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Sample records for bunyaviridae phlebovirus transmitidos

  1. Characterization of the Bhanja Serogroup Viruses (Bunyaviridae): a Novel Species of the Genus Phlebovirus and Its Relationship with Other Emerging Tick-Borne Phleboviruses

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    Matsuno, Keita; Weisend, Carla; Travassos da Rosa, Amelia P. A.; Anzick, Sarah L.; Dahlstrom, Eric; Porcella, Stephen F.; Dorward, David W.; Yu, Xue-Jie; Tesh, Robert B.

    2013-01-01

    Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans. PMID:23325688

  2. Aguacate virus, a new antigenic complex of the genus Phlebovirus (family Bunyaviridae).

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    Palacios, Gustavo; da Rosa, Amelia Travassos; Savji, Nazir; Sze, Wilson; Wick, Ivan; Guzman, Hilda; Hutchison, Stephen; Tesh, Robert; Lipkin, W Ian

    2011-06-01

    Genomic and antigenic characterization of Aguacate virus, a tentative species of the genus Phlebovirus, and three other unclassified viruses, Armero virus, Durania virus and Ixcanal virus, demonstrate a close relationship to one another. They are distinct from the other nine recognized species within the genus Phlebovirus. We propose to designate them as a new (tenth) serogroup or species (Aguacate virus) within the genus. The four viruses were all isolated from phlebotomine sandflies (Lutzomyia sp.) collected in Central and South America. Aguacate virus appears to be a natural reassortant and serves as one more example of the high frequency of reassortment in this genus. PMID:21325481

  3. [Genetic characterization of the Sakhalin virus (SAKV), Paramushir virus (PMRV) (Sakhalin group, Nairovirus, Bunyaviridae), and Rukutama virus (RUKV) (Uukuniemi group, Phlebovirus, Bunyaviridae) isolated from the obligate parasites of the colonial sea-birds ticks Ixodes (Ceratixodes) uriae, White 1852 and I. signatus Birulya, 1895 in the water area of sea of the Okhotsk and Bering sea].

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    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Aristova, V A; Gitel'man, A K; Samokhvalov, E I; Botikov, A G

    2014-01-01

    Full-length genomes of the Sakhalin virus (SAKH) and Paramushir virus (PRMV) (Sakhalin group, Nairovirus, Bunyaviridae) isolated from the ticks Ixodes uriae White 1852 were sequenced using the next-generation sequencing (Genbank ID: KF801659, KF801656). SAKV and PRMV have 81% identity for the part of RNA-dependent RNA-polymerase (RdRp) on the nucleotide level and 98.5% on the amino acid level. Full-length genome comparison shows that SAKV have, in average, from 25% (N-protein, S-segment) to 50% (RdRp, L-segment) similarity with the nairoviruses. The maximum value of the amino acid similarity (50.3% for RdRp) SAKV have with the Crimean-Congo hemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV), which are also associated with the Ixodidae ticks. Another virus studied is Rukutama virus (RUKV) (isolated from ticks I. signatus Birulya, 1895) that recently was classified (based on morphology and antigenic reaction) to the Nairovirus genus, presumably to the Sakhalin group. In this work the genome of the RUKV was sequenced (KF892052-KF892054) and RUKV was classified as a member of the Uukuniemi group (Phlebovirus, Bunyaviridae). RUKV is closely related (93.0-95.5% similarity) with our previously described Komandory virus (KOMV). RUKV and KOMV form separate phylogenetic line neighbor of Manawa virus (MWAV) isolated from the ticks Argas abdussalami Hoogstraal et McCarthy, 1965 in Pakistan. The value of the similarity between RUCV and MWAV is 65-74% on the amino acid level. PMID:25335413

  4. RNA Encapsidation and Packaging in the Phleboviruses.

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    Hornak, Katherine E; Lanchy, Jean-Marc; Lodmell, J Stephen

    2016-01-01

    The Bunyaviridae represents the largest family of segmented RNA viruses, which infect a staggering diversity of plants, animals, and insects. Within the family Bunyaviridae, the Phlebovirus genus includes several important human and animal pathogens, including Rift Valley fever virus (RVFV), severe fever with thrombocytopenia syndrome virus (SFTSV), Uukuniemi virus (UUKV), and the sandfly fever viruses. The phleboviruses have small tripartite RNA genomes that encode a repertoire of 5-7 proteins. These few proteins accomplish the daunting task of recognizing and specifically packaging a tri-segment complement of viral genomic RNA in the midst of an abundance of host components. The critical nucleation events that eventually lead to virion production begin early on in the host cytoplasm as the first strands of nascent viral RNA (vRNA) are synthesized. The interaction between the vRNA and the viral nucleocapsid (N) protein effectively protects and masks the RNA from the host, and also forms the ribonucleoprotein (RNP) architecture that mediates downstream interactions and drives virion formation. Although the mechanism by which all three genomic counterparts are selectively co-packaged is not completely understood, we are beginning to understand the hierarchy of interactions that begins with N-RNA packaging and culminates in RNP packaging into new virus particles. In this review we focus on recent progress that highlights the molecular basis of RNA genome packaging in the phleboviruses. PMID:27428993

  5. The Role of Phlebovirus Glycoproteins in Viral Entry, Assembly and Release.

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    Spiegel, Martin; Plegge, Teresa; Pöhlmann, Stefan

    2016-01-01

    Bunyaviruses are enveloped viruses with a tripartite RNA genome that can pose a serious threat to animal and human health. Members of the Phlebovirus genus of the family Bunyaviridae are transmitted by mosquitos and ticks to humans and include highly pathogenic agents like Rift Valley fever virus (RVFV) and severe fever with thrombocytopenia syndrome virus (SFTSV) as well as viruses that do not cause disease in humans, like Uukuniemi virus (UUKV). Phleboviruses and other bunyaviruses use their envelope proteins, Gn and Gc, for entry into target cells and for assembly of progeny particles in infected cells. Thus, binding of Gn and Gc to cell surface factors promotes viral attachment and uptake into cells and exposure to endosomal low pH induces Gc-driven fusion of the viral and the vesicle membranes. Moreover, Gn and Gc facilitate virion incorporation of the viral genome via their intracellular domains and Gn and Gc interactions allow the formation of a highly ordered glycoprotein lattice on the virion surface. Studies conducted in the last decade provided important insights into the configuration of phlebovirus Gn and Gc proteins in the viral membrane, the cellular factors used by phleboviruses for entry and the mechanisms employed by phlebovirus Gc proteins for membrane fusion. Here, we will review our knowledge on the glycoprotein biogenesis and the role of Gn and Gc proteins in the phlebovirus replication cycle. PMID:27455305

  6. The Role of Phlebovirus Glycoproteins in Viral Entry, Assembly and Release

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    Martin Spiegel

    2016-07-01

    Full Text Available Bunyaviruses are enveloped viruses with a tripartite RNA genome that can pose a serious threat to animal and human health. Members of the Phlebovirus genus of the family Bunyaviridae are transmitted by mosquitos and ticks to humans and include highly pathogenic agents like Rift Valley fever virus (RVFV and severe fever with thrombocytopenia syndrome virus (SFTSV as well as viruses that do not cause disease in humans, like Uukuniemi virus (UUKV. Phleboviruses and other bunyaviruses use their envelope proteins, Gn and Gc, for entry into target cells and for assembly of progeny particles in infected cells. Thus, binding of Gn and Gc to cell surface factors promotes viral attachment and uptake into cells and exposure to endosomal low pH induces Gc-driven fusion of the viral and the vesicle membranes. Moreover, Gn and Gc facilitate virion incorporation of the viral genome via their intracellular domains and Gn and Gc interactions allow the formation of a highly ordered glycoprotein lattice on the virion surface. Studies conducted in the last decade provided important insights into the configuration of phlebovirus Gn and Gc proteins in the viral membrane, the cellular factors used by phleboviruses for entry and the mechanisms employed by phlebovirus Gc proteins for membrane fusion. Here, we will review our knowledge on the glycoprotein biogenesis and the role of Gn and Gc proteins in the phlebovirus replication cycle.

  7. Differential Use of the C-Type Lectins L-SIGN and DC-SIGN for Phlebovirus Endocytosis.

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    Léger, Psylvia; Tetard, Marilou; Youness, Berthe; Cordes, Nicole; Rouxel, Ronan N; Flamand, Marie; Lozach, Pierre-Yves

    2016-06-01

    Bunyaviruses represent a growing threat to humans and livestock globally. The receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely unidentified and poorly characterized. DC-SIGN is a C-type lectin highly expressed on dermal dendritic cells that has been found to act as an authentic entry receptor for many phleboviruses (Bunyaviridae), including Rift Valley fever virus (RVFV), Toscana virus (TOSV) and Uukuniemi virus (UUKV). We found that these phleboviruses can exploit another C-type lectin, L-SIGN, for infection. L-SIGN shares 77% sequence homology with DC-SIGN and is expressed on liver sinusoidal endothelial cells. L-SIGN is required for UUKV binding but not for virus internalization. An endocytosis-defective mutant of L-SIGN was still able to mediate virus uptake and infection, indicating that L-SIGN acts as an attachment receptor for phleboviruses rather than an endocytic receptor. Our results point out a fundamental difference in the use of the C-type lectins L-SIGN and DC-SIGN by UUKV to enter cells, although both proteins are closely related in terms of molecular structure and biological function. This study sheds new light on the molecular mechanisms by which phleboviruses target the liver and also highlights the added complexity in virus-receptor interactions beyond attachment. PMID:26990254

  8. Inhibition of sandfly fever Sicilian virus (Phlebovirus) replication in vitro by antiviral compounds.

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    Crance, J M; Gratier, D; Guimet, J; Jouan, A

    1997-01-01

    Sandfly fever Sicilian virus (SFSV) was used in our laboratory to screen antiviral substances active toward viruses of the Bunyaviridae family. Antiviral activity was estimated by the reduction of the cytopathic effect of SFSV on infected Vero cells. Cytotoxicity was evaluated by determining the inhibition of Trypan blue exclusion. The specificity of action of each tested compound was estimated by the selectivity index (CD50/ED50). Selectivity indices of human recombinant interferon-alpha (IFN alpha) (Roferon and Introna), iota-, kappa- and lambda- carrageenans, fucoidan and 6-azauridine were much higher than that of ribavirin, the only antiviral substance which has been previously investigated for its inhibitory effects on Phlebovirus infections. Other compounds showed significant antiviral activity: glycyrrhizin, suramin sodium, dextran sulphate and pentosan polysulphate. All these compounds caused a concentration-dependent reduction in the virus yield. Ribavirin, 6-azauridine and IFN alpha have been shown to inhibit a late step of the virus replicative cycle, whereas glycyrrhizin and suramin sodium were active at an early step and the sulphated polysaccharides inhibited adsorption of SFSV on the cells. The antiviral compounds selected in this study as specific inhibitors of in vitro replication of SFSV are promising candidates for the chemotherapy of haemorrhagic fevers caused by viruses of the Bunyaviridae family. The combination of IFN alpha and ribavirin, which showed a synergistic antiviral effect, should be evaluated for the treatment of these infections. PMID:9403935

  9. Development of pan-phlebovirus RT-PCR assay.

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    Klimentov, Alexander S; Butenko, Alexander M; Khutoretskaya, Natalia V; Shustova, Elena Yu; Larichev, Victor F; Isaeva, Olga V; Karganova, Galina G; Lukashev, Alexander N; Gmyl, Anatoly P

    2016-06-01

    This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples. PMID:26947398

  10. Complete Genome Characterization of the Arumowot Virus (Unclassified Phlebovirus) Isolated from Turdus libonyanus Birds in the Central African Republic.

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    Berthet, Nicolas; Nakouné, Emmanuel; Gessain, Antoine; Manuguerra, Jean-Claude; Kazanji, Mirdad

    2016-02-01

    The Bunyaviridae family is currently composed of five genera, including Phlebovirus, in which several phleboviruses are associated with human diseases. Using high-throughput sequencing, we obtained and characterized one complete genome of the Arumowot virus (AMTV) isolated in 1978 from Turdus libonyanus, the Kurrichane Thrush, in the Central African Republic (CAR). The genomic segment of the new strain of AMTV isolated in the CAR had 75.4-83.5% sequence similarity and 82-98.4% amino acid similarity to the prototype sequence of AMTV. The different conserved proteins of the small (S) and large (L) segments (Nc, NSP, and RNA polymerase) showed close similarity at the amino acid level, whereas the polyprotein of the medium (M) segment was highly divergent, with 18% and 37.7%, respectively, for the prototype sequence of AMTV and the Odrenisrou virus (ODRV) isolated from Culex (Cx.) albiventris mosquitoes in the Tai forest, Ivory Coast. Phylogenetic analysis confirmed the sequence homology analysis and indicated that AMTV-CAR clustered into the Salehabad virus antigenic complex. The two closest viruses were the prototype sequences of AMTV originally isolated from Cx. antennatus mosquitoes and ODRV. These molecular data suggest the need for a deep genetic characterization of the diversity of this viral species to enhance its detection in the Central African region and to understand better its behavior and life cycle so that its potential spread to the human population can be prevented. PMID:26807610

  11. PREVALENCE OF ARBOVIRUS ANTIBODIES AGAINST THE FAMILY Bunyaviridae IN WATER BUFFALOES

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    Alexandre Rosário Casseb

    2015-07-01

    Full Text Available The State of Pará comprises 26% of Brazilian Amazon region where a large diversity of arboviruses has been described. This study sought to assess the prevalence and distribution of haemagglutination-inhibition antibodies against antigens of nine different types of arbovirus of the Bunyaviridae family, where eight were Orthobunyavirus: Guaroa virus, Maguari virus, Tacaiuma virus, Utinga virus, Belem virus, Caraparu virus, Oropouche virus and Catu virus, and one Phlebovirus: Icoaraci virus in sera samples of water buffaloes in Pará State, Brazil. For all Arboviruses investigated there were antibodies, with the exception of Belem virus. Antibodies to Maguari virus were more prevalent (7.33%. The water buffaloes of the present study showed variable levels of antibodies in monotypic and heterotypic reactions that may indicate there are movements from most bunyavirus studied in domestic buffaloes in the state of Pará, and the Maguari virus presents the largest circulation. Therefore, further studies are needed to investigate the role of water buffalo in the maintenance and dispersal of arboviruses, as well as whether these viruses can cause disease in that species, especially in cases of birth defects and abortions.

  12. Anticuerpos frente a virus West nile y otros virus transmitidos por artropodos en la poblacion del Delta del Ebro

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    Lozano Alvaro

    1998-01-01

    Full Text Available FUNDAMENTOS: El virus West Nile (VWN es un Flavivirus que se transmite al hombre a través de distintas especies de mosquitos y produce brotes y casos esporádicos de enfermedad en distintas regiones del Viejo Mundo, incluída la Cuenca Mediterránea. Las zonas húmedas europeas que acogen aves migratorias procedentes de África constituyen áreas de alto riesgo para esta infección, así como para otras infecciones víricas transmitidas por artrópodos. MÉTODOS: Con objeto de investigar la prevalencia de la infección por el VWN y otros virus de transmisión similar en la población humana del Delta del Ebro, se estudiaron 1037 muestras de suero, obtenidas en 10 localidades de la zona, para presencia de anticuerpos frente a VWN y otros 12 virus transmitidos por artrópodos (3 Alfavirus, 8 Flaviviridae y 1 Bunyaviridae mediante titulación por inhibición de la hemaglutinación (IHA. En algunos casos se estudió la presencia de IgM específica por IHA tras fraccionar el suero por centrifugación en gradientes de sacarosa. RESULTADOS: En total, se encontró reactividad significativa frente a alguno de los virus probados en 130 casos (12.5%; 4.1% frente a Alfavirus, 8.0% frente a Flaviviridae y 0.4% frente a Bunyaviridae. El análisis de los títulos de anticuerpos reveló porcentajes significativos de muestras con títulos elevados frente a antígenos de VWN y otros. La distribución de la seroprevalencia fue muy desigual, concentrándose fundamentalmente en 3 localidades del interior del Delta (Ampolla, San Jaime y Montells, donde la prevalencia de anticuerpos frente a Flaviviridae llegó a alcanzar el 30% y se observaron niveles residuales de IgM frente a VWN en algunos sueros. CONCLUSIONES: Estos resultados y los obtenidos previamente en otras regiones de la Península Ibérica sugieren que el VWN circula en la población humana de las zonas de riesgo y produce brotes epidémicos periódicos. Habida cuenta del alto porcentaje de

  13. [Molecular epidemiology of phlebovirus in four provinces in Morocco].

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    Es-Sette, N; Ajaoud, M; Charrel, R N; Lemrani, M

    2016-08-01

    Sandflies are vectors of protozoa, viruses, and bacteria. To investigate the transmission of phleboviruses, a total of 8753 sandflies were collected in four foci of leishmania. A total of 16 distinct species were morphologically identified. Nested-PCR and cell culture screening for phleboviruses, using an assay targeting the polymerase gene, showed positive results for 19 pools of sandflies. Sequencing of the corresponding products confirmed the results and allowed identification of Toscana virus exclusively. Corresponding sandfly species originated from four different foci, and were different from those commonly reported in the literature. Sequence analysis shows that the Moroccan Toscana viruses belong to genotype B and appear close to the Toscana viruses isolated in France and Spain. This study reported the existence of the virus in the north, center and south of the country. The abundance and diversity of sandflies in Morocco, Mediterranean climate, would support the continuous circulation of Toscana virus in our country, posing a potential risk of emergence of these arboviruses. PMID:27193286

  14. Phleboviruses associated with sand flies in arid bio-geographical areas of Central Tunisia.

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    Dachraoui, K; Fares, W; Bichaud, L; Barhoumi, W; Beier, J C; Derbali, M; Cherni, S; Lamballerie, X de; Chelbi, I; Charrel, R N; Zhioua, E

    2016-06-01

    An entomological investigation was carried out in 2014 at two sites located in Central Tunisia, one irrigated and another non-irrigated situated in arid bio-geographical areas. Sand flies of the subgenus Larroussius namely Phlebotomus perfiliewi, Phlebotomus perniciosus, and Phlebotomus longicuspis are the most abundant sand fly species in the irrigated site. However, in the non-irrigated site, Phlebotomus papatasi of the Phlebotomus genus is the most abundant species. A total of 3191 sand flies were collected and pooled with up to 30 specimens per pool based on sex, trapping location and collection date, were tested for the presence of phleboviruses by nested reverse transcriptase polymerase chain reaction in the polymerase gene and sequenced. Of a total of 117 pools, 4 were positive, yielding a minimum infection rate of sand flies with phleboviruses of 0.12%. Phylogenetic analysis performed using partial nucleotide and amino acid sequence in the polymerase gene showed that these phleboviruses belonged to four different clusters corresponding to Toscana virus (TOSV), Saddaguia virus (SADV), Sandfly Fever Sicilian Virus (SFSV) and Utique virus (UTIV). This study provides more evidence that the abundance of P. perfiliewi is associated with the development of irrigation in arid bio-geographical areas of Central Tunisia which may have led to the emergence of phleboviruses. We report the first detection of TOSV from sand flies collected from Central Tunisia. PMID:26875761

  15. 布尼亚病毒及其引发的疾病%Bunyaviridae and Its Diseases

    Institute of Scientific and Technical Information of China (English)

    邵惠训

    2011-01-01

    The bunyaviridae family comprises more than 300 viruses. Membership is usually based on antigenic interrelatedness or morphological similarity. Disease characterized by fever, headache, weakness, myalgia, pulmonary edema. The family is divided into 5 genera; 1. Orthobunyavirus: Bunyamwera, La Cross, Tahyna virus, transmitted mainly by mosquitoes. 2. Hantavirus; Hantaan virus, transmission does not require insects. 3. Nairovirus; Dugbe virus infection of cattle in West Africa, transmitted by ticks. 4. Phlebovirus;Sandfly fever, Rift valley fever, transmitted by sandflies. 5. Tospovirus; Tomato spotted wilt virus,only infect plant and non-vertebrate. Man is not known to be a natural or reservoir for any of these viruses. Virions are 80 ~ 120 nm in diameter, 5 ~ 10 nm projections visible on the surface. Genome consists of 3 pieces of negative stranded RNA. Virion has 2 surface glycoproteins Cl and C2, with HA and virus neutralization epitopes. Bunyaviridae is a family of negative stranded RNA viruses. Though generally found in arthropode or rodents, certain viruses in this family occasionally infect humans. Bunyaviridae are vector-borne viruses. With the exception of Hantaviruses transmission occurs via an arthropod vector. Hantaviruses are transmitted through contact with mice feces. Crimean-Congo hemorrhagic fever virus is associated with high levels of morbidity and mortality, consequently handling of these viruses most occurs with a biosafe level 4 laboratory. Hantavirus or Hantavirus hemorrhagic fever, common in China, Korea, Scandinavia, Russia, and the American southwest, is associated with high fever, lung edema and pulmonary failture. Mortality is around 55% of laboratory diagnosis of bunya virus infections. Virus isolation-intra-cranialinoculation of suckling mice is thought to be the most sensitive system available for virus isolation. However sensitive cell culture systems are available such as Vero, Vero E6, A549 and mosquito cells. Once isolated the

  16. Diagnóstico virológico y molecular de virus transmitidos por roedores. Hantavirus y arenavirus

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    Silvana Levis

    2010-01-01

    Los hantavirus (familia Bunyaviridae) y arenavirus (familia Arenaviridae) son virus de roedores; cada uno de ellos parece estar estrictamente asociado con una especie de roedor en la que causa una infección persistente y asintomática. En las Américas tienen como reservorios primarios a roedores de la sub-familia Sigmodontinae, y son causantes de síndrome pulmonar por Hantavirus (SPH) y fiebres hemorrágicas, respectivamente (1,2). El número de estos virus identificados en los últimos años ha a...

  17. Diagnóstico virológico y molecular de virus transmitidos por roedores. Hantavirus y arenavirus

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    Silvana Levis

    2010-04-01

    Full Text Available Los hantavirus (familia Bunyaviridae y arenavirus (familia Arenaviridae son virus de roedores; cada uno de ellos parece estar estrictamente asociado con una especie de roedor en la que causa una infección persistente y asintomática. En las Américas tienen como reservorios primarios a roedores de la sub-familia Sigmodontinae, y son causantes de síndrome pulmonar por Hantavirus (SPH y fiebres hemorrágicas, respectivamente (1,2. El número de estos virus identificados en los últimos años ha aumentado significativamente; actualmente, el género Hantavirus está compuesto por más de 28 tipos diferentes, mientras que al menos 23 arenavirus conforman el género Arenavirus. Entre los hantavirus asociados con SPH se destacan el virus Sin Nombre en Norteamérica, y los virus Andes, Laguna Negra, Caño Delgadito, Araraquara y Juquitiba, en el cono sur de América, entre otros (2. Los arenavirus asociados a fiebres hemorrágicas reconocidos en Sud América al presente son: Junín (Argentina, Guanarito (Venezuela, Sabiá (Brasil, y Machupo y Chapare (Bolivia (3.

  18. Isolation of Madre de Dios Virus (Orthobunyavirus; Bunyaviridae), an Oropouche Virus Species Reassortant, from a Monkey in Venezuela.

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    Navarro, Juan-Carlos; Giambalvo, Dileyvic; Hernandez, Rosa; Auguste, Albert J; Tesh, Robert B; Weaver, Scott C; Montañez, Humberto; Liria, Jonathan; Lima, Anderson; Travassos da Rosa, Jorge Fernando Soares; da Silva, Sandro P; Vasconcelos, Janaina M; Oliveira, Rodrigo; Vianez, João L S G; Nunes, Marcio R T

    2016-08-01

    Oropouche virus (OROV), genus Orthobunyavirus, family Bunyaviridae, is an important cause of human illness in tropical South America. Herein, we report the isolation, complete genome sequence, genetic characterization, and phylogenetic analysis of an OROV species reassortant, Madre de Dios virus (MDDV), obtained from a sick monkey (Cebus olivaceus Schomburgk) collected in a forest near Atapirire, a small rural village located in Anzoategui State, Venezuela. MDDV is one of a growing number of naturally occurring OROV species reassortants isolated in South America and was known previously only from southern Peru. PMID:27215299

  19. Development of FGI-106 as a broad-spectrum therapeutic with activity against members of the family Bunyaviridae

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    Darci R Smith

    2010-02-01

    Full Text Available Darci R Smith1, Monica Ogg1, Aura Garrison1, Abdul Yunus2, Anna Honko1, Josh Johnson1, Gene Olinger1, Lisa E Hensley1, Michael S Kinch1United States Army Medical Research Institute of Infectious Diseases (USAMRII D, Fort Detrick, MD, USA; 2Functional Genetics, Inc., Gaithersburg, MD, USAAbstract: The family Bunyaviridae is a diverse group of negative-strand RNA viruses that infect a wide range of arthropod vectors and animal hosts. Based on the continuing need for new therapeutics to treat bunyavirus infections, we evaluated the potential efficacy of FGI-106, a small-molecular compound that previously demonstrated activity against different RNA viruses. FGI-106 displayed substantial antiviral activity in cell-based assays of different bunyavirus family members, including Asian and South American hantaviruses (Hantaan virus and Andes virus, Crimean-Congo hemorrhagic fever virus, La Crosse virus, and Rift Valley fever virus. The pharmacokinetic profile of FGI-106 revealed sufficient exposure of the drug to critical target organs (lung, liver, kidney, and spleen, which are frequently the sites of bunyavirus replication. Consistent with these findings, FGI-106 treatment delivered via intraperitoneal injection prior to virus exposure was sufficient to delay the onset of Rift Valley fever virus infection in mouse-based models and to enhance survival in the face of an otherwise lethal infection. Altogether, these results suggest a potential opportunity for the use of FGI-106 to treat infections by members of the family Bunyaviridae.Keywords: Rift Valley fever virus, bunyavirus, hantavirus, antiviral, therapeutic

  20. Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae.

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    Ladner, Jason T; Savji, Nazir; Lofts, Loreen; Travassos da Rosa, Amelia; Wiley, Michael R; Gestole, Marie C; Rosen, Gail E; Guzman, Hilda; Vasconcelos, Pedro F C; Nunes, Marcio R T; J Kochel, Tadeusz; Lipkin, W Ian; Tesh, Robert B; Palacios, Gustavo

    2014-05-01

    A thorough characterization of the genetic diversity of viruses present in vector and vertebrate host populations is essential for the early detection of and response to emerging pathogenic viruses, yet genetic characterization of many important viral groups remains incomplete. The Simbu serogroup of the genus Orthobunyavirus, family Bunyaviridae, is an example. The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Here, we report complete genome sequences for 11 viruses within this group, with a focus on the large and poorly characterized Manzanilla and Oropouche species complexes. Phylogenetic and pairwise divergence analyses indicated the presence of high levels of genetic diversity within these two species complexes, on a par with that seen among the five other species complexes in the Simbu serogroup. Based on previously reported divergence thresholds between species, the data suggested that these two complexes should actually be divided into at least five species. Together these five species formed a distinct phylogenetic clade apart from the rest of the Simbu serogroup. Pairwise sequence divergences among viruses of this clade and viruses in other Simbu serogroup species complexes were similar to levels of divergence among the other orthobunyavirus serogroups. The genetic data also suggested relatively high levels of natural reassortment, with three potential reassortment events present, including two well-supported events involving viruses known to infect humans. PMID:24558222

  1. Eficacia del Albendazol en dosis única sobre las infecciones por helmintos transmitidos por el suelo en escolares de una comunidad de Iquitos, Perú

    Directory of Open Access Journals (Sweden)

    Theresa W Gyorkos

    2013-10-01

    Full Text Available Objetivos. Determinar la eficacia en dosis única del albendazol sobre las infecciones por helmintos transmitidos por el suelo (HTS en escolares de una comunidad de la ciudad de Iquitos en Perú. Materiales y métodos. Dentro del contexto de un ensayo controlado aleatorizado realizado en una comunidad periurbana de escasos recursos, situada en Iquitos, en la Amazonía de Perú, se obtuvieron muestras de heces de escolares del quinto grado de primaria en 18 escuelas y se analizó la prevalencia y la intensidad de HTS. Un total de 1193 escolares fueron desparasitados con albendazol en dosis única (400 mg. De los 909 escolares que fueron encontrados positivos con al menos una infección por HTS, una muestra aleatoria de 385 fue seguida dos semanas más tarde, cuando se recolectó y analizó una segunda muestra de heces. Resultados. La eficacia del albendazol fue satisfactoria para las infecciones por Ascaris lumbricoides con una tasa de reducción de huevos (TRH de 99,8%; IC 95: 99,3-100 y por anquilostomideos con una TRH de 93,6%, IC 95%: 88,2-96,6 y por Trichuris trichiura con una TRH de 72,7%, IC 95: 58,5-79,1. Conclusiones. Estos resultados son indicativos de niveles satisfactorios de eficacia y son congruentes con datos publicados sobre la eficacia del albendazol y directivas de la Organización Mundial de la Salud. Futuras investigaciones deben centrarse en mejorar la eficacia de las estrategias de tratamiento para la infección por Trichuris trichiura.

  2. Caracterização e relacionamento antigênico de três novos Bunyavirus no grupo Anopheles A (Bunyaviridae dos arbovirus Characterization and antigenic relationship of three new Bunyavirus in the Anopheles A serogroup (Bunyaviridae of arboviruses

    Directory of Open Access Journals (Sweden)

    Jorge Fernando Soares Travassos da Rosa

    1992-06-01

    Full Text Available São descritos o isolamento e a caracterização de três novos arbovirus isolados na região da Usina Hidro-Elétrica de Tucuruí (UHE-TUC. Os três novos arbovirus pertencem ao grupo Anopheles A(ANA, gênero Bunyavirus (família Bunyaviridae. Os vírus Tucuruí (TUC, Caraipé (CPE e Arumateua (ART são relacionados entre si e com o vírus Trombetas (TBT, formando dentro do grupo ANA um complexo chamado Trombetas. Os arbovirus TUC, CPE e ART foram obtidos a partir de lotes de mosquitos Anopheles (Nyssorhynchus sp capturados em Tucuruí, nas proximidades da usina hidrelétrica de Tucuruí, Estado do Pará, nos meses de fevereiro, agosto e outubro de 1984, respectivamente. Até o final de 1990 os vírus TUC, CPE e ART foram isolados 12, 32 e 28 vezes respectivamente, sempre na região da UHE-TUC, exceção feita ao vírus TUC, do qual se obteve uma amostra procedente de Balbina, onde também foi construída uma hidroelétrica. Até o presente, esses vírus só foram isolados a partir de mosquitos do grupo An. (Nys. principalmente, a partir das espécies An. (Nys. nuneztovari e An. (Nys. triannulatus também consideradas vetores secundários da malária na Amazônia Brasileira. Testes sorológicos executados com soros humanos e de diversas espécies de animais silvestres foram negativos, com exceção de um soro de um carnívoro de espécie Nasua nasua que neutralizou a amostra TUC em títulos de 2.6 índice logaritmico de neutralização (ILN.The isolation and characterization of three new viruses obtained from the Tucuruí hydroelectric dam region is repeated. These three agents belong to the Anopheles A serogroup, genus Bunyavirus, Bunyaviridae. The Tucuruí (TUC, Caraipe (CPE and Arumateua (ART viruses have close relationships with each other and with Trombetas (TBT virus, an Anopheles A virus previously isolated in the Amazon Region of Brazil. These viruses form the "Trombetas complex". TUC, CPE and ART viruses were obtained from pools of

  3. Identificación y caracterización molecular de virus transmitidos por mosca blanca bemisia tabaci que infectan tomate en la región andina de Colombia

    OpenAIRE

    Betancur Pérez, Jhon Fredy

    2012-01-01

    Los Begomovirus son virus que pertenecen a la familia Geminiviridae, se caracterizan por infectar plantas dicotiledóneas y ser transmitidos por mosca blanca (Bemisia tabaci, biotipo B). El genoma está constituido por una o dos moléculas circulares de ADN de cadena sencilla, cuyos genes están asociados con procesos de replicación, transcripción, encapsidación y movimiento. Los Begomovirus son considerados virus emergentes debido al aumento en la incidencia y severidad en las enfermedades causa...

  4. Resistencia genética al Virus del Arrugamiento Foliar del Fríjol transmitido por Bemisia tabaci (Hemiptera: Aleyrodidae) Genetic resistance to Bean Leaf Crumple Virus transmitted by Bemisia tabaci (Hemiptera: Aleyrodidae)

    OpenAIRE

    MARÍA ELENA CUÉLLAR J.; Morales, Francisco J.; JAMES MONTOYA LERMA

    2011-01-01

    Desde 2002, los cultivos de fríjol habichuela (Phaseolus vulgaris) de la parte plana del Valle del Cauca han sido afectados por el Virus del Arrugamiento Foliar del Fríjol (Begomovirus: Geminiviridae) agente causal de una nueva y severa enfermedad, transmitido por el biotipo B de la mosca blanca, Bemisia tabaci. Se investigó la relación virus-vector utilizando genotipos de fríjol común seleccionados como posibles fuentes de resistencia. Se determinó la eficiencia de transmisión del virus usan...

  5. Los Annales martyrum transmitidos por Madrid, BN, 10029 y Madrid, BRAH, 78: edición, estudio y panorámica de su infl uencia en la literatura analística latina de la Hispania medieval

    Directory of Open Access Journals (Sweden)

    Martín, José Carlos

    2011-06-01

    Full Text Available The scholar analyzes and publishes the Latin annals of martyrs transmitted in Madrid, BN, 10029 and, in an unpublished second version, Madrid, BRAH, 78. He also examines the possible date and origin, sources and influence in the Latin annals of Iberian Peninsule. Finally, this paper offers new editions of the so-called Chronicon Rotense alterum and Chronicon paruum Ambrosianum.Se estudian y editan en este artículo unos anales de mártires transmitidos por Madrid, BN, 10029 y, en una segunda redacción inédita, por Madrid, BRAH, 78. Además se examina su posible datación y origen, sus fuentes y su influencia en la literatura analística latina de la Península Ibérica. Se ofrecen asimismo nuevas ediciones de los denominados Chronicon Rotense alterum y Chronicon paruum Ambrosianum.

  6. Caracterização e relacionamento antigênico de três novos Bunyavirus no grupo Anopheles A (Bunyaviridae) dos arbovirus

    OpenAIRE

    Rosa Jorge Fernando Soares Travassos da; Rosa Amélia Paes de Andrade Travassos da; Dégallier Nicolas; Vasconcelos Pedro Fernando da Costa

    1992-01-01

    São descritos o isolamento e a caracterização de três novos arbovirus isolados na região da Usina Hidro-Elétrica de Tucuruí (UHE-TUC). Os três novos arbovirus pertencem ao grupo Anopheles A(ANA), gênero Bunyavirus (família Bunyaviridae). Os vírus Tucuruí (TUC), Caraipé (CPE) e Arumateua (ART) são relacionados entre si e com o vírus Trombetas (TBT), formando dentro do grupo ANA um complexo chamado Trombetas. Os arbovirus TUC, CPE e ART foram obtidos a partir de lotes de mosquitos Anopheles (Ny...

  7. [Taxonomic status of the Burana virus (BURV) (Bunyaviridae, Nairovirus, Tamdy group) isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 and Haem. concinna Koch, 1844 (Ixodidae, Haemaphysalinae) in Kyrgyzstan].

    Science.gov (United States)

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Gitel'man, A K; Aristova, V A; Botikov, A G

    2014-01-01

    Complete genome sequence of the Burana virus (BURV) was determined using the next-generation sequencing approach (ID GenBank KF801651). The prototype strain of BURV LEIV-Krg760 was originally isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 (Ixodidae, Haemaphysalinae), collected from cows in Tokmak wildlife sanctuary, eastern part of the Chu valley (43 degrees 10' N, 74 degrees 40' E) near Burana village, Kirgizia, in April 1971. Molecular genetics and phylogenetic analyses showed that the BURV belonged to the Nairovirus genus, Bunyaviridae and is related to Tamdy virus (TAMV) that is also associated with the ixodidae ticks of pasture biocenosis in Central Asia. Previous studies showed that TAMV is the prototypic virus of new phylogenetic Tamdy group in the Nairovirus genus. Thus, BURV was classified as a new virus of the Tamdy group, Nairovirus, Bunyaviridae. PMID:25549462

  8. Sequence diversity of the nucleoprotein gene of iris yellow spot virus (genus Tospovirus, family Bunyaviridae) isolates from the western region of the United States.

    Science.gov (United States)

    Pappu, H R; du Toit, L J; Schwartz, H F; Mohan, S K

    2006-05-01

    Iris yellow spot virus (IYSV), a tentative virus species in the genus Tospovirus and family Bunyaviridae, is considered a rapidly emerging threat to onion production in the western United States (US). The present study was undertaken to determine the sequence diversity of IYSV isolates from infected onion plants grown in California, Colorado, Idaho, Oregon, Utah and Washington. Using primers derived from the small RNA of IYSV, the complete sequence of the nucleoprotein (NP) gene of each isolate was determined and the sequences compared. In addition, a shallot isolate of IYSV from Washington was included in the study. The US isolates of IYSV shared a high degree of sequence identity (95 to 99%) with one another and to previously reported isolates. Phylogenetic analyses showed that with the exception of one isolate from central Oregon and one isolate from California, all the onion and shallot isolates from the western US clustered together. This cluster also included onion and lisianthus isolates from Japan. A second distinct cluster consisted of isolates from Australia (onion), Brazil (onion), Israel (lisianthus), Japan (alstroemeria), The Netherlands (iris) and Slovenia (leek). The IYSV isolates evaluated in this study appear to represent two distinct groups, one of which largely represents isolates from the western US. Understanding of the population structure of IYSV would potentially provide insights into the molecular epidemiology of this virus. PMID:16320007

  9. Uso de células de Aedes albopictus C6/36 na propagação e classificação de arbovírus das famílias Togaviridae, Flaviviridae, Bunyaviridae e Rhabdoviridae

    OpenAIRE

    Luiz Tadeu Moraes Figueiredo

    1990-01-01

    Colônias de células de mosquito Aedes albopictus C6/36 foram infectadas com 23 arbovirus, sendo 19 destes existentes no Brasil, pertencentes às famílias Togaviridae, Flaviviridae, Bunyaviridae e Rhabdoviridae. A Replicação virai foi detectada por imunofluorescência indireta com todos os vírus estudados enquanto que o efeito citopático foi observado durante a infecção por alguns destes. No teste de imunofluorescência indireta utilizou-se fluidos ascíticos imunes de camundongos, específicos par...

  10. Caracterização e relacionamento antigênico de três novos Bunyavirus no grupo Anopheles A (Bunyaviridae dos arbovirus

    Directory of Open Access Journals (Sweden)

    Rosa Jorge Fernando Soares Travassos da

    1992-01-01

    Full Text Available São descritos o isolamento e a caracterização de três novos arbovirus isolados na região da Usina Hidro-Elétrica de Tucuruí (UHE-TUC. Os três novos arbovirus pertencem ao grupo Anopheles A(ANA, gênero Bunyavirus (família Bunyaviridae. Os vírus Tucuruí (TUC, Caraipé (CPE e Arumateua (ART são relacionados entre si e com o vírus Trombetas (TBT, formando dentro do grupo ANA um complexo chamado Trombetas. Os arbovirus TUC, CPE e ART foram obtidos a partir de lotes de mosquitos Anopheles (Nyssorhynchus sp capturados em Tucuruí, nas proximidades da usina hidrelétrica de Tucuruí, Estado do Pará, nos meses de fevereiro, agosto e outubro de 1984, respectivamente. Até o final de 1990 os vírus TUC, CPE e ART foram isolados 12, 32 e 28 vezes respectivamente, sempre na região da UHE-TUC, exceção feita ao vírus TUC, do qual se obteve uma amostra procedente de Balbina, onde também foi construída uma hidroelétrica. Até o presente, esses vírus só foram isolados a partir de mosquitos do grupo An. (Nys. principalmente, a partir das espécies An. (Nys. nuneztovari e An. (Nys. triannulatus também consideradas vetores secundários da malária na Amazônia Brasileira. Testes sorológicos executados com soros humanos e de diversas espécies de animais silvestres foram negativos, com exceção de um soro de um carnívoro de espécie Nasua nasua que neutralizou a amostra TUC em títulos de 2.6 índice logaritmico de neutralização (ILN.

  11. Caracterização e relacionamento antigênico de três novos Bunyavirus no grupo Anopheles A (Bunyaviridae dos arbovirus

    Directory of Open Access Journals (Sweden)

    Jorge Fernando Soares Travassos da Rosa

    1992-06-01

    Full Text Available São descritos o isolamento e a caracterização de três novos arbovirus isolados na região da Usina Hidro-Elétrica de Tucuruí (UHE-TUC. Os três novos arbovirus pertencem ao grupo Anopheles A(ANA, gênero Bunyavirus (família Bunyaviridae. Os vírus Tucuruí (TUC, Caraipé (CPE e Arumateua (ART são relacionados entre si e com o vírus Trombetas (TBT, formando dentro do grupo ANA um complexo chamado Trombetas. Os arbovirus TUC, CPE e ART foram obtidos a partir de lotes de mosquitos Anopheles (Nyssorhynchus sp capturados em Tucuruí, nas proximidades da usina hidrelétrica de Tucuruí, Estado do Pará, nos meses de fevereiro, agosto e outubro de 1984, respectivamente. Até o final de 1990 os vírus TUC, CPE e ART foram isolados 12, 32 e 28 vezes respectivamente, sempre na região da UHE-TUC, exceção feita ao vírus TUC, do qual se obteve uma amostra procedente de Balbina, onde também foi construída uma hidroelétrica. Até o presente, esses vírus só foram isolados a partir de mosquitos do grupo An. (Nys. principalmente, a partir das espécies An. (Nys. nuneztovari e An. (Nys. triannulatus também consideradas vetores secundários da malária na Amazônia Brasileira. Testes sorológicos executados com soros humanos e de diversas espécies de animais silvestres foram negativos, com exceção de um soro de um carnívoro de espécie Nasua nasua que neutralizou a amostra TUC em títulos de 2.6 índice logaritmico de neutralização (ILN.

  12. Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae transmitted virus Mancha clorótica do Clerodendrum, uma enfermidade causada por um vírus do tipo nuclear, transmitido pelo ácaro Brevipalpus phoenicis (Acari:Tenuipalpidae

    Directory of Open Access Journals (Sweden)

    Elliot Watanabe Kitajima

    2008-02-01

    Full Text Available Chlorotic spots have been observed in plants of Clerodendrum x speciosum growing in residential gardens and parks in Piracicaba, SP, Brazil. Thin sections of diseased tissues revealed characteristic cytopathic effects of the nuclear type of the Brevipalpus (Acari: Tenuipalpidae mite-transmitted viruses (BTrV. Brevipalpus mites, identified as B. phoenicis, infesting symptomatic C. x speciosum plants transmitted the pathogen to healthy C. x speciosum and to C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi and Tetragonia expansa causing chlorotic spots on their leaves. Mechanical inoculation using leaf extracts from infected C. x speciosum resulted in chlorotic spots on inoculated C. x speciosum, Chenopodium quinoa, C. amaranticolor, G. globosa, H. cannabinus, H. coccineus and T. expansa leaves. C. amaranticolor and C. quinoa kept at 28 - 30°C became systemically infected. The same cytopathic effects caused by the nuclear type of BTrV were seen in tissues from all infected test plants by electron microscopy. The virus was purified from systemically infected leaves of C. amaranticolor and C. quinoa. A polyclonal antiserum obtained from an immunized rabbit presented a strong reaction with the homologous antigen in ELISA tests. The results suggest that this chlorotic spot disease of C. x speciosum is caused by a new species of the nuclear type of BTrV, tentatively named Clerodendrum chlorotic spot virus (ClCSV.Manchas cloróticas e necróticas foram observadas em folhas de várias plantas de coração-sangrento (Clerodendrum x speciosum cultivadas em parques e jardins em Piracicaba, SP, associadas à infestação pelo ácaro tenuipalpídeo Brevipalpus phoenicis. Exames preliminares de secções de tecido das manchas cloróticas ao microscópio eletrônico revelaram a ocorrência de efeitos citopáticos característicos dos induzidos pelos vírus do tipo nuclear, transmitido

  13. Caracterização de um vírus baciliforme isolado de Solanum violaefolium transmitido pelos ácaros Brevipalpus phoenicis e Brevipalpus obovatus (Acari: Tenuipalpidae Characterization of a bacilliform virus isolated from Solanum violaefolium transmitted by the tenuipalpid mites Brevipalpus phoenicis and Brevipalpus obovatus

    Directory of Open Access Journals (Sweden)

    Paulo de Tarso Oliveira Ferreira

    2007-09-01

    Full Text Available Solano-violeta (Solanum violaefolium é uma planta ornamental rasteira usada para cobrir solos de áreas sombreadas. Um vírus que induz manchas anelares nas folhas desta planta, tentativamente designado Solanum violaefolium ringspot virus - SvRSV, transmitido pelo ácaro Brevipalpus phoenicis (Acari: Tenuipalpidae foi encontrado em Piracicaba, SP. Trata-se de um vírus baciliforme que se assemelha a outros vírus do tipo citoplasmático transmitidos por Brevipalpus sp. Este trabalho teve como objetivo relatar propriedades biológicas e estabelecer uma caracterização molecular parcial do SvRSV. O vírus pode ser transmitido mecanicamente a várias outras espécies botânicas, causando lesões localizadas. Entre as espécies avaliadas, Datura stramonium mostrou-se a melhor hospedeira experimental. Observou-se também a manifestação de sintomas nestas plantas após infestação das mesmas por B. obovatus previamente alimentado em lesões de SvRSV, confirmando esta outra espécie de ácaro como vetor do vírus. Suas propriedades físicas in vitro foram: temperatura de inativação 40-45 ºC; ponto final de diluição 10-3-10-4; longevidade in vitro 12 dias. Em secções ultrafinas, as partículas do SvRSV mostraram-se levemente mais delgadas e mais longas que as de outros vírus do mesmo grupo. A partir do dsRNA do SvRSV foi construída uma biblioteca de cDNA e foram identificadas duas possíveis regiões codificadoras das proteínas de movimento e replicase viral. Baseado nestas regiões foram desenhados "primers" para amplificação do RNA do SvRSV por RT-PCR. Sondas baseadas nas seqüências obtidas hibridizaram com ss- e dsRNA de D. stramonium infectadas pelo vírus. Ensaios preliminares de RT-PCR e hibridização não resultaram em reação com o vírus da leprose dos citros, tipo citoplasmático (CiLV-C.Solanum violaefolium is an ornamental plant, with prostrate, trailing growth habit and is cultivated in shaded areas. A virus that causes

  14. Quantitative Real-Time PCR Detection of Rift Valley Fever Virus and Its Application to Evaluation of Antiviral Compounds

    OpenAIRE

    Garcia, Stephan; Crance, Jean Marc; Billecocq, Agnes; Peinnequin, Andre; Jouan, Alain; Bouloy, Michele; Garin, Daniel

    2001-01-01

    The Rift Valley fever virus (RVFV), a member of the genus Phlebovirus (family Bunyaviridae) is an enveloped negative-strand RNA virus with a tripartite genome. Until 2000, RVFV circulation was limited to the African continent, but the recent deadly outbreak in the Arabian Peninsula dramatically illustrated the need for rapid diagnostic methods, effective treatments, and prophylaxis. A method for quantifying the small RNA segment by a real-time detection reverse transcription (RT)-PCR using Ta...

  15. Rift Valley Fever Virus Incorporates the 78 kDa Glycoprotein into Virions Matured in Mosquito C6/36 Cells

    OpenAIRE

    Weingartl, Hana M.; Zhang, Shunzhen; Marszal, Peter; McGreevy, Alan; Burton, Lynn; Wilson, William C.

    2014-01-01

    Rift Valley fever virus (RVFV), genus Phlebovirus, family Bunyaviridae is a zoonotic arthropod-borne virus able to transition between distant host species, causing potentially severe disease in humans and ruminants. Viral proteins are encoded by three genomic segments, with the medium M segment coding for four proteins: nonstructural NSm protein, two glycoproteins Gn and Gc and large 78 kDa glycoprotein (LGp) of unknown function. Goat anti-RVFV polyclonal antibody and mouse monoclonal antibod...

  16. Partial genetic characterization of Sedlec virus (Orthobunyavirus, Bunyaviridae)

    Czech Academy of Sciences Publication Activity Database

    Bakonyi, T.; Kolodziejek, J.; Rudolf, Ivo; Berčič, R.; Nowotny, N.; Hubálek, Zdeněk

    2013-01-01

    Roč. 19, October (2013), s. 244-249. ISSN 1567-1348 Institutional support: RVO:68081766 Keywords : Sedlec virus * Leanyer virus * Simbu group * Orthobunyavirus * Acrocephalus Subject RIV: EE - Microbiology, Virology Impact factor: 3.264, year: 2013

  17. Biogeography of tick-borne Bhanja virus (Bunyaviridae) in Europe

    Czech Academy of Sciences Publication Activity Database

    Hubálek, Zdeněk

    2009-01-01

    Roč. 2009, č. 372691 (2009), s. 1-11. ISSN 1687-708X Grant ostatní: 6th Framework Programme(XE) GOCE-2003-010284 EDEN Institutional research plan: CEZ:AV0Z60930519 Keywords : Bhanja virus * biogeography * arbovirus es Subject RIV: EE - Microbiology, Virology

  18. Single-particle cryo-electron microscopy of Rift Valley fever virus

    International Nuclear Information System (INIS)

    Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T = 12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines.

  19. The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses.

    Science.gov (United States)

    Agirrezabala, Xabier; Méndez-López, Eduardo; Lasso, Gorka; Sánchez-Pina, M Amelia; Aranda, Miguel; Valle, Mikel

    2015-01-01

    Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We describe here a structure at 3.9 Å resolution, from electron cryomicroscopy, of Pepino mosaic virus (PepMV), a representative of the genus Potexvirus (family Alphaflexiviridae). Our results allow modeling of the CP and its interactions with viral RNA. The overall fold of PepMV CP resembles that of nucleoproteins (NPs) from the genus Phlebovirus (family Bunyaviridae), a group of enveloped (-)ssRNA viruses. The main difference between potexvirus CP and phlebovirus NP is in their C-terminal extensions, which appear to determine the characteristics of the distinct multimeric assemblies - a flexuous, helical rod or a loose ribonucleoprotein. The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses. PMID:26673077

  20. Presence of Viral RNA and Proteins in Exosomes from Cellular Clones Resistant to Rift Valley Fever Virus Infection

    OpenAIRE

    Ahsan, Noor A.; Sampey, Gavin C.; Lepene, Ben; Akpamagbo, Yao; Barclay, Robert A.; Iordanskiy, Sergey; Hakami, Ramin M.; KASHANCHI, FATAH

    2016-01-01

    Rift Valley Fever Virus (RVFV) is a RNA virus that belongs to the genus Phlebovirus, family Bunyaviridae. It infects humans and livestock and causes Rift Valley fever. RVFV is considered an agricultural pathogen by the USDA, as it can cause up to 100% abortion in cattle and extensive death of newborns. In addition, it is designated as Category A pathogen by the CDC and the NIAID. In some human cases of RVFV infection, the virus causes fever, ocular damage, liver damage, hemorrhagic fever, and...

  1. Proteomics computational analyses suggest that the carboxyl terminal glycoproteins of Bunyaviruses are class II viral fusion protein (beta-penetrenes

    Directory of Open Access Journals (Sweden)

    Garry Robert F

    2004-11-01

    Full Text Available Abstract The Bunyaviridae family of enveloped RNA viruses includes five genuses, orthobunyaviruses, hantaviruses, phleboviruses, nairoviruses and tospoviruses. It has not been determined which Bunyavirus protein mediates virion:cell membrane fusion. Class II viral fusion proteins (beta-penetrenes, encoded by members of the Alphaviridae and Flaviviridae, are comprised of three antiparallel beta sheet domains with an internal fusion peptide located at the end of domain II. Proteomics computational analyses indicate that the carboxyl terminal glycoprotein (Gc encoded by Sandfly fever virus (SAN, a phlebovirus, has a significant amino acid sequence similarity with envelope protein 1 (E1, the class II fusion protein of Sindbis virus (SIN, an Alphavirus. Similar sequences and common structural/functional motifs, including domains with a high propensity to interface with bilayer membranes, are located collinearly in SAN Gc and SIN E1. Gc encoded by members of each Bunyavirus genus share several sequence and structural motifs. These results suggest that Gc of Bunyaviridae, and similar proteins of Tenuiviruses and a group of Caenorhabditis elegans retroviruses, are class II viral fusion proteins. Comparisons of divergent viral fusion proteins can reveal features essential for virion:cell fusion, and suggest drug and vaccine strategies.

  2. Estudo de hemoparasitas transmitidos por vectores, em cães de canil, Setúbal, Portugal

    OpenAIRE

    Silva, Inês Picanço Castanheira da

    2011-01-01

    As doenças transmitidas por vectores são causadas por parasitas, bactérias ou vírus e são veiculadas pela picada de artrópodes (principalmente ixodídeos e mosquitos). Estas doenças afectam cães a nível mundial e a maioria delas têm potencial zoonótico. Nestas estão incluídas as doenças sob estudo: Erliquiose, Babesiose, Riquetsiose, Anaplasmose e Leishmaniose. A patogénese das doenças sob estudo, baseia-se fundamentalmente na resposta humoral exagerada e não protectora desenvolvida pelo ho...

  3. Valores, contravalores y sentimientos, transmitidos por los medios de comunicación y su percepción reflexiva

    OpenAIRE

    Bartolomé Crespo, Donaciano

    1997-01-01

    Esta investigación se refiere a la percepción de valores, antivalores y sentimientos encarnados y manifiestos en imágenes, palabras y sonidos autopresentados o mediados. Si en el pasado se han estudiado preferentemente los medios y sus mensajes, la preocupación actual mayoritaria sería investigar lo que pasa dentro del sujeto, cómo reacciona, cuáles son sus sentimientos. No hay duda que la reflexión sobre valores y propios sentimientos que suscitan la escucha, visionado o lectura de...

  4. Valores, Contravalores y Sentimientos, transmitidos por los medios de comunicación y su percepción reflexiva

    OpenAIRE

    Bartolomé Crespo, Donaciano

    1997-01-01

    This research deals with the perception of values, antivalues and feelings incarnated and manifested in images, words and sounds self introduced or mediated. If in the past media and their messages have been studied, the current concern of the majority would be to investigate what happens inside the individual, how he or she reacts, what are his /her feelings. There is no doubt that the reflection about values an own feelings that listening, looking or reading news create, is a very attractiv...

  5. Emergence of a new lineage of Cache Valley virus (Bunyaviridae: Orthobunyavirus) in the Northeastern United States.

    Science.gov (United States)

    Armstrong, Philip M; Andreadis, Theodore G; Anderson, John F

    2015-07-01

    Cache Valley virus (CVV; Family Bunyavidae, Genus Orthobunyavirus) is a mosquito-borne zoonosis that frequently infects humans and livestock in North and Central America. In the northeastern United States, CVV transmission is unpredictable from year-to-year and may derive from the periodic extinction and reintroduction of new virus strains into this region. To evaluate this possibility, we sequenced and analyzed numerous CVV isolates sampled in Connecticut during an 18-year period to determine how the virus population may change over time. Phylogenetic analyses showed the establishment of a new viral lineage during 2010 that became dominant by 2014 and appears to have originated from southern Mexico. CVV strains from Connecticut also grouped into numerous sub-clades within each lineage that included viruses from other U.S. states and Canada. We did not observe the development and stable persistence of local viral clades in Connecticut, which may reflect the episodic pattern of CVV transmission. Together, our data support the emergence of a new lineage of CVV in the northeastern United States and suggest extensive dispersal of viral strains in North America. PMID:25962774

  6. Structure of the Rift Valley fever virus nucleocapsid protein reveals another architecture for RNA encapsidation

    Energy Technology Data Exchange (ETDEWEB)

    Raymond, Donald D.; Piper, Mary E.; Gerrard, Sonja R.; Smith, Janet L. (Michigan)

    2010-07-13

    Rift Valley fever virus (RVFV) is a negative-sense RNA virus (genus Phlebovirus, family Bunyaviridae) that infects livestock and humans and is endemic to sub-Saharan Africa. Like all negative-sense viruses, the segmented RNA genome of RVFV is encapsidated by a nucleocapsid protein (N). The 1.93-{angstrom} crystal structure of RVFV N and electron micrographs of ribonucleoprotein (RNP) reveal an encapsidated genome of substantially different organization than in other negative-sense RNA virus families. The RNP polymer, viewed in electron micrographs of both virus RNP and RNP reconstituted from purified N with a defined RNA, has an extended structure without helical symmetry. N-RNA species of {approx}100-kDa apparent molecular weight and heterogeneous composition were obtained by exhaustive ribonuclease treatment of virus RNP, by recombinant expression of N, and by reconstitution from purified N and an RNA oligomer. RNA-free N, obtained by denaturation and refolding, has a novel all-helical fold that is compact and well ordered at both the N and C termini. Unlike N of other negative-sense RNA viruses, RVFV N has no positively charged surface cleft for RNA binding and no protruding termini or loops to stabilize a defined N-RNA oligomer or RNP helix. A potential protein interaction site was identified in a conserved hydrophobic pocket. The nonhelical appearance of phlebovirus RNP, the heterogeneous {approx}100-kDa N-RNA multimer, and the N fold differ substantially from the RNP and N of other negative-sense RNA virus families and provide valuable insights into the structure of the encapsidated phlebovirus genome.

  7. De los males transmitidos al tercer milenio el fracaso de las prisiones y la falsa seguridad ofrecida en colombia. las promesas incumplidas de la constitucion del 91

    OpenAIRE

    Martínez, Mauricio

    2013-01-01

    Este artículo formula una exposición clara sobre el problema penitenciario en Colombia desde dos perspectivas: la diacrónica, que da cuenta de la discusión teórica y académica que originó la ciencia criminológica contemporánea y la sincrónica, que explica las razones del fracaso histórico que ha representado para Colombia el paso a la justicia penal. Reúne además el estudio, elementos y daos de análisis cuantitativo en los que fundamenta el diagnóstico. Así mismo, presenta en su desarrollo ...

  8. The product category and the message informed in food children’s advertising / La categoría de producto y el mensaje transmitido

    OpenAIRE

    Lic. Cristina González Díaz [C. V.Lic. Cristina González Díaz

    2008-01-01

    This article explains the worry about children obesity and the accusation that many sectors exert on advertising. Furthermore, it tries to develop a theoric framework in order to set out the researches, which have been done, about food children’s advertising and its connection with obesity. In adittion to this, the article talks about other researches which prove the restricted influence advertising exerts on children as regards food consumption and the factors which are necessary to affirm...

  9. The product category and the message informed in food children’s advertising / La categoría de producto y el mensaje transmitido

    Directory of Open Access Journals (Sweden)

    Lic. Cristina González Díaz [C. V.Lic. Cristina González Díaz

    2008-01-01

    Full Text Available This article explains the worry about children obesity and the accusation that many sectors exert on advertising. Furthermore, it tries to develop a theoric framework in order to set out the researches, which have been done, about food children’s advertising and its connection with obesity. In adittion to this, the article talks about other researches which prove the restricted influence advertising exerts on children as regards food consumption and the factors which are necessary to affirm that there are direct causes to provoke obesity. The purpose of this essay is to analyze what type of product category exerts more advertising pressure and what kind of messages are used. This analysis will expound if children’s advertising is really surrounded by non healthy products or, however, by advisable ones in a child’s diet. Finally, it will also expound which communicative subjects are found in this kind of products.Resumen: El presente artículo, contextualizado bajo la preocupación de la obesidad infantil y la acusación que desde numerosos sectores se ejerce sobre la publicidad, ha pretendido desarrollar un marco teórico donde se expusieran las investigaciones que sobre el binomio publicidad infantil de alimentos – obesidad se han realizado; así como aquellas donde se dejaba evidencia de la limitada influencia que la misma podía realizar sobre el menor en lo concerniente a consumo de alimentos, y los numerosos factores necesarios para poder hablar de causantes directos de la problemática de la obesidad. El objetivo sobre el que subyace el trabajo trata de analizar los tipos de productos que más presión publicitaria ejercen así como los mensajes sobre los que versa su estrategia comunicativa para así poder observar si realmente la publicidad infantil está copada por productos poco saludables o recomendables en la dieta del menor y observar qué tipo de temática comunicativa impregna este tipo de productos.

  10. Evaluation of the eastern cottontail Sylvilagus floridanus as an amplifying vertebrate host for Cache Valley virus (Bunyaviridae) in Indiana.

    Science.gov (United States)

    Blackmore, Carina G M; Grimstad, Paul R

    2008-01-01

    To evaluate the importance of eastern cottontails (Sylvilagus floridanus) as amplifying hosts for Cache Valley virus (CVV), we tested hunter-provided blood samples from northern Indiana for specific neutralizing (N) antibodies against this mosquito-borne bunya-virus. Samples were collected during the winter of 1994-95. Two seronegative eastern cottontails, captured in July 1995, were also infected with CVV by subcutaneous inoculation, and two others were infected by allowing CVV-infected mosquitoes to feed on them. The results indicate that eastern cottontails probably are not important amplifying hosts for CVV. The prevalence of N antibodies against CVV was low (6.0%, n=82) among the hunter-killed animals. Low viremia (<1.8 log10 plaque-forming units/ml) of short duration (1-3 days) were seen in three of four experimentally infected eastern cottontails. The viremias were insufficient for infecting Coquillettidia perturbans, a mosquito species commonly found naturally infected with CVV. PMID:18263839

  11. Epidemiologic relationship between Toscana virus infection and Leishmania infantum due to common exposure to Phlebotomus perniciosus sandfly vector.

    Directory of Open Access Journals (Sweden)

    Laurence Bichaud

    2011-09-01

    Full Text Available Sand flies are recognised vectors of parasites in the genus Leishmania and a number of arthropod-borne viruses, in particular viruses within the genus Phlebovirus, family Bunyaviridae. In southern France, Toscana phlebovirus (TOSV is recognized as a prominent cause of summer meningitis. Since Leishmania and TOSV have a common vector (Phlebotomus perniciosus, an epidemiologic link has been assumed for a long time. However, there is no scientific evidence of such a link between human leishmaniosis and phleboviral infections. To identify a possible link, we investigated the presence and distribution of antibodies against these two microorganisms (i in individuals and (ii at a spatial level in the city of Marseille (south-eastern France. Five hundred sera were selected randomly in the biobank of the Department of Parasitology of the Public Hospitals of Marseille. All sera were previously tested for IgG against Leishmania by Western Blotting, and TOSV IgG were detected by indirect immunofluorescence. The seropositivity rates were 21.4% for TOSV and 28% for Leishmania. Statistical analysis demonstrated that seropositivity for one pathogen was significantly associated with seropositivity to the other pathogen. This result provided the first robust evidence for the existence of an epidemiological relationship between Leishmania infantum and TOSV. Addresses of tested patients were geolocalized and integrated into Geographical Information System software, in order to test spatial relationship between the two pathogens. Spatial analysis did not allow to identify (i specific patterns for the spatial distribution of positive serological results for TOSV or Leishmania, and (ii a spatial relationship between Leishmania and TOSV positive serological results. This may reflect the fact that the sample studied was not powerful enough to demonstrate either a spatial clustering or co-location, i.e. that the actual risk exposure area is smaller than the mean of

  12. The genome sequence of Lone Star virus, a highly divergent bunyavirus found in the Amblyomma americanum tick.

    Science.gov (United States)

    Swei, Andrea; Russell, Brandy J; Naccache, Samia N; Kabre, Beniwende; Veeraraghavan, Narayanan; Pilgard, Mark A; Johnson, Barbara J B; Chiu, Charles Y

    2013-01-01

    Viruses in the family Bunyaviridae infect a wide range of plant, insect, and animal hosts. Tick-borne bunyaviruses in the Phlebovirus genus, including Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) in China, Heartland virus (HRTV) in the United States, and Bhanja virus in Eurasia and Africa have been associated with acute febrile illness in humans. Here we sought to characterize the growth characteristics and genome of Lone Star virus (LSV), an unclassified bunyavirus originally isolated from the lone star tick Amblyomma americanum. LSV was able to infect both human (HeLa) and monkey (Vero) cells. Cytopathic effects were seen within 72 h in both cell lines; vacuolization was observed in infected Vero, but not HeLa, cells. Viral culture supernatants were examined by unbiased deep sequencing and analysis using an in-house developed rapid computational pipeline for viral discovery, which definitively identified LSV as a phlebovirus. De novo assembly of the full genome revealed that LSV is highly divergent, sharing identity with any other bunyavirus. Despite this sequence diversity, LSV was found by phylogenetic analysis to be part of a well-supported clade that includes members of the Bhanja group viruses, which are most closely related to SFSTV/HRTV. The genome sequencing of LSV is a critical first step in developing diagnostic tools to determine the risk of arbovirus transmission by A. americanum, a tick of growing importance given its expanding geographic range and competence as a disease vector. This study also underscores the power of deep sequencing analysis in rapidly identifying and sequencing the genomes of viruses of potential clinical and public health significance. PMID:23637969

  13. Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.

    Directory of Open Access Journals (Sweden)

    Nathan D Grubaugh

    Full Text Available BACKGROUND: Arthropod-borne viruses are important emerging pathogens world-wide. Viruses transmitted by mosquitoes, such as dengue, yellow fever, and Japanese encephalitis viruses, infect hundreds of millions of people and animals each year. Global surveillance of these viruses in mosquito vectors using molecular based assays is critical for prevention and control of the associated diseases. Here, we report an oligonucleotide DNA microarray design, termed ArboChip5.1, for multi-gene detection and identification of mosquito-borne RNA viruses from the genera Flavivirus (family Flaviviridae, Alphavirus (Togaviridae, Orthobunyavirus (Bunyaviridae, and Phlebovirus (Bunyaviridae. METHODOLOGY/PRINCIPAL FINDINGS: The assay utilizes targeted PCR amplification of three genes from each virus genus for electrochemical detection on a portable, field-tested microarray platform. Fifty-two viruses propagated in cell-culture were used to evaluate the specificity of the PCR primer sets and the ArboChip5.1 microarray capture probes. The microarray detected all of the tested viruses and differentiated between many closely related viruses such as members of the dengue, Japanese encephalitis, and Semliki Forest virus clades. Laboratory infected mosquitoes were used to simulate field samples and to determine the limits of detection. Additionally, we identified dengue virus type 3, Japanese encephalitis virus, Tembusu virus, Culex flavivirus, and a Quang Binh-like virus from mosquitoes collected in Thailand in 2011 and 2012. CONCLUSIONS/SIGNIFICANCE: We demonstrated that the described assay can be utilized in a comprehensive field surveillance program by the broad-range amplification and specific identification of arboviruses from infected mosquitoes. Furthermore, the microarray platform can be deployed in the field and viral RNA extraction to data analysis can occur in as little as 12 h. The information derived from the ArboChip5.1 microarray can help to establish

  14. Risk assessment of human infection with a novel bunyavirus in China

    Directory of Open Access Journals (Sweden)

    Tamano Matsui

    2012-11-01

    Full Text Available Objective: To assess the public health risk of human infection from a novel bunyavirus – severe fever with thrombocytopenia syndrome virus (SFTSV – in China.Methods: The likelihood of disease spread and the magnitude of public health impact were assessed to clarify overall risk. Literature about hazard, exposure and contextual factors associated with SFTSV infection was collected and reviewed. Information on SFTSV cases and the population in six provinces under surveillance was compared.Results: SFTSV is a member of the Phlebovirus genus of the Bunyaviridae family. A widely distributed tick species, Haemaphysalis longicornis, can act as the vector; thus the disease is likely to spread in China. Symptoms of SFTSV infection are nonspecific, but have led to multiorgan dysfunction in severe cases. High-risk populations include farmers and older females. Evidence of human-to-human transmission within family and hospital has been reported. The capacity for treatment and diagnosis of SFTSV are adequate in rural communities in China, and community awareness of the disease should be high. Discussion: There is a low to moderate public health risk related to SFTSV human infection in China. There is potential for an increase in the number of cases reported as awareness increases and when surveillance is expanded.

  15. Protein Phosphatase-1 regulates Rift Valley fever virus replication.

    Science.gov (United States)

    Baer, Alan; Shafagati, Nazly; Benedict, Ashwini; Ammosova, Tatiana; Ivanov, Andrey; Hakami, Ramin M; Terasaki, Kaori; Makino, Shinji; Nekhai, Sergei; Kehn-Hall, Kylene

    2016-03-01

    Rift Valley fever virus (RVFV), genus Phlebovirus family Bunyaviridae, is an arthropod-borne virus endemic throughout sub-Saharan Africa. Recent outbreaks have resulted in cyclic epidemics with an increasing geographic footprint, devastating both livestock and human populations. Despite being recognized as an emerging threat, relatively little is known about the virulence mechanisms and host interactions of RVFV. To date there are no FDA approved therapeutics or vaccines for RVF and there is an urgent need for their development. The Ser/Thr protein phosphatase 1 (PP1) has previously been shown to play a significant role in the replication of several viruses. Here we demonstrate for the first time that PP1 plays a prominent role in RVFV replication early on during the viral life cycle. Both siRNA knockdown of PP1α and a novel PP1-targeting small molecule compound 1E7-03, resulted in decreased viral titers across several cell lines. Deregulation of PP1 was found to inhibit viral RNA production, potentially through the disruption of viral RNA transcript/protein interactions, and indicates a potential link between PP1α and the viral L polymerase and nucleoprotein. These results indicate that PP1 activity is important for RVFV replication early on during the viral life cycle and may prove an attractive therapeutic target. PMID:26801627

  16. Rift Valley fever virus incorporates the 78 kDa glycoprotein into virions matured in mosquito C6/36 cells.

    Science.gov (United States)

    Weingartl, Hana M; Zhang, Shunzhen; Marszal, Peter; McGreevy, Alan; Burton, Lynn; Wilson, William C

    2014-01-01

    Rift Valley fever virus (RVFV), genus Phlebovirus, family Bunyaviridae is a zoonotic arthropod-borne virus able to transition between distant host species, causing potentially severe disease in humans and ruminants. Viral proteins are encoded by three genomic segments, with the medium M segment coding for four proteins: nonstructural NSm protein, two glycoproteins Gn and Gc and large 78 kDa glycoprotein (LGp) of unknown function. Goat anti-RVFV polyclonal antibody and mouse monoclonal antibody, generated against a polypeptide unique to the LGp within the RVFV proteome, detected this protein in gradient purified RVFV ZH501 virions harvested from mosquito C6/36 cells but not in virions harvested from the mammalian Vero E6 cells. The incorporation of LGp into the mosquito cell line - matured virions was confirmed by immune-electron microscopy. The LGp was incorporated into the virions immediately during the first passage in C6/36 cells of Vero E6 derived virus. Our data indicate that LGp is a structural protein in C6/36 mosquito cell generated virions. The protein may aid the transmission from the mosquitoes to the ruminant host, with a possible role in replication of RVFV in the mosquito host. To our knowledge, this is a first report of different protein composition between virions formed in insect C6/36 versus mammalian Vero E6 cells. PMID:24489907

  17. Rift Valley fever virus incorporates the 78 kDa glycoprotein into virions matured in mosquito C6/36 cells.

    Directory of Open Access Journals (Sweden)

    Hana M Weingartl

    Full Text Available Rift Valley fever virus (RVFV, genus Phlebovirus, family Bunyaviridae is a zoonotic arthropod-borne virus able to transition between distant host species, causing potentially severe disease in humans and ruminants. Viral proteins are encoded by three genomic segments, with the medium M segment coding for four proteins: nonstructural NSm protein, two glycoproteins Gn and Gc and large 78 kDa glycoprotein (LGp of unknown function. Goat anti-RVFV polyclonal antibody and mouse monoclonal antibody, generated against a polypeptide unique to the LGp within the RVFV proteome, detected this protein in gradient purified RVFV ZH501 virions harvested from mosquito C6/36 cells but not in virions harvested from the mammalian Vero E6 cells. The incorporation of LGp into the mosquito cell line - matured virions was confirmed by immune-electron microscopy. The LGp was incorporated into the virions immediately during the first passage in C6/36 cells of Vero E6 derived virus. Our data indicate that LGp is a structural protein in C6/36 mosquito cell generated virions. The protein may aid the transmission from the mosquitoes to the ruminant host, with a possible role in replication of RVFV in the mosquito host. To our knowledge, this is a first report of different protein composition between virions formed in insect C6/36 versus mammalian Vero E6 cells.

  18. Nucleotide variability of Ťahyňa virus (Bunyaviridae, Orthobunyavirus) small (S) and medium (M) genomic segments in field strains differing in biological properties

    Czech Academy of Sciences Publication Activity Database

    Kilian, P.; Růžek, Daniel; Danielová, V.; Hypša, Václav; Grubhoffer, Libor

    2010-01-01

    Roč. 149, č. 1 (2010), s. 119-123. ISSN 0168-1702 R&D Projects: GA MŠk(CZ) LC06009 Institutional research plan: CEZ:AV0Z60220518 Keywords : Tahyna virus * Bunyavirus * california group * genetic variability * virulence Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.905, year: 2010

  19. Evidencia serológica de infección por hantavirus (Bunyaviridae: Hantavirus) en roedores del Departamento de Sucre, Colombia

    OpenAIRE

    Pedro Blanco; Arroyo Stiven; Homer Corrales; Julia Pérez; Lercy Álvarez; Anaís Castellar

    2012-01-01

    Objetivo Determinar la frecuencia de anticuerpos específicos a hantavirus en roedores del municipio de San Marcos, departamento de Sucre. Métodos Se capturaron 144 roedores con trampas Sherman® en áreas urbanas y rurales del municipio de San Marcos, desde diciembre de 2007 hasta julio de 2009. Los anticuerpos Ig G específicos contra el Virus Sin Nombre (VSN) fueron detectados en muestras de plasma mediante ELISA indirecto. Resultados La seroprevalencia de anticuerpos contra hantavirus fue de...

  20. A theoretical model of the evolution of virulence in sexually transmitted HIV/AIDS Modelo teórico da evolucão da virulência do HIV/AIDS transmitido sexualmente

    Directory of Open Access Journals (Sweden)

    FAB Coutinho

    1999-08-01

    Full Text Available INTRODUCTION: The evolution of virulence in host-parasite relationships has been the subject of several publications. In the case of HIV virulence, some authors suggest that the evolution of HIV virulence correlates with the rate of acquisition of new sexual partners. In contrast some other authors argue that the level of HIV virulence is independent of the sexual activity of the host population. METHODS: Provide a mathematical model for the study of the potential influence of human sexual behaviour on the evolution of virulence of HIV is provided. RESULTS: The results indicated that, when the probability of acquisition of infection is a function both of the sexual activity and of the virulence level of HIV strains, the evolution of HIV virulence correlates positively with the rate of acquisition of new sexual partners. CONCLUSION: It is concluded that in the case of a host population with a low (high rate of exchange of sexual partners the evolution of HIV virulence is such that the less (more virulent strain prevails.INTRODUÇÃO: A evolução da virulência na relação hospedeiro-parasita tem sido objeto de várias publicações. No caso do HIV, alguns autores sugerem que a evolução da virulência do HIV correlaciona-se com a taxa de aquisição de novos parceiros sexuais. Por outro lado, outros autores argumentam que o nível de virulência do HIV é independente da atividade sexual da população hospedeira. MÉTODOS: Propõe-se um modelo matemático para estudar a influência potencial que o comportamento sexual humano possa ter na evolução da virulência do HIV. RESULTADOS: Os resultados indicam que, quando a probabilidade de aquisição da infecção pelo HIV é uma função tanto da atividade sexual da população humana quanto da virulência das cepas de HIV, a evolução da virulência do HIV correlaciona-se positivamente com a taxa de aquisição de novos parceiros sexuais. CONCLUSÃO: Concluiu-se que no caso de uma população hospedeira com uma baixa (alta taxa de troca de parceiros sexuais a evolução da virulência do HIV é tal que a cepa menos (mais virulenta predomina.

  1. Anatomia de lesões foliares causadas pelo vírus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies

    OpenAIRE

    Renata Takassugui Gomes; Elliot Watanabe Kitajima; Francisco André Osamu Tanaka; João Paulo Rodrigues Marques; Beatriz Appezzato-da-Glória

    2010-01-01

    O gênero botânico Clerodendrum pertence à família Lamiaceae e compreende várias espécies ornamentais, Manchas cloróticas e necróticas em folhas de coração-sangrento foram observadas pela primeira vez em um jardim de Piracicaba, SP, associadas à infestação com Brevipalpus phoenicis (Acari: Tenuipalpidae). Exames de secções de tecidos das lesões foliares ao microscópio eletrônico revelaram ocorrência de efeitos citopáticos do tipo nuclear e concluiu-se que os sintomas eram causados por um vírus...

  2. Molecular epidemiology of Rift Valley fever virus based on genetic analysis of the virus isolates recovered in 1944-2008 from distinct geographic regions

    International Nuclear Information System (INIS)

    Full text: Rift Valley fever (RVF) is an emerging mosquito-borne viral zoonosis caused by a RNA virus named Rift Valley fever virus (RVFV), a Phlebovirus member of the Bunyaviridae family. Historically the disease was present in Africa and Madagascar where outbreaks occur at irregular intervals when heavy rains facilitate the breeding of vector competent mosquito vectors. The occurrence of the first confirmed outbreaks of RVF in 2000-2001 among humans and livestock outside Africa, in the Arabian Peninsula, carries the implication of further spread of infection into non-endemic areas since the virus is capable of utilizing a wide range of mosquito vectors. This work undertook investigation of the molecular epidemiology of the disease (1944-2008) with special reference to South Africa where the first documented outbreak of RVF occurred in 1951 and the most recent in 2008. A total of 149 isolates of RVF recovered over a period of 65 years from various hosts and during endemic and epidemic periods of disease in 15 African countries, Madagascar and Saudi Arabia were characterised by partial genomic sequencing of a 535-nucleotide segment of the G2 glycoprotein coding region of the M segment and the genetic relatedness determined using MEGA software. Pair-wise comparison of RVF isolates revealed divergences ranging from 0-5.6% at the nucleotide level, corresponding to 0-2.8% at the amino acid level. Most isolates are compartmentalized geographically and belong to one of 16 genotypes within three main lineages. Isolates from South Africa collected over 57 years belong to one of 4 genotypes. The 2008 South African isolates were closely related to isolates from the recent east African outbreak in 2006 and a 2003 Mauritanian isolate. Phylogenetic analysis indicates that circulation of RVFV is highly compartmentalized but with favourable climatic conditions a single genotype can rapidly spread from endemic areas over vast distances to cause outbreaks in susceptible human and

  3. Planning for Rift Valley fever virus: use of geographical information systems to estimate the human health threat of white-tailed deer (Odocoileus virginianus-related transmission

    Directory of Open Access Journals (Sweden)

    Sravan Kakani

    2010-11-01

    Full Text Available Rift Valley fever (RVF virus is a mosquito-borne phlebovirus of the Bunyaviridae family that causes frequent outbreaks of severe animal and human disease in sub-Saharan Africa, Egypt and the Arabian Peninsula. Based on its many known competent vectors, its potential for transmission via aerosolization, and its progressive spread from East Africa to neighbouring regions, RVF is considered a high-priority, emerging health threat for humans, livestock and wildlife in all parts of the world. Introduction of West Nile virus to North America has shown the potential for “exotic” viral pathogens to become embedded in local ecological systems. While RVF is known to infect and amplify within domestic livestock, such as taurine cattle, sheep and goats, if RVF virus is accidentally or intentionally introduced into North America, an important unknown factor will be the role of local wildlife in the maintenance or propagation of virus transmission. We examined the potential impact of RVF transmission via white-tailed deer (Odocoileus virginianus in a typical north-eastern United States urban-suburban landscape, where livestock are rare but where these potentially susceptible, ungulate wildlife are highly abundant. Model results, based on overlap of mosquito, human and projected deer densities, indicate that a significant proportion (497/1186 km2, i.e. 42% of the urban and peri-urban landscape could be affected by RVF transmission during the late summer months. Deer population losses, either by intervention for herd reduction or by RVF-related mortality, would substantially reduce these likely transmission zones to 53.1 km2, i.e. by 89%.

  4. An unexpected recurrent transmission of Rift Valley fever virus in cattle in a temperate and mountainous area of Madagascar.

    Directory of Open Access Journals (Sweden)

    Veronique Chevalier

    2011-12-01

    Full Text Available Rift Valley fever is an acute, zoonotic viral disease of domestic ruminants, caused by a phlebovirus (Bunyaviridae family. A large outbreak occurred in Madagascar in 2008-2009. The goal of the present study was to evaluate the point prevalence of antibodies against Rift Valley Fever Virus (RVFV in cattle in the Anjozorobe district, located in the wet and temperate highland region of Madagascar and yet heavily affected by the disease, and analyse environmental and trade factors potentially linked to RVFV transmission. A serological study was performed in 2009 in 894 bovines. For each bovine, the following variables were recorded: age, location of the night pen, minimum distance from the pen to the nearest water point and the forest, nearest water point type, and herd replacement practices. The serological data were analyzed using a generalized linear mixed model. The overall anti-RVFV IgG seroprevalence rate was 28% [CI95% 25-31]. Age was statistically linked to prevalence (p = 10(-4, being consistent with a recurrent RVFV circulation. Distance from the night pen to the nearest water point was a protective factor (p = 5.10(-3, which would be compatible with a substantial part of the virus transmission being carried out by nocturnal mosquito vectors. However, water point type did not influence the risk of infection: several mosquito species are probably involved. Cattle belonging to owners who purchase animals to renew the herd were significantly more likely to have seroconverted than others (p = 0.04: cattle trade may contribute to the introduction of the virus in this area. The minimum distance of the night pen to the forest was not linked to the prevalence. This is the first evidence of a recurrent transmission of RVFV in such an ecosystem that associates a wet, temperate climate, high altitude, paddy fields, and vicinity to a dense rain forest. Persistence mechanisms need to be further investigated.

  5. [Taxonomy of previously unclassified Tamdy virus (TAMV) (Bunyaviridae, Nairovirus) isolated from the Hyalomma asiaticum asiaticum Schülce et Schlottke, 1929 (Ixodidae, Hyalomminae) in the Middle East and transcaucasia].

    Science.gov (United States)

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Aristova, V A; Gitel'man, A K; Deriabin, P G; Botikov, A G

    2014-01-01

    Complete genome sequencing of three Tamdy (TAMV) virus strains was carried out. The prototype strain TAMV/LEIV-1308Uz was isolated for the very first time from the Hyalomma asiaticum asiaticum Schülce et Schlottke, 1929 (Ixodidae, Hyalomminae) collected in the August 1971 from sheep in the arid area near Namdybulak town (41 degrees 36' N, 64 degrees 39' E) in the Tamdinsky district of the Bukhara region (Uzbekistan). TAMV was revealed to be a prototype member of the new phylogenetic group within the limits of the Nairovirus. The TAMV homology for RdRp (L-segment) amino acid sequence is not less than 40% with Crimea-Congo hemorrhagic fever virus (CCHFV), Hazara virus (HAZV), and Dugbe virus (DUGV), which are also linked with Ixodidae ticks. The TAMV homologies with the Issyk-Kul virus (ISKV) and Caspiy virus (CASV) for RdRp are 37.6% and 37.7%, respectively. These data conformed to the low values of GnGc (M-segment) and nucleocapsid protein N (S-segment) homology. The TAMV homologies with the nairoviruses for GnGc is in average 25%; with the nairoviruses linked with Ixodidae ticks (CCHFV, DUGV, HAZV) - 33%; with Argasidae ticks (ISKV, CASV) - 28%. The TAMV/LEIV-1308Uz, LEIV-6158Ar, and LEIV-10226Az have high level of identity. The TAMV/LEIV-10226Az from Azerbaijan has 99% homology for both nucleotide and amino acid sequences of the prototype TAMV/LEIV-1308Uz RdRp. The TAMV/LEIV-6158Ar from Armenia is more divergent and has 94.2% and 96.3% homologies with the TAMV/LEIV-1308Uz, respectively. The homology between the TAMV/LEIV-1308Uz and TAMV/LEIV-10226Az for GnGc is 93%. The TAMV/LEIV-6158Ar has 90% homology for this protein with the TAMV/LEIV-1308Uz and 93% with the TAMV/LEIV-10226Az, respectively. Differences in nucleocapsid protein between three TAMV strains are 5-7%. PMID:25069280

  6. Epidemiological study of Rift Valley fever virus in Kigoma, Tanzania

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    Emmanuel G. Kifaro

    2014-04-01

    Full Text Available Rift Valley fever virus (RVFV is an acute, zoonotic viral disease caused by a  Phlebovirus, which belongs to the Bunyaviridae family. Among livestock, outbreaks of the disease are economically devastating. They are often characterised by large, sweeping abortion storms and have significant mortality in adult livestock. The aim of the current study was to investigate RVFV infection in the Kigoma region, which is nestled under the hills of the western arm of the Great Rift Valley on the edge of Lake Tanganyika, Tanzania. A region-wide serosurvey was conducted on non-vaccinated small ruminants (sheep and goats, n = 411. Sera samples were tested for the presence of anti-RVFV antibodies and viral antigen, using commercial enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction, respectively. The overall past infections were detected in 22 of the 411 animals, 5.4% (Confidence Interval (CI 95% = 3.5% – 8.1%. The Kigoma rural area recorded the higher seroprevalence of 12.0% (CI 95% = 7.3% – 18.3%; p < 0.0001, followed by Kibondo at 2.3% (CI 95% = 0.5% – 6.5%; p > 0.05 and the Kasulu district at 0.8% (CI 95% = 0.0% – 4.2%; p > 0.05. The prevalence was 12.5% and 4.7% for sheep and goats, respectively. Reverse transcriptase polymerase chain reaction results indicated that only eight samples were found to be positive (n = 63. This study has confirmed, for the first time, the presence of the RVFV in the Kigoma region four years after the 2007 epizootic in Tanzania. The study further suggests that the virus activity exists during the inter-epizootic period, even in regions with no history of RVFV.

  7. Functional analysis of Rift Valley fever virus NSs encoding a partial truncation.

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    Jennifer A Head

    Full Text Available Rift Valley fever virus (RVFV, belongs to genus Phlebovirus of the family Bunyaviridae, causes high rates of abortion and fetal malformation in infected ruminants as well as causing neurological disorders, blindness, or lethal hemorrhagic fever in humans. RVFV is classified as a category A priority pathogen and a select agent in the U.S., and currently there are no therapeutics available for RVF patients. NSs protein, a major virulence factor of RVFV, inhibits host transcription including interferon (IFN-β mRNA synthesis and promotes degradation of dsRNA-dependent protein kinase (PKR. NSs self-associates at the C-terminus 17 aa., while NSs at aa.210-230 binds to Sin3A-associated protein (SAP30 to inhibit the activation of IFN-β promoter. Thus, we hypothesize that NSs function(s can be abolished by truncation of specific domains, and co-expression of nonfunctional NSs with intact NSs will result in the attenuation of NSs function by dominant-negative effect. Unexpectedly, we found that RVFV NSs truncated at aa. 6-30, 31-55, 56-80, 81-105, 106-130, 131-155, 156-180, 181-205, 206-230, 231-248 or 249-265 lack functions of IFN-β mRNA synthesis inhibition and degradation of PKR. Truncated NSs were less stable in infected cells, while nuclear localization was inhibited in NSs lacking either of aa.81-105, 106-130, 131-155, 156-180, 181-205, 206-230 or 231-248. Furthermore, none of truncated NSs had exhibited significant dominant-negative functions for NSs-mediated IFN-β suppression or PKR degradation upon co-expression in cells infected with RVFV. We also found that any of truncated NSs except for intact NSs does not interact with RVFV NSs even in the presence of intact C-terminus self-association domain. Our results suggest that conformational integrity of NSs is important for the stability, cellular localization and biological functions of RVFV NSs, and the co-expression of truncated NSs does not exhibit dominant-negative phenotype.

  8. Emerging and Reemeriging Human Bunyavirus Infections and Climate Change

    Science.gov (United States)

    Sutherland, Laura J.; Anyamba, Assaf; LaBeaud, A. Desiree

    2013-01-01

    The Bunyaviridae family includes a growing number of viruses that have contributed to the burden of emerging and reemerging infectious diseases around the globe. Many of these viruses cause severe clinical outcomes in human and animal populations, the results of which can be detrimental to public health and the economies of affected communities. The threat to endemic and non-native regions is particularly high, and national and international public health agencies are often on alert. Many of the bunyaviruses cause severe clinical disease including hemorrhage, organ failure, and death leading to their high-risk classification. Hantaviruses and Rift Valley fever virus (RVFV) (genus Phlebovirus) are National Institute of Allergy and Infectious Diseases Category A priority pathogens in the United States. Viral hemorrhagic fevers, a classification that includes many bunyaviruses, are immediately notifiable in the European Union. The emergence of new and reemerging bunyaviruses has resulted in numerous human and animal fatalities. Outbreaks of Rift Valley fever (RVF) in East Africa (1997/1998, 2006/2007), Sudan (2007), Southern Africa (2008-2010), Kenya (1997/1998, 2006/2007) (Anyamba et al., 2009, 2010; Breiman et al., 2010; Grobbelaar et al., 2011; Woods et al., 2002) and Saudi Arabia & Yemen (2000, 2010) (Food and Agriculture Organization, 2000; Hjelle and Glass, 2000; Madani et al., 2003) and the emergence of Sin Nombre virus (1993) (Hjelle and Glass, 2000) and most recently Schmallenberg virus (2011) (DEFRA, 2012) are prime examples of the devastating and worldwide toll bunyaviruses have on health and economies. Climate variability (precipitation and temperature in particular) greatly influence the ecological conditions that drive arboviral disease outbreaks across the globe. Several human and animal disease outbreaks have been influenced by changes in climate associated with the El Niño Southern Oscillation (ENSO) phenomenon including the bunyaviruses RVFV and Sin

  9. MP-12 virus containing the Clone 13 deletion in the NSs gene prevents lethal disease when administered after Rift Valley fever virus infection in hamsters

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    Brian B Gowen

    2015-06-01

    Full Text Available Rift Valley fever virus (RVFV; Bunyaviridae, Phlebovirus causes a range of illnesses that include retinitis, fulminant hepatitis, neurologic disease, and hemorrhagic fever. In hospitalized individuals, case fatality rates can be as high as 10-20%. There are no vaccines or antivirals approved for human use to prevent or treat severe RVFV infections. We previously tested the efficacy of the MP-12 vaccine strain and related variants with NSs truncations as a post-exposure prophylaxis in mice infected with wild-type pathogenic RVFV strain ZH501. Post-exposure efficacy of the rMP12-C13type, a recombinant MP-12 vaccine virus which encodes an in-frame truncation removing 69% of the NSs protein, resulted in 30% survival when administering the virus within 30 minutes of subcutaneous ZH501 challenge in mice, while the parental MP-12 virus conferred no protection by post-exposure vaccination. Here, we demonstrate uniform protection of hamsters by post-exposure vaccination with rMP12-C13type administered 6 h post-ZH501 infection while no efficacy was observed with the parental MP-12 virus. Notably, both the MP-12 and rMP12-C13type viruses were highly effective (100% protection when administered 21 days prior to challenge. In a subsequent study delaying vaccination until 8, 12 and 24 h post-RVFV exposure, we observed 80, 70 and 30% survival, respectively. Our findings indicate that the rapid protective innate immune response elicited by rMP12-C13type may be due to the truncated NSs protein, suggesting that the resulting functional inactivation of NSs plays an important role in the observed post-exposure efficacy. Taken together, the data demonstrate that post-exposure vaccination with rMP12-C13type is effective in limiting ZH501 replication and associated disease in standard pre-exposure vaccination and post-challenge treatment models of RVFV infection, and suggest an extended post-exposure prophylaxis window beyond that initially observed in mice.

  10. Role of Culex and Anopheles mosquito species as potential vectors of rift valley fever virus in Sudan outbreak, 2007

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    Galal Fatma H

    2010-03-01

    Full Text Available Abstract Background Rift Valley fever (RVF is an acute febrile arthropod-borne viral disease of man and animals caused by a member of the Phlebovirus genus, one of the five genera in the family Bunyaviridae. RVF virus (RVFV is transmitted between animals and human by mosquitoes, particularly those belonging to the Culex, Anopheles and Aedes genera. Methods Experiments were designed during RVF outbreak, 2007 in Sudan to provide an answer about many raised questions about the estimated role of vector in RVFV epidemiology. During this study, adult and immature mosquito species were collected from Khartoum and White Nile states, identified and species abundance was calculated. All samples were frozen individually for further virus detection. Total RNA was extracted from individual insects and RVF virus was detected from Culex, Anopheles and Aedes species using RT-PCR. In addition, data were collected about human cases up to November 24th, 2007 to asses the situation of the disease in affected states. Furthermore, a historical background of the RVF outbreaks was discussed in relation to global climatic anomalies and incriminated vector species. Results A total of 978 mosquitoes, belonging to 3 genera and 7 species, were collected during Sudan outbreak, 2007. Anopheles gambiae arabiensis was the most frequent species (80.7% in White Nile state. Meanwhile, Cx. pipiens complex was the most abundant species (91.2% in Khartoum state. RT-PCR was used and successfully amplified 551 bp within the M segment of the tripartite negative-sense single stranded RNA genome of RVFV. The virus was detected in female, male and larval stages of Culex and Anopheles species. The most affected human age interval was 15-29 years old followed by ≥ 45 years old, 30-44 years old, and then 5-14 years old. Regarding to the profession, housewives followed by farmers, students, shepherd, workers and the free were more vulnerable to the infection. Furthermore, connection between

  11. Presence of Viral RNA and Proteins in Exosomes from Cellular Clones Resistant to Rift Valley Fever Virus Infection.

    Science.gov (United States)

    Ahsan, Noor A; Sampey, Gavin C; Lepene, Ben; Akpamagbo, Yao; Barclay, Robert A; Iordanskiy, Sergey; Hakami, Ramin M; Kashanchi, Fatah

    2016-01-01

    Rift Valley Fever Virus (RVFV) is a RNA virus that belongs to the genus Phlebovirus, family Bunyaviridae. It infects humans and livestock and causes Rift Valley fever. RVFV is considered an agricultural pathogen by the USDA, as it can cause up to 100% abortion in cattle and extensive death of newborns. In addition, it is designated as Category A pathogen by the CDC and the NIAID. In some human cases of RVFV infection, the virus causes fever, ocular damage, liver damage, hemorrhagic fever, and death. There are currently limited options for vaccine candidates, which include the MP-12 and clone 13 versions of RVFV. Viral infections often deregulate multiple cellular pathways that contribute to replication and host pathology. We have previously shown that latent human immunodeficiency virus-1 (HIV-1) and human T-cell lymphotropic virus-1 (HTLV-1) infected cells secrete exosomes that contain short viral RNAs, limited number of genomic RNAs, and viral proteins. These exosomes largely target neighboring cells and activate the NF-κB pathway, leading to cell proliferation, and overall better viral replication. In this manuscript, we studied the effects of exosome formation from RVFV infected cells and their function on recipient cells. We initially infected cells, isolated resistant clones, and further purified using dilution cloning. We then characterized these cells as resistant to new RVFV infection, but sensitive to other viral infections, including Venezuelan Equine Encephalitis Virus (VEEV). These clones contained normal markers (i.e., CD63) for exosomes and were able to activate the TLR pathway in recipient reporter cells. Interestingly, the exosome rich preparations, much like their host cell, contained viral RNA (L, M, and S genome). The RNAs were detected using qRT-PCR in both parental and exosomal preparations as well as in CD63 immunoprecipitates. Viral proteins such as N and a modified form of NSs were present in some of these exosomes. Finally, treatment of

  12. Experiencias en la vigilancia epidemiológica de agentes patógenos transmitidos por alimentos a través de electroforesis en campo pulsado (PFGE en el Perú Experiences in the epidemiological surveillance of foodborne pathogens by pulsed field gel electrophoresis (PFGE in Peru

    Directory of Open Access Journals (Sweden)

    María Luz Zamudio

    2011-03-01

    Full Text Available Las enfermedades transmitidas por alimentos (ETA y otras enfermedades entéricas infecciosas ocurren a menudo como brotes y son causa de morbilidad y mortalidad en todo el mundo. En el Perú, son un importante problema de salud pública y son causados por una gran variedad de agentes infecciosos. Para la investigación epidemiológica se utiliza una variedad de métodos de tipificación. Una de las herramientas más importantes en la subtipificación molecular de patógenos bacterianos es la técnica de la electroforesis en campo pulsado (PFGE, que es un método altamente resolutivo que permite la discriminación entre diferentes aislamientos bacterianos epidemiológicamente relacionados. El Instituto Nacional de Salud (INS del Perú integra las redes WHO Global Foodborne Infections Network y la Red PulseNet América Latina y Caribe, con quienes comparte los perfiles genéticos de las cepas patógenas aisladas, permitiendo comparar los genotipos de cepas semejantes halladas en diferentes países y reconocer la ocurrencia de brotes epidémicos en la región, fortaleciendo el sistema de vigilancia epidemiológica regional y generando una rápida respuesta conjunta entre países. Se presenta la experiencia de los dos últimos años sobre los avances en la utilización de estas herramientas estratégicas que nos ha permitido caracterizar patrones de genotipo de principales patógenos implicados en ETA a partir de aislamientos recuperados de la red de laboratorios del Perú.Foodborne diseases and other enteric infections often occur as outbreaks and cause morbidity and mortality all over the world. In Perú, they represent a serious public health problem, and are caused by a great variety of infectious agents. For epidemiological research, a wide array of typification methods are used. One of the most important tools for the molecular subtyping of bacterial pathogens is the Pulsed Field Gel Electrophoresis (PFGE, which is a highly precise method that allows the discrimination between different bacterial isolates which are epidemiologically related. The Instituto Nacional de Salud del Perú (INS is part of the WHO Global Foodborne Infections Network (WHO-GFN and of the PulseNet Latin American and Caribbean Net (PN-AL & C, with whom it shares the genetic profiles of the isolated pathogenic strains, so that it is possible to compare de genotypes of similar strains found in different countries and to identify the occurrence of epidemic outbreaks in the region, strengthening the regional system of epidemiological surveillance and generating a rapid, coordinated response between the countries. We present the two last years´ experience including the advances in the use of these strategic tools that have allowed us to characterize genotype patterns implicated in foodborne diseases from isolates recovered in the laboratory network of Peru.

  13. Serosurvey of tick-borne pathogens in dogs from urban and rural areas from Parana State, Brazil Avaliação sorológica de patógenos transmitidos por carrapatos em cães urbanos e rurais do estado do Paraná, Brasil

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    Thállitha Samih Wischral Jayme Vieira

    2013-03-01

    Full Text Available Considering the zoonotic potential of tick-borne disease (TBD agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of TBD agents and risk factors for exposure in two different canine populations from Parana State, Southern Brazil. A total of 138 dog serum samples from urban (UA (n=68 and rural (RA (n=70 areas were tested with commercial ELISA rapid test for Anaplasma phagocytophilum, Ehrlichia canis and Borrelia burgdorferi antibodies and indirect immunofluorescence assay (IFAT for Babesia vogeli. An overall of 92∕138 (66.7% dogs, being 62∕68 (91.2% from UA and 30∕70 (42.9% from RA, were seropositive for at least one TBD agent. From the total number of dogs, sixty-two were positive for E. canis (44.9%, 19 (13.8% for A. phagocytophilum, and 64 (46.4% for B. vogeli. Anti-B. burgdorferi antibodies were not detected. Dogs from UA showed a higher percentage of tick infestation (p = 0.0135 and were highly associated with seropositivity to E. canis (p = 0.000005, A. phagocytophilum (p = 0.0001, and B. vogeli (p = 0.0012. In summary, the findings indicate that dogs from urban areas present higher potential risk exposure to TBD pathogens than those from rural areas.Considerando o potencial zoonótico das doenças transmitidas por carrapatos (DTCs e que os cães podem atuar como sentinelas para infecções em humanos, os objetivos deste estudo foram determinar a soroprevalência de agentes das DTCs e fatores de risco para a exposição em duas diferentes populações caninas do Estado do Paraná, região Sul do Brasil. Um total de 138 amostras de soro de cães de área urbana (AU (n = 68 e rural (AR (n = 70 foram testadas utilizando um teste de ELISA comercial rápido para detecção de anticorpos contra Anaplasma phagocytophilum, Ehrlichia canis e Borrelia burgdorferi e imunofluorescência indireta (IFI para Babesia vogeli. Um total de 92∕138 (66,7% cães, sendo 62∕68 (91,2% da AU e 30∕70 (42,9% da AR, foram soropositivos para pelo menos um agente. Do número total de amostras, sessenta e duas (44,9% foram positivas para E. canis, 19 (13,8% para A. phagocytophilum e 64 (46,4% para B. canis vogeli. Anticorpos anti-B. burgdorferi não foram detectados. Os cães da AU apresentaram o maior percentual de infestação por carrapatos (p = 0,0135 e foram altamente associados com a positividade para E. canis (p = 0,000005, A. phagocytophilum (p = 0,0001 e B. vogeli (p = 0,0012. Em resumo, nossos achados indicam que cães de áreas urbanas têm um maior risco potencial de exposição a agentes patogênicos das DTCs comparados aos das áreas rurais.

  14. Mejoramiento de la latencia de la red mediante el cambio de tamaño de búfer para aplicaciones FTP utilizando el modelo cliente/servidor según el tamaño promedio de los archivos a ser transmitidos

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    Juan de Dios Murillo

    2016-03-01

    al modelo cliente/servidor.En esta investigación se analizaran ciertas características del modelo cliente/Servidor que lo convierten en una excelente opción para generar ganancias en empresas, tanto en tiempo, costos, seguridad y desempeño de todo el sistema y cómo se puede mejorar para obtener un mejor uso de ancho de banda.

  15. Oropuche virus: A virus present but ignored

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    Salim Mattar V.

    2015-09-01

    Full Text Available Bunyaviruses are RNA viruses that affect animals and plants; they have five genera and four of them affect humans: Orthobunyavirus, Nairovirus, Phlebovirus and Hantavirus. All of them are Arbovirus, except Hantavirus. The Orthobunyaviruses comprise Oropouche, Tahyna, La Crosse virus, California encephalitis virus and Heartland virus recently discovered (1. Except for Heartland virus which is transmitted by ticks of the genus Amblyoma, these Phleboviruses have as vectors mosquitoes, which bite small mammals which are able to be as reservoirs amplifiers.

  16. Epidemiology of Rift Valley fever and risks for its introduction into Europe

    International Nuclear Information System (INIS)

    Full text: Rift Valley fever virus (RVFV), a member of the Phlebovirus genus of the Bunyaviridae family, is a causative agent of Rift Valley fever (RVF), a mosquito-borne viral zoonotic disease that poses a significant health threat to domestic ruminants and humans in Africa. Infection with RVFV in livestock is characterized by an acute hepatitis, abortion and high mortality rates in new borne animals. Humans infected with RVFV typically develop a mild self-limited febrile illness, but retinal degeneration, severe encephalitis, fatal hepatitis and hemorrhagic manifestations occur in small proportion of patients. Since original isolation of the virus in 1930 following an outbreak of 'enzootic hepatitis' on a sheep farm near Naivasha in the Rift Valley region of Kenya, for the next four decades, epizootics were recorded only in eastern and southern Africa. However, in 1977 RVF spread to northern Africa, in 1987 to West Africa, and in 2000 to the Arabian Peninsula. The latter spread represents the first outbreaks of RVF in livestock and humans recognised outside Africa. The fate of the virus during inter epizootic periods has long constituted a central enigma in the epidemiology of the disease. Cryptic maintenance and transmission cycles have been hypothesized but the exact mechanisms are not well understood. The current prevailing hypothesis is that at least in eastern and southern Africa, RVFV is maintained in the eggs of floodwater breeding aedine mosquitoes, which breed in isolated grassland depressions called dambos. The aedine mosquitoes overwinter as eggs, which can survive for long periods in dried mud. Flooding of the dambos during heavy and prolong rainfalls results in hatching of transovarially infected aedine mosquitoes followed by virus transmission to livestock. Infected livestock serve as a source of infection for culicines and anopheline mosquitoes, which act as epizootic vectors. Biting flies such as stomoxids, phlebotomids, midges, and simulids might

  17. CLONING AND EXPRESSION OF THE CRIMEAN-CONGO HEMORRHAGIC FEVERVIRUS GLYCOPROTEINS

    OpenAIRE

    Sliwa, Mariam

    2009-01-01

    Crimean-Congo Hemorrhagic Fever (CCHF) is a worldwide tick-borne disease that originally belongs to the Bunyaviridae family, the genus Nairovirus. In addition to infection from ticks, humans become infected if any contact with infected blood or tissue material occurs. To study the disease, several methods such as real-time Polymerase Chain Reaction, enzyme-linked immunosorbent assay and Immunofluorescence assay are used for detection of the virus. All viruses in Bunyaviridae consists of three...

  18. Sandfly Fever Sicilian Virus, Algeria

    OpenAIRE

    Izri, Arezki; Temmam, Sarah; Moureau, Grégory; Hamrioui, Boussad; de Lamballerie, Xavier; Charrel, Rémi N.

    2008-01-01

    To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest SFSV is present in Algeria.

  19. Early Bunyavirus-Host Cell Interactions

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    Amelina Albornoz

    2016-05-01

    Full Text Available The Bunyaviridae is the largest family of RNA viruses, with over 350 members worldwide. Several of these viruses cause severe diseases in livestock and humans. With an increasing number and frequency of outbreaks, bunyaviruses represent a growing threat to public health and agricultural productivity globally. Yet, the receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely uncharacterized. The focus of this review is on the early steps of bunyavirus infection, from virus binding to penetration from endosomes. We address current knowledge and advances for members from each genus in the Bunyaviridae family regarding virus receptors, uptake, intracellular trafficking and fusion.

  20. Early Bunyavirus-Host Cell Interactions.

    Science.gov (United States)

    Albornoz, Amelina; Hoffmann, Anja B; Lozach, Pierre-Yves; Tischler, Nicole D

    2016-01-01

    The Bunyaviridae is the largest family of RNA viruses, with over 350 members worldwide. Several of these viruses cause severe diseases in livestock and humans. With an increasing number and frequency of outbreaks, bunyaviruses represent a growing threat to public health and agricultural productivity globally. Yet, the receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely uncharacterized. The focus of this review is on the early steps of bunyavirus infection, from virus binding to penetration from endosomes. We address current knowledge and advances for members from each genus in the Bunyaviridae family regarding virus receptors, uptake, intracellular trafficking and fusion. PMID:27213430

  1. The Andes Hantavirus NSs Protein Is Expressed from the Viral Small mRNA by a Leaky Scanning Mechanism

    OpenAIRE

    Vera-Otarola, Jorge; Solis, Loretto; Soto-Rifo, Ricardo; Ricci, Emiliano P.; Pino, Karla; Tischler, Nicole D.; Ohlmann, Théophile; Darlix, Jean-Luc; López-Lastra, Marcelo

    2012-01-01

    The small mRNA (SmRNA) of all Bunyaviridae encodes the nucleocapsid (N) protein. In 4 out of 5 genera in the Bunyaviridae, the smRNA encodes an additional nonstructural protein denominated NSs. In this study, we show that Andes hantavirus (ANDV) SmRNA encodes an NSs protein. Data show that the NSs protein is expressed in the context of an ANDV infection. Additionally, our results suggest that translation initiation from the NSs initiation codon is mediated by ribosomal subunits that have bypa...

  2. Haemaphysalis longicornis Ticks as Reservoir and Vector of Severe Fever with Thrombocytopenia Syndrome Virus in China

    OpenAIRE

    Luo, Li-Mei; Zhao, Li; Wen, Hong-Ling; Zhang, Zhen-Tang; Liu, Jian-Wei; Fang, Li-Zhu; Xue, Zai-Feng; Ma, Dong-Qiang; Zhang, Xiao-Shuang; Ding, Shu-Jun; Lei, Xiao-Ying; Yu, Xue-jie

    2015-01-01

    Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever in East Asia caused by SFTS virus (SFTSV), a newly discovered phlebovirus. The Haemaphysalis longicornis tick has been suspected to be the vector of SFTSV. To determine whether SFTSV can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of H. longicornis ticks and between ticks and mice. Using reverse transcription PCR, ...

  3. Use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the G2 glycoprotein of Rift Valley fever virus.

    OpenAIRE

    K. Keegan; Collett, M S

    1986-01-01

    Four distinct antigenic determinants along the G2 glycoprotein encoded by the M segment RNA of the Phlebovirus Rift Valley fever virus were localized. These epitopes were defined by four monoclonal antibodies, three of which were capable of neutralizing virus infectivity; one was nonneutralizing. Immunoprecipitation by these monoclonal antibodies of either denatured or native antigen characterized the epitopes as having linear or higher order structure. Molecular cloning of G2 glycoprotein-co...

  4. Molecular evolution of Puumala hantavirus in Fennoscandia: phylogenetic analysis of strains from two recolonization routes

    DEFF Research Database (Denmark)

    Asikainen, Kari; Hänninen, Tarja; Henttonen, Heikki; Niemimaa, Jukka; Laakkonen, Juha; Andersen, Hans Kerzel; Bille, Nils; Leirs, Herwig; Vaheri, Antti; Plyusnin, Alexander

    2000-01-01

    Like other members of the genus Hantavirus in the family Bunyaviridae, Puumala virus (PUUV) is thought to be co-evolving with its natural host, the bank vole Clethrionomys glareolus. To gain insight into the evolutionary history of PUUV in northern Europe during the last post-glacial period, we...

  5. Evidence for Culicoides obsoletus group as vector for Schmallenberg virus in Denmark

    DEFF Research Database (Denmark)

    Rasmussen, Lasse Dam; Kristensen, Birgit; Kirkeby, Carsten;

    Bunyaviridae family and is closely related to Shamonda and Akabane viruses. These viruses are transmitted by insect vectors (including biting midges (Culicoides sp.) and mosquitoes). To determine whether these insects may act as vectors for SBV, biting midges (Culicoides spp.) caught in October 2011, in the...

  6. Geographical distribution and surveillance of Crimean-Congo hemorrhagic fever in Iran

    DEFF Research Database (Denmark)

    Chinikar, Sadegh; Ghiasi, Seyed Mojtaba; Moradi, Maryam;

    2010-01-01

    Crimean-Congo hemorrhagic fever (CCHF) is viral hemorrhagic fever caused by CCHF virus, which belongs to the family Bunyaviridae and the genus Nairovirus. The virus is transmitted to humans via contact with blood and tissue from infected livestock, a tick bite, or contact with an infected person...

  7. Severe Crimean-Congo haemorrhagic fever presented with massive retroperitoneal haemorrhage that recovered without antiviral treatment

    DEFF Research Database (Denmark)

    Gharabaghi, Mehrnaz Asadi; Chinikar, Sadegh; Ghiasi, Seyyed Mojtaba;

    2011-01-01

    Crimean-Congo haemorrhagic fever (CCHF) is a tickborne viral zoonosis with up to 50% mortality in humans caused by CCHF virus belonging to the genus Nairovirus, family Bunyaviridae. The geographical distribution of CCHF cases corresponds closely with the distribution of principle tick vectors that...

  8. Evidence for widespread infection of African bats with Crimean-Congo hemorrhagic fever-like viruses

    Czech Academy of Sciences Publication Activity Database

    Müller, M. A.; Devignot, S.; Lattwein, E.; Corman, V. M.; Maganga, G. D.; Gloza-Rausch, F.; Binger, T.; Vallo, Peter; Emmerich, P.; Cottontail, V. M.; Tschapka, M.; Oppong, S.; Drexler, J. F.; Weber, F.; Leroy, E. M.; Drosten, C.

    2016-01-01

    Roč. 6, č. 26637 (2016), s. 26637. ISSN 2045-2322 Institutional support: RVO:68081766 Keywords : sheep disease virus * family Bunyaviridae * serological relationships * antibody-response * migratory birds * rapid detection * viral load * ticks * nairovirus * genus Subject RIV: FN - Epidemiology, Contagious Diseases ; Clinical Immunology Impact factor: 5.578, year: 2014

  9. Brus Laguna virus, a Gamboa bunyavirus from Aedeomyia squamipennis collected in Honduras.

    Science.gov (United States)

    Calisher, C H; Lazuick, J S; Sudia, W D

    1988-10-01

    A virus isolate from Aedeomyia squamipennis collected in Honduras in 1967 was identified as a member of the Gamboa serogroup (family Bunyaviridae, genus Bunyavirus). This is the ninth Gamboa serogroup virus and the eighth shown to be a distinct serotype. PMID:2903690

  10. Occurrence of Tomato spotted wilt virus in Stevia rebaudiana and Solanum tuberosum in Northern Greece

    NARCIS (Netherlands)

    Chatzivassiliou, E.K.; Peters, D.; Lolas, P.

    2007-01-01

    Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae) was first reported in Greece during 1972 (3) and currently is widespread in the central and northern part of the country infecting several cultivated and wild plant species (1,2). In June 2006, virus-like symptoms similar to th

  11. Dynamics of genetic diversity of Tomato spotted wilt virus in the United States

    Science.gov (United States)

    Among known tospoviruses, Tomato spotted wilt virus (TSWV; genus Tospovirus, family Bunyaviridae) continues to be the major viral disease affecting a wide range of agricultural and horticultural crops worldwide. Like many other RNA viruses, TSWV is known to maintain heterogeneous and divergent popul...

  12. Towards understanding TSWW particle assembly: analysis of the intracellular behavior of the viral structural proteins

    NARCIS (Netherlands)

    Ribeiro, D.M.O.G.

    2007-01-01

    At the onset of the studies presented in this thesis, it was already known that the assembly of the enveloped particle of Tomato spotted wilt virus (TSWV; family Bunyaviridae) in the infected plant cell was featured by a number of interesting phenomena. This process involves enwrapment of the viral

  13. Transmissão por mãos contaminadas e ausência de transmissão embrionária do vírus do amarelo letal do mamoeiro Transmission by contaminated hands and absence of embryonary seed transmission of papaya lethal yellowing virus

    OpenAIRE

    Armando C. M. Saraiva; Waldelice O. de Paiva; Francisco A.C. Rabelo Filho; J. Albérsio A. Lima

    2006-01-01

    O vírus do amarelo letal do mamoeiro ("Papaya lethal yellowing virus", PLYV) é responsável por uma das principais doenças do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido por solo, água, instrumentos de corte contaminados e por inoculação mecânica em condições experimentais. Na presente pesquisa, avaliou-se a transmissão do PLYV por mãos contaminadas e sua presença em embriões de sementes de frutos infetados. O PLYV foi transmitido por mãos contaminadas, e mãos ...

  14. Revista de revistas

    OpenAIRE

    Revista, Facultad de Medicina

    2011-01-01

    British Medical lnformation Service. 3, Hanover Street.-London, w. 1. Algunas consideraciones prácticas acerca del control del tifus transmitido por piojos en Gran Bretaña a la luz de la experiencia de Rusia, Polonia. Rumania y China. Nueva nota sobre un método para teñir parásitos palúdicos en frottis gruesos de sangre Shock en el recién nacido

  15. Genetic Diversity of Crimean Congo Hemorrhagic Fever Virus Strains from Iran

    OpenAIRE

    Sadegh Chinikar; Saeid Bouzari; Mohammad Ali Shokrgozar; Ehsan Mostafavi; Tahmineh Jalali; Sahar Khakifirouz; Norbert Nowotny; Fooks, Anthony R.; Nariman Shah-Hosseini

    2016-01-01

    Background: Crimean Congo hemorrhagic fever virus (CCHFV) is a member of the Bunyaviridae family and Nairovirus genus. It has a negative-sense, single stranded RNA genome approximately 19.2 kb, containing the Small, Medium, and Large segments. CCHFVs are relatively divergent in their genome sequence and grouped in seven distinct clades based on S-segment sequence analysis and six clades based on M-segment sequences. Our aim was to obtain new insights into the molecular epidemiology of CCHFV i...

  16. Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran

    OpenAIRE

    Chinikar, Sadegh; Shah-Hosseini, Nariman; Bouzari, Saeid; Shokrgozar, Mohammad Ali; MOSTAFAVI, Ehsan; Jalali, Tahmineh; Khakifirouz, Sahar; Groschup, Martin H; Niedrig, Matthias

    2016-01-01

    Background: Crimean-Congo Hemorrhagic Fever Virus (CCHFV) belongs to genus Nairovirus and family Bunyaviridae. The main aim of this study was to investigate the extent of recombination in S-segment genome of CCHFV in Iran. Methods: Samples were isolated from Iranian patients and those available in GenBank, and analyzed by phyloge­netic and bootscan methods. Results: Through comparison of the phylogenetic trees based on full length sequences and partial fragments in the S-segment genome ...

  17. First International External Quality Assessment of Molecular Detection of Crimean-Congo Hemorrhagic Fever Virus

    OpenAIRE

    Escadafal, Camille; Ölschläger, Stephan; Avšič-Županc, Tatjana; Papa, Anna; Vanhomwegen, Jessica; Wölfel, Roman; Mirazimi, Ali; Teichmann, Anette; Donoso Mantke, Oliver; Niedrig, Matthias

    2012-01-01

    Crimean-Congo hemorrhagic fever (CCHF) is a zoonosis caused by a Nairovirus of the family Bunyaviridae. Infection is transmitted to humans mostly by Hyalomma ticks and also by direct contact with the blood or tissues of infected humans or viremic livestock. Clinical features usually include a rapid progression characterized by hemorrhage, myalgia and fever, with a lethality rate up to 30%. CCHF is one of the most widely distributed viral hemorrhagic fevers and has been reported in Africa, the...

  18. Evolutionary and phenotypic analysis of live virus isolates suggests arthropod origin of a pathogenic RNA virus family

    OpenAIRE

    Marklewitz, Marco; Zirkel, Florian; Kurth, Andreas; Drosten, Christian; Junglen, Sandra

    2015-01-01

    Knowledge of the origin and evolution of viruses provides important insight into virus emergence involving the acquisition of genes necessary for the infection of new host species or the development of pathogenicity. The family Bunyaviridae contains important arthropod-borne pathogens of humans, animals, and plants. In this study, we provide a comprehensive characterization of two novel lineages of insect-specific bunyaviruses that are in basal phylogenetic relationship to the rodent-borne ha...

  19. CRIMEAN CONGO HEMORRHAGIC FEVER - AN ARTICLE REVIEW

    OpenAIRE

    Khan Najam Ali; Jaiswal Anushree; Choudhray Reenu; Abid Mohammad; Kishore Kamal

    2011-01-01

    Crimean–Congo hemorrhagic fever (CCHF) caused by negative-sense, single-stranded RNA virus in the genus Nairovirus, family Bunyaviridae. (CCHF) is a tick-borne infectious disease characterized by fever, malaise, headache, nausea, vomiting, diarrhoea, sore throat, muscle aches, hemorrhage and thrombocytopenia. CCHF has the most extensive geographic range of the medically significant tick-borne viruses occurring in Africa, Europe and Asia & found recently in India (Ahmadabad) in 2011,become a s...

  20. Phylogenetic analysis of a newfound bat-borne hantavirus supports a laurasiatherian host association for ancestral mammalian hantaviruses

    OpenAIRE

    Witkowski, P. T.; Drexler, J. F.; Kallies, R.; Lickova, M; Bokorova, S.; Mananga, G. D.; Szemes, T.; Leroy, Eric; Kruger, D H; Drosten, C.; Klempa, B.

    2016-01-01

    Until recently, hantaviruses (family Bunyaviridae) were believed to originate from rodent reservoirs. However, genetically distinct hantaviruses were lately found in shrews and moles, as well as in bats from Africa and Asia. Bats (order Chiroptera) are considered important reservoir hosts for emerging human pathogens. Here, we report on the identification of a novel hantavirus, provisionally named Makokou virus (MAKV), in Noack's Roundleaf Bat (Hipposideros ruber) in Gabon, Central Africa. Ph...

  1. Hantaviruses: Rediscovery and New Beginnings

    OpenAIRE

    Yanagihara, Richard; Gu, Se Hun; Arai, Satoru; Kang, Hae Ji; Song, Jin-Won

    2014-01-01

    Virus and host gene phylogenies, indicating that antigenically distinct hantaviruses (family Bunyaviridae, genus Hantavirus) segregate into clades, which parallel the molecular evolution of rodents belonging to the Murinae, Arvicolinae, Neotominae and Sigmodontinae subfamilies, suggested co-divergence of hantaviruses and their rodent reservoirs. Lately, this concept has been vigorously contested in favor of preferential host switching and local host-specific adaptation. To gain insights into ...

  2. Gene S characterization of Hantavirus species Seoul virus isolated from Rattus norvegicuson an Indonesian island

    OpenAIRE

    Dian Perwitasari; Ima Nurisa Ibrahim; Andi Yasmon

    2014-01-01

    AbstrakLatar belakang: Hantavirus hidup dan berkembang biak di tubuh hewan pengerat, salah satunya Rattus norvegicus yang banyak ditemukan di daerah kepulauan di Indonesia. Hantavirus spesies Seoul virus (SEOV) adalah virus RNA negatif rantai tunggal yang termasuk dalam keluarga Bunyaviridae, mempunyai beberapa gen spesifik terutama gen S yang dapat dikembangkan untuk uji diagnostik. Tujuan penelitian ini ialah untuk mengetahui karakter dari gen S dari Hantavirus spesies Seoulvirus.Metode:Pad...

  3. The fundamental role of endothelial cells in hantavirus pathogenesis

    OpenAIRE

    Hepojoki, Jussi; Vaheri, Antti; Strandin, Tomas

    2014-01-01

    Hantavirus, a genus of rodent- and insectivore-borne viruses in the family Bunyaviridae, is a group of emerging zoonotic pathogens. Hantaviruses cause hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome in man, often with severe consequences. Vascular leakage is evident in severe hantavirus infections, and increased permeability contributes to the pathogenesis. This review summarizes the current knowledge on hantavirus interactions with hematopoietic and endothelial ...

  4. Electron Cryotomography of Tula Hantavirus Suggests a Unique Assembly Paradigm for Enveloped Viruses▿

    OpenAIRE

    Huiskonen, Juha T.; Hepojoki, Jussi; Laurinmäki, Pasi; Vaheri, Antti; Lankinen, Hilkka; Butcher, Sarah J.; Grünewald, Kay

    2010-01-01

    Hantaviruses (family Bunyaviridae) are rodent-borne emerging viruses that cause a serious, worldwide threat to human health. Hantavirus diseases include hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. Virions are enveloped and contain a tripartite single-stranded negative-sense RNA genome. Two types of glycoproteins, GN and GC, are embedded in the viral membrane and form protrusions, or “spikes.” The membrane encloses a ribonucleoprotein core, which consists of ...

  5. Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil

    OpenAIRE

    João Bosco Lima Gimaque; Michele de Souza Bastos; Wornei Silva Miranda Braga; Cintia Mara Costa de Oliveira; Márcia da Costa Castilho; Regina Maria Pinto de Figueiredo; Elizabeth dos Santos Galusso; Evaulino Ferreira Itapirema; Luiz Tadeu Moraes Figueiredo; Maria Paula Gomes Mourão

    2012-01-01

    Hantavirus disease is caused by the hantavirus, which is an RNA virus belonging to the family Bunyaviridae. Hantavirus disease is an anthropozoonotic infection transmitted through the inhalation of aerosols from the excreta of hantavirus-infected rodents. In the county of Itacoatiara in the state of Amazonas (AM), Brazil, the first human cases of hantavirus pulmonary and cardiovascular syndrome were described in July 2004. These first cases were followed by two fatal cases, one in the municip...

  6. Antibody levels to hantavirus in inhabitants of western Santa Catarina State, Brazil

    OpenAIRE

    William Marciel de Souza; Alex Martins Machado; Geonildo Rodrigo Disner; Everton Boff; Aline Rafaela da Silva Rodrigues Machado; Michelly de Padua; Luiz Tadeu Moraes Figueiredo; Gustavo Borba de Miranda

    2012-01-01

    Hantavirus cardiopulmonary syndrome (HCPS) is an infectious disease caused by hantaviruses of the family Bunyaviridae, and is transmitted by aerosols of excreta of infected rodents. The aim of the present study was to determine antibody levels to hantavirus in the population that lives at frontier of Brazil and Argentina. Participated of the study 405 individuals living in the municipalities of Bandeirante, Santa Helena, Princesa and Tunapolis, state of Santa Catarina, Brazil. IgG antibodies ...

  7. Cytokine expression during early and late phase of acute Puumala hantavirus infection

    OpenAIRE

    Sadeghi Mahmoud; Eckerle Isabella; Daniel Volker; Burkhardt Ulrich; Opelz Gerhard; Schnitzler Paul

    2011-01-01

    Abstract Background Hantaviruses of the family Bunyaviridae are emerging zoonotic pathogens which cause hemorrhagic fever with renal syndrome (HFRS) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. An immune-mediated pathogenesis is discussed for both syndromes. The aim of our study was to investigate cytokine expression during the course of acute Puumala hantavirus infection. Results We retrospectively studied 64 patients hospitalised with acute Puumala hantavirus i...

  8. Hantaviral Proteins: Structure, Functions, and Role in Hantavirus Infection

    OpenAIRE

    Muyangwa, Musalwa; Martynova, Ekaterina V.; Khaiboullina, Svetlana F.; Morzunov, Sergey P.; Rizvanov, Albert A.

    2015-01-01

    Hantaviruses are the members of the family Bunyaviridae that are naturally maintained in the populations of small mammals, mostly rodents. Most of these viruses can easily infect humans through contact with aerosols or dust generated by contaminated animal waste products. Depending on the particular Hantavirus involved, human infection could result in either hemorrhagic fever with renal syndrome or in Hantavirus cardiopulmonary syndrome. In the past few years, clinical cases of the Hantavirus...

  9. Hantavirus N Protein Exhibits Genus-Specific Recognition of the Viral RNA Panhandle▿

    OpenAIRE

    Mir, M A; Brown, B.; Hjelle, B; Duran, W. A.; Panganiban, A T

    2006-01-01

    A key genomic characteristic that helps define Hantavirus as a genus of the family Bunyaviridae is the presence of distinctive terminal complementary nucleotides that promote the folding of the viral genomic segments into “panhandle” hairpin structures. The hantavirus nucleocapsid protein (N protein), which is encoded by the smallest of the three negative-sense genomic RNA segments, undergoes in vivo and in vitro trimerization. Trimeric hantavirus N protein specifically recognizes the panhand...

  10. Insights into bunyavirus architecture from electron cryotomography of Uukuniemi virus

    OpenAIRE

    Överby, A. K.; Pettersson, R F; Grünewald, K; Huiskonen, J.T.

    2008-01-01

    Bunyaviridae is a large family of viruses that have gained attention as “emerging viruses” because many members cause serious disease in humans, with an increasing number of outbreaks. These negative-strand RNA viruses possess a membrane envelope covered by glycoproteins. The virions are pleiomorphic and thus have not been amenable to structural characterization using common techniques that involve averaging of electron microscopic images. Here, we determined the three-dimensional structure o...

  11. Serological Screening Suggests Presence of Schmallenberg Virus in Cattle, Sheep and Goat in the Zambezia Province, Mozambique

    OpenAIRE

    Blomström, A-L; Stenberg, H; Scharin, I; Figueiredo, J; Nhambirre, O; Abilio, A P; Fafetine, J; Berg, M.

    2014-01-01

    Schmallenberg virus (SBV) is a novel Orthobunyavirus within the family Bunyaviridae belonging to the Simbu serogroup. Schmallenberg virus infects ruminants and has since its discovery in the autumn 2011 been detected/spread to large parts of Europe. Most bunyaviruses are arboviruses, and SBV has been detected in biting midges in different European countries, suggesting that they may play a role in the transmission of the virus. It is not known how SBV was introduced to Europe and if SBV is pr...

  12. Sanidade, transmissão via semente e patogenicidade de fungos em sementes de Cedrela fissilis procedentes da região sul do Brasil

    Directory of Open Access Journals (Sweden)

    Marília Lazarotto

    2012-09-01

    Full Text Available http://dx.doi.org/10.5902/198050986617Pesquisas relacionadas à patologia de sementes de espécies florestais nativas, especialmente aquelas mais aprofundadas, são escassas. Assim, o objetivo deste estudo foi avaliar a sanidade das sementes de cedro através de dois métodos de detecção, avaliar a transmissão de fungos associados às sementes para as plântulas e avaliar a patogenicidade de fungos associados às sementes e transmitidos para as plântulas. Para tanto, sementes de seis procedências, dentre os estados do Rio Grande do Sul, Santa Catarina e Paraná, foram utilizadas. Estas foram submetidas a testes de sanidade, pelos métodos do papel-filtro e batata-dextrose-ágar (BDA, a teste de transmissão via sementes para as plântulas e a teste de patogenicidade de isolados de Rhizoctonia sp., patógeno escolhido por ser o mais frequente nos testes de sanidade e transmitido via semente. Os fungos encontrados em ambos os testes foram: Pestalotia sp., Rhizoctonia sp., Penicillium sp., Phomopsis sp., Rhizopus sp., Aspergillus sp. e Fusarium sp. Ambos os métodos podem ser utilizados para teste de sanidade em sementes de cedro. Os fungos transmitidos via semente para plântula foram Fusarium sp. e Rhizoctonia sp., sendo que, estes causaram danos nas raízes e posterior tombamento. Foi identificado um isolado de Rhizoctonia sp. patogênico para plântulas de cedro, causando os mesmos sintomas relatados no teste de transmissão.

  13. La identidad de género : masculino versus femenino

    OpenAIRE

    Gallegos Argüello, María del Carmen

    2012-01-01

    El presente trabajo tiene como finalidad analizar las características que definen el concepto de identidad y los elementos constitutivos de la identidad femenina y masculina que se han transmitido culturalmente a través de los procesos de socialización. Los sujetos construyen su actuar a partir de las referencias culturales que van aprendiendo a través de su proceso formativo. La diferencia sexual se da por la transmisión de ideas y valores distintos para cada individuo, por su...

  14. A relação de intermedicalidade nos Índios Truká, em Cabrobó - Pernambuco

    OpenAIRE

    Hítalo Thiago Gomes Vieira; Jacqueline Eyleen de Lima Oliveira; Rita de Cássia Maria Neves

    2013-01-01

    INTRODUÇÃO: Os conhecimentos tradicionais indígenas de saúde fundamentam-se em uma abordagem holística, cujo princípio é a harmonia de indivíduos, famílias e comunidades com o universo que os rodeia. Um dos desafios que antecede a atuação dos profissionais de saúde é o respeito à diferença, em que os conhecimentos e tecnologias da Biomedicina não devem ser transmitidos verticalmente, tornando-se imprescindível o reconhecimento da diversidade social e cultural dos povos indígenas. OBJETIVO: Id...

  15. Sorologia e sobrevivência do vírus do amarelo letal do mamoeiro Serology and surviving characteristics of Papaya lethal yellowing virus

    OpenAIRE

    Ana Lúcia Lopes do Nascimento; José Albersio de Araujo Lima; Aline Kelly Queiroz do Nascimento; Maria de Fátima Barros Gonçalves

    2010-01-01

    O vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV) é responsável por uma das principais doenças do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido através do solo, água, instrumento de corte e mãos contaminadas. A presente pesquisa teve como objetivo estudar as características biológicas, sorológicas e físicas de um isolado do vírus e avaliar sua sobrevivência em tecido seco infetado. O PLYV foi detectado por "Enzyme linked immunosorbent as...

  16. Antropología, comunicación y turismo: La mediación cultural en la construcción del espacio turístico de una comunidad de pescadores en Laguna, SC, Brasil Anthropology, Communication and Tourism: Cultural Mediation in Developing an Environment for Tourism in an Artisan Fishing Community in Laguna, SC

    OpenAIRE

    Rafael José dos Santos

    2005-01-01

    Basado en la etnografía de una comunidad de pescadores artesanales ubicada en Laguna, SC - Brasil, este trabajo analiza al destino turístico como un espacio culturalmente construido, es decir, un producto de prácticas a la vez sociales y simbólicas realizadas tanto por visitantes como residentes y mediadas por representaciones del imaginario moderno entre las que se encuentran aquellas que constituyen la matriz de los géneros de ficción transmitidos por los medios masivos de comunicación. Med...

  17. Manejo de plántulas de mango (Mangifera indica L.) Obtenidas in vitro para estudios de sanidad vegetal

    OpenAIRE

    Martha Elena López-Estrada; María Concepción Acosta-Rodríguez; Gabriel Otero-Colina; Alejandro Casimiro Michel-Aceves; David Heriberto Noriega-Cantú

    2008-01-01

    A partir de embriones inmaduros de mango se obtuvieron plántulas in vitro libres de contaminantes externos y transmitidos por semilla; luego se trasplantaron a suelo usando sólo materiales esterilizados. Se sembraron embriones de mango de 45 a 60 días de edad en medio B5 modificado; cuando las plántulas alcanzaron 10 cm de altura se trasplantaron a suelo + perlita expandida en recipientes de plástico especialmente diseñados y ahí se regaron con solución nutritiva universal. En la etapa in vi...

  18. Resistencia a insecticidas en Bemisia tabaci (Gennadius): nivel de resistencia, resistencias cruzadas y mecanismosimplicados

    OpenAIRE

    Fernández García, María Esther

    2013-01-01

    [SPA] La mosca blanca del tabaco, Bemisia tabaci (Gennadius, 1889), desde la década de los 90 es una de las plagas más importantes de muchos cultivos hortícolas y ornamentales españoles, tanto por sus daños directos como por la aparición de virus transmitidos por este insecto. A lo largo de estos años el uso excesivo de insecticidas para su control ha hecho que esta plaga desarrolle resistencias a diversos grupos de insecticidas. Para evitar esto y poder plantear estrategias de control adecua...

  19. Evolution and molecular characterization of tick-borne Anaplasmataceae and implications for pathogen diagnostics and control.

    OpenAIRE

    Cabezas Cruz, Alejandro

    2014-01-01

    Esta tesis se concentra en la caracterización molecular de patógenos transmitidos por garrapatas, los cuales han cobrado una gran importancia en los últimos años debido a su impacto en la economía agropecuaria, y en la salub pública de países tanto desarrollados como en vía de desarrollo (Perez y col., 2006; Rikihisa, 2010; Aubry y Geale, 2011). Particularmente la tesis usa una combinación de herramientas moleculares, bioinformaticas y filogenéticas para estudiar la variación genética de dos...

  20. Colostro humano: fonte natural de probióticos? Human colostrum: a natural source of probiotics?

    OpenAIRE

    Franz R. Novak; João Aprígio Guerra de Almeida; Vieira, Graciete O; Borba Luciana M.

    2001-01-01

    OBJETIVOS: o presente trabalho foi desenvolvido com o objetivo de obter dados sobre a microbiota do colostro humano, visando correlacioná-la com a possibilidade de que seja uma fonte natural de probióticos, que seriam transmitidos da mãe para o filho durante a amamentação natural. MÉTODOS: foram estudados, em 70 amostras de colostro humano ordenhado, os seguintes microorganismos: mesófilos, termodúricos, psicrotróficos, proteolíticos, proteolíticos-psicrotróficos, lipolíticos, bolores e leved...

  1. Dibujo y pensamiento en la obra de Rafael Masó

    OpenAIRE

    Marañón González, Rafael Carlos

    2002-01-01

    Rafael Masó, arquitecto noucentista, es además, el artista adecuado para el análisis de una obra totalmente gráfica.Dibujos, escritos, gráficos y bocetos, son los elementos idóneos para que las ideas sobre la expresión gráfica, queden reflejados en esta Tesis Doctoral.Para conocer su obra gráfica, es necesario llegar a lo más profundo de sus pensamientos transmitidos por historiadores y la Familia Masó. Tanto su obra arquitectónica, como sus dibujos, son conocidos por expertos y estudiosos ...

  2. Técnicas de monitorización del patrimonio construido : desarrollo y aplicación

    OpenAIRE

    Pérez Herreros, Daniel Gonzalo

    2014-01-01

    RESUMEN: Las estructuras presumen de su belleza a través del diseño y sufren de sus problemas como consecuencia de sus daños. Como evidencia de lo primero, las estructuras históricas constituyen una parte importante del patrimonio arquitectónico y cultural, reconocido como un frágil e insustituible recurso que debe ser conservado y transmitido a las generaciones futuras, en su máximo esplendor de materiales y formas. Y es que al mismo tiempo son la prueba evidente de la vida y la historia de ...

  3. Estrategia didáctica de aula para la enseñanza del tema carbohidratos en la panela, dirigida al programa de formación: Técnico en Agroindustria Panelera

    OpenAIRE

    Rodríguez Rojas, Camilo Andrés

    2015-01-01

    A partir de la naturaleza intuitiva del conocimiento empírico acumulado y transmitido en la elaboración artesanal de panela, sumado a recomendaciones técnicas para la elaboración y estabilización de miel de caña, se diseñó una cartilla para el aprendizaje situado de los carbohidratos en la panela, dirigido a estudiantes del programa de formación Técnico en Agroindustria Panelera. Se busca contribuir al proceso de capacitación de la población y a la vez propiciar innovación y mejor calidad en ...

  4. Efecto leishmanicida in vitro e in vivo de extractos derivados de Ocotea macrophylla y Zanthoxyllum monophyllum

    OpenAIRE

    Chávez Enciso, Norberto Andrey

    2013-01-01

    El parásito protozoario conocido como Leishmania Spp. es el causante de una enfermedad infecciosa definida como leishmaniosis. Este parásito es transmitido por vectores hematófagos del género Lutzomia y Phlebotomus (de alta incidencia en los países en desarrollo como Colombia); manifestándose en mayor proporción en las zonas rurales y en la población más vulnerable, lo cual dificulta en ocasiones, el acceso tanto al diagnóstico a tiempo y consecuentemente limita el inicio del tratamiento. Est...

  5. Caracterización molecular de virus emergentes patógenos de tomate y pimiento y virus persistentes de aguacate

    OpenAIRE

    Villanueva Montiel, Francisco

    2014-01-01

    La mayoría de virus de plantas han sido descubiertos por producir enfermedades en especies de interés agronómico. Estos virus están asociados al huésped en una relación a corto plazo: infectan la planta, se replican rápidamente causando síntomas, son transmitidos horizontalmente por contacto o mediante un vector biológico y finalmente se convierten en infecciones crónicas o provocan la muerte de su hospedador. Sin embargo, también existen virus que mantienen un estilo de vida persistente en l...

  6. Los manuales escritos por matronas europeas (1609-1710); el origen de cuidados humanizados en el embarazo

    OpenAIRE

    Martínez Rojo, Carmen; Siles González, José; Martínez Roche, María Emilia

    2014-01-01

    Objetivo principal: Analizar los cuidados administrados por matronas a mujeres en su embarazo en el siglo XVII transmitidos a través de los manuales escritos por las mismas parteras. Metodología: Metodología cualitativa, desde la hermenéutica y la historia cultural a través de técnicas etnográfico-narrativas mediante la lectura de cinco manuales (1609-1710), categorizando los textos siguiendo el modelo dialéctico de construcción-deconstrucción. Resultados principales: Obtuvimos como resultado...

  7. Charlie and the chocolate factory: adaptación y traducción

    Directory of Open Access Journals (Sweden)

    Ana María Castro Ortíz

    2008-05-01

    Full Text Available En este estudio se presenta la relación existente entre el discurso literario, el cinematográfico y el traductivo, por medio de un análisis descriptivo de algunos apartes de la obra literaria titulada "Charlie y la fábrica de chocolates", sus dos adaptaciones al cine y la subtitulación al español de la segunda adaptación. De esta manera, se evidencian algunas variaciones de las características propias de los personajes que pueden influir en el mensaje transmitido en la obra literaria original.

  8. Estudo de vulnerabilidades em aplicações web e o seu reflexo em domínios Portugueses

    OpenAIRE

    Monteiro, Nuno Miguel da Silva

    2015-01-01

    Muito se tem falado sobre revolução tecnológica e do aparecimento constante de novas aplicações Web, com novas funcionalidades que visam facilitar o trabalho dos utilizadores. Mas será que estas aplicações garantem que os dados transmitidos são tratados e enviados por canais seguros (protocolos)? Que garantias é que o utilizador tem que mesmo que a aplicação utilize um canal, que prevê a privacidade e integridade de dados, esta não apresente alguma vulnerabilidade pondo em caus...

  9. Comparação de três receptores GPS para uso em agricultura de precisão Comparison of three GPS receiver for precision agriculture uses

    OpenAIRE

    Marcelo C. C. Stabile; Luiz A. Balastreire

    2006-01-01

    Diversos equipamentos que se utilizam dos sinais transmitidos pelo Sistema de Posicionamento Global (GPS) têm sido empregados na Agricultura de Precisão. Neste estudo, foi feita uma comparação de três receptores comerciais no intuito de verificar suas acurácias. As principais qualidades medidas foram a repetibilidade dos dados e a estabilidade do sistema. O estudo foi conduzido em campo aberto, na área experimental da ESALQ/USP, com três linhas paralelas de 50 m espaçadas 10 m entre si. A col...

  10. “De frente pro crime”: cultura organizacional e socialização dos peritos ingressantes no departamento de criminalística do instituto-geral de perícias do Rio Grande do Sul

    OpenAIRE

    Neusa Rolita Cavedon

    2010-01-01

    A pesquisa objetivou verificar como determinados aspectos da cultura organizacional do Departamento de Criminalística são transmitidos aos neófitos, por meio do curso de formação de peritos; quem são esses ingressantes; qual a significação que o curso e as futuras atividades a serem desenvolvidas possuem para eles. Os dados foram obtidos mediante o fazer etnográfico, tendo por técnicas a observação participante, realizada com a presença da pesquisadora no módulo Local de Crime contra a Vida, ...

  11. A cultura organizacional: o impacto visível de uma dimensão invisível

    OpenAIRE

    Santos, Joana Vieira; Gonçalves, Gabriela

    2010-01-01

    No quotidiano, muitas vezes sem que se apercebam, os sujeitos são influenciados pelas características culturais do meio em que vivem. A cultura é considerada toda a totalidade dos padrões de comportamento socialmente transmitidos, artes, crenças e muitos outros processos considerados a expressão de um determinado período, classe, comunidade ou população (Choudhry, Fang e Mohamed, 2007). Ao nível organizacional encontramos um conjunto de valores, normas e padrões de comportamento que influenci...

  12. Development and implementation of a selective change-driven vision sensor for high speed movement analysis

    OpenAIRE

    Zuccarello, Pedro Diego

    2013-01-01

    Un sistema de vision artificial esta compuesto, en su forma más basica, por un sensor VLSI, habitualmente fabricado en tecnología CMOS o CCD, y una etapa de procesado. En la gran mayoría de los sistemas de visión artificial implementados hoy en día la etapa sensora del sistema consiste en un sensor de imágenes tradicional. Este tipo de sensores trabajan bajo unos principios muy simples y conocidos: el nivel de iluminación del entorno es muestreado y transmitido a intervalos de tiempo regulare...

  13. Número mais provável de Salmonella isoladas de carcaças de frango resfriadas Most probable number of Salmonella isolated from refrigerated broiler carcasses

    OpenAIRE

    Anderlise Borsoi; Hamilton Luis de Souza Moraes; Carlos Tadeu Pippi Salle; Vladimir Pinheiro do Nascimento

    2010-01-01

    A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analis...

  14. Sistema OXPHOS: integración funcional de dos genomas

    OpenAIRE

    Latorre Pellicer, Ana

    2014-01-01

    Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Bioquímica. Fecha de lectura: 26/06/2014 El DNA mitocondrial (mtDNA) es peculiar por muchos aspectos: es circular, extranuclear y poliploide, no tiene intrones, es transmitido uniparentalmente (no cumple las reglas mendelianas), no sufre recombinación y tiene su propio código genético. Si se valora la transcendencia del mtDNA por el tamaño genómico, podría ser considerado casi ...

  15. Sanidade, transmissão via semente e patogenicidade de fungos em sementes de Cedrela fissilis procedentes da região sul do Brasil

    OpenAIRE

    Marília Lazarotto; Marlove Fátima Brião Muniz; Rafael Beltrame; Álvaro Figueiredo dos Santos; Caciara Gonzatto Maciel; Solon Jonas Longhi

    2012-01-01

    http://dx.doi.org/10.5902/198050986617Pesquisas relacionadas à patologia de sementes de espécies florestais nativas, especialmente aquelas mais aprofundadas, são escassas. Assim, o objetivo deste estudo foi avaliar a sanidade das sementes de cedro através de dois métodos de detecção, avaliar a transmissão de fungos associados às sementes para as plântulas e avaliar a patogenicidade de fungos associados às sementes e transmitidos para as plântulas. Para tanto, sementes de seis procedências, de...

  16. La música como herramienta para la educación a través de los sentimientos

    OpenAIRE

    Cervantes-Fructuoso, Rosa

    2013-01-01

    La música se puede contemplar como herramienta para la educación y el aprendizaje interdisciplinar. Puesto que la educación en general y, en especial, la Educación Infantil consisten en la formación integral del ser humano, como persona, no cabe duda que los sentimientos forman una dimensión importante en la educación. Así la música provee de un medio de comunicación donde los sentimientos son la base del mensaje transmitido, otorgando al profesorado un ambiente educacional ideal para el desa...

  17. Efecto del extracto de paico (chenopodium ambrosioides), en parásitos gastrointestinales de gallos de pelea (gallus domesticus).

    OpenAIRE

    Paola Rodríguez; Elias Carvajal; Catalina Álavarez

    2011-01-01

    El uso empírico de las plantas como agentes de salud es ampliamente conocido en múltiplesculturas del mundo, conocimientos que son transmitidos de generación en generación. En la actualidad, las plantas medicinales y sus extractos, son utilizados con fines terapéuticos, instaurándose como una alternativa farmacológica, para diferentes patologías tanto en humanos como en animales. En la etnomedicina de algunas regiones de Latinoamérica, el paico (Chenopodium ambrosioides) ha sido empleado en i...

  18. Determinación de la capacidad de transmisión de fitoplasmas en dos morfoespecies de la familia Cicadellidae (Hemiptera: Auchenorryncha) de Bogotá D.C.

    OpenAIRE

    Perilla Henao, Laura Margarita

    2013-01-01

    Los fitoplasmas son bacterias fitopatógenas que carecen de pared celular, especializadas en la colonización intracelular del floema de plantas, son transmitidos por insectos vectores del suborden Auchenorryncha (Hemiptera) y están entre los seres vivos con menor tamaño. Los fitoplasmas forman un grupo monofilético en la clase Mollicutes que causan alteraciones morfológicas y fisiológicas en plantas ornamentales, hortícolas, maderables, frutales y cultivos en todo el mundo. No existen métodos...

  19. Detección de flavivirus en mosquitos (Diptera: Culicidae) de la Isla de Pascua-Chile

    OpenAIRE

    Ximena Collao; Lorena Prado; Christian González; Ana Vásquez; Romina Araki; Tuki Henríquez; Cindy Peña M

    2015-01-01

    Los flavivirus son virus transmitidos por artrópodos, principalmente por especies de mosquitos de los géneros Aedes y Culex (Culicidae) que se detectan principalmente en zonas tropicales y subtropicales. Los principales flavivirus de importancia en salud pública son el virus del dengue, del Nilo Occidental y fiebre amarilla, entre otros. En Chile continental no hay registro de flavivirus; sin embargo, se han detectado casos autóctonos de dengue en la Isla de Pascua desde el año 2002, al igual...

  20. Lenguaje y valores sociales en la publicidad impresa. Análisis de los valores manifiestos en el léxico de los anuncios de XL Semanal durante 2012

    OpenAIRE

    Gutiérrez Guerrero, María Dolores

    2015-01-01

    Esta tesis enmarcada dentro de las Ciencias de la Comunicación estudia los valores sociales presentes en el texto de los anuncios publicitarios. La novedad de la presente investigación es que analiza los valores transmitidos a través del léxico utilizado por la publicidad impresa. Mediante dicho análisis se determinan qué valores se utilizan y en qué medida. Como los medios de comunicación de masas son un factor de cambio social y los mensajes publicitarios que se emiten transmiten dete...

  1. La responsabilidad Social y jurídica ante el patrimonio bibliográfico

    Directory of Open Access Journals (Sweden)

    Pedraza García, Manuel José

    2010-07-01

    Full Text Available La legislación sobre patrimonio cultural establece el control necesario para determinar aquellos elementos culturales que deben conservados y transmitidos frente a los que no. La sociedad establece así el mecanismo de protección necesaria para la conservación futura de los bienes patrimoniales. Conceptos como coleccionismo de obras de arte y bibliofilia en el caso de los libros han estado presentes a lo largo de la historia y en buena medida han sido el germen de los actuales museos y bibliotecas nacionales

  2. Integración de secuencias discursivas. El caso de los mitos y leyendas de ascendencia maya en Chiapas (México), vinculadas a la creación del hombre y su entorno

    OpenAIRE

    Cruz Coutiño, José Antonio

    2008-01-01

    Ubica socio-etnográficamente a la provincia de Chiapas (México), con base en información estadística de los años 2005 a 2007; describe la cosmología y la cosmogonía de los mayas clásicos y posclásicos, y define al mito desde la lengua, la cultura y la ideología, pero sobre todo, caracteriza los relatos míticos tradicionales, transmitidos oralmente, como unidades discursivas, macroestructuras semánticas y macroactos de habla , de acuerdo con las propuestas metodológicas de Teun A. Van Dijk. S...

  3. La institucionalización de la maternología en España durante la Segunda República y el franquismo The institutionalization of maternology in Spain during Second Republic and the Franco period

    OpenAIRE

    Carmen COLMENAR ORZAES

    2013-01-01

    Desde principios del siglo XX se desarrolló en España una campaña de divulgación socioeducativa, dirigida a las mujeres, mediante la cual se les trató de inculcar que la misión principal de sus vidas, por su propia naturaleza, era la maternidad. Dicha campaña integraba un discurso médico, amparado por un Estado patriarcal, que culpabilizaba a las mujeres de la mortalidad infantil, a causa de su ignorancia en las prácticas de crianza. El mensaje transmitido, a través de diversos medios de difu...

  4. Métodos de representación en la pintura figurativa

    OpenAIRE

    Carroggio de Molina, Alberto, 1946-

    2012-01-01

    El conocido secretismo de los talleres y el desinterés de los pintores en dejar constancia escrita de su trabajo dificultan el estudio de la representación en la pintura. Esta situación obliga al pintor a recorrer personalmente paso a paso el mismo camino que sus antecesores. No existe más guía que las obras de los maestros y las máximas que, de taller en taller, se han transmitido hasta nosotros; es cierto que algunas son curiosas y con visos de verdad, pero en el fondo, poco aportan al pint...

  5. Mobile Web Best Practices 1.0 : basic guidelines

    OpenAIRE

    World Wide Web Consortium

    2008-01-01

    Este documento especifica las buenas prácticas para la entrega de contenidos Web para dispositivos móviles. El objetivo principal es mejorar la experiencia del usuario de la Web cuando accede desde este tipo de dispositivos. Las recomendaciones se refieren a los contenidos transmitidos y no a los procesos mediante los cuales se crea, ni a los dispositivos o las aplicaciones del usuario al que se entrega. Estas buenas prácticas están dirigidas principalmente a los creadores, mantenedores y ope...

  6. La responsabilidad Social y jurídica ante el patrimonio bibliográfico

    OpenAIRE

    Pedraza García, Manuel José, 1959-

    2010-01-01

    La legislación sobre patrimonio cultural establece el control necesario para determinar aquellos elementos culturales que deben conservados y transmitidos frente a los que no. La sociedad establece así el mecanismo de protección necesaria para la conservación futura de los bienes patrimoniales. Conceptos como coleccionismo de obras de arte y bibliofilia en el caso de los libros han estado presentes a lo largo de la historia y en buena medida han sido el germen de los actuales museos...

  7. El caso de los Vecinos Autoconvocados de Berazategui

    OpenAIRE

    García, Sandra

    2012-01-01

    El artículo analiza cómo a partir de la apropiación y el uso de Internet, por parte de la Asamblea de “Vecinos Autoconvocados por la Vida. No a la Subestación Rigolleau”, los agentes pudieron reconfigurar percepciones y prácticas de ciudadanía relacionadas específicamente a demandas por un medioambiente sano sin contaminación electromagnética, que no son transmitidos por los medios hegemónicos. De allí nuestro interés en proponer una lectura posible del discurso de estos actores, quienes a pa...

  8. El logo político como instrumento de significado en la comunicación electoral / The political logo as an instrument of significance in the electoral communication

    OpenAIRE

    David Fernández-Cañaveral

    2010-01-01

    El logo es una de las principales señas de identidad de cualquier compañía, producto, servicio o acontecimiento. Es la representación gráfica de su personalidad. Dentro de la comunicación electoral, un ámbito altamente persuasivo, el logo posee una presencia notable como firma visual del partido. La investigación que se presenta tiene como objetivo analizar los significados transmitidos por los atributos gráficos del logo político en las campañas electorales. Con este fin, se han analizado, e...

  9. Especificación en RSL de componentes basadas en streams

    OpenAIRE

    Riesco, Daniel Eduardo; Berón, Mario; Montejano, Germán Antonio; Dosch, Walter

    2003-01-01

    Un stream es una secuencia finita o infinita de mensajes transmitidos sobre un canal. Los streams tienen propiedades las cuales hacen posible modelar software en dominios específicos. RAISE es un método riguroso para el desarrollo de software. RSL, es el lenguaje de especificación formal usado por el método RAISE. Se han desarrollado herramientas que permiten verificar automáticamente las especificaciones de los módulos de software construidos. Aquí radica la ventaja del uso del método RAI...

  10. La conformación del señorío de Villatorcas (1668-1696)

    OpenAIRE

    Felipo Orts, Amparo

    2012-01-01

    En el contexto de la estrategia patrimonial y del proceso de promoción social del gobernador de Valencia don Basilio de Castellví y Ponce, esta comunicación analiza las circunstancias que acompañaron a la compra del lugar de Torcas (Castellón de la Plana) en 1668, así como las medidas que su insolvencia económica le exigió adoptar para afrontar su pago. Transmitido a su hijo, don José de Castellví y Alagón, marqués de Villatorcas, el nuevo propietario llevaría a cabo una destacada...

  11. SADA-Sat : Sistema de Adquisición y Almacenamiento de Datos Ambientales por Protocolo SDI-12 con transmisión satelital

    OpenAIRE

    Díaz, Bruno; Díaz, Emanuel; Pereyra, Hugo; Mercado, Gustavo; Diedrichs, Ana

    2014-01-01

    El artículo describe el diseño e implementación de un sistema de adquisición automática de datos ambientales cuyos datos son transmitidos por comunicación satelital. El sistema ha sido desarrollado para su uso en ambientes agrestes, de difícil acceso y con escasos medios de comunicación tradicional. Los datos ambientales se digitalizan a través de un circuito específico cercano al sensor y se envían mediante el protocolo bus serie inspirado en SDI-12 a la plataforma colectora de datos (DCP...

  12. La universalidad de los valores a través de los cuentos en Educación Infantil

    OpenAIRE

    Curiel Carazo, María Zoe

    2015-01-01

    El cuento es un recurso educativo muy útil para trabajar diversas áreas y contenidos en Educación Infantil y entre ellos está la Educación en Valores. Los cuentos, y en especial los cuentos tradicionales, han transmitido valores a lo largo de generaciones. En este trabajo vamos a abordar la Educación en Valores a través de cuentos tradicionales, con un valor añadido, que usaremos cuentos procedentes de diferentes culturas de la Unión Europea para apreciar la universalidad de lo...

  13. Ideal de belleza femenino en los medios de comunicación y alteraciones de la conducta alimentaria. Influencia y posibles soluciones

    OpenAIRE

    Sánchez-Carracedo, David; López-Guimerà, Gemma; Fauquet Ars, Jordi

    2014-01-01

    La investigación ha mostrado que la exposición constante a mensajes e imágenes relacionadas con el ideal de belleza femenino, el control del peso y la figura corporal transmitidos por los medios de comunicación, es un factor de riesgo bien establecido para el desarrollo de insatisfacción corporal, las preocupaciones por el peso, el inicio de conductas no saludables de control del peso y los trastornos de la conducta alimentaria en mujeres. En esta comunicación se presentarán los resultados de...

  14. ¿Acaso basta traducirse para entenderse?1 Un ejemplo de inconsciente de escuela: el Greenwood Dictionary of World History

    OpenAIRE

    Christin, Olivier

    2014-01-01

    La traducción es un instrumento irremplazable para el intercambio de ideas en muchos aspectos de la comunicación humana. Sin embargo, existen factores que pueden interferir para que el mensaje original no sea cabalmente transmitido. Aunado a ello, el uso del inglés como lengua obligada en el ámbito académico, puede provocar variaciones en los matices que pueden llegar a reducir el significado de conceptos originalmente complejos. Democracia por ejemplo, puede entenderse de maneras distintas a...

  15. Multimedia Educativa para apoyar el Proceso de Enseñanza-Aprendizaje en la asignatura Comunicación Organizacional

    OpenAIRE

    Ordanis Nerza González Agüero

    2008-01-01

    Aborda lo referido al diseño de una multimedia educativa y sus indicaciones metodológicas, para favorecer el proceso de enseñanza - aprendizaje de la asignatura Comunicación Organizacional de la Carrera Comunicación Social, la que integra los contenidos de la asignatura y los presenta de una forma amena valiéndose de diversos recursos multimediales, lo que le facilita a los estudiantes la aprensión de los conocimientos transmitidos. Su concepción ha sido sustentada por el Proceso Unificado de...

  16. Detection and quantification of potato mop-top virus (pmtv) in colombia using qrt-pcr

    OpenAIRE

    García Bastidas, Nevar Alirio; Gutiérrez Sánchez, Pablo Andrés; Marín Montoya, Mauricio Alejandro

    2014-01-01

    El Potato mop-top virus (PMTV) es uno de los virus re-emergentes en cultivos de papa en Colombia. Es transmitido por Spongospora subterranea, el agente causal de la sarna polvosa. La detección del PMTV presenta dificultades debido a su distribución irregular en las plantas, bajo título y movimiento sistémico como ARN desnudo. Con el fin de ampliar el rango de herramientas disponibles para detectar el PMTV en los programas de certificación de tubérculo-semilla, en este estudio se evaluó la pru...

  17. Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR

    OpenAIRE

    Nevar García Bastidas; Pablo Gutiérrez Sánchez; Mauricio Marín Montoya

    2013-01-01

    El Potato mop-top virus (PMTV) es uno de los virus re-emergentes en cultivos de papa en Colombia. Es transmitido por Spongospora subterranea, el agente causal de la sarna polvosa. La detección del PMTV presenta dificultades debido a su distribución irregular en las plantas, bajo título y movimiento sistémico como ARN desnudo. Con el fin de ampliar el rango de herramientas disponibles para detectar el PMTV en los programas de certificación de tubérculo-semilla, en este estudio se evaluó la pru...

  18. Sensibilidad a fungicidas de las especies de Fusarium responsables de la pudrición de la mazorca de maíz.

    OpenAIRE

    Pérez Rodríguez, Jaime

    2014-01-01

    Una de las enfermedades más importantes en México y en todos los países donde se cultiva maíz, es la pudrición de la mazorca. Además de reducir el rendimiento del cultivo, esta enfermedad afecta las cualidades físicas, fisiológicas y fitosanitarias de las semillas. Los agentes causales reportados responsables de la pudrición de la mazorca, corresponden al género Fusarium (hongos transmitidos por semilla). Una forma fácil y accesible de manejar a estos patógenos es con el uso de fungicidas apl...

  19. En búsqueda de un cuidado universal y cultural Finding a Universal and cultural care

    OpenAIRE

    Cecilia Rohrbach

    2007-01-01

    La responsabilidad analítica que cada uno de nosotros tiene, se hace presente cada vez que se elabora una teoría relativa al cuidado, ya que construir y confirmar, o invalidar postulados, contribuye al avance de la ciencia. La crítica, en su sentido original de analizar, es también parte de la ciencia, al confirmarla enriqueciendo legítimamente su conocimiento y aplicación. El cuidado transcultural, creado y transmitido principalmente por enfermeras americanas, se origina en la antropología a...

  20. Implementación en VHDL de generadores de números pseudo-aleatorios (PRNG'S) criptográficamente seguros

    OpenAIRE

    Grau Segura, Manuel

    2009-01-01

    La tecnología RFID está atrayendo un enorme interés y se ha convertido en una tecnología con un despliegue y crecimiento muy amplio y elevado, debido a la enorme importancia que están cobrando los Sistemas de Autentificación Automáticos. La gran ventaja de esta tecnología radica en que transmite la información sin necesidad de contacto físico, los datos son transmitidos por radio frecuencia entre un transmisor (Transponder) y un lector. El principal problema de esta tecnología es la necesidad...

  1. Generación de cuadrados latinos de orden 256 utilizando un grafo de reemplazos

    OpenAIRE

    Gallego Sagastume, Ignacio

    2015-01-01

    Los cuadrados Latinos (LSs) son estructuras algebraicas con aplicaciones en criptografía. Si los LSs son aleatorios y uniformemente distribuidos, pueden ser usados como claves para algoritmos de encriptación simétricos. En el contexto de un protocolo de comunicación seguro, debe generarse un nuevo LS cada cierta cantidad de tiempo o cantidad de datos transmitida para no correr el riesgo de que un atacante lo deduzca y pueda así descifrar los mensajes transmitidos. El tiempo y recursos requeri...

  2. Miocarditis por dengue

    OpenAIRE

    Ricardo Amador García Hernández; Fidel Espinosa Rivera; Lianet Rivero Seriel

    2013-01-01

    El dengue es un arbovirus transmitido por el Aedes aegypti, produce los cuadros clínicos de dengue clásico, dengue hemorrágico y síndrome de choque por dengue, aisladamente se reportan casos de miocarditis. Se presentó un paciente con antecedentes de dengue clásico que manifestó dolor precordial asociado a cambios electrocardiográficos y fue ingresado en este hospital por síndrome coronario agudo probable, al cual se le diagnosticó clínicamente miocarditis por dengue.

  3. Incidencias del Sistema Global de la Información Electrónica en el periodismo contemporáneo

    OpenAIRE

    Flores Vivar, Jesús Miguel

    1999-01-01

    La implantación de Internet y los Sistemas de Información dentro de los medios, está concibiendo una nueva forma de trabajo en el entorno periodístico. Por un lado, los Sistemas de Información permiten homogeneizar y optimizar la información que la propia empresa produce, estableciéndose, un continuo flujo de conocimientos. Estos conocimientos, a su vez, son transmitidos a los usuarios, componentes de la Sociedad de la información, a través de las redes telemáticas. El resultado deriva en el ...

  4. La interacción de los códigos en doblaje: juegos de palabras y restricciones visuales

    OpenAIRE

    Martínez Tejerina, Anjana

    2012-01-01

    El siguiente artículo profundiza en la complejidad del trasvase del humor, basándose en un problema recurrente en el doblaje de comedias: los juegos de palabras transmitidos parcialmente a través de la imagen. El estudio analiza los factores que inciden en su traducción e ilustra las distintas técnicas de traducción con escenas de la filmografía de los Hermanos Marx. Finalmente, muestra las conclusiones obtenidas a partir del análisis del doblaje de 74 juegos de palabras con restricción visua...

  5. Caracterização da intervenção do treinador na reunião de preparação da equipa para a competição no Futebol : Estudo comparativo de treinadores da 1ª Liga e da 2ª Divisão-B no Escalão de Seniores Masculinos

    OpenAIRE

    Pacheco, Rui Manuel de Gouveia

    2002-01-01

    Pretendemos neste estudo caracterizar os conteúdos que são transmitidos pelos treinadores aos jogadores, na reunião de preparação da equipa para a competição no futebol, no escalão de seniores masculinos. Realizamos um estudo comparativo partindo de uma amostra constituida por seis treinadores pertencentes a equipas classificadas no terço superior do campeonato nacional de futebol da 1ª liga e seis treinadores pertencentes a equipas que disputam os lugares cimeiros do campeonato nacional de f...

  6. La publicidad de moda dirigida a los jóvenes: ¡mírame a los ojos!, la persuasión silenciosa

    OpenAIRE

    Figueiredo Pina, Helena

    2014-01-01

    La presente investigación tiene como objetivo ofrecer una clara contribución al estudio de la problemática sobre los efectos que tienen en los jóvenes las imágenes de los medios de comunicación. Los ideales de belleza y los valores transmitidos por la cultura de los medios de comunicación tienen consecuencias sociales importantes, especialmente en lo que dice respecto al autoconcepto y a la imagen corporal de los adolescentes y jóvenes adultos, creando grandes discrepancias entre el “self” ac...

  7. Modernidad y contextualidad regional : el caso de Emilio Duhart

    OpenAIRE

    Camplá Lehmann, José R.

    2013-01-01

    Es recurrente lo transmitido en la mayoría de los escritos y por los estudiosos, en el sentido de que el Movimiento Moderno tanto en Latinoamérica como particularmente en Chile, ha sido un movimiento de carácter exógeno, con un alto contenido de desarraigo, sin considerar factores sociales, culturales ni territoriales y que en nuestro país ha sido impuesto como un acto de imitación sin más. Esta tesis pretende dar a conocer a través de la muestra de algunas obras realizadas desde las décadas ...

  8. Sobre um projeto extracurricular de matemática para a pré-escolar e o primeiro ciclo no ensino básico

    OpenAIRE

    Videira, Maria Adelaide Duarte Carreira Leite

    2012-01-01

    Tese de doutoramento em Ciências (área de especialização em Matemática) A premissa central na base deste trabalho assenta no princípio da conveniência – melhor, na convicção da necessidade – de se começar, desde muito cedo, isto é, desde os 5/6 anos, a estimular na mente infantil o pensamento lógico-abstrato, por via de conceitos e algoritmos matemáticos, transmitidos da mesma forma natural pela qual os juvenis de diferentes espécies animais adquirem os comportamentos e as técn...

  9. Utilização segura da internet como recurso educativo na prática de ensino supervisionada

    OpenAIRE

    Gil, Henrique; Félix, Carina

    2015-01-01

    No âmbito da Prática de Ensino Supervisionada no 1.º CEB foi realizada uma investigação qualitativa com o objetivo de consciencializar os alunos para os perigos na utilização da internet. Foram implementadas atividades onde, para além dos conteúdos explorados, foram transmitidos as principais cuidados para urna navegação segura na internet. Na investigação foram também envolvidos os pais e Os professores do agrupamento de escolas do 1.° CEB no sentido de recolher opiniões acerca da importânci...

  10. Microorganismos indicadores de la calidad del agua potable en cuba

    OpenAIRE

    Marlen Robert Pullés

    2014-01-01

    Los agentes patógenos transmitidos por el agua constituyen un problema mundial que demanda un urgente control. Las bacterias, virus y parásitos causan enfermedades que varían en severidad. La determinación de microorganismos en el agua de consumo y su concentración proporcionan herramientas de control, indispensables para la toma de decisiones. Los controles rutinarios de todos los microorganismos, potencialmente riesgosos para la salud, resultan difíciles de llevar a cabo, debido a que ello ...

  11. Las estructuras

    OpenAIRE

    Alarcón Álvarez, Enrique

    2001-01-01

    La afirmación de que las primeras realizaciones arquitectónicas y de ingeniería estructural son tan antiguas como la civilización no es gratuita. En efecto, tan pronto como se desea cubrir un espacio, salvar un vano o transmitir un empuje (vertical u horizontal), es preciso el desarrollo de un conocimiento específico. El dominio de las técnicas para resolver,en particular, tales problemas ha sido transmitido a través de construcciones religiosas o áulicas que han permanecido a través de los s...

  12. Integración de servicios audiovisuales de TV terrestre e IPTV

    OpenAIRE

    Seifert, Santiago

    2015-01-01

    Este trabajo busca el aumento de la lista de servicios provista por la televisión digital terrestre. El objetivo es extender la norma ISDB-Tb (utilizada en Argentina) para la inclusión de servicios audiovisuales transmitidos por red IP. Con esto, se combina la robustez de la emisión broadcast por radiofrecuencia y la flexibilidad de IPTV. El resultado es un sistema híbrido con una lista de servicios con tamaño comparable a la televisión analógica por cable, con la confiabilidad, la calidad y ...

  13. Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment

    International Nuclear Information System (INIS)

    Crimean-Congo hemorrhagic fever (CCHF) virus is highly pathogenic for humans and remains the only Category A virus for which full sequence information is currently unavailable. In this study we completed CCHF genome characterization by determining the L segment sequence using Dugbe and CCHF virus-specific oligonucleotides. Sequence alignments revealed the presence of four previously described conserved regions in all Bunyaviridae polymerases. Interestingly, additional regions containing putative Ovarian Tumor (OTU)-like cysteine protease and helicase domains were identified in the L segments of CCHF and Dugbe viruses, suggesting an autoproteolytic cleavage process for nairovirus L proteins

  14. Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran

    OpenAIRE

    Sadegh Chinikar; Nariman Shah-Hosseini; Saeid Bouzari; MohammadAli Shokrgozar; Ehsan Mostafavi; Tahmineh Jalali; Sahar Khakifirouz; Groschup, Martin H; Matthias Niedrig

    2015-01-01

     Background: Crimean-Congo Hemorrhagic Fever Virus (CCHFV) belongs to genus Nairovirus and family Bunyaviridae. The main aim of this study was to investigate the extent of recombination in S-segment genome of CCHFV in Iran.Methods: Samples were isolated from Iranian patients and those available in GenBank, and analyzed by phyloge­netic and bootscan methods.Results: Through comparison of the phylogenetic trees based on full length sequences and partial fragments in the S-segment genome of CCHF...

  15. Radiological Findings and their Clinical Correlations in Nephropathia Epidemica

    Energy Technology Data Exchange (ETDEWEB)

    Paakkala, A.; Mustonen, J. [Medical School, Univ. of Tampere, and Dept. of Radiology and Internal Medicine, Tampere Univ. Hospital, Tampere (Finland)

    2007-04-15

    Nephropathia epidemica (NE) is a mild form of hemorrhagic fever with renal syndrome (HFRS). Its course varies from asymptomatic to fatal. The etiologic agent, Puumala virus, belongs to the Hantavirus genus of the Bunyaviridae family. Respiratory symptoms, from common cold to respiratory distress, occur in NE. Acute renal failure (ARF) is evident in over 90% of hospital-treated NE patients. In this review article, special attention is paid to radiological lung and renal involvement to investigate the occurrence and type of manifestations during the acute phase of infection and recovery.

  16. La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys

    OpenAIRE

    Murphy Brian R; Firestone Cai-Yen; Ward Jerrold M; Cress Christina M; Bennett Richard S; Whitehead Stephen S

    2008-01-01

    Abstract Background La Crosse virus (LACV), family Bunyaviridae, was first identified as a human pathogen in 1960 after its isolation from a 4 year-old girl with fatal encephalitis in La Crosse, Wisconsin. LACV is a major cause of pediatric encephalitis in North America and infects up to 300,000 persons each year of which 70–130 result in severe disease of the central nervous system (CNS). As an initial step in the establishment of useful animal models to support vaccine development, we exami...

  17. The role of platelets in the pathogenesis of viral hemorrhagic fevers.

    Directory of Open Access Journals (Sweden)

    Juan C Zapata

    2014-06-01

    Full Text Available Viral hemorrhagic fevers (VHF are acute zoonotic diseases that, early on, seem to cause platelet destruction or dysfunction. Here we present the four major ways viruses affect platelet development and function and new evidence of molecular factors that are preferentially induced by the more pathogenic members of the families Flaviviridae, Bunyaviridae, Arenaviridae, and Filoviridae. A systematic search was performed through the main medical electronic databases using as parameters all current findings concerning platelets in VHF. Additionally, the review contains information from conference proceedings.

  18. Radiological Findings and their Clinical Correlations in Nephropathia Epidemica

    International Nuclear Information System (INIS)

    Nephropathia epidemica (NE) is a mild form of hemorrhagic fever with renal syndrome (HFRS). Its course varies from asymptomatic to fatal. The etiologic agent, Puumala virus, belongs to the Hantavirus genus of the Bunyaviridae family. Respiratory symptoms, from common cold to respiratory distress, occur in NE. Acute renal failure (ARF) is evident in over 90% of hospital-treated NE patients. In this review article, special attention is paid to radiological lung and renal involvement to investigate the occurrence and type of manifestations during the acute phase of infection and recovery

  19. The 3′ Untranslated Region of the Andes Hantavirus Small mRNA Functionally Replaces the Poly(A) Tail and Stimulates Cap-Dependent Translation Initiation from the Viral mRNA ▿

    OpenAIRE

    Vera-Otarola, Jorge; Soto-Rifo, Ricardo; Ricci, Emiliano P.; Ohlmann, Théophile; Darlix, Jean-Luc; López-Lastra, Marcelo

    2010-01-01

    In the process of translation of eukaryotic mRNAs, the 5′ cap and the 3′ poly(A) tail interact synergistically to stimulate protein synthesis. Unlike its cellular counterparts, the small mRNA (SmRNA) of Andes hantavirus (ANDV), a member of the Bunyaviridae, lacks a 3′ poly(A) tail. Here we report that the 3′ untranslated region (3′UTR) of the ANDV SmRNA functionally replaces a poly(A) tail and synergistically stimulates cap-dependent translation initiation from the viral mRNA. Stimulation of ...

  20. The Presence and Seroprevalence of Arthropod-Borne Viruses in Nasiriyah Governorate, Southern Iraq: A Cross-Sectional Study.

    Science.gov (United States)

    Barakat, Ali Mohammed; Smura, Teemu; Kuivanen, Suvi; Huhtamo, Eili; Kurkela, Satu; Putkuri, Niina; Hasony, Hassan J; Al-Hello, Haider; Vapalahti, Olli

    2016-04-01

    The knowledge on the presence and seroprevalence of arboviruses in Iraq is fragmental. To assess the exposure of the population to arbovirus infections in southern Iraq, we conducted a serological screening of the most common arbovirus groups using immunofluorescence, hemagglutination inhibition and neutralization tests. Serum samples of 399 adult volunteers were collected in Nasiriyah, Iraq. Antibodies were detected against West Nile virus (WNV) (11.6%), sandfly-borne Sicilian virus serocomplex (18.2%), sandfly-borne Naples virus serocomplex (7.8%), Sindbis virus (1.5%), chikungunya virus (0.5%), and Tahyna virus (2.0%). The results suggest that WNV and sandfly-borne phlebovirus infections are common in southern Iraq, and these viruses should be considered as potential causative agents in patients with febrile disease and/or neurological manifestations. PMID:26880770

  1. Co-circulation of Toscana virus and Punique virus in northern Tunisia: a microneutralisation-based seroprevalence study.

    Directory of Open Access Journals (Sweden)

    Sonia Sakhria

    Full Text Available BACKGROUND: In northern Tunisia, the co-circulation of two related sand fly-borne phleboviruses, Toscana virus (TOSV and Punique virus (PUNV was previously demonstrated. In contrast to TOSV, a prominent human pathogen, there is no data supporting that PUNV is capable to infect and cause disease to humans. We studied the respective involvement of TOSV and PUNV in human infections in northern Tunisia through a seroprevalence study. METHODS: The presence of TOSV and PUNV neutralising antibodies (NT-Ab was tested in human sera collected from 5 districts of the governorate of Bizerte, and the titres of NT-Ab were estimated by microneutralisation (MN assay. PRINCIPAL FINDINGS: A total of 1,273 sera were processed. TOSV and PUNV NT-Ab were detected in 522 (41% and 111 sera (8.72% respectively. TOSV seroprevalence varied from 17.2% to 59.4% depending on the district. Analysis of TOSV geometric mean titre values demonstrated a constant increase according to the age. The vast majority of sera containing NT-Ab were found to be more reactive toward TOSV than PUNV. Indeed, past infections with PUNV and TOSV were undisputable for 5 and 414 sera, respectively. CONCLUSIONS: PUNV may be capable to infect humans but at a low rate. TOSV is responsible for the vast majority of human infections by sand fly-borne phleboviruses in northern Tunisia. TOSV must be considered by physician and tested in diagnostic laboratories for patients with meningitis and unexplained fever in northern Tunisia.

  2. Ecological studies of enzootic Venezuelan equine encephalitis in north-central Venezuela, 1997-1998.

    Science.gov (United States)

    Salas, R A; Garcia, C Z; Liria, J; Barrera, R; Navarro, J C; Medina, G; Vasquez, C; Fernandez, Z; Weaver, S C

    2001-01-01

    From 1997-1998, we investigated the possible continuous circulation of epizootic Venezuelan equine encephalitis (VEE) virus suggested by a 1983 subtype IC interepizootic mosquito isolate made in Panaquire, Miranda State, Venezuela. The study area was originally covered by lowland tropical rainforest but has been converted into cacao plantations. Sentinel hamsters, small mammal trapping, mosquito collections, and human serosurveys were used to detect active or recent virus circulation. Six strains of subtype ID VEE virus were isolated from hamsters that displayed no apparent disease. Four other arboviruses belonging to group A (Togaviridae: Alphavirus), two Bunyamwera group (Bunyaviridae), and three Gamboa group (Bunyaviridae) arboviruses were also isolated from hamsters, as well as 8 unidentified viruses. Venezuelan equine encephalitis-specific antibodies were detected in 5 small mammal species: Proechimys guairae, Marmosa spp., and Didelphis marsupialis. Mosquito collections comprised of 38 different species, including 8 members of the subgenus Culex (Melanoconion), did not yield any virus isolates. Sera from 195 humans, either workers in the cacao plantation or nearby residents, were all negative for VEE virus antibodies. Sequences of 1,677 nucleotides from the P62 gene of 2 virus isolates indicated that they represent a subtype ID lineage that is distinct from all others characterized previously, and are unrelated to epizootic VEE emergence. PMID:11425168

  3. Purification, crystallization and preliminary X-ray crystallographic analysis of the nucleocapsid protein of Bunyamwera virus

    International Nuclear Information System (INIS)

    The nucleocapsid protein of Bunyamwera virus, the prototypic member of the Bunyaviridae family of segmented negative-sense RNA viruses, has been expressed and crystallized. Complete X-ray diffraction data sets have been collected. Bunyamwera virus (BUNV) is the prototypic member of the Bunyaviridae family of segmented negative-sense RNA viruses. The BUNV nucleocapsid protein has been cloned and expressed in Escherichia coli. The purified protein has been crystallized and a complete data set has been collected to 3.3 Å resolution at a synchrotron source. Crystals of the nucleocapsid protein belong to space group C2, with unit-cell parameters a = 384.7, b = 89.8, c = 89.2 Å, β = 94.4°. Self-rotation function analysis of the X-ray diffraction data has provided insight into the oligomeric state of the protein as well as the orientation of the oligomers in the asymmetric unit. The structure determination of the protein is ongoing

  4. Distribuição espacial da meleira do mamoeiro em zonas de trópico úmido e trópico semi-árido Spatial patterns of papaya sticky disease in brazilian semi arid and rain forest regions

    OpenAIRE

    Carlos A. Vidal; Francisco F. Laranjeira; Antônio S. Nascimento; Tuffi C. Habibe

    2004-01-01

    O vírus da meleira, transmitido por Bemisia tabaci, é um dos maiores problemas da cultura do mamoeiro (Carica papaya), sendo responsável por perdas de até 100% na produção. Com o objetivo de compreender melhor sua epidemiologia e gerar subsídios para estudos da influência de fatores culturais e bioecológicos na dinâmica da doença, avaliou-se a distribuição espacial de plantas afetadas pela meleira em zonas de Trópico Úmido e Trópico Semi-árido, em 15 plantios comerciais em Eunápolis-BA e Petr...

  5. Viajes del andalusí Ibn Ŷubayr al Oriente

    Directory of Open Access Journals (Sweden)

    Maíllo Salgado, Felipe

    2005-04-01

    Full Text Available La Rihla o relación de viaje del andalusí Ibn Ŷubayr es la obra maestra de ese género en las letras árabes. Nadie ha transmitido un cuadro tan vivo y acabado de los principales centros del oriente árabe, ni ha dejado descripciones más precisas de los lugares visitados, ni ha evocado de modo tan personal los hechos y los gestos de los hombres. Es también documento esencial para la arqueología islámica, pues tiene especial cuidado en describir y situar los principales monumentos de las ciudades, adobado todo con datos de tipo geográfico, histórico, etnológico, folclórico...; nada importante escapa a su mirada.…

  6. Anorexia nervosa e transmissão psíquica transgeracional

    Directory of Open Access Journals (Sweden)

    Élide Dezoti Valdanha

    2013-03-01

    Full Text Available O estudo investigou a transmissão psíquica em três gerações de mulheres de uma família - avó, mãe e filha, esta última com diagnóstico de anorexia nervosa. Utilizou-se como estratégia metodológica o estudo de caso familiar e o enfoque da transmissão psíquica para análise dos dados. Perceberam-se dificuldades e sofrimentos decorrentes de conteúdos psíquicos transmitidos sem elaboração na sucessão intergeracional, fomentando relações truncadas e conflagradas, veladas por segredos familiares e empobrecimento subjetivo.

  7. CRISIS FINANCIERA MUNDIAL: IMPACTO EN LA ECONOMÍA MEXICANA

    OpenAIRE

    Hector Ruiz Ramirez

    2010-01-01

    A finales del año 2008, la economía mexicana empieza a sentir los efectos de la crisis en la economía norteamericana, que se expandió a la mayoría de países y que ante la falta de regulación, se extiende rápidamente. Esta falta de regulación ha iniciado en la actualidad un interesante debate sobre el tema. El ciclo recesivo de Estados Unidos es transmitido de manera más o menos inmediata a la economía mexicana, a través de tres vías: exportaciones, remesas familiares, e inversión extranjera d...

  8. Dengue : etiologia, patogénese e suas implicações a nível global

    OpenAIRE

    Nunes, Juliana da Silva

    2011-01-01

    A doença causada pelo vírus do Dengue tornou-se uma das principais doenças infecciosas emergentes a nível mundial, sendo agora considerada uma epidemia global e registada em mais de 120 países. Tal facto põe em risco grande parte da população mundial. O vírus do Dengue pertence ao grupo das arboviroses, referente a família Flaviviridae, género Flavivírus, possui quatro serotipos geneticamente e antigenicamente distintos (1-4). Transmitido ao homem através da picada de mosquitos hematófa...

  9. A vivência em uma instituição de longa permanência

    OpenAIRE

    Michel, Tatiane

    2011-01-01

    Resumo:As Instituições de Longa Permanência para Idosos (ILPI) são locais para residência coletiva, nas quais pessoas com idade avançada buscam a proteção e o amparo que, frequentemente, não encontraram no seu ambiente familiar e social. O ambiente de convivência familiar abrange um conjunto de hábitos e valores, transmitidos de geração em geração e que se refletem no cotidiano das pessoas. Deste modo, quando idosos são encaminhados para viver em ILPI, correm um risco de maior isolamento e in...

  10. Desempenho de rede de sensores sem fio em casa de vegetação Performance of wireless sensor network in a greenhouse

    OpenAIRE

    Barbara Teruel; Elaine C. de S. Alves; Clerivaldo J. Roccia; Francisco J. Arnold; Leonardo L. Bravo-Roger

    2012-01-01

    A potência de sinal é um importante parâmetro a ser considerado em um projeto de implantação de rede de sensores sem fio no meio agrícola. Este trabalho tem como objetivo coletar dados de qualidade de sinal transmitido entre uma estação base e um nó sensor situado no interior de uma casa de vegetação, localizada no campo experimental da Faculdade de Engenharia Agrícola - UNICAMP, com o auxílio de ferramentas computacionais. Após a implantação do nó sensor foram realizados experimentos com vis...

  11. El rigor: garantía para los periodistas, credibilidad para los medios y esperanza para los ciudadanos

    Directory of Open Access Journals (Sweden)

    Jesús Erney Torres Loaiza

    2004-01-01

    Full Text Available El artículo plantea un análisis profundo sobre la necesidad del rigor en la información y la verificación de las fuentes. Según el autor, no basta que un periodista cubra la información con la premisa de divulgar la verdad, es necesario que a la hora de publicarla tenga presente que el rigor es fundamental. La advertencia de cómo se consiguió la información y el contexto de los hechos, deben ser transmitidos al público, sobretodo en los casos donde está de por medio la condición humana, porque al omitir estos detalles el periodista caería en la desinformación y no estaría entregando integralmente la noticia.

  12. Diagnóstico laboratorial de casos de importação de infeções por vírus Zika

    OpenAIRE

    Alves, Maria João; Zé-Zé, Líbia

    2016-01-01

    O vírus Zika (Flavivirus) é um arbovírus transmitido sobretudo por mosquitos, mas também, por transmissão materno-fetal e sexual. Existem evidências que as infeções por vírus Zika podem estar associadas à síndrome de Guillian-Barré e a casos congénitos de microcefalia e outras malformações do sistema nervoso central. As infeções por vírus Zika, Dengue e Chikungunya partilham, atualmente, os mosquitos vetores, a sintomatologia e a distribuição geográfica. O Centro de Estudos ...

  13. Epidemiologia e estudo entomológico dos potenciais vectores do vírus da língua azul na região do Vale do Tejo

    OpenAIRE

    Rodrigues, Luís Filipe Gonçalves

    2008-01-01

    A Língua Azul (LA) é causada por um vírus RNA da familia Reoviridae e do género Orbivirus e existem 24 serótipos. É uma doença infecciosa não contagiosa, sendo o vírus transmitido por insectos culicóides e afecta espécies animais ruminantes domésticas e selvagens. É também designada por Febre Catarral Ovina, pois a maioria dos serótipos normalmente causa doença clínica unicamente em ovinos. Desde 2006 foi identificado na Europa o serótipo 8 do vírus da LA, que também afecta cli...

  14. Epidemiología molecular de la fiebre amarilla en Colombia: análisis filogenético

    OpenAIRE

    Jorge Boshell; Calvache, D.; Bernal, M.P.; Méndez, Jairo A

    2004-01-01

    El virus de la fiebre amarilla pertenece a la familia Flaviviridae, género Flavivirus, y es el responsable de una enfermedad hemorrágica aguda que aún hoy en día afecta a más de 200.000 personas al año en regiones tropicales de África y Suramérica. El virus puede ser transmitido de un humano infectado a un humano susceptible por el mosquito doméstico Aedes aegypti o de primates no humanos a primates humanos por...

  15. A amamentação nos manuais escolares de estudo do meio do 1º ciclo do ensino básico La lactancia en los libros de texto escolares del Estudio del Medio del 1º ciclo de la educación básica Breastfeeding in Environmental Studies textbooks used in the first cycle of basic education

    OpenAIRE

    Dulce Maria Pereira Garcia Galvão; Isília Aparecida da Silva

    2011-01-01

    Para conhecer como a amamentação é abordada às crianças do 1º Ciclo, e o que aprendem na escola sobre este tema, analisaram-se, do 1º ao 4º ano de escolaridade, 19 manuais escolares de Estudo do Meio, adoptados em 73 escolas públicas de seis agrupamentos de escolas da área educativa de Coimbra. Na análise dos conceitos transmitidos definiram-se, a priori, alguns considerados imprescindíveis para ensinar e explicar à criança noções básicas de amamentação. Apenas dois manuais do 3º ano incluíam...

  16. Relatório de estágio em terapia manual ortopédica

    OpenAIRE

    Marques, Rui

    2010-01-01

    pacientes ao longo dos anos. Especialmente na área de músculo-esquelético e terapia manual, há uma panóplia de técnicas e métodos ao dispor dos Fisioterapeutas. Com todas estas técnicas, o Fisioterapeuta tem que saber seleccioná-las para cada situação clínica que lhe surge, na tentativa de obter os melhores resultados possíveis na reabilitação do paciente. Este trabalho destina-se a elaborar um relatório de estágio, onde vão ser abordados os conhecimentos transmitidos e adqu...

  17. As deficiências auditivas relacionadas às alterações do DNA mitocondrial.

    OpenAIRE

    Carvalho Maria F. P. de; Ribeiro Fernando A. Quintanilha

    2002-01-01

    A deficiência auditiva é sintoma comum que pode apresentar várias etiologias, entre elas as causadas por alterações genéticas. As mutações genéticas podem ocorrer em genes nucleares e mitocondriais. A mitocôndria, uma organela intracelular, tem o seu próprio genoma (DNA), que é uma molécula circular e é transmitido exclusivamente pela mãe. As mutações do DNA mitocondrial são transmitidas pela linhagem materna, mas podem ocorrer mutações espontâneas. O fenótipo, ou expressão clínica, da mutaçã...

  18. As deficiências auditivas relacionadas às alterações do DNA mitocondrial. Hearing loss related to mitochondrial DNA changes

    OpenAIRE

    Maria F. P. de Carvalho; Fernando A. Quintanilha Ribeiro

    2002-01-01

    A deficiência auditiva é sintoma comum que pode apresentar várias etiologias, entre elas as causadas por alterações genéticas. As mutações genéticas podem ocorrer em genes nucleares e mitocondriais. A mitocôndria, uma organela intracelular, tem o seu próprio genoma (DNA), que é uma molécula circular e é transmitido exclusivamente pela mãe. As mutações do DNA mitocondrial são transmitidas pela linhagem materna, mas podem ocorrer mutações espontâneas. O fenótipo, ou expressão clínica, da mutaçã...

  19. Rastreio de leishmania infantum em cães assintomáticos na região de Mafra

    OpenAIRE

    Armés, Sílvia Maria Martins Curado

    2010-01-01

    A leishmaniose canina é uma doença parasitária de distribuição mundial provocada por um protozoário, que em Portugal, pertence à espécie L. infantum e é transmitido por vectores do género Phlebotomus. Nas zonas endémicas os cães infectados com L. infantum e assintomáticos são infectantes para o flebótomo vector numa proporção similar aos sintomáticos, contudo a comunidade veterinária não está devidamente alertada para a sua existência e importância. Este trabalho contribui para realçar a i...

  20. Las empresas multinivel como modelo distópico: estudio de representaciones sociales

    Directory of Open Access Journals (Sweden)

    Mónica Maisterrena González

    2014-07-01

    Full Text Available El presente estudio muestra las estrategias utilizadas por las empresas multinivel para ejercer un esquema homogéneo de representaciones sociales entre los asociados de las mismas. Se ha determinado la inevitabilidad de mantener un control "ideológico” sobre los asociados para el correcto funcionamiento de la empresa. La dinámica empresarial de estas compañías propone un modelo de satisfacción personal que debe ser transmitido y no puede ser cuestionado y que, por tanto, violenta la individualidad de los asociados. Se han utilizado, a manera de analogía, las novelas 1984 de George Orwell y Un mundo feliz de Aldous Huxley como modelos de una sociedad distópica que han sido llevadas a la práctica por las empresas multinivel.

  1. Relação entre apresentação clínica, carga viral e a titulação de anticorpos na peritonite infecciosa felina

    OpenAIRE

    Mota, Ana Luísa Deodato Ribeiro

    2011-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária A Peritonite Infecciosa Felina (PIF) é uma doença sistémica, mais frequente em animais jovens e gatos provenientes de gatis, provocada pelo Coronavírus Felino (FCoV), um vírus RNA cadeia simples sentido positivo com envelope. A proteína S viral permite a diferenciação entre os dois serótipos do FCoV. O vírus é transmitido por via fecal-oral, atinge os enterócitos, onde se multiplica. O modo peculiar de replicação viral f...

  2. Ciberespaço, vigilância e privacidade: o caso Google Street View

    Directory of Open Access Journals (Sweden)

    Elisianne Campos de Melo Soares

    2011-12-01

    Full Text Available

    O Google Street View é um serviço de mapeamento fotográfico de ruas criado pela multinacional Google em 2007. Em maio de 2010, Google admitiu que os veículos utilizados para a captura de imagens coletaram, inadvertidamente, dados pessoais (sobretudo mensagens de e-mail e vídeos transmitidos através de redes wi-fi privadas. Tendo em vista o problema de um possível atentado à vida privada e à segurança do tratamento de dados pessoais, pretende-se fazer uma breve análise do presente caso, trazendo à discussão algumas ideias tais como as de controle e vigilância no ciberespaço.

  3. 23-F: La noche de los transistores en la pantalla. La ficción audiovisual y la radio en el 23-F

    OpenAIRE

    Diego Pérez, Natalia

    2015-01-01

    Este proyecto se basa en los hechos ocurridos el 23 de febrero de 1981, cuando se produjo el asalto al Congreso de los Diputados y tuvo lugar el intento de Golpe de Estado. La radio fue el único medio de comunicación que pudo emitir lo que estaba pasando. Por otro lado, en los últimos años se han hecho series y una película sobre este acontecimiento, la ficción también puede ser información. En este trabajo se analizará la función de la radio aquel día y cómo la ficción ha transmitido ese pap...

  4. Sexo oral e HIV entre homens que fazem sexo com homens

    OpenAIRE

    Funari Sergio Luis

    2003-01-01

    A transmissão sexual do Vírus da Imunodeficiência Humana (HIV) representa um problema de saúde pública em todo o mundo, e homens que fazem sexo com homens encontram-se sob risco diferenciado para essa infecção. Ainda que existam evidências suficientes para se afirmar que o HIV pode ser transmitido via sexo oral, a percepção desse risco é ambígua, e relaciona-se de forma paradoxal com mudanças de atitude. Novos modelos de percepção de risco devem ser desenvolvidos em diversas áreas de conhecim...

  5. Préstamos del catalán en el léxico español

    OpenAIRE

    Prat Sabater, Marta

    2003-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada La investigación desarrollada en esta tesis doctoral, bajo el título de Préstamos del catalán en el léxico español, constituye un estudio diacrónico de los préstamos léxicos transmitidos entre dos lenguas románicas en una dirección concreta, en general, poco considerada: palabras del catalán que a lo largo de la historia han llegado a integrarse en el vocabulario español o en alguna de sus variedades dialectales (especia...

  6. Utilização de Bloquinhos de Montagem LEGO® para o Ensino dos Conceitos do Sistema Toyota de Produção

    Directory of Open Access Journals (Sweden)

    Alexandre Pinho

    2005-07-01

    Full Text Available Este artigo mostra como é possível facilitar  o aprendizado dos conceitos sobre o Sistema Toyota de Produção. É utilizada uma dinâmica de ensino através de bloquinhos de montagem LEGO® batizada de MIB – Montagem Interativa de Bloquinhos. Esta dinâmica além de abordar os principais conceitos sobre o Sistema de Toyota de Produção, também utiliza os conceitos sobre Just-in-time (JIT, tempo de setup, metodologia  5S e nivelamento de produção (Heijunka. O artigo descreve as quatro rodadas utilizadas na dinâmica MIB e como os conceitos descritos anteriormente são transmitidos aos alunos.

  7. Industrias culturales y de la comunicación: Mito y lógica de la creatividad y del empresario innovador

    Directory of Open Access Journals (Sweden)

    R de Mateo Pérez

    2015-12-01

    Full Text Available Introducción – Los discursos de los políticos, de los poderes económicos, de instituciones diversas como la Universidad, e incluso del lenguaje publicitario, y transmitidos por los medios de comunicación, se hacen eco del mantra del emprendedor y la innovación como fórmulas sagradas que, repetidas hasta la saciedad, hagan desaparecer la crisis del sistema económico, político y social en la que está inmerso el mundo globalizado. En este artículo se esclarecen el mito y la lógica de esos conceptos con análisis de caso en las industrias culturales y de comunicación.

  8. La "Comoedia Phylonis". Presentación, texto y traducción

    Directory of Open Access Journals (Sweden)

    Antonio Arbea

    2005-12-01

    Full Text Available La "Comoedia Phylonis" ("La comedia de Filón" es una de las piezas más desatendidas por los estudiosos del humanismo latino. Hasta hoy, en efecto, solo se la puede encontrar en el único manuscrito renacentista que la ha transmitido. Ofrecemos aquí la primera edición y la primera traducción de esta comedia a una lengua moderna, acompañadas de una breve presentación orientadora. ("Comoedia Phylonis" (“Philon’s Comedy” has been one of the most neglected pieces by Humanistic Latin Scholars. In fact, to this day it can only be found in the sole manuscript which has transmitted it.This article presents the first edition and the first translation of this comedy into a modern language, with a brief presentation.

  9. Multimedia Educativa para apoyar el Proceso de Enseñanza-Aprendizaje en la asignatura Comunicación Organizacional

    Directory of Open Access Journals (Sweden)

    Ordanis Nerza González Agüero

    2008-01-01

    Full Text Available Aborda lo referido al diseño de una multimedia educativa y sus indicaciones metodológicas, para favorecer el proceso de enseñanza - aprendizaje de la asignatura Comunicación Organizacional de la Carrera Comunicación Social, la que integra los contenidos de la asignatura y los presenta de una forma amena valiéndose de diversos recursos multimediales, lo que le facilita a los estudiantes la aprensión de los conocimientos transmitidos. Su concepción ha sido sustentada por el Proceso Unificado de Racional para el desarrollo de software (RUP y el empleo del sistema de autor multimedia builder, así como otras aplicaciones auxiliares.

  10. Cytoadherence capabilities of Plasmodium berghei ANKA and NK65 infected red blood cells in different malaria models

    OpenAIRE

    Albuquerque, Maria Inês Sousa de, 1988

    2011-01-01

    A malária é uma doença infecciosa responsável por mais de 225 milhões de infecções e 781 000 mortes por ano, a grande maioria de crianças até aos 5 anos de idade [1]. O seu impacto faz-se sentir não só a nível social, mas também a nível económico, sendo umas das causas da perpetuação do ciclo de pobreza que é vivido nos países afectados por esta doença [2]. A malária é causada por um parasita eucariota intracelular, do género Plasmodium, transmitido através da picada de um mosquito do género ...

  11. Atitudes de estudantes universitários frente ao consumo de materiais pornográficos University students' attitudes towards pornographic material consumption

    OpenAIRE

    Valeschka Martins Guerra; Fernando Cezar B. de Andrade; Mardonio Rique Dias

    2004-01-01

    A expansão do mercado de produtos pornográficos traz à tona a discussão acerca da influência dos valores transmitidos pela mídia no comportamento dos indivíduos. A questão da pornografia permanece sem o devido aprofundamento, pois o Brasil ainda é carente de pesquisas nesta área. Este estudo descreve a elaboração e validação de um instrumento de medida das atitudes frente ao consumo de materiais pornográficos, aplicada a 336 universitários e os dados submetidos à análise fatorial. Os 33 itens...

  12. A cultura organizacional: o impacto visível de uma dimensão invisível

    Directory of Open Access Journals (Sweden)

    Santos, Joana Vieira

    2010-01-01

    Full Text Available No quotidiano, muitas vezes sem que se apercebam, os sujeitos são influenciados pelas características culturais do meio em que vivem. A cultura é considerada toda a totalidade dos padrões de comportamento socialmente transmitidos, artes, crenças e muitos outros processos considerados a expressão de um determinado período, classe, comunidade ou população (Choudhry, Fang e Mohamed, 2007. Ao nível organizacional encontramos um conjunto de valores, normas e padrões de comportamento que influenciam os diversos membros. Este leque de valores varia consoante a organização, correspondendo à Cultura Organizacional. O presente artigo teórico constitui uma singela análise em torno da temática, com vista a despertar reflexão e, eventualmente, futuros estudos

  13. La educación pública en el legado de Condorcet

    Directory of Open Access Journals (Sweden)

    María Ángeles Abellán López

    2014-05-01

    Full Text Available Los conceptos que utilizamos sobre la educación como servicio público, universal, obligatorio, gratuito, laico, y libre fueron desarrollados durante la Revolución Francesa y su impacto en Europa se produjo a través de los ideales de libertad e igualdad. Condorcet representa el enlace entre la Ilustración y la Revolución francesa y nos ha transmitido un ideario político pedagógico que perdura en la actualidad. Los principios de la UNESCO, de la UNICEF, la Declaración de la Sorbona (1998, la Declaración de Bolonia (1999, la política educativa de la Unión Europea y los textos constitucionales, contienen muchas de las ideas que hace más de dos siglos defendió Condorcet.

  14. Epidemiologia molecular de surto de raiva bovina na região central do Rio Grande do Sul, 2012

    OpenAIRE

    Fábio Adriano Kanitz; Ananda Paula Kowalski; Helena Beatriz de Carvalho Ruthner Batista; Pedro Carnieli Junior; Rafael de Novaes Oliveira; Rudi Weiblen; Eduardo Furtado Flores

    2014-01-01

    A raiva é uma doença infecciosa do sistema nervoso central de mamíferos causada pelo vírus da raiva (RabV), geralmente, transmitido pela mordedura de animais infectados. No Brasil, os morcegos hematófagos Desmodus rotundus são as principais fontes de infecção do RabV para bovinos e equinos. Este artigo descreve uma investigação epidemiológica e molecular de surtos de raiva ocorridos na região central do Rio Grande do Sul, entre maio e agosto de 2012. Nesse período, 45 casos suspeitos de raiva...

  15. Estudios sobre la transmisión por "moscas blancas" (Homoptera: aleyrodidae de virus asociados con el "cuero de sapo" en yuca (Manihot esculenta Crantz

    Directory of Open Access Journals (Sweden)

    Angel S. Juan C.

    1987-12-01

    Full Text Available Estudios realizados en la zona endémica al "cuero de sapo" (Quilcacé, Cauca, encaminados a determinar la presencia de vectores de la enfermedad mostraron la existencia de dos virus asociados con "moscas blancas". El primero denominado "agente mosaico" fue transmitido por Bemisia tuberculata, el segundo asintomático, por Aleurotrachelus socialis. El 3.3% de la población de B. tuberculata utilizada transmitió el "agente mosaico" al clón M Col 2063 (Secundina y no a M Col 113: el 2.4 % de A. socialis transmitió el asintomático al clón Secundina y el 4.7% a M Col 113. El "agente mosaico", no fue identificado, pero si se demostró que el asintomático presente era CsXV; este sería el primer registro de un potexvirus transmitido por "moscas blancas". El papel de los dos virus en la etiología del “cuero de sapo” continúa en estudio.Whiteflies collected from a frogskin infested field in Quilcacé (Cauca were caged individually on M Col 2063 (Secundina and M Col 113 plants. It was possible to identify the species (Aleurotrachelus socialis, Trialeurodes variabilis and Bemisia tuberculata from pupae present on over 50% of the plants. A. socialis was found most f frequently and B. tuberculata the least com mom of the species. A. socialis was associated with the transmission of both CsXV and a serologically related strain, identified on the basis of symptoms produced on Nicotiana bentamiana, B. tuberculata was associated with the transmission of a mosaic agent to Secundina. The identify of the mosaic agent is unknown.

  16. Expression, purification and crystallization of the Crimean–Congo haemorrhagic fever virus nucleocapsid protein

    International Nuclear Information System (INIS)

    The Crimean–Congo haemorrhagic fever virus nucleocapsid protein was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 2.1 Å resolution. Crimean–Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing

  17. Thrips transmission of tospoviruses.

    Science.gov (United States)

    Rotenberg, Dorith; Jacobson, Alana L; Schneweis, Derek J; Whitfield, Anna E

    2015-12-01

    One hundred years ago, the disease tomato spotted wilt was first described in Australia. Since that time, knowledge of this disease caused by Tomato spotted wilt virus (TSWV) and transmitted by thrips (insects in the order Thysanoptera) has revealed a complex relationship between the virus, vector, plant host, and environment. Numerous tospoviruses and thrips vectors have been described, revealing diversity in plant host range and geographical distributions. Advances in characterization of the tripartite interaction between the virus, vector, and plant host have provided insight into molecular and ecological relationships. Comparison to animal-infecting viruses in the family Bunyaviridae has enabled the identification of commonalities between tospoviruses and other bunyaviruses in transmission by arthropod vectors and molecular interactions with hosts. This review provides a special emphasis on TSWV and Frankliniella occidentalis, the model tospovirus-thrips pathosystem. However, other virus-vector combinations are also of importance and where possible, comparisons are made between different viruses and thrips vectors. PMID:26340723

  18. Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System

    Institute of Scientific and Technical Information of China (English)

    Zhao-rui Zhou; Man-li Wang; Fei Deng; Tian-xian Li; Zhi-hong Hu; Hua-fin Wang

    2011-01-01

    Crimean-Congo Haemorrhagic Fever Virus(CCHFV)is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes,high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research,the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope,Virus-Like Particles (VLPs)with various size and morphology were observed in cytoplasmic vesicles in the infected cells.Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy(IEM).

  19. Possible involvement of eEF1A in Tomato spotted wilt virus RNA synthesis.

    Science.gov (United States)

    Komoda, Keisuke; Ishibashi, Kazuhiro; Kawamura-Nagaya, Kazue; Ishikawa, Masayuki

    2014-11-01

    Tomato spotted wilt virus (TSWV) is a negative-strand RNA virus in the family Bunyaviridae and propagates in both insects and plants. Although TSWV can infect a wide range of plant species, host factors involved in viral RNA synthesis of TSWV in plants have not been characterized. In this report, we demonstrate that the cell-free extract derived from one of the host plants can activate mRNA transcriptional activity of TSWV. Based on activity-guided fractionation of the cell-free extract, we identified eukaryotic elongation factor (eEF) 1A as a possible host factor facilitating TSWV transcription and replication. The RNA synthesis-supporting activity decreased in the presence of an eEF1A inhibitor, suggesting that eEF1A plays an important role in RNA synthesis of TSWV. PMID:25151062

  20. Complete Genome Sequencing of Four Geographically Diverse Strains of Batai Virus

    Science.gov (United States)

    Groseth, Allison; Matsuno, Keita; Dahlstrom, Eric; Anzick, Sarah L.; Porcella, Stephen F.

    2012-01-01

    Batai virus (BATV) is a widely distributed but poorly studied member of the Orthobunyavirus genus in the family Bunyaviridae and is of particular interest as a known participant in natural reassortment events. Both research and surveillance efforts on this and other related viruses have been hampered by the lack of available full-length sequence data covering all three genomic segments. Here, we report the complete genome sequence of four BATV strains (MM2222, Chittoor/IG-20217, UgMP-6830, and MS50) isolated from various geographical locations. Based on these data, we have determined that strain MS50 is in fact unrelated to BATV and likely represents as a novel genotype in the genus Orthobunyavirus. PMID:23166251

  1. Geographic distribution of Bhanja virus.

    Science.gov (United States)

    Hubálek, Z

    1987-01-01

    A review on the geographic distribution, vectors and hosts of Bhanja virus (Bunyaviridae) is based on reports about: isolations of the virus; antibody surveys. Bhanja virus has been isolated in 15 countries of Asia, Africa and Europe, and antibodies against it have been detected in 15 additional countries. Vector range includes ticks of the family Ixodidae (subfam. Amblyomminae; not subfam. Ixodinae): 13 species of 6 genera (Haemaphysalis, Dermacentor, Hyalomma, Amblyomma, Rhipicephalus and Boophilus) yielded the virus. Bhanja virus has only rarely been isolated from vertebrates (Atelerix, Xerus, Ovis, Bos; possibly bats), though antibodies have been detected frequently in a wide range of mammals (Ruminantia being the major hosts), in several species of birds (Passeriformes, Galliformes) and even reptiles (Ophisaurus apodus). Natural foci of the Bhanja virus infections are of the boskematic type (sensu Rosický), associated closely with pastures of domestic ruminants infested by ticks in the regions of tropical, subtropical and partly temperate climatic zones. PMID:3108117

  2. Crimean-Congo Hemorrhagic Fever.

    Science.gov (United States)

    Shayan, Sara; Bokaean, Mohammad; Shahrivar, Mona Ranjvar; Chinikar, Sadegh

    2015-01-01

    Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the Bunyaviridae family and Nairovirus genus. The viral genome consists of 3 RNA segments of 12 kb (L), 6.8 kb (M), and 3 kb (S). Crimean-Congo hemorrhagic fever (CCHF) is the most widespread tickborne viral infection worldwide: it has been reported in many regions of Africa, the Middle East, and Asia. The geographical distribution of CCHFV corresponds most closely with the distribution of members of the tick genera, and Hyalomma ticks are the principal source of human infection. In contrast to human infection, CCHFV infection is asymptomatic in all species. Treatment options for CCHF are limited; immunotherapy and ribavirin are effective in the treatment of CCHF; the efficacy of ribavirin in the treatment of CCHF has not yet been proven. This article reviews the history, epidemiology, clinical symptoms, pathogenesis, diagnosis, and treatment of CCHFV, as well as the development of a vaccine against it. PMID:26199256

  3. Crimean-Congo hemorrhagic fever virus nucleoprotein suppresses IFN-beta-promoter-mediated gene expression.

    Science.gov (United States)

    Fajs, Luka; Resman, Katarina; Avšič-Županc, Tatjana

    2014-02-01

    Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the family Bunyaviridae and is a causative agent of severe hemorrhagic disease. Knowledge regarding the pathogenesis of CCHFV is limited due to the requirement for high-containment laboratories and the lack of an immunocompetent animal host. Previous studies have shown that CCHFV delays the activation of the human innate immune response, specifically, the type I interferon response. Our study results show that antagonism of the interferon-beta promoter is mediated by the nucleoprotein of CCHFV strain Hoti, while strains IbAr10200 and AP92 do not suppress the activity of the IFN-beta promoter. Our results also suggest that several viral factors may provide antagonistic action against the type I interferon response. PMID:23990053

  4. Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran

    Directory of Open Access Journals (Sweden)

    Sadegh Chinikar

    2015-10-01

    Full Text Available  Background: Crimean-Congo Hemorrhagic Fever Virus (CCHFV belongs to genus Nairovirus and family Bunyaviridae. The main aim of this study was to investigate the extent of recombination in S-segment genome of CCHFV in Iran.Methods: Samples were isolated from Iranian patients and those available in GenBank, and analyzed by phyloge­netic and bootscan methods.Results: Through comparison of the phylogenetic trees based on full length sequences and partial fragments in the S-segment genome of CCHFV, genetic switch was evident, due to recombination event. Moreover, evidence of multi­ple recombination events was detected in query isolates when bootscan analysis was used by SimPlot software.Conclusion: Switch of different genomic regions between different strains by recombination could contribute to CCHFV diversification and evolution. The occurrence of recombination in CCHFV has a critical impact on epidemi­ological investigations and vaccine design. 

  5. Molecular epidemiology of Crimean- Congo hemorrhagic fever virus genome isolated from ticks of Hamadan province of Iran

    DEFF Research Database (Denmark)

    Tahmasebi, F; Ghiasi, Seyed Mojtaba; Mostafavi, E;

    2010-01-01

    BACKGROUND & OBJECTIVES: Crimean-Congo hemorrhagic fever (CCHF) virus is a tick-borne member of the genus Nairovirus, family Bunyaviridae. CCHFV has been isolated from at least 31 different tick species. The virus is transmitted through the bite of an infected tick, or by direct contact with CCHFV......-infected patients or the products of infected livestock. This study was undertaken to study the genetic relationship and distribution of CCHFV in the tick population of Hamadan province of Iran. METHOD: In this study, RT-PCR has been used for detection of the CCHFV genome. RESULTS: This genome was detected in 19.......2% of the ticks collected from livestock of different regions of the Hamadan province in western Iran. The infected species belonged to Hyalomma detritum, H. anatolicum, Rhipicephalus sanguineus and Argas reflexus. With one exception, genetic analysis of the virus genome isolates showed high sequence...

  6. An update on crimean congo hemorrhagic fever

    Directory of Open Access Journals (Sweden)

    Suma B Appannanavar

    2011-01-01

    Full Text Available Crimean Congo hemorrhagic fever (CCHF is one of the deadly hemorrhagic fevers that are endemic in Africa, Asia, Eastern Europe, and the Middle East. It is a tick-borne zoonotic viral disease caused by CCHF virus of genus Nairovirus (family Bunyaviridae. CCHF not only forms an important public health threat but has a significant effect on the healthcare personnel, especially in resource-poor countries. India was always a potentially endemic area until an outbreak hit parts of Gujarat, taking four lives including the treating medical team. The current review is an attempt to summarize the updated knowledge on the disease particularly in modern era, with special emphasis on nosocomial infections. The knowledge about the disease may help answer certain questions regarding entry of virus in India and future threat to community.

  7. Schmallenberg virus infection of ruminants: challenges and opportunities for veterinarians

    Directory of Open Access Journals (Sweden)

    Claine F

    2015-06-01

    Full Text Available François Claine, Damien Coupeau, Laetitia Wiggers, Benoît Muylkens, Nathalie Kirschvink Veterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS, University of Namur (UNamur, Namur, Belgium Abstract: In 2011, European ruminant flocks were infected by Schmallenberg virus (SBV leading to transient disease in adult cattle but abortions and congenital deformities in calves, lambs, and goat kids. SBV belonging to the Simbu serogroup (family Bunyaviridae and genus Orthobunyavirus was first discovered in the same region where bluetongue virus serotype 8 (BTV-8 emerged 5 years before. Both viruses are transmitted by biting midges (Culicoides spp. and share several similarities. This paper describes the current knowledge of temporal and geographical spread, molecular virology, transmission and susceptible species, clinical signs, diagnosis, prevention and control, impact on ruminant health, and productivity of SBV infection in Europe, and compares SBV infection with BTV-8 infection in ruminants. Keywords: Schmallenberg virus, Europe, ruminants, review

  8. Hantaviral Proteins: Structure, Functions, and Role in Hantavirus Infection

    Science.gov (United States)

    Muyangwa, Musalwa; Martynova, Ekaterina V.; Khaiboullina, Svetlana F.; Morzunov, Sergey P.; Rizvanov, Albert A.

    2015-01-01

    Hantaviruses are the members of the family Bunyaviridae that are naturally maintained in the populations of small mammals, mostly rodents. Most of these viruses can easily infect humans through contact with aerosols or dust generated by contaminated animal waste products. Depending on the particular Hantavirus involved, human infection could result in either hemorrhagic fever with renal syndrome or in Hantavirus cardiopulmonary syndrome. In the past few years, clinical cases of the Hantavirus caused diseases have been on the rise. Understanding structure of the Hantavirus genome and the functions of the key viral proteins are critical for the therapeutic agents’ research. This paper gives a brief overview of the current knowledge on the structure and properties of the Hantavirus nucleoprotein and the glycoproteins. PMID:26640463

  9. Antibody levels to hantavirus in inhabitants of western Santa Catarina State, Brazil

    Directory of Open Access Journals (Sweden)

    William Marciel de Souza

    2012-08-01

    Full Text Available Hantavirus cardiopulmonary syndrome (HCPS is an infectious disease caused by hantaviruses of the family Bunyaviridae, and is transmitted by aerosols of excreta of infected rodents. The aim of the present study was to determine antibody levels to hantavirus in the population that lives at frontier of Brazil and Argentina. Participated of the study 405 individuals living in the municipalities of Bandeirante, Santa Helena, Princesa and Tunapolis, state of Santa Catarina, Brazil. IgG antibodies to hantavirus were analyzed in sera by an ELISA that uses a recombinant N protein of Araraquara hantavirus as antigen. The results were also confirmed by immunofluorescent test. Eight individuals showed antibodies to hantavirus (1.97% positivity, with serum titers ranging from 100 to 800. Six seropositives were males, older than 30 years and farmers. Our results reinforce previous data on hantavirus circulation and human infections in the southern border of Brazil with Argentina.

  10. Detection of Hantaan virus RNA from anti-Hantaan virus IgG seronegative rodents in an area of high endemicity in Republic of Korea.

    Science.gov (United States)

    No, Jin Sun; Kim, Won-Keun; Kim, Jeong-Ah; Lee, Seung-Ho; Lee, Sook-Young; Kim, Ji Hye; Kho, Jeong Hoon; Lee, Daesang; Song, Dong Hyun; Gu, Se Hun; Jeong, Seong Tae; Kim, Heung-Chul; Klein, Terry A; Song, Jin-Won

    2016-04-01

    Hantaan virus (HTNV), of the family Bunyaviridae, causes hemorrhagic fever with renal syndrome (HFRS) in humans. Although the majority of epidemiologic studies have found that rodents are seropositive for hantavirus-specific immunoglobulin, the discovery of hantavirus RNA in seronegative hosts has led to an investigation of the presence of HTNV RNA in rodents captured in HFRS endemic areas. HTNV RNA was detected in seven (3.8%) of 186 anti-HTNV IgG seronegative rodents in Republic of Korea (ROK) during 2013-2014. RT-qPCR for HTNV RNA revealed dynamic virus-host interactions of HTNV in areas of high endemicity, providing important insights into the epidemiology of hantaviruses. PMID:26917012

  11. Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia

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    Nikolić Valentina

    2014-01-01

    Full Text Available The Dobrava-Belgrade virus (DOBV is a member of the Bunyaviridae family, genus Hantavirus, possessing a single-stranded RNA genome consisting of three segments, designated L (large, M (medium and S (small. In this study, we present phylogenetic analysis of a newly detected DOBV strain isolated from Apodemus agrarius. Analysis was based on partial L and S segment sequences, in comparison to previously published DOBV sequences from Serbia and elsewhere. A phylogenetic tree based on partial S segment revealed local geographical clustering of DOBV sequences from Serbia, unrelated to host (rodent or human. The topology of the phylogenetic tree was confirmed with a high percent of completely or partially resolved quartets in likelihood-mapping analysis, whereas no evidence of possible recombination in the examined S segment data set was found.

  12. Hantaviruses as emergent zoonoses

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    LS Ullmann

    2008-01-01

    Full Text Available Hantaviruses belong to the Bunyaviridae family, which consists of vector-borne viruses. These viruses can provoke two infection types: hemorrhagic fever with renal syndrome (HFRS - which occurs in the Old World - and hantavirus cardiopulmonary syndrome (HCPS - an emergent zoonosis that can be found in many countries of the western hemisphere. Rodents are hantavirus reservoirs and each species seems to host a different virus type. Humans acquire the infection by inhaling contaminated aerosol particles eliminated by infected animals. The factors involved in the emergence of hantavirus infections in the human population include ecological modifications and changes in human activities. The most important risk factor is contact between man and rodents, as a result of agricultural, forestry or military activities. Rodent control remains the primary strategy for preventing hantavirus diseases, including via health education and hygienic habits.

  13. Perspectives of antiviral RNA interference (RNAi pathway of insects with special reference to mosquito in the context of dengue infection: a review

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    Probal Basu

    2014-09-01

    Full Text Available RNA interference is a post-transcriptional sequence selective gene control mechanism. Antiviral RNA interference (RNAi pathway is one of the most momentous constituents of the insect innate immune system that can stymie versatile range of RNA virus like flavivirus. It has been demonstrated that RNA production by alphavirus replication is higher in proportion compared to flavivirus replication in mosquito cells. Studies demonstrated that infection by virus from Togaviridae and Bunyaviridae family of arbovirus to mosquito cells causes defect in RNAi response in-vitro but interestingly, it has also been stated that Dengue virus (DENV could be actively inhibited by RNA interference (RNAi. This article is an endeavor to review the perspectives of the functional significance of antiviral RNA interference as a potent agent of controlling dengue infection in the vector.

  14. Molecular detection of Eastern Equine Encephalitis virus in mosquitoes from La Pintada (Antioquia

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    Richard Hoyos L

    2015-09-01

    Full Text Available Objective. The detection of emerging and re-emerging arboviruses in mosquitoes from urban and rural areas, is fundamental for predict possible epidemic outbreaks in human populations. The Municipality of La Pintada (Antioquia, is characterized by the presence of dry tropical forest relicts, fishing, tourism, farms and mining. An entomological research was performed for explore the possible circulation of arboviruses of public health importance. Materials and methods. Mosquitoes were captured in urban and rural sites in February-April of 2012. The specimens were stored in liquid nitrogens tanks and were grouped using taxonomic keys for genera. RNA extraction from pools and generic/nested RT-PCR was performed for Flavivirus, Alphavirus, Orthobunyavirus (Group Bunyamwera and Phlebovirus. Results. 1274 mosquitoes were collected, mainly belonging to Culex and Aedes genera. RNA extracts of 64 pools were tested by RT-PCR and one pool was positive for Alphavirus. Sequencing of the RT-PCR product and the analysis with sequences storage in GenBank designate the presence of Eastern equine encephalitis virus (EEEV. Conclusions. This is the first record of natural infection from EEEV in mosquitoes from La Pintada (Antioquia, an area with ecological elements that favor the emergence of emerging and re-emerging arboviruses of medical and veterinarian importance.

  15. Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern.

    Science.gov (United States)

    Maroli, M; Feliciangeli, M D; Bichaud, L; Charrel, R N; Gradoni, L

    2013-06-01

    Phlebotomine sandflies transmit pathogens that affect humans and animals worldwide. We review the roles of phlebotomines in the spreading of leishmaniases, sandfly fever, summer meningitis, vesicular stomatitis, Chandipura virus encephalitis and Carrión's disease. Among over 800 species of sandfly recorded, 98 are proven or suspected vectors of human leishmaniases; these include 42 Phlebotomus species in the Old World and 56 Lutzomyia species in the New World (all: Diptera: Psychodidae). Based on incrimination criteria, we provide an updated list of proven or suspected vector species by endemic country where data are available. Increases in sandfly diffusion and density resulting from increases in breeding sites and blood sources, and the interruption of vector control activities contribute to the spreading of leishmaniasis in the settings of human migration, deforestation, urbanization and conflict. In addition, climatic changes can be expected to affect the density and dispersion of sandflies. Phlebovirus infections and diseases are present in large areas of the Old World, especially in the Mediterranean subregion, in which virus diversity has proven to be higher than initially suspected. Vesiculovirus diseases are important to livestock and humans in the southeastern U.S.A. and Latin America, and represent emerging human threats in parts of India. Carrión's disease, formerly restricted to regions of elevated altitude in Peru, Ecuador and Colombia, has shown recent expansion to non-endemic areas of the Amazon basin. PMID:22924419

  16. Search for tick-borne pathogens in the Svalbard Archipelago and Jan Mayen

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    Jana Elsterová

    2015-10-01

    Full Text Available The tick species Ixodes uriae, parasitizing seabirds in the Arctic, may transmit many pathogens including various arboviruses, Borrelia spirochetes and Babesia apicomplexans. These pathogens may pose an important additional stress to seabirds, which are already stressed by environmental changes such as pollutants and decreased food availability. Here, we present the results of the first screening for arboviruses of the genera Flavivirus, Alphavirus, Orthobunyavirus, Phlebovirus and Orbivirus, as well as Borrelia spirochetes and Babesia apicomplexans from Svalbard and Jan Mayen. Using polymerase chain reaction technology with genus-specific primers, we tested 89 ticks collected on Jan Mayen, Bjørnøya and Spitsbergen between 2008 and 2012. We did not detect any of the screened tick-borne pathogens. Nevertheless, these pathogens may be introduced to Svalbard and Jan Mayen by migratory birds in the near future. The increasing numbers of ticks appearing in the studied areas make this introduction even more likely. Such an introduction would have serious impact on seabird ecology as well as on human public health. Therefore, continuous careful surveillance and monitoring of possible tick-borne pathogen introductions is important.

  17. Savoirs professionnels et prévention des TMS : portrait de leur transmission durant la formation et perspectives d’intervention Professional knowledge and MSD prevention: portrait of their transmission during training and the intervention perspective Saberes profesionales y prevención de las LMS : un retrato de su transmisión durante la formación y perspectivas de intervención

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    Sylvie Ouellet

    2009-11-01

    demonstrated. Intervention scenarios are proposed.Durante la organización de formaciones en empresas donde el trabajo es considerado manual y repetitivo, el formador es generalmente elegido entre los trabajadores experimentados que poseen un saber-hacer reconocido. Este estudio cuyo objetivo es de prevenir las lesiones músculo esqueléticas, analiza los tipos de saberes transmitidos a los aprendices por los trabajadores-formadores en una empresa del sector agroalimenticio. El análisis de los sabers transmitidos verbalmente por los formadores fue efectuada a partir de grabaciones audio provenientes del seguimiento cotidiano de las formaciones. El proceso ergonómico que se desarrolló en este estudio permitió mostrar que los saberes relacionados con la protección de la salud, el porqué de los gestos y los puntos de referencia para el trabajo son más difícilmente transmitidos que los otros saberes. Se puso en evidencia que el fenómeno de transmisión es complejo y que los formadores necesitan ser acompañados en el desarrollo de competencias para transmitir los saberes. Proponemos perspectivas de intervención.

  18. Professional knowledge and MSD prevention: portrait of their transmission during training and the intervention perspective Savoirs professionnels et prévention des TMS : portrait de leur transmission durant la formation et perspectives d’intervention Saberes profesionales y prevención de las LMS : un retrato de su transmisión durante la formación y perspectivas de intervención

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    Sylvie Ouellet

    2009-11-01

    perspectives d’intervention sont proposées.Durante la organización de formaciones en empresas donde el trabajo es considerado manual y repetitivo, el formador es generalmente elegido entre los trabajadores experimentados que poseen un saber-hacer reconocido. Este estudio cuyo objetivo es de prevenir las lesiones músculo esqueléticas, analiza los tipos de saberes transmitidos a los aprendices por los trabajadores-formadores en una empresa del sector agroalimenticio. El análisis de los sabers transmitidos verbalmente por los formadores fue efectuada a partir de grabaciones audio provenientes del seguimiento cotidiano de las formaciones. El proceso ergonómico que se desarrolló en este estudio permitió mostrar que los saberes relacionados con la protección de la salud, el porqué de los gestos y los puntos de referencia para el trabajo son más difícilmente transmitidos que los otros saberes. Se puso en evidencia que el fenómeno de transmisión es complejo y que los formadores necesitan ser acompañados en el desarrollo de competencias para transmitir los saberes . Proponemos perspectivas de intervención.

  19. Campañas de prevención del cáncer en el contexto de la Publicidad de Servicio Público: un análisis de los mensajes y de los recursos creativos.Campanhas de prevenção do cancro no contexto da Publicidade de Serviço Púbico: uma análise das mensagens e dos recursos criativos.Campaigns for cancer prevention in the context of Public Service Advertising: an analysis of messages and creative resources

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    Maria van Schoor

    2011-01-01

    Full Text Available The present study aims to understand the structure of preventive campaigns against cancer focusing on the arguments that are used and the way they are transmitted. The research work comprised a content analysis of 72 advertising films produced in various continents representing, in general, the different approaches used nowadays in this field. We can point out, as main conclusions, that the cancer prevention campaigns use a considerable percentage of positive approaches, do not use fear appeals or display the shocking consequences as a result of negligent behaviour. The main messages emphasize prevention through creative formats that intend to establish an empathy and proximity with the target audience.Con este trabajo pretendemos comprender la estructura de las campañas de prevención del cáncer a nivel internacional, incidiendo en el análisis de los argumentos utilizados y la forma en la que estos son transmitidos. Realizamos un análisis de contenido de 72 anuncios publicitarios audiovisuales procedentes de diversos continentes y que ilustran, en gran parte, los diferentes abordajes utilizados actualmente en este campo. Fue posible constatar que las campañas de prevención del cáncer utilizan un porcentaje considerable de anuncios desde una perspectiva positiva, en los cuales no se utiliza el miedo ni consecuencias impactantes como resultado de los comportamientos de riesgo. En su mayoría, los anuncios utilizan un abordaje positivo, desde una óptica preventiva, a través de formatos creativos que procuran una gran empatía y proximidad con los destinatarios del mensaje.Este estudo tem como objectivo comprender a estrutura das campanhas de prevenção contra o cancro, a nível internacional, incidindo a sua análise sobre os argumentos utilizados e sobre a forma como são transmitidos. Na componente empírica do trabalho, realizou-se uma análise de conteúdo de 72 filmes publicitários provenientes de diversos continentes e que

  20. Posthumanist’s values in worldwide movies

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    Claudio Pensieri

    2014-01-01

    Full Text Available La Pop-Filosofía puede transmitir muchos mensajes importantes, debido a su forma popular de comunicarse con la gente. Podemos decir que uno de los medios más importantes que se utilizan en la pop-filosofía para alcanzar sus objetivos es el cine. De hecho en 2011 se produjeron 818 películas. En USA los ingresos del cine en el 2012 fueron 1.360 millones, mientras que en Europa fueron 1.190 millones. Por esta razón hemos querido experimentar una nueva metodología para estudiar la “percepción” de valores transmitidos por el cine entre los estudiantes de la Universidad Campus Bio-Médico. En la primera fase, hemos pedido a 296 estudiantes cual fueron los títulos de las películas que han visto, que tratan sobre la divulgación de los valores posthumanistas. A partir de esta primera fase identificamos las 5 películas más populares y citadas. En la segunda fase entrevistamos 175 estudiantes sobre cual idea de Dios, Hombre y Naturaleza, en su opinión, transmitían las 5 películas. Nuestro estudio indica que existe una real diferencia entre el contenido teórico posthumanista de las mas importantes películas y lo que el estudiante de nuestra muestra percibe.

  1. Fuentes hagiográficas referentes a Prudencio de Armentia, obispo de Tarazona

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    Igartua Ugarte, Nora

    2004-06-01

    Full Text Available The author analyzes the controversial hagiographical sources relative to Prudencio of Armentia, bishop of Tarazona. They're several documents of literary character that narrate Prudencio's life, although certain textual variants don't correspond the mentioned religious figure. In this study the author tries to answer some questions linked to the reliability of these sources: which is the hagiographical value of each of these testimonies in connection with the prelate of Tarazona, how old are they and which way have they been transmitted through the time.

    La autora analiza las controvertidas fuentes hagiográficas relativas a Prudencio de Armentia, obispo de Tarazona. Son varios los documentos de carácter literario que narran la vida de Prudencio, aunque hay determinadas variantes textuales que no corresponden al citado personaje religioso. En el presente estudio se pretende responder a diversas cuestiones relacionadas con la fiabilidad de estas fuentes: cuál es el valor hagiográfico de cada uno de estos testimonios en relación con el prelado turiasonense, qué antigüedad poseen y cómo se han transmitido a lo largo del tiempo.

  2. LA RACIONALIDAD ECONÓMICA EN NUEVA PERSPECTIVA

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    Jorge Arturo Chaves

    2002-08-01

    Full Text Available Las amenazas a la vida en el planeta, los hechos que han “asaltado al dogma económico” (Daly y Cobb, 1993 tienen mucho que ver con una doble toma de conciencia en la sociedad contemporánea. La primera, sobre lo restringida y esterilizante que resulta una visión economicista para enfrentar los problemas que más preocupan al ser humano hoy: las heridas infligidas al ecosistema, el aumento de la pobreza y la desigualdad, y el crecimiento desproporcionado de la actividad humana en relación a las posibilidades de la biosfera.La segunda, implicada en la anterior, la de darse cuenta que esa limitación está asociada al concepto de suyo estrecho y parcial de racionalidad económica. Enfrentamos aquí el problema del reduccionismo transmitido en el discurso y en la práctica misma de la disciplina económica que, sin embargo, no es exclusivo de ésta ni es atribuible sólo a deficiencias internas de los profesionales que la practican. Existe toda una larga tradición dentro de la cual se ha ido reduciendo de manera progresiva el concepto de razón humana y se ha ido produciendo ese concepto estrecho de racionalidad del que ahora la humanidad realiza esfuerzos por librarse.

  3. Espinheira-santa (Maytenus ilicifolia Mart. ex Reiss.: saber de erveiros e feirantes em Pelotas (RS

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    C. ALMEIDA

    2015-01-01

    Full Text Available RESUMO O objetivo deste trabalho é descrever o conhecimento popular relacionado à espinheira-santa (Maytenus ilicifolia entre erveiros e feirantes que comercializam a planta no centro de Pelotas, Rio Grande do Sul, Brasil. Foi realizada uma pesquisa de abordagem qualitativa, exploratória e descritiva, de agosto a outubro de 2013. Entrevistas semi-estruturadas, com questões sobre os saberes e práticas relacionados à espinheira-santa, foram aplicadas a cinco erveiros e três feirantes do mercado informal de plantas medicinais. Para a análise dos dados foi utilizada a proposta operativa de Minayo, sendo Capra o referencial teórico para este estudo. Foi verificado que o saber relacionado ao uso da espinheira-santa é transmitido de geração a geração, embora existam outras fontes de conhecimento. As indicações do uso popular da espinheira-santa com finalidade terapêutica estão relacionadas a distúrbios gástricos, cicatrização e depuração do sangue. As diferentes formas dos sujeitos deste estudo interagirem com o ambiente, com sua família e em outras relações interpessoais resultam em diferentes saberes relacionados à espinheira-santa.

  4. Ventajas de la implementación de la práctica de gobierno corporativo en las empresas

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    Dilia Castillo

    2007-10-01

    Full Text Available El objetivo primordial de una empresa es maximizar la riqueza corporativa. Lapráctica de Gobierno Corporativo (Corporate Governance de una organización protege los intereses de sus diversos grupos. Ésta es tarea directiva de la sentidades públicas o privadas, para asegurar la aplicación de los principios empresariales como equidad, justicia, economía, verdad, honestidad, eficacia,y responsabilidad tanto para clientes externos como internos. De igual modo, garantiza la reproducción de una información fidedigna y transparente, orientada por la Dirección, la cual estará pendiente de revisar y guiar la estrategia de la compañías, sus planes de acción, las políticas de contratación, los objetivos de desempeño, incluyendo el servicio posventa al cliente y la planeación. Así mismo, está pendiente de que la información publicitaria y de promoción sean realmente las que se estén ejecutando y cumpliendo, para garantizar que todos los esfuerzos sean transmitidos al consumidor final como valor agregado que asegura su fidelidad a la empresa.

  5. Libros y lecturas para jóvenes. (La transmisión de valores a través de la literatura infantil y juvenil : el caso de la lij catalana 1939- 1985

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    Ramón BASSA i MARTÍN

    2010-03-01

    Full Text Available RESUMEN: La finalidad principal de este trabajo es analizar el mensaje educativo que durante casi medio siglo ha transmitido la literatura infantil y juvenil en lengua catalana, a partir de un análisis del contenido de una muestra representativa de libros, agrupando los campos léxico-semánticos de los textos en torno a diez grandes bloques (o sistemas ideológico-conceptuales, al tiempo que descubrir el modelo de intervención socioeducativa que esta literatura tuvo que construir históricamente en un determinado espacio social y cultural para sus jóvenes lectores. ABSTRACT: The primary aim of this paper is to analyse the educational message transmitted for almost half a century through children's literature in Catalan, based on an analysis of the content of a representative sample of books by grouping the texts' lexical-semantical fields around ten large blocks (or conceptual-ideological systems, while discovering the model of socioeducational intervention this literature historically had to construct within a determined social and cultural space for its young readers.

  6. Exposición humana a disruptores endocrinos

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    A. Rivas

    2004-01-01

    Full Text Available Numerosos estudios han asociado las patologías observadas en distintas especies animales y en el hombre con la exposición a contaminantes medio ambientales con actividad hormonal. Se ha acuñado el término de disruptores endocrinos (Endocrine Disrupting Chemicals / EDCs para definir el conjunto de compuestos químicos que interaccionan con el sistema endocrino, sobre el que inducen efectos potencialmente debidos a su capacidad para:1 mimetizar la acción de las hormonas endógenas; 2 antagonizar la acción de las hormonas; 3 alterar su patrón de síntesis y metabolismo; o bien 4 modular los niveles de los receptores correspondientes.La exposición humana a disruptores endocrinos es universal y puede provenir de numerosas fuentes. Además, los compuestos acumulados en la grasa son transmitidos a la descendencia a través de la madre durante la gestación y después de la lactancia.Los efectos sobre la salud humana de la exposicición continua a los disruptores endocrinos necesitan ser investigados en más profundidad.

  7. Transmissão de imunidade antiamarílica da mãe aos filhos, em camondongos

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    Herminio Linhares

    1943-01-01

    Full Text Available 1. Fêmeas imunizadas ativa e passivamente, podem transmitir anticorpos protetores a seus filhos. 2. Os camondongos adquirem imunidade pela placenta e principalmente após o nascimento, pos ingestão de leite de fêmea imune. 3. O virus não pode ser transmitido por qualquer das duas vias. 4. A imunidade transferida pela placenta desaparece muito mais rapidamente que a imunidade transmitida pelo leite. 5. Filhos de fêmea normal, rapidamente adquirem imunidade se forem amamentados em fêmea imune. 6. Camondongos com mais de 10 dias absorvem tão bem anticorpos como nos primeiros dias do nascimento, e o leite veicula durante todo o periodo de amamentação substâncias protetoras.1. Females rendered immune actively and passively against yellow fever virus may transmit protective antibodies to their young ones. 2. Mice acquire immunity through the placenta and principally after birth by ingestion of milk of an immune female. 3. The virus can not be transmitted by either of the two ways. 4. The immunity transferred through the placenta disappears much more rapidly than immunity transmited by milk. 5. Young ones from a normal female acquire immunity rapidly if fed by an immune female. 6. Mice of more than 10 days absorb antibodies just as well as during the first days of their birth, and the milk carries with it protective substances during the whole suckling period.

  8. Relación del Virus del Oeste del Nilo con las Aves Silvestres

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    Diego Soler-Tovar

    2009-06-01

    Full Text Available El Virus del Oeste del Nilo (VON es un Flavivirus que produce una encefalomielitis y puede afectar aves y mamíferos; los efectos varían desde poco notorios hasta la muerte, y en su difusión es importante la participación de especies animales como las aves donde es transmitido a través de mosquitos vectores. El VON se conoce de Uganda desde 1937 y está ampliamente distribuido en África, el oriente y la región sur y tropical de Eurasia. En el Hemisferio Occidental, el VON fue la primera causa de enfermedad en poblaciones de aves y humanos en Nueva York en 1999 y la epizootia ha continuado hasta ahora. La presencia del virus en Norteamérica junto con un número relativamente grande de aves migratorias susceptibles al agente, hacen de este virus de especial atención por su posible impacto sobre la fauna silvestre, los animales domésticos y el hombre en Sudamérica. La infección y mortalidad de aves normalmente precede a las epidemias en humanos y por lo tanto el monitoreo de aves es una herramienta preventiva efectiva que debe ser considerada seriamente.

  9. El juramento de fidelidad a Octaviano del año 32 a. C.

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    María Concepción Rosado Martín

    2010-03-01

    Full Text Available El juramento de fidelidad del año 32 a. C. situó a Octaviano al frente de un bloque, liderado por Italia y secundado por las provincias occidentales, preparado para enfrentarse a Marco Antonio, quien estaba secundado por Cleopatra y por los territoriosorientales. Suetonio, Dión Casio y el propio Augusto en las Res Gestae Divi Augusti nos han transmitido la existencia de este juramento. El texto original no se conserva por lo quese ha comparado con otros juramentos de fidelidad, tanto de época republicana como imperial. Nuestro objetivo es estudiar cómo se gestó este juramento, en qué consistía y analizar otros juramentos de fidelidad parecidos al del año 32 a. C. Con este juramento, Italia y Occidente quedaron unidos políticamente a Octaviano de cara al inminente enfrentamiento de éste contra Marco Antonio cuya consecuencia será el paso de la República al Principado.

  10. "La ambigüedad, una forma de evidencia" en el "Prólogo general de los cuentos de Canterbury" de Geoffrey Chaucer

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    Antonio PRIETO MARTÍNEZ

    2009-11-01

    Full Text Available La capacidad lírica de Chaucer en los Cuentos de Canterbury no se deja esperar. Los primeros versos del Prólogo General (Whan that April in hir corages, versos 1-11 son como una carta de presentación del poeta, que quiere dejar constancia de su modo de hacer, por una parte, y por otra, de su actitud de renovación con respecto a obras anteriores. Porque parece que Chaucer se hubiese sentado ante su mesa de trabajo con el mismo espíritu vivificante que Abril ha transmitido a Marzo, con la misma fuerza con la que Céfiro anima a la naturaleza, dispuesto a engendrar una obra nueva, repleta de vigor y de alegría. James Winny comparte esta idea cuando dice que «The natural forces which bring warmth and moisture to the earth, encouraging growth and fertility, stand in a direct relationship with the powerful impulse working upon the poet».

  11. Para além de Salvador e do Recôncavo baiano: o culto aos santos na América Portuguesa Beyond Salvador and the Recôncavo baiano: cult to the saints in Portuguese America

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    Tânia Maria Pinto de Santana

    2010-01-01

    Full Text Available Este texto pretende discutir alguns aspectos ligados à questão dos poucos avanços da catequese católica entre os negros, proposta pelo Clero na América - tomando como referência a cidade de Salvador e seu Recôncavo. Esses estariam ligados à sobrevivência de práticas pré-cristãs entre os africanos e seus descendentes. Marcados pela presença dos elementos cósmicos em suas concepções religiosas, esses priorizaram em suas crenças, a relação com os elementos da natureza, fazendo prevalecer uma experiência religiosa que colocava à margem conteúdos básicos transmitidos através da doutrina e dos ritos instituídos pela Igreja Católica.This text intends to discuss some aspects referent to the few advances of the Catholic catechism among the blacks, proposed by the Clergy in America - taking as reference the city of Salvador and its Recôncavo. These would be linked to the survival of pre-Christian practices among the Africans and their descendants. Marked by the presence of the cosmic elements in their religious concepts, these prioritized in their beliefs, the relation with the elements from the nature, prevailing a religious experience that laid aside basic contents transmitted through the doctrine and the rites established by the Catholic Church.

  12. Detecção, transmissão e patogenicidade de fungos em sementes de angico-vermelho (Parapiptadenia rigida

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    Caciara Gonzatto Maciel

    2012-12-01

    Full Text Available Angico-vermelho (Parapiptadenia rigida (Benth. Brenan é uma espécie nativa de grande valor ecológico e econômico, importante para a recomposição de áreas degradadas. O presente trabalho avaliou incidência, transmissão e patogenicidade de fungos associados a sementes de angico-vermelho de distintas procedências do Estado do Rio Grande do Sul. Para isso, utilizaram-se três amostras de sementes, com as quais realizaram-se testes de germinação, sanidade empregando-se o método do papel-filtro (PF e de plaqueamento em batata-dextrose-ágar (BDA, transmissão e patogenicidade dos fungos. A germinação das sementes de angico-vermelho variou de 63 a 91 %. Os fungos considerados potencialmente patogênicos encontrados associados as sementes de angico-vermelho foram: Alternaria sp.; Botrytis sp.; Fusarium sp.; Cladosporium sp. e Pestalotia sp.; sendo que Fusarium sp. foi detectado em todas as amostras pelo método PF, e foi transmitido via semente causando má formação do sistema radicular e dos cotilédones e tombamento de pré emergência. Sua patogenicidade foi confirmada.

  13. Transmissão do Trypanosoma cruzi em três gerações de Cavia porcellus sem a participação de triatomíneos

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    Ítalo A. Sherlock

    1976-02-01

    Full Text Available Foi verificada a transmissão sucessiva do T. cruzi em três gerações da cobaia Cavia porcellus sem a participação de triatomíneos. Embora não fosse determinado qual das vias, se placentária, leite, excreções ou contágio direto pelo qual o protozoário foi transmitido para os descendentes, chama-se atenção para a importância da manutenção de reservatórios da Doença de Chagas, mesmo na ausência de vetores invertebrados.The sucessive transmission of Trypanosoma cruzi among three generations of the guinea pig Cavia porcellus without the participation of triatomine bugs is verified. Although the mode of transmission, such as congenital, infected milk or other excretion or direct contagion was not defined, this maybe of importance in natural maintenance of reservoirs of T. cruzi without the invertebrate vectors.

  14. Detección de flavivirus en mosquitos (Diptera: Culicidae de la Isla de Pascua-Chile

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    Ximena Collao

    2015-02-01

    Full Text Available Los flavivirus son virus transmitidos por artrópodos, principalmente por especies de mosquitos de los géneros Aedes y Culex (Culicidae que se detectan principalmente en zonas tropicales y subtropicales. Los principales flavivirus de importancia en salud pública son el virus del dengue, del Nilo Occidental y fiebre amarilla, entre otros. En Chile continental no hay registro de flavivirus; sin embargo, se han detectado casos autóctonos de dengue en la Isla de Pascua desde el año 2002, al igual que la presencia de su vector Aedes aegypti. El objetivo de este estudio fue conocer la diversidad de los flavivirus presentes en mosquitos de la Isla de Pascua. Se analizaron 30 lotes de larvas de mosquitos de Hanga Roa, se realizó una RT-RPC anidada específica y genérica para flavivirus. Se detectaron 13 muestras positivas, identificándose dos flavivirus específicos de insectos, el virus cell fusing agent y otro relacionado con Kamiti river virus. Este es el primer estudio en Chile que evidencia la presencia de flavivirus en los vectores de la Isla de Pascua.

  15. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/ Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

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    Débora Cristina Santiago

    2005-06-01

    Full Text Available Seeds and seedlings of groundnut (Arachis hypogaea cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were transmitted by seeds and caused damping-off before and after seedling emergence.Sementes e plântulas de amendoim (Arachis hypogaea cv. Tatu foram avaliadas em condições de laboratório e casa de vegetação com o objetivo de detectar e identificar a presença de fungos patogênicos associados às sementes, além de determinar a patogenicidade e os danos por eles causados. Os fungos detectados foram Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. e Phoma sp.. Os fungos mais freqüentes foram A. niger e Penicillium sp.. Estes fungos foram transmitidos através das sementes e causaram tombamento de pré e pósemergência nas plântulas.

  16. La Geometría Entretejida

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    Mónica Lorena Micelli

    2011-02-01

    Full Text Available El presente artículo recopila el conocimiento geométrico plasmado a través de diseños textiles de diferentes pueblos nativos de América. Puede verse cómo en el arte del tejido en telar, se transmiten distintos conocimientos, muchos de ellos asociados a sus creencias, a ideas religiosas, estatus social o político. El marco, en el cual se ha realizado la presente investigación, es la etnomatemática, estudiando así los conocimientos matemáticos transmitidos en forma oral y plasmados en las prendas tejidas. Es así como seestudiarán los tejidos guatemaltecos, realizados por descendientes del pueblo Maya, transmitiendo en ellos parte de su cultura, también se incluirá un análisis de la obra textil incaica, para luego finalizar con los diseños realizados por el pueblo Mapuche que habitan el sur del territorio argentino

  17. The transmission of suprapylarian Leishmania by bite of experimentally infected sand flies (Diptera: Psychodidae A trasnmissão de Leishmania suprapilária pela picada do flebotomíneo infectado experimentalmente

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    L. Ryan

    1987-09-01

    Full Text Available Lutzomyia furcata transmitted Leishmania chagasi to a hamster 10 days after being experimentally fed on an infected spleen. An individual female Psychodopygus carrerai carrerai that had fed on a hamster lesion caused by Leishmania mexicana amazonensis transmitted this parasite 6 days later to another hamster. Transmission electron microscopy of this fly's head revealed a small number of degenerate promastigotes in the foregut, but only a few were attached.O protozoário Leishmania (L. chagasi foi transmitido experimentalmente a um hamster pela picada do flebotomíneo Lutzomyia furcata. Os insetos foram infectados através de uma membrana (pele de pinto, utilizando-se formas amastigotas provenientes do baço de um hamster infectado. O baço foi triturado em sangue de coelho. A L. (L. amazonensis foi transmitida a um hamster pela picada do flebotomíneo Psychodopygus c. carrerai, previamente alimentado em lesão de pele de um outro hamster infectado com o parasita. O exame desse flebotomíneo, através de microscópio eletrônico, revelou um número pequeno de flagelados degenerados, livres no lumen do intestino anterior.

  18. El Paleolítico en los dibujos animados: el universo de ficción prehistórica

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    Alberto Lombo Montañés

    2014-10-01

    Full Text Available El presente artículo es una indagación en el universo de ficción que en torno al paleolítico se ha transmitido a través de los dibujos animados. Se integran los dibujos animados de prehistoria dentro de las investigaciones presentes sobre cine histórico o prehistórico y se lleva a cabo una clasificación previa de los mismos. Por último, se analizan las películas de dibujos (la saga Ice Age y Los Croods más relevantes para el periodo paleolítico. Todo ello nos permite reflexionar acerca del papel de la prehistoria en el mundo de las imágenes cinematográficas y concluir que la relación entre ciencia y cine es más compleja de lo que parece. En conclusión se puede decir que el cine ha inventado su propia Edad de Piedra poblada de dinosaurios y Hombres de las cavernas no sin tener en cuenta a veces las investigaciones científicas.

  19. Ability of Aphis gossypii and Myzus persicae to Transmit Cucumber mosaic virus in Single and Mixed Infection with Two Potyviruses to Zucchini Squash Eficiência dos afídeos Aphis gossypii e Myzus persicae na transmissão do Cucumber mosaic virus em infecção simples e mista com dois Potyvirus para abobrinha de moita

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    Zayame Vegette Pinto

    2008-06-01

    Full Text Available The main objective of this work was to investigate the ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus (CMV singly and mixed with two potyviruses (Papaya ringspot virus - type W, PRSV-W and Zucchini yellow mosaic virus, ZYMV, to zucchini squash plants (Cucurbita pepo. The results showed that the potyviruses in general were more efficiently transmitted by both species of aphids as compared to CMV. The transmission of PRSV-W, ZYMV and CMV separately was more efficient than in mixture.O objetivo desse trabalho foi estudar a eficiência de Aphis gossypii e Myzus persicae na transmissão do vírus do mosaico do pepino (Cucumber mosaic virus, CMV, isoladamente e em mistura com duas espécies de potyvirus (Vírus do mosaico do mamoeiro = Papaya ringspot virus - type W, PRSV-W e Vírus do mosaico amarelo da abobrinha = Zucchini yellow mosaic virus, ZYMV, para planta-testes de abobrinha de moita (Cucurbita pepo. Os dois potyvirus em geral foram transmitidos com mais eficiência pelas duas espécies de afídeos do que o CMV. A transmissão do PRSV-W, ZYMV e CMV, separadamente, foi mais eficiente do que em mistura.

  20. Suscetibilidade de genótipos de feijão ao vírus-do-mosaico-dourado

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    Lemos Leandro Borges

    2003-01-01

    Full Text Available O objetivo deste trabalho foi avaliar a suscetibilidade de diversos genótipos de feijão ao vírus-do-mosaico-dourado (VMDF, transmitido pela mosca branca (Bemisia tabaci. A semeadura foi realizada na época da seca e das águas, com e sem aplicação do inseticida granulado Aldicarb (3,0 kg ha-1 do i.a. no sulco de semeadura. O delineamento experimental utilizado foi o de blocos casualizados disposto em esquema fatorial 14x2, representado por genótipos e inseticida, respectivamente, com quatro repetições. A maior infestação de mosca-branca e incidência do vírus ocorreu na época da seca, causando prejuízos à produção do feijoeiro. Os genótipos apresentaram diferentes graus de suscetibilidade ao vírus e ao inseto vetor. Os genótipos mais tolerantes foram IAPAR 57, IAPAR 65, IAPAR 72, Ônix, Aporé e 606 (5(214-17. A aplicação do inseticida sistêmico controla o vetor em ambas as épocas de cultivo, proporcionando aumentos da produtividade.

  1. Microclima e produção da uva de mesa 'Niagara Rosada' conduzida em espaldeira a céu aberto e em manjedoura na forma de "Y" sob cobertura de telado plástico

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    Mário José Pedro Júnior

    2011-10-01

    Full Text Available Foi desenvolvido um experimento em vinhedos de 'Niagara Rosada', conduzidos em espaldeira e em manjedoura na forma de Y com cobertura de telado plástico, visando a comparar as alterações microclimáticas induzidas pelo sistema de condução e uso de telado plástico, e o efeito na produtividade das videiras. Entre os parâmetros microclimáticos avaliados, apenas a radiação solar foi atenuada pelo telado plástico em cerca de 20%, acima do dossel, tendo sido transmitido para a altura do cacho, cerca de 41% da radiação solar no sistema em Y sob telado plástico e 21% no espaldeira. As temperaturas máximas e mínimas foram semelhantes em ambos os sistemas. A produção por planta e a massa dos cachos foram mais elevadas no sistema em Y sob telado plástico, em comparação ao espaldeira.

  2. Las industrias informativas: ¿tienen futuro?

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    Robert Picard

    2014-01-01

    Full Text Available La abundancia de información, la fragmentación y polarización de las au-diencias, el desarrollo del portafolio de ofertas, los cambios en las formasde comunicar y el propio debilitamiento de las compañías mediáticas obli-gan a las industrias informativas a repensar sus modelos de negocio, anteel descenso ineludible de los ingresos provenientes del pago de los usua-rios y la venta de publicidad. Algunos observadores confunden la difícil si-tuación de los medios informativos con el periodismo. Pero estos no sonsinónimos. El periodismo no es una forma de medio de comunicación; noes una plataforma de distribución; tampoco una industria o una compañía;ni un modelo de negocio, ni un trabajo. El periodismo es una actividad, unconjunto de prácticas mediante las cuales la información y el conocimien-to son obtenidos, procesados y transmitidos. Las perspectivas económicasy empresariales resultan importantes para responder interrogantes sobreel estado actual y futuro de las industrias informativas, porque sus desafíosfundamentales involucran nuevas estrategias de!nanciamiento, costos ypolíticas públicas que permitan hacer frente a los retos ineludibles que seplantean en el siglo XXI.

  3. Los Juegos Paralímpicos de Londres 2012: los Juegos de la inclusión

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    Miguel Ángel Torralba

    2012-12-01

    Full Text Available Los Juegos Paralímpicos de Londres 2012 han sido excelentes, sin duda los mejores juegos de la historia, pero si por algo se han caracterizado ha sido por el nivel de inclusión desarrollado. Siguiendo el modelo de los Juegos Olímpicos, los organizadores han dado rienda suelta a todos los valores que posee el pueblo británico y, como fundadores del movimiento paralímpico en Stoke Mandeville por el Dr. Guttmann, han sabido conjugar el espectáculo deportivo con la difusión de las capacidades de los deportistas discapacitados. Las proezas de los deportistas paralímpicos han transmitido a toda la sociedad, a través de los medios de comunicación, los valores que tan magníficamente encarnan. La participación ha sido numerosa, 166 países y mas de 4.000 atletas, lo que convierte estos Juegos en la segunda manifestación deportiva de la Tierra. España representa una de las potencias del movimiento Paralímpico, estando presente en los primeros puestos del medallero y siendo punteros en la difusión de modelos educativos y participando en las investigaciones auspiciadas por el Comité Paralímpico Internacional.

  4. Sugerencias a partir del análisis de sensibilidad de un modelo matemático de transmisión de chikungunya

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    David Requena

    2016-01-01

    Full Text Available La fiebre chikungunya afecta seriamente la salud de las personas, causando dolores articulares crónicos e incluso discapacidad. Es transmitida por picadura de los mosquitos Aedes aegypti y Aedes albopictus. Se han reportado brotes en diversas partes del mundo, incluyendo Latinoamérica. Estudios de modelamiento matemático de dichos brotes han calculado valores de parámetros epidemiológicos. En base a ellos, se elaboró un modelo matemático para simular un brote epidémico de chikungunya en una población local, transmitido desde una población vecina infectada. Mediante análisis de sensibilidad e incertidumbre se obtuvo que la tasa de transmisión mosquito-humano y humano-mosquito son las variables con mayor correlación con la cantidad de infectados, la cual se pierde significativamente a partir de los 60 días del primer caso en la población vecina. Se recomienda tomar esto en cuenta al planificar medidas de control sobre dichas variables como aislamiento de infectados, repartición de mosquiteros y repelentes, fumigación, entre otras.

  5. Artefatos de gênero na arte do barro: masculinidades e femininidades

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    MATOS SÔNIA MISSAGIA DE

    2001-01-01

    Full Text Available Este é um estudo sobre gênero na produção artística de peças de cerâmica em algumas comunidades de artesãos do Vale do Jequitinhonha, Minas Gerais, onde tradicionalmente apenas as mulheres chamadas "paneleiras" praticavam esse ofício, que era transmitido de mãe para filha. Naquela região, assim que a arte do barro se tornou uma fonte alternativa de renda, vários homens decidiram entrar para o ofício. A entrada de homens na arte do barro provocou reconfigurações nas relações de gênero. Um ponto muito importante a ser observado aqui é que apesar de tradicionalmente transmitida por mulheres e pela ação feminina, a arte do barro tem incorporado valores de masculinidade: ao se aproximarem de um ofício tradicionalmente feminino, os homens passaram a retirar dele recursos simbólicos de representação de masculinidade.

  6. Los manuales escritos por matronas europeas (1609-1710: el origen de cuidados humanizados en el embarazo

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    Carmen Martínez Rojo

    2014-06-01

    Full Text Available Objetivo principal: Analizar los cuidados administrados por matronas a mujeres en su embarazo en el siglo XVII transmitidos a través de los manuales escritos por las mismas parteras. Metodología: Metodología cualitativa, desde la hermenéutica y la historia cultural a través de técnicas etnográfico-narrativas mediante la lectura de cinco manuales (1609-1710, categorizando los textos siguiendo el modelo dialéctico de construcción-deconstrucción. Resultados principales: Obtuvimos como resultados la identificación de cuidados impregnados en creencias populares y el modo en que estos evolucionaron hacia la ciencia a través del diagnóstico, consejos higiénico-dietéticos, controles antenatales y atención a patologías. Conclusión principal: Concluimos que las matronas del siglo XVII se cohesionaron gracias al elemento estructural de los manuales en torno a valores de respeto a la fisiología de las mujeres y serán germen de una partería humanizada que les otorga identidad como colectivo profesional.

  7. ESTRATEGIAS GRAMATICALES DE EXPRESIÓN DE LA EVIDENCIALIDAD EN EL ESPAÑOL DE CHILE Grammatical strategies conveying evidentiality in Chilean Spanish

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    Carlos González Vergara

    2011-07-01

    Full Text Available Esta investigación tiene como objetivo determinar, en un corpus del español de Chile, la presencia, frecuencia y significados de estrategias gramaticales de expresión del significado evidencial. Los resultados muestran que para este dialecto el dominio semántico de la evidencialidad se transmite, al menos, por las conjugaciones de futuro simple y futuro perfecto, condicional simple y condicional perfecto, el pronombre uno, las formas de segunda persona singular, la construcción “determinante + tal + nombre propio” y estructuras dequeístas. Los significados evidenciales transmitidos son el reportativo/citativo, el de “fuente personal y acceso no privativo” y “fuente y acceso compartidos entre hablante y oyente”.This paper aims to determine, in a corpus of Chilean Spanish, the presence, frequency and meanings of evidential strategies. The results show that, for this dialect, the semantic domain of evidentiality is conveyed, at least, by the future simple and the future perfect, the conditional simple and the conditional perfect, the pronoun uno, the second person singular, the construction “determinant + tal + proper noun” and dequeísta structures. Evidential meanings conveyed are reportative / quotative, “personal source and universal access” and “source and access shared between speaker and listener”.

  8. Sexo oral e HIV entre homens que fazem sexo com homens Oral sex and HIV among men who have sex with men

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    Sergio Luis Funari

    2003-12-01

    Full Text Available A transmissão sexual do Vírus da Imunodeficiência Humana (HIV representa um problema de saúde pública em todo o mundo, e homens que fazem sexo com homens encontram-se sob risco diferenciado para essa infecção. Ainda que existam evidências suficientes para se afirmar que o HIV pode ser transmitido via sexo oral, a percepção desse risco é ambígua, e relaciona-se de forma paradoxal com mudanças de atitude. Novos modelos de percepção de risco devem ser desenvolvidos em diversas áreas de conhecimento para se alcançar compreensão aprofundada desse fenômeno.Sexual transmission of the human immunodeficiency virus (HIV continues to pose a public health problem worldwide. Men who have sex with men are still at differential risk of infection. Although there is evidence to claim that HIV can be transmitted by oral sex, the perception of this risk is ambiguous and relates paradoxically to behavior change. New models of risk perception must be developed in various areas of knowledge to obtain a fuller understanding of this phenomenon.

  9. Arranjo espacial do vira-cabeça do fumo em arapiraca, estado de alagoas

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    SILVA JEFFERSON N.

    2001-01-01

    Full Text Available O vira-cabeça do fumo (Nicotiana tabacum, causado pelo Groundnut ringspot virus (GRSV, do gênero Tospovirus, tem sido observado em alta incidência em determinadas áreas da região fumageira do agreste do estado de Alagoas. O arranjo espacial da doença foi analisado em duas áreas de plantio (A e B, formadas por quatro parcelas cada, localizadas no município de Arapiraca. As parcelas foram avaliadas semanalmente, sendo efetuado o mapeamento espacial de plantas sadias e com sintomas de vira-cabeça, bem como determinada a incidência da doença, representada pelo número de plantas com sintomas em relação ao total de plantas avaliadas. Pelas análises de "ordinary runs" e autocorrelação espacial, foi constatada a predominância do arranjo aleatório de plantas doentes, embora tenha sido detectada agregação em alguns casos. Embora não se descarte a possibilidade de mudas infetadas terem influenciado no arranjo da doença, a hipótese mais provável é que as infecções ocorreram principalmente pelo inóculo primário transmitido por tripes virulíferos entrando nas parcelas, oriundos de reservatórios externos.

  10. Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture

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    Luiz Tadeu M. Figueiredo

    1988-06-01

    Full Text Available A virus antigenic characterization methodology using an indirect method of antibody detection ELISA with virus-infected cultured cells as antigen and a micro virus neutralisation test using EIA (NT-EIA as an aid to reading were used for antigenic characterization of Jatobal (BeAn 423380. Jatobal virus was characterized as a Bunyaviridae, Bunyavirus genus, Simbu serogroup virus. ELISA using infected cultured cells as antigen is a sensitive and reliable method for identification of viruses and has many advantages over conventional antibody capture ELISA's and other tests: it eliminates solid phase coating with virus and laborious antigen preparation; it permits screening of large numbers of virus antisera faster and more easily than by CF, HAI, or plaque reduction NT. ELISA and NT using EIA as an aid to reading can be applicable to viruses which do not produce cytopathogenic effect. Both techniques are applicable to identification of viruses which grow in mosquito cells.A caracterização antigênica do vírus Jatobal (BeAn 423380 foi efetuada utilizando uma técnica de ELISA para deteccão de anticorpos que utiliza culturas celulares infectadas como antígeno e um micro teste de neutralização para vírus que utiliza o método imunoenzimático como auxiliar para a leitura dos resultados (NT-EIA. O vírus Jatobal foi caracterizado como um Bunyaviridae, gênero Bunyavirus, pertencente ao sorogrupo Simbu. A técnica de ELISA, utilizando culturas celulares infectadas como antígeno, trata-se de método sensível e confiável na identificação de agentes virais, possuindo muitas vantagens sobre ELISA convencionais e outros testes: elimina a preparação laboriosa de antígenos para o revestimento em fase sólida; permite que se teste de forma mais rápida e fácil que por CF, HAI e neutralização por redução de plaques um grande número de antisoros de vírus. ELISA e NT-EIA podem ser utilizados para a classificação de vírus que não produzem

  11. Diversity of viruses in Ixodes ricinus, and characterization of a neurotropic strain of Eyach virus.

    Science.gov (United States)

    Moutailler, S; Popovici, I; Devillers, E; Vayssier-Taussat, M; Eloit, M

    2016-05-01

    Ticks transmit more pathogens-including bacteria, parasites and viruses-than any other arthropod vector. Although the epidemiological status of many tick-borne bacteria is very well characterized, tick-borne viruses are still relatively under-studied. Recently, several novel tick-borne viruses have been isolated from human febrile illnesses following tick bites, indicating the existence of other potential new and unknown tick-borne viruses. We used high-throughput sequencing to analyse the virome of Ixodes ricinus, the main vector of tick-borne pathogens in Europe. The majority of collected viral sequences were assigned to two potentially novel Nairovirus and Phlebovirus viruses, with prevalence rates ranging from 3.95% to 23.88% in adults and estimated to be between 0.14% and 72.16% in nymphs. These viruses could not be isolated from the brains of inoculated immunocompromised mice, perhaps indicating that they are unable to infect vertebrates. Within the I. ricinus virome, we also identified contigs with >90% identity to the known Eyach virus. Initially isolated in the 1980s, this virus was indirectly associated with human disease, but had never been extensively studied. Eyach virus prevalence varied between 0.07% and 5.26% in ticks from the French Ardennes and Alsace regions. Eyach virus was successfully isolated following intracerebral inoculation of immunocompromised mice with Eyach virus-positive tick extracts. This virus was also able to multiply and persist in the blood of immunocompetent mice inoculated by intraperitoneal injection, and caused brain infections in three of nine juveniles, without any obvious deleterious effects. PMID:27158509

  12. N-Glycans on the Rift Valley Fever Virus Envelope Glycoproteins Gn and Gc Redundantly Support Viral Infection via DC-SIGN.

    Science.gov (United States)

    Phoenix, Inaia; Nishiyama, Shoko; Lokugamage, Nandadeva; Hill, Terence E; Huante, Matthew B; Slack, Olga A L; Carpio, Victor H; Freiberg, Alexander N; Ikegami, Tetsuro

    2016-01-01

    Rift Valley fever is a mosquito-transmitted, zoonotic disease that infects humans and ruminants. Dendritic cell specific intercellular adhesion molecule 3 (ICAM-3) grabbing non-integrin (DC-SIGN) acts as a receptor for members of the phlebovirus genus. The Rift Valley fever virus (RVFV) glycoproteins (Gn/Gc) encode five putative N-glycan sequons (asparagine (N)-any amino acid (X)-serine (S)/threonine (T)) at positions: N438 (Gn), and N794, N829, N1035, and N1077 (Gc). The N-glycosylation profile and significance in viral infection via DC-SIGN have not been elucidated. Gc N-glycosylation was first evaluated by using Gc asparagine (N) to glutamine (Q) mutants. Subsequently, we generated a series of recombinant RVFV MP-12 strain mutants, which encode N-to-Q mutations, and the infectivity of each mutant in Jurkat cells stably expressing DC-SIGN was evaluated. Results showed that Gc N794, N1035, and N1077 were N-glycosylated but N829 was not. Gc N1077 was heterogeneously N-glycosylated. RVFV Gc made two distinct N-glycoforms: "Gc-large" and "Gc-small", and N1077 was responsible for "Gc-large" band. RVFV showed increased infection of cells expressing DC-SIGN compared to cells lacking DC-SIGN. Infection via DC-SIGN was increased in the presence of either Gn N438 or Gc N1077. Our study showed that N-glycans on the Gc and Gn surface glycoproteins redundantly support RVFV infection via DC-SIGN. PMID:27223297

  13. N-Glycans on the Rift Valley Fever Virus Envelope Glycoproteins Gn and Gc Redundantly Support Viral Infection via DC-SIGN

    Directory of Open Access Journals (Sweden)

    Inaia Phoenix

    2016-05-01

    Full Text Available Rift Valley fever is a mosquito-transmitted, zoonotic disease that infects humans and ruminants. Dendritic cell specific intercellular adhesion molecule 3 (ICAM-3 grabbing non-integrin (DC-SIGN acts as a receptor for members of the phlebovirus genus. The Rift Valley fever virus (RVFV glycoproteins (Gn/Gc encode five putative N-glycan sequons (asparagine (N–any amino acid (X–serine (S/threonine (T at positions: N438 (Gn, and N794, N829, N1035, and N1077 (Gc. The N-glycosylation profile and significance in viral infection via DC-SIGN have not been elucidated. Gc N-glycosylation was first evaluated by using Gc asparagine (N to glutamine (Q mutants. Subsequently, we generated a series of recombinant RVFV MP-12 strain mutants, which encode N-to-Q mutations, and the infectivity of each mutant in Jurkat cells stably expressing DC-SIGN was evaluated. Results showed that Gc N794, N1035, and N1077 were N-glycosylated but N829 was not. Gc N1077 was heterogeneously N-glycosylated. RVFV Gc made two distinct N-glycoforms: “Gc-large” and “Gc-small”, and N1077 was responsible for “Gc-large” band. RVFV showed increased infection of cells expressing DC-SIGN compared to cells lacking DC-SIGN. Infection via DC-SIGN was increased in the presence of either Gn N438 or Gc N1077. Our study showed that N-glycans on the Gc and Gn surface glycoproteins redundantly support RVFV infection via DC-SIGN.

  14. Diversity of viruses in Ixodes ricinus, and characterization of a neurotropic strain of Eyach virus

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    S. Moutailler

    2016-05-01

    Full Text Available Ticks transmit more pathogens—including bacteria, parasites and viruses—than any other arthropod vector. Although the epidemiological status of many tick-borne bacteria is very well characterized, tick-borne viruses are still relatively under-studied. Recently, several novel tick-borne viruses have been isolated from human febrile illnesses following tick bites, indicating the existence of other potential new and unknown tick-borne viruses. We used high-throughput sequencing to analyse the virome of Ixodes ricinus, the main vector of tick-borne pathogens in Europe. The majority of collected viral sequences were assigned to two potentially novel Nairovirus and Phlebovirus viruses, with prevalence rates ranging from 3.95% to 23.88% in adults and estimated to be between 0.14% and 72.16% in nymphs. These viruses could not be isolated from the brains of inoculated immunocompromised mice, perhaps indicating that they are unable to infect vertebrates. Within the I. ricinus virome, we also identified contigs with >90% identity to the known Eyach virus. Initially isolated in the 1980s, this virus was indirectly associated with human disease, but had never been extensively studied. Eyach virus prevalence varied between 0.07% and 5.26% in ticks from the French Ardennes and Alsace regions. Eyach virus was successfully isolated following intracerebral inoculation of immunocompromised mice with Eyach virus-positive tick extracts. This virus was also able to multiply and persist in the blood of immunocompetent mice inoculated by intraperitoneal injection, and caused brain infections in three of nine juveniles, without any obvious deleterious effects.

  15. Small RNA profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected Nicotiana benthamiana.

    Science.gov (United States)

    Margaria, Paolo; Miozzi, Laura; Rosa, Cristina; Axtell, Michael J; Pappu, Hanu R; Turina, Massimo

    2015-10-01

    Tospoviruses are plant-infecting viruses belonging to the family Bunyaviridae. We used a collection of wild-type, phylogenetically distinct tomato spotted wilt virus isolates and related silencing-suppressor defective mutants to study the effects on the small RNA (sRNA) accumulation during infection of Nicotiana benthamiana. Our data showed that absence of a functional silencing suppressor determined a marked increase of the total amount of viral sRNAs (vsRNAs), and specifically of the 21 nt class. We observed a common under-representation of vsRNAs mapping to the intergenic region of S and M genomic segments, and preferential mapping of the reads against the viral sense open reading frames, with the exception of the NSs gene. The NSs-mutant strains showed enrichment of NSm-derived vsRNA compared to the expected amount based on gene size. Analysis of 5' terminal nucleotide preference evidenced a significant enrichment in U for the 21 nt- and in A for 24 nt-long endogenous sRNAs in all the samples. Hotspot analysis revealed a common abundant accumulation of reads at the 5' end of the L segment, mostly in the antiviral sense, for the NSs-defective isolates, suggesting that absence of the silencing suppressor can influence preferential targeting of the viral genome. PMID:26047586

  16. [Serological monitoring of arbovirus infections in the estuary of the Kuban River (the 2006-2007 data)].

    Science.gov (United States)

    L'vov, D K; Shchelkanov, M Iu; Kolobukhina, L V; L'vov, D N; Galkina, I V; Aristova, V A; Morozova, T N; Proshina, E S; Kulikov, A G; Kogdenko, N V; Andronova, O V; Pronin, N I; Shevkoplias, V N; Fontanetskiĭ, A S; Vlasov, N A; Nepoklonov, E A

    2008-01-01

    Solid-phase enzyme immunoassay, neutralization test, and the hemagglutination-inhibition test were used to study the sera from human beings (152 samples), agricultural animals (n = 77), hares (n = 3), and wild birds (n = 69), collected in 2006-2007 in the Kuban River estuary (Temryuk District, Krasnodar Territory). There were specific antibodies against viruses of West Nile (WH), tick-borne encephalitis (TBE) (Flaviviridae, Flavivirus), Sindbis (Togaviridae, Alphavirus), the antigenic complex of California, Batai (Bunyaviridae, Orthobunyavirus), Dhori (Orthomyxoviridae, Thogotovirus). The findings suggest the presence of arboviruses from 6 transmitting mosquitoes and ticks in the study area and human infection by the viruses of the antigenic complex of California (20-47%), Batai (3-15%), West Nile (3-12%), Dhori (2%). The index agricultural animals (horses, cattle) were observed to have specific antibodies to the viruses of WN (8-15%), TBE (0-2%), Sindbis (2-9%), the antigenic complex of California (27-54%). Out of the representatives of the wild fauna, virus-neutralizing antibodies to Sindbis virus were found in European hares (Lepus europaeus), California complex virus in gulls (Larus argentatus) and terns (Sterna hirundo), WN and Sindbis viruses in herons (Ardea purpurea), and WN and California complex viruses in bald-coots (Fulica atra). PMID:18756814

  17. Phylogeographic analysis of hemorrhagic fever with renal syndrome patients using multiplex PCR-based next generation sequencing

    Science.gov (United States)

    Kim, Won-Keun; Kim, Jeong-Ah; Song, Dong Hyun; Lee, Daesang; Kim, Yong Chul; Lee, Sook-Young; Lee, Seung-Ho; No, Jin Sun; Kim, Ji Hye; Kho, Jeong Hoon; Gu, Se Hun; Jeong, Seong Tae; Wiley, Michael; Kim, Heung-Chul; Klein, Terry A.; Palacios, Gustavo; Song, Jin-Won

    2016-01-01

    Emerging and re-emerging infectious diseases caused by RNA viruses pose a critical public health threat. Next generation sequencing (NGS) is a powerful technology to define genomic sequences of the viruses. Of particular interest is the use of whole genome sequencing (WGS) to perform phylogeographic analysis, that allows the detection and tracking of the emergence of viral infections. Hantaviruses, Bunyaviridae, cause hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) in humans. We propose to use WGS for the phylogeographic analysis of human hantavirus infections. A novel multiplex PCR-based NGS was developed to gather whole genome sequences of Hantaan virus (HTNV) from HFRS patients and rodent hosts in endemic areas. The obtained genomes were described for the spatial and temporal links between cases and their sources. Phylogenetic analyses demonstrated geographic clustering of HTNV strains from clinical specimens with the HTNV strains circulating in rodents, suggesting the most likely site and time of infection. Recombination analysis demonstrated a genome organization compatible with recombination of the HTNV S segment. The multiplex PCR-based NGS is useful and robust to acquire viral genomic sequences and may provide important ways to define the phylogeographical association and molecular evolution of hantaviruses. PMID:27221218

  18. The high genetic variation of viruses of the genus Nairovirus reflects the diversity of their predominant tick hosts

    International Nuclear Information System (INIS)

    The genus Nairovirus (family Bunyaviridae) contains seven serogroups consisting of 34 predominantly tick-borne viruses, including several associated with severe human and livestock diseases [e.g., Crimean Congo hemorrhagic fever (CCHF) and Nairobi sheep disease (NSD), respectively]. Before this report, no comparative genetic studies or molecular detection assays had been developed for this virus genus. To characterize at least one representative from each of the seven serogroups, reverse transcriptase-polymerase chain reaction (RT-PCR) primers targeting the L polymerase-encoding region of the RNA genome of these viruses were successfully designed based on conserved amino acid motifs present in the predicted catalytic core region. Sequence analysis showed the nairoviruses to be a highly diverse group, exhibiting up to 39.4% and 46.0% nucleotide and amino acid identity differences, respectively. Virus genetic relationships correlated well with serologic groupings and with tick host associations. Hosts of these viruses include both the hard (family Ixodidae) and soft (family Argasidae) ticks. Virus phylogenetic analysis reveals two major monophyletic groups: hard tick and soft tick-vectored viruses. In addition, viruses vectored by Ornithodoros, Carios, and Argas genera ticks also form three separate monophyletic lineages. The striking similarities between tick and nairovirus phylogenies are consistent with possible coevolution of the viruses and their tick hosts. Fossil and phylogenetic data placing the hard tick-soft tick divergence between 120 and 92 million years ago suggest an ancient origin for viruses of the genus Nairovirus

  19. Rift Valley fever in Central Africa: Serological evidence and virus antigen detection in cattle in the Democratic Republic of Congo

    International Nuclear Information System (INIS)

    In order to assess the disease status within the cattle population in the Democratic Republic of Congo, a survey was carried out using two investigation strategies, i.e. 1) anti - RVF virus (RVFV) Ig G antibodies (Abs) detection, and 2) virus detection. Five provinces were selected due to their high bovine exploitation activity. 962 sera samples randomly collected in 26 locations from the 5 mentioned provinces were tested exploiting the recombinant N protein indirect ELISA (I - ELISA) for antibodies detection and prevalence estimate and for virus detection, two diagnostic methods were exploited: a) - virus antigen (Ag) detection in tissues using the immunoperoxidase (IMP) staining with Avidin Biotin Complex (ABC) technique and, b) -virus cDNA detection using RT- PCR. Only some tissues from syndromically suspected cases from one location with the highest prevalence (20%) were analysed for virus detection. The structural, nucleo . capsid (N) protein that the small segment (S) of the RVFV genome encodes with forms the key antigen targeted in all the 3 diagnostic methods. As a matter of fact, N protein is the most immunocompetent and most expressed protein of RVFV; it is also the highest conserved protein amongst members of the Bunyaviridae family

  20. Analysis of N-linked glycosylation of hantaan virus glycoproteins and the role of oligosaccharide side chains in protein folding and intracellular trafficking.

    Science.gov (United States)

    Shi, Xiaohong; Elliott, Richard M

    2004-05-01

    The membrane glycoproteins Gn and Gc of Hantaan virus (HTNV) (family Bunyaviridae) are modified by N-linked glycosylation. The glycoproteins contain six potential sites for the attachment of N-linked oligosaccharides, five sites on Gn and one on Gc. The properties of the N-linked oligosaccharide chains were analyzed by treatment with endoglycosidase H, peptide:N-glycosidase F, tunicamycin, and deoxynojirimycin and were confirmed to be completely of the high-mannose type. Ten glycoprotein gene mutants were constructed by site-directed mutagenesis, including six single N glycosylation site mutants and four double-site mutants. We determined that four sites (N134, -235, -347, and -399) on Gn and the only site (N928) on Gc in their ectodomains are utilized, whereas the fifth site on Gn (N609), which faces the cytoplasm, is not glycosylated. The importance of individual N-oligosaccharide chains varied with respect to folding and intracellular transport. The oligosaccharide chain on residue N134 was found to be crucial for protein folding, whereas single mutations at the other glycosylation sites were better tolerated. Mutation at glycosylation sites N235 and N399 together resulted in Gn misfolding. The endoplasmic reticulum chaperones calnexin and calreticulin were found to be involved in HTNV glycoprotein folding. Our data demonstrate that N-linked glycosylation of HTNV glycoproteins plays important and differential roles in protein folding and intracellular trafficking. PMID:15113920

  1. What Do We Know about How Hantaviruses Interact with Their Different Hosts?

    Science.gov (United States)

    Ermonval, Myriam; Baychelier, Florence; Tordo, Noël

    2016-01-01

    Hantaviruses, like other members of the Bunyaviridae family, are emerging viruses that are able to cause hemorrhagic fevers. Occasional transmission to humans is due to inhalation of contaminated aerosolized excreta from infected rodents. Hantaviruses are asymptomatic in their rodent or insectivore natural hosts with which they have co-evolved for millions of years. In contrast, hantaviruses cause different pathologies in humans with varying mortality rates, depending on the hantavirus species and its geographic origin. Cases of hemorrhagic fever with renal syndrome (HFRS) have been reported in Europe and Asia, while hantavirus cardiopulmonary syndromes (HCPS) are observed in the Americas. In some cases, diseases caused by Old World hantaviruses exhibit HCPS-like symptoms. Although the etiologic agents of HFRS were identified in the early 1980s, the way hantaviruses interact with their different hosts still remains elusive. What are the entry receptors? How do hantaviruses propagate in the organism and how do they cope with the immune system? This review summarizes recent data documenting interactions established by pathogenic and nonpathogenic hantaviruses with their natural or human hosts that could highlight their different outcomes. PMID:27529272

  2. Increased Mean Platelet Volume in Patients with Crimean Congo Hemorrhagic Fever

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    Ayse Ižnci

    2014-03-01

    Full Text Available Aim: The Crimean%u2013Congo hemorrhagic fever virus( CCHFV is classified within the Nairovirus genus in the Bunyaviridae family. Mean platelet volume (MPV is a biomarker of platelet function and activity. Platelet activity and aggregation capacity can be determined by measuring mean platelet volume (MPV. MPV is a parameter that has been associated with the diseases in many publications. The aim of this study is to investigate the change in the MPV values in patients with CCHF. Material and Method: The study was designed in the Artvin State Hospital. We recruited 74 patients with CCHF Thirty healthy individuals were included as a control group whom were age and gender matched with CCHF patients. We compared the levels of MPV, platelet counts, age, gender, hemoglobin, white blood cell between CCHF patients with (and healthy individuals. Data were analyzed using the Statistical Package for the Social Sciences (SPSS Inc., Chicago, IL, USA version 15. The t-test or Mann-Whitney U test was used. Level of statistical significance was considered p < 0.05 and the results were presented as mean ± standard deviation. Results: There was no significant difference between the patients and controls in terms of age and gender. The mean platelet volume was 8.98±1.13 in CCHF group and 8.15±0.53 in the control group. There was a statiscically significant difference between the groups(p

  3. Mapping of the interaction domains of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein.

    Science.gov (United States)

    Levingston Macleod, Jesica M; Marmor, Hannah; García-Sastre, Adolfo; Frias-Staheli, Natalia

    2015-03-01

    Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the genus Nairovirus of the family Bunyaviridae, that can cause severe haemorrhagic fever in humans, with mortality rates above 30  %. CCHFV is the most widespread of the tick-borne human viruses and it is endemic in areas of central Asia, the Middle East, Africa and southern Europe. Its viral genome consists of three negative-sense RNA segments. The large segment (L) encodes a viral RNA-dependent RNA polymerase (L protein), the small segment (S) encodes the nucleocapsid protein (N protein) and the medium segment (M) encodes the envelope proteins. The N protein of bunyaviruses binds genomic RNA, forming the viral ribonucleoprotein (RNP) complex. The L protein interacts with these RNP structures, allowing the initiation of viral replication. The N protein also interacts with actin, although the regions and specific residues involved in these interactions have not yet been described. Here, by means of immunoprecipitation and immunofluorescence assays, we identified the regions within the CCHFV N protein implicated in homo-oligomerization and actin binding. We describe the interaction of the N protein with the CCHFV L protein, and identify the N- and C-terminal regions within the L protein that might be necessary for the formation of these N-L protein complexes. These results may guide the development of potent inhibitors of these complexes that could potentially block CCHFV replication. PMID:25389186

  4. Mapping of the interaction domains of the Crimean–Congo hemorrhagic fever virus nucleocapsid protein

    Science.gov (United States)

    Macleod, Jesica M. Levingston; Marmor, Hannah; Frias-Staheli, Natalia

    2015-01-01

    Crimean–Congo hemorrhagic fever virus (CCHFV) is a member of the genus Nairovirus of the family Bunyaviridae, that can cause severe haemorrhagic fever in humans, with mortality rates above 30 %. CCHFV is the most widespread of the tick-borne human viruses and it is endemic in areas of central Asia, the Middle East, Africa and southern Europe. Its viral genome consists of three negative-sense RNA segments. The large segment (L) encodes a viral RNA-dependent RNA polymerase (L protein), the small segment (S) encodes the nucleocapsid protein (N protein) and the medium segment (M) encodes the envelope proteins. The N protein of bunyaviruses binds genomic RNA, forming the viral ribonucleoprotein (RNP) complex. The L protein interacts with these RNP structures, allowing the initiation of viral replication. The N protein also interacts with actin, although the regions and specific residues involved in these interactions have not yet been described. Here, by means of immunoprecipitation and immunofluorescence assays, we identified the regions within the CCHFV N protein implicated in homo-oligomerization and actin binding. We describe the interaction of the N protein with the CCHFV L protein, and identify the N- and C-terminal regions within the L protein that might be necessary for the formation of these N–L protein complexes. These results may guide the development of potent inhibitors of these complexes that could potentially block CCHFV replication. PMID:25389186

  5. Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF virus glycoproteins

    Directory of Open Access Journals (Sweden)

    Fernando Lisa

    2005-04-01

    Full Text Available Abstract Background Crimean-Congo Hemorrhagic Fever virus (CCHFV, a member of the genus Nairovirus, family Bunyaviridae, is a tick-borne pathogen causing severe disease in humans. To better understand the CCHFV life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of the glycoproteins, which are encoded by the M genome segment. Results Following determination of the complete genome sequence of the CCHFV reference strain IbAr10200, we generated expression plasmids for the individual expression of the glycoproteins GN and GC, using CMV- and chicken β-actin-driven promoters. The cellular localization of recombinantly expressed CCHFV glycoproteins was compared to authentic glycoproteins expressed during virus infection using indirect immunofluorescence assays, subcellular fractionation/western blot assays and confocal microscopy. To further elucidate potential intracellular targeting/retention signals of the two glycoproteins, GFP-fusion proteins containing different parts of the CCHFV glycoprotein were analyzed for their intracellular targeting. The N-terminal glycoprotein GN localized to the Golgi complex, a process mediated by retention/targeting signal(s in the cytoplasmic domain and ectodomain of this protein. In contrast, the C-terminal glycoprotein GC remained in the endoplasmic reticulum but could be rescued into the Golgi complex by co-expression of GN. Conclusion The data are consistent with the intracellular targeting of most bunyavirus glycoproteins and support the general model for assembly and budding of bunyavirus particles in the Golgi compartment.

  6. Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures

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    Hedlund Kjell-Olof

    2011-02-01

    Full Text Available Abstract Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV, of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i electron microscopy for the quantification of virus particles, (ii quantitative real time PCR for the quantification of genomes, and (iii determination of focus forming units by coating fluorescent antibodies to infected cell monolayers for the quantification of virus infectivity. The comparative analysis revealed that filovirus and RVFV replication results in a surplus of genomes but varying degrees of packaging efficiency and infectious particles. More efficient replication and packaging was observed for Lassa virus, and Dengue virus resulting in a better yield of infectious particles while, YFV turned out to be most efficient with only 4 particles inducing one FFU. For Crimean-Congo haemorrhagic fever virus (CCHFV a surplus of empty shells was observed with only one in 24 particles equipped with a genome. The complete particles turned out to be extraordinarily infectious.

  7. Assessment of Inhibitors of Pathogenic Crimean-Congo Hemorrhagic Fever Virus Strains Using Virus-Like Particles.

    Science.gov (United States)

    Zivcec, Marko; Metcalfe, Maureen G; Albariño, César G; Guerrero, Lisa W; Pegan, Scott D; Spiropoulou, Christina F; Bergeron, Éric

    2015-12-01

    Crimean-Congo hemorrhagic fever (CCHF) is an often lethal, acute inflammatory illness that affects a large geographic area. The disease is caused by infection with CCHF virus (CCHFV), a nairovirus from the Bunyaviridae family. Basic research on CCHFV has been severely hampered by biosafety requirements and lack of available strains and molecular tools. We report the development of a CCHF transcription- and entry-competent virus-like particle (tecVLP) system that can be used to study cell entry and viral transcription/replication over a broad dynamic range (~4 orders of magnitude). The tecVLPs are morphologically similar to authentic CCHFV. Incubation of immortalized and primary human cells with tecVLPs results in a strong reporter signal that is sensitive to treatment with neutralizing monoclonal antibodies and by small molecule inhibitors of CCHFV. We used glycoproteins and minigenomes from divergent CCHFV strains to generate tecVLPs, and in doing so, we identified a monoclonal antibody that can prevent cell entry of tecVLPs containing glycoproteins from 3 pathogenic CCHFV strains. In addition, our data suggest that different glycoprotein moieties confer different cellular entry efficiencies, and that glycoproteins from the commonly used strain IbAr10200 have up to 100-fold lower ability to enter primary human cells compared to glycoproteins from pathogenic CCHFV strains. PMID:26625182

  8. An overview of Crimean- Congo Hemorrhagic Fever in Iran

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    Sadegh Chinikar

    2009-01-01

    Full Text Available Crimean- Congo Hemorrhagic Fever (CCHF is a viral zoonotic tick-born disease with a mortality rate of up to 50% in humans. After a short incubation period, the disease is characterized by sudden fever, chills, severe headache, dizziness, back, and abdominal pain. Additional symptoms can include nausea, vomiting, diarrhea, neuropsychiatric, and cardiovascular changes. In severe cases, hemorrhagic manifestations, ranging from petechiae to large areas of ecchymosis develop. The CCHF Virus (CCHFV is from the genus Nairovirus and family Bunyaviridae. CCHFV is transmitted to humans by the bite of infected tick and by direct contact with blood or tissue from infected humans and livestock. In addition to zoonotic transmission, CCHFV can be spread from person to person and is one of the rare hemorrhagic fever viruses able to cause nosocomial outbreaks in hospitals. CCHF is a public health problem in many regions of the world e.g Eastern Europe, Asia, Middle East, and Africa. The history of CCHF in Iran shows that the disease has been detected in Iran since 1970. From 1970 to 1978 some scientists worked on serology and epidemiology of this disease in humans and livestock in Iran. Since 1999 , establishment of a surveillance and laboratory detection system on viral hemorrhagic fevers particularly on CCHF has had benefits. One of which is the fact that a mortality rate approaching 20% in the year 2000 remarkably dropped to 6% in the year 2007.

  9. Development and evaluation of a real-time RT-qPCR for detection of Crimean-Congo hemorrhagic fever virus representing different genotypes.

    Science.gov (United States)

    Jääskeläinen, Anne J; Kallio-Kokko, Hannimari; Ozkul, Aykut; Bodur, Hurrem; Korukruoglu, Gulay; Mousavi, Mehrdad; Pranav, Patel; Vaheri, Antti; Mirazimi, Ali; Vapalahti, Olli

    2014-12-01

    Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic disease caused by a nairovirus belonging to family Bunyaviridae. The CCHF virus (CCHFV) can be transmitted to humans by Hyalomma ticks as well as by direct contact with infected body fluids or tissues from viremic livestock or humans. Our aim was to set up a fast RT-qPCR for detection of the different CCHFV genotypes in clinical samples, including an inactivation step to make the sample handling possible in lower biosafety levels (BSL) than BSL-4. This method was evaluated against commercial reference assays and international External Quality Assessment (EQA) samples. The analytical limit of detection for the developed CCHFV-S RT-qPCR was 11 CCHFV genomes per reaction. After exclusion of four dubious samples, we studied 38 CCHFV-positive samples (using reference tests) of which 38 were found positive by CCHFV-S RT-qPCR, suggesting a sensitivity of 100%. CCHFV-S RT q-PCR detected all eight different CCHFV strains representing five different CCHFV genotypes. In conclusion, the CCHFV-S RT-qPCR described in this study was evaluated using various sources of CCHFV samples and shown to be an accurate tool to detect human CCHFV infection caused by different genotypes of the virus. PMID:25514124

  10. A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

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    Elbashir Mustafa I

    2011-06-01

    Full Text Available Abstract Background Crimean-Congo hemorrhagic fever (CCHF, a tick-borne disease caused by Crimean-Congo hemorrhagic fever virus (CCHFV, is a member of the genus Nairovirus in the family Bunyaviridae. Recently, CCHFV has been reported as an important emerging infectious viral pathogen in Sudan. Sporadic cases and multiple CCHF outbreaks, associated with nosocomial chain of transmission, have been reported in the Kordufan region of Sudan. Aims To confirm CCHF in an index patient and attending physician in North Kordufan region, Sudan, and to provide some information on virus genetic lineages. Methods Antibody captured ELISA, reverse transcription PCR, partial S segment sequences of the virus and subsequent phylogenetic analysis were used to confirm the CCHFV infection and to determine the virus genetic lineages. Results CCHF was confirmed by monitoring specific IgM antibody and by detection of the viral genome using RT-PCR. Treatment with oral ribavirin, replacement with fluid therapy, blood transfusion and administration of platelets concentrate resulted in rapid improvement of the health condition of the female physician. Phylogenetic analysis of the partial S segment sequences of the 2 CCHFV indicates that both strains are identical and belong to Group III virus lineage, which includes viruses from Africa including, Sudan, Mauritania, South Africa and Nigeria. Conclusion Further epidemiologic studies including, CCHFV complete genome analysis and implementation of improved surveillance are urgently needed to better predict and respond to CCHF outbreaks in the Kordufan region, Sudan.

  11. Molecular epidemiology of Crimean-Congo hemorrhagic fever virus genome isolated from ticks of Hamadan province of Iran

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    F. Tahmasebi , S.M. Ghiasi , E. Mostafavi , M. Moradi , N. Piazak , A. Mozafari , A. Haeri , A.R. Fooks , S. Chinikar

    2010-12-01

    Full Text Available Background & objectives: Crimean-Congo hemorrhagic fever (CCHF virus is a tick-borne memberof the genus Nairovirus, family Bunyaviridae. CCHFV has been isolated from at least 31 differenttick species. The virus is transmitted through the bite of an infected tick, or by direct contact withCCHFV-infected patients or the products of infected livestock. This study was undertaken to studythe genetic relationship and distribution of CCHFV in the tick population of Hamadan province ofIran.Method: In this study, RT-PCR has been used for detection of the CCHFV genome.Results: This genome was detected in 19.2% of the ticks collected from livestock of differentregions of the Hamadan province in western Iran. The infected species belonged to Hyalommadetritum, H. anatolicum, Rhipicephalus sanguineus and Argas reflexus. With one exception, geneticanalysis of the virus genome isolates showed high sequence identity to each other. Even thoughthey clustered in the same group with the strain circulating in Iran, they had a closer relationshipto the Matin strain.Interpretation & conclusion: Vector control programs should be applied for reducing populationdensity of potential tick vectors in this province. Further surveys are indicated in this region toprovide a better view of the distribution and epidemiology of the virus.

  12. The full genome sequence of three strains of Jamestown Canyon virus and their pathogenesis in mice or monkeys

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    Murphy Brian R

    2011-03-01

    Full Text Available Abstract Background Jamestown Canyon virus (JCV, family Bunyaviridae, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of the northern hemisphere contains JCV or similar variants. Results We have completed the sequence of three isolates of JCV collected in geographically diverse locations over a 57 year time span. The nucleotide identity for the three strains is 90, 83, and 85% for the S, M, and L segments respectively whereas the percent identify for the predicted amino acid sequences of the N, NSS, M poly, GN, NSM, GC, and L proteins was 97, 91, 94, 98, 91, 94, and 97%, respectively. In Swiss Webster mice, each JCV isolate exhibits low neuroinvasiveness but high infectivity. Two of the three JCV isolates were highly neurovirulent after IC inoculation whereas one isolate, JCV/03/CT, exhibited low neurovirulence. In rhesus monkeys, JCV infection is accompanied by a low-titered viremia, lack of clinical disease, but a robust neutralizing antibody response. Conclusions The first complete sequence of JCV is reported for three separate isolates, and a relatively high level of amino acid sequence conservation was observed even for viruses isolated 57 years apart indicating that the virus is in relative evolutionary stasis. JCV is highly infectious for mice and monkeys, and these animals, especially mice, represent useful experimental hosts for further study.

  13. A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.

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    Laurent Dacheux

    Full Text Available The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because they cause severe human diseases. However, little is known about the diversity of the global population of viruses found in bats (virome. We determined the viral diversity of five different French insectivorous bat species (nine specimens in total in close contact with humans. Sequence-independent amplification, high-throughput sequencing with Illumina technology and a dedicated bioinformatics analysis pipeline were used on pooled tissues (brain, liver and lungs. Comparisons of the sequences of contigs and unassembled reads provided a global taxonomic distribution of virus-related sequences for each sample, highlighting differences both within and between bat species. Many viral families were present in these viromes, including viruses known to infect bacteria, plants/fungi, insects or vertebrates, the most relevant being those infecting mammals (Retroviridae, Herpesviridae, Bunyaviridae, Poxviridae, Flaviviridae, Reoviridae, Bornaviridae, Picobirnaviridae. In particular, we detected several new mammalian viruses, including rotaviruses, gammaretroviruses, bornaviruses and bunyaviruses with the identification of the first bat nairovirus. These observations demonstrate that bats naturally harbor viruses from many different families, most of which infect mammals. They may therefore constitute a major reservoir of viral diversity that should be analyzed carefully, to determine the role played by bats in the spread of zoonotic viral infections.

  14. Virus like particle-based vaccines against emerging infectious disease viruses.

    Science.gov (United States)

    Liu, Jinliang; Dai, Shiyu; Wang, Manli; Hu, Zhihong; Wang, Hualin; Deng, Fei

    2016-08-01

    Emerging infectious diseases are major threats to human health. Most severe viral disease outbreaks occur in developing regions where health conditions are poor. With increased international travel and business, the possibility of eventually transmitting infectious viruses between different countries is increasing. The most effective approach in preventing viral diseases is vaccination. However, vaccines are not currently available for numerous viral diseases. Virus-like particles (VLPs) are engineered vaccine candidates that have been studied for decades. VLPs are constructed by viral protein expression in various expression systems that promote the selfassembly of proteins into structures resembling virus particles. VLPs have antigenicity similar to that of the native virus, but are non-infectious as they lack key viral genetic material. VLP vaccines have attracted considerable research interest because they offer several advantages over traditional vaccines. Studies have shown that VLP vaccines can stimulate both humoral and cellular immune responses, which may offer effective antiviral protection. Here we review recent developments with VLP-based vaccines for several highly virulent emerging or re-emerging infectious diseases. The infectious agents discussed include RNA viruses from different virus families, such as the Arenaviridae, Bunyaviridae, Caliciviridae, Coronaviridae, Filoviridae, Flaviviridae, Orthomyxoviridae, Paramyxoviridae, and Togaviridae families. PMID:27405928

  15. Phylogenetic analysis of a newfound bat-borne hantavirus supports a laurasiatherian host association for ancestral mammalian hantaviruses.

    Science.gov (United States)

    Witkowski, Peter T; Drexler, Jan F; Kallies, René; Ličková, Martina; Bokorová, Silvia; Mananga, Gael D; Szemes, Tomáš; Leroy, Eric M; Krüger, Detlev H; Drosten, Christian; Klempa, Boris

    2016-07-01

    Until recently, hantaviruses (family Bunyaviridae) were believed to originate from rodent reservoirs. However, genetically distinct hantaviruses were lately found in shrews and moles, as well as in bats from Africa and Asia. Bats (order Chiroptera) are considered important reservoir hosts for emerging human pathogens. Here, we report on the identification of a novel hantavirus, provisionally named Makokou virus (MAKV), in Noack's Roundleaf Bat (Hipposideros ruber) in Gabon, Central Africa. Phylogenetic analysis of the genomic l-segment showed that MAKV was the most closely related to other bat-borne hantaviruses and shared a most recent common ancestor with the Asian hantaviruses Xuan Son and Laibin. Breakdown of the virus load in a bat animal showed that MAKV resembles rodent-borne hantaviruses in its organ distribution in that it predominantly occurred in the spleen and kidney; this provides a first insight into the infection pattern of bat-borne hantaviruses. Ancestral state reconstruction based on a tree of l gene sequences of all relevant hantavirus lineages was combined with phylogenetic fossil host hypothesis testing, leading to a statistically significant rejection of the mammalian superorder Euarchontoglires (including rodents) but not the superorder Laurasiatheria (including shrews, moles, and bats) as potential hosts of ancestral hantaviruses at most basal tree nodes. Our data supports the emerging concept of bats as previously overlooked hantavirus reservoir hosts. PMID:27051047

  16. Enveloped virus-like particles as vaccines against pathogenic arboviruses.

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    Pijlman, Gorben P

    2015-05-01

    Arthropod-borne arboviruses form a continuous threat to human and animal health, but few arboviral vaccines are currently available. Advances in expression technology for complex, enveloped virus-like particles (eVLPs) create new opportunities to develop potent vaccines against pathogenic arboviruses. In this short review, I highlight the successes and challenges in eVLP production for members of the three major arbovirus families: Flaviviridae (e.g., dengue, West Nile, Japanese encephalitis); Bunyaviridae (e.g., Rift Valley fever); and Togaviridae (e.g., chikungunya). The results from pre-clinical testing will be discussed as well as specific constraints to the large-scale manufacture and purification of eVLPs, which are complex assemblies of membranes and viral glycoproteins. Insect cells emerge as ideal substrates for correct arboviral glycoprotein folding and posttranslational modification to yield high quality eVLPs. Furthermore, baculovirus expression in insect cell culture is scalable and has a proven safety record in industrial human and veterinary vaccine manufacturing. In conclusion, eVLPs produced in insect cells using modern biotechnology have a realistic potential to be used in novel vaccines against arboviral diseases. PMID:25692281

  17. Thottapalayam virus is genetically distant to the rodent-borne hantaviruses, consistent with its isolation from the Asian house shrew (Suncus murinus

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    Vincent Martin J

    2007-08-01

    Full Text Available Abstract Thottapalayam (TPM virus belongs to the genus Hantavirus, family Bunyaviridae. The genomes of hantaviruses consist of three negative-stranded RNA segments (S, M and L encoding the virus nucleocapsid (N, glycoprotein (Gn, Gc, and polymerase (L proteins, respectively. The genus Hantavirus contains predominantly rodent-borne viruses, with the prominent exception of TPM virus which was isolated in India in 1964 from an insectivore, Suncus murinus, commonly referred to as the Asian house shrew or brown musk shrew. Analysis of the available TPM virus S (1530 nt RNA genome segment sequence and the newly derived M (3621 nt and L (6581 nt segment sequences demonstrate that the entire TPM virus genome is very unique. Remarkably high sequence differences are seen at the nucleotide (up to S – 47%, M – 49%, L – 38% and protein (up to N – 54%, Gn/Gc – 57% and L – 39% levels relative to the rodent-borne hantaviruses, consistent with TPM virus having a unique host association.

  18. Molecular Assay on Crimean Congo Hemorrhagic Fever Virus in Ticks (Ixodidae Collected from Kermanshah Province, Western Iran

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    Maria Mohammadian

    2016-01-01

    Full Text Available Background: Crimean-Congo Hemorrhagic Fever (CCHF is a feverous and hemorrhagic disease endemic in some parts of Iran and caused by an arbovirus related to Bunyaviridae family and Nairovirusgenus. The main virus reser­voir in the nature is ticks, however small vertebrates and a wide range of domestic and wild animals are regarded as reservoir hosts. This study was conducted to determine the infection rate of CCHF virus in hard ticks of Sarpole-Zahab County, Kermanshah province, west of Iran.Methods: From total number of 851 collected ticks from 8 villages, 131 ticks were selected randomlyand investi­gated for detection of CCHF virus using RT-PCR.Results: The virus was found in 3.8% of the tested ticks. Hyalommaanatolicum, H.asiaticum and Rhipicephalus sanguineus species were found to have viral infection, with the highest infection rate (11.11% in Rh. sanguineus.Conclusion: These findings provide epidemiological evidence for planning control strategies of the disease in the study area.

  19. Conserved Endonuclease Function of Hantavirus L Polymerase

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    Sylvia Rothenberger

    2016-05-01

    Full Text Available Hantaviruses are important emerging pathogens belonging to the Bunyaviridae family. Like other segmented negative strand RNA viruses, the RNA-dependent RNA polymerase (RdRp also known as L protein of hantaviruses lacks an intrinsic “capping activity”. Hantaviruses therefore employ a “cap snatching” strategy acquiring short 5′ RNA sequences bearing 5′cap structures by endonucleolytic cleavage from host cell transcripts. The viral endonuclease activity implicated in cap snatching of hantaviruses has been mapped to the N-terminal domain of the L protein. Using a combination of molecular modeling and structure–function analysis we confirm and extend these findings providing evidence for high conservation of the L endonuclease between Old and New World hantaviruses. Recombinant hantavirus L endonuclease showed catalytic activity and a defined cation preference shared by other viral endonucleases. Based on the previously reported remarkably high activity of hantavirus L endonuclease, we established a cell-based assay for the hantavirus endonuclase function. The robustness of the assay and its high-throughput compatible format makes it suitable for small molecule drug screens to identify novel inhibitors of hantavirus endonuclease. Based on the high degree of similarity to RdRp endonucleases, some candidate inhibitors may be broadly active against hantaviruses and other emerging human pathogenic Bunyaviruses.

  20. Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil

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    João Bosco Lima Gimaque

    2012-02-01

    Full Text Available Hantavirus disease is caused by the hantavirus, which is an RNA virus belonging to the family Bunyaviridae. Hantavirus disease is an anthropozoonotic infection transmitted through the inhalation of aerosols from the excreta of hantavirus-infected rodents. In the county of Itacoatiara in the state of Amazonas (AM, Brazil, the first human cases of hantavirus pulmonary and cardiovascular syndrome were described in July 2004. These first cases were followed by two fatal cases, one in the municipality of Maués in 2005 and another in Itacoatiara in 2007. In this study, we investigated the antibody levels to hantavirus in a population of 1,731 individuals from four different counties of AM. Sera were tested by IgG/IgM- enzyme-linked immune-sorbent assay using a recombinant nucleocapsid protein of the Araraquara hantavirus as an antigen. Ten sera were IgG positive to hantavirus (0.6%. Among the positive sera, 0.8% (1/122, 0.4% (1/256, 0.2% (1/556 and 0.9% (7/797 were from Atalaia do Norte, Careiro Castanho, Itacoatiara and Lábrea, respectively. None of the sera in this survey were IgM-positive. Because these counties are distributed in different areas of AM, we can assume that infected individuals are found throughout the entire state, which suggests that hantavirus disease could be a local emerging health problem.

  1. Elevated Cytokines, Thrombin and PAI-1 in Severe HCPS Patients Due to Sin Nombre Virus

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    Virginie Bondu

    2015-02-01

    Full Text Available Sin Nombre Hantavirus (SNV, Bunyaviridae Hantavirus is a Category A pathogen that causes Hantavirus Cardiopulmonary Syndrome (HCPS with case fatality ratios generally ranging from 30% to 50%. HCPS is characterized by vascular leakage due to dysregulation of the endothelial barrier function. The loss of vascular integrity results in non-cardiogenic pulmonary edema, shock, multi-organ failure and death. Using Electric Cell-substrate Impedance Sensing (ECIS measurements, we found that plasma samples drawn from University of New Mexico Hospital patients with serologically-confirmed HCPS, induce loss of cell-cell adhesion in confluent epithelial and endothelial cell monolayers grown in ECIS cultureware. We show that the loss of cell-cell adhesion is sensitive to both thrombin and plasmin inhibitors in mild cases, and to thrombin only inhibition in severe cases, suggesting an increasing prothrombotic state with disease severity. A proteomic profile (2D gel electrophoresis and mass spectrometry of HCPS plasma samples in our cohort revealed robust antifibrinolytic activity among terminal case patients. The prothrombotic activity is highlighted by acute ≥30 to >100 fold increases in active plasminogen activator inhibitor (PAI-1 which, preceded death of the subjects within 48 h. Taken together, this suggests that PAI-1 might be a response to the severe pathology as it is expected to reduce plasmin activity and possibly thrombin activity in the terminal patients.

  2. Phylogeographic analysis of hemorrhagic fever with renal syndrome patients using multiplex PCR-based next generation sequencing.

    Science.gov (United States)

    Kim, Won-Keun; Kim, Jeong-Ah; Song, Dong Hyun; Lee, Daesang; Kim, Yong Chul; Lee, Sook-Young; Lee, Seung-Ho; No, Jin Sun; Kim, Ji Hye; Kho, Jeong Hoon; Gu, Se Hun; Jeong, Seong Tae; Wiley, Michael; Kim, Heung-Chul; Klein, Terry A; Palacios, Gustavo; Song, Jin-Won

    2016-01-01

    Emerging and re-emerging infectious diseases caused by RNA viruses pose a critical public health threat. Next generation sequencing (NGS) is a powerful technology to define genomic sequences of the viruses. Of particular interest is the use of whole genome sequencing (WGS) to perform phylogeographic analysis, that allows the detection and tracking of the emergence of viral infections. Hantaviruses, Bunyaviridae, cause hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) in humans. We propose to use WGS for the phylogeographic analysis of human hantavirus infections. A novel multiplex PCR-based NGS was developed to gather whole genome sequences of Hantaan virus (HTNV) from HFRS patients and rodent hosts in endemic areas. The obtained genomes were described for the spatial and temporal links between cases and their sources. Phylogenetic analyses demonstrated geographic clustering of HTNV strains from clinical specimens with the HTNV strains circulating in rodents, suggesting the most likely site and time of infection. Recombination analysis demonstrated a genome organization compatible with recombination of the HTNV S segment. The multiplex PCR-based NGS is useful and robust to acquire viral genomic sequences and may provide important ways to define the phylogeographical association and molecular evolution of hantaviruses. PMID:27221218

  3. Segment-specific terminal sequences of Bunyamwera bunyavirus regulate genome replication

    International Nuclear Information System (INIS)

    Bunyamwera virus (BUNV) is the prototype of both the Orthobunyavirus genus and the Bunyaviridae family of segmented negative sense RNA viruses. The tripartite BUNV genome consists of small (S), medium (M), and large (L) segments that are transcribed to give a single mRNA and replicated to generate an antigenome that is the template for synthesis of further genomic RNA strands. We modified an existing cDNA-derived RNA synthesis system to allow identification of BUNV RNA replication and transcription products by direct metabolic labeling. Direct RNA analysis allowed us to distinguish between template activities that affected either RNA replication or mRNA transcription, an ability that was not possible using previous reporter gene expression assays. We generated genome analogs containing the entire nontranslated terminal sequences of the S, M, and L BUNV segments surrounding a common sequence. Analysis of RNAs synthesized from these templates revealed that the relative abilities of BUNV segments to perform RNA replication was M > L > S. Exchange of segment-specific terminal nucleotides identified a 12-nt region located within both the 3' and 5' termini of the M segment that correlated with its high replication ability

  4. Conserved Endonuclease Function of Hantavirus L Polymerase.

    Science.gov (United States)

    Rothenberger, Sylvia; Torriani, Giulia; Johansson, Maria U; Kunz, Stefan; Engler, Olivier

    2016-01-01

    Hantaviruses are important emerging pathogens belonging to the Bunyaviridae family. Like other segmented negative strand RNA viruses, the RNA-dependent RNA polymerase (RdRp) also known as L protein of hantaviruses lacks an intrinsic "capping activity". Hantaviruses therefore employ a "cap snatching" strategy acquiring short 5' RNA sequences bearing 5'cap structures by endonucleolytic cleavage from host cell transcripts. The viral endonuclease activity implicated in cap snatching of hantaviruses has been mapped to the N-terminal domain of the L protein. Using a combination of molecular modeling and structure-function analysis we confirm and extend these findings providing evidence for high conservation of the L endonuclease between Old and New World hantaviruses. Recombinant hantavirus L endonuclease showed catalytic activity and a defined cation preference shared by other viral endonucleases. Based on the previously reported remarkably high activity of hantavirus L endonuclease, we established a cell-based assay for the hantavirus endonuclase function. The robustness of the assay and its high-throughput compatible format makes it suitable for small molecule drug screens to identify novel inhibitors of hantavirus endonuclease. Based on the high degree of similarity to RdRp endonucleases, some candidate inhibitors may be broadly active against hantaviruses and other emerging human pathogenic Bunyaviruses. PMID:27144576

  5. Directory of Open Access Journals (Sweden)

    Ioni Oliveira Santos

    2013-01-01

    Full Text Available INTRODUCTION: Hantavirus is a genus of ribonucleic acid (RNA viruses included in the family Bunyaviridae. Hantaviruses are rodent-borne zoonoses that, in the last 18 years, became an emergent public health problem in the Americas, causing a severe cardiopulmonary syndrome. This disease has no specific treatment and has a high case fatality. The transmission of hantavirus to man occurs by inhaling aerosols of rodent excreta. The aim of this study was to determine the prevalence of antibodies to hantavirus in the population of the rural settlement of Tupã in the county of Marcelândia, state of Mato Grosso, Brazil. METHODS: The participants of the serologic survey were visited at their homes and selected randomly among the settlement population. Blood samples of the participants were collected by venopuncture. The serum samples were tested by an IgG-ELISA using an N recombinant protein of Araraquara hantavirus as antigen, using the protocol previously established by Figueiredo et al. RESULTS: IgG antibodies to hantavirus were detected in 7 (13% of the 54 participants. The positivity was higher among men. It was observed that there was an association of seropositivity to hantavirus within the participants born in the south of Brazil. CONCLUSIONS: The results suggest that, in this rural area, everyone is exposed to the same risk of becoming infected with hantavirus, and, therefore, there is a need to intensify surveillance activities and education of the local people to prevent this viral infection.

  6. Ultrastructural, Antigenic and Physicochemical Characterization of the Mojuí dos Campos (Bunyavirus Isolated from Bat in the Brazilian Amazon Region

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    Wanzeller Ana LM

    2002-01-01

    Full Text Available The Mojuí dos Campos virus (MDCV was isolated from the blood of an unidentified bat (Chiroptera captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i, and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.

  7. Enzootic Arbovirus Surveillance in Forest Habitat and Phylogenetic Characterization of Novel Isolates of Gamboa Virus in Panama.

    Science.gov (United States)

    Eastwood, Gillian; Loaiza, Jose R; Pongsiri, Montira J; Sanjur, Oris I; Pecor, James E; Auguste, Albert J; Kramer, Laura D

    2016-04-01

    Landscape changes occurring in Panama, a country whose geographic location and climate have historically supported arbovirus transmission, prompted the hypothesis that arbovirus prevalence increases with degradation of tropical forest habitats. Investigations at four variably degraded sites revealed a diverse array of potential mosquito vectors, several of which are known vectors of arbovirus pathogens. Overall, 675 pools consisting of 25,787 mosquitoes and representing 29 species from nine genera (collected at ground and canopy height across all habitats) were screened for cytopathic viruses on Vero cells. We detected four isolates of Gamboa virus (family:Bunyaviridae; genus:Orthobunyavirus) from pools ofAedeomyia squamipenniscaptured at canopy level in November 2012. Phylogenetic characterization of complete genome sequences shows the new isolates to be closely related to each other with strong evidence of reassortment among the M segment of Panamanian Gamboa isolates and several other viruses of this group. At the site yielding viruses, Soberanía National Park in central Panama, 18 mosquito species were identified, and the predominant taxa includedA. squamipennis,Coquillettidia nigricans, andMansonia titillans. PMID:26834200

  8. Genetic and Phylogenetic Characterization of Tataguine and Witwatersrand Viruses and Other Orthobunyaviruses of the Anopheles A, Capim, Guamá, Koongol, Mapputta, Tete, and Turlock Serogroups.

    Science.gov (United States)

    Shchetinin, Alexey M; Lvov, Dmitry K; Deriabin, Petr G; Botikov, Andrey G; Gitelman, Asya K; Kuhn, Jens H; Alkhovsky, Sergey V

    2015-11-01

    The family Bunyaviridae has more than 530 members that are distributed among five genera or remain to be classified. The genus Orthobunyavirus is the most diverse bunyaviral genus with more than 220 viruses that have been assigned to more than 18 serogroups based on serological cross-reactions and limited molecular-biological characterization. Sequence information for all three orthobunyaviral genome segments is only available for viruses belonging to the Bunyamwera, Bwamba/Pongola, California encephalitis, Gamboa, Group C, Mapputta, Nyando, and Simbu serogroups. Here we present coding-complete sequences for all three genome segments of 15 orthobunyaviruses belonging to the Anopheles A, Capim, Guamá, Kongool, Tete, and Turlock serogroups, and of two unclassified bunyaviruses previously not known to be orthobunyaviruses (Tataguine and Witwatersrand viruses). Using those sequence data, we established the most comprehensive phylogeny of the Orthobunyavirus genus to date, now covering 15 serogroups. Our results emphasize the high genetic diversity of orthobunyaviruses and reveal that the presence of the small nonstructural protein (NSs)-encoding open reading frame is not as common in orthobunyavirus genomes as previously thought. PMID:26610546

  9. Simultaneous Detection of Three Arboviruses Using a Triplex RT-PCR Enzyme Hybridization Assay

    Institute of Scientific and Technical Information of China (English)

    Dan Dong; Shi-hong Fu; Li-hua Wang; Zhi Lv; Tai-yuan Li; Guo-dong Liang

    2012-01-01

    Arboviruses represent a serious problem to public health and agriculture worldwide.Fast,accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation.We developed a cost-effective,rapid,and highly sensitive one-step "triplex RT-PCR enzyme hybridization"assay for simultaneous detections of Japanese Encephallitis virus (JEV,Flaviviridae)Getah virus (GETV,Togaviridae),and Tahyna virus (TAHV,Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction.The analytical sensitivity of this assay was 1 PFU/mL for JEV,10PFU/mL for GETV,and 10 PFU/mL for TAHV.This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods.When “triplex RT-PCR enzyme hybridization” was applied to 29 cerebrospinal fluid(CSF)samples that were JEV-positive by normal RT-PCR assay,all samples were strongly positive for JEV,but negative for GETV and TAHV,demonstrating a good sensitivity,specificity,and performance at CSF specimen detection.

  10. Oficina de autoexame de mamas: uma estratégia para o autoconhecimento de adolescentes Taller de autoexamen de mamas: una estrategia para el autoconocimiento de adolescentes Workshop on breast self-examination: a strategy for self-knowledge of adolescents

    Directory of Open Access Journals (Sweden)

    Maria da Conceição Grego

    2011-01-01

    Full Text Available OBJETIVOS: Verificar a repercussão do conhecimento transmitido por meio da Oficina de autoexame de mamas e identificar a multiplicação de informações pelas participantes adolescentes. MÉTODOS: Trata-se de um estudo quantitativo, tipo Survey, realizado com 474 adolescentes de três escolas de Ensino Médio e Fundamental do município de Embu das Artes, em 2006 e 2007. RESULTADOS: Os dados das questões estruturadas e semiestruturadas, respondidas pelas participantes, mostraram as frequências das respostas acima de 50% da estimativa sobre a "repercussão do conteúdo da oficina" e abaixo dos 50% sobre a "ação multiplicadora". CONCLUSÃO: A Oficina auxilia a elaboração do conceito de si pelas adolescentes, de seu corpo, informa sobre o câncer de mama e os benefícios da adoção de práticas e atitudes saudáveis em seu cotidiano, empregando a técnica do autoexame de mamas como instrumento pedagógico.OBJETIVOS: Verificar la repercusión del conocimiento transmitido por medio del Taller de autoexamen de mamas e identificar la multiplicación de las informaciones por las participantes adolescentes. MÉTODOS: Se trata de un estudio cuantitativo, tipo Survey, realizado con 474 adolescentes de tres escuelas de Enseñanza Media y Fundamental del municipio de Embu de las Artes, en el 2006 y 2007. RESULTADOS: Los datos de las preguntas estructuradas y semiestructuradas, respondidas por las participantes, mostraron las frecuencias de las respuestas encima del 50% de la estimativa sobre la "repercusión del contenido del Taller" y abajo del 50% sobre la "acción multiplicadora". CONCLUSIÓN: El taller auxilia en la elaboración del concepto de sí por parte de las adolescentes, de su cuerpo, informa sobre el cáncer de mama y los beneficios de la adopción de prácticas y actitudes saludables en su cotidiano, empleando la técnica del autoexamen de mamas como instrumento pedagógico.OBJECTIVES: To verify the repercussions of knowledge

  11. Spontaneous morphogenetic juvenilization observed in laboratory populations of vector species of Chagas disease (Triatominae

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    Alina Perlowagora-Szumlewicz

    1973-08-01

    èscoordenado manifestado por formas adultas grotescas, 5 por adultos supernumerários incapazes de completar sua metamorfose e 6 por adultos supernumerários completos, produzidos por ninfas de 6º estágio. A remetida ocorrência de insetos com idênticos graus de juvenilização, dentro de uma população, é uma indicação de que existe nestes casos, uma aberração hereditária. Os fatores responsáveis pela juvenilização morfogenética não podem ser transmitidos através de insetos juvenilizados, porque esses são estéreis, consequentemente foram transmitidos através de insetos normais, provavelmente como um recessivo ou um grupo de fatores recessivos. A juvenilização morfogenética espontânea, observada em populações de laboratório, tem notável similaridade com os efeitos juvenilizantes induzidos pela aplicação de hormônios juvenis análogos, descritos na literatura e também obtidos no nosso laboratório num estudo em vias de publicação. Desse modo, admite-se que, não só as alterações fenotipicas que ocorrem nas populações naturais, mas também as suas "fenocópias" induzidas pela aplicação de hormônios juvenis, sejam fenômenos produzidos por reações idênticas, geneticamente controladas.

  12. Transmissibilidade e efeito de Acremonium strictum em sementes de milho Transmissibility and effect of Acremonium strictum in maize seeds

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    Hudson Teixeira

    2003-10-01

    Full Text Available Acremonium strictum é patogênico ao milho e a outras culturas, podendo causar podridão de sementes, podridão de colmos e a morte de plântulas. Trata-se de um fungo que pode interferir no vigor, estande e produtividade final das plantas. Além disso, pode ser disseminado e transmitido à progênie via sementes, ainda que não sejam conhecidas as proporções reais em que isso ocorre. Neste trabalho, avaliou-se o efeito de A. strictum nas qualidades fisiológica e sanitária de sementes e plântulas, no crescimento vegetativo e na sanidade (taxas de infecção e de transmissão de plantas de milho em seu estádio inicial de desenvolvimento. As taxas de infecção e de transmissão do fungo foram avaliadas usando-se a técnica da restrição hídrica, previamente desenvolvida para infectar as sementes. O meio MA (extrato de malte-ágar, suplementado com manitol para obter o potencial de água de -1,4 MPa, foi o substrato utilizado na inoculação das sementes. Observou-se que a germinação das sementes de milho foi reduzida por A. strictum em períodos de contato com a colônia fúngica acima de 72 horas. O vigor e a viabilidade potenciais das sementes de milho não foram afetados pela presença de A. strictum. Após 28 dias da semeadura, a incidência de A. strictum reduziu o crescimento vegetativo de plantas de milho. O fungo A. strictum, oriundo de sementes inoculadas, foi transmitido às plantas jovens de milho.Acremonium strictum is pathogenic to seeds, stalk and seedlings of maize. It is a fungus that can interfere in the vigor, stand and final productivity of plants. In addition, it can be disseminated and transmitted through seeds to the progeny, although the real proportions of that are not well known. This study reports the effects of A. strictum on maize seeds and seedlings quality (germination, vigor, healthness, some vegetative growth parameters and also sanity (infection and transmission rates of young maize plants. The

  13. Genética de Coffea. XVIII - Variegação no cafeeiro Leaf variegation in coffee plants

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    A. Carvalho

    1954-01-01

    Full Text Available A variegação das fôlhas do cafeeiro tanto em plantas novas como em plantas adultas tem sido freqüentemente observada. Plantas variegadas podem ocorrer espontaneamente nas progénies das diversas variedades de Coffea arabica L., motivo porque não devem constituir variedade distinta dessa espécie, como foi proposto por Cramer. As plantas variegadas em estudo puderam ser agrupadas em duas categorias, isto é, aquelas em que a variegação não se acha associada com anomalias na forma e textura das folhas e aquelas nas quais a variegação é acompanhada dessas anomalias. No primeiro grupo o padrão de variegação não é uniforme para tôdas as fôlhas, enquanto no segundo grupo o padrão é semelhante para tôda a planta. Usualmente apenas alguns ramos das plantas do primeiro grupo são variegados. Observou-se algumas vezes que as duas fôlhas do mesmo verticilo têm padrões semelhantes de variegação. Encontraram-se também fôlhas variegadas nas quais a metade da lâmina é variegada ou mesmo albina, enquanto a outra metade apresenta a côr verde normal. Os dados genéticos obtidos, embora preliminares para a maioria das plantas variegadas em investigação, permitiram concluir que o padrão de variegação da planta 180, pertencente ao primeiro grupo, é herdado pelo citoplasma e não é transmitido pelo pólen. O padrão de variegação da planta 253-21, pertencente ao segundo grupo, no entanto, parece ser transmitido pelo polen.Leaf variegation in coffee plants is a relatively common abnormality, and variegated seedlings have been found to occur spontaneously in progenies of most varieties of Coffea arabica L. For this reason it is thought that variegated types should not be described as distinct varieties, as it was done before by Cramer. Variegated coffee plants can be grouped in two categories : first, those in which variegated leaves are normal in shape and texture, but do not show a uniform variegation pattern; and second, those

  14. Entremeando Saúde e Doença: A Condição de Soropositividade para o HIV no Contexto de Culto à Vida Saudável

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    Camila Miranda de Amorim Resende

    2013-01-01

    Full Text Available A través del trabajo de análisis del discurso de cinco adolescentes infectados con el VIH por transmisión vertical tratados en una unidad de salud en una ciudad del Rio de Janeiro, cuestionamos el ideal contemporáneo de vivir una vida saludable. Tal condición apunta a un proceso de subjetivación demasiado complejo, cuando, por un lado, el virus del VIH si transmitido por los padres no puede, en este caso, considerarse como consecuencia de una elección individual en cuanto a comportamientos de riesgo - como suele ser asignado la mayoría de los casos de transmisión del VIH. Por otro lado, requiere del sujeto el trabajo de un vivir saludable demasiado difícil, ya que requiere el cuidado permanente de su propia salud y recomendaciones para con la salud de los demás con el fin de no transmitir el virus del VIH. Teniendo en cuenta esa necesidad de gestión de riesgo potencializada, las personas en esta condición de seropositividad se encuentran marcadas por un dispositivo de normalización intenso, sometidas como lo son, desde su nacimiento, a las reglas del buen vivir de acuerdo a las recetas de la prevención. Finalmente, pensamos que la peculiaridad de la condición de seropositividad para el VIH por transmisión vertical se abre a la posibilidad de nos preguntarnos cuanto a lo que significa hoy la oposición entre salud y enfermedad que, en este caso, requiere un estatuto "entre", tejiendo salud y enfermedad.

  15. Broadcast yourself: ¿oxímoron o confusión entre medios? Broadcast Yourself: Oxymoron or Media Mistake?

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    José Luis Fernández

    2009-10-01

    Full Text Available En este trabajo se analiza el hecho de que Internet ofrezca la posibilidad de seleccionar los contenidos que construirían radios individualizadas. Cada usuario de la Red podría ahora seleccionar contenidos radiofónicos reemplazando a las emisoras de radio tradicionales que siempre han transmitido en modelo «broadcasting». Se exponen resultados previos de nuestras investigaciones que permiten comparar la radio todavía existente con algunas de las ofertas presentes en la Red. La comparación permite en las conclusiones establecer la presencia de dos procedimientos de escucha de radio en la Red: el habitual, que permite la coexistencia entre escucha y práctica social paralela (trabajo, conducción de automóviles, etc. y el propio de Internet, frente en la web de la emisora, que obliga a la atención visual además de la sonora. Resulta inevitable considerar a este último como una pérdida, frente la independencia visual que la radio siempre ha permitido. This article analyses the possibility offered by the Internet to select radio content to build customized radios. Each internet user could select radio content, replacing the traditional radio stations that have always broadcasted. Previous results from our investigations are presented to allow us to compare the still-existing traditional radio to some of the offers on the Internet. Such a comparison will allow us to determine two different ways of listening to the radio on the Internet: the usual one, which allows the coexistence of listening and any parallel social practice (work, driving, etc., and another one, appropriate to the internet, that adds visual attention to hearing. It is unavoidable to consider this as a loss compared to the visual independence that radio has always allowed.

  16. Diagnóstico de la ehrlichiosis monocítica canina: una revisión actualizada

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    Roger Iván Rodríguez-Vivas

    2015-01-01

    Full Text Available La ehrlichiosis monocítica canina (EMC es causada por la infección de una rickettsia denominada Ehrlichia canis; este microorganismo se reproduce solamente en el citoplasma de los monocitos sanguíneos y macrófagos tisulares. La EMC ha sido reportada en todo el mundo, pero las frecuencias más altas se reportan en las regiones tropicales y subtropicales. La EMC no siempre es reconocida clínicamente, pues es difícil de diagnosticar debido a que los signos clínicos son variables, a que se presenta en diferentes fases y a la existencia de coinfecciones con otros patógenos transmitidos por garrapatas y otros agentes infecciosos. Las manifestaciones clínicas y las técnicas de diagnóstico tradicionales, como el diagnóstico hematológico y la demostración típica de mórulas de E. canis en el citoplasma de monocitos en frotis sanguíneos, se han utilizado para el diagnóstico de la EMC; sin embargo, las pruebas de diagnóstico por PCR y la serología mediante el uso de antígenos específicos son las pruebas más sensibles y específicas para el diagnóstico de la enfermedad. Este artículo presenta una revisión actualizada sobre el diagnóstico de la infección por E. canis en perros.

  17. Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e mosaico amarelo do feijoeiro Electron microscopy of common and yellow bean mosaic viruses in infected tissues

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    I. J. B. de Camargo

    1968-01-01

    Full Text Available Exames ao microscópio electrônico de tecidos foliares e radiculares de plantas infetadas pelo vírus do mosaico comum ou do mosaico amarelo do feijoeiro, mostraram a presença de dois tipos de inclusões no cito-plasma: filamentosas, consideradas como partículas de vírus, e lamelares, típicas dos vírus do grupo Y. Essas inclusões não foram encontradas no pólen ou no óvulo de feijoeiros infetados. Como o vírus do mosaico comum do feijoeiro é transmitido pelo pólen, sugere-se que êle ocorre nestas células em concentração muito baixa, ou mesmo na forma de ácido nucléico.Two types of cytoplasmic inclusions were observed in leaf and root tissues of host plants infected with the common and yellow bean mosaic viruses: (1 filamentous inclusions considered as an aggregate of virus particles and (2 lamellar inclusions which appeared with varied configurations that represent sections at different angles of the same cylindrical structure. No type of inclusion or virus particle was seen in pollen and ovule from bean plants infected with each of the two viruses. Since, however, the common bean mosaic virus is transmitted through the pollen it is suggested that it occurs in very low concentration in this structure or else as viral nucleic acid.

  18. The politics of the self: psychological science and bourgeois subjectivity in 19th century Spain

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    Novella, Enric J.

    2010-12-01

    Full Text Available This paper offers an analysis of the process of institutionalization of psychological knowledge in Spain following the educative reforms implemented during the second third of the 19th century, which prescribed its inclusion in the curricular program of the new secondary education. After a detailed examination of the theoretical orientation, the ideological assumptions and the sociopolitical connections of the contents transmitted to the students throughout the century, its militant spiritualism is interpreted as a highly significant attempt on the part of the liberal elites to articulate a pedagogy of subjectivity intended to counteract the trends toward reduction, naturalization and fragmentation of psychic life inherent to the development of modern science.

    En este artículo se ofrece un análisis del proceso de institucionalización del conocimiento psicológico en España por obra de las reformas educativas implementadas durante el segundo tercio del siglo XIX, que prescribieron su inclusión en el programa curricular de la nueva educación secundaria. Tras un examen detenido de la orientación doctrinal, los supuestos ideológicos y la filiación sociopolítica de los contenidos transmitidos a los alumnos durante la mayor parte de la centuria, se interpreta su espiritualismo militante como un intento muy significativo por parte de las élites liberales de articular una pedagogía de la subjetividad destinada a contrarrestar las tendencias de reducción, naturalización y fragmentación del psiquismo alentadas por el desarrollo de la ciencia moderna.

  19. Dengue en el Perú: a un cuarto de siglo de su reemergencia

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    César Cabezas

    2015-01-01

    Full Text Available Un problema sanitario cada vez más frecuente y disperso en áreas tropicales y subtropicales del mundo, incluido el Perú donde ingresó en 1990, es el dengue. Es producido por el virus del dengue con cuatro serotipos y transmitido por el Aedes aegypti, vector que convive con los humanos y cuya presencia es favorecida por deficientes condiciones sanitarias, sociales y económicas. Manifestaciones de formas severas de la enfermedad como el choque y sangrado, están relacionadas con la frecuente cocirculación de los cuatro serotipos y la aparición de nuevos genotipos como el americano/asiático del serotipo 2. La nueva clasificación de la enfermedad por la OMS como dengue con o sin signos de alarma y dengue grave, está contribuyendo a un diagnóstico y tratamiento más oportunos, permitiendo reducir la letalidad. Debe destacarse la necesidad de la vigilancia del síndrome febril y los índices aédicos que contribuyan a un diagnóstico oportuno y orienten las medidas de control vectorial mediante educación sanitaria y manejo ambiental con participación comunitaria e intersectorial, de manera creativa según los nichos ecológicos. Una alternativa de prevención complementaria sería la vacunación utilizando vacunas tetravalentes cuya seguridad y eficacia deben estar garantizadas antes de su uso poblacional en el marco de estrategias integrales.

  20. Dengue en el Perú: a un cuarto de siglo de su reemergencia

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    César Cabezas

    2015-03-01

    Full Text Available Un problema sanitario cada vez más frecuente y disperso en áreas tropicales y subtropicales del mundo, incluido el Perú donde ingresó en 1990, es el dengue. Es producido por el virus del dengue con cuatro serotipos y transmitido por el Aedes aegypti, vector que convive con los humanos y cuya presencia es favorecida por deficientes condiciones sanitarias, sociales y económicas. Manifestaciones de formas severas de la enfermedad como el choque y sangrado, están relacionadas con la frecuente cocirculación de los cuatro serotipos y la aparición de nuevos genotipos como el americano/asiático del serotipo 2. La nueva clasificación de la enfermedad por la OMS como dengue con o sin signos de alarma y dengue grave, está contribuyendo a un diagnóstico y tratamiento más oportunos, permitiendo reducir la letalidad. Debe destacarse la necesidad de la vigilancia del síndrome febril y los índices aédicos que contribuyan a un diagnóstico oportuno y orienten las medidas de control vectorial mediante educación sanitaria y manejo ambiental con participación comunitaria e intersectorial, de manera creativa según los nichos ecológicos. Una alternativa de prevención complementaria sería la vacunación utilizando vacunas tetravalentes cuya seguridad y eficacia deben estar garantizadas antes de su uso poblacional en el marco de estrategias integrales.

  1. Maneiras de fazer, modos de proceder: a tradição reinventada do pão de canela na Serra da Mantiqueira, Minas Gerais

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    Rogéria Campos de Almeida Dutra

    2012-12-01

    Full Text Available Este artigo traz como proposta a investigação do processo de produção do pão de canela na Serra do Ibitipoca, no sudeste de Minas Gerais, através da qual se procura abordar as transformações dos saberes transmitidos entre gerações e seus novos sentidos ante as dinâmicas contemporâneas. Como parte da tradição culinária local, sua fabricação envolve modos de saber e fazer que frente ao desenvolvimento turístico são simultaneamente reafirmados e reinventados. Sua elaboração, ao abrigar segredos culinários, sistemas gestuais e combinações de sabores, expressam a matriz das formas culturais configuradas pelas disposições práticas, cenário da cultura tal como vivida, que envolvem, a partir de uma tradição oralmente transmitida, a operação de uma criatividade prática fundamentada em atos da vida cotidiana.This paper aims at investigating the production of pão de canela at the Serra do Ibitipoca region situated in the southeast of Minas Gerais, Brazil. As part of the local culinary tradition, its manufacture involves ways of knowing and doing that are reaffirmed and reinvented by tourism development. Culinary secrets, gestural systems and flavors' combinations present in this kind of bread express culture as it is lived, an orally transmitted activity supported by a practical creativity of daily life.

  2. Assimetria de transmissão de preço na comercialização da uva fina de mesa no Paraná: 1997 a 2011

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    Alexandre Florindo Alves

    2013-09-01

    Full Text Available O Paraná se destaca como um dos principais produtores de uva fina de mesa no Brasil, tendo como característica a presença de pequenos e médios produtores. O presente estudo objetiva analisar a assimetria na transmissão de preço entre os níveis produtor, atacado e varejo da uva fina de mesa no Paraná, no período de janeiro de 1997 a outubro de 2011. A metodologia empregada para verificar como ocorre a transmissão de preços entre os agentes foi o Vetor Autorregressivo (VAR. O modelo utilizado para mensurar Assimetria de Transmissão de Preços (ATP foi baseado na metodologia desenvolvida por Grififth e Piggott (1994. Os principais resultados foram: na análise de transmissão de preço ao varejo, o atacado apresentou um coeficiente de elasticidade maior do que o nível do produtor; o sentido unicausal atacado-varejo do teste de Granger é uma evidência de presença de informações assimétricas; e os acréscimos dos preços são transmitidos com mais rapidez do que os decréscimos, que, junto aos resultados do teste t de Griffith e Piggott (1994, comprova a existência de assimetria na transmissão de preços.

  3. Televisión, clima familiar y percepción de valores en adolescentes con TDA-H y adolescentes estándar

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    C Medrano Samaniego

    2013-03-01

    Full Text Available La cultura multimedia en la que vivimos ofrece pocas oportunidades para entrenar la atención y puede dificultar la construcción del sentido de los mensajes transmitidos, por lo que, en esta investigación el objetivo general fue determinar las semejanzas y diferencias entre adolescentes con TDAH y estándar en el perfil de consumo televisivo en relación con el clima familiar y los valores percibidos. La muestra total es de 209 adolescentes con TDAH y estándar con edades comprendidas entre los 14 y 18 años. Se utilizaron los cuestionarios CH-TV 0.2 y VAL.TV.02. Los resultados indican que, en general, los adolescentes se concentran en dos perfiles televisivos mayoritarios diferenciados por el grado de elección de géneros televisivos y la relevancia otorgada al atractivo físico del personaje relacionado con los valores de apertura al cambio y autopromoción, junto a la cohesión y expresividad familiar. Sin embargo, existen diferencias destacables entre el grupo con TDAH y estándar respecto a dichos perfiles. Estos datos tienen su interés por contribuir al estudio de las diferencias individuales en relación con el consumo mediático, y por sentar las bases para la elaboración de programas de competencia mediática que se ajusten a las características de los colectivos a los que van dirigidos.

  4. DESCRIPCIÓN MODULAR DE UN ESQUEMA DE CODIFICACIÓN CONCATENADO PARA CORRECCIÓN DE ERRORES CON PROGRAMACIÓN DE HARDWARE MODULAR DESIGN OF SCHEME CODING CONCATENATED FOR CORRECTION ERROR WITH PROGRAMMING OF HARDWARE

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    Cecilia E Sandoval Ruiz

    2008-09-01

    Full Text Available Las comunicaciones inalámbricas requieren el empleo de métodos de corrección de errores sobre los datos transmitidos, usándose generalmente técnicas de codificación Reed-Solomon & Viterbi, por razones de desempeño y seguridad es preferible implementarlos sobre hardware. En este trabajo se presenta el diseño modular de la etapa de codificación de éstos códigos para su concatenación usando VHDL (VHSIC Hardware Descriptor Language, orientado a la implementación sobre tecnología de matriz de compuertas programadas por campo (FPGA, Se inicia con una revisión de los conceptos asociados a la definición de los componentes, y el modelo, descripción del comportamiento, luego la arquitectura es diseñada usando la sintaxis en VHDL y es capturado el diseño de hardware, finalmente se presentan los resultados de síntesis.The wireless communication medium require employing forward error correction methods on the data transferred, where Reed-Solomon & Viterbi coding techniques are utilized, because of performance and security reaso. In this paper we present a modular design of phase encoding these codes for concatenation using VHDL (VHSIC Hardware Descriptor Language and oriented to implementation with field programmable gate arrays (FPGA. The work besing with a review of code concept and the definition of the components and the model and the description of the behavioral. Later, the architecture is designed and captures using syntax in VHDL, and finally presents the results of synthesis.

  5. Transmissible venereal tumor in the palpebral conjunctiva of a dog: case reportTumor venéreo transmissível na conjuntiva palpebral de um cão – relato de caso

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    Luciane de Albuquerque

    2011-08-01

    Full Text Available The transmissible venereal tumor (TVT is a contagious neoplasm that occurs in sexually mature dogs, usually transmitted by coitus. This tumor normally affects the genital mucosa and is rarely found in any other part of the body. A case of transmissible venereal tumor in the palpebral conjunctiva of a 3-year old, crossbreed, male dog with a history of an abnormal mass in the right eye was presented. Ophthalmic examination revealed a mass originated from the lower eyelid conjunctiva of the right eye. No other ocular abnormalities were detected. Cytological examination was carried out and the diagnosis was TVT. The dog was treated with lyophilized vincristine sulphate intravenously, once a week, for four weeks. Despite the atypical clinical presentation, the response to chemotherapy with vincristine was excellent leading to complete regression of the neoplasm and no relapse after a year.O tumor venéreo transmissível (TVT é uma neoplasia contagiosa que ocorre em cães sexualmente maduros, sendo geralmente transmitido pelo coito. A neoplasia normalmente afeta a mucosa genital e é raramente encontrada em outras partes do corpo. Apresentamos um caso de tumor venéreo transmissível na conjuntiva palpebral de um cão macho, sem raça definida, com três anos de idade e histórico de presença de uma massa na conjuntiva palpebral inferior do olho direito. Não foram detectadas outras alterações oculares. O diagnóstico citológico da massa foi TVT. O cão foi tratado com sulfato de vincristina por via intravenosa, uma vez por semana, durante quatro semanas. Embora a apresentação do caso seja atípica, a resposta à quimioterapia foi excelente, levando a regressão completa da neoplasia, sem recorrência após um ano.

  6. Identificación de virus que afectan el Loroco (Fernaldia pundurata en el Valle de Zapotitán, El Salvador

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    Reina Flor Guzm\\u00E1n de Serrano

    2006-01-01

    Full Text Available El loroco (Fernaldia pandurata es una especie hortícola de gran valor comercial y consumo en la dieta del pueblo salvadoreño y guatemalteco. Desafortunadamente, esta especie es afectada por diversas plagas entre las que se encuentran la mosca blanca Bemisia tabaci, áfidos y enfermedades de aparente naturaleza viral. En esta investigación se examinaron plantas de loroco con síntomas de mosaico y deformación foliar e inflorescencias cloróticas con el fin de determinar su etiología y posibles agentes vectores. Las muestras se examinaron mediante microscopía electrónica y serología. La observación de las muestras en el microscopio electrónico reveló la presencia de partículas filamentosas (600-700 nm e isométricas (30 nm de aparente naturaleza viral. Las pruebas serológicas demostraron que el virus filamentoso es una especie del género Potyvirus, y el virus isométrico es una especie del género Cucumovirus. No se encontraron virus del género Begomovirus transmitidos por mosca blanca. Se concluye aquí que este insecto actúa solamente como plaga directa del loroco, mientras que los áfidos se comportan como plaga y posibles vectores de los virus detectados. Este es el primer informe sobre patógenos virales del loroco

  7. Desenvolvendo tecnologia para prevenção e tratamento de emergências domésticas para cegos

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    Lorita Marlena Freitag Pagliuca

    1996-03-01

    Full Text Available O aprimoramento do desenvolvimento dos órgãos dos sentidos se dá pela percepção de estímulos do meio, o que leva o homem à apreensão do conhecimento. A pessoa privada da visão conta com seus sentidos remanescentes para sua relação com o que a rodeia. O cego está sujeito a emergências domésticas, comuns a todas as pessoas, mas tem dificuldades de acesso a processos educativos que abordem a prevenção e tratamento destas emergências. Em um Curso de Prevenção e Tratamento de Emergências Domésticas para Cegos, desenvolveu-se tecnologia pedagógica abordando anatomia e fisiologia dos sistemas esquelético, circulatório e pulmonar, além da prevenção e tratamento para desobstrução das vias aéreas, parada cardio-pulmonar, queimaduras, choque elétrico, traumas, hemorragias, envenenamentos, desmaios, convulsões e uso de medicação oral. Utilizou-se esqueleto humano, manequim, moldes, papéis, tubos de plástico e borracha e, principalmente, comunicação verbal e táctil. Os conteúdos transmitidos foram aplicados em situações simuladas. A tecnologia mostrou-se eficaz pela capacidade demonstrada pelos cegos através da verbalização e repetição dos procedimentos.

  8. Parasitosis intestinales en relación con la cultura higiénica de poblaciones rurales de Catamarca.

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    Monferrán, M. C.

    2003-01-01

    Full Text Available Los estudios realizados sobre prevalencia de parasitosis intestinales humanas en comunidades urbanas y rurales en la provincia de Catamarca, revela altos índices. Las causas son múltiples, entre las que se encuentran factores ecológicos y sociales. Las condiciones climáticas imperantes en una zona geográfica resultaran favorables o no a la presentación de algunas parasitosis como por ejemplo las geohelmintiasis.Se atribuye igual importancia a la educación, vivienda, alimentación, recursos económicos, falta de higiene, contaminación ambiental, etc.. Se realizan estudios de prevalencia de parasitosis intestinales en pobladores de dos regiones climáticamente diferentes de nuestra provincia como son los Dptos. La Paz y Paclín detectándose en algunas comunidades índices altos de hasta el 81 %.También se ha notado la influencia climática regional sobre la distribución de las parasitosis intestinales .En La Paz, existe un predominio de las protozoosis y en Paclín las geohelmintiasis son frecuentes y diversas. Las parasitosis detectadas estarían relacionadas en un alto grado a la denominada cultura higiénica, pues las prácticas de la higiene individual y familiar, la deposición de sus excretas, la convivencia con animales domésticos, el manejo de sus rebaños, son costumbres regionales que se han transmitido por generaciones e influyen notablemente en la difusión y la conservación de estos altos índices de parasitosis intestinales en detrimento de la salud de los habitantes.

  9. Entrevista con Georges Duby.

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    - Consejo de Redacción

    1994-01-01

    Full Text Available Duby, heredero de dos grandes historiadores como Marc Bloch y Lucien Febvre, es uno de los más importantes medievalistas europeos. Fue, y sigue siendo, un motor de la importante reforma en la historia producida desde los sesenta. En sus primeros trabajos se acusa la recepción de las ideas económicas y geográficas de la mejor historiografía. Su riguroso estudio sobre la base material de la Edad Media, le permitirá luego irrumpir en la historia de las mentalidades, analizando, como decía Febvre, el utillaje mental (vocabulario, sintaxis, lugares comunes, cuadros lógicos, etc. del Medioevo. Así, el ejemplo de Mauss y LéviStrauss le anima a trabajar sobre el matrimonio, la sexualidad y ciertos sistemas del pensamiento: el primero, con su defensa de los hechos sociales totales, y el segundo, que buscaba las dimensiones simbólicas de lo social, le impulsan a trabajar sobre la ideología entendida no como mero reflejo de la situación material sino como «proyecto de acción sobre lo vivido». A su obra individual, atenta a los impulsos culturales más vivos, se suma su empuje decisivo en la realización de proyectos colectivos como la Historia de la vida privada o la Historia de las mujeres. Prácticamente, han sido traducidos todos sus libros al castellano, y han podido verse en España asimismo varios de sus programas televisivos (ha sido presidente de la SEPT, cadena de televisión cultural fundada en 1985. La amplitud de sus intereses intelectuales, transmitidos en su obra con un lenguaje a la vez muy claro y bellamente elaborado, se hace palpable también en este diálogo.

  10. Aids e controle de infecção cruzada na prática odontológica: percepção e atitudes dos pacientes

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    DISCACCIATI José Augusto César

    1999-01-01

    Full Text Available Com o objetivo de avaliar a percepção dos pacientes quanto ao risco de se contrair o vírus da imunodeficiência humana (HIV durante o atendimento odontológico e suas atitudes em relação a continuar ou não o seu tratamento caso venham a saber que seu cirurgião-dentista (CD atende pacientes com AIDS ou que seja HIV soropositivo, foram realizadas 518 entrevistas entre militares da Polícia Militar de Minas Gerais e seus dependentes, que haviam terminado tratamento com 233 CD diferentes. Observou-se que a grande maioria dos participantes (88,4% acredita que o HIV possa ser transmitido durante o tratamento odontológico e que muitos entrevistados não continuariam o tratamento se seu CD atendesse pacientes com AIDS (42,9% ou fosse HIV soropositivo (45%. Aqueles entrevistados que haviam sido atendidos por profissionais que utilizavam os artigos do equipamento para proteção individual (EPI de forma adequada se mostraram mais dispostos a continuar o tratamento caso viessem a saber que seu CD atende pacientes com AIDS. No entanto, o uso adequado do EPI não é um fator encorajador para que os pacientes continuem tratando-se com seu CD caso venham a saber que ele é HIV soropositivo. Estes achados sugerem que maiores informações devem ser repassadas para o público leigo, informando-o sobre os verdadeiros riscos de se infectar pelo HIV e suas formas de prevenção na Odontologia.

  11. El papel de la mujer en las ilustraciones de los libros de texto de Educación Primaria

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    María Teresa Terrón Caro

    2008-12-01

    Full Text Available El presente artículo es resultado de una investigación realizada en el marco del Grupo de Investigación de Educación Comparada de Sevilla, con el objetivo de estudiar el papel otorgado a la mujer a través de las ilustraciones, en los textos esco- lares correspondientes a las áreas de conocimiento del medio natural, social y cultural; lengua castellana y literatura, así como matemáticas del ciclo de educación primaria. Con el análisis del rol de la mujer y el hombre en los manuales, podemos cono- cer no sólo las ideas, los valores o las actitudes latentes en una sociedad, sino además la forma en la que los conocimientos son transmitidos en las aulas tanto a niños como a niñas. Los textos escolares se muestran así como mecanismos invisibles a través de los cuales aparecen y se refuerzan desigualdades. Conscientes de esta realidad, en estos momentos las ilustraciones recogidas en los textos escolares deben ser lo suficiente- mente representativas de los cambios sociales que se producen en los distintos con- textos y capaces de plasmar modelos reales y no estereotipados. Esta reflexión debe ser un reflejo en la práctica educativa de cada uno de los niveles educativos y de cada uno de los cursos; y así lo hemos querido comprobar analizando algunas de las herramien- tas educativas utilizadas en el nivel de primaria. Palabras clave: Educación, Género, Escuela, Desigualdad, Libros de texto. 

  12. Los determinantes de la rentabilidad primaria de los bonos de titulización hipotecaria: la influencia de las estructuras multitramo

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    Miguel Á. Peña-Cerezo

    2013-11-01

    Full Text Available Este artículo analiza los determinantes de la rentabilidad primaria de los bonos de titulización hipotecaria (conocidos en la literatura como mortgage backed securities, o MBS emitidos en España durante el periodo 1993-2011, periodo en el que el mercado español llegó a convertirse en el más importante de Europa continental. Los resultados obtenidos sobre el análisis de la población completa de MBS emitidos (262 tramos configurados sobre 94 fondos de titulización indican que la estructuración multitramo de los MBS ha ayudado a reducir el riesgo percibido global de las emisiones de MBS, mediante la generación de mercados más completos y la reducción de los problemas derivados de la existencia de asimetrías informativas implícitas en el proceso de selección de los activos transmitidos por parte de la entidad cedente. Esta reducción del riesgo percibido ha tenido un efecto directo sobre la rentabilidad ofrecida por los bonos de titulización emitidos. Además, no se encuentran evidencias de que la emisión de MBS persiga la transmisión efectiva de riesgos, más bien al contrario. Las entidades de crédito, por lo general, han retenido los tramos de primeras pérdidas, lo que ha contribuido a mantener en niveles muy bajos (por debajo de la rentabilidad de la deuda soberana la rentabilidad ofrecida por los MBS. Precisamente, el escaso diferencial ofrecido por los bonos de titulización se debe a que los tramos retenidos no han ofrecido primas de rentabilidad ajustadas al riesgo inherente.

  13. Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species Seleção de espécies de Passiflora inoculadas com o vírus do mosaico do caupi revela a imunidade de uma espécie selvagem

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    Scheila da Conceição Maciel

    2009-06-01

    Full Text Available Cowpea aphid-borne mosaic virus (CABMV is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.O vírus do mosaico do caupi (Cowpea aphid-borne mosaic virus - CABMV é um potyvirus que causa uma das mais importantes doenças do maracujazeiro no Brasil. O vírus é transmitido por diversas espécies de afídeos de maneira não persistente, não circulativa. A reação de 16 espécies de Passiflora à infecção com quatro isolados brasileiros do CABMV, por meio de inoculação mecânica foi avaliada em condições de casa-de-vegetação. Somente a espécie selvagem P. suberosa foi resistente à infecção com todos os isolados do CABMV, em dois ensaios independentes. Plantas de P. suberosa enxertadas em plantas de P. edulis f. flavicarpa infectadas com o CABMV também não desenvolveram sintomas da doença. O vírus também não foi detectado por RT-PCR nas folhas superiores das plantas, sugerindo que essa espécie é imune ao CABMV.

  14. Olive oils from Algeria: Phenolic compounds, antioxidant and antibacterial activities

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    Laincer, F.

    2014-03-01

    Full Text Available The phenolic compositions, antioxidant and antimicrobial activities against six bacteria of phenolic extracts of olive oil varieties from eleven Algerian varieties were investigated. The antioxidant activity was assessed by determining the scavenging effect on the DPPH and ABTS.+ radicals. The antimicrobial activity was measured as a zone of inhibition and minimum inhibitory concentration (MIC on human harmful and foodborne pathogens. The results show that total phenols was significantly (p .+ radicals (r = 0.76. Among the bacteria tested, S. aureus and to a lesser extent B. subtilis showed the highest sensitivity; the MIC varied from 0.6 to 1.6 mg·mL-1 and 1.2 to 1.8 mg·mL-1, respectively. The results reveal that Algerian olive oils may constitute a good source of antioxidant and antimicrobial agents.Se ha estudiado la composición fenólica y las actividades antioxidante y antimicrobiana, contra seis bacterias, de extractos de aceites de oliva de once variedades argelinas. La actividad antioxidante se evaluó mediante la determinación del efecto captador de radicales de DPPH y ABTS.+. La actividad antimicrobiana se midió como zona de inhibición y como concentración inhibitoria mínima (MIC sobre bacterias perjudiciales humanas y agentes patógenos transmitidos por los alimentos. Los resultados mostraron que los fenoles totales está significativamente (p .+ (r= 0,76. Entre las bacterias ensayadas, S. aureus y, en menor grado B. subtilis mostraron la mayor sensibilidad; el MIC varió de 0,6 a 1,6 mg·mL-1 y 1,2 a 1,8 mg·mL-1 respectivamente. Los resultados muestran que los aceites de oliva argelinos pueden constituir una buena fuente de antioxidantes y agentes antimicrobianos.

  15. VIH: Transmisión Vertical y Síndrome Neurológico en Infantes

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    Jean Paul Astrulla Santa Cruz

    2009-12-01

    Full Text Available La epidemia del VIH/SIDA dejó de ser una enfermedad exclusivamente de homosexuales, para convertirse enuna enfermedad que ataca al ser humano sin distinción de raza, sexo o edad. El sexo femenino está cada vez másexpuesto a la infección y es capaz de transmitir el VIH a su hijo si no hay una intervención terapéutica adecuadaen el período de gestación. Se han descrito tres tiempos en los cuales la transmisión vertical puede ocurrir: transmisiónintraútero, transmisión intraparto, transmisión post parto vía lactancia materna. Dentro las complicacionesque presenta la transmisión madre–niño, del VIH, se presentan alteraciones neurológicas, estas pueden ser muyacentuadas y la primera manifestación es una franca detención en la evolución del crecimiento, a ello se asociaun notable retraso en el desarrollo psicomotor y pronto se establecen los síndromes de las grandes vías, que seexpresan por: rigidez extra piramidal, ataxia, síndrome piramidal. Presentamos el caso clínico, de un infante de 4meses de edad VIH positivo, transmitido de forma vertical, con neumonía, asociada a una protuberancia a nivelcefálico en la región frontal. El mismo paciente fue tratado con medidas de sostén y recuperación.

  16. Distribución de Lutzomyia longipalpis en el Chaco Argentino, 2010

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    Oscar D. Salomón

    2011-06-01

    Full Text Available El riesgo de leishmaniasis visceral (LV urbana epidémica se registró por primera vez en la Argentina en el año 2004, por presencia del vector Lutzomyia longipalpis en la provincia de Formosa, la provincia de Misiones registra casos de LV humana, LV canina y vector en el año 2006, y la provincia de Corrientes en el verano 2008-2009. En la provincia de Santiago del Estero los casos de LV humana y LV canina en el año 2008 estuvieron asociados posiblemente a vectores secundarios. Por ello, para conocer la distribución del riesgo en la región del Chaco, entre enero y abril del 2010 se realizó la búsqueda sistemática del vector de LV en 30 localidades de las provincias de Formosa, Chaco y en la ciudad de Reconquista, Santa Fe (224 trampas/noche. Se comprobó la presencia de Lu. longipalpis, por primera vez, en las localidades de Resistencia y Puerto Antequera (Chaco. En Clorinda y Puerto Pilcomayo (Formosa se obtuvieron las trampas con más ejemplares, 158 y 241 Lu. longipalpis trampa/sitio/noche respectivamente. Los resultados muestran que el vector de la LV urbana epidémica continúa dispersándose en el territorio argentino, habiendo ingresado a la provincia de Chaco. La notificación de casos esporádicos en la región chaqueña, transmitidos por vectores secundarios, como Lu. migonei, podría aumentar también debido a la vigilancia intensificada, y a la dispersión del parásito asociada al tránsito de perros infectados, sintomáticos o asintomáticos.

  17. En búsqueda de un cuidado universal y cultural Finding a Universal and cultural care

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    Cecilia Rohrbach

    2007-03-01

    Full Text Available La responsabilidad analítica que cada uno de nosotros tiene, se hace presente cada vez que se elabora una teoría relativa al cuidado, ya que construir y confirmar, o invalidar postulados, contribuye al avance de la ciencia. La crítica, en su sentido original de analizar, es también parte de la ciencia, al confirmarla enriqueciendo legítimamente su conocimiento y aplicación. El cuidado transcultural, creado y transmitido principalmente por enfermeras americanas, se origina en la antropología americana. Conviene reflexionar sobre esta perspectiva para evitar privilegiar el estudio de grupos culturales específicos, empobreciendo la investigación de grupos de cultura occidental. Esta situación motiva a analizar algunos aspectos de la antropología americana desde una perspectiva europea, para unir ambas posiciones y proponer un cuidado universal y cultural.Every one hold analytical responsibility when a caring theory is shown be cause building and confirming or invalidating postulates contributes to the progress of science Criticism, in its original analysis sense, it is part of science as well as confirming and enriching legitimately its khowledge and application. Transcultural care formed and mainly transmitted by American nurses, it is originated within American anthropology. It is convenient to reflect, in order to elude privileging specific cultural group’s studies, impoverishing those groups of a western culture. This specific situation encourages analyzing some aspects of the American anthropology with a European perspective attaching those positions and proposing a universal and cultural care.

  18. Influência familiar na anorexia nervosa: em busca das melhores evidências científicas

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    Élide Dezoti Valdanha

    2013-09-01

    Full Text Available OBJETIVO: Analisar as evidências científicas sobre a influência do grupo familiar na sintomatologia da anorexia nervosa (AN. MÉTODOS: Revisão sistemática da literatura no período de 2000 a 2012, utilizando as bases PubMed, CINAHL, PsycINFO, Lilacs e os descritores: "transtornos da alimentação", "relações familiares", "relação entre gerações" e "relação mãe-filho". RESULTADOS: Com base nos critérios de inclusão e exclusão, foram selecionadas e analisadas 20 publicações na íntegra. A maior parte dessas publicações focaliza mulheres diagnosticadas com transtornos alimentares (TA ou com comportamento alimentar de risco, tem delineamento quantitativo e utiliza escalas padronizadas para coleta de dados. Predominou o Nível de Evidência 4, que corresponde a estudos de desenho não experimental, como pesquisa descritiva correlacional e qualitativa, ou estudos de caso. As evidências encontradas indicam que os relacionamentos familiares exercem impacto significativo tanto no desenvolvimento como na manutenção de sintomas de TA. Padrões relacionados à alimentação e ao alimento são transmitidos entre as gerações, bem como aspectos não elaborados do funcionamento psíquico materno. CONCLUSÕES: Os estudos sugerem a necessidade de ampliar o foco da atenção para incluir os familiares no tratamento, bem como incorporar os achados de transmissão psíquica intergeracional como subsídios norteadores do planejamento e qualificação do cuidado oferecido nos TA.

  19. Las traducciones en la antigüedad

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    Fernández Marcos, Natalio

    2007-12-01

    Full Text Available A large part of the Western cultural heritage has been transmitted through translation. The author analyses the translation phenomenon in Antiquity, emphasizing the significance of the first translation of the Hebrew Bible into Greek in Ptolemaic Alexandria. This translation is the major literary corpus translated into Greek in Antiquity and, probably, the most important as well, due to the impact it had on the West once Christianity adopted the Greek version as the official Bible. He also describes the context in which the translation was carried out, the different models utilized for the Pentateuch, its reception and influence on other translations. Finally, he points out other processes of cultural transmission through translation in late Antiquity.

    Gran parte del legado cultural de Occidente se nos ha transmitido en traducciones. El autor analiza el fenómeno de la traducción en la Antigüedad, deteniéndose en la primera traducción de la Biblia hebrea al griego en la Alejandría ptolemaica. Esta traducción es el mayor corpus de litert ratura traducido al griego en la Antigüedad y, tal vez, el más importante por el impacto que tuvo en Occidente al ser adoptada la Biblia griega como Biblia oficial del cristianismo. Describe tambt bién el contexto en el que se llevó a cabo la traducción, los modelos utilizados en el Pentateuco, la recepción y los efectos de la misma en otras traducciones. Señala por fin otros procesos de trasvase cultural mediante la traducción en la Antigüedad tardía.

  20. Estado educador: uma nova pedagogia da hegemonia nas reservas extrativistas Educator state: a new pedagogy of hegemony in extractive reserves

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    Cláudia Conceição Cunha

    2012-06-01

    Full Text Available Este artigo analisa o papel desempenhado por um projeto de cooperação internacional (Projeto Resex na implementação das Reservas Extrativistas na década de 1990. Através da análise dos documentos e relatórios referentes à negociação e execução da primeira fase do Projeto, no período de 1995-1999, demonstra-se a contraposição dos princípios contidos nos sujeitos coletivos que originaram as Reservas Extrativistas e aqueles produzidos, transmitidos e difundidos através do Projeto Resex. Discute-se a presença do Estado, em uma pedagogia da hegemonia, operando na desconstrução das conquistas objetivadas pelos seringueiros nas décadas de 1970-1980, e utilizando, para isso, o Projeto Resex como um espaço pedagógico privilegiado.This article analyzes the role performed by an international cooperation project (Projeto Resex in the implementation of Extractive Reserves in the 1990s. Through the analysis of documents and reports referring to the negotiation and execution of the first phase of the Project in 1995-1999, the paper demonstrates the conflicting principles held by the collective subjects that participated in the formation of the Extractive Reserves and those produced, transmitted and promoted through the Projeto Resex. The presence of the state is discussed, found in a pedagogy of hegemony, operating in the deconstruction of the conquests sought by the rubber tappers in the 1970s and 1980s, and using Project Resex as a special pedagogic space.

  1. Conflicto colonial andino y mediación teológica en la crónica de Guamán Poma

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    Fernando Amaya Farías

    2012-06-01

    Full Text Available La Nueva corónica y buen gobierno de Felipe Guamán Poma de Ayala representa un documento de protesta muy singular en palabras e imágenes, como defensa de los indígenas maltratados y explotados por el sistema colonial. El cronista como indígena cristiano critica a los funcionarios políticos y religiosos de la colonia, posición que no implica necesariamente la ruptura con el sistema colonial. Para Guamán Poma, lo que amenaza la vida de las culturas indígenas no es la predicación de la salvación ni la forma ideal de vida cristiana, sino las concreciones históricas con las que el cristianismo es transmitido en la Colonia. La mediación teológica que plantea el cronista la formula desde la pregunta por la ruptura y continuidad, por la compatibilidad e incompatibilidad que se presenta entre las religiones andinas y el cristianismo.La nueva corónica y buen gobierno by Felipe Guamán Poma de Ayala represents a document of protest outstanding in words and images for the defense of the oppressed natives. The writer’s own experience as native and Christian is the startingpoint for his citizism of the colonial system. In his opinion the negative influence on the native culture wasn’t caused by the Christian promise of salvation nor by the proclaimed ideal way of Christian living, but by the way Christianity was transformed through the colonial context. The author´s main interest is to raise the question of the interruption and continuance and the compatibility and incompatibility between the native religions and Christianity.

  2. Estructura de la margen norte del Río Grande, Bardas Blancas, provincia de Mendoza

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    D.J. Dicarlo

    2007-06-01

    Full Text Available La estructura de Bardas Blancas comprende una región sobreelevada tanto topográfica como estructuralmente, conformada principalmente por estructuras braquianticlinales. Afloran en su núcleo rocas del Grupo Choiyoi y hacia los flancos depósitos sedimentarios jurásicos. El área se encuentra en un frente orogénico no emergente caracterizado por el dominio de zonas de plegamiento, fallas ciegas y el desarrollo de una zona triangular. El estudio de estas estructuras con información de subsuelo y superficie permitió el desarrollo de una sección estructural balanceada basada en el modelo de trishear. La sección se basa en el levantamiento de la estructura a partir del desarrollo de fallas de corrimiento de bajo ángulo con vergencia al este despegadas dentro del basamento. El avance de los bloques de basamento habría transmitido parte del acortamiento a la cubierta sedimentaria formándose la zona triangular de Cerro Doña Juana hacia el este. Dentro de la zona triangular se desarrolla un sistema de duplex de techo pasivo generado a partir de un nivel de despegue inferior y otro superior o retrocorrimiento. El retrocorrimiento y los Grupos Rayoso y Neuquén se encuentran volcados, producto del arrastre originado por un corrimiento de vergencia oriental. Hacia el este del retrocorrimiento las secuencias cretácicas superior y terciarias se encuentran desacopladas estructuralmente del Grupo Mendoza.

  3. ‘Everybody knows’, but the rest of the world: the case of a caterpillar-borne reproductive loss syndrome in dromedary camels observed by Sahrawi pastoralists of Western Sahara

    Science.gov (United States)

    2013-01-01

    índrome de pérdida reproductiva en dromedarios transmitido por orugas y observado por pastores nómadas saharauis del Sáhara Occidental. Antecedentes Los conocimientos tradicionales de las comunidades locales de todo el mundo son una valiosa fuente de nuevas ideas e información para la ciencia. En este estudio, se utilizaron los conocimientos de etnoveterinaria de pastores saharauis del Sáhara Occidental con el fin de proponer una hipótesis científica sobre las interacciones competitivas entre los camellos y las orugas en el ecosistema del Sáhara. Métodos Entre los años 2005 y 2009, se realizaron 44 entrevistas semi-estructuradas a los pastores saharauis en los territorios administrados por la República Árabe Saharaui Democrática, Sáhara Occidental, mediante un diseño de muestreo por bola de nieve. Resultados Los pastores nómadas saharauis describieron un síndrome reproductivo transmitido por orugas, llamado duda, entre sus camellas. Sobre la base de los conocimientos saharauis sobre el duda y una revisión literaria exhaustiva, se propuso la siguiente hipótesis: 1) brotes esporádico de orugas de la familia Lasiocampidae (géneros Lasiocampa, Psilogaster o Streblote) en árboles de Acacia se pueden presentar después de fuertes lluvias en el ecosistema del Sáhara Occidental; 2) durante estos brotes, los camellos ingieren las orugas durante el pastoreo; 3) como consecuencia de esta ingestión, se producen abortos repentinos o partos de crías debilitadas. Apoyamos esta hipótesis mediante razonamiento inductivo basado en evidencia circunstancial y razonamiento analógico con síndromes similares en yeguas de los Estados Unidos y Australia. Conclusiones Este es el primer reporte de la posible existencia de un síndrome de pérdida reproductiva en camellos, transmitido por orugas. Se insinúa que estos síndromes son más comunes que lo que actualmente se conoce. Se sugieren investigaciones adicionales para poner a prueba nuestra hipótesis. Finalmente, se

  4. ‘Everybody knows’, but the rest of the world: the case of a caterpillar-borne reproductive loss syndrome in dromedary camels observed by Sahrawi pastoralists of Western Sahara

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    Volpato Gabriele

    2013-01-01

    mundo: el caso de un síndrome de pérdida reproductiva en dromedarios transmitido por orugas y observado por pastores nómadas saharauis del Sáhara Occidental. Antecedentes Los conocimientos tradicionales de las comunidades locales de todo el mundo son una valiosa fuente de nuevas ideas e información para la ciencia. En este estudio, se utilizaron los conocimientos de etnoveterinaria de pastores saharauis del Sáhara Occidental con el fin de proponer una hipótesis científica sobre las interacciones competitivas entre los camellos y las orugas en el ecosistema del Sáhara. Métodos Entre los años 2005 y 2009, se realizaron 44 entrevistas semi-estructuradas a los pastores saharauis en los territorios administrados por la República Árabe Saharaui Democrática, Sáhara Occidental, mediante un diseño de muestreo por bola de nieve. Resultados Los pastores nómadas saharauis describieron un síndrome reproductivo transmitido por orugas, llamado duda, entre sus camellas. Sobre la base de los conocimientos saharauis sobre el duda y una revisión literaria exhaustiva, se propuso la siguiente hipótesis: 1 brotes esporádico de orugas de la familia Lasiocampidae (géneros Lasiocampa, Psilogaster o Streblote en árboles de Acacia se pueden presentar después de fuertes lluvias en el ecosistema del Sáhara Occidental; 2 durante estos brotes, los camellos ingieren las orugas durante el pastoreo; 3 como consecuencia de esta ingestión, se producen abortos repentinos o partos de crías debilitadas. Apoyamos esta hipótesis mediante razonamiento inductivo basado en evidencia circunstancial y razonamiento analógico con síndromes similares en yeguas de los Estados Unidos y Australia. Conclusiones Este es el primer reporte de la posible existencia de un síndrome de pérdida reproductiva en camellos, transmitido por orugas. Se insinúa que estos síndromes son más comunes que lo que actualmente se conoce. Se sugieren investigaciones adicionales para poner a prueba nuestra hip

  5. Gene S characterization of Hantavirus species Seoul virus isolated from Rattus norvegicuson an Indonesian island

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    Dian Perwitasari

    2014-08-01

    Full Text Available AbstrakLatar belakang: Hantavirus hidup dan berkembang biak di tubuh hewan pengerat, salah satunya Rattus norvegicus yang banyak ditemukan di daerah kepulauan di Indonesia. Hantavirus spesies Seoul virus (SEOV adalah virus RNA negatif rantai tunggal yang termasuk dalam keluarga Bunyaviridae, mempunyai beberapa gen spesifik terutama gen S yang dapat dikembangkan untuk uji diagnostik. Tujuan penelitian ini ialah untuk mengetahui karakter dari gen S dari Hantavirus spesies Seoulvirus.Metode:Pada penelitian ini dilakukan sekuensing gen S yang berasal dari jaringan paru-paru rodensia.  Fragmen DNA yang disekuensing menggunakan primer DNA SEOS-28F danSEOS -360R,VNS-1501F dan VNS-CSR. Hasil sekuensing dianalisis menggunakan program seqscapedan dianalisis menggunakan program Bioedit dan Mega5. Analisis filogenetik untuk homologi nukleotida dan asam amino dari ketiga strain Kepulauan Seribu tersebut dibandingkan dengan spesies hantavirus lainnya yang diambil dari genebank. Hasil:Analisis Homologi nukleotida dan asam amino antara strain Kepulauan Seribu dengan SEOV menunjukkan homologi nukleotida tertinggi pada strain KS74 (88,4% dan terendah pada KS90 (87,2%, sedangkan homologi asam amino tertinggi adalah strain KS74 (91.3% dan terendah pada strain KS90 (89,5%. Kesimpulan:Karakter gen S virus yang ditemukan di Kepulauan Seribu sebanding dengan virus SEOV yang ditemukan di Singapura dan Korea.  (Health Science Indones 2014;1:1-6Kata kunci:Seoul virus, gen S, Kepulauan Seribu, IndonesiaAbstractBackground: Hantavirus lives and reproduces in the body of rodents. Rattus norvegicuswas one found in the Kepulauan Seribu islands of Indonesia. Hantavirus species Seoul virus (SEOV is a negative single chain RNA viruses included in the family Bunyaviridae. It has a few specific genes, especially genes S that can be developed for a diagnostic test. The aim of this study was to ascertain the character of gene S of hantavirus species Seoul virus. Methods: Gene

  6. Epidemia de febre do Oropouche em Serra Pelada, município de Curionópolis, Pará, 1994

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    Amélia P.A.T. Rosa

    1996-12-01

    Full Text Available No final de novembro de 1994, o Instituto Evandro Chagas (IEC, Belém, Pará, foi notificado de um surto de doença febril na população do garimpo de Serra Pelada, município de Curionôpolis (5°35'S; 49°30'W, no Estado do Pará. Vinte amostras de soro de pessoas, com hemoscopia negativa para tnalária, foram recebidas para esclarecimento diagnóstico. Estudos laboratoriais comprovaram que os casos eram devido ao vírus Oropouche (grupo Simbu. gênero Bunyavirus, família Bunyaviridae. Esses achados, induziram d ida de um grupo de técnicos para realização de investigações ecoepidemíológicas entre 8 e 22 de dezembro. Foram coletadas 296 amostras de sangue, de 73 grupos familiares, sendo 54 para pequisa de vírus (casos febris e 242para sorologia, bem como, procedeu-se a coleta de artrópodes hematófagos. As amostras para pesquisa de vírus foram inoculadas em camundongos recém-nascidos e os soros testados por inibição da hemaglutinação (1H e MAC ELISA. Foram isoladas dez amostras do vírus Oropouche e obtidas seis soroconversões. Ademais, 245 (82,8% amostras foram positivas por sorologia e 71 (97,3% grupos familiares apresentaram pelo menos um membro positivo. Considerando a elevada positividade de anticoipos IH e IgM específica para Oropouche na população de Serra Pelada, concluímos que a epidemia foi extensa e apresentou taxa de ataque em torno de 83%, que correspondeu a infecção de cerca de 5.000 pessoas.In the final of November 1994, an outbreak of a febrile disease was observed in the Serra Pelada gold mine (5°35'S; 49°30'W in the Southeast region of Parã State. Twenty samples were collected and sent to the laboratory of Arbovirus of Instituto Evandro Chagas. The tests showed that the disease was caused by Oropouche virus (Bunyaviridae, Bunyavirus, Simbu serological group. Between 8-22 December 296 serum samples mere taken (54 from febrile patients, 16 paired samples and 242 from contacts and convalescent patients

  7. Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana

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    Calisher Charles H

    2009-07-01

    Full Text Available Abstract Background All viruses in the family Bunyaviridae possess a tripartite genome, consisting of a small, a medium, and a large RNA segment. Bunyaviruses therefore possess considerable evolutionary potential, attributable to both intramolecular changes and to genome segment reassortment. Hantaviruses (family Bunyaviridae, genus Hantavirus are known to cause human hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. The primary reservoir host of Sin Nombre virus is the deer mouse (Peromyscus maniculatus, which is widely distributed in North America. We investigated the prevalence of intramolecular changes and of genomic reassortment among Sin Nombre viruses detected in deer mice in three western states. Methods Portions of the Sin Nombre virus small (S and medium (M RNA segments were amplified by RT-PCR from kidney, lung, liver and spleen of seropositive peromyscine rodents, principally deer mice, collected in Colorado, New Mexico and Montana from 1995 to 2007. Both a 142 nucleotide (nt amplicon of the M segment, encoding a portion of the G2 transmembrane glycoprotein, and a 751 nt amplicon of the S segment, encoding part of the nucleocapsid protein, were cloned and sequenced from 19 deer mice and from one brush mouse (P. boylii, S RNA but not M RNA from one deer mouse, and M RNA but not S RNA from another deer mouse. Results Two of 20 viruses were found to be reassortants. Within virus sequences from different rodents, the average rate of synonymous substitutions among all pair-wise comparisons (πs was 0.378 in the M segment and 0.312 in the S segment sequences. The replacement substitution rate (πa was 7.0 × 10-4 in the M segment and 17.3 × 10-4 in the S segment sequences. The low πa relative to πs suggests strong purifying selection and this was confirmed by a Fu and Li analysis. The absolute rate of molecular evolution of the M segment was 6.76 × 10-3 substitutions/site/year. The absolute age of the M segment

  8. Serologic survey of hantavirus in a rural population from the northern State of Mato Grosso, Brazil Pesquisa sorológica para hantavírus em uma população rural do norte do Estado do Mato Grosso, Brasil

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    Ioni Oliveira Santos

    2012-01-01

    Full Text Available INTRODUCTION: Hantavirus is a genus of ribonucleic acid (RNA viruses included in the family Bunyaviridae. Hantaviruses are rodent-borne zoonoses that, in the last 18 years, became an emergent public health problem in the Americas, causing a severe cardiopulmonary syndrome. This disease has no specific treatment and has a high case fatality. The transmission of hantavirus to man occurs by inhaling aerosols of rodent excreta. The aim of this study was to determine the prevalence of antibodies to hantavirus in the population of the rural settlement of Tupã in the county of Marcelândia, State of Mato Grosso, Brazil. METHODS: The participants of the serologic survey were visited at their homes and selected randomly among the settlement population. Blood samples of the participants were collected by venopuncture. The serum samples were tested by an IgG-ELISA using an N recombinant protein of Araraquara hantavirus as antigen, using the protocol previously established by Figueiredo et al. RESULTS: IgG antibodies to hantavirus were detected in 7 (13% of the 54 participants. The positivity was higher among men. It was observed that there was an association of seropositivity to hantavirus within the participants born in the south of Brazil. CONCLUSIONS: The results suggest that, in this rural area, everyone is exposed to the same risk of becoming infected with hantavirus, and, therefore, there is a need to intensify surveillance activities and education of the local people to prevent this viral infection.INTRODUÇÃO: Hantavirus é um gênero de vírus RNA incluído na família Bunyaviridae. Hantaviroses são zoonoses transmitidas por roedores que nos últimos 18 anos tornou-se um problema emergente da saúde pública nas Américas causando uma síndrome cardiopulmonar. Esta doença não tem nenhum tratamento específico e apresenta alta letalidade. A transmissão do hantavirus ao homem ocorre pela inalação de aerossóis dos excrementos de roedores. O

  9. Genetic Diversity of Crimean Congo Hemorrhagic Fever Virus Strains from Iran

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    Sadegh Chinikar

    2016-01-01

    Full Text Available Background: Crimean Congo hemorrhagic fever virus (CCHFV is a member of the Bunyaviridae family and Nairovirus genus. It has a negative-sense, single stranded RNA genome approximately 19.2 kb, containing the Small, Medium, and Large segments. CCHFVs are relatively divergent in their genome sequence and grouped in seven distinct clades based on S-segment sequence analysis and six clades based on M-segment sequences. Our aim was to obtain new insights into the molecular epidemiology of CCHFV in Iran.Methods: We analyzed partial and complete nucleotide sequences of the S and M segments derived from 50 Iranian patients. The extracted RNA was amplified using one-step RT-PCR and then sequenced. The sequences were ana­lyzed using Mega5 software.Results: Phylogenetic analysis of partial S segment sequences demonstrated that clade IV-(Asia 1, clade IV-(Asia 2 and clade V-(Europe accounted for 80 %, 4 % and 14 % of the circulating genomic variants of CCHFV in Iran respectively. However, one of the Iranian strains (Iran-Kerman/22 was associated with none of other sequences and formed a new clade (VII. The phylogenetic analysis of complete S-segment nucleotide sequences from selected Ira­nian CCHFV strains complemented with representative strains from GenBank revealed similar topology as partial sequences with eight major clusters. A partial M segment phylogeny positioned the Iranian strains in either associa­tion with clade III (Asia-Africa or clade V (Europe.Conclusion: The phylogenetic analysis revealed subtle links between distant geographic locations, which we pro­pose might originate either from international livestock trade or from long-distance carriage of CCHFV by infected ticks via bird migration.

  10. Structural Analysis of Monomeric RNA-Dependent Polymerases: Evolutionary and Therapeutic Implications.

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    Rodrigo Jácome

    Full Text Available The crystal structures of monomeric RNA-dependent RNA polymerases and reverse transcriptases of more than 20 different viruses are available in the Protein Data Bank. They all share the characteristic right-hand shape of DNA- and RNA polymerases formed by the fingers, palm and thumb subdomains, and, in many cases, "fingertips" that extend from the fingers towards the thumb subdomain, giving the viral enzyme a closed right-hand appearance. Six conserved structural motifs that contain key residues for the proper functioning of the enzyme have been identified in all these RNA-dependent polymerases. These enzymes share a two divalent metal-ion mechanism of polymerization in which two conserved aspartate residues coordinate the interactions with the metal ions to catalyze the nucleotidyl transfer reaction. The recent availability of crystal structures of polymerases of the Orthomyxoviridae and Bunyaviridae families allowed us to make pairwise comparisons of the tertiary structures of polymerases belonging to the four main RNA viral groups, which has led to a phylogenetic tree in which single-stranded negative RNA viral polymerases have been included for the first time. This has also allowed us to use a homology-based structural prediction approach to develop a general three-dimensional model of the Ebola virus RNA-dependent RNA polymerase. Our model includes several of the conserved structural motifs and residues described in other viral RNA-dependent RNA polymerases that define the catalytic and highly conserved palm subdomain, as well as portions of the fingers and thumb subdomains. The results presented here help to understand the current use and apparent success of antivirals, i.e. Brincidofovir, Lamivudine and Favipiravir, originally aimed at other types of polymerases, to counteract the Ebola virus infection.

  11. Serologic evidence of Jamestown Canyon and Keystone virus infection in vertebrates of the DelMarVa Peninsula.

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    Watts, D M; LeDuc, J W; Bailey, C L; Dalrymple, J M; Gargan, T P

    1982-11-01

    Serological data accumulated during the past decade indicated that a variety of feral and domestic animals of the Delaware-Maryland-Virginia (DelMarVa) Peninsula were infected with Jamestown Canyon (JC) and/or Keystone (KEY) viruses (Bunyaviridae, California serogroup). Neutralizing (N) antibody to JC virus was most prevalent in white-tailed deer, sika deer, cottontail rabbits and horses. KEY virus N antibody was detected most frequently in gray squirrels and domestic goats. N antibody indicative of past infection by one or both viruses also was found in raccoons, horses and humans. JC and/or KEY virus N antibodies were not demonstrable in sera of several other species of small mammals and reptiles. Investigations were extended to evaluate the role of domestic goats as an amplifying host of JC and KEY viruses and to assess their potential as sentinels of virus transmission. Goats maintained in the Pocomoke Cypress Swamp during the summer season of 1978, acquired N antibodies to JC and KEY viruses. Following experimental inoculation with either JC or KEY virus, all goats developed N antibody despite the absence of a demonstrable viremia in most animals. Goats proved to be effective as sentinels for monitoring the transmission of JC and KEY viruses; however, the exceptionally low titers or absence of viremia following inoculation with these viruses would seem to preclude a potential virus-amplifying role for this species. Although findings implicated primarily gray squirrels and white-tailed deer as possible amplifying hosts of KEY and JC virus, respectively, further investigations will be required to clarify their role, particularly since both viruses may be maintained entirely by transovarial transmission. PMID:7149110

  12. Novel Camelid Antibody Fragments Targeting Recombinant Nucleoprotein of Araucaria hantavirus: A Prototype for an Early Diagnosis of Hantavirus Pulmonary Syndrome

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    Pereira, Soraya S.; Moreira-Dill, Leandro S.; Morais, Michelle S. S.; Prado, Nidiane D. R.; Barros, Marcos L.; Koishi, Andrea C.; Mazarrotto, Giovanny A. C. A.; Gonçalves, Giselle M.; Zuliani, Juliana P.; Calderon, Leonardo A.; Soares, Andreimar M.; Pereira da Silva, Luiz H.; Duarte dos Santos, Claudia N.; Fernandes, Carla F. C.; Stabeli, Rodrigo G.

    2014-01-01

    In addition to conventional antibodies, camelids produce immunoglobulins G composed exclusively of heavy chains in which the antigen binding site is formed only by single domains called VHH. Their particular characteristics make VHHs interesting tools for drug-delivery, passive immunotherapy and high-throughput diagnosis. Hantaviruses are rodent-borne viruses of the Bunyaviridae family. Two clinical forms of the infection are known. Hemorrhagic Fever with Renal Syndrome (HFRS) is present in the Old World, while Hantavirus Pulmonary Syndrome (HPS) is found on the American continent. There is no specific treatment for HPS and its diagnosis is carried out by molecular or serological techniques, using mainly monoclonal antibodies or hantavirus nucleoprotein (N) to detect IgM and IgG in patient serum. This study proposes the use of camelid VHHs to develop alternative methods for diagnosing and confirming HPS. Phage display technology was employed to obtain VHHs. After immunizing one Lama glama against the recombinant N protein (prNΔ85) of a Brazilian hantavirus strain, VHH regions were isolated to construct an immune library. VHHs were displayed fused to the M13KO7 phage coat protein III and the selection steps were performed on immobilized prNΔ85. After selection, eighty clones recognized specifically the N protein. These were sequenced, grouped based mainly on the CDRs, and five clones were analyzed by western blot (WB), surface plasmon resonance (SPR) device, and ELISA. Besides the ability to recognize prNΔ85 by WB, all selected clones showed affinity constants in the nanomolar range. Additionaly, the clone KC329705 is able to detect prNΔ85 in solution, as well as the native viral antigen. Findings support the hypothesis that selected VHHs could be a powerful tool in the development of rapid and accurate HPS diagnostic assays, which are essential to provide supportive care to patients and reduce the high mortality rate associated with hantavirus infections. PMID

  13. Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan

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    Tanaka Shogo

    2008-06-01

    Full Text Available Abstract Background Akabane virus is a member of the genus Orthobunyavirus in the family Bunyaviridae. It is transmitted by hematophagous arthropod vectors such as Culicoides biting midges and is widely distributed in temperate to tropical regions of the world. The virus is well known as a teratogenic pathogen which causes abortions, stillbirths, premature births and congenital abnormalities with arthrogryposis-hydranencephaly syndrome in cattle, sheep and goats. On the other hand, it is reported that the virus rarely induces encephalomyelitis in cattle by postnatal infection. A first large-scale epidemic of Akabane viral encephalomyelitis in cattle occurred in the southern part of Japan from summer to autumn in 2006. The aim of this study is to define the epidemiological, pathological and virological properties of the disease. Results Nonsuppurative encephalomyelitis was observed in cattle that showed neurological symptoms such as astasia, ataxia, opisthotonus and hypersensitivity in beef and dairy farms by histopathological analysis. Akabane viral antigen and genome were consistently detected from the central nervous system of these animals, and the virus was isolated not only from them but also from the blood samples of clinically healthy calves in the epidemic area. The isolates were classified into genogroup I a containing the Iriki strain, which caused encephalitis of calves almost twenty years ago in Japan. Most of the affected cattle possessed the neutralizing antibody against Akabane virus. Seroconversion of the cohabitated and sentinel cattle in the epidemic area was also confirmed during an outbreak of the disease. Conclusion The ecological and epidemiological data we have obtained so far demonstrated that the Akabane virus is not endemic in Japan. No evidence of Akabane virus circulation was observed in 2005 through nation-wide serological surveillance, suggesting that a new strain belonging to genogroup I a invaded southern Japan

  14. Inhibition of Hazara nairovirus replication by small interfering RNAs and their combination with ribavirin

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    Crance Jean-Marc

    2011-05-01

    Full Text Available Abstract Background The genus Nairovirus in the family Bunyaviridae contains 34 tick-borne viruses classified into seven serogroups. Hazara virus (HAZV belongs to the Crimean-Congo hemorrhagic fever (CCHF serogroup that also includes CCHF virus (CCHFV a major pathogen for humans. HAZV is an interesting model to study CCHFV due to a close serological and phylogenetical relationship and a classification which allows handling in a BSL2 laboratory. Nairoviruses are characterized by a tripartite negative-sense single stranded RNA genome (named L, M and S segments that encode the RNA polymerase, the Gn-Gc glycoproteins and the nucleoprotein (NP, respectively. Currently, there are neither vaccines nor effective therapies for the treatment of any bunyavirus infection in humans. In this study we report, for the first time, the use of RNA interference (RNAi as an approach to inhibit nairovirus replication. Results Chemically synthesized siRNAs were designed to target the mRNA produced by the three genomic segments. We first demonstrated that the siRNAs targeting the NP mRNA displayed a stronger antiviral effect than those complementary to the L and M transcripts in A549 cells. We further characterized the two most efficient siRNAs showing, that the induced inhibition is specific and associated with a decrease in NP synthesis during HAZV infection. Furthermore, both siRNAs depicted an antiviral activity when used before and after HAZV infection. We next showed that HAZV was sensitive to ribavirin which is also known to inhibit CCHFV. Finally, we demonstrated the additive or synergistic antiviral effect of siRNAs used in combination with ribavirin. Conclusions Our study highlights the interest of using RNAi (alone or in combination with ribavirin to treat nairovirus infection. This approach has to be considered for the development of future antiviral compounds targeting CCHFV, the most pathogenic nairovirus.

  15. Comparative analysis of the L, M, and S RNA segments of Crimean-Congo haemorrhagic fever virus isolates from southern Africa.

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    Goedhals, Dominique; Bester, Phillip A; Paweska, Janusz T; Swanepoel, Robert; Burt, Felicity J

    2015-05-01

    Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Bunyaviridae family with a tripartite, negative sense RNA genome. This study used predictive software to analyse the L (large), M (medium), and S (small) segments of 14 southern African CCHFV isolates. The OTU-like cysteine protease domain and the RdRp domain of the L segment are highly conserved among southern African CCHFV isolates. The M segment encodes the structural glycoproteins, GN and GC, and the non-structural glycoproteins which are post-translationally cleaved at highly conserved furin and subtilase SKI-1 cleavage sites. All of the sites previously identified were shown to be conserved among southern African CCHFV isolates. The heavily O-glycosylated N-terminal variable mucin-like domain of the M segment shows the highest sequence variability of the CCHFV proteins. Five transmembrane domains are predicted in the M segment polyprotein resulting in three regions internal to and three regions external to the membrane across the G(N), NS(M) and G(C) glycoproteins. The corroboration of conserved genome domains and sequence identity among geographically diverse isolates may assist in the identification of protein function and pathogenic mechanisms, as well as the identification of potential targets for antiviral therapy and vaccine design. As detailed functional studies are lacking for many of the CCHFV proteins, identification of functional domains by prediction of protein structure, and identification of amino acid level similarity to functionally characterised proteins of related viruses or viruses with similar pathogenic mechanisms are a necessary step for selection of areas for further study. PMID:25693737

  16. A Seroepidemiological Survey of Crimean Congo Hemorrhagic Fever among Goats and Sheep in Lezhe Torovica Province, Albania

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    ARTA LUGAJ

    2014-09-01

    Full Text Available Crimean-Congo hemorrhagic fever (CCHF is a zoonotic vector-born viral disease with a case fatality rate of 2-50% in human. CCHFV is classified within the Nairovirus genus in the Bunyaviridae family. The virus is endemic in over 30 countries, including Albanian. Animals can infect by virus without clinical symptoms.. The virus can be transmitted mainly through direct contact with blood or tissues from infected livestock or through bites of Hyalomma ticks. The aim of this study was to examine the distribution of CCHFV among sheep and goats in Lezha-Torovica region of Albania. This survey was carried out in 2013. In all, 50 blood samples were taken from the jugular veins of the sheep, and 50 blood samples were taken from the jugular veins of the goats. The samples were immediately taken to the laboratory and their serum extracted, separated by a centrifuge with 3500 rpm in 10 minutes. They were kept in the Faculty of Veterinary Medicine, Agricultural University of Tirana, at a temperature below 20°C until analysis and tested with immunological methods using indirect ELISA assay in Friedrich-Loeffler Institute (FLI, Greifswald Germany. Through this technique it was possible to identified IgG antibodies in infected serum samples. The prevalence of this infection in animals is obviously different, in goats is higher than in sheep, respectively 90% and 24%. It is recommended that further studies be carried out on additional livestock, high-risk groups of humans, and ticks, to determine the CCHF disease status in Lezha-Torovica.

  17. Mini-genome rescue of Crimean-Congo hemorrhagic fever virus and research into the evolutionary patterns of its untranslated regions.

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    Zhao, Jiuru; Xia, Han; Zhang, Yujiang; Yin, Shiyu; Zhang, Zhong; Tang, Shuang; Kou, Zheng; Yu, Jingfeng; Fan, Zhaojun; Li, Tianxian

    2013-10-01

    Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of genus Nairovirus, family Bunyaviridae, which are distributed widely in Africa, Europe and Asia with several genotypes. As a BSL-4 level pathogen, the requirement of high-level biosafety facilities severely constrains researches on live virus manipulation. In this study, we developed a helper-virus-independent mini-genome rescue system for the Chinese YL04057 strain. Based on the enhanced green fluorescent protein (EGFP)-derived mini-genome plasmids, this polymerase I driven system permits easy observation and quantification. Unlike previous report, gradually reduced levels of activity of the CCHFV L, M and S untranslated regions (UTRs) were observed in our system. We also demonstrated that the UTRs at both ends were indispensable for mini-genome background expression. In addition, we phylogentically analyzed all six UTRs of CCHFV and showed that L-UTRs were clustered together approximately corresponding to their original geographical continents. The UTRs of M segment showed a similar branch structure to its open reading frames (ORFs), and nearly an identical tree was generated with 5' UTRs of S segment compared with its ORFs. However, the 3' UTRs of S segment formed new divergent groups. Compatibility tests of YL04057 strain nucleocapsid protein and L protein expression plasmids with Nigerian strain IbAr10200 mini-genomes revealed lower compatibility of L-UTRs without an obvious effect on M-UTRs. Moreover, we demonstrated that the L-UTRs could tolerate certain nucleotide mutations. This system may provide a foundation for future studies of the viral replication cycle, pathogenic mechanisms and evolutionary patterns of CCHFV. PMID:23891575

  18. Epidemiological survey of Crimean Congo hemorrhagic fever virus in cattle in East Darfur State, Sudan.

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    Ibrahim, Alaa M; Adam, Ibrahim A; Osman, Badreldin T; Aradaib, Imadeldin E

    2015-06-01

    Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by CCHF virus (CCHFV) of the genus Nairovirus in the family Bunyaviridae. CCHFV causes subclinical infection in domestic livestock and an often fatal hemorrhagic illness in humans, with approximately 30% mortality rates. In the present study, a cross-sectional serosurvey was conducted in a total of 282 randomly selected cattle from five localities in East Darfur State, Sudan. The exposure status to CCHF was determined using enzyme-linked immunosorbent assay (ELISA) for detection of CCHFV-specific IgG antibodies in cattle serum samples. The CCHFV-specific IgG antibodies were detected in 54 out of 282 animals, accounting for a 19.14% prevalence rate. Older cattle (>2 years of age) were approximately five times more likely to be infected with the virus (OR=4.90, CI=1.28-18.98, p-value=0.02). Heavily tick-infested cattle (ticks all over the body) were at 11 times higher at risk compared to tick-free animals (OR=11.11, CI=2.86-43.25, p-value=0.01). Grazing system is another factor affecting CCHF, where cattle grazing on open system were 27 times more at risk compared to other grazing systems (OR=27.22, CI=7.46-99.24, p-value=0.001). There was an association between localities and CCHF cattle (OR=0.24, CI=0.07-0.83, p-value=0.02). This study confirms the exposure of cattle to CCHF in East Darfur and identifies potential risk factors associated with the disease. Further epidemiological studies and improved surveillance are urgently needed to prevent a possible outbreak of CCHF among humans in the Darfur region of Sudan. PMID:25898993

  19. Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus Reveals a Function for Non-structural Glycoproteins Cleavage by Furin.

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    Bergeron, Éric; Zivcec, Marko; Chakrabarti, Ayan K; Nichol, Stuart T; Albariño, César G; Spiropoulou, Christina F

    2015-05-01

    Crimean Congo hemorrhagic fever virus (CCHFV) is a negative-strand RNA virus of the family Bunyaviridae (genus: Nairovirus). In humans, CCHFV causes fever, hemorrhage, severe thrombocytopenia, and high fatality. A major impediment in precisely determining the basis of CCHFV's high pathogenicity has been the lack of methodology to produce recombinant CCHFV. We developed a reverse genetics system based on transfecting plasmids into BSR-T7/5 and Huh7 cells. In our system, bacteriophage T7 RNA polymerase produced complementary RNA copies of the viral S, M, and L segments that were encapsidated with the support, in trans, of CCHFV nucleoprotein and L polymerase. The system was optimized to systematically recover high yields of infectious CCHFV. Additionally, we tested the ability of the system to produce specifically designed CCHFV mutants. The M segment encodes a polyprotein that is processed by host proprotein convertases (PCs), including the site-1 protease (S1P) and furin-like PCs. S1P and furin cleavages are necessary for producing the non-structural glycoprotein GP38, while S1P cleavage yields structural Gn. We studied the role of furin cleavage by rescuing a recombinant CCHFV encoding a virus glycoprotein precursor lacking a functional furin cleavage motif (RSKR mutated to ASKA). The ASKA mutation blocked glycoprotein precursor's maturation to GP38, and Gn precursor's maturation to Gn was slightly diminished. Furin cleavage was not essential for replication, as blocking furin cleavage resulted only in transient reduction of CCHFV titers, suggesting that either GP38 and/or decreased Gn maturation accounted for the reduced virion production. Our data demonstrate that nairoviruses can be produced by reverse genetics, and the utility of our system uncovered a function for furin cleavage. This viral rescue system could be further used to study the CCHFV replication cycle and facilitate the development of efficacious vaccines to counter this biological and public

  20. La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys

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    Murphy Brian R

    2008-02-01

    Full Text Available Abstract Background La Crosse virus (LACV, family Bunyaviridae, was first identified as a human pathogen in 1960 after its isolation from a 4 year-old girl with fatal encephalitis in La Crosse, Wisconsin. LACV is a major cause of pediatric encephalitis in North America and infects up to 300,000 persons each year of which 70–130 result in severe disease of the central nervous system (CNS. As an initial step in the establishment of useful animal models to support vaccine development, we examined LACV infectivity, pathogenesis, and immunogenicity in both weanling mice and rhesus monkeys. Results Following intraperitoneal inoculation of mice, LACV replicated in various organs before reaching the CNS where it replicates to high titer causing death from neurological disease. The peripheral site where LACV replicates to highest titer is the nasal turbinates, and, presumably, LACV can enter the CNS via the olfactory neurons from nasal olfactory epithelium. The mouse infectious dose50 and lethal dose50 was similar for LACV administered either intranasally or intraperitoneally. LACV was highly infectious for rhesus monkeys and infected 100% of the animals at 10 PFU. However, the infection was asymptomatic, and the monkeys developed a strong neutralizing antibody response. Conclusion In mice, LACV likely gains access to the CNS via the blood stream or via olfactory neurons. The ability to efficiently infect mice intranasally raises the possibility that LACV might use this route to infect its natural hosts. Rhesus monkeys are susceptible to LACV infection and develop strong neutralizing antibody responses after inoculation with as little as 10 PFU. Mice and rhesus monkeys are useful animal models for LACV vaccine immunologic testing although the rhesus monkey model is not optimal.

  1. The consequences of reconfiguring the ambisense S genome segment of Rift Valley fever virus on viral replication in mammalian and mosquito cells and for genome packaging.

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    Benjamin Brennan

    2014-02-01

    Full Text Available Rift Valley fever virus (RVFV, family Bunyaviridae is a mosquito-borne pathogen of both livestock and humans, found primarily in Sub-Saharan Africa and the Arabian Peninsula. The viral genome comprises two negative-sense (L and M segments and one ambisense (S segment RNAs that encode seven proteins. The S segment encodes the nucleocapsid (N protein in the negative-sense and a nonstructural (NSs protein in the positive-sense, though NSs cannot be translated directly from the S segment but rather from a specific subgenomic mRNA. Using reverse genetics we generated a virus, designated rMP12:S-Swap, in which the N protein is expressed from the NSs locus and NSs from the N locus within the genomic S RNA. In cells infected with rMP12:S-Swap NSs is expressed at higher levels with respect to N than in cells infected with the parental rMP12 virus. Despite NSs being the main interferon antagonist and determinant of virulence, growth of rMP12:S-Swap was attenuated in mammalian cells and gave a small plaque phenotype. The increased abundance of the NSs protein did not lead to faster inhibition of host cell protein synthesis or host cell transcription in infected mammalian cells. In cultured mosquito cells, however, infection with rMP12:S-Swap resulted in cell death rather than establishment of persistence as seen with rMP12. Finally, altering the composition of the S segment led to a differential packaging ratio of genomic to antigenomic RNA into rMP12:S-Swap virions. Our results highlight the plasticity of the RVFV genome and provide a useful experimental tool to investigate further the packaging mechanism of the segmented genome.

  2. Serological survey of Crimean-Congo hemorrhagic fever virus in cattle in Berat and Kolonje, Albania

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    ARTA LUGAJ

    2014-06-01

    Full Text Available Crimean–Congo hemorrhagic fever (CCHF is a tick-borne disease caused by the arbovirus Crimean–Congo hemorrhagic fever virus(CCHFV, which is a member of the Nairovirusgenus (family Bunyaviridae. The disease now occurs sporadically throughout much of Africa, Asia, andEurope and results in an approximately 30% fatality rate. Numerous genera of ixodid ticks serve both as vector and reservoir for CCHFV; however, ticks in the genus Hyalommaare particularlyimportant to the ecology of this virus.The aim of this study was to examine the distribution of CCHFV among the cattle in Berat and Kolonje regions in Albania. The data taken in this study indicates for the presence of CCHFV Crimean-Congo hemorrhagic fever virus in these countries. The serum samples were conserved in -20°C and tested with immunological methods using indirect ELISA assay in Friedrich-Loeffler Institute (FLI, Greifswald Germany. Through this technique it was possible to identified IgG antibodies in infected serum samples. From these results in Berat-Terpanwe had an indication about the presence of IgG antibodies in 2 blood samples. 3 serum samples were equivocal and 45 serum samples were negative from the total of 50 serum samples in cattle. While in Kolonje-Erseke the results show the presence of IgG antibodies in 4 blood samples from 54 seum samples in cattle. Respectively the prevalence in these 2 countries in Albania is 4.4% and 8%. These results can clearly proved the presence of CCHFV in livestock in Albania.

  3. Life-long shedding of Puumala hantavirus in wild bank voles (Myodes glareolus).

    Science.gov (United States)

    Voutilainen, Liina; Sironen, Tarja; Tonteri, Elina; Bäck, Anne Tuiskunen; Razzauti, Maria; Karlsson, Malin; Wahlström, Maria; Niemimaa, Jukka; Henttonen, Heikki; Lundkvist, Åke

    2015-06-01

    The knowledge of viral shedding patterns and viraemia in the reservoir host species is a key factor in assessing the human risk of zoonotic viruses. The shedding of hantaviruses (family Bunyaviridae) by their host rodents has widely been studied experimentally, but rarely in natural settings. Here we present the dynamics of Puumala hantavirus (PUUV) shedding and viraemia in naturally infected wild bank voles (Myodes glareolus). In a monthly capture-mark-recapture study, we analysed 18 bank voles for the presence and relative quantity of PUUV RNA in the excreta and blood from 2 months before up to 8 months after seroconversion. The proportion of animals shedding PUUV RNA in saliva, urine and faeces peaked during the first month after seroconversion, but continued throughout the study period with only a slight decline. The quantity of shed PUUV in reverse transcription quantitative PCR (RT-qPCR) positive excreta was constant over time. In blood, PUUV RNA was present for up to 7 months but both the probability of viraemia and the virus load declined with time. Our findings contradict the current view of a decline in virus shedding after the acute phase and a short viraemic period in hantavirus infection - an assumption widely adopted in current epidemiological models. We suggest the life-long shedding as a means of hantaviruses to survive over host population bottlenecks, and to disperse in fragmented habitats where local host and/or virus populations face temporary extinctions. Our results indicate that the kinetics of pathogens in wild hosts may differ considerably from those observed in laboratory settings. PMID:25701819

  4. Crimean-Congo haemorrhagic fever: an overview

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    Virat J. Agravat

    2014-04-01

    Full Text Available Crimean-Congo Hemorrhagic Fever (CCHF is an acute, highly-contagious and life-threatening vector borne disease. The CCHF virus causes severe viral hemorrhagic fever outbreaks, with a case fatality rate of 10-40%. CCHF virus isolation and/or disease has been reported from more than 30 countries in Africa, Asia, South eastern Europe and Middle east. Jan 2011 marks first ever reports of outbreak of CCHF in India, total 5 cases were detected of CCHF from Gujarat. CCHF has recently in news again, 6 human cases and 32 animal samples test positive for CCHF from Kariyana village of Amreli district (Gujarat state July 2013. Crimean-Congo hemorrhagic fever virus (CCHFV, member of genus Nairovirus in the family Bunyaviridae. Numerous genera of ixodid ticks serve both as vector and reservoir for CCHFV. Human infections occurred through tick bites, direct contact with blood or tissue of infected livestock, or nosocomial infections. Human infections begin with nonspecific febrile symptoms, but progress to a serious hemorrhagic syndrome with a high case fatality ratio. The most definitive way of diagnosis is the demonstration of virus or viral genome in sera samples. Hospitalization in special care unit with constant effort to prevent haemorrhagic complication along with laboratory monitoring is cornerstone for treatment of CCHF. Till date there is no FDA approved drug or definitive treatment for CCHF, ribavirin is tried by many physician need to be evaluated further. Current article is an effort to update existing knowledge about CCHF by due focus on various aspects especially prevention of this zoonotic disease. Much of the real life queries about this disease are elaborated after extensive literature research. [Int J Res Med Sci 2014; 2(2.000: 392-397

  5. Bovine aortic endothelial cells are susceptible to hantavirus infection; a new aspect in hantavirus ecology

    International Nuclear Information System (INIS)

    Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can transmit the virus via feces, urine, saliva, or bites to humans. Additionally, antibodies against different hantaviruses were also found in domestic animals, For example, Danes et al. documented hantavirus-specific IgG titers in 2% of examined cattle [Ceskoslov. Epidemiol. Mikrobiol. Imunol. 41 (1992) 15]. In order to clarify the possibility of a nonrodent and nonhuman hantavirus infection, the susceptibility of bovine aortic endothelial cells (BAEC) to Hantavirus serotype Puumala infection was investigated. The hantaviral nucleocapsid protein was detected in 95% of infected BAEC at the fourth cell culture passage 12 weeks after initial infection by immunofluorescence assay (IFA). The presence of Puumala virus (PUU) nucleocapsid protein and the viral glycoproteins G1 and G2 in infected cells were additionally confirmed by Western blot analysis. The viral RNA genome was identified in infected BAEC cultures and in cell-free culture medium at the fourth passage by reverse transcription polymerase chain reaction (RT-PCR), verified by cDNA nucleotide sequence analysis, showing a 98-100% homology to the input virus. The infected BAEC cultures were shown to express αVβ3-integrin surface receptors that are known to mediate virus entry in human cells and revealed no major cytopathic effects (CPEs) as assayed by immunofluorescence staining of the cytoskeletal components actin and microtubules. In the present study, we documented for the first time that a nonrodent and nonhuman aortic endothelial cell culture of bovine origin (BAEC) can be efficiently infected with a hantavirus. This finding is of particular importance because it adds new aspects to questions

  6. Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells

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    Krautkrämer Ellen

    2012-11-01

    Full Text Available Abstract Background Epithelio- and endotheliotropic viruses often exert polarized entry and release that may be responsible for viral spread and dissemination. Hantaviruses, mostly rodent-borne members of the Bunyaviridae family infect epithelial and endothelial cells of different organs leading to organ dysfunction or even failure. Endothelial and renal epithelial cells belong to the target cells of Old World hantavirus. Therefore, we examined the release of hantaviruses in several renal epithelial cell culture models. We used Vero cells that are commonly used in hantavirus studies and primary human renal epithelial cells (HREpC. In addition, we analyzed MDCKII cells, an epithelial cell line of a dog kidney, which represents a widely accepted in vitro model of polarized monolayers for their permissiveness for hantavirus infection. Results Vero C1008 and primary HREpCs were grown on porous-support filter inserts for polarization. Monolayers were infected with hantavirus Hantaan (HTNV and Puumala (PUUV virus. Supernatants from the apical and basolateral chamber of infected cells were analyzed for the presence of infectious particles by re-infection of Vero cells. Viral antigen and infectious particles of HTNV and PUUV were exclusively detected in supernatants collected from the apical chamber of infected Vero C1008 cells and HREpCs. MDCKII cells were permissive for hantavirus infection and polarized MDCKII cells released infectious hantaviral particles from the apical surface corresponding to the results of Vero and primary human epithelial cells. Conclusions Pathogenic Old World hantaviruses are released from the apical surface of different polarized renal epithelial cells. We characterized MDCKII cells as a suitable polarized cell culture model for hantavirus infection studies.

  7. Cytokine expression during early and late phase of acute Puumala hantavirus infection

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    Sadeghi Mahmoud

    2011-11-01

    Full Text Available Abstract Background Hantaviruses of the family Bunyaviridae are emerging zoonotic pathogens which cause hemorrhagic fever with renal syndrome (HFRS in the Old World and hantavirus pulmonary syndrome (HPS in the New World. An immune-mediated pathogenesis is discussed for both syndromes. The aim of our study was to investigate cytokine expression during the course of acute Puumala hantavirus infection. Results We retrospectively studied 64 patients hospitalised with acute Puumala hantavirus infection in 2010 during a hantavirus epidemic in Germany. Hantavirus infection was confirmed by positive anti-hantavirus IgG/IgM. Cytokine expression of IL-2, IL-5, IL-6, IL-8, IL-10, IFN-γ, TNF-α and TGF-β1 was analysed by ELISA during the early and late phase of acute hantavirus infection (average 6 and 12 days after onset of symptoms, respectively. A detailed description of the demographic and clinical presentation of severe hantavirus infection requiring hospitalization during the 2010 hantavirus epidemic in Germany is given. Acute hantavirus infection was characterized by significantly elevated levels of IL-2, IL-6, IL-8, TGF-β1 and TNF-α in both early and late phase compared to healthy controls. From early to late phase of disease, IL-6, IL-10 and TNF-α significantly decreased whereas TGF-β1 levels increased. Disease severity characterized by elevated creatinine and low platelet counts was correlated with high pro-inflammatory IL-6 and TNF-α but low immunosuppressive TGF-β1 levels and vice versa . Conclusion High expression of cytokines activating T-lymphocytes, monocytes and macrophages in the early phase of disease supports the hypothesis of an immune-mediated pathogenesis. In the late phase of disease, immunosuppressive TGF-β1 level increase significantly. We suggest that delayed induction of a protective immune mechanism to downregulate a massive early pro-inflammatory immune response might contribute to the pathologies characteristic of

  8. Pathogenicity and immunogenicity of the reassortant attenuated strain R566 of Rift Valley fever virus in sheep

    International Nuclear Information System (INIS)

    Full text: Rift Valley Fever (RVF) is an emerging zoonosis producing exploding outbreaks in ruminants, and humans cases, in Africa, Madagascar and the Arabic Peninsula. The Rift Valley Fever Virus (RVFV) is a Phlebovirus which possesses a single strand RNA genome composed of 3 separate segments, allowing the generation of reassortants by exchange of segments between parental strains. Here we show that the reassortant strain R566, selected to accumulate attenuation markers on all 3 segments, is both immunogenic and non-pathogenic after experimental inoculation to Sahelian sheep. Increasing doses of the R566 strain, ranging from 101 to 106 plaque forming units (pfu) were administered subcutaneously to six groups of four animals kept in a mosquitoes-proof facility in Senegal. As low as 104, and higher doses, was enough to induce RVFV neutralizing antibodies (Nab) titers higher than 1/10 in all four animals. These Nab became detectable 21 days after inoculation, whereas no clinical signs were detected with any of these doses. Three non-inoculated animals kept in contact as sentinels remained seronegative during the all observation period, indicating that seroconversion was the result of R566 inoculations. In order to examine the pathogenicity of the R566 strain, the minimal dose inducing Nab, i.e. 104, was inoculated to seven pregnant ewes during the first, second, third and fifth month of gestation. This vaccine regimen was immunogenic, non pathogenic, and non abortogenic in all 7 animals. All the pregnant ewes which received the R566 attenuated strain delivered healthy lambs and developed RVFV Nab titres>1/10, three weeks after inoculation. Nab titers decreased progressively below 1/10 after an average period of 120 days. All three lambs born from ewes inoculated at the fourth and fifth month of gestation quickly acquired colostral antibodies. Our study highlight the safety and immunogenicity of the live attenuated strain R566. These properties make R566 a promising

  9. Emerging arboviruses and public health challenges in Brazil.

    Science.gov (United States)

    Lima-Camara, Tamara Nunes

    2016-06-27

    Environmental modification by anthropogenic actions, disordered urban growth, globalization of international exchange and climate change are some factors that help the emergence and dissemination of human infectious diseases transmitted by vectors. This review discusses the recent entry of three arboviruses in Brazil: Chikungunya, West Nile, and Zika virus, focusing on the challenges for the Country's public health. The Brazilian population is exposed to infections caused by these three arboviruses widely distributed on the national territory and associated with humans. Without effective vaccine and specific treatment, the maintainance and integration of a continuos entomological and epidemiological surveillance are important so we can set methods to control and prevent these arboviruses in the Country. RESUMO A modificação do ambiente por ações antrópicas, o crescimento urbano desordenado, o processo de globalização do intercâmbio internacional e as mudanças climáticas são alguns fatores que vêm facilitando a emergência e disseminação de doenças infecciosas humanas transmitidas por vetores. Este comentário aborda a recente entrada de três arbovírus no Brasil, Chikungunya (CHIKV), West Nile (WNV) e Zika (ZIKV), com enfoque nos desafios para a Saúde Pública do País. Transmitidos por mosquitos vetores amplamente distribuídos no território nacional e associados ao homem, a população brasileira encontra-se exposta à infecção por esses três arbovírus. Na ausência de vacina eficaz e tratamento específico, são importantes a manutenção e integração de uma vigilância entomológica e epidemiológica contínua, a fim de direcionarmos métodos de controle e prevenção contra essas arboviroses no País. PMID:27355468

  10. Epidemiology of human papillomavirus infections: new options for cervical cancer prevention Epidemiología de las infecciones por el papilomavirus humano: nuevas opciones para la prevención del cáncer cervical

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    F. Xavier Bosch

    2003-01-01

    Full Text Available In the last two decades, the cervical cancer puzzle has become a coherent description that includes the identification of human papillomavirus (HPV as the sexually transmitted etiologic agent and the characterization of the major determinants of HPV acquisition. Triage studies have consistently shown that HPV testing is more sensitive that repeated cytology in identifying underlying high-grade lesions in women with atypical scamous cells of undetermined significance (ASCUS. Studies that reflect primary screening conditions have shown that the sensitivity of HPV tests is higher than standard cytology in detecting high-grade lesions whereas the specificity is similar only in women aged 30-35 and above. HPV vaccines have an intrinsic attraction as a preventive strategy in populations with limited resources. However, vaccines designed to widespread use are still in development and testing phases. Time is ripe for exploring in depth the clinical implications of current achievements and to devise novel strategies for the prevention of cervical cancer.En las ultimas dos décadas, el enigma del cáncer cervical (CaCu ha comenzado a ser dilucidado y actualmente se ha identificado a la infección por virus de papiloma humano (VPH como su agente etiológico transmitido sexualmente, y se han caracterizado los principales determinantes de infección por VPH. Estudios epidemiológicos han mostrado consistentemente que las pruebas de determinación de ADN de VPH son más sensibles que la citología repetida para la identificación de lesiones de alto grado en mujeres con diagnóstico de células escamosas atípicas de significado indeterminado (ASCUS. Diversos estudios que evalúan el tamizaje primario en CaCu, han mostrado que la sensibilidad de las pruebas de VPH es más alta que la citología estándar para detectar lesiones de alto grado, donde la especificidad es similar sólo en mujeres con edades entre 30 y 35 años o mayores. Las vacunas de VPH tienen

  11. Ocorrência generalizada do Lettuce mottle virus em três regiões produtoras de alface comercial do Estado de São Paulo Occurrence of Lettuce mottle virus on three lettuce producing areas from São Paulo State

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    Renate Krause-Sakate

    2008-02-01

    Full Text Available Os sequivírus são vírus isométricos transmitidos por afídeos. Lettuce mottle virus (LeMoV, um provável sequivirus foi descrito no Brasil em 1982 e causa sintomas de mosaico semelhantes aos observados pelo Lettuce mosaic virus (LMV. Um levantamento para ocorrência do LeMoV nos campos de produção de alface de três diferentes regiões do Estado de São Paulo (Mogi das Cruzes, Campinas e Bauru foi realizado durante 2002 a 2005. RNA total foi extraído e utilizado na detecção, em RT-PCR, com oligonucleotídeos específicos para o LeMoV. Do total de 1362 amostras, 137 (10,05% foram positivas para o LeMoV. Infecção mista com o LMV foi verificada em 43 amostras (31,4%. Foi verificada a ocorrência do LeMoV nas três diferentes regiões analisadas, porém sua ocorrência foi baixa nas diferentes épocas do ano.Sequiviruses are isometric aphid-borne plant viruses. Lettuce mottle virus (LeMoV, a putative sequivirus was first described in Brazil on 1982 causing similar mosaic symptoms as Lettuce mosaic virus (LMV. A survey for the occurrence of LeMoV on open field conditions was carried out during 2002 to 2005 on Mogi das Cruzes, Campinas and Bauru in São Paulo state. Total RNA was extracted and used on RT-PCR with specific LeMoV primers. On 1362 samples tested, 137 (10,05% were positive for LeMoV. Mixed infections with LMV was observed on 43 samples (31,4%. The presence of LeMoV was observed in the three different regions, but with low incidence during the year.

  12. Microorganismos indicadores de la calidad del agua potable en cuba

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    Marlen Robert Pullés

    2014-01-01

    Full Text Available Los agentes patógenos transmitidos por el agua constituyen un problema mundial que demanda un urgente control. Las bacterias, virus y parásitos causan enfermedades que varían en severidad. La determinación de microorganismos en el agua de consumo y su concentración proporcionan herramientas de control, indispensables para la toma de decisiones. Los controles rutinarios de todos los microorganismos, potencialmente riesgosos para la salud, resultan difíciles de llevar a cabo, debido a que ello representa, varios días de análisis y costos elevados. Para hacer una evaluación sencilla, económica y fiable de la presencia de patógenos, la vigilancia de la calidad del agua se efectúa mediante indicadores de contaminación, aplicando diferentes enfoques técnicos, cada uno con sus cualidades, defectos y limitaciones. El objetivo de esta revisión fue analizar el enfoque actual existente a nivel nacional e internacional, en relación con los indicadores microbiológicos empleados para la evaluación del agua potable, como elementos clave, y a partir de estos, proponer un esquema de monitoreo en Cuba. Los resultados reflejaron, la alternativa de considerar, la aplicación de un esquema para monitoreo complementario en Cuba, que incluya como indicadores de contaminación del agua potable además de las bacterias, algunos agentes biológicos no considerados en la norma, como los virus y los parásitos. Asimismo indicaron la necesidad de establecer un valor de referencia y definir los microorganismos a emplear en los monitoreos de validación, operativo o verificación. Esta propuesta aportaría importante información para la actualización de la norma cubana sobre la base del conocimiento de los estándares internacionales más reconocidos.

  13. MARTIAL ARTS TRAINING AND LEVELS OF ATTITUDE TOWARDS VIOLENCE IN ADOLESCENTS PRÁCTICA DE ARTES MARCIALES Y NIVELES DE ACTITUD HACIA LA VIOLENCIA EN ADOLESCENTES

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    Carlos Balsalobre-Fernández

    2011-03-01

    Full Text Available AbstractTraditionally, japanesse martial arts had, as well as technical and tactical lessons, discourses of peace and non-violence. Therefore, this study wants to prove whether the practitioners of these martial arts declare lower levels of attitude toward violence than non-practitioners. To this end, we administered he Brief Scale of Violence in Adolescents (Tejero-González, Balsalobre-Fernández and Ibanez-Cano, in press to two groups of boys and girls from 12 to 17 years of age: one group of judo, karate or jiujitsu (n = 57, and a control one (n = 57. Statistical analysis confirmed that martial arts practitioners declare significantly lower leves than the control group, both in general violence as gratuitous violence, but were not in violence linked to self-protection. Finally, we discuss the influence that factors such as years of practice or competitive orientation may have on the ability of the japanese martial arts to reduce levels of violence.Keywords: martial arts, judo, karate, jiujitsu, violence, adolescents.ResumenTradicionalmente, las artes marciales japonesas han transmitido de forma inseparable a sus enseñanzas técnico-tácticas un discurso de paz y rechazo de la violencia. Por ello, este estudio trata de comprobar si los practicantes de dichas artes marciales declaran menores niveles de actitud hacia la violencia que los no practicantes. Para tal fin, se administró la Escala Breve de Violencia en Adolescentes (Tejero-González, Balsalobre-Fernández e Ibáñez-Cano, en prensa a dos grupos de chicos y chicas de 12 a 17 años de edad: uno formado por practicantes de judo, karate  o jiujitsu (n=57, y otro control (n=57. El análisis estadístico confirma que los practicantes de artes marciales declaran niveles significativamente menores que los del grupo control, tanto en violencia general como en violencia gratuita, no siendo así en violencia vinculada a la autoprotección. Finalmente, se discute la influencia que algunos

  14. Educação e serviços de saúde: avaliando a capacitação dos profissionais Education and health services: evaluating professional training

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    Solange L'Abbate

    1999-01-01

    Full Text Available Análisa-se os resultados de pesquisa de acompanhamento e avaliação do Curso de Especialização em Saúde Pública realizado pelo Departamento de Medicina Preventiva e Social da Faculdade de Ciências Médicas da UNICAMP, turmas de 1993 e 1995. A investigação foi realizada durante o curso e alguns meses depois do término do mesmo. Os alunos informaram suas atividades profissionais, as dificuldades e facilidades em desenvolvê-las e o compromisso com os serviços públicos de saúde nos quais trabalhavam; informaram também sobre suas expectativas em relação à especialização em saúde pública. Ao final das disciplinas, os alunos avaliaram a relevância dos conteúdos transmitidos, sua compreensão em relação aos mesmos e os erros e acertos das estratégias didáticas utilizadas. Transcorridos seis e oito meses do final do curso, os profissionais avaliaram a aplicação do que haviam aprendido. Instrumentos de levantamento de dados, tais como, dinâmicas de grupo, questionários individuais e grupos focais, compuseram uma abordagem metodológica, mediante a qual uma mesma clientela foi investigada a respeito de uma experiência que estava sendo vivenciada.This paper analyzes the results of a follow-up study and evaluation of the Specialization Course in Public Health administered from 1993 to 1995 by the Department of Preventive and Social Medicine at the School of Medical Sciences, Universidade de Campinas, Brazil. The study was conducted during the course and with a follow-up several months later. Students reported on their professional activities, difficulties and facilities in performing them, and their commitment to the public health services in which they worked. They also expounded on their needs and expectations regarding specialized training in public health. At the end of the required course load (first semester and the optional courses (second semester, students evaluated the course content's relevance, their own

  15. Miocardiopatía Chagásica con taquicardia ventricular: Hospital Obrero Nº 2 “Caja Nacional de Salud”

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    Dr. Raúl Arnez Terrazas

    2012-06-01

    Full Text Available La miocardiopatía chagásica es la inflamación del músculo cardiaco producida por el Trypanosoma cruzi. Los parásitos que se encuentran en los excrementos de la vinchuca (Tripanosoma infestans son transmitidos al organismo luego de la alimentación del vector. Afecta varias áreas del organismo humano como son los nervios periféricos y las paredes del intestino grueso entre otros, sin embargo el corazón es uno de los órganos que con mayor frecuencia es afectado una vez producida la infección. Otros mecanismos de infección son a través de transfusiones de sangre contaminada y por medio de la placenta al feto. Se describe el caso de un paciente de 53 años transferido del policlínico Nº 32 de la ciudad de Cochabamba con el diagnóstico de Insuficiencia Cardiaca Congestiva y bradicardia sinusal: El diagnóstico de bradicardia sinusal se realizó hace 2 años, donde se le propuso al paciente un implante de marcapasos, que este no realizó. Ingresó a salas de unidad de terapia intensiva con el diagnóstico de admisión de síndrome de taquicardia – bradicardia con antecedente de miocardiopatía chagásica. Al examen físico paciente presenta disnea, mareos y palpitaciones. Durante su internación el paciente presenta episodios de taquicardia sinusal de hasta 150 latidos por minuto y bradicardias sistólicas hasta 30 latidos por minuto, taquicardia ventricular y fibrilación auricular por lo que se considera como diagnóstico: miocardiopatía chagásica con taquicardia ventricular. Se decide implantar un marcapaso temporal y luego definitivo, se decide descontinuar la dosis de amiodarona, a la auscultación se presenta ritmo regular sin ruidos sobre agregados.

  16. Condiciones para la transmision del hantavirus en zona andina de Río negro, Argentina

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    Gabriel Talmon

    2014-10-01

    Full Text Available El Síndrome Pulmonar por Hantavirus (SPH es una enfermedad de etiología viral que causa en el hombre un cuadro respiratorio grave. En Patagonia, la enfermedad es causada por el virus Andes Sur (AND, transmitido por el roedor Oligoryzomys longicaudatus. El objetivo del presente trabajo fue identificar las actividades del hombre que favorecen su exposición a roedores, denominados escenarios de contagio. Se realizó un estudio retrospectivo a partir de información recolectada en investigaciones de casos ocurridos en Río Negro, mediante Fichas Clínico-Epidemiológicas e informes de evaluación ecológico/ambiental. Se definieron como variables a ser consideradas: edad, sexo, época del año, grado de urbanización, localización geográfica, integración del hombre al hábitat de roedores, fuente probable de exposición, actividad humana y nivel de saneamiento. Se estudiaron 32 casos. La exposición rural se verificó en 18 (56.2% de los casos y 10 (31.3% en paraje rural (grupo de viviendas en zona rural. En relación al ambiente antropogénico 24 (75% resultaron en ambientes modificados por el hombre y 8 (25% en áreas poco modificadas. El sitio de exposición de mayor importancia en El Bolsón fue el interior de edificaciones en 8 de los 18 casos allí registrados (44.5%, mientras que en Bariloche fueron ambientes de exterior con 8/14 (57.1% casos. La actividad de riesgo fue laboral en 23 (71.9% de los casos y recreacional en 7 (28.1%. Determinar los escenarios de contagio a nivel local ha aportado información para aplicar todos los recursos disponibles en materia de prevención y educación sanitaria.

  17. Efecto del extracto de paico (chenopodium ambrosioides, en parásitos gastrointestinales de gallos de pelea (gallus domesticus.

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    Paola Rodríguez

    2011-10-01

    Full Text Available El uso empírico de las plantas como agentes de salud es ampliamente conocido en múltiplesculturas del mundo, conocimientos que son transmitidos de generación en generación. En la actualidad, las plantas medicinales y sus extractos, son utilizados con fines terapéuticos, instaurándose como una alternativa farmacológica, para diferentes patologías tanto en humanos como en animales. En la etnomedicina de algunas regiones de Latinoamérica, el paico (Chenopodium ambrosioides ha sido empleado en infusión de hojas y flores comocarminativo y digestivo, pero principalmente como antihelmíntico. Por lo tanto, el presenteestudio se realizó con el fin de evaluar el efecto antiparasitario del extracto de esta planta engallos de pelea (Gallus domesticus en un criadero de la ciudad de Tunja-Boyacá, ya que los propietarios de estas aves lo utilizan de manera artesanal como desparasitante natural. Para el trabajo se utilizaron 45 ejemplares, organizados en 3 grupos a los cuales, cada 15días y durante un mes, se les administró, por vía oral, 0,1 ml/ Kg de extracto de paico (grupoT2, 0.5ml/Kg de un medicamento comercial a base de Levamisol-Ivermectina (grupo T3 y0.5ml/ave de agua (grupo T1 control. Se recolectaron muestras de materia fecal con el objeto de determinar la presencia de huevos y/o larvas de parásitos, utilizando la técnica de Formol-Eter. Durante el estudio fueron identificados diferentes especies de ascaridia spp, heterakis gallinarum, eimeria sp, huevos y larvas de trichostrongylus. Posterior a la administracióndel tratamiento con extracto de paico se encontraron huevos de ascaridia galli, heterakis gallinarum, eimeria sp , y al suministrar la última dosis sólo se identificaron huevos de ascaridia galli e eimeria sp , lo que corrobora su efecto antiparasitario.

  18. Evaluation of the possible role of ants (Hymenoptera: Formicidae as mechanical vectors of nematodes and protists

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    Fabio Villani

    2008-10-01

    Full Text Available Nematodes and protists can be transmitted to humans in many ways and little concern has been given to the mechanical transmission by ants. This study aimed at analysing how the eggs of Ascaris lumbricoides and cysts of Entamoeba coli could be mechanically transmitted to the man by Formicidae. Through the experiments using nests of Tapinoma melanocephalum, Linepithema humile and Monomorium pharaonis reared in the laboratory allied to observations of some 17 ant species in an urban park area in Mogi das Cruzes (SP, it was found that L. humile was capable of carrying eggs of A. lumbricoides both in the field and laboratory conditions (1 worker, as well as was Camponotus rufipes (2, Solenopsis saevissima (1 and Acromyrmex niger (1. The cysts of Escherichia coli were found over three workers of C. rufipes. Although the frequency of the workers found transporting pathogens was low, the capacity of common household species in carrying pathogens like nematodes and protists was demonstrated.Os Nematoda e Protista podem ser transmitidos ao homem de diversas maneiras, mas pouca ênfase é dada para a transmissão mecânica por intermédio de formigas. Assim, esse trabalho procurou investigar a transmissão mecânica de ovos de Ascaris lumbricoides e cistos de Entamoeba coli pelos Formicidae. Através de experimentos com espécies mantidas em ninhos no laboratório (Tapinoma melanocephalum, Linepithema humile e Monomorium pharaonis e com 17 espécies de formigas de uma área antropizada na região de Mogi as Cruzes (SP, foi possível constar que os ovos A. lumbricoides foram transportados por L. humile, tanto no campo (1 operária como no laboratório (1 operária, por Camponotus rufipes (2, por Solenopsis saevissima (1 e por Acromyrmrex niger (1. Em três operárias de C. rufipes foram encontrados cistos de E. coli. Apesar da baixa incidência de transporte, as três primeiras espécies pelo fato de viverem muito próximas ao ser humano, podem levar para

  19. ENSEÑANZA DE LA HISTORIA: ¿PROBLEMA METODOLÓGICO O PROBLEMA CONCEPTUAL?

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    Luis Alberto Buttó

    2013-08-01

    ógicas escogidas como herramientas de instrumentación del proceso enseñanza-aprendizaje y apuntar a dilucidar con claridad el objetivo general perseguido con la enseñanza de la historia y el tipo de historia transmitido en los segmentos mencionados de la educación formal venezolana, asumiendo que el primer elemento -objetivo- condiciona al segundo: tipo de historia enseñada.

  20. Overdressed and Underexposed or Underdressed and Overexposed?

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    Lori G. Beaman

    2013-12-01

    Full Text Available Judges and public policy makers have transmitted conflicting messages in relation to women’s bodies and have made normative judgements about how women are to appear in public. Women who have been judged to be wearing too much are called to undress as they are seen as interfering with the rights of others or being oppressed. Women judged to be wearing too little are urged to clothe themselves to avoid being seen as inviting sexual assault or dressing like always sexually available prostitutes. Juxtaposing these two situations together, the oddness of judicial and public regulation of women’s clothing becomes more starkly exposed. This paper examines the shifting nature of equality discourse and the naming of women’s oppression; the near-disappearance of patriarchy as an explanatory framework; and the quagmire of women’s agency. The concluding section proposes shifting the focus from differences between women's experiences to similarity in order to facilitate critical inquiry, dialogue and strategic action that might re-constitute women’s equality in new ways. Los jueces y los responsables políticos han transmitido mensajes contradictorios en relación a las mujeres y han hecho juicios normativos acerca de cómo las mujeres deben aparecer en público. Las mujeres que han sido criticadas por vestir en exceso son llamadas a desvestirse ya que son vistas como una interferencia en los derechos de otros o como oprimidas. Las mujeres juzgadas por vestir mínimamente, por su parte, son llamadas a vestirse para evitar ser vistas como una invitación al asalto sexual o como prostitutas siempre sexualmente disponibles. Yuxtaponiendo estas dos situaciones a la vez, la extravagancia de la regulación judicial y pública de la vestimenta de las mujeres se expone más crudamente. Este artículo examina la naturaleza cambiante del discurso de igualdad y el nombramiento de la opresión de las mujeres, la casi desaparición del patriarcado como un marco

  1. Detección y cuantificación del Potato mop-top virus (PMTV en Colombia mediante qRT-PCR

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    Nevar García Bastidas

    2013-04-01

    Full Text Available El Potato mop-top virus (PMTV es uno de los virus re-emergentes en cultivos de papa en Colombia. Es transmitido por Spongospora subterranea, el agente causal de la sarna polvosa. La detección del PMTV presenta dificultades debido a su distribución irregular en las plantas, bajo título y movimiento sistémico como ARN desnudo. Con el fin de ampliar el rango de herramientas disponibles para detectar el PMTV en los programas de certificación de tubérculo-semilla, en este estudio se evaluó la prueba de RT-PCR en tiempo real (qRT-PCR en dos pasos: con los cebadores PMTV-1948F/PMTV-2017R y la sonda Taqman® PMTV-1970, dirigidos al gen CP-RT del ARN2 viral. Se construyó una curva estándar a partir de la transcripción in vitro de un fragmento de 1513 pb de este gen. Posteriormente, se evaluó la utilidad de la técnica a partir de tres tipos de muestras: plantas señuelo de Nicotiana benthamiana y Solanum phureja inoculadas con quistosoros de Sss, raíces de papa con síntomas de sarna polvosa del municipio de La Unión (Antioquia y tubérculos-semilla. Mediante qRT-PCR fue posible detectar el virus en 11 de las 20 muestras de raíz de plantas señuelo, mientras que 14 de las 15 muestras de raíces de papa resultaron positivas, estimándose una concentración entre 4.72 x 10(11 y 7.60 x 10(13 partículas virales/µl. Adicionalmente, en el ensayo de tubérculo-semilla se determinó la presencia del PMTV en una de las 16 muestras. Estos resultados indican la viabilidad de utilizar rutinariamente la técnica de qRT-PCR para la detección de PMTV en Colombia.

  2. Riesgo de transmisión de Leishmania (Kinetoplastida: Trypanosomatidae en Mérida Venezuela

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    Elsa Nieves

    2014-09-01

    Full Text Available La leishmaniasis es una enfermedad causada por la infección de un parásito protozoario del género Leishmania, transmitido por la picada de insectos hematófagos conocidos como flebotominos. El estudio tiene como objetivo determinar la presencia de flebotominos en los Distritos Sanitarios del estado Mérida y diseñar un mapa de riesgo de transmisión entomológico. Se utilizaron cuatro métodos de captura de flebotominos, los ejemplares se identificaron y se les determinó la infección natural por Leishmania. Se estimó la riqueza de especies, y se realizó un proceso analítico Jerárquico. Los resultados muestran la presencia de diversas especies de flebotominos en los Distritos Sanitarios del estado Mérida, siendo las especies de mayor frecuencia L. youngi, L. gomezi, L. ovallesi y L. walkeri. Se detectó 2,1% de infección natural con Leishmania, la cual se encontró en las 4 especies más frecuentes. Se presenta un mapa de riesgo de transmisión entomológico para el estado Mérida. El conocimiento de la situación actual de los vectores de Leishmania en el estado Mérida y el riesgo de transmisión son relevantes a la hora de considerar la prevención y posible surgimiento de nuevos brotes de leishmaniasis. Abstract (english The leishmaniasis is a disease caused by infection with a protozoan parasite of the genus Leishmania, transmitted by the bite of blood-sucking insects known as sandflies. The study aims to determine the presence of sandflies in Merida state health districts and design a map of entomological risk of transmission. Four methods capture sandflies were used, the specimens were identified and natural Leishmania infection was determined. The richness species was estimated and analityc Hierarchie procesess was performed. The results show the presence of various species of sandflies in Merida state health districts, L. youngi, L. gomezi, L. ovallesi and L. walkeri were most abundant species. The 2.1% of natural infection

  3. Atitudes de estudantes universitários frente ao consumo de materiais pornográficos University students' attitudes towards pornographic material consumption

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    Valeschka Martins Guerra

    2004-08-01

    Full Text Available A expansão do mercado de produtos pornográficos traz à tona a discussão acerca da influência dos valores transmitidos pela mídia no comportamento dos indivíduos. A questão da pornografia permanece sem o devido aprofundamento, pois o Brasil ainda é carente de pesquisas nesta área. Este estudo descreve a elaboração e validação de um instrumento de medida das atitudes frente ao consumo de materiais pornográficos, aplicada a 336 universitários e os dados submetidos à análise fatorial. Os 33 itens construídos confirmaram a existência de duas dimensões, que apresentam níveis de precisão adequados: a primeira, denominada "Efeitos positivos da pornografia", em que a expressão do sexo neste tipo de material é tida como favorável à vida sexual (alfa = 0,94; na segunda dimensão, "Efeitos nocivos da pornografia", esta expressão é percebida como prejudicial aos relacionamentos íntimos, associada ao vício e ao isolamento (alfa = 0,85.The expansion of the pornographic material market raises the question of the influence of the values transmitted by the media in people's behavior. Despite the apparent interest in sexuality by the society in general, Brazil lacks studies on this topic, which has not yet been deeply discussed. This study describes the elaboration and validation of a psychometric instrument for the measuring of attitudes towards the consumption of pornographic material, answered by 336 university students and submitted to a factor analysis. 33 items were built in order to cover two dimensions, presenting adequate reliability scores: (1 "Positive effects of pornography", expressing the idea that pornographic material is favorable to sexual life (alpha = 0.94 and (2 "Harmful effects of pornography", which expresses the idea that this same material is noxious to intimate relationships and it is associated to vice and isolation (alpha = 0.85.

  4. Ocorrência de raiva em ovinos no Rio Grande do Sul Occurrence of rabies in sheep in Rio Grande do Sul, Brazil

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    Daniel R. Rissi

    2008-10-01

    Full Text Available Descreve-se a ocorrência de raiva em ovinos na região Central do Rio Grande do Sul em novembro de 2003. Foram afetados dois ovinos de raça mista, um macho de três meses e uma fêmea de 2,5 anos de idade que apresentaram sinais clínicos com evolução de cinco dias e caracterizados por dificuldade de locomoção, tremores musculares, decúbito lateral, convulsões, opistótono e febre. Histologicamente havia mielomeningoencefalite não-supurativa, associada a inclusões eosinofílicas intracitoplasmáticas (corpúsculos de Negri em neurônios nos dois ovinos afetados. Em um ovino em que o gânglio de Gasser foi examinado, havia ganglionite não-supurativa. As lesões concentravam-se predominantemente na substância cinzenta da medula espinhal, no tronco encefálico e no cerebelo. Antígeno viral foi detectado em seções selecionadas de ponte e bulbo submetidas ao teste de imuno-histoquímica utilizando anticorpo policlonal anti-ribonucleoproteína do vírus da raiva. Os casos ocorreram em meio a um surto de raiva bovina transmitida por morcegos e foram considerados, com bases epidemiológicas, como transmitidos da mesma forma, como ocorre na raiva endêmica de bovinos no Rio Grande do Sul.Cases of rabies in sheep occurring in November 2003, in central Rio Grande do Sul state, Brazil, are described. A 3-month-old male, and a 2.5-month-old female sheep were affected. Clinical signs were characterized by abnormal gait, trembling, lateral recumbency, convulsion, opisthotonus, and fever. Histological findings included a non-suppurative myelomeningoencephalitis associated with intracytoplasmic eosinophilic inclusion (Negri bodies in neurons. Lesions were predominantly observed in gray matter of the spinal cord, brainstem and cerebellum. There was non-suppurative Gasserian ganglionitis in one sheep in which this structure was examined. Immunohistochemistry using rabies virus ribonucleoprotein polyclonal antibody yelded positive result in brain

  5. FÉ E PRAGMATISMO NO SERTÃO

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    Emilio Tarlis Mendes Pontes

    2014-01-01

    Full Text Available El imaginario de los “campesinos” es muy impregnado por figuras místico-míticas que tienen una profunda influencia en la formación de su ethos social. Folclorificadas o reales, hacen influencia individual y socialmente - aunque no necesariamente consciente de ese poder – en una miríada de “devotos”, en el sentido lato, en el cotidiano representativo de su tierra/identidad: el “campo”. Esta participación se entrelaza entre diversas corrientes: eclesial, cultural, religioso, transcendental, etc, donde no siempre hacen un dialogo conceptual, fenomenal o institucional o no están de acuerdo y luchan cuando plantean cuestiones de los dominios de territorialidad religiosas, cuyos lideres y/o portadores de “vínculos sobrenaturales” dictan lo que seguir. Esta influencia es personal o de forma remota, transmitido de generación en generación, de manera inculturada o autóctonas y tiene su origen en la Heteronomía, que forjó el imaginario religioso de lo “campesino”. El poder de persuasión es explorado en varias áreas desde la política a la religión, del académico al social, del individuo a las masas. Ha sido canalizado en el trabajo social como para influir en las campañas y mítines electorales; en la identidad del “campesino” a una cultura líquida; en la solidificación del analfabetismo político en la educación contextualizada en regiones semiáridas; una fe esclarecida a una práctica religiosa irracional e irresponsable. Este trabajo tiene como objetivo discutir la íntima relación entre estos fenómenos citados manifiesto en cálidas demostraciones públicas o en acciones individuales, que constituyen la esencia del “campesino” y sus alrededores.

  6. Caracterização parcial de um Tymovirus isolado de tomateiros

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    Isabel Cristina Batista

    2012-01-01

    Full Text Available Um Tymovirus isolado de sementes de tomateiro (Solanum lycopersicum 'Paronset' com sintomas de necrose, denominado TyToRS08, foi caracterizado quanto ao círculo de hospedeiras, propriedades físico-químicas, sorológicas e moleculares. O vírus foi transmitido mecanicamente a partir de folhas, frutos e sementes infectadas. A gama de hospedeiras incluiu espécies de Amaranthaceae, Chenopodiaceae e Solanaceae, induzindo sintomas local e sistêmicos na maior parte das espécies, exceto para Nicotiana sylvestris, N. tabacum 'Samsun', 'White Burley' e 'Xanthi' que não manifestaram sintomas. Em tomateiros 'Alambra', 'Thomas', 'Diva', 'Romana' e 'Graziela', o vírus induziu sintomas de mosaico e necrose branca. A presença do vírus foi detectada, nas sementes infectadas de todas as cultivares avaliadas, porém não se notou em plântulas após a germinação destas sementes. Foi obtida uma preparação purificada com concentração de 1,04 mg mL-1, empregada na produção de um antissoro policlonal que reagiu em PTA-ELISA com um título de 1/32768. Utilizou-se um par de oligonucleotídeos degenerados, desenhados para anelar na ORF 1 de espécies de Tymovirus, que permitiu a amplificação por RT-PCR de fragmentos com cerca de 700 pb. Após o alinhamento e a análise das sequências, verificou-se que o isolado TyToRS08 possuía valores abaixo de 70% de identidade com as espécies de Tymovirus, indicando que pode se tratar de uma espécie distinta das descritas no gênero, com potencial epidemiológico, pela sua estabilidade e disseminação por sementes de tomate.

  7. Detection, transmission, pathogenicity and chemical treatment of fungi in Ceiba speciosa seeds Detecção, transmissão, patogenicidade e controle químico de fungos em sementes de paineira (Ceiba speciosa

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    Marília Lazarotto

    2010-06-01

    Full Text Available The aim of this work was to evaluate fungus association, transmission and pathogenicity, besides chemical seed treatment in Ceiba speciosa seeds from different regions of southern Brazil. Seven seed samples were used to do the germination test, fungus detection by blotter test and potato-dextrose-agar (PDA, fungus transmission and pathogenicity tests; besides, chemical seed treatments were tested. Germination ranged from 0 to 59,5%. The following fungi were associated in the seeds: Fusarium sp., Alternaria sp., Colletotrichum sp., Curvularia sp. and Pestalotia sp.; in addition, Fusarium sp. was found in all the samples. Alternaria sp. and Fusarium sp. were transmitted by seeds. The isolates of Alternaria sp., Colletotrichum sp. and Fusarium sp., were pathogenic to seedlings and seeds. The seed treatment with methyl tiophanate and the combination captan + methyl tiophanate reduced Fusarium sp. incidence.Neste trabalho avaliou-se a incidência, transmissão, patogenicidade de fungos e a eficiência do tratamento químico no controle dos mesmos em sementes de paineira (Ceiba speciosa oriundas de diferentes locais do sul do Brasil. Para tanto, utilizaram-se sete amostras de sementes, com as quais realizaram-se testes de germinação, sanidade empregando-se o método do papel-filtro (PF e de plaqueamento em batata-dextrose-ágar (BDA, transmissão e patogenicidade dos fungos e tratamento químico das sementes. A germinação variou de 0 a 59,5%. Foram detectados os seguintes fungos associados às sementes: Fusarium sp., Alternaria sp.,Colletotrichum sp., Curvularia sp. e Pestalotia sp.; sendo que Fusarium sp. foi encontrado em todas as amostras. Ambos os métodos de detecção, PF e BDA, foram eficientes na detecção da maior parte dos fungos. Alternaria sp. e Fusarium sp. foram transmitidos via sementes. Os isolados de Alternaria sp., Colletotrichum sp. e Fusarium sp. foram patogênicos às plântulas e os tratamentos das sementes com tiofanato met

  8. A gestão quotidiana da herança indesejada: um estudo sociológico sobre o risco genético de câncer hereditário = Quotidian management of an undesired inheritance: a sociological study on genetic risk of hereditary cancer

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    Felismina Rosa Parreira Mendes

    2006-01-01

    Full Text Available Neste artigo, apresentam-se alguns dos dados obtidos em um estudo qualitativo sobre o quotidiano dos sujeitos em risco genético de câncer hereditário do cólon. A perspectiva analítica, que é possível esboçar a partir da análise dos relatos desses indivíduos remete para a importância das condições-limite de aceitação positiva do risco e que, actualmente, se exprime na tendência de diferentes projectos tecnológicos – de que a genética é um exemplo – apelarem aos desejos individuais e colectivos de um futuro melhor. Outro dos traços estruturantes da gestão quotidiana do risco genético centra-se naexperiência familiar do câncer, que permite accionar práticas preventivas indispensáveis ao controlo da doença. Esse saber acumulado e transmitido de geração em geração, por vezes oralmente, outras vezes através da experiência quotidiana com o sofrimento e a morte, ou na partilha das alegrias inerentes ao triunfo sobre o câncer configura-se como um dos pilares na gestão quotidiana da herança, indesejada. This study presents some results obtained on a qualitative study about the everyday life of the individuals in genetic risk of hereditary cancer. The analytical perspective, which can be outlined on the basis of an analysis of the individuals’ reports, points out the importance of the limiting conditions of risk acceptance. This is presently expressed in the tendency for the different technological projects – including genetics – to appeal to both individual and collective wishes for a better future. Another conclusion is that the daily management of genetic risk is centred on the family experience of cancer, which allows preventives practices, indispensable to control the disease, to be put into action. This knowledge, sometimes accumulated and transmitted orally from generation to generation, and other times obtained from experiencing pain and death on a daily basis, or even by sharing in the joy of

  9. Criação e Disseminação de Conhecimento Local em Pequenas Empresas Familiares: O Caso da Região da Quarta Colônia de Imigração Italiana, no Rio Grande do Sul, Brasil

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    Alisson Eduardo Maehler

    2011-10-01

    Full Text Available O artigo constituiu-se em um estudo de múltiplos casos que envolveu a realização de entrevistas com proprietários de três cantinas artesanais de produção de vinho, localizadas na região da Quarta Colônia de Imigração Italiana, na região central do Estado do Rio Grande do Sul, Brasil. Buscou-se, nesse contexto, analisar quais as influências da localidade, da proximidade e das relações sociais nos processos de criação e transferência de conhecimento local. Os dados foram coletados através de realização de entrevistas semi-estruturadas com os proprietários de cantinas artesanais, que se situam no mesmo município, em uma mesma localidade, situadas poucos quilômetros umas das outras. Como resultados destacam-se que a proximidade geográfica, per si, não é condição suficiente para que o conhecimento seja criado, transmitido e mantido em organizações de uma dada localidade. Particularmente no caso de produtos artesanais, tais como o vinho, e que envolvem uma grande parcela de conhecimentos tácitos, o estabelecimento de relações sociais e familiares é condição necessária para que o conhecimento possa ser criado, compartilhado e preservado na localidade. Contudo, uma série de problemas vem sendo observados na localidade analisada e que fazem com que o conhecimento fique limitado a algumas famílias e não seja mantido ao longo do tempo. Por exemplo, o fato do conhecimento de produção vinícola ser essencialmente tácito e não codificado; o desinteresse das novas gerações com o conhecimento que se possui e as fracas interações e compartilhamento de conhecimento entre os vinicultores.DOI:10.5585/riae.v10i2.1735

  10. Mídia, violência e alteridade: um estudo de caso Media, violence and otherness: a case study

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    Fabiana Pinheiro Ramos

    2003-12-01

    Full Text Available O objetivo do presente trabalho é discutir algumas representações que circularam na mídia impressa a respeito do caso de um seqüestro de ônibus que ocorreu na cidade do Rio de Janeiro, em junho do ano 2000. O seqüestro foi transmitido ao vivo pela TV e teve um desfecho trágico, tendo recebido ampla cobertura nos meios de comunicação. Foram analisadas duas matérias de revistas semanais de circulação nacional e setenta matérias de um jornal de grande circulação em São Paulo e no resto do país. A discussão é centrada na figura do agente da violência - o criminoso, a partir da perspectiva da produção da alteridade. Os resultados apontam que a mídia, na maioria das vezes, contribui para a mitificação do fenômeno da violência e para a manutenção da concepção de que a violência é um problema individual de alguém que não sabe lidar com suas emoções e dificuldades, e que merece, portanto, ser excluído do convívio social.The objective of this paper is to discuss some representations that circulated on press media about the bus kidnapping case that occurred in the city of Rio de Janeiro, in June 2000. The kidnapping was aired live on TV with a big mass media coverage, and it had a tragic end. Our analysis comprised two reports of magazines of national circulation and seventy news reports of an important newspaper from São Paulo, read all over the country. The focus of the analysis is the figure of the agent of violence - the criminal, adopting the otherness production perspective. The results showed that media contribute to mystify the violence, and to sustain the conception that violence is an individual problem of someone who cannot deal with emotions and difficulties, and therefore deserves to be excluded from social ground.

  11. Concepto y Representación de la Violencia de Género: Reflexiones sobre el Impacto en la Población Joven (Concept and Representation of Gender-based Violence: Reflections about the Impact on Young People

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    Belén Zurbano Berenguer

    2015-06-01

    Full Text Available Recent studies and reports reveal that young people have a distorted concept of gender-based violence and their tolerance towards it does not decrease. There is a context of institutional concern about prevention and eradication of gender-based violence in Spain. In this context, this paper reflects on gender-based violence in order to compare the collective adolescent-young imaginary and social messages sent by the media on this problem.  We will analyze this concept based on theoretical feminist definitions, the legal national an international framework and data about the presentation of this problem on the media. We will conclude that gender-based violence is transmitted in a very simplistic and reductionist way, a way too far from feminist frameworks, so adolescents and young people find identifying and rejecting it difficult.  Los últimos estudios e informes revelan que la juventud tiene un concepto distorsionado de la violencia de género y sus niveles de tolerancia hacia las agresiones violentas por razón de género no disminuyen. En un contexto de preocupación institucional por la prevención y erradicación  de esta violencia, el presente trabajo reflexiona sobre el concepto de violencia de género con la finalidad de emprender una comparación entre el imaginario colectivo adolescente o joven y las conceptualizaciones y transmisiones sociales, en este caso por parte de los media, que se hacen de este problema. A partir de un estudio conceptual basado en las descripciones teóricas feministas de las violencias que sufren las mujeres, una revisión de los marcos legislativos definitorios del problema (tanto a nivel nacional como internacional y de datos sobre su representación en los medios de comunicación, se concluye que este problema es transmitido de modo simplista y reduccionista, muy alejado de los marcos de interpretación feministas, lo que dificulta su identificación y rechazo por parte de las personas jóvenes y

  12. Detecção, transmissão e patogenicidade de fungos em sementes de angico-vermelho (Parapiptadenia rigida Detection, transmission and pathogenicity of fungi on seeds of Parapiptadenia rigida ("angico-vermelho"

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    Caciara Gonzatto Maciel

    2012-12-01

    Full Text Available Angico-vermelho (Parapiptadenia rigida (Benth. Brenan é uma espécie nativa de grande valor ecológico e econômico, importante para a recomposição de áreas degradadas. O presente trabalho avaliou incidência, transmissão e patogenicidade de fungos associados a sementes de angico-vermelho de distintas procedências do Estado do Rio Grande do Sul. Para isso, utilizaram-se três amostras de sementes, com as quais realizaram-se testes de germinação, sanidade empregando-se o método do papel-filtro (PF e de plaqueamento em batata-dextrose-ágar (BDA, transmissão e patogenicidade dos fungos. A germinação das sementes de angico-vermelho variou de 63 a 91 %. Os fungos considerados potencialmente patogênicos encontrados associados as sementes de angico-vermelho foram: Alternaria sp.; Botrytis sp.; Fusarium sp.; Cladosporium sp. e Pestalotia sp.; sendo que Fusarium sp. foi detectado em todas as amostras pelo método PF, e foi transmitido via semente causando má formação do sistema radicular e dos cotilédones e tombamento de pré emergência. Sua patogenicidade foi confirmada."Angico-vermelho" (Parapiptadenia rigida (Benth. Brenan is a native species of great ecological and economic importance for the recovery of degraded areas. This study evaluated the incidence, the transmission and the pathogenicity of fungi associated with "angico-vermelho" seeds from different provenances of the state of Rio Grande do Sul. Thus, we used three samples of seeds, with which germination and sanity tests were carried out by adopting the methods of filter paper (FP, plating on potato dextrose agar (PDA, transmission and pathogenicity of fungi. Germination of "angico-vermelho" seeds ranged from 63-91%. The fungi considered potentially pathogenic and found associated with "angico-vermelho" seeds were: Alternaria sp.; Botrytis sp.; Fusarium sp.; Cladosporium sp. and Pestalotia sp.. Fusarium sp. was detected in all samples according to the FP method and was

  13. Begomovírus infectando a cultura de pimentão no estado de São Paulo Begomovirus infecting pepper crops in São Paulo state

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    Denise Nakada Nozaki

    2010-09-01

    Full Text Available Vírus do gênero Begomovirus são transmitidos por mosca-branca Bemisia tabaci G., e constituem um dos problemas fitossanitários sérios em diversas culturas. Plantas de pimentão coletadas em oito regiões do Estado de São Paulo, foram submetidas a extração de DNA total e PCR com primers universais e degenerados para begomovírus, que amplificam parte da região codificadora para a proteína capsicial. Os dados indicam a presença de begomovírus em pimentão nas cinco regiões coletadas. Análise das seqüências do DNA viral e análise filogenética revelaram identidade com dois begomovírus nativo da América. Tomato severe rugose virus - ToSRV (AY029750 e com Tomato yellow vein streak virus (ToYVSV, AY829113, espécies descritas infectando tomateiro no Brasil. A presença de begomovírus em pimentão foi verificada nas regiões de Alvinlândia, Ubirajara, Botucatu, Elias-Fausto, Paulínia, Mogi Guaçu, Paranapanema e Pirajú.Virus to the genus Begomovirus transmitted by whiteflies Bemisia tabaci G., and considered one of the most important fitossanitary problems for several cultures. Pepper plants were collected in eight the regions of São Paulo State, the extraction of total DNA and PCR were submitted with universal and degenerate primers was used to amplify partial sequences of the begomovírus coat protein gene. The data indicated the presence of begomovírus in five collected regions. DNA viral sequence analysis and phylogenetic analysis revealed identity with two native begomovírus of America. Tomato severe rugose virus - ToSRV (AY029750 and with Tomato yellow vein streak virus (ToYVSV, AY829113, described species infecting tomateiro in Brazil. The begomovírus presence in pepper plant was verified in the areas of Alvinlândia, Ubirajara, Botucatu, Elias-Fausto, Paulínia, Mogi Guaçu, Paranapanema and Pirajú.

  14. El ambulantaje en imágenes : una historia de representaciones de la venta callejera en la Ciudad de México (siglos XVIII-XX

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    Arnaud Exbalin

    2007-04-01

    Full Text Available Este trabajo es un análisis de las representaciones del ambulantaje (comercio ambulante, trabajo en la vía pública en la Ciudad de México, a través de la pintura de castas del siglo XVIII, la fotografía de finales del siglo XIX y principios del XX y la fotografía de prensa de finales del siglo XX. Este ensayo observa las transformaciones que esta actividad ha tenido en las diferentes épocas de desarrollo de la ciudad de México, a través del análisis de los productores de la imagen y del imaginario alrededor de este tipo de comercio, transmitido por medio de la pintura de castas o de la fotografía. Los tres artículos que conforman este ensayo, intentan indagar de forma general cómo se ha representado el ambulantaje en cada época, qué rasgos de esta actividad se modifican y cuáles se mantiene en el paso de un período a otro, de qué forma el análisis de las representaciones de una temporalidad nos ayuda a entender las imágenes propagadas en los siguientes periodos. Los autores abordaron principalmente tres dimensiones de la representación del ambulantaje para cada periodo: la social, la territorial y la económica. A pesar de las diferentes fuentes y perspectivas de análisis, logramos identificar aspectos que permanecen constantes en este largo periodo y que nos permiten entender la dinámica del fenómeno social de este tipo de comercio en la ciudad de México, dentro de un marco histórico y tradicional. Por otro lado, es concluyente que el ambulantaje es una actividad que se adapta a las cambiantes condiciones del espacio público, y que esta adaptabilidad hace posible su realización en los diferentes momentos del desarrollo urbano de la ciudad.

  15. A genética dos distúrbios do sono na infância e adolescência The genetics of sleep disorders in childhood and adolescence

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    Magda Lahorgue Nunes

    2008-08-01

    Full Text Available OBJETIVO: O objetivo deste artigo é revisar a literatura sobre a genética dos distúrbios do sono na infância e adolescência. FONTES DOS DADOS: As palavras-chave "sono" e "genética" foram usadas para pesquisar por artigos publicados nos últimos cinco anos no banco de dados MEDLINE. A seguir, seus resumos foram analisados. A pesquisa também incluiu artigos clássicos, com a primeira descrição dos genes. SÍNTESE DOS DADOS: A recorrência familiar de muitos distúrbios do sono é um achado freqüente, mas loci genéticos foram descobertos para poucos deles. Descrevemos aqui distúrbios do sono transmitidos por herança genética e também aqueles que apresentam altos índices de recorrência familiar, apesar de nenhum gene específico haver sido encontrado. CONCLUSÕES: Apesar da maioria dos distúrbios do sono ainda não terem uma base molecular identificada, técnicas modernas são cada vez mais utilizadas para determinar a contribuição dos genes ao sono e aos seus distúrbios associados. A importância clínica destas descobertas pode estar relacionada com a melhoria de métodos diagnósticos, mas também como alvo para o desenvolvimento de medicações específicas.OBJECTIVE: To review the literature regarding the genetics of sleep disorders in childhood and adolescence. SOURCES: Articles published in the past 5 years were searched on MEDLINE using the keywords sleep and genetics. Abstracts were then analyzed. Classical articles with the first description of genes were also included. SUMMARY OF THE FINDINGS: We often find familial recurrence in many sleep disorders. However, gene loci were discovered for only a few of them. We describe sleep disorders transmitted by genetic heritance and also those in that, although a gene was not found, familial recurrence is high. CONCLUSION: Although most of the sleep disorders do not have by now an identified molecular basis, modern techniques are being increasingly applied to determine the

  16. Diseño e implementación de una red de emuladores para recrear escenarios clínicos del sistema respiratorio de pacientes neonatales

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    Edward Julián Ramos Ballesteros

    2016-06-01

    Full Text Available Contexto/Objetivo: El objetivo es diseñar e implementar una red de emuladores para realizar a futuro un taller práctico con los estudiantes del área de la salud, colocando a prueba sus conocimientos y aprender de lo vivenciado antes de ejercer su vida profesional.Método: La red está compuesta por un Módulo de Control Central (MC, que permite al docente crear escenarios clínicos, y tres Emuladores para los estudiantes. Una vez creados los escenarios, son transmitidos hasta un Emulador que es escogido mediante una interfaz gráfica, con el fin de que el estudiante en práctica observe los valores y señales de los signos vitales tanto en la parte física (ME: Maniquí del Emulador como en la parte gráfica (IE: Interfaz del Emulador, y realice acciones dependiendo de los conocimientos adquiridos en sus estudios. Luego se genera un proceso de respuesta que le muestra al docente los resultados de las acciones. Finalmente el docente evalúa a los estudiantes comparando el escenario clínico con la respuesta y así también estimula el aprendizaje de los mismos.Resultados: Las características de implementación después de realizar un conjunto de pruebas son las siguientes: la distancia máxima de los componentes con respecto al MC, sin perder el óptimo funcionamiento de la red, es de 8 metros para el IE y 12 metros para el ME. Además con las técnicas implementadas se reduce en 13.28% el tiempo de ejecución cuando se programa el escenario clínico y en 21.78% el tiempo de ejecución del proceso de respuesta, estableciéndolos en 8,42 y 4,99 segundos respectivamente.Conclusiones: Teniendo en cuenta la distancia máxima y los tiempos de ejecución, se construye una red de emuladores con las características necesarias para implementar a futuro el taller práctico teniendo en cuenta las especificaciones técnicas, condiciones de tráfico, dimensiones de los salones, los usuarios en paralelo y la periodicidad con que se va a utilizar

  17. La institucionalización de la maternología en España durante la Segunda República y el franquismo The institutionalization of maternology in Spain during Second Republic and the Franco period

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    Carmen COLMENAR ORZAES

    2013-07-01

    Full Text Available Desde principios del siglo XX se desarrolló en España una campaña de divulgación socioeducativa, dirigida a las mujeres, mediante la cual se les trató de inculcar que la misión principal de sus vidas, por su propia naturaleza, era la maternidad. Dicha campaña integraba un discurso médico, amparado por un Estado patriarcal, que culpabilizaba a las mujeres de la mortalidad infantil, a causa de su ignorancia en las prácticas de crianza. El mensaje transmitido, a través de diversos medios de difusión, de la nueva ciencia de la maternología pretendía convertir a las mujeres en auténticas profesionales de la maternidad. Entre esos medios, ha centrado el interés de este artículo la Escuela Nacional de Puericultura, considerada en su evolución en el marco general de la época estudiada. Esta institución, en su triple vertiente docente, asistencial y de investigación, fue agente fundamental del desarrollo de la maternología en España.Since the early twentieth century it took place in Spain, a socio-educational spreading campaign, targeted to women, based on instilling that their primary mission in their lives, by their very nature, was maternity. This campaign incorporated a medical discourse, protected by a patriarchal Government, that blame women for infant mortality as a consequence of their ignorance in breeding practices. This message transmitted, through several spreading resources of the new science of maternology, pretended to convert women to real maternity professionals. Among these resources, the interest of this article is focused in the National Childcare School, considered on its evolution in the general framework of the studied period. This institution, in its triple dimension, educational, welfare and research, was fundamental agent of the development of the maternology (science of maternity in Spain.

  18. Técnicas moleculares para la detección e identificación de patógenos en alimentos: ventajas y limitaciones

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    Carolina Palomino-Camargo

    2014-09-01

    Full Text Available Las enfermedades transmitidas por alimentos, ocasionadas por microorganismos patógenos, constituyen un grave problema de salud pública a nivel mundial. Los métodos microbiológicos utilizados comúnmente en la detección de estos patógenos, de origen alimentario, son laboriosos y consume mucho tiempo. Esta situación, aunada a la demanda por resultados inmediatos y a los avances tecnológicos, ha conducido al desarrollo de una amplia gama de métodos rápidos en las últimas décadas. En base a esto, la presente revisión describe las ventajas y limitaciones de los principales métodos moleculares utilizados en la detección e identificación de microorganismos patógenos transmitidos por alimentos. Para ello, se consideró la actualidad de la información consultada, el análisis objetivo de la temática y su alcance. La literatura reciente reporta un número significativo de técnicas moleculares, alternativas, sensibles y selectivas para la detección, enumeración e identificación de microorganismos patógenos en alimentos, siendo la reacción en cadena de la polimerasa (PCR la plataforma más popular, mientras que la secuenciación de alto rendimiento se perfila como una técnica de gran aplicabilidad a futuro. Sin embargo, aun con todas las ventajas que ofrecen estas novedosas metodologías, no se deben pasar por alto sus limitaciones. Así, por ejemplo, los métodos moleculares no constituyen protocolos estandarizados, lo que dificulta su utilización en algunos casos. Por esta razón se debe trabajar arduamente para superar tales limitaciones y mejorar la aplicación de estas técnicas en matrices tan complejas como los sistemas alimenticios.

  19. Detección, identificación y localización geográfica de Begomovirus que afectan al tomate en Colombia

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    Juan Carlos Vaca-Vaca

    2011-12-01

    Full Text Available Detection, identification and geographical localization of tomato-infecting Begomovirus in Colombia Título corto: Begomovirus que afectan tomate en Colombia ResumenEn la actualidad, los Begomovirus (Familia Geminiviridae se han convertido en la mayor amenaza para los cultivos de interés agrícola ubicados en las zonas tropicales y templadas del planeta. Estos virus son transmitidos por la mosca blanca Bemisia tabaci, la cual en los últimos años en Colombia ha tenido un aumento significativo en sus poblaciones y se ha asociado con la aparición de síntomas virales en cultivos de tomate. Muestras de tomate con síntomas virales típicos fueron recolectadas en las cinco principales zonas productoras de esta solanácea en el país. Los Begomovirus fueron detectados por medio de la técnica de hibridación de ácidos nucleicos tipo Dot blot así como por medio de la reacción en cadena de la polimerasa (PCR en todas las muestras colectadas. Con la excepción de un reporte previo en el Valle del Cauca, este es el primer reporte de Begomovirus afectando cultivos de tomate en los departamentos de Antioquia, Santander, Boyacá y Cundinamarca. Asimismo, es la primera vez que se informa sobre Begomovirus que afectan cultivos de tomate localizados por encima de 1500 msnm en Colombia. Palabras clave: Begomovirus, Bemisia tabaci, tomate, Dot blot, PCR. Abstract The begomoviruses (Family Geminiviridae have recently emerged as  Key words: Begomovirus, Bemisia tabaci, tomato, Dot blot, PCR. samples with typical Begomovirus symptoms were collected in five different departments, comprising the mayor tomato growing areas of the country. Begomovirus were detected by Polymerase Chain Reaction (PCR or Dot Blot Hybridization in all tomato samples collected in whole tomato growing areas of the country. With exception for Valle del Cauca, this is the first report of tomato-infecting Begomovirus in Antioquia, Santander, Boyacá and Cundinamarca departments

  20. Memoria histórica vivida y transmitida en torno a los terremotos de 1939-1960 en los habitantes del Gran Concepción – Chile

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    Víctor Andrés Concha Ramírez

    2011-04-01

    Full Text Available El artículo forma parte de una investigación mayor dirigida por Stefano Cavalli y Christian Lalive d`Epinay en el marco de la red internacional CEVI (Cambios y eventos en el transcurso de la vida. Una sección del estudio refiere a la importancia y selectividad otorgada a acontecimientos y procesos socio-históricos vividos y transmitidos. Se han encuestado 623 individuos de cinco grupos de edad pertenecientes a comunas del Gran Concepción (Chile. Desde los datos han surgido antecedentes relevantes en relación a los terremotos de 1939 en Chillán y 1960 en Valdivia y Concepción que serán analizados en este artículo. El tema resulta particularmente sensible en el tiempo presente, dadas las consecuencias vividas por una reciente catástrofe ocurrida el 27 de Febrero de 2010 en la región del Bio Bio. Palabras claves: Terremotos en Chile, Memoria Histórica, Memoria Colectiva___________________________Abstract:The article is part of a major research o survey lead by Stefano Cavalli y Christián Lalive d`Epinay in the framework of the international network CEVI (Changes and events life course. A section of the research refers to the importance and selectivity granted to events and processes socio-historical processes lived and transmitted. Were surveyed 623 individuals of five age groups belonging to the communes of the big Concepcion (Chile. From the data has been emerged relevant background in relation with earthquakes of 1939 in Chillán and 1960 Valdivia and Concepción that which will be to be analyzed in this article. The topic is particularly sensitive in the present tense, because the consequences experienced by a recent catastrophe occurred on February 27, 2010 in the region of Bio Bio.Keywords: Earthquake in Chile, historical memory, collective memory.

  1. Ser respetuoso es ser persona. El niño y la pedagogía moral de los nahuas del centro de México

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    Lorente Fernández, David

    2012-12-01

    diversas actividades cotidianas. A pesar de estar sometidos al respeto «mestizo» transmitido en el interior de las escuelas, entre los niños la moralidad nahua se impone como algo «dado» y constitutivo de la manera de ser serrana.

  2. Television y bebidas alcohólicas y analcohólicas Television and drinking-alcoholic and nonalcoholic

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    Pedro Naveillan F.

    1987-02-01

    Full Text Available Dadas las implicancias educativas de la televisión, se decidió estudiar la frecuencia y características de los eventos con bebidas alcohólicas y analcohólicas en la televisión chilena utilizando el método ideado por Garlington. El registro fue hecho por períodos de media hora, distribuidos aleatoriamente, para cada observador, voluntaria perteneciente a una comuna que se caracteriza por su bajo nivel sociocultural; ellas monitorearon durante una semana, de Lunes a Viernes todos los programas transmitidos desde las 20:00 a las 24:00 horas. Se observó que, en promedio, por cada canal se transmite un evento de bebida alcohólica cada 24 minutos 19 segundos y uno de bebida no alcohólica cada 37 minutos y medio; las imágenes de bebida alcohólicas se incrementan a partir de las 21:30 horas. Del total de eventos, 60,7% corresponden a bebidas alcohólicas y de éstos el 61,1% tiene carácter publicitario. Los eventos relacionados con bebidas alcohólicas ocurren principalmente en la casa, por amistad y en forma de escenas, los con no alcohólicas en lugares desportivos, por amistad y en escena de ingestión.In view of the educational impact of television, the frequency and characteristics of T.V. events associated both with alcoholic and non-alcoholic beverages were studied, according to Garlington's technique. The events in all the programs from 8 p.m. to 12 midnight, Monday through Friday, were registered by volunteers from an area of low socioeconomic status, in probabilisticably selected half an hour periods during a normal week. On the average the channels transmit an alcohol-related event every 24'19", and one non-alcoholic beverage associated event every 37'30". Alcohol drinking appears more often ofter 9:30 p.m. Of the total number of events, 60.7% are related to alcohol, 61.1% of these being advertisements. They take place mainly in the home or in a friendly atmosphere, under the guise of plays; non-alcoholic beverages were shown at

  3. Implantação da linha de cuidado do infarto agudo do miocárdio no município de Belo Horizonte

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    Milena Soriano Marcolino

    2013-04-01

    Full Text Available FUNDAMENTO: A criação de sistemas ou linhas de cuidado ao infarto agudo do miocárdio (IAM tem o objetivo de otimizar o atendimento ao paciente, desde o diagnóstico precoce até o tratamento adequado e em tempo hábil. OBJETIVO: Avaliar a implantação da linha de cuidado do IAM, em Belo Horizonte, Minas Gerais, e seu impacto na mortalidade hospitalar por IAM. MÉTODOS: A linha de cuidado do IAM foi implantada em Belo Horizonte entre 2010 e 2011 com intuito de ampliar o acesso dos pacientes do sistema público de saúde ao tratamento preconizado pelas diretrizes vigentes. As equipes das unidades de pronto atendimento foram treinadas e foi implantado sistema de tele-eletrocardiografia nessas unidades. Os desfechos primários deste estudo observacional retrospectivo foram o número de internações e a mortalidade hospitalar por IAM, de 2009 a 2011. RESULTADOS: No período avaliado, 294 profissionais foram treinados e 563 ECGs foram transmitidos das unidades de pronto atendimento para as unidades coronarianas. Houve redução importante da taxa de mortalidade hospitalar (12,3% em 2009 versus 7,1% em 2011, p < 0,001, enquanto o número de internações por IAM permaneceu estável. Ocorreu aumento do custo médio de internação (média R$ 2.480,00 versus R$ 3.501,00, p < 0,001, aumento da proporção de internações contemplando diárias de terapia intensiva (32,4% em 2009 versus 66,1% em 2011, p < 0,001 e de pacientes internados em hospitais de alta complexidade (47,0% versus 69,6%, p < 0,001. CONCLUSÃO: A implantação da linha de cuidado do IAM permitiu maior acesso da população ao tratamento adequado e, consequentemente, redução na mortalidade hospitalar por IAM.

  4. Epidemiologia molecular de surto de raiva bovina na região central do Rio Grande do Sul, 2012

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    Fábio Adriano Kanitz

    2014-05-01

    Full Text Available A raiva é uma doença infecciosa do sistema nervoso central de mamíferos causada pelo vírus da raiva (RabV, geralmente, transmitido pela mordedura de animais infectados. No Brasil, os morcegos hematófagos Desmodus rotundus são as principais fontes de infecção do RabV para bovinos e equinos. Este artigo descreve uma investigação epidemiológica e molecular de surtos de raiva ocorridos na região central do Rio Grande do Sul, entre maio e agosto de 2012. Nesse período, 45 casos suspeitos de raiva foram relatados em 22 pequenos rebanhos, localizados dentro de um raio de 4,7km, no município de Pinhal Grande. Desses, 32 amostras foram submetidas para diagnóstico da raiva, sendo que o RabV e/ou antígenos virais foram identificados em 27 amostras. Em um segundo momento, 11 amostras foram submetidas à transcrição reversa/reação em cadeia da polimerase (RT-PCR para o gene da nucleoproteína (N do RabV, seguido de sequenciamento nucleotídico e análise filogenética. Sete das 11 amostras apresentaram sequências nucleotídicas idênticas e uma apresentou mutação sinônima, não-codificante, indicando uma provável origem comum dos vírus. Por outro lado, três amostras apresentaram mutações que resultaram em alterações de aminoácidos, sugerindo uma origem diferente do vírus. Esses resultados sugerem que RabV de diferentes origens/linhagens co-circulam na região e foram envolvidos nos surtos descritos. Investigações sobre a circulação de ambos os genótipos em morcegos na região estão em andamento.

  5. Discursos do poder, política educacional e os livros didáticos de leitura no Rio Grande do Sul (1930-1945 - Discourses of power, international politics, and reading educational books in Rio Grande do Sul (1930-1945

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    Berenice Corsetti, Elisabete Magda Klaus

    2011-03-01

    Full Text Available Resumo Este trabalho trata da relação percebida entre os discursos pronunciados pelos dirigentes maiores da educação brasileira, no período de 1930 a 1945, os quais explicitaram os pressupostos da política educacional então vigente, e os manuais didáticos de leitura utilizados nas escolas primárias do Rio Grande do Sul. Buscou-se perceber o papel por eles desenvolvidos na construção do ideário legitimador do modelo sócio-econômico e político, à época, bem como do imaginário coletivo que respaldou a consolidação desse modelo. Através de categorias teóricas que sistematizaram as informações, percebemos o universo de valores transmitidos às crianças das escolas primárias, preparando-as para a sua inserção ordeira e disciplinada na sociedade de então. Palavras-chave: livros de leitura; Rio Grande do Sul; política educacional.   DISCOURSES OF POWER, INTERNATIONAL POLITICS, AND READING EDUCATIONAL BOOKS IN RIO GRANDE DO SUL (1930-1945 Abstract This paper is about the connection that has been noticed relating the discourses pronounced by the most important leaders of the Brazilian education from 1930 to 1945, that clearly stated the presuppositions of the educational politics which were current on that time, and the reading educational manuals that were used at the primary schools in Rio Grande do Sul. We have tried to notice the role played by them on building up the legitimizing ideas of the political and socioeconomic model of that time and also the collective ideas which supported the consolidation of this model. Having as our base the theoretical categories that systematized the information, we noticed the universe of values that was transmitted to the children who studied in the primary schools, preparing them to become an orderly and disciplined part of the society of that time. Keywords: reading books; Rio Grande do Sul; educational politics.   DISCURSOS DE PODER, POLÍTICA EDUCATIVA Y LOS LIBROS DIDÁCTICOS DE

  6. A amamentação nos manuais escolares de estudo do meio do 1º ciclo do ensino básico La lactancia en los libros de texto escolares del Estudio del Medio del 1º ciclo de la educación básica Breastfeeding in Environmental Studies textbooks used in the first cycle of basic education

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    Dulce Maria Pereira Garcia Galvão

    2011-07-01

    Full Text Available Para conhecer como a amamentação é abordada às crianças do 1º Ciclo, e o que aprendem na escola sobre este tema, analisaram-se, do 1º ao 4º ano de escolaridade, 19 manuais escolares de Estudo do Meio, adoptados em 73 escolas públicas de seis agrupamentos de escolas da área educativa de Coimbra. Na análise dos conceitos transmitidos definiram-se, a priori, alguns considerados imprescindíveis para ensinar e explicar à criança noções básicas de amamentação. Apenas dois manuais do 3º ano incluíam, cada um, os conteúdos “Somos mamíferos. Alimentamo-nos das mamas das nossas mães após o nascimento” e “O natural é tomar leite materno logo depois do nascimento”. A análise dos manuais permitiu observar que existem diversas oportunidades de ensinar, esclarecer e ajudar a criança a aprender uma cultura da amamentação, pois este tema pode estar incluído em diversos capítulos das diferentes rubricas programáticas. No entanto, estas oportunidades são desperdiçadas e neste material didáctico várias imagens utilizadas promovem a alimentação artificial.Para conocer cómo se les presenta la lactancia a los niños del y lo que aprenden en la escuela sobre este tema, se analizaron, del 1º al 4º año de escolaridad, 19 libros de texto escolares del Estudio del Medio, adoptados en 73 escuelas públicas de seis grupos de escuelas del área educativa de Coimbra. En el análisis de los conceptos transmitidos se definieron, a priori, algunos considerados imprescindibles para enseñar y explicarle al niño nociones básicas de lactancia. Solo dos libros de texto del 3º año incluían, en cada caso, los contenidos “Somos mamíferos. Nos alimentamos del pecho de nuestras madres después de nacer” y “Lo natural es tomar leche materna después de haber nacido”. El análisis de los libros de texto permitió observar que existen diversas oportunidades de enseñar, aclarar e ayudar al niño a aprender una cultura de la

  7. Spanish Editorial

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    Littera Aperta

    2013-09-01

    Full Text Available Emprender la singladura de una nueva revista sobre estudios literarios y humanísticos es siempre un proyecto incierto, pero más, si cabe, en un contexto, como el actual, de crisis económica, desánimo colectivo y descrédito creciente de las Humanidades en la sociedad. Son malos tiempos para la lírica (“schlechte Zeit für Lyrik”, según escribió premonitoriamente Bertolt Brecht allá por los años ‘20 del pasado siglo y luego cantó con bastante más eco mediático el grupo Golpes Bajos por los ‘80. Sin embargo, estamos convencidos de que el desaliento, como el fuego, se expande si se le da fuelle: el optimismo, por su parte, tiene una virtud performativa. En cualquier caso, no se olvide que ya el adagio latino, transmitido por Ausonio, nos enseña que dimidium est facti coepisse: comenzar es la mitad de la acción. Queremos que Littera Aperta haga de su título seña de identidad, acogiendo un amplio espectro de metodologías analíticas, desde aproximaciones antiguas, marcadamente filológicas, hasta estrategias modernas y también postmodernas. Tradicionalmente, la filología ha pretendido establecer los textos y reconstruir su significado en su contexto histórico original, mientras que la crítica posmoderna estima que el significado de los textos no es otra cosa que un constructo cultural. Sin embargo, sostenemos que en la travesía crítica es posible surcar una ruta equidistante entre esas Escila y Caribdis: el análisis de los textos nos ayuda a construir teorías y, complementariamente, la duda metódica de la deconstrucción nos urge a probar la validez de las teorías mediante una lectura crítica de los textos. Admitamos, con Pierre Grimal, que los sistemas envejecen y quedan obsoletos, a veces con extrema rapidez y que solo los datos permanecen inmutables, como posesión para siempre, según Tucídides consideró a la Historia. Pero no es menos cierto que los sistemas, teorías y modelos nos ayudan a poner Orden en el

  8. A relação entre tecnologias sociais e economia solidária: um estudo de caso em uma cooperativa de catadores de resíduos

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    Rafaela Francisconi Gutierrez

    2013-04-01

    Full Text Available As cooperativas de catadores de resíduos sólidos são empreendimentos, que por serem organizadas e realizadas solidariamente por seus trabalhadores e trabalhadoras, de forma coletiva e autogestionária, fazem parte do que se denomina, no Brasil, de Economia Solidária. Este trabalho tem por objetivo identificar aspectos da relação entre as tecnologias existentes nestas cooperativas e os valores da economia solidária. Para isto foi estudado o estágio atual de desenvolvimento de uma cooperativa de catadores, situada no município de São Carlos/SP, considerando os seguintes aspectos: processo de coleta, triagem e comercialização; organização do trabalho; tecnologias utilizadas e desenvolvidas e os apoios e parcerias existentes. Para a coleta de dados foi utilizada a técnica observacional e de entrevistas. Como resultado foi observado que os cooperados se apropriaram das tecnologias existentes. Quanto aos aspectos da economia solidária, eles possuem a propriedade coletiva dos meios de produção e fazem a gestão da cooperativa; não há divisão técnica do trabalho: todos os sócios aprendem e são capazes de exercer as diferentes atividades dentro da cooperativa; e os conhecimentos necessários para o desenvolvimento das atividades são transmitidos entre os cooperados.Palavras-chave | Adequação sócio-técnica; catadores de resíduos; cooperativas; economia solidária; tecnologias sociais.Código JEL | J21; 032; P13. THE RELATIONSHIP BETWEEN SOCIAL TECHNOLOGIES AND SOLIDARITY ECONOMY: A CASE STUDY IN COOPERATIVE OF WASTE PICKERSAbstractThe cooperatives of recyclable solid waste projects, which are organized and conducted jointly by their workers, in a self-managed and collectively way, are part of what is called in Brazil, Solidarity Economy. This study aims at identifying aspects of the relationship between the existing technologies in these cooperatives and the values of solidarity economy. In order to do so we have studied the

  9. Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo Frequency of Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus in symptomatic and assymptomatic lettuce plants and sap and soil transmission

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    Márcio Martinello Sanches

    2007-12-01

    Full Text Available A presença de sintomas de 'big vein' ou engrossamento das nervuras em alface e a associação do Lettuce big-vein associated virus (LBVaV e Mirafiori lettuce big-vein virus (MLBVV foram verificadas por RT-PCR utilizando-se oligonucleotídeos específicos para ambos os vírus. Foram coletadas 366 plantas sintomáticas nas regiões de Bauru, Campinas e Mogi das Cruzes no estado de São Paulo nos meses de junho e setembro de 2004 e abril e julho de 2005, e 18 plantas assintomáticas na região de Mogi das Cruzes no mês de dezembro de 2004. Infecção mista do LBVaV e MLBVV foi detectada em 76,2% das plantas sintomáticas, em 11,5% somente o MLBVV foi detectado e em 6,6% somente o LBVaV. Nas plantas assintomáticas coletadas em dezembro na região de Mogi das Cruzes, em áreas de alta incidência da doença durante o inverno, foi detectada a presença de MLBVV em 9 amostras e LBVaV em 7 amostras. Quatro dessas amostras apresentaram infecção mista, indicando que o desenvolvimento de sintomas depende de fatores abióticos, como temperatura. A inoculação via extrato vegetal a partir de plantas de alface com infecção mista pelo MLBVV e LBVaV foi realizada a 16°C/ 10°C (dia/noite e fotoperíodo de 11 horas, verificando-se que o MLBVV foi transmitido para Nicotiana tabacum TNN, N. rustica, N. occidentalis, Chenopodium quinoa e para as cultivares de alface Trocadero e White Boston, enquanto o LBVaV foi transmitido apenas para a alface White Boston. Entretanto, nenhuma destas plantas apresentou sintomas da doença, com exceção de C. quinoa que apresentou sintomas de lesões locais. Plantio em solo proveniente de áreas com histórico da doença de Mogi das Cruzes permitiu a transmissão do LBVaV para alface cv. White Boston e do MLBVV para alface cv. Trocadero e White Boston, embora apenas a cv. White Boston tenha apresentado sintomas da doença. Em conjunto, estes resultados demonstram a dificuldade de transmissão de ambos os vírus, mesmo em

  10. Ocorrência do vírus do mosqueado do morangueiro no estado de São Paulo Occurrence of the strawberry mottle virus in São Paulo

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    Ana Maria B. Carvalho

    1961-01-01

    Full Text Available Verificou-se a ocorrência de estirpes do vírus do grupo denominado mosqueado («strawberry mottle» em plantações de morangueiro no Estado de São Paulo. Variedades antigas, como a Dr. Morère. acham-se totalmente infetatas. sendo portadoras sem sintomas. Alguns clones novos plantados apenas por poucos anos em campo, já se acham parcialmente infetados, indicando que há transmissão da moléstia sob condições naturais. Sintomas de palidez das nervuras, mosqueado, paralisação no crescimento e encrespamento são apresentados por plantas de Fragaria vesca infetadas pelos vírus dêsse grupo. Numerosas espécies de plantas-teste habituais foram inoculadas com diferentes isolados do vírus, por meio do vetor, mas os resultados foram geralmente negativos. Afídios virulíferos, colonizados sôbre plantas novas de Cassia accidentalis, Chenopodiam quinoa, Leonotis nepaetifolia e Leonurus sibiricus. induziram o aparecimento de sintomas. Não se conseguiu retransmitir o vírus dessas espécies para F. vesca, existindo, portanto, dúvidas sôbre a verdadeira identidade do vírus que infetava tais plantas. O vírus do mosqueado não foi aparentemente transmitido pela semente. Também não se mostrou transmissível mecânicamente para Frogaria vesca. O virus obtido por inoculação com o vetor em Chenopodium quinoa e que se supõe ser o do mosqueado, pôde se transmitido mecânicamente de C. quinoa para C. quinoa. mas não para F. vesca. O pulgão Pentatrichopus fragaefolii mostrou-se eficiente vetor do mosqueado, conseguindo-se obter em média mais de 50% de infecção em infestações com 1 afidio por planta. Aphis gossypii também transmitiu o vírus do mosqueado, mas com muito menor eficiência. Não se conseguiu transmitir o mosqueado com uma espécie de Cuscuta que ocorre comumeute em Campinas. Em testes de transmissão por enxertia de fôlhas, os resultados foram muito fracos devido ao mau pegamento. O pulgão Pentatrichopus fragaefolii

  11. Donkey orchid symptomless virus: a viral 'platypus' from Australian terrestrial orchids.

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    Stephen J Wylie

    Full Text Available Complete and partial genome sequences of two isolates of an unusual new plant virus, designated Donkey orchid symptomless virus (DOSV were identified using a high-throughput sequencing approach. The virus was identified from asymptomatic plants of Australian terrestrial orchid Diuris longifolia (Common donkey orchid growing in a remnant forest patch near Perth, western Australia. DOSV was identified from two D. longifolia plants of 264 tested, and from at least one plant of 129 Caladenia latifolia (pink fairy orchid plants tested. Phylogenetic analysis of the genome revealed open reading frames (ORF encoding seven putative proteins of apparently disparate origins. A 69-kDa protein (ORF1 that overlapped the replicase shared low identity with MPs of plant tymoviruses (Tymoviridae. A 157-kDa replicase (ORF2 and 22-kDa coat protein (ORF4 shared 32% and 40% amino acid identity, respectively, with homologous proteins encoded by members of the plant virus family Alphaflexiviridae. A 44-kDa protein (ORF3 shared low identity with myosin and an autophagy protein from Squirrelpox virus. A 27-kDa protein (ORF5 shared no identity with described proteins. A 14-kDa protein (ORF6 shared limited sequence identity (26% over a limited region of the envelope glycoprotein precursor of mammal-infecting Crimea-Congo hemorrhagic fever virus (Bunyaviridae. The putative 25-kDa movement protein (MP (ORF7 shared limited (27% identity with 3A-like MPs of members of the plant-infecting Tombusviridae and Virgaviridae. Transmissibility was shown when DOSV systemically infected Nicotiana benthamiana plants. Structure and organization of the domains within the putative replicase of DOSV suggests a common evolutionary origin with 'potexvirus-like' replicases of viruses within the Alphaflexiviridae and Tymoviridae, and the CP appears to be ancestral to CPs of allexiviruses (Alphaflexiviridae. The MP shares an evolutionary history with MPs of dianthoviruses, but the other putative

  12. Donkey orchid symptomless virus: a viral 'platypus' from Australian terrestrial orchids.

    Science.gov (United States)

    Wylie, Stephen J; Li, Hua; Jones, Michael G K

    2013-01-01

    Complete and partial genome sequences of two isolates of an unusual new plant virus, designated Donkey orchid symptomless virus (DOSV) were identified using a high-throughput sequencing approach. The virus was identified from asymptomatic plants of Australian terrestrial orchid Diuris longifolia (Common donkey orchid) growing in a remnant forest patch near Perth, western Australia. DOSV was identified from two D. longifolia plants of 264 tested, and from at least one plant of 129 Caladenia latifolia (pink fairy orchid) plants tested. Phylogenetic analysis of the genome revealed open reading frames (ORF) encoding seven putative proteins of apparently disparate origins. A 69-kDa protein (ORF1) that overlapped the replicase shared low identity with MPs of plant tymoviruses (Tymoviridae). A 157-kDa replicase (ORF2) and 22-kDa coat protein (ORF4) shared 32% and 40% amino acid identity, respectively, with homologous proteins encoded by members of the plant virus family Alphaflexiviridae. A 44-kDa protein (ORF3) shared low identity with myosin and an autophagy protein from Squirrelpox virus. A 27-kDa protein (ORF5) shared no identity with described proteins. A 14-kDa protein (ORF6) shared limited sequence identity (26%) over a limited region of the envelope glycoprotein precursor of mammal-infecting Crimea-Congo hemorrhagic fever virus (Bunyaviridae). The putative 25-kDa movement protein (MP) (ORF7) shared limited (27%) identity with 3A-like MPs of members of the plant-infecting Tombusviridae and Virgaviridae. Transmissibility was shown when DOSV systemically infected Nicotiana benthamiana plants. Structure and organization of the domains within the putative replicase of DOSV suggests a common evolutionary origin with 'potexvirus-like' replicases of viruses within the Alphaflexiviridae and Tymoviridae, and the CP appears to be ancestral to CPs of allexiviruses (Alphaflexiviridae). The MP shares an evolutionary history with MPs of dianthoviruses, but the other putative

  13. Tahyna virus genetics, infectivity, and immunogenicity in mice and monkeys

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    Whitehead Stephen S

    2011-03-01

    Full Text Available Abstract Background Tahyna virus (TAHV is a human pathogen of the California encephalitis virus (CEV serogroup (Bunyaviridae endemic to Europe, Asia, and Africa. TAHV maintains an enzootic life cycle with several species of mosquito vectors and hares, rabbits, hedgehogs, and rodents serving as small mammal amplifying hosts. Human TAHV infection occurs in summer and early fall with symptoms of fever, headache, malaise, conjunctivitis, pharyngitis, and nausea. TAHV disease can progress to CNS involvement, although unlike related La Crosse virus (LACV, fatalities have not been reported. Human infections are frequent with neutralizing antibodies present in 60-80% of the elderly population in endemic areas. Results In order to determine the genomic sequence of wild-type TAHV, we chose three TAHV isolates collected over a 26-year period from mosquitoes. Here we present the first complete sequence of the TAHV S, M, and L segments. The three TAHV isolates maintained a highly conserved genome with both nucleotide and amino acid sequence identity greater than 99%. In order to determine the extent of genetic relatedness to other members of the CEV serogroup, we compared protein sequences of TAHV with LACV, Snowshoe Hare virus (SSHV, Jamestown Canyon virus (JCV, and Inkoo virus (INKV. By amino acid comparison, TAHV was most similar to SSHV followed by LACV, JCV, and INKV. The sequence of the GN protein is most conserved followed by L, N, GC, NSS, and NSM. In a weanling Swiss Webster mouse model, all three TAHV isolates were uniformly neurovirulent, but only one virus was neuroinvasive. In rhesus monkeys, the virus was highly immunogenic even in the absence of viremia. Cross neutralization studies utilizing monkey immune serum demonstrated that TAHV is antigenically distinct from North American viruses LACV and JCV. Conclusions Here we report the first complete sequence of TAHV and present genetic analysis of new-world viruses, LACV, SSHV, and JCV with old

  14. Pseudo-plaque reduction neutralization test (PPRNT for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus

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    Canakoglu Nurettin

    2013-01-01

    Full Text Available Abstract Background Crimean-Congo hemorrhagic fever virus (CCHFV is a tick-borne virus of the genus Nairovirus family Bunyaviridae, which are enveloped viruses containing tripartite, negative polarity, single-stranded RNA. CCHF is characterized by high case mortality, occurring in Asia, Africa, the Middle East and Europe. Currently, there are no specific treatments or licensed vaccines available for CCHFV. Recently, two research groups have found adult mice with defective interferon responses allowed to lethal CCHFV infection. These mouse models could provide invaluable information for further studies. Efforts to develop a vaccine against CCHFV are being made. To determine the efficacy of vaccine candidates it is important to conduct serological studies that can accurately measure levels of protective antibodies. In the present study, a pseudo-plaque reduction neutralization test (PPRNT based on enzyme-catalyzed color development of infected cells probed with anti-CCHFV antibodies was used to measure neutralization antibody of CCHFV. Methods Sixty-nine human serum samples (20 acute and 49 convalescent were tested. The presence of CCHFV antibodies was determined and confirmed by a commercial ELISA kit. CCHFV RNA was determined by RT-PCR. All the samples were analyzed by PPRNT and fluorescent focus reduction neutralization test (FFRNT to measure of CCHFV-neutralizing antibodies. Results Pseudo-plaque reduction neutralization test showed a high sensitivity (98%, specificity (100% and agreement (96,6% in qualitative comparison with those of the FFRNT. There was a high correlation between the titers obtained in PPRNT and FFRNT (R2 = 0.92. The inter- and intra-assay variation of PPRNT revealed good reproducibility and positive cut-off of PPRNT was defined as 1:4 by the geometric mean titers for the individual samples distributed. Conclusion The pseudo-plaque reduction neutralization test described in this study is a fast, reproducible and sensitive

  15. [Hantavirus infection: two case reports from a province in the Eastern Black Sea Region, Turkey].

    Science.gov (United States)

    Kaya, Selçuk; Yılmaz, Gürdal; Erensoy, Sükrü; Yağçı Çağlayık, Dilek; Uyar, Yavuz; Köksal, Iftihar

    2010-07-01

    Hantaviruses which are the members of Bunyaviridae, differ from other members of this family since they are transmitted to humans by rodents. More than 200.000 cases of hantavirus infections are reported annually worldwide. Hantaviruses can lead to two different types of infection in humans, namely, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). HFRS is the most common type of hantavirus infection in Europe and Asia and the most common virus types are Dobrava, Puumala, Hantaan and Seoul. A total of 25 hantavirus suspected cases have been reported from the Western Black Sea region of Turkey and 12 of these were confirmed serologically as "Puumala" subtype. Serological tests such as indirect immunofluorescence assay (IFA), are used for diagnosis and typing of the hantaviruses, however, since cross-reactions are common between the subtypes, the results of these tests should be confirmed by other methods. In this report two cases with hantavirus infection defined serologically were presented. Two male patients, 55 and 50 years old, respectively, living in Giresun province of Eastern Black Sea region, Turkey, were admitted to the State Hospital with the complaints of fever, sweating and diarrhoea without blood or mucus. Since thrombocytopenia and renal failure were detected in these two cases, they were transferred to the University Hospital. Presence of fever, thrombocytopenia and renal failure, with no laboratory findings of a bacterial infection and no growth of microoorganisms in the clinical specimens, admittance of the patients during summer and history of being present in the fields, necessitated to rule out leptospirosis, Crimean Kongo hemorrhagic fever and hantavirus infection which were all endemic in our area. Further investigation of the serum samples at the National Reference Virology Laboratory by IFA (Hantavirus Mosaic-1, Euroimmun, Germany) revealed hantavirus IgM and IgG antibodies ≥ 1:100 titer and the results

  16. [Prognostic factors in hantavirus infections].

    Science.gov (United States)

    Kaya, Selçuk

    2014-01-01

    The hantaviruses classified in Hantavirus genus of Bunyaviridae family, may cause two different types of clinical conditions, namely hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Mortality may reach up to 40% in these infections. Hantavirus subtypes (Sin Nombre, Hantaan, Seoul, Puumala, Dobrava, etc) with different virulences represent one of the most significant factors affecting the mortality. Additionally, many other factors including age, gender, humoral immune response, genetic factors, patient's clinical and laboratory findings, transfusion, mechanical ventilation requirement, antiviral treatment and immunotherapy administered to the patient are prognostically important. Increasing age had an unfavorable effect on mortality. While the disease is commonly observed in the male gender, mortality rate is higher in the female gender. The higher the emergent neutralizing antibody response, the virus spread, the number of the infected cells and the cytotoxic T lymphocyte-mediated injury will be lower. The requirement for dialysis is reported to be higher with a poorer prognosis in individuals with HLA-B8, -DR3, -DQ2 alleles, and those with HLA-B27 allele usually experience a milder clinical course. Clinically, the risk of mortality increases in patients with multiple, central nervous system hemorrhage, sepsis, disseminated intravascular coagulation (DIC) and secondary infection. The presence of adult respiratory distress syndrome (ARDS), the requirement for mechanical ventilation, the presence of dyspnea and hemoconcentration in HPS are reported to be the most important prognostic factors associated with death. The correlation of severity and the transfusion requirement with mortality was demonstrated. High serum levels of white blood cells, blood urea nitrogen (BUN), creatinine phophokinase (CPK), C-reactive protein (CRP), prothrombin time (PT), activated partial thromboplastin time (aPTT), D-dimer and INR (International

  17. The two envelope membrane glycoproteins of Tomato spotted wilt virus show differences in lectin-binding properties and sensitivities to glycosidases

    International Nuclear Information System (INIS)

    Tomato spotted wilt virus (TSWV, Genus: Tospovirus, Family: Bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide. The amino acid sequence of the two envelope membrane glycoproteins, designated as GN (N-terminal) and GC (C-terminal), of TSWV contain several tripeptide sequences, Asn-Xaa-Ser/Thr, suggesting that the proteins are N-glycosylated. In this study, the lectin-binding properties of the viral glycoproteins and their sensitivities to glycosidases were examined to obtain information on the nature of potential oligosaccharide moieties present on GN and GC. The viral proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and probed by affinoblotting using a battery of biotinylated lectins with specificity to different oligosaccharide structures. GC showed strong binding with five mannose-binding lectins, four N-acetyllactosamine-binding lectins and one fucose-binding lectin. GN was resolved into two molecular masses and only the slow migrating form showed binding, albeit to a lesser extent than GC, with three of the five mannose-binding lectins. The N-acetyllactosamine- and fucose-specific lectins did not bind to either molecular mass form of GN. None of the galactose-, N-acetylgalactosamine-, or sialic acid-binding lectins tested showed binding specificity to GC or GN. Treatment of the denatured virions with endoglycosidase H and peptide:N-glycosidase F (PNGase F) resulted in a significant decrease in the binding of GC to high mannose- and N-acetyllactosamine-specific lectins. However, no such differences in lectin binding were apparent with GN. These results indicate the presence of N-linked oligosaccharides of high mannose- and complex-type on GC and possibly high mannose-type on GN. Differences in the extent of binding of the two envelope glycoproteins to different lectins suggest that GC is likely to be more heavily N-glycosylated than

  18. Differential expression of tomato spotted wilt virus-derived viral small RNAs in infected commercial and experimental host plants.

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    Neena Mitter

    Full Text Available BACKGROUND: Viral small RNAs (vsiRNAs in the infected host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different hosts infected by the same virus influencing viral pathogenicity and host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV, a segmented negative stranded RNA virus in the family Bunyaviridae, with two of its gene segments showing ambisense gene arrangement. The virus causes significant economic losses to numerous field and horticultural crops worldwide. PRINCIPAL FINDINGS: Tomato spotted wilt virus (TSWV-specific vsiRNAs were characterized by deep sequencing in virus-infected experimental host Nicotiana benthamiana and a commercial, susceptible host tomato. The total small (s RNA reads in TSWV-infected tomato sample showed relatively equal distribution of 21, 22 and 24 nt, whereas N. benthamiana sample was dominated by 24 nt total sRNAs. The number of vsiRNA reads detected in tomato was many a magnitude (~350:1 higher than those found in N. benthamiana, however the profile of vsiRNAs in terms of relative abundance 21, 22 and 24 nt class size was similar in both the hosts. Maximum vsiRNA reads were obtained for the M RNA segment of TSWV while the largest L RNA segment had the least number of vsiRNAs in both tomato and N. benthamiana. Only the silencing suppressor, NSs, of TSWV recorded higher antisense vsiRNA with respect to the coding frame among all the genes of TSWV. SIGNIFICANCE: Details of the origin, distribution and abundance of TSWV vsiRNAs could be useful in designing efficient targets for exploiting RNA interference for virus resistance. It also has major implications toward our understanding of the differential processing of vsi

  19. Sorologia e sobrevivência do vírus do amarelo letal do mamoeiro Serology and surviving characteristics of Papaya lethal yellowing virus

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    Ana Lúcia Lopes do Nascimento

    2010-09-01

    Full Text Available O vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV é responsável por uma das principais doenças do mamoeiro (Carica papaya no Nordeste brasileiro. O PLYV pode ser transmitido através do solo, água, instrumento de corte e mãos contaminadas. A presente pesquisa teve como objetivo estudar as características biológicas, sorológicas e físicas de um isolado do vírus e avaliar sua sobrevivência em tecido seco infetado. O PLYV foi detectado por "Enzyme linked immunosorbent assay" (ELISA indireto e isolado em mudas de mamoeiro, através da inoculação mecânica. A sobrevivência do PLYV em folhas e raízes secas de mamoeiro infetado foi avaliada por sorologia e inoculação em plantas sadias. A presença do vírus foi detectada em folhas e raízes de mamoeiro secas, indicando que o vírus pode permanecer ativo em restos de cultura por até 120 dias. A purificação do PLYV permitiu a obtenção de 309.5 mg de vírus/kg de folha e o anti-soro obtido mostrou-se altamente específico, com títulos de 1:128 em dupla difusão em Agar e 1:1.024.000 em Elisa indireto. Estudos das propriedades físicas do PLYV em mamoeiro revelaram um ponto de inativação térmica (PIT em torno de 80 ºC, um ponto máximo de diluição (PMD de ac. 10-6 e uma longevidade in vitro (LIV acima de 50 dias.The Papaya lethal yellowing virus (PLYV is responsible for one of the most important disease of papaya (Carica papaya in the Northeast of Brazil. The PLYV can be transmitted through the soil, irrigation water, agriculture tools and contaminated hands. The present research had the objective to characterize biologically, serologically and physically a PLYV isolate and evaluates how long it survives in infected dried tissues. The PLYV was identified by indirect enzyme linked immunoabsorbent assay (ELISA, isolated in young papaya plants by mechanical inoculations and maintained at green house conditions for virus purification. Approximately 309.5 mg

  20. Tradición, (retradución e reformulación na General Estoria e na Estoria de Troya afonsinas á luz dun testemuño indirecto do séc. XIV

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    Ricardo Pichel Gotérrez

    2012-07-01

    Full Text Available O processo de conformação da General Estoria, nomeadamente da Estoria de Troya integrada na segunda parte da compilação afonsina, pode-se rastejar a partir de dois testemunhos indirectos do século XIV: o ms. 10.146 da Biblioteca Nacional de Madrid (que transmite um dos testemunhos da Historia Troyana Polimétrica e o ms. 558 da Biblioteca de Menéndez Pelayo de Santander (a Historia Troiana “bilingue”. O presente trabalho centra-se neste último exemplar, um manuscrito híbrido inicialmente redigido em castelhano sob o patrocínio de Pedro I de Castela (ca. 1365-1369, mas que pouco tempo depois iria ser completado em galego no seio da corte nobiliária corunhesa dos Andrade (ca. 1369-1373. Através de uma análise parcial da interpolação da General Estoria (especialmente GE1 e GE2 contida neste testemunho, oferecemos várias conclusões (de caráter discursivo e ideológico vinculadas com o processo de compilatio e ordinatio de uma das grandes “estorias unadas” da grão crónica universal afonsina, dado que o texto transmitido pola Historia Troiana reflecte um estádio discursivo anterior dos materiais troianos que finalmente teriam chegado a se compendiar na obra de Afonso X.Le processus de conformation de la General Estoria, plus précisément de l’Estoria de Troya intégrée dans la deuxième partie de la compilation alphonsine, peut être recherché à partir de deux témoignages manuscrits indirects du XIVe siècle : le ms. 10.146 de la Bibliothèque Nationale de Madrid (qui transmet l’un des témoignages de l’Historia Troyana Polimétrica et le ms. 558 de la Bibliothèque de Menéndez Pelayo de Santander (l’Historia Troiana “bilingue”. L’article présenté se concentre sur ce dernier exemplaire, un manuscrit hybride rédigé en castillan d’abord sous les auspices du roi Pedro I de Castille (ca. 1365-1369, mais qui fut complété peu de temps après en galicien au sein de la cour nobiliaire des Andrade

  1. Malformaciones venosas orofaciales de bajo flujo: esclerosis endoluminal con láser de diodo Low-flow orofacial venous malformations: endoluminal sclerosis with a diode laser

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    M. Puche Torres

    2010-06-01

    Full Text Available Introducción: Las anomalías vasculares son procesos frecuentes que se localizan en más del 50% de los casos en la región de la cabeza y el cuello. Las técnicas clásicas de tratamiento, tales como la cirugía y la esclerosis química, han dado paso a modernas técnicas menos invasivas, tales como el láser Nd:YAG. Por otra parte, se ha utilizado con éxito el láser de diodo (980 nm para el tratamiento de varices mediante esclerosis endoluminal. Nuestra propuesta es la utilización del láser de diodo (l 980 nm para provocar una esclerosis por fotocoagulación intralesional de las malformaciones venosas de bajo flujo dada la capacidad de este láser de ser transmitido por fibra óptica. Objetivo: Exponer nuestros resultados en el tratamiento de malformaciones venosas de bajo flujo (MVBF orofaciales mediante la terapéutica de esclerosis endoluminal con láser de diodo. Material y métodos: Revisamos 84 pacientes que presentaban MVBF orofaciales tratados con láser de diodo. Describimos la técnica de realización y se muestran los resultados postoperatorios a corto plazo. Resultados: Tras un periodo de seguimiento no inferior a 12 meses se constató curación en el 95,24% aplicando una o dos sesiones y solamente en 4 casos se objetivó recidiva. Concluimos que la técnica de esclerosis endoluminal con láser de diodo de MVBF en el área orofacial se constituye como una nueva técnica de tratamiento, mínimamente invasiva, ambulatoria, y que permite la resolución de los casos sin tener que recurrir a cirugías más agresivas y con excelentes resultados funcionales y estéticos.Background: Vascular anomalies are common processes that involve the head and neck region in more than 50% of the cases. Traditional treatment options such as surgery and chemical sclerosis have given way to modern less-invasive techniques, including Nd:YAG laser treatment. On the other hand, 980 nm laser diode has been successfully used for the endovenous sclerosis

  2. Serosurvey for tick-borne diseases in dogs from the Eastern Amazon, Brazil Pesquisa Sorológica por doenças transmitidas por carrapatos em cães da Amazônia oriental, Brasil

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    Mariana Granziera Spolidorio

    maior (40,3% do que os cães urbanos (21,1%. Esse primeiro estudo sobre agentes transmitidos por carrapatos entre cães da Amazônia oriental brasileira indica que estes animais estão expostos a vários agentes. Estes incluem Babesia principalmente na área urbana, Riquétsias do grupo da Febre Maculosa principalmente nas áreas rurais, e Erliquia em cães de ambas as áreas, rural e urbana.

  3. Transmissão de Alternaria spp. através de sementes de feijão e seu efeito sobre a qualidade fisiológica das sementes Transmission of Alternaria spp. by common bean seeds and its effects on physiological quality

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    Maria Heloisa Duarte de Moraes

    2006-09-01

    Full Text Available Sementes de feijão podem transportar grande número de patógenos que têm a capacidade de afetar sua qualidade fisiológica ou causar doenças em diferentes estádios da cultura, afetando seu rendimento. O agente causal da mancha de Alternária do feijoeiro é um desses patógenos, porém não existem muitos estudos avaliando a transmissão de diferentes espécies de Alternaria associadas a essa doença e seu efeito sobre a qualidade fisiológica. Sendo assim, desenvolveu-se este trabalho com o objetivo de verificar a transmissão de espécies de Alternaria através das sementes de feijão e seu efeito sobre a germinação das mesmas e sobre a emergência de plântulas em casa de vegetação. Isolados de Alternaria alternata, A. carthami e A. cichorii foram inoculados em sementes de feijão e estas foram submetidas aos testes de sanidade, germinação e emergência de plântulas em casa de vegetação. A inoculação com os isolados de A. alternata proporcionou sementes com quase 100% de incidência; os resultados obtidos com os demais isolados variaram de zero, para os isolados 3 (A. cichorii e 8 e 9 (A. carthami, até 75% para o isolado 7 (A. carthami. A maioria dos isolados foi transmitida para as plântulas, sendo os isolados 1 (Alternaria cichorii e 4, 5, 8, 9 e 11 (A. carthami transmitidos em maior porcentagem. Não se constatou efeito dos isolados sobre a emergência das plântulas e não houve correlação positiva entre a porcentagem de incidência do fungo e a porcentagem de plântulas infectadas. Alguns isolados de A. carthami causaram queda na germinação das sementes.A great number of pathogens can be transported by seeds; they had capacity to affect physiological quality or to cause diseases in many stages of the culture, reducing yield. One of these pathogens is the causal agent of Alternaria leaf spot. There aren't many researches about seed transmission of distinct Alternaria species and its effects on common bean

  4. Composition and Bioactive Properties of Yerba Mate (llex paraguariensis A. St.-Hil.: A Review Composición y Propiedades Bioactivas de la Yerba Mate (Ilex paraguariensis A. St.-Hil.: Una Revisión

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    Kellie P Burris

    2012-06-01

    la yerba mate se ha cultivado y consumido por siglos en América del Sur, su popularidad en los Estados Unidos ha aumentando debido a la demanda por bebidas saludables y alimentos más naturales y por los potenciales beneficios para la salud de la yerba mate (antioxidante, antimicrobiano, acción contra la obesidad y diabetes, digestivo, estimulante. La yerba mate también se ha investigado como agente de prevención y causa de algunos tipos de cáncer, causando controversia entre investigadores. Investigaciones recientes han ampliado el espectro de uso de la yerba mate como agente antimicrobiano, protección de cultivos y acción contra patógenos transmitidos por alimentos. Resultados prometedores para el uso de esta planta en la salud humana y animal han llevado a esta revisión. Esta revisión se centró en la composición de la yerba mate, y el efecto que el cultivo y el procesamiento puede tener sobre sus propiedades.

  5. LACTANCIA MATERNA: Una revisión de la evaluación de esta práctica a través del tiempo. El caso de algunas comunidades indígenas en Colombia Breastfeeding: a review of its evolution through time. The case of some indigenous communities in Colombia

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    Fabiola Becerra Bulla

    2009-01-01

    Full Text Available La lactancia materna es una función innata de todo mamífero y el hombre como tal no es la excepción; el presente artículo se basa en una revisión de fuentes secundarias relacionadas con la evolución histórica de la práctica de la lactancia materna en diferentes contextos y momentos históricos. Realiza una aproximación a la información de tipo histórico que existe sobre dicha práctica, desde la civilización egipcia y en la Europa antes de Cristo, hasta el siglo XX, haciendo mención a las evidencias sobre los conocimientos y creencias en torno a la lactancia materna. Posteriormente se presenta un panorama del contexto colombiano desde la preconquista, la Colonia y la Independencia, hasta nuestros días; no obstante, es preciso y de gran importancia resaltar la continuidad de esta práctica bajo la influencia de la cultura indígena colombiana, motivo por el cual al final del mismo se señalan las características de las prácticas de la alimentación al seno y alimentación complementaria en algunas comunidades indígenas colombianas. Se parte del hecho de que la alimentación al pecho ha sido durante centenares de años una práctica eminentemente biocultural porque, además de ser un proceso biológico, es un comportamiento determinado por la cultura el cual sido transmitido de madres a hijas a través de generaciones.Breastfeeding is an innate function of all mammalians, and humans are not an exception. This article reviews secondary sources related to the evolution of breastfeeding in different contexts and historical moments. Thus, breastfeeding has been since centuries ago an eminently biocultural practice because, besides being a biological process, it is a behavior determined by culture that has been transmitted from mother to daughters through generations. This article carries out an approximation to hystorical information about this practice, since the Egyptian civilization and in Europe before Christ until the twentieth

  6. Transmissão experimental do Grapevine virus B pela cochonilha Pseudococcus longispinus Targioni-Tozzetti (Hemiptera: Pseudococcidae Transmission of the Grapevine virus B by the mealybug Pseudococcus longispinus Targioni-Tozzetti (Hemiptera:Pseudococcidae in Brazil

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    Hugo Kuniyuki

    2006-06-01

    Full Text Available Em São Paulo, existem dois isolados do Grapevine virus B (GVB, sorologicamente semelhantes e sintomatologicamente distintos, que causam a doença denominada fendilhamento cortical ("grapevine corky bark", GCB. Na literatura estrangeira existem relatos de que o GVB pode ser transmitido por cochonilhas brancas. O objetivo do presente trabalho foi o de verificar a transmissibilidade do GVB de videira infectada para videira sadia através da cochonilha da espécie Pseudococcus longispinus. Os dois isolados do vírus foram testados: o isolado comum (GVB-C e o isolado Itália (GVB-I. A confirmação de infecção foi feita através da análise visual de sintomas, ELISA e RT-PCR. Em todos os testes de inoculação experimental, os primeiros sintomas da virose foram notados com, aproximadamente, 8 a 12 meses após a exposição às cochonilhas. Plantas sadias da variedade LN-33, mantidas ao redor de uma planta infectada com o GVB-C e altamente infestada pela P. longispinus, tornaram-se infectadas com incidência de 54,2%, após 4 anos. Empregando-se inoculação experimental com cochonilhas virulíferas, plantas da indicadora LN-33 apresentaram infecção de 46,2% e 40,0% para o GVB-C e GVB-I, respectivamente, após 3 anos de observações. Apesar desta espéciede cochonilhaocorrer de maneira eventual nos vinhedos do Estado de São Paulo, precauções devem ser tomadas em áreas onde são mantidos clones sadios de variedades de copa e de porta-enxerto de videira, visto que esses insetos, além de possuírem grande número de plantas hospedeiras, também podem transmitir outros importantes vírus da videira.In the State of São Paulo, Brazil, there are two isolates of Grapevine virus B (GVB associated with grapevine corky bark disease (GCB. Although serologically similar, they induce distinct reaction on some grape varieties. They are called GVB-C for common isolate and GVB-I for isolate obtained from the variety Italia. The objective of this work was

  7. Bioprotetores e fungicidas químicos no tratamento de sementes de soja Bioprotectors and chemical fungicides in the treatment of soybean seeds

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    Liliane Marcia Mertz

    2009-02-01

    Full Text Available Na cultura da soja existem, diversos patógenos que causam prejuízos à qualidade das sementes. O tratamento de sementes oferece garantia adicional ao estabelecimento da lavoura a custos reduzidos, sendo utilizado, principalmente, com a finalidade de permitir a germinação de sementes infectadas, controlar patógenos transmitidos pela semente e proteger a semente dos fungos do solo. O aumento significativo do tratamento de sementes de soja com fungicidas demanda soluções alternativas, como o uso de bioprotetores, no intuito de reduzir a utilização de pesticidas sintéticos, os riscos aos operadores e os possíveis prejuízos a inoculação com bactérias do gênero Bradyrhizobium. O objetivo deste trabalho foi avaliar a eficácia do tratamento de sementes de soja com bioprotetores e fungicidas químicos na sanidade de sementes e emergência de plantas. Foram testados os agentes biológicos Biotrich (Trichoderma spp., HFF turfa fértil e Bacillus pumilus e os fungicidas químicos carbendazin + thiram, carboxin + thiram e difenoconazole + metalaxyl. A avaliação do desempenho dos produtos foi realizada em laboratório, onde foi avaliada a sanidade das sementes por meio do teste de papel filtro; casa-de-vegetação, onde foi avaliava a emergência em areia e, em campo, onde foi avaliada a emergência e a estatura de plantas. De acordo com os resultados deste trabalho, os fungicidas químicos, especialmente do grupo dos benzimidazóis associados a um fungicida de contato (thiram, garantem um estande adequado de plantas, mesmo com a semeadura coincidindo com períodos de estiagem. Já o tratamento de sementes de soja com protetores biológicos, não oferece proteção às sementes no solo, causando redução acentuada na germinação e emergência de plantas.Several pathogens can damage the soybean seed quality. Seeds treatment offers additional assurance to the establishment of farming at reduced costs, used mainly with the purpose of

  8. Evolução fatal da co-infecção doença de Chagas/Aids: dificuldades diagnósticas entre a reagudização da miocardite e a miocardiopatia chagásica crônica Fatal evolution of Chagas'disease/Aids co-infection: diagnostic difficulties between myocarditis reactivation and chronic chagasic myocardiopathy

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    Eros Antonio de Almeida

    2009-04-01

    Full Text Available A doença de Chagas é uma parasitose causada pelo protozoário Trypanosoma cruzi, transmitido por insetos triatomíneos. A doença ocorre desde o sul dos Estados Unidos da América do Norte até a Argentina, sendo que, aproximadamente, 14 milhões de pessoas devam estar infectados na América Latina, predominantemente na forma crônica da doença. A reagudização da doença de Chagas pode ocorrer em imunossuprimidos, como tem sido observado em pacientes com aids. Verificou-se descompensação cardíaca em um destes casos, com grave disfunção ventricular e arritmias sendo considerada a possibilidade de reagudização da doença de Chagas no miocárdio, uma vez que o xenodiagnóstico foi positivo. Face a gravidade foi tratado especificamente para o Trypanosoma cruzi com benznidazol, porém sem completar o tempo estipulado para este fim, vindo a falecer em conseqüência de complicações da cardiopatia. A necropsia apresentou os estigmas habituais da cardiopatia chagásica crônica como miocardite fibrosante e redução do número de neurônios no tubo digestório, não sendo encontradas formas amastigotas do Trypanosoma cruzi em nenhum dos tecidos examinados. Assim, não ficou demonstrada a reagudização da doença de Chagas, mas sim evolução natural da cardiopatia chagásica crônica.Chagas disease is a type of parasitosis caused by the protozoan Trypanosoma cruzi, and it is transmitted by triatomine insects. This disease is found between the southern United States to Argentina and approximately 14 million people in Latin America are believed to be infected, predominantly with the chronic form of the disease. Reactivation of Chagas disease can occur among immunosuppressed patients, as has been observed among AIDS patients. In one such case, we observed cardiac decompensation with severe ventricular dysfunction and arrhythmias. This case was thought to be reactivation of Chagas disease in the myocardium, since the xenodiagnosis was

  9. Rol de los cereales de invierno y su sistema de manejo en la dinámica poblacional de Delphacodes kuscheli, insecto vector del MRCV Role of winter cereals and their management system in the population dynamics of Delphacodes kuscheli, the MRCV vector insect

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    G.T. Boito

    2008-06-01

    Full Text Available El Mal de Río Cuarto es la enfermedad virósica más importante del maíz en la Argentina. El virus pertenece al género Fijivirus (Reoviridae. Es transmitido en forma persistente propagativa por Delphacodes kuscheli Fennah (Hemíptera- Delphacidae. Varios cultivos son reservorios naturales del virus y hospedantes del vector. El objetivo de este trabajo fue determinar el rol de la avena, cebada, centeno, triticale y trigo (pastoreados y no pastoreados en la dinámica poblacional de D. kuscheli. El ensayo se realizó dentro del área endémica de la enfermedad, bajo un diseño en franjas en 1998, 1999 y 2000. Se estudió la variación del número de individuos en el tiempo, utilizando como unidad de muestreo los individuos colectados semanalmente en cien golpes de red. Éstos fueron clasificados en ninfas y adultos (macrópteros y braquípteros. Los resultados muestran la importancia del manejo sobre las poblaciones del vector, ya que éstas son mayores en los cultivos pastoreados. La avena registró el mayor número de individuos, seguida por trigo y cebada. El momento del pico poblacional de adultos macrópteros en avena es diferente a los otros cultivos evaluados. La tasa de crecimiento poblacional de ninfas y adultos braquípteros fue mayor en avena, mientras que no se registraron diferencias entre los otros cultivos.The Mal de Río Cuarto is the most important disease in maize in Argentina. The virus belongs to the Genus Fijivirus (Reoviridae. It is transmitted in a persistent propagative manner by Delphacodes kuscheli Fennah (Hemíptera- Delphacidae. Several grain crops are virus reservoirs and insect vector hosts. The objective of this work was to study the role of grazed and non grazed oat, barley, wheat, rye and triticale in the population dynamics of Delphacodes kuscheli. The experimental plots were sown in the endemic MRC area under a strip design during the 1998, 1999 and 2000 seasons. Vector insect populations were evaluated weekly

  10. O que a saúde tem a ver com rádio comunitária?: uma análise de uma experiência em Nova Friburgo - RJ What does health have to do with community radio?: an analysis of an experience in Nova Friburgo - State of Rio de Janeiro

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    Alfredo de Oliveira Neto

    2013-02-01

    Full Text Available O campo da Comunicação e Saúde no Brasil vem se desenvolvendo e se fortalecendo a cada Conferência Nacional de Saúde (CNS. No relatório final da XII CNS, em 2003, torna-se claro o reconhecimento das rádios comunitárias como instrumento de divulgação e produção de temas relacionados ao SUS. O objetivo foi analisar as relações que se estabelecem entre profissionais de saúde, ouvintes/usuários e comunicadores envolvidos com uma rádio comunitária, de modo a entender como são constituídos os nexos entre um programa de rádio sobre saúde e os imaginários desses sujeitos. Para tal, uma abordagem metodológica qualitativa, fazendo-se uso da etnografia e do estudo de recepção. O campo se constituiu de um programa sobre saúde, o Bloco Mulher Saúde, transmitido pela rádio comunitária Rádio Comunidade FM 104,9 no município de Nova Friburgo, RJ. As discussões foram divididas em categorias analíticas. A comunicação comunitária pode contribuir como mediador político-cultural ampliando as possibilidades de expressão das demandas sobre saúde; existe a manutenção e reprodução do linguajar técnico hegemônico em saúde pelos médicos ao participarem de uma rádio comunitária; a comunicação comunitária pode auxiliar na construção de estratégias para ampliar o controle social no SUS.The field of Communication and Health in Brazil has been developing and getting stronger after each National Health Conference (NHC. In the final report of the XII NHC, in 2003, there was clear recognition that community radio is an instrument for the dissemination and treatment of issues related to the Brazilian Unified Health System (SUS. This study seeks to analyze the relationships that are established between health professionals, listeners/users and popular communicators as a means of understanding the nexus between a radio program on health and the imaginations of the listeners. A qualitative methodological approach was used of

  11. Boletim Informativo em UTI: percepção de familiares e profissionais de saúde

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    Edna Magalhães de Alencar Barbosa

    2008-03-01

    Full Text Available No cotidiano da Unidade de Terapia Intensiva (UTI a família mostra-se ansiosa, tem medo da perda e do desconhecido. Essa situação é mais crítica quando se depara com uma unidade de acesso limitado por horários rígidos e curto tempo de visita por paciente. Dentre as normas e rotinas das Unidades de Terapia Intensiva (UTI, encontra-se aquela relativa às informações clínicas sobre os pacientes internados, que geralmente são fornecidas por meio de um Boletim Informativo (BI, contendo termos como: grave, estável, regular, que não esclarecem satisfatoriamente o estado do familiar internado. É comum a família ter acesso ao Informativo e buscar informações adicionais por meio de telefonemas ou pessoalmente na UTI. Estudo descritivo exploratório que objetivou identificar semelhanças e diferenças entre Boletins Informativos (BI de Unidades de Terapia Intensiva (UTI; descrever a percepção de familiares e profissionais sobre os BI como instrumento de comunicação do estado do paciente nas UTI; identificar os aspectos comunicacionais envolvidos na compreensão dos BI das UTI; identificar sugestões que possibilitem elaborar um informativo adequado às necessidades dos familiares. Foram realizadas entrevistas semi-estruturadas com 41 familiares e profissionais, e as respostas analisadas conforme propõe Bardin (2004. Evidenciou-se que os BI são semelhantes na forma, conteúdo e maneira de transmitir as informações. A maioria dos informantes caracterizou o BI como pouco compreensível e de linguagem técnica, que não esclarece a situação do paciente, gerando confusão e dúvidas sobre a confiabilidade das informações. Consideraram-no subjetivo e controverso pela falta de critérios usados na sua elaboração, mas que ainda é uma forma eficaz de transmitir informações. Sugeriram inclusão de itens menos técnicos e um glossário anexo, transmitido por profissional capacitado. Identificou-se similaridade nos discursos dos

  12. As deficiências auditivas relacionadas às alterações do DNA mitocondrial. Hearing loss related to mitochondrial DNA changes

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    Maria F. P. de Carvalho

    2002-03-01

    Full Text Available A deficiência auditiva é sintoma comum que pode apresentar várias etiologias, entre elas as causadas por alterações genéticas. As mutações genéticas podem ocorrer em genes nucleares e mitocondriais. A mitocôndria, uma organela intracelular, tem o seu próprio genoma (DNA, que é uma molécula circular e é transmitido exclusivamente pela mãe. As mutações do DNA mitocondrial são transmitidas pela linhagem materna, mas podem ocorrer mutações espontâneas. O fenótipo, ou expressão clínica, da mutação mitocondrial vai depender da quantidade de DNA mitocondrial mutante existente na célula, situação conhecida como heteroplasmia. A mitocôndria tem a função de disponibilizar energia para as células sob a forma de ATP (trifosfato de adenosina. Os órgãos que requerem grande quantidade de energia são mais comumente acometidos em casos de mutações do DNA mitocondrial, como células nervosas, musculares, endócrinas, ópticas e auditivas. Como a cóclea é grande consumidora de energia, uma mutação no DNA mitocondrial de células ciliadas causa deficiência auditiva do tipo neurossensorial, bilateral, simétrica e progressiva. As deficiências auditivas causadas por mutações no DNA mitocondrial correspondem a 0,5% a 1% de todas as deficiências auditivas de origem genética. Foi realizada uma extensa revisão bibliográfica, a fim de estudar as deficiências auditivas causadas por alterações no DNA mitocondrial. A deficiência auditiva pode se apresentar na forma isolada (forma não sindrômica, como nos casos de hiper-sensibilidade aos antibióticos aminoglicosídeos e presbiacusia, ou associada a outras doenças (forma sindrômica, como na síndrome de Kearns-Sayre e diabete e surdez de herança materna.Hearing loss is a common symptom that may be manifested by many etiologies and it is frequently associated to genetic problems. Genetic mutations may occur in nuclear or mitochondrial genes. Mitochondria are

  13. O terramoto de Lisboa de 1755: tremores e temores

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    María José Ferro TAVARES

    2009-11-01

    Full Text Available RESUMEN: Con anterioridad al gran terremoto de 1755 ya se habían registrado en Portugal temblores de tierra de intensidad bastante significativa. En la actualidad, las cartas sismotectónicas enseñan que tanto la región de la Gran Lisboa, como la costa sur atlántica y costa algarvia son las que presentan mayor peligrosidad sísmica. Los temblores de tierra fueron desde siempre un asunto de preocupación para la población portuguesa que habitaba estas regiones, provocando temores y fobias que fueron transmitidos de generación en generación. En el presente trabajo se procura identificar las señales de cambios o de continuidad en la población portuguesa y las reacciones ante los efectos de los terremotos que desde tiempos remotos afectaran al país. Particular atención es atribuida a las interpretaciones de raíz escatológica, las primeras que surgieron y que gradualmente fueran sustituidas en el siglo XVII por explicaciones que invocaban la existencia de causas primeras (divinas y causas segundas (naturales en la discusión de este tipo de fenómenos. Va a ser con el terremoto de Lisboa, en 1755, cuando la experimentación surge como medio de explicación, inicialmente apenas en términos retóricos, para después, a partir del siglo XIX, en términos científicos, sustentar la aparición y desarrollo de la propia sismología. En Portugal, mientras exista un copioso acervo bibliográfico en lo que concierne a las referidas explicaciones, una actitud pragmática ante los terremotos ha prevalecido siempre, en el sentido de que es siempre más importante mitigar sus efectos, particularmente los efectos en la salud pública en lo que se refiere al terremoto de Lisboa, que determinar sus causas exactas.ABSTRACT: Before the great Lisbon earthquake of 1755 the continental part of Portugal had already experienced strong, catastrophic tremors; present day seismotectonic maps show that indeed the metropolitan area of the capital city, as well as

  14. Comparação de três receptores GPS para uso em agricultura de precisão Comparison of three GPS receiver for precision agriculture uses

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    Marcelo C. C. Stabile

    2006-04-01

    Full Text Available Diversos equipamentos que se utilizam dos sinais transmitidos pelo Sistema de Posicionamento Global (GPS têm sido empregados na Agricultura de Precisão. Neste estudo, foi feita uma comparação de três receptores comerciais no intuito de verificar suas acurácias. As principais qualidades medidas foram a repetibilidade dos dados e a estabilidade do sistema. O estudo foi conduzido em campo aberto, na área experimental da ESALQ/USP, com três linhas paralelas de 50 m espaçadas 10 m entre si. A coleta de dados foi feita no dia 25-9-2002, totalizando seis repetições da comparação dos três sistemas. Os dados foram coletados simultaneamente, de acordo com a melhor configuração da constelação de satélites, sendo que os três sistemas apresentaram resultados distintos. O GPS "A" (oito canais, sem correção diferencial apresentou a maior variabilidade e a menor repetibilidade, com desvios de quase 10 m numa mesma linha. O sistema "B", com sinal de correção diferencial (DGPS "B", 12 canais, apresentou acurácia superior ao "A", com desvios de, no máximo, 6 m, mas com repetibilidade significativa. O GPS "C" (12 canais, com algoritmo otimizado apresentou a maior acurácia e desvio máximo de 2 m. Assim, os dois últimos sistemas são adequados para a aplicação dos conceitos da Agricultura de Precisão.Several brands of equipment have been used with global positioning systems (GPS in Precision Agriculture. The quality of these different receivers varies according to their capabilities in terms of accuracy and repeatability. In this study, three commercial systems were tested to compare their accuracies. This study focused on measuring the stability of the receiver and the repeatability of data. The study was conducted in the experimental area of ESALQ/USP. It consisted of three parallel 50-meter lines spaced 10 meters apart. Data collection was done on 9-25-2002 with a total of six replications. Data was collected simultaneously and the

  15. Pathogenic microorganisms survival in ambrosiaSobrevivência de micro-organismos patogênicos em ambrósia

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    Cláudio Dias Timm

    2013-09-01

    Full Text Available Ambrosia is a kind of dulce de leche homemade with milk, eggs and sugar. It is usually sold in free markets and it is largely consumed in South America. Food contamination by pathogenic microorganisms can occur during the food processing, in distribution centers, in retail markets or in the consumer’s homes. The aim of this study was to evaluate the survival in ambrosia of main pathogenic microorganisms eventually transmitted by dairy products. Ambrosia fractions were experimentally contaminated with Salmonella enterica subsp. enterica serotype Typhimurium, Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus. Analysis to evaluate the microorganisms’ viability were made after storage for 0, 1, 2, 3, 5, 10, 20 and 30 days. Salmonella and L. monocytogenes were recovered from all samples during the 30 days of study. E. coli O157:H7 was isolated until the tenth day and S. aureus until the third day. It was demonstrated that important pathogenic microorganisms are able to survive up to 30 days in ambrosia, which makes this product a potential carrier of food-borne diseases. This work is the first study about the possibility of ambrosia transmitting relevant public-health danger pathogenic microorganisms. Ambrosia é um tipo de doce de leite preparado artesanalmente com leite, ovos e açúcar, comumente comercializado em feiras livres e muito consumido na América do Sul. A contaminação de alimentos por microrganismos patogênicos pode ocorrer durante as etapas de processamento, nos centros de distribuição, no mercado varejista ou na casa do consumidor. O trabalho teve como objetivo avaliar a sobrevivência em ambrosia dos principais microrganismos patogênicos eventualmente transmitidos por leite e derivados. Alíquotas de ambrosia foram experimentalmente contaminadas com Salmonella enterica subsp. enterica sorotipo Typhimurium, Escherichia coli O157:H7, Listeria monocytogenes e Staphylococcus aureus. Foram realizadas

  16. Desempenho de rede de sensores sem fio em casa de vegetação Performance of wireless sensor network in a greenhouse

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    Barbara Teruel

    2012-12-01

    Full Text Available A potência de sinal é um importante parâmetro a ser considerado em um projeto de implantação de rede de sensores sem fio no meio agrícola. Este trabalho tem como objetivo coletar dados de qualidade de sinal transmitido entre uma estação base e um nó sensor situado no interior de uma casa de vegetação, localizada no campo experimental da Faculdade de Engenharia Agrícola - UNICAMP, com o auxílio de ferramentas computacionais. Após a implantação do nó sensor foram realizados experimentos com vista a se verificar alcance e qualidade do sinal de acordo com sua potência. Através desses ensaios foi possível concluir que uma potência de sinal com valor de -12,2 dBm é suficiente para manter a transmissão entre a estação base e nó sensor. Testes também foram realizados com uma potência de sinal de -9,2 dBm e mostraram que a rede possuía qualidade classificada "muito alta" porém, através do teste de duração da bateria, nota-se que existe economia de energia ao se utilizar a potência de sinal de -12,2 dBm. Foi avaliada a influência que o valor da potência de sinal tem sobre a qualidade do sinal de transmissão e recepção, o gasto de bateria e o alcance do sinal.The signal strength is an important parameter to be considered in a project to establish network of wireless sensors in an agricultural environment. This work aims to collect quality data signals transmitted between a base station and a sensor node located inside a greenhouse, which is an experimental field located in the College of Agricultural Engineering - UNICAMP, with the help of computational tools. After sensor node deployment, experiments were performed in order to check range and signal quality. Through these experiments, it was concluded that a radiofrequency power value of -12.2 dBm is sufficient to maintain transmissions between base station and the sensor. Tests were also performed to a radiofrequency power of -9.2 dBm, and showed that the network

  17. Sorologia positiva para sífilis, toxoplasmose e doença de Chagas em gestantes de primeira consulta em centros de saúde de área metropolitana, Brasil Positive sorology of syphilis, toxoplasmosis and Chagas' disease in pregnant women on their first visit to State Health Centes in a metropolitan area, Brazil

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    Adelaide José Vaz

    1990-10-01

    Full Text Available Alguns testes sorológicos têm sido utilizados para detectar, indiretamente, a presença de possíveis agentes etiológicos infecciosos durante a gestação, que sendo transmitidos ao feto, por via placentária, causam infecções congênitas com seqüelas leves ou graves e até morte fetal. Foram estudadas 481 gestantes, com idade média de 24,5 anos (de 14 a 46 anos, atendidas em primeira consulta nos Centros de Saúde, na Cidade de São Paulo, SP (Brasil no período de abril a outubro de 1988. Segundo o trimestre gestacional, 230 pacientes (47,8% estavam no primeiro trimestre; 203 (42,2% no 2º e 48 (10,0% no 3º. Das 474 pacientes que declararam algum tipo de renda mensal, 309 (65,2% pertenciam a familias com renda até 1 SMPC (salário mínimo per capita e somente 15 (3,2% pertenciam a famílias com renda superior a 3 SMPC, caracterizando o baixo nível econômico das gestantes. Das 481 pacientes 159 (33,1% eram nascidas no Estado de São Paulo e as 322 (66,9% restantes eram imigrantes procedentes de outros Estados, destacando-se Bahia (23,1%; Minas Gerais (11,4%; Paraná (7,5%; Paraíba (5,4% e Pernambuco(5,4%. Foram realizados testes imunodiagnósticos para sífilis, toxoplasmose e doença de Chagas. Foram observados resultados positivos para sífilis em 25 gestantes (5,2%. Para toxoplasmose, 157 (32,4% não tinham anticorpos em nível detectável e 67 (13,9% apresentaram títulos elevados, indicativos de infecção ativa, das quais em 6 (10,3% foram detectados anticorpos da classe IgM. Para doença de Chagas foram encontrados anticorpos específicos em 14 (2,9% gestantes, sendo que destas, 10 (71,4% eram procedentes da Bahia e Minas Gerais.The diagnosis of some infectious diseases contracted during pregnancy is very important, as these diseases can be transmitted to the fetus. Four hundred and eighty-one pregnant women were studied, of an average 24.5 years of age (from 14 to 46, during their first prenatal visit to the State of S

  18. Aula virtual y presencial en aprendizaje de comunicación audiovisual y educación Virtual and Real Classroom in Learning Audiovisual Communication and Education

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    Josefina Santibáñez Velilla

    2010-10-01

    Full Text Available El modelo mixto de enseñanza-aprendizaje pretende utilizar las tecnologías de la información y de la comunicación (TIC para garantizar una formación más ajustada al Espacio Europeo de Educación Superior (EEES. Se formularon los siguientes objetivos de investigación: Averiguar la valoración que hacen los alumnos de Magisterio del aula virtual WebCT como apoyo a la docencia presencial, y conocer las ventajas del uso de la WebCT y de las TIC por los alumnos en el estudio de caso: «Valores y contravalores transmitidos por series televisivas visionadas por niños y adolescentes». La investigación se realizó con una muestra de 205 alumnos de la Universidad de La Rioja que cursaban la asignatura de «Tecnologías aplicadas a la Educación». Para la descripción objetiva, sistemática y cuantitativa del contenido manifiesto de los documentos se ha utilizado la técnica de análisis de contenido cualitativa y cuantitativa. Los resultados obtenidos demuestran que las herramientas de comunicación, contenidos y evaluación son valoradas favorablemente por los alumnos. Se llega a la conclusión de que la WebCT y las TIC constituyen un apoyo a la innovación metodológica del EEES basada en el aprendizaje centrado en el alumno. Los alumnos evidencian su competencia audiovisual en los ámbitos de análisis de valores y de expresión a través de documentos audiovisuales en formatos multimedia. Dichos alumnos aportan un nuevo sentido innovador y creativo al uso docente de series televisivas.The mixed model of Teaching-Learning intends to use Information and Communication Technologies (ICTs to guarantee an education more adjusted to European Space for Higher Education (ESHE. The following research objectives were formulated: 1 To find out the assessment made by teacher-training college students of the virtual classroom WebCT as an aid to face-to-face teaching. 2 To know the advantages of the use of WebCT and ICTs by students in the case study:

  19. Impactos econômicos da introdução do milho Bt11 no Brasil: uma abordagem de equilíbrio geral inter-regional

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    Andressa Rodrigues Pavão

    2011-03-01

    Full Text Available Este trabalho analisa os impactos econômicos da adoção do milho Bt11 no Brasil, bem como as consequências da proibição deste cultivo apenas no Paraná, caso a lei estadual nº 14.162/03 entrasse em vigor. Para tal, utiliza-se um modelo computável de equilíbrio geral inter-regional, calibrado para 2001, simulando a redução de inseticida, mão de obra, combustível e lubrificantes, bem como o aumento de produtividade observado em lavouras de milho Bt11. Ao analisar a adoção nas regiões brasileiras "tecnificadas", observa-se o deslocamento de estoque de capital e mão de obra de todas as regiões para o Sul do País. Considerando-se que apenas o Paraná não adota milho Bt11, observa-se que tanto a mão de obra quanto o estoque de capital se deslocam do Sul e Sudeste para o Centro-Oeste do Brasil. Os resultados mais expressivos ocorrem no próprio estado do Paraná, onde não apenas o setor de milho, como também os setores a jusante perdem competitividade, reduzindo o nível de atividade, emprego e consumo das famílias. De forma geral, os efeitos da adoção do milho Bt11 são transmitidos ao longo da sua cadeia produtiva, gerando aumento do PIB, das exportações e do consumo das famílias, sendo mais expressivos nos setores e regiões diretamente relacionados com a cadeia de comercialização do milho, tais como os de criação animal e carnes, localizados, em sua maioria, no Sul do País.This study aims to analyze the economic impacts of the adoption of Bt11 corn in Brazil, as well as the consequences of the prohibition of the cultivation only in the Paraná state, if the state law number 14.162/03 would start to be active. For this purpose, an inter-regional general equilibrium computable model is used, gauged for 2001, simulating the reduction of insecticide, labor force, fuel and lubricants, as well as the increase in the yield observed in crops which use Bt11 corn. When the adoption of Bt11 corn in "technified" Brazilian

  20. Número mais provável de Salmonella isoladas de carcaças de frango resfriadas Most probable number of Salmonella isolated from refrigerated broiler carcasses

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    Anderlise Borsoi

    2010-11-01

    Full Text Available A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analisadas 180 carcaças de frangos resfriadas, adquiridas em varejos, para pesquisa de Salmonella com determinação do número de células da bactéria. Foi utilizado o método do número mais provável (NMP nos ágares para isolamento verde brilhante com novobiocina (BGN e xilose-lisina tergitol 4 (XLT4. Os resultados mostraram 12,2% de ocorrência de Salmonella nas carcaças de frangos resfriadas e a média de NMP de Salmonella por mL, na leitura pelo ágar XLT4 foi de 2,7 células e no ágar BGN foi de 1,3 células. Os sorovares de Salmonella isolados das carcaças de frangos no estudo foram S. Enteritidis, S. Agona, S.Rissen, S. Heidelberg e S. Livingstone. A análise dos resultados demonstrou existir um número variável de células de Salmonella contaminando as carcaças de frango resfriadas que estão à venda ao consumidor.Salmonella in poultry remains an important worldwide problem, and among foodborne pathogens, the Salmonella appears as one of the most important outbreaks agents. To assess the risks of acquiring infection via undercooked poultry or cross contamination from chickens, it is important to determine the extent of the contamination on raw poultry with this pathogen. In this study, 180 refrigerated broiler carcasses, obtained from local stores, were assessed to recover Salmonella by the most probable number (MPN method to quantify bacterias cells onto brilliant green agar with novobiocin (BGN and xylose lysin tergitol 4 agar

  1. Transition from diffusive to localized regimes in surface corrugated waveguides

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    Garcia-Martin, A.; Saenz, J. J. [Universidad Autonoma de Madrid, Madrid (Spain); Nieto-Vesperinas, M. [Instituto de Ciencias de Materiales de Madrid, Madrid (Spain)

    2001-03-01

    rugosa de la guia de onda crece, se observa una transicion desde el regimen de difusion al de localizacion. Esto establece una analogia con la conduccion de electrones en nanocables, y asi podemos introducir un concepto analogo al de la resistencia electrica. Predecimos un comportamiento oscilatorio de diferentes magnitudes caracteristicas del transporte (recorrido libre medio elastico, longitud de localizacion, enhanced bakckscattering) en funcion de la longitud de onda. El analisis de los coeficientes de transmision (speckle transmitido) muestra que, a medida que la longitud de la zona rugosa aumenta, hay una marcada tendencia al acoplamiento con el modo mas bajo. Esto demuestra la existencia de una clara anisotropia en la propagacion de las ondas, la cual esta ausente en el caso de guias con desorden de volumen. La estadistica de los coeficientes de reflexion es analizada usando Teoria de Matrices Aleatorias (RMT) para deducir analiticamente las densidades de probabilidad en el regimen de localizacion, las cuales seran posteriormente comparadas con calculos numericos exactos del acoplamiento de modos en reflexion para el caso de guias de onda con desorden de superficie. Mostrando que las distribuciones en reflexion son independientes del regimen de transporte, en contraposicion con la transicion de regimen observada para el caso de la transmision. A pesar de la marcada anisotropia, el analisis de las distribuciones de probabilidad tanto en reflexion como en transmision confirman las predicciones obtenidas por medio de RMT para desorden de volumen.

  2. Estrés alimentario y salud laboral vs. estrés laboral y alimentación equilibrada

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    Juan José Díaz Franco

    2007-12-01

    Full Text Available Si bien el estrés laboral es una realidad experimentada cada jornada por muchos trabajadores, debemos reconocer que algunas profesiones y actividades laborales generan más estrés (distrés que otras: así, los trabajos de dirección, nocturnos, monótonos, de servicios comunitarios, de industria pesada y minería, de construcción, etc. La mayor acumulación de estrés laboral puede alterar los patrones de conducta de quienes lo sufren. De manera muy especial, los hábitos alimentarios y las conductas asociadas a ellos pueden verse seriamente afectados por el estrés laboral (ingestas inadecuadas o extemporáneas, o pueden, "sensu contrario", ser desencadenantes de formas de estrés originadas por alteraciones o desórdenes dietéticos. Por eso no extraña que el aparato digestivo se convierta en órgano diana de patologías asociadas al estrés propio de ambientes y ritmos laborales insanos y vitandos. El estrés laboral puede alterar el apetito y el hambre y, como consecuencia, la correcta conducta alimentaria y la nutrición que, inexorable e involuntariamente, sigue a ésta. En determinadas circunstancias, hay disociación entre el apetito (sensación primariamente psíquica y el hambre (sensación básicamente orgánica. Y es que el apetito, como estado mental, puede verse modificado por diferentes situaciones emocionales (muy frecuentemente, por el estrés laboral, o a través de variaciones químicas y vasculares y de impulsos transmitidos al mesencéfalo desde diversos órganos, donde el estrés podría desempeñar también un papel relevante. La nutrición depende esencialmente de la alimentación; pero, a su vez, una fuente importante de estrés la constituye la forma en que nos alimentamos, porque si lo hacemos erróneamente podemos contraer enfermedades. Si nuestras células no reciben los aportes nutritivos adecuados, se produce una gran carga de estrés debida a esta demanda interna insatisfecha.Although occupational stress is a

  3. Photosynthetic pigments content, photosynthesis rate and chloroplast structure in young plants of Mikania laevigata Schultz Bip. ex Baker grown under colored netsTeores de pigmentos fotossintéticos, taxa de fotossíntese e estrutura de cloroplastos de plantas jovens de Mikania laevigata Schultz Bip. ex Baker cultivadas sob malhas coloridas

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    Anacleto Ranulfo dos Santos

    2011-12-01

    menores teores de clorofila a e b e o menor teor de carotenóides, enquanto aquelas cultivadas sob malha azul tiveram a maior concentração de clorofilas a e b. A menor densidade de cloroplastos foi encontrada nas plantas cultivadas a pleno sol. Nos tratamentos com 50% de sombreamento, foram verificados cloroplastos mais alongados. Com relação à taxa de fotossíntese potencial, não foi observada nenhuma diferença significativa entre plantas crescidas sob as malhas vermelha, cinza e azul, fato que leva a acreditar que o espectro diferenciado transmitido pelas malhas coloridas não interferiu no aparelho fotossintético do guaco.

  4. Proposta de uma metodologia para o ensino da estrutura e função das proteínas na disciplina Bioquímica

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    George Sabino

    2009-02-01

    Full Text Available A Bioquímica é uma disciplina fundamental para uma formação superior crítica e reflexiva do estudante da área biológica e da saúde. A Bioquímica é uma ciência ampla, a qual lida com assuntos complexos e abstratos, o que muitas vezes dificulta seu entendimento pelos alunos e acaba contribuindo para um alto percentual de reprovação na disciplina. O objetivo do presente trabalho foi desenvolver uma metodologia de ensino/aprendizagem para minimizar as dificuldades de entendimento do conteúdo didático, explorar as potencialidades de aplicação desse conteúdo e facilitar o estudo da estrutura e função das proteínas na disciplina Bioquímica. METODOLOGIA: Com o intuito de introduzir e contextualizar conceitos bioquímicos a respeito da estrutura e função das proteínas, cinco estratégias foram propostas: confecção de modelos de biomoléculas com materiais de baixo custo; uso do corpo como ferramenta pedagógica para a fixação de conceitos básicos; elaboração de um roteiro de procedimentos de laboratório com substâncias reagentes do dia a dia para demonstração prática do conteúdo teórico; contextualização do tema em relação ao cotidiano do aluno e sua futura prática profissional; e uso da internet de forma complementar ao processo de ensino/aprendizagem iniciado em sala de aula. A metodologia proposta foi realizada em uma turma de 35 alunos do 2o período de graduação de enfermagem e os resultados foram avaliados através da percepção do professor e do preenchimento, pelos alunos, de um questionário elaborado especificamente para este estudo. RESULTADOS E DISCUSSÃO: Foram gastos 150 minutos para a realização das estratégias elaboradas na metodologia. Na opinião do professor, o conteúdo pôde ser transmitido de uma forma fácil e os alunos se mostraram abertos e interessados com a exposição. Dos dezoito alunos que responderam ao questionário, todos foram unânimes em elogiar os modelos elaborados

  5. Morte súbita dos citros: suscetibilidade de seleções de limão-cravo e uso de interenxertos Citrus sudden death: susceptibility of rangpur lime selections and the use of interstocks

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    Jorgino Pompeu Junior

    2008-12-01

    Full Text Available A morte súbita dos citros é uma doença que afeta cultivares de laranjas e tangerinas enxertadas nos limões-Cravo e Volkameriano. Ela foi observada em plantas com dois a seis anos de idade que, após mostrarem sintomas gerais de declínio, entraram em colapso e morreram. A retirada da casca dos porta-enxertos suscetíveis revela o amarelecimento na região cambial, sendo esse o sintoma-diagnóstico da doença e que precede os sintomas da copa. As plantas enxertadas nas tangerinas Cleópatra e Sunki, no trifoliata e no citrumelo Swingle, não mostram sintomas da doença. A transmissão por borbulha e a evolução espacial sugerem que a morte súbita dos citros seja causada por patógeno transmitido por vetor alado. Com o objetivo de selecionar porta-enxertos tolerantes à doença, laranjeiras Valência enxertadas em 254 porta-enxertos foram plantadas em maio de 2003 e 2004 em solos onde foram erradicados pomares afetados pela morte súbita dos citros e próximos a pomares afetados pela doença. Em novembro de 2006, o sintoma-diagnóstico da doença estava presente em dez seleções de limão-Cravo: Santa Barbara red lime, Borneo red lime, Limão-Cravo Taquaritinga, Rangpur India C-26-1, Rangpur rose lemon, Rangpur Kusaie lime, Rangpur red lime D-33-40, Rangpur Egyptian lime, Rangpur lemon India e Japanshe citroen. A presença de interenxerto de trifoliata ou de tangerina Cleópatra, entre o limão-Cravo e a laranja Valência, não impediu a manifestação da doença.Citrus sudden death (CSD or morte súbita dos citros affects sweet orange cultivars and some mandarin trees grafted on Rangpur lime and Volkamer lemon rootstocks. The disease was observed in trees with ages ranging from two to six years; after showing general decline symptoms, the affected trees suddenly collapse and die. Trees on Cleopatra and Sunki mandarins, 'Swingle' citrumelo and trifoliate orange showed no symptoms of CSD. Cambial yellowing in the rootstock can be observed

  6. Plantas medicinais usadas para a saúde bucal pela comunidade do bairro Santa Cruz, Chapada dos Guimarães, MT, Brasil Medicinal plants used for oral health in the Santa Cruz neighborhood, Chapada dos Guimarães, Mato Grosso State, Brazil

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    Aneliza Meireles Borba

    2006-12-01

    Full Text Available Em Mato Grosso, populações tradicionais recorrem ao uso de espécies vegetais como alternativa terapêutica. Na cidade de Chapada dos Guimarães, o bairro Santa Cruz se destaca por abrigar famílias nascidas em áreas urbanas ou rurais que conservam esses conhecimentos transmitidos por gerações. Esta pesquisa tem como objetivo o levantamento das plantas medicinais utilizadas pela comunidade local, indicações terapêuticas, preparos e modos de uso visando a manutenção e recuperação da saúde bucal. Foram entrevistados 40 residentes, através de abordagem qualitativa, usando entrevista semi-estruturada. As espécies catalogadas foram depositadas para identificação no UFMT/Herbário Central. Foram citadas 87 espécies pertencentes a 48 famílias utilizadas na saúde bucal, encontradas no bioma Cerrado ou cultivadas nas residências. Conforme as afecções bucais citadas, as espécies utilizadas são: para erupção dentária: camomila (Matricaria chamomilla L.; candidíases, estomatites, gengivites e afta: açafrão (Crocus sativus L.; dor de dente: arnica-da-serra (Brickelia brasiliensis (Spreng. Robinson. A folha foi a parte da planta mais usada e o chá, por decocção, modo de preparo mais comum. Pessoas idosas, líderes comunitários, parteiras e benzedeiras entrevistados apresentaram um maior conhecimento sobre o assunto. Constatou-se que a comunidade utiliza espécies vegetais, nativas do cerrado ou exóticas, com finalidade terapêutica para manutenção e recuperação da saúde bucal, sendo uma alternativa tradicional, econômica e atuante.In the State of Mato Grosso, traditional populations turn to the use of many plant species as a therapeutic alternative. In the city of Chapada dos Guimarães, the Santa Cruz neighborhood is well known for harboring families from urban and rural communities who preserve this knowledge passed down through many generations. The objective of this research was to survey the medicinal plants used

  7. Detection of PMTV Using Polyclonal Antibodies Raised Against a Capsid-Specific Peptide Antigen / Detección de PMTV Utilizando Anticuerpos Policlonales Contra un Péptido Antigénico Derivado de la Cápside Viral

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    Yuliana Gallo García

    2013-12-01

    Full Text Available Potato mop-top virus (PMTV; genus Pomovirus;family Virgaviridae is the causing agent of the spraing disease in potato (Solanum tuberosum. PMTV is transmitted by Spongospora subterranea f. sp. subterranea (Sss. This disease has a widespread distribution in potato growing regions around the world. The possibility of obtaining strain specific antibodies at low cost can greatly increase the sensitivity and use of serological tests in seed certification programs, plant breeding and quarantine regulations to avoid dissemination of this injurious virus. This work presents an alternative procedure for the production of PMTV specific antibodies useful in serological test such as ELISAand lateral flow. In contrast to standard methods requiring theisolation of viral particles or expression of recombinant capsid, this method uses peptides mimicking the N-terminal region of PMTV capsid protein as antigen for the production of specific polyclonal antibodies. The antibodies were tested against bait plants grown in soil infested with viruliferous Sss, as well as potato plants obtained from naturally Sss infested fields in Colombia. PMTV was detected in 9/14 and 24/28 foliage samples of N. benthamiana and S. phureja, respectively. In the case of field plants, the virus wasdetected in eight out of 12 root tissues evaluated. The minimumpeptide concentration detected by ELISA was of the order of 0.1 nM. / Potato mop-top virus (PMTV; género Pomovirus; familia Virgaviridae es transmitido por Spongospora subterranea f. sp. subterranea (Sss, agente causal de la sarna polvosa de la papa. Esta enfermedad tiene una amplia distribución en las regiones cultivadoras de papa alrededor del mundo. La posibilidad de obtener anticuerpos específicos contra cepas de este virus, puede incrementar la sensibilidad y la utilización de pruebas serológicas en programas de certificación de semilla, mejoramiento genético y regulaciones cuarentenarias que eviten su diseminaci

  8. Guinea pigs experimentally infected with vaccinia virus replicate and shed, but do not transmit the virus Cobaias infectadas experimentalmente com vírus vaccínia replicam e excretam, porém não transmitem o vírus

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    Juliana Felipetto Cargnelutti

    2012-06-01

    secreções nasais (1 a 9 dias pós-inoculação - pi, desenvolveram viremia (1 a 10 dias pi e soroconverteram ao VACV. Apesar da replicação e excreção viral, o vírus não foi transmitido a sentinelas por contato direto, indireto (aerossóis ou por água e alimentos contaminados com fezes deliberadamente contaminadas com o vírus. Esses resultados demonstram que, apesar de replicar e excretar o vírus, as cobaias não transmitem o VACV nas condições estudadas. Esses achados tornam pouco provável a participação de espécies relacionadas na manutenção e transmissão do VACV na natureza.

  9. Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp Caracterização molecular adicional de begomovírus associados a plantas daninhas no Brasil, com ênfase em Sida spp

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    S.S. Tavares

    2012-06-01

    Full Text Available Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants. The aim of this study was to further characterize the diversity of begomoviruses infecting weeds (mostly Sida spp. in Brazil. Total DNA was extracted from weed samples collected in Viçosa (Minas Gerais state and in some municipalities of Alagoas state in 2009 and 2010. Viral genomes were amplified by RCA, cloned and sequenced. A total of 26 DNA-A clones were obtained. Sequence analysis indicated the presence of 10 begomoviruses. All viral isolates from Blainvillea rhomboidea belonged to the same species, Blainvillea yellow spot virus (BlYSV , thereby suggesting that BlYSV may be the only begomovirus present in this weed species. Four isolates represent new species, for which the following names are proposed: Sida yellow blotch virus (SiYBV, Sida yellow net virus (SiYNV, Sida mottle Alagoas virus (SiMoAV and Sida yellow mosaic Alagoas virus (SiYMAV. Recombination events were detected among the SiYBV isolates and in the SiYNV isolate. These results constitute further evidence of the high species diversity of begomoviruses in Sida spp. However, the role of this weed species as a source of begomoviruses infecting crop plants remains to be determined.Begomovírus são vírus de DNA circular fita simples transmitidos por mosca branca, os quais são frequentemente associados com plantas daninhas. O objetivo deste trabalho foi caracterizar a diversidade de begomovírus infectando plantas daninhas (principalmente Sida spp. no Brasil. DNA total foi extraído a partir de plantas daninhas coletadas em Viçosa (Minas Gerais e em alguns municípios do estado de Alagoas em 2009 e 2010. Os genomas virais foram amplificados por RCA, clonados e sequenciados. Um total de 26 clones de DNA-A foram obtidos. A análise das sequências indicou a presença de dez diferentes begomovírus. Todos os isolados originários de Blainvillea rhomboidea pertencem a uma

  10. Parent-to-offspring transfer of sublethal effects of copper exposure: Metabolic rate and life-history traits of Daphnia Transferencia paterno-filial de efectos subletales de la exposición al cobre: Tasa metabólica y rasgos de historia de vida en Daphnia

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    MAURICIO A FERNÁNDEZ-GONZÁLEZ

    2011-06-01

    Full Text Available In ecological communities, pollution driven perturbations exert immediate effects on sensitive individuals, but these effects may be transmitted among interacting organisms and spread over the community through several paths. This makes the assessment and prediction of ecological consequences of pollution difficult. The propagation of perturbation effects among organisms can be horizontal among organisms that coexist in space and time, and vertical among organism that belong to different generations. The latter process is poorly understood, in particular in planktonic organisms facing metal pollution. In this study we evaluate the vertical transfer of effects driven by sublethal copper stress on the heartbeat rate, somatic growth and fertility of Daphnia pulex. In order to evaluate this, we performed a factorial experiment in which parental and filial generations were exposed to both copper-enriched and control media. We found that parental exposure to copper exerted a significant effect on the heartbeat rate, somatic growth and fertility of offspring, revealing a transgenerational effect in D. pulex. This response may be explained by a higher resource investment on repair/detoxification processes in the parental generation, allocating fewer resources to offspring quality. Our results suggest that responsiveness of organisms to stress is dependent on parental history.En las comunidades ecológicas, las perturbaciones producidas por los contaminantes ejercen efectos inmediatos en los individuos sensibles, pero estos efectos podrían ser transmitidos entre los organismos interactuantes y extenderse sobre la comunidad a través de múltiples vías. Esto hace difícil la evaluación y predicción de las consecuencias ecológicas de la contaminación. La propagación de los efectos de una perturbación entre los organismos puede ser horizontal, entre organismos que coexisten espacial y temporalmente, y vertical, entre organismos que pertenecen a

  11. Garaje para la Universidad de California

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    Lin, T. Y.

    1964-06-01

    presencia de grietas. Esta disposición en el trazado de los cables del pretensado asegura la impermeabilización del hormigón e introduce ventajas económicas, ya que hace innecesaria toda capa de impermeabilización. Como el edificio se halla rodeado en tres de sus fachadas por otros tantos muros de contención, se ha sacado partido de esta circunstancia para arriostrar los forjados sobre estos muros y transmitir sobre ellos los efectos del viento y sísmicos, en una dirección, mientras que, en la normal a ella, estos efectos son transmitidos al terreno por el intermedio de los soportes. La mayoría de los elementos estructurales, a excepción de las losas para forjados, han sido prefabricados y pretensados en un taller situado a unos 80 km de distancia de la obra. Muchos de los elementos cuentan con armaduras complementarias para el postensado después de haber sido colocados en obra.

  12. Estratégias de acesso ao Ensino Superior entre jovens universitários com experiência de trabalho na infância/ Strategies of access to the higher education among young college students with work experience during the childhood

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    Andrea Abreu Astigarraga

    2010-09-01

    Full Text Available Este artigo aborda o modus operandi de jovens egressos do Ensino Superior, oriundos da zona rural, com experiência de trabalho na infância e que acessaram os cursos elitizados da Universidade Estadual Vale do Acaraú, Sobral, Ceará. A pesquisa autobiográfica dialogada foi relevante, tendo em vista que, em suas histórias de vida, as estratégias pessoais e familiares preponderaram diante do contexto de pobreza, subsistência, desde a infância, no Ensino Fundamental, passando pela adolescência, no Ensino Médio, à juventude, na Universidade. As principais estratégias verificadas em suas narrativas estão relacionadas com o capital social (relações interpessoais, os saberes e os valores transmitidos pela família. Verifica-se a urgência de políticas públicas de inclusão no Ensino Superior, para atender a uma demanda considerável de pessoas que não têm acesso a ela ou a acessam com muitas dificuldades, principalmente na UVA que, por ser universidade de âmbito estadual, não aplica as novas políticas “democráticas” do Ensino Superior, tais como ENEM e PROUNI. Os resultados da pesquisa poderão ser aplicados tanto no âmbito micro quanto no macro. O micro espaço da sala de aula pode sofrer grande influência no processo ensino-aprendizagem, quando se conhecem as estratégias escolares utilizadas pelos alunos. A administração central da universidade, visando ao planejamento estratégico de instâncias administrativas, tais como Pró-Reitoria de Graduação, Assuntos Estudantis, Iniciação Científica e Pesquisa, também poderá beneficiar-se deste estudo para a avaliação institucional. This article deals with the modus operandi of the egressed students of higher education, native from the rural area, with work experience in their childhood and that had access to the best courses at Vale do Acaraú University, Sobral, Ceará. The discussed autobiographical research was relevant bearing in mind that, in their life history

  13. A influência do ambiente familiar e escolar na aquisição e no desenvolvimento da linguagem: revisão de literatura Family and school environment influences on language acquisition and development: literature review

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    Ramilla Recla Scopel

    2012-08-01

    Full Text Available TEMA: o termo linguagem é definido como organizado sistema de símbolos, com propriedades particulares que desempenham a função de codificação, estruturação e consolidação dos dados sensoriais, o que permite que experiências sejam comunicadas e seus conteúdos transmitidos. O desenvolvimento da linguagem depende não somente das condições biológicas inatas de cada indivíduo, como também sofre influência de fatores ambientais presentes nos meios em que as crianças estão inseridas, como por exemplo, a família e a escola. OBJETIVO: descrever e analisar as produções científicas relevantes para o entendimento da influência do ambiente familiar e escolar na aquisição e desenvolvimento da linguagem em crianças por meio de revisão da literatura. CONCLUSÃO: as produções científicas apresentadas apontam a relevância da estimulação no âmbito familiar e escolaridade dos pais, porém pôde-se perceber a carência de estudos que relacionassem o desenvolvimento infantil com o ambiente escolar. Outro fator que chamou atenção foi a falta de estudos que correlacionem vocabulário e fonologia com os fatores ambientais escolares e familiares. A partir da revisão da literatura abrem-se novas perspectivas de trabalhos a serem realizados na Fonoaudiologia para que as lacunas existentes possam ser preenchidas com novos conhecimentos sobre desenvolvimento infantil.BACKGROUND: the term language is defined as organized system of symbols, with properties, that has a role in encoding, consolidation and structuring sensory data, allowing for experiments to be reported and their contents communicated. Language development depends not just on the innate biological conditions of each individual, but is also influenced by environmental factors in the environments where children are inserted, such as family and school. PURPOSE: to describe and analyze scientific studies relevant to understanding the influence of family and school environment

  14. Variabilidade genética entre isolados de Colletotrichum gossypii do algodoeiro Genetic variability among the isolates of Colletotrichum gossypii of cotton

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    Yeshwant R. Mehta

    2010-03-01

    Full Text Available O algodoeiro é atacado por Colletotrichum gossypii (CG e C. gossypii var. cephalosporioides (CGC. Ambos os patógenos são transmitidos pela semente e sua distinção morfológica é extremamente difícil e inconsistente. Tentativas foram feitas no presente trabalho para verificar a variabilidade genética entre CG e CGC através de RAPD-PCR, ERIC- e REP-PCR e PCR-RFLP da região ITS rDNA. Foram utilizados 53 isolados coletados de sementes e folhas de plantas de diferentes cultivares nos estados do Paraná, São Paulo, Mato Grosso, Minas Gerais, e Paraiba, entre 1999 e 2003. Baseado em testes de patogenicidade, vinte e um isolados foram classificados como CG e 32 como CGC. Os resultados obtidos por RAPD-PCR, utilizando-se oito primers, revelaram dois grupos distintos sendo que o primeiro foi formado por 94% dos isolados de sementes e o segundo por 95% dos isolados de folhas. Na análise de ERIC- e REP-PCR, resultados semelhantes a RAPD foram obtidos, sendo que o primeiro grupo foi formado por 93% dos isolados provenientes das sementes e o segundo por 78% dos isolados provenientes das folhas. Quando o produto de amplificação da região ITS rDNA foi digerido com oito enzimas de restrição, um perfil de bandas semelhante para todos os isolados foi obtido. Resultados de RAPD, ERIC- e REP-PCR demonstraram que existem diferenças genéticas entre os isolados provenientes das sementes e aqueles provenientes de parte aérea, e esses dois grupos foram claramente distintos. Estudos futuros devem ser realizados utilizando outras técnicas moleculares para a obtenção de marcadores capazes de distinguir entre isolados de CG e CGC.Cotton is attacked by Colletotrichum gossypii (CG and C. gossypii var. cephalosporioides (CGC. Both the pathogens are transmitted by seed and their morphological distinction is extremely difficult and inconsistent. In the present study, attempts were made to verify the genetic variability among 53 isolates of CG and CGC using

  15. Implicaciones en la salud humana de la gastroenteritis aguda por norovirus como producto de la contaminación hídrica

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    Brenda Delgadillo Espinoza

    2011-05-01

    Full Text Available En julio del 2010, un brote de gastroenteritis Aguda surgió entre los residentes de la ciudad principal de la Región Norte de Costa Rica: Ciudad Quesada. Esta investigación se llevó a cabo para explorar y caracterizar el brote en esa zona rural. Los datos de vigilancia y los registros médicos de los pacientes fueron analizados para elaborar un estudio descriptivo entre el 08 de julio y 14 de julio del 2010 en el Cantón de San Carlos. Una hoja de datos (Ficha Colectiva de Investigación VE 0.2 fue aplicada para obtener información de los pacientes. Con el fin de detectar la presencia de enteropatógenos, una prueba de ELISA fue aplicada a las muestras de heces tomadas. Las muestras de agua también se analizaron para detectar bacterias y coliformes fecales en el sistema de distribución. Un total de 352 casos fueron confirmados en la región estudiada con la presencia de síntomas frecuentes entre los casos confirmados; estos síntomas fueron diarrea (94,2%, náuseas (92,7% y vómitos (91,7%. La edad media de los pacientes fue de 29 años (15,8 y el 50,8% correspondió a sujetos del sexo femenino. El grupo de edad más afectado es el representado por los lactantes de 1 a 4 años (tasa de ataque 18,2 / 1000 habitantes La zona con más afectación fue el área central de Ciudad Quesada. En cinco de cada seis pruebas de heces fue posible aislar el norovirus. Por otra parte, en las pruebas aplicadas al agua solo se identifica la presencia de Escherichia coli. Los resultados sugieren que el norovirus fue transmitido por agua contaminada con materia fecal. Este es el primer brote documentado que ha sido causado por este virus en la región norte del país. Se recomienda mejorar la vigilancia de la calidad del agua, promover el uso de agua potable entre los residentes, generar un plan de educación que fomente prácticas seguras de manejo y distribución de agua; así como campañas de sensibilización en comunidades rurales como ésta en temas

  16. Diversidade inter- e intra-específica e uso de análise multivariada para morfologia da mandioca (Manihot esculenta Crantz: um estudo de caso Inter- and intraspecific diversity and use of multivariate analysis for the morphology of cassava (Manihot esculenta Crantz: a case study

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    Nivaldo Peroni

    1999-07-01

    Full Text Available A agricultura itinerante, caracterizada por ciclos de uso e pousio, é uma técnica utilizada por muitos agricultores no mundo, assim como por agricultores tradicionais da região de Cananéia (SP. Estes agricultores herdaram toda um gama de conhecimentos transmitidos oralmente sobre a agricultura praticada por povos indígenas desde o período pré colonial. A mandioca é o principal cultivo neste sistema, o que a torna um organismo chave para entender as especificidades do sistema agrícola itinerante. Foram obtidas informações sobre as espécies cultivadas no sistema itinerante, e mais especificamente sobre as etnovariedades de mandioca. Entrevistas com os agricultores permitiram caracterizar o sistema utilizado, demonstrando que na propriedade estudada podem ser cultivadas até 62 etnovariedades pertencentes a 15 espécies diferentes, mostrando a grande diversidade tanto inter como intra específica. O objetivo principal foi aferir a identificação feita pelo agricultor das etnovariedades de mandioca (Manihot esculenta Crantz cultivadas intra-roça e a divergência morfológica entre elas. Através da avaliação de 21 caracteres morfológicos avaliados in situ, em uma roça de agricultores tradicionais da comunidade de Ariri (Cananéia, e utilizando análise de componentes principais (PCA e análise de agrupamento, foi possível agrupar as etnovariedades em grupos coerentes com a classificação local. Além disso a metodologia empregada mostrou grande eficiência com dados tomados em condições não experimentais.Shifting cultivation, or slash-and-burn agriculture, is a technique used by many farmers all over the world and also by traditional farmers of Cananéia (SP. These farmers have inherited a set of skills and knowledges, orally transferred through generations, about the agriculture carried out by indigenous people, since Brazil's pre-colonialist period. Cassava (Manihot esculenta Crantz is the main crop in this system

  17. Método do rolo de papel toalha modificado para a detecção de Sclerotinia sclerotiorum em sementes de feijão Modification of the paper towel seed health test for the detection of Sclerotinia sclerotiorum in bean seeds (Phaseolus vulgaris L.

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    João José Dias Parisi

    2006-09-01

    Full Text Available O mofo branco do feijoeiro, causado pelo fungo Sclerotinia sclerotiorum (Lib. de Bary, é uma das principais doenças da cultura. O patógeno pode ser disseminado por sementes infectadas, que têm um importante papel na infestação de novas áreas de plantio e no estabelecimento da doença no início do ciclo da cultura. Este trabalho apresenta uma adaptação do método do rolo de papel toalha, originalmente desenvolvido para a detecção de Colletotrichum lindemuthianum, com o objetivo de detectar a presença de S. sclerotiorum em sementes de feijão. Neste método, as sementes foram incubadas por 7 dias a 20 ºC em rolos de papel toalha para germinação, sendo mantidas sob condições de 100% de umidade relativa. Após esse período, as plântulas infeccionadas e as sementes mortas, circundadas por micélio característico de S. sclerotiorum, foram transportadas para caixas tipo gerbox, sobre duas folhas de papel de filtro umedecido. Após 3 a 4 dias de incubação a 20 ºC e sob regime de 12 horas de luz por 12 horas de escuro, os escleródios foram observados nas sementes e plântulas. O método foi relativamente rápido, simples e barato, além de apresentar a vantagem de possibilitar a detecção simultânea de S. sclerotiorum e de outros importantes patógenos transmitidos por sementes de feijão, como C. lindemuthianum, Macrophomina phaseolina e Rhizoctonia solani.White mold, caused by the fungus Sclerotinia sclerotiorum (Lib. is one of the most damaging diseases of beans. Dissemination of the pathogen by infected seeds is important because it can infest new planting areas and the disease may establish in the beginning of the crop cycle. The present work describes an adaptation of the seed health test using the germination paper towel method, originally developed for the detection of Colletotrichum lindemuthianum, for assessing the presence of S. sclerotiorum in bean seeds. The test consisted of placing bean seeds in germination paper

  18. Aislamiento de Candida spp. y otras levaduras en el personal que labora en áreas críticas del Hospital San Juan de Dios Isolation of Candida spp. and other yeasts from Health Care Workers in Critical Care Units of the San Juan de Dios Hospital

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    Pedro Carrillo-Dover

    2009-09-01

    Full Text Available Justificación y objetivos: Los pacientes internados en hospitales, principalmente aquellos que se encuentran severamente enfermos, son más susceptibles a las infecciones por hongos oportunistas, en comparación con la población general. El personal hospitalario puede ser fuente potencial de infección para estos pacientes, ya que normalmente actúa como portador de gérmenes, que eventualmente podrían ser transmitidos a los pacientes. Se describe, en esta investigación, el aislamiento de hongos levaduriformes a partir de las manos y la cavidad oral, en un grupo de 77 trabajadores del Hospital San Juan de Dios, en servicios donde se han reportado más casos de infecciones por este tipo de hongos. Métodos: Se realizó un hisopado de cavidad oral y manos de cada participante y se cultivaron placas con agar glucosado de Sabouraud (AGS y Mycosel. A los aislamientos de levaduras se les determinó la capacidad de crecimiento a 37°C, resistencia a la cicloheximida, producción de tubo germinativo, fosfolipasas y proteinasas y se determinó la sensibilidad in vitro al fluconazol por medio de método de microdilución en placa. Resultados: El 72,7% de los participantes resultaron positivos para el aislamiento de levaduras, la especie aislada con mayor frecuencia fue Candida parapsilosis, seguida de C. albicans y C. famata. La mayor positividad se obtuvo en el servicio de Cirugía 3 (83.3%, seguido de la UCI (71.4% y Neonatología (58%. Conclusión: Estos resultados instan a mejorar las acciones preventivas en el manejo de los pacientes, a ser más estrictos en las normas de higiene de manos y promover, en otros centros hospitalarios, la realización de este tipo de estudios, para disminuir los brotes nosocomiales por transmisión horizontal.Justification and aim: Critically ill patients are more susceptible than the general population to opportunistic fungal infections. Health workers could be a potential infectious focus to these patients. Thus in

  19. O ato de comer e as pessoas com Síndrome de Down El acto de comer y las personas con Sindrome de Down Eating act and people with Down Syndrome

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    Andréa Giaretta

    2009-06-01

    Full Text Available Trata-se do relato de experiência vivida durante a disciplina de Projetos Assistenciais de Enfermagem e de Saúde do Curso de Mestrado em Enfermagem da Universidade Federal de Santa Catarina de cunho interdisciplinar, realizado com pessoas com Síndrome de Down com foco tanto na sua racionalidade quanto no seu imaginário. Buscou-se construir junto com estas pessoas e seus familiares uma sistematização nutricional individualizada que contribuísse para sua independência pessoal na prática cotidiana do ato de comer. Os postulados do Interacionismo Simbólico guiaram esta prática e se interligaram com os instrumentos metodológicos da escuta e da observação sensível, além das atividades lúdicas. Evidenciou-se que o primeiro significado do ato de comer é construído pela sua família e reforçado pelo seu convívio social.Eso es un relato de una experiencia vivida en la disciplina de Proyectos Asistenciales de Enfermaría y de Salud del Curso de Maestría en Enfermaría de la Universidad Federal de Santa Catarina con característica interdisciplinario, hecho con personas con el Síndrome de Down, con la atención tanto para la racionalidad cuanto para el imaginario. Se ha buscado construir juntamente a las personas con el Síndrome de Down y sus familiares una sistematización de nutrición individualizada que contribuyese para su independencia personal en la práctica cotidiana del acto de comer. Los postulados del Interaccionismo Simbólico condujeron la práctica y interconectáronse con los instrumentos metodológicos de la escucha y de la observación sensible, además de las actividades lúdicas. Fue evidenciado que el primer significado del acto de comer es transmitido por sus familias y reforzado por el convivir social.This is en experience report lived in the Health and Nursing Assistance Projects of the Post-Graduate Nursing Master Degree of the Federal University of Santa Catarina as an interdisciplinary subject, and it was

  20. Análise da distribuição espaço-temporal de dengue e da infestação larvária no município de Tupã, Estado de São Paulo Analysis on the spatial-temporal distribution of dengue and larval infestation in the municipality of Tupã, State of São Paulo

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    Gerson Laurindo Barbosa

    2010-04-01

    Full Text Available INTRODUÇÃO: O vírus da dengue é transmitido pela picada do mosquito Aedes aegypti e, o atual programa de controle não atinge o objetivo de impedir sua transmissão. Este trabalho objetivou analisar a relação entre a distribuição espaço-temporal de casos de dengue e os indicadores larvários no município de Tupã, de janeiro de 2004 a dezembro de 2007. MÉTODOS: Foram construídos indicadores larvários por quarteirão e totalidade do município. Utilizou-se o método cross-lagged correlation para avaliar a correlação entre casos de dengue e indicadores larvários. Foi utilizado estimador kernel para análise espacial. RESULTADOS: A correlação cruzada defasada entre casos de dengue e indicadores larvários foi significativa. Os mapas do estimador Kernel da positividade de recipientes indicam uma distribuição heterogênea, ao longo do período estudado. Nos dois anos de transmissão, a epidemia ocorreu em diferentes regiões. CONCLUSÕES: Não ficou evidenciada relação espacial entre infestação larvária e ocorrência de dengue. A incorporação de técnicas de geoprocessamento e análise espacial no programa, desde que utilizados imediatamente após a realização das atividades, podem contribuir com as ações de controle, indicando os aglomerados espaciais de maior incidência.INTRODUCTION: The dengue virus is transmitted by the bite of the mosquito Aedes aegypti and the current control program has not met the target of preventing its transmission. This study aimed to analyze the relationship between the spatial-temporal distribution of dengue cases and the larval indices in the municipality of Tupã between January 2004 and December 2007. METHODS: Larval indices were built up block by block and for the entire municipality. We used the cross-lagged correlation method to assess the correlation between dengue cases and larval indices. The kernel estimator was used for spatial analysis. RESULTS: The cross-lagged correlation

  1. Seguimento dos doentes soropositivos e soronegativos para o HIV com carcinoma espinocelular do canal anal Follow-up for HIV negative and HIV-positive patients with anal squamous cells carcinoma

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    Sidney Roberto Nadal

    2009-09-01

    Full Text Available A incidência do carcinoma espinocelular (CEC anal e das neoplasias intra-epiteliais anais (NIA é maior nos pacientes infectados pelo papilomavírus humano (HPV, e está relacionada à imunidade e à infecção pelo vírus da imunodeficiência humana (HIV. A associação com viroses de transmissão sexual indica que seja tumor sexualmente transmitido. A infecção pelo HIV mudou o perfil dos portadores do CEC anal. A doença que anteriormente acometia mulheres na 6ª década de vida, hoje atinge preferencialmente homens na 3ª e 4ª décadas. Nos Estados Unidos, a expectativa de diagnóstico desse tumor aumentou de 19/100.000, na época pré-HAART (1992-1995, para 48,3/100.000 no período pós-HAART imediato (1996-1999 e para 78,2/100.000 pessoas por ano, nos anos mais recentes (2000-2003. O tratamento do CEC anal, descrito por Nigro em 1974, combina radio e quimioterapia. Operações de resgate estão indicadas caso haja persistência ou recidiva da doença. Estudos comparando, respectivamente, doentes imunodeprimidos e imunocompetentes vêm mostrado envolvimento linfonodal em 60% e 17%, recidivas em 75% e 6%, boa resposta à radio e quimioterapia em 62% e 85%, toxicidade a esse tratamento em 80% e 30%, e sobrevivência global de 1,4 e 5,3 anos. A contagem sérica baixa de linfócitos T CD4 prediz mau prognóstico. Quando acima de 200/mm³, os resultados são comparáveis aos observados entre os imunocompetentes.Anal squamous cell carcinoma (ASCC and anal intra-epithelial neoplasia (AIN incidences are bigger among human papillomavirus (HPV infections, and are associated to immunity and to HIV infection. These viruses with sexual transmission in association suggested ASCC is a sexually transmitted tumor. HIV infection changed ASCC patient profile. This kind of tumor committed women in theirs sixties, but nowadays, affects mainly men in the third and forth decades of life. In the United States of America, ASCC was diagnosed in 19/100.000, in the

  2. Análise da dinâmica e estrutura de focos da morte súbita dos citros Dynamics and structure analysis of citrus sudden death foci

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    Waldir C. de Jesus Junior

    2004-08-01

    Full Text Available A morte súbita dos citros (MSC é uma nova e destrutiva doença que afeta laranjeiras doces (Citrus sinensis e algumas tangerineiras (C. reticulata enxertadas em limoeiro 'Cravo' (C. limonia. Sua etiologia e mecanismos de transmissão são ainda desconhecidos e estudos epidemiológicos foram iniciados recentemente. Com o objetivo de caracterizar a disseminação da MSC, foi empregada a técnica de análise da dinâmica e estrutura de focos (ADEF em 166 mapas de distribuição espacial de plantas com sintomas de MSC, provenientes de 51 talhões do norte do Estado de São Paulo e sul do Triângulo Mineiro. Pela ADEF, as epidemias de MSC se iniciam com focos unitários distribuídos de maneira aleatória no talhão. Em talhões com até 2% de incidência, 85% dos focos continham uma planta. Posteriormente, o progresso da doença ocorreu mais pelo aumento de novos focos, que pelo aumento do tamanho dos focos, indicado pelo aumento do número de focos até 18% de incidência e pelo número reduzido de plantas por foco (menos de quatro plantas por foco a 20% de incidência. Na maioria dos casos (71,5%, os focos apresentaram maior expansão na direção da linha de plantio que entre as linhas. A diminuição da compacidade dos focos com o aumento da incidência da MSC sugere que os focos maiores tendem a ser menos compactos e que a disseminação da doença não ocorre de forma homogênea e contínua ao redor da primeira planta afetada. Estes padrões são similares aos padrões de doenças causadas por um agente infeccioso, transmitido por vetores.Citrus Sudden Death (CSD is a new and destructive disease that affects sweet oranges (Citrus sinensis and some mandarins (C. reticulata grafted on Rangpur lime (C. limonia. Its etiology and transmission mechanisms are still unknown, epidemiologic study of the disease was recently initiated. Dynamics and structure analysis of foci technique (DSAF was used to characterize CSD dissemination on 166 spatial

  3. Transmissão do Cowpea mild mottle virus pela mosca branca Bemisia tabaci biótipo B para plantas de feijão e soja Transmission of the Cowpea mild mottle virus by whitefly Bemisia tabaci biotype B for plants of beans and soy

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    Julio Massaharu Marubayashi

    2010-06-01

    Full Text Available A necrose da haste da soja é causada por um vírus do gênero Carlavirus transmitido pela mosca branca Bemisia tabaci, também infectante de feijão e identificado como Cowpea mild mottle virus (CpMMV. Neste trabalho foram realizados testes para determinação do número de moscas-brancas B. tabaci biótipo B necessários para transmissão do vírus em feijoeiro e soja. Na sequência foram realizados dois outros testes, com 10 insetos por planta. Avaliaram-se períodos de acesso à aquisição (PAA de 'Jalo' para 'Jalo', e o efeito de períodos de acesso à inoculação (PAI. Foram visualmente constatados sintomas típicos do carlavírus como mosaico, clareamento de nervuras, necrose sistêmica e redução de crescimento. Houve transmissão do vírus para 'BT-2' de feijão e 'BRS-132' de soja com apenas um inseto por planta, sendo mais eficaz nesta última espécie. A taxa de transmissão do vírus foi maior com o aumento do número de insetos por planta. E o PAA foi determinado após 15' de tempo para aquisição, e o PAI com 5 min e aumentando os períodos de acesso a aquisição e inoculação aumentou-se a taxa de transmissão.The stem necrosis of soybean is caused by a virus of the Carlavirus and transmitted for the whitefly Bemisia tabaci, also infectad of beans and identified as Cowpea mild mottle virus (CpMMV. In this work biotype B for transmission of the virus in beans and soy had been carried through tests for determination of the number of necessary whiteflys B. tabaci. In the sequence two other tests had been carried through, with 10 insects for plant. Periods of access to the acquisition (PAA of 'Jalo' for 'Jalo', and the effect of periods of access to the inoculation (PAI had been evaluated increase. Visually typical symptoms of carlavirus had been evidenced as mosaic, veinclearing, sistemic necrosis and reduction of growth. It had transmission of the virus for 'BT-2' of beans and 'BRS-132' of soy with only one insect for plant

  4. Distribuição de geminivírus nas culturas do tomate e pimentão em doze municípios do Submédio do Vale São Francisco Distribution of geminivirus in tomato and sweet pepper crops in twelve counties of the Lower Basin of San Francisco Valley

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    MIRTES F. LIMA

    2001-03-01

    Full Text Available Em 1996 e 1997, observaram-se sintomas de viroses causadas por geminivírus transmitidos por mosca branca em plantas de tomateiro (Lycopersicon esculentum e pimentão (Capsicum annuum no Submédio do Vale São Francisco, situado nos Estados de Pernambuco e Bahia. De outubro de 1996 a dezembro de 1998, foram coletadas 1.368 amostras foliares de tomateiro e 194 de pimentão, exibindo sintomas similares àqueles causados por geminivírus, em 104 e 16 plantios, respectivamente, de 12 municípios dessa região e em dois municípios vizinhos. A incidência de geminiviroses nas áreas amostradas foi estimada entre 5 e100% para tomate e entre 10 e 20% para pimentão. A detecção de geminivírus nas amostras foi feita por "dot blot" ou "squash blot" utilizando-se sondas heterólogas. Para as amostras de tomate, a sonda foi constituída pelos componentes A completos de dois isolados, um de Bean golden mosaic virus (BGMV do Brasil e outro de Bean golden yellow mosaic virus (BGYMV da Guatemala, enquanto que para pimentão, esta foi constituída apenas por um fragmento do componente A de um isolado de geminivírus de tomate do Distrito Federal. Do total de 1562 amostras analisadas, em 908 (58,1% confirmou-se a presença de geminivírus, sendo 823 (60,2% de tomate e 85 (43,8% de pimentão. A presença de plantas infetadas foi detectada em todos os 120 plantios, com incidência entre 20 e 100%, indicando ampla disseminação de geminivírus nestas culturas no Submédio do Vale São Francisco.In 1996 and 1997, whitefly-transmitted geminivirus symptoms were observed in tomato (Lycopersicon esculentum and sweet pepper (Capsicum annuum plants in the Lower basin of San Francisco Valley, located in the states of Pernambuco and Bahia, Northeastern Brazil. One thousand three hundred and sixty-eight leaf samples of tomato and 194 pepper leaf samples showing similar symptoms to those caused by geminivirus were randomly collected from October 1996 to December 1998 in

  5. Implications of the Structural Change in Dairy Products Trade on Milk Price Paid to Producers in Chile Implicancias del Cambio Estructural en el Mercado de Productos Lácteos Sobre el Precio de Leche Pagado a Productor en Chile

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    Alejandra Engler

    2008-06-01

    Corrección de Error (VEC y análisis de cointegración. Los resultados demuestran que el precio doméstico, y los precios CIF (Cost, Insurance, Freight y FOB (Free On Board están cointegrados, lo que implica que fluctuaciones en los precios de importación y exportación son transmitidos al mercado doméstico. Antes de 2001 el precio CIF era la referencia relevante para los movimientos del precio doméstico, mientras que a partir de 2001 el precio FOB se transformó en la referencia y CIF dejó de ser significativo. Los parámetros del modelo sugieren que la relación de cointegración entre CIF y el precio doméstico para el primer período de análisis (1990-2000 es más robusta que la relación entre FOB y el precio doméstico para el segundo período (2001-2007/3. El coeficiente de elasticidad precio para el vector FOB-precio doméstico tiene un intervalo de confianza amplio, lo cual no permite concluir de manera robusta la dirección y magnitud del efecto del precio FOB sobre el precio pagado a productor en Chile a partir del año 2001.

  6. Etiología y efecto en genotipos del pardeamiento y la brotación anormal asociado con fitoplasmas en tubérculos de papa Etiology and effect in genotypes of browning and abnormal sprout associated to phytoplasmas in potato tubers

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    Margarita Díaz-Valasis

    Full Text Available La punta morada de la papa afecta el rendimiento y la calidad de los tubérculos por el pardeamiento y la brotación anormal asociados con ésta. Esta enfermedad es considerada de origen fitoplásmico, aunque los postulados de Koch no han sido debidamente probados. Esta investigación intenta determinar la etiología del pardeamiento y la brotación anormal en papa; para ello, se transmitieron mediante injerto, aislamientos procedentes de 19 genotipos de papa de campo con cuatro variedades: Sangema que tuvo 52.63% de transmisión, Michoacán que tuvo 42.1%, Norteña con 31.57% y NAU-6 que alcanzó 65.2%. Los esquejes de papas de invernadero con síntomas de punta morada transmitidos por semilla-tubérculo, indujeron síntomas de "popotillo" en jitomate; sin embargo, al volver a injertar en papa no se observaron síntomas en el follaje, tampoco pardeamiento en los tubérculos y no se detectó fitoplasma mediante la técnica de reacción en cadena de la polimerasa. El porcentaje de transmisión para la punta morada de la papa en invernadero fue de 37%. El pardeamiento y la brotación anormal en los tubérculos fueron inducidos por un patógeno infeccioso, trasmitido hasta por seis injertos sucesivos en invernadero. El porcentaje de detección de fitoplasma con polimerasa en follaje y tubérculo de las variedades utilizadas NAU-6, Norteña, Michoacán y Alpha fue de 37.5, 10, 35.7 y 28.6 respectivamente. En chile manzano y mixquic, éste fue de 70.83% y 66.6%, en tomate no se registraron fitoplasmas. Las plantas de chile, puede actuar como hospedante intermediario en la transmisión del patógeno involucrado con esta enfermedad.Potato purple top affects yield and tubers quality by browning and the abnormal sprout associated with it. This disease is considered of phytoplasmic origin, although Koch postulates have not been properly proven. This investigation tries to determine etiology for browning and abnormal sprouting in potato; taking this into

  7. Adolescência, sexualidade e mídia: uma breve revisão da literatura nacional e internacional Adolescencia, sexualidad y medios de comunicación: una breve revisión de la literatura nacional e internacional Adolescence, sexuality and media: a brief revision of national and international literature

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    Raquel de Barros Pinto Miguel

    2007-08-01

    Full Text Available Este trabalho teve como objetivo identificar a produção existente acerca dos temas adolescência, sexualidade e mídia. Para tanto foram realizados levantamentos em bases de dados internacionais e nacionais, utilizando termos descritores relacionados ao assunto. Nas referências encontradas foram identificados os temas mais investigados por seus autores, estando entre eles: papel informativo da mídia, o conteúdo sexual veiculado pela mídia, sua influência na sexualidade do adolescente e relação entre mídia e a preservação do paradigma dominante. Os veículos midiáticos mais explorados nos estudos são a televisão e as revistas. Foi possível também identificar autores de relevância, tanto no âmbito nacional quanto internacional, bem como livros e periódicos de referência na área. Constatou-se a exígua produção acerca desta temática, principalmente quando comparado ao número de trabalhos abordando os temas adolescência e sexualidade, em especial em âmbito nacional.Este trabajo tuvo como objetivo identificar la producción existente respecto a los temas adolescencia, sexualidad y medios de comunicación. Para ello, fueron realizados levantamientos en bases de datos internacionales y nacionales, utilizando términos descriptivos relacionados al asunto. En las referencias encontradas fueron identificados los temas más investigados por sus autores. Entre ellos están: rol informativo de los medios, el contenido sexual transmitido por los medios, su influencia en la sexualidad del adolescente y la relación entre los medios y la preservación del paradigma dominante. Los medios más explorados en los estudios fueron la televisión y las revistas. Fue posible también identificar autores de relevancia, tanto en el ámbito nacional como en el internacional, bien como libros y revistas de referencia en el área. Se constató la escasa producción sobre esta temática, principalmente cuando comparada al número de trabajos que

  8. Estructura de la margen norte del Río Grande, Bardas Blancas, provincia de Mendoza Structure of the northen margin of the Grande river, Bardas Blancas, Mendoza province

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    D.J. Dicarlo

    2007-06-01

    Full Text Available La estructura de Bardas Blancas comprende una región sobreelevada tanto topográfica como estructuralmente, conformada principalmente por estructuras braquianticlinales. Afloran en su núcleo rocas del Grupo Choiyoi y hacia los flancos depósitos sedimentarios jurásicos. El área se encuentra en un frente orogénico no emergente caracterizado por el dominio de zonas de plegamiento, fallas ciegas y el desarrollo de una zona triangular. El estudio de estas estructuras con información de subsuelo y superficie permitió el desarrollo de una sección estructural balanceada basada en el modelo de trishear. La sección se basa en el levantamiento de la estructura a partir del desarrollo de fallas de corrimiento de bajo ángulo con vergencia al este despegadas dentro del basamento. El avance de los bloques de basamento habría transmitido parte del acortamiento a la cubierta sedimentaria formándose la zona triangular de Cerro Doña Juana hacia el este. Dentro de la zona triangular se desarrolla un sistema de duplex de techo pasivo generado a partir de un nivel de despegue inferior y otro superior o retrocorrimiento. El retrocorrimiento y los Grupos Rayoso y Neuquén se encuentran volcados, producto del arrastre originado por un corrimiento de vergencia oriental. Hacia el este del retrocorrimiento las secuencias cretácicas superior y terciarias se encuentran desacopladas estructuralmente del Grupo Mendoza.The Bardas Blancas structure constitutes a topographic height characterized by an important basement anticline. The Choiyoi Group crops out in the middle of the structure and Jurassic sedimentary deposits at both limbs. This structure is the orogenic front at these latitudes and it is characterized by blind thrusts, folding and the development of a triangular zone. The Bardas Blancas structure was studied using surface and sub-surface data and a balanced cross section was constructed using the trishear model. The main structures are explained using

  9. Caracterização de um isolado do Bean rugose mosaic virus (BRMV de Minas Gerais e estimativa de perdas em feijoeiro em infecção simples ou em conjunto com o BCMV Characterization of a Bean rugose mosaic virus (BRMV isolate from Minas Gerais, and yield loss estimate in beans upon single infection and double infection with BCMV

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    Gloria P. Castillo-Urquiza

    2006-10-01

    Full Text Available Plantas de feijão-vagem do cultivar Novirex apresentando mosaico e enrolamento de vagens, sem deformação foliar evidente, foram coletadas em 2002 em Cordisburgo, MG. Estudos preliminares identificaram o vírus como um isolado do Bean rugose mosaic virus (BRMV. Este trabalho relata a caracterização do isolado, por meio de produção e avaliação de anti-soro, determinação da gama de hospedeiros, estudo da transmissão do vírus por besouros crisomelídeos e estimativa de perdas em feijoeiro como resultado de infecção isolada ou em conjunto com o Bean common mosaic virus (BCMV. O roteiro adotado para purificação possibilitou a obtenção de vírus purificado em rendimento satisfatório para a produção de anti-soro. A titulação dos anti-soros foi realizada por ELISA indireto, obtendo-se reações positivas com a diluição máxima testada (1:70.000. Das 22 espécies vegetais utilizadas na gama de hospedeiros, foram infectadas plantas de Chenopodium quinoa e alguns cultivares de feijão e soja, conforme esperado para o BRMV. O isolado de BRMV foi transmitido pelo besouro crisomelídeo Cerotoma arcuata a uma taxa de 33,3%. A infecção simples de feijão 'Ouro Negro' e de feijão-vagem 'Novirex' levou a uma redução do peso das vagens por planta de 3,4% e 84,9%, respectivamente. Infecção mista do BRMV com o BCMV levou a uma redução do peso de vagens por planta de até 70,1% para 'Novirex' e de até 90,8% para 'Ouro Negro'.Bean plants of the cultivar Novirex, showing an atypical pod curling symptom without mosaic or leaf distortion, were collected in 2002 at Cordisburgo, MG. Previous studies identified the virus as an isolate of Bean rugose mosaic virus (BRMV. This work reports on the characterization of the isolate, including its purification and production of a polyclonal antiserum, determination of a partial host range, vector transmission, and an estimate of yield losses due to single or mixed infection with Bean common mosaic

  10. A comunicação na abordagem preventiva do câncer do colo do útero: importância das influências histórico-culturais e da sexualidade feminina na adesão às campanhas Communication in the prevention of cervix uteri cancer: the importance of historical cultural influences and of feminine sexuality in the adhesion to campaigns

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    Luciana Maria Britto da Cruz

    2008-06-01

    Full Text Available Considerando que o câncer cérvico-uterino ainda se apresenta como problema de saúde pública no Brasil e que os programas governamentais de prevenção existentes para esse câncer não alcançaram a meta estimada, visto que a cobertura e o número de atendimentos nas campanhas não atingiram um quarto do estimado na primeira campanha; e, na segunda, o alcance foi inferior à primeira, este artigo se propôs a levantar questões referentes a não adesão de muitas mulheres às campanhas. Para isso, foram ressaltadas a relevância epidemiológica para a abordagem do tema; a importância do corpo para a mulher; e as influências histórico-culturais no comportamento preventivo das mulheres. Dessa forma, foi realizado um estudo teórico descritivo, usando como fonte principal de informações dados do Ministério da Saúde e do Instituto Nacional do Câncer (INCA e como fonte de apoio livros, artigos científicos, periódicos e dissertações que abordam a temática proposta. A fim de discutir possibilidades estratégicas que possam ser eficazes para uma maior adesão feminina às campanhas preventivas, foi considerada a importância da forma de abordagem dos profissionais de saúde, salientando para que atuem correspondendo aos valores culturais apresentados pelas mulheres. Além disso, ressaltou-se a linguagem utilizada nas campanhas de prevenção do câncer do colo do útero, atentando para o fato de que conceitos transmitidos podem endossar valores culturais de abordagem do feminino que dificultam a identificação e a adesão de muitas mulheres.Cervix uteri cancer is still a public health problem in Brazil, but the governmental prevention programs that exist for this type of cancer have not reached the estimated index of covering. The number of people assisted in the campaigns has not reached one quarter of what was estimated in the first campaign and in the second one the number was even inferior. In light of this situation, this article

  11. Epidemiología molecular de la rabia urbana en Colombia, 1994-2004. La rabia en ecosistemas silvestres como amenaza para la población humana

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    Jorge Boshell

    2004-03-01

    por vacunación en 1997 y los virus transmitidos pertenecieron a una única variante genética (variante genética colombiana I. La tercera epizootia, aunque ha disminuido notablemente su magnitud debido a los intensos programas de vacunación y control en la zona, continúa siendo un grave problema de salud pública y en la actualidad los zorros silvestres además de los perros actúan como reservorios y transmisores. Los virus transmitidos en la región Caribe pertenecen a una única variante genética (variante genética colombiana II. Mediante análisis filogenéticos computarizados se llegó a determinar que esta variante ha circulado en Colombia desde los años 1960 y es la variante ancestral a partir de la cual evolucionó la variante genética colombiana I. Un tercer grupo de virus de la rabia que fue caracterizado, corresponde a variantes genéticas características de quirópteros.

    Estas variantes representan una gran amenaza para las poblaciones humanas sin importar el lugar que habiten en el país. Recientemente, alrededor de 14 muertes humanas fueron atribuidas a rabia transmitida por quirópteros en una comunidad indígena del departamento del Chocó.

    En el presente trabajo se destaca y discute la importancia de las técnicas de virología molecular aplicadas a la vigilancia epidemiológica de la rabia.

     

    REFERENCIAS

    1. TORDO N, CHARLTON K., WANDELER A. Rhabdoviruses: rabies. In: Topley & Wilson´s Microbiology and Microbial Infections. L.H. Collier Editors. Arnold. London. 1998: 666-692.

  12. New ecological aspects of hantavirus infection: a change of a paradigm and a challenge of prevention--a review.

    Science.gov (United States)

    Zeier, Martin; Handermann, Michaela; Bahr, Udo; Rensch, Baldur; Müller, Sandra; Kehm, Roland; Muranyi, Walter; Darai, Gholamreza

    2005-03-01

    In the last decades a significant number of so far unknown or underestimated pathogens have emerged as fundamental health hazards of the human population despite intensive research and exceptional efforts of modern medicine to embank and eradicate infectious diseases. Almost all incidents caused by such emerging pathogens could be ascribed to agents that are zoonotic or expanded their host range and crossed species barriers. Many different factors influence the status of a pathogen to remain unnoticed or evolves into a worldwide threat. The ability of an infectious agent to adapt to changing environmental conditions and variations in human behavior, population development, nutrition, education, social, and health status are relevant factors affecting the correlation between pathogen and host. Hantaviruses belong to the emerging pathogens having gained more and more attention in the last decades. These viruses are members of the family Bunyaviridae and are grouped into a separate genus known as Hantavirus. The serotypes Hantaan (HTN), Seoul (SEO), Puumala (PUU), and Dobrava (DOB) virus predominantly cause hemorrhagic fever with renal syndrome (HFRS), a disease characterized by renal failure, hemorrhages, and shock. In the recent past, many hantavirus isolates have been identified and classified in hitherto unaffected geographic regions in the New World (North, Middle, and South America) with characteristic features affecting the lungs of infected individuals and causing an acute pulmonary syndrome. Hantavirus outbreaks in the United States of America at the beginning of the 10th decade of the last century fundamentally changed our knowledge about the appearance of the hantavirus specific clinical picture, mortality, origin, and transmission route in human beings. The hantavirus pulmonary syndrome (HPS) was first recognized in 1993 in the Four Corners Region of the United States and had a lethality of more than 50%. Although the causative virus was first termed in

  13. Capacidad bactericida de Bifidobacterium sp. aislada de leche materna y de heces de neonatos, frente a los principales causantes de enfermedades transmitidas por alimentos Antibiotic activity of Bifidobacterium sp. isolated from breast milk and newborn faeces, against the main causes for foodborne illnesses

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    María Consuelo Vanegas

    2010-12-01

    heces de neonatos, por lo cual se confirmó que este microorganismo es microbiota humana (2. Conclusión. Se concluye que, debido a la gran capacidad antagonista de la mayoría de las bacterias aisladas, éstas pueden estar cumpliendo una importante función protectora en el recién nacido, en particular las cepas Bif 013 y Bif 023 aisladas de materia fecal. Estos microorganismos deben continuar siendo estudiados para definir su potencial probiótico. También, se pueden evaluar para bioconservación en la industria y contra patógenos transmitidos por alimentos.Introduction. The microbiota in the human gastrointestinal tract contains beneficial microorganisms for human health, which contribute to the regulation of colonic function and inhibition of some intestinal pathogens growth. Bifidobacterium sp. isolated from newborns and breast milk are used as probiotic microorganisms, which are useful in the prevention of infectious diseases including foodborne illnesses. Objective: To isolate and identify human Bifidobacterium sp. and to determine its antibiotic activity against important pathogens which cause foodborne illnesses in Colombia and the world. Materials and methods. 17 breast milk samples and 19 meconium and newborn faeces samples were collected from different hospitals in Bogotá. 26 presumptive Bifidobacterium strains were identified at genus level by PCR 16-23S; some strains were identified at species level by nucleic acid sequencing. The antagonistic activity of 26 Bifidobacterium sp. strains was tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Listeria monocytogenes ATCC 7644 and E. coli O157:H7 ATCC 35150. For the strains that showed a greater antibiotic activity, the inhibitory compound was analyzed using disk diffusion tests. Results. All strains amplified the expected band for genus confirmation. Strains Bif 023 and Bif 013 were identified by DNA-sequencing as Bifidobacterium breve, with 97

  14. Transmissão de begomovírus de plantas daninhas para tomateiros pela mosca-branca Begomovirus transmission from weeds to tomato by the whitefly

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    A.K.F. Silva

    2010-01-01

    Full Text Available Dentre numerosas enfermidades do tomateiro (Lycopersicon esculentum, destacam-se as viroses causadas por begomovírus, os quais são transmitidos pelo vetor Bemisia tabaci biótipo B. Na Chapada da Ibiapaba-CE, os begomovírus têm sido encontrados em várias áreas onde o tomateiro é cultivado, causando sérios danos à produção. Este trabalho teve por objetivos investigar a transmissão de begomovírus a partir de tomateiros infectados para plantas daninhas e verificar seu retorno das plantas daninhas para o tomateiro. Mudas sadias de tomateiro 'Santa Clara' e das plantas daninhas bredo-de-espinho (Amaranthus spinosus, caruru-de-mancha (Amaranthus viridis, mentrasto (Ageratum conyzoides e picão-preto (Bidens pilosa foram submetidas à inoculação por dois métodos: com o inseto vetor e por enxertia. Após 15 dias, realizou-se a extração do DNA de amostras foliares dos tomateiros e das espécies daninhas inoculadas. A PCR realizada com oligonucleotídeos degenerados e específicos para begomovírus revelou que na transmissão com o vetor as quatro espécies de plantas daninhas foram infectadas com o begomovírus do tomateiro, enquanto que, por enxertia, apenas o picão-preto foi infectado. O retorno do vírus das plantas daninhas para o tomateiro foi também observado nos dois casos. Percentuais de 70, 50, 20 e 12,5% de transmissão para os tomateiros ocorreram quando o vetor adquiriu o vírus em mentrasto, bredo-de-espinho, picão-preto e caruru-de-mancha, respectivamente. Na enxertia, a transmissão viral para os tomateiros ocorreu apenas quando se empregaram seções de bredo-de-espinho e de picão-preto infectados. As espécies daninhas investigadas demonstraram ser hospedeiras alternativas do begomovírus de tomate da região e, em condiçõs de campo e na presença do vetor, podem constituir importantes fontes do begomovírus para a hortaliça.The viruses caused by begomovirus are considered the most important virus diseases

  15. Trastornos de la reactividad al dolor Pain reactivity disorders

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    L. Vicente-Fatela

    2004-02-01

    Full Text Available La función protectora del dolor es bien conocida. Existen algunas enfermedades que cursan con la ausencia de la percepción dolorosa y conllevan a múltiples problemas para los pacientes afectados. En los trastornos de la reactividad al dolor se pueden distinguir trastornos congénitos y adquiridos. Dentro de los congénitos se incluyen dos cuadros clínicos bien diferenciados: la insensibilidad congénita al dolor o analgesia congénita y la indiferencia congénita al dolor. En el primer caso el estímulo doloroso no es transmitido adecuadamente al sistema nervioso central debido a un defecto en las vías sensitivas; mientras que en el segundo la vía sensorial está integra pero el paciente no identifica el estímulo doloroso como desagradable. Actualmente la insensibilidad congénita al dolor se encuadra dentro de un grupo de neuropatías hereditarias llamadas sensitivo-autonómicas, con afectación de la sensibilidad dolorosa, en relación con la implicación de las fibras nerviosas mielínicas pequeñas y amielínicas, vehículos de la mayor parte de la sensibilidad dolorosa, así como de las fibras autonómicas. Se distinguen cinco tipos de neuropatía hereditaria sensitivo-autonómica, que se describen en el texto, existiendo alteración de la percepción dolorosa en todas ellas, aunque con mayor grado en alguna de ellas como en el caso de la neuropatía hereditaria sensitivo-autonómica tipo IV o insensibilidad congénita al dolor con anhidrosis. Clínicamente en estas neuropatías se pueden producir lesiones a diversos niveles como consecuencia de la pérdida de la sensibilidad dolorosa. Las alteraciones que se asocian con mayor frecuencia son: fracturas múltiples, articulaciones de Charcot, dismetría de extremidades inferiores, osteomielitis, artritis séptica, luxaciones, autoamputaciones, automutilaciones y escoliosis progresiva. Es necesario un tratamiento multidisciplinar y especializado junto con la colaboración paterna para

  16. Genealogia feminina: diálogo silencioso entre gerações - Female genealogy: silent dialogue between generations

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    Flávia Obino Corrêa Werle, Brasil

    2014-05-01

    perspectiva de nuevos enfoques parala Historia dela Educación. Considera a la memoria, tanto como un recurso, así como una presencia en los objetos y en la cultura material. Analiza un objeto guardado y transmitido de generaciones en generaciones, para ser usado por las mujeres de una misma familia a la hora del parto. Discute sobre el tema de la formación humana en espacios de relaciones familiares en modalidades no escolarizadas y cuestiona lo que las hermanas se enseñan unas a otras, que aprendizajes surgen en las relaciones entre mujeres que son vecinas, amigas, primas, tías, sobrinas, hijas, madres, abuelas.Palabras-clave: historia de la educación, tradición, memoria.GÉNÉALOGIE FEMININ: DIALOGUE ENTRE LES GENERATIONSRésuméLa méthodologie de cet article, développé le long des lignes de l'histoire culturelle, une approche biographique narrative. Parmi les autres concepts, il aborde la tradition, l'innovation, la généalogie féminine dans la perspective de nouvelles façons de se concentrer sur l'histoire de l'éducation. Il considère la mémoire à la fois comme un discours et une présence dans les objets et la culture matérielle. Analisé un objet stocké et doué de génération en génération, pour être utilisé par les femmes d'une même famille au moment de l'accouchement. Il problématise tle sujet de la formation humaine dans des espaces de relations familiales et dans les modes non-scolarisés, et demande à ce que les sœurs enseignent l'autre, ce qui se produit apprentissages dans les relations entre les femmes qui sont des voisins, des amis, cousins, cousines, sœurs, nièces. Mots-clé: histoire de l'éducation, de la tradition, la mémoire.

  17. Una aportación al estudio del cuento fang de Guinea Ecuatorial en lengua española / A CONTRIBUTION TO THE STUDY ABOUT THE FANG TALES FROM EQUATORIAL GUINEA IN SPANISH LANGUAGE

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    María Teresa Abaga Envó

    2009-06-01

    Full Text Available Resumen: Una lectura sobre los estudios narratológicos revela que el acto narrativo es un hecho consustancial al ser humano. “Somos el homo sapiens, porque somos el homo narrans”, diría el académico José María Merino (2002: 57. De las estructuras narrativas, el cuento oral tradicional se alza como la forma más antigua. Tan antigua y presente en todos los pueblos que componentes semánticos y formales se repiten en relatos de distintas culturas. Tales aspectos constituyen el objeto de análisis de este repertorio de cuentos fantásticos de Guinea Ecuatorial transmitidos en lengua española. En dicho entorno, el cuento oral fang se manifiesta como un arte viviente. No es un evento pensado sólo para niños; toda la comunidad participa de esta “fiesta” que se verá enaltecida por la presencia del canto. Dada la escasa proliferación de estudios del relato oral africano y en particular del guineano, se hace imperativo partir de estudios occidentales, lo cual nos desliza hacia el terreno del comparativismo. Sin embargo, no siempre dichos estudios son confirmados en esta investigación que concluye con las siguientes tesis: el criterio verdad y ficción al que se recurre para delimitar ciertas narraciones tradicionales europeas queda difuminado; uso irregular de las fórmulas de abertura y cierre; la asignación del fin lúdico de los cuentos fantásticos se acompaña del didáctico y la pretendida universalización del método estructuralista formal de Vladimir Propp, expuesta en Morfología del cuento (1987, se vislumbra inoperante, pues quedan reducidas las treinta y una funciones y la sucesión lineal de las mismas. Los temas y los motivos, arropados por el espíritu de la cultura donde emergen, son similares a los identificados en compilaciones españolas o internacionales. Finalmente, la naturaleza oral tradicional del repertorio queda patente a través de las nueve “Leyes Épicas” establecidas por Axel Orlik (1965: la Ley de

  18. Frequency of antibodies to Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burdgorferi in cattle from the northeastern region of the state of Pará, Brazil Freqüência de anticorpos para Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax e Borrelia burgdorferi em bovinos do nordeste do Estado do Pará, Brasil

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    Daniel S. Guedes Junior

    2008-06-01

    , por meio do ensaio de imunoadsorção enzimática (ELISA, a freqüência de anticorpos para Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax e Borrelia burgdorferi em bovinos da região nordeste do Estado do Pará, Brasil. Amostras de soro de 246 vacas dos municípios de Castanhal e São Miguel do Guamá foram usadas. ELISAs com antígeno bruto foram utilizados para detector anticorpos contra todos os agentes, exceto para A. marginale, para o qual um ELISA indireto com proteína principal de superfície 1a (MSP1a foi usado. As freqüências de bovinos soropositivos foram: B. bigemina - 99,2%; B. bovis - 98,8%; A. marginale - 68,3%; T. vivax - 93,1% and B. burgdorferi -54,9% As freqüências de bovinos soropositivos para B. bovis e B. bigemina sugerem uma alta taxa de transmissão desses organismos por carrapatos, na região estudada, a qual pode ser classificada com sendo de estabilidade enzoótica para os hemoparasitos. A baixa freqüência de bovinos soropositivos para A. marginale pode ser atribuída a uma menor sensibilidade do ELISA com antígeno recombinante, ou uma menor taxa de inoculação da riquétsia pelos carrapatos, quando comparada àquelas observadas para Babesia sp. A alta freqüência de bovinos soropositivos para T. vivax indica que esse hemoprotozoário é prevalente em rebanhos do nordeste do Estado do Pará. O percentual de animais com anticorpos homólogos para B. burgdorferi indica a presenças deste espiroquetídeo transmitido por carrapatos na população de bovinos da região estudada.

  19. Malaria infection and the anthropological evolution Infecção por Malária e evolução antropológica

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    Sergio Sabbatani

    2010-03-01

    malária exerceu papel biológico chave, influenciando até o desenvolvimento antropológico. Os Plasmódios causadores da malária desenvolveram dois tipos de evolução, segundo um ponto de vista biológico, e filogenético. Em particular, os Plasmódios vivax, malariae, e ovale poderiam ter coevoluído com a espécie humana ou ter atingido esta durante as fases mais antigas da evolução do gênero HOMO. Por outro lado, o Plasmódio falciparum teria sido transmitido aos humanos por macacos num período mais recente, provavelmente entre o fim da Idade Mesolítica e o início da Neolítica. Os Autores mostram evidências biomoleculares, tanto diretas como indiretas, de infecção por malária, observada em indivíduos enterrados, reportando ao Mundo Antigo, e trazidas à luz no decorrer de escavações arqueológicas em alguns sítios relevantes Mediterrâneos. Nesta revisão da literatura, os autores organizam evidências modernas. Confirmam o papel da malária ao afetar a evolução das populações no Mediterrâneo. As pessoas que moram nas várias regiões do Mediterrâneo foram progressivamente influenciadas pela malária, ao longo do avanço desta doença. Além disso, as populações afetadas desenvolveram adaptações culturais, alimentares, e de comportamento, contribuindo para uma redução no risco da doença. Entretanto, pode ser que ambas modificações (as biológicas e as dos hábitos usadas pelos Plasmódios da malária, favoreceram a emergência de grupos de pessoas com maior resistência contra a malária. Todos estes fatores considerados reduziram o impacto demográfico, influenciando de uma maneira favorável o desenvolvimento em geral, e o crescimento da civilização.

  20. Soroprevalência e perfil imunofenotípico de células linfóides T em indivíduos soropositivos para o vírus linfotrópico de células T humanas Seroprevalence and immunophenotypic profile of T lymphocyte cells in human T lymphotropic virus seropositive individuals

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    Geane F. de Sóuza

    2003-03-01

    Full Text Available O vírus linfotrópico de células T humana (HTLV é transmitido por transfusões, uso compartilhado de agulhas contaminadas, aleitamento e contato sexual. A prevalência varia de acordo com a região geográfica, grupo racial e população estudada. Cerca de 1% a 4% dos indivíduos infectados desenvolvem algum tipo de doença em decorrência da infecção. É reconhecida a associação entre o HTLV-I e leucemia de células T do adulto e paraparesia espástica tropical (PET. Embora a maioria dos portadores permaneça assintomática, existem evidências de comprometimento funcional da resposta imune celular. Os objetivos desse trabalho foram avaliar a prevalência de soropositividade para HTLV-I/II na população de doadores de sangue do HEMOCE e analisar o perfil imunofenotípico de células linfóides circulantes em 26 doadores soronegativos, 11 soropositivos para HTLV-I sintomáticos e 24 assintomáticos, comparando-os entre si. A prevalência da soropositividade para HTLV-I/II foi de 0,66%. No grupo de indivíduos contaminados pelo HTLV-I houve predomínio do sexo feminino e a maior média de idade. O grupo soropositivo apresentou menor valor de hemoglobina e o grupo sintomático evidenciou contagem de neutrófilos significativamente mais elevada. A contagem média de linfócitos não diferiu entre os grupos. A análise imunofenotípica mostrou que os valores médios de células CD3+, CD4+, CD8+ e relação CD4/CD8 não diferiram significativamente entre os grupos. Uma elevação de células CD8+ no grupo soropositivo foi observada embora não alcançasse significância estatística. A ativação de linfócitos CD8+ está envolvida na patogênese das doenças associadas ao HTLV-I. A definição do valor preditivo desse achado requer confirmação posterior.Human T lymphotropic virus (HTLV can be transmitted by transfusions of cellular blood products, shared use of contaminated syringes, breast feeding and sexual intercourse. The prevalence of

  1. Um estudo sobre os significados de consumo associados ao corpo feminino em peças publicitárias de suplementos alimentares A study on the meanings of consumption associated to the female body on dietary supplements' ads

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    João Felipe Rammelt Sauerbronn

    2011-04-01

    Full Text Available O presente artigo buscou analisar os significados atribuídos aos corpos femininos e transferidos através de propagandas de suplementos alimentares. O corpo se transformou em objeto de pesquisa de pesquisadores interessados em explorar a construção de significados de consumo de diversas áreas. Sua expressão como mercadoria já foi apontada por pesquisadores como Malysse 2007 que propôs a objetificação do corpo e Goldenberg 2007 que construiu o conceito de corpo como capital. Segundo MacCracken 2003 , a publicidade atua na transferência de significados, desde o mundo culturalmente constituído até os indivíduos. As nove peças publicitárias coletadas foram analisadas com base no método de análise do discurso publicitário proposto por Pinto 2002 . As funções de mostração construção do universo do discurso , de interação estabelecimento de vínculos socioculturais e de sedução distribuição de afetos positivos e negativos serviram como direção para que os pesquisadores buscassem compreender as formas de transferência de significado do corpo feminino através das peças publicitárias de suplementos alimentares. Alguns componentes do discurso publicitário foram comuns a todas as peças coletadas e evidenciaram o corpo como um objeto que pode ser modificado, ter suas medidas alteradas a partir do que é transmitido como desejável um corpo sem gordura, magro e firme . Entretanto, puderam ser diferenciadas quatro categorias de discurso de transferência de significado do corpo. A primeira categoria de peças publicitárias coloca o corpo como objeto de poder e o supervaloriza como objeto. Nessa categoria, a interação com os indivíduos é baseada no status que o corpo pode oferecer ao sujeito e as emoções envolvidas tratam de elevar a auto-estima do indivíduo. A segunda categoria congrega peças publicitárias que apresentam o corpo como local da ação do produto. A interação, nesse caso, é baseada em técnica e

  2. Assédio moral no trabalho e suas representações na mídia jornalística Acoso moral en el trabajo y sus representaciones en el medio periodístico Representations of workplace psychological harassment in print news media

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    Andréia De Conto Garbin

    2012-06-01

    Full Text Available OBJETIVO: Analisar os discursos sobre assédio moral veiculados na mídia jornalística impressa. PROCEDIMENTOS METODOLÓGICOS: Estudo documental referente ao assédio moral no trabalho, no qual foram analisadas as matérias jornalísticas veiculadas em três jornais de grande circulação do estado de São Paulo, no período de 1990 a 2008. A partir da metodologia de análise do discurso foram reconhecidas as práticas discursivas que configuram o fenômeno do assédio moral na sociedade atual, as explicações para sua ocorrência e a repercussão para a saúde dos trabalhadores. ANÁLISE DOS RESULTADOS: O surgimento do tema nos veículos de comunicação deu-se por meio da divulgação de livros, de produções acadêmicas e de legislações. Ocorreu em editorias que tratam de assuntos gerais e, posteriormente, migrou para as editorias de emprego e/ou de caráter econômico-financeiro. Os discursos de natureza indenizatória, de precaução empresarial e as estratégias de enfrentamento são amplamente difundidos. A promoção da saúde se esvai pela lógica patrimonial. Há um espaço permissivo nas organizações para prática do assédio moral, potencializando os conflitos para atingir as metas e resultados. Indiferença, constrangimentos, desqualificações e ridicularizações foram comuns nas matérias. CONCLUSÕES: As explicações sobre o assédio tendem a uma interpretação psicológica do fenômeno, acentuando o caráter individualista e minimizando uma abordagem coletiva. Os discursos banalizam o assédio ao criarem caricaturas para os atores envolvidos. O conteúdo psicológico e a estigmatização produzem sentido na sociedade, contribuindo para naturalizar o assédio moral no trabalho e banalizar a violência no trabalho.OBJETIVO: Analizar los discursos sobre el acoso moral transmitidos en el medio periodístico impreso. PROCEDIMIENTOS METODOLÓGICOS : Estudio documental concerniente al acoso moral en el trabajo, en el cual

  3. Epidemiología molecular de la fiebre amarilla en Colombia: análisis filogenético

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    Jorge Boshell

    2004-03-01

    Full Text Available

    El virus de la fiebre amarilla pertenece a la familia Flaviviridae, género Flavivirus, y es el responsable de una enfermedad hemorrágica aguda que aún hoy en día afecta a más de 200.000 personas al año en regiones tropicales de África y Suramérica. El virus puede ser transmitido de un humano infectado a un humano susceptible por el mosquito doméstico Aedes aegypti o de primates no humanos a primates humanos por vectores selváticos, principalmente del género Haemagogus spp. (1-5. La infección con el virus puede ser asintomática o cursar con fiebre moderada en 40% a 65% de los pacientes, hasta convertirse en una infección fatal fulminante caracterizada por postración, daño hepático, renal, cardíaco y choque en 35-60% de los casos; la letalidad en casos graves varía entre 20% y 50%.(1-2.

    Aunque el virus puede ser detectado mediante técnicas serológicas (MAC-ELISA o virológicas (aislamiento viral en células o en ratón (1,6, estos sistemas no ofrecen ninguna información acerca del origen epidemiológico de las cepas analizadas, ni ponen en evidencia relaciones genéticas inter-cepa; por esta razón y para mejorar la sensibilidad de la clasificación viral, hemos realizado un análisis filogenético de las cepas del virus fiebre amarilla que han circulado en nuestro país y que han generado brotes epidémicos durante el último año.

    Se procesaron 20 muestras de pacientes (incluyendo suero y tejido hepático con diagnóstico previo de fiebre amarilla, obtenidas entre el 1º de junio de 2003 y el 20 de enero de 2004 y 4 muestras obtenidas entre 1954 y 1973, las cuales fueron tratadas con Trizol LS para la extracción del ARN; mediante RT-PCR utilizando iniciadores previamente reportados (6, se amplificaron

  4. DE LA RABIA HUMANA DE ORIGEN CANINO Y OTRAS VERGÜENZAS

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    Nelson Alvis G

    2006-12-01

    por rabia humana del período(17casos; seguida de la región occidental (5 casos, la Orinoquia (3 casos, la Amazonía (2casos y la Región Central, un sólo caso en el 2003. Es decir todo estaba anunciado.Los muertos se presentaron como consecuencia de la mordedura de perros infectados con elvirus de la enfermedad, en diciembre de 2006. El Instituto Nacional de Salud confirmó loscasos y estimó éste como el brote mas grave, luego del ocurrido entre mayo y julio del 2004en el que se notificaron 14 casos en el Bajo Baudó, por mordedura de murciélago. Cabeaclarar que se trata de un tipo de virus distinto al transmitido por caninos.Según informes de la Secretaría de Salud de Santa Marta, al parecer las víctimas fueronmordidas en septiembre. La primera falleció el 5 de noviembre, la segunda 20 días despuésy la tercera el 16 de enero. Uno de los indicadores a partir de los cuales deben generarsealertas es la presencia de rabia animal. Ya para el 2005, el departamento del Magdalenamostraba el mayor número de casos reportados en el país: 18 de los 97 registrados.En el informe se destaca a pie de pagina: “El grupo evaluador, en virtud de razonesepidemiológicas, operativas y políticas considera necesario resaltar la urgencia de estarecomendación considerando que la situación de rabia transmitida por el perro en losDepartamentos de la Costa es un asunto que requerirá la intervención directa de las masaltas autoridades nacionales para evitar casos humanos prevenibles que se ven comoaltamente probables en un futuro próximo de persistir la situación actual. El virus de la rabianecesita de la existencia de perros no vacunados para sostenerse en la población caninae inesperadamente afectar a una persona si no se la trata oportunamente. El deficienteesquema de vacunación que se verificó en los departamentos de la costa, sumado asituaciones ecológicas particulares y a deficiencias en el control de focos, permite al viruscircular y manifestarse – de cuando en

  5. Las atarazanas de Sevilla: entre la construcción y la arquitectura

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    Molino Barrero, Julia

    2005-06-01

    para acondicionarlo a la habitabilidad genérica sea cual fuese su futuro destino.La tercera intervención que propusimos y llevamos a cabo, se centró en la transformación de la antigua Fundición de Artillería en una nueva sala que denominamos “Sala Fundición Atarazanas”. El objeto del proyecto consistió en aclimatar el espacio, dotándolo, mediante una nueva cubierta adaptada, a las exigencias de confort climático, acústico y lumínico; y de unos cerramientos de los arcos que no mermaran la identidad de los mismos ni la continuidad perceptiva del conjunto.Sevilla es un lugar de calor en verano y de humedad en invierno. Era necesario airear, ventilar y acondicionar con frío y calor la sala. El aire acondicionado de la sala se distribuye por la espina dorsal de la cubierta. El retorno bajo las gradas se conecta y hace funcionar de nuevo la vieja chimenea.Estas nuevas formas, estos materiales de hoy que permiten la palabra y el silencio, el aislamiento y la continuidad, la contemplación y la actividad, deben ser aceptados por las Atarazanas de modo natural, y están propuestos desde la enseñanza que el propio edificio nos ha transmitido día a día desde su origen.La cuarta intervención fue la reparación de la Buhardilla del Cuerpo de Cabecera.Solucionar problemas esenciales, entender la verdadera entidad de las Atarazanas -entre la construcción y la arquitectura- y disponer el espacio para su uso, ha sido la constante guía de nuestras obras, y al abrirla al uso público se contribuye a su rehabilitación integral. Quizás sea ésta la razón principal de nuestro proyecto.

  6. Sinais clínicos, distribuição das lesões no sistema nervoso e epidemiologia da raiva em herbívoros na região Nordeste do Brasil Clinical signs, distribution of the lesions in the central nervous system and epidemiology of rabies in northeastern Brazil

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    Everton Ferreira Lima

    2005-12-01

    Full Text Available Descrevem-se 24 surtos de raiva em bovinos (25 casos, 4 em eqüinos (5 casos, 2 em caprinos (2 casos e 2 em ovinos (4 casos. Todos os surtos ocorreram na Paraíba, exceto um em eqüinos que ocorreu no Rio Grande do Norte. Todos os surtos, com a exceção de um em ovinos, foram transmitidos provavelmente por morcegos hematófagos; no entanto, não se descarta a possibilidade de transmissão por raposas (Dusicyon vetulus. Os sinais clínicos foram representativos da localização das lesões no sistema nervoso central (SNC. Em bovinos os sinais eram, principalmente, da forma paralítica, causados por lesões da medula, tronco encefálico e cerebelo; mas alguns animais apresentaram depressão, excitação e outros sinais associados a lesões cerebrais. Três dos 5 eqüinos apresentaram sinais de lesões cerebrais e 2 apresentaram a forma paralítica. De 4 ovinos e 2 caprinos, 4 apresentaram a forma paralítica, mas em um caprino e um ovino os sinais foram predominantemente de lesões cerebrais. Todos os animais afetados, exceto um caprino, tiveram um curso clínico de 2-8 dias. As únicas lesões macros-cópicas observadas foram a dilatação da bexiga em 4 bovinos e a dilatação da ampola retal em 2. Dois cavalos apresentaram lesões da pele causadas por traumatismos. As lesões histológicas foram de encefalomielite e meningite difusa não supurativa. Nos eqüinos e em um caprino com evolução clínica de 35 dias as lesões foram mais severas, observando-se necrose neuronal, neuronofagia e presença de esferóides axonais. Corpúsculos de Negri foram observados em 87% (20/23 dos bovinos examinados e em 83% (5/6 dos ovinos e caprinos. Nessas 3 espécies os corpúsculos foram mais freqüentes no cerebelo, mas ocorreram também no tronco encefálico, medula e cérebro. Em cavalos, corpúsculos de Negri foram menos numerosos, sendo observados somente no córtex de um animal e no córtex e hipocampo em outro. No gânglio trigeminal, as les

  7. Modelos animales de fiebres hemorrágicas humanas producidas por arenavirus

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    Juan D. Rodas

    2004-03-01

    Full Text Available

    En ciertas áreas endémicas para el virus de la fiebre de Lassa en África, este agente representa una amenaza importante para la salud pública. El agente etiológico de la fiebre de Lassa es transmitido de ratas a humanos a través de la sangre o de la exposición de las mucosas a aerosoles y causa cerca de 300.000 casos cada año. El virus de la coriomeningitis linfocítica (LCMV, estrechamente relacionado con el virus de la fiebre de Lassa, produce variados resultados después de su inoculación en primates. En nuestro laboratorio hemos empleado la especie Macaca mulata de mono Rhesus como modelo de fiebre de Lassa en el hombre, usando una variante patogénica (WE y una no patogénica (Armstrong de LCMV.

    Nuestros objetivos fueron determinar el resultado de la infección en monos después de la exposición oral y el desafío letal posterior, caracterizar la respuesta inmune protectora y probar protección cruzada usando las dos cepas virales mencionadas.

    El experimento inicial utilizando las vías de inoculación intravenosa (experimental clásica y oral (hipotética permitieron describir cómo, la vía oral. Produce una “aparente” infección asintomática, mientras la intravenosa es uniformemente letal; permitiendo concluir por lo tanto, que la ruta de exposición influencia dramáticamente el resultado clínico de la infección.

    En un segundo experimento, dos dosis altas (107 o 108 unidades formadoras de placa de la cepa WE del virus LCMV fueron

  8. Situación y tendencias epidemiológicas de la infección por VIH/SIDA en Colombia

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    Ricardo Luque Núñez

    2004-03-01

    heterosexual, 28,3% homosexual y 16,8% bisexual, para un total de 96% transmitidos por vía sexual, 3,1% perinatal, 0,67% transfusional, 0,11% en usuarios de drogas intravenosas y 0,12% por otras formas. Por otro lado, en el estudio centinela realizado en el año 2000 (2, se encontraron prevalencias por encima de lo esperado en departamentos de la costa atlántica, lo que sugiere una tendencia a una epidemia generalizada en estos sitios. Las mujeres representan el 18% de los casos, pero este número continúa creciendo y la relación hombre-mujer sigue siendo cada vez más cercana, con una relación de 3:1 promedio en el país, que en algunos departamentos puede ser de 2:1. El 52.86 % de los casos por VIH se presenta en personas entre los 15 y los 35 años, lo que quiere decir que este grupo poblacional, probablemente, se infectó antes de los veinte años de edad.

    La tendencia de la epidemia es creciente y las curvas no parecen aplanarse de la forma como se ha logrado en otros países. Según las proyecciones, si las intervenciones no logran su efecto o no se les da la sostenibilidad requerida, para el año 2010 tendremos una prevalencia cercana al 1.5%, lo que representará un número estimado de 800.000 casos.

     

    REFERENCIAS

    1. INSTITUTO NACIONAL DE SALUD. Estudio de prevalencia al VIH con HSH en Bogotá, Liga Colombiana de Lucha contra el SIDA, 2000.

    2. INSTITUTO NACIONAL DE SALUD. Estudio Centinela Nacional, 2000.

  9. Correspondencia

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    Salim Mattar

    2006-06-01

    émicas con co-circulación de Flavivirus en el Caribe colombiano» publicado en el volumen 36 N°3, p. 179-185 de Colombia Médica, por lo que significan sus hallazgos para la investigación epidemiológica con este virus. Sin embargo, se requiere hacer una aclaración sobre un error en la taxonomía de los virus que aparece en el documento. En años anteriores, se usaba el término arbovirus para definir a aquellos que son capaces de infectar y replicarse en insectos (ARthropod BOrned VIRUS. El término sigue siendo usado coloquialmente para definir aquellos virus que pueden ser transmitidos por insectos; sin embargo, no se ajusta a los criterios actuales de taxonomía definidos por el Internacional Committee on Taxonomy of Viruses (ICTV, que usa fundamentalmente el tipo de ácido nucleico como el criterio central en la clasificación. De esta manera, los miembros de los Arbovirus fueron incluidos en diferentes órdenes y familias, sin tener en cuenta las consideraciones eco-epidemiológicas. En el mencionado artículo, en varias ocasiones se incluye al virus de encefalitis equina venezolana (VEEV como parte de la familia Flaviviridae, lo cual es incorrecto, pues este virus, aunque está incluido en el Taxa de virus con ARN de sentido positivo de cadena sencilla (como los flavivirus, pertenece a la familia Togaviridae, que cuenta con dos géneros, Alphavirus (VEEV y otros y Rubivirus (rubéola. Por esta misma razón, se podría considerar que el hallazgo de un suero doblemente positivo para VEEV y dengue, realmente podría estar representando un caso antiguo de dengue también con antecedentes de contacto con VEEV, y no una reactividad cruzada como se sugiere en el texto, pues no se han informado epítopes compartidos entre ambas familias de virus. Agradezco su atención, cordialmente, Jaime E. Castellanos e-mail: castellanosjaime@unbosque.edu.co Director Instituto de Virología, Universidad El Bosque Bogotá, Colombia Señor Editor: En respuesta a la aclaración que hizo

  10. Des lieux avec lesquels penser, des livres auxquels penser Places to think with, books to think about Lugares para pensar con ellos, Libros para pensar en ellos

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    Anders Burman

    2012-06-01

    source and supposed reliability has interesting implications for the current process of decolonization of the Bolivian University and the recent establishment of ‘indigenous universities’ as integral parts of the decolonizing state politics launched by the Evo Morales administration. In this paper I pose the question that if books and lectures are basically about the opinions and judgments of particular individuals, and proper knowledge is to be gained only in the experiential, non-linguistic, inter-relational dealings with and in the world, wouldn’t a project aimed at decolonizing knowledge and decolonizing the University precisely by way of books and lectures, i.e. a logocentric project of decolonization, be a venture fated to reproduce the colonial epistemological asymmetries of knowledge production? On the one hand, then, this paper scrutinizes the problems linked to the ‘siwsawi’ nature of conventional (colonial academic knowledge in relation to a critical process of decolonization. On the other hand, it explores the ‘ukamaw’ nature of experiential knowledge and the prospects for this kind of knowledge to lay the fundaments for a decolonial epistemological transformation of the Bolivian University. Fundamentally, this paper addresses issues such as what it means to know, what knowledge is, and what it means to be a knowing and knowledgeable subject in the Bolivian Andes today, in a context where subalternized traditions of thought gain new urgency in new educational and political dynamics and where different visions and claims of truth coexist, coalesce and collide.Muchos hombres y mujeres Aymara afirman que el saber humano, transmitido por la lengua, solo es un puro ‘siwsawi’, i.e. palabras, opiniones, puntos de vista, juicios de individuos particulares. Entonces, se trata de un saber particular; es un conocimiento que viene de la opinión de algunas personas, nada más. Esto es, de manera significativa, diferente del saber no-linguístico, del