful to prepare flaxseed-date bars of good sensory and nutritional value which provide substantial amount of carbohydrates, proteins, fats and dietary fibre.

  4. Tidally Induced Bars of Galaxies in Clusters

    Science.gov (United States)

    Łokas, Ewa L.; Ebrová, Ivana; del Pino, Andrés; Sybilska, Agnieszka; Athanassoula, E.; Semczuk, Marcin; Gajda, Grzegorz; Fouquet, Sylvain

    2016-08-01

    Using N-body simulations, we study the formation and evolution of tidally induced bars in disky galaxies in clusters. Our progenitor is a massive, late-type galaxy similar to the Milky Way, composed of an exponential disk and a Navarro-Frenk-White dark matter halo. We place the galaxy on four different orbits in a Virgo-like cluster and evolve it for 10 Gyr. As a reference case, we also evolve the same model in isolation. Tidally induced bars form on all orbits soon after the first pericenter passage and survive until the end of the evolution. They appear earlier, are stronger and longer, and have lower pattern speeds for tighter orbits. Only for the tightest orbit are the properties of the bar controlled by the orientation of the tidal torque from the cluster at pericenter. The mechanism behind the formation of the bars is the angular momentum transfer from the galaxy stellar component to its halo. All of the bars undergo extended periods of buckling instability that occur earlier and lead to more pronounced boxy/peanut shapes when the tidal forces are stronger. Using all simulation outputs of galaxies at different evolutionary stages, we construct a toy model of the galaxy population in the cluster and measure the average bar strength and bar fraction as a function of clustercentric radius. Both are found to be mildly decreasing functions of radius. We conclude that tidal forces can trigger bar formation in cluster cores, but not in the outskirts, and thus can cause larger concentrations of barred galaxies toward the cluster center.

  5. Binding of a candidate splice regulator to a calcitonin-specific splice enhancer regulates calcitonin/CGRP pre-mRNA splicing.

    Science.gov (United States)

    Coleman, Timothy P; Tran, Quincy; Roesser, James R

    2003-01-27

    The calcitonin/calcitonin gene-related peptide (CGRP) pre-mRNA is alternatively processed in a tissue-specific manner leading to the production of calcitonin mRNA in thyroid C cells and CGRP mRNA in neurons. A candidate calcitonin/CGRP splice regulator (CSR) isolated from rat brain was shown to inhibit calcitonin-specific splicing in vitro. CSR specifically binds to two regions in the calcitonin-specific exon 4 RNA previously demonstrated to function as a bipartate exonic splice enhancer (ESE). The two regions, A and B element, are necessary for inclusion of exon 4 into calcitonin mRNA. A novel RNA footprinting method based on the UV cross-linking assay was used to define the site of interaction between CSR and B element RNA. Base changes at the CSR binding site prevented CSR binding to B element RNA and CSR was unable to inhibit in vitro splicing of pre-mRNAs containing the mutated CSR binding site. When expressed in cells that normally produce predominantly CGRP mRNA, a calcitonin/CGRP gene containing the mutated CSR binding site expressed predominantly calcitonin mRNA. These observations demonstrate that CSR binding to the calcitonin-specific ESE regulates calcitonin/CGRP pre-mRNA splicing.

  6. Designing oligo libraries taking alternative splicing into account

    Science.gov (United States)

    Shoshan, Avi; Grebinskiy, Vladimir; Magen, Avner; Scolnicov, Ariel; Fink, Eyal; Lehavi, David; Wasserman, Alon

    2001-06-01

    We have designed sequences for DNA microarrays and oligo libraries, taking alternative splicing into account. Alternative splicing is a common phenomenon, occurring in more than 25% of the human genes. In many cases, different splice variants have different functions, are expressed in different tissues or may indicate different stages of disease. When designing sequences for DNA microarrays or oligo libraries, it is very important to take into account the sequence information of all the mRNA transcripts. Therefore, when a gene has more than one transcript (as a result of alternative splicing, alternative promoter sites or alternative poly-adenylation sites), it is very important to take all of them into account in the design. We have used the LEADS transcriptome prediction system to cluster and assemble the human sequences in GenBank and design optimal oligonucleotides for all the human genes with a known mRNA sequence based on the LEADS predictions.

  7. Alternative splicing of follicle-stimulating hormone receptor pre-mRNA: cloning and characterization of two alternatively spliced mRNA transcripts

    NARCIS (Netherlands)

    R. Kraaij (Robert); M. Verhoef-Post (Miriam); J.A. Grootegoed (Anton); A.P.N. Themmen (Axel)

    1998-01-01

    textabstractGlycoprotein hormone receptors contain a large extracellular domain that is encoded by multiple exons, facilitating the possibility of expressing alternatively spliced transcripts. We have cloned two new splice variants of the rat follicle-stimulating hormon

  8. First Measurement of σ(gg → t$\\bar{t}$)/σ(p$\\bar{p}$ → t$\\bar{t}$)

    Energy Technology Data Exchange (ETDEWEB)

    Alamdari, Shabnaz Pashapour [Univ. of Toronto, ON (Canada)

    2008-01-01

    The work presented here is the first measurement of the fraction of top quark pair production through gluon-gluon fusion. We use an integrated luminosity of 0.96 ± 0.06 fb-1 of p{bar p} collisions at √s of 1.96 TeV collected by the CDF II detector. We select t$\\bar{t}$ candidates by identifying a high-pT lepton candidate, a large missing ET as evidence for a neutrino candidate and at least four high ET jets, one of which has to be identified as originating from a b quark. The challenge is to discriminate between the two production processes with the identical final state, gg → t$\\bar{t}$ and q$\\bar{p}$ → t$\\bar{t}$. We take advantage of the fact that compared to a quark, a gluon is more likely to radiate a low momentum gluon and therefore, one expects a larger number of charged particles with low pT in a process involving more gluons. Given the large uncertainties associated with the modeling of the low pT charged particle multiplicity, a data-driven technique was employed. Using calibration data samples, we show there exists a clear correlation between the observed average number of low pT charged particles and the average number of gluons involved in the production process predicted by Monte Carlo calculations. Given the correlation, one can identify low pT charged particle multiplicity distributions associated with specific average number of gluons. The W + 0 jet sample and dijets sample with leading jet ET in the range of 80-100 GeV are used to find no-gluon and gluon-rich low p{sub T} charged particle multiplicity distributions, respectively. Using these no-gluon and gluon-rich distributions in a likelihood fit, we find the fraction of gluon-rich events in t{bar t} candidates. This fraction has contributions from the signal and background events. Taking into account these contributions and the gg → t$\\bar{t}$ and q$\\bar{q}$ → t$\\bar

  9. Communication Architecture Synthesis for Multi-bus SoC

    Directory of Open Access Journals (Sweden)

    Abdelkrim Zitouni

    2006-01-01

    Full Text Available In the systems on chip (SoC design, the synthesis of communication architecture constitutes the bottleneck which can affect the performances of the system. Various schemes and protocols can be necessary, just as various topologies of interconnection. To reduce the complexity of the communications refinement, we present in this study a model and a synthesis approach for multi-bus communication architecture containing centralized bridge. The automation of the arbiter synthesis step profited from a detailed attention. This stage generates a hierarchical arbiter integrating various priority arbitration modules. The proposed approach was integrated in a toolbox based environment

  10. Fuel options for public bus fleets in Sweden

    OpenAIRE

    Xylia, Maria; Silveira, Semida

    2015-01-01

    The Swedish public transport sector has defined two major targets, i.e., to run 90% of the total vehicle kilometers of the fleet on non-fossil fuels and double the volume of travel via public transport by 2020, increasing the share of public transport in relation to the total personal transport in the country . The f3 report Fuel options for public bus fleets in Sweden highlights the challenges and solutions encountered, particularly when it comes to the adoption of renewable fuels in the reg...

  11. BC Transit Fuel Cell Bus Project Evaluation Results: Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Post, M.

    2014-09-01

    Second report evaluating a fuel cell electric bus (FCEB) demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. NREL published its first report on the demonstration in February 2014. This report is an update to the previous report; it covers 3 full years of revenue service data on the buses from April 2011 through March 2014 and focuses on the final experiences and lessons learned.

  12. Development of a Multi‐bus platform for automation testbed

    OpenAIRE

    Isaksson, Mathias; Knapik, Lukas

    2010-01-01

    The task for this thesis was to develop, construct and evaluate a multi‐bus communication system,connected to a PC via USB and capable of communicating in CAN, I2C and SPI and develop drivers for itin National Instruments LabVIEW.In the beginning a study was made of the communication buses followed by an investigation of whattype of hardware that could accomplish this task. A microcontroller unit was selected andprogrammed in MikroElektronika MikroC Pro v.3.2 to act as the interface between t...

  13. Study on Braking Energy Regeneration System for City Bus

    Institute of Scientific and Technical Information of China (English)

    ZOU Zheng-yao; CHEN Ru-wen; YANG Xue-mei; LIU Gou-bing; JIN Jia-jun

    2011-01-01

    Braking of the urban vehicles of public service wastes a large number of engine energy in the condition of starting and stopping frequently. Aiming at the problem, an electro-mechanical braking energy regeneration system was proposed which adopted a high-speed flywheel and a battery to recover the braking energy and achieve the secondary traction for the auxiliary start function. The system strategy was designed and the braking simulation was processed to validate its feasibility. The experiment results show that the system can effectively recover the braking energy, improve the starting performance of the city bus and it can be applied to the engineering.

  14. Distributed Data Acquisition and Control by Software Bus

    Institute of Scientific and Technical Information of China (English)

    Cecil Bruce-Boye; Dmitry A. Kazakov

    2004-01-01

    Increasing global competition forces manufacturers of products from all technical fields to guarantee a high product quality for a long period of time. At the same time it is necessary to minimize production costs. In order to meet all these requirements, on-line data acquisition and processing are of increasing importance in distributed automation systems. A software bus operating on industrial Ethernet has an ability to minimize operating costs by offering easy installation, scalability,high degree of reliability and remote monitoring and control.

  15. Bus ride: an ethnographic exercise in Cafezal Quarter

    OpenAIRE

    Fé, Janine Damasceno Moura; Universidade Federal do Piauí; Souza, Maria Irene Pellegrino de Oliveira; Universidade Estadual de Londrina

    2006-01-01

     This work studies ethnography with theory basis from Collier Jr; Achutti; Maffesoli. The research method is photography and the target population are workers and passengers of a bus line, the Cafezal (quarter in the outskirts of Londrina-PR). It is noted that even in the most regular situations, it is possible to capture significant elements for ethnographic research.   O trabalho estuda a etnografia com referencial teórico de Collier Jr; Achutti, Maffesoli. O método de pesquisa é a fotog...

  16. DC Bus Regulation with a Flywheel Energy Storage System

    Science.gov (United States)

    Kenny, Barbara H.; Kascak, Peter E.

    2003-01-01

    This paper describes the DC bus regulation control algorithm for the NASA flywheel energy storage system during charge, charge reduction and discharge modes of operation. The algorithm was experimentally verified with results given in a previous paper. This paper presents the necessary models for simulation with detailed block diagrams of the controller algorithm. It is shown that the flywheel system and the controller can be modeled in three levels of detail depending on the type of analysis required. The three models are explained and then compared using simulation results.

  17. The peculiarities of large intron splicing in animals.

    Science.gov (United States)

    Shepard, Samuel; McCreary, Mark; Fedorov, Alexei

    2009-11-16

    In mammals a considerable 92% of genes contain introns, with hundreds and hundreds of these introns reaching the incredible size of over 50,000 nucleotides. These "large introns" must be spliced out of the pre-mRNA in a timely fashion, which involves bringing together distant 5' and 3' acceptor and donor splice sites. In invertebrates, especially Drosophila, it has been shown that larger introns can be spliced efficiently through a process known as recursive splicing-a consecutive splicing from the 5'-end at a series of combined donor-acceptor splice sites called RP-sites. Using a computational analysis of the genomic sequences, we show that vertebrates lack the proper enrichment of RP-sites in their large introns, and, therefore, require some other method to aid splicing. We analyzed over 15,000 non-redundant, large introns from six mammals, 1,600 from chicken and zebrafish, and 560 non-redundant large introns from five invertebrates. Our bioinformatic investigation demonstrates that, unlike the studied invertebrates, the studied vertebrate genomes contain consistently abundant amounts of direct and complementary strand interspersed repetitive elements (mainly SINEs and LINEs) that may form stems with each other in large introns. This examination showed that predicted stems are indeed abundant and stable in the large introns of mammals. We hypothesize that such stems with long loops within large introns allow intron splice sites to find each other more quickly by folding the intronic RNA upon itself at smaller intervals and, thus, reducing the distance between donor and acceptor sites.

  18. [Investigation on events of bus on fire in 6 years in the mainland of China].

    Science.gov (United States)

    Wang, X G; Liu, Y; Cen, Y; Wu, P; Zhou, H L; Han, C M

    2016-12-20

    Objective: To retrospectively analyze the characteristics of events of bus on fire in 6 years in the mainland of China. Methods: Events of bus on fire happened between January 2009 and December 2014 were retrieved through Baidu search engine, Chinese Journals Full-text Database, and PubMed database in the search strategy with " bus" and " fire" or " arson" as keywords combined with the name of provinces, autonomous regions, and municipalities of the mainland of China. The occurrence time, region, cause of fire, casualties of each event were recorded, and the correlative analysis was conducted. Data were processed with Microsoft Excel software. Results: Totally 287 events of bus on fire were retrieved, among which 49 events happened in 2009, 36 events happened in 2010, 35 events happened in 2011, 37 events happened in 2012, and respectively 65 events happened in 2013 and 2014. The events of bus on fire most frequently happened in June and July, respectively 49 and 39 events. Among the distribution of occurrence regions of events of bus on fire, there were 78 events (27.18%) in east China, 52 events (18.12%) in northeast China, 41 events (14.29%) both in north China and south China. Among the causes of events of bus on fire, spontaneous combustion of bus ranked in the first (267 events, accounting for 93.03%), followed by arson (13 events, accounting for 4.53%). Among the 13 events of bus on fire caused by arson, 7 events happened between 16: 00 and 20: 00, and 3 events happened between 8: 00 and 10: 00. Totally 27 events of bus on fire (9.41%) were with casualties, among which 13 events (48.15%) were caused by spontaneous combustion of bus, 10 events (37.04%) were caused by arson, and 4 events (14.81%) were caused by traffic accidents. Arson caused the most severe casualties (at least 88 deaths and 287 injuries), followed by spontaneous combustion of bus (at least 35 deaths and 140 injuries) and traffic accidents (at least 9 deaths and 20 injuries). Conclusions

  19. Possible $D\\bar{D}$ and $B\\bar{B}$ Molecular states in a chiral quark model

    CERN Document Server

    Li, M T; Dong, Y B; Zhang, Z Y

    2012-01-01

    We perform a systematic study of the bound state problem of $D\\bar{D}$ and $B\\bar{B}$ systems by using effective interaction in our chiral quark model. Our results show that both the interactions of $D\\bar{D}$ and $B\\bar{B}$ states are attractive, which consequently result in $I^G(J^{PC})=0^+(0^{++})$ $D\\bar{D}$ and $B\\bar{B}$ bound states.

  20. Arrangement to control the roof bar depending on a sliding bar. Einrichtung zur Steuerung einer Hangendkappe in Abhaengigkeit einer Schiebekappe

    Energy Technology Data Exchange (ETDEWEB)

    Weirich, W.; Dettmers, M.

    1984-04-12

    The invention is concerned with an arrangement to control an underground powered support unit the roof bar of which is furnished with a regulating bar. The regulating cylinder of the regulating bar is hydraulically coupled with the sliding cylinder of the sliding bar in such a way that on pulling of the sliding bar the pressing pressure of the roof bar to the roof is reduced automatically to a level which can be preset. (orig.).

  1. Desmin splice variants causing cardiac and skeletal myopathy.

    Science.gov (United States)

    Park, K Y; Dalakas, M C; Goebel, H H; Ferrans, V J; Semino-Mora, C; Litvak, S; Takeda, K; Goldfarb, L G

    2000-11-01

    Desmin myopathy is a hereditary or sporadic cardiac and skeletal myopathy characterised by intracytoplasmic accumulation of desmin reactive deposits in muscle cells. We have characterised novel splice site mutations in the gene desmin resulting in deletion of the entire exon 3 during the pre-mRNA splicing. Sequencing of cDNA and genomic DNA identified a heterozygous de novo A to G change at the +3 position of the splice donor site of intron 3 (IVS3+3A-->G) in a patient with sporadic skeletal and cardiac myopathy. A G to A transition at the highly conserved -1 nucleotide position of intron 2 affecting the splice acceptor site (IVS2-1G-->A) was found in an unrelated patient with a similar phenotype. Expression of genomic DNA fragments carrying the IVS3+3A-->G and IVS2-1G-->A mutations confirmed that these mutations cause exon 3 deletion. Aberrant splicing leads to an in frame deletion of 32 complete codons and is predicted to result in mutant desmin lacking 32 amino acids from the 1B segment of the alpha helical rod. Functional analysis of the mutant desmin in SW13 (vim-) cells showed aggregation of abnormal coarse clumps of desmin positive material dispersed throughout the cytoplasm. This is the first report on the pathogenic potentials of splice site mutations in the desmin gene.

  2. Width of gene expression profile drives alternative splicing.

    Directory of Open Access Journals (Sweden)

    Daniel Wegmann

    Full Text Available Alternative splicing generates an enormous amount of functional and proteomic diversity in metazoan organisms. This process is probably central to the macromolecular and cellular complexity of higher eukaryotes. While most studies have focused on the molecular mechanism triggering and controlling alternative splicing, as well as on its incidence in different species, its maintenance and evolution within populations has been little investigated. Here, we propose to address these questions by comparing the structural characteristics as well as the functional and transcriptional profiles of genes with monomorphic or polymorphic splicing, referred to as MS and PS genes, respectively. We find that MS and PS genes differ particularly in the number of tissues and cell types where they are expressed.We find a striking deficit of PS genes on the sex chromosomes, particularly on the Y chromosome where it is shown not to be due to the observed lower breadth of expression of genes on that chromosome. The development of a simple model of evolution of cis-regulated alternative splicing leads to predictions in agreement with these observations. It further predicts the conditions for the emergence and the maintenance of cis-regulated alternative splicing, which are both favored by the tissue specific expression of splicing variants. We finally propose that the width of the gene expression profile is an essential factor for the acquisition of new transcript isoforms that could later be maintained by a new form of balancing selection.

  3. Splice site mutations in the ATP7A gene.

    Directory of Open Access Journals (Sweden)

    Tina Skjørringe

    Full Text Available Menkes disease (MD is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12 mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation to in vivo results. Ninety-six percent of the mutations identified in 45 patients with classical MD were predicted to have a significant effect on splicing, which concurs with the absence of any detectable wild-type transcript in all 19 patients investigated in vivo. Sixty-seven percent of the mutations identified in 12 patients with milder phenotypes were predicted to have no significant effect on splicing, which concurs with the presence of wild-type transcript in 7 out of 9 patients investigated in vivo. Both the in silico predictions and the in vivo results support the hypothesis previously suggested by us and others, that the presence of some wild-type transcript is correlated to a milder phenotype.

  4. The functional modulation of epigenetic regulators by alternative splicing

    Directory of Open Access Journals (Sweden)

    Martínez-Balbás Marian

    2007-07-01

    Full Text Available Abstract Background Epigenetic regulators (histone acetyltransferases, methyltransferases, chromatin-remodelling enzymes, etc play a fundamental role in the control of gene expression by modifying the local state of chromatin. However, due to their recent discovery, little is yet known about their own regulation. This paper addresses this point, focusing on alternative splicing regulation, a mechanism already known to play an important role in other protein families, e.g. transcription factors, membrane receptors, etc. Results To this end, we compiled the data available on the presence/absence of alternative splicing for a set of 160 different epigenetic regulators, taking advantage of the relatively large amount of unexplored data on alternative splicing available in public databases. We found that 49 % (70 % in human of these genes express more than one transcript. We then studied their alternative splicing patterns, focusing on those changes affecting the enzyme's domain composition. In general, we found that these sequence changes correspond to different mechanisms, either repressing the enzyme's function (e.g. by creating dominant-negative inhibitors of the functional isoform or creating isoforms with new functions. Conclusion We conclude that alternative splicing of epigenetic regulators can be an important tool for the function modulation of these enzymes. Considering that the latter control the transcriptional state of large sets of genes, we propose that epigenetic regulation of gene expression is itself strongly regulated by alternative splicing.

  5. RNA splicing is responsive to MBNL1 dose.

    Directory of Open Access Journals (Sweden)

    Sonali P Jog

    Full Text Available Myotonic dystrophy (DM1 is a highly variable, multi-system disorder resulting from the expansion of an untranslated CTG tract in DMPK. In DM1 expanded CUG repeat RNAs form hairpin secondary structures that bind and aberrantly sequester the RNA splice regulator, MBNL1. RNA splice defects resulting as a consequence of MBNL1 depletion have been shown to play a key role in the development of DM1 pathology. In patient populations, both the number and severity of DM1 symptoms increase broadly as a function of CTG tract length. However significant variability in the DM1 phenotype is observed in patients encoding similar CTG repeat numbers. Here we demonstrate that a gradual decrease in MBNL1 levels results both in the expansion of the repertoire of splice defects and an increase in the severity of the splice alterations. Thus, MBNL1 loss does not have an all or none outcome but rather shows a graded effect on the number and severity of the ensuing splice defects. Our results suggest that once a critical threshold is reached, relatively small dose variations of free MBNL1 levels, which may reflect modest changes in the size of the CUG tract or the extent of hairpin secondary structure formation, can significantly alter the number and severity of splice abnormalities and thus contribute to the phenotype variability observed in DM1 patients.

  6. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    Directory of Open Access Journals (Sweden)

    Zodwa Dlamini

    2015-11-01

    Full Text Available Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 family have alternatively spliced isoforms that lack important active domains. These isoforms can play a negative regulatory role by binding to and inhibiting the pro-apoptotic forms. Alternative splicing is observed to be increased in various cardiovascular diseases with the level of alternate transcripts increasing elevated in diseased hearts compared to healthy subjects. In many cases these isoforms appear to be the underlying cause of the disease, while in others they may be induced in response to cardiovascular pathologies. Regardless of this, the detection of alternate splicing events in the heart can serve as useful diagnostic or prognostic tools, while those splicing events that seem to play a causative role in cardiovascular disease make attractive future drug targets.

  7. Blast Quantification Using Hopkinson Pressure Bars.

    Science.gov (United States)

    Clarke, Samuel D; Fay, Stephen D; Rigby, Samuel E; Tyas, Andrew; Warren, James A; Reay, Jonathan J; Fuller, Benjamin J; Gant, Matthew T A; Elgy, Ian D

    2016-07-05

    Near-field blast load measurement presents an issue to many sensor types as they must endure very aggressive environments and be able to measure pressures up to many hundreds of megapascals. In this respect the simplicity of the Hopkinson pressure bar has a major advantage in that while the measurement end of the Hopkinson bar can endure and be exposed to harsh conditions, the strain gauge mounted to the bar can be affixed some distance away. This allows protective housings to be utilized which protect the strain gauge but do not interfere with the measurement acquisition. The use of an array of pressure bars allows the pressure-time histories at discrete known points to be measured. This article also describes the interpolation routine used to derive pressure-time histories at un-instrumented locations on the plane of interest. Currently the technique has been used to measure loading from high explosives in free air and buried shallowly in various soils.

  8. How Different are Normal and Barred Spirals?

    CERN Document Server

    Bergh, Sidney van den

    2011-01-01

    No significant color differences are found between normal and barred spirals over the range of Hubble stages a - ab - b - bc. Furthermore, no significant difference is seen between the luminosity distributions of normal and barred galaxies over the same range of Hubble stages. However, SBc galaxies are found to be systematically fainter than Sc galaxies at 99% confidence. The observation that normal and barred spirals with Hubble stages a - ab - b - bc have indistinguishable intrinsic colors hints at the possibility that the bars in such spiral galaxies might be ephemeral structures. Finally, it is pointed out that lenticular galaxies of types S0 and SB0 are systematically fainter than are other early-type galaxies, suggesting that such galaxies are situated on evolutionary tracks that differ systematically from those of galaxies that lie along the E - Sa - Sb -Sc and E - SBa - SBb - SBc sequences.

  9. Measurement of the B0bar Lifetime and the B0B0bar Oscillation Frequency Using Partially Reconstructed B0bar --> D*+ ell- nu-bar Decays

    CERN Document Server

    Aubert, B; Abrams, G S; Adye, T; Ahmed, M; Ahmed, S; Alam, M S; Albert, J; Aleksan, Roy; Allen, M T; Allison, J; Allmendinger, T; Altenburg, D; Andreassen, R; Andreotti, M; Angelini, C; Anulli, F; Arnaud, N; Aston, D; Azzolini, V; Baak, M; Back, J J; Bailey, S; Baldini-Ferroli, R; Band, H R; Banerjee, Sw; Barate, R; Bard, D J; Barlow, N R; Barlow, R J; Barrett, M; Bartoldus, R; Batignani, G; Battaglia, M; Bauer, J M; Beck, T W; Behera, P K; Bellini, F; Benayoun, M; Benelli, G; Berger, N; Bernard, D; Berryhill, J W; Best, D; Bettarini, S; Bettoni, D; Bevan, A J; Bhimji, W; Bhuyan, B; Bianchi, F; Biasini, M; Biesiada, J; Blanc, F; Blaylock, G; Blinov, A E; Blinov, V E; Bloom, P; Bomben, M; Bóna, M; Bondioli, M; Bonneaud, G R; Bosisio, L; Boutigny, D; Bowerman, D A; Boyarski, A M; Boyd, J T; Bozzi, C; Brandenburg, G; Brandt, T; Brau, J E; Breon, A B; Briand, H; Brose, J; Brown, C L; Brown, C M; Brown, D N; Bruinsma, M; Brunet, S; Bucci, F; Buchanan, C; Buchmüller, O L; Bugg, W; Bukin, A D; Bulten, H; Burchat, P R; Burke, J P; Button-Shafer, J; Buzzo, A; Côté, D; Cahn, R N; Calabrese, R; Calcaterra, A; Calderini, G; Campagnari, C; Capra, R; Carpinelli, M; Cartaro, C; Cavoto, G; Cenci, R; Chaisanguanthum, K S; Chao, M; Charles, E; Charles, M J; Chauveau, J; Chavez, C A; Chen, A; Chen, C; Chen, E; Chen, J C; Chen, S; Chen, X; Cheng, B; Cheng, C H; Chevalier, N; Cibinetto, G; Clark, P J; Claus, R; Cochran, J; Coleman, J P; Contri, R; Convery, M R; Cormack, C M; Cossutti, F; Cottingham, W N; Couderc, F; Covarelli, R; Cowan, G; Cowan, R; Crawley, H B; Cremaldi, L; Cristinziani, M; Çuhadar-Dönszelmann, T; Cunha, A; D'Orazio, A; Dahmes, B; Dallapiccola, C; Danielson, N; Dasu, S; Datta, M; Dauncey, P D; David, P; Davier, M; Davis, D BrownC L; Day, C T; De Groot, N; De Nardo, N; Cavallo, G; Del Buono, L; Della Ricca, G; Di Lodovico, F; Di Marco, E; Dickopp, M; Dingfelder, J C; Dittongo, S; Dong, D; Dorfan, J; Dorigo, A; Druzhinin, V P; Dubitzky, R S; Dubois-Felsmann, G P; Dujmic, D; Dunwoodie, W M; Dvoretskii, A; Eckhart, E A; Eckmann, R; Edwards, A J; Egede, U; Eichenbaum, A M; Eigen, G; Eisner, A M; Elmer, P; Emery, S; Ernst, J A; Eschenburg, V; Eschrich, I; Eyges, V; Faccini, R; Fan, S; Farbin, A; Feltresi, E; Ferrarotto, F; Ferroni, F; Field, R C; Finocchiaro, G; Flacco, C J; Flack, R L; Flächer, H U; Flood, K T; Ford, K E; Ford, W T; Forster, I J; Forti, F; Fortin, D; Foulkes, S D; Franek, B; Frey, R; Fritsch, M; Fry, J R; Fulsom, B G; Gabathuler, E; Gaidot, A; Gaillard, J R; Galeazzi, F; Gallo, F; Gamba, D; Gamet, R; Gan, K K; Ganzhur, S F; Gary, J W; Gaspero, M; Gatto, F; Fabozzi, C; George, K A; Gill, M S; Giorgi, M A; Giraud, P F; Giroux, X; Gladney, L; Glanzman, T; Godang, R; Goetzen, K; Golubev, V B; Gopal, G P; Gowdy, S J; Gradl, W; Graham, M; Grancagnolo, S; Graugès-Pous, E; Graziani, G; Green, M G; Grenier, P; Gritsan, A V; Grosdidier, G; Groysman, Y; Guo, Q H; Hadavand, H K; Hadig, T; Haire, M; Halyo, V; Hamano, K; Hamel de Monchenault, G; Hamon, O; Harrison, P F; Harrison, T J; Hart, A J; Hartfiel, B L; Hast, C; Hauke, A; Hawkes, C M; Hearty, C; Held, T; Hertzbach, S S; Heusch, C A; Hill, E J; Hitlin, D G; Höcker, A; Hodgkinson, M C; Hollar, J J; Hong, T M; Honscheid, K; Hopkins, D A; Hrynóva, T; Hufnagel, D; Hulsbergen, W D; Hutchcroft, D E; Igonkina, O; Innes, W R; Izen, J M; Jackson, P D; Jackson, P S; Jacobsen, R G; Jawahery, A; Jayatilleke, S M; John, M J J; Johnson, J R; Judd, D; Kadel, R W; Kadyk, J; Kagan, H; Karyotakis, Yu; Kass, R; Kelly, M P; Kelsey, M H; Kerth, L T; Khan, A; Kim, H; Kim, P; Kirkby, D; Kitayama, I; Klose, V; Knecht, N S; Koch, H; Kocian, M L; Koeneke, K; Kofler, R; Kolomensky, Yu G; Koptchev, V B; Kovalskyi, D; Kowalewski, R V; Kozanecki, Witold; Kravchenko, E A; Krishnamurthy, M; Kroeger, R; Kroseberg, J; Kukartsev, G; Kutter, P E; Kyberd, P; Lacker, H M; Lae, C K; Lafferty, G D; Lanceri, L; Lange, D J; Langenegger, U; Lankford, A J; Latham, T E; Lau, Y P; Lazzaro, A; Le Diberder, F R; Lees, J P; Legendre, M; Leith, D W G S; Lepeltier, V; Leruste, P; Levesque, J A; Lewandowski, B; Li Gioi, L; Li, H; Li, X; Libby, J; Lillard, V; Lista, L; Liu, R; Lo Secco, C P; Jessop, J M; Lo Vetere, M; Lockman, W S; Lombardo, V; London, G W; Long, O; Lou, X C; Lü, C; Lu, M; Luitz, S; Luppi, E; Lusiani, A; Lüth, V; Lutz, A M; Lynch, G; Lynch, H L; MacFarlane, D B; Macri, M; Mader, W F; Majewski, S A; Malcles, J; Mallik, U; Mancinelli, G; Mandelkern, M A; Marchiori, G; Margoni, M; Marks, J; Marsiske, H; Martínez-Vidal, F; Mattison, T S; Mayer, B; Mazur, M A; Mazzoni, M A; McKenna, J A; McMahon, T R; Meadows, B T; Mellado, B; Messner, R; Meyer, W T; Mihályi, A; Mir, L M; Mohanty, G B; Mohapatra, A K; Mommsen, R K; Monge, M R; Monorchio, D; Moore, T B; Morandin, M; Morgan, S E; Morganti, M; Morganti, S; Morii, M; Morton, G W; Muheim, F; Müller, D R; Naisbit, M T; Narsky, I; Nash, J A; Nauenberg, U; Nd, 14 40; Neal, H; Negrini, M; Neri, N; Nesom, G; Nicholson, H; Nikolich, M B; Nogowski, R; O'Grady, C P; Ocariz, J; Oddone, P J; Ofte, I; Olaiya, E O; Olivas, A; Olsen, J; Onuchin, A P; Orimoto, T J; Otto, S; Oyanguren, A; Ozcan, V E; Paar, H P; Pacetti, S; Palano, A; Palombo, F; Pan, Y; Panetta, J; Panvini, R S; Paoloni, E; Paolucci, P; Pappagallo, M; Parry, R J; Passaggio, S; Patel, P M; Patrignani, C; Patteri, P; Payne, D J; Pelizaeus, M; Perazzo, A; Perl, M; Peters, K; Petersen, B A; Petersen, T C; Petzold, A; Piatenko, T; Piccolo, D; Piccolo, I M; Peruzzi, M; Piemontese, L; Pierini, M; Pioppi, M; Piredda, G; Plaszczynski, S; Playfer, S; Poireau, V; Polci, F; Pompili, A; Porter, F C; Posocco, M; Potter, C T; Prell, S; Prepost, R; Pripstein, M; Pulliam, T; Purohit, M V; Qi, N D; Rahatlou, S; Rahimi, A M; Rama, M; Rankin, P; Ratcliff, B N; Raven, G; Reidy, J; Ricciardi, S; Richman, J D; Ritchie, J L; Rizzo, G; Roat, C; Roberts, D A; Robertson, S H; Robutti, E; Rodier, S; Roe, N A; Röthel, W; Ronan, M T; Roney, J M; Rong, G; Roodman, A; Roos, L; Rosenberg, E I; Rotondo, M; Roudeau, P; Rubin, A E; Ruddick, W O; Ryd, A; Sacco, R; Saeed, M A; Safai-Tehrani, F; Saleem, M; Salnikov, A A; Salvatore, F; Samuel, A; Sanders, D A; Santroni, A; Saremi, S; Satpathy, A; Schalk, T; Schenk, S; Schindler, R H; Schofield, K C; Schott, G; Schrenk, S; Schröder, H; Schröder, T; Schubert, J; Schubert, K R; Schumm, B A; Schune, M H; Schwiening, J; Schwierz, R; Schwitters, R F; Sciacca, C; Sciolla, G; Seiden, A; Sekula, S J; Serednyakov, S I; Sharma, V; Shen, B C; Simani, M C; Simi, G; Simonetto, F; Sinev, N B; Skovpen, Yu I; Smith, A J S; Smith, J G; Snoek, H L; Snyder, A; Sobie, R J; Soffer, A; Sokoloff, M D; Solodov, E P; Spaan, B; Spanier, S M; Spitznagel, M; Spradlin, P; Stängle, H; Steinke, M; Stelzer, J; Stocchi, A; Stoker, D P; Stroili, R; Strom, D; Strube, J; Stugu, B; Su, D; Sullivan, M K; Summers, D J; Sundermann, J E; Suzuki, K; Swain, S; Tan, P; Taras, P; Taylor, F; Taylor, G P; Telnov, A V; Teodorescu, L; Ter-Antonian, R; Therin, G; Thiebaux, C; Thompson, J M; Tisserand, V; Toki, W H; Torrence, E; Tosi, S; Touramanis, C; Ulmer, K A; Uwer, U; Vasileiadis, G; Vasseur, G; Vavra, J; Vazquez, W P; Verderi, M; Verkerke, W; Viaud, B; Vitale, L; Voci, C; Voena, C; Wagner, G; Wagner, S R; Wagoner, D E; Waldi, R; Walsh, J; Wang, K; Wang, P; Wappler, F R; Watson, A T; Weaver, M; Weidemann, A W; Weinstein, A J R; Wenzel, W A; Wilden, L; Williams, D C; Williams, J C; Willocq, S; Wilson, F F; Wilson, J R; Wilson, M G; Wilson, R J; Wisniewski, W J; Wittgen, M; Won, E; Wong, Q K; Wormser, G; Wright, D H; Wright, D M; Wu, J; Wu, S L; Xie, Y; Yamamoto, R K; Yarritu, A K; Ye, S; Yéche, C; Yi, J; Yi, K; Young, C C; Yu, Z; Yumiceva, F X; Yushkov, A N; Zain, S B; Zallo, A; Zeng, Q; Zghiche, A; Zhang, J; Zhang, L; Zhao, H W; Zhu, Y S; Zito, M; De Sangro, R; Del Re, D; La Vaissière, C de; Von Wimmersperg-Töller, J H

    2006-01-01

    We present a simultaneous measurement of the B0bar lifetime tau_B0 and B0B0bar oscillation frequency Delta_md. We use a sample of about 50000 partially reconstructed B0bar --> D*+ ell- nu-bar decays identified with the BABAR detector at the PEP-II e+e- storage ring at SLAC. The flavor of the other B meson in the event is determined from the charge of another high-momentum lepton. The results are tau_B0 = (1.504 +/- 0.013 (stat) {+0.018}/{-0.013} (syst)) ps, Delta_md = (0.511 +/- 0.007 (stat) {+0.007}/{-0.006} (syst)) ps-1

  10. Bus Stops, Ctran amd Rtp Bus Stops, Published in 2007, 1:1200 (1in=100ft) scale, Town of Cary NC.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Stops dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as...

  11. Bus Stops, Maryland Transit Administration Local and Commuter Bus Stops, Published in 2013, 1:24000 (1in=2000ft) scale, Maryland Transit Administration.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Stops dataset, published at 1:24000 (1in=2000ft) scale, was produced all or in part from Other information as of 2013. It is described as 'Maryland Transit...

  12. Bus Routes, Maryland Transit Administration Local and Commuter Bus Routes, Published in 2013, 1:24000 (1in=2000ft) scale, Maryland Transit Administration.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, published at 1:24000 (1in=2000ft) scale, was produced all or in part from Other information as of 2013. It is described as 'Maryland Transit...

  13. Bus Routes, This shapefile contains lines that follow the path of the existing bus routes, Published in 2006, Charlotte Area Transit System.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Bus Routes dataset, was produced all or in part from Other information as of 2006. It is described as 'This shapefile contains lines that follow the path of...

  14. Chocolate Bars Based on Human Nutritional Requirements

    OpenAIRE

    Robson, Anthony,

    2013-01-01

    International audience; Key Points * The nutritional value of chocolate bars should be based on the nutritional value of the low energy dense late Paleolithic human diet to help reduce mental ill health, obesity, and other postprandial insults. * Current chocolate bars have a high energy density (>2 kcal/g). * Cocoa can be sweetened by the addition of calorie-free Purefruit™ (Tate & Lyle) monk fruit ( Siraitia grosvenorii ) extract. PUREFRUIT™ is approximately 200 times sweeter than sugar and...

  15. Intelligent bar chart plagiarism detection in documents.

    Science.gov (United States)

    Al-Dabbagh, Mohammed Mumtaz; Salim, Naomie; Rehman, Amjad; Alkawaz, Mohammed Hazim; Saba, Tanzila; Al-Rodhaan, Mznah; Al-Dhelaan, Abdullah

    2014-01-01

    This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR). By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts.

  16. Bar pattern speeds in CALIFA galaxies. I. Fast bars across the Hubble sequence

    Science.gov (United States)

    Aguerri, J. A. L.; Méndez-Abreu, J.; Falcón-Barroso, J.; Amorin, A.; Barrera-Ballesteros, J.; Cid Fernandes, R.; García-Benito, R.; García-Lorenzo, B.; González Delgado, R. M.; Husemann, B.; Kalinova, V.; Lyubenova, M.; Marino, R. A.; Márquez, I.; Mast, D.; Pérez, E.; Sánchez, S. F.; van de Ven, G.; Walcher, C. J.; Backsmann, N.; Cortijo-Ferrero, C.; Bland-Hawthorn, J.; del Olmo, A.; Iglesias-Páramo, J.; Pérez, I.; Sánchez-Blázquez, P.; Wisotzki, L.; Ziegler, B.

    2015-04-01

    Context. The bar pattern speed (Ωb) is defined as the rotational frequency of the bar, and it determines the bar dynamics. Several methods have been proposed for measuring Ωb. The non-parametric method proposed by Tremaine & Weinberg (1984, ApJ, 282, L5; TW) and based on stellar kinematics is the most accurate. This method has been applied so far to 17 galaxies, most of them SB0 and SBa types. Aims: We have applied the TW method to a new sample of 15 strong and bright barred galaxies, spanning a wide range of morphological types from SB0 to SBbc. Combining our analysis with previous studies, we investigate 32 barred galaxies with their pattern speed measured by the TW method. The resulting total sample of barred galaxies allows us to study the dependence of Ωb on galaxy properties, such as the Hubble type. Methods: We measured Ωb using the TW method on the stellar velocity maps provided by the integral-field spectroscopy data from the CALIFA survey. Integral-field data solve the problems that long-slit data present when applying the TW method, resulting in the determination of more accurate Ωb. In addition, we have also derived the ratio ℛ of the corotation radius to the bar length of the galaxies. According to this parameter, bars can be classified as fast (ℛ 1.4). Results: For all the galaxies, ℛ is compatible within the errors with fast bars. We cannot rule out (at 95% level) the fast bar solution for any galaxy. We have not observed any significant trend between ℛ and the galaxy morphological type. Conclusions: Our results indicate that independent of the Hubble type, bars have been formed and then evolve as fast rotators. This observational result will constrain the scenarios of formation and evolution of bars proposed by numerical simulations.

  17. UNIVERSALIZATION OF BUS BODY FACING DETAILS AND PRODUCTION TOOLING FOR THEIR MANUFACTURING

    Directory of Open Access Journals (Sweden)

    M. Taranenko

    2009-01-01

    Full Text Available The directions concerning bus body production costs reduction based on large-size facing details stamping on electric-hydraulic presses are considered. The engineering of unified large-size facing detail blanks, manufacturing processes and variants of unified technological tooling for bus body facing details production, including simple- or multi-type details are described.

  18. Spaceflight qualification of the SEDS MIL-STD-1773 fiber optic data bus

    Science.gov (United States)

    Flanegan, Mark C.

    1993-09-01

    The small explorer (SMEX) project at Goddard Space Flight Center (GSFC) launched the first spaceflight implementation of the MIL-STD-1773 fiber optic data bus on the Solar Anomalous Magnetospheric Particle Explorer (SAMPEX) in July of 1992. The Small Explorer Data System (SEDS), of which the MIL-STD-1773 data bus is a part, has been successful. The MIL-STD-1773 bus is the implementation of the MIL-STD-1553 protocol using fiber optics. Advantages of the fiber optic bus over the electrical bus include lower power, lower weight, and immunity from EMI/RFI. It does not radiate electrical or magnetic fields. It is a nonconductor so it cannot conduct electrical noise into or from a subsystem. This is particularly advantageous on a spacecraft with very sensitive instruments which are often susceptible to electrical interference. Although the MIL-STD-1773 bus is a 1 Mbps bus like the MIL-STD-1553 bus, the fiber optics also provide a path to the much higher rate systems required in upcoming NASA missions.

  19. Converter Power Density Increase using Low Inductive Integrated DC-link Capacitor/Bus

    DEFF Research Database (Denmark)

    Trintis, Ionut; Franke, Toke; Rannested, Bjørn;

    2015-01-01

    The power losses in switching devices have a direct effect on the maximum converter power. For a voltage source converter, the DC-link bus has a major influence on the power loss and safe operating area of the power devices. The Power Ring Film CapacitorTM integrated with an optimized bus structu...

  20. 49 CFR 571.221 - Standard No. 221, School bus body joint strength.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Standard No. 221, School bus body joint strength. 571.221 Section 571.221 Transportation Other Regulations Relating to Transportation (Continued... STANDARDS Federal Motor Vehicle Safety Standards § 571.221 Standard No. 221, School bus body joint...

  1. 49 CFR 571.221 - Standard No. 221; School bus body joint strength.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Standard No. 221; School bus body joint strength... STANDARDS Federal Motor Vehicle Safety Standards § 571.221 Standard No. 221; School bus body joint strength. S1. Scope. This standard establishes requirements for the strength of the body panel joints in...

  2. Evoking Emotions and Unpacking Layered Histories through Young Children's Illustrations of Racial Bus Segregation

    Science.gov (United States)

    Kuby, Candace R.

    2013-01-01

    Drawing on theories of multi-modality and critical visual literacy, this article focuses on images that five-and six year-olds painted in a class-made book, Voice on the Bus, about racial segregation. The article discusses how children used illustrations to convey their understandings of Rosa Parks' bus arrest in Alabama. A post-structural view…

  3. This Could Save Your Child's Life. A School Bus Handrail Handbook.

    Science.gov (United States)

    National Highway Traffic Safety Administration (DOT), Washington, DC.

    School-bus handrails have had the same basic design for more than 30 years. However, students' recent propensity to wear oversized and baggy clothing increases the likelihood that clothing or accessories may become snagged on bus handrails. Children across the United States have been injured or killed when their clothing or accessories caught in…

  4. Bus Operation Monitoring Oriented Public Transit Travel Index System and Calculation Models

    Directory of Open Access Journals (Sweden)

    Jiancheng Weng

    2013-01-01

    Full Text Available This study proposed a two-dimensional index system which is concerned essentially with urban travel based on travel modes and user satisfaction. First, the public transit was taken as an example to describe the index system establishing process. In consideration of convenience, rapid, reliability, comfort, and safety, a bus service evaluation index system was established. The indicators include the N-minute coverage of bus stops, average travel speed, and fluctuation of travel time between stops and bus load factor which could intuitively describe the characteristics of public transport selected to calculate bus travel indexes. Then, combined with the basic indicators, the calculation models of Convenience Index (CI, Rapid Index (RI, Reliability Index (RBI, and Comfort Index (CTI were established based on the multisource data of public transit including the real-time bus GPS data and passenger IC card data. Finally, a case study of Beijing bus operation evaluation and analysis was conducted by taking real bus operation data including GPS data and passenger transaction recorder (IC card data. The results showed that the operation condition of the public transit was well reflected and scientifically classified by the bus travel index models.

  5. Bus Implementation Using New Low Power PFSCL Tristate Buffers

    Directory of Open Access Journals (Sweden)

    Neeta Pandey

    2016-01-01

    Full Text Available This paper proposes new positive feedback source coupled logic (PFSCL tristate buffers suited to bus applications. The proposed buffers use switch to attain high impedance state and modify the load or the current source section. An interesting consequence of this is overall reduction in the power consumption. The proposed tristate buffers consume half the power compared to the available switch based counterpart. The issues with available PFSCL tristate buffers based bus implementation are identified and benefits of employing the proposed tristate buffer topologies are put forward. SPICE simulation results using TSMC 180 nm CMOS technology parameters are included to support the theoretical formulations. The performance of proposed tristate buffer topologies is examined on the basis of propagation delay, output enable time, and power consumption. It is found that one of the proposed tristate buffer topology outperforms the others in terms of all the performance parameters. An examination of behavior of available and the proposed PFSCL tristate buffer topologies under parameter variations and mismatch shows a maximum variation of 14%.

  6. Reducing transit bus emissions: Alternative fuels or traffic operations?

    Science.gov (United States)

    Alam, Ahsan; Hatzopoulou, Marianne

    2014-06-01

    In this study, we simulated the operations and greenhouse gas (GHG) emissions of transit buses along a busy corridor and quantified the effects of two different fuels (conventional diesel and compressed natural gas) as well as a set of driving conditions on emissions. Results indicate that compressed natural gas (CNG) reduces GHG emissions by 8-12% compared to conventional diesel, this reduction could increase to 16% with high levels of traffic congestion. However, the benefits of switching from conventional diesel to CNG are less apparent when the road network is uncongested. We also investigated the effects of bus operations on emissions by applying several strategies such as transit signal priority (TSP), queue jumper lanes, and relocation of bus stops. Results show that in congested conditions, TSP alone can reduce GHG emissions by 14% and when combined with improved technology; a reduction of 23% is achieved. The reduction benefits are even more apparent when other transit operational improvements are combined with TSP. Finally a sensitivity analysis was performed to investigate the effect of operational improvements on emissions under varying levels of network congestion. We observe that under “extreme congestion”, the benefits of TSP decrease.

  7. A programmable systolic trigger processor for FERA-bus data

    Science.gov (United States)

    Appelquist, G.; Hovander, B.; Sellden, B.; Bohm, C.

    1992-09-01

    A generic CAMAC based trigger processor module for fast processing of large amounts of Analog to Digital Converter (ADC) data was designed. This module was realized using complex programmable gate arrays. The gate arrays were connected to memories and multipliers in such a way that different gate array configurations can cover a wide range of module applications. Using this module, it is possible to construct complex trigger processors. The module uses both the fast ECL FERA bus and the CAMAC bus for inputs and outputs. The latter is used for set up and control but may also be used for data output. Large numbers of ADC's can be served by a hierarchical arrangement of trigger processor modules which process ADC data with pipeline arithmetics and produce the final result at the apex of the pyramid. The trigger decision is transmitted to the data acquisition system via a logic signal while numeric results may be extracted by the CAMAC controller. The trigger processor was developed for the proposed neutral particle search. It was designed to serve as a second level trigger processor. It was required to correct all ADC raw data for efficiency and pedestal, calculate the total calorimeter energy, obtain the optimal time of flight data, and calculate the particle mass. A suitable mass cut would then deliver the trigger decision.

  8. Studying customer loyalty at Daewoo express bus service, Pakistan

    Directory of Open Access Journals (Sweden)

    Usman Yousaf

    2013-09-01

    Full Text Available Background: Meeting service quality standards and striving for loyalty are two critical areas which have until now been overlooked by both the passenger transport industry and academia in Pakistan therefore the study sheds useful light on an issue hitherto untouched. Therefore increased sample size will help in increasing generalizability of study. Methods: This paper is designed to study the level of loyalty at Daewoo Express Bus Service in Sargodha by measuring customer satisfaction. Researchers used a small sample of only 96 respondents (passengers and only studies customer's behavior in the service quality of Daewoo Express Bus Service however it may differ in other passenger transport services. Results and conclusions: This paper reveals that there is a positive and significant relationship between loyalty (dependant variable and price perceptions, reliability, retrieving and smoothing (independent variables. However, the study found that reliability of services is the most important dimension among other independent variables (price perceptions, smoothing, reliability, retrieving effecting customers loyalty at Daewoo Express. Paper under consideration would surely assist Daewoo`s management team to take care of the loopholes existing in the current service level and likely threats which Daewoo might face.

  9. Improved blood pressure control among school bus drivers with hypertension.

    Science.gov (United States)

    Doyle, Joseph; Severance-Fonte, Tina; Morandi-Matricaria, Elizabeth; Wogen, Jenifer; Frech-Tamas, Feride

    2010-04-01

    The impact of a hypertension awareness and educational program, BP DownShift, was evaluated among school bus drivers in a southern US state. At baseline (August 2007), blood pressure (BP) measurements, self-reported demographics, and hypertension awareness and management practices were collected from drivers who consented to participate in the study. Interventions included 4 educational mailings, installation of BP machines at all bus terminals, and access to free dietitian consultations and gym memberships. BP was evaluated using Department of Transportation guidelines. BP was remeasured and a survey was administered at follow-up (May 2008). At baseline, 208 drivers consented to the BP screening; 120 (58%) returned for a follow-up assessment. Most participants completing the study were female (73%) and African American (72%). Mean age was 50 years and mean body mass index was 32 kg/m(2); 52% of participants were obese. In all, 58% of participants reported a prior diagnosis of hypertension by a physician, and 63% reported taking antihypertensive medication. Both systolic and diastolic BP (SBP and DBP) were lower at follow-up (135/82 mmHg vs. 145/87 mmHg at baseline; P 10 mmHg, and 44% had a reduction in DBP > 5 mmHg. At follow-up, 58% were controlled to BP improvement in BP control, which may positively impact commercial driver's license recertification as well as improve employee health.

  10. CERPEN FARUK “BUS KOTA” DALAM SEMIOTIK ROLAND BARTHES

    Directory of Open Access Journals (Sweden)

    Ken Widyatwati

    2016-04-01

    Full Text Available Literature called beautiful if it contains the mandate, message, ideas and meanings that are useful for the readers. Short story “Bus Kota” (City Bus containing the mandate, message, ideas and meanings that are useful to the reader. The mandate will be revealed by Roland Barthes' semiotic theory in the book S/Z. Literature has its own allure and text have accrued but not due to independence because of the difference in quality. Texts merge into a unity, or restore any text not as an individual but as a form of plurality. Literature as part of a social system is an expression of the author's sense of the outpouring of public life. Literary works can express themselves in the author's view the social and cultural changes in society because of the development of information technology and rapid. Technological developments and socio-cultural information can change in society so as to affect the psychology of the people. Society must have a thickness of faith not only for themselves, but to be able to maintain equanimity in the life of a pluralistic society

  11. The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration

    Science.gov (United States)

    Choudhury, Rajarshi; Roy, Sreerupa Ghose; Tsai, Yihsuan S.; Tripathy, Ashutosh; Graves, Lee M.; Wang, Zefeng

    2014-01-01

    Alternative splicing of pre-messenger RNA (mRNA) is a critical stage of gene regulation in response to environmental stimuli. Here we show that DAZAP1, an RNA-binding protein involved in mammalian development and spermatogenesis, promotes inclusion of weak exons through specific recognition of diverse cis-elements. The carboxy-terminal proline-rich domain of DAZAP1 interacts with and neutralizes general splicing inhibitors, and is sufficient to activate splicing when recruited to pre-mRNA. This domain is phosphorylated by the MEK/Erk (extracellular signal-regulated protein kinase) pathway and this modification is essential for the splicing regulatory activity and the nuclear/cytoplasmic translocation of DAZAP1. Using mRNA-seq, we identify endogenous splicing events regulated by DAZAP1, many of which are involved in maintaining cell growth. Knockdown or over-expression of DAZAP1 causes a cell proliferation defect. Taken together, these studies reveal a molecular mechanism that integrates splicing control into MEK/Erk-regulated cell proliferation.

  12. Biochemical identification of new proteins involved in splicing repression at the Drosophila P-element exonic splicing silencer

    Science.gov (United States)

    Horan, Lucas; Yasuhara, Jiro C.; Kohlstaedt, Lori A.; Rio, Donald C.

    2015-01-01

    Splicing of the Drosophila P-element third intron (IVS3) is repressed in somatic tissues due to the function of an exonic splicing silencer (ESS) complex present on the 5′ exon RNA. To comprehensively characterize the mechanisms of this alternative splicing regulation, we used biochemical fractionation and affinity purification to isolate the silencer complex assembled in vitro and identify the constituent proteins by mass spectrometry. Functional assays using splicing reporter minigenes identified the proteins hrp36 and hrp38 and the cytoplasmic poly(A)-binding protein PABPC1 as novel functional components of the splicing silencer. hrp48, PSI, and PABPC1 have high-affinity RNA-binding sites on the P-element IVS3 5′ exon, whereas hrp36 and hrp38 proteins bind with low affinity to the P-element silencer RNA. RNA pull-down and immobilized protein assays showed that hrp48 protein binding to the silencer RNA can recruit hrp36 and hrp38. These studies identified additional components that function at the P-element ESS and indicated that proteins with low-affinity RNA-binding sites can be recruited in a functional manner through interactions with a protein bound to RNA at a high-affinity binding site. These studies have implications for the role of heterogeneous nuclear ribonucleoproteins (hnRNPs) in the control of alternative splicing at cis-acting regulatory sites. PMID:26545814

  13. Study on the optimum design of bus window pillar join40t; Bus window pillar ketsugo buzai no saiteki sekkei ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Tomioka, N. [Nihon University, Tokyo (Japan); Lim, S.; Kim, M.; Lee, H.; Kang, S.; Bae, D.

    1997-10-01

    Automobile body structure is generally assembled using various spot welded box sectional members. Especially, in the case of the bus, the shape of window pillar joint is assembled m T-type. This T-type member has some problem such as high stress concentration, low fatigue strength and structural rigidity. Therefore, in this report, performed a study on the optimum design of the bus window pillar joint for such problem by FEM analysis and experiments. 1 ref., 10 figs., 2 tabs.

  14. Bus rapid transit (BRT) and transitoriented development (TOD) : How to transform and adjust the Swedish cities for attractive bus systems like BRT? What demands BRT?

    OpenAIRE

    Stojanovski, Todor

    2013-01-01

    Bus rapid transit (BRT) is an innovative bus system with sophisticated vehicles and inflexible busways integrated in the cities, high capacity and high quality, high speed and frequency, distinctive image and comfort. Many in Sweden believe that is impossible to introduce BRT, even though the Swedish towns and cities can benefit from the image, speed and frequency that BRT symbolizes. The archipelago-like urbanization, urban sprawl and the uncompetitive journey times of public transportation ...

  15. Hot Disks And Delayed Bar Formation

    CERN Document Server

    Sheth, Kartik; Elmegreen, Debra Meloy; Elmegreen, Bruce G; Athanassoula, E; Weiner, Ben

    2012-01-01

    We present observational evidence for the inhibition of bar formation in dispersion-dominated (dynamically hot) galaxies by studying the relationship between galactic structure and host galaxy kinematics in a sample of 257 galaxies between 0.1 $<$ z $\\leq$ 0.84 from the All-Wavelength Extended Groth Strip International Survey (AEGIS) and the Deep Extragalactic Evolutionary Probe 2 (DEEP2) survey. We find that bars are preferentially found in galaxies that are massive and dynamically cold (rotation-dominated) and on the stellar Tully-Fisher relationship, as is the case for barred spirals in the local Universe. The data provide at least one explanation for the steep ($\\times$3) decline in the overall bar fraction from z=0 to z=0.84 in L$^*$ and brighter disks seen in previous studies. The decline in the bar fraction at high redshift is almost exclusively in the lower mass (10 $<$ log M$_{*}$(\\Msun)$<$ 11), later-type and bluer galaxies. A proposed explanation for this "downsizing" of the bar formation ...

  16. Learning the sequence determinants of alternative splicing from millions of random sequences.

    Science.gov (United States)

    Rosenberg, Alexander B; Patwardhan, Rupali P; Shendure, Jay; Seelig, Georg

    2015-10-22

    Most human transcripts are alternatively spliced, and many disease-causing mutations affect RNA splicing. Toward better modeling the sequence determinants of alternative splicing, we measured the splicing patterns of over two million (M) synthetic mini-genes, which include degenerate subsequences totaling over 100 M bases of variation. The massive size of these training data allowed us to improve upon current models of splicing, as well as to gain new mechanistic insights. Our results show that the vast majority of hexamer sequence motifs measurably influence splice site selection when positioned within alternative exons, with multiple motifs acting additively rather than cooperatively. Intriguingly, motifs that enhance (suppress) exon inclusion in alternative 5' splicing also enhance (suppress) exon inclusion in alternative 3' or cassette exon splicing, suggesting a universal mechanism for alternative exon recognition. Finally, our empirically trained models are highly predictive of the effects of naturally occurring variants on alternative splicing in vivo.

  17. Possible Signatures of New Physics in e+e- and bar bar{p}p Collisions

    Science.gov (United States)

    Senju, H.

    1998-12-01

    A preon model with preonic charge predicts many unique new particles. Among them, several are expected to be relatively light, including the fermion lS, which is a stable WIMP, bosons U0 and U+ and the lepto-quark fermion q'. The production of these particles in e+e- and bar{p}p collisions is discussed, focusing on e+e- --> UlS(e) and bar{p}p --> bar{q}'q' + X. A signature of the latter is dilepton + 2 charm jets + missing energy. A discussion on reported unusual events in the dilepton + jets sample is made based on bar{q}'q' production.

  18. Dynamical Calculations of bar K and MULTI-bar K Nuclei

    Science.gov (United States)

    Gazda, D.; Mareš, J.; Friedman, E.; Gal, A.

    We report on our recent calculations of bar K and multi-bar K nuclei. Calculations were performed fully self-consistently across the periodic table using the relativistic mean-field approach. We aimed at detailed analysis of dynamical processes and various thresholds that determine the K- absorption width. Further, we studied the behavior of the nuclear medium under the influence of increasing strangeness in order to search for bar K condensation precursor phenomena. Last, we explored possibly self-bound strange hadronic configurations consisting of neutrons and bar K0 mesons and studied their properties.

  19. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Second Results Report and Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-10-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This is the second results report for the AT fuel cell bus since it was placed in service, and it focuses on the newest data analysis and lessons learned since the previous report. The appendices, referenced in the main report, provide the full background for the evaluation. They will be updated as new information is collected but will contain the original background material from the first report.

  20. A Study of BUS Architecture Design for Controller of Nuclear Power Plant Using FPGA

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dongil; Yun, Donghwa; Hwang, Sungjae; Kim, Myeongyun; Lee, Dongyun [PONUTech Co. Ltd., Seoul (Korea, Republic of)

    2014-05-15

    CPU (Central Processing Unit) operating speed and communication rate have been more technically improved than before. However, whole system is been a degradation of performance by electronic and structural limitation of parallel bus. Transmission quantity and speed have a limit and need arbiter in order to do arbitration because several boards shared parallel bus. Arbiter is a high complexity in implementing so it increases component per chip. If a parallel bus uses, it will occurs some problems what are reflection noise, power/ground noise (or ground bounce) as SSN (Simultaneous Switching Noise) and crosstalk noise like magnetic coupling. In this paper, in order to solve a problem of parallel bus in controller of NPP (Nuclear Power Plant), proposes the bus architecture design using FPGA (Field Programmable Gate Array) based on LVDS (Low Voltage Differential Signaling)

  1. Occupational safety conditions of bus drivers in Metro Manila, the Philippines.

    Science.gov (United States)

    Santos, Jewelle Ann; Lu, Jinky Leilanie

    2016-12-01

    The study looks into the occupational safety and working conditions among bus drivers in Metro Manila, the Philippines. Quantitative data were collected through survey interviews of 95 bus drivers using the stratified sampling technique. Results showed that bus drivers worked an average of 16 h/day and were engaged in risky driving behaviors such as over-speeding and road racing in order to reach their quota for the day. Fifty-nine percent experienced work-related accidents, with a mean of three accidents. The most common accident was hitting another vehicle followed by side swipe. The accidents were blamed on other drivers, followed by vehicle defect, inattentiveness and tiredness/micro-sleep or sudden involuntary sleep while driving. The most common health symptoms experienced by the bus drivers were fatigue, back pain, and cough and colds. This study underlines the need for an occupational health and safety program for bus drivers in the Philippines.

  2. Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides.

    Science.gov (United States)

    Igreja, Susana; Clarke, Luka A; Botelho, Hugo M; Marques, Luís; Amaral, Margarida D

    2016-02-01

    Cystic fibrosis (CF), the most common life-threatening genetic disease in Caucasians, is caused by ∼2,000 different mutations in the CF transmembrane conductance regulator (CFTR) gene. A significant fraction of these (∼13%) affect pre-mRNA splicing for which novel therapies have been somewhat neglected. We have previously described the effect of the CFTR splicing mutation c.2657+5G>A in IVS16, showing that it originates transcripts lacking exon 16 as well as wild-type transcripts. Here, we tested an RNA-based antisense oligonucleotide (AON) strategy to correct the aberrant splicing caused by this mutation. Two AONs (AON1/2) complementary to the pre-mRNA IVS16 mutant region were designed and their effect on splicing was assessed at the RNA and protein levels, on intracellular protein localization and function. To this end, we used the 2657+5G>A mutant CFTR minigene stably expressed in HEK293 Flp-In cells that express a single copy of the transgene. RNA data from AON1-treated mutant cells show that exon 16 inclusion was almost completely restored (to 95%), also resulting in increased levels of correctly localized CFTR protein at the plasma membrane (PM) and with increased function. A novel two-color CFTR splicing reporter minigene developed here allowed the quantitative monitoring of splicing by automated microscopy localization of CFTR at the PM. The AON strategy is thus a promising therapeutic approach for the specific correction of alternative splicing.

  3. Method of predicting Splice Sites based on signal interactions

    Directory of Open Access Journals (Sweden)

    Deogun Jitender S

    2006-04-01

    Full Text Available Abstract Background Predicting and proper ranking of canonical splice sites (SSs is a challenging problem in bioinformatics and machine learning communities. Any progress in SSs recognition will lead to better understanding of splicing mechanism. We introduce several new approaches of combining a priori knowledge for improved SS detection. First, we design our new Bayesian SS sensor based on oligonucleotide counting. To further enhance prediction quality, we applied our new de novo motif detection tool MHMMotif to intronic ends and exons. We combine elements found with sensor information using Naive Bayesian Network, as implemented in our new tool SpliceScan. Results According to our tests, the Bayesian sensor outperforms the contemporary Maximum Entropy sensor for 5' SS detection. We report a number of putative Exonic (ESE and Intronic (ISE Splicing Enhancers found by MHMMotif tool. T-test statistics on mouse/rat intronic alignments indicates, that detected elements are on average more conserved as compared to other oligos, which supports our assumption of their functional importance. The tool has been shown to outperform the SpliceView, GeneSplicer, NNSplice, Genio and NetUTR tools for the test set of human genes. SpliceScan outperforms all contemporary ab initio gene structural prediction tools on the set of 5' UTR gene fragments. Conclusion Designed methods have many attractive properties, compared to existing approaches. Bayesian sensor, MHMMotif program and SpliceScan tools are freely available on our web site. Reviewers This article was reviewed by Manyuan Long, Arcady Mushegian and Mikhail Gelfand.

  4. Cultural Factors Related to Smoking in San Francisco's Irish Bars

    Science.gov (United States)

    Satterlund, Travis D.; Antin, Tamar M. J.; Lee, Juliet P.; Moore, Roland S.

    2009-01-01

    California's Smoke-Free Workplace Act was extended to include bars in 1998. While the majority of bars in the state have become smoke free, in many bars patrons and staff continue to smoke despite the law. The authors present findings from a study which assessed cultural factors related to continued smoking in bars in the city of San Francisco. In…

  5. Branching fractions for chi_cJ -> p p-bar pi^0, p p-bar eta, and p p-bar omega

    CERN Document Server

    Onyisi, P U E; Alexander, J P; Cassel, D G; Das, S; Ehrlich, R; Fields, L; Gibbons, L; Gray, S W; Hartill, D L; Heltsley, B K; Hunt, J M; Kreinick, D L; Kuznetsov, V E; Ledoux, J; Patterson, J R; Peterson, D; Riley, D; Ryd, A; Sadoff, A J; Shi, X; Sun, W M; Yelton, J; Rubin, P; Lowrey, N; Mehrabyan, S; Selen, M; Wiss, J; Adams, S; Kornicer, M; Mitchell, R E; Shepherd, M R; Tarbert, C M; Besson, D; Pedlar, T K; Xavier, J; Cronin-Hennessy, D; Hietala, J; Zweber, P; Dobbs, S; Metreveli, Z; Seth, K K; Xiao, T; Tomaradze, A; Brisbane, S; Libby, J; Martin, L; Powell, A; Spradlin, P; Wilkinson, G; Mendez, H; Ge, J Y; Miller, D H; Shipsey, I P J; Xin, B; Adams, G S; Hu, D; Moziak, B; Napolitano, J; Ecklund, K M; Insler, J; Muramatsu, H; Park, C S; Thorndike, E H; Yang, F; Ricciardi, S; Thomas, C; Artuso, M; Blusk, S; Mountain, R; Skwarnicki, T; Stone, S; Wang, J C; Zhang, L M; Bonvicini, G; Cinabro, D; Lincoln, A; Smith, M J; Zhou, P; Zhu, J; Naik, P; Rademacker, J; Asner, D M; Edwards, K W; Reed, J; Randrianarivony, K; Robichaud, A N; Tatishvili, G; White, E J; Briere, R A; Vogel, H

    2010-01-01

    Using a sample of 25.9 million psi(2S) decays acquired with the CLEO-c detector at the CESR e^+e^- collider, we report branching fractions for the decays chi_cJ -> p p-bar pi^0, p p-bar eta, and p p-bar omega, with J=0,1,2. Our results for B(chi_cJ-> p p-bar pi^0) and B(chi_cJ-> p p-bar eta) are consistent with, but more precise than, previous measurements. Furthermore, we include the first measurement of B(chi_cJ-> p p-bar omega).

  6. Evidence for the existence of $u d \\bar{b} \\bar{b}$ and the non-existence of $s s \\bar{b} \\bar{b}$ and $c c \\bar{b} \\bar{b}$ tetraquarks from lattice QCD

    CERN Document Server

    Bicudo, Pedro; Peters, Antje; Wagenbach, Björn; Wagner, Marc

    2015-01-01

    We combine lattice QCD results for the potential of two static antiquarks in the presence of two quarks $q q$ of finite mass and quark model techniques to study possibly existing $q q \\bar{b} \\bar{b}$ tetraquarks. While there is strong indication for a bound four-quark state for $q q = (ud-du) / \\sqrt{2}$, i.e. isospin $I=0$, we find clear evidence against the existence of corresponding tetraquarks with $q q \\in \\{ uu , (ud+du) / \\sqrt{2} , dd \\}$, i.e. isospin $I=1$, $q q = s s$ and $q q = c c$.

  7. Central Mass Concentration and Bar Dissolution in Nearby Spiral Galaxies

    CERN Document Server

    Das, M; Vogel, S N; Regan, M W; Sheth, K; Harris, A I; Jefferys, W H; Das, Mousumi; Teuben, Peter J.; Vogel, Stuart N.; Regan, Michael W.; Sheth, Kartik; Harris, Andrew I.; Jefferys, William H.

    2003-01-01

    We use data from the BIMA Survey of Nearby Galaxies (SONG) to investigate the relationship between ellipticity and central mass concentration in barred spirals. Existing simulations predict that bar ellipticity decreases as inflowing mass driven by the bar accumulates in the central regions, ultimately destroying the bar. Using the ratio of the bulge mass to the mass within the bar radius as an estimate of the central mass concentration, we obtain dynamical mass estimates from SONG CO 1-0 rotation curve data. We find an inverse correlation between bar ellipticity and central mass concentration, consistent with simulations of bar dissolution.

  8. Analysis of a Splice Array Experiment Elucidates Roles of Chromatin Elongation Factor Spt4-5 in Splicing.

    Directory of Open Access Journals (Sweden)

    2005-09-01

    Full Text Available Splicing is an important process for regulation of gene expression in eukaryotes, and it has important functional links to other steps of gene expression. Two examples of these linkages include Ceg1, a component of the mRNA capping enzyme, and the chromatin elongation factors Spt4-5, both of which have recently been shown to play a role in the normal splicing of several genes in the yeast Saccharomyces cerevisiae. Using a genomic approach to characterize the roles of Spt4-5 in splicing, we used splicing-sensitive DNA microarrays to identify specific sets of genes that are mis-spliced in ceg1, spt4, and spt5 mutants. In the context of a complex, nested, experimental design featuring 22 dye-swap array hybridizations, comprising both biological and technical replicates, we applied five appropriate statistical models for assessing differential expression between wild-type and the mutants. To refine selection of differential expression genes, we then used a robust model-synthesizing approach, Differential Expression via Distance Synthesis, to integrate all five models. The resultant list of differentially expressed genes was then further analyzed with regard to select attributes: we found that highly transcribed genes with long introns were most sensitive to spt mutations. QPCR confirmation of differential expression was established for the limited number of genes evaluated. In this paper, we showcase splicing array technology, as well as powerful, yet general, statistical methodology for assessing differential expression, in the context of a real, complex experimental design. Our results suggest that the Spt4-Spt5 complex may help coordinate splicing with transcription under conditions that present kinetic challenges to spliceosome assembly or function.

  9. Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing.

    Directory of Open Access Journals (Sweden)

    Yuanyuan Xiao

    2005-09-01

    Full Text Available Splicing is an important process for regulation of gene expression in eukaryotes, and it has important functional links to other steps of gene expression. Two examples of these linkages include Ceg1, a component of the mRNA capping enzyme, and the chromatin elongation factors Spt4-5, both of which have recently been shown to play a role in the normal splicing of several genes in the yeast Saccharomyces cerevisiae. Using a genomic approach to characterize the roles of Spt4-5 in splicing, we used splicing-sensitive DNA microarrays to identify specific sets of genes that are mis-spliced in ceg1, spt4, and spt5 mutants. In the context of a complex, nested, experimental design featuring 22 dye-swap array hybridizations, comprising both biological and technical replicates, we applied five appropriate statistical models for assessing differential expression between wild-type and the mutants. To refine selection of differential expression genes, we then used a robust model-synthesizing approach, Differential Expression via Distance Synthesis, to integrate all five models. The resultant list of differentially expressed genes was then further analyzed with regard to select attributes: we found that highly transcribed genes with long introns were most sensitive to spt mutations. QPCR confirmation of differential expression was established for the limited number of genes evaluated. In this paper, we showcase splicing array technology, as well as powerful, yet general, statistical methodology for assessing differential expression, in the context of a real, complex experimental design. Our results suggest that the Spt4-Spt5 complex may help coordinate splicing with transcription under conditions that present kinetic challenges to spliceosome assembly or function.

  10. Splign: algorithms for computing spliced alignments with identification of paralogs

    Directory of Open Access Journals (Sweden)

    Tatusova Tatiana

    2008-05-01

    Full Text Available Abstract Background The computation of accurate alignments of cDNA sequences against a genome is at the foundation of modern genome annotation pipelines. Several factors such as presence of paralogs, small exons, non-consensus splice signals, sequencing errors and polymorphic sites pose recognized difficulties to existing spliced alignment algorithms. Results We describe a set of algorithms behind a tool called Splign for computing cDNA-to-Genome alignments. The algorithms include a high-performance preliminary alignment, a compartment identification based on a formally defined model of adjacent duplicated regions, and a refined sequence alignment. In a series of tests, Splign has produced more accurate results than other tools commonly used to compute spliced alignments, in a reasonable amount of time. Conclusion Splign's ability to deal with various issues complicating the spliced alignment problem makes it a helpful tool in eukaryotic genome annotation processes and alternative splicing studies. Its performance is enough to align the largest currently available pools of cDNA data such as the human EST set on a moderate-sized computing cluster in a matter of hours. The duplications identification (compartmentization algorithm can be used independently in other areas such as the study of pseudogenes. Reviewers This article was reviewed by: Steven Salzberg, Arcady Mushegian and Andrey Mironov (nominated by Mikhail Gelfand.

  11. Two new splice variants in porcine PPARGC1A

    Directory of Open Access Journals (Sweden)

    Peelman Luc J

    2008-12-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptor γ coactivator 1α (PPARGC1A is a coactivator with a vital and central role in fat and energy metabolism. It is considered to be a candidate gene for meat quality in pigs and is involved in the development of obesity and diabetes in humans. How its many functions are regulated, is however still largely unclear. Therefore a transcription profile of PPARGC1A in 32 tissues and 4 embryonic developmental stages in the pig was constructed by screening its cDNA for possible splice variants with exon-spanning primers. Findings This led to the discovery of 2 new splice variants in the pig, which were subsequently also detected in human tissues. In these variants, exon 8 was either completely or partly (the last 66 bp were conserved spliced out, potentially coding for a much shorter protein of respectively 337 and 359 amino acids (aa, of which the first 291 aa would be the same compared to the complete protein (796 aa. Conclusion Considering the functional domains of the PPARGC1A protein, it is very likely these splice variants considerably affect the function of the protein and alternative splicing could be one of the mechanisms by which the diverse functions of PPARGC1A are regulated.

  12. RNA splicing in a new rhabdovirus from Culex mosquitoes.

    Science.gov (United States)

    Kuwata, Ryusei; Isawa, Haruhiko; Hoshino, Keita; Tsuda, Yoshio; Yanase, Tohru; Sasaki, Toshinori; Kobayashi, Mutsuo; Sawabe, Kyoko

    2011-07-01

    Among members of the order Mononegavirales, RNA splicing events have been found only in the family Bornaviridae. Here, we report that a new rhabdovirus isolated from the mosquito Culex tritaeniorhynchus replicates in the nuclei of infected cells and requires RNA splicing for viral mRNA maturation. The virus, designated Culex tritaeniorhynchus rhabdovirus (CTRV), shares a similar genome organization with other rhabdoviruses, except for the presence of a putative intron in the coding region for the L protein. Molecular phylogenetic studies indicated that CTRV belongs to the family Rhabdoviridae, but it is yet to be assigned a genus. Electron microscopic analysis revealed that the CTRV virion is extremely elongated, unlike virions of rhabdoviruses, which are generally bullet shaped. Northern hybridization confirmed that a large transcript (approximately 6,500 nucleotides [nt]) from the CTRV L gene was present in the infected cells. Strand-specific reverse transcription-PCR (RT-PCR) analyses identified the intron-exon boundaries and the 76-nt intron sequence, which contains the typical motif for eukaryotic spliceosomal intron-splice donor/acceptor sites (GU-AG), a predicted branch point, and a polypyrimidine tract. In situ hybridization exhibited that viral RNAs are primarily localized in the nucleus of infected cells, indicating that CTRV replicates in the nucleus and is allowed to utilize the host's nuclear splicing machinery. This is the first report of RNA splicing among the members of the family Rhabdoviridae.

  13. Oncogenes and RNA splicing of human tumor viruses.

    Science.gov (United States)

    Ajiro, Masahiko; Zheng, Zhi-Ming

    2014-09-01

    Approximately 10.8% of human cancers are associated with infection by an oncogenic virus. These viruses include human papillomavirus (HPV), Epstein-Barr virus (EBV), Merkel cell polyomavirus (MCV), human T-cell leukemia virus 1 (HTLV-1), Kaposi's sarcoma-associated herpesvirus (KSHV), hepatitis C virus (HCV) and hepatitis B virus (HBV). These oncogenic viruses, with the exception of HCV, require the host RNA splicing machinery in order to exercise their oncogenic activities, a strategy that allows the viruses to efficiently export and stabilize viral RNA and to produce spliced RNA isoforms from a bicistronic or polycistronic RNA transcript for efficient protein translation. Infection with a tumor virus affects the expression of host genes, including host RNA splicing factors, which play a key role in regulating viral RNA splicing of oncogene transcripts. A current prospective focus is to explore how alternative RNA splicing and the expression of viral oncogenes take place in a cell- or tissue-specific manner in virus-induced human carcinogenesis.

  14. Sec16 alternative splicing dynamically controls COPII transport efficiency.

    Science.gov (United States)

    Wilhelmi, Ilka; Kanski, Regina; Neumann, Alexander; Herdt, Olga; Hoff, Florian; Jacob, Ralf; Preußner, Marco; Heyd, Florian

    2016-08-05

    The transport of secretory proteins from the endoplasmic reticulum (ER) to the Golgi depends on COPII-coated vesicles. While the basic principles of the COPII machinery have been identified, it remains largely unknown how COPII transport is regulated to accommodate tissue- or activation-specific differences in cargo load and identity. Here we show that activation-induced alternative splicing of Sec16 controls adaptation of COPII transport to increased secretory cargo upon T-cell activation. Using splice-site blocking morpholinos and CRISPR/Cas9-mediated genome engineering, we show that the number of ER exit sites, COPII dynamics and transport efficiency depend on Sec16 alternative splicing. As the mechanistic basis, we suggest the C-terminal Sec16 domain to be a splicing-controlled protein interaction platform, with individual isoforms showing differential abilities to recruit COPII components. Our work connects the COPII pathway with alternative splicing, adding a new regulatory layer to protein secretion and its adaptation to changing cellular environments.

  15. Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica.

    Science.gov (United States)

    Kabran, Philomène; Rossignol, Tristan; Gaillardin, Claude; Nicaud, Jean-Marc; Neuvéglise, Cécile

    2012-06-01

    Alternative pre-mRNA splicing is a major mechanism contributing to the proteome complexity of most eukaryotes, especially mammals. In less complex organisms, such as yeasts, the numbers of genes that contain introns are low and cases of alternative splicing (AS) with functional implications are rare. We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica. The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism. This ubiquitous enzyme is involved in the tricarboxylic acid cycle in mitochondria and in the glyoxylate cycle, which takes place in peroxisomes and the cytosol. In Saccharomyces cerevisiae, three genes encode three compartment-specific enzymes. In contrast, only two genes exist in Y. lipolytica. One gene (YlMDH1, YALI0D16753g) encodes a predicted mitochondrial protein, whereas the second gene (YlMDH2, YALI0E14190g) generates the cytosolic and peroxisomal forms through the alternative use of two 3'-splice sites in the second intron. Both splicing variants were detected in cDNA libraries obtained from cells grown under different conditions. Mutants expressing the individual YlMdh2p isoforms tagged with fluorescent proteins confirmed that they localized to either the cytosolic or the peroxisomal compartment.

  16. The Vertical Stellar Kinematics in Face-On Barred Galaxies: Estimating the Ages of Bars

    CERN Document Server

    Gadotti, D A; Gadotti, Dimitri A.; Souza, Ronaldo E. de

    2005-01-01

    In order to perform a detailed study of the stellar kinematics in the vertical axis of bars, we obtained high signal-to-noise spectra along the major and minor axes of the bars in a sample of 14 face-on galaxies, and used them to determine the line of sight stellar velocity distribution, parameterized as Gauss-Hermite series. With these data, we developed a diagnostic tool that allows one to distinguish between recently formed and evolved bars, as well as estimate their ages, assuming that bars form in vertically thin disks, recognizable by low values for the vertical velocity dispersion sigma_z. Through N-body realizations of bar unstable disk galaxies we could also check the time scales involved in the processes which give bars an important vertical structure. We show that sigma_z in evolved bars is roughly around 100 Km/s, which translates to a height scale of about 1.4 Kpc, giving support to scenarios in which bulges form through disk material. Furthermore, the bars in our numerical simulations have value...

  17. Genome-wide identification of zero nucleotide recursive splicing in Drosophila.

    Science.gov (United States)

    Duff, Michael O; Olson, Sara; Wei, Xintao; Garrett, Sandra C; Osman, Ahmad; Bolisetty, Mohan; Plocik, Alex; Celniker, Susan E; Graveley, Brenton R

    2015-05-21

    Recursive splicing is a process in which large introns are removed in multiple steps by re-splicing at ratchet points--5' splice sites recreated after splicing. Recursive splicing was first identified in the Drosophila Ultrabithorax (Ubx) gene and only three additional Drosophila genes have since been experimentally shown to undergo recursive splicing. Here we identify 197 zero nucleotide exon ratchet points in 130 introns of 115 Drosophila genes from total RNA sequencing data generated from developmental time points, dissected tissues and cultured cells. The sequential nature of recursive splicing was confirmed by identification of lariat introns generated by splicing to and from the ratchet points. We also show that recursive splicing is a constitutive process, that depletion of U2AF inhibits recursive splicing, and that the sequence and function of ratchet points are evolutionarily conserved in Drosophila. Finally, we identify four recursively spliced human genes, one of which is also recursively spliced in Drosophila. Together, these results indicate that recursive splicing is commonly used in Drosophila, occurs in humans, and provides insight into the mechanisms by which some large introns are removed.

  18. Stellar diffusion in barred spiral galaxies

    CERN Document Server

    Brunetti, Maura; Pfenniger, Daniel

    2011-01-01

    We characterize empirically the radial diffusion of stars in the plane of a typical barred disk galaxy by calculating the local spatial diffusion coefficient and diffusion time-scale for bulge-disk-halo N-body self-consistent systems which initially differ in the Safronov-Toomre-Q_T parameter. We find different diffusion scenarios that depend on the bar strength and on the degree of instability of the disk. Marginally stable disks, with Q_T \\sim 1, have two families of bar orbits with different values of angular momentum and energy, which determine a large diffusion in the corotation region. In hot disks, Q_T> 1, stellar diffusion is reduced with respect to the case of marginally stable disks. In cold models, we find that spatial diffusion is not constant in time and strongly depends on the activity of the bar, which can move stars all over the disk recurrently. We conclude that to realistically study the impact of radial migration on the chemical evolution modeling of the Milky Way the role of the bar has to...

  19. $NDK$, $\\bar{K} DN$ and $ND\\bar{D}$ molecules

    CERN Document Server

    Xiao, C W; Oset, E

    2011-01-01

    We investigate theoretically baryon systems made of three hadrons which contain one nucleon and one D meson, and in addition another meson, $\\bar{D}, K$ or $\\bar{K}$. The systems are studied using the Fixed Center Approximation to the Faddeev equations. The study is made assuming scattering of a $K$ or a $\\bar{K}$ on a $DN$ cluster, which is known to generate the $\\Lambda_c(2595)$, or the scattering of a nucleon on the $D\\bar{D}$ cluster, which has been shown to generate a hidden charm resonance named X(3700). We also investigate the configuration of scattering of $N$ on the $KD$ cluster, which is known to generate the $D_{s0}^*(2317)$. In all cases we find bound states, with the $NDK$ system, of exotic nature, more bound than the $\\bar{K} DN$.

  20. Characterisation of vibration input to flywheel used on urban bus

    Science.gov (United States)

    Wang, L.; Kanarachos, S.; Christensen, J.

    2016-09-01

    Vibration induced from road surface has an impact on the durability and reliability of electrical and mechanical components attached on the vehicle. There is little research published relevant to the durability assessment of a flywheel energy recovery system installed on city and district buses. Relevant international standards and legislations were reviewed and large discrepancy was found among them, in addition, there are no standards exclusively developed for kinetic energy recovery systems on vehicles. This paper describes the experimentation of assessment of road surface vibration input to the flywheel on a bus as obtained at the MIRA Proving Ground. Power density spectra have been developed based on the raw data obtained during the experimentation. Validation of this model will be carried out using accelerated life time tests that will be carried out on a shaker rig using an accumulated profile based on the theory of fatigue damage equivalence in time and frequency domain aligned with the model predictions.