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Sample records for burster gs 1826-238

  1. Hard X-ray Tail Discovered in the Clocked Burster GS 1826-238

    CERN Document Server

    Rodi, James; Roques, Jean-Pierre

    2015-01-01

    The LMXB NS GS 1826-238 was discovered by Ginga in 1988 September. Due to the presence of quasi-periodicity in the type I X-ray burst rate, the source has been a frequent target of X-ray observations for almost 30 years. Though the bursts were too soft to be detected by INTEGRAL/SPI, the persistent emission from GS 1826-238 was detected over 150 keV during the ~10 years of observations. Spectral analysis found a significant high-energy excess above a Comptonization model that is well fit by a power law, indicating an additional spectral component. Most previously reported spectra with hard tails in LMXB NS have had an electron temperature of a few keV and a hard tail dominating above ~50 keV with an index of \\Gamma ~ 2-3. GS 1826-238 was found to have a markedly different spectrum with $ kT_e \\sim 20 $ keV and a hard tail dominating above ~150 keV with an index of \\Gamma ~ 1.8, more similar to BHXRB. We report on our search for long-term spectral variability over the 25-370 keV energy range and on a compariso...

  2. Chandra and RXTE Spectra of the Burster GS 1826-238

    CERN Document Server

    Thompson, T W J; Tomsick, J A; Marshall, H L

    2005-01-01

    Using simultaneous observations from Chandra and RXTE, we investigated the LMXB GS 1826-238 with the goal of studying its spectral and timing properties. The uninterrupted Chandra observation captured 6 bursts (RXTE saw 3 of the 6), yielding a recurrence time of 3.54 +/- 0.03 hr. Using the proportional counter array on board RXTE, we made a probable detection of 611 Hz burst oscillations in the decaying phases of the bursts with an average rms signal amplitude of 4.8%. The integrated persistent emission spectrum can be described as the dual Comptonization of ~ 0.3 keV soft photons by a plasma with kT_e ~ 20 keV and an optical depth of about 2.6 (interpreted as emission from the accretion disk corona), plus the Comptonization of hotter ~ 0.8 keV seed photons by a ~ 6.8 keV plasma (interpreted as emission from or near the boundary layer). We discovered evidence for a neutral Fe K\\alpha emission line, and we found interstellar Fe L_II and Fe L_III absorption features. The burst spectrum can be fit by fixing the ...

  3. The hard X-ray shortages prompted by the clock bursts in GS 1826-238

    International Nuclear Information System (INIS)

    We report on a study of GS 1826-238 using all available Rossi X-Ray Timing Explorer observations, concentrating on the behavior of the hard X-rays during type-I bursts. We find a hard X-ray shortage at 30-50 keV prompted by the shower of soft X-rays coming from type-I bursts. This shortage happens with a time delay after the peak of the soft flux of 3.6 ± 1.2 s. The behavior of hard X-rays during bursts indicates cooling and reheating of the corona, during which a large amount of energy is required. We speculate that this energy originates from the feedback of the type-I bursts to the accretion process, resulting in a rapid temporary increase of the accretion rate.

  4. A Suzaku observation of the low-mass X-ray binary GS 1826-238 in the hard state

    Science.gov (United States)

    Ono, Ko; Sakurai, Soki; Zhang, Zhongli; Nakazawa, Kazuhiro; Makishima, Kazuo

    2016-06-01

    The neutron star low-mass X-ray binary GS 1826-238 was observed with Suzaku on 2009 October 21, for a total exposure of 1030 ks. Except for the type I bursts, the source intensity was constant within ˜10%. Combining the Suzaku XIS, HXD-PIN, and HXD-GSO data, burst-removed persistent emission was detected over the 0.8-100 keV range, at an unabsorbed flux of 2.6 × 10-9 erg s-1 cm-2. Although the implied 0.8-100 keV luminosity, 1.5 × 1037 erg s-1 (assuming a distance of 7 kpc), is relatively high, the observed hard spectrum confirms that the source was in the hard state. The spectrum was successfully explained by an emission from a soft standard accretion disk partially Comptonized by a hot electron cloud, and a blackbody emission Comptonized by another hotter electron cloud. These results are compared with those from previous studies, including those on the same source by Thompson et al. (2005, ApJ, 634, 1261) and Cocchi, Farinelli, and Paizis (2011, A&A, 529, A155), as well as that of Aql X-1 in the hard state obtained with Suzaku (Sakurai et al. 2014, PASJ, 66, 10).

  5. New insights into the quasi-periodic X-ray burster GS 0836-429

    CERN Document Server

    Aranzana, E; Kuulkers, E

    2015-01-01

    GS 0836-429 is a neutron star X-ray transient that displays Type-I X-ray bursts. In 2003 and 2004 it experienced two outbursts in X-rays. We present here an analysis of the system bursting properties during these outbursts. We studied the evolution of the 2003-2004 outbursts in soft X-rays using RXTE (2.5-12 keV; ASM), and in hard X-rays with INTEGRAL (17-80 keV, IBIS/ISGRI). Using data from the JEM-X monitor onboard INTEGRAL we detected 61 Type-I X-ray bursts, and confirm that the source displayed a quasi-periodic burst recurrence time of about 2.3 hours. We improve the characterization of the fuel composition, as well as the description of the typical burst durations and fluences. We estimate the average value of $\\alpha$ to be $49\\pm\\,3$. This value together with the observed burst profiles indicate a regime of a mixed He/H runaway triggered by unstable helium ignition. In addition, we report the detection of four series of double bursts, with burst recurrence times of $\\leq\\,20$ minutes. The measured recu...

  6. The periodic bursters XB1323-619 and GS1826-24: longterm evolution of the nuclear burning regime and comparison with theory

    OpenAIRE

    Balucinska-Church, M.; Reed, D.; Church, M. J.

    2007-01-01

    The majority of X-ray burst sources do not display a burst rate that increases with luminosity as expected, but this is seen in the two clocked bursters XB1323-619 and GS1826-24. We present a detailed investigation of these two sources which in the case of the first source, spans 18 years. Based on measurements of the burst rate, X-ray luminosity, the alpha-parameter and the two time constants generally present in the burst decays, we demonstrate the importance of the rp nuclear burning proce...

  7. A Soft X-Ray Spectral Episode for the Clocked Burster, GS 1826-24 as Measured by Swift and NuSTAR

    CERN Document Server

    Chenevez, J; Zand, J J M in 't; Tomsick, J A; Barret, D; Chakrabarty, D; Fürst, F; Boggs, S E; Christensen, F E; Craig, W W; Hailey, C J; Harrison, F A; Romano, P; Stern, D; Zhang, W W

    2015-01-01

    We report on NuSTAR and Swift observations of a soft state of the neutron star low-mass X-ray binary GS 1826-24, commonly known as the "clocked" burster. The transition to the soft state was recorded in 2014 June through an increase of the 2-20 keV source intensity measured by MAXI, simultaneous with a decrease of the 15-50 keV intensity measured by Swift/BAT. The episode lasted approximately two months, after which the source returned to its usual hard state. We analyze the broad-band spectrum measured by Swift/XRT and NuSTAR, and estimate the accretion rate during the soft episode to be about 13% of Eddington, within the range of previous observations. However, the best fit spectral model, adopting the double Comptonization used previously, exhibits significantly softer components. We detect seven type-I X-ray bursts, all significantly weaker (and with shorter rise and decay times) than observed previously. The burst profiles and recurrence times vary significantly, ruling out the regular bursts that are ty...

  8. A Soft X-Ray Spectral Episode for the Clocked Burster, GS 1826-24 as Measured by Swift and NuStar

    Science.gov (United States)

    Chenevez, J.; Galloway, D. K.; in 't Zand, J. J. M.; Tomsick, J. A.; Barret, D.; Chakrabarty, D.; Fürst, F.; Boggs, S. E.; Christensen, F. E.; Craig, W. W.; Hailey, C. J.; Harrison, F. A.; Romano, P.; Stern, D.; Zhang, W. W.

    2016-02-01

    We report on NuSTAR and Swift observations of a soft state of the neutron star low-mass X-ray binary GS 1826-24, commonly known as the “clocked” burster. The transition to the soft state was recorded in 2014 June through an increase of the 2-20 keV source intensity measured by MAXI, simultaneous with a decrease of the 15-50 keV intensity measured by Swift/BAT. The episode lasted approximately two months, after which the source returned to its usual hard state. We analyze the broadband spectrum measured by Swift/XRT and NuSTAR and estimate the accretion rate during the soft episode to be ≈ 13% {\\dot{m}}{{Edd}}, within the range of previous observations. However, the best-fit spectral model, adopting the double Comptonization used previously, exhibits significantly softer components. We detect seven type-I X-ray bursts, all significantly weaker (and with shorter rise and decay times) than observed previously. The burst profiles and recurrence times vary significantly, ruling out the regular bursts that are typical for this source. One burst exhibited photospheric radius expansion and we estimate the source distance as (5.7+/- 0.2) {ξ }b-1/2 kpc, where ξb parameterizes the possible anisotropy of the burst emission. The observed soft state may most likely be interpreted as a change in accretion geometry at about similar bolometric luminosity as in the hard state. The different burst behavior can therefore be attributed to this change in accretion flow geometry, but the fundamental cause and process for this effect remain unclear.

  9. A space charge model for electrophonic bursters

    CERN Document Server

    Beech, M

    1999-01-01

    The sounds accompanying electrophonic burster meteors are characteristically described as being akin to short duration ``pops'' and staccato--like ``clicks''. As a phenomenon distinct from the enduring electrophonic sounds that occasionally accompany the passage and ablation of large meteoroids in the Earth's lower atmosphere, the bursters have proved stubbornly difficult to explain. A straightforward calculation demonstrates that in contradistinction to the enduring electrophonic sounds, the electrophonic bursters are not generated as a consequence of interactions between the meteoroid ablation plasma and the Earth's geomagnetic field. Here we present a novel and hitherto unrecorded model for the generation of short--duration pulses in an observer's local electrostatic field. Our model is developed according to the generation of a strong electric field across a shock wave propagating in a plasma. In this sense, the electrophonic bursters are associated with the catastrophic disruption of large meteoroids in ...

  10. A space charge model for electrophonic bursters

    OpenAIRE

    Beech, M.; Foschini, L.

    2001-01-01

    The sounds accompanying electrophonic burster meteors are characteristically described as being akin to short duration ``pops'' and staccato--like ``clicks''. As a phenomenon distinct from the enduring electrophonic sounds that occasionally accompany the passage and ablation of large meteoroids in the Earth's lower atmosphere, the bursters have proved stubbornly difficult to explain. A straightforward calculation demonstrates that in contradistinction to the enduring electrophonic sounds, the...

  11. The evolution of Rapid Burster outbursts

    NARCIS (Netherlands)

    Guerriero, R; Fox, DW; Kommers, J; Lewin, WHG; Rutledge, R; Moore, CB; Morgan, E; Van Paradijs, J; Van der Klis, M; Bildsten, L; Dotani, T

    1999-01-01

    We describe the evolutionary progression of an outburst of the Rapid Burster. Four outbursts have been observed with the Rossi X-Ray Timing Explorer between 1996 February and 1998 May, and our observations are consistent with a standard evolution over the course of each. An outburst can be divided i

  12. Gamma-ray bursters at cosmological distances

    Science.gov (United States)

    Paczynski, B.

    1986-01-01

    It is proposed that some, perhaps most, gamma-ray bursters are at cosmological distances, like quasars, with a redshift of about 1 or 2. This proposition requires a release of supernova-like energy of about 10 to the 51st ergs within less than 1 s, making gamma-ray bursters the brightest objects known in the universe, many orders of magnitude brighter than any quasars. This power must drive a highly relativistic outflow of electron-positron plasma and radiation from the source. It is proposed that three gamma-ray bursts, all with identical spectra, detected from B1900 + 14 by Mazets, Golenetskii, and Gur'yan and reported in 1979, were all due to a single event multiply imaged by a gravitational lens. The time intervals between the successive bursts, 10 hr to 3 days, were due to differences in the light travel time for different images.

  13. Systematic Uncertainties in the Spectroscopic Measurements of Neutron-Star Masses and Radii from Thermonuclear X-ray Bursts. III. Absolute Flux Calibration

    OpenAIRE

    Guver, Tolga; Ozel, Feryal; Marshall, Herman; Psaltis, Dimitrios; Guainazzi, Matteo; Diaz-Trigo, Maria

    2015-01-01

    Many techniques for measuring neutron star radii rely on absolute flux measurements in the X-rays. As a result, one of the fundamental uncertainties in these spectroscopic measurements arises from the absolute flux calibrations of the detectors being used. Using the stable X-ray burster, GS 1826-238, and its simultaneous observations by Chandra HETG/ACIS-S and RXTE/PCA as well as by XMM-Newton EPIC-pn and RXTE/PCA, we quantify the degree of uncertainty in the flux calibration by assessing the...

  14. Possible Analogues of Cognitive Processes in the Patterns of X-ray Variability of the Rapid Burster

    CERN Document Server

    Lefebvre, V A; Lefebvre, Vladimir A.; Efremov, Yuri N.

    1999-01-01

    The bizarre patterns of rapid flashes and bursts in the X-ray source MXB 1730-335 (Rapid Burster) have been puzzling researchers for two decades; especially intriguing are its peculiarities in the time-invariant decay profiles of many type II bursts of the Rapid Burster. We have discovered that they are similar to certain regularities found in cognitive psychology, suggesting an analogy between the activity of the Rapid Burster and the cognitive activity of the human mind.

  15. A group of outbursts with exponential decays in the Rapid Burster

    Czech Academy of Sciences Publication Activity Database

    Šimon, Vojtěch

    2008-01-01

    Roč. 492, č. 1 (2008), s. 135-143. ISSN 0004-6361 R&D Projects: GA ČR GA205/05/2167 Grant ostatní: ESA(XE) ESA PRODEX INTEGRAL 90108; ESA(XE) ESA-PECS project no. 98023 Institutional research plan: CEZ:AV0Z10030501 Keywords : neutron stars * X-rays binaries * Rapid Burster (MXB 1730-335) Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.153, year: 2008

  16. Further analysis of SAS 3 observations of the rapid burster /MXB 1730-335/

    Science.gov (United States)

    Marshall, H. L.; Hoffman, J. A.; Doty, J.; Lewin, W. H. G.; Ulmer, M. P.

    1979-01-01

    SAS 3 observations of the rapid burster MXB 1730-335 are analyzed with specific reference to the rapidly repetitive type II bursts and also the type I bursts. It is found that: (1) there are two stable patterns in the recurrence of the rapidly repetitive type II bursts, designated modes I and II; (2) the range of type II burst energies is a factor of about 100 for mode I and a factor of about 10 for mode II; (3) type II burst spectra are best fitted by a blackbody of constant temperature (of the order of 18 million K), constant column density, and decreasing intensity; and (4) type I bursts are best fitted by a blackbody spectrum with decreasing temperature (approximately 24 million K during the first few seconds and 16 million K about 10 sec later). The spectral fits for both types of burst are shown to yield sizes for the emission region comparable to the radius of a neutron star. A model for the rapid burster is suggested.

  17. Contributions of gamma ray bursters and supernovae to the terrestrial background radiation field

    International Nuclear Information System (INIS)

    Supernovae and gamma ray bursters are the most energetic events known in the universe. For over three decades researchers have speculated that these events may periodically increase the terrestrial radiation field to lethal levels, perhaps periodically sterilizing the surface of every planet in the Galaxy. Indeed, calculations show that a gamma ray burster located anywhere in the Milky Way Galaxy would produce a gamma ray dose of about 200 Gy to the top of the Earth's atmosphere. However, due to attenuation by the atmosphere, dose at the surface would be considerably lower and dose at a meter underwater would be lower still. In fact, the gamma ray dose from a gamma ray burst with a gamma energy of 1053 ergs at a distance of 100 parsecs from Earth will yield a direct gamma radiation dose of about 107 Gy at the top of the earth's atmosphere, about 1 Gy at sea level, and 160 mGy at a water depth of one meter. Similar calculations have been performed for supernovae, which are two orders of magnitude less energetic. Of crucial importance in determining the radiation exposure from these events is the gamma ray spectrum because gamma ray energy determines the degree of shielding provided by the atmosphere, buildup within the atmosphere, and water as well as contributions to other interactions such as pair production and photo-neutron interactions. In addition, other supernova and gamma ray burster debris such as charged and uncharged particles that may contribute in as-yet-unknown ways to overall radiation dose from these events. Gamma ray bursts are thought to occur about once every million years in the Milky Way Galaxy and supernovae occur a few times per century. With this periodicity, we would expect about one gamma ray burst every 10-20 million years that would raise sea-level gamma radiation dose to about 10 mGy and supernovae with similar effects should occur every 20-30 million years. This suggests that, while these events may not be important from the standpoint

  18. The x-ray activity of the rapid burster in 1983

    International Nuclear Information System (INIS)

    The rapid burster became active on August 5, 1983 after a long quiescence of four years. In this active period, the source exhibited several new features. It started to emit to train of bursts which apparently resemble to type I bursts, with quasi-periodic occurrence (74-90 min) superposed on a strong persistent component of approximately 50 mCrab. From August 17 to 19 there appeared eight long bursts of trapezoidal profiles similar to those observed in August 1979, and seven exotic long bursts, each being preceded by a gradual intensity increase in a few minutes. From August 20 to the termination of the activity on August 31 were observed about 3000 rapidly repetitive short bursts characteristic to this source in its discovery in 1976. The burst interval changed day to day from 15 to 150 s. Bursts with short interval exhibited almost periodic features of 16 s, suggesting a coherent nature of the burst train. (author)

  19. VizieR Online Data Catalog: Models of thermonuclear X-ray bursters (Lampe+, 2016)

    Science.gov (United States)

    Lampe, N.; Heger, A.; Galloway, D. K.

    2016-05-01

    Using the KEPLER 1D hydrodynamics code (Woosley et al. 2004ApJS..151...75W), 464 models of thermonuclear X-ray bursters were performed across a range of accretion rates and compositions. We present the library of simulated burst profiles from this sample, and examine variations in the simulated light curve for different model conditions. We find that the recurrence time varies as a power law against accretion rate, and measure its slope while mixed H/He burning is occurring for a range of metallicities, finding the power law gradient to vary from {eta}=1.1 to 1.24. We identify the accretion rates at which mixed H/He burning stops and a transition occurs to different burning regimes. We explore how varying the accretion rate and metallicity affects burst morphology in both the rise and tail. (1 data file).

  20. Spike-adding in parabolic bursters: The role of folded-saddle canards

    Science.gov (United States)

    Desroches, Mathieu; Krupa, Martin; Rodrigues, Serafim

    2016-09-01

    The present work develops a new approach to studying parabolic bursting, and also proposes a novel four-dimensional canonical and polynomial-based parabolic burster. In addition to this new polynomial system, we also consider the conductance-based model of the Aplysia R15 neuron known as the Plant model, and a reduction of this prototypical biophysical parabolic burster to three variables, including one phase variable, namely the Baer-Rinzel-Carillo (BRC) phase model. Revisiting these models from the perspective of slow-fast dynamics reveals that the number of spikes per burst may vary upon parameter changes, however the spike-adding process occurs in an explosive fashion that involves special solutions called canards. This spike-adding canard explosion phenomenon is analysed by using tools from geometric singular perturbation theory in tandem with numerical bifurcation techniques. We find that the bifurcation structure persists across all considered systems, that is, spikes within the burst are incremented via the crossing of an excitability threshold given by a particular type of canard orbit, namely the true canard of a folded-saddle singularity. However there can be a difference in the spike-adding transitions in parameter space from one case to another, according to whether the process is continuous or discontinuous, which depends upon the geometry of the folded-saddle canard. Using these findings, we construct a new polynomial approximation of the Plant model, which retains all the key elements for parabolic bursting, including the spike-adding transitions mediated by folded-saddle canards. Finally, we briefly investigate the presence of spike-adding via canards in planar phase models of parabolic bursting, namely the theta model by Ermentrout and Kopell.

  1. Object Tracking withIphone 3Gs

    OpenAIRE

    Alin, Lars

    2010-01-01

    In June of 2007 Apple Inc. released the smartphone Iphone. It was a groundbreaking success that set a new standard for what a smartphone should be able to do. Apple has improved the Iphone every year since then and the 3Gs is the newest Iphone model. As the phones have improved, both when looking at hardware and software, the applications have improved as well. The Iphone 3Gs provides the possibility to use the camera as an application background and with that the possibility to analyze the s...

  2. A population study of type II bursts in the Rapid Burster

    CERN Document Server

    Bagnoli, T; D'Angelo, C R; Galloway, D K

    2015-01-01

    Type II bursts are thought to arise from instabilities in the accretion flow onto a neutron star in an X-ray binary. Despite having been known for almost 40 years, no model can yet satisfactorily account for all their properties. To shed light on the nature of this phenomenon and provide a reference for future theoretical work, we study the entire sample of Rossi X-ray Timing Explorer data of type II bursts from the Rapid Burster (MXB 1730-335). We find that type II bursts are Eddington-limited in flux, that a larger amount of energy goes in the bursts than in the persistent emission, that type II bursts can be as short as 0.130 s, and that the distribution of recurrence times drops abruptly below 15-18 s. We highlight the complicated feedback between type II bursts and the NS surface thermonuclear explosions known as type I bursts, and between type II bursts and the persistent emission. We review a number of models for type II bursts. While no model can reproduce all the observed burst properties and explain...

  3. Discovery of GRS 1915+105 variability patterns in the Rapid Burster

    CERN Document Server

    Bagnoli, T

    2015-01-01

    We report the discovery of two new types of variability in the neutron star low-mass X-ray binary MXB 1730-335 (the 'Rapid Burster'). In one observation in 1999, it exhibits a large-amplitude quasi-periodic oscillation with a period of about 7 min. In another observation in 2008, it exhibits two 4-min long 75 per cent deep dips 44 min apart. These two kinds of variability are very similar to the so-called $\\rho$ or 'heartbeat' variability and the $\\theta$ variability, respectively, seen in the black hole low-mass X-ray binaries GRS 1915+105 and IGR J17091-3624. This shows that these types of behavior are unrelated to a black hole nature of the accretor. Our findings also show that these kinds of behaviour need not take place at near-Eddington accretion rates. We speculate that they may rather be related to the presence of a relatively wide orbit with an orbital period in excess of a few days and about the relation between these instabilities and the type II bursts.

  4. The INTEGRAL long monitoring of persistent Ultra Compact X-ray Bursters

    CERN Document Server

    Fiocchi, M; Ubertini, P; Bird, A J; Natalucci, L; Sguera, V

    2008-01-01

    The combination of compact objects, short period variability and peculiar chemical composition of the Ultra Compact X-ray Binaries make up a very interesting laboratory to study accretion processes and thermonuclear burning on the neutron star surface. The improved large optical telescopes and more sensitive X-ray satellites have increased the number of known Ultra Compact X-ray Binaries allowing their study with unprecedented detail. We analyze the average properties common to all ultra compact Bursters observed by INTEGRAL from ~0.2keV to ~150keV. We have performed a systematic analysis of the INTEGRAL public data and Key-Program proprietary observations of a sample of the Ultra Compact X-ray Binaries. In order to study their average properties in a very broad energy band, we combined INTEGRAL with BeppoSAX and SWIFT data whenever possible. For sources not showing any significant flux variations along the INTEGRAL monitoring, we build the average spectrum by combining all available data; in the case of vari...

  5. Isolation and characterization of the human Gsα gene

    International Nuclear Information System (INIS)

    The gene for Gsα (the α subunit of the guanine nucleotide-binding protein Gs) was isolated from human genomic libraries using rat Gsα cDNA as a probe. Comparison of the nucleotide sequence of the human gene with that of the rat cDNA revealed that the human Gsα gene spans ∼20 kilobases and is composed of 13 exons and 12 introns. Genomic Southern blot analysis suggests that the human haploid genome contains a single Gsα gene. Previous reports indicated the presence of multiple species of Gsα cDNA. The structure of the human Gsα gene suggests that four types of Gsα mRNAs may be generated from a single Gsα gene by alternate use of exon 3 and/or of two 3' splice sites of intron 3, where an unusual splice junction sequence (TG) instead of the consensus (AG) is used. S1 nuclease mapping analysis of human Gsα mRNA identified multiple transcriptional initiation sites. The promoter region of the human Gsα gene has extremely high G+C content (85%). It contains 4 GC boxes, but no typical TATA or CAAT box sequence. In the 5' flanking region, there are several blocks of sequences that are similar to the sequences of the 5' flanking region of the human cKi-ras2 gene

  6. Behind the scenes of GS: preventing and curing - the GS-SE group

    CERN Multimedia

    CERN, GS-SE

    2013-01-01

    With over 3,000 CERN personnel and more than 300 visitors on the site, CERN’s infrastructure takes a real hammering every day of the year. Fortunately, the GS-SE Group is on the watch… Here, like everywhere else, nothing stays shiny and new for very long. That’s why it’s important to carry out regular, even daily, maintenance of buildings, equipment, pipes and roads which every single day see thousands of CERN personnel and visitors passing by. The team responsible for this weighty task is part of the GS-SE Group.

  7. Liquid helium centrifugal pump characteristics from 80 g/s to 1200 g/s

    CERN Document Server

    Pengo, R; Junker, S

    2010-01-01

    The large amount of data collected from three different centrifugal liquid helium pumps tested, namely with 80, 600 and 1200 g/s nominal mass flow are reviewed. The data include the analysis of the characteristic curves, their total efficiencies, their Net Positive Suction Head (NPSH) and the slip factor. The 1200 g/s pumps tested are of the full emission type, with curved blades, whilst the other pumps have straight blades. The pumps were also tested at different rotary speeds. The pumps were manufactured by Barber \\& Nichols (Denver, USA). (C) 2009 Elsevier Ltd. All rights reserved.

  8. Behind the scenes of GS: shared mobility

    CERN Multimedia

    Corinne Pralavorio

    2014-01-01

    The GS-IS team responsible for mobility is working on the problematic equation of improving transport with a growing number of people on site.   Congested car parks and sites that are far apart mean that going from one place to another at CERN often requires patience. Transport is major a concern not only for people working at the Organization, but also those in charge of the road infrastructure. The 120-hectare Meyrin site and the distance between the various sites mean that efficient modes of transport are essential. The growing number of people on site and the increasingly congested car parks have made the problem even trickier. Over the past five years, the GS-IS group has launched several initiatives to facilitate mobility on site without proportionately increasing CERN’s fleet of vehicles. Sharing is the only solution! The shuttle service has been reinforced:  since 2010, four regular routes have been set up as well as a service for operators working shifts during t...

  9. Interpretation of the burst spectra from X-ray burster MXB 1728-34 with allowance for Thomson scattering

    International Nuclear Information System (INIS)

    Spectral evolution is investigated and interpretations of the spectra are considered for a burst from the X-ray burster MXB 1728-34 detected by ASTRON. Interpretation of the burst spectra in terms of black.body radiation leads to improbably small neutron star (NS) mass M and radius R as well as distance D to the burster. Interpretation of the spectra around the burst maximum (first ≅4 s) in terms of radiation from an isothermal NS photosphere with dominating role of the Thomson scattering allows one to obtain reasonable constraints on M, R, and D (in particular, M=(1.4-2) M Sun, R=(6.5-12) km for D=6 kpk and helium photosphere). This interpretation leads to a conclusion that the photosphere expands up to several tens of kilometers in the burst maximum. The luminosity remains close to the Eddington limit during expansion and contraction; maxima of the observed flux corresponding to minima of the luminosity due to general relativity effects. The burst tail spectra are close to those of thermal bremstrahlung. Such spectra might originate in a corona around NS with mass ∼ 10 -16 MSun, radius ∼ 100 km and electron density ∼ 1019 cm-3, or in a hot layer with thickness ∼ 1-10 gxcm-2 on the photosphere

  10. Systematic Uncertainties in the Spectroscopic Measurements of Neutron-Star Masses and Radii from Thermonuclear X-ray Bursts. III. Absolute Flux Calibration

    CERN Document Server

    Guver, Tolga; Marshall, Herman; Psaltis, Dimitrios; Guainazzi, Matteo; Diaz-Trigo, Maria

    2015-01-01

    Many techniques for measuring neutron star radii rely on absolute flux measurements in the X-rays. As a result, one of the fundamental uncertainties in these spectroscopic measurements arises from the absolute flux calibrations of the detectors being used. Using the stable X-ray burster, GS 1826-238, and its simultaneous observations by Chandra HETG/ACIS-S and RXTE/PCA as well as by XMM-Newton EPIC-pn and RXTE/PCA, we quantify the degree of uncertainty in the flux calibration by assessing the differences between the measured fluxes during bursts. We find that the RXTE/PCA and the Chandra gratings measurements agree with each other within their formal uncertainties, increasing our confidence in these flux measurements. In contrast, XMM-Newton EPIC-pn measures 14.0$\\pm$0.3% less flux than the RXTE/PCA. This is consistent with the previously reported discrepancy with the flux measurements of EPIC-pn, compared to EPIC-MOS1, MOS2 and ACIS-S detectors. We also address the calibration uncertainty in the RXTE/PCA int...

  11. Behind the scenes of GS: preventing and curing - the GS-SE group (VERSION FR)

    CERN Multimedia

    CERN, GS-SE

    2013-01-01

    Avec, chaque jour, plus de 3000 Cernois sur le site et plus de 300 visiteurs, le CERN et ses infrastructures en voient des vertes et des pas mûres. Heureusement, le groupe GS-SE veille au grain… Ici comme partout ailleurs, rien ne reste jamais neuf bien longtemps. D’où l’importance d’entretenir régulièrement, pour ne pas dire « quotidiennement », les bâtiments, équipements, canalisations, routes, qui voient passer, jour après jour, des milliers de Cernois et de visiteurs. Au CERN, l’équipe en charge de cette lourde tâche fait partie du groupe GS-SE.

  12. Behind the scenes of GS: precious stones

    CERN Document Server

    Anaïs Schaeffer

    2014-01-01

    Since the laying of the foundation stone for the Synchrocyclotron in June 1954, CERN has been expanding constantly to reach a current constructed surface area of 580,000 m2 covered by some 650 buildings (just 125 of which account for 70% of this area).   This year, while CERN’s 60th anniversary is an important milestone in the Laboratory’s history, it also highlights another reality: CERN is a collection of ageing buildings, no less than 60% of which are more than 40 years old. “Of the 580,000 m2 of buildings, around 52,000 m2 are nearing the end of their useful life and 140,000 m2 are in need of extensive renovations,” says Natacha Lopez, GS-SE-PO Section Leader. “Of course, we’ve already started several renovation projects, but it's a huge challenge as we have to renovate buildings constructed in the 1960s and 70s while complying with modern standards, which are very different from those that were in force when they were bu...

  13. Behind the scenes of GS: Open Sesame!

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    The section in the GS-IS Group responsible for running the CERN Stores processes hundreds of orders per day, checks the quality of each and every product and is responsible for warehousing thousands of items available in a richly-stocked catalogue.   Sprawling across several buildings (73, 128 and 129), a total surface area of 3640 m2, and providing 28,300 cubic metres of storage space, the equivalent of 330 semi-trailers, the CERN stores are a veritable treasure trove! The products on offer range from office stationery and computer hardware to fire extinguishers, cables, steel and chemicals. The new (above) and old storage system for the CERN stores. The CERN Stores catalogue contains nearly 48,000 referenced items, 12,000 of which are available on site. Goods arrive at either Building 194 (Meyrin) or Building 904 (Prévessin) and are then forwarded to the central stores in Building 73. “Every item that arrives here undergoes a strict quality control,” says M...

  14. Behind the scenes of GS: Sweet dreams!

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    The CERN Hostel is located in the heart of the Meyrin site and provides the Organization’s many users with a comfortable and peaceful place to stay. The services offered by the Hostel, which is managed by the GS-IS Group, have seen many changes over the years.   The first CERN Hostel (Building 38) was built in 1982 in response to an increasing demand for accommodation, which until then had been met by a handful of converted bedrooms in Building 5 and, since 1974, by 81 additional bedrooms made available to CERN through an agreement with the Foyer Schumann in Saint-Genis-Pouilly. Let’s not even mention the fact that the barracks now occupied by the CERN clubs used to be dormitories… The Hostel was gradually extended, particularly in 1995 with the construction of Building 39, and then in 2007, when Building 41 was added, resulting in a total of 413 rooms (495 beds) today. In addition, a new agreement now gives users access to 151 low-cost rooms at the Foyer Schuma...

  15. User-Defined Features in GS-ZDDS%GS-ZDDS中的自定义特征

    Institute of Scientific and Technical Information of China (English)

    罗尚虎; 董金祥

    2001-01-01

    An approach to represent user-defined features is proposed and implemented in a 2D parametric sketching package GS-ZDDS. It provides a mechanism for information sharing between GS-ZDDS and user's professional design program. It makes the design process more efficient and enables the reusing of designed part. By using user-defined features, the amount of programming needed to build user's professional design system can be reduced significantly, moreover, the buildup system can be extended easily.%在自主开发的二维参数化绘图软件中,提出了一种用于与用户专业设计程序相集成的自定义特征的概念,实现了设计计算过程与结构设计过程的信息共享和局部并行,提高了设计效率,体现了设计构件重用的思想,并且大大减小了用户专用的设计系统开发的工作量.系统具有良好的可扩展性.

  16. Regulation of PDH, GS and insulin signalling in skeletal muscle

    DEFF Research Database (Denmark)

    Biensø, Rasmus Sjørup

    The aims of the present thesis were to investigate 1) The impact of physical inactivity on insulinstimulated Akt, TBC1D4 and GS regulation in human skeletal muscle, 2) The impact of exercise training on glucose-mediated regulation of PDH and GS in skeletal muscle in elderly men, 3) The impact of...... inflammation on resting and exercise-induced PDH regulation in human skeletal muscle and 4) The effect of IL-6 on PDH regulation in mouse skeletal muscle. Study I demonstrated that bed rest–induced insulin resistance was associated with reduced insulinstimulated GS activity and Akt signaling as well as...... glucose to the level seen when exercise was performed before bed rest. Study II demonstrated that exercise training-improved glucose regulation in elderly healthy subjects was associated with increased HKII, GLUT4, Akt2, PDK2, GS and PDH-E1α protein content. Moreover, exercise training resulted in an...

  17. Cardiac Effects of Attenuating Gsα - Dependent Signaling.

    Directory of Open Access Journals (Sweden)

    Marcus R Streit

    Full Text Available Inhibition of β-adrenergic signalling plays a key role in treatment of heart failure. Gsα is essential for β-adrenergic signal transduction. In order to reduce side-effects of beta-adrenergic inhibition diminishing β-adrenergic signalling in the heart at the level of Gsα is a promising option.We analyzed the influence of Gsα on regulation of myocardial function and development of cardiac hypertrophy, using a transgenic mouse model (C57BL6/J mice overexpressing a dominant negative Gsα-mutant under control of the α-MHC-promotor. Cardiac phenotype was characterized in vivo and in vitro and under acute and chronic β-adrenergic stimulation. At rest, Gsα-DN-mice showed bradycardia (602 ± 13 vs. 660 ± 17 bpm, p<0.05 and decreased dp/dtmax (5037 ± 546- vs. 6835 ± 505 mmHg/s, p = 0.02. No significant differences were found regarding ejection fraction, heart weight and cardiomyocyte size. β-blockade by propranolol revealed no baseline differences of hemodynamic parameters between wildtype and Gsα-DN-mice. Acute adrenergic stimulation resulted in decreased β-adrenergic responsiveness in Gsα-DN-mice. Under chronic adrenergic stimulation, wildtype mice developed myocardial hypertrophy associated with increase of LV/BW-ratio by 23% (4.4 ± 0.2 vs. 3.5 ± 0.1 mg/g, p<0.01 and cardiac myocyte size by 24% (14927 ± 442 px vs. 12013 ± 583 px, p<0.001. In contrast, both parameters were unchanged in Gsα-DN-mice after chronic isoproterenol stimulation.Overexpression of a dominant negative mutant of Gsα leads to decreased β-adrenergic responsiveness and is protective against isoproterenol-induced hypertrophy. Thus, Gsα-DN-mice provide novel insights into β-adrenergic signal transduction and its modulation in myocardial overload and failure.

  18. Broadband observations of the X-ray burster 4U 1705-44 with BeppoSAX

    CERN Document Server

    Piraino, S; Mueck, B; Kaaret, P; Di Salvo, T; D'Ai, A; Iaria, R; Egron, E

    2016-01-01

    4U 1705-44 is one of the most-studied type I X-ray burster and Atoll sources. This source represents a perfect candidate to test different models proposed to self-consistently track the physical changes occurring between different spectral states because it shows clear spectral state transitions. The broadband coverage, the sensitivity and energy resolution of the BeppoSAX satellite offers the opportunity to disentangle the components that form the total X-ray spectrum and to study their changes according to the spectral state. Using two BeppoSAX observations carried out in August and October 2000, respectively, for a total effective exposure time of about 100 ks, we study the spectral evolution of the source from a soft to hard state. Energy spectra are selected according to the source position in the color-color diagram (CCD) Results. We succeeded in modeling the spectra of the source using a physical self-consistent scenario for both the island and banana branches (the double Comptonization scenario). The ...

  19. Behinds the scenes of GS: a DSO like no other

    CERN Multimedia

    Antonella Del Rosso

    2014-01-01

    At CERN, Departmental Safety Officers (DSOs) are responsible for making the members of their department aware of safety issues. They’re our first point of call every time a problem arises relating to environmental matters or the safety of people and installations. In GS, this role is even more crucial as the Department’s activities are scattered across the Laboratory and affect everyone.   As we have pointed out in our article series "Behind the scenes of GS”, the GS Department is responsible for the construction, renovation and maintenance of buildings and related technical infrastructures. The latter include heating and toilet facilities; detection and alarm systems; the management of the hotels, stores, stocks, shuttle services and mail; and the development of technical and administrative databases. The activities of the Medical Service and the Fire and Rescue Service also come under the umbrella of GS, as do the many other daily activities that are pa...

  20. A soft X-ray spectral episode for the Clocked Burster, GS 1826-24 as measured by Swift and NuSTAR

    DEFF Research Database (Denmark)

    Chenevez, J.; Galloway, D.K.; in ’t Zand, J.J.M.;

    2016-01-01

    % mEdd within the range of previous observations. However, the best-fit spectral model, adopting the double Comptonization used previously, exhibits significantly softer components. We detect seven type-I X-ray bursts, all significantly weaker (and with shorter rise and decay times) than observed...

  1. Broadband observations of the X-ray burster 4U1705-44 with BeppoSAX

    Science.gov (United States)

    Piraino, S.; Santangelo, A.; Mück, B.; Kaaret, P.; Di Salvo, T.; D'Aì, A.; Iaria, R.; Egron, E.

    2016-06-01

    Context. 4U1705-44 is one of the most-studied type I X-ray burster and Atoll sources. This source represents a perfect candidate to test different models proposed to self-consistently track the physical changes occurring between different spectral states because it shows clear spectral state transitions. Aims: The broadband coverage, the sensitivity and energy resolution of the BeppoSAX satellite offers the opportunity to disentangle the components that form the total X-ray spectrum and to study their changes according to the spectral state. Methods: Using two BeppoSAX observations carried out in August and October 2000, respectively, for a total effective exposure time of ~100 ks, we study the spectral evolution of the source from a soft to hard state. Energy spectra are selected according to the source position in the color-color diagram (CCD). Results: We succeeded in modeling the spectra of the source using a physical self-consistent scenario for both the island and banana branches (the double Comptonization scenario). The components observed are the soft Comptonization and hard Comptonization, the blackbody, and a reflection component with a broad iron line. When the source moves from the banana state to the island state, the parameters of the two Comptonization components change significantly and the blackbody component becomes too weak to be detected. Conclusions: We interpret the soft Comptonization component as emission from the hot plasma surrounding the neutron star, hard Comptonization as emission from the disk region, and the blackbody component as emission from the inner accretion disk. The broad feature in the iron line region is compatible with reflection from the inner accretion disk.

  2. A Narrative Approach to Exploring TGfU-GS

    Science.gov (United States)

    Stolz, Steven A.; Pill, Shane

    2016-01-01

    This paper uses a narrative approach in the form of a fictional dialogue between a physical education teacher educator (PETE) and an enquiring physical education teacher (EPET) in order to both contextualise the problem posed by Almond, that Teaching Games for Understanding-Game Sense (TGfU-GS) has been better accepted in academia than in the…

  3. Characterization of a glutamine synthetase gene DvGS2 from Dunaliella viridis and biochemical identification of DvGS2-transgenic Arabidopsis thaliana.

    Science.gov (United States)

    Zhu, Chenguang; Fan, Qianlan; Wang, Wei; Shen, Chunlei; Meng, Xiangzong; Tang, Yuanping; Mei, Bing; Xu, Zhengkai; Song, Rentao

    2014-02-25

    The salt-tolerant green alga Dunaliella has remarkable capability to survive in some extreme environments such as nitrogen starvation, which makes Dunaliella be a proper model for mining novel genes on nitrogen uptake or assimilation. In this study, a glutamine synthetase (GS) gene DvGS2 with amino acid identity of 72% to other homologous GS proteins, was isolated and characterized from Dunaliella viridis. Phylogenetic comparison with other GSs revealed that DvGS2 occupied an independent phylogenetic position. Expressional analysis in D. viridis cells under nitrogen starvation confirmed that DvGS2 increased its mRNA level in 12h. Subcellular localization study and functional analysis in a GS-deficient Escherichia coli mutant proved that DvGS2 was a chloroplastic and functional GS enzyme. In order to investigate the potential application of DvGS2 in higher plants, the transgenic studies of DvGS2 in Arabidopsis thaliana were carried out. Results showed that the transgenic lines expressed the DvGS2 gene and demonstrated obviously enhanced root length (29%), fresh weight (40%-48% at two concentrations of nitrate supplies), stem length (21%), leaf size (39%) and silique number (44%) in contrast with the wild-type Arabidopsis. Furthermore, the transgenic lines had higher total nitrogen content (35%-43%), total GS activity (39%-45%) and soluble protein concentration (23%-24%) than the wild type. These results indicated that the overexpression of DvGS2 in A. thaliana resulted in higher biomass and the improvement of the host's nitrogen use efficiency. PMID:24334123

  4. Behind the scenes of GS: an eye for accuracy

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    The CERN sites are constantly evolving… with new buildings, extensions, redevelopment and renovation. The team in charge of geographical information in the GS Department measures and records even the smallest changes so that it can provide everyone at CERN with the most up-to-date plans possible.   The “Patrimony” team in the GS-SE-DOP Section is responsible for geographical information and has the job of updating all of the information relating to CERN's property, including the land made available to the Organization by Switzerland (110 hectares) and France (516 hectares), green spaces, buildings, underground structures, underground networks (gas, water, electricity, etc. – for which the "Patrimony” team works closely with numerous other groups and departments), car parks, roads… and much more. “Our team consists of seven surveyors and GIS specialists, who identify, define, measure, catalogue and pinpoi...

  5. Behind the scenes of GS: the impact of IMPACT

    CERN Multimedia

    Katarina Anthony

    2014-01-01

    Carrying out a job at CERN can be a complicated task, with coordinators reaching across departments to manage personnel, ensure safety and minimise the impact of their activities on the rest of the Laboratory.  To help coordinators with this tough task, the GS Department developed IMPACT, the platform that, since 2011, has unified CERN's major experiment, accelerator and injector coordination tools.   When planning interventions both large and small, IMPACT (the Intervention Management Planning and Coordination Tool) is the go-to gizmo on every CERN coordinator's tool belt. "IMPACT is a central repository of activity requests that standardises the way work is declared at CERN," says Benoit Daudin, GS-AIS-PM Section Leader. "If you need to intervene in any of CERN's major facilities, you need to declare this work on IMPACT. The tool will analyse the job and see whose approval is required. This could simply b...

  6. Evaluation of Two Guralp Preamplifiers for GS21 Seismometer Application.

    Energy Technology Data Exchange (ETDEWEB)

    Merchant, Bion J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Slad, George William [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-08-01

    Sandia National Laboratories has tested and evaluated two Guralp preamplifiers for use with a GS21 seismometer application. The two preamplifiers have a gain factor of 61.39. The purpose of the preamplifier evaluation was to determine a measured gain factor, transfer function, total harmonic distortion, self-noise, application passband, dynamic range, seismometer calibration pass-through, and to comment on any issues encountered during the evaluation. The test results included in this report were in response to static, tonal, and dynamic input signals. The Guralp GS21 preamplifiers are being evaluated for potential use in the International Monitoring System (IMS) of the Comprehensive Nuclear Test-Ban-Treaty Organization (CTBTO). Test methodologies used were based on IEEE Standards 1057 for Digitizing Waveform Recorders and 1241 for Analog to Digital Converters

  7. Test Report : GS battery, EPC power HES RESCU.

    Energy Technology Data Exchange (ETDEWEB)

    Rose, David Martin; Schenkman, Benjamin L.; Borneo, Daniel R.

    2013-10-01

    The Department of Energy Office of Electricity (DOE/OE), Sandia National Laboratories (SNL) and the Base Camp Integration Lab (BCIL) partnered together to incorporate an energy storage system into a microgrid configured Forward Operating Base to reduce the fossil fuel consumption and to ultimately save lives. Energy storage vendors will be sending their systems to SNL Energy Storage Test Pad (ESTP) for functional testing and then to the BCIL for performance evaluation. The technologies that will be tested are electro-chemical energy storage systems comprising of lead acid, lithium-ion or zinc-bromide. GS Battery and EPC Power have developed an energy storage system that utilizes zinc-bromide flow batteries to save fuel on a military microgrid. This report contains the testing results and some limited analysis of performance of the GS Battery, EPC Power HES RESCU.

  8. Probing the MSP prenatal stage: the optical identification of the X-ray burster EXO 1745-248 in Terzan 5

    CERN Document Server

    Ferraro, F R; Lanzoni, B; Cadelano, M; Massari, D; Dalessandro, E; Mucciarelli, A; -,

    2015-01-01

    We report on the optical identification of the neutron star burster EXO 1745-248 in Terzan 5. The identification was performed by exploiting HST/ACS images acquired in Director's Discretionary Time shortly after (approximately 1 month) the Swift detection of the X-ray burst. The comparison between these images and previous archival data revealed the presence of a star that currently brightened by ~3 magnitudes, consistent with expectations during an X-ray outburst. The centroid of this object well agrees with the position, in the archival images, of a star located in the Turn-Off/Sub Giant Branch region of Terzan 5. This supports the scenario that the companion should has recently filled its Roche Lobe. Such a system represents the pre-natal stage of a millisecond pulsar, an evolutionary phase during which heavy mass accretion on the compact object occurs, thus producing X-ray outbursts and re-accelerating the neutron star.

  9. Behind the scenes of GS: nothing left to chance

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    The AS (Alarm Systems) Section in the GS-ASE Group is, as its name suggests, in charge of the various alarm systems spread across CERN’s many sites. Its mission? To install, manage and maintain more than 26,000 alarms of all types located both above ground and in the tunnels.   Detection Among these systems, the best known are of course the heat and/or smoke detectors, which quickly raise the alarm in the event of a fire. CERN has 8500 of these devices in total. In combination with these, evacuation alarms are also found all over the Laboratory, including some 1800 break glass call points for 2000 sirens. In the LHC tunnel, the evacuation alarms are connected to 200 Oxygen Deficiency Hazard (ODH) sensors, but this is not the only way of triggering an alarm. “The Fire Brigade permanently monitors the evolution of safety conditions in the LHC tunnels,” says Henrik Nissen, who is responsible for “Alarm Transmission” in the GS-ASE-AS Section. &...

  10. Behind the scenes of GS: preventing and curing

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    With about 10,000 persons on the site every day, CERN’s infrastructure takes a real hammering every day of the year. Fortunately, the GS-SE Group is on the watch…   Here, like everywhere else, nothing stays shiny and new for very long. That’s why it’s important to carry out regular, even daily, maintenance of buildings, equipment, pipes and roads which every single day see thousands of CERN personnel and visitors passing by. The team responsible for this weighty task is part of the GS-SE Group. Suspicious steam All CERN’s buildings are heated by two heating plants (see the article in Bulletin 13-14/2011) via the underground hot-water network. Seen from below the ground, this is a labyrinth of pipes stretching over 30 km (including both the Meyrin and Prévessin sites). And with some of these pipes now over 40 years old, it goes without saying that leaks are a common hazard. “At the end of 2013, we had to deal with a big l...

  11. One-pot solvothermal synthesis of ZnSe·xN2H4/GS and ZnSe/N-GS and enhanced visible-light photocatalysis.

    Science.gov (United States)

    Liu, Bitao; Tian, Liangliang; Wang, Yuhua

    2013-09-11

    Doped-graphene has attracted considerable attention in many fields because doping element can alter the electrical properties of graphene. In this paper, we synthesized ZnSe·xN2H4/graphene (ZnSe·xN2H4/GS) and ZnSe/nitrogen-doped graphene (ZnSe/N-GS) nanocomposites with p-n junctions via one-pot solvothermal process. The structure, morphologies and catalytic performance of the ZnSe·xN2H4/GS and ZnSe/N-GS are characterized by X-ray diffraction pattern (XRD), field emission scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy (RS), X-ray photoelectron spectroscopy (XPS), and cathodoluminescence spectrum (CL), respectively. Our experiments show that the as-prepared nanocomposites ZnSe·xN2H4/GS and ZnSe/N-GS exhibit remarkably enhanced photocatalytic activities for methylene blue (MB) dye under visible light irradiation. Even importantly, ZnSe/N-GS would make this degradation process more effective. Overall, this facile and catalyst-free synthesize method in this work could provide new insights into the fabrication of other composites based on doped graphene with high performance photocatalysts, which show their potential applications in producing of hydrogen through water splitting, environmental protection issues. PMID:23945131

  12. G-Protein α-Subunit Gsα Is Required for Craniofacial Morphogenesis

    OpenAIRE

    Run Lei; Ke Zhang; Yanxia Wei; Min Chen; Weinstein, Lee S.; Yang Hong; Minyan Zhu; Hongchang Li; Huashun Li

    2016-01-01

    The heterotrimeric G protein subunit Gsα couples receptors to activate adenylyl cyclase and is required for the intracellular cAMP response and protein kinase A (PKA) activation. Gsα is ubiquitously expressed in many cell types; however, the role of Gsα in neural crest cells (NCCs) remains unclear. Here we report that NCCs-specific Gsα knockout mice die within hours after birth and exhibit dramatic craniofacial malformations, including hypoplastic maxilla and mandible, cleft palate and cranio...

  13. Ground Source Integrated Heat Pump (GS-IHP) Development

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, V. D. [ORNL; Rice, K. [ORNL; Murphy, R. [ORNL; Munk, J. [ORNL; Ally, Moonis [ORNL; Shen, Bo [ORNL; Craddick, William [ORNL; Hearn, Shawn A. [ClimateMaster, Inc.

    2013-05-24

    Between October 2008 and May 2013 ORNL and ClimateMaster, Inc. (CM) engaged in a Cooperative Research and Development Agreement (CRADA) to develop a groundsource integrated heat pump (GS-IHP) system for the US residential market. A initial prototype was designed and fabricated, lab-tested, and modeled in TRNSYS (SOLAR Energy Laboratory, et al, 2010) to predict annual performance relative to 1) a baseline suite of equipment meeting minimum efficiency standards in effect in 2006 (combination of air-source heat pump (ASHP) and resistance water heater) and 2) a state-of-the-art (SOA) two-capacity ground-source heat pump with desuperheater water heater (WH) option (GSHPwDS). Predicted total annual energy savings, while providing space conditioning and water heating for a 2600 ft{sup 2} (242 m{sup 2}) house at 5 U.S. locations, ranged from 52 to 59%, averaging 55%, relative to the minimum efficiency suite. Predicted energy use for water heating was reduced 68 to 78% relative to resistance WH. Predicted total annual savings for the GSHPwDS relative to the same baseline averaged 22.6% with water heating energy use reduced by 10 to 30% from desuperheater contributions. The 1st generation (or alpha) prototype design for the GS-IHP was finalized in 2010 and field test samples were fabricated for testing by CM and by ORNL. Two of the alpha units were installed in 3700 ft{sup 2} (345 m{sup 2}) houses at the ZEBRAlliance site in Oak Ridge and field tested during 2011. Based on the steady-state performance demonstrated by the GS-IHPs it was projected that it would achieve >52% energy savings relative to the minimum efficiency suite at this specific site. A number of operational issues with the alpha units were identified indicating design changes needed to the system before market introduction could be accomplished. These were communicated to CM throughout the field test period. Based on the alpha unit test results and the diagnostic information coming from the field test

  14. G-Protein α-Subunit Gsα Is Required for Craniofacial Morphogenesis.

    Science.gov (United States)

    Lei, Run; Zhang, Ke; Wei, Yanxia; Chen, Min; Weinstein, Lee S; Hong, Yang; Zhu, Minyan; Li, Hongchang; Li, Huashun

    2016-01-01

    The heterotrimeric G protein subunit Gsα couples receptors to activate adenylyl cyclase and is required for the intracellular cAMP response and protein kinase A (PKA) activation. Gsα is ubiquitously expressed in many cell types; however, the role of Gsα in neural crest cells (NCCs) remains unclear. Here we report that NCCs-specific Gsα knockout mice die within hours after birth and exhibit dramatic craniofacial malformations, including hypoplastic maxilla and mandible, cleft palate and craniofacial skeleton defects. Histological and anatomical analysis reveal that the cleft palate in Gsα knockout mice is a secondary defect resulting from craniofacial skeleton deficiencies. In Gsα knockout mice, the morphologies of NCCs-derived cranial nerves are normal, but the development of dorsal root and sympathetic ganglia are impaired. Furthermore, loss of Gsα in NCCs does not affect cranial NCCs migration or cell proliferation, but significantly accelerate osteochondrogenic differentiation. Taken together, our study suggests that Gsα is required for neural crest cells-derived craniofacial development. PMID:26859889

  15. G-Protein α-Subunit Gsα Is Required for Craniofacial Morphogenesis.

    Directory of Open Access Journals (Sweden)

    Run Lei

    Full Text Available The heterotrimeric G protein subunit Gsα couples receptors to activate adenylyl cyclase and is required for the intracellular cAMP response and protein kinase A (PKA activation. Gsα is ubiquitously expressed in many cell types; however, the role of Gsα in neural crest cells (NCCs remains unclear. Here we report that NCCs-specific Gsα knockout mice die within hours after birth and exhibit dramatic craniofacial malformations, including hypoplastic maxilla and mandible, cleft palate and craniofacial skeleton defects. Histological and anatomical analysis reveal that the cleft palate in Gsα knockout mice is a secondary defect resulting from craniofacial skeleton deficiencies. In Gsα knockout mice, the morphologies of NCCs-derived cranial nerves are normal, but the development of dorsal root and sympathetic ganglia are impaired. Furthermore, loss of Gsα in NCCs does not affect cranial NCCs migration or cell proliferation, but significantly accelerate osteochondrogenic differentiation. Taken together, our study suggests that Gsα is required for neural crest cells-derived craniofacial development.

  16. GS6, A Member of the GRAS Gene Family, Negatively Regulates Grain Size in Rice

    Institute of Scientific and Technical Information of China (English)

    Lianjun Sun; Xiaojiao Li; Yongcai Fu; Zuofeng Zhu; Lubin Tan; Fengxia Liu; Xianyou Sun; Xuewen Sun; Chuanqing Sun

    2013-01-01

    Grain size is an important yield-related trait in rice. Intensive artificial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with their wild relatives. However, the molecular genetic control of rice grain size is still not well characterized. Here, we report the identification and cloning of Grain Size 6 (GS6), which plays an important role in reducing grain size in rice. A premature stop at the þ348 position in the coding sequence (CDS) of GS6 increased grain width and weight significantly. Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6 alleles. Most japonica varieties (95%) harbor the Type I haplotype, and 62.9%of indica varieties harbor the Type II haplotype. Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes. Further investigation of genetic diversity and the evolutionary mechanisms of GS6 showed that the GS6 gene was strongly selected in japonica cultivars. In addition, a “ggc” repeat region identified in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice. Knowledge of the function of GS6 might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants, and could facilitate the genetic improvement of rice yield.

  17. Behind the scenes of GS: keeping CERN clean

    CERN Multimedia

    Antonella Del Rosso

    2014-01-01

    More than 350,000 square metres to be maintained, two different cleaning companies due to CERN’s sites being in two different countries, underground installations where cleanliness is a question of safety… the CERN team in charge of supervising cleaning services overcomes all these challenges to ensure that our workplace remains clean and pleasant every day.   Ensuring the cleanliness of sites containing a huge variety of buildings is an enormous challenge for the two experts in the GS Department in charge of cleaning services. Alain Bertrand has been at CERN for 29 years and knows all there is to know about the Organization’s buildings and what they are used for, the condition of their toilet facilities and, of course, the location of the border between France and Switzerland which, in terms of cleaning services, denotes the transition point between the two companies used. David Chameaux has been doing the same job as Alain for two years, working with him to ensu...

  18. Behind the scenes of GS: security affects us all

    CERN Multimedia

    Antonella Del Rosso

    2014-01-01

    A CERN manager, supported by two outside companies, manages the three entities in the GS Department that are in charge of various aspects of security. In total, about 80 people, 300 cameras, a surveillance centre (CSA) and 22 kilometres of fences are responsible for ensuring that the Organization is secure. But in spite of these significant resources, the best strategy for maintaining a good level of security at CERN is the active support of its users.   CERN’s security service covers three main areas: access control, comprising the security guards and a patrol service that ensures the site is secure and monitors compliance with traffic and parking rules; registration; and locks and keys. The 22 guards on the day shift and the seven on the night shift, who constantly monitor access, are spread across the two main sites and the four experiment sites. “The guards’ main job is to check that only those who are authorised to do so enter the site,” explains...

  19. Behind the scenes of GS: Service orientation CERN-wide

    CERN Multimedia

    Corinne Pralavorio

    2014-01-01

    A far-reaching project to improve the management of services was initiated in 2010, through the implementation of standard methods and tools to improve the efficiency of CERN services continuously.   Everyone at CERN now knows the telephone number 77777, the e-mail address service-desk@cern.ch and the CERN Service Portal. Behind all this lies a one-stop-shop where a team of operators is ready to answer your call as swiftly as possible and help you send that over-sized parcel to Patagonia or repair those faulty blinds (see Bulletin article 44-45/2013). But that’s not all, as Reinoud Martens, head of the Service Management Support Group in the GS Department, explains: “As far as the service management project is concerned, receiving and assigning incoming calls is just the tip of the iceberg! More generally, the aim of this massive project, launched in 2010 in close collaboration with the IT Department, is to enhance service management and implement standardised met...

  20. Summoning earth and fire: The energy development implications of Grameen Shakti (GS) in Bangladesh

    International Nuclear Information System (INIS)

    Since its establishment in 1996, the nonprofit company Grameen Shakti (GS) has installed almost half a million solar home systems (SHS), 132,000 cookstoves, and 13,300 biogas plants among 3.1 million beneficiaries. They plan to ramp up their expansion so that by 2015, more than 1.5 million SHS are in place along with 100,000 biogas units and 5 million improved cookstoves. This article describes GS's current activities, the contours of its programs, and likely reasons for its success. It also explores the remaining challenges facing GS and distils common lessons for other energy development assistance projects and programs around the world. After detailing research methods consisting primarily of research interviews and site visits, the article briefly explores the history of GS and summarizes its three most prominent programs. The article then identifies six distinct benefits to their programs-expansion of energy access, less deforestation and fewer greenhouse gas emissions, price savings, direct employment and income generation, improved public health, and better technology-before discussing challenges related to staff retention and organizational growth, living standards, technical obstacles, affordability, tension with other energy programs, political constraints, and awareness and cultural values. -- Highlights: → Grameen Shakti (GS) has installed a large number of solar home systems (SHS), cookstoves, and biogas plants. → GS programs have brought a wide array of social and economic benefits. → GS programs face internal and external challenges if they are to remain successful.

  1. Transgenic poplar expressing the pine GS1a show alterations in nitrogen homeostasis during drought.

    Science.gov (United States)

    Molina-Rueda, Juan Jesús; Kirby, Edward G

    2015-09-01

    Transgenic hybrid poplars engineered to express ectopically the heterologous pine cytosolic GS1a display a number of significant pleiotropic phenotypes including enhanced growth, enhanced nitrogen use efficiency, and resistance to drought stress. The present study was undertaken in order to assess mechanisms whereby ectopic expression of pine GS1a in transgenic poplars results in enhanced agronomic phenotypes. Microarray analysis using the Agilent Populus whole genome array has allowed identification of genes differentially expressed between wild type (WT) and GS transgenics in four tissues (sink leaves, source leaves, stems, and roots) under three growth conditions (well-watered, drought, and recovery). Analysis revealed that differentially expressed genes in functional categories related to nitrogen metabolism show a trend of significant down-regulation in GS poplars compared to the WT, including genes encoding nitrate and nitrite reductases. The down-regulation of these genes was verified using qPCR, and downstream effects were further tested using NR activity assays. Results suggest that higher glutamine levels in GS transgenics regulate nitrate uptake and reduction. Transcript levels of nitrogen-related genes in leaves, including GS/GOGAT cycle enzymes, aspartate aminotransferase, GABA shunt enzymes, photorespiration enzymes, asparagine synthetase, phenylalanine ammonia lyase, isocitrate dehydrogenase, and PII, were also assessed using qPCR revealing significant differences between GS poplars and the WT. Moreover, metabolites related to these differentially expressed genes showed alterations in levels, including higher levels of GABA, hydroxyproline, and putrescine in the GS transgenic. These alterations in nitrogen homeostasis offer insights into mechanisms accounting for drought tolerance observed in GS poplars. PMID:26113157

  2. Weighted 3D GS Algorithm for Image-Qquality Improvement of Multi-Plane Holographic Display

    Institute of Scientific and Technical Information of China (English)

    李芳; 毕勇; 王皓; 孙敏远; 孔新新

    2012-01-01

    Theoretically,three-dimensional (3D) GS algorithm can realize 3D displays; however,correlation of the output image is restricted because of the interaction among multiple planes,thus failing to meet the image-quality requirements in practical applications.We introduce the weight factors and propose the weighted 3D GS algorithm,which can realize selective control of the correlation of multi-plane display based on the traditional 3D GS algorithm.Improvement in image quality is accomplished by the selection of appropriate weight factors.

  3. Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety.

    Science.gov (United States)

    Lee, Jong-Yeol; Beom, Hye-Rang; Altenbach, Susan B; Lim, Sun-Hyung; Kim, Yeong-Tae; Kang, Chon-Sik; Yoon, Ung-Han; Gupta, Ravi; Kim, Sun-Tae; Ahn, Sang-Nag; Kim, Young-Mi

    2016-05-01

    Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour. PMID:26882917

  4. GS-5806 Inhibits Pre- to Postfusion Conformational Changes of the Respiratory Syncytial Virus Fusion Protein

    OpenAIRE

    Samuel, Dharmaraj; Xing, Weimei; Niedziela-Majka, Anita; Wong, Jinny S; Hung, Magdeleine; Brendza, Katherine M.; Perron, Michel; Jordan, Robert; Sperandio, David; Liu, Xiaohong; Mackman, Richard; Sakowicz, Roman

    2015-01-01

    GS-5806 is a small-molecule inhibitor of human respiratory syncytial virus fusion protein-mediated viral entry. During viral entry, the fusion protein undergoes major conformational changes, resulting in fusion of the viral envelope with the host cell membrane. This process is reproduced in vitro using a purified, truncated respiratory syncytial virus (RSV) fusion protein. GS-5806 blocked these conformational changes, suggesting a possible mechanism for antiviral activity.

  5. Characterization of a glutamine synthetase gene DvGS1 from Dunaliella viridis and investigation of the impact on expression of DvGS1 in transgenic Arabidopsis thaliana.

    Science.gov (United States)

    Zhu, Chenguang; Fan, Qianlan; Wang, Wei; Shen, Chunlei; Wang, Peipei; Meng, Xiangzong; Tang, Yuanping; Mei, Bing; Xu, Zhengkai; Song, Rentao

    2014-01-01

    A novel glutamine synthetase (GS) gene DvGS1 showing highest amino acid sequence identity of 78 % with the other homologous GS proteins from green algae, was isolated and characterized from Dunaliella viridis. Phylogenetic analysis revealed that DvGS1 occupied an independent phylogenetic position which was different with the GSs from higher plants, animals and microbes. Functional complement in E. coli mutant confirmed that the DvGS1 encoded functional GS enzyme. Real-time PCR analysis of DvGS1 in D. viridis cells under nitrogen starvation revealed that the mRNA level of DvGS1 was positively up-regulated in 12 h. The DvGS1 levels at the points of 12 and 24 h were separately twofold and fourfold of the level before nitrogen starvation. In order to investigate the potential application of DvGS1 in higher plants, the transgenic study of DvGS1 in Arabidopsis thaliana was carried out. Phenotype identification demonstrated that all three transgenic lines of T3 generation showed obviously enhanced root length (26 %), fresh weight (22-46 % at two concentrations of nitrate supplies), stem length (26 %), leaf size (29 %) and silique number (30 %) compared with the wild-type Arabidopsis. Biochemical analysis confirmed that all three transgenic lines had higher total nitrogen content, soluble protein concentration, total amino acid content and the leaf GS activity than the wild type plants. The free NH4 (+) and NO3 (-) concentration in fresh leaves of three transgenic lines were reduced by 17-26 % and 14-15 % separately (at two concentrations of nitrate supplies) compared with those of the wild types. All the results indicated that over-expression of DvGS1 in Arabidopsis significantly results in the improvement of growth phenotype and the host's nitrogen use efficiency. PMID:24307252

  6. A tarantula spider toxin, GsMTx4, reduces mechanical and neuropathic pain.

    Science.gov (United States)

    Park, Seung Pyo; Kim, Byung Moon; Koo, Jae Yeon; Cho, Hawon; Lee, Chang Hoon; Kim, Misook; Na, Heung Sik; Oh, Uhtaek

    2008-07-01

    Mechanosensitive channels mediate various physiological functions including somatic sensation or pain. One of the peptide toxins isolated from the venom of the Chilean rose tarantula spider (Grammostola spatulata), mechanotoxin 4 (GsMTx4) is known to block stretch-activated cation channels. Since mechanosensitive channels in sensory neurons are thought to be molecular sensors for mechanotransduction, i.e., for touch, pressure, proprioception, and pain, we considered that the venom might block some types of mechanical pain. In order to prepare sufficiently large amounts of GsMTx4 for in vivo nociceptive behavioral tests, we constructed recombinant peptide of GsMTx4. Because the amino-acid sequence of the toxin, but not the nucleotide sequence, is known, we back-translated its amino-acid sequence to nucleotide sequence of yeast codons, constructed a template DNA, subcloned this into a Pichia pastoris expression vector, and purified the recombinant peptide. Intraperitoneal injection of the recombinant GsMTx4 to rats significantly increased the mechanical threshold for paw withdrawal in Randall Sellito test, eliciting significant analgesic responses to inflammation-induced mechanical hyperalgesia. GsMTx4 also reduced mechanical allodynia induced by inflammation and by sciatic nerve injury in Von Frey test. However, the venom was ineffective at changing withdrawal latency in hot plate and tail-flick tests. These results suggest that GsMTx4 selectively alleviates mechanical hyperalgesia, which it presumably achieves by blocking mechanosensitive channels. Because the peptide venom induces analgesia for some forms of mechanical pain, GsMTx4 appears to have potential clinical use as a pain treatment. PMID:18359568

  7. Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys.

    Science.gov (United States)

    Warren, Travis K; Jordan, Robert; Lo, Michael K; Ray, Adrian S; Mackman, Richard L; Soloveva, Veronica; Siegel, Dustin; Perron, Michel; Bannister, Roy; Hui, Hon C; Larson, Nate; Strickley, Robert; Wells, Jay; Stuthman, Kelly S; Van Tongeren, Sean A; Garza, Nicole L; Donnelly, Ginger; Shurtleff, Amy C; Retterer, Cary J; Gharaibeh, Dima; Zamani, Rouzbeh; Kenny, Tara; Eaton, Brett P; Grimes, Elizabeth; Welch, Lisa S; Gomba, Laura; Wilhelmsen, Catherine L; Nichols, Donald K; Nuss, Jonathan E; Nagle, Elyse R; Kugelman, Jeffrey R; Palacios, Gustavo; Doerffler, Edward; Neville, Sean; Carra, Ernest; Clarke, Michael O; Zhang, Lijun; Lew, Willard; Ross, Bruce; Wang, Queenie; Chun, Kwon; Wolfe, Lydia; Babusis, Darius; Park, Yeojin; Stray, Kirsten M; Trancheva, Iva; Feng, Joy Y; Barauskas, Ona; Xu, Yili; Wong, Pamela; Braun, Molly R; Flint, Mike; McMullan, Laura K; Chen, Shan-Shan; Fearns, Rachel; Swaminathan, Swami; Mayers, Douglas L; Spiropoulou, Christina F; Lee, William A; Nichol, Stuart T; Cihlar, Tomas; Bavari, Sina

    2016-03-17

    The most recent Ebola virus outbreak in West Africa, which was unprecedented in the number of cases and fatalities, geographic distribution, and number of nations affected, highlights the need for safe, effective, and readily available antiviral agents for treatment and prevention of acute Ebola virus (EBOV) disease (EVD) or sequelae. No antiviral therapeutics have yet received regulatory approval or demonstrated clinical efficacy. Here we report the discovery of a novel small molecule GS-5734, a monophosphoramidate prodrug of an adenosine analogue, with antiviral activity against EBOV. GS-5734 exhibits antiviral activity against multiple variants of EBOV and other filoviruses in cell-based assays. The pharmacologically active nucleoside triphosphate (NTP) is efficiently formed in multiple human cell types incubated with GS-5734 in vitro, and the NTP acts as an alternative substrate and RNA-chain terminator in primer-extension assays using a surrogate respiratory syncytial virus RNA polymerase. Intravenous administration of GS-5734 to nonhuman primates resulted in persistent NTP levels in peripheral blood mononuclear cells (half-life, 14 h) and distribution to sanctuary sites for viral replication including testes, eyes, and brain. In a rhesus monkey model of EVD, once-daily intravenous administration of 10 mg kg(-1) GS-5734 for 12 days resulted in profound suppression of EBOV replication and protected 100% of EBOV-infected animals against lethal disease, ameliorating clinical disease signs and pathophysiological markers, even when treatments were initiated three days after virus exposure when systemic viral RNA was detected in two out of six treated animals. These results show the first substantive post-exposure protection by a small-molecule antiviral compound against EBOV in nonhuman primates. The broad-spectrum antiviral activity of GS-5734 in vitro against other pathogenic RNA viruses, including filoviruses, arenaviruses, and coronaviruses, suggests the

  8. Specific requirements of GS-R3 related to process implementation

    International Nuclear Information System (INIS)

    The PowerPoint presentation gives: an overview of IAEA management system requirements or topics; - the requirements specific to processes and process implementation; - the key practical challenge of using the process approach specified in IAEA SG GS-G3.1 and GS-G3.5. The following items are thoroughly discussed: - Requirements related to specific process implementation and developing processes; - Process management; Generic management; - System processes: - Control of documents; Control of products; Control of records; - Purchasing; - Communication; - Managing organizational change; - Other requirements concerning the process management system; - General management system; - Grading; - Documentation; - Fulfilling the requirements of interested parties; - Management responsibility; - Planning responsibility and authority for the management system monitoring and measurement; - Independent assessment; - Management system review; - Non-conformances, corrective and preventive actions; - Improvement key practical challenge of using the process approach specified in IAEA SG GS-G3.1 and GS-G3.5; - Key challenge: - Process common to all stages; - Phases of process development proposed by IAEA. The following conclusions complete the presentation: GS-R-3 sets basic requirements for process-based integrated management system; - Some key generic processes required, no specific process model favoured namely, no reference to management, core and support processes; - Up to organization to determine appropriate process model; - Easily applicable to a wide range of facilities and activities, including those of a regulatory body; - Specific requirements are found in specific Safety Guide. (author)

  9. TaGS5-3A, a grain size gene selected during wheat improvement for larger kernel and yield.

    Science.gov (United States)

    Ma, Lin; Li, Tian; Hao, Chenyang; Wang, Yuquan; Chen, Xinhong; Zhang, Xueyong

    2016-05-01

    Grain size is a dominant component of grain weight in cereals. Earlier studies have shown that OsGS5 plays a major role in regulating both grain size and weight in rice via promotion of cell division. In this study, we isolated TaGS5 homoeologues in wheat and mapped them on chromosomes 3A, 3B and 3D. Temporal and spatial expression analysis showed that TaGS5 homoeologues were preferentially expressed in young spikes and developing grains. Two alleles of TaGS5-3A, TaGS5-3A-T and TaGS5-3A-G were identified in wheat accessions, and a functional marker was developed to discriminate them. Association analysis revealed that TaGS5-3A-T was significantly correlated with larger grain size and higher thousand kernel weight. Biochemical assays showed that TaGS5-3A-T possesses a higher enzymatic activity than TaGS5-3A-G. Transgenic rice lines overexpressing TaGS5-3A-T also exhibited larger grain size and higher thousand kernel weight than TaGS5-3A-G lines, and the transcript levels of cell cycle-related genes in TaGS5-3A-T lines were higher than those in TaGS5-3A-G lines. Furthermore, systematic evolution analysis in diploid, tetraploid and hexaploid wheat showed that TaGS5-3A underwent strong artificial selection during wheat polyploidization events and the frequency changes of two alleles demonstrated that TaGS5-3A-T was favoured in global modern wheat cultivars. These results suggest that TaGS5-3A is a positive regulator of grain size and its favoured allele TaGS5-3A-T exhibits a larger potential application in wheat high-yield breeding. PMID:26480952

  10. 利润最大问题与弱DEA有效(C2GS2)%PROFIT MAXIMIZATION PROBLEM AND WEAK DEA EFFICIENCY(C2GS2)

    Institute of Scientific and Technical Information of China (English)

    吴文江

    2001-01-01

    对利润最大问题[1]本文用不同于文[1]的方法来讨论.通过解这一个问题,不但知道在一定条件下有最大利润的决策单元的弱DEA有效性(C2GS2),而且找到所有有最大利润的决策单元.

  11. Bioinformatics and protein modelling of the GS element of Mycobacteriumavium subsp. paratuberculosis (MAP) and GS-encoded proteins as drugtargets and vaccine components

    Institute of Scientific and Technical Information of China (English)

    Joe Sheridan; Tim Bull; Nazira Sumar; Jun Cheng; John Hermon-Taylor

    2000-01-01

    AIM To determine the function and cellular localization of GS-encoded proteins and to assess their potentialas drug targets and vaccine components.METHODS Bioinformatics software was used to predict the function of GS-encoded proteins and theirlocation within MAP. Protein modelling software was used to build protein structures.RESULTS The gene gsa is a truncated glycosyl transferase and probably non-functional. gsbA and gsbBproduce GDP-fucose which is methylated by gsc and acetylated by mpa. gsd is a fucosyl transferase whichattaches fucose to subterminal rhamnose on cell surface glycopeptidolipid. gsa, gsbA and gsbB and gsc arelocated within the cytoplasm. mpa is embedded in the plasma membrane with 10 transmembrane regions anda conspicuous extracellular loop. gsd is lipid-linked and predicted to localize to the microbial cell surface.CONCLUSION GS encodes the biosynthetic machinery to give MAP a surface coat of methylated andacetylated fucose which may contribute to its protease-resistant nature and ability to minimize immunerecognition. The gsbA/gsbB operon and gsd are promising drug targets and gsd is a good candidatecomponent of a new class of anti-MAP vaccines.

  12. 0gs+→21+ Transition Strengths in 106Sn and 108Sn

    International Nuclear Information System (INIS)

    The reduced transition probabilities, B(E2;0gs+→21+), have been measured in the radioactive isotopes 108,106Sn using subbarrier Coulomb excitation at the REX-ISOLDE facility at CERN. Deexcitation γ rays were detected by the highly segmented MINIBALL Ge-detector array. The results, B(E2;0gs+→21+)=0.222(19)e2b2 for 108Sn and B(E2;0gs+→21+)=0.195(39)e2b2 for 106Sn were determined relative to a stable 58Ni target. The resulting B(E2) values are ∼30% larger than shell-model predictions and deviate from the generalized seniority model. This experimental result may point towards a weakening of the N=Z=50 shell closure

  13. Correspondence of ISO 9001:2008 with IAEA GS-R-3

    International Nuclear Information System (INIS)

    This article identifies, characterises and analyses International Organisation for Standardisation (ISO) and International Atomic Energy Agency (IAEA) normative requirements for the development and implementation of quality management systems in nuclear organisations. The structures of the applicable standards, ISO 9001:2008 and IAEA GS-R-3, are characterised. The differences and similarities of objective, focus, scope, level of application, structure, vocabulary, management system, management responsibility, resource management, process implementation, measurement, assessment and improvement verified in the applicable standards are deeply reviewed. A correspondence of the applicable standards' requirements is presented topic by topic. A correlation of the applicable standards' requirements is made, showing IAEA GS-R-3 topics that have no correlation with ISO 9001:2008 and ISO 9001:2008 topics that have no correlation with IAEA GS-R-3. Conclusive remarks advise the pros and cons of using one of the applicable standards or the other. (author)

  14. Propiedades psicométricas del Maslach Burnout Inventory-GS en una muestra multiocupacional venezolana

    Directory of Open Access Journals (Sweden)

    Anthony Constant Millán de Lange

    2012-06-01

    Full Text Available Psychometric properties of the Maslach Burnout Inventory-GS in a Venezuelan sampleThe assessment of work stress is one of the most important aspects in the current scope of occupational psychological health, especially when it comes to chronic levels of involvement.Here are the results of the Venezuelan psychometric validation of the Cuban version of the Maslach Burnout Inventory-General Survey (MBI-GS. Results show adequate reliability of the instrument and suggest the need to remove item 1 to ensure its construct validity.Some indicators reveal the divergent criterion validity of the MBI-GS on the degree of the participant’s psychological well-being.

  15. Waveform Timing Performance of a 5 GS/s Fast Pulse Sampling Module with DRS4

    CERN Document Server

    Wang, Jinhong; An, Qi

    2015-01-01

    We first clarify timing issues of non-uniform sampling intervals regarding a 5 GS/s fast pulse sampling module with DRS4. Calibration strategy is proposed, and as a result, the waveform timing performance is improved to be below 10 ps RMS. We then further evaluate waveform timing performance of the module by comparing with a 10 GS/s oscilloscope in a setup with plastic scintillators and fast PMTs. Different waveform timing algorithms are employed for analysis, and the module shows comparable timing performance with that of the oscilloscope.

  16. Antifoaming materials studies in G.S. (Girlder sulfide) heavy water plants. Chemical stability. Pt. 1

    International Nuclear Information System (INIS)

    As in Girlder sulfide (G.S.) heavy water plants the system is inherently foaming, it is necessary to use antifoaming materials to solve this problem. This work describes the assays and results of some surfactants that can be employed to this purpose, under the corresponding temperature and pressure conditions of the plant. (Author)

  17. GS Soil - Assessment and strategic development of INSPIRE compliant Geodata-Services for European Soil Data

    DEFF Research Database (Denmark)

    Krogh, Paul Henning; Münier, Bernd

    GS Soil aims at establishing a Best Practice Network dealing with a cluster of the data themes listed in the Annexes I to III of the INSPIRE Directive (2007/2/EC) and focused on soil related issues. The establishment of the soil thematic strategy and the new transboundary EU approach for protected...

  18. GS143, an IκB ubiquitination inhibitor, inhibits allergic airway inflammation in mice

    International Nuclear Information System (INIS)

    Asthma is characterized by airway inflammation with intense eosinophil infiltration and mucus hyper-production, in which antigen-specific Th2 cells play critical roles. Nuclear factor-κB (NF-κB) pathway has been demonstrated to be essential for the production of Th2 cytokines and chemokines in the airways in murine asthma models. In the present study, we examined the effect of GS143, a novel small-molecule inhibitor of IκB ubiquitination, on antigen-induced airway inflammation and Th2 cytokine production in mice. Intranasal administration of GS143 prior to antigen challenge suppressed antigen-induced NF-κB activation in the lung of sensitized mice. Intranasal administration of GS143 also inhibited antigen-induced eosinophil and lymphocyte recruitment into the airways as well as the expression of Th2 cytokines and eotaxin in the airways. Moreover, GS143 inhibited antigen-induced differentiation of Th2 cells but not of Th1 cells in vitro. Taken together, these results suggest that IκB ubiquitination inhibitor may have therapeutic potential against asthma

  19. FLiGS Score: A New Method of Outcome Assessment for Lip Carcinoma–Treated Patients

    Directory of Open Access Journals (Sweden)

    Veronica Di Fede, MD

    2015-03-01

    Conclusions: FLiGS score is a simple way of assessing functional impairment related to lip cancer before and after surgery; it is sensitive, valid, reliable, and clinically relevant: it provides useful information to orient the physician in the postoperative management and in the rehabilitation program.

  20. Crystal structure of the β2 adrenergic receptor-Gs protein complex

    Energy Technology Data Exchange (ETDEWEB)

    Rasmussen, Søren G.F.; DeVree, Brian T; Zou, Yaozhong; Kruse, Andrew C; Chung, Ka Young; Kobilka, Tong Sun; Thian, Foon Sun; Chae, Pil Seok; Pardon, Els; Calinski, Diane; Mathiesen, Jesper M; Shah, Syed T.A.; Lyons, Joseph A; Caffrey, Martin; Gellman, Samuel H; Steyaert, Jan; Skiniotis, Georgios; Weis, William I; Sunahara, Roger K; Kobilka, Brian K [Brussels; (Trinity); (Michigan); (Stanford-MED); (Michigan-Med); (UW)

    2011-12-07

    G protein-coupled receptors (GPCRs) are responsible for the majority of cellular responses to hormones and neurotransmitters as well as the senses of sight, olfaction and taste. The paradigm of GPCR signalling is the activation of a heterotrimeric GTP binding protein (G protein) by an agonist-occupied receptor. The β2 adrenergic receptor (β2AR) activation of Gs, the stimulatory G protein for adenylyl cyclase, has long been a model system for GPCR signalling. Here we present the crystal structure of the active state ternary complex composed of agonist-occupied monomeric β2AR and nucleotide-free Gs heterotrimer. The principal interactions between the β2AR and Gs involve the amino- and carboxy-terminal α-helices of Gs, with conformational changes propagating to the nucleotide-binding pocket. The largest conformational changes in the β2AR include a 14Å outward movement at the cytoplasmic end of transmembrane segment 6 (TM6) and an α-helical extension of the cytoplasmic end of TM5. The most surprising observation is a major displacement of the α-helical domain of Gαs relative to the Ras-like GTPase domain. This crystal structure represents the first high-resolution view of transmembrane signalling by a GPCR.

  1. The 2015 hard-state only outburst of GS 1354-64

    Science.gov (United States)

    Stiele, H.; Kong, A. K. H.

    2016-07-01

    Since its outburst in 1997, GS 1354-64 stayed in quiescence. In 2015 June, renewed activity of GS 1354-64 was observed. Based on our analysis of energy spectra and timing properties obtained from Swift/X-ray telescope monitoring data, we found that GS 1354-64 stayed in the hard state during the entire outburst. Such a hard state only (or `failed' outburst) has also been observed in 1997. In addition, we analysed an XMM-Newton observation taken on August 6th. We compared variability on long and short time-scales using covariance ratio and found that the ratio showed a decrease towards lower energies instead of the increase that has been found in other black hole X-ray binaries. There are now two sources (H1743-322 and GS 1354-64) that do not show an increase towards lower energies in their covariance ratio. Both sources have been observed during `failed' outbursts and showed photon indices much harder than what is usually observed in black hole X-ray binaries.

  2. The 2015 hard-state only outburst of GS 1354-64

    CERN Document Server

    Stiele, H

    2016-01-01

    Since its outburst in 1997 GS 135-64 stayed in quiescence. In June 2015 renewed activity of GS 1354-64 was observed. Based on our analysis of energy spectra and timing properties obtained from Swift/XRT monitoring data we found that GS 1354-64 stayed in the hard state during the entire outburst. Such a hard state only (or "failed" outburst) has also been observed in 1997. In addition, we analysed an XMM-Newton observation taken on August 6th. We compared variability on long and short time scales using covariance ratio and found that the ratio showed a decrease towards lower energies instead of the increase that has been found in other black hole X-ray binaries. There are now two sources (H 1743-322 and GS 1354-64) that do not show an increase towards lower energies in their covariance ratio. Both sources have been observed during "failed" outbursts and showed photon indices much harder than what is usually observed in black hole X-ray binaries.

  3. Analysis of EMS Mutagenized Soybean by Combination of DOP-PCR and GS-FLX

    International Nuclear Information System (INIS)

    Ethylmethanesulfonate (EMS) has been commonly used to induce mutations for various organisms because it led to irreversible mutations with a high level of frequency. With relatively few individuals, saturated mutagenized populations could be generated by chemical mutagens. Since high-throughput sequencing instruments, such as GS-20 or GS-FLX from Roche/454 Life Sciences are now available, characterization of nucleic acids and massive mutant analysis are more feasible. Due to the requirement of sequence information and high cost for designing primers, degenerate oligonucleotide primed PCR (DOP-PCR) instead of direct sequencing was used for single nucleotide polymorphisms (SNPs) survey. In this study, we screen point mutation in soybean mutants generated by EMS mutagenesis using a combination of DOP-PCR methodology and GS-FLX. Four different modified DOP-PCR primers were used for amplifying genomic DNA of three soybean genotypes, Sinpaldalkong 2, SS2-2 and 25-1-1. Then, nucleotide sequences of these amplified PCR products were analyzed by GS-FLX. Different number and length of contigs and singlets were constructed depending on soybean genotypes and nucleotide identity, after sequences were trimmed and aligned. With 100% in identity, average 1,100 contigs and 7,000 singlets were formed in each soybean genotype. In order to survey sequence polymorphisms, POLYBAYES was used with base quality consideration. A total of 1,187 putative SNPs were detected, and these polymorphisms should be reconfirmed by direct sequencing after a homology search against GenBank databases. (author)

  4. Analysis of EMS mutagenized soybean by combination of DOP-PCR and GS-FLX

    International Nuclear Information System (INIS)

    Ethylmethanesulfonate (EMS) causing point mutations were commonly used to induce mutations for various organisms because this leaded to irreversible mutations with high level of frequency. With relatively few individuals, saturated mutagenized populations could be generated by chemical mutagens. Since high-throughput sequencing instruments, such as GS-20 or GS-FLX from Roche/454 Life Sciences, are now available, characterization of nucleic acids and massive mutant analysis are more feasible. Due to the requirement of sequence information and high cost for designing primers, degenerate oligonucleotide primed PCR (DOP-PCR) instead of direct sequencing was used for single nucleotide polymorphisms (SNPs) survey. In this study, we screen point mutation in soybean mutants generated by EMS mutagenesis using combination of DOP-PCR methodology and GS-FLX. Four different modified DOP-PCR primers were used for amplifying genomic DNA of three soybean genotypes, Sinpaldalkong 2, SS2-2 and 25-1-1. And then, nucleotide sequences of these amplified PCR products were analyzed by GS-FLX. Different number and length of contigs and singlets were constructed depending on soybean genotypes and nucleotide identity, after sequences were trimmed and aligned. With 100% in identity, average 1,100 contigs and 7,000 singlets were formed in each soybean genotype. In order to survey sequence polymorphisms, POLYBAYES was used with base quality consideration. A total of putative 1,187 SNPs were detected and these polymorphisms should be reconfirmed by direct sequencing after homology search against GenBank databases. (author)

  5. Formation of tabular single-domain magnetite induced by Geobacter metallireducens GS-15

    OpenAIRE

    Vali, Hojatollah; Weiss, Benjamin; LI, YI-LIANG; Sears, S. Kelly; Kim, Soon Sam; Kirschvink, Joseph L.; Zhang, Chuanlun L.

    2004-01-01

    Distinct morphological characteristics of magnetite formed intracellularly by magnetic bacteria (magnetosome) are invoked as compelling evidence for biological activity on Earth and possibly on Mars. Crystals of magnetite produced extracellularly by a variety of bacteria including Geobacter metallireducens GS-15, thermophilic bacteria, and psychrotolerant bacteria are, however, traditionally not thought to have nearly as distinct morphologies. The size and shape of extracellular magnetite dep...

  6. Highly efficient antibody immobilization with multimeric protein Gs coupled magnetic silica nanoparticles

    Science.gov (United States)

    Lee, J. H.; Choi, H. K.; Chang, J. H.

    2011-10-01

    This work reports the immobilization of monomeric, dimeric and trimer protein Gs onto silica magnetic nanoparticles for self-oriented antibody immobilization. To achieve this, we initially prepared the silica-coated magnetic nanoparticle having about 170 nm diameters. The surface of the silica coated magnetic nanoparticles was modified with 3- aminopropyl-trimethoxysilane (APTMS) to chemically link to multimeric protein Gs. The conjugation of amino groups on the SiO2-MNPs to cysteine tagged in multimeric protein Gs was performed using a sulfo-SMCC coupling procedure. The binding efficiencies of monomer, dimer and trimer were 77 %, 67 % and 55 % respectively. However, the efficiencies of antibody immobilization were 70 %, 83 % and 95 % for monomeric, dimeric and trimeric protein G, respectively. To prove the enhancement of accessibility by using multimeric protein G, FITC labeled goat-anti-mouse IgG was treated to mouse IgG immobilized magnetic silica nanoparticles through multimeric protein G. FITC labeled goat anti-mouse IgGs were more easily bound to mouse IgG immobilized by trimeric protein G than others. Finally protein G bound silica magnetic nanoparticles were utilized to develop highly sensitive immunoassay to detect hepatitis B antigen.

  7. Gsα deficiency in adipose tissue improves glucose metabolism and insulin sensitivity without an effect on body weight.

    Science.gov (United States)

    Li, Yong-Qi; Shrestha, Yogendra B; Chen, Min; Chanturiya, Tatyana; Gavrilova, Oksana; Weinstein, Lee S

    2016-01-12

    Gsα, the G protein that transduces receptor-stimulated cAMP generation, mediates sympathetic nervous system stimulation of brown adipose tissue (BAT) thermogenesis and browning of white adipose tissue (WAT), which are both potential targets for treating obesity, as well as lipolysis. We generated a mouse line with Gsα deficiency in mature BAT and WAT adipocytes (Ad-GsKO). Ad-GsKO mice had impaired BAT function, absent browning of WAT, and reduced lipolysis, and were therefore cold-intolerant. Despite the presence of these abnormalities, Ad-GsKO mice maintained normal energy balance on both standard and high-fat diets, associated with decreases in both lipolysis and lipid synthesis. In addition, Ad-GsKO mice maintained at thermoneutrality on a standard diet also had normal energy balance. Ad-GsKO mice had improved insulin sensitivity and glucose metabolism, possibly secondary to the effects of reduced lipolysis and lower circulating fatty acid binding protein 4 levels. Gsα signaling in adipose tissues may therefore affect whole-body glucose metabolism in the absence of an effect on body weight. PMID:26712027

  8. Digital post-calibration of a 5-bit 1.25 GS/s flash ADC

    International Nuclear Information System (INIS)

    We report a high-speed flash analog to digital converter (ADC) linearization technique employing the inverse Volterra model and digital post processing. First, a 1.25 GS/s 5-bit flash ADC is designed using a 0.18 μm CMOS, and the signal is quantized by a distributed track-and-hold circuit. Second, based on the Volterra series, a proposed digital post-calibration model is introduced. Then, the model is applied to estimate and compensate the nonlinearity of the high-speed flash ADC. Simulation results indicate that the distortion is reduced effectively. Specifically, the ADC achieves gains of 4.83 effective bits for a 117.1 MHz frequency input and 4.74 effective bits for a Nyquist input at 1.25 GS/s. (semiconductor integrated circuits)

  9. Digital post-calibration of a 5-bit 1.25 GS/s flash ADC

    Institute of Scientific and Technical Information of China (English)

    杨阳; 赵显利; 仲顺安; 李国峰

    2012-01-01

    We report a high-speed flash analog to digital converter (ADC) linearization technique employing the inverse Volterra model and digital post processing.First,a 1.25 GS/s 5-bit flash ADC is designed using a 0.18μm CMOS,and the signal is quantized by a distributed track-and-hold circuit.Second,based on the Volterra series,a proposed digital post-calibration model is introduced.Then,the model is applied to estimate and compensate the nonlinearity of the high-speed flash ADC.Simulation results indicate that the distortion is reduced effectively.Specifically,the ADC achieves gains of 4.83 effective bits for a 117.1 MHz frequency input and 4.74 effective bits for a Nyquist input at 1.25 GS/s.

  10. Waveform timing performance of a 5 GS/s fast pulse sampling module with DRS4

    Science.gov (United States)

    Wang, Jin-Hong; Liu, Shu-Bin; An, Qi

    2015-10-01

    We first clarify timing issues of non-uniform sampling intervals regarding a 5 GS/s fast pulse sampling module with DRS4. A calibration strategy is proposed, and as a result, the waveform timing performance is improved to below 10 ps RMS. We then further evaluate waveform-timing performance of the module by comparing with a 10 GS/s oscilloscope in a setup with plastic scintillators and fast PMTs. Different waveform timing algorithms are employed for analysis, and the module shows comparable timing performance with that of the oscilloscope. Supported by Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-N27), and National Natural Science Foundation of China (11175176)

  11. Coastal occupation at the GS-1 site, Cook's Bay, Mo'orea, Society Islands

    International Nuclear Information System (INIS)

    Excavations at the GS-1 site uncovered buried stratigraphic deposits of some depth. Site stratigraphy and artifact content suggests the area was first used as a coastal habitation prior to being covered by substantial colluvial deposits and in historic times, modern fill. Wood charcoal samples recovered from the site were identified to short lived species for AMS radiocarbon dating. The wood charcoal identifications were also used to identify indigenous versus introduced species in order to clarify the depositional events associated with the stratigraphic deposits. The lowest deposits at GS-1 date to as early as the 11th century AD. Colluvial deposits overlying the lowest strata were definitively related to human activities, as Polynesian introductions are present in the wood charcoal assemblages. Finally, the frequency and types of artifacts recovered suggest the area served as an adze manufacturing locale through time. (author). 35 refs., 5 figs., 5 tabs.

  12. Antifoaming materials in G.S. (Girlder sulfide) heavy water plants. Thermical stability. Pt. 2

    International Nuclear Information System (INIS)

    In Girlder sulfide (G.S.) heavy water plants hydrogen sulfide-water systems are inherentely foaming, so the adding of antifoaming materials is of great importance. These may be of high volatility, pyrolizable or chemically unstable in plant operation conditions (water and hydrogen sulfide at 2 MPa, up to 230 deg C). About twenty commercial surfactants were studied from the point of view of their thermical stability. (Author)

  13. Glutathione Preservation during Storage of Rat Lenses in Optisol-GS and Castor Oil

    DEFF Research Database (Denmark)

    Holm, Thomas; Brøgger-Jensen, Martin Rocho; Johnson, Leif;

    2013-01-01

    Glutathione concentration in the lens decreases in aging and cataractous lenses, providing a marker for tissue condition. Experimental procedures requiring unfrozen lenses from donor banks rely on transportation in storage medium, affecting lens homeostasis and alterations in glutathione levels....... The aim of the study was to examine the effects of Optisol-GS and castor oil on lens condition, determined from their ability to maintain glutathione concentrations....

  14. Metabolism and Pharmacokinetics of the Anti-Hepatitis C Virus Nucleotide Prodrug GS-6620

    OpenAIRE

    Murakami, Eisuke; Wang, Ting; Babusis, Darius; Lepist, Eve-Irene; Sauer, Dorothea; Park, Yeojin; Vela, Jennifer E.; Shih, Robert; Birkus, Gabriel; Stefanidis, Dimitrios; Kim, Choung U.; Cho, Aesop; Ray, Adrian S.

    2014-01-01

    The anti-hepatitis C virus nucleotide prodrug GS-6620 employs a double-prodrug approach, with l-alanine-isopropyl ester and phenol moieties attached to the 5′-phosphate that release the nucleoside monophosphate in hepatocytes and a 3′-isobutyryl ester added to improve permeability and oral bioavailability. Consistent with the stability found in intestinal homogenates, following oral administration, intact prodrug levels in blood plasma were the highest in dogs, followed by monkeys, and then w...

  15. FLiGS Score: A New Method of Outcome Assessment for Lip Carcinoma–Treated Patients

    OpenAIRE

    Veronica Di Fede, MD; Rita Grassi, MD; Francesca Toia, MD; Luigi Di Rosa, MD; Adriana Cordova, MD

    2015-01-01

    Background: Lip cancer and its treatment have considerable functional and cosmetic effects with resultant nutritional and physical detriments. As we continue to investigate new treatment regimens, we are simultaneously required to assess postoperative outcomes to design interventions that lessen the adverse impact of this disease process. We wish to introduce Functional Lip Glasgow Scale (FLiGS) score as a new method of outcome assessment to measure the effect of lip cancer and its treatment ...

  16. Model Innovation of Process Based on the Standard e-commerce International GS1

    OpenAIRE

    Oscar Sanjuan Martinez; Luz Andrea Rodríguez Rojas; Cristina Pelayo G. Bustelo; Giovanny Mauricio Tarazona Bermúdez

    2012-01-01

    This article focuses on the design and characterization of management model for MSMEs, based on e-commerce and the GS1 international e-com standard. The first part contextualizes electronic commerce and its impact on domestic industry, and briefly describes the B2B e-commerce model used in Colombia. Subsequently the first step to apply the model is presented, which corresponds to the design of a diagnostic methodology that evaluates the technological, technical, commercial and administrative ...

  17. Further evidence supporting a role for gs signal transduction in severe malaria pathogenesis.

    Directory of Open Access Journals (Sweden)

    Sarah Auburn

    Full Text Available With the functional demonstration of a role in erythrocyte invasion by Plasmodium falciparum parasites, implications in the aetiology of common conditions that prevail in individuals of African origin, and a wealth of pharmacological knowledge, the stimulatory G protein (Gs signal transduction pathway presents an exciting target for anti-malarial drug intervention. Having previously demonstrated a role for the G-alpha-s gene, GNAS, in severe malaria disease, we sought to identify other important components of the Gs pathway. Using meta-analysis across case-control and family trio (affected child and parental controls studies of severe malaria from The Gambia and Malawi, we sought evidence of association in six Gs pathway candidate genes: adenosine receptor 2A (ADORA2A and 2B (ADORA2B, beta-adrenergic receptor kinase 1 (ADRBK1, adenylyl cyclase 9 (ADCY9, G protein beta subunit 3 (GNB3, and regulator of G protein signalling 2 (RGS2. Our study amassed a total of 2278 cases and 2364 controls. Allele-based models of association were investigated in all genes, and genotype and haplotype-based models were investigated where significant allelic associations were identified. Although no significant associations were observed in the other genes, several were identified in ADORA2A. The most significant association was observed at the rs9624472 locus, where the G allele (approximately 20% frequency appeared to confer enhanced risk to severe malaria [OR = 1.22 (1.09-1.37; P = 0.001]. Further investigation of the ADORA2A gene region is required to validate the associations identified here, and to identify and functionally characterize the responsible causal variant(s. Our results provide further evidence supporting a role of the Gs signal transduction pathway in the regulation of severe malaria, and request further exploration of this pathway in future studies.

  18. The Research of Petroleum Enterprise Information System Architecture Based on the G/S Model

    Science.gov (United States)

    Rui, Liu; Xirong, Guo; Fang, Miao

    This paper explains the petroleum engineering technologies of petroleum enterprise supported by G/S model, which combine process of exploring, developing, and transporting of petroleum enterprise, these key technologies with spatial information technology supported by Digital Earth Platform, resulting in the improvement of the scientificity, accuracy, and rationality of the petroleum engineering technologies and the reduction of the cost and the increase of the benefits.

  19. Marketingová komunikace ve farmaceutickém průmyslu: GS CONDRO FORTE

    OpenAIRE

    Frýdlová, Jana

    2012-01-01

    The aim of this thesis is to present the theoretical attributes of marketing communications, as part of the marketing mix and in particular in relation to specific areas of the pharmaceutical industry. The practical part is focused on pharmaceutical product marketing communication towards the ultimate consumer. For this goal I chose Green pharmaceutical company - Swan Pharmaceuticals CR, as a nutritional supplement for her joints Condro Forte GS.

  20. Insulin activation of mouse diaphragm glycogen synthase (GS) involves generation of electrophoretically distinct subunit species

    International Nuclear Information System (INIS)

    Glycogen synthase, the rate limiting enzyme for glycogen synthesis, was analyzed in mouse diaphragm extracts both by immunoprecipitation and immunoblotting using specific antibodies raised to the rabbit muscle enzyme. Diaphragms, with the supporting ribs attached, were incubated either with or without [32P]P/sub i/ in the medium. In extracts from unincubated, rapidly frozen diaphragms, immunoblotting indicated the presence of 3 distinct species, separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). In addition, phosphorylation of immunoprecipitated GS with the kinase F/sub A//GSK-3 converted the higher mobility forms into the low mobility species. In diaphragms incubated with [32P]P/sub i/, 32P was incorporated only into one of the GS species, that of lowest mobility, indicating differential labelling among the multiple subunit forms. Insulin action, which increased the -/+ glucose-6-P activity ratio from 0.2 to 0.4, converted the low mobility species to the two higher mobility forms. The authors propose that this effect of insulin can be explained by dephosphorylation in the proline/serine rich site 3 region of GS, which has potent influence on both mobility on SDS-PAGE and activity

  1. Behind the scenes of GS: each to his own lock and key

    CERN Document Server

    Anaïs Schaeffer

    2014-01-01

    The GS-ASE team in charge of access control has an access solution for every type of premises, from radiation-controlled areas to simple administrative offices.   Although CERN is unquestionably open to the world, some of its doors must remain closed for a simple reason: as an industrial site, it must put safety and security at the top of its priorities and control access to certain areas in order to protect people and property from hazards. The AC (Access Control) section of the GS-ASE group uses a range of access control tools as the type of restrictions varies considerably according to the type of premises or area concerned: the requirements at the entrances to the different CERN sites may be quite different, for example, to those at the entrances to a specific building, a storage area, a workshop or an experiment hall within the fenced part of the CERN site. “We use different types of access systems at CERN”, explains Rui Nunes, head of the GS-ASE-AC section. &ldq...

  2. Genome sequencing and annotation of Laceyella sacchari strain GS 1-1, isolated from hot spring, Chumathang, Leh, India

    Directory of Open Access Journals (Sweden)

    Navjot Kaur

    2014-12-01

    Full Text Available We report the 3.3-Mb draft genome of Laceyella sacchari strain GS 1-1, isolated from hot spring water sample, Chumathang, Leh, India. Draft genome of strain GS 1-1 consists of 3, 324, 316 bp with a G + C content of 48.8% and 3429 predicted protein coding genes and 75 RNAs. Geobacillus thermodenitrificans strain NG80-2, Geobacillus kaustophilus strain HTA426 and Geobacillus sp. Strain G11MC16 are the closest neighbors of the strain GS 1-1.

  3. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15

    OpenAIRE

    Lovley, Derek R.; Lonergan, Debra J.

    1990-01-01

    The dissimilatory Fe(III) reducer, GS-15, is the first microorganism known to couple the oxidation of aromatic compounds to the reduction of Fe(III) and the first example of a pure culture of any kind known to anaerobically oxidize an aromatic hydrocarbon, toluene. In this study, the metabolism of toluene, phenol, and p-cresol by GS-15 was investigated in more detail. GS-15 grew in an anaerobic medium with toluene as the sole electron donor and Fe(III) oxide as the electron acceptor. Growth c...

  4. Tight genetic linkage of a glucosyltransferase and dextranase of Streptococcus mutans GS-5.

    Science.gov (United States)

    Burne, R A; Rubinfeld, B; Bowen, W H; Yasbin, R E

    1986-12-01

    A genetic library consisting of over 5000 clones with an average insert size of 6.9 kilobasepairs (kbp) of Streptococcus mutans GS-5 has been constructed in a bivalent plasmid vector pMK3, which is capable of replicating in Escherichia coli and Bacillus subtilis. The recombinant plasmid pSUCRI, containing a 6.0 kbp fragment of S. mutans GS-5 DNA, was the focus of this study. Using Southern hybridization, in vitro and in vivo gene expression techniques, and biochemical analysis, this clone was shown to encode the 55 kiloDalton (kDal) GS-5 gtfA gene product, as well as a 38 and a 66 kDal polypeptide. In addition to the gtfA gene, pSUCRI encodes a dextranase activity with specificity for alpha(1----6)-linked glucans, and with no detectable activity on mutan. The dextranase enzyme had an apparent molecular weight of 66 kDal as demonstrated by SDS-PAGE analysis of the proteins produced by a dextranase-negative deletion derivative. The pH optimum of the enzyme was approximately 6.0, and there was no detectable activity below pH 5.0. By subcloning various combinations of DNA fragments from pSUCRI, it was demonstrated that the dextranase gene (designated dexB) can be separated from the gtfA gene and still be efficiently expressed in both E. coli and B. subtilis. The dexB gene contained its own promoter and ribosome-binding site. The genetic linkage of the gtfA and dexB genes in the S. mutans GS-5 chromosome was confirmed by Southern hybridization and by the independent isolation of four distinct clones containing the gtfA gene and common flanking sequences. In addition to a glucosyltransferase and dextranase, an invertase-like activity is also encoded on pSUCRI, indicating that there is a cluster of genes on the S. mutans GS-5 chromosome which is devoted to the dissimilation of sucrose and concomitant synthesis or modification of glucans into a water-insoluble form, perhaps constituting an operon for glucan modification which can be coordinately regulated in response to

  5. On-Job-Training for Contract Specialists (GS-1102) at Fleet and Industrial Supply Center (FISC) Detachment Washington.

    OpenAIRE

    Stansfield, Patrick John.

    1995-01-01

    This thesis focused on the training requirements of GS-1102 Contract Specialists. In June 1995, a survey was taken of thirty-five contract specialists and ten supervisors at the Fleet and Industrial Supply Center, Norfolk, Washington Detachment. An analys

  6. Analysis of the Second Law of Thermodynamics applied to GS process in the primary production of heavy water

    International Nuclear Information System (INIS)

    An evaluation of the entropic change, through the analysis of the Second Law of Thermodynamic of GS process, with the aim of determine the sections where the majors irreversibilities of the process are located, is presented in this work. This process consist in the first enrichment stage of GS, which operates bithermally at 305 D and 403 Kelvin degree and a pressure of 2 MPa, participating four chemical compounds: H2O, HDO, H2S and HDS (Author)

  7. Gsα Deficiency in Adipose Tissue Leads to a Lean Phenotype with Divergent Effects on Cold Tolerance and Diet-Induced Thermogenesis

    OpenAIRE

    Chen, Min; Chen, Hui; Nguyen, Annie; Gupta, Divakar; Jie WANG; Lai, Edwin W.; Pacak, Karel; Gavrilova, Oksana; Michael J Quon; Weinstein, Lee S.

    2010-01-01

    Gsα, the G protein which mediates receptor-stimulated cAMP generation, has been implicated as a regulator of adipogenesis and adipose tissue function. Heterozygous Gsα mutations lead to obesity in Albright hereditary osteodystrophy (AHO) patients and in mice. In this study we generated mice with adipose-specific Gsα deficiency. Heterozygotes had 50% loss of Gsα expression in adipose tissue and no obvious phenotype, suggesting that adipose-specific Gsα deficiency is not the cause of obesity in...

  8. Glutathione preservation during storage of rat lenses in optisol-GS and castor oil.

    Directory of Open Access Journals (Sweden)

    Thomas Holm

    Full Text Available BACKGROUND: Glutathione concentration in the lens decreases in aging and cataractous lenses, providing a marker for tissue condition. Experimental procedures requiring unfrozen lenses from donor banks rely on transportation in storage medium, affecting lens homeostasis and alterations in glutathione levels. The aim of the study was to examine the effects of Optisol-GS and castor oil on lens condition, determined from their ability to maintain glutathione concentrations. METHODOLOGY/PRINCIPAL FINDINGS: Rat lenses were stored in the two types of storage media at varying time intervals up to 3 days. Glutathione concentration was afterwards determined in an enzymatic detection assay, specific for both reduced and oxidized forms. Lenses removed immediately after death exhibited a glutathione concentration of 4.70±0.29 mM. In vitro stored lenses in Optisol-GS lost glutathione quickly, ending with a concentration of 0.60±0.34 mM after 3 days while castor oil stored lenses exhibited a slower decline and ended at 3 times the concentration. A group of lenses were additionally stored under post mortem conditions within the host for 6 hours before its removal. Total glutathione after 6 hours was similar to that of lenses removed immediately after death, but with altered GSH and GSSG concentrations. Subsequent storage of these lenses in media showed changes similar to those in the first series of experiments, albeit to a lesser degree. CONCLUSIONS/SIGNIFICANCE: It was determined that storage in Optisol-GS resulted in a higher loss of glutathione than lenses stored in castor oil. Storage for more than 12 hours reduced glutathione to half its original concentration, and was considered unusable after 24 hours.

  9. Management System Standards: Comparison between IAEA GS-R-3 and ISO 9001:2008

    International Nuclear Information System (INIS)

    This Safety Report compares the requirements of IAEA Safety Standards Series No. GS-R-3, The Management System for Facilities and Activities, and ISO 9001:2008, Quality Management Systems - Requirements, and identifies the main differences between the two standards. It provides information and guidance on adding safety specific management system requirements to the ISO 9001:2008 standard, to ensure that safety can be achieved. The publication is intended primarily for use by owners, operators and employees of nuclear facilities and installations, and by regulatory bodies, suppliers, and research and development organizations.

  10. Score System Study for Hand-Extended Noodle Quality Based on HMW-GS Index in Wheat Flour

    Institute of Scientific and Technical Information of China (English)

    KANG Zhi-yu; WANG Jian-jun; SHANG Xun-wu

    2007-01-01

    Hand-extended noodle, a special kind of noodle, requires particular quality flour to make it. High molecular weight glutenin subunits (HMW-GS) in wheat are important protein subunits, which affect flour quality. To improve breeding and selection efficiency of wheat varieties which are used in making hand-extended noodle, 100 spring wheat varieties were selected to study the importance of HMW-GS on noodle quality score indexes such as color, appearance, taste agreeability, toughness, stickiness, smoothness, taste, and total score, through methods of quantity theory and statistic evaluation. It was shown that the hand-extended noodle quality score of HMW-GS 1, 2*, N, 7, 7 + 8, 17 + 18, 22, 2 + 10, 2 +11, 2+ 12, 5 + 10, and 10 was 5.40, 5.35, 0, 2.55, 2.56, 9.19, 0.05, 0.15, 1.49, 1.14, 10.00, and 5.14, respectively. The score system for hand-extended noodle quality based on HMW-GS index included eight multiple linear regression equations (R2 > 0.98).Hence, using the HMW-GS composition, the eight hand-extended noodle quality indexes would be forecasted exactly.Results indicated that ideal subunit compositions of HMW-GS for this special usage were composition 1, 17 + 18, 5 + 10, or composition 2*, 17 + 18, 5 + 10. This standard could be used on variety selection in the early generation of breeding crosses. HMW-GS 2 + 10, 2 + 11, and 2 + 12 were the least desirable subunits for hand-extended noodle, which should be avoided in wheat variety selection aimed for hand-extended noodle flour use.

  11. Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum

    Directory of Open Access Journals (Sweden)

    Chen Fanguo

    2007-05-01

    Full Text Available Abstract Background High molecular weight glutenin subunits (HMW-GS have been proved to be mostly correlated with the processing quality of common wheat (Triticum aestivum. But wheat cultivars have limited number of high quality HMW-GS. However, novel HMW-GS were found to be present in many wheat asymmetric somatic hybrid introgression lines of common wheat/Agropyron elongatum. Results To exploit how these new subunits were generated, we isolated HMW-GS genes from two sib hybrid lines (II-12 and 11-4-6 and compared them with those from their parents. The result shows that two genes of hybrid (H11-3-3 and H11-4-3 are directly introgressed from the donor parent Agropyron elongatum; one hybrid gene (H1Dx5 comes from point mutation of a parental wheat gene (1Dx2.1; two other hybrid genes (H1By8 and H1By16 are likely resulting from unequal crossover or slippage of a parental wheat gene (1By9.1; and the sixth novel hybrid gene (H1Dy12 may come from recombination between two parental genes. Conclusion Therefore, we demonstrate that novel HMW-GS genes can be rapidly created through asymmetric somatic hybridization in a manner similar with the evolution mechanism of these genes supposed before. We also described gene shuffling as a new mechanism of novel HMW-GS gene formation in hybrids. The results suggest that asymmetric somatic hybridization is an important approach for widening HMW-GS genebank of wheat quality improvement.

  12. Effect of HMW-GS 5+10 on Quality Parameters in Four Leading Wheat Cultivars

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yan-bin; SUN Lian-fa; XIN Wen-li; SONG Qin-jie; ZHANG Chun-li; ZHAO Hai-bin; XIAO Zhi-min; QI Shi-yu

    2003-01-01

    HMW-GS 5+ 10 was introduced to four major wheat cultivars Kehan9, Kefeng3, Longmai20and Kenda4 through 5 or 6 consecutive backcrosses. No significant difference in protein content and dry glutencontent was observed between cultivars containing 5+ 10 and 2+ 12 or 3+12 (P>0. 1). In cuitivars contai-ning HMW-GS 5 +10, the ratio of wet gluten content to dry gluten content was 2.9 -5.0 % (P<0.01) lowerthan the ratio from the cultivars containing 2+ 12 or 3+12, the ratio of Zeleny sedimentation volume to drygluten content was 4.5 - 13.4% (P<0. 05) higher. The degree of softening in cuitivars having 5+10 was 15- 25 FU (P<0.01) lower, while the maximum resistance was 82 - 193 EU (P<0.05) higher. Backcrosseswith biochemical marker assisted selection in the improvement of quality in wheat cultivars was discussed.

  13. The HI supershell GS 118+01-44 and its role in the interstellar medium

    CERN Document Server

    Suad, L A; Noriega-Crespo, A; Arnal, E M; Testori, J C; Flagey, N

    2016-01-01

    We carry out a multiwavelength study to characterize the HI supershell designated GS 118+01-44, and to analyse its possible origin. A multiwavlength study has been carried out to study the supershell and its environs. We performed an analysis of the HI, CO, radio continuum, and infrared emission distributions. The Canadian Galactic Plane Survey (CGPS) HI data reveals that GS 118+01-44 is centred at (l, b) = (117.7, 1.4) with a systemic velocity of -44.3 km/s. According to Galactic rotation models this structure is located at 3.0 +- 0.6 kpc from the Sun. There are several HII regions and three supernova remnants (SNRs) catalogued in the region. On the other hand, the analysis of the temperature spectral index distribution shows that in the region there is a predominance of non-thermal emission. Infrared emission shows that cool temperatures dominate the area of the supershell. Concerning the origin of the structure, we found that even though several OB stars belonging to Cas OB5 are located in the interior of ...

  14. The performance measure of GS-DG MOSFET: an impact of metal gate work function

    Science.gov (United States)

    Mohapatra, S. K.; Pradhan, K. P.; Sahu, P. K.; Kumar, M. R.

    2014-06-01

    The quantitative assessment of the nanoscale gate stack double gate (GS-DG) MOSFET performance values are numerically calculated with different gate metal work functions (Φ m = 4.52 eV, 4.6 eV, 4.7 eV). The effect of electrostatic control on dc, analog and RF figures of merit (FOMs) which includes subthreshold slope (SS), drain induced barrier lowering (DIBL), transconductance generation factor (TGF), early voltage (V EA), intrinsic gain (AV), cut off frequency (f T) and transconductance frequency product (TFP), gain frequency product (GFP) and gain transconductance frequency product (GTFP) have been investigated for the model GS-DG MOSFET. Higher TGF and AV was achieved with Φ m = 4.6 eV for the device. For a better comparison among the analog/RF FOMs, the threshold voltage (V th) is maintained at a constant value for different work function cases. To achieve a constant V th, the channel doping (NA) and source/drain doping (ND) is tuned accordingly for all device cases. Superior f T which is due to higher transconductance (g m) and lower output conductance (g d), was observed for the device. In addition, better gain performances i.e. GFP and GTFP were achieved resulting from improved g m. Thus, the device structure modelled with Φ m of 4.6 eV can be considered as a better candidate for analog and RF circuit applications.

  15. Behind the scenes of GS: a long-term urban planning vision

    CERN Multimedia

    Corinne Pralavorio

    2014-01-01

    The GS Department is working on a long-term plan for the urban planning of the CERN sites, ensuring that their development takes place using a coherent approach and keeping in mind the need for harmonious integration in the surrounding area.   Visitors are often bemused by CERN’s buildings, with their 1950s style and a layout that seems to defy all logic. Up until the 90s, buildings were erected as accelerators were built, with no apparent concern for harmony. But now, with a much larger number of users and installations, space is at a premium, getting around the site is difficult and urban planners are needed more than ever. "Over 9,000 people enter the CERN sites every day and we have 7,500 workstations," says Frédéric Magnin, Civil Engineering and Buildings Section Leader in the GS Department. For this reason, specialists in the department have been working on the development of the sites for five years. It is especially important that the...

  16. Glycation of polyclonal IgGs: Effect of sugar excipients during stability studies.

    Science.gov (United States)

    Leblanc, Y; Bihoreau, N; Jube, M; Andre, M-H; Tellier, Z; Chevreux, G

    2016-05-01

    A number of intravenous immunoglobulin preparations are stabilized with sugar additives that may lead over time to undesirable glycation reactions especially in liquid formulation. This study aimed to evaluate the reactivity of sugar excipients on such preparations in condition of temperature, formulation and concentration commonly used for pharmaceutical products. Through an innovative LC-MS method reported to characterize post-translational modifications of IgGs Fc/2 fragments, a stability study of IVIg formulated with reducing and non-reducing sugars has been undertaken. The rate of polyclonal IgGs glycation was investigated during 6months at 5, 25, 30 and 40°C. High levels of glycation were observed with reducing sugars such as glucose and maltose in the first months of the stability study from 25°C. Non-reducing sugars presented a low reactivity even at the highest tested temperature (40°C). Furthermore, a site by site analysis was performed by MS/MS to determine the glycation sites which were mainly identified at Lys246, Lys248 and Lys324. This work points out the high probability of glycation reactions in some commercialized products and describes a useful method to characterize IVIg glycated products issued from reducing sugar excipients. PMID:26992291

  17. Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?

    Directory of Open Access Journals (Sweden)

    Oscar Franzén

    2009-08-01

    Full Text Available Giardia intestinalis is a major cause of diarrheal disease worldwide and two major Giardia genotypes, assemblages A and B, infect humans. The genome of assemblage A parasite WB was recently sequenced, and the structurally compact 11.7 Mbp genome contains simplified basic cellular machineries and metabolism. We here performed 454 sequencing to 16x coverage of the assemblage B isolate GS, the only Giardia isolate successfully used to experimentally infect animals and humans. The two genomes show 77% nucleotide and 78% amino-acid identity in protein coding regions. Comparative analysis identified 28 unique GS and 3 unique WB protein coding genes, and the variable surface protein (VSP repertoires of the two isolates are completely different. The promoters of several enzymes involved in the synthesis of the cyst-wall lack binding sites for encystation-specific transcription factors in GS. Several synteny-breaks were detected and verified. The tetraploid GS genome shows higher levels of overall allelic sequence polymorphism (0.5 versus <0.01% in WB. The genomic differences between WB and GS may explain some of the observed biological and clinical differences between the two isolates, and it suggests that assemblage A and B Giardia can be two different species.

  18. Comparison between IAEA GS-R-3 and ISO 9001:2000

    International Nuclear Information System (INIS)

    International Atomic Energy Agency (IAEA) developed Safety Standard 'The Management System for Facilities and Activities' (GS-R-3) to define the requirements for establishing, implementing, assessing, and continually improving an integrated management system to integrate: safety, health, environmental, security, quality and economic elements. Standard requirements must be met to ensure the protection of people and the environment and they are governed by the objectives, concepts and IAEA Safety Fundamentals principles. The GS-R-3 requirements may be used as the basis for the: Management System of organizations directly responsible for operating facilities and activities and providing services; Regulation of these facilities and activities by the regulatory body: Management System of the relevant regulatory bodies; Operator, to specify to the supplier, via contractual documentation, any specific requirements that must be included in the supplier's management system for the supply and delivery of products. International Organization for Standardization (ISO) developed International Standard 'Quality Management Systems - Requirements' (ISO 9001:2000) to specify requirements for a quality management system where an organization: Needs to demonstrate its ability to consistently provide product that meets customer and applicable regulatory requirements, and Aims to enhance customer satisfaction through the effective application of the system, including processes for continual improvement of the system and the assurance of conformity to customer and applicable regulatory requirements. IAEA GS-R-3 is focused on meeting the overall safety requirements for the plant, personnel and society in general, and grading the application of the management system. Any State wishing to enter into an agreement with the IAEA concerning any form of Agency assistance is required to comply with the requirements of this safety standard that pertain to the activities covered by the agreement

  19. Study on forest landscape diversity based on ArcGIS and GS +

    Science.gov (United States)

    Zhang, Yujuan; Qu, Jianguang; Liu, Dandan; Yang, Jinling; Li, Dan

    2015-12-01

    This paper analyses the forest landscape diversity of the study area with the help of ArcGIS10 and GS+ software. The forest landscape diversity and spatial interpolation and spatial differentiation are also carried out. The result shows that the maximum value of SHDI in 1997is 2.0463 and the minimum value is 0.2544 , which are 1.9722 and 0.2418 in the year of 2009. The advantage religion of SHDI mainly distributes in the middle of the study region , showing a band region from southwest to northeast . The forest landscape diversity and the space location have a moderate spatial correlation and a obvious structural under a forest level.

  20. High Temperature Antenna Measurement System with GSG or GS Contact Probing Capability

    Science.gov (United States)

    Jordan, Jennifer L.; Scardelletti, Maximilian C.; Ponchak, George E.

    2009-01-01

    Applications that require data transmission at high temperatures are becoming more common due to growing commercial and military needs. Antennas are an indispensable part of these systems and the ability to characterize them at elevated temperatures is quite complicated with little or no information being reported on the subject [1]. This paper describes a measurement system that can characterize planar antennas up 600 C with ground-signal-ground (GSG) or ground-signal (GS) probe contacts. The return loss and radiation patterns of a folded slot antenna (FSA), designed to operate at 5 GHz (no ground plane on back side) and fabricated on an alumina substrate, are presented at room temperature (RT) and 250 C [2]. All measurements were made with Agilent's Precision Network Analyzer (PNA) E8361. The return loss and radiation patterns were also measured on a Styrofoam chuck to illustrate the effect the high temperature measurement system has on the patterns.

  1. Carbon steel protection in G.S. [Girldler sulphide] plants: Pt. 8

    International Nuclear Information System (INIS)

    In order to protect carbon steel of towers and piping of a GS experimental heavy water plant against corrosion produced by the action of aqueous solutions of hydrogen sulphide, a method, elsewhere published, was developed. Carbon steel exposed to saturated aqueous solutions of hydrogen sulphide forms iron sulphide scales. In oxygen free solutions, evolution of corrosion follows the sequence mackinawate → cubic ferrous sulphide → troilite → pyrrotite → pyrite. Scales formed by pyrrotite and pyrite are the most protective layers (these are obtained at 130 deg C, 2 MPa for a period of 14 days). Pyrite formation is favoured by an oxidizing agent presence that allows the oxidation of sulphur ions to disulphur ions. Elemental sulphur or oxygen were used as oxidating agents. Variation and operational parameters such as concentration, temperature, pH, aggregate time, etc. were studied. Though little improvement on protective scales quality was observed, results do not justify operational troubles and the additional costs and effort involved. (Author)

  2. An observation of GS 2000+25 with the LAC on board the 'GINGA' satellite

    International Nuclear Information System (INIS)

    The X-ray transient GS 2000+25 first appeared in Vulpecula on April 23, 1988. We report observations of this source with the Large Area Counter (LAC) on board the GINGA satellite. These observations were performed between April 30 and May 9, 1988 and on June 1, June 26 and September 8, 1988. The X-ray spectrum has an ultrasoft component and a hard tail. While the ultrasoft component has gradually decayed, the hard component has sometimes increased its intensity independently of the ultrasoft component. Furthermore. the hard component showed the large intensity variation on time scales of 1-100 sec, while the ultrasoft component was relatively stable on those time scales. (author)

  3. Purification and Characterization of an α-l-Arabinofuranosidase from Butyrivibrio fibrisolvens GS113

    OpenAIRE

    Hespell, Robert B.; O'Bryan, Patricia J.

    1992-01-01

    An α-l-arabinofuranosidase (EC 3.2.1.55) was purified from the cytoplasm of Butyrivibrio fibrisolvens GS113. The native enzyme had an apparent molecular mass of 240 kDa and was composed of eight polypeptide subunits of 31 kDa. The enzyme displayed an isoelectric point of 6.0, a pH optimum of 6.0 to 6.5, a pH stability of 4.0 to 8.0, and a temperature optimum of 45°C and was stable to 55°C. The Km and Vmax for p-nitrophenyl-α-l-arabinofuranoside were 0.7 mM and 109 μmol/min/mg of protein, resp...

  4. A 2 GS/s 8-bit folding and interpolating ADC in 90 nm CMOS

    International Nuclear Information System (INIS)

    A single-channel 2 GS/s 8-bit analog-to-digital converter in 90 nm CMOS process technology is presented. It utilizes cascade folding architecture, which incorporates an additional inter-stage sample-and-hold amplifier between the folding circuits to enhance the quantization time. It also uses the foreground on-chip digital-assisted calibration circuit to improve the linearity of the circuit. The post simulation results demonstrate that it has a differential nonlinearity < ±0.3 LSB and an integral nonlinearity < ±0.25 LSB at the Nyquist frequency. Moreover, 7.338 effective numbers of bits can be achieved at 2 GSPS. The whole chip area is 0.88 × 0.88 mm2 with the pad. It consumes 210 mW from a 1.2 V single supply. (semiconductor integrated circuits)

  5. Carbon steel protection in G.S. [Girldler sulphide] plants: Pt. 7

    International Nuclear Information System (INIS)

    In order to protect carbon steel towers and piping of a GS experimental heavy water plant against corrosion produced by the action of aqueous solutions of hydrogen sulphide, a method, elsewhere published, was developed. Carbon steel exposed to saturated aqueous solutions of hydrogen sulphide forms iron sulphide scales. In oxygen free solutions, evolution of corrosion follows the sequence mackinawite → cubic ferrous sulphide → troilite → pyrrotite → pyrite. Scales formed by pyrrotite and pyrite are the most protective layers (these are obtained at 130 deg C, 2 MPa for a period of 14 days). During a plant shutdown procedures, the carbon steel protected with those scales is exposed to water and highly humid air; under such conditions oxidation is unavoidable. Later, treatment in plant conditions does not regenerate scales because the composition of regenerated scales involves more soluble iron sulphides such as mackinawite and troilite. Therefore, it is not recommendable to expose the protective scales to atmospherical conditions. (Author)

  6. Development of a 1 GS/s high-resolution sampling ADC system

    International Nuclear Information System (INIS)

    Today, many experiments in high energy physics have to handle data rates of several MHz per readout channel to reach statistical significance for the measured quantities. In addition, these experiments frequently have to deal with short analog pulses of a few nanoseconds length which may cover large dynamic ranges and which require amplitude as well as time measurements with highest accuracy. For applications of this kind fast transient recorders with very high resolution and deep on-board memory are the first choice. We have built a 16-channel 12- or 14-bit single unit VME64x/VXS sampling ADC module which may sample at rates up to 1 GS/s. Fast algorithms have been developed and successfully tested on scintillating counter detectors to implement pulse shape discrimination, constant fraction timing and gated integration.

  7. Conformational changes in the G protein Gs induced by the β2 adrenergic receptor

    DEFF Research Database (Denmark)

    Chung, Ka Young; Rasmussen, Søren Gøgsig Faarup; Liu, Tong; Li, Sheng; DeVree, Brian T; Chae, Pil Seok; Calinski, Diane; Kobilka, Brian K; Woods, Virgil L; Sunahara, Roger K

    2011-01-01

    G protein-coupled receptors represent the largest family of membrane receptors that instigate signalling through nucleotide exchange on heterotrimeric G proteins. Nucleotide exchange, or more precisely, GDP dissociation from the G protein α-subunit, is the key step towards G protein activation and...... initiation of downstream signalling cascades. Despite a wealth of biochemical and biophysical studies on inactive and active conformations of several heterotrimeric G proteins, the molecular underpinnings of G protein activation remain elusive. To characterize this mechanism, we applied peptide amide......), we provide a rationale for a mechanism of nucleotide exchange, whereby the receptor perturbs the structure of the amino-terminal region of the α-subunit of Gs and consequently alters the 'P-loop' that binds the β-phosphate in GDP. As with the Ras family of small-molecular-weight G proteins, P...

  8. Anaerobic oxidation of toluene, phenol, and p-cresol by the dissimilatory iron-reducing organism, GS-15

    Science.gov (United States)

    Lovley, D.R.; Lonergan, D.J.

    1990-01-01

    The dissimilatory Fe(III) reducer, GS-15, is the first microorganism known to couple the oxidation of aromatic compounds to the reduction of Fe(III) and the first example of a pure culture of any kind known to anaerobically oxidize an aromatic hydrocarbon, toluene. In this study, the metabolism of toluene, phenol, and p-cresol by GS-15 was investigated in more detail. GS-15 grew in an anaerobic medium with toluene as the sole electron donor and Fe(III) oxide as the electron acceptor. Growth coincided with Fe(III) reduction. [ring-14C]toluene was oxidized to 14CO2, and the stoichiometry of 14CO2 production and Fe(III) reduction indicated that GS-15 completely oxidized toluene to carbon dioxide with Fe(III) as the electron acceptor. Magnetite was the primary iron end product during toluene oxidation. Phenol and p-cresol were also completely oxidized to carbon dioxide with Fe(III) as the sole electron acceptor, and GS-15 could obtain energy to support growth by oxidizing either of these compounds as the sole electron donor. p-Hydroxybenzoate was a transitory extracellular intermediate of phenol and p-cresol metabolism but not of toluene metabolism. GS-15 oxidized potential aromatic intermediates in the oxidation of toluene (benzylalcohol and benzaldehyde) and p-cresol (p-hydroxybenzylalcohol and p-hydroxybenzaldehyde). The metabolism described here provides a model for how aromatic hydrocarbons and phenols may be oxidized with the reduction of Fe(III) in contaminated aquifers and petroleum-containing sediments.

  9. Anaerobic oxidation of toluene, phenol, and p-cresol by the dissimilatory iron-reducing organism, GS-15

    International Nuclear Information System (INIS)

    The dissimilatory Fe(III) reducer, GS-15, is the first microorganism known to couple the oxidation of aromatic compounds to the reduction of Fe(III) and the first example of a pure culture of any kind known to anaerobically oxidize an aromatic hydrocarbon, toluene. In this study, the metabolism of toluene, phenol, and p-cresol by GS-15 was investigated in more detail. GS-15 grew in an anaerobic medium with toluene as the sole electron donor and Fe(III) oxide as the electron acceptor. Growth coincided with Fe(III) reduction. [ring-14C]toluene was oxidized to 14CO2, and the stoichiometry of 14CO2 production and Fe(III) reduction indicated that GS-15 completely oxidized toluene to carbon dioxide with Fe(III) as the electron acceptor. Magnetite was the primary iron end product during toluene oxidation. Phenol and p-cresol were also completely oxidized to carbon dioxide with Fe(III) as the sole electron acceptor, and GS-15 could obtain energy to support growth by oxidizing either of these compounds as the sole electron donor. p-Hydroxybenzoate was a transitory extracellular intermediate of phenol and p-cersol metabolism but not of toluene metabolism. GS-15 oxidized potential aromatic intermediates in the oxidation of toluene (benzylalcohol and benzaldehyde) and p-cersol (p-hydroxybenzylalchol and p-hydroxybenzaldehyde). The metabolism described here provides a model for how aromatic hydrocarbons and phenols may be oxidized with the reduction of Fe(III) in contaminated aquifers and petroleum-containing sediments

  10. Feather hydrolysate from Streptomyces sampsonii GS 1322: A potential low cost soil amendment.

    Science.gov (United States)

    Jain, Richa; Jain, Aakanchha; Rawat, Neha; Nair, Meera; Gumashta, Raghvendra

    2016-06-01

    Process parameters for obtaining hydrolysate from hen feathers, i.e., initial pH (5.0-9.0) and incubation period (1-6 day), were set and studied, using Streptomyces sampsonii GS 1322 in submerged and solid state conditions. Under submerged conditions, complete hydrolysis of feathers was observed on fifth day [initial pH 8.0, 28 ± 2°C, shaking (150 rpm)] with release of soluble protein (2985 μg ml(-1)) and amino acids (2407 μg ml(-1)). Cell free hydrolysate showed hydrolysis of casein (18 mm), gelatin (26 mm), collagen (31 mm), hemoglobin (23 mm) and Tween 80 (35 mm) while 445 U keratinase activity. Total soluble protein reached 5 mg ml(-1) in solid state conditions. During Pot experimentation using barren agriculture soil the effect of feather hydrolysate on wheat crop were recorded. Significant increase (pseed germination was observed in treated soils as compared to untreated. Treatment significantly increased plant height from 30 to 60 days and 30-90 days (p<0.001). Treated soil showed an increase in total microbial count, proteolytic activity, phosphate solubilizing bacteria and ammonifying bacteria, whereas pathogenic fungi load was reduced. S. sampsonii GS 1322 can be used for bio-processing of poultry wastes yielding feather hydrolysate rich in proteins and amino acids for development of low-cost organic amendment to accelerate wheat crop growth in barren agricultural land. PMID:26906933

  11. The Populus Superoxide Dismutase Gene Family and Its Responses to Drought Stress in Transgenic Poplar Overexpressing a Pine Cytosolic Glutamine Synthetase (GS1a)

    OpenAIRE

    Molina-Rueda, Juan Jesús; Tsai, Chung Jui; Kirby, Edward G.

    2013-01-01

    Background Glutamine synthetase (GS) plays a central role in plant nitrogen assimilation, a process intimately linked to soil water availability. We previously showed that hybrid poplar (Populus tremula X alba, INRA 717-1B4) expressing ectopically a pine cytosolic glutamine synthetase gene (GS1a) display enhanced tolerance to drought. Preliminary transcriptome profiling revealed that during drought, members of the superoxide dismutase (SOD) family were reciprocally regulated in GS poplar when...

  12. The better growth phenotype of DvGS1-transgenic arabidopsis thaliana is attributed to the improved efficiency of nitrogen assimilation

    Directory of Open Access Journals (Sweden)

    Zhu Chenguang

    2015-01-01

    Full Text Available The overexpression of the algal glutamine synthetase (GS gene DvGS1 in Arabidopsis thaliana resulted in higher plant biomass and better growth phenotype. The purpose of this study was to recognize the biological mechanism for the growth improvement of DvGS1-transgenic Arabidopsis. A series of molecular and biochemical investigations related to nitrogen and carbon metabolism in the DvGS1-transgenic line was conducted. Analysis of nitrogen use efficiency (NUE-related gene transcription and enzymatic activity revealed that the transcriptional level and enzymatic activity of the genes encoding GS, glutamate synthase, glutamate dehydrogenase, alanine aminotransferase and aspartate aminotransferase, were significantly upregulated, especially from leaf tissues of the DvGS1-transgenic line under two nitrate conditions. The DvGS1-transgenic line showed increased total nitrogen content and decreased carbon: nitrogen ratio compared to wild-type plants. Significant reduced concentrations of free nitrate, ammonium, sucrose, glucose and starch, together with higher concentrations of total amino acids, individual amino acids (glutamate, aspartate, asparagine, methionine, soluble proteins and fructose in leaf tissues confirmed that the DvGS1-transgenic line demonstrated a higher efficiency of nitrogen assimilation, which subsequently affected carbon metabolism. These improved metabolisms of nitrogen and carbon conferred the DvGS1-transgenic Arabidopsis higher NUE, more biomass and better growth phenotype compared with the wild-type plants.

  13. Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum

    OpenAIRE

    Chen Fanguo; Zhao Shuangyi; Liu Shuwei; Xia Guangmin

    2007-01-01

    Abstract Background High molecular weight glutenin subunits (HMW-GS) have been proved to be mostly correlated with the processing quality of common wheat (Triticum aestivum). But wheat cultivars have limited number of high quality HMW-GS. However, novel HMW-GS were found to be present in many wheat asymmetric somatic hybrid introgression lines of common wheat/Agropyron elongatum. Results To exploit how these new subunits were generated, we isolated HMW-GS genes from two sib hybrid lines (II-1...

  14. Growth hormone-releasing hormone resistance in pseudohypoparathyroidism type ia: new evidence for imprinting of the Gs alpha gene.

    Science.gov (United States)

    Mantovani, Giovanna; Maghnie, Mohamad; Weber, Giovanna; De Menis, Ernesto; Brunelli, Valeria; Cappa, Marco; Loli, Paola; Beck-Peccoz, Paolo; Spada, Anna

    2003-09-01

    Heterozygous inactivating mutations in the Gs alpha gene cause Albright's hereditary osteodystrophy. Consistent with the observation that only maternally inherited mutations lead to resistance to hormone action [pseudohypoparathyroidism type Ia (PHP Ia)], recent studies provided evidence for a predominant maternal origin of Gs alpha transcripts in endocrine organs, such as thyroid, gonad, and pituitary. The aim of this study was to investigate the presence of pituitary resistance to hypothalamic hormones acting via Gs alpha-coupled receptors in patients with PHP Ia. Six of nine patients showed an impaired GH responsiveness to GHRH plus arginine, consistent with a complete GH deficiency (GH peak from 2.6-8.6 microg/liter, normal > 16.5), and partial (GH peak 13.9 and 13.6 microg/liter) and normal responses were found in two and one patient, respectively. Accordingly, IGF-I levels were below and in the low-normal range in seven and two patients. All patients had a normal cortisol response to 1 microg ACTH test, suggesting a normal corticotroph function that was confirmed by a normal ACTH and cortisol response to CRH test in three patients. In conclusion, we report that in addition to PTH and TSH resistance, patients with PHP Ia display variable degrees of GHRH resistance, consistent with Gs alpha imprinting in human pituitary. PMID:12970263

  15. Amelioration of radiation esophagitis by orally administered p53/Mdm2/Mdm4 inhibitor (BEB55) or GS-nitroxide

    NARCIS (Netherlands)

    Kim, Hyun; Bernard, Mark E.; Epperly, Michael W.; Shen, Hongmei; Amoscato, Andrew; Dixon, Tracy M.; Doemling, Alexander S.; Li, Song; Gao, Xiang; Wipf, Peter; Wang, Hong; Zhang, Xichen; Kagan, Valerian E.; Greenberger, Joel S.

    2011-01-01

    Background/Aim: Esophagitis is a significant toxicity of radiation therapy for lung cancer. In this study, reduction of irradiation esophagitis in mice, by orally administered p53/Mdm2/Mdm4 inhibitor, BEB55, or the GS-nitroxide, JP4-039, was evaluated. Materials and Methods: BEB55 or JP4-039 in F15

  16. Apples with Oranges: Comparing the GS-FLX vs Ion Torrent Platforms for 16S Metagenomics Studies

    OpenAIRE

    Paech, Sam; McDermott, Stewart; Men, Artem; McGrath, Ken

    2012-01-01

    Decreasing costs and faster turn-around times mean that next-generation platforms are more readily accessible to metagenomics researchers. We compared the suitability of the GS-FLX (XLR70) and Ion Torrent (314 with 100bp chemistry) to generate data sets that are suitable for 16S-based taxonomic metagenomics.

  17. Effect of HMW-GS 6 + 8 and 1.5 +10 from Synthetic Hexaploid Wheat on Wheat Quality Traits

    Institute of Scientific and Technical Information of China (English)

    TANG Yong-lu; YANG Wu-yun; TIAN Ji-chun; LI Jun; CHEN Fang

    2008-01-01

    To determine the effect of 6 + 8 and 1.5 +10 HMW-GS of synthetic hexaploid wheat (SHW) on main quality parameters of wheat, a set of recombinant inbred lines (RILs) derived from the cross between a SHW with N, 6 + 8, 1.5+10 HMW-GS and a cultivar Chuanyu 12-1 (CY12-1) with 1, 7 + 8, 2 +12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption (FWA), and farinograph softening (SOF) but beyond parents at dough stability time (DST), breakdown time (BRT), quality number (QN), noodle score (NS), and loaf volume (LOV). All of the quality traits, especially in grain hardness (GH), zeleny sedimentation volume (SED), and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus (Glu-Al or Glu-Bl or Glu-Dl) on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 + 8 was better than 7 + 8 and there was no difference between 1.5 + 10 and 2+12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 + 8, 1.5 + 10 had the highest LOV and bread score (BS) values, whereas the components of 1, 7 + 8 and 1.5 + 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number (FN) and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6+8 from SHW had better overall quality characteristics than 7 + 8, whereas the effects of 1.5 +10 on quality was different in

  18. X-Ray Transient in Musca (GRS 1121-68 = GS 1124-683)

    DEFF Research Database (Denmark)

    Lund, Niels; Brandt, Søren; Makino, F.;

    1991-01-01

    Crab and increasing slowly during the observation. The source remained at a constant intensity level on Jan. 10. On Jan. 5 this source was not bright enough to be detectable by WATCH. The preliminary position is R.A. = 11h21m, Decl. = -68.1 deg (equinox 1950.0; uncertainty about 1 deg). We have not...... found any catalogued hard x-ray sources within our error circle; however, close to the given position, we have noted the existence of a radio pulsar (at R.A. = 11h10m, Decl. = -69 deg) with a period of 0.8 s, and a gamma-ray burst source, GRB 820829B, detected by Venera 13/14 in 1982. We have not...... detected any strong pulsations from the new source, but the time resolution of the available data does not allow a search for pulsations with periods shorter than about 20 s." F. Makino and the Ginga Team, Institute of Space and Astronautical Science, telex: "A bright transient x-ray source, designated GS...

  19. The Spin of the Black Hole GS 1124-683: Observation of a Retrograde Accretion Disk?

    CERN Document Server

    Morningstar, Warren R; Reis, Rubens C; Ebisawa, Ken

    2014-01-01

    We re-examine archival Ginga data for the black hole binary system GS 1124-683, obtained when the system was undergoing its 1991 outburst. Our analysis estimates the dimensionless spin parameter a=cJ/GM^2 by fitting the X-ray continuum spectra obtained while the system was in the "Thermal Dominant" state. For likely values of mass and distance, we find the spin to be a=-0.25 (-0.64, +0.05) (90% confidence), implying that the disk is retrograde (i.e. rotating antiparallel to the spin axis of the black hole). We note that this measurement would be better constrained if the distance to the binary and the mass of the black hole were more accurately determined. This result is unaffected by the model used to fit the hard component of the spectrum. In order to be able to recover a prograde spin, the mass of the black hole would need to be at least 15.25 Msun, or the distance would need to be less than 4.5 kpc, both of which disagree with previous determinations of the black hole mass and distance. If we allow f_col ...

  20. Behind the scenes of GS: a professional fire and rescue service like no other

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    Meet the professionals at the top of their game working hard for the well-being of the CERN community. Meet the CERN Fire Brigade.   Highly-qualified professional firefighters CERN’s Fire and Rescue Service (FRS, GS-FB Group) currently consists of 58 professionals. These are highly sought-after positions: for just a handful of vacancies, hundreds of applications are submitted from all over Europe. And bear in mind that only professional firefighters with at least five years’ experience at a centre with high levels of operational activity may apply… and they must also have a good command of at least one of the Organization's two official languages. Once the pre-selection process is over, around twenty candidates progress to the next stage, where they are really put through their paces with language tests, theory exams and exercises on real fires. “For the practical part, all candidates are evaluated on their responses to a real fire," ...

  1. Behind the scenes of GS: there’s only one way to go

    CERN Multimedia

    Anaïs Schaeffer

    2014-01-01

    At CERN, all of the Laboratory’s imports and exports are routed in the same way: through the Logistics Service. This GS-IS Group service is responsible for receiving, inspecting and distributing all goods sent to the Organization.   Whether products for the CERN Stores, components for the experiments, tools, machinery and materials for the workshops or equipment for users and members of the personnel, nothing escapes the attention of CERN’s Logistics Service, which every year processes nearly 70,000 incoming deliveries, 7,500 shipments and 160,000 distributed items. “The vast majority of our imports come from CERN Member States,” says imports and customs procedures manager Laurence Planque, “but we are receiving more and more goods for collaborators working at CERN from non-Member States such as China, India and Pakistan. All these imports are entitled to diplomatic exemption, so every day we have to manage the customs clearance procedures wit...

  2. THE SPIN OF THE BLACK HOLE GS 1124–683: OBSERVATION OF A RETROGRADE ACCRETION DISK?

    International Nuclear Information System (INIS)

    We re-examine archival Ginga data for the black hole binary system GS 1124–683, obtained when the system was undergoing its 1991 outburst. Our analysis estimates the dimensionless spin parameter a * = cJ/GM2 by fitting the X-ray continuum spectra obtained while the system was in the ''thermal dominant'' state. For likely values of mass and distance, we find the spin to be a∗=−0.25−0.64+0.05 (90% confidence), implying that the disk is retrograde (i.e., rotating antiparallel to the spin axis of the black hole). We note that this measurement would be better constrained if the distance to the binary and the mass of the black hole were more accurately determined. This result is unaffected by the model used to fit the hard component of the spectrum. In order to be able to recover a prograde spin, the mass of the black hole would need to be at least 15.25 M ☉, or the distance would need to be less than 4.5 kpc, both of which disagree with previous determinations of the black hole mass and distance. If we allow f col to be free, we obtain no useful spin constraint. We discuss our results in the context of recent spin measurements and implications for jet production

  3. Behind the scenes of GS: Did you say “grey”?

    CERN Multimedia

    Antonella Del Rosso

    2014-01-01

    Walking around the CERN site, what we tend to notice are the buildings, roads, and car parks. At first glance, any “green” seems to be in the minority. The reality is quite different, as developed land takes up only 40 of the almost 650 hectares made available to the Organization by its Host States.   1,300 trees in CERN's enclosed areas, 250 hectares of cultivated fields and meadows, 140 hectares of woodland, and three wetlands: CERN clearly deserves its “green” certification from the Swiss conservation foundation Nature et économie that it has held since 2009. "We maintain every site, but each site is different and has its own conservation criteria," says Mathieu Fontaine, head of Green Spaces in the Civil Engineering and Buildings section of the GS Department. At CERN, an external company takes care of the day-to-day upkeep, but more complex operations are often necessary. The various projects to fell diseased or ...

  4. S08: investigation and repair of a cracked feeder at Point Lepreau GS

    International Nuclear Information System (INIS)

    Early in 1997 investigation of a low level leak in the Point Lepreau GS (PLGS) PHTS revealed that an outlet feeder, S08, was leaking. Ultrasonic inspection, and subsequent failure analysis, revealed that the leak was a consequence of a crack. Given the unusual nature of this event, and current concerns over feeder thinning, a detailed and careful removal and examination procedure was developed. The S08 outlet feeder was removed and shipped to Chalk River Laboratories for examination. The examination confirmed that the failure was a through-wall crack, most likely the consequence of stress corrosion cracking. A critical point of the analysis was to determine how the crack initiated, and subsequently propagated. High residual stresses and possible abnormal loading in conjunction with chemistry environments resulted in the Stress Corrosion Cracking (SCC) of the S08 outlet feeder bend. It is recognized that some of the causative factors implicated in the S08 failure apply to other outlet feeders. In particular, residual stresses in the non-stress-relieved, short-radius cold bent pipes will remain relatively high over the future life of the feeders. However, the risk of CANDU feeder failure by SCC is judged to be extremely low based on the evidence of the inspections carried out to date and the good performance record of feeder pipe in the CANDU industry. The channel was restored to its locked configuration, and the failed section of feeder replaced. (author)

  5. Development of a 1 GS/s high-resolution transient recorder

    CERN Document Server

    Bartknecht, S; Herrmann, F; Königsmann, K; Lauser, L; Schill, C; Schopferer, S; Wollny, H

    2009-01-01

    With present-day detectors in high energy physics one is often faced with short analog pulses of a few nanoseconds length which may cover large dynamic ranges. In many experiments both amplitude and timing information have to be measured with high accuracy. Additionally, the data rate per readout channel can reach several MHz, which makes high demands on the separation of pile-up pulses. For such applications we have built the GANDALF transient recorder with a resolution of 12bit@1GS/s and an analog bandwidth of 500 MHz. Signals are digitized and processed by fast algorithms to extract pulse arrival times and amplitudes in real-time and to generate experiment trigger signals. With up to 16 analog channels, deep memories and a high data rate interface, this 6U-VME64x/VXS module is not only a dead-time free digitization unit but also has huge numerical capabilities provided by the implementation of a Virtex5-SXT FPGA. Fast algorithms implemented in the FPGA may be used to disentangle possible pile-up pulses and...

  6. Identification of the in vitro phosphorylation sites on Gs alpha mediated by pp60c-src.

    Science.gov (United States)

    Moyers, J S; Linder, M E; Shannon, J D; Parsons, S J

    1995-01-15

    Overexpression of pp60c-src in mouse fibroblasts potentiates both agonist-induced signalling through beta-adrenergic receptors and cyclic AMP accumulation in response to cholera toxin [Bushman, Wilson, Luttrell, Moyers and Parsons (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 7462-7466; Moyers, Bouton and Parsons (1993) Mol. Cell. Biol. 13, 2391-2400]. In reconstitution experiments in vitro, phosphorylation of Gs alpha by immune-complexed pp60c-src resulted in enhanced rates of receptor-mediated guanosine 5'-[gamma-thio]triphosphate (GTP[S]) binding and GTP hydrolysis [Hausdorff, Pitcher, Luttrell, Linder, Kurose, Parsons, Caron and Lefkowitz (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5720-5724]. These results suggest that one mechanism by which pp60c-src affects signalling through the beta-adrenergic receptor is by phosphorylation and functional alteration of the G protein. To elucidate how phosphorylation of Gs alpha might affect its function, we subjected phosphorylated, recombinant Gs alpha to tryptic phosphopeptide analysis. Phosphotryptic peptides were purified by h.p.l.c. and analysed by Edman degradation to determine the cycle numbers at which radiolabelled phosphotyrosine was released. Candidate peptides that contained Tyr residues at the corresponding positions were synthesized, phosphorylated in vitro by pp60c-src, and their migrations in two-dimensional electrophoresis/t.l.c. were compared with those of tryptic phosphopeptides from intact Gs alpha. We report here that Gs alpha is phosphorylated on two residues by pp60c-src, namely, Tyr-37 and Tyr-377. Tyr-37 lies near the site of beta gamma binding in the N-terminus, within a region postulated to modulate GDP dissociation and activation by GTP [Johnson, Dhanasekaran, Gupta, Lowndes, Vaillancourt and Ruoho (1991) J. Cell Biochem. 47, 136-146], while Tyr-377 is located in the extreme C-terminus, within a region of Gs alpha important for receptor interaction [Sullivan, Miller, Masters, Beiderman, Heideman and

  7. Structure determination and biochemical characterization of a putative HNH endonuclease from Geobacter metallireducens GS-15.

    Directory of Open Access Journals (Sweden)

    Shuang-yong Xu

    Full Text Available The crystal structure of a putative HNH endonuclease, Gmet_0936 protein from Geobacter metallireducens GS-15, has been determined at 2.6 Å resolution using single-wavelength anomalous dispersion method. The structure contains a two-stranded anti-parallel β-sheet that are surrounded by two helices on each face, and reveals a Zn ion bound in each monomer, coordinated by residues Cys38, Cys41, Cys73, and Cys76, which likely plays an important structural role in stabilizing the overall conformation. Structural homologs of Gmet_0936 include Hpy99I endonuclease, phage T4 endonuclease VII, and other HNH endonucleases, with these enzymes sharing 15-20% amino acid sequence identity. An overlay of Gmet_0936 and Hpy99I structures shows that most of the secondary structure elements, catalytic residues as well as the zinc binding site (zinc ribbon are conserved. However, Gmet_0936 lacks the N-terminal domain of Hpy99I, which mediates DNA binding as well as dimerization. Purified Gmet_0936 forms dimers in solution and a dimer of the protein is observed in the crystal, but with a different mode of dimerization as compared to Hpy99I. Gmet_0936 and its N77H variant show a weak DNA binding activity in a DNA mobility shift assay and a weak Mn²⁺-dependent nicking activity on supercoiled plasmids in low pH buffers. The preferred substrate appears to be acid and heat-treated DNA with AP sites, suggesting Gmet_0936 may be a DNA repair enzyme.

  8. Mutations of the Gs α-subunit gene in Albright hereditary osteodystrophy detected by denaturing gradient gel electrophoresis

    International Nuclear Information System (INIS)

    Affected members of most kindreds with Albright hereditary osteodystrophy have a partial deficiency of functional Gs, the guanine nucleotide-binding protein that stimulates adenylyl cyclase. By use of the polymerase chain reaction to amplify genomic fragments with the attachment of a high-melting G+C-rich region (GC clamp) and analysis of these fragments by denaturing gradient gel electrophoresis, heterozygous mutations in the Gs α-subunit at the donor splice junction of intron 10 and a coding frameshift created by a single base deletion within exon 10. The findings illustrate the heterogeneity of genetic defects in Albright hereditary osteodystrophy and the usefulness of the polymerase chain reaction-denaturing gradient gel electrophoresis method to search rapidly for mutations in a large candidate gene

  9. A high-yielding, generic fed-batch process for recombinant antibody production of GS-engineered cell lines

    DEFF Research Database (Denmark)

    Fan, Li; Zhao, Liang; Sun, Yating;

    2009-01-01

    An animal component-free and chemically defined fed-batch process for GS-engineered cell lines producing recombinant antibodies has been developed. The fed-batch process relied on supplying sufficient nutrients to match their consumption, simultaneously minimizing the accumulation of byproducts....... Compared to batch cultures, the fed-batch technology generated the magnitude of the increase in cell yields (5 fold) and final antibody concentrations (4-8 fold). The majority of the increase in final antibody concentration was functions of the increased cell density and the prolonged culture time. This...... generic and high-yielding fed-batch process would shorten development time, and ensure process stability, thereby facilitating the manufacture of therapeutic antibodies by GS-engineered cell lines....

  10. [Effects of pingyangmycin on the growth and differentiation of the transplantable squamous cell carcinoma of forestomach (GS-742) in mice].

    Science.gov (United States)

    Wang, F R

    1989-09-01

    The effect of pinyangmycin (PYM) on the growth and differentiation of the transplantable squamous cell carcinoma of forestomach (GS-742) in mice was studied by optical and electron microscope. The results showed that PYM had marked growth inhibitory effect on GS-742. Histopathologically, tumor necrosis, fibrosis and infiltration of lymphocytes were obvious in mice treated with PYM. A decrease in mitosis and increase in keratinization were also conspicuous. Ultrastructurally, microvilli on tumor cells were significantly reduced in number with formation of blebs and ruffles; mitochondria were swollen; endoplasmic reticulum was often decreased and dilated; and desmosome and tonofibrils were increased. The results indicate that PYM exerts its growth-inhibitory effect on tumor cells by cell damage, inhibition of mitosis and promotion of differentiation. PMID:2482804

  11. Antifoaming materials studies in G.S. (Girlder sulfide) heavy water plants. Chemical and thermical stability. Pt. 3

    International Nuclear Information System (INIS)

    In Girlder sulfide (G.S.) heavy water plants hydrogen sulfide-water systems are inherentely foaming, so the adding of antifoaming materials is of great importance. These may be of high volatility, pyrolizable or chemically unstable in plant operation conditions (water and hydrogen sulfide at 2 MPa, up to 230 deg C). Five commercial surfactants were studied from the point of view of their chemical and thermical stability in order to select the most suitable. (Author)

  12. Quality differences between NILs of wheat variety Long 97-586 possessing HMW-GS 7+8 and 7

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The high molecular weight glutenin subunits (HMW-GS) 7+8 were introduced into the Long 97–586 (1,7,2+12) wheat variety (Triticum aestivum) by 5 consecutive backcrosses with biochemical marker–assisted selection.Nearly isogenic lines (NILs) of HMW-GS 7 and 7+8 were obtained,and the NILs were planted in the experimental field at the Crop Breeding Institute of Heilongjiang Academy of Agricultural Science in 2004–2006.The field experiments were designed using the two-column contrast arrangement method with six replicates in 2004–2005 and four replicates in 2006.The result of three years experiments showed that the differences between NILs of Long 97–586 with subunit 7 and those with subunits 7+8 in the quality parameters of flour protein content and dry gluten content were negligible (P>0.1).However,the differences in some of the quality parameters were remarkably significant (P<0.01),including wet gluten content,ratio of wet gluten/dry gluten,gluten index,Zeleny sedimentation,ratio of sedimentation/dry gluten,and the farinogram parameters of water absorption,development time,stability,breakdown time and degree of softening.The difference between NILs with subunits 7+8 and subunit 7 was significant (P<0.05) on the alveogram W value and had a critical value (P=0.05) on the alveogram P value in 2006.The results show that HMW-GS 7+8 is far superior to HMW-GS 7 in terms of baking quality.The possibilities of using subunits 7+8 and subunit 7 in breeding strong and weak gluten wheat varieties are discussed in this paper.

  13. Studies on silicone based antifoaming agents to be used in G.S. (Girlder sulfide) heavy water plants

    International Nuclear Information System (INIS)

    In Girlder sulfide (G.S.) heavy water plants hydrogen sulfide-water systems are inherentely foaming, so the adding of antifoaming materials is of great importance. These may be of high volatility, pyrolizable or chemically unstable in plant operation conditions (water and hydrogen sulfide at 2MPa, up to 230 deg C). Six commercial silicone based antifoaming agents were studied from the point of view of their chemical and thermical stability in order to select the most suitable. (Author)

  14. Engineering the melanocortin-4 receptor to control constitutive and ligand-mediated G(S signaling in vivo.

    Directory of Open Access Journals (Sweden)

    Supriya Srinivasan

    Full Text Available The molecular and functional diversity of G protein-coupled receptors is essential to many physiological processes. However, this diversity presents a significant challenge to understanding the G protein-mediated signaling events that underlie a specific physiological response. To increase our understanding of these processes, we sought to gain control of the timing and specificity of G(s signaling in vivo. We used naturally occurring human mutations to develop two G(s-coupled engineered receptors that respond solely to a synthetic ligand (RASSLs. Our G(s-coupled RASSLs are based on the melanocortin-4 receptor, a centrally expressed receptor that plays an important role in the regulation of body weight. These RASSLs are not activated by the endogenous hormone alpha-melanocyte-stimulating hormone but respond potently to a selective synthetic ligand, tetrahydroisoquinoline. The RASSL variants reported here differ in their intrinsic basal activities, allowing the separation of the effects of basal signaling from ligand-mediated activation of the G(s pathway in vivo. These RASSLs can be used to activate G(s signaling in any tissue, but would be particularly useful for analyzing downstream events that mediate body weight regulation in mice. Our study also demonstrates the use of human genetic variation for protein engineering.

  15. Loss of Gsα in the Postnatal Skeleton Leads to Low Bone Mass and a Blunted Response to Anabolic Parathyroid Hormone Therapy.

    Science.gov (United States)

    Sinha, Partha; Aarnisalo, Piia; Chubb, Rhiannon; Poulton, Ingrid J; Guo, Jun; Nachtrab, Gregory; Kimura, Takaharu; Swami, Srilatha; Saeed, Hamid; Chen, Min; Weinstein, Lee S; Schipani, Ernestina; Sims, Natalie A; Kronenberg, Henry M; Wu, Joy Y

    2016-01-22

    Parathyroid hormone (PTH) is an important regulator of osteoblast function and is the only anabolic therapy currently approved for treatment of osteoporosis. The PTH receptor (PTH1R) is a G protein-coupled receptor that signals via multiple G proteins including Gsα. Mice expressing a constitutively active mutant PTH1R exhibited a dramatic increase in trabecular bone that was dependent upon expression of Gsα in the osteoblast lineage. Postnatal removal of Gsα in the osteoblast lineage (P-Gsα(OsxKO) mice) yielded markedly reduced trabecular and cortical bone mass. Treatment with anabolic PTH(1-34) (80 μg/kg/day) for 4 weeks failed to increase trabecular bone volume or cortical thickness in male and female P-Gsα(OsxKO) mice. Surprisingly, in both male and female mice, PTH administration significantly increased osteoblast numbers and bone formation rate in both control and P-Gsα(OsxKO) mice. In mice that express a mutated PTH1R that activates adenylyl cyclase and protein kinase A (PKA) via Gsα but not phospholipase C via Gq/11 (D/D mice), PTH significantly enhanced bone formation, indicating that phospholipase C activation is not required for increased bone turnover in response to PTH. Therefore, although the anabolic effect of intermittent PTH treatment on trabecular bone volume is blunted by deletion of Gsα in osteoblasts, PTH can stimulate osteoblast differentiation and bone formation. Together these findings suggest that alternative signaling pathways beyond Gsα and Gq/11 act downstream of PTH on osteoblast differentiation. PMID:26598522

  16. Covariant κ-symmetry gauge fixing and the classical relation between physical variables of the NSR string and the type II GS superstring

    International Nuclear Information System (INIS)

    The main goal of this paper is the manifestly covariant derivation of the classical relation between the gauge-fixed Grassmann variables of the NSR string and the Type II GS superstring. To this end we analyze the superembedding equation for the Type II superstring to derive the relation between the original variables of the NSR string and the Type II GS superstring and, further, by means of Lorentz harmonic variables we fix κ-symmetry of the GS superstring in the manifestly Lorentz covariant way

  17. Covariant $\\kappa-$Symmetry Gauge Fixing and the Classical Relation Between Physical Variables of the NSR String and the Type II GS Superstring

    CERN Document Server

    Uvarov, D V

    2001-01-01

    The main goal of this paper is the manifestly covariant derivation of the classical relation between the gauge-fixed Grassmann variables of the NSR string and the Type II GS superstring. To this end we analyze the superembedding equation for the Type II superstring to derive the relation between the original variables of the NSR string and the Type II GS superstring and, further, by means of Lorentz harmonic variables we fix $\\kappa-$symmetry of the GS superstring in the manifestly Lorentz covariant way.

  18. Implementation of a management system in accordance with IAEA GS-R-3 Standard. A gap analysis

    International Nuclear Information System (INIS)

    Full text: The design and implementation of an Integrated Management System at SNN SA Headquarters become necessary as the CNCAN norms are already under revision to comply with the IAEA GS-R-3 standard. The purpose of this analysis is to draft a project for the transition from a Quality Management System (QMS) to an Integrated Management System (IMS) complying with GS-R-3 requirements. Four steps were identified for developing this project: STEP1 - To justify the necessity of the IMS implementation to meet the SNN SA Headquarters Top Management commitments. The requirements for implementing an IMS are analyzed and a comprehensive document is issued to (and maybe discussed with) SNN General Director in order to obtain the top management adherence/commitment to the project implementation. The document will show the strong and the weak points which should be considered in developing the project. The references for the project are: - IAEA Safety Standard GS-R-3 'The Management System for Facilities and Activities'; - ISO - 1400/2004 Standard 'Environmental Management System Requirements'; - OHSAS 18001/2007 Standard 'Occupational Health and Safety Management Systems. Requirements'; There are also considered: - IAEA Safety Guide GS-G-3.1 'Application of the Management System for Facilities and Activities'; - IAEA Draft Safety Guide DS-349 'Application of the Management System for Nuclear Facilities; There will be considered: Workshop 2 Bookmarks (F5) 2 - CNCAN Norms (as they will be revised); STEP2 - The performance of a comparative analysis of the requirements of GS-R-3, ISO 14001 and OHSAS 18001 versus the provisions of the QMS already implemented in SNN. This analysis is shown as a comparative table; STEP3 - Identification of the IMS processes. An overall analysis of the current processes described in the SNN QMS Manual is performed and based on this. There are identified the additional processes that have to be documented for the proper implementation of an IMS

  19. Our trial to develop a risk assessment tool for CO2 geological storage (GERAS-CO2GS)

    Science.gov (United States)

    Tanaka, A.; Sakamoto, Y.; Komai, T.

    2012-12-01

    We will introduce our researches about to develop a risk assessment tool named 'GERAS-CO2GS' (Geo-environmental Risk Assessment System, CO2 Geological Storage Risk Assessment System) for 'Carbon Dioxide Geological Storage (Geological CCS)'. It aims to facilitate understanding of size of impact of risks related with upper migration of injected CO2. For gaining public recognition about feasibility of Geological CCS, quantitative estimation of risks is essential, to let public knows the level of the risk: whether it is negligible or not. Generally, in preliminary hazard analysis procedure, potential hazards could be identified within Geological CCS's various facilities such as: reservoir, cap rock, upper layers, CO2 injection well, CO2 injection plant and CO2 transport facilities. Among them, hazard of leakage of injected C02 is crucial, because it is the clue to estimate risks around a specific injection plan in terms of safety, environmental protection effect and economy. Our risk assessment tool named GERAS-CO2GS evaluates volume and rate of retention and leakage of injected CO2 in relation with fractures and/or faults, and then it estimates impact of seepages on the surface of the earth. GERAS-CO2GS has four major processing segments: (a) calculation of CO2 retention and leakage volume and rate, (b) data processing of CO2 dispersion on the surface and ambient air, (c) risk data definition and (d) evaluation of risk. Concerning to the injection site, we defined a model, which is consisted from an injection well and a geological strata model: which involves a reservoir, a cap rock, an upper layer, faults, seabed, sea, the surface of the earth and the surface of the sea. For retention rate of each element of CO2 injection site model, we use results of our experimental and numerical studies on CO2 migration within reservoirs and faults with specific lithological conditions. For given CO2 injection rate, GERAS-CO2GS calculates CO2 retention and leakage of each segment

  20. 基于 Roche 454 GS FLX 的钝吻黄盖鲽(Pleuronectes yokohamae)微卫星标记的开发%Microsatellite Loci Isolated by Roche 454 GS FLX in Pleuronectes yokohamae

    Institute of Scientific and Technical Information of China (English)

    潘婷; 张岩; 张辉; 高天翔; 肖永双; 姜云荣

    2015-01-01

    Roche 454 GS FLX, the second-generation sequencing technique, can read longer fragments accurately and isolate SSR markers, and thus has been widely used in many fields of research. In this study, we applied Roche 454 GS FLX to isolate the microsatellite loci and developed the identification of microsatellites markers in Pleuronectes yokohamae. (AC)12, (AG)12, (AAT)12, (AGG)8, (AGC)8, (AGAT)8 and (ACAG)6 were hybridized with shotgun library of DNA samples. This SSR library was constructed through magnetic beads enrichment, cleaning, elution and purification. The SSR library was then sequenced with Roche 454 GS FLX. MISA was used to search for microsatellite motifs and primer 3 was used to design the primers for P. yokohamae. FAM, HEX, and TAMRA were used to label the microsatellite primers (5′), which were used in the triplex PCR or the nested PCR to identify the genotype of the microsatellite loci. The cluster similarity comparison method was first used to analyze 5641 loci that we obtained, and 247 types of loci with high polymorphism were screened. Among these loci, 52.22% were perfect, 20.24% were imperfect and the rest 27.54% were compound. The percentage of (AC)n and (AG)n was 44% in these loci, and 87.5% of them were more than 10-time repeats. Eleven pairs of fluorescent primers were designed according to the selected loci (more than 10-time repeats) and were applied in 5 individuals. The number of alleles per locus ranged from 3 to 8 with an average value of 5. The average values of Ho, He and PIC were 0.588, 0.788 and 0.670 respectively. Four loci among 11 deviated from Hardy-Weinberg. The results of this research indicated that 454 GS FLX was an intuitive and efficient technique for microsatellite isolating. Our study provides essential information about the population genetic diversity of P. yokohamae, and helps improve the artificial breeding.%利用 Roche 454 GS FLX 平台测序技术进行了钝吻黄盖鲽微卫星引物筛选,并采用聚

  1. Highly-linear sampling receivers in silicon and BiCMOS processes for multi-GS/s optically and electrically sampled systems

    OpenAIRE

    Gathman, Timothy D.

    2012-01-01

    In this dissertation, high-linearity sampling circuits are presented for wideband (multi-GS/s) electrical and optical sampling, millimeter-wave circuits are discussed for wideband communication, and injection-locking is presented for nonlinear sampling and spectrum analysis. A 2GS/s sampling receiver with 4.5 measured effective-number-of-bits is presented for spectrally efficient optical communication in a 45nm SOI CMOS process. Next, jitter limitations for broadband sampling circuits are dis...

  2. Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase α-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?

    OpenAIRE

    Man, Huimin; Pollmann, Stephan; Weiler, Elmar W.; Kirby, Edward G.

    2011-01-01

    The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA α-subunit (ASA1) transcript an...

  3. Small Molecule GS-Nitroxide Ameliorates Ionizing Irradiation-Induced Delay in Bone Wound Healing in a Novel Murine Model

    OpenAIRE

    Gokhale, Abhay; Rwigema, Jean-Claude; Epperly, Michael; Glowacki, Julie; Wang, Hong; Wipf, Peter; Goff, Julie P.; Dixon, Tracy; Patrene, Ken; Greenberger, Joel S.

    2010-01-01

    We studied radioprotection and mitigation by mitochondrial-targeted Tempol (GS-nitroxide, JP4-039), in a mouse injury/irradiation model of combined injury (fracture/irradiation). Right hind legs of control C57BL/6NHsd female mice, mice pretreated with MnSOD-PL, JP4-039, or with amifostine were irradiated with single and fractionated doses of 0 to 20 Gy. Twenty-four hours later, unicortical holes were drilled into the tibiae of both hind legs; at intervals, tibias were excised, radiographed, a...

  4. A Mutational Analysis of Residues in Cholera Toxin A1 Necessary for Interaction with Its Substrate, the Stimulatory G Protein Gsα

    Directory of Open Access Journals (Sweden)

    Michael G. Jobling

    2015-03-01

    Full Text Available Pathogenesis of cholera diarrhea requires cholera toxin (CT-mediated adenosine diphosphate (ADP-ribosylation of stimulatory G protein (Gsα in enterocytes. CT is an AB5 toxin with an inactive CTA1 domain linked via CTA2 to a pentameric receptor-binding B subunit. Allosterically activated CTA1 fragment in complex with NAD+ and GTP-bound ADP-ribosylation factor 6 (ARF6-GTP differs conformationally from the CTA1 domain in holotoxin. A surface-exposed knob and a short α-helix (formed, respectively, by rearranging “active-site” and “activation” loops in inactive CTA1 and an ADP ribosylating turn-turn (ARTT motif, all located near the CTA1 catalytic site, were evaluated for possible roles in recognizing Gsα. CT variants with one, two or three alanine substitutions at surface-exposed residues within these CTA1 motifs were tested for assembly into holotoxin and ADP-ribosylating activity against Gsα and diethylamino-(benzylidineamino-guanidine (DEABAG, a small substrate predicted to fit into the CTA1 active site. Variants with single alanine substitutions at H55, R67, L71, S78, or D109 had nearly wild-type activity with DEABAG but significantly decreased activity with Gsα, suggesting that the corresponding residues in native CTA1 participate in recognizing Gsα. As several variants with multiple substitutions at these positions retained partial activity against Gsα, other residues in CTA1 likely also participate in recognizing Gsα.

  5. The Populus superoxide dismutase gene family and its responses to drought stress in transgenic poplar overexpressing a pine cytosolic glutamine synthetase (GS1a.

    Directory of Open Access Journals (Sweden)

    Juan Jesús Molina-Rueda

    Full Text Available BACKGROUND: Glutamine synthetase (GS plays a central role in plant nitrogen assimilation, a process intimately linked to soil water availability. We previously showed that hybrid poplar (Populus tremula X alba, INRA 717-1B4 expressing ectopically a pine cytosolic glutamine synthetase gene (GS1a display enhanced tolerance to drought. Preliminary transcriptome profiling revealed that during drought, members of the superoxide dismutase (SOD family were reciprocally regulated in GS poplar when compared with the wild-type control, in all tissues examined. SOD was the only gene family found to exhibit such patterns. RESULTS: In silico analysis of the Populus genome identified 12 SOD genes and two genes encoding copper chaperones for SOD (CCSs. The poplar SODs form three phylogenetic clusters in accordance with their distinct metal co-factor requirements and gene structure. Nearly all poplar SODs and CCSs are present in duplicate derived from whole genome duplication, in sharp contrast to their predominantly single-copy Arabidopsis orthologs. Drought stress triggered plant-wide down-regulation of the plastidic copper SODs (CSDs, with concomitant up-regulation of plastidic iron SODs (FSDs in GS poplar relative to the wild type; this was confirmed at the activity level. We also found evidence for coordinated down-regulation of other copper proteins, including plastidic CCSs and polyphenol oxidases, in GS poplar under drought conditions. CONCLUSIONS: Both gene duplication and expression divergence have contributed to the expansion and transcriptional diversity of the Populus SOD/CCS families. Coordinated down-regulation of major copper proteins in drought-tolerant GS poplars supports the copper cofactor economy model where copper supply is preferentially allocated for plastocyanins to sustain photosynthesis during drought. Our results also extend previous findings on the compensatory regulation between chloroplastic CSDs and FSDs, and suggest that this

  6. Modelling LAI at a regional scale with ISBA-A-gs: comparison with satellite-derived LAI over southwestern France

    Directory of Open Access Journals (Sweden)

    A. Brut

    2009-04-01

    Full Text Available A CO2-responsive land surface model (the ISBA-A-gs model of Météo-France is used to simulate photosynthesis and Leaf Area Index (LAI in southwestern France for a 3-year period (2001–2003. A domain of about 170 000 km2 is covered at a spatial resolution of 8 km. The capability of ISBA-A-gs to reproduce the seasonal and the inter-annual variability of LAI at a regional scale, is assessed with two satellite-derived LAI products. One originates from the CYCLOPES programme using SPOT/VEGETATION data, and the second is based on MODIS data. The comparison reveals discrepancies between the two satellite LAI estimates and between satellite and simulated LAI values, both in their intensity and in the timing of the leaf onset. The model simulates higher LAI values for the C3 crops and coniferous trees than the satellite observations, which may be due to a saturation effect within the satellite signal. The simulated leaf onset presents a significant delay for mountainous grasslands. In-situ observations at a mid-altitude grassland site show that the generic temperature response of photosynthesis used in the model is not appropriate for plants adapted to the cold climatic conditions of the mountainous areas. This study demonstrates the potential of LAI remote sensing products for identifying and locating models' shortcomings at a regional scale.

  7. Assimilation of Cyclopes Leaf Area Index into the ISBA-A-GS Land Surface Model over France

    Science.gov (United States)

    Barbu, Alina L.; Calvet, J.-C.; Lafont, S.

    2011-01-01

    In our study the SURFEX modeling platform is used in off-line mode. It contains the land surface model ISBA- A-gs that simulates photosynthesis and plant growth. A version of the Extended Kalman Filter scheme is used for the assimilation in situ and CYCLOPES LAI product de- rived from the SPOT4/VEGETATION sensor within the ISBA-A-gs model. The LAI data is incorporated into the model every 10 days to analyse its impact on vegetation biomass. The experiment is performed over France at a spatial resolution of 8 km. A model grid box is divided in a number of patches each having its own set of prognostic variables. The filter algorithm is designed to provide the analysis for each patches independently by using one observation per grid box. When needed, the updated values are aggregated by computing a weighted average. In this study we demonstrate that the assimilation scheme works effectively within the multi-patch version of our model. An important reduction of the LAI bias between the model and measurements is achieved.

  8. A 4.2 GS/s Synchronized Vertical Excitation System for SPS Studies - Steps Toward Wideband Feedback

    CERN Document Server

    Fox, JD; Rivetta, C; Rivetta, I; Turgut, O; Uemura, S; Hofle, W; Wehrle, U.

    2012-01-01

    A 4.2 GS/sec. beam excitation system with accelerator synchronization and power stages is described. The system is capable of playing unique samples (32 samples/bunch) for 15,000 turns on selected bunch(es) in the SPS in synchronism with the injection and acceleration cycle. The purpose of the system is to excite internal modes of singlebunch vertical motion, and study the bunch dynamics in the presence of developing Electron cloud or TMCI effects. The system includes a synchronized master oscillator, SPS timing functions, an FPGA based arbitrary waveform generator, 4.2 GS/sec. D/A system and four 80W 20 -1000 MHz amplifiers driving a tapered stripline pickup/kicker. A software GUI allows specification of various modulation signals, selection of bunches and turns to excite, while a remote control interface allows simple control/monitoring of the RF power stages located in the tunnel. The successful use of this system for SPS MD measurements in 2011 is a vital proof-of-principle for wideband feedback using s...

  9. A 4.2 GS/sec. Synchronized Vertical Excitation System for SPS Studies - Steps Toward Wideband Feedback

    International Nuclear Information System (INIS)

    A 4.2 GS/sec. beam excitation system with accelerator synchronization and power stages is described. The system is capable of playing unique samples (32 samples/bunch) for 15,000 turns on selected bunch(es) in the SPS in syn- chronism with the injection and acceleration cycle. The purpose of the system is to excite internal modes of single-bunch vertical motion, and study the bunch dynamics in the presence of developing Electron cloud or TMCI effects. The system includes a synchronized master oscillator, SPS timing functions, an FPGA based arbitrary waveform generator, 4.2 GS/sec. D/A system and four 80W 20-1000 MHz amplifiers driving a tapered stripline pickup/kicker. A software GUI allows specification of various modulation signals, selection of bunches and turns to excite, while a remote control interface allows simple control/monitoring of the RF power stages located in the tunnel. The successful use of this system for SPS MD measurements in 2011 is a vital proof-of-principle for wideband feedback using similar functions to correct the beam motion.

  10. A combined coalescence gene-dropping tool for evaluating genomic selection in complex scenarios (ms2gs).

    Science.gov (United States)

    Pérez-Enciso, M; Legarra, A

    2016-04-01

    We present ms2gs, a combined coalescence - gene dropping (i.e. backward-forward) simulator for complex traits. It therefore aims at combining the advantages of both approaches. It is primarily conceived for very short term, recent scenarios such as those that are of interest in animal and plant breeding. It is very flexible in terms of defining QTL architecture and SNP ascertainment bias, and it allows for easy modelling of alternative markers such as RADs. It can use real sequence or chip data or generate molecular polymorphisms via the coalescence. It can generate QTL conditional on extant molecular information, such as low-density genotyping. It models (simplistically) sequence, imputation or genotyping errors. It requires as input both genotypic data in plink or ms formats, and a pedigree that is used to perform the gene dropping. By default, it compares accuracy for BLUP, SNP ascertained data, sequence, and causal SNPs. It employs VanRaden's linear (GBLUP) and nonlinear method for incorporating molecular information. To illustrate the program, we present a small application in a half-sib population and a multiparental (MAGIC) cross. The program, manual and examples are available at https://github.com/mperezenciso/ms2gs. PMID:26995218

  11. Enhanced expression of glutamine synthetase (GS1a) confers altered fibre and wood chemistry in field grown hybrid poplar (Populus tremula X alba) (717-1B4).

    Science.gov (United States)

    Coleman, Heather D; Cánovas, Francisco M; Man, Huimin; Kirby, Edward G; Mansfield, Shawn D

    2012-09-01

    Hybrid poplar (Populus tremula X P. alba) genetically engineered to express the pine cytosolic glutamine synthetase gene (GS1a) has been previously shown to display desirable field performance characteristics, including enhancements in growth and nitrogen use efficiency. Analysis of wood samples from a 3-year-old field trial of three independently transformed GS1a transgenic hybrid poplar lines revealed that, when compared with wild-type controls, ectopic expression of GS1a resulted in alterations in wood properties and wood chemistry. Included were significant enhancements in wood fibre length, wood density, microfibre angle, per cent syringyl lignin and elevated concentrations of wood sugars, specifically glucose, galactose, mannose and xylose. Total extractive content and acid-insoluble lignin were significantly reduced in wood of GS1a transgenics when compared with wild-type trees. Together, these cell wall characteristics resulted in improved wood pulping attributes, including improved lignin solubilization with no concurrent decrease in yield. Trees with increased GS1a expression have improved characteristics for pulp and paper production and hold potential as a feedstock for biofuels production. PMID:22672155

  12. Enhancement of humoral immunity in mice by coupling pUCpGs10 and aluminium to the HCV recombinant immunogen

    Directory of Open Access Journals (Sweden)

    Zhan Na

    2011-11-01

    Full Text Available Abstract Aim To investigate the enhancement of humoral immunity when CpG ODN (cytidine phosphate guanosine oligodeoxynucleotides and aluminium adjuvants are complexed with the HCV (Hepatitis C virus recombinant immunogen in mice. Methods After immunizing Balb/c mice with the recombination HCV antigen adjuvanted with pUCpGs10 and/or aluminium(antigen+CpG+alum, antigen+CpG, antigen+alum, antigen+PBS, enzyme-linked immunosorbent assay (ELISA was used to measure the specific serum antibody titers of IgG, to determine the neutralization response to various peptide genotypes, and to determine the concentration of IL-6 and IL-10 in supernatants of in vitro cultured splenic lymphocytes. Enzyme-linked immunospot assay (ELISPOT was used to quantify the non-specific and specific splenic antibody-secreting cells (ASCs, and flow cytometry (FCM determined the ratio of different splenic lymphocytes. The serum of rabbits immunized with the recombinant pBVGST/HVR1 antigen immunoprecipitated the HCV isolated from 12 patients' serum. Results The sera antibody titers were 1:51200, 1:9051, 1:18102, 1:6400 respectively after the final immunization and demonstrated good neutralization responses to the six gene peptide containing 1a, 1b, 2a, 3a, 4a and 6a. The aluminum adjuvant increased the population of both specific ASCs (P +CD27+ (P +CD38+ splenic lymphocytes with the aluminum and pUCpGs10 adjuvant present compared to the control group(P Conclusions 1. The aluminum adjuvant induces a potent Th2-biased immune response by increasing both the populations of specific and total ASCs and the ratio of CD19+CD27+ cells. 2. The pUCpGs10 complexed with the aluminum adjuvant boosts the population of plasma cells and increase the efficiency of the immune response. 3. The two adjuvants have synergistic effects on humoral immunity. 4. The recombinant HVR1 protein has the possibility of generating broadly reactive anti-HVR1 antibody.

  13. Appraisement on Contributive Ratio of Scientific and Technical Progresses in Milk Productive Enterprises by Model C2GS2

    Institute of Scientific and Technical Information of China (English)

    SUN Futian; SUN Liqun; YANG Guanglin

    2008-01-01

    Scientific and technical progress has been the driving forces of enterprises development. Milk productive enterprises are developing faster and growing better. It is very important to measure the contributive ratio of scientific and technical progress in milk productive enterprises. And the appraisement could help to develop milk productive enterprises. The model C2GS2 was established to appraise the contributive ratio of scientific and technical progress in milk productive enterprises in the research. And the appraisement on the contributive ratio of scientific and technical progress in milk productive enterprises was made by the model. In the results of appraisement, science and technology play a main role in milk productive enterprises. It is shown that our milk productive enterprises are developed by scientific and technical progress while not by input of productive factors.

  14. Bimodal T_gs and Macro- Phase Separation of As_2-xGe_xS3 Glasses^*

    Science.gov (United States)

    Mamedov, Sergey; Georgiev, Daniel; Qu, Tao; Boolchand, Punit

    2003-03-01

    Fifteen glass compositions in the titled ternary have been synthesized, and examined in MDSC [1] and Raman scattering measurements. Single T_gs appear in the 0 xt = 1.6. The latter results from ethanelike Ge_2S3 nanophase that segregates [1, 2] from the GeS_m-rich phase as m xt in region III. Raman scattering shows narrow modes of As_4S4 and As_4S3 units that apparently cluster to form the AsSn -rich macrophase in region II. The present glasses, in contrast to their Selenide counterparts, are heterogeneous on a macroscopic scale in spite of attempts to homogenize melts by periodic shaking for extended periods. ^* Supported by NSF grant DMR 01-010808 [1] L.Cai and P.Boolchand Phil. Mag B 82, 1649(2002). [2] P.Boolchand et al. Solid State Commun. 45,183(1983)

  15. Carbon steel protection in G.S. (Girlder sulfide) plants. Pressure influence on iron sulfide scales formation. Pt. 5

    International Nuclear Information System (INIS)

    In order to protect carbon steel towers and piping of Girlder sulfide (G.S.) experimental heavy water plants against corrosion produced by the action of aqueous solutions of hydrogen sulfide, a method, previously published, was developed. Carbon steel, exposed to saturated aqueous solutions of hydrogen sulfide, forms iron sulfide scales. In oxygen free solutions evolution of corrosion follows the sequence: mackinawite → cubic ferrous sulfide → troilite → pyrrotite → pyrite. Scales formed by pyrrotite-pyrite or pyrite are the most protective layers (these are obtained at 130 deg C, 2MPa, for periods of 14 days). Experiments, at 125 deg C and periods of 10-25 days, were performed in two different ways: 1- constant pressure operations at 0.5 and 1.1 MPa. 2- variable pressure operation between 0.3-1 MPa. In all cases pyrrotite-pyrite scales were obtained. (Author)

  16. Radiographic findings and Gs-alpha bioactivity studies and mutation screening in acrodysostosis indicate a different etiology from pseudohypoparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Graham, J.M. Jr.; Krakow, D.; Smith, A.K.; Lachman, R.S. [UCLA, Los Angeles, CA (United States). School of Medicine; Tolo, V.T. [Div. of Orthopedics, Children' s Hospital of Los Angeles, CA (United States)

    2001-01-01

    Acrodysostosis is an uncommon skeletal dysplasia associated with nasal hypoplasia, midface deficiency, severe brachydactyly, and varying degrees of hearing loss and mental retardation. Previous publications have suggested that it may be difficult to distinguish acrodystostosis from pseudohypoparathyroidism on clinical grounds, but acrodysostosis does appear to have distinct clinical and radiologic findings. Spinal stenosis is an underappreciated risk in acrodysostosis, despite the reported loss of normal caudal widening of the lumbar interpediculate distance on AP spine radiographs in the original report of this disorder by Robinow et al., with confirmation of these radiographic findings by Butler et al. We report two sporadic cases of acrodysostosis, one of which required decompressive laminectomy for symptomatic spinal stenosis, and review 11 cases of acrodysostosis from 9 families that were submitted to the International Skeletal Dysplasia Registry. The objective of this report is to determine the frequency and severity of spinal stenosis in patients with acrodysostosis and to summarize the clinical and radiographic findings of acrodysostosis in an effort to distinguish acrodysostosis clearly from pseudohypoparathyroidism. The pattern of brachydactyly differs between these two conditions, and varying degrees of spinal stenosis are characteristic of acrodysostosis. Both our index patients with acrodysostosis had normal bioactivity of the alpha subunit of the Gs protein, therefore indicating that acrodysostosis has a different pathogenesis from pseudohypoparathyroidism. Furthermore, single-strand confirmational polymorphism (SSCP) analysis failed to demonstrate any confirmational alterations in the coding exons of the Gs alpha gene. These radiographic and laboratory findings substantiate that acrodysostosis is clinically different from pseudohypoparathyroidism and that it is necessary to follow patients with acrodysostosis for signs of spinal stenosis. (orig.)

  17. Radiographic findings and Gs-alpha bioactivity studies and mutation screening in acrodysostosis indicate a different etiology from pseudohypoparathyroidism

    International Nuclear Information System (INIS)

    Acrodysostosis is an uncommon skeletal dysplasia associated with nasal hypoplasia, midface deficiency, severe brachydactyly, and varying degrees of hearing loss and mental retardation. Previous publications have suggested that it may be difficult to distinguish acrodystostosis from pseudohypoparathyroidism on clinical grounds, but acrodysostosis does appear to have distinct clinical and radiologic findings. Spinal stenosis is an underappreciated risk in acrodysostosis, despite the reported loss of normal caudal widening of the lumbar interpediculate distance on AP spine radiographs in the original report of this disorder by Robinow et al., with confirmation of these radiographic findings by Butler et al. We report two sporadic cases of acrodysostosis, one of which required decompressive laminectomy for symptomatic spinal stenosis, and review 11 cases of acrodysostosis from 9 families that were submitted to the International Skeletal Dysplasia Registry. The objective of this report is to determine the frequency and severity of spinal stenosis in patients with acrodysostosis and to summarize the clinical and radiographic findings of acrodysostosis in an effort to distinguish acrodysostosis clearly from pseudohypoparathyroidism. The pattern of brachydactyly differs between these two conditions, and varying degrees of spinal stenosis are characteristic of acrodysostosis. Both our index patients with acrodysostosis had normal bioactivity of the alpha subunit of the Gs protein, therefore indicating that acrodysostosis has a different pathogenesis from pseudohypoparathyroidism. Furthermore, single-strand confirmational polymorphism (SSCP) analysis failed to demonstrate any confirmational alterations in the coding exons of the Gs alpha gene. These radiographic and laboratory findings substantiate that acrodysostosis is clinically different from pseudohypoparathyroidism and that it is necessary to follow patients with acrodysostosis for signs of spinal stenosis. (orig.)

  18. Production of a monoclonal antibody specific for high molecular weight glutenin subunits (HMW-GS) in wheat and its antigenic determinant

    Institute of Scientific and Technical Information of China (English)

    WANG Hanqian; ZHANG Xueyong; WANG Hongmei; PANG Binshuang

    2005-01-01

    Wheat high molecular weight glutenin subunits (HMW-GS) 1Bx14 and 1By15 isolated by preparative SDS-PAGE are used as antigen to immunize BALB/c mice. Subcutaneous inoculation of the antigen is performed. The intra-peritoneal injection is completed 3 days before fusion with myeloma cell (SP2/0) via PEG-1500. The fusion cells are selected by indirect enzyme-linked immuno-sorbent assay (ELISA). Positive hybrid cells are further verified three times by limit dilution of the culture cells. A hybridoma cell line is successfully obtained. The monoclonal antibody belongs to IgG1 subclass. In immunoblotting, the antibody binds to all HMW-GS of T.aestivum cultivars, but does not bind to other storage proteins in seeds of wheat. This result is consisting with the high homology in amino acid sequences among the HMW glutenin subunits in wheat. The antibody also binds to HMW-GS storage proteins in Aegilops squarrosa and T. durum (durum wheat). Furthermore, it also binds to HMW storage proteins in Secale cereale (rye),Hordeum vulgare (barley). However, it never binds seed storage proteins in other cereals such as maize, oat, rice, foxtail millet, sorghum etc. The antigen determinant recognized by the antibody has been located within hexapeptide [PGQGQQ] or / and nonapeptide [GYYPTSPQQ] in the central repetitive region of HMW-GS.

  19. Molecular characterization of LMW-GS genes from a somatic hybrid introgression line Ⅱ-12 between Triticum aestivum and Agropyron elongatum in relation to quick evolution

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In order to exploit the evolution and find novel low-molecular-weight glutenin subunit(LMW-GS)for improvement of common wheat quality,thirteen variants from a somatic hybrid introgression line Ⅱ-12 between Triticum aestivum cv.Jinan 177(JN177)and Agropyron elongatum were characterized via genomic PCR.Four clones were pseudogenes because they contained an internal stop codon.The remaining nine variants contained intact open reading frames(ORFs).Sequence alignment indicates that the proteins deduced from the nine ORFs have similar primary structure with LMW-GS cloned from its parents previously.However,they have some unique modifications in the structures.For example,EU292737 contains not only an extra Cys residue in the C-terminal domain but also a long repetitive domain.Both EU159511 and EU292738 start their first Cys residue in the N-terminal repetitive domain,but not in the N-conserved domain traditionally.These structural alterations may have positive contributions to wheat flour quality.The results of phylogeny showed that most LMW-GS variances from Ⅱ-12 were homologous to those from parent JN 177 and other wheat lines.The reason for quick evolution of LMW-GS in Ⅱ-12 was discussed.

  20. A Novel T55A Variant of Gsα Associated with Impaired cAMP Production, Bone Fragility, and Osteolysis

    Directory of Open Access Journals (Sweden)

    Kelly Wentworth

    2016-01-01

    Full Text Available G-protein coupled receptors (GPCRs mediate a wide spectrum of biological activities. The GNAS complex locus encodes the stimulatory alpha subunit of the guanine nucleotide binding protein (Gsα and regulates production of the second messenger cyclic AMP (cAMP. Loss-of-function GNAS mutations classically lead to Albright’s Hereditary Osteodystrophy (AHO and pseudohypoparathyroidism, often with significant effects on bone formation and mineral metabolism. We present the case of a child who exhibits clinical features of osteolysis, multiple childhood fractures, and neonatal SIADH. Exome sequencing revealed a novel de novo heterozygous missense mutation of GNAS (c.163AGsα GTPase domain. In order to further assess whether this unique mutation resulted in a gain or loss of function of Gsα, we introduced the mutation into a rat GNAS plasmid and performed functional studies to assess the level of cAMP activity associated with this mutation. We identified a 64% decrease in isoproterenol-induced cAMP production in vitro, compared to wild type, consistent with loss of Gsα activity. Despite a significant decrease in isoproterenol-induced cAMP production in vitro, this mutation did not produce a classical AHO phenotype in our patient; however, it may account for her presentation with childhood fractures and osteolysis.

  1. Inhibition of low- and high-threshold Ca2+ channels of human neuroblastoma IMR32 cells by Lambert-Eaton myasthenic syndrome (LEMS) IgGs.

    Science.gov (United States)

    Grassi, C; Magnelli, V; Carabelli, V; Sher, E; Carbone, E

    1994-11-01

    IgGs from two LEMS patients applied to human neuroblastoma IMR32 cells reduced the density of low- (LVA; T) and high-threshold (HVA; L and N) Ba2+ currents by different percentages: 36% (LVA) and 56% (HVA) for one and 48% and 45% for the other. A pharmacological assay of IgGs action based on the block of L-type channel by nifedipine and on the delayed activation of N-type channel by noradrenaline, indicated a preferential inhibition of the N-type current in IMR32 cells (55% and 47% for the two patients). The L-type current, contributing to approximately one-third of the total, was also depressed by LEMS IgGs but to a minor degree (49% and 30%). Except for an increase of single N-type channel inactivation, LEMS antibodies preserved the elementary properties of single HVA channels, suggesting that the macroscopic current reduction after IgGs treatment is likely due to a decrease in the number of active HVA Ca2+ channels. PMID:7898770

  2. Mutations in Adenosine Deaminase-like (ADAL) Protein Confer Resistance to the Antiproliferative Agents N-6-Cyclopropyl-PMEDAP and GS-9219

    Czech Academy of Sciences Publication Activity Database

    Frey, C. R.; Andrei, G.; Votruba, Ivan; Cannizzaro, C.; Han, B.; Fung, W. C.; Hung, M.; Liu, X. H.; Geleziunas, R.; Fiten, P.; Opdenakker, G.; Snoeck, R.; Cihlar, T.

    2013-01-01

    Roč. 33, č. 5 (2013), s. 1899-1912. ISSN 0250-7005 Grant ostatní: K.U.Leuven(BE) PF/10/018 Institutional support: RVO:61388963 Keywords : ADAL * deamination * GS-9219 * PMEG Subject RIV: CE - Biochemistry Impact factor: 1.872, year: 2013

  3. Analysis and Extracting Method for DVB-S2 GS%DVB-S2 GS流的分析与提取方法

    Institute of Scientific and Technical Information of China (English)

    邵永庆; 黄焱; 王翔宇

    2013-01-01

    随着卫星技术与互联网技术的进一步融合,通过数字广播电视(DVB-S)信道传输的业务种类大幅增加。第一代系统难以应付快速增长的IP数据业务,为此,第二代数字卫星广播标准(DVB-S2)制定一种新的码流:一般传输流(GS)。针对现有卫星设备缺乏对GS流处理的现状,根据基带帧自身的格式、GS流在基带帧中的编排方式及GS流专用封装一般传输流封装(GSE)的特点,提出一种DVB-S2 GS流分析与提取的方法,对基带帧的封装方式及GS流的封装GSE进行分析,并用实际数据进行测试。实验结果证明,该方法能够快速准确地还原出应用层中的IP数据,实现对GS流的处理。%Along with the combination of satellite and Internet technology, business lines transmitted by satellite Digital Video Broadcasting-Satellite(DVB-S) channel increases rapidly. The first generation system can not meet the need of the fast increasing IP business, hence, an entirely new Generic Stream(GS) is introduced in DVB-S2 standard. Aiming at the problem that the existing satellite terminal equipment can not process GS very well, this paper presents a DVB-S2 GS extraction and analysis method according to the form of generic stream, the arrangement method of GS in base-band frame and feature of Generic Stream Encapsulation(GSE). It analyses the encapsulation mode of base-band frame and GSE of GS, and tests some actual data. Experimental result shows the method can restore IP data in application layer rapidly and accurately, and can realize the processing of GS.

  4. The response of GS-NS0 myeloma cells to single and multiple pH perturbations.

    Science.gov (United States)

    Osman, Jason J; Birch, John; Varley, Julie

    2002-08-20

    Animal cells are cultured in several types of vessels at laboratory and industrial scale the most common being the stirred tank and the air-lift. Economically, it is preferable to culture animal cells at the largest possible scale but the perceived sensitivity of animal cells to hydrodynamic shear has, until now, limited the aeration and agitation rates used. This has been reported to cause inhomogeneities in operational parameters such as dissolved oxygen concentration, temperature and pH. pH is of special interest during the latter stages of many animal cell fermentation because alkali additions, used for pH control, can cause large local pH perturbations of varying size and duration. The effect of single and multiple pH perturbations on the cell growth of a widely used GS-NS0 mouse myeloma cell line grown in batch culture was investigated. The effect of perturbation amplitude and duration was investigated using a single stirred tank reactor (STR). In the single STR system cells were subjected to one pH 8.0 or 9.0 perturbation ranging in duration from 0-90 minutes. No measurable decrease in viable cell number was seen for pH 8.0 perturbations of any duration whereas pH 9.0 perturbations lasting for 10 minutes caused a 15% decrease in viable cell number. The proportion of viable cells decreased with increasing perturbation time and a 90-minute exposure killed all of the cells. The effect of multiple pH perturbations on GS-NS0 cells was investigated using two connected STR's. More specifically the number of perturbations and the perturbation frequency were investigated. Cells were subjected to between 0 and 100 perturbations at pH 8.0; the time between each perturbation (frequency) was 6 minutes and each perturbation lasted for 200 seconds. Viable cell number decreased with increasing perturbation number, with 100 perturbations causing death of 27.5% of cells. Cells were also exposed to 10 perturbations at pH 9.0, each of 200 second duration at frequencies of

  5. The mechano-gated channel inhibitor GsMTx4 reduces the exercise pressor reflex in rats with ligated femoral arteries.

    Science.gov (United States)

    Copp, Steven W; Kim, Joyce S; Ruiz-Velasco, Victor; Kaufman, Marc P

    2016-05-01

    Mechanical and metabolic stimuli arising from contracting muscles evoke the exercise pressor reflex. This reflex is greater in a rat model of simulated peripheral arterial disease in which a femoral artery is chronically ligated than it is in rats with freely perfused femoral arteries. The role played by the mechanically sensitive component of the exaggerated exercise pressor reflex in ligated rats is unknown. We tested the hypothesis that the mechano-gated channel inhibitor GsMTx4, a relatively selective inhibitor of mechano-gated Piezo channels, reduces the exercise pressor reflex in decerebrate rats with ligated femoral arteries. Injection of 10 μg of GsMTx4 into the arterial supply of the hindlimb reduced the pressor response to Achilles tendon stretch (a purely mechanical stimulus) but had no effect on the pressor responses to intra-arterial injection of α,β-methylene ATP or lactic acid (purely metabolic stimuli). Moreover, injection of 10 μg of GsMTx4 into the arterial supply of the hindlimb reduced both the integrated pressor area (control 535 ± 21, GsMTx4 218 ± 24 mmHg·s; P nerve responses to electrically induced intermittent hindlimb muscle contraction (a mixed mechanical and metabolic stimulus). The reduction of the integrated pressor area during contraction caused by GsMTx4 was greater in rats with ligated femoral arteries than it was in rats with freely perfused femoral arteries. We conclude that the mechanically sensitive component of the reflex contributes to the exaggerated exercise pressor reflex during intermittent hindlimb muscle contractions in rats with ligated femoral arteries. PMID:26921442

  6. A "high-hard" outburst of the black hole X-ray binary GS 1354-64

    Science.gov (United States)

    Koljonen, Karri; Russell, David; Corral-Santana, Jesus; Armas Padilla, Montserrat; Munoz-Darias, Teo; Lewis, Fraser

    2016-06-01

    In the shadows of the V404 Cyg outburst in the summer of 2015, GS 1354-64 (BW Cir) went into outburst as well. We followed the evolution of the outburst at optical, UV and X-ray wavelengths using Faulkes Telescope South, SMARTS and Swift. The outburst was found to stay in the hard X-ray state, albeit being anomalously luminous with a peak X-ray luminosity exceeding 0.15 LEdd, which could be the most luminous hard state observed in a black hole X-ray binary. In this talk I will present our results showing that the the outburst evolution at all wavelengths can be explained by the disk instability model with irradiation and disk evaporation/condensation. In addition, I will present our long-term optical monitoring results that show a statistically significant, slow rise of the source brightness over the 7 years prior to the 2015 outburst. This could be the much-sought observational evidence of matter slowly accumulating in the accretion disk, and subsequently getting optically brighter, as predicted by the disk instability model.

  7. A `high-hard' outburst of the black hole X-ray binary GS 1354-64

    Science.gov (United States)

    Koljonen, K. I. I.; Russell, D. M.; Corral-Santana, J. M.; Armas Padilla, M.; Muñoz-Darias, T.; Lewis, F.; Coriat, M.; Bauer, F. E.

    2016-07-01

    We study in detail the evolution of the 2015 outburst of GS 1354-64 (BW Cir) at optical, UV and X-ray wavelengths using Faulkes Telescope South/Las Cumbres Observatory Global Telescope Network, Small & Moderate Aperture Research Telescope System and Swift. The outburst was found to stay in the hard X-ray state, albeit being anomalously luminous with a peak luminosity of LX > 0.15 LEdd, which could be the most luminous hard state observed in a black hole X-ray binary. We found that the optical/UV emission is tightly correlated with the X-ray emission, consistent with accretion disc irradiation and/or a jet producing the optical emission. The X-ray spectra can be fitted well with a Comptonization model, and show softening towards the end of the outburst. In addition, we detect a QPO in the X-ray light curves with increasing centroid frequency during the peak and decay periods of the outburst. The long-term optical light curves during quiescence show a statistically significant, slow rise of the source brightness over the 7 years prior to the 2015 outburst. This behaviour as well as the outburst evolution at all wavelengths studied can be explained by the disc instability model with irradiation and disc evaporation/condensation.

  8. A "high-hard" outburst of the black hole X-ray binary GS 1354-64

    CERN Document Server

    Koljonen, K I I; Corral-Santana, J M; Padilla, M Armas; Muñoz-Darias, T; Lewis, F; Coriat, M; Bauer, F E

    2016-01-01

    We study in detail the evolution of the 2015 outburst of GS 1354-64 (BW Cir) at optical, UV and X-ray wavelengths using Faulkes Telescope South, SMARTS and Swift. The outburst was found to stay in the hard X-ray state, albeit being anomalously luminous with a peak luminosity of L$_{X} >$ 0.15 L$_{Edd}$, which could be the most luminous hard state observed in a black hole X-ray binary. We found that the optical/UV emission is tightly correlated with the X-ray emission, consistent with accretion disc irradiation and/or a jet producing the optical emission. The X-ray spectra can be fitted well with a Comptonisation model, and show softening towards the end of the outburst. In addition, we detect a QPO in the X-ray lightcurves with increasing centroid frequency during the peak and decay periods of the outburst. The long-term optical lightcurves during quiescence show a statistically significant, slow rise of the source brightness over the 7 years prior to the 2015 outburst. This behaviour as well as the outburst ...

  9. Design, fabrication, commissioning, and testing of a 250 g/s, 2-K helium cold compressor system

    International Nuclear Information System (INIS)

    In June 1999 the Thomas Jefferson National Accelerator Facility (TJNAF) Cryogenic Systems Group had completed the design, fabrication, and commissioning of a cold compressor system capable of pumping 250 g/s of 2-K helium vapor to a pressure above 1 bar. The 2-K cold box consists of five stages of centrifugal variable speed compressors with LN2 cooled drive motors and magnetic bearings, a plate fin heat exchanger, and an LN2 shield system. The new 2-K cold box (referred to as the SCN) was built as a redundant system to an existing four stage cold compressor SCM cold box that was commissioned in May 1994. The SCN has been in continuous service supporting the facility experiments since commissioning. This system has achieved a significant improvement in the total 2-K refrigeration system capacity and stability and has substantially increased the operating envelope both in cold compressor flow and operating pressure range. This paper describes the cold box configuration and the experience s in the design, fabrication, commissioning and performance evaluation. The capacity of the system for various operating pressures (0.040 to 0.025 bar at the load corresponding to a total compressor pressure ratio of 28 to 54) is presented. An effort is made to characterize the components and their operating data over the tested range. This includes the return side pressure drop in the distribution system, the heat exchanger, and the cold compressor characteristics. The system design parameters and their effects on performance are outlined

  10. Warm isostatic pressing (WIP'ing) of GS44 Si3N4 FDC parts for defect removal

    International Nuclear Information System (INIS)

    Fused deposition of ceramics (FDC) is one of the developing solid freeform fabrication (SFF) techniques. The successful production of high performance ceramics by the FDC process requires that no defects exist in the green parts. However, build defects, such as missing roads, poorly bonded layers or sub-perimeter voids can be encountered in improperly built FDC parts. In this study, a method known as WIP'ing (warm isostatic pressing) was evaluated for its ability to eliminate existing defects in GS44 Si3N4 green FDC parts. Analogous to CIP'ing (cold isostatic pressing), the green FDC parts were rubber bagged and loaded into a pressure chamber filled with water soluble oil at different temperatures, ranging from 30 to 90 deg. C, at pressures of up to 35 MPa. X-Ray radiography results indicated that at temperatures above 70 deg. C, WIP'ing was effective in closing the gaps of the intentionally placed void defects in FDC parts. However, WIP'ing above 70 deg. C was not effective in healing the defects completely. The fracture strengths of FDC parts with intentional added defects, WIP'ed above 70 deg. C were substantially lower than control samples

  11. A "high-hard" outburst of the black hole X-ray binary GS 1354-64

    Science.gov (United States)

    Koljonen, K. I. I.; Russell, D. M.; Corral-Santana, J. M.; Armas Padilla, M.; Muñoz-Darias, T.; Lewis, F.; Coriat, M.; Bauer, F. E.

    2016-04-01

    We study in detail the evolution of the 2015 outburst of GS 1354-64 (BW Cir) at optical, UV and X-ray wavelengths using Faulkes Telescope South/LCOGT, SMARTS and Swift. The outburst was found to stay in the hard X-ray state, albeit being anomalously luminous with a peak luminosity of LX > 0.15 LEdd, which could be the most luminous hard state observed in a black hole X-ray binary. We found that the optical/UV emission is tightly correlated with the X-ray emission, consistent with accretion disc irradiation and/or a jet producing the optical emission. The X-ray spectra can be fitted well with a Comptonisation model, and show softening towards the end of the outburst. In addition, we detect a QPO in the X-ray lightcurves with increasing centroid frequency during the peak and decay periods of the outburst. The long-term optical lightcurves during quiescence show a statistically significant, slow rise of the source brightness over the 7 years prior to the 2015 outburst. This behaviour as well as the outburst evolution at all wavelengths studied can be explained by the disc instability model with irradiation and disc evaporation/condensation.

  12. Brain-Machine Interface to Control a Prosthetic Arm with Monkey ECoGs during Periodic Movements

    Directory of Open Access Journals (Sweden)

    Soichiro eMorishita

    2014-12-01

    Full Text Available Brain Machine Interfaces (BMIs are promising technologies to rehabilitate the function of upper limbs in severely paralyzed patients. We succeeded in developing a BMI prosthetic arm for a monkey implanted with electrocorticogram (ECoG electrodes and trained in a reaching task. It had stability in preventing the misclassification of ECoG patterns. However, the latency was about 200 ms as a trade-off for the stability. To improve the response of this BMI prosthetic arm, the generation of a trigger event by decoding muscle activity was adopted. It was performed to predict integrated electromyograms (iEMGs from the ECoGs. Experiments were conducted to verify the availability of this method, and the results confirmed that the proposed method was superior to the conventional one. In addition, a performance test of the proposed method with actually achieved iEMGs instead of predicted iEMGs was performed, and we found that the motor intention is finely expressed through estimated muscle activity from brain activity rather than actual muscle activity.

  13. NuSTAR Observations of the Black Hole GS 1354-645: Evidence of Rapid Black Hole Spin

    CERN Document Server

    El-Batal, A M; Reynolds, M T; Boggs, S E; Chistensen, F E; Craig, W W; Fuerst, F; Hailey, C J; Harrison, F A; Stern, D K; Tomsick, J; Walton, D J; Zhang, W W

    2016-01-01

    We present the results of a NuSTAR study of the dynamically confirmed stellar-mass black hole GS 1354-645. The source was observed during its 2015 "hard" state outburst; we concentrate on spectra from two relatively bright phases. In the higher-flux observation, the broadband NuSTAR spectra reveal a clear, strong disk reflection spectrum, blurred by a degree that requires a black hole spin of a = cJ/GM^2 > 0.98 (1 sigma statistical limits only). The fits also require a high inclination: theta = 75(2) degrees. Strong "dips" are sometimes observed in the X-ray light curves of sources viewed at such an angle; these are absent, perhaps indicating that dips correspond to flared disk structures that only manifest at higher accretion rates. In the lower-flux observation, there is evidence of radial truncation of the thin accretion disk. We discuss these results in the context of spin in stellar-mass black holes, and inner accretion flow geometries at moderate accretion rates.

  14. An efficient procedure for plant organellar genome assembly, based on whole genome data from the 454 GS FLX sequencing platform

    Directory of Open Access Journals (Sweden)

    Zhang Tongwu

    2011-11-01

    Full Text Available Abstract Motivation Complete organellar genome sequences (chloroplasts and mitochondria provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology advances, it becomes the norm that a shotgun approach is used to obtain complete genome sequences. Therefore, to assemble organellar sequences from the whole genome, shotgun reads are inevitable. However, associated techniques are often cumbersome, time-consuming, and difficult, because true organellar DNA is difficult to separate efficiently from nuclear copies, which have been transferred to the nucleus through the course of evolution. Results We report a new, rapid procedure for plant chloroplast and mitochondrial genome sequencing and assembly using the Roche/454 GS FLX platform. Plant cells can contain multiple copies of the organellar genomes, and there is a significant correlation between the depth of sequence reads in contigs and the number of copies of the genome. Without isolating organellar DNA from the mixture of nuclear and organellar DNA for sequencing, we retrospectively extracted assembled contigs of either chloroplast or mitochondrial sequences from the whole genome shotgun data. Moreover, the contig connection graph property of Newbler (a platform-specific sequence assembler ensures an efficient final assembly. Using this procedure, we assembled both chloroplast and mitochondrial genomes of a resurrection plant, Boea hygrometrica, with high fidelity. We also present information and a minimal sequence dataset as a reference for the assembly of other plant organellar genomes.

  15. Evaluation of root water uptake in the ISBA-A-gs land surface model using agricultural yield statistics over France

    Science.gov (United States)

    Canal, N.; Calvet, J.-C.; Decharme, B.; Carrer, D.; Lafont, S.; Pigeon, G.

    2014-12-01

    The simulation of root water uptake in land surface models is affected by large uncertainties. The difficulty in mapping soil depth and in describing the capacity of plants to develop a rooting system is a major obstacle to the simulation of the terrestrial water cycle and to the representation of the impacts of drought. In this study, long time series of agricultural statistics are used to evaluate and constrain root water uptake models. The inter-annual variability of cereal grain yield and permanent grassland dry matter yield is simulated over France by the Interactions between Soil, Biosphere and Atmosphere, CO2-reactive (ISBA-A-gs) generic land surface model (LSM). The two soil profile schemes available in the model are used to simulate the above-ground biomass (Bag) of cereals and grasslands: a two-layer force-restore (FR-2L) bulk reservoir model and a multi-layer diffusion (DIF) model. The DIF model is implemented with or without deep soil layers below the root zone. The evaluation of the various root water uptake models is achieved by using the French agricultural statistics of Agreste over the 1994-2010 period at 45 cropland and 48 grassland départements, for a range of rooting depths. The number of départements where the simulated annual maximum Bag presents a significant correlation with the yield observations is used as a metric to benchmark the root water uptake models. Significant correlations (p value degrade) the representation of the inter-annual variability of the vegetation above-ground biomass. These results are particularly robust for grasslands, as calibrated simulations are able to represent the extreme 2003 and 2007 years corresponding to unfavourable and favourable fodder production, respectively.

  16. A 2.5 GS/s 14-bit D/A converter with 8 to 1 MUX

    Science.gov (United States)

    Jun'an, Zhang; Guangjun, Li; Ruitao, Zhang; Dongbing, Fu; Jiaoxue, Li; Yafeng, Wei; Bo, Yan; Jun, Liu; Ruzhang, Li

    2016-03-01

    A 2.5 GS/s 14-bit D/A converter (DAC) with 8 to 1 MUX is presented. This 14-bit DAC uses a “5+9” segment PMOS current-steering architecture. A bias circuit which ensures the PMOS current source obtains a larger output impedance under every PVT (process, source voltage and temperature) corner is also presented. The 8 to 1 MUX has a 3 stage structure, and a proper timing sequence is designed to ensure reliable data synthesis. A DEM function which is merged with a “5-31” decoder is used to improve the DAC's dynamic performance. This DAC is embedded in a 2.5 GHz direct digital frequency synthesizer (DDS) chip, and is implemented in a 0.18 μm CMOS technology, occupies 4.86 × 2. 28 mm2 including bond pads (DAC only), and the measured performance is SFDR > 40 dB (with and without DEM) for output signal frequency up to 1 GHz. Compared with other present published DACs with a non-analog-resample structure (means return-to-zero or quad-switch structure is unutilized), this paper DAC's clock frequency (2.5 GHz) and higher output frequency SFDR (> 40 dB, up to 1 GHz) has some competition. Project supported by the National Natural Science Foundation of China (Nos. 61006027, 61176030), the Research Foundation of Key Laboratory of Analog Integrated Circuit (Nos. 9140C0902120C09034, 9140c090204130c09042), and the Fundamental Research Funds for the Central Universities of China (No. ZYGX2012J003).

  17. Rossi X-Ray Timing Explorer Observations of an Outburst of Recurrent X-Ray Nova GS 1354-644

    International Nuclear Information System (INIS)

    We present the results of Rossi X-Ray Timing Explorer (RXTE) observations of GS 1354-644 during a modest outburst in 1997-1998. The source is one of a handful of black hole X-ray transients that are confirmed to be recurrent in X-rays. A 1987 outburst of the same source observed by Ginga was much brighter and showed a high/soft spectral state. In contrast, the 1997-1998 outburst showed a low/hard spectral state. Both states are typical of black hole binaries. The RXTE All-Sky Monitor observed an outburst duration of 150-200 days. PCA and HEXTE observations covered ∼70 days near the maximum of the light curve and during the flux decline. Throughout the observations, the spectrum can be approximated by Compton upscattering of soft photons by energetic electrons. The hot electron cloud has a temperature kT∼30 keV and optical depth τ∼4-5. To fit the data well, an additional iron fluorescent line and reflection component are required, which indicates the presence of optically thick cool material, most probably in the outer part of the accretion disk. Dramatic fast variability was observed and has been analyzed in the context of a shot noise model. The spectrum appeared to be softest at the peaks of the shot noise variability. The shape of the power spectrum was typical for black hole systems in a low/hard state. We note a qualitative difference in the shape of the dependence of fractional variability on energy when we compare systems with black holes and with neutron stars. Since it is difficult to discriminate these systems on spectral grounds, at least in their low/hard states, this new difference might be important. (c) 2000 The American Astronomical Society

  18. Plutonium(V/VI) Reduction by the Metal-Reducing Bacteria Geobacter metallireducens GS-15 and Shewanella oneidensis MR-1.

    Science.gov (United States)

    Icopini, Gary A; Lack, Joe G; Hersman, Larry E; Neu, Mary P; Boukhalfa, Hakim

    2009-06-01

    We examined the ability of the metal-reducing bacteria Geobacter metallireducens GS-15 and Shewanella oneidensis MR-1 to reduce Pu(VI) and Pu(V). Cell suspensions of both bacteria reduced oxidized Pu [a mixture of Pu(VI) and Pu(V)] to Pu(IV). The rate of plutonium reduction was similar to the rate of U(VI) reduction obtained under similar conditions for each bacteria. The rates of Pu(VI) and U(VI) reduction by cell suspensions of S. oneidensis were slightly higher than the rates observed with G. metallireducens. The reduced form of Pu was characterized as aggregates of nanoparticulates of Pu(IV). Transmission electron microscopy images of the solids obtained from the cultures after the reduction of Pu(VI) and Pu(V) by S. oneidensis show that the Pu precipitates have a crystalline structure. The nanoparticulates of Pu(IV) were precipitated on the surface of or within the cell walls of the bacteria. The production of Pu(III) was not observed, which indicates that Pu(IV) was the stable form of reduced Pu under these experimental conditions. Experiments examining the ability of these bacteria to use Pu(VI) as a terminal electron acceptor for growth were inconclusive. A slight increase in cell density was observed for both G. metallireducens and S. oneidensis when Pu(VI) was provided as the sole electron acceptor; however, Pu(VI) concentrations decreased similarly in both the experimental and control cultures. PMID:19363069

  19. CANGS: a user-friendly utility for processing and analyzing 454 GS-FLX data in biodiversity studies

    Directory of Open Access Journals (Sweden)

    Schlötterer Christian

    2010-01-01

    Full Text Available Abstract Background Next generation sequencing (NGS technologies have substantially increased the sequence output while the costs were dramatically reduced. In addition to the use in whole genome sequencing, the 454 GS-FLX platform is becoming a widely used tool for biodiversity surveys based on amplicon sequencing. In order to use NGS for biodiversity surveys, software tools are required, which perform quality control, trimming of the sequence reads, removal of PCR primers, and generation of input files for downstream analyses. A user-friendly software utility that carries out these steps is still lacking. Findings We developed CANGS (Cleaning and Analyzing Next Generation Sequences a flexible and user-friendly integrated software utility: CANGS is designed for amplicon based biodiversity surveys using the 454 sequencing platform. CANGS filters low quality sequences, removes PCR primers, filters singletons, identifies barcodes, and generates input files for downstream analyses. The downstream analyses rely either on third party software (e.g.: rarefaction analyses or CANGS-specific scripts. The latter include modules linking 454 sequences with the name of the closest taxonomic reference retrieved from the NCBI database and the sequence divergence between them. Our software can be easily adapted to handle sequencing projects with different amplicon sizes, primer sequences, and quality thresholds, which makes this software especially useful for non-bioinformaticians. Conclusion CANGS performs PCR primer clipping, filtering of low quality sequences, links sequences to NCBI taxonomy and provides input files for common rarefaction analysis software programs. CANGS is written in Perl and runs on Mac OS X/Linux and is available at http://i122server.vu-wien.ac.at/pop/software.html

  20. A cAMP Biosensor-Based High-Throughput Screening Assay for Identification of Gs-Coupled GPCR Ligands and Phosphodiesterase Inhibitors

    DEFF Research Database (Denmark)

    Vedel, Line; Bräuner-Osborne, Hans; Mathiesen, Jesper Mosolff

    2015-01-01

    Cyclic adenosine 3',5'-monophosphate (cAMP) is an important second messenger, and quantification of intracellular cAMP levels is essential in studies of G protein-coupled receptors (GPCRs). The intracellular cAMP levels are regulated by the adenylate cyclase (AC) upon activation of either Gs- or Gi......-coupled GPCRs, which leads to increased or decreased cAMP levels, respectively. Here we describe a real-time Förster resonance energy transfer (FRET)-based cAMP high-throughput screening (HTS) assay for identification and characterization of Gs-coupled GPCR ligands and phosphodiesterase (PDE) inhibitors...... resulted in prolonged signaling and enabled detection of weak partial agonists and/or ligands with low potency, which is highly advantageous in large HTS settings and hit identification. In addition, the assay enabled detection of β2AR inverse agonists and PDE inhibitors. High signal-to-noise ratios were...

  1. Discovery of triazolopyridinone GS-462808, a late sodium current inhibitor (Late INai) of the cardiac Nav1.5 channel with improved efficacy and potency relative to ranolazine.

    Science.gov (United States)

    Koltun, Dmitry O; Parkhill, Eric Q; Elzein, Elfatih; Kobayashi, Tetsuya; Jiang, Robert H; Li, Xiaofen; Perry, Thao D; Avila, Belem; Wang, Wei-Qun; Hirakawa, Ryoko; Smith-Maxwell, Catherine; Wu, Lin; Dhalla, Arvinder K; Rajamani, Sridharan; Mollova, Nevena; Stafford, Brian; Tang, Jennifer; Belardinelli, Luiz; Zablocki, Jeff A

    2016-07-01

    Previously we disclosed the discovery of potent Late INa current inhibitor 2 (GS-458967, IC50 of 333nM) that has a good separation of late versus peak Nav1.5 current, but did not have a favorable CNS safety window due to high brain penetration (3-fold higher partitioning into brain vs plasma) coupled with potent inhibition of brain sodium channel isoforms (Nav1.1, 1.2, 1.3). We increased the polar surface area from 50 to 84Å(2) by adding a carbonyl to the core and an oxadiazole ring resulting in 3 GS-462808 that had lower brain penetration and serendipitously lower activity at the brain isoforms. Compound 3 has an improved CNS window (>20 rat and dog) relative to 2, and improved anti-ischemic potency relative to ranolazine. The development of 3 was not pursued due to liver lesions in 7day rat toxicology studies. PMID:27038498

  2. Using the local gyrokinetic code, GS2, to investigate global ITG modes in tokamaks. (I) s-${\\alpha}$ model with profile and flow shear effects

    OpenAIRE

    Abdoul, P. A.; Dickinson, D.; Roach, C. M.; Wilson, H R

    2014-01-01

    This paper combines results from a local gyrokinetic code with analytical theory to reconstruct the global eigenmode structure of the linearly unstable ion-temperature-gradient (ITG) mode with adiabatic electrons. The simulations presented here employ the s-${\\alpha}$ tokamak equilibrium model. Local gyrokinetic calculations, using GS2 have been performed over a range of radial surfaces, x, and for ballooning phase angle, p, in the range -${\\pi} {\\leq} p {\\leq\\pi}$, to map out the complex loc...

  3. Epidemiology and Heritability of Major Depressive Disorder, Stratified by Age of Onset, Sex, and Illness Course in Generation Scotland: Scottish Family Health Study (GS:SFHS)

    OpenAIRE

    Ana Maria Fernandez-Pujals; Mark James Adams; Pippa Thomson; McKechanie, Andrew G.; Blackwood, Douglas H.R.; Smith, Blair H; Dominiczak, Anna F.; Morris, Andrew D.; Keith Matthews; Archie Campbell; Pamela Linksted; Haley, Chris S.; Deary, Ian J; Porteous, David J.; MacIntyre, Donald J.

    2015-01-01

    The heritability of Major Depressive Disorder (MDD) has been estimated at 37% based largely on twin studies that rely on contested assumptions. More recently, the heritability of MDD has been estimated on large populations from registries such as the Swedish, Finnish, and Chinese cohorts. Family-based designs utilise a number of different relationships and provide an alternative means of estimating heritability. Generation Scotland: Scottish Family Health Study (GS:SFHS) is a large (n = 20,19...

  4. Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System

    Science.gov (United States)

    Li, Meiru; Li, Xiaoxia; Zhou, Zejiao; Wu, Pingzhi; Fang, Maichun; Pan, Xiaoping; Lin, Qiupeng; Luo, Wanbin; Wu, Guojiang; Li, Hongqing

    2016-01-01

    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) systems have been successfully used as efficient tools for genome editing in a variety of species. We used the CRISPR/Cas9 system to mutate the Gn1a (Os01g0197700), DEP1 (Os09g0441900), GS3 (Os03g0407400), and IPA1 (Os08g0509600) genes of rice cultivar Zhonghua 11, genes which have been reported to function as regulators of grain number, panicle architecture, grain size and plant architecture, respectively. Analysis of the phenotypes and frequencies of edited genes in the first generation of transformed plants (T0) showed that the CRISPR/Cas9 system was highly efficient in inducing targeted gene editing, with the desired genes being edited in 42.5% (Gn1a), 67.5% (DEP1), 57.5% (GS3), and 27.5% (IPA1) of the transformed plants. The T2 generation of the gn1a, dep1, and gs3 mutants featured enhanced grain number, dense erect panicles, and larger grain size, respectively. Furthermore, semi-dwarf, and grain with long awn, phenotypes were observed in dep1 and gs3 mutants, respectively. The ipa1 mutants showed two contrasting phenotypes, having either fewer tillers or more tillers, depending on the changes induced in the OsmiR156 target region. In addition, we found that mutants with deletions occurred more frequently than previous reports had indicated and that off-targeting had taken place in highly similar target sequences. These results proved that multiple regulators of important traits can be modified in a single cultivar by CRISPR/Cas9, and thus facilitate the dissection of complex gene regulatory networks in the same genomic background and the stacking of important traits in cultivated varieties. PMID:27066031

  5. Modelling LAI, surface water and carbon fluxes at high-resolution over France: comparison of ISBA-A-gs and ORCHIDEE

    Directory of Open Access Journals (Sweden)

    S. Lafont

    2011-07-01

    Full Text Available The Leaf Area Index (LAI is a measure of the amount of photosynthetic leaves and governs the canopy conductance to water vapor and carbon dioxide. Four different estimates of LAI were compared over France: two LAI products derived from satellite remote sensing, and two LAI simulations derived from land surface modelling. The simulated LAI was produced by the ISBA-A-gs model and by the ORCHIDEE model (developed by CNRM-GAME and by IPSL, respectively, for the 1994–2007 period. The two models were driven by the same atmospheric variables and used the same land cover map (SAFRAN and ECOCLIMAP-II, respectively. The MODIS and CYCLOPES satellite LAI products were used. Both products were available from 2000 to 2007 and this relatively long period allowed to investigate the interannual and the seasonal variability of monthly LAI values. In particular the impact of the 2003 and 2005 droughts were analyzed. The two models presented contrasting results, with a difference of one month between the average leaf onset dates simulated by the two models, and a maximum interannual variability of LAI simulated at springtime by ORCHIDEE and at summertime by ISBA-A-gs. The comparison with the satellite LAI products showed that, in general, the seasonality was better represented by ORCHIDEE, while ISBA-A-gs tended to better represent the interannual variability, especially for grasslands. While the two models presented comparable values of net carbon fluxes, ORCHIDEE simulated much higher photosynthesis rates than ISBA-A-gs (+70 %, while providing lower transpiration estimates (−8 %.

  6. Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex

    OpenAIRE

    Liu, F; Weir, B S; Damm, U.; Crous, P.W.; Wang, Y; Liu, B.; Wang, M.(Central China Normal University, Wuhan, China); Zhang, M.; L. Cai

    2015-01-01

    We investigated the phylogenetic diversity of 144 Colletotrichum isolates associated with symptomatic and asymptomatic tissues of Camellia sinensis and other Camellia spp. from seven provinces in China (Fujian, Guizhou, Henan, Jiangxi, Sichuan, Yunnan, Zhejiang), and seven isolates obtained from other countries, including Indonesia, UK, and the USA. Based on multi-locus (ACT, ApMat, CAL, GAPDH, GS, ITS, TUB2) phylogenetic analyses and phenotypic characters, 11 species were distinguished, incl...

  7. Molecular analysis of the interaction between the intracellular loops of the human serotonin receptor type 6 (5-HT6) and the α subunit of GS protein

    International Nuclear Information System (INIS)

    The serotonin type 6 (5-HT6) receptor is a G-protein coupled receptor (GPCR) coupled to a stimulatory G-protein (GS). To identify the structural basis for the interaction of the 5-HT6 receptor with the GS protein, we have dissected the interaction between GST-fusion proteins containing the second intracellular loop (iL2), the third intracellular loop (iL3), or the C-terminal tail of the 5-HT6 receptor and the α subunit of GS (GαS). The direct interaction of iL3 and GαS was demonstrated by co-immunoprecipitation. Furthermore, the kinetic parameters of the interaction between iL3 and GαS were measured by surface plasmon resonance, and the apparent dissociation constant was determined to be 0.9 x 10-6 M. In contrast, the second intracellular loop and C-terminal tail regions showed negligible affinity to GαS. The critical residues within the iL3 region for the interaction with GαS were identified as conserved positively charged residues near the C-terminus of iL3 by measuring the cellular levels of cAMP produced in response to 5-HT stimulation of cells transfected with 5-HT6 receptor mutants

  8. 99mTc-HMPAO-Granulocyte Scintigraphy (GS), in the diagnosis and location of inflammation and infection: our experience in 307 cases

    International Nuclear Information System (INIS)

    Aim: to review our series of patients who underwent 99mTc-HMPAO Granulocyte Scintigraphy (GS) and evaluate the reliability of the technique and its accuracy in the diagnosis and location of inflammation and infection of bone and soft tissue. Materials and Methods: we have studied 307 patients, either to confirm or rule out the clinical suspicion of an inflammation/infection in bone (212 cases) or soft tissue (brain -16 cases-, thorax -10 cases-, abdomen -27 cases- and vascular -13 cases-) - or to investigate and locate the site of the disease in patients with undiagnosed fever (29 cases). Scans were acquired at 2-4 and 20-24 hours after the radiopharmaceutical injection. Radiochemical purity, labelling efficiency, sterility and granulocyte separation yield and vitality were routinely assayed. The final diagnosis was done on the ground of bacteriological and surgical data and clinical follow-up. Results: among the 212 patients studied owing to a suspected orthopaedic infection, GS was positive in 119 cases (56%), negative in 80 (38%) and undetermined in 13 (6%). Among the patients studied for soft tissue inflammation/infection, we found a positive GS in 6/16 (38%) cases in the group with suspected brain abscess, in 4/10 (40%) in that of a suspected thoracic disease, in 16/27 (59 %) among those studied for a suspected abdominal inflammation-infection and in 8/13 (62%) studies for a suspected vascular graft infection. Finally 15 of the 29 patients (52 %) studied for an undiagnosed fever had a positive GS. Overall sensitivity of GS was 92 % and specificity 84 %. Mean radiochemical purity of 99mTc-HMPAO was 95 % (90-99 %), mean granulocyte labelling efficiency 55.1% (41-85 %), granulocyte separation yield a mean purified fraction of 82 % (60-98%) and mean granulocyte vitality, assessed by Trypan blue test, was 98% (95-100 %); all sterility tests were negative. Conclusion: 99mTc-HMPAO Granulocyte Scintigraphy confirms its reliability as an accurate diagnostic procedure

  9. INTERACCIÓN DE LA GLUTAMINA SINTETASA (GS Y EL PÉPTIDO β -AMILOIDE COMO UNA ESTRATEGIA DE PURIFICACIÓN

    Directory of Open Access Journals (Sweden)

    Sonia Luz Albarracín

    2013-08-01

    Full Text Available La enfermedad de Alzheimer (EA esla forma de demencia más común en laedad adulta. Se manifiesta con la pérdidaprogresiva de la memoria a medida quelas neuronas en la corteza cerebral y elhipocampo mueren. En todas las formasde EA se evidencia aumento de la expresiónde diferentes proteínas, así comola presencia de agregados insolubles depéptido-β-amiloide (PBA. La glutaminasintetasa (GS es una enzima clave en elmetabolismo del glutamato y en la detoxificaciónde amonio (NH4+. Previamentese ha reportado una posible interacciónGS-PBA que puede estar asociada conEA. En este trabajo se realizó la purificaciónde la enzima cerebral de rata a partirde un extracto sometido a precipitaciónfraccionada con (NH42SO4 del 20-60 %de saturación y posteriormente a travésde cromatografías sucesivas de filtraciónen gel, intercambio iónico y afinidad. Elpeso molecular del complejo fue calculadoen 137 kDa por el orden de eluciónen la columna de filtración. Se identificóinteracción de la enzima con PBA1-40, lográndose la purificación de unasola banda de 45 kDa, correspondiente ala forma monomérica de la GS. En estetrabajo se presenta un nuevo método depurificación de la enzima y se demuestrala interacción de GS con el PBA. Se proponeque esta interacción GS-PBA puedeser uno de los procesos que se presentanen la enfermedad al explicar la reducciónde la actividad de la enzima en pacientes con EA, ya que podría alterar el cicloglutamato-glutamina y generar cambiosen el entorno celular que favorecen excitotoxicidadpor glutamato típica de losprocesos de neurodegeneración.

  10. Development of feed supplement Urea Molasses Multi nutrient Block (UMMB) using protein source from soy bean flour and gliricidia sepium (Gs) for ruminant animal

    International Nuclear Information System (INIS)

    The aim of this activities is to develop formula of feed supplement UMMB-Gs for ruminant animal. The development of feed supplement was carried out on a laboratory and field scale. The activities on laboratory scale include biological evaluation of feed supplement used isotope technique P-32 in order to measure ratio bacteria and protozoa and growth rate of microbial cell in rumen liquid by in vitro studies. Two feed supplement were developed, these were UMMB-TK and UMMB-Gs. Two UMMB-TK were produced at pesantren Al Hikmah and Famor Satwa. Gliricidia sepium meal combined with UMMB-BK were tested on Goat of PE, buffaloes and beef cattle by in vitro studies in order to measure growth rate of microbial cell in rumen liquid using P-32. On the next activity the effect of UMMB-Gs on production and fat concentration of milk from dairy cattle was done. Statistical analysis used were test, 3x3 latin square design and randomize block design respectively. Quality control of UMMB indicated that ratio of bacteria and protozoa was 14 : 1 on UMMB-BK formulas, whereas on UMMB-TK1 it was found 19 : 1 and UMMB-TK2 was 17 : 1 respectively. These results were better then control (grass only). The value of feed control was 1 : 4. The result of UMMB-BK combinated with Gs on laboratory scale was capable of increasing growth rate of microbial cell on rumen liquid of Goat PE, buffaloes and beef cattle. The values were 102.01%; 205.7% and 73.7% respectively compared to control. Field trial of UMMB-Gs increased milk production and fat concentration on dairy cattle. It mean that nuclear technique has a potential role on the finding of a new feed supplement formulas and capable of giving positive impact, when UMMB feed supplement was able to create job field for small business of UMMB product based on local feed resources. (author)

  11. Anti-EBOV GP IgGs Lacking α1-3-Galactose and Neu5Gc Prolong Survival and Decrease Blood Viral Load in EBOV-Infected Guinea Pigs

    Science.gov (United States)

    Reynard, Olivier; Jacquot, Frédéric; Evanno, Gwénaëlle; Mai, Hoa Le; Martinet, Bernard; Duvaux, Odile; Bach, Jean-Marie; Conchon, Sophie; Judor, Jean-Paul; Perota, Andrea; Lagutina, Irina; Duchi, Roberto; Lazzari, Giovanna; Le Berre, Ludmilla; Perreault, Hélène; Lheriteau, Elsa; Raoul, Hervé; Volchkov, Viktor; Galli, Cesare; Soulillou, Jean-Paul

    2016-01-01

    Polyclonal xenogenic IgGs, although having been used in the prevention and cure of severe infectious diseases, are highly immunogenic, which may restrict their usage in new applications such as Ebola hemorrhagic fever. IgG glycans display powerful xenogeneic antigens in humans, for example α1–3 Galactose and the glycolyl form of neuraminic acid Neu5Gc, and IgGs deprived of these key sugar epitopes may represent an advantage for passive immunotherapy. In this paper, we explored whether low immunogenicity IgGs had a protective effect on a guinea pig model of Ebola virus (EBOV) infection. For this purpose, a double knock-out pig lacking α1–3 Galactose and Neu5Gc was immunized against virus-like particles displaying surface EBOV glycoprotein GP. Following purification from serum, hyper-immune polyclonal IgGs were obtained, exhibiting an anti-EBOV GP titer of 1:100,000 and a virus neutralizing titer of 1:100. Guinea pigs were injected intramuscularly with purified IgGs on day 0 and day 3 post-EBOV infection. Compared to control animals treated with IgGs from non-immunized double KO pigs, the anti-EBOV IgGs-treated animals exhibited a significantly prolonged survival and a decreased virus load in blood on day 3. The data obtained indicated that IgGs lacking α1–3 Galactose and Neu5Gc, two highly immunogenic epitopes in humans, have a protective effect upon EBOV infection. PMID:27280712

  12. GsAPK, an ABA-activated and calcium-independent SnRK2-type kinase from G. soja, mediates the regulation of plant tolerance to salinity and ABA stress.

    Directory of Open Access Journals (Sweden)

    Liang Yang

    Full Text Available Plant Snf1 (sucrose non-fermenting-1 related protein kinase (SnRK, a subfamily of serine/threonine kinases, has been implicated as a crucial upstream regulator of ABA and osmotic signaling as in many other signaling cascades. In this paper, we have isolated a novel plant specific ABA activated calcium independent protein kinase (GsAPK from a highly salt tolerant plant, Glycine soja (50109, which is a member of the SnRK2 family. Subcellular localization studies using GFP fusion protein indicated that GsAPK is localized in the plasma membrane. We found that autophosphorylation and Myelin Basis Protein phosphorylation activity of GsAPK is only activated by ABA and the kinase activity also was observed when calcium was replaced by EGTA, suggesting its independence of calcium in enzyme activity. We also found that cold, salinity, drought, and ABA stress alter GsAPK gene transcripts and heterogonous overexpression of GsAPK in Arabidopsis alters plant tolerance to high salinity and ABA stress. In summary, we demonstrated that GsAPK is a Glycine soja ABA activated calcium independent SnRK-type kinase presumably involved in ABA mediated stress signal transduction.

  13. Assimilation of Leaf Area Index and Soil Wetness Index into the ISBA-A-gs land surface model over France

    Science.gov (United States)

    Barbu, A. L.; Calvet, J.-C.; Lafont, S.

    2012-04-01

    The development of a Land Data Assimilation System (LDAS) dedicated to carbon and water cycles is considered as a key aspect for monitoring activities of terrestrial carbon fluxes. It allows the assimilation of biophysical products in order to reduce the bias between the model simulations and the observations and have a positive impact on carbon and water fluxes. This work shows the benefits of data assimilation of Earth observations for the monitoring of vegetation status and carbon fluxes, in the framework of the GEOLAND2 project, co-funded by the European Commission within the GMES initiative in FP7. In this study, the SURFEX modelling platform developed at Meteo-France is used for describing the continental vegetation state, surface fluxes and soil moisture. It consists of the land surface model ISBA-A-gs that simulates photosynthesis and plant growth. The vegetation biomass and Leaf Area Index (LAI) evolve dynamically in response to weather and climate conditions. The ECOCLIMAP database provides detailed information about the land cover at a resolution of 1 km. Over the France domain, the most present ecosystem types are grasslands (32%), C3 crop lands (24%), deciduous forest (20%), bare soil (11%), and C4 crop lands (8%).The model also includes a representation of the soil moisture stress with two different types of drought responses for herbaceous vegetation and forests. A version of the Extended Kalman Filter (EKF) scheme is developed for the joint assimilation of satellite-derived surface soil moisture from ASCAT-25 km product, namely Soil Wetness Index (SWI-01) developed by TU-Wien, and remote sensing LAI product provided by GEOLAND2. The GEOLAND2 LAI product is derived from CYCLOPES V3.1 and MODIS collection 5 data. It is more consistent with an effective LAI for low LAI and close to the actual LAI for high values. The assimilation experiment was conducted across France at a spatial resolution of 8 km. The study period ranges from July 2007 to December

  14. STIS and GHRS Observations of Warm and Hot Gas Overlying the Scutum Supershell (GS 018-04+44)

    Science.gov (United States)

    Savage, Blair D.; Sembach, Kenneth R.; Howk, J. Christopher

    2001-02-01

    We present Space Telescope Imaging Spectrograph (STIS) and Goddard High Resolution Spectrograph (GHRS) observations of interstellar UV absorption toward HD 177989, a B0 III star at 4.9 kpc in the direction l=17.8d, b=-11.9d. The line of sight passes through the high-latitude ejecta of the Scutum supershell (GS 018-06+44), which is ~5° in diameter extending ~7° below the Galactic plane at a kinematic distance of ~3.5 kpc in the Scutum spiral arm. The observations with the STIS E140H and GHRS echelle B gratings provide far- and middle-UV spectra at resolutions (FWHM) of ~3 km s-1 and a signal-to-noise ratio (S/N) of ~20:1 to 50:1. The observations reveal strong and broad absorption in the lines of Si IV and C IV centered on LSR velocities of +18 and +42 km s-1 and weaker absorption from these ions near -50 and -13 km s-1. Weak absorption by N V extends over the full velocity range traced by Si IV and C IV. The +18 km s-1 high-ionization absorption likely occurs in gas ~400 pc below the Sagittarius spiral arm, while the extremely strong +42 km s-1 absorption occurs in highly ionized gas in the Scutum supershell at a distance of ~700 pc below the Galactic plane. The properties of the highly ionized gas associated with the Scutum supershell are similar to the gas found in radio loops I and IV; in both cases there is a strong enhancement in the column density of C IV without a corresponding increase in the column density of N V, which causes N(C IV)/N(N V) to be among the largest measured in the interstellar medium. The low-ionization absorption lines of N I, S II, Si II, and Fe II produce narrow absorption features at +37, +40 km s-1 and +55, +60 km s-1. The strength and kinematic properties of these absorption features bear no resemblance to those expected for the high-latitude neutral cloud seen in the H I 21 cm line. This may be due to the relatively low angular resolution (FWHM ~21') of the 21 cm observations. The kinematic relationships among the high

  15. 未来之争——奥迪A6 hybrid VS 雷克萨斯GS 450h

    Institute of Scientific and Technical Information of China (English)

    吴中元; 赵勇; 丛军(摄影)

    2013-01-01

    在未来的十年中,混合动力车型将逐渐得到普及,而有着技术优势的雷克萨斯已向各个细分市场引入其混动车型。实力同样雄厚的奥迪也不甘示弱,利用奥迪A6hybrid较高性价比的优势正向雷克萨斯GS450h发起挑战,拼争的不是现阶段的销量,而是未来。

  16. Study on characteristic differences of wheat 1Ax1 and 1Ax2* NILS obtained by transgenic HMW-GS 1Dx5 + 1Dy10 gene

    International Nuclear Information System (INIS)

    Use wheat 1Ax1 and 1Ax2* NILS obtained by transgenic HMW-GS 1Dx5 + 1Dy10 gene, characteristics differences have been studied. The two-year results showed that the statistical differences in seed quality parameters, farinogram parameters, extensigram parameters, botany characters and main agricultural characters between 1Ax1 and 1Ax2* were not significant. We considered that 08K860 and 08K871 are similar in hereditary background, 1Ax1 and 1Ax2* have identical contribute on quality. Breeder should attach to 1Ax1 and 1Ax2* identically on wheat breeding. (authors)

  17. Utjecaj parametara kaljenja čeličnog lijeva GS30Mn5 na svojstva utora užnica broda jaružara

    OpenAIRE

    MARUŠIĆ, Vlatko; Samardžić, Ivan; TODORIĆ, Petar; Rozing, Goran

    2012-01-01

    Jaružari su specijalna morska plovila kojima se materijal s morskog dna uklanja u cilju: oblikovanja - produbljivanja lučnih pristaništa i plovnih puteva, polaganja cjevovoda, održavanja pješčanih plaža i obale od erozije, exploatacije naftnih izvora, formiranja umjetnih otoka nasipavanjem pijeska… Na ispitnim uzorcima izrađenim od istog materijala kao i užnice (GS30Mn5) izvršene su probe navarivanja (REL postupkom). Nakon toga izvršeno je indukcijsko kaljenje, različitim parametrima. Kontro...

  18. Nucleon-pion-state contribution in lattice calculations of the nucleon charges $g_A,g_T$ and $g_S$

    CERN Document Server

    Bar, Oliver

    2016-01-01

    We employ leading order covariant chiral perturbation theory to compute the nucleon-pion-state contribution to the 3-point correlation functions one typically measures in lattice QCD to extract the isovector nucleon charges $g_A,g_T$ and $g_S$. We estimate the impact of the nucleon-pion-state contribution on both the plateau and the summation method for lattice simulations with physical pion masses. The nucleon-pion-state contribution results in an overestimation of all charges with both methods. The overestimation is roughly equal for the axial and the tensor charge, and about fifty percent larger for the scalar charge.

  19. Genome Sequencing of Giardia lamblia Genotypes A2 and B Isolates (DH and GS) and Comparative Analysis with the Genomes of Genotypes A1 and E (WB and Pig)

    Science.gov (United States)

    Adam, Rodney D.; Dahlstrom, Eric W.; Martens, Craig A.; Bruno, Daniel P.; Barbian, Kent D.; Ricklefs, Stacy M.; Hernandez, Matthew M.; Narla, Nirmala P.; Patel, Rima B.; Porcella, Stephen F.; Nash, Theodore E.

    2013-01-01

    Giardia lamblia (syn G. intestinalis, G. duodenalis) is the most common pathogenic intestinal parasite of humans worldwide and is a frequent cause of endemic and epidemic diarrhea. G. lamblia is divided into eight genotypes (A–H) which infect a wide range of mammals and humans, but human infections are caused by Genotypes A and B. To unambiguously determine the relationship among genotypes, we sequenced GS and DH (Genotypes B and A2) to high depth coverage and compared the assemblies with the nearly completed WB genome and draft sequencing surveys of Genotypes E (P15; pig isolate) and B (GS; human isolate). Our results identified DH as the smallest Giardia genome sequenced to date, while GS is the largest. Our open reading frame analyses and phylogenetic analyses showed that GS was more distant from the other three genomes than any of the other three were from each other. Whole-genome comparisons of DH_A2 and GS_B with the optically mapped WB_A1 demonstrated substantial synteny across all five chromosomes but also included a number of rearrangements, inversions, and chromosomal translocations that were more common toward the chromosome ends. However, the WB_A1/GS_B alignment demonstrated only about 70% sequence identity across the syntenic regions. Our findings add to information presented in previous reports suggesting that GS is a different species of Giardia as supported by the degree of genomic diversity, coding capacity, heterozygosity, phylogenetic distance, and known biological differences from WB_A1 and other G. lamblia genotypes. PMID:24307482

  20. Ultrasensitive electrochemical aptasensor for the detection of thrombin based on dual signal amplification strategy of Au@GS and DNA-CoPd NPs conjugates.

    Science.gov (United States)

    Wang, Yaoguang; Zhang, Yong; Yan, Tao; Fan, Dawei; Du, Bin; Ma, Hongmin; Wei, Qin

    2016-06-15

    In this work, an ultrasensitive electrochemical aptasensor for the detection of thrombin was developed based on Au nanoparticles decorated graphene sheet (Au@GS) and CoPd binary nanoparticles (CoPd NPs). A sulfydryl-labeled thrombin capture probe (Apt1) and a biotin-labeled thrombin reporter probe (Apt2) were designed to achieve a sandwich-type strategy. Au@GS was used as a sensing platform for the facile immobilization of Apt1 through Au-S bond, forming a sensing interface for thrombin. The specific recognition of thrombin induced the attachment of Apt2-CoPd NPs to the electrode. The labeled CoPd NPs showed good catalytic properties toward the reduction of H2O2, resulting in an amperometric signal. The amperometric response was correlated to the thrombin concentration in sample solutions. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful fabrication of the aptasensor. A linear response to thrombin in the range of 0.01-2.00ngmL(-1) with a low detection limit (5pgmL(-1)) was achieved. The proposed aptasensor showed good selectivity, good reproducibility and acceptable stability. This proposed strategy may find many potential applications in the detection of other biomolecules. PMID:26908183

  1. A sub-sampling 4-bit 1.056-GS/s flash ADC with a novel track and hold amplifierfor an IR-UWB receiver

    Institute of Scientific and Technical Information of China (English)

    Zhao Yi; Wang Shenjie; Qin Yajie; Hong Zhiliang

    2011-01-01

    A sub-sampling 4-bit 1.056-GS/s flash ADC with a novel track and hold amplifier (THA) in 0.13 μm CMOS for an impulse radio ultra-wideband (IR-UWB) receiver is presented.The challenge is in implementing a sub-sampling ADC with ultra-high input signal that further exceeds the Nyquist frequency.This paper presents,to our knowledge for the second time,a sub-sampling ADC with input signals above 4 GHz operating at a sampling rate of 1.056 GHz.In this design,a novel THA is proposed to solve the degradation in amplitude and improve the linearity of signal with frequency increasing to giga Hz.A resistive averaging technique is carefully analyzed to relieve noise aliasing.A low-offset latch using a zero-static power dynamic offset cancellation technique is further optimized to realize the requirements of speed,power consumption and noise aliasing.The measurement results reveal that the spurious free dynamic range of the ADC is 30.1 dB even if the input signal is 4.2 GHz sampled at 1.056 GS/s.The core power of the ADC is 30 mW,excluding all of the buffers,and the active area is 0.6 mm2.The ADC achieves a figure of merit of 3.75 p J/conversion-step.

  2. On Using SysML, DoDAF 2.0 and UPDM to Model the Architecture for the NOAA's Joint Polar Satellite System (JPSS) Ground System (GS)

    Science.gov (United States)

    Hayden, Jeffrey L.; Jeffries, Alan

    2012-01-01

    The JPSS Ground System is a lIexible system of systems responsible for telemetry, tracking & command (TT &C), data acquisition, routing and data processing services for a varied lIeet of satellites to support weather prediction, modeling and climate modeling. To assist in this engineering effort, architecture modeling tools are being employed to translate the former NPOESS baseline to the new JPSS baseline, The paper will focus on the methodology for the system engineering process and the use of these architecture modeling tools within that process, The Department of Defense Architecture Framework version 2,0 (DoDAF 2.0) viewpoints and views that are being used to describe the JPSS GS architecture are discussed. The Unified Profile for DoOAF and MODAF (UPDM) and Systems Modeling Language (SysML), as ' provided by extensions to the MagicDraw UML modeling tool, are used to develop the diagrams and tables that make up the architecture model. The model development process and structure are discussed, examples are shown, and details of handling the complexities of a large System of Systems (SoS), such as the JPSS GS, with an equally complex modeling tool, are described

  3. Sandwich-type electrochemical immunosensor for the detection of AFP based on Pd octahedral and APTES-M-CeO₂-GS as signal labels.

    Science.gov (United States)

    Wei, Yicheng; Li, Yan; Li, Na; Zhang, Yong; Yan, Tao; Ma, Hongmin; Wei, Qin

    2016-05-15

    In the present work, an ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy was designed for quantitative detection of alpha fetoprotein (AFP). Au nanoparticles with biocompatibility were electrodeposited on the surface of glassy carbon electrode (GCE) which can effectively capture and immobilize primary anti-AFP (Ab1) to significantly amplify the electrochemical signal. Graphene Oxide and CeO2 mesoporous nanocomposite functionalized by the 3-aminopropyltriethoxysilane supported Pd octahedral nanoparticles (Pd/APTES-M-CeO2-GS) were utilized as labels of detection anti-AFP (Ab2). Pd octahedral nanoparticles presented good catalytic activity towards the reduction of H2O2. Due to the large specific surface area and good adsorption properties of APTES-CeO2-GS nanocomposite, large amount of Pd octahedral nanoparticles could be immobilized, which could amplify the electrochemical signal and improve the sensitivity of the immunosensor. Under optimal conditions, the immunosensor exhibited wide linear range from 0.1 pg/mL to 50 ng/mL with a low detection limit of 0.033 pg/mL (S/N=3) for AFP detection. In addition, high sensitivity, excellent selectivity, good reproducibility and stability were obtained for the immunosensor, which has a promising application for quantitative detection of other tumor markers in clinical diagnosis. PMID:26745795

  4. Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex.

    Science.gov (United States)

    Liu, F; Weir, B S; Damm, U; Crous, P W; Wang, Y; Liu, B; Wang, M; Zhang, M; Cai, L

    2015-12-01

    We investigated the phylogenetic diversity of 144 Colletotrichum isolates associated with symptomatic and asymptomatic tissues of Camellia sinensis and other Camellia spp. from seven provinces in China (Fujian, Guizhou, Henan, Jiangxi, Sichuan, Yunnan, Zhejiang), and seven isolates obtained from other countries, including Indonesia, UK, and the USA. Based on multi-locus (ACT, ApMat, CAL, GAPDH, GS, ITS, TUB2) phylogenetic analyses and phenotypic characters, 11 species were distinguished, including nine well-characterised species (C. alienum, C. boninense, C. camelliae, C. cliviae, C. fioriniae, C. fructicola, C. gloeosporioides, C. karstii, C. sia-mense), and two novel species (C. henanense and C. jiangxiense). Of these, C. camelliae proved to be the most dominant and probably host specific taxon occurring on Camellia. An epitype is also designated for the latter species in this study. Colletotrichum jiangxiense is shown to be phylogenetically closely related to the coffee berry pathogen C. kahawae subsp. kahawae. Pathogenicity tests and the pairwise homoplasy index test suggest that C. jiangxiense and C. kahawae subsp. kahawae are two independent species. This study represents the first report of C. alienum and C. cliviae occurring on Camellia sinensis. In addition, our study demonstrated that the combined use of the loci ApMat and GS in a phylogenetic analysis is able to resolve all currently accepted species in the C. gloeosporioides species complex. PMID:26823629

  5. Comparing the accuracy of ES-BC, EIS-GS, and ES Oxi on body composition, autonomic nervous system activity, and cardiac output to standardized assessments

    Directory of Open Access Journals (Sweden)

    Lewis JE

    2011-09-01

    Full Text Available John E Lewis1, Stacey L Tannenbaum1, Jinrun Gao3, Angelica B Melillo1, Evan G Long1, Yaima Alonso2, Janet Konefal1, Judi M Woolger2, Susanna Leonard1, Prabjot K Singh1, Lawrence Chen1, Eduard Tiozzo1 1Department of Psychiatry and Behavioral Sciences, 2Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, 3State Farm Insurance, Bloomington, IL, USA Background and purpose: The Electro Sensor Complex (ESC is software that combines three devices using bioelectrical impedance, galvanic skin response, and spectrophotometry: (1 ES-BC (Electro Sensor-Body Composition; LD Technology, Miami, FL to assess body composition, (2 EIS-GS (Electro Interstitial Scan-Galvanic Skin; LD Technology to predict autonomic nervous system activity, and (3 ES Oxi (Electro Sensor Oxi; LD Technology to assess cardiac output. The objective of this study was to compare each to a standardized assessment: ES-BC to dual-energy X-ray absorptiometry (DXA, EIS-GS to heart rate variability, and ES Oxi to BioZ Dx Diagnostic System (BioZ Dx; SonoSite Inc, Bothell, WA. Patients and methods: The study was conducted in two waves. Fifty subjects were assessed for body composition and autonomic nervous system activity. Fifty-one subjects were assessed for cardiac output. Results: We found adequate relative and absolute agreement between ES-BC and DXA for fat mass (r = 0.97, P < 0.001 with ES-BC overestimating fat mass by 0.1 kg and for body fat percentage (r = 0.92, P < 0.001 with overestimation of fat percentage by 0.4%. For autonomic nervous system activity, we found marginal relative agreement between EIS-GS and heart rate variability by using EIS-GS as the predictor in a linear regression equation (adjusted R2 = 0.56, P = 0.03. For cardiac output, adequate relative and absolute agreement was found between ES Oxi and BioZ Dx at baseline (r = 0.60, P < 0.001, after the first exercise stage (r = 0.79, P < 0.001, and after the second exercise stage (r = 0.86, P

  6. Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes

    Directory of Open Access Journals (Sweden)

    Ma Meng

    2012-08-01

    Full Text Available Abstract Background The Barley stripe mosaic virus (BSMV-based vector has been developed and used for gene silencing in barley and wheat seedlings to assess gene functions in pathogen- or insect-resistance, but conditions for gene silencing in spikes and grains have not been evaluated. In this study, we explored the feasibility of using BSMV for gene silencing in wheat spikes or grains. Results Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after13 days post inoculation (dpi, and persisted until 30dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV:PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction of endogenous PDS transcript abundances, suggesting that BSMV:PDS was able to induce PDS silencing in wheat spikes and grains. Inoculation onto wheat spikes from heading to flowering stage was optimal for efficient silencing of PDS in wheat spikes. Furthermore, we used the BSMV-based system to reduce the transcript level of 1Bx14, a gene encoding for High-molecular-weight glutenin subunit 1Bx14 (HMW-GS 1Bx14, by 97 % in the grains of the BSMV:1Bx14 infected spikes at 15dpi, compared with that in BSMV:00 infected spikes, and the reduction persisted until at least 25 dpi. The amount of the HMW-GS 1Bx14 was also detectably decreased. The percentage of glutenin macropolymeric proteins in total proteins was significantly reduced in the grains of 1Bx14-silenced plants as compared with that in the grains of BSMV:00 infected control plants, indicating that HMW-GS 1Bx14 is one of major components participating in the formation of glutenin macropolymers in wheat grains. Conclusion This is one of the first reports of successful application of BSMV-based virus-induced-gene-silencing (VIGS for gene knockdown in wheat spikes and grains and its application in functional analysis of

  7. Epidemiology and Heritability of Major Depressive Disorder, Stratified by Age of Onset, Sex, and Illness Course in Generation Scotland: Scottish Family Health Study (GS:SFHS.

    Directory of Open Access Journals (Sweden)

    Ana Maria Fernandez-Pujals

    Full Text Available The heritability of Major Depressive Disorder (MDD has been estimated at 37% based largely on twin studies that rely on contested assumptions. More recently, the heritability of MDD has been estimated on large populations from registries such as the Swedish, Finnish, and Chinese cohorts. Family-based designs utilise a number of different relationships and provide an alternative means of estimating heritability. Generation Scotland: Scottish Family Health Study (GS:SFHS is a large (n = 20,198, family-based population study designed to identify the genetic determinants of common diseases, including Major Depressive Disorder. Two thousand seven hundred and six individuals were SCID diagnosed with MDD, 13.5% of the cohort, from which we inferred a population prevalence of 12.2% (95% credible interval: 11.4% to 13.1%. Increased risk of MDD was associated with being female, unemployed due to a disability, current smokers, former drinkers, and living in areas of greater social deprivation. The heritability of MDD in GS:SFHS was between 28% and 44%, estimated from a pedigree model. The genetic correlation of MDD between sexes, age of onset, and illness course were examined and showed strong genetic correlations. The genetic correlation between males and females with MDD was 0.75 (0.43 to 0.99; between earlier (≤ age 40 and later (> age 40 onset was 0.85 (0.66 to 0.98; and between single and recurrent episodic illness course was 0.87 (0.72 to 0.98. We found that the heritability of recurrent MDD illness course was significantly greater than the heritability of single MDD illness course. The study confirms a moderate genetic contribution to depression, with a small contribution of the common family environment (variance proportion = 0.07, CI: 0.01 to 0.15, and supports the relationship of MDD with previously identified risk factors. This study did not find robust support for genetic differences in MDD due to sex, age of onset, or illness course. However

  8. Biased Gs versus Gq proteins and β-arrestin signaling in the NK1 receptor determined by interactions in the water hydrogen bond network.

    Science.gov (United States)

    Valentin-Hansen, Louise; Frimurer, Thomas M; Mokrosinski, Jacek; Holliday, Nicholas D; Schwartz, Thue W

    2015-10-01

    X-ray structures, molecular dynamics simulations, and mutational analysis have previously indicated that an extended water hydrogen bond network between trans-membranes I-III, VI, and VII constitutes an allosteric interface essential for stabilizing different active and inactive helical constellations during the seven-trans-membrane receptor activation. The neurokinin-1 receptor signals efficiently through Gq, Gs, and β-arrestin when stimulated by substance P, but it lacks any sign of constitutive activity. In the water hydrogen bond network the neurokinin-1 has a unique Glu residue instead of the highly conserved AspII:10 (2.50). Here, we find that this GluII:10 occupies the space of a putative allosteric modulating Na(+) ion and makes direct inter-helical interactions in particular with SerIII:15 (3.39) and AsnVII:16 (7.49) of the NPXXY motif. Mutational changes in the interface between GluII:10 and AsnVII:16 created receptors that selectively signaled through the following: 1) Gq only; 2) β-arrestin only; and 3) Gq and β-arrestin but not through Gs. Interestingly, increased constitutive Gs but not Gq signaling was observed by Ala substitution of four out of the six core polar residues of the network, in particular SerIII:15. Three residues were essential for all three signaling pathways, i.e. the water-gating micro-switch residues TrpVI:13 (6.48) of the CWXP motif and TyrVII:20 (7.53) of the NPXXY motif plus the totally conserved AsnI:18 (1.50) stabilizing the kink in trans-membrane VII. It is concluded that the interface between position II:10 (2.50), III:15 (3.39), and VII:16 (7.49) in the center of the water hydrogen bond network constitutes a focal point for fine-tuning seven trans-membrane receptor conformations activating different signal transduction pathways. PMID:26269596

  9. Carbon steel protection in G.S. (Girlder sulfide) heavy water fabrication plants. Control of iron content at the final stage of passivation. Pt. 10

    International Nuclear Information System (INIS)

    This paper is part of a series which corresponds to the carbon steel behaviour as construction material for Girlder sulfide (G.S.) heavy water plants. The present work analyses the iron concentration study during passivation in the passivating fluid. At the beginning, during the formation of the most soluble sulfide -that is the mackinawite-, the iron concentration reaches more than 10 ppm. After some days, this iron concentration begins to decrease up to its stabilization under 0.1 ppm. This process, which occurs in the 9th. and 11th days, indicates that passivation is over, and that a pyrite and pyrrhotite-pyrite layer exists on the iron. Some differences exist between the results obtained and those previsible for the iron sulfides solubilities. In spite of these difficulties, the procedure is perfectly adequate to judge the passivation final stage. (Author)

  10. Development of utility for on-site recording of dose rate and program for data accumulation, mapping and management using GPS and GIS (DRaMM-GiGs)

    International Nuclear Information System (INIS)

    We developed the utility for on-site recording of dose rate and program for data accumulation, mapping and management using the Global Positioning System (GPS) and a geographic information system (GIS) (DRaMM-GiGs) to perform prompt and reliable investigations in a decontamination project. The system simultaneously accumulates data on dose rate, date, time and position, and integrates the accumulated data which are available to be viewed and analyzed on a map. The system incorporates a function which presets monitoring sites as a grid square, and allows to access easily to the preset monitoring sites using a personal digital assistant (PDA) with GPS even after removal of marks such as trees and bushes. It was confirmed that working time and manpower for monitoring and data analysis could be substantially reduced through preliminary operation. (author)

  11. Amelioration et validation du modele de fonctionnement de la végétation ISBA-A-gs: stress hydrique et flux de CO2

    OpenAIRE

    Rivalland, Vincent

    2003-01-01

    Les schémas de surface ont été développés dans le but de modéliser les échanges de chaleur et d'humidité entre les surfaces continentales et l'atmosphère. Parmi les éléments constitutifs de ces surfaces, la végétation joue un rôle important dans la dynamique des flux à l'interface. Mon travail de thèse a porté sur l'amélioration de la paramétrisation de la végétation dans le modèle de surface ISBA-A-gs (Calvet et al, 1998). Ce modèle est capable de résoudre les bilans énergétique et hydrique ...

  12. Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system.

    Science.gov (United States)

    Nakamura, Tsuyoshi; Omasa, Takeshi

    2015-09-01

    Therapeutic antibodies are commonly produced by high-expressing, clonal and recombinant Chinese hamster ovary (CHO) cell lines. Currently, CHO cells dominate as a commercial production host because of their ease of use, established regulatory track record, and safety profile. CHO-K1SV is a suspension, protein-free-adapted CHO-K1-derived cell line employing the glutamine synthetase (GS) gene expression system (GS-CHO expression system). The selection of high-producing mammalian cell lines is a crucial step in process development for the production of therapeutic antibodies. In general, cloning by the limiting dilution method is used to isolate high-producing monoclonal CHO cells. However, the limiting dilution method is time consuming and has a low probability of monoclonality. To minimize the duration and increase the probability of obtaining high-producing clones with high monoclonality, an automated single cell-based clone selector, the ClonePix FL system, is available. In this study, we applied the high-throughput ClonePix FL system for cell line development using CHO-K1SV cells and investigated efficient conditions for single cell-based clone selection. CHO-K1SV cell growth at the pre-picking stage was improved by optimizing the formulation of semi-solid medium. The efficiency of picking and cell growth at the post-picking stage was improved by optimization of the plating time without decreasing the diversity of clones. The conditions for selection, including the medium formulation, were the most important factors for the single cell-based clone selection system to construct a high-producing CHO cell line. PMID:25792187

  13. Spearfishing regulation benefits artisanal fisheries: the ReGS indicator and its application to a multiple-use Mediterranean marine protected area.

    Directory of Open Access Journals (Sweden)

    Delphine Rocklin

    Full Text Available The development of fishing efficiency coupled with an increase of fishing effort led to the overexploitation of numerous natural marine resources. In addition to this commercial pressure, the impact of recreational activities on fish assemblages remains barely known. Here we examined the impact of spearfishing limitation on resources in a marine protected area (MPA and the benefit it provides for the local artisanal fishery through the use of a novel indicator. We analysed trends in the fish assemblage composition using artisanal fisheries data collected in the Bonifacio Strait Natural Reserve (BSNR, a Mediterranean MPA where the spearfishing activity has been forbidden over 15% of its area. Fish species were pooled into three response groups according to their target level by spearfishing. We developed the new flexible ReGS indicator reflecting shifts in species assemblages according to the relative abundance of each response group facing external pressure. The catch per unit effort (CPUE increased by ca. 60% in the BSNR between 2000 and 2007, while the MPA was established in 1999. The gain of CPUE strongly depended on the considered response group: for the highly targeted group, the CPUE doubled while the CPUE of the untargeted group increased by only 15.5%. The ReGS value significantly increased from 0.31 to 0.45 (on a scale between 0 and 1 in the general perimeter of this MPA while it has reached a threshold of 0.43, considered as a reference point, in the area protected from spearfishing since 1982. Our results demonstrated that limiting recreational fishing by appropriate zoning in multiple-use MPAs represents a real benefit for artisanal fisheries. More generally we showed how our new indicator may reveal a wide range of impacts on coastal ecosystems such as global change or habitat degradation.

  14. 樱桃叶片气孔导度动态模拟研究%Study on Dynamic Simulation of Stomatal Conductance( Gs ) of Cherry(Prunus avium L.' Hongdeng' ) Leaf

    Institute of Scientific and Technical Information of China (English)

    高照全; 赵晨霞; 冯社章; 程建军

    2012-01-01

    [ Objective] The aim was to simulate the dynamic change law of cherry leaf s Gs under different weather by Gs model. [ Method] The experiment was conducted in a ' Hongdeng' cherry orchard. The parameters of the model were tested by the data observed in upper canopy from 2008 to 2011 during the growing seasons. [ Result] The diurnal course of Gs showed a double-peak curve on sunny days. And it reached the first peak of 240 mmol/(m2 · S) at mid-morning about 08:00 and the second at mid-afternoon about 16:00. The decline of Gs at noon was caused by lower RH and ψt. During a whole day (12 h) , average Gs was about 154. 36 mmol/( m2 · S) on sunny day. [ Conclusion] The study provides a scientific basis for using Gs model to simulate Gs of fruit tree leaf under different weather.%[目的]利用气孔导度(Gs)模型模拟红灯樱桃(Prunus avium L.‘Hongdeng’)叶片G,在不同气象条件下的动态变化规律.[方法]于2008~2011年在红灯樱桃园中,利用树冠上层叶片气体交换数据拟合相应参数.[结果]在晴天,樱桃上层叶片Gs呈双峰曲线,最大峰值出现在08:00左右,约240 mmol/( m2·s);第2峰值在16:00,气孔的“午休”现象主要与中午较低的RH和ψ1有关.晴天时,一天当中Gs的平均值为154.36 mmol/( m2·s).[结论]该研究为采用Gs模型来模拟不同气象条件下果树叶片G1提供了科学依据.

  15. GPR40 (FFAR1) – Combined Gs and Gq signaling in vitro is associated with robust incretin secretagogue action ex vivo and in vivo

    DEFF Research Database (Denmark)

    Pedersen, Maria Hauge; Bentsen, Marie Aare; Husted, Anna S;

    2015-01-01

    40 agonist TAK-875 decreased blood glucose but surprisingly without stimulating incretins. METHODS AND RESULTS: Here we find that GPR40 can signal through not only Gq and IP3 but also Gs and cAMP when stimulated with certain agonists such as AM-1638 and AM-5262 in contrast to the endogenous LCFA...... is concluded that stimulation of GPR40 by endogenous LCFAs or by Gq-only synthetic agonists result in a rather limited incretin response, whereas Gq + Gs GPR40 agonists stimulate incretin secretion robustly....

  16. The central engine of $\\gamma$-ray bursters

    CERN Document Server

    Kluzniak, W

    1998-01-01

    GRBs are thought to arise in relativistic blast-wave shocks at distances of 10 to 1000 AU from the point where the explosive energy is initially released. To account for the observed duration and variability of the gamma-ray emission in most GRBs, a central engine powering the shocks must remain active for several seconds to many minutes but must strongly fluctuate in its output on much shorter timescales. We show how a neutron star differentially rotating at millisecond periods (DROMP) could be such an engine. A magnetized DROMP would repeatedly wind up toroidal magnetic fields to about 10**17 G and only release the corresponding magnetic energy, when each buoyant magnetic field torus floats up to, and breaks through, the stellar surface. The resulting rapid sub-bursts, separated by relatively quiescent phases, repeat until the kinetic energy of differential rotation is exhausted by these events. Calculated values of the energy released and of the various timescales are in agreement with observations of GRBs...

  17. ICO amplicon NGS data analysis: a Web tool for variant detection in common high-risk hereditary cancer genes analyzed by amplicon GS Junior next-generation sequencing.

    Science.gov (United States)

    Lopez-Doriga, Adriana; Feliubadaló, Lídia; Menéndez, Mireia; Lopez-Doriga, Sergio; Morón-Duran, Francisco D; del Valle, Jesús; Tornero, Eva; Montes, Eva; Cuesta, Raquel; Campos, Olga; Gómez, Carolina; Pineda, Marta; González, Sara; Moreno, Victor; Capellá, Gabriel; Lázaro, Conxi

    2014-03-01

    Next-generation sequencing (NGS) has revolutionized genomic research and is set to have a major impact on genetic diagnostics thanks to the advent of benchtop sequencers and flexible kits for targeted libraries. Among the main hurdles in NGS are the difficulty of performing bioinformatic analysis of the huge volume of data generated and the high number of false positive calls that could be obtained, depending on the NGS technology and the analysis pipeline. Here, we present the development of a free and user-friendly Web data analysis tool that detects and filters sequence variants, provides coverage information, and allows the user to customize some basic parameters. The tool has been developed to provide accurate genetic analysis of targeted sequencing of common high-risk hereditary cancer genes using amplicon libraries run in a GS Junior System. The Web resource is linked to our own mutation database, to assist in the clinical classification of identified variants. We believe that this tool will greatly facilitate the use of the NGS approach in routine laboratories. PMID:24227591

  18. Inhibition of fibrous dysplasia via blocking Gsα with suramin sodium loaded with an alendronate-conjugated polymeric drug delivery system.

    Science.gov (United States)

    Lv, Mingming; Li, Xiao; Huang, Yu; Wang, Nan; Zhu, Xinyuan; Sun, Jian

    2016-07-21

    Suramin sodium (SS), which can directly inhibit the committed step of Gsα activation, seems to be a promising drug for treating fibrous dysplasia (FD). Therefore, how to efficiently deliver SS to the lesion site becomes an urgent problem to be solved. Here a bone-targeted and pH-sensitive drug delivery system was constructed to deliver SS for treating FD with high efficiency. The novel type of bone-targeted cationic hyperbranched poly(amine-ester) (HBPAE) was synthesized by the proton-transfer polymerization of triethanolamine and glycidyl methacrylate, followed by surface carboxyl-modification and then conjugation of an alendronate (ALE) bone-targeting moiety. The resultant Suc-HBPAE-ALE formed nanoparticles in aqueous solution, and SS could be encapsulated into the Suc-HBPAE-ALE nanoparticles via electrostatic attraction. The dynamic light scattering (DLS) and transmission electron microscopy (TEM) assays showed that the SS-loaded nanoparticles had a spherical morphology with a mean diameter of 65 nm. The strong affinity of Suc-HBPAE-ALE nanoparticles to bone was verified by the hydroxyapatite (HA) adsorbing experiment. The therapeutic potential of the SS-loaded Suc-HBPAE-ALE nanoparticles was evaluated via the methylthiazoletetrazolium (MTT) assay and flow cytometry (FCM) analysis against FD cells. The experimental results indicated that the SS-loaded Suc-HBPAE-ALE nanoparticles were a highly promising drug delivery system with high efficiency for inhibiting the proliferation of diseased FD cells. PMID:27180636

  19. Implementation of a 10.24 GS/s 12-bit Optoelectronics Analog-to- Digital Converter Based on a Polyphase Demultiplexing Architecture

    Directory of Open Access Journals (Sweden)

    C. Villa-Angulo

    2013-01-01

    Full Text Available In this paper we present the practical implementation of a high-speed polyphase sampling and demultiplexingarchitecture for optoelectronics analog-to-digital converters (OADCs. The architecture consists of a one-stage divideby-eight decimator circuit where optically-triggered samplers are cascaded to sample an analog input signal, anddemultiplex different phases of the sampled signal to yield low data rate for electronic quantization. Electrical-in toelectrical-out data format is maintained through the sampling, demultiplexing and quantization processes of thearchitecture thereby avoiding the need for electrical-to-optical and optical-to-electrical signal conversions. Weexperimentally demonstrate a 10.24 giga samples per second (GS/s, 12-bit resolution OADC system comprising theoptically-triggered sampling circuits integrated with commercial electronic quantizers. Measurements performed on theOADC yielded an effective bit resolution (ENOB of 10.3 bits, spurious free dynamic range (SFDR of -32 dB andsignal-to-noise and distortion ratio (SNDR of 63.7 dB.

  20. An optimally tuned ensemble of the "eb_go_gs" configuration of GENIE: parameter sensitivity and bifurcations in the Atlantic overturning circulation

    Directory of Open Access Journals (Sweden)

    R. Marsh

    2013-02-01

    Full Text Available The key physical parameters of the "eb_go_gs" configuration of GENIE, an Earth system Model of Intermediate Complexity (EMIC, are tuned using a multi-objective genetic algorithm. An ensemble of 90 parameter sets is tuned using two ocean and two atmospheric state variables as targets. These are "Pareto-optimal", representing a range of trade-offs between the four tuning targets. For the leading five parameter sets, simulations are further evaluated alongside a simulation with untuned "default" parameters, comparing selected variables and diagnostics that describe the state of the atmosphere, ocean and sea ice. One of these parameter sets is selected for further analysis of the objective function (error landscape in the vicinity of its tuned values. "Cliffs" along some dimensions motivate closer inspection of corresponding variations in the Atlantic meridional overturning circulation (AMOC. This reveals that bifurcations in the AMOC are highly sensitive to parameters that are not commonly associated with MOC stability. Specifically, the state of the AMOC is sensitive to parameters governing the wind-driven circulation and atmospheric heat transport. Five optimal parameter sets are recommended for future use of GENIE in the configuration presented here.

  1. Human anti-Aβ IgGs target conformational epitopes on synthetic dimer assemblies and the AD brain-derived peptide.

    Directory of Open Access Journals (Sweden)

    Alfred T Welzel

    Full Text Available Soluble non-fibrillar assemblies of amyloid-beta (Aβ and aggregated tau protein are the proximate synaptotoxic species associated with Alzheimer's disease (AD. Anti-Aβ immunotherapy is a promising and advanced therapeutic strategy, but the precise Aβ species to target is not yet known. Previously, we and others have shown that natural human IgGs (NAbs target diverse Aβ conformers and have therapeutic potential. We now demonstrate that these antibodies bound with nM avidity to conformational epitopes on plate-immobilized synthetic Aβ dimer assemblies, including synaptotoxic protofibrils, and targeted these conformers in solution. Importantly, NAbs also recognized Aβ extracted from the water-soluble phase of human AD brain, including species that migrated on denaturing PAGE as SDS-stable dimers. The critical reliance on Aβ's conformational state for NAb binding, and not a linear sequence epitope, was confirmed by the antibody's nM reactivity with plate-immobilized protofibrills, and weak uM binding to synthetic Aβ monomers and peptide fragments. The antibody's lack of reactivity against a linear sequence epitope was confirmed by our ability to isolate anti-Aβ NAbs from intravenous immunoglobulin using affinity matrices, immunoglobulin light chain fibrils and Cibacron blue, which had no sequence similarity with the peptide. These findings suggest that further investigations on the molecular basis and the therapeutic/diagnostic potential of anti-Aβ NAbs are warranted.

  2. Using the local gyrokinetic code, GS2, to investigate global ITG modes in tokamaks. (I) s-${\\alpha}$ model with profile and flow shear effects

    CERN Document Server

    Abdoul, P A; Roach, C M; Wilson, H R

    2014-01-01

    This paper combines results from a local gyrokinetic code with analytical theory to reconstruct the global eigenmode structure of the linearly unstable ion-temperature-gradient (ITG) mode with adiabatic electrons. The simulations presented here employ the s-${\\alpha}$ tokamak equilibrium model. Local gyrokinetic calculations, using GS2 have been performed over a range of radial surfaces, x, and for ballooning phase angle, p, in the range -${\\pi} {\\leq} p {\\leq\\pi}$, to map out the complex local mode frequency, ${\\Omega_0(x, p) = \\omega_0(x, p) + i\\gamma_0(x, p)}$. Assuming a quadratic radial profile for the drive, namely ${\\eta_i = L_n/L_T}$, (holding constant all other equilibrium profiles such as safety factor, magnetic shear etc.), ${\\Omega_0(x, p)}$ has a stationary point. The reconstructed global mode then sits on the outboard mid plane of the tokamak plasma, and is known as a conventional or isolated mode, with global growth rate, ${\\gamma}$ ~ Max[${\\gamma_0(x, p)}$], where ${\\gamma_0(x, p)}$ is the loc...

  3. Warm isostatic pressing (WIP'ing) of GS44 Si{sub 3}N{sub 4} FDC parts for defect removal

    Energy Technology Data Exchange (ETDEWEB)

    Wu Suxing; Rangarajan, Sriram; Dai Cheng; McCuiston, Ryan; Langrana, Noshir A.; Safari, Ahmad; Danforth, Stephen C.; Clancy, Richard B.; Whalen, Philip J

    2003-12-15

    Fused deposition of ceramics (FDC) is one of the developing solid freeform fabrication (SFF) techniques. The successful production of high performance ceramics by the FDC process requires that no defects exist in the green parts. However, build defects, such as missing roads, poorly bonded layers or sub-perimeter voids can be encountered in improperly built FDC parts. In this study, a method known as WIP'ing (warm isostatic pressing) was evaluated for its ability to eliminate existing defects in GS44 Si{sub 3}N{sub 4} green FDC parts. Analogous to CIP'ing (cold isostatic pressing), the green FDC parts were rubber bagged and loaded into a pressure chamber filled with water soluble oil at different temperatures, ranging from 30 to 90 deg. C, at pressures of up to 35 MPa. X-Ray radiography results indicated that at temperatures above 70 deg. C, WIP'ing was effective in closing the gaps of the intentionally placed void defects in FDC parts. However, WIP'ing above 70 deg. C was not effective in healing the defects completely. The fracture strengths of FDC parts with intentional added defects, WIP'ed above 70 deg. C were substantially lower than control samples.

  4. Use of agricultural statistics to verify the interannual variability in land surface models: a case study over France with ISBA-A-gs

    Directory of Open Access Journals (Sweden)

    J.-C. Calvet

    2012-01-01

    Full Text Available In order to verify the interannual variability of the above-ground biomass of herbaceous vegetation simulated by the ISBA-A-gs land surface model, within the SURFEX modelling platform, French agricultural statistics for C3 crops and grasslands were compared with the simulations for the 1994–2008 period. While excellent correlations are obtained for grasslands, representing the interannual variability of crops is more difficult. It is shown that, the Maximum Available soil Water Capacity (MaxAWC has a large influence on the correlation between the model and the agricultural statistics. In particular, high values of MaxAWC tend to reduce the impact of the climate interannual variability on the simulated biomass. Also, high values of MaxAWC allow the simulation of a negative trend in biomass production, in relation to a marked warming trend, of about 0.12 Kyr−1 on average, affecting the daily maximum air temperature during the growing period (April–June. This trend is particularly acute in Northern France. The estimates of MaxAWC for C3 crops and grasslands, currently used in SURFEX, are about 129 mm and do not vary much. Therefore, more accurate grid-cell values of this parameter are needed.

  5. Polarization transfer measurements for $^{12}{\\rm C}(\\vec{p},\\vec{n})^{12}{\\rm N (g.s.},1^+)$ at 296 MeV and nuclear correlation effects

    CERN Document Server

    Dozono, M; Ihara, E; Asaji, S; Fujita, K; Hatanake, K; Ichimura, M; Ishida, T; Kaneda, T; Matsubara, H; Nagasue, Y; Noro, T; Sakemi, Y; Shimizu, Y; Takeda, H; Tameshige, Y; Tamii, A; Yamada, Y

    2009-01-01

    Differential cross sections and complete sets of polarization observables are presented for the Gamow-Teller $^{12}{\\rm C}(\\vec{p},\\vec{n})^{12}{\\rm N}({\\rm g.s.},1^+)$ reaction at a bombarding energy of 296 MeV with momentum transfers $q$ of 0.1 to $2.2{\\rm fm}^{-1}$. The polarization transfer observables are used to deduce the spin-longitudinal cross section, $ID_q$, and spin-transverse cross sections, $ID_p$ and $ID_n$. The data are compared with calculations based on the distorted wave impulse approximation (DWIA) using shell-model wave functions. Significant differences between the experimental and theoretical results are observed for all three spin-dependent $ID_i$ at momentum transfers of $q \\gtrsim 0.5{\\rm fm}^{-1}$, suggesting the existence of nuclear correlations beyond the shell model. We also performed DWIA calculations employing random phase approximation (RPA) response functions and found that the observed discrepancy is partly resolved by the pionic and rho-mesonic correlation effects.

  6. Solvarmeanlægs energibesparelser

    DEFF Research Database (Denmark)

    Furbo, Simon; Fan, Jianhua

    /S, Sonnnenkraft Scandinavia A/S and Batec Solvarme A/S. Solar domestic hot water systems as well as solar combi systems are included in the investigations The houses have different auxiliary energy supply systems: Natural gas boilers, oil fired burners, electrical heating and district heating. Some of the houses......Energy savings for a number of new solar heating systems in one family houses have been determined by means of information on the energy consumption of the houses before and after installation of the solar heating systems. The investigated solar heating systems are marketed by Velux Danmark A...... have a second auxiliary energy supply system. The collector areas vary from 1.83 m² to 9.28 m². Some of the solar heating systems are based on energy units with a new integrated natural gas boiler and a heat storage for the solar heating system. The existing energy systems in the houses are for most of...

  7. Solvarmeanlægs energibesparelser

    OpenAIRE

    Furbo, Simon; Fan, Jianhua

    2011-01-01

    Energibesparelsen for en række nye solvarmeanlæg i enfamiliehuse er bestemt ved hjælp af oplysninger om husenes energiforbrug før og efter installationen af solvarmeanlæggene. Solvarmeanlæggene er fra Velux Danmark A/S, Sonnnenkraft Scandinavia A/S og Batec Solvarme A/S. Solvarmeanlæg til brugsvandsopvarmning og solvarmeanlæg til kombineret brugsvands- og rumopvarmning indgår i undersøgelserne. Husene har forskellige supplerende energianlæg: Naturgaskedel, oliefyr, elvarme og fjernvarme. Nogl...

  8. Caracterización del viroma de arn en tejido radical de Solanum phureja mediante pirosecuenciación 454 gs-flx

    Directory of Open Access Journals (Sweden)

    Pablo Gutiérrez-Sánchez

    2014-08-01

    Full Text Available Las enfermedades virales son una de las principales limitantes para la producción de papa en la región Andina al reducir los rendimientos de los cultivos, afectar la calidad de los tubérculos y limitar su utilidad como semilla. Los programas de manejo integrado de las enfermedades virales deben estar sustentados en el conocimiento previo de las especies de virus y sus variantes presentes en los cultivares establecidos en cada región y en la disponibilidad de herramientas de diagnóstico viral específicas y altamente sensibles. En esta investigación se caracterizó el viroma de ARN asociado a tejido radicular de una planta de Solanum phureja cultivar Criolla Colombia mediante la pirosecuenciación del ADNc utilizando el sistema 454 GS-FLX. Los resultados demostraron la coinfección de tres virus de ARN en las raíces analizadas: dos variantes de Potato virus S detectadas en el 49,7 % (PVS Col y 42,7 % (PVS A del total de reads asociados a cápsides virales, el Potato virus V (PVV (6,5% y el Potato leafroll virus (PLRV (1,1%. Adicionalmente, se identificaron al menos dos diferentes variantes genómicas para cada uno de los virus, lo que debe ser considerado para el diseño futuro de herramientas de detección molecular que apoyen los programas de certificación de tubérculo-semilla y mejoramiento genético de papa por resistencia a virus.

  9. Assimilation of Soil Wetness Index and Leaf Area Index into the ISBA-A-gs land surface model: grassland case study

    Directory of Open Access Journals (Sweden)

    A. L. Barbu

    2011-07-01

    Full Text Available The performance of the joint assimilation in a land surface model of a Soil Wetness Index (SWI product provided by an exponential filter together with Leaf Area Index (LAI is investigated. The data assimilation is evaluated with different setups using the SURFEX modeling platform, for a period of seven years (2001–2007, at the SMOSREX grassland site in southwestern France. The results obtained with a Simplified Extended Kalman Filter demonstrate the effectiveness of a joint data assimilation scheme when both SWI and Leaf Area Index are merged into the ISBA-A-gs land surface model. The assimilation of a retrieved Soil Wetness Index product presents several challenges that are investigated in this study. A significant improvement of around 13 % of the root-zone soil water content is obtained by assimilating dimensionless root-zone SWI data. For comparison, the assimilation of in situ surface soil moisture is considered as well. A lower impact on the root zone is noticed. Under specific conditions, the transfer of the information from the surface to the root zone was found not accurate. Also, our results indicate that the assimilation of in situ LAI data may correct a number of deficiencies in the model, such as low LAI values in the senescence phase by using a seasonal-dependent error definition for background and observations. In order to verify the specification of the errors for SWI and LAI products, a posteriori diagnostics are employed. This approach highlights the importance of the assimilation design on the quality of the analysis. The impact of data assimilation scheme on CO2 fluxes is also quantified by using measurements of net CO2 fluxes gathered at the SMOSREX site from 2005 to 2007. An improvement of about 5 % in terms of rms error is obtained.

  10. Assimilation of Soil Wetness Index and Leaf Area Index into the ISBA-A-gs land surface model: grassland case study

    Directory of Open Access Journals (Sweden)

    A. L. Barbu

    2011-02-01

    Full Text Available The performance of the joint assimilation in a land surface model of a Soil Wetness Index (SWI product provided by an exponential filter together with Leaf Area Index (LAI is investigated. The data assimilation is evaluated with different setups using the SURFEX modeling platform, for a period of seven years (2001–2007, at the SMOSREX grassland site in southwestern France. The results obtained with a Simplified Extended Kalman Filter demonstrate the effectiveness of a joint data assimilation scheme when both SWI and Leaf Area Index are merged into the ISBA-A-gs land surface model. The assimilation of a retrieved Soil Wetness Index product presents several challenges that are investigated in this study. A significant improvement of around 13% of the root-zone soil water content is obtained by assimilating dimensionless root-zone SWI data. For comparison, the assimilation of in situ surface soil moisture is considered as well. A lower impact on the root zone is noticed. Under specific conditions, the transfer of the information from the surface to the root zone was found not accurate. Also, our results indicate that the assimilation of in situ LAI data may correct a number of deficiencies in the model, such as low LAI values in the senescence phase by using a seasonal-dependent error definition for background and observations. In order to verify the specification of the errors for SWI and LAI products, a posteriori diagnostics are employed. This approach highlights the importance of the assimilation design on the quality of the analysis. The impact of data assimilation scheme on CO2 fluxes is also quantified by using measurements of net CO2 fluxes gathered at the SMOSREX site from 2005 to 2007. An improvement of about 5% in terms of rms error is obtained.

  11. Assimilation of Soil Wetness Index and Leaf Area Index into the ISBA-A-gs land surface model: grassland case study

    Science.gov (United States)

    Barbu, A. L.; Calvet, J.-C.; Mahfouf, J.-F.; Albergel, C.; Lafont, S.

    2011-07-01

    The performance of the joint assimilation in a land surface model of a Soil Wetness Index (SWI) product provided by an exponential filter together with Leaf Area Index (LAI) is investigated. The data assimilation is evaluated with different setups using the SURFEX modeling platform, for a period of seven years (2001-2007), at the SMOSREX grassland site in southwestern France. The results obtained with a Simplified Extended Kalman Filter demonstrate the effectiveness of a joint data assimilation scheme when both SWI and Leaf Area Index are merged into the ISBA-A-gs land surface model. The assimilation of a retrieved Soil Wetness Index product presents several challenges that are investigated in this study. A significant improvement of around 13 % of the root-zone soil water content is obtained by assimilating dimensionless root-zone SWI data. For comparison, the assimilation of in situ surface soil moisture is considered as well. A lower impact on the root zone is noticed. Under specific conditions, the transfer of the information from the surface to the root zone was found not accurate. Also, our results indicate that the assimilation of in situ LAI data may correct a number of deficiencies in the model, such as low LAI values in the senescence phase by using a seasonal-dependent error definition for background and observations. In order to verify the specification of the errors for SWI and LAI products, a posteriori diagnostics are employed. This approach highlights the importance of the assimilation design on the quality of the analysis. The impact of data assimilation scheme on CO2 fluxes is also quantified by using measurements of net CO2 fluxes gathered at the SMOSREX site from 2005 to 2007. An improvement of about 5 % in terms of rms error is obtained.

  12. An optimally tuned ensemble of the "eb_go_gs" configuration of GENIE: parameter sensitivity and bifurcations in the Atlantic overturning circulation

    Directory of Open Access Journals (Sweden)

    R. Marsh

    2013-10-01

    Full Text Available The key physical parameters for the "eb_go_gs" configuration of version 2.7.4 of GENIE, an Earth system model of intermediate complexity (EMIC, are tuned using a multi-objective genetic algorithm. An ensemble of 90 parameter sets is tuned using two ocean and two atmospheric state variables as targets. These are "Pareto-optimal", representing a range of trade-offs between the four tuning targets. For the leading five parameter sets, simulations are evaluated alongside a simulation with untuned "default" parameters, comparing selected variables and diagnostics that describe the state of the atmosphere, ocean and sea ice. Further experiments are undertaken with these selected parameter sets to compare equilibrium climate sensitivities and transient climate responses. The pattern of warming under doubled CO2 is strongly shaped by changes in the Atlantic meridional overturning circulation (AMOC, while the pattern and rate of warming under rising CO2 is closely linked to changing sea ice extent. One of the five tuned parameter sets is identified as marginally optimal, and the objective function (error landscape is further analysed in the vicinity of the tuned values of this parameter set. "Cliffs" along some dimensions motivate closer inspection of corresponding variations in the AMOC. This reveals that bifurcations in the AMOC are highly sensitive to parameters that are not typically associated with MOC stability. Specifically, the state of the AMOC is sensitive to parameters governing the wind-driven circulation and atmospheric heat transport. For the GENIE configuration presented here, the marginally optimal parameter set is recommended for single simulations, although the leading five parameter sets may be used in ensemble mode to admit a constrained degree of parametric uncertainty in climate prediction.

  13. 硬粒小麦品种Langdon低分子量谷蛋白亚基新基因的克隆%Cloning of Novel LMW-GS Genes from Durum Wheat Cultivar Langdon

    Institute of Scientific and Technical Information of China (English)

    高双成; 王世华; 史国安; 孔祥生; 李友军

    2013-01-01

    The low-molecular-weight glutenin subunits (LMW-GS) encoded at Glu-3 loci are important for the end-use quality of durum wheat.The present paper reported two novel LMW-GS genes (LMW-LDN1 and LMW-LDN2) isolated from durum wheat cultivar Langdon using PCR method.The full coding regions of LMW-LDN1 (GenBank accession number:DQ659333) and LMW-LDN2(GenBank accession number:DQ659334) were composed of 1005 and 894 nucleotides,which were translated into proteins of 334 and 297 amino acids,respectively.They all had the typical structure of LMW-GS genes.The two genes were assigned to Glu-A3 locus through the analysis of LDN1D(1A) and LDN1D(1B) substitution lines.%为了进一步了解小麦Glu-3位点的基因组成,采用PCR方法从硬粒小麦品种Langdon中克隆了2个低分子量谷蛋白亚基(LMW-GS)新基因,暂命名为LMW-LDN1、LMW-LDN2,GenBank注册号为DQ659333和DQ659334.这2个基因都具有LMW-GS的典型结构特征,编码区全长分别为1 005 bp和894 bp,编码334个和297个氨基酸.通过Langdon1D(1A)和Langdon1D(1B)代换系定位分析表明,LMW-LDN1和LMW-LDN2位于Glu-A3位点.

  14. Enhanced Heavy Metal Tolerance and Accumulation by Transgenic Sugar Beets Expressing Streptococcus thermophilus StGCS-GS in the Presence of Cd, Zn and Cu Alone or in Combination

    OpenAIRE

    Liu, Dali; An, Zhigang; Mao, Zijun; Ma, Longbiao; Lu, Zhenqiang

    2015-01-01

    Phytoremediation is a promising means of ameliorating heavy metal pollution through the use of transgenic plants as artificial hyperaccumulators. A novel Streptococcus thermophilus γ-glutamylcysteine synthetase-glutathione synthetase (StGCS-GS) that synthesizes glutathione (GSH) with limited feedback inhibition was overexpressed in sugar beet (Beta vulgaris L.), yielding three transgenic lines (s2, s4 and s5) with enhanced tolerance to different concentrations of cadmium, zinc and copper, as ...

  15. Antifungal Volatile Organic Compounds from the Endophyte Nodulisporium sp. Strain GS4d2II1a: a Qualitative Change in the Intraspecific and Interspecific Interactions with Pythium aphanidermatum.

    Science.gov (United States)

    Sánchez-Fernández, Rosa Elvira; Diaz, Daniel; Duarte, Georgina; Lappe-Oliveras, Patricia; Sánchez, Sergio; Macías-Rubalcava, Martha Lydia

    2016-02-01

    This study demonstrates volatile organic compounds (VOCs) production as one of the defense mechanisms of the antagonistic endophyte Nodulisporium sp. GS4d2II1a, and the volatile changes in two times of the fungal growth; and, as result of its intra and interspecific interactions with the plant pathogen Pythium aphanidermatum. The antifungal activity of the volatile and diffusible metabolites was evaluated by means of three types of antagonism bioassays and by organic extract agar dilution. VOCs were obtained by gas chromatography coupled to mass spectrometry from 3- and 5-day Nodulisporium sp. cultures, as well as from its interspecific in vitro antagonistic interaction with the oomycete P. aphanidermatum, and its intraspecific Nodulisporium sp.-Nodulisporium sp. interaction. The GS4d2II1a strain completely inhibited the growth of two fungi and seven oomycetes by replacing their mycelia in simple antagonism bioassays and by producing in vitro volatile and diffusible metabolites that acted synergistically in multiple antagonism bioassays. Additionally, VOCs inhibited the growth of three oomycetes and one fungus in antagonism bioassays using divided plates. A total of 70 VOCs were detected, mainly including mono and sesquiterpenes, especially eucalyptol and limonene. Multiple correspondence analysis revealed four different volatile profiles, showing that volatiles changed with the fungus age and its intra and interspecific interactions. The metabolites produced by Nodulisporium sp. GS4d2II1a could be useful for biological control of fungal and oomycetes plant pathogens of economically important crops. PMID:26408189

  16. Métodos de coloração de Roeser (1972: modificado - e Kropp (1972 visando a substituição do azul de astra por azul de alcião 8GS ou 8GX Staining methods of modified Roeser (1972 and Kropp (1972, aiming at substituing the astra blue by alcian blue 8GS or 8GX

    Directory of Open Access Journals (Sweden)

    Rebeca Luque

    1996-12-01

    Full Text Available Em folhas de Lychnophora ericoides, Coffea arabica e Nymphaea mexicana foram testados métodos de coloração baseados em Roeser (1972 modificado e Kropp (1972, visando a substituição do corante azul de astra por azul de alcião 8GS ou 8GX. As amostras foram fixadas em FAA, desidratadas em série butílica terciária e incluídas em parafina. Os cortes histológicos transversais foram corados segundo diferentes baterias de coloração, modificadas quanto ao tipo de corante usado, diferenciador e série de desidratação. As lâminas permanentes foram preparadas com bálsamo-do-canadá sintético. Os resultados obtidos evidenciaram que o método de Roeser (1972 modificado é melhor que o de Kropp (1972, nas condições deste experimento. O azul de astra pode ser substituído por azul de alcião 8GX e a desidratação pode ser em série isopropílica ou etílica, sem grande diferença entre elas. São discutidos os resultados provenientes das diferentes colorações.Staining methods based on modified Roeser (1972 as well as that of Kropp (1972 were done with leaves of Lychnophora ericoides, Coffea arabica and Nymphaea mexicana aiming at substituing the astra blue stain by alcian blue 8GS or 8GX. Samples were fixed in FAA, dehydrated in teit-butyl alcohol series and embedded in paraffin. The histological transversal sections were stained in different staining batteries, modified according to the type of stains and differentiators, and the series of dehydration. Permanent slides were prepared with synthetic Canada balsam. The results obtained indicate that the modified Roeser method (1972 is better than that of Kropp (1972 under present experimental conditions. The astra blue can be substituted by alcian blue 8GX and dehydration can be isopropylic or ethylic, with little difference between them. The results obtained with the different staining techniques used are discussed.

  17. Impact of climate, vegetation, soil and crop management variables on multi-year ISBA-A-gs simulations of evapotranspiration over a Mediterranean crop site

    Science.gov (United States)

    Garrigues, S.; Olioso, A.; Carrer, D.; Decharme, B.; Calvet, J.-C.; Martin, E.; Moulin, S.; Marloie, O.

    2015-10-01

    Generic land surface models are generally driven by large-scale data sets to describe the climate, the soil properties, the vegetation dynamic and the cropland management (irrigation). This paper investigates the uncertainties in these drivers and their impacts on the evapotranspiration (ET) simulated from the Interactions between Soil, Biosphere, and Atmosphere (ISBA-A-gs) land surface model over a 12-year Mediterranean crop succession. We evaluate the forcing data sets used in the standard implementation of ISBA over France where the model is driven by the SAFRAN (Système d'Analyse Fournissant des Renseignements Adaptés à la Nivologie) high spatial resolution atmospheric reanalysis, the leaf area index (LAI) time courses derived from the ECOCLIMAP-II land surface parameter database and the soil texture derived from the French soil database. For climate, we focus on the radiations and rainfall variables and we test additional data sets which include the ERA-Interim (ERA-I) low spatial resolution reanalysis, the Global Precipitation Climatology Centre data set (GPCC) and the MeteoSat Second Generation (MSG) satellite estimate of downwelling shortwave radiations. The evaluation of the drivers indicates very low bias in daily downwelling shortwave radiation for ERA-I (2.5 W m-2) compared to the negative biases found for SAFRAN (-10 W m-2) and the MSG satellite (-12 W m-2). Both SAFRAN and ERA-I underestimate downwelling longwave radiations by -12 and -16 W m-2, respectively. The SAFRAN and ERA-I/GPCC rainfall are slightly biased at daily and longer timescales (1 and 0.5 % of the mean rainfall measurement). The SAFRAN rainfall is more precise than the ERA-I/GPCC estimate which shows larger inter-annual variability in yearly rainfall error (up to 100 mm). The ECOCLIMAP-II LAI climatology does not properly resolve Mediterranean crop phenology and underestimates the bare soil period which leads to an overall overestimation of LAI over the crop succession. The

  18. Impact of climate, vegetation, soil and crop management variables on multi-year ISBA-A-gs simulations of evapotranspiration over a Mediterranean crop site

    Directory of Open Access Journals (Sweden)

    S. Garrigues

    2015-02-01

    Full Text Available Generic land surface models are generally driven by large-scale forcing datasets to describe the climate, the surface characteristics (soil texture, vegetation dynamic and the cropland management (irrigation. This paper investigates the errors in these forcing variables and their impacts on the evapotranspiration (ET simulated from the Interactions between Soil, Biosphere, and Atmosphere (ISBA-A-gs land surface model over a 12 year Mediterranean crop succession. We evaluate the forcing datasets used in the standard implementation of ISBA over France where the model is driven by the SAFRAN high spatial resolution atmospheric reanalysis, the Leaf Area Index (LAI cycles derived from the Ecoclimap-II land surface parameter database and the soil texture derived from the French soil database. For climate, we focus on the radiations and rainfall variables and we test additional datasets which includes the ERA-Interim low spatial resolution reanalysis, the Global Precipitation Climatology Centre dataset (GPCC and the MeteoSat Second Generation (MSG satellite estimate of downwelling shortwave radiations. The methodology consists in comparing the simulation achieved using large-scale forcing datasets with the simulation achieved using local observations for each forcing variable. The relative impacts of the forcing variables on simulated ET are compared with each other and with the model uncertainties triggered by errors in soil parameters. LAI and the lack of irrigation in the simulation generate the largest mean deviations in ET between the large-scale and the local-scale simulations (equivalent to 24 and 19 months of ET over 12 yr. The climate induces smaller mean deviations equivalent to 7–8 months of ET over 12 yr. The soil texture has the lowest impact (equivalent to 3 months of ET. However, the impact of errors in the forcing variables is smaller than the impact triggered by errors in the soil parameters (equivalent to 27 months of ET. The

  19. Generalized loss of particle and hole neutron spectroscopic intensity in 103 Ru: a sign of significant differences between the odd nucleus and the G.S. of both even neighbors

    International Nuclear Information System (INIS)

    New spectroscopic strength information extracted through the 102 Ru (d,p) 103 Ru reaction with the nuclear emulsion technique at the Sao Paulo Pelletron - Enge spectrograph, significantly extended the range and detail of previously available data. The particle strength detected, as has already been verified for the hole strength, is also considerably depleted with respect to the valence expectations, in contrast with what was obtained for 101 Ru. This fact is interpreted as a sign of significant differences between the states of 103 Ru and the G.S. of both even neighbors. (author)

  20. Generalized loss of particle and hole neutron spectroscopic intensity in {sup 103} Ru: a sign of significant differences between the odd nucleus and the G.S. of both even neighbors

    Energy Technology Data Exchange (ETDEWEB)

    Barbosa, M.D.L.; Duarte, J.M.; Borello-Lewin, T.; Ukita, G.M.; Gomes, L.C.; Horodynski-Matsushigue, L.B. [Sao Paulo Univ., SP (Brazil). Inst. de Fisica

    1997-12-31

    New spectroscopic strength information extracted through the {sup 102} Ru (d,p) {sup 103} Ru reaction with the nuclear emulsion technique at the Sao Paulo Pelletron - Enge spectrograph, significantly extended the range and detail of previously available data. The particle strength detected, as has already been verified for the hole strength, is also considerably depleted with respect to the valence expectations, in contrast with what was obtained for {sup 101} Ru. This fact is interpreted as a sign of significant differences between the states of {sup 103} Ru and the G.S. of both even neighbors. (author) 3 refs., 1 fig.; mbarbosa at if.usp.br

  1. Validez factorial de la adaptación al español del Maslach Burnout Inventory-General Survey The Factorial Validity of the Maslach Burnout Inventory-General Survey (MBI-GS) Spanish Version

    OpenAIRE

    Gil-Monte, Pedro R.

    2002-01-01

    Objetivo. Explorar la estructura factorial de una adaptación al castellano del "Maslach Burnout Inventory-General Survey" (MBI-GS). Material y métodos. La muestra la integraron 149 policías municipales de Tenerife, España, a quienes se les aplicó el cuestionario en 1999. Se realizó un análisis factorial mediante Componentes Principales y rotación Varimax. Resultados. Cuatro factores tuvieron eigenvalues mayores que 1. En el Factor I se agruparon los items de Eficacia Profesional, en el Factor...

  2. Identification of the Periodic Hard X-Ray Transient GRO J1849-03 with the X-Ray Pulsar GS 1843-02 = X1845-024 - a New Be/X-Ray Binary

    OpenAIRE

    Soffitta, P.; Tomsick, J. A.; Harmon, B.A.; Costa, E.; Ford, E. C.; M. Tavani(IASF of Rome/INAF); Zhang, S.N.; Kaaret, P.

    1997-01-01

    We identify the periodic transient hard X-ray source GRO J1849-03 with the transient x-ray pulsar GS 1843-02 = X1845-024 based on the detection of x-ray outbursts from X1845-024 coincident with hard x-ray outbursts of GRO J1849--03. Based on its spin period of 94.8 s and its orbital period of 241 days, we classify the system as a Be/X-ray binary.

  3. High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

    OpenAIRE

    Blanchard, Véronique; Gadkari, R.P; George, A.V.E.; Roy, R; Gerwig, G J; Leeflang, B.R.; Dighe, R R; Boelens, R.; Kamerling, J P

    2008-01-01

    The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to generate biologically active 15N-labeled glycohormones for conformational studies focused on the unravelling of the NMR structures in solution, the P. pastoris strains GS115 and X-33 were explored for the expression of human chorionic gonadotropin (phCG) and human follicle-stimulating hormone (phFSH). In agreement with recent investigations on the N-glycosylation of phCG, p...

  4. Carbon steel protection in G.S. (Girlder sulfide) plants. A protective layer formation in saturated solution of sulfohydric acid in water at pH = 4.43. Pt. 3

    International Nuclear Information System (INIS)

    As complement to the experiences on carbon steel passivation for its use in G.S. (Girlder sulfide) heavy water production plants, the results herein obtained are given, processing the material with a saturated solution at 2.3 MPa of full pressure and 125 temperature degree of H2S in NaOH 5 x 10-3 M, which, at an equilibrium point, the pH is 4.43. The characteristics, the composition and adherence to the layer formed and the corrosion velocity data are analyzed. (Author)

  5. 产蛋白酶菌株Bacillus subtilis GS-1发酵条件优化及蛋白酶水解应用初步研究%Optimization of Protease Activity from Bacillus subtilis GS-1 and Potential Applications in Protein Hydrolysis

    Institute of Scientific and Technical Information of China (English)

    王方华; 刘亮; 王永华; 杨博

    2012-01-01

    This study was conducted to optimize conditions for crude proteases activity from Bacillus sub tilis GS - 1 by Plackett-Burman ( PB) design and response surface methodology. Meanwhile, potential applications of proteases in protein hydrolysis were also determined. Three significant variables (NH4C1, initial pH value and cultural temperature) necessary for the enhancement of proteases activity were select ed by using PB design. Furthermore, an empirical model was developed to describe the relationships be tween these tested variables. Maximum enzymatic activity was attained at 372. 814 U/mL, with NH4C1 at 3. 768 g/L, pH at 5. 6 and temperature at 22. 4 ℃. Results of hydrolysis experiments indicated that the protease more prefer to hydrolysis proteins from animal source than that from plant source. The protease was prone to hydrolyze chemical bond that composed by amino acids of Glu, Val, He, Leu, Phe or Arg, and had great potential for applications in protein hydrolysis.%利用Plackett-Burman(PB)试验和相应面优化方法对枯草杆菌GS-1菌株发酵产蛋白酶的条件进行优化,以期获得高活性的蛋白酶发酵液.同时,将发酵所产蛋白酶用于蛋白水解实验,以进一步考察蛋白酶的水解特性.首先,通过PB试验,筛选出3个影响菌株发酵过程中蛋白酶活力的主要因子(氯化铵、初始pH值及培养温度).其次,通过响应面优化法,进一步探讨各参数之间的相互关系并最终拟合得出发酵产酶活的方程.结果表明,在氯化铵(3.768g/L),初始pH(5.6)以及培养温度22.4℃的条件下,发酵液中蛋白酶的最高酶活力能达到372.814 U/mL.水解试验结果表明:相对于植物来源的蛋白,该蛋白酶粗提物对于动物来源的蛋白具有较大的水解偏好型.该蛋白酶粗提物对于由谷氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸或精氨酸所组成的化学键有较强的水解偏好性.以上结果表明:该菌株发酵所产的蛋白酶在

  6. Decays of the Three Top Contributors to the Reactor ν[over ¯]_{e} High-Energy Spectrum, ^{92}Rb, ^{96gs}Y, and ^{142}Cs, Studied with Total Absorption Spectroscopy.

    Science.gov (United States)

    Rasco, B C; Wolińska-Cichocka, M; Fijałkowska, A; Rykaczewski, K P; Karny, M; Grzywacz, R K; Goetz, K C; Gross, C J; Stracener, D W; Zganjar, E F; Batchelder, J C; Blackmon, J C; Brewer, N T; Go, S; Heffron, B; King, T; Matta, J T; Miernik, K; Nesaraja, C D; Paulauskas, S V; Rajabali, M M; Wang, E H; Winger, J A; Xiao, Y; Zachary, C J

    2016-08-26

    We report total absorption spectroscopy measurements of ^{92}Rb, ^{96gs}Y, and ^{142}Cs β decays, which are the most important contributors to the high energy ν[over ¯]_{e} spectral shape in nuclear reactors. These three β decays contribute 43% of the ν[over ¯]_{e} flux near 5.5 MeV emitted by nuclear reactors. This ν[over ¯]_{e} energy is particularly interesting due to spectral features recently observed in several experiments including the Daya Bay, Double Chooz, and RENO Collaborations. Measurements were conducted at Oak Ridge National Laboratory by means of proton-induced fission of ^{238}U with on-line mass separation of fission fragments and the Modular Total Absorption Spectrometer. We observe a β-decay pattern that is similar to recent measurements of ^{92}Rb, with a ground-state to ground-state β feeding of 91(3)%. We verify the ^{96gs}Y ground-state to ground-state β feeding of 95.5(20)%. Our measurements substantially modify the β-decay feedings of ^{142}Cs, reducing the β feeding to ^{142}Ba states below 2 MeV by 32% when compared with the latest evaluations. Our results increase the discrepancy between the observed and the expected reactor ν[over ¯]_{e} flux between 5 and 7 MeV, the maximum excess increases from ∼10% to ∼12%. PMID:27610847

  7. X-ray variability with spectral state transitions in NS-LMXBs observed with MAXI/GSC and Swift/BAT

    Science.gov (United States)

    Asai, Kazumi; Mihara, Tatehiro; Matsuoka, Masaru; Sugizaki, Mutsumi

    2015-10-01

    X-ray variabilities with spectral state transitions in bright low-mass X-ray binaries containing a neutron star are investigated by using the one-day bin light curves of MAXI/GSC (Gas Slit Camera) and Swift/BAT (Burst Alert Telescope). Four sources (4U 1636-536, 4U 1705-44, 4U 1608-52, and GS 1826-238) exhibited small-amplitude X-ray variabilities with spectral state transitions. Such "mini-outbursts" were characterized by smaller amplitudes (several times) and shorter duration (less than several tens of days) than those of "normal outbursts." A theoretical model of disk instability by Mineshige and Osaki (PASJ, 37, 1, 1985) predicts both large-amplitude outbursts and small-amplitude variabilities. We interpret the normal outbursts as the former prediction of this model, and the mini-outbursts as the latter. Here, we can also call the mini-outburst a "purr-type outburst" referring to the theoretical work. We suggest that similar variabilities lasting for several tens of days without spectral state transitions, which are often observed in the hard state, may be repeats of mini-outbursts.

  8. Possible hard X-ray shortages in bursts from KS 1731-260 and 4U 1705-44

    CERN Document Server

    Ji, Long; Chen, YuPeng; Zhang, Shuang-Nan; Kretschmar, Peter; Wang, Jian-Min; Li, Jian

    2014-01-01

    Aims: A hard X-ray shortage, implying the cooling of the corona, was observed during bursts of IGR J17473-272, 4U 1636-536, Aql X-1, and GS 1826-238. Apart from these four sources, we investigate here an atoll sample, in which the number of bursts for each source is larger than 5, to explore the possible additional hard X-ray shortage during {\\it Rossi X-ray timing explorer (RXTE)} era. Methods: According to the source catalog that shows type-I bursts, we analyzed all the available pointing observations of these sources carried out by the {\\it RXTE} proportional counter array (PCA). We grouped and combined the bursts according to their outburst states and searched for the possible hard X-ray shortage while bursting. Results: We found that the island states of KS 1731-260 and 4U 1705-44 show a hard X-ray shortage at significant levels of 4.5 and 4.7 $\\sigma$ and a systematic time lag of $0.9 \\pm 2.1$ s and $2.5 \\pm 2.0$ s with respect to the soft X-rays, respectively. While in their banana branches and other s...

  9. K-SHELL PHOTOABSORPTION OF MAGNESIUM IONS

    Energy Technology Data Exchange (ETDEWEB)

    Hasoğlu, M. F. [Department of Computer Engineering, Hasan Kalyoncu University, 27100 Sahinbey, Gaziantep (Turkey); Abdel-Naby, Sh. A. [Department of Physics, Auburn University, Auburn, AL 36849 (United States); Gatuzz, E.; Mendoza, C. [Centro de Física, Instituto Venezolano de Investigaciones Científicas, Caracas 1020 (Venezuela, Bolivarian Republic of); García, J. [Harvard-Smithsonian Center for Astrophysics, MS-6, 60 Garden Street, Cambridge, MA 02138 (United States); Kallman, T. R. [NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Gorczyca, T. W. [Department of Physics, Western Michigan University, Kalamazoo, MI 49008-5252 (United States)

    2014-09-01

    X-ray photoabsorption cross sections have been computed for all magnesium ions with three or more electrons using the R-matrix method. A comparison with other available data for Mg II-Mg X shows good qualitative agreement in the resultant resonance shapes. However, for the lower ionization stages, and for singly ionized Mg II in particular, the previous R-matrix results overestimate the K-edge position due to the neglect of important orbital relaxation effects, and a global shift downward in photon energy of those cross sections is therefore warranted. We have found that the cross sections for Mg I and Mg II are further complicated by the M-shell (n = 3) occupancy. As a result, the treatment of spectator Auger decay of 1s → np resonances using a method based on multichannel quantum defect theory and an optical potential becomes problematic, making it necessary to implement an alternative, approximate treatment of Auger decay for neutral Mg I. The new cross sections are used to fit the Mg K edge in XMM-Newton spectra of the low-mass X-ray binary GS 1826-238, where most of the interstellar Mg is found to be in ionized form.

  10. X-ray variability with spectral state transitions in NS-LMXBs observed with MAXI/GSC and Swift/BAT

    CERN Document Server

    Asai, Kazumi; Matsuoka, Masaru; Sugizaki, Mutsumi

    2015-01-01

    X-ray variabilities with spectral state transitions in bright low mass X-ray binaries containing a neutron star are investigated by using the one-day bin light curves of MAXI/GSC (Gas Slit Camera) and Swift/BAT (Burst Alert Telescope). Four sources (4U 1636$-$536, 4U 1705$-$44, 4U 1608$-$52, and GS 1826$-$238) exhibited small-amplitude X-ray variabilities with spectral state transitions. Such "mini-outbursts" were characterized by smaller amplitudes (several times) and shorter duration (less than several tens of days) than those of "normal outbursts." Theoretical model of disk instability by Mineshige and Osaki (PASJ, 37, 1, 1985) predicts both large-amplitude outbursts and small-amplitude variabilities. We interpret the normal outbursts as the former prediction of this model, and the mini-outbursts as the latter. Here, we can also call the mini-outburst as "purr-type outburst" referring to theoretical work. We suggest that similar variabilities lasting for several tens of days without spectral state transiti...

  11. Gyromagnetic gs factors of the spin-1/2 particles in the (1/2+-1/2--3/2-) triad of the four-vector spinor, ψμ, irreducibility and linearity

    International Nuclear Information System (INIS)

    The gauged Klein–Gordon equation, extended by a gsσμνFμν/4 interaction, the contraction of the electromagnetic field strength tensor, Fμν, with the generators, σμν/2, of the Lorentz group in (1/2, 0) ⊕ (0, 1/2), and gs being the gyromagnetic factor, is examined with the aim to find out as to what extent it qualifies as a wave equation for general relativistic spin-1/2 particles transforming as (1/2, 0) ⊕ (0, 1/2) and possibly distinct from the Dirac fermions. This equation can be viewed as the generalization of the gs = 2 case, known under the name of the Feynman–Gell-Mann equation, the only one which allows for a bilinearization into the gauged Dirac equation and its conjugate. At the same time, it is well-known a fact that a gs = 2 value can also be obtained upon the bilinearization of the nonrelativistic Schroedinger into nonrelativistic Pauli equations. The inevitable conclusion is that it must not be necessarily relativity which fixes the gyromagnetic factor of the electron to g(1/2) = 2, but rather the specific form of the primordial quadratic wave equation obeyed by it, that is amenable to a linearization. The fact is that space-time symmetries alone define solely the kinematic properties of the particles and neither fix the values of their interacting constants, nor do they necessarily prescribe linear Lagrangians. Information on such properties has to be obtained from additional physical inputs involving the dynamics. We here provide an example in support of the latter statement. Our case is that the spin-1/2- fermion residing within the four-vector spinor triad, ψμ ~ (1/2+-1/2--3/2-), whose sectors at the free particle level are interconnected by spin-up and spin-down ladder operators, does not allow for a description within a linear framework at the interacting level. Upon gauging, despite transforming according to the irreducible (1/2, 1) ⊕ (1, 1/2) building block of ψμ, and being described by 16-dimensional four-vector spinors

  12. Gyromagnetic gs factors of the spin-1/2 particles in the (1/2+-1/2--3/2-) triad of the four-vector spinor, ψμ, irreducibility and linearity

    Science.gov (United States)

    Delgado Acosta, E. G.; Banda Guzmán, V. M.; Kirchbach, M.

    2015-07-01

    The gauged Klein-Gordon equation, extended by a gsσμνFμν/4 interaction, the contraction of the electromagnetic field strength tensor, Fμν, with the generators, σμν/2, of the Lorentz group in (1/2, 0) ⊕ (0, 1/2), and gs being the gyromagnetic factor, is examined with the aim to find out as to what extent it qualifies as a wave equation for general relativistic spin-1/2 particles transforming as (1/2, 0) ⊕ (0, 1/2) and possibly distinct from the Dirac fermions. This equation can be viewed as the generalization of the gs = 2 case, known under the name of the Feynman-Gell-Mann equation, the only one which allows for a bilinearization into the gauged Dirac equation and its conjugate. At the same time, it is well-known a fact that a gs = 2 value can also be obtained upon the bilinearization of the nonrelativistic Schrödinger into nonrelativistic Pauli equations. The inevitable conclusion is that it must not be necessarily relativity which fixes the gyromagnetic factor of the electron to g(1/2) = 2, but rather the specific form of the primordial quadratic wave equation obeyed by it, that is amenable to a linearization. The fact is that space-time symmetries alone define solely the kinematic properties of the particles and neither fix the values of their interacting constants, nor do they necessarily prescribe linear Lagrangians. Information on such properties has to be obtained from additional physical inputs involving the dynamics. We here provide an example in support of the latter statement. Our case is that the spin-1/2- fermion residing within the four-vector spinor triad, ψμ (1/2+-1/2--3/2-), whose sectors at the free particle level are interconnected by spin-up and spin-down ladder operators, does not allow for a description within a linear framework at the interacting level. Upon gauging, despite transforming according to the irreducible (1/2, 1) ⊕ (1, 1/2) building block of ψμ, and being described by 16-dimensional four-vector spinors, though

  13. Geotech Smart24 data acquisition system input terminated noise seismic response adjusted test : StreckeisenSTS2-low and high gain, Guralp CMG3T and Geotech GS13 seismometers.

    Energy Technology Data Exchange (ETDEWEB)

    Rembold, Randy Kai; Hart, Darren M.; Harris, James Mark

    2008-08-01

    Sandia National Laboratories has tested, evaluated and reported on the Geotech Smart24 data acquisition system with active Fortezza crypto card data signing and authentication in SAND2008-. One test, Input Terminated Noise, allows us to characterize the self-noise of the Smart24 system. By computing the power spectral density (PSD) of the input terminated noise time series data set and correcting for the instrument response of different seismometers, the resulting spectrum can be compared to the USGS new low noise model (NLNM) of Peterson (1996), and determine the ability of the matched system of seismometer and Smart24 to be quiet enough for any general deployment location. Four seismometer models were evaluated: the Streckeisen STS2-Low and High Gain, Guralp CMG3T and Geotech GS13 models. Each has a unique pass-band as defined by the frequency band of the instrument corrected noise spectrum that falls below the new low-noise model.

  14. [Smoļenskas-Rīgas aktis 13. gs.-14. gs. pirmā puse : kompleksa "Moscowitica-Ruthenica" dokumenti par Smoļenskas un Rīgas attiecībām = Смоленско-рижские акты XIII в.-первая половина XIV в. : документ�

    Index Scriptorium Estoniae

    Levans, Andris, 1968-

    2011-01-01

    Arvustus: Smoļenskas-Rīgas aktis 13. gs.-14. gs. pirmā puse : kompleksa "Moscowitica-Ruthenica" dokumenti par Smoļenskas un Rīgas attiecībām = Смоленско-рижские акты XIII в.-первая половина XIV в. : документы комплекса Moscowitica-Ruthenica об отношениях Смоленска и Риги /koostajad Leksanders Ivanovs, Anatolijs Kuzņcovs. Vēstures avoti ; 6 (Riga: Latvijas Valsts vēstures arcīva izdevniecība, 2009)

  15. Comprehensive Evaluation on Functions & Safety of Imported GM Soybean Using BDI-GS System%用BDI-GS体系综合评价进口转基因大豆的功效与安全

    Institute of Scientific and Technical Information of China (English)

    周则卫; 王德芝; 沈秀; 王浩; 白佳利; 王晓光; 龙伟

    2012-01-01

    In the present study,an animal model was established by feeding with maize low-nutrient diets,and the imported genetically modified ( GM) soybeans was evaluated through the novel BDI-GS system approaches established for functions and safety of food. The growing healthy ICR mice were selected as subject, the control mice were fed simply with maize diet, and the mice subjected test were fed with maize blending 15% conventionally natural soybean and 15% GM soybean. Through the whole duration of 13-day feeding test,obtained mice body weights,9 parameters of organ or tissue weights,indices and its BDI, GS, further analyzed the serum biochemical parameters as adjuvant evidences. The results show that RR soybean may thwart growth performance of test mice,obviously decreased BDI in part parameters and accumulation of general score( GS) . The different nutrition and health effects for RR and natural soybean mainly existed in organ parameters of thymus, pancreas and sper-mary. It manifests that imported GM soybean is worse in nutrition value and health functions than that of natural soybean produced in China,with some healthy risks in lowering immune and endocrine function,etc.%以低营养玉米饲料建立动物模型,通过已建立的食品BDI-GS评价新体系对进口抗草苷膦(RR)转基因(GM)大豆的功效安全进行评价.以处于生长期健康的ICR小鼠为研究对象,以单纯玉米饲料喂食小鼠作空白对照组,15%天然大豆及15%转基因大豆制成玉米掺和饲料为受试物实验组,通过13 d喂养,取得小鼠体重、9项脏器组织重量、系数及其BDI和GS等指标,并进行血清生化分析测试作为辅助评价指标.结果显示抗草苷膦转基因大豆喂养条件下,小鼠部分指标的BDI及累计GS明显降低;脏器指标主要表现在胸腺、胰腺和性腺的营养和健康效应上存在显著差异.表明进口GM大豆的营养价值及健康效应方面均明显不如国产天然大豆,存在一定的免

  16. Áföll af völdum sjálfsvígs og sjálfsvígstilrauna ungmenna. Forvarnir og aðstoð innan grunnskóla Hafnarfjarðar

    OpenAIRE

    Ægir Örn Sigurgeirsson 1970

    2012-01-01

    Rannsóknin fjallar um áfallahjálp í grunnskólum Hafnarfjarðar þegar áfall hefur orðið af völdum sjálfsvígs eða sjálfsvígshegðunar. Rannsóknin fjallar einnig um hvernig forvörnum gegn sömu þáttum er háttað. Markmiðin voru að skoða verkferla og leggja til viðbætur væri þess þörf. Framkvæmd var eigindleg viðtalsrannsókn með þátttöku kennara og starfsfólks áfallateyma úr fimm af sjö grunnskólum Hafnarfjarðar. Rannsóknin var unnin á haustmánuðum 2011. Niðurstöður rannsóknarinnar benda til að vel s...

  17. Comprehensive Evaluation on Nutritional and Healthy Effects of Peanut by Using BDI-GS System%用BDI-GS体系评价花生的营养健康效应

    Institute of Scientific and Technical Information of China (English)

    王德芝; 龙伟; 李卫红; 沈秀; 王浩; 周晓靓; 周则卫

    2015-01-01

    通过玉米饲料喂养的低营养动物模型,结合已建立的BDI-GS体系进行带衣花生营养健康效应的综合评价,用损益指数(BDI)及其累计积分(GS)考察花生对机体各重要脏器组织及微观血清生化指标的影响。以线性生长期健康的ICR小鼠为研究对象,分别设有玉米空白对照组,掺和8%、16%、24%带衣花生米组,喂养12天后解剖,完整剖取9项脏器组织,并进行血清生化指标的分析检测。结果显示,花生对机体胸腺、脾脏指标的营养功效显著,表现在3个剂量对全部脏器组织的重量BDI值均高于2.0;对其他脏器也具有良好的营养效应,表现在器官的重量BDI明显高于1.0。另外,花生对胸腺、脾脏、胰腺及性腺指标的健康存在显著有益效应;且综合营养、健康效应良好,体现在3个剂量花生实验组的累计GS均>9.0(9项指标)的基本积分。本研究表明,生理剂量下摄取花生对机体重要脏器组织具有很高的营养健康效应,对机体发育具有良好的促进效应。花生摄取对营养不良、亚健康及II型糖尿病会具有较好的预防及食疗保健功效,值得在日常饮食中特别重视,以及深入挖掘开发相关的健康食品。%Through low-nutritional mice model fed with maize diet combined the BDI-GS evaluation system established for food, nutritional and healthy effects of peanut with skin were evaluated comprehensively, observe the influences of its intake for functions of vital organs and/or tissues by using approach of benefit-damage index (BDI) and accumulative general scores (GS), as well as microscope serum biochemical parameters. The healthy ICR male mice during linear growth were used as subject. Including of maize fed control mice, 8%, 16%and 24%blending peanut kernel diets fed mice were designed in the study, respectively. After 12 days of breeding, recorded mice body weights and took blood samples

  18. In brown adipocytes, adrenergically induced β1-/β3-(Gs)-, α2-(Gi)- and α1-(Gq)-signalling to Erk1/2 activation is not mediated via EGF receptor transactivation

    International Nuclear Information System (INIS)

    Brown adipose tissue is unusual in that the neurotransmitter norepinephrine influences cell destiny in ways generally associated with effects of classical growth factors: regulation of cell proliferation, of apoptosis, and progression of differentiation. The norepinephrine effects are mediated through G-protein-coupled receptors; further mediation of such stimulation to e.g. Erk1/2 activation is in cell biology in general accepted to occur through transactivation of the EGF receptor (by external or internal pathways). We have examined here the significance of such transactivation in brown adipocytes. Stimulation of mature brown adipocytes with cirazoline (α1-adrenoceptor coupled via Gq), clonidine (α2 via Gi) or CL316243 (β3 via Gs) or via β1-receptors significantly activated Erk1/2. Pretreatment with the EGF receptor kinase inhibitor AG1478 had, remarkably, no significant effect on Erk1/2 activation induced by any of these adrenergic agonists (although it fully abolished EGF-induced Erk1/2 activation), demonstrating absence of EGF receptor-mediated transactivation. Results with brown preadipocytes (cells in more proliferative states) were not qualitatively different. Joint stimulation of all adrenoceptors with norepinephrine did not result in synergism on Erk1/2 activation. AG1478 action on EGF-stimulated Erk1/2 phosphorylation showed a sharp concentration–response relationship (IC50 0.3 µM); a minor apparent effect of AG1478 on norepinephrine-stimulated Erk1/2 phosphorylation showed nonspecific kinetics, implying caution in interpretation of partial effects of AG1478 as reported in other systems. Transactivation of the EGF receptor is clearly not a universal prerequisite for coupling of G-protein coupled receptors to Erk1/2 signalling cascades. - Highlights: • In brown adipocytes, norepinephrine regulates proliferation, apoptosis, differentiation. • EGF receptor transactivation is supposed to mediate GPCR-induced Erk1/2 activation. • α1-, α2-, β1

  19. The INTEGRAL long monitoring of persistent Ultra Compact X-ray Bursters

    OpenAIRE

    Fiocchi, M.; Bazzano, A.; Ubertini, P.; Bird, A. J.; Natalucci, L; Sguera, V.

    2008-01-01

    The combination of compact objects, short period variability and peculiar chemical composition of the Ultra Compact X-ray Binaries make up a very interesting laboratory to study accretion processes and thermonuclear burning on the neutron star surface. The improved large optical telescopes and more sensitive X-ray satellites have increased the number of known Ultra Compact X-ray Binaries allowing their study with unprecedented detail. We analyze the average properties common to all ultra comp...

  20. Galactic Gamma-Ray Bursters The Cosmic-Ray Sources at all Energies

    CERN Document Server

    Dar, Arnon

    1999-01-01

    We propose a new paradigm for the origin of nonsolar hadronic cosmic rays (CRs) at all energies: Highly relativistic, narrowly collimated jets from the birth or collapse of neutron stars in our Galaxy accelerate ambient disk and halo matter to CR energies and disperse it in hot spots which they form when they stop in the Galactic halo. Such events, seen as cosmological gamma-ray bursts in other galaxies when their beamed radiation happens to point towards Earth, account for many observed properties of the main hadronic component of CRs in a more natural way than all previously discussed CR sources.

  1. The Rapid Burster and its X-ray bursts: extremes of accretion and thermonuclear burning

    OpenAIRE

    Klis, van der, M.; Zand, in 't, J.J.M.; Watts, A.; Bagnoli, T.

    2015-01-01

    X-ray bursts originate from accreting neutron stars (NSs) in X-ray binaries (XRBs). They come in two flavours: thermonuclear bursts are due to the sudden runaway burning of the material accreted on the surface; accretion bursts signal a sudden change in the mass accretion rate, leading to enhanced emission in the innermost regions of the accretion flow. While thermonuclear bursts have been observed from 105 NSs as of writing, accretion bursts remain enigmatically confined to only two sources....

  2. Taxonomy of gamma ray burster data using a self-organizing neural network

    Energy Technology Data Exchange (ETDEWEB)

    Baumgart, C.W.

    1993-12-31

    This paper summarizes the results of a signal taxonomy study of gamma ray burst (GRB) data acquired with sensors on-board the Pioneer-Venus Orbiter (PVO) spacecraft. GRB events produce large fluxes of gamma rays with durations of seconds to minutes and have been observed since the early 1970`s. The true nature of GRBs is still unknown and several competing theories exist. A fundamental point of contention among such theories is whether or not different types of GRB exist. If different types of GRBs are discovered in the existing PVO data base, the differences may correlate with their position or source characteristics. Hence, the goal of this project was to use artificial neural networks to perform signal taxonomy on the GRB data base to determine if unique classes or types of GRBs exist. A total of 26 signal features were identified, some of which can be associated directly with some characteristic of the GRB, such as duration, peak count rate, and gamma ray spectrum hardness. Additional features that were selected included the number of zero crossings in the wavelet transform and the fractal dimension of each signal. A self organizing neural network was used with the signal features to search for correlations among the signals contained in the database. The results of this analysis revealed an intrinsic dimensionality of 2 or 3 in the database. That is, it appears as though 2 or 3 distinct types of GRB may exist. In particular, two of the classes contain roughly 90% of the signals in the database of GRB signals we had to work with. These two classes are similar in characteristics but are still sufficiently distinct from one another to form separate categories. The third class of GRB is definitely distinct from the first two.

  3. Codimension-two homoclinic bifurcations underlying spike adding in the Hindmarsh-Rose burster

    CERN Document Server

    Linaro, Daniele; Desroches, Mathieu; Storace, Marco

    2011-01-01

    The well-studied Hindmarsh-Rose model of neural action potential is revisited from the point of view of global bifurcation analysis. This slow-fast system of three paremeterised differential equations is arguably the simplest reduction of Hodgkin-Huxley models capable of exhibiting all qualitatively important distinct kinds of spiking and bursting behaviour. First, keeping the singular perturbation parameter fixed, a comprehensive two-parameter bifurcation diagram is computed by brute force. Of particular concern is the parameter regime where lobe-shaped regions of irregular bursting undergo a transition to stripe-shaped regions of periodic bursting. The boundary of each stripe represents a fold bifurcation that causes a smooth spike-adding transition where the number of spikes in each burst is increased by one. Next, numerical continuation studies reveal that the global structure is organised by various curves of homoclinic bifurcations. In particular the lobe to stripe transition is organised by a sequence ...

  4. Fireballs from Quark Stars in the CFL Phase: Application to Gamma Ray Bursters

    CERN Document Server

    Ouyed, R; Vogt, C

    2005-01-01

    Recent studies of photon-generation mechanisms in the color-superconducting Color-Flavor Locked (CFL) phase of dense quark matter have found gamma-ray emissivities in excess of ~ 10^{50} erg cm^{-3} s^{-1} for temperatures in the 10-30 MeV range. We suggest that this property can trigger gamma-ray bursts (GRBs) and associated fireballs at the surface of hypothetical hot (newly born) quark stars with an energy release of up to 10^{48}-10^{50} erg within a fraction of a millisecond. If surrounded by an accretion disk following its formation, the star's bursting activity can last from tens of milliseconds to hundreds of seconds releasing up to 10^{52} erg in total energy. We discuss typical features of observed GRBs within our model and explain how quark stars in the CFL phase might constitute natural candidates for corresponding inner engines.

  5. Canards in a minimal piecewise-linear square-wave burster

    Science.gov (United States)

    Desroches, M.; Fernández-García, S.; Krupa, M.

    2016-07-01

    We construct a piecewise-linear (PWL) approximation of the Hindmarsh-Rose (HR) neuron model that is minimal, in the sense that the vector field has the least number of linearity zones, in order to reproduce all the dynamics present in the original HR model with classical parameter values. This includes square-wave bursting and also special trajectories called canards, which possess long repelling segments and organise the transitions between stable bursting patterns with n and n + 1 spikes, also referred to as spike-adding canard explosions. We propose a first approximation of the smooth HR model, using a continuous PWL system, and show that its fast subsystem cannot possess a homoclinic bifurcation, which is necessary to obtain proper square-wave bursting. We then relax the assumption of continuity of the vector field across all zones, and we show that we can obtain a homoclinic bifurcation in the fast subsystem. We use the recently developed canard theory for PWL systems in order to reproduce the spike-adding canard explosion feature of the HR model as studied, e.g., in Desroches et al., Chaos 23(4), 046106 (2013).

  6. Swift follow-up of the newly discovered burster millisecond pulsar IGR J17511-3057

    DEFF Research Database (Denmark)

    Bozzo, E.; Ferrigno, C.; Kuulkers, E.; Falanga, M.; Chenevez, Jérôme; Brandt, Søren Kristian; Beckmann, V.; Bird, A.; Domingo, A.; Ebisawa, K.; Jonker, P.; Kretschmar, P.; Markwardt, C.; Oosterbroek, T.; Paizis, A.; Risquez, D.; Sanchez-Fernandez, C.; Shaw, S.; Wijnands, R.

    2.5 ks were accumulated in window timing (WT) mode. A thermonuclear type-I X-ray burst was discovered in the WT light curve of the source at 2009-09-14 00:51:37 UTC with an exponential decay time of ~12.5 s. This burst is most likely a He burst. The WT 0.5-10 keV X-ray spectrum is well described...

  7. 高分子量麦谷蛋白亚基分子标记在小麦品种改良中的应用%Application of Molecular Markers of HMW GS in Wheat Improvement

    Institute of Scientific and Technical Information of China (English)

    陈新民; 李思敏; 何中虎; 张艳; 夏先春; 邵凤成; 王德森; 张文祥; 王忠伟; 张勇; 张运宏

    2012-01-01

    为了同时对产量和品质进行改良,以面包小麦品种豫麦34为优质供体,与高产品种轮选987杂交,并结合高分子量麦谷蛋白亚基(HMW GS)7+8和5+10的分子标记鉴定,回交两次,育成12个同时含有7+8和5+10亚基的BC2F5品系,并以这12个品系为材料,研究了对轮选987品质和产量的改良效果。结果表明,9个品系的主要品质指标,如粉质仪形成时间、稳定时间,拉伸仪最大抗延阻力,和面仪和面时间、峰值面积、8 min带宽,面包体积和面包评分,均较轮选987明显增加,增幅依次为0.5~4.3 min、2.6~12.4 min、118.4~315.3 BU、0.7~3.5 min、23.6% TQ·min~134.9% TQ·min、6.1%~9.7%、80~165 cm、12.5~30分。1BL/1RS易位对品质负向影响较大,但也有例外。11个品系比轮选987增产,增幅为3.3%~19.5%,其中 CA1063、CA1062和CA1061三个品系达显著水平,且比对照中麦175分别增产4.1%、3.5%和1.9%;8个品系产量与中麦175差异不显著,品质明显优于轮选987和中麦175。这说明通过分子标记辅助选择可以实现产量与面包品质的同步改良。%To improve wheat yield and bread quality simultaneously, 12 BC2F5 breeding lines with high molecular glutein subunits (HMW GS) of 7+8 and 5+10, which derived from the cross between Yumai 34 and Lunxuan 9873 and identified by molecular marker assisted selection, were used to investigate the performance of yield and quality. The cultivar Yumai 34 with high bread making quality was used as the donor of HMW GS 7+8 and 5+10, and Lunxuan 987 as the high yield backcross parent. Results showed that 9 out of the 12 lines had higher values than Lunxuan 987 on most of the quality parameters, such as dough development time and stability of farinogragh, maximum resistance of extensogragh, mixing time, peak integral and Time X width at 8 minute of mixogragh, loaf volume and total score, which ranging from 0.5 to 4

  8. Expression of laccase lac2 gene of Bacillus subtilis in P.pastoris by high-density cell culture%工程菌P.pastoris GS115(pPIC9K-lac2)的构建及诱导表达漆酶的研究

    Institute of Scientific and Technical Information of China (English)

    沈锦城; 张泽华; 杨穗珊; 张添元; 罗进贤; 张爱联

    2013-01-01

    用PCR法扩增枯草芽孢杆菌的漆酶基因lac2.构建表达质粒pPIC9K-lac2.通过电转法将lac2基因重组于P.pastoris基因组,筛选高G418抗性和高表达漆酶的转化子作为工程菌GS115(pPIC9K-lac2).在发酵罐中发酵GS115(pPIC9K-lac2)表达重组蛋白.在50 L发酵罐中加入20 L无机盐发酵培养基.在发酵的第一阶段连续24 h补加50%甘油-0.8% PTM4增殖P.pastoris,然后用甲醇-0.8% PTM4诱导49 h.在发酵过程中,通过调节搅拌的频率和通气量,将溶氧维持于20% ~30%,用氨水维持pH 5.0.放罐时生物量为A600=266.5,表达漆酶1097.5U/L发酵液.%The gene of lac2 was amplified from the Bacillus subtilis chromosome by PCR technique according to the pub?lished sequence of lac2 and cloned directly into the P. pastoris vector pPIC9K, resulted in the expression plasmid pPIC9K-Zac2 which was then transformed into P. pastoris GS115 by electroporation method. A recombinant transformant with high G418 resistant characteristics and well expression was selected as engineering strain GS115 ( pPIC9K-Zac2). The fermentation was carried out in a 50 L bioreactor with 20 L inorganic salt fermentation medium. The cells were first grown in 50% glycerol -0. 8% PTM4 trace salts for 24 h and then induced by methanol -0.8% PTM4 for 49 h. During the process of the fermentation, the dissolved oxygen was maintained between 20% -30% by adjusting the rates of agitation and aeration, and pH was controlled at 5 by NH40H. At the end of the fermentation, the biomass growth was 266. 5 as measured by absorption of 600nm, while expressed laccase was 1097.5U/L fermentation broth.

  9. 三七总皂苷对大鼠脊髓损伤后GS的表达及运动功能恢复的影响%Effect of tPNS on GS expression and motor function recovery after spinal cord injury in rats

    Institute of Scientific and Technical Information of China (English)

    李花; 陈安; 李亮; 廖君; 邓常清

    2012-01-01

    Objective To investigate the effect of total panax notoginseng saponins(Tpns)on motor function recovery after semi-transverse spinal cord injury and its association with expression of glutaminesynthetase(CS). Methods The rats were randomly divided into normal group, vehicle control group and Tpns group, the rats in experimental group were induced the spinal cord hemisection model, in TPNS group the spinal cord injury was at the right side of the T10th segment. After injury 15 min, dose of 20 mg/kg of PNS were injected into the abdominal cavity, once daily; in vehicle control group injected with normal saline. The target behaviors were detected at 1st, 3rd, 7th, 14th, 21st and 28th day after operaection; the GS expression changes of spinal cord injury in far side were detected with immunohistochemical detection. Results Behaviors showed that Tpns at the this concentration significantly promote the recoeryof motor function, in which, at 7th and 14th day after injury, the BBB cores showed that the motor function recovery in Tpns group was significantly higher than that in vehicle control group; the immunohistochemistry results showed that the spinal cord hemisection injury in the distalspinal cord injury the expression of CS was stronger than the contralateral side of injury; the GS expression tends in the damage side were increased at 1st to 3rd day gradually, and reached the peak at 7th day, after 14th day, decreased gradually and to 28th day was still slightly higher than the normal group. Compared with vehicle control group, CS expression in PNS group at the same time stronger than that in control group, especially in the 3rd and 7th day. Conclusion Promoting recovery of motor function of spinal injury in rats, the machnism of Tpns may be associated with the increased GS expressive to improve the microenvironment for spinal cord regeneration.%目的 探讨三七总皂苷(total panax notoginseng saponins,TPNS)对大鼠脊髓半横断损伤后运动功能恢复的

  10. Behind the scenes of GS: CERN’s beating heart

    CERN Multimedia

    Antonella Del Rosso

    2014-01-01

    Founded at the same time as CERN, the library has followed, and sometimes even moved ahead of, the changes in the Organization. Today, far from being a simple book depository, the Scientific Information Service (SIS) that manages CERN's library is increasingly digitising its material and investing in innovative projects, such as Open Access.   Ever since it was set up in Building 52 on 1 September 1957, the library has played a vital role in the Organization.  “Our only official task is to provide a complete list of the publications by CERN researchers, but, in fact, this place is the memory bank of the whole Laboratory,” emphasises Tullio Basaglia, head of the Library Section within SIS.  “The role of the library and archives is to preserve, document and disseminate the knowledge produced at CERN.” Sixty years after its creation, the library today contains 90,000 books, two thirds of which are available in a digital ...

  11. The attached algae community near Pickering GS: III

    International Nuclear Information System (INIS)

    The relationship between attached algae and macro-invertebrates in the nearshore zone of Lake Ontario was investigated in the vicinity of the Pickering 'A' NGS. Measures of faunal density, richness, evenness, and biomass were generally higher from areas which supported attached algae. Gammarus fasciatus, Cricotopus bicinctus, Dicrotendipes spp., Orthocladius obumbratus, Cladotanytarsus spp., Orthocladius spp., and Parakiefferiella spp., were significantly correlated with algal standing crop. All of the above dominant invertebrates ingested epiphytes associated with Cladophora glomerata. Attempts to explain the distribution of the zoobenthic assemblages using the physical/biological characteristics of the study area indicated algal cover, substrate size, wind velocity and water temperature were most important

  12. Behind the scenes of GS: cross-platform

    CERN Multimedia

    Katarina Anthony

    2014-01-01

    The year was 1989: the dawn of administrative computing. In a laboratory filled to the rafters with paperwork, CERN's then Director-General Carlo Rubbia saw an opportunity for a complete administrative overhaul. He established the Advanced Information Systems (AIS) project to analyse CERN's administration, which in turn suggested the Electronic Document Handling (EDH) system. By 1992, EDH was up and running - the start of a new chapter in CERN history.   If you think you've never come accross EDH, think again. The system is an integral part of CERN life, handling everything from the purchase of materials to leave requests. EDH sees you through your entire CERN life: from your first CERN job application to your final retirement checklist. One platform, sixty-five functions What makes EDH so special is its solitary nature: it is one platform that carries out dozens of varied functions. "Most companies organise their administration in 'vertical' ...

  13. SSCI 125 emulation of Point Lepreau G.S

    International Nuclear Information System (INIS)

    A C language program has been developed at the Point Lepreau Nuclear Generating Station which emulates the actions of an SSCI 125 computer's instruction set and also emulates the actions of those input and output devices which belong to the plant control computer of the nuclear power plant's full scope training simulator. This enables the same DCC software to run on both the $20 million dollar training simulator and an equivalent desktop simulator whose hardware cost is now $16,000 or less. It facilitates making additional copies of the $20 million dollar training simulator for less than 0.1% of the cost per copy. When this emulation program is run on a 266 MHz DEC single CPU Alpha computer, it simulates the running of the training simulator plant control DCC (digital control computer) at approximately the rate of real time: either faster or slightly slower depending on the compiler options employed and depending on the choice of the modeled machine cycle speed of the SSCI. Using the standard machine cycle speed of 660 nanoseconds, using the emulator fully optimized and using plant process modelling without optimization the speed is 90% as fast as real time - this is the normal use situation for simulator modelers who were the originally intended users of the emulator. If the modeled SSCI machine cycle speed is slowed down to 792 nanoseconds (making use of space capacity on the real SSCI to reduce the workload of emulation), and all objects are compiled as optimized, then it takes only about 20 minutes of clock time to simulate over 30 minutes of modelling time. This mode of simulation may be useful for somebody who is not doing emulator or modeling code development. This could include preparing lesson plans for the training simulator, developing or evaluating plant operating procedures or trying out revised DCC software. (DM)

  14. Behind the scenes of GS: health in the workplace

    CERN Multimedia

    CERN Bulletin

    2014-01-01

    CERN has had its own Medical Service since 1965. Open to members of the personnel, users and visitors, the team offers care and advice with professionalism and commitment.   The Medical Service team, currently consisting of three doctors, four nurses, a lab technician, a psychologist and two administrative assistants, provides an ever-increasing number of consultations. In 2013, around 7800 people were seen by the Medical Service, mainly at walk-in consultations, and more than 6665 further tests were performed (e.g. electrocardiograms, hearing tests, lung function tests, urine tests, blood tests). While its chief objective is to monitor the health of the CERN community in relation to their work through regular medical check-ups, the Medical Service also performs an important primary care function: “More than 10,000 people work in CERN’s offices, workshops, tunnels and worksites every day. As in any community of that size, sometimes certain situations arise that demand imme...

  15. Behind the scenes of GS: Mapping the future of CERN

    CERN Multimedia

    Sophie Louise Hetherton

    2014-01-01

    Focus on the Future Accelerator Studies (FAS) section at CERN who carry out the civil engineering studies for the Laboratory’s future scientific facilities.   The Future Accelerator Studies (FAS) section co-ordinates the civil engineering and infrastructure studies for large-scale future physics projects. These include projects due to complete in a couple of years such as AWAKE, but also projects planned for ten years’ time such as the High Luminosity LHC (HL-LHC) or even longer term projects such as the Future Circular Collider (FCC), for which approval is still pending. “CERN needs to be able to study the feasibility and assess the risks of future projects, even though they are not all fully approved,” explains John Osborne, Head of the FAS section, which also includes two fellows and one technical student. “Our small team works closely with other groups across CERN and with external companies to provide studies from a civil engineering poin...

  16. Point Lepreau G.S. commissioning management experience

    International Nuclear Information System (INIS)

    This paper shares the experience gained by New Brunswick Power in commissioning its first nuclear plant, with particular emphasis on the management techniques that were developed. Some of the areas discussed include: organization of Nuclear Operations Group responsible for commissioning and its interface with construction; outline and scope of documentation required to define, execute and report commissioning to satisfy the licensing requirements, management techniques developed to implement commissioning; and a summary of lessons learned and recommendations for the future

  17. Molecular Characterization of 2-Chlorobiphenyl Degrading Stenotrophomonas maltophilia GS-103

    Digital Repository Service at National Institute of Oceanography (India)

    Somaraja, P.K.; Gayathri, D.; Ramaiah, N.

    of each primer, 1.25 U of Taq polymer- ase (Sigma Aldrich, Germany), and *10 ng of DNA template. PCR amplifications were performed in T-gradient thermal cycler (Biometra, Goettingen, Germany) pro- grammed as follows: 5 min of denaturation at 95�C, fol...

  18. Innovative approach to replace concrete aqueducts at Kakabeka Falls GS

    Energy Technology Data Exchange (ETDEWEB)

    Chidiac, M. [Stone and Webster Canada, Toronto, ON (Canada); Dunphy, J. [Aker Maritime Kiewit Contractors, St. John' s, NF (Canada); Young, L. [Exebridge Project Services, Toronto, ON (Canada)

    2004-09-01

    The original facilities of the Kakabeka Falls generating station on the Kaministikwia River west of Thunder Bay, Ontario were constructed between 1906 and 1914. They included 3 low pressure, reinforced concrete aqueducts that measured 3.1 meter in diameter and 1980 meters long to carry water to a powerhouse containing 4 generators. In 1994, the aqueducts were deemed to be at the end of their service life and required replacement to avoid a failure which would potentially cause serious environmental damage, pose a threat to public safety and result in a severe loss of generation production. Several alternative water transport systems were considered to replace the aqueducts. One single 5 meter diameter aqueduct made of cast-in-place reinforced concrete was chosen to replace the 3 aging aqueducts. Replacement of the aqueduct began in May 1997 and work was completed on schedule 7 months later. The generating station returned to service in early 1998. The new aqueduct has performed excellently during its 6 years in service and does not show any evidence of concrete deficiencies such as cracks or erosions. Some minor repairs were made to the elastomeric seal membrane at a few joints where some delamination occurred but with no leakage path developing. The new aqueduct is expected to last nearly 100 years. 12 figs.

  19. Full load rejection procedure for Point Lepreau GS

    International Nuclear Information System (INIS)

    In 1999 WANO issued Significant Operating Event Report 99-01 recommending that the industry verify whether or not currently approved procedures were adequate for a loss or degradation of the electrical grid. A detailed review identified that the procedures available at the time were not adequate to respond effectively to such a disturbance. Operations personnel reviewed operating expectations and the expected plant response to a Loss of Grid and updated the Operating Manual to cover 'Full Load Rejection'. Operations used the simulators to validate the procedure and train crews and Licensed staff. The revised documentation was approved and implemented. (author)

  20. Gs Nanotech приближает будущее: важный шаг для развития отечественной потребительской электроники сделан

    OpenAIRE

    2015-01-01

    В 2014 году предприятие GS Nanotech (ОАО ДжиЭс-Нанотех) первым в России приступило к массовому выпуску многокристального микропроцессора коммерческого назначения по технологии SiP (SysteminPackage, система-в‑корпусе) GS Nanotech SiP Amber S2. Устройство было полностью разработано R&D‑подразделением компании. Это событие можно назвать важным шагом на пути развития отечественной потребительской электронной индустрии....

  1. Natural analogue synthesis report, TDR-NBS-GS-000027 rev00 icn02

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, A.; Nieder-Westermann, G.; Stuckless, J.; Dobson, P.; Unger, A.J.A.; Kwicklis, E.; Lichtner, P.; Carey, B.; Wolde, G.; Murrel,M.; Kneafsey, T.J.; Meijer, A.; Faybishenko, B.

    2002-04-01

    The purpose of this report is to present analogue studies and literature reviews designed to provide qualitative and quantitative information to test and provide added confidence in process models abstracted for performance assessment (PA) and model predictions pertinent to PA. This report provides updates to studies presented in the Yucca Mountain Site Description (CRWMS M&O 2000 [151945], Section 13) and new examples gleaned from the literature, along with results of quantitative studies conducted specifically for the Yucca Mountain Site Characterization Project (YMP). The intent of the natural analogue studies was to collect corroborative evidence from analogues to demonstrate additional understanding of processes expected to occur during postclosure at a potential Yucca Mountain repository. The report focuses on key processes by providing observations and analyses of natural and anthropogenic (human-induced) systems to improve understanding and confidence in the operation of these processes under conditions similar to those that could occur in a nuclear waste repository. The process models include those that represent both engineered and natural barrier processes. A second purpose of this report is to document the various applications of natural analogues to geologic repository programs, focusing primarily on the way analogues have been used by the YMP. This report is limited to providing support for PA in a confirmatory manner and to providing corroborative inputs for process modeling activities. Section 1.7 discusses additional limitations of this report. Key topics for this report are analogues to emplacement drift degradation, waste form degradation, waste package degradation, degradation of other materials proposed for the engineered barrier, seepage into drifts, radionuclide flow and transport in the unsaturated zone (UZ), analogues to coupled thermal-hydrologic-mechanical-chemical processes, saturated zone (SZ) transport, impact of radionuclide release on the biosphere, and potentially disruptive events. Results of these studies will be used to corroborate estimates of the magnitude and limitation of operative processes in order to build realism into conceptual and numerical process models used as a foundation for PA in the representative case of postclosure safety.

  2. Implementation of City Gs Distribution Project in Wajo Regency, South Sulawesi

    OpenAIRE

    Amaliyah, Novriany

    2011-01-01

    Natural gas is a major source of electricity generation through the use of gas turbines and steam turbines. Natural gas burns more cleaner than other hydrocarbon fuels, such us oil and coal and produces less carbon dioxide per unit of energy release. About 20 years, Energy Equity Epic Pty. Ltd as contractor sharing with PERTAMINA has been explored natural gas in Wajo Regency, South Sulawesi as a source of electricity generator. As a result of its environmentally friendliness and stable ...

  3. Parkinson's disease (PD) in the elderly: an example of geriatric syndrome (GS)?

    Science.gov (United States)

    Lauretani, Fulvio; Maggio, Marcello; Silvestrini, Claudio; Nardelli, Anna; Saccavini, Marsilio; Ceda, Gian Paolo

    2012-01-01

    PD is an age-related neurodegenerative disorder that affects as many as 1-2% of persons aged 60 years and older. In the latest decade, the approach to PD was dramatically changed. In fact, although for many years PD has been considered only "a disease that affects walking", with a key role of the neurotransmitter dopamine, recently the neurological approach has been substantially modified. The approach for this disease is not only a neurological issue. Given the complexity of its clinical aspects, such as depression, anxiety, dementia, sleep disorder, pneumonia dysfagia-related and malnutrition, a multidisciplinary evaluation and not just a neurological evaluation is needed. We suggest a n multidisciplinary approach for this old actor, underlying a subtle link between neurophatological stages of the disease (Braak's classification) and clinical aspects (Braak's stages 1 and 2 associated with the premotor phase; Braak's stages 3-4 associated with the motor symptoms and Braak's stages 5-6 associated with cognitive impairment). In addition, we emphasize the usefulness of geriatric evaluation for the identification of frail "in situ", frail, and disable status for improving care and treatment in this multifaceted disease. PMID:21459464

  4. Crystal structure of the β2 adrenergic receptor-Gs protein complex

    DEFF Research Database (Denmark)

    Rasmussen, Søren Gøgsig Faarup; DeVree, Brian T; Zou, Yaozhong; Kruse, Andrew C; Chung, Ka Young; Kobilka, Tong Sun; Thian, Foon Sun; Chae, Pil Seok; Pardon, Els; Calinski, Diane; Mathiesen, Jesper M; Shah, Syed T A; Lyons, Joseph A; Caffrey, Martin; Gellman, Samuel H; Steyaert, Jan; Skiniotis, Georgios; Weis, William I; Sunahara, Roger K; Kobilka, Brian K

    2011-01-01

    G protein-coupled receptors (GPCRs) are responsible for the majority of cellular responses to hormones and neurotransmitters as well as the senses of sight, olfaction and taste. The paradigm of GPCR signalling is the activation of a heterotrimeric GTP binding protein (G protein) by an agonist...

  5. Essential Competencies for Interdisciplinary Graduate Training in IGERT: Final Report. GS-10F-0086K

    Science.gov (United States)

    Gamse, Beth C.; Espinosa, Lorelle L.; Roy, Radha

    2013-01-01

    The Integrative Graduate Education and Research Traineeship (IGERT) program represents a substantial investment by the National Science Foundation (NSF) to improve the quality of graduate education, and ultimately, to increase the number of graduates better prepared to address the nation's 21st century scientific and technological needs. The…

  6. AECB staff annual report of Point Lepreau G.S. for the year 1990

    International Nuclear Information System (INIS)

    This report is a review by AECB staff of the operation of Point Lepreau Nuclear Generating Station during 1990. The review is based on information contained in the various documents submitted by New Brunswick Power under the terms of the Operating Licence and on information gathered by AECB staff during routine site monitoring, inspections and audits

  7. Essential Competencies for Interdisciplinary Graduate Training: Summary Report. GS-10F-0086K

    Science.gov (United States)

    Gamse, Beth C.; Espinosa, Lorelle L.; Roy, Radha

    2013-01-01

    The Integrative Graduate Education and Research Traineeship (IGERT) program represents a substantial investment by the National Science Foundation (NSF) to improve the quality of graduate education, and ultimately, to increase the number of graduates better prepared to address our nation's 21st century scientific and technological needs. The…

  8. Chronically increased Gsα signaling disrupts associative and spatial learning

    OpenAIRE

    Bourtchouladze, Rusiko; Patterson, Susan L.; Kelly, Michele P.; Kreibich, Arati; Kandel, Eric R.; Abel, Ted

    2006-01-01

    The cAMP/PKA pathway plays a critical role in learning and memory systems in animals ranging from mice to Drosophila to Aplysia. Studies of olfactory learning in Drosophila suggest that altered expression of either positive or negative regulators of the cAMP/PKA signaling pathway beyond a certain optimum range may be deleterious. Here we provide genetic evidence of the behavioral and physiological effects of increased signaling through the cAMP/PKA pathway in mice. We have generated transgeni...

  9. Fermi Large Area Telescope Observations of the Supernova Remnant GS.7-0.1

    Science.gov (United States)

    Ferrara, E. C.; Hays, E.; Troja, E.; Moiseev, A. A.

    2012-01-01

    We present a detailed analysis of the GeV gamma-ray emission toward the supernova remnant (SNR) G8.7-0.1 with the Large Area Telescope (LAT) onboard the Fermi Gamma-ray Space Telescope. An investigation of the relationship among G8.7-0.1 and the TeV unidentified source HESS J1804-216 provides us with an important clue on diffusion process of cosmic rays if particle acceleration operates in the SNR. The GeV gamma-ray emission is extended with most of the emission in positional coincidence with the SNR G8.7-0.1 and a lesser part located outside the western boundary of G8.7-0.1. The region of the gamma-ray emission overlaps spatially-connected molecular clouds, implying a physical connection for the gamma-ray structure. The total gamma-ray spectrum measured with LAT from 200 MeV-100 GeV can be described by a broken power-law function with a break of 2.4 +/- 0.6 (stat) +/- 1.2 (sys) GeV, and photon indices of2.10 +/- 0.06 (stat) +/- 0.10 (sys) below the break and 2.70 +/- 0.12 (stat) +/- 0.14 (sys) above the break. Given the spatial association among the gamma rays, the radio emission ofG8.7-0.1, and the molecular clouds, the decay of pions produced by particles accelerated in the SNR and hitting the molecular clouds naturally explains the GeV gamma-ray spectrum. We also find that the GeV morphology is not well represented by the TeV emission from HESS Jl804-2l6 and that the spectrum in the Ge V band is not consistent with the extrapolation of the TeV gamma-ray spectrum. The spectral index of the TeV emission is consistent with the particle spectral index predicted by a theory that assumes energy-dependent diffusion of particles accelerated in an SNR. We discuss the possibility that the TeV-spectrum originates from the interaction of particles accelerated in G8.7-0.l with molecular clouds, and we constrain the diffusion coefficient of the particles.

  10. Effect of High Molecular Weight Glutenin Subunits (HMW-GS) on Tortilla Quality

    Science.gov (United States)

    Tortillas are the most popular non-bread wheat based product. Flour used in tortilla production has been typically optimized for bread making. The flour properties that determine good quality bread do not necessarily provide good quality tortillas. In this study, the influence of high molecular wei...

  11. Characterization of the metal binding histidine pockets at the surface of mammalian IgGs

    Czech Academy of Sciences Publication Activity Database

    Tishchenko, Galina; Hašek, Jindřich; Strachota, Adam; Skálová, Tereza; Buchtelová, Eva; Kurková, Dana; Brynda, Eduard; Štouračová, Renata

    Compiegne : University of Technology, 2003. s. 49. [International Symposium on Polymer Design for BioSeparation and Nanobiotechnology /8./. 27.11.2003-29.11.2003, Compiegne] R&D Projects: GA AV ČR KSK4055109 Institutional research plan: CEZ:AV0Z5052915; CEZ:AV0Z4050913 Keywords : mammalian immunoglobulin Subject RIV: CC - Organic Chemistry

  12. Gs-veg, fv. 325 Gunnerødbrekka, Horten kommune

    OpenAIRE

    Volden, Sindre; Haugsvold, Sverre; Moen, Tone; Kalleberg, Amanda Kristine

    2015-01-01

    Oppgaven er å se på nye løsninger for gående og syklende langs den 1,7 km eksisterende gang- og sykkelvegen langs fylkesveg 325 fra Bakkenteigen til Borre. Målet er å øke sikkerheten, fremkommeligheten og tryggheten til både gående og syklende. I denne oppgaven er det vurdert fire alternativer. På bakgrunn av verktøy som spørreundersøkelse, brukerintervju, SFT (sikkerhet, fremkommelighet, trygghet) og faglig kompetanse, er det gjennomført en analyse. Konklusjonen bygger på analysen som vis...

  13. A study of stress in medical students at Seth G.S. Medical College.

    OpenAIRE

    Supe A

    1998-01-01

    BACKGROUND: It is usually observed that medical students undergo tremendous stress during various stages of the MBBS course. There is a high rate of suicide among them. METHODS: To determine incidence of stress and factors controlling stress in medical students at various stages of MBBS course at Seth G S Medical college, 238 students (First year 98, Second 76, Third 64) were asked to complete a questionnaire on personal data (gender, stay at hostel, mode of travel, time spent in travel every...

  14. Optimization of a Modified GS Medium for a Probiotic Strain (L. acidophilus ATCC4356

    Directory of Open Access Journals (Sweden)

    N. Pedram

    2014-09-01

    Full Text Available Probiotics are defined as living microorganisms with beneficial effects on the host. Probiotics, according to the least negative effect on the body, are considered as a good alternative to chemical drugs. Lactobacillus acidophilus is used as a probiotic that is able to reduce cholesterol level in the blood. The effect of various concentrations of carbon, nitrogen and phosphorus for enhancing the biomass production of Lactobacillus acidophilus was examined. The response surface methodology based on Box–Wilson CCD was applied to explore the optimal medium composition. Glucose, yeast extract, K2HPO4 and KH2PO4 were selected as dependent variables. All experiments were run at 37°C for 31h under stationary conditions. By solving the regression equation and analyzing the response surface carton, optimal concentrations of the components were determined as glucose (5-8.75 g.l-1, yeast extract (36.75-39 g.l-1, K2HPO4 (0.1-0.2125 g.l-1 and KH2PO4 (0.3925-0.7075 g.l-1. Validation experiment confirmed that the optimized medium was comparable to the MRS medium (the most common medium for Lactobacillus acidophilus strain in biomass production, having the advantages of economy andpracticality.

  15. An upset response strategy for the Point Lepreau G.S. CANDU 600

    International Nuclear Information System (INIS)

    The Emergency Operating Procedures Standards for Canadian Nuclear Utilities specify the requirement for the development and use of a 'Response Strategy to Upsets'. A Response Strategy organizes and prioritizes the decisions and actions to be taken by the control room operating staff during any plant upset. This paper describes the purpose, design rationale, and application of the PLGS Upset Response Strategy. The development of a strategy requires a full understanding of the plant behaviour under upset conditions and an appreciation of human factors issues. Thus the strategy must consider: i) plant response time constants, ii) human response time constants, iii) priority order of EOPs and other corrective procedures, iv) the diagnostic capabilities of the operator, v) manpower availability in the control room and the field. The key to developing the PLGS Upset Response Strategy was in determining a priority order to operations tasks. The priority ranking recognizes the time constants associated with the unit response to upsets, and the required operator response ('human time constants'). This was established from a knowledge of simulator and safety report event response and observation of operator response during simulator-based upset training. The paper describes the PLGS Operator Upset Response Strategy and Shift Supervisor Upset Response Strategy logic and design rationale in detail. Both the management of plant upsets and upset management training benefit from using the upset strategy. The major benefit comes from providing a systematic, prioritized logic framework. (author) 4 refs., 1 tab., 3 figs

  16. A 4 GS/sec Instability Feedback Processing System for Intra-bunch Instabilities

    CERN Document Server

    Dusatko, J; Fox, J D; Pollock, K; Rivetta, C H; Turgut, O; Hofle, W

    2013-01-01

    We present the architecture and implementation overview of a proof-of-principle digital signal processing system developed to study control of Electron-Cloud and Transverse Mode Coupling Instabilities (TMCI) in the CERN SPS. This system is motivated by intensity increases planned as part of the High Luminosity LHC upgrade. It is based on a reconfigurable processing architecture which samples intra-bunch motion and applies correction signals at a 4GSa/s rate, allowing multiple samples across a single 3.2ns SPS bunch. This initial demonstration system is a rapidly developed prototype consisting of both commercial and custom- designed hardware that implements feedback control on a single bunch. It contains a high speed ADC and DAC, capable of sampling at up to 4GSa/s, with a 16-tap FIR control filter for each bunch sample slice. Other system features include a timing subsystem to synchronize the sampling to the injection and the bunch 1 markers, the capability of generating arbitrary time domain signals to drive...

  17. The role of global data identification standards for supply chain visibility: the case of GS1

    OpenAIRE

    Semianiaka, Natallia; Silina, Ekaterina

    2012-01-01

    Purpose. Supply chain visibility is among the top concerns expressed by many supply chain leaders. While the traditional enablers of supply chain visibility are information sharing, IT implementation, and relationships between supply chain partners; the role of global supply chain standards as an integral part of each of these enablers is often underestimated. The purpose of this thesis is to investigate the adoption and applicability of global supply chain standards within the provisions of ...

  18. AECB staff annual report on Point Lepreau G.S. for the year 1988

    International Nuclear Information System (INIS)

    AECB staff considers that the station has been operated and managed in a safe manner during 1988. The staff is satisfied that the NBEPC is proceeding properly towards resolution of any ongoing or outstanding work identified in this report

  19. A study of stress in medical students at Seth G.S. Medical College.

    Directory of Open Access Journals (Sweden)

    Supe A

    1998-01-01

    Full Text Available BACKGROUND: It is usually observed that medical students undergo tremendous stress during various stages of the MBBS course. There is a high rate of suicide among them. METHODS: To determine incidence of stress and factors controlling stress in medical students at various stages of MBBS course at Seth G S Medical college, 238 students (First year 98, Second 76, Third 64 were asked to complete a questionnaire on personal data (gender, stay at hostel, mode of travel, time spent in travel every day, medium of study in school, place of school education., Stress inducing factors, Zung′s depression scale, ways of coping, stress relievers, perceived social support and personality type. Statistical tests used were ANOVA, critical ratio and Student′s ′t′ test. RESULTS: Majority of medical students (175/238--73% perceived stress. Stress was found to be significantly more in Second and Third MBBS students rather than First MBBS levels (p < 0.05. Stress was not found to differ significantly on the basis of sex, stay at hostel, model of travel, time spent in travel every day, medium of study in school, place of school education. Stress was found to be significantly more in students having more than 95% of marks at 12th Standard as compared to others. Academic factors were greater perceived cause of stress in medical students. There was no significant difference in the students at different levels of MBBS regarding academic factors and social factors as a stress inducing factors. Physical factors were found to be significantly more in Second and Third MBBS students as compared to First MBBS students. Emotional factors were found to be significantly more in First MBBS students as compared to Second & Third MBBS students. Stress was more common in medical students who have dominant strategy of coping as positive reappraisal, accepting responsibility and planful problem solving. Stress was less common in medical students at Seth G S Medical College who have dominant strategy of coping as escaping and distancing from difficult situation. Family and Friend as perceived social supports were more in Second MBBS than First MBBS medical students. Stress was not found to be significantly more in students having their personality factor contributing to stress (Type A--52/67 as compared to others (Type B--123/171. This indicates that the stress was not trait oriented but was process oriented (p = NS. CONCLUSION: Stress in medical students is common and is process oriented. It is more in second and third year. Academic factors are greater perceived cause of stress in medical students at Seth G S medical college. Emotional factors are found to be significantly more in First MBBS. It is dependent on person′s ways of coping and social support.

  20. Natural analogue synthesis report, TDR-NBS-GS-000027 REV00 ICN02

    International Nuclear Information System (INIS)

    The purpose of this report is to present analogue studies and literature reviews designed to provide qualitative and quantitative information to test and provide added confidence in process models abstracted for performance assessment (PA) and model predictions pertinent to PA. This report provides updates to studies presented in the Yucca Mountain Site Description (CRWMS M and O 2000 [151945], Section 13) and new examples gleaned from the literature, along with results of quantitative studies conducted specifically for the Yucca Mountain Site Characterization Project (YMP). The intent of the natural analogue studies was to collect corroborative evidence from analogues to demonstrate additional understanding of processes expected to occur during postclosure at a potential Yucca Mountain repository. The report focuses on key processes by providing observations and analyses of natural and anthropogenic (human-induced) systems to improve understanding and confidence in the operation of these processes under conditions similar to those that could occur in a nuclear waste repository. The process models include those that represent both engineered and natural barrier processes. A second purpose of this report is to document the various applications of natural analogues to geologic repository programs, focusing primarily on the way analogues have been used by the YMP. This report is limited to providing support for PA in a confirmatory manner and to providing corroborative inputs for process modeling activities. Section 1.7 discusses additional limitations of this report. Key topics for this report are analogues to emplacement drift degradation, waste form degradation, waste package degradation, degradation of other materials proposed for the engineered barrier, seepage into drifts, radionuclide flow and transport in the unsaturated zone (UZ), analogues to coupled thermal-hydrologic-mechanical-chemical processes, saturated zone (SZ) transport, impact of radionuclide release on the biosphere, and potentially disruptive events. Results of these studies will be used to corroborate estimates of the magnitude and limitation of operative processes in order to build realism into conceptual and numerical process models used as a foundation for PA in the representative case of postclosure safety

  1. MASTER optical polarization variability detection in the Microquasar V404 Cyg/GS2023+33

    CERN Document Server

    Lipunov, Vladimir M; Kornilov, V; Krushinskiy, V; Vlasenko, D; Tiurina, N; Balanutsa, P; Kuznetsov, A; Budnev, N; Gress, O; Tlatov, A; Lopez, R Rebolo; Serra-Ricart, M; Buckley, D A H; Israelian, G; Lodieu, N; Ivanov, K; Yazev, S; Sergienko, Yu; Gabovich, A; Yurkov, V; Levato, H; Saffe, C; Podesta, R; Mallamaci, C; Lopez, C

    2016-01-01

    On 2015 June 15 the Swift space observatory discovered that the Galactic black hole candidate V404 Cyg was undergoing another active X-ray phase, after 25 years of inactivity (Barthelmy et al. 2015). Twelve telescopes of the MASTER Global Robotic Net located at six sites across four continents were the first ground based observatories to start optical monitoring of the microquasar after its gamma-ray wakeup at 18h 34m 09s U.T. on 2015 June 15 (Lipunov et al. 2015). In this paper we report, for the first time, the discovery of variable optical linear polarization, changing by 4-6% over a timescale of approximately 1 h, on two different epochs. We can conclude that the additional variable polarization arisies from the relativistic jet generated by the black hole in V404Cyg. The polarization variability correlates with optical brightness changes, increasing when the flux decreases.

  2. An Optimized Secure Communication Using VLR-GS-BU on Wireless Network

    Directory of Open Access Journals (Sweden)

    M.Saranya

    2012-07-01

    Full Text Available A novel protocol to achieve privacy-preserving universal authentication protocol for wireless communications called Priauth. to secure the communication as the data are sensitive or it requires the users to pay for it. In the algorithms for secure communication a key is shared by H with V and V with users. The key is used to encrypt data transmitted to the servers or users. In privacy based authentication protocols besides the user and its home server, no one including the foreign server can tell the identity of the user; and besides the user and its home server, no one including the foreign server is able to link any past or future protocol runs of the same user. An To revoke multiple users is to associate a key with everynonempty subset of users in the group. Thus, if one or more users are revoked, the VA uses the key associated with the subset of the remaining users to encrypt the new key and transmits the new group keyto them. The advantage of this approach is that the communication overhead is only one message for revoking any number of users. However, the number of keys stored by the VA and the users isexponential in the size of the group. The goal of the enhancement is to evaluate trade-off between storage and revocation cost. Storage is computed in terms of keys that each user (respectively, VA maintains.And revocation cost is computed in terms of the encryptions performed, and the number of messages transmitted, by the VA.

  3. An Optimized Secure Communication Using VLR-GS-BU on Wireless Network

    OpenAIRE

    Saranya, M; P.Dhivya

    2012-01-01

    A novel protocol to achieve privacy-preserving universal authentication protocol for wireless communications called Priauth. to secure the communication as the data are sensitive or it requires the users to pay for it. In the algorithms for secure communication a key is shared by H with V and V with users. The key is used to encrypt data transmitted to the servers or users. In privacy based authentication protocols besides the user and its home server, no one including the foreign server can ...

  4. OptGS: An R Package for Finding Near-Optimal Group-Sequential Designs

    OpenAIRE

    James Wason

    2015-01-01

    A group-sequential clinical trial design is one in which interim analyses of the data are conducted after groups of patients are recruited. After each interim analysis, the trial may stop early if the evidence so far shows the new treatment is particularly effective or ineffective. Such designs are ethical and cost-effective, and so are of great interest in practice. An optimal group-sequential design is one which controls the type-I error rate and power at a specified level, but minimizes th...

  5. A phylogenomic gene cluster resource: The phylogeneticallyinferred groups (PhlGs) database

    Energy Technology Data Exchange (ETDEWEB)

    Dehal, Paramvir S.; Boore, Jeffrey L.

    2005-08-25

    We present here the PhIGs database, a phylogenomic resource for sequenced genomes. Although many methods exist for clustering gene families, very few attempt to create truly orthologous clusters sharing descent from a single ancestral gene across a range of evolutionary depths. Although these non-phylogenetic gene family clusters have been used broadly for gene annotation, errors are known to be introduced by the artifactual association of slowly evolving paralogs and lack of annotation for those more rapidly evolving. A full phylogenetic framework is necessary for accurate inference of function and for many studies that address pattern and mechanism of the evolution of the genome. The automated generation of evolutionary gene clusters, creation of gene trees, determination of orthology and paralogy relationships, and the correlation of this information with gene annotations, expression information, and genomic context is an important resource to the scientific community.

  6. Phantom bursting is highly sensitive to noise and unlikely to account for slow bursting in beta-cells

    DEFF Research Database (Denmark)

    Pedersen, Morten Gram

    2007-01-01

    cells are subjected to stochastic fluctuations in plasma membrane currents, which are likely to disturb the bursting mechanism and transform medium bursters into spikers or very fast bursters. We present a polynomial, minimal, phantom burster model and show that noise modifies the plateau fraction and......-called compound bursting can be converted to apparent slow bursting by noise, which could explain why compound bursting and mixed Ca oscillations are seen mainly in intact islets....

  7. Carbon steel protection in G.S. (Girlder sulfide) plants. Iron sulfide scales formation conditions. Pt. 1

    International Nuclear Information System (INIS)

    An ASTM A 516 degree 60 carbon steel superficial protection technique submitted to a hydrogen-water sulfide corrosive medium at 2 MPa of pressure and 40-125 deg C forming on itself an iron sulfide layer was tested. Studies on pH influence, temperature, passivating mean characteristics and exposure time as well as the mechanical resistance of sulfide layers to erosion are included. (Author)

  8. Enhanced $0_{g.s.}^+ \\to 2_1^+$ E2 Transition Strength in $^{112,114}$Sn

    CERN Document Server

    Kumar, R; Jhingan, A; Muralithar, S; Mukherjee, S; Reiter, P; Gorska, M; Bhowmik, R K; Cederkall, J; Siwal, D; Doornenbal, P; Garg, R; Appannababu, S; Kojouharov, I; Grawe, H; Singh, P P; Mandal, S; Sharma, A; Schaffner, H; Ekstrom, A; Caceres, L; Prokopowicz, W; Kaur, J; Gerl, J; Singh, R P; Bednarczyk, P

    2011-01-01

    The poorly known B(E2; 0(+) -> 2(+)) values of (112)Sn and (114)Sn have been measured to high precision. Two Coulomb excitation experiments were performed to determine the reduced transition probabilities relative to (116)Sn in order to minimize the systematic errors. The obtained B(E2 up arrow) values of 0.242(8) e(2)b(2) for (112)Sn and 0.232(8) e(2)b(2) for (114)Sn confirm the tendency of large B(E2 up arrow) values for the lighter tin isotopes below the midshell (116)Sn that has been observed recently in various radioactive ion beam experiments.

  9. Procedure for Calculation of Potency and Efficacy for Ligands Acting on G(s)- and G (i)-Coupled Receptors

    DEFF Research Database (Denmark)

    Meier, Eddi; Schousboe, Arne; Belhage, Bo

    2012-01-01

    Structure activity relationship (SAR) analyses of pharmacological data of compounds constitute an important part of the discovery process in the design of new drug candidates with improved pharmacological properties. In particular G-Protein Coupled Receptors (GPCRs) associated with the cAMP secon...... measured signals (cpm or cps) using cAMP standard curves. The present work outlines the mathematical procedures by which such transformations are to be performed....

  10. Lorenzo Scillitani, Antropologia filosofica dei diritti dell'uomo, Bastogi Editrice Italiana, Foggia, 2011, 186 págs.

    OpenAIRE

    Guillermo Hierrezuelo Conde

    2012-01-01

    Scillitani considera la antropología jurídica como la ciencia más adecuada para llevar a cabo un análisis de los derechos del hombre, en la medida en que permite realizar una dimensión inter-cultural (pp. 9-19). La antropología, cultural, social, jurídica nos faculta para conocer el fenómeno jurídico con el fin de poder vislumbrar su identidad. En definitiva, podemos hablar de la prevalencia de la antropología sobre la sociología y la etnología. Pero también ha estudiado la universalidad de l...

  11. Goldsurfer2 (Gs2: A comprehensive tool for the analysis and visualization of genome wide association studies

    Directory of Open Access Journals (Sweden)

    Barnes Michael R

    2008-03-01

    Full Text Available Abstract Background Genome wide association (GWA studies are now being widely undertaken aiming to find the link between genetic variations and common diseases. Ideally, a well-powered GWA study will involve the measurement of hundreds of thousands of single nucleotide polymorphisms (SNPs in thousands of individuals. The sheer volume of data generated by these experiments creates very high analytical demands. There are a number of important steps during the analysis of such data, many of which may present severe bottlenecks. The data need to be imported and reviewed to perform initial quality control (QC before proceeding to association testing. Evaluation of results may involve further statistical analysis, such as permutation testing, or further QC of associated markers, for example, reviewing raw genotyping intensities. Finally significant associations need to be prioritised using functional and biological interpretation methods, browsing available biological annotation, pathway information and patterns of linkage disequilibrium (LD. Results We have developed an interactive and user-friendly graphical application to be used in all steps in GWA projects from initial data QC and analysis to biological evaluation and validation of results. The program is implemented in Java and can be used on all platforms. Conclusion Very large data sets (e.g. 500 k markers and 5000 samples can be quality assessed, rapidly analysed and integrated with genomic sequence information. Candidate SNPs can be selected and functionally evaluated.

  12. De novo sequence assembly and characterization of Lycoris aurea transcriptome using GS FLX titanium platform of 454 pyrosequencing.

    Directory of Open Access Journals (Sweden)

    Ren Wang

    Full Text Available BACKGROUND: Lycoris aurea, also called Golden Magic Lily, is an ornamentally and medicinally important species of the Amaryllidaceae family. To date, the sequencing of its whole genome is unavailable as a non-model organism. Transcriptomic information is also scarce for this species. In this study, we performed de novo transcriptome sequencing to produce the first comprehensive expressed sequence tag (EST dataset for L. aurea using high-throughput sequencing technology. METHODOLOGY AND PRINCIPAL FINDINGS: Total RNA was isolated from leaves with sodium nitroprusside (SNP, salicylic acid (SA, or methyl jasmonate (MeJA treatment, stems, and flowers at the bud, blooming, and wilting stages. Equal quantities of RNA from each tissue and stage were pooled to construct a cDNA library. Using 454 pyrosequencing technology, a total of 937,990 high quality reads (308.63 Mb with an average read length of 329 bp were generated. Clustering and assembly of these reads produced a non-redundant set of 141,111 unique sequences, comprising 24,604 contigs and 116,507 singletons. All of the unique sequences were involved in the biological process, cellular component and molecular function categories by GO analysis. Potential genes and their functions were predicted by KEGG pathway mapping and COG analysis. Based on our sequence analysis and published literatures, many putative genes involved in Amaryllidaceae alkaloids synthesis, including PAL, TYDC OMT, NMT, P450, and other potentially important candidate genes, were identified for the first time in this Lycoris. Furthermore, 6,386 SSRs and 18,107 high-confidence SNPs were identified in this EST dataset. CONCLUSIONS: The transcriptome provides an invaluable new data for a functional genomics resource and future biological research in L. aurea. The molecular markers identified in this study will provide a material basis for future genetic linkage and quantitative trait loci analyses, and will provide useful information for functional genomic research in future.

  13. Monolithisch integrierter 28 GS/s 6 Bit Digital/Analog-Wandler für Echtzeitanwendungen

    OpenAIRE

    Alpert, Thomas

    2013-01-01

    Schnelle D/A-Wandler in CMOS-Technologie sind aufgrund der Integrationsmöglichkeit mit einem DSP auf einem CMOS-Chip sehr attraktiv. Die Systemintegration eröffnet eine Vielzahl von Anwendungsmöglichkeiten, insbesondere in der optischen oder ultrabreitbandingen Telekommunikation. Der in dieser Arbeit veröffentlichte D/A-Wandler wurde im Rahmen des europäischen Projektes „100GET“ für die optische Datenübertragung mit Datenraten bis zu 100 Gbit/s entwickelt. Dabei werden moderne Modulationsform...

  14. Near-complete genome sequencing of swine vesicular disease virus using the Roche GS FLX sequencing platform

    DEFF Research Database (Denmark)

    Nielsen, Sandra Cathrine Abel; Bruhn, Christian Anders Wathne; Samaniego Castruita, Jose Alfredo; Wadsworth, Jemma; Knowles, Nick J.; Gilbert, M Thomas P

    2014-01-01

    Swine vesicular disease virus (SVDV) is an enterovirus that is both genetically and antigenically closely related to human coxsackievirus B5 within the Picornaviridae family. SVDV is the causative agent of a highly contagious (though rarely fatal) vesicular disease in pigs. We report a rapid meth...

  15. Building High School Science Department Inventory Records Using the Appleworks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual was developed for use as a "how to" training device and provides a step-by-step introduction to using AppleWorks in the database mode. Instructions are given to prepare the original database with the headings of the user's choice. Inserting information records in the new database is covered, along with changing the layout of the…

  16. Developing Simple Financial Records Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Duodisk Drive.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing simple financial records using the AppleWorks spreadsheet subprogram with an Apple IIe or Apple IIGS Computer which has a Duodisk or two disk drives. The manual provides step-by-step directions, and includes 34 figures depicting the computer screen at the various stages of the spreadsheet…

  17. Developing Simple Budgets Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Single Disk Drive.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing spreadsheet files in the AppleWorks program using an Apple IIe or Apple IIGS Computer with a single disk drive. The manual provides step-by-step directions, and includes 36 figures depicting the computer screen at the various stages of the spreadsheet development sequence. (EW)

  18. Developing Inventory Records Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing inventory records in the AppleWorks program using an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 17 figures depicting the computer screen at the various stages of the inventory records…

  19. Developing Data Base Files Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    Developed around the technology of the Apple duodisk drive, five and one-fourth inch floppy disks, and the 1.3 version of the AppleWorks program, this manual is designed for use as a "how to" training device in developing database files. The guide is meant to be used with Apple IIe or IIGS computers which have a duodisk or two disk drives and an…

  20. Building Parts Inventory Files Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for building database files using the AppleWorks program with an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 25 figures depicting the computer screen at the various stages of the database file building…

  1. Setting Up Letters Using the AppleWorks Word Processor Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for setting up letter word processing files using the AppleWorks program with an Apple IIe or Apple IIGS computer which has a Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 16 figures depicting the computer screen at the various stages of word…

  2. Altered myocardial Gs protein and adenylyl cyclase signaling in rats exposed to chronic hypoxia and normoxic recovery

    Czech Academy of Sciences Publication Activity Database

    Hrbasová, M.; Novotný, Jiří; Hejnová, Lucie; Kolář, František; Neckář, Jan; Svoboda, Petr

    2003-01-01

    Roč. 94, č. 6 (2003), s. 2423-2432. ISSN 8750-7587 R&D Projects: GA ČR GA305/00/1660; GA ČR GA305/01/0279; GA MŠk LN00A026; GA MŠk LN00A069 Institutional research plan: CEZ:AV0Z5011922 Keywords : rat myocardium * beta-adrenoceptor * G protein Subject RIV: CE - Biochemistry Impact factor: 3.027, year: 2003

  3. NuSTAR detection of 4s Hard X-ray Lags from the Accreting Pulsar GS 0834-430

    DEFF Research Database (Denmark)

    Bachetti, Matteo; Miyasaka, Hiromasa; Harrison, Fiona; Fürst, Felix; Barret, Didier; Bellm, Eric C.; Boggs, Steven E.; Chakrabarty, Deepto; Chenevez, Jérôme; Christensen, Finn Erland; Craig, William W.; Grefenstette, Brian W.; Hailey, Charles J.; Madsen, Kristin K.; Natalucci, Lorenzo; Pottschmidt, Katja; Stern, Daniel; Tomsick, John A.; Walton, Dominic J.; Wilms, Jörn; Zhang, William

    2014-01-01

    consistent with that observed in many other magnetized accreting pulsars. We fail to detect cyclotron resonance scattering features in either phase-averaged nor phase-resolved spectra that would allow us to constrain the pulsar’s magnetic field. We detect a pulse period of ∼ 12.29 s in all energy bands. The...

  4. Nustar Detection of Hard X-Ray Phase Lags from the Accreting Pulsar GS 0834-430

    DEFF Research Database (Denmark)

    Miyasaka, Hiromasa; Bachetti, Matteo; Harrison, Fiona A.; Fu¨rst, Felix; Barret, Didier; Bellm, Eric C.; Boggs, Steven E.; Chakrabarty, Deepto; Chenevez, Jérôme; Christensen, Finn Erland; Craig, William W.; Grefenstette, Brian W.; Hailey, Charles J.; Madsen, Kristin K.; Natalucci, Lorenzo; Pottschmidt, Katja; Stern, Daniel; Tomsick, John A.; Walton, Dominic J.; Wilms, Jo¨rn; Zhang, William

    2013-01-01

    V with high statistical significance. We find the phase-averaged spectrum to be consistent with that observed in many other magnetized, accreting pulsars. We fail to detect cyclotron resonance scattering features that would allow us to constrain the pulsar's magnetic field in either phase-averaged or...

  5. Draft Genome Sequencing of Giardia intestinalis Assemblage B Isolate GS: Is Human Giardiasis Caused by Two Different Species?

    OpenAIRE

    Franzén, Oscar; Jerlström-Hultqvist, Jon; Castro, Elsie; Sherwood, Ellen; Ankarklev, Johan; Reiner, David S.; Palm, Daniel; Andersson, Jan O.; Andersson, Björn; Svärd, Staffan G.

    2009-01-01

    Author Summary Giardia intestinalis is a major contributor to the enormous burden of diarrheal diseases with 250 million symptomatic infections per year, and it is part of the WHO neglected disease initiative. Nonetheless, there is poor insight into how Giardia causes disease; it is not invasive, secretes no known toxin and both the duration and symptoms of giardiasis are highly variable. Currently, there are seven defined variants (assemblages) of G. intestinalis, with only assemblages A and...

  6. Structural flexibility of the G alpha s alpha-helical domain in the beta2-adrenoceptor Gs complex

    DEFF Research Database (Denmark)

    Westfield, Gerwin H; Rasmussen, Søren Gøgsig Faarup; Su, Min; Dutta, Somnath; DeVree, Brian T; Chung, Ka Young; Calinski, Diane; Velez-Ruiz, Gisselle; Oleskie, Austin N; Pardon, Els; Chae, Pil Seok; Liu, Tong; Li, Sheng; Woods, Virgil L; Steyaert, Jan; Kobilka, Brian K; Sunahara, Roger K; Skiniotis, Georgios

    2011-01-01

    The active-state complex between an agonist-bound receptor and a guanine nucleotide-free G protein represents the fundamental signaling assembly for the majority of hormone and neurotransmitter signaling. We applied single-particle electron microscopy (EM) analysis to examine the architecture of ...

  7. Report on the use of programmable digital computers in the shutdown systems of the Darlington G.S

    International Nuclear Information System (INIS)

    This report by the Advisory Committee on Nuclear Safety (ACNS) considers the use of large, programmable computers in the shutdown system at the planned Darlington Generating Station. After a review of the documents submitted to the Atomic Energy Control Board (AECB) by Atomic Energy of Canada Ltd. Engineering Company in support of gaining AECB's agreement in principle to such a system, the ACNS concludes that no fundamental principle will be challenged by the introduction of computer technology into safety systems. It cautions, however, that the AECB should ensure that existing principles, particularly those of independence and redundancy, will not be compromised in the application

  8. The QUAX-g_p g_s experiment to search for monopole-dipole Axion interaction

    CERN Document Server

    Crescini, Nicolò; Carugno, Giovanni; Falferi, Paolo; Ortolan, Antonello; Ruoso, Giuseppe

    2016-01-01

    The QCD axion is an hypothetical particle introduced to solve the strong CP problem of standard model of particle physics and is of interest as a possible component of cold dark matter. In the axion scenario, J.E. Moody and F. Wilczek showed that a new macroscopic long-range force, mediated by axion exchange, acts on electron spins, and that such force can be described in terms of an effective magnetic field. The QUAX-g$_p$g$_s$ experiment, carried out at INFN Laboratori Nazionali di Legnaro, is designed to search for the effects on magnetized samples of the effective field produced by unpolarized mass sources. As this field is macroscopic, it can be detected by measuring the change of magnetization of a paramagnetic Gadolinium silicate (GSO) crystal cooled at liquid helium temperature. The axion effective field induced magnetization can be detected with a SQUID magnetometer. By varying the position of the of source masses, the induced GSO magnetization is modulated at acoustic frequencies. Although the full ...

  9. GS-8374, a Prototype Phosphonate-Containing Inhibitor of HIV-1 Protease, Effectively Inhibits Protease Mutants with Amino Acid Insertions

    Czech Academy of Sciences Publication Activity Database

    Grantz Šašková, Klára; Kožíšek, Milan; Stray, K.; Jong de, D.; Řezáčová, Pavlína; Brynda, Jiří; Maarseveen van, N. M.; Nijhuis, M.; Cihlář, T.; Konvalinka, Jan

    2014-01-01

    Roč. 88, č. 6 (2014), s. 3586-3590. ISSN 0022-538X R&D Projects: GA ČR GAP207/11/1798 Grant ostatní: OPPC(XE) CZ.2.16/3.1.00/24016 Institutional support: RVO:61388963 ; RVO:68378050 Keywords : virus type-1 protease * antiviral activity * drug resistance Subject RIV: EE - Microbiology, Virology Impact factor: 4.439, year: 2014

  10. Metal properties of centrifugal cast pipes of 15Kh1M1F and 15 GS steels

    International Nuclear Information System (INIS)

    In the present work the properties of the parent metla of experimental-industrial batches of centrifugally-cast tubes of 15Cr1Mo1V and 15MnSi (16MnSi) steel are investigated. The specimens were tested for creep and rupture strength; the macro- and microstructures of a large number of sections were studied. The tubes were carefully tested ultrasonically with the aid of a UDM1F flaw detector. A characteristic of the tube metal was the fairly dense structure. The results obtained showed that it is possible to use centrifugally-cast tubes, but that it is necessary to carry out improvement work and to ensure stability of the cycle of fabrication and treatment of the tubes

  11. Carbon steel protection in G.S. (Girlder sulfide) plants. Influence of the material surface state. Pt. 2

    International Nuclear Information System (INIS)

    The passivation on carbon steels, in particular ASTM A 516 Degree 60 and ASTM A 333 steels is made, submitting it to the action of H2S/H2O1,2 corrosive medium. The steel is rapidly corroded by H2S in aqueous solution, forming iron sulfides on the metallic surface in a crystalline layer of various μm of thickness. During this process, various types of iron sulfides at different phases, with different sulfur and iron contents are formed. The influence of temperature, the pH, the exposure time and the corrosive medium composition on formation and quality of the iron sulfides protective layer was also studied. (Author)

  12. Inspection of radiation sources and regulatory enforcement (supplement to IAEA Safety Standards Series No. GS-G-1.5)

    International Nuclear Information System (INIS)

    The achievement and maintenance of a high level of safety in the use of radiation sources depends on there being a sound legal and governmental infrastructure, including a national regulatory body with well-defined responsibilities and functions. These responsibilities and functions include establishing and implementing a system for carrying out regulatory inspections, and taking necessary enforcement actions. The Safety Requirements publication entitled Legal and Governmental Infrastructure for Nuclear, Radiation, Radioactive Waste and Transport Safety establishes the requirements for legal and governmental infrastructure. The term 'infrastructure' refers to the underlying structure of systems and organizations. This includes requirements concerning the establishment of a regulatory body for radiation sources and the responsibilities and functions assigned to it. The International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources (the Basic Safety Standards or the BSS) establish basic requirements for protection against risks associated with exposure to ionizing radiation and for the safety of radiation sources. The application of the BSS is based on the presumption that national infrastructures are in place to enable governments to discharge their responsibilities to for radiation protection and safety. This TECDOC provides practical guidance on the processes for carrying out regulatory inspections and taking enforcement actions. It includes information on the development and use of procedures and standard review plans (i.e. checklists) for inspection. Specific procedures for inspection of radiation practices and sources are provided in the Appendices

  13. Phantom bursting is highly sensitive to noise and unlikely to account for slow bursting in beta-cells

    DEFF Research Database (Denmark)

    Pedersen, Morten Gram

    2007-01-01

    Pancreatic beta-cells show bursting electrical activity with a wide range of burst periods ranging from a few seconds, often seen in isolated cells, over tens of seconds (medium bursting), usually observed in intact islets, to several minutes. The phantom burster model [Bertram, R., Previte, J...... cells are subjected to stochastic fluctuations in plasma membrane currents, which are likely to disturb the bursting mechanism and transform medium bursters into spikers or very fast bursters. We present a polynomial, minimal, phantom burster model and show that noise modifies the plateau fraction and...... lowers the burst period dramatically in phantom bursters. It is therefore unlikely that slow bursting in single cells is driven by the slow phantom bursting mechanism, but could instead be driven by oscillations in glycolysis, which we show are stable to random ion channel fluctuations. Moreover, so...

  14. Generalized Polarization Effect and B(E2,2+1→0+g.s.) in Neutron Rich Even Carbon Isotopes

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>The generalized polarization effects on electric quadrupole transitions is proposed and studied by the microscopic particle-vibration model. The static polarization effects on neutron and proton vertex are

  15. Developing International Research Collaborations among Postdoctoral Fellows: Key Findings from the Evaluation of NSF's International Research Fellowship Program. GS-10F-0086K

    Science.gov (United States)

    Martinez, Alina; Epstein, Carter; Parsad, Amanda; Whittaker, Karla

    2012-01-01

    Over a decade ago, the National Science Board (NSB) highlighted the importance of international collaboration in its call for increased government commitment to promoting international science and engineering (S&E) research and education. The NSB also identified the National Science Foundation (NSF) as having an important leadership role in…

  16. Bourke, Joanna. Los violadores: historia del estupro de 1860 a nuestros días. Barcelona: Crítica, 2009, 702 págs

    OpenAIRE

    Ingrid Viviana Serrano Ramírez

    2012-01-01

    Joanna Bourke es nacida en Blenheim, Nueva Zelanda en el año de 1963, actualmente es catedrática de Historia en el Birkbeck College, de la Universidad de Londres. se describe a sí misma como una «socialista feminista», ha tratado un amplio abanico de temas en sus investigaciones: la historia de la clase obrera y el trabajo de las mujeres, las experiencias de los hombres y las mujeres durante la guerra, la historia de las emociones, especialmente del miedo y el odio, y la historia de la violen...

  17. Bourke, Joanna. Los violadores: historia del estupro de 1860 a nuestros días. Barcelona: Crítica, 2009, 702 págs

    Directory of Open Access Journals (Sweden)

    Ingrid Viviana Serrano Ramírez

    2012-07-01

    Full Text Available Joanna Bourke es nacida en Blenheim, Nueva Zelanda en el año de 1963, actualmente es catedrática de Historia en el Birkbeck College, de la Universidad de Londres. se describe a sí misma como una «socialista feminista», ha tratado un amplio abanico de temas en sus investigaciones: la historia de la clase obrera y el trabajo de las mujeres, las experiencias de los hombres y las mujeres durante la guerra, la historia de las emociones, especialmente del miedo y el odio, y la historia de la violencia sexual. Actualmente, dirige una investigación sobre el dolor corporal a lo largo de los dos últimos siglos. Entre sus libros están traducidos La Segunda Guerra Mundial: una historia de las víctimas Empúries, 2003; Paidós, 2002, Sed de sangre: historia íntima del combate cuerpo a cuerpo en las guerras del siglo XX (Crítica, 2008 y Los violadores: historia del estupro de 1860 a nuestros días (Crítica, 2009. Recientemente, ha publicado What it Means to be Human. Reflections from 1791 to the Present (Virago, 2011.

  18. William F. Sater, La imagen heroica de Chile: Arturo Prat, santo secular, Santiago, Centro de Estudios Bicentenario, 2005, 231 págs.

    Directory of Open Access Journals (Sweden)

    SANTIAGO ARÁNGUIZ PINTO

    2011-10-01

    Full Text Available LA PERSONA Y LA FIGURA de Arturo Prat parece que, a simple vista, representan una misma y única unidad. Pero, si profundizamos algo más en la vida de este marino y abogado, nos daremos cuenta de que sus datos biográficos y la percepción que tuvo la sociedad de éste, varían considerablemente, pues, por su misma condición de héroe, está sujeto a los vaivenes interpretativos propios de la sobreabundante mitología nacional. Y es que el héroe, dice Sater, plantea otras problemáticas y disyuntivas cuando se le estudia, diferente a las del hombre público que posee figuración o renombre. En el héroe, por el contrario, confluyen elementos que responden a otro tipo de variables sociales, espirituales y culturales, y que es, por cierto, la preocupación fundamental que se ha propuesto desarrollar en este libro el historiador norteamericano. Analizar, comprender o estudiar la imagen heroica de Prat exige la participación de otros elementos analíticos que, a su vez, se plantean en relación a determinados momentos de la historia de Chile. No hay dudas al respecto: a nadie dejaba indiferente los rasgos y virtudes que desprendía la cautivante personalidad de Prat, considerando que éste era un ejemplo único de abnegación, heroísmo y de entrega hacia su patria, digno de imitar y difundir hacia el resto de la sociedad. Cada vez que ocurría alguna desgracia, el país requería a su vez de un estímulo moral para superar los problemas nacionales. ¿Cómo lograrlo? Recurriendo a Prat, que luego se transformaría en una práctica común, que excedió los límites de la mesura y el entendimiento.

  19. Gérard Guyon, La Règle de Saint Benoît: aux sources du droit..., DMM, Poitiers Cedex, 2012, 190 págs.

    Directory of Open Access Journals (Sweden)

    Guillermo Hierrezuelo Conde

    2013-03-01

    Full Text Available En esta monografía Guyon estudia ampliamente la Regla de San Benito, fundada por su homónimo, considerado como uno de los "Padres de Europa". Sus reglas, influidas en gran medida por el Derecho Romano, alcanzaron gran predicamento sobre todo a partir del siglo VI. Los dos pilares básicos que conformaban la Regla benedictina eran el oficio divino, de una parte, y la lección divina o el trabajo, de otra. San Benito obligaba a dedicar entre cuatro y ocho horas diarias al oficio divino y siete horas para dormir; y las restantes horas a trabajar, a estudiar o a la lectura religiosa. En caso de incumplimiento de las normas se establecían sanciones, que tenían su fundamento en el Derecho privado romano. Asimismo, diferenciaba entre la culpa y el dolo. Además, la Regla se basaba en los principios de la caridad y el acogimiento, si bien limitaba al máximo la permanencia de extranjeros dentro de la comunidad, para evitar que surgieran conflictos internos. La figura del abad en los monasterios adquiría un papel relevante en la medida en que presentaba como un auténtico Padre, pero como un Padre que juzgaba.

  20. High-temperature cast steel GS-17 CrMoV 5 11 - a contribution to the behaviour of simulated HAZ structures

    International Nuclear Information System (INIS)

    Different heat-affected-zone structures of two creep-resistant cast-steel materials containing 1% CrMoV (spec. according to DIN 17245 or ASTM A 356) are simulated by using the welding simulator GLEEBLE 1500. The conversing attitude in the heat-affected zone was investigated and illustrated by means of a welding time temperature transition curve. The mechanical behaviour of the heat-affected-zone structures was determined by means of thermal tension tests at 5500C and thoughness tests. The material according to the ASTM specifications shows a better toughness behaviour. The influence of different thermal treatments on the toughness behaviour of the coarse grain zone was investigated. Best values of toughness were achieved at annealing temperatures of 700 and 7250C. Tests on stress relief cracking revealed that both materials are susceptible to cracking in the temperature range from 550 to 6500C. The precipitation condition of the coarse grain zone after various thermal treatments was investigated by using the electron microscope. Fine MX and M2C precipitations of the mixed carbides of the type M23C6 M7C3 were detected in dependence on the annealing temperature. (orig./MM)

  1. Carbon steel protection in G.S. (Girlder sulfide) plants. Iron sulfide scales formation on surfaces covered by fabrication produced films. Pt. 4

    International Nuclear Information System (INIS)

    This work describes the assays aimed to passivate the steel carbon of the process pipings. This steel is marked by the ASTM A 333 G6 and is chemically similar to those of isotopic exchange towers which corrode in contact with in-water hydrogen sulfide solutions forming iron sulfide protective layers. The differences between both materials lie in the surface characteristics to be passivated. The steel of towers has an internal side covered by paint which shall be removed prior to passivation. The steel's internal side shall be covered by a film formed during the fabrication process and constituted by calcinated wastes and iron oxides (magnetite, hematite and wustite). This film interferes in the formation process of passivating layers of pyrrhotite and pyrite. The possibility to passivate the pipes in their actual state was evaluated since it would result highly laborious and expensive to eliminate the film. (Author)

  2. Design and Implementation of Automatic Street Light Control Using Sensors and Solar Panel Sharath Patil G.S1 , Rudresh S.M

    Directory of Open Access Journals (Sweden)

    Sharath Patil G.S

    2015-06-01

    Full Text Available Solar Photovoltaic panel based street lighting systems are becoming more common these days. But the limitation with these ordinary street light systems is that it lacks intelligent performance. It is very essential to automate the system so that we can conserve energy as well as to maximize the efficiency of the system. In this paper a new method is suggested so as to maximize the efficiency of the street lighting system and to conserve the energy usage the LED lights sensors. Here automation of street lights is done by LDR sensor. Intensity of led street lights can be controlled by IR sensor and pulse width modulation. Kewords:

  3. Cosmarium pokornyanum (Grunow) W. et G.S. West, een arctisch-alpiene desmidiaceeënsoort nieuw voor de Nederlandse flora

    OpenAIRE

    Coesel, Peter F.M.; Meesters, Koos (J.)

    2001-01-01

    Cosmarium pokornyanum is principally known from arctic and alpine regions in Europe and North America. In June, 2001, it was found for the first time in the Netherlands. Its occurrence in this temperate lowland region may be associated with its ecology, i.e., a sub-atmophytic way of life on pH-neutral to alkaline substrates.

  4. Assimilation of Soil Wetness Index and Leaf Area Index into the ISBA-A-gs land surface model: grassland case study

    OpenAIRE

    Barbu, A. L.; J.-C. Calvet; J.-F. Mahfouf; Albergel, C.; Lafont, S.

    2011-01-01

    The performance of the joint assimilation in a land surface model of a Soil Wetness Index (SWI) product provided by an exponential filter together with Leaf Area Index (LAI) is investigated. The data assimilation is evaluated with different setups using the SURFEX modeling platform, for a period of seven years (2001–2007), at the SMOSREX grassland site in southwestern France. The results obtained with a Simplified Extended Kalman Filter demonstrate the effectiveness of a joint data assimilati...

  5. Negative Unintended Consequences of Innovation- a case study regarding innovation and sustainability : The new Extended Value Creation Mechanism for Global Sustainability, the SNE SFI GS-framework

    OpenAIRE

    Ali Rashed, Chra; Mouyiasis, George

    2013-01-01

    Innovation is often related to positive outcomes for the majority to enjoy that enhances welfare and facilitates everyday activities. In different fields of study (economics, management, sociology, history and science and technology) it is becoming a field of magnitude (Malerba and Brusoni, 2007). Fagerberg and Verspagen (2009) discuss the emerging interest of innovation in the field of social science. Although the interest within the subject of innovation is high, very little research has be...

  6. Detection of IgM antibrucella antibody in the absence of IgGs: a challenge for the clinical interpretation of brucella serology.

    OpenAIRE

    Julián Solís García Del Pozo; Santiago Lorente Ortuño; Elena Navarro; Javier Solera

    2014-01-01

    The use of enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM antibodies antibrucella has become widespread in the diagnosis of human brucellosis. IgM anti-Brucella antibodies are indicative of acute infection. Between 2009-2013, 5307 patients were evaluated for serologic diagnosis at the Microbiology Laboratory of the Albacete General Hospital. A ELISA IgM-positive, IgG-negative anti-Brucella antibody serology pattern was detected in 17 of those patients. Epidemiology...

  7. Detection of IgM antibrucella antibody in the absence of IgGs: a challenge for the clinical interpretation of brucella serology.

    Directory of Open Access Journals (Sweden)

    Julián Solís García Del Pozo

    2014-12-01

    Full Text Available The use of enzyme-linked immunosorbent assay (ELISA for the detection of IgG and IgM antibodies antibrucella has become widespread in the diagnosis of human brucellosis. IgM anti-Brucella antibodies are indicative of acute infection. Between 2009-2013, 5307 patients were evaluated for serologic diagnosis at the Microbiology Laboratory of the Albacete General Hospital. A ELISA IgM-positive, IgG-negative anti-Brucella antibody serology pattern was detected in 17 of those patients. Epidemiology data, symptoms, laboratory data, treatment and outcome from these patients were reviewed. Sixteen patients presented with musculoskeletal pain, fatigue and/or fever and 1 was asymptomatic. Five patients received treatment with doxycycline combined with rifampin, gentamycin or streptomycin during 6-12 weeks, with no improvement. None of the 17 patients were finally diagnosed with brucellosis. Our results indicate that anti-Brucella IgM positive serology, per se, is not enough to diagnose acute brucellosis and other methods should be used for confirmation. Brucella serology data should be interpreted taking into account the patient's clinical history and epidemiological context.

  8. Modified paired end rapid library preparation protocol for 454 GS Junior 8 kb library preparation using Covaris g-tubes and BluePippin electrophoresis

    OpenAIRE

    sprotocols

    2014-01-01

    Authors: Janet Hill, Bonnie Chaban, Jennifer Town, Matthew Links & Tim Dumonceaux ### Abstract This protocol describes an alternative approach to performing Roche’s Paired End Rapid Library Preparation Method for 8 kb span libraries. This method uses the Corvaris g-tube for DNA fragmentation, eliminating the need for a HydroShear apparatus, and a Sage Science BluePippin electrophoresis platform to size select the 8 kb fragments, eliminating the need for agarose gel electrophoresi...

  9. Comparing the accuracy of ES-BC, EIS-GS, and ES Oxi on body composition, autonomic nervous system activity, and cardiac output to standardized assessments

    OpenAIRE

    Lewis JE; Tannenbaum SL; Gao J; Melillo AB; Long EG; Alonso Y; Konefal J; Woolger JM; Leonard S; Singh PK; Chen L; Tiozzo E

    2011-01-01

    John E Lewis1, Stacey L Tannenbaum1, Jinrun Gao3, Angelica B Melillo1, Evan G Long1, Yaima Alonso2, Janet Konefal1, Judi M Woolger2, Susanna Leonard1, Prabjot K Singh1, Lawrence Chen1, Eduard Tiozzo1 1Department of Psychiatry and Behavioral Sciences, 2Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, 3State Farm Insurance, Bloomington, IL, USA Background and purpose: The Electro Sensor Complex (ESC) is software that combines three devices using bioelectrical i...

  10. De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis

    Directory of Open Access Journals (Sweden)

    Lui Edmund MK

    2010-04-01

    Full Text Available Abstract Background American ginseng (Panax quinquefolius L. is one of the most widely used herbal remedies in the world. Its major bioactive constituents are the triterpene saponins known as ginsenosides. However, little is known about ginsenoside biosynthesis in American ginseng, especially the late steps of the pathway. Results In this study, a one-quarter 454 sequencing run produced 209,747 high-quality reads with an average sequence length of 427 bases. De novo assembly generated 31,088 unique sequences containing 16,592 contigs and 14,496 singletons. About 93.1% of the high-quality reads were assembled into contigs with an average 8-fold coverage. A total of 21,684 (69.8% unique sequences were annotated by a BLAST similarity search against four public sequence databases, and 4,097 of the unique sequences were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes. Based on the bioinformatic analysis described above, we found all of the known enzymes involved in ginsenoside backbone synthesis, starting from acetyl-CoA via the isoprenoid pathway. Additionally, a total of 150 cytochrome P450 (CYP450 and 235 glycosyltransferase unique sequences were found in the 454 cDNA library, some of which encode enzymes responsible for the conversion of the ginsenoside backbone into the various ginsenosides. Finally, one CYP450 and four UDP-glycosyltransferases were selected as the candidates most likely to be involved in ginsenoside biosynthesis through a methyl jasmonate (MeJA inducibility experiment and tissue-specific expression pattern analysis based on a real-time PCR assay. Conclusions We demonstrated, with the assistance of the MeJA inducibility experiment and tissue-specific expression pattern analysis, that transcriptome analysis based on 454 pyrosequencing is a powerful tool for determining the genes encoding enzymes responsible for the biosynthesis of secondary metabolites in non-model plants. Additionally, the expressed sequence tags (ESTs and unique sequences from this study provide an important resource for the scientific community that is interested in the molecular genetics and functional genomics of American ginseng.

  11. Biased Gs Versus Gq Proteins and β-Arrestin Signaling in the NK1 Receptor Determined by Interactions in the Water Hydrogen Bond Network

    DEFF Research Database (Denmark)

    Valentin-Hansen, Louise; Frimurer, Thomas M; Mokrosinski, Jacek; Holliday, Nicholas D; Schwartz, Thue W

    2015-01-01

    X-ray structures, molecular dynamics simulations, and mutational analysis have previously indicated that an extended water hydrogen bond network between trans-membranes I-III, VI, and VII constitutes an allosteric interface essential for stabilizing different active and inactive helical...... the highly conserved AspII:10 (2.50). Here, we find that this GluII:10 occupies the space of a putative allosteric modulating Na(+) ion and makes direct inter-helical interactions in particular with SerIII:15 (3.39) and AsnVII:16 (7.49) of the NPXXY motif. Mutational changes in the interface between...

  12. Design and Implementation of Automatic Street Light Control Using Sensors and Solar Panel Sharath Patil G.S1 , Rudresh S.M

    OpenAIRE

    Sharath Patil G.S; Rudresh S.M

    2015-01-01

    Solar Photovoltaic panel based street lighting systems are becoming more common these days. But the limitation with these ordinary street light systems is that it lacks intelligent performance. It is very essential to automate the system so that we can conserve energy as well as to maximize the efficiency of the system. In this paper a new method is suggested so as to maximize the efficiency of the street lighting system and to conserve the energy usage the LED lights sensors. Her...

  13. Blank Computer Floppy Disk Formatting Using the AppleWorks Program, Apple IIe or GS Computers and a Duodisk or Two Disk Drives.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for formatting blank floppy disks in the AppleWorks program using an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives. The manual provides step-by-step directions, and includes 11 figures depicting the computer screen at the various stages of the formatting sequence. (EW)

  14. Developing Simple Grade Books Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Duodisk or Two Disk Drives.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing grade books using the AppleWorks spreadsheet subprogram with an Apple IIe or Apple IIGS Computer which has a Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 41 figures depicting the computer screen at the various stages of the…

  15. Antonio María Baggio, Adriana Cosseddu y Antonio Márquez Prieto (Coord., Fraternidad y Justicia, Comares, Granada, 2012, 94 págs.

    Directory of Open Access Journals (Sweden)

    Guillermo Hierrezuelo Conde

    2013-03-01

    Full Text Available Esta obra nos ofrece una visión triádica entre libertad, justicia y fraternidad, destacando el carácter novedoso de esta última. En efecto, únicamente un análisis conjunto de las tres categorías permitiría alcanzar, según sus autores, un significado pleno de la fraternidad. Esta relación queda en evidencia en el art. 1 de la Declaración Universal de los Derechos del hombre. De hecho, se destaca a la fraternidad como el principio olvidado de la revolución francesa (liberté, egalité, fraternité. Diversos autores la analizan como categoría política, lo que permitiría corregir algunos errores e injusticias de los sistemas políticos. Por otro lado, en la fraternidad podemos observar la relacionalidad, entendida como las relaciones entre los hombres. Asimismo, cuando hablamos de justicia se requieren tres elementos: la relacionalidad, la reciprocidad y la socialidad, en la medida en que la relación requiere lugar en el espacio social, donde adquiere sentido.

  16. Spearfishing Regulation Benefits Artisanal Fisheries: The ReGS Indicator and Its Application to a Multiple-Use Mediterranean Marine Protected Area

    OpenAIRE

    Delphine Rocklin; Jean-Antoine Tomasini; Jean-Michel Culioli; Dominique Pelletier; David Mouillot

    2011-01-01

    The development of fishing efficiency coupled with an increase of fishing effort led to the overexploitation of numerous natural marine resources. In addition to this commercial pressure, the impact of recreational activities on fish assemblages remains barely known. Here we examined the impact of spearfishing limitation on resources in a marine protected area (MPA) and the benefit it provides for the local artisanal fishery through the use of a novel indicator. We analysed trends in the fish...

  17. Notification and authorization for the use of radiation sources (supplement to IAEA Safety Standards Series No. GS-G-1.5)

    International Nuclear Information System (INIS)

    The achievement and maintenance of a high level of safety in the use of radiation sources depend on there being a sound legal and governmental infrastructure, including a national regulatory body with well-defined responsibilities and functions. These responsibilities and functions include establishing and implementing a system for notification and authorization for control over radiation sources, including a system for review and assessment of applications for authorization. The Safety Requirements publication entitled Legal and Governmental Infrastructure for Nuclear, Radiation, Radioactive Waste and Transport Safety establishes the requirements for legal and governmental infrastructure. The term 'infrastructure' refers to the underlying structure of systems and organizations. This includes requirements concerning the establishment of a regulatory body for radiation sources and the responsibilities and functions assigned to it. The International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources (the Basic Safety Standards or the BSS) establish basic requirements for protection against risks associated with exposure to ionizing radiation and for the safety of radiation sources. The application of the BSS is based on the presumption that national infrastructures are in place to enable governments to discharge their responsibilities to for radiation protection and safety. This TECDOC provides practical guidance on the process for dealing with applications for authorization and accepting notifications to regulatory bodies. Examples of guidelines that may be used by persons required to notify or apply for authorization and of the regulatory body's review and assessment procedures are provided in the Appendices. The TECDOC is oriented towards national regulatory infrastructures concerned with protection and safety for radiation sources used in medicine, industry, agriculture, research and education

  18. GS固砂剂在青海尕斯油田的应用%Application of GS Sand Consolidation Agent in Gasi Qilfield

    Institute of Scientific and Technical Information of China (English)

    张小平; 佘跃惠; 余维初; 胡三清; 罗觉生

    2001-01-01

    针对青海尕斯油田N1一N21油藏出砂严重这一问题,研制出与之相匹配的GS固砂剂,并对在该区域采用直接注固砂剂的固砂工艺进行了室内模拟试验,初步探讨出GS中的固化剂加量、挤入GS量及扩孔液、扩孔体积等对固结强度和渗透率的影响,并优选出合适的固砂剂种类和相关工艺参数,在该工艺条件下,其固结体抗压强度大于10 MPa,渗透率损害小于30%,这些条件对现场施工产生了有效的指导作用.

  19. A characterization of the K-functional for the algebraic version of the trigonometric Jackson integrals Gs,n in weighted integral metric

    Science.gov (United States)

    Zapryanova, Teodora

    2013-12-01

    We introduce moduli of smoothness which are computable characteristic equivalents of the error in approximation by the algebraic versions of trigonometric Jackson integrals in weighted integral metric.

  20. GARCÍA CARRASCO, J. (2007) "Leer en la cara y en el mundo", Barcelona, Herder, 462 págs.

    OpenAIRE

    Fernando BÁRCENA ORBE

    2008-01-01

    "Leer en la cara y en el mundo" es un libro de lecturas y de estudio, de conversaciones prolongadas y de serena meditación, un libro de pedagogía, de antropología y de teoría de la educación que no elude la referencia literario o filosófica. Un libro escrito no porque ya se sepa, sino porque "se quiere conocer mejor", porque se aspira a "comprender" lo que se creía ya sabido, porque busca que el pensar ayude a seguir pensando, y a pensar probablemente de otro modo.

  1. Design challenges of EO polymer based leaky waveguide deflector for 40 Gs/s all-optical analog-to-digital converters

    Science.gov (United States)

    Hadjloum, Massinissa; El Gibari, Mohammed; Li, Hongwu; Daryoush, Afshin S.

    2016-08-01

    Design challenges and performance optimization of an all-optical analog-to-digital converter (AOADC) is presented here. The paper addresses both microwave and optical design of a leaky waveguide optical deflector using electro-optic (E-O) polymer. The optical deflector converts magnitude variation of the applied RF voltage into variation of deflection angle out of a leaky waveguide optical beam using the linear E-O effect (Pockels effect) as part of the E-O polymer based optical waveguide. This variation of deflection angle as result of the applied RF signal is then quantized using optical windows followed by an array of high-speed photodetectors. We optimized the leakage coefficient of the leaky waveguide and its physical length to achieve the best trade-off between bandwidth and the deflected optical beam resolution, by improving the phase velocity matching between lightwave and microwave on one hand and using pre-emphasis technique to compensate for the RF signal attenuation on the other hand. In addition, for ease of access from both optical and RF perspective, a via-hole less broad bandwidth transition is designed between coplanar pads and coupled microstrip (CPW-CMS) driving electrodes. With the best reported E-O coefficient of 350 pm/V, the designed E-O deflector should allow an AOADC operating over 44 giga-samples-per-seconds with an estimated effective resolution of 6.5 bits on RF signals with Nyquist bandwidth of 22 GHz. The overall DC power consumption of all components used in this AOADC is of order of 4 W and is dominated by power consumption in the power amplifier to generate a 20 V RF voltage in 50 Ohm system. A higher sampling rate can be achieved at similar bits of resolution by interleaving a number of this elementary AOADC at the expense of a higher power consumption.

  2. Assimilation of Soil Wetness Index and Leaf Area Index into the ISBA-A-gs land surface model: grassland case study

    OpenAIRE

    A. L. Barbu; Calvet, J.-C.; Mahfouf, J.-F.; Albergel, C.; LAFONT,S

    2011-01-01

    The performance of the joint assimilation in a land surface model of a Soil Wetness Index (SWI) product provided by an exponential filter together with Leaf Area Index (LAI) is investigated. The data assimilation is evaluated with different setups using the SURFEX modeling platform, for a period of seven years (2001–2007), at the SMOSREX grassland site in southwestern France. The results obtained with a Simplified Extended Kalman Filter demonstrate the effectiveness of a joi...

  3. Results and interpretation of groundwater data obtained from multiport-instrumented coreholes (GW-131 through GS-135), fiscal years 1990 and 1991

    International Nuclear Information System (INIS)

    With the increased emphasis by Department of Energy personnel on assessing the environmental impact of past waste disposal practices at all of its facilities, there has been an associated increase in characterization activities that focus on delineating site-specific groundwater flow regimes and contaminant migration pathways. At the Oak Ridge Y-12 Plant, the complex geologic and hydrologic relationships require a more detailed understanding of the three-dimensional properties of groundwater flow regimes than can be obtained by conventional monitoring activities. Thus, as part of groundwater characterization activities conducted by the Environmental Surveillance Section staff of the Y-12 Plant Environmental Management Department, five existing deep core holes were instrumented with multiport monitoring systems to provide greatly enhanced resolution of the hydraulic and hydrochemical properties of the groundwater system within Bear Creek Valley. With a multiport system, it is possible to measure hydraulic head and hydraulic conductivity and collect water samples from multiple levels within a single borehole. In this report, multiport data collected during fiscal years (FYs) 1990 and 1991 are summarized

  4. Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes

    OpenAIRE

    Ma Meng; Yan Yan; Huang Li; Chen Mingshun; Zhao Huixian

    2012-01-01

    Abstract Background The Barley stripe mosaic virus (BSMV)-based vector has been developed and used for gene silencing in barley and wheat seedlings to assess gene functions in pathogen- or insect-resistance, but conditions for gene silencing in spikes and grains have not been evaluated. In this study, we explored the feasibility of using BSMV for gene silencing in wheat spikes or grains. Results Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after13...

  5. Bursting oscillations, bifurcation and synchronization in neuronal systems

    International Nuclear Information System (INIS)

    Highlights: → We investigate bursting oscillations and related bifurcation in the modified Morris-Lecar neuron. → Two types of fast-slow bursters are analyzed in detail. → We show the properties of some crucial bifurcation points. → Synchronization transition and the neural excitability are explored in the coupled bursters. - Abstract: This paper investigates bursting oscillations and related bifurcation in the modified Morris-Lecar neuron. It is shown that for some appropriate parameters, the modified Morris-Lecar neuron can exhibit two types of fast-slow bursters, that is 'circle/fold cycle' bursting and 'subHopf/homoclinic' bursting with class 1 and class 2 neural excitability, which have different neuro-computational properties. By means of the analysis of fast-slow dynamics and phase plane, we explore bifurcation mechanisms associated with the two types of bursters. Furthermore, the properties of some crucial bifurcation points, which can determine the type of the burster, are studied by the stability and bifurcation theory. In addition, we investigate the influence of the coupling strength on synchronization transition and the neural excitability in two electrically coupled bursters with the same bursting type. More interestingly, the multi-time-scale synchronization transition phenomenon is found as the coupling strength varies.

  6. Fracture of explosively compacted aluminum particles in a cylinder

    Science.gov (United States)

    Frost, David; Loiseau, Jason; Goroshin, Sam; Zhang, Fan; Milne, Alec; Longbottom, Aaron

    2015-06-01

    The explosive compaction, fracture and dispersal of aluminum particles contained within a cylinder have been investigated experimentally and computationally. The aluminum particles were weakly confined in a cardboard tube and surrounded a central cylindrical burster charge. The compaction and fracture of the particles are visualized with flash radiography and the subsequent fragment dispersal with high-speed photography. The aluminum fragments produced are much larger than the original aluminum particles and similar in shape to those generated from the explosive fracture of a solid aluminum cylinder, suggesting that the shock transmitted into the aluminum compacts the powder to near solid density. The casing of the burster explosive (plastic-, copper-, and un-cased charges were used) had little influence on the fragment size. The effect of an air gap between the burster and the aluminum particles was also investigated. The particle motion inferred from the radiographs is compared with the predictions of a multimaterial hydrocode.

  7. A UHPLC-MS/MS method for the quantification of direct antiviral agents simeprevir, daclatasvir, ledipasvir, sofosbuvir/GS-331007, dasabuvir, ombitasvir and paritaprevir, together with ritonavir, in human plasma.

    Science.gov (United States)

    Ariaudo, Alessandra; Favata, Fabio; De Nicolò, Amedeo; Simiele, Marco; Paglietti, Luca; Boglione, Lucio; Cardellino, Chiara Simona; Carcieri, Chiara; Di Perri, Giovanni; D'Avolio, Antonio

    2016-06-01

    To date, the new standard for treatment of chronic hepatitis C is based on the administration of novel direct acting antivirals. Among these, sofosbuvir, simeprevir, daclatasvir, ledipasvir, dasabuvir, ombitasvir and paritaprevir already entered the clinical use. Anyway, since few pharmacokinetic studies have been conducted on these drugs in a "real life" context poor knowledge is available about their optimal therapeutic range. Without this background, therapeutic drug monitoring is not applicable for treatment optimization. Up to now, a few methods are reported to quantify these drugs in human plasma, and none of them in a simultaneous way. The aim of this work was to develop and validate a simple, fast and cheap, but still reliable UHPLC-MS/MS method for the quantification of these drugs, feasible for a clinical routine use. Solid phase extraction was performed using HLB C18 96-well plates. Chromatographic separation was performed on a BEH C18 1.7μm, 2.1mm×50mm column, settled at 50°C, with a gradient run of two mobile phases: ammonium acetate 5mM (pH 9.5) and acetonitrile, with a flow rate of 0.4mL/min for 5min. Tandem-mass detection was carried out in positive electrospray ionization mode. Both inter and intraday imprecision and inaccuracy were below 15%, as required by FDA guidelines, while both recoveries and matrix effects resulted within the acceptance criteria. The method was tested on 80 patients samples with good performance. Being robust, simple and fast and requiring a low plasma volume, this method resulted eligible for a clinical routine use. PMID:27131146

  8. Studi senesi, Facoltà di Giurisprudenza, Università degli studi di Siena, CXXII, III.ª Serie, LIX, fasc. 1, Siena, 2010, 181 págs.

    OpenAIRE

    Manuel J Peláez

    2012-01-01

    Studi senesi publica en el presente fascículo parte del homenaje que se le ha dedicado al prestigioso romanista Remo Martini, en el Aula Magna de la Universidad de Siena el 27 de abril de 2010, con las intervenciones de Stefania Pietrini y de Emanuele Stolfi. Roberta Alonzi demuestra un conocimiento muy preciso de la bibliografía británica y francesa sobre el significado diplomático del contencioso de Fascioda en Egipto en 1898. Alberto Ferrara analiza la crisis financiera en Estados Unidos e...

  9. Salustiano de Dios y Eugenia Torijano (coords.), Cultura, política y práctica del Derecho. Juristas de Salamanca, Siglos XV-XX, Ediciones Universidad de Salamanca, Salamanca, 2012, 567 págs.

    OpenAIRE

    Guillermo Hierrezuelo Conde

    2013-01-01

    En esta obra se recogen hasta siete estudios relativos a juristas de Salamanca en el período que comprende desde el s. XV hasta el pasado siglo XX. El primero de ellos está dedicado a distintos juristas salmantinos que vivieron durante el s. XV, destacando la figura de Pedro de Luna, conocido como papa Benedicto XIII; el segundo de los artículos pone de relieve la importancia de los colegios mayores salmantinos, institución en la que se tramitaron numerosos expedientes a los colegiales para l...

  10. Experimental and numerical investigations of the creep behaviour of the dissimilar weldment GS-17 CrMoV 5 11 and X 20 CrMoV 12 1

    Energy Technology Data Exchange (ETDEWEB)

    Buchmayr, B.; Cerjak, H.; Witwer, M. (Technische Univ., Graz (Austria). Abt. fuer Werkstoffkunde und Schweisstechnik); Maile, K.; Theofel, H.; Eckert, W. (Stuttgart Univ. (Germany, F.R.). Staatliche Materialpruefungsanstalt)

    1990-01-01

    In the collaborative programme between MPA, Stuttgart, and the Institute for Materials Science and Welding Technology at the Technical University, Graz, within the framework of COST 505 'Materials for Steam Turbines', the creep-rupture behaviour of teh dissimilar weldment 1% CrMoV/12% CrMoV was investigated. Independent of the effects given by the welding process, weld metal and post weld heat treatment, the fine-grained zone in the HAZ of the lower alloyed steel grade was life-determining after long-time creep loading. The decarburized coarse grained HAZ near the fusion line is critical at higher stress levels, e.g. breakdowns. (orig.).

  11. Experimental and numerical investigations of the creep behaviour of the dissimilar weldment GS-17 CrMoV 5 11 and X 20 CrMoV 12 1

    International Nuclear Information System (INIS)

    In the collaborative programme between MPA, Stuttgart, and the Institute for Materials Science and Welding Technology at the Technical University, Graz, within the framework of COST 505 'Materials for Steam Turbines', the creep-rupture behaviour of teh dissimilar weldment 1% CrMoV/12% CrMoV was investigated. Independent of the effects given by the welding process, weld metal and post weld heat treatment, the fine-grained zone in the HAZ of the lower alloyed steel grade was life-determining after long-time creep loading. The decarburized coarse grained HAZ near the fusion line is critical at higher stress levels, e.g. breakdowns. (orig.)

  12. Utilització del software constructiviste Etoys com a ajuda i complement als cicles formatius de GM i GS d’automatització Industrial. Unitat Formativa: Máquines Elèctriques

    OpenAIRE

    Mañas García, Santiago

    2011-01-01

    Castellà: Squeak o Etoys, su versión para el proyecto One Laptop per Child (OLPC), es un software Open Source desarrollado especialmente con fines educativos, en la linea de LOGO de Seymour Papert, siguiendo las teorias constructivistas de Piaget y las investigaciones de Jerome Bruner. Es un entorno de programación multimedia pensado especialmente para que el alumno pueda ser protagonista de su propio aprendizaje. Tradicionalmente ha venido utilizandose en Primaria y Secundaria...

  13. 无壳瓜子挥发油化学成分的气相色谱-质谱分析%Study on chemical components of volatile oil from hulless pumpkin seeds based on GS-MS method

    Institute of Scientific and Technical Information of China (English)

    李玉琴; 郭玉蓉; 刘磊; 齐永秀; 贾宝秀; 李珂; 刘彩红; 曹明亮

    2006-01-01

    用毛细管气相色谱法分离无壳瓜子中的挥发油组分,用GC-MS和Kvoat's指数法对各种组分进行鉴定,共定性检测出34种化合物,占挥发油总含量的73.5 %,其中主要成分为甲酸乙酯、1-十一炔、2,8-二甲基十一酸甲酯、正十八烷、十六酸甲酯、十八二烯-11,14-酸甲酯、正十八酸乙酯和十八二烯-9,12酸(z,z)等.

  14. 用食品BDI-GS体系综合评价牛奶的营养健康效应%Comprehensive evaluation on nutrition and health of bovine milk using food BDI-GS system

    Institute of Scientific and Technical Information of China (English)

    龙伟; 沈秀; 王德芝; 王浩; 王晓光; 白佳利; 周则卫

    2013-01-01

    In this issue,study functions and safety of pure bovine milk through mice trial,establish a novel comprehensive system and method to evaluate nutrition,health-promotion and safety of food.The ICR male mice during growth were used in the trial,the experimental mice were feed with low nutrient maize and blending bovine milk feeds for 12 days,display and evaluate the effects of bovine milk with animal experiment data through observing and statistics the mice body weights,organ weights,organ indexes and its effect Benefit-damage Indexes (BDI).The study demonstrated that bovine milk possesses comprehensive and overall nutrition functions,certain health-promoting and foodtherapy functions for diabetes mellitus,and attenuating obesity effect,but some adverse response to bone mass.Bovine milk intake fits for nutrition of growing and nourishing for children and adolescents,nevertheless,drinking for a long period in large amount is not suitable for preventing adults and olds osteoporosis.The evaluation system has intuition,concrete,overall and comprehension features.This method can be used to evaluate nutrition,health-promotion and safety for various foods.%通过建立的食品功效安全BDI-GS评价体系和低营养小鼠模型方法综合评价纯牛奶的营养及健康效应.实验用生长期ICR雄性小鼠,通过12 d低营养玉米饲料和5%,10%及15%纯牛奶掺和饲料喂养,考察统计小鼠体质量、脏器质量、系数及其效应的损益指数(BDI)等指标,用动物实验数据评价牛奶功效.结果表明,牛奶的营养功效综合、全面;牛奶对II型糖尿病有一定的预防保健功效及控制体重(减肥)作用,但对骨质健康影响轻微.牛奶适合儿童、青少年生长发育饮用,适量饮用对中老年健康有益,而长期大量饮用对中老年防治骨质疏松不利.

  15. Salustiano de Dios y Eugenia Torijano (coords., Cultura, política y práctica del Derecho. Juristas de Salamanca, Siglos XV-XX, Ediciones Universidad de Salamanca, Salamanca, 2012, 567 págs.

    Directory of Open Access Journals (Sweden)

    Guillermo Hierrezuelo Conde

    2013-03-01

    Full Text Available En esta obra se recogen hasta siete estudios relativos a juristas de Salamanca en el período que comprende desde el s. XV hasta el pasado siglo XX. El primero de ellos está dedicado a distintos juristas salmantinos que vivieron durante el s. XV, destacando la figura de Pedro de Luna, conocido como papa Benedicto XIII; el segundo de los artículos pone de relieve la importancia de los colegios mayores salmantinos, institución en la que se tramitaron numerosos expedientes a los colegiales para la averiguación de la limpieza de sangre, así como otros aspectos, como la honorabilidad de la línea familiar; la tercera de las colaboraciones gira en torno a la figura del oficial de la administración española en el s. XVIII, y a las referencias que sobre ella se recogen en la obra de Jean Pierre Dedieu titulada La muerte del letrado; el cuarto estudia la personalidad del catedrático Vicente Fernández de Ocampo, que vivió entre 1743 y 1815; el quinto al informe realizado al proyecto de Código Civil de 1851 por la Facultad de jurisprudencia de la Universidad de Salamanca, que aunque no llegó a entrar en vigor adquirió gran trascendencia en el ámbito civil; el sexto, a la importancia de las audiencias de lo criminal de Salamanca y Ciudad Rodrigo, hasta la supresión de esta última; y el último de ellos, a aquellos profesores de la Universidad de Salamanca que vivieron los primeros años de la Dictadura franquista, entre los que podemos citar a Teodoro Andrés Marcos y Nicolás Rodríguez Aniceto, entre otros.

  16. Reseña de: Jonathan Barnes, The Presocratic Philosophers; G.S. Kirk, J.E. Raven & M. Schofield, The Presocratic Philosophers; Charles H. Kahn, The Art and Thought of Heraclitus

    OpenAIRE

    Peña, Lorenzo

    1986-01-01

    Los tres libros abordan problemas importantes de la historia de la filosofía griega a través de la filosofía analítica. Los tres tratan de ofrecer interpretaciones de los presocráticos que eviten atribuirles contradicciones; y los tres fracasan en ese intento hermenéutico, aplicando una caridad interpretativa innecesaria. Y es que es racional reconocer grados de existencia y afirmar que hay contradicciones verdaderas.

  17. Manuel J. Peláez (dir., Diccionario de Canonistas y Eclesiasticistas Europeos y Americanos (Tomo I, AV Akademikerverlag GmbH & Co. KG, EAE, Saarbrücken, 2012, 547 págs.

    Directory of Open Access Journals (Sweden)

    Purificación Alcaide-Pulido

    2013-03-01

    Full Text Available ¿Por qué se otorga escasa atención a los canonistas y eclesiasticistas dentro del Derecho canónico y del Derecho eclesiástico? Esta es la cuestión que surge y el motivo de la aparición de la obra Diccionario de canonistas y eclesiasticistas europeos y americanos. Dicha obra aparece como una necesidad científica emanada de la escasa atención que en el ámbito citado se presta a canonistas y a eclesiasticistas, a través de una recopilación de los pensadores más influyentes en las diversas áreas que comprenden el Derecho Canónico y Eclesiástico, a lo largo del último milenio y, por ende, también de interés para iushistoricistas.

  18. Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA - Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis

    DEFF Research Database (Denmark)

    Hatt, Lotte; Aagaard, Mads M; Graakjaer, Jesper;

    2015-01-01

    Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different chromosomes in a maternal blood sample. The aim of...... the present study was to define new fetal specific epigenetic markers present in placental DNA that can be utilized in non-invasive prenatal diagnosis. We have conducted a high-resolution methylation specific beadchip microarray study assessing more than 450.000 CpG sites. We have analyzed the DNA...... with a potential for aneuploidy testing as well as a list of markers for regions harboring sub-microscopic deletion- or duplication syndromes....

  19. Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

    Czech Academy of Sciences Publication Activity Database

    Horák, Daniel; Plichta, Zdeněk; Starykovych, M.; Myronovskij, S.; Kit, Y.; Chopyak, V.; Stoika, R.

    2015-01-01

    Roč. 5, č. 77 (2015), s. 63050-63055. ISSN 2046-2069 R&D Projects: GA MŠk(CZ) 7E12053 EU Projects: European Commission(XE) 246513 - NADINE Institutional support: RVO:61389013 Keywords : magnetic * systemic lupus erythematosis * histone Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.840, year: 2014

  20. Comment on 'Clay mineralogy of the Pelagic sediments: along a west-east transect in the Indian Ocean by G.S. Roonwal and S.K. Srivastava' Journal of the Geological Society of India 38; 1991; 37-54'

    Digital Repository Service at National Institute of Oceanography (India)

    Rao, V.P.

    stream_size 2 stream_content_type text/plain stream_name J_Geol_Soc_India_38_539.pdf.txt stream_source_info J_Geol_Soc_India_38_539.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  1. Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis.

    Directory of Open Access Journals (Sweden)

    Lotte Hatt

    Full Text Available Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different chromosomes in a maternal blood sample. The aim of the present study was to define new fetal specific epigenetic markers present in placental DNA that can be utilized in non-invasive prenatal diagnosis. We have conducted a high-resolution methylation specific beadchip microarray study assessing more than 450.000 CpG sites. We have analyzed the DNA methylation profiles of 10 maternal blood samples and compared them to 12 1st trimesters chorionic samples from normal placentas, identifying a number of CpG sites that are differentially methylated in maternal blood cells compared to chorionic tissue. To strengthen the utility of these differentially methylated CpG sites to be used with methyl-sensitive restriction enzymes (MSRE in PCR-based NIPD, we furthermore refined the list of selected sites, containing a restriction sites for one of 16 different methylation-sensitive restriction enzymes. We present a list of markers on chromosomes 13, 18 and 21 with a potential for aneuploidy testing as well as a list of markers for regions harboring sub-microscopic deletion- or duplication syndromes.

  2. Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA – Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis

    OpenAIRE

    Hatt, Lotte; Aagaard, Mads M.; Graakjaer, Jesper; Bach, Cathrine; Sommer, Steffen; Inge E Agerholm; Kølvraa, Steen; Bojesen, Anders

    2015-01-01

    Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different chromosomes in a maternal blood sample. The aim of the present study was to define new fetal specific epigenetic markers present in placental DNA that can be utilized in non-invasive prenatal diagnosis. We have conducted a high-resolution methylati...

  3. Model Regulations for the Use of Radiation Sources and for the Management of the Associated Radioactive Waste. Supplement to IAEA Safety Standards Series No. GS-G-1.5

    International Nuclear Information System (INIS)

    IAEA Safety Standards Series No. GSR Part 1, Governmental, Legal and Regulatory Framework for Safety, requires that governments establish laws and statutes to make provisions for an effective governmental, legal and regulatory framework for safety. The framework for safety includes the establishment of a regulatory body. The regulatory body has the authority and responsibility for promulgating regulations, and for preparing their implementation. This publication provides advice on an appropriate set of regulations covering all aspects of the use of radiation sources and the safe management of the associated radioactive waste. The regulations provide the framework for the regulatory requirements and conditions to be incorporated into individual authorizations for the use of radiation sources in industry, medical facilities, research and education and agriculture. The regulations also establish criteria to be used for assessing compliance. This publication allows States to appraise the adequacy of their existing regulations and regulatory guides, and can be used as a reference for those States developing regulations for the first time. The regulations set out in this publication will need to be adapted to take account of local conditions, technical resources and the scale of facilities and activities in the State. The set of regulations in this publication is based on the requirements established in the IAEA safety standards series, in particular in IAEA Safety Standards Series No. GSR Part 3 (Interim), Radiation Protection and Safety of Radiation Sources: International Basic Safety Standards, in IAEA Safety Standards Series No. GSR Part 5, Predisposal Management of Radioactive Waste, and in IAEA Safety Standards Series No. SSR-5, Disposal of Radioactive Waste. They are also derived from the Code of Conduct of the Safety and Security of Radiation Sources and the Guidance on the Import and Export of Radioactive Sources. This publication allows States to appraise the adequacy of their existing regulations and regulatory guides

  4. Philippe Ariès. Morir en Occidente: desde la Edad Media hasta nuestros días : Adriana Hidalgo Editora. Buenos Aires, 2007, 272 págs.

    OpenAIRE

    Bruzzone, Daiana

    2008-01-01

    Ligadas cada una a su contexto, las concepciones de la muerte configuran modos de convivencia social. A pesar de ello, las ciencias sociales son cómplices de su época, sostiene el autor, y hacen del tema uno de los mayores tabúes de nuestra sociedad contemporánea. En esta era postindustrial la muerte se encuentra prohibida en las prácticas cotidianas, de manera que detenerse allí puede resultar trivial para los intelectuales –dice Ariès-, quienes se ven mejor seducidos por su aspecto demográf...

  5. Gyromagnetic g_s factors of the spin-1/2 particles in the (1/2+,1/2-,3/2-) triad of the four-vector spinor, \\psi_\\mu, irreducibility, and linearity

    CERN Document Server

    Acosta, E G Delgado; Kirchbach, M

    2015-01-01

    We show that the spin (1/2-) particle from the (1/2,1)+(1,1/2) Lorentz irreducible sector of the four-vector spinor can not be described within a linear formalism but behaves as a genuinely quadratic fermion satisfying the generalized Feynman-Gell-Mann equation with a gyromagnetic factor of (-2/3). In contrast, spin (1/2 +) from the (1/2,0)+(0,1/2) sector is confirmed as a genuine linear Dirac fermion whose gyromagnetic factor takes the value of two units.We calculate Compton scatterings off each one of the two targets and obtain both times well behaved cross sections in the ultra relativistic limit and in accord with unitarity.

  6. Gerðardómar í fjárfestingardeilum milli ríkja og erlendra fjárfesta. Álitaefni um lögsögu ICSID stofnunarinnar

    OpenAIRE

    Axel Ingi Magnússon 1988

    2014-01-01

    Markmið þessarar ritgerðar tvíþætt: Í fyrsta lagi að gera grein fyrir því hvað felist almennt í gerðardómsmeðferð og þá sérstaklega á deilum milli ríkis og erlenda fjárfesta og um leið fjalla um þær stofnanir og þá þjóðaréttarlegu samninga sem hafa áhrif við úrlausn þessara deilna. Verður sjónum þá sérstaklega beint að ICSID stofnuninni en flest gerðardómsmál á þessu sviði fara fram á grundvelli ICSID samningsins. Í öðru lagi verður sérstaklega tekin til skoðunar þau skilyrði sem ICSID samnin...

  7. Purification and Properties of a Prokaryote Type Glutamine Synthetase from the Bialaphos Producer Streptomyces hygroscopicus SF1293

    NARCIS (Netherlands)

    Kumada, Yoichi; Takano, Eriko; Nagaoka, Kozo

    1990-01-01

    A prokaryote type glutamine synthetase (GS) was purified from a bialaphos (BA)-producing organism, Streptomyces hygroscopicus SF1293 (SF1293). The GS (GS I) consisted of a 55,000 dalton subunit, and its N-terminal amino acid sequence was similar to that of S. coelicolor GS. GS I was highly sensitive

  8. Theory of optical flashes

    International Nuclear Information System (INIS)

    The theory of optical flashes created by x- and γ-ray burst heating of stars in binaries is reviewed. Calculations of spectra due to steady-state x-ray reprocessing and estimates of the fundamental time scales for the non-steady case are discussed. The results are applied to the extant optical data from x-ray and γ-ray bursters. Finally, I review predictions of flashes from γ-ray bursters detectable by a state of the art all-sky optical monitor

  9. Registration of N619 to N640 grain sorghum lines with waxy or wild-type endosperm

    Science.gov (United States)

    Sorghum [Sorghum bicolor (L.) Moench] lines A N619 to A N636 (Reg. No. GS-XXX, PI 670134 to Reg. No. GS-XXX, PI 670151); B N619 to B N636 (Reg. No. GS-XXX, PI 671777 to Reg. No. GS-XXX, PI 671794); and R N637 to R N640 (Reg. No. GS-XXX, PI 670152 to Reg. No. GS-XXX, PI 670155) comprise nine pairs of...

  10. Repercussion of mesophyll-specific overexpression of a soybean cytosolic glutamine synthetase gene in alfalfa (Medicago sativa L.) and tobacco (Nicotiana tabaccum L.)

    OpenAIRE

    Seger, Mark; Ortega, Jose Luis; Bagga, Suman; Gopalan, Champa-Sengupta

    2009-01-01

    Glutamine synthetase (GS) plays a central role in plant nitrogen metabolism. Plant GS occurs as a number of isoenzymes present in either the cytosol (GS1) or chloroplast/plastid (GS2). There are several reports of improved performance in transgenic plants overexpressing GS1 transgenes driven by the constitutive CaMV35S promoter. Improvement has been attributed to the GS1 transgene product functioning to enhance re-assimilation of NH4+ released by photorespiration or protein degradation. In th...

  11. INTEGRAL finds renewed X-ray activity of the Neutron star X-ray transient SAX J1750.8-2900

    DEFF Research Database (Denmark)

    Sanchez-Fernandez, Celia; Chenevez, Jérôme; Kuulkers, Erik;

    2015-01-01

    INTEGRAL Galactic bulge monitoring observations (ATel #438) on UT 13 September 2015 18:50-22:32 reveal renewed X-ray activity from the low-mass X-ray binary transient and Type I X-ray burster SAX J1750.8-2900 (IAU Circ. #6597). The last outburst from this source was reported in 2011 (ATels #3170,...

  12. Hubble Space Telescope STIS Observations of GRB 000301C: CCD Imaging and Near-Ultraviolet MAMA Spectroscopy

    NARCIS (Netherlands)

    A. Smette; A.S. Fruchter; Th.R. Gull; K.C. Sahu; L. Petro; H. Ferguson; J. Rhoads; D.J. Lindler; R.A.M.J. Wijers

    2001-01-01

    We present Space Telescope Imaging Spectrograph observations of the optical transient (OT) counterpart of the gamma-ray burster GRB 000301C obtained 5 days after the burst, on 2000 March 6. CCD clear-aperture imaging reveals a R~=21.50+/-0.15 source with no apparent host galaxy. An 8000 s, 1150 Å

  13. Bifurcation of a Saddle-Node Limit Cycle with Homoclinic Orbits Satisfying the Small Lobe Condition in a Leech Neuron Model

    Institute of Scientific and Technical Information of China (English)

    YOOER Chi-Feng; XU Jian-Xue; ZHANG Xin-Hua

    2009-01-01

    Mechanism of period-adding cascades with chaos in a reduced leech neuron model is suggested as the bifurcation of a saddle-node limit cycle with homoclinic orbits satisfying the "small lobe condition",instead of the blue-sky catastrophe.In every spiking adding,the new spike emerges at the end of the spiking phase of the bursters.

  14. GENERAL: Bifurcation of a Saddle-Node Limit Cycle with Homoclinic Orbits Satisfying the Small Lobe Condition in a Leech Neuron Model

    Science.gov (United States)

    Yooer, Chi-Feng; Xu, Jian-Xue; Zhang, Xin-Hua

    2009-08-01

    Mechanism of period-adding cascades with chaos in a reduced leech neuron model is suggested as the bifurcation of a saddle-node limit cycle with homoclinic orbits satisfying the “small lobe condition", instead of the blue-sky catastrophe. In every spiking adding, the new spike emerges at the end of the spiking phase of the bursters.

  15. Hubble Space Telescope STIS observations of GRB 000301C: CCD imaging and near-ultraviolet MAMA spectroscopy

    DEFF Research Database (Denmark)

    Smette, A.; Fruchter, A.S.; Gull, T.R.;

    2001-01-01

    We present Space Telescope Imaging Spectrograph observations of the optical transient (OT) counterpart of the c-ray burster GRB 000301C obtained 5 days after the burst, on 2000 March 6. CCD clear-aperture imaging reveals a R similar or equal to 21.50 +/- 0.15 source with no apparent host galaxy. ...

  16. The dance of the double stars

    International Nuclear Information System (INIS)

    The paper concerns pairs of stars orbiting one another. The evolutionary path model for close binary stars, involving a mass transfer of gases between the stars, is described. The life history of a single star; cataclysmic variables; the algol paradox, matter and lagranges' point; x-ray binaries and bursters; and pulsars; are all briefly discussed. (U.K.)

  17. Dance of the double stars

    Energy Technology Data Exchange (ETDEWEB)

    Theokas, A.

    1985-09-19

    The paper concerns pairs of stars orbiting one another. The evolutionary path model for close binary stars, involving a mass transfer of gases between the stars, is described. The life history of a single star; cataclysmic variables; the algol paradox, matter and lagranges' point; x-ray binaries and bursters; and pulsars; are all briefly discussed.

  18. INTEGRAL confirms the detection of renewed activity from the NS transient H 1658-298

    DEFF Research Database (Denmark)

    Sanchez-Fernandez, C.; Eckert, D.; Bozzo, E.;

    2015-01-01

    Crab, 4.5-sigma significance in the 20-40 keV energy band) by IBIS, the Imager Onboard the INTEGRAL Satellite. H 1658-298 is a known X-ray burster. Therefore we have searched for Type-I burst activity in the JEM-X data during these observations, but no indications of burst activity have been found. We...

  19. Optimization of Swine Breeding Programs Using Genomic Selection with ZPLAN.

    Science.gov (United States)

    Lopez, B M; Kang, H S; Kim, T H; Viterbo, V S; Kim, H S; Na, C S; Seo, K S

    2016-05-01

    The objective of this study was to evaluate the present conventional selection program of a swine nucleus farm and compare it with a new selection strategy employing genomic enhanced breeding value (GEBV) as the selection criteria. The ZPLAN+ software was employed to calculate and compare the genetic gain, total cost, return and profit of each selection strategy. The first strategy reflected the current conventional breeding program, which was a progeny test system (CS). The second strategy was a selection scheme based strictly on genomic information (GS1). The third scenario was the same as GS1, but the selection by GEBV was further supplemented by the performance test (GS2). The last scenario was a mixture of genomic information and progeny tests (GS3). The results showed that the accuracy of the selection index of young boars of GS1 was 26% higher than that of CS. On the other hand, both GS2 and GS3 gave 31% higher accuracy than CS for young boars. The annual monetary genetic gain of GS1, GS2 and GS3 was 10%, 12%, and 11% higher, respectively, than that of CS. As expected, the discounted costs of genomic selection strategies were higher than those of CS. The costs of GS1, GS2 and GS3 were 35%, 73%, and 89% higher than those of CS, respectively, assuming a genotyping cost of $120. As a result, the discounted profit per animal of GS1 and GS2 was 8% and 2% higher, respectively, than that of CS while GS3 was 6% lower. Comparison among genomic breeding scenarios revealed that GS1 was more profitable than GS2 and GS3. The genomic selection schemes, especially GS1 and GS2, were clearly superior to the conventional scheme in terms of monetary genetic gain and profit. PMID:26954222

  20. Optimization of Swine Breeding Programs Using Genomic Selection with ZPLAN+

    Science.gov (United States)

    Lopez, B. M.; Kang, H. S.; Kim, T. H.; Viterbo, V. S.; Kim, H. S.; Na, C. S.; Seo, K. S.

    2016-01-01

    The objective of this study was to evaluate the present conventional selection program of a swine nucleus farm and compare it with a new selection strategy employing genomic enhanced breeding value (GEBV) as the selection criteria. The ZPLAN+ software was employed to calculate and compare the genetic gain, total cost, return and profit of each selection strategy. The first strategy reflected the current conventional breeding program, which was a progeny test system (CS). The second strategy was a selection scheme based strictly on genomic information (GS1). The third scenario was the same as GS1, but the selection by GEBV was further supplemented by the performance test (GS2). The last scenario was a mixture of genomic information and progeny tests (GS3). The results showed that the accuracy of the selection index of young boars of GS1 was 26% higher than that of CS. On the other hand, both GS2 and GS3 gave 31% higher accuracy than CS for young boars. The annual monetary genetic gain of GS1, GS2 and GS3 was 10%, 12%, and 11% higher, respectively, than that of CS. As expected, the discounted costs of genomic selection strategies were higher than those of CS. The costs of GS1, GS2 and GS3 were 35%, 73%, and 89% higher than those of CS, respectively, assuming a genotyping cost of $120. As a result, the discounted profit per animal of GS1 and GS2 was 8% and 2% higher, respectively, than that of CS while GS3 was 6% lower. Comparison among genomic breeding scenarios revealed that GS1 was more profitable than GS2 and GS3. The genomic selection schemes, especially GS1 and GS2, were clearly superior to the conventional scheme in terms of monetary genetic gain and profit. PMID:26954222

  1. Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii.

    Science.gov (United States)

    Chen, Q; Silflow, C D

    1996-11-01

    To elucidate the role of glutamine synthetase (GS) in nitrogen assimilation in the green alga Chlamydomonas reinhardtii we used maize GS1 (the cytosolic form) and GS2 (the chloroplastic form) cDNAs as hybridization probes to isolate C. reinhardtii cDNA clones. The amino acid sequences derived from the C. reinhardtii clones have extensive homology with GS enzymes from higher plants. A putative amino-terminal transit peptide encoded by the GS2 cDNA suggests that the protein localizes to the chloroplast. Genomic DNA blot analysis indicated that GS1 is encoded by a single gene, whereas two genomic fragments hybridized to the GS2 cDNA probe. All GS2 cDNA clones corresponded to only one of the two GS2 genomic sequences. We provide evidence that ammonium, nitrate, and light regulate GS transcript accumulation in green algae. Our results indicate that the level of GS1 transcripts is repressed by ammonium but induced by nitrate. The level of GS2 transcripts is not affected by ammonium or nitrate. Expression of both GS1 and GS2 genes is regulated by light, but perhaps through different mechanisms. Unlike in higher plants, no decreased level of GS2 transcripts was detected when cells were grown under conditions that repress photorespiration. Analysis of GS transcript levels in mutants with defects in the nitrate assimilation pathway show that nitrate assimilation and ammonium assimilation are regulated independently. PMID:8938407

  2. Characterization and Anti-tumor Activity of Giycopeptides from Ganoderma sinensis

    Institute of Scientific and Technical Information of China (English)

    GAO Yang; JIANG Ru-zhi; CHEN Ying-hong; LUO Hao-ming; XU Duo-duo; GAO Qi-pin

    2009-01-01

    The water-soluble part(GS) of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecu-Iar(GS-H) and low molecular(GS-L) parts by Cellulose Super Filtration, and GS was also fractionated into four frac-tions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-I, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000,GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and me-thylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fueosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2,1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.

  3. Glutamine Synthetase in Legumes: Recent Advances in Enzyme Structure and Functional Genomics

    OpenAIRE

    Marco Betti; Margarita García-Calderón; Pérez-Delgado, Carmen M.; Alfredo Credali; Guillermo Estivill; Francisco Galván; Vega, José M.; Márquez, Antonio J.

    2012-01-01

    Glutamine synthetase (GS) is the key enzyme involved in the assimilation of ammonia derived either from nitrate reduction, N2 fixation, photorespiration or asparagine breakdown. A small gene family is encoding for different cytosolic (GS1) or plastidic (GS2) isoforms in legumes. We summarize here the recent advances carried out concerning the quaternary structure of GS, as well as the functional relationship existing between GS2 and processes such as nodulation, photore...

  4. Study of the high energy emission of accreting compact objects with SPI/INTEGRAL

    International Nuclear Information System (INIS)

    observed spectral variability in the framework of a new Comptonization model which accounts self-consistently for the presence of a magnetic field and introduce a purely non-thermal scenario as an alternative interpretation of the luminous hard state of accreting black hole binaries. Finally, I present a long term study of the high energy emission of the X-ray burster GS 1826-24. The accretion flow being extraordinarily stable, I integrated over 8 Msec of data allowing to measure the average source spectrum up to 500 keV. Once again, there is strong evidence for a hard spectral tail above 150 keV, establishing that this feature is not exclusively associated to black hole systems. I compare the results obtained for the three sources and discuss the possible physical origins of the high energy emission of stellar-size compact objects, emphasizing that all observed spectral shapes can be explained by a non-thermal magnetized corona model. (author)

  5. Rehabilitation of spillway and intake guides and gates

    International Nuclear Information System (INIS)

    Operating problems are fairly common at some of the ageing vertical lift intake and spillway gates at many of the older hydroelectric generating stations (GS) in Canada. The problems result from deteriorated or corroded gate guides and roller assemblies. In 1989, the KGS Group reviewed several viable, cost-effective options for rehabilitation. The remedial measures have since been used by several Canadian utilities including Manitoba Hydro, Hydro-Quebec, Ontario Hydro, SaskPower and Winnipeg Hydro. Projects currently underway include: Beauharnois GS intake, Kelsey GS spillway, McArthur GS spillway, Slave Falls GS intake, Island Falls GS intake, Chenaux GS intake, Abitibi Canyon GS intake and Des Joachims GS intake. This paper presents the results of the inspection/assessment of gates and guides and provides details of the overall concepts and rehabilitation methods for the gate and intake guide repairs being implemented at each of these stations. 5 refs., 1 tab., 6 figs

  6. Forced bursting and transition mechanism in CO oxidation with three time scales

    Institute of Scientific and Technical Information of China (English)

    Li Xiang-Hong; Bi Qin-Sheng

    2013-01-01

    The mathematical model of CO oxidation with three time scales on platinum group metals is investigated,in which order gaps between the time scales related to external perturbation and the rates associated with different chemical reaction steps exist.Forced bursters,such as point-point type forced bursting and point-cycle type forced bursting,are presented.The bifurcation mechanism of forced bursting is novel,and the phenomenon where two different kinds of spiking states coexist in point-cycle type forced bursting has not been reported in previous work.A double-parameter bifurcation set of the fast subsystem is explored to reveal the transition mechanisms of different forced bursters with parameter variation.

  7. Forced bursting and transition mechanism in CO oxidation with three time scales

    International Nuclear Information System (INIS)

    The mathematical model of CO oxidation with three time scales on platinum group metals is investigated, in which order gaps between the time scales related to external perturbation and the rates associated with different chemical reaction steps exist. Forced bursters, such as point—point type forced bursting and point—cycle type forced bursting, are presented. The bifurcation mechanism of forced bursting is novel, and the phenomenon where two different kinds of spiking states coexist in point—cycle type forced bursting has not been reported in previous work. A double-parameter bifurcation set of the fast subsystem is explored to reveal the transition mechanisms of different forced bursters with parameter variation. (general)

  8. Kinetics of borided gear steels

    Indian Academy of Sciences (India)

    Ibrahim Gunes

    2013-06-01

    In this study, the case properties and diffusion kinetics of GS18NiMoCr36 (GS18), GS22NiMoCr56 (GS22) and GS32NiCrMo6.4 (GS32) gear steels borided in Ekabor-II powder were investigated by conducting a series of experiments at temperatures of 1123, 1173 and 1223 K for 2, 4 and 6 h. The boride layer was characterized by optical microscopy, X-ray diffraction technique and microVickers hardness tester. X-ray diffraction analysis of boride layers on the surface of the steels revealed the existence of FeB, Fe2B, CrB and Cr2B compounds. The thickness of the boride layer increases by increasing boriding time and temperature for all steels. The hardness of the boride compounds formed on the surface of the steels GS18, GS22 and GS32 ranged from 1624 to 1905 HV0,05, 1702 to 1948 HV0,05, and 1745 to 2034 HV0,05 respectively, whereas Vickers hardness values of the untreated steels GS18, GS22 and GS32 were 335 HV0,05, 358 HV0,05 and 411 HV0,05, respectively. The activation energies (Q) of borided steels were 228.644 kJ/mol for GS18, 280.609 kJ/mol for GS22 and 294.359 kJ/mol for GS32. The growth kinetics of the boride layers forming on the GS18, GS22 and GS32 steels and the thickness of boride layers were also investigated.

  9. Mixed-mode oscillations in a multiple time scale phantom bursting system

    OpenAIRE

    Krupa, Maciej; Vidal, Alexandre; Desroches, Mathieu; Clément, Frédérique

    2012-01-01

    38 pages, 16 figures. International audience In this work we study mixed mode oscillations in a model of secretion of GnRH (Gonadotropin Releasing Hormone). The model is a phantom burster consisting of two feedforward coupled FitzHugh-Nagumo systems, with three time scales. The forcing system (Regulator) evolves on the slowest scale and acts by moving the slow nullcline of the forced system (Secretor). There are three modes of dynamics: pulsatility (transient relaxation oscillation), su...

  10. A Ritzian interpretation of variable stars

    Science.gov (United States)

    Fritzius, Robert S.

    2000-09-01

    A revived version of de Sitter's 1913 ``binary stars'' argument against Ritz's Galilean relativity general electrodynamic theory provides a means of explaining of the mechanism underlying the apparent variability of variable stars, pulsars, and gamma-ray bursters. Numerical code to compute c+v induced intensity peaks and blueshift chirps for spectroscopic binaries provides graphical displays for comparison with observed light curves and spectra for different classes of variable stars. .

  11. Inelastic Branch of the Stellar Reaction $^{14}$O$(\\alpha,p)^{17}$F

    CERN Multimedia

    Hass, M; Van duppen, P L E

    2002-01-01

    We propose to use the upgraded REX-ISOLDE beam energy to study the astrophysically important $^{14}$O($\\alpha$, p)$^{17}$F reaction in time reverse kinematics. In particular, we will use the highly efficient miniball + CD detection system to measure the previously undetermined inelastic proton branch of the 1$^-$ state at 6.15 MeV in $^{18}$Ne. This state dominates the reaction rate under X-ray burster conditions.

  12. How to Tell a Jet from a Balloon: A Proposed Test for Beaming in Gamma Ray Bursts

    OpenAIRE

    Rhoads, James E.

    1997-01-01

    If gamma ray bursts are highly collimated, the energy requirements of each event may be reduced by several (~ 4-6) orders of magnitude, and the event rate increased correspondingly. Extreme conditions in gamma ray bursters lead to highly relativistic motions (bulk Lorentz factors Gamma > 100). This results in strong forward beaming of the emitted radiation in the observer's rest frame. Thus, all information on gamma ray bursts comes from those ejecta emitted in a narrow cone (opening angle 1/...

  13. Inferring network properties of cortical neurons with synaptic coupling and parameter dispersion

    OpenAIRE

    Dipanjan eRoy; Viktor eJirsa

    2013-01-01

    Computational models at different space-time scales allow us to understand the fundamental mechanisms that govern neural processes and relate uniquely these processes to neuroscience data. In this work, we propose a novel neurocomputational unit (a mesoscopic model which tell us about the interaction between local cortical nodes in a large scale neural mass model) of bursters that qualitatively captures the complex dynamics exhibited by a full network of parabolic bursting neurons. We observe...

  14. A Search for Astrophysical Burst Signals at the Sudbury Neutrino Observatory

    CERN Document Server

    Aharmim, B; Anthony, A E; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox, G A; Dai, X; Deng, H; Detwiler, J A; DiMarco, M; Diamond, M D; Doe, P J; Doucas, G; Drouin, P -L; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Gagnon, N; Goon, J TM; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Harvey, P J; Hazama, R; Heintzelman, W J; Heise, J; Helmer, R L; Hime, A; Howard, C; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Jerkins, M; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Kraus, C; Krauss, C B; Krueger, A; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Levine, I; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McCauley, N; McDonald, A B; McGee, S R; Miller, M L; Monreal, B; Monroe, J; Nickel, B G; Noble, A J; O'Keeffe, H M; Oblath, N S; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, D; Skensved, P; Sonley, T J; Stonehill, L C; Tesic, G; Tolich, N; Tsui, T; Van Berg, R; VanDevender, B A; Virtue, C J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wark, D L; Watson, P J S; Wendland, J; West, N; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2013-01-01

    The Sudbury Neutrino Observatory (SNO) has confirmed the standard solar model and neutrino oscillations through the observation of neutrinos from the solar core. In this paper we present a search for neutrinos associated with sources other than the solar core, such as gamma-ray bursters and solar flares. We present a new method for looking for temporal coincidences between neutrino events and astrophysical bursts of widely varying intensity. No correlations were found between neutrinos detected in SNO and such astrophysical sources.

  15. Size and shape controllable preparation of graphene sponge by freezing, lyophilizing and reducing in container

    Institute of Scientific and Technical Information of China (English)

    ZHAO LianQin; YU BaoWei; ZHANG XiaoLiang; WU RuiHan; LIU XiaoYang; LIAO Rong; YANG ShengTao

    2016-01-01

    Graphene sponge (GS) is a porous 3D structure of graphene.Although hydrothermal reduction,chemical vapor deposition,solution reduction and high temperature annealing could be used for the preparation of GS,the size and shape cannot be well controlled.Herein,we reported a facile method to prepare GS under mild condition in a size and shape controllable way.Graphene oxide was lyophilized to form the spongy structure and reduced by steamy hydrazine hydrate to produce GS.The size and shape of GS prepared were nearly identical to that of the container.The reduction degree of GS could be regulated by the reduction temperature and time.

  16. Panchromatic Ru (II) Dipyrrins as NCS Free Sensitizers Showing Highest Efficiency for DSSCs

    International Nuclear Information System (INIS)

    Two new Ru(II) complexes bearing with substituted dipyrrinato ligands (GS3 and GS10) have been successfully synthesized and characterized. These sensitizers exhibit panchromatic light harvesting properties, rendering them promising for DSSC applications. GS3 has shown high overall power conversion efficiency (η = 2.79%) compared to GS10 which is the highest so far in the case of Ru (II) dipyrrinato dyes. Density functional theory (DFT) and time-dependent DFT calculations were measured for GS3 and GS10 to understand their structural, electronic and photophysical properties

  17. Griscelli syndrome type 2: A rare and fatal syndrome in a South Indian boy

    Directory of Open Access Journals (Sweden)

    R Rajyalakshmi

    2016-01-01

    Full Text Available Griscelli syndrome (GS is a rare autosomal recessive disorder caused by mutation in the MYO5A (GS1, RAB27A (GS2, and MLPH (GS3 genes, characterized by a common feature, partial albinism. The common variant of three, GS type 2, in addition, shows primary immunodeficiency which leads to recurrent infections and hemophagocytic lymphohistiocytosis. We, herewith, describe a case of GS type 2, in a 4-year-old male child who presented with chronic and recurrent fever, lymphadenopathy, hepatosplenomegaly, and secondary neurological deterioration; highlighting the cytological and histopathological features of lymph nodes. Hair shaft examination of the child confirmed the diagnosis.

  18. Griscelli syndrome type 2: A rare and fatal syndrome in a South Indian boy.

    Science.gov (United States)

    Rajyalakshmi, R; Chakrapani, R N B

    2016-01-01

    Griscelli syndrome (GS) is a rare autosomal recessive disorder caused by mutation in the MYO5A (GS1), RAB27A (GS2), and MLPH (GS3) genes, characterized by a common feature, partial albinism. The common variant of three, GS type 2, in addition, shows primary immunodeficiency which leads to recurrent infections and hemophagocytic lymphohistiocytosis. We, herewith, describe a case of GS type 2, in a 4-year-old male child who presented with chronic and recurrent fever, lymphadenopathy, hepatosplenomegaly, and secondary neurological deterioration; highlighting the cytological and histopathological features of lymph nodes. Hair shaft examination of the child confirmed the diagnosis. PMID:26960655

  19. Antonio Maria Baggio (ed.), Adriana Cossedu, Paolo Giusta, Rodrigo Mardones Z. y Antonio Márquez Prieto, Caino e i suoi fratelli. Il fondamento relazionale nella politica e nel diritto, Città Nuova, Roma, 2012, 247 págs.

    OpenAIRE

    María Salas Porras

    2013-01-01

    Los estudios recogidos en este volumen intentan reinterpretar los fundamentos de la Política y del Derecho, enmarcados en el horizonte relacional que la fraternidad les abre. Desde esta perspectiva, la fraternidad asume una relevancia política y jurídica en la medida en que se la entienda sustancialmente ligada a la libertad y a la igualdad. Cada una de las tres categorías, en la medida en que sean consideradas conjuntamente, llegan a adquirir su propio significado a través de la relación con...

  20. Antonio Maria Baggio (ed., Adriana Cossedu, Paolo Giusta, Rodrigo Mardones Z. y Antonio Márquez Prieto, Caino e i suoi fratelli. Il fondamento relazionale nella politica e nel diritto, Città Nuova, Roma, 2012, 247 págs.

    Directory of Open Access Journals (Sweden)

    María Salas Porras

    2013-03-01

    Full Text Available Los estudios recogidos en este volumen intentan reinterpretar los fundamentos de la Política y del Derecho, enmarcados en el horizonte relacional que la fraternidad les abre. Desde esta perspectiva, la fraternidad asume una relevancia política y jurídica en la medida en que se la entienda sustancialmente ligada a la libertad y a la igualdad. Cada una de las tres categorías, en la medida en que sean consideradas conjuntamente, llegan a adquirir su propio significado a través de la relación con las otras. El redescubrimiento de la fraternidad significa por tanto volver a proponer el "tríptico" entero, para recuperar, a través del mismo, el proyecto humanísitico de la modernidad, y liberarnos del destino del conflicto radical al que nuestro tiempo parece condenado: Caín no sólo ha matado a su hermano, sino que también ha fundado la primera ciudad, es decir, el lugar donde la violencia se ve confinada por el gobierno y por las leyes. Abrir el Derecho al horizonte de la fraternidad significa, hoy, llevar la racionalidad jurídica más allá de la mera racionalidad técnica; permitir renovar la relación jurídica, volver a encontrar sus fundamentos humanísticos, redescubriendo una justicia que reconcilia y reconstruye, fortificando el vínculo social. Considerar la fraternidad una categoría política significa poder emitir juicios políticos, capaces de producir un pensamiento político a través de la óptica de la fraternidad; se abandonan así las lógicas de la ideología, para intentar construir una forma de pensamiento en disposición de pasar, de una idea intuitiva de fraternidad, a una definición que la haga operativa, "operacionalizable". Es una tarea que no corresponde hoy a una sola disciplina, sino que interesa a todo el conjunto de los ya numerosos estudiosos que se han enrolado en este frente, interactuando entre sí de manera interdisiciplinar y abriendo un camino en el cual resulte posible, a todos los que han abrazado un humanismo y un ideal político, encontrar las mejores razones de su esperanza.

  1. Alejandro Simonoff. Teorías en movimiento. Los orígenes disciplinares de la política exterior y sus interpretaciones históricas : Rosario, Prohistoria Ediciones, 147 págs., ISBN 978-987-1855-10-0

    OpenAIRE

    Lorenzini, María Elena

    2012-01-01

    El libro Teorías en movimiento hace honor a su título puesto que, al recorrer el camino propuesto por el doctor Alejandro Simonoff, el lector percibe la evolución del proceso a través del cual se fue configurando el campo de la política exterior en Argentina; las ideas, los conceptos y las teorías elaboradas para comprender y analizar el derrotero de la misma; las mutaciones y desplazamientos que tales corrientes experimentaron en la línea del tiempo. La hoja de ruta que guía la obra se inici...

  2. Lexikon der Romanistischen Linguistik (LRL). Edité par Günter Holtus -Michael Metzeltin - Christian Schmitt. Volume VI,l. Aragones/Navarro, Español, Asturiano/Leonés. Max Niemeyer Verlag, Tübingen 1992, págs. XXXVIII+708

    OpenAIRE

    Mitja Skubic

    1993-01-01

    El Diccionario enciclopédico de lingüistica románica (LRL) es un compendio general de todo el saber romanistico sobre Ia génesis, el desarrollo y el estado actual de las lenguas romanicas. La obra consta de ocho volúmenes, de los cuales han aparecido, hasta ahora, los que tratan de las lenguas románicas desde el Renacimiento basta nuestros dias, con excepción del volumen reservado al gallego y portugués. Esperamos, por tanto, con gran interes la aparición del segundo volumen, dedicado al latí...

  3. Lexikon der Romanistischen Linguistik (LRL. Edité par Günter Holtus -Michael Metzeltin - Christian Schmitt. Volume VI,l. Aragones/Navarro, Español, Asturiano/Leonés. Max Niemeyer Verlag, Tübingen 1992, págs. XXXVIII+708

    Directory of Open Access Journals (Sweden)

    Mitja Skubic

    1993-12-01

    Full Text Available El Diccionario enciclopédico de lingüistica románica (LRL es un compendio general de todo el saber romanistico sobre Ia génesis, el desarrollo y el estado actual de las lenguas romanicas. La obra consta de ocho volúmenes, de los cuales han aparecido, hasta ahora, los que tratan de las lenguas románicas desde el Renacimiento basta nuestros dias, con excepción del volumen reservado al gallego y portugués. Esperamos, por tanto, con gran interes la aparición del segundo volumen, dedicado al latín y al desarrollo de las lenguas románicas desde la Edad Media basta el Renacimiento.

  4. Borja Gómez, Jaime y Rodríguez Jiménez, Pablo. Historia de la vida privada en Colombia. Tomo I: Las fronteras difusas del siglo XVI a 1880. Bogotá: Taurus, 2011, 396 págs.

    OpenAIRE

    Didier Francisco Ríos García

    2012-01-01

    Desde mediados de los ochenta cuando Philippe Ariès y George Duby, sacaron a la luz un trabajo sobre la vida privada, se ha generado un nuevo movimiento historiográfico que ha recorrido el mundo, el cual no trata lo que siempre la historia había trabajado, sino que se concentraba en la vida de las vidas secretas de las personas, ya fueran personajes públicos o desconocidas, también se hablo de la...

  5. Borja Gómez, Jaime y Rodríguez Jiménez, Pablo. Historia de la vida privada en Colombia. Tomo I: Las fronteras difusas del siglo XVI a 1880. Bogotá: Taurus, 2011, 396 págs.

    Directory of Open Access Journals (Sweden)

    Didier Francisco Ríos García

    2012-07-01

    Full Text Available Desde mediados de los ochenta cuando Philippe Ariès y George Duby, sacaron a la luz un trabajo sobre la vida privada, se ha generado un nuevo movimiento historiográfico que ha recorrido el mundo, el cual no trata lo que siempre la historia había trabajado, sino que se concentraba en la vida de las vidas secretas de las personas, ya fueran personajes públicos o desconocidas, también se hablo de las situaciones que vivían los sectores marginales de la población, todos visto desde el punto de vista de su vida en los ambientes alejados del escrutinio público, desde su forma de vestir, sus amores, sus aventuras, su alimentación y de las mismos espacios que frecuentaban.

  6. André Thibault, Perfecto simple y perfecto compuesto en español preclásico. Estudio de los perfectos de indicativo en "La Celestina", el "Teatro" de Encina y el "Dialogo de Ia lengua". Beihefte zur ZRPh, Band 301, Max Niemeyer Verlag, Tübingen 2000; págs.

    OpenAIRE

    Mitja Skubic

    2001-01-01

    La suerte del perfectum latino en lenguas románicas no deja de atraer a los investigadores de los problemas sintácticos. Suenan como profecía las palabras con las cuales el Autor concluyó su trabajo diciendo que en este campo queda todavía mucho por hacer; la profecía parece válida no solo para el español, sino para todo el mundo romance. Prof. André Thibault ha restringido su investigación sobre el pretérito simple y compuesto (permítaseme la terminologia a la cual estoy acostumbrado) a los ...

  7. Gustave Le Bon, Psicología de las multitudes, trad. española de J. M. Navarro de Palencia, revisión J. L. Monereo Pérez, edición y estudio preliminar de J. L. Monereo Pérez, Granada, Comares, 2012, LXXXIX+136 págs.

    OpenAIRE

    Patricia Zambrana Moral

    2012-01-01

    Nos encontramos ante una nueva edición de la Psicología de las multitudes de Gustave Le Bon, precedida de un estudio preliminar del catedrático de Derecho del Trabajo y de la Seguridad Social de la Universidad de Granada José Luis Monereo Péreztitulado ««La era de las masas»: el pensamiento socio-político de Gustave Le Bon«. Monereo considera a Le Bon «uno de los fundadores principales de la psicología social». Refleja su influjo en diversas orientaciones ideológicas contrapuestas y examina l...

  8. André Thibault, Perfecto simple y perfecto compuesto en español preclásico. Estudio de los perfectos de indicativo en "La Celestina", el "Teatro" de Encina y el "Dialogo de Ia lengua". Beihefte zur ZRPh, Band 301, Max Niemeyer Verlag, Tübingen 2000; págs.

    Directory of Open Access Journals (Sweden)

    Mitja Skubic

    2001-12-01

    Full Text Available La suerte del perfectum latino en lenguas románicas no deja de atraer a los investigadores de los problemas sintácticos. Suenan como profecía las palabras con las cuales el Autor concluyó su trabajo diciendo que en este campo queda todavía mucho por hacer; la profecía parece válida no solo para el español, sino para todo el mundo romance. Prof. André Thibault ha restringido su investigación sobre el pretérito simple y compuesto (permítaseme la terminologia a la cual estoy acostumbrado a los tres grandes acontecimientos en la literatura española, indicados en el título, pero antepuso a su trabajo un amplio panorama histórico, incluyendo en ello anotaciones sobre las tendencias en algunos idiomas indoeuropeos que demuestran los usos de las formas simples y las compuestas y no sólo en el campo del pretérito.

  9. Felipe González Vicén, Teoría de la revolución. Sistema e historia, Consejo Superior de Investigaciones Científicas y Plaza y Valdés, Madrid y México, D. F., 2010, 125 págs.

    Directory of Open Access Journals (Sweden)

    Manuel J. Peláez

    2013-03-01

    Full Text Available Nos encontramos ante la reedición de un libro clásico de Felipe González Vicén, catedrático de Derecho Natural y Filosofía del Derecho que lo fue de las Universidades de Sevilla y La Laguna, fallecido en 1991. Su tesis doctoral, que indudablemente está en este libro, fue menospreciada por el tribunal llamado a juzgarla en la Universidad de Madrid, otorgándole la calificación de Aprobado. La figura de González Vicén está acompañada de una leyenda, que conviene ir aclarando poco a poco con el paso del tiempo. Autor de fina pluma y con un gran conocimiento de la doctrina alemana, recoge en el presente libro la evolución de las teorías sobre la revolución, incidiendo en la importancia que tiene el orden jurídico establecido y el derecho de resistencia ante la existencia de poderes injustos. También determina la diferenciación conceptual que puede hacerse entre revolución, golpe de Estado, conquista y reformas de las constituciones vigentes.

  10. 77 FR 32146 - Excepted Service

    Science.gov (United States)

    2012-05-31

    .... This authority may be used to fill GS-080 (Security) and GS-132 (Intelligence) positions. No... Congressional Affairs. Office of Senior Counsel..... DB120055 2/24/2012 Innovation and Improvement. Office...

  11. ACTIVITIES OF AMMONIA ASSIMILATION ENZYMES AS INDICATORS OF THE RELATIVE SUPPLY OF NITROGEN SUBSTRATES FOR MARINE BACTERIOPLANKTON IN SUB-TROPICAL COASTAL WATER

    Science.gov (United States)

    The supply of nitrogen substrates available for bacterial production in seawater was determined using the activities of ammonia assimilation enzymes, glutamine synthetase (GS) and glutamate dehydrogenase (GDH). Expression of GS and GDH by bacteria in pure culture is generally ind...

  12. Seasonal variations in physicochemical profiles of Guduchi Satva (starchy substance from Tinospora cordifolia [Willd.] Miers)

    OpenAIRE

    Rohit Sharma; Hetal Amin; Galib R; P K Prajapati

    2013-01-01

    Guduchi Satva (GS), the starchy material extracted from the Guduchi stem is well-known Ayurvedic single drug formulation having a wide range of therapeutic utility. Species of the plant, stem size, collection time, season and maturity of the plant may affect the yield and physico-chemical profile of GS. However, published data on such variations is lacking. Considering this, present study is planned to screen seasonal variations in physico-chemical profile of GS. 18 batches of GS were prepare...

  13. The glutamine synthetase gene family in Populus

    OpenAIRE

    Cánovas Francisco M; Kirby Edward G; Avila Concepción; Canales Javier; García-Gutiérrez Angel; Castro-Rodríguez Vanessa

    2011-01-01

    Abstract Background Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is a key enzyme in ammonium assimilation and metabolism of higher plants. The current work was undertaken to develop a more comprehensive understanding of molecular and biochemical features of GS gene family in poplar, and to characterize the developmental regulation of GS expression in various tissues and at various times during the poplar perennial growth. Results The GS gene family consists ...

  14. Identification of Intact High Molecular Weight Glutenin Subunits from the Wheat Proteome Using Combined Liquid Chromatography-Electrospray Ionization Mass Spectrometry

    OpenAIRE

    Lagrain, Bert; Brunnbauer, Markus; Rombouts, Ine; Koehler, Peter

    2013-01-01

    The present paper describes a method for the identification of intact high molecular weight glutenin subunits (HMW-GS), the quality determining proteins from the wheat storage proteome. The method includes isolation of HMW-GS from wheat flour, further separation of HMW-GS by reversed-phase high-performance liquid chromatography (RP-HPLC), and their subsequent molecular identification with electrospray ionization mass spectrometry using a quadrupole-time-of-flight mass analyzer. For HMW-GS iso...

  15. Gender-Segregated Schooling: A Problem Disguised as a Solution

    Science.gov (United States)

    Fabes, Richard A.; Martin, Carol Lynn; Hanish, Laura D.; Galligan, Kathrine; Pahlke, Erin

    2015-01-01

    Gender-segregated (GS) schooling has become popular in the United States despite the fact that every major review has concluded that GS schooling is not superior to coeducational schooling. Moreover, concern has been raised that GS schooling leads to negative effects, including increased gender stereotyping. We argue that these negative effects…

  16. Dicty_cDB: Contig-U16566-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _GS-35-01-01-1... 64 3e-27 3 ( EJ044277 ) 1095454111632 Global-Ocean-Sampling_GS-...male Pupae Nasoni... 105 8e-21 2 ( EJ805472 ) 1093017455782 Global-Ocean-Sampling_GS-30-02-01-1... 68 8e-21 3 ( EK141129 ) 1095454111

  17. An Appreciative Inquiry Exploring Game Sense Teaching in Physical Education

    Science.gov (United States)

    Pill, Shane

    2016-01-01

    This paper reports on research framed as a strengths-based appreciative inquiry (AI) into the use of a game sense (GS) approach for sport and games teaching in physical education (PE). The aim of this research was to find the elements which sustain teachers in the use of a GS approach. This is particularly pertinent given strong advocacy for GS as…

  18. 5 CFR 9901.372 - Conversion or movement out of NSPS pay system.

    Science.gov (United States)

    2010-01-01

    ....) If an employee's adjusted salary is less than the minimum rate of the highest applicable GS rate... using GS pay retention rules in 5 CFR part 536 (if the employee is eligible for pay retention under... to a GS position under this section to the extent provided under 5 CFR part 531, subpart D....

  19. Cellular and subcellular localization of flavin-monooxygenases involved in glucosinolate biosynthesis

    DEFF Research Database (Denmark)

    Li, Jing; Kristiansen, Kim A.; Hansen, Bjarne Gram; Halkier, Barbara Ann

    2011-01-01

    compartmentation of FMO(GS-OX1) was also detected by transiently expressing a FMO(GS-OX1)-yellow fluorescent protein (YFP) fusion protein in tobacco leaves. The results showed that FMO(GS-OX1-5) were expressed basically in vascular tissues, especially in phloem cells, like other glucosinolate biosynthetic genes...

  20. Enzymology and Molecular Biology of Cell Wall Biosynthesis. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Peter M. Ray

    2000-04-01

    The following aspects of enzymology of cell wall synthesis were pursued under this cited grant: (1) Isolation of plasma membrane-localized glucan synthase II (GS-II) of pea; (2) Cloning of genes for possible plant GS-II components; (3) Golgi glucan synthase-I (GS-I); and (4) Golgi reversibly glycosylated protein 1 (RGP1).

  1. The glutamine synthetase gene family in Populus

    Directory of Open Access Journals (Sweden)

    Cánovas Francisco M

    2011-08-01

    Full Text Available Abstract Background Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming is a key enzyme in ammonium assimilation and metabolism of higher plants. The current work was undertaken to develop a more comprehensive understanding of molecular and biochemical features of GS gene family in poplar, and to characterize the developmental regulation of GS expression in various tissues and at various times during the poplar perennial growth. Results The GS gene family consists of 8 different genes exhibiting all structural and regulatory elements consistent with their roles as functional genes. Our results indicate that the family members are organized in 4 groups of duplicated genes, 3 of which code for cytosolic GS isoforms (GS1 and 1 which codes for the choroplastic GS isoform (GS2. Our analysis shows that Populus trichocarpa is the first plant species in which it was observed the complete GS family duplicated. Detailed expression analyses have revealed specific spatial and seasonal patterns of GS expression in poplar. These data provide insights into the metabolic function of GS isoforms in poplar and pave the way for future functional studies. Conclusions Our data suggest that GS duplicates could have been retained in order to increase the amount of enzyme in a particular cell type. This possibility could contribute to the homeostasis of nitrogen metabolism in functions associated to changes in glutamine-derived metabolic products. The presence of duplicated GS genes in poplar could also contribute to diversification of the enzymatic properties for a particular GS isoform through the assembly of GS polypeptides into homo oligomeric and/or hetero oligomeric holoenzymes in specific cell types.

  2. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)

    DEFF Research Database (Denmark)

    Bernard, Stéphanie M.; Møller, Anders Laurell Blom; Dionisio, Giuseppe;

    2008-01-01

    ). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular...... sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle...

  3. Fabrication and evaluation of TiO2/graphite silica bilayer thin films prepared by laser ablation method

    International Nuclear Information System (INIS)

    The bilayer thin film composed of graphite silica (GS) and TiO2 was prepared by the laser ablation technique. The properties of the thin films considerably depended on the laser ablation conditions such as gas pressure and substrate temperature in a chamber. The laser ablation of GS caused complete disappearance of the quartz XRD (X-ray diffraction) peaks in GS. The GS loading, especially at a thickness of around 200 nm, led to significant enhancement in the photocatalytic activity of the TiO2 thin film for the hydrogen production and the methylene blue decomposition in spite of the destruction of the quartz crystal in GS

  4. Fabrication and evaluation of TiO{sub 2}/graphite silica bilayer thin films prepared by laser ablation method

    Energy Technology Data Exchange (ETDEWEB)

    Somekawa, Shouichi [Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065 (Japan); Kusumoto, Yoshihumi [Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065 (Japan)], E-mail: kusumoto@sci.kagoshima-u.ac.jp; Ikeda, Miyuki; Ngweniform, Pascaline [Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065 (Japan)

    2008-11-28

    The bilayer thin film composed of graphite silica (GS) and TiO{sub 2} was prepared by the laser ablation technique. The properties of the thin films considerably depended on the laser ablation conditions such as gas pressure and substrate temperature in a chamber. The laser ablation of GS caused complete disappearance of the quartz XRD (X-ray diffraction) peaks in GS. The GS loading, especially at a thickness of around 200 nm, led to significant enhancement in the photocatalytic activity of the TiO{sub 2} thin film for the hydrogen production and the methylene blue decomposition in spite of the destruction of the quartz crystal in GS.

  5. La inútil añoranza de la normalidad

    Directory of Open Access Journals (Sweden)

    Margarita Valencia

    2014-02-01

    Full Text Available Litchis de Madagascar. Aquiles Cuervo. Editorial Elfin de la noche. Buenos Aires, 2011, 91 págs. El ruido de las cosas al caer. Juan Gabriel Vásquez. Premio Alfaguara de novela 2011. Alfaguara. Bogotá. 2011, 259 págs. Tres ataúdes blancos. Antonio Ungar. Premio Herralde de Novela. Editorial Anagrama. Barcelona. 2010, 259 págs. Suicídame. Andrés Arias. Ediciones B. Bogotá, 2010, 261 págs. C.M. no récord. Juan Álvarez. Alfaguara. Bogotá, 2010, 262 págs.

  6. Influence of nutrients, disease, and air pollution on glucosinolates in cruciferous vegetables

    Energy Technology Data Exchange (ETDEWEB)

    Wang Weiming.

    1993-09-01

    The influence of three environmental factors, nitrogen and sulfur nutrition, nitrogen dioxide fumigation, and turnip mosaic virus (TuMv) infection, on the glucosinolate (GS) content and profile in pakchoy (Brassic campestris ssp chinensis) and kale (Brassica oleracea) plants was investigated. An interaction between N and S nutrition on the GA concentration in pakchoy and kale plants was detected. The GS concentration in pakchoy was elevated and the plant growth was retarded at low N and S treatments. Increasing N resulted in a decrease in GS and increase in plant growth. High N and S treatment induced toxicity symptoms in kale and increased GS concentration. N and S treatment altered the GS distribution among different leaves. NO[sub 2] fumigation altered GS concentration in pakchoy plants. In both seedlings and mature plants, the GS increased when plants were exposed to increasing NO[sub 2] levels. TuMv infection altered the GS concentrations in pakchoy. Early infected plants suffered greater growth reduction and alteration in total GS and several individual GS. N/S nutrition, NO[sub 2] fumigation and TuMv infection had no effect on GS profile. 172 refs., 22 figs., 21 tabs.

  7. Overexpression of glutamine synthetases confers transgenic rice herbicide resistance

    Institute of Scientific and Technical Information of China (English)

    Sun Hui; Huang Qiman; Su Jin

    2005-01-01

    Glutamine synthetase (GS, E.C.6.3.1.2) is a key enzyme involved in the assimilation of inorganic nitrogen in higher plants and gram-negative microorganisms. GS is the targeting enzyme of a herbicide phosphinothricin (PPT) or Basta. In order to generate PPT-resistant transgenic rice via overexpression of GS, we constructed a plant expression vector p2GS harboring two different isoenzymes GS1 and GS2 cDNAs under the control of constitutive promoters of rice Act1 and maize Ubiquitin(Ubi) genes. The p2GS was introduced into rice genome by Agrobacterium-mediated transformation and confirmed by PCR and Southern blot hybridization. GS-transgene expression was first detected by Northern blot analyses. Results from Basta test indicated that GS-transgenic plants can tolerate as high as 0.3% Basta solution. In addition, our results also demonstrated that GS overexpression conferred transformed rice calli PPT resistance. Thus, GS cassette can serve as a selective marker gene instead of bar cassette for selection of PPT transformants.

  8. Graphene sheets stacked polyacrylate latex composites for ultra-efficient electromagnetic shielding

    Science.gov (United States)

    Li, Yong; Zhang, Song; Ni, Yuwei

    2016-07-01

    Graphene sheets (GS) are at the forefront of electromagnetic interference (EMI) shielding/attenuation materials science research because of their excellent electrical properties (Wen B et al 2014 Adv. Mater. 26 3484, Zhang Y et al 2015 Adv. Mater. 27 2049). GS/polyacrylate (PA) composites were prepared using a solvent-free latex technology, which favored the build-up of a segregated GS architecture stacked in the polymer matrix. GS were obtained from graphite flakes (GF) via a mechanical delamination approach in water. The microstructure, electrical, dielectric and electromagnetic shielding properties of the GS/PA composites were correlated in this manuscript. A remarkably low percolation threshold of ∼0.11 mass per cent for room-temperature electrical conductivity was obtained in the GS/PA composites owing to the stacked architecture of GS with high aspect ratios. This unique nanostructured GS architecture not only enhanced the electrical conductivity of composites, but also dramatically increased complex permittivity by inducing strong Maxwell–Wagner–Sillars (MWS) polarization at the highly conductive GS/non-conductive PA interfaces. The EMI shielding effectiveness (SE) of these composites was enhanced with increasing GS content, and the composite with 6 wt% GS loading exhibited a high EMI SE of ∼66 dB over a frequency of 8.2–12.4 GHz, resulting from the pronounced conduction loss, dielectric relaxation, and multi-scattering.

  9. Tissue-specific changes of glutamine synthetase activity in oats after rhizosphere infestation by Pseudomonas syringae pv. tabaci. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Knight, T.J. [Univ. of Southern Maine, Portland, ME (United States); Temple, S.; Sengupta-Gopalan, C. [New Mexico State Univ., Las Curces, NM (United States)] [and others

    1996-05-15

    Oats (Avena sativa L. lodi) tolerant of rhizosphere infestation by Pseudomonas syringae pv. tabaci when challenged by the pathogen experience tissue-specific alterations of ammonia assimilatory capabilities. Altered ammonia assimilatory potentials between root and leaf tissue result from selective inactivation of glutamine synthetase (GS) by the toxin Tabtoxinine-B-lactam (TBL). Root GS is sensitive and leaf GSs are resistant to TBL inactivation. With prolonged challenge by the pathogen root GS activity decreases but leaf GS specific activity increase. Higher leaf GS activity is due to decreased rates of degradation rather than increased GS synthesis. Higher leaf GS activity and elevated levels of GS polypeptide appear to result from a limited interaction between GS and TBL leading to the accumulation of a less active but more stable GS holoenzyme. Tolerant challenged oats besides surviving rhizosphere infestation, experience enhanced growth. A strong correlation exists between leaf GS activity and whole plant fresh weight, suggesting that tissue-specific changes in ammonia assimilatory capability provides the plant a more efficient mechanism for uptake and utilization of nitrogen.

  10. Porous magnesium loaded with gentamicin sulphate and in vitro release behavior.

    Science.gov (United States)

    Li, Qiuyan; Jiang, Guofeng; Wang, Dong; Wang, Huang; Ding, Liang; He, Guo

    2016-12-01

    Our aim was to develop a biocompatible bone repair material that has the advantage of preventing postoperative infections. Finally, the porous magnesium (p-Mg) loaded with gentamicin sulphate (GS-loaded Mg-G) was fabricated. The GS release behavior of the GS-loaded Mg-G in phosphate buffer saline (PBS) was investigated. The effective release time of GS reached to 14days. In addition, the effects of porosity and pore diameter of p-Mg on the GS release behavior of the GS-loaded Mg-G were studied. In the initial burst release stage, the GS release rate of the GS-loaded Mg-G increased with the increasing porosity or the increasing pore diameter of p-Mg. The GS-loaded Mg-G with larger original pore diameter has higher burst release of GS. Moreover, the in vitro antibacterial test of the GS-loaded Mg-G indicated that this biomaterial has obvious antibacterial effect. This study can provide information for p-Mg loaded with drug(s) as functional bone repair materials with drug-delivery capabilities. PMID:27612700

  11. One-pot hydrothermal preparation of graphene sponge for the removal of oils and organic solvents

    Science.gov (United States)

    Wu, Ruihan; Yu, Baowei; Liu, Xiaoyang; Li, Hongliang; Wang, Weixuan; Chen, Lingyun; Bai, Yitong; Ming, Zhu; Yang, Sheng-Tao

    2016-01-01

    Graphene sponge (GS) has found applications in oil removal due to the hydrophobic nature of graphene sheets. Current hydrothermal preparations of GS use toxic reducing reagents, which might cause environmental pollution. In this study, we reported that graphene oxide (GO) could be hydrothermally reduced by glucose to form GS for the adsorption of oils and various organic solvents. Graphene sheets were reduced by glucose during the hydrothermal treatment and formed 3D porous structure. GS efficiently adsorbed organic solvents and oils with competitive adsorption capacities. GS was able to treat pollutants in pure liquid form and also in the simulated seawater. GS could be easily regenerated by evaporating or burning. After 10 cycles, the adsorption capacity still retained 77% by evaporating and 87% by burning. The implication to the applications of GS in water remediation is discussed.

  12. Glutamine Synthetase in Legumes: Recent Advances in Enzyme Structure and Functional Genomics

    Directory of Open Access Journals (Sweden)

    Marco Betti

    2012-06-01

    Full Text Available Glutamine synthetase (GS is the key enzyme involved in the assimilation of ammonia derived either from nitrate reduction, N2 fixation, photorespiration or asparagine breakdown. A small gene family is encoding for different cytosolic (GS1 or plastidic (GS2 isoforms in legumes. We summarize here the recent advances carried out concerning the quaternary structure of GS, as well as the functional relationship existing between GS2 and processes such as nodulation, photorespiration and water stress, in this latter case by means of proline production. Functional genomic analysis using GS2-minus mutant reveals the key role of GS2 in the metabolic control of the plants and, more particularly, in carbon metabolism.

  13. The Relationship between GRACE Score and Epicardial Fat Thickness in non-STEMI Patients

    Directory of Open Access Journals (Sweden)

    Ilker Gul

    2016-01-01

    Full Text Available Abstract Background: GRACE risk score (GS is a scoring system which has a prognostic significance in patients with non-ST segment elevation myocardial infarction (non-STEMI. Objective: The present study aimed to determine whether end-systolic or end-diastolic epicardial fat thickness (EFT is more closely associated with high-risk non-STEMI patients according to the GS. Methods: We evaluated 207 patients who had non-STEMI beginning from October 2012 to February 2013, and 162 of them were included in the study (115 males, mean age: 66.6 ± 12.8 years. End-systolic and end-diastolic EFTs were measured with echocardiographic methods. Patients with high in-hospital GS were categorized as the H-GS group (in hospital GS > 140, while other patients were categorized as the low-to-moderate risk group (LM-GS. Results: Systolic and diastolic blood pressures of H-GS patients were lower than those of LM-GS patients, and the average heart rate was higher in this group. End-systolic EFT and end-diastolic EFT were significantly higher in the H-GS group. The echocardiographic assessment of right and left ventricles showed significantly decreased ejection fraction in both ventricles in the H-GS group. The highest correlation was found between GS and end-diastolic EFT (r = 0.438. Conclusion: End-systolic and end-diastolic EFTs were found to be increased in the H-GS group. However, end-diastolic EFT and GS had better correlation than end-systolic EFT and GS.

  14. Identifying crucial parameter correlations maintaining bursting activity.

    Directory of Open Access Journals (Sweden)

    Anca Doloc-Mihu

    2014-06-01

    Full Text Available Recent experimental and computational studies suggest that linearly correlated sets of parameters (intrinsic and synaptic properties of neurons allow central pattern-generating networks to produce and maintain their rhythmic activity regardless of changing internal and external conditions. To determine the role of correlated conductances in the robust maintenance of functional bursting activity, we used our existing database of half-center oscillator (HCO model instances of the leech heartbeat CPG. From the database, we identified functional activity groups of burster (isolated neuron and half-center oscillator model instances and realistic subgroups of each that showed burst characteristics (principally period and spike frequency similar to the animal. To find linear correlations among the conductance parameters maintaining functional leech bursting activity, we applied Principal Component Analysis (PCA to each of these four groups. PCA identified a set of three maximal conductances (leak current, [Formula: see text]Leak; a persistent K current, [Formula: see text]K2; and of a persistent Na+ current, [Formula: see text]P that correlate linearly for the two groups of burster instances but not for the HCO groups. Visualizations of HCO instances in a reduced space suggested that there might be non-linear relationships between these parameters for these instances. Experimental studies have shown that period is a key attribute influenced by modulatory inputs and temperature variations in heart interneurons. Thus, we explored the sensitivity of period to changes in maximal conductances of [Formula: see text]Leak, [Formula: see text]K2, and [Formula: see text]P, and we found that for our realistic bursters the effect of these parameters on period could not be assessed because when varied individually bursting activity was not maintained.

  15. Prognostic Discrimination Using a 70-Gene Signature among Patients with Estrogen Receptor-Positive Breast Cancer and an Intermediate 21-Gene Recurrence Score

    Directory of Open Access Journals (Sweden)

    Sung Gwe Ahn

    2013-12-01

    Full Text Available The Oncotype DX® recurrence score (RS predictor has been clinically utilized to appropriately select adjuvant chemotherapy for patients with estrogen receptor (ER-positive early breast cancer. However, the selection of chemotherapy for patients with intermediate RSs remains controversial. We assessed the prognostic value of a 70-gene signature (70GS among patients with ER-positive breast cancer and intermediate RSs. In addition, we sought to identify genes associated with poor 70GS scores based on gene expression profiling (GEP. GEP was performed using gene expression data from 186 patients with ER-positive breast cancer. The RS and 70GS score were calculated on the basis of GEP. Among 186 patients, 82 ER-positive patients with intermediate RSs were identified. These patients were stratified by 70GS, overall survival (OS significantly differed according to 70GS (p = 0.013. In a supervised hierarchical analysis according to 70GS, the expression of several representative genes for cell proliferation was significantly higher in the poor 70GS cluster than in the good 70GS cluster. Furthermore, among these patients, FOXM1, AURKA, AURKB, and BIRC5 displayed prognostic significance for OS. In conclusion, 70GS can help to discriminate survival differences among ER-positive patients with intermediate RSs. FOXM1, AURKA, AURKB, and BIRC5, are associated with poor 70GS scores.

  16. Molecular imaging of cholinergic processes in prostate cancer using {sup 11}C-donepezil and {sup 18}F-FEOBV

    Energy Technology Data Exchange (ETDEWEB)

    Stokholm, Morten Gersel; Bender, Dirk; Jakobsen, Steen; Froekiaer, Joergen; Borghammer, Per [Aarhus University Hospital, Department of Nuclear Medicine and PET Centre, Aarhus C (Denmark); Hoeyer, Soeren [Aarhus University Hospital, Department of Histopathology, Aarhus C (Denmark); Borre, Michael [Aarhus University Hospital, Department of Urology, Aarhus C (Denmark)

    2016-05-15

    High-grade prostate cancer (PC) displays parasympathetic neoneurogenesis. We investigated the binding of two PET tracers that visualize cholinergic nerves in PC tissue using autoradiography. Prostatectomy tissue was subjected to autoradiography with {sup 11}C-donepezil and {sup 18}F-FEOBV and correlated with Gleason scores (GS). Regions of interest on the autoradiograms were defined and quantified. Tracer binding in cancer tissue regions was compared with that in normal tissue. We included 13 patients with biopsy-verified PC. In particular, {sup 11}C-donepezil uptake was higher in ''high-grade'' PC (GS ≥4 + 3) than in ''low-grade'' PC and benign hyperplasia. {sup 11}C-donepezil uptake ranged from a mean of 56 % higher (GS 3 + 3) to 409 % higher (GS 4 + 4), and {sup 18}F-FEOBV uptake ranged from 67 % higher (GS 3 + 3) to 194 % higher (GS 4 + 5). The uptake of both tracers was higher in PC with a high GS than in PC with a low GS, but the difference was significant only for {sup 11}C-donepezil (p = 0.003). Uptake of PET tracers binding to cholinergic nerves was markedly higher in PC with a high GS than in PC with a low GS. This finding implies that {sup 11}C-donepezil PET/CT may be able to differentiate between low-grade and high-grade PC. (orig.)

  17. Epigenetic modifications and self-renewal regulation of mouse germline stem cells

    Institute of Scientific and Technical Information of China (English)

    Jiyoung Lee; Takashi Shinohara

    2011-01-01

    Germline stem (GS) cells were established from gonocytes and spermatogonia of postnatal mouse testes. GS cells proliferate in the presence of several kinds of cytokines, and a small percentage of GS cells also show spermatogonial stem cell (SSC) activity, i.e., they differentiate into sperm after being transplanted into infertile mouse testes without endogenous spermatogenesis. Interestingly, in GS cell culture, we also found that pluripotent stem cells (multipotent germline stem cells (mGS cells)) could be derived and these mGS cells do not have normal androgenetic genomic imprinting marks that are shown in GS cells, e.g., H19 hypermethylation. A new culture system for fetal male germ cells (embryonic GS (eGS) cells) has also been recently developed. Although these cells exhibited SSC potential, the offspring from cultured cells showed heritable imprinting defects in their DNA methylation patterns. In an attempt to understand the self-renewal machinery in SSCs, we transfected H-Ras and cylin D2 into GS cells, and successfully reconstructed the SSC self-renewal ability without using exogenous cytokines. Although these cells showed SSC activity in germ cell transplantation assays, we also found development of seminomatous tumors, possibly induced by excessive self-renewing signal. These stem cell culture systems are useful tools not only for understanding the mechanisms of self-renewal or epigenetic reprogramming but also for clarifying the mechanism of germ cell tumor development.

  18. Molecular imaging of cholinergic processes in prostate cancer using 11C-donepezil and 18F-FEOBV

    International Nuclear Information System (INIS)

    High-grade prostate cancer (PC) displays parasympathetic neoneurogenesis. We investigated the binding of two PET tracers that visualize cholinergic nerves in PC tissue using autoradiography. Prostatectomy tissue was subjected to autoradiography with 11C-donepezil and 18F-FEOBV and correlated with Gleason scores (GS). Regions of interest on the autoradiograms were defined and quantified. Tracer binding in cancer tissue regions was compared with that in normal tissue. We included 13 patients with biopsy-verified PC. In particular, 11C-donepezil uptake was higher in ''high-grade'' PC (GS ≥4 + 3) than in ''low-grade'' PC and benign hyperplasia. 11C-donepezil uptake ranged from a mean of 56 % higher (GS 3 + 3) to 409 % higher (GS 4 + 4), and 18F-FEOBV uptake ranged from 67 % higher (GS 3 + 3) to 194 % higher (GS 4 + 5). The uptake of both tracers was higher in PC with a high GS than in PC with a low GS, but the difference was significant only for 11C-donepezil (p = 0.003). Uptake of PET tracers binding to cholinergic nerves was markedly higher in PC with a high GS than in PC with a low GS. This finding implies that 11C-donepezil PET/CT may be able to differentiate between low-grade and high-grade PC. (orig.)

  19. A Taiwanese Propolis Derivative Induces Apoptosis through Inducing Endoplasmic Reticular Stress and Activating Transcription Factor-3 in Human Hepatoma Cells

    Directory of Open Access Journals (Sweden)

    Fat-Moon Suk

    2013-01-01

    Full Text Available Activating transcription factor-(ATF- 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. NBM-TP-007-GS-002 (GS-002 is a Taiwanese propolin G (PPG derivative. In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro. First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose polymerase (PARP. GS-002 also induced endoplasmic reticular (ER stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78, growth arrest- and DNA damage-inducible gene 153 (GADD153, phosphorylated eukaryotic initiation factor 2α (eIF2α, phosphorylated protein endoplasmic-reticular-resident kinase (PERK, and ATF-3. The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK signaling pathways in GS-002-treated cells. Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. These results suggest that the induction of apoptosis by the propolis derivative, GS-002, is partially mediated through ER stress and ATF-3-dependent pathways, and GS-002 has the potential for development as an antitumor drug.

  20. Long Type I X-ray Bursts and Neutron Star Interior Physics

    OpenAIRE

    Cumming, Andrew; Macbeth, Jared; Zand, J. J. M. in't; Page, Dany

    2005-01-01

    Superbursts are very energetic Type I X-ray bursts discovered in recent years by long term monitoring of X-ray bursters, believed to be due to unstable ignition of carbon in the deep ocean of the neutron star. A number of "intermediate duration" bursts have also been observed, probably associated with ignition of a thick helium layer. We investigate the sensitivity of these long X-ray bursts to the thermal profile of the neutron star crust and core. We first compare cooling models of superbur...

  1. The Thermal Evolution following a Superburst on an Accreting Neutron Star

    OpenAIRE

    Cumming, Andrew; Macbeth, Jared

    2004-01-01

    Superbursts are very energetic Type I X-ray bursts discovered in recent years by long term monitoring of X-ray bursters, and believed to be due to unstable ignition of carbon in the deep ocean of the neutron star. In this Letter, we follow the thermal evolution of the surface layers as they cool following the burst. The resulting lightcurves agree very well with observations for layer masses and energy releases in the range expected from ignition calculations. At late times, the cooling flux ...

  2. Review of low-mass X-ray binaries near the Galactic center

    OpenAIRE

    Lutovinov, A.; Grebenev, S.; Molkov, S.; Sunyaev, R.

    2003-01-01

    Results of observations of several LMXBs in the Galactic center region carried out with the ART-P telescope on board \\Granat observatory are briefly reviewed. More than dozen sources were revealed in this region during five series of observations which were performed with the ART-P telescope in 1990-1992. The investigation of the spectral evolution of persistent emission of two X-ray bursters GX3+1 and KS1731-260, discussion of QPO and spectral variations detected from the very bright Z-sourc...

  3. Results from the Antarctic Muon and Neutrino Detector Array (AMANDA)

    CERN Document Server

    Cowen, D F

    2003-01-01

    We show new results from both the older and newer incarnations of AMANDA (AMANDA-B10 and AMANDA-II, respectively). These results demonstrate that AMANDA is a functioning, multipurpose detector with significant physics and astrophysics reach. They include a new higher-statistics measurement of the atmospheric muon neutrino flux and preliminary results from searches for a variety of sources of ultrahigh energy neutrinos: generic point sources, gamma-ray bursters and diffuse sources producing muons in the detector, and diffuse sources producing electromagnetic or hadronic showers in or near the detector.

  4. New Eyes on the Universe Twelve Cosmic Mysteries and the Tools We Need to Solve Them

    CERN Document Server

    Webb, Stephen

    2012-01-01

    "New Eyes on the Universe -- Twelve Cosmic Mysteries and the Tools We Need to Solve Them" gives an up-to-date broad overview of some of the key issues in modern astronomy and cosmology. It describes the vast amount of observational data that the new generation of observatories and telescopes are currently producing, and how that data might solve some of the outstanding puzzles inherent in our emerging world view. Included are questions such as: What is causing the Universe to blow itself apart? What could be powering the luminous gamma-ray bursters? Where is all the matter in the Uni

  5. INTEGRAL monitoring of unusually long X-ray bursts

    DEFF Research Database (Denmark)

    Chenevez, Jérôme; Falanga, M.; Kuulkers, E.;

    2008-01-01

    exceptional burst events lasting more than ~10 minutes. Half of the dozen so-called intermediate long bursts registered so far have been observed by INTEGRAL. The goal is to derive a comprehensive picture of the relationship between the nuclear ignition processes and the accretion states of the system leading......X-ray bursts are thermonuclear explosions on the surface of accreting neutron stars in X-ray binaries. As most of the known X-ray bursters are frequently observed by INTEGRAL, an international collaboration have been taking advantage of its instrumentation to specifically monitor the occurrence of...

  6. X-ray Spectral Diagnostics of $\\gamma$-Ray Burst Environments

    CERN Document Server

    Paerels, F B S; Heise, J; Liedahl, D A; Paerels, Frits; Kuulkers, Erik; Heise, John; Liedahl, Duane A.

    2000-01-01

    Recently, the detection of discrete features in the X-ray afterglow spectra of GRB970508 and GRB970828 was reported. The most natural interpretation of these features is that they are redshifted Fe K emission complexes. The identification of the line emission mechanism has drastic implications for the inferred mass of radiating material, end hence the nature of the burst site. X-ray spectroscopy provides a direct observational constraint on these properties of gamma-ray bursters. We briefly discuss how these constraints arise, in the context of an application to the spectrum of GRB970508.

  7. Beasts in Lambda-CDM Zoo

    CERN Document Server

    Dolgov, A D

    2016-01-01

    Recent astronomical discoveries of supermassive black holes (quasars), gamma-bursters, supernovae, and dust at high redshifts, z = (5 --10), are reviewed. Such a dense population of the early universe is at odds with the conventional mechanisms of its possible origin. Similar data from the contemporary universe, which are also in conflict with natural expectations, are considered too. Two possible mechanisms are suggested, at least one of which can potentially solve all these problems. As a by-product of the last model, an abundant cosmological antimatter may be created.

  8. Focused study of thermonuclear bursts on neutron stars

    DEFF Research Database (Denmark)

    Chenevez, Jérôme

    X-ray bursters are a class of Low Mass X-Ray Binaries where accreted material from a donor star undergoes rapid thermonuclear burning in the surface layers of a neutron star. The flux released can temporarily exceed the Eddington limit and drive the photosphere to large radii. Such photospheric...... detection would thus probe the nuclear burning as well as the gravitational redshift from the neutron star. Moreover, likely observations of atomic X-ray spectral components reflected from the inner accretion disk have been reported. The high spectral resolution capabilities of Simbol X may therefore make...

  9. Focused study of thermonuclear bursts on neutron stars

    DEFF Research Database (Denmark)

    Chenevez, Jérôme

    2009-01-01

    X-ray bursters form a class of Low Mass X-Ray Binaries where accreted material from a donor star undergoes rapid thermonuclear burning in the surface layers of a neutron star. The flux released can temporarily exceed the Eddington limit and drive the photosphere to large radii. Such photospheric...... Simbol-X and NuSTAR. A positive detection would thus probe the nuclear burning as well as the gravitational redshift from the neutron star. Moreover, likely observations of atomic X-ray spectral components reflected from the inner accretion disk have been reported. The high spectral resolution...

  10. Long X-ray burst monitoring with INTEGRAL

    DEFF Research Database (Denmark)

    X-ray bursts are thermonuclear explosions on the surface of accreting neutron stars in low mass X-ray binary systems. In the frame of the INTEGRAL observational Key Programme over the Galactic Center a good number of the known X-ray bursters are frequently being monitored. An international....... Of special interest are low luminosity bursting sources that exhibit X-ray bursts of very different durations allowing to study the transition from a hydrogen-rich bursting regime to a pure helium regime and from helium burning to carbon burning. I will present results obtained from INTEGRAL archive...

  11. Mixed-mode synchronization between two inhibitory neurons with post-inhibitory rebound

    Science.gov (United States)

    Nagornov, Roman; Osipov, Grigory; Komarov, Maxim; Pikovsky, Arkady; Shilnikov, Andrey

    2016-07-01

    We study an array of activity rhythms generated by a half-center oscillator (HCO), represented by a pair of reciprocally coupled neurons with post-inhibitory rebounds (PIR). Such coupling-induced bursting possesses two time scales, one for fast spiking and another for slow quiescent periods, is shown to exhibit an array of synchronization properties. We discuss several HCO configurations constituted by two endogenous bursters, by tonic-spiking and quiescent neurons, as well as mixed-mode configurations composed of neurons of different type. We demonstrate that burst synchronization can be accompanied by complex, often chaotic, interactions of fast spikes within synchronized bursts.

  12. Dynamical constraints on the neutron star mass in EXO 0748-676

    OpenAIRE

    T. Muñoz-Darias(Oxford University, United Kingdom); Casares, J.; O'Brien, K.; Steeghs, D; Martinez-Pais, I. G.; Cornelisse, R.; Charles, P. A.

    2009-01-01

    We present VLT intermediate resolution spectroscopy of UY Vol, the optical counterpart of the LMXB X-ray burster EXO 0748-676. By using Doppler tomography we detect narrow components within the broad He II 4542 A, 4686 A and 5412 A emission lines. The phase, velocity and narrowness of these lines are consistent with their arising from the irradiated hemisphere of the donor star, as has been observed in a number of LMXBs. Under this assumption we provide the first dynamical constraints on the ...

  13. INTEGRAL reports renewed activity from KS 1741-293

    DEFF Research Database (Denmark)

    Chenevez, Jérôme; Kuulkers, E.; Alfonso-Garzón, J.; Beckmann, V.; Brandt, Søren Kristian; Bird, T.; Courvoisier, T.; Domingo, A.; Ebisawa, K.; Jonker, P.; Kretschmar, P.; Oosterbroek, T.; Paizis, A.; Sanchez-Fernandez, C.; Wijnands, R.

    2010-01-01

    The low-mass X-ray binary and burster source KS 1741-293 has been detected during recent INTEGRAL Galactic bulge (see ATel #438) observations by the JEM-X instrument. On February 25, 2010, between UTC 13:04 and 14:08, KS 1741-293 was detected at a 3-10 keV flux of 9 +/- 4 mCrab, and an upper limit...... the same energy bands. The region will be observed again by INTEGRAL on March 10, and the regular INTEGRAL monitoring of the Galactic bulge will continue until April 17, 2010. We encourage follow-up observations of this source at all wavelengths....

  14. POSSIBLE ADVERSE EFFECTS OF ONCE-DAILY ORAL THERAPEUTIC DOSE OF EITHER GLUCOSAMINE SULFATE OR GLUCOSAMINE/CHONDROITIN SULFATE ON BLOOD CELLS COUNT IN RATS

    Directory of Open Access Journals (Sweden)

    Noushi Abeer Amer

    2013-10-01

    Full Text Available This study was designed to investigate the possible adverse effects that may be induced by once-daily therapeutic doses of either glucosamine sulfate or glucosamine/chondroitin sulfate administered orally to rats for 30 days on blood cells (RBCs, WBCs and platelets counts. Forty three white healthy adult Albino rats of both sexes were selected randomly for this study. They were divided into three groups (І, ІІ, ІІІ. Group І received 0.05 ml distilled water, group ІІ received once daily therapeutic dose of glucosamine sulphate and group ІІІ received once daily therapeutic dose of glucosamine sulphate/chondroitin sulphate orally. The treatment period was for 30 days. At day 31, the animals were subjected to light ether anaesthesia and blood was withdrawn from the eye by retro-orbital puncture for the estimation of blood cells (RBCs, WBCs and platelets count. Treatment with single daily therapeutic dose of either GS alone or GS/CS for 30 days on blood cells count in rats produced a non significant change in RBCs counts compared to control and to each other. There were no statistically significant differences in total WBCs count at day 31 in animals administered once daily therapeutic dose of either GS or GS/CS orally compared to control group. In contrast, there was a statistically significant elevation in total WBCs count in GS/CS- treated rats compared to that in the GS-treated rats. The results of this study also showed that there was statistically significant decrease in neutrophils percentage in both drug treatment groups compared to control group. A statistically significant reduction in the percentage of monocytes was observed in GS/CS group compared to the corresponding percentage in animals of control group; while, there were non-significant differences in the percentage of monocytes in GS treated rats compared to that in the control group. There were no significant differences in the percentage of monocytes at day 31 of GS

  15. Oral and maxillofacial considerations in Gardner’s syndrome: a report of two cases

    Science.gov (United States)

    Pereira, Debora Lima; Carvalho, Paulo Andre; Achatz, Maria Isabel Waddington; Rocha, AndreCaroli; TardinTorrezan, Giovana; Alves, Fabio Abreu

    2016-01-01

    Gardner’s syndrome (GS) is a genetic disorder characterised by intestinal polyps, multiple osteomas, and soft-tissue tumours. Dentists play an important role in the syndrome diagnosis considering that craniomaxillofacial osteomas are a major criteria for Gardner’s syndrome diagnosis. This study aimed to describe the main stomatological manifestation of GS and the importance of dentists in its diagnosis. Two patients presenting GS were evaluated. The first one had two osteomas in the mandible and GS was suspected. The colonoscopy confirmed the presence of polyposis and a prophylactic proctocolectomy was performed. The other patient had a late-stage diagnosis of GS and developed a rectum adenocarcinoma. The presence of craniomaxillofacial osteomas are a hallmark of the disease. Early-stage GS diagnosis may enable early diagnosis and preventive strategies in carriers. Other dental abnormalities, such as supernumerary teeth, hypercementosis and odontomas, can also be observed. PMID:26981152

  16. Surface modification of poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) elastomer via covalent immobilization of nonionic sugar-based Gemini surfactants.

    Science.gov (United States)

    Xin, Zhirong; Du, Binbin; Yan, Shunjie; Du, Shanshan; Ding, Jiaotong; Yang, Zongfeng; Ren, Wanzhong

    2014-07-01

    Gemini surfactants (GS) with sugar-containing head-groups and different alkyl chains were successfully prepared. Poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) elastomer was grafted with glycidyl methacrylate (GMA) by means of UV-induced graft polymerization, and then the pGMA-grafted film was chemically immobilized with the GS. The surface graft polymerization was confirmed by ATR-FTIR and XPS. The wettability and hemocompatibility of the modified surface were characterized by means of water contact angle, protein adsorption, and platelet adhesion assays. The results showed that amphiphilic surfactant-containing polymer surfaces presented protein-resistant behavior and anti-platelet adhesion after functionalization with GS, GS1 and GS2. Besides, the hemocompatibility of the modified surface deteriorated as the length of hydrophobic chain of GS increased. PMID:24854325

  17. GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

    Institute of Scientific and Technical Information of China (English)

    徐迎利; 苗宏志; 刘振辉; 邓勇; 兰山; 王尧; 张培军; 徐斌; 麦康森

    2001-01-01

    Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth.

  18. GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer matrix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.

  19. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

    Directory of Open Access Journals (Sweden)

    Takuya Sato

    2015-07-01

    Full Text Available Mouse spermatogonial stem cells (SSCs can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS cells, are targets of genome modification, this process remains technically difficult. In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure.

  20. One-step synthesis of graphene/SnO{sub 2} nanocomposites and its application in electrochemical supercapacitors

    Energy Technology Data Exchange (ETDEWEB)

    Li Fenghua; Song Jiangfeng; Yang Huafeng; Gan Shiyu; Zhang Qixian; Han Dongxue; Niu Li [State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China); Ivaska, Ari, E-mail: lniu@ciac.jl.c [Laboratory of Analytical Chemistry, Process Chemistry Centre, Abo Akademi University, FI-20500 Abo-Turku (Finland)

    2009-11-11

    A one-step method was developed to fabricate conductive graphene/SnO{sub 2} (GS) nanocomposites in acidic solution. Graphite oxides were reduced by SnCl{sub 2} to graphene sheets in the presence of HCl and urea. The reducing process was accompanied by generation of SnO{sub 2} nanoparticles. The structure and composition of GS nanocomposites were confirmed by means of transmission electron microscopy, x-ray photoelectron and Raman spectroscopy. Moreover, the ultracapacitor characteristics of GS nanocomposites were studied by cyclic voltammograms (CVs) and electrical impedance spectroscopy (EIS). The CVs of GS nanocomposites are nearly rectangular in shape and the specific capacitance degrades slightly as the voltage scan rate is increased. The EIS of GS nanocomposites presents a phase angle close to {pi}/2 at low frequency, indicating a good capacitive behavior. In addition, the GS nanocomposites could be promisingly applied in many fields such as nanoelectronics, ultracapacitors, sensors, nanocomposites, batteries and gas storage.