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Sample records for bullous keratopathy corneas

  1. Extracellular matrix alterations in human corneas with bullous keratopathy

    DEFF Research Database (Denmark)

    Ljubimov, A V; Burgeson, R E; Butkowski, R J; Couchman, J R; Wu, R R; Ninomiya, Y; Sado, Y; Maguen, E; Nesburn, A B; Kenney, M C

    1996-01-01

    PURPOSE. To uncover abnormalities of extracellular matrix (ECM) distribution in human corneas with pseudophakic and aphakic bullous keratopathy (PBK/ABK). METHODS. Indirect immunofluorescence with antibodies to 27 ECM components was used on frozen sections of 14 normal and 20 PBK/ABK corneas...

  2. Results of the Application of Phototherapeutic Keratectomy in the Elderly with Bullous Keratopathy

    Directory of Open Access Journals (Sweden)

    Osbel Alfonso Sánchez

    2012-11-01

    Full Text Available Background: Many countries have conducted studies on the benefits of phototherapeutic keratectomy in corneal diseases; few references have been found in our country to address the results of the application of the excimer laser in the elderly suffering from bullous keratopathy. Objective: To assess the results of the application of phototherapeutic keratectomy in the eldrly with bullous keratopathy. Methods: A case series study was conducted in 2009, in the Cornea Department of the Dr. Gustavo Aldereguía Lima General University Hospital of Cienfuegos. It included 16 elderly with bullous keratopathy not leading to penetrating keratoplasty. After considering the ethical and biomedical assessment, phototherapeutic keratectomy was performed. Treatment was indicated and follow up was performed with an assessment of symptoms and signs until the third month. Results: In the postoperative stage symptoms decreased and the most frequent sign was the haze followed by slight increase in best corrected visual acuity. Conclusions: The application of phototherapeutic keratectomy in patients that did not apply for cornea transplant helps improving eye condition, reduces symptoms and improves visual acuity.

  3. The results descemet-stripping automated endothelial keratoplasty at bullous keratopathy

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    S. V. Trufanov

    2014-07-01

    Full Text Available Purpose: to evaluate outcomes of the descemet-stripping automated endothelial keratoplasty using eye drops Kombinil-Duo dur- ing postoperative period.Methods: 26 patients suffered from bullous keratopathy underwent descemet-stripping automated endothelial keratoplasty using our own technique. We applied eye drops Kombinil-Duo during postoperative period.Results: 25 patients had clear corneas whole period of observation. Graft rejection occurred in 1 eye. Mean BSCVA was 0.47±0.21.Conclusion: Kombinil-Duo has been allowed to avoid infectious complication and to increase efficiency of the operative interven-tion during postoperative period.

  4. Superficial keratectomy and 360º conjunctival flap for bullous keratopathy in a dog: a case report

    Directory of Open Access Journals (Sweden)

    J.P.D. Ortiz

    2011-06-01

    Full Text Available A case of a two-year-old male Pinscher with a history of discomfort in the right eye was reported. The left eye had been enucleated by the referring veterinarian due to the same symptom with unsuccessful clinical treatment. The Schirmer tear test value was elevated and a decreased intraocular pressure was observed by applanation tonometry. Biomicroscopy revealed profuse corneal edema and keratoconus and fluorescein staining was negative. Gonioscopy and ophthalmoscopy did not provide any relevant data due to the corneal alterations. Bullous keratopathy was diagnosed. Surgery was performed in two steps: 1 superficial keratectomy and 360º conjunctival flap, and 2 superficial keratectomy to restore corneal transparency. Thirty days after the second superficial keratectomy, the third eyelid flap was removed. Conjunctivalization of the upper nasal quadrant of the cornea was observed. The axial portion of the cornea was transparent and vision was restored.

  5. Non-Descemet’s stripping automated endothelial keratoplasty for bullous keratopathy secondary to iridoschisis

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    Minezaki T

    2013-07-01

    Full Text Available Teruumi Minezaki, Takaaki Hattori, Hayate Nakagawa, Shigeto Kumakura, Hiroshi GotoDepartment of Ophthalmology, Tokyo Medical University, Shinjukuku, Tokyo, JapanPurpose: To report a case of bullous keratopathy secondary to iridoschisis treated by non-Descemet's stripping automated endothelial keratoplasty (nDSAEK.Case report: A 79-year-old woman was referred to our hospital with loss of vision in the left eye. Slit lamp examination of her left eye showed a shallow anterior chamber with cataract and schisis in the inferior quadrant of iris stroma. Bullous keratopathy secondary to iridoschisis was diagnosed. Cataract surgery with iridectomy succeeded to deepen the anterior chamber and remove the floating iris leaf, although corneal edema remained. Four days later, nDSAEK was performed, which resolved corneal edema and restored visual acuity.Conclusion: The two-step surgery of cataract surgery plus iridectomy followed by nDSAEK may be an effective strategy for treating bullous keratopathy secondary to iridoschisis.Keywords: iridoschisis, bullous keratopathy, non-Descemet's stripping automated endothelial keratoplasty

  6. The effects of riboflavin/ultraviolet: A corneal cross-linking on the signs and symptoms of bullous keratopathy

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    Hamid Gharaee

    2011-01-01

    Conclusion : The outcomes of this study indicate corneal cross-linking is not an effective treatment for bullous keratopathy with respect to VA and CCT, although it can improve irritation and discomfort.

  7. Recurrent rates and risk factors associated with recurrent painful bullous keratopathy after primary phototherapeutic keratectomy

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    Kasetsuwan N

    2015-09-01

    Full Text Available Ngamjit Kasetsuwan, Kanokorn Sakpisuttivanit, Usanee Reinprayoon, Vilavun Puangsricharern Department of Ophthalmology, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Bangkok, Thailand Objective: To assess the recurrent rate, mean survival time, and risk factors associated with recurrent painful bullous keratopathy (BK after primary treatment with phototherapeutic keratectomy.Methods: Medical records from 72 patients (72 eyes who had phototherapeutic keratectomy for painful BK were evaluated. Data for sex, age, duration of BK, associated ocular and systemic diseases (hypertension, diabetes mellitus, ischemic heart disease, asthma, dyslipidemia, and rheumatoid arthritis, frequency and degree of pain (grade 1–3, visual acuity, corneal thickness, intraocular pressure, and laser setting were extracted and analyzed.Results: The mean age of the patients was 64.2±11.4 years. The mean preoperative duration of BK was 15.0±11.0 months. Most patients had pseudophakic BK (69.40%. Majority of the cases had grade 3 degree of pain (48.60%. Glaucoma and hypertension were markedly found among these patients (51.40% and 19.40%, respectively. Preoperative mean intraocular pressure and corneal thickness were 13.70±4.95 mmHg and 734.1±83.80 µm, respectively. The mean laser diameter and depth were 8.36±1.22 mm and 38.89±8.81 µm, respectively. Systemic disease was significantly associated with the risk for developing recurrent painful BK (P=0.022, hazard ratio [HR] 1.673, 95% confidence interval [CI] 1.08–2.58. The overall recurrent rate was 51%. The average duration time of recurrent painful BK was 17.3±12.9 months (range 1–50 months. The median survival time before recurrence was 29.0±6.6 months.Conclusion: Systemic disease was found to be the only risk factor significantly associated with the development of recurrent painful BK. Low recurrent rate and long mean survival time showed that phototherapeutic

  8. Descemet Stripping Automated Endothelial Keratoplasty for Treatment of Pseudophakic Bullous Keratopathy

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    Banu Torun Acar

    2012-07-01

    Full Text Available Pur po se: To evaluate the results and complications of Descemet stripping automated endothelial keratoplasty (DSAEK in the treatment of pseudophakic bullous keratopathy (PBK. Ma te ri al and Met hod: Twenty eyes of 20 patients who underwent DSAEK with a diagnosis of PBK in our clinic between September 2010-January 2012 were included in this study. Pre- and postoperative best-corrected visual acuity (BCVA, refraction values, endothelial cell density, and graft position were analyzed. Complications and treatments were recorded. Re sults: The mean age of the 20 patients (11female, 9 male was 71.44±7.55 years (range: 56-83 years and the mean follow-up time was 11.35±3.90 months (range: 3-16 months. Preoperative mean BCVA was 0.04±0.044 and 12-month BCVA was 0.55±0.22 (p=0.001. Mean endothelial cell density was 1508.42±254.82 cell/mm² at 12-month follow-up visit. Dislocation of graft lenticule occurred in five patients and graft failure occurred in one patient. Dis cus si on: DSAEK, due to the more rapid rehabilitation, is an alternative to penetrating keratoplasty in the treatment of endothelial diseases despite the steep learning curve and different complications. (Turk J Ophthalmol 2012; 42: 263-68

  9. 软性角膜接触镜治疗大泡性角膜病变%Treatment of bullous keratopathy with soft contact lens

    Institute of Scientific and Technical Information of China (English)

    朱敏; 王诚忠; 沈念慈; 陈华洁

    2001-01-01

    Objective To assess the roles of the soft contact lens(SCL) in the treatment of bullous keratopathy.Methods Seventeen cases with bullous keratopathy wore SCL continuously and disposed it each month. All cases were followed up to observe whether the clinical symptoms were relieved and complications appear.Results  Seventeen cases were followed up for 2 to 22 months. The results suggested that the clinical symptoms were relieved significantly without complications.Conclusion SCL can relieve the clinical symptoms and improve the life quality of patients with bullous keratopathy significantly.%目的评价软性角膜接触镜(soft contact lens, SCL)在大泡性角膜病变治疗中的作用。方法 17例大泡性角膜病变患者配戴SCL,每月更换1次,随访观察患者临床症状是否改善及有无并发症出现。结果 17例患者随访2~22mo,临床症状都得到一定改善,无明显并发症出现。结论SCL能明显改善大泡性角膜病变患者的临床症状,提高患者的生活质量。

  10. 兔大泡性角膜病变疼痛状态客观评价的方法学研究%A pilot study on evaluation of pain and distress status of bullous keratopathy rabbit model

    Institute of Scientific and Technical Information of China (English)

    荣蓓; 白静; 晏晓明

    2013-01-01

    Background Identifying and testing of pain is very necessary for the care and decrease of the suffering of experimental animal in medical experiment.Effective method for testing the pain and distress status of experimental animal with eye disease is still absent in China.Objective This pilot study was to establish an evaluating system for assessing the pain and distress status of bullous keratopathy rabbit model.Methods This study was approved by the Ethic Committee of Beijing University First Hospital.Twelve healthy New Zealand rabbits were selected in this experiment.Bullous keratopathy model was established in the left eyes of 9 rabbits by scraping corneal endothelium as the experimental team,and other 3 rabbits were served as the control team.The cornea lesion was examined by manipulate slit lamp,and the central cornea thickness (CCT) was measured by ultrasound biomicroscopy (UBM).Weight+20 Indexes For Pain and Distress Status Referring Guidelines for Pain and Distress in Laboratory Animals made by International Animal Care and Use Committee (IACUC) were assessed and measured as well.Results Corneal edema and opacity were obvious 1 day after surgery.Corneal bullous appeared from the third day after surgery,and cornea erosion was seen at the location of bullous breakage.The corneal lesions remained until 14 days after surgery.CCT value was (1468±100),(2313±588),(2391±271) and (2362±151) μm,respectively in day 1,3,7 and 14 after the establishment of models,which showed significant increase in comparison with the preoperative CCT (390±6)μm (all P=0.000).However,no significant difference was seen in the CCT between day 3,7 and 14 (P>0.05).Body weight of the rabbits was (3.29±0.20),(3.20-0.17),(2.77±0.25) and (3.10±0.30)kg respectively in day 1,3,7 and 14 after operation,with significant decrease in comparison with the pre-operative weight (3.52-0.18)kg in the experimental team (P=0.008,0.007,0.003,0.004).The scores for pain and distress status of all

  11. Decorin and biglycan of normal and pathologic human corneas

    Science.gov (United States)

    Funderburgh, J. L.; Hevelone, N. D.; Roth, M. R.; Funderburgh, M. L.; Rodrigues, M. R.; Nirankari, V. S.; Conrad, G. W.

    1998-01-01

    PURPOSE: Corneas with scars and certain chronic pathologic conditions contain highly sulfated dermatan sulfate, but little is known of the core proteins that carry these atypical glycosaminoglycans. In this study the proteoglycan proteins attached to dermatan sulfate in normal and pathologic human corneas were examined to identify primary genes involved in the pathobiology of corneal scarring. METHODS: Proteoglycans from human corneas with chronic edema, bullous keratopathy, and keratoconus and from normal corneas were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), quantitative immunoblotting, and immunohistology with peptide antibodies to decorin and biglycan. RESULTS: Proteoglycans from pathologic corneas exhibit increased size heterogeneity and binding of the cationic dye alcian blue compared with those in normal corneas. Decorin and biglycan extracted from normal and diseased corneas exhibited similar molecular size distribution patterns. In approximately half of the pathologic corneas, the level of biglycan was elevated an average of seven times above normal, and decorin was elevated approximately three times above normal. The increases were associated with highly charged molecular forms of decorin and biglycan, indicating modification of the proteins with dermatan sulfate chains of increased sulfation. Immunostaining of corneal sections showed an abnormal stromal localization of biglycan in pathologic corneas. CONCLUSIONS: The increased dermatan sulfate associated with chronic corneal pathologic conditions results from stromal accumulation of decorin and particularly of biglycan in the affected corneas. These proteins bear dermatan sulfate chains with increased sulfation compared with normal stromal proteoglycans.

  12. Quantitative & qualitative analysis of endothelial cells of donor cornea before & after penetrating keratoplasty in different pathological conditions

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    Aruna K.R. Gupta

    2016-01-01

    Full Text Available Background & objectives: Endothelial cells of the donor cornea are known to be affected quantitatively and qualitatively in different pathological conditions after penetrating keratoplasty (PK and this has direct effect on the clarity of vision obtained after PK. This study was undertaken to analyze the qualitative and quantitative changes in donor endothelial cells before and after PK in different pathological conditions. Methods: A prospective investigational analysis of 100 consecutive donor corneas used for penetrating keratoplasty between June 2006 and June 2008, was conducted. The patients were evaluated on the first day, at the end of first week, first month, third and six months and one year. Results: A decrease was observed in endothelial cell count in all pathological conditions. After one year of follow up the loss was 33.1 per cent in corneal opacity, 45.9 per cent in acute infective keratitis (AIK, 58.5 per cent in regrafts, 28.5 per cent in pseudophakic bullous keratopathy (PBK, 37 per cent in descemetocele, 27 per cent in keratoconus and 35.5 per cent in aphakic bullous keratopathy (ABK cases. Interpretation & conclusions: The endothelial cell loss was highest in regraft cases which was significant (P<0.05, while the least endothelial cell loss was seen in keratoconus cases. The cell loss was associated with increase in coefficient of variation (CV, i.e. polymegathism and pleomorphism. Inspite of this polymegathism and pleomorphism, the clarity of the graft was maintained.

  13. Quantitative & qualitative analysis of endothelial cells of donor cornea before & after penetrating keratoplasty in different pathological conditions

    Science.gov (United States)

    Gupta, Aruna K.R.; Gupta, Roopam K.R.

    2016-01-01

    Background & objectives: Endothelial cells of the donor cornea are known to be affected quantitatively and qualitatively in different pathological conditions after penetrating keratoplasty (PK) and this has direct effect on the clarity of vision obtained after PK. This study was undertaken to analyze the qualitative and quantitative changes in donor endothelial cells before and after PK in different pathological conditions. Methods: A prospective investigational analysis of 100 consecutive donor corneas used for penetrating keratoplasty between June 2006 and June 2008, was conducted. The patients were evaluated on the first day, at the end of first week, first month, third and six months and one year. Results: A decrease was observed in endothelial cell count in all pathological conditions. After one year of follow up the loss was 33.1 per cent in corneal opacity, 45.9 per cent in acute infective keratitis (AIK), 58.5 per cent in regrafts, 28.5 per cent in pseudophakic bullous keratopathy (PBK), 37 per cent in descemetocele, 27 per cent in keratoconus and 35.5 per cent in aphakic bullous keratopathy (ABK) cases. Interpretation & conclusions: The endothelial cell loss was highest in regraft cases which was significant (P<0.05), while the least endothelial cell loss was seen in keratoconus cases. The cell loss was associated with increase in coefficient of variation (CV), i.e. polymegathism and pleomorphism. Inspite of this polymegathism and pleomorphism, the clarity of the graft was maintained. PMID:27121519

  14. A specific analysis of morphology and thickness of cornea in early-stage congenital aniridic keratopathy%先天性无虹膜患者早期角膜形态及厚度特点

    Institute of Scientific and Technical Information of China (English)

    邵毅; 王乐; 余瑶; 张广斌; 裴重刚; 邹文进; 高桂平; 毛益辉; 李凯军

    2013-01-01

    Objective To search for the characteristics of early congenital aniridic keratopathy (CAK) in corneal topography and thickness.Methods Twelve patients (24 eyes) with early CAK and 12 volunteers (24 eyes) were recruited in this clinical prospective and comparative series study.Instruments applied included the Orbscan IIZ Corneal Topography.Results In this study,there was no statistically significance between the two groups in ages,gender and axis length (P >0.05).Flat cornea (less than 41.5D) ratio and topography of the oval,irregular bow-shaped and irregular-shaped were increased significantly in patients with early CAK.There were statistically significance in the thinnest thickness of cornea,mean A,mean K,sim K,corneal thickness index (CTI),3.0mm Zone Irreg and 5.0mm Zone Irreg between the early CAK and control (P <0.05).A significant increase of pachymetry appeared in the early CAK group compared with the control group.There were no statistically significance in mean A and 3.0mm Zone Irreg in both groups (P >0.05).Conclusions The characteristic of early CAK in corneal topography is that corneal axial refractive power descend and corneal thickness decrease.%目的 先天性无虹膜(congenital aniridic keratopathy,CAK)是一种常见的常染色体显性遗传性眼病,文中研究通过对比观察CAK早期患者的角膜地形图,以了解此疾病角膜的形态及厚度变化特点.方法 采用前瞻性病例对照研究方法.对12名(24只眼)健康志愿者及12例(24只眼)临床确诊为CAK早期患者进行角膜地形图检查,并进行对比分析,了解CAK早期患者角膜地形图的参数改变特点.结果 两组在年龄、性别及眼轴长度上的差异无统计学意义(P值均>0.05).在角膜前表面屈光力图形态上,CAK早期患者椭圆形、不规则蝴蝶结形和不规则形地形图的比例以及扁平角膜比例均明显升高.角膜地形图参数中,角膜最薄点厚度、角膜3 mm区屈率(mean K

  15. Bullous mastocytosis

    OpenAIRE

    Nayak Surajit; Acharjya Basanti; Devi Basanti; Behera Samira

    2007-01-01

    Mastocytosis is a condition characterized by the disorderly infiltration of mast cells in several tissues and comprises many different clinical situations varying from indolent cutaneous forms to malignant and systemic conditions. Bullous mastocytosis is a rare variant of cutaneous mastocytosis manifested by the diffuse infiltration of skin by mast cells, where cutaneous bullae is the predominant feature. We present this case for its rarity and diversity.

  16. Bullous mastocytosis

    Directory of Open Access Journals (Sweden)

    Nayak Surajit

    2007-01-01

    Full Text Available Mastocytosis is a condition characterized by the disorderly infiltration of mast cells in several tissues and comprises many different clinical situations varying from indolent cutaneous forms to malignant and systemic conditions. Bullous mastocytosis is a rare variant of cutaneous mastocytosis manifested by the diffuse infiltration of skin by mast cells, where cutaneous bullae is the predominant feature. We present this case for its rarity and diversity.

  17. Bullous Scabies.

    Science.gov (United States)

    Luo, Di-Qing; Huang, Mei-Xing; Liu, Juan-Hua; Tang, Wen; Zhao, Yu-Kun; Sarkar, Rashmi

    2016-09-01

    Scabies is a common contagious cutaneous disease and usually affects the young, characterized by polymorphous lesions that may present as burrows, pruritic papules, and inflammatory nodules. Bullous scabies (BS) is its rather rare subtype, mimicking bullous pemphigoid. We report a 15-year-old Chinese boy presenting with 1-month history of pruritic bullae on his penile skin, showing poor response to both topical steroids and systemic antihistamines, but cured by sulfur ointment alone. No recurrence occurred in the 5 years of follow-up. We also reviewed the published cases. Up to date, 44 cases, including the present, have been reported. Of them, 30 were male and 14 were female. The age range was from 1 to 89 years old, with a median age of 70.6 years. The bullous lesions may involve the arms, legs, trunk, genitals, feet, buttocks, thighs, neck, inguinal folds, and may even be generalized. Trunk and extremities are the most common involved locations. Facial or mucosa involvement had never been reported. The histological findings present as a subepidermal split with variable inflammatory infiltrate predominantly neutrophils, and eosinophilic spongiosis, or both. Eighteen of 32 patients showed positive deposition of linear-granular IgG or complement 3 alone or in various combinations, and five of 24 patients revealed circulating IgG. All the 40 cases with therapeutic details were cured by antiscabietic remedy. BS always involves the trunk and extremities. It has a predilection for elderlies and males. The treatments for BS are similar to those of classical scabies. PMID:27402514

  18. Perfil do paciente com ceratopatia bolhosa pós-facectomia atendidos em hospital público Profile of patient with aphakic/pseudopfakic bullous keratopaty attended at public hospital

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    Marcony Rodrigues de Santhiago

    2009-08-01

    Full Text Available Objetivo: Identificar e analisar criticamente o perfil dos pacientes com ceratopatia bolhosa atendidos em um hospital municipal. Métodos: Foi realizada análise retrospectiva de 35 olhos de 35 pacientes em acompanhamento no setor de córnea e doenças externas do Hospital da Piedade, que apresentavam ceratopatia bolhosa pós-facectomia. Foram excluídos aqueles onde não se conseguiu determinar características pré e per-operatórias fundamentais em nossa pesquisa. Esta seleção de pacientes foi realizada durante o período de janeiro de 2007 a junho de 2007. Resultados: Como principais resultadosobservou-se que a ceratopatia bolhosa ocorreu em 22.86 % (8 olhos dos casos pós-facectomia extracapsular (FEC e 74.28 % (26 olhos pós- facoemulsificação. Em 25.71 % (9 olhos foi documentado rotura de cápsula posterior (RCP com perda vítrea. Em 94.29 % (33 olhos a lente intraocular se localizava na câmara posterior, e dois pacientes estavam afácicos. A acuidade visual de todos os pacientes era pior que 20/200. O tempo médio de evolução entre a facectomia e o diagnostico de ceratopatia bolhosa foi 11,93 meses (1 - 44 meses. Conclusão: A análise adequada do endotélio corneano é condição essencial para o sucesso da cirurgia de catarata com transparência corneal, e que a ceratopatia bolhosa parece estar mais associada à facoemulsificação. Esta informação deve ser interpretada com cautela, já que esta relação pode vir do maior numero de facoemulsificações realizadas no Hospital onde foi realizado o estudo e muitas destas por cirurgiões em treinamento.Objective: Analyze and identify critically the profile of patients with aphakic/pseudophakic bullous keratopathy attended at a public hospital. Methods: Retrospective analysis of 35 eyes of 35 patients with diagnosis of bullous keratopathy, which has been followed in the department of cornea and external diseases of Hospital da Piedade. The one whose pre and per operative data we

  19. Effect of Stem Cell Therapy on Induced Diabetic Keratopathy in Albino Rat

    Science.gov (United States)

    Zickri, Maha Baligh; Ahmad, Nagwa Abdel Wahab; Maadawi, Zeinab Mohamad El; Mohamady, Yasmin Kamal; Metwally, Hala Gabr

    2012-01-01

    Background and Objectives: Type 2 diabetes mellitus (DM) is a prevalent disorder. Diabetic keratopathy is a well-known ocular complication secondary to type 2 DM. Topical insulin application did not affect apoptosis and necrosis levels in corneal epithelium. Autologous cell transplantation is not a viable option for diabetic patients with bilateral limbal stem cell deficiency. The present study aimed at assessing the possible effect of hemopoeitic stem cell (HSC) therapy on induced diabetic keratopathy in albino rat. Methods and Results: Fifteen male albino rats were divided into control group of 2 rats, diabetic group of 8 rats receiving single intraperitoneal (IP) injection of 50 mg/kg streptozotocin (STZ). 3 animals were sacrificed 6 weeks following confirmation of diabetes to confirm keratopathy and 5 rats were sacrificed 4 weeks following confirmation of keratopathy. SC therapy group included 5 rats injected with HSCs 6 weeks following confirmation of diabetes and sacrificed 4 weeks following SC therapy. Cord blood collection, stem cells isolation and labeling were performed. Eye specimens were subjected to histological, histochemical, immunohistochemical, morphometric and statistical studies. In diabetic group, the central cornea showed multiple cells with vacuolated cytoplasm and dark nuclei, focal epithelial discontinuity, reduced corneal thickness and less number of layers of corneal and conjunctival epithelia. In stem cell therapy group, few cells with vacuolated cytoplasm and dark nuclei were found in the corneal and conjunctival epithelia with more number of epithelial layers. Conclusions: A definite ameliorating effect of HSC therapy was detected on diabetic keratopathy. The therapeutic cells were effective in limiting corneal epithelial changes. PMID:24298355

  20. Energy dispersive x-ray analysis of the cornea. Application to paraffin sections of normal and diseased corneas

    International Nuclear Information System (INIS)

    The distribution of chemical elements in the normal human cornea was studied by energy dispersive x-ray analysis and scanning electron microscopy of routinely prepared paraffin sections. Calcium, phosphorus, and sulfur were consistently present in quantities above background and varied in concentration regionally. Analysis of fresh-frozen tissue, an approximation of the in vivo state, gave a similar elemental profile to paraffin sections, except for the loss of diffusable electrolytes in the latter. After fixation, S was the most abundant element and was highest in Descemet's membrane. Corneas with granular, lattice, macular, and Fuchs endothelial dystrophies, band keratopathy, and spheroidal degeneration were also examined. Characteristic patterns of abnormal S and Ca distribution were found in each of the dystrophies. The relative proportions of Ca, P, and S gave diagnostic profiles for distinguishing band keratopathy and spheroidal degeneration

  1. Regression of Labrador keratopathy following cataract extraction.

    OpenAIRE

    Dahan, E; Judelson, J; Welsh, N H

    1986-01-01

    Labrador keratopathy (LK) is an acquired corneal degeneration thought to be caused by chronic exposure to solar irradiation. Reports so far suggest that it is a progressive or at least a stationary condition. There are no detailed reports on recommended therapy. A prospective clinical study was conducted to show regression of LK following extracapsular cataract extraction. Seventeen black patients (26 eyes) with LK and mature cataracts underwent extracapsular cataract extraction. The severity...

  2. Marijuana abuse and bullous emphysema

    OpenAIRE

    Harsh Golwala

    2012-01-01

    Marijuana abuse has been on an increasing trend worldwide in the last decade. We hereby report a case of severe bullous emphysema resulting in pneumothorax in a patient with few years of marijuana abuse. We also review the major conditions in the differential diagnosis of bullous emphysema in adults.

  3. Infectious crystalline keratopathy associated with Klebsiella oxytoca

    OpenAIRE

    Chou, Timothy Y; Adyanthaya, Rohit

    2012-01-01

    Purpose The purpose of this study is to report a novel case of a Klebsiella oxytoca-associated infectious crystalline keratopathy Methods This is a case report study. Results An 80-year-old woman presented with complaint of noticing a white spot in the left eye for 2 to 3 days, as well as mild soreness and discharge. Past ocular history was notable for a failed left corneal transplant for which she was taking prednisolone acetate 1 % twice per day. On slit-lamp examination, there was an exten...

  4. Superficial keratectomy and 360º conjunctival flap for bullous keratopathy in a dog: a case report

    OpenAIRE

    J.P.D. Ortiz; C.B.S. Lisbão; F.L.C. Brito; Martins, B.; J.L. Laus

    2011-01-01

    A case of a two-year-old male Pinscher with a history of discomfort in the right eye was reported. The left eye had been enucleated by the referring veterinarian due to the same symptom with unsuccessful clinical treatment. The Schirmer tear test value was elevated and a decreased intraocular pressure was observed by applanation tonometry. Biomicroscopy revealed profuse corneal edema and keratoconus and fluorescein staining was negative. Gonioscopy and ophthalmoscopy did not provide any relev...

  5. Nutrition and bullous skin diseases.

    Science.gov (United States)

    Fedeles, Flavia; Murphy, Michael; Rothe, Marti J; Grant-Kels, Jane M

    2010-01-01

    Autoimmune and nonautoimmune bullous diseases can both be associated with significant morbidity and mortality. Although our understanding of the pathogenic mechanisms of these diseases has increased tremendously, there is still much to learn about the various factors affecting their onset, course, and therapy. In recent years, increasing information has been published about the effect of vitamins, minerals, and other nutrients on bullous skin diseases. Some factors are believed to be inducers (thiol and phenol-containing foods in pemphigus), whereas others are believed to be protective (antioxidants in cutaneous porphyrias). This contribution reviews the evidence in the literature of the role of various dietary factors in bullous diseases, including the nonautoimmune and the deficiency dermatoses. Additional studies and new investigations are needed to provide a better understanding of the specific associations of dietary factors with bullous diseases and better management for patients affected by these conditions. PMID:21034987

  6. Bullous pemphigoid after radiation therapy

    International Nuclear Information System (INIS)

    Electron beam therapy applied to a lymph node metastasis from a squamous cell carcinoma was followed by the development of histologically and immunologically typical bullous pemphigoid, the lesions being initially strictly confined to the irradiation area. This observation suggests that the bullous pemphigoid antigen may be altered or unmasked by electron beam radiotherapy, leading subsequently to the production of autoantibodies. The disease in this case effectively responded to the administration of tetracycline and niacinamide, a therapeutic regimen described recently

  7. Tibolone induced Bullous pemphigoid.

    Directory of Open Access Journals (Sweden)

    Vishal. R. Tandon, Annil Mahajan* & Sudhaa Sharma**

    2008-01-01

    Full Text Available We present first ever report of Bullous pemphigoid induced by Tibolone, a STEAR (Selective tissueestrogenic activity regulator that has progestogenic, some androgenic as well as estrogenic effects prescribedas an alternative to estrogen replacement therapy for treatment of climacteric symptoms in one ofthe 51 year old postmenopausal women with one and half year duration since menopause with previoushistory of use of estrogen progesterone pills during her active sexual life. The mechanism for this ADR isnot well understood. But possible explanation could be progesterone activity of the drug leading to autoimmunityas reported previously. The present patient was managed by dechallenge of drug, local, oral corticosteroidsand injectable, methotrexate, along with folic acid and antibiotic coverage fearing anemia andsecondary infections.

  8. Superficial Punctate Keratitis

    Science.gov (United States)

    ... an allergy to eye drops, the drug or eye drops must be discontinued. Resources In This Article Figure 1 Locating the Cornea Corneal Disorders Introduction to Corneal Disorders Bullous Keratopathy Corneal Transplantation Corneal Ulcer Herpes Simplex Keratitis Herpes Zoster Ophthalmicus ...

  9. Keratopathy due to ophthalmic drug abuse with corneal melting and perforation presenting as Mooren-like ulcer: A case report

    Science.gov (United States)

    WU, HUI; HU, YUAN; SHI, XIAO-RU; XU, FEIHONG; JIANG, CHUN-YING; HUANG, RONG; JIA, HUI

    2016-01-01

    The present report describes the clinical course and treatment of a Mooren-like ulcer associated with abuse of topical anesthetics and dexamethasone. A 38-year-old male physician treated himself with lidocaine, tetracaine and dexamethasone (DEX) eye drops for severe pain and decline of vision in both eyes. After six months of treatment, his right cornea exhibited annular melting with full-thickness stromal infiltration at the limbus and central corneal haze. His left cornea was completely melted and exhibited iris prolapse. The patient was treated with topical antibiotics, lubricants and underwent a binocular keratoplasty. The surgery was successful and after eight months the postoperative best-corrected visual acuities were counting fingers in the right eye and light perception in the left eye. In summary, this report documents a severe case of keratopathy presenting as Mooren-like ulcer caused by topical anesthetics and DEX, which were treated via keratoplasty, resulting in useful vision being retained.

  10. Diabetic and non-diabetic human cornea and tear γ-glutamyl transpeptidase activity

    OpenAIRE

    Burnham, Jordan M; Sakhalkar, Monali; Langford, Marlyn P; Liang, Chanping; Redens, Thomas B.; Jain, Sushil K.

    2013-01-01

    Background Diabetes-related eye disease is due in part to oxidative stress. Gamma-glutamyl transpeptidase (GGT) is a γ-glutamyl cycle enzyme that protects against oxidative stress via glutathione recapture. This study investigates corneal and Schirmer tears GGT activity in diabetic and non-diabetic adults aged 50 to 83 years old. Methods GGT activity was determined by colorimetric assay on 50 corneas from 14 diabetic (without keratopathy) and 20 non-diabetic donors and on Schirmer type 1 test...

  11. Corneal endothelial changes in superficial epithelial keratopathy.

    Science.gov (United States)

    Brooks, A M; Grant, G; Gillies, W E

    1986-05-01

    A series of five cases is described in which superficial punctate keratopathy was associated with endothelial cell changes. The most striking change was the presence of dark areas or blebs, usually two to four cell diameters in extent. There was also distortion and crumpling of the corneal endothelium, mild pleomorphism and polymegathism of the endothelial cells, with a reduced cell count in some cases. These blebs have previously been reported in hard and soft contact lens wearers and are due to intercellular oedema with separation of endothelial cells from Descemet's membrane. Anoxia and interference with osmosis have been proposed as possible mechanisms for the production of these blebs, but our cases demonstrate that fine disruption of the corneal epithelium can affect the integrity of the corneal endothelium and may lead to significant damage over a long period of time. PMID:3801208

  12. Enucleaton of the right eye due to large choroidal melanoma with simultaneous penetrating cornea transplantation from OD to OS (Case report.

    Directory of Open Access Journals (Sweden)

    E. A. Korchuganova

    2013-01-01

    Full Text Available There is presentation case report of 75‑year old woman with choroidal melanoma (T3N0M0 on the right eye and failed graft on the left pseudophakic eye with far advanced glaucoma and ARMD. No treatment was given to the leading eye with VA 0,2. VA of OS = 1 / ∞ pr.l.certa; PKP OS in 2008 for pseudophakic bullous keratopathy on the eye with far advanced glaucoma. IOP was normal after previous filtering surgery. After PKP VA = 0,04; clear graft during 2 years; then gradually opacification and vascularization occurred. VA dropped to light perception. Echography OD — tumor h 8,29 mm, d 21,77 mm. No ingrowth of tumor into anterior segment of the eye; VA OD = 0 (no light perception. Concerning the need to enucleate the right eye with large choroidal melanoma, the advantage of using corneal autograft from OD to OS, location of tumor in the posterior pole with no ingrowth in anterior segment, the decision was made to perform the following operation — to enucleate the right eye and transplant simultaneously corneal graft from OD on OS. Patient was discharged from the Ophthalmology Hospital with VA OS = 0,01, during next week VA improved to 0,02. 8,0 mm graft isclear, fixed with 8 interruptured and continious suture 10 / 0‑nylon. Anterior chamber — normal depth, atrophic iris, stable position of PC IOL. Optic nerve head is pale with subtotal deep glaucomatous excavation. Conclusion: presented case report demonstrates the rarepossibility to use cornea after enucleation the eye with large malignant tumor (located in the posterior pole for grafting in the only eye with failed vascularised graft. It was the only possibility for this patient to restore some vision.

  13. Enucleaton of the right eye due to large choroidal melanoma with simultaneous penetrating cornea transplantation from OD to OS (Case report.

    Directory of Open Access Journals (Sweden)

    E. A. Korchuganova

    2014-07-01

    Full Text Available There is presentation case report of 75‑year old woman with choroidal melanoma (T3N0M0 on the right eye and failed graft on the left pseudophakic eye with far advanced glaucoma and ARMD. No treatment was given to the leading eye with VA 0,2. VA of OS = 1 / ∞ pr.l.certa; PKP OS in 2008 for pseudophakic bullous keratopathy on the eye with far advanced glaucoma. IOP was normal after previous filtering surgery. After PKP VA = 0,04; clear graft during 2 years; then gradually opacification and vascularization occurred. VA dropped to light perception. Echography OD — tumor h 8,29 mm, d 21,77 mm. No ingrowth of tumor into anterior segment of the eye; VA OD = 0 (no light perception. Concerning the need to enucleate the right eye with large choroidal melanoma, the advantage of using corneal autograft from OD to OS, location of tumor in the posterior pole with no ingrowth in anterior segment, the decision was made to perform the following operation — to enucleate the right eye and transplant simultaneously corneal graft from OD on OS. Patient was discharged from the Ophthalmology Hospital with VA OS = 0,01, during next week VA improved to 0,02. 8,0 mm graft isclear, fixed with 8 interruptured and continious suture 10 / 0‑nylon. Anterior chamber — normal depth, atrophic iris, stable position of PC IOL. Optic nerve head is pale with subtotal deep glaucomatous excavation. Conclusion: presented case report demonstrates the rarepossibility to use cornea after enucleation the eye with large malignant tumor (located in the posterior pole for grafting in the only eye with failed vascularised graft. It was the only possibility for this patient to restore some vision.

  14. [Bullous autoimmune disorders in children].

    Science.gov (United States)

    Sárdy, M; Kasperkiewicz, M

    2013-06-01

    We review the pathogenesis, clinical features, diagnosis, differential diagnosis, and therapy of autoimmune bullous skin diseases of childhood, especially of the most common linear IgA dermatosis. In autoimmune bullous diseases, autoantibodies are formed against different adhesion molecules of the skin. These are not only pathophysiologically relevant, but also serve as basis for diagnosis and follow-up of these diseases. In case an autoimmune bullous disease is suspected, histopathology and immunohistopathology (direct immunofluorescence microscopy) as well as serological tests (indirect immunofluorescence microscopy, ELISA, immunoblot) should be performed. Therapy depends on the diagnosis. In IgA-mediated pathogenesis, dapsone can be successfully used. In IgG-mediated diseases, immunosuppression with corticosteroids and steroid-sparing agents should be initiated, although only local therapy is sufficient to control a self-limiting pemphigus neonatorum. In dermatitis herpetiformis, a life-long gluten-free diet is recommended. PMID:23677541

  15. Linear IgA Bullous Dermatosis

    DEFF Research Database (Denmark)

    Lings, Kristina; Bygum, Anette

    2015-01-01

    Linear IgA bullous dermatosis (LAD) is an autoimmune, chronic bullous disease affecting primarily young children and adults. Studies on LAD are relatively sparse and from Scandinavia we could only find a few case reports. Therefore we decided to conduct a retrospective investigation of patients...

  16. Cloudy corneas as an initial presentation of multiple myeloma

    Directory of Open Access Journals (Sweden)

    Sharma P

    2014-04-01

    Full Text Available Priyanka Sharma,1 Haifa A Madi,1 Richard Bonshek,2 Stephen J Morgan11Sunderland Eye Infirmary, Sunderland, UK; 2National Specialist Ophthalmic Pathology Service, Department of Histopathology, Manchester Royal Infirmary, Manchester, UKSummary: We report a case of previously unsuspected myeloma, presenting with cornea verticillata due to intracorneal paraprotein deposition.History: An 85-year-old female presented via her optician with a 4-month history of cloudy vision. She had undergone an uneventful bilateral phacoemulsification surgery 7 years earlier. Extensive spiraling corneal epithelial opacification was noted on slit-lamp examination. On further investigation, she was found to have a previously unsuspected low-grade multiple myeloma. We established the nature of the corneal deposits with corneal epithelial biopsy histopathology and electron microscopy. It is very rare for multiple myeloma to present in this fashion. Ophthalmologists should be aware that such a presentation may rarely be due to systemic multiple myeloma.Keywords: corneal crystals, multiple myeloma, crystalline keratopathy, vortex keratopathy

  17. Molecular biological aspects of acquired bullous diseases

    DEFF Research Database (Denmark)

    Dabelsteen, Erik

    1998-01-01

    genes have been cloned. The antigens which react with autoantibodies from patients with bullous pemphigoid, cicatricial pemphigoid, acquired epidermolysis bullosa, and linear IgA disease are all proteins of the hemidesmosome basement membrane complex. Interestingly, most of the antigens also appear to...... be the target for mutations seen in patients with the inherited type of epidermolysis bullosa in which bullous lesions are a prominent clinical feature....

  18. Treatment of Subepidermal Autoimmune Bullous Diseases

    OpenAIRE

    Ekin Şavk

    2011-01-01

    The common therapeutic goal for all subepidermal bullous diseases is to prevent inflammation and production of pathogenic autoantibodies and/or to facilitate the elimination of these antibodies. Diseases included in this group are the pemphigoids, linear IgA dermatosis, epidermolysis bullosa acquisita, dermatitis herpetiformis and bullous systemic erythematosus. Corticosteroids are the most commonly used systemic medication. Other alternatives include dapsone, various antibiotics and nicotina...

  19. Azithromycin induced bullous fixed drug eruption

    Science.gov (United States)

    Das, Anupam; Sancheti, Karan; Podder, Indrashis; Das, Nilay Kanti

    2016-01-01

    Fixed drug eruption (FDE) is a common type of drug eruption seen in skin clinics. It is characterized by solitary or multiple, round to oval erythematous patches with dusky red centers, some of which may progress to bulla formation. Bullous FDE may be caused by a number of drugs. We hereby describe a case of azithromycin-induced bullous FDE; to the best of our knowledge, this is the first such case being reported. PMID:26997729

  20. Photodynamic therapy (ALA-PDT) in the treatment of pathological states of the cornea

    Science.gov (United States)

    Switka-Wieclawska, Iwona; Kecik, Tadeusz; Kwasny, Miroslaw; Graczyk, Alfreda

    2003-10-01

    Each year an increasing amount of research is published on the use of photodynamic therapy in medicine. The most recent research has focused mostly on the use of photosensitizer called vertoporphyrin (Visudyne) is the treatment of subretinal neovascularization in age-related macular degeneration (AMD) or myopia, following a substantial amount of ophthalmology research mostly experimental on the application of the method in diagnosis and treatment of some eye tumors. In the Department of Ophthalmology of Polish Medical University in Warsaw, PDT was used as supplementary method in a selected group of patients with chronic virus ulcer of the cornea and keratopathies. During the treatment 5-aminolevulinic acid (5-ALA) was applied in ointment form as a photosensitizer activated with light wave of 633 nm. It appears, on the basis of the results obtained, that photodynamic therapy (ALA-PDT) may become in the future a valuable supplement to the methods being used at the present treating pathological states of the cornea.

  1. Bullous Systemic Lupus Erythematosus: Case report

    Science.gov (United States)

    Miziara, Ivan Dieb; Mahmoud, Ali; Chagury, Azis Arruda; Alves, Ricardo Dourado

    2013-01-01

    Summary Introduction: Bullous systemic lupus erythematosus (BSLE) is an autoantibody-mediated disease with subepidermal blisters. It is a rare form of presentation of SLE that occurs in less than 5% of cases of lupus. Case Report: A 27-year-old, female, FRS patient reported the appearance of painful bullous lesions in the left nasal wing and left buccal mucosa that displayed sudden and rapid growth. She sought advice from emergency dermatology staff 15 days after onset and was hospitalized with suspected bullous disease. Intravenous antibiotics and steroids were administered initially, but the patient showed no improvement during hospitalization. She displayed further extensive injuries to the trunk, axillae, and vulva as well as disruption of the bullous lesions, which remained as hyperemic scars. Incisional biopsy of a lesion in the left buccal mucosa was performed, and pathological results indicated mucositis with extensive erosion and the presence of a predominantly neutrophilic infiltrate with degeneration of basal cells and apoptotic keratinocytes. Under direct immunofluorescence, the skin showed anti-IgA, anti-IgM, and anti-IgG linear fluorescence on the continuous dermal side of the cleavage. Indirect immunofluorescence of the skin showed conjugated anti-IgA, was anti-IgM negative, and displayed pemphigus in conjunction with anti-IgG fluorescence in the nucleus of keratinocytes, consistent with a diagnosis of bullous lupus erythematosus. Discussion: BSLE is an acquired autoimmune bullous disease caused by autoantibodies against type VII collagen or other components of the junctional zone, epidermis, and dermis. It must be differentiated from the secondary bubbles and vacuolar degeneration of the basement membrane that may occur in acute and subacute cutaneous lupus erythematosus. PMID:25992032

  2. Bullous Systemic Lupus Erythematosus: Case report

    Directory of Open Access Journals (Sweden)

    Miziara, Ivan Dieb

    2014-01-01

    Full Text Available Introduction: Bullous systemic lupus erythematosus (BSLE is an autoantibody-mediated disease with subepidermal blisters. It is a rare form of presentation of SLE that occurs in less than 5% of cases of lupus. Case Report: A 27-year-old, female, FRS patient reported the appearance of painful bullous lesions in the left nasal wing and left buccal mucosa that displayed sudden and rapid growth. She sought advice from emergency dermatology staff 15 days after onset and was hospitalized with suspected bullous disease. Intravenous antibiotics and steroids were administered initially, but the patient showed no improvement during hospitalization. She displayed further extensive injuries to the trunk, axillae, and vulva as well as disruption of the bullous lesions, which remained as hyperemic scars. Incisional biopsy of a lesion in the left buccal mucosa was performed, and pathological results indicated mucositis with extensive erosion and the presence of a predominantly neutrophilic infiltrate with degeneration of basal cells and apoptotic keratinocytes. Under direct immunofluorescence, the skin showed anti-IgA, anti-IgM, and anti-IgG linear fluorescence on the continuous dermal side of the cleavage. Indirect immunofluorescence of the skin showed conjugated anti-IgA, was anti-IgM negative, and displayed pemphigus in conjunction with anti-IgG fluorescence in the nucleus of keratinocytes, consistent with a diagnosis of bullous lupus erythematosus. Discussion: BSLE is an acquired autoimmune bullous disease caused by autoantibodies against type VII collagen or other components of the junctional zone, epidermis, and dermis. It must be differentiated from the secondary bubbles and vacuolar degeneration of the basement membrane that may occur in acute and subacute cutaneous lupus erythematosus.

  3. Bullous Systemic Lupus Erythematosus: Case report

    OpenAIRE

    Miziara, Ivan Dieb; Mahmoud, Ali; Chagury, Azis Arruda; Alves, Ricardo Dourado

    2013-01-01

    Summary Introduction: Bullous systemic lupus erythematosus (BSLE) is an autoantibody-mediated disease with subepidermal blisters. It is a rare form of presentation of SLE that occurs in less than 5% of cases of lupus. Case Report: A 27-year-old, female, FRS patient reported the appearance of painful bullous lesions in the left nasal wing and left buccal mucosa that displayed sudden and rapid growth. She sought advice from emergency dermatology staff 15 days after onset and was hospitalized wi...

  4. Treatment of Subepidermal Autoimmune Bullous Diseases

    Directory of Open Access Journals (Sweden)

    Ekin Şavk

    2011-06-01

    Full Text Available The common therapeutic goal for all subepidermal bullous diseases is to prevent inflammation and production of pathogenic autoantibodies and/or to facilitate the elimination of these antibodies. Diseases included in this group are the pemphigoids, linear IgA dermatosis, epidermolysis bullosa acquisita, dermatitis herpetiformis and bullous systemic erythematosus. Corticosteroids are the most commonly used systemic medication. Other alternatives include dapsone, various antibiotics and nicotinamide, azathioprine, cyclophosphamide, methotrexate, cycloporine, mycophenolate mofetil, IV immunoglobulins and plasmapheresis. Rapid reepithelialization and prevention of secondary infections are provided by local hygenic measures including antiseptic baths and wound care.

  5. Reversible keratopathy due to hypertyrosinaemia following intermittent low-dose nitisinone in alkaptonuria: a case report.

    Science.gov (United States)

    Stewart, R M K; Briggs, M C; Jarvis, J C; Gallagher, J A; Ranganath, L

    2014-01-01

    We describe a patient with ultra-rare disease, alkaptonuria, who developed tyrosine keratopathy following nitisinone therapy of 2 mg on alternate days. His vision became impaired approximately 7 weeks following the commencement of nitisinone and ophthalmological examination at week nine showed characteristic dendritic keratopathy associated with tyrosinaemia. The corneal lesion as well as his visual symptoms normalized completely following discontinuation of nitisinone. This is the first documented report of keratopathy due to acquired tyrosinaemia due to very low-dose nitisinone. PMID:24997710

  6. In vivo confocal microscopy in chloroquine-induced keratopathy

    Directory of Open Access Journals (Sweden)

    Iacopo Paladini

    2013-01-01

    Full Text Available In vivo confocal microscopy is becoming a mandatory examination to study corneal abnormalities such as drug deposits in systemic disease. A female diagnosed with fibromyalgia on systemic chloroquine for 9 months presented for an ophthalmic examination. Confocal microscopy was performed using the Confoscan 4 (Nidek Co. Ltd., Gamagori, Japan and multiple highly reflective deposits in the epithelial basal cells were found, that were consistent with choloquine. Deposits were also present in the wing cell layer. In the anterior stroma these deposits were rare. Atypically shaped and branched nerves were also present in the anterior stroma. Corneal deposits of chloroquine can be evaluated by confocal microscopy. Confocal microscopy provides information on corneal metabolism and physiology. Chloroquine keratopathy can affect the anterior stroma in addition to the epithelium.

  7. Reversible Keratopathy Due to Hypertyrosinaemia Following Intermittent Low-Dose Nitisinone in Alkaptonuria: A Case Report

    OpenAIRE

    Stewart, R M K; Briggs, M C; Jarvis, J.C.; Gallagher, J.A.; Ranganath, L

    2014-01-01

    We describe a patient with ultra-rare disease, alkaptonuria, who developed tyrosine keratopathy following nitisinone therapy of 2 mg on alternate days. His vision became impaired approximately 7 weeks following the commencement of nitisinone and ophthalmological examination at week nine showed characteristic dendritic keratopathy associated with tyrosinaemia. The corneal lesion as well as his visual symptoms normalized completely following discontinuation of nitisinone. This is the first docu...

  8. Bullous disorders in Konya: A study of 93 cases

    OpenAIRE

    Munise Daye; İnci Mevlitoğlu; Sarenur Esener

    2013-01-01

    Background and Design: Bullous disorders are a group of disorders caused by specific antibodies against tissue adhesion molecules in skin and mucosa or genetic inheritance. There are limited detailed epidemiological studies about bullous disorders in Turkey. In this study we evaluated the demographic, clinical, immunopathologic, course and follow-up properties, cases in Konya. Most of cases were in pemphigus and bullous pemphigoid group, so we gave more information about them. Materials a...

  9. Regenerative approaches for the cornea.

    Science.gov (United States)

    Griffith, M; Alarcon, E I; Brunette, I

    2016-09-01

    The cornea is the transparent front part of the eye that transmits light to the back of the eye to generate vision. Loss of corneal transparency, if irreversible, leads to severe vision loss or blindness. For decades, corneal transplantation using human donor corneas has been the only option for treating corneal blindness. Despite recent improvement in surgical techniques, donor cornea transplantation remains plagued by risks of suboptimal optical results and visual acuity, immune rejection and eventually graft failure. Furthermore, the demand for suitable donor corneas is increasing faster than the number of donors, leaving thousands of curable patients untreated worldwide. Here, we critically review the state of the art of biomaterials for corneal regeneration. However, the lessons learned from the use of the cornea as a disease model will allow for extension of the biomaterials and techniques for regeneration of more complex organs such as the heart. PMID:27098482

  10. A Case With Bullous Sweet's Syndrome

    OpenAIRE

    ÇİÇEK, Demet; KANDİ, Başak; ÇOBANOĞLU, Bengü; DİLEK, Nursel

    2008-01-01

    A 46-year-old male patient who had a high number of erythematous and edematous papules, plaques and bullae on the hairy skin, neck and bilateral dorsal parts of hands, in the accompaniment of systemic conjunctival hyperemia, fever and leukocytosis was observed in the histopathological examination of skin lesions to have intensive neutrophil infiltration and bullae formation with neutrophil content in the superficial dermis, and was diagnosed as bullous Sweet's syndrome. We present th...

  11. Bullous Mastocytosis Mimicking Congenital Epidermolysis Bullosa

    OpenAIRE

    Salas-Alanis, Julio Cesar; Rosales-Mendoza, Cesar Eduardo; Ocampo-Candiani, Jorge

    2014-01-01

    A 2-month-old female infant was referred to DebRA Mexico from the Regional Children's Hospital because of a generalized dermatosis from birth characterized by multiple blisters and erosions on the trunk, face and limbs, associated with minor trauma. A skin biopsy showing subepidermal blisters associated with a dermal infiltrate of Giemsa-positive cells and CD117-positive antibody was consistent with the diagnosis of bullous mastocytosis. Treatment with oral antihistamines, topical steroids, a...

  12. ATYPICAL BULLOUS PYODERMA GANGRENOSUM WITH EARLY LESIONS MIMICKING CHICKEN POX

    OpenAIRE

    Ramesh; Kavya Raju; Gopal; Sharath Kumar; Nandini

    2013-01-01

    ABSTRACT : Pyoderma Gangrenosum (PG) rare neutrophilic dermatoses (1/100,00 0), of which Bullous Pyoderma gangrenosum is an atypical form, which is very rare. Bullous PG is usually associated with haematological disorders like myeloproliferative disorders, haematological malignancies specially AML and several other haematological disorders. It presents as a superficial haemorrhagic bulla which ulcerates, ulcers increase in size and heal with scarring. Treatmen...

  13. Bullous impetigo caused by Streptococcus salivarius: a case report.

    OpenAIRE

    Brook, I

    1980-01-01

    A 19-month-old child presented with bullous impetigo around the perineal region, penis, and left foot. Streptococcus salivarius was the only isolate recovered from the lesions. The child was treated with parenteral penicillin, debridement of the bulli, and local application of silver sulphadiazine cream. This case of bullous impetigo illustrates another aspect of the pathogenicity of Strep. salivarius.

  14. Different Nursing Care Methods for Prevention of Keratopathy Among Intensive Care Unit Patients

    Science.gov (United States)

    Kalhori, Reza Pourmirza; Ehsani, Sohrab; Daneshgar, Farid; Ashtarian, Hossein; Rezaei, Mansour

    2016-01-01

    Background: Patients with reduced consciousness level suffer from eye protection disorder and Keratopathy. This study was conducted to compare effect of three eye care techniques in prevention of keratopathy in the patients hospitalized in intensive care unit of Kermanshah. Methods: This clinical trial was conducted in 2013 with sample size of 96 persons in three random groups. Routine care included washing of eyes with normal saline and three eye care methods were conducted with poly ethylene cover, liposic ointment, and artificial tear drop randomly on one eye of each sample and a comparison was made with the opposite eye as the control. Eyes were controlled for 5 days in terms of keratopathy. Data collection instrument was keratopathy severity index. Data statistical analysis was performed with SPSS-16 software and chi-squared test, Fisher’s exact test, ANOVA and Kruskal–Wallis one-way analysis of variance. Findings: The use of poly ethylene cover (0.59±0.665) was significantly more effective in prevention of keratopathy than other methods (P=0.001). There was no statistically significant difference between two care interventions of liposic ointment and artificial tear drop (P=0.844) but the results indicated the more effective liposic ointment (1.13±0.751) than the artificial tear drop (1.59±0.875) in prevention of corneal abrasion (Phospitalized in intensive care unit.

  15. 10-Year Observation of Bullous Pemphigoid Patients

    Directory of Open Access Journals (Sweden)

    Bengu Nisa Akay

    2010-06-01

    Full Text Available Background and Design: Bullous pemphigoid (BP is an autoimmune skin disorder characterized by large, tense blisters of the skin. It occurs mainly in the elderly. The one year mortality associated with bullous pemphigoid ranges from 19% to 41%. We aim to analyse the etiologic factors, demographic features, clinical course and response to treatment in patients with BP. Material and Method: Between January 1999 and January 2008, 31 patients with BP were evaluated retrospectively. We assessed duration of the disease, presence of associated symptoms, clinical findings, drug intake, response to treatment, recurrence rates and also extensive laboratory testing and radiologic evaluations on each patient to clarify a possible underlying malignancy. Results: In a 10-year period, 31 patients with BP, female male ratio was 19/12, ranging in age from 59 to 96 (mean 78.8±8.67 years were evaluated. Duration of the disease was 16.58±18.48 months. Direct immunoflourescence of the perilesional skin showed linear deposition of IgG and C3 along the dermoepidermal junction in 24 patients (77.4%. There was no significant relationship between drug usage and bullous pemphigoid. No malignancy was detected. Medium dosage of prednisolone (50-60 mg/day was the first choice of treatment in 8 patients with a complete response rate in all but two patients died during therapy. Four patients died during hospitalization with unknown reasons before any treatment was started. In 16 cases the initial treatment was prednisolone and azathioprine. The mean follow-up period was 12 months and 11 (35% cases had relapsed. The longest remission was achieved with prednisolone and azathioprine combination. Conclusion: In conclusion, no association was found between BP and drug usage or malignancy. All patients responded well to treatment. Therapy with prednisone maintained with azathiopyrine significantly reduced the recurrences. However, disease-specific mortality is found to be high and

  16. Bullous Mastocytosis Mimicking Congenital Epidermolysis Bullosa

    Directory of Open Access Journals (Sweden)

    Julio Cesar Salas-Alanis

    2014-05-01

    Full Text Available A 2-month-old female infant was referred to DebRA Mexico from the Regional Children's Hospital because of a generalized dermatosis from birth characterized by multiple blisters and erosions on the trunk, face and limbs, associated with minor trauma. A skin biopsy showing subepidermal blisters associated with a dermal infiltrate of Giemsa-positive cells and CD117-positive antibody was consistent with the diagnosis of bullous mastocytosis. Treatment with oral antihistamines, topical steroids, and antibiotics was initiated, leading to a remission of the lesions.

  17. Erlotinib-related keratopathy in a patient underwent laser in situ keratomileusis.

    Science.gov (United States)

    Kau, Hui-Chuan; Tsai, Chieh-Chih

    2016-09-01

    Erlotinib is a tyrosine kinase inhibitor of the epidermal growth factor receptor. Since there is a wide expression of the epidermal growth factor receptors in the epithelial tissues of ocular surface and adnexa, ocular adverse reactions may happen during systemic administration of erlotinib. Previously reported ocular adverse reactions of erlotinib include trichomegaly, periorbital rash, ectropion, blepharitis, persistent corneal epithelial defect, corneal ulcer and perforation. We report the first case of erlotinib-related keratopathy in a patient who had received laser in situ keratomileusis. The patient presented a special picture of flap striae related to erlotinib. Improvement of keratopathy after cessation of erlotinib was demonstrated. PMID:26340340

  18. Bullous pemphigoid induced by vildagliptin: a report of three cases.

    Science.gov (United States)

    Béné, Johana; Jacobsoone, Aurélie; Coupe, Patrick; Auffret, Marine; Babai, Samy; Hillaire-Buys, Dominique; Jean-Pastor, Marie-Josèphe; Vonarx, Marlène; Vermersch, Annie; Tronquoy, Anne-Fleur; Gautier, Sophie

    2015-02-01

    To report three cases of bullous pemphigoid in patients treated with vildagliptin. Case 1: An 86-year-old woman presented with bullous pemphigoid after 1 month of treatment with vildagliptin and metformin. After introduction of clobetasol, the symptoms resolved although vildagliptin was continued. However, the skin lesions reappeared 3 months later. Sustained remission was achieved only after definitive withdrawal of vildagliptin. Case 2: A 79-year-old man presented with bullous pemphigoid after 37-month treatment with gliclazide, vildagliptin and metformin. The disease at first responded to clobetasol but 3 months later the lesions reappeared. They finally regressed when the gliptin was discontinued. Case 3: A 77-year-old woman, treated with gliclazide and vildagliptin for 26 months, presented with bullous pemphigoid, which responded well to discontinuation of the gliptin and topical clobetasol. Gliptins are new molecules for treatment of type 2 diabetes mellitus, which have been suspected of implication in bullous pemphigoid. Such cases have been described in the literature (seven with vildagliptin and three with sitagliptin). In nine of these cases, the gliptin was associated with metformin, but the latter had never been considered responsible. The mechanism implicated in the development of bullous pemphigoid has not yet been clearly identified, but may involve a modified immune response or alteration of the antigenic properties of the epidermal basement membrane. These reports support the risk of bullous pemphigoid in patients exposed to gliptins. PMID:24861252

  19. Bullous pemphigoid antigen localization suggests an intracellular association with hemidesmosomes

    DEFF Research Database (Denmark)

    Westgate, G E; Weaver, A C; Couchman, J R

    1985-01-01

    Autoantibodies to a normal component of stratified squamous epithelia, the bullous pemphigoid antigen (BPA), are synthesized in patients with the disease bullous pemphigoid. We have used these sera to study the distribution of BPA in vivo and in vitro. At low magnification, indirect immunofluores......Autoantibodies to a normal component of stratified squamous epithelia, the bullous pemphigoid antigen (BPA), are synthesized in patients with the disease bullous pemphigoid. We have used these sera to study the distribution of BPA in vivo and in vitro. At low magnification, indirect...... immunofluorescent staining for BPA is linear at the basement membrane zone (BMZ) of skin and many other epithelial tissues. At higher magnification however, we observed a punctate staining pattern for BPA which was regular in appearance and suggested localization of BPA to discrete structures at the BMZ. Subsequent...

  20. Bullous oral lichen planus: report of two cases

    Institute of Scientific and Technical Information of China (English)

    Berrin (U)NSAL; S. Elif G(U)LTEKIN; Erol BAL; Benay TOKMAN

    2003-01-01

    @@ Lichen planus is a common chronic mucocutaneous disease of unknown cause. Several types of lichen planus (reticular, atrophic, erosive and bullous) within the oral cavity have been described, among which the reticular and erosive types are the most common types,1,2 while the bullous type is rarely observed.1,3 Bullous lichen planus (BLP) lesions are commonly seen on the buccal mucosa, most frequently at the posterior areas adjacent to the second and third molar teeth. Less common localizations are gingiva and inner aspect of the lips.4 Bullae are generally short lived and leave ulcerated lesions on rupturing.1 The clinical diagnosis of bullous lichen planus is extremely difficult, and pathological examinations may be necessary to establish a definitive diagnosis.5

  1. Bullous impetigo caused by Streptococcus salivarius: a case report.

    Science.gov (United States)

    Brook, I

    1980-01-01

    A 19-month-old child presented with bullous impetigo around the perineal region, penis, and left foot. Streptococcus salivarius was the only isolate recovered from the lesions. The child was treated with parenteral penicillin, debridement of the bulli, and local application of silver sulphadiazine cream. This case of bullous impetigo illustrates another aspect of the pathogenicity of Strep. salivarius. Images Fig. 1 Fig. 2 PMID:7002959

  2. Evaluation of salt split technique of immunofluorescence in bullous pemphigoid

    OpenAIRE

    Satyapal Seema; Amladi Sangeeta; Jerajani H

    2002-01-01

    Recent studies suggest that salt split skin is a more sensitive substrate than intact skin for immunofluorescence diagnosis of bullous pemphigoid. We undertook this study to define the role of salt split technique of immunofluorescence findings in 32 clinical and histopothology confirmed cases of bullous pemphigoid. Both direct and indirect immunofluorescences were performed using normal and split skin. Direct immunofluorescence positivity of 100% was noted with both routine and salt s...

  3. Bullous pyoderma gangrenosum in patients with ulcerative colitis and multiple myeloma

    OpenAIRE

    Hideo Takenoshita; Toshiyuki Yamamoto

    2014-01-01

    Bullous pyoderma gangrenosum (PG) is a rare subtype of PG, which frequently involves extremities. We herein report two cases of bullous PG in association with other systemic diseases, such as hematological malignancy and inflammatory bowel disease.

  4. Medical image of the week: bullous emphysema

    Directory of Open Access Journals (Sweden)

    Tey KR

    2016-04-01

    Full Text Available No abstract available. Article truncated at 150 words. A 63-year-old gentleman, with a history of 90-pack-years of smoking and stage IV chronic obstructive pulmonary disease was receiving home oxygen at 2 L/min at baseline. He has had multiple prior hospital admissions for respiratory failure. Over the past 2 weeks he has had increased production of sputum, associated with worsening shortness of breath. He is on fluticasone-salmeterol inhaler, albuterol inhaler, and tiotropium as an outpatient. On examination, he was hemodynamically stable, SpO2 was 92% on 4L/min of oxygen. He was in obvious respiratory distress, in a tripod position with tachypnea and using respiratory accessory muscles. Lung examination revealed diffuse expiratory wheezing. Chest radiograph shows severe emphysema (Figure 1. Chest computed tomography showed diffuse centrilobular and bullous emphysema (Figure 2. He was treated as an acute severe exacerbation of COPD and was eventually discharged to follow-up with the pulmonary clinic. Emphysema is defined as alveolar destruction and airspace enlargement distal ...

  5. Genetic Background and Climatic Droplet Keratopathy Incidence in a Mapuche Population from Argentina

    OpenAIRE

    Schurr, Theodore G.; Dulik, Matthew C; Cafaro, Thamara A.; Suarez, María F.; Urrets-Zavalia, Julio A.; Serra, Horacio M.

    2013-01-01

    Purpose To determine whether the incidence of and susceptibility to climatic droplet keratopathy (CDK), an acquired, often bilateral degenerative corneal disease, is influenced by the genetic background of the individuals who exhibit the disorder. Methods To determine whether the disease expression was influenced by the genetic ancestry of CDK cases in native Mapuche of the northwest area of Patagonia in Argentina, we examined mitochondrial DNA and Y-chromosome variation in 53 unrelated indiv...

  6. Therapeutic effects of sericin on diabetic keratopathy in Otsuka Long-Evans Tokushima Fatty rats

    OpenAIRE

    Nagai, Noriaki; Ito, Yoshimasa

    2013-01-01

    An Otsuka Long-Evans Tokushima Fatty (OLETF) rat provides a useful model for studies to develop corneal wound healing drugs for use in diabetic keratopathy resulting from type 2 diabetes mellitus. We investigated the effects of sericin on corneal wound healing in OLETF rats. Corneal wounds were prepared by removal of the corneal epithelium and documented using a TRC-50X. Sericin was instilled into the eyes of rats five times a day following corneal abrasion. The plasma levels of glucose, trig...

  7. Diagnosis and classification of pemphigus and bullous pemphigoid.

    Science.gov (United States)

    Kershenovich, Ruben; Hodak, Emmilia; Mimouni, Daniel

    2014-01-01

    Pemphigus and bullous pemphigoid represent the two major groups of autoimmune blistering diseases. Pemphigus has three major variants: pemphigus vulgaris, pemphigus foliaceus and paraneoplastic pemphigus and is characterized by autoantibodies directed against the cell surface of keratinocytes, producing acantholysis that in turn leads to intraepithelial blisters in the skin and/or mucous membranes. In bullous pemphigoid, the autoantibodies are present at the dermo-epidermal junction and attack the hemidesmosomes, causing subepidermal blister formation. The classification of the major variants of both the pemphigus group and bullous pemphigoid can be based on the combination of clinical, histopathological and immunopathological criteria. Many tools are available for the diagnosis of these entities including biopsy, direct and indirect immunofluorescence, immunoprecipitation, immunoblotting and ELISA. However, currently there are no generally accepted criteria for the diagnosis of these disorders. The present review provides a proposal for diagnostic criteria. PMID:24424192

  8. Evaluation of salt split technique of immunofluorescence in bullous pemphigoid

    Directory of Open Access Journals (Sweden)

    Satyapal Seema

    2002-11-01

    Full Text Available Recent studies suggest that salt split skin is a more sensitive substrate than intact skin for immunofluorescence diagnosis of bullous pemphigoid. We undertook this study to define the role of salt split technique of immunofluorescence findings in 32 clinical and histopothology confirmed cases of bullous pemphigoid. Both direct and indirect immunofluorescences were performed using normal and split skin. Direct immunofluorescence positivity of 100% was noted with both routine and salt split method. Additional immunoreadont deposition was noted with direct method on split skin in 5 cases. Patterns of fluorescence in the latter were roof (40.60%, floor (9.4% and combined roof and floor (50%. On indirect immunofluorescence, positivity was almost doubled with salt split technique ( 68% as compared to routine method (36%. Thus, salt split technique was equivalent to routine on direct method in positivity with additional immunoreactant deposits noted in some and had double the sensitivity of the indirect method in detecting immunofluorescence in bullous pemphigoid.

  9. A Fatal Case of "Bullous Erysipelas-like" Pseudomonas Vasculitis.

    Science.gov (United States)

    Yang, Sam Shiyao; Chandran, Nisha Suyien; Huang, Jing Xiang; Tan, Kong-Bing; Aw, Derrick Chen-Wee

    2016-01-01

    Erysipelas is a generally benign superficial bacterial skin infection, and its bullous form constitutes a rare and more severe variant. We describe the first and fatal case of "bullous erysipelas-like" septic vasculitis due to Pseudomonas bacteremi. A 69-year-old Chinese man presenting with diarrhea and septic shock initially began to rapidly develop sharply defined erythematous plaques with non-hemorrhagic bullae over his lower limbs. Culture of the aspirate from the bullae was positive for Pseudomonas aeruginosa. This was also consistent with his blood cultures showing Pseudomonas bacteremia. Histology of the skin lesion showed microthrombi and neutrophilic infiltrates in blood vessels with Gram-negative bacilli extruding from the vessel walls, characteristic of septic vasculitis. The bullous erysipelas-like lesions seen in this patient represents a rare manifestation of both septic vasculitis and Pseudomonas infection. PMID:26955132

  10. A case of multiple myeloma presenting as a bullous dermatosis

    Directory of Open Access Journals (Sweden)

    Gul Ulker

    2008-01-01

    Full Text Available Multiple myeloma is a malignant plasma cell proliferative disorder that produces a monoclonal immunoglobulin protein. The skin involvement and the development of bullous disease are rarely seen features in multiple myeloma. We present a 55-year-old man with a longstanding, large, tense bullous eruption and hypertrophic scars over his body accompanied recently with weight loss and fatique. He had no response to the previous treatments, which included oral glucocorticoids and dapsone. Histologic examination of the lesions revealed subepidermal bullae, while no immunoflourescence staining was observed. In a further detailed labarotory examination, multiple myeloma was detected. After the treatment of multiple myeloma with chemotherapy, the lesions regressed. Patients with longstanding, recurrent, unusual bullous eruption should be investigated for the development of multiple myeloma.

  11. Bullous disorders in Konya: A study of 93 cases

    Directory of Open Access Journals (Sweden)

    Munise Daye

    2013-12-01

    Full Text Available Background and Design: Bullous disorders are a group of disorders caused by specific antibodies against tissue adhesion molecules in skin and mucosa or genetic inheritance. There are limited detailed epidemiological studies about bullous disorders in Turkey. In this study we evaluated the demographic, clinical, immunopathologic, course and follow-up properties, cases in Konya. Most of cases were in pemphigus and bullous pemphigoid group, so we gave more information about them. Materials and Methods: Ninty three cases that are followed up during the period of 2006-2012 were included in our study. Results: Female cases were %53,8, male cases were %46,2. The mean age was 52,9. In all of cases; %37,6 cases were pemhigus vulgaris, % 34,4 bullous pemphigoid, %15 herediter epidermolysis bullosa, %4,3 were dermatitis herpetiformis, %4,3 were pemphigus foliaceus, %2,2 pemphigus vegetans, %1,1 Hailey- Hailey disease, %1,1 cicatricial pemphigoid. Mean age of Pemhigus vulgaris onset was 45,9 year and mostly it was observed in females. Pemhigus vulgaris has been started at oral mucosae and the time for diagnosis of pemhigus vulgaris, which began at oral mucosae, was longer than other geogrophic regions. Mean age bullous pemphigoid onset was 70 year and mostly it was observed in females. In % 12,5 of cases disease started at oral mucosae. None of the cases had additional autoimmune disorder. We have treated 25 cases with systemic corticosteriods, 41 cases with systemic corticosteriods and adjuvan agents, 11 cases with only one of adjuvan agents, 16 cases with only local streoids. Conclusions: We report the demographic, clinical, immunopathologic, course and follow-up properties of the bullous disorders in Konya.

  12. Bullous Fixed Drug Eruption Probably Induced by Paracetamol

    Science.gov (United States)

    Agarwala, Manoj Kumar; Mukhopadhyay, Sramana; Sekhar, M Raja; Peter, CV Dincy

    2016-01-01

    We report a case of a 42-year-old male who presented with second episode of bullous eruptions after ingestion of paracetamol. There were no systemic complaints. The temporal correlation with the drug, history of a similar episode and the quick improvement led us to a diagnosis of bullous fixed drug due to paracetamol. Applying Naranjo's algorithm, a causality score of 8 was obtained and was categorized as probable reaction to paracetamol. Clinicians should be vigilant of the possible adverse reactions to drugs with robust safety profiles. Drug alert cards could play an important role in preventing recurrences. PMID:26951737

  13. Bullous mastocytosis in a 3-month-old infant

    Directory of Open Access Journals (Sweden)

    Dinesh Prasad Asati

    2014-01-01

    Full Text Available Mastocytosis is a rare myeloid neoplasm characterized by abnormal proliferation and accumulation of mast cells in one or more organ systems including the skin, bone marrow, liver, spleen, lymph nodes and gastrointestinal tract. An infant presenting with bullous lesions is an even rarer clinical presentation of cutaneous mastocytosis. The symptoms and complications are mostly in proportion to the mast cell degranulation in tissues. Management is focused on preventing and treating this event. We report a three-month-old infant with bullous mastocytosis to enhance awareness about this rare diagnosis.

  14. Synthetic cornea: biocompatibility and optics

    Science.gov (United States)

    Parel, Jean-Marie A.; Kaminski, Stefan; Fernandez, Viviana; Alfonso, E.; Lamar, Peggy; Lacombe, Emmanuel; Duchesne, Bernard; Dubovy, Sander; Manns, Fabrice; Rol, Pascal O.

    2002-06-01

    Purpose. Experimentally find a method to provide a safe surgical technique and an inexpensive and long lasting mesoplant for the restoration of vision in patients with bilateral corneal blindness due to ocular surface and stromal diseases. Methods. Identify the least invasive and the safest surgical technique for synthetic cornea implantation. Identify the most compatible biomaterials and the optimal shape a synthetic cornea must have to last a long time when implanted in vivo. Results. Penetrating procedures were deemed too invasive, time consuming, difficult and prone to long term complications. Therefore a non-penetrating delamination technique with central trephination was developed to preserve the integrity of Descemet's membrane and the anterior segment. Even though this approach limits the number of indications, it is acceptable since the majority of patients only have opacities in the stroma. The prosthesis was designed to fit in the removed tissue plane with its skirt fitted under the delaminated stroma. To improve retention, the trephination wall was made conical with the smallest opening on the anterior surface and a hat-shaped mesoplant was made to fit. The skirt was perforated in its perimeter to allow passage of nutrients and tissues ingrowths. To simplify the fabrication procedure, the haptic and optic were made of the same polymer. The intrastromal biocompatibility of several hydrogels was found superior to current clinically used PMMA and PTFE materials. Monobloc mesoplants made of 4 different materials were implanted in rabbits and followed weekly until extrusion occurred. Some remained optically clear allowing for fundus photography. Conclusions. Hydrogel synthetic corneas can be made to survive for periods longer than 1 year. ArF excimer laser photoablation studies are needed to determine the refractive correction potential of these mesoplants. A pilot FDA clinical trial is needed to assess the mesoplant efficacy and very long-term stability.

  15. The Clinical Diagnosis of Autoimmune Bullous Diseases Sürekli Eğitim

    Directory of Open Access Journals (Sweden)

    Rıfkiye Küçükoğlu

    2011-06-01

    Full Text Available The autoimmune bullous diseases were diagnosed on the clinical and histopathological basis, before the introduction of the immunological assays. However, not the clinical features, but the classification of the diseases has recently changed during the immunological development. According to this new classification, pemphigus group diseases include, pemphigus vulgaris, pemphigus vegetans, pemphigus foliaceus, pemphigus erythematosus, endemic pemphigus, IgA pemphigus, pemphigus herpetiformis, paraneoplastic pemphigus, and drug-induced pemphigus. The subepidermal bullous diseases are classified as pemphigoid group diseases, epidermolysis bullosa acquisita, dermatitis herpetiformis, and linear IgA bullous dermatosis. The pemphigoid-group diseases consist of bullous pemphigoid, childhood bullous pemphigoid, localised bullous pemphigoid, drug-induced pemphigoid, anti p200 pemphigoid, pemphigoid gestationes, pemphigoid nodularis, and cicatricial pemphigoid. In this review, the clinical features of the autoimmune bullous diseases are discussed according to the above mentioned classification. (Turkderm 2010; 45 Suppl 1: 16-25

  16. BULLOUS PEMPHIGOID SUCCESSFULLY CONTROLLED BY TETRACYCLINE AND NICOTINAMIDE

    NARCIS (Netherlands)

    KOLBACH, DN; REMME, JJ; BOS, WH; JONKMAN, MF; DEJONG, MCJM; PAS, HH; VANDERMEER, JB

    1995-01-01

    In 1986, Berk and Lorincz reported the efficacy of tetracycline and nicotinamide in the treatment of bullous pemphigoid (BP). In the present study of seven patients with BP, we found that a regimen of 2 g tetracycline combined with 2 g nicotinamide daily was effective in clearing the skin lesions. T

  17. Cornea procurement, preservation and transplantation

    International Nuclear Information System (INIS)

    Our experience on this subject is 40 years and we have seen the developments everywhere during this total period. Cornea procurement has been an unsurmountable problem in a large number of countries, mostly due to social and cultural reasons. The requirements for a simple eye bank to manage the supply for a local hospital are minimal. What is the most important is the donor base. In Sri Lanka nurtured in the traditions of Theravada Buddhism the donor base was easy to prepare. We had no difficulties with Christians and Hindus. For Muslims there are Fatwas in favour of donating eyes. But we always found them to be a reluctant group. But in predominantly Muslim countries, it is imperative that social workers should take up this issue, so those thousands may be helped. In all areas where attempts are made to procure comeas, it is important to have a day-and-night service available at short notice to collect any eyes offered. A trained technician with sterilised instruments to obtain eyes and blood sample must be available at a convenient location preferably in a large hospital. Even in places where the supply is infrequent, it is essential to have the readily-sterilised instruments to proceed to a donor site at short notice. To get eyes, general publicity in mass media alone is not sufficient. Such publicity makes people aware of the need for donor eyes and where a technician/doctor is available. What is more important is to canvass personally after a person has died For this purpose trained social workers may be employed, or the technician can do the motivation, as we do in Sri Lanka. Once eyes are removed, it is essential to pay careful attention to the appearance of the cadaver. It should not (and need not) cause any deformity as such event has a profound negative effect on the campaign. Many different preservation methods have been used over the years. Mc Carey-Kaufmann (MK) medium has been the standard for many years, and gives a life span of 4-5 days. The formula

  18. Coexisting Morphea and Bullous Lichen Sclerosus Et Atrophicus: A Case Report

    OpenAIRE

    Nurcan Metin; Mahizer Yaldız; Teoman Erdem; Şahin Erdem

    2015-01-01

    Morphea and lichen skleroatrofikans (LSA)are localized fibrosing diseases characterized by well-defined fibrotic plaques. Blisters rarely occur on morphea plaque, and these blisters are mostly considered as bullous morphea which is a rare type of morphea. The cases of bullous LSA growing on plaque morphea are rarer. In this case report, we presented a patient has bullous lesions compatible with of lichen skleroatrofikans clinically and histopathologically on morphea lesions, to draw attention...

  19. Coexisting Morphea and Bullous Lichen Sclerosus Et Atrophicus: A Case Report

    Directory of Open Access Journals (Sweden)

    Nurcan Metin

    2015-12-01

    Full Text Available Morphea and lichen skleroatrofikans (LSAare localized fibrosing diseases characterized by well-defined fibrotic plaques. Blisters rarely occur on morphea plaque, and these blisters are mostly considered as bullous morphea which is a rare type of morphea. The cases of bullous LSA growing on plaque morphea are rarer. In this case report, we presented a patient has bullous lesions compatible with of lichen skleroatrofikans clinically and histopathologically on morphea lesions, to draw attention to rare association of bullous morphea and LSA.

  20. Construction of Tissue Engineering Artificial Cornea with Skin Stem Cells

    Institute of Scientific and Technical Information of China (English)

    Yuan LIU; Yan JIN

    2005-01-01

    @@ 1 Introduction The clinical need for an alternative to donor corneal tissue has encouraged much interests in recent years. An artificial cornea must fulfill the functions of the cornea it replaces. More recently, the idea of a bio-engineered cornea has risen. Corneal equivalents have been reconstructed by tissue engineering method. Aim of this study is to construct an artificial rabbit cornea by employing tissue engineering method and to determine if skin stem cells have a role in tissue engineered cornea construction.

  1. Diagnosis and clinical severity markers of bullous pemphigoid

    OpenAIRE

    Schmidt, Enno; Zillikens, Detlef

    2009-01-01

    The use of a broad spectrum of novel detection systems for autoantibodies to the basement membrane proteins BP180 and BP230 has greatly facilitated the diagnosis of bullous pemphigoid, which most likely explains its increasing incidence in central Europe. Because the pathogenic relevance of antibodies to human BP180 has been convincingly shown both in vitro and in vivo, repeated testing for these antibodies appears to be helpful in guiding treatment decisions during the course of the disease.

  2. Drug-Induced Bullous Sweet Syndrome with Multiple Autoimmune Features

    OpenAIRE

    2010-01-01

    Sweet syndrome (SS) (Acute Febrile Neutrophilic Dermatosis) has been reported in association with autoimmune phenomena including relapsing polychondritis, drug-induced lupus, and the development of antineutrophil cytoplasmic antibodies (ANCAs). However, a combination of these autoimmune features has not been reported. Herein, we report a case of drug-induced bullous SS with ocular and mucosal involvement, glomerulonephritis, and multiple autoimmune features including clinical polychondritis w...

  3. A Rare Case of Pilomatricoma with Bullous Appearance

    OpenAIRE

    Karadağ, Ayşe Serap; EKŞİOĞLU, Meral; ÖZLÜK, Ekin; AKBAY, Gülfer

    2009-01-01

    Background: Pilomatricoma is a rare, benign adnexal tumor differentiating toward elements of the hair matrix and shaft. It typically presents as a solitary, deep, dermal nodule. In addition, bullous changes can be rarely seen. Methods: A 16-year-old girl with firm, mobile, multilobule and non-tender mass that has a soft, hemorrhagic blister-like formation on the left infraclavicular region is described. Results: The histopathologic findings were consistent with pilomatricoma. In the dermis...

  4. Bullous mastocytosis in a 3-month-old infant

    OpenAIRE

    Dinesh Prasad Asati; Anurag Tiwari

    2014-01-01

    Mastocytosis is a rare myeloid neoplasm characterized by abnormal proliferation and accumulation of mast cells in one or more organ systems including the skin, bone marrow, liver, spleen, lymph nodes and gastrointestinal tract. An infant presenting with bullous lesions is an even rarer clinical presentation of cutaneous mastocytosis. The symptoms and complications are mostly in proportion to the mast cell degranulation in tissues. Management is focused on preventing and treating this event. W...

  5. Bullous Darier’s disease mimicking Hailey-Hailey disease

    OpenAIRE

    Khaled, Aida; Ben Hamida, Myriam; Goucha, Samia; Zermani, Rachida; Rameh, Soumaya; Ben Taazayet, Sabra; Kamoun, Mohamed Ridha; Fazaa, Bécima

    2011-01-01

    Background The bullous variant of Darier’s disease (DD) is a rare type characterized by histological and clinical overlap with Hailey-Hailey disease (HDD). Case reports The following case report describes two cases of familial DD; a 51-year-old woman who presented with erythematous plaques, covered by small blisters in axillary and inguinal areas, and the first patient’s daughter, who presented with keratotic papules localized on the axillary and inframammary folds. Conclusion These two cases...

  6. ATYPICAL BULLOUS PYODERMA GANGRENOSUM WITH EARLY LESIONS MIMICKING CHICKEN POX

    Directory of Open Access Journals (Sweden)

    Ramesh

    2013-10-01

    Full Text Available ABSTRACT : Pyoderma Gangrenosum (PG rare neutrophilic dermatoses (1/100,00 0, of which Bullous Pyoderma gangrenosum is an atypical form, which is very rare. Bullous PG is usually associated with haematological disorders like myeloproliferative disorders, haematological malignancies specially AML and several other haematological disorders. It presents as a superficial haemorrhagic bulla which ulcerates, ulcers increase in size and heal with scarring. Treatment is mainly to identify and treat the cause. Pyoderma Gangrenosum shows rapid response to oral corticosteroid therapy. 1 , 3 . Clinical presentation: A 32yr old female presented with fever, multiple vesicles on face, upper limb, lower limb and trunk, and these early lesions looked like chicken pox lesions. Lesions increased in size to form haemorrhagic bullas which eroded to form ulcers, ulcers rapidly increased in size with necrotic base and erythematous to violaceous border. Investigation: Haemoglobin: 5.7gm%, Peripheral smear: normocytic and normochromic anaemia. Skin Biopsy: Sub corneal blisters with dermal and perifollicular n eutrophilic infiltrate. A diagnosis of Bullous Pyoderma gangrenosum was made. Patient’s anaemia was treated; oral prednisolone and topical steroids were started. Patient showed marked improvement to treatment.

  7. Nummular keratopathy in a patient with Hyper-IgD Syndrome

    Directory of Open Access Journals (Sweden)

    Culican Susan M

    2009-08-01

    Full Text Available Abstract Purpose To report a case of recurrent nummular keratitis in a pediatric patient with Hyperimmunoglobulinemia D syndrome. Methods A retrospective chart review. Results A 14-year-old boy with Hyperimmunoglobulinemia D syndrome (HIDS presented with photophobia and ocular irritation concomitant with disease exacerbation. He was found on exam to have significant nummular keratitis, which responded to a short course of topical steroids. Despite acute response to local immunosuppression, the patient had several recurrent attacks and eventually developed a large corneal scar and decreased vision. After initiation of infliximab therapy his ocular sequelae improved dramatically and his vision returned to 20/20. Conclusion One possible form of end-organ damage associated with HIDS is vision threatening nummular keratopathy.

  8. In vivo ultrathin Descemet stripping automated endothelial keratoplasty with a low-energy and high-frequency femtosecond laser

    Directory of Open Access Journals (Sweden)

    Gustavo Victor

    2014-04-01

    Full Text Available This case report describes the production of an ultrathin endothelial donor corneal lamella using a femtosecond laser with low energy and a high frequency. In addition, we report its use in vivo in an eye with pseudophakic bullous keratopathy. The outcomes were observed 3 months after surgery in terms of the change in endothelial donor lamella and full cornea thickness (including pachymetric mapping, visual acuity, and endothelial cell count.

  9. The Effect and Experience of Animal Cornea Application in Ophthalmology Trainee%眼角膜疾病见习中的教学效果和体会

    Institute of Scientific and Technical Information of China (English)

    杨硕; 李学东; 华文; 卢弘

    2016-01-01

    在眼科教学中,传统的角膜病见习内容往往不能达到良好的教学效果。在近年的教学中,进行了见习内容改革,将动物眼球作为教具引入眼科见习中,增加了实际解剖操作、基本显微操作等内容,并通过具体病例分析角膜移植的临床意义、介绍国内外角膜器官捐献现状并结合医学伦理学进行讨论。从中,提高了医学生的学习兴趣,培养了医学生的观察力和动手能力,同时还培养了学员的医学伦理思维,这种见习模式获得了理想的教学效果。%In the past, courses of cornea mainly focused on the theoretical knowledge and cognition of keratopathy. Students always feel so tasteless about the course. Therefore, traditional courses can’ t achieve good teaching effects. In the recent years, we try to change this teaching mode and adjust course contents: including application of animal cornea to replace the traditional teaching aids, the actual dissection, microscopic operation, analysis on significance of cornea transplantation, introduction of domestic and foreign cornea organ donation status and discussion of medical ethics issues. The new teaching method has obtained ideal effects.

  10. Case Report of Bullous Pemphigoid following Fundus Fluorescein Angiography

    Directory of Open Access Journals (Sweden)

    Goktug Demirci

    2010-05-01

    Full Text Available Purpose: To report a first case of bullous pemphigoid (BP following intravenous fluorescein for fundus angiography. Clinical Features: A 70-year-old male patient was admitted to the intensive care unit with BP and sepsis. He reported a history of fundus fluorescein angiography with a pre-diagnosis of senile macular degeneration 2 months prior to presentation. At that time, fluorescein extravasated at the antecubital region. Following the procedure, pruritus and erythema began at the wrists bilaterally, and quickly spread to the entire body. The patient also reported a history of allergy to human albumin solution (Plamasteril®; Abbott 15 years before, during bypass surgery. On dermatologic examination, erythematous patches were present on the scalp, chest and anogenital region. Vesicles and bullous lesions were present on upper and lower extremities. On day 2 of hospitalization, tense bullae appeared on the upper and lower extremities. The patient was treated with oral methylprednisolone 48 mg (Prednol®; Mustafa Nevzat, topical clobetasol dipropionate 0.05% cream (Dermovate®; Glaxo SmithKline, and topical 4% urea lotion (Excipial Lipo®; Orva for presumptive bullous pemphigoid. Skin punch biopsy provided tissue for histopathology, direct immunofluorescence examination, and salt extraction, which were all consistent with BP. After 1 month, the patient was transferred to the intensive care unit with sepsis secondary to urinary tract infection; he died 2 weeks later from sepsis and cardiac failure. Conclusions: To our knowledge, this is the first reported case of BP following fundus fluorescein angiography in a patient with known human albumin solution allergy. Consideration should be made to avoid fluorescein angiography, change administration route, or premedicate with antihistamines in patients with known human albumin solution allergy. The association between fundus fluorescein angiography and BP should be further investigated.

  11. Bullous lesions, sweat gland necrosis and rhabdomyolysis in alcoholic coma

    Directory of Open Access Journals (Sweden)

    Neelakandhan Asokan

    2014-01-01

    Full Text Available A 42-year-old male developed hemorrhagic bullae and erosions while in alcohol induced coma. The lesions were limited to areas of the body in prolonged contact with the ground in the comatose state. He developed rhabdomyolysis, progressing to acute renal failure (ARF. Histopathological examination of the skin showed spongiosis, intraepidermal vesicles, and necrosis of eccrine sweat glands with denudation of secretory epithelial lining cells. With supportive treatment and hemodialysis, the patient recovered in 3 weeks time. This is the first reported case of bullous lesions and sweat gland necrosis occurring in alcohol-induced coma complicated by rhabdomyolysis and ARF.

  12. Drug-Induced Bullous Sweet Syndrome with Multiple Autoimmune Features

    Directory of Open Access Journals (Sweden)

    Jared J. Lund

    2010-01-01

    Full Text Available Sweet syndrome (SS (Acute Febrile Neutrophilic Dermatosis has been reported in association with autoimmune phenomena including relapsing polychondritis, drug-induced lupus, and the development of antineutrophil cytoplasmic antibodies (ANCAs. However, a combination of these autoimmune features has not been reported. Herein, we report a case of drug-induced bullous SS with ocular and mucosal involvement, glomerulonephritis, and multiple autoimmune features including clinical polychondritis with antitype II collagen antibodies, ANCAs, antinuclear (HEp-2, and antihistone antibodies in a patient on hydralazine and carbamazepine.

  13. Airway management in a patient with bullous pemphigoid

    International Nuclear Information System (INIS)

    Airway management in patients with pemphigoid lesions has anaesthetic implications. We report a case of a 23 years old female with bullous pemphigoid who presented with laryngeal stenosis and critical airway narrowing. The airway was initially managed with jet ventilation. Anaesthesia was maintained with propofol infusion and ventilation was performed by introducing a size 10 French gauge suction catheter through the stenotic laryngeal orifice. Thirty minutes into anaesthesia, she developed subcutaneous emphysema and decreased air entry on right side of the chest but remained hemodynamically stable. The airway was further managed by tracheostomy. This case report highlights complications that can occur during the anaesthetic management of such cases. (author)

  14. Two percent ethylenediaminetetraacetic acid chelation treatment for band-shaped keratopathy, without blunt scratching after removal of the corneal epithelium

    OpenAIRE

    Kobayashi W; Yokokura S; Hariya T; Nakazawa T

    2015-01-01

    Wataru Kobayashi,1 Shunji Yokokura,1 Takehiro Hariya,1 Toru Nakazawa1–3 1Department of Ophthalmology, 2Department of Retinal Disease Control, Ophthalmology, 3Department of Advanced Ophthalmic Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan Background: The purpose of this study was to assess the effectiveness of 2% ethylenediaminetetraacetic acid (EDTA) for the treatment of band-shaped keratopathy.Methods: We studied 24 eyes of 16 patients with band-shaped kerato...

  15. PSEUDOSINDACTILIA EN EPIDERMÓLISIS BULLOSA Pseudosyndactylia in bullous epidermolysis

    Directory of Open Access Journals (Sweden)

    Enríque Vergara Amador

    2009-01-01

    Full Text Available La epidermólisis bullosa comprende un grupo de patologías que se caracterizan por la fragilidad de la piel, formación de ampollas, y en las formas distróficas, la formación de pseudosindactilias. Debido a lo exótico de la entidad y la dificultad para el enfoque de tratamiento, se presenta un caso clínico con desarrollo de pseudosindactilias, característico usualmente de las formas distróficas. Se describe en aspectos clínicos de la entidad, el tratamiento quirúrgico de las pseudosindactilias y de las contracturas en las manos, y se discuten aspectos de la enfermedad y de los tipos de tratamientoBullous epidermolysis comprises a group of pathologies characterized by skin fragility, formation of bullae and, in its dystrophic forms, by the formation of pseudosyndactylias. Due to the exotic nature of the disease and the difficulty in focusing its treatment, a case is presented in which pseudosyndactylias developed, a usual characteristic of the dystrophic forms. This article addresses clinical aspects of bullous epidermolysis, surgical treatment of pseudosyndactilia and hand contractures and other aspects of the disease and its treatment

  16. Bullous Pemphigoid: A Rare Childhood Disease with Eleveted Immunoglobulin E Levels

    Directory of Open Access Journals (Sweden)

    Hayrullah Alp

    2010-03-01

    Full Text Available Bullous pemphigoid is a rare pemphigoid disease characterized by separation at the dermal-epidermal junction and common blisters clinically. We have presented a 4 month-old case who developed tense bullae during her first month of life and diagnosed as bullous pemphigoid with elevated serum IgE levels. In spite of the elevated serum IgE levels, the patient received benefit from the topical steroid treatment. Also, this is the youngest case of bullous pemphigoid that is not associated with the maternal pemphigoid gestationis.

  17. Recent advancement on autoantigens, autoantibodies and inflammatory cells in subepidermal autoimmune bullous diseases

    Institute of Scientific and Technical Information of China (English)

    Jianguo Huang; Qiying Gong; Guiming Li

    2007-01-01

    Subepidermal autoimmune bullous diseases (SABD) are some autoimmune skin diseases that can present in a variety of forms and can be a challenging disease to treat. An overview of the different forms of SABD are discussed including bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA), cicatricial pemphigoid (CP), bullous systemic lupus erythematosus (BSLE), and Anti-p200 pemphigoid. Emphasis on recent advancement is presented. In recent years, improved Knowledge of the mechanisms of intercellular and cell-matrix adhesion has led to better understanding of the blistering process in some SABD. Defects of such structures cause the subepidermal bullous diseases and have also led to the discovery of new diseases (e.g. anti-p200-pemphigoid). Recent studies have outlined the important role of autoantibodies, mast cell lymphocytes and their cytokines in pathogenesis of SABD.

  18. Alternative approach to fit irregular corneas

    OpenAIRE

    Espinosa Tomás, Julián; Pérez Rodríguez, Jorge; Mas Candela, David; Vázquez Ferri, Carmen; Illueca Contri, Carlos

    2010-01-01

    Comunicación presentada en 5th European Meeting on Visual and Physiological Optics (EMVPO), Stockholm, 22-24 August 2010. We propose a zonal Zernike fitting (combination of zonal and modal approaches) of corneal height data. It permits accurate analysis of the surface, diminishing the influence of smooth areas over irregular zones and vice versa. This fact will be of special interest in irregular corneas wavefront evaluation. This work has been supported by the Generalitat Valenciana pr...

  19. Bullous mastocytosis in an infant associated with the use of a nonprescription cough suppressant.

    Science.gov (United States)

    Cook, J; Stith, M; Sahn, E E

    1996-01-01

    Bullous mastocytosis is an unusual expression of mastocytosis typically seen in young children, and many causes of the acute mast cell degranulation with bulla formation have been identified. We report a 6-month-old boy with urticaria pigmentosa and an extensive bullous eruption associated with the ingestion of a nonprescription cough suppressant containing dextromethorphan. The pathogenesis of mastocytosis and the care of patients with this disease are discussed. PMID:8893244

  20. Recommendations for the use of immunoapheresis in the treatment of autoimmune bullous diseases.

    Science.gov (United States)

    Zillikens, Detlef; Derfler, Kurt; Eming, Rüdiger; Fierlbeck, Gerhard; Goebeler, Matthias; Hertl, Michael; Hofmann, Silke C; Karlhofer, Franz; Kautz, Ocko; Nitschke, Martin; Opitz, Andreas; Quist, Sven; Rose, Christian; Schanz, Stefan; Schmidt, Enno; Shimanovich, Iakov; Michael, Michael; Ziller, Fabian

    2007-10-01

    Despite the use of high-dose systemic corticosteroids in combination with other immunosuppressants, in some patients with autoimmune bullous diseases only insufficient improvement is achieved. In these cases and in acute severe disease, adjuvant immunoapheresis has been increasingly used. A consensus meeting was held in mid-2005 in Hamburg, aiming at developing guidelines for the use of immunoapheresis in the treatment of autoimmune bullous diseases. This paper summarizes the experts' recommendations. PMID:17910670

  1. Treatment of Subepidermal Autoimmune Bullous Diseases Sürekli Eğitim

    OpenAIRE

    Ekin Şavk

    2011-01-01

    The common therapeutic goal for all subepidermal bullous diseases is to prevent inflammation and production of pathogenic autoantibodies and/or to facilitate the elimination of these antibodies. Diseases included in this group are the pemphigoids, linear IgA dermatosis, epidermolysis bullosa acquisita, dermatitis herpetiformis and bullous systemic erythematosus. Corticosteroids are the most commonly used systemic medication. Other alternatives include dapsone, various antibiotics and nicotina...

  2. Dental Treatment of a Child Suffering from Non-bullous Congenital Ichthyosiform Erythroderma under General Anesthesia

    OpenAIRE

    Choudhary, Rahul; Satish, V.

    2015-01-01

    ABSTRACT Non-bullous congenital ichthyosiform erythroderma (NBCIE) is an autosomal recessive form of inherited icthyosis appears as fine white scales that gradually replace collodion membrane. This case report describes management of 5 years and 11-month-old child with NBCIE suffering from early childhood caries (ECC) under general anesthesia. How to cite this article: Choudhary R, Satish V. Dental Treatment of a Child Suffering from Non-bullous Congenital Ichthyosiform Erythroderma under Gen...

  3. Bullous emphysema as first presentation of Ehlers-Danlos syndrome in monozygotic twins

    OpenAIRE

    Paolo Ruggeri; Salvatore Calcaterra; Giuseppe Girbino

    2014-01-01

    Ehlers-Danlos syndrome, characterized by hyperextensible skin, hypermobile joints, and fragile vessels, is the most common heritable disorder of connective tissue and has an estimated prevalence of 1 in 5000. Pulmonary involvement with signs of lung destruction (bullous emphysema) as first presentation is unusual. We report a case of monozygotic twins 37 years old men with occasional evidence of bullous emphysema with previously undiagnosed Ehlers-Danlos syndrome type IV. We emphasize the imp...

  4. Bullous pemphigoid after allogeneic hematopoietic stem cell transplantation.

    Science.gov (United States)

    Kato, Keisuke; Koike, Kazutoshi; Kobayashi, Chie; Iijima, Shigeruko; Hashimoto, Takashi; Tsuchida, Masahiro

    2015-06-01

    Bullous pemphigoid (BP) is an autoimmune skin disorder characterized by subepidermal blisters due to deposit of autoantibody against dermal basement membrane protein. It has been reported that BP can occur after allogeneic hematopoietic stem cell transplantation (HSCT). We describe a patient with BP having autoantibody against BP180 after unrelated-donor HSCT against T lymphoblastic leukemia. The patient was treated with steroid leading to complete resolution of BP, but T lymphoblastic leukemia progressed rapidly after steroid hormone treatment. Given that immunosuppressant may reduce graft-versus-tumor effect, immunomodulatory agents such as nicotinamide and tetracycline, erythromycin, and immunoglobulin may be appropriate as soon as typical blister lesions are seen after HSCT. PMID:26113316

  5. VANCOMYCIN-INDUCED LINEAR IGA BULLOUS DERMATOSIS WITH ISOMORPHIC RESPONSE

    Directory of Open Access Journals (Sweden)

    Tatsuhiko Mori

    2013-11-01

    Full Text Available Linear IgA bullous dermatosis (LABD is occasionally induced by certain drugs, of which vancomycin is the most common. We herein describe a case of vancomycin-induced LABD on the trunk and extremities. Our case was unique in which tense bulla was induced on the old operation scars. A 92-year-old man developed diffuse erythema and bullas on his trunk and extremities. Also, blister formation was observed on the operation scar on the abdomen. A biopsy specimen showed subepidermal split with neutrophilic and lymphocytic infiltration in the upper dermis. Direct immunofluorescence showed a linear IgA deposition at the basement membrane zone. His skin conditions were improved by stoppage of vancomycin and topical corticosteroids. We should know about the occurrence of LABD in patients under vancomycin treatment.

  6. Bleb Point: Mimicker of Pneumothorax in Bullous Lung Disease

    Directory of Open Access Journals (Sweden)

    Gelabert, Christopher

    2015-05-01

    Full Text Available In patients presenting with severe dyspnea, several diagnostic challenges arise in distinguishing the diagnosis of pneumothorax versus several other pulmonary etiologies like bullous lung disease, pneumonia, interstitial lung disease, and acute respiratory distress syndrome. Distinguishing between large pulmonary bullae and pneumothorax is of the utmost importance, as the acute management is very different. While multiple imaging modalities are available, plain radiographs may be inadequate to make the diagnosis and other advanced imaging may be difficult to obtain. Ultrasound has a very high specificity for pneumothorax. We present a case where a large pulmonary bleb mimics the lung point and therefore inaccurately suggests pneumothorax. [West J Emerg Med. 2015;16(3:447–449.

  7. Dermatoses bolhosas auto-imunes Autoimmune bullous dermatoses

    Directory of Open Access Journals (Sweden)

    Paulo R. Cunha

    2009-04-01

    Full Text Available Dermatoses bolhosas autoimunes são doenças cuja manifestação cutânea primária e fundamental consiste em vesículas e bolhas. Classificam-se conforme a localização da bolha, em intraepidérmica e subepidérmica. Os pacientes produzem autoanticorpos contra estruturas específicas da pele detectáveis por técnicas de imunofluorescência, immunobloting e Elisa. Os recentes avanços da biologia molecular e celular têm permitido conhecer esses autoantígenos, contra os quais os pacientes se sensibilizam e que estão localizados na epiderme ou na junção dermoepidérmica. São doenças de baixa incidência, porém de elevada morbidade e por vezes letais. O objetivo deste trabalho é revisar e descrever os progressos nos conhecimentos de quatro doenças vésico-bolhosas autoimunes: pênfigo foliáceo endêmico (fogo selvagem, pênfigo vulgar, penfigóide bolhoso e dermatite herpetiforme.Autoimmune bullous dermatoses are diseases in which blisters and vesicles are the primary and fundamental types of skin lesion. Their classification is based on the location of the blister: intraepidermal and subepidermal. Patients produce autoantibodies against self-specific structures of the skin detectable by immunofluorescence techniques, immunoblotting and ELISA. Recent advances in molecular and cellular biology have brought to knowledge these self-antigens, against which patients are sensitized, and which are found in epidermis or in the dermo-epidermal junction. These are low incidence, but high morbidity diseases that may be fatal. The aim of this article is to review and describe the progress of four autoimmune vesiculobullous disorders: endemic pemphigus foliaceous (wild fire, pemphigus vulgaris, bullous pemphigoid and dermatitis herpetiformis.

  8. Evidence for herpes simplex viral latency in the human cornea.

    OpenAIRE

    Kaye, S. B.; Lynas, C.; Patterson, A.; Risk, J. M.; McCarthy, K.; Hart, C. A.

    1991-01-01

    Patients undergoing penetrating keratoplasty for prior herpes simplex keratitis (group A) and corneal disease unrelated to herpes simplex (group B) were investigated to assess whether the cornea is a site for herpes simplex viral latency. All patients were seropositive for herpes simplex viral antibody. Virus was isolated from the tear film postoperatively in one patient and on cocultivation from the cornea of another patient. Herpes simplex viral DNA, however, was detected in the corneas of ...

  9. Early cystic bleb needling revision after glaucoma filtering surgery with toxic keratopathy

    Directory of Open Access Journals (Sweden)

    S. Yu. Petrov

    2014-01-01

    Full Text Available Clinical case of high thin-wall cystic limited filtering bleb needling revision in the early post-op period after trabeculectomy with sinusotomy accompanied by toxic keratopathy is presented. Optical coherence tomography (OCT demonstrated that filtering bleb height was 2700 μm and bleb wall thickness was 70 μm. Bleb needling revision with its lateralwall dissection and subconjunctival injection of dexamethasone, fluorouracil, and ranibizumab near to the bleb site was performed. In 1.5 hours after the procedure, bleb height decreased to 550 μm (by 5 times while bleb wall thickness increased up to 100 μm. Topical antibacterial, steroid, and non-steroid anti-inflammatory therapy was recommended. The next day IOP level reduced from 11 mm Hg to 4.5 mm Hg. It was accompanied by choroidal effusion that was managed conservatively with cycloplegic agents (drops and injections for 3 days. On day 6, central corneal edema affecting all layers, Descemet’s membrane folds, and ocular hypertension were revealed. Metabolic therapy resolved corneal edema within 3 days. Re-needling bleb revision decreased IOP level to 6.2 mm Hg. This resulted in transient Descemet’s membrane folds. This paper describes filtering bleb needling revision with its lateral wall dissection and anti-inflammatory, cytostatic, and anti-VEGF agents use to prolong glaucoma filtering surgery effect in excessive scarring. The procedure was accompanied by toxic corneal endothelium decompensation with corneal edema and Descemet’s membrane folds treated with active metabolic therapy.

  10. Early cystic bleb needling revision after glaucoma filtering surgery with toxic keratopathy

    Directory of Open Access Journals (Sweden)

    S. Yu. Petrov

    2015-01-01

    Full Text Available Clinical case of high thin-wall cystic limited filtering bleb needling revision in the early post-op period after trabeculectomy with sinusotomy accompanied by toxic keratopathy is presented. Optical coherence tomography (OCT demonstrated that filtering bleb height was 2700 μm and bleb wall thickness was 70 μm. Bleb needling revision with its lateralwall dissection and subconjunctival injection of dexamethasone, fluorouracil, and ranibizumab near to the bleb site was performed. In 1.5 hours after the procedure, bleb height decreased to 550 μm (by 5 times while bleb wall thickness increased up to 100 μm. Topical antibacterial, steroid, and non-steroid anti-inflammatory therapy was recommended. The next day IOP level reduced from 11 mm Hg to 4.5 mm Hg. It was accompanied by choroidal effusion that was managed conservatively with cycloplegic agents (drops and injections for 3 days. On day 6, central corneal edema affecting all layers, Descemet’s membrane folds, and ocular hypertension were revealed. Metabolic therapy resolved corneal edema within 3 days. Re-needling bleb revision decreased IOP level to 6.2 mm Hg. This resulted in transient Descemet’s membrane folds. This paper describes filtering bleb needling revision with its lateral wall dissection and anti-inflammatory, cytostatic, and anti-VEGF agents use to prolong glaucoma filtering surgery effect in excessive scarring. The procedure was accompanied by toxic corneal endothelium decompensation with corneal edema and Descemet’s membrane folds treated with active metabolic therapy.

  11. Dendritic cell dysfunction and diabetic sensory neuropathy in the cornea.

    Science.gov (United States)

    Gao, Nan; Yan, Chenxi; Lee, Patrick; Sun, Haijing; Yu, Fu-Shin

    2016-05-01

    Diabetic peripheral neuropathy (DPN) often leads to neurotrophic ulcerations in the cornea and skin; however, the underlying cellular mechanisms of this complication are poorly understood. Here, we used post-wound corneal sensory degeneration and regeneration as a model and tested the hypothesis that diabetes adversely affects DC populations and infiltration, resulting in disrupted DC-nerve communication and DPN. In streptozotocin-induced type 1 diabetic mice, there was a substantial reduction in sensory nerve density and the number of intraepithelial DCs in unwounded (UW) corneas. In wounded corneas, diabetes markedly delayed sensory nerve regeneration and reduced the number of infiltrating DCs, which were a major source of ciliary neurotrophic factor (CNTF) in the cornea. While CNTF neutralization retarded reinnervation in normal corneas, exogenous CNTF accelerated nerve regeneration in the wounded corneas of diabetic mice and healthy animals, in which DCs had been locally depleted. Moreover, blockade of the CNTF-specific receptor CNTFRα induced sensory nerve degeneration and retarded regeneration in normal corneas. Soluble CNTFRα also partially restored the branching of diabetes-suppressed sensory nerve endings and regeneration in the diabetic corneas. Collectively, our data show that DCs mediate sensory nerve innervation and regeneration through CNTF and that diabetes reduces DC populations in UW and wounded corneas, resulting in decreased CNTF and impaired sensory nerve innervation and regeneration. PMID:27064280

  12. Awareness of Cornea Donation of Registered Tissue Donors in Nanjing

    Institute of Scientific and Technical Information of China (English)

    Ting Chu; Lin-nong Wang; Hao Yu; Ru-yang Zhang

    2013-01-01

    Objective To evaluate the current cornea donation awareness of tissue donors in the city of Nanjing,China. Methods Altogether 2000 registered tissue donors in the Red Cross Eye Bank of Nanjing by the end of 2010 and 2000 control residents of Nanjing in February to June 2011 were randomly selected to par-ticipate in our field questionnaire survey. The questionnaire consisted of questions regarding the understand-ing of cornea donation,the attitude toward cornea donation,and attitude toward legislation and free dona-tion. The awareness of cornea donation between the registered tissue donors and residents was compared. Related factors of the willingness to donate corneas and to become a tissue donor were evaluated with uni-variate and multiple logistic regression analysis. Results A total of 1867 (response rate: 93.4%) tissue donors and 1796 (response rate: 89.8%; ef-fective questionnaires: 1697) residents participated in this survey. For the questions about the knowledge of cornea donation,90.3% tissue donors (residents: 78.9%) knew that donated corneas could be used for transplantations; 71.2% tissue donors (residents: 47.6%) knew that the appearance would not be destroyed after cornea donation; 70.7% tissue donors (residents: 20.0%) knew the formalities to become a cornea do-nor. For attitude toward cornea donation,82.2% tissue donors (residents: 45.1%) were willing to donate corneas or eyeballs after death; 84.0% tissue donors (residents: 30.2%) had discussed with their families about donation; 85.1% tissue donors (residents: 24.8%) supported their families' or friends' cornea donation. For attitude toward legislation and free donation,88.3% tissue donors (residents: 61.3%) approved of legis-lation to regular cornea donation; 72.2% tissue donors (residents: 38.8%) thought that cornea or organ do-nation should be gratis. The difference between two groups was significant (P<0.001). However,some tissue donors did not know cornea donation well,some even opposed the

  13. Investigation of Friction-induced Damage to the Pig Cornea

    NARCIS (Netherlands)

    da Cruz Barros, Raquel; Van Kooten, Theo G.; Veeregowda, Deepak Halenahally

    2015-01-01

    Mechanical friction causes damage to the cornea. A friction measurement device with minimal intervention with the pig cornea tear film revealed a low friction coefficient of 0.011 in glycerine solution. Glycerine molecules presumably bind to water, mucins, and epithelial cells and therewith improve

  14. Refractive analysis of the human cornea through propagated fields

    OpenAIRE

    Illueca Contri, Carlos; Mas Candela, David; Pérez Rodríguez, Jorge; Pons Moreno, Álvaro Máximo; Artigas Verde, José María

    2000-01-01

    A new technique for analysing the optical quality of the human cornea is presented. Corneal maps are obtained through keratographies and then converted into phase maps. The propagated fields generated from this surface are plotted and studied. It is shown that any irregularity in the cornea affects the propagated field and the energy distribution at the focal plane. Simple applications are also indicated.

  15. Azathioprine-induced bullous Sweet's syndrome: a rare association.

    Science.gov (United States)

    Biswas, Sugata Narayan; Chakraborty, Partha Pratim; Gantait, Kripasindhu; Bar, Chittaranjan

    2016-01-01

    A 52-year-old man presented with high-grade fever, headache and painful vesicular skin rash involving the upper trunk and upper extremities, 8 days after initiation of chemotherapy with azathioprine (50 mg/day), which had been prescribed for acral vitiligo. There was neither any history of preceding respiratory or gastrointestinal tract infection, nor was the patient known to have malignancy, drug hypersensitivity, inflammatory bowel disease, vasculitis or other autoimmune disease. Laboratory results revealed leucocytosis with neutrophilia and markedly elevated acute phase reactants. Antinuclear antibody, perinuclear and cytoplasmic antineutrophil cytoplasmic antibody were found negative. Punch biopsy from skin of the upper trunk revealed dense neutrophilic infiltration of dermis without signs of vasculitis, suggestive of Sweet's syndrome. In view of the temporal association with azathioprine and absence of an obvious alternative aetiology, provisional diagnosis of drug-induced bullous Sweet's syndrome was made. Azathioprine was discontinued and high-dose oral prednisolone initiated. The response was dramatic with resolution of skin lesions within 72 h without further recurrence at fourth week of follow-up. PMID:27090551

  16. Bullous variant of familial biphasic lichen amyloidosis: A unique combination of three rare presentations

    Directory of Open Access Journals (Sweden)

    Vijayalaxmi Veerabasappa Suranagi

    2015-01-01

    Full Text Available A 55-year-old man presented with multiple, itchy papules and macules on the trunk and extremities. Histopathologic examination of biopsy specimens taken from three different lesions showed a subepidermal blister with amyloid deposits in the dermal papillae. No systemic disease or involvement of other organs was detected. The clinical and histological findings were compatible with a bullous variant of lichen amyloidosis (LA. Primary cutaneous localized amyloidosis usually presents with papular, macular or nodular lesions. Bullous lesions associated with LA are very rare. Furthermore, patient had seven other members in the family with similar lesions, which is also a rare occurrence. We report a case with a rare combination of biphasic, bullous variant of familial LA.

  17. Bullous emphysema as first presentation of Ehlers-Danlos syndrome in monozygotic twins

    Directory of Open Access Journals (Sweden)

    Paolo Ruggeri

    2015-01-01

    Full Text Available Ehlers-Danlos syndrome, characterized by hyperextensible skin, hypermobile joints, and fragile vessels, is the most common heritable disorder of connective tissue and has an estimated prevalence of 1 in 5000. Pulmonary involvement with signs of lung destruction (bullous emphysema as first presentation is unusual. We report a case of monozygotic twins 37 years old men with occasional evidence of bullous emphysema with previously undiagnosed Ehlers-Danlos syndrome type IV. We emphasize the importance of considering uncommon genetic causes of emphysema in young adults, discuss underlining pathophysiological mechanisms and propose a conservative management and follow-up.

  18. Treatment of Subepidermal Autoimmune Bullous Diseases Sürekli Eğitim

    Directory of Open Access Journals (Sweden)

    Ekin Şavk

    2011-06-01

    Full Text Available The common therapeutic goal for all subepidermal bullous diseases is to prevent inflammation and production of pathogenic autoantibodies and/or to facilitate the elimination of these antibodies. Diseases included in this group are the pemphigoids, linear IgA dermatosis, epidermolysis bullosa acquisita, dermatitis herpetiformis and bullous systemic erythematosus. Corticosteroids are the most commonly used systemic medication. Other alternatives include dapsone, various antibiotics and nicotinamide, azathioprine, cyclophosphamide, methotrexate, cycloporine, mycophenolate mofetil, IV immunoglobulins and plasmapheresis. Rapid reepithelialization and prevention of secondary infections are provided by local hygenic measures including antiseptic baths and wound care. (Turkderm 2011; 45 Suppl 1: 54-8

  19. Customized Finite Element Modelling of the Human Cornea.

    Directory of Open Access Journals (Sweden)

    Irene Simonini

    Full Text Available To construct patient-specific solid models of human cornea from ocular topographer data, to increase the accuracy of the biomechanical and optical estimate of the changes in refractive power and stress caused by photorefractive keratectomy (PRK.Corneal elevation maps of five human eyes were taken with a rotating Scheimpflug camera combined with a Placido disk before and after refractive surgery. Patient-specific solid models were created and discretized in finite elements to estimate the corneal strain and stress fields in preoperative and postoperative configurations and derive the refractive parameters of the cornea.Patient-specific geometrical models of the cornea allow for the creation of personalized refractive maps at different levels of IOP. Thinned postoperative corneas show a higher stress gradient across the thickness and higher sensitivity of all geometrical and refractive parameters to the fluctuation of the IOP.Patient-specific numerical models of the cornea can provide accurate quantitative information on the refractive properties of the cornea under different levels of IOP and describe the change of the stress state of the cornea due to refractive surgery (PRK. Patient-specific models can be used as indicators of feasibility before performing the surgery.

  20. The anisotropic material constitutive models for the human cornea.

    Science.gov (United States)

    Li, Long-yuan; Tighe, Brian

    2006-03-01

    This paper presents an anisotropic analysis model for the human cornea. The model is based on the assumption that the fibrils in the cornea are organised into lamellae, which may have preferential orientation along the superior-inferior and nasal-temporal directions, while the alignment of lamellae with different orientations is assumed to be random. Hence, the cornea can be regarded as a laminated composite shell. The constitutive equation describing the relationships between membrane forces, bending moments, and membrane strains, bending curvatures are derived. The influences of lamella orientations and the random alignment of lamellae on the stiffness coefficients of the constitutive equation are discussed. PMID:16426861

  1. Crystalline-Like Keratopathy after Intravenous Immunoglobulin Therapy with Incomplete Kawasaki Disease: Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Elif Erdem

    2013-01-01

    Full Text Available A 7-year-old girl had presented with high body temperature and joint pain which continued for 3 days. Because of the prolonged history of unexplained fever, rash, bilateral nonpurulent conjunctival injection, oropharyngeal erythema, strawberry tongue, and extreme of age, incomplete Kawasaki disease was considered and started on an intravenous immunoglobulin infusion. Six days after this treatment, patient was referred to eye clinic with decreased vision and photophobia. Visual acuity was reduced to 20/40 in both eyes. Slit-lamp examination revealed bilateral diffuse corneal punctate epitheliopathy and anterior stromal haze. Corneal epitheliopathy seemed like crystal deposits. One day after presentation, mild anterior uveitis was added to clinical picture. All ocular findings disappeared in one week with topical steroid and unpreserved artificial tear drops. We present a case who was diagnosed as incomplete Kawasaki disease along with bilateral diffuse crystalline-like keratopathy. We supposed that unusual ocular presentation may be associated with intravenous immunoglobulin treatment.

  2. The effectiveness of thin-section computed tomography in diagnosing bullous lesions in patients with spontaneous pneumothorax

    International Nuclear Information System (INIS)

    We investigated the effectiveness of this-section computed tomography (CT) for the diagnosis of bullous lesions in patients with spontaneous pneumothorax. The study group consisted of 74 patients. Apical regions of the lung were scanned for lesions by thin-section CT prior to operation. The presence, number, and locations of bullous lesions were assessed. Bullous lesions were also classified into 2 groups according to their shape as demonstrated by CT findings. Operative findings confirmed that 73 of the 74 patients had bullous lesions. Of these, 33 had a single bulla and 40 had multiple bulla. Thin-section CT accurately detected the presence and location of the bullous lesions, accurately identified the number in all but 6 patients with multiple bulla (accuracy: 91.8%), and also classified them accurately by type. Precise evaluations of bullous lesions are crucial to the treatment of patients with spontaneous pneumothorax. The presence of bullous lesions, as well as their location and shape, are important factors in determining whether to operate or not. Such information also allows for a better understanding of the surgical options available. Our study demonstrated that thin-section CT of apical regions of the lung is an effective diagnostic procedure for patients with spontaneous pneumothorax. ((author)

  3. The effectiveness of thin-section computed tomography in diagnosing bullous lesions in patients with spontaneous pneumothorax

    Energy Technology Data Exchange (ETDEWEB)

    Yasufuku, Kazuhiro; Oiwa, Takashi [Higashi-Matsudo Municipal Hospital, Chiba (Japan); Fujisawa, Takehiko

    1999-12-01

    We investigated the effectiveness of this-section computed tomography (CT) for the diagnosis of bullous lesions in patients with spontaneous pneumothorax. The study group consisted of 74 patients. Apical regions of the lung were scanned for lesions by thin-section CT prior to operation. The presence, number, and locations of bullous lesions were assessed. Bullous lesions were also classified into 2 groups according to their shape as demonstrated by CT findings. Operative findings confirmed that 73 of the 74 patients had bullous lesions. Of these, 33 had a single bulla and 40 had multiple bulla. Thin-section CT accurately detected the presence and location of the bullous lesions, accurately identified the number in all but 6 patients with multiple bulla (accuracy: 91.8%), and also classified them accurately by type. Precise evaluations of bullous lesions are crucial to the treatment of patients with spontaneous pneumothorax. The presence of bullous lesions, as well as their location and shape, are important factors in determining whether to operate or not. Such information also allows for a better understanding of the surgical options available. Our study demonstrated that thin-section CT of apical regions of the lung is an effective diagnostic procedure for patients with spontaneous pneumothorax. ((author)

  4. Bullous Pemphigoid in Iranian Patients: A Descriptive Study on 122 Cases

    Directory of Open Access Journals (Sweden)

    Cheyda Chams Davatchi

    2012-05-01

    Full Text Available Bullous pemphigoid is an immunobullous disease with high mortality and morbidity. Different aspects and characteristics in the patients vary in different areas in the world. Our objective was to study clinical and demographic characteristics of bullous pemphigoid in Iranian patients. In a retrospective descriptive study, we reviewed 122 patients with bullous pemphigoid within 1987-2007. Demographic characteristics, clinical manifestations, treatment, relapses and outcome were evaluated. The mean age of 122 patients was 65 ± 18.11 years including 35.2% male and 64.8% female. The most common manifestations were cutaneous bullae (97.5%. 27% had oral lesions. 30.3% had eosinophillia. 90 patients(73.8% received oral prednisolone, 29 patients (23.8% topical steroid, 2 patients tetracycline and 1 patient dapsone. 89 patients were followed after admission. Out of them 44 patients experienced first relapse and 22 patients second relapse. 41 cases (46% were completely controlled. 11 cases (12% were not controlled. Clinical and general characteristics of bullous pemphigoid patients differ in various regions in the world.

  5. Evidence-based treatments for pemphigus vulgaris, pemphigus foliaceus, and bullous pemphigoid: A systematic review

    Directory of Open Access Journals (Sweden)

    Sanjay Singh

    2011-01-01

    Full Text Available Background: Pemphigus, bullous pemphigoid, and epidermolysis bullosa acquisita are autoimmune diseases of skin associated with considerable morbidity and sometimes mortality. There is no cure for these diseases. Aims: To summarize evidence-based treatments for these diseases by performing a systematic review. Methods: The research protocol included the following steps: identification of databases to be searched, defining search strategy, searching the databases for references, first-stage screening of the abstracts, second-stage screening of full texts of articles identified after the first-stage screening, data extraction from the identified articles after second-stage screening, quality appraisal of the studies using the Delphi list, and summarizing the findings. Results: No randomized controlled trials of interventions in pemphigus vegetans, pemphigus erythematosus, and epidermolysis bullosa acquisita could be found. After the second-stage screening, 12 randomized controlled trials were analyzed, which included patients with pemphigus vulgaris or pemphigus vulgaris and pemphigus foliaceus, and 7 which included patients with bullous pemphigoid. Conclusions: Number of high-quality randomized controlled trials conducted on pemphigus and bullous pemphigoid is small. Oral corticosteroid along with a steroid-sparing agent appears to be the most effective treatment for pemphigus. Azathioprine may be most effective as a steroid-sparing agent. Topical corticosteroid therapy (as studied is effective for bullous pemphigoid and appears to be superior to oral corticosteroid for extensive disease. Some suggestions about future research are made.

  6. Bullous reaction to a Mantoux test; a case report and review of the literature

    OpenAIRE

    Daifullah Al Aboud2

    2016-01-01

    The tuberculin skin test is widely used in the diagnosis of latent tuberculosis infection Blistering skin lesion from the test is rarely observed. In this manuscript, I report a patient who develops a bullous lesion from the test and I review the related literature.

  7. Changing pattern of utilization of human donor cornea in India

    Directory of Open Access Journals (Sweden)

    Varun Gogia

    2015-01-01

    Full Text Available Purpose: To review the changing pattern of donor, corneal utilization in an eye bank at a Tertiary Care Center in Northern India by analyzing the trend in the years 2003, 2008, and 2011. Methods: A retrospective review of eye bank records for 3 years (2003, 2008, and 2011 was performed at the National Eye Bank. Details including a clinical grade of donor cornea, indication of corneal transplantation (therapeutic or optical, type of procedure (penetrating or lamellar keratoplasty [LK], and clinical diagnosis of the graft recipients were recorded. Primary outcome measure was to observe any preference toward LK, judicious usage of donor corneal tissue, and impact of lamellar corneal transplant in the usage of donor corneas. Secondary outcomes included overall utilization rate and change in trend of indication for keratoplasty. Results: A total of 673, 745, and 864 corneas were retrieved in the years 2003, 2008, and 2011, respectively. The percentage of donor corneal utilization increased significantly over time with the rate being 65.08%, 70.06%, and 68.29%, respectively, in the years 2003, 2008, and 2011 (P = 0.014; however, this change was reflected only in the usage of nonoptical grade corneas and not for the optical grade corneas. There was an overall increase in lamellar corneal procedures for any clinical grade of cornea (P = 0.0019; number of Descemet's stripping automated endothelial keratoplasty (DSAEK procedures increased significantly (P < 0.001, particularly for pseudophakic corneal edema (PCE (P = 0.0085 and failed graft (P = 0.002. Significant increase in the utilization of nonoptical grade corneas was observed over the years (P = 0.005, though the utilization did not increase significantly for optical purposes viz., LK (P = 0.08. Conclusions: Utilization rate of donor corneas increased over the years, primarily due to increase in usage of nonoptical grade corneas for therapeutic purposes. There was a procedural shift toward DSAEK for

  8. Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea

    OpenAIRE

    Boote, Craig; Hayes, Sally; Robert D. Young; Kamma-Lorger, Christina S.; Hocking, Paul M.; Elsheikh, Ahmed; Inglehearn, Chris F.; Ali, Manir; Meek, Keith M.

    2009-01-01

    In the cornea, the precise organisation of fibrillar collagen and associated proteoglycans comprising the stromal extracellular matrix plays a major role in governing tissue form and function. Recently, abnormal collagen alignment was noted in the misshapen corneas of mature chickens affected by the retinopathy, globe enlarged (rge) mutation. Here we further characterize corneal ultrastructural changes as the rge eye develops post-hatch. Wide-angle X-ray scattering disclosed alteration to dom...

  9. CORRECTION OF MYOPIA USING CORNEA SPARING LASIK (ABLATION ON FLAP)

    OpenAIRE

    Nikhilesh; Vikas; Atul; Sudha,; Chitra

    2015-01-01

    Cornea sparing lasik is useful tool allowing surgeons to preserve the posterior stroma. The study comprised of prospective evaluation of 17 eyes treated with CSL – Cornea Sparing Lasik at Mahatme Eye Bank Eye Hospital , N agpur India. Our results show that the laser ablation on the corneal flap is safe and effective procedure. The refractive , efficacy and safety outcomes were similar to those in routine Lasik ablation on posterior stroma.

  10. CORRECTION OF MYOPIA USING CORNEA SPARING LASIK (ABLATION ON FLAP

    Directory of Open Access Journals (Sweden)

    Nikhilesh

    2015-02-01

    Full Text Available Cornea sparing lasik is useful tool allowing surgeons to preserve the posterior stroma. The study comprised of prospective evaluation of 17 eyes treated with CSL – Cornea Sparing Lasik at Mahatme Eye Bank Eye Hospital , N agpur India. Our results show that the laser ablation on the corneal flap is safe and effective procedure. The refractive , efficacy and safety outcomes were similar to those in routine Lasik ablation on posterior stroma.

  11. Collagen cross-linking in thin corneas

    Directory of Open Access Journals (Sweden)

    Prema Padmanabhan

    2013-01-01

    Full Text Available Collagen cross-linking (CXL has become the standard of care for progressive keratoconus, after numerous clinical studies have established its efficacy and safety in suitably selected eyes. The standard protocol is applicable in eyes which have a minimum corneal thickness of 400 μm after epithelial debridement. This prerequisite was stipulated to protect the corneal endothelium and intraocular tissues from the deleterious effect of ultraviolet-A (UVA radiation. However, patients with keratoconus often present with corneal thickness of less than 400 μm and could have otherwise benefited from this procedure. A few modifications of the standard procedure have been suggested to benefit these patients without a compromise in safety. Transepithelial cross-linking, pachymetry-guided epithelial debridement before cross-linking, and the use of hypoosmolar riboflavin are some of the techniques that have been attempted. Although clinical data is limited at the present time, these techniques are worth considering in patients with thin corneas. Further studies are needed to scientifically establish their efficacy and safety.

  12. Ultraviolet laser effects on the cornea

    Science.gov (United States)

    Zuclich, Joseph A.

    1990-07-01

    Ultraviolet radiation in the ambient environment or from artificial sources may pose both acute and chronic hazards to the skin and the ocular tissues. In general terrestrial conditions have evolved such that there are only narrow safety margins between ambient UV levels and exposure levels harmful to the human. Obvious examples of acute consequences ofUV overexposure are sunburn and snowblindness as well as analogous conditions induced by artificial sources such as the welder''s arc mercury vapor lamps and UV-emitting lasers. Further chronic UV exposure is strongly implicated as a causative agent in certain types of cataract and skin cancer. This presentation will summarize a number of specific cases where UV radiation affected the primate cornea. Data presented will include the action spectra for far- and near-UV induced ocular damage the pulsewidth and total energy dependencies of ocular thresholds studies of cumulative effects of repeated UV exposures and quantitative determinations of tissue repair or recovery rates. Depending on the exposure parameters utilized photochemical thermal or photoablative damage mechanisms may prevail. 1.

  13. Femtosecond lasers for microsurgery of cornea

    Energy Technology Data Exchange (ETDEWEB)

    Vartapetov, Sergei K; Khudyakov, D V; Lapshin, Konstantin E; Obidin, Aleksei Z; Shcherbakov, Ivan A

    2012-03-31

    The review of femtosecond laser installations for medical applications is given and a new femtosecond ophthalmologic system for creation of a flap of corneal tissue during the LASIK operation is described. An all-fibre femtosecond laser emitting {approx}400-fs pulses at 1067 nm is used. The pulse repetition rate can vary from 200 kHz up to 1 MHz. The output energy of the femtosecond system does not exceed 1 {mu}J. A specially developed objective with small spherical and chromatic aberrations is applied to focus laser radiation to an area of an eye cornea. The size of the focusing spot does not exceed 3 {mu}m. To process the required area, scanning by a laser beam is applied with a speed no less than 5 m s{sup -1}. At a stage of preliminary tests of the system, the {Kappa}8 glass, organic PMMA glass and specially prepared agarose gels are used as a phantom of an eye. The femtosecond system is successfully clinically tested on a plenty of eyes of a pig and on several human eyes. The duration of the procedure of creation of a corneal flap does not exceed 20 s.

  14. Glycosaminoglycans in the Human Cornea: Age-Related Changes

    Science.gov (United States)

    Pacella, Elena; Pacella, Fernanda; De Paolis, Giulio; Parisella, Francesca Romana; Turchetti, Paolo; Anello, Giulia; Cavallotti, Carlo

    2015-01-01

    AIM To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides. METHODS Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed. RESULTS The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects. DISCUSSION GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea. PMID:25674020

  15. Matrix metalloproteinase expression in excimer laser wounded rabbit corneas

    Science.gov (United States)

    Hahn, Taewon; Chamon, Wallace; Akova, Yonja; Stark, Walter J.; Stetler-Stevenson, William G.; Azar, Dimitri T.

    1994-06-01

    This study was performed to obtain information about matrix metalloproteinase (MMP) expression in excimer-wounded corneas and to determine whether MMPs expression correlates with the depth of the ablation. 6-mm excimer keratectomy (60 or 180 micrometers ) was performed using the 193-mm ArF excimer laser on 12 NZW rabbits. Corneas treated with mechanical epithelial debridement and untreated corneas served as controls. Rabbits were killed at 20 and 30 hr after laser ablation. Zymography after SDS extraction was performed on regenerated central epithelium and the central stroma to determine MMPs expression. We observed enzymatic activity of a 92 KDa band in the epithelium of excimer-ablated corneas but not in that following debridement wounds and untreated controls. The expression of the 92 KDa MMP was most pronounced with the deeper excimer ablation. A 72 KDa band of enzymatic activity present in the stroma of all treated and control eyes was also seen in the epithelium of excimer-ablated corneas. These proteolytic enzymes may play an important role in wound healing and remodelling after excimer keratectomy.

  16. Three-year observation of the patients with bullous pemphigoid: 29 cases

    OpenAIRE

    Özlem Ekiz; Bilge Bülbül Şen; Emine Nur Rifaioğlu; Tuğba Şen; Esin Atik; Asena Çiğdem Doğramacı

    2013-01-01

    Background and Design: Bullous pemphigoid (BP) is a chronic, autoimmune, subepidermal blistering disease. The aim of this study was to determine the clinico-epidemiologic data’s of patients diagnosed as BP in our clinic and compare these data with other studies in our country and in the world. Material and Method: We researched the documents of BP cases diagnosed by clinical and histopathological examinations from January 2009 to January 2013, retrospectively. We evaluated clinical and de...

  17. Aberrant Expression and Secretion of Heat Shock Protein 90 in Patients with Bullous Pemphigoid

    OpenAIRE

    Stefan Tukaj; Konrad Kleszczyński; Katerina Vafia; Stephanie Groth; Damian Meyersburg; Piotr Trzonkowski; Ludwig, Ralf J; Detlef Zillikens; Enno Schmidt; Tobias W Fischer; Michael Kasperkiewicz

    2013-01-01

    The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP), the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i) Hsp9...

  18. Definitions and outcome measures for bullous pemphigoid: Recommendations by an international panel of experts

    OpenAIRE

    Murrell, Dedee F.; Daniel, Benjamin S; Joly, Pascal; Borradori, Luca; Amagai, Masayuki; HASHIMOTO, TAKASHI; Caux, Frédéric; Marinovic, Branka; Sinha, Animesh A.; Hertl, Michael; Bernard, Philippe; Sirois, David; Cianchini, Giuseppe; Fairley, Janet A.; Jonkman, Marcel F.

    2011-01-01

    Our scientific knowledge of bullous pemphigoid (BP) has dramatically progressed in recent years. However, despite the availability of various therapeutic options for the treatment of inflammatory diseases, only a few multicenter controlled trials have helped to define effective therapies in BP. A major obstacle in sharing multicenter-based evidences for therapeutic efforts is the lack of generally accepted definitions for the clinical evaluation of patients with BP. Common terms and end point...

  19. Evidence-based treatments for pemphigus vulgaris, pemphigus foliaceus, and bullous pemphigoid: A systematic review

    OpenAIRE

    Sanjay Singh

    2011-01-01

    Background: Pemphigus, bullous pemphigoid, and epidermolysis bullosa acquisita are autoimmune diseases of skin associated with considerable morbidity and sometimes mortality. There is no cure for these diseases. Aims: To summarize evidence-based treatments for these diseases by performing a systematic review. Methods: The research protocol included the following steps: identification of databases to be searched, defining search strategy, searching the databases for references, first-stage scr...

  20. Diffuse cutaneous mastocytosis with bullous lesions and pulmonary involvement: A rare case

    Directory of Open Access Journals (Sweden)

    Sandipan Dhar

    2015-01-01

    Full Text Available Mastocytosis is defined as a heterogeneous group of disorders characterized by an accumulation of mast cells in one or more organs, particularly in the skin, bone marrow, liver, spleen and lymph nodes. However here we describe an 11-month-old girl child presented with diffuse cutaneous mastocytosis with bullous lesion, having pulmonary involvement, which is very rare and there is no available case report from India.

  1. Diffuse Cutaneous Mastocytosis with Bullous Lesions and Pulmonary Involvement: A Rare Case

    OpenAIRE

    Sandipan Dhar; Biplab Maji; Swapan Roy; Subhra Dhar

    2015-01-01

    Mastocytosis is defined as a heterogeneous group of disorders characterized by an accumulation of mast cells in one or more organs, particularly in the skin, bone marrow, liver, spleen and lymph nodes. However here we describe an 11-month-old girl child presented with diffuse cutaneous mastocytosis with bullous lesion, having pulmonary involvement, which is very rare and there is no available case report from India.

  2. Frequency of IgA antibodies in pemphigus, bullous pemphigoid and mucous membrane pemphigoid.

    Science.gov (United States)

    Cozzani, Emanuele; Drosera, Massimo; Parodi, Aurora; Carrozzo, Marco; Gandolfo, Sergio; Rebora, Alfredo

    2004-01-01

    Circulating and bound IgA antibodies can be found in the autoimmune blistering diseases, but their prevalence, clinical relevance and target antigens remain unknown. Thirty-two patients with pemphigus, 73 with bullous pemphigoid and 28 with mucous membrane pemphigoid were studied retrospectively. Direct immunofluorescence (DIF) analysis of IgG, IgA, IgM and C3 was carried out for all cases. Sera were studied by standard indirect immunofluorescence, indirect immunofluorescence on salt-split skin, immunoblotting for bullous pemphigoid and mucous membrane pemphigoid and ELISA for pemphigus. With DIF, we found IgA autoantibodies in 22 of all 133 cases. Circulating IgA antibodies to skin were detected in 2 of 3 IgA-DIF-positive patients with pemphigus, in 3 of 6 with bullous pemphigoid, and in 6 of 13 with mucous membrane pemphigoid. We confirm that the IgA reactivity is more frequently associated with mucous membrane involvement, especially in cases without critical involvement (5/8). The role of IgA and its antigenic specificity in these diseases remain unclear. PMID:15370705

  3. Expression of Interleukin 1 Receptor Antagonist in Human Cornea

    OpenAIRE

    Heur, Martin; Shyam S. Chaurasia; Wilson, Steven E.

    2008-01-01

    The purpose of this study was to confirm the expression of interleukin-1 receptor antagonist (IL-1 Ra) in the human cornea. Four samples of human ex vivo corneal epithelium were obtained from patients undergoing photorefractive keratectomy. RT-PCR was performed using mRNA isolated from the corneal epithelium and oligo-dT primers. PCR was performed on the cDNA products using primers specific for human IL-1Ra. The PCR products were subcloned and sequenced. Human cornea sections were prepared fr...

  4. 1H high resolution magic angle spinning NMR spectroscopy of rabbit cornea

    Czech Academy of Sciences Publication Activity Database

    Saether, O.; Risa, O.; Čejková, Jitka; Krane, J.; Midelfart, A.

    Prague: organizing committee, 2002. s. 38. [International Symposium on Cornea and Contact Lenses.. 07.12.2002-10.12.2002, Prague] Institutional research plan: CEZ:AV0Z5039906 Keywords : cornea Subject RIV: FF - HEENT, Dentistry

  5. The transparent lens and cornea in the mouse and zebra fish eye

    OpenAIRE

    Greiling, Teri M.S.; Clark, John I.

    2007-01-01

    The lens and cornea combine to form a single optical element in which transparency and refraction are the fundamental biophysical characteristics required for a functional visual system. Although lens and cornea have different cellular and extracellular specializations that contribute to transparency and refraction, their development is closely related. In the embryonic mouse, the developing cornea and lens separate early. In contrast, zebra fish lens and cornea remain connected during early ...

  6. Effect of Seawater Soaking on Explosive Cornea Injury

    Institute of Scientific and Technical Information of China (English)

    Suihua Chen; Zhenping Huang; Lili Wang; Yuwen Lu; Yi Wang

    2002-01-01

    Objective: To observe the changes in corneal tissue after explosive corneal injury and the effect of seawater soaking on the healing of cornea after explosive injury with the help ofoptical microscope. Methods: Make 10 similar explosive injury models of rabbit′s eyeball using 10 adult greyrabbits. For each rabbit, both eyes are artificially injured through explosion; its right eye is the comparison eye and after the injury the left eye is soaked in seawater for 30 minutes.Conduct slit lamp examinations, fluorescein dyeing, and ultrasonic cornea thickness metering on the corneas at intervals, that is, before the injury, 1 , 2, 3, 4, 5, 7, 9, 12, 15days after the injury respectively. Examine the corneas under the optical microscope andmake comparisons.Results: The corneal thickness of the experiment eye becomes apparently thicker than thatof the comparison eye after the injury. The corneal clouding of the former apparentlyaggravates compared with the latter. The healing of corneal epithelium in the injured eye isslower compared with that in the comparison eye. Comparative examination under the opticalmicroscope shows: after the injury, the corneal tissue of the experiment eye changesdistinctly, its healing is delayed and the vascularization degree becomes high in cornealstroma.Conclusion: Scars and vascularization of various degrees will appear in corneal stroma afterexplosive injuries. Seawater soaking will have bad effects on the healing of the corneainjured in explosions.

  7. Xanthine oxidoreductase and xantine oxidase in human cornea

    Czech Academy of Sciences Publication Activity Database

    Čejková, Jitka; Ardan, Taras; Filipec, M.; Midelfart, A.

    2002-01-01

    Roč. 17, - (2002), s. 755-760. ISSN 0213-3911 R&D Projects: GA ČR GA304/00/1635; GA ČR GV307/96/K226 Institutional research plan: CEZ:AV0Z5039906 Keywords : human cornea * xanthine oxidoreductase in situ Subject RIV: FF - HEENT, Dentistry Impact factor: 1.881, year: 2002

  8. Penfigoide bolhoso no adulto mais jovem: relato de três casos Bullous pemphigoid in younger adults: three case reports

    Directory of Open Access Journals (Sweden)

    Roberta Richter Zanella

    2011-04-01

    Full Text Available O penfigoide bolhoso é uma dermatose bolhosa autoimune subepidérmica, mais comumente observada na população idosa (acima dos 70 anos. Autoanticorpos são formados contra antígenos específicos da zona de membrana basal: BP180 e BP230 (proteínas do hemidesmossomo. Apresentamos três casos de penfigoide bolhoso, em adultos com menos de 50 anos de idade, destacan do as peculiaridades clínicas na faixa etária mais jovem.Bullous pemphigoid is an autoimmune subepidermal bullous dermatosis more commonly observed in the elderly (over 70 years old. Autoantibodies are produced for specific antigens of the epidermal basement membrane zone: BP 180 and BP 230 (hemidesmosome proteins. We report three cases of bullous pemphigoid in adults younger than 50 years old, discussing the clinical characteristics of the disease in younger patients.

  9. Bullous Pemphigoid With a Dual Pattern of Glomerular Immune Complex Disease.

    Science.gov (United States)

    Hoorn, Ewout J; Taams, Noor E; Hurskainen, Tiina; Salih, Mahdi; Weening, Jan J; Jonkman, Marcel F; Pas, Hendri H; Schreurs, Marco W J

    2016-02-01

    A 75-year-old man presented with a blistering skin disease and nephrotic syndrome. Bullous pemphigoid was diagnosed by linear immunoglobulin G (IgG) and C3 staining along the basement membrane zone of a skin biopsy specimen and by the presence of circulating IgG recognizing the 180-kDa bullous pemphigoid antigen (BP180; type XVII collagen). A kidney biopsy specimen showed endocapillary inflammation without crescents. Direct immunofluorescence showed strong IgG and C3 staining in a combined granular and linear pattern along the glomerular basement membrane. Electron microscopy showed subepithelial deposits. In serum, no antibodies against the Goodpasture antigen (type IV collagen) or phospholipase A2 receptor were detected. Indirect immunofluorescence studies using the patient's serum showed a strikingly linear but not granular IgG pattern along the epithelial basement membranes of monkey esophagus and kidney. Although type XVII collagen was recently identified in the glomerulus, the patient's serum did not produce a 180-kDa band on immunoblot of kidney tissue and still stained glomeruli of BP180 knockout mice by indirect immunofluorescence. The patient was treated with prednisone and azathioprine, which resulted in complete remission of skin and kidney manifestations. Although bullous pemphigoid has been reported previously in association with anti-glomerular basement membrane disease or membranous nephropathy, this case demonstrates both elements in 1 patient. This concurrence and the linear pattern on indirect immunofluorescence support the possibility of cross-reactive or parallel autoantibodies to basement membranes with a secondary membranous component. PMID:26616334

  10. The Use of Traditional Chinese Medicine in Some Dermatologic Diseases: Part II--Autoimmune Bullous Disorders and Lichen Planus.

    Science.gov (United States)

    Baroni, Adone; Ruocco, Eleonora; Russo, Teresa; Piccolo, Vincenzo; Geng, Long; Zhou, Hongbo; Chen, Hong-Duo; Gao, Xing-Hua

    2015-01-01

    Over the centuries, traditional Chinese medicine (TCM) and Western medicine have traveled along parallel lines with no opportunity for collaboration. In recent decades, while an interest in TCM has been growing among Western clinicians, progress has been made in the comprehension of pathogenic mechanisms of skin disorders, and the communication between Western and Eastern medicines has become more and more intensive. In this paper, the authors discuss TCM remedies used in the treatment of autoimmune bullous disorders (pemphigus and bullous pemphigoid) and lichen planus. Future studies on the active components of the TCM will certainly shed new light on the still obscure aspects of some dermatologic diseases. PMID:26380506

  11. Recurrent bilaterally symmetrical bullous sweet′s syndrome: A rare and confusing entity

    Directory of Open Access Journals (Sweden)

    Shyam B Verma

    2012-01-01

    Full Text Available A rare presentation of Sweet′s syndrome (SS (acute febrile neutrophilic dermatosis is being reported in a 28-year-old man from India. I report a rare presentation of SS that consisted of bilaterally symmetrical, bullous lesions on both forearms with a seasonal recurrence. A thorough work-up for the usual conditions predisposing to or leading to SS did not throw any light on the etiology. After failing treatment with prednisolone alone we added indomethacin, and he responded to the combination in a matter of days with complete clearance of lesions.

  12. Localized bullous pemphigoid on sites of radiotherapy and lymphedema in the same patient

    Directory of Open Access Journals (Sweden)

    Manikoth P Binitha

    2014-01-01

    Full Text Available Bullous pemphigoid (BP is a blistering disorder due to autoantibodies to the epidermal basement membrane zone. The triggering factor could be localized damage to the skin by physical or chemical agents. We report a case of a 68-year-old woman with a three year history of oral lesions of BP following radiotherapy for carcinoma of the hypopharynx, and a three month history of BP over lymphedematous sites on the right hand and right lower limb. Localized BP induced by radiotherapy or lymphedema is rare; both factors working simultaneously in the same patient is even rarer.

  13. Erythema nodosum as azathioprine hypersensitivity reaction in a patient with bullous pemphigoid

    Directory of Open Access Journals (Sweden)

    Jose Antonio Vargas-Hitos

    2013-01-01

    Full Text Available A 65-year-old woman with bullous pemphigoid presented with fever and several red-purple nodular subcutaneous lesions on both lower legs 1 week after starting treatment with azathioprine (AZA. Biopsy of a skin nodule was compatible with erythema nodosum (EN and hypersensitivity reaction to AZA was suspected. AZA was subsequently discontinued, observing complete remission of fever and EN within 2 weeks. This case highlights the importance of recognizing EN as a possible manifestation of hypersensitivity reaction to AZA.

  14. Live imaging of newly formed lymphatic vessels in the cornea

    Institute of Scientific and Technical Information of China (English)

    Don Yuen; Xiufeng Wu; Alex C Kwan; Jeffrey LeDue; Hui Zhang; Tatiana Ecoiffier; Bronislaw Pytowski; Lu Chen

    2011-01-01

    Dear Editor,Lymphatic research denotes a field of new discovery and has experienced exponential growth in recent years [1-3].Though lymphatic dysfunction has been found in a broad spectrum of disorders from transplant rejection to cancer metastasis,to date,there is still little effective treatment for lymphatic diseases,so it is a field with urgent demand for new experimental approaches and therapeutic protocols.The cornea provides an ideal site for lymphatic research due to its accessible location,transparent nature,and alymphatic status under normal condition [2,4].Indeed,the use of this tissue for tumor angiogenesis research dates back to 1970s [5].Most recently,we have demonstrated that the cornea possesses a full range of plasticity in lymphatic formation and regression [6].An advanced technology for live imaging of lymphatic vessels in this tissue would therefore have widespread applications in biomedical research.

  15. Analysis of Cornea Transplant Tissue Rejection Delay in Mice Subjects

    Czech Academy of Sciences Publication Activity Database

    Valenta, Zdeněk; Svozílková, P.; Filipec, M.; Zvárová, Jana; Farghali, H.

    Berlin: Springer, 2004 - (Barreiro, J.; Martin-Sanchez, F.; Maojo, V.), s. 292-298. (Lecture Notes in Computer Science. 3337). ISBN 3-540-23964-2. [ISBMDA. International Symposium /5./. Barcelona (ES), 18.11.2004-19.11.2004] R&D Projects: GA ČR GP305/03/D130; GA MZd NI7531; GA MŠk LN00B107 Keywords : cornea transplant * immunosuppressant * hazard ratio Subject RIV: BB - Applied Statistics, Operational Research

  16. Ultrastructural changes in the developing chicken cornea following caffeine administration.

    OpenAIRE

    Bartel Hieronim; Tosik Dariusz; Kujawa-Hadryś Monika

    2010-01-01

    Caffeine is one of the most frequently consumed psychoactive substances. It has been known for many years that caffeine at high concentrations exerts harmful effects on both women's and laboratory animals' fertility, moreover it may impair normal development of many organs in the prenatal period. So far there have been few studies performed that demonstrate teratogenic effects of caffeine on structures of the developing eye, particularly the cornea. The aim of the study was to show ultrastruc...

  17. pH of organ-culture-stored corneas.

    Science.gov (United States)

    Lass, J H; Greiner, J V; Meneses, P; Morgan, D C; Medcalf, S K; Collie, D M; Skelnik, D L; Glonek, T

    1988-10-01

    Changes in intracorneal and storage-medium pH values of organ-culture-stored cat corneas were monitored over a 4-week period. The intracorneal pH was determined using the phosphorus-31 magnetic resonance spectroscopy (31P MRS) chemical shift of inorganic orthophosphate in conjunction with a standard pH titration curve. We incubated 32 adult cat corneas using two similar standard organ-culture methods, one with chondroitin sulfate (method 1) and the other without (method 2). Time-course data at 0, 1, 3 and 4 weeks of storage were used to calculate the rate of pH change. The intracorneal pH was not changed significantly for either organ-culture method; however, the storage-medium pH rate of change declined significantly for both methods (method 1, 0.15 pH units/week; method 2, 0.12 pH units/week). The difference between intracorneal and storage-medium pH values over time increased at a rate of 0.12 and 0.11 pH units/week for method 1 and method 2, respectively. The declining storage-medium pH in conjunction with the maintenance of intracorneal pH contributes to an increased metabolic demand on the cornea. PMID:3218477

  18. Ultrastructural changes in the developing chicken cornea following caffeine administration.

    Directory of Open Access Journals (Sweden)

    Bartel Hieronim

    2010-11-01

    Full Text Available Caffeine is one of the most frequently consumed psychoactive substances. It has been known for many years that caffeine at high concentrations exerts harmful effects on both women's and laboratory animals' fertility, moreover it may impair normal development of many organs in the prenatal period. So far there have been few studies performed that demonstrate teratogenic effects of caffeine on structures of the developing eye, particularly the cornea. The aim of the study was to show ultrastructural changes in the developing cornea, as the effect of caffeine administration to chicken embryos. The experimental materials were 26 chicken embryos from incubated breeding eggs. Eggs were divided into two groups: control (n=30 in which Ringer liquid was administrated, and experimental (n=30 in which teratogenic dose of caffeine 3.5mg/egg was given. In 36th hour of incubation solutions were given with cannula through hole in an egg shell directly onto amniotic membrane. After closing the hole with a glass plate and paraffine, eggs were put back to incubator. In 10th and 19th day of incubation corneas were taken for morphological analysis with a use of electron microscopy. Administration of caffeine during chicken development causes changes of collagen fibers of Bowman's membrane patterns and of the corneal stroma but it also changes proportion of amount of collagen fibers and of the stromal cells.

  19. Stratification of Antigen-presenting Cells within the Normal Cornea

    Directory of Open Access Journals (Sweden)

    Jared E. Knickelbein

    2009-11-01

    Full Text Available The composition and location of professional antigen presenting cells (APC varies in different mucosal surfaces. The cornea, long considered an immune-privileged tissue devoid of APCs, is now known to host a heterogeneous network of bone marrow-derived cells. Here, we utilized transgenic mice that express enhanced green fluorescent protein (EGFP from the CD11c promoter (pCD11c in conjunction with immunohistochemical staining to demonstrate an interesting stratification of APCs within non-inflamed murine corneas. pCD11c+ dendritic cells (DCs reside in the basal epithelium, seemingly embedded in the basement membrane. Most DCs express MHC class II on at least some dendrites, which extend up to 50 µm in length and traverse up 20 µm tangentially towards the apical surface of the epithelium. The DC density diminishes from peripheral to central cornea. Beneath the DCs and adjacent to the stromal side of the basement membrane reside pCD11c-CD11b+ putative macrophages that express low levels of MHC class II. Finally, MHC class IIpCD11c-CD11b+ cells form a network throughout the remainder of the stroma. This highly reproducible stratification of bone marrow-derived cells is suggestive of a progression from an APC function at the exposed corneal surface to an innate immune barrier function deeper in the stroma.

  20. Aberrant Expression and Secretion of Heat Shock Protein 90 in Patients with Bullous Pemphigoid

    Science.gov (United States)

    Tukaj, Stefan; Kleszczyński, Konrad; Vafia, Katerina; Groth, Stephanie; Meyersburg, Damian; Trzonkowski, Piotr; Ludwig, Ralf J.; Zillikens, Detlef; Schmidt, Enno; Fischer, Tobias W.; Kasperkiewicz, Michael

    2013-01-01

    The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP), the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i) Hsp90 was highly expressed in the skin of BP patients, whereas its serum levels were decreased and inversely associated with IgG autoantibody levels against the NC16A immunodominant region of the BP180 autoantigen, (ii) in contrast, neither aberrant levels of circulating Hsp90 nor any correlation of this protein with serum autoantibodies was found in a control cohort of autoimmune bullous disease patients with pemphigus vulgaris, (iii) Hsp90 was highly expressed in and restrictedly released from peripheral blood mononuclear cells of BP patients, and (iv) Hsp90 was potently induced in and restrictedly secreted from human keratinocyte (HaCaT) cells by BP serum and isolated anti-BP180 NC16A IgG autoantibodies, respectively. Our results reveal an upregulated Hsp90 expression at the site of inflammation and an autoantibody-mediated dysregulation of the intracellular and extracellular distribution of this chaperone in BP patients. These findings suggest that Hsp90 may play a pathophysiological role and represent a novel potential treatment target in BP. PMID:23936217

  1. Salt split technique: A useful tool in the diagnosis of subepidermal bullous disorders

    Directory of Open Access Journals (Sweden)

    De Abhishek

    2010-01-01

    Full Text Available Background: Direct immunofluorescence (DIF is the gold standard in the diagnosis of immunobullous diseases. However, it cannot reliably differentiate various subtypes of subepidermal immune- bullous diseases (SIBD. Salt split technique (SST could be used under such circumstances to differentiate them. There is paucity of reports in the Indian literature regarding the SST. Aim: This study was designed to evaluate the utility of direct SST in subepidermal blistering diseases. Materials and Methods: Fourteen clinically diagnosed cases of subepidermal blistering diseases were included in the study. Two perilesional punch biopsies were taken one each for DIF and salt split study. Results: Linear basement membrane zone band with IgG and/or C 3 was seen in 14 cases of patients BP. Salt split study showed epidermal or mixed pattern of deposits in 12 patients and exclusive floor pattern in two patients. The diagnosis was revised in these two patients to epidermolysis bullosa acquisita. Conclusion: SST is a simple, inexpensive procedure and should be routinely employed in the diagnosis of subepidermal bullous diseases.

  2. Heat shock protein 90: a pathophysiological factor and novel treatment target in autoimmune bullous skin diseases.

    Science.gov (United States)

    Tukaj, Stefan; Zillikens, Detlef; Kasperkiewicz, Michael

    2015-08-01

    The chaperone heat shock protein 90 (Hsp90), a cell stress-inducible molecule that regulates activity of many client proteins responsible for cellular growth, differentiation and apoptosis, has been proposed as an important therapeutic target in patients with malignancies. More recently, its active participation in (auto)immune processes has been recognized as evidenced by amelioration of inflammatory disease pathways through pharmacological inhibition of Hsp90 in rodent models of autoimmune encephalomyelitis, rheumatoid arthritis and systemic lupus erythematosus. Based on own current research results, this viewpoint essay provides important insights that Hsp90 is also involved as a notable pathophysiological factor in autoimmune blistering dermatoses including epidermolysis bullosa acquisita, bullous pemphigoid and possibly dermatitis herpetiformis. The observed in vitro, ex vivo and in vivo efficacy of anti-Hsp90 treatment in experimental models of autoimmune bullous diseases and its underlying multimodal anti-inflammatory mechanisms of interference with key contributors to autoimmune-mediated blister formation supports the introduction of selective non-toxic Hsp90 inhibitors into the clinical setting for the treatment of patients with these disorders. PMID:25980533

  3. Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid.

    Directory of Open Access Journals (Sweden)

    Stefan Tukaj

    Full Text Available The cell stress chaperone heat shock protein 90 (Hsp90 has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP, the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i Hsp90 was highly expressed in the skin of BP patients, whereas its serum levels were decreased and inversely associated with IgG autoantibody levels against the NC16A immunodominant region of the BP180 autoantigen, (ii in contrast, neither aberrant levels of circulating Hsp90 nor any correlation of this protein with serum autoantibodies was found in a control cohort of autoimmune bullous disease patients with pemphigus vulgaris, (iii Hsp90 was highly expressed in and restrictedly released from peripheral blood mononuclear cells of BP patients, and (iv Hsp90 was potently induced in and restrictedly secreted from human keratinocyte (HaCaT cells by BP serum and isolated anti-BP180 NC16A IgG autoantibodies, respectively. Our results reveal an upregulated Hsp90 expression at the site of inflammation and an autoantibody-mediated dysregulation of the intracellular and extracellular distribution of this chaperone in BP patients. These findings suggest that Hsp90 may play a pathophysiological role and represent a novel potential treatment target in BP.

  4. Multifocal Bullous Fixed Drug Erruption Due To Phenytoin: A Lesson Learned!

    Science.gov (United States)

    Jain, Ankur; Gupta, Naresh

    2015-12-01

    Antiepileptic drugs (AED) are a common culprit of cutaneous eruptions in clinical practice. Phenytoin, lamotrigine and carbamazepine are the commonest offenders. Maculopapular eruptions are the most frequently reported events. However, multifocal bullous fixed drug eruptions have rarely been described in association with AED use. The risk factors for skin rash including its association with the rate of drug administration are unclear in the literature. We report a case of a young alcoholic man, on long term phenytoin therapy since 3 years, who presented to our emergency department with a breakthrough seizure episode. Patient's routine investigations including serum biochemistry, imaging and toxicology screen were normal. Patient was found to have sub-therapeutic serum phenytoin levels and was prescribed loading with intravenous phenytoin (15mg/kg body weight), which was mistakenly infused at a rapid rate (60mg/minute). Patient developed multifocal bullous lesions over muco-cutaneous regions after 6 hours of drug administration which healed after its discontinuation leaving behind residual hyperpigmentation. Patient was managed conservatively, switched to oral levetiracetam and discharged in a stable condition after one week of hospital stay. Present case highlights a yet uncommon reaction to a commonly used drug and tries to establish the relation between rate of drug infusion and the risk of skin reaction. PMID:26816935

  5. Brittle cornea syndrome: a case report and comparison with Ehlers Danlos syndrome.

    Science.gov (United States)

    Ramappa, Muralidhar; Wilson, M Edward; Rogers, R Curtis; Trivedi, Rupal H

    2014-10-01

    We report a 6-week-old white boy of nonconsanguineous parents who presented with bluish scleral discoloration, thin corneas, and progressive high myopia. A diagnosis of brittle cornea syndrome was confirmed by molecular analysis and prompt measures were taken to manage the condition. Long-term follow-up of children diagnosed with brittle cornea syndrome is important to minimize the risks of corneal rupture and for detecting late-onset systemic conditions. PMID:25266838

  6. Expression of glutathione transferases in corneal cell lines, corneal tissues and a human cornea construct.

    Science.gov (United States)

    Kölln, Christian; Reichl, Stephan

    2016-06-15

    Glutathione transferase (GST) expression and activity were examined in a three-dimensional human cornea construct and were compared to those of excised animal corneas. The objective of this study was to characterize phase II enzyme expression in the cornea construct with respect to its utility as an alternative to animal cornea models. The expression of the GSTO1-1 and GSTP1-1 enzymes was investigated using immunofluorescence staining and western blotting. The level of total glutathione transferase activity was determined using 1-chloro-2,4- dinitrobenzene as the substrate. Furthermore, the levels of GSTO1-1 and GSTP1-1 activity were examined using S-(4-nitrophenacyl)glutathione and ethacrynic acid, respectively, as the specific substrates. The expression and activity levels of these enzymes were examined in the epithelium, stroma and endothelium, the three main cellular layers of the cornea. In summary, the investigated enzymes were detected at both the protein and functional levels in the cornea construct and the excised animal corneas. However, the enzymatic activity levels of the human cornea construct were lower than those of the animal corneas. PMID:27113863

  7. Atomic force microscopy analysis of human cornea surface after UV (λ=266 nm) laser irradiation

    Science.gov (United States)

    Spyratou, E.; Makropoulou, M.; Moutsouris, K.; Bacharis, C.; Serafetinides, A. A.

    2009-07-01

    Efficient cornea reshaping by laser irradiation for correcting refractive errors is still a major issue of interest and study. Although the excimer laser wavelength of 193 nm is generally recognized as successful in ablating corneal tissue for myopia correction, complications in excimer refractive surgery leads to alternative laser sources and methods for efficient cornea treatment. In this work, ablation experiments of human donor cornea flaps were conducted with the 4th harmonic of an Nd:YAG laser, with different laser pulses. AFM analysis was performed for examination of the ablated cornea flap morphology and surface roughness.

  8. Efficacy of combined therapy with tetracycline and nicotinamide for bullous pemphigoid Skuteczność tetracykliny i nikotynamidu w leczeniu pemfigoidu pęcherzowego

    OpenAIRE

    Monika Kałowska; Beata Jakubowska; Katarzyna Woźniak

    2009-01-01

    Introduction: Bullous pemphigoid (BP) is a blistering skin disease which usually affects older individuals, who infrequently have different medical problems. Aim of the study: Evaluation of the efficacy of tetracycline 1.5 g/d with nicotynamide 0.6 g/d and topical 0.05% clobetasol cream in four female patients with bullous pemphigoid. Results: In all four female patients cessation of blister and urticarial lesion formation was noticed within 7-12 days, whereas reepithelization finished within...

  9. Definitions and outcome measures for bullous pemphigoid: Recommendations by an international panel of experts

    Science.gov (United States)

    Murrell, Dedee F.; Daniel, Benjamin S.; Joly, Pascal; Borradori, Luca; Amagai, Masayuki; Hashimoto, Takashi; Caux, Frédéric; Marinovic, Branka; Sinha, Animesh A.; Hertl, Michael; Bernard, Philippe; Sirois, David; Cianchini, Giuseppe; Fairley, Janet A.; Jonkman, Marcel F.; Pandya, Amit G.; Rubenstein, David; Zillikens, Detlef; Payne, Aimee S.; Woodley, David; Zambruno, Giovanna; Aoki, Valeria; Pincelli, Carlo; Diaz, Luis; Hall, Russell P.; Meurer, Michael; Mascaro, Jose M.; Schmidt, Enno; Shimizu, Hiroshi; Zone, John; Swerlick, Robert; Mimouni, Daniel; Culton, Donna; Lipozencic, Jasna; Bince, Benjamin; Grando, Sergei A.; Bystryn, Jean-Claude; Werth, Victoria P.

    2011-01-01

    Our scientific knowledge of bullous pemphigoid (BP) has dramatically progressed in recent years. However, despite the availability of various therapeutic options for the treatment of inflammatory diseases, only a few multicenter controlled trials have helped to define effective therapies in BP. A major obstacle in sharing multicenter-based evidences for therapeutic efforts is the lack of generally accepted definitions for the clinical evaluation of patients with BP. Common terms and end points of BP are needed so that experts in the field can accurately measure and assess disease extent, activity, severity, and therapeutic response, and thus facilitate and advance clinical trials. These recommendations from the International Pemphigoid Committee represent 2 years of collaborative efforts to attain mutually acceptable common definitions for BP and proposes a disease extent score, the BP Disease Area Index. These items should assist in the development of consistent reporting of outcomes in future BP reports and studies. PMID:22056920

  10. Development of bullous pemphigoid during treatment of psoriatic onycho-pachydermo periostitis with ustekinumab.

    Science.gov (United States)

    Nakayama, Chihiro; Fujita, Yasuyuki; Watanabe, Mika; Shimizu, Hiroshi

    2015-10-01

    Ustekinumab is a human monoclonal antibody that specifically binds to the p40 subunit of interleukin (IL)-12 and IL-23, inhibiting the activity of both cytokines, thereby blocking the T-helper (Th)1 and Th17 inflammatory pathways. While biologic agents have dramatically changed the strategies of psoriasis treatment, increasing cases of autoimmune diseases during the use of such agents have been reported. We experienced a case of bullous pemphigoid occurring during treatment of a rare variant of psoriatic arthritis, psoriatic onycho-pachydermo periostitis with ustekinumab. Only six cases of autoimmune blistering diseases during treatment with biologic agents have ever been reported including our case, and we herein review the published work of these cases. Dermatologists must be attentive to the possibility of autoimmune blistering diseases during ustekinumab treatment. PMID:26010891

  11. Development of a disease registry for autoimmune bullous diseases: initial analysis of the pemphigus vulgaris subset.

    Science.gov (United States)

    Shah, Amit Aakash; Seiffert-Sinha, Kristina; Sirois, David; Werth, Victoria P; Rengarajan, Badri; Zrnchik, William; Attwood, Kristopher; Sinha, Animesh A

    2015-01-01

    Pemphigus vulgaris (PV) is a rare, potentially life threatening, autoimmune blistering skin disease. The International Pemphigus and Pemphigoid Foundation (IPPF) has recently developed a disease registry with the aim to enhance our understanding of autoimmune bullous diseases with the long-term goal of acquiring information to improve patient care. Patients were recruited to the IPPF disease registry through direct mail, e-mail, advertisements, and articles in the IPPF-quarterly, -website, -Facebook webpage, and IPPF Peer Health Coaches to complete a 38-question survey. We present here the initial analysis of detailed clinical information collected on 393 PV patients. We report previously unrecognized gender differences in terms of lesion location, autoimmune comorbidity, and delay in diagnosis. The IPPF disease registry serves as a useful resource and guide for future clinical investigation. PMID:24691863

  12. A Retrospective and Comparative Study of Familial and Non-familial Bullous Lichen Planus

    Institute of Scientific and Technical Information of China (English)

    HUANG Changzheng; YAN Xiaofeng; YANG Lingyun; ZHANG Jing; TIAN Jin; LI Jiawen; WANG Chunsen; TU Yating

    2007-01-01

    In order to compare the clinical characteristics of familial and non-familial bullous lichen planus (BLP), the archival data of 36 BLP patients with positive family history and 21 BLP patients with negative family history diagnosed according to the clinical features and histopathology were collected in our department from 1956 to 2003. The clinical features were analyzed and compared. There were significant differences between familial and non-familial BLP in age of onset, duration of disease and extension of eruption (P<0.01). It was concluded that familial BLP appeared to differ from the non-familial form in its earlier age of onset, longer duration of the disease, more extensive eruption and more tendency to involve nails. Hereditary factors may play a role in the pathogenesis of familial BLP.

  13. Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid.

    Science.gov (United States)

    Mersmann, Michael; Dworschak, Jenny; Ebermann, Kristin; Komorowski, Lars; Schlumberger, Wolfgang; Stöcker, Winfried; Zillikens, Detlef; Probst, Christian; Schmidt, Enno

    2016-01-01

    Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity. PMID:26498290

  14. Validation of tissue quality parameters for donor corneas, designated for emergency cases: corneal graft survival

    NARCIS (Netherlands)

    W.J. Rijneveld; R. Wolff; H.J.M. Volker-Dieben; E. Pels

    2011-01-01

    Purpose: To validate tissue quality parameters for donor corneas designated for emergency grafting for corneal graft survival. Methods: In a longitudinal cohort follow-up study, 131 emergency penetrating grafts were studied. Grafts were performed with a pool of organ-cultured donor corneas designate

  15. Xanthelasmata, arcus corneae, and ischaemic vascular disease and death in general population: prospective cohort study

    DEFF Research Database (Denmark)

    Christoffersen, Mette; Frikke-Schmidt, Ruth; Schnohr, Peter; Jensen, Gorm B; Nordestgaard, Børge G; Tybjærg-Hansen, Anne

    2011-01-01

    To test the hypothesis that xanthelasmata and arcus corneae, individually and combined, predict risk of ischaemic vascular disease and death in the general population.......To test the hypothesis that xanthelasmata and arcus corneae, individually and combined, predict risk of ischaemic vascular disease and death in the general population....

  16. Use of Fourier-Domain Optical Coherence Tomography to Evaluate Anterior Stromal Opacities in Donor Corneas

    Directory of Open Access Journals (Sweden)

    Matthew R. Bald

    2013-01-01

    Full Text Available Purpose. To evaluate Fourier-domain optical coherence tomography (FD-OCT as an adjunct to traditional slit lamp examination of donor corneas with suspected Anterior Stromal Opacities. Methods. Seven corneas suspected of having anterior stromal opacities by slit lamp examination were evaluated with FD-OCT. Each cornea was evaluated to confirm the presence of opacity and, if present, the depth of opacity was measured. Results. The opacity depth ranged from 82 μm to 624 μm. The initial slit lamp impressions of five of the seven corneas were confirmed by OCT. In two corneas, the OCT findings were different from the initial slit lamp impressions. Slit lamp examination of the first cornea gave the impression of anterior stromal scarring, but OCT showed that the opacity was limited to the epithelium. Slit lamp examination of the second cornea suggested opacity limited to the epithelium, but OCT identified significant sub-Bowman's scarring. In all cases, the Eye Bank Technicians reported that the location and depth of corneal opacity were more sharply defined by OCT than by slit lamp. Conclusion. The high resolution of OCT makes it easier to determine the location of corneal opacities compared to slit lamp examinations. This enhanced visualization can improve decisions regarding transplant suitability of donor corneas.

  17. Corneal decompensation following filtering surgery with the Ex-PRESS® mini glaucoma shunt device

    Directory of Open Access Journals (Sweden)

    Tojo N

    2015-03-01

    Full Text Available Naoki Tojo, Atsushi Hayashi, Akio Miyakoshi Department of Ophthalmology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan Purpose: To report a case of corneal decompensation due to the Ex-PRESS® mini glaucoma shunt device (Ex-PRESS.Patient and methods: A 75-year-old man had pseudoexfoliation glaucoma in his right eye. He underwent filtration surgery with Ex-PRESS. His intraocular pressure was 7 mmHg after 9 months.Results: We observed partial decompensation of the corneal endothelium adjacent to the filtering bleb. Specular microscopy revealed a marked decrease in the endothelial cell density at the center of the cornea.Conclusion: Anterior segment optical coherence tomography is very useful for evaluating corneal edema and the position of Ex-PRESS. It is important to follow up with an examination of the corneal endothelial cells. Keywords: Ex-PRESS, bullous keratopathy, trabeculectomy, complication, cornea 

  18. Identification of the cutaneous basement membrane zone antigen and isolation of antibody in linear immunoglobulin A bullous dermatosis.

    OpenAIRE

    Zone, J J; Taylor, T B; Kadunce, D P; Meyer, L J

    1990-01-01

    Linear IgA bullous dermatosis (LABD) is a rare blistering skin disease characterized by basement membrane zone deposition of IgA. This study identifies a tissue antigen detected by patient serum and then isolates the autoantibody using epidermis and protein bands blotted on nitrocellulose as immunoabsorbents. Sera from 10 patients (9 with cutaneous disease and 1 with cicatrizing conjunctivitis) were evaluated. Indirect immunofluorescence revealed an IgA anti-basement membrane antibody in 6 of...

  19. Combined acute interstitial pneumonitis and pancytopenia induced by low-dose methotrexate in a hemodialysis patient treated for bullous pemphigoid*

    OpenAIRE

    Liu, Haibo; Liu, Fang; Zhang, Min; Yan, Wenliang; Sang, Hong

    2015-01-01

    Methotrexate has been widely used for many years in the treatment of a variety of diseases. Acute pneumonitis and bone marrow suppression are very serious side effects in methotrexate treatment. A 48-year-old man with end-stage renal disease undergoing chronic hemodialysis developed combined acute pneumonitis and pancytopenia after a cumulative dose of 20 mg methotrexate for bullous pemphigoid. Continuous renal replacement therapy (CRRT) can effi ciently decrease serum methotrexate concentrat...

  20. Imaging of Keratoconic and normal human cornea with a Brillouin imaging system (Conference Presentation)

    Science.gov (United States)

    Besner, Sebastien; Shao, Peng; Scarcelli, Giuliano; Pineda, Roberto; Yun, Seok-Hyun (Andy)

    2016-03-01

    Keratoconus is a degenerative disorder of the eye characterized by human cornea thinning and morphological change to a more conical shape. Current diagnosis of this disease relies on topographic imaging of the cornea. Early and differential diagnosis is difficult. In keratoconus, mechanical properties are found to be compromised. A clinically available invasive technique capable of measuring the mechanical properties of the cornea is of significant importance for understanding the mechanism of keratoconus development and improve detection and intervention in keratoconus. The capability of Brillouin imaging to detect local longitudinal modulus in human cornea has been demonstrated previously. We report our non-contact, non-invasive, clinically viable Brillouin imaging system engineered to evaluate mechanical properties human cornea in vivo. The system takes advantage of a highly dispersive 2-stage virtually imaged phased array (VIPA) to detect weak Brillouin scattering signal from biological samples. With a 1.5-mW light beam from a 780-nm single-wavelength laser source, the system is able to detect Brillouin frequency shift of a single point in human cornea less than 0.3 second, at a 5μm/30μm lateral/axial resolution. Sensitivity of the system was quantified to be ~ 10 MHz. A-scans at different sample locations on a human cornea with a motorized human interface. We imaged both normal and keratoconic human corneas with this system. Whereas no significantly difference were observed outside keratocnic cones compared with normal cornea, a highly statistically significantly decrease was found in the cone regions.

  1. Correction of eye refraction by nonablative laser action on thermomechanical properties of cornea and sclera

    International Nuclear Information System (INIS)

    A new approach is proposed for correcting the eye refraction by controlled variation of the mechanical properties of the sclera and cornea upon nondestructive laser heating. Experimental ex vivo studies of rabbit and pig eyes show that laser-induced local denaturation of the sclera changes the refraction of the cornea by 3 diopters on the average, and the subsequent nondestructive irradiation of the cornea increases its plasticity, which leads to a further increase in its radius of curvature and a decrease in refraction down to 7 diopters.

  2. A time-delay calibrated method for cornea hysteresis and intraocular pressure measurement

    Science.gov (United States)

    Wang, Kuo-Jen; Tsai, Che-Liang; Wang, Wai; Hsu, Long; Hsu, Ken-Yuh

    2016-04-01

    The presence of cornea hysteresis (CH) in characterizing the intraocular pressure (IOP) of a human eye deteriorates the accuracy of IOP. To suppress CH, the pressure gauge of a tonometer must be located as close as possible to the cornea. However, this arrangement is unpractical because appropriate working distance to the cornea is required. In this paper, a time-delay calibrated (TDC) method is proposed to counteract the undesired effect of CH in characterizing the IOP. Employing this TDC method, the CH approaches to zero for most eyes measured.

  3. Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model.

    Science.gov (United States)

    Hashimoto, Yoshihide; Hattori, Shinya; Sasaki, Shuji; Honda, Takako; Kimura, Tsuyoshi; Funamoto, Seiichi; Kobayashi, Hisatoshi; Kishida, Akio

    2016-01-01

    The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on the decellularization of the corneal ultrastructure and its involvement in transparency, and assessed the in vivo behaviour of the decellularized cornea using two animal transplantation models, in relation to remodelling of collagen fibrils. Decellularized corneas were prepared by the HHP method. The decellularized corneas were executed by haematoxylin and eosin and Masson's trichrome staining to demonstrate the complete removal of corneal cells. Transmission electron microscopy revealed that the ultrastructure of the decellularized cornea prepared by the HHP method was better maintained than that of the decellularized cornea prepared by the detergent method. The decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty using a rabbit model was stable and remained transparent without ultrastructural alterations. We conclude that the superior properties of the decellularized cornea prepared by the HHP method were attributed to the preservation of the corneal ultrastructure. PMID:27291975

  4. Exploring mechanisms of IgE-mediated autoimmunity through the lens of bullous pemphigoid.

    Science.gov (United States)

    Messingham, Kelly N; Randall, Grant; Fairley, Janet

    2016-04-01

    Bullous pemphigoid (BP) is the most common autoimmune blistering disease characterized by pathogenic autoantibodies targeting collagen XVII (col XVII), a hemidesmosomal adhesion molecule. Early studies utilizing IgG were critical for establishing col XVII-specific antibodies as primary mediators of blister formation; however, these studies lacked key features of the disease, including urticarial erythema and eosinophilic infiltration, which are often associated with IgE. Although it was recognized that BP patients often had elevated circulating IgE, investigations into the pathogenicity of these antibodies was delayed until discovery of col XVII-specific IgE in BP sera. Since then, a variety of in-vivo and in-vitro studies have provided clear evidence that IgE autoantibodies are a key component of BP. Furthermore, studies utilizing IgE receptor blockade in BP patients were the first to confirm a pathogenic role of IgE autoantibodies in human autoimmunity. In this review we will utilize BP as a prototypical autoimmune disease to better understand how IgE autoantibodies participate in human autoimmunity. PMID:26959553

  5. Mastocitose bolhosa na criança: relato de caso Bullous mastocytosis in child: case report

    Directory of Open Access Journals (Sweden)

    Ana Lúcia França da Costa

    2005-12-01

    Full Text Available A mastocitose é doença rara, definida como a infiltração desordenada de mastócitos em vários tecidos. Compreende diferentes quadros clínicos, variando de formas cutâneas indolentes a condições sistêmicas e malignas. O termo mastocitose bolhosa descreve a variedade na qual a lesão cutânea bolhosa é o aspecto predominante e geralmente indica casos de mastocitose cutânea. É mais comum na criança e possui prognóstico mais reservado. Este relato de caso enfatiza a diversidade de suas manifestações clínicas e as complicações decorrentes.Mastocytosis is a rare disease, defined as a disorderly infiltration with mast cells in several tissues. It comprises many different clinical situations varying from indolent cutaneous forms to malignant and systemic conditions. The term bullous mastocytosis describes all the variety in which the cutaneous bullae represent the predominant feature and generally indicates cases of diffuse cutaneous mastocytosis. Its presence is more common during the childhood and has a more reserved prognosis. This case report emphasizes the extreme diversity of its clinical manifestations and complications.

  6. Treatment of Intraepidermal Autoimmune Bullous Diseases Sürekli Eğitim

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    Tamer İrfan Kaya

    2011-06-01

    Full Text Available Pemfigus is an autoimmune bullous skin disease, characterized by intraepidermal blisters. It is a severe and potentially life-threatening chronic disease with blisters and erosions on the mucosae and the skin. Treatment options do not differ for two most common types of pemphigus, pemphigus vulgaris and pemphigus foliaceus, except that the latter is usually less resistant to treatment and corticosteroids can often be started at lower doses. Systemic corticosteroids are still the most widely used drugs in the treatment of pemphigus and continue to be the mainstay of therapy for this disease. Adjuvant drugs are commonly used in combination with the aims of increasing efficacy and of having a steroid-sparing action, thereby allowing reduced corticosteroid side-effects. Mortality and complete remission rates have improved since the introduction of adjuvant drugs to pemphigus. Adjuvant drugs include immunoadsorbtion, corticosteroid pulse therapy, intravenous immunoglobulin (IVIG, immunosuppressive agents such as azathioprine, cyclophosphamide, mycophenolate mofetil and and anti-CD20 monoclonal antibody (rituximab. The lack of consensus in the published literature about the treatment of this disorder is responsible for different treatment strategies. Treatments need to be chosen after careful consideration of the potential benefits and side effects according to the patients’ medical condition. Here, both conventional therapies and novel treatment regimens for pemphigus are discussed. (Turkderm 2011; 45 Suppl 1: 44-53

  7. The filtering, clear-cornea diathermal keratostomy: a minor Danish multicenter study

    DEFF Research Database (Denmark)

    Kessing, S.V.; Nissen, O.I.; Thygesen, J.; Flesner, P.; Otland, N.; Riise, P.

    2008-01-01

    PURPOSE: Is the new micropenetrating, clear-cornea procedure, intrastromal diathermal keratostomy (IDK), an alternative to the intricate "modern trabeculectomy"? METHODS: Prospective multicenter study. Four surgeons from 4 Danish eye departments attended an IDK course and subsequently decided whe...

  8. A role for smoothened during murine lens and cornea development.

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    Janet J Y Choi

    Full Text Available Various studies suggest that Hedgehog (Hh signalling plays roles in human and zebrafish ocular development. Recent studies (Kerr et al., Invest Ophthalmol Vis Sci. 2012; 53, 3316-30 showed that conditionally activating Hh signals promotes murine lens epithelial cell proliferation and disrupts fibre differentiation. In this study we examined the expression of the Hh pathway and the requirement for the Smoothened gene in murine lens development. Expression of Hh pathway components in developing lens was examined by RT-PCR, immunofluorescence and in situ hybridisation. The requirement of Smo in lens development was determined by conditional loss-of-function mutations, using LeCre and MLR10 Cre transgenic mice. The phenotype of mutant mice was examined by immunofluorescence for various markers of cell cycle, lens and cornea differentiation. Hh pathway components (Ptch1, Smo, Gli2, Gli3 were detected in lens epithelium from E12.5. Gli2 was particularly localised to mitotic nuclei and, at E13.5, Gli3 exhibited a shift from cytosol to nucleus, suggesting distinct roles for these transcription factors. Conditional deletion of Smo, from ∼E12.5 (MLR10 Cre did not affect ocular development, whereas deletion from ∼E9.5 (LeCre resulted in lens and corneal defects from E14.5. Mutant lenses were smaller and showed normal expression of p57Kip2, c-Maf, E-cadherin and Pax6, reduced expression of FoxE3 and Ptch1 and decreased nuclear Hes1. There was normal G1-S phase but decreased G2-M phase transition at E16.5 and epithelial cell death from E14.5-E16.5. Mutant corneas were thicker due to aberrant migration of Nrp2+ cells from the extraocular mesenchyme, resulting in delayed corneal endothelial but normal epithelial differentiation. These results indicate the Hh pathway is required during a discrete period (E9.5-E12.5 in lens development to regulate lens epithelial cell proliferation, survival and FoxE3 expression. Defective corneal development occurs

  9. Cost Minimization Analysis of Precut Cornea Grafts in Descemet Stripping Automated Endothelial Keratoplasty.

    Science.gov (United States)

    Yong, Kai-Ling; Nguyen, Hai V; Cajucom-Uy, Howard Y; Foo, Valencia; Tan, Donald; Finkelstein, Eric A; Mehta, Jodhbir S

    2016-02-01

    Descemet stripping automated endothelial keratoplasty (DSAEK) is the most common corneal transplant procedure. A key step in the procedure is preparing the donor cornea for transplantation. This can be accomplished via 1 of 3 alternatives: surgeon cuts the cornea on the day of surgery, the cornea is precut ahead of time in an offsite facility by a trained technician, or a precut cornea is purchased from an eye bank. Currently, there is little evidence on the costs and effectiveness of these 3 strategies to allow healthcare providers decide upon the preferred method to prepare grafts.The aim of this study was to compare the costs and relative effectiveness of each strategy.The Singapore National Eye Centre and Singapore Eye Bank performed both precut cornea and surgeon-cut cornea transplant services between 2009 and 2013.This study included 110 subjects who received precut cornea and 140 who received surgeon-cut cornea. Clinical outcomes and surgical duration were compared across the strategies using the propensity score matching. The cost of each strategy was estimated using the microcosting and consisted of facility costs and procedural costs including surgical duration. One-way sensitivity analysis and threshold analysis were performed.The cost for DSAEK was highest for the surgeon-cut approach ($13,965 per procedure), followed by purchasing precut corneas ($12,659) and then setting up precutting ($12,421). The higher procedural cost of the surgeon-cut approach was largely due to the longer duration of the procedure (surgeon-cut = 72.54 minutes, precut = 59.45 minutes, P cut. Threshold analysis demonstrated that if the number of cases was below 31 a year, the strategy that yielded the lowest cost was purchasing precut cornea from eye bank. If there were more than 290 cases annually, the cheapest option would be to setup precutting facility.Our findings suggest that it is more efficient for centers that are performing a large number of cornea

  10. Hyperglycemia-induced abnormalities in rat and human corneas: the potential of second harmonic generation microscopy.

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    Gaël Latour

    Full Text Available BACKGROUND: Second Harmonic Generation (SHG microscopy recently appeared as an efficient optical imaging technique to probe unstained collagen-rich tissues like cornea. Moreover, corneal remodeling occurs in many diseases and precise characterization requires overcoming the limitations of conventional techniques. In this work, we focus on diabetes, which affects hundreds of million people worldwide and most often leads to diabetic retinopathy, with no early diagnostic tool. This study then aims to establish the potential of SHG microscopy for in situ detection and characterization of hyperglycemia-induced abnormalities in the Descemet's membrane, in the posterior cornea. METHODOLOGY/PRINCIPAL FINDINGS: We studied corneas from age-matched control and Goto-Kakizaki rats, a spontaneous model of type 2 diabetes, and corneas from human donors with type 2 diabetes and without any diabetes. SHG imaging was compared to confocal microscopy, to histology characterization using conventional staining and transmitted light microscopy and to transmission electron microscopy. SHG imaging revealed collagen deposits in the Descemet's membrane of unstained corneas in a unique way compared to these gold standard techniques in ophthalmology. It provided background-free images of the three-dimensional interwoven distribution of the collagen deposits, with improved contrast compared to confocal microscopy. It also provided structural capability in intact corneas because of its high specificity to fibrillar collagen, with substantially larger field of view than transmission electron microscopy. Moreover, in vivo SHG imaging was demonstrated in Goto-Kakizaki rats. CONCLUSIONS/SIGNIFICANCE: Our study shows unambiguously the high potential of SHG microscopy for three-dimensional characterization of structural abnormalities in unstained corneas. Furthermore, our demonstration of in vivo SHG imaging opens the way to long-term dynamical studies. This method should be easily

  11. Taurine effect on cytogenetic lesions in the cornea of mice exposed to 9 Gev proton irradiation

    International Nuclear Information System (INIS)

    Possibilities of preventive measures and treatment of cytogenetic injuries in the mice cornea, subjected to proton irradiation at 9 Gev were studied. Taurine containing solution (TCS) was used as a radiomodifying agent. It is shown that TCS application enables to decrease aberrant mitoses level in cornea epithelium cells of mice. Antiactinic effect of the above agent is determined by its considerable action on mitotic delay

  12. Diabetic cornea wounds produce significantly weaker electric signals that may contribute to impaired healing

    OpenAIRE

    Yunyun Shen; Trisha Pfluger; Fernando Ferreira; Jiebing Liang; Navedo, Manuel F.; Qunli Zeng; Brian Reid; Min Zhao

    2016-01-01

    Wounds naturally produce electric signals which serve as powerful cues that stimulate and guide cell migration during wound healing. In diabetic patients, impaired wound healing is one of the most challenging complications in diabetes management. A fundamental gap in knowledge is whether diabetic wounds have abnormal electric signaling. Here we used a vibrating probe to demonstrate that diabetic corneas produced significantly weaker wound electric signals than the normal cornea. This was conf...

  13. [The value of methods for morphofunctional analysis of cornea in cataract surgery].

    Science.gov (United States)

    Borodina, N B; Kobzova, M V; Musaeva, G M

    2011-01-01

    The characteristics of morphofunctional status of cornea after extracapsular cataract extraction and phakoemulsification with IOL implantation (30 and 58 operations respectively) were analyzed in detail using up-to-date diagnostic techniques. The results of examination using developed algorithm including study of light transmission, refraction and protective function of cornea show advantage of microinvasive ultrasound technique of cataract surgery in terms of minimal impact on corneal structure, optical and biomechanical characteristics. PMID:22165096

  14. Screening human donor corneas during organ culture for the presence of guttae

    OpenAIRE

    Borderie, V; Sabolic, V.; Touzeau, O.; Scheer, S; Carvajal-Gonzalez, S.; Laroche, L.

    2001-01-01

    AIMS—To detect the presence of guttae by means of light microscopy during organ culture and to evaluate the influence of the presence of guttae in the donor tissue on transplantation outcome.
METHODS—Donor corneas were investigated for the presence of guttae by means of light microscopy at the end of organ culture. Recipient corneal buttons from patients with severe Fuchs' dystrophy and donor corneas with advanced guttae were first studied by light microscopy and subsequently by transmission ...

  15. Intrastromal Injection of China Painting Ink in Corneas of Male Rabbits: Clinical and Histological Study.

    Science.gov (United States)

    Alsmman Hassan, Alahmady Hamad; Abd Elhaliem Soliman, Nesreen Gamal-Eldeen

    2016-01-01

    Background. Many patients with corneal opacity or complicated cataract in blind eye ask for cosmoses. In this study we tried to investigate the staining of corneas of male rabbits by Rotring China painting ink and to study the histological changes. Method. 10 eyes of 10 male Baladi Egyptian rabbits were injected (0.1 mL) intrastromally in the cornea by the use of China painting ink (Rotring Tinta China) through insulin syringe (27-gauge needle) by single injection; clinical follow-up is for 6 months and lastly the rabbits were scarified and the stained eyes were enucleated for histological analysis. Results. Clinically the stain was stable in color and distribution in corneas with no major complications. Histological results of the stained rabbit corneas showed blackish pigmentation in the corneal stroma without any inflammatory cellular infiltration. Some fibroblast cells had pigment granules in their cytoplasm in the adjacent layers. Conclusion. Corneal staining by China painting ink is effective and safe in staining of male rabbits cornea; however further study in human corneas with longer follow-up period is advisable. PMID:27195146

  16. Intrastromal Injection of China Painting Ink in Corneas of Male Rabbits: Clinical and Histological Study

    Directory of Open Access Journals (Sweden)

    Alahmady Hamad Alsmman Hassan

    2016-01-01

    Full Text Available Background. Many patients with corneal opacity or complicated cataract in blind eye ask for cosmoses. In this study we tried to investigate the staining of corneas of male rabbits by Rotring China painting ink and to study the histological changes. Method. 10 eyes of 10 male Baladi Egyptian rabbits were injected (0.1 mL intrastromally in the cornea by the use of China painting ink (Rotring Tinta China through insulin syringe (27-gauge needle by single injection; clinical follow-up is for 6 months and lastly the rabbits were scarified and the stained eyes were enucleated for histological analysis. Results. Clinically the stain was stable in color and distribution in corneas with no major complications. Histological results of the stained rabbit corneas showed blackish pigmentation in the corneal stroma without any inflammatory cellular infiltration. Some fibroblast cells had pigment granules in their cytoplasm in the adjacent layers. Conclusion. Corneal staining by China painting ink is effective and safe in staining of male rabbits cornea; however further study in human corneas with longer follow-up period is advisable.

  17. Use of Fish Scale-Derived BioCornea to Seal Full-Thickness Corneal Perforations in Pig Models.

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    Shih-Cheng Chen

    Full Text Available The aim of this study was to test the use of BioCornea, a fish scale-derived collagen matrix for sealing full-thickness corneal perforations in mini-pigs. Two series of experiments were carried out in 8 Lan-Yu and 3 Göttingen mini-pigs, respectively. A 2mm central full thickness corneal perforation was made with surgical scissors and 2mm trephines. The perforations were sealed immediately by suturing BioCornea to the wounded cornea. The conditions of each patched cornea were followed-up daily for 3 or 4 days. Status of operated eyes was assessed with slit lamp examination or optical coherence tomography (OCT. Animals were sacrificed after the study period and the corneas operated were fixated for histological examination. Both OCT imaging and handheld slit lamp observations indicated that a stable ocular integrity of the perforated corneas was maintained, showing no leakage of aqueous humor, normal depth of anterior chamber and only mild swelling of the wounded cornea. Hematoxylin and eosin staining of the patched cornea showed no epithelial ingrowths to the perforated wounds and no severe leucocyte infiltration of the stroma. The fish scale-derived BioCornea is capable to seal full-thickness corneal perforation and stabilize the integrity of ocular anterior chamber in pre-clinic mini-pig models. BioCornea seems to be a safe and effective alternative for emergency treatment of corneal perforations.

  18. Polymicrobial Infection of the Cornea Due to Contact Lens Wear

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    Selçuk Sızmaz

    2016-04-01

    Full Text Available A 38-year-old male presented with pain and redness in his left eye. He had a history of wearing contact lenses. His ophthalmic examination revealed a large corneal ulcer with surrounding infiltrate. Cultures were isolated from the contact lenses, lens solutions, storage cases, and conjunctivae of both eyes and also corneal scrapings of the left eye. Fortified vancomycin and amikacin drops were started hourly. Culture results of conjunctivae of each eye and left cornea were positive for Pseudomonas aeruginosa; cultures from the contact lenses, lens solution and storage case of both eyes revealed Pseudomonas aeruginosa and Alcaligenes xylosoxidans. Polymerase chain reaction of the corneal scraping was positive for Acanthameoba. The topical antibiotics were changed with ones that both bacteria were sensitive to and anti-amoebic therapy was added. The patient had two recurrences following initial presentation despite intensive therapy. Keratitis occurred due to multiple pathogens; the relapsing course despite adequate therapy is potentially associated with this polymicrobial etiology.

  19. Collagen cross-linking: Strengthening the unstable cornea

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    Oren Tomkins

    2008-05-01

    Full Text Available Oren Tomkins, Hanna J GarzoziDepartment of Ophthalmology, Bnai Zion Medical Center, Haifa, IsraelAbstract: Corneal ectasia, a weakening of corneal integrity, occurs both due to acquired and congenital conditions such as keratoconus. It is a progressing condition that affects both visual acuity, and corneal stability. Various methods exist for correcting this impairment, however none address the inherit pathology, an increase laxity of the corneal stroma. Collagen crosslinking, a new, minimally invasive method, aims to strengthen the stroma by inducing cross links between neighboring collagen fibers. This method results in an increase in corneal tensile strength, with no medium term adverse effects on its normal architecture. Clinically, treated patients display improvement in both visual acuity and keratometric readings. This method may provide clinicians with easily accessible tools to stop the progression, and even correct visual deterioration due to corneal ectasia. Here we review the current information regarding this new method, as well as discuss its potential benefits and downfalls.Keywords: corneal cross-linking, corneal ectasia, keratoconus, stroma, cornea

  20. Temperature control during diode laser welding in a human cornea

    Science.gov (United States)

    Rossi, Francesca; Matteini, Paolo; Pini, Roberto; Menabuoni, Luca

    2007-07-01

    Diode laser welding is a technique proposed in ophthalmic surgery to induce immediate sealing of clear corneal wounds. The welding effect is achieved irradiating the area, previously treated with a chromophore, by the use of a low power diode laser: the resulting thermal effect induces structural modifications in the stromal collagen, that welds upon cooling. We present a study on the temperature dynamics developing during welding in a human eye. An infrared thermocamera was used to measure the temperature variations on the surface of the cornea during clinical penetrating keratoplasty (corneal transplant). The experimental data were used as a starting point for a theoretical investigation of the temperature rising inside the ocular structures: we developed a mathematical model based on the bio-heat equation and solved by the use of the Finite Element Method (FEM). The predictive accuracy was verified by comparing the temperature post-processing description with the results obtained from the thermographic data. The model was then used to study the temperature rise and heat propagation inside the eye. Experimental results and model analysis indicated the occurrence of heat confinement during the treatment procedure and a modest enhancement of the temperature (reaching about 55°C inside the laser treated wound), thus evidencing the safety of the procedure in clinical applications.

  1. Possible paraneoplastic syndrome case of bullous pemphigoid with immunoglobulin G anti-BP180 C-terminal domain antibodies associated with psoriasis and primary macroglobulinemia.

    Science.gov (United States)

    Maki, Nobuki; Demitsu, Toshio; Umemoto, Naoka; Nagashima, Kazutaka; Nakamura, Toshinobu; Kakurai, Maki; Nakamura, Satoshi; Yamada, Tomoko; Ishii, Norito; Hashimoto, Takashi

    2016-05-01

    A 61-year-old Japanese man developed bullous skin lesions during topical therapy for psoriasis vulgaris. Physical examination demonstrated numerous tense bullae and scaly erythemas on the trunk and extremities. Histopathology of the skin biopsy demonstrated subepidermal bullae and lymphocytic infiltration with eosinophils in the dermis. Direct immunofluorescence revealed linear deposits of immunoglobulin (Ig)G, IgA and C3 along the basement membrane zone. Indirect immunofluorescence of 1 mol/L NaCl-split skin showed IgG reactivity with both epidermal and the dermal sides. IgM reactivity with both the epidermal and dermal sides was also detected. Enzyme-linked immunosorbent assays showed negative results for both BP180 and BP230. Immunoelectrophoresis of serum and bone marrow aspiration revealed underlying primary macroglobulinemia with M-proteinemia of IgM-κ type. Immunoblot analysis revealed IgG, but not IgM, antibodies to recombinant protein of BP180 C-terminal domain. We diagnosed the present case as bullous pemphigoid with IgG anti-BP180 C-terminal domain autoantibodies associated with primary macroglobulinemia and psoriasis vulgaris. Systemic administration of prednisolone 30 mg/day resulted in dramatic improvement of both bullous and psoriatic skin lesions. When the bullous and psoriatic lesions relapsed, DRC chemotherapy (dexamethasone, rituximab and cyclophosphamide) for macroglobulinemia was performed. Then, the psoriatic lesions improved and the bullous lesions disappeared. We suggested that the present case may be paraneoplastic syndrome of bullous pemphigoid associated with primary macroglobulinemia and psoriasis vulgaris. PMID:26507447

  2. A 14-year paraneoplastic rash: urticarial vasculitis and dermal binding bullous pemphigoid secondary to chronic lymphocytic leukaemia.

    Science.gov (United States)

    Kassim, J M; Igali, L; Levell, N J

    2015-06-01

    A 72-year-old woman with a 14-year history of urticarial vasculitis (UV) and a 13-year history of bullous pemphigoid (BP) presented with associated progressive chronic lymphocytic leukaemia (CLL). Both skin conditions responded poorly to treatment, until chemotherapy for CLL was commenced. The skin features showed a clear paraneoplastic course, resolving with chemotherapy and recurring when the CLL relapsed and the lymphocyte count rose above 5 × 10(9)/L. No case of UV secondary to CLL, and very few cases of BP related to CLL have been reported, and no paraneoplastic rash of any type lasting 14 years has been reported previously. PMID:25524180

  3. Three-year observation of the patients with bullous pemphigoid: 29 cases

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    Özlem Ekiz

    2013-12-01

    Full Text Available Background and Design: Bullous pemphigoid (BP is a chronic, autoimmune, subepidermal blistering disease. The aim of this study was to determine the clinico-epidemiologic data’s of patients diagnosed as BP in our clinic and compare these data with other studies in our country and in the world. Material and Method: We researched the documents of BP cases diagnosed by clinical and histopathological examinations from January 2009 to January 2013, retrospectively. We evaluated clinical and dermographical characteristics of all patients such as age, sex, existence of oral mucosal lesions, association of pruritus, duration of disease, presence of triggering factors, features of skin lesions, results of direct immunofluorescence (DIF investigation, drug intake, recurrence and mortality rates and causes of detected mortality. Results: Of the 29 patients included in the study %48,3 were female, %51,7 were male. The average age was 70,17 and the mean duration of disease was 15.41 months. In five cases oral mucosal involvement and in 24 cases pruritus was detected. Drugs in three cases and malignity in one case were assessed as triggering factors. The results of DIF investigation revealed the linear IgG and C3 deposition at the dermoepidermal junction in 12 cases, C3 deposition in 10 cases. The initial treatment was topical corticosteroid in 11, tetracycline and nicotinamide combination in six, systemic corticosteroid in 10 individuals. After initial therapy recurrence appeared in 7 patients in one year. Mortality during hospitalization was observed in 2 cases. Conclusions: We presented the clinical and epidemiological features of patients diagnosed as BP in our clinics in the last three years. Because of having different ethnical originated, heterogeneous population of our city, we believe that our investigation will contribute to having opinion about epidemiology of BP in our country. Nevertheless, prospective, long term and multicentre studies based on

  4. Evaluation of the shape symmetry of bilateral normal corneas in a Chinese population.

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    Fangjun Bao

    Full Text Available PURPOSE: To investigate the bilateral symmetry of the global corneal topography in normal corneas with a wide range of curvature, astigmatism and thickness values. DESIGN: Cross-Sectional Study. METHODS: Topography images were recorded for the anterior and posterior surfaces of 342 participants using a Pentacam. Elevation data were fitted to a general quadratic model that considered both translational and rotational displacements. Comparisons between fellow corneas of estimates of corneal shape parameters (elevation, radius in two main directions, Rx and Ry, and corresponding shape factors, Qx and Qy and corneal position parameters (translational displacements: x0, y0 and z0, and rotational displacements: α, β and γ were statistically analyzed. RESULTS: The general quadratic model provided average RMS of fit errors with the topography data of 1.7±0.6 µm and 5.7±1.3 µm in anterior and posterior corneal surfaces. The comparisons showed highly significant bilateral correlations with the differences between fellow corneas in Rx, Ry, Qx and Qy of anterior and posterior surfaces remaining insignificantly different from zero. Bilateral differences in elevation measurements at randomly-selected points in both corneal central and peripheral areas indicated strong mirror symmetry between fellow corneas. The mean geometric center (x0, y0, z0 of both right and left corneas was located on the temporal side and inferior-temporal side of the apex in anterior and posterior topography map, respectively. Rotational displacement angle α along X axis had similar distributions in bilateral corneas, while rotation angle β along Y axis showed both eyes tilting towards the nasal side. Further, rotation angle γ along Z axis, which is related to corneal astigmatism, showed clear mirror symmetry. CONCLUSIONS: Analysis of corneal topography demonstrated strong and statistically-significant mirror symmetry between bilateral corneas. This characteristic could help

  5. Effect of epithelial debridement on human cornea proteoglycans

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    Soriano E.S.

    2001-01-01

    Full Text Available Corneal transparency is attributed to the regular spacing and diameter of collagen fibrils, and proteoglycans may play a role in fibrillogenesis and matrix assembly. Corneal scar tissue is opaque and this opacity is explained by decreased ultrastructural order that may be related to proteoglycan composition. Thus, the objectives of the present study were to characterize the proteoglycans synthesized by human corneal explants and to investigate the effect of mechanical epithelial debridement. Human corneas unsuitable for transplants were immersed in F-12 culture medium and maintained under tissue culture conditions. The proteoglycans synthesized in 24 h were labeled metabolically by the addition of 35S-sulfate to the medium. These compounds were extracted by 4 M GuHCl and identified by a combination of agarose gel electrophoresis, enzymatic degradation with protease and mucopolysaccharidases, and immunoblotting. Decorin was identified as the main dermatan sulfate proteoglycan and keratan sulfate proteoglycans were also prominent components. When the glycosaminoglycan side chains were analyzed, only keratan sulfate and dermatan sulfate were detected (~50% each. Nevertheless, when these compounds were 35S-labeled metabolically, the label in dermatan sulfate was greater than in keratan sulfate, suggesting a lower synthesis rate for keratan sulfate. 35S-Heparan sulfate also appeared. The removal of the epithelial layer caused a decrease in heparan sulfate labeling and induced the synthesis of dermatan sulfate by the stroma. The increased deposit of dermatan sulfate proteoglycans in the stroma suggests a functional relationship between epithelium and stroma that could be related to the corneal opacity that may appear after epithelial cell debridement.

  6. Trasplante de córnea Cornea transplant

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    A. Garralda

    2006-08-01

    Full Text Available La queratoplastia o trasplante de córnea es una de las técnicas quirúrgicas más antiguas de la oftalmología cuyas indicaciones son: 1 tectónicas, para preservar la anatomía e integridad corneal, 2 clínicas, para eliminar el tejido corneal inflamado en casos refractarios al tratamiento médico, 3 ópticas, para mejorar la agudeza visual y 4 cosméticas para mejorar el aspecto del ojo. El perfeccionamiento en la técnica y en el instrumental, así como en el tratamiento postoperatorio y en los medios de conservación del tejido donante han mejorado la supervivencia de los injertos realizados. El Modelo Pamplona de coordinación de trasplantes del Hospital Virgen del Camino (HVC está considerado como original y único en España, y en la logística de este programa se incluye el protocolo de detección y extracción de córneas así como el de queratoplastias.The keratoplasty, or corena transplant, is one of the oldest surgical techniques in opthalmology, whose indication are: 1 tectonic, in order to preserve corneal anatomy and integrity; 2 clinical, in order to eliminate the inflamed corneal tissue in cases refractory to medical treatment; 3 optical, in order to improve visual acuity; and 4 cosmetic, in order to improve the appearance of the eye. Improvements in technique and instruments, as well as in post-operative treatment and the means of preserving donated tissue, have improved survival of the grafts. The Pamplona Model of transplant coordination of the Virgen del Camino Hospital is considered to be original and unique in Spain. The logistics of this program include the protocol for detection and extraction of corneas as well as for keratoplasties.

  7. Evaluation of ELISA testing for BP180 and BP230 as a diagnostic modality for bullous pemphigoid: a clinical experience.

    Science.gov (United States)

    Keller, Jesse J; Kittridge, Ashley L; Debanne, Sara M; Korman, Neil J

    2016-05-01

    Bullous pemphigoid (BP) is a common autoimmune blistering disorder of the elderly. Several diagnostic modalities are available, including clinical impression, histopathology, direct and indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) detection of pathogenic antibodies. In this study, we aim to examine the utility of the newest test, ELISA, in comparison to the constellation of other tests. We describe our clinical experience in which 170 patients diagnosed with bullous pemphigoid had multiple tests performed. BP180 alone showed a sensitivity of 54 % and specificity of 94 %. The positive predictive value (PPV) is 95 % while the negative predictive value (NPV) is 52 %. BP230 alone yielded a sensitivity of 48 % and specificity of 94 %. The PPV is 94 % and the NPV is 49 %. Using both tests in combination yielded a sensitivity of 66 % and specificity of 89 %. The PPV of at least one of two tests returning positive is 92 % while the NPV of dual negative tests is 58 %. Use of ELISAs for suspected cases of BP are an inadequate standalone test, and are only helpful in making the diagnosis should they return positive. However, they would appear to miss about one-third of cases. PMID:26895534

  8. Diabetic cornea wounds produce significantly weaker electric signals that may contribute to impaired healing.

    Science.gov (United States)

    Shen, Yunyun; Pfluger, Trisha; Ferreira, Fernando; Liang, Jiebing; Navedo, Manuel F; Zeng, Qunli; Reid, Brian; Zhao, Min

    2016-01-01

    Wounds naturally produce electric signals which serve as powerful cues that stimulate and guide cell migration during wound healing. In diabetic patients, impaired wound healing is one of the most challenging complications in diabetes management. A fundamental gap in knowledge is whether diabetic wounds have abnormal electric signaling. Here we used a vibrating probe to demonstrate that diabetic corneas produced significantly weaker wound electric signals than the normal cornea. This was confirmed in three independent animal models of diabetes: db/db, streptozotocin-induced and mice fed a high-fat diet. Spatial measurements illustrated that diabetic cornea wound currents at the wound edge but not wound center were significantly weaker than normal. Time lapse measurements revealed that the electric currents at diabetic corneas lost the normal rising and plateau phases. The abnormal electric signals correlated significantly with impaired wound healing. Immunostaining suggested lower expression of chloride channel 2 and cystic fibrosis transmembrane regulator in diabetic corneal epithelium. Acute high glucose exposure significantly (albeit moderately) reduced electrotaxis of human corneal epithelial cells in vitro, but did not affect the electric currents at cornea wounds. These data suggest that weaker wound electric signals and impaired electrotaxis may contribute to the impaired wound healing in diabetes. PMID:27283241

  9. Bilateral cloudy cornea: is the usual suspect congenital hereditary endothelial dystrophy or stromal dystrophy?

    Science.gov (United States)

    Acar, Banu Torun; Bozkurt, Kansu Tahir; Duman, Erkan; Acar, Suphi

    2016-01-01

    We provide the diagnosis, treatment and follow-up period of a patient with cloudy cornea in both eyes from birth. A 4-year-old girl presented with blurring in both eyes. Penetrating keratoplasty (PK) was performed with the preliminary diagnosis of congenital hereditary endothelial dystrophy in June 2012. According to the pathology report for extracted host tissue, the Descemet's membrane (DM) and endothelium were healthy and diagnosis was reported to be congenital hereditary stromal dystrophy. Deep anterior lamellar keratoplasty was performed on the left eye. The DM was transparent at follow-up. Cornea transplantation is the only choice to provide visual rehabilitation in children with congenital cloudy cornea. However, it is known that the prognosis of traditional PK in the paediatric age group is not good. Therefore, when using alternative keratoplasty (deep anterior lamellar keratoplasty, Descemet's stripping automated endothelial keratoplasty) options, pathological examination of the host tissue should be made. PMID:27107055

  10. Air-pulse OCE for assessment of age-related changes in mouse cornea in vivo

    International Nuclear Information System (INIS)

    We demonstrate the use of phase-stabilized swept source optical coherence elastography (PhS-SSOCE) to assess the relaxation rate of deformation created by a focused air-pulse in tissue-mimicking gelatin phantoms of various concentrations and mouse corneas of different ages in vivo. The results show that the relaxation rate can be quantified and is different for gels with varying concentrations of gelatin and mouse corneas of different ages. The results indicate that gel phantoms with higher concentrations of gelatin as well as older mouse corneas have faster relaxation rates indicating stiffer material. This non-contact and non-invasive measurement technique utilizes low surface displacement amplitude (in µm scale) for tissue excitation and, therefore, can be potentially used to study the biomechanical properties of ocular and other sensitive tissues. (letter)

  11. Differentially expressed wound healing-related microRNAs in the human diabetic cornea.

    Directory of Open Access Journals (Sweden)

    Vincent A Funari

    Full Text Available MicroRNAs are powerful gene expression regulators, but their corneal repertoire and potential changes in corneal diseases remain unknown. Our purpose was to identify miRNAs altered in the human diabetic cornea by microarray analysis, and to examine their effects on wound healing in cultured telomerase-immortalized human corneal epithelial cells (HCEC in vitro. Total RNA was extracted from age-matched human autopsy normal (n=6 and diabetic (n=6 central corneas, Flash Tag end-labeled, and hybridized to Affymetrix® GeneChip® miRNA Arrays. Select miRNAs associated with diabetic cornea were validated by quantitative RT-PCR (Q-PCR and by in situ hybridization (ISH in independent samples. HCEC were transfected with human pre-miR™miRNA precursors (h-miR or their inhibitors (antagomirs using Lipofectamine 2000. Confluent transfected cultures were scratch-wounded with P200 pipette tip. Wound closure was monitored by digital photography. Expression of signaling proteins was detected by immunostaining and Western blot. Using microarrays, 29 miRNAs were identified as differentially expressed in diabetic samples. Two miRNA candidates showing the highest fold increased in expression in the diabetic cornea were confirmed by Q-PCR and further characterized. HCEC transfection with h-miR-146a or h-miR-424 significantly retarded wound closure, but their respective antagomirs significantly enhanced wound healing vs. controls. Cells treated with h-miR-146a or h-miR-424 had decreased p-p38 and p-EGFR staining, but these increased over control levels close to the wound edge upon antagomir treatment. In conclusion, several miRNAs with increased expression in human diabetic central corneas were found. Two such miRNAs inhibited cultured corneal epithelial cell wound healing. Dysregulation of miRNA expression in human diabetic cornea may be an important mediator of abnormal wound healing.

  12. Model of Mass and Heat Transfer during Vacuum Freeze-Drying for Cornea

    OpenAIRE

    Zou Huifen; Ye Sheng; Wang Dexi; Li Huixing; Cao Xiaozhen; Yan Lijun

    2012-01-01

    Cornea is the important apparatus of organism, which has complex cell structure. Heat and mass transfer and thermal parameters during vacuum freeze-drying of keeping corneal activity are studied. The freeze-drying cornea experiments were operated in the homemade vacuum freeze dryer. Pressure of the freeze-drying box was about 50 Pa and temperature was about −10°C by controlled, and operating like this could guarantee survival ratio of the corneal endothelium over the grafting normal. Theory a...

  13. Biochemistry of pro-oxidant and anti-oxidant enzymes in the rabbit cornea irradiated with UVB rays

    Czech Academy of Sciences Publication Activity Database

    Štípek, S.; Čejková, Jitka; Ardan, Taras; Crkovská, J.; Bhuiyan, F.; Andonová, Žaneta; Midelfart, A.

    Prague : organising committee, 2002. s. 34. [International Symposium on Cornea and Contact Lenses .. 07.12.2002-10.12.2002, Prague] R&D Projects: GA ČR GA304/96/0908; GA ČR GA304/00/1635 Institutional research plan: CEZ:MSM 111100001 Keywords : cornea Subject RIV: FF - HEENT, Dentistry

  14. Descemet stripping automated endothelial keratoplasty (DSAEK with thin grafts in patients suffered bullous keratophaty with low preoperative visual acuity

    Directory of Open Access Journals (Sweden)

    S. V. Trufanov

    2014-07-01

    Full Text Available Purpose: To evaluate the results of DSAEK with thin grafts in patients suffered bullous keratophaty with low preoperative visual acuity.Methods: DSAEK with thin grafts the thickness of which was 150‑70 μm was fulfilled in 47 patients (47 eyes suffered bullous keratophaty without visible leukomas in the corneal stroma. Visual acuity prior to the operation with a maximum spectacle correction accounted for an average of 0.05±0.04. Tear film osmolarity of 20 patients (20 eyes who participated in the research was measured. Results: In follow-up period graft kept transparency in 39 patients. Visual acuity in 3 months after the operation, on average, without correction was 0.38±0.16, with a maximum of spectacle correction is 0.51± 0.18. The spherical component varied in the range from 0 to 3.75 D, with an average of 1.63 per±1.1 D. Corneal astigmatism was from 0.5 to 4.0 D, an average of 1.8±0.98 D. At preoperative osmolarity indicators were within the normal reference for both operated and non-operated eyes — 292.3±10.4 и 279.3±3.51. In a first postoperative week osmolarity was not detected while on a non-operated eye it was 278.4±1.4. After 1, 3 and 6 months osmolarity indicators on both eyes were within normal reference. Spherical component ranged from 0 to 3.75 D, averaging 1.1±1.63 D. Corneal astigmatism ranged from 0.5 to 4.0 (D, with an average of 1.8±0.98 D 1.63 per±1.1 D.Conclusion: DSAEK with thin grafts is an effective modern methods of surgical treatment of bullous keratophaty. For old patients with severe ocular pathology — concomitant eye diseases, repeated surgery of the eye, the developed stage of the keratophaty — we have not noted the apparent correlation between the thickness of the transplant, visual acuity and the time of recovery of visual functions after keratoplasty. Osmolarity in an early postoperative period is a non-informative method of diagnostics. Restoration of osmolarity level to preoperative

  15. Descemet stripping automated endothelial keratoplasty (DSAEK with thin grafts in patients suffered bullous keratophaty with low preoperative visual acuity

    Directory of Open Access Journals (Sweden)

    S. V. Trufanov

    2013-01-01

    Full Text Available Purpose: To evaluate the results of DSAEK with thin grafts in patients suffered bullous keratophaty with low preoperative visual acuity.Methods: DSAEK with thin grafts the thickness of which was 150‑70 μm was fulfilled in 47 patients (47 eyes suffered bullous keratophaty without visible leukomas in the corneal stroma. Visual acuity prior to the operation with a maximum spectacle correction accounted for an average of 0.05±0.04. Tear film osmolarity of 20 patients (20 eyes who participated in the research was measured. Results: In follow-up period graft kept transparency in 39 patients. Visual acuity in 3 months after the operation, on average, without correction was 0.38±0.16, with a maximum of spectacle correction is 0.51± 0.18. The spherical component varied in the range from 0 to 3.75 D, with an average of 1.63 per±1.1 D. Corneal astigmatism was from 0.5 to 4.0 D, an average of 1.8±0.98 D. At preoperative osmolarity indicators were within the normal reference for both operated and non-operated eyes — 292.3±10.4 и 279.3±3.51. In a first postoperative week osmolarity was not detected while on a non-operated eye it was 278.4±1.4. After 1, 3 and 6 months osmolarity indicators on both eyes were within normal reference. Spherical component ranged from 0 to 3.75 D, averaging 1.1±1.63 D. Corneal astigmatism ranged from 0.5 to 4.0 (D, with an average of 1.8±0.98 D 1.63 per±1.1 D.Conclusion: DSAEK with thin grafts is an effective modern methods of surgical treatment of bullous keratophaty. For old patients with severe ocular pathology — concomitant eye diseases, repeated surgery of the eye, the developed stage of the keratophaty — we have not noted the apparent correlation between the thickness of the transplant, visual acuity and the time of recovery of visual functions after keratoplasty. Osmolarity in an early postoperative period is a non-informative method of diagnostics. Restoration of osmolarity level to preoperative

  16. Bullous pemphigoid and neurodegenerative diseases: a study in a setting of a Central European university dermatology department.

    Science.gov (United States)

    Pietkiewicz, Paweł; Gornowicz-Porowska, Justyna; Bowszyc-Dmochowska, Monika; Bartkiewicz, Paweł; Dmochowski, Marian

    2016-08-01

    Bullous pemphigoid (BP) is an autoimmune blistering dermatosis of the elderly mediated by IgG and IgE antibodies to skin hemidesmosomal proteins, BP180 and/or BP230, that occur physiologically also in neuronal tissue. It was reported that BP is associated with neurodegenerative diseases (ND). We performed a retrospective study in a setting of a Central European university dermatology department on prevalence of ND in 94 BP patients. 26 out of 94 BP patients had at least one ND. ND included: Parkinson's disease, dementia, stroke, hear loss, tinnitus, blindness, vertigo, neurosyphilis, systemic sclerosis, and epilepsy. Since population aging is conceivably responsible for the rising number of BP cases as a result of immunosenescence-related phenomena, the plausible BP-specific immunopathogenetic relationship between BP and ND deserves to be further experimentally explored. PMID:26420424

  17. Crosslinked collagen-gelatin-hyaluronic acid biomimetic film for cornea tissue engineering applications

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yang; Ren, Li, E-mail: psliren@scut.edu.cn; Wang, Yingjun, E-mail: imwangyj@163.com

    2013-01-01

    Cornea disease may lead to blindness and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents the crosslinked collagen (Col)-gelatin (Gel)-hyaluronic acid (HA) films developed by making use of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as the crosslinker. The test results on the physical and biological properties indicate that the CGH631 film (the mass ratio of Col:Gel:HA = 6:3:1) has appropriate optical performance, hydrophilicity and mechanical properties. The diffusion properties of the CGH631 film to NaCl and tryptophan are also satisfactory and the measured data are 2.43 Multiplication-Sign 10{sup -6} cm{sup 2}/s and 7.97 Multiplication-Sign 10{sup -7} cm{sup 2}/s, respectively. In addition, cell viability studies demonstrate that the CGH631 film has good biocompatibility, on which human corneal epithelial cells attached and proliferated well. This biocompatible film may have potential use in cornea tissue engineering. - Highlights: Black-Right-Pointing-Pointer Crosslinked collagen-gelatin-hyaluronic acid films were fabricated in this study. Black-Right-Pointing-Pointer The film had appropriate physical properties. Black-Right-Pointing-Pointer Diffusion coefficient of the film was comparable with the human cornea. Black-Right-Pointing-Pointer HCEC viability studies confirmed the biocompatibility of the film.

  18. Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

    International Nuclear Information System (INIS)

    Cornea disease may lead to blindness and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents the crosslinked collagen (Col)–gelatin (Gel)–hyaluronic acid (HA) films developed by making use of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as the crosslinker. The test results on the physical and biological properties indicate that the CGH631 film (the mass ratio of Col:Gel:HA = 6:3:1) has appropriate optical performance, hydrophilicity and mechanical properties. The diffusion properties of the CGH631 film to NaCl and tryptophan are also satisfactory and the measured data are 2.43 × 10−6 cm2/s and 7.97 × 10−7 cm2/s, respectively. In addition, cell viability studies demonstrate that the CGH631 film has good biocompatibility, on which human corneal epithelial cells attached and proliferated well. This biocompatible film may have potential use in cornea tissue engineering. - Highlights: ► Crosslinked collagen–gelatin–hyaluronic acid films were fabricated in this study. ► The film had appropriate physical properties. ► Diffusion coefficient of the film was comparable with the human cornea. ► HCEC viability studies confirmed the biocompatibility of the film.

  19. Pathways and Mechanisms Underlying the Photophysics and Photochemistry of Riboflavin induced cornea crosslinking

    DEFF Research Database (Denmark)

    Breitenbach, Thomas; Ogilby, Peter Remsen

    In this talk, we will describe general pathways involved in the photophysics of a photosensitized process, which can lead to crosslinking due to light excitation of Riboflavin in the cornea. Furthermore, we will elucidate different aspects of reactions that can produce crosslinks, with respect to...

  20. Eye evolution: lens and cornea as an upgrade of animal visual system

    Czech Academy of Sciences Publication Activity Database

    Jonášová, Kristýna; Kozmik, Zbyněk

    2008-01-01

    Roč. 19, č. 2 (2008), s. 71-81. ISSN 1084-9521 R&D Projects: GA AV ČR IAA500520604; GA MŠk(CZ) 1M0520 Institutional research plan: CEZ:AV0Z50520514 Keywords : eye * lens * cornea Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.528, year: 2008

  1. A structural model for the in vivo human cornea including collagen-swelling interaction.

    Science.gov (United States)

    Cheng, Xi; Petsche, Steven J; Pinsky, Peter M

    2015-08-01

    A structural model of the in vivo cornea, which accounts for tissue swelling behaviour, for the three-dimensional organization of stromal fibres and for collagen-swelling interaction, is proposed. Modelled as a binary electrolyte gel in thermodynamic equilibrium, the stromal electrostatic free energy is based on the mean-field approximation. To account for active endothelial ionic transport in the in vivo cornea, which modulates osmotic pressure and hydration, stromal mobile ions are shown to satisfy a modified Boltzmann distribution. The elasticity of the stromal collagen network is modelled based on three-dimensional collagen orientation probability distributions for every point in the stroma obtained by synthesizing X-ray diffraction data for azimuthal angle distributions and second harmonic-generated image processing for inclination angle distributions. The model is implemented in a finite-element framework and employed to predict free and confined swelling of stroma in an ionic bath. For the in vivo cornea, the model is used to predict corneal swelling due to increasing intraocular pressure (IOP) and is adapted to model swelling in Fuchs' corneal dystrophy. The biomechanical response of the in vivo cornea to a typical LASIK surgery for myopia is analysed, including tissue fluid pressure and swelling responses. The model provides a new interpretation of the corneal active hydration control (pump-leak) mechanism based on osmotic pressure modulation. The results also illustrate the structural necessity of fibre inclination in stabilizing the corneal refractive surface with respect to changes in tissue hydration and IOP. PMID:26156299

  2. Spectroscopic measurements and terahertz imaging of the cornea using a rapid scanning terahertz time domain spectrometer

    Science.gov (United States)

    Wen-Quan, Liu; Yuan-Fu, Lu; Guo-Hua, Jiao; Xian-Feng, Chen; Zhi-Sheng, Zhou; Rong-Bin, She; Jin-Ying, Li; Si-Hai, Chen; Yu-Ming, Dong; Jian-Cheng, Lv

    2016-06-01

    Spectroscopic measurements and terahertz imaging of the cornea are carried out by using a rapid scanning terahertz time domain spectroscopy (THz-TDS) system. A voice coil motor stage based optical delay line (VCM-ODL) is developed to provide a rather simple and robust structure with both the high scanning speed and the large delay length. The developed system is used for THz spectroscopic measurements and imaging of the corneal tissue with different amounts of water content, and the measurement results show the consistence with the reported results, in which the measurement time using VCM-ODL is a factor of 360 shorter than the traditional motorized optical delay line (MDL). With reducing the water content a monotonic decrease of the complex permittivity of the cornea is observed. The two-term Debye relaxation model is employed to explain our experimental results, revealing that the fast relaxation time of a dehydrated cornea is much larger than that of a hydrated cornea and its dielectric behavior can be affected by the presence of the biological macromolecules. These results demonstrate that our THz spectrometer may be a promising candidate for tissue hydration sensing and practical application of THz technology. Project supported by the National Natural Science Foundation of China (Grant No. 61205101), the Shenzhen Municipal Research Foundation, China (Grant Nos. GJHZ201404171134305 and JCYJ20140417113130693), and the Marie Curie Actions-International Research Staff Exchange Scheme (IRSES) (Grant No. FP7 PIRSES-2013-612267).

  3. Pretreatment methods to improve nerve immunostaining in corneas from long-term fixed embryonic quail eyes

    Science.gov (United States)

    Barrett, J. E.; Wells, D. C.; Conrad, G. W.

    1999-01-01

    Pretreatment methods were used to improve neurofilament immunostaining in corneas from embryonic day 16 Japanese quail corneas that had been stored in fixative solution for several months. A sequential combination of the following three pretreatments: brief microwave heating in saline, followed by extraction with sodium dodecyl sulfate (SDS) at 37 degrees C, followed by digestion with hyaluronidase at 37 degrees C, produced significantly increased antibody staining of corneal neurofilament proteins, compared with embryonic corneas subjected to no prior pretreatments or to single or two-step protocols. After applying the sequence of all three pretreatments, darkest nerve staining and increased numbers of fine branches were observed, together with lower background staining. Thus, the result of applying the three-step pretreatment sequence is better than that of applying any of its component single pretreatments or even combinations of any two of them. These findings therefore suggest that each of these three pretreatments causes a unique effect, beneficial to immunostaining of neurofilament proteins, and that their individual effects are independent and additive. In addition to embryonic corneas, the three-step procedure also may be useful for immunostaining of nerves in other very delicate, highly-hydrated tissues containing an abundance of extracellular matrix.

  4. Simultaneous microstructural and mechanical characterization of human corneas at increasing pressure.

    Science.gov (United States)

    Benoit, Aurélie; Latour, Gaël; Marie-Claire, Schanne-Klein; Allain, Jean-Marc

    2016-07-01

    The cornea, through its shape, is the main contributor to the eye׳s focusing power. Pathological alterations of the cornea strongly affect the eye power. To improve treatments, complex biomechanical models have been developed based on the architecture and mechanical properties of the collagen network in the stroma, the main layer of the cornea. However, direct investigations of the structure of the stroma, as well as its link to the mechanical response, remained limited. We propose here an original set up, associating nonlinear optical imaging and mechanical testing. By using polarization resolved Second Harmonic signals, we simultaneously quantified micrometer (orientation of the collagen lamellae) and nanometer (local disorder within lamellae) scale corneal organization. We showed that the organization of the lamellae changes along the stroma thickness. Then, we measured simultaneously the deformation on the epithelial side of the cornea and the reorientation of the collagen lamellae for increasing intraocular pressure levels, from physiological ones to pathological ones. We showed that the observed deformation is not correlated to initial orientation, but to the reorganization of the lamellae in the stroma. Our results, by providing a direct multi-scale observation, will be useful for the development of more accurate biomechanical models. PMID:26773650

  5. Shape of the anterior cornea : Comparison of height data from 4 corneal topographers

    NARCIS (Netherlands)

    de Jong, Tim; Sheehan, Matthew T.; Dubbelman, Michiel; Koopmans, Steven A.; Jansonius, Nomdo M.

    2013-01-01

    PURPOSE: To compare the ability of clinical corneal topographers to describe the shape of the anterior cornea for optical modeling. SETTING: University Medical Center Groningen, Groningen, Netherlands. DESIGN: Cross-sectional study. METHODS: The anterior corneal shape of healthy subjects was assesse

  6. Oxygen transport through soft contact lens and cornea: Lens characterization and metabolic modeling

    Science.gov (United States)

    Chhabra, Mahendra

    The human cornea requires oxygen to sustain metabolic processes critical for its normal functioning. Any restriction to corneal oxygen supply from the external environment (e.g., by wearing a low oxygen-permeability contact lens) can lead to hypoxia, which may cause corneal edema (swelling), limbal hyperemia, neovascularization, and corneal acidosis. The need for adequate oxygen to the cornea is a major driving force for research and development of hypertransmissible soft contact lenses (SCLs). Currently, there is no standard technique for measuring oxygen permeability (Dk) of hypertransmissible silicone-hydrogel SCLs. In this work, an electrochemistry-based polarographic apparatus was designed, built, and operated to measure oxygen permeability in hypertransmissible SCLs. Unlike conventional methods where a range of lens thickness is needed for determining oxygen permeabilities of SCLs, this apparatus requires only a single lens thickness. The single-lens permeameter provides a reliable, efficient, and economic tool for measuring oxygen permeabilities of commercial hypertransmissible SCLs. The single-lens permeameter measures not only the product Dk, but, following modification, it measures separately diffusivity, D, and solubility, k, of oxygen in hypertransmissible SCLs. These properties are critical for designing better lens materials that ensure sufficient oxygen supply to the cornea. Metabolism of oxygen in the cornea is influenced by contact-lens-induced hypoxia, diseases such as diabetes, surgery, and drug treatment, Thus, estimation of the in-vivo corneal oxygen consumption rate is essential for gauging adequate oxygen supply to the cornea. Therefore, we have developed an unsteady-state reactive-diffusion model for the cornea-contact-lens system to determine in-vivo human corneal oxygen-consumption rate. Finally, a metabolic model was developed to determine the relation between contact-lens oxygen transmissibility (Dk/L) and corneal oxygen deficiency. A

  7. Reparative regeneration of cornea at nanostructured biopolymer of hyaluronic acid application

    Directory of Open Access Journals (Sweden)

    V.N. Kanyukov

    2014-04-01

    Full Text Available ABSTRACT Purpose. Estimation of corneal reparative regeneration processes course at application of bioplastic material – nanostructured biopolymer of hyaluronic acid on the model of chemical (alkaline and acid cornea burn. Material and methods. Experimental modeling of cornea chemical burn was carried out on 36 rabbits (72 eyes. The study had two series of cornea burn: alkaline (18 rabbits – 36 eyes and acid (18 rabbits – 36 eyes corneal burns. In each of the series there was identified: an experimental group, which used the applique of bioplastic material «hyamatrix» according to the method of prof. V.N. Kanyukov and control one with Solcoseryl instillations. The clinical study included an examination of the eye anterior segment using the focus and side lighting and photographic recording. At the of period of 3, 7, 14, 30 and 90 days the animals were removed from the experiment for the light-optical, immunocytochemistry and electron microscopy studies. Results. At conducting «hyamatrix» application conjunctiva edema and injection, corneal edema were reversed faster that reduced neovascularization risk in the outcome of corneal alkaline burn. Morphological studies at different periods of the experiment made it possible to determine the sequence of processes from the moment of cornea chemical burns application until the completion of its restoration, which were different in the experimental and control groups. Symptoms of toxic effect of the damaged cells decay products were determined morphologically. Endothelial cells remained intact. The use of «hyamatrix» application allowed reducing the recovery time with the improvement of cornea reparative processes. Conclusion. 1. Application of bioplastic material to the cornea in the early stages of cornea injuries treatment (alkaline and acid burn reduces the severity of edema and hyperemia, and as a result reduces the exudative phase of inflammation. 2. As a result of clinical and

  8. Low-dose radiation effects on the evolution of chronic dystrophical processes in cornea and clouding of crystalline lens

    International Nuclear Information System (INIS)

    Low-dose radiation effects on the course of chronic dystrophical processes in cornea and the dynamics of crystalline lens clouding of involution age genesis are investigated in the patients participated in Chernobyl accident response. Examples of the concrete pathological cases are considered. It was stated that the above dose effects led to exacerbation of the chronic slack dystrophical processes in cornea and intensification of the development of cornea primary dystrophy. In a number of cases the intensification of development of crystalline lens clouding takes place resulted in the cataract for 2-3 years

  9. Transfer of mesenchymal stem cells and cyclosporine A on alkali-injured rabbit cornea using nanofiber scaffolds strongly reduces corneal neovascularization and scar formation.

    Science.gov (United States)

    Cejka, Cestmir; Cejkova, Jitka; Trosan, Peter; Zajicova, Alena; Sykova, Eva; Holan, Vladimir

    2016-09-01

    The aim of this study was to examine whether nanofiber scaffolds seeded with rabbit bone marrow mesenchymal stem cells (MSCs nanofibers) transferred onto the damaged corneal surface and covered with cyclosporine A (CsA)-loaded nanofiber scaffolds (CsA nanofibers) enable healing of the rabbit cornea injured with 1N NaOH. The healing of damaged corneas was examined morphologically, immunohistochemically and biochemically on day 24 after the injury. Compared to untreated injured corneas, where corneal ulceration or large corneal thinning or even perforation were developed, injured corneas treated with drug free nanofibers healed without profound disturbances in a majority of cases, although with fibrosis and scar formation. In injured corneas treated with CsA nanofibers or MSCs nanofibers, the development of scar formation was reduced. Best healing results were obtained with a combination of MSCs and CsA nanofibers (MSCs-CsA nanofibers). Corneas healed with highly restored transparency. Neovascularization highly expressed in untreated injured corneas and reduced in corneas treated with CsA nanofibers or MSCs nanofibers, was suppressed in corneas treated with MSCs-CsA nanofibers. The levels of matrix metalloproteinase 9, inducible nitric oxide synthase, interleukin 6, α-smooth muscle actin, tumor growth factor β and vascular endothelial growth factor were significantly decreased in these corneas as compared to untreated corneas, where the levels of the above mentioned markers were high. In conclusion, MSCs-CsA nanofibers were effective in the treatment of severe alkali-induced corneal injury. PMID:26797822

  10. IgG BULLOUS PEMPHIGOID WITH ANTIBODIES TO IgD, DERMAL BLOOD VESSELS, ECCRINE GLANDS AND THE ENDOMYSIUM OF MONKEY ESOPHAGUS

    OpenAIRE

    Abreu Velez Ana Maria; Girard Julia G.; Howard Michael S.

    2011-01-01

    Context: Bullous pemphigoid is mediated by autoantibodies primarily targeting two structural proteins of basement membrane hemidesmosomes, BP180 (BPAG2; collagen XVII) and BP230 (BPAG1). Case Report: A 70-year-old Caucasian male patient was evaluated for a seven day history of multiple itching, erythematous blisters on his extremities. Biopsies for hematoxylin and eosin examination, direct immunofluorescence and indirect immunofluorescence (including salt split skin analysis) were performed....

  11. Non-O1 Vibrio cholerae inguinal skin and soft tissue infection with bullous skin lesions in a patient with a penis squamous cell carcinoma

    OpenAIRE

    García-Tutor Emilio; del Pozo Jose L; Yuste Jose R; Portillo María E; Aguinaga Aitziber; Pérez-Gracia Jose L; Leiva José

    2009-01-01

    Abstract Vibrio spp. is a pathogen rarely isolated in cancer patients, and in most cases it is associated with haematological diseases. Cutaneous manifestations of this organism are even rarer. We report a case of Non-O1 Vibrio cholerae inguinal skin and soft tissue infection presenting bullous skin lesions in a young type II diabetic patient with a penis squamous cell carcinoma having a seawater exposure history.

  12. Non-O1 Vibrio cholerae inguinal skin and soft tissue infection with bullous skin lesions in a patient with a penis squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    García-Tutor Emilio

    2009-05-01

    Full Text Available Abstract Vibrio spp. is a pathogen rarely isolated in cancer patients, and in most cases it is associated with haematological diseases. Cutaneous manifestations of this organism are even rarer. We report a case of Non-O1 Vibrio cholerae inguinal skin and soft tissue infection presenting bullous skin lesions in a young type II diabetic patient with a penis squamous cell carcinoma having a seawater exposure history.

  13. Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

    OpenAIRE

    van Beek Nina; Rentzsch Kristin; Probst Christian; Komorowski Lars; Kasperkiewicz Michael; Fechner Kai; Bloecker Inga M; Zillikens Detlef; Stöcker Winfried; Schmidt Enno

    2012-01-01

    Abstract Background Various antigen-specific immunoassays are available for the serological diagnosis of autoimmune bullous diseases. However, a spectrum of different tissue-based and monovalent antigen-specific assays is required to establish the diagnosis. BIOCHIP mosaics consisting of different antigen substrates allow polyvalent immunofluorescence (IF) tests and provide antibody profiles in a single incubation. Methods Slides for indirect IF were prepared, containing BIOCHIPS with the fol...

  14. Lúpus eritematoso sistêmico bolhoso na infância: relato de caso Bullous systemic lupus erythematosus in the childhood: case report

    Directory of Open Access Journals (Sweden)

    Mariana Discacciati Chiminazzo

    2007-06-01

    Full Text Available É apresentado caso de lúpus eritematoso bolhoso na infância. Doente do sexo feminino, com nove anos, apresentava erupção vesicobolhosa no tronco, região cervical, genital e membros. O exame anatomopatológico mostrou bolha subepidérmica com neutrófilos, a imunofluorescência direta revelou depósito linear de IgA, IgM, IgG e C3 na zona da membrana basal, e a indireta foi negativa. Os anticorpos antinucleares e o anti-Sm estavam positivos. Houve regressão do quadro com dapsona e prednisona. Trata-se de caso raro de lúpus eritematoso sistêmico (LES na infância que se iniciou com bolhas disseminadas. O LES bolhoso deve ser incluído no diagnóstico diferencial das erupções bolhosas na infância.We report a case of bullous systemic lupus erythematosus in a 9-year-old female, with a vesiculobullous eruption on the trunk, neck, genitals and limbs. A skin biopsy specimen showed subepidermal blister with neutrophils. Direct immunofluorescence revealed deposition of IgA, IgG, IgM and C3 at the epithelial basement membrane zone and indirect immunofluorescence was negative. Antinuclear antibodies and anti-Sm were positive. Resolution of the blisters occurred following treatment with dapsone and prednisone. This is a rare case of systemic lupus erythematous (SLE in the childhood, whose initial manifestation was a bullous eruption. Bullous SLE should be considered in the differential diagnosis of children presenting with generalized bullous eruption.

  15. Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy.

    Science.gov (United States)

    Cejkova, Jitka; Cejka, Cestmir; Trosan, Peter; Zajicova, Alena; Sykova, Eva; Holan, Vladimir

    2016-06-01

    In this study we tried to develop a new approach to suppress inflammation and neovascularization in the alkali-injured rabbit cornea. For this reason Cyclosporine A (CsA)-loaded electrospun nanofibers were transferred onto the ocular surface injured with alkali (0.25 N NaOH). Damaged corneas were divided into the following groups: untreated, treated with CsA eye drops, treated with nanofibers drug-free and treated with CsA-loaded nanofibers. Healthy rabbit corneas served as controls. Drug-free nanofibers and CsA-loaded nanofibers were transferred onto the damaged corneal surface immediately after the injury and sutured to conjunctiva. On day five after the injury the nanofibers were removed. The animals from all groups were sacrificed on day twelve after the injury. The extent of the inflammatory reaction and corneal healing were examined macroscopically, immunohistochemically and biochemically. The central corneal thickness was measured using an ultrasonic pachymeter. When compared with untreated injured corneas, injured corneas treated with drug-free nanofibers or injured corneas treated with CsA eye drops, the number of CD3-positive cells (T lymphocytes) and the production of pro-inflammatory cytokines were strongly reduced in corneas treated with CsA-loaded nanofibers, which was associated with the significantly decreased expression of matrix metalloproteinase 9, inducible nitric oxide synthase, vascular endothelial growth factor and active caspase-3. CsA-loaded nanofibers effectively suppressed corneal inflammation and corneal neovascularization. Central corneal thickness restored to levels before injury only in corneas treated with CsA-loaded nanofibers. Corneal transparency was highly restored in these corneas. It is suggested that the beneficial effect of CsA-loaded nanofibers was associated with the continuous release of CsA from nanofibers and continuous affection of damaged cornea by CsA. The suture of nanofibers to conjunctiva and the closed eyes

  16. Brittle Cornea Syndrome Associated with a Missense Mutation in the Zinc-Finger 469 Gene

    DEFF Research Database (Denmark)

    Christensen, Anne Elisabeth; Knappskog, Per Morten; Midtbø, Marit;

    2010-01-01

    Purpose: To investigate the diverse clinical manifestations, identify the causative mutation and explain the association with red hair in a family with brittle cornea syndrome (BCS). Methods: Eight family members in three generations underwent ophthalmic, dental, and general medical examination...... mapping with SNP markers, DNA sequencing, and MC1R genotyping. Results: At 42 and 48 years of age, respectively, both affected individuals were blind due to retinal detachment and secondary glaucoma. They had extremely thin and bulging corneas, velvety skin, chestnut colored hair, scoliosis, reduced BMD......, dental anomalies, hearing loss and minor cardiac defects. The morphologies of the skin biopsies were normal except that in some areas slightly thinner collagen fibrils were seen in one of the affected individuals. Molecular genetic analysis revealed a novel missense mutation of ZNF469, c.10016G>A that...

  17. Entertainment-education and recruitment of cornea donors: the role of emotion and issue involvement.

    Science.gov (United States)

    Bae, Hyuhn-Suhck

    2008-01-01

    This study examined the role of emotional responses and viewer's level of issue involvement to an entertainment-education show about cornea donation in order to predict intention to register as cornea donors. Results confirmed that sympathy and empathy responses operated as a catalyst for issue involvement, which emerged as an important intermediary in the persuasion process. Issue involvement also was found to be a common causal antecedent of attitude, subjective norm, and perceived behavioral control, the last two of which predict intentions unlike attitude, which does not. The revised path model confirmed that involvement directly influences intention. The findings of this study suggest that adding emotion and involvement in the Theory of Planned Behavior (TPB) enhances the explanatory power of the theory in predicting intentions, which indicates the possibility of combining the Elaboration Likelihood Model (ELM) and the TPB in the prediction of human behaviors. PMID:18307134

  18. Secondary Glaucoma Associated with Encircling Scleral Buckle Migration into the Cornea

    Directory of Open Access Journals (Sweden)

    Şengül Özdek

    2016-01-01

    Full Text Available Transmuscular migration of the encircling band through rectus muscles and straddling of the cornea has only been reported in a few cases previously in the literature. This rare condition has never been associated with glaucoma. In this report, we aimed to describe a unique case with transmuscular migration of encircling buckle as a probable cause of glaucoma. A 17-year-old female presented with transmuscular migration of buckle and high intraocular pressure (IOP. Limbal/corneal migration of the silicone band was thought to be the main reason for the IOP rise; therefore, scleral band removal was performed. One month after removal, the patient was free of glaucoma medications and IOP was within normal limits. The retina remained attached during all postoperative visits. Transmuscular migration of the encircling band through rectus muscles and straddling of the cornea may act as a trigger for glaucoma.

  19. A Method to Prepare a Descemet's Stripping Automated Endothelial Keratoplasty (DSAEK) Graft Using Donor Corneas With Narrow Scleral Rims

    OpenAIRE

    Lin, Tzu-Yu; Hwang, Yih-Shiou; Ma, David Hui-Kang

    2015-01-01

    Abstract Donor corneas with narrow scleral rims are often disqualified for Descemet's Stripping Automated Endothelial Keratoplasty (DSAEK), mainly because of fluid leak and low pressure when they are mounted onto an artificial anterior chamber (AAC). This report describes a novel method to tight-lock a donor cornea with a narrow scleral rim so that microkeratome cutting is possible, allowing a DSAEK procedure to be completed. A 50-year-old male suffering from Epstein–Barr virus (EBV) endothel...

  20. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    OpenAIRE

    Seunghun Lee; Jun Ho Lee; Jin Hyoung Park; Yeoreum Yoon; Wan Kyun Chung; Hungwon Tchah; Myoung Joon Kim; Ki Hean Kim

    2016-01-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. I...

  1. Transient postoperative vascular endothelial growth factor (VEGF)-neutralisation improves graft survival in corneas with partly regressed inflammatory neovascularisation

    OpenAIRE

    Bachmann, B. O.; Lütjen-Drecoll, Elke; F. Bock; Wiegand, S. J.; Hos, D.; Dana, R; Kruse, F. E.; Cursiefen, C.

    2009-01-01

    Background: High-risk keratoplasties are usually performed after an uninflamed and quiescent interval in corneas with partly regressed blood and lymphatic vessels. We analysed whether the inhibition of post-keratoplasty revascularisation in mice with partly regressed corneal vessels (“intermediate-risk”) improves graft survival. Methods: Three interrupted stromal sutures (11-0) in corneas of Balb/c mice (6–8 weeks old) were placed for 6 weeks. Six months after suture removal, penetrating k...

  2. Premalignant melanosis of the conjunctiva and the cornea in xeroderma pigmentosum.

    OpenAIRE

    Paridaens, A D; McCartney, A C; Hungerford, J. L.

    1992-01-01

    Xeroderma pigmentosum is a rare autosomal recessive dermatosis. The neoplastic changes in sunlight-exposed areas of the skin and eyes may be related to the impaired replication of ultraviolet radiation-damaged DNA. A 38-year-old Greek woman is reported with a mild form of xeroderma pigmentosum and primary acquired melanosis with atypia of her right limbal conjunctiva and cornea. The development of this precursor of conjunctival malignant melanoma in a xeroderma pigmentosum patient may support...

  3. Proteoglycan biosynthesis by human corneas from patients with types 1 and 2 macular corneal dystrophy

    International Nuclear Information System (INIS)

    Corneal buttons were obtained from patients with types 1 and 2 macular corneal dystrophy (MCD) and from control patients with Fuchs' dystrophy or keratoconus. Buttons were incubated for 20 h in the presence of [3H]glucosamine or [2-3H]mannose. Radiolabeled proteoglycans and lactosaminoglycan-glycoproteins (L-GPs) were purified using chromatography on Q-Sepharose, Superose 6, and octyl-Sepharose. They were identified using chondroitinase ABC, keratanase or endo-beta-galactosidase digestion, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis or Superose 6 chromatography. This study confirms previous reports that type 1 MCD corneas synthesize a normal dermatan sulfate-proteoglycan (DS-PG) and an abnormal keratan sulfate-proteoglycan (KS-PG). The data indicate that typ 1 MCD corneas synthesize L-GP instead of KS-PG. This L-GP has a core protein of similar hydrophobicity (elution from octyl-Sepharose) and nearly similar mass (42 kDa) as the core protein of the KS-PG. It has identical glycoconjugates as those of the KS-PG except that they lack sulfate. Thus, type 1 MCD fails to synthesize keratan sulfate as a result of a defect in a sulfotransferase specific for sulfating lactosaminoglycans. Further, proteoglycans synthesized by a cornea from a patient with type 2 MCD were studied. This cornea synthesized a normal ratio of KS-PG to DS-PG although net synthesis of proteoglycans was approximately 30% below normal. The KS-PG appeared normal whereas the DS-PG had dermatan sulfate chains that were approximately 40% shorter than normal

  4. Proteoglycan biosynthesis by human corneas from patients with types 1 and 2 macular corneal dystrophy

    Energy Technology Data Exchange (ETDEWEB)

    Midura, R.J.; Hascall, V.C.; MacCallum, D.K.; Meyer, R.F.; Thonar, E.J.; Hassell, J.R.; Smith, C.F.; Klintworth, G.K. (National Institute of Dental Research, Bethesda, MD (USA))

    1990-09-15

    Corneal buttons were obtained from patients with types 1 and 2 macular corneal dystrophy (MCD) and from control patients with Fuchs' dystrophy or keratoconus. Buttons were incubated for 20 h in the presence of (3H)glucosamine or (2-3H)mannose. Radiolabeled proteoglycans and lactosaminoglycan-glycoproteins (L-GPs) were purified using chromatography on Q-Sepharose, Superose 6, and octyl-Sepharose. They were identified using chondroitinase ABC, keratanase or endo-beta-galactosidase digestion, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis or Superose 6 chromatography. This study confirms previous reports that type 1 MCD corneas synthesize a normal dermatan sulfate-proteoglycan (DS-PG) and an abnormal keratan sulfate-proteoglycan (KS-PG). The data indicate that typ 1 MCD corneas synthesize L-GP instead of KS-PG. This L-GP has a core protein of similar hydrophobicity (elution from octyl-Sepharose) and nearly similar mass (42 kDa) as the core protein of the KS-PG. It has identical glycoconjugates as those of the KS-PG except that they lack sulfate. Thus, type 1 MCD fails to synthesize keratan sulfate as a result of a defect in a sulfotransferase specific for sulfating lactosaminoglycans. Further, proteoglycans synthesized by a cornea from a patient with type 2 MCD were studied. This cornea synthesized a normal ratio of KS-PG to DS-PG although net synthesis of proteoglycans was approximately 30% below normal. The KS-PG appeared normal whereas the DS-PG had dermatan sulfate chains that were approximately 40% shorter than normal.

  5. Activation of Master Autophagy Regulator TFEB During Systemic LPS Administration in the Cornea

    OpenAIRE

    Uchida, Kyoko; Unuma, Kana; Funakoshi, Takeshi; Aki, Toshihiko; Uemura, Koichi

    2014-01-01

    The involvement of autophagy in the cornea during the systemic inflammatory response elicited by intravenous administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague-Dawley rats were injected i.v. with 15 mg/kg body weight LPS. RC4 rabbit corneal keratocytes were also used and treated with 100 ng/mL of tumor necrosis factor α (TNFα) and/or cycloheximide (CHX). The nuclear translocation of transcription factor EB (TFEB), the master transcriptional regulator for ...

  6. Hyperglycemia-induced abnormalities in rat and human corneas: the potential of second harmonic generation microscopy.

    OpenAIRE

    Latour, Gaël; Kowalczuk, Laura; Savoldelli, Michèle; Bourges, Jean-Louis; Plamann, Karsten; Behar-Cohen, Francine; Schanne-Klein, Marie-Claire

    2012-01-01

    BACKGROUND: Second Harmonic Generation (SHG) microscopy recently appeared as an efficient optical imaging technique to probe unstained collagen-rich tissues like cornea. Moreover, corneal remodeling occurs in many diseases and precise characterization requires overcoming the limitations of conventional techniques. In this work, we focus on diabetes, which affects hundreds of million people worldwide and most often leads to diabetic retinopathy, with no early diagnostic tool. This study then a...

  7. Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.

    Directory of Open Access Journals (Sweden)

    Hassan Alizadeh

    Full Text Available Free-living amoebae of the Acanthamoeba species are the causative agent of Acanthamoeba keratitis (AK, a sight-threatening corneal infection that causes severe pain and a characteristic ring-shaped corneal infiltrate. Innate immune responses play an important role in resistance against AK. The aim of this study is to determine if Toll-like receptors (TLRs on corneal epithelial cells are activated by Acanthamoeba, leading to initiation of inflammatory responses in the cornea. Human corneal epithelial (HCE cells constitutively expressed TLR1, TLR2, TLR3, TLR4, and TLR9 mRNA, and A. castellanii upregulated TLR4 transcription. Expression of TLR1, TLR2, TLR3, and TLR9 was unchanged when HCE cells were exposed to A. castellanii. IL-8 mRNA expression was upregulated in HCE cells exposed to A. castellanii. A. castellanii and lipopolysaccharide (LPS induced significant IL-8 production by HCE cells as measured by ELISA. The percentage of total cells positive for TLR4 was higher in A. castellanii stimulated HCE cells compared to unstimulated HCE cells. A. castellanii induced upregulation of IL-8 in TLR4 expressing human embryonic kidney (HEK-293 cells, but not TLR3 expressing HEK-293 cells. TLR4 neutralizing antibody inhibited A. castellanii-induced IL-8 by HCE and HEK-293 cells. Clinical strains but not soil strains of Acanthamoeba activated TLR4 expression in Chinese hamster corneas in vivo and in vitro. Clinical isolates but not soil isolates of Acanthamoeba induced significant (P< 0.05 CXCL2 production in Chinese hamster corneas 3 and 7 days after infection, which coincided with increased inflammatory cells in the corneas. Results suggest that pathogenic species of Acanthamoeba activate TLR4 and induce production of CXCL2 in the Chinese hamster model of AK. TLR4 may be a potential target in the development of novel treatment strategies in Acanthamoeba and other microbial infections that activate TLR4 in corneal cells.

  8. A Cornea Substitute Derived from Fish Scale: 6-Month Followup on Rabbit Model

    OpenAIRE

    Fei Yuan; Liyan Wang; Chien-Chen Lin; Cheng-Hung Chou; Lei Li

    2014-01-01

    A fish scale-derived cornea substitute (Biocornea) is proposed as an alternative for human donor corneal tissue. We adopt a regenerative medicine approach to design a primary alternative to the use of fish scale for restoring sight by corneal replacement. Biocornea with corneal multilayer arrangement collagen was implanted to rabbits by pocket implantation. Our study demonstrated the safety and detailed morphologic and physiologic results from the 6 months of followup of rabbit model. In the ...

  9. Cornea and Ocular Surface Disease: Application of Cutting Edge Optometric Research

    OpenAIRE

    Robertson, Danielle M.; Alexander, Larry J.; Bonanno, Joseph A.; Fleiszig, Suzanne M. J.; McNamara, Nancy

    2014-01-01

    Clinician-scientists bridge the gap between basic research and patient care. At the 2012 Annual Meeting, a symposium highlighting the application of cutting edge optometric research within the anterior segment was held to present and discuss some of the recent basic scientific advances that will both shape and guide the development of future clinical care practices. This paper summarizes this work, bringing together four experts, all clinician-scientists in the field of cornea and ocular surf...

  10. Comparison of Scheimpflug imaging parameters between steep and keratoconic corneas of Caucasian eyes

    Science.gov (United States)

    Huseynova, Tukezban; Abdulaliyeva, Farah; Lanza, Michele

    2016-01-01

    Purpose To compare the keratometric and pachymetric parameters of healthy eyes with those affected by steep cornea and keratoconus (KC) using Scheimpflug camera. Setting Briz-L Eye Clinic, Baku, Azerbaijan. Design A cross-sectional study. Methods In this study, 49 KC (Amsler–Krumeich stage 1) eyes and 36 healthy eyes were enrolled. A complete ophthalmic evaluation and a Scheimpflug camera scan were performed in every eye included in the study. Tomographic parameters such as parameters from the front and back cornea, maximum keratometry reading (Kmax), corneal volume (CV), anterior chamber volume (ChV), anterior chamber depth (ACD), anterior chamber angle (AC angle), keratometric power deviation (KPD), maximum front elevation (Max FE), and maximum back elevation (Max BE), as well as pachymetric progression indices (PPI), Ambrosio relational thickness (ART), index of surface variance (ISV), index of vertical asymmetry (IVA), center keratoconus index (CKI), index of height asymmetry (IHA), index of height decentration (IHD), and radius minimum (RM) were collected and statistically compared between the two groups. Results PPI, ART, ISV, IVA, CKI, IHA, IHD, and RM parameter values were significantly different (Pastigmatism, between stage 1 keratoconic and normal Caucasian eyes with steep cornea. All other parameters such as K mean and Q values of the back corneal parameters, Max FE, Max BE, ACD, ChV, and CV showed significant differences between the groups (P<0.05 for all). Conclusion Scheimpflug imaging is able to detect corneal morphological differences between stage 1 KC eyes and healthy eyes with steep cornea, in Caucasians. PMID:27099469

  11. Atmospheric-Pressure Cold Plasma Induces Transcriptional Changes in Ex Vivo Human Corneas.

    Directory of Open Access Journals (Sweden)

    Umberto Rosani

    Full Text Available Atmospheric pressure cold plasma (APCP might be considered a novel tool for tissue disinfection in medicine since the active chemical species produced by low plasma doses, generated by ionizing helium gas in air, induces reactive oxygen species (ROS that kill microorganisms without substantially affecting human cells.In this study, we evaluated morphological and functional changes in human corneas exposed for 2 minutes (min to APCP and tested if the antioxidant n-acetyl l-cysteine (NAC was able to inhibit or prevent damage and cell death.Immunohistochemistry and western blotting analyses of corneal tissues collected at 6 hours (h post-APCP treatment demonstrated no morphological tissue changes, but a transient increased expression of OGG1 glycosylase that returned to control levels in 24 h. Transcriptome sequencing and quantitative real time PCR performed on different corneas revealed in the treated corneas many differentially expressed genes: namely, 256 and 304 genes showing expression changes greater than ± 2 folds in the absence and presence of NAC, respectively. At 6 h post-treatment, the most over-expressed gene categories suggested an active or enhanced cell functioning, with only a minority of genes specifically concerning oxidative DNA damage and repair showing slight over-expression values (<2 folds. Moreover, time-related expression analysis of eight genes up-regulated in the APCP-treated corneas overall demonstrated the return to control expression levels after 24 h.These findings of transient oxidative stress accompanied by wide-range transcriptome adjustments support the further development of APCP as an ocular disinfectant.

  12. Effects of Intracameral Injection of Lidocaine on The Cornea in A Rabbit Model. Scanning Electronmicroscopic Study

    OpenAIRE

    Ramadan, Wafaa S.

    2005-01-01

    Combined topical and intracameral anesthesia proved to be an alternative to peribulbar and retrobulbar anesthesia in cataract surgery especially in phacoemulsification approach.. So, in the present work the effect of intracameral injection of different concentrations of lidocaine on the structure of rabbit's cornea was studied using the freez-fracture technique and the scanning electron microscope . Fourteen baladi rabbits of both sexes weighing 2-3 kg.were used and divided into two groups ac...

  13. Donor cornea preparation in partial big bubble deep anterior lamellar keratoplasty

    OpenAIRE

    Lim L; Lim SW

    2014-01-01

    Li Lim,1 Samuel Wen Yan Lim21Corneal and External Eye Disease Service, Singapore National Eye Centre, 2Yong Loo Lin School of Medicine, National University of Singapore, SingaporeBackground: The purpose of this paper is to describe a technique of donor cornea preparation to ensure good graft-host apposition in incomplete big bubble deep anterior lamellar keratoplasty.Methods: Following a partial-thickness trephination, manual dissection and excision of corneal stroma was performed. Anwar'...

  14. Synthesis of type III collagen by fibroblasts from the embryonic chick cornea

    OpenAIRE

    1980-01-01

    Synthesis of collagen types I, II, III, and IV in cells from the embryonic chick cornea was studied using specific antibodies and immunofluorescence. Synthesis of radioactively labeled collagen types I and III was followed by fluorographic detection of cyanogen bromide peptides on polyacrylamide slab gels and by carboxymethylcellulose chromatography followed by disc gel electrophoresis. Type III collagen had been detected previously by indirect immunofluorescence in the corneal epithelial cel...

  15. Homozygous ALOXE3 Nonsense Variant Identified in a Patient with Non-Bullous Congenital Ichthyosiform Erythroderma Complicated by Superimposed Bullous Majocchi’s Granuloma: The Consequences of Skin Barrier Dysfunction

    Directory of Open Access Journals (Sweden)

    Tao Wang

    2015-09-01

    Full Text Available Non-bullous congenital ichthyosiform erythroderma (NBCIE is a hereditary disorder of keratinization caused by pathogenic variants in genes encoding enzymes important to lipid processing and terminal keratinocyte differentiation. Impaired function of these enzymes can cause pathologic epidermal scaling, significantly reduced skin barrier function. In this study, we have performed a focused, genetic analysis of a probrand affected by NBCIE and extended this to his consanguineous parents. Targeted capture and next-generation sequencing was performed on NBCIE associated genes in the proband and his unaffected consanguineous parents. We identified a homozygous nonsense variant c.814C>T (p.Arg272* in ALOXE3 (NM_001165960.1 in the proband and discovered that his parents are both heterozygous carriers of the variant. The clinical manifestations of the proband’s skin were consistent with NBCIE, and detailed histopathological assessment revealed epidermal bulla formation and Majocchi’s granuloma. Infection with Trichophyton rubrum was confirmed by culture. The patient responded to oral terbinafine antifungal treatment. Decreased skin barrier function, such as that caused by hereditary disorders of keratinization, can increase the risk of severe cutaneous fungal infections and the formation of Majocchi’s granuloma and associated alopecia. Patients with NBCIE should be alerted to the possible predisposition for developing dermatophytoses and warrant close clinical follow-up.

  16. Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea

    Directory of Open Access Journals (Sweden)

    Katsuo Tomoyose

    2015-01-01

    Full Text Available To investigate the effects of loss of transient receptor potential vanilloid receptor 1 (TRPV1 on the development of neovascularization in corneal stroma in mice. Blocking TRPV1 receptor did not affect VEGF-dependent neovascularization in cell culture. Lacking TRPV1 inhibited neovascularization in corneal stroma following cauterization. Immunohistochemistry showed that immunoreactivity for active form of TGFβ1 and VEGF was detected in subepithelial stroma at the site of cauterization in both genotypes of mice, but the immunoreactivity seemed less marked in mice lacking TRPV1. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. TRPV1 gene ablation did not affect invasion of neutrophils and macrophage in a cauterized mouse cornea. Blocking TRPV1 signal does not affect angiogenic effects by HUVECs in vitro. TRPV1 signal is, however, involved in expression of angiogenic growth factors in a cauterized mouse cornea and is required for neovascularization in the corneal stroma in vivo.

  17. Safety of cornea and iris in ocular surgery with 355-nm lasers

    Science.gov (United States)

    Wang, Jenny; Chung, Jae Lim; Schuele, Georg; Vankov, Alexander; Dalal, Roopa; Wiltberger, Michael; Palanker, Daniel

    2015-09-01

    A recent study showed that 355-nm nanosecond lasers cut cornea with similar precision to infrared femtosecond lasers. However, use of ultraviolet wavelength requires precise assessment of ocular safety to determine the range of possible ophthalmic applications. In this study, the 355-nm nanosecond laser was evaluated for corneal and iris damage in rabbit, porcine, and human donor eyes as determined by minimum visible lesion (MVL) observation, live/dead staining of the endothelium, and apoptosis assay. Single-pulse damage to the iris was evaluated on porcine eyes using live/dead staining. In live rabbits, the cumulative median effective dose (ED50) for corneal damage was 231 J/cm2, as seen by lesion observation. Appearance of endothelial damage in live/dead staining or apoptosis occurred at higher radiant exposure of 287 J/cm2. On enucleated rabbit and porcine corneas, ED50 was 87 and 52 J/cm2, respectively, by MVL, and 241 and 160 J/cm2 for endothelial damage. In human eyes, ED50 for MVL was 110 J/cm2 and endothelial damage at 453 J/cm2. Single-pulse iris damage occurred at ED50 of 208 mJ/cm2. These values determine the energy permitted for surgical patterns and can guide development of ophthalmic laser systems. Lower damage threshold in corneas of enucleated eyes versus live rabbits is noted for future safety evaluation.

  18. Reconstruction of auto-tissue-engineered lamellar cornea by dynamic culture for transplantation: a rabbit model.

    Science.gov (United States)

    Wu, Zheng; Zhou, Qiang; Duan, Haoyun; Wang, Xiaoran; Xiao, Jianhui; Duan, Hucheng; Li, Naiyang; Li, Chaoyang; Wan, Pengxia; Liu, Ying; Song, Yiyue; Zhou, Chenjing; Huang, Zheqian; Wang, Zhichong

    2014-01-01

    To construct an auto-tissue-engineered lamellar cornea (ATELC) for transplantation, based on acellular porcine corneal stroma and autologous corneal limbal explants, a dynamic culture process, which composed of a submersion culture, a perfusion culture and a dynamic air-liquid interface culture, was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure and DNA content that were similar to native lamellar cornea (NLC, p>0.05). Compared to NLC, the protein contents of zonula occludens-1, desmocollin-2 and integrin β4 in ATELC-Dynamic reached 93%, 89% and 73%, respectively. The basal cells of ATELC-Dynamic showed a better differentiation phenotype (K3-, P63+, ABCG2+) compared with that of ATELC in static air-lift culture (ATELC-Static, K3+, P63-, ABCG2-). Accordingly, the cell-cloning efficiency of ATELC-Dynamic (9.72±3.5%) was significantly higher than that of ATELC-Static (2.13±1.46%, p0.05). Rabbit lamellar keratoplasty showed that the barrier function of ATELC-Dynamic was intact, and there were no signs of epithelial shedding or neovascularization. Furthermore, the ATELC-Dynamic group had similar optical properties and wound healing processes compared with the NLC group. Thus, the sequential dynamic culture process that was designed according to corneal physiological characteristics could successfully reconstruct an auto-lamellar cornea with favorable morphological characteristics and satisfactory physiological function. PMID:24705327

  19. Maintaining the cornea and the general physiological environment in visual neurophysiology experiments.

    Science.gov (United States)

    Metha, A B; Crane, A M; Rylander, H G; Thomsen, S L; Albrecht, D G

    2001-08-30

    Neurophysiologists have been investigating the responses of neurons in the visual system for the past half-century using monkeys and cats that are anesthetized and paralyzed, with the non-blinking eyelids open for prolonged periods of time. Impermeable plastic contact lenses have been used to prevent dehydration of the corneal epithelium, which would otherwise occur in minutes. Unfortunately, such lenses rapidly introduce a variety of abnormal states that lead to clouding of the cornea, degradation of the retinal image, and premature termination of the experiment. To extend the viability of such preparations, a new protocol for maintenance of corneal health has been developed. The protocol uses rigid gas permeable contact lenses designed to maximize gas transmission, rigorous sterile methods, and a variety of methods for sustaining and monitoring the overall physiology of the animal. The effectiveness of the protocol was evaluated clinically by ophthalmoscopy before, during, and after the experiments, which lasted 8-10 days. Histopathology and quantitative histology were performed on the corneas following the experiment. Our observations showed that this protocol permits continuous contact lens wear without adversely affecting the corneas. Thus, it is possible to collect data 24 h each day, for the entire duration of the experiment. PMID:11513950

  20. A cornea substitute derived from fish scale: 6-month followup on rabbit model.

    Science.gov (United States)

    Yuan, Fei; Wang, Liyan; Lin, Chien-Chen; Chou, Cheng-Hung; Li, Lei

    2014-01-01

    A fish scale-derived cornea substitute (Biocornea) is proposed as an alternative for human donor corneal tissue. We adopt a regenerative medicine approach to design a primary alternative to the use of fish scale for restoring sight by corneal replacement. Biocornea with corneal multilayer arrangement collagen was implanted to rabbits by pocket implantation. Our study demonstrated the safety and detailed morphologic and physiologic results from the 6 months of followup of rabbit model. In the peripheral Biocornea, the collagen fibrils were arranged in reticular fashion. Slit lamp examination showed that haze and an ulcer were not observed in all groups at 3 months postoperatively while all corneas with Biocornea were clear at both 3 months and 6 months postoperatively. The interface of Biocornea and stromal tissue were filled successfully and without observable immune cells at postoperative day 180. Moreover, the Biocornea was not dissolved and degenerated but remained transparent and showed no apparent fragmentation. Our study demonstrated that the Biocornea derived from fish scale as a good substitute had high biocompatibility and support function after a long-term evaluation. This revealed that the new approach of using Biocornea may yield an ideal artificial cornea substitute for long-term inlay placement. PMID:25089206

  1. A Cornea Substitute Derived from Fish Scale: 6-Month Followup on Rabbit Model

    Directory of Open Access Journals (Sweden)

    Fei Yuan

    2014-01-01

    Full Text Available A fish scale-derived cornea substitute (Biocornea is proposed as an alternative for human donor corneal tissue. We adopt a regenerative medicine approach to design a primary alternative to the use of fish scale for restoring sight by corneal replacement. Biocornea with corneal multilayer arrangement collagen was implanted to rabbits by pocket implantation. Our study demonstrated the safety and detailed morphologic and physiologic results from the 6 months of followup of rabbit model. In the peripheral Biocornea, the collagen fibrils were arranged in reticular fashion. Slit lamp examination showed that haze and an ulcer were not observed in all groups at 3 months postoperatively while all corneas with Biocornea were clear at both 3 months and 6 months postoperatively. The interface of Biocornea and stromal tissue were filled successfully and without observable immune cells at postoperative day 180. Moreover, the Biocornea was not dissolved and degenerated but remained transparent and showed no apparent fragmentation. Our study demonstrated that the Biocornea derived from fish scale as a good substitute had high biocompatibility and support function after a long-term evaluation. This revealed that the new approach of using Biocornea may yield an ideal artificial cornea substitute for long-term inlay placement.

  2. Dose-dependent ultrastructural changes in rat cornea after oral methylphenidate administration

    International Nuclear Information System (INIS)

    Objective was to investigate dose-dependent ultrastructural changes in rat cornea after oral methylphenidate (Ritalin) administration. This study was conducted in the Dept. of Anatomy, Gazi University, Faculty of Medicine, Ankara between March and May 2005, with a total of 27 female prepubertal Wistar albino rats, divided into 3 different dose groups (5mg/kg, 10 mg/kg, 20 mg/kg) and their control groups. They were treated orally with methylphenidate and eye tissue was removed to process for electron microscopic studies. We observed that all cells and prominently basal cells of the corneal epithelium show dose-dependent degenerative changes such as apoptotic bodies, chromatin condensation and ondulation in their nuclei and crystolysis of the mitochondrion. In the stroma, the most evident finding was the increase of the collagen fiber. In addition to dose-dependent changes related to apoptotic process, which is chromatin condensation in their nuclei, electron dense material accumulation and percicellular edema in the cytoplasm were also seen. In the endothelial cell lines, disruption of the junctional complexes, vacuolization in the cell cytoplasms and crystolysis of the mitochondrion's with rough endoplasmic reticulum cisternae activity were observed. Ritalin is inducing an evident degeneration, especially in epithelium cells with increasing doses. Ultrastructural cell organelle composition degeneration with stromal fibrosis has negative effect on cornea dehydration. In light of these findings, we believe that the Ritalin treatment dose needed to be kept to a minimum to maintain healthy cornea ultrastructure and related physiology. (author)

  3. Reconstruction of auto-tissue-engineered lamellar cornea by dynamic culture for transplantation: a rabbit model.

    Directory of Open Access Journals (Sweden)

    Zheng Wu

    Full Text Available To construct an auto-tissue-engineered lamellar cornea (ATELC for transplantation, based on acellular porcine corneal stroma and autologous corneal limbal explants, a dynamic culture process, which composed of a submersion culture, a perfusion culture and a dynamic air-liquid interface culture, was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure and DNA content that were similar to native lamellar cornea (NLC, p>0.05. Compared to NLC, the protein contents of zonula occludens-1, desmocollin-2 and integrin β4 in ATELC-Dynamic reached 93%, 89% and 73%, respectively. The basal cells of ATELC-Dynamic showed a better differentiation phenotype (K3-, P63+, ABCG2+ compared with that of ATELC in static air-lift culture (ATELC-Static, K3+, P63-, ABCG2-. Accordingly, the cell-cloning efficiency of ATELC-Dynamic (9.72±3.5% was significantly higher than that of ATELC-Static (2.13±1.46%, p0.05. Rabbit lamellar keratoplasty showed that the barrier function of ATELC-Dynamic was intact, and there were no signs of epithelial shedding or neovascularization. Furthermore, the ATELC-Dynamic group had similar optical properties and wound healing processes compared with the NLC group. Thus, the sequential dynamic culture process that was designed according to corneal physiological characteristics could successfully reconstruct an auto-lamellar cornea with favorable morphological characteristics and satisfactory physiological function.

  4. Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

    Directory of Open Access Journals (Sweden)

    Hieronim Bartel

    2010-08-01

    Full Text Available The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30 in which Ringer liquid was given, and experimental (n=30 in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10th and 19th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea.

  5. Property-based design: optimization and characterization of polyvinyl alcohol (PVA) hydrogel and PVA-matrix composite for artificial cornea.

    Science.gov (United States)

    Jiang, Hong; Zuo, Yi; Zhang, Li; Li, Jidong; Zhang, Aiming; Li, Yubao; Yang, Xiaochao

    2014-03-01

    Each approach for artificial cornea design is toward the same goal: to develop a material that best mimics the important properties of natural cornea. Accordingly, the selection and optimization of corneal substitute should be based on their physicochemical properties. In this study, three types of polyvinyl alcohol (PVA) hydrogels with different polymerization degree (PVA1799, PVA2499 and PVA2699) were prepared by freeze-thawing techniques. After characterization in terms of transparency, water content, water contact angle, mechanical property, root-mean-square roughness and protein adsorption behavior, the optimized PVA2499 hydrogel with similar properties of natural cornea was selected as a matrix material for artificial cornea. Based on this, a biomimetic artificial cornea was fabricated with core-and-skirt structure: a transparent PVA hydrogel core, surrounding by a ringed PVA-matrix composite skirt that composed of graphite, Fe-doped nano hydroxyapatite (n-Fe-HA) and PVA hydrogel. Different ratio of graphite/n-Fe-HA can tune the skirt color from dark brown to light brown, which well simulates the iris color of Oriental eyes. Moreover, morphologic and mechanical examination showed that an integrated core-and-skirt artificial cornea was formed from an interpenetrating polymer network, no phase separation appeared on the interface between the core and the skirt. PMID:24464723

  6. Quantifying the effect of milli-molar glucose concentration on thickness of rabbit cornea with optical coherence tomography

    Science.gov (United States)

    Ghosn, Mohamad G.; Leba, Michael; Vijayananda, Astha; Ansari, Rafat R.; Larin, Kirill V.

    2009-02-01

    The cornea contributes about 65% of the eye's ability to refract light. Thus, any fluctuation in corneal thickness can cause noticeable changes in vision. The presence of glucose molecules induces a driving force for water to leave the collagen fibrils in the cornea due to the concentration gradient created, thus changing its thickness. In this study, the effect of various milli-molar glucose concentrations on corneal thickness was explored using Optical Coherence Tomography. Whole rabbit eyes were placed in a specially designed dish while immersed in saline to ensure proper hydration of the eye. The cornea was imaged for 10 minutes. In 30 minute increments, a higher concentration of glucose was added, bringing the overall glucose concentration to 10, 15, 20, 25, and 30 mM. The thickness of the cornea was measured every 2 minutes. Ultimately, an inverse relationship was observed, indicating that the increase in glucose concentration yielded a decrease in the corneal thickness. From three separate experiments, the cornea experienced 8 +/- 1, 27 +/- 1, 44 +/- 3, 58 +/- 3, and 64 +/- 3 μm decrease in thickness from its starting value while exposed to 10, 15, 20, 25, and 30 mM solutions of glucose, respectively. This relationship provides insight on the physiological changes of the cornea as a result of different glucose concentrations. This could potentially be useful in monitoring blood-glucose levels through the eye.

  7. A CONTROVERSIAL ON THE DIAGNOSIS OF CHRONIC BULLOUS TYPE MUCOCUTANEOUS DISEASE INVOLVING ORAL MUCOSA (A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Isadora Gracia

    2006-04-01

    Full Text Available A case of chronic bullous type mucocutaneous disease involving oral mucosa was reported from a 56 years old man with never healing oral ulcers and wound on the perianal skin for three years. There were also red and black spots on the limb and back skin and a lesion on nail. Painful oral lesion consisted of mucous erosion, desquamative gingivitis, and sloughing area on palate and tongue. The patient is diabetic. The first perianal skin diagnosis was granulomatous candidasis with differential diagnosis pemphigus vegetates and acuminarum condiloma. However the histopathologic examination did not support these diagnosis. After several histopathologic examinations, the latest perianal skin diagnosis was lichen planus with differential diagnosis granulomatous vasculitis, bowenoid papulosis and pyodema gangrenosum. Other skin diagnosis was erythema multiforme. Oral diagnosis was mucous membrane pemphigoid with differential diagnosis lichen planus, Behçet's syndrome and erythema multiforme. Oral histopathologic examinations showed a sub-epithelial blister, which supported mucous membrane pemphigoid. A lip balm, prednisone 5 mg oral rinse and multivitamins were given but oral improvement started after blood sugar level was controlled. Conclusion: It is not yet known whether skin and oral mucous lesions are from the same disease or not.

  8. Pioderma gangrenoso bolhoso e síndrome mielodisplásica Bullous pyoderma gangrenosum and myelodysplastic syndrome

    Directory of Open Access Journals (Sweden)

    Mariana Dias Batista

    2006-10-01

    Full Text Available O pioderma gangrenoso pode apresentar-se como manifestação paraneoplásica. Relata-se um caso de pioderma gangrenoso, da variante bolhosa, acompanhado de bicitopenia, em que foi evidenciado, por meio de mielograma, biópsia de medula óssea e cariótipo, padrão compatível com síndrome mielodisplásica, subtipo citopenia refratária com displasia de multilinhagens. Foi tratado com dapsona, obtendo cicatrização das lesões. O pioderma gangrenoso pode associar-se a doenças sistêmicas, devendo a síndrome mielodisplásica ser considerada nos casos acompanhados de citopenias. Portanto, o pioderma gangrenoso pode ser um marcador cutâneo de doença sistêmica de prognóstico reservado.Pyoderma gangrenosum can present as a cutaneous manifestation of paraneoplastic syndromes. A case of bullous pyoderma gangrenosum associated with bicytopenia is described. During the complementary investigation, myelogram, bone marrow biopsy and karyotype were performed, and showed a pattern consistent with myelodysplastic syndrome. The patient was treated with dapsone with improvement. Pyoderma gangrenosum can be a manifestation of systemic diseases. The possibility of myelodysplastic syndrome should always be considered in patients with pyoderma gangrenosum associated with cytopenia. Pyoderma gangrenosum could indicate poorer prognosis in patients with systemic diseases.

  9. Validation of an endothelial roll preparation for Descemet Membrane Endothelial Keratoplasty by a cornea bank using "no touch" dissection technique.

    Science.gov (United States)

    Marty, Anne-Sophie; Burillon, Carole; Desanlis, Adeline; Damour, Odile; Kocaba, Viridiana; Auxenfans, Céline

    2016-06-01

    Descemet Membrane Endothelial Keratoplasty (DMEK) selectively replaces the damaged posterior part of the cornea. However, the DMEK technique relies on a manually-performed dissection that is time-consuming, requires training and presents a potential risk of endothelial graft damages leading to surgery postponement when performed by surgeons in the operative room. To validate precut corneal tissue preparation for DMEK provided by a cornea bank in order to supply a quality and security precut endothelial tissue. The protocol was a technology transfer from the Netherlands Institute for Innovative Ocular Surgery (NIIOS) to Lyon Cornea Bank, after formation in NIIOS to the DMEK "no touch" dissection technique. The technique has been validated in selected conditions (materials, microscope) and after a learning curve, cornea bank technicians prepared endothelial tissue for DMEK. Endothelial cells densities (ECD) were evaluated before and after preparation, after storage and transport to the surgery room. Microbiological and histological controls have been done. Twenty corneas were manually dissected; 18 without tears. Nineteen endothelial grafts formed a double roll. The ECD loss after cutting was 3.3 % (n = 19). After transportation 7 days later, we found an ECD loss of 25 % (n = 12). Three days after cutting and transportation, we found 2.1 % of ECD loss (n = 7). Histology found an endothelial cells monolayer lying on Descemet membrane. The mean thickness was 12 ± 2.2 µm (n = 4). No microbial contamination was found (n = 19). Endothelial roll stability has been validated at 3 days in our cornea bank. Cornea bank technicians trained can deliver to surgeons an ECD controlled, safety and ready to use endothelial tissue, for DMEK by "no touch" technique, allowing time saving, quality and security for surgeons. PMID:26934895

  10. Reflectivity of the human cornea and its influence on the selection of a suitable light source for a low-cost tonometer

    Science.gov (United States)

    Han, Yanmei; Bryanston-Cross, Peter J.; Shaw, Keith; Hero, Mark

    2002-09-01

    This article introduces an experiment designed to investigate which wavelength of light is best reflected by the cornea. Results indicate that the human cornea reflects near-infrared or violet light more efficiently than it does bright visible light. Further, results indicate that at a wavelength of 580nm, the reflectivity of the cornea decreases to a minimum value of 37% given light incident on the cornea at an angle of 45 degree. A light source suitable for the detection of glaucoma has been selected based on these results, also taking into account the spectral response of a suitable photo-detector.

  11. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    Science.gov (United States)

    Lee, Seunghun; Lee, Jun Ho; Park, Jin Hyoung; Yoon, Yeoreum; Chung, Wan Kyun; Tchah, Hungwon; Kim, Myoung Joon; Kim, Ki Hean

    2016-05-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. Intrinsic multiphoton fluorescence properties of moxifloxacin and gatifloxacin were characterized, and their distributions in mouse cornea in vivo were measured by 3D MPM imaging. Both moxifloxacin and gatifloxacin had similar multiphoton spectra, while moxifloxacin had stronger fluorescence than gatifloxacin. MPM imaging of mouse cornea in vivo showed (1) moxifloxacin had good penetration through the superficial corneal epithelium, while gatifloxacin had relatively poor penetration, (2) both ophthalmic solutions had high intracellular distribution. In vivo MPM results were consistent with previous studies. This study demonstrates the feasibility of MPM as a method for in vivo direct measurement of moxifloxacin and gatifloxacin in the cornea.

  12. Corneal Collagen Cross-Linking with Hypoosmolar Riboflavin Solution in Keratoconic Corneas

    Directory of Open Access Journals (Sweden)

    Shaofeng Gu

    2014-01-01

    Full Text Available Purpose. To report the 12-month outcomes of corneal collagen cross-linking (CXL with a hypoosmolar riboflavin and ultraviolet-A (UVA irradiation in thin corneas. Methods. Eight eyes underwent CXL using a hypoosmolar riboflavin solution after epithelial removal. The corrected distance visual acuity (CDVA, manifest refraction, the mean thinnest corneal thickness (MTCT, and the endothelial cell density (ECD were evaluated before and 6 and 12 months after CXL. Results. The MTCT was 413.9 ± 12.4 μm before treatment and reduced to 381.1 ± 7.3 μm after the removal of the epithelium. After CXL, the thickness decreased to 410.3 ± 14.5 μm at the last follow-up. Before treatment, the mean K-value of the apex of the keratoconus corneas was 58.7 ± 3.5 diopters and slightly decreased (57.7 ± 4.9 diopters at 12 months. The mean CDVA was 0.54 ± 0.23 logarithm of the minimal angle of resolution before treatment and increased to 0.51 ± 0.21 logarithm at the last follow-up. The ECD was 2731.4 ± 191.8 cells/mm2 before treatment and was 2733.4 ± 222.6 cells/mm2 at 12 months after treatment. Conclusions. CXL with a hypoosmolar riboflavin in thin corneas seems to be a promising method for keratoconic eyes with the mean thinnest corneal thickness less than 400 μm without epithelium.

  13. Excitation by irritant chemical substances of sensory afferent units in the cat's cornea.

    Science.gov (United States)

    Belmonte, C; Gallar, J; Pozo, M A; Rebollo, I

    1991-01-01

    1. Single-unit electrical activity was recorded from thin myelinated sensory nerve fibres innervating the cornea of deeply anaesthetized cats. 2. Based on their responses to mechanical (calibrated von Frey hairs), chemical (10 mM-acetic acid and/or 616 mM-NaCl) and thermal (ice-cold or heat up to 51 degrees C) stimuli, corneal A delta fibres were classified as polymodal nociceptors (63%), high-threshold mechanoceptors (22%) and mechano-heat nociceptors (15%). Thin myelinated fibres responding only to cold were found in the limbus of the eye. 3. Application of 10 mM-acetic acid on the corneal surface for 30 s evoked in polymodal fibres a brisk discharge of impulses often followed by a low-frequency impulse activity. NaCl (616 mM) produced a more gradual and sustained firing response. 4. The responses of polymodal fibres to acid were proportional to extracellular pH values (pH range: 4.5-6.0). After sensitization to repeated heating, most mechano-heat units developed a sensitivity to acidic stimulation. 5. Topical 0.33 mM-capsaicin excited polymodal nociceptors of the cornea; 5 min after capsaicin about 15% of these fibres were inactivated to all subsequent stimuli. In the rest of the fibres, chemical and thermal sensitivity disappeared after 0.33-3.3 mM-capsaicin, but mechanosensitivity was preserved. 6. Corneal mechanoceptors and limbal cold receptors were not affected by capsaicin (up to 33 mM). 7. These experiments demonstrate that the cornea of the cat is innervated by polymodal as well as mechanoceptive A delta nociceptors. In polymodal nociceptive fibres, mechanical and chemical sensitivities appear to be subserved by separate transduction mechanisms. PMID:1890657

  14. Effects of Er:YAG lasers of different pulse widths on rabbit corneas

    Science.gov (United States)

    Lian, Jing-cai; Wang, Kang-sun

    1995-05-01

    Photorefractive keratectomy with 193 nm excimer laser has been used for the treatment of myopia. Because of its big size and hazards of toxic gas, a 2.94 micrometers Er:YAG laser had been suggested for this purpose. The aims of the present study was to investigate the thermal effects in the corneas after exposed to Er:YAG laser with different pulse widths. 14 New- Zealand white rabbits were divided into two groups. In the first group, the corneas were irradiated by a normal spiking mode Er:YAG laser with a pulse width of 150 microsecond(s) at the fluence of 585 approximately 697 mJ/cm2. In the second group, the corneas were exposed to a Q-switch mode of Er:YAG laser with a pulse width of 700 ns at the fluence of 524 approximately 562 mJ/cm2 and 700 approximately 850 mJ/cm2. The eyes were followed up 5 months and enucleated for histopathologic inspection 30 minutes, 2 weeks, 1, 2 and 5 months postoperatively. Corneal stromal hazes in the first group were more obvious than that in the second group. The most significant haze was observed two weeks after treatments and disappeared at two months. The adjacent thermal damage in the first group were much wider than that in the second group. To minimize the thermal and shock wave effect, the Q-switch mode Er:YAG laser with pulse width around 700 ns is recommended for corneal surgery.

  15. Angular distribution of light emission from compound-eye cornea with conformal fluorescent coating

    Science.gov (United States)

    Martín-Palma, Raúl J.; Miller, Amy E.; Pulsifer, Drew P.; Lakhtakia, Akhlesh

    2014-09-01

    The complex morphology of the apposition compound eyes of insects of many species provides them a wide angular field of view. This characteristic makes these eyes attractive for bioreplication as artificial sources of light. The cornea of a blowfly eye was conformally coated with a fluorescent thin film with the aim of achieving wide field-of-view emission. On illumination by shortwave-ultraviolet light, the conformally coated eye emitted visible light whose intensity showed a weaker angular dependence than a fluorescent thin film deposited on a flat surface.

  16. Radiosensitivity of normal, regenerating and embryonic epithelium of the eye cornea in mice

    International Nuclear Information System (INIS)

    A quantitative cytogenetic study was performed with regard to the effect of X-rays on normal and regenerating epithelia of the eye cornea in adult and newborn C57Bl mice. The regenerating and embryonic epithelia were shown to be more stable than the normal epithelium in adult mice. This phenomenon was revealed in considerably shorter blocking of mitoses after irradiation and in lesser number of cells with chromosomal aberrations. The resultant data showed the radiosensitivity of the tissue with various functional states to have been determined by structural and metabolic features, rather than by proliferation intensity

  17. Proliferative activity of endotheliocytes of growing capillaries of the rabbit cornea

    Energy Technology Data Exchange (ETDEWEB)

    Gurina, O.Yu.; Mamontov, S.G.; Banin, V.V.

    1987-10-01

    The authors studied the intensity of DNA synthesis by cells of newly formed capillaries, growing in the rabbit cornea, after infliction of a silver nitrate burn and local application of colchicine. The intensity of capillary growth was investigated during stimulation and a combination of the burn with colchicine. Changes in activity of DNA synthesis by the endotheliocytes of newly formed capillaries during exposure throughout growth were also investigated. The intensity of cell proliferation was studied by measuring the incorporation of tritium-labelled thymidine into the endotheoiocyte nuclei.

  18. Comparison of Scheimpflug imaging parameters between steep and keratoconic corneas of Caucasian eyes

    Directory of Open Access Journals (Sweden)

    Huseynova T

    2016-04-01

    Full Text Available Tukezban Huseynova,1 Farah Abdulaliyeva,2 Michele Lanza3 1Briz-L Eye Clinic, 2National Ophthalmology Center, Baku, Azerbaijan; 3Second University of Naples, Caserta, Campania, Italy Purpose: To compare the keratometric and pachymetric parameters of healthy eyes with those affected by steep cornea and keratoconus (KC using Scheimpflug camera.Setting: Briz-L Eye Clinic, Baku, Azerbaijan.Design: A cross-sectional study.Methods: In this study, 49 KC (Amsler–Krumeich stage 1 eyes and 36 healthy eyes were enrolled. A complete ophthalmic evaluation and a Scheimpflug camera scan were performed in every eye included in the study. Tomographic parameters such as parameters from the front and back cornea, maximum keratometry reading (Kmax, corneal volume (CV, anterior chamber volume (ChV, anterior chamber depth (ACD, anterior chamber angle (AC angle, keratometric power deviation (KPD, maximum front elevation (Max FE, and maximum back elevation (Max BE, as well as pachymetric progression indices (PPI, Ambrosio relational thickness (ART, index of surface variance (ISV, index of vertical asymmetry (IVA, center keratoconus index (CKI, index of height asymmetry (IHA, index of height decentration (IHD, and radius minimum (RM were collected and statistically compared between the two groups.Results: PPI, ART, ISV, IVA, CKI, IHA, IHD, and RM parameter values were significantly different (P<0.05 between the KC and healthy eyes. There were no significant differences in K mean and Q values of the frontal corneal parameters, as well as in Kmax, AC angle, RM, back, and front astigmatism, between stage 1 keratoconic and normal Caucasian eyes with steep cornea. All other parameters such as K mean and Q values of the back corneal parameters, Max FE, Max BE, ACD, ChV, and CV showed significant differences between the groups (P<0.05 for all. Conclusion: Scheimpflug imaging is able to detect corneal morphological differences between stage 1 KC eyes and healthy eyes with

  19. Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

    OpenAIRE

    Hieronim Bartel; Dariusz Tosik; Monika Kujawa-Hadryś

    2010-01-01

    The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubati...

  20. Influence of the He-Ne laser in different irradiation regimes on the cornea cells after ionizing irradiation

    International Nuclear Information System (INIS)

    A study was made on epithelium cells of the eye cornea in mices entering the first postradiation mitosis. The influence of laser rays acting in the course of each exposure during one and the same period of time, or continuously, or by pulses was compared. The effect of laser radiation was evaluated from the frequency of occurrence of cells with chromosomal aberrations, mitotic index, coefficient of mitosis phases. It has been established that pulse radiation of a helium-neon laser in a certain operation regime produces a sanitating effect on cells of eye cornea epithelium in white mice damaged by ionizing radiation. Cells being killed in the interphase were not revealed in epithelium of the eye cornea. It enables to conclude that the amount of aberrant mitoses decreases as a result of postradiation repair of cells damaged by ionizing radiation, but not as a result of their elimination

  1. Preparation and in vitro characterization of BC/PVA hydrogel composite for its potential use as artificial cornea biomaterial

    Energy Technology Data Exchange (ETDEWEB)

    Wang Jiehua [School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072 (China); Gao Chuan; Zhang Yansen [School of Materials Science and Engineering, Tianjin University, Tianjin 300072 (China); Wan Yizao, E-mail: yzwantju@yahoo.com [School of Materials Science and Engineering, Tianjin University, Tianjin 300072 (China); Key Laboratory of Advanced Ceramics and Machining Technology, Ministry of Education, Tianjin University, Tianjin 300072 (China); Research Institute of Composite Materials, Tianjin University, Tianjin 300072 (China)

    2010-01-01

    In order to investigate the potential use for bacterial cellulose (BC) as a novel artificial cornea replacement, BC/poly(vinyl alcohol) (BC/PVA) hydrogel composites were synthesized by freezing-thaw method. The BC/PVA composites were characterized by UV-Vis spectrophotometer (UV-Vis), X-ray diffraction (XRD), thermogravimetric (TG) analysis, mechanical property tests and scanning electron microscope (SEM) analyses. Our results showed that the resultant BC/PVA composites exhibited desirable properties as artificial cornea replacement biomaterial including high water content, high visible light transmittance and suitable UV absorbance, increased mechanical strength and appropriate thermal properties. Results of this work revealed that the BC/PVA composites exhibited some promising characteristics as artificial cornea composite material and may be improved further for its realistic applications.

  2. X-ray scattering used to map the preferred collagen orientation in the human cornea and limbus.

    Science.gov (United States)

    Aghamohammadzadeh, Hossein; Newton, Richard H; Meek, Keith M

    2004-02-01

    Many properties of connective tissues are governed by the organization of the constituent collagen. For example, the organization of collagen in the cornea and the limbus, where the cornea and sclera meet, is an important determinant of corneal curvature and hence of the eye's focusing power. We have used synchrotron X-ray scattering to map the orientation of the collagen fibrils throughout the human cornea, limbus, and adjacent sclera. We demonstrate a preferred orientation of collagen in the vertical and horizontal directions that is maintained to within about 1 mm from the limbus, where a circular or tangential disposition of fibrils occurs. The data are also used to map the relative distribution of both the total and the preferentially aligned collagen in different parts of the tissue, revealing considerable anisotropy. The detailed structural information provided is an important step toward understanding the shape and the mechanical properties of the tissue. PMID:14962385

  3. Case report: a novel KERA mutation associated with cornea plana and its predicted effect on protein function

    DEFF Research Database (Denmark)

    Roos, Laura; Bertelsen, Birgitte; Harris, Pernille; Bygum, Anette; Jensen, Hanne; Grønskov, Karen; Tümer, Zeynep

    2015-01-01

    Background: Cornea plana (CNA) is a hereditary congenital abnormality of the cornea characterized by reduced corneal curvature, extreme hypermetropia, corneal clouding and hazy corneal limbus. The recessive form, CNA2, is associated with homozygous or compound heterozygous mutations of the...... keratocan gene (KERA) on chromosome 12q22. To date, only nine different disease-associated KERA mutations, including four missense mutations, have been described. Case presentation: In this report, we present clinical data from a Turkish family with autosomal recessive cornea plana. In some of the affected...... individuals, hypotrichosis was found. KERA was screened for mutations using Sanger sequencing. We detected a novel KERA variant, p.(Ile225Thr), that segregates with the disease in the homozygous form. The three-dimensional structure of keratocan protein was modelled, and we showed that this missense variation...

  4. Preparation and in vitro characterization of BC/PVA hydrogel composite for its potential use as artificial cornea biomaterial

    International Nuclear Information System (INIS)

    In order to investigate the potential use for bacterial cellulose (BC) as a novel artificial cornea replacement, BC/poly(vinyl alcohol) (BC/PVA) hydrogel composites were synthesized by freezing-thaw method. The BC/PVA composites were characterized by UV-Vis spectrophotometer (UV-Vis), X-ray diffraction (XRD), thermogravimetric (TG) analysis, mechanical property tests and scanning electron microscope (SEM) analyses. Our results showed that the resultant BC/PVA composites exhibited desirable properties as artificial cornea replacement biomaterial including high water content, high visible light transmittance and suitable UV absorbance, increased mechanical strength and appropriate thermal properties. Results of this work revealed that the BC/PVA composites exhibited some promising characteristics as artificial cornea composite material and may be improved further for its realistic applications.

  5. Assessing the viscoelasticity of green light induced CXL in the rabbit cornea by noncontact OCE and FEM

    Science.gov (United States)

    Han, Zhaolong; Li, Jiasong; Singh, Manmohan; Vantipalli, Srilatha; Aglyamov, Salavat R.; Wu, Chen; Liu, Chih-hao; Twa, Michael D.; Larin, Kirill V.

    2016-03-01

    The biomechanical properties of the cornea have a profound influence on its health and function. Rose bengal/green light corneal collagen cross-linking (RGX) has been proposed as an alternative to UV-A Riboflavin collagen cross-linking (UV-CXL) for treatment of keratoconus. However, the effects of RGX on the biomechanical properties of the cornea are not as well understood as UV-CXL. In this work, we demonstrate the feasibility of quantifying the viscoelasticity of the rabbit cornea before and after RGX using a noncontact method of phase-stabilized swept source optical coherence elastography (PhS-SSOCE) and finite element modeling (FEM). Viscoelastic FE models of the corneas were constructed to simulate the elastic wave propagation based on the OCE measurements. In addition, the effect of the fluid-structure interface (FSI) between the corneal posterior surface and aqueous humor on the elastic wave group velocity was also investigated. The effect of the FSI was first validated by OCE measurements and FEM simulations on contact lenses, and the OCE and FEM results were in good agreement. The Young's modulus of the rabbit cornea before RGX was assessed as E=80 kPa, and the shear viscosity was η=0.40 Pa•s at an intraocular pressure (IOP) of 15 mmHg. After RGX, the Young's modulus increased to E=112 kPa and shear viscosity decreased to η=0.37 Pa•s. Both the corneal OCE experiments and the FE simulations also demonstrated that the FSI significantly reduced the group velocity of the elastic wave, and thus, the FSI should be considered when determining the biomechanical properties of the cornea.

  6. Characterization of Ocular Iontophoretic Drug Transport of Ionic and Non-ionic Compounds in Isolated Rabbit Cornea and Conjunctiva.

    Science.gov (United States)

    Sekijima, Hidehisa; Ehara, Junya; Hanabata, Yusuke; Suzuki, Takumi; Kimura, Soichiro; Lee, Vincent H L; Morimoto, Yasunori; Ueda, Hideo

    2016-06-01

    Ocular iontophoresis (IP) in isolated rabbit cornea and conjunctiva was examined in terms of transport enhancement, tissue viability and integrity using electrophysiological parameters by the Ussing-type chamber technique. Lidocaine hydrochloride (LC, a cationic compound), sodium benzoate (BA, anionic compound), and fluorescein isothiocyanate labeled dextran (molecular weight 4400 Da, FD-4, hydrophilic large compound) were used as model permeants. Direct electric current was applied at 0.5-5.0 mA/cm(2) for the cornea and 0.5-20 mA/cm(2) for the conjunctiva for 30 min. LC and BA fluxes across the cornea and conjunctiva were significantly increased by the application of electric current up to 2.3- and 2.5-fold and 4.0- and 3.4-fold, respectively, and returned to their baseline level on stopping the current. Furthermore, a much higher increase by IP application was obtained for the FD-4 transport. The increased FD-4 flux in the conjunctiva returned to baseline on stopping the current, whereas the flux in the cornea was sustained at a higher level after stopping the current. The transepithelial electric resistance of the cornea and conjunctiva was lowered by electric current application but fully recovered after stopping the current up to 2.0 mA/cm(2) for the cornea and 10 mA/cm(2) for the conjunctiva, suggesting that the corneal and conjunctival viability and integrity are maintained even after application of these current densities. These results indicate that ocular IP may be a useful non-invasive technique to achieve drug delivery of hydrophilic large molecules into the eyes. PMID:27040754

  7. Nucleic acid-amplification testing for hepatitis B in cornea donors.

    Science.gov (United States)

    Fornés, Maria Gema; Jiménez, Maria Angustias; Eisman, Marcela; Gómez Villagrán, Jose Luis; Villalba, Rafael

    2016-06-01

    Careful donor selection and implementation of tests of appropriate sensitivity and specificity are of paramount importance for minimizing the risk of transmitting infectious diseases from donors to corneal allograft recipients. Reported cases of viral transmission with corneal grafts are very unusual. Nevertheless potential virus transmission through the engraftment cannot be ruled out. According to European Guideline 2006/17/EC, screening for antibodies for Hepatitis B core antigen (anti HBc) is mandatory, and when this test is positive, some criteria must be established before using corneas. Despite the continuous progress in screening tests, donors carrying an occult hepatitis B infection (OBI) can cause transplant-transmitted hepatitis B. To date, Nucleic Acid Testing (NAT) is not an obligatory assay in corneal tissue setting neither in our country nor in the rest of European countries. Herein, we report three cornea donors that were rejected with the diagnosis of OBI through the testing of sensitive NAT and the serological profile of Hepatitis B virus. The aim of this report is to emphasize the need to include NAT in new reviews of EU Tissues and Cells Directives in order to increase level of security in tissue donation as well as not to reject a high number of donors with isolated profile of anti HBc in geographical areas with high prevalence of Hepatitis B, that could be rejected without a true criterion of Hepatitis B infection. PMID:26685699

  8. Mass fabrication technique for polymeric replicas of arrays of insect corneas

    International Nuclear Information System (INIS)

    Motivated to develop a technique for producing many high-fidelity replicas for the sacrifice of a single biotemplate, we combined a modified version of the conformal-evaporated-film-by-rotation technique and electroforming to produce a master negative made of nickel from a composite biotemplate comprising several corneas of common blowflies. This master negative can function as either a mold for casting multiple replicas or a die for stamping multiple replicas. An approximately 250 nm thick nickel film was thermally deposited on an array of blowfly corneas to capture the surface features with high fidelity and then a roughly 60 μm thick structural layer of nickel was electroformed onto the thin layer to give it the structural integrity needed for casting or stamping. The master negative concurrently captured the spatial features of the biotemplate at length scales ranging from 200 nm to a few millimeters. Polymer replicas produced thereafter by casting did faithfully reproduce features of a few micrometers and larger in dimension.

  9. Control of Scar Tissue Formation in the Cornea: Strategies in Clinical and Corneal Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Samantha L. Wilson

    2012-09-01

    Full Text Available Corneal structure is highly organized and unified in architecture with structural and functional integration which mediates transparency and vision. Disease and injury are the second most common cause of blindness affecting over 10 million people worldwide. Ninety percent of blindness is permanent due to scarring and vascularization. Scarring caused via fibrotic cellular responses, heals the tissue, but fails to restore transparency. Controlling keratocyte activation and differentiation are key for the inhibition and prevention of fibrosis. Ophthalmic surgery techniques are continually developing to preserve and restore vision but corneal regression and scarring are often detrimental side effects and long term continuous follow up studies are lacking or discouraging. Appropriate corneal models may lead to a reduced need for corneal transplantation as presently there are insufficient numbers or suitable tissue to meet demand. Synthetic optical materials are under development for keratoprothesis although clinical use is limited due to implantation complications and high rejection rates. Tissue engineered corneas offer an alternative which more closely mimic the morphological, physiological and biomechanical properties of native corneas. However, replication of the native collagen fiber organization and retaining the phenotype of stromal cells which prevent scar-like tissue formation remains a challenge. Careful manipulation of culture environments are under investigation to determine a suitable environment that simulates native ECM organization and stimulates keratocyte migration and generation.

  10. First application of laser welding in clinical transplantation of the cornea

    Science.gov (United States)

    Pini, Roberto; Menabuoni, Luca; Starnotti, Lorenzo

    2001-05-01

    After a 4-year-long pre-clinical experimentation carried out at first on enucleated eyes and then on animal models, we applied a new procedure of laser welding of the cornea on voluntary patients. The welding technique is based on controlled irradiation of the cornea by diode laser radiation (805 nm) operating at low power (60-90 mW) in association with a photoenhancing chromophore applied locally. The welding effect is very effective and selective, because it takes place only in the cut where chromophore is present, while the contiguous tissue remains completely untouched. In the clinical phase, this technique was firstly tested in corneal cuts of increasing length on 25 patients subjected to facoemulsification of the cataract, by both sclero-corneal and corneal tunnels, and to extracapsular cataract extraction by sclero-corneal and corneal cuts. As previously confirmed by histological analysis performed on animal samples, we observed in humans too an early and effective healing process, with a sensible reduction of the post-operatory astigmatism. Based on these positive results, we finally arrived at the first application of diode laser- assisted corneal welding to penetrating keratoplasty (corneal transplantation), where this technique has been employed as far as now in 3 cases to substitute the application of the continuous suture.

  11. The ultrastructural alterations in rat corneas with experimentally-induced diabetes mellitus

    International Nuclear Information System (INIS)

    To examine the ultrastructural changes of rat corneas in streptozotocin (STZ) induced diabetes mellitus and the and the follow-up insulin treatment. Sprague-Dawley type rats were used for experimental procedures during the period from January to April 2003 at Baskent University, Ankara, Turkey. Rats were studied in four groups: group 1: controls, group 2 sham controls (single dose IV sodium citrate); group 3 STZ-induced diabetes mellitus (Single dose 45mg/kg STZ intravenously), group 4: diabetes mellitus + insulin treatment (8U/day). We observed degenerative changes in the epithelial layer, stromal keratocytes and endothelial cells in diabetic group. In contrast, the corneal layers have revealed positive alterations in the insulin-treated group. The statistical analysis, showed significant narrowing in the epithelial layer in the diabetic group (p0.02), whereas thickening was observed in the epithelial basement membrane and Descemet's membrane (p=0.002). It was determined that that diabetes mellitus causes degenerative changes in cornea, which are positively influenced by short-term insulin treatment. (author)

  12. The Effect of Actinoquinol with Hyaluronic Acid in Eye Drops on the Optical Properties and Oxidative Damage of the Rabbit Cornea Irradiated with UVB Rays

    Czech Academy of Sciences Publication Activity Database

    Čejka, Čestmír; Luyckx, J.; Ardan, Taras; Pláteník, J.; Širc, Jakub; Michálek, Jiří; Čejková, Jitka

    2010-01-01

    Roč. 86, č. 6 (2010), s. 1294-1306. ISSN 0031-8655 Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z40500505 Keywords : cornea * UVB-irradiated cornea * UV rays Subject RIV: FF - HEENT, Dentistry Impact factor: 2.679, year: 2010

  13. An active artificial cornea with the function of inducing new corneal tissue generation in vivo-a new approach to corneal tissue engineering

    International Nuclear Information System (INIS)

    An active artificial cornea which can perform the function of inducing new cornea generation in vivo but does not need culture cells in vitro and which has similar optical and mechanical properties to those of the human cornea was constructed. An animal keratoplasty experiment using the artificial cornea as the implant showed that the animals' corneas could keep smooth surface and clear stroma postoperatively, and that the repopulation of the host's keratocytes, the degradation of the implant and new corneal tissue generation were completed at 5-6 months after surgery. Such an artificial cornea has several advantages over other corneal equivalents constructed in the typical way of tissue engineering: in having similar mechanical and optical properties to those of the human cornea and with no exogenetic cells, it can be used universally in different implantation surgeries without immunoreaction; it is easy to prepare and process into different shapes and sizes on a large scale, and suitable for long-distance transportation and long-term storage. All these characteristics make it a new approach to cornea tissue engineering having potential in many clinical applications

  14. Corneal collagen crosslinking in keratoconus and other eye disease

    Institute of Scientific and Technical Information of China (English)

    Adel; Alhayek; Pei-Rong; Lu

    2015-01-01

    Keratoconus is a condition characterized by biomechanical instability of the cornea, presenting in a progressive, asymmetric and bilateral way. Corneal collagen crosslinking(CXL) with riboflavin and Ultraviolet-A(UVA) is a new technique of corneal tissue strengthening that combines the use of riboflavin as a photo sensitizer and UVA irradiation. Studies showed that CXL was effective in halting the progression of keratoconus over a period of up to four years. The published studies also revealed a reduction of max K readings by more than 2 D, while the postoperative spherical equivalent(SEQ) was reduced by an average of more than 1 D and refractive cylinder decreased by about1 D. The major indication for the use of CXL is to inhibit the progression of corneal ecstasies, such as keratoconus and pellucid marginal degeneration. CXL may also be effective in the treatment and prophylaxis of iatrogenic keratectasia, resulting from excessively aggressive photo ablation. This treatment has been used to treat infectious corneal ulcers with apparent favorable results. Most recent studies demonstrate the beneficial impact of CXL for iatrogenic ecstasies, pellucid marginal degeneration, infectious keratitis, bullous keratopathy and ulcerative keratitis. Several long-term and short-term complications of CXL have been studied and documented. The possibility of a secondary infection after the procedure exists because the patient is subject to epithelial debridement and the application of a soft contact lens. Formation of temporary corneal haze,permanent scars, endothelial damage, treatment failure,sterile infiltrates, bullous keratopathy and herpes reactivation are the other reported complications of this procedure.

  15. Global and local contributions to surface curva- ture of healthy corneas

    Directory of Open Access Journals (Sweden)

    Alan Rubin

    2012-12-01

    Full Text Available This paper demonstrates for several healthy eyes the application of a simple model to understanding local and global contributions to short-term variation in anterior and posterior corneal curvature. Multiple axial anterior and posterior corneal radii and central corneal thicknesses for the right eyes of 10 young subjects were determined over time using a rotating Scheimpflug camera (Oculus Pentacam. The axial radii were transformed to corneal powers, and also to curvatures that were referred to a mid-corneal surface such that local and global contributions to short-term variation could be analyzed quantitatively.When variation of the anterior and posterior corneal surfaces of several healthy eyes are studied in terms of curvatures (rather than powers it is the posterior surfaces that are more variable withthe global or macroscopic rather than local effects dominating. (Harris and Gillan found the same for an eye with mild keratoconus. This finding is opposite to that when variation is considered in terms of dioptric power where the anterior corneal surface usually appears more variable. Possible reasons for this finding includes firstly that the posterior corneal surface has to be measured through the air-tear interface and anterior corneal surface,and thus some uncertainty in measurements of the posterior surface may relate to this limitation. Secondly, no attempt was made here to mathematically align the multiple surfaces as determined per eye and thus we cannot be certain that precisely the same central corneal region was measured each time.Investigators need to carefully consider whether they are more interested in the optical or physical nature of variation in surfaces such as the cornea since studies of the optical effects require theanalysis to be performed in terms of dioptric powers and  symmetric dioptric power space whereas studies of physical variation in the topography of the cornea and the possible reasons for such variability

  16. Modeling of ultrashort pulsed laser irradiation in the cornea based on parabolic and hyperbolic heat equations using electrical analogy

    Science.gov (United States)

    Gheitaghy, A. M.; Takabi, B.; Alizadeh, M.

    2014-03-01

    Hyperbolic and parabolic heat equations are formulated to study a nonperfused homogeneous transparent cornea irradiated by high power and ultrashort pulsed laser in the Laser Thermo Keratoplasty (LTK) surgery. Energy absorption inside the cornea is modeled using the Beer-Lambert law that is incorporated as an exponentially decaying heat source. The hyperbolic and parabolic bioheat models of the tissue were solved by exploiting the mathematical analogy between thermal and electrical systems, by using robust circuit simulation program called Hspice to get the solutions of simultaneous RLC and RC transmission line networks. This method can be used to rapidly calculate the temperature in laser-irradiated tissue at time and space domain. It is found that internal energy gained from the irradiated field results in a rapid rise of temperature in the cornea surface during the early heating period, while the hyperbolic wave model predicts a higher temperature rise than the classical heat diffusion model. In addition, this paper investigates and examines the effect of some critical parameters such as relaxation time, convection coefficient, radiation, tear evaporation and variable thermal conductivity of cornea. Accordingly, it is found that a better accordance between hyperbolic and parabolic models will be achieved by time.

  17. Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

    International Nuclear Information System (INIS)

    A fibre optic spectrophotometer front-end system for measuring corneas to overcome shortcomings associated with existing instruments was tested. The system allowed prompt measurement postmortem, minimizing beam pathlength to reduce the effects of scatter and unwanted refraction and eliminated optical interfaces and cuvette media. Rabbit corneas were excised immediately postmortem and placed on a detecting fibre optic coupled to an Ocean Optics spectrophotometer and illuminated by a deuterium–halogen source. The compact instrument with its small beam size allowed tissue profiling at test points across the corneal surface and efficient interchange for comparison of different tissues. This simplified system operation allowed rapid tissue altering to study induced changes on transmittance. The corneal transmittance data showed a consistent sharp cut-off at 320 nm in the ultraviolet radiation (UVR) spectrum, which decayed rapidly from postmortem swelling. Inter- and intra-corneal consistency was demonstrated by comparing data from different regions of the same cornea and those from opposite eyes. Changes to the spectra, particularly in the UVB below 300 nm, were evident when the corneal epithelium was removed, indicating that this layer is not the only corneal UVR filter. The new system reduced much of the variability associated with previous methods, as it rapidly measured corneal transmittance postmortem. Data are in broad agreement with published transmittance curves. The removal of the corneal epithelium revealed a substantial stromal contribution to the overall corneal UVR absorption, suggesting that corneas with pathologically or iatrogenically thinned stromas are less effective UVR blockers

  18. Collection, processing and testing of bone, corneas, umbilical cord blood and haematopoietic stem cells by European Blood Alliance members

    DEFF Research Database (Denmark)

    Närhi, M; Natri, O; Desbois, I;

    2013-01-01

    A questionnaire study was carried out in collaboration with the European Blood Alliance (EBA) Tissues and Cells (T&C) working group. The aim was to assess the level of involvement and commonality of processes on the procurement, testing and storage of bone, corneas, umbilical cord blood (UCB) and...

  19. A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome

    DEFF Research Database (Denmark)

    Porter, Louise F; Galli, Giorgio G; Williamson, Sally;

    2015-01-01

    Type 2 brittle cornea syndrome (BCS2) is an inherited connective tissue disease with a devastating ocular phenotype caused by mutations in the transcription factor PRDM5 hypothesised to exert epigenetic effects through histone and DNA methylation. Here we investigate clinical samples, including s...

  20. Non-invasive sensor for determining functional characteristics of the cornea, device including said sensor and use thereof

    OpenAIRE

    Guimera Brunet, Antoni; Villa Sanz, Rosa; Gabriel Buguna, Gemma; Maldonado, Miguel José

    2010-01-01

    [EN] The invention relates to a sensor and a device including said sensor for non-invasively obtaining data that can be used to detennine the functional characteristics of the cornea, in particular to establish a correlation between the impedance to different frequencies and the penneability of the endothelium and the epithelium and the hydration level ofthe stroma.

  1. Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications

    Directory of Open Access Journals (Sweden)

    Andrew Hopkinson

    2013-07-01

    Full Text Available The worldwide limited availability of suitable corneal donor tissue has led to the development of alternatives, including keratoprostheses (Kpros and tissue engineered (TE constructs. Despite advances in bioscaffold design, there is yet to be a corneal equivalent that effectively mimics both the native tissue ultrastructure and biomechanical properties. Human decellularized corneas (DCs could offer a safe, sustainable source of corneal tissue, increasing the donor pool and potentially reducing the risk of immune rejection after corneal graft surgery. Appropriate, human-specific, decellularization techniques and high-resolution, non-destructive analysis systems are required to ensure reproducible outputs can be achieved. If robust treatment and characterization processes can be developed, DCs could offer a supplement to the donor corneal pool, alongside superior cell culture systems for pharmacology, toxicology and drug discovery studies.

  2. Keeping an eye on decellularized corneas: a review of methods, characterization and applications.

    Science.gov (United States)

    Wilson, Samantha L; Sidney, Laura E; Dunphy, Siobhán E; Rose, James B; Hopkinson, Andrew

    2013-01-01

    The worldwide limited availability of suitable corneal donor tissue has led to the development of alternatives, including keratoprostheses (Kpros) and tissue engineered (TE) constructs. Despite advances in bioscaffold design, there is yet to be a corneal equivalent that effectively mimics both the native tissue ultrastructure and biomechanical properties. Human decellularized corneas (DCs) could offer a safe, sustainable source of corneal tissue, increasing the donor pool and potentially reducing the risk of immune rejection after corneal graft surgery. Appropriate, human-specific, decellularization techniques and high-resolution, non-destructive analysis systems are required to ensure reproducible outputs can be achieved. If robust treatment and characterization processes can be developed, DCs could offer a supplement to the donor corneal pool, alongside superior cell culture systems for pharmacology, toxicology and drug discovery studies. PMID:24956084

  3. Collagen Cross-linking in Keratoconus Patients with Thin Corneas: Short-Term Results

    Directory of Open Access Journals (Sweden)

    Nurullah Çağıl

    2012-10-01

    Full Text Available Pur po se: To study the effectiveness and safety of corneal collagen cross-linking with hypoosmolar riboflavin solution applied to keratoconus patients with thin corneas. Ma te ri al and Met hod: In this retrospective study, medical records of keratoconus patients planned for cross-linking surgery and having thinnest corneal thickness (TCT less than 400 μm after corneal epithelial removal were reviewed. There were 12 patients and 16 eyes in the study cohort. After the epithelium was removed, hypoosmolar solution was applied for 30 minutes and pachymetric measurements were taken. If corneal thickness became more than 400 μm, the cross-linking procedure was started; if not, hypoosmolar solution was continued until corneal thickness reached 400 μm. Maximum keratometry values (K Max, pachymetric measurements, uncorrected distance visual acuities (UDVA, and corrected distance visual acuities (CDVA were recorded. Comparison between preoperative measurements and measurements taken in sixth postoperative month were performed. Re sults: The mean TCT was 422.75±26.98 μm preoperatively (max: 450, min: 360. The mean TCT was reduced to 373.63±22.41 μm after epithelium was removed (max: 398, min: 325. There was a statistically significant difference between preoperative K max (62.62±5.09 and postoperative K max (61.55±5.80, (p=0.03. On the other hand, the difference between preoperative-postoperative UDVA (p=0.29 and preoperative-postoperative CDVA was not significant (p=058. There were no cases with significant corneal opacity or with any other complication. Dis cus si on: Corneal collagen cross-linking with hypoosmolar riboflavin solution in keratoconus patients with thin corneas is an effective procedure and can be considered as safe regarding preservation of visual acuities and absence of significant corneal opacity. (Turk J Ophthalmol 2012; 42: 316-20

  4. Amniotic membrane covering promotes healing of cornea epithelium and improves visual acuity after debridement for fungal keratitis

    Institute of Scientific and Technical Information of China (English)

    Bo; Zeng; Ping; Wang; Ling-Juan; Xu; Xin-Yu; Li; Hong; Zhang; Gui-Gang; Li

    2014-01-01

    AIM:To investigate the effect of amniotic membrane covering(AMC) on the healing of cornea epithelium and visual acuity for fungal keratitis after debridement.METHODS:Twenty fungal keratitis patients were divided into two groups randomly, the AMC group and the control group, ten patients each group. Both debridement of the infected cornea tissue and standard anti-fungus drugs treatments were given to every patients, monolayer amniotic membrane were sutured to the surface of the entire cornea and bulbar conjunctiva with 10-0 nylon suture for patients in the AMC group.The diameter of the ulcer was determined with slit lamp microscope and the depth of the infiltration was determined with anterior segment optical coherence tomography. Uncorrected visual acuity(UCVA) was tested before surgery and three month after healing of the epithelial layer. The healing time of the cornea epithelium, visual acuity(VA) was compared between the two groups using t- test.RESULTS:There was no statistical difference of the diameter of the ulcer, depth of the infiltration, height of the hypopyon and VA between the two groups beforesurgery(P >0.05). The average healing time of the AMC group was 6.89 ±2.98 d, which was statistically shorter than that of the control group(10.23±2.78d)(P <0.05).The average UCVA of the AMC group was 0.138 ±0.083,which was statistically better than that of the control group(0.053±0.068)(P <0.05).CONCLUSION:AMC surgery could promote healing of cornea epithelium after debridement for fungal keratitis and lead to better VA outcome.

  5. Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas.

    Science.gov (United States)

    Yan, Chenxi; Gao, Nan; Sun, Haijing; Yin, Jia; Lee, Patrick; Zhou, Li; Fan, Xianqun; Yu, Fu-Shin

    2016-06-01

    Patients with diabetes mellitus often develop corneal complications and delayed wound healing. How diabetes might alter acute inflammatory responses to tissue injury, leading to delayed wound healing, remains mostly elusive. Using a streptozotocin-induced type I diabetes mellitus mice and corneal epithelium-debridement wound model, we discovered that although wounding induced marked expression of IL-1β and the secreted form of IL-1 receptor antagonist (sIL-1Ra), diabetes suppressed the expressions of sIL-1Ra but not IL-1β in healing epithelia and both in whole cornea. In normoglycemic mice, IL-1β or sIL-1Ra blockade delayed wound healing and influenced each other's expression. In diabetic mice, in addition to delayed reepithelization, diabetes weakened phosphatidylinositol 3-kinase-Akt signaling, caused cell apoptosis, diminished cell proliferation, suppressed neutrophil and natural killer cell infiltrations, and impaired sensory nerve reinnervation in healing mouse corneas. Local administration of recombinant IL-1Ra partially, but significantly, reversed these pathological changes in the diabetic corneas. CXCL10 was a downstream chemokine of IL-1β-IL-1Ra, and exogenous CXCL10 alleviated delayed wound healing in the diabetic, but attenuated it in the normal corneas. In conclusion, the suppressed early innate/inflammatory responses instigated by the imbalance between IL-1β and IL-1Ra is an underlying cause for delayed wound healing in the diabetic corneas. Local application of IL-1Ra accelerates reepithelialization and may be used to treat chronic corneal and potential skin wounds of diabetic patients. PMID:27109611

  6. A comparison study of Riboflavin/UV-A and Rose-Bengal/Green light cross-linking of the rabbit corneas using optical coherence elastography

    Science.gov (United States)

    Li, Jiasong; Singh, Manmohan; Han, Zhaolong; Vantipalli, Srilatha; Liu, Chih-Hao; Wu, Chen; Raghunathan, Raksha; Kazemi, Tina; Twa, Michael D.; Larin, Kirill V.

    2016-03-01

    The biomechanical properties of the cornea are critical factors which determine its health and subsequent visual acuity. Keratoconus is a structural degeneration of the cornea which can diminish vision quality. Riboflavin/UV-A corneal collagen cross-linking (UV-CXL) is an emerging treatment that increases the stiffness of the cornea and improves its ability to resist further degeneration. While UV-CXL has shown great promise for effective therapy of the keratoconus, there are concerns associated with the UV irradiation, such as keratocyte cytotoxicity. Rose-bengal/green light corneal collagen cross-linking (RGX) has been proposed as an alternative to UV-CXL. Because of the high absorbance of the rose-bengal dye at green wavelengths, the treatment time is significantly shorter than with UV-CXL. Moreover, because green light is used in lieu of UV irradiation, there are no cytotoxic side-effects. In this study, noncontact optical coherence elastography (OCE) was used to compare the outcomes of UV-CXL and RGX treatment in rabbit cornea. Low-amplitude (micrometer scale) elastic waves were induced by a focused air-pulse loading system. The elastic wave propagation was then imaged by a phase-stabilized swept source OCE (PhS-SSOCE) system. The changes in the viscoelasticity of the corneas were quantified by a previously developed modified Rayleigh Lamb frequency model. The depth-resolved micro-scale phase-velocity distribution in the cornea was used to reveal the depth-wise heterogeneity before and after both cross-linking techniques. Our results show that UV-CXL and RGX increased the stiffness of the corneas by ~54% and ~5% while reducing the viscosity by ~42% and ~17%, respectively. The depth-wise phase velocities showed that UV-CXL affected the anterior ~1/3 of the corneas, while RGX only affected the anterior ~1/7 of the corneas.

  7. Novel micelle carriers for cyclosporin A topical ocular delivery: in vivo cornea penetration, ocular distribution and efficacy studies.

    Science.gov (United States)

    Di Tommaso, Claudia; Bourges, Jean-Louis; Valamanesh, Fatemeh; Trubitsyn, Gregory; Torriglia, Alicia; Jeanny, Jean-Claude; Behar-Cohen, Francine; Gurny, Robert; Möller, Michael

    2012-06-01

    Cornea transplantation is one of the most performed graft procedures worldwide with an impressive success rate of 90%. However, for "high-risk" patients with particular ocular diseases in addition to the required surgery, the success rate is drastically reduced to 50%. In these cases, cyclosporin A (CsA) is frequently used to prevent the cornea rejection by a systemic treatment with possible systemic side effects for the patients. To overcome these problems, it is a challenge to prepare well-tolerated topical CsA formulations. Normally high amounts of oils or surfactants are needed for the solubilization of the very hydrophobic CsA. Furthermore, it is in general difficult to obtain ocular therapeutic drug levels with topical instillations due to the corneal barriers that efficiently protect the intraocular structures from foreign substances thus also from drugs. The aim of this study was to investigate in vivo the effects of a novel CsA topical aqueous formulation. This formulation was based on nanosized polymeric micelles as drug carriers. An established rat model for the prevention of cornea graft rejection after a keratoplasty procedure was used. After instillation of the novel formulation with fluorescent labeled micelles, confocal analysis of flat-mounted corneas clearly showed that the nanosized carriers were able to penetrate into all corneal layers. The efficacy of a 0.5% CsA micelle formulation was tested and compared to a physiological saline solution and to a systemic administration of CsA. In our studies, the topical CsA treatment was carried out for 14 days, and the three parameters (a) cornea transparency, (b) edema, and (c) neovascularization were evaluated by clinical observation and scoring. Compared to the control group, the treated group showed a significant higher cornea transparency and significant lower edema after 7 and 13 days of the surgery. At the end point of the study, the neovascularization was reduced by 50% in the CsA-micelle treated

  8. Social influence of a religious hero: the late Cardinal Stephen Kim Sou-hwan's effect on cornea donation and volunteerism.

    Science.gov (United States)

    Bae, Hyuhn-Suhck; Brown, William J; Kang, Seok

    2011-01-01

    This study examined the mediated influence of a celebrated religious hero in South Korea, Cardinal Stephen Kim, through two forms of involvement--parasocial interaction and identification--on intention toward cornea donation and volunteerism, and it investigated how the news media diffused of his death. A structural equation modeling analysis with a Web-based voluntary survey of more than 1,200 people in South Korea revealed a multistep social influence process, beginning with parasocial interaction with Cardinal Kim, leading to identification with him, which predicted intention toward cornea donation and volunteerism. Additional investigations found that news of Cardinal Kim's death diffused rapidly through media and interpersonal communication. Results of this study demonstrate that religious leaders who achieve a celebrity hero status can prompt public discussion of important issues rather quickly through extensive media coverage, enabling them to promote prosocial behavior and positively affect public health. PMID:21086210

  9. Oxidative Stress to the Cornea, Changes in Corneal Optical Properties, and Advances in Treatment of Corneal Oxidative Injuries

    Directory of Open Access Journals (Sweden)

    Cestmir Cejka

    2015-01-01

    Full Text Available Oxidative stress is involved in many ocular diseases and injuries. The imbalance between oxidants and antioxidants in favour of oxidants (oxidative stress leads to the damage and may be highly involved in ocular aging processes. The anterior eye segment and mainly the cornea are directly exposed to noxae of external environment, such as air pollution, radiation, cigarette smoke, vapors or gases from household cleaning products, chemical burns from splashes of industrial chemicals, and danger from potential oxidative damage evoked by them. Oxidative stress may initiate or develop ocular injury resulting in decreased visual acuity or even vision loss. The role of oxidative stress in the pathogenesis of ocular diseases with particular attention to oxidative stress in the cornea and changes in corneal optical properties are discussed. Advances in the treatment of corneal oxidative injuries or diseases are shown.

  10. Oxidative stress to the cornea, changes in corneal optical properties, and advances in treatment of corneal oxidative injuries.

    Science.gov (United States)

    Cejka, Cestmir; Cejkova, Jitka

    2015-01-01

    Oxidative stress is involved in many ocular diseases and injuries. The imbalance between oxidants and antioxidants in favour of oxidants (oxidative stress) leads to the damage and may be highly involved in ocular aging processes. The anterior eye segment and mainly the cornea are directly exposed to noxae of external environment, such as air pollution, radiation, cigarette smoke, vapors or gases from household cleaning products, chemical burns from splashes of industrial chemicals, and danger from potential oxidative damage evoked by them. Oxidative stress may initiate or develop ocular injury resulting in decreased visual acuity or even vision loss. The role of oxidative stress in the pathogenesis of ocular diseases with particular attention to oxidative stress in the cornea and changes in corneal optical properties are discussed. Advances in the treatment of corneal oxidative injuries or diseases are shown. PMID:25861412

  11. The Resin-Embedded Cornea Prepared Via Rapid Processing Protocol : A Good Histomorphometric Target for Clinical Investigation in Ophthalmology and Optometry

    OpenAIRE

    Cheah, Pike See; Mohidin, Norhani; Mohd Ali, Bariah; Maung, Myint; Latif, Azian Abdul

    2008-01-01

    This study illustrates and quantifies the changes on corneal tissue between the paraffin-embedded and resin-embedded blocks and thus, selects a better target in investigational ophthalmology and optometry via light microscopy. Corneas of two cynomolgus monkeys (Macaca fascicularis) were used in this study. The formalin-fixed cornea was prepared in paraffin block via the conventional tissue processing protocol (4-day protocol) and stained with haematoxylin and eosin. The glutaraldehyde-fixed c...

  12. Oxidative Stress to the Cornea, Changes in Corneal Optical Properties, and Advances in Treatment of Corneal Oxidative Injuries

    Czech Academy of Sciences Publication Activity Database

    Čejka, Čestmír; Čejková, Jitka

    2015-01-01

    Roč. 2015, Mar 11 (2015), s. 591530. ISSN 1942-0900 R&D Projects: GA ČR(CZ) GA14-12580S Keywords : experimental autoimmune uveitis * eye sjogrens-syndrome * rabbit cornea * aldehyde dehydrogenase * uvb rays * glutathione-peroxidase * superoxide-dismutase * refractive-index * reactive oxygen * alkali-burn Subject RIV: FF - HEENT, Dentistry Impact factor: 3.516, year: 2014

  13. Unilateral evisceration of an eye following cornea and lens perforation in a sulfur-crested cockatoo (Cacatua galerita)

    OpenAIRE

    CHRISTEN, C.; Richter, M.; Fischer, I.; Eule, C; Spiess, B M; Hatt, J M

    2006-01-01

    A 24-year old male sulfur-crested Cockatoo (Cacatua galerita) was presented with a subacute perforation of the cornea without involvement of the lens.The bird was treated conservatively and the eye remained quiescent up to a second traumatic corneal perforation associated with a lens capsule rupture 15 months later. Due to the second perforating trauma of an already blind eye involving the lens, evisceration of the eye was performed. Two months after surgery the cosmetic result was excellent....

  14. Oxidative Stress to the Cornea, Changes in Corneal Optical Properties, and Advances in Treatment of Corneal Oxidative Injuries

    OpenAIRE

    Cestmir Cejka; Jitka Cejkova

    2015-01-01

    Oxidative stress is involved in many ocular diseases and injuries. The imbalance between oxidants and antioxidants in favour of oxidants (oxidative stress) leads to the damage and may be highly involved in ocular aging processes. The anterior eye segment and mainly the cornea are directly exposed to noxae of external environment, such as air pollution, radiation, cigarette smoke, vapors or gases from household cleaning products, chemical burns from splashes of industrial chemicals, and danger...

  15. Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium.

    Science.gov (United States)

    Miyashita, Hideyuki; Shimmura, Shigeto; Kobayashi, Hisatoshi; Taguchi, Tetsushi; Asano-Kato, Naoko; Uchino, Yuichi; Kato, Masabumi; Shimazaki, Jun; Tanaka, Junzo; Tsubota, Kazuo

    2006-01-01

    The cornea is a transparent tissue of the eye, which is responsible for the refraction of incoming light. Both biological corneal equivalents and synthetic keratoprostheses have been developed to replace donor tissue as a means to restore vision. However, both designs have drawbacks in terms of stability and biocompatibility. Clinically available synthetic devices do not support an intact epithelium, which poses a risk of microbial infection or protrusion of the prosthesis. In the present study, type I collagen was immobilized onto poly(vinyl alcohol) (PVA-COL) as a possible artificial cornea scaffold that can sustain a functional corneal epithelium. Human and rabbit corneal epithelial cells were air-lift cultured with 3T3 feeder fibroblasts to form a stratified epithelial layer on PVA-COL. The epithelial sheet expressed keratin 3/12 differentiation markers, the tight junction protein occludin, and had characteristic microvilli structures on transmission electron microscopy. Functionally, the stratified epithelium contained normal glycogen levels, and an apical tight-junction network was observed to exclude the diffusion of horseradish peroxidase. Furthermore, the epithelium-PVA-COL composite was suturable in the rabbit cornea, suggesting the possibility of using PVA-COL as a biocompatible material for keratoprosthesis. PMID:16044431

  16. Transdifferentiation from cornea to lens in Xenopus laevis depends on BMP signalling and involves upregulation of Wnt signalling

    Directory of Open Access Journals (Sweden)

    Day Robert C

    2011-09-01

    Full Text Available Abstract Background Surgical removal of the lens from larval Xenopus laevis results in a rapid transdifferention of central corneal cells to form a new lens. The trigger for this process is understood to be an induction event arising from the unprecedented exposure of the cornea to the vitreous humour that occurs following lens removal. The molecular identity of this trigger is unknown. Results Here, we have used a functional transgenic approach to show that BMP signalling is required for lens regeneration and a microarray approach to identify genes that are upregulated specifically during this process. Analysis of the array data strongly implicates Wnt signalling and the Pitx family of transcription factors in the process of cornea to lens transdifferentiation. Our analysis also captured several genes associated with congenital cataract in humans. Pluripotency genes, in contrast, were not upregulated, supporting the idea that corneal cells transdifferentiate without returning to a stem cell state. Several genes from the array were expressed in the forming lens during embryogenesis. One of these, Nipsnap1, is a known direct target of BMP signalling. Conclusions Our results strongly implicate the developmental Wnt and BMP signalling pathways in the process of cornea to lens transdifferentiation (CLT in Xenopus, and suggest direct transdifferentiation between these two anterior eye tissues.

  17. Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Glucaric cornea

    International Nuclear Information System (INIS)

    The composition, structure and rheological properties of soluble sulphated polysaccharide Glucaric cornea from Brazilian red seaweeds were investigated. The main components of polysaccharide were 3,6-anhydrogalactose (24.7%) and galactose (64.6%). In addition, minor components as 6-O-methyl-galactose (8.5%), glucose (1.5%), xylose (0.7%) and sulfated groups (4.8%) were detected. Comparison between sulphates content determined by Ft-IR spectroscopy and micro elemental analysis was made. Data from 13C NMR and FT-IR provided evidence of sulphation in C-4 and C-6 of galactose. No gelation with 1.5, 2.0 and 3.0 % (w/v) aqueous solution was observed, even cooled up to 4 deg C. GPC indicated two majors polysaccharide fractions of Mpk 7.4 x 104 and 1.8 x 104 g/mol and a minor fraction of Mpk 2.1 x 106 g/mol. (author)

  18. Matrix metalloproteinase 14 modulates signal transduction and angiogenesis in the cornea.

    Science.gov (United States)

    Chang, Jin-Hong; Huang, Yu-Hui; Cunningham, Christy M; Han, Kyu-Yeon; Chang, Michael; Seiki, Motoharu; Zhou, Zhongjun; Azar, Dimitri T

    2016-01-01

    The cornea is transparent and avascular, and retention of these characteristics is critical to maintaining vision clarity. Under normal conditions, wound healing in response to corneal injury occurs without the formation of new blood vessels; however, neovascularization may be induced during corneal wound healing when the balance between proangiogenic and antiangiogenic mediators is disrupted to favor angiogenesis. Matrix metalloproteinases (MMPs), which are key factors in extracellular matrix remodeling and angiogenesis, contribute to the maintenance of this balance, and in pathologic instances, can contribute to its disruption. Here, we elaborate on the facilitative role of MMPs, specifically MMP-14, in corneal neovascularization. MMP-14 is a transmembrane MMP that is critically involved in extracellular matrix proteolysis, exosome transport, and cellular migration and invasion, processes that are critical for angiogenesis. To aid in developing efficacious therapies that promote healing without neovascularization, it is important to understand and further investigate the complex pathways related to MMP-14 signaling, which can also involve vascular endothelial growth factor, basic fibroblast growth factor, Wnt/β-catenin, transforming growth factor, platelet-derived growth factor, hepatocyte growth factor or chemokines, epidermal growth factor, prostaglandin E2, thrombin, integrins, Notch, Toll-like receptors, PI3k/Akt, Src, RhoA/RhoA kinase, and extracellular signal-related kinase. The involvement and potential contribution of these signaling molecules or proteins in neovascularization are the focus of the present review. PMID:26647161

  19. Advancement in polarimetric glucose sensing: simulation and measurement of birefringence properties of cornea

    Science.gov (United States)

    Malik, Bilal H.; Coté, Gerard L.

    2011-03-01

    Clinical guidelines dictate that frequent blood glucose monitoring in diabetic patients is critical towards proper management of the disease. Although, several different types of glucose monitors are now commercially available, most of these devices are invasive, thereby adversely affecting patient compliance. To this end, optical polarimetric glucose sensing through the eye has been proposed as a potential noninvasive means to aid in the control of diabetes. Arguably, the most critical and limiting factor towards successful application of such a technique is the time varying corneal birefringence due to eye motion artifact. We present a spatially variant uniaxial eye model to serve as a tool towards better understanding of the cornea's birefringence properties. The simulations show that index-unmatched coupling of light is spatially limited to a smaller range when compared to the index-matched situation. Polarimetric measurements on rabbits' eyes indicate relative agreement between the modeled and experimental values of corneal birefringence. In addition, the observed rotation in the plane of polarized light for multiple wavelengths demonstrates the potential for using a dual-wavelength polarimetric approach to overcome the noise due to timevarying corneal birefringence. These results will ultimately aid us in the development of an appropriate eye coupling mechanism for in vivo polarimetric glucose measurements.

  20. Development of a microfabricated artificial limbus with micropockets for cell delivery to the cornea

    International Nuclear Information System (INIS)

    The aim of this study was to develop a synthetic alternative to the human corneal limbus for use initially as an ex vivo model in which to study corneal stem cell function within a niche environment and ultimately to develop an implantable limbus for future clinical use. Microstereolithography was used for the fabrication of polyethylene glycol diacrylate (PEGDA) based rings on a macroscopic (1.2 cm) scale containing unique microfeatures (pockets) which were then modified with fibronectin to promote cell adhesion. These rings were designed to mimic the limbal area of the eye containing structures of the approximate size and shape of the stem cell microenvironments found in the palisades of Vogt. The attachment of rabbit limbal fibroblasts and rabbit limbal epithelial cells to the PEGDA rings was increased by pretreating the microfabricated structures with biotinylated fibronectin. Cell outgrowth from fibronectin coated microfabricated structures was 50% greater than from rings without structures or fibronectin coating. The cell loaded rings were then placed on an ex vivo wounded cornea model and the outgrowth of cells to form a multilayered epithelium was observed. We suggest this is a new approach to investigating limbal stem cells niches and the first steps towards a new approach for corneal regeneration. (paper)

  1. The use of glycerol-preserved corneas in the developing world

    Directory of Open Access Journals (Sweden)

    Feilmeier Michael

    2010-01-01

    Full Text Available Corneal opacity is the third leading cause of blindness in the developing world and encompasses a wide variety of infectious, inflammatory and degenerative eye diseases. Most caes of corneal blindness are treatable with partial or full-thickness keratoplasty, provided adequate corneal tissue and surgical skill is available. However, access to sight-restoring keratoplasty in developing countries is limited by the lack of developed eye banking networks and a critical shortage of tissue suitable for transplantation. Beyond the developed world, corneal transplantation using fresh corneal tissue (FCT is further hindered by unreliable storage and transportation facilities, unorganized distribution networks, the cost-prohibitive nature of imported tissue, unreliable compliance with medications and follow-up instructions and inadequate health and education services. Glycerol-preserved corneas overcome many of these limitations inherent to the use of FCT. As surgical innovation in lamellar corneal surgery expands the potential use of acellular corneal tissue, long-term preservation techniques are being revisited as a way to increase availability of corneal tissue to corneal surgeons throughout the developing world. Herein, we discuss the advantages of using and the applications for glycerol-preserved corneal tissue throughout the developing world.

  2. A necessidade da imunofluorescência direta no diagnóstico da dermatose bolhosa por IgA The need for direct immunofluorescence in the diagnosis of IgA bullous dermatosis

    Directory of Open Access Journals (Sweden)

    Daniel Chang

    2012-02-01

    Full Text Available A dermatose bolhosa por imunoglobulina da classe A linear (DbIgA do adulto é uma doença autoimune rara caracterizada por formação de bolhas subepidérmicas e depósito linear de imunoglobulina da classe A (IgA na zona da membrana basal (ZMB. Por possuir aspectos clínicos e histológicos semelhantes a outras dermatoses bolhosas, principalmente a dermatite herpetiforme e o penfigoide bolhoso, faz-se necessária a realização de imunofluorescência direta para confirmação diagnóstica. Apresenta-se então, neste artigo, relato de caso ilustrando essa necessidade.Linear immunoglobulin A bullous dermatosis (DbIgA of adults is a rare autoimmune disease characterized by subepidermal blistering and linear deposits of immunoglobulin A (IgA in the basement membrane zone (BMZ. Owing to the fact it presents clinical and histological aspects similar to other bullous dermatosis, mainly dermatitis herpetiformis and bullous pemphigoid, direct immunofluorescence is required to confirm diagnosis. In this article, we describe a case that illustrates this need.

  3. A short-term study of corneal collagen cross-linking with hypo-osmolar riboflavin solution in keratoconic corneas

    Institute of Scientific and Technical Information of China (English)

    Shao-Feng; Gu; Zhao-Shan; Fan; Li-Hua; Wang; Xiang-Chen; Tao; Yong; Zhang; Chun-Qin; Wang; Ya; Wang; Guo-Ying; Mu

    2015-01-01

    AIM: To report the 3mo outcomes of collagen crosslinking(CXL) with a hypo-osmolar riboflavin in thin corneas with the thinnest thickness less than 400 μm without epithelium.METHODS: Eight eyes in 6 patients with age 26.2±4.8y were included in the study. All patients underwent CXL using a hypo-osmolar riboflavin solution after its de-epithelization. Best corrected visual acuity, manifest refraction, the thinnest corneal thickness, and endothelial cell density were evaluated before and 3mo after the procedure.RESULTS: The mean thinnest thickness of the cornea was 408.5 ±29.0 μm before treatment and reduced to369.8 ±24.8 μm after the removal of epithelium. With the application of the hypo-osmolar riboflavin solution, the thickness increased to 445.0 ±26.5 μm before CXL and recover to 412.5 ±22.7 μm at 3mo after treatment, P =0.659). Before surgery, the mean K-value of the apex of the keratoconus corneas was 57.6 ±4.0 diopters, and slightly decreased(54.7±4.9 diopters) after surgery(P =0.085). Mean best-corrected visual acuity was 0.55 ±0.23 logarithm of the minimal angle of resolution, and increased to 0.53±0.26 logarithm after surgery(P =0.879).The endothelial cell density was 2706.4 ±201.6 cells/mm2 before treatment, and slightly decreased( 2641. 2 ±218.2 cells/mm2) at last fellow up(P =0.002).CONCLUSION: Corneal collagen cross-linking with a hypo-osmolar riboflavin in thin corneas seems to be a promising treatment. Further study should be done to evaluate the safety and efficiency of CXL in thin corneas for the long-term.

  4. Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

    Directory of Open Access Journals (Sweden)

    van Beek Nina

    2012-08-01

    Full Text Available Abstract Background Various antigen-specific immunoassays are available for the serological diagnosis of autoimmune bullous diseases. However, a spectrum of different tissue-based and monovalent antigen-specific assays is required to establish the diagnosis. BIOCHIP mosaics consisting of different antigen substrates allow polyvalent immunofluorescence (IF tests and provide antibody profiles in a single incubation. Methods Slides for indirect IF were prepared, containing BIOCHIPS with the following test substrates in each reaction field: monkey esophagus, primate salt-split skin, antigen dots of tetrameric BP180-NC16A as well as desmoglein 1-, desmoglein 3-, and BP230gC-expressing human HEK293 cells. This BIOCHIP mosaic was probed using a large panel of sera from patients with pemphigus vulgaris (PV, n = 65, pemphigus foliaceus (PF, n = 50, bullous pemphigoid (BP, n = 42, and non-inflammatory skin diseases (n = 97 as well as from healthy blood donors (n = 100. Furthermore, to evaluate the usability in routine diagnostics, 454 consecutive sera from patients with suspected immunobullous disorders were prospectively analyzed in parallel using a the IF BIOCHIP mosaic and b a panel of single antibody assays as commonly used by specialized centers. Results Using the BIOCHIP mosaic, sensitivities of the desmoglein 1-, desmoglein 3-, and NC16A-specific substrates were 90%, 98.5% and 100%, respectively. BP230 was recognized by 54% of the BP sera. Specificities ranged from 98.2% to 100% for all substrates. In the prospective study, a high agreement was found between the results obtained by the BIOCHIP mosaic and the single test panel for the diagnosis of BP, PV, PF, and sera without serum autoantibodies (Cohen’s κ between 0.88 and 0.97. Conclusions The BIOCHIP mosaic contains sensitive and specific substrates for the indirect IF diagnosis of BP, PF, and PV. Its diagnostic accuracy is comparable with the conventional multi

  5. The role of lymphocytes, granulocytes, mast cells and their related cytokines in lesional skin of linear IgA bullous dermatosis.

    Science.gov (United States)

    Caproni, M; Rolfo, S; Bernacchi, E; Bianchi, B; Brazzini, B; Fabbri, P

    1999-06-01

    Linear IgA bullous dermatosis (LAD) is an acquired, heterogeneous, subepidermal blistering disease characterized by linear IgA deposits at the dermoepidermal basement membrane zone (BMZ), often with circulating IgA antibodies to the BMZ. The pathogenetic mechanism, possibly related to the immunophenotype of infiltrating cells, as well as the potential role of cytokines in determining bullous lesions, have not yet been elucidated. An immunohistochemical study was performed with a large panel of monoclonal antibodies [to CD3, CD4, CD8, CD25, CD1a, CD30, CD54, CD50, endothelial leucocyte adhesion molecule-1, vascular cell adhesion molecule-1, myeloperoxidase (MPO), eosinophil cationic protein EG1 and EG2, tryptase, HLA-DR, human interleukin (IL)-3, human IL-5, human IL-8, human IL-4, tumour necrosis factor (TNF)-alpha, interferon (IFN)-gamma and granulocyte/macrophage colony-stimulating factor] using the alkaline phosphatase-antialkaline phosphatase procedure on lesional and perilesional skin of nine patients (one male, eight female; age range 8 months-80 years) with clinical, histological and immunofluorescent proven LAD. The predominant infiltrating cells, distributed mostly inside and below the bullae, were neutrophils and eosinophils which showed intense activation (MPO +, EG1 +, EG2 +). The lymphocytic infiltrate, consisting principally of CD4 +, HLA-DR + and CD30 + T cells, had a predominantly perivascular distribution. Proinflammatory cytokines, such as TNF-alpha and IFN-gamma, showed a moderate focal expression on the dermal perivascular sites; IL-8 was found to have a particularly intense staining on all the epidermal cell layers and at perivascular and vascular sites. Other cytokines, such as IL-4 and IL-5, showed a prevalent intracytoplasmic staining on some cells of the dermal infiltrate (probably mastocytes and lymphocytes), and at the dermal-epidermal separation sites there was also an intense scattered distribution of IL-5. The specific tissue lesions

  6. Visual pigments, oil droplets, lens, and cornea characterization in the whooping crane (Grus americana)

    Science.gov (United States)

    Porter, Megan L.; Kingston, Alexandra C. N.; McCready, Robert; Cameron, Evan G.; Hofmann, Christopher M.; Suarez, Lauren; Olsen, Glenn H.; Cronin, Thomas W.; Robinson, Phyllis R.

    2014-01-01

    Vision has been investigated in many species of birds, but few studies have considered the visual systems of large birds and the particular implications of large eyes and long-life spans on visual system capabilities. To address these issues we investigated the visual system of the whooping crane, Grus americana (Gruiformes: Gruidae). G. americana (an endangered species) is one of only two North American crane species and represents a large, long-lived bird where ultraviolet sensitivity may be degraded by chromatic aberrations and entrance of ultraviolet light into the eye could be detrimental to retinal tissues. To investigate the whooping crane visual system we used microspectrophotometry to determine the absorbance spectra of retinal oil droplets and to investigate if the ocular media (i.e., the lens and cornea) absorbs UV light. In vitro expression and reconstitution was used to determine the absorbance spectra of rod and cone visual pigments. The rod visual pigments had wavelengths of peak absorbance (λmax) at 500 nm, while the cone visual pigments λmax values were determined to be 404 nm (SWS1), 450 nm (SWS2), 499 nm (RH2), and 561 nm (LWS), similar to other characterized bird visual pigment absorbance values. The oil droplet cutoff wavelength (λcut) values similarly fell within ranges recorded from other avian species: 576 nm (R-type), 522 nm (Y-type), 506 nm (P-type), and 448 nm (C-type). We confirm that G. americana has a violet-sensitive visual system, although based on the λmax of the SWS1 visual pigment (404 nm) may also have some ability for UV sensitivity.

  7. Mineralocorticoid hormone signaling regulates the 'epithelial sodium channel' in fibroblasts from human cornea.

    Science.gov (United States)

    Mirshahi, M; Mirshahi, S; Golestaneh, N; Nicolas, C; Mishal, Z; Lounes, K C; Hecquet, C; Dagonet, F; Pouliquen, Y; Agarwal, M K

    2001-01-01

    We investigated the regulation of sodium absorption by steroid hormones in embryologically diverse cells from the human eye. A cell extract from human corneal fibroblasts was positive for both the epithelial sodium channel (ENaC) and the mineralocorticoid receptor (MCR) as 82- to 85-kD and 102-kD bands, respectively, by the Western blot technique. In fluorescent, confocal and electron microscopy, the MCR was revealed as a nucleocytoplasmic protein, whereas the ENaC was almost exclusively membrane bound; both appeared aligned along actin filaments of corneal keratocytes, and both were widely colocalized in various cell types of human cornea in situ. Following reverse transcription and amplification of total RNA isolated from corneal fibroblasts, the ENaC and MCR genes in the PCR product were evident as predicted bands of 520 and 843 bp, respectively, whose sequence exhibited 100% identity with those from known human sources. The multiplication of corneal fibroblasts was influenced by both the MCR-specific antagonist RU 26752 and the natural hormone aldosterone, and these steroids also stimulated protein phosphorylation. In quantitative PCR, both the basal and aldosterone-induced levels of ENaC were diminished by the MCR-specific antagonist ZK 91587. Consequently, the ocular sodium channel appears to be regulated by steroid signalling in cells of diverse embryological origins, contrary to the existing notions where (a) this process would be limited exclusively to the epithelial cells and (b) ocular sodium transport would be regulated via the Na(+)-K(+)-ATPase in the basolateral membrane. PMID:11114599

  8. Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea

    Directory of Open Access Journals (Sweden)

    O'Brien Terrence P

    2003-10-01

    Full Text Available Abstract Background Matrix metalloproteinases play an important role in extracellular matrix deposition and degradation. Based on previous clinical observations of corneal perforations during topical fluoroquinolone treatment, we decided to evaluate the comparative effects of various fluoroquinolone eye drops on the expression of matrix metalloproteinases (MMPs in cornea. Methods Eighty female Lewis rats were divided into two experimental groups: intact and wounded corneal epithelium. Uniform corneal epithelial defects were created in the right eye with application of 75% alcohol in the center of the tissue for 6 seconds. The treatment groups were tested as follows: 1 Tear drops: carboxymethylcellulose sodium 0.5 % (Refresh, Allergan; 2 Ciprofloxacin 0.3% (Ciloxan, Alcon; 3 Ofloxacin 0.3%(Ocuflox, Allergan; 4 Levofloxacin 0.5%(Quixin, Santen. Eye drops were administered 6 times a day for 48 hours. Rats were sacrificed at 48 hours. Immunohistochemical analysis and zymography were conducted using antibodies specific to MMPs-1, 2, 8 and 9. Results MMP-1, MMP-2, MMP-8 and MMP-9 expression were detected at 48 hrs in undebrided corneal epithelium groups treated with the topical fluoroquinolones. No statistical difference was observed in quantitative expression of MMPs among ciprofloxacin 0.3%, ofloxacin 0.3%, levofloxacin 0.5%. When the artificial tear group and the fluoroquinolone groups with corneal epithelial defect were compared, increased expression of MMPs was observed as a result of the wound healing process. However, the fluoroquinolone treated group exhibited high statistically significantly levels of MMPs expression. Conclusions Our study provides preliminary evidence that topical application of fluoroquinolone drugs can induce the expression of MMP-1, MMP-2, MMP-8 and MMP-9 in the undebrided corneal epithelium compared to artificial tear eye drops.

  9. Effect of biaxial versus coaxial microincision cataract surgery on optical quality of the cornea

    Directory of Open Access Journals (Sweden)

    Tamer Fahmy Eliwa

    2015-01-01

    Full Text Available Context: Visual function is determined by a combination of the cornea, which has a larger effect and internal aberrations generated by the intraocular lens and those induced by the surgery. These corneal refractive changes are related to the location and size of the corneal incision. The smaller the incision, the lower the aberrations and the better the optical quality. Aims: To compare the effect of uneventful coaxial versus biaxial microincision cataract surgery (MICS on the corneal aberrations. Settings and Design: Retrospective interventional nonrandomized comparative case study comprised 40 eyes of 36 patients with primary senile cataract. Subjects and Methods: They were divided into two groups: Group I (20 eyes had operated by biaxial MICS and Group II (20 eyes had operated by coaxial MICS. Each group were assessed by corneal topography and wavefront analysis over 6 mm pupil size preoperatively and 1-month postoperatively. Statistical Analysis Used: Statistical analysis was performed using SPSS for Windows (version 17.0.1, SPSS, Inc.. The paired t-test was used to compare the mean values of corneal aberrations preoperatively and 1-month postoperatively in each group. Results: There was a significant increase in trefoil and quatrefoil in biaxial MICS (P = 0.063, 0.032 respectively while other aberrations insignificantly changed. The coaxial MICS showed a significant increase in root mean square (RMS of total high order aberrations (HOAs (P = 0.02 and coma (0.028, but not the others. In comparison to each other, there was the insignificant difference as regards astigmatism, RMS of individual and total HOAs. Conclusions: Coaxial and biaxial MICS are neutral on corneal astigmatism and aberrations.

  10. Mutations in the 180-kD bullous pemphigoid antigen (BPAG2), a hemidesmosomal transmembrane collagen (COL17A1), in generalized atrophic benign epidermolysis bullosa.

    Science.gov (United States)

    McGrath, J A; Gatalica, B; Christiano, A M; Li, K; Owaribe, K; McMillan, J R; Eady, R A; Uitto, J

    1995-09-01

    Junctional epidermolysis bullosa (JEB) is a heterogeneous autosomal recessively inherited blistering skin disorder associated with fragility at the dermal-epidermal junction. Characteristic ultrastructural findings in JEB are abnormalities in the hemidesmosome-anchoring filament complexes. These focal attachment structures, which extend from the intracellular compartment of the basal keratinocytes to the underlying basement membrane, have been shown to be hypoplastic or rudimentary in different forms of JEB. Previously, in different JEB phenotypes, mutations have been found in the three genes for the anchoring filament component laminin 5 (LAMA3, LAMB3, and LAMC2) and in the gene for the hemidesmosome-associated integrin beta 4 subunit. Here, we describe the first mutations in the gene encoding the 180-kD bullous pemphigoid antigen (BPAG2), a transmembranous hemidesmosomal collagen, also known as type XVII collagen (COL17A1). The patient is affected with generalized atrophic benign epidermolysis bullosa (GABEB), a rare variant of JEB, and is a compound heterozygote for premature termination codons on both alleles. These novel findings emphasize the molecular heterogeneity of this group of genodermatoses, and attest to the importance of BPAG2 in maintaining adhesion between the epidermis and the dermis. PMID:7550320

  11. Linear IgA Bullous Dermatosis in a Patient with Graves' Disease%线状IgA大疱性皮病合并Graves病1例

    Institute of Scientific and Technical Information of China (English)

    黄翀; 陈连军

    2011-01-01

    患者女,28岁.背部反复出现水疱,伴瘙痒5个月,加重并泛发至全身4天,病程中同时出现甲状腺功能亢进.皮损组织病理和直接免疫荧光检查均提示线状IgA大疱性皮病.皮肤病和甲状腺疾病分别经糖皮质激素和同位素治疗得到缓解.%A 28-year-old female had suffered from pruritic vesicles on the back for 5 months, which rapidly progressed to the whole body within 4 days before admission to our hospital. The skin disease was accompanied by hy-perthymidism. Skin biopsy and direct imrnunofluoreseenee proved linear IgA bullous dermatosis. The skin disease and thyroid desease achieved remission with corticosteroid and 131I treatment respectively.

  12. Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily

    Energy Technology Data Exchange (ETDEWEB)

    Jefferson,J.; Ciatto, C.; Shapiro, l.; Liem, R.

    2007-01-01

    Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 {angstrom} resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.

  13. A comparison of glycosaminoglycan distributions, keratan sulphate sulphation patterns and collagen fibril architecture from central to peripheral regions of the bovine cornea.

    Science.gov (United States)

    Ho, Leona T Y; Harris, Anthony M; Tanioka, Hidetoshi; Yagi, Naoto; Kinoshita, Shigeru; Caterson, Bruce; Quantock, Andrew J; Young, Robert D; Meek, Keith M

    2014-09-01

    This study investigated changes in collagen fibril architecture and the sulphation status of keratan sulphate (KS) glycosaminoglycan (GAG) epitopes from central to peripheral corneal regions. Freshly excised adult bovine corneal tissue was examined as a function of radial position from the centre of the cornea outwards. Corneal thickness, tissue hydration, hydroxyproline content, and the total amount of sulphated GAG were all measured. High and low-sulphated epitopes of keratan sulphate were studied by immunohistochemistry and quantified by ELISA. Chondroitin sulphate (CS) and dermatan sulphate (DS) distributions were observed by immunohistochemistry following specific enzyme digestions. Electron microscopy and X-ray fibre diffraction were used to ascertain collagen fibril architecture. The bovine cornea was 1021±5.42 μm thick at its outer periphery, defined as 9-12 mm from the corneal centre, compared to 844±8.10 μm at the centre. The outer periphery of the cornea was marginally, but not significantly, more hydrated than the centre (H=4.3 vs. H=3.7), and was more abundant in hydroxyproline (0.12 vs. 0.06 mg/mg dry weight of cornea). DMMB assays indicated no change in the total amount of sulphated GAG across the cornea. Immunohistochemistry revealed the presence of both high- and low-sulphated epitopes of KS, as well as DS, throughout the cornea, and CS only in the peripheral cornea before the limbus. Quantification by ELISA, disclosed that although both high- and low-sulphated KS remained constant throughout stromal depth at different radial positions, high-sulphated epitopes remained constant from the corneal centre to outer-periphery, whereas low-sulphated epitopes increased significantly. Both small angle X-ray diffraction and TEM analysis revealed that collagen fibril diameter remained relatively constant until the outer periphery was reached, after which fibrils became more widely spaced (from small angle x-ray diffraction analysis) and of larger diameter

  14. T-style keratoprosthesis based on surface-modified poly (2-hydroxyethyl methacrylate) hydrogel for cornea repairs

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Jun [Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); Sun, Jianguo [Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); State Key Laboratory of Molecular Engineering of Polymers, Fudan University (China); Hong, Jiaxu [Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); Wang, Wentao [Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); Wei, Anji [Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); Le, Qihua [Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China); Xu, Jianjiang, E-mail: jianjiang-xu@163.com [Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University (China); Key Laboratory of Myopia, Ministry of Health, Fudan University (China); Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University (China)

    2015-05-01

    Corneal disease is a common cause of blindness, and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents a novel T-style design of a keratoprosthesis and its preparation methods, in which a mechanically and structurally effective artificial cornea is made based on a poly(2-hydroxyethyl methacrylate) hydrogel. The porous skirt was modified with hyaluronic acid and cationized gelatin, and the bottom of the optical column was coated with poly(ethylene glycol). The physical properties of the T-style Kpro were analyzed using ultraviolet and visible spectrophotometry and electron scanning microscopy. The surface chemical properties were characterized using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The surface modification in the spongy skirt promoted cell adhesion and produced a firm bond between the corneal tissue and the implant device, while the surface modification in the optic column resisted cell adhesion and prevented retroprosthetic membrane formation. Through improved surgical techniques, the novel T-style keratoprosthesis provides enough mechanical stability to facilitate long-term biointegration with the host environment. In vivo implantation experiments showed that the T-style keratoprosthesis is a promising cornea alternative for patients with severe limbal stem cell deficiency and corneal opacity. - Highlights: • T-style keratoprosthesis was designed and prepared based on a PHEMA hydrogel. • Selective surface modifications effectively regulated cells' selective adhesion. • T-style keratoprosthesis provides enough mechanical stability to facilitate long-term biointegration with host tissues.

  15. T-style keratoprosthesis based on surface-modified poly (2-hydroxyethyl methacrylate) hydrogel for cornea repairs

    International Nuclear Information System (INIS)

    Corneal disease is a common cause of blindness, and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents a novel T-style design of a keratoprosthesis and its preparation methods, in which a mechanically and structurally effective artificial cornea is made based on a poly(2-hydroxyethyl methacrylate) hydrogel. The porous skirt was modified with hyaluronic acid and cationized gelatin, and the bottom of the optical column was coated with poly(ethylene glycol). The physical properties of the T-style Kpro were analyzed using ultraviolet and visible spectrophotometry and electron scanning microscopy. The surface chemical properties were characterized using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The surface modification in the spongy skirt promoted cell adhesion and produced a firm bond between the corneal tissue and the implant device, while the surface modification in the optic column resisted cell adhesion and prevented retroprosthetic membrane formation. Through improved surgical techniques, the novel T-style keratoprosthesis provides enough mechanical stability to facilitate long-term biointegration with the host environment. In vivo implantation experiments showed that the T-style keratoprosthesis is a promising cornea alternative for patients with severe limbal stem cell deficiency and corneal opacity. - Highlights: • T-style keratoprosthesis was designed and prepared based on a PHEMA hydrogel. • Selective surface modifications effectively regulated cells' selective adhesion. • T-style keratoprosthesis provides enough mechanical stability to facilitate long-term biointegration with host tissues

  16. Role of mesenchymal stem cells on cornea wound healing induced by acute alkali burn.

    Directory of Open Access Journals (Sweden)

    Lin Yao

    Full Text Available The aim of this study was to investigate the effects of subconjunctivally administered mesenchymal stem cells (MSCs on corneal wound healing in the acute stage of an alkali burn. A corneal alkali burn model was generated by placing a piece of 3-mm diameter filter paper soaked in NaOH on the right eye of 48 Sprague-Dawley female rats. 24 rats were administered a subconjunctival injection of a suspension of 2×10(6 MSCs in 0.1 ml phosphate-buffered saline (PBS on day 0 and day 3 after the corneal alkali burn. The other 24 rats were administered a subconjunctival injection of an equal amount of PBS as a control. Deficiencies of the corneal epithelium and the area of corneal neovascularization (CNV were evaluated on days 3 and 7 after the corneal alkali burn. Infiltrated CD68(+ cells were detected by immunofluorescence staining. The mRNA expression levels of macrophage inflammatory protein-1 alpha (MIP-1α, tumor necrosis factor-alpha (TNF-α, monocyte chemotactic protein-1 (MCP-1 and vascular endothelial growth factor (VEGF were analyzed using real-time polymerase chain reaction (real-time PCR. In addition, VEGF protein levels were analyzed using an enzyme-linked immunosorbent assay (ELISA. MSCs significantly enhanced the recovery of the corneal epithelium and decreased the CNV area compared with the control group. On day 7, the quantity of infiltrated CD68(+ cells was significantly lower in the MSC group and the mRNA levels of MIP-1α, TNF-α, and VEGF and the protein levels of VEGF were also down-regulated. However, the expression of MCP-1 was not different between the two groups. Our results suggest that subconjunctival injection of MSCs significantly accelerates corneal wound healing, attenuates inflammation and reduces CNV in alkaline-burned corneas; these effects were found to be related to a reduction of infiltrated CD68(+ cells and the down-regulation of MIP-1α, TNF-α and VEGF.

  17. Systematic infrared ablations with the mark III FEL at 2.94 μm in the chicken cornea

    International Nuclear Information System (INIS)

    Previous studies with the free electron laser have only assessed the corneal tissue response in ex vivo models. This is the first free electron laser investigation that has examined the clinical and histologic response of the cornea in vivo. Methods. The Mark III free electron laser was tuned to 2.94 μm for a total of 212 separate in vivo 1 mm spot ablations at varied fluences (0.4-2.04 J/cm2), repetition rates (1-15 Hz), and number of pulses (1-32). Also, 22 separate in vivo central scanning patterns were performed. Results. For the in vivo chicken cornea, the threshold of ablation is approximately 0.64 J/cm2 at a repetition rate of 10 Hz. Clinical biomicroscopy and light microscopy revealed parameter-dependent collateral damage (10-40) μm) induced by laser energy. Wound healing following free electron laser ablation exhibits patterns similar to those seen in excimer laser anterior keratectomy, including increased epithelial thickness, new collagen deposition, increased numbers of keratocytes, and patterns congruent with attempted profiles of myopic ablation. Conclusions. With further study, continued parameter adjustment, and refined delivery schemes, the potential for marked improvement and possible application appears promising

  18. Gaussian process prediction of the stress-free configuration of pre-deformed soft tissues: Application to the human cornea.

    Science.gov (United States)

    Businaro, Elena; Studer, Harald; Pajic, Bojan; Büchler, Philippe

    2016-04-01

    Image-based modeling is a popular approach to perform patient-specific biomechanical simulations. One constraint of this technique is that the shape of soft tissues acquired in-vivo is deformed by the physiological loads. Accurate simulations require determining the existing stress in the tissues or their stress-free configurations. This process is time consuming, which is a limitation to the dissemination of numerical planning solutions to clinical practice. In this study, we propose a method to determine the stress-free configuration of soft tissues using a Gaussian process (GP) regression. The prediction relies on a database of pre-calculated results to enable real time predictions. The application of this technique to the human cornea showed a level of accuracy five to ten times higher than the accuracy of the topographic device used to obtain the patients' anatomy; results showed that for almost all optical indices, the predicted curvature error did not exceed 0.025D, while the wavefront aberration percentage error did not overcome 5%. In this context, we believe that GP models are suitable for predicting the stress free configuration of the cornea and can be used in planning tools based on patient-specific finite element simulations. Due to the high level of accuracy required in ophthalmology, this approach is likely to be appropriate for other applications requiring the definition of the relaxed shape of soft tissues. PMID:26920075

  19. Behaviour of glutathione in the cornea epithelium of cattle and rabbits under the influence of infrared radiation

    International Nuclear Information System (INIS)

    After creating a keratitis photoelectrica by means of a mercury/quartz analysis lamp, the concentration of GSH (glutathion, reduced) and GSSG (glutathion, oxidised) in the cornea epithelium of cattle and of rabbits at different times after irradiation was determined. The question of whether ultraviolet radiation considerably influences the GSH/GSSG quotient before the keratitis photoelectrica is fully formed morphologically, could be answered as follows: There is no considerable change in the GSH/GSSG quotient during the first 7-10 hours after the irradiation. Then, when there is, also morphologically, a keratitis photoelectrica, the GSH/GSSG quotient is doubled. The linkage of the GSH/GSSG system and hexosephosphatshunt via the NADP/NADPH system leads us to the conclusion that irradiation does not cause any direct changes in the GSH/GSSG system. Only when the cornea epithelium visably disintegrates an increased reduction of the glutathion occurs, like in several other damaging influences. Thus the found increase of the GSH/GSSG quotient 7-10 hours after ultraviolet irradiation might be unspecific. (orig./AJ)

  20. Analysis of two precipitation methods on the yield, structural features and activity of sulfated polysaccharides from Gracilaria cornea (Rhodophyta

    Directory of Open Access Journals (Sweden)

    Ricardo Basto Souza

    2015-03-01

    Full Text Available The global demand for natural products from seaweeds has increased worldwide; however, no description of the use of isoamly alcohol (IAA for obtaining of sulfated polysaccharides (SPs has been reported. We investigated the efficiency of two precipitation methods (M in obtaining SPs from the red seaweed Gracilaria cornea. SPs enzymatically isolated were concentrated with cetylpyridinium chloride (M I or IAA (M II and extracts were examined with regard to their yield, structural features and in vitro effects on the activated partial thromboplastin time (APTT using normal human plasma and standard heparin (193 IU mg-1. Yield difference reached 12.99%. Quantitative determination of sulfate was similar between the two methods (̴ 26%, but extracts revealed different pattern on charge density by agarose gel electrophoresis. Whereas both extracts revealed as agarocolloids, alternative M II was also efficient for lipids, proteins and nucleic acids according to the infrared analysis. Extracts had virtually no effect on APPT (1.95 and 2 IU mg-1 for M I and M II, respectively. The results revealed IAA as an alternative solvent for obtaining SPs from the red seaweed G. cornea, depending on the industry’ usage criterion.

  1. Nature-Derived Aloe Vera Gel Blended Silk Fibroin Film Scaffolds for Cornea Endothelial Cell Regeneration and Transplantation.

    Science.gov (United States)

    Kim, Do Kyung; Sim, Bo Ra; Khang, Gilson

    2016-06-22

    The goal of this study was to fabricate an appropriate replacement for cadaveric corneas to overcome a shortage of cadaveric corneas for transplantation. In this study, we fabricated transparent ultrathin film scaffolds with nature-derived aloe vera (AV) gel and silk fibroin (SF) for corneal endothelial cells (CECs). The scaffolds were subjected to analysis of transparency and contact angle using field emission scanning electron microscopy (FESEM) and Fourier transform infrared spectroscopy to determine their physical and chemical properties. FESEM images revealed that the critical morphology of CECs was formed on the AV gel in the blend with SF rather than in the scaffold with SF alone. The cell proliferation, phenotype, and specific gene marker expressions for CECs were determined by MTT assays, immunofluorescence, and reverse transcription polymerase chain reactions. Incorporation of a small amount of AV gel increased the cell viability and maintained its functions well. The scaffolds were easily handled for transplantation into rabbit eyes with small incisions and examined by their transparency after transplantation and histological staining. The scaffolds attached to the surface of the corneal stroma and integrated with surrounding corneal tissue without a significant inflammatory reaction. These results indicate that AV blended SF film scaffolds might be a suitable substitute for alternative corneal grafts for transplantation. PMID:27243449

  2. Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope

    Science.gov (United States)

    Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten

    2016-03-01

    Five dimensional microscopy with a 12-fs laser scanning microscope based on spectrally resolved two-photon autofluorescence lifetime and second-harmonic generation (SHG) imaging was used to characterize all layers of the porcine cornea. This setup allowed the simultaneous excitation of both metabolic cofactors, NAD(P)H and flavins, and their discrimination based on their spectral emission properties and fluorescence decay characteristics. Furthermore, the architecture of the stromal collagen fibrils was assessed by SHG imaging in both forward and backward directions. Information on the metabolic state and the tissue architecture of the porcine cornea were obtained with subcellular resolution, and high temporal and spectral resolutions.

  3. 大疱性皮肤病53例免疫荧光和组织病理检查结果分析%Analysis of Immunofluorescence and Histopathologic Examination on 53 Cases of Bullous Diseases

    Institute of Scientific and Technical Information of China (English)

    张东红; 韦海明; 黄琦涛; 李玉秋

    2011-01-01

    目的 探讨直接免疫荧光(DIF)和盐裂皮肤DIF技术在诊断大疱性皮肤病中的价值.方法 采用DIF和盐裂皮肤DIF技术检测53例大疱性皮肤病标本,并与其组织病理检查结果进行比较.结果 DIF和盐裂皮肤DIF技术诊断该类疾病的阳性率(90.57%)显著高于对其进行组织病理检查的阳性率(73.58%),差异有统计学意义(P<0.05).结论 DIF和盐裂皮肤DIF技术仍是目前诊断和鉴别诊断大疱性皮肤病的可靠方法.%Objective To explore the diagnostic valuation of direct immunofluorescence and sodium chloride-separated skin direct immunofluorescence in bullous diseases. Methods Fifty-three cases of bullous diseases were detected by direct immunofluorescence and sodium chloride-separated skin direct immunofluorescence, and compared with histopalhologic examination. Results The diagnostic rate of DIF and sodium chloride-separated skin DIF was significantly higher than that of histopathologic examination (90. 57% vs 73. 58% ,P < 0.05). Conclusion At present, DIF and sodium chloride-separated skin DIF is still a more reliable technique for the diagnosis and differentiation of bullous diseases.

  4. Accuracy of indirect immunofluorescence on sodium chloride-split skin in the differential diagnosis of bullous pemphigoid and epidermolysis bullosa acquisita

    Directory of Open Access Journals (Sweden)

    Baoqi Yang

    2011-01-01

    Full Text Available Background: Previous reports have shown that indirect immunofluorescence (IIF performed on sodium chloride-split skin (SSS is helpful to differentiate epidermolysis bullosa acquisita (EBA from bullous pemphigoid (BP. Antibodies of BP may bind to the epidermal side of SSS, while antibodies of EBA bind to the dermal side. Aims: To determine the accuracy of IIF-SSS in the differential diagnosis of EBA and BP utilizing immunoblotting (IB analysis. Methods: Sera from 78 patients, diagnosed with BP by clinical features, histopathology, and direct immunofluorescence (DIF, were assayed using IIF-SSS and IB. Results: Of the 43 serum samples with an epidermal reaction to IIF-SSS assay, 42 were recognized with BP antigens (180 kDa or 230 kDa. Of the 11 serum samples with a dermal reaction pattern, 7 were recognized with the 290 kDa antigen of EBA and 3 with sera bound BP antigens. Seven serum samples with epidermal and dermal combined staining, of which 5 of them reacted with BP antigens, 1 reacted with both BP and EBA antigens. One serum sample from each group showed a negative result by IB. Approximately 9.0% (7/78 of patients diagnosed with BP using regular methods were actually EBA. Conclusions: Epidermal reaction using the IIF-SSS assay highly correlated with the diagnosis of BP. However, dermal reactions correlated poorly with EBA, with some serum samples from BP patients binding to dermal-side antigens. In both epidermal and dermal stained sera using IIF-SSS, there was a possibility of BP and EBA. Differential diagnosis should be confirmed using IB, especially in cases of dermal and double staining patterns assayed using IIF-SSS.

  5. IgG/IgE bullous pemphigoid with CD45 lymphocytic reactivity to dermal blood vessels, nerves and eccrine sweat glands

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu-Velez

    2010-01-01

    Full Text Available Context: Bullous pemphigoid (BP, the most common autoimmune blistering disease, is mediated by autoantibodies. BP primarily affects the elderly and is characterized by the development of urticarial plaques surmounted by subepidermal blisters, and the deposition of immunoglobulins and complement at the basement membrane zone (BMZ of the skin. BP is immunologically characterized by the development of autoantibodies targeting two structural proteins of the hemidesmosomes, BP180 (collagen XVII and BP230 (BPAG1. Case Report: A 63 -year-old Caucasian female patient was evaluated for a 4 day history of several itching, erythematous blisters on her extremities. Biopsies for hematoxylin and eosin (H&E examination, as well as Periodic acid-Schiff (PAS, immunohistochemistry (IHC and direct immunofluorescence (DIF analysis were performed. Results: H&E demonstrated a subepidermal blister, with partial re-epithelialization of the blister floor. Within the blister lumen, numerous neutrophils were present, with occasional eosinophils and lymphocytes also noted. Within the dermis, a mild, superficial, perivascular and periadnexal infiltrate of lymphocytes, histiocytes and occasional eosinophils was identified, with mild perivascular leukocytoclastic debris. The PAS stain was positive at the BMZ, and around selected blood vessels, nerves and sweat glands. DIF revealed linear deposits of IgG and Complement/C3 and fibrinogen at the BMZ, and around selected dermal nerves, blood vessels and sweat glands. Strong granular deposits of IgE were also observed, colocalizing with monoclonal antibodies to Collagen IV (CIV. By IHC, positive CD45 staining of lymphocytes was seen surrounding selected dermal blood vessels, eccrine sweat glands, and nerves. Conclusion : The patient displayed IgG, IgE, and fibrinogen autoantibodies against the BMZ, as well as around some dermal nerves and sweat glands; their binding in the skin could trigger complement activation. In addition, the

  6. Amniotic membrane immobilized poly(vinyl alcohol) hybrid polymer as an artificial cornea scaffold that supports a stratified and differentiated corneal epithelium.

    Science.gov (United States)

    Uchino, Yuichi; Shimmura, Shigeto; Miyashita, Hideyuki; Taguchi, Tetsushi; Kobayashi, Hisatoshi; Shimazaki, Jun; Tanaka, Junzo; Tsubota, Kazuo

    2007-04-01

    Poly(vinyl alcohol) (PVA) is a biocompatible, transparent hydrogel with physical strength that makes it promising as a material for an artificial cornea. In our previous study, type I collagen was immobilized onto PVA (PVA-COL) as a possible artificial cornea scaffold that can sustain a functional corneal epithelium. The cellular adhesiveness of PVA in vitro was improved by collagen immobilization; however, stable epithelialization was not achieved in vivo. To improve epithelialization in vivo, we created an amniotic membrane (AM)-immobilized polyvinyl alcohol hydrogel (PVA-AM) for use as an artificial cornea material. AM was attached to PVA-COL using a tissue adhesive consisting of collagen and citric acid derivative (CAD) as a crosslinker. Rabbit corneal epithelial cells were air-lift cultured with 3T3 feeder fibroblasts to form a stratified epithelial layer on PVA-AM. The rabbit corneal epithelial cells formed 3-5 layers of keratin-3-positive epithelium on PVA-AM. Occludin-positive cells were observed lining the superficial epithelium, the gap-junctional protein connexin43-positive cells was localized to the cell membrane of the basal epithelium, while both collagen IV were observed in the basement membrane. Epithelialization over implanted PVA-AM was complete within 2 weeks, with little inflammation or opacification of the hydrogel. Corneal epithelialization on PVA-AM in rabbit corneas improved over PVA-COL, suggesting the possibility of using PVA-AM as a biocompatible hybrid material for keratoprosthesis. PMID:16924609

  7. Comparison of the in vitro anticollagenase efficacy of homologous serum and plasma on degradation of corneas of cats, dogs, and horses.

    Science.gov (United States)

    Conway, Emily D; Stiles, Jean; Townsend, Wendy M; Weng, Hsin-Yi

    2016-06-01

    OBJECTIVE To compare the anticollagenase efficacy of fresh feline, canine, and equine serum and plasma on in vitro corneal degradation. SAMPLE Grossly normal corneas from recently euthanized dogs, cats, and horses and fresh serum and plasma from healthy dogs, cats, and horses. PROCEDURES Serum and plasma were pooled by species and used for in vitro experiments. Corneas were collected and stored at -80°C. Sections of cornea were dried, weighed, and incubated in saline (0.9% NaCl) solution with clostridial collagenase and homologous fresh serum or plasma. Corneal degradation was assessed as the percentage of corneal weight loss and hydroxyproline concentration, compared with results for positive and negative control samples. RESULTS Homologous fresh serum and plasma significantly reduced the percentage of corneal weight loss, compared with results for positive control samples. No significant difference was found in percentage of corneal weight loss between incubation with serum or plasma for feline, canine, and equine corneas. Canine serum and plasma significantly reduced hydroxyproline concentrations, whereas inclusion of feline and equine serum or plasma did not, compared with results for positive control samples. Hydroxyproline concentrations were moderately correlated with percentage of corneal weight loss for feline samples and weakly correlated for equine samples, but they were not correlated for canine samples. CONCLUSIONS AND CLINICAL RELEVANCE In this study, the anticollagenase efficacy of fresh feline, canine, and equine serum was not different from that of plasma. Plasma should be an acceptable substitute for serum in the topical treatment of keratomalacia. PMID:27227501

  8. Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea

    Czech Academy of Sciences Publication Activity Database

    Čejková, Jitka; Vejražka, M.; Pláteník, J.; Štípek, S.

    2004-01-01

    Roč. 39, - (2004), s. 1537-1543. ISSN 0531-5565 R&D Projects: GA ČR GA304/03/0419 Institutional research plan: CEZ:AV0Z5039906; CEZ:AV0Z5008914 Keywords : aging * cornea Subject RIV: FF - HEENT, Dentistry Impact factor: 2.880, year: 2004

  9. The oxidant/antioxidant imbalance appearing in the cornea after UVB rays is more pronounced in eyes where microbes were isolated

    Czech Academy of Sciences Publication Activity Database

    Čejková, Jitka

    New Mexico : CORNEA, 2000, s. 109. [International Congress of Eye Research /14./. New Mexico (US), 15.10.2000-20.10.2000] R&D Projects: GA ČR GV307/96/K226; GA MZd NG16; GA ČR GA304/00/1635 Institutional research plan: CEZ:AV0Z5039906 Subject RIV: FF - HEENT, Dentistry

  10. Application of Multivariete Analysis on High-Resolution Magic Angle Spinning (HR-MAS) 1H NMR Spectra of Rabbit Cornea

    Czech Academy of Sciences Publication Activity Database

    Tessem, M. B.; Bathen, T. F.; Čejková, Jitka; Midelfart, A.

    Basel: Karger, 2004. s. 67. ISSN 0030-3747. [EVER 2004 (European Association for Vision and Eye Research). 24.09.2004-27.09.2004, Vilamoura] R&D Projects: GA ČR GA304/03/0419 Keywords : rabbit cornea Subject RIV: FF - HEENT, Dentistry

  11. Enhanced Wound Healing, Kinase and Stem Cell Marker Expression in Diabetic Organ-Cultured Human Corneas Upon MMP-10 and Cathepsin F Gene Silencing

    OpenAIRE

    Saghizadeh, Mehrnoosh; Epifantseva, Irina; Hemmati, David M.; Ghiam, Chantelle A.; Brunken, William J; Ljubimov, Alexander V

    2013-01-01

    Adenovirus-driven shRNA silencing of select proteinases upregulated in diabetic corneas restored normal wound healing time, the expression of diabetes-altered markers including limbal stem cell markers, and patterns of activated EGFR and Akt in human diabetic corneal organ cultures. The maximum effect was obtained combining proteinase shRNA with c-met overexpression.

  12. A Raman scattering and FT-IR spectroscopic study on the effect of the solar radiation in Antarctica on bovine cornea

    Science.gov (United States)

    Yamamoto, Tatsuyuki; Murakami, Naoki; Yoshikiyo, Keisuke; Takahashi, Tetsuya; Yamamoto, Naoyuki

    2010-01-01

    The Raman scattering and FT-IR spectra of the corneas, transported to the Syowa station in Antarctica and exposed to the solar radiation of the mid-summer for four weeks, were studied to reveal that type IV collagen involved in corneas were fragmented. The amide I and III Raman bands were observed at 1660 and 1245 cm -1, respectively, and the amide I and II infrared bands were observed at 1655 and 1545 cm -1, respectively, for original corneas before exposure. The background of Raman signals prominently increased and the ratio of amide II infrared band versus amide I decreased by the solar radiation in Antarctica. The control experiment using an artificial UV lamp was also performed in laboratory. The decline rate of the amide II/amide I was utilized for estimating the degree of fragmentation of collagen, to reveal that the addition of vitamin C suppressed the reaction while the addition of sugars promoted it. The effect of the solar radiation in Antarctica on the corneas was estimated as the same as the artificial UV lamp of four weeks (Raman) or one week (FT-IR) exposure.

  13. Irradiation of the rabbit cornea with UVB rays stimulates the expression of nitric oxide synthases-generated nitric oxide and the formation of cytotoxic nitrogen-related oxidants

    Czech Academy of Sciences Publication Activity Database

    Čejková, Jitka; Ardan, Taras; Andonova, Janetta; Zídek, Zdeněk

    2005-01-01

    Roč. 20, - (2005), s. 467-473. ISSN 0213-3911 R&D Projects: GA ČR GA304/03/0419 Institutional research plan: CEZ:AV0Z5008914 Keywords : UVB rays * Reactive nitrogen species * Cornea Subject RIV: FF - HEENT, Dentistry Impact factor: 2.023, year: 2005

  14. Scattered radiation dose to radiologist's cornea, thyroid and gonads while performing some x-ray fluoroscopic investigations

    International Nuclear Information System (INIS)

    The mankind has been immensely benefited from discovery of X-ray and it has found wide spread application in diagnosis and treatment. Radiation is harmful and can produce somatic and genetic effects in the exposed person. International Commission on Radiation Protection (ICRP) has recommended a system of dose limitation based on principle of ALARA. All the efforts should be made to keep the radiation dose to the radiation worker as low as possible. Fluoroscopy gives maximum dose to the patient and staff and hence we have attempted to quantify the scattered radiation dose to the cornea, thyroid and gonads of the radiologist performing fluoroscopic examinations such as barium meal, barium swallow, barium enema, myelography, histerosalpingography and fracture reduction. Thermoluminescence dosimetry (TLD) method using CaSO4:Dy TLD disc was employed for these measurements. Use of lead apron has reduced the dose to radiologist's gonad. (author). 3 refs., 4 tabs

  15. Poly (ε-caprolactone nanofibrous ring surrounding a polyvinyl alcohol hydrogel for the development of a biocompatible two-part artificial cornea

    Directory of Open Access Journals (Sweden)

    Bakhshandeh H

    2011-07-01

    Full Text Available Haleh Bakhshandeh1, Masoud Soleimani2, Saied Shah Hosseini3, Hassan Hashemi3, Iman Shabani4, Abbas Shafiee5, Amir Houshang Behesht Nejad6, Mohammad Erfan1, Rassoul Dinarvand7, Fatemeh Atyabi71Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti Medical University, Tehran, Iran; 2Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran; 3Noor Ophthalmology Research Center, Noor Eye Hospital, Tehran, Iran; 4Nanotechnology and Tissue Engineering Department, Stem Cell Technology Research Center, Tehran, Iran; 5Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran; 6Ophthalmology Department, Tehran University of Medical Sciences, Tehran, Iran; 7Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranAbstract: The study aimed to fabricate and characterize a 2-part artificial cornea as a substitute for penetrating keratoplasty in patients with corneal blindness. The peripheral part of the artificial cornea consisted of plasma-treated electrospun poly (ε-caprolactone (PCL nanofibers, which were attached to a hydrogel disc of polyvinyl alcohol (PVA as a central optical part. The physical properties of the prepared artificial cornea, including morphology, mechanical properties, light transmittance, and contact angle, were assessed. Cell attachment and proliferation studies were performed on rabbit limbal stem cells. The SEM image of the polymeric system showed that the peripheral part formed a highly porous scaffold that could facilitate tissue biointegration. Assessment of the mechanical properties of the peripheral nanofibrous part and the hydrogel optical part showed suitable elasticity. Young’s modulus values of the electrospun PCL skirt and PVA hydrogel core were 7.5 and 5.3 MPa, respectively, which is in line with the elasticity range of natural human cornea (0.3–7 MPa. The light transmittance of the central part was >85

  16. Estudo comparativo entre duas plataformas para realização de Lasik personalizado para correção de miopia e astigmatismo: Alcon CustomCornea® versus Bausch & Lomb Zyoptix® Wavefront-Guided Lasik for low to moderate myopia: CustomCornea® versus Zyoptix®

    Directory of Open Access Journals (Sweden)

    Telma Pereira Barreiro

    2009-08-01

    Full Text Available OBJETIVO: Comparar os resultados obtidos após o Lasik personalizado utilizando duas plataformas diferentes. MÉTODOS: Estudo prospectivo, randomizado com 50 pacientes míopes submetidos a cirurgia refrativa em ambos os olhos. Foram selecionados para o estudo, pacientes com equivalente esférico semelhante entre os olhos. Todos foram submetidos a Lasik bilateral e simultâneo, sendo que um olho foi operado pela plataforma CustomCornea® e o outro pela Zyoptix®. Acuidade visual sem e com correção, refração dinâmica e estática, medida das aberrações oculares, teste de sensibilidade ao contraste foram realizados no período pré-operatório e pós-operatório de 1, 3 e 6 meses. RESULTADOS: No período pré-operatório a média do equivalente esférico era de -3,29 ± 1,56 D no grupo CustomCornea® e de -3,22 ± 1,50 D no Zyoptix® (p=0,267. No sexto mês de pós-operatório, a média do equivalente esférico no grupo CustomCornea® era de -0,077 ± 0,23 D e -0,282 ± 0,30 D no Zyoptix® (p 20/20 foi alcançada em 86% dos olhos no grupo CustomCornea® e 70% no grupo Zyoptix® (p=0,094. Nenhum paciente perdeu duas ou mais linhas da melhor acuidade visual corrigida. Cem por cento dos olhos CustomCornea® e 88% dos Zyoptix® ficaram entre ± 0,50 D da emetropia (p=0,014*. Melhora da sensibilidade ao contraste em todas as frequências espaciais testadas foi observada em ambos os grupos. A aberração esférica apresentou aumento em ambos os grupos, porém este foi estatisticamente maior na plataforma Zyoptix® (pPURPOSE: To compare the visual and clinical outcomes of Wavefront-guided laser in situ keratomileusis (Lasik with Alcon CustomCornea® and Zyoptix® systems. METHODS: A prospective, randomized, masked and bilateral study was conducted. Fifty patients with preoperative spherical equivalent ranging from -1.00 to -6.50 D were enrolled for customized ablation in both eyes. All of them were submitted to Lasik CustomCornea® treatment in one

  17. Coloured cornea replacements with anti-infective properties: expanding the safe use of silver nanoparticles in regenerative medicine

    Science.gov (United States)

    Alarcon, E. I.; Vulesevic, B.; Argawal, A.; Ross, A.; Bejjani, P.; Podrebarac, J.; Ravichandran, R.; Phopase, J.; Suuronen, E. J.; Griffith, M.

    2016-03-01

    Despite the broad anti-microbial and anti-inflammatory properties of silver nanoparticles (AgNPs), their use in bioengineered corneal replacements or bandage contact lenses has been hindered due to their intense yellow coloration. In this communication, we report the development of a new strategy to pre-stabilize and incorporate AgNPs with different colours into collagen matrices for fabrication of corneal implants and lenses, and assessed their in vitro and in vivo activity.Despite the broad anti-microbial and anti-inflammatory properties of silver nanoparticles (AgNPs), their use in bioengineered corneal replacements or bandage contact lenses has been hindered due to their intense yellow coloration. In this communication, we report the development of a new strategy to pre-stabilize and incorporate AgNPs with different colours into collagen matrices for fabrication of corneal implants and lenses, and assessed their in vitro and in vivo activity. Electronic supplementary information (ESI) available: Collagen hydrogel, moulded as a cornea, prepared containing collagen protected AgNPs and representative images for collagen hydrogels, moulded as corneas, containing Blue AgNPs either unprotected or protected with LL37-SH; representative TEM images for Green-1 AgNPs prepared in this work; changes on surface plasmon band after synthesis for Green-2 AgNPs without LL37-SH; representative picture of the powder obtained for Green-1 AgNPs capped with LL37-SH after 72 h lyophilization, see main text; representative TEM images for Blue and Green-1 AgNPs prepared in this work; absorption spectra for the supernatants for collagen hydrogels containing Blue AgNPs; absorbance at 600 nm of PAO1 cultures prepared in 25% LB media incubated for 14 h at 37 °C in the presence of different concentrations of AgNPs, Green-1 or Blue, or silver nitrate; HECC cell density (cells per cm2) measured on gels with and without silver nitrate after 1 day of cell incubation; total silver concentration

  18. Corneal thickness changes during corneal collagen cross-linking with UV-A irradiation and hypo-osmolar riboflavin in thin corneas

    Directory of Open Access Journals (Sweden)

    Belquiz Amaral Nassaralla

    2013-06-01

    Full Text Available PURPOSE: To evaluate the thinnest corneal thickness changes during and after corneal collagen cross-linking treatment with ultraviolet-A irradiation, using hypo-osmolar riboflavin solution in thin corneas. METHODS: Eighteen eyes of 18 patients were included in this study. After epithelium removal, iso-osmolar 0.1% riboflavin solution was instilled to the cornea every 3 minutes for 30 minutes. Hypo-osmolar 0.1% riboflavin solution was then applied every 20 seconds for 5 minutes or until the thinnest corneal thickness reached 400 µm. Ultraviolet-A irradiation was performed for 30 minutes. During irradiation, iso-osmolar 0.1% riboflavin drops were applied every 5 minutes. Ultrasound pachymetry was performed at approximately the thinnest point of the cornea preoperatively, after epithelial removal, after iso-osmolar riboflavin instillation, after hypo-osmolar riboflavin instillation, after ultraviolet-A irradiation, and at 1, 6 and 12 months after treatment. RESULTS: Mean preoperative thinnest corneal thickness was 380 ± 11 µm. After epithelial removal it decreased to 341 ± 11 µm, and after 30 minutes of iso-osmolar 0.1% riboflavin drops, to 330 ± 7.6 µm. After hypo-osmolar 0.1% riboflavin drops, mean thinnest corneal thickness increased to 418 ± 11 µm. After UVA irradiation, it was 384 ± 10 µm. At 1, 6 and 12 months after treatment, it was 372 ± 10 µm, 381 ± 12.7, and 379 ± 15 µm, respectively. No intraoperative, early postoperative, or late postoperative complications were noted. CONCLUSIONS: Hypo-osmolar 0.1% riboflavin solution seems to be effective for swelling thin corneas. The swelling effect is transient and short acting. Corneal thickness should be monitored throughout the procedure. Larger sample sizes and longer follow-up are required in order to make meaningful conclusions regarding safety.

  19. Embryonic development of the cornea in the eye of the clearnose skate, Raja eglanteria: I. Stromal development in the absence of an endothelium

    Science.gov (United States)

    Conrad, G. W.; Paulsen, A. Q.; Luer, C. A.; Spooner, B. S. (Principal Investigator)

    1994-01-01

    Embryos of the clearnose skate, Raja eglanteria, develop in sea water at 20-22 degrees C, hatching after 82 +/- 4 days (Luer and Gilbert, Environ. Biol. Fishes, 13:161-171, 1985). Eyes develop as steadily enlarging spheres whose corneas have the same radius of curvature as the sclera. The cornea begins development as a 2-cell thick epithelium beneath which by Day 12 there is only a basal lamina and a wispy matrix separating it from the underlying lens. This matrix, modified by Day 16, is displaced on Day 22 by a few orthogonal plies of fibrillar primary stroma. Ply number increases to at least 13 by Day 30, reaching the final number of 20 +/- 2 by Day 42. Stromal fibroblasts (keratocytes) appear at the corneal periphery by Day 22, and in increased numbers by Day 30, a time at which no keratocytes are seen in the central stroma. However, by Day 40, many fibroblasts are present at the corneal periphery, invading the primary stroma between plies, occasionally reaching even the central cornea. By Day 53, keratocytes are present between all plies, from corneal periphery to center. Thickness of each ply in this secondary stroma increases, but the number of plies remains the same as in the primary stroma. Bowman's layer, non-invaded matrix beneath the epithelial basal lamina, is not evident until Day 53. Sutural fibers, first seen on Day 22, originate in the corneal epithelial basal lamina, traversing perpendicularly the plies of the primary stroma. Sutural fibers persist throughout development of the secondary stroma and into adulthood. In contrast to chicks, skate corneas remain transparent throughout development, and never form an endothelium.

  20. Localization of type V collagen and type IV collagen in human cornea, lung, and skin. Immunohistochemical evidence by anti-collagen antibodies characterized by immunoelectroblotting.

    OpenAIRE

    Konomi, H.; Hayashi, T.; NAKAYASU, K.; Arima, M.

    1984-01-01

    Tissue distribution of Type V collagen in comparison with Type IV collagen was investigated by indirect immunofluorescence microscopy. Affinity-purified rat antibodies to Type IV and Type V collagens obtained from human placenta reacted specifically only with the corresponding type of collagen in both native and denatured conformations. In indirect immunofluorescent stainings of human skin, lung, and cornea tissues, Type IV and Type V collagens showed distinct distributions. Type IV collagen ...

  1. New-Generation Hybrid Contact Lens for the Management of Extreme Irregularity in a Thin Cornea After Unsuccessful Excimer Laser Refractive Surgery

    OpenAIRE

    Piñero Llorens, David Pablo; Pérez Cambrodí, Rafael J.; Ruiz Fortes, Pedro; Blanes Mompó, Francisco J.

    2014-01-01

    Purpose: To report a very successful outcome obtained with the fitting of a new-generation hybrid contact lens of reverse geometry in a thin cornea with extreme irregularity due to the presence of a central island after unsuccessful myopic excimer laser refractive surgery. Methods: A 32-year-old man attended to our clinic complaining of very poor vision in his right eye after bilateral laser in situ keratomileusis (treatment or surgery) for myopia correction and some additional retreatments a...

  2. Reactive oxygen species (ROS)-generating oxidases in the normal rabbit cornea and their involvement in the corneal damage evoked by UVB rays

    Czech Academy of Sciences Publication Activity Database

    Čejková, Jitka; Štípek, S.; Crkovská, J.; Ardan, Taras; Midelfart, A.

    2001-01-01

    Roč. 16, - (2001), s. 523-533. ISSN 0213-3911 R&D Projects: GA ČR GV307/96/K226; GA ČR GA304/00/1635; GA MZd NG16 Institutional research plan: CEZ:AV0Z5039906 Keywords : ROS-generating oxidases * cornea Subject RIV: FF - HEENT, Dentistry Impact factor: 1.859, year: 2001

  3. Morphometry of cupromeronic blue-stained proteoglycan molecules in animal corneas, versus that of purified proteoglycans stained in vitro, implies that tertiary structures contribute to corneal ultrastructure.

    OpenAIRE

    Scott, J. E.

    1992-01-01

    Isolated, purified small chondroitin (dermatan) sulphate proteoglycans from corneas of cow and rabbit and cow sclera were stained with Cupromeronic blue in 'model' experiments. The lengths and thicknesses of the images were compared with those of the same proteoglycans stained in the tissue, using the critical electrolyte concentration principle to give specificity for sulphated proteoglycans, and keratanase 1 or chondroitinase ABC digestion to distinguish between chondroitin and keratan sulp...

  4. Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies.

    Science.gov (United States)

    Young, Robert D; Akama, Tomoya O; Liskova, Petra; Ebenezer, Neil D; Allan, Bruce; Kerr, Briedgeen; Caterson, Bruce; Fukuda, Michiko N; Quantock, Andrew J

    2007-01-01

    Keratan sulphate (KS) proteoglycans (PGs) are key molecules in the corneal stroma for tissue organisation and transparency. Macular corneal dystrophy (MCD) is a rare, autosomal recessive disease characterised by disturbances in KS expression. MCD is caused by mutations in CHST6, a gene encoding the enzyme responsible for KS sulphation. Sulphated KS is absent in type I disease causing corneal opacity and loss of vision. Genetic studies have highlighted the mutational heterogeneity in MCD, but supportive immunohistochemical studies on corneal KS have previously been limited by the availability of antibodies mostly reactive only with highly sulphated KS epitopes. In this study, we employed four antibodies against specific KS sulphation patterns, including one against unsulphated KS, to investigate their reactivity in a case of MCD compared with normal cornea using high-resolution immunogold electron microscopy. Mutation analysis indicated type I MCD with deletion of the entire open reading frame of CHST6. Contrast enhanced fixation revealed larger PG structures in MCD than normal. Unlike normal cornea, MCD cornea showed positive labelling with antibody to unsulphated KSPG, but was negative with antibodies to sulphated KSPG. These antibodies will thus facilitate high-resolution investigations of phenotypic heterogeneity in support of genetic studies in this disease. PMID:16944190

  5. Late Stage of Corneal Decompensation Caused by Progressive Keratoconus: Can We Treat It and Save the Cornea?

    Directory of Open Access Journals (Sweden)

    Igor Knezović

    2015-01-01

    Full Text Available Aim. To report a case of 40-year-old male with progressive bilateral keratoconus who had undergone transepithelial phototherapeutic keratectomy (TE-PTK and corneal collagen cross-linking (CXL using hypoosmolar riboflavin solution in a same day procedure. Methods. Eye examination showed that UCDVA on both eyes was 0,01 according to Snellen charts, and slit lamp biomicroscopy showed paracentral diffuse intrastromal corneal haze. Anterior OCT marked stromal hyperreflective zones and localized paracentral thinning of the cornea. Scheimpflug tomography noted keratoconus stages III-IV on both eyes. After 40/35 microns TE-PTK, a CXL was performed for 30 minutes using hypoosmolar riboflavin solution. The left eye was treated first and the right eye 1 month after. Follow-up period was 10 months. Results. One month after the treatment both eyes showed improvement in corneal topography and the UCDVA was better. Eight months after the treatment BSCVA improved to 0,6 in both eyes using Rose K2 contact lenses and remained stable. Conclusion. TE-PTK and CXL using hypotonic riboflavin solution as a same day procedure have been shown to be a safe and promising method in this case of progressive keratoconus. It was necessary to consider certain parameters that could influence the safety and the final outcome of this combined protocol.

  6. Dr John Nottingham's 1854 Landmark Treatise on Conical Cornea Considered in the Context of the Current Knowledge of Keratoconus.

    Science.gov (United States)

    Gokul, Akilesh; Patel, Dipika V; McGhee, Charles N J

    2016-05-01

    John Nottingham has been widely credited with the first accurate description of keratoconus in his treatise on conical cornea, published in 1854. Contained within the 270-page treatise are accounts and theories of keratoconus postulated by authors such as Scarpa, von Carion, von Ammon, and Mackenzie, synthesized by Nottingham in a treatise containing his own original observations. Nottingham's work delves deeply into keratoconus, with coverage reminiscent of a modern review, albeit in a far less succinct manner. He extensively describes the epidemiology, clinical presentation, underlying cause, and treatment of keratoconus. However, the concepts put forth are limited largely by the contemporary lack of understanding of the underlying anatomy and physiology of the eye, and the observations, by technological limitations. He postulates a similar treatment algorithm to that used today; optical devices being the management option of choice in the mild stages with surgery being a last resort. None of the surgical methods discussed are used in the modern era, but he does make reference to the possible efficacy of corneal transplantation. Nottingham's treatise was published over 160 years ago, yet his ideas and observations are surprisingly accurate. It is very possible that he was the first person to publish an accurate, comprehensive description of keratoconus. PMID:26989959

  7. Effect of the Multiglycoside of Tripterygium Wilfordii Hookf.(Tii)on Cornea Allograft Rejection Model in Rabbit

    Institute of Scientific and Technical Information of China (English)

    ZhijieLi; ChenLi

    1995-01-01

    Purpose:Toexamine the effect of Tii treatment of cornea graft survival in a rab-bit model.Methods:Tii was administrated orally after eccentrical corneal transplantation.Survival times were determined by biomicroscopy.Cytotoxic T lymphocytes(CTL)and delayed-type hypersensitivity(DTH)responses to donor alloantigens were assessed at ady 16after heterotopic corneal grafts.Results:Administration of Tii reduced the incidence and prologed the graft sur-vival time.Both CTLand DTH responses to donor alloantigens were severely ed-pressed in hosts treated with Tii.However,combination of Tii and cyclosporine further enhanced the immunosuppressive effects described above.Conclusions:Tii is a potent immunosuppressant with the ability to prolong allo-graft survival in the rabbit penetrating keratoplasty model and may have coordi-native effects with CsA through different mechanisms.Further studies are neces-sary to define any potentially coordinative role in the prevention of allograft rejec-tion in human keratoplasty.Eye Science 1995;11:168-172.

  8. Chronic Electromagnetic Exposure at Occupational Safety Level Does Not Affect the Metabolic Profile nor Cornea Healing after LASIK Surgery

    Directory of Open Access Journals (Sweden)

    David Crouzier

    2014-01-01

    Full Text Available LASIK eye surgery has become a very common practice for myopic people, especially those in the military. Sometimes undertaken by people who need to keep a specific medical aptitude, this surgery could be performed in secret from the hierarchy and from the institute medical staff. However, even though the eyes have been previously described as one of the most sensitive organs to electromagnetic fields in the human body, no data exist on the potential deleterious effects of electromagnetic fields on the healing eye. The consequences of chronic long-lasting radar exposures at power density, in accordance with the occupational safety standards (9.71 GHz, 50 W/m2, were investigated on cornea healing. The metabolic and clinical statuses after experimental LASIK keratotomy were assessed on the different eye segments in a New Zealand rabbit model. The analysis methods were performed after 5 months of exposure (1 hour/day, 3 times/week. Neither clinical or histological examinations, nor experimental data, such as light scattering, 1H-NMR HRMAS metabolomics, 13C-NMR spectra of lipidic extracts, and antioxidant status, evidenced significant modifications. It was concluded that withdrawing the medical aptitude of people working in electromagnetic field environments (i.e., radar operators in the navy after eye surgery was not justified.

  9. Evaluation of a Micro-Optical Coherence Tomography for the Corneal Endothelium in an Animal Model

    Science.gov (United States)

    Ang, Marcus; Konstantopoulos, Aris; Goh, Gwendoline; Htoon, Hla M.; Seah, Xinyi; Lwin, Nyein Chan; Liu, Xinyu; Chen, Si; Liu, Linbo; Mehta, Jodhbir S.

    2016-01-01

    Recent developments in optical coherence tomography (OCT) systems for the cornea have limited resolution or acquisition speed. In this study we aim to evaluate the use of a ‘micro-OCT’ (μOCT ~1 μm axial resolution) compared to existing imaging modalities using animal models of corneal endothelial disease. We used established cryoinjury and bullous keratopathy models in Sprague Dawley rats comparing ex vivo μOCT imaging in normal and diseased eyes to (1) histology; (2) in vivo confocal microscopy (IVCM); and (3) scanning electron microscopy (SEM). Qualitative and quantitative comparisons amongst imaging modalities were performed using mean endothelial cell circularity [(4π × Area)/Perimeter2] with coefficient of variation (COV). We found that μOCT imaging was able to delineate endothelial cells (with nuclei), detect inflammatory cells, and corneal layers with histology-like resolution, comparable to existing imaging modalities. The mean endothelial cell circularity score was 0.88 ± 0.03, 0.87 ± 0.04 and 0.88 ± 0.05 (P = 0.216) for the SEM, IVCM and μOCT respectively, with SEM producing homogenous endothelial cell images (COV = 0.028) compared to the IVCM (0.051) and μOCT (0.062). In summary, our preliminary study suggests that the μOCT may be useful for achieving non-contact, histology-like images of the cornea for endothelial cell evaluation, which requires further development for in vivo imaging. PMID:27416929

  10. Evaluation of a Micro-Optical Coherence Tomography for the Corneal Endothelium in an Animal Model.

    Science.gov (United States)

    Ang, Marcus; Konstantopoulos, Aris; Goh, Gwendoline; Htoon, Hla M; Seah, Xinyi; Lwin, Nyein Chan; Liu, Xinyu; Chen, Si; Liu, Linbo; Mehta, Jodhbir S

    2016-01-01

    Recent developments in optical coherence tomography (OCT) systems for the cornea have limited resolution or acquisition speed. In this study we aim to evaluate the use of a 'micro-OCT' (μOCT ~1 μm axial resolution) compared to existing imaging modalities using animal models of corneal endothelial disease. We used established cryoinjury and bullous keratopathy models in Sprague Dawley rats comparing ex vivo μOCT imaging in normal and diseased eyes to (1) histology; (2) in vivo confocal microscopy (IVCM); and (3) scanning electron microscopy (SEM). Qualitative and quantitative comparisons amongst imaging modalities were performed using mean endothelial cell circularity [(4π × Area)/Perimeter(2)] with coefficient of variation (COV). We found that μOCT imaging was able to delineate endothelial cells (with nuclei), detect inflammatory cells, and corneal layers with histology-like resolution, comparable to existing imaging modalities. The mean endothelial cell circularity score was 0.88 ± 0.03, 0.87 ± 0.04 and 0.88 ± 0.05 (P = 0.216) for the SEM, IVCM and μOCT respectively, with SEM producing homogenous endothelial cell images (COV = 0.028) compared to the IVCM (0.051) and μOCT (0.062). In summary, our preliminary study suggests that the μOCT may be useful for achieving non-contact, histology-like images of the cornea for endothelial cell evaluation, which requires further development for in vivo imaging. PMID:27416929

  11. Corneal collagen crosslinking for keratoconus. A review

    Directory of Open Access Journals (Sweden)

    M. M. Bikbov

    2014-10-01

    C, and collagen fiber diameter increase by 12.2 % (anterior stroma and 4.6 % (posterior stroma. In mild bullous keratopathy, corneal crosslinking provides antimicrobial effect. In moderate and severe keratopathy, crosslinking reduces pain and corneal edema and improves visual acuity immediately after the procedure. A case of HSV keratitis exacerbation was described. Amongst the complications, infection, halos, and posterior segment damage should be mentioned. Poor refractive results can be improved by the implantation of intrastromal corneal ring segments.

  12. 真菌毒素与角膜真菌感染%Mycotoxins and fungal infection of cornea

    Institute of Scientific and Technical Information of China (English)

    吴福进; 胡建章; 韩晓丽

    2013-01-01

    Mycotoxins are secondary metabolites produced by the toxigenic fungi in suitable environmental conditions,the main toxicity of which are carcinogenesis,genotoxicity,teratogenesis,hepatotoxicity and immunosuppression.After fungal infects the cornea,it may release mycotoxins to cause corneal damage.However what roles do the mycotoxins play in fungal keratitis is still unclear.The biosynthesis of mycotoxinis is regulated by a series of related function genes ; in addition,pH value,carbon nitrogen ratio and other environmental conditions are the influence factors as well.This article reviews the common harms,regulatory factors and their possible roles in the occurrence and development of keratomycosis.%真菌毒素(Mycotoxin)是由产毒真菌在适宜的环境条件下产生的具有毒性的次级代谢产物,主要毒性有致癌作用、遗传毒性、致畸作用、肝细胞毒性和免疫抑制等.真菌感染角膜后可能释放真菌毒素导致角膜损害,但至今对真菌毒素在真菌性角膜炎中究竟扮演什么角色,仍不明确.真菌毒素的生物合成受到一系列相关功能基因的调控;此外,pH值、碳氮比等环境条件也能影响其产量.本文就常见的真菌毒素的危害、调控因素及其可能在真菌性角膜炎发生发展中的作用进行综述.

  13. Expression of c-Fos and c-Jun in the cornea, lens, and retina after ultraviolet irradiation of the rat eye and effect of topical antisense oligodeoxynucleotides

    International Nuclear Information System (INIS)

    Aims - Immunohistochemical techniques were used to investigate c-Fos and c-Jun proto-oncogene expression in the cornea, lens, and retina after ultraviolet irradiation of the rat eye. Methods -Eyes of anaesthetised rats were exposed to 1.5 J/cm2 of ultraviolet radiation (280-380 nm). Animals were perfused 1, 6, or 24 hours after irradiation and tissue sections were incubated with specific antiserum to c-Fos and c-Jun, respectively. Non-irradiated contralateral eyes displayed no c-Fos and c-Jun immunoreactivity. One and 6 hours after ultraviolet exposure numerous c-Fos and c-Jun immunopositive nuclei were observed mainly in the epithelial cell layers of the cornea and the lens epithelium. Scattered labelled nuclei were detectable in the retinal ganglion cell layer and the inner nuclear layer. Twenty four hours after irradiation c-Fos and c-Jun protein expression returned to near control levels. Histological signs of ultraviolet damage (for example, chromatin condensation, nuclear fragmentation) were first recognisable in the corneal epithelium 6 hours after irradiation and became more apparent at later times. The rapid and sustained activation of c-Fos and c-Jun expression in the eye after single ultraviolet exposure may represent the molecular mechanism underlying ultraviolet induced photodamage and initiation of cell death. Furthermore, topical application of a c-fos antisense oligode-oxynucleotide to the ultraviolet exposed rat eye inhibited the increase in c-Fos expression in the cornea, suggesting therapeutic activity of antisense drugs in corneal malignant and infectious diseases. (author)

  14. Clinical assessment of frequency and structure of subepithelial fibroplasias of a cornea after a photorefractive keratectomy in patients with refraction anomalies

    Directory of Open Access Journals (Sweden)

    V. V. Egorov

    2014-07-01

    Full Text Available Purpose: To investigate the frequency and structure of a subepithelial fibroplasia of a cornea after photorefractive keratectomy concerning myopia correction.Methods: 170 patients (337 eyes with the following refraction anomalies: stationary myopia from 1.5 to 13.0 D with astigmatism from 0.75 to 4.25 D. Frequency of survey in the postoperative period — in 1, 3, 6, 12 months. To all patients Dexamethasone instillation according to the decreasing scheme was carried out. Existence of biomicroscopic signs of any degrees of subepithelial fibroplasias of a cornea, existence and extent of loss of uncorrected visual acuity in comparison with initial, regress of refractive effect of operation were taped. Subjective estimation of quality of vision was made by patients in 1 year after operations.Results: Subepithelial fibroplasias with intensity 1.0‑2.5 points stopped in whole or in part after additional treatment, were surveyed as clinically significant and made 4,75 % (16 eyes, 9 patients. Terms of their emergence: from 1 to 10 months after operation. At 3 patients (6 eyes intensity of fibroplasias made 2.0‑2.5 points before treatment and 1.0‑2.5 points after it. To the observation extremity 7 patients from this group had visual complaints, a discomfort in an eye. Indicators of Subjective estimation of quality of vision in patients with clinically significant fibroplasias of a cornea varied from 5 to 2.9 points.Conclusion: Frequency of clinically significant fibroplasias after photorefractive keratectomy made 4.75 %. After treatment theirintensity made 1.5‑2.5 points only in 4 eyes (2 patients.

  15. Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

    OpenAIRE

    Kaluzhny, Yulia; Kandárová, Helena; d’Argembeau-Thornton, Laurence; Kearney, Paul; Klausner, Mitchell

    2015-01-01

    To comply with the Seventh Amendment to the EU Cosmetics Directive and EU REACH legislation, validated non-animal alternative methods for reliable and accurate assessment of ocular toxicity in man are needed. To address this need, we have developed an eye irritation test (EIT) which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model that is based on normal human cells. The EIT is able to separate ocular irritants and corrosives (GHS Categories 1 and 2 ...

  16. Conhecimento de estudantes de medicina sobre o processo de doação de córneas The knowledge of medical students about cornea donation

    Directory of Open Access Journals (Sweden)

    Rodrigo França de Espíndola

    2007-08-01

    Full Text Available OBJETIVO: Avaliar o conhecimento de estudantes de medicina sobre o processo de doação de córneas, comparando-o entre aqueles que já cursaram a disciplina de Oftalmologia com os demais. MÉTODOS: Foi aplicado um questionário a estudantes de medicina do interior de São Paulo, contendo dados como: idade, sexo, ano de graduação e 10 perguntas de múltipla escolha sobre o tema. As questões abordaram o limite de idade para doação, contra-indicações para o transplante, tempo para retirada das córneas, dentre outras. Foi comparado o conhecimento (% de acertos entre aqueles que haviam cursado a disciplina de Oftalmologia (Grupo A com os demais (Grupo B. RESULTADOS: Foram entrevistados 402 estudantes. Destes, 140 eram do grupo A e 262 do grupo B. Não houve diferença estatisticamente significante entre os dois grupos (p=0,8328. CONCLUSÃO: O conhecimento sobre o processo de doação de córneas mostrou-se insuficiente entre os alunos entrevistados, mesmo para aqueles que haviam cursado a disciplina de Oftalmologia, apontando a necessidade de aperfeiçoamento do conhecimento divulgado nas escolas médicas.PURPOSE: To compare knowledge of medical students about the cornea donation process among those who already studied Ophthalmology and the others. METHODOS: A questionnaire containing data as: age, sex, graduation year, and 10 multiple-choice questions about the subject was applied to medical students from the interior of São Paulo state, Brazil. The questions were: age for donation, contraindication for transplants, the time limit to remove the cornea, among others. The knowledge regarding cornea donation was compared between the two groups: students who already studied Ophthalmology (Group A and the others (Group B. RESULTS: The study group was composed of 402 students, of whom 140 were of group A and 262 of group B. Knowledge between the two groups was different, but not statistically significant (p=0.8328. CONCLUSION: Knowledge about

  17. Ocular lesions associated with attachment of the copepod Ommatokoita elongata (Lernaeopodidae: Siphonostomatoida) to corneas of Pacific sleeper sharks Somniosus pacificus captured off Alaska in Prince William Sound.

    Science.gov (United States)

    Benz, George W; Borucinska, Joanna D; Lowry, Lloyd F; Whiteley, Herbert E

    2002-06-01

    Twenty eyes from 10 Pacific sleeper sharks Somniosus pacificus, infected with the copepod Ommatokoita elongata, were collected in Prince William Sound, Alaska, and the eyes of an additional 18 S. pacificus captured in the same area were inspected for copepods. Prevalence of infection by adult female O. elongata was 97% (n = 28); mean intensity of infection was 1.89 (+/-1SD = 0.32) adult female copepods per infected shark and 1.0 (+/- 1SD = 0.0) adult female copepods per infected eye. Five of the 20 collected eyes were infected by O. elongata chalimi, and 9 of 20 eyes had 1 to several remnants of bullae embedded in the cornea. Bullae were each associated with a corneal opacity, and anchoring plugs of chalimi were associated with pinpoint lesions in the cornea or conjunctiva. All eyes exhibited marked edema and erosion of the bulbar conjunctiva, and this torus-shaped lesion corresponded to each O. elongata adult female's presumed feeding and abrasion radius. Histological examinations revealed lesions in the anterior segment of eyes to be generally similar, but graded, in severity, and in all eyes they involved the conjunctiva, cornea, filtration angle, and iris. Epithelial lesions were characterized by corneal ulceration, dysplasia, hyperplasia, and heterophilic keratitis, and by ulcerative conjunctivitis accompanied by epithelial hyperplasia with rete peg formation. Disorganization of fibers, necrosis, mineralization, minimal heterophilic influx, and perilimbic neovascularization were associated with bullae in the corneal stroma. Within the limbus there was diffuse histiocytic and lymphocytic inflammation and marked lymphofollicular hyperplasia. Heterophilic and mononuclear anterior uveitis affecting the filtration angle and anterior surface of the iris was also observed in most eyes. One eye had a partial transcorneal prolapse of a ruptured lens, with degenerative changes in the ruptured lens and severe keratitis associated with the anchoring devices of an adult

  18. Invasive pulmonary aspergillosis in autoimmune bullous diseases patients:three cases report%自身免疫性大疱病合并侵袭性肺曲霉病:3例报告

    Institute of Scientific and Technical Information of China (English)

    余进; 牟向东; 万喆; 阙呈立; 李若瑜

    2012-01-01

    Objective To study the diagnosis and treatment of invasive pulmonary aspergillosis in autoimmune bullous diseases patients under long-term glucocorticoids treatment by retrospectively reviewing three cases. Methods Report three cases of autoimmune bulious diseases with invasive pulmonary aspergillosis. KOH smear and culture, antigen detection, chest CT scan and his-topathologic test were used to diagnose the patients. The organisms isolated from the sputum were identified as Aspergillus fumigatus A. Flavus and Emericella echinulata by morphological and molecular methods. Results Three cases were diagnosed as invasive pulmonary aspergillosis. The patients were cured with voriconazole treatment and other antifungal agents. Conclusion Autoimmune bullous diseases patients should be watched out emerging invasive apergillosis during long-term glucocorticoids therapy. Combined laboratory examinations will be helpful in early diagnosis and increasing efficacy.%目的 通过典型病例回顾探讨自身免疫性大疱病糖皮质激素治疗过程中合并肺曲霉病的诊断和治疗情况.方法 报告3例自身免疫性大疱病合并侵袭性肺曲霉病病例.3例病例均行痰镜检、培养、抗原检测、胸部CT或者坏死组织病理检查.分离病原菌经形态学和分子生物学鉴定为烟曲霉、黄曲霉和刺孢裸胞壳.结果 3例病例证实为侵袭性肺曲霉病.进行以伏立康唑为主的综合治疗后均治愈.结论 自身免疫性大疱病糖皮质激素治疗过程中应警惕肺曲霉病的发生.多种实验室检查可以帮助早期诊断,提高疗效.

  19. Commercially available rigid gas-permeable contact lens for protecting the cornea from drying during vitrectomy with a wide viewing system

    Directory of Open Access Journals (Sweden)

    Kamei M

    2012-08-01

    Full Text Available Motohiro Kamei, Nagakazu Matsumura, Hirokazu Sakaguchi, Yusuke Oshima, Yasushi Ikuno, Kohji NishidaDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Osaka, JapanPurpose: We evaluated the usefulness of commercially available materials for protecting the cornea from drying during vitrectomy with a wide-angle viewing system.Methods: Three vitreoretinal surgeons evaluated fundus visibility during vitrectomy on the images of five study materials used on the cornea: balanced saline solution, a viscoelastic agent, a vitrectomy contact lens, a rigid gas permeable (RGP contact lens, and a soft contact lens. Fundus visibility was graded using the following scale: grade 1, unclear; grade 2, slightly unclear; grade 3, clear; and grade 4, very clear.Results: Respective scores for each material used during core vitrectomy and membrane removal were: balanced saline solution, 3.3/3.3; viscoelastic agent, 3.8/3.3; vitrectomy contact lens, 3.3/4.0; RGP contact lens, 4.0/3.9; soft contact lens, 1.1/not applicable.Conclusion: The RGP contact lens provided visibility similar to or clearer than that obtained with other methods. Because of its ease of use and lower cost, use of the RGP contact lens is ideal during vitrectomy performed with a wide-angle viewing system.Keywords: contact lens, RGP, viscoelastic agent, vitrectomy, wide viewing system

  20. Biofiltration of fishpond effluents and accumulation of N-compounds (phycobiliproteins and mycosporine-like amino acids) versus C-compounds (polysaccharides) in Hydropuntia cornea (Rhodophyta)

    International Nuclear Information System (INIS)

    The biofiltration capacity, biomass-yield and accumulation of N- and C-compounds of Hydropuntia cornea were analyzed. Algae were grown in different conditions for 28 d: outdoor and indoor, with or without fishpond effluents. N-uptake efficiency of these effluents was higher than 95% after 7 d both outdoors and indoors. N-enriched conditions reduced the extent of photoinhibition and increased the maximal quantum yield in H. cornea. The biomass-yield was higher in outdoor grown-algae after 7 d and decreased independently of the treatment after 28 d. N, acid polysaccharide (AP) and mycosporine-like amino acid (MAA)-yields decreased throughout the experiment in all conditions. The highest MAA-yield was observed in fishpond effluent outdoor-grown algae, indicating a positive effect of increased radiation on MAA accumulation. However, APs were higher under N-depleted conditions. The use of MAAs as UV-screening and antioxidants, and the use of AP as immunostimulants are discussed.

  1. The management of cornea blindness from severe corneal scarring, with the Athens Protocol (transepithelial topography-guided PRK therapeutic remodeling, combined with same-day, collagen cross-linking

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    Kanellopoulos AJ

    2012-02-01

    Full Text Available Anastasios John KanellopoulosLaservision.gr Institute, Athens, Greece; Manhattan Eye, Ear and Throat Hospital, New York, NY, USA; New York University Medical School, New York, NY, USAPurpose: To evaluate the safety and efficacy of combined transepithelial topography-guided photorefractive keratectomy (PRK therapeutic remodeling, combined with same-day, collagen cross-linking (CXL. This protocol was used for the management of cornea blindness due to severe corneal scarring.Methods: A 57-year-old man had severe corneal blindness in both eyes. Both corneas had significant central scars attributed to a firework explosion 45 years ago, when the patient was 12 years old. Corrected distance visual acuity (CDVA was 20/100 both eyes (OU with refraction: +4.00, –4.50 at 135° in the right eye and +3.50, –1.00 at 55° in the left. Respective keratometries were: 42.3, 60.4 at 17° and 35.8, 39.1 at 151.3°. Cornea transplantation was the recommendation by multiple cornea specialists as the treatment of choice. We decided prior to considering a transplant to employ the Athens Protocol (combined topography-guided partial PRK and CXL in the right eye in February 2010 and in the left eye in September 2010. The treatment plan for both eyes was designed on the topography-guided wavelight excimer laser platform.Results: Fifteen months after the right eye treatment, the right cornea had improved translucency and was topographically stable with uncorrected distance visual acuity (UDVA 20/50 and CDVA 20/40 with refraction +0.50, –2.00 at 5°. We noted a similar outcome after similar treatment applied in the left eye with UDVA 20/50 and CDVA 20/40 with –0.50, –2.00 at 170° at the 8-month follow-up.Conclusion: In this case, the introduction of successful management of severe cornea abnormalities and scarring with the Athens Protocol may provide an effective alternative to other existing surgical or medical options.Keywords: Athens Protocol, collagen cross

  2. Tonometry in normal and scarred corneas, and in postkeratoplasty eyes : A comparative study of the Goldmann, the ProTon and the Schiotz tonometers.

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    Jain Arun

    2000-01-01

    Full Text Available PURPOSE: Clinical comparison of intraocular pressure (IOP measured with the Goldmann applanation tonometer (GAT, the ProTon tonometer (PT, and the Schiotz tonometer (ST, in normal eyes, eyes with scarred corneas and postkeratoplasty eyes. MATERIAL AND METHODS: The IOP readings with GAT, PT, and ST were compared in 125 eyes with normal corneas (Group A, 17 eyes with scarred corneas (Group B, and in 21 postkeratoplasty eyes (Group C. The data were statistically analysed at 95% confidence interval; linear regression analysis and paired t-test were done. RESULTS: The mean differences and their standard deviation [SD] between GAT and PT readings, and GAT and ST readings respectively were: [1] in Group A: -0.23 [SD 2.75] mmHg and +0.24 [SD 3.18] mmHg respectively; [2] in Group B: -1.8 [SD 12.67] mmHg and -4.5 [SD 9.95 mmHg; and [3] in Group C: +0.24 [SD 8.72] mmHg and -0.12 [SD 8.7] mmHg. They were not statistically significant. In Group A the 95% confidence interval between GAT and PT readings was -5.27 mmHg to 5.73 mmHg, and between GAT and ST readings, -6.12 mmHg to 6.59 mmHg. Ninety six [77%] eyes with the PT and 84 [69%] eyes with ST measurements were within 3 mmHg of GAT pressure. The correlation coefficients [r] for PT and ST were 0.93 [P = 0.0000] and 0.88 [P = 0.0000] respectively. In Group B 95% confidence interval between GAT and PT readings was -27.17 mmHg to 23.51 mmHg, and between GAT and ST measurement, -24.37 mmHg to 15.44 mmHg. The correlation coefficients [r] for the PT and ST were 0.112 [P = 0.660] and 0.630 [P = 0.006] respectively. In group C, the 95% confidence interval between GAT and PT measurements was -17.20 mmHg to 17.67 mmHg, and between GAT and ST measurements, -17.51 mmHg to 17.27 mmHg. The correlation coefficients [r] for the PT and the ST were 0.780 [P = 0.0000] and 0.740 [P = 0.0001] respectively. CONCLUSIONS: In clinical practice PT appears to have a higher level of accuracy than ST in normal corneas. In scarred

  3. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model

    Science.gov (United States)

    Maenz, Martin; Morcos, Mourice

    2011-01-01

    Purpose To establish a cornea transplant model in a pigmented rat strain and to define the immunologic reaction toward corneal allografts, by studying the cellular and humoral immune response after keratoplasty. Methods Full thickness penetrating keratoplasty was performed on Brown Norway (RT1n) recipients using fully major histocompatibility complex (MHC)-mismatched Piebald-Viral-Glaxo (PVG; RT1c) donors. Using multicolor flow cytometry (FACS) we quantified and compared the cellular composition of draining versus non-draining lymph nodes (LN). Furthermore, we developed an isolation method to release viable graft infiltrating lymphocytes (GIL) and subjected them to phenotypic analysis and screened serum from transplanted animals for allo-antibodies. Results Assessing ipsi-lateral submandibular LN we find ample evidence for post surgical inflammation such as elevated absolute numbers of cluster of differentiation (CD)4+, CD8+, B-cells, and differential expression of CD134. However, we could not unequivocally identify an allo-antigen-specific immune response. FACS analysis of lymphocytes isolated from collagenase digested rejected corneas revealed the following six distinct subpopulations: MHC-2+ cells, CD4+ T-cells, CD8+ T-cells, CD161dull large granular lymphocytes, CD3+ CD8+ CD161dull natural killer (NK)-T-cells and CD161high CD3- NK cells. At post-operation day (POD)-07 only CD161dull MHC-2neg large granular lymphocytes (LGLs) were detected in syngeneic and allo-grafts. In concordance with an increase in B-cell numbers we often detected copious amounts of allo-antibodies in serum of rejecting animals, in particular immunoglobulin (Ig) M (IgM), immunoglobulin (Ig) G1 (IgG1), and IgG2a. Conclusions Our results demonstrate that despite its immune privileged status and low-responder characteristics of the strain combination, allogeneic corneal grafts mount a full fledged T helper1 (Th1) and Th2 response. The presence of NK-T-cells and NK-cells in rejecting corneas

  4. Estimation of Postmortem Interval by Detecting Thickness of Cornea Using Ultrasonic Method%超声法测量角膜厚度推断死亡时间

    Institute of Scientific and Technical Information of China (English)

    吕国丽; 姜富学; 许心舒; 蒋拥军; 李志刚; 王欣; 石河; 余礼聪; 许传超

    2012-01-01

    目的 用超声法测量角膜厚度,探索其死后变化规律.方法 11只家兔随机分为两组,分别为角膜上皮完整组及去角膜上皮组.后者采用机械刮除法去除角膜上皮.利用超声测厚仪连续监测两组动物的死后多个时间点的角膜厚度,并对角膜厚度变化与死亡时间进行相关回归分析.结果 超声法测量角膜上皮完整组死后兔角膜厚度的变化值与死亡时间呈非线性相关,且具有较强的相关性,相关系数为0.922,而去角膜上皮组的相关系数为0.822.结论 超声法测量角膜厚度对推断早期死亡时间具有较可靠的应用前景.完整的角膜上皮对于超声测量角膜厚度具有关键作用.%Objective To explore the postmortem changes of cornea thickness measured by ultrasonic pachymetry. Methods Eleven rabbits were randomly divided into two groups: one group with intact corneal epithelium and another group without intact corneal epithelium. In the later group, the corneal epithelium of the rabbit was scraped using mechanical elimination method. The corneal thickness was monitored continuously by ultrasonic pachymetry at several postmortem interval points in rabbits of the two groups. The changes of corneal thickness and postmortem interval were explored by relative regression analysis. Results The thickness of the cornea showed a strong non-linear correlation with the postmortem interval in the group with intact corneal epithelium. The group with intact corneal epithelium showed the correlation coefficient 0.922 and the group without intact corneal epithelium showed the correlation coefficient 0.822, respectively. Conclusion The corneal thickness measured by ultrasonic pachymetry shows a potential value for estimating early postmortem interval. The intact corneal epithelium is a crucial factor for the measurement of cornea thickness by ultrasonic pachymetry.

  5. Combined DSEK and Transconjunctival Pars Plana Vitrectomy

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    Mona Sane

    2016-01-01

    Full Text Available We report here three patients who underwent combined Descemet’s stripping with endothelial keratoplasty and transconjunctival pars plana vitrectomy for bullous keratopathy and posterior segment pathology. A surgical technique and case histories are described. Anatomic and visual outcomes of combined Descemet’s stripping with endothelial keratoplasty and vitrectomy were excellent. Our experience provides technical guidelines and limitations. The combined minimally invasive techniques allow for rapid anatomical recovery and return of function and visual acuity in a single sitting.

  6. Outcome of corneal transplantation in a private institution in Saudi Arabia

    Science.gov (United States)

    Omar, Nazri; Chacra, Charbel T Bou; Tabbara, Khalid F

    2013-01-01

    Background The aim of this work was to describe the indications, complications, and outcomes of penetrating keratoplasty (PKP) in Saudi Arabia. Methods In a retrospective, noncomparative interventional case series, the medical records of patients who underwent PKP from January 2000 to December 2008 and had a minimum follow-up of 6 months were reviewed. All corneas were obtained from eye banks in the US. Indications, complications, and outcomes of surgery were recorded. This study was approved by the institutional review board. Results Eighty-five consecutive eyes were included in this study. There were 52 (61.2%) males and 33 (38.8%) females. The median age was 35.0 years (range 3–85 years), and the median follow-up period was 24 months (range 6–108 months). The indications for PKP were keratoconus, bullous keratopathy, corneal scars, corneal dystrophy, and corneal regraft. The overall graft survival time was 88.9 months ± 4.9 months (mean ± standard error of mean, 95% confidence interval [CI] 79.4 months –98.4 months) while the 3-year and 5-year cumulative survival rates were 90.7% and 84.3%, respectively. Surgical indication (P = 0.038), immune rejection (P < 0.001), preoperative corneal vascularization (P = 0.022), and perioperative high intraocular pressure (P = 0.032) were associated significantly with corneal graft failure in univariate analysis. Multivariate analysis reduced these significant associations to rejection (P < 0.001) and vascularization (P = 0.009). Relative risk for failure in rejected cornea was 16.22 (95% CI 4.99–52.69) and in vascularized cornea was 3.89 (95% CI 1.36–11.09). At last visit following PKP, 34 (40%) eyes had best spectacle-corrected visual acuity of 20/40 or better, and 51 (60.0%) eyes had 20/80 or better. Best spectacle-corrected visual acuity was worse than 20/400 in 15 (17.6%) eyes. Conclusion The overall corneal graft survival in a private setting in Saudi Arabia can be excellent. Thorough preoperative evaluation

  7. Confocal microscopy findings in deep anterior lamellar keratoplasty performed after Descemet's stripping automated endothelial keratoplasty

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    Pang A

    2014-01-01

    Full Text Available Audrey Pang,1,2 Karim Mohamed-Noriega,1 Anita S Chan,1,3–5 Jodbhir S Mehta1,3 1Singapore National Eye Centre, 2Department of Ophthalmology, Tan Tock Seng Hospital, 3Singapore Eye Research Institute, 4Department of Histopathology, Pathology, Singapore General Hospital, 5Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore Background: This study describes the in vivo confocal microscopy findings in two patients who had deep anterior lamellar keratoplasty (DALK following Descemet's stripping automated endothelial keratoplasty (DSAEK. Methods: The study reviewed the cases of two patients who first underwent DSAEK followed by DALK when their vision failed to improve due to residual stromal scarring. In the first case, a DSAEK was performed for a patient with pseudophakic bullous keratopathy. After surgery, the patient's vision failed to improve satisfactorily due to residual anterior stromal opacity and irregularity. Subsequently, the patient underwent a DALK. The same two consecutive operations were performed for a second patient with keratoconus whose previous penetrating keratoplasty had failed and had secondary graft ectasia. In vivo confocal microscopy was performed 2 months after the DALK surgery in both cases. Results: At 3 months after DALK, the best-corrected visual acuity was 6/30 in case 1 and 6/24 in case 2. In vivo confocal microscopy in both cases revealed the presence of quiescent keratocytes in the stroma layers of the DSAEK and DALK grafts, which was similar in the central and peripheral cornea. There was no activated keratocytes or haze noted in the interface between the grafts. Conclusion: Our short-term results show that performing a DALK after a DSAEK is an effective way of restoring cornea clarity in patients with residual anterior stromal opacity. In vivo confocal microscopy showed that there were no activated keratocytes seen in the interface of the grafts, which suggests

  8. Topical Anesthetic Abuse Keratopathy and its Clinical Progression

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    Volkan Dayanır

    2013-08-01

    Full Text Available To report a case series with topical anesthetic abuse and to define the clinical progression of the disease. Six eyes of three topical anesthetic abuser patients with various corneal abnormalities are reviewed. Signs of the disease are presented in order to document the natural progression of the disease. A report was filed to the General Directorate of Pharmaceuticals and Pharmacy, Ministry of Health, Ankara, Turkey describing the outcome of uncontrolled use of topical anesthetic. Topical anesthetic abuse can result in serious corneal complications such as corneal epithelium defect, ring keratitis, stromal infiltrate, hypopyon, superficial and deep corneal vascularization, corneal lysis, and at last spontaneous perforation. The General Directorate of Pharmaceuticals and Pharmacy issued that topical anesthetics can only be prescribed by an ophthalmologist up to four boxes a month, the prescription must be kept for one, year and criminal action will be taken against pharmacies disregarding these provisions. (Turk J Ophthalmol 2013; 43: 289-93

  9. 组织工程角膜三维构建的实验研究%Three-dimensional construction of tissue-engineered cornea in vitro

    Institute of Scientific and Technical Information of China (English)

    庞鵾鹏; 张凯; 朱婧; 鞠成群; 吴欣怡

    2016-01-01

    目的 以人胚胎干细胞(hESC)诱导细胞为种子细胞,以脱细胞猪角膜基质(APCM)为支架三维构建生物工程角膜,以期用于穿透性角膜移植,解决角膜供体极度匮乏的难题.方法 实验研究.无菌条件下将新鲜猪角膜组织置于0.5% SDS溶液中4℃脱细胞24 h,获取APCM.将hESCs与人角膜基质细胞通过Transwell共培养5d,获取眼周间充质干细胞(POMPs),再于人晶状体上皮细胞源性条件培养基继续培养14 d获取角膜内皮样细胞并进行鉴定和筛选纯化.将纯化后扩增的角膜内皮样细胞接种于APCM构建角膜内皮植片,并移植入角膜内皮功能失代偿动物模型进行泵功能评估;采用人角膜缘干细胞(LSCs)来源的条件培养基培养hESCs 12 d,诱导其分化人角膜上皮样细胞并筛选鉴定,将其与APCM构建的角膜上皮植片移植于LSC失代偿动物模型的角膜缘,观察其眼表修复能力.结果 诱导的人角膜内皮样细胞表达内皮细胞相关标记物vimentin、N-cadhefin、Na+/K+ATP酶和ZO-1.构建的角膜内皮植片能够促使角膜内皮功能失代偿动物的角膜逐渐恢复透明.构建的角膜上皮细胞植片具有4~5层细胞复层结构,类似于正常角膜上皮,且能够一定程度上修复LSC失代偿动物模型眼表.结论 采用hESCs诱导分化来源的细胞与APCM构建的人角膜内皮植片和人角膜上皮植片具有类似于正常角膜的功能,为全层生物角膜的构建提供了良好的实验和理论基础,具有良好的临床应用前景.%Objective To develop a corneal replacement for penetrating keratoplasty (PKP).A three-dimentional,tissue-engineered cornea is constructed by using human embryonic stem cells (hESCs) derived corneal endothelial-like cells and corneal epithelial-like cells as seed cells,and acellular porcine cornea matrix (APCM) as scaffold.Methods Experimental study.To obtain APCM,sterile fresh porcine cornea was decellularized with a 0.5% SDS

  10. Unusually delayed presentation of persistent Descemet’s membrane tear and detachment after cataract surgery

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    Morkin MI

    2014-08-01

    Full Text Available Melina I Morkin,1,2 Rehan M Hussain,2 Ryan C Young,2 Tracy Ravin,2 Sander R Dubovy,2 Eduardo C Alfonso2 1Department of Ophthalmology, Shiley Eye Center, University of California – San Diego, San Diego, CA, 2Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA Abstract: A 51-year-old male who had undergone phacoemulsification in his left eye 11 months prior presented with complaint of sudden onset of blurred vision in the same eye. Review of his clinical course, slit-lamp exam, pachymetry, and specular endothelial microscopy led to the diagnosis of acute hydrops caused by Descemet’s membrane dehiscence at the site of the incision. He was initially managed with medical treatment and observation. In the subsequent months of follow-up, the corneal edema and the patient’s visual acuity did not improve. Intracameral gas injection was performed 7 months after presentation, but because of persistent corneal edema and nonattached Descemet’s membrane, penetrating keratoplasty was performed. Histopathologic examination confirmed the diagnosis. The patient has had a clear corneal graft since then. Although Descemet’s membrane detachment is a rather common complication after intraocular surgery, its unusually delayed presentation can also occur, and should not be confused with pseudophakic bullous keratopathy. Many mechanisms have been studied for the development of early tears and detachments after cataract surgery, but little is known about late presentations. The authors explore possible causes, and highlight the importance of instructing patients to avoid eye rubbing and any other type of trauma to the cornea after intraocular surgery. Keywords: Descemet’s membrane tear, detachment, cataract surgery, phacoemulsification

  11. Tarsorrafia: aplicações em um Serviço de Córnea Tarsorrhaphy: applications in a Cornea Service

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    Patrick Frensel de Moraes Tzelikis

    2005-02-01

    were evaluated retrospectively. Reviewed data included patient's sex and age, indication for tarsorrhaphy, duration of signs and symptoms before tarsorrhaphy, time for epithelial healing after tarsorrhaphy, type of tarsorrhaphy, complications, follow-up time. RESULTS: Eighteen patients underwent tarsorrhaphy. The indications for a tarsorrhaphy were exposure keratopathy (27.8%, persistent epithelial defect associated with penetrating keratoplasty (38.8%, neurotrophic ulcer (11.1%, dry eye syndrome (5.6%, Stevens-Johnson syndrome (11.1%, and chemical burn (5.6%. The epithelial defect resolved completely in 15 patients (83.3%. Mean duration of signs and symptoms before tarsorrhaphy was 98.7±48.6 days, and time to heal after tarsorrhaphy was 53.2±22.8 days. Of the 18 procedures, two (11.1% were temporary and 16 (88.9% permanent. Complications after tarsorrhaphy were premature opening of the tarsorrhaphy, trichiasis and pyogenic granuloma. CONCLUSION: Tarsorrhaphy is a simple procedure, being also very safe and effective in the management of nonhealing epithelial defects, with a success rate of 83.3% and with minor complications.

  12. The generation of 4-hydroxynonenal, an electrophilic lipid peroxidation end product, in rabbit cornea organ cultures treated with UVB light and nitrogen mustard

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Ruijin; Po, Iris; Mishin, Vladimir; Black, Adrienne T. [Pharmacology and Toxicology, Rutgers University, Piscataway, NJ (United States); Heck, Diane E. [Environmental Science, New York Medical College, Valhalla, NY (United States); Laskin, Debra L. [Pharmacology and Toxicology, Rutgers University, Piscataway, NJ (United States); Sinko, Patrick J. [Pharmaceutics, Rutgers University, Piscataway, NJ (United States); Gerecke, Donald R.; Gordon, Marion K. [Pharmacology and Toxicology, Rutgers University, Piscataway, NJ (United States); Laskin, Jeffrey D., E-mail: jlaskin@eohsi.rutgers.edu [Environmental and Occupational Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ (United States)

    2013-10-15

    The cornea is highly sensitive to oxidative stress, a process that can lead to lipid peroxidation. Ultraviolet light B (UVB) and nitrogen mustard (mechlorethamine) are corneal toxicants known to induce oxidative stress. Using a rabbit air-lifted corneal organ culture model, the oxidative stress responses to these toxicants in the corneal epithelium was characterized. Treatment of the cornea with UVB (0.5 J/cm{sup 2}) or nitrogen mustard (100 nmol) resulted in the generation of 4-hydroxynonenal (4-HNE), a reactive lipid peroxidation end product. This was associated with increased expression of the antioxidant, heme oxygenase-1 (HO-1). In human corneal epithelial cells in culture, addition of 4-HNE or 9-nitrooleic acid, a reactive nitrolipid formed during nitrosative stress, caused a time-dependent induction of HO-1 mRNA and protein; maximal responses were evident after 10 h with 30 μM 4-HNE or 6 h with 10 μM 9-nitrooleic acid. 4-HNE and 9-nitrooleic acid were also found to activate Erk1/2, JNK and p38 MAP kinases, as well as phosphoinositide-3-kinase (PI3)/Akt. Inhibition of p38 blocked 4-HNE- and 9-nitrooleic acid-induced HO-1 expression. Inhibition of Erk1/2, and to a lesser extent, JNK and PI3K/Akt, suppressed only 4-HNE-induced HO-1, while inhibition of JNK and PI3K/Akt, but not Erk1/2, partly reduced 9-nitrooleic acid-induced HO-1. These data indicate that the actions of 4-HNE and 9-nitrooleic acid on corneal epithelial cells are distinct. The sensitivity of corneal epithelial cells to oxidative stress may be an important mechanism mediating tissue injury induced by UVB or nitrogen mustard. - Highlights: • UVB or nitrogen mustard causes rabbit corneal epithelial injury. • 4-Hydroxynonenal (4-HNE) was formed and heme oxygenase-1 (HO-1) was increased. • 4-HNE induced HO-1 mRNA and protein expression in human corneal epithelial cells. • The induction of HO-1 by 4-HNE was through MAP kinase activation.

  13. 大疱性类天疱疮患者临床特征和系统损害与性别的相关性%The Correlation among Gender, Clinical Characteristics and Systemic Damage of Bullous Pemphigoid

    Institute of Scientific and Technical Information of China (English)

    宋蒙蒙; 张敏; 张理涛

    2011-01-01

    目的 评价BP系统损害与性别的相关性.方法 回顾性分析113例BP患者的临床表现、实验室指标和治疗方法.结果 BP患者临床表现与性别不相关(P>0.05);但女性患者血红蛋白量降低的发生率和尿液中白细胞数明显高于男性,男性患者的血尿水平及治疗前后血ALT,BUN和GGT升高的水平均明显高于女性患者(P均<0.05).另外,有肝肾损害者治疗时激素起始用量较无肝肾损害者高,住院时间也较后者长,两者差异均有统计学意义(P均<0.001).结论 BP患者肝肾损害在性别上存在差异,有或无肝肾损害影响患者的治疗和预后,应早期针对肝肾损害情况制定治疗方案,以减少疾病复发.%Objective To compare the clinical characteristics of bullous pemphigoid (BP)in male and female patients correlation between gender and systemic damage of BP. Methods A retrospective analysis was performed on clinical figures, laboratory findings, treatment and the prognosis of the 113 BP patients. Results There was no significant difference between gender in clinical manifestations of BP( P > 0.05 ). In female patients The incidence of lower hemoglobin and urine WBC count in were significantly higher than in male. The level of hematuria and the elevated level of blood ALT, BUN, and GGT before and after treatment in male were significantly higher than that in female (P < 0.05 both). In addition, whether have liver and kidney damage or not, the starting dose of corticosteroid therapy and the time in hospital have significant difference ( P <0.001 both). Conclusion The differences between gender in liver and kidney damage of BP, which impacted the treatment and prognosis is distinct and should be pay attention to, especially in male. The further formulate treatment program should be taken to reduce the recurrence of the disease.

  14. Effects of mitomycin C on infiltration of polymorphonuclear leukocytes after epithelial scrape injury in the mouse cornea Efeito da mitomicina C na infiltração de leucócitos polimorfonucleares após lesão epitelial em córnea de camundongo

    OpenAIRE

    Ana Cecília de Souza Leão Escarião; Takayuki Nagasaki; Jin Zhao; Richard Braunstein

    2008-01-01

    PURPOSE: To determine whether mitomycin C (MMC) alters appearance and disappearance of polymorphonuclear leucocytes (PMN) in the cornea stroma, using an epithelial scrape injury in eye mouse model. METHODS: Twenty-mice underwent mechanical epithelium debridement in the central cornea using 20% ethanol. After the scrape, the right eye received 0.02% MMC for one minute, while the left eye received physiological saline. The animals were sacrificed on days 1, 2, 5, and 14 after surgery, and corne...

  15. Controlled reshaping of the front surface of the cornea through its full-area ablation outside of the optical zone with a Gaussian ArF excimer laser beam

    International Nuclear Information System (INIS)

    We studied in vitro the response of the topography of the cornea to its full-area laser ablation (the laser beam spot diameter is commensurable with the size of the interface) outside of the central zone with an excimer laser having a Gaussian fluence distribution across the beam. Subject to investigation were the topographically controlled surface changes of the anterior cornea in 60 porcine eyes with a 5 ± 1.25-diopter artificially induced astigmatism, the changes being caused by laser ablation of the stromal collagen in two 3.5-mm-dia. circular areas along the weaker astigmatism axis. Experimental relationships are presented between the actual astigmatism correction and the expected correction for the intact optical zones 1, 2, 3, and 4 mm in diameter. The data for each zone were approximated by the least-squares method with the function d = a + bx. The coefficient b is given with the root-mean-square error. The statistical processing of the data yielded the following results: d = (0.14 ± 0.037)x for the 1-mm-dia. optical zone, (1.10 ± 0.036)x for the 2-mm-dia. optical zone, (1.04 ± 0.020)x for the 3-mm-dia. optical zone, and (0.55 ± 0.04)x for the 4-mm-dia. optical zone. Full astigmatism correction was achieved with ablation effected outside of the 3-mm-dia. optical zone. The surface changes of the cornea are shown to be due not only to the removal of the corneal tissue, but also to the biomechanical topographic response of the cornea to its strain caused by the formation of a dense pseudomembrane in the ablation area. (letter)

  16. Controlled reshaping of the front surface of the cornea through its full-area ablation outside of the optical zone with a Gaussian ArF excimer laser beam

    Science.gov (United States)

    Semchishen, A. V.; Semchishen, V. A.

    2014-01-01

    We studied in vitro the response of the topography of the cornea to its full-area laser ablation (the laser beam spot diameter is commensurable with the size of the interface) outside of the central zone with an excimer laser having a Gaussian fluence distribution across the beam. Subject to investigation were the topographically controlled surface changes of the anterior cornea in 60 porcine eyes with a 5 ± 1.25-diopter artificially induced astigmatism, the changes being caused by laser ablation of the stromal collagen in two 3.5-mm-dia. circular areas along the weaker astigmatism axis. Experimental relationships are presented between the actual astigmatism correction and the expected correction for the intact optical zones 1, 2, 3, and 4 mm in diameter. The data for each zone were approximated by the least-squares method with the function d = a + bx. The coefficient b is given with the root-mean-square error. The statistical processing of the data yielded the following results: d = (0.14 ± 0.037)x for the 1-mm-dia. optical zone, (1.10 ± 0.036)x for the 2-mm-dia. optical zone, (1.04 ± 0.020)x for the 3-mm-dia. optical zone, and (0.55 ± 0.04)x for the 4-mm-dia. optical zone. Full astigmatism correction was achieved with ablation effected outside of the 3-mm-dia. optical zone. The surface changes of the cornea are shown to be due not only to the removal of the corneal tissue, but also to the biomechanical topographic response of the cornea to its strain caused by the formation of a dense pseudomembrane in the ablation area.

  17. Genome-Wide Transcriptional Analysis of Differentially Expressed Genes in Diabetic, Healing Corneal Epithelial Cells: Hyperglycemia-Suppressed TGFβ3 Expression Contributes to the Delay of Epithelial Wound Healing in Diabetic Corneas

    OpenAIRE

    Bettahi, Ilham; Sun, Haijing; Gao, Nan; Wang, Feng; Mi, Xiaofan; Chen, Weiping; Liu, Zuguo; Yu, Fu-Shin X.

    2014-01-01

    Patients with diabetes mellitus (DM) may develop corneal complications and delayed wound healing. The aims of this study are to characterize the molecular signatures and biological pathways leading to delayed epithelial wound healing and to delineate the involvement of TGFβ3 therein. Genome-wide cDNA microarray analysis revealed 1,888 differentially expressed genes in the healing epithelia of normal (NL) versus type 1 DM rat corneas. Gene ontology and enrichment analyses indicated TGFβ signal...

  18. A COMPARATIVE STUDY IN AXIAL LENGTH OF EYE AND RADIUS OF CURVATURE OF CORNEA BETWEEN MYOPES AND EMMETROPES IN INDIAN POPULATION

    Directory of Open Access Journals (Sweden)

    Md. Abdul

    2015-06-01

    Full Text Available BACKGROUND: The refractive state of the human eye is dependent on the balance of change in eye size and refractive components, namely, the cornea and crystalline lens. The axial length (AL is the distance from the corneal surfa ce to an interference peak corresponding to the retinal pigment epithelium. Myopia is one of the most common causes of visual impairment worldwide. It is proved in earlier studies that the eye shape is different in myopic and non - myopic children even at a very young age. AIM: The present study was conducted to compare the axial lengths of eye in myopes and emmetropes. MATERIALS & METHODS: Study comprised of Healthy individuals visiting for routine eye check - up and clinically diagnosed Myopia patients visiting outpatient department of Ophthalmology at Vydehi Institute of Medical Sciences and Research Centre, Bangalore. Sample size was 380 . A - Scan Biometry was used to determine the Axial Length of the eye. We compared axial length of eye in myopes & emmetropes. RESULTS: Out of 380 subjects 278 were myopes & 102 were emmetropes. Majority of the subjects (45.6% belong to age group between 21 - 30 years. Axial length was significantly (p<0.05 more in myopes (24.25±0.96 than emmetropes (23.52±0.96 in both the eyes. CONCLUSION: A greater AL of the eye was observed in the ccase group examined. Hence, axial lengthening is the main morphological factor related to myopia.

  19. 人角膜接触镜材料生物相容性的研究%Studies on biocompatibility of contact lens material for human cornea

    Institute of Scientific and Technical Information of China (English)

    朱文渊; 刘正堂; 崔英德; 黎新明

    2007-01-01

    Objective To study the biocompatibility of contact lens material for human cornea prepared in our department. Methods Biological properties of the material were assessed by cytotoxicity,haemolysis and protein aggradation. Results The material had no toxicity for HEFC. The haemolysis rate was eligibility(1.62%). Anti-protein-aggradation was quite good. Conclusion The contact lens material by our department possesses quite good biological properties.%目的 研究本室制备的人角膜接触镜材料的生物性能.方法 采用细胞毒性试验、红细胞溶血试验以及蛋白质沉积试验方法.结果 所制备的人角膜接触镜材料对人胚肺纤维细胞(HEFC)毒性评价为1级无毒性;红细胞溶血率为1.62%合格;在体温范围内抗蛋白质沉积作用较好.结论 本室制备的人角膜接触镜材料具有较好的生物相容性.

  20. Comparison of effect of nepafenac and diclofenac ophthalmic solutions on cornea, tear film, and ocular surface after cataract surgery: the results of a randomized trial

    Science.gov (United States)

    Kawahara, Atsushi; Utsunomiya, Tsugiaki; Kato, Yuji; Takayanagi, Yoshinori

    2016-01-01

    Background The aim of this study was to compare the effects of nepafenac ophthalmic suspension 0.1% (Nevanac) and diclofenac sodium ophthalmic solution 0.1% (Diclod) on the cornea, tear film, and ocular surface after cataract surgery. Methods A total of 60 eyes (60 patients) were selected for this study, with no ocular diseases other than cataract (scheduled for cataract surgery by one surgeon). Patients were randomly enrolled to receive nepafenac or diclofenac in the perioperative period, and cataract surgery was performed using torsional microcoaxial phacoemulsification and aspiration with intraocular lens implantation via a transconjunctival single-plane sclerocorneal incision at the 12 o’clock position. We compared intra- and intergroup differences preoperatively and postoperatively in conjunctival and corneal fluorescein staining scores, tear film breakup times, Schirmer’s tests, the Dry Eye Related Quality of Life Scores, and tear meniscus areas using anterior segment optical coherence tomography. Results The diclofenac group had significantly higher conjunctival and corneal fluorescein staining scores at 4 weeks postoperatively compared with the nepafenac group (Pcataract surgery. PMID:27019091

  1. The Changes of TGF-α, TGF-β1 and Basic FGF Messenger RNA Expression in Rabbit Cornea after Photorefractive Keratectomy

    Institute of Scientific and Technical Information of China (English)

    Yisheng Zhong; Yingrning Zhou; Jingcai Lian; Wen Ye; Kangsun Wang; Feng Cheng

    2000-01-01

    Objective: To study the mechanism of haze formation and investigate the expression changes of transforming growth factor-αα(TGF-αα), transforming growth factor-β1 (TGF-β1)and basic fibroblast growth factor (bFGF) mRNA in corneal epithelium and stroma after photorefractive keratectomy (PRK).Methods: Sixteen white rabbits were randomly divided into 4 groups, and PRK was performed on each eye of 12 rabbits. The haze formation was examined under a slit-lamp microscope at the 1st, 2na and 3ra month after PRK, and the expressions of TGF-αα , TGF-βi and bFGF mRNA were detected with in situ hybridization.Results: The corneal haze formed at the 1st month after PRK. The most prominent haze formation was observed at the 2nd month, and declined gradually at the 3ra month after ablation. TGF-αα mRNA expression was presented on the normal corneal epithelium and not on the corneal stroma. TGF-βl and bGFG mRNA were expressed by both corneal epitheliurn and stroma. The capacities for cornea tissue expression of three growth factors mRNA increased after PRK, and the peaks appeared on the 1s, 2na month. The extent for expressions of three growth factors related proportionally to the haze formation.Conclusion: Three growth factors took part in promoting corneal wound healing after PRK, and might contribute to corneal haze formation and development.

  2. Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model.

    Science.gov (United States)

    Kaluzhny, Yulia; Kandárová, Helena; d'Argembeau-Thornton, Laurence; Kearney, Paul; Klausner, Mitchell

    2015-01-01

    To comply with the Seventh Amendment to the EU Cosmetics Directive and EU REACH legislation, validated non-animal alternative methods for reliable and accurate assessment of ocular toxicity in man are needed. To address this need, we have developed an eye irritation test (EIT) which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model that is based on normal human cells. The EIT is able to separate ocular irritants and corrosives (GHS Categories 1 and 2 combined) and those that do not require labeling (GHS No Category). The test utilizes two separate protocols, one designed for liquid chemicals and a second, similar protocol for solid test articles. The EIT prediction model uses a single exposure period (30 min for liquids, 6 hr for solids) and a single tissue viability cut-off (60.0% as determined by the MTT assay). Based on the results for 83 chemicals (44 liquids and 39 solids) EIT achieved 95.5/68.2/ and 81.8% sensitivity/specificity and accuracy (SS&A) for liquids, 100.0/68.4/ and 84.6% SS&A for solids, and 97.6/68.3/ and 83.1% for overall SS&A. The EIT will contribute significantly to classifying the ocular irritation potential of a wide range of liquid and solid chemicals without the use of animals to meet regulatory testing requirements. The EpiOcular EIT method was implemented in 2015 into the OECD Test Guidelines as TG 492. PMID:26325674

  3. Normal X-inactivation mosaicism in corneas of heterozygous FlnaDilp2/+ female mice--a model of human Filamin A (FLNA diseases

    Directory of Open Access Journals (Sweden)

    Douvaras Panagiotis

    2012-02-01

    Full Text Available Abstract Background Some abnormalities of mouse corneal epithelial maintenance can be identified by the atypical mosaic patterns they produce in X-chromosome inactivation mosaics and chimeras. Human FLNA/+ females, heterozygous for X-linked, filamin A gene (FLNA mutations, display a range of disorders and X-inactivation mosaicism is sometimes quantitatively unbalanced. FlnaDilp2/+ mice, heterozygous for an X-linked filamin A (Flna nonsense mutation have variable eye, skeletal and other abnormalities, but X-inactivation mosaicism has not been investigated. The aim of this study was to determine whether X-inactivation mosaicism in the corneal epithelia of FlnaDilp2/+ mice was affected in any way that might predict abnormal corneal epithelial maintenance. Results X-chromosome inactivation mosaicism was studied in the corneal epithelium and a control tissue (liver of FlnaDilp2/+ and wild-type (WT female X-inactivation mosaics, hemizygous for the X-linked, LacZ reporter H253 transgene, using β-galactosidase histochemical staining. The corneal epithelia of FlnaDilp2/+ and WT X-inactivation mosaics showed similar radial, striped patterns, implying epithelial cell movement was not disrupted in FlnaDilp2/+ corneas. Corrected stripe numbers declined with age overall (but not significantly for either genotype individually, consistent with previous reports suggesting an age-related reduction in stem cell function. Corrected stripe numbers were not reduced in FlnaDilp2/+ compared with WT X-inactivation mosaics and mosaicism was not significantly more unbalanced in the corneal epithelia or livers of FlnaDilp2/+ than wild-type Flna+/+ X-inactivation mosaics. Conclusions Mosaic analysis identified no major effect of the mouse FlnaDilp2 mutation on corneal epithelial maintenance or the balance of X-inactivation mosaicism in the corneal epithelium or liver.

  4. Process for producing a collagen web for a transparent artificial cornea and a contact lens by irradiation with ultraviolet or gamma rays

    International Nuclear Information System (INIS)

    A process for producing a collagen web for use as an artificial cornea and a contact lens is provided by irradiating an acid soluble collagen web or a swelled collagen web with ultraviolet rays or gamma radiation. Cross-linking is introduced into the molecules forming a noncrystalline portion of the collagen to minimize nonuniformity of construction. In embodiments, the collagen, dissolved in a diluted hydrochloric acid or acetic acid aqueous solution at a concentration of about 0.5% to 1%(pH 2-4), is poured into a suitable container for producing and drying a web. The obtained web is neutralized with ammoniac water or vapor and is washed completely. The web is dipped in a dilute hydrochloric acid or acetic acid aqueous solution (pH 2-4) to well and then irradiated. Cross-linking is introduced, followed by further neutralization with ammoniac vapor. Finally the web is washed, air-dried or kept in water. Alternately, the above 0.5%-1% collagen solution is irradiated to gelatinize it after the oxygen dissolved in the solution is substituted with nitrogen. The irradiated web is neutralized with ammoniac water or vapor, and then washed, air-dried or kept in water. In one example, 0.58% collagen hydrochloric acid solution was poured into a methylmethacrylate box and was air-dried to form a web 0.03mm in thickness. The web was neutralized with ammoniac vapor for 24 hours and dipped to swell in a 0.01N HCl solution for 1 minute. The opposite sides of the web were irradiated with UV-rays at 15W. (Iwakiri, K.)

  5. Medical image of the week: bullous emphysema

    OpenAIRE

    Tey KR; Ajayi-Obe A; Mahmoud N

    2016-01-01

    No abstract available. Article truncated at 150 words. A 63-year-old gentleman, with a history of 90-pack-years of smoking and stage IV chronic obstructive pulmonary disease was receiving home oxygen at 2 L/min at baseline. He has had multiple prior hospital admissions for respiratory failure. Over the past 2 weeks he has had increased production of sputum, associated with worsening shortness of breath. He is on fluticasone-salmeterol inhaler, albuterol inhaler, and tiotropium as an outpatien...

  6. Motivos do descarte de córneas captadas pelo banco de olhos do Hospital São Paulo em dois anos Discarding causes of cornea collected by eye bank of São Paulo hospital in two years

    Directory of Open Access Journals (Sweden)

    Carla Galluzzi dos Santos

    2010-02-01

    Full Text Available OBJETIVO: Analisar os motivos do descarte ou não utilização de córneas doadas, cujos tecidos foram captados, preservados e avaliados pelo Banco de Olhos do Hospital São Paulo no período de outubro de 2002 a setembro de 2004. MÉTODOS: Estudo retrospectivo analisando os prontuários com as seguintes informações sobre as doações: história clínica e ocular do doador, idade, causa mortis, resultados de exames sorológicos, avaliação do corpo do doador, avaliação biomicroscópica da morfologia do globo ocular e da córnea e contagem celular endotelial. RESULTADOS: De 1.116 córneas doadas no período, 518 (46,41% foram descartadas antes de serem preservadas, sendo 288 (25,80% devido à causa mortis, 56 (5,01% pelo histórico clínico do doador e 174 (15,59% pela avaliação do tecido. Das 598 córneas preservadas, 317 (28,40% foram destinadas ao transplante óptico e 168 (15,05% indicadas para transplantes tectônicos. CONCLUSÃO: A análise do prontuário hospitalar e história clínica do doador foi um importante fator de descarte de córneas antes da preservação. Já a avaliação biomicroscópica realizada na lâmpada de fenda é uma etapa fundamental para qualificação e classificação do tecido adequando-o para o paciente e garantindo um bom resultado cirúrgico.PURPOSE: To analyze causes of discarded donor corneas which were collected and preserved by São Paulo Hospital Eye Bank in a period of two years. METHODS: Retrospective study based in the investigation of donor's medical records, for clinical and ocular factors, age, death cause, serology results, body conditions, slit lamp corneal examination and endothelial cells counting. RESULTS: 1,116 corneas were donated and included in the study, 518 (46.41% were discarded before preservation, 168 (15.05% were not transplanted for optical purpose, but used for tectonic indication. The main reason of cornea discard was the cause of death in 288 (25.80% corneas, second the slit

  7. Nerve fibers that were not stained with the non-specific acetylcholinesterase (NsAchE) method, and TRPV1- and IB4-positive nerve fibers in the rat cornea.

    Science.gov (United States)

    Nakagawa, Hiroshi; Hiura, Akio; Mitome, Masato; Ishimura, Kazunori

    2009-08-01

    Previously, we noticed the presence of nerve fiber-like structures in a whole mount preparation of the rat cornea that had not been stained with the non-specific acetylcholinesterase (NsAchE) method. These nerve-like fibers were projected into the central area of the cornea, forming a mesh-like pattern. The aim of this study is to examine the properties of these mesh-like fibers using the following two methods: their sensitivity to capsaicin and the detection of isolectin B4 (IB4)- and capsaicin receptor TRPV1 (transient receptor potential vanilloid 1)-reactivities. The mean disappeared area of non-stained fibers after NsAchE treatment was 26% of the total areas in the neonatally capsaicin-treated cornea. Bunches composed of fine IB4-positive nerve fibers were seen in a whole mount preparation. There were connections between the bunches, producing a mesh-like pattern similar to that of the fibers that were not stained with NsAchE. Fine TRPV1-immunoreactive (ir) nerve fibers were also shown to form bunches, with connections between each bunch observed in whole mount preparations. Thus, TRPV1-ir nerve fibers seem to densely innervate the rat corneal subepithelial stroma and are distinct from the NsAchE-positive nerve fibers. The TRPV1-ir fine nerve fibers overlapped with the IB4-positive nerve fibers, suggesting that the mesh-like fibers that were not stained with NsAchE are fine nociceptive sensory nerve fibers because of their sensitivity to capsaicin and similar distribution pattern to IB4- and TRPV1-positive nerve fibers. PMID:19763029

  8. Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study

    Directory of Open Access Journals (Sweden)

    Sindt CW

    2012-03-01

    Full Text Available Christine W Sindt1, Trudy K Grout1, D Brice Critser1, Jami R Kern2, David L Meadows21University of Iowa Hospitals and Clinics, Iowa City, IA; 2Alcon Research Ltd, Fort Worth, TX, USABackground: The purpose of this study was to assess whether differences in central corneal dendritic immune cell densities associated with combinations of soft contact lenses and lens care solutions could be detected by in vivo confocal microscopy.Methods: Participants were adults naïve to contact lens wear (n = 10 or who wore soft contact lenses habitually on a daily-wear schedule (n = 38 or on a study-assigned schedule for 30 days with daily disposable silicone hydrogel lenses (n = 15. Central corneas were scanned using an in vivo confocal microscope. Cell densities were compared among groups by demographic parameters, lens materials, and lens care solutions (polyhexamethylene biguanide [PHMB], polyquaternium-1 and myristamidopropyl dimethylamine [PQ/MAPD], peroxide, or blister pack solution [for daily disposable lenses].Results: Among lens wearers, no associations were observed between immune cell densities and age, gender, or years of lens-wearing experience. Mean cell density was significantly lower (P < 0.01 in nonwearers (29 ± 23 cells/mm2, n = 10 than in lens wearers (64 ± 71 cells/mm2, n = 53. Mean cell density was lower (P = 0.21 with traditional polymer lenses (47 ± 44 cells/mm2, n = 12 than with silicone hydrogel lenses (69 ± 77 cells/mm2, n = 41. Lowest to highest mean density of immune cells among lens wearers was as follows: PQ/MAPD solution (49 ± 28 cells/mm2, blister pack solution (63 ± 81 cells/mm2, PHMB solution (66 ± 44 cells/mm2, and peroxide solution (85 ± 112 cells/mm2.Conclusion: In this pilot study, in vivo confocal microscopy was useful for detecting an elevated immune response associated with soft contact lenses, and for identifying lens-related and solution-related immune responses that merit further research.Keywords: Clear Care

  9. The cornea in systemic disease

    OpenAIRE

    W. D. H. Gillan

    2005-01-01

    Systemic  disease  often  results  in  corneal signs that are easily observable by means of bio-microscopy. Optometry is in a unique position to detect the corneal manifestations of systemic disease. This review presents corneal signs of various systemic diseases. The list is not com-plete but the review rather attempts to empha-size the importance of detection, and referral, of systemic diseases.

  10. Facoemulsificação por córnea clara no meridiano mais curvo Phacoemulsification using clear cornea incision in steepest meridian

    Directory of Open Access Journals (Sweden)

    Fernando Pistarini Gonçalves

    2007-03-01

    Full Text Available OBJETIVOS: Avaliar o efeito da facoemulsificação com incisão em córnea clara no meridiano mais curvo sobre a magnitude do astigmatismo ceratométrico pré-operatório. Mapear a magnitude do astigmatismo cirurgicamente induzido por incisões nas posições nasal (N, temporal (T, temporal superior (TS e temporal inferior (TI. MÉTODOS: Foi realizado estudo ceratométrico prospectivo em 48 olhos de 48 pacientes, submetidos a facoemulsificação com incisão do meridiano mais curvo. As medidas ceratométricas foram realizadas no pré-operatório e um mês após a realização da cirurgia. O astigmatismo cirurgicamente induzido foi determinado pelo método das coordenadas retangulares em 10 passos, modificado. RESULTADOS: Foram operados 21 olhos direitos e 27 olhos esquerdos e, de acordo com a posição das incisões em córnea clara, foram divididos em: 16 olhos N, 4 olhos T, 22 olhos TS, 6 olhos TI. A média da ametropia cilíndrica pré-operatória foi de 1,06D ± 0,65 e a pós-operatória de 0,89D ± 0,80. Houve diminuição estatisticamente significativa no astigmatismo corneal preexistente (p=0,016. A média total de astigmatismo cirurgicamente induzido foi de 0,94D ± 0,56. Em relação aos grupos o astigmatismo cirurgicamente induzido foi de 1,06D ± 0,66 em N, 0,87D ± 0,20 em T, 0,95D ± 0,55 em TS e 0,61D ± 0,25 em TI. Não houve diferença estatística com relação ao astigmatismo cirurgicamente induzido nos grupos N, T, TS e TI (p=0,426. CONCLUSÃO: A técnica se mostrou efetiva na redução do astigmatismo ceratométrico pré-operatório. A média do astigmatismo cirurgicamente induzido foi de 1,06D ± 0,66 em N, 0,95D ± 0,55 em TS, 0,61D ± 0,25 em T e 0,87D ± 0,20 em TI.PURPOSE: To evaluate the effect of phacoemulsification through clear cornea incision in the steepest meridian (ISM, on the magnitude of preoperative keratometric astigmatism. To map the magnitude of surgically induced astigmatism (SIA by clear cornea incision

  11. 多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用%Doxycycline inhibits the infiltration of inflammatory cells in rat alkali burn corneas

    Institute of Scientific and Technical Information of China (English)

    赵静博; 邹文进; 付馨余

    2013-01-01

    AIM: To investigate the inhibited effect of Doxycycline on the infiltration of inflammatory cells in rat alkali burn corneas.METHODS: Corneas of 32 SD rats were injured with 1 mol/L NaOH, then divided into two groups: control and doxycycline - treated. All agents were administered topically 4 times daily. Slit - lamp microscope was performed and inflammatory index was calculated at 3, 7, 14 and 21 days after injury. Then 4 rats were randomly sacrificed and each cornea was divided into two parts, one for histopathology, the other for ICAM - 1 ELISA assay.RESULTS:ln control group, the inflammatory index and the number of inflammatory cell was higher than the doxycycline-treated dramatically at all time points ( P< 0.05). Compared with control group, corneal ICAM - 1 expression decreased significantly in doxycycline-treated group at all time points( P < 0.05).CONCLUSION: Doxycycline may inhibit inflammatory cell infiltration by down-regulating ICAM-1 expression.%目的:观察多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用.方法:健康SD大鼠32只,随机分为对照组、多西环素组,每组16只大鼠.建立角膜碱烧伤模型后,多西环素组予3g/L多西环素眼液点眼,对照组予眼液溶媒点眼.分别于碱烧伤后第3,7,14,21d观察计算炎症指数,角膜取材后进行病理切片及炎症细胞计数,行ICAM-1的ELISA检测.结果:多西环素组各时间点结膜充血、角膜水肿较对照组轻,炎症指数均明显低于对照组,差异有统计学意义(P< 0.05).多西环素组各时间点炎症细胞计数少于对照组(P< 0.05).多西环素组各时间点大鼠角膜的ICAM-1表达低于对照组(P<0.05).结论:多西环素可以抑制碱烧伤大鼠角膜的炎症细胞浸润,其机制可能是抑制ICAM-1的表达.

  12. Distribution of trace elements in the mammalian retina and cornea by use of particle-induced X-ray emission (PIXE): localisation of zinc does not correlate with that of metallothioneins.

    Science.gov (United States)

    Ugarte, Marta; Grime, Geoffrey W; Osborne, Neville N

    2014-02-01

    Proton-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) was used to establish the distribution and concentration of trace elements within individual corneal and retinal areas in frozen sections from adult male Wistar rats (n = 6). The distribution of endogenous trace elements in the cornea and retina is non-homogenous. The most abundant metal in the cornea is calcium followed by zinc. Iron and copper are present in small amounts localised particularly to the epithelium. Iron is also identified in keratocytes. Relatively high levels of calcium occur in the corneal epithelial cell bodies. Zinc has a wide intense distribution across the corneal epithelium (with greater levels in the basal part) and posterior stroma. In the retina, zinc is the most common metal followed by iron and copper. Relatively high levels of zinc exist in the retinal pigment epithelium (RPE), photoreceptor inner segments (RIS) and inner nuclear layer (INL). Chelatable zinc was localised with fluorescent TSQ in the RPE, RIS and plexiform layers. It is interesting to note that the highest levels of total zinc and the greatest intensity of chelatable zinc staining do not coincide. In the RPE and corneal epithelium, zinc co-localised with the zinc-containing metallothioneins (MT). However, there was a clear mismatch between the localisation of the most intense levels of zinc in the neuroretina (i.e. INL) and corneal posterior stroma with that reported for MT. For example, the presence of zinc is not particularly associated with the retinal ganglion cells, retinal area that contains MTs in significant amounts. While high amounts of zinc are present in the INL and corneal posterior stroma, which are largely devoid of MTs. This probably represents pools of static, catalytic and structural zinc associated with substances other than the MTs. PMID:24226809

  13. Color light-emitting diode reflection topography: validation of keratometric repeatability in a large sample of wide cylindrical-range corneas

    Directory of Open Access Journals (Sweden)

    Kanellopoulos AJ

    2015-02-01

    (0.02 to 12.13° for the keratoconic Group B, and 3.20±1.99° (0.17 to 8.61° for the cross-linked Group C. The control Group D had axis repeatability 2.16±1.39° (0.05 to 5.86°. The SAI index measurement repeatability was 0.33±0.40 (0.01 to 2.31 in the post-LASIK Group A, 0.39±0.75 (0.00 to 7.15 in the keratoconic Group B, and 0.43±0.56 (0.05 to 3.50 in the keratoconus post-CXL Group C. The control group had SAI measurement repeatability of 0.26±0.30 (0.00 to 2.39. The SRI index repeatability was 0.22±0.17 (0.01 to 0.96 for post-LASIK Group A, 0.20±0.18 (0.00 to 1.07 in keratoconic Group B, and 0.13±0.09 (0.00 to 0.45 in the keratoconus post-CXL Group C. The control Group D had SRI measurement repeatability of 0.23±0.16 (0.00 to 0.75.Conclusion: This novel corneal topography device appears to offer very high specificity in estimating corneal keratometry and specific corneal irregularity indices, even in topographically challenging corneas such as LASIK treated, keratoconic, and cross-linked.Keywords: LED topography, point-source topography, VU topographer, axis of astigmatism, Cassini topographer, steep meridian, surface regularity index, surface asymmetry index, form factor

  14. Effects of Diabetes on the Eye

    OpenAIRE

    Lutty, Gerard A.

    2013-01-01

    Hyperglycemia has toxic effects on almost all cells in the body. Ophthalmic complications of hyperglycemia are most profound in cornea and retina. Seventy percent of diabetics suffer from corneal complications, collectively called diabetic keratopathy, which includes include recurrent erosions, delayed wound healing, ulcers, and edema. Confocal microscopy has permitted in vivo imaging of corneal nerves, which are also affected in diabetic subjects. Gene therapies upregulating MNNG HOS transfo...

  15. Application of whole conjunctival fascia flap coveting cornea in plasty of trophic eyeball%结膜筋膜瓣覆盖角膜术在眼球萎缩整形中应用

    Institute of Scientific and Technical Information of China (English)

    任彦新; 马景学; 卫玉彩; 耿玉欣; 包永琴

    2014-01-01

    Objective To discuss a method and clinical effect of wearing thin ocular prosthesis after whole conjunctiva fascia flap covering operation.Methods A retrospective study.A total of 18 cases with mild-to-moderate eyeball atrophy compared exophthalmos with the other healthy eye that differed from 4 to 7 mm.According to the medical order,18 patients were divided into groups A and B.In the operation,the corneal epithelium and limbs epithelial tissue of atrophy eyeball were thoroughly removed,in which 10 cases (group A) the above the bulbar conjunctiva and fascia were slid downward,to cover the whole cornea and were fixed in the sclera below the limbs,and stratified suture was used in the below fascia and conjunctiva.Eight cases (group B),the above fascia conjunctiva and beneath fascia conjunctiva were stratified sutured and fixed in corneal central part.After a month,customized personalized ocular prosthesis was used,followed-up of 3 months to two years,to observe the curative effect.Results The conjunctiva fascia coverage of 10 patients in group A was good,with no corneal exposure and foreign body sensation,8 patients in group B,in which there was a horizontal crack in central cornea in two patients respectively,with foreign body sensation.The bilateral palpebral fissure height,orbital area plumpness and eye activity were similar in two groups,and the improved outward appearance was satisfactory,which reached the cosmetic effect Conclusions By apply complete conjunctiva fascia flap to cover cornea in mild-to-moderate eyeball atrophy,the healing of postoperative fascia and cornea is good,and the cornea is not easy exposed.With personalized ocular prosthesis,the cosmetic effect is good.%目的 探讨保留萎缩眼球配戴薄型义眼的方法和临床效果.方法 回顾性研究对2010年2月至2012年8月在河北医科大学第二医院眼科就诊的轻中度眼球萎缩患者18例(同健眼相比眼球突出度相差4~7 mm),按照就诊顺序分成A组(10

  16. MHC-Ⅱ类抗原在角膜碱烧伤后眼组织的动态改变%MHC class Ⅱ positive cells in the eye tissue of rats after alkali burn of cornea

    Institute of Scientific and Technical Information of China (English)

    赵敏; 陈家祺; 杨培增

    2001-01-01

    目的:探讨角膜碱烧伤后角膜溃烂溶解穿孔以及眼内炎症的免疫学机制。方法:在大鼠角膜上制作碱烧伤模型,在烧伤后的不同阶段,制备角膜、虹膜、脉络膜以及视网膜铺片,采用卵白素-生物素过氧化物酶免疫组化方法(ABC法),检测眼组织MHC-Ⅱ类抗原阳性细胞。结果:烧伤初期,角膜及虹膜即有MHC-Ⅱ类抗原阳性细胞的轻度增多;在角膜溶解穿孔阶段,MHC-Ⅱ类抗原阳性细胞大量出现,密集分布于角膜缘及溃疡溶解处,以及虹膜、脉络膜组织中,且在形态学上也发生了一些变化。当烧伤后期溃疡愈合角膜病变稳定时,虹膜上依然见较多阳性细胞。正常视网膜细胞未见MHC-Ⅱ类抗原的表达,但在烧伤后角膜溶解期以及稳定期,均可见Ⅱ类抗原阳性细胞。结论:免疫反应可能参与了严重角膜碱烧伤角膜溶解及眼内炎症的病理过程,其发生机理可能与自身免疫反应有关。%Objective: To investigate immunological mechanism of corneal melting, perforation and ophthalmitis after alkali burn. Methods: A model of severe corneal alkali burn was established in rats. Immunohistochemical method (ABC method) was carried out on wholemounts of the cornea, iris, choroid and retina at different time after corneal burns to detect MHC class Ⅱ positive cells. Results: In the early stage of the burn, slight increase of the number of MHC class Ⅱ positive cells in the cornea and iris was found. At the time of corneal melting and perforation, a massive influx of the positive cells was not only seen at the limbus but also in the corneal melting area, iris and choroid. Morphological changes of the cells were also noted. In restoration stage, a number of positive cells was also found in iris. In the normal control group and in the early stage of the burn, the MHC class Ⅱ antigen was not expressed in the retina tissue, but at the the time of corneal

  17. The effect of LASIK with Kappa angle adjust on aberration in human cornea%LASIK 术中 Kappa 角调整对角膜像差影响的研究

    Institute of Scientific and Technical Information of China (English)

    周春阳; 陈婕; 谢艾芮; 何清; 胡缘

    2013-01-01

    Objective To investigate the aberration changes of human cornea after LASIK with angle Kappa adjusted in myopic eyes compared with conventional LASIK .Methods LASIK with angle Kappa adjust was performed in 30 cases (60 myopic eyes) ,and conventional LASIK was performed in 30 cases (60 myopic eyes) .All the data of patients were evaluated by the ALLEGRO TOPOLYZER at 3 months after the surgery .Results Value of C3 ± 1 and C3 ± 3 was lower in LASIK with angle Kappa adjust group than that in LASIK with conventional group(P0 .05) .Conclusion LASIK with Kappa angle adjust can cut down the aberration of C3 ± 1、and C3 ± 3 in human cornea compared with conventional LASIK .%目的:通过对比kappa角调整 LASIK术与标准LASIK术术后角膜高阶像差的变化,探讨Kappa角调整对LASIK术后高阶像差的影响。方法近视LASIK手术患者,分为Kappa角调整 LASIK组、标准LASIK组两组,每组各30例(60眼),以ALLEGRO TOPOLYZER角膜地形图仪,对比两组术后3月时角膜高阶像差的差异。结果 Kappa角调整LASIK组较之于标准LASIK组,彗差C3±1、三叶草C3±3两种高阶像差均明显偏低( P<0.01),而球差C40无明显差异( P>0.05)。结论 Kappa角调整的LASIK术较之于标准LASIK术,能减少角膜由切削偏中心引起的彗差C3±1、三叶草C3±3两种高阶像差的产生。

  18. Avaliação da qualidade das córneas doadoras em relação à idade do doador e causa do óbito Evaluation of the quality of donor corneas in relation to the age of donor and cause of death

    Directory of Open Access Journals (Sweden)

    Gleisson Rezende Pantaleão

    2009-10-01

    Full Text Available OBJETIVO: Analisar a qualidade das córneas avaliadas em lâmpada de fenda no Banco de Tecidos Oculares do Hospital de Clínicas da Universidade Federal do Paraná (BTO HC-UFPR relacionando com a idade e causa de óbito. MÉTODOS: Foram analisadas retrospectivamente fichas de avaliações padronizadas de 492 córneas, avaliadas no BTO HC-UFPR, do período de agosto de 2006 a agosto de 2008. Cada córnea avaliada foi classificada em relação a sua qualidade em: Muito bom, bom, regular e ruim; mediante os seguintes fatores: claridade da córnea, edema epitelial, defeito epitelial, edema estromal, dobras de Descemet, opacidade estromal, cicatrizes corneanas, densidade endotelial e guttata. RESULTADOS: A idade média dos doadores foi de 42,74 anos (dp=17,77. Dentre as causas de óbito, a mais comum foi trauma com 46,18%, seguindo por causas cardiovasculares com 41,86%. Na avaliação da córnea, foram classificadas: 57,11% como "bom", seguido por 20,73% "regular", 16,87% "muito bom" e 5,28% "ruim". Com aplicação de testes estatísticos baseados em modelo de regressão ordinal verificou-se que as córneas provenientes de mortes por traumas tendem a ser melhores, bem como aquelas de pacientes mais jovens. CONCLUSÕES: Estatisticamente, córneas de doadores mais jovens tendem a ter melhores graduações na avaliação, bem como córneas provenientes de doadores com morte por trauma tendem a ser de melhor qualidade quando comparadas com as provenientes de mortes por doenças cardiovasculares e outras doenças sistêmicas.PURPOSE: To analyze the quality of the corneas evaluated by slit lamp examination in the Eye Bank of the Hospital de Clínicas UFPR and its relation to donor age and cause of death. METHODS: Analysis of 492 corneas, evaluated in BTO HC-UFPR between August 2006 to August 2008. Each cornea was classified regarding the quality as: very good, good, regular and bad; and according to clarity, epithelial defect, stromal edema, Descemet

  19. Descarte de córneas por sorologia positiva do doador no Banco de Olhos do Hospital São Paulo: dois anos de estudo Discarded corneas due to positive donor's serologic test in the Hospital São Paulo Eye Bank: a two-year study

    Directory of Open Access Journals (Sweden)

    Marco Túlio Chater Viegas

    2009-04-01

    Full Text Available OBJETIVOS: Investigar descarte de córneas por sorologia positiva de doadores no Banco de Olhos do Hospital São Paulo (BOHSP durante período de dois anos. MÉTODOS: Revisão retrospectiva de prontuários de doadores de córneas do BOHSP entre janeiro de 2006 a dezembro de 2007. Correlações de dados como sorologias dos doadores (Hepatites B, C e HIV, procedência, sexo e faixa etária dos mesmos foram testados. RESULTADOS: Das 902 córneas processadas no BOHSP, 12,9% (116 apresentaram resultado positivo para hepatite B, hepatite C ou HIV em seus doadores; 20,5% (185 apresentaram testes sorológicos inconclusivos perfazendo uma porcentagem de 33,4% (301 de córneas descartadas no período estudado devido a resultado positivo ou inconclusivo da sorologia dos doadores. CONCLUSÃO: O estudo confirma a validade e a importância da realização de testes sorológicos para prevenção de doenças que poderiam ser transmitidas aos eventuais receptores de córneas. Entretanto, testes mais eficazes são necessários para diminuir o número de casos de sorologias inconclusivas, visando diminuir o descarte de córneas que poderiam ser utilizadas.PURPOSE: To investigate discarded corneas due to positive serologic tests in donors from the Hospital São Paulo Eye Bank (BOHSP during a two-year period. METHODS: Retrospective study of records from cornea donors between January 2006 and December 2007. Information such as serologic test results (Hepatitis B, C, and HIV, source of corneal tissue, donor's gender and age were tested for correlation. RESULTS: 902 corneas were processed by BOHSP; 12.9% (116 were discarded due to donor's positive test for hepatitis B, C, or HIV; 20.5% (185 were also discarded due to inconclusive result of serological tests; percentage of corneas discarded due to positive or inconclusive serological tests during this period was 33.4% (301. CONCLUSION: This study confirms the importance of serological tests in order to prevent disease

  20. Efeito do uso de lente escleral cosmética na sensibilidade tátil corneal em phthisis bulbi Effect of ocular prosthesis on cornea sensitivity in phthisis bulbi

    Directory of Open Access Journals (Sweden)

    Lucia Miriam Dumont Lucci

    2004-10-01

    Full Text Available OBJETIVOS: Comparar a sensibilidade tátil corneal entre olhos normais e aqueles com phthisis bulbi e avaliar as alterações provocadas na sensibilidade tátil corneal pelo uso de lente escleral cosmética em phthisis bulbi. MÉTODOS: Estudo prospectivo de 23 pacientes com phthisis bulbi unilateral. Foram realizadas medidas da sensibilidade tátil corneal em ambos os olhos utilizando-se o estesiômetro de Cochet-Bonnet antes e após 3 meses do uso da lente escleral cosmética. RESULTADOS: Em todos os pacientes, a medida da sensibilidade tátil corneal no olho com phthisis bulbi foi menor do que no contralateral (controle. Em 96% houve diminuição da sensibilidade tátil corneal após a adaptação da lente escleral cosmética. CONCLUSÃO: Após a adaptação da lente escleral cosmética, ocorre diminuição da sensibilidade tátil corneal nos olhos. A fisiopatologia é semelhante à encontrada nos usuários de lentes de contato.PURPOSE: To compare corneal sensitivity between normal eyes and those whith phthisis bulbi and also to analyze the alterations of corneal sensitivity in phthisis bulbi induced by wearing ocular prosthesis. METHODS: Prospective study of 23 patients with unilateral phthisis bulbi. Bilateral cornea sensitivity was evaluated using the Cochet-Bonnet esthesiometer before and after 3 months of wearing ocular prosthesis. RESULTS: In all patients, corneal sensitivity of the eye with phthisis bulbi was lower than that of the normal eye (control. In 96% there was decrease of corneal sensitivity after adaptation of ocular prosthesis. CONCLUSION: After wearing ocular prosthesis, there is a reduction in corneal sensitivity in phthisis bulbi. The pathophysiology seems to be the same as that found in contact lens wearers.

  1. Interferón alfa-2b tópico como primera opción en las neoplasias intraepiteliales corneoconjuntivales Topical interferon alfa-2b for primary treatment of conjunctiva-cornea intraepithelial neoplasia

    Directory of Open Access Journals (Sweden)

    M. Pérez de Arcelus

    2012-04-01

    Full Text Available Se describen dos casos de neoplasia intraepitlelial corneo-conjuntival (CIN tratados con interferón alfa-2b (IFN alfa-2b tópico como primera elección. El tratamiento clásico de los CIN ha sido tradicionalmente la resección completa con márgenes de seguridad seguida de crioterapia en el lecho quirúrgico. No obstante, y puesto que la tasa de recidivas puede alcanzar el 50% han sido propuestos coadyuvantes como la mitomicina C y el 5 fluoracilo, con el consiguiente riesgo de toxicidad corneal y límbica. El IFN alfa-2b presenta una eficacia similar a la cirugía en la erradicación completa de la masa tumoral como primera opción, con escasos efectos secundarios y nulo potencial carcinogénico, incluso en casos de recurrencia a terapia con mitomicina C, lesiones quirúrgicas residuales y formas difusas.We describe two cases of conjunctival-cornea intraepithelial neoplasia (CIN, treated with topical IFN alfa 2b. The traditional treatment for CIN is surgical excision usually with adjunctive cryotherapy. However, residual tumour may remain, which can lead to recurrence rates of more than 50%. 5-Fluorouracil, mitomicyn C and interferon alfa 2b are new pharmacological agents that have proved their efficacy in the treatment of CIN. As side effects are common, we present IFN alfa 2b as a single therapeutic agent as an effective and optimal treatment for presumed recurrent corneal and conjunctival intraepithelial neoplasia. It offers the benefits of topical therapy and avoids the risks of surgical or other interventions - specifically, ocular surface toxicity, cicatricial conjunctival changes, and limbal stem cell deficiency.

  2. Corneal Transplant Infection due to Alternaria alternata: A Case Report

    Directory of Open Access Journals (Sweden)

    Vasileios Konidaris

    2013-01-01

    Full Text Available Purpose. To report a case of Alternaria alternata keratitis in a patient with a corneal transplant in her right eye due to bullous pseudophakic keratopathy. Methods. A 66-year-old female underwent a full-thickness keratoplasty in her right eye due to bullous pseudophakic keratopathy. Three weeks after keratoplasty, epithelial edema and a stromal opacity with an infiltrate and development of peripheral corneal opacities appeared. The diagnosis of Alternaria alternata keratitis was made. Results. The patient underwent a second keratoplasty, due to the corneal melting as a result of the fungal infection. She was also given combined antifungal treatment locally and systematically. Conclusion. Corneal transplantation alone would not have been sufficient to keep the fungus in the anterior portion of the eye. Combined antifungal treatment, locally and systematically, was important in attempting to prevent the further spread of the fungus to the interior of the eye. To our knowledge, the case presented here is only the second one in the literature concerning a keratomycosis due to Alternaria alternata corneal transplant infection.

  3. Dez anos de doação de córneas no Banco de Olhos do Hospital São Paulo: perfil dos doadores de 1996 a 2005 Ten years of corneal donation to the Hospital São Paulo Eye Bank: characteristics of cornea donors from 1996 to 2005

    Directory of Open Access Journals (Sweden)

    Consuelo Bueno Diniz Adán

    2008-04-01

    corneal tissue for transplant is still an important issue in Brazil. The Hospital São Paulo Eye Bank aims to provide high-quality ocular tissues for surgery starting with rigorous selection of donors. PURPOSE: To investigate the characteristics of cornea donors to the Hospital São Paulo Eye Bank during a 10-year period. METHODS: Retrospective analysis of Hospital São Paulo Eye Bank records from January 1996 to December 2005. RESULTADS: 3,624 corneas were evaluated by the Hospital São Paulo Eye Bank during this 10-year period. Mean age of donors was 56.8 years the highest proportion being in the 70-79 years age category. The most common cause of death was cardiovascular disease followed by cancer and trauma. Mean "enucleation" and "preservation" times were 3.8 and 3.6 hours, respectively. The number of corneas received by our eye bank from other eye banks decreased substantially whereas the number of corneas harvested by the Hospital São Paulo Eye Bank staff increased. Optisol GS® were the most utilized preservation media and a high proportion of corneas classified as "Excellent" or "Good" came from young donors. CONCLUSIONS: In a period of 10 years, there was a decreasing trend in the number of donated corneas provided by other eye banks to the Hospital São Paulo Eye Bank and an increase in the number of corneas harvested and processed by the Hospital São Paulo Eye Bank staff with a significant reduction of preservation time. Most donors were male and the main cause of death was cardiovascular disease. Although a great proportion of donated corneas came from older donors, the majority of corneas used for optical transplants came from younger ones.

  4. Ultrastructure observation of rhesus bone marrow mesenchymal stem cell after transplantation of cornea%恒河猴骨髓间充质干细胞角膜移植后的超微结构观察

    Institute of Scientific and Technical Information of China (English)

    韦春玲; 孙晓梅; 杨忠昆; 代解杰; 刘海; 吉祥; 胡竹林

    2011-01-01

    连接较前紧密,均为单细胞层;角膜植片内表面可见BrdU抗体染色阳性的细胞;对照组扫描电子显微镜检测角膜植片内皮表面无细胞生长,弹性纤维裸露,BrdU染色阴性。 结论 BMSCs通过离心沉淀法移植于角膜内表面可以存活并增生为单细胞层。%Background The quest to look for seed cells is a hot spot of cornea transplant research in solving the problem of the lack of donor. Bone marrow mesenchymal stem cells(BMSCs) have been successfully induced into retinal ganglion cells(RGCs) in vivo,but the successful induction of BMSCs into corneal endothelial cells has not been reported.Objective This experiment was to study the transplantation of BMSCs on corneal endothelial surface using the splitting Descemet's membrane. Methods Four healthy adult rhesus monkeys were divided into the experimental group ( 3 monkeys) and control group ( 1 monkey). Mesenchymal stem cells (MSCs) were isolated from bone marrow by density gradient centrifugation combined with adhering means. The cultured cells were identified by flow cytometry and its ability to differentiate was determined by allowing them to differentiate into adipocytes in vitro and labeled by 5-bromodeoxyuridine ( BrdU ) for subsequent identification. Corneal grafts of 7 mm in size with tearing of the Descemet' s membrane were prepared in the experimental group and control group. After labeling by 5-bromodeoxyuridine( BrdU ) ,cultured cells were transplanted onto the endothelial surface of cornea grafts in the experimental group, but no cultured cells were seeded in the graft of the control group. The corneal grafts were then sutured in situ, and were removed 1,2 or 3 months after operation to examine the distribution and connection between transplanted cells and their morphologic changes under the electron microscope. Results High purity MSCs were harvested by density gradient centrifugation combined with adhering method. Cultured cells reached confluency

  5. 先天性无虹膜的角膜病变%Aniridia-related keratopathy

    Institute of Scientific and Technical Information of China (English)

    赵晓彬; 晏晓明

    2006-01-01

    先天性无虹膜是一种少见的先天异常,病变不仅涉及虹膜,还包括角膜、晶状体、视网膜及视神经.先天性无虹膜的角膜病变(ARK)可导致角膜基质瘢痕形成,视功能丧失.本文就ARK的特征、发病机制及治疗做一综述.

  6. Keratopathy and pachymetric changes after photorefractive keratectomy and vitrectomy with silicone oil injection

    DEFF Research Database (Denmark)

    Buch, H; Vesti Nielsen, N

    2000-01-01

    We present a man who, after bilateral excimer laser photorefractive keratectomy (PRK) for high myopia in the right eye, had repeated retinal detachment surgery with lensectomy and injection of silicone oil. Visual acuity fluctuated in accordance with significant central corneal thickness diurnal...

  7. The ethical debate and countermeasures during cornea transplantation in China%我国开展角膜移植手术过程中存在的伦理争论探讨及对策

    Institute of Scientific and Technical Information of China (English)

    隋东明; 张晶晶

    2012-01-01

    OBJECTIVE To investigate the ethical debate and countenneasures during cornea transplantation in China. METHODS Attempted to explore bioethical issues from the relationship among donor, recipient and doctor, and analyzed its root causes, finally, put forward some suggestions how to avoid ethical risks in technology. RESULTS At present ethics argued mainly included: the donor faced the voluntary donation principle received the criticism and questioned, the donor families hoped to obtain some information corresponding receptors. Receptor faced that the heterogeneity transplantation caused the identity of acceptor's personality more complication. Doctor faced how grasping the death standard; donor and the relative whether knew the circumstances and both agree; how to unify both sides benefit of the donor and the acceptor. CONCLUSION According to the aspects of ethics controversy, we suggest: establishing some reasonable ethical principles and guidelines; establishing organ donor incentives; specification and construction of eye bank; establishing and strengthening mechanisms for monitoring the work of organ transplantation.%目的 探讨我国开展角膜移植手术过程中存在伦理争论及其解决措施.方法 从供体、受体、医生三者关系出发,探讨生命伦理问题,剖析其产生根源,并对如何规避生命科技发展中的伦理风险提出建议.结果 目前伦理争论主要包括:供体面临的伦理问题是自愿捐赠原则受到批评与置疑;供体家属希望获得相应受体的资料.受体面临的伦理问题是异种移植使受体人格同一性问题更加复杂化.医生面临的伦理问题是如何把握合理死亡标准;捐献者及其亲属是否知情同意;供体与受体双方利益如何统一.结论 根据目前存在的伦理争论,提出相应解决措施:建立合理的伦理规范原则;建立器官供体激励机制;规范和建设眼库;建立和加强对器官移植工作的监督机制.

  8. Are our cornea waiting lists dynamic enough?

    Science.gov (United States)

    Palamar, Melis; Egrilmez, Sait; Yagci, Ayse

    2016-04-01

    The purpose of this study was to evaluate the dynamic reality of the corneal transplant (CT) waiting list and if the CT candidates are really eager to operation. We evaluated the response of consecutive 96 CT candidates to call for operation. The age, functional visual acuity, CT indication, waiting period, and the excuse of refusal for candidates that postponed the operation were also evaluated. The patients were classified into two groups according to the availability (Group 1, available; Group 2, unavailable). To find an available candidate for transplantation of 48 consecutive corneal tissues, 96 patients had to be called. The mean age of the patients was 57.49 ± 18.75. The mean age of Group 1 and Group 2 was 54.28 ± 18.12 (13-80) and 60.44 ± 19.01 (9-88) years, respectively (p = 0.108). It was found that the call refusal was higher in patients elder than age 70 (p = 0.04). The mean time between being added to the list and the call was 22.96 ± 7.80 (9-39) months for Group 1, 27.02 ± 8.25 (9-53) months for Group 2 (p = 0.015). The estimation point for waiting period was 22 months (p = 0.044). The most common reasons of excuse were inconvenience of timing, giving up operation, and secondary health problems, whereas anticoagulant use was the most common reason for postponement. As the waiting period gets longer and CT candidates get older, they tend to give up operation. The secondary health problems and use of systemic anticoagulants are important problems that might cause postponing the CT surgery and ruin the dynamicity of the waiting lists. PMID:26208680

  9. Facts about the Cornea and Corneal Disease

    Science.gov (United States)

    ... a donor. This procedure is used for: Fuchs’ dystrophy Post-cataract edema Corneal failure after surgery for cataract, glaucoma or retinal detachment Corneal transplants are generally done under local ...

  10. Idiopathic Sweet’s Syndrome Accompanying Bullous Lesions: Case report

    OpenAIRE

    Akı, Tuba; Karıncaoğlu, Yelda; Özcan, Hamdi; Seyhan, Muammer Eşrefoğlu; Kalaycı, Bülent; Karadağ, Neşe

    2004-01-01

    Sweet's syndrome is a rare dermatosis which is characterized by painful red plaques, pyrexia, elevated neutrophil count, and acellular infiltrate consisting predominantly of neutrophils that are diffusely distributed in the upper dermis. It may be either associated with upper respiratory tract infections, hematologic malignancies, drug hypersensitivities, sarcoidosis, autoimmune disorders or may be idiopathic. In this case an idiopathic SS that is associated with bullo...

  11. [Results of surgery in bullous emphysema (author's transl)].

    Science.gov (United States)

    Paris, F; Padilla, J; Blasco, E; Canto, A; Zarza, A G; Pastor, J; Tarazona, V

    1980-01-01

    The authors report a series of 20 patients with single (8) or multiple (12) bullae. In most cases, exeresis of these bullae favorably affected functional signs. Standard spirometry (FEV1, VC) was improved significantly in long-term studies. PMID:7465983

  12. PSEUDOSINDACTILIA EN EPIDERMÓLISIS BULLOSA Pseudosyndactylia in bullous epidermolysis

    OpenAIRE

    Enríque Vergara Amador; Henry Solaque

    2009-01-01

    La epidermólisis bullosa comprende un grupo de patologías que se caracterizan por la fragilidad de la piel, formación de ampollas, y en las formas distróficas, la formación de pseudosindactilias. Debido a lo exótico de la entidad y la dificultad para el enfoque de tratamiento, se presenta un caso clínico con desarrollo de pseudosindactilias, característico usualmente de las formas distróficas. Se describe en aspectos clínicos de la entidad, el tratamiento quirúrgico de las pseudosindactilias ...

  13. BULLOUS MYRINGITIS: AN ENIGMATIC DISEASE AND INSIGHTS INTO ITS MANAGEMENT

    Directory of Open Access Journals (Sweden)

    R Santosh Kumar

    2014-10-01

    Full Text Available Myringitis bullosa is an unusual condition of the tympanic membrane which can be easily confused with Acute Otitis media and another common entity found in hot, humid climates of our Indian subcontinent namely Granular Myringitis. Granular Myringitis is the product of continuous scratching of the drum (usually done with a feather! with the resultant granulation and may end up in scarring the drum. On the other hand pathogenesis of Myringitis bullosa is a very poorly understood and presentation is rare enough not even to have been subjected to scrutiny of any major Randomized clinical trial.

  14. EYE CLINICAL STUDY IN SWINE CORNEA IMPLANT DOGS TREATED WITH 2-1 BETAMETHASONE DISSODIC FOSPHATE AND DISSODIC FOSPHATED/ACETATE DEXAMETHASONE ESTUDO CLÍNICO NOS OLHOS DOS CÃES, TRATADOS COM 2-1 FOSFATO DISSÓDICO DE BETAMETASONA E FOSFATO DISSÓDICO/ACETATO DE DEXAMETASONA, QUE RECEBERAM IMPLANTES COM CÓRNEAS DE SUÍNOS

    Directory of Open Access Journals (Sweden)

    Nilo Sérgio Troncoso Chaves

    2007-09-01

    Full Text Available

    Ten mixed breed dogs, with a 10 kg average weight, were selected at Centro de Zoonoses de Goiânia. After being examined and considered healthy the dogs were enumerated from 11 to 20 on their collars. Each animal had swine cornea implanted into the left eye. The swine cornea was obtained at Casa de Banha Caçula, a slaughterhouse, and conserved in humid chamber for a maximum 6 hour period. Before surgery, each animal received 4 mg dissodic fosphate/acetate dexamethasone IM and was instilled one drop of betamethasone dissodic fosphate and one drop of gentamicin sulphate, maintaining the ocular medication 4 times/day and the intramuscular medication once a week, until euthanasia. After xenografts being realized, each cornea was examined every 7 days using direct ophthalmoscope to analyse transparency, vessels new formation, episcleral and conjunctival vessels reaction, cornea thickness, depth and presence or absence of deposits into the anterior chamber. On day 7 clinical evaluation, all cornea xenotransplant eyes (100% had lost transparency (++,+++, there was episcleral vessels reaction (P, anterior chamber depth was preserved (N, there wasn’t edema, as well as deposits (A on anterior chamber. On day 14, with the loss of two corneas by “self-trauma”, the other dogs had their implanted corneas (80% completely opaque (+++, with important ciliary reaction (P, edema (A; however, the anterior chamber integrity was preserved (N, without presence of deposits (A. With three more dogs euthanized between days 14 and 21, due to corticosteroids side-effects, the remaining dogs (50% preserved their implants with the day 14 alterations until the end of this experience.

    Foram utilizados 10 cães, sem ra

  15. CHARACTERIZATION OF ANTERIOR SEGMENT OPHTHALMOLOGIC LESIONS IDENTIFIED IN FREE-RANGING DOLPHINS AND THOSE UNDER HUMAN CARE.

    Science.gov (United States)

    Colitz, Carmen M H; Walsh, Michael T; McCulloch, Stephen D

    2016-03-01

    Cetaceans in the wild and under human care develop a variety of ocular lesions. Although they have echolocation, cetacean species have good sight, making ocular health an important part of overall health care. The cornea is the primary site of abnormalities in both populations. Typical lesions of cetaceans under human care are characterized in this retrospective review of cases. One hundred eighty animals (n = 360 eyes) were chosen from the author's ophthalmologic examination reports from different geographic areas; they included Atlantic bottlenose dolphins (Tursiops truncatus), Pacific bottle nose dolphins (Tursiopstruncatus gilli), Indopacific bottlenose dolphins (Steno bredanensis), Indopacific humpback dolphins (Sousa chinensis), and roughtooth dolphins (Steno bredanensis). These animals were examined at least once, although most were examined numerous times over many years; lesions were categorized and are described. Seventy-seven eyes from 47 animals were normal. Medial keratopathy was the most common lesion and identified in 180 eyes from 97 animals, with 83 affected bilaterally. Horizontal keratopathy was identified in 69 eyes from 41 animals, with 28 affected bilaterally. Axial keratopathy and nonspecific axial opacities were identified in 67 eyes from 44 animals, with 21 affected bilaterally. Seventy-eight eyes from 50 animals, with 28 affected bilaterally, had more than one type of corneal lesion. Cataracts were identified in 32 eyes from 19 animals, with 13 affected bilaterally. Traumatic injuries were also common and involved eyelids and cornea. Sixteen eyes from 11 animals were blind; five dolphins were blind bilaterally due to phthisis bulbi secondary to corneal perforation or severe trauma. None of the diseases had a sex predisposition; however, medial keratopathy was significantly more common as a bilateral presentation than as a unilateral presentation. Cetaceans under human care with impaired sight can use echolocation; however, ocular health

  16. Avaliações clínica e morfométrica da capacidade angiogênica da membrana de quitosana em córneas de coelhos Clinical and morphometric evaluations of the angiogenic capacity of chitosan membrane in rabbit corneas

    Directory of Open Access Journals (Sweden)

    Thiago André Carreo Costa

    2006-12-01

    Full Text Available OBJETIVO: Estudar a vascularização corneal (VC induzida pela membrana de quitosana (MQ implantada por enxertia interlamelar na córnea de coelhos. MÉTODOS: Foram utilizados 16 coelhos. No olho esquerdo procedeu-se enxertia interlamelar de um fragmento de 0,25 x 0,25 cm de MQ (olho tratado. No olho direito realizou-se apenas a microbolsa estromal (olho controle. Avaliaram-se clinicamente os animais aos 1, 3, 7, 15 e 30 dias de pós-operatório. Aos 30 dias mensurou-se a VC pelo Sistema Analisador de Imagens LEICA QWIN-550®. RESULTADOS: Aos sete dias observou-se VC a 1,5±0,93 mm do limbo em direção ao eixo visual. Aos 15 dias houve aumento da VC (4,75±3,20 mm, que se manteve aos 30 dias (4,25±4,10 mm. Os olhos controles não apresentaram quaisquer alterações oculares. Houve diferença estatística (pPURPOSE: To evaluate corneal vascularization (CV induced by interlamellar graft chitosan membrane (CM in rabbit cornea. METHODS: An interlamellar graft with a 0.25 x 0.25 cm CM fragment was performed in the left eye (treated eye. In the right eye, an estromal tunnel was done (control eye. The clinical evaluation was done at 1, 3, 7, 15 and 30 days postoperatively. CV analysis was after 30 days by the Images Analizator System LEICA QWIN-550®. RESULTS: After 7 days, CV at 1.5±0.92 mm from the limbus in direction of the cornea axial area was observed. After 15 days CV increased (4.75±3.19 mm, remaining until day 30 (4.25±4.06 mm. The control eyes did not present any changes. There was a statistical differences of the vascularizated corneal areas between control and treated eyes from the 15th to the 30th postoperative day. CONCLUSION: The chitosan membrane induced corneal angiogenesis when applied to rabbit cornea through an interlamellar graft, which was maintained at low levels until 30 days postoperatively. Although further studies are necessary, the results found here demonstrated the usefulness of chitosan membrane in keratoplasties.

  17. The morphology and thickness of cornea in patients with Marfan syndrome%马方综合征患者角膜形态及厚度的特点

    Institute of Scientific and Technical Information of China (English)

    刘秋平; 张广斌; 邵毅; 易敬林; 刘祖国; 谭叶辉; 陈伟; 毛祖红; 王乐

    2011-01-01

    Objective To search for the characteristics of MFS in corneal morphology and thickness. Methods Twenty-four patients (48 eyes) with MFS and 24 healthy age- and gender-matched volunteers (48 eyes) were recruited in this clinical prospective, and comparative series study. Firstly,biomicroscopic examination and Type-A ultrasonometry was conducted to search for ectopia lentis and axis length. Secondly, the corneal morphologic parameter[including the height of anterior and posterior surface,the centre corneal curvature, the mean astigmatism in the 3.0-mm central zone (Mean A), the mean simulated astigmatism (Sim A) , the mean keratometry in the 3.0-mm central zone (Mean K), the mean simulated keratometry (Sim K), the 3.0-mm zone irregularity (3.0ZI), the 5.0-mm zone irregularity (5.0ZI) ,corneal thickness index (CTI)] and thickness (at the central location and at eight midperipheral locations) were obtained by the the autorefractometer and the Orbscan Ⅱ Z corneal topography. Last, the statistics method including Crosstabs, One-way ANOVA, student-t test and discriminant analysis were applied and the correlations were established. Results There is no statistically significance between MFS group and control group in ages (38 ±7) and (37 ±8) years, gender (8/16) and (9/15), and axis length (23. 12 ±1.06) mm and (24. 26 ±2. 96) mm (age x2=0.091 ,P=0.763 ;gender t=0.324, axis length t=1.976,P >0.05). Flat cornea ratio (66. 7% and 12. 5%) and topography of the oval (25.0% and 16. 7%),irregular bow-shaped (41.7% and 37.5%) and irregular-shaped (12. 5% and 8. 3%) were increased significantly in patients with MFS. The corneal topography(MFS/control) showed that there are statistically significance in the thinnest thickness of cornea (489. 8 ± 42. 9) μm and (544. 8 ± 25.7) μm, Mean K (40.60±1.30) Dand (42.80± 1.40) D, Sim K (40.50±1.30) D and (42.80±1.20) D, Sim A(1.08 ± 0.86)D and (0.91 ±0.46) D, CTI 1.57±0.24 and 1.21 ±0.14, 3.0ZI (1.76±0.96) D and (1.54 ±0

  18. Comparison of pharmacokinetics of levofloxacin in tears and cornea of rabbits after oral or intravenous administration%左氧氟沙星口服或静脉注射家兔泪液、角膜组织的药动学比较

    Institute of Scientific and Technical Information of China (English)

    万霞; 周远大; 何海霞; 李娟

    2012-01-01

    AIM To compare pharmacokinetics of levofloxacin in tears and cornea of rabbit after oral or intravenous administration. METHODS Fifty-four rabbits were randomly divided into two groups: oral group and intravenous group (n = 27) given levofloxacin 24 mg·kg-1 with different administrations. Their tears and corneal samples were collected at different time point after administration. Levofloxacin concentrations in tears and cornea were detected by HPLC, and pharmacokinetics parmeters were calculated by the 3p97 sowftware program. RESULTS After oral administration, the main pharmacokinetic parmeters of levofloxacin in tears and cornea were as followed: tmax (0.58 ± 0.20) and (1.00 ±0.55) h, cmax (34.27 ± 11.69) and (8.51 ± 2.72) μg· g-1, AUC0_t (157.19 ± 32.15) and (22.90 ±2.63) μg·h·g-1, respectively. After intravenous injection, tmax (0.83 ±0.26) and (0.83 ± 0.26) h, cmax (30.44 ±3.18) and (6.12 ±0.71) μg·g-1, AUCV0-t, (136.72 ± 20.12) and ( 26.01 ± 1.59) μg · h · g-1, respectively. There were no significant differences in concentrations and pharmacokinetics parmeters of levofloxacin in tears and corneal tissue after oral or intravenous administration (P > 0.05). CONCLUSION After oral or intravenous administration of levofloxacin, similar pharmacokinetics parmeters were observed in the tears and corneal tissue of rabbits.%目的 家兔口服或静脉注射(静注)左氧氟沙星后泪液与角膜组织的药动学比较.方法 选取54只家兔分成口服组和静注组(n=27),分别口服或静注左氧氟沙星24 mg ·kg-1,不同时间采集泪液或角膜组织,高效液相色谱法测定药物浓度,3p97软件计算药动学参数.结果 口服给药后泪液和角膜组织中左氧氟沙星tmax、cmax和AUC0-t分别为(0.58±0.20)与(1.00±0.55)h、(34.27±11.69)与(8.51±2.72) μg·g-1、 (157.19±32.15)与(22.90±2.63) μg·h·g-1;静注后分别为(0.83±0.26)与(0.83±0.26)h、(30.44±3.18)与(6.12±0.71) μg·g-1、(136.72±20.12)

  19. ESTUDO DO LEUCOGRAMA COMO MONITOR DO 2-1 FOSFATO DISSÓDICO DE BETAMETASONA DO FOSFATO DISSÓDICO/ACETATO DE DEXAMETASONA, EM CÃES QUE RECEBERAM TRANSPLANTES COM CÓRNEAS DE SUÍNOS WHITE BLOOD COUNT AS A 2-1 BETAMETHASONE DISSODIC FOSFHATE AND DEXAMETHASONE DISSODIC FOSPHATE/ACETATE MONITOR IN SWINE CORNEA TRANSPLANTED DOGS

    Directory of Open Access Journals (Sweden)

    Larissa Franco de Araújo

    2007-09-01

    . Each animal had swine cornea implanted into the left eye. The swine cornea was obtained at Casa de Banha Caçula, a slaughterhouse, and conserved in a humid chamber during a maximum 6 hour period. Each animal received 4 mg dissodic fosphate/acetate dexamethasone IM and was instilled one drop of gentamycin sulphate/betametbasone dissodic fosphate, maintaining ocular medication 4 times/day and intramuscular medication once a week until euthanasia. Each animal had blood samples collected on day 0, before surgery, and on day 14 and 28 to analyse white blood count as a monitor of corticosteroids, imunocellular supressor drugs. From ten blood samples collected on day 0, 80% didn’t present any alterations, 10% presented leukocytosis with neutrophilia and 10% weren’t submitted to analysis. Due to the fact that three animals had been euthanized between days 14 and 21, these samples (40%, were analyzed and compared with these collected on day 0. Five animals (50% had their samples examined on day 28. On both days 14 and 28, there were 100% neutrophilia, 100% eosinophilia and 44.4% lymphocytopenia, indicating imunocellular supression.

    KEY-WORDS: Corticosteroids; xenotransplants; cornea; canine; swine.

  20. Effects of mitomycin C on infiltration of polymorphonuclear leukocytes after epithelial scrape injury in the mouse cornea Efeito da mitomicina C na infiltração de leucócitos polimorfonucleares após lesão epitelial em córnea de camundongo

    Directory of Open Access Journals (Sweden)

    Ana Cecília Souza Leão Escarião

    2008-12-01

    Full Text Available PURPOSE: To determine whether mitomycin C (MMC alters appearance and disappearance of polymorphonuclear leucocytes (PMN in the cornea stroma, using an epithelial scrape injury in eye mouse model. METHODS: Twenty-mice underwent mechanical epithelium debridement in the central cornea using 20% ethanol. After the scrape, the right eye received 0.02% MMC for one minute, while the left eye received physiological saline. The animals were sacrificed on days 1, 2, 5, and 14 after surgery, and corneal whole mounts were prepared for histology. PMN distribution was analyzed in digitized microscope images. Cell division in the cornea was determined by immunohistochemical detection of bromodeoxyuridine (BrdU, which was injected intraperitoneally before the mice were sacrificed. RESULTS: Epithelial scrape injury triggered infiltration of PMNs into the corneal stroma. An analysis of PMN distribution revealed that there was no difference between eyes treated with and without MMC at all time points. BrdU labeling showed that 0.02% MMC for one minute blocked keratocyte proliferation completely. CONCLUSION: MMC treatment regimen, which is common in clinical practice, inhibits keratocyte proliferation during wound healing, but when used at 0.02% for one minute, it does not affect PMN infiltration into the corneal stroma, and subsequent movement toward the injury site, or the disappearance of PMNs from the stroma, in the mouse epithelial injury model.OBJETIVO: O objetivo do estudo foi determinar se a mitomicina C (MMC altera o aparecimento dos leucócitos polimorfonucleares (PMN no estroma corneano após abrasão epitelial central, utilizando olhos de camundongo como modelo. MÉTODOS: Vinte camundongos foram submetidos à abrasão epitelial em córnea central utilizando etanol a 20%. Após a lesão, o olho direito recebeu MMC a 0,02% por 1 minuto, enquanto o olho esquerdo recebeu solução salina. Os animais foram sacrificados em 1, 2, 5 e 14 dias após a cirurgia e

  1. OCULAR MICROBIAL POPULATION AS A 2-1 BETAMETHASONE DISSODIC FOSPHATE AND DISSODIC FOSPHATE/ACETATE DEXAMETHASONE MONITOR IN SWINE CORNEA TRANSPLANTED DOGS ESTUDO DA MICROBIOTA OCULAR COMO MONITOR DO 2-1 FOSFATO DISSÓDICO DE BETAMETASONA E DO FOSFATO DISSÓDICO/ACETATO DE DEXAMETASONA, EM CÃES QUE RECEBERAM TRANSPLANTES COM CÓRNEAS DE SUÍNOS

    Directory of Open Access Journals (Sweden)

    Nilo Sérgio Troncoso Chaves

    2007-09-01

    Full Text Available

    Ten mixed breed dogs, with a 10 kg average weight, were selected at Centro de Zoonoses de Goiânia. After being examined and considered healthy, the dogs were enumerated from 11 to 20 on their collars. Each animal had swine cornea implanted into the left eye. The swine cornea was obtained at Casa de Banha Caçula, a slaughterhouse, and conserved in a humid chamber during a maximum 6 hour period. Before surgery, each animal received 4 mg dissodic fosphate/acetate of dexamethasone IM and one drop of betamethasone dissodic fosphate and gentamycin sulphate. This medication was kept until euthanasia. Each dog had a superior fornix sample collected, the sample was immersed in 0.1% peptonized water, so that tests for aerobic bacteria and fungus could be realized. Each test was repeated with samples collected on days 7, 14, 21 and 28, after graft, and compared with those from day 0. The 12.8% Staphylococcus epidermidis on initial samples was caused by contamination during collection because it wasn’t found on the 28th day samples. This fact suggests that, although corticosteroids had caused systemic imunocellular supression, they didn’t make easier the bacterial development into the grafted eye.

    KEY-WORDS: Microbial population; corticosteroids; xenotranplants; cornea; canine; swine.

    Foram utilizados dez cães, sem raça definida, com peso médio de 10kg, selecionados no Centro de Zoonoses da Prefeitura de Goiânia. Após examinados e considerados sadios, foram numerados de 11 a 20 na respectiva coleira. Cada cão, a seu tempo, teve implantada no olho esquerdo córnea de suíno, este abatido no frigorífico Casa de Banha Caçula. A córnea foi conservada em câmara úmida pelo

  2. Influence of topical carbonic anhydrase inhibitor on the expression of aquaporin-1 in rat cornea with neovascularization%碳酸酐酶抑制剂的局部应用对大鼠角膜新生血管形成过程中水通道蛋白1表达的影响

    Institute of Scientific and Technical Information of China (English)

    张洁; 李立

    2011-01-01

    (t=2.48,P=0.02),2个组AQP1灰度值分别为88.01±11.03和58.10±12.14,差异有统计学意义(t=9.99,P=0.00).结论 布林佐胺滴眼液能抑制大鼠角膜碱烧伤后CNV形成过程中AQP1的高表达,从而间接影响VEGF的表达,抑制或延缓CNV的形成.%Background Researches showed that aquaporin-1 (AQP1) is closely associated with corneal neovescularization(CNV).Carbonic anhydrase inhibitor has the inhibitory effect on the AQP1 and further suppresses the CNV.However,the systemic adverse effect of Carbonic anhydrase inhibitor limit its clinical application.Therefore,the influence of topical carbonic anhydrase inhibitor on CNV is concerned.Objective Present study was to investigate the effects of topical carbonic anhydrase inhibitors on the expression of AQP1 in rat cornea after alkali burn and explore its role in corneal neovascularization (CNV).Methods The alkali-burn animal models were established in 60 eyes of 30 clean Sprague Dawley rats by putting the filter paper soaked 1 mol/L NaOH solution at the central cornea for 40 seconds.1% Brinzolamide was topically administered in the 30 eyes of 15 models (Brinzolamide group),and the normal saline solution was used at the same way in other 30 eyes of 15 rats (model group).The 10 eyes of 5 normal Sprague Dawley received the eye drops of normal saline solution as the normal control group.The corneal burning degree was graded on the Mahoney ' s criteria in the third day,and Ee ' s method was used to score the opacification of cornea and the CNV area was analyzed in 3,5,7,10 days under the slit lamp microscope.The cornea tissue was obtained in the tenth day after burning for the observation of the pathology under the light microscope and the ultrastructure under the transmission electron microscope.The expressions of AQP1 and vascular endothelial growth factor(VEGF) in cornea tissue were detected using immunohistochemistry.The use of animals complied with the Statement of ARVO.Results No significant

  3. Análise da qualidade das córneas doadas e do intervalo entre óbito, enucleação e preservação após a implantação de novas normas técnicas e sanitárias em Banco de Olhos Universitário Comparative analysis of the donor cornea quality and of the interval between death and preservation before and after new sanitary and technique rules in a University Eye Bank

    Directory of Open Access Journals (Sweden)

    Fabio Zantut

    2012-12-01

    Full Text Available OBJETIVO: Comparar o intervalo entre o óbito e a enucleação (ΔT-O-E, entre a enucleação e a preservação (ΔT-E-P e a qualidade da córnea antes e após a implantação de novas normas técnicas e sanitárias baseadas na Resolução RDC 347. MÉTODOS: Estudo retrospectivo em que foram avaliados os prontuários dos doadores de córnea do Banco de Tecidos Oculares da Santa Casa de São Paulo, 2 anos antes e 2 anos depois da implementação de novas normas sanitárias. RESULTADOS: Foi observado aumento do número absoluto de 205 para 374 doadores após as mudanças adotadas. Não foi observada diferença estatisticamente significante no Δt-O-E e ΔT-E-P antes e após as mudanças implantadas. Do total de 1.105 córneas doadas, foi observado 388 córneas doadas antes das mudanças e 717 córneas doadas após as mudanças implementadas. Foi observado aumento estatisticamente significante da graduação da qualidade da córnea doada de 1,76 ± 0,90 para 1,94 ± 0,88 após a implementação das novas normas da Resolução. CONCLUSÃO: Após as mudanças técnicas e sanitárias exigidas pela Resolução 347, houve grande aumento no número de córneas doadas, captadas e preservadas. O Banco de Tecidos Oculares não diminuiu os ΔT O-E e ΔT E-P. A qualidade da córnea apresentou-se inferior após as mudanças realizadas no setor.PURPOSE: To compare the interval between death and enucleation (ΔT-O-E, between enucleation and preservation (ΔT-E-P and the quality of the cornea before and after the implantation of new technique and sanitary rules. METHODS: A retrospective study that evaluated the records of cornea donors in Sao Paulo's Santa Casa Eye Tissue Bank 2 years before and 2 years after the implementation new sanitary rules. RESULTS: An increase was observed in the absolute number of 205 to 374 donors following the adopted changes. There was no statistically significant difference in Δt-O-E and ΔT-E-P before and after the implemented

  4. Endothelial cell study in a case of Werner′s syndrome undergoing phacoemulsification and Yettrium-Aluminum-Garnet laser capsulotomy

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    Vasudha Kemmanu

    2012-01-01

    Full Text Available Werner′s syndrome (WS is a rare autosomal recessive disorder with multisystem manifestations of premature aging from the second decade of life. Cataract is one of the features of WS. Cataract surgery is complicated with postoperative wound dehiscence and bullous keratopathy when the surgery is done by intracapsular or conventional extracapsular method. We report the short-term result of phacoemulsification and Neodymimum Yettrium-Aluminum-Garnet laser (Nd YAG capsulotomy in a case of WS with bilateral cataracts. Postoperatively and post capsulotomy, there was no change in the endothelial cell morphology. There was an 8.6% decrease in endothelial cell count at the end of 15 months postoperatively and 11 months post YAG capsulotomy. This decrease is within the acceptable range of cell loss after phacoemulsification and YAG capsulotomy. To the best of our knowledge, this is the first reported case of YAG laser capsulotomy in WS.

  5. Lentes de contato terapêuticas e as vantagens dos materiais de alto Dk Therapeutic contact lenses and the advantages of high Dk materials

    Directory of Open Access Journals (Sweden)

    Cleusa Coral-Ghanem

    2008-12-01

    Full Text Available As lentes de contato terapêuticas são úteis para o tratamento de uma série de doenças da superfície ocular. Suas principais finalidades são: reduzir a dor; proteger a córnea; facilitar e manter a cicatrização epitelial; restaurar a câmara anterior e liberar medicamentos na superfície ocular. Há uma variedade de tipos e materiais, sendo que a escolha depende da doença a ser tratada, tempo de uso e necessidades fisiológicas do olho doente. Doenças como a ceratopatia bolhosa, erosão recorrente do epitélio corneano, olho seco e defeitos epiteliais pós-operatórios, além de várias outras doenças que afetam a superfície ocular podem ser tratadas com a utilização das lentes de contato terapêuticas. Elas não devem ser utilizadas em processos infecciosos ou em pacientes que estão impossibilitados de retornar para acompanhamento regular. A adaptação, controle e manutenção adequados das lentes de contato terapêuticas são fundamentais para prevenir complicações, como: neovascularização corneana, conjuntivite papilar gigante ou ceratite infecciosa. O controle e manutenção foram facilitados com o advento das lentes de contato terapêuticas de silicone-hidrogel. Essas lentes permitem trocas menos freqüentes devido a sua maior oxigenação e, em casos selecionados, aceleram a reepitelização corneana.Therapeutic contact lenses are useful in a variety of ocular surface diseases. Their main indications are: to relieve the pain; protect ocular surface; promote corneal healing and epithelial regeneration; seal a leaking corneal wound and deliver ophthalmic drugs on the ocular surface. There are several kinds of lens designs and materials, and their choice is dependent on the specific disease to be treated, the duration of treatment and the physiologic needs of the diseased cornea. Bullous keratopathy, recurrent epithelial erosion syndrome, dry eye and postoperative epithelial defects are amongst their indications

  6. Corneal changes in ectrodactyly-ectodermal dysplasia-cleft lip and palate syndrome: case series and literature review.

    Science.gov (United States)

    Felipe, Anthony F; Abazari, Azin; Hammersmith, Kristin M; Rapuano, Christopher J; Nagra, Parveen K; Peiro, Baltasar Moratal

    2012-10-01

    The aim of this study is to describe the corneal changes in three unrelated patients with ectrodactyly-ectodermal dysplasia-cleft lip and palate (EEC) syndrome and review the literature on the possible etiology and clinical presentation of similar cases. Case 1 is an 18-year-old female with cleft lip and palate, syndactyly, and bilateral corneal pannus superiorly and inferiorly. She was initially diagnosed and treated as herpes simplex virus keratitis. Case 2 is a 3-year-old female born with cleft lip and palate, absent radial digits in both hands, and bilateral lacrimal stenosis. She developed progressive stromal scarring and neovascularization in both eyes. Her cornea perforated after developing infectious ulceration. Case 3 is a 49-year-old male with cleft palate, claw-hand deformities, absent meibomian glands and lacrimal duct, right ankyloblepharon, and a superior wedge-shaped opacity in the left cornea. The clinical findings demonstrated the different spectrum of keratopathy seen in patients with EEC. All patients were treated medically and without any surgical intervention. Limbal stem cell deficiency (LSCD) is presumed to be the cause in all three cases. Corneal changes in EEC can have variable presentation. LSCD seems to be the etiology of such keratopathy. Recurrent infection from lacrimal drainage obstruction and tear film instability are other risk factors for disease severity and progression. PMID:22618129

  7. In vivo confocal microscopic characteristics of crystalline keratopathy in patients with monoclonal gammopathy: Report of two cases

    Directory of Open Access Journals (Sweden)

    Sibel Kocabeyoglu

    2014-01-01

    Full Text Available In this paper, we report two cases of a 62-year-old patient presented with blurred vision and a 45-year-old male diagnosed with multiple myeloma who was referred from the Department of Oncology. Slit-lamp examination, in vivo confocal microscopy (IVCM, systemic work-up and serum protein electrophoresis were obtained. In both patients, slit-lamp findings revealed bilateral diffuse subepithelial and anterior stromal crystals and IVCM showed highly reflective deposits in the corneal epithelium and stroma. The first patient was eventually diagnosed with monoclonal gammopathy of undetermined significance following bone marrow biopsy and systemic evaluation. Unusual corneal deposits may constitute the first sign of monoclonal gammopathies. IVCM may be helpful in showing the crystalline nature of the corneal deposits and guiding the clinician to the diagnosis of gammopathies. Both ophthalmologists and oncologists should be aware that corneal deposits may herald a life-threatening hematologic disease.

  8. 蜂蜜对新目安眼用凝胶中更昔洛韦在兔离体角膜中的渗透释药行为的影响%Effects of honey on the rabbits' isolated cornea osmotic releasing behaviors of Ganciclovir in Mu'an ophthalmic gel

    Institute of Scientific and Technical Information of China (English)

    赵碧清; 何群; 王适; 张湘晖; 黄略; 郭畅

    2013-01-01

    目的 研究不同浓度的蜂蜜对新目安眼用凝胶中更昔洛韦在兔离体角膜中的渗透释药行为的影响,优选新目安眼用凝胶中蜂蜜的最佳用量,为新目安眼用凝胶处方设计提供依据.方法 以谷胱甘肽缓冲液为释放介质,采用高效液相色谱法测定新目安眼用凝胶中更昔洛韦在兔离体角膜中的累积渗透释药量,绘制累积释药曲线,建立动力学模型,提取累积渗透速率t70%及累积渗透百分率Q8h.结果 含5%蜂蜜的新目安眼用凝胶与含7%蜂蜜的新目安眼用凝胶的兔离体角膜渗透速度、兔离体角膜累积渗透百分率差异均无统计学意义,但均优于含3%蜂蜜的新目安眼用凝胶.结论 新目安眼用凝胶中蜂蜜的浓度为5%为佳.%Objective To study the effects of honey on the rabbits' isolated cornea osmotic releasing behaviors of Ganciclovir in Mu'an ophthalmic gel and to optimize the dosage of honey in Mu'an ophthalmic gel,and then provide the basis for prescription design.Methods The cumulative osmotic medicine releasing amount of Ganciclovir in Mu'an ophthalmic gel in the isolated cornea of rabbits was determined by HPLC with glutathione buffer as releasing medium.The curves of medicine release were drawn and the kinetics models were established and cumulative osmotic velocity (t70%)and percentage (Q8h) were extracted.Results There were no statistical differences in t70% and Q8h of Ganciclovir between Mu'an ophthalmic gel containing 5% honey group and Mu'an ophthalmic gel containing 7% honey group,but they were higher than Mu'an ophthalmic gel containing 3% honey group.Conclusion Mu'an ophthalmic gel containing 5% honey is optimal in cumulative osmotic velocity(t70%) and percentage(Q8h).

  9. A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK-operated corneas Linha de pesquisa em fibrina e possibilidades teóricas de seu uso como membrana basal provisória em úlceras corneanas não perfuradas e em córneas operadas de ceratectomia fotorrefrativa (PRK

    Directory of Open Access Journals (Sweden)

    José Américo Bonatti

    2007-10-01

    Full Text Available PURPOSE: To report a specific line of research developed at the University of São Paulo/Brazil on fibrin glue used for sealing corneal perforations and the perspectives of use on non-perforated corneal ulcers and photorefractive keratectomy-operated corneas. METHODS: To describe fibrin glue manufacture, development of a device to test the glued perforated corneal area resistance, subsequent experimental investigations of the use of the fibrin glue in corneal perforations, reporting its efficacy, mechanical resistance experiments and histological study. Finally, the medical literature basis is searched to propose studies on the use of fibrin as a temporary basal membrane on non-perforated corneal surfaces like non-infectious corneal ulcers and on post-photorefractive keratectomy corneal surfaces. RESULTS: The development of fibrin glue, the device for resistance experiments, the efficacy, resistance and histological studies on fibrin glue used for sealing corneal perforations are reported as well as the scientific literature basis for the proposed studies on the use of fibrin as a temporary basal membrane on non-perforated corneas like non-perforated corneal ulcers and photorefractive keratectomy corneal surfaces. CONCLUSION: A specific line of research was reported on fibrin glue to seal corneal perforations at the University of São Paulo/Brazil and the theoretical perspectives for the use of fibrin in non-perforated corneal ulcers and on photorefractive keratectomy-operated corneas in an attempt to reduce corneal haze.OBJETIVO: Relatar uma linha de pesquisa desenvolvida no Brasil (Universidade de São Paulo em cola de fibrina para tamponamento de perfuração corneana e a perspectiva de seu uso em úlceras corneanas não perfuradas e em córneas operadas de ceratectomia fotorrefrativa. MÉTODOS: Relatar a fabricação da cola de fibrina, desenvolvimento de instrumentação para ensaio de resistência da área colada, estudo experimental da

  10. Effect of epithelial debridement on human cornea proteoglycans

    OpenAIRE

    E.S. Soriano; M.S.Q. Campos; J.A.K. Aguiar; Y.M. Michelacci

    2001-01-01

    Corneal transparency is attributed to the regular spacing and diameter of collagen fibrils, and proteoglycans may play a role in fibrillogenesis and matrix assembly. Corneal scar tissue is opaque and this opacity is explained by decreased ultrastructural order that may be related to proteoglycan composition. Thus, the objectives of the present study were to characterize the proteoglycans synthesized by human corneal explants and to investigate the effect of mechanical epithelial debridement. ...

  11. Diverse set of Turing nanopatterns coat corneae across insect lineages.

    Science.gov (United States)

    Blagodatski, Artem; Sergeev, Anton; Kryuchkov, Mikhail; Lopatina, Yuliya; Katanaev, Vladimir L

    2015-08-25

    Nipple-like nanostructures covering the corneal surfaces of moths, butterflies, and Drosophila have been studied by electron and atomic force microscopy, and their antireflective properties have been described. In contrast, corneal nanostructures of the majority of other insect orders have either been unexamined or examined by methods that did not allow precise morphological characterization. Here we provide a comprehensive analysis of corneal surfaces in 23 insect orders, revealing a rich diversity of insect corneal nanocoatings. These nanocoatings are categorized into four major morphological patterns and various transitions between them, many, to our knowledge, never described before. Remarkably, this unexpectedly diverse range of the corneal nanostructures replicates the complete set of Turing patterns, thus likely being a result of processes similar to those modeled by Alan Turing in his famous reaction-diffusion system. These findings reveal a beautiful diversity of insect corneal nanostructures and shed light on their molecular origin and evolutionary diversification. They may also be the first-ever biological example of Turing nanopatterns. PMID:26307762

  12. Effects of radiofrequency hyperthermia on the healthy canine cornea

    Energy Technology Data Exchange (ETDEWEB)

    Glaze, M.B.; Turk, M.A.

    1986-04-01

    Radiofrequency hyperthermia was used to induce axial corneal lesions in the eyes of 10 dogs. Clinical observations were continued for up to 6 months, using biomicroscopy and indirect ophthalmoscopy. Eyes were harvested at intervals for light and electron microscopic evaluation. Clinical alterations included immediate corneal opacification and epithelial disruption at the site of electrode contact. Ulcerative keratitis persisted for 4 to 6 days, accompanied by anterior uveitis. Additional corneal changes included stromal thinning, edema, and vascularization. Final evaluation revealed negligible alterations in corneal contour or clarity 6 months after treatment. Microscopically, epithelial and superficial stromal necrosis preceded epithelial loss. Stromal alterations included edema (associated with focal endothelial detachments), vascularization, and inflammatory cell infiltration. Recovery was characterized by keratocytic hyperplasia and hypertrophy, epithelial proliferation, and stromal condensation.

  13. Nerves and Neovessels Inhibit Each Other in the Cornea

    OpenAIRE

    Ferrari, Giulio; Hajrasouliha, Amir R.; Sadrai, Zahra; Ueno, Hiroki; Chauhan, Sunil K.; Dana, Reza

    2013-01-01

    In this study, we use two different animal models. First, we induce corneal neovascularization and notice that the development of vessels is followed by the disappearance of corneal nerves. Second, we induce loss of corneal nerves, which is followed by the development of neovessels.

  14. Photorefractive keratectomy results in myopic patients with thin cornea eyes

    Directory of Open Access Journals (Sweden)

    Hassan Hashemi

    2015-01-01

    Conclusion: PRK is a safe, effective, and predictable procedure with desirable effects on mesopic CS in patients with corneal thickness <500 μm, which increases HOAs and decreases corneal integrity proportionate to its value before the procedure.

  15. Effect of Flat Cornea on Visual Outcome after LASIK

    OpenAIRE

    Engy Mohamed Mostafa

    2015-01-01

    Purpose. To evaluate the effect of preoperative and postoperative keratometry on the refractive outcome after laser in situ keratomileusis (LASIK) for moderate and high myopia. Methods. Records of 812 eyes (420 patients) with myopia ≥−6 D who had LASIK at Sohag Laser Center, Egypt, from January 2010 to November 2013, were retrospectively analyzed. Main outcome measures were postoperative corrected distance visual acuity (CDVA), postoperative spherical equivalence, and postoperative Q factor. ...

  16. Effect of Flat Cornea on Visual Outcome after LASIK

    Science.gov (United States)

    Mohamed Mostafa, Engy

    2015-01-01

    Purpose. To evaluate the effect of preoperative and postoperative keratometry on the refractive outcome after laser in situ keratomileusis (LASIK) for moderate and high myopia. Methods. Records of 812 eyes (420 patients) with myopia ≥−6 D who had LASIK at Sohag Laser Center, Egypt, from January 2010 to November 2013, were retrospectively analyzed. Main outcome measures were postoperative corrected distance visual acuity (CDVA), postoperative spherical equivalence, and postoperative Q factor. Results. LASIK was performed in 812 eyes (mean age 21.8 ± 5.2 years). Patients were grouped according to the degree of preoperative myopia into three groups: Group 1, −6 D to −7.9 D; Group 2, −8 to −9.9 D; and Group 3, −10 to −12 D. The refractive outcome among the different myopia groups was stratified by pre- and postoperative keratometry. A trend toward greater undercorrection was noted in eyes with preoperative keratometry 46 D in all myopia groups. The undercorrection was also noted in postoperative keratometry groups <35 D. Conclusions. Preoperative and postoperative keratometry appeared to influence the refractive outcome especially in high myopic eyes. PMID:26693347

  17. [Aesthesiometry of the cornea after refractive corneal surgery].

    Science.gov (United States)

    Kohlhaas, M; Draeger, J; Böhm, A; Lombardi, M; Abbondanza, M; Zuppardo, M; Görne, M

    1992-10-01

    The corneal sensibility was examined with the aesthesiometer of Draeger in 41 patients after refractive corneal surgery, 31 patients after radial keratotomy, 5 after epikeratophakia, 5 after excimer laser ablation. It could be shown that after radial corneal incisions the sensibility remains normal. After epikeratophakia the corneal sensibility is asensible even 3 years after operation. The lenticle periphery shows an increase of sensibility after 6 months. Excimer patients with "haze" showed a significant hyposensibility in the centre. The central sensibility showed normal values after a normal corneal wound healing. PMID:1453657

  18. Integrin-dependent Neutrophil Migration in the Injured Mouse Cornea

    OpenAIRE

    Hanlon, Samuel D.; Smith, C. Wayne; Sauter, Marika N; Burns, Alan R.

    2014-01-01

    As an early responder to an inflammatory stimulus, neutrophils (PMNs) must exit the vasculature and migrate through the extravascular tissue to the site of insult, which is often remote from the point of extravasation. Following a central epithelial corneal abrasion, PMNs recruited from the peripheral limbal vasculature migrate into the avascular corneal stroma. In vitro studies suggest PMN locomotion over 2-D surfaces is dependent on integrin binding while migration within 3-D matrices can b...

  19. Asphericity of the human cornea for different corneal diameters

    OpenAIRE

    González-Méijome, José Manuel; Villa-Collar, César; Montés-Micó, Robert; Gomes, Antonio

    2007-01-01

    PURPOSE: To measure the anterior corneal asphericity (Q) with different corneal diameters. SETTING: Department of Physics (Optometry), University of Minho, Braga, Portugal. METHODS: Thirty-six eyes of 36 patients were evaluated using a videokeratoscope, and the Q-values were recorded. Topographic data were also analyzed using Vol-CT 6.89 software (Sarver & Associates, Inc) to obtain the Q-values with different corneal diameters (3.0 mm, 4.0 mm, 5.0 mm, 6.0 mm, and 7.0 mm). Variable Q models o...

  20. OCT对急性闭角型青光眼治疗前后角结膜结构的改变%Clinical observation of OCT on detecting the structural change of cornea and conjunctiva in patients with acute angle closure glaucoma

    Institute of Scientific and Technical Information of China (English)

    赵黎; 李青松; 张兴儒

    2015-01-01

    Abstract•AlM:To observe thickness and morphological changes of cornea and bulbar conjunctiva pre- and post acute angle closure glaucoma ( AACG ) therapy by optical coherence tomography ( OCT) .•METHODS: Twenty - five patients with AACG were recruited. lntraocular pressure ( lOP ) and morphological characteristic of cornea and conjunctiva were measured at their first visit, 1, 2 and 3d after they began the treatments.•RESULTS:The corneal epithelium thickness and corneal thickness of patients with acute attack of angle closure glaucoma were 72. 76±11. 95μm and 589. 40±66. 91μm; the conjunctival epithelial layer thickness was 58. 88±12. 87μm;the thickness of conjunctive stroma was 299. 76±94. 86μm;the conjunctival full thickness was 358. 64±102. 55μm. The corneal epithelium thickness and corneal thickness at the first day of the treatment were 69. 28±12. 65μm and 579. 04± 67. 88μm;the conjunctival epithelial layer thickness was 57. 04 ± 12. 05μm;the thickness of conjunctive stroma was 282. 44±91. 47μm;the conjunctival full thickness was 339. 48± 100. 28μm. the corneal epithelium thickness and corneal thickness at the second day of treatment were 66. 76 ± 11. 42μm and 563. 32 ± 63. 87μm;the conjunctival epithelial layer thickness was 54. 76 ± 11. 01μm;the thickness of conjunctive stroma was 267. 00 ± 98. 54μm;the conjunctival full thickness was 322. 16 ± 106. 12μm. the corneal epithelium thickness and corneal thickness of the third day treatment are 65. 16 ± 12. 25μm and 550. 36 ± 71. 48μm; the conjunctival epithelial layer thickness was measured 53. 36± 10. 29μm;the thickness of conjunctive stroma was 252. 76± 99. 32μm;the conjunctival full thickness was 306. 52 ± 107. 31μm. The difference of lOP, corneal epithelial thickness, corneal thickness, conjunctival epithelial layer thickness, conjunctival stroma thickness and conjunvtival full thickness had statistically significant(P<0. 05).•CONCLUSlON:The corneal and

  1. Clinical observation of alcohol lysis combined with limbus corneae epithelial transplantation in the treatment of primary pterygium%乙醇松解翼状胬肉切除联合角膜缘上皮移植的疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘绍峰; 黄志强; 方向阳; 李家琳

    2010-01-01

    目的 初步观察乙醇松解在角膜缘上皮移植治疗翼状胬肉中的临床效果.方法 随机分组对比研究.将176例(192只眼)初发翼状胬肉随机分A、B两组:A组(87例90只眼)单纯翼状胬肉切除联合角膜缘上皮移植;B组(B组89例102只眼)乙醇松解胬肉切除联合角膜缘上皮移植.对比观察两组角膜创面上皮愈合时间及复发率.结果 A组角膜创面愈合时间平均(5.47±1.33)d;B组平均(3.69±1.38)d,两组差异有统计学意义(t=9.0208;P<0.01).术后随访18~30个月,平均(22.5±5.6)个月,术后6月A、B组均无复发;A组术后6~12月复发3只眼;12~18月复发3只眼;18~24月后复发1只眼,复发率7.87%.B组术后6~18个月无复发,18~24月后复发2只眼,复发率1.96%,差异有统计学意义(U=8.802;P<0.01).结论 乙醇松解胬肉切除联合角膜缘上皮移植从复发基础和原因两方面抑制胬肉复发,值的临床推广应用.%Objective To observe clinical effect of alcohol lysis combined with corneal limbus epithelial transplantation in the treatment of primary pterygium.Mcthods The prospective randomized paired-eye trial was studied.The 176 patients (192 eyes) with primary pterygium were allocated to two groups: excision of pterygium with corneal limbus epithelial transplantation surgery (Group A, 87 cases of 90 eyes) and alcohol lysis combined with corneal limbus epithelial transplantation (Group B, 89 cases of 102 eyes).The average epithelial healing time of postoperative wound on cornea and recurrence rate between Group A and Group B were compared.Results The average epithelial healing time of postoperative was (5.47±1.33) days in Group A and (3.69±1.38) days in group B.The difference between two groups was significant (t=9.0208,P<0.001).Followed up periods of postoperative eyes ranged from 18-30 (22.5±5.6) months, 7 of 90 eyes (7.87%) in Group A and 2 of 102 eyes (1.96%) in Group B were recurred.The difference was significant (U=8.802, P<0

  2. Oral bullous eruption after taking lisinopril--case report and literature review.

    Science.gov (United States)

    Baričević, Marinka; Mravak Stipetić, Marinka; Situm, Mirna; Marinović, Branka; Seiwerth, Sven; Baričević, Denis; Lončar, Božana

    2013-07-01

    Drug-induced lesions of oral mucosa are well-established side effect of different commonly used drugs. A female patient under treatment for hypertension with an angiotensin-converting enzyme inhibitor (ACE inhibitor), lisinopril, developed blisters and ulcerations on oral mucosa 3 weeks after lisinopril intake. Due to clinical finding drug-induced pemphigus was considered. However, direct and indirect immunofluorescence anal-ysis revealed no autoantibodies that are commonly present in pemphigus while histological study suggested allergic reaction. Lisinopril was discontinued from further therapy and after a month after her first arrival patient has experienced complete remission of the disease. This case raises the question, whether the term pemphigus in drug-induced reactions could be used when immunopathological criteria for pemphigus are not fulfilled. PMID:23794071

  3. 结缔组织病诊疗进展%Therapy of Pemphigus and Bullous Pemphigoid

    Institute of Scientific and Technical Information of China (English)

    李明

    2007-01-01

    结缔组织病(Connective Tissue Disease,CTD)有广义和狭义之分,广义的结缔组织病是指体内结缔组织各种疾病的总称。狭义的结缔组织病是指由于免疫反应等引起的发生于疏松结缔组织的一类疾病。目前我们所指的结缔组织病系指狭义的结缔组织病,包括红斑狼疮(Lupus Erythematosus,LE)、皮肌炎/多肌炎(Dermatomyositis/Polymyositis,DM/PM)、硬皮病(Scleroderma)、类风湿性关节炎(Rheumatoid Arthritis,RA)、混合性结缔组织病(Mixed Connectijce Tissue Disease,MCTD)、干燥综合征(Siogren’s Syndrome,SS)及重叠综合征(Overlap Svndrome)等。

  4. Bullous eosinophilic cellulitis (Wells' syndrome) associated with Churg-Strauss syndrome

    NARCIS (Netherlands)

    Schuttelaar, M L A; Jonkman, M F

    2003-01-01

    We report a patient with Churg-Strauss syndrome (CSS) with asthma, eosinophilia, nasal polyposis and ANCA-associated multisystem vasculitis, who's skin eruption started with erythematous urticarial-plaques followed by haemorrhagic bullae. Histology of the plaques revealed 'flame figures' in the derm

  5. A Case of Bullous Skin Disease Presenting with Odynophagia: A Diagnostic Challenge

    Science.gov (United States)

    Kravvas, G.; Veitch, D.

    2016-01-01

    We report a case of Epidermolysis Bullosa Acquisita (EBA) that presented as a diagnostic challenge. A 60-year-old Qatari lady presented with odynophagia, oral ulceration, and weight loss. Multiple physicians investigated her for over 6 months with a multitude of tests and serial gastroscopies, all of which failed to reach a conclusive diagnosis. Only after referral to a dermatologist and full body examination was diagnosis finally achieved. After reviewing the literature, we provide a summary of EBA and highlight the importance of comprehensive clinical reviews in order to avoid unnecessary morbidity. PMID:27066279

  6. Chronic bullous disease of childhood--another cause for potential misdiagnosis of sexual abuse?

    OpenAIRE

    Coleman, H; Shrubb, V A

    1997-01-01

    There have been several reports of unusual skin conditions producing genital lesions that can be mistaken for sexual abuse. We report on another unusual skin condition, well known to dermatologists, that can again lead to potential misdiagnosis of sexual abuse.

  7. SKIN CARE AND WOUND MANAGEMENT IN PATIENTS WITH INHERITED BULLOUS EPIDERMOLYSIS

    OpenAIRE

    Murat-Sušić, Slobodna; Husar, Karmela; Marinović, Branka; Skerlev, Mihael; Štulhofer Buzina, Daška; Kljenak, Antun; Martinac, Ivana

    2014-01-01

    Inherited epidermolysis bullosa is a group of diseases characterized by skin/mucous membrane fragility and development of blisters and erosions after insignificant mechanical trauma. It is a multisystemic disease with complications occurring on numerous organs other than the skin. As there is no cure for these diseases, treatment consists of early recognition and therapy of complications, quality wound care and skin protection. Optimal wound treatment depends primarily on the type of the dise...

  8. Anaesthesia for Lung Volume Reduction Surgery in Bilateral Bullous Lung Disease: A Case Report

    Directory of Open Access Journals (Sweden)

    Raj Sahajanandan

    2008-01-01

    Full Text Available Lung Volume Reduction Surgery (LVRS has become an accepted modality for chronic pulmonary emphy-sema. Bilateral involve excision of emphysematous alveoli, which results in a 20% to 30% reduction in the volume of each lung. The goal of LVRS is to improve the respiratory mechanics in severe emphysema by re-expanding func-tional lung tissue that has been compressed by over-distended emphysematous alveoli, thus restoring diaphragmatic mobility and improving the bellows function of the chest wall structures. Anaesthesia for LVRS is a significant challenge to the anaesthetist as a result of high risk patient population and the nature of surgery. Management requires good understanding of the pathophysiology of the disease and surgical procedure. Close co-ordination be-tween the anaesthetist, surgeon and other support staff are of paramount importance.

  9. BULLOUS DEGENERATION OF THE LEFT LOWER LOBE IN A HEROIN-ADDICT

    NARCIS (Netherlands)

    SMEENK, FWJM; SERLIE, J; VANDERJAGT, EJ; POSTMUS, PE

    1990-01-01

    A 34 yr old heroin addict was referred because of chest pain caused by air-trapping in a bulla in the left lower lobe. There was a marked difference between the functional residual capacity measured by body-plethysmography and helium dilution. A slow wash-in and wash-out were demonstrated by ventila

  10. A Case Report of Ablepharon-Macrostomia Syndrome with Amniotic Membrane Grafting.

    Science.gov (United States)

    Feinstein, Eric; Traish, Aisha S; Aakalu, Vinay; Kassem, Iris S

    2015-01-01

    We describe a rare case of an infant who was born with multiple congenital anomalies, including the absence of eyelids. This patient had many dysmorphic features consistent with a severe phenotype of ablepharon-macrostomia syndrome (AMS) including a fish-like appearance of the mouth, rudimentary ears, absence of body hair, thin skin, absent nipples, abdominal distension, and genital abnormalities. Upon presentation, there was severe exposure keratopathy causing large bilateral sterile ulcers culminating in corneal melting of both eyes. An amniotic membrane graft was used to attempt to maintain the corneal surface integrity. However, because of the late presentation, the corneas could not be salvaged. Extensive surgical reconstruction of both eyelids and bilateral penetrating keratoplasty was ultimately performed successfully to protect the ocular surfaces while trying to maximize the visual potential. Early amniotic membrane grafting may be done at the bedside and may help preserve the ocular in patients with severe eyelid deformities until more definitive treatment is performed. PMID:26600791

  11. Indications for Corneal Transplantation at a Tertiary Referral Center in Tehran

    Science.gov (United States)

    Zare, Mohammad; Javadi, Mohammad-Ali; Einollahi, Bahram; Baradaran-Rafii, Alireza; Ghanavati, Siamak Zarei; Farsani, Mohammad-Reza Jamshidi; Mohammadi, Parviz; Feizi, Sepehr

    2010-01-01

    Purpose To report the indications and techniques of corneal transplantation at a tertiary referral center in Tehran over a 3-year period. Methods Records of patients who had undergone any kind of corneal transplantation at Labbafinejad Medical Center, Tehran, Iran from March 2004 to March 2007 were reviewed to determine the indications and types of corneal transplantation. Results During this period, 776 eyes of 756 patients (including 504 male subjects) with mean age of 41.3±21.3 years underwent corneal transplantation. The most common indication was keratoconus (n=317, 40.8%) followed by bullous keratopathy (n=90, 11.6%), non-herpetic corneal scars (n=62, 8.0%), infectious corneal ulcers (n=61, 7.9%), previously failed grafts (n=61, 7.9%), endothelial and stromal corneal dystrophies (n=28, 3.6%), and trachoma keratopathy (n=26, 3.3%). Other indications including Terrien’s marginal degeneration, post-LASIK keratectasia, trauma, chemical burns, and peripheral ulcerative keratitis constituted the rest of cases. Techniques of corneal transplantation included penetrating keratoplasty (n=607, 78.2%), deep anterior lamellar keratoplasty (n=108, 13.9%), conventional lamellar keratoplasty (n=44, 5.7%), automated lamellar therapeutic keratoplasty (n=8, 1.0%), and Descemet stripping endothelial keratoplasty (n=6, 0.8%) in descending order. The remaining cases were endothelial keratoplasty and sclerokeratoplasty. Conclusion In this study, keratoconus was the most common indication for penetrating keratoplasty which was the most prevalent technique of corneal transplantation. However, deep anterior lamellar keratoplasty is emerging as a growing alternative for corneal pathologies not involving the endothelium. PMID:22737335

  12. Indications for Corneal Transplantation at a Tertiary Referral Center in Tehran

    Directory of Open Access Journals (Sweden)

    Mohammad Zare

    2010-01-01

    Full Text Available Purpose: To report the indications and techniques of corneal transplantation at a tertiary referral center in Tehran over a 3-year period. Methods: Records of patients who had undergone any kind of corneal transplantation at Labbafinejad Medical Center, Tehran, Iran from March 2004 to March 2007 were reviewed to determine the indications and types of corneal transplantation. Results: During this period, 776 eyes of 756 patients (including 504 male subjects with mean age of 41.3±21.3 years underwent corneal transplantation. The most common indication was keratoconus (n=317, 40.8% followed by bullous keratopathy (n=90, 11.6%, non-herpetic corneal scars (n=62, 8.0%, infectious corneal ulcers (n=61, 7.9%, previously failed grafts (n=61, 7.9%, endothelial and stromal corneal dystrophies (n=28, 3.6%, and trachoma keratopathy (n=26, 3.3%. Other indications including Terrien′s marginal degeneration, post-LASIK keratectasia, trauma, chemical burns, and peripheral ulcerative keratitis constituted the rest of cases. Techniques of corneal transplantation included penetrating keratoplasty (n=607, 78.2%, deep anterior lamellar keratoplasty (n=108, 13.9%, conventional lamellar keratoplasty (n=44, 5.7%, automated lamellar therapeutic keratoplasty (n=8, 1.0%, and Descemet stripping endothelial keratoplasty (n=6, 0.8% in descending order. The remaining cases were endothelial keratoplasty and sclerokeratoplasty. Conclusion: In this study, keratoconus was the most common indication for penetrating keratoplasty which was the most prevalent technique of corneal transplantation. However, deep anterior lamellar keratoplasty is emerging as a growing alternative for corneal pathologies not involving the endothelium.

  13. The Role of Ultraviolet Radiation in the Ocular System of  Mammals

    Directory of Open Access Journals (Sweden)

    Mercede Majdi

    2014-10-01

    Full Text Available With decreasing levels of ozone in the atmosphere, we are being exposed to higher levels of ultraviolet radiation (UVR than ever before. UVR carries higher energy than visible light, and its effects on tissues include DNA damage, gene mutations, immunosuppression, oxidative stress and inflammatory responses. In the eye, UVR is strongly associated with the development of basal and squamous cell carcinoma of the eyelid, pterygium, photokeratitis, climatic droplet keratopathy, ocular surface squamous neoplasia, cataracts, and uveal melanoma, and is weakly associated with age-related macular degeneration. Despite overwhelming evidence regarding the deleterious effects on UVR, public health measures to encourage UV protection of the eyes is generally lacking. Options for photoprotection include sunglasses, wide brim hats, windshields, plastic films for side windows in cars, UV blocking contact lenses, and following the UV Index report daily. The American National Standards Institute currently has regulations regarding properties of UV blocking sunglasses; however, compliance in the US is not mandatory. On the other hand, UVR does have therapeutic applications in the eye, particularly, riboflavin activated by ultraviolet A light (UVA radiation is used clinically to slow the progression of keratoconus, post-LASIK keratectasia, and bullous keratopathy by crosslinking corneal collagen fibers. Additionally, riboflavin activated by UVA has been shown to have antibacterial, antiviral, and antiparasitic effects. This is clinically relevant in the treatment of infectious keratitis. Finally, exposure to low levels of light in the UV spectrum has been found to regulate the growth of the eye and lack of adequate exposure may increase the risk of development and progression of myopia.

  14. 去除上皮瓣的微型角膜刀法准分子激光角膜上皮瓣下磨镶术后早期兔角膜的修复方式研究%The early post-operative wound healing mode study of the rabbit cornea after flap-free epipolis laser in-situ keratomileusis

    Institute of Scientific and Technical Information of China (English)

    刘玺; 杜之渝; 向一旻; 黄正

    2009-01-01

    Objective To evaluate the wound healing mode of rabbit corneal epithelium after Epi-LASIK(epipolis laser in-situ keratomilensis ) and Flap-free Epi-LASIK (Flap-free epipolis laser in-situ keratomileusia).Methods Twenty-four New Zealand white rabbits(48 eyes) were randomly divided into four groups.There were six rabbits in every group, which were randomly treated with Epi-LASIK in one eye and Flap-free Epi-LASIK in the other by VISX20/20 excimer laser.Other three rabbits(6 eyes) without any treatment were used as control.The corneal epithelium wound healing mode was observed at 1,2,4,7 days after surgery by light microscope and electron microscope.The number of keratocytes cells in the central anterior stroma were counted by hematoxylin-cesin-stained.The expression of basic fibroblast growth factor (bFGF) in corneal stroma were assessed by Immunohistochemistry staining.Cornea stromal cell apoptosis was evaluated by terminal deoxyribonuc1eotidyl transferase-mediated deoxynuridine triphosphate nick end labeling (TUNEL) assay.All the data was analysised by t-test to evaluate the different of wound healing mode after Epi-LASIK and Flap-free Epi-LASIK.Results The findings of light microscope and electron microscope investigation demonstrated that, at the first day after surgery, there was one layer of neogenesis of the corneal epithelial cells covering the area of surgery in Flap-free Epi-LASIK specimens.It would increase gradually after 7 days.At the first day after the surgery, the base membrane of the corneal epithelium was tightly attached to the stroma in Epi-LASIK specimens.At the second day after the surgery, there was edema observed on the epithelium flap in the Epi-LASIK specimens.There were more keratocytes cells found at 4 days after surgery in Flap-free Epi-LASIK group (33.85±3.39) than that in Epi-LASIK group (t=-2.315,P<0.05).The expression of bFGF in Flap-free Epi-LASIK group at 2,4 days after surgery were 101.75±8.07 and 110.56±9.00, which were more

  15. Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation Efeitos da aplicação tópica da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar com implante de pericárdio de eqüino preservado em glicerina: avaliação clínica e morfológica

    Directory of Open Access Journals (Sweden)

    Roseli Borges Teixeira

    2004-02-01

    Full Text Available The effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye and the ointment base on the right eye (control eye twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after surgery. Vascularization was still evident on the 60th day, and looked similar in treated eyes and control eyes. The histologic evaluation showed a complete bilateral reepithelization and corneal vascularization three days after the surgery; intense vascularization in both eyes on days 15 and 30, that was much more pronounced in the treated cornea; and a bilateral predominance of polymorphonuclear cells until day 15, and mononuclear cells on day 30. Intact epithelium and stroma with new vessels, as well as graft absorption, in both eyes, were seen on the 60th day. The lamellar keratoplasty with equine pericardial graft was an effective model to study the inflammatory kinetics and corneal vascularization phenomenon. In this study, cyclosporine A did not inhibit corneal vascularization and it did not interfere in the corneal cicatricial process.Foram avaliados os efeitos da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar experimental com implante de pericárdio de equino. Dez cães foram divididos em grupos para estudo aos três, sete, 15, 30 e 60 dias de pós-operatório, recebendo implantes bilaterais e em seguida aplicação da pomada com ciclosporina A a 2% no olho

  16. "All-laser" endothelial corneal transplant in human patients

    Science.gov (United States)

    Rossi, Francesca; Menabuoni, Luca; Malandrini, Alex; Canovetti, Annalisa; Lenzetti, Ivo; Pini, Roberto

    2012-03-01

    Femtosecond laser sculpturing of corneal tissue is commonly used for the preparation of endothelial flaps. Diode laser welding of ocular tissues is a procedure that enables minimally invasive suturing of tissues. The combination of these laser based techniques results in a new approach to minimally invasive ophthalmic surgery, such as in endothelial corneal transplant (or endothelial keratoplasty - EK). In this work we present the "all laser" EK performed in human subjects. 24 pseudophakic patients with bullous keratopathy underwent EK: the femtosecond laser was used to prepare the 100 ìm thick and 8.5 mm diameter donor Descemet endothelial flap. After staining the stromal layer of the donor flap with a liquid ICG solution, the donor flap was inserted in the recipient eye by the use of the Busin injector. Then, the endothelial layer was laser-welded to the recipient eye (10 laser spots around the periphery of the flap), in order to reduce the risk of postoperative dislocation of the transplanted flap. A transplanted flap engraftment was observed in all the treated eyes. The staining procedure used to perform laser welding also enabled to evidence the stromal side of the donor flap, so as the flap was always placed in the right side position. The endothelial cells counts in both the laserwelded flaps and in a control group were in good agreement. The proposed technique is easy to perform and enables the reduction of postoperative endothelial flap dislocations.

  17. Diabetic and non-diabetic human cornea and tear γ-glutamyl transpeptidase activity

    OpenAIRE

    Langford, Marlyn

    2013-01-01

    Jordan M Burnham,1 Monali Sakhalkar,1 Marlyn P Langford,1 Chanping Liang,1 Thomas B Redens,1 Sushil K Jain21Department of Ophthalmology, 2Department of Pediatrics, Louisiana State University Health Sciences Center, Shreveport, LA, USABackground: Diabetes-related eye disease is due in part to oxidative stress. Gamma-glutamyl transpeptidase (GGT) is a γ-glutamyl cycle enzyme that protects against oxidative stress via glutathione recapture. This study investigates corneal and Schirmer ...

  18. Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea

    OpenAIRE

    Katsuo Tomoyose; Yuka Okada; Takayoshi Sumioka; Masayasu Miyajima; Flanders, Kathleen C.; Kumi Shirai; Tomoya Morii; Reinach, Peter S.; Osamu Yamanaka; Shizuya Saika

    2015-01-01

    To investigate the effects of loss of transient receptor potential vanilloid receptor 1 (TRPV1) on the development of neovascularization in corneal stroma in mice. Blocking TRPV1 receptor did not affect VEGF-dependent neovascularization in cell culture. Lacking TRPV1 inhibited neovascularization in corneal stroma following cauterization. Immunohistochemistry showed that immunoreactivity for active form of TGFβ1 and VEGF was detected in subepithelial stroma at the site of cauterization in both...

  19. Cornea stress test--evaluation of corneal endothelial function in vivo by contact lens induced stress

    Directory of Open Access Journals (Sweden)

    Saini Jagjit

    1997-01-01

    Full Text Available Reliable and valid assessment of corneal endothelial function is a critical input for diagnosing, prognosticating and monitoring progression of disorders affecting corneal endothelium. In 123 eyes, corneal endothelial function was assessed employing data from the corneal hydration recovery dynamics. Serial pachometric readings were recorded on Haag-Striet pachometer with Mishima-Hedbys modification before and after two hours of thick soft contact lens wear. Percentage Recovery Per Hour (PRPH was derived from raw data as an index of endothelial function. Assessed PRPH in pseudophakic corneal oedema and Fuchs′ endothelial dystrophy eyes (35.9 +/- 9.8% was significantly lower than normal controls (61.9 +/- 10.5%. On employing receiver operation characteristics curve analysis the tested results demonstrated high sensitivity (87% and specificity (92% for detection of low endothelial function at PRPH cut off of 47.5%. Using this PRPH cut off, 80% of Fuchs′ endothelial dystrophy and 93.3% of pseudophakic corneal oedema eyes could be demonstrated to have low endothelial function. A total of 66.7% of diabetic eyes also demonstrated PRPH of lower than 47.5%. Clear corneal grafts demonstrated PRPH values of 24.6% to 73.0%. Of 6 corneal grafts that demonstrated initial PRPH of lower than 47.5%, 4 failed within 4 to 6 months. Our data demonstrated high sensitivity and specificity of this corneal stress test. PRPH index was useful in quantifying endothelial function in clinical disorders including diabetes mellitus. The index PRPH was demonstrated to be useful in monitoring and prognosticating outcome of corneal grafts.

  20. Changes in aqueous humor following single or repeated UVB irradiation of rabbit cornea

    Czech Academy of Sciences Publication Activity Database

    Fris, M.; Čejková, Jitka; Midelfart, A.

    2007-01-01

    Roč. 245, č. 11 (2007), s. 1705-1711. ISSN 0721-832X R&D Projects: GA ČR GA304/06/1379 Institutional research plan: CEZ:AV0Z50390512 Keywords : Aqueous humour * Nuclear magnetic resonance * UVB rays Subject RIV: FF - HEENT, Dentistry Impact factor: 1.590, year: 2007

  1. Long-term results of cornea collagen cross-linking with riboflavin for keratoconus

    Directory of Open Access Journals (Sweden)

    Vinay Agrawal

    2013-01-01

    Full Text Available Corneal collagen cross-linking with riboflavin and UVA light (CXL is the only method designed to arrest the progression of keratoconus. Visual improvement generally starts 3 months after treatment. Reduction is coma seen on aberrometry in early postoperative phase is also responsible for the improvement in visual acuity. In the light of currently available data we can thus say that CXL is a safe procedure that is successful in arresting keratoconus.

  2. Iontophoretic Delivery of Riboflavin into the Rabbit Cornea:a Primary Study

    Institute of Scientific and Technical Information of China (English)

    Na Li; Xiujun Peng; Zhengjun Fan; Yu Xia

    2014-01-01

    Purpose:.To determine the penetrability of riboflavin into the corneal stroma by iontophoresis and to compare the perme-ability effects of different solvents. Methods:.Twenty rabbits were randomly divided into four groups:.a group that received 0.1% riboflavin-balanced salt solution (BSS) by iontophoresis, a group that received 0.1%riboflavin-saline solution by iontophoresis,.a group that re-ceived 0.1% riboflavin-distilled water solution by iontophore-sis, and a control group that received classical riboflavin in-stillation after corneal de-epithelialization..The degree of yel-lowing of the de-epithelialized corneal stromal button from each rabbit was compared. Results:.The yellow color scores for the corneal stromal but-tons in the three iontophoresis groups were compared with those of control group..Iontophoretic delivery of a 0.1% ri-boflavin-distilled water solution yielded similar yellow changes in the corneal stromal button when compared with classical ri-boflavin instillation after de-epithelialization..However,.the other two solvents did not sufficiently enhance the permeabili-ty of riboflavin. Conclusion:.Riboflavin can effectively penetrate into the corneal stroma to saturation levels by iontophoresis..Using distilled water as the solvent can promote penetrability.. (Eye Science 2014; 29:30-35).

  3. A Review of Collagen Cross-Linking in Cornea and Sclera

    Directory of Open Access Journals (Sweden)

    Xiao Zhang

    2015-01-01

    Full Text Available Riboflavin/UVA cross-linking is a technique introduced in the past decades for the treatment of keratoconus, keratectasia, and infectious keratitis. Its efficacy and safety have been investigated with clinical and laboratory studies since its first clinical application by Wollensak for the treatment of keratoconus. Although its complications are encountered during clinical practice, such as infection inducing risk, minimal invasion merits a further investigation on its future application in clinical practice. Recently, collagen cross-linking in sclera shows a promising prospect. In present study, we summarized the representative studies describing the clinical and laboratory application of collagen cross-linking published in past decades and provided our opinion on the positive and negative results of cross-linking in the treatment of ophthalmic disorders.

  4. Effect of two different UVA doses on the rabbit cornea and lens

    Czech Academy of Sciences Publication Activity Database

    Čejka, Čestmír; Pláteník, J.; Buchal, R.; Guryča, Vilém; Širc, Jakub; Vejražka, M.; Crkovská, J.; Ardan, Taras; Michálek, Jiří; Brůnová, B.; Čejková, Jitka

    2009-01-01

    Roč. 85, č. 3 (2009), s. 794-800. ISSN 0031-8655 R&D Projects: GA ČR GA304/06/1379; GA MŠk 1M0538 Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z40500505 Keywords : Metabolic profile * Induced Lipid -Peroxidation * Human skin fibroblasts Subject RIV: FF - HEENT, Dentistry Impact factor: 2.253, year: 2009

  5. Einfluss der clear-cornea-Phakoemulsifikation auf den Augeninnendruck bei Glaukompatienten - eine pro- und retrospektive Analyse

    OpenAIRE

    Schneider, Anja

    2008-01-01

    Anhand dieser Untersuchung sollte geklärt werden, welchen Einfluss eine extrakapsuläre Kataraktoperation mit clear-cornealer Schnitt-Technik, Phakoemulsifikation und Implantation einer Hinterkammerlinse auf den Augeninnendruck bei Patienten mit Offenwinkelglaukom hat. Dabei wurden sowohl retrospektiv die Daten von 76 Patienten als auch prospektiv die Daten von 49 Patienten aus der Universitätsaugenklinik Würzburg verglichen. Alle Patienten wiesen entweder ein Primäres Offenwinkelglaukom oder ...

  6. Pigment Deposition of Cosmetic Contact Lenses on the Cornea after Intense Pulsed-Light Treatment

    OpenAIRE

    Hong, Sojin; Lee, Jong Rak; Lim, Taehyung

    2010-01-01

    We report a case of corneal deposition of pigments from cosmetic contact lenses after intense pulsed-light (IPL) therapy. A 30-year-old female visited our outpatient clinic with ocular pain and epiphora in both eyes; these symptoms developed soon after she had undergone facial IPL treatment. She was wearing cosmetic contact lenses throughout the IPL procedure. At presentation, her uncorrected visual acuity was 2/20 in both eyes, and the slit-lamp examination revealed deposition of the color p...

  7. Keratitis Caused by Scedosporium apiospermum Successfully Treated with a Cornea Transplant and Voriconazole

    OpenAIRE

    Nulens, Eric; Eggink, Cathrien; Rijs, Antonius J. M. M.; Wesseling, Pieter; Verweij, Paul E.

    2003-01-01

    A case of Scedosporium apiospermum keratitis was successfully treated with oral voriconazole and penetrating keratoplasty. Voriconazole levels in the aqueous humor were 53% of the levels in plasma and exceeded the MIC for the isolate by sevenfold.

  8. Expression of Huntingtons disease markers in the cornea of minipig transgenic for the human mutated huntingtin

    Czech Academy of Sciences Publication Activity Database

    Ardan, Taras; Juhás, Štefan; Motlík, Jan

    2013-01-01

    Roč. 91, č. 252 (2013), S016-S016. ISSN 1755-375X. [European Association for Vision and Eye Research EVER 2013. 18.09.2013-21.09.2013, Nice] R&D Projects: GA MŠk ED2.1.00/03.0124; GA TA ČR(CZ) TA01011466 Institutional support: RVO:67985904 Keywords : huntingtin Subject RIV: EB - Genetics ; Molecular Biology

  9. Entwicklung eines humanen Cornea-Modells für die okulotoxische Sicherheitsprüfung

    OpenAIRE

    Manzer, Anna Katharina

    2010-01-01

    Currently, there is no regulatory accepted alternative method to fully replace the Draize rabbit eye irritation test on the living animal in risk assessment of chemicals, testing of pharmaceuticals and toxicological safety assessment of cosmetics. The toxicity of chemical substances with severe eye irritation potential can be evaluated by several validated in vitro assays (BCOP-assay, ICE-method) in the framework of a tiered testing strategy that is recommended by the OECD. Very mild irritant...

  10. An Update on Ocular Surface Epithelial Stem Cells: Cornea and Conjunctiva

    Science.gov (United States)

    Ramos, Tiago; Scott, Deborah; Ahmad, Sajjad

    2015-01-01

    The human ocular surface (front surface of the eye) is formed by two different types of epithelia: the corneal epithelium centrally and the conjunctival epithelium that surrounds this. These two epithelia are maintained by different stem cell populations (limbal stem cells for the corneal epithelium and the conjunctival epithelial stem cells). In this review, we provide an update on our understanding of these epithelia and their stem cells systems, including embryology, new markers, and controversy around the location of these stem cells. We also provide an update on the translation of this understanding into clinical applications for the treatment of debilitating ocular surface diseases. PMID:26146504

  11. MMP-8 Is Critical for Dexamethasone Therapy in Alkali-Burned Corneas Under Dry Eye Conditions.

    Science.gov (United States)

    Bian, Fang; Wang, Changjun; Tukler-Henriksson, Johanna; Pflugfelder, Stephen C; Camodeca, Caterina; Nuti, Elisa; Rossello, Armando; Li, De-Quan; de Paiva, Cintia S

    2016-11-01

    Our previous studies have shown that Dexamethasone (Dex) reduced the expression of matrix-metalloproteinases (MMPs -1,-3,-9,-13), IL-1β and IL-6, while it significantly increased MMP-8 mRNA transcripts in a concomitant dry eye and corneal alkali burn murine model (CM). To investigate if MMP-8 induction is responsible for some of the protective effects of Dex in CM, MMP-8 knock out mice (MMP-8KO) were subjected to the CM for 2 or 5 days and topically treated either with 2 μl of 0.1% Dexamethasone (Dex), or saline QID. A separate group of C57BL/6 mice were topically treated with Dex or BSS and received either 100 nM CAM12 (MMP-8 inhibitor) or vehicle IP, QD. Here we demonstrate that topical Dex treated MMP-8KO mice subjected to CM showed reduced corneal clarity, increased expression of inflammatory mediators (IL-6, CXCL1, and MMP-1 mRNA) and increased neutrophil infiltration at 2D and 5D compared to Dex treated WT mice. C57BL/6 mice topically treated with Dex and CAM12 IP recapitulated findings seen with MMP-8KO mice. These results suggest that some of the anti-inflammatory effects of Dex are mediated through increased MMP-8 expression. J. Cell. Physiol. 231: 2506-2516, 2016. © 2016 Wiley Periodicals, Inc. PMID:26923552

  12. The spatial organization of Descemet's membrane-associated type IV collagen in the avian cornea

    OpenAIRE

    1990-01-01

    The organization of type IV collagen in the unconventional basement membrane of the corneal endothelium (Descemet's membrane) was investigated in developing chicken embryos using anti-collagen mAbs. Both immunofluorescence histochemistry and immunoelectron microscopy were performed. In mature embryos (greater than 15 d of development), the type IV collagen of Descemet's membrane was present as an array of discrete aggregates of amorphous material at the interface between Descemet's membrane a...

  13. Evaluation of Surface anesthetic action of Aqueous Extract of Piper Betel leaf On Rabbit Cornea

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    Dr.T.Jayasree

    2014-07-01

    Full Text Available Aim: Piper betel Linn. (Piperaceae commonly known as betel leaf and the habit of betel chewing is widely prevalent in most parts of India. It is claimed to have aphrodisiac, laxative, antimicrobial, mucolytic, antiinflammatory and euphoric properties and proven antimutagenic and anti-carcinogenic effect. It is commonly observed that chewing of betel leaf produces numbness in the mouth, suggesting a possible local anesthetic effect. This observation prompted us to take this study . The aim of the study was to evaluate the local anesthetic activity of betel leaf extract after autoclaving the extract of betel leaf. Materials and methods: Extract of plain betel leaf, was tested for surface anesthetic activity using rabbits. Aqueous extraction of Piper betel leaf (AEPBL done by Soxhelts apparatus .Twenty male Rabbit’s were taken and divided in to four groups each group contain 5 animals, Group I - Standard (2% xylocaine, Group II - Test 0.3% (AEPBL, Group III - Test 0.6 % (AEPBL,Group IV - Test 12 % (AEPBL was instilled in conjunctival sac of right eyes. Standard protocol was followed to elicit light reflex, corneal reflex and to measure pupillary size after instilling the test drugs in the eye. There was dose dependent increase in onset and duration of local anesthetic activity with 6% and 12% doses of alcoholic extract of Piper betel leaf. Results: Betel leaf showed significant surface anesthetic activity comparable to that of Xylocaine. Conclusion: As a surface anesthetic, the onset was as quick as xylocaine and the duration was shorter than xylocaine

  14. The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva

    Czech Academy of Sciences Publication Activity Database

    Merjava, S.; Neuwirth, Aleš; Tanzerova, M.; Jirsová, K.

    2011-01-01

    Roč. 26, č. 3 (2011), s. 323-331. ISSN 0213-3911 Institutional research plan: CEZ:AV0Z50520514 Keywords : Posterior polymorphous dystrophy * Immunohostochemical analysis * Impression cytology Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.480, year: 2011

  15. Acute hydrops in the donor cornea graft in non-keratoconus patients

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    John B Cason

    2013-01-01

    Full Text Available A 44-year-old Hispanic male and 91-year-old Caucasian male presented to the clinic with acute vision loss and pain years after penetrating keratoplasty (PKP. Neither patient had a history of keratoconus. Both patients had a history of eye rubbing and intraocular device present in the anterior chamber. The first patient had a history of a glaucoma drainage tube and the second patient had an anterior chamber intraocular lens implanted. Anterior segment ocular coherence tomography showed deep stromal cystic cavities. Both patients exhibited breaks in the endothelium by ultrasound biomicroscopy and the histopathologic examination after repeat PKP. Those findings were most consistent with acute corneal hydrops in the donor graft.

  16. Incidence and complications of traditional eye medications in Nigeria in a teaching hospital

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    Ukponmwan Catherine

    2010-01-01

    Full Text Available Purpose: The aim of this study was to determine the types and nature of traditional eye medications (TEMs, their sources, and the ocular complications that may arise from use in a teaching hospital in Nigeria. Materials and Methods: A prospective study of consecutive subjects who used TEM before presentation to the Eye Clinic of the University of Benin Teaching Hospital, Benin City, Nigeria between July 1, 2004 and June 30, 2008. P < 0.05 was considered statistically significant. Results: A total of 113 subjects were evaluated of which 64 were males (56.6%, females (43.4% were females. There was no significant difference in the number of males and females (P > 0.05. Rural dwellers were more likely to use TEM than urban dwellers (P < 0.0001. The mean age of the subjects was 47.9 ΁ 22.3 years (range, 4-90 years. The most common traditional medication was derived from plant extracts (54.9% followed by concoctions (21.2%. Complications occurred in 54.8% of the subjects. Ocular complications included corneal opacities in 13.35% of subjects, staphyloma in 9%, and corneal ulcers in 8%. Other complications were panophthalmitis, endophthalmitis, uveitis, cataract, and bullous keratopathy. Eleven subjects underwent evisceration or enucleation of the affected eye. There was no significant difference in the type of medication used and ocular complications (P = 0.956. Sources of TEM were self-medication in 38.9% of subjects, relatives in 27.4%, and traditional healers in 17.7%. Conclusion: The use of TEM is a common practice that could be harmful and lead to blindness. Proper health education of the public and traditional healers can reduce the prevalence of preventable blindness.

  17. Long-term results of non-valved Glaucoma drainage implant surgery and glaucoma drainage implant combined with trabeculectomy

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    Teruhiko Hamanaka

    2014-01-01

    Full Text Available Purpose: The purpose was to investigate the efficacy and complications of nonvalved glaucoma drainage implant (GDI surgery and GDI combined with trabeculectomy. Subjects and Methods: Serial Japanese patients who received GDI were retrospectively investigated. The survival rate of eyes was analyzed using the Kaplan-Meier method, defining death as: (1 Intraocular pressure (IOP <6 mmHg, or ≥22 mmHg, and <20% reduction of preoperative IOP, (2 additional glaucoma surgery, (3 loss of light perception. Prognostic factors of age, sex, previous surgery, type of glaucoma, synechial closure, preoperative IOP, type of GDI (single-, double-plate Molteno, Baerveldt 350 and GDI combined with trabeculectomy were investigated employing proportional hazards analysis. Results: One hundred and twenty-four eyes of 109 patients aged 53.3 ± 7.8 years old were analyzed. Types of GDI were single-plate (15.3%, double-plate Molteno (71.8%, and Baerveldt 350 (12.9%. The results of survival rate analysis were 86.1, 71.1, 71.1, and 64.6% for 1, 3, 5, and 10 years respectively. Thirty-four eyes (27.4% died because of uncontrolled IOP (19.4%, loss of light perception (5.6%, and additional glaucoma surgery (2.4%. Single-plate Molteno was the only risk factor for failure. Persistent unphysiological hypotony (0.8% and bullous keratopathy (5.6% were observed. Conclusion: Nonvalved GDI surgery and GDI combined with trabeculectomy using nonabsorbable tube ligature proved to be an excellent device for any type of glaucoma in Japanese patients. Hypotony and corneal endothelial loss are the most serious complication in the long-term results of our patients.

  18. Exoresection via partial lamellar sclerouvectomy approach for uveal tumors: A successful performance by a novice surgeon

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    Rengin Aslıhan Kurt

    2010-01-01

    Full Text Available Rengin Aslıhan Kurt, Kaan GündüzDepartment of Ophthalmology, Ankara University Faculty of Medicine, Ankara, TurkeyPurpose: To evaluate the results and complications of exoresection via a partial lamellar sclerouvectomy (PLSU procedure for uveal tumors performed by a novice surgeon without help from an experienced surgeon.Methods: Medical records of 22 patients who underwent exoresection for uveal tumors between February 1999 and January 2009 were evaluated retrospectively. Exoresection was considered for tumors with their epicenter in the iris or in the ciliary body.Results: Twenty-two patients with a mean age of 45.9 years (range: 19–72 years were included in this study. The histopathologic diagnosis was uveal malignant melanoma in 16 patients, iridociliary nevus in 2 patients, iris nevus in 2 patients, and iridociliary melanocytoma in 2 patients. Postoperative complications included cataract in 11 patients (50%, scleral thinning in 4 patients (18%, vitreous hemorrhage in 2 patients (9%, hyphema in 2 patients (9%, secondary glaucoma in 2 patients (9%, iridodialysis in 1 patient (4.5%, bullous keratopathy in 1 patient (4.5%, and posterior synechiae in 1 patient (4.5%. At a mean follow-up of 40.1 months (range: 1–98 months, there were no recurrences or metastatic events.Conclusions: Exoresection seems to be an effective treatment option in selected cases of iridociliary and ciliary body tumors. Novice surgeons with limited training in PLSU should not discourage themselves from doing this type of surgery. Several postoperative complications inevitably occur and require frequent patient monitoring.Keywords: uveal melanoma, uveal nevus, iris, ciliary body, choroid, iris nevus, iris melanoma, ciliary nevus, ciliary melanoma, choroidal melanoma, ciliochoroidal melanoma, iridociliochoroidal melanoma, partial lamellar sclerouvectomy, exoresectionErratum for this paper has been published

  19. Ocular Effects of Sulfur Mustard

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    Yunes Panahi

    2013-06-01

    Full Text Available Purpose: To review current knowledge about ocular effects of sulfur mustard (SM and the associated histopathologic findings and clinical manifestationsMethods: Literature review of medical articles (human and animal studies was accomplished using PubMed, Scopus and ISI databases. A total of 274 relevant articles in English were retrieved and reviewed thoroughly.Results: Eyes are the most sensitive organs to local toxic effects of mustard gas. Ocular injuries are mediated through different toxic mechanisms including: biochemical damages, biomolecular and gene expression modification, induction of immunologic and inflammatory reactions, disturbing ultrastructural architecture of the cornea, and long-lasting corneal denervation. The resulting ocular injuries can roughly be categorized into acute or chronic complications. Most of the patients recover from acute injuries, but a minority of victims will suffer from chronic ocular complications. Mustard gas keratopathy (MGK is a devastating late complication of SM intoxication that proceeds from limbal stem cell deficiency (LSCD.Conclusion: SM induces several different damaging changes in case of ocular exposure; hence leading to a broad spectrum of ocular manifestations in terms of severity, timing and form. Unfortunately, no effective strategy has been introduced yet to inhibit or restore these damaging changes.

  20. Vogt-Koyanagi-Harada disease in a 9-year-old girl

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    Lott Pooi Wah

    2015-10-01

    Full Text Available Uveitis secondary to Vogt-Koyanagi-Harada disease is rare in children. To the best of our knowledge, this patient is the first reported case of uveitis attributed to VKH in a child in Malaysia. A 9-year-old girl complained of non-resolving painful red eyes bilaterally for 3 months. Anterior segment of right eye showed mutton fat keratic precipitates, posterior synechiae and moderate anterior chamber reaction. Anterior segment of left eye revealed mutton fat keratic precipitates, band keratopathy on peripheral 3 and 9 o' clock of the cornea, iris bombe, iris nodule and seclusio pupillae. Fundus examination of right eye revealed subretinal deposit at superior and inferior arcades with flat retina while left eye showed hazy view. Patient was then noted to have poliosis and vitiligo after 1 month from initial presentation. Mild cataract and widespread atrophy of the retinal pigment epithelium accounting for the loss in vision remained. This case report is to highlight the importance of early recognition of paediatric Vogt-Koyanagi-Harada and treating it aggressively to prevent the irreversible destructive sequalae of the disease.