WorldWideScience

Sample records for bus bar splices

  1. Proposed Method for the Verification of the LHC Bus Bar Splices during Commissioning at Cryogenic Conditions

    CERN Document Server

    Calvi, M; Rodríguez-Mateos, F

    2007-01-01

    The commissioning of the Large Hadron Collider at CERN includes the powering of about 1600 superconducting electrical circuits to currents ranging from 55 A to 11.8 kA. A large number of splices (over 70'000) are present at the magnet interconnects, which can only be validated with current at cryogenic conditions. This paper discusses the thermal effects related to possible faulty splices during the powering of the circuits. The calculations of the quench and detection currents, as well as the hot spot temperatures, are described. The heat transfer model with the surrounding coolant and the current profiles inside the splices are presented. This study is completed with a sensitivity analysis on the hot spot temperature with respect to the model parameters. Finally, the implications with respect to the powering ramps and parameters to be applied during the first powering are discussed.

  2. A train for the bus(bars)

    CERN Multimedia

    CERN Bulletin

    2013-01-01

    On 8 April, the first SMACC (Superconducting Magnets and Circuits Consolidation) teams began work in the LHC tunnel. They are responsible for opening the interconnects between the magnets, laying the groundwork for the series of operations that will be performed by the team riding the ‘consolidation train’.   A technician installs the machine tool that allows them to prepare the surface of the section of the bar where the shunt will be fixed. The LHC’s 1,670 dipoles and quadrupoles are powered by power converters and connected by copper 'busbars’. The superconducting cables run through these bars, carrying a current of up to 11,850 amps. Six superconducting cables meet at each interconnect, where they are held together by a soldered (see box) electrical connection sandwiched between two pieces of copper, forming the splice between the busbars of the neighbouring magnets. The integrity of the electrical circuit is depen...

  3. Bus bar electrical feedthrough for electrorefiner system

    Science.gov (United States)

    Williamson, Mark; Wiedmeyer, Stanley G; Willit, James L; Barnes, Laurel A; Blaskovitz, Robert J

    2013-12-03

    A bus bar electrical feedthrough for an electrorefiner system may include a retaining plate, electrical isolator, and/or contact block. The retaining plate may include a central opening. The electrical isolator may include a top portion, a base portion, and a slot extending through the top and base portions. The top portion of the electrical isolator may be configured to extend through the central opening of the retaining plate. The contact block may include an upper section, a lower section, and a ridge separating the upper and lower sections. The upper section of the contact block may be configured to extend through the slot of the electrical isolator and the central opening of the retaining plate. Accordingly, relatively high electrical currents may be transferred into a glovebox or hot-cell facility at a relatively low cost and higher amperage capacity without sacrificing atmosphere integrity.

  4. DWPF Melter No.2 Prototype Bus Bar Test Report

    International Nuclear Information System (INIS)

    Gordon, J.

    2003-01-01

    Characterization and performance testing of a prototype DWPF Melter No.2 Dome Heater Bus Bar are described. The prototype bus bar was designed to address the design features of the existing system which may have contributed to water leaks on Melter No.1. Performance testing of the prototype revealed significant improvement over the existing design in reduction of both bus bar and heater connection maximum temperature, while characterization revealed a few minor design and manufacturing flaws in the bar. The prototype is recommended as an improvement over the existing design. Recommendations are also made in the area of quality control to ensure that critical design requirements are met

  5. Black silicon solar cells with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by maskless reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Four different methods to obtain blackened bus-bar strings were compared with respect to reflectance, and two of these methods (i.e., oxidized copper and etched solder) were used to fabricate functional allblack solar 9-cell panels. The black bus-bars (e.......g., by oxidized copper) have a reflectance below 3% in the entire visible wavelength range. The combination of black silicon cells and blackened bus-bars results in aesthetic, all-black panels based on conventional, front-contacted solar cells without compromising efficiency....

  6. Black silicon with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by mask-less reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Black bus-bars were realized by oxidized copper resulting in reflectance below 3% in the entire visible wavelength range. The combination of these two technologies may result in aesthetic, all-black panels based on conventional, front-contacted solar cells...

  7. Bus bar joints stability and protection

    CERN Document Server

    Verweij, A

    2009-01-01

    A defective bus connection between two dipole magnets was the primary cause of the incident in sector 34 on September 19th. I will show how this could have happened, i.e. how a highly resistive joint has caused a thermal runaway and burned (or opened) before the QPS threshold was reached. In the second part of the talk I will present the new detection limits for the QPS upgrade of the RB and RQ circuits, required to avoid similar thermal runaways in the future.

  8. The high current bus-bars of the LHC from conception to manufacture

    CERN Document Server

    Belova, L; Perinet-Marquet, J-L; Urpin, C

    2006-01-01

    The main magnets of the LHC are series-connected electrically in different excitation circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper section all along length. The function of the copper section is essentially to provide an alternative path for the magnet current in case the superconducting cable loses its superconductivity and returns to normal state because of a transient system disturbance or normal zone propagation coming from the neighboring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100 ° C while the copper is conducting. With regard to that, a quench signal is initiated, which in turn triggers the ramping down of the current from 13000 A to 0....

  9. Calculation of Flexible Bus-Bars Electrodynamic Stability with Application of Implicit Scheme

    Directory of Open Access Journals (Sweden)

    Y. G. Panamarenka

    2008-01-01

    Full Text Available A numerical method for calculation of open-air substations’ flexible bus-bars dynamic at short-circuit has been improved on equations of a flexible elastic string with application of an implicit scheme. On the basis of the numerical method a computer program FLEBUS for calculation of substations’ flexible bus-bars dynamic at short-circuit has been developed. An approbation and an estimation of calculation result reliability have been carried out in accordance with the program while using experimental data. On the basis of the obtained information it is possible to assert that the developed program is an independent tool for calculation of electrodynamic stability of substations’ flexible bus-bars.

  10. Design and Manufacture of the Superconducting Bus-bars for the LHC Main Magnets

    CERN Document Server

    Belova, L M; Perinet-Marquet, J L; Ivanov, P; Urpin, C

    2002-01-01

    The main magnets of the LHC are series-connected electrically in different powering circuits by means of superconducting bus-bars, carrying a maximum current of 13 kA. These superconducting bus-bars consist of a superconducting cable thermally and electrically coupled to a copper profile all along the length. The function of the copper profile is essentially to provide an alternative path for the current in case the superconducting cable loses its superconducting state and returns to normal state because of a transient disturbance or of a normal zone propagation coming from the neighbouring magnets. When a superconducting bus-bar quenches to normal state its temperature must always stay below a safe values of about 100°C while the copper is conducting. When a resistive transition is detected, the protection systems triggers the ramping down of the current from 13000 A to 0. The ramp rate must not exceed a maximum value to avoid the transition of magnets series-connected in the circuit. This paper concerns th...

  11. Mechanical splicing of superelastic Cu–Al–Mn alloy bars with headed ends

    Science.gov (United States)

    Kise, S.; Mohebbi, A.; Saiidi, M. S.; Omori, T.; Kainuma, R.; Shrestha, K. C.; Araki, Y.

    2018-06-01

    This paper examines the feasibility of mechanical splicing using a steel coupler to connect headed ends of superelastic Cu–Al–Mn alloy (Camalloy) bars and steel reinforcing bars to be used in concrete structures. Although threading of Camalloy is as easy as that of steel, mechanical splicing using threaded ends requires machining of Camalloy bars into dog-bone shape to avoid brittle fracture at the threaded ends. The machining process requires significant time and cost and wastes substantial amount of the material. This paper attempts to resolve this issue by applying mechanical splicing using steel couplers to connect headed ends of Camalloy and steel reinforcing bars. To study its feasibility, we prepare 3 specimens wherein both ends of each Camalloy bar (13 mm diameter and 300 mm length) are connected to steel reinforcing bars. The specimens are tested under monotonic, single-cycle, and full-cycle tension loading conditions. From these tests, we observed (1) excellent superelasticity with recoverable strain of around 6% and (2) large ductility with fracture strain of over 19%. It should be emphasized here that, in all the specimens, ductile fracture occurred at the locations apart from the headed ends. This is in sharp contrast with brittle fracture of headed superelastic Ni–Ti SMA bars, most of which took place around the headed ends. From the results of the microstructural analysis, we identified the following reasons for avoiding brittle fracture at the headed ends: (1) Precipitation hardening increases the strength around the boundary between the straight and headed (tapered) portions, where stress concentration takes place. (2) The strength of the straight portion does not increase significantly up to the ductile fracture if its grain orientation is close to 〈0 0 1〉.

  12. Thermo-electric Analysis of the Interconnection of the LHC main Superconducting Bus Bars

    CERN Document Server

    Granieri, P P; Casali, M; Bottura, L; Siemko, A

    2013-01-01

    Spurred by the question of the maximum allowable energy for the operation of the Large Hadron Collider (LHC), we have progressed in the understanding of the thermo-electric behavior of the 13 kA superconducting bus bars interconnecting its main magnets. A deep insight of the underlying mechanisms is required to ensure the protection of the accelerator against undesired effects of resistive transitions. This is especially important in case of defective interconnections which can jeopardize the operation of the whole LHC. In this paper we present a numerical model of the interconnections between the main dipole and quadrupole magnets, validated against experimental tests of an interconnection sample with a purposely built-in defect. We consider defective interconnections featuring a lack of bonding among the superconducting cables and the copper stabilizer components, such as those that could be present in the machine. We evaluate the critical defect length limiting the maximum allowable current for powering th...

  13. Analysis of Defective Interconnections of the 13 kA LHC Superconducting Bus Bars

    CERN Document Server

    Granieri, P P; Bianchi, M; Breschi, M; Bottura, L; Willering, G

    2012-01-01

    The interconnections between Large Hadron Collider (LHC) main dipole and quadrupole magnets are made of soldered joints of two superconducting cables stabilized by a copper bus bar. The 2008 incident revealed the possible presence of defects in the interconnections of the 13 kA circuits that could lead to unprotected resistive transitions. Since then thorough experimental and numerical investigations were undertaken to determine the safe operating conditions for the LHC. This paper reports the analysis of experimental tests reproducing defective interconnections between main quadrupole magnets. A thermo-electromagnetic model was developed taking into account the complicated sample geometry. Close attention was paid to the physical description of the heat transfer towards helium, one of the main unknown parameters. The simulation results are reported in comparison with the measurements in case of static He I cooling bath. The outcome of this study constitutes a useful input to improve the stability assessment ...

  14. Thermal Performance of the LHC External Auxiliary Bus-Bar Tube Mathematical Modelling

    CERN Document Server

    Kowalczyk, P; Sacré, P; Skoczen, Blazej

    1998-01-01

    The Large Hadron Collider (LHC) externally routed auxiliary bus-bar tube (EAB) will house the electrical feeders of the LHC short straight section (SSS) correcting magnets. The superconducting wires w ill be contained in a stainless steel tube and immersed in a quasi-static helium bath. The EAB thermal performance during the cooling of the magnets down to the operating temperature of 1.9 K is studi ed. A 3-d finite element thermal model of the EAB during a cooling process from 293 K to 4.5 K is described. The semi-analytical model of the EAB cool-down from 4.5 K to 1.9 K is also presented.

  15. Thermo-electric analysis of the interconnection of the LHC main superconducting bus bars

    Science.gov (United States)

    Granieri, P. P.; Breschi, M.; Casali, M.; Bottura, L.; Siemko, A.

    2013-01-01

    Spurred by the question of the maximum allowable energy for the operation of the Large Hadron Collider (LHC), we have progressed in the understanding of the thermo-electric behavior of the 13 kA superconducting bus bars interconnecting its main magnets. A deep insight of the underlying mechanisms is required to ensure the protection of the accelerator against undesired effects of resistive transitions. This is especially important in case of defective interconnections which can jeopardize the operation of the whole LHC. In this paper we present a numerical model of the interconnections between the main dipole and quadrupole magnets, validated against experimental tests of an interconnection sample with a purposely built-in defect. We consider defective interconnections featuring a lack of bonding among the superconducting cables and the copper stabilizer components, such as those that could be present in the machine. We evaluate the critical defect length limiting the maximum allowable current for powering the magnets. We determine the dependence of the critical defect length on different parameters as the heat transfer towards the cooling helium bath, the quality of manufacturing, the operating conditions and the protection system parameters, and discuss the relevant mechanisms.

  16. High-Current Bus Splice Resistances and Implications for the Operating Energy of the LHC

    CERN Document Server

    Koratzinos, M; Charifoulline, Z; Dahlerup-Petersen, K; Denz, R; Flora, R H; Pfeffer, H; Scheuerlein, C; Schmidt, R; Siemko, A; Strait, J; Verweij, A

    2010-01-01

    At each interconnection between LHC main magnets a low-resistance solder joint must be made between superconducting cables in order to provide a continuous current path through the superconductor and also to the surrounding copper stabilizer in case the cable quenches [1]. About 10,000 such joints exist in the LHC. An extensive campaign has been undertaken to characterize and map the resistances of these joints. All of the superconducting cable splices were measured at 1.9 K and no splices were found with a resistance larger than 3 nW. Non-invasive measurements of the stabilizer joints were made at 300 K in 5 of the 8 sectors, and at 80 K in 3 sectors. More precise local measurements were made on suspect interconnects that were opened up, and poor joints were repaired. However, it is likely that additional imperfect stabilizer joints still exist in the LHC. A statistical analysis is used to place bounds on the remaining worst-case resistances. This sets limits on the maximum operating energy of the LHC, prior...

  17. Heat generation by eddy currents in a shell of superconducting bus-bars for SIS100 particle accelerator at FAIR

    Directory of Open Access Journals (Sweden)

    Tomków Łukasz

    2017-12-01

    Full Text Available Superconducting magnets in the SIS100 particle accelerator require the supply of liquid helium and electric current. Both are transported with by-pass lines designed at Wrocław University of Technology. Bus-bars used to transfer an electric current between the sections of the accelerator will be encased in a steel shell. Eddy currents are expected to appear in the shell during fast-ramp operation of magnets. Heat generation, which should be limited in any cryogenic system, will appear in the shell. In this work the amount of heat generated is assessed depending on the geometry of an assembly of the bus-bars and the shell. Numerical and analytical calculations are described. It was found that heat generation in the shell is relatively small when compared to other sources present in the accelerator and its value strongly depends on the geometry of the shell. The distribution of eddy currents and generated heat for different geometrical options are presented. Based on the results of the calculations the optimal design is proposed.

  18. Measurement of the Residual Resistivity Ratio of the Bus Bars Copper Stabilizer of the 13 kA Circuits of the LHC

    CERN Document Server

    Apollonio, A; Solfaroli Camillocci, M; Schmidt, R; Verweij, A; Siemko, A; Claudet, S; Steckert, J; Thiesen, H

    2012-01-01

    After the incident of September 2008, the operational beam energy of the LHC has been set to 3.5 TeV, since not all joints of the superconducting (SC) bus bars between magnets have the required quality for 7 TeV operation. This decision is based on simulations to determine the safe current in the main dipole and quadrupole circuits, reproducing the thermal behaviour of a quenched superconducting joint by taking into account all relevant factors that affect a possible thermal runaway. One important parameter is the Residual Resistivity Ratio (RRR) of the copper stabilizer of the bus bar connecting the superconducting magnets. A dedicated campaign to measure the RRR for the main 13 kA circuits of the LHC in all sectors was performed during the Christmas stop in December 2010 and January 2011. The measurement method as well as the data analysis and results are presented in this paper.

  19. Copper Heat Exchanger for the External Auxiliary Bus-Bars Routing Line in the LHC Insertion Regions

    CERN Document Server

    Garion, C; Seyvet, F; Sitko, M; Skoczen, B; Tock, J P

    2006-01-01

    The corrector magnets and the main quadrupoles of the LHC dispersion suppressors are powered by a special superconducting line (called auxiliary bus-bars line N), external to the cold mass and housed in a 50 mm diameter stainless steel tube fixed to the cold mass. As the line is periodically connected to the cold mass, the same gaseous and liquid helium cools both the magnets and the line. The final sub-cooling process (from around 4.5 K down to 1.9 K) consists in the phase transformation from liquid to superfluid helium. Heat is extracted from the line through the magnets via their point of junction. In dispersion suppressor zones, approximately 40 m long, the sub-cooling of the line is slightly delayed with respect to the magnets. This might have an impact on the readiness of the accelerator for operation. In order to accelerate the process, a special heat exchanger has been designed. It is located in the middle of the dispersion suppressor portion of the line. Its main function consists in providing a loca...

  20. The LHC SSS cold mass inside the cryostat. The complexity of the bus-bars for the power supply of the magnets and cryogenic links can be seen. The two apertures in the centre will house the beam lines

    CERN Document Server

    2003-01-01

    The LHC SSS cold mass inside the cryostat. The complexity of the bus-bars for the power supply of the magnets and cryogenic links can be seen. The two apertures in the centre will house the beam lines

  1. Bus basics

    International Nuclear Information System (INIS)

    Dobinson, R.W.

    1983-01-01

    The aims of this course are: 1. To introduce the general concepts of buses used to interconnect processors, perpherals, and instrumentation. 2. To classify, albeit crudely, different types of bus systems. 3. To give some examples of past, present, and future buses as applied to the field of high-energy physics, including some details of software. 4. To provide references for further study. (orig.)

  2. Superconducting link bus design for the accelerator project for upgrade of LHC

    International Nuclear Information System (INIS)

    Nobrega, F.; Brandt, J.; Cheban, S.; Feher, S.; Kaducak, M.; Kashikhin, V.; Peterson, T.

    2011-01-01

    The Accelerator Project for Upgrade of LHC (APUL) is a U.S. project participating in and contributing to CERN's Large Hadron Collider (LHC) upgrade program. Fermi National Accelerator Laboratory in collaboration with Brookhaven National Laboratory was developing sub-systems for the upgrade of the LHC final focus magnet systems. Part of the upgrade called for various lengths of superconducting power transmission lines known as SC Links which were up to 100 m long. The SC Link electrically connects the current leads in the Distribution Feed Boxes to the interaction region magnets. The SC Link is an extension of the magnet bus housed within a cryostat. The present concept for the bus consists of 22 power cables, 4 x 13 kA, 2 x 7 kA, 8 x 2.5 kA and 8 x 0.6 kA bundled into one bus. Different cable and strand possibilities were considered for the bus design including Rutherford cable. The Rutherford cable bus design potentially would have required splices at each sharp elbow in the SC Link. The advantage of the round bus design is that splices are only required at each end of the bus during installation at CERN. The round bus is very flexible and is suitable for pulling through the cryostat. Development of the round bus prototype and of 2 splice designs is described in this paper. Magnetic analysis and mechanical test results of the 13 kA cable and splices are presented.

  3. ELECTRO-THERMAL AND MECHANICAL VALIDATION EXPERIMENT ON THE LHC MAIN BUSBAR SPLICE CONSOLIDATION

    CERN Document Server

    Willering, GP; Bourcey, N; Bottura, L; Charrondiere, M; Cerqueira Bastos, M; Deferne, G; Dib, G; Giloux, Chr; Grand-Clement, L; Heck, S; Hudson, G; Kudryavtsev, D; Perret, P; Pozzobon, M; Prin, H; Scheuerlein, Chr; Rijllart, A; Triquet, S; Verweij, AP

    2012-01-01

    To eliminate the risk of thermal runaways in LHC interconnections a consolidation by placing shunts on the main bus bar interconnections is proposed by the Task Force Splices Consolidation. To validate the design two special SSS magnet spares are placed on a test bench in SM-18 to measure the interconnection in between with conditions as close as possible to the LHC conditions. Two dipole interconnections are instrumented and prepared with worst-case-conditions to study the thermo-electric stability limits. Two quadrupole interconnections are instrumented and prepared for studying the effect of current cycling on the mechanical stability of the consolidation design. All 4 shunted interconnections showed very stable behaviour, well beyond the LHC design current cycle.

  4. Effect of tension lap splice on the behavior of high strength concrete (HSC beams

    Directory of Open Access Journals (Sweden)

    Ahmed El-Azab

    2014-12-01

    Full Text Available In the recent years, many research efforts have been carried out on the bond strength between normal strength concrete (NSC and reinforcing bars spliced in tension zones in beams. Many codes gave a minimum splice length for tension and compression reinforcement as a factor of the bar diameter depending on many parameters such as concrete strength, steel yield stress, shape of bar end, shape of bar surface and also bar location. Also, codes gave another restriction about the percentage of total reinforcement to be spliced at the same time. Comparatively limited attention has been directed toward the bond between high strength concrete (HSC and reinforcing bars spliced in tension zones in beams. HSC has high modulus of elasticity, high density and long-term durability. This research presents an experimental study on the bond between high strength concrete (HSC and reinforcing bars spliced in tension zones in beams. It reports the influence of several parameters on bond in splices. The parameters covered are casting position, splice length as a factor of bar diameter, bar diameter and reinforcement ratio. The research involved tests on sixteen simply-supported beams of 1800 mm span, 200 mm width and 400 mm thickness made of HSC. In each beam, the total tensile steel bars were spliced in the constant moment zone. Crack pattern, crack propagation, cracking load, failure load and mi span deflection were recorded and analyzed to study the mentioned parameters effect.

  5. GO Bus impact analysis.

    Science.gov (United States)

    2013-08-01

    NJ TRANSIT launched two relatively new enhanced bus services: GO 25 serving the : Springfield Avenue corridor in 2008 and GO 28 serving the Bloomfield Avenue corridor in 2009. : As an enhanced but not full Bus Rapid Transit (BRT) service, GO Bus feat...

  6. Intelligent Bus Stops in the Flexible Bus Systems

    OpenAIRE

    Razi Iqbal; Muhammad Usman Ghani

    2014-01-01

    The purpose of this paper is to discuss Intelligent Bus Stops in a special Demand Responsive Transit (DRT), the Flexible Bus System. These Intelligent Bus Stops are more efficient and information rich than Traditional Bus Stops. The real time synchronization of the Flexible Bus System makes it unique as compared to Traditional Bus Systems. The Main concern is to make Bus Stops intelligent and information rich. Buses are informed about the no. of passengers waiting at the upcoming ...

  7. Flexural behavior of concrete beam with mechanical splices of reinforcement subjected to cyclic loading

    International Nuclear Information System (INIS)

    Nab, H. S.; Kim, W. B.

    2008-01-01

    In nuclear power plant structures, the mechanical rebar splices are designated and constructed on the basis of ACI and ASME code. Regardless of good performance on mechanical rebar splices, these splicing methods that did not be registered on ASME code have not restricted to apply to construction site. In this study, the main candidate splice is cold roll formed parallel threaded splice. This was registered newly in ASME Section III division 2 CC 4333 'Mechanical Splices' in 2004. To compare the traditional rebar splice with mechanical rebar splices, concrete beams were made to evaluate the ductility of spliced reinforcing bars. Based on Experimental results, it was identified that the mechanical rebar splices by parallel threaded coupler had better accumulated dissipation energy capacity to resist seismic behavior than the traditional lapping splices. It showed that concrete specimens with D36 reinforcing bar coupler are 1.8 times better performance and that concrete specimens with D22 reinforcing bar coupler are 2.8 times better performance. (authors)

  8. Estimating bus passenger waiting times from incomplete bus arrivals data

    OpenAIRE

    McLeod, F.N.

    2007-01-01

    This paper considers the problem of estimating bus passenger waiting times at bus stops using incomplete bus arrivals data. This is of importance to bus operators and regulators as passenger waiting time is a key performance measure. Average waiting times are usually estimated from bus headways, that is, time gaps between buses. It is both time-consuming and expensive to measure bus arrival times manually so methods using automatic vehicle location systems are attractive; however, these syste...

  9. Intelligent Bus Stops in the Flexible Bus Systems

    Directory of Open Access Journals (Sweden)

    Razi Iqbal

    2014-09-01

    Full Text Available The purpose of this paper is to discuss Intelligent Bus Stops in a special Demand Responsive Transit (DRT, the Flexible Bus System. These Intelligent Bus Stops are more efficient and information rich than Traditional Bus Stops. The real time synchronization of the Flexible Bus System makes it unique as compared to Traditional Bus Systems. The Main concern is to make Bus Stops intelligent and information rich. Buses are informed about the no. of passengers waiting at the upcoming Bus Stops. If there are no passengers to ride or get off on upcoming Bus Stop, the Bus can skip that Bus Stop and head towards the next Bus Stop where passenger is waiting, which will decrease the ride time of the passengers on the Bus and also the wait time of the passengers waiting on the upcoming Bus Stops. Providing more information at Bus Stops about the Destination (Time to Destination, Distance to Destination etc. and Buses (Bus Location, Arrival Time of Bus etc. makes it easier for the passengers to decide whether to ride a particular Bus or not.

  10. TPG bus passes

    CERN Multimedia

    Staff Association

    2013-01-01

    The CERN Staff Association will stop selling TPG bus passes. All active and retired members of the CERN personnel will be able to purchase Unireso bus passes from the CERN Hostel - Building 39 (Meyrin site) from 1st February 2013. For more information: https://cds.cern.ch/journal/CERNBulletin/2013/04/Announcements/1505279?ln=en

  11. Targeting Splicing in Prostate Cancer

    OpenAIRE

    Effrosyni Antonopoulou; Michael Ladomery

    2018-01-01

    Over 95% of human genes are alternatively spliced, expressing splice isoforms that often exhibit antagonistic functions. We describe genes whose alternative splicing has been linked to prostate cancer; namely VEGFA, KLF6, BCL2L2, ERG, and AR. We discuss opportunities to develop novel therapies that target specific splice isoforms, or that target the machinery of splicing. Therapeutic approaches include the development of small molecule inhibitors of splice factor kinases, splice isoform speci...

  12. The erigone bus study

    International Nuclear Information System (INIS)

    Durieu, L.; Vignes, M.; Wolstenholme, P.

    1982-01-01

    A study has been made of some factors determining the performance and cost-effectiveness of microcomputer bus systems implemented in Europa crates. An experimental system has been specified and a number of modules constructed in accordance with the specification, in order to test the validity of some of the ideas proposed. It has been demonstrated that the bus interfacing logic can be simple and inexpensive, while maintaining a high degree of processor independence and also a high degree of transparency in operation. The number of pins required by the bus is minimized by multiplexing address and data. A series termination for the drive of the multiplexed lines permits cross-talk to be reduced to insignificant levels: this technique is recommended for wider application. The experimental bus is proposed as a basis for the construction of simple microcomputer-based instrumentation systems. (orig.)

  13. Creative Bus Financing.

    Science.gov (United States)

    Malone, Wade

    1982-01-01

    Alternative ways of financing school bus purchases include financing privately through contractors or commercial banks, financing through sources such as insurance companies and pension funds, leasing the buses, or contracting for transportation services. (Author/MLF)

  14. SPFC bus design studies

    Energy Technology Data Exchange (ETDEWEB)

    Potter, L.; Reinkingh, J.

    1999-07-01

    This report presents the results of a study assessing the design options for a solid polymer fuel cell bus. Commercial and operation requirements, environmental and market drivers, and fuel processor modeling are examined. Power train specifications and detailed system design are investigated covering fuel cell system dynamic response, hybrid system size, fuel cell system start-up time, system specifications, and hybrid bus component dimensions and costs. (UK)

  15. Savannah River bus project

    Energy Technology Data Exchange (ETDEWEB)

    Summers, W.A. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1998-08-01

    The H2Fuel Bus is the world`s first hybrid hydrogen electric transit bus. It was developed through a public/private partnership involving several leading technology and industrial organizations in the Southeast, with primary funding and program management provided by the Department of Energy. The primary goals of the project are to gain valuable information on the technical readiness and economic viability of hydrogen buses and to enhance the public awareness and acceptance of emerging hydrogen technologies. The bus has been operated by the transit agency in Augusta, Georgia since April, 1997. It employs a hybrid IC engine/battery/electric drive system, with onboard hydrogen fuel storage based on the use of metal hydrides. Initial operating results have demonstrated an overall energy efficiency (miles per Btu) of twice that of a similar diesel-fueled bus and an operating range twice that of an all-battery powered electric bus. Tailpipe emissions are negligible, with NOx less than 0.2 ppm. Permitting, liability and insurance issues were addressed on the basis of extensive risk assessment and safety analyses, with the inherent safety characteristic of metal hydride storage playing a major role in minimizing these concerns. Future plans for the bus include continued transit operation and use as a national testbed, with potential modifications to demonstrate other hydrogen technologies, including fuel cells.

  16. LHC on the bus

    CERN Multimedia

    Laëtitia Pedroso

    2010-01-01

    On 15 December, an airport bus will be transformed in the image of CERN. The bus will be seen by the thousands of travellers arriving in Geneva, informing them of the possibility to visit CERN.   Sketch of the bus. The good relationship between Geneva International Airport and CERN started several years ago. In 2004 the airport put advertising space in the arrivals area at CERN's disposal free of charge. Now, starting on 15 December, a 40-foot long bus will display a giant sticker advertisement depicting CERN as it takes passengers over the airport tarmac to their planes. This is no ordinary sticker, and it was no mean task to attach it to the bus. The task of producing and attaching it was entrusted to Geneva-based specialists Mathys SA. With the ski season opening on 15 December, there will be many travellers arriving at the airport, and the bus will be ready to receive them. When one thinks of CERN, the subjects that naturally come to mind are the LHC, the mysteries of the Universe...

  17. PyBus -- A Python Software Bus

    OpenAIRE

    Lavrijsen, W

    2005-01-01

    A software bus, just like its hardware equivalent, allows for the discovery, installation, configuration, loading, unloading, and run-time replacement of software components, as well as channeling of inter-component communication. Python, a popular open-source programming language, encourages a modular design on software written in it, but it offers little or no component functionality. However, the language and its interpreter provide sufficient hooks to implement a thin, integral layer...

  18. Bus fuel consumption model

    Energy Technology Data Exchange (ETDEWEB)

    Zargari, S.A. [Iran Univ. of Science and Technology, Teheran (Iran, Islamic Republic of); Khan, A.M. [Carleton Univ., Ottawa, ON (Canada). Dept. of Civil and Environmental Engineering

    2000-07-01

    The interest in rapid bus transit has increased sharply with the realization that modern metropolitan areas rely on public transit to provide for strong economies and communities. As a prevention tool against traffic congestion, deteriorating air quality and rising greenhouse gas emissions, this study of bus fuel consumption was designed to assist in the planning and management of rapid bus transit. The Australian Road Research Board's (ARRB) Road Fuel Consumption Model was used as a starting point. The estimations required were realized with the help of Newtonian Mechanics. The four states of vehicular traffic were examined: acceleration, cruise, deceleration, and idle. The estimated total power required from the engine to overcome resistance forces, to run vehicle accessories and overcome internal engine friction was calculated. The data for the standard and articulated bus was obtained from OC Transpo in Ottawa. The study permitted the authors to conclude that the estimations for the parameters for power requirements and fuel consumption for heavy duty vehicles are appropriate. The methodology for the estimation of fuel consumption on the Transitway, which is part of the rapid bus transit system, proved adequate. In addition, the methodology was useful to estimate fuel savings resulting from demand management strategies with potential for modal shift. 9 refs., 6 tabs.

  19. Mechanical rebar splicing

    Directory of Open Access Journals (Sweden)

    Milosavljević Branko

    2014-01-01

    Full Text Available Different mechanical rebar splicing systems are presented, and design situations where mechanical splicing has advantage over reinforcement splicing by overlapping and welding are defined in this paper. New international standards for testing and proof of systems for mechanical rebar splicing quality are considered. Mechanical splicing system for rebar and bolt connection, usable in steel and reinforced concrete structural elements connections, is presented in this paper. There are only few examples of mechanical rebar splicing in our country. The most significant one - the pylon and beam connection at Ada Bridge in Belgrade is presented in the paper. Intensive development of production and use of mechanical rebar splicing systems, research in this area, as well as the publication of international standards prescribing requirements for quality and procedures for proof of quality, represent very good base for development of the corresponding technical norms in Serbia. The legislation in this area would quicken proof of quality procedures, attest and approval issuing for individual products, leading to wider use of this system in all situations where it is in advantage over the classical reinforcement splicing.

  20. spliceR

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Porse, Bo Torben; Sandelin, Albin

    2014-01-01

    RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternative splicing...

  1. Learning NServiceBus

    CERN Document Server

    Boike, David

    2013-01-01

    This is a practical tutorial containing hands-on examples for creating a messaging and SOA based service bus.This book is for .NET developers who are looking for ways to overcome problems related to buggy third party web service integrations, codebases that have grown into a big ball of mud, and batch jobs failure.

  2. Optical Fiber Fusion Splicing

    CERN Document Server

    Yablon, Andrew D

    2005-01-01

    This book is an up-to-date treatment of optical fiber fusion splicing incorporating all the recent innovations in the field. It provides a toolbox of general strategies and specific techniques that the reader can apply when optimizing fusion splices between novel fibers. It specifically addresses considerations important for fusion splicing of contemporary specialty fibers including dispersion compensating fiber, erbium-doped gain fiber, polarization maintaining fiber, and microstructured fiber. Finally, it discusses the future of optical fiber fusion splicing including silica and non-silica based optical fibers as well as the trend toward increasing automation. Whilst serving as a self-contained reference work, abundant citations from the technical literature will enable readers to readily locate primary sources.

  3. Performance of Grouted Splice Sleeve Connector under Tensile Load

    Directory of Open Access Journals (Sweden)

    A. Alias

    2016-05-01

    Full Text Available The grouted splice sleeve connector system takes advantage of the bond-slip resistance of the grout and the mechanical gripping of reinforcement bars to provide resistance to tensile force. In this system, grout acts as a load-transferring medium and bonding material between the bars and sleeve. This study adopted the end-to-end rebars connection method to investigate the effect of development length and sleeve diameter on the bonding performance of the sleeve connector. The end-to-end method refers to the condition where reinforcement bars are inserted into the sleeve from both ends and meet at the centre before grout is filled. Eight specimens of grouted splice sleeve connector were tested under tensile load to determine their performance. The sleeve connector was designed using 5 mm thick circular hollow section (CHS steel pipe and consisted of one external and two internal sleeves. The tensile test results show that connectors with a smaller external and internal sleeve diameter appear to provide better bonding performance. Three types of failure were observed in this research, which are bar fracture (outside the sleeve, bar pullout, and internal sleeve pullout. With reference to these failure types, the development length of 200 mm is the optimum value due to its bar fracture type, which indicates that the tensile capacity of the connector is higher than the reinforcement bar. It is found that the performance of the grouted splice sleeve connector is influenced by the development length of the reinforcement bar and the diameter of the sleeve.

  4. Circuit breaker and bus

    International Nuclear Information System (INIS)

    Vogel, H.F.

    1978-01-01

    Two close-open operations are required each pulse cycle at approximately 3 MA with the breaker in series with the burn supply, both forming a leg in parallel with the E-coil and the homopolar. To conduct the 3-MA current, approximately 30 breaker units are connected in parallel. Each unit is equipped with a counterpulse capacitor bank, and the bus for the 30 units is provided with a common saturable reactor core made of strip steel wound around the bus. Oil breakers of a special design are chosen because of their high arc resistance facilitating the current transfer to the homopolar machine and for obtaining precision timing in opening and closing of the contacts. The precision timing is achieved by a shock wave method with the oil serving as hydraulic medium. Each of the parallel breaker units possesses two sets of contacts, a stationary and a transient one. A 1-mΩ resistor in series with the transient contacts of each breaker unit is to improve the current sharing. The bus consists of room temperature copper, stranded and transposed to help provide current equalization under transient conditions

  5. Effect of confinement on bond strength of hot-dip galvanized lap splices in concrete structures

    International Nuclear Information System (INIS)

    Fakhran, Mazen

    2004-01-01

    Galvanizing the reinforcing steel is one of the methods used to protect bars against corrosion. Galvanizing is a hot dip process where the reinforcing bars are immersed in an aqueous pre flux solution of zinc ammonium chloride at a controlled temperature between 840 and 850 degrees F. In 2001, a research program was started at AUB to evaluate experimentally the effect of hot dip galvanizing on the bond capacity of tension lap splices anchored in full-scale beam specimens designed to fail in bond splitting mode. The test results indicated that the use of galvanized bars had a negligible effect on bond strength of reinforcement in normal strength. However, galvanizing caused an average of 20 percent decrease in bond strength of reinforcement in high strength concrete. The primary objective of research reported in this thesis, is the need to find a solution to eliminate the bond reduction of galvanized bars in high strength concrete. It is significant to evaluate the positive effect of the addition of transverse reinforcement in the splice region. The hypothesis to be tested is that such transverse reinforcement will insure uniform bond stress distribution over the entire splice region, thus mobilizing all bar lugs along the splice in the stress transfer mechanism between the bar and the surrounding concrete. Such mechanism might reduce the significant decrease in bond strength in high strength concrete due to galvanizing. To achieve this objective, eighteen full-scale beam specimens were tested in positive bending. Each beam was reinforced with bars spliced in a constant moment region at midspam. The splice length was chosen in such a way that the beams failed in bond splitting of the concrete cover in the splice region. The main variables were type of coating (black or galvanized bars), bar size (20, 25 and 32 mm), and amount of transverse reinforcement in the splice region (0, 2 or 4 stirrups). The test results indicated that confinement did not have a significant

  6. Miniature JPL Universal Instrument Bus

    Data.gov (United States)

    National Aeronautics and Space Administration — Develop a Universal Digital Processor Bus architecture using state of the art commercial packaging technologies. This work will transition commercial advanced- yet...

  7. The neurogenetics of alternative splicing.

    Science.gov (United States)

    Vuong, Celine K; Black, Douglas L; Zheng, Sika

    2016-05-01

    Alternative precursor-mRNA splicing is a key mechanism for regulating gene expression in mammals and is controlled by specialized RNA-binding proteins. The misregulation of splicing is implicated in multiple neurological disorders. We describe recent mouse genetic studies of alternative splicing that reveal its critical role in both neuronal development and the function of mature neurons. We discuss the challenges in understanding the extensive genetic programmes controlled by proteins that regulate splicing, both during development and in the adult brain.

  8. Learning NServiceBus Sagas

    CERN Document Server

    Helton, Rich

    2015-01-01

    If you are an Enterprise C# developer who wishes to extend your knowledge of NServiceBus and Enterprise Service Bus in C#, this is the book for you. This book is designed to enhance the education of ESBs and their messaging, whether you are a beginner or a seasoned expert in Enterprise C#, Apex, and Visualforce pages.

  9. Analysis for Behavior of Reinforcement Lap Splices in Deep Beams

    Directory of Open Access Journals (Sweden)

    Ammar Yaser Ali

    2018-03-01

    Full Text Available The present study includes an experimental and theoretical investigation of reinforced concrete deep beams containing tensile reinforcement lap splices at constant moment zone under static load. The study included two stages: in the first one, an experimental work included testing of eight simply supported RC deep beams having a total length (L = 2000 mm, overall depth (h= 600 mm and width (b = 150 mm. The tested specimens were divided into three groups to study the effect of main variables: lap length, location of splice, internal confinement (stirrups and external confinement (strengthening by CFRP laminates. The experimental results showed that the use of CFRP as external strengthening in deep beam with lap spliced gives best behavior such as increase in stiffness, decrease in deflection, delaying the cracks appearance and reducing the crack width. The reduction in deflection about (14-21 % than the unstrengthened beam and about (5-14 % than the beam with continuous bars near ultimate load. Also, it was observed that the beams with unstrengthened tensile reinforcement lap splices had three types of cracks: flexural, flexural-shear and splitting cracks while the beams with strengthened tensile reinforcement lap splices or continuous bars don’t observe splitting cracks. In the second stage, a numerical analysis of three dimensional finite element analysis was utilized to explore the behavior of the RC deep beams with tensile reinforcement lap splices, in addition to parametric study of many variables. The comparison between the experimental and theoretical results showed reasonable agreement. The average difference of the deflection at service load was less than 5%.

  10. Natural gas powered bus

    International Nuclear Information System (INIS)

    Ambuehl, D.; Fernandez, J.

    2003-01-01

    This report for the Swiss Federal Office of Energy presents the results of a project carried out by the Swiss Federal Institute of Technology in Zurich to evaluate the performance of a natural-gas-powered bus in comparison with two diesel buses. The report provides details on the vehicles, their routes and the results of interviews made with both passengers and drivers. Details of measurements made on fuel consumption and pollutant emissions are presented in tabular and graphical form, as are those made on noise emissions inside and outside the vehicles. The conclusions of the project are presented including economic aspects of using gas as a motor fuel. Also, the views of passengers, who were more concerned with comfort aspects, and drivers, who were more interested in technical aspects, are quoted

  11. Hybrid Electric Transit Bus

    Science.gov (United States)

    Viterna, Larry A.

    1997-01-01

    A government, industry, and university cooperative is developing an advanced hybrid electric city transit bus. Goals of this effort include doubling the fuel economy compared to current buses and reducing emissions to one-tenth of current EPA standards. Unique aspects of the vehicle's power system include the use of ultra-capacitors as an energy storage system, and a planned natural gas fueled turbogenerator developed from a small jet engine. Power from both the generator and energy storage system is provided to a variable speed electric motor attached to the rear axle. At over 15000 kg gross weight, this is the largest vehicle of its kind ever built using ultra-capacitor energy storage. This paper describes the overall power system architecture, the evolution of the control strategy, and its performance over industry standard drive cycles.

  12. 76 FR 37184 - Discretionary Bus and Bus Facilities Program

    Science.gov (United States)

    2011-06-24

    ... transporting bicycles on public transportation vehicles. The FY 2011 Appropriations Act allows a 90 percent Federal share for the total cost of a biodiesel bus. The Act also allows a 90 percent Federal share for...

  13. Air-pollution and economics: diesel bus versus electric bus

    OpenAIRE

    Adheesh, SR; Vasisht, Shravanth M; Ramasesha, Sheela K

    2016-01-01

    The Bangalore Metropolitan Transport Corporation (BMTC) took an initiative to check the overall benefits of introducing electric buses as a suitable replacement for the diesel buses to tackle the burgeoning pollution in the city of Bengaluru, India. For a trial run of three months, an electric bus was procured from a Chinese company `Build Your Dreams' (BYD). Data were collected by BMTC on the operation and maintenance of the bus. This new initiative, if rightly guided, could have a direct im...

  14. Alternative REST Splicing Underappreciated

    OpenAIRE

    Chen, Guo-Lin; Miller, Gregory

    2017-01-01

    As a major orchestrator of the cellular epigenome, the repressor element-1 silencing transcription factor (REST) can either repress or activate thousands of genes depending on cellular context, suggesting a highly context-dependent REST function tuned by environmental cues. While REST shows cell-type non-selective active transcription, an N-terminal REST4 isoform caused by alternative splicing - inclusion of an extra exon (N3c) which introduces a pre-mature stop codon - has been implicated in...

  15. A design of high-precision BLDCM drive with bus voltage protection

    Science.gov (United States)

    Lian, Xuezheng; Wang, Haitao; Xie, Meilin; Huang, Wei; Li, Dawei; Jing, Feng

    2017-11-01

    In the application of space satellite turntable, the design of balance wheel is very necessary. To solve the acquisition precision of Brushless DC motor speed is low, and the encoder is also more complex, this paper improves the original hall signal measurement methods. Using the logic device to achieve the six frequency multiplication of hall signal, the signal is used as speed feedback to achieve speed closed-loop control and improve the speed stability. At the same time, in order to prevent the E.M.F of BLDC motor to raise the voltage of the bus bar when reversing or braking, and affect the normal operation of other circuit modules, the analog circuit is used to protect the bus bar voltage by the way of energy consumption braking. The experimental results are consistent with the theoretical design, and the rationality and feasibility of the frequency multiplication scheme and bus voltage protection scheme are verified.

  16. Bar dimensions and bar shapes in estuaries

    Science.gov (United States)

    Leuven, Jasper; Kleinhans, Maarten; Weisscher, Steven; van der Vegt, Maarten

    2016-04-01

    Estuaries cause fascinating patterns of dynamic channels and shoals. Intertidal sandbars are valuable habitats, whilst channels provide access to harbors. We still lack a full explanation and classification scheme for the shapes and dimensions of bar patterns in natural estuaries, in contrast with bars in rivers. Analytical physics-based models suggest that bar length in estuaries increases with flow velocity, tidal excursion length or estuary width, depending on which model. However, these hypotheses were never validated for lack of data and experiments. We present a large dataset and determine the controls on bar shape and dimensions in estuaries, spanning bar lengths from centimeters (experiments) to 10s of kilometers length. First, we visually identified and classified 190 bars, measured their dimensions (width, length, height) and local braiding index. Data on estuarine geometry and tidal characteristics were obtained from governmental databases and literature on case studies. We found that many complex bars can be seen as simple elongated bars partly cut by mutually evasive ebb- and flood-dominated channels. Data analysis shows that bar dimensions scale with estuary dimensions, in particular estuary width. Breaking up the complex bars in simple bars greatly reduced scatter. Analytical bar theory overpredicts bar dimensions by an order of magnitude in case of small estuarine systems. Likewise, braiding index depends on local width-to-depth ratio, as was previously found for river systems. Our results suggest that estuary dimensions determine the order of magnitude of bar dimensions, while tidal characteristics modify this. We will continue to model bars numerically and experimentally. Our dataset on tidal bars enables future studies on the sedimentary architecture of geologically complex tidal deposits and enables studying effects of man-induced perturbations such as dredging and dumping on bar and channel patterns and habitats.

  17. IMPACTS OF BUS STOP IMPROVEMENTS

    Science.gov (United States)

    2018-03-23

    Improving bus stops by providing shelters, seating, signage, and sidewalks is relatively inexpensive and popular among riders and local officials. Making such improvements, however, is not often a priority for U.S. transit providers because of compet...

  18. Scaling Laws in Chennai Bus Network

    OpenAIRE

    Chatterjee, Atanu; Ramadurai, Gitakrishnan

    2015-01-01

    In this paper, we study the structural properties of the complex bus network of Chennai. We formulate this extensive network structure by identifying each bus stop as a node, and a bus which stops at any two adjacent bus stops as an edge connecting the nodes. Rigorous statistical analysis of this data shows that the Chennai bus network displays small-world properties and a scale-free degree distribution with the power-law exponent, $\\gamma > 3$.

  19. Alternative RNA splicing and cancer

    Science.gov (United States)

    Liu, Sali; Cheng, Chonghui

    2015-01-01

    Alternative splicing of pre-messenger RNA (mRNA) is a fundamental mechanism by which a gene can give rise to multiple distinct mRNA transcripts, yielding protein isoforms with different, even opposing, functions. With the recognition that alternative splicing occurs in nearly all human genes, its relationship with cancer-associated pathways has emerged as a rapidly growing field. In this review, we summarize recent findings that have implicated the critical role of alternative splicing in cancer and discuss current understandings of the mechanisms underlying dysregulated alternative splicing in cancer cells. PMID:23765697

  20. Mutual interdependence of splicing and transcription elongation.

    Science.gov (United States)

    Brzyżek, Grzegorz; Świeżewski, Szymon

    2015-01-01

    Transcription and splicing are intrinsically linked, as splicing needs a pre-mRNA substrate to commence. The more nuanced view is that the rate of transcription contributes to splicing regulation. On the other hand there is accumulating evidence that splicing has an active role in controlling transcription elongation by DNA-dependent RNA polymerase II (RNAP II). We briefly review those mechanisms and propose a unifying model where splicing controls transcription elongation to provide an optimal timing for successive rounds of splicing.

  1. Bus systems in motor vehicles; Bussysteme im Kfz

    Energy Technology Data Exchange (ETDEWEB)

    Schule, Roland

    2008-07-01

    There is hardly any modern vehicle without a bus system. This interactive learning software explains why electric and electronic systems in motor vehicles should be linked and uses various bus types to illustrate the various alternatives, bus structures, and data types. The physical side of bus systems is gone into, the data structure is explained, and the various bus typologies are outlined. From the fundamentals of bus systems, the software proceeds to present the most important bus systems and their main properties. Subjects: Diagnosis bus, LIN bus, CAN bus, MOST bus, Bluetooth. The bus systems EIA-485, LVDS, D{sup 2}B, byteflight, and Flexray are briefly characterised. (orig.)

  2. Fuel cell bus operation, system investigation H2 bus

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    The WP covers two tasks: - Prepartion of Technical Catalogue: In cooperation with ICIL, AR have compiled a technical catalogue, providing the impartial descriptions, both of existing technology and regulations, and the likely future developments of these, as to remedy the first problem faced by a potential hydrogen bus fleet operator viz the absence of an impartial description of the available vehicle and fuels systems together with the absence of a description of regulatory and safety factors which need consideration. - Fuel Cell Bus Operation - System Investigation H 2 Bus: The application of fuel cell electric generation systems to hybrid electrical buses or electrical busses without any storage system on board is considered. The task will cover safety and environmental aspects, a cost estimate and a market evaluation. (orig.)

  3. San Antonio's 1983 Bus Passenger Survey

    Science.gov (United States)

    1983-10-01

    Many transit operators routinely collect ridership data in order to provide a : basis for the planning of bus services. Often a survey is conducted of bus : riders to determine their riding habits, their views about the quality of : current service, ...

  4. School Bus Accidents and Driver Age.

    Science.gov (United States)

    McMichael, Judith

    The study examines the rates and types of school bus accidents according to the age of the school bus driver. Accident rates in North Carolina for the school year 1971-72 were analyzed using three sources of data: accident reports, driver and mileage data, and questionnaires administered to a sample of school bus drivers. Data were obtained on…

  5. Optimizing Tailored Bus Bridging Paths

    NARCIS (Netherlands)

    Gu, Wei; Yu, Jie; Ji, Yuxiong; van der Gun, J.P.T.; Pel, A.J.; Zhang, H. Michael; van Arem, B.

    2017-01-01

    Metro disruptions due to unexpected events reduce transit system reliability, resulting in significant productivity loss and long passenger delays. Bus bridging strategy is often used to connect stations affected by metro disruptions such that passengers could continue their journey. The literature

  6. Bus transport in Greater Manchester

    DEFF Research Database (Denmark)

    Sørensen, Claus Hedegaard; Gudmundsson, Henrik

    component regards the organisation of public bus transport at the local level. This involves the deregulation and privatisation reforms introduced by the Conservative Government from 1986 onwards, as well as the ‘partnerships’ and other new instruments introduced by the Labour Government in the Transport...

  7. Pybus - A Python Software Bus

    International Nuclear Information System (INIS)

    Lavrijsen, Wim T.L.P.

    2004-01-01

    A software bus, just like its hardware equivalent, allows for the discovery, installation, configuration, loading, unloading, and run-time replacement of software components, as well as channeling of inter-component communication. Python, a popular open-source programming language, encourages a modular design on software written in it, but it offers little or no component functionality. However, the language and its interpreter provide sufficient hooks to implement a thin, integral layer of component support. This functionality can be presented to the developer in the form of a module, making it very easy to use. This paper describes a Pythonmodule, PyBus, with which the concept of a ''software bus'' can be realized in Python. It demonstrates, within the context of the ATLAS software framework Athena, how PyBus can be used for the installation and (run-time) configuration of software, not necessarily Python modules, from a Python application in a way that is transparent to the end-user

  8. Handbook of knotting and splicing

    CERN Document Server

    Hasluck, Paul N

    2005-01-01

    Clearly written and amply illustrated with 208 figures, this classic guide ranges from simple and useful knots to complex varieties. Additional topics include rope splicing, working cordage, hammock making, more.

  9. Analysis of robustness of urban bus network

    Science.gov (United States)

    Tao, Ren; Yi-Fan, Wang; Miao-Miao, Liu; Yan-Jie, Xu

    2016-02-01

    In this paper, the invulnerability and cascade failures are discussed for the urban bus network. Firstly, three static models(bus stop network, bus transfer network, and bus line network) are used to analyse the structure and invulnerability of urban bus network in order to understand the features of bus network comprehensively. Secondly, a new way is proposed to study the invulnerability of urban bus network by modelling two layered networks, i.e., the bus stop-line network and the bus line-transfer network and then the interactions between different models are analysed. Finally, by modelling a new layered network which can reflect the dynamic passenger flows, the cascade failures are discussed. Then a new load redistribution method is proposed to study the robustness of dynamic traffic. In this paper, the bus network of Shenyang City which is one of the biggest cities in China, is taken as a simulation example. In addition, some suggestions are given to improve the urban bus network and provide emergency strategies when traffic congestion occurs according to the numerical simulation results. Project supported by the National Natural Science Foundation of China (Grant Nos. 61473073, 61374178, 61104074, and 61203329), the Fundamental Research Funds for the Central Universities (Grant Nos. N130417006, L1517004), and the Program for Liaoning Excellent Talents in University (Grant No. LJQ2014028).

  10. Barred Owl [ds8

    Data.gov (United States)

    California Natural Resource Agency — These data define the current range of Barred and hybrid Barred/Spotted Owls in California. The current range includes the coastal mountains of northern California...

  11. Electrical system architecture having high voltage bus

    Science.gov (United States)

    Hoff, Brian Douglas [East Peoria, IL; Akasam, Sivaprasad [Peoria, IL

    2011-03-22

    An electrical system architecture is disclosed. The architecture has a power source configured to generate a first power, and a first bus configured to receive the first power from the power source. The architecture also has a converter configured to receive the first power from the first bus and convert the first power to a second power, wherein a voltage of the second power is greater than a voltage of the first power, and a second bus configured to receive the second power from the converter. The architecture further has a power storage device configured to receive the second power from the second bus and deliver the second power to the second bus, a propulsion motor configured to receive the second power from the second bus, and an accessory motor configured to receive the second power from the second bus.

  12. Observations of barred spirals

    International Nuclear Information System (INIS)

    Elmegreen, D.M.

    1990-01-01

    Observations of barred spiral galaxies are discussed which show that the presence of a bar increases the likelihood for grand design spiral structure only in early Hubble types. This result is contrary to the more common notion that grand design spiral structure generally accompanies bars in galaxies. Enhanced deprojected color images are shown which reveal that a secondary set of spiral arms commonly occurs in barred galaxies and also occasionally in ovally distorted galaxies. 6 refs

  13. Methods to detect faulty splices in the superconducting magnet system of the LHC

    International Nuclear Information System (INIS)

    Bailey, R.; Bellesia, B.; Lasheras, N.Catalan; Dahlerup-Petersen, K.; Denz, R.; Robles, C.; Koratzinos, M.; Pojer, M.; Ponce, L.; Saban, R.; Schmidt, R.

    2009-01-01

    The incident of 19 September 2008 at the LHC was caused by a faulty inter-magnet splice of about 200 n(Omega) resistance. Cryogenic and electrical techniques have been developed to detect other abnormal splices, either between or inside the magnets. The existing quench protection system can be used to detect internal splices with R > 20 n(Omega). Since this system does not cover the bus between magnets, the cryogenic system is used to measure the rate of temperature rise due to ohmic heating. Accuracy of a few mK/h, corresponding to a few Watts, has been achieved, allowing detection of excess resistance, if it is more than 40 n(Omega) in a cryogenic subsector (two optical cells). Follow-up electrical measurements are made in regions identified by the cryogenic system. These techniques have detected two abnormal internal magnet splices of 100 n(Omega) and 50 n(Omega) respectively. In 2009, this ad hoc system will be replaced with a permanent one to monitor all splices at the n(Omega) level

  14. Methods to detect faulty splices in the superconducting magnet system of the LHC

    CERN Document Server

    Bailey, R; Catalan Lasheras, N; Dahlerup-Petersen, K; Denz, R; Robles, C; Koratzinos, M; Pojer, M; Ponce, L; Saban, R; Schmidt, R; Siemko, A; Solfaroli Camillocci, M; Thiesen, H; Vergara Fernandez, A; Flora, R H; Charifoulline, Z; Bednarek, M; Górnicki, E; Jurkiewicz, P; Kapusta, P; Strait, J

    2010-01-01

    The incident of 19 September 2008 at the LHC was caused by a faulty inter-magnet splice of about 200 nΩ resistance. Cryogenic and electrical techniques have been developed to detect other abnormal splices, either between or inside the magnets. The existing quench protection system can be used to detect internal splices with R>20 nΩ. Since this system does not cover the bus between magnets, the cryogenic system is used to measure the rate of temperature rise due to ohmic heating. Accuracy of a few mK/h, corresponding to a few Watts, has been achieved, allowing detection of excess resistance, if it is more than 40 nΩ in a cryogenic subsector (two optical cells). Follow-up electrical measurements are made in regions identified by the cryogenic system. These techniques have detected two abnormal internal magnet splices of 100 nΩ and 50 nΩ respectively. In 2009, this ad hoc system will be replaced with a permanent one to monitor all splices at the nΩ level.

  15. On Modified Bar recursion

    DEFF Research Database (Denmark)

    Oliva, Paulo Borges

    2002-01-01

    Modified bar recursion is a variant of Spector's bar recursion which can be used to give a realizability interpretation of the classical axiom of dependent choice. This realizability allows for the extraction of witnesses from proofs of forall-exists-formulas in classical analysis. In this talk I...... shall report on results regarding the relationship between modified and Spector's bar recursion. I shall also show that a seemingly weak form of modified bar recursion is as strong as "full" modified bar recursion in higher types....

  16. Avionic Data Bus Integration Technology

    Science.gov (United States)

    1991-12-01

    address the hardware-software interaction between a digital data bus and an avionic system. Very Large Scale Integration (VLSI) ICs and multiversion ...the SCP. In 1984, the Sperry Corporation developed a fault tolerant system which employed multiversion programming, voting, and monitoring for error... MULTIVERSION PROGRAMMING. N-version programming. 226 N-VERSION PROGRAMMING. The independent coding of a number, N, of redundant computer programs that

  17. New TPG bus route 28

    CERN Multimedia

    2003-01-01

    Geneva's Public Transport services (TPG) have recently inaugurated a new bus line 28, connecting the La Tour Hospital in Meyrin to the international organisations in Geneva, via the airport. All signs associated with this route will be yellow in colour. Timetables and route details can be found at http://www.tpg.ch. Relations with the Host States Service http://www.cern.ch/relations/ Tel. 72848

  18. Probability of burn-through of defective 13 kA splices at increased energy levels

    CERN Document Server

    Verweij, A

    2011-01-01

    In many 13 kA splices in the machine there is a lack of bonding between the superconducting cable and the stabilising copper along with a bad contact between the bus stabiliser and the splice stabiliser. In case of a quench of such a defective splice, the current cannot bypass the cable through the copper, hence leading to excessive local heating of the cable. This may result in a thermal runaway and burn-through of the cable in a time smaller than the time constant of the circuit. Since it is not possible to protect against this fast thermal run-away, one has to limit the current to a level that is small enough so that a burn-through cannot occur. Prompt quenching of the joint, and quenching due to heat propagation through the bus and through the helium are considered. Probabilities for joint burn-through are given for the RB circuit for beam energies of 3.5, 4 and 4.5 TeV, and a decay time constant of the RB circuit of 50 and 68 s.

  19. National fuel cell bus program : proterra fuel cell hybrid bus report, Columbia demonstration.

    Science.gov (United States)

    2011-10-01

    This report summarizes the experience and early results from a fuel cell bus demonstration funded by the Federal Transit Administration (FTA) under the National Fuel Cell Bus Program. A team led by the Center for Transportation and the Environment an...

  20. Bus Route Design with a Bayesian Network Analysis of Bus Service Revenues

    OpenAIRE

    Liu, Yi; Jia, Yuanhua; Feng, Xuesong; Wu, Jiang

    2018-01-01

    A Bayesian network is used to estimate revenues of bus services in consideration of the effect of bus travel demands, passenger transport distances, and so on. In this research, the area X in Beijing has been selected as the study area because of its relatively high bus travel demand and, on the contrary, unsatisfactory bus services. It is suggested that the proposed Bayesian network approach is able to rationally predict the probabilities of different revenues of various route services, from...

  1. Bar and Theta Hyperoperations

    Directory of Open Access Journals (Sweden)

    Thomas Vougiouklis

    2011-12-01

    Full Text Available In questionnaires the replacement of the scale of Likert by a bar was suggested in 2008 by Vougiouklis & Vougiouklis. The use of the bar was rapidly accepted in social sciences. The bar is closely related with fuzzy theory and has several advantages during both the filling-in questionnaires and mainly in the research processing. In this paper we relate hyperstructure theory with questionnaires and we study the obtained hyperstructures which are used as an organising device of the problem.

  2. Human Splicing Finder: an online bioinformatics tool to predict splicing signals

    OpenAIRE

    Desmet, Francois-Olivier; Hamroun, Dalil; Lalande, Marine; Collod-Beroud, Gwenaelle; Claustres, Mireille; Beroud, Christophe

    2009-01-01

    International audience; Thousands of mutations are identified yearly. Although many directly affect protein expression, an increasing proportion of mutations is now believed to influence mRNA splicing. They mostly affect existing splice sites, but synonymous, non-synonymous or nonsense mutations can also create or disrupt splice sites or auxiliary cis-splicing sequences. To facilitate the analysis of the different mutations, we designed Human Splicing Finder (HSF), a tool to predict the effec...

  3. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs

    Science.gov (United States)

    Zhang, Xiao-Ou; Dong, Rui; Zhang, Yang; Zhang, Jia-Lin; Luo, Zheng; Zhang, Jun; Chen, Ling-Ling; Yang, Li

    2016-01-01

    Circular RNAs (circRNAs) derived from back-spliced exons have been widely identified as being co-expressed with their linear counterparts. A single gene locus can produce multiple circRNAs through alternative back-splice site selection and/or alternative splice site selection; however, a detailed map of alternative back-splicing/splicing in circRNAs is lacking. Here, with the upgraded CIRCexplorer2 pipeline, we systematically annotated different types of alternative back-splicing and alternative splicing events in circRNAs from various cell lines. Compared with their linear cognate RNAs, circRNAs exhibited distinct patterns of alternative back-splicing and alternative splicing. Alternative back-splice site selection was correlated with the competition of putative RNA pairs across introns that bracket alternative back-splice sites. In addition, all four basic types of alternative splicing that have been identified in the (linear) mRNA process were found within circRNAs, and many exons were predominantly spliced in circRNAs. Unexpectedly, thousands of previously unannotated exons were detected in circRNAs from the examined cell lines. Although these novel exons had similar splice site strength, they were much less conserved than known exons in sequences. Finally, both alternative back-splicing and circRNA-predominant alternative splicing were highly diverse among the examined cell lines. All of the identified alternative back-splicing and alternative splicing in circRNAs are available in the CIRCpedia database (http://www.picb.ac.cn/rnomics/circpedia). Collectively, the annotation of alternative back-splicing and alternative splicing in circRNAs provides a valuable resource for depicting the complexity of circRNA biogenesis and for studying the potential functions of circRNAs in different cells. PMID:27365365

  4. Road safety issues for bus transport management.

    Science.gov (United States)

    Cafiso, Salvatore; Di Graziano, Alessandro; Pappalardo, Giuseppina

    2013-11-01

    Because of the low percentage of crashes involving buses and the assumption that public transport improves road safety by reducing vehicular traffic, public interest in bus safety is not as great as that in the safety of other types of vehicles. It is possible that less attention is paid to the significance of crashes involving buses because the safety level of bus systems is considered to be adequate. The purpose of this study was to evaluate the knowledge and perceptions of bus managers with respect to safety issues and the potential effectiveness of various technologies in achieving higher safety standards. Bus managers were asked to give their opinions on safety issues related to drivers (training, skills, performance evaluation and behaviour), vehicles (maintenance and advanced devices) and roads (road and traffic safety issues) in response to a research survey. Kendall's algorithm was used to evaluate the level of concordance. The results showed that the majority of the proposed items were considered to have great potential for improving bus safety. The data indicated that in the experience of the participants, passenger unloading and pedestrians crossing near bus stops are the most dangerous actions with respect to vulnerable users. The final results of the investigation showed that start inhibition, automatic door opening, and the materials and internal architecture of buses were considered the items most strongly related to bus passenger safety. Brake assistance and vehicle monitoring systems were also considered to be very effective. With the exception of driver assistance systems for passenger and pedestrian safety, the perceptions of the importance of other driver assistance systems for vehicle monitoring and bus safety were not unanimous among the bus company managers who participated in this survey. The study results showed that the introduction of new technologies is perceived as an important factor in improving bus safety, but a better understanding

  5. American fuel cell bus project : first analysis report.

    Science.gov (United States)

    2013-06-01

    This report summarizes the experience and early results from the American Fuel Cell Bus Project, a fuel cell electric bus demonstration : funded by the Federal Transit Administration (FTA) under the National Fuel Cell Bus Program. A team led by CALST...

  6. Fuel Cell Electric Bus Evaluations | Hydrogen and Fuel Cells | NREL

    Science.gov (United States)

    Bus Evaluations Fuel Cell Electric Bus Evaluations NREL's technology validation team evaluates fuel cell electric buses (FCEBs) to provide comprehensive, unbiased evaluation results of fuel cell bus early transportation applications for fuel cell technology. Buses operate in congested areas where

  7. Design & Development of FPGA Based VME Bus Controller

    OpenAIRE

    Himali Patel; Poornima Talwai

    2015-01-01

    The VME bus interface Controller (VIC068A) is used to interface Local CPU bus and VME bus. VME Bus Controller is used in wide application areas where high reliability, good accuracy and high speed are desired to withstand industrial environment like nuclear power plant and process industries. VME Bus controller can configure as Master, Slave, Interrupt Handler, arbiter as well as power monitor. Commercial VME Bus interface controller chips are available from a few vend...

  8. GC content around splice sites affects splicing through pre-mRNA secondary structures

    Directory of Open Access Journals (Sweden)

    Chen Liang

    2011-01-01

    Full Text Available Abstract Background Alternative splicing increases protein diversity by generating multiple transcript isoforms from a single gene through different combinations of exons or through different selections of splice sites. It has been reported that RNA secondary structures are involved in alternative splicing. Here we perform a genomic study of RNA secondary structures around splice sites in humans (Homo sapiens, mice (Mus musculus, fruit flies (Drosophila melanogaster, and nematodes (Caenorhabditis elegans to further investigate this phenomenon. Results We observe that GC content around splice sites is closely associated with the splice site usage in multiple species. RNA secondary structure is the possible explanation, because the structural stability difference among alternative splice sites, constitutive splice sites, and skipped splice sites can be explained by the GC content difference. Alternative splice sites tend to be GC-enriched and exhibit more stable RNA secondary structures in all of the considered species. In humans and mice, splice sites of first exons and long exons tend to be GC-enriched and hence form more stable structures, indicating the special role of RNA secondary structures in promoter proximal splicing events and the splicing of long exons. In addition, GC-enriched exon-intron junctions tend to be overrepresented in tissue-specific alternative splice sites, indicating the functional consequence of the GC effect. Compared with regions far from splice sites and decoy splice sites, real splice sites are GC-enriched. We also found that the GC-content effect is much stronger than the nucleotide-order effect to form stable secondary structures. Conclusion All of these results indicate that GC content is related to splice site usage and it may mediate the splicing process through RNA secondary structures.

  9. Mastering NServiceBus and persistence

    CERN Document Server

    Helton, Rich

    2014-01-01

    This book is intended for developers, designers, and architects alike who wish to build C# NServiceBus enterprise architectures and learn how ESB persists data and messages to help them attain their goals. No prior knowledge of persistence in NServiceBus is required.

  10. Digital processing data communication systems (bus systems)

    International Nuclear Information System (INIS)

    Fleck, K.

    1980-01-01

    After an introduction to the technology of digital processing data communication systems there are the following chapters: digital communication of processing data in automation technology, the technology of biserial communication, the implementaiton of a bus system, the data transmission of the TDC-2000 system of Honeywell's and the process bus CS 275 in the automation system TELEPERM M of Siemens AG. (WB) [de

  11. Hanging off a bar

    NARCIS (Netherlands)

    Mueller, F.; Walmink, W.; Toprak, C.; Bongers, Bert; Graether, E.; Hoven, van den E.A.W.H.

    2012-01-01

    Exertion Games involve physical effort and as a result can facilitate physical health benefits. We present Hanging off a Bar, an action hero-inspired Exertion Game in which players hang off an exercise bar over a virtual river for as long as possible. Initial observations from three events with

  12. Raising the bar (6)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, Maria; Amaral, Pedro; Bhattacharjee, Arnab; Corrado, Luisa; Doran, Justin; Fingleton, Bernard; Fuerst, Franz; Garretsen, Harry; Igliori, Danilo; Le Gallo, Julie; McCann, Philip; Monastiriotis, Vassilis; Quatraro, Francesco; Yu, Jihai

    2017-01-01

    Raising the bar (6). Spatial Economic Analysis. This editorial summarizes and comments on the papers published in issue 12(4) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper addresses the question of whether 'jobs follow people' or 'people follow

  13. Bar-tailed

    NARCIS (Netherlands)

    Duijns, S.; Hidayati, N.A.; Piersma, T.

    2013-01-01

    Capsule Across the European wintering range Bar-tailed Godwits Limosa lapponica lapponica selected polychaete worms and especially Ragworms Hediste diversicolor, with differences between areas due to variations in prey availability.Aims To determine the diet of Bar-tailed Godwits across their

  14. Spliced RNA of woodchuck hepatitis virus.

    Science.gov (United States)

    Ogston, C W; Razman, D G

    1992-07-01

    Polymerase chain reaction was used to investigate RNA splicing in liver of woodchucks infected with woodchuck hepatitis virus (WHV). Two spliced species were detected, and the splice junctions were sequenced. The larger spliced RNA has an intron of 1300 nucleotides, and the smaller spliced sequence shows an additional downstream intron of 1104 nucleotides. We did not detect singly spliced sequences from which the smaller intron alone was removed. Control experiments showed that spliced sequences are present in both RNA and DNA in infected liver, showing that the viral reverse transcriptase can use spliced RNA as template. Spliced sequences were detected also in virion DNA prepared from serum. The upstream intron produces a reading frame that fuses the core to the polymerase polypeptide, while the downstream intron causes an inframe deletion in the polymerase open reading frame. Whereas the splicing patterns in WHV are superficially similar to those reported recently in hepatitis B virus, we detected no obvious homology in the coding capacity of spliced RNAs from these two viruses.

  15. Four bars inn; Four bars inn

    Energy Technology Data Exchange (ETDEWEB)

    Nishiumi, T. [National Defense Academy, Kanagawa (Japan)

    1999-05-15

    The name Four Bars Inn puns on four drinking bars and four bars on a musical score. It is a public house sited on the busy St. Mary Street, Cardiff, England. During my stay in that town, I often attended the regular jam session that opened at the bar at nine o`clock every Monday evening. A jam session is an event in which any amateur player, and a professional artist occasionally, is allowed to come on the stage freely and to play jazz, the participation fee as low as 300-yen. It is an occasion that provides a friendly meeting of man and woman, young and old, everyone carrying a pint of ale. Senior people happily talking to young ones aged like their grandchildren certainly presents a heart-warming scene, which we scarcely encounter in Japan. The affection that the British entertain toward their domestic furnishings relayed down through many a generation may lead to their respect for senior citizens. I heartily look forward detecting like scenes some day at drinking spots in Japan where the consumption-happy days are over. (NEDO)

  16. Aberrant alternative splicing is another hallmark of cancer.

    Science.gov (United States)

    Ladomery, Michael

    2013-01-01

    The vast majority of human genes are alternatively spliced. Not surprisingly, aberrant alternative splicing is increasingly linked to cancer. Splice isoforms often encode proteins that have distinct and even antagonistic properties. The abnormal expression of splice factors and splice factor kinases in cancer changes the alternative splicing of critically important pre-mRNAs. Aberrant alternative splicing should be added to the growing list of cancer hallmarks.

  17. Aberrant Alternative Splicing Is Another Hallmark of Cancer

    OpenAIRE

    Ladomery, Michael

    2013-01-01

    The vast majority of human genes are alternatively spliced. Not surprisingly, aberrant alternative splicing is increasingly linked to cancer. Splice isoforms often encode proteins that have distinct and even antagonistic properties. The abnormal expression of splice factors and splice factor kinases in cancer changes the alternative splicing of critically important pre-mRNAs. Aberrant alternative splicing should be added to the growing list of cancer hallmarks.

  18. Exotic open-flavor $bc\\bar{q}\\bar{q}$, $bc\\bar{s}\\bar{s}$ and $qc\\bar{q}\\bar{b}$, $sc\\bar{s}\\bar{b}$ tetraquark states

    OpenAIRE

    Chen, Wei; Steele, T. G.; Zhu, Shi-Lin

    2013-01-01

    We study the exotic $bc\\bar{q}\\bar{q}$, $bc\\bar{s}\\bar{s}$ and $qc\\bar{q}\\bar{b}$, $sc\\bar{s}\\bar{b}$ systems by constructing the corresponding tetraquark currents with $J^P=0^+$ and $1^+$. After investigating the two-point correlation functions and the spectral densities, we perform QCD sum rule analysis and extract the masses of these open-flavor tetraquark states. Our results indicate that the masses of both the scalar and axial vector tetraquark states are about $7.1-7.2$ GeV for the $bc\\...

  19. Bus Route Design with a Bayesian Network Analysis of Bus Service Revenues

    Directory of Open Access Journals (Sweden)

    Yi Liu

    2018-01-01

    Full Text Available A Bayesian network is used to estimate revenues of bus services in consideration of the effect of bus travel demands, passenger transport distances, and so on. In this research, the area X in Beijing has been selected as the study area because of its relatively high bus travel demand and, on the contrary, unsatisfactory bus services. It is suggested that the proposed Bayesian network approach is able to rationally predict the probabilities of different revenues of various route services, from the perspectives of both satisfying passenger demand and decreasing bus operation cost. This way, the existing bus routes in the studied area can be optimized for their most probable high revenues.

  20. Bus and Tag Terminators for IBM system/360

    CERN Multimedia

    Control units were connected to the channels with "Bus and Tag" cable pairs. The bus cables carried the address and data information and the tag cables identified what data was on the bus. There were three general types of bus-and-tag cables produced by IBM.

  1. PCISIM - A Simulation Tool for PCI Bus Based Systems

    DEFF Research Database (Denmark)

    Sharp, Robin

    1999-01-01

    This document describes a PCI bus simulator for use in evaluating the feasibility of system designs based on this bus.......This document describes a PCI bus simulator for use in evaluating the feasibility of system designs based on this bus....

  2. SplicePlot: a utility for visualizing splicing quantitative trait loci.

    Science.gov (United States)

    Wu, Eric; Nance, Tracy; Montgomery, Stephen B

    2014-04-01

    RNA sequencing has provided unprecedented resolution of alternative splicing and splicing quantitative trait loci (sQTL). However, there are few tools available for visualizing the genotype-dependent effects of splicing at a population level. SplicePlot is a simple command line utility that produces intuitive visualization of sQTLs and their effects. SplicePlot takes mapped RNA sequencing reads in BAM format and genotype data in VCF format as input and outputs publication-quality Sashimi plots, hive plots and structure plots, enabling better investigation and understanding of the role of genetics on alternative splicing and transcript structure. Source code and detailed documentation are available at http://montgomerylab.stanford.edu/spliceplot/index.html under Resources and at Github. SplicePlot is implemented in Python and is supported on Linux and Mac OS. A VirtualBox virtual machine running Ubuntu with SplicePlot already installed is also available.

  3. Experimental determinations of the eigenmodes for composite bars made with carbon and Kevlar-carbon fibers

    Science.gov (United States)

    Miriţoiu, C. M.; Stănescu, M. M.; Burada, C. O.; Bolcu, D.; Roşca, V.

    2015-11-01

    For modal identification, the single-point excitation method has been widely used in modal tests and it consists in applying a force in a given point and recording the vibratory structure response in all interest points, including the excitation point. There will be presented the experimental recordings for the studied bars (with Kevlar-carbon or carbon fibers), the frequency response function in Cartesian and polar coordinates. By using the frequency response functions we determine the eigenparameters for each bar. We present the final panel of the eigenmodes (with the damping factors, eigenfrequencies and critical damping) for each considered bar. Using the eigenfrequency of the first determined eigenmode, the bars stiffness has been determined. The presented bars can be used in practical engineering for: car or bus body parts, planes body parts, bullet-proof vests, reinforcements for sandwich beams, and so on.

  4. Capacity of columns with splice imperfections

    International Nuclear Information System (INIS)

    Popov, E.P.; Stephen, R.M.

    1977-01-01

    To study the behavior of spliced columns subjected to tensile forces simulating situations which may develop in an earthquake, all of the spliced specimens were tested to failure in tension after first having been subjected to large compressive loads. The results of these tests indicate that the lack of perfect contact at compression splices of columns may not be important, provided that the gaps are shimmed and welding is used to maintain the sections in alignment

  5. The connection between splicing and cancer

    OpenAIRE

    Srebrow, Anabella; Kornblihtt, Alberto Rodolfo

    2017-01-01

    Alternative splicing is a crucial mechanism for generating protein diversity. Different splice variants of a given protein can display different and even antagonistic biological functions. Therefore, appropriate control of their synthesis is required to assure the complex orchestration of cellular processes within multicellular organisms. Mutations in cisacting splicing elements or changes in the activity of regulatory proteins that compromise the accuracy of either constitutive or alternativ...

  6. Large truck and bus crash facts, 2010.

    Science.gov (United States)

    2012-09-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and : property damage only crashes involving large trucks and buses in 2010. Selected crash statistics on passenger : vehicles are also presen...

  7. Large truck and bus crash facts, 2012.

    Science.gov (United States)

    2014-06-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and property damage only crashes involving large trucks and buses in 2012. Selected crash statistics on passenger vehicles are also presented ...

  8. Large truck and bus crash facts, 2013.

    Science.gov (United States)

    2015-04-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and property damage only crashes involving large trucks and buses in 2013. Selected crash statistics on passenger vehicles are also presented ...

  9. Large truck and bus crash facts, 2009.

    Science.gov (United States)

    2011-10-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and : property damage only crashes involving large trucks and buses in 2009. Selected crash statistics on passenger : vehicles are also presen...

  10. Large truck and bus crash facts, 2011.

    Science.gov (United States)

    2013-10-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and : property damage only crashes involving large trucks and buses in 2011. Selected crash statistics on passenger : vehicles are also presen...

  11. Passenger bus industry weather information application.

    Science.gov (United States)

    2011-03-21

    Adverse weather significantly affects the United States national transportation system, including commercial companies : that rely on highways to support their enterprises. The Passenger Bus (Motorcoach) Industry (PBI) is one such affected : user who...

  12. Understanding & modeling bus transit driver availability.

    Science.gov (United States)

    2014-07-01

    Bus transit agencies are required to hire extraboard (i.e. back-up) operators to account for unexpected absences. Incorrect sizing of extra driver workforce is problematic for a number of reasons. Overestimating the appropriate number of extraboard o...

  13. Bicycle-bus conflict area study.

    Science.gov (United States)

    2009-11-01

    Increasing bicycle use and bus ridership are both desirable policy goals : from a sustainability standpoint, but on city streets these two modes of : transport are often in conflict. While occupying opposite ends of the size : and weight spectrum, th...

  14. Alternative Splicing in Neurogenesis and Brain Development.

    Science.gov (United States)

    Su, Chun-Hao; D, Dhananjaya; Tarn, Woan-Yuh

    2018-01-01

    Alternative splicing of precursor mRNA is an important mechanism that increases transcriptomic and proteomic diversity and also post-transcriptionally regulates mRNA levels. Alternative splicing occurs at high frequency in brain tissues and contributes to every step of nervous system development, including cell-fate decisions, neuronal migration, axon guidance, and synaptogenesis. Genetic manipulation and RNA sequencing have provided insights into the molecular mechanisms underlying the effects of alternative splicing in stem cell self-renewal and neuronal fate specification. Timely expression and perhaps post-translational modification of neuron-specific splicing regulators play important roles in neuronal development. Alternative splicing of many key transcription regulators or epigenetic factors reprograms the transcriptome and hence contributes to stem cell fate determination. During neuronal differentiation, alternative splicing also modulates signaling activity, centriolar dynamics, and metabolic pathways. Moreover, alternative splicing impacts cortical lamination and neuronal development and function. In this review, we focus on recent progress toward understanding the contributions of alternative splicing to neurogenesis and brain development, which has shed light on how splicing defects may cause brain disorders and diseases.

  15. Alternative Splicing in Neurogenesis and Brain Development

    Directory of Open Access Journals (Sweden)

    Chun-Hao Su

    2018-02-01

    Full Text Available Alternative splicing of precursor mRNA is an important mechanism that increases transcriptomic and proteomic diversity and also post-transcriptionally regulates mRNA levels. Alternative splicing occurs at high frequency in brain tissues and contributes to every step of nervous system development, including cell-fate decisions, neuronal migration, axon guidance, and synaptogenesis. Genetic manipulation and RNA sequencing have provided insights into the molecular mechanisms underlying the effects of alternative splicing in stem cell self-renewal and neuronal fate specification. Timely expression and perhaps post-translational modification of neuron-specific splicing regulators play important roles in neuronal development. Alternative splicing of many key transcription regulators or epigenetic factors reprograms the transcriptome and hence contributes to stem cell fate determination. During neuronal differentiation, alternative splicing also modulates signaling activity, centriolar dynamics, and metabolic pathways. Moreover, alternative splicing impacts cortical lamination and neuronal development and function. In this review, we focus on recent progress toward understanding the contributions of alternative splicing to neurogenesis and brain development, which has shed light on how splicing defects may cause brain disorders and diseases.

  16. TMS communications software. Volume 2: Bus interface unit

    Science.gov (United States)

    Gregor, P. J.

    1979-01-01

    A data bus communication system to support the space shuttle's Trend Monitoring System (TMS) and to provide a basis for evaluation of the bus concept is described. Installation of the system included developing both hardware and software interfaces between the bus and the specific TMS computers and terminals. The software written for the microprocessor-based bus interface units is described. The software implements both the general bus communications protocol and also the specific interface protocols for the TMS computers and terminals.

  17. Comparison and status of 32 bit backplane bus architectures

    International Nuclear Information System (INIS)

    Muller, K.D.

    1985-01-01

    With the introduction of 32 bit microprocessors several new 32 bit backplane bus architectures have been developed and are in the process for standardization. Among these are Future Bus (IEEE P896.1), VME-Bus (IEEE 1014), MULTIBUS II, Nu-Bus and Fastbus (IEEE 960). The paper describes and compares the main features of these bus architectures and mentions the status of national and international standardization efforts

  18. The seated bus passenger--a review.

    Science.gov (United States)

    Levis, J A

    1978-09-01

    The paper describes the inter-relationship of anthropometry, rig studies and dynamic testing of aspects related to problems of the seated bus passenger. It seeks to draw together sub-sections of a very large study sponsored by the government through the Transport and Road Research Laboratory and undertaken by the Human Factors Group of Leyland Truck and Bus. It is relevant to all those designing passenger carrying transport systems.

  19. The Gozo discovery bus : a successful experiment

    OpenAIRE

    Vella, Maryrose

    2008-01-01

    The introduction of a tourist discovery bus in Gozo came about as a result of an EU Project which is part of the Interreg III B Archimed programmes in which the Islands and Small States Institute of the University of Malta participated. Other countries participating in this programme besides Malta, represented by the Islands and Small States Institute, are Italy, Cyprus and Greece. The discovery bus service was aimed at encouraging more tourists to come to Gozo and enabling them to visit stra...

  20. NOC AND BUS ARCHITECTURE: A COMPARISON

    OpenAIRE

    RAJEEV KAMAL; NEERAJ YADAV

    2012-01-01

    Network-on-chip designs promise to offer considerable advantages over the traditional bus-based architecture. As continuing scaling of Moore’s law enables ever greater transistor densities, design complexity, power limitations and application convergence networks have started to replace busses in much smaller systems and the enhancement of NoC. This paper summarizes the advantages of the NoC and the limitations of traditional bus based architecture. In this paper we discuss a detailed compari...

  1. Bus line classification using neural networks

    OpenAIRE

    Jiménez Alonso, Felipe; Serradilla García, Francisco; Román de Andrés, Alfonso; Naranjo Hernandez, Jose Eugenio

    2014-01-01

    Grouping urban bus routes is necessary when there are evidences of significant differences among them. In Jiménez et al. (2013), a reduced sample of routes was grouped into clusters utilizing kinematic measured data. As a further step, in this paper, the remaining urban bus routes of a city, for which no kinematic measurements are available, are classified. For such purpose we use macroscopic geographical and functional variables to describe each route, while the clustering process is perform...

  2. Common bus multinode sensor system

    International Nuclear Information System (INIS)

    Kelly, T.F.; Naviasky, E.H.; Evans, W.P.; Jefferies, D.W.; Smith, J.R.

    1988-01-01

    This patent describes a nuclear power plant including a common bus multinode sensor system for sensors in the nuclear power plant, each sensor producing a sensor signal. The system consists of: a power supply providing power; a communication cable coupled to the power supply; plural remote sensor units coupled between the cable and one or more sensors, and comprising: a direct current power supply, connected to the cable and converting the power on the cable into direct current; an analog-to-digital converter connected to the direct current power supply; an oscillator reference; a filter; and an integrated circuit sensor interface connected to the direct current power supply, the analog-to-digital converter, the oscillator crystal and the filter, the interface comprising: a counter receiving a frequency designation word from external to the interface; a phase-frequency comparator connected to the counter; an oscillator connected to the oscillator reference; a timing counter connected to the oscillator, the phase/frequency comparator and the analog-to-digital converter; an analog multiplexer connectable to the sensors and the analog-to-digital converter, and connected to the timing counter; a shift register operatively connected to the timing counter and the analog-to-digital converter; an encoder connected to the shift register and connectable to the filter; and a voltage controlled oscillator connected to the filter and the cable

  3. SplicingTypesAnno: annotating and quantifying alternative splicing events for RNA-Seq data.

    Science.gov (United States)

    Sun, Xiaoyong; Zuo, Fenghua; Ru, Yuanbin; Guo, Jiqiang; Yan, Xiaoyan; Sablok, Gaurav

    2015-04-01

    Alternative splicing plays a key role in the regulation of the central dogma. Four major types of alternative splicing have been classified as intron retention, exon skipping, alternative 5 splice sites or alternative donor sites, and alternative 3 splice sites or alternative acceptor sites. A few algorithms have been developed to detect splice junctions from RNA-Seq reads. However, there are few tools targeting at the major alternative splicing types at the exon/intron level. This type of analysis may reveal subtle, yet important events of alternative splicing, and thus help gain deeper understanding of the mechanism of alternative splicing. This paper describes a user-friendly R package, extracting, annotating and analyzing alternative splicing types for sequence alignment files from RNA-Seq. SplicingTypesAnno can: (1) provide annotation for major alternative splicing at exon/intron level. By comparing the annotation from GTF/GFF file, it identifies the novel alternative splicing sites; (2) offer a convenient two-level analysis: genome-scale annotation for users with high performance computing environment, and gene-scale annotation for users with personal computers; (3) generate a user-friendly web report and additional BED files for IGV visualization. SplicingTypesAnno is a user-friendly R package for extracting, annotating and analyzing alternative splicing types at exon/intron level for sequence alignment files from RNA-Seq. It is publically available at https://sourceforge.net/projects/splicingtypes/files/ or http://genome.sdau.edu.cn/research/software/SplicingTypesAnno.html. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  4. Security awareness for public bus transportation : case studies of attacks against the Israeli public bus system.

    Science.gov (United States)

    2012-03-01

    This report presents 16 case studies of attacks planned or carried out against Israeli bus targets, along with statistical data on the number, frequency, and lethality of attacks against bus targets that have taken place in Israel since 1970 and duri...

  5. 77 FR 6178 - FY 2012 Discretionary Funding Opportunities: Bus and Bus Facilities Programs (State of Good...

    Science.gov (United States)

    2012-02-07

    ... not limited to: Rail, ferry, intercity bus and private transportation providers. In order to be..., intercity bus, and private transportation providers. In order to be eligible for funding, intermodal... automobile travel, improving the pedestrian environment of a community or, use of environmental design...

  6. Digital processing data communication systems (bus systems). Digitale Prozessdaten-Kommunikations-Systeme (Bus Systeme)

    Energy Technology Data Exchange (ETDEWEB)

    Fleck, K

    1980-01-01

    After an introduction to the technology of digital processing data communication systems there are the following chapters: digital communication of processing data in automation technology, the technology of biserial communication, the implementaiton of a bus system, the data transmission of the TDC-2000 system of Honeywell's and the process bus CS 275 in the automation system TELEPERM M of Siemens AG.

  7. Dynamic Bus Travel Time Prediction Models on Road with Multiple Bus Routes

    Science.gov (United States)

    Bai, Cong; Peng, Zhong-Ren; Lu, Qing-Chang; Sun, Jian

    2015-01-01

    Accurate and real-time travel time information for buses can help passengers better plan their trips and minimize waiting times. A dynamic travel time prediction model for buses addressing the cases on road with multiple bus routes is proposed in this paper, based on support vector machines (SVMs) and Kalman filtering-based algorithm. In the proposed model, the well-trained SVM model predicts the baseline bus travel times from the historical bus trip data; the Kalman filtering-based dynamic algorithm can adjust bus travel times with the latest bus operation information and the estimated baseline travel times. The performance of the proposed dynamic model is validated with the real-world data on road with multiple bus routes in Shenzhen, China. The results show that the proposed dynamic model is feasible and applicable for bus travel time prediction and has the best prediction performance among all the five models proposed in the study in terms of prediction accuracy on road with multiple bus routes. PMID:26294903

  8. Dynamic Bus Travel Time Prediction Models on Road with Multiple Bus Routes.

    Science.gov (United States)

    Bai, Cong; Peng, Zhong-Ren; Lu, Qing-Chang; Sun, Jian

    2015-01-01

    Accurate and real-time travel time information for buses can help passengers better plan their trips and minimize waiting times. A dynamic travel time prediction model for buses addressing the cases on road with multiple bus routes is proposed in this paper, based on support vector machines (SVMs) and Kalman filtering-based algorithm. In the proposed model, the well-trained SVM model predicts the baseline bus travel times from the historical bus trip data; the Kalman filtering-based dynamic algorithm can adjust bus travel times with the latest bus operation information and the estimated baseline travel times. The performance of the proposed dynamic model is validated with the real-world data on road with multiple bus routes in Shenzhen, China. The results show that the proposed dynamic model is feasible and applicable for bus travel time prediction and has the best prediction performance among all the five models proposed in the study in terms of prediction accuracy on road with multiple bus routes.

  9. Alternative Splicing Control of Abiotic Stress Responses.

    Science.gov (United States)

    Laloum, Tom; Martín, Guiomar; Duque, Paula

    2018-02-01

    Alternative splicing, which generates multiple transcripts from the same gene, is an important modulator of gene expression that can increase proteome diversity and regulate mRNA levels. In plants, this post-transcriptional mechanism is markedly induced in response to environmental stress, and recent studies have identified alternative splicing events that allow rapid adjustment of the abundance and function of key stress-response components. In agreement, plant mutants defective in splicing factors are severely impaired in their response to abiotic stress. Notably, mounting evidence indicates that alternative splicing regulates stress responses largely by targeting the abscisic acid (ABA) pathway. We review here current understanding of post-transcriptional control of plant stress tolerance via alternative splicing and discuss research challenges for the near future. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Classifying the Diversity of Bus Mapping Systems

    Science.gov (United States)

    Said, Mohd Shahmy Mohd; Forrest, David

    2018-05-01

    This study represents the first stage of an investigation into understanding the nature of different approaches to mapping bus routes and bus network, and how they may best be applied in different public transport situations. In many cities, bus services represent an important facet of easing traffic congestion and reducing pollution. However, with the entrenched car culture in many countries, persuading people to change their mode of transport is a major challenge. To promote this modal shift, people need to know what services are available and where (and when) they go. Bus service maps provide an invaluable element of providing suitable public transport information, but are often overlooked by transport planners, and are under-researched by cartographers. The method here consists of the creation of a map evaluation form and performing assessment of published bus networks maps. The analyses were completed by a combination of quantitative and qualitative data analysis of various aspects of cartographic design and classification. This paper focuses on the resulting classification, which is illustrated by a series of examples. This classification will facilitate more in depth investigations into the details of cartographic design for such maps and help direct areas for user evaluation.

  11. Impartial Triangular Chocolate Bar Games

    OpenAIRE

    Miyadera, Ryohei; Nakamura, Shunsuke; Fukui, Masanori

    2017-01-01

    Chocolate bar games are variants of the game of Nim in which the goal is to leave your opponent with the single bitter part of the chocolate bar. The rectangular chocolate bar game is a thinly disguised form of classical multi-heap Nim. In this work, we investigate the mathematical structure of triangular chocolate bar games in which the triangular chocolate bar can be cut in three directions. In the triangular chocolate bar game, a position is a $\\mathcal{P}$-position if and only if $x \\oplu...

  12. Breaking through the Bar

    Science.gov (United States)

    Gray, Katti

    2011-01-01

    Howard University School of Law had a problem, and school officials knew it. Over a 20-year period, 40 percent of its graduates who took the Maryland bar exam failed it on their first try. During the next 24 months--the time frame required to determine its "eventual pass rate"--almost 90 percent of the students did pass. What they did…

  13. Raising the bar (7)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, Maria; Amaral, Pedro; Bhattacharjee, Arnab; Corrado, Luisa; Doran, Justin; Fingleton, Bernard; Fuerst, Franz; Garretsen, Harry; Igliori, Danilo; Gallo, Julie Le; McCann, Philip; Monastiriotis, Vassilis; Quatraro, Francesco; Yu, Jihai

    2018-01-01

    This editorial summarises the papers published in issue 13.1 so as to raise the bar in applied spatial economic research and highlight new trends. The first paper adopts a scale neutral approach to investigate the spatial mechanisms that cause regional innovation and growth. The second paper claims

  14. Raising the Bar (3)

    NARCIS (Netherlands)

    Elhorst, Paul; Abreu, M.; Amaral, P.; Bhattacharjee, A.; Corrado, L.; Fingleton, B.; Fuerst, F.; Garretsen, H.; Igliori, D.; Le Gallo, J.; McCann, P.; Monastiriotis, V.; Pryce, G.; Yu, J.

    This editorial summarizes and comments on the papers published in issue 11(3) so as to raise the bar in applied spatial economic research and highlight new trends. The first paper proposes spatial and a-spatial indicators to describe the networks of airline companies around the world. The second

  15. Spliced

    DEFF Research Database (Denmark)

    Addison, Courtney Page

    2017-01-01

    Human gene therapy (HGT) aims to cure disease by inserting or editing the DNA of patients with genetic conditions. Since foundational genetic techniques came into use in the 1970s, the field has developed to the point that now three therapies have market approval, and over 1800 clinical trials have...

  16. Position dependence of the rous sarcoma virus negative regulator of splicing element reflects proximity to a 5' splice site

    International Nuclear Information System (INIS)

    Wang Yuedi; McNally, Mark T.

    2003-01-01

    Rous sarcoma virus (RSV) requires incomplete splicing of its viral transcripts to maintain efficient replication. A splicing inhibitor element, the negative regulator of splicing (NRS), is located near the 5' end of the RNA but the significance of this positioning is not known. In a heterologous intron the NRS functions optimally when positioned close to the authentic 5' splice site. This observation led us to investigate the basis of the position dependence. Four explanations were put forth and stressed the role of three major elements involved in splicing, the 3' splice site, the 5' splice site, and the 5' end cap structure. NRS function was unrelated to its position relative to the 3' splice site or the cap structure and appeared to depend on its position relative to the authentic 5' splice site. We conclude that position dependence may reflect distance constraints necessary for competition of the NRS with the authentic 5' splice site for pairing with the 3' splice sites

  17. VICbus: A standard inter-crate bus

    International Nuclear Information System (INIS)

    Parkman, C.F.

    1990-01-01

    VICbus is a standard inter-crate cable bus being developed by a working group of the ISO/IEC. Derived from an initiative of the VMEbus Working Group of ESONE, VICbus aims to provide users of multi-crate VMEbus and other backplane bus systems with a standard inter-crate connection. Multi-drop operation is provided for up to 31 devices on a cable of maximum 100 metres in length. Two data transfer protocols are specified, compelled (asynchronous) transfers for transparent interconnection of backplane bus systems (VMEbus in the first instance), and high-speed, non-compelled (synchronous) transfers between VICbus interfaces. A limited interrupt mechanism is specified, as well as simple, but efficient arbitration technique. Conventional technology is employed: copper cables, existing transcievers, and differential signal transmission, together with proven low-level protocols. Data transfer rates in excess of 30 Mbyte/s will be possible on a cable length of 30 metres

  18. Depolarization-mediated regulation of alternative splicing

    Directory of Open Access Journals (Sweden)

    Alok eSharma

    2011-12-01

    Full Text Available Alternative splicing in eukaryotes plays an important role in regulating gene expression by selectively including alternative exons. A wealth of information has been accumulated that explains how alternative exons are selected in a developmental stage- or tissue-specific fashion. However, our knowledge of how cells respond to environmental changes to alter alternative splicing is very limited. For example, although a number of alternative exons have been shown to be regulated by calcium level alterations, the underlying mechanisms are not well understood. As calcium signaling in neurons plays a crucial role in essential neuronal functions such as learning and memory formation, it is important to understand how this process is regulated at every level in gene expression. The significance of the dynamic control of alternative splicing in response to changes of calcium levels has been largely unappreciated. In this communication, we will summarize the recent advances in calcium signaling-mediated alternative splicing that have provided some insights into the important regulatory mechanisms. In addition to describing the cis-acting RNA elements on the pre-mRNA molecules that respond to changes of intracellular calcium levels, we will summarize how splicing regulators change and affect alternative splicing in this process. We will also discuss a novel mode of calcium-mediated splicing regulation at the level of chromatin structure and transcription.

  19. Risk factors affecting fatal bus accident severity: Their impact on different types of bus drivers.

    Science.gov (United States)

    Feng, Shumin; Li, Zhenning; Ci, Yusheng; Zhang, Guohui

    2016-01-01

    While the bus is generally considered to be a relatively safe means of transportation, the property losses and casualties caused by bus accidents, especially fatal ones, are far from negligible. The reasons for a driver to incur fatalities are different in each case, and it is essential to discover the underlying risk factors of bus fatality severity for different types of drivers in order to improve bus safety. The current study investigates the underlying risk factors of fatal bus accident severity to different types of drivers in the U.S. by estimating an ordered logistic model. Data for the analysis are retrieved from the Buses Involved in Fatal Accidents (BIFA) database from the USA for the years 2006-2010. Accidents are divided into three levels by counting their equivalent fatalities, and the drivers are classified into three clusters by the K-means cluster analysis. The analysis shows that some risk factors have the same impact on different types of drivers, they are: (a) season; (b) day of week; (c) time period; (d) number of vehicles involved; (e) land use; (f) manner of collision; (g) speed limit; (h) snow or ice surface condition; (i) school bus; (j) bus type and seating capacity; (k) driver's age; (l) driver's gender; (m) risky behaviors; and (n) restraint system. Results also show that some risk factors only have impact on the "young and elder drivers with history of traffic violations", they are: (a) section type; (b) number of lanes per direction; (c) roadway profile; (d) wet road surface; and (e) cyclist-bus accident. Notably, history of traffic violations has different impact on different types of bus drivers. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Bar codes for nuclear safeguards

    International Nuclear Information System (INIS)

    Keswani, A.N.; Bieber, A.M. Jr.

    1983-01-01

    Bar codes similar to those used in supermarkets can be used to reduce the effort and cost of collecting nuclear materials accountability data. A wide range of equipment is now commercially available for printing and reading bar-coded information. Several examples of each of the major types of commercially available equipment are given, and considerations are discussed both for planning systems using bar codes and for choosing suitable bar code equipment

  1. Bar codes for nuclear safeguards

    International Nuclear Information System (INIS)

    Keswani, A.N.; Bieber, A.M.

    1983-01-01

    Bar codes similar to those used in supermarkets can be used to reduce the effort and cost of collecting nuclear materials accountability data. A wide range of equipment is now commercially available for printing and reading bar-coded information. Several examples of each of the major types of commercially-available equipment are given, and considerations are discussed both for planning systems using bar codes and for choosing suitable bar code equipment

  2. RAGE splicing variants in mammals.

    Science.gov (United States)

    Sterenczak, Katharina Anna; Nolte, Ingo; Murua Escobar, Hugo

    2013-01-01

    The receptor for advanced glycation end products (RAGE) is a multiligand receptor of environmental stressors which plays key roles in pathophysiological processes, including immune/inflammatory disorders, Alzheimer's disease, diabetic arteriosclerosis, tumorigenesis, and metastasis. Besides the full-length RAGE protein in humans nearly 20 natural occurring RAGE splicing variants were described on mRNA and protein level. These naturally occurring isoforms are characterized by either N-terminally or C-terminally truncations and are discussed as possible regulators of the full-length RAGE receptor either by competitive ligand binding or by displacing the full-length protein in the membrane. Accordingly, expression deregulations of the naturally occurring isoforms were supposed to have significant effect on RAGE-mediated disorders. Thereby the soluble C-truncated RAGE isoforms present in plasma and tissues are the mostly focused isoforms in research and clinics. Deregulations of the circulating levels of soluble RAGE forms were reported in several RAGE-associated pathological disorders including for example atherosclerosis, diabetes, renal failure, Alzheimer's disease, and several cancer types. Regarding other mammalian species, the canine RAGE gene showed high similarities to the corresponding human structures indicating RAGE to be evolutionary highly conserved between both species. Similar to humans the canine RAGE showed a complex and extensive splicing activity leading to a manifold pattern of RAGE isoforms. Due to the similarities seen in several canine and human diseases-including cancer-comparative structural and functional analyses allow the development of RAGE and ligand-specific therapeutic approaches beneficial for human and veterinary medicine.

  3. The Bus Station Spacing Optimization Based on Game Theory

    Directory of Open Access Journals (Sweden)

    Changjiang Zheng

    2015-01-01

    Full Text Available With the development of city, the problem of traffic is becoming more and more serious. Developing public transportation has become the key to solving this problem in all countries. Based on the existing public transit network, how to improve the bus operation efficiency, and reduce the residents transit trip cost has become a simple and effective way to develop the public transportation. Bus stop spacing is an important factor affecting passengers’ travel time. How to set up bus stop spacing has become the key to reducing passengers’ travel time. According to comprehensive traffic survey, theoretical analysis, and summary of urban public transport characteristics, this paper analyzes the impact of bus stop spacing on passenger in-bus time cost and out-bus time cost and establishes in-bus time and out-bus time model. Finally, the paper gets the balance best station spacing by introducing the game theory.

  4. Bus accident severity and passenger injury: evidence from Denmark

    DEFF Research Database (Denmark)

    Prato, Carlo Giacomo; Kaplan, Sigal

    2014-01-01

    Purpose Bus safety is a concern not only in developing countries, but also in the U.S. and Europe. In Denmark, disentangling risk factors that are positively or negatively related to bus accident severity and injury occurrence to bus passengers can contribute to promote safety as an essential...... principle of sustainable transit and advance the vision “every accident is one too many”. Methods Bus accident data were retrieved from the national accident database for the period 2002–2011. A generalized ordered logit model allows analyzing bus accident severity and a logistic regression enables...... examining occurrence of injury to bus passengers. Results Bus accident severity is positively related to (i) the involvement of vulnerable road users, (ii) high speed limits, (iii) night hours, (iv) elderly drivers of the third party involved, and (v) bus drivers and other drivers crossing in yellow or red...

  5. Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus Preliminary Evaluation Results

    Science.gov (United States)

    2008-10-16

    This report describes operations at Connecticut Transit (CTTRANSIT) in Hartford for one prototype fuel cell bus and three new diesel buses operating from the same location. The report discusses the planned fuel cell bus demonstration and equipment us...

  6. Request-Centric Wireless Bus Information Management System

    Directory of Open Access Journals (Sweden)

    Ying-Chih Chen

    2016-11-01

    Full Text Available This invention relates to a wireless bus information management system, which includes bus stop and vehicle management subsystems. The management signals are transmittable between bus stops and the vehicle. Based on vehicle management signals, the bus stop management subsystem can obtain information about the bus route identification, the number of unoccupied seats, the intention to stop or not, etc. Similarly, with the bus stop management signals, the vehicle management subsystem can make the decision of stopping. Accordingly, when a passenger wants to get off the vehicle or there are unoccupied seats, the vehicle management subsystem will inform the bus stop management subsystem such that the passengers waiting at the bus stop may flexibly schedule their travel plan. The proposed distributed wireless system is detailed by a prototype implementation and a simulation analysis, which is shown to be feasible and scalable.

  7. Splicing pattern - ASTRA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us ASTRA Splicing pattern Data detail Data name Splicing pattern DOI 10.18908/lsdba.nbdc00371-0...04 Description of data contents The patterns of alternative splicing/transcriptional initiation Data file Fi...le name: astra_splicing_pattern.zip File URL: ftp://ftp.biosciencedbc.jp/archive/astra/LATEST/astra_splicing_patt...ogodb/view/astra_splicing_pattern#en Data acquisition method For the five organisms (H. sapiens, M. musculus...apping data into bit arrays, detection of splicing patterns and distribution to t

  8. Cooling of rectangular bars

    International Nuclear Information System (INIS)

    Frainer, V.J.

    1979-01-01

    A solution of the time-transient Heat Transfer Differential Equation in rectangular coordinates is presented, leading to a model which describes the temperature drop with time in rectangular bars. It is similar to an other model for cilindrical bars which has been previously developed in the Laboratory of Mechanical Metallurgy of UFRGS. Following these models, a generalization has been made, which permits cooling time evaluation for all profiles. These results are compared with experimental laboratory data in the 1200 to 800 0 C range. Some other existing models were also studied which have the purpose of studing the same phenomenon. Their mathematical forms and their evaluated values are analyzed and compared with experimental ones. (Author) [pt

  9. Analysis of a diesel-electric hybrid urban bus system

    Science.gov (United States)

    Marr, W. W.; Sekar, R. R.; Ahlheim, M. C.

    A hybrid bus powered by a diesel engine and a battery pack was analyzed over an idealized bus-driving cycle in Chicago. Three hybrid configurations, two parallel and one series, were evaluated. The results indicate that the fuel economy of a hybrid bus, taking into account the regenerative braking, is comparable with that of a conventional diesel bus. Life-cycle costs are slightly higher because of the added weight and cost of the battery.

  10. The Possible Heavy Tetraquarks $qQ\\bar q \\bar Q$, $qq\\bar Q \\bar Q$ and $qQ\\bar Q \\bar Q$

    OpenAIRE

    Cui, Ying; Chen, Xiao-Lin; Deng, Wei-Zhen; Zhu, Shi-Lin

    2006-01-01

    Assuming X(3872) is a $qc \\bar q \\bar c$ tetraquark and using its mass as input, we perform a schematic study of the masses of possible heavy tetraquarks using the color-magnetic interaction with the flavor symmetry breaking corrections.

  11. Human Splicing Finder: an online bioinformatics tool to predict splicing signals.

    Science.gov (United States)

    Desmet, François-Olivier; Hamroun, Dalil; Lalande, Marine; Collod-Béroud, Gwenaëlle; Claustres, Mireille; Béroud, Christophe

    2009-05-01

    Thousands of mutations are identified yearly. Although many directly affect protein expression, an increasing proportion of mutations is now believed to influence mRNA splicing. They mostly affect existing splice sites, but synonymous, non-synonymous or nonsense mutations can also create or disrupt splice sites or auxiliary cis-splicing sequences. To facilitate the analysis of the different mutations, we designed Human Splicing Finder (HSF), a tool to predict the effects of mutations on splicing signals or to identify splicing motifs in any human sequence. It contains all available matrices for auxiliary sequence prediction as well as new ones for binding sites of the 9G8 and Tra2-beta Serine-Arginine proteins and the hnRNP A1 ribonucleoprotein. We also developed new Position Weight Matrices to assess the strength of 5' and 3' splice sites and branch points. We evaluated HSF efficiency using a set of 83 intronic and 35 exonic mutations known to result in splicing defects. We showed that the mutation effect was correctly predicted in almost all cases. HSF could thus represent a valuable resource for research, diagnostic and therapeutic (e.g. therapeutic exon skipping) purposes as well as for global studies, such as the GEN2PHEN European Project or the Human Variome Project.

  12. Security awareness for public bus transportation : case studies of attacks against the Israeli public bus system [research brief].

    Science.gov (United States)

    2012-03-01

    Government regulators and transportation operators of public bus systems are concerned about terrorist attacks. Bus attacks in Israel between late September 2000 and the end of 2006, a period known as the Second Intifada, are particularly well known....

  13. Triply heavy tetraquark states with the $QQ\\bar{Q}\\bar{q}$ configuration

    OpenAIRE

    Chen, Kan; Liu, Xiang; Wu, Jing; Liu, Yan-Rui; Zhu, Shi-Lin

    2016-01-01

    In the framework of the color-magnetic interaction, we systematically investigate the mass splittings of the $QQ\\bar{Q}\\bar{q}$ tetraquark states and estimated their rough masses in this work. These systems include the explicitly exotic states $cc\\bar{b}\\bar{q}$ and $bb\\bar{c}\\bar{q}$ and the hidden exotic states $cc\\bar{c}\\bar{q}$, $cb\\bar{b}\\bar{q}$, $bc\\bar{c}\\bar{q}$, and $bb\\bar{b}\\bar{q}$. If a state around the estimated mass region could be observed, its nature as a genuine tetraquark ...

  14. 76 FR 68819 - State of Good Repair Bus and Bus Facilities Discretionary Program Funds

    Science.gov (United States)

    2011-11-07

    ... Availability on June 24, 2011. The SGR Initiative makes funds available to public transit providers to finance capital projects to replace, rehabilitate, and purchase buses and related equipment and to construct bus...

  15. 76 FR 37393 - FY 2011 Discretionary Livability Funding Opportunity; Section 5309 Bus and Bus Facilities...

    Science.gov (United States)

    2011-06-27

    ... 90 percent Federal share for the total cost of a biodiesel bus. The Act also allows a 90 percent... transit systems or roadways. iv. Will improve accessibility and transport services for economically...

  16. HOLLYWOOD: a comparative relational database of alternative splicing.

    Science.gov (United States)

    Holste, Dirk; Huo, George; Tung, Vivian; Burge, Christopher B

    2006-01-01

    RNA splicing is an essential step in gene expression, and is often variable, giving rise to multiple alternatively spliced mRNA and protein isoforms from a single gene locus. The design of effective databases to support experimental and computational investigations of alternative splicing (AS) is a significant challenge. In an effort to integrate accurate exon and splice site annotation with current knowledge about splicing regulatory elements and predicted AS events, and to link information about the splicing of orthologous genes in different species, we have developed the Hollywood system. This database was built upon genomic annotation of splicing patterns of known genes derived from spliced alignment of complementary DNAs (cDNAs) and expressed sequence tags, and links features such as splice site sequence and strength, exonic splicing enhancers and silencers, conserved and non-conserved patterns of splicing, and cDNA library information for inferred alternative exons. Hollywood was implemented as a relational database and currently contains comprehensive information for human and mouse. It is accompanied by a web query tool that allows searches for sets of exons with specific splicing characteristics or splicing regulatory element composition, or gives a graphical or sequence-level summary of splicing patterns for a specific gene. A streamlined graphical representation of gene splicing patterns is provided, and these patterns can alternatively be layered onto existing information in the UCSC Genome Browser. The database is accessible at http://hollywood.mit.edu.

  17. Protein splicing and its evolution in eukaryotes

    Directory of Open Access Journals (Sweden)

    Starokadomskyy P. L.

    2010-02-01

    Full Text Available Inteins, or protein introns, are parts of protein sequences that are post-translationally excised, their flanking regions (exteins being spliced together. This process was called protein splicing. Originally inteins were found in prokaryotic or unicellular eukaryotic organisms. But the general principles of post-translation protein rearrangement are evolving yielding different post-translation modification of proteins in multicellular organisms. For clarity, these non-intein mediated events call either protein rearrangements or protein editing. The most intriguing example of protein editing is proteasome-mediated splicing of antigens in vertebrates that may play important role in antigen presentation. Other examples of protein rearrangements are maturation of Hg-proteins (critical receptors in embryogenesis as well as maturation of several metabolic enzymes. Despite a lack of experimental data we try to analyze some intriguing examples of protein splicing evolution.

  18. Advanced Competencies for School Bus Drivers.

    Science.gov (United States)

    Illinois State Board of Education, Springfield.

    Four units are provided for formal classroom instruction in advanced competencies for school bus drivers in Illinois. Units cover passenger control, accidents and emergencies, detecting hazards, and first aid. Each unit contains some or all of the following components: table of contents; a list of objectives; informative material, including an…

  19. Functional Bus Driver-Pupil Passenger Relationships.

    Science.gov (United States)

    Farmer, Ernest

    1987-01-01

    Successful school bus drivers bring much more than mechanical know-how to the job. They develop good rapport with students while acting to bring undesirable student behavior under control. Drivers must also show an interest in students' welfare and have a good sense of humor. (MLH)

  20. School Bus Accidents: Reducing Incidents and Injuries

    Science.gov (United States)

    Mahoney, Daniel

    2009-01-01

    The number of children injured in nonfatal school bus accidents annually is more than double the number previously estimated. In Ohio alone, approximately 20,800 children younger than 18 were occupants of school buses that were involved in crashes in 2003 and 2004 (McGeehan 2007). Among those children, most had minor or no injuries. However, there…

  1. An Experimental Platform for Autonomous Bus Development

    Directory of Open Access Journals (Sweden)

    Héctor Montes

    2017-11-01

    Full Text Available Nowadays, with highly developed instrumentation, sensing and actuation technologies, it is possible to foresee an important advance in the field of autonomous and/or semi-autonomous transportation systems. Intelligent Transport Systems (ITS have been subjected to very active research for many years, and Bus Rapid Transit (BRT is one area of major interest. Among the most promising transport infrastructures, the articulated bus is an interesting, low cost, high occupancy capacity and friendly option. In this paper, an experimental platform for research on the automatic control of an articulated bus is presented. The aim of the platform is to allow full experimentation in real conditions for testing technological developments and control algorithms. The experimental platform consists of a mobile component (a commercial articulated bus fully instrumented and a ground test area composed of asphalt roads inside the Consejo Superior de Investigaciones Científicas (CSIC premises. This paper focuses also on the development of a human machine interface to ease progress in control system evaluation. Some experimental results are presented in order to show the potential of the proposed platform.

  2. Bus safety study : a report to Congress.

    Science.gov (United States)

    2013-11-01

    Section 20021(b) of the Moving Ahead for Progress for the 21st Century (MAP-21) legislation requires the Secretary of Transportation : to submit a report of the results of a Bus Safety Study to the Committee on Banking, Housing, and Urban Affai...

  3. Technology Validation: Fuel Cell Bus Evaluations

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2018-01-02

    This presentation describing the FY 2016 accomplishments for the National Renewable Energy Laboratory's Fuel Cell Bus Evaluations project was presented at the U.S. Department of Energy Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting, June 7, 2016.

  4. Repeater For A Digital-Communication Bus

    Science.gov (United States)

    Torres-Guzman, Esteban; Olson, Stephen; Heaps, Tim

    1993-01-01

    Digital repeater circuit designed to extend range of communication on MIL-STD-1553 bus beyond original maximum allowable length of 300 ft. Circuit provides two-way communication, one way at time, and conforms to specifications of MIL-STD-1553. Crosstalk and instability eliminated.

  5. Energy DataBus (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2013-07-01

    NREL has developed the Energy DataBus, an open-sourced software that collects massive amounts of energy-related data at second-to-second intervals; stores it in a massive, scalable database; and turns it into useful information.

  6. Stochastic Bus Dispatching Model untuk Optimalisasi Jumlah Bus Transjakarta Koridor 3

    Directory of Open Access Journals (Sweden)

    Zahedi Zahedi

    2011-12-01

    Full Text Available The increasing number of road user causing traffic jam finds out a way, busway. However, in its implementation, the number of fleet available does not enough to take the passengers. It results in the an amassed of passengers at several bus stops. This leads to passenger dissatisfaction satisfaction which eventually causes Transjakarta users to reuse private cars. Definitely this situation may builds up traffic jams. Stochastic Bus Dispatching Model is used to simulate public transportation that operates regularly. 

  7. Dynamics of a stellar bar

    International Nuclear Information System (INIS)

    Miller, R.H.; Smith, B.F.

    1979-01-01

    The dynamical properties of a prolate bar have been studied by means of a three-dimensional computer model. The bar pattern rotates in the sense of the total angular momentum. The mean particle motion is a rapid streaming in the direction of pattern rotation as seen from a frame that rotates with the bar. Rotation rates that would be inferred from observation are significantly (2--3 times) faster than the pattern rotation speed. Velocity dispersions are anisotropic with the largest component along the bar. Particles oscillate in the bar potential significantly faster than pattern rotation: typical oscillation frequencies are around ω/sub z/=ω/sub y/=6Ω and ω/sub x/=3Ω where z is the direction of angular momentum, x lies along the bar, and Ω is the pattern angular velocity. About 25% of the star orbits are near 2:2:1 resonance with the slow motion along the bar. Particle motion is highly ordered in the bar:the ratio t=T/sub mean//vertical-barWvertical-bar is 0.21--0.24. Observable properties are described; where comparisons can be made, observable properties are in agreement with observations of brightness contours, velocity fields, and velocity dispersions. The bar has nearly exponential density profiles

  8. Variation in alternative splicing across human tissues

    OpenAIRE

    Yeo, Gene; Holste, Dirk; Kreiman, Gabriel; Burge, Christopher B

    2004-01-01

    Background: Alternative pre-mRNA splicing (AS) is widely used by higher eukaryotes to generate different protein isoforms in specific cell or tissue types. To compare AS events across human tissues, we analyzed the splicing patterns of genomically aligned expressed sequence tags (ESTs) derived from libraries of cDNAs from different tissues. Results: Controlling for differences in EST coverage among tissues, we found that the brain and testis had the highest levels of exon skipping. The most p...

  9. Thermopriming Triggers Splicing Memory in Arabidopsis

    KAUST Repository

    Ling, Yu

    2018-02-20

    Abiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred to as priming, can allow plants to survive subsequent and otherwise lethal conditions; the priming effect persists even after a prolonged stress-free period. However, the molecular mechanisms underlying priming are not fully understood. Here, we investigated the molecular basis of heat shock memory and the role of priming in Arabidopsisthaliana. Comprehensive analysis of transcriptome-wide changes in gene expression and alternative splicing in primed and non-primed plants revealed that alternative splicing functions as a novel component of heat shock memory. We show that priming of plants with a non-lethal heat stress results in de-repression of splicing after a second exposure to heat stress. By contrast, non-primed plants showed significant repression of splicing. These observations link ‘splicing memory’ to the ability of plants to survive subsequent and otherwise lethal heat stress. This newly discovered priming-induced splicing memory may represent a general feature of heat stress responses in plants and other organisms as many of the key components of heat shock responses are conserved among eukaryotes. Furthermore, this finding could facilitate the development of novel approaches to improve plant survival under extreme heat stress.

  10. Shuttle bus services quality assessment Tangerang Selatan toward smart city

    Science.gov (United States)

    Fassa, Ferdinand; Sitorus, Fredy Jhon Philip; Adikesuma, Tri Nugraha

    2017-11-01

    Around the world, shuttle bus operation played the significant role to accommodate transportation for commuting bus passengers. Shuttle Bus services in cities are provided by various bus agencies with kinds of own specific purposes. For instance, at Tangerang Selatan, Indonesia, it was said that shuttle bus In Trans Bintaro is run and operated by private bus companies hire by Bintaro developer. The aim of this research is to identify factors of satisfaction of shuttle bus service in Kota Tangerang Selatan, Indonesia. Several factors are used to analyze sums of 20 parameters performance indicators of Shuttle Bus. A face to face interview using a questionnaire (N=200) was used to collect data on October and March 2017. Likert and diagram Cartesian were used to model the all the parameters. This research succeeded in finding some categories of Shuttle bus service attributes such as accessibility, comfort, and safety. Users agreed that eight indicators in shuttle bus have the excellent achievement, while three indicators on performance remain low and should receive more attention especially punctuality of the bus.

  11. Ukola Club. Bar americano

    Directory of Open Access Journals (Sweden)

    Azpiazu, J. R.

    1961-03-01

    Full Text Available En la calle de Serrano, aprovechando un semisótano dedicado a otro negocio anteriormente, se ha instalado un bar americano, de cuyo interior ofrecemos algunos pormenores. Se han cuidado, especialmente, las condiciones acústicas, resueltas por medio de un techo de escayola perforada, con vitrofib en su parte superior, y paredes de madera, que contribuyen a darle un ambiente cálido y acogedor. El soporte de hierro laminado existente en el centro del local, cuya supresión hubiera sido costosa, se ha revestido con lajas de mármol que le convierten en un elemento decorativo.

  12. Genetics of alternative splicing evolution during sunflower domestication.

    Science.gov (United States)

    Smith, Chris C R; Tittes, Silas; Mendieta, J Paul; Collier-Zans, Erin; Rowe, Heather C; Rieseberg, Loren H; Kane, Nolan C

    2018-06-11

    Alternative splicing enables organisms to produce the diversity of proteins necessary for multicellular life by using relatively few protein-coding genes. Although differences in splicing have been identified among divergent taxa, the shorter-term evolution of splicing is understudied. The origins of novel splice forms, and the contributions of alternative splicing to major evolutionary transitions, are largely unknown. This study used transcriptomes of wild and domesticated sunflowers to examine splice differentiation and regulation during domestication. We identified substantial splicing divergence between wild and domesticated sunflowers, mainly in the form of intron retention. Transcripts with divergent splicing were enriched for seed-development functions, suggesting that artificial selection impacted splicing patterns. Mapping of quantitative trait loci (QTLs) associated with 144 differential splicing cases revealed primarily trans -acting variation affecting splicing patterns. A large proportion of identified QTLs contain known spliceosome proteins and are associated with splicing variation in multiple genes. Examining a broader set of wild and domesticated sunflower genotypes revealed that most differential splicing patterns in domesticated sunflowers likely arose from standing variation in wild Helianthus annuus and gained frequency during the domestication process. However, several domesticate-associated splicing patterns appear to be introgressed from other Helianthus species. These results suggest that sunflower domestication involved selection on pleiotropic regulatory alleles. More generally, our findings indicate that substantial differences in isoform abundances arose rapidly during a recent evolutionary transition and appear to contribute to adaptation and population divergence.

  13. Alternative RNA splicing and gastric cancer.

    Science.gov (United States)

    Li, Ying; Yuan, Yuan

    2017-07-01

    Alternative splicing (AS) linked to diseases, especially to tumors. Recently, more and more studies focused on the relationship between AS and gastric cancer (GC). This review surveyed the hot topic from four aspects: First, the common types of AS in cancer, including exon skipping, intron retention, mutually exclusive exon, alternative 5 ' or 3' splice site, alternative first or last exon and alternative 3' untranslated regions. Second, basic mechanisms of AS and its relationship with cancer. RNA splicing in eukaryotes follows the GT-AG rule by both cis-elements and trans-acting factors regulatory. Through RNA splicing, different proteins with different forms and functions can be produced and may be associated with carcinogenesis. Third, AS types of GC-related genes and their splicing variants. In this paper, we listed 10 common genes with AS and illustrated its possible molecular mechanisms owing to genetic variation (mutation and /or polymorphism). Fourth, the splicing variants of GC-associated genes and gastric carcinogenesis, invasion and metastasis. Many studies have found that the different splicing variants of the same gene are differentially expressed in GC and its precancerous diseases, suggesting AS has important implications in GC development. Taking together, this review highlighted the role of AS and splicing variants in the process of GC. We hope that this is not only beneficial to advances in the study field of GC, but also can provide valuable information to other similar tumor research.Although we already know some gene splicing and splicing variants play an important role in the development of GC, but many phenomena and mechanisms are still unknown. For example, how the tumor microenvironment and signal transduction pathway effect the forming and function of AS? Unfortunately, this review did not cover the contents because the current study is limited. It is no doubt that clarifying the phenomena and mechanisms of these unknown may help to reveal

  14. HIV-1 splicing is controlled by local RNA structure and binding of splicing regulatory proteins at the major 5' splice site

    NARCIS (Netherlands)

    Mueller, Nancy; Berkhout, Ben; Das, Atze T.

    2015-01-01

    The 5' leader region of the human immunodeficiency virus 1 (HIV-1) RNA genome contains the major 5' splice site (ss) that is used in the production of the many spliced viral RNAs. This splice-donor (SD) region can fold into a stable stem-loop structure and the thermodynamic stability of this RNA

  15. Bar piezoelectric ceramic transformers.

    Science.gov (United States)

    Erhart, Jiří; Pulpan, Půlpán; Rusin, Luboš

    2013-07-01

    Bar-shaped piezoelectric ceramic transformers (PTs) working in the longitudinal vibration mode (k31 mode) were studied. Two types of the transformer were designed--one with the electrode divided into two segments of different length, and one with the electrodes divided into three symmetrical segments. Parameters of studied transformers such as efficiency, transformation ratio, and input and output impedances were measured. An analytical model was developed for PT parameter calculation for both two- and three-segment PTs. Neither type of bar PT exhibited very high efficiency (maximum 72% for three-segment PT design) at a relatively high transformation ratio (it is 4 for two-segment PT and 2 for three-segment PT at the fundamental resonance mode). The optimum resistive loads were 20 and 10 kΩ for two- and three-segment PT designs for the fundamental resonance, respectively, and about one order of magnitude smaller for the higher overtone (i.e., 2 kΩ and 500 Ω, respectively). The no-load transformation ratio was less than 27 (maximum for two-segment electrode PT design). The optimum input electrode aspect ratios (0.48 for three-segment PT and 0.63 for two-segment PT) were calculated numerically under no-load conditions.

  16. Vitamin D and alternative splicing of RNA.

    Science.gov (United States)

    Zhou, Rui; Chun, Rene F; Lisse, Thomas S; Garcia, Alejandro J; Xu, Jianzhong; Adams, John S; Hewison, Martin

    2015-04-01

    The active form of vitamin D (1α,25-dihydroxyvitamin D, 1,25(OH)2D) exerts its genomic effects via binding to a nuclear high-affinity vitamin D receptor (VDR). Recent deep sequencing analysis of VDR binding locations across the complete genome has significantly expanded our understanding of the actions of vitamin D and VDR on gene transcription. However, these studies have also promoted appreciation of the extra-transcriptional impact of vitamin D on gene expression. It is now clear that vitamin D interacts with the epigenome via effects on DNA methylation, histone acetylation, and microRNA generation to maintain normal biological functions. There is also increasing evidence that vitamin D can influence pre-mRNA constitutive splicing and alternative splicing, although the mechanism for this remains unclear. Pre-mRNA splicing has long been thought to be a post-transcription RNA processing event, but current data indicate that this occurs co-transcriptionally. Several steroid hormones have been recognized to coordinately control gene transcription and pre-mRNA splicing through the recruitment of nuclear receptor co-regulators that can both control gene transcription and splicing. The current review will discuss this concept with specific reference to vitamin D, and the potential role of heterogeneous nuclear ribonucleoprotein C (hnRNPC), a nuclear factor with an established function in RNA splicing. hnRNPC, has been shown to be involved in the VDR transcriptional complex as a vitamin D-response element-binding protein (VDRE-BP), and may act as a coupling factor linking VDR-directed gene transcription with RNA splicing. In this way hnRNPC may provide an additional mechanism for the fine-tuning of vitamin D-regulated target gene expression. This article is part of a Special Issue entitled '17th Vitamin D Workshop'. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

    Directory of Open Access Journals (Sweden)

    Elela Sherif

    2006-01-01

    Full Text Available Abstract Background We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins. In the present study, we have tested the inhibitory activity of RNA oligonucleotides carrying different tail structures. Results We show that an oligonucleotide with a 5' tail containing the human β-globin branch site sequence inhibits the use of the 5' splice site of Bcl-xL, albeit less efficiently than a tail containing binding sites for the hnRNP A1/A2 proteins. A branch site-containing tail positioned at the 3' end of the oligonucleotide also elicited splicing inhibition but not as efficiently as a 5' tail. The interfering activity of a 3' tail was improved by adding a 5' splice site sequence next to the branch site sequence. A 3' tail carrying a Y-shaped branch structure promoted similar splicing interference. The inclusion of branch site or 5' splice site sequences in the Y-shaped 3' tail further improved splicing inhibition. Conclusion Our in vitro results indicate that a variety of tail architectures can be used to elicit splicing interference at low nanomolar concentrations, thereby broadening the scope and the potential impact of this antisense technology.

  18. Homologous SV40 RNA trans-splicing: Special case or prime example of viral RNA trans-splicing?

    Directory of Open Access Journals (Sweden)

    Sushmita Poddar

    2014-06-01

    Full Text Available To date the Simian Virus 40 (SV40 is the only proven example of a virus that recruits the mechanism of RNA trans-splicing to diversify its sequences and gene products. Thereby, two identical viral transcripts are efficiently joined by homologous trans-splicing triggering the formation of a highly transforming 100 kDa super T antigen. Sequences of other viruses including HIV-1 and the human adenovirus type 5 were reported to be involved in heterologous trans-splicing towards cellular or viral sequences but the meaning of these events remains unclear. We computationally and experimentally investigated molecular features associated with viral RNA trans-splicing and identified a common pattern: Viral RNA trans-splicing occurs between strong cryptic or regular viral splice sites and strong regular or cryptic splice sites of the trans-splice partner sequences. The majority of these splice sites are supported by exonic splice enhancers. Splice sites that could compete with the trans-splicing sites for cis-splice reactions are weaker or inexistent. Finally, all but one of the trans-splice reactions seem to be facilitated by one or more complementary binding domains of 11 to 16 nucleotides in length which, however occur with a statistical probability close to one for the given length of the involved sequences. The chimeric RNAs generated via heterologous viral RNA trans-splicing either did not lead to fusion proteins or led to proteins of unknown function. Our data suggest that distinct viral RNAs are highly susceptible to trans-splicing and that heterologous viral trans-splicing, unlike homologous SV40 trans-splicing, represents a chance event.

  19. VME bus based microcomputer system boards

    International Nuclear Information System (INIS)

    Chandra, A.K.; Ganesh, G.; Mayya, Anuradha; Chachondia, A.S.; Premraj, M.K.

    1991-01-01

    Several operator information systems for nuclear plants has been developed in the Division and these have involved extensive use of microcomputer boards for achieving various functions. Standard VME bus based boards have been developed to provide the most used functions. These boards have been fabricated and tested and used in several systems including Channel Temperature Monitoring systems, Disturbance Recording Systems etc. and are also proposed to be used in additional systems under developement. The use of standard bus and boards provides considerable savings in engineering time, prototyping, testing and evaluation costs, and maintenance support. This report desribes the various boards developed and the functions available on each. (author). 4 refs., 11 figs., 3 appendixes

  20. K-bar-mesic nuclei

    International Nuclear Information System (INIS)

    Dote, Akinobu; Akaishi, Yoshinori; Yamazaki, Toshimitsu

    2005-01-01

    New nuclei 'K-bar-Mesic Nuclei' having the strangeness are described. At first it is shown that the strongly attractive nature of K-bar N interaction is reasoned inductively from consideration of the relation between Kaonic hydrogen atom and Λ (1405) which is an excited state of hyperon Λ. The K-bar N interactions are reviewed and summarized into three categories: 1. Phenomenological approach with density dependent K-bar N interaction (DD), relativistic mean field (RMF) approach, and hybrid of them (RMF+DD). 2. Boson exchange model. 3. Chiral SU(3) theory. The investigation of some light K-bar-nuclei by Akaishi and Yamazaki using phenomenological K-bar N interaction is explained in detail. Studies by antisymmetrized molecular dynamics (AMD) approach are also presented. From these theoretical researches, the following feature of K-bar-mesic nuclei are revealed: 1) Ground state is discrete and bound by 100 MeV or more. 2) Density is very high in side the K-bar-mesic nuclei. 3) Strange structures develop which are not seen in ordinary nuclei. Finally some recent experiments to explore K-bar-mesic nuclei are reviewed. (S. Funahashi)

  1. Barred spiral structure of galaxies

    International Nuclear Information System (INIS)

    Chen, Z.; Weng, s.; Xu, M.

    1982-01-01

    Observational data indicate the grand design of spiral or barred spiral structure in disk galaxies. The problem of spiral structure has been thoroughly investigated by C. C. Lin and his collaborators, but yet the problem of barred spiral structure has not been investigated systematically, although much work has been done, such as in Ref. 3--7. Using the gasdynamic model for galaxies and a method of integral transform presented in Ref. 1, we investigated the barred spiral structure and obtained an analytical solution. It gives the large-scale pattern of barred-spirals, which is in fairly good agreement with observational data

  2. Exposure Assessment of Diesel Bus Emissions

    Directory of Open Access Journals (Sweden)

    Werner Hofmann

    2006-12-01

    Full Text Available The goal of this study was to measure ultrafine particle concentrations with diameters less than 1 μm emitted by diesel buses and to assess resulting human exposure levels. The study was conducted at the Woolloongabba Busway station in Brisbane, Australia in the winter months of 2002 during which temperature inversions frequently occurred. Most buses that utilize the station are fuelled by diesel, the exhaust of which contains a significant quantity of particle matter. Passengers waiting at the station are exposed to these particles emitted from the buses. During the course of this study, passenger census was conducted, based on video surveillance, yielding person-by-person waiting time data. Furthermore, a bus census revealed accurate information about the total number of diesel versus Compressed Natural Gas (CNG powered buses. Background (outside of the bus station and platform measurements of ultrafine particulate number size distributions were made to determine ambient aerosol concentrations. Particle number exposure concentration ranges from 10 and 40 to 60% of bus related exhaust fumes. This changes dramatically when considering the particle mass exposure concentration, where most passengers are exposed to about 50 to 80% of exhaust fumes. The obtained data can be very significant for comparison with similar work of this type because it is shown in previous studies that exhaust emissions causes cancer in laboratory animals. It was assumed that significant differences between platform and background distributions were due to bus emissions which, combined with passenger waiting times, yielded an estimate of passenger exposure to ultrafine particles from diesel buses. From an exposure point of view, the Busway station analyzed resembles a street canyon. Although the detected exhaust particle concentration at the outbound platform is found to be in the picogram range, exposure increases with the time passengers spend on the platform

  3. Modelling reveals kinetic advantages of co-transcriptional splicing.

    Directory of Open Access Journals (Sweden)

    Stuart Aitken

    2011-10-01

    Full Text Available Messenger RNA splicing is an essential and complex process for the removal of intron sequences. Whereas the composition of the splicing machinery is mostly known, the kinetics of splicing, the catalytic activity of splicing factors and the interdependency of transcription, splicing and mRNA 3' end formation are less well understood. We propose a stochastic model of splicing kinetics that explains data obtained from high-resolution kinetic analyses of transcription, splicing and 3' end formation during induction of an intron-containing reporter gene in budding yeast. Modelling reveals co-transcriptional splicing to be the most probable and most efficient splicing pathway for the reporter transcripts, due in part to a positive feedback mechanism for co-transcriptional second step splicing. Model comparison is used to assess the alternative representations of reactions. Modelling also indicates the functional coupling of transcription and splicing, because both the rate of initiation of transcription and the probability that step one of splicing occurs co-transcriptionally are reduced, when the second step of splicing is abolished in a mutant reporter.

  4. Modelling reveals kinetic advantages of co-transcriptional splicing.

    Science.gov (United States)

    Aitken, Stuart; Alexander, Ross D; Beggs, Jean D

    2011-10-01

    Messenger RNA splicing is an essential and complex process for the removal of intron sequences. Whereas the composition of the splicing machinery is mostly known, the kinetics of splicing, the catalytic activity of splicing factors and the interdependency of transcription, splicing and mRNA 3' end formation are less well understood. We propose a stochastic model of splicing kinetics that explains data obtained from high-resolution kinetic analyses of transcription, splicing and 3' end formation during induction of an intron-containing reporter gene in budding yeast. Modelling reveals co-transcriptional splicing to be the most probable and most efficient splicing pathway for the reporter transcripts, due in part to a positive feedback mechanism for co-transcriptional second step splicing. Model comparison is used to assess the alternative representations of reactions. Modelling also indicates the functional coupling of transcription and splicing, because both the rate of initiation of transcription and the probability that step one of splicing occurs co-transcriptionally are reduced, when the second step of splicing is abolished in a mutant reporter.

  5. Terminals for Suburb Bus Transport in Bratislava

    Science.gov (United States)

    Schlosser, Tibor; Schlosser, Peter; Cápayová, Silvia; Hodáková, Dominika

    2017-10-01

    The main objective of this article is to describe the strategy for development of the public transport terminals in the city of Bratislava, Capital of Slovak Republic. The reason goes from the private operator Slovak Lines, who operates the suburb bus transport in the agglomeration of the city. For this operator was created a transport model, while placing emphasis on optimizing the compliance of suburban public transport with urban public transport in the city of Bratislava and evaluating the significance of the new Bus Station to be constructed at Mlynské Nivy - in a new down town centre of the city. The main issue is to ensure the best available offer of public transport (PT) to passengers in the Bratislava agglomeration. The subject of the study was oriented to specify and propose changes in the transport infrastructure and integrated public transport organisation on the area of the city in terms of the significant position of the new Mlynské Nivy Bus Station (MN BS), which is under preparation with realization in the year 2017.

  6. Brazilian hybrid electric fuel cell bus

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, P.E.V.; Carreira, E.S. [Coppe-Federal Univ. of Rio de Janeiro (Brazil). Hydrogen Lab.

    2010-07-01

    The first prototype of a hybrid electric fuel cell bus developed with Brazilian technology is unveiled. It is a 12 m urban-type, low-floor, air-conditioned bus that possesses three doors, air suspension, 29 seats and reversible wheelchair site. The bus body was built based on a double-deck type monoblock vehicle that is able to sustain important load on its roof. This allowed positioning of the type 3 hydrogen tanks and the low weight traction batteries on the roof of the vehicles without dynamic stabilization problems. A novel hybrid energy configuration was designed in such a way that the low-power (77 kWe) fuel cell works on steady-state operation mode, not responding directly to the traction motor load demand. The rate of kinetic energy regeneration upon breaking was optimized by the use of an electric hybrid system with predominance of batteries and also by utilizing supercapacitors. The electric-electronic devices and the security control softwares for the auxiliary and traction systems were developed in-house. The innovative hybrid-electric traction system configuration led to the possibility to decrease the fuel cell power, with positive impact on weight and system volume reduction, as well as to significantly decrease the hydrogen consumption. (orig.)

  7. The 20S proteasome splicing activity discovered by SpliceMet.

    Directory of Open Access Journals (Sweden)

    Juliane Liepe

    2010-06-01

    Full Text Available The identification of proteasome-generated spliced peptides (PSP revealed a new unpredicted activity of the major cellular protease. However, so far characterization of PSP was entirely dependent on the availability of patient-derived cytotoxic CD8+ T lymphocytes (CTL thus preventing a systematic investigation of proteasome-catalyzed peptide splicing (PCPS. For an unrestricted PSP identification we here developed SpliceMet, combining the computer-based algorithm ProteaJ with in vitro proteasomal degradation assays and mass spectrometry. By applying SpliceMet for the analysis of proteasomal processing products of four different substrate polypeptides, derived from human tumor as well as viral antigens, we identified fifteen new spliced peptides generated by PCPS either by cis or from two separate substrate molecules, i.e., by trans splicing. Our data suggest that 20S proteasomes represent a molecular machine that, due to its catalytic and structural properties, facilitates the generation of spliced peptides, thereby providing a pool of qualitatively new peptides from which functionally relevant products may be selected.

  8. Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni.

    Science.gov (United States)

    Boroni, Mariana; Sammeth, Michael; Gava, Sandra Grossi; Jorge, Natasha Andressa Nogueira; Macedo, Andréa Mara; Machado, Carlos Renato; Mourão, Marina Moraes; Franco, Glória Regina

    2018-03-01

    Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional classes, which underlines the SLTS as a ubiquitous mechanism in the parasite. Also, SLTS gene expression levels span several orders of magnitude, showing that SLTS frequency is not determined by the expression level of the target gene, but by the presence of particular gene features facilitating or hindering the trans-splicing mechanism. Our in-depth investigation of SLTS events demonstrates widespread alternative trans-splicing (ATS) acceptor sites occurring in different regions along the entire gene body, highlighting another important role of SLTS generating alternative RNA isoforms in the parasite, besides the polycistron resolution. Particularly for introns where SLTS directly competes for the same acceptor substrate with cis-splicing, we identified for the first time additional and important features that might determine the type of splicing. Our study substantially extends the current knowledge of RNA processing by SLTS in S. mansoni, and provide basis for future studies on the trans-splicing mechanism in other eukaryotes.

  9. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    Directory of Open Access Journals (Sweden)

    Penny David

    2007-10-01

    Full Text Available Abstract Background Alternative splicing has been reported in various eukaryotic groups including plants, apicomplexans, diatoms, amoebae, animals and fungi. However, whether widespread alternative splicing has evolved independently in the different eukaryotic groups or was inherited from their last common ancestor, and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional classes, cellular locations, intron/exon structures and evolutionary origins. Results For each species, we find that genes from most functional categories are alternatively spliced. Ancient genes (shared between animals, fungi and plants show high levels of alternative splicing. Genes with products expressed in the nucleus or plasma membrane are generally more alternatively spliced while those expressed in extracellular location show less alternative splicing. We find a clear correspondence between incidence of alternative splicing and intron number per gene both within and between genomes. In general, we find several similarities in patterns of alternative splicing across these diverse eukaryotes. Conclusion Along with previous studies indicating intron-rich genes with weak intron boundary consensus and complex spliceosomes in ancestral organisms, our results suggest that at least a simple form of alternative splicing may already have been present in the unicellular ancestor of plants, fungi and animals. A role for alternative splicing in the evolution of multicellularity then would largely have arisen by co-opting the preexisting process.

  10. Alternative splicing in cancers: From aberrant regulation to new therapeutics.

    Science.gov (United States)

    Song, Xiaowei; Zeng, Zhenyu; Wei, Huanhuan; Wang, Zefeng

    2018-03-01

    Alternative splicing is one of the most common mechanisms for gene regulation in humans, and plays a vital role to increase the complexity of functional proteins. In this article, we seek to provide a general review on the relationships between alternative splicing and tumorigenesis. We briefly introduce the basic rules for regulation of alternative splicing, and discuss recent advances on dynamic regulation of alternative splicing in cancers by highlighting the roles of a variety of RNA splicing factors in tumorigenesis. We further discuss several important questions regarding the splicing of long noncoding RNAs and back-splicing of circular RNAs in cancers. Finally, we discuss the current technologies that can be used to manipulate alternative splicing and serve as potential cancer treatment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Implementation of viscoelastic Hopkinson bars

    Directory of Open Access Journals (Sweden)

    Govender R.

    2012-08-01

    Full Text Available Knowledge of the properties of soft, viscoelastic materials at high strain rates are important in furthering our understanding of their role during blast or impact events. Testing these low impedance materials using a metallic split Hopkinson pressure bar setup results in poor signal to noise ratios due to impedance mismatching. These difficulties are overcome by using polymeric Hopkinson bars. Conventional Hopkinson bar analysis cannot be used on the polymeric bars due to the viscoelastic nature of the bar material. Implementing polymeric Hopkinson bars requires characterization of the viscoelastic properties of the material used. In this paper, 30 mm diameter Polymethyl Methacrylate bars are used as Hopkinson pressure bars. This testing technique is applied to polymeric foam called Divinycell H80 and H200. Although there is a large body of of literature containing compressive data, this rarely deals with strain rates above 250s−1 which becomes increasingly important when looking at the design of composite structures where energy absorption during impact events is high on the list of priorities. Testing of polymeric foams at high strain rates allows for the development of better constitutive models.

  12. Peptic ulcer among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1990-01-01

    As part of a survey on the work environment of bus drivers, 2045 (83%) of 2465 male bus drivers in the three major cities in Denmark in 1978 answered a postal questionnaire on health and working conditions. In order to evaluate the relative occurrence of peptic ulcer among the bus drivers, a follow......-up study was also conducted. All hospital discharges with a peptic ulcer diagnosis among the bus drivers were registered from the Danish National Patient Register. All Danish men were used as reference group. On the basis of the 1978-questionnaire association between occupational and psychosocial factors...... and subsequent hospital discharge with a peptic ulcer diagnosis was studied. The prevalence of abdominal pain alleviated by food intake was 12% among bus drivers and 6% in the reference group. The incidence of hospital discharge with duodenal ulcer among younger bus drivers was twice the incidence among Danish...

  13. SPA: a probabilistic algorithm for spliced alignment.

    Directory of Open Access Journals (Sweden)

    2006-04-01

    Full Text Available Recent large-scale cDNA sequencing efforts show that elaborate patterns of splice variation are responsible for much of the proteome diversity in higher eukaryotes. To obtain an accurate account of the repertoire of splice variants, and to gain insight into the mechanisms of alternative splicing, it is essential that cDNAs are very accurately mapped to their respective genomes. Currently available algorithms for cDNA-to-genome alignment do not reach the necessary level of accuracy because they use ad hoc scoring models that cannot correctly trade off the likelihoods of various sequencing errors against the probabilities of different gene structures. Here we develop a Bayesian probabilistic approach to cDNA-to-genome alignment. Gene structures are assigned prior probabilities based on the lengths of their introns and exons, and based on the sequences at their splice boundaries. A likelihood model for sequencing errors takes into account the rates at which misincorporation, as well as insertions and deletions of different lengths, occurs during sequencing. The parameters of both the prior and likelihood model can be automatically estimated from a set of cDNAs, thus enabling our method to adapt itself to different organisms and experimental procedures. We implemented our method in a fast cDNA-to-genome alignment program, SPA, and applied it to the FANTOM3 dataset of over 100,000 full-length mouse cDNAs and a dataset of over 20,000 full-length human cDNAs. Comparison with the results of four other mapping programs shows that SPA produces alignments of significantly higher quality. In particular, the quality of the SPA alignments near splice boundaries and SPA's mapping of the 5' and 3' ends of the cDNAs are highly improved, allowing for more accurate identification of transcript starts and ends, and accurate identification of subtle splice variations. Finally, our splice boundary analysis on the human dataset suggests the existence of a novel non

  14. Hereditary cancer genes are highly susceptible to splicing mutations

    Science.gov (United States)

    Soemedi, Rachel; Maguire, Samantha; Murray, Michael F.; Monaghan, Sean F.

    2018-01-01

    Substitutions that disrupt pre-mRNA splicing are a common cause of genetic disease. On average, 13.4% of all hereditary disease alleles are classified as splicing mutations mapping to the canonical 5′ and 3′ splice sites. However, splicing mutations present in exons and deeper intronic positions are vastly underreported. A recent re-analysis of coding mutations in exon 10 of the Lynch Syndrome gene, MLH1, revealed an extremely high rate (77%) of mutations that lead to defective splicing. This finding is confirmed by extending the sampling to five other exons in the MLH1 gene. Further analysis suggests a more general phenomenon of defective splicing driving Lynch Syndrome. Of the 36 mutations tested, 11 disrupted splicing. Furthermore, analyzing past reports suggest that MLH1 mutations in canonical splice sites also occupy a much higher fraction (36%) of total mutations than expected. When performing a comprehensive analysis of splicing mutations in human disease genes, we found that three main causal genes of Lynch Syndrome, MLH1, MSH2, and PMS2, belonged to a class of 86 disease genes which are enriched for splicing mutations. Other cancer genes were also enriched in the 86 susceptible genes. The enrichment of splicing mutations in hereditary cancers strongly argues for additional priority in interpreting clinical sequencing data in relation to cancer and splicing. PMID:29505604

  15. Hereditary cancer genes are highly susceptible to splicing mutations.

    Directory of Open Access Journals (Sweden)

    Christy L Rhine

    2018-03-01

    Full Text Available Substitutions that disrupt pre-mRNA splicing are a common cause of genetic disease. On average, 13.4% of all hereditary disease alleles are classified as splicing mutations mapping to the canonical 5' and 3' splice sites. However, splicing mutations present in exons and deeper intronic positions are vastly underreported. A recent re-analysis of coding mutations in exon 10 of the Lynch Syndrome gene, MLH1, revealed an extremely high rate (77% of mutations that lead to defective splicing. This finding is confirmed by extending the sampling to five other exons in the MLH1 gene. Further analysis suggests a more general phenomenon of defective splicing driving Lynch Syndrome. Of the 36 mutations tested, 11 disrupted splicing. Furthermore, analyzing past reports suggest that MLH1 mutations in canonical splice sites also occupy a much higher fraction (36% of total mutations than expected. When performing a comprehensive analysis of splicing mutations in human disease genes, we found that three main causal genes of Lynch Syndrome, MLH1, MSH2, and PMS2, belonged to a class of 86 disease genes which are enriched for splicing mutations. Other cancer genes were also enriched in the 86 susceptible genes. The enrichment of splicing mutations in hereditary cancers strongly argues for additional priority in interpreting clinical sequencing data in relation to cancer and splicing.

  16. Aspek Hukum Perlindungan terhadap Penumpang Bus dalam Mewujudkan Perlindungan Konsumen

    OpenAIRE

    Harahap, Rabiah Z

    2016-01-01

    Bus is the one of transportation which is often used by society. Society as passenger of bus righteously gains protection. Many of accident happens in public transportation especially bus that rises a question the important of protection for people as the accicent victim. This question is worthy to be solved because many of public transportation company ignores the passenger's right. Based on this, government shall take control with presentation of new regulation that aims protection people ...

  17. Emission inventory estimation of an intercity bus terminal.

    Science.gov (United States)

    Qiu, Zhaowen; Li, Xiaoxia; Hao, Yanzhao; Deng, Shunxi; Gao, H Oliver

    2016-06-01

    Intercity bus terminals are hotspots of air pollution due to concentrated activities of diesel buses. In order to evaluate the bus terminals' impact on air quality, it is necessary to estimate the associated mobile emission inventories. Since the vehicles' operating condition at the bus terminal varies significantly, conventional calculation of the emissions based on average emission factors suffers the loss of accuracy. In this study, we examined a typical intercity bus terminal-the Southern City Bus Station of Xi'an, China-using a multi-scale emission model-(US EPA's MOVES model)-to quantity the vehicle emission inventory. A representative operating cycle for buses within the station is constructed. The emission inventory was then estimated using detailed inputs including vehicle ages, operating speeds, operating schedules, and operating mode distribution, as well as meteorological data (temperature and humidity). Five functional areas (bus yard, platforms, disembarking area, bus travel routes within the station, and bus entrance/exit routes) at the terminal were identified, and the bus operation cycle was established using the micro-trip cycle construction method. Results of our case study showed that switching to compressed natural gas (CNG) from diesel fuel could reduce PM2.5 and CO emissions by 85.64 and 6.21 %, respectively, in the microenvironment of the bus terminal. When CNG is used, tail pipe exhaust PM2.5 emission is significantly reduced, even less than brake wear PM2.5. The estimated bus operating cycles can also offer researchers and policy makers important information for emission evaluation in the planning and design of any typical intercity bus terminals of a similar scale.

  18. Design Report for Isolated RS-485 Bus Node

    Science.gov (United States)

    2016-07-01

    TERMS Android , RS-485, isolated, USB, smartphone 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT UU 18. NUMBER...controlled wired RS-485 network. The Android -based smartphone or tablet is used in conjunction with a USB to serial bridge to operate as the bus master in...the system. The Android device operates in USB Host mode and communicates to the RS-485 bus as if a single peripheral on the USB bus. 15. SUBJECT

  19. Network Analysis of Urban Traffic with Big Bus Data

    OpenAIRE

    Zhao, Kai

    2016-01-01

    Urban traffic analysis is crucial for traffic forecasting systems, urban planning and, more recently, various mobile and network applications. In this paper, we analyse urban traffic with network and statistical methods. Our analysis is based on one big bus dataset containing 45 million bus arrival samples in Helsinki. We mainly address following questions: 1. How can we identify the areas that cause most of the traffic in the city? 2. Why there is a urban traffic? Is bus traffic a key cause ...

  20. Bar Coliseo, en Sevilla

    Directory of Open Access Journals (Sweden)

    de la Peña Neila, Antonio

    1963-10-01

    Full Text Available This bar is situated inside the «Coliseo» building, which houses a cinema, as well as a number of commercial establishments. In order not to break the unity of the total project, no attempt has been made to alter the exterior aspect of the bar. No attempt was made, either, to make it into an intimate, club type of bar, now so much in fashion. Rather has it been given a diaphanous style, seeking the best possible use of the floor space. The windows of the building are elongated, and there is an intermediate floor level, whose detailed structure is metallic. A cleverly designed staircase, of folded sheet metal connects the ground floor, the intermediate floor level and the restaurant. Materials were carefully chosen in accordance with their function. The colour scheme has a sustained unity throughout the building, and care has been taken to avoid surprising or vivid chromatic patterns. Ceramic enamels by the painter Santiago del Campo provide a feature of decoration on the ground floor, and also serve to cover up the return air ducts. On the top floor, the restaurant is fitted with coloured tile facings, the work of the Seville painters Maria Josefa Sánchez, María Dolores Sánchez and Emilio García Ortiz. The bottom joints of the timber beams, in conjunction with the tile patterns, is reminiscent of the traditional Sevillian habit of placing ceramic units between the timber framework of buildings. The initial problem of the architect was to combine the optimum functional efficiency and aesthetic quality of the project, and the final solution is undoubtedly successful.El establecimiento está situado dentro del edificio «Coliseo», complejo formado por una sala de cine, y con la parte lateral destinada a locales comerciales. Formando un conjunto único no se pensó nunca en transformar los revestimientos y molduras de fachada. Tampoco presidió la idea de conseguir un establecimiento íntimo «tipo Club», tan en boga actualmente, sino un

  1. An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly.

    Science.gov (United States)

    Philippe, Lucas; Pandarakalam, George C; Fasimoye, Rotimi; Harrison, Neale; Connolly, Bernadette; Pettitt, Jonathan; Müller, Berndt

    2017-08-21

    Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the precise details of SL trans-splicing have yet to be elucidated. In part, this is because the systematic identification of the molecules involved has not previously been possible due to the lack of a specific phenotype associated with defects in this process. We present here a novel GFP-based reporter assay that can monitor SL1 trans-splicing in living C. elegans. Using this assay, we have identified mutants in sna-1 that are defective in SL trans-splicing, and demonstrate that reducing function of SNA-1, SNA-2 and SUT-1, proteins that associate with SL1 RNA and related SmY RNAs, impairs SL trans-splicing. We further demonstrate that the Sm proteins and pICln, SMN and Gemin5, which are involved in small nuclear ribonucleoprotein assembly, have an important role in SL trans-splicing. Taken together these results provide the first in vivo evidence for proteins involved in SL trans-splicing, and indicate that continuous replacement of SL ribonucleoproteins consumed during trans-splicing reactions is essential for effective trans-splicing. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. Countermeasures for Developing New Energy Bus Standards in China

    Science.gov (United States)

    Shi, Xin

    2018-01-01

    With the rapid development of new energy vehicle technology, new energy bus has become more and more popular in China, and the relevant standards and policy are urgently needed to guide the market. According to the assessment of the development situation on new energy vehicle technology and new energy bus, combing with traffic policy guidance and the development trend of new energy vehicles, this paper aims to put forward the countermeasures of the new energy bus standard in China, including standard system, key standards and relevant recommendations. Research result is expected to provide decision support for the wide application of new energy bus in China.

  3. Status of the IEEE P896 Future Backplane Bus

    International Nuclear Information System (INIS)

    Gustavson, D.B.

    1983-10-01

    The IEEE P896 Future Backplane Bus project has been influenced by and has influenced FASTBUS and several other contemporary bus designs. This paper summarizes the current status of that project, which is directed toward the needs of modern 32-bit microprocessor systems with multiple processors. Some of the technology developed for P896 will be important for future non-ECL implementations of FASTBUS and other buses. In particular, new bus drivers and receivers should greatly improve the performance and reliability of backplane buses and cable buses. The current status of the P896 serial bus is also summarized

  4. The Software Bus, an Object-Oriented Data Exchange System

    International Nuclear Information System (INIS)

    Akerbaek, T.; Louka, M.

    1996-01-01

    This document describes the Software Bus System, developed for object-oriented task to task communication in a TCP/IP based network. The Software Bus is a set of library functions, developed to be used for the Picasso-3 UIMS, and as a general purpose tool for dynamically interfacing programs at run-time. The Software Bus offers a high level object-oriented data exchange mechanism that relieves the application programmer of the low level TCP/IP-programming and communication protocol handling. The Software Bus is currently available under several UNIX platforms and a version for Windows NT is planned for late 1996. (author)

  5. The hnRNP 2H9 gene, which is involved in the splicing reaction, is a multiply spliced gene

    DEFF Research Database (Denmark)

    Honoré, B

    2000-01-01

    The hnRNP 2H9 gene products are involved in the splicing process and participate in early heat shock-induced splicing arrest. By combining low/high stringency hybridisation, database search, Northern and Western blotting it is shown that the gene is alternatively spliced into at least six...

  6. The fitness cost of mis-splicing is the main determinant of alternative splicing patterns.

    Science.gov (United States)

    Saudemont, Baptiste; Popa, Alexandra; Parmley, Joanna L; Rocher, Vincent; Blugeon, Corinne; Necsulea, Anamaria; Meyer, Eric; Duret, Laurent

    2017-10-30

    Most eukaryotic genes are subject to alternative splicing (AS), which may contribute to the production of protein variants or to the regulation of gene expression via nonsense-mediated messenger RNA (mRNA) decay (NMD). However, a fraction of splice variants might correspond to spurious transcripts and the question of the relative proportion of splicing errors to functional splice variants remains highly debated. We propose a test to quantify the fraction of AS events corresponding to errors. This test is based on the fact that the fitness cost of splicing errors increases with the number of introns in a gene and with expression level. We analyzed the transcriptome of the intron-rich eukaryote Paramecium tetraurelia. We show that in both normal and in NMD-deficient cells, AS rates strongly decrease with increasing expression level and with increasing number of introns. This relationship is observed for AS events that are detectable by NMD as well as for those that are not, which invalidates the hypothesis of a link with the regulation of gene expression. Our results show that in genes with a median expression level, 92-98% of observed splice variants correspond to errors. We observed the same patterns in human transcriptomes and we further show that AS rates correlate with the fitness cost of splicing errors. These observations indicate that genes under weaker selective pressure accumulate more maladaptive substitutions and are more prone to splicing errors. Thus, to a large extent, patterns of gene expression variants simply reflect the balance between selection, mutation, and drift.

  7. Extracting potential bus lines of Customized City Bus Service based on public transport big data

    Science.gov (United States)

    Ren, Yibin; Chen, Ge; Han, Yong; Zheng, Huangcheng

    2016-11-01

    Customized City Bus Service (CCBS) can reduce the traffic congestion and environmental pollution that caused by the increasing in private cars, effectively. This study aims to extract the potential bus lines and each line's passenger density of CCBS by mining the public transport big data. The datasets used in this study are mainly Smart Card Data (SCD) and bus GPS data of Qingdao, China, from October 11th and November 7th 2015. Firstly, we compute the temporal-origin-destination (TOD) of passengers by mining SCD and bus GPS data. Compared with the traditional OD, TOD not only has the spatial location, but also contains the trip's boarding time. Secondly, based on the traditional DBSCAN algorithm, we put forwards an algorithm, named TOD-DBSCAN, combined with the spatial-temporal features of TOD.TOD-DBSCAN is used to cluster the TOD trajectories in peak hours of all working days. Then, we define two variables P and N to describe the possibility and passenger destiny of a potential CCBS line. P is the probability of the CCBS line. And N represents the potential passenger destiny of the line. Lastly, we visualize the potential CCBS lines extracted by our procedure on the map and analyse relationship between potential CCBS lines and the urban spatial structure.

  8. Bus Rapid Transit and Socioeconomic Condition of Bus Commuters in Lagos State

    Directory of Open Access Journals (Sweden)

    Owolabi L. Kuye

    2017-10-01

    Full Text Available Road transport is one of the most common means of transportation across the world. Bus transportation in Nigeria had witnessed many problems for the past decades considering the growing population with the attendant loss of lives and properties in most cities like Lagos in Nigeria. This paper focuses on the adequacy of services provided by Bus Rapid Transit (BRT in Lagos State with respect to the extent to which it influences the socioeconomic conditions of bus commuters. It examines the effect of services rendered by BRT on cost reduction potential, income and safety. Multistage sampling technique was used to select 20 samples each from all respondents in all local government area in Lagos State to reach a total of three hundred and thirty (330 samples. The data collection instrument was the questionnaire which was validated. Data collected were analysed with the use of descriptive statistics such as means, percentages and standard deviation and inferential statistics such as regression and analysis of variance techniques. Findings showed that BRT services has reduced the cost of bus commuters and also increased safety and security thereby increasing the socioeconomic condition of Lagosians. The study recommends improvements in BRT services through the deployment of technology as it had been used in developed countries.

  9. Capillary Electrophoresis Analysis of Conventional Splicing Assays

    DEFF Research Database (Denmark)

    de Garibay, Gorka Ruiz; Acedo, Alberto; García-Casado, Zaida

    2014-01-01

    of these assays is often challenging. Here, we explore this issue by conducting splicing assays in 31 BRCA2 genetic variants. All variants were assessed by RT-PCR followed by capillary electrophoresis and direct sequencing. If assays did not produce clear-cut outputs (Class-2 or Class-5 according to analytical...

  10. Oracle Service Bus 11g Development Cookbook

    CERN Document Server

    Schmutz, Guido; van Zoggel, Jan

    2012-01-01

    This cookbook is full of immediately useable recipes showing you how to develop service and message-oriented (integration) applications on the Oracle Service Bus. In addition to its cookbook style, which ensures the solutions are presented in a clear step-by-step manner, the explanations go into great detail, which makes it good learning material for everyone who has experience in OSB and wants to improve. Most of the recipes are designed in such a way that each recipe is presented as a separate, standalone entity and reading of prior recipes is not required. The finished solution of each reci

  11. Redesign of Transjakarta Bus Driver's Cabin

    Science.gov (United States)

    Mardi Safitri, Dian; Azmi, Nora; Singh, Gurbinder; Astuti, Pudji

    2016-02-01

    Ergonomic risk at work stations with type Seated Work Control was one of the problems faced by Transjakarta bus driver. Currently “Trisakti” type bus, one type of bus that is used by Transjakarta in corridor 9, serving route Pinang Ranti - Pluit, gained many complaints from drivers. From the results of Nordic Body Map questionnaires given to 30 drivers, it was known that drivers feel pain in the neck, arms, hips, and buttocks. Allegedly this was due to the seat position and the button/panel bus has a considerable distance range (1 meter) to be achieved by drivers. In addition, preliminary results of the questionnaire using Workstation Checklist identified their complaints about uncomfortable cushion, driver's seat backrest, and the exact position of the AC is above the driver head. To reduce the risk level of ergonomics, then did research to design the cabin by using a generic approach to designing products. The risk analysis driver posture before the design was done by using Rapid Upper Limb Assessment (RULA), Rapid Entire Body Assessment (REBA), and Quick Exposure Checklist (QEC), while the calculation of the moment the body is done by using software Mannequin Pro V10.2. Furthermore, the design of generic products was done through the stages: need metric-matrix, house of quality, anthropometric data collection, classification tree concept, concept screening, scoring concept, design and manufacture of products in the form of two-dimensional. While the design after design risk analysis driver posture was done by using RULA, REBA, and calculation of moments body as well as the design visualized using software 3DMax. From the results of analysis before the draft design improvements cabin RULA obtained scores of 6, REBA 9, and the result amounted to 57.38% QEC and moment forces on the back is 247.3 LbF.inch and on the right hip is 72.9 LbF.in. While the results of the proposed improvements cabin design RULA obtained scores of 3, REBA 4, and the moment of force on

  12. Development of an effective pinch bar

    CSIR Research Space (South Africa)

    Ottermann, RW

    2003-02-01

    Full Text Available . ....................................10 Figure 3-3: Layout of lightweight pinch bar extruded fibreglass tube. ..................................11 Figure 3-4: XDM lightweight pinch bar with manufactured glass fibre bar. ..........................12 Figure 3-5: XDM lightweight pinch... bar with extruded glass fibre tube. ................................12 Figure 3-6: Stiffness of a 2.8m lightweight pinch bar with an extruded glass fibre tube and a 25mm steel pinch bar...

  13. Low energy bar pp physics

    International Nuclear Information System (INIS)

    Amsler, C.; Crowe, K.

    1989-02-01

    A detailed investigation of proton-antiproton interactions at low energy has become feasible with the commissioning of the LEAR facility in 1983. We shall shortly review the status of bar pp annihilation at rest and the physics motivations for second generation experiments with the Crystal Barrel detector. This type of detector would be adequate for the study of both Kp and bar pp interactions on an extracted beam of the KAON Factory. We shall conclude with a few remarks on the physics opportunities with bar p's at the KAON Factory which, in our opinion, will not be covered by the present LEAR facility. 11 refs., 10 figs., 2 tabs

  14. A Conceptual Approach for Optimising Bus Stop Spacing

    Science.gov (United States)

    Johar, Amita; Jain, S. S.; Garg, P. k.

    2017-06-01

    An efficient public transportation system is essential of any country. The growth, development and shape of the urban areas are mainly due to availability of good transportation (Shah et al. in Inst Town Plan India J 5(3):50-59, 1). In developing countries, like India, travel by local bus in a city is very common. The accidents, congestion, pollution and appropriate location of bus stops are the major problems arising in metropolitan cities. Among all the metropolitan cities in India, Delhi has highest percentage of growth of population and vehicles. Therefore, it is important to adopt efficient and effective ways to improve mobility in different metropolitan cities in order to overcome the problem and to reduce the number of private vehicles on the road. The primary objective of this paper is to present a methodology for developing a model for optimum bus stop spacing (OBSS). It describes the evaluation of existing urban bus route, data collection, development of model for optimizing urban bus route and application of model. In this work, the bus passenger generalized cost method is used to optimize the spacing between bus stops. For the development of model, a computer program is required to be written. The applicability of the model has been evaluated by taking the data of urban bus route of Delhi Transport Corporation (DTC) in Excel sheet in first phase. Later on, it is proposed to develop a programming in C++ language. The developed model is expected to be useful to transport planner for rational design of the spacing of bus stops to save travel time and to generalize operating cost. After analysis it is found that spacing between the bus stop comes out to be between 250 and 500 m. The Proposed Spacing of bus stops is done considering the points that they don't come nearer to metro/rail station, entry or exit of flyover and near traffic signal.

  15. Possible heavy tetraquarks qQq-barQ-bar, qqQ-barQ-bar and qQQ-barQ-bar

    International Nuclear Information System (INIS)

    Cui Ying; Chen Xiaolin; Deng Weizhen; Zhu Shilin

    2007-01-01

    Assuming X(3872) is a qcq-barc-bar tetraquark and using its mass as input, the authors perform a schematic study of the masses of possible heavy tetraquarks using the color-magnetic interaction with the flavor symmetry breaking corrections. (authors)

  16. Widespread alternative and aberrant splicing revealed by lariat sequencing

    Science.gov (United States)

    Stepankiw, Nicholas; Raghavan, Madhura; Fogarty, Elizabeth A.; Grimson, Andrew; Pleiss, Jeffrey A.

    2015-01-01

    Alternative splicing is an important and ancient feature of eukaryotic gene structure, the existence of which has likely facilitated eukaryotic proteome expansions. Here, we have used intron lariat sequencing to generate a comprehensive profile of splicing events in Schizosaccharomyces pombe, amongst the simplest organisms that possess mammalian-like splice site degeneracy. We reveal an unprecedented level of alternative splicing, including alternative splice site selection for over half of all annotated introns, hundreds of novel exon-skipping events, and thousands of novel introns. Moreover, the frequency of these events is far higher than previous estimates, with alternative splice sites on average activated at ∼3% the rate of canonical sites. Although a subset of alternative sites are conserved in related species, implying functional potential, the majority are not detectably conserved. Interestingly, the rate of aberrant splicing is inversely related to expression level, with lowly expressed genes more prone to erroneous splicing. Although we validate many events with RNAseq, the proportion of alternative splicing discovered with lariat sequencing is far greater, a difference we attribute to preferential decay of aberrantly spliced transcripts. Together, these data suggest the spliceosome possesses far lower fidelity than previously appreciated, highlighting the potential contributions of alternative splicing in generating novel gene structures. PMID:26261211

  17. Language study on Spliced Semigraph using Folding techniques

    Science.gov (United States)

    Thiagarajan, K.; Padmashree, J.

    2018-04-01

    In this paper, we proposed algorithm to identify cut vertices and cut edges for n-Cut Spliced Semigraph and splicing the n-Cut Spliced Semigraph using cut vertices else cut edges or combination of cut vertex and cut edge and applying sequence of folding to the spliced semigraph to obtain the semigraph quadruple η(S)=(2, 1, 1, 1). We observed that the splicing and folding using both cut vertices and cut edges is applicable only for n-Cut Spliced Semigraph where n > 2. Also, we transformed the spliced semigraph into tree structure and studied the language for the semigraph with n+2 vertices and n+1 semivertices using Depth First Edge Sequence algorithm and obtain the language structure with sequence of alphabet ‘a’ and ‘b’.

  18. Schedule and complex motion of shuttle bus induced by periodic inflow of passengers

    Science.gov (United States)

    Nagatani, Takashi; Naito, Yuichi

    2011-09-01

    We have studied the dynamic behavior of a bus in the shuttle bus transportation with a periodic inflow. A bus schedule is closely related to the dynamics. We present the modified circle map model for the dynamics of the shuttle bus. The motion of the shuttle bus depends on the loading parameter and the inflow period. The shuttle bus displays the periodic, quasi-periodic, and chaotic motions with varying both loading parameter and inflow rate.

  19. Multiple splicing defects in an intronic false exon.

    Science.gov (United States)

    Sun, H; Chasin, L A

    2000-09-01

    Splice site consensus sequences alone are insufficient to dictate the recognition of real constitutive splice sites within the typically large transcripts of higher eukaryotes, and large numbers of pseudoexons flanked by pseudosplice sites with good matches to the consensus sequences can be easily designated. In an attempt to identify elements that prevent pseudoexon splicing, we have systematically altered known splicing signals, as well as immediately adjacent flanking sequences, of an arbitrarily chosen pseudoexon from intron 1 of the human hprt gene. The substitution of a 5' splice site that perfectly matches the 5' consensus combined with mutation to match the CAG/G sequence of the 3' consensus failed to get this model pseudoexon included as the central exon in a dhfr minigene context. Provision of a real 3' splice site and a consensus 5' splice site and removal of an upstream inhibitory sequence were necessary and sufficient to confer splicing on the pseudoexon. This activated context also supported the splicing of a second pseudoexon sequence containing no apparent enhancer. Thus, both the 5' splice site sequence and the polypyrimidine tract of the pseudoexon are defective despite their good agreement with the consensus. On the other hand, the pseudoexon body did not exert a negative influence on splicing. The introduction into the pseudoexon of a sequence selected for binding to ASF/SF2 or its replacement with beta-globin exon 2 only partially reversed the effect of the upstream negative element and the defective polypyrimidine tract. These results support the idea that exon-bridging enhancers are not a prerequisite for constitutive exon definition and suggest that intrinsically defective splice sites and negative elements play important roles in distinguishing the real splicing signal from the vast number of false splicing signals.

  20. The BaBar Mini

    International Nuclear Information System (INIS)

    Brown, David N.

    2003-01-01

    BaBar has recently deployed a new event data format referred to as the Mini. The mini uses efficient packing and aggressive noise suppression to represent the average reconstructed BaBar event in under 7 KBytes. The Mini packs detector information into simple transient data objects, which are then aggregated into roughly 10 composite persistent objects per event. The Mini currently uses Objectivity persistence, and it is being ported to use Root persistence. The Mini contains enough information to support detailed detector studies, while remaining small and fast enough to be used directly in physics analysis. Mini output is customizable, allowing users to both truncate unnecessary content or add content, depending on their needs. The Mini has now replaced three older formats as the primary output of BaBar event reconstruction. A reduced form of the Mini will soon replace the physics analysis format as well, giving BaBar a single, flexible event data format covering all its needs

  1. The BaBar mini

    International Nuclear Information System (INIS)

    Brown, David N.; BaBar Collaboration

    2003-01-01

    BaBar has recently deployed a new event data format referred to as the Mini. The mini uses efficient packing and aggressive noise suppression to represent the average reconstructed BaBar event in under 7 KBytes. The Mini packs detector information into simple transient data objects, which are then aggregated into roughly 10 composite persistent objects per event. The Mini currently uses Objectivity persistence, and it is being ported to use Root persistence. The Mini contains enough information to support detailed detector studies, while remaining small and fast enough to be used directly in physics analysis. Mini output is customizable, allowing users to both truncate unnecessary content or add content, depending on their needs. The Mini has now replaced three older formats as the primary output of BaBar event reconstruction. A reduced form of the Mini will soon replace the physics analysis format as well, giving BaBar a single, flexible event data format covering all its needs

  2. Illinois School Bus Driver Instructional Program. Trainee Guide. Revised.

    Science.gov (United States)

    Illinois State Board of Education, Springfield.

    This trainee guide contains six units of materials for use by those studying to become school bus drivers in the State of Illinois. Covered in the units are the following topics: school bus driver role and responsibility, passenger control, first aid, driving fundamentals, accidents and emergencies, and detecting hazards. Each unit contains a…

  3. Connecticut nutmeg fuel cell bus project : first analysis report.

    Science.gov (United States)

    2012-07-01

    This report summarizes the experience and early results from a fuel cell bus demonstration funded by the Federal Transit Administra-tion (FTA) under the National Fuel Cell Bus Program (NFCBP). A team led by the Northeast Advanced Vehicle Consortium a...

  4. Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus: Preliminary Evaluation Results

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K.; Eudy, L.

    2008-10-01

    This report provides preliminary results from a National Renewable Energy Laboratory evaluation of a protoptye fuel cell transit bus operating at Connecticut Transit in Hartford. Included are descriptions of the planned fuel cell bus demonstration and equipment; early results and agency experience are also provided.

  5. 3D reconstructions of a controlled bus bombing

    DEFF Research Database (Denmark)

    Villa, Chiara; Hansen, Nikolaj Friis; Hansen, Kamilla Maria

    2018-01-01

    Objectives: to demonstrate the usefulness of 3D reconstructions to better understand the dynamic of a controlled bus bombing. Materials and methods: 3D models of the victims (pigs) were created from post-mortem CT scanning using Mimic software; 3D models of the crime scene (bus) were generated by...

  6. Review of bus drivers' occupational stress and stress prevention

    NARCIS (Netherlands)

    Kompier, M.A.J.; Di Martino, V.

    1995-01-01

    Although heterogeneous in methodology and content, 32 studies from 13 countries on bus drivers' work and health are similar in their conclusions. Bus-driving–characterized by high demands, low control and low support – can be regarded as a classic example of high-strain occupation, with high risks

  7. 32 CFR 935.138 - Motor bus operation.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Motor bus operation. 935.138 Section 935.138 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND CODE Motor Vehicle Code § 935.138 Motor bus operation. Each person operating a motor...

  8. Bus Lifecycle Cost Model for Federal Land Management Agencies.

    Science.gov (United States)

    2011-09-30

    The Bus Lifecycle Cost Model is a spreadsheet-based planning tool that estimates capital, operating, and maintenance costs for various bus types over the full lifecycle of the vehicle. The model is based on a number of operating characteristics, incl...

  9. Triple bar, high efficiency mechanical sealer

    Science.gov (United States)

    Pak, Donald J.; Hawkins, Samantha A.; Young, John E.

    2013-03-19

    A clamp with a bottom clamp bar that has a planar upper surface is provided. The clamp may also include a top clamp bar connected to the bottom clamp bar, and a pressure distribution bar between the top clamp bar and the bottom clamp bar. The pressure distribution bar may have a planar lower surface in facing relation to the upper surface of the bottom clamp bar. An object is capable of being disposed in a clamping region between the upper surface and the lower surface. The width of the planar lower surface may be less than the width of the upper surface within the clamping region. Also, the pressure distribution bar may be capable of being urged away from the top clamp bar and towards the bottom clamp bar.

  10. The Human Splicing Factor ASF/SF2 can Specifically Recognize Pre-mRNA 5' Splice Sites

    Science.gov (United States)

    Zuo, Ping; Manley, James L.

    1994-04-01

    ASF/SF2 is a human protein previously shown to function in in vitro pre-mRNA splicing as an essential factor necessary for all splices and also as an alternative splicing factor, capable of switching selection of 5' splice sites. To begin to study the protein's mechanism of action, we have investigated the RNA binding properties of purified recombinant ASF/SF2. Using UV crosslinking and gel shift assays, we demonstrate that the RNA binding region of ASF/SF2 can interact with RNA in a sequence-specific manner, recognizing the 5' splice site in each of two different pre-mRNAs. Point mutations in the 5' splice site consensus can reduce binding by as much as a factor of 100, with the largest effects observed in competition assays. These findings support a model in which ASF/SF2 aids in the recognition of pre-mRNA 5' splice sites.

  11. Sustainable transport and the organisation of bus services in Manchester

    DEFF Research Database (Denmark)

    Gudmundsson, Henrik; Sørensen, Claus Hedegaard

    2008-01-01

    alternative modes of transport to private cars, such as public transport. In this paper we focus only on the potential of bus transport services in cities for attracting travellers from cars. It is a basic assumption behind this paper that the ability of public bus transport to attract travellers from cars...... depends on how the bus sector is organised. By organisation we adopt an institutional point of view and consider basic modes of governance. Basic modes of governance include the forms “market”, “hierarchy” and “network” (Powell 1990). So-called New Public Management (NPM) reforms have changed the modes...... of governance of bus transport in several countries, including Denmark and the United Kingdom. Our focus is on how New Public Mangement Reforms have changed the mix of governance modes and thus may have influenced the ability of urban bus transport to attract travellers from cars. We focus on a particular case...

  12. SISTEM RESERVASI TIKET BUS DI TERMINAL ARJOSARI MALANG

    Directory of Open Access Journals (Sweden)

    Surya Wirawan

    2013-10-01

    Full Text Available Bus Ticket Reservation System is an application that can be used to help booking bus tickets and the buyer will get a report via SMS Gateway. The reservation system is made by conducting field studies in Arjosari Bus Station Malang. This system uses SMS Gateway to send the report to the buyer after booking bus tickets online. SMS Gateway on this application serves as a liaison which delays sms between External Short Message entitiy (ESME and Short Message Service Center (SMSC and so does in reserve.The reservation system made is developed with the PHP programming language and has a prototype system pattern. System functions were tested using the validation testing, performance testing, and usability testing. The results percentage of responses usability testing is 67.7%. This shows that Ticket Reservation System at Arjosari Bus Station can be used well enough.

  13. Research on the Coordinated Design of Bus and Taxi Station

    Directory of Open Access Journals (Sweden)

    Jiangfeng Xi

    2015-01-01

    Full Text Available Rises in the number of transit buses, bus routes, and overall traffic volume in China’s cities, coupled with interference from other transport modes, such as taxis loading and unloading passengers nearby, have led to increasing traffic delays at bus stops, which is considered one of the factors degrading service levels and traffic operations on urban roadways. This paper studies traffic characteristics at bus stops, investigates variations in delay from different types or designs of bus stops, and analyzes the impact of it on traffic capacity, the purpose of which is to propose a solution to predicting the feasibility of an integrated design of bus stops and taxi stands with the help of mathematical models and based on the objectives of optimal traffic operations and passenger transfer.

  14. Improved design of HIRFL-CSR EVME bus controller

    International Nuclear Information System (INIS)

    Zhao Long; Liu Wufeng; Qiao Weimin; Jing Lan

    2009-01-01

    The EVME bus controller which is a key component of the HIRFL-CSR control system was improved. Besides reconfiguring the embedded Linux, a utility program was developed for data exchange between the controller and the database. The bus controller is based on ARM920T(ARM9) micro processor which is BGA packaged. The bus controller has the universal interface of VGA display, keyboard, and mouse. The backboard interface logic is programmed in an in-system configurable FPGA device. The bus can drive high current up to 64 mA, with the flexibility of the programmable signal definitions. All the improved performance helped the EVME bus controller play a crucial role in HIRFL-CSR control system. (authors)

  15. RELATING BOTTOM QUARK MASS IN DR-BAR AND MS-BAR REGULARIZATION SCHEMES

    International Nuclear Information System (INIS)

    2002-01-01

    The value of the bottom quark mass at Q = M Z in the (bar D)(bar R) scheme is an important input for the analysis of supersymmetric models with a large value of tan β. Conventionally, however, the running bottom quark mass extracted from experimental data is quoted in the (bar M)(bar S) scheme at the scale Q = m b . We describe a two loop procedure for the conversion of the bottom quark mass from (bar M)(bar S) to (bar D)(bar R) scheme. The Particle Data Group value m b # bar M# # bar S#(m b # bar M# # bar S#) = 4.2 ± 0.2 GeV corresponds to a range of 2.65-3.03 GeV for m b # bar D# # bar R#(M Z )

  16. Work fatigue in urban bus drivers

    Directory of Open Access Journals (Sweden)

    Teresa Makowiec-Dąbrowska

    2015-10-01

    Full Text Available Background: Bus drivers are a special group of professional drivers who are at a very high risk of fatigue. The aim of the study was to examine whether the driver’s subjective assessment of fatigue allows for the determination of its level and identification of its causes. Material and Methods: The study group comprised 45 randomly selected bus drivers (mean age – 43.7±7.9 years, period of employment as drivers – 14.7±8.6 years. Examinations were performed in all subjects four times – before and after work on the “easy” route (outside the city center, small traffic intensity and before and after work on the “difficult” route (city center, heavy traffic. The fatigue test questionnaire, based on the list of symptoms of fatigue prepared by the Japan Research Committee of Fatigue, was used in the study. Results: The rating of fatigue after the work was significantly higher than that before the work. The profile of fatigue after work was not influenced by the type of route, but the assessment of most symptoms of fatigue reached a higher level after the “difficult” routes and the differences were statistically significant for 7 symptoms. Only the ratings of leg fatigue, feeling of heaviness, and the necessity to squint eyes and gaze with effort reached the higher levels after driving the “easy” routes. It has been found that the level of fatigue was significantly correlated with the job characteristics (driving time, the length of the route, number of stops, etc. and with the abundance of food ingested and type of beverage (coffee vs. others drunk prior to driving. Conclusions: The questionnaire used in our study to assess the subjective feeling of fatigue has proved to be a sensitive and useful tool for indicating the level and causes of fatigue. The relationship between the symptoms of fatigue and the characteristics of job and lifestyle shows that actions must be taken by both the employers and employees to prevent fatigue

  17. Field observations of nearshore bar formation

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian

    2008-01-01

      The formation of an inner nearshore bar was observed during a high-energy event at the sandy beach of Vejers, Denmark. The bar accreted in situ during surf zone conditions and the growth of the bar was associated with the development of a trough landward of the bar. Measurements of hydrodynamics...

  18. The Wheels on the Bus Go “Buy Buy Buy”: School Bus Advertising Laws

    Science.gov (United States)

    2012-01-01

    School buses, a practical necessity for millions of children, are at the center of new efforts to raise revenue. School bus advertising laws bring public health and commercialization concerns to the school setting. In doing so, they potentially expose school districts to First Amendment lawsuits. I examined various school bus advertising bills and laws. I reviewed First Amendment “forum analysis” as applied in the transit and school settings to clarify how this legal test may affect school districts subject to such laws. I have made recommendations for school districts to enact appropriate policies to ensure that such advertising does not undermine public health and to enable the districts to maintain control over their property. PMID:22742065

  19. The wheels on the bus go "buy buy buy": school bus advertising laws.

    Science.gov (United States)

    Pomeranz, Jennifer L

    2012-09-01

    School buses, a practical necessity for millions of children, are at the center of new efforts to raise revenue. School bus advertising laws bring public health and commercialization concerns to the school setting. In doing so, they potentially expose school districts to First Amendment lawsuits. I examined various school bus advertising bills and laws. I reviewed First Amendment "forum analysis" as applied in the transit and school settings to clarify how this legal test may affect school districts subject to such laws. I have made recommendations for school districts to enact appropriate policies to ensure that such advertising does not undermine public health and to enable the districts to maintain control over their property.

  20. Entropic contributions to the splicing process

    International Nuclear Information System (INIS)

    Osella, Matteo; Caselle, Michele

    2009-01-01

    It has been recently argued that depletion attraction may play an important role in different aspects of cellular organization, ranging from the organization of transcriptional activity in transcription factories to the formation of nuclear bodies. In this paper, we suggest a new application of these ideas in the context of the splicing process, a crucial step of messenger RNA maturation in eukaryotes. We shall show that entropy effects and the resulting depletion attraction may explain the relevance of the aspecific intron length variable in the choice of splice-site recognition modality. On top of that, some qualitative features of the genome architecture of higher eukaryotes can find evolutionary realistic motivation in the light of our model

  1. Intravitreal Injection of Splice-switching Oligonucleotides to Manipulate Splicing in Retinal Cells

    Directory of Open Access Journals (Sweden)

    Xavier Gérard

    2015-01-01

    Full Text Available Leber congenital amaurosis is a severe hereditary retinal dystrophy responsible for neonatal blindness. The most common disease-causing mutation (c.2991+1655A>G; 10–15% creates a strong splice donor site that leads to insertion of a cryptic exon encoding a premature stop codon. Recently, we reported that splice-switching oligonucleotides (SSO allow skipping of the mutant cryptic exon and the restoration of ciliation in fibroblasts of affected patients, supporting the feasibility of a SSO-mediated exon skipping strategy to correct the aberrant splicing. Here, we present data in the wild-type mouse, which demonstrate that intravitreal administration of 2’-OMePS-SSO allows selective alteration of Cep290 splicing in retinal cells, including photoreceptors as shown by successful alteration of Abca4 splicing using the same approach. We show that both SSOs and Cep290 skipped mRNA were detectable for at least 1 month and that intravitreal administration of oligonucleotides did not provoke any serious adverse event. These data suggest that intravitreal injections of SSO should be considered to bypass protein truncation resulting from the c.2991+1655A>G mutation as well as other truncating mutations in genes which like CEP290 or ABCA4 have a mRNA size that exceed cargo capacities of US Food and Drug Administration (FDA-approved adeno-associated virus (AAV-vectors, thus hampering gene augmentation therapy.

  2. Resolving deconvolution ambiguity in gene alternative splicing

    Directory of Open Access Journals (Sweden)

    Hubbell Earl

    2009-08-01

    Full Text Available Abstract Background For many gene structures it is impossible to resolve intensity data uniquely to establish abundances of splice variants. This was empirically noted by Wang et al. in which it was called a "degeneracy problem". The ambiguity results from an ill-posed problem where additional information is needed in order to obtain an unique answer in splice variant deconvolution. Results In this paper, we analyze the situations under which the problem occurs and perform a rigorous mathematical study which gives necessary and sufficient conditions on how many and what type of constraints are needed to resolve all ambiguity. This analysis is generally applicable to matrix models of splice variants. We explore the proposal that probe sequence information may provide sufficient additional constraints to resolve real-world instances. However, probe behavior cannot be predicted with sufficient accuracy by any existing probe sequence model, and so we present a Bayesian framework for estimating variant abundances by incorporating the prediction uncertainty from the micro-model of probe responsiveness into the macro-model of probe intensities. Conclusion The matrix analysis of constraints provides a tool for detecting real-world instances in which additional constraints may be necessary to resolve splice variants. While purely mathematical constraints can be stated without error, real-world constraints may themselves be poorly resolved. Our Bayesian framework provides a generic solution to the problem of uniquely estimating transcript abundances given additional constraints that themselves may be uncertain, such as regression fit to probe sequence models. We demonstrate the efficacy of it by extensive simulations as well as various biological data.

  3. Assessing Bus Performance Rating in Kajang, Selangor

    Science.gov (United States)

    Norhisham, S.; Ismail, A.; Sidek, L. M.; Borhan, M. N.; Zaini, N.; Yen, A. T. G.

    2018-04-01

    The Transit Capacity and Quality of Service Manual (TCQSM) was chosen based on the review with four manuals and guidelines for the method will be applied. Six (6) attributes, namely Hours of Service, Passenger Load, Transit Auto Travel Time, Service Frequency, Punctuality Performance and Service Coverage were chosen for evaluation.[6][7][8]. A total of three (3) operators with seven (7) routes were identified in Kajang. Based on the final results, the Quality of Service (QOS) findings was rated as D, which was regarded as minimum or satisfactory. Most stakeholders’ recommendations were: for the operators to provide information on bus scheduling and have accurate information for the passengers. It was suggested that in future this study can be extended to all routes and could be repeated after the opening of the Mass Rapid Transit (MRT) stations in Kajang, so as to provide a comparison and better evaluation of the public buses in Kajang.

  4. Field bus technology in accelerator control systems

    International Nuclear Information System (INIS)

    Tang Shuming

    1999-01-01

    Since eighties to now, the computer technology, network communication and ULSI technology have been developing rapidly. The level of control for industries and scientific experiments has been upgraded accordingly, so as to meet the increasing requirements for automation. The control systems become more complicated; the devices in control systems become more and more intelligent. However the cost of DCS (Distributed Control System) is quite expensive and the period of system integration is very long. More than ten measurement results for two methods defined in the world, in order to get inter operability of intelligent devices and reduce the costs. The author presents the development trend of fieldbuses briefly and describes the main performances of CAN, LONWORKS, WOLDFIP and PROFIBUS which are mainly used in the world today. The author proposes that the field bus technology will be introduced into the accelerator control systems in the country

  5. Status of the FASTBUS standard data bus

    International Nuclear Information System (INIS)

    Larsen, R.S.

    1980-11-01

    FASTBUS is a new laboratory standard data bus intended for use in experimental data acquisition and control. The development of FASTBUS began with a feasibility study in 1976, and is presently at the development prototyping stage. The principal aims of the standard are to provide a factor of ten or more improvement in speed of data transfers over present systems, as well as to provide an architecture for systems containing multiple processors. Developmental prototypes now in progress include crates, backplanes, cooling devices, power supplies, test and diagnostic modules, and user modules. A sizeable software development effort is also underway. Several experiments have made commitments to use FASTBUS. A review of current work and potential applications is given. 10 figures

  6. Evolution of alternative splicing regulation: changes in predicted exonic splicing regulators are not associated with changes in alternative splicing levels in primates

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Roy, Scott William

    2009-01-01

    and changes in alternative splicing levels. This observation holds across different ESR exon positions, exon lengths, and 5' splice site strengths. We suggest that this lack of association is mainly due to the great importance of context for ESR functionality: many ESR-like motifs in primates may have little...

  7. A study of alternative splicing in the pig

    Directory of Open Access Journals (Sweden)

    Jørgensen Claus B

    2010-05-01

    Full Text Available Abstract Background Since at least half of the genes in mammalian genomes are subjected to alternative splicing, alternative pre-mRNA splicing plays an important contribution to the complexity of the mammalian proteome. Expressed sequence tags (ESTs provide evidence of a great number of possible alternative isoforms. With the EST resource for the domestic pig now containing more than one million porcine ESTs, it is possible to identify alternative splice forms of the individual transcripts in this species from the EST data with some confidence. Results The pig EST data generated by the Sino-Danish Pig Genome project has been assembled with publicly available ESTs and made available in the PigEST database. Using the Distiller package 2,515 EST clusters with candidate alternative isoforms were identified in the EST data with high confidence. In agreement with general observations in human and mouse, we find putative splice variants in about 30% of the contigs with more than 50 ESTs. Based on the criteria that a minimum of two EST sequences confirmed each splice event, a list of 100 genes with the most distinct tissue-specific alternative splice events was generated from the list of candidates. To confirm the tissue specificity of the splice events, 10 genes with functional annotation were randomly selected from which 16 individual splice events were chosen for experimental verification by quantitative PCR (qPCR. Six genes were shown to have tissue specific alternatively spliced transcripts with expression patterns matching those of the EST data. The remaining four genes had tissue-restricted expression of alternative spliced transcripts. Five out of the 16 splice events that were experimentally verified were found to be putative pig specific. Conclusions In accordance with human and rodent studies we estimate that approximately 30% of the porcine genes undergo alternative splicing. We found a good correlation between EST predicted tissue

  8. Measurement of the asymmetry parameter for the decay $\\bar\\Lambda \\to \\bar p\\pi^+$

    OpenAIRE

    BES collaboration

    2009-01-01

    Based on a sample of $58\\times10^6J/\\psi$ decays collected with the BESII detector at the BEPC, the $\\bar\\Lambda$ decay parameter $\\alpha_{\\bar\\Lambda}$ for $\\bar\\Lambda\\to \\bar p \\pi^+$ is measured using about 9000 $J/\\psi\\to\\Lambda\\bar\\Lambda\\to p \\bar p \\pi^+\\pi^-$ decays. A fit to the joint angular distributions yields $\\alpha_{\\bar\\Lambda}(\\bar\\Lambda\\to \\bar p\\pi^+)=-0.755\\pm0.083\\pm0.063$, where the first error is statistical, and the second systematic.

  9. Project survey for trolley-bus transport; Johdinautoliikenteen hankeselvitys

    Energy Technology Data Exchange (ETDEWEB)

    Anttila, T.; Eklund, P.; Airaksinen, S.; Maettoe, R.; Laehdetie, A.

    2011-04-15

    To investigate the feasibility and rationality of the revival of trolley bus traffic in Helsinki a full size project plan was conducted for a variety of alternatives. This included a survey on the latest developments of the trolley bus technology, three optional plans for route network, a budgetary level design for a fully functional depot in Koskela bus depot, examination of urban landscape structures, a study on organisational options, particular technical specifications applicable on suitable trolley buses and an examination of the impacts on the environment and on the future development of public transport. The basic concept of a trolley bus as a means of electric public transport vehicle has found its form over the years, but all the components of the system undergo at present intensive development. In context of this survey also the option of a fully autonomous electric bus powered by accumulators or fuel cells and the like was investigated. However, such technology is still totally inadequate for a heavy duty bus. There are no economically competitive and effective systems available as yet. The trolley bu is the far most reliable system and gives the best life cycle economy for a foreseeable future. Earliest estimates for alternative electric technologies in regular bus service are viable just after one investment cycle some time beyond 2030. Then it should be possible to run without the support of a comprehensive overhead line. The latest achievements in the development of trolley bus are auxiliary engine and accumulator systems allowing for more autonomous propulsion. Nowadays, trolley buses are equipped with an auxiliary power unit that alleviates the traditional restrictions of the overhead line. The modern trolley bus may run short distances without the trolley lines. Recuperation of the kinetic energy at deceleration (braking) either into the overhead line network or by storing it in the vehicle is one of the characteristics of modern trolley buses

  10. Task Analysis Assessment on Intrastate Bus Traffic Controllers

    Science.gov (United States)

    Yen Bin, Teo; Azlis-Sani, Jalil; Nur Annuar Mohd Yunos, Muhammad; Ismail, S. M. Sabri S. M.; Tajedi, Noor Aqilah Ahmad

    2016-11-01

    Public transportation acts as social mobility and caters the daily needs of the society for passengers to travel from one place to another. This is true for a country like Malaysia where international trade has been growing significantly over the past few decades. Task analysis assessment was conducted with the consideration of cognitive ergonomic view towards problem related to human factors. Conducting research regarding the task analysis on bus traffic controllers had allowed a better understanding regarding the nature of work and the overall monitoring activities of the bus services. This paper served to study the task analysis assessment on intrastate bus traffic controllers and the objectives of this study include to conduct task analysis assessment on the bus traffic controllers. Task analysis assessment for the bus traffic controllers was developed via Hierarchical Task Analysis (HTA). There are a total of five subsidiary tasks on level one and only two were able to be further broken down in level two. Development of HTA allowed a better understanding regarding the work and this could further ease the evaluation of the tasks conducted by the bus traffic controllers. Thus, human error could be reduced for the safety of all passengers and increase the overall efficiency of the system. Besides, it could assist in improving the operation of the bus traffic controllers by modelling or synthesizing the existing tasks if necessary.

  11. Accumulation of GC donor splice signals in mammals

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2008-07-01

    Full Text Available Abstract The GT dinucleotide in the first two intron positions is the most conserved element of the U2 donor splice signals. However, in a small fraction of donor sites, GT is replaced by GC. A substantial enrichment of GC in donor sites of alternatively spliced genes has been observed previously in human, nematode and Arabidopsis, suggesting that GC signals are important for regulation of alternative splicing. We used parsimony analysis to reconstruct evolution of donor splice sites and inferred 298 GT > GC conversion events compared to 40 GC > GT conversion events in primate and rodent genomes. Thus, there was substantive accumulation of GC donor splice sites during the evolution of mammals. Accumulation of GC sites might have been driven by selection for alternative splicing. Reviewers This article was reviewed by Jerzy Jurka and Anton Nekrutenko. For the full reviews, please go to the Reviewers' Reports section.

  12. Approaches to link RNA secondary structures with splicing regulation

    DEFF Research Database (Denmark)

    Plass, Mireya; Eyras, Eduardo

    2014-01-01

    In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by facilitat...... describes the steps in the analysis of the secondary structure of the pre-mRNA and its possible relation to splicing. As a working example, we use the case of yeast and the problem of the recognition of the 3' splice site (3'ss).......In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either...

  13. Splicing modulation therapy in the treatment of genetic diseases

    Directory of Open Access Journals (Sweden)

    Arechavala-Gomeza V

    2014-12-01

    Full Text Available Virginia Arechavala-Gomeza,1 Bernard Khoo,2 Annemieke Aartsma-Rus3 1Neuromuscular Disorders Group, BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain; 2Endocrinology, Division of Medicine, University College London, London, UK; 3Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands All authors contributed equally to this manuscript Abstract: Antisense-mediated splicing modulation is a tool that can be exploited in several ways to provide a potential therapy for rare genetic diseases. This approach is currently being tested in clinical trials for Duchenne muscular dystrophy and spinal muscular atrophy. The present review outlines the versatility of the approach to correct cryptic splicing, modulate alternative splicing, restore the open reading frame, and induce protein knockdown, providing examples of each. Finally, we outline a possible path forward toward the clinical application of this approach for a wide variety of inherited rare diseases. Keywords: splicing, therapy, antisense oligonucleotides, cryptic splicing, alternative splicing

  14. The emerging role of alternative splicing in senescence and aging.

    Science.gov (United States)

    Deschênes, Mathieu; Chabot, Benoit

    2017-10-01

    Deregulation of precursor mRNA splicing is associated with many illnesses and has been linked to age-related chronic diseases. Here we review recent progress documenting how defects in the machinery that performs intron removal and controls splice site selection contribute to cellular senescence and organismal aging. We discuss the functional association linking p53, IGF-1, SIRT1, and ING-1 splice variants with senescence and aging, and review a selection of splicing defects occurring in accelerated aging (progeria), vascular aging, and Alzheimer's disease. Overall, it is becoming increasingly clear that changes in the activity of splicing factors and in the production of key splice variants can impact cellular senescence and the aging phenotype. © 2017 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  15. Swisstrolley 3 - An articulated trolley bus; SWISSTROLLEY 3. Gelenktrolleybus

    Energy Technology Data Exchange (ETDEWEB)

    Gisler, H.-J.

    2006-07-01

    This illustrated final report for the Swiss Federal Office of Energy (SFOE) takes a look at the Swisstrolley 3 low-floor, articulated trolley bus developed in Switzerland by the Hess AG company. Various features of the variants of the trolley bus, including single vehicle, single and double-articulated buses and bus trailers, are discussed. The reductions achieved in energy consumption, weight and noise are discussed. Typical values for power consumption are presented in graphical form. Economic viability and passenger-friendliness are examined, as are possibilities for further improvement.

  16. Using microcomputers for lighting appliance control using a DALI bus

    Directory of Open Access Journals (Sweden)

    Sysala Tomas

    2016-01-01

    Full Text Available Article concerns the DALI bus theoretical and practical aspects. The DALI bus is a modern technology developed especially for illumination systems control. Authors are outlining communication basic principles related to the physical layer and going up to more complex data transactions among particular devices. The article main topic is aimed at two device models development based on microcontroller and exploiting that technology. The first of those two devices is a DALI slave unit enabling the light source or multi switch with light indication components control via DALI bus. The second device measures the ambient illumination intensity and it also detects errors in the load.

  17. Systematic Analysis of Splice-Site-Creating Mutations in Cancer

    Directory of Open Access Journals (Sweden)

    Reyka G. Jayasinghe

    2018-04-01

    Full Text Available Summary: For the past decade, cancer genomic studies have focused on mutations leading to splice-site disruption, overlooking those having splice-creating potential. Here, we applied a bioinformatic tool, MiSplice, for the large-scale discovery of splice-site-creating mutations (SCMs across 8,656 TCGA tumors. We report 1,964 originally mis-annotated mutations having clear evidence of creating alternative splice junctions. TP53 and GATA3 have 26 and 18 SCMs, respectively, and ATRX has 5 from lower-grade gliomas. Mutations in 11 genes, including PARP1, BRCA1, and BAP1, were experimentally validated for splice-site-creating function. Notably, we found that neoantigens induced by SCMs are likely several folds more immunogenic compared to missense mutations, exemplified by the recurrent GATA3 SCM. Further, high expression of PD-1 and PD-L1 was observed in tumors with SCMs, suggesting candidates for immune blockade therapy. Our work highlights the importance of integrating DNA and RNA data for understanding the functional and the clinical implications of mutations in human diseases. : Jayasinghe et al. identify nearly 2,000 splice-site-creating mutations (SCMs from over 8,000 tumor samples across 33 cancer types. They provide a more accurate interpretation of previously mis-annotated mutations, highlighting the importance of integrating data types to understand the functional and the clinical implications of splicing mutations in human disease. Keywords: splicing, RNA, mutations of clinical relevance

  18. Alternative Splicing as a Target for Cancer Treatment.

    Science.gov (United States)

    Martinez-Montiel, Nancy; Rosas-Murrieta, Nora Hilda; Anaya Ruiz, Maricruz; Monjaraz-Guzman, Eduardo; Martinez-Contreras, Rebeca

    2018-02-11

    Alternative splicing is a key mechanism determinant for gene expression in metazoan. During alternative splicing, non-coding sequences are removed to generate different mature messenger RNAs due to a combination of sequence elements and cellular factors that contribute to splicing regulation. A different combination of splicing sites, exonic or intronic sequences, mutually exclusive exons or retained introns could be selected during alternative splicing to generate different mature mRNAs that could in turn produce distinct protein products. Alternative splicing is the main source of protein diversity responsible for 90% of human gene expression, and it has recently become a hallmark for cancer with a full potential as a prognostic and therapeutic tool. Currently, more than 15,000 alternative splicing events have been associated to different aspects of cancer biology, including cell proliferation and invasion, apoptosis resistance and susceptibility to different chemotherapeutic drugs. Here, we present well established and newly discovered splicing events that occur in different cancer-related genes, their modification by several approaches and the current status of key tools developed to target alternative splicing with diagnostic and therapeutic purposes.

  19. Universal precision sine bar attachment

    Science.gov (United States)

    Mann, Franklin D. (Inventor)

    1989-01-01

    This invention relates to an attachment for a sine bar which can be used to perform measurements during lathe operations or other types of machining operations. The attachment can be used for setting precision angles on vises, dividing heads, rotary tables and angle plates. It can also be used in the inspection of machined parts, when close tolerances are required, and in the layout of precision hardware. The novelty of the invention is believed to reside in a specific versatile sine bar attachment for measuring a variety of angles on a number of different types of equipment.

  20. Star formation suppression and bar ages in nearby barred galaxies

    Science.gov (United States)

    James, P. A.; Percival, S. M.

    2018-03-01

    We present new spectroscopic data for 21 barred spiral galaxies, which we use to explore the effect of bars on disc star formation, and to place constraints on the characteristic lifetimes of bar episodes. The analysis centres on regions of heavily suppressed star formation activity, which we term `star formation deserts'. Long-slit optical spectroscopy is used to determine H β absorption strengths in these desert regions, and comparisons with theoretical stellar population models are used to determine the time since the last significant star formation activity, and hence the ages of the bars. We find typical ages of ˜1 Gyr, but with a broad range, much larger than would be expected from measurement errors alone, extending from ˜0.25 to >4 Gyr. Low-level residual star formation, or mixing of stars from outside the `desert' regions, could result in a doubling of these age estimates. The relatively young ages of the underlying populations coupled with the strong limits on the current star formation rule out a gradual exponential decline in activity, and hence support our assumption of an abrupt truncation event.

  1. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Penny, David

    2007-01-01

    , and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional......, we find several similarities in patterns of alternative splicing across these diverse eukaryotes. CONCLUSION: Along with previous studies indicating intron-rich genes with weak intron boundary consensus and complex spliceosomes in ancestral organisms, our results suggest that at least a simple form...... of alternative splicing may already have been present in the unicellular ancestor of plants, fungi and animals. A role for alternative splicing in the evolution of multicellularity then would largely have arisen by co-opting the preexisting process....

  2. Bar-spheroid interaction in galaxies

    Science.gov (United States)

    Hernquist, Lars; Weinberg, Martin D.

    1992-01-01

    N-body simulation and linear analysis is employed to investigate the secular evolution of barred galaxies, with emphasis on the interaction between bars and spheroidal components of galaxies. This interaction is argued to drive secular transfer of angular momentum from bars to spheroids, primarily through resonant coupling. A moderately strong bar, having mass within corotation about 0.3 times the enclosed spheroid mass, is predicted to shed all its angular momentum typically in less than about 10 exp 9 yr. Even shorter depletion time scales are found for relatively more massive bars. It is suggested either that spheroids around barred galaxies are structured so as to inhibit strong coupling with bars, or that bars can form by unknown processes long after disks are established. The present models reinforce the notion that bars can drive secular evolution in galaxies.

  3. Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process

    Directory of Open Access Journals (Sweden)

    Katja Meyer

    2015-07-01

    Full Text Available Alternative pre-messenger RNA splicing in higher plants emerges as an important layer of regulation upon exposure to exogenous and endogenous cues. Accordingly, mutants defective in RNA-binding proteins predicted to function in the splicing process show severe phenotypic alterations. Among those are developmental defects, impaired responses to pathogen threat or abiotic stress factors, and misregulation of the circadian timing system. A suite of splicing factors has been identified in the model plant Arabidopsis thaliana. Here we summarize recent insights on how defects in these splicing factors impair plant performance.

  4. Optimization Model for Headway of a Suburban Bus Route

    Directory of Open Access Journals (Sweden)

    Xiaohong Jiang

    2014-01-01

    Full Text Available Due to relatively low passenger demand, headways of suburban bus route are usually longer than those of urban bus route. Actually it is also difficult to balance the benefits between passengers and operators, subject to the service standards from the government. Hence the headway of a suburban bus route is usually determined on the empirical experience of transport planners. To cope with this problem, this paper proposes an optimization model for designing the headways of suburban bus routes by minimizing the operating and user costs. The user costs take into account both the waiting time cost and the crowding cost. The feasibility and validity of the proposed model are shown by applying it to the Route 206 in Jiangning district, Nanjing city of China. Weightages of passengers’ cost and operating cost are further discussed, considering different passenger flows. It is found that the headway and objective function are affected by the weightages largely.

  5. Global Optimization for Bus Line Timetable Setting Problem

    Directory of Open Access Journals (Sweden)

    Qun Chen

    2014-01-01

    Full Text Available This paper defines bus timetables setting problem during each time period divided in terms of passenger flow intensity; it is supposed that passengers evenly arrive and bus runs are set evenly; the problem is to determine bus runs assignment in each time period to minimize the total waiting time of passengers on platforms if the number of the total runs is known. For such a multistage decision problem, this paper designed a dynamic programming algorithm to solve it. Global optimization procedures using dynamic programming are developed. A numerical example about bus runs assignment optimization of a single line is given to demonstrate the efficiency of the proposed methodology, showing that optimizing buses’ departure time using dynamic programming can save computational time and find the global optimal solution.

  6. Large truck and bus crash facts, 2008. 

    Science.gov (United States)

    2010-03-01

    This annual edition of Large Truck and Bus Crash Facts contains descriptive statistics about fatal, injury, and : property damage only crashes involving large trucks and buses in 2008. Selected crash statistics on passenger : vehicles are also presen...

  7. CFD Investigation on Long-Haul Passenger Bus

    Science.gov (United States)

    Tan, C. F.; Tee, B. T.; Law, H. C.; Lim, T. L.

    2015-09-01

    Air flow distribution is one of the important factors that will influence the bus passenger comfort during long haul travel. Poor air flow distribution not only cause discomfort to the bus passenger but also influence their travel mode as well. The main purpose of this study is to investigate the air flow performance of the bus air-conditioning system through CFD simulation approach. A 3D CAD model of air ducts was drawn and hence analysed by using CFD software, namely ANSYS Fluent, to determine the airflow rate for every outlets of the air-conditioning system. The simulated result was then validated with experimental data obtained from prototype model of air duct. Based on the findings, new design concepts is proposed with the aim to meet the industry requirement as well as to improve the bus passenger comfort during long haul travel.

  8. Transit bus applications of lithium ion batteries : progress and prospects

    Science.gov (United States)

    2012-12-31

    This report provides an overview of diverse transit bus applications of advanced Lithium Ion Batteries (LIBs). The report highlights and illustrates several FTA programs that fostered the successful development, demonstration, and deployment of fuel-...

  9. Analysis of movable bus stop boarding and alighting areas.

    Science.gov (United States)

    2013-05-01

    This study explored the feasibility of using movable and reusable boarding and alighting (B&A) pads at bus stops. : Potential design alternatives in terms of materials and structural support for these pads were evaluated. The review : focused on the ...

  10. How to Reduce Those Costly School Bus Accidents.

    Science.gov (United States)

    Farmer, Ernest

    1985-01-01

    The deterrent to school bus accidents is preparedness. Training programs for drivers and mechanics, equipment specifications, and a community support base are some of the ways to prevent tragedy. (MLF)

  11. Southwest Direct Express Bus Demonstration in Orlando, FL

    Science.gov (United States)

    1988-04-01

    In August 1983, the Orange-Seminole-Osceola Transit Authority (OSOTA) initiated six express bus routes in the southwest corridor of Orlando (known collectively as the Southwest Direct) as an UMTA-funded demonstration project. While one objective of t...

  12. Understanding Factors of Distraction among Intrastate Bus Driver

    Directory of Open Access Journals (Sweden)

    Yunos Muhammad Nur Annuar Mohd

    2017-01-01

    Full Text Available The increase in number of the world population have affected the demand for public transport especially in larger cities. Intrastate buses are chosen as the main public transport by many people due to its affordable fares. Therefore, a better performance of the intrastate bus drivers would be significant to cater the high demand. Nevertheless, distraction among the intrastate bus drivers have been found as one of the major factors that could affect the performance of the drivers. Therefore, the objective of this paper is to provide a better understanding on the factors of distraction among intrastate bus drivers. This paper refers to findings from previous researches which are related to this field of study. In conclusion, this paper will provide a list of factors of distraction among the intrastate bus drivers which may degrade their performance.

  13. FTA fuel cell bus program : research accomplishments through 2011.

    Science.gov (United States)

    2012-03-01

    Prepared by the Federal Transit Administration (FTA) Office of Research, Demonstration, and Innovation (TRI), this report summarizes the accomplishments of fuel-cell-transit-bus-related research and demonstrations projects supported by FTA through 20...

  14. Modelling Field Bus Communications in Mixed-Signal Embedded Systems

    Directory of Open Access Journals (Sweden)

    Alassir Mohamad

    2008-01-01

    Full Text Available Abstract We present a modelling platform using the SystemC-AMS language to simulate field bus communications for embedded systems. Our platform includes the model of an I/O controller IP (in this specific case an C controller that interfaces a master microprocessor with its peripherals on the field bus. Our platform shows the execution of the embedded software and its analog response on the lines of the bus. Moreover, it also takes into account the influence of the circuits's I/O by including their IBIS models in the SystemC-AMS description, as well as the bus lines imperfections. Finally, we present simulation results to validate our platform and measure the overhead introduced by SystemC-AMS over a pure digital SystemC simulation.

  15. Modelling Field Bus Communications in Mixed-Signal Embedded Systems

    Directory of Open Access Journals (Sweden)

    Patrick Garda

    2008-08-01

    Full Text Available We present a modelling platform using the SystemC-AMS language to simulate field bus communications for embedded systems. Our platform includes the model of an I/O controller IP (in this specific case an I2C controller that interfaces a master microprocessor with its peripherals on the field bus. Our platform shows the execution of the embedded software and its analog response on the lines of the bus. Moreover, it also takes into account the influence of the circuits's I/O by including their IBIS models in the SystemC-AMS description, as well as the bus lines imperfections. Finally, we present simulation results to validate our platform and measure the overhead introduced by SystemC-AMS over a pure digital SystemC simulation.

  16. STRATEGI PENGEMBANGAN TRANSPORTASI WISATA KURA-KURA BUS DI BALI

    Directory of Open Access Journals (Sweden)

    Ayu Kusuma

    2018-01-01

    Full Text Available Kura-Kura Bus is one of tourist transportation companies in Bali with public shuttle bus service in tourism route. Data analysis in this research were using SWOT and AHP analysis, with participants who have position minimal as a supervisor. Participants selected based on their expertise and relevance with this article. Based on the results of SWOT and AHP analysis to develop tourist transportation is Kura-Kura Bus, which is considered the most realistic scenario is an optimistic scenario, with the goal to be a safe and comfortable transportation. To achieve the goal, based on priority strategies that could be done by management of Kura-Kura Bus are developing unique products, finding new markets, favoring public transport services, enchance brand reputation, developing smartphone application, and cross promotion with supplier.

  17. DIMETHYL ETHER (DME)-FUELED SHUTTLE BUS DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Elana M. Chapman; Shirish Bhide; Jennifer Stefanik; Howard Glunt; Andre L. Boehman; Allen Homan; David Klinikowski

    2003-04-01

    The objectives of this research and demonstration program are to convert a campus shuttle bus to operation on dimethyl ether, a potential ultra-clean alternative diesel fuel. To accomplish this objective, this project includes laboratory evaluation of a fuel conversion strategy, as well as, field demonstration of the DME-fueled shuttle bus. Since DME is a fuel with no lubricity (i.e., it does not possess the lubricating quality of diesel fuel), conventional fuel delivery and fuel injection systems are not compatible with dimethylether. Therefore, to operate a diesel engine on DME one must develop a fuel-tolerant injection system, or find a way to provide the necessary lubricity to the DME. In this project, they have chosen the latter strategy in order to achieve the objective with minimal need to modify the engine. The strategy is to blend DME with diesel fuel, to obtain the necessary lubricity to protect the fuel injection system and to achieve low emissions. The bulk of the efforts over the past year were focused on the conversion of the campus shuttle bus. This process, started in August 2001, took until April 2002 to complete. The process culminated in an event to celebrate the launching of the shuttle bus on DME-diesel operation on April 19, 2002. The design of the system on the shuttle bus was patterned after the system developed in the engine laboratory, but also was subjected to a rigorous failure modes effects analysis with help from Dr. James Hansel of Air Products. The result of this FMEA was the addition of layers of redundancy and over-pressure protection to the system on the shuttle bus. The system became operation in February 2002. Preliminary emissions tests and basic operation of the shuttle bus took place at the Pennsylvania Transportation institute's test track facility near the University Park airport. After modification and optimization of the system on the bus, operation on the campus shuttle route began in early June 2002. However, the

  18. Expandable antivibration bar for a steam generator

    International Nuclear Information System (INIS)

    Lagally, H.O.

    1986-01-01

    A steam generator tube support structure comprises expandable antivibration bars positioned between rows of tubes in the steam generator and attached to retaining rings surrounding the bundle of tubes. The antivibration bars have adjacent bar sections with mating surfaces formed as inclined planes which upon relative longitudinal motion between the upper and lower bars provides a means to increase the overall thickness across the structure to the required value. The bar section is retained against longitudinal movement in take-up assembly whereas the bar section is movable longitudinally by rotation of a nut. (author)

  19. Observations of offshore bar decay

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian

    2010-01-01

    the upper shoreface, and finally a stage of decaying bar form through loss of sediment volume at the outer boundary of the upper shoreface. The phenomenon has been previously documented in the Netherlands, the USA, the Canadian Great Lakes, and in New Zealand, but our present understanding...

  20. Qq(Q-bar)(q-bar)' states in chiral SU(3) quark model

    International Nuclear Information System (INIS)

    Zhang Haixia; Zhang Min; Zhang Zongye

    2007-01-01

    We study the masses of Qq(Q-bar)(q-bar)' states with J PC =0 ++ , 1 ++ , 1 +- and 2 ++ in the chiral SU(3) quark model, where Q is the heavy quark (c or b) and q(q') is the light quark (u,d or s). According to our numerical results, it is improbable to make the interpretation of [cn(c-bar)(n-bar)] 1 ++ and [cn(c-bar)(n-bar)] 2 ++ (n=u,d) states as X(3872) and Y(3940), respectively. However, it is interesting to find the tetraquarks in the bq(b-bar)(q-bar)' system. (authors)

  1. Scenario-based electric bus operation: A case study of Putrajaya, Malaysia

    OpenAIRE

    Lay Eng Teoh; Hooi Ling Khoo; Siew Yoke Goh; Lai Mun Chong

    2018-01-01

    Globally, transportation sector has emerged as one of the major sources of air pollution. Correspondingly, green mobility (with electric bus) is gaining increasing attention as an essential step to mitigate emission concern. As such, a proper electric bus network design and fleet planning is important especially for bus operator to acquire an adequate number of electric bus, right on time, in order to operate electric bus system viably. Thus, this paper aims to examine the possibility to oper...

  2. Regenerative Braking System for Series Hybrid Electric City Bus

    OpenAIRE

    Zhang, Junzhi; Lu, Xin; Xue, Junliang; Li, Bos

    2008-01-01

    Regenerative Braking Systems (RBS) provide an efficient method to assist hybrid electric buses achieve better fuel economy while lowering exhaust emissions. This paper describes the design and testing of three regenerative braking systems, one of which is a series regenerative braking system and two of which are parallel regenerative braking systems. The existing friction based Adjustable Braking System (ABS) on the bus is integrated with each of the new braking systems in order to ensure bus...

  3. Digital bus technology in new coal-fired plants

    Energy Technology Data Exchange (ETDEWEB)

    Blaney, J.; Murray, J. [Emerson Process Management (United States)

    2007-10-15

    The main issues associated with including digital bus technology such as Foundation fieldbus, Profibus-DP or DeviceNet, in a coal-fired power plant are deciding which systems to install and determining how to implement it. Although still new, digital bus experiences to date have shown that the technology performs solidly and when wiring best practices are followed a significantly shorted commissioning cycle can be achieved. 1 fig., 2 tabs.

  4. VHDL resolved function based inner communication bus for FPGA

    Science.gov (United States)

    Pozniak, Krzysztof T.

    2017-08-01

    This article discusses a method of building an internal, universal and parametric bus. The solution was designed for a variety of FPGA families and popular VHDL compilers. The algorithm of automatic configuration of address space and methods of receiving and sending addressed data are discussed. The basic solution realized in VHDL language in a behavioral form and chosen examples of practical use of the internal bus are presented in detail.

  5. Analysing passenger arrivals rates and waiting time at bus stops

    OpenAIRE

    Kaparias, I.; Rossetti, C.; Trozzi, V.

    2015-01-01

    The present study investigates the rather under-explored topic of passenger waiting times at public transport facilities. Using data collected from part of London’s bus network by means of physical counts, measurements and observations, and complemented by on-site passenger interviews, the waiting behaviour is analysed for a number of bus stops served by different numbers of lines. The analysis employs a wide range of statistical methods and tools, and concentrates on three aspects: passenger...

  6. The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

    NARCIS (Netherlands)

    Sugliani, M.; Brambilla, V.; Clerkx, E.J.M.; Koornneef, M.; Soppe, W.J.J.

    2010-01-01

    ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3- and ABI3-ß, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-ß transcript

  7. Functions for fission yeast splicing factors SpSlu7 and SpPrp18 in alternative splice-site choice and stress-specific regulated splicing.

    Directory of Open Access Journals (Sweden)

    Geetha Melangath

    Full Text Available Budding yeast spliceosomal factors ScSlu7 and ScPrp18 interact and mediate intron 3'ss choice during second step pre-mRNA splicing. The fission yeast genome with abundant multi-intronic transcripts, degenerate splice signals and SR proteins is an apt unicellular fungal model to deduce roles for core spliceosomal factors in alternative splice-site choice, intron retention and to study the cellular implications of regulated splicing. From our custom microarray data we deduce a stringent reproducible subset of S. pombe alternative events. We examined the role of factors SpSlu7 or SpPrp18 for these splice events and investigated the relationship to growth phase and stress. Wild-type log and stationary phase cells showed ats1+ exon 3 skipped and intron 3 retained transcripts. Interestingly the non-consensus 5'ss in ats1+ intron 3 caused SpSlu7 and SpPrp18 dependent intron retention. We validated the use of an alternative 5'ss in dtd1+ intron 1 and of an upstream alternative 3'ss in DUF3074 intron 1. The dtd1+ intron 1 non-canonical 5'ss yielded an alternative mRNA whose levels increased in stationary phase. Utilization of dtd1+ intron 1 sub-optimal 5' ss required functional SpPrp18 and SpSlu7 while compromise in SpSlu7 function alone hampered the selection of the DUF3074 intron 1 non canonical 3'ss. We analysed the relative abundance of these splice isoforms during mild thermal, oxidative and heavy metal stress and found stress-specific splice patterns for ats1+ and DUF3074 intron 1 some of which were SpSlu7 and SpPrp18 dependent. By studying ats1+ splice isoforms during compromised transcription elongation rates in wild-type, spslu7-2 and spprp18-5 mutant cells we found dynamic and intron context-specific effects in splice-site choice. Our work thus shows the combinatorial effects of splice site strength, core splicing factor functions and transcription elongation kinetics to dictate alternative splice patterns which in turn serve as an additional

  8. Effect of passenger position on fear of danger experienced during sudden bus stops.

    Science.gov (United States)

    Aoki, Takeo; Uetake, Teruo; Shimoda, Masahiro

    2009-06-01

    The purpose of this study was to identify the effect of bus passengers' positions on their fear of danger when a bus stopped suddenly. A temporary bus running course with one bus stop was set up on the campus of the Tokyo University of Agriculture and Technology (TUAT). The bus ran the course 14 times with the bus stopping twice during the course, once at the bus stop and again just after re-starting from the bus stop. The driver was asked to brake more strongly than usual when stopping. Sixteen students (15 males and 1 female) between the ages of 18 and 21 years participated. In turn, all participants were asked to take 14 different postures in the bus. Participants were also asked to report their level of fear on a rating scale each time the bus stopped. The study showed that (1) passengers' fear of danger at the first sudden stop was typically higher than that at the second stop, (2) standing passengers who held hand straps experienced more fear than those who held fixed safety devices, (3) bus passengers sitting on the centre of the rear seat had a great risk of injury if the bus stopped suddenly, and (4) when passengers faced the window and stood transversely with respect to from the moving direction of the bus and the bus stopped suddenly, passengers' fear of danger was affected by the side of the bus on which they stood as well as which hand they used to grasp a safety device.

  9. CROSS-FRONTIER PUBLIC TRANSPORT: Y BUS

    CERN Multimedia

    CERN Housing Service

    2002-01-01

    'Y' buses cover the journey between Val Thoiry in France (shopping centre including'Migros' and other stores) and Blandonnet in Switzerland, where they stop outside the 'Jumbo' shop, now renamed 'Carrefour'. In France, the buses connect up Thoiry, Sergy and St. Genis, then cross the border to stop outside CERN, Meyrin, before continuing towards Geneva along the 'Route de Meyrin', stopping outside the 'La Tour' hospital, then at the terminus Blandonnet. The normal fare on French territory with a slight incursion into Switzerland for this purpose as far as CERN, Meyrin, is 1.20 Euros per single journey (ticket valid for one hour). The same amount is charged for any single journey in Switzerland. For example, the cost of a single journey between St. Genis and the 'La Tour' hospital would be 2.40 Euros. The season ticket ('Abonnement de Travail') opens the right to purchase a ticket valid over any period of seven days which entitles the holder to as many journeys as required between the bus stop nearest to the pl...

  10. DIMETHYL ETHER (DME)-FUELED SHUTTLE BUS DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Elana M. Chapman; Shirish Bhide; Jennifer Stefanik; Howard Glunt; Andre L. Boehman; Allen Homan; David Klinikowski

    2003-04-01

    The objectives of this research and demonstration program are to convert a campus shuttle bus to operation on dimethyl ether, a potential ultra-clean alternative diesel fuel. To accomplish this objective, this project includes laboratory evaluation of a fuel conversion strategy, as well as, field demonstration of the DME-fueled shuttle bus. Since DME is a fuel with no lubricity (i.e., it does not possess the lubricating quality of diesel fuel), conventional fuel delivery and fuel injection systems are not compatible with dimethyl ether. Therefore, to operate a diesel engine on DME one must develop a fuel-tolerant injection system, or find a way to provide the necessary lubricity to the DME. In this project, they have chosen the latter strategy in order to achieve the objective with minimal need to modify the engine. Their strategy is to blend DME with diesel fuel, to obtain the necessary lubricity to protect the fuel injection system and to achieve low emissions. The bulk of the efforts over the past year were focused on the conversion of the campus shuttle bus. This process, started in August 2001, took until April 2002 to complete. The process culminated in an event to celebrate the launching of the shuttle bus on DME-diesel operation on April 19, 2002. The design of the system on the shuttle bus was patterned after the system developed in the engine laboratory, but also was subjected to a rigorous failure modes effects analysis (FMEA, referred to by Air Products as a ''HAZOP'' analysis) with help from Dr. James Hansel of Air Products. The result of this FMEA was the addition of layers of redundancy and over-pressure protection to the system on the shuttle bus. The system became operational in February 2002. Preliminary emissions tests and basic operation of the shuttle bus took place at the Pennsylvania Transportation Institute's test track facility near the University Park airport. After modification and optimization of the system on

  11. Environmental Assessment of Bus Transport in the Trondheim Region - Evaluation of Relevant Bus and Fuel Technologies and their Potential for Mitigating Emissions from Passenger Transportation

    OpenAIRE

    Buø, Tonje

    2015-01-01

    The overall aim of this thesis is to assess the carbon footprint of transport by bus in the Trondheim region. Bus transportation is promoted as a strategy both to combat local pollution problems in urban areas and to mitigate global greenhouse gas emissions from passenger transport. Still, the environmental impacts of bus transport have received fairly limited attention in research. The environmental impacts of bus transport are calculated through life cycle assessment. A model is develo...

  12. Splicing aberrations caused by constitutional RB1 gene mutations in ...

    Indian Academy of Sciences (India)

    in this family revealed skipping of exon 22 in three members of this family. In one proband, a ... This study reveals novel effects of RB1 mutations on splicing and suggests the utility of RNA analysis as an ... of life) and presence of multiple tumors (multifocal). The ..... spliced RNA have been linked to parent of origin as well as.

  13. Androgen Receptor Splice Variants and Resistance to Taxane Chemotherapy

    Science.gov (United States)

    2017-10-01

    resistant prostate cancer ; docetaxel; cabazitaxel; chemotherapy; androgen receptor splice variants; microtubule; ligand-binding domain; microtubule... receptor splice variants (AR-Vs) are associated with resistance to taxane chemotherapy in castration- resistant prostate cancer (CRPC). However, this...androgen receptor inhibitors in prostate cancer . Nat Rev Cancer . 2015;15:701–11.

  14. Revealing the Determinants of Widespread Alternative Splicing Perturbation in Cancer

    Directory of Open Access Journals (Sweden)

    Yongsheng Li

    2017-10-01

    Full Text Available It is increasingly appreciated that alternative splicing plays a key role in generating functional specificity and diversity in cancer. However, the mechanisms by which cancer mutations perturb splicing remain unknown. Here, we developed a network-based strategy, DrAS-Net, to investigate more than 2.5 million variants across cancer types and link somatic mutations with cancer-specific splicing events. We identified more than 40,000 driver variant candidates and their 80,000 putative splicing targets deregulated in 33 cancer types and inferred their functional impact. Strikingly, tumors with splicing perturbations show reduced expression of immune system-related genes and increased expression of cell proliferation markers. Tumors harboring different mutations in the same gene often exhibit distinct splicing perturbations. Further stratification of 10,000 patients based on their mutation-splicing relationships identifies subtypes with distinct clinical features, including survival rates. Our work reveals how single-nucleotide changes can alter the repertoires of splicing isoforms, providing insights into oncogenic mechanisms for precision medicine.

  15. Antitumorigenic potential of STAT3 alternative splicing modulation.

    Science.gov (United States)

    Zammarchi, Francesca; de Stanchina, Elisa; Bournazou, Eirini; Supakorndej, Teerawit; Martires, Kathryn; Riedel, Elyn; Corben, Adriana D; Bromberg, Jacqueline F; Cartegni, Luca

    2011-10-25

    Signal transducer and activator of transcription 3 (STAT3) plays a central role in the activation of multiple oncogenic pathways. Splicing variant STAT3β uses an alternative acceptor site within exon 23 that leads to a truncated isoform lacking the C-terminal transactivation domain. Depending on the context, STAT3β can act as a dominant-negative regulator of transcription and promote apoptosis. We show that modified antisense oligonucleotides targeted to a splicing enhancer that regulates STAT3 exon 23 alternative splicing specifically promote a shift of expression from STAT3α to STAT3β. Induction of endogenous STAT3β leads to apoptosis and cell-cycle arrest in cell lines with persistent STAT3 tyrosine phosphorylation compared with total STAT3 knockdown obtained by forced splicing-dependent nonsense-mediated decay (FSD-NMD). Comparison of the molecular effects of splicing redirection to STAT3 knockdown reveals a unique STAT3β signature, with a down-regulation of specific targets (including lens epithelium-derived growth factor, p300/CBP-associated factor, CyclinC, peroxisomal biogenesis factor 1, and STAT1β) distinct from canonical STAT3 targets typically associated with total STAT3 knockdown. Furthermore, similar in vivo redirection of STAT3 alternative splicing leads to tumor regression in a xenograft cancer model, demonstrating how pharmacological manipulation of a single key splicing event can manifest powerful antitumorigenic properties and validating endogenous splicing reprogramming as an effective cancer therapeutic approach.

  16. Quantitative regulation of alternative splicing in evolution and development

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Roy, Scott W

    2009-01-01

    Alternative splicing (AS) is a widespread mechanism with an important role in increasing transcriptome and proteome diversity by generating multiple different products from the same gene. Evolutionary studies of AS have focused primarily on the conservation of alternatively spliced sequences or o...

  17. Connecting the dots: chromatin and alternative splicing in EMT.

    Science.gov (United States)

    Warns, Jessica A; Davie, James R; Dhasarathy, Archana

    2016-02-01

    Nature has devised sophisticated cellular machinery to process mRNA transcripts produced by RNA Polymerase II, removing intronic regions and connecting exons together, to produce mature RNAs. This process, known as splicing, is very closely linked to transcription. Alternative splicing, or the ability to produce different combinations of exons that are spliced together from the same genomic template, is a fundamental means of regulating protein complexity. Similar to transcription, both constitutive and alternative splicing can be regulated by chromatin and its associated factors in response to various signal transduction pathways activated by external stimuli. This regulation can vary between different cell types, and interference with these pathways can lead to changes in splicing, often resulting in aberrant cellular states and disease. The epithelial to mesenchymal transition (EMT), which leads to cancer metastasis, is influenced by alternative splicing events of chromatin remodelers and epigenetic factors such as DNA methylation and non-coding RNAs. In this review, we will discuss the role of epigenetic factors including chromatin, chromatin remodelers, DNA methyltransferases, and microRNAs in the context of alternative splicing, and discuss their potential involvement in alternative splicing during the EMT process.

  18. Conservation and sex-specific splicing of the doublesex gene

    Indian Academy of Sciences (India)

    Genetic control of sex determination in insects has been best characterized in Drosophila melanogaster, where the master gene Sxl codes for RNA that is sex specifically spliced to produce a functional protein only in females. SXL regulates the sex-specific splicing of transformer (tra) RNA which, in turn, regulates the ...

  19. The implications of alternative splicing in the ENCODE protein complement

    DEFF Research Database (Denmark)

    Tress, Michael L.; Martelli, Pier Luigi; Frankish, Adam

    2007-01-01

    suggested as one explanation for the discrepancy between the number of human genes and functional complexity. Here, we carry out a detailed study of the alternatively spliced gene products annotated in the ENCODE pilot project. We find that alternative splicing in human genes is more frequent than has...

  20. Going Smokefree Matters - Bars and Restaurants Infographic

    Data.gov (United States)

    U.S. Department of Health & Human Services — Explore the Going Smokefree Matters – Bars and Restaurants Infographic which outlines key facts related to the effects of secondhand smoke exposure in bars and...

  1. Going Smokefree Matters - Bars and Restaurants Infographic

    Data.gov (United States)

    U.S. Department of Health & Human Services — Explore the Going Smokefree Matters – Bars and Restaurants Infographic which outlines key facts related to the effects of secondhand smoke exposure in bars and...

  2. An evaluation of a lane support system for bus rapid transit on narrow shoulders and the relation to bus driver mental workload

    NARCIS (Netherlands)

    Ward, Nicholas J.; Shankwitz, Craig; Gorgestani, Alec; Donath, Max; De Waard, Dick; Boer, Erwin R.

    2006-01-01

    The use of dedicated bus shoulders is a key method for implementing bus rapid transit (BRT) in areas that do not have the space for additional infrastructure. However, the narrow width of the bus shoulder and the need to anticipate traffic hazards in the adjacent lane can both be significant

  3. An Improved Model for Headway-Based Bus Service Unreliability Prevention with Vehicle Load Capacity Constraint at Bus Stops

    Directory of Open Access Journals (Sweden)

    Weiya Chen

    2012-01-01

    Full Text Available This paper presents an improved model for improving headway-based bus route service reliability at bus stops using real-time preventive operation control, taking into account dynamic interaction among random passenger demand, stochastic driving conditions of route segments, and vehicle load capacity constraint. In this model, the real-time information of passenger demand and vehicle operation is involved to predict the imminent unacceptable headway deviation, in the case of which some in-time preventive control strategies are deployed according to the given control rules. As a case study, a single fixed bus route with high-frequency services was simulated and different scenarios of real-time preventive operation control were performed. Headway adherence and average passenger wait time were used to measure bus service reliability. The results show that the improved model is closer to the real bus route service, and using real-time information to predict potential service unreliability and trigger in-time preventive control can reduce bus bunching and avoid big gap.

  4. CP asymmetries in B-bar → K-bar *( → K-bar π) l-bar l and untagged B-bar s, Bs → φ( → K+K-) l-bar l decays at NLO

    International Nuclear Information System (INIS)

    Bobeth, Christoph; Hiller, Gudrun; Piranishvili, Giorgi

    2008-01-01

    The decay B-bar → K-bar *( → K-bar π) l-bar l offers great opportunities to explore the physics at and above the electroweak scale by means of an angular analysis. We investigate the physics potential of the seven CP asymmetries plus the asymmetry in the rate, working at low dilepton mass using QCD factorization at next-to leading order (NLO). The b → s CP asymmetries are doubly Cabibbo-suppressed ∼ d , B d → K*( → K 0 π 0 ) l-bar l and B-bar s , B s → φ( → K + K - ) l-bar l decays. Analyses of these CP asymmetries can rule out, or further support the minimal description of CP violation through the CKM mechanism. Experimental studies are promising for (super) flavor factories and at hadron colliders.

  5. Alternative splicing of mutually exclusive exons--a review.

    Science.gov (United States)

    Pohl, Martin; Bortfeldt, Ralf H; Grützmann, Konrad; Schuster, Stefan

    2013-10-01

    Alternative splicing (AS) of pre-mRNAs in higher eukaryotes and several viruses is one major source of protein diversity. Usually, the following major subtypes of AS are distinguished: exon skipping, intron retention, and alternative 3' and 5' splice sites. Moreover, mutually exclusive exons (MXEs) represent a rare subtype. In the splicing of MXEs, two (or more) splicing events are not independent anymore, but are executed or disabled in a coordinated manner. In this review, several bioinformatics approaches for analyzing MXEs are presented and discussed. In particular, we revisit suitable definitions and nomenclatures, and bioinformatics tools for finding MXEs, adjacent and non-adjacent MXEs, clustered and grouped MXEs. Moreover, the molecular mechanisms for splicing MXEs proposed in the literature are reviewed and discussed. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  6. Splice Site Mutations in the ATP7A Gene

    DEFF Research Database (Denmark)

    Skjørringe, Tina; Tümer, Zeynep; Møller, Lisbeth Birk

    2011-01-01

    Menkes disease (MD) is caused by mutations in the ATP7A gene. We describe 33 novel splice site mutations detected in patients with MD or the milder phenotypic form, Occipital Horn Syndrome. We review these 33 mutations together with 28 previously published splice site mutations. We investigate 12...... mutations for their effect on the mRNA transcript in vivo. Transcriptional data from another 16 mutations were collected from the literature. The theoretical consequences of splice site mutations, predicted with the bioinformatics tool Human Splice Finder, were investigated and evaluated in relation...... to in vivo results. Ninety-six percent of the mutations identified in 45 patients with classical MD were predicted to have a significant effect on splicing, which concurs with the absence of any detectable wild-type transcript in all 19 patients investigated in vivo. Sixty-seven percent of the mutations...

  7. ANALISIS SARANA ANGKUTAN UMUM BUS DAMRI DI KOTA SEMARANG

    Directory of Open Access Journals (Sweden)

    Subhan Rizal Amin

    2015-12-01

    Full Text Available This study aims to analyze the availability and use of public transportation by taking a case study of the Damri bus which has Ngaliyan-Pucanggading route. The availability of public transport is not comparable to the demand for public transportation services so that the problems of transportation emerge. Additionally, the use of public transport becomes an important factor to develop the transportation. The study is conducted by surveying the Damri passengers of B.04 lane and logistic regression is used for the analysis. The method of determining the needs of transportation is issued by Departemen Perhubungan. It is used to calculate and determine whether the public transportation should be added or reduced. Then, the factors used as the independent variables in the analysis are the Damri’s safety, effectiveness, efficiency, service and reliability. The results show that Perum Damri does not need to add more Damri bus for B.04 because the results’ calculation is 0.6. and the factors that significantly influence the use Damri bus is the variable of safety, effectiveness and efficiency. However, the variables of service and reliability do not significantly influence the use of B.04 Damri bus.Penelitian ini bertujuan untuk menganalisis ketersediaan dan penggunaan angkutan sarana angkutan umum penumpang dengan mengambil studi kasus pada bus Damri jalur dengan trayek Ngaliyan-Pucanggading. Ketersediaan sarana angkutan umum yang tersedia tidak sebanding dengan besarnya permintaan akan jasa angkutan umum oleh masyarakat umum sehingga muncul permasalahan transportasi. Di samping itu, penggunaan angkutan umum yang beroperasi menjadi faktor penting dalam upaya pengembangan sarana transportasi. Penelitian ini dilakukan dengan menggunakan metode survey terhadap para penumpang bus Damri jalur B.04 dan dianalisis dengan Regresi Logistik. Metode penentuan kebutuhan armada dikeluarkan oleh Departemen Perhubungan yang digunakan untuk menghitung apakah suatu

  8. Iterated Local Search Algorithm with Strategic Oscillation for School Bus Routing Problem with Bus Stop Selection

    Directory of Open Access Journals (Sweden)

    Mohammad Saied Fallah Niasar

    2017-02-01

    Full Text Available he school bus routing problem (SBRP represents a variant of the well-known vehicle routing problem. The main goal of this study is to pick up students allocated to some bus stops and generate routes, including the selected stops, in order to carry students to school. In this paper, we have proposed a simple but effective metaheuristic approach that employs two features: first, it utilizes large neighborhood structures for a deeper exploration of the search space; second, the proposed heuristic executes an efficient transition between the feasible and infeasible portions of the search space. Exploration of the infeasible area is controlled by a dynamic penalty function to convert the unfeasible solution into a feasible one. Two metaheuristics, called N-ILS (a variant of the Nearest Neighbourhood with Iterated Local Search algorithm and I-ILS (a variant of Insertion with Iterated Local Search algorithm are proposed to solve SBRP. Our experimental procedure is based on the two data sets. The results show that N-ILS is able to obtain better solutions in shorter computing times. Additionally, N-ILS appears to be very competitive in comparison with the best existing metaheuristics suggested for SBRP

  9. 49 CFR 236.705 - Bar, locking.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Bar, locking. 236.705 Section 236.705..., MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.705 Bar, locking. A bar in an interlocking machine to which the locking dogs are attached. ...

  10. Bar code instrumentation for nuclear safeguards

    International Nuclear Information System (INIS)

    Bieber, A.M. Jr.

    1984-01-01

    This paper presents a brief overview of the basic principles of bar codes and the equipment used to make and to read bar code labels, and a summary of some of the more important factors that need to be considered in integrating bar codes into an information system

  11. N-barN interaction theoretical models

    International Nuclear Information System (INIS)

    Loiseau, B.

    1991-12-01

    In the framework of antinucleon-nucleon interaction theoretical models, our present understanding on the N-barN interaction is discussed, either from quark- or/and meson- and baryon-degrees of freedom, by considering the N-barN annihilation into mesons and the N-barN elastic and charge-exchange scattering. (author) 52 refs., 11 figs., 2 tabs

  12. Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells

    DEFF Research Database (Denmark)

    Bruun, Gitte H; Bang, Jeanne Mv; Christensen, Lise L

    2018-01-01

    designed splice switching oligonucleotides (SSO) that blocks the intronic hnRNP A1 binding site, and demonstrate that this completely rescues splicing of IKBKAP exon 20 in FD patient fibroblasts and increases the amounts of IKAP protein. We propose that this may be developed into a potential new specific...

  13. Schedule and complex motion of shuttle bus induced by periodic inflow of passengers

    International Nuclear Information System (INIS)

    Nagatani, Takashi; Naito, Yuichi

    2011-01-01

    We have studied the dynamic behavior of a bus in the shuttle bus transportation with a periodic inflow. A bus schedule is closely related to the dynamics. We present the modified circle map model for the dynamics of the shuttle bus. The motion of the shuttle bus depends on the loading parameter and the inflow period. The shuttle bus displays the periodic, quasi-periodic, and chaotic motions with varying both loading parameter and inflow rate. -- Highlights: → We studied the dynamic behavior of a bus in the shuttle bus transportation. → We presented the modified circle map model for the bus schedule. → We clarified the dependence of the tour time on both loading parameter and inflow period.

  14. Improvement of multiprocessing performance by using optical centralized shared bus

    Science.gov (United States)

    Han, Xuliang; Chen, Ray T.

    2004-06-01

    With the ever-increasing need to solve larger and more complex problems, multiprocessing is attracting more and more research efforts. One of the challenges facing the multiprocessor designers is to fulfill in an effective manner the communications among the processes running in parallel on multiple multiprocessors. The conventional electrical backplane bus provides narrow bandwidth as restricted by the physical limitations of electrical interconnects. In the electrical domain, in order to operate at high frequency, the backplane topology has been changed from the simple shared bus to the complicated switched medium. However, the switched medium is an indirect network. It cannot support multicast/broadcast as effectively as the shared bus. Besides the additional latency of going through the intermediate switching nodes, signal routing introduces substantial delay and considerable system complexity. Alternatively, optics has been well known for its interconnect capability. Therefore, it has become imperative to investigate how to improve multiprocessing performance by utilizing optical interconnects. From the implementation standpoint, the existing optical technologies still cannot fulfill the intelligent functions that a switch fabric should provide as effectively as their electronic counterparts. Thus, an innovative optical technology that can provide sufficient bandwidth capacity, while at the same time, retaining the essential merits of the shared bus topology, is highly desirable for the multiprocessing performance improvement. In this paper, the optical centralized shared bus is proposed for use in the multiprocessing systems. This novel optical interconnect architecture not only utilizes the beneficial characteristics of optics, but also retains the desirable properties of the shared bus topology. Meanwhile, from the architecture standpoint, it fits well in the centralized shared-memory multiprocessing scheme. Therefore, a smooth migration with substantial

  15. Determination of the quark coupling strength vertical bar V-ub vertical bar using baryonic decays

    NARCIS (Netherlands)

    Aaij, R.; Adeva, B.; Adinolfi, M.; Older, A. A.; Ajaltouni, Z.; Akar, S.; Albrecht, J.; Alessio, F.; Alexander, M.; Ali, S.; Alkhazov, G.; Cartelle, P. Alvarez; Alves, A. A.; Amato, S.; Amerio, S.; Amhis, Y.; An, L.; Anderlini, L.; Andreotti, M.; Andrews, J. E.; Appleby, R. B.; Gutierrez, O. Aquines; Archilli, F.; Artamonov, A.; Artuso, M.; Aslanides, E.; Auriemma, G.; Baalouch, M.; Bachmann, S.; Back, J. J.; Badalov, A.; Baesso, C.; Baldini, W.; Barlow, R. J.; Barschel, C.; Barsuk, S.; Barter, W.; Batozskaya, V.; Battista, V.; Beaucourt, L.; Beddow, J.; Bedeschi, F.; Bediaga, I.; Bel, L. J.; Belyaev, I.; Ben-Haim, E.; Bencivenni, G.; Onderwater, C. J. G.; Pellegrino, A.; Tolk, S.

    In the Standard Model of particle physics, the strength of the couplings of the b quark to the u and c quarks, vertical bar V-ub vertical bar and vertical bar V-ub vertical bar, are governed by the coupling of the quarks to the Higgs boson. Using data from the LHCb experiment at the Large Hadron

  16. Derivation of asymptotic Vertical BarΔIVertical Bar = 1/2 rule

    International Nuclear Information System (INIS)

    Terasaki, K.; Oneda, S.

    1982-01-01

    It is argued that the origin of the observed approximate Vertical BarΔIVertical Bar = 1/2 rule is the presence of an asymptotic Vertical BarΔIVertical Bar = 1/2 rule which exists among certain two-body hadronic weak matrix elements, involving especially the ground-state hadrons

  17. Scenario-based electric bus operation: A case study of Putrajaya, Malaysia

    Directory of Open Access Journals (Sweden)

    Lay Eng Teoh

    2018-03-01

    Full Text Available Globally, transportation sector has emerged as one of the major sources of air pollution. Correspondingly, green mobility (with electric bus is gaining increasing attention as an essential step to mitigate emission concern. As such, a proper electric bus network design and fleet planning is important especially for bus operator to acquire an adequate number of electric bus, right on time, in order to operate electric bus system viably. Thus, this paper aims to examine the possibility to operate electric bus as a replacement for the conventional bus operation (with natural gas buses for the study area in Putrajaya, Malaysia. In order to determine a proper-designed electric bus operating system in terms of electric bus route, service frequency and quantity, the proposed methodology is developed with the aid of a traffic modeling software to cater various scenarios. Based on the existing (conventional traffic and transit system in Putrajaya, the developed electric bus operating model is calibrated accordingly by considering various operational concerns including battery capacity and charging facility. The resultant findings revealed that the developed electric bus operating system in Putrajaya outperforms the conventional bus operation, not only in generating a higher profit margin for the bus operator, but also satisfying the passengers in a better manner (by carrying more passengers per unit of bus with a lower energy consumption.

  18. Production of n-bar's and Sigma-bar+-'s in e+e- annihilations

    International Nuclear Information System (INIS)

    Ferguson, T.; Buchanan, C.; Nodulman, L.; Poster, R.; Breidenbach, M.; Morehouse, C.C.; Vannucci, F.

    1979-01-01

    The production of antineutrons and charged Sigma-bar's in e + e - annihilations has been measured at √s +- production between 4 and 7 GeV is consistent with simple expectations for charmed-baryon production. A search for the decays Lambda-bar - /sub c/ → Sigma-bar +- π -+ π - and Sigma-baratsup asteriskat/sub c//Sigma-bar/sub c/ → Lambda-bar - /sub c/π +- yields no significant peaks. An upper limit, at the 90% confidence level, of sigmaatsub Lambda-baratc-italicB (Lambda-bar/sub c/ → Sigma-bar +- π -+ π - ) < 56 pb is set

  19. Galaxy Zoo: Observing secular evolution through bars

    International Nuclear Information System (INIS)

    Cheung, Edmond; Faber, S. M.; Koo, David C.; Athanassoula, E.; Bosma, A.; Masters, Karen L.; Nichol, Robert C.; Melvin, Thomas; Bell, Eric F.; Lintott, Chris; Schawinski, Kevin; Skibba, Ramin A.; Willett, Kyle W.

    2013-01-01

    In this paper, we use the Galaxy Zoo 2 data set to study the behavior of bars in disk galaxies as a function of specific star formation rate (SSFR) and bulge prominence. Our sample consists of 13,295 disk galaxies, with an overall (strong) bar fraction of 23.6% ± 0.4%, of which 1154 barred galaxies also have bar length (BL) measurements. These samples are the largest ever used to study the role of bars in galaxy evolution. We find that the likelihood of a galaxy hosting a bar is anticorrelated with SSFR, regardless of stellar mass or bulge prominence. We find that the trends of bar likelihood and BL with bulge prominence are bimodal with SSFR. We interpret these observations using state-of-the-art simulations of bar evolution that include live halos and the effects of gas and star formation. We suggest our observed trends of bar likelihood with SSFR are driven by the gas fraction of the disks, a factor demonstrated to significantly retard both bar formation and evolution in models. We interpret the bimodal relationship between bulge prominence and bar properties as being due to the complicated effects of classical bulges and central mass concentrations on bar evolution and also to the growth of disky pseudobulges by bar evolution. These results represent empirical evidence for secular evolution driven by bars in disk galaxies. This work suggests that bars are not stagnant structures within disk galaxies but are a critical evolutionary driver of their host galaxies in the local universe (z < 1).

  20. Bar Coding and Tracking in Pathology.

    Science.gov (United States)

    Hanna, Matthew G; Pantanowitz, Liron

    2016-03-01

    Bar coding and specimen tracking are intricately linked to pathology workflow and efficiency. In the pathology laboratory, bar coding facilitates many laboratory practices, including specimen tracking, automation, and quality management. Data obtained from bar coding can be used to identify, locate, standardize, and audit specimens to achieve maximal laboratory efficiency and patient safety. Variables that need to be considered when implementing and maintaining a bar coding and tracking system include assets to be labeled, bar code symbologies, hardware, software, workflow, and laboratory and information technology infrastructure as well as interoperability with the laboratory information system. This article addresses these issues, primarily focusing on surgical pathology. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Potential Influence of Metro on Bus: A Case Study

    Science.gov (United States)

    Selvakumar, M.; Abishek Reddy, M.; Sathish, V.; Venkatesh, R.

    2018-06-01

    A modal shift occurs when one mode of transport has a comparative advantage in a similar market over another. The present work concerns with the development of modal shift model for urban travel in Chennai, India. The modal shift model was calibrated using binary logit technique and validated using hold-out sample method. The validated model was used to predict the probability of shift in selected corridor. The recent introduction of metro rail in Chennai has lead to an increasing competition among public transport modes. To study the influence of metro on bus transport, a Stated Preference (SP) survey was conducted among express bus travellers. Using the SP survey data, a modal shift model was calibrated to estimate the plausible shift from bus to metro. Results indicate that variables like fare- difference, age, and income play an important role in the shift behaviour. When metro fare increases with respect to express bus fare, bus passengers are less willing to use metro and vice-versa.

  2. 1393 Ring Bus at JPL: Description and Status

    Science.gov (United States)

    Wysocky, Terry R.

    2007-01-01

    Completed Ring Bus IC V&V Phase - Ring Bus Test Plan Completed for SIM Project - Applicable to Other Projects Implemented a Avionics Bus Based upon the IEEE 1393 Standard - Excellent Starting Point for a General Purpose High-Speed Spacecraft Bus - Designed to Meet SIM Requirements for - Real-time deterministic, distributed systems. - Control system requirements - Fault detection and recovery Other JPL Projects Considering Implementation F'light Software Ring Bus Driver Module Began in 2006, Continues Participating in Standard Revision. Search for Earth-like planets orbiting nearby stars and measure the masses and orbits of the planets it finds. Survey 2000 nearby stars for planetary systems to learn whether our Solar System is unusual, or typical. Make a new catalog of star position 100 times more accurate than current measurements. Learn how our galaxy formed and will evolve by studying the dynamics of its stars. Critically test models of exactly how stars shine, including exotic objects like black holes, neutron stars and white dwarfs.

  3. A new MV bus transfer scheme for nuclear power plants

    International Nuclear Information System (INIS)

    Chang, C.K.

    2015-01-01

    The auxiliary power system of many generating stations consists of offsite power supply system and onsite power supply system, including emergency diesel generators (EDG) to provide secure power to auxiliary loads. If a normal power supply fails to supply power, then the power source is transferred to a standby power supply. In the case of nuclear power plants (NPP), the unit auxiliary transformer (UAT) and standby auxiliary transformer (SAT) - or station service transformer - are installed and powered from 2 offsite power circuits to meet regulatory requirements. The transfer methods of a motor bus from a normal source to a standby source used in power generating stations are fast bus transfer, in-phase transfer, or residual transfer. Fast bus transfer method is the most popular and residual voltage transfer method that is used as a backup in medium voltage buses in general. The use of the advanced technology like open circuit voltage prediction and digital signal processing algorithms can improve the reliability of fast transfer scheme. However, according to the survey results of the recent operation records in nuclear power plants, there were many instances where the fast transfer scheme has failed. To assure bus transfer in any conditions and circumstances, un-interruptible bus transfer scheme utilizing the state of the art medium voltage UPS (Un-interruptible Power Supply) is discussed and elaborated

  4. Bus network redesign for inner southeast suburbs of Melbourne, Australia

    Science.gov (United States)

    Pandangwati, S. T.; Milyanab, N. A.

    2017-06-01

    Public transport is the most effective mode of transport in the era of climate change and oil depletion. It can address climate change issues by reducing urban greenhouse gas emission and oil consumption while at the same time improving mobility. However, many public transport networks are not effective and instead create high operating costs with low frequencies and occupancy. Melbourne is one example of a metropolitan area that faces this problem. Even though the city has well-integrated train and tram networks, Melbourne’s bus network still needs to be improved. This study used network planning approach to redesign the bus network in the City of Glen Eira, a Local Government Area (LGA) in the southeastern part of Metropolitan Melbourne. The study area is the area between Gardenvale North and Oakleigh Station, as well as between Caulfield and Patterson Stations. This area needs network improvement mainly because of the meandering bus routes that run within it. This study aims to provide recommendations for improving the performance of bus services by reducing meandering routes, improving transfer point design and implementing coordinated timetables. The recommendations were formulated based on a ‘ready-made’ concept to increase bus occupancy. This approach can be implemented in other cities with similar problems and characteristics including those in Indonesia.

  5. Potential Influence of Metro on Bus: A Case Study

    Science.gov (United States)

    Selvakumar, M.; Abishek Reddy, M.; Sathish, V.; Venkatesh, R.

    2018-03-01

    A modal shift occurs when one mode of transport has a comparative advantage in a similar market over another. The present work concerns with the development of modal shift model for urban travel in Chennai, India. The modal shift model was calibrated using binary logit technique and validated using hold-out sample method. The validated model was used to predict the probability of shift in selected corridor. The recent introduction of metro rail in Chennai has lead to an increasing competition among public transport modes. To study the influence of metro on bus transport, a Stated Preference (SP) survey was conducted among express bus travellers. Using the SP survey data, a modal shift model was calibrated to estimate the plausible shift from bus to metro. Results indicate that variables like fare-difference, age, and income play an important role in the shift behaviour. When metro fare increases with respect to express bus fare, bus passengers are less willing to use metro and vice-versa.

  6. Low resistance splices for HTS devices and applications

    Science.gov (United States)

    Lalitha, S. L.

    2017-09-01

    This paper discusses the preparation methodology and performance evaluation of low resistance splices made of the second generation (2G) high-temperature superconductor (HTS). These splices are required in a broad spectrum of HTS devices including a large aperture, high-field solenoid built in the laboratory to demonstrate a superconducting magnetic energy storage (SMES) device. Several pancake coils are assembled in the form of a nested solenoid, and each coil requires a hundred meters or more of 2G (RE)BCO tape. However, commercial availability of this superconductor with a very uniform physical properties is currently limited to shorter piece lengths. This necessitates us having splices to inter-connect the tape pieces within a pancake coil, between adjacent pancake coils, and to attach HTS current leads to the magnet assembly. As a part of the optimization and qualification of splicing process, a systematic study was undertaken to analyze the electrical performance of splices in two different configurations suitable for this magnet assembly: lap joint and spiral joint. The electrical performance is quantified in terms of the resistance of splices estimated from the current-voltage characteristics. It has been demonstrated that a careful application of this splicing technique can generate lap joints with resistance less than 1 nΩ at 77 K.

  7. Aberrant and alternative splicing in skeletal system disease.

    Science.gov (United States)

    Fan, Xin; Tang, Liling

    2013-10-01

    The main function of skeletal system is to support the body and help movement. A variety of factors can lead to skeletal system disease, including age, exercise, and of course genetic makeup and expression. Pre-mRNA splicing plays a crucial role in gene expression, by creating multiple protein variants with different biological functions. The recent studies show that several skeletal system diseases are related to pre-mRNA splicing. This review focuses on the relationship between pre-mRNA splicing and skeletal system disease. On the one hand, splice site mutation that leads to aberrant splicing often causes genetic skeletal system disease, like COL1A1, SEDL and LRP5. On the other hand, alternative splicing without genomic mutation may generate some marker protein isoforms, for example, FN, VEGF and CD44. Therefore, understanding the relationship between pre-mRNA splicing and skeletal system disease will aid in uncovering the mechanism of disease and contribute to the future development of gene therapy. © 2013 Elsevier B.V. All rights reserved.

  8. Herboxidiene triggers splicing repression and abiotic stress responses in plants

    KAUST Repository

    Alshareef, Sahar

    2017-03-27

    Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.

  9. Study of the reactions $\\bar{p}p \\rightarrow \\bar{\\Lambda} \\Lambda , \\bar{\\Lambda} \\Sigma^{0}$ or $\\bar{\\Sigma^{0}} \\Lambda , \\bar{\\Sigma^{+}} \\Sigma^{+}$ at 3.6 GeV/c

    CERN Document Server

    Atherton, Henry W; Moebes, J P; Quercigh, Emanuele

    1974-01-01

    The reactions $\\bar{p}p \\rightarrow \\bar{\\Lambda} \\Lambda , \\bar{\\Lambda} \\Sigma^{0}$ or $\\bar{\\Sigma^{0}} \\Lambda , \\bar{\\Sigma^{+}} \\Sigma^{+}$ are studied at an incident momentum of 3.6 GeV/c in a 35.4 event/$\\mu$ b experiment performed in the CERN 2m HBC. Total and differential cross sections are presented. The polarization of the hyperons is measured as a function of $t$ and for the reaction $\\bar{p}p \\rightarrow \\bar{\\Lambda} \\Lambda$ the complete spin correlation matrix is given. (23 refs).

  10. Bar quenching in gas-rich galaxies

    Science.gov (United States)

    Khoperskov, S.; Haywood, M.; Di Matteo, P.; Lehnert, M. D.; Combes, F.

    2018-01-01

    Galaxy surveys have suggested that rapid and sustained decrease in the star-formation rate (SFR), "quenching", in massive disk galaxies is frequently related to the presence of a bar. Optical and near-IR observations reveal that nearly 60% of disk galaxies in the local universe are barred, thus it is important to understand the relationship between bars and star formation in disk galaxies. Recent observational results imply that the Milky Way quenched about 9-10 Gyr ago, at the transition between the cessation of the growth of the kinematically hot, old, metal-poor thick disk and the kinematically colder, younger, and more metal-rich thin disk. Although perhaps coincidental, the quenching episode could also be related to the formation of the bar. Indeed the transfer of energy from the large-scale shear induced by the bar to increasing turbulent energy could stabilize the gaseous disk against wide-spread star formation and quench the galaxy. To explore the relation between bar formation and star formation in gas rich galaxies quantitatively, we simulated gas-rich disk isolated galaxies. Our simulations include prescriptions for star formation, stellar feedback, and for regulating the multi-phase interstellar medium. We find that the action of stellar bar efficiently quenches star formation, reducing the star-formation rate by a factor of ten in less than 1 Gyr. Analytical and self-consistent galaxy simulations with bars suggest that the action of the stellar bar increases the gas random motions within the co-rotation radius of the bar. Indeed, we detect an increase in the gas velocity dispersion up to 20-35 km s-1 at the end of the bar formation phase. The star-formation efficiency decreases rapidly, and in all of our models, the bar quenches the star formation in the galaxy. The star-formation efficiency is much lower in simulated barred compared to unbarred galaxies and more rapid bar formation implies more rapid quenching.

  11. Diversification of the muscle proteome through alternative splicing.

    Science.gov (United States)

    Nakka, Kiran; Ghigna, Claudia; Gabellini, Davide; Dilworth, F Jeffrey

    2018-03-06

    Skeletal muscles express a highly specialized proteome that allows the metabolism of energy sources to mediate myofiber contraction. This muscle-specific proteome is partially derived through the muscle-specific transcription of a subset of genes. Surprisingly, RNA sequencing technologies have also revealed a significant role for muscle-specific alternative splicing in generating protein isoforms that give specialized function to the muscle proteome. In this review, we discuss the current knowledge with respect to the mechanisms that allow pre-mRNA transcripts to undergo muscle-specific alternative splicing while identifying some of the key trans-acting splicing factors essential to the process. The importance of specific splicing events to specialized muscle function is presented along with examples in which dysregulated splicing contributes to myopathies. Though there is now an appreciation that alternative splicing is a major contributor to proteome diversification, the emergence of improved "targeted" proteomic methodologies for detection of specific protein isoforms will soon allow us to better appreciate the extent to which alternative splicing modifies the activity of proteins (and their ability to interact with other proteins) in the skeletal muscle. In addition, we highlight a continued need to better explore the signaling pathways that contribute to the temporal control of trans-acting splicing factor activity to ensure specific protein isoforms are expressed in the proper cellular context. An understanding of the signal-dependent and signal-independent events driving muscle-specific alternative splicing has the potential to provide us with novel therapeutic strategies to treat different myopathies.

  12. Driving a Bus in India: A Study of Indore Bus Rapid Transport System

    Directory of Open Access Journals (Sweden)

    Siddhartha K. RASTOGI

    2017-12-01

    Full Text Available Indore is a typical tier-2 town in the central province of India, fast becoming a regional commercial hub in an economically booming state. The city has a population of over 2 million with no significant public transport. Among the multiple options available, the city started implementing a dedicated corridor based Bus Rapid Transport System (BRTS. The project was jointly mooted by the central, state, and city authorities. However, the system encountered a range of problems, including conflicts and pulls between multiple implementing agencies, delays in delivery of hardware and constructions, land acquisition and compensation, vested interests, public interest litigations, and court orders. The paper analyses the BRTS, introduced in Indore in the midst of 2013, from multiple party perspectives with an underlying search for public policy and public management insights. The broad results indicate feasibility and desirability of the project; however, real problems emerge in public and public perception management.

  13. Optimal Locations of Bus Stops Connecting Subways near Urban Intersections

    Directory of Open Access Journals (Sweden)

    Yuan Cui

    2015-01-01

    Full Text Available Unsuitable locations of bus stops which provide feeder transportation connecting subways near urban intersections usually lead to the low efficiency of public transport and level of passenger service. A multiobjective optimization model to distribute such stop locations is proposed to attain the shortest total walk distance of passengers and minimum delay time of cars through intersections and travel time of buses. The Pareto frontier and optimal solutions for the proposed model are given by the distance-based and enumerative methods. The Xizhimen bus stop is selected to implement case studies for verifying the validity of the proposed model. The analysis of sensitivity on possible solutions is also carried out in the case studies. The results show that the proposed model is capable of optimizing the locations of bus stops connecting subways near intersections and helpful to improve the level of passengers service and operational efficiency of public transportation.

  14. American Fuel Cell Bus Project Evaluation. Second Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-09-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB was developed as part of the Federal Transit Administration's (FTA's) National Fuel Cell Bus Program. Through the non-profit consortia CALSTART, a team led by SunLine Transit Agency and BAE Systems developed a new fuel cell electric bus for demonstration. SunLine added two more AFCBs to its fleet in 2014 and another in 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report summarizes the performance results for the buses through June 2015.

  15. Rethinking passive transport: bus fare exemptions and young people's wellbeing.

    Science.gov (United States)

    Jones, Alasdair; Steinbach, Rebecca; Roberts, Helen; Goodman, Anna; Green, Judith

    2012-05-01

    Much recent public health research has emphasised the health impacts for young people of 'active travel' modes, typically defined as walking and cycling. Less research has focused on public transport modes. Drawing on qualitative data, we examine the links between bus travel and wellbeing in London, where young people currently have free bus travel. Our findings indicate that bus travel can be both a physically and socially active experience for young people. We suggest a more nuanced understanding of 'active travel' is now needed, alongside greater attention to urban public transport networks as key sites that impact on important determinants of wellbeing such as independent mobility and social inclusion. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. RS-485 Bus Design of a Missile Simulation Training System

    Directory of Open Access Journals (Sweden)

    Liu Fang

    2013-07-01

    Full Text Available In a missile simulation training system with one-master and multi-slaves distributed system structure, a universal controller is necessary due to the system composed with several controllers. In this research, the designed controllers communicate with each other and upper control computer through RS-485 field bus. RS-485 bus including interface circuits, transmission protocol, Cyclic Redundancy Check (CRC method and upper control test software is designed and proposed. The universal controller adopting the designed RS-485 interface circuits is connected through twisted-pair and makes the simulation system, then the controller is tested in line. The results show that the RS-485 bus communicates effectively using the protocol and CRC method, data transmission rates reaches 115.2 kbps, and has a good stability.

  17. DESIGN OF CITY BUS LIFT FOR THE HANDICAPPEDS

    Directory of Open Access Journals (Sweden)

    K. Turgut GÜRSEL

    2003-03-01

    Full Text Available Although the technological developments, the handicappeds do not take part sufficiently in the social and economic life, because buildings, road construction features and means of the transport are not suitable for them. In this study, a lift for handicappeds was designed, that is supposed to be installed to the middle door of city buses. The lift, whose installation should require a few changes at the bus, is driven by a hydraulic system from the street to the level of the bus floor and vice-versa. In the work, at first a construction was developed in accordance with the dimensions of the middle door of a city bus. After determining of dimensions of all elements, a hydraulic mechanism was constructed, that raises the handicapped, its wheelchair and the platform. Furthermore the construction of the elements of the system were determined, and its proofs of strength analysis were indicated

  18. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    , and regenerative braking for battery charging. It uses a 19.3 kW Ballard PEM fuel cell, will store 12.6 kg of hydrogen at 350 Bar, and includes a 60 kWh battery storage system. The objectives of the project included the following: (a) To advance commercialization of hydrogen-powered transit buses and supporting infrastructure; (b) To provide public outreach and education by showcasing the operation of a 22-foot fuel cell hybrid shuttle bus and Texas first hydrogen fueling infrastructure; and (c) To showcase operation of zero-emissions vehicle for potential transit applications. As mentioned above, the project successfully demonstrated an early vehicle technology, the Ebus plug-in hybrid fuel cell bus, and that success has led to the acquisition of a more advanced vehicle that can take advantage of the same fueling infrastructure. Needed hydrogen station improvements have been identified that will enhance the capabilities of the fueling infrastructure to serve the new bus and to meet the transit agency needs. Over the course of this project, public officials, local government staff, and transit operators were engaged in outreach and education activities that acquainted them with the real world operation of a fuel cell bus and fueling infrastructure. Transit staff members in the Dallas/Ft. Worth region were invited to a workshop in Arlington, Texas at the North Central Texas Council of Governments to participate in a workshop on hydrogen and fuel cells, and to see the fuel cell bus in operation. The bus was trucked to the meeting for this purpose so that participants could see and ride the bus. Austin area transit staff members visited the fueling site in Austin to be briefed on the bus and to participate in a fueling demonstration. This led to further meetings to determine how a fuel cell bus and fueling station could be deployed at Capital Metro Transit. Target urban regions that expressed additional interest during the project in response to the outreach meetings and

  19. Spatial analysis of bus transport networks using network theory

    Science.gov (United States)

    Shanmukhappa, Tanuja; Ho, Ivan Wang-Hei; Tse, Chi Kong

    2018-07-01

    In this paper, we analyze the bus transport network (BTN) structure considering the spatial embedding of the network for three cities, namely, Hong Kong (HK), London (LD), and Bengaluru (BL). We propose a novel approach called supernode graph structuring for modeling the bus transport network. A static demand estimation procedure is proposed to assign the node weights by considering the points of interests (POIs) and the population distribution in the city over various localized zones. In addition, the end-to-end delay is proposed as a parameter to measure the topological efficiency of the bus networks instead of the shortest distance measure used in previous works. With the aid of supernode graph representation, important network parameters are analyzed for the directed, weighted and geo-referenced bus transport networks. It is observed that the supernode concept has significant advantage in analyzing the inherent topological behavior. For instance, the scale-free and small-world behavior becomes evident with supernode representation as compared to conventional or regular graph representation for the Hong Kong network. Significant improvement in clustering, reduction in path length, and increase in centrality values are observed in all the three networks with supernode representation. The correlation between topologically central nodes and the geographically central nodes reveals the interesting fact that the proposed static demand estimation method for assigning node weights aids in better identifying the geographically significant nodes in the network. The impact of these geographically significant nodes on the local traffic behavior is demonstrated by simulation using the SUMO (Simulation of Urban Mobility) tool which is also supported by real-world empirical data, and our results indicate that the traffic speed around a particular bus stop can reach a jammed state from a free flow state due to the presence of these geographically important nodes. A comparison

  20. Jackson Bar Training Structure Study

    Science.gov (United States)

    2015-05-01

    comparison of the one-dimensional bridge hydraulic routines from: HEC - RAS , HEC -2, and WSPRO. Davis, CA: U.S. Army Corps of Engineers, Hydrologic Engineering...ER D C/ CH L TR -1 5- 4 Jackson Bar Training Structure Study Co as ta l a nd H yd ra ul ic s La bo ra to ry Jeremy A. Sharp and...Leroy Gage), a previously constructed HEC -2 model, and a previously constructed WES physical model from 1987. Three alternatives were modeled in an

  1. The bridge technique for pectus bar fixation: a method to make the bar un-rotatable.

    Science.gov (United States)

    Park, Hyung Joo; Kim, Kyung Soo; Moon, Young Kyu; Lee, Sungsoo

    2015-08-01

    Pectus bar rotation is a major challenge in pectus repair. However, to date, no satisfactory technique to completely eliminate bar displacement has been introduced. Here, we propose a bar fixation technique using a bridge that makes the bar unmovable. The purpose of this study was to determine the efficacy of this bridge technique. A total of 80 patients underwent pectus bar repair of pectus excavatum with the bridge technique from July 2013 to July 2014. The technique involved connecting 2 parallel bars using plate-screws at the ends of the bars. To determine bar position change, the angles between the sternum and pectus bars were measured on postoperative day 5 (POD5) and 4 months (POM4) and compared. The mean patient age was 17.5 years (range, 6-38 years). The mean difference between POD5 and POM4 were 0.23° (P=.602) and 0.35° (P=.338) for the upper and lower bars, respectively. Bar position was virtually unchanged during the follow-up, and there was no bar dislocation or reoperation. A "bridge technique" designed to connect 2 parallel bars using plates and screws was demonstrated as a method to avoid pectus bar displacement. This approach was easy to implement without using sutures or invasive devices. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Has the natural gas fueled bus any future?

    International Nuclear Information System (INIS)

    Riikonen, A.

    2001-01-01

    Helsinki City Transport has decided to operate public transport in the center of the city with tramways and gas-fuelled busses. The decision is that there will be about 100 natural gas fueled busses in Helsinki by the year 2003. European exhaust gas emission (NO x and particulates) regulations have tightened strongly during the past few years. The regulations have forced to search for new fuels by the side of development of diesel engines. Alcohols, in spite of favourable fuel properties, are too expensive, so the use of them needs large subsidies for transportation sector. Gaseous fuels, both LPG and natural gas are suitable fuels for Otto cycle-cycle engines. After the previous oil crisis the interest in gas-fuelled engines has steadily decreased, but at present it is increasing again because of the objectives to decrease emissions of heavy vehicles at the level of gasoline-fuelled vehicles, equipped with three-way catalyst. From the point of view of emissions natural gas and LPG are seen as equivalent alternatives. The price of LPG varies on the basis of demand and on the basis of the prices of other oil products. Refuelling of a vehicle and storage of LPG in liquid form in the tank of the vehicle is easier than refuelling and fuel storage of natural gas. Investments to refuelling equipment of LPG are only 20% of those of the natural gas refuelling systems. The problem of natural gas is also the fact that is not easy to carry in the vehicle. Even if natural gas is compressed to pressure of 200 bars, it requires six times larger tanks if the refuelling intervals are the same. Liquefaction of natural gas reduces the volume significantly, but this is complicated and hence expensive. The tank of a vehicle should be vacuum insulated because the temperature of the LNG is about 160 deg C. Tank volume of LPG is only about twice that of diesel oil. Safety of natural gas is high, because it is lighter than the air, nearly a half of the density of the air. Octane ratings

  3. A 5' splice site enhances the recruitment of basal transcription initiation factors in vivo

    DEFF Research Database (Denmark)

    Damgaard, Christian Kroun; Kahns, Søren; Lykke-Andersen, Søren

    2008-01-01

    RNAs, harboring wild-type or various 5′ splice site mutations, we demonstrate a strong positive correlation between splicing efficiency and transcription activity. Interestingly, a 5′ splice site can stimulate transcription even in the absence of splicing. Chromatin immunoprecipitation experiments show enhanced...... a promoter-proximal 5′ splice site via its U1 snRNA interaction can feed back to stimulate transcription initiation by enhancing preinitiation complex assembly....

  4. Lightweight power bus for a baseload nuclear reactor in space

    International Nuclear Information System (INIS)

    Oberly, C.E.; Massie, L.D.; Hoffman, D.J.

    1989-01-01

    Space environmental interactions with the power distribution/power processing subsystem can become a serious problem for power systems rated at 10's to 100's of kilowatts. Utilization of ceramic superconductors at 1000 A/cm/sup 2/, which has already been demonstrated at 77 K in a conductor configuration may eliminate both bus mass and distribution voltage problems in a high power satellite. The analytical results presented here demonstrate that a superconducting coaxial power transmission bus offers significant benefits in reduced distribution voltage and mass

  5. Quantum bus of metal nanoring with surface plasmon polaritons

    International Nuclear Information System (INIS)

    Lin Zhirong; Guo Guoping; Tu Tao; Li Haiou; Zou Changling; Ren Xifeng; Guo Guangcan; Chen Junxue; Lu Yonghua

    2010-01-01

    We develop an architecture for distributed quantum computation using quantum bus of plasmonic circuits and spin qubits in self-assembled quantum dots. Deterministic quantum gates between two distant spin qubits can be reached by using an adiabatic approach in which quantum dots couple with highly detuned plasmon modes in a metallic nanoring. Plasmonic quantum bus offers a robust and scalable platform for quantum optics experiments and the development of on-chip quantum networks composed of various quantum nodes, such as quantum dots, molecules, and nanoparticles.

  6. Overview of Multi-DC-Bus Solutions for DC Microgrids

    DEFF Research Database (Denmark)

    Ricchiuto, D.; Mastromauro, R.A.; Liserre, Marco

    2013-01-01

    DC Microgrids have recently received a lot of attention in the last years due to high penetration of renewable energy sources as well as distributed energy storage systems. In the future DC microgrids could be preferable respect to AC microgrids in terms of redundancy since multi-DC-Bus solutions...... could provide a continuative power supply to the loads. An overview of Multi-DC-Bus solutions is presented in this paper. The performances are compared on the basis of possible DC microgrid configurations, redundancy, different DC voltage levels....

  7. Bus bays inventory using a terrestrial laser scanning system

    Directory of Open Access Journals (Sweden)

    Bobkowska Katarzyna

    2017-01-01

    Full Text Available This article presents the use of laser scanning technology for the assessment of bus bay geo-location. Ground laser scanning is an effective tool for collecting three-dimensional data. Moreover, the analysis of a point cloud dataset can be a source of a lot of information. The authors have outlined an innovative use of data collection and analysis using the TLS regarding information on the flatness of bus bays. The results were finalized in the form of colour three-dimensional maps of deviations and pavement type.

  8. Design of temperature monitoring system based on CAN bus

    Science.gov (United States)

    Zhang, Li

    2017-10-01

    The remote temperature monitoring system based on the Controller Area Network (CAN) bus is designed to collect the multi-node remote temperature. By using the STM32F103 as main controller and multiple DS18B20s as temperature sensors, the system achieves a master-slave node data acquisition and transmission based on the CAN bus protocol. And making use of the serial port communication technology to communicate with the host computer, the system achieves the function of remote temperature storage, historical data show and the temperature waveform display.

  9. SpliceSeq: a resource for analysis and visualization of RNA-Seq data on alternative splicing and its functional impacts.

    Science.gov (United States)

    Ryan, Michael C; Cleland, James; Kim, RyangGuk; Wong, Wing Chung; Weinstein, John N

    2012-09-15

    SpliceSeq is a resource for RNA-Seq data that provides a clear view of alternative splicing and identifies potential functional changes that result from splice variation. It displays intuitive visualizations and prioritized lists of results that highlight splicing events and their biological consequences. SpliceSeq unambiguously aligns reads to gene splice graphs, facilitating accurate analysis of large, complex transcript variants that cannot be adequately represented in other formats. SpliceSeq is freely available at http://bioinformatics.mdanderson.org/main/SpliceSeq:Overview. The application is a Java program that can be launched via a browser or installed locally. Local installation requires MySQL and Bowtie. mryan@insilico.us.com Supplementary data are available at Bioinformatics online.

  10. Conditional Toxin Splicing Using a Split Intein System.

    Science.gov (United States)

    Alford, Spencer C; O'Sullivan, Connor; Howard, Perry L

    2017-01-01

    Protein toxin splicing mediated by split inteins can be used as a strategy for conditional cell ablation. The approach requires artificial fragmentation of a potent protein toxin and tethering each toxin fragment to a split intein fragment. The toxin-intein fragments are, in turn, fused to dimerization domains, such that addition of a dimerizing agent reconstitutes the split intein. These chimeric toxin-intein fusions remain nontoxic until the dimerizer is added, resulting in activation of intein splicing and ligation of toxin fragments to form an active toxin. Considerations for the engineering and implementation of conditional toxin splicing (CTS) systems include: choice of toxin split site, split site (extein) chemistry, and temperature sensitivity. The following method outlines design criteria and implementation notes for CTS using a previously engineered system for splicing a toxin called sarcin, as well as for developing alternative CTS systems.

  11. Research on Splicing Method of Digital Relic Fragment Model

    Science.gov (United States)

    Yan, X.; Hu, Y.; Hou, M.

    2018-04-01

    In the course of archaeological excavation, a large number of pieces of cultural relics were unearthed, and the restoration of these fragments was done manually by traditional arts and crafts experts. In this process, cultural relics experts often try to splice the existing cultural relics, and then use adhesive to stick together the fragments of correct location, which will cause irreversible secondary damage to cultural relics. In order to minimize such damage, the surveyors combine 3D laser scanning with computer technology, and use the method of establishing digital cultural relics fragments model to make virtual splicing of cultural relics. The 3D software on the common market can basically achieve the model translation and rotation, using this two functions can be achieved manually splicing between models, mosaic records after the completion of the specific location of each piece of fragments, so as to effectively reduce the damage to the relics had tried splicing process.

  12. Seismic retrofit of spliced sleeve connections for precast bridge piers.

    Science.gov (United States)

    2017-03-01

    Grouted Splice Sleeve (GSS) connectors are being considered for connecting bridge columns, footings, and pier caps in Accelerated Bridge Construction (ABC). A repair technique for precast reinforced concrete bridge column-to-footing and column-to-pie...

  13. Herboxidiene triggers splicing repression and abiotic stress responses in plants

    KAUST Repository

    Alshareef, Sahar; Ling, Yu; Butt, Haroon; Mariappan, Kiruthiga G.; Benhamed, Moussa; Mahfouz, Magdy M.

    2017-01-01

    Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small

  14. BaBar Data Aquisition

    CERN Document Server

    Scott, I; Grosso, P; Hamilton, R T; Huffer, M E; O'Grady, C; Russell, J J

    1998-01-01

    The BaBar experiment at the Stanford Linear Accelerator Center is designed to perform a search for CP violation by analysing the decays of a very large sample of B and Bbar mesons produced at the high luminosity PEP-11 accelerator. The data acquisition system must cope with a sustained high event rate, while supporting real time feature extraction and data compression with minimal dead time. The BaBar data acquisition system is based around a common VME interface to the electronics read-out of the separate detector subsystems. Data from the front end electronics is read into commercial VME processors via a custom "personality card" and PCI interface. The commercial CPUs run the Tornado operating system to provide a platform for detector subsystem code to perform the necessary data processing. The data are read out via a non-blocking network switch to a farm of commercial UNIX processors. Careful design of the core data acquisition code has enabled us to sustain events rates in excess of 20 kHz while maintaini...

  15. Boric Acid Reclamation System (BARS)

    International Nuclear Information System (INIS)

    Kniazewycz, B.G.; Markind, J.

    1986-03-01

    KLM Technologies' personnel have identified a Boric Acid Reclamation System (BARS) utilizing reverse osmosis and ultrafiltration to produce a recyclable grade of otherwise waste boric acid at PWRs, thus reducing a major source of low-level radwaste. The design of a prototype BARS as a compact volume reduction system was the result of KLM's Phase 1 Program, and based upon a preliminary feasibility program, which assessed the applicability of membrane technology to refurbish and recycle waste boric acid from floor and equipment drain streams. The analysis of the overall program indicated a substantial savings regarding off-site disposal costs. Today's economic scenario indicates that optimization of volume reduction operation procedures could significantly reduce waste management costs, especially where burial penalties have become more severe. As a reaction to the economic burden imposed by final disposal, many nuclear plants are currently modifying their design and operating philosophies concerning liquid radwaste processing systems to meet stricter environmental regulations, and to derive potential economic benefits by reducing the ever-increasing volumes of wastes that are produced. To effect these changes, innovative practices in waste management and more efficient processing technologies are being successfully implemented

  16. Evaluation of Urban Bus Transport Failure in Gujranwala City of Pakistan

    OpenAIRE

    MUHAMMAD WAQAR ASLAM; ZAHARA BATOOL

    2017-01-01

    This research work has evaluated the performance of urban public bus transport service operating in Gujranwala city of Pakistan. The assessment has been made in terms of financial viability, bus condition, operational performance and user perception. Based on the data requirements, various traffic/transport surveys were conducted including Passenger Boarding and Alighting Survey, Bus Count Survey, Bus Inspection Survey, Illegal Operations Survey and Public Transport User Interview Survey in c...

  17. Active Power Filter DC Bus Voltage Piecewise Reaching Law Variable Structure Control

    OpenAIRE

    Liu, Baolian; Ding, Zujun; Zhao, Huanyu; Jin, Defei

    2014-01-01

    The DC bus voltage stability control is one key technology to ensure that Active Power Filter (APF) operates stably. The external disturbances such as power grid and load fluctuation and the system parameters changing may affect the stability of APF DC bus voltage and the normal operation of APF. The mathematical model of DC bus voltage is established according to power balance principle and a DC bus voltage piecewise reaching law variable structure control algorithm is proposed to solve the ...

  18. Kajian Keamanan Pengoperasian Public Transport Tinjauan Terhadap Kekuatan Rangka Body Bus

    OpenAIRE

    Sudiro, B. M

    2010-01-01

    In order to meet the safety and security of the public transportation, design specification of construction body bus or bus frame structures should have an appropriate structural strength and comply with standard design including the safety factor and selection of material besides static and dynamic testing of the part of body bus. It is also realized that a bus carrying passengers have to give secure to the passengers also driver.The fact that a technology concerning with metal works indus...

  19. Developing a viable electric bus service: the Milton Keynes demonstration project

    OpenAIRE

    Miles, John; Potter, Stephen

    2014-01-01

    Buses can be a serious source of city centre air pollution. Electric buses deliver zero emissions but, because of the time required to recharge, more buses are needed for a given timetable than diesel counterparts, so making mainstream electric bus operations prohibitively expensive.\\ud \\ud Early 2014 saw the implementation in Milton Keynes of an electric bus service designed to overcome this problem. An entire bus route has been converted to electric operation with inductive charging at bus ...

  20. The peculiarities of large intron splicing in animals.

    Directory of Open Access Journals (Sweden)

    Samuel Shepard

    Full Text Available In mammals a considerable 92% of genes contain introns, with hundreds and hundreds of these introns reaching the incredible size of over 50,000 nucleotides. These "large introns" must be spliced out of the pre-mRNA in a timely fashion, which involves bringing together distant 5' and 3' acceptor and donor splice sites. In invertebrates, especially Drosophila, it has been shown that larger introns can be spliced efficiently through a process known as recursive splicing-a consecutive splicing from the 5'-end at a series of combined donor-acceptor splice sites called RP-sites. Using a computational analysis of the genomic sequences, we show that vertebrates lack the proper enrichment of RP-sites in their large introns, and, therefore, require some other method to aid splicing. We analyzed over 15,000 non-redundant, large introns from six mammals, 1,600 from chicken and zebrafish, and 560 non-redundant large introns from five invertebrates. Our bioinformatic investigation demonstrates that, unlike the studied invertebrates, the studied vertebrate genomes contain consistently abundant amounts of direct and complementary strand interspersed repetitive elements (mainly SINEs and LINEs that may form stems with each other in large introns. This examination showed that predicted stems are indeed abundant and stable in the large introns of mammals. We hypothesize that such stems with long loops within large introns allow intron splice sites to find each other more quickly by folding the intronic RNA upon itself at smaller intervals and, thus, reducing the distance between donor and acceptor sites.

  1. The Carnegie-Irvine Galaxy Survey. V. Statistical Study of Bars and Buckled Bars

    Science.gov (United States)

    Li, Zhao-Yu; Ho, Luis C.; Barth, Aaron J.

    2017-08-01

    Simulations have shown that bars are subject to a vertical buckling instability that transforms thin bars into boxy or peanut-shaped structures, but the physical conditions necessary for buckling to occur are not fully understood. We use the large sample of local disk galaxies in the Carnegie-Irvine Galaxy Survey to examine the incidence of bars and buckled bars across the Hubble sequence. Depending on the disk inclination angle (I), a buckled bar reveals itself as either a boxy/peanut-shaped bulge (at high I) or as a barlens structure (at low I). We visually identify bars, boxy/peanut-shaped bulges, and barlenses, and examine the dependence of bar and buckled bar fractions on host galaxy properties, including Hubble type, stellar mass, color, and gas mass fraction. We find that the barred and unbarred disks show similar distributions in these physical parameters. The bar fraction is higher (70%-80%) in late-type disks with low stellar mass (M * 1010.5 M ⊙), and decreases with higher gas mass ratio. These results suggest that bars are more difficult to grow in massive disks that are dynamically hotter than low-mass disks. However, once a bar forms, it can easily buckle in the massive disks, where a deeper potential can sustain the vertical resonant orbits. We also find a probable buckling bar candidate (ESO 506-G004) that could provide further clues to understand the timescale of the buckling process.

  2. Extruded bar reinforced structure and manufacturing procedures

    International Nuclear Information System (INIS)

    Truchet, J.M.; Bozetto, P.

    1989-01-01

    A cooling tower has horizontal hoops connected by two inclined sets of bars to form a trellis of equilateral triangle anchored in the ground. The bars and hoops are connected at the corners of the triangle. A skin stretched over the trellis defines the tower. The bars are made with thermosetting resin reinforced by fibres. The fabrication of such tower is cheep and simple it can be used for every type of electrical power station, nuclear or not [fr

  3. DETERMINATION OF THE DELAY DEPARTURE TIME OF THE BUS FROM STOPPING POINT INTO THE CAR FLOW

    Directory of Open Access Journals (Sweden)

    P. Gorbachov

    2014-10-01

    Full Text Available An analytical model for estimation of the bus departure delax from the bus stopping point after passenger loading is presented. The model allows to take into account the traffic density on a particular road section of the transport network, as well as the bus parameters.

  4. Computer-Based Video Instruction to Teach Students with Intellectual Disabilities to Use Public Bus Transportation

    Science.gov (United States)

    Mechling, Linda; O'Brien, Eileen

    2010-01-01

    This study investigated the effectiveness of computer-based video instruction (CBVI) to teach three young adults with moderate intellectual disabilities to push a "request to stop bus signal" and exit a city bus in response to target landmarks. A multiple probe design across three students and one bus route was used to evaluate effectiveness of…

  5. 49 CFR 383.123 - Requirements for a school bus endorsement.

    Science.gov (United States)

    2010-10-01

    ... devices required for school buses by State or Federal law or regulation. (ii) Emergency exits and... 49 Transportation 5 2010-10-01 2010-10-01 false Requirements for a school bus endorsement. 383.123... Requirements for a school bus endorsement. (a) An applicant for a school bus endorsement must satisfy the...

  6. The Role of the Coroner in School Bus Accident Prevention: Some Recommendations.

    Science.gov (United States)

    Fox, Michael

    1995-01-01

    Following the deaths of two elementary school students in bus-related accidents in 1992, the Coroner of Quebec held extensive hearings investigating school bus safety and accident prevention. A subsequent report addressed responsibilities of government and school board officials to correct deficiencies in school bus services and provided…

  7. School Bus Safety: What Can Our Schools Do to Protect Our Children?

    Science.gov (United States)

    Dargan, Thomas J.; Silverstone, Adam H.

    2014-01-01

    School districts and school bus contractors are entrusted with the most important of all road users--our nation's children. In the wake of recent newsworthy accidents and attention grabbing headlines regarding unfit bus drivers, claims premised upon school bus accidents have become increasingly tangential and, in turn, personal injury attorneys…

  8. Splicing regulatory factors, ageing and age-related disease.

    Science.gov (United States)

    Latorre, Eva; Harries, Lorna W

    2017-07-01

    Alternative splicing is a co-transcriptional process, which allows for the production of multiple transcripts from a single gene and is emerging as an important control point for gene expression. Alternatively expressed isoforms often have antagonistic function and differential temporal or spatial expression patterns, yielding enormous plasticity and adaptability to cells and increasing their ability to respond to environmental challenge. The regulation of alternative splicing is critical for numerous cellular functions in both pathological and physiological conditions, and deregulated alternative splicing is a key feature of common chronic diseases. Isoform choice is controlled by a battery of splicing regulatory proteins, which include the serine arginine rich (SRSF) proteins and the heterogeneous ribonucleoprotein (hnRNP) classes of genes. These important splicing regulators have been implicated in age-related disease, and in the ageing process itself. This review will outline the important contribution of splicing regulator proteins to ageing and age-related disease. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Reflections on protein splicing: structures, functions and mechanisms

    Science.gov (United States)

    Anraku, Yasuhiro; Satow, Yoshinori

    2009-01-01

    Twenty years ago, evidence that one gene produces two enzymes via protein splicing emerged from structural and expression studies of the VMA1 gene in Saccharomyces cerevisiae. VMA1 consists of a single open reading frame and contains two independent genetic information for Vma1p (a catalytic 70-kDa subunit of the vacuolar H+-ATPase) and VDE (a 50-kDa DNA endonuclease) as an in-frame spliced insert in the gene. Protein splicing is a posttranslational cellular process, in which an intervening polypeptide termed as the VMA1 intein is self-catalytically excised out from a nascent 120-kDa VMA1 precursor and two flanking polypeptides of the N- and C-exteins are ligated to produce the mature Vma1p. Subsequent studies have demonstrated that protein splicing is not unique to the VMA1 precursor and there are many operons in nature, which implement genetic information editing at protein level. To elucidate its structure-directed chemical mechanisms, a series of biochemical and crystal structural studies has been carried out with the use of various VMA1 recombinants. This article summarizes a VDE-mediated self-catalytic mechanism for protein splicing that is triggered and terminated solely via thiazolidine intermediates with tetrahedral configurations formed within the splicing sites where proton ingress and egress are driven by balanced protonation and deprotonation. PMID:19907126

  10. Cell-Type-Specific Splicing of Piezo2 Regulates Mechanotransduction

    Directory of Open Access Journals (Sweden)

    Marcin Szczot

    2017-12-01

    Full Text Available Summary: Piezo2 is a mechanically activated ion channel required for touch discrimination, vibration detection, and proprioception. Here, we discovered that Piezo2 is extensively spliced, producing different Piezo2 isoforms with distinct properties. Sensory neurons from both mice and humans express a large repertoire of Piezo2 variants, whereas non-neuronal tissues express predominantly a single isoform. Notably, even within sensory ganglia, we demonstrate the splicing of Piezo2 to be cell type specific. Biophysical characterization revealed substantial differences in ion permeability, sensitivity to calcium modulation, and inactivation kinetics among Piezo2 splice variants. Together, our results describe, at the molecular level, a potential mechanism by which transduction is tuned, permitting the detection of a variety of mechanosensory stimuli. : Szczot et al. find that the mechanoreceptor Piezo2 is extensively alternatively spliced, generating multiple distinct isoforms. Their findings indicate that these splice products have specific tissue and cell type expression patterns and exhibit differences in receptor properties. Keywords: Piezo, touch, sensation, ion-channel, splicing

  11. Systematic Analysis of Splice-Site-Creating Mutations in Cancer.

    Science.gov (United States)

    Jayasinghe, Reyka G; Cao, Song; Gao, Qingsong; Wendl, Michael C; Vo, Nam Sy; Reynolds, Sheila M; Zhao, Yanyan; Climente-González, Héctor; Chai, Shengjie; Wang, Fang; Varghese, Rajees; Huang, Mo; Liang, Wen-Wei; Wyczalkowski, Matthew A; Sengupta, Sohini; Li, Zhi; Payne, Samuel H; Fenyö, David; Miner, Jeffrey H; Walter, Matthew J; Vincent, Benjamin; Eyras, Eduardo; Chen, Ken; Shmulevich, Ilya; Chen, Feng; Ding, Li

    2018-04-03

    For the past decade, cancer genomic studies have focused on mutations leading to splice-site disruption, overlooking those having splice-creating potential. Here, we applied a bioinformatic tool, MiSplice, for the large-scale discovery of splice-site-creating mutations (SCMs) across 8,656 TCGA tumors. We report 1,964 originally mis-annotated mutations having clear evidence of creating alternative splice junctions. TP53 and GATA3 have 26 and 18 SCMs, respectively, and ATRX has 5 from lower-grade gliomas. Mutations in 11 genes, including PARP1, BRCA1, and BAP1, were experimentally validated for splice-site-creating function. Notably, we found that neoantigens induced by SCMs are likely several folds more immunogenic compared to missense mutations, exemplified by the recurrent GATA3 SCM. Further, high expression of PD-1 and PD-L1 was observed in tumors with SCMs, suggesting candidates for immune blockade therapy. Our work highlights the importance of integrating DNA and RNA data for understanding the functional and the clinical implications of mutations in human diseases. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  12. The Functional Impact of Alternative Splicing in Cancer.

    Science.gov (United States)

    Climente-González, Héctor; Porta-Pardo, Eduard; Godzik, Adam; Eyras, Eduardo

    2017-08-29

    Alternative splicing changes are frequently observed in cancer and are starting to be recognized as important signatures for tumor progression and therapy. However, their functional impact and relevance to tumorigenesis remain mostly unknown. We carried out a systematic analysis to characterize the potential functional consequences of alternative splicing changes in thousands of tumor samples. This analysis revealed that a subset of alternative splicing changes affect protein domain families that are frequently mutated in tumors and potentially disrupt protein-protein interactions in cancer-related pathways. Moreover, there was a negative correlation between the number of these alternative splicing changes in a sample and the number of somatic mutations in drivers. We propose that a subset of the alternative splicing changes observed in tumors may represent independent oncogenic processes that could be relevant to explain the functional transformations in cancer, and some of them could potentially be considered alternative splicing drivers (AS drivers). Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  13. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    Directory of Open Access Journals (Sweden)

    Zodwa Dlamini

    2015-11-01

    Full Text Available Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 family have alternatively spliced isoforms that lack important active domains. These isoforms can play a negative regulatory role by binding to and inhibiting the pro-apoptotic forms. Alternative splicing is observed to be increased in various cardiovascular diseases with the level of alternate transcripts increasing elevated in diseased hearts compared to healthy subjects. In many cases these isoforms appear to be the underlying cause of the disease, while in others they may be induced in response to cardiovascular pathologies. Regardless of this, the detection of alternate splicing events in the heart can serve as useful diagnostic or prognostic tools, while those splicing events that seem to play a causative role in cardiovascular disease make attractive future drug targets.

  14. TESTING THEORIES IN BARRED-SPIRAL GALAXIES

    International Nuclear Information System (INIS)

    Martínez-García, Eric E.

    2012-01-01

    According to one version of the recently proposed 'manifold' theory that explains the origin of spirals and rings in relation to chaotic orbits, galaxies with stronger bars should have a higher spiral arms pitch angle when compared to galaxies with weaker bars. A subsample of barred-spiral galaxies in the Ohio State University Bright Galaxy Survey was used to analyze the spiral arms pitch angle. These were compared with bar strengths taken from the literature. It was found that the galaxies in which the spiral arms maintain a logarithmic shape for more than 70° seem to corroborate the predicted trend.

  15. Nuss bar migrations: occurrence and classification

    International Nuclear Information System (INIS)

    Binkovitz, Lauren E.; Binkovitz, Larry A.; Zendejas, Benjamin; Moir, Christopher R.

    2016-01-01

    Pectus excavatum results from dorsal deviation of the sternum causing narrowing of the anterior-posterior diameter of the chest. It can result in significant cosmetic deformities and cardiopulmonary compromise if severe. The Nuss procedure is a minimally invasive technique that involves placing a thin horizontally oriented metal bar below the dorsal sternal apex for correction of the pectus deformity. To identify the frequency and types of Nuss bar migrations, to present a new categorization of bar migrations, and to present examples of true migrations and pseudomigrations. We retrospectively reviewed the electronic medical records and all pertinent radiologic studies of 311 pediatric patients who underwent a Nuss procedure. We evaluated the frequency and type of bar migrations. Bar migration was demonstrated in 23 of 311 patients (7%) and occurred within a mean period of 26 days after surgery. Bar migrations were subjectively defined as deviation of the bar from the position demonstrated on the immediate postoperative radiographs and categorized as superior, inferior, rotation, lateral or flipped using a new classification system. Sixteen of the 23 migrations required re-operation. Nuss bar migration can be diagnosed with careful evaluation of serial radiographs. Nuss bar migration has a wide variety of appearances and requires exclusion of pseudomigration resulting from changes in patient positioning between radiologic examinations. (orig.)

  16. Nuss bar migrations: occurrence and classification

    Energy Technology Data Exchange (ETDEWEB)

    Binkovitz, Lauren E.; Binkovitz, Larry A. [Mayo Clinic, Department of Radiology, Rochester, MN (United States); Zendejas, Benjamin; Moir, Christopher R. [Mayo Clinic, Department of Surgery, Rochester, MN (United States)

    2016-12-15

    Pectus excavatum results from dorsal deviation of the sternum causing narrowing of the anterior-posterior diameter of the chest. It can result in significant cosmetic deformities and cardiopulmonary compromise if severe. The Nuss procedure is a minimally invasive technique that involves placing a thin horizontally oriented metal bar below the dorsal sternal apex for correction of the pectus deformity. To identify the frequency and types of Nuss bar migrations, to present a new categorization of bar migrations, and to present examples of true migrations and pseudomigrations. We retrospectively reviewed the electronic medical records and all pertinent radiologic studies of 311 pediatric patients who underwent a Nuss procedure. We evaluated the frequency and type of bar migrations. Bar migration was demonstrated in 23 of 311 patients (7%) and occurred within a mean period of 26 days after surgery. Bar migrations were subjectively defined as deviation of the bar from the position demonstrated on the immediate postoperative radiographs and categorized as superior, inferior, rotation, lateral or flipped using a new classification system. Sixteen of the 23 migrations required re-operation. Nuss bar migration can be diagnosed with careful evaluation of serial radiographs. Nuss bar migration has a wide variety of appearances and requires exclusion of pseudomigration resulting from changes in patient positioning between radiologic examinations. (orig.)

  17. Old-Fashioned Bus Trips: New Age Professional Development

    Science.gov (United States)

    Feldhues, Katherine; Epley, Hannah K.

    2018-01-01

    Two 4-H Camp-related bus tours offered new nontraditional professional development (PD) experiences that better align Extension's PD opportunities with the organization's experiential education pedagogy. Creating quality PD opportunities for employees is important because such experiences can affect overall work performance, community connections,…

  18. Analyzing injury severity of bus passengers with different movements.

    Science.gov (United States)

    Li, Duo; Zhao, Yifei; Bai, Qiang; Zhou, Bei; Ling, Hongbiao

    2017-07-04

    Though public transport vehicles are rarely involved in mass casualty accidents, when they are, the number of injuries and fatalities is usually high due to the high passenger capacity. Of the few studies that have been conducted on bus safety, the majority focused on vehicle safety features, road environmental factors, as well as driver characteristics. Nevertheless, few studies have attempted to investigate the underlying risk factors related to bus occupants. This article presents an investigation aimed at identifying the risk factors affecting injury severity of bus passengers with different movements. Three different passenger movement types including standing, seated, and boarding/alighting were analyzed individually using classification and regression tree (CART) method based on publicly available accident database of Great Britain. According to the results of exploratory analyses, passenger age and vehicle maneuver are associated with passenger injury severity in all 3 types of accidents. Moreover, the variable "skidding and overturning" is associated with injury severity of seated passengers and driver age is correlated with injury severity of standing and boarding/alighting passengers. The CART method shows its ability to identify and easily explain the complicated patterns affecting passenger injury severity. Several countermeasures to reduce bus passenger injury severity are recommended.

  19. 49 CFR 374.317 - Identification-bus and driver.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Identification-bus and driver. 374.317 Section 374... SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Adequacy of Intercity Motor Common Carrier Passenger Service § 374.317 Identification...

  20. A Quantitative Economics Of Seasonality In Bus Patronage In Nigeria

    African Journals Online (AJOL)

    Seasonal variation is a well-known phenomenon, in many endeavrous and enterprises. Passenger of patronage of road transportation by bus is generally implicated as exhibiting excessive seasonality. The study thus set out to empirically investigate the myth or reality of the thesis that trip generation and trip attraction can ...

  1. Aerodynamic study of state transport bus using computational fluid dynamics

    Science.gov (United States)

    Kanekar, Siddhesh; Thakre, Prashant; Rajkumar, E.

    2017-11-01

    The main purpose of this study was to develop the aerodynamic study of a Maharashtra state road transport bus. The rising fuel price and strict government regulations makes the road transport uneconomical now days. With the objective of increasing fuel efficiency and reducing the emission of harmful exhaust gases. It has been proven experimentally that vehicle consumes almost 40% of the available useful engine power to overcome the drag resistance. This provides us a huge scope to study the influence of aerodynamic drag. The initial of the project was to identify the drag coefficient of the existing ordinary type model called “Parivartan” from ANSYS fluent. After preliminary analysis of the existing model corresponding changes are made in such a way that their implementation should be possible at workshop level. The simulation of the air flow over the bus was performed in two steps: design on SolidWorks CAD and ANSYS (FLUENT) is used as a virtual analysis tool to estimate the drag coefficient of the bus. We have used the turbulence models k-ε Realizable having a better approximation of the actual result. Around 28% improvement in the drag coefficient is achieved by CFD driven changes in the bus design. Coefficient of drag is improved by 28% and fuel efficiency increased by 20% by CFD driven changes.

  2. Staying Safe in the Car and on the Bus

    Science.gov (United States)

    ... a booster! Get in the Back Here's another important safety rule: Sit in the back seat. Kids 12 ... or band concerts once in a while, it's important to follow these rules. When you see the bus driving up, everyone waiting should get into a line. ...

  3. Bus-oriented digital control techniques in nuclear power plants

    International Nuclear Information System (INIS)

    Salm, M.

    1987-01-01

    The author states the conservative principles which govern the authorization procedures for nuclear reactor control systems. Using the example of a feedwater supply regulator, employing a digital, bus-oriented control system, he describes how the stigma attached to the word nuclear can be alleviated. (G.T.H.)

  4. Energy efficient motion control of the electric bus on route

    Science.gov (United States)

    Kotiev, G. O.; Butarovich, D. O.; Kositsyn, B. B.

    2018-02-01

    At present, the urgent problem is the reduction of energy costs of urban motor transport. The article proposes a method of solving this problem by developing an energy-efficient law governing the movement of an electric bus along a city route. To solve this problem, an algorithm is developed based on the dynamic programming method. The proposed method allows you to take into account the constraints imposed on the phase coordinates, control action, as well as on the time of the route. In the course of solving the problem, the model of rectilinear motion of an electric bus on a horizontal reference surface is considered, taking into account the assumptions that allow it to be adapted for the implementation of the method. For the formation of a control action in the equations of motion dynamics, an algorithm for changing the traction / braking torque on the wheels of an electric bus is considered, depending on the magnitude of the control parameter and the speed of motion. An optimal phase trajectory was obtained on a selected section of the road for the prototype of an electric bus. The article presents the comparison of simulation results obtained with the optimal energy efficient control law with the results obtained by a test driver. The comparison proved feasibility of the energy efficient control law for the automobile city electric transport.

  5. Economic impacts of bus rapid transit in southeast Michigan.

    Science.gov (United States)

    2015-12-01

    In recent years, Bus Rapid Transit (BRT) has generated great interest across the United States. There are more than 20 BRT : systems in existence, and more are in the planning stage (including in Detroit). Within the next few years, BRT will be plann...

  6. Improving School Bus Safety. Transportation Research Board Special Report 222.

    Science.gov (United States)

    National Academy of Sciences - National Research Council, Washington, DC. Transportation Research Board.

    While school buses transport more passengers per trip, the rate of occupant fatalities per mile driven for school buses is one-quarter that for passenger cars. Nevertheless, the public expects school districts and other school bus operators to take all reasonable precautions to protect children as they travel to and from school. Although a variety…

  7. Implementation of a Walking School Bus: Lessons Learned

    Science.gov (United States)

    Kong, Alberta S.; Sussman, Andrew L.; Negrete, Sylvia; Patterson, Nissa; Mittleman, Rachel; Hough, Richard

    2009-01-01

    Background: Obesity is rapidly becoming the most common chronic medical condition affecting children. To address this crisis, an Albuquerque, New Mexico, elementary school partnered with University of New Mexico researchers and conducted a Walking School Bus (WSB). The purpose of this article is to examine the feasibility of implementing a WSB.…

  8. Optical bus of centralized relay protection and automation system of ...

    African Journals Online (AJOL)

    The article deals with the system of information collection and transfer from a centralized relay protection and automation system for medium voltage electrical units based on a passive optical bus. The issues of electromagnetic compatibility of technical devices are also considered, and the intensity of electromagnetic ...

  9. A Novel Time Synchronization Method for Dynamic Reconfigurable Bus

    Directory of Open Access Journals (Sweden)

    Zhang Weigong

    2016-01-01

    Full Text Available UM-BUS is a novel dynamically reconfigurable high-speed serial bus for embedded systems. It can achieve fault tolerance by detecting the channel status in real time and reconfigure dynamically at run-time. The bus supports direct interconnections between up to eight master nodes and multiple slave nodes. In order to solve the time synchronization problem among master nodes, this paper proposes a novel time synchronization method, which can meet the requirement of time precision in UM-BUS. In this proposed method, time is firstly broadcasted through time broadcast packets. Then, the transmission delay and time deviations via three handshakes during link self-checking and channel detection can be worked out referring to the IEEE 1588 protocol. Thereby, each node calibrates its own time according to the broadcasted time. The proposed method has been proved to meet the requirement of real-time time synchronization. The experimental results show that the synchronous precision can achieve a bias less than 20 ns.

  10. Bussikontsern Go Bus ihaldab Harjumaa turgu / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2005-01-01

    AS-i Tarbus ja kaheksa bussiettevõtte ühinemisel moodustati AS Go Bus, ettevõte plaanib veel mitme bussifirma liitmist. Atko Grupi juhatuse esimehe Margo Tomingase, AS Sebe nõukogu esimehe Hugo Osula arvamus. Lisa: Konkurentsiamet hoiab silma peal

  11. Spanish version of Bus Drivers' Job Demands Scale (BDJD-24).

    Science.gov (United States)

    Boada-Grau, Joan; Prizmic-Kuzmica, Aldo-Javier; González-Fernández, Marcos-David; Vigil-Colet, Andreu

    2013-01-01

    Karasek and Theorell's Job Demands-Control Model argues that adverse health-related outcomes, both psychological and physiological, arise from a combination of high job demand and a low level of job control. The objective was to adapt Meijman and Kompier's Bus Drivers' Job Demands Scale (BDJD-24), which enables us to assess the job demands of bus drivers, to Spanish. The final version of the Spanish adaptation was applied to a sample made up of 287 bus drivers living in Spain (80.1% men and 19.9% women), whose average age was 40.44 (SD= 11.78). The results yielded a three-factor structure for the scale used: Time Pressure, Safety, and Passengers. These findings confirm that the Spanish version replicates the factor structure of the original English scale. The reliability of the three subscales was acceptable, ranging from .75 to .84. Furthermore, the subscales were also related to different external correlates and to other scales and showed good convergent and criterion validity. The present instrument can be used to evaluate job demands of bus drivers, as its psychometrics are substantially sound.

  12. High-Tech School Bus Teaches Students on the Road

    Science.gov (United States)

    Katims, Lauren

    2011-01-01

    Last year, kindergarten through high school students in the rural Hector, Arkansas, School District barely had the technology resources that keep kids interested in math and science. This year, they potentially have the most advanced resources in the country--before they even step into the classroom. One school bus in Arkansas' Pope County has…

  13. Measuring Bus Drivers' Occupational Stress Under Changing Working Conditions

    NARCIS (Netherlands)

    Hlotova, Y.; Cats, O.; Meijer, S.A.

    2014-01-01

    Stress is an immense problem in modern society; approximately half of all occupational illnesses are directly or indirectly related to stress. The work of a bus driver is typically associated with high stress levels that negatively influence individual well-being as well as workforce management. The

  14. Steering characteristic of an articulated bus under quasi steady maneuvering

    Science.gov (United States)

    Ubaidillah, Setiawan, Budi Agus; Aridharma, Airlangga Putra; Lenggana, Bhre Wangsa; Caesar, Bernardus Placenta Previo

    2018-02-01

    Articulated buses have been being preferred as public transportation modes due to their operational capacity. Therefore, passenger safety must be the priority of this public service vehicle. This research focused on the analytical approach of steering characteristics of an articulated bus when it maneuvered steadily. Such turning condition could be referred as a stability parameter of the bus for preliminary handling assessment. The analytical approach employed kinematics relationship between front and rear bodies as well as steering capabilities. The quasi steady model was developed to determine steering parameters such as turning radius, oversteer, and understeer. The mathematical model was useful for determining both coefficients of understeer and oversteer. The dimension of articulated bus followed a commonly used bus as utilized in Trans Jakarta busses. Based on the simulation, for one minimum center of the body, the turning radius was calculated about 8.8 m and 7.6 m at steady turning speed of 10 km/h. In neutral condition, the minimum road radius should be 6.5 m at 10 km/h and 6.9 m at 40 km/h. For two centers of the body and oversteer condition, the front body has the turning radius of 8.8 m, while, the rear body has the turning radius of 9.8 m at both turning speeds of 40 km/h. The other steering parameters were discussed accordingly.

  15. The Spliced Leader Trans-Splicing Mechanism in Different Organisms: Molecular Details and Possible Biological Roles

    Directory of Open Access Journals (Sweden)

    Mainá eBitar

    2013-10-01

    Full Text Available The spliced leader (SL is a gene that generates a functional ncRNA that is composed of two regions: an intronic region of unknown function (SLi and an exonic region (SLe, which is transferred to the 5’ end of independent transcripts yielding mature mRNAs, in a process known as spliced leader trans-splicing (SLTS. The best described function for SLTS is to solve polycistronic transcripts into monocistronic units, specifically in Trypanosomatids. In other metazoans, it is speculated that the SLe addition could lead to increased mRNA stability, differential recruitment of the translational machinery, modification of the 5' region or a combination of these effects. Although important aspects of this mechanism have been revealed, several features remain to be elucidated. We have analyzed 157 SLe sequences from 148 species from 7 phyla and found a high degree of conservation among the sequences of species from the same phylum, although no considerable similarity seems to exist between sequences of species from different phyla. When analyzing case studies, we found evidence that a given SLe will always be related to a given set of transcripts in different species from the same phylum, and therefore, different SLe sequences from the same species would regulate different sets of transcripts. In addition, we have observed distinct transcript categories to be preferential targets for the SLe addition in different phyla. This work sheds light into crucial and controversial aspects of the SLTS mechanism. It represents a comprehensive study concerning various species and different characteristics of this important post-transcriptional regulatory mechanism.

  16. Performance simulation of BaBar DIRC bar boxes in TORCH

    Science.gov (United States)

    Föhl, K.; Brook, N.; Castillo García, L.; Cussans, D.; Forty, R.; Frei, C.; Gao, R.; Gys, T.; Harnew, N.; Piedigrossi, D.; Rademacker, J.; Ros García, A.; van Dijk, M.

    2017-12-01

    TORCH is a large-area precision time-of-flight detector based on the DIRC principle. The DIRC bar boxes of the BaBar experiment at SLAC could possibly be reused to form a part of the TORCH detector time-of-flight wall area, proposed to provide positive particle identification of low momentum kaons in the LHCb experiment at CERN. For a potential integration of BaBar bar boxes into TORCH, new imaging readout optics are required. From the several designs of readout optics that have been considered, two are used in this paper to study the effect of BaBar bar optical imperfections on the detector reconstruction performance. The kaon-pion separation powers obtained from analysing simulated photon hit patterns show the performance reduction for a BaBar bar of non-square geometry compared to a perfectly rectangular cross section.

  17. submitter Performance simulation of BaBar DIRC bar boxes in TORCH

    CERN Document Server

    Föhl, K; Castillo García, L; Cussans, D; Forty, R; Frei, C; Gao, R; Gys, T; Harnew, N; Piedigrossi, D; Rademacker, J; Ros García, A; van Dijk, M

    2017-01-01

    TORCH is a large-area precision time-of-flight detector based on the DIRC principle. The DIRC bar boxes of the BaBar experiment at SLAC could possibly be reused to form a part of the TORCH detector time-of-flight wall area, proposed to provide positive particle identification of low momentum kaons in the LHCb experiment at CERN. For a potential integration of BaBar bar boxes into TORCH, new imaging readout optics are required. From the several designs of readout optics that have been considered, two are used in this paper to study the effect of BaBar bar optical imperfections on the detector reconstruction performance. The kaon-pion separation powers obtained from analysing simulated photon hit patterns show the performance reduction for a BaBar bar of non-square geometry compared to a perfectly rectangular cross section.

  18. GeoBus: sharing science research with schools

    Science.gov (United States)

    Roper, Kathryn; Robinson, Ruth; Moorhouse, Ben

    2016-04-01

    GeoBus (www.geobus.org.uk) is an educational outreach project that was developed in 2012 by the Department of Earth and Environmental Sciences at the University of St Andrews, and it is currently sponsored by industry, NERC, The Crown Estate, and the Scottish Government. The aims of GeoBus are to support the teaching of Earth Science in secondary (middle and high) schools by providing teaching support to schools that have little or no experience in teaching this subject. This is, in part, done through the sharing of new science research outcomes and the experiences of young researchers with school pupils to provide a bridge between industry, higher education institutions, research councils and schools. Since its launch, over 40,000 pupils will have been involved in experiential Earth science learning activities in 190 different schools (over 400 separate visits) across the length and breadth of Scotland: many of these schools are in remote and disadvantaged regions. A new GeoBus project is under development within the Department of Earth Sciences at UCL in London. A key aim of GeoBus is to incorporate new research into our workshops with the main challenge being the development of appropriate resources that incorporate the key learning aims and requirements of the science and geography curricula. GeoBus works closely with researchers, teachers and educational practitioners to tailor the research outcomes to the curricula as much as possible. Over the past four years, GeoBus has developed 17 workshops, 5 challenge events and extensive field trips and each of these activities are trialled and evaluated within the university, and adjustments are made before the activities are delivered in schools. Activities are continually reviewed and further developments are made in response to both teacher and pupil feedback. This critical reflection of the project's success and impact is important to insure a positive and significant contribution to the science learning in

  19. Real life testing of a Hybrid PEM Fuel Cell Bus

    Science.gov (United States)

    Folkesson, Anders; Andersson, Christian; Alvfors, Per; Alaküla, Mats; Overgaard, Lars

    Fuel cells produce low quantities of local emissions, if any, and are therefore one of the most promising alternatives to internal combustion engines as the main power source in future vehicles. It is likely that urban buses will be among the first commercial applications for fuel cells in vehicles. This is due to the fact that urban buses are highly visible for the public, they contribute significantly to air pollution in urban areas, they have small limitations in weight and volume and fuelling is handled via a centralised infrastructure. Results and experiences from real life measurements of energy flows in a Scania Hybrid PEM Fuel Cell Concept Bus are presented in this paper. The tests consist of measurements during several standard duty cycles. The efficiency of the fuel cell system and of the complete vehicle are presented and discussed. The net efficiency of the fuel cell system was approximately 40% and the fuel consumption of the concept bus is between 42 and 48% lower compared to a standard Scania bus. Energy recovery by regenerative braking saves up 28% energy. Bus subsystems such as the pneumatic system for door opening, suspension and brakes, the hydraulic power steering, the 24 V grid, the water pump and the cooling fans consume approximately 7% of the energy in the fuel input or 17% of the net power output from the fuel cell system. The bus was built by a number of companies in a project partly financed by the European Commission's Joule programme. The comprehensive testing is partly financed by the Swedish programme "Den Gröna Bilen" (The Green Car). A 50 kW el fuel cell system is the power source and a high voltage battery pack works as an energy buffer and power booster. The fuel, compressed hydrogen, is stored in two high-pressure stainless steel vessels mounted on the roof of the bus. The bus has a series hybrid electric driveline with wheel hub motors with a maximum power of 100 kW. Hybrid Fuel Cell Buses have a big potential, but there are

  20. Characterisation of an urban bus network for environmental purposes.

    Science.gov (United States)

    André, Michel; Villanova, André

    2004-12-01

    Since pollutant emissions are closely related to the operating conditions of vehicles, their evaluation usually involves studying these operating conditions (through bus instrumentation and monitoring under actual operation), the design of representative driving or engine test cycles and the measurement of pollutant emissions. A preliminary characterisation of the routes on a bus network should make it possible to identify typical routes, the driving conditions and pollutant emissions of which are then studied. Two approaches are envisaged and applied to the Paris area, for which a wealth of information is available, which should be transferable to other bus networks. Both approaches are based on factorial analysis and automatic clustering, to allow optimum description and the identification of a pertinent typology of the bus routes in several classes. The first attempt at characterisation is based on statistics relating to bus operations: route characteristics (length, dedicated bus lanes, number of stops, location of stops: schools, tourist sites, hospitals, railways or underground stations), travel time, commercial speed, annual statistics (number of passengers, number of vehicles per hour, total kilometres), the irregularity of travel (variation of travel times, injuries, congestion.), as well as information on the problems encountered (congestion, distribution of the passenger load, junctions, bends). A second approach is based on the analysis of the "urban context" in which buses are driven. Population, employment, housing, road network, traffic and places that generate or disturb traffic (schools, railway stations, shopping areas, etc.) are calculated for the Ile de France region, by cells of 100 x 100 m, and collected in a geographical information system (GIS). Statistical analyses enable a typology of these urban cells to be established, the main parameters being density, type of housing, road types and traffic levels. The bus routes are then analysed

  1. THE PREVALENCE OF MUSCULOSKELETAL DISORDERS AMONG BUS DRIVERS IN TRICITY

    Directory of Open Access Journals (Sweden)

    Lalit

    2015-10-01

    Full Text Available Background: Musculoskeletal disorders are widespread in many countries around the world. It has been reported that about 58 percent of the world's population over the age of 10 years spent one third of their life span at work. The population at a high risk include nursing facilities, transportation, mining, food processing, leather tanning, heavy and light manufacturing. Transport workers have been found to be at high risk of developing work related musculoskeletal disorders (WRMSDs. There has been literature evidence regarding the prevalence of musculoskeletal disorders in bus drivers of various cities of different countries. But no study has been done so far in Tricity (Chandigarh, Panchkula and Mohali for the same. The purpose of this study is to investigate the prevalence and characteristics of work related musculoskeletal disorders (WRMSDs among bus drivers of Tricity. Methods: 300 bus drivers were included in the study according to the inclusion and exclusion criteria. The standardized Nordic questionnaire for musculoskeletal disorder and a self administered questionnaire were filled by therapist after the personal interview of each driver. Results: Unpaired t test was used to measure the difference in variable of two groups and Karl Pearson’s correlation coefficient was used to determine the correlation between two entities. In the present study, the subjects were in the age group of 25 to 50 years. Out of the total sample of 300 male bus drivers in Tricity, 159 reported that they had WRMSDs. The prevalence of WRMSDs among bus drivers in Tricity was 53%. In present study, the prevalence of low back pain was highest among the bus drivers that are 30.3%, then neck pain 17.3%, knee pain 14.7%, shoulder 6.3%, ankle and feet 5.7%, upper back 4%, hip and thigh 4%, elbow 1.3% and wrist and hand 1.3%. Thus low back pain, neck pain and knee pain are the most prevalent WRMSDs amongst bus drivers. Conclusions: Work-related biomechanical

  2. Step-by-step application methodology in practical KEPCO 22.9kV bus-bar system

    International Nuclear Information System (INIS)

    Yoon, Jae-young; Lee, Seung-yeol

    2010-01-01

    With the increase of power demand and the progress of power industry deregulation, the transmission and distribution systems will have more complicated problems by the influence of curtailing investment and the NIMBY phenomena in overall power systems. [1-2] It is expected that the route length per MW demand of South Korea will decrease gradually from 0.6[C-km/MW] to 0.53[C-km/MW] in 2010.[3] This comes up to a real serious problem from system planning and operation viewpoints. HTS technologies related to power system have properties to solve these complex transmission and distribution constraints, especially for metropolitan area, in the future. As the HTS technology has developed, the HTS cable technology can be the most effective alternative to solve the future expected power network constraints. This paper describes the application methodology of developing 22.9kV HTS cable by CAST for practical distribution system. 22.9kV HTS cable under development with step-by-step application methodology can substitute the existing and planning conventional 154kV cable.[4-5] If this scheme is applied, part of downtown 154kV substation of metropolitan city such as Seoul can be changed into 22.9kV switching station. Additionally, it can give huge economic, environmental benefits to all of the concerned authorities.

  3. Optimal bus temperature for thermal comfort during a cool day.

    Science.gov (United States)

    Velt, K B; Daanen, H A M

    2017-07-01

    A challenge for electric buses is to minimize heating and cooling power to maximally extend the driving range, but still provide sufficient thermal comfort for the driver and passengers. Therefore, we investigated the thermal sensation (TS) and thermal comfort (TC) of passengers in buses during a cool day (temperature 13.4 ± 0.5 °C, relative humidity (RH) 60 ± 5.8%) typical for the Dutch temperate maritime climate. 28 Males and 72 females rated TS and TC and gave information on age, stature, body weight and worn garments. The temperature in the bus of 22.5 ± 1.1 °C and RH of 59.9 ± 5.8% corresponded to a slightly warm feeling (TS = 0.85 ± 1.06) and TC of 0.39 ± 0.65. TS related significantly to bus temperature, clothing insulation and age. Linear regression based on these parameters showed that the temperature in the bus corresponding to TC = 0 and TS = 0 would have been 20.9 ± 0.6 °C. In conclusion, a 1.6 °C lower bus temperature during the investigated cool day probably would have led to less thermal discomfort and energy savings of electrical busses. The methodology to relate climatic measurements to subjective assessments is currently employed in a wider climatic range and may prove to be useful to find a better balance between thermal comfort and energy savings of the bus. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Boric Acid Reclamation System (BARS)

    International Nuclear Information System (INIS)

    Kniazewycz, B.G.; Markind, J.

    1986-01-01

    KLM Technologies was recently awarded a contract by the Department of Energy for a Phase II demonstration of an optimized full-scale prototype membrane system including performance evaluation under plant operating conditions. The program will serve as the catalyst for developing technology to augment the industry's incentive toward innovative and compact volume reduction alternatives for PWRs. The development and demonstration of the KLM Boric Acid Reclamation System, which is readily retrofitted into existing PWR facilities, will provide a positive means of reducing PWR waste volumes without requiring the $25-50 million equipment and support facility expenditures associated with most liquid waste volume reduction systems. This new application for membrane separation technology can reduce waste by upward of 50 percent for two-thirds of the operating nuclear plants in the U.S. The use of membrane technology has demonstrated significant process potential in radwaste and related applications. Reverse Osmosis (RO) and Ultrafiltration (UF) can provide selective filtration capability and concentrate contaminants without the need of filter aids, thus minimizing the requirements of chemical regeneration, costly resins, and major process equipment with large auxiliary heat supplies. KLM Technologies' personnel have identified a Boric Acid Reclamation System (BARS) utilizing RO and UF to produce a recyclable grade of otherwise waste boric acid at PWRs, thus reducing a major source of low-level radwaste. The design of a prototype BARS as a compact volume reduction system was the result of KLM's Phase I Program, and based upon a preliminary feasibility program, which assessed the applicability of membrane technology to refurbish and recycle waste boric acid from floor and equipment drain streams. The analysis of the overall program indicated a substantial savings regarding off-site disposal costs

  5. On the Relation between Spector's Bar Recursion and Modified Bar Recursion

    DEFF Research Database (Denmark)

    Oliva, Paulo Borges

    2002-01-01

    We introduce a variant of Spector's Bar Recursion in finite types to give a realizability interpretation of the classical axiom of dependent choice allowing for the extraction of witnesses from proofs of Sigma_1 formulas in classical analysis. We also give a bar recursive definition of the fan...... functional and study the relationship of our variant of Bar Recursion with others....

  6. Bank pull or bar push: What drives scroll-bar formation in meandering rivers?

    NARCIS (Netherlands)

    van de Lageweg, W. I.; van Dijk, W. M.; Baar, A. W.; Rutten, J.; Kleinhans, M. G.

    2014-01-01

    One of the most striking features of meandering rivers are quasi-regular ridges of the point bar, evidence of a pulsed lateral migration of meander bends. Scroll bars formed on the inner bend are preserved on the point-bar surface as a series of ridges as meanders migrate, and in the subsurface of

  7. Test of bar window with internal bars free from the glass surfaces

    DEFF Research Database (Denmark)

    Schultz, Jørgen Munthe

    1998-01-01

    A sealed glazing unit with 3 horisontal and 3 vertical bars and a reference glazing without bars have been tested in a guarded hotbox. The difference in measured heat loss coefficient between the two test objects is a measure of the thermal influence of the bars. The difference in heat loss...

  8. The cc-bar and bb-bar spectroscopy in the two-step potential model

    International Nuclear Information System (INIS)

    Kulshreshtha, D.S.; Kaiserslautern Univ.

    1984-07-01

    We investigate the spectroscopy of the charmonium (cc-bar) and bottonium (bb-bar) bound states in a static flavour independent nonrelativistic quark-antiquark (qq-bar) two-step potential model proposed earlier. Our predictions are in good agreement with experimental data and with other theoretical predictions. (author)

  9. Too Much Bar and Not Enough Mitzvah? A Proposed Research Agenda on Bar/Bat Mitzvah

    Science.gov (United States)

    Schoenfeld, Stuart

    2010-01-01

    Jewish educators are understandably interested in research on how bar/bat mitzvah affect Jewish education or research on what Jewish schools have done to avoid the distortions of a focus on bar/bat mitzvah. Research might also focus on the somewhat different and more ambitious topic of the role that bar/bat mitzvah play in contemporary Jewish…

  10. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2011-03-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. This report provides the early data results and implementation experience of the AT fuel cell bus since it was placed in service.

  11. Method of predicting Splice Sites based on signal interactions

    Directory of Open Access Journals (Sweden)

    Deogun Jitender S

    2006-04-01

    Full Text Available Abstract Background Predicting and proper ranking of canonical splice sites (SSs is a challenging problem in bioinformatics and machine learning communities. Any progress in SSs recognition will lead to better understanding of splicing mechanism. We introduce several new approaches of combining a priori knowledge for improved SS detection. First, we design our new Bayesian SS sensor based on oligonucleotide counting. To further enhance prediction quality, we applied our new de novo motif detection tool MHMMotif to intronic ends and exons. We combine elements found with sensor information using Naive Bayesian Network, as implemented in our new tool SpliceScan. Results According to our tests, the Bayesian sensor outperforms the contemporary Maximum Entropy sensor for 5' SS detection. We report a number of putative Exonic (ESE and Intronic (ISE Splicing Enhancers found by MHMMotif tool. T-test statistics on mouse/rat intronic alignments indicates, that detected elements are on average more conserved as compared to other oligos, which supports our assumption of their functional importance. The tool has been shown to outperform the SpliceView, GeneSplicer, NNSplice, Genio and NetUTR tools for the test set of human genes. SpliceScan outperforms all contemporary ab initio gene structural prediction tools on the set of 5' UTR gene fragments. Conclusion Designed methods have many attractive properties, compared to existing approaches. Bayesian sensor, MHMMotif program and SpliceScan tools are freely available on our web site. Reviewers This article was reviewed by Manyuan Long, Arcady Mushegian and Mikhail Gelfand.

  12. Bar Study Stories. Issues in Prevention

    Science.gov (United States)

    Higher Education Center for Alcohol, Drug Abuse, and Violence Prevention, 2012

    2012-01-01

    This issue of "Issues in Prevention" focuses on the impact of the availability of drinks in licensed establishments, such as bars and taverns on student drinking. This issue contains the following articles: (1) Cheap Drinks at College Bars Can Escalate Student Drinking (John D. Clapp); (2) High Alcohol Outlet Density: A Problem for Campuses and…

  13. Constraints on the Galactic bar with RAVE

    NARCIS (Netherlands)

    Antoja, T.; Helmi, A.; Helmi, [Unknown

    We derive the pattern speed of the Galactic bar from the analysis of the kinematics of the Hercules stream at different Galactocentric radii with RAVE, assuming that Hercules is caused by the bar. We find a well constrained pattern speed of Ωb=1.98+0.04 -0.08 Ωo, where Ω0 is the local circular

  14. Needle bar for warp knitting machines

    Science.gov (United States)

    Hagel, Adolf; Thumling, Manfred

    1979-01-01

    Needle bar for warp knitting machines with a number of needles individually set into slits of the bar and having shafts cranked to such an extent that the head section of each needle is in alignment with the shaft section accommodated by the slit. Slackening of the needles will thus not influence the needle spacing.

  15. Why Are Some Galaxies Not Barred?

    Science.gov (United States)

    Saha, Kanak; Elmegreen, Bruce

    2018-05-01

    Although more than two-thirds of star-forming disk galaxies in the local universe are barred, some galaxies remain unbarred, occupying the upper half of the Hubble tuning fork diagram. Numerical simulations almost always produce bars spontaneously, so it remains a challenge to understand how galaxies sometimes prevent bars from forming. Using a set of collisionless simulations, we first reproduce the common result that cold stellar disks surrounding a classical bulge become strongly unstable to non-axisymmetric perturbations, leading to the rapid formation of spiral structure and bars. However, our analyses show that galaxy models with compact classical bulges (whose average density is greater than or comparable to the disk density calculated within bulge half-mass radii) are able to prevent bar formation for at least 4 Gyr even when the stellar disk is maximal and having low Toomre Q. Such bar prevention is the result of several factors such as (a) a small inner Lindblad resonance with a high angular rate, which contaminates an incipient bar with x 2 orbits, and (b) rapid loss of angular momentum accompanied by a rapid heating in the center from initially strong bar and spiral instabilities in a low-Q disk; in other words, a rapid initial rise to a value larger than ∼5 of the ratio of the random energy to the rotational energy in the central region of the galaxy.

  16. The Bar Tack Machine. Module 16.

    Science.gov (United States)

    South Carolina State Dept. of Education, Columbia. Office of Vocational Education.

    This module on the bar tack machine, one in a series dealing with industrial sewing machines, their attachments, and operation, covers one topic: performing special operations on the bar tack machine. These components are provided: an introduction, directions, an objective, learning activities, student information, a student self-check, and a…

  17. Statistical analysis of LHC main interconnection splices room temperature resistance (R-8) results

    CERN Document Server

    Heck, S

    2012-01-01

    During the 2008/2009 shutdown the so-called R-8/R-16 room temperature resistance test has been introduced for the quality control of the LHC main interconnection splices. It has been found that at present two groups of LHC main interconnection splices can be distinguished, so-called “old” splices produced during LHC installation, and so-called “new” splices produced during 2009. 2009 production splices are considered as the state-of-the art, which is reflected by a much smaller R-8 distribution as compared to that of splices produced during first LHC installation.

  18. Rancang Bangun Sistem Pemesanan Tiket Bus Kupang-Atambua Berbasis SMS Gateway

    Directory of Open Access Journals (Sweden)

    Emerensiana Ngaga

    2016-11-01

    Full Text Available Bus merupakan alat transportasi yang paling banyak digunakan masyarakat karena harga yang murah dan masih terjangkau untuk masyarakat kalangan ekonomi menengah ke bawah.  Umumnya pemesanan tiket bus dilakukan dengan mendatangi langsung tempat penjualan tiket bus atau dengan cara menelepon ke kantor agen. Proses pemesanan seperti ini  memiliki kekurangan dimana pegawai kerepotan dalam mencatat dan seringkali terjadi kesalahan dalam mencatat pemesanan tiket. Sedangkan untuk waktu pemesanan, umumnya calon penumpang melakukan pemesanan tiket, satu atau dua hari sebelum keberangkatan dan ada juga yang tidak sempat memesan tiket beberapa hari sebelumnya dan melakukan pembelian tiket pada hari keberangkatan sehingga sering kali tidak mendapatkan tiket. Penelitian ini bertujuan merancang bangun sistem pemesanan tiket bus berbasis SMS Gateway yang memberikan kemudahan layanan pemesanan tiket bus bagi masyarakat secara jarak jauh serta kemudahan mengetahui informasi penjadwalan bus dengan cepat. Aplikasi dibangun menggunakan metode Unified Process, dengan bahasa pemrograman Java NetBeansIDE 7.3.1, MySQL sebagai database dan Gammu sebagai software untuk membantu pembangunan aplikasi SMS Gateway. Hasil dari penelitian ini adalah sebuah sistem pemesanan tiket bus yang memberikan alternatif baru proses pemesanan tiket bus secara baik tanpa ada permasalahan jarak dan waktu. Selain itu masyarakat juga dapat mengetahui informasi jadwal bus, harga tiket dan juga informasi bila terjadi perubahan jadwal keberangkatan bus.   Kata kunci— Bus, Tiket, SMS Gateway, Unified Process

  19. Deciphering the BAR code of membrane modulators.

    Science.gov (United States)

    Salzer, Ulrich; Kostan, Julius; Djinović-Carugo, Kristina

    2017-07-01

    The BAR domain is the eponymous domain of the "BAR-domain protein superfamily", a large and diverse set of mostly multi-domain proteins that play eminent roles at the membrane cytoskeleton interface. BAR domain homodimers are the functional units that peripherally associate with lipid membranes and are involved in membrane sculpting activities. Differences in their intrinsic curvatures and lipid-binding properties account for a large variety in membrane modulating properties. Membrane activities of BAR domains are further modified and regulated by intramolecular or inter-subunit domains, by intermolecular protein interactions, and by posttranslational modifications. Rather than providing detailed cell biological information on single members of this superfamily, this review focuses on biochemical, biophysical, and structural aspects and on recent findings that paradigmatically promote our understanding of processes driven and modulated by BAR domains.

  20. Orbits in weak and strong bars

    CERN Document Server

    Contopoulos, George

    1980-01-01

    The authors study the plane orbits in simple bar models embedded in an axisymmetric background when the bar density is about 1% (weak), 10% (intermediate) or 100% (strong bar) of the axisymmetric density. Most orbits follow the stable periodic orbits. The basic families of periodic orbits are described. In weak bars with two Inner Lindblad Resonances there is a family of stable orbits extending from the center up to the Outer Lindblad Resonance. This family contains the long period orbits near corotation. Other stable families appear between the Inner Lindblad Resonances, outside the Outer Lindblad Resonance, around corotation (short period orbits) and around the center (retrograde). Some families become unstable or disappear in strong bars. A comparison is made with cases having one or no Inner Lindblad Resonance. (12 refs).

  1. Characterization of Bars Induced by Interactions

    Directory of Open Access Journals (Sweden)

    Inma Martinez-Valpuesta

    2016-05-01

    Full Text Available Whether the formation of bars is triggered by interactions or by internal processes has been discussed for many decades. In this work, we study differences between both mechanisms by means of numerical simulations. We relate our analysis to fly-by interactions in different mass groups or clusters according to the velocity of the encounters. We find that once the bar is created, the interaction does not much affect its evolution. We also find that bars can be triggered purely by a slow interaction. Those bars affected or triggered by interaction stay for a longer time in the slow regime, i.e., the corotation radius is more than 1.4 times the bar radius.

  2. Charmonium and bottomonium in bar pp interactions

    International Nuclear Information System (INIS)

    Pordes, S.

    1993-12-01

    In this talk, I presented some examples of data from the CDF collaboration on J/ψ, χ, ψ' and Γ production. Such data are used to test models of production dynamics and for the understanding of rates for b quark production. I am not a member of the CDF experiment and showed their data with permission as an interested and impressed spectator. Data from D0 may be found in the talk of D. Denisov. As a complement to this data from the highest energy accelerator experiment, operating at √ bar s= 1.8 TeV, I also showed data from Fermilab experiment E760 on masses, widths, states and branching ratios in the Charmonium system, obtained by studying resonant formation of c bar c states in p bar p annihilation at √ bar s = m(c bar c)

  3. Expandable antivibration bar for a steam generator

    International Nuclear Information System (INIS)

    Lagally, H.O.

    1987-01-01

    This patent describes a steam generator for a nuclear power plant comprising a shell, a plurality of tubes having a U-shaped configuration arranged in successive columns within the shell. The tubes are adapted to heat feedwater flowing around the outside of the tubes by the flow of hot reactor coolant within the tubes, and antivibration bars any vibrations of the tubes as a result of steam between the columns of tubes. The improvement described here comprises means for varying the thickness of the antivibration bars to fit substantially the actual space between the columns of tubes comprising first and second bars, with at least one bar being movable, and with at least one mating inclined surface between the first and second bars

  4. A Study of (bar B)0 --> D(*)0 (bar K)(*)0 Decays

    International Nuclear Information System (INIS)

    Aubert, B.

    2004-01-01

    The authors presented evidence for the decay (bar B) 0 --> D* 0 (bar K) 0 as well as new measurements of the branching fractions for the decays (bar B) 0 --> D 0 (bar K) 0 and D 0 (bar K)* 0 . Their measurements are in agreement with the expectation derived from a cited reference and with previous measurements. They use the central value of their measurement for B((bar B) 0 --> (bar D) 0 K* 0 ) and obtain τ < 0.8 at the 90% C.L. from a central value of τ = 0.4 ± 0.2 (stat.) ± 0.2 (syst.). The main contribution to the systematic uncertainty is from the estimated peaking background since most systematic uncertainties on the branching fractions cancel in the ratio

  5. Formation of q bar q resonances in the bar NN system

    International Nuclear Information System (INIS)

    Ivanov, N.Ya.

    1995-01-01

    The formation of q bar q resonances lying on the leading Regge trajectories in the bar NN system is studied in the quark-gluon string model. The model predicts strong suppression of the decays of q bar q states into bar NN pairs in relation to two-meson modes. The author's analysis shows that the contributions of the resonances f 4 (2050) (I G J PC = 0 + 4 ++ ), ρ 5 (2240) (I G J PC = 1 + 5 -- ), and f 6 (2510) (I G J PC = 0 + 6 ++ ) to the processes of two-meson bar NN annihilation (bar pp → ππ, bar KK, hor-ellipsis) are about 1% of the corresponding experimental integrated cross sections. 30 refs., 2 figs., 1 tab

  6. Human Splice-Site Prediction with Deep Neural Networks.

    Science.gov (United States)

    Naito, Tatsuhiko

    2018-04-18

    Accurate splice-site prediction is essential to delineate gene structures from sequence data. Several computational techniques have been applied to create a system to predict canonical splice sites. For classification tasks, deep neural networks (DNNs) have achieved record-breaking results and often outperformed other supervised learning techniques. In this study, a new method of splice-site prediction using DNNs was proposed. The proposed system receives an input sequence data and returns an answer as to whether it is splice site. The length of input is 140 nucleotides, with the consensus sequence (i.e., "GT" and "AG" for the donor and acceptor sites, respectively) in the middle. Each input sequence model is applied to the pretrained DNN model that determines the probability that an input is a splice site. The model consists of convolutional layers and bidirectional long short-term memory network layers. The pretraining and validation were conducted using the data set tested in previously reported methods. The performance evaluation results showed that the proposed method can outperform the previous methods. In addition, the pattern learned by the DNNs was visualized as position frequency matrices (PFMs). Some of PFMs were very similar to the consensus sequence. The trained DNN model and the brief source code for the prediction system are uploaded. Further improvement will be achieved following the further development of DNNs.

  7. RNA splicing in a new rhabdovirus from Culex mosquitoes.

    Science.gov (United States)

    Kuwata, Ryusei; Isawa, Haruhiko; Hoshino, Keita; Tsuda, Yoshio; Yanase, Tohru; Sasaki, Toshinori; Kobayashi, Mutsuo; Sawabe, Kyoko

    2011-07-01

    Among members of the order Mononegavirales, RNA splicing events have been found only in the family Bornaviridae. Here, we report that a new rhabdovirus isolated from the mosquito Culex tritaeniorhynchus replicates in the nuclei of infected cells and requires RNA splicing for viral mRNA maturation. The virus, designated Culex tritaeniorhynchus rhabdovirus (CTRV), shares a similar genome organization with other rhabdoviruses, except for the presence of a putative intron in the coding region for the L protein. Molecular phylogenetic studies indicated that CTRV belongs to the family Rhabdoviridae, but it is yet to be assigned a genus. Electron microscopic analysis revealed that the CTRV virion is extremely elongated, unlike virions of rhabdoviruses, which are generally bullet shaped. Northern hybridization confirmed that a large transcript (approximately 6,500 nucleotides [nt]) from the CTRV L gene was present in the infected cells. Strand-specific reverse transcription-PCR (RT-PCR) analyses identified the intron-exon boundaries and the 76-nt intron sequence, which contains the typical motif for eukaryotic spliceosomal intron-splice donor/acceptor sites (GU-AG), a predicted branch point, and a polypyrimidine tract. In situ hybridization exhibited that viral RNAs are primarily localized in the nucleus of infected cells, indicating that CTRV replicates in the nucleus and is allowed to utilize the host's nuclear splicing machinery. This is the first report of RNA splicing among the members of the family Rhabdoviridae.

  8. A Challenging Pie to Splice: Drugging the Spliceosome.

    Science.gov (United States)

    León, Brian; Kashyap, Manoj K; Chan, Warren C; Krug, Kelsey A; Castro, Januario E; La Clair, James J; Burkart, Michael D

    2017-09-25

    Since its discovery in 1977, the study of alternative RNA splicing has revealed a plethora of mechanisms that had never before been documented in nature. Understanding these transitions and their outcome at the level of the cell and organism has become one of the great frontiers of modern chemical biology. Until 2007, this field remained in the hands of RNA biologists. However, the recent identification of natural product and synthetic modulators of RNA splicing has opened new access to this field, allowing for the first time a chemical-based interrogation of RNA splicing processes. Simultaneously, we have begun to understand the vital importance of splicing in disease, which offers a new platform for molecular discovery and therapy. As with many natural systems, gaining clear mechanistic detail at the molecular level is key towards understanding the operation of any biological machine. This minireview presents recent lessons learned in this emerging field of RNA splicing chemistry and chemical biology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Two new splice variants in porcine PPARGC1A

    Directory of Open Access Journals (Sweden)

    Peelman Luc J

    2008-12-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptor γ coactivator 1α (PPARGC1A is a coactivator with a vital and central role in fat and energy metabolism. It is considered to be a candidate gene for meat quality in pigs and is involved in the development of obesity and diabetes in humans. How its many functions are regulated, is however still largely unclear. Therefore a transcription profile of PPARGC1A in 32 tissues and 4 embryonic developmental stages in the pig was constructed by screening its cDNA for possible splice variants with exon-spanning primers. Findings This led to the discovery of 2 new splice variants in the pig, which were subsequently also detected in human tissues. In these variants, exon 8 was either completely or partly (the last 66 bp were conserved spliced out, potentially coding for a much shorter protein of respectively 337 and 359 amino acids (aa, of which the first 291 aa would be the same compared to the complete protein (796 aa. Conclusion Considering the functional domains of the PPARGC1A protein, it is very likely these splice variants considerably affect the function of the protein and alternative splicing could be one of the mechanisms by which the diverse functions of PPARGC1A are regulated.

  10. The determinants of alternative RNA splicing in human cells.

    Science.gov (United States)

    Ramanouskaya, Tatsiana V; Grinev, Vasily V

    2017-12-01

    Alternative splicing represents an important level of the regulation of gene function in eukaryotic organisms. It plays a critical role in virtually every biological process within an organism, including regulation of cell division and cell death, differentiation of tissues in the embryo and the adult organism, as well as in cellular response to diverse environmental factors. In turn, studies of the last decade have shown that alternative splicing itself is controlled by different mechanisms. Unfortunately, there is no clear understanding of how these diverse mechanisms, or determinants, regulate and constrain the set of alternative RNA species produced from any particular gene in every cell of the human body. Here, we provide a consolidated overview of alternative splicing determinants including RNA-protein interactions, epigenetic regulation via chromatin remodeling, coupling of transcription-to-alternative splicing, effect of secondary structures in pre-RNA, and function of the RNA quality control systems. We also extensively and critically discuss some mechanistic insights on coordinated inclusion/exclusion of exons during the formation of mature RNA molecules. We conclude that the final structure of RNA is pre-determined by a complex interplay between cis- and trans-acting factors. Altogether, currently available empirical data significantly expand our understanding of the functioning of the alternative splicing machinery of cells in normal and pathological conditions. On the other hand, there are still many blind spots that require further deep investigations.

  11. The Carnegie-Irvine Galaxy Survey. V. Statistical Study of Bars and Buckled Bars

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhao-Yu [Key Laboratory for Research in Galaxies and Cosmology, Shanghai Astronomical Observatory, Chinese Academy of Science, 80 Nandan Road, Shanghai 200030 (China); Ho, Luis C. [Kavli Institute for Astronomy and Astrophysics, Peking University, Beijing 100871 (China); Barth, Aaron J., E-mail: lizy@shao.ac.cn [Department of Physics and Astronomy, 4129 Frederick Reines Hall, University of California, Irvine, CA, 92697-4575 (United States)

    2017-08-10

    Simulations have shown that bars are subject to a vertical buckling instability that transforms thin bars into boxy or peanut-shaped structures, but the physical conditions necessary for buckling to occur are not fully understood. We use the large sample of local disk galaxies in the Carnegie-Irvine Galaxy Survey to examine the incidence of bars and buckled bars across the Hubble sequence. Depending on the disk inclination angle ( i ), a buckled bar reveals itself as either a boxy/peanut-shaped bulge (at high i ) or as a barlens structure (at low i ). We visually identify bars, boxy/peanut-shaped bulges, and barlenses, and examine the dependence of bar and buckled bar fractions on host galaxy properties, including Hubble type, stellar mass, color, and gas mass fraction. We find that the barred and unbarred disks show similar distributions in these physical parameters. The bar fraction is higher (70%–80%) in late-type disks with low stellar mass ( M {sub *} < 10{sup 10.5} M {sub ⊙}) and high gas mass ratio. In contrast, the buckled bar fraction increases to 80% toward massive and early-type disks ( M {sub *} > 10{sup 10.5} M {sub ⊙}), and decreases with higher gas mass ratio. These results suggest that bars are more difficult to grow in massive disks that are dynamically hotter than low-mass disks. However, once a bar forms, it can easily buckle in the massive disks, where a deeper potential can sustain the vertical resonant orbits. We also find a probable buckling bar candidate (ESO 506−G004) that could provide further clues to understand the timescale of the buckling process.

  12. The Reduction of CO2 Emissions by Application of High-Strength Reinforcing Bars to Three Different Structural Systems in South Korea

    Directory of Open Access Journals (Sweden)

    Seungho Cho

    2017-09-01

    Full Text Available The architecture, engineering, and construction (AEC industry consume approximately 23% of the national energy annually, and are considered among the highest energy consuming industries. Recently, several studies have focused on establishing strategies to reduce the emissions of carbon dioxide in the AEC industry by utilisation of low-carbon materials, material reuse, recycling and minimal usage; selection of an optimal structural system and structural optimisation; and optimisation of construction operations. While several studies examined material selection and replacement in concrete, there is a paucity of studies investigating the replacement and implementation of high-strength re-bars to lower the carbon dioxide emissions in buildings. To fill this research gap, the purpose of this study involves calculating the emissions of carbon dioxide by applying high-strength reinforcement bars in three different types of buildings. The input–output analysis method was adopted to compute the emissions of carbon dioxide by using the yield strength and size. This study showed that the application of the high-strength re-bars is beneficial in reducing the input amount of materials, although the quantity of reinforcing bars on the development and splice increased. Furthermore, the application of high-strength deformed bars is also advantageous as a means of carbon dioxide reduction in the studied structural systems. In this study, the CO2 emissions of three different structural systems indicated that implementing SD500 re-bars is the most effective method to reduce carbon dioxide emissions.

  13. The bar in NGC 4596

    International Nuclear Information System (INIS)

    Kent, S.M.

    1990-01-01

    The SBa galaxy NGC 4596 is characterized on the basis of CCD photometry obtained with a broad red filter on the 61-cm telescope at Whipple Observatory during January 1986 and long-slit CCD spectra obtained with the 4-m telescope at KPNO in May 1988 and with the MMT in March 1989. The results are presented graphically and analyzed in detail. Three components are identified: (1) an oblate spheroidal bulge with true ellipticity 0.26 and luminosity 4.7 x 10 to the 9th solar luminosities, (2) a 10.0 x 2.6-kpc rectangular bar with luminosity 6.7 x 10 to the 9th solar luminosities, and (3) a lens of constant intensity with luminosity 3.9 x 10 to the 9th solar luminosities out to a distance of 8.7 kpc. The characteristic slowdown time is calculated as 6-20 Gyr, and the velocity field is shown to deviate less from circular rotation than predicted by a simple dynamical model in which the disk kinematics are derived from an n-body simulation (Sparkle and Sellwood, 1987) and the bulge is assumed to be an oblate isotropic rotator. 31 refs

  14. Modified sine bar device measures small angles with high accuracy

    Science.gov (United States)

    Thekaekara, M.

    1968-01-01

    Modified sine bar device measures small angles with enough accuracy to calibrate precision optical autocollimators. The sine bar is a massive bar of steel supported by two cylindrical rods at one end and one at the other.

  15. American Fuel Cell Bus Project Evaluation: Third Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jeffers, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2017-05-01

    This report presents results of the American Fuel Cell Bus (AFCB) Project, a demonstration of fuel cell electric buses operating in the Coachella Valley area of California. The prototype AFCB, which was developed as part of the Federal Transit Administration's (FTA) National Fuel Cell Bus Program, was delivered to SunLine in November 2011 and was put in revenue service in mid-December 2011. Two new AFCBs with an upgraded design were delivered in June/July of 2014 and a third new AFCB was delivered in February 2015. FTA and the AFCB project team are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory to evaluate the buses in revenue service. This report covers the performance of the AFCBs from July 2015 through December 2016.

  16. Discharging a DC bus capacitor of an electrical converter system

    Science.gov (United States)

    Kajouke, Lateef A; Perisic, Milun; Ransom, Ray M

    2014-10-14

    A system and method of discharging a bus capacitor of a bidirectional matrix converter of a vehicle are presented here. The method begins by electrically shorting the AC interface of the converter after an AC energy source is disconnected from the AC interface. The method continues by arranging a plurality of switching elements of a second energy conversion module into a discharge configuration to establish an electrical current path from a first terminal of an isolation module, through an inductive element, and to a second terminal of the isolation module. The method also modulates a plurality of switching elements of a first energy conversion module, while maintaining the discharge configuration of the second energy conversion module, to at least partially discharge a DC bus capacitor.

  17. Distributed cooperative synchronization strategy for multi-bus microgrids

    DEFF Research Database (Denmark)

    Sun, Yao; Zhong, Chaolu; Hou, Xiaochao

    2017-01-01

    Microgrids can operate in both grid-connected mode and islanded mode. In order to smooth transfer from islanded mode to grid-connected mode, it is necessary to synchronize the point of common coupling (PCC) with main utility grid (UG) in voltage frequency, phase and amplitude. Conventional...... synchronization methods based on centralized communication are very costly and not suitable for multi-bus microgrids that have a large number of distributed generators (DGs). To address this concern, this study presents an active synchronization control strategy based on distributed cooperation technology...... for multi-bus microgrids. The proposed method can reconnect the microgrid in island to UG seamlessly with sparse communication channels. Synchronization correction signals are generated by a voltage controller, which are only transmitted to the leader DGs. Meanwhile, each DG exchanges information with its...

  18. BAR domain proteins regulate Rho GTPase signaling.

    Science.gov (United States)

    Aspenström, Pontus

    2014-01-01

    BAR proteins comprise a heterogeneous group of multi-domain proteins with diverse biological functions. The common denominator is the Bin-Amphiphysin-Rvs (BAR) domain that not only confers targeting to lipid bilayers, but also provides scaffolding to mold lipid membranes into concave or convex surfaces. This function of BAR proteins is an important determinant in the dynamic reconstruction of membrane vesicles, as well as of the plasma membrane. Several BAR proteins function as linkers between cytoskeletal regulation and membrane dynamics. These links are provided by direct interactions between BAR proteins and actin-nucleation-promoting factors of the Wiskott-Aldrich syndrome protein family and the Diaphanous-related formins. The Rho GTPases are key factors for orchestration of this intricate interplay. This review describes how BAR proteins regulate the activity of Rho GTPases, as well as how Rho GTPases regulate the function of BAR proteins. This mutual collaboration is a central factor in the regulation of vital cellular processes, such as cell migration, cytokinesis, intracellular transport, endocytosis, and exocytosis.

  19. Bar Formation in Milky Way type Galaxies

    Directory of Open Access Journals (Sweden)

    Polyachenko E. V.

    2016-12-01

    Full Text Available Many barred galaxies, possibly including the Milky Way, have cusps in their centers. There is a widespread belief, however, that the usual bar instability, which occurs in bulgeless galaxy models, is impossible for cuspy models because of the presence of the inner Lindblad resonance for any pattern speed. At the same time, there is numerical evidence that the bar instability can form a bar. We analyze this discrepancy by performing accurate and diverse N-body simulations and calculating the normal modes. We show that bar formation in cuspy galaxies can be explained by taking into account the disk thickness. The exponential growth time is moderate (about 250 Myr for typical current disk masses, but it increases considerably (by a factor of two or more if the live halo and bulge are substituted by a rigid halo/bulge potential; the pattern speeds remain almost the same. Normal mode analysis with different disk mass favors a young bar hypothesis, according to which the bar instability has saturated only recently.

  20. Pre-mRNA mis-splicing of sarcomeric genes in heart failure.

    Science.gov (United States)

    Zhu, Chaoqun; Chen, Zhilong; Guo, Wei

    2017-08-01

    Pre-mRNA splicing is an important biological process that allows production of multiple proteins from a single gene in the genome, and mainly contributes to protein diversity in eukaryotic organisms. Alternative splicing is commonly governed by RNA binding proteins to meet the ever-changing demands of the cell. However, the mis-splicing may lead to human diseases. In the heart of human, mis-regulation of alternative splicing has been associated with heart failure. In this short review, we focus on alternative splicing of sarcomeric genes and review mis-splicing related heart failure with relatively well studied Sarcomeric genes and splicing mechanisms with identified regulatory factors. The perspective of alternative splicing based therapeutic strategies in heart failure has also been discussed. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Alternative splicing: the pledge, the turn, and the prestige : The key role of alternative splicing in human biological systems.

    Science.gov (United States)

    Gallego-Paez, L M; Bordone, M C; Leote, A C; Saraiva-Agostinho, N; Ascensão-Ferreira, M; Barbosa-Morais, N L

    2017-09-01

    Alternative pre-mRNA splicing is a tightly controlled process conducted by the spliceosome, with the assistance of several regulators, resulting in the expression of different transcript isoforms from the same gene and increasing both transcriptome and proteome complexity. The differences between alternative isoforms may be subtle but enough to change the function or localization of the translated proteins. A fine control of the isoform balance is, therefore, needed throughout developmental stages and adult tissues or physiological conditions and it does not come as a surprise that several diseases are caused by its deregulation. In this review, we aim to bring the splicing machinery on stage and raise the curtain on its mechanisms and regulation throughout several systems and tissues of the human body, from neurodevelopment to the interactions with the human microbiome. We discuss, on one hand, the essential role of alternative splicing in assuring tissue function, diversity, and swiftness of response in these systems or tissues, and on the other hand, what goes wrong when its regulatory mechanisms fail. We also focus on the possibilities that splicing modulation therapies open for the future of personalized medicine, along with the leading techniques in this field. The final act of the spliceosome, however, is yet to be fully revealed, as more knowledge is needed regarding the complex regulatory network that coordinates alternative splicing and how its dysfunction leads to disease.

  2. Foundation field bus in the Spanish nuclear sector

    International Nuclear Information System (INIS)

    Saez de Montagut Revenga, G.

    2013-01-01

    This paper deals with the modification made in power plants nuclear ASCO 1 and 2 in which scans is the level Control system of Heaters to implementing an Ovation system, as well as the standard Foundation Field bus for field communications. This digitalisation has required a detailed study of the implications on security that could have the centralization of control loops in main feedwater system.

  3. BC Transit Fuel Cell Bus Project: Evaluation Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, M. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-02-01

    This report evaluates a fuel cell electric bus demonstration led by British Columbia Transit (BC Transit) in Whistler, Canada. BC Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's National Renewable Energy Laboratory to evaluate the buses in revenue service. This evaluation report covers two years of revenue service data on the buses from April 2011 through March 2013.

  4. The Designing Bus for Nuclear Safety Class Controller

    International Nuclear Information System (INIS)

    Lee, Dongil; Lee, Myeongkyun; Yun, Donghwa; Ryoo, Kwangki

    2013-01-01

    EtherCAT (Ethernet for Control Automation Technology) is based on the IEEE 802.3 standard as one of the communication which is the I/O (Input/Output), sensors and communication function of PLC (Programmable Logic Controller) in industry and factory environment use is increasing. The Nuclear Safety Class Controller implemented by the EtherCAT applied bus can be shown the improving performance of data transmission in the controller

  5. Deregulation and Intercity Bus Operations in Florida: A Preliminary Study.

    Science.gov (United States)

    1981-03-02

    revenues have been consistent recently, but expenses have risen. The three areas most frequently served by Rabbit Bus Lines are Disney World, Sea World...Manager, Shoreline Stages had annual revenues of roughly $890,000 in 1980. In terms of trips, about ninety percent of this carrier’s traffic is interstate...this traffic constitutes the bulk of Shoreline Stage’s business by revenue , volume of traffic, and number of passengers. Charter trips to Disney

  6. Parallel data grabbing card based on PCI bus RS422

    International Nuclear Information System (INIS)

    Zhang Zhenghui; Shen Ji; Wei Dongshan; Chen Ziyu

    2005-01-01

    This article briefly introduces the developments of the parallel data grabbing card based on RS422 and PCI bus. It could be applied for grabbing the 14 bits parallel data in high speed, coming from the devices with RS422 interface. The methods of data acquisition which bases on the PCI protocol, the functions and their usages of the chips employed, the ideas and principles of the hardware and software designing are presented. (authors)

  7. The Designing Bus for Nuclear Safety Class Controller

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dongil; Lee, Myeongkyun; Yun, Donghwa [PONUTech Co,. Ltd., Research Institute, Ulsan (Korea, Republic of); Ryoo, Kwangki [Hanbat National Univ., Daejeon (Korea, Republic of)

    2013-10-15

    EtherCAT (Ethernet for Control Automation Technology) is based on the IEEE 802.3 standard as one of the communication which is the I/O (Input/Output), sensors and communication function of PLC (Programmable Logic Controller) in industry and factory environment use is increasing. The Nuclear Safety Class Controller implemented by the EtherCAT applied bus can be shown the improving performance of data transmission in the controller.

  8. Low back trouble among urban bus drivers in Denmark

    DEFF Research Database (Denmark)

    Netterstrøm, Bo; Juel, K

    1989-01-01

    herniation was 137 compared to all Danish men. These differences were statistically significant. Of psychosocial factors only "the feeling of being mentally unbalanced" was found to be statistically significantly related to subsequent hospital discharge with a LBT-diagnosis. Long seniority as bus driver...... of those discharged from hospital with LBT makes it probable that LBT has etiologic factors in the work environment. The sedentary position and whole-body vibrations may be contributory causes....

  9. A counting-card circuit based on PCI bus

    International Nuclear Information System (INIS)

    Shi Jing; Li Yong; Chinese Academy of Sciences, Lanzhou; Su Hong; Dong Chengfu; Li Xiaogang; Ma Xiaoli

    2004-01-01

    A counting-card circuit based on PCI bus that we developed recently used for advanced personal computer will be introduced in this paper briefly. The maximum count capacity of this counting-card is 10 9 -1, ranging from 0 to 999 999 999, the maximum counting time range, 1 x 10 6 s, can be set in 1 cycle, the maximum counting rate is 20 MHz for positive input. (authors)

  10. Oxidative and nitrosative stress markers in bus drivers

    Czech Academy of Sciences Publication Activity Database

    Rössner ml., Pavel; Švecová, Vlasta; Milcová, Alena; Lněničková, Zdena; Solanský, I.; Santella, R. M.; Šrám, Radim

    2007-01-01

    Roč. 617, - (2007), s. 23-32 ISSN 0027-5107 R&D Projects: GA MŽP SL/5/160/05; GA AV ČR 1QS500390506 Institutional research plan: CEZ:AV0Z50390512 Keywords : oxidative stress * bus drivers * air pollution Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 4.159, year: 2007

  11. Multi drop bus controller for IBM PC/AT

    International Nuclear Information System (INIS)

    Bardik, Yu.I.; Kalistratov, E.N.; Matyushin, A.A.; Obukhov, G.A.; Trofimov, N.N.

    1993-01-01

    The module PC-AT-PBC gives a possibility to create a working place based on the computer IBM PC/AT for testing of hardware which is compatible with the multi drop bus. The KP1830BE31 micro program change turns the module into timing generator for power supply controllers of the UNK correction system. The structure of the module, functional parts, library functions and two application programs have been described. 4 refs., 9 figs

  12. Definition and Evaluation of Bus and Truck Automation Operations Concepts

    OpenAIRE

    Tsao, H.-S. Jacob; Botha, Jan L.

    2002-01-01

    Traffic congestion will continue to worsen and likely worsen at a faster rate than ever. People throughput and freight throughput have become critical issues for California and the rest of the nation. PATH has been funding a one-year research project entitled "Definition and Evaluation of Bus and Truck Automation Operations Concepts," proposed by the authors. This report summarizes the results of the research project achieved during the first six months. During those six months, we reviewed l...

  13. A Modern Picture of Barred Galaxy Dynamics

    Science.gov (United States)

    Petersen, Michael; Weinberg, Martin; Katz, Neal

    2018-01-01

    Observations of disk galaxies suggest that bars are responsible for altering global galaxy parameters (e.g. structures, gas fraction, star formation rate). The canonical understanding of the mechanisms underpinning bar-driven secular dynamics in disk galaxies has been largely built upon the analysis of linear theory, despite galactic bars being clearly demonstrated to be nonlinear phenomena in n-body simulations. We present simulations of barred Milky Way-like galaxy models designed to elucidate nonlinear barred galaxy dynamics. We have developed two new methodologies for analyzing n-body simulations that give the best of both powerful analytic linear theory and brute force simulation analysis: orbit family identification and multicomponent torque analysis. The software will be offered publicly to the community for their own simulation analysis.The orbit classifier reveals that the details of kinematic components in galactic disks (e.g. the bar, bulge, thin disk, and thick disk components) are powerful discriminators of evolutionary paradigms (i.e. violent instabilities and secular evolution) as well as the basic parameters of the dark matter halo (mass distribution, angular momentum distribution). Multicomponent torque analysis provides a thorough accounting of the transfer of angular momentum between orbits, global patterns, and distinct components in order to better explain the underlying physics which govern the secular evolution of barred disk galaxies.Using these methodologies, we are able to identify the successes and failures of linear theory and traditional n-body simulations en route to a detailed understanding of the control bars exhibit over secular evolution in galaxies. We present explanations for observed physical and velocity structures in observations of barred galaxies alongside predictions for how structures will vary with dynamical properties from galaxy to galaxy as well as over the lifetime of a galaxy, finding that the transfer of angular

  14. Widespread evolutionary conservation of alternatively spliced exons in caenorhabditis

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob L; Penny, David

    2007-01-01

    Alternative splicing (AS) contributes to increased transcriptome and proteome diversity in various eukaryotic lineages. Previous studies showed low levels of conservation of alternatively spliced (cassette) exons within mammals and within dipterans. We report a strikingly different pattern...... in Caenorhabditis nematodes-more than 92% of cassette exons from Caenorhabditis elegans are conserved in Caenorhabditis briggsae and/or Caenorhabditis remanei. High levels of conservation extend to minor-form exons (present in a minority of transcripts) and are particularly pronounced for exons showing complex...... patterns of splicing. The functionality of the vast majority of cassette exons is underscored by various other features. We suggest that differences in conservation between lineages reflect differences in levels of functionality and further suggest that these differences are due to differences in intron...

  15. Body Temperature Cycles Control Rhythmic Alternative Splicing in Mammals.

    Science.gov (United States)

    Preußner, Marco; Goldammer, Gesine; Neumann, Alexander; Haltenhof, Tom; Rautenstrauch, Pia; Müller-McNicoll, Michaela; Heyd, Florian

    2017-08-03

    The core body temperature of all mammals oscillates with the time of the day. However, direct molecular consequences of small, physiological changes in body temperature remain largely elusive. Here we show that body temperature cycles drive rhythmic SR protein phosphorylation to control an alternative splicing (AS) program. A temperature change of 1°C is sufficient to induce a concerted splicing switch in a large group of functionally related genes, rendering this splicing-based thermometer much more sensitive than previously described temperature-sensing mechanisms. AS of two exons in the 5' UTR of the TATA-box binding protein (Tbp) highlights the general impact of this mechanism, as it results in rhythmic TBP protein levels with implications for global gene expression in vivo. Together our data establish body temperature-driven AS as a core clock-independent oscillator in mammalian peripheral clocks. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Genome-wide survey of allele-specific splicing in humans

    Directory of Open Access Journals (Sweden)

    Scheffler Konrad

    2008-06-01

    Full Text Available Abstract Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation on the efficiency of mRNA splicing is often difficult to predict, many mutations that cause disease through an effect on splicing are likely to remain undiscovered. Results We have combined a genome-wide scan for sequence polymorphisms likely to affect mRNA splicing with analysis of publicly available Expressed Sequence Tag (EST and exon array data. The genome-wide scan uses published tools and identified 30,977 SNPs located within donor and acceptor splice sites, branch points and exonic splicing enhancer elements. For 1,185 candidate splicing polymorphisms the difference in splicing between alternative alleles was corroborated by publicly available exon array data from 166 lymphoblastoid cell lines. We developed a novel probabilistic method to infer allele-specific splicing from EST data. The method uses SNPs and alternative mRNA isoforms mapped to EST sequences and models both regulated alternative splicing as well as allele-specific splicing. We have also estimated heritability of splicing and report that a greater proportion of genes show evidence of splicing heritability than show heritability of overall gene expression level. Our results provide an extensive resource that can be used to assess the possible effect on splicing of human polymorphisms in putative splice-regulatory sites. Conclusion We report a set of genes showing evidence of allele-specific splicing from an integrated analysis of genomic polymorphisms, EST data and exon array

  17. Determining vertical bar Vub vertical bar from the B-bar→Xulν-bar dilepton invariant mass spectrum

    International Nuclear Information System (INIS)

    Bauer, Christian W.; Ligeti, Zoltan; Luke, Michael

    2001-01-01

    The invariant mass spectrum of the lepton pair in inclusive semileptonic B-bar→X u lν-bar decay yields a model independent determination of vertical bar V ub vertical bar. Unlike the lepton energy and hadronic invariant mass spectra, nonperturbative effects are only important in the resonance region, and play a parametrically suppressed role when dΓ/dq 2 is integrated over q 2 >(m B -m D ) 2 , which is required to eliminate the B-bar→X c lν-bar background. We discuss these backgrounds for q 2 slightly below (m B -m D ) 2 , and point out that instead of q 2 >(m B -m D ) 2 =11.6 GeV 2 , the cut can be lowered to q 2 > or approx. 10.5 GeV 2 . This is important experimentally, particularly when effects of a finite neutrino reconstruction resolution are included

  18. First Measurement of σ(gg → t$\\bar{t}$)/σ(p$\\bar{p}$ → t$\\bar{t}$)

    Energy Technology Data Exchange (ETDEWEB)

    Alamdari, Shabnaz Pashapour [Univ. of Toronto, ON (Canada)

    2008-01-01

    The work presented here is the first measurement of the fraction of top quark pair production through gluon-gluon fusion. We use an integrated luminosity of 0.96 ± 0.06 fb-1 of p{bar p} collisions at √s of 1.96 TeV collected by the CDF II detector. We select t$\\bar{t}$ candidates by identifying a high-pT lepton candidate, a large missing ET as evidence for a neutrino candidate and at least four high ET jets, one of which has to be identified as originating from a b quark. The challenge is to discriminate between the two production processes with the identical final state, gg → t$\\bar{t}$ and q$\\bar{p}$ → t$\\bar{t}$. We take advantage of the fact that compared to a quark, a gluon is more likely to radiate a low momentum gluon and therefore, one expects a larger number of charged particles with low pT in a process involving more gluons. Given the large uncertainties associated with the modeling of the low pT charged particle multiplicity, a data-driven technique was employed. Using calibration data samples, we show there exists a clear correlation between the observed average number of low pT charged particles and the average number of gluons involved in the production process predicted by Monte Carlo calculations. Given the correlation, one can identify low pT charged particle multiplicity distributions associated with specific average number of gluons. The W + 0 jet sample and dijets sample with leading jet ET in the range of 80-100 GeV are used to find no-gluon and gluon-rich low p{sub T} charged particle multiplicity distributions, respectively. Using these no-gluon and gluon-rich distributions in a likelihood fit, we find the fraction of gluon-rich events in t{bar t} candidates. This fraction has contributions from the signal and background events. Taking into account these contributions and the gg → t$\\bar{t}$ and q$\\bar{q}$ → t$\\bar

  19. Evaluation of Bus Vibration Comfort Based on Passenger Crowdsourcing Mode

    Directory of Open Access Journals (Sweden)

    Hong Zhao

    2016-01-01

    Full Text Available Vibration comfort is an important factor affecting the quality of service (QoS of bus. In order to make people involved in supervising bus’s vibration comfort and improve passengers’ riding experience, a novel mode of passenger crowdsourcing is introduced. In this paper, comfort degree of bus vibration is calculated from bus’s vibration signals collected by passengers’ smartphones and sent through WiFi to the Boa web server which shows the vibration comfort on the LCD deployed in bus and maybe trigger alarm lamp when the vibration is beyond the threshold. Three challenges here have been overcome: firstly, space coordinate transformation algorithm is used to solve the constant drift of signals collected; secondly, a low-pass filter is designed to isolate gravity from signals real-timely via limited computing resources; thirdly, an embedded evaluation system is developed according to the calculation procedure specified by criterion ISO 2631-1997. Meanwhile, the model proposed is tested in a practical running environment, the vibration data in whole travel are recorded and analyzed offline. The results show that comfort degree of vibration obtained from the experimental system is identical with the truth, and this mode is proved to be effective.

  20. HEV Test Bench Based on CAN Bus Sensor Communication

    Directory of Open Access Journals (Sweden)

    Shupeng ZHAO

    2014-02-01

    Full Text Available The HEV test bench based on Controller Area Network bus was studied and developed. Control system of HEV power test bench used the CAN bus technology. The application of CAN bus technology on control system development has opened up a new research direction for domestic automobile experimental platform. The HEV power control system development work was completed, including power master controller, electric throttle controller, driving simulation platform, CAN2.0 B communication protocol procedures for formulation, CAN communication monitoring system, the simulation model based on MATLAB code automatic generation technology research, etc. Maximum absorption power of the test bench is 90 kW, the test bench top speed is 6000 r/min, the CAN communication data baud rate is 10~500 k, the conventional electric measurement parameter part precision satisfies the requirement of development of HEV. On the HEV test bench the result of regenerative braking experiment shows that the result got by the test bench was closer to the results got by outdoor road test. And the fuel consumption experiment test results show that the HEV fuel consumption and the charge-discharge character are in linear relationship. The establishment of the test platform for the evaluation of the development of hybrid electric vehicle and power provides physical simulation and test platform.

  1. Electric bus migration in Bengaluru with dynamic charging technologies

    Directory of Open Access Journals (Sweden)

    Ankit Kumar Begwani

    2017-12-01

    Full Text Available Rapid but unplanned urban development coupled with economic growth has resulted in congestion and pollution concerns in Indian cities. This forced India consider taking concrete steps towards design policies that would help stir the nation towards a more sustainable future. India, along with other bigger Asian economies like China, needs to address the growing global concerns over climate change and design their framework and policies to help cut down the greenhouse gas emissions. Transportation is a major source of pollution. A shift towards a pro-public transport policy would solve the traffic congestion problems and address the emission concerns. India has a significantly higher modal share of public transportation in its major cities. Public bus transport system acts as a lifeline to the India’s poor and middle class citizens. The following study focuses on the need for a replacement of conventional fossil fuel dependent buses with Electric buses in the existing public transport bus fleet in the city of Bengaluru. A design has been developed to utilize wireless charging technologies to realize electric bus migration in Bengaluru.

  2. Pilot survey of subway and bus stop noise levels.

    Science.gov (United States)

    Gershon, Robyn R M; Neitzel, Richard; Barrera, Marissa A; Akram, Muhammad

    2006-09-01

    Excessive noise exposure is a serious global urban health problem, adversely affecting millions of people. One often cited source of urban noise is mass transit, particularly subway systems. As a first step in determining risk within this context, we recently conducted an environmental survey of noise levels of the New York City transit system. Over 90 noise measurements were made using a sound level meter. Average and maximum noise levels were measured on subway platforms, and maximum levels were measured inside subway cars and at several bus stops for comparison purposes. The average noise level measured on the subway platforms was 86 +/- 4 dBA (decibel-A weighting). Maximum levels of 106, 112, and 89 dBA were measured on subway platforms, inside subway cars, and at bus stops, respectively. These results indicate that noise levels in subway and bus stop environments have the potential to exceed recommended exposure guidelines from the World Health Organization (WHO) and U.S. Environmental Protection Agency (EPA), given sufficient exposure duration. Risk reduction strategies following the standard hierarchy of control measures should be applied, where feasible, to reduce subway noise exposure.

  3. Replacement model of city bus: A dynamic programming approach

    Science.gov (United States)

    Arifin, Dadang; Yusuf, Edhi

    2017-06-01

    This paper aims to develop a replacement model of city bus vehicles operated in Bandung City. This study is driven from real cases encountered by the Damri Company in the efforts to improve services to the public. The replacement model propounds two policy alternatives: First, to maintain or keep the vehicles, and second is to replace them with new ones taking into account operating costs, revenue, salvage value, and acquisition cost of a new vehicle. A deterministic dynamic programming approach is used to solve the model. The optimization process was heuristically executed using empirical data of Perum Damri. The output of the model is to determine the replacement schedule and the best policy if the vehicle has passed the economic life. Based on the results, the technical life of the bus is approximately 20 years old, while the economic life is an average of 9 (nine) years. It means that after the bus is operated for 9 (nine) years, managers should consider the policy of rejuvenation.

  4. Safety evaluation of a hydrogen fueled transit bus

    Energy Technology Data Exchange (ETDEWEB)

    Coutts, D.A.; Thomas, J.K.; Hovis, G.L.; Wu, T.T. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1997-12-31

    Hydrogen fueled vehicle demonstration projects must satisfy management and regulator safety expectations. This is often accomplished using hazard and safety analyses. Such an analysis has been completed to evaluate the safety of the H2Fuel bus to be operated in Augusta, Georgia. The evaluation methods and criteria used reflect the Department of Energy`s graded approach for qualifying and documenting nuclear and chemical facility safety. The work focused on the storage and distribution of hydrogen as the bus motor fuel with emphases on the technical and operational aspects of using metal hydride beds to store hydrogen. The safety evaluation demonstrated that the operation of the H2Fuel bus represents a moderate risk. This is the same risk level determined for operation of conventionally powered transit buses in the United States. By the same criteria, private passenger automobile travel in the United States is considered a high risk. The evaluation also identified several design and operational modifications that resulted in improved safety, operability, and reliability. The hazard assessment methodology used in this project has widespread applicability to other innovative operations and systems, and the techniques can serve as a template for other similar projects.

  5. Violence against metropolitan bus drivers and fare collectors in Brazil

    Directory of Open Access Journals (Sweden)

    Ada Ávila Assunção

    2015-01-01

    Full Text Available OBJECTIVE To analyze the correlation between sociodemographic factors and working conditions of bus workers in a metropolitan area and violence against them. METHODS This cross-sectional study used a nonprobabilistic sample estimated according to the number of workers employed in bus companies located in three cities in the Belo Horizonte metropolitan region in 2012 (N = 17,470. Face-to-face interviews were conducted using a digital questionnaire. The factors associated with violence were analyzed in two stages using Poisson regression, according to each level. The magnitude of the association was evaluated using prevalence ratios with robust variance and a statistical significance of 5%, and 95% confidence intervals were obtained. RESULTS The study sample comprised 782 drivers and 691 fare collectors; 45.0% participants reported at least one act of violence in the workplace in the last 12 months, with passengers being predominantly responsible. The age of the bus workers was inversely associated with violence. Chronic diseases, sickness absenteeism, and working conditions were also associated with violence. CONCLUSIONS The findings on the correlation between violence and working conditions are essential for implementing prevention strategies by transportation service managers.

  6. An Optimization Model for the Selection of Bus-Only Lanes in a City.

    Science.gov (United States)

    Chen, Qun

    2015-01-01

    The planning of urban bus-only lane networks is an important measure to improve bus service and bus priority. To determine the effective arrangement of bus-only lanes, a bi-level programming model for urban bus lane layout is developed in this study that considers accessibility and budget constraints. The goal of the upper-level model is to minimize the total travel time, and the lower-level model is a capacity-constrained traffic assignment model that describes the passenger flow assignment on bus lines, in which the priority sequence of the transfer times is reflected in the passengers' route-choice behaviors. Using the proposed bi-level programming model, optimal bus lines are selected from a set of candidate bus lines; thus, the corresponding bus lane network on which the selected bus lines run is determined. The solution method using a genetic algorithm in the bi-level programming model is developed, and two numerical examples are investigated to demonstrate the efficacy of the proposed model.

  7. An Optimization Model for the Selection of Bus-Only Lanes in a City.

    Directory of Open Access Journals (Sweden)

    Qun Chen

    Full Text Available The planning of urban bus-only lane networks is an important measure to improve bus service and bus priority. To determine the effective arrangement of bus-only lanes, a bi-level programming model for urban bus lane layout is developed in this study that considers accessibility and budget constraints. The goal of the upper-level model is to minimize the total travel time, and the lower-level model is a capacity-constrained traffic assignment model that describes the passenger flow assignment on bus lines, in which the priority sequence of the transfer times is reflected in the passengers' route-choice behaviors. Using the proposed bi-level programming model, optimal bus lines are selected from a set of candidate bus lines; thus, the corresponding bus lane network on which the selected bus lines run is determined. The solution method using a genetic algorithm in the bi-level programming model is developed, and two numerical examples are investigated to demonstrate the efficacy of the proposed model.

  8. Cellular Automata Based Modeling for Evaluating Different Bus Stop Designs in China

    Directory of Open Access Journals (Sweden)

    Haoyang Ding

    2015-01-01

    Full Text Available A cellular automaton model is proposed to simulate mixed traffic flow composed of motor vehicles and bicycles near bus stops. Three typical types of bus stops which are common in China are considered in the model, including two types of curbside bus stops and one type of bus bay stops. Passenger transport capacity of three types of bus stops, which is applied to evaluate the bus stop design, is calculated based on the corresponding traffic flow rate. According to the simulation results, the flow rates of both motor vehicles and bicycles exhibit phase transition from free flow to the saturation one at the critical point. The results also show that the larger the interaction between motor vehicle and bicycle flow is near curbside bus stops, the more the value of saturated flows drops. Curbside bus stops are more suitable when the conflicts between two flows are small and the inflow rate of motor vehicles is low. On the contrary, bus bay stops should be applied due to their ability to reduce traffic conflicts. Findings of this study can provide useful suggestions on bus stop selection considering different inflow rate of motor vehicles and bicycles simultaneously.

  9. What makes the family of barred disc galaxies so rich: damping stellar bars in spinning haloes

    Science.gov (United States)

    Collier, Angela; Shlosman, Isaac; Heller, Clayton

    2018-05-01

    We model and analyse the secular evolution of stellar bars in spinning dark matter (DM) haloes with the cosmological spin λ ˜ 0-0.09. Using high-resolution stellar and DM numerical simulations, we focus on angular momentum exchange between stellar discs and DM haloes of various axisymmetric shapes - spherical, oblate, and prolate. We find that stellar bars experience a diverse evolution that is guided by the ability of parent haloes to absorb angular momentum, J, lost by the disc through the action of gravitational torques, resonant and non-resonant. We confirm that dynamical bar instability is accelerated via resonant J-transfer to the halo. Our main findings relate to the long-term secular evolution of disc-halo systems: with an increasing λ, bars experience less growth and basically dissolve after they pass through vertical buckling instability. Specifically, with increasing λ, (1) the vertical buckling instability in stellar bars colludes with inability of the inner halo to absorb J - this emerges as the main factor weakening or destroying bars in spinning haloes; (2) bars lose progressively less J, and their pattern speeds level off; (3) bars are smaller, and for λ ≳ 0.06 cease their growth completely following buckling; (4) bars in λ > 0.03 haloes have ratio of corotation-to-bar radii, RCR/Rb > 2, and represent so-called slow bars without offset dust lanes. We provide a quantitative analysis of J-transfer in disc-halo systems, and explain the reasons for absence of growth in fast spinning haloes and its observational corollaries. We conclude that stellar bar evolution is substantially more complex than anticipated, and bars are not as resilient as has been considered so far.

  10. A novel splicing mutation in the V2 vasopressin receptor

    DEFF Research Database (Denmark)

    Kamperis, Konstantinos; Siggaard, C; Herlin, Troels

    2000-01-01

    as clinical investigations comprising a fluid deprivation test and a 1-deamino-8-D-arginine-vasopressin (dDAVP) infusion test in the study subject and his mother. We found a highly unusual, novel, de novo 1447A-->C point mutation (gDNA), involving the invariable splice acceptor of the second intron...... of the gene in both the affected male (hemizygous) and his mother (heterozygous). This mutation is likely to cause aberrant splicing of the terminal intron of the gene, leading to a non-functional AVP receptor. The clinical studies were consistent with such a hypothesis, as the affected subject had a severe...

  11. Working conditions of bus drivers in the private sector and bus crashes in Kandy district, Sri Lanka: a case-control study.

    Science.gov (United States)

    Jayatilleke, A U; Nakahara, S; Dharmaratne, S D; Jayatilleke, A C; Poudel, K C; Jimba, M

    2009-04-01

    To explore the effects of working conditions of private-bus drivers on bus crashes in Kandy district, Sri Lanka. A case-control study was carried out from August to September 2006. All private-bus drivers registered in Kandy district and involved in crashes reported to the police between November 2005 and April 2006 (n = 63) were selected as cases. Two control groups were included: private-bus drivers working on the same routes as the case drivers (n = 90) and private-bus drivers selected randomly from other routes of the district (n = 111). Data were collected using an anonymous self-administered questionnaire. Associations between working conditions and crashes were analysed using logistic regression. A strong association was observed between drivers' disagreements about working hours and bus crashes (matched controls, adjusted odds ratio (AOR) 5.98, 95% CI 1.02 to 34.90; unmatched controls, AOR 18.74, 95% CI 2.00 to 175.84). A significant association was also observed between low salaries (US$100) and private-bus crashes (matched controls, AOR 1.01, 95% CI 0.40 to 2.54; unmatched controls, AOR 3.09, 95% CI 1.26 to 7.57). Drivers' disagreements about working hours and low salaries were significant risk factors for private-bus crashes in Kandy district, Sri Lanka. Therefore, new legislation for private-bus owners on the working hours and salaries of their drivers to prevent private-bus crashes is recommended.

  12. DEDB: a database of Drosophila melanogaster exons in splicing graph form

    Directory of Open Access Journals (Sweden)

    Tan Tin

    2004-12-01

    Full Text Available Abstract Background A wealth of quality genomic and mRNA/EST sequences in recent years has provided the data required for large-scale genome-wide analysis of alternative splicing. We have capitalized on this by constructing a database that contains alternative splicing information organized as splicing graphs, where all transcripts arising from a single gene are collected, organized and classified. The splicing graph then serves as the basis for the classification of the various types of alternative splicing events. Description DEDB http://proline.bic.nus.edu.sg/dedb/index.html is a database of Drosophila melanogaster exons obtained from FlyBase arranged in a splicing graph form that permits the creation of simple rules allowing for the classification of alternative splicing events. Pfam domains were also mapped onto the protein sequences allowing users to access the impact of alternative splicing events on domain organization. Conclusions DEDB's catalogue of splicing graphs facilitates genome-wide classification of alternative splicing events for genome analysis. The splicing graph viewer brings together genome, transcript, protein and domain information to facilitate biologists in understanding the implications of alternative splicing.

  13. Interplay between DMD point mutations and splicing signals in Dystrophinopathy phenotypes.

    Directory of Open Access Journals (Sweden)

    Jonàs Juan-Mateu

    Full Text Available DMD nonsense and frameshift mutations lead to severe Duchenne muscular dystrophy while in-frame mutations lead to milder Becker muscular dystrophy. Exceptions are found in 10% of cases and the production of alternatively spliced transcripts is considered a key modifier of disease severity. Several exonic mutations have been shown to induce exon-skipping, while splice site mutations result in exon-skipping or activation of cryptic splice sites. However, factors determining the splicing pathway are still unclear. Point mutations provide valuable information regarding the regulation of pre-mRNA splicing and elements defining exon identity in the DMD gene. Here we provide a comprehensive analysis of 98 point mutations related to clinical phenotype and their effect on muscle mRNA and dystrophin expression. Aberrant splicing was found in 27 mutations due to alteration of splice sites or splicing regulatory elements. Bioinformatics analysis was performed to test the ability of the available algorithms to predict consequences on mRNA and to investigate the major factors that determine the splicing pathway in mutations affecting splicing signals. Our findings suggest that the splicing pathway is highly dependent on the interplay between splice site strength and density of regulatory elements.

  14. Analysis and recognition of 5 ' UTR intron splice sites in human pre-mRNA

    DEFF Research Database (Denmark)

    Eden, E.; Brunak, Søren

    2004-01-01

    Prediction of splice sites in non-coding regions of genes is one of the most challenging aspects of gene structure recognition. We perform a rigorous analysis of such splice sites embedded in human 5' untranslated regions (UTRs), and investigate correlations between this class of splice sites and...

  15. ISVASE: identification of sequence variant associated with splicing event using RNA-seq data.

    Science.gov (United States)

    Aljohi, Hasan Awad; Liu, Wanfei; Lin, Qiang; Yu, Jun; Hu, Songnian

    2017-06-28

    Exon recognition and splicing precisely and efficiently by spliceosome is the key to generate mature mRNAs. About one third or a half of disease-related mutations affect RNA splicing. Software PVAAS has been developed to identify variants associated with aberrant splicing by directly using RNA-seq data. However, it bases on the assumption that annotated splicing site is normal splicing, which is not true in fact. We develop the ISVASE, a tool for specifically identifying sequence variants associated with splicing events (SVASE) by using RNA-seq data. Comparing with PVAAS, our tool has several advantages, such as multi-pass stringent rule-dependent filters and statistical filters, only using split-reads, independent sequence variant identification in each part of splicing (junction), sequence variant detection for both of known and novel splicing event, additional exon-exon junction shift event detection if known splicing events provided, splicing signal evaluation, known DNA mutation and/or RNA editing data supported, higher precision and consistency, and short running time. Using a realistic RNA-seq dataset, we performed a case study to illustrate the functionality and effectiveness of our method. Moreover, the output of SVASEs can be used for downstream analysis such as splicing regulatory element study and sequence variant functional analysis. ISVASE is useful for researchers interested in sequence variants (DNA mutation and/or RNA editing) associated with splicing events. The package is freely available at https://sourceforge.net/projects/isvase/ .

  16. [Genetic diagnostics of pathogenic splicing abnormalities in the clinical laboratory--pitfalls and screening approaches].

    Science.gov (United States)

    Niimi, Hideki; Ogawa, Tomomi; Note, Rhougou; Hayashi, Shirou; Ueno, Tomohiro; Harada, Kenu; Uji, Yoshinori; Kitajima, Isao

    2010-12-01

    In recent years, genetic diagnostics of pathogenic splicing abnormalities are increasingly recognized as critically important in the clinical genetic diagnostics. It is reported that approximately 10% of pathogenic mutations causing human inherited diseases are splicing mutations. Nonetheless, it is still difficult to identify splicing abnormalities in routine genetic diagnostic settings. Here, we studied two different kinds of cases with splicing abnormalities. The first case is a protein S deficiency. Nucleotide analyses revealed that the proband had a previously reported G to C substitution in the invariant AG dinucleotide at the splicing acceptor site of intronl/exon2, which produces multiple splicing abnormalities resulting in protein S deficiency. The second case is an antithrombin (AT) deficiency. This proband had a previously reported G to A substitution, at nucleotide position 9788 in intron 4, 14 bp in front of exon 5, which created a de novo exon 5 splice site and resulted in AT deficiency. From a practical standpoint, we discussed the pitfalls, attentions, and screening approaches in genetic diagnostics of pathogenic splicing abnormalities. Due to the difficulty with full-length sequence analysis of introns, and the lack of RNA samples, splicing mutations may escape identification. Although current genetic testing remains to be improved, to screen for splicing abnormalities more efficiently, it is significant to use an appropriate combination of various approaches such as DNA and/or RNA samples, splicing mutation databases, bioinformatic tools to detect splice sites and cis-regulatory elements, and in vitro and/or in vivo experimentally methods as needed.

  17. Systematic profiling of alternative splicing signature reveals prognostic predictor for ovarian cancer.

    Science.gov (United States)

    Zhu, Junyong; Chen, Zuhua; Yong, Lei

    2018-02-01

    The majority of genes are alternatively spliced and growing evidence suggests that alternative splicing is modified in cancer and is associated with cancer progression. Systematic analysis of alternative splicing signature in ovarian cancer is lacking and greatly needed. We profiled genome-wide alternative splicing events in 408 ovarian serous cystadenocarcinoma (OV) patients in TCGA. Seven types of alternative splicing events were curated and prognostic analyses were performed with predictive models and splicing network built for OV patients. Among 48,049 mRNA splicing events in 10,582 genes, we detected 2,611 alternative splicing events in 2,036 genes which were significant associated with overall survival of OV patients. Exon skip events were the most powerful prognostic factors among the seven types. The area under the curve of the receiver-operator characteristic curve for prognostic predictor, which was built with top significant alternative splicing events, was 0.937 at 2,000 days of overall survival, indicating powerful efficiency in distinguishing patient outcome. Interestingly, splicing correlation network suggested obvious trends in the role of splicing factors in OV. In summary, we built powerful prognostic predictors for OV patients and uncovered interesting splicing networks which could be underlying mechanisms. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Resonant-bar gravitational radiation antennas

    International Nuclear Information System (INIS)

    Blair, D.G.

    1987-01-01

    This paper reviews the concept of gravitational radiation, and describes the worldwide research programme for the development of high-sensitivity resonant-bar antennas which are aimed at detecting gravitational radiation from astrophysical sources. (author)

  19. Life cycle cost analysis of a car, a city bus and an intercity bus powertrain for year 2005 and 2020

    International Nuclear Information System (INIS)

    Hellgren, Jonas

    2007-01-01

    The international economy, in the beginning of the 20th century, is characterized by uncertainty about the supply and the price of oil. Together with the fast decrease of electrical propulsion component prices, it becomes more and more cost effective to develop vehicles with alternative powertrains. This paper focuses on two questions: Are alternative powertrains especially cost effective for specific applications?; How does an increased fossil fuel price influences the choose of powertrain? To assess these questions, a computer tool named THEPS, developed in a Ph.D. project, is used. Three applications and three scenarios are analysed. The applications, a car, a city bus and an intercity bus, are vehicles all assumed to operate in Sweden. One scenario represents year 2005, the other two year 2020. The two future scenarios are characterized by different fossil fuel prices. The study, presented in the paper, indicates that alternative powertrains can be competitive from a cost perspective, in some applications, already in year 2005. It is for example cost effective to equip a city bus, running in countries with a high fuel price, with a hybrid powertrain. The study also indicates that pure electric, hybrid and/or fuel cell cars will probably be a more cost effective choice than conventional cars in year 2020. Another indication is that it will not be clear which powertrain concept to choose. The reason is that many cost effective powertrain concepts will be offered. The best choice will depend on the application

  20. Ultrasound-Guided Bar Edge Labeling in the Perioperative Assessment of Nuss Bar Removal.

    Science.gov (United States)

    Incerti, Filippo; Bertocchini, Alessia; Ghionzoli, Marco; Messineo, Antonio

    2017-12-01

    Nuss bar removal after minimally invasive repair of pectus excavatum in patients where bar ends are not palpable, can be a challenging procedure for the surgeon; a blind dissection toward the bar edges may lead to intercostal vessels or deep intercostal muscle injuries. In this article, we describe a fast, repeatable, low-cost technique to detect bar edge and stabilizers. A perioperative scan is performed by means of a portable ultrasonograph a few minutes before the operation. The bar edge stabilizer is detected as a hyperechogenic image with a concentric crescent while the bar edge is detected as a hyperechogenic dashed line with net edges. The scan is performed, and the actual projection on the skin of the metal plaque bulk is then labeled on the patient's chest by an ink marker. We believe that this method may improve morbidity, operative time, and consequently, hospitalization length and costs.

  1. New results from Fermilab E866 (NuSea) for d-bar/u-bar

    International Nuclear Information System (INIS)

    Isenhower, L. D.

    1999-01-01

    The Fermilab dimuon experiment 866/NuSea measured Drell-Yan yields from an 800 GeV/c proton beam incident on liquid hydrogen and deuterium targets. Over 370,000 Drell-Yan muon pairs were recorded. From these data, the ratio of anti-down (d-bar) to anti-up (u-bar) quark distributions in the proton sea is determined over a wide range in Bjorken-x. A strong x dependence is observed in the ratio d-bar/u-bar, showing substantial enhancement of d-bar with respect to u-bar for x < 0.2. The results presented here for the full data sets confirm previously published results from E866 and are compared with parametrizations of parton distribution functions calculated both before and after the publication of the high-mass E866 data

  2. Intelligent Bar Chart Plagiarism Detection in Documents

    Directory of Open Access Journals (Sweden)

    Mohammed Mumtaz Al-Dabbagh

    2014-01-01

    Full Text Available This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR. By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts.

  3. Practical, Reliable Error Bars in Quantum Tomography

    OpenAIRE

    Faist, Philippe; Renner, Renato

    2015-01-01

    Precise characterization of quantum devices is usually achieved with quantum tomography. However, most methods which are currently widely used in experiments, such as maximum likelihood estimation, lack a well-justified error analysis. Promising recent methods based on confidence regions are difficult to apply in practice or yield error bars which are unnecessarily large. Here, we propose a practical yet robust method for obtaining error bars. We do so by introducing a novel representation of...

  4. Chocolate Bars Based on Human Nutritional Requirements

    OpenAIRE

    Robson , Anthony ,

    2013-01-01

    International audience; Key Points * The nutritional value of chocolate bars should be based on the nutritional value of the low energy dense late Paleolithic human diet to help reduce mental ill health, obesity, and other postprandial insults. * Current chocolate bars have a high energy density (>2 kcal/g). * Cocoa can be sweetened by the addition of calorie-free Purefruit™ (Tate & Lyle) monk fruit ( Siraitia grosvenorii ) extract. PUREFRUIT™ is approximately 200 times sweeter than sugar and...

  5. Intelligent bar chart plagiarism detection in documents.

    Science.gov (United States)

    Al-Dabbagh, Mohammed Mumtaz; Salim, Naomie; Rehman, Amjad; Alkawaz, Mohammed Hazim; Saba, Tanzila; Al-Rodhaan, Mznah; Al-Dhelaan, Abdullah

    2014-01-01

    This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR). By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts.

  6. Configurating computer-controlled bar system

    OpenAIRE

    Šuštaršič, Nejc

    2010-01-01

    The principal goal of my diploma thesis is creating an application for configurating computer-controlled beverages dispensing systems. In the preamble of my thesis I present the theoretical platform for point of sale systems and beverages dispensing systems, which are required for the understanding of the target problematics. As with many other fields, computer tehnologies entered the field of managing bars and restaurants quite some time ago. Basic components of every bar or restaurant a...

  7. Intelligent Bar Chart Plagiarism Detection in Documents

    Science.gov (United States)

    Al-Dabbagh, Mohammed Mumtaz; Salim, Naomie; Alkawaz, Mohammed Hazim; Saba, Tanzila; Al-Rodhaan, Mznah; Al-Dhelaan, Abdullah

    2014-01-01

    This paper presents a novel features mining approach from documents that could not be mined via optical character recognition (OCR). By identifying the intimate relationship between the text and graphical components, the proposed technique pulls out the Start, End, and Exact values for each bar. Furthermore, the word 2-gram and Euclidean distance methods are used to accurately detect and determine plagiarism in bar charts. PMID:25309952

  8. The BaBar silicon vertex tracker

    International Nuclear Information System (INIS)

    Bozzi, C.; Carassiti, V.; Ramusino, A. Cotta; Dittongo, S.; Folegani, M.; Piemontese, L.; Abbott, B.K.; Breon, A.B.; Clark, A.R.; Dow, S.; Fan, Q.; Goozen, F.; Hernikl, C.; Karcher, A.; Kerth, L.T.; Kipnis, I.; Kluth, S.; Lynch, G.; Levi, M.; Luft, P.; Luo, L.; Nyman, M.; Pedrali-Noy, M.; Roe, N.A.; Zizka, G.; Roberts, D.; Barni, D.; Brenna, E.; Defendi, I.; Forti, A.; Giugni, D.; Lanni, F.; Palombo, F.; Vaniev, V.; Leona, A.; Mandelli, E.; Manfredi, P.F.; Perazzo, A.; Re, V.; Angelini, C.; Batignani, G.; Bettarini, S.; Bondioli, M.; Bosi, F.; Calderini, G.; Carpinelli, M.; Dutra, F.; Forti, F.; Gagliardi, D.; Giorgi, M.A.; Lusiani, A.; Mammini, P.; Morganti, M.; Morsani, F.; Paoloni, E.; Profeti, A.; Rama, M.; Rampino, G.; Rizzo, G.; Sandrelli, F.; Simi, G.; Triggiani, G.; Tritto, S.; Vitale, R.; Burchat, P.; Cheng, C.; Kirkby, D.; Meyer, T.; Roat, C.; Bona, M.; Bianchi, F.; Daudo, F.; Girolamo, B. Di; Gamba, D.; Giraudo, G.; Grosso, P.; Romero, A.; Smol, A.; Trapani, P.; Zanin, D.; Bosisio, L.; Ricca, G. Della; Lanceri, L.; Pompili, A.; Poropat, P.; Prest, M.; Rastelli, C.; Vallazza, E.; Vuagnin, G.; Hast, C.; Potter, E.P.; Sharma, V.; Burke, S.; Callahan, D.; Campagnari, C.; Dahmes, B.; Eppich, A.; Hale, D.; Hall, K.; Hart, P.; Kuznetsova, N.; Kyre, S.; Levy, S.; Long, O.; May, J.; Richman, J.; Verkerke, W.; Witherell, M.; Beringer, J.; Eisner, A.M.; Frey, A.; Grillo, A.; Grothe, M.; Johnson, R.; Kroeger, W.; Lockman, W.; Pulliam, T.; Rowe, W.; Schmitz, R.; Seiden, A.; Spencer, E.; Turri, M.; Wilder, M.; Charles, E.; Elmer, P.; Nielsen, J.; Orejudos, W.; Scott, I.; Walsh, J.; Zobernig, H.

    2000-01-01

    The BaBar Silicon Vertex Tracker (SVT) is designed to provide the high-precision vertexing necessary for making measurements of CP violation at the SLAC B-Factory PEP-II. The instrument consists of five layers of double-sided silicon strip detectors and has been installed in the BaBar experiment and taking colliding beam data since May 1999. An overview of the design as well as performance and experience from the initial running will be presented

  9. A PRACTICAL STUDY ON APPLICABILITY OF THE INCENTIVE SUBSIDY TO BUS NETWORK IN KUMAMOTO CITY

    Science.gov (United States)

    Mizokami, Shoshi; Fujimi, Toshio; Hirano, Toshihiko

    Recently, the number of passenger of public bus services in Japan tends to decrease due to the motorization. This condition leads to a severe management situation, including in Kumamoto City. Since 1984, the deficit of bus companies operation in provision of bus services in Kumamoto has been covered by Kumamoto City Government on the basis of lines subsidy, and the city allocated a number of subsidy of about 200 million yen in 2007. Currently, The Kumamoto city government plans to reduce the amount of subsidy to bus companies by introducing the giving of incentive to bus companies in order to trim bus company deficit. This study aims to construct the mathematical model of the incentive reward and apply the model to realignment of buses lines networks in Kumamoto Metropolitan Area.

  10. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Third Results Reports

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2012-05-01

    This report describes operations at SunLine Transit Agency for their newest prototype fuel cell bus and five compressed natural gas (CNG) buses. In May 2010, SunLine began operating its sixth-generation hydrogen fueled bus, an Advanced Technology (AT) fuel cell bus that incorporates the latest design improvements to reduce weight and increase reliability and performance. The agency is collaborating with the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to evaluate the bus in revenue service. NREL has previously published two reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from July 2011 through January 2012.

  11. SunLine Transit Agency Advanced Technology Fuel Cell Bus Evaluation: Fourth Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2013-01-01

    SunLine Transit Agency, which provides public transit services to the Coachella Valley area of California, has demonstrated hydrogen and fuel cell bus technologies for more than 10 years. In May 2010, SunLine began demonstrating the advanced technology (AT) fuel cell bus with a hybrid electric propulsion system, fuel cell power system, and lithium-based hybrid batteries. This report describes operations at SunLine for the AT fuel cell bus and five compressed natural gas buses. The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) is working with SunLine to evaluate the bus in real-world service to document the results and help determine the progress toward technology readiness. NREL has previously published three reports documenting the operation of the fuel cell bus in service. This report provides a summary of the results with a focus on the bus operation from February 2012 through November 2012.

  12. MIL-STD-1553 dynamic bus controller/remote terminal hybrid set

    Science.gov (United States)

    Friedman, S. N.

    This paper describes the performance, physical and electrical requirements of a Dual Redundant BUS Interface Unit (BIU) acting as a BUS Controller Interface Unit (BCIU) or Remote Terminal Unit (RTU) between a Motorola 68000 VME BUS and MIL-STD-1553B Multiplex Data Bus. A discussion of how the BIU Hybrid set is programmed, and operates as a BCIU or RTU, will be included. This paper will review Dynamic Bus Control and other Mode Code capabilities. The BIU Hybrid Set interfaces to a 68000 Microprocessor with a VME Bus using programmed I/O transfers. This special interface will be discussed along with the internal Dual Access Memory (4K x 16) used to support the data exchanges between the CPU and the BIU Hybrid Set. The hybrid set's physical size and power requirements will be covered. This includes the present Double Eurocard the BIU function is presently being offered on.

  13. Identification of voltage stability condition of a power system using measurements of bus variables

    Directory of Open Access Journals (Sweden)

    Durlav Hazarika

    2014-12-01

    Full Text Available Several online methods were proposed for investigating the voltage stability condition of an interconnected power system using the measurements of voltage and current phasors at a bus. For this purpose, phasor measurement units (PMUs are used. A PMU is a device which measures the electrical waves on an electrical network, using a common time source (reference bus for synchronisation. This study proposes a method for online monitoring of voltage stability condition of a power system using measurements of bus variables namely – (i real power, (ii reactive power and (iii bus voltage magnitude at a bus. The measurements of real power, reactive power and bus voltage magnitude could be extracted/captured from a smart energy meter. The financial involvement for implementation of the proposed method would significantly lower compared with the PMU-based method.

  14. Bitmap-Wise Wireless M-Bus Coordination for Sustainable Real Time Energy Management

    Directory of Open Access Journals (Sweden)

    Kwang-il Hwang

    2014-07-01

    Full Text Available Even though WM-Bus is being considered to be the most promising network protocol for smart metering, it is not suitable for a sustainable real-time home energy management system (HEMS, which requires higher reliability and longer lifetime despite real time bi-directional communications. Therefore, in this paper we propose a Bitmap-wise WM-Bus (BWM-Bus, coping well with sustainable real-time HEMS. In particular, the proposed scheme addresses the several problems in WM-Bus for HEMS by introducing novel functions: asynchronous meter trigger, adaptive slot scheduling, and bitmap-wise retransmission request. Through experiments, we demonstrate that BWM-Bus guarantees higher data success ratio with lower data aggregation time, as well as longer lifetime than WM-Bus standard.

  15. Preparation of a Breadfruit Flour Bar.

    Science.gov (United States)

    Nochera, Carmen L; Ragone, Diane

    2016-05-20

    Breadfruit is a nutritious, high energy food with a low quantity of protein but excellent protein quality. It has the potential to be developed into desired products which will help increase its utilization and add value to the crop. The overall purposes of this investigation were to develop a portable, nutritious, ready-to-eat breadfruit product (bar), test the sensory qualities of the product, and evaluate the nutritional properties of the product. Flour made from the Micronesian variety, Meinpadahk ( Artocarpus altilis × Artocarpus mariannensis ), was utilized for the development of the breadfruit bar. Breadfruit is a rich source of fiber, vitamins such as vitamin C, minerals such as potassium, and phytochemicals such as flavonoids. Nutritional labeling indicates that the breadfruit bar is high in carbohydrates and low in fat, and sensory evaluation indicates that 81% of the panelists found the bar acceptable while 19% disliked the bar. The breadfruit bar can provide an appealing and inexpensive gluten-free food source based on locally available breadfruit.

  16. The hydrogen 700 project - 700 Bar Co

    International Nuclear Information System (INIS)

    Gambone, L.; Webster, C.

    2004-01-01

    'Full text:' Major automotive companies, including DaimlerChrysler, Ford, Hyundai, Nissan, PSA Peugeot-Citroen, and Toyota, are co-operating in the Hydrogen 700 project at Powertech to establish a global basis for high pressure hydrogen fuel systems for vehicles. The fuel systems will store compressed hydrogen on-board at pressures up to 700 bar (10,000psi). It is anticipated that the 700 bar storage pressure will provide hydrogen powered vehicles with a range comparable to the range of petroleum-fueled vehicles. The Hydrogen 700 project has contracted world leaders in high pressure technologies to provide 700 bar fuel system components for evaluation. The data from these tests will be used as the basis for the development of relevant standards and regulations. In a development that complements the Hydrogen 700 project, Powertech Labs has established the world's first 700 bar hydrogen station for fast filling operations. This prototype station will be used to evaluate the performance of the 700 bar vehicle fuel system components. The presentation will provide an overview of the Hydrogen 700 project. Safety issues surrounding the use of compressed hydrogen gas as a vehicle fuel, as well as the use of higher storage pressures, will be reviewed. Test data involving the fire testing of vehicles containing hydrogen fuel systems will be presented. The project is intended to result in the introduction of 700 bar fuel systems in the next generation of hydrogen powered vehicles. (author)

  17. A Model of Bus Bunching under Reliability-based Passenger Arrival Patterns

    OpenAIRE

    Fonzone, Achille; Schmöcker, Jan-Dirk; Liu, Ronghui

    2015-01-01

    If bus service departure times are not completely unknown to the passengers, non-uniform passenger arrival patterns can be expected. We propose that passengers decide their arrival time at stops based on a continuous logit model that considers the risk of missing services. Expected passenger waiting times are derived in a bus system that allows also for overtaking between bus services. We then propose an algorithm to derive the dwell time of subsequent buses serving a stop in order to illustr...

  18. The Bus Extension Module Design and Verification of POSAFE-Q Using LVDS

    International Nuclear Information System (INIS)

    Lee, Youn Sang; Song, Seung Whan; No, Young Hun; Yoo, Kwan Woo

    2012-01-01

    This paper described the Bus Extension Module using Low Voltage Differential Signal (LVDS). A Bus Extension Module use Safety-Related PLC (Programmable Logic Controller), called POSAFE-Q (made by POSCO ICT), for handling many data (I/O) extension rack. A processor module of POSAFE-Q can control I/O Module installed another rack. This paper explains Bus Extension Module and Data transfer technology using LVDS. (author)

  19. Small Explorer Data System MIL-STD-1773 fiber optic bus

    Science.gov (United States)

    Flanegan, Mark; Label, Ken

    1992-01-01

    The MIL-STD-1773 Fiber Optic Data Bus as implemented in the GSFC Small Explorer Data System (SEDS) for the Small Explorer Program is described. It provides an overview of the SEDS MIL-STD-1773 bus components system design considerations, reliability figures, acceptance and qualification testing requirements, radiation requirements and tests, error handling considerations, and component heritage. The first mission using the bus will be launched in June of 1992.

  20. Evaluation of Urban Bus Transport Failure in Gujranwala City of Pakistan

    Directory of Open Access Journals (Sweden)

    MUHAMMAD WAQAR ASLAM

    2017-07-01

    Full Text Available This research work has evaluated the performance of urban public bus transport service operating in Gujranwala city of Pakistan. The assessment has been made in terms of financial viability, bus condition, operational performance and user perception. Based on the data requirements, various traffic/transport surveys were conducted including Passenger Boarding and Alighting Survey, Bus Count Survey, Bus Inspection Survey, Illegal Operations Survey and Public Transport User Interview Survey in consultation with concerned department/agencies. Field surveys were also conducted to get input from passengers and local people. Initial investigation informed that out of four operational bus routes, only two routes are profitable. The overall bus operation is running in loss (only 0.3% net profit. It has barely any profit for the bus operator against its investment thereby making the urban bus operation financially non-viable. The whole revenue flow is dependent on passenger ticketing revenue only. Around 35% of the buses are in poor condition with major issues of quality of tyres, no speedometer, worse condition of seats, floor and ceiling, and display of route. Illegal operation of Qingqis/Rickshaws (as para-transit mode along the bus routes is also decreasing the revenue by capturing the passenger volume. According to passenger opinion analysis, around 55% of the passenger are not satisfied with the service quality and prefer other modes of transport. Their major concerns are absence of facilities for disables and bus stops, bus seating and standing capacity, poor bus condition and pick pocketing. Collectively, these factors are contributing