WorldWideScience

Sample records for bulk tank milk

  1. Guidelines for monitoring bulk tank milk somatic cell and bacterial counts.

    Science.gov (United States)

    Jayarao, B M; Pillai, S R; Sawant, A A; Wolfgang, D R; Hegde, N V

    2004-10-01

    This study was conducted to establish guidelines for monitoring bulk tank milk somatic cell count and bacterial counts, and to understand the relationship between different bacterial groups that occur in bulk tank milk. One hundred twenty-six dairy farms in 14 counties of Pennsylvania participated, each providing one bulk tank milk sample every 15 d for 2 mo. The 4 bulk tank milk samples from each farm were examined for bulk tank somatic cell count and bacterial counts including standard plate count, preliminary incubation count, laboratory pasteurization count, coagulase-negative staphylococcal count, environmental streptococcal count, coliform count, and gram-negative noncoliform count. The milk samples were also examined for presence of Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma. The bacterial counts of 4 bulk tank milk samples examined over an 8-wk period were averaged and expressed as mean bacterial count per milliliter. The study revealed that an increase in the frequency of isolation of Staphylococcus aureus and Streptococcus agalactiae was significantly associated with an increased bulk tank somatic cell count. Paired correlation analysis showed that there was low correlation between different bacterial counts. Bulk tank milk with low (standard plate count also had a significantly low level of mean bulk tank somatic cell count (count (count (counts (count (count was less likely to be associated with somatic cell or other bacterial counts. Herd size and farm management practices had considerable influence on somatic cell and bacterial counts in bulk tank milk. Dairy herds that used automatic milking detachers, sand as bedding material, dip cups for teat dipping instead of spraying, and practiced pre-and postdipping had significantly lower bulk tank somatic cell and/or bacterial counts. In conclusion, categorized bulk tank somatic cell and bacterial counts could serve as indicators and facilitate monitoring of herd udder health and milk

  2. [Detection of Coxiella burnetii in dairy cattle bulk tank milk and single tank milk samples by confirmatory testing].

    Science.gov (United States)

    Hilbert, Angela; Andres, Tatjana; Werner, Ralf; Wehr, Roswitha; Fröhlich, Andreas; Conraths, Franz J; Henning, Klaus

    2015-01-01

    Q fever is a worldwide zoonotic disease caused by the pathogen Coxiella (C.) burnetii. A wide range of animal species is susceptible to this intracellular bacterium with great importance in ruminants. Human infections occur mainly by airborne transmission. C burnetii was detected in animal products such as raw milk, raw-milk cheese and butter prepared from raw milk as well as in the meat of infected animals. In cattle milk, the pathogen was detected up to 13 months after calving. The risk of human foodborne C. Burnetii infection is still considered to be low, but cannot be completely ruled out and remains under discussion. The aim of this study was to compare different laboratory diagnostic methods for C. burnetii in milk sample. The bulk tank and individual milk samples were sent and studied at the National Reference Laboratory for Q-fever in the context of confirmatory laboratory testing after clinical suspicion or retesting of previously antibody detection was in the analysis of 888 individual milk samples a match of 93.3% (Cohen-kappa). A total of 173 bulk milk samples and 2,807 individual milk samples from bovine herds for the presence of C. burnetii DNA and antibodies were tested against the pathogen. The pathogen was detected in 62.5% of the bulk milk samples and up to 60% in individual milk samples. The highest proportion of positive bulk milks was determined as 68.3% in 2012. In individual milk samples, the highest proportion of seropositive samples was 62.2%.

  3. Testing of bulk tank milk for Salmonella Dublin infection in Danish dairy herds

    DEFF Research Database (Denmark)

    Wedderkopp, A.; Stroger, U.; Bitsch, V.;

    2001-01-01

    of being test-negative in the third test round was 0.926 for a herd with 2 previous test-negative results. It was concluded that the investigated ELISA method was in general accordance with the cases of clinical S. Dublin infection recorded. and that the method has a potential for national screening......The usefulness of enzyme-linked immunosorbent assay (ELISA) was investigated as a simple method to screen for Salmonella Dublin infection in dairy herds, examining bulk tank milk samples for lipopolysaccharide (O:1,9,12) antibodies. The cut-off value for the ELISA on bulk tank milk was established...... based on individual milk samples (n = 2887) and bulk tank milk from 52 herds. Bulk tank milk samples (n = 5108) were collected from 1464 dairy herds located in 19 different areas. About 10% of the dairy herds in Denmark participated in the study. The percentage of herds changing from test...

  4. Risk factors affecting chemical and bacteriological quality of bulk tank milk in Kerman, Iran

    OpenAIRE

    Mansouri-Najand, Ladan; Rezaii, Zeinab

    2015-01-01

    Milk is often described as a complete food because it contains protein, sugar, fat, vitamins, and minerals. This study was performed to investigate risk factors affecting chemical and bacteriological quality of bulk tank milk. According to the following conducted experiments, the milk was divided into two standard and non-standard groups. Then, effect of risk factors on making the samples non-standard was studied. Risk factors such as type of milk delivery unit, distance of cattle farm from p...

  5. Helicobacteraceae in Bulk Tank Milk of Dairy Herds from Northern Italy

    OpenAIRE

    Valentina Bianchini; Camilla Recordati; Laura Borella; Valentina Gualdi; Eugenio Scanziani; Elisa Selvatico; Mario Luini

    2015-01-01

    Helicobacter pylori is responsible for gastritis and gastric adenocarcinoma in humans, but the routes of transmission of this bacterium have not been clearly defined. Few studies led to supposing that H. pylori could be transmitted through raw milk, and no one investigated the presence of other Helicobacteraceae in milk. In the current work, the presence of Helicobacteraceae was investigated in the bulk tank milk of dairy cattle herds located in northern Italy both by direct plating onto H. p...

  6. Effects of season, milking routine and cow cleanliness on bacterial and somatic cell counts of bulk tank milk.

    Science.gov (United States)

    Zucali, Maddalena; Bava, Luciana; Tamburini, Alberto; Brasca, Milena; Vanoni, Laura; Sandrucci, Anna

    2011-11-01

    The aim of the study was to investigate the effects of season, cow cleanliness and milking routine on bacterial and somatic cell counts of bulk tank milk. A total of 22 dairy farms in Lombardy (Italy) were visited three times in a year in different seasons. During each visit, samples of bulk tank milk were taken for bacterial and somatic cell counts; swabs from the teat surface of a group of cows were collected after teat cleaning and before milking. Cow cleanliness was assessed by scoring udder, flanks and legs of all milking cows using a 4-point scale system. Season affected cow cleanliness with a significantly higher percentage of non-clean (NC) cows during Cold compared with Mild season. Standard plate count (SPC), laboratory pasteurization count (LPC), coliform count (CC) and somatic cell count, expressed as linear score (LS), in milk significantly increased in Hot compared with Cold season. Coagulase-positive staphylococci on teat swabs showed higher counts in Cold season in comparison with the other ones. The effect of cow cleanliness was significant for SPC, psychrotrophic bacterial count (PBC), CC and Escherichia coli in bulk tank milk. Somatic cell count showed a relationship with udder hygiene score. Milking operation routine strongly affected bacterial counts and LS of bulk tank milk: farms that accomplished a comprehensive milking scheme including two or more operations among forestripping, pre-dipping and post-dipping had lower teat contamination and lower milk SPC, PBC, LPC, CC and LS than farms that did not carry out any operation.

  7. Stochastic simulation modeling to determine time to detect Bovine Viral Diarrhea antibodies in bulk tank milk

    DEFF Research Database (Denmark)

    Foddai, Alessandro; Enøe, Claes; Krogh, Kaspar;

    2014-01-01

    A stochastic simulation model was developed to estimate the time from introduction ofBovine Viral Diarrhea Virus (BVDV) in a herd to detection of antibodies in bulk tank milk(BTM) samples using three ELISAs. We assumed that antibodies could be detected, after afixed threshold prevalence...

  8. Effect of cleaning procedure and hygienic condition of milking equipment on bacterial count of bulk tank milk.

    Science.gov (United States)

    Bava, Luciana; Zucali, Maddalena; Sandrucci, Anna; Brasca, Milena; Vanoni, Laura; Zanini, Lucio; Tamburini, Alberto

    2011-05-01

    The aim of the study was to describe the characteristics of cleaning procedures for milking equipment applied in intensive dairy farms in Lombardy (Italy) and to study their relationships with bacterial count of bulk milk and hygienic condition of milking machine components. A group of 22 dairy farms was visited twice (winter and summer) in order to collect bulk tank milk and post-rinse water samples and swabs from liners and milk receiver. Samples were analysed to determine: standard plate count (SPC), laboratory pasteurization count (LPC), psychrotrophic bacteria count (PBC), coliform count (CC) and Escherichia coli. Cleaning procedures were monitored using electronic milk flow meters with specific software for the measurement of the duration of each cleaning phase, circulating solution temperature and electrical conductivity, turbulence and water filling percentage of pipelines. The results showed that farms classified as high and low milk total bacteria count significantly differed both in terms of liners and receiver bacterial contamination and in terms of water temperature reached during the detergent phase of cleaning milking equipment. Significant positive correlations were found among total bacteria count in milk and bacterial contamination of the liners. Maximum water temperature reached during the cleaning cycle of milking equipment was very low (34.4±8.9°C on average); most of the observations (88.6%) corresponded to water temperatures count of milk and post-rinse water and coliform count in liners. Routine check and regulation of water temperature during the washing phase of the milking machine can be a simple and effective way to control one of the main risk factors for bacteriological quality of bulk tank milk.

  9. Identification and characterization of elevated microbial counts in bulk tank raw milk.

    Science.gov (United States)

    Hayes, M C; Ralyea, R D; Murphy, S C; Carey, N R; Scarlett, J M; Boor, K J

    2001-01-01

    The bacterial composition of bulk tank milk from 13 farms was examined over a 2-wk period to characterize sudden elevations in the total bacterial count referred to as "spikes." Bulk tank milk samples collected at each pick-up were analyzed for standard plate count, Petrifilm aerobic count, somatic cell count, gram-negative organisms, and streptococci. Twenty standard plate count spikes were observed: 12 associated with streptococci, 4 associated with gram-negative organisms, 2 associated with streptococci and gram-negative organisms, and 2 that were not definitively characterized. Spikes ranged from 14,000 to 600,000 cfu/ml. Streptococcus uberis was isolated as the predominant organism from 11 spikes, and Escherichia coli was isolated from 4 spikes. Statistical analysis of total bacterial counts indicated a high correlation (r = 0.94) between standard plate counts and Petrifilm aerobic count. Regression analysis of standard plate counts and Petrifilm aerobic counts yielded the equation log10 (standard plate count) = 0.73 + 0.85log10 (Petrifilm aerobic count), indicating that the correlation, although strong, is not one to one. In a related pilot study, triplicate bulk tank milk samples were collected and analyzed for total bacterial count and presumptive streptococcus, gram-negative, and staphylococcus counts. Two-way ANOVA of these triplicate data indicated a lack of significant variation among the triplicate samples, suggesting that one sample can reliably gauge the microbial status of the entire bulk tank.

  10. Short communication: Evaluation of bulk tank milk microbiological quality of nine dairy farms in Tennessee.

    Science.gov (United States)

    Gillespie, B E; Lewis, M J; Boonyayatra, S; Maxwell, M L; Saxton, A; Oliver, S P; Almeida, R A

    2012-08-01

    The purpose of this study was to evaluate the bulk tank milk (BTM) quality of 9 East Tennessee dairy farms and to determine its relationship with selected quality milk parameters. Bulk tank milk samples (n=1,141) were collected over a 42-mo period (June 2006 through November 2009) from farms, based on their preliminary incubation count (PIC) history. Parameters of BTM quality evaluated in this study included somatic cell count (SCC), standard plate count (SPC), PIC, laboratory pasteurization count (LPC), Staphylococcus spp. count, Streptococcus spp. count, and coliform count. Strong correlations between SPC and Streptococcus spp. counts (0.72) and between SPC and PIC (0.70) were found. However, moderate correlations were seen among other milk quality parameters. In addition, seasonal variations for some milk quality parameters were noted. For example, milk quality parameters such as SCC, SPC, LPC, and coliform count were significantly higher in summer, whereas Streptococcus spp. counts were significantly higher in winter. No seasonal variation in PIC or Staphylococcus spp. counts was observed. Summarizing, results from this investigation showed the importance of using several bacterial counts (SCC, SPC, PIC, LPC, Streptococcus spp. count, Staphylococcus spp. count, and coliform counts) as simultaneous indicators of milk quality.

  11. Phytoestrogens and their metabolites in bulk-tank milk: effects of farm management and season.

    Science.gov (United States)

    Adler, Steffen A; Purup, Stig; Hansen-Møller, Jens; Thuen, Erling; Steinshamn, Håvard

    2015-01-01

    Phytoestrogens have structures similar to endogenous steroids and may induce or inhibit the response of hormone receptors. The objectives of the present study were to compare the effects of long-term vs. short-term grassland management in organic and conventional dairy production systems, compare organic and conventional production systems and assess seasonal variation on phytoestrogen concentrations in bulk-tank milk. The concentrations of phytoestrogens were analyzed in bulk-tank milk sampled three times in two subsequent years from 28 dairy farms: Fourteen organic (ORG) dairy farms with either short-term or long-term grassland management were paired with 14 conventional (CON) farms with respect to grassland management. Grassland management varied in terms of time since establishment. Short-term grassland management (SG) was defined as establishment or reseeding every fourth year or more often, and long-term grassland management (LG) was defined as less frequent establishment or reseeding. The proportion of red clover (Trifolium pretense L.) in the herbage was positively correlated with milk concentrations of the mammalian isoflavone equol. Therefore, organically produced bulk-tank milk contained more equol than conventionally produced milk, and milk from ORG-SG farms had more equol than milk from ORG-LG farms. Milk produced during the indoor-feeding periods had more equol than milk produced during the outdoor feeding period, because pastures contained less red clover than fields intended for silage production. Organically produced milk had also higher concentrations of the mammalian lignan enterolactone, but in contrast to equol, concentrations increased in the outdoor-feeding periods compared to the indoor-feeding periods. There were no indications of fertility problems on ORG-SG farms who had the highest red clover proportions in the herbage. This study shows that production system, grassland management, and season affect milk concentrations of phytoestrogens

  12. Evaluation of milk yield losses associated with Salmonella antibodies in bulk tank milk in bovine dairy herds

    DEFF Research Database (Denmark)

    Nielsen, Torben Dahl; Green, L. E.; Kudahl, Anne Margrethe Braad;

    2012-01-01

    The effect of Salmonella on milk production is not well established in cattle. The objective of this study was to investigate whether introduction of Salmonella into dairy cattle herds was associated with reduced milk yield and determine the duration of any such effect. Longitudinal data from 2005...... through 2009 were used, with data from 12 mo before until 18 mo after the estimated date of infection. Twenty-eight case herds were selected based on an increase in the level of Salmonella-specific antibodies in bulk-tank milk from ... was randomly allocated to the control herds. Hierarchical mixed effect models with the outcome test-day yield of energy-corrected milk (ECM)/cow were used to investigate daily milk yield before and after the estimated herd infection date for cows in parities 1, 2, and 3+. Control herds were used to evaluate...

  13. Helicobacteraceae in Bulk Tank Milk of Dairy Herds from Northern Italy.

    Science.gov (United States)

    Bianchini, Valentina; Recordati, Camilla; Borella, Laura; Gualdi, Valentina; Scanziani, Eugenio; Selvatico, Elisa; Luini, Mario

    2015-01-01

    Helicobacter pylori is responsible for gastritis and gastric adenocarcinoma in humans, but the routes of transmission of this bacterium have not been clearly defined. Few studies led to supposing that H. pylori could be transmitted through raw milk, and no one investigated the presence of other Helicobacteraceae in milk. In the current work, the presence of Helicobacteraceae was investigated in the bulk tank milk of dairy cattle herds located in northern Italy both by direct plating onto H. pylori selective medium and by screening PCR for Helicobacteraceae, followed by specific PCRs for H. pylori, Wolinella spp., and "Candidatus Helicobacter bovis." Three out of 163 bulk milk samples tested positive for Helicobacteraceae, but not for the subsequent PCRs. H. pylori was not isolated in any case. However, given similar growth conditions, Arcobacter butzleri, A. cryaerophilus, and A. skirrowii were recovered. In conclusion, the prevalence of Helicobacteraceae in raw milk was negligible (1.8%), and H. pylori was not identified in any of the positive samples, suggesting that, at least in the farming conditions of the investigated area, bovine milk does not represent a potential source of infection. PMID:26090429

  14. Helicobacteraceae in Bulk Tank Milk of Dairy Herds from Northern Italy

    Directory of Open Access Journals (Sweden)

    Valentina Bianchini

    2015-01-01

    Full Text Available Helicobacter pylori is responsible for gastritis and gastric adenocarcinoma in humans, but the routes of transmission of this bacterium have not been clearly defined. Few studies led to supposing that H. pylori could be transmitted through raw milk, and no one investigated the presence of other Helicobacteraceae in milk. In the current work, the presence of Helicobacteraceae was investigated in the bulk tank milk of dairy cattle herds located in northern Italy both by direct plating onto H. pylori selective medium and by screening PCR for Helicobacteraceae, followed by specific PCRs for H. pylori, Wolinella spp., and “Candidatus Helicobacter bovis.” Three out of 163 bulk milk samples tested positive for Helicobacteraceae, but not for the subsequent PCRs. H. pylori was not isolated in any case. However, given similar growth conditions, Arcobacter butzleri, A. cryaerophilus, and A. skirrowii were recovered. In conclusion, the prevalence of Helicobacteraceae in raw milk was negligible (1.8%, and H. pylori was not identified in any of the positive samples, suggesting that, at least in the farming conditions of the investigated area, bovine milk does not represent a potential source of infection.

  15. Spore populations among bulk tank raw milk and dairy powders are significantly different.

    Science.gov (United States)

    Miller, Rachel A; Kent, David J; Watterson, Matthew J; Boor, Kathryn J; Martin, Nicole H; Wiedmann, Martin

    2015-12-01

    To accommodate stringent spore limits mandated for the export of dairy powders, a more thorough understanding of the spore species present will be necessary to develop prospective strategies to identify and reduce sources (i.e., raw materials or in-plant) of contamination. We characterized 1,523 spore isolates obtained from bulk tank raw milk (n=33 farms) and samples collected from 4 different dairy powder-processing plants producing acid whey, nonfat dry milk, sweet whey, or whey protein concentrate 80. The spores isolated comprised 12 genera, at least 44 species, and 216 rpoB allelic types. Bacillus and Geobacillus represented the most commonly isolated spore genera (approximately 68.9 and 12.1%, respectively, of all spore isolates). Whereas Bacillus licheniformis was isolated from samples collected from all plants and farms, Geobacillus spp. were isolated from samples from 3 out of 4 plants and just 1 out of 33 farms. We found significant differences between the spore population isolated from bulk tank raw milk and those isolated from dairy powder plant samples, except samples from the plant producing acid whey. A comparison of spore species isolated from raw materials and finished powders showed that although certain species, such as B. licheniformis, were found in both raw and finished product samples, other species, such as Geobacillus spp. and Anoxybacillus spp., were more frequently isolated from finished powders. Importantly, we found that 8 out of 12 genera were isolated from at least 2 different spore count methods, suggesting that some spore count methods may provide redundant information if used in parallel. Together, our results suggest that (1) Bacillus and Geobacillus are the predominant spore contaminants in a variety of dairy powders, implying that future research efforts targeted at elucidating approaches to reduce levels of spores in dairy powders should focus on controlling levels of spore isolates from these genera; and (2) the spore

  16. Risk factors for Coxiella burnetii antibodies in bulk tank milk from Danish dairy herds

    DEFF Research Database (Denmark)

    Agger, Jens Frederik; Paul, Suman; Christoffersen, Anna-Bodil;

    2013-01-01

    The aim was to identify risk factors associated with Coxiella burnetii antibody positivity in bulk tank milk (BTM) samples from 100 randomly selected Danish dairy cattle herds. Antibody levels were measured by an enzyme-linked immuno-sorbent assay. Before testing the herds, the farm managers were...... interviewed about hired labour, biosecurity, housing and herd health during the 12 months prior to the study. Variables considered important for C. burnetii antibody positivity in multivariable logistic regression analysis included the sharing of machines between farms (OR = 3.6), human contacts (OR = 4.......2), artificial insemination by other people than artificial insemination technicians (OR = 7.7), routine herd health contract with the veterinarian (OR = 4.3) and hygiene precautions taken by veterinarians (OR = 5). In addition, herd size, hired labour, trading of cattle between farms, quarantine and use...

  17. Prevalence of Bovine Mastitis Pathogens in Bulk Tank Milk in China

    Science.gov (United States)

    Wang, Ya Jing; Qin, Yun; Guix Vallverdú, Roger; Maldonado García, Jaime; Sun, Wei; Li, Shengli; Cao, Zhijun

    2016-01-01

    The objectives of this study were to estimate the herd prevalence of major mastitis pathogens in bulk tank milk (BTM) in China dairy herds, to determine the relationship between the presence of mastitis pathogens and bulk tank milk somatic cell counts (BTSCC), and to investigate the impact of different dairy cattle farming modes and region on bacterial species. BTM samples collected from 894 dairy herds in China were examined for the presence of mastitis pathogens. The Flinders Technology Associates (FTA) cards were used for BTM sample collection, storage, and transportation and bacterial DNA amplification by real-time PCR. Among contagious pathogens, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae were detected in 50.1, 92.2, and 72.3% of the 894 BTM samples, respectively. Among environmental pathogens, E. coli, Streptococcus uberis, Enterococcus spp., Klebsiella spp., Serratia marcescens, Corynebacterium bovis, and Arcanobacterium pyogenes were detected in 28.6, 8.9, 35.7, 20.0, 1.3, 17.0, and 67.2% of the BTM samples, respectively. Staphylococcal β-lactamase gene was detected in 61.7% of the BTM samples. The presence of Staphylococcus aureus and Arcanobacterium pyogenes were significantly associated with high BTSCC, respectively. Significant differences were found in presence of Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae in BTM sampled from the small household farms, dairy-farming communities, and large-scaled dairy farms. There were significant differences in the presence of Streptococcus agalactiae, Streptococcus dysgalactiae, Arcanobacterium pyogenes, staphylococcal β-lactamase gene, Staphylococcus spp., Klebsiella spp., Enterococcus spp., and Streptococcus uberis in BTM among Inner Mongolia, Heilongjiang, and Hebei province. In conclusion, contagious mammary pathogens are predominated among pathogens in BTM samples in China. PMID:27187065

  18. Prevalence, antimicrobial resistance, and molecular characterization of methicillin-resistant Staphylococcus aureus from bulk tank milk of dairy herds.

    Science.gov (United States)

    Kreausukon, K; Fetsch, A; Kraushaar, B; Alt, K; Müller, K; Krömker, V; Zessin, K-H; Käsbohrer, A; Tenhagen, B-A

    2012-08-01

    It was the objective of the study to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in bulk tank milk from German dairy herds and to characterize isolates from bulk tank milk with respect to their Staph. aureus protein A (spa) and staphylococcal cassette chromosome mec (SCCmec) type, their phenotypic antimicrobial resistance and resistance- resp. virulence-associated genes using broth microdilution and a microarray for Staph. aureus. Bulk tank milk samples (25 mL) were tested for MRSA using a 2-step selective enrichment protocol. Presumptive MRSA were confirmed by PCR. Thirty-six isolates collected from bulk tank milk of dairy herds in 2009 and 2010 were included in the characterization. All isolates displayed spa-types assigned to the clonal complex CC398. Based on the epidemiological cut-off values for the interpretation of minimum inhibitory concentrations isolates were resistant to tetracycline (100%), clindamycin (58%), erythromycin (52%), quinupristin/dalfopristin (36%), and kanamycin (27%). Isolates did not carry genes associated with typical virulence factors for Staph. aureus such as the Panton-Valentine leukocidin. However, they did carry hemolysin genes. Livestock-associated MRSA of CC398 does occur in German dairy herds and the strains have similar properties as described for strains from pigs. PMID:22818451

  19. Occurrence and antimicrobial resistance of Staphylococcus aureus in bulk tank milk and milk filters

    Directory of Open Access Journals (Sweden)

    Kateřina Bogdanovičová

    2014-02-01

    Full Text Available This work is focused on the monitoring of Staphylococcus aureus prevalence in raw milk and milk filters, its antibiotic resistance and detection of methicillin resistant Staphylococcus aureus (MRSA. Samples of raw cow´s milk and milk filters were collected in the period from 2012 till 2014, from 50 dairy farms in the Czech Republic. The total of 261 samples (164 samples of raw milk and 97 milk filters were cultivated on Baird-Parker agar. Both the typical and atypical colonies were examined by plasmacoagulase test and PCR method was used for detection of species specific fragment SA442 and mecA gene. Standard disk diffusion method was used to determinate resistance to antimicrobial agents. The bacterium Staphylococcus aureus was detected on 25 farms (50%. The antimicrobial resistance showed differences between the farms. Total of 58 samples were positive for Staphylococcus aureus, of which were 37 (14.2% isolated from raw milk samples and 21 (8.1% from milk filters. From these samples we isolated 62 Staphylococcus aureus strains, 41 isolates bacteria S. aureus from raw milk (66.1% and 21 isolates S. aureus from milk filters (33.9%. The presence of antibiotic resistance in Staphylococcus aureus isolates was low, most of them were resistant to amoxicilin. According to the results obtained by the PCR method for the methicillin - resistant S. aureus (MRSA, the mecA gene was present in 6 strains (9.7%, 4 isolates obtained from milk samples (6.5% and 2 isolates from milk filters (3.2%.  These isolates can be considered as a possible source of resistance genes, which can be spread through the food chain. Nowadays, a globally unfavourable increasing trend of prevalence of methicillin resistant staphylococci strains especially Staphylococcus aureus is being observed worldwide. The improper hygiene and poor farm management practices contributed to the presence of S. aureus in the milk. This may have contributed to the high level of S. aureus isolated

  20. Effect of short-term versus long-term grassland management and seasonal variation in organic and conventional dairy farming on the composition of bulk tank milk

    DEFF Research Database (Denmark)

    Adler, S A; Jensen, Søren Krogh; Govasmark, E;

    2013-01-01

    Bulk tank milk from 28 dairy farms was sampled every second month for 2 yr to assess the effects of grassland management, production system and season on milk fatty acid (FA) composition, concentrations of fat-soluble vitamins, Se, and milk sensory quality. Grassland management varied in terms...... feeding periods had potential health benefits due to FA composition. In contrast, the higher milk-fat proportions of saturated FA in milk from ORG farms may be perceived as negative for human health....

  1. Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

    DEFF Research Database (Denmark)

    Angen, Øystein; Ståhl, Marie; Agerholm, J. S.;

    2011-01-01

    Milk samples of 12 Danish dairy herds were collected 3 times during an 11-mo period and tested for Coxiella burnetii DNA by real-time PCR, detecting the IS1111 element, and for the presence of antibodies against the bacterium by ELISA. On average, 25% of 1,514 samples were seropositive and 32% were...... positive for C. burnetii DNA. Among the 485 DNA-positive samples, quantification cycle values ranging from 15.8 to 37.8 were found. Test sensitivity did not increase after DNA extraction from the cream fraction compared with full milk. The relationship between antibody levels and bacterial shedding...... protein concentration in milk. The antibody levels in bulk tank milk and prevalence levels of C. burnetii DNA and antibodies in individual cow milk samples were correlated. A significant correlation was also found between the quantification cycle values of the cow samples (weighted according to milk yield...

  2. Latent class analysis of bulk tank milk PCR and ELISA testing for herd level diagnosis of Mycoplasma bovis

    DEFF Research Database (Denmark)

    Nielsen, Per Kantsø; Petersen, Mette Bisgaard; Nielsen, Liza Rosenbaum;

    2015-01-01

    The bacterium Mycoplasma bovis causes disease in cattle of all ages. An apparent increase in the occurrence of M. bovis associated outbreaks among Danish dairy cattle herds since 2011 has prompted a need for knowledge regarding herd-level diagnostic performance. Therefore, the objective...... of this study was to evaluate the herd-level diagnostic performance of an indirect ELISA test by comparison to a real-time PCR test when diagnosing M. bovis in cattle herds of bulk tank milk. Bulk tank milk samples from Danish dairy herds (N=3437) were analysed with both the antibody detecting BIO K 302 M....... bovis ELISA kit and the antigen detecting PathoProof Mastitis Major-3 kit. As none of these are considered a gold standard test for herd-level diagnostics we applied a series of Bayesian latent class analyses for a range of ELISA cut-off values. The negative and positive predictive values were...

  3. Fatty acid content, vitamins and selenium in bulk tank milk from organic and conventional Swedish dairy herds during the indoor season

    OpenAIRE

    Fall, Nils; Emanuelson, Ulf

    2011-01-01

    Fatty acids, vitamins and minerals in milk are important for the human consumer, the calf and the cow. Studies indicate that milk from organic and conventional dairy herds may differ in these aspects. The aim of this study was therefore to investigate whether there are differences in the fatty acid composition and concentration of vitamins and selenium in milk between organic and conventional herds in Sweden. Bulk tank milk was sampled in 18 organic and 19 conventional dairy herds on three oc...

  4. Molecular detection of Streptococcus agalactiae in bovine raw milk samples obtained directly from bulk tanks.

    Science.gov (United States)

    Elias, Acácia Orieth; Cortez, Adriana; Brandão, Paulo Eduardo; da Silva, Rodrigo Costa; Langoni, Helio

    2012-08-01

    Mastitis is the most common infectious disease affecting dairy cattle; in addition, it remains the most economically important disease of dairy industries around the world. Streptococcus agalactiae, a contagious pathogen associated with subclinical mastitis, is highly infectious. This bacterium can cause an increase in bulk tank bacterial counts (BTBC) and bulk tank somatic cell counts (BTSCC). The microbiological identification of S. agalactiae in samples from bulk tanks is an auxiliary method to control contagious mastitis. Thus, there are some limitations for time-consuming cultures or identification methods and additional concerns about the conservation and transport of samples. Bulk tank samples from 247 dairy farms were cultured and compared through polymerase chain reaction (PCR), directed to 16S rRNA genes of S. agalactiae, followed by BTBC and S. agalactiae isolation. The mean value of BTBC was 1.08×10(6) CFU mL(-1) and the bacterium was identified through the microbiological method in 98 (39.7%; CI(95%)=33.8-45.9%) and through PCR in 110 (44.5%; CI(95%)=38.5-50.8%) samples. Results indicated sensitivity of 0.8571±0.0353 (CI(95%)=0.7719-0.9196) and specificity of 0.8255±0.0311 (CI(95%)=0.7549-0.8827). The lack of significant difference between microbiological and molecular results (κ=0.6686±0.0477 and CI(95%)=0.5752-0.7620) indicated substantial agreement between the methods. This suggests that PCR can be used for bulk tank samples to detect contagious mastitis caused by S. agalactiae.

  5. Microbiological Examination of Bulk Tank Goat's Milk in the Castilla y León Region in Northern Spain.

    Science.gov (United States)

    Álvarez-Suárez, María-Elena; Otero, Andrés; García-López, María-Luisa; Santos, Jesús A

    2015-12-01

    The purpose of the study was to evaluate the microbiological status (mesophilic aerobic microorganism counts) of 68 samples of bulk tank goat's milk and determine the risk associated with the foodborne pathogens Staphylococcus aureus, enteropathogenic and Shiga toxin-producing Escherichia coli, and Cronobacter sakazakii. Most samples (83.8%) complied with the limits of mesophilic aerobe counts set in the European Union for milk of species other than cows. A total of 144 isolates of coagulase-positive staphylococci were characterized, and 11 (7.6%) of them carried staphylococcal enterotoxin (SE) genes of the classical types (encoding SEA to SEE), distributed as follows: 4 carried the SEA gene, 1 the SEB gene, and 6 the SED gene. C. sakazakii was not detected in any sample. Regarding detection of E. coli virulence-related genes in enriched milk samples, 12 milk samples were positive only for the presence of stx genes, 4 were positive for both stx and eae genes, and 20 were negative for stx amplification and positive for eae amplification. Seven enteropathogenic E. coli and 9 Shiga toxin-producing E. coli isolates (one of them of serogroup O157) were recovered. In conclusion, goat's milk produced on farms in Castilla y León is generally in accordance with European Union standards, but the presence of pathogenic E. coli isolates indicates that the consumption of raw goat's milk may pose a risk to public health.

  6. Modeling the effect of direct and indirect contamination of on-farm bulk tank milk with Mycobacterium avium subsp. paratuberculosis

    DEFF Research Database (Denmark)

    Okura, Hisako; Nielsen, Søren Saxmose; Toft, Nils

    2013-01-01

    in farm bulk tank milk and simulating the effect of direct and indirect contamination with MAP. The effect of discarding milk from test-positive cows at different prevalences was assessed. The concentration of MAP in milk was estimated using a simulation model, while taking direct and indirect...... milk from test positive cows would result in discarding 11% of milk and reduce the MAP level by 80%. Due to poor sensitivity of the diagnostic test, removing test-positive cows would not further reduce the already low concentration of MAP and it would not guarantee the milk as MAP-free. The model was...

  7. Salmonella dublin in Danish dairy herds: Frequency of change to positive serological status in bulk tank milk ELISA in relation to serostatus of neighbouring farms

    DEFF Research Database (Denmark)

    Wedderkopp, A.; Strøger, U.; Lind, Peter

    2001-01-01

    Bulk tank milk from 1,429 herds were collected in 3 rounds from 19 different geographic areas. The milk samples were tested by use of indirect LPS-ELISA procedure to detect Salmonella dublin antibodies. From the obtained OD-values herd seroprevalence in the given area was determined and GR...

  8. Prevalence, antimicrobial susceptibility and molecular typing of Methicillin-Resistant Staphylococcus aureus (MRSA) in bulk tank milk from southern Italy.

    Science.gov (United States)

    Parisi, A; Caruso, M; Normanno, G; Latorre, L; Sottili, R; Miccolupo, A; Fraccalvieri, R; Santagada, G

    2016-09-01

    This paper assesses the prevalence of MRSA in bulk tank milk (BTM) samples from southern Italy, and the relationship between the Coagulase Positive Staphylococci count (CPS) and MRSA prevalence. Of 486 BTM samples tested, 12 samples (2.5%) resulted positive for the presence of MRSA. Great genetic diversity was found among the isolates: ST1/t127 and t174/IVa, ST5/t688/V, ST8/t unknown/IVa/V, ST45/t015/IVa, ST71/t524/V, ST88/t786/Iva, ST398/t011 and t899/IVa/V and ST2781/t1730/V. All isolates were pvl-negative and icaA positive. The majority of strains (58%) carried the ses (sec, seh, seg, seo, sem and sen) genes. All tested strains resulted susceptible to amikacin, cephalotin, cloramphenicol, gentamycin, trimethoprim - sulfamethoxazole, tobramycin and vancomycin, and variably resistant to ampicillin, oxacillin and tetracycline. No statistical association between the CPS count and MRSA detection was found in the MRSA-positive samples. Although some of the spa-types and STs detected in our survey are known to cause human infections, raw milk from Italian herds in the considered area is not a common source of MRSA. Nonetheless, it is necessary to assess the risk of foodborne infection and the risk related to the handling of milk. PMID:27217357

  9. Herd-prevalence of Coxiella burnetii (Q fever) antibodies in dairy cattle farms based on bulk tank milk analysis

    Institute of Scientific and Technical Information of China (English)

    Mohammad Khalili; Ehsanollah Sakhaee; Mohammad Reza Aflatoonian; Naser Shahabi-Nejad

    2011-01-01

    Objective: To determine the prevalence of Coxiella burnetii (C. burnetii) antibody positive randomly selected dairy herds in southeast Iran (Kerman). Methods: Bulk tank milk samples were collected randomly from 44 sufficiently large commercial dairy herds, included near 12 000 dairy cattle, in Kerman (The largest province of Iran), southeast Iran. The samples were tested for antibodies against C. burnetii using the commercial CHEKIT® Q fever antibody ELISA Test Kit (Idexx, Liebefeld-Bern, Switzerland). Results: The prevalence of positive, negative and intermediate herds were 45.4%, 43.2% and 11.4%, respectively. Conclusions: The result supports the hypothesis of high prevalence and endemic pattern of Q fever in Iran. This investigation highlights the importance of further studies on Q fever in Iran.

  10. Diagnostic performance of an indirect enzyme-linked immunosorbent assay (ELISA) to detect bovine leukemia virus antibodies in bulk-tank milk samples.

    Science.gov (United States)

    Nekouei, Omid; Durocher, Jean; Keefe, Greg

    2016-07-01

    This study assessed the diagnostic performance of a commercial ELISA for detecting bovine leukemia virus antibodies in bulk-tank milk samples from eastern Canada. Sensitivity and specificity of the test were estimated at 97.2% and 100%, respectively. The test was recommended as a cost-efficient tool for large-scale screening programs.

  11. Quality of bulk tank milk samples from Danish dairy herds based on real-time polymerase chain reaction identification of mastitis pathogens

    DEFF Research Database (Denmark)

    Katholm, Jørgen; Bennedsgaard, T.W.; Koskinen, M.T.;

    2012-01-01

    Results of a commercial real-time PCR analysis for 11 mastitis pathogens from bulk tank milk (BTM) samples from all 4,258 Danish dairy herds in November 2009 to January 2010 were compared with somatic cell count (SCC) and total bacteria count (TBC) estimates in BTM. For Streptococcus agalactiae, ...

  12. Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland

    Directory of Open Access Journals (Sweden)

    Stephan Roger

    2002-06-01

    Full Text Available Abstract Background Since Mycobacterium avium subspecies paratuberculosis (MAP was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific IS900 insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of Enterobacteriaceae. Results 273 (19.7% of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p >> 0.05 differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. Enterobacteriaceae occur as often in IS900 PCR-positive as in PCR-negative milk samples. Conclusion This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.

  13. Relationship among specific bacterial counts and total bacterial and somatic cell counts and factors influencing their variation in ovine bulk tank milk.

    Science.gov (United States)

    de Garnica, M L; Linage, B; Carriedo, J A; De La Fuente, L F; García-Jimeno, M C; Santos, J A; Gonzalo, C

    2013-02-01

    To analyze the relationship among the counts of different organisms and total bacterial count (BTTBC) and somatic cell count (BTSCC) as determined in dairy laboratories in ovine bulk tank milk, 751 bulk tank milk samples from 205 dairy sheep flocks belonging to Consortium for Ovine Promotion (CPO) were collected between January and December 2011. Four samplings were carried out in each flock, once per season, throughout 1 yr. Variables analyzed were bulk tank counts of thermoduric, psychrotrophic, coliform, and gram-positive catalase-negative cocci (GPCNC) bacterial groups. Thermoduric, psychrotrophic, and coliform species were significantly related to BTTBC, whereas GPCNC were correlated with both BTTBC and BTSCC variables. Highest counts were for psychrotroph and coliform groups, and a moderate to high correlation (r=0.51) was found between both variables, indicating that poor cleaning practices in the flocks tend to select for less-resistant organisms, such as gram-negative rods. In addition, BTTBC correlated with BTSCC (r=0.42). Some variation factors for specific bacterial counts, such as breed, season, milking type, dry therapy, and milk yield, were also analyzed. Flock information was collected from flock books, annual audits, and the CPO traceability system. Psychrotrophs and coliforms had elevated counts in winter, whereas GPCNC were higher in summer and in hand-milked flocks. Dry therapy contributed to the reduction in psychrotrophic bacteria; therefore, some strains of mammary pathogens could also be psychrotrophic bacteria. Results of this study would be helpful for troubleshooting milk quality problems and developing premium payment systems in dairy sheep.

  14. Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy. An epidemiological study

    Directory of Open Access Journals (Sweden)

    Roberto Rosmini

    2010-01-01

    Full Text Available The goal of the study was to genotypically compare S. aureus isolates from mastitis milk and raw milk to identify therelation between strains and to assess the enterotoxigenicity of the isolates. Eighty-three Staphylococcus aureus isolatesrecovered from cows and bulk tank milk of five farms in northern Italy were compared genotypically. The genes for theenterotoxins A, D, G and I, but not for B, C, E and H and the toxic shock syndrome toxin 1 (TSST-1, were detected byPCR amplification. Macrorestriction analysis with the restrictions enzyme SmaI revealed 14 pulsed-field gel electrophoresispatterns. These were in part different from each other only in a few fragments and thus displayed a closeclonal relation. The results of the present investigation showed that identical or closely related clones seemed to beresponsible for the cases of bovine mastitis in the farms investigated and partly responsible for contamination of bulktank milk.

  15. Management, nutrition, and lactation performance are related to bulk tank milk de novo fatty acid concentration on northeastern US dairy farms.

    Science.gov (United States)

    Woolpert, M E; Dann, H M; Cotanch, K W; Melilli, C; Chase, L E; Grant, R J; Barbano, D M

    2016-10-01

    This study investigated the relationship of management practices, dietary characteristics, milk composition, and lactation performance with de novo fatty acid (FA) concentration in bulk tank milk from commercial dairy farms with Holstein, Jersey, and mixed-breed cows. It was hypothesized that farms with higher de novo milk FA concentrations would more commonly use management and nutrition practices known to optimize ruminal conditions that enhance de novo synthesis of milk FA. Farms (n=44) located in Vermont and northeastern New York were selected based on a history of high de novo (HDN; 26.18±0.94g/100g of FA; mean ± standard deviation) or low de novo (LDN; 24.19±1.22g/100g of FA) FA in bulk tank milk. Management practices were assessed during one visit to each farm in March or April, 2014. Total mixed ration samples were collected and analyzed for chemical composition using near infrared spectroscopy. We found no differences in days in milk at the farm level. Yield of milk fat, true protein, and de novo FA per cow per day were higher for HDN versus LDN farms. The HDN farms had lower freestall stocking density (cows/stall) than LDN farms. Additionally, tiestall feeding frequency was higher for HDN than LDN farms. No differences between HDN and LDN farms were detected for dietary dry matter, crude protein, neutral detergent fiber, starch, or percentage of forage in the diet. However, dietary ether extract was lower for HDN than LDN farms. This research indicates that overcrowded freestalls, reduced feeding frequency, and greater dietary ether extract content are associated with lower de novo FA synthesis and reduced milk fat and true protein yields on commercial dairy farms. PMID:27522424

  16. Short communication: Methicillin-resistant Staphylococcus aureus in bulk tank milk of dairy cows and effect of swine population density.

    Science.gov (United States)

    Locatelli, C; Cremonesi, P; Bertocchi, L; Zanoni, M G; Barberio, A; Drigo, I; Varisco, G; Castiglioni, B; Bronzo, V; Moroni, P

    2016-03-01

    The methicillin-resistant Staphylococcus aureus (MRSA) has recently frequently been reported in dairy cattle, usually with low prevalence. The livestock-associated MRSA (LA-MRSA) ST398 is especially involved in cases of subclinical and clinical mastitis. Swine carry LA-MRSA without clinical symptoms and are considered its reservoir and shedder. People exposed to swine are particularly at risk of LA-MRSA colonization. Environments with relevant livestock density are a demonstrated risk factor for humans to be carriers of a LA-MRSA. This work investigated dairy farms located in an area with a high livestock density, mainly represented by swine. Bulk tank milk samples from 224 dairy farms were collected, and their status was defined as MRSA-positive or MRSA-negative based on culture on chromogenic medium. The number of fattening swine and of fattening swine herds was calculated in an area of 3 km around each dairy farm through georeferencing. The probability of a Staphylococcus aureus-positive dairy farm to be MRSA positive based on the extent of potential infective pressure due to swine density was calculated. Both the number of swine herds and the number of swine were associated with the MRSA status of dairy herds. The 9 MRSA isolated were typed by multi-locus sequence typing and spa-typing, and characterized for their virulence factors and antimicrobial resistance profiles. The ST and spa-types detected are consistent with those present in the Italian swine population. Virulence and resistance profiles are mostly consistent with the types detected. This work provides the first evidence of the epidemiological challenge exerted by the density of the swine population on MRSA in dairy cows. PMID:26805972

  17. Associations of risk factors with somatic cell count in bulk tank milk on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Gamroth, M; Richert, R; Ruegg, P L; Stiglbauer, K E; Schukken, Y H

    2013-06-01

    In the past decade, the demand for organic agricultural products has increased rapidly in the United States and worldwide. Milk quality research is of major interest to both consumers and dairy farmers alike. However, scientific data on milk quality, herd management methods, and animal welfare on organic farms in the United States has been lacking before the research from this study. The objective of this study was to evaluate the association of bulk tank milk somatic cell count (SCC) with management characteristics on organic and conventional dairy farms in New York, Oregon, and Wisconsin. Data from similarly sized organic farms (n=192), conventional nongrazing farms (n=64), and conventional grazing farms (n=36) were collected at a single farm visit. Of the 292 farms visited, 290 bulk tank milk samples were collected. Overall, no difference in SCC was observed between the conventional and organic grazing systems. Two models were created to assess the effects of various management and herd characteristics on the logarithmic transformation of the SCC (LSCC), one using data from all herds and one using data from organic herds only. From the total herd model, more grain fed per cow per day was negatively associated with LSCC, whereas a positive bulk tank culture for Staphylococcus aureus and years that a farmer reported being in the dairy business were both positively associated with LSCC. In the organic herd model, a seasonal effect indicated that LSCC tended to increase in the summer and decrease in the winter. Grain fed per cow per day, the use of anionic salts in transition-cow diets, the use of gloves during milking, and regular use of a quarantine unit at milking were all negatively associated with LSCC. Similar to the total herd model, a Staph. aureus-positive bulk tank culture was positively associated with LSCC in the organic model. Standard plate count was also positively associated with LSCC in the organic model. Several variables that were associated with

  18. Prevalence of contagious and environmental mastitis-causing bacteria in bulk tank milk and its relationships with milking practices of dairy cattle herds in São Miguel Island (Azores).

    Science.gov (United States)

    Azevedo, Carla; Pacheco, Diana; Soares, Luísa; Romão, Ricardo; Moitoso, Mónica; Maldonado, Jaime; Guix, Roger; Simões, João

    2016-02-01

    This study aimed to assess the degree of contamination of bulk tank milk (BTM) by Staphylococcus spp. and coliform bacteria and to identify major milking practices that help perpetuate them in dairy cattle herds in São Miguel Island. In July 2014, BTM was sampled and a survey concerning local milking practices was conducted on 100 herds. Semi quantitative multiplex polymerase chain reaction detected coagulase-negative staphylococci, Escherichia coli, Staphylococcus aureus, and other coliform bacteria (Klebsiella oxytoca, Klebsiella pneumoniae, and Serratia marcescens) in 100, 75, 59, and 35 % of BTM, respectively. According to multivariable univariate models, on herds not using hot water for cleaning the milking machine and teat liners, there was at least 3.4 more odds (P < 0.01) to have S. aureus or coliform bacteria contamination in BTM. The likelihood of finding S. aureus in BTM was higher (P < 0.001) on herds without high hygiene during milking, when milking mastitic cows at the end, on abrupt cessation of milking at dry-off, and official milk control implementation. The glove use also favored (odds ratio (OR) 5.8; P < 0.01) the detection of coliform bacteria in BTM. Poor milking practices identified in this study should be avoided in order to decrease S. aureus and coliform bacteria contamination of BTM. Other factors associated with milk quality in São Miguel Island also should be further investigated.

  19. Associations among milk quality indicators in raw bulk milk.

    Science.gov (United States)

    Pantoja, J C F; Reinemann, D J; Ruegg, P L

    2009-10-01

    The objective of this study was to determine characteristics and associations among bulk milk quality indicators from a cohort of dairies that used modern milk harvest, storage, and shipment systems and participated in an intensive program of milk quality monitoring. Bulk milk somatic cell count (SCC), total bacteria count (TBC), coliform count (CC), and laboratory pasteurization count (LPC) were monitored between July 2006 and July 2007. Bulk milk samples were collected 3 times daily (n = 3 farms), twice daily (n = 6 farms), once daily (n = 4 farms), or once every other day (n = 3 farms). Most farms (n = 11) had direct loading of milk into tankers on trucks, but 5 farms had stationary bulk tanks. The average herd size was 924 cows (range = 200 to 2,700), and daily milk produced per herd was 35,220 kg (range = 7,500 to 105,000 kg). Thresholds for increased bacterial counts were defined according to the 75th percentile and were >8,000 cfu/mL for TBC, >160 cfu/mL for CC, and >or=310 cfu/mL for LPC. Means values were 12,500 (n = 7,241 measurements), 242 (n = 7,275 measurements), and 226 cfu/mL (n = 7,220 measurements) for TBC, CC, and LPC, respectively. Increased TBC was 6.3 times more likely for bulk milk loads with increased CC compared with loads containing fewer coliforms. Increased TBC was 1.3 times more likely for bulk milk with increased LPC. The odds of increased TBC increased by 2.4% for every 10,000-cells/mL increase in SCC in the same milk load. The odds of increased CC increased by 4.3% for every 10,000-cells/mL increase in SCC. The odds of increased CC increased by 1% for every 0.1 degrees C increase in the milk temperature upon arrival at the dairy plant (or at pickup for farms with bulk tank). Laboratory pasteurization count was poorly associated with other milk quality indicators. Seasonal effects on bacterial counts and milk temperature varied substantially among farms. Results of this study can be used to aid the interpretation and analysis of

  20. Bulk tank milk surveillance as a measure to detect Coxiella burnetii shedding dairy goat herds in the Netherlands between 2009 and 2014.

    Science.gov (United States)

    Van den Brom, R; Santman-Berends, I; Luttikholt, S; Moll, L; Van Engelen, E; Vellema, P

    2015-06-01

    In the period from 2005 to 2009, Coxiella burnetii was a cause of abortion waves at 28 dairy goat farms and 2 dairy sheep farms in the Netherlands. Two years after the first abortion waves, a large human Q fever outbreak started mainly in the same region, and aborting small ruminants were regarded as most probable source. To distinguish between infected and noninfected herds, a surveillance program started in October 2009, based on PCR testing of bulk tank milk (BTM) samples, which had never been described before. The aim of this study was to analyze the effectiveness of this surveillance program and to evaluate both the effect of culling of pregnant dairy goats on positive farms and of vaccination on BTM results. Bulk tank milk samples were tested for C. burnetii DNA using a real-time PCR, and results were analyzed in relation to vaccination, culling, and notifiable (officially reported to government) C. burnetii abortion records. In spring and autumn, BTM samples were also tested for antibodies using an ELISA, and results were evaluated in relation to the compulsory vaccination campaign. Between October 2009 and April 2014, 1,660 (5.6%) out of 29,875 BTM samples from 401 dairy goat farms tested positive for C. burnetii DNA. The percentage of positive samples dropped from 20.5% in 2009 to 0.3% in 2014. In a multivariable model, significantly higher odds of being PCR positive in the BTM surveillance program were found in farms of which all pregnant dairy goats were culled. Additionally, the risk for C. burnetii BTM PCR positivity significantly decreased after multiple vaccinations. Bulk tank milk ELISA results were significantly higher after vaccination than before. The ELISA results were higher after multiple vaccinations compared with a single vaccination, and ELISA results on officially declared infected farms were significantly higher compared with noninfected farms. In conclusion, BTM surveillance is an effective and useful tool to detect C. burnetii shedding

  1. Bulk tank milk ELISA for detection of antibodies to Mycobacterium avium subsp paratuberculosis: Correlation between repeated tests and within-herd antibody-prevalence

    DEFF Research Database (Denmark)

    Nielsen, Søren Saxmose; Toft, Nils

    2014-01-01

    Detection of bulk tank milk (BTM) antibodies using ELISA (BTM-ELISA) may constitute an inexpensive test for surveillance of Mycobacterium avium subsp. paratuberculosis (MAP) infection in dairy cattle herds provided that the test is accurate and consistent. The objectives of this study were...... Danish Holstein herds over a period of one year. All samples were tested using a commercial indirect ELISA for detection of MAP specific antibodies. The individual cow's results were dichotomised and used to estimate the within-herd antibody prevalence at each test-date. These prevalences were...... then combined with the ELISA reading on the BTM test-date closest to the cow-level test-date. A mixed-effect analysis of covariance with autoregressive type 1 correlation structure was carried out using the log-transformed BTM-ELISA results as outcome. This model was used to assess the correlation between...

  2. Modeling of Mycobacterium avium subsp. paratuberculosis in farm bulk tank milk

    DEFF Research Database (Denmark)

    Okura, Hisako; Nielsen, Søren Saxmose; Toft, Nils

    2012-01-01

    super-shedders. At the prevalence of 15%, discarding milk from test positive cows would result in discarding 11% of milk and reduce the MAP level by 80%. The model was relatively simple yet capable of capturing true infection status and associated contributions from milk and feces. Further knowledge on...... simulating the effect of different control options at different infection prevalences. The concentration of MAP in milk was estimated using a hierarchical simulation model representing individual cows in a herd while taking both direct and indirect contamination with MAP into account. Parameters included...... true within-herd infection prevalences with cows in different infection stages, which were used to estimate prevalences of milk and fecal shedders, and subsequently MAP concentrations in milk and feces. Cows in different infection stages were considered with different risks of excreting MAP testing...

  3. Prevalence and characteristics of methicillin-resistant coagulase-negative staphylococci from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons

    Directory of Open Access Journals (Sweden)

    Zweifel Claudio

    2011-01-01

    Full Text Available Abstract Background Methicillin-resistant coagulase-negative staphylococci (MR-CNS are of increasing importance to animal and public health. In veterinary medicine and along the meat and milk production line, only limited data were so far available on MR-CNS characteristics. The aim of the present study was to evaluate the prevalence of MR-CNS, to identify the detected staphylococci to species level, and to assess the antibiotic resistance profiles of isolated MR-CNS strains. Results After two-step enrichment and growth on chromogenic agar, MR-CNS were detected in 48.2% of samples from livestock and chicken carcasses, 46.4% of samples from bulk tank milk and minced meat, and 49.3% of human samples. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS, 414 selected MR-CNS strains belonged to seven different species (S. sciuri, 32.6%; S. fleurettii, 25.1%; S. haemolyticus, 17.4%; S. epidermidis, 14.5%, S. lentus, 9.2%; S. warneri, 0.7%; S. cohnii, 0.5%. S. sciuri and S. fleurettii thereby predominated in livestock, BTM and minced meat samples, whereas S. epidermidis and S. haemolyticus predominated in human samples. In addition to beta-lactam resistance, 33-49% of all 414 strains were resistant to certain non-beta-lactam antibiotics (ciproflaxacin, clindamycin, erythromycin, tetracycline. Conclusions A high prevalence of MR-CNS was found in livestock production. This is of concern in view of potential spread of mecA to S. aureus (MRSA. Multiresistant CNS strains might become an emerging problem for veterinary medicine. For species identification of MR-CNS isolated from different origins, MALDI-TOF MS proved to be a fast and reliable tool and is suitable for screening of large sample amounts.

  4. Risk analysis of investments in-farm milk cooling tanks

    Directory of Open Access Journals (Sweden)

    Danielle D. Sant´Anna

    2003-06-01

    Full Text Available A risk analysis for the installation of milk cooling tanks (250, 500 and 1,000 L on Brazilian rural properties was conducted in this study. The results showed that all investments had a return higher than the annual 12% minimum rate of attractiveness. There was a direct relationship between tank size and investment profitability and an inverse relation between size and risk. The probability of achieving returns lower than the opportunity cost was highest for the smallest tank (42%. In order to make the investment in small cooling tanks more attractive, the dairy industry incentives offered to farmers for supplying cooled milk could be increased. However, this approach might make investments in bulk milk collection by dairy companies infeasible. Thus, a recommendable strategy for a successful modernization of the Brazilian dairy sector’s inbound logistics would be to promote an increase in the volume of the milk produced per farm.

  5. A retrospective evaluation of a Bovine Herpesvirus-1 (BHV-1) antibody ELISA on bulk-tank milk samples for classification of the BHV-1 status of Danish dairy herds

    DEFF Research Database (Denmark)

    Nylin, Britta; Strøger, Ulla; Rønsholt, Leif

    2000-01-01

    Bulk-tank milk samples analysed in a Bovine Herpesvirus-1 (BHV-1) blocking ELISA are still in use in the Danish BHV-1 programme as a tool to classify dairy herds as BHV-1 infected or BHV-1 free herds. in this retrospective study, we used data from the Danish BHV-1 eradication campaign to evaluate...... performance characteristics of the BHV-1 blocking ELISA in 1039 BHV-1-seropositive and 502 repeatedly BHV-1-negative dairy herds using the results of blood testing of the individual animals as the true infection status. At a cut-off value of 30% blocking reaction, the herd-level relative sensitivity...

  6. The Application of Bulk Tank Somatic Cell Counts to Monitoring Mastitis Levels in Dairy Herds

    OpenAIRE

    Meek, A H; Barnum, D. A.

    1982-01-01

    The objective of this study was to investigate the feasibility of developing a system whereby measurements taken on bulk tank milk samples could be used to monitor the level of subclinical mastitis in dairy herds.

  7. Isolation of Prototheca spp. from cows with mastitis, bulk tanks milk and in the environment of the animals/ Isolamento de Prototheca spp. de vacas com mastite, de leite de tanques de expansão e do ambiente dos animais

    Directory of Open Access Journals (Sweden)

    Agda de Godoy

    2007-07-01

    Full Text Available The dairy cattle mammary gland infections cause serious economic losses to dairy farmers due to the decrease in milk production, therapeutic procedures and culling of chronic infected animals. High incidence of mastitis in herds also alters both the composition and the quality of the milk. Mastitis pathogens can also cause infections and poisoning in humans. In the last years, emphasis has been given to intramammary infections caused by the genus Prototheca which, besides their zoonotic characteristics, are considered mastitis pathogens of persistent infection and are refractory to traditional therapeutic procedures. The objective of this work was the isolation and identification of Prototheca spp. from milk samples collected from bulk tanks and milk cans, cows presenting mastitis and the dairy herd environment. Milk samples were collected from 81 bulk tanks and milk cans of 81 dairy herds. Prototheca zopfii was identified in milk samples in 10 dairy herds. From these, eight dairy herds were studied regarding Prototheca spp. mastitis and environmental occurrence as well as the main mastitis bacterial agents. Bacteria, algae and yeasts were isolated from 324 milk samples from 197 cows. P. zopfii was isolated in three dairy herds from eleven milk samples from five cows with clinical and subclinical mastitis. In these dairy herds with positive isolation of P. zopfii the agent was isolated from the herd environment, excrements of the calves and teat cup rubbers. The results of this work demonstrate the importance of isolation in bulk tanks as an indicative of Prototheca spp. presence in dairy herds.As infecções da glândula mamária de vacas leiteiras acarretam sérios prejuízos ao produtor pela diminuição da produção leiteira, tratamento e descarte de animais com infecções crônicas. Elevada incidência de mastite no rebanho também altera a composição e qualidade do leite. Agentes de mastite podem causar infecções ou intoxicações no

  8. 49 CFR 172.331 - Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. 172.331 Section 172.331 Transportation Other Regulations... packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. (a) Each...

  9. Socioeconomic and technical assistance factors related to total bacteria count and somatic cell count of milk from bulk tanks in southern Minas Gerais State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcel Gomes Paixão

    2015-07-01

    Full Text Available The purpose of this survey was to evaluate the socioeconomic and technical assistance profiles of dairy farmers from six districts in the south of Minas Gerais state, Brazil, and to identify the possible risk factors associated with total milk bacteria count (TBC above 43,000 CFU mL-1 and bulk milk somatic cell count (BMSCC above 595,000 cells mL-1. Most of the producers were between 41 and 60 years of age (48.9%, 74.2% did not reach high school, and 72.3% of the respondents were satisfied with their profession, although 63% would not recommend dairy farming to their children. Only 34.7% used periodic technical assistance, but 59.1% consulted it in cases of doubt. The risk factors found in the final multivariable regression models were: TBC (Did not consult technical assistance in case of doubt, OR 3.97, P=0.030; Retirement, OR 9.32, P=0.041 and BMSCC (Producer does not reside on farm, OR 4.06, P=0.046; Presence of technical assistance OR 3.29, P=0.041. It can be concluded that the search for emergency technical assistance, as reported by farmers, was effective against the TBC problems; however, it was ineffective for controlling mastitis in the herd and reducing BMSCC levels. The 10 step mastitis control program from the National Mastitis Council needs to be included on the surveyed farms, especially the permanent advisory technical assistance from veterinarians, aiming towards the establishment of goals for udder health status, reviews and records.

  10. QUALIDADE DE LEITE CRU PRODUZIDO E ARMAZENADO EM TANQUES DE EXPANSÃO NO ESTADO DE GOIÁS RAW QUALITY OF MILK PRODUCED AND STORED IN BULK TANKS IN GOIAS STATE, BRAZIL

    Directory of Open Access Journals (Sweden)

    Marcele Louise Tadaieski Arruda

    2008-12-01

    raw milk quality in samples obtained from bulk tanks in the Goiás State. Thirty raw milk  samples of 1000 mL were harvest. It was performed the following analyses: total bacterial count (TBC by Flow Citometry, search for the presence of residues of antimicrobial agents by the Delvotest® SP kit and investigation for the fraud by adding to milk the following substances: hydrogen peroxide, formaldehyde, sodium bicarbonate and sodium hydroxide. It was not found any residues of antimicrobial agents in all (100% samples. Physicochemical test also showed the absence of hydrogen peroxide, formaldehyde, sodium bicarbonate and sodium hydroxide in milk. About TBC, it was observed that 30% (9/30 of bulk tanks showed TBC>106 CFU/mL, in 46.67% (14/30 the TBC was between 105 to 106 CFU/mL and 23.33% (7/30 showed TBC <105 CFU/mL. Means observed were TBC of 9.2 X 106, TBC of 4.5 X 105, TBC of 5.1 X 104, respectively. From bulk tanks with TBC>106 CFU/mL, 100% were available for common utilization and had significantly higher values for TBC (P<0.05. Educational standards and training about the hygienic procedures applied to milk obtainment and to milking equipments, a well implemented mastitis control program and correct use of milk cooling system after milking must be adopted in order to provide a better raw milk quality. These measures provide benefits to industry due to the higher income of quality raw material. It also provides benefits for the farmer, since they can receive a better wage from dairy companies that pay higher for better quality products; to the trade because quality products have a longer shelf-life and to consumer, that can have an increase in quality of life.

    KEY WORDS: CFU, raw milk,  quality, residues.

  11. Implications of PCR and ELISA results on the routes of bulk-tank contamination with Mycobacterium avium ssp. paratuberculosis.

    Science.gov (United States)

    Beaver, A; Cazer, C L; Ruegg, P L; Gröhn, Y T; Schukken, Y H

    2016-02-01

    Mycobacterium avium ssp. paratuberculosis (MAP), the etiologic agent of Johne's disease in dairy cattle, may enter the bulk tank via environmental contamination or direct excretion into milk. Traditionally, diagnostics to identify MAP in milk target either MAP antibodies (by ELISA) or the organism itself (by culture or PCR). High ELISA titers may be directly associated with excretion of MAP into milk but only indirectly linked to environmental contamination of the bulk tank. Patterns of bulk-milk ELISA and bulk-milk PCR results could therefore provide insight into the routes of contamination and level of infection or environmental burden. Coupled with questionnaire responses pertaining to management, the results of these diagnostic tests could reveal correlations with herd characteristics or on-farm practices that distinguish herds with high and low environmental bulk-tank MAP contamination. A questionnaire on hygiene, management, and Johne's specific parameters was administered to 292 dairy farms in New York, Oregon, and Wisconsin. Bulk-tank samples were collected from each farm for evaluation by real-time PCR and ELISA. Before DNA extraction and testing of the unknown samples, bulk-milk template preparation was optimized with respect to parameters such as MAP fractionation patterns and lysis. Two regression models were developed to explore the relationships among bulk-tank PCR, ELISA, environmental predictors, and herd characteristics. First, ELISA optical density (OD) was designated as the outcome in a linear regression model. Second, the log odds of being PCR positive in the bulk tank were modeled using binary logistic regression with penalized maximum likelihood. The proportion of PCR-positive bulk tanks was highest for New York and for organic farms, providing a clue as to the geographical patterns of MAP-positive bulk-tank samples and relationship to production type. Bulk-milk PCR positivity was also higher for large relative to small herds. The models

  12. Avaliação da sensibilidade da cultura de leite do tanque para isolamento de agentes contagiosos da mastite bovina Evaluation of the sensitivity of bulk tank milk cultures for the isolation of contagious bovine mastitis pathogens

    Directory of Open Access Journals (Sweden)

    Maria Aparecida V. P. Brito

    1998-01-01

    ,7% para os quartos mamários. S. aureus foi isolado de todas três amostras do tanque dos rebanhos A, B e D. Somente a terceira amostra do rebanho C foi positiva para S. aureus. S agalactiae foi recuperado de todas as amostras do rebanho D, duas do rebanho C e de uma do rebanho A. Todas as amostras do tanque dos rebanhos A, B, C e D apresentaram contaminação com coliformes e somente uma das amostras coletadas na plataforma de recepção da indústria foi negativa para coliformes. Leveduras foram isoladas de 16 amostras coletadas na indústria e de todas amostras do tanque dos rebanhos A, B, C e D. Não foram isolados coliformes ou leveduras dos quartos mamários dos animais destes rebanhos, sugerindo que ocorreu contaminação do leite durante ou após a ordenha, provavelmente devido a deficiências nos processos de limpeza e higienização. A análise dos resultados das culturas do leite do tanque mostrou que o exame foi específico para detectar os patógenos contagiosos da mastite. A sensibilidade do teste aumentou quando se examinaram mais de duas amostras consecutivas.Samples of bulk tank milk from 33 herds were collected at the dairy processing plant and cultured, as a means of detecting specific (contagious bovine mastitis pathogens. Somatic cell counts (SCC were made on a Fossomatic 90. Two and three weekly consecutive samples were obtained from 13 and 12 herds, respectively. Only one sample was examined from eight herds. Three daily consecutive samples of bulk milk and individual quarter samples from all lactating cows from four herds (A, B, C and D were also examined. Milk from individual quarters were cultured on blood agar, while tank milk samples were cultured on TKT, Mannitol Salt, MacConkey agars and Sabouraud containing chloramphenicol. Staphylococcus aureus was recovered from 26 of the 33 herds sampled in the dairy processing plant. Nine of these samples also contained Streptococcus agalactiae. Nine herds had SCC above 500,000 ml-1. The remaining 23

  13. Temporal trends in bulk tank somatic cell count and total bacterial count in Irish dairy herds during the past decade.

    Science.gov (United States)

    Berry, D P; O'Brien, B; O'Callaghan, E J; Sullivan, K O; Meaney, W J

    2006-10-01

    The objective of this study was to document temporal trends in bulk tank somatic cell count (SCC) and total bacterial counts (TBC) in Irish dairy herds during the years 1994 to 2004. Three milk processors participated in the study, providing data on 2,754,270 individual bulk tank SCC and 2,056,992 individual bulk tank TBC records from 9,113 herds. Somatic cell counts decreased during the years 1994 to 2000, followed by an annual increase thereafter of more than 2,000 cells/mL. A tendency existed for TBC to decrease over time. Across all years, bulk tank SCC were the lowest in April and highest in November; TBC were the lowest in May and highest in December. The significant seasonal pattern observed in herd SCC and TBC was an artifact of seasonal calving in Ireland. In general, herds selling more milk had lower bulk tank SCC and TBC. Herds having the highest SCC (i.e., > 450,000 cells/mL) and the lowest SCC (i.e., < or = 150,000 cells/mL) both contributed substantially to the mean SCC of the milk pool collected by the milk processors. Derived transition matrices showed that between adjacent years, herds had the greatest probability of remaining in the same annual mean SCC or TBC category.

  14. Risk factors associated with bulk tank standard plate count, bulk tank coliform count, and the presence of Staphylococcus aureus on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Gamroth, M; Richert, R; Ruegg, P L; Stiglbauer, K E; Schukken, Y H

    2013-01-01

    The purpose of this study was to assess the association of bulk tank milk standard plate counts, bulk tank coliform counts (CC), and the presence of Staphylococcus aureus in bulk tank milk with various management and farm characteristics on organic and conventional dairy farms throughout New York, Wisconsin, and Oregon. Data from size-matched organic farms (n=192), conventional nongrazing farms (n=64), and conventional grazing farms (n=36) were collected at a single visit for each farm. Of the 292 farms visited, 290 bulk tank milk samples were collected. Statistical models were created using data from all herds in the study, as well as exclusively for the organic subset of herds. Because of incomplete data, 267 of 290 herds were analyzed for total herd modeling, and 173 of 190 organic herds were analyzed for the organic herd modeling. Overall, more bulk tanks from organic farms had Staph. aureus cultured from them (62% of organic herds, 42% conventional nongrazing herds, and 43% of conventional grazing herds), whereas fewer organic herds had a high CC, defined as ≥50 cfu/mL, than conventional farms in the study. A high standard plate count (×1,000 cfu/mL) was associated with decreased body condition score of adult cows and decreased milk production in both models. Several variables were significant only in the model created using all herds or only in organic herds. The presence of Staph. aureus in the bulk tank milk was associated with fewer people treating mastitis, increased age of housing, and a higher percentage of cows with 3 or fewer teats in both the organic and total herd models. The Staph. aureus total herd model also showed a relationship with fewer first-lactation animals, higher hock scores, and less use of automatic takeoffs at milking. High bulk tank CC was related to feeding a total mixed ration and using natural service in nonlactating heifers in both models. Overall, attentive management and use of outside resources were useful with regard to CC

  15. Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count

    NARCIS (Netherlands)

    Koop, G.; Dik, N.; Nielen, M.; Lipman, L.J.A.

    2010-01-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms,

  16. Investigations of waste heat recovery from bulk milk cooler

    Directory of Open Access Journals (Sweden)

    S.N. Sapali

    2014-11-01

    Full Text Available Bulk milk coolers are used to chill the milk from its harvest temperature of 35–4 °C to arrest the bacterial growth and maintain the quality of harvested milk. Milk chilling practices are energy intensive with low coefficient of performance (COP of about 3.0. Increased energy cost concern encouraged an investigation of heat recovery from bulk milk cooler as one conservation alternative for reducing water heating cost in dairy industry. Heat dissipated to atmosphere through condenser is recovered to improve the energy efficiency of plant. The waste heat is utilized to heat the water which is used to clean the milk processing equipments thus saving thermal or electrical energy used to heat the water separately. Shell and coil type heat exchanger is designed and used to recover the waste heat during condensation process. Heat rejected in condensation process consists of superheat and latent heat of the refrigerant. In this work, attempt has been made to recover complete superheat along with part of latent heat which is a present research issue. The results show that complete superheat and 35% of latent heat is recovered. Heat recovery rate is measured for various mass flow rates. Water is flowing on shell side and refrigerant through tubes. The effectiveness of the heat exchanger is determined and the results achieved are presented in this paper. Significant improvements have been achieved and COP of the system is increased from 3 to 4.8.

  17. Efficiency of cleaning procedure of milking equipment and bacterial quality of milk

    OpenAIRE

    Anna Sandrucci; Lucio Zanini; Milena Brasca; Maddalena Zucali; Luciana Bava

    2010-01-01

    The cleaning and sanitation of milking equipment could be consider a critical point in the milking procedure because a cleaning failure could influence the level of bacteria contamination of bulk tank milk. Aim of the study is to monitor the cleaning procedures of milking systems in 7 dairy cows farms in Lombardy and to find a relation between efficacy of cleaning system and the bacterial quality of bulk tank milk, remaining washing water through milking equipment and teat cup surface. Cleani...

  18. Milk adulteration: Detection of bovine milk in bulk goat milk produced by smallholders in northeastern Brazil by a duplex PCR assay.

    Science.gov (United States)

    Rodrigues, N P A; Givisiez, P E N; Queiroga, R C R E; Azevedo, P S; Gebreyes, W A; Oliveira, C J B

    2012-05-01

    The aim of this study was to investigate the adulteration of goat milk produced by smallholders in semiarid northeastern Brazil with bovine milk as an adulterant. The study was requested by the association of smallholder producers in the region to investigate and to inhibit adulteration practices as a need to ensure the quality and safety of goat milk. A duplex PCR assay has been developed and standardized. Further validation was performed in 160 fresh bulk goat milk samples. The detection limit of the duplex PCR was 0.5% bovine milk in goat milk and the results indicated that 41.2% of the goat milk presented to market was positive for bovine milk. Making the test available to the association of producers, together with extension activities, have been applied to reduce adulteration in goat milk sold to small-scale dairy plants and to ensure the species origin for goat milk in the state of Paraíba.

  19. Optimizing bulk milk dioxin monitoring based on costs and effectiveness.

    Science.gov (United States)

    Lascano-Alcoser, V H; Velthuis, A G J; van der Fels-Klerx, H J; Hoogenboom, L A P; Oude Lansink, A G J M

    2013-07-01

    Dioxins are environmental pollutants, potentially present in milk products, which have negative consequences for human health and for the firms and farms involved in the dairy chain. Dioxin monitoring in feed and food has been implemented to detect their presence and estimate their levels in food chains. However, the costs and effectiveness of such programs have not been evaluated. In this study, the costs and effectiveness of bulk milk dioxin monitoring in milk trucks were estimated to optimize the sampling and pooling monitoring strategies aimed at detecting at least 1 contaminated dairy farm out of 20,000 at a target dioxin concentration level. Incidents of different proportions, in terms of the number of contaminated farms, and concentrations were simulated. A combined testing strategy, consisting of screening and confirmatory methods, was assumed as well as testing of pooled samples. Two optimization models were built using linear programming. The first model aimed to minimize monitoring costs subject to a minimum required effectiveness of finding an incident, whereas the second model aimed to maximize the effectiveness for a given monitoring budget. Our results show that a high level of effectiveness is possible, but at high costs. Given specific assumptions, monitoring with 95% effectiveness to detect an incident of 1 contaminated farm at a dioxin concentration of 2 pg of toxic equivalents/g of fat [European Commission's (EC) action level] costs €2.6 million per month. At the same level of effectiveness, a 73% cost reduction is possible when aiming to detect an incident where 2 farms are contaminated at a dioxin concentration of 3 pg of toxic equivalents/g of fat (EC maximum level). With a fixed budget of €40,000 per month, the probability of detecting an incident with a single contaminated farm at a dioxin concentration equal to the EC action level is 4.4%. This probability almost doubled (8.0%) when aiming to detect the same incident but with a dioxin

  20. Optimizing bulk milk dioxin monitoring based on costs and effectiveness.

    Science.gov (United States)

    Lascano-Alcoser, V H; Velthuis, A G J; van der Fels-Klerx, H J; Hoogenboom, L A P; Oude Lansink, A G J M

    2013-07-01

    Dioxins are environmental pollutants, potentially present in milk products, which have negative consequences for human health and for the firms and farms involved in the dairy chain. Dioxin monitoring in feed and food has been implemented to detect their presence and estimate their levels in food chains. However, the costs and effectiveness of such programs have not been evaluated. In this study, the costs and effectiveness of bulk milk dioxin monitoring in milk trucks were estimated to optimize the sampling and pooling monitoring strategies aimed at detecting at least 1 contaminated dairy farm out of 20,000 at a target dioxin concentration level. Incidents of different proportions, in terms of the number of contaminated farms, and concentrations were simulated. A combined testing strategy, consisting of screening and confirmatory methods, was assumed as well as testing of pooled samples. Two optimization models were built using linear programming. The first model aimed to minimize monitoring costs subject to a minimum required effectiveness of finding an incident, whereas the second model aimed to maximize the effectiveness for a given monitoring budget. Our results show that a high level of effectiveness is possible, but at high costs. Given specific assumptions, monitoring with 95% effectiveness to detect an incident of 1 contaminated farm at a dioxin concentration of 2 pg of toxic equivalents/g of fat [European Commission's (EC) action level] costs €2.6 million per month. At the same level of effectiveness, a 73% cost reduction is possible when aiming to detect an incident where 2 farms are contaminated at a dioxin concentration of 3 pg of toxic equivalents/g of fat (EC maximum level). With a fixed budget of €40,000 per month, the probability of detecting an incident with a single contaminated farm at a dioxin concentration equal to the EC action level is 4.4%. This probability almost doubled (8.0%) when aiming to detect the same incident but with a dioxin

  1. Factors influencing variation of bulk milk antibiotic residue occurrence, somatic cell count, and total bacterial count in dairy sheep flocks.

    Science.gov (United States)

    Gonzalo, C; Carriedo, J A; García-Jimeno, M C; Pérez-Bilbao, M; de la Fuente, L F

    2010-04-01

    To study the variations of bulk tank milk variables in dairy ewe flocks and to identify the main target practices and flock groups to improve milk quality and safety, a total of 71,228 records of antibiotic residue (AR) and milk yield and 68,781 records of somatic cell count (SCC) and total bacterial count (TBC) were obtained over 5 yr from the same 209 dairy ewe flocks of the Assaf breed belonging to the Consortium for Ovine Promotion of Castilla-León (Spain). Based on a logistic regression model, year, month, semester, SCC, TBC, dry therapy, and milk yield significantly contributed to AR variation. High SCC was associated with increased AR violations. When antibiotic dry therapy was implemented, AR occurrence was higher than when this practice was not used. A polynomial monthly distribution throughout the year was observed for AR occurrence; the highest values were in autumn, coinciding with low milk yields per flock. Yearly occurrences drastically diminished from 2004 (1.36%) to 2008 (0.30%), probably as a result of effective educational programs. The mixed-model ANOVA of factors influencing variation in SCC and TBC indicated that year, month, AR, dry therapy group, milking type, and year interactions were significant variation factors for SCC and TBC; mathematical model accounted for 74.1 and 35.4% of total variance for each variable, respectively. Differences in management and hygiene practice caused significant SCC and TBC variations among flocks and within flocks throughout the 5-yr study. Over time, continuously dry treated flocks showed lower logSCC (5.80) and logTBC (4.92) than untreated (6.10 and 5.18, respectively) or discontinuously dry treated (6.01 and 5.05, respectively) flocks. Continuously dry treated flocks had lower AR occurrences than did discontinuously dry treated flocks. As a whole, AR occurrence and SCC and TBC bulk tank milk variables can be used for monitoring mammary health and milk hygiene and safety in dairy sheep throughout time.

  2. Short communication: bulk tank total bacterial count in dairy sheep: factors of variation and relationship with somatic cell count.

    Science.gov (United States)

    Gonzalo, C; Carriedo, J A; Beneitez, E; Juárez, M T; De La Fuente, L F; San Primitivo, F

    2006-02-01

    A total of 9,353 records for bulk tank total bacterial count (TBC) were obtained over 1 yr from 315 dairy ewe flocks belonging to the Sheep Improvement Consortium (CPO) in Castilla-León (Spain). Analysis of variance showed significant effects of flock, breed, month within flock, dry therapy, milking type and installation, and logSCC on logTBC. Flock and month within flock were important variation factors as they accounted for 22.0 and 22.1% of the variance, respectively. Considerable repeatability values were obtained for both random factors. Hand milking and bucket-milking machines elicited highest logTBC (5.31), whereas parlor systems with looped milkline (5.01) elicited the lowest logTBC. The implementation of dry therapy practice (5.12) showed significantly lower logTBC than when not used (5.25). Variability in logTBC among breeds ranged from 5.24 (Awassi) to 5.07 (Churra). However, clinical outbreaks of contagious agalactia did not increase TBC significantly. A statistically significant relationship was found between logTBC and logSCC, the correlation coefficient between the variables being r = 0.23. Programs for improving milk hygiene should be implemented for both total bacterial count and somatic cell count variables at the same time.

  3. Visible and near-infrared bulk optical properties of raw milk.

    Science.gov (United States)

    Aernouts, B; Van Beers, R; Watté, R; Huybrechts, T; Lammertyn, J; Saeys, W

    2015-10-01

    The implementation of optical sensor technology to monitor the milk quality on dairy farms and milk processing plants would support the early detection of altering production processes. Basic visible and near-infrared spectroscopy is already widely used to measure the composition of agricultural and food products. However, to obtain maximal performance, the design of such optical sensors should be optimized with regard to the optical properties of the samples to be measured. Therefore, the aim of this study was to determine the visible and near-infrared bulk absorption coefficient, bulk scattering coefficient, and scattering anisotropy spectra for a diverse set of raw milk samples originating from individual cow milkings, representing the milk variability present on dairy farms. Accordingly, this database of bulk optical properties can be used in future simulation studies to efficiently optimize and validate the design of an optical milk quality sensor. In a next step of the current study, the relation between the obtained bulk optical properties and milk quality properties was analyzed in detail. The bulk absorption coefficient spectra were found to mainly contain information on the water, fat, and casein content, whereas the bulk scattering coefficient spectra were found to be primarily influenced by the quantity and the size of the fat globules. Moreover, a strong positive correlation (r ≥ 0.975) was found between the fat content in raw milk and the measured bulk scattering coefficients in the 1,300 to 1,400 nm wavelength range. Relative to the bulk scattering coefficient, the variability on the scattering anisotropy factor was found to be limited. This is because the milk scattering anisotropy is nearly independent of the fat globule and casein micelle quantity, while it is mainly determined by the size of the fat globules. As this study shows high correlations between the sample's bulk optical properties and the milk composition and fat globule size, a

  4. Short communication: Bulk milk somatic cell penalties in herds enrolled in Dairy Herd Improvement programs.

    Science.gov (United States)

    Hand, K J; Godkin, M A; Kelton, D F

    2012-01-01

    The objective of this study was to determine the effect of somatic cell count (SCC) monitoring at the cow level through Dairy Herd Improvement (DHI) programs on the risk of bulk tank SCC (BTSCC) penalties. For the year 2009, BTSCC for all producers in Ontario were examined, for a total of 2,898 DHI herds, 1,186 non-DHI herds, and 48,250 BTSCC records. Two penalty levels were examined, where BTSCC exceeded 499,000 (P500) and 399,000 (P400) cells/mL. Data were modeled first to determine the odds of a BTSCC exceeding a set penalty threshold and second to determine the odds of incurring a penalty under the Ontario Milk Act. All data were modeled as a generalized mixed model with a binary link function. Random effects included herd, fixed effects included season of BTSCC (summer, May to September, and winter, October to April), total milk shipped per month (L), fat paid per month (kg), protein paid per month (kg), and participation or not in the DHI program. The likelihood of a BTSCC exceeding a penalty threshold in a non-DHI herd compared with a DHI herd was significantly greater than 1 at both penalty levels, where the odds ratios were estimated to be 1.42 [95% confidence interval (CI): 1.19 to 1.69] and 1.38 (95% CI: 1.25 to 1.54) for P500 and P400, respectively. The likelihood of incurring a BTSCC penalty (where 3 out of 4 consecutive BTSCC exceeded penalty thresholds) was not significantly different at P500; however, it was significantly different for P400, where the odds ratio was estimated to be 1.42 (95% CI: 1.12 to 1.81).

  5. The milk delivery chain and presence of Brucella spp. antibodies in bulk milk in Uganda.

    Science.gov (United States)

    Rock, Kim Toeroek; Mugizi, Denis Rwabiita; Ståhl, Karl; Magnusson, Ulf; Boqvist, Sofia

    2016-06-01

    This study examined the influence of informal milk delivery chains on the risk of human exposure to Brucella spp. through milk consumption in two regions of Uganda (Gulu and Soroti Districts). The work involved describing milk delivery chains, investigating brucellosis awareness amongst milk deliverers and determining the presence of Brucella spp. antibodies in cattle milk on delivery to primary collection points (boiling points and dairies). Milk samples (n = 331) were collected from deliverers at primary collection points and from street vendors at point of sale and analysed using indirect enzyme-linked immunosorbent assay (I-ELISA). A written questionnaire was used to collect data from deliverers (n = 279) on their milk delivery chains and their brucellosis awareness. The most common delivery points in Gulu District were small dairies and in Soroti District boiling points. The presence of Brucella spp. antibodies in milk samples was higher in Soroti (40 %) than in Gulu (11 %) (P risk consequences of this finding as 42 % of deliverers in Soroti District reported drinking raw milk, compared with 15 % in Gulu District (P chain varied depending upon supply and demand. This study provides evidence of the diversity of informal milk markets in low-income countries and of the potential public health risks of consuming unpasteurised milk. These results can be useful to those planning interventions to reduce brucellosis. PMID:27026231

  6. Comparison of bulk-tank standard plate count and somatic cell count for Wisconsin dairy farms in three size categories.

    Science.gov (United States)

    Ingham, S C; Hu, Y; Ané, C

    2011-08-01

    The objective of this study was to evaluate possible claims by advocates of small-scale dairy farming that milk from smaller Wisconsin farms is of higher quality than milk from larger Wisconsin farms. Reported bulk tank standard plate count (SPC) and somatic cell count (SCC) test results for Wisconsin dairy farms were obtained for February to December, 2008. Farms were sorted into 3 size categories using available size-tracking criteria: small (≤118 cows; 12,866 farms), large (119-713 cattle; 1,565 farms), and confined animal feeding operations (≥714 cattle; 160 farms). Group means were calculated (group=farm size category) for the farms' minimum, median, mean, 90th percentile, and maximum SPC and SCC. Statistical analysis showed that group means for median, mean, 90th percentile, and maximum SPC and SCC were almost always significantly higher for the small farm category than for the large farm and confined animal feeding operations farm categories. With SPC and SCC as quality criteria and the 3 farm size categories of ≤118, 119 to 713, and ≥714 cattle, the claim of Wisconsin smaller farms producing higher quality milk than Wisconsin larger farms cannot be supported.

  7. Optimizing bulk milk dioxin monitoring based on costs and effectiveness

    NARCIS (Netherlands)

    Lascano Alcoser, V.; Velthuis, A.G.J.; Fels-Klerx, van der H.J.; Hoogenboom, L.A.P.; Oude Lansink, A.G.J.M.

    2013-01-01

    Dioxins are environmental pollutants, potentially present in milk products, which have negative consequences for human health and for the firms and farms involved in the dairy chain. Dioxin monitoring in feed and food has been implemented to detect their presence and estimate their levels in food ch

  8. Herd management and social variables associated with bulk tank somatic cell count in dairy herds in the eastern United States.

    Science.gov (United States)

    Schewe, R L; Kayitsinga, J; Contreras, G A; Odom, C; Coats, W A; Durst, P; Hovingh, E P; Martinez, R O; Mobley, R; Moore, S; Erskine, R J

    2015-11-01

    The ability to reduce somatic cell counts (SCC) and improve milk quality depends on the effective and consistent application of established mastitis control practices. The US dairy industry continues to rely more on nonfamily labor to perform critical tasks to maintain milk quality. Thus, it is important to understand dairy producer attitudes and beliefs relative to management practices, as well as employee performance, to advance milk quality within the changing structure of the dairy industry. To assess the adoption rate of mastitis control practices in United States dairy herds, as well as assess social variables, including attitudes toward employees relative to mastitis control, a survey was sent to 1,700 dairy farms in Michigan, Pennsylvania, and Florida in January and February of 2013. The survey included questions related to 7 major areas: sociodemographics and farm characteristics, milking proficiency, milking systems, cow environment, infected cow monitoring and treatment, farm labor, and attitudes toward mastitis and related antimicrobial use. The overall response rate was 41% (21% in Florida, 39% in Michigan, and 45% in Pennsylvania). Herd size ranged from 9 to 5,800 cows. Self-reported 3-mo geometric mean bulk tank SCC (BTSCC) for all states was 194,000 cells/mL. Multivariate analysis determined that proven mastitis control practices such as the use of internal teat sealants and blanket dry cow therapy, and not using water during udder preparation before milking, were associated with lower BTSCC. Additionally, farmer and manager beliefs and attitudes, including the perception of mastitis problems and the threshold of concern if BTSCC is above 300,000 cells/mL, were associated with BTSCC. Ensuring strict compliance with milking protocols, giving employees a financial or other penalty if BTSCC increased, and a perceived importance of reducing labor costs were negatively associated with BTSCC in farms with nonfamily employees. These findings highlight the

  9. Herd management and social variables associated with bulk tank somatic cell count in dairy herds in the eastern United States.

    Science.gov (United States)

    Schewe, R L; Kayitsinga, J; Contreras, G A; Odom, C; Coats, W A; Durst, P; Hovingh, E P; Martinez, R O; Mobley, R; Moore, S; Erskine, R J

    2015-11-01

    The ability to reduce somatic cell counts (SCC) and improve milk quality depends on the effective and consistent application of established mastitis control practices. The US dairy industry continues to rely more on nonfamily labor to perform critical tasks to maintain milk quality. Thus, it is important to understand dairy producer attitudes and beliefs relative to management practices, as well as employee performance, to advance milk quality within the changing structure of the dairy industry. To assess the adoption rate of mastitis control practices in United States dairy herds, as well as assess social variables, including attitudes toward employees relative to mastitis control, a survey was sent to 1,700 dairy farms in Michigan, Pennsylvania, and Florida in January and February of 2013. The survey included questions related to 7 major areas: sociodemographics and farm characteristics, milking proficiency, milking systems, cow environment, infected cow monitoring and treatment, farm labor, and attitudes toward mastitis and related antimicrobial use. The overall response rate was 41% (21% in Florida, 39% in Michigan, and 45% in Pennsylvania). Herd size ranged from 9 to 5,800 cows. Self-reported 3-mo geometric mean bulk tank SCC (BTSCC) for all states was 194,000 cells/mL. Multivariate analysis determined that proven mastitis control practices such as the use of internal teat sealants and blanket dry cow therapy, and not using water during udder preparation before milking, were associated with lower BTSCC. Additionally, farmer and manager beliefs and attitudes, including the perception of mastitis problems and the threshold of concern if BTSCC is above 300,000 cells/mL, were associated with BTSCC. Ensuring strict compliance with milking protocols, giving employees a financial or other penalty if BTSCC increased, and a perceived importance of reducing labor costs were negatively associated with BTSCC in farms with nonfamily employees. These findings highlight the

  10. Seasonal and Milking-to-Milking Variations in Cow Milk Fat, Protein and Somatic Cell Counts

    OpenAIRE

    Elena Raluca PAVEL; Constantin GAVAN

    2011-01-01

    The first objective of this study was to examine milking-to-milking variations in milk fat, protein and SCC (somatic cell count). The second objective of this study was to examine variations of milk components (fat, protein and SCC) over a period of six months (April-September 2010) at Agricultural Research Development Station Simnic. A total of 128 milk samples (64 morning milking and 64 evening milking ones) from milk bulk tank commingled from 90�4 Holstein cows, were collected and analyzed...

  11. Minimizing the level of butyric acid bacteria spores in farm tank milk

    NARCIS (Netherlands)

    Vissers, M.M.M.; Driehuis, F.; Giffel, M.C.T.; Jong, de P.; Lankveld, J.M.G.

    2007-01-01

    A year-long survey of 24 dairy farms was conducted to determine the effects of farm management on the concentrations of butyric acid bacteria (BAB) spores in farm tank milk (FTM). The results were used to validate a control strategy derived from model simulations. The BAB spore concentrations were m

  12. Improving farm management by modeling the contamination of farm tank milk with butyric acid bacteria

    NARCIS (Netherlands)

    Vissers, M.M.M.; Driehuis, F.; Giffel, te M.C.; Jong, de P.; Lankveld, J.M.G.

    2006-01-01

    Control of contamination of farm tank milk (FTM) with the spore-forming butyric acid bacteria (BAB) is important to prevent the late-blowing defect in semi-hard cheeses. The risk of late blowing can be decreased via control of the contamination level of FTM with BAB. A modeling approach was applied

  13. The potential of measuring serum amyloid A in individual ewe milk and in farm bulk milk for monitoring udder health on sheep dairy farms.

    Science.gov (United States)

    Winter, Petra; Miny, Martina; Fuchs, Klemens; Baumgartner, Walter

    2006-12-01

    The aim of the study was to determine the diagnostic value of measuring serum amyloid A (SAA) concentrations in milk of individual ewes and in farm bulk milk for monitoring udder health. Udder health was calculated by examining a randomly selected group of seven flocks at each farm visit by means of California mastitis test and bacteriological examination of 5749 milk samples. SAA was determined additionally in 267 randomly selected milk samples from six flocks. Thirty-one bulk milk samples from these farms were tested for SCC and SAA levels. Subclinical infections were detected in 29.5% of samples whereas no clinical infections were observed. Intramammary infected udder halves showed significantly elevated SAA concentrations (121.3+/-25.3 microg/ml) in milk compared to the levels of healthy udder halves (8.0+/-1.9 microg/ml; pCMT scores and positive bacteriological results. Bulk milk SAA levels ranged from 18.6+/-6.7 to 37.4+/-14.1 microg/ml and showed a positive correlation with bSCC (r=0.38, p=0.018) but not with percent infected glands (r=0.022, p=0.453). This study demonstrated that SAA levels in milk can be used to detect subclinical mastitis in individual ewes whereas further investigations are needed to determine the value of measuring SAA in bulk milk for monitoring flock udder health. PMID:16677674

  14. Correlation between precipitation and geographical location of the δ2H values of the fatty acids in milk and bulk milk powder

    Science.gov (United States)

    Ehtesham, E.; Baisden, W. T.; Keller, E. D.; Hayman, A. R.; Van Hale, R.; Frew, R. D.

    2013-06-01

    Hydrogen isotope ratios (δ2H) have become a tool for food traceability and authentication of agricultural products. The principle is that the isotopic composition of the produce is influenced by environmental and biological factors and hence exhibits a spatial differentiation of δ2H. This study investigates the variation in δ2H values of New Zealand milk, both in the bulk powder and individual fatty acids extracted from milk samples from dairy factories across New Zealand. Multivariate statistical analyses were used to test for relationships between δ2H of bulk milk powder, milk fatty acid and geographical location. Milk powder samples from different regions of New Zealand were found to exhibit patterns in isotopic composition similar to the corresponding regional precipitation associated with their origin. A model of δ2H in precipitation was developed based on measurements between 2007 and 2010 at 51 stations across New Zealand (Frew and Van Hale, 2011). The model uses multiple linear regressions to predict daily δ2H from 2 geographic and 5 rain-weighted climate variables from the 5 × 5 km New Zealand Virtual Climate Station Network (VCSN). To approximate collection radius for a drying facility the modelled values were aggregated within a 50 km radius of each dairy factory and compared to observed δ2H values of precipitation and bulk milk powder. Daily δ2H predictions for the period from August to December for the area surrounding the sample collection sites were highly correlated with the δ2H values of bulk milk powder. Therefore the δ2H value of milk fatty acids demonstrates promise as a tool for determining the provenance of milk powders and products where milk powder is an ingredient. Separation of milk powder origin to geographic sub-regions within New Zealand was achieved. Hydrogen isotope measurements could be used to complement traditional tracking systems in verifying point of origin.

  15. Short communication: Prevalence of methicillin resistance in coagulase-negative staphylococci and Staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Belomestnykh, N; Gamroth, M; Ruegg, P L; Tikofsky, L; Schukken, Y H

    2014-05-01

    The objective of this study was to evaluate the presence of methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in New York, Wisconsin, and Oregon from March 2009 to May 2011. Due to recent publications reporting the presence mecC (a mecA homolog not detected by traditional mecA-based PCR methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery of methicillin-resistant organisms from bulk tank milk. In total, 13 isolates were identified as methicillin resistant: Staph. aureus (n=1), Staphylococcus sciuri (n=5), Staphylococcus chromogenes (n=2), Staphylococcus saprophyticus (n=3), Staphylococcus agnetis (n=1), and Macrococcus caseolyticus (n=1). The single methicillin-resistant Staph. aureus isolate was identified from an organic farm in New York, for an observed 0.3% prevalence at the farm level. The methicillin-resistant coagulase-negative staphylococci prevalence was 2% in the organic population and 5% in the conventional population. We did not identify mecC in any of the isolates from our population. Of interest was the relatively high number of methicillin-resistant Staph. sciuri recovered, as the number of isolates from our study was considerably higher than those recovered from other recent studies that also assessed milk samples. Our research suggests that the presence of a potential methicillin-resistant Staphylococcus reservoir in milk, and likely the dairy farm population in the United States, is independent of the organic or conventional production system.

  16. Short communication: Prevalence of methicillin resistance in coagulase-negative staphylococci and Staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Belomestnykh, N; Gamroth, M; Ruegg, P L; Tikofsky, L; Schukken, Y H

    2014-05-01

    The objective of this study was to evaluate the presence of methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in New York, Wisconsin, and Oregon from March 2009 to May 2011. Due to recent publications reporting the presence mecC (a mecA homolog not detected by traditional mecA-based PCR methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery of methicillin-resistant organisms from bulk tank milk. In total, 13 isolates were identified as methicillin resistant: Staph. aureus (n=1), Staphylococcus sciuri (n=5), Staphylococcus chromogenes (n=2), Staphylococcus saprophyticus (n=3), Staphylococcus agnetis (n=1), and Macrococcus caseolyticus (n=1). The single methicillin-resistant Staph. aureus isolate was identified from an organic farm in New York, for an observed 0.3% prevalence at the farm level. The methicillin-resistant coagulase-negative staphylococci prevalence was 2% in the organic population and 5% in the conventional population. We did not identify mecC in any of the isolates from our population. Of interest was the relatively high number of methicillin-resistant Staph. sciuri recovered, as the number of isolates from our study was considerably higher than those recovered from other recent studies that also assessed milk samples. Our research suggests that the presence of a potential methicillin-resistant Staphylococcus reservoir in milk, and likely the dairy farm population in the United States, is independent of the organic or conventional production system. PMID:24582450

  17. Risk factors for bulk milk somatic cell counts and total bacterial counts in smallholder dairy farms in the 10th region of Chile.

    Science.gov (United States)

    van Schaik, G; Green, L E; Guzmán, D; Esparza, H; Tadich, N

    2005-01-01

    We investigated the principal management factors that influenced bulk milk somatic cell count (BMSCC) and total bacterial count (TBC) of smallholder dairy farms in the 10th region of Chile. One hundred and fifty smallholder milk producers were selected randomly from 42 milk collection centres (MCCs). In April and May of 2002, all farms were visited and a detailed interview questionnaire on dairy-cow management related to milk quality was conducted. In addition, the BMSCC and TBC results from the previous 2 months' fortnightly tests were obtained from the MCCs. The mean BMSCC and TBC were used as the dependent variables in the analyses and were normalised by a natural-logarithm transformation (LN). All independent management variables were categorised into binary outcomes and present (=1) was compared with absent (=0). Biserial correlations were calculated between the LNBMSCC or LNTBC and the management factors of the smallholder farms. Management factors with correlations with P0.05) factors. A random MCC effect was included in the models to investigate the importance of clustering of herds within MCC. In the null model for mean LNTBC, the random effect of MCCs was highly significant. It was explained by: milk collected once a day or less compared with collection twice a day, not cleaning the bucket after milking mastitic cows versus cleaning the bucket and cooling milk in a vat of water versus not cooling milk or using ice or a bulk tank to cool milk. Other factors that increased the LNTBC were a waiting yard with a soil or gravel floor versus concrete, use of plastic buckets for milking instead of metal, not feeding California mastitis test (CMT)-positive milk to calves and cows of dual-purpose breed. The final model explained 35% of the variance. The model predicted that a herd that complied with all the management practices had a mean predicted TBC of 105 colony forming units (cfu)/ml, whereas a herd that did not comply with any of these management factors had

  18. Microbiological quality and somatic cell count in bulk milk of dromedary camels (Camelus dromedarius): descriptive statistics, correlations, and factors of variation.

    Science.gov (United States)

    Nagy, P; Faye, B; Marko, O; Thomas, S; Wernery, U; Juhasz, J

    2013-09-01

    The objectives of the present study were to monitor the microbiological quality and somatic cell count (SCC) of bulk tank milk at the world's first large-scale camel dairy farm for a 2-yr period, to compare the results of 2 methods for the enumeration of SCC, to evaluate correlation among milk quality indicators, and to determine the effect of specific factors (year, season, stage of lactation, and level of production) on milk quality indicators. The study was conducted from January 2008 to January 2010. Total viable count (TVC), coliform count (CC), California Mastitis Test (CMT) score, and SCC were determined from daily bulk milk samples. Somatic cell count was measured by using a direct microscopic method and with an automatic cell counter. In addition, production parameters [total daily milk production (TDM, kg), number of milking camels (NMC), average milk per camel (AMC, kg)] and stage of lactation (average postpartum days, PPD) were recorded for each test day. A strong correlation (r=0.33) was found between the 2 methods for SCC enumeration; however, values derived using the microscopic method were higher. The geometric means of SCC and TVC were 394×10(3) cells/mL and 5,157 cfu/mL during the observation period, respectively. Somatic cell count was >500×10(3) cells/mL on 14.6% (106/725) and TVC was >10×10(3) cfu/mL on 4.0% (30/742) of the test days. Both milk quality indicators had a distinct seasonal pattern. For log SCC, the mean was lowest in summer and highest in autumn. The seasonal pattern of log TVC was slightly different, with the lowest values being recorded during the spring. The monthly mean TVC pattern showed a clear difference between years. Coliform count was CMT score (r=0.26), and between log TVC and CC in yr 1 (r=0.30). All production parameters and stage of lactation showed strong seasonal variation. Log SCC was negatively correlated with TDM (r=-0.35), AMC (r=-0.37), and NMC (r=-0.15) and positively correlated with PPD (r=0.40). Log TVC

  19. Performance evaluation of bulk freeze dried starter cultures of dahi and yoghurt along with probiotic strains in standardized milk of cow and buffalo

    OpenAIRE

    Vijayendra, S. V. N.; Gupta, R. C.

    2013-01-01

    Performance of bulk freeze dried (BFD) cultures of dahi (D) and yoghurt (Y) either with or without probiotic cultures (AB -Lactobacillus acidophilus and Bifidobacterium bifidum) in standardized milk of cow and buffalo was evaluated. In buffalo milk, significantly (p 

  20. Efficiency of cleaning procedure of milking equipment and bacterial quality of milk

    Directory of Open Access Journals (Sweden)

    Anna Sandrucci

    2010-01-01

    Full Text Available The cleaning and sanitation of milking equipment could be consider a critical point in the milking procedure because a cleaning failure could influence the level of bacteria contamination of bulk tank milk. Aim of the study is to monitor the cleaning procedures of milking systems in 7 dairy cows farms in Lombardy and to find a relation between efficacy of cleaning system and the bacterial quality of bulk tank milk, remaining washing water through milking equipment and teat cup surface. Cleaning procedures were monitored with Lactocorder, that measured: duration, water temperature, turbulence, percentage of water in pipes, water conductivity of pre- and post-rinse and detergent phases. Results showed that the monitored farms the most of the cleaning parameters were lower than recommendations, in particular maximum water temperature (42.1±9.9°C and percentage of water during detergent phase (76.1±13.9 %. A maximum temperature of detergent phase <40°C determined a high Standard Plate Count (SPC, thermoduric bacteria and Coliform Count (CC of bulk tank milk, SPC and CC of teat cup surface. The research indicated that monitoring the efficiency of cleaning milking equipment with proper tools provide useful information about possible sources of contamination of bulk tank milk. Improving cleaning milking efficiency allow to improve milk quality.

  1. Effects of a single prolonged milking interval in cows

    OpenAIRE

    Lakic, Branislav

    2011-01-01

    A single prolonged milking interval (PMI), such as might be associated with technical failures in automatic milking systems, leads to a subsequent somatic cell count (SCC) peak in bulk tank milk. Increased SCC indicates mastitis in cows. It is generally correlated with reduced milk yield and quality, and is often used as a criterion for milk payment. Whether the transient SCC peak after a single PMI affects yield and quality is not known. The short duration of the inflammatory ...

  2. Technical Schemes and Characteristics of Water-cooling Milk Tanks%水冷式奶罐的技术方案及其特性分析

    Institute of Scientific and Technical Information of China (English)

    田雅颂; 陈东; 谢继红; 李国盛

    2015-01-01

    Direct cooling milk tanks need high power and energy consumption, so four technical schemes of water-cooling milk tanks are put forward: water-cooling milk tank using groundwater, water-cooling milk tank using outdoor air, water-cooling milk tank with ice storage and water-cooling milk tank producing hot water. Working principle and characteristics of four technical schemes are introduced and compared based on 10 tons of milk tanks. It shows that compared with direct cooling milk tanks, equipment cost and electricity consumption of water-cooling milk tanks using groundwater is about half of that, water-cooling milk tanks using outdoor air can decrease electricity consumption cost more than 30%, electricity consumption cost of water-cooling milk tanks with ice storage can decrease more than 50%, water-cooling milk tanks preparing hot water can get 1~3 tons hot water of 40~80℃ to meet needs of cleaning milking equipment and milk tanks.%针对直冷式奶罐功率配置较高、能耗较大等不足,给出了4种水冷式奶罐技术方案:采用地下水冷源的水冷式奶罐、采用室外空气冷源的水冷式奶罐、采用冰蓄冷的水冷式奶罐和同时制取热水的水冷式奶罐。对4种技术方案的工作流程和特点进行了介绍,并以10吨奶罐为例,对4种水冷式奶罐和直冷式奶罐进行了计算比较。结果表明:与直冷式奶罐相比,采用地下水冷源的水冷式奶罐设备费用和电耗费用均降低约50%;采用室外空气冷源的水冷式奶罐电耗费用可降低30%以上;采用冰蓄冷的水冷式奶罐耗电费用降低约50%;同时制取热水的水冷式奶罐可免费获得1~3吨40~80℃的热水,可满足挤奶装置和奶罐的清洗需要。

  3. Short communication: Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count.

    Science.gov (United States)

    Koop, G; Dik, N; Nielen, M; Lipman, L J A

    2010-06-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms, 3 bulk milk samples were collected at intervals of 2 wk. The samples were cultured for SPC, coliform count, and staphylococcal count and for the presence of Staphylococcus aureus. Furthermore, SCC (Fossomatic 5000, Foss, Hillerød, Denmark) and TBC (BactoScan FC 150, Foss) were measured. Staphylococcal count was correlated to SCC (r=0.40), TBC (r=0.51), and SPC (r=0.53). Coliform count was correlated to TBC (r=0.33), but not to any of the other variables. Staphylococcus aureus did not correlate to SCC. The contribution of the staphylococcal count to the SPC was 31%, whereas the coliform count comprised only 1% of the SPC. The agreement of the repeated measurements was low. This study indicates that staphylococci in goat bulk milk are related to SCC and make a significant contribution to SPC. Because of the high variation in bacterial counts, repeated sampling is necessary to draw valid conclusions from bulk milk culturing.

  4. Effect of precipitation, geographical location and biosynthesis on New Zealand milk powder bulk and fatty acids D/H ratios

    Science.gov (United States)

    Frew, R.; Emad Ehtesham, R.; Van Hale, R.; Hayman, A.; Baisden, T.

    2012-04-01

    D/H ratio measurements provide useful information for the investigation of biogeochemical influences on natural and agricultural produce, particularly with application to food traceability and authentication. Numerous studies have shown that variation of a product's D/H ratio is influenced by both environmental factors and biological processes. This study investigates the D/H ratio of New Zealand milk powder and individual fatty acids, and causal determinants of isotopic variation. One of the key environmental factors is precipitation, and the D/H ratio "isoscaping" of NZ has been undertaken. New Zealand provides a unique geography for these kinds of study in terms of proximity to the ocean and natural geographical variability from sea level to elevations as high as 3700 m. Milk powder samples were collected from different geographical regions from milk processing units, which were supplied by producers in the immediate region. H/D ratios of bulk milk powder and of individual fatty acids were determined. Initial comparison of the precipitation and milk powder bulk D/H data show a very good differentiation from north to southernmost parts of New Zealand and a relation between rain and milk bulk D/H abundance ratio. Almost 98% of milk FAs are in the form of triglycerides that have been extracted and hydrolysed to free FAs. Free FAs were esterified and analyzed with GC-IRMS. Individual FAs show variation in D/H ratio, and all values are depleted relative to the precipitation data. The difference in D/H ratio amongst individual FAs reflects the geographical environment and biological processes i.e. micro-organisms activity in the rumen of the cow. Short chain FAs (less than 8 carbons), particularly C4 (Butyric acid), appear to be key determinants. The variation in the data can be rationalized using statistical multivariate analysis.

  5. Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil

    Directory of Open Access Journals (Sweden)

    Helena Fagundes

    2010-06-01

    Full Text Available The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3% milk samples: 14 (6.7% from samples of individual cows, and 4 (10.8% from bulk tank milk. Two individual milk samples (14.3% and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of staphylococcal food poisoning to consumers via milk of cows affected by subclinical mastitis, mainly when raw milk is ingested.

  6. Enterotoxin Gene Profile and Molecular Characterization of Staphylococcus aureus Isolates from Bovine Bulk Milk and Milk Products of Tigray Region, Northern Ethiopia.

    Science.gov (United States)

    Tarekgne, Enquebaher K; Skjerdal, Taran; Skeie, Siv; Rudi, Knut; Porcellato, Davide; Félix, Benjamin; Narvhus, Judith A

    2016-08-01

    Staphylococcal food poisoning (SFP) is an important foodborne disease worldwide, and milk and milk products are commonly associated with SFP outbreaks. The objectives of this study were to investigate the distribution of staphylococcal enterotoxin (se) genes in Staphylococcus aureus from raw cow's milk and milk products and to assess their genetic background with the spa typing method. Of the 549 samples (297 bulk milk and 162 milk product samples) collected from Tigray region, Northern Ethiopia, 160 (29.1%) were positive for S. aureus, of which 82 (51%) were found to harbor se genes by a modified multiplex PCR. Nine se genes were identified: sea (n = 12), seb (n = 3), sec (n = 3), sed(n = 4), seg (n = 49), seh (n = 2), sei (n = 40), sej (n = 1), and tsst-1 (n = 24). The classical type of genes accounted for 27%. Of the 82 enterotoxigenic isolates, 41.5 and 12.4% harbored two or more se genes, respectively. The highest gene association was observed between sei and seg, whereas sea and seb were always found together with the new types of se genes. Altogether, 18 genotypes of toxin genes were identified, and 33% of the samples contained > 5 log CFU ml(-1) S. aureus. spa typing identified 22 spa types and three novel spa sequences, which showed the high genetic diversity of the isolates. No apparent relationship was observed between spa type and se genes. Of the 25 spa types, 13 (52%) were from raw milk, 3 (12%) from milk products, and 9 (36%) from both types of sample. Types t314 (20.7%,n = 17), t458 (18.3%, n = 15), and t6218 (9.8%, n= 8) were the most common spa types identified and were widely distributed in three of the eight studied localities. This is the first study from the Tigray region to report the high distribution of enterotoxigenic S. aureus with a diversified genetic background from dairy food. The study may provide valuable data for microbial food safety risk assessment, molecular epidemiology, and phylogenetic studies of S. aureus in Ethiopia.

  7. Enterotoxin Gene Profile and Molecular Characterization of Staphylococcus aureus Isolates from Bovine Bulk Milk and Milk Products of Tigray Region, Northern Ethiopia.

    Science.gov (United States)

    Tarekgne, Enquebaher K; Skjerdal, Taran; Skeie, Siv; Rudi, Knut; Porcellato, Davide; Félix, Benjamin; Narvhus, Judith A

    2016-08-01

    Staphylococcal food poisoning (SFP) is an important foodborne disease worldwide, and milk and milk products are commonly associated with SFP outbreaks. The objectives of this study were to investigate the distribution of staphylococcal enterotoxin (se) genes in Staphylococcus aureus from raw cow's milk and milk products and to assess their genetic background with the spa typing method. Of the 549 samples (297 bulk milk and 162 milk product samples) collected from Tigray region, Northern Ethiopia, 160 (29.1%) were positive for S. aureus, of which 82 (51%) were found to harbor se genes by a modified multiplex PCR. Nine se genes were identified: sea (n = 12), seb (n = 3), sec (n = 3), sed(n = 4), seg (n = 49), seh (n = 2), sei (n = 40), sej (n = 1), and tsst-1 (n = 24). The classical type of genes accounted for 27%. Of the 82 enterotoxigenic isolates, 41.5 and 12.4% harbored two or more se genes, respectively. The highest gene association was observed between sei and seg, whereas sea and seb were always found together with the new types of se genes. Altogether, 18 genotypes of toxin genes were identified, and 33% of the samples contained > 5 log CFU ml(-1) S. aureus. spa typing identified 22 spa types and three novel spa sequences, which showed the high genetic diversity of the isolates. No apparent relationship was observed between spa type and se genes. Of the 25 spa types, 13 (52%) were from raw milk, 3 (12%) from milk products, and 9 (36%) from both types of sample. Types t314 (20.7%,n = 17), t458 (18.3%, n = 15), and t6218 (9.8%, n= 8) were the most common spa types identified and were widely distributed in three of the eight studied localities. This is the first study from the Tigray region to report the high distribution of enterotoxigenic S. aureus with a diversified genetic background from dairy food. The study may provide valuable data for microbial food safety risk assessment, molecular epidemiology, and phylogenetic studies of S. aureus in Ethiopia. PMID

  8. 40 CFR 63.11087 - What requirements must I meet for gasoline storage tanks if my facility is a bulk gasoline...

    Science.gov (United States)

    2010-07-01

    ...) If your gasoline storage tank is subject to, and complies with, the control requirements of 40 CFR... gasoline storage tanks if my facility is a bulk gasoline terminal, pipeline breakout station, or pipeline... CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for Source Category:...

  9. Exploring the characteristics and dynamics of Ontario dairy herds experiencing increases in bulk milk somatic cell count during the summer.

    Science.gov (United States)

    Shock, D A; LeBlanc, S J; Leslie, K E; Hand, K; Godkin, M A; Coe, J B; Kelton, D F

    2015-06-01

    Regionally aggregated bulk milk somatic cell count (BMSCC) data from around the world shows a repeatable cyclicity, with the highest levels experienced during warm, humid seasons. No studies have evaluated this seasonal phenomenon at the herd level. The objectives of this study were to define summer seasonality in BMSCC on an individual herd basis, and subsequently to describe the characteristics and dynamics of herds with increased BMSCC in the summer. The data used for this analysis were from all dairy farms in Ontario, Canada, between January 2000 and December 2011 (n≈4,000 to 6,000 herds/yr). Bulk milk data were obtained from the milk marketing board and consisted of bulk milk production, components (fat, protein, lactose, other solids), and quality (BMSCC, bacterial count, inhibitor presence, freezing point), total milk quota of the farm, and milk quota and incentive fill percentage. A time-series linear mixed model, with random slopes and intercepts, was constructed using sine and cosine terms as predictors to describe seasonality, with herd as a random effect. For each herd, seasonality was described with reference to 1 cosine function of variable amplitude and phase shift. The predicted months of maximal and minimal BMSCC were then calculated. Herds were assigned as low, medium, and high summer increase (LSI, MSI, and HSI, respectively) based on percentiles of amplitude in BMSCC change for each of the 4 seasons. Using these seasonality classifications, 2 transitional repeated measures logistic regression models were built to assess the characteristics of MSI and HSI herds, using LSI herds as controls. Based on the analyses performed, a history of summer BMSCC increases increased the odds of experiencing a subsequent increase. As herd size decreased, the odds of experiencing HSI to MSI in BMSCC increased. Herds with more variability in daily BMSCC were at higher odds of experiencing MSI and HSI in BMSCC, as were herds with lower annual mean BMSCC. Finally

  10. Exploring the characteristics and dynamics of Ontario dairy herds experiencing increases in bulk milk somatic cell count during the summer.

    Science.gov (United States)

    Shock, D A; LeBlanc, S J; Leslie, K E; Hand, K; Godkin, M A; Coe, J B; Kelton, D F

    2015-06-01

    Regionally aggregated bulk milk somatic cell count (BMSCC) data from around the world shows a repeatable cyclicity, with the highest levels experienced during warm, humid seasons. No studies have evaluated this seasonal phenomenon at the herd level. The objectives of this study were to define summer seasonality in BMSCC on an individual herd basis, and subsequently to describe the characteristics and dynamics of herds with increased BMSCC in the summer. The data used for this analysis were from all dairy farms in Ontario, Canada, between January 2000 and December 2011 (n≈4,000 to 6,000 herds/yr). Bulk milk data were obtained from the milk marketing board and consisted of bulk milk production, components (fat, protein, lactose, other solids), and quality (BMSCC, bacterial count, inhibitor presence, freezing point), total milk quota of the farm, and milk quota and incentive fill percentage. A time-series linear mixed model, with random slopes and intercepts, was constructed using sine and cosine terms as predictors to describe seasonality, with herd as a random effect. For each herd, seasonality was described with reference to 1 cosine function of variable amplitude and phase shift. The predicted months of maximal and minimal BMSCC were then calculated. Herds were assigned as low, medium, and high summer increase (LSI, MSI, and HSI, respectively) based on percentiles of amplitude in BMSCC change for each of the 4 seasons. Using these seasonality classifications, 2 transitional repeated measures logistic regression models were built to assess the characteristics of MSI and HSI herds, using LSI herds as controls. Based on the analyses performed, a history of summer BMSCC increases increased the odds of experiencing a subsequent increase. As herd size decreased, the odds of experiencing HSI to MSI in BMSCC increased. Herds with more variability in daily BMSCC were at higher odds of experiencing MSI and HSI in BMSCC, as were herds with lower annual mean BMSCC. Finally

  11. A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk

    DEFF Research Database (Denmark)

    Holm, C.; Mathiasen, T.; Jespersen, Lene

    2004-01-01

    were defined: region 1 includes bacteria mainly associated with poor hygiene, region 2 includes psychrotrophic hygiene bacteria and region 3 includes bacteria mainly related to mastitis. The ability of the flow cytometric technique to predict the main cause of elevated bacterial counts on routine...

  12. Phytoestrogens and Their Metabolites in Bulk-Tank Milk: Effects of Farm Management and Season

    DEFF Research Database (Denmark)

    Adler, Steffen A; Purup, Stig; Hansen-Møller, Jens;

    2015-01-01

    Phytoestrogens have structures similar to endogenous steroids and may induce or inhibit the response of hormone receptors. The objectives of the present study were to compare the effects of long-term vs. short-term grassland management in organic and conventional dairy production systems, compare...

  13. RENEWABLE ENERGY-POWERED BULK MILK COOLING FOR SMALLHOLDER DAIRY FARMERS

    Science.gov (United States)

    We will show that the cooler can successfully add the evening milk to the cold chain and that it’s wide spread use has potential to reduce the amount of methane released into the atmospheres from smallholder dairy farms.

  14. A wireless electronic monitoring system for securing milk from farm to processor

    Science.gov (United States)

    Womble, Phillip; Hopper, Lindsay; Thompson, Chris; Alexander, Suraj M.; Crist, William; Payne, Fred; Stombaugh, Tim; Paschal, Jon; Moore, Ryan; Luck, Brian; Tabayehnejab, Nasrin

    2008-04-01

    The Department of Homeland Security and the Department of Health and Human Services have targeted bulk food contamination as a focus for attention. The contamination of bulk food poses a high consequence threat to our society. Milk transport falls into three of the 17 targeted NIPP (National Infrastructure Protection Plan) sectors including agriculture-food, public health, and commercial facilities. Minimal security safeguards have been developed for bulk milk transport. The current manual methods of securing milk are paper intensive and prone to errors. The bulk milk transportation sector requires a security enhancement that will both reduce recording errors and enable normal transport activities to occur while providing security against unauthorized access. Milk transportation companies currently use voluntary seal programs that utilize plastic, numbered seals on milk transport tank openings. Our group has developed a Milk Transport Security System which is an electromechanical access control and communication system that assures the secure transport of milk, milk samples, milk data, and security data between locations and specifically between dairy farms, transfer stations, receiving stations, and milk plants. It includes a security monitoring system installed on the milk transport tank, a hand held device, optional printers, data server, and security evaluation software. The system operates automatically and requires minimal or no attention by the bulk milk hauler/sampler. The system is compatible with existing milk transport infrastructure, and has the support of the milk producers, milk transportation companies, milk marketing agencies, and dairy processors. The security protocol developed is applicable for transport of other bulk foods both nationally and internationally. This system adds significantly to the national security infrastructure for bulk food transport. We are currently demonstrating the system in central Kentucky and will report on the results

  15. TECHNICAL ASSESSMENT OF BULK VITRIFICATION PROCESS & PRODUCT FOR TANK WASTE TREATMENT AT THE DEPARTMENT OF ENERGY HANFORD SITE

    Energy Technology Data Exchange (ETDEWEB)

    SCHAUS, P.S.

    2006-07-21

    At the U.S. Department of Energy (DOE) Hanford Site, the Waste Treatment Plant (WTP) is being constructed to immobilize both high-level waste (IUW) for disposal in a national repository and low-activity waste (LAW) for onsite, near-surface disposal. The schedule-controlling step for the WTP Project is vitrification of the large volume of LAW, current capacity of the WTP (as planned) would require 50 years to treat the Hanford tank waste, if the entire LAW volume were to be processed through the WTP. To reduce the time and cost for treatment of Hanford Tank Waste, and as required by the Tank Waste Remediation System Environmental Impact Statement Record of Decision and the Hanford Federal Facility Consent Agreement (Tn-Party Agreement), DOE plans to supplement the LAW treatment capacity of the WTP. Since 2002, DOE, in cooperation with the Environmental Protection Agency and State of Washington Department of Ecology has been evaluating technologies that could provide safe and effective supplemental treatment of LAW. Current efforts at Hanford are intended to provide additional information to aid a joint agency decision on which technology will be used to supplement the WTP. A Research, Development and Demonstration permit has been issued by the State of Washington to build and (for a limited time) operate a Demonstration Bulk Vitrification System (DBVS) facility to provide information for the decision on a supplemental treatment technology for up to 50% of the LAW. In the Bulk Vitrification (BV) process, LAW, soil, and glass-forming chemicals are mixed, dried, and placed in a refractory-lined box, Electric current, supplied through two graphite electrodes in the box, melts the waste feed, producing a durable glass waste-form. Although recent modifications to the process have resulted in significant improvements, there are continuing technical concerns.

  16. TECHNICAL ASSESSMENT OF BULK VITRIFICATION PROCESS/ PRODUCT FOR TANK WASTE TREATMENT AT THE DEPARTMENT OF ENERGY HANFORD SITE

    International Nuclear Information System (INIS)

    At the U.S. Department of Energy (DOE) Hanford Site, the Waste Treatment Plant (WTP) is being constructed to immobilize both high-level waste (IUW) for disposal in a national repository and low-activity waste (LAW) for onsite, near-surface disposal. The schedule-controlling step for the WTP Project is vitrification of the large volume of LAW, current capacity of the WTP (as planned) would require 50 years to treat the Hanford tank waste, if the entire LAW volume were to be processed through the WTP. To reduce the time and cost for treatment of Hanford Tank Waste, and as required by the Tank Waste Remediation System Environmental Impact Statement Record of Decision and the Hanford Federal Facility Consent Agreement (Tn-Party Agreement), DOE plans to supplement the LAW treatment capacity of the WTP. Since 2002, DOE, in cooperation with the Environmental Protection Agency and State of Washington Department of Ecology has been evaluating technologies that could provide safe and effective supplemental treatment of LAW. Current efforts at Hanford are intended to provide additional information to aid a joint agency decision on which technology will be used to supplement the WTP. A Research, Development and Demonstration permit has been issued by the State of Washington to build and (for a limited time) operate a Demonstration Bulk Vitrification System (DBVS) facility to provide information for the decision on a supplemental treatment technology for up to 50% of the LAW. In the Bulk Vitrification (BV) process, LAW, soil, and glass-forming chemicals are mixed, dried, and placed in a refractory-lined box, Electric current, supplied through two graphite electrodes in the box, melts the waste feed, producing a durable glass waste-form. Although recent modifications to the process have resulted in significant improvements, there are continuing technical concerns

  17. DESIGN OF THE DEMOSNTRATION BULK VITRIFICATION SYSTEM FOR THE SUPPLEMENTAL TREATMENT OF LOW ACTIVITY TANK WASTE AT HANFORD

    International Nuclear Information System (INIS)

    In June 2004, the Demonstration Bulk Vitrification System (DBVS) was initiated with the intent to design, construct, and operate a full-scale bulk vitrification pilot-plant to treat low-activity tank waste from Hanford Tank 241-S-109. The DBVS facility uses In-Container Vitrification(trademark) (ICV(trademark)) at the core of the treatment process. The basic process steps combine liquid low-activity waste (LAW) and glassformers; dry the mixture; and then vitrify the mixture in a batch feed-while-melt process in a refractory lined steel container. Off-gases are processed through a state-of-the-art air pollution control system including sintered-metal filtration, thermal oxidation, acid gas scrubbing, and high-efficiency particulate air (HEPA) and high-efficiency gas adsorber (HEGA) filtration. Testing has focused on development and validation of the waste dryer, ICV, and sintered-metal filters (SMFs) equipment, operations enhancements, and glass formulation. With a parallel testing and design process, testing has allowed improvements to the DBVS equipment configuration and operating methodology, since its original inception. Design improvements include optimization of refractory panels in the ICV, simplifying glassformer addition equipment, increasing the number of waste feed chutes to the ICV, and adding capability for remote clean-out of piping, In addition, the U.S. Department of Energy (DOE) has provided an independent review of the entire DBVS process. While the review did not find any fatal flaws, some technical issues were identified that required a re-evaluation of the DBVS design and subsequent changes to the design. A 100 percent design package for the pilot plant will be completed and submitted to DOE for review in early 2008 that incorporates process improvements substantiated through testing and reviews. This paper provides a description of the bulk vitrification process and a discussion of major equipment design changes that have occurred based on full

  18. A survey on Aflatoxin M1 content in sheep and goat milk produced in Sardinia Region, Italy (2005-2013)

    OpenAIRE

    Salvatore Virdis; Christian Scarano; Vincenzo Spanu; Gavino Murittu; Carlo Spanu; Ignazio Ibba; Enrico Pietro Luigi De Santis

    2014-01-01

    In the present work the results of a survey conducted in Sardinia Region on Aflatoxin M1 (AFM1) contamination in milk of small ruminants from 2005 to 2013 are reported. A total of 517 sheep and 88 goat milk samples from bulk tank, tank trucks and silo tank milk were collected. Analyses were performed by the Regional Farmers Association laboratory using high-performance liquid chromatography following the ISO 14501:1998 standard. None of the sheep milk samples analysed during 2005- 2012 showed...

  19. Dismantlement and removal of Old Hydrofracture Facility bulk storage bins and water tank, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The Old Hydrofracture Facility (OHF), located at Oak Ridge National Laboratory (ORNL), was constructed in 1963 to allow experimentation and operations with an integrated solid storage, mixing, and grout injection facility. During its operation, OHF blended liquid low-level waste with grout and used a hydrofracture process to pump the waste into a deep low-permeable shale formation. Since the OHF Facility was taken out of service in 1980, the four bulk storage bins located adjacent to Building 7852 had deteriorated to the point that they were a serious safety hazard. The ORNL Surveillance and Maintenance Program requested and received permission from the US Department of Energy to dismantle the bins as a maintenance action and send the free-released metal to an approved scrap metal vendor. A 25,000-gal stainless steel water tank located at the OHF site was included in the scope. A fixed-price subcontract was signed with Allied Technology Group, Inc., to remove the four bulk storage bins and water tank to a staging area where certified Health Physics personnel could survey, segregate, package, and send the radiologically clean scrap metal to an approved scrap metal vendor. All radiologically contaminated metal and metal that could not be surveyed was packaged and staged for later disposal. Permissible personnel exposure limits were not exceeded, no injuries were incurred, and no health and safety violations occurred throughout the duration of the project. Upon completion of the dismantlement, the project had generated 53,660 lb of clean scrap metal (see Appendix D). This resulted in $3,410 of revenue generated and a cost avoidance of an estimated $100,000 in waste disposal fees.

  20. How are flexible tanks becoming a solution for wine in bulk transportation?

    OpenAIRE

    Aaltonen, Alisa

    2015-01-01

    Past decades have proven that logistics is taking a crucial role in both domestic and international trade. Additionally, logistics have played an essential role in wars recorded in past human history. Bulk shipping is becoming an increasingly attractive option for wine shippers around the world because this method of shipping allows transporting up to 13,400 litres more of the product when compared to the conventional method of wine transportation, which dramatically cuts transportation costs...

  1. Prevalence and seasonality of bulk milk antibodies against Dictyocaulus viviparus and Ostertagia ostertagi in Irish pasture-based dairy herds.

    Science.gov (United States)

    Bloemhoff, Yris; Forbes, Andrew; Good, Barbara; Morgan, Eric; Mulcahy, Grace; Strube, Christina; Sayers, Ríona

    2015-04-15

    Infections with Dictyocaulus viviparus and Ostertagia ostertagi nematode parasites are of importance to bovine health and production in temperate areas across the world. Losses due to these parasites in dairy herds can be considerable due to decreased milk productivity and fertility. However, information on current epidemiological patterns in Irish dairy herds is limited. Bulk milk samples were collected from a total of 319 dairy farms across the Republic of Ireland. The D. viviparus samples were tested with an ELISA based on recombinant major sperm protein, while the O. ostertagi samples were tested with an ELISA based on crude saline extract, whole worm O. ostertagi antigen. Management data were collected from the farms using a questionnaire. Logistic regression was used to find significant associations between the presence of antibodies against D. viviparus and O. ostertagi and management factors. The overall prevalence of D. viviparus infection was 62.8%, while over 98% of herds had antibodies to O. ostertagi at the specified cut-off. Both D. viviparus and O. ostertagi antibodies were highest in November, which could be explained by the accumulated uptake of larvae through the grazing season. In herds of farmers that dosed their in-calf heifers with anthelmintics were significantly more likely to be positive for antibodies against D. viviparus infection. This study highlights that both D. viviparus and O. ostertagi infections are widespread in dairy herds in Ireland throughout the grazing season. PMID:25709092

  2. A Heuristic and Hybrid Method for the Tank Allocation Problem in Maritime Bulk Shipping

    DEFF Research Database (Denmark)

    Vilhelmsen, Charlotte; Larsen, Jesper; Lusby, Richard Martin

    and strength as well as other operational constraints. The problem of finding a feasible solution to this tank allocation problem has been shown to be NP-Complete. We approach the problem on a tactical level where requirements for computation time are strict while solution quality is less important than simply...... have created a hybrid method that first runs the heuristic and if the heuristic fails to solve the problem, then runs the modified optimality based method on the parts of the problem that the heuristic did not solve. This hybrid method cuts between 90% and 94% of the average running times compared...... finding a feasible solution. We have developed a heuristic that can efficiently find feasible cargo allocations. Computational results show that it can solve 99% of the considered instances within 0.4 seconds and all of them if allowed longer time. We have also modified an optimality based method from...

  3. A heuristic and hybrid method for the tank allocation problem in maritime bulk shipping

    DEFF Research Database (Denmark)

    Vilhelmsen, Charlotte; Larsen, Jesper; Lusby, Richard Martin

    2016-01-01

    and strength as well as other operational constraints. The problem of finding a feasible solution to this tank allocation problem has been shown to be NP-Complete. We approach the problem on a tactical level where requirements for computation time are strict while solution quality is less important than simply...... have created a hybrid method that first runs the heuristic and if the heuristic fails to solve the problem, then runs the modified optimality based method on the parts of the problem that the heuristic did not solve. This hybrid method cuts between 90 and 94 % of the average running times compared...... finding a feasible solution. We have developed a heuristic that can efficiently find feasible cargo allocations. Computational results show that it can solve 99 % of the considered instances within 0.4 s and all of them if allowed longer time. We have also modified an optimality based method from...

  4. Characteristics Analysis of the Typical Technical Schemes for Milk Cooling Tanks%牛奶冷却罐典型技术方案及其特性分析

    Institute of Scientific and Technical Information of China (English)

    谢继红; 陈东; 李国盛

    2015-01-01

    Milk cooling tanks are important devices in pasturelands and milk collection stations. Three typical technical schemes are presented: direct-cooling milk tank, water-cooling milk tank and rapid-cooling milk tank. The basic structures and working principles of these tanks are introduced. The power matching and energy consumption are calculated and analyzed for the three types of 6-ton milk tank, and it is shown that the rapid-cooling milk tank has more comprehensive advantages.%牛奶冷却罐是牧场和牛奶收集站的主要设备。给出了牛奶冷却罐三个典型的技术方案,分别为直冷式奶罐、水冷式奶罐和速冷式奶罐。对不同奶罐的基本构成、工作原理进行了介绍,并以6t奶罐为例,对不同奶罐的功率匹配和能耗进行了计算分析。结果表明,速冷式奶罐具有较好的综合优势。

  5. A Survey on aflatoxin M1 content in sheep and goat milk produced in Sardinia region, Italy (2005-2013)

    OpenAIRE

    Virdis, Salvatore; Scarano, Christian; Spanu, Vincenzo; Murittu, Gavino; Spanu, Carlo; Ibba, Ignazio; De Santis, Enrico Pietro Luigi

    2014-01-01

    In the present work the results of a survey conducted in Sardinia Region on Aflatoxin M1 (AFM1) contamination in milk of small ruminants from 2005 to 2013 are reported. A total of 517 sheep and 88 goat milk samples from bulk tank, tank trucks and silo tank milk were collected. Analyses were performed by the Regional Farmers Association laboratory using high-performance liquid chromatography following the ISO 14501:1998 standard. None of the sheep milk samples analysed during 2005- 2012 showed...

  6. Quality based payment program and milk quality in dairy cooperatives of Southern Brazil: an econometric analysis

    Directory of Open Access Journals (Sweden)

    Bruno Garcia Botaro

    2013-02-01

    Full Text Available Programs designed to enhance milk quality have been used to motivate dairy farmers to improve the quality of the raw milk they produce. The objective of this study was to evaluate the association between a milk quality payment program and four indicative variables of milk quality, by testing bulk tank somatic cell count (SCC, bulk tank total bacterial count (TBC, fat (FAT and protein (PROT percentages over three years in four dairy cooperatives in Southern Brazil. We used a multiple regression econometric model estimated from market data of milk delivered by farmers to the cooperatives. Bulk tank milk samples (n = 19,644 were monthly collected. The data set was analyzed for the effects of seasonality, average daily volume of milk, the award/penalty, producer, and cooperatives on SCC, TBC, FAT and PROT. Results suggested an association between the adoption of a payment program based on milk quality and the reduction of SCC and TBC. Nevertheless, the program seems to have not contributed to increase fat and protein milk percentages. This information may help the dairy industry in developing countries to conceive strategies to enhance overall milk quality.

  7. A survey on Aflatoxin M1 content in sheep and goat milk produced in Sardinia Region, Italy (2005-2013

    Directory of Open Access Journals (Sweden)

    Salvatore Virdis

    2014-12-01

    Full Text Available In the present work the results of a survey conducted in Sardinia Region on Aflatoxin M1 (AFM1 contamination in milk of small ruminants from 2005 to 2013 are reported. A total of 517 sheep and 88 goat milk samples from bulk tank, tank trucks and silo tank milk were collected. Analyses were performed by the Regional Farmers Association laboratory using high-performance liquid chromatography following the ISO 14501:1998 standard. None of the sheep milk samples analysed during 2005- 2012 showed AFM1 contamination. In sheep milk samples collected in 2013, 8 out of 172 (4.6% were contaminated by AFM1 with a concentration (mean±SD of 12.59±14.05 ng/L. In one bulk tank milk sample 58.82 ng/L AFM1 was detected, exceeding the EU limit. In none of goat milk samples analysed from 2010 to 2012 AFM1 was detected. In 2013, 9 out of 66 goat milk samples (13.6% showed an AFM1 concentration of 47.21±19.58 ng/L. Two of these samples exceeded the EU limit, with concentrations of 62.09 and 138.6 ng/L. Higher contamination frequency and concentration rates were detected in bulk tank milk samples collected at farm than in bulk milk truck or silo samples, showing a dilution effect on AFM1 milk content along small ruminants supply chain. The rate and levels of AFM1 contamination in sheep and goat milk samples were lower than other countries. However, the small number of milk samples analysed for AFM1 in Sardinia Region in 2005-2013 give evidence that food business operators check programmes should be improved to ensure an adequate monitoring of AFM1 contamination in small ruminant dairy chain.

  8. EVALUATION OF LISTERIA MONOCYTOGENES IN RAW BUFFALO MILK DURING PRIMARY PRODUCTION

    Directory of Open Access Journals (Sweden)

    M.L. Cortesi

    2009-06-01

    Full Text Available Regulation EC 852/2004 lays down rules concerning hygiene requirements and the adoption of adequate measures for identification, prevention and control of contamination risks during milking in primary production. Aim of this research was to detect Listeria monocytogenes and to check the total viable count (TVC at 30° in raw buffalo milk coming from ten dairy farms. On the whole 207 samples of raw buffalo milk were collected and analysed during one year, 189 samples were collected from single animals and 18 samples from tank-bulk milk. In raw bulk-tank milk Listeria monocytogenes was never detected. In two out five positive samples from single animals Listeria monocytogenes was detected by means of quantitative method at levels ≤1 cfu/ml.

  9. Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media.

    Science.gov (United States)

    Souto, Luís I M; Minagawa, Clarice Y; Telles, Evelise O; Garbuglio, Márcio A; Amaku, Marcos; Melville, Priscilla A; Dias, Ricardo A; Sakata, Sonia T; Benites, Nilson R

    2010-02-01

    Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count. PMID:19939321

  10. Association between antibodies to Coxiella burnetii in bulk tank milk and perinatal mortality of Danish dairy calves

    DEFF Research Database (Denmark)

    Nielsen, Katrine T.; Nielsen, Søren S.; Agger, Jens F.;

    2011-01-01

    on two outcomes associated with parturition in cattle: a) stillbirth; and b) stillbirth and neonatal mortality combined (perinatal death). Methods: Twenty-four Danish dairy herds were tested repeatedly for antibodies to C. burnetii in BTM using a commercial ELISA. Samples were collected monthly from July...

  11. Correlations among the somatic cell count of individual bulk milk, result of the California Mastitis Test and bacteriological status of the udder in dairy cows.

    Science.gov (United States)

    Jánosi, Sz; Baltay, Zs

    2004-01-01

    In a survey of about 3000 dairy cows producing low somatic cell count (SCC) milk and kept on a large-scale dairy farm, California Mastitis Test (CMT) positivity was found in 2714 udder quarters of 1491 cows. Pathogenic microorganisms were isolated from 57.6% of these 2714 udder quarters during bacteriological examination. The commonest pathogens were coagulase-negative staphylococci (CNS, 41%) and Staphylococcus aureus (32.5%); however, udder infections caused by environmental streptococci (12.8%) and coliform bacteria (6.8%) were also common. All pathogens resulted in a significant increase of the SCC in individual bulk milk (IBM) samples. In the case of CNS, this SCC elevation in IBM was significantly lower than in the case of infection by the other pathogens. In spite of this, because of the high number of udder infections caused by CNS, the adverse effect exerted by CNS on dairy herds is considered to be substantial. It was found that 54.6% of all CMT-positive cows produced IBM of an SCC below 400 thousand per ml. The milk produced by 41% of the 315 cows excreting S. aureus also had an SCC below 400 thousand per ml. This poses a serious risk of infection to the healthy herdmates. At the same time, 11% of the infected cows produced IBM with an SCC below 100 thousand per ml. On the basis of these findings, only the regular analysis of SCC of IBM can be a reliable indicator of chronic intramammary infection. As the SCC of milk produced by CMT-positive cows (and especially of those excreting pathogens) tended to increase with advancing lactation, the authors suggest that an efficient drying-off therapy should be used to restore udder health and, whenever justified, culling of cows cannot be avoided either. PMID:15168749

  12. ANALYSIS OF MILK QUALITY AND ITS IMPORTANCE FOR MILK PROCESSORS

    OpenAIRE

    AGATHA POPESCU; ELENA ANGEL

    2013-01-01

    The paper aimed to present some aspects regarding milk quality and its importance for milk processors , taking into account a study case at FLAV O’RICH DAIRY INC,USA. The study analyses how milk quality is checked from the bulk milk to final product according to the Milk Quality Program in force. The main aspects concerning raw milk selection criteria such as : antibiotic test, temperature, bacteria, organoleptic properties , acidity, somatic cell count , but also main milk components such as...

  13. Detection of Mycobacterium tuberculosis and Mycobacterium avium Complexes by Real-Time PCR in Bovine Milk from Brazilian Dairy Farms.

    Science.gov (United States)

    Bezerra, André Vinícius Andrade; Dos Reis, Emily Marques; Rodrigues, Rogério Oliveira; Cenci, Alexander; Cerva, Cristine; Mayer, Fabiana Quoos

    2015-05-01

    Foodborne diseases are a public health problem worldwide. The consumption of contaminated raw milk has been recognized as a major cause of transmission of bovine tuberculosis to humans. Other mycobacteria that may be present in raw milk and may cause diseases are those belonging to the Mycobacterium avium complex. In this study, molecular biology tools were applied to investigate raw milk contamination with Mycobacterium spp. in family dairy farms from Rio Grande do Sul, southern Brazil. Furthermore, different variables related to the source of the milk, herd characteristics, and management were evaluated for their effect on milk contamination. Five hundred and two samples were analyzed, of which 354 were from the Northwest region (102 farms with samples from 93 bulk tanks and 261 animals) and 148 from the South region of the state (22 farms with samples from 23 bulk tanks and 125 animals). Among them, 10 (1.99%) and 7 (1.39%) were positive for Mycobacterium tuberculosis (9 confirmed as Mycobacterium bovis) and M. avium complexes, respectively. There was no difference in the frequencies of positive samples between the regions or the sample sources. Of the positive samples, 4 were collected from a bulk tank (1 positive for M. avium and 3 for M. tuberculosis). Moreover, 1 sample was positive concomitantly for M. tuberculosis and M. avium complexes. On risk analysis, no variable was associated with raw milk contamination by M. tuberculosis complex species. However, washing the udders of all animals and drying them with paper towels were weakly classified as risk factors for M. avium contamination. Positive samples were obtained from both animals and bulk tanks, which emphasizes the importance of tuberculosis control programs and provides evidence that milk monitoring can be used as a control practice. Moreover, the findings of this study reinforce the need for awareness of the problems of raw milk consumption among the general population.

  14. Relations between basic milk components and free fatty acid content in Holstein cow milk as lipolysis parameter

    Directory of Open Access Journals (Sweden)

    Luděk Stádník

    2015-02-01

    Full Text Available The aim of this study was to evaluate in detail the relationship between the basic milk components (fat and protein percentage and the free fatty acids (FFA content, as indicators of spontaneous and/or induced lipolysis. The additional aim of the study was to compare the FFA content of milk with respect to spontaneous and induced lipolysis. Milking was carried out in herringbone parlour twice a day. In total, 540 milk samples were obtained for evaluation of spontaneous (n=240 and induced lipolysis (n=300. The milk samples for determination of basic milk components and FFA (li¬polysis levels were collected during four subsequent lactation weeks. Milk samples for spontaneous lipolysis detection were taken directly in parlour immediately after milking using the ICAR methodology and subsequently grouped. Induced lipolysis was observed from bulk milk in time 0, 1, 2, 3, and 4 hours after milking (T0-T4. The evaluation of basic components and FFA content was carried on MILKOSCAN F120 (Foss Electronic; Denmark. Statistical evaluation was carried out using SAS 9.3. (SAS/STAT® 9.3, 2011. An increase of one percentage of milk fat was equal to 0.318 mmol x 100 g-1 FFA decline (spontaneous lipolysis or from 0.232 to 0.370 mmol x 100 g-1 FFA decline (induced lipolysis T0-T4 respectively. One percentage increase of milk protein was equal to 1.219 mmol x 100 g-1 FFA increase (spontaneous lipolysis or 0.421 to 1.531 mmol x 100 g-1 FFA decrease (induced lipolysis T0 - T4 respectively. Significant differences (P<0.01 were detected among FFA content in relation to spontaneous and induced lipolysis evaluated during storage and cooling after milking. The minimal differences were detected between the FFA content during 4 hours cooling and storage of milk in the tank.

  15. Lead excretion in milk of accidentally exposed dairy cattle.

    Science.gov (United States)

    Bischoff, Karyn; Higgins, William; Thompson, Belinda; Ebel, Joseph G

    2014-01-01

    Lead (Pb) exposure in dairy cattle is associated with economic losses due to mortality and treatment costs, but with production animals there is also risk to the human food chain. The first objective of this study was to quantify the Pb concentration in milk from Pb-exposed cattle. The second objective was to correlate blood and milk Pb concentrations from individual cows. The third objective was long-term monitoring to determine the duration of milk contamination after exposure ceased. A dairy herd of more than 100 cows was accidentally exposed to Pb-contaminated feed. Milk and blood were collected for Pb analysis. Serial collection of milk samples continued for 2.5 years. The initial concentration of Pb in bulk tank milk was 0.0999 mg l⁻¹. The highest milk Pb concentration from an individual cow was 0.4657 mg l⁻¹ and the highest blood Pb concentration was 1.216 mg l⁻¹. One milk sample collected at the end of the study (day 922) contained 0.0117 mg Pb l⁻¹ of Pb. The calculated relationship between milk (y) and blood (x) Pb concentration was ln(y) = 3.4(x) - 2.21 (R² = 0.98).

  16. 49 CFR 178.320 - General requirements applicable to all DOT specification cargo tank motor vehicles.

    Science.gov (United States)

    2010-10-01

    ..., intermediate bulk containers, multi-unit tank car tanks, portable tanks, or tank cars. Cargo tank motor vehicle... tank to a motor vehicle or to a motor vehicle suspension component that involves welding on the...

  17. Survey on the fatty acids profile of fluid goat milk

    Directory of Open Access Journals (Sweden)

    Daniela Pittau

    2013-10-01

    Full Text Available Fluid goat milk submitted to thermal treatment has interesting nutritional properties and a potential expanding market. The present study was aimed to conduct fatty acids profile characterisation of goat milk placed on market. Forty-nine fluid milk samples were collected: 12 pasteurised, 12 pasteurised at high temperature, 11 ultrahigh temperature (UHT whole milk and 14 UHT semi-skimmed milk. Milk samples were collected at retail level from 7 different companies and from different production batches. After extraction and methilation, fatty acids (FAs profile was determined on each sample using a gas chromatograph with flame ionisation detector (GC-FID with high-polarity capillary column. The concentration (g/100mL of saturated fatty acids (SFAs, monounsaturated fatty acids (MUFAs, polyunsaturated fatty acids (PUFAs, trans fatty acids (t-FAs, and isomers of conjugated linoleic acid (CLA was determined. N-6/n-3 ratio, atherogenic index (AI and thrombogenic index (TI were also assessed. Fluid goat milk lipid profile was characterised by SFAs (68.4% of total FAs, PUFAs (5.3%, MUFAs (21.3%, t-FAs (3.6% and CLA (0.8%. The most represented fatty acids were: 16:0 (24.5%, 9cis-18:1 (18.2%, 18:0 (9.6%, 14:0 (9.5%, 10:0 (9.3% and 12:0 (4.5%. Nutritional indices were 2.8-6.8 for n-6/n-3 ratio; 2.3-2.9 for AI; and 2.7-3.2 for TI. Milk produced by small scale plants, with no milk fat standardisation, showed greater differences in fatty acid profile as compared to industrial plants milk. Large scale production is characterised by commingled bulk tank milk of different origins and then is more homogeneous. The whole goat milk supply chain should be controlled to obtain milk with fatty acids of high nutritional value.

  18. Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.

    Science.gov (United States)

    Souza, Viviane; Nader Filho, Antonio; de Castro Melo, Poliana; Ferraudo, Guilherme Moraes; Antônio Sérgio, Ferraudo; de Oliveira Conde, Sandra; Fogaça Junior, Flavio Augusto

    2012-10-01

    The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines' insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents' transmission mechanism within the context of the farms investigated. PMID:24031997

  19. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk

    OpenAIRE

    Geser Nadine; Stephan Roger; Hächler Herbert

    2012-01-01

    Abstract Background The impact of food animals as a possible reservoir for extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae, and the dissemination of such strains into the food production chain need to be assessed. In this study 334 fecal samples from pigs, cattle, chicken and sheep were investigated at slaughter. Additionally, 100 raw milk samples, representing bulk tank milk of 100 different dairy farms, 104 minced meat (pork and beef) samples and 67 E. coli isolates fro...

  20. Biosensor assay for determination of haptoglobin in bovine milk.

    Science.gov (United States)

    Akerstedt, Maria; Björck, Lennart; Persson Waller, Karin; Sternesjö, Ase

    2006-08-01

    Despite more than 30 years of research into mastitis diagnostics, there are few alternatives to the somatic cell count (SCC) in practical use for identification of cows with subclinical mastitis. Mastitis is not only an animal welfare problem, but also affects the yield, composition and technological properties of milk. Hence, dairy cooperatives give farmers a premium quality payment to encourage low SCC although there is no clear scientific data defining the level of SCC in bulk tank milk that is associated with additional benefits in terms of milk quality. Recent research on alternative markers for inflammatory reactions in the lactating cow, e.g. in mastitis, includes investigations of the acute phase protein, haptoglobin (Hp). So far, the content of Hp in milk has mainly been studied in relation to mastitis diagnostics, with little attention given to its importance for milk composition and technological properties. At present, Hp in milk is measured using ELISA, but this technique is not suitable for routine large-scale analysis. In recent years, optical biosensor technology has been used for automated and rapid quantitative analysis of different components in milk, but so far not for analysis of acute phase proteins. The aim of the present study was to develop a rapid and sensitive biosensor method to determine Hp in milk. An affinity sensor assay based on the interaction between Hp and haemoglobin was developed using surface plasmon resonance (SPR) biosensor technology. The assay was used to analyse Hp in composite milk samples from cows without any clinical signs of mastitis and quarter milk samples with a weak to strong reaction in the California Mastitis Test (CMT). A commercial ELISA for determination of Hp in milk was used for comparison. The limit of detection (LOD) of the biosensor assay was determined as 1.1 mg/l. Within-assay and between-day variations were determined both with bulk tank milk spiked with human Hp and with composite milk samples

  1. Oxidative stability of structured lipids containing C18:0, C18:1, C18:2, C18:3 or CLA in sn 2-position - as bulk lipids and in milk drinks

    DEFF Research Database (Denmark)

    Timm Heinrich, Maike; Nielsen, Nina Skall; Xu, Xuebing;

    2004-01-01

    In this study, we compared the oxidative stability of a specific structured lipid (SL) containing conjugated linoleic acid (CLA) in the sn2-position with SL containing other C18 fatty acids of different degree of unsaturation (stearic, oleic, linoleic or linolenic acid). SL was produced by enzyma......In this study, we compared the oxidative stability of a specific structured lipid (SL) containing conjugated linoleic acid (CLA) in the sn2-position with SL containing other C18 fatty acids of different degree of unsaturation (stearic, oleic, linoleic or linolenic acid). SL was produced...... by enzymatic interesterification with caprylic acid. Oxidative stability was compared in the five lipids themselves and in milk drinks containing 5% of the different SL. During storage, samples were taken for chemical and physical analyses. Moreover, sensory assessments were performed on milk drinks....... The oxidative stability of our SL was very different when comparing (a) bulk lipids and milk drink and (b) the five different batches of each product. SL based on oleic acid was the most unstable as bulk lipid, while SL based on linoleic acid was the most unstable in milk drink. SL based on CLA was the second...

  2. THE MICROBIOLOGICAL LOAD OF SHEEP MILK FROM PRIMARY PRODUCTION TO ITS PROCESSING

    Directory of Open Access Journals (Sweden)

    Zuzana Farkašová

    2010-05-01

    Full Text Available In the breeding with the average number of 220 sheep (zošľachtená valaška with traditional hand milking in the Eastern Slovakia the microbiological load of milk during the process of primary production, transport, before and after pasteurisation as well as during dairy processing to cheese curd was observed. The results in three seasons were compared to those obtained at finishing of milking in the season before. The microbiological load of milk was observed using the bacteriological methods for determination of the presence of Staphylococcus sp. and other bacteria, and determination of the total number of staphylococci: a  in milliliter of pool milk sample; b  the transport control – smears from transport tank and determination of the total number of staphylococci in the tank milk sample; c bacteriological examination of bulk tank milk in the dairy plant before and after pasteurisation, including examination of cheese curd. After pasteurisation no staphylococci were recorded as in milk as in cheese. Out of 112 strains of Staphylococcus aureus only four strain produced staphylococcal enterotoxins (SE, but in another 7 strains a gene for production of SE, type C was found. The measures introduced during the following season led to the fact that total numbers of coagulase-positive staphylococci in milk within the process of primary production and transport did not exceed the limit permitted by legislation, and after pasteurisation of milk and cheese curd they were not found at all.  doi:10.5219/58

  3. Impact of three inactivated bovine viral diarrhoea virus vaccines on bulk milk p80 (NS3) ELISA test results in dairy herds.

    Science.gov (United States)

    Sayers, Ríona G; Sayers, Gearóid P; Graham, David A; Arkins, Sean

    2015-07-01

    Bovine viral diarrhoea virus (BVDV) is endemic in many countries and vaccines are used as a component of control and eradication strategies. Surveillance programmes to detect exposure to BVDV often incorporate the use of bulk milk (BM) testing for antibodies against BVDV p80 (NS3), but vaccination can interfere with these results. The aim of this study was to evaluate whether BVDV vaccines would confound BM testing for specific antibodies in a nationally representative group of commercial dairy farms in the Republic of Ireland. A total of 256 commercial dairy herds were included in the statistical analysis. Quarterly BM or serum samples from selected weanling heifers (unvaccinated homeborn youngstock) were assessed by ELISA for antibodies against the BVDV p80 subunit and whole virus. Wilcoxon rank-sum and receiver operating characteristic (ROC) analyses were used to examine differences among groups vaccinated with one of three commercially available inactivated BVDV vaccines. Two of the three vaccines showed evidence of interference with ELISA testing of BM samples. ROC analysis highlighted that one vaccine did not reduce the discriminatory power of the BVDV p80 ELISA for identification of herds with evidence of recent BVDV circulation, when compared with unvaccinated herds; thus, administration of this vaccine would allow uncomplicated interpretation of BM ELISA test results in vaccinated seropositive herds. Seasonal differences in BM antibody results were identified, suggesting that the latter half of lactation is the most suitable time for sampling dairy herds containing predominantly spring calving cows. The results of the present study are likely to prove useful in countries allowing vaccination during or following BVDV eradication, where BM testing is required as part of the surveillance strategy. PMID:25986132

  4. The Influence of Season on the Cow Milk Quantity, Quality and Hygiene

    Directory of Open Access Journals (Sweden)

    Ludovic Toma Cziszter

    2012-10-01

    Full Text Available The purpose of this study was to evaluate the effects of season of collection on the quantity, quality and hygienicproperties of the raw milk delivered from one dairy farm. The studied traits were: bulk tank milk yield, chemicalcomposition (fat, protein, lactose, and total solids, freezing point, density, total bacteria count, coliform bacteriacount and somatic cell count, during years 2010 and 2011. A total of 727 samples were drawn and analysed in twolaboratories, using the standard methods. Average milk production per day per head in the farm was 13.58 kg,obtained from 252 cows. Year of collection had a significant effect on the bulk tank raw milk yield, quality andhygiene, except for freezing point and total bacteria count. The raw milk yield and chemical composition improved(p<0.05 from year 2010 to year 2011, as well as the hygienic quality. Season of collection had a significant (p<0.05influence on the milk yield and chemical composition, the highest milk yield with the lowest concentration beingobtained during summer, while the lowest milk yield with the highest chemical composition was obtained in winter.Physical properties of the raw milk were less affected by the season of collection, with the lowest freezing point inthe winter and the highest density in the autumn. The highest somatic cell count and coliform bacteria count wasobtained during the spring and the lowest total bacteria count was obtained in winter season. There was a significant(p<0.05 interaction between year and season of production for all raw milk traits.

  5. Patrones temporales de recuento de células somáticas, grasa, proteína y nitrógeno ureico en leche de estanque y su asociación con fertilidad en ganado lechero en la zona centro-sur de Chile Temporal patterns of bulk tank somatic cell count, fat, protein, and urea nitrogen and their association with fertility in Central-Southern Chilean dairy cattle

    Directory of Open Access Journals (Sweden)

    PJ Pinedo

    2010-01-01

    Full Text Available El objetivo de este estudio fue describir y evaluar la dinámica de los recuentos de células somáticas (RCS, el nitrógeno ureico (NUL, el porcentaje de grasa y el porcentaje de proteína de la leche de estanque (LE y analizar su asociación con índices reproductivos, considerando muestras mensuales de leche de estanque de 187 rebaños lecheros (~12.000 vacas lecheras en la zona centro-sur de Chile entre 1997 y 2007. Los índices reproductivos incluidos en el estudio fueron intervalo parto primer servicio (IPPS, d, intervalo parto preñez (IPP, d y servicios por concepción (SC. Los RCS de estanque resultaron positivamente asociados con tamaño de rebaño, producción de leche (ME 305d, sólidos en leche y NUL. El nitrógeno ureico de la leche de estanque resultó positivamente asociado con producción de leche y LE RCS. El promedio del intervalo parto primer servicio fue de 92,5 d y mostró una asociación positiva con los niveles de proteína de estanque y NUL. El promedio del intervalo parto preñez fue 138,9 d y evidenció una asociación positiva con LE NUL. Se concluye que los cambios más significativos en los componentes la leche de estanque durante los últimos 10 años en planteles lecheros de la zona centro sur de Chile correspondieron a una disminución en RCS y a un incremento en NUL. Los cambios en RCS reflejan una mejora en sanidad mamaria, calidad y niveles de producción de leche. Las concentraciones de NUL en leche de estanque fueron elevadas durante los últimos 5 años del periodo en estudio y dependieron de factores tales como producción de leche, porcentaje de proteína, mes y año de muestreo. NUL resultó el componente con la asociación más notoria con los parámetros reproductivos en análisis.The objective of this study was to describe and evaluate the dynamics of bulk tank (LE somatic cell counts (RCS, milk urea nitrogen (NUL, fat (% and protein (% and to analyze their association with reproductive indexes

  6. 7 CFR 58.218 - Surge tanks.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Surge tanks. 58.218 Section 58.218 Agriculture....218 Surge tanks. If surge tanks are used for hot milk, and temperatures of product including foam being held in the surge tank during processing, is not maintained at a minimum of 150 °F, then two...

  7. Milking hygiene: new issues and opportunities from automatic milking

    Directory of Open Access Journals (Sweden)

    Lotte Bach Larsen

    2010-01-01

    Full Text Available Automatic milking offers the opportunity of in-line measurements of milk components, check of milking and cleaning procedures,and surveillance through the management program. These advantages may directly benefit the milk quality.Diversion of abnormal milk at time of milking is critical to the milk quality. It is proposed to define abnormal milk as milkbeing visibly changed in homogeneity or colour from that of normal milk. Several enzymes and other milk componentsmay be involved in the formation of clots in the milk. Based on reported changes in primarily the milk protein fraction asa response to infection of the mammary gland, different explanations for the generation of precipitates in mastitis milkare discussed. Automatic milking systems (AMS should have sensors to monitor and divert abnormal milk. The managementsystem of AMS provides an excellent opportunity to introduce Hazard Analyses Critical Control Points (HACCPsystems for surveying the milk quality. HACCP-based proposals are given for avoiding contamination of the bulk milk withmilk from cows with clinical mastitis and for ensuring a low bulk milk bacterial count.

  8. Numerical Simulation of Bulk Cement Truck Tank Unloading Process Based on Fluent Secondary Development%基于Fluent二次开发的散装水泥运输车卸料过程数值模拟

    Institute of Scientific and Technical Information of China (English)

    纪宏超; 李耀刚; 王玉彬

    2012-01-01

    Aiming at the situation that fluid software Fluent cannot be taken fall use of in the design of bulk cement truck tank in domestic, the programming language of VB 6,0 was used to develop man-machine interface for secondary development of Fluent software. The special features of numerical simulation on bulk cement truck tank unloading process were achieved. The effects of air distributor on unloading process in bulk cement truck tank unloading process were simulated by an example. The results indicate that the interface is intuitive and the operation is simple. The fluidized and unloading process of bulk cement truck tank can be well simulated and applied to the development of cement truck tank. Enlarging the diameter of air distributor can reduce the unloading time effectively. The unloading efficiency and the stability of unloading process can be improved. Under the permitted conditions, the diameter of air distributor should be enlarged.%针对国内散装水泥车罐体设计中不能充分利用流体软件Fluent 的情况,利用VB 6.0编程语言编程开发人机交互界面,时Fluent软件进行二次开发,实现散装水泥车罐体卸料过程的数值模拟的专用功能;通过一个实例模拟散装水泥罐车在卸料过程中布风板对卸料过程的影响.结果表明,把Gambit和Fluent内部封装,界面直观、操作简单,能够很好地模拟水泥罐车流态化与卸料过程,并应用到水泥车罐体的开发中;增大布风板的直径能够有效地减少卸料时间,提高卸料效率,并且提高卸料过程的平稳性,在条件允许的情况下应增大布风板的直径.

  9. Short communication: Microbial quality of raw milk following commercial long-distance hauling.

    Science.gov (United States)

    Darchuk, Emily M; Meunier-Goddik, Lisbeth; Waite-Cusic, Joy

    2015-12-01

    Hauling is a critical part of the commercial milk supply chain, yet very few studies have aimed to understand its effect on raw milk quality. This study focused on the effect of extended-duration tanker use during hauling on raw milk quality at a commercial facility. Standard tanker use [cleaned-in-place (CIP) once per 24h] served as a control and an incremental between-load water rinse with sanitizer treatment (RS) was evaluated to mitigate any effect from extended duration hauling. During this study, 1 commercial truck with 2 trailers was monitored for 10d. The truck collected milk at a large dairy farm, transported the milk to a manufacturing facility, and then returned to the same farm for a second load. Each round-trip journey took between 10 and 12h, allowing for 2 loads per 24-h use period. Following the second delivery, the truck was cleaned by CIP treatment starting a new treatment day. Producer samples were collected from the raw milk bulk tank on the farm before loading milk into the tanker. The same milk was sampled directly out of the tanker truck before unloading at the manufacturer. Effect on individual bacteria count, thermophilic spore count, and preliminary incubation count was quantified through common industry tests. Surface sponge swabs were also used to monitor tanker sanitation and the efficacy of cleaning treatments. Results did not identify a negative effect on raw milk quality due to extended duration hauling. Whereas the addition of RS did not provide any measurable quality benefits for the microbial milk quality, swab results demonstrated that the RS treatment was able to reduce surface bacteria in the tanker, although not to the same level as the full CIP treatment. Based on this study, current CIP practices for long distance milk hauling appear to be effective in mitigating any measurable effect on raw milk quality. PMID:26506549

  10. A space-time analysis of Mycoplasma bovis: bulk tank milk antibody screening results from all Danish dairy herds in 2013-2014

    DEFF Research Database (Denmark)

    Arede, Margarida; Nielsen, Per Kantsø; Ahmed, Syed Sayeem Uddin;

    2016-01-01

    Mycoplasma bovis is an important pathogen causing severe disease outbreaks in cattle farms. Since 2011, there has been an apparent increase in M. bovis outbreaks among Danish dairy cattle herds. The dairy cattle industry performed cross-sectional antibody screening for M. bovis on four occasions...

  11. Staphylococcus aureus isolates from goat and sheep milk seem to be closely related and differ from isolates detected among bovine milk

    Directory of Open Access Journals (Sweden)

    Axel eMerz

    2016-03-01

    Full Text Available Dairy goat and sheep farms suffer severe economic losses due to intramammary infections, with S. aureus representing the main cause of clinical mastitis in small ruminants. In addition, S. aureus contamination of goat and sheep milk may cause staphylococcal food poisoning, as many traditional caprine and ovine milk products are not subjected to pasteurization. Data on virulence and antimicrobial resistance genes, as well as on the clonality of S. aureus detected in goat and sheep milk is scarce. Therefore, it was the aim of this study to determine i spa types and clonal complexes and ii virulence and resistance gene profiles of S. aureus isolated from goat and sheep milk. A total of 162 milk samples from sheep and goats presenting signs of an intramammary infection and 104 bulk milk samples were collected. While low prevalence rates of S. aureus were detected on single animal level, 46% of the bulk tank milk samples from small ruminants were positive for S. aureus. All isolates were spa typed and clonal complexes and virulence and resistance gene patterns were determined using a DNA microarray. Data from 49 S. aureus isolates was included in the statistical analysis and the construction of a SplitsTree. The analyzed isolates could be assigned to eleven clonal complexes, with the large majority of goat and sheep isolates being assigned to CC130 and CC133. The findings of this study suggest that S. aureus shows pronounced adaptation to small ruminants in general, but not to sheep or goats in particular. Although some common characteristics among S. aureus from caprine, ovine, and bovine milk samples were observed, S. aureus from small ruminants seem to form a distinct population. As 67% of the detected S. aureus strains exhibited at least one enterotoxin gene, many caprine or ovine raw milk products may be contaminated with low levels of enterotoxigenic S. aureus, stressing the importance of strict maintenance of the cold chain.

  12. Staphylococcus aureus Isolates from Goat and Sheep Milk Seem to Be Closely Related and Differ from Isolates Detected from Bovine Milk.

    Science.gov (United States)

    Merz, Axel; Stephan, Roger; Johler, Sophia

    2016-01-01

    Dairy goat and sheep farms suffer severe economic losses due to intramammary infections, with Staphylococcus aureus representing the main cause of clinical mastitis in small ruminants. In addition, S. aureus contamination of goat and sheep milk may cause staphylococcal food poisoning, as many traditional caprine and ovine milk products are not subjected to pasteurization. Data on virulence and antimicrobial resistance genes, as well as on the clonality of S. aureus detected in goat and sheep milk is scarce. Therefore, it was the aim of this study to determine (i) spa types and clonal complexes (CC) and (ii) virulence and resistance gene profiles of S. aureus isolated from goat and sheep milk. A total of 162 milk samples from sheep and goats presenting signs of an intramammary infection and 104 bulk milk samples were collected. While low prevalence rates of S. aureus was detected on single animal level, 46% of the bulk tank milk samples from small ruminants were positive for S. aureus. All isolates were spa typed and CC and virulence and resistance gene patterns were determined using a DNA microarray. Data from 49 S. aureus isolates was included in the statistical analysis and the construction of a SplitsTree. The analyzed isolates could be assigned to eleven CC, with the large majority of goat and sheep isolates being assigned to CC130 and CC133. The findings of this study suggest that S. aureus shows pronounced adaptation to small ruminants in general, but not to sheep or goats in particular. Although some common characteristics among S. aureus from caprine, ovine, and bovine milk samples were observed, S. aureus from small ruminants seem to form a distinct population. As 67% of the detected S. aureus strains exhibited at least one enterotoxin gene, many caprine, or ovine raw milk products may be contaminated with low levels of enterotoxigenic S. aureus, stressing the importance of strict maintenance of the cold chain. PMID:27014240

  13. Occurrence and survival of verocytotoxin-producing Escherichia coli O157 in raw cow's milk in The Netherlands.

    Science.gov (United States)

    Heuvelink, A E; Bleumink, B; van den Biggelaar, F L; Te Giffel, M C; Beumer, R R; de Boer, E

    1998-12-01

    From May through November 1997, 1,011 samples of raw milk from bulk storage tanks were examined for the presence of verocytotoxin-producing Escherichia coli of serogroup O157 (O157 VTEC) by immunomagnetic separation following selective enrichment. The samples originated from 1,011 different dairy herds located throughout the Netherlands. O157 VTEC was not isolated from any of the milk samples examined. Additionally, survival of O157 VTEC in raw and UHT-sterilized cow's milk at 7 and 15 degrees C was studied, both in the absence and presence of an activated lactoperoxidase-thiocyanate-hydrogen peroxide system (LPS). Results indicated that the O157 VTEC strain tested was able to grow in raw milk at 7 degrees C as well as at 15 degrees C. Naturally occurring amounts of thiocyanate and hydrogen peroxide in the raw milk tested were not sufficient to activate the LPS. Although the LPS exhibited an antimicrobial activity against O157 VTEC in LPS-activated sterilized milk, O157 VTEC populations were not (or not as obviously) reduced in LPS-activated raw milk. Possibly background microflora were more sensitive to the LPS than the O157 VTEC test strain. It was concluded that raw milk contaminated with O157 VTEC will remain a hazard if kept at 7 or 15 degrees C. Effective pasteurization and avoiding postpasteurization contamination are necessary to ensure the safety of milk. PMID:9874335

  14. 46 CFR 154.446 - Tank design.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Tank design. 154.446 Section 154.446 Shipping COAST... SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Independent Tank Type B § 154.446 Tank design. An independent tank type B must meet the calculations under §...

  15. The bacteriological quality of goat and ovine milk

    Directory of Open Access Journals (Sweden)

    Kateřina Bogdanovičová

    2015-05-01

    Full Text Available This study concentrates on information concerning the microbiological hazards that can be present in raw milk from animal species other than cows. A total of 54 (23 of ovine and 31 of goat bulk tank milk samples from 10 farms in the Czech Republic were collected in years 2013 - 2014. The sampling was done at regular time intervals during the whole year, with five to eight samples collected from each of the 10 dairy farms involved in the study. All milk samples were collected into sterile sampling bottles and transported in a cooler sampling case to the laboratory for immediate examination. Farms were randomly selected to cover the whole area of the Czech Republic. The prevalence and characteristic of Escherichia coli, Staphylococcus aureus, Salmonella spp., Campylobacter spp. and Listeria monocytogenes was studied. Raw cow's milk can be contaminated by E. coli intramammarily during clinical or subclinical mastitis and either directly through animal feces or indirectly during milk collection through farm employees or the milking equipment. E. coli was detected in 90.3% of the goat milk and 95.7% of the ovine milk samples. The genes encoding Shiga toxins 1 and 2- (stx1, stx2 were not detected and no STEC was identified. The Eae was the detected in 3 (4.6% isolates. S. aureus was detected in 9 (29.0% samples of goat milk and 8 (34.8% samples of ovine milk. A total 12 (57.1% enterotoxin positive S. aureus were obtained; 6 (28.6% were positive for the production of sec encoding enterotoxin SEC; in 4 (19.0% isolates the gene seh was detected; 2 (9.5% isolates were proven positive for seg (4.8% and combination seg and sei (4.8%. The presence of MRSA was not detected in the tested samples in our study. L. monocytogenes was detected in 1 (3.2% samples of goat milk and 1 (4.3% samples of ovine milk. The serotype (1/2a, 1/2b was detected in our study. Campylobacter spp. and Salmonella spp. were not isolated from any of the samples. These results form

  16. 7 CFR 58.250 - Dry whole milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Dry whole milk. 58.250 Section 58.250 Agriculture... Products Bearing Usda Official Identification § 58.250 Dry whole milk. Dry whole milk in commercial bulk... Grades of Dry Whole Milk. Quality requirements for dry whole milk in consumer packages shall be for...

  17. Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms

    Directory of Open Access Journals (Sweden)

    Viviane Souza

    2012-12-01

    Full Text Available The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE. Samples' collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%, CMT-reagent cow milk (21.7%, mechanical milking machines' insufflators (21,7%, milk in milk pails (6.6% and the milk in community bulk tanks (5.6%. Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%, followed by milk samples from CMT-reagent dairy cows (5.8% and mechanical milking machine insufflators (3.8%. The above shows the relevance of these sites in the agents' transmission mechanism within the context of the farms investigated.

  18. ANALYSIS OF MILK QUALITY AND ITS IMPORTANCE FOR MILK PROCESSORS

    Directory of Open Access Journals (Sweden)

    AGATHA POPESCU

    2013-07-01

    Full Text Available The paper aimed to present some aspects regarding milk quality and its importance for milk processors , taking into account a study case at FLAV O’RICH DAIRY INC,USA. The study analyses how milk quality is checked from the bulk milk to final product according to the Milk Quality Program in force. The main aspects concerning raw milk selection criteria such as : antibiotic test, temperature, bacteria, organoleptic properties , acidity, somatic cell count , but also main milk components such as water, butterfat , total solids, protein, lactose , solids non fats , minerals, acids have been approached . Also a comparison for 7 butterfat producers for East Fluid Group has been done . Milk processing assures the destruction of human pathogens , the maintenance of product quality without significant loss of flavor, appearance, physical and nutritive properties and the selection of organisms which may produce unsatisfactory products.

  19. Interspecies and seasonal differences of retinol in dairy ruminant´s milk

    Directory of Open Access Journals (Sweden)

    Lucia Hodulová

    2015-08-01

    Full Text Available Milk is an essential source of macronutrients and among lipophilic vitamins is significant source of retinol. The contribution of milk to the reference daily intake for retinol varies from 11% to 16%, worldwide. The most consumed dairy products are fresh, dehydrated and condensed milk in which the amonuts of retinol are not modified to those of in whole milk. Retinol is essential to ensure a good functionality of the immune system and plays a critical role in vision, reproduction, cell differentiation as well as growth and development and is found only in animal tissues. The aim of our study was to evaluate the interspecies differences in the retinol concentration of whole raw bovine, caprine and ovine milk and to observe seasonal variation of retinol in bulk tank milk samples. Samples of raw milk were colleceted on different farms in the Czech Republic between 2013 and 2014. Retinol was measured by ultra high performance liquid chromatography with UV detection (325 nm in isocratic mode after alkaline saponification with methanolic potassium hydroxide solution and liquid-liquid extraction into non polar organic solvent of whole raw milk. To avoid vitamin losses or degradation during the procedure, antioxidants were added to the sample extraction media. Our results indicate significant interspecies differences between bovine and ovine milk and caprine and ovine milk. Concentration of retinol is very similar in bovine and caprine milk 0.96 ±0.11 mg/L, 0.94 ±0.25 mg/L, respectively. The mean concentration in sheep´s milk is 1.75 ±0.24 mg/L. The seasonal variation of retinol in raw bovine milk was detected as high significant, with the highest concentration during winter. These results contribute to the nutrition evaluation of milk in the Czech Republic and indicate, that the sheep´s milk is the best source of retinol among the milks of ruminants kept in the Czech Republic, however it is not used in its fluid form for human consumption.

  20. TANK CAR CONSTRUCTION REFINMENT

    Directory of Open Access Journals (Sweden)

    A. N. Soberzhansjkyj

    2010-06-01

    Full Text Available The increase of volume and load-carrying capacity of tank cars is an urgent task for improving the efficiency of transportation of liquid bulk cargoes. Variants of the constructive and technical approaches, which allow increasing the specified indices, are considered. After the analysis the most rational constructive scheme meeting the modern requirements for tank cars and allowing to raise their productivity is chosen.

  1. 33 CFR 157.147 - Similar tank design: Inspections on foreign tank vessels.

    Science.gov (United States)

    2010-07-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels...

  2. Application of One-step RT-PCR Used to Detect Bulk Milk in BVDV Eradication Programme%一步法RT—PCR检测大缸奶在BVDV清除计划中的应用

    Institute of Scientific and Technical Information of China (English)

    黄凯; 邵晓磊; 李锡智

    2011-01-01

    本文利用一步法RT—PCR对28个牛场共计50份大缸奶样进行了BVDV核酸检测。在与全群抗原检测结果对比后发现.9个已知存有PI牛的泌乳牛群,其大缸奶核酸检测均为阳性,其余41个已知无PI牛的泌乳牛群.除1个因为存在急性感染牛造成阳性结果外,其余40个均为阴性。由此可见,本方法对与泌乳牛群内是否存在PI牛可以做出准确判断,其检测灵敏度和特异性均为100%,阳性预测结果可信度为0.9(9/10),阴性预测结果可信度为0.98(40/41)。此外.针对50个大缸奶样进行的抗体检测表明,对于已感染牛场,泌乳牛群是否存在PI牛,抗体水平没有明显差异(OD1.12±0.12vsOD1.34±0.23,P〉0.05)。实验室条件下,本试验使用的RT—PCR方法对于阳性乳的最低检出限为50uL/头,因此理论上,最多可从1000份样品中检出阳性乳成分(总体积为50mL)。综上可知,本试验确立的RT—PCR方法灵敏度高、特异性强,可对泌乳牛群是否存在PI牛进行准确预测,相比大缸奶抗体检测更具实际指导意义,联合ELISA—Ag使用时还可大幅度降低泌乳牛群BVDV清除计划的检测成本,因而值得推广使用。%A single step, single-tube reverse transcriptase-polymerase chain reaction (RT-PCR) test was developed to detect the presence of bovine viral diarrhoea virus(BVDV) in somatic cells from bulk milk samples. The test was validated by examining 50 bulk milk samples from 28 herds with result of BVDV elimination programme. Results showed that a total of 9 groups which contained one persistently infected animal were positive on bulk milk samples. Another 41 herds were negative except one herd which was positive because there was one acute infection cow. So the test was proved to be highly specific and sensitivity, which the positive predictive value(PPV) and the negative predictive value

  3. Colostrum and milk pasteurization improve health status and decrease mortality in neonatal calves receiving appropriate colostrum ingestion.

    Science.gov (United States)

    Armengol, Ramon; Fraile, Lorenzo

    2016-06-01

    The objective of the study was to evaluate if on-farm heat treatment of colostrum and bulk tank milk can improve calf health status and morbidity and mortality rates during the first 21d of life in neonatal Holstein calves receiving appropriate colostrum ingestion. A total of 587 calves were randomly assigned to 2 groups of males and females over 18mo. The nonpasteurized group (n=287, 143 males and 144 females) was fed frozen (-20°C) colostrum (6-8L during the first 12h of life) that was previously reheated up to 40°C. They were also fed refrigerated (4°C) raw milk from the bulk tank that was also reheated up to 40°C (1.8L every 12h). The pasteurized group (n=300, 150 males and 150 females) was also fed colostrum and milk, but both were pasteurized before freezing. Blood samples were drawn from all calves to obtain serum at 2 to 5d of life. Serum total protein (g/dL) was determined using a commercially available refractometer. Colostrum and milk underwent routine bacteriological analysis to determine total plate counts (cfu/mL) and total coliform counts (cfu/mL). All the calves underwent clinical examination every 24h during the first 21d of life. Every day, calves were clinically diagnosed either as being healthy or suffering from respiratory disease, neonatal calf diarrhea, or suffering other diseases. On-farm heat treatment for colostrum and milk reduced total plate counts and total coliform counts between 1 and 2 log10. Pasteurization of colostrum and milk significantly decreased the morbidity and mortality (5.2 and 2.8%) in comparison with calves receiving nonpasteurized colostrum and milk (15.0 and 6.5%), respectively, during the first 21d of life, even in animals receiving appropriate colostrum ingestion. PMID:26995131

  4. Do farm audits improve milk quality?

    NARCIS (Netherlands)

    Flores-Miyamoto, A.; Reij, M.W.; Velthuis, A.G.J.

    2014-01-01

    Milk quality is assessed using bulk milk analysis and by farm audits in the Netherlands. However, the extent of the effect that dairy farm audits have on milk quality is unknown. Data from over 13,000 audits performed on 12,855 dairy farms from February 2006 to April 2008 were merged with laboratory

  5. RESEARCH REGARDING THE CHEMICAL COMPOSITION OF POWDER MILK WITH NUTRIENTS

    OpenAIRE

    Liviu Giurgiulescu; Alina Veronica Lapusan

    2009-01-01

    Powdered milk is a manufactured dairy product made by evaporating milk to dryness. This product has incomposition powder apple, powder carrots, rice flour and corn flour, vitamins, minerals.One purpose of drying milk is to preserve it; milk powder has a far longer self life than liquid milk and does notneed to be refrigerated, due to its low moisture content. Another purpose is to reduce its bulk for economy oftransportation. Milk powders contain all twenty standards amino acids and are high ...

  6. Detection and Enumeration of Streptococcus agalactiae from Bovine Milk Samples by Real-Time Polymerase Chain Reaction.

    Science.gov (United States)

    de Carvalho, Nara Ladeira; Gonçalves, Juliano Leonel; Botaro, Bruno Garcia; Silva, Luis Felipe de Prada E; dos Santos, Marcos Veiga

    2015-09-01

    The aim of this study was to evaluate the use of real-time polymerase chain reaction (qPCR) combined with DNA extraction directly from composite milk and bulk tank samples for detection and enumeration of Streptococcus agalactiae (SAG) causing subclinical mastitis. Dilutions of sterile reconstituted skim milk inoculated with SAG ATCC 13813 were used to establish a standard curve (cfu/mL) for the qPCR assay targeting SAG. The analytical sensitivity and repeatability of the qPCR assay were determined. Bulk tank (BTM; n = 38) and composite milk samples (CM; n = 26) collected from lactating cows with positive isolation of SAG were submitted to the qPCR protocol and SAG plate counting, with results from both methods compared. Amplification of DNA was not possible in two out of 64 samples, indicating that qPCR was able to detect SAG in 96 and 97% of BTM and CM samples, respectively. The inter-assay coefficient of variation was <5%, showing that the technique had adequate repeatability. The qPCR protocol can be a high-throughput and rapid diagnostic assay to accurately detect SAG from BTM and CM samples compared with conventional microbiological culture method. However, the evaluated qPCR protocol is not accurate for enumerating SAG in milk samples, probably due to quantification of DNA of non-viable cells. PMID:26134534

  7. 27 CFR 19.588 - Construction of bulk conveyances.

    Science.gov (United States)

    2010-04-01

    ... compartment) shall be so arranged that it can be completely drained. (3) Each tank car or tank truck shall... device, for carrying required marks or brands shall be provided on each bulk conveyance. (6)...

  8. Potentials to differentiate milk composition by different feeding strategies

    DEFF Research Database (Denmark)

    Slots, Tina; Butler, G.; Leifert, C.;

    2009-01-01

    To investigate the effect of the dietary intake of the cow on milk composition, bulk-tank milk was collected on 5 occasions from conventional (n = 15) and organic (n = 10) farms in Denmark and on 4 occasions from low-input nonorganic farms in the United Kingdom, along with management and production...... (27.5 +/- 0.3 mg/kg of fatty acids), and a high ratio between LA and alpha-linolenic acid (4.7 +/- 0.2). Comparing these 2 production systems with a very extensive nonorganic milk production system relying on pasture as almost the sole feed (95 +/- 4% dry matter intake), it was found...... that the concentrations of conjugated LA (cis-9,trans-11; 17.5 +/- 0.7 g/kg of fatty acids), trans-11-vaccenic acid (37 +/- 2 g/kg of fatty acids), and monounsaturated fatty acids (30.4 +/- 0.6 g/kg of fatty acids) were higher in the extensively produced milk together with the concentration of antioxidants; total alpha...

  9. 19 CFR 151.44 - Storage tanks.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Storage tanks. 151.44 Section 151.44 Customs... Storage tanks. (a) Plans and gauge tables. When petroleum or petroleum products subject to duty at a specific rate per barrel are imported in bulk in tank vessels and are to be transferred into shore...

  10. Microstructure of milk

    Science.gov (United States)

    The fat and protein in milk may be examined by scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy, and any bacteria present may be viewed by light microscopy. The fat exists as globules, the bulk of the protein is in the form of casein micelles, a...

  11. 33 CFR 157.132 - Cargo tanks: Hydrocarbon vapor emissions.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.132 Cargo tanks: Hydrocarbon vapor emissions. Each tank vessel having a COW system under §...

  12. 33 CFR 157.140 - Tank vessel inspections.

    Science.gov (United States)

    2010-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections § 157.140 Tank vessel inspections. (a) Before... having a COW system under § 157.10(e), § 157.10(a)(2), or § 157.10c(b)(2) and each foreign tank...

  13. 46 CFR 153.251 - Independent cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Independent cargo tanks. 153.251 Section 153.251... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Tanks § 153.251 Independent cargo tanks. All independent cargo tank must meet § 38.05-10 (a)(1), (b), (d),...

  14. 33 CFR 157.144 - Tank vessels of the same class: Inspections.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections § 157.144 Tank vessels...

  15. 33 CFR 157.134 - Cargo tank drainage.

    Science.gov (United States)

    2010-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.134 Cargo...

  16. Tank design

    International Nuclear Information System (INIS)

    This paper reports that aboveground tanks can be designed with innovative changes to complement the environment. Tanks can be constructed to eliminate the vapor and odor emanating from their contents. Aboveground tanks are sometimes considered eyesores, and in some areas the landscaping has to be improved before they are tolerated. A more universal concern, however, is the vapor or odor that emanates from the tanks as a result of the materials being sorted. The assertive posture some segments of the public now take may eventually force legislatures to classify certain vapors as hazardous pollutants or simply health risks. In any case, responsibility will be leveled at the corporation and subsequent remedy could increase cost beyond preventive measures. The new approach to design and construction of aboveground tanks will forestall any panic which might be induced or perceived by environmentalists. Recently, actions by local authorities and complaining residents were sufficient to cause a corporation to curtail odorous emissions through a change in tank design. The tank design change eliminated the odor from fuel oil vapor thus removing the threat to the environment that the residents perceived. The design includes reinforcement to the tank structure and the addition of an adsorption section. This section allows the tanks to function without any limitation and their contents do not foul the environment. The vapor and odor control was completed successfully on 6,000,000 gallon capacity tanks

  17. 46 CFR 151.13-5 - Cargo segregation-tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo segregation-tanks. 151.13-5 Section 151.13-5... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... symbols used in lines 1 and 2, in order, under the tank segregation column of Table 151.05....

  18. 46 CFR 154.1844 - Cargo tanks: Filling limits.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tanks: Filling limits. 154.1844 Section 154.1844... STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1844 Cargo tanks... cargo tank is not loaded: (1) More than 98 percent liquid full; or (2) In excess of the...

  19. The Determination of Somatic Cell Count and Some Components of Raw Milk Evaluated By a Private Company in Trakya

    Directory of Open Access Journals (Sweden)

    A. R. Onal

    2007-05-01

    Full Text Available The aim of this study was to determine the amount of bulk tank somatic cell counts. Chemical and microbiological compositions of raw milk produced in Trakya were also reached in order to evaluate the structure of milk production. For this purpose 36 raw milk samples were collected from bulk milk tank within three different location of Trakya (18 samples from Edirne, 10 from Tekirdağ and 8 from Kırklareli. The arithmetic means and standard errors of fat percentages, non-fat dry matter, protein percentages, BTSCC (Bulk Tank Milk Somatic Cell Count and TB (Total Bacteria for Edirne, Tekirdağ and Kırklareli provinces were; 3.70 0.052, 3.60 0.098, 3.76 0.064; 8.34 0.025, 8.50 0.035, 8.39 0.038; 3.05 0.012, 3.09 0.019, 3.05 0.016; 308.555 26.510 SCC/ml (log 5.459 0.04 SCC/ml, 350.200 53.627 SCC/ml (Log 5.500 0.06 SCC/ml, 254.500 37.645 SCC/ml (Log 5.370 0.06 SCC/ml; 479.481 51.777 cfu/ml (Log 5.630 0.05 cfu/ml, 435.716 91.194 cfu/ml (Log 5.5230.12 cfu/ml, 446.958 81.515 cfu/ml (Log 5.602 0.075 cfu/ml respectively. Consequentially, the correlation coefficient for LogBTSCC and fat percentage, non-fat dry matter, protein percentage and LogTB were found to be 0.036, 0.251, 0.421 and 0.219 respectively. A significant (p<0.05 correlation coefficient was obtained between LogBTSCC and protein percentage.

  20. BACILLUS CEREUS: ISOLATION IN JENNET MILK

    Directory of Open Access Journals (Sweden)

    M.L. Scatassa

    2011-01-01

    Full Text Available Jennet milk as human food is hypoallergenic for patients affected by Cow Milk Protein Allergy and multiple food allergies. For these pathologies, jennet milk represents the best alternative to other types of milk. Therefore, jennet milk consumers are very sensible to the effects of pathogens' contaminations, and several hygienic practices during the milk production need to be adopted. During regular monitoring in one Sicilian jennet farm, Bacillus cereus in the milk was detected. In 3 bulk milk samples (maximum concentration: 1.2 x 103 ufc/ml, in 3 individual milk samples (10, 20 e 60 ufc/ml, in the milk filter (5 ufc/cm2, in the soil (maximum concentration: 1.5 x 103 ufc/g, on the hands and the gloves of two milkers, on the animal hide (from 1 to 3 ufc/cm2. No spores were detected. A total of 8 Bacillus cereus s.s. strains were analyzed for diarrhoic toxin, and 6 strains producing enterotoxins resulted. The improvement of environmental and milking hygienic conditions reduced Bacillus cereus concentration.

  1. Bulk undercooling

    Science.gov (United States)

    Kattamis, T. Z.

    1984-01-01

    Bulk undercooling methods and procedures will first be reviewed. Measurement of various parameters which are necessary to understand the solidification mechanism during and after recalescence will be discussed. During recalescence of levitated, glass-encased large droplets (5 to 8 mm diam) high speed temperature sensing devices coupled with a rapid response oscilloscope are now being used at MIT to measure local thermal behavior in hypoeutectic and eutectic binary Ni-Sn alloys. Dendrite tip velocities were measured by various investigators using thermal sensors or high speed cinematography. The confirmation of the validity of solidification models of bulk-undercooled melts is made difficult by the fineness of the final microstructure, the ultra-rapid evolution of the solidifying system which makes measurements very awkward, and the continuous modification of the microstructure which formed during recalescence because of precipitation, remelting and rapid coarsening.

  2. Nitrogen tank

    CERN Multimedia

    2006-01-01

    Wanted The technical file about the pressure vessel RP-270 It concerns the Nitrogen tank, 60m3, 22 bars, built in 1979, and installed at Point-2 for the former L3 experiment. If you are in possession of this file, or have any files about an equivalent tank (probably between registered No. RP-260 and -272), please contact Marc Tavlet, the ALICE Glimos.

  3. 33 CFR 157.146 - Similar tank design: Inspections on U.S. tank vessels.

    Science.gov (United States)

    2010-07-01

    ... OF HOMELAND SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections... Officer in Charge, Marine Inspection, of the zone in which the COW system is inspected, for only one...

  4. First cloud-based service for analyzing storage tank data

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2010-01-15

    Most commercial storage tanks are unmonitored and require manual processes to verify conditions, remediate issues or request servicing. New Boundary Technologies has developed an off-the-shelf solution that eliminates several manual processes. Its TankVista Internet service was launched as the first cloud-based service for continuously monitoring and analyzing the conditions and storage levels of commercial storage tanks, bins, silos and other containers. TankVista takes data from storage tank sensors and translates it into graphics and maps that industry can use to drive new efficiencies in storage tank management. A bulk oil distributor can leverage TankVista to remotely and continuously monitor its own storage tanks as well as those of its clients. TankVista monitors tank level, temperature, pressure, humidity and other storage criteria in order to know exactly when and where to replenish supplies. Rather than re-filling tanks at about 50 per cent capacity, a bulk oil distributor can wait until usage levels dictate more efficient re-filling. The monitoring takes place without manual intervention. TankVista complements the iDigi Tank, which has the unique ability to wirelessly connect dispersed and remote tank assets, and get this information through drop-in wireless mesh technology to the cloud without requiring onsite Internet access. 1 fig.

  5. 33 CFR 157.116 - Required documents: U.S. tank vessels.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.116 Required documents: U.S. tank vessels. The owner, operator, and master of a U.S. tank vessel having a COW system...

  6. 40 CFR 280.43 - Methods of release detection for tanks.

    Science.gov (United States)

    2010-07-01

    ... described in the American Petroleum Institute Publication 1621, “Recommended Practice for Bulk Liquid Stock... paragraph. (b) Manual tank gauging. Manual tank gauging must meet the following requirements: (1) Tank... of release detection. Tanks of 551 to 2,000 gallons may use the method in place of manual...

  7. Milk Allergy

    Science.gov (United States)

    ... lactoferrin, lactulose non-dairy creamers whey, whey hydrolysate Vegan foods are made without animal products, such as eggs or milk. You can buy vegan products at health food stores. Be careful to ...

  8. Correlation between somatic cell count and chemical composition of cooled raw milk in properties of Rio Grande do Norte, Brazil

    Directory of Open Access Journals (Sweden)

    Adriano Henrique do Nascimento Rangel

    2014-06-01

    Full Text Available Due to the damage caused by subclinical mastitis in loss of production and quality of milk, the present study aimed to verify the correlation between somatic cell count (SCC and the chemical composition of cooled raw milk collected in the Agreste region of Rio Grande do Norte, Brazil, in drought and rain seasons. Samples were collected in seven dairy farms during morning time, between January 2010 and March 2012, and sent to the Brazilian et of Milk Quality Laboratory (ESALQ/USP. The contents of protein, fat, lactose, casein, total solids, nonfat dry extract and urea nitrogen, besides of SCC and total bacterial count were performed. Data were submitted to analysis of variance, correlation analysis and comparison of means by Tuckey test , 5%. The average SCC was 604,000 cells/mL and had significant variation in the dry period (558 000 cells/mL and rainy (650 000 cells/mL. The SCC was positively correlated with fat and total solids but negatively with the lactose cow’s milk of bulk tank, regardless of the season in the Agreste of Rio Grande do Norte.

  9. Milk Thistle (PDQ)

    Science.gov (United States)

    ... Professionals Questions to Ask about Your Treatment Research Milk Thistle (PDQ®)–Patient Version Overview Go to Health ... supplement (see Question 9 ). Questions and Answers About Milk Thistle What is milk thistle? Milk thistle is ...

  10. Usage of antimicrobials on 60 dairy farms in Northern Germany and characterization of Methicillin-resistant staphylococcus aureus (MRSA) and extended spectrum beta-lactamases producing Escherichia coli (ESBLs-producing E. coli) isolated from bulk tank milk samples

    OpenAIRE

    Kreausukon, Khwanchai

    2011-01-01

    Das Ziel der Studie war, Informationen über den Einsatz von Antibiotika in deutschen Milchkuhherden zu sammeln. Zudem sollte auf das Vorkommen von MRSA und ESBL-produzierenden E. coli in Tankmilchproben untersucht werden. Fragebögen wurden unter den Herdenmanagern von 60 norddeutschen Betrieben (Herdengröße von 25 bis 3000 Tiere) verteilt, die auf freiwilliger Basis an den Untersuchungen teilnahmen. Tankmilchproben wurden in den Betrieben einmalig entnommen und auf das Vorkommen von MRSA u...

  11. Spoilage potential of Pseudomonas species isolated from goat milk.

    Science.gov (United States)

    Scatamburlo, T M; Yamazi, A K; Cavicchioli, V Q; Pieri, F A; Nero, L A

    2015-02-01

    Pseudomonas spp. are usually associated with spoilage microflora of dairy products due to their proteolytic potential. This is of particular concern for protein-based products, such as goat milk cheeses and fermented milks. Therefore, the goal of the present study was to characterize the proteolytic activity of Pseudomonas spp. isolated from goat milk. Goat milk samples (n=61) were obtained directly from bulk tanks on dairy goat farms (n=12), and subjected to a modified International Organization for Standardization (ISO) protocol to determine the number and proteolytic activity of Pseudomonas spp. Isolates (n=82) were obtained, identified by PCR, and subjected to pulsed-field gel electrophoresis with XbaI macro-restriction. Then, the isolates were subjected to PCR to detect the alkaline protease gene (apr), and phenotypic tests were performed to check proteolytic activity at 7°C, 25°C, and 35°C. Mean Pseudomonas spp. counts ranged from 2.9 to 4.8 log cfu/mL, and proteolytic Pseudomonas spp. counts ranged from 1.9 to 4.6 log cfu/mL. All isolates were confirmed to be Pseudomonas spp., and 41 were identified as Pseudomonas fluorescens, which clustered into 5 groups sharing approximately 82% similarity. Thirty-six isolates (46.9%) were positive for the apr gene; and 57 (69.5%) isolates presented proteolytic activity at 7°C, 82 (100%) at 25°C, and 64 (78%) at 35°C. The isolates were distributed ubiquitously in the goat farms, and no relationship among isolates was observed when the goat farms, presence of apr, pulsotypes, and proteolytic activity were taken into account. We demonstrated proteolytic activity of Pseudomonas spp. present in goat milk by phenotypic and genotypic tests and indicated their spoilage potential at distinct temperatures. Based on these findings and the ubiquity of Pseudomonas spp. in goat farm environments, proper monitoring and control of Pseudomonas spp. during production are critical. PMID:25497792

  12. Tracking sources of microbiologic contamination of raw milk during milking process in dairy farms from Agreste of Pernambuco/ Rastreamento de fontes da contaminação microbiológica do leite cru durante a ordenha em propriedades leiteiras do Agreste Pernambucano

    Directory of Open Access Journals (Sweden)

    Ana Maria Camelo Travassos de Arruda

    Full Text Available The procedures employed in milking process are determinant for obtaining milk with good microbiological quality. The study of contaminant microorganisms permits the identification of its source, what allows the adoption of control measures that will conform to the standards established by legislation. The aim of this work was to determine main points of milk microbiological contamination during milking process. For that mesophilic aerobes (MA, psychrotrophics (PS, coagulase positive Staphylococci (CPE, total coliforms (TC and Escherichia coli (EC were quantified in different steps of milking in six dairy farms and one communitarian bulk tank on Agreste region of Pernambuco, between august and september of 2005. Poor microbiological milk quality was indicated by the high counts of the groups of microorganisms studied. The average counts of microorganisms in milk samples from the dairy farms were 8,3 x 106 (MA, 2,6 x 107 (PS, 1,3 x 104 (CPE, 2,5 x 105 (TC and 3,7 x 103 (EC. In the milk collected from the communitarian bulk tank counts were 6,1 x 107 (MA, 3,4 x 107 (PS, 6,5 x 104 (CPE, > 1,5 x 104 (TC and 2,0 x 103 (EC.Most contaminated points found were residual water of cans, the bottom of cans, bulk tank, teats, first three jets of milk, teatcups, bucket and milker’s hands, and the main cause of milk contamination is improper employ or the absence of good hygiene practices during milking process.Os procedimentos empregados na ordenha são determinantes para a obtenção de leite de boa qualidade microbiológica. O estudo dos grupos de microrganismos contaminantes permite identificar sua origem, o que possibilita a adoção de medidas de controle de forma a atender os padrões estabelecidos pela legislação. O objetivo deste trabalho foi determinar os principais pontos de contaminação microbiológica do leite durante a ordenha. Para isso foram enumerados microrganismos aeróbios mesófilos (AM, psicrotróficos (PS, Estafilococos coagulase

  13. Tank characterization report for double-shell Tank 241-AW-105

    Energy Technology Data Exchange (ETDEWEB)

    DiCenso, A.T.; Amato, L.C.; Franklin, J.D.; Lambie, R.W.; Stephens, R.H. [Los Alamos Technical Associates, Kennewick, WA (United States); Simpson, B.C. [Westinghouse Hanford Co., Richland, WA (United States)

    1994-09-28

    In May 1990, double-shell Tank 241-AW-105 was sampled to determine proper handling of the waste, to address corrosivity and compatibility issues, and to comply with requirements of the Washington Administrative Code. This Tank Characterization Report presents an overview of that tank sampling and analysis effort, and contains observations regarding waste characteristics. It also addresses expected concentration and bulk inventory data for the waste contents based on this latest sampling data and background tank information. This report summarizes the available information regarding the waste in Tank 241-AW-105, and using the historical information to place the analytical data in context, arranges this information in a useful format for making management and technical decisions concerning this waste tank. In addition, conclusions and recommendations are given based on safety issues and further characterization needs.

  14. Milk Money

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    China’s leading food company has expanded its business into the dairy industry A combined financial venture between China’s largest agricultural trading and processing company and a private equity firm formed to milk profits from the dairy business has led to

  15. Feed tank transfer requirements

    Energy Technology Data Exchange (ETDEWEB)

    Freeman-Pollard, J.R.

    1998-09-16

    This document presents a definition of tank turnover. Also, DOE and PC responsibilities; TWRS DST permitting requirements; TWRS Authorization Basis (AB) requirements; TWRS AP Tank Farm operational requirements; unreviewed safety question (USQ) requirements are presented for two cases (i.e., tank modifications occurring before tank turnover and tank modification occurring after tank turnover). Finally, records and reporting requirements, and documentation which will require revision in support of transferring a DST in AP Tank Farm to a privatization contractor are presented.

  16. Feed tank transfer requirements

    International Nuclear Information System (INIS)

    This document presents a definition of tank turnover. Also, DOE and PC responsibilities; TWRS DST permitting requirements; TWRS Authorization Basis (AB) requirements; TWRS AP Tank Farm operational requirements; unreviewed safety question (USQ) requirements are presented for two cases (i.e., tank modifications occurring before tank turnover and tank modification occurring after tank turnover). Finally, records and reporting requirements, and documentation which will require revision in support of transferring a DST in AP Tank Farm to a privatization contractor are presented

  17. Coagulation properties of milk

    OpenAIRE

    Hallén, Elin

    2008-01-01

    Concentrations of the different proteins in milk are important for the outcome of the coagulation processes which yield our dairy products, whereas total milk protein content is a poor indicator of coagulation properties of milk. In order to design the milk protein composition to meet dairy processing requirements, selection for genetic variants of milk proteins have been proposed. This work aimed to study genetic milk protein polymorphism and its association with the detailed milk protein co...

  18. Tank Insulation

    Science.gov (United States)

    1979-01-01

    For NASA's Apollo program, McDonnell Douglas Astronautics Company, Huntington Beach, California, developed and built the S-IVB, uppermost stage of the three-stage Saturn V moonbooster. An important part of the development task was fabrication of a tank to contain liquid hydrogen fuel for the stage's rocket engine. The liquid hydrogen had to be contained at the supercold temperature of 423 degrees below zero Fahrenheit. The tank had to be perfectly insulated to keep engine or solar heat from reaching the fuel; if the hydrogen were permitted to warm up, it would have boiled off, or converted to gaseous form, reducing the amount of fuel available to the engine. McDonnell Douglas' answer was a supereffective insulation called 3D, which consisted of a one-inch thickness of polyurethane foam reinforced in three dimensions with fiberglass threads. Over a 13-year development and construction period, the company built 30 tanks and never experienced a failure. Now, after years of additional development, an advanced version of 3D is finding application as part of a containment system for transporting Liquefied Natural Gas (LNG) by ship.

  19. Microencapsulation of babassu coconut milk

    Directory of Open Access Journals (Sweden)

    Audirene Amorim Santana

    2013-12-01

    Full Text Available The objective of this study was to obtain babassu coconut milk powder microencapsulated by spray drying process using gum Arabic as wall material. Coconut milk was extracted by babassu peeling, grinding (with two parts of water, and vacuum filtration. The milk was pasteurized at 85 ºC for 15 minutes and homogenized to break up the fat globules, rendering the milk a uniform consistency. A central composite rotatable design with a range of independent variables was used: inlet air temperature in the dryer (170-220 ºC and gum Arabic concentration (10-20%, w/w on the responses: moisture content (0.52-2.39%, hygroscopicity (6.98-9.86 g adsorbed water/100g solids, water activity (0.14-0.58, lipid oxidation (0.012-0.064 meq peroxide/kg oil, and process yield (20.33-30.19%. All variables influenced significantly the responses evaluated. Microencapsulation was optimized for maximum process yield and minimal lipid oxidation. The coconut milk powder obtained at optimum conditions was characterized in terms of morphology, particle size distribution, bulk and absolute density, porosity, and wettability.

  20. Protein, casein and micellar salts in milk: Current content and historical perspectives

    NARCIS (Netherlands)

    Bijl, E.; Valenberg, van H.J.F.; Huppertz, T.; Hooijdonk, van A.C.M.

    2013-01-01

    The protein and fat content of Dutch bulk milk has been monitored since the 1950s and has increased considerably, by 11 and 20%, respectively, whereas milk yield has more than doubled. The change in protein and fat content of milk is advantageous for the dairy industry, as these are the 2 most econo

  1. Covariance among milking frequency, milk yield, and milk composition from automatically milked cows

    DEFF Research Database (Denmark)

    Løvendahl, Peter; Chagunda, G G

    2011-01-01

    Automatic milking systems allow cows voluntary access to milking and concentrates within set limits. This leads to large variation in milking intervals, both within and between cows, which further affects yield per milking and composition of milk. This study aimed to describe the degree to which ...

  2. 33 CFR 157.118 - Required documents: Foreign tank vessels.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.118 Required documents... having a COW system under § 157.10c(b)(2) shall ensure that the vessel does not enter the...

  3. Discrepancies in Evaluating Farm Management Routines as Risk Factors of Raw Milk and Udder Hygiene in Selected Dairy Farms of Fars Province, Iran

    Directory of Open Access Journals (Sweden)

    Mehdi MOHEBBI-FANI

    2016-07-01

    Full Text Available The management practices relevant to bulk tank milk quality were studied in 29 dairy farms of Fars Province, Iran. Farm management practices were obtained by completion of a questionnaire and direct observation. Bulk milk was evaluated by performing standard plate count (SPC, preliminary incubation count (PIC, laboratory pasteurization count (LPC, coliform count (CC, somatic cell count (SCC and detection of the contagious mastitis agents. The farms were divided into low and high SPC groups (below and above 100,000 CFU/mL based on Iranian standards. Comparisons of the laboratory results between groups were done using two independent samples t-test. The relationships between the laboratory results were studied by Pearson’s correlation coefficients, all after logarithmic transformation. Associations of managerial risk factors (obtained by the questionnaire and one time of observation with laboratory results were investigated using two independent samples t-test. P-values 0.05. Strong correlations were detected between SPC and PIC, SPC and CC, and PIC and CC, but many of the well explained risk factors of undesired milk quality lacked any relation with high bacterial counts of raw milk. This could be due to the small number of the studied farms, almost similar faults in the farms, wrong answers of the employees to the questions and modification of the milking practices in the presence of an inspector. Infections with Staphylococcus aureus and Mycoplasma bovis could be potential problems in the studied farms, contributing to the elevation of SCC and/or SPC levels. Veterinary interventions could not be based on the questionnaire results. Direct and frequent observations of farm routines could be recommended.

  4. Pregnancy test via milk

    NARCIS (Netherlands)

    Siemes, H.; Woelders, H.

    2011-01-01

    Determining a pregnancy through the milk. Wageningen University is researching the possibilities. The first steps have been taken. Researchers have identified five milk proteins that release a signal of a pregnancy. A pregnancy test via the milk comes within sight.

  5. Analysis of milk sector

    OpenAIRE

    Šemberová, Veronika

    2014-01-01

    Milk and milk products are important sources of protein, vitamins and minerals that are hard to substitute in the human nutrition. In last two decades agricultural underwent several changes and the size of cattle herd decreased. Share of imports on consumption of milk and milk products increased and simultaneously export of raw milk grew. Self-sustainability in milk production so decreased from 118 % to 103 % between 2004 and 2009. The main aim of this thesis called Analysis of the sector ...

  6. AX Tank Farm tank removal study

    Energy Technology Data Exchange (ETDEWEB)

    SKELLY, W.A.

    1999-02-24

    This report examines the feasibility of remediating ancillary equipment associated with the 241-AX Tank Farm at the Hanford Site. Ancillary equipment includes surface structures and equipment, process waste piping, ventilation components, wells, and pits, boxes, sumps, and tanks used to make waste transfers to/from the AX tanks and adjoining tank farms. Two remedial alternatives are considered: (1) excavation and removal of all ancillary equipment items, and (2) in-situ stabilization by grout filling, the 241-AX Tank Farm is being employed as a strawman in engineering studies evaluating clean and landfill closure options for Hanford single-shell tanks. This is one of several reports being prepared for use by the Hanford Tanks Initiative Project to explore potential closure options and to develop retrieval performance evaluation criteria for tank farms.

  7. 33 CFR 157.33 - Water ballast in fuel oil tanks.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Water ballast in fuel oil tanks... OIL IN BULK Vessel Operation § 157.33 Water ballast in fuel oil tanks. A new vessel may not carry ballast water in a fuel oil tank....

  8. 33 CFR 157.160 - Tanks: Ballasting and crude oil washing.

    Science.gov (United States)

    2010-07-01

    ... washing. 157.160 Section 157.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.160 Tanks: Ballasting and crude oil washing. (a) The owner, operator, and master of a tank vessel under §...

  9. 19 CFR 151.28 - Gauging of sirup or molasses discharged into storage tanks.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Gauging of sirup or molasses discharged into... Sugars, Sirups, and Molasses § 151.28 Gauging of sirup or molasses discharged into storage tanks. (a) Plans of storage tank to be filed. When sirup or molasses is imported in bulk in tank vessels and is...

  10. RECENT PROGRESS IN DOE WASTE TANK CLOSURE

    Energy Technology Data Exchange (ETDEWEB)

    Langton, C

    2008-02-01

    The USDOE complex currently has over 330 underground storage tanks that have been used to process and store radioactive waste generated from the production of weapons materials. These tanks contain over 380 million liters of high-level and low-level radioactive waste. The waste consists of radioactively contaminated sludge, supernate, salt cake or calcine. Most of the waste exists at four USDOE locations, the Hanford Site, the Savannah River Site, the Idaho Nuclear Technology and Engineering Center and the West Valley Demonstration Project. A summary of the DOE tank closure activities was first issued in 2001. Since then, regulatory changes have taken place that affect some of the sites and considerable progress has been made in closing tanks. This paper presents an overview of the current regulatory changes and drivers and a summary of the progress in tank closures at the various sites over the intervening six years. A number of areas are addressed including closure strategies, characterization of bulk waste and residual heel material, waste removal technologies for bulk waste, heel residuals and annuli, tank fill materials, closure system modeling and performance assessment programs, lessons learned, and external reviews.

  11. LIFE ESTIMATION OF HIGH LEVEL WASTE TANK STEEL FOR F-TANK FARM CLOSURE PERFORMANCE ASSESSMENT

    Energy Technology Data Exchange (ETDEWEB)

    Subramanian, K

    2007-10-01

    High level radioactive waste (HLW) is stored in underground storage tanks at the Savannah River Site. The SRS is proceeding with closure of the 22 tanks located in F-Area. Closure consists of removing the bulk of the waste, chemical cleaning, heel removal, stabilizing remaining residuals with tailored grout formulations and severing/sealing external penetrations. A performance assessment is being performed in support of closure of the F-Tank Farm. Initially, the carbon steel construction materials of the high level waste tanks will provide a barrier to the leaching of radionuclides into the soil. However, the carbon steel liners will degrade over time, most likely due to corrosion, and no longer provide a barrier. The tank life estimation in support of the performance assessment has been completed. The estimation considered general and localized corrosion mechanisms of the tank steel exposed to the contamination zone, grouted, and soil conditions. The estimation was completed for Type I, Type III, and Type IV tanks in the F-Tank Farm. The tank life estimation in support of the F-Tank Farm closure performance assessment has been completed. The estimation considered general and localized corrosion mechanisms of the tank steel exposed to the contamination zone, grouted, and soil conditions. The estimation was completed for Type I, Type III, and Type IV tanks in the F-Tank Farm. Consumption of the tank steel encased in grouted conditions was determined to occur either due to carbonation of the concrete leading to low pH conditions, or the chloride-induced de-passivation of the steel leading to accelerated corrosion. A deterministic approach was initially followed to estimate the life of the tank liner in grouted conditions or in soil conditions. The results of this life estimation are shown in Table 1 and Table 2 for grouted and soil conditions respectively. The tank life has been estimated under conservative assumptions of diffusion rates. However, the same process of

  12. 49 CFR 172.330 - Tank cars and multi-unit tank car tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Tank cars and multi-unit tank car tanks. 172.330..., TRAINING REQUIREMENTS, AND SECURITY PLANS Marking § 172.330 Tank cars and multi-unit tank car tanks. (a... material— (1) In a tank car unless the following conditions are met: (i) The tank car must be marked...

  13. AX Tank Farm tank removal study

    Energy Technology Data Exchange (ETDEWEB)

    SKELLY, W.A.

    1998-10-14

    This report considers the feasibility of exposing, demolishing, and removing underground storage tanks from the 241-AX Tank Farm at the Hanford Site. For the study, it was assumed that the tanks would each contain 360 ft{sup 3} of residual waste (corresponding to the one percent residual Inventory target cited in the Tri-Party Agreement) at the time of demolition. The 241-AX Tank Farm is being employed as a ''strawman'' in engineering studies evaluating clean and landfill closure options for Hanford single-shell tank farms. The report is one of several reports being prepared for use by the Hanford Tanks Initiative Project to explore potential closure options and to develop retrieval performance evaluation criteria for tank farms.

  14. AX Tank Farm tank removal study

    International Nuclear Information System (INIS)

    This report considers the feasibility of exposing, demolishing, and removing underground storage tanks from the 241-AX Tank Farm at the Hanford Site. For the study, it was assumed that the tanks would each contain 360 ft3 of residual waste (corresponding to the one percent residual Inventory target cited in the Tri-Party Agreement) at the time of demolition. The 241-AX Tank Farm is being employed as a ''strawman'' in engineering studies evaluating clean and landfill closure options for Hanford single-shell tank farms. The report is one of several reports being prepared for use by the Hanford Tanks Initiative Project to explore potential closure options and to develop retrieval performance evaluation criteria for tank farms

  15. Robotic milking and milk quality: effects on bacterial counts, somatic cell counts, freezing point and free fatty acids

    Directory of Open Access Journals (Sweden)

    Yvonne van der Vorst

    2010-01-01

    Full Text Available Changes in milk quality after the introduction of automatic milking systems (AM-systems on dairy farms in TheNetherlands, Germany and Denmark were examined and the data were compared with milk quality results of farms withconventional milking technology. After introduction, a small, but significant increase in total bacterial count, somatic cellcount, freezing point and free fatty acids was observed. The highest levels for total plate count and cell count are foundin the first six months after introduction. After this period the milk quality slightly improves to a more stable level.Risk factors related with milk quality concern general farm characteristics, animal health, AM-system, cleaning and cooling,housing, management skills of the farmer and the hygiene on the farm. Total plate count was significantly relatedto milk yield of the herd, cleaning of the area around the AM-system and the overall hygiene on the farm. Bulk milksomatic cell count appeared to be significantly related to milk yield of the herd and the number of milkings before replacementof the liners. An increased milking frequency is not the only explanation of increased free fatty acid levels. Technicalfactors related to free fatty acids mainly concerned the air inlet in the teat cups, bubbling (excessive air inlet and a toolong post run time of the milk pump. However, several questions regarding the causes of increased free fatty acid levelsremained unclear.

  16. 33 CFR 155.320 - Fuel oil and bulk lubricating oil discharge containment.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Fuel oil and bulk lubricating oil... VESSELS Vessel Equipment § 155.320 Fuel oil and bulk lubricating oil discharge containment. (a) A ship of... area under or around each fuel oil or bulk lubricating oil tank vent, overflow, and fill pipe, that:...

  17. Cow's milk and children

    Science.gov (United States)

    Milk and children; Cow’s milk allergy - children; Lactose intolerance - children ... You may have heard that cow's milk should not be given to babies younger than 1 year old. This is because cow's milk doesn't provide enough of certain ...

  18. Association between antibody status to bovine herpesvirus 1 and quality of milk in dairy herds in Poland.

    Science.gov (United States)

    Rola, J G; Larska, M; Grzeszuk, M; Rola, J

    2015-02-01

    Bovine herpesvirus 1 (BoHV1) is one of the most important pathogens of cattle; however, its effect on somatic cell count and milk components is not completely understood. The aim of the current study was to examine the effect of BoHV1 infection on quality of bovine bulk tank milk (BTM). A total of 1,790 individual blood samples collected at 28 dairy farms were used to determine the BoHV1 infection status of the herds with ELISA tests. The quality parameters of milk were evaluated by instrumental methods with BTM samples collected at monthly intervals from May 2011 to May 2012. The statistical analysis was performed to study the associations between BoHV1 herd status, quality of BTM, and herd-specific parameters. The risk factors influencing bulk milk somatic cell count (BMSCC) were estimated using the multivariable mixed-effects maximum likelihood regression model. The true prevalences of BoHV1 infection at the animal and herd levels were 49.3 and 64.6%, respectively. The average BMSCC differed significantly between the herds grouped accordingly to their BoHV1 infection status. Interestingly, the highest BMSCC was observed in the vaccinated herds (240.3×10(3) cells/mL). Additionally, the BoHV1 herd status had a significant effect on the fat content of BTM. The largest herds that were investigated had a BoHV1 seroprevalence over 30%. The herd status was considerably influenced by the numbers of cows in the herds. Besides, no significant differences in total bacterial count or protein content in milk from BoHV1-infected und uninfected herds were observed. An increase in BMSCC was observed during summer compared with the winter months regardless of the BoHV1 status of the herds. In the final multivariable regression model, the main risk factors associated with BMSCC were BoHV1 herd status, the percentage of BoHV1 infected animals in a herd, the number of cows in a herd, and the season. Our study suggests that BoHV1 infection may influence BMSCC levels, which are key

  19. Tank 241-AW-101 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Sathyanarayana, P.

    1994-11-22

    The first section gives a summary of the available information for Tank AW-101. Included in the discussion are the process history and recent sampling events for the tank, as well as general information about the tank such as its age and the risers to be used for sampling. Tank 241-AW-101 is one of the 25 tanks on the Flammable Gas Watch List. To resolve the Flammable Gas safety issue, characterization of the tanks, including intrusive tank sampling, must be performed. Prior to sampling, however, the potential for the following scenarios must be evaluated: the potential for ignition of flammable gases such as hydrogen-air and/or hydrogen-nitrous oxide; and the potential for secondary ignition of organic-nitrate/nitrate mixtures in crust layer initiated by the burning of flammable gases or by a mechanical in-tank energy source. The characterization effort applicable to this Tank Characterization Plan is focused on the resolution of the crust burn flammable gas safety issue of Tank AW-101. To evaluate the potential for a crust burn of the waste material, calorimetry tests will be performed on the waste. Differential Scanning Calorimetry (DSC) will be used to determine whether an exothermic reaction exists.

  20. Tank characterization report for single-shell tank 241-S-104

    Energy Technology Data Exchange (ETDEWEB)

    DiCenso, A.T.; Simpson, B.C.

    1994-09-29

    In July and August 1992, Single-Shell Tank 241-S-104 was sampled as part of the overall characterization effort directed by the Hanford Federal Facility Agreement and Consent Order. Sampling was also performed to determine proper handling of the waste, to address corrosivity and compatibility issues, and to comply with requirements of the Washington Administrative Code. This Tank Characterization Report presents an overview of that tank sampling and analysis effort, and contains observations regarding waste characteristics. It also presents expected concentration and bulk inventory data for the waste contents based on this latest sampling data and background historical and surveillance tank information. Finally, this report makes recommendations and conclusions regarding operational safety. The purpose of this report is to describe the characteristics the waste in Single-Shell Tank 241-S-104 (hereafter, Tank 241-S-104) based on information obtained from a variety of sources. This report summarizes the available information regarding the chemical and physical properties of the waste in Tank 241-S-104, and using the historical information to place the analytical data in context, arranges this information in a format useful for making management and technical decisions concerning waste tank safety and disposal issues. In addition, conclusions and recommendations are presented based on safety issues and further characterization needs.

  1. Microbiological quality of goat's milk obtained under different production systems

    Directory of Open Access Journals (Sweden)

    J.K. Kyozaire

    2005-06-01

    Full Text Available In order to determine the safety of milk produced by smallholder dairy goat farms, a farm-based research study was conducted on commercial dairy goat farms to compare the microbiological quality of milk produced using 3 different types of dairy goat production systems (intensive, semi-intensive and extensive. A survey of dairy goat farms in and around Pretoria carried out by means of a questionnaire revealed that most of the smallholder dairy goat farms surveyed used an extensive type of production system. The method of milking varied with the type of production system, i.e. machine milking; bucket system machine milking and hand-milking, respectively. Udder half milk samples (n=270 were analysed, of which 31.1 % were infected with bacteria. The lowest intra-mammary infection was found amongst goats in the herd under the extensive system (13.3 %, compared with 43.3 % and 36.7 % infection rates under the intensive and semi-intensive production systems, respectively. Staphylococcus intermedius (coagulase positive, Staphylococcus epidermidis and Staphylococcus simulans (both coagulase negative, were the most common cause of intramammary infection with a prevalence of 85.7 % of the infected udder halves. The remaining 14.3 % of the infection was due to Staphylococcus aureus. Bacteriology of bulk milk samples on the other hand, showed that raw milk obtained by the bucket system milking machine had the lowest total bacterial count (16 450 colony forming units (CFU/mℓ compared to that by pipeline milking machine (36 300 CFU/mℓ or handmilking (48 000 CFU/mℓ. No significant relationship was found between the somatic cell counts (SCC and presence of bacterial infection in goat milk. In comparison with the herds under the other 2 production systems, it was shown that dairy goat farming under the extensive production system, where hand-milking was used, can be adequate for the production of safe raw goat milk.

  2. Theoretical comparison between solar combisystems based on bikini tanks and tank-in-tank solar combisystems

    DEFF Research Database (Denmark)

    Yazdanshenas, Eshagh; Furbo, Simon; Bales, Chris

    2008-01-01

    Theoretical investigations have shown that solar combisystems based on bikini tanks for low energy houses perform better than solar domestic hot water systems based on mantle tanks. Tank-in-tank solar combisystems are also attractive from a thermal performance point of view. In this paper......, theoretical comparisons between solar combisystems based on bikini tanks and tank-in-tank solar combisystems are presented....

  3. Tank characterization report: Tank 241-C-109

    International Nuclear Information System (INIS)

    Single-shell tank 241-C-109 is a Hanford Site Ferrocyanide Watch List tank that was most recently sampled in September 1992. Analyses of materials obtained from tank 241-C-109 were conducted to support the resolution of the ferrocyanide unreviewed safety question (USQ) and to support Hanford Federal Facility Agreement and consent Order (Tri- Party Agreement) Milestone M-10-00. This report describes this analysis

  4. Tank 244A tank characterization plan

    International Nuclear Information System (INIS)

    The Double-Shell Tank (DST) System currently receives waste from the Single-Shell Tank (SST) System in support of SST stabilization efforts or from other on-site facilities which generate or store waste. Waste is also transferred between individual DSTs. The mixing or commingling of potentially incompatible waste types at the Hanford Site must be addressed prior to any waste transfers into the DSTs. The primary goal of the Waste Compatibility Program is to prevent the formation of an Unreviewed Safety Question (USQ) as a result of improper waste management. Tank 244A is a Double Contained Receiver Tank (DCRT) which serves as any overflow tank for the East Area Farms. Waste material is able to flow freely between the underground storage tanks and tank 244A. Therefore, it is necessary to test the waste in tank 244A for compatibility purposes. Two issues related to the overall problem of waste compatibility must be evaluated: Assurance of continued operability during waste transfer and waste concentration and Assurance that safety problems are not created as a result of commingling wastes under interim storage. The results of the grab sampling activity prescribed by this Tank Characterization Plan shall help determine the potential for four kinds of safety problems: criticality, flammable gas accumulation, energetics, and corrosion and leakage

  5. Tank 241-B-101 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D. [Westinghouse Hanford Co., Richland, WA (United States)

    1995-04-28

    The Defense Nuclear Facilities Safety Board (DNFSB) has advised the US Department of Energy (DOE) to concentrate the near-term sampling and analysis activities on identification and resolution of safety issues (Conway 1993). The data quality objective (DQO) process was chosen as a tool to be used to identify the sampling and analytical needs for the resolution of safety issues. As a result, a revision in the Federal Facility Agreement and Consent Order (Tri-Party Agreement or TPA) milestone M-44 has been made, which states that ``A Tank Characterization Plan (TCP) will be developed for each double-shell tank (DST) and single-shell tank (SST) using the DQO process``. This document satisfies that requirement for tank 241-B-101 (B-101) sampling activities. Tank B-101 is identified as a low-heat load non-Watch List tank, and is classified as an assumed leaker. The tank is passively ventilated, interim stabilized, and intrusion prevention measures have been completed. As of January 31, 1995, approximately 428,000 liters of non-complexed waste was contained in the tank. Tank B-101 is expected to have two primary layers. A layer of saltcake waste generated from the 242-B evaporator, followed by a top layer of sludge composed of B-Plant high-level, B-Plant low-level, and unknown waste.

  6. Tank Characterization Report for Single Shell Tank 241-C-104

    Energy Technology Data Exchange (ETDEWEB)

    ADAMS, M.R.

    2000-04-06

    Interprets information about the tank answering a series of six questions covering areas such as information drivers, tank history, tank comparisons, disposal implications, data quality and quantity, and unique aspects of the tank.

  7. Cattle milk quality in the Semiarid region of Rio Grande do Norte, Brazil

    Directory of Open Access Journals (Sweden)

    Marcone Macêdo Tôrres Angicano

    2015-08-01

    Full Text Available The aim of this study was to evaluate the composition and quality of raw milk that was refrigerated in tanks, according to the farm structures, bovine diet, and production levels of the dairy producers that provide milk for the Association of Small-Scale Ranchers of the Angicos Wilderness (ASRAW. Data were collected from 47 dairy farmers from different cities of the state of Rio Grande do Norte who are associated with ASRAW. Twenty-three tanks were sampled throughout the month of June 2013, with two collections per week and five samples per tank, for a total of 920 samples. The properties were characterized according to the type of tank (private or community, the food provided during milk collection (voluminous or voluminous and concentrated, the structure of the dairy producer (family producer or non-family producer, and the amounts of each component. Analyses of fat content, total protein, casein levels, lactose levels, total solids, degreased dry extract (DDE, freezing point, urea levels, somatic cell counts (SCC, and antibiotic residues were performed. Statistical analyses were conducted for each group, and the means were compared by Tukey tests, with a critical significance level of 5% probability calculated with the PROC GLM procedure of the SAS® program. Most components differed significantly for each parameter. The highest difference was noted between the urea and SCC levels, which was probably due to the wide variety of dairy producer profiles. The SCC values exhibited the largest amplitude variations. Most milk components significantly differed according to the strata, producer, food, and tank. These changes probably had little influence on the animal’s physiology and productivity. Therefore, despite the small variations in the components of the milk supplied by ASRAW, the different types of milk storage tank, milk volume produced, and type of dairy producer affected the overall milk quality.

  8. [Cow's milk protein allergy through human milk].

    Science.gov (United States)

    Denis, M; Loras-Duclaux, I; Lachaux, A

    2012-03-01

    Cow's milk protein allergy (CMPA) is the first allergy that affects infants. In this population, the incidence rate reaches 7.5%. The multiplicity and aspecificity of the symptoms makes its diagnosis sometimes complicated, especially in the delayed type (gastrointestinal, dermatological, and cutaneous). CMPA symptoms can develop in exclusively breastfed infants with an incidence rate of 0.5%. It, therefore, raises questions about sensitization to cow's milk proteins through breast milk. Transfer of native bovine proteins such as β-lactoglobulin into the breast milk is controversial: some authors have found bovine proteins in human milk but others point to cross-reactivity between human milk proteins and cow's milk proteins. However, it seems that a small percentage of dietary proteins can resist digestion and become potentially allergenic. Moreover, some authors suspect the transfer of some of these dietary proteins from the maternal bloodstream to breast milk, but the mechanisms governing sensitization are still being studied. Theoretically, CMPA diagnosis is based on clinical observations, prick-test or patch-test results, and cow's milk-specific IgE antibody concentration. A positive food challenge test usually confirms the diagnosis. No laboratory test is available to make a certain diagnosis, but the detection of eosinophil cationic protein (ECP) in the mother's milk, for example, seems to be advantageous since it is linked to CMA. Excluding cow's milk from the mother's diet is the only cure when she still wants to breastfeed. Usually, cow's milk proteins are reintroduced after 6 months of exclusion. Indeed, the prognosis for infants is very good: 80% acquire a tolerance before the age of 3 or 4 years. Mothers should not avoid dairy products during pregnancy and breastfeeding as preventive measures against allergy. PMID:22226014

  9. Qualidade microbiológica do leite refrigerado nas fazendas Microbiological quality of refrigerated milk on farms

    Directory of Open Access Journals (Sweden)

    E.F. Arcuri

    2006-06-01

    Full Text Available Avaliaram-se a qualidade microbiológica do leite obtido mecanicamente e refrigerado durante 48 horas, em 24 rebanhos, e a associação entre a contaminação microbiana e os procedimentos de higienização dos equipamentos de ordenha e armazenamento do leite. Os procedimentos de higiene foram avaliados in loco com auxilio de questionários. Foram realizadas a contagem padrão em placas, a contagem de coliformes totais e a pesquisa de Staphylococcus aureus e Streptococcus agalactiae. No leite de 14 rebanhos, foram pesquisadas Salmonella spp. e Listeria monocytogenes. As médias geométricas da contagem padrão foram 10³ UFC/ml foram verificadas em sete rebanhos. S. aureus e S. agalactiae foram isolados em 22 e 12 dos 24 rebanhos, respectivamente, e não foram encontradas Salmonella spp. e L. monocytogenes. O uso de detergentes alcalino e ácido, mais o de sanitizante foi associado (P5×10(5 UFC/ml.The effect of bulk tank and milking machine cleaning procedures (determined from a questionnaire on bacterial contamination of 48-hour refrigerated milk was examined in 24 herds. Milk samples were analyzed for standard plate count (SPC and for total coliform, Staphylococcus aureus and Streptococcus agalactiae contamination. Milk samples from 14 herds were cultured for Salmonella and Listeria monocytogenes. Geometrical means of SPC were 10³ UFC/ml of total coliforms were observed in seven herds. S. aureus and S. agalactiae were found in 22 and 12 herds, respectively. Salmonella and L. monocytogenes were not found in any of the samples. The use of alkaline and acid detergents plus sanitizing was associated (P5.0×10(5 UFC/ml.

  10. Goat's milk: characteristics and possibility

    OpenAIRE

    Ljubica Tratnik; Rajka Božanić; Ida Drgalić

    2002-01-01

    Goat's milk today gets more and more attention. In comparison with other types of milk, the production of goat's milk increases the most. Although, the basic composition of goat's and cow's milk is very similar, goat's milk has an important dietetic and therapeutic advantages. In spite of that goat's milk is less explored. In this paper all main components of goat's milk and the somatic cells number are compared with cow's milk. Special emphasis is given on taste and therapeutic value of goat...

  11. Detection and enumeration of four foodborne pathogens in raw commingled silo milk in the United States.

    Science.gov (United States)

    Jackson, Emily E; Erten, Edibe S; Maddi, Neeraj; Graham, Thomas E; Larkin, John W; Blodgett, Robert J; Schlesser, Joseph E; Reddy, Ravinder M

    2012-08-01

    A nationwide survey was conducted to obtain qualitative and quantitative data on bacterial contamination of raw commingled silo milk intended for pasteurization. The levels of total aerobic bacteria, total coliforms, Enterobacteriaceae, Escherichia coli, and Staphylococcus aureus were determined using the TEMPO system. The prevalence rates and levels of presumptive Bacillus cereus, E. coli O157:H7, Listeria monocytogenes, and Salmonella spp. were determined in 214 samples. B. cereus was detected in 8.91% of samples, at 3.0 to 93 CFU/ml. E. coli O157:H7 was detected in 3.79 to 9.05% of samples, at bacteria were slightly lower in samples containing no pathogens. No correlation was observed between the levels of organisms detected with the TEMPO system and the presence or levels of any pathogen except E. coli O157:H7. A higher average log-transformed count of total viable bacteria was observed in samples positive for this organism. The high prevalence rates of target pathogens may be attributed to a variety of factors, including detection methods, sample size, and commingling of the milk in the silo. The effects of commingling likely contributed to the high prevalence rates and low levels of target pathogens because of the inclusion of milk from multiple bulk tanks. The high prevalence rates also may be the result of analysis of larger sample volumes using more sensitive detection methods. These quantitative data could be utilized to perform more accurate risk assessments and to better estimate the appropriate level of protection for dairy products and processing technologies.

  12. Selenium content of milk and milk products of Turkey. II.

    Science.gov (United States)

    Yanardağ, R; Orak, H

    1999-04-01

    Selenium content of 1028 milk and milk products of Turkey are presented in this study. The selenium content of human milk (colostrum, transitional, and mature milk), various kinds of milk [cow, sheep, goat, buffalo, paper boxes (3%, 1.5%, 0.012% fat), bottled milk, condensed milk (10% fat), mineral added milk (1.6%), and banana, strawberry, and chocolate milk] and milk products (kefir, yogurt, Ayran, various cheese, coffee cream, ice cream, butter, margarine, milk powder, and fruit yogurt) in Turkey were determined by a spectrofluorometric method. The selenium levels of cow milks collected from 57 cities in Turkey were also determined. Selenium levels in cow milk varied with geographical location in Turkey and were found to be lowest for Van and highest for Aksaray. The results [milk (cow, sheep, goat, buffalo and human) and milks products] were compared with literature data from different countries.

  13. Think tanks in Denmark

    DEFF Research Database (Denmark)

    Ørsten, Mark; Nørgaard Kristensen, Nete

    2016-01-01

    Though think tanks have a long history internationally, they have especially in recent years come to play an increasingly important role in both policy-formulation and public debate. In this article, we analyse the growing presence of think tanks in a Danish context during the 2000s and the first...... half of the 2010s, because in this national setting think tanks are still a relatively new phenomenon. Based on theories of mediatization and de-corporatization, we present 1) an analysis of the visibility of selected Danish think tanks in the media and 2) an analysis of their political networks...... outside the media. The study shows that the two largest and oldest think tanks in Denmark, the liberal think tank CEPOS and the social democratic think tank ECLM, are very active and observable in the media; that the media’s distribution of attention to these think tanks, to some extent, confirms a re...

  14. Tank 241-U-105 tank characterization plan

    International Nuclear Information System (INIS)

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, Oak Ridge National Laboratory, and PNL tank vapor program. The scope of this plan is to provide guidance for the sampling and analysis of vapor samples from tank 241-U-105

  15. Tank 241-TY-101 Tank Characterization Plan

    Energy Technology Data Exchange (ETDEWEB)

    Homi, C.S.

    1995-03-20

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, Oak Ridge National Laboratory, and PNL tank vapor program. The scope of this plan is to provide guidance for the sampling and analysis of vapor samples from tank 241-TY-101.

  16. 33 CFR Appendix A to Part 157 - Damage Assumptions, Hypothetical Outflows, and Cargo Tank Size and Arrangements

    Science.gov (United States)

    2010-07-01

    ... Outflows, and Cargo Tank Size and Arrangements A Appendix A to Part 157 Navigation and Navigable Waters... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Pt. 157, App. A Appendix A to Part 157... from tank vessels, three dimensions of the extent of damage of a parallelepiped on the side and...

  17. 33 CFR 157.110 - Crude Oil Washing Operations and Equipment Manual for foreign tank vessels: Submission.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Crude Oil Washing Operations and... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.110 Crude Oil Washing Operations and Equipment Manual for foreign tank...

  18. 33 CFR 157.108 - Crude Oil Washing Operations and Equipment Manual for U.S. tank vessels: Submission.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Crude Oil Washing Operations and... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.108 Crude Oil Washing Operations and Equipment Manual for U.S. tank...

  19. Alternative to decrease cholesterol in sheep milk cheeses.

    Science.gov (United States)

    Gómez-Cortés, P; Viturro, E; Juárez, M; de la Fuente, M A

    2015-12-01

    The presence of cholesterol in foods is of nutritional interest because high levels of this molecule in human plasma are associated with an increasing risk of cardiovascular disease and nowadays consumers are demanding healthier products. The goal of this experiment was to diminish the cholesterol content of Manchego, the most popular Spanish cheese manufactured from ewes milk. For this purpose three bulk milks coming from dairy ewe fed with 0 (Control), 3 and 6% of linseed supplement on their diet were used. Nine cheeses (3 per bulk milk) were manufactured and ripened for 3 months. Cholesterol of ewes milk cheese from 6% to 12% linseed supplemented diets decreased by 9.6% and 16.1% respectively, therefore supplying a healthier profile. In a second experiment, different sources of unsaturated fatty acids (rich in oleic, linoleic and α-linolenic acids) were supplemented to dairy ewes and no significant differences were found on cheese cholesterol levels. PMID:26041199

  20. Feed tank transfer requirements

    International Nuclear Information System (INIS)

    This document presents a definition of tank turnover; DOE responsibilities; TWRS DST permitting requirements; TWRS Authorization Basis (AB) requirements; TWRS AP Tank Farm operational requirements; unreviewed safety question (USQ) requirements; records and reporting requirements, and documentation which will require revision in support of transferring a DST in AP Tank Farm to a privatization contractor for use during Phase 1B

  1. Tank 241-AZ-101 tank characterization plan

    International Nuclear Information System (INIS)

    The Defense Nuclear Facilities Safety Board has advised the DOE to concentrate the near-term sampling and analysis activities on identification and resolution of safety issues. The Data Quality Objective (DQO) process was chosen as a tool to be used in the resolution of safety issues. As a result, A revision in the Federal Facilities Agreement and Consent Order (Tri-Party Agreement) milestone M-44 has been made, which states that ''A Tank Characterization Plan (TCP) will also be developed for each double-shell tank (DST) and single-shell tank (SST) using the DQO process. Development of TCPs by the DQO process is intended to allow users to ensure their needs will be met and that resources are devoted to gaining only necessary information''. This document satisfies that requirement for Tank 241-AZ-101 (AZ-101) sampling activities. Tank AZ-101 is currently a non-Watch List tank, so the only DQOs applicable to this tank are the safety screening DQO and the compatibility DQO, as described below. The contents of Tank AZ-101, as of October 31, 1994, consisted of 3,630 kL (960 kgal) of dilute non-complexed waste and aging waste from PUREX (NCAW, neutralized current acid waste). Tank AZ-101 is expected to have two primary layers. The bottom layer is composed of 132 kL of sludge, and the top layer is composed of 3,500 kL of supernatant, with a total tank waste depth of approximately 8.87 meters

  2. Tank 241-AZ-102 tank characterization plan

    International Nuclear Information System (INIS)

    The Defense Nuclear Facilities Safety Board has advised the DOE to concentrate the near-term sampling and analysis activities on identification and resolution of safety issues. The Data Quality Objective (DQO) process was chosen as a tool to be used in the resolution of safety issues. As a result, a revision in the Federal Facilities Agreement and Consent Order (Tri-Party Agreement) milestone M-44 has been made, which states that ''A Tank Characterization Plan (TCP) will also be developed for each double-shell tank (DST) and single-shell tank (SST) using the DQO process ... Development of TCPs by the DQO process is intended to allow users to ensure their needs will be met and that resources are devoted to gaining only necessary information''. This document satisfies that requirement for tank 241-AZ-102 (AZ-102) sampling activities. Tank AZ-102 is currently a non-Watch List tank, so the only DQOs applicable to this tank are the safety screening DQO and the compatibility DQO, as described below. The current contents of Tank AZ-102, as of October 31, 1994, consisted of 3,600 kL (950 kgal) of dilute non-complexed waste and aging waste from PUREX (NCAW, neutralized current acid waste). Tank AZ-102 is expected to have two primary layers. The bottom layer is composed of 360 kL of sludge, and the top layer is composed of 3,240 kL of supernatant, with a total tank waste depth of approximately 8.9 meters

  3. Cow's milk - infants

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/002448.htm Cow's milk - infants To use the sharing features on this ... old, you should not feed your baby cow's milk, according to the American Academy of Pediatrics (AAP). ...

  4. Breastfeeding and Breast Milk

    Science.gov (United States)

    ... Clinical Trials Resources and Publications Breastfeeding and Breast Milk: Condition Information Skip sharing on social media links Share this: Page Content Breastfeeding and Breast Milk: Condition Information​ ​​Breastfeeding, also called nursing, is the ...

  5. Milk-alkali syndrome

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/000332.htm Milk-alkali syndrome To use the sharing features on this page, please enable JavaScript. Milk-alkali syndrome is a condition in which there ...

  6. Milk Allergy in Infants

    Science.gov (United States)

    ... Story" 5 Things to Know About Zika & Pregnancy Milk Allergy in Infants KidsHealth > For Parents > Milk Allergy ... español Alergia a la leche en bebés About Milk Allergy Almost all infants are fussy at times. ...

  7. Milk demystified by chemistry.

    Science.gov (United States)

    Obladen, Michael

    2014-09-01

    This article traces the decline of milk from a heavenly elixir to a tradeable food. Early cultures regarded milk not as a simple nutrient, but a living fluid. Heroes and gods were believed to have been nurtured by animals after being abandoned. Character traits were assumed to be transmitted by milk; infantile diseases were attributed to "bad milk", whereas "good milk" was used as a remedy. With chemical methods developed at the end of the 18th century, it became known that human milk was higher in sugar and lower in protein than cow's milk. During the 19th century, "scientific" feeding emerged that meant modifying cow's milk to imitate the proportion of nutrients in human milk. In Boston from 1893, Rotch initiated the "percentage" method, requiring a physician's prescription. In Paris from 1894, Budin sterilized bottled infant milk. In Berlin in 1898, Rubner measured oxygen and energy uptake by calorimetry, prompting feeding by calories, and Czerny introduced regulated feeding by the clock. These activities ignored the emotional dimension of infant nutrition and the anti-infective properties of human milk. They may have also enhanced the decline in breastfeeding, which reached an all-time low in 1971. Milk's demystification made artificial nutrition safer, but paved the way for commercially produced infant formula.

  8. Special Milk Program

    Science.gov (United States)

    US Department of Agriculture, 2009

    2009-01-01

    The Special Milk Program provides milk to children in schools, child care institutions and eligible camps that do not participate in other Federal child nutrition meal service programs. The program reimburses schools and institutions for the milk they serve. In 2008, 4,676 schools and residential child care institutions participated, along with…

  9. Filling Tanks with Hydrazine

    Science.gov (United States)

    Krueger, K.

    2004-10-01

    At the Hydrazine workshop in 2002 in Noordwijk several presentations dealt with the filling of satellite tanks. I was a bit surprised about the amount of manpower that is needed for this work. But I saw the same during the filling of the SCA system tanks some years ago in Trauen/Germany. I want to present the work flow of filling RESUS Hydrazine tanks. This bladder tanks have a capacity of 64 litres and are similar to some of the satellite tanks. We fill this tanks 25 to 50 times a year. Although the specifications are not exactly the same as those for satellite tank filling, it might be interesting to see how this work can be done half-automatically, because handling with Hydrazine is not a nice job, and the faster it goes, the better.

  10. Think Tanks in Europe

    DEFF Research Database (Denmark)

    Kelstrup, Jesper Dahl

    The emergence of more think tanks in recent decades has spawned some interest in how they function and impact policy-making in the European Union and its member states. So far however few empirical studies of think tanks have been carried out and think tanks have mainly been studied...... in their national contexts. Questions regarding patterns and differences in think tank organisations and functions across countries have largely been left unanswered. This paper advances a definition and research design that uses different expert roles to categorise think tanks. A sample of 34 think tanks from...... Brussels, Denmark and Germany are categorised according to different expert roles in a pilot analysis. As the analysis is sensitive to the interpretation and weight given to different indicators, besides from picturing the think tank landscape, the analysis is intended to trigger a discussion of how...

  11. Commercial Submersible Mixing Pump For SRS Tank Waste Removal - 15223

    Energy Technology Data Exchange (ETDEWEB)

    Hubbard, Mike [Savannah River Remediation, LLC., Aiken, SC (United States); Herbert, James E. [Savannah River Remediation, LLC., Aiken, SC (United States); Scheele, Patrick W. [Savannah River Remediation, LLC., Aiken, SC (United States)

    2015-01-12

    product lubricated canned motor pumps designed to fit within available risers and have significant agitation capabilities to suspend waste solids. Waste removal and closure of two tanks has been accomplished with agitation provided by 3 SMPs installed within the tanks. In 2012, a team was assembled to investigate alternative solids removal technologies to support waste removal for closing tanks. The goal of the team was to find a more cost effective approach that could be used to replace the current mixing pump technology. This team was unable to identify an alternative technology outside of mixing pumps to support waste agitation and removal from SRS waste tanks. However, the team did identify a potentially lower cost mixing pump compared to the baseline SLPs and SMPs. Rather than using the traditional procurement using an engineering specification, the team proposed to seek commercially available submersible mixer pumps (CSMP) as alternatives to SLPs and SMPs. SLPs and SMPs have a high procurement cost and the actual cost of moving pumps between tanks has shown to be significantly higher than the original estimates that justified the reuse of SMPs and SLPs. The team recommended procurement of “off-the-shelf” industry pumps which may be available for significant savings, but at an increased risk of failure and reduced operating life in the waste tank. The goal of the CSMP program is to obtain mixing pumps that could mix from bulk waste removal through tank closure and then be abandoned in place as part of tank closure. This paper will present the development, progress and relative advantages of the CSMP.

  12. 49 CFR 174.63 - Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank car...

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank car tanks. 174.63 Section 174.63 Transportation Other....63 Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank...

  13. Bayesian estimation of test characteristics of real-time PCR, bacteriological culture and California mastitis test for diagnosis of intramammary infections with Staphylococcus aureus in dairy cattle at routine milk recordings.

    Science.gov (United States)

    Mahmmod, Yasser S; Toft, Nils; Katholm, Jørgen; Grønbæk, Carsten; Klaas, Ilka C

    2013-11-01

    Danish farmers can order a real-time PCR mastitis diagnostic test on routinely taken cow-level samples from milk recordings. Validation of its performance in comparison to conventional mastitis diagnostics under field conditions is essential for efficient control of intramammary infections (IMI) with Staphylococcus aureus (S. aureus). Therefore, the objective of this study was to estimate the sensitivity (Se) and specificity (Sp) of real-time PCR, bacterial culture (BC) and California mastitis test (CMT) for the diagnosis of the naturally occurring IMI with S. aureus in routinely collected milk samples using latent class analysis (LCA) to avoid the assumption of a perfect reference test. Using systematic random sampling, a total of 609 lactating dairy cows were selected from 6 dairy herds with bulk tank milk PCR cycle threshold (Ct) value ≤39 for S. aureus. At routine milk recordings, automatically obtained cow-level (composite) milk samples were analyzed by PCR and at the same milking, 2436 quarter milk samples were collected aseptically for BC and CMT. Results showed that 140 cows (23%) were positive for S. aureus IMI by BC while 170 cows (28%) were positive by PCR. Estimates of Se and Sp for PCR were higher than test estimates of BC and CMT. SeCMT was higher than SeBC however, SpBC was higher than SpCMT. SePCR was 91%, while SeBC was 53%, and SeCMT was 61%. SpPCR was 99%, while SpBC was 89%, and SpCMT was 65%. In conclusion, PCR has a higher performance than the conventional diagnostic tests (BC and CMT) suggesting its usefulness as a routine test for accurate diagnosis of S. aureus IMI from dairy cows at routine milk recordings. The use of LCA provided estimates of the test characteristics for two currently diagnostic tests (BC, CMT) and a novel technique (real-time PCR) for diagnosing S. aureus IMI under field conditions at routine milk recordings in Denmark.

  14. Bayesian estimation of test characteristics of real-time PCR, bacteriological culture and California mastitis test for diagnosis of intramammary infections with Staphylococcus aureus in dairy cattle at routine milk recordings.

    Science.gov (United States)

    Mahmmod, Yasser S; Toft, Nils; Katholm, Jørgen; Grønbæk, Carsten; Klaas, Ilka C

    2013-11-01

    Danish farmers can order a real-time PCR mastitis diagnostic test on routinely taken cow-level samples from milk recordings. Validation of its performance in comparison to conventional mastitis diagnostics under field conditions is essential for efficient control of intramammary infections (IMI) with Staphylococcus aureus (S. aureus). Therefore, the objective of this study was to estimate the sensitivity (Se) and specificity (Sp) of real-time PCR, bacterial culture (BC) and California mastitis test (CMT) for the diagnosis of the naturally occurring IMI with S. aureus in routinely collected milk samples using latent class analysis (LCA) to avoid the assumption of a perfect reference test. Using systematic random sampling, a total of 609 lactating dairy cows were selected from 6 dairy herds with bulk tank milk PCR cycle threshold (Ct) value ≤39 for S. aureus. At routine milk recordings, automatically obtained cow-level (composite) milk samples were analyzed by PCR and at the same milking, 2436 quarter milk samples were collected aseptically for BC and CMT. Results showed that 140 cows (23%) were positive for S. aureus IMI by BC while 170 cows (28%) were positive by PCR. Estimates of Se and Sp for PCR were higher than test estimates of BC and CMT. SeCMT was higher than SeBC however, SpBC was higher than SpCMT. SePCR was 91%, while SeBC was 53%, and SeCMT was 61%. SpPCR was 99%, while SpBC was 89%, and SpCMT was 65%. In conclusion, PCR has a higher performance than the conventional diagnostic tests (BC and CMT) suggesting its usefulness as a routine test for accurate diagnosis of S. aureus IMI from dairy cows at routine milk recordings. The use of LCA provided estimates of the test characteristics for two currently diagnostic tests (BC, CMT) and a novel technique (real-time PCR) for diagnosing S. aureus IMI under field conditions at routine milk recordings in Denmark. PMID:23992955

  15. Tank 241-T-111 characterization report

    International Nuclear Information System (INIS)

    In late October and early November of 1991, single-shell tank (SST) 241-T-111 (on the Organic Watch List) was sampled and analyses were conducted on the materials obtained to complete Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) Milestone M-10-00 to sample and analyze two cores from each tank. Other objectives that these measurements and inventory estimates support are as follows: Obtain estimates of both the concentration and total quantity of key analytes relating to safety issues, such as organics and radionuclides; Provided input to risk assessment-based disposal decisions for the waste; and Implement physical property measurements, such as rheology, bulk density, and particle size. These measurements are necessary for the design and fabrication of retrieval, pretreatment, and vitrification systems. The purpose of the tank characterization report is to describe and characterize the waste in SST 241-T-111, based on information from various sources. This report summarizes the available information regarding the waste in tank 241-T-111, and arranges this information in a format useful to support management and technical decisions concerning this waste tank

  16. Evaluation of an O antigen enzyme-linked immunosorbent assay for screening of milk samples for Salmonella dublin infection in dairy herds

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Lind, Peter; Bitsch, V.

    1995-01-01

    Levels of antibodies to the O antigens (0:1,9,12) of Salmonella dublin were tested in 1355 serum, 1143 cow milk and 160 bulk milk samples from dairy herds using an enzyme-linked immunosorbent assay (ELISA). In order to define the background reaction, milk samples from all lactating cows and serum...

  17. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL producing Enterobacteriaceae in food producing animals, minced meat and raw milk

    Directory of Open Access Journals (Sweden)

    Geser Nadine

    2012-03-01

    Full Text Available Abstract Background The impact of food animals as a possible reservoir for extended-spectrum beta-lactamase (ESBL producing Enterobacteriaceae, and the dissemination of such strains into the food production chain need to be assessed. In this study 334 fecal samples from pigs, cattle, chicken and sheep were investigated at slaughter. Additionally, 100 raw milk samples, representing bulk tank milk of 100 different dairy farms, 104 minced meat (pork and beef samples and 67 E. coli isolates from cattle E. coli mastitis were analyzed. Results As many as 15.3% of the porcine, 13.7% of the bovine, 8.6% of the sheep and 63.4% of the chicken fecal samples yielded ESBL producers after an enrichment step. In contrast, none of the minced meat, none of the bulk tank milk samples and only one of the mastitis milk samples contained ESBL producing strains. Of the total of 91 isolates, 89 were E. coli, one was Citrobacter youngae and one was Enterobacter cloacae. PCR analysis revealed that 78 isolates (85.7% produced CTX-M group 1 ESBLs while six isolates (6.6% produced CTX-M group 9 enzymes. Five detected ESBLs (5.5% belonged to the SHV group and 2 isolates (2.2% contained a TEM-type enzyme. A total of 27 CTX-M producers were additionally PCR-positive for TEM-beta-lactamase. The ESBL-encoding genes of 53 isolates were sequenced of which 34 produced CTX-M-1, 6 produced CTX-M-14, 5 produced CTX-M-15 and also 5 produced SHV-12. Two isolates produced TEM-52 and one isolate expressed a novel CTX-M group 1 ESBL, CTX-M-117. One isolate--aside from a CTX-M ESBL-- contained an additional novel TEM-type broad-spectrum beta-lactamase, TEM-186. Conclusions The relatively high rates of ESBL producers in food animals and the high genetic diversity among these isolates are worrisome and indicate an established reservoir in farm animals.

  18. Completion of the Operational Closure of Tank 18F and Tank 19F at the Savannah River Site by Grouting - 13236

    International Nuclear Information System (INIS)

    Radioactive waste is stored in underground waste tanks at the Savannah River Site (SRS). The low-level fraction of the waste is immobilized in a grout waste form, and the high level fraction is disposed of in a glass waste form. Once the waste is removed, the tanks are prepared for closure. Operational closure of the tanks consists of filling with grout for the purpose of chemically stabilizing residual material, filling the tank void space for long-term structural stability, and discouraging future intrusion. Two of the old-style single-shell tanks at the SRS have received regulatory approval confirming waste removal had been completed, and have been stabilized with grout as part of completing operational closure and removal from service. Consistent with the regulatory framework, two types of grout were used for the filling of Tanks 18F and 19F. Reducing grout was used to fill the entire volume of Tanks 18F and 19F (bulk fill grout) and a more flowable grout was used to fill equipment that was left in the tank (equipment fill grout). The reducing grout was added to the tanks using portable grout pumps filled from concrete trucks, and delivered the grout through slick lines to the center riser of each tank. Filling of the two tanks has been completed, and all equipment has been filled. The final capping of riser penetrations brings the operation closure of Tanks 18F and 19F to completion. (authors)

  19. 27 CFR 19.342 - Receipt and storage of bulk spirits and wines.

    Science.gov (United States)

    2010-04-01

    ... bulk spirits and wines. 19.342 Section 19.342 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Receipt and storage of bulk spirits and wines. (a) Deposit. All spirits entered for deposit in the storage... spirits or wines are being deposited in a partially filled tank in storage on bonded...

  20. Effects of storage on the major constituents of raw milk

    Directory of Open Access Journals (Sweden)

    Peter Zajác

    2015-10-01

    Full Text Available Milk testing and quality control should be carried out at all stages of the dairy chain. Milk can be tested for quantity, organoleptic characteristic, compositional characteristic, physical and chemical characteristics, hygienic characteristics, adulteration or drug residues. The content of the major constituents of raw milk is important for milk payment system. Enzymes naturally present in the milk can change the chemical composition of raw milk. Also, enzymes secreted by bacteria or enzymes from somatic cells can degrade the raw milk composition. Products of these degradation reactions can have undesirable effects on milk structure, smell and taste. It is very important that farm-fresh raw milk be cooled immediately to not more than 8 °C in the case of daily collection, or not more than 6 °C if collection is not daily. During transport the cold chain must be maintained. An authorized person, properly trained in the appropriate technique, shall perform sampling of bulk milk in farm. Laboratory samples should be dispatched immediately after sampling to the dairy company and consequently to the testing laboratory. The time for dispatch of the samples to the testing laboratory should be as short as possible, preferably within 24 h. Laboratory samples shall be transported and stored at temperature 1 to 5 °C. Higher temperatures may adversely affect the composition of the laboratory sample and may cause disputes between the farmer, the dairy company and the laboratory. The effect of refrigerated storage at temperature 4 °C during 24 h on the composition of raw milk were investigated in this work, because we wanted to know how the milk composition will be changed and how the laboratory results will be affected. In many cases, the samples are not preserved with chemical preservants like azidiol, bronopol, potassium dichromate or Microtabs. We found, that the composition of raw cows' milk after 24 was changed significantly (p >0.005. We found an

  1. Hanford tanks initiative plan

    Energy Technology Data Exchange (ETDEWEB)

    McKinney, K.E.

    1997-07-01

    Abstract: The Hanford Tanks Initiative (HTI) is a five-year project resulting from the technical and financial partnership of the U.S. Department of Energy`s Office of Waste Management (EM-30) and Office of Science and Technology Development (EM-50). The HTI project accelerates activities to gain key technical, cost performance, and regulatory information on two high-level waste tanks. The HTI will provide a basis for design and regulatory decisions affecting the remainder of the Tank Waste Remediation System`s tank waste retrieval Program.

  2. Hanford tanks initiative plan

    International Nuclear Information System (INIS)

    Abstract: The Hanford Tanks Initiative (HTI) is a five-year project resulting from the technical and financial partnership of the U.S. Department of Energy's Office of Waste Management (EM-30) and Office of Science and Technology Development (EM-50). The HTI project accelerates activities to gain key technical, cost performance, and regulatory information on two high-level waste tanks. The HTI will provide a basis for design and regulatory decisions affecting the remainder of the Tank Waste Remediation System's tank waste retrieval Program

  3. The importance of milk and milk products in human nutrition

    OpenAIRE

    Plisková, Gabriela

    2014-01-01

    Milk and dairy products are a pivotal part of human nutrition for all life. In this work all the important components of milk and then influence the consumption of milk and milk products on human health are evaluated. Milk is an important source of energy in the form of carbohydrates, proteins and fats, also substances for enhancing metabolism i.e. vitamins and minerals. This work also describes in details specific dairy products. Milk is very important immediately after birth, breast mil...

  4. Large area bulk superconductors

    Science.gov (United States)

    Miller, Dean J.; Field, Michael B.

    2002-01-01

    A bulk superconductor having a thickness of not less than about 100 microns is carried by a polycrystalline textured substrate having misorientation angles at the surface thereof not greater than about 15.degree.; the bulk superconductor may have a thickness of not less than about 100 microns and a surface area of not less than about 50 cm.sup.2. The textured substrate may have a thickness not less than about 10 microns and misorientation angles at the surface thereof not greater than about 15.degree.. Also disclosed is a process of manufacturing the bulk superconductor and the polycrystalline biaxially textured substrate material.

  5. 33 CFR 157.100 - Plans for U.S. tank vessels: Submission.

    Science.gov (United States)

    2010-07-01

    ... system that meets 46 CFR 56.01-10(d); (2) The design of each COW machine; (3) The arrangement, location... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.100 Plans for U.S....

  6. 33 CFR 157.102 - Plans for foreign tank vessels: Submission.

    Science.gov (United States)

    2010-07-01

    ... include— (a) A drawing or diagram of the COW pumping and piping system that meets 46 CFR 56.01-10(d); (b... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.102 Plans for...

  7. 46 CFR 153.981 - Leaving room in tank for cargo expansion.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Leaving room in tank for cargo expansion. 153.981 Section 153.981 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... Transfer Procedures § 153.981 Leaving room in tank for cargo expansion. The person in charge of...

  8. The minerals of milk

    OpenAIRE

    Gaucheron, Frédéric

    2005-01-01

    International audience The salt of milk constitutes a small part of milk (8-9 g*L-1); this fraction contains calcium, magnesium, sodium and potassium for the main cations and inorganic phosphate, citrate and chloride for the main anions. In milk, these ions are more or less associated between themselves and with proteins. Depending on the type of ion, they are diffusible (cases of sodium, potassium and chloride) or partially associated with casein molecules (cases of calcium, magnesium, ph...

  9. Effects of milk somatic cell counts on some physicochemical and functional characteristics of skim and whole milk powders.

    Science.gov (United States)

    Sert, Durmuş; Mercan, Emin; Aydemir, Serdar; Civelek, Mustafa

    2016-07-01

    The aim of this work was to study the influence of milk somatic cell count (SCC) levels on spray-dried milk powders. For this reason, 3 cow milks with different SCC (700,000 SCC/mL) were processed into skim (SMP) and whole milk powder (WMP). The effect of SCC on the physicochemical and functional characteristics of the milk powders and textural properties of set-type yogurts produced from reconstituted milk powders with different SCC was evaluated. A crucial difference was noted between milk powders depending on different SCC. Protein values and ash content of powder samples decreased correlatively with increasing SCC. The hydroxymethylfurfural content of SMP was higher than WMP. We noted an increase in hydroxymethylfurfural content of both SMP and WMP depending on elevated SCC. Solubility index of SMP and WMP was 1.280 to 1.632 and 0.940 to 1.208mL, respectively; with increasing SCC, solubility index was affected adversely. The highest foam stability was determined in SMP containing >700,000 SCC. Bulk density of SMP and WMP was between 0.682 and 0.708 and 0.660 to 0.685g/cm(3), respectively. An increase was observed in scorched particle of both SMP and WMP depending on increasing SCC. We found significant differences in particle size distribution of milk powders produced from milk with SCC at different levels. Although WMP had more uniform and big particle structure, SMP had more specific area. A negative correlation was noted between yogurt texture and SCC. Results indicate that milk SCC has negative influences on milk powder quality. PMID:27179852

  10. Comparison of Chamcham manufactured from cow milk and buffalo milk

    OpenAIRE

    Haque, M. A.; Rashid, M.H.; Kajal, M.F.I.; Istiak, M.S.

    2012-01-01

    This experiment was conducted to study quality of Chamcham manufactured from cow milk and to compare it with Chamcham manufactured from buffalo milk and mixture of cow and buffalo milk. Three types of Chamcham were prepared from cow milk(A), buffalo milk(B) and 50% cow +50% buffalo milk(C).In this experiment the quality of prepared Chamcham were evaluated with the help of chemical test. The moisture, total solids, protein, fat, ash and carbohydrate contents of cow milk and buffalo milk Chamch...

  11. Tank SY-102 remediation project: Flowsheet and conceptual design report

    International Nuclear Information System (INIS)

    The US Department of Energy established the Tank Waste Remediation System (TWRS) to safely manage and dispose of radioactive waste stored in underground tanks on the Hanford Site. A major program in TWRS is pretreatment which was established to process the waste prior to disposal. Pretreatment is needed to resolve tank safety issues and to separate wastes into high-level and low-level fractions for subsequent immobilization and disposal. There is a fixed inventory of actinides and fission products in the tank which must be prepared for disposal. By segregating the actinides and fission products from the bulk of the waste, the tank's contents can be effectively managed. Due to the high public visibility and environmental sensitivity of this problem, real progress and demonstrated efforts toward addressing it must begin as soon as possible. As a part of this program, personnel at the Los Alamos National Laboratory (LANL) have developed and demonstrated a flowsheet to remediate tank SY-102 which is located in the 200 West Area and contains high-level radioactive waste. This report documents the results of the flowsheet demonstrations performed with simulated, but radioactive, wastes using an existing glovebox line at the Los Alamos Plutonium Facility. The tank waste was characterized using both a tank history approach and an exhaustive evaluation of the available core sample analyses. This report also presents a conceptual design complete with a working material flow model, a major equipment list, and cost estimates

  12. Microbiological quality of milk in Tanzania: from Maasai stable to African consumer table.

    Science.gov (United States)

    Schoder, Dagmar; Maichin, Andreas; Lema, Benedict; Laffa, John

    2013-11-01

    In Tanzania, pastoralists such as the Maasai and small urban farmers are responsible for the country's milk production, and 95% of the national milk supply is sold without regulation. This study was conducted using hygiene checklists and milk sampling to investigate milk quality and safety at various steps throughout the milk production chain. In regions of Dar es Salaam and Lake Victoria, 196 milk samples were collected: 109 samples of raw milk, 41 samples of packed or open served heat-treated products, and 46 samples of fermented products. Samples were taken from (i) the production level (pastoralists and urban farmers), (ii) the collection level (middlemen and depots), (iii) processors (dairies), and (iv) retailers (kiosks). Samples were analyzed for hygiene criteria (total bacteria, total coliforms, Escherichia coli, and coagulase-positive staphylococci) and foodborne pathogens such as Salmonella, enterohemorrhagic E. coli O157:H7, and Listeria monocytogenes. Adequate heating of milk for drinking was determined via heat labile alkaline phosphatase and lactoperoxidase analysis. Total bacterial counts indicated that only 67% (73 of 109) of raw milk samples and 46% (19 of 41) of heat-treated samples met national Tanzanian standards. Bulk milk samples taken from the traditional milking vessels of Maasai pastoralists had the lowest total bacterial counts: ≥ 1 × 10(2) CFU/ml. Foodborne pathogens such as E. coli O157:H7 and Salmonella were isolated from 10.1% (11 of 109) of raw milk samples but were not detected in heat-treated or fermented products, and 83% of heat-treated milk samples were lactoperoxidase negative, indicating overpasteurization. Coliforms were detected in 41% (17 of 41) of processed milk samples, thus indicating a high rate of recontamination. A progressive decrease in microbial quality along the milk production chain was attributed to departures from traditional methods, inadequate milk containers, long transport distances, lack of cooling, and

  13. NMR-Based Milk Metabolomics

    OpenAIRE

    Bertram, Hanne C.; Larsen, Lotte B; Sundekilde, Ulrik K

    2013-01-01

    Milk is a key component in infant nutrition worldwide and, in the Western parts of the world, also in adult nutrition. Milk of bovine origin is both consumed fresh and processed into a variety of dairy products including cheese, fermented milk products, and infant formula. The nutritional quality and processing capabilities of bovine milk is closely associated to milk composition. Metabolomics is ideal in the study of the low-molecular-weight compounds in milk, and this review focuses on the ...

  14. A proteomic perspective on the changes in milk proteins due to high somatic cell count.

    Science.gov (United States)

    Zhang, L; Boeren, S; van Hooijdonk, A C M; Vervoort, J M; Hettinga, K A

    2015-08-01

    Although cows with subclinical mastitis have no difference in the appearance of their milk, milk composition and milk quality are altered because of the inflammation. To know the changes in milk quality with different somatic cell count (SCC) levels, 5 pooled bovine milk samples with SCC from 10(5) to 10(6) cells/mL were analyzed qualitatively and quantitatively using both one-dimension sodium dodecyl sulfate PAGE and filter-aided sample preparation coupled with dimethyl labeling, both followed by liquid chromatography tandem mass spectrometry. Minor differences were found on the qualitative level in the proteome from milk with different SCC levels, whereas the concentration of milk proteins showed remarkable changes. Not only immune-related proteins (cathelicidins, IGK protein, CD59 molecule, complement regulatory protein, lactadherin), but also proteins with other biological functions (e.g., lipid metabolism: platelet glycoprotein 4, butyrophilin subfamily 1 member A1, perilipin-2) were significantly different in milk from cows with high SCC level compared with low SCC level. The increased concentration of protease inhibitors in the milk with higher SCC levels may suggest a protective role in the mammary gland against protease activity. Prostaglandin-H2 D-isomerase showed a linear relation with SCC, which was confirmed with an ELISA. However, the correlation coefficient was lower in individual cows compared with bulk milk. These results indicate that prostaglandin-H2 D-isomerase may be used as an indicator to evaluate bulk milk quality and thereby reduce the economic loss in the dairy industry. The results from this study reflect the biological phenomena occurring during subclinical mastitis and in addition provide a potential indicator for the detection of bulk milk with high SCC. PMID:26094216

  15. Tank characterization reference guide

    International Nuclear Information System (INIS)

    Characterization of the Hanford Site high-level waste storage tanks supports safety issue resolution; operations and maintenance requirements; and retrieval, pretreatment, vitrification, and disposal technology development. Technical, historical, and programmatic information about the waste tanks is often scattered among many sources, if it is documented at all. This Tank Characterization Reference Guide, therefore, serves as a common location for much of the generic tank information that is otherwise contained in many documents. The report is intended to be an introduction to the issues and history surrounding the generation, storage, and management of the liquid process wastes, and a presentation of the sampling, analysis, and modeling activities that support the current waste characterization. This report should provide a basis upon which those unfamiliar with the Hanford Site tank farms can start their research

  16. Tank characterization reference guide

    Energy Technology Data Exchange (ETDEWEB)

    De Lorenzo, D.S.; DiCenso, A.T.; Hiller, D.B.; Johnson, K.W.; Rutherford, J.H.; Smith, D.J. [Los Alamos Technical Associates, Kennewick, WA (United States); Simpson, B.C. [Westinghouse Hanford Co., Richland, WA (United States)

    1994-09-01

    Characterization of the Hanford Site high-level waste storage tanks supports safety issue resolution; operations and maintenance requirements; and retrieval, pretreatment, vitrification, and disposal technology development. Technical, historical, and programmatic information about the waste tanks is often scattered among many sources, if it is documented at all. This Tank Characterization Reference Guide, therefore, serves as a common location for much of the generic tank information that is otherwise contained in many documents. The report is intended to be an introduction to the issues and history surrounding the generation, storage, and management of the liquid process wastes, and a presentation of the sampling, analysis, and modeling activities that support the current waste characterization. This report should provide a basis upon which those unfamiliar with the Hanford Site tank farms can start their research.

  17. Tank 18-F And 19-F Tank Fill Grout Scale Up Test Summary

    International Nuclear Information System (INIS)

    High-level waste (HLW) tanks 18-F and 19-F have been isolated from FTF facilities. To complete operational closure the tanks will be filled with grout for the purpose of: (1) physically stabilizing the tanks, (2) limiting/eliminating vertical pathways to residual waste, (3) entombing waste removal equipment, (4) discouraging future intrusion, and (5) providing an alkaline, chemical reducing environment within the closure boundary to control speciation and solubility of select radionuclides. This report documents the results of a four cubic yard bulk fill scale up test on the grout formulation recommended for filling Tanks 18-F and 19-F. Details of the scale up test are provided in a Test Plan. The work was authorized under a Technical Task Request (TTR), HLE-TTR-2011-008, and was performed according to Task Technical and Quality Assurance Plan (TTQAP), SRNL-RP-2011-00587. The bulk fill scale up test described in this report was intended to demonstrate proportioning, mixing, and transportation, of material produced in a full scale ready mix concrete batch plant. In addition, the material produced for the scale up test was characterized with respect to fresh properties, thermal properties, and compressive strength as a function of curing time.

  18. TANK 18-F AND 19-F TANK FILL GROUT SCALE UP TEST SUMMARY

    Energy Technology Data Exchange (ETDEWEB)

    Stefanko, D.; Langton, C.

    2012-01-03

    High-level waste (HLW) tanks 18-F and 19-F have been isolated from FTF facilities. To complete operational closure the tanks will be filled with grout for the purpose of: (1) physically stabilizing the tanks, (2) limiting/eliminating vertical pathways to residual waste, (3) entombing waste removal equipment, (4) discouraging future intrusion, and (5) providing an alkaline, chemical reducing environment within the closure boundary to control speciation and solubility of select radionuclides. This report documents the results of a four cubic yard bulk fill scale up test on the grout formulation recommended for filling Tanks 18-F and 19-F. Details of the scale up test are provided in a Test Plan. The work was authorized under a Technical Task Request (TTR), HLE-TTR-2011-008, and was performed according to Task Technical and Quality Assurance Plan (TTQAP), SRNL-RP-2011-00587. The bulk fill scale up test described in this report was intended to demonstrate proportioning, mixing, and transportation, of material produced in a full scale ready mix concrete batch plant. In addition, the material produced for the scale up test was characterized with respect to fresh properties, thermal properties, and compressive strength as a function of curing time.

  19. 46 CFR 154.195 - Aluminum cargo tank: Steel enclosure.

    Science.gov (United States)

    2010-10-01

    ... published in “Rules for Building and Classing Steel Vessels”, 1981. (c) The steel cover for the aluminum... 46 Shipping 5 2010-10-01 2010-10-01 false Aluminum cargo tank: Steel enclosure. 154.195 Section... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction...

  20. 46 CFR 154.188 - Membrane tank: Inner hull steel.

    Science.gov (United States)

    2010-10-01

    ... “Rules for Building and Classing Steel Vessels”, 1981. ... 46 Shipping 5 2010-10-01 2010-10-01 false Membrane tank: Inner hull steel. 154.188 Section 154.188... STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment...

  1. Fermented milk for hypertension

    DEFF Research Database (Denmark)

    Usinger, Lotte; Reimer, Christina; Ibsen, Hans

    2012-01-01

    Fermented milk has been suggested to have a blood pressure lowering effect through increased content of proteins and peptides produced during the bacterial fermentation. Hypertension is one of the major risk factors for cardiovascular disease world wide and new blood pressure reducing lifestyle...... interventions, such as fermented milk, would be of great importance....

  2. Herpesviruses and breast milk

    Directory of Open Access Journals (Sweden)

    C. Pietrasanta

    2014-06-01

    Full Text Available Breast milk has always been the best source of nourishment for newborns. However, breast milk can carry a risk of infection, as it can be contaminated with bacterial or viral pathogens. This paper reviews the risk of acquisition of varicella-zoster virus (VZV and cytomegalovirus (CMV, herpesviruses frequently detected in breastfeeding mothers, via breast milk, focusing on the clinical consequences of this transmission and the possible strategies for preventing it. Maternal VZV infections are conditions during which breastfeeding may be temporarily contraindicated, but expressed breast milk should always be given to the infant. CMV infection acquired through breast milk rarely causes disease in healthy term newborns; an increased risk of CMV disease has been documented in preterm infants. However, the American Academy of Pediatrics (AAP does not regard maternal CMV seropositivity as a contraindication to breastfeeding; according to the AAP, in newborns weighing less than 1500 g, the decision should be taken after weighing the benefits of breast milk against the risk of transmission of infection. The real efficacy of the different methods of inactivating CMV in breast milk should be compared in controlled clinical trials, rigorously examining the negative consequences that each of these methods can have on the immunological and nutritional properties of the milk itself, with a view to establish the best risk-benefit ratio of these strategies before they are recommended for use in clinical practice.

  3. Effect of prolonged storage on microbiological quality of raw milk

    Directory of Open Access Journals (Sweden)

    Bogdan Perko

    2011-06-01

    Full Text Available The changing of milk microbiota composition in collection tank due to two-day collection was investigated. Each out of 10 independent cycles of every two-day collection was sampled 4 times and microbiologically examined. PCA medium supplemented with skim milk powder was used to determine the total counts of mesophilic aerobic bacteria, proteolytic bacteria, psychrotrophic and proteolytic psychrotrophic bacteria, respectively. MRS and M17 media were used to determine the counts of lactobacilli and cocci (lactococci and enterococci, respectively. As expected the counts of microbial groups increased but particularly the ratio between the groups of microorganisms was changed. The most evident shift in composition of milk microbiota was detected for psychrotrophic, particularly proteolytic psychrotrophic bacteria that are of particular concern to the dairy industry. Proteolytic psychrotrophs are notorious for their proteolytic activities which adversely influence milk proteins. Moreover, secreted proteolytic enzymes cannot be destroyed by heat treatment processes that are normally used in milk processing. The counts of microbial groups sharply increased on the second day of two-day collection. From selected bacterial consortia, the presence of the individual group of microbes was also PCR examined. Results indicate that the microbiological quality of milk in a two-day collection system is remarkably lower in comparison with daily collection system.

  4. Tank 241-C-105 tank characterization plan

    International Nuclear Information System (INIS)

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, WHC 222-S Laboratory, and PNL 325 Analytical Chemistry Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples from tank 241-C-105

  5. Tank 241-TY-104 Tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D. [Westinghouse Hanford Co., Richland, WA (United States)

    1995-02-15

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-C Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-TY-104.

  6. Tank 241-TY-106 Tank Characterization Plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1995-02-22

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-S Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-TY-106.

  7. Tank 241-BX-103 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Bell, K.E. [Westinghouse Hanford Co., Richland, WA (United States)

    1995-04-21

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-S Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-BX-103.

  8. Tank 241-C-203: Tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1995-03-06

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-S Laboratory. Scope of this plan is to provide guidance for sampling and analysis of samples for tank 241-C-203.

  9. Tank 241-U-201 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1995-02-21

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 22-S Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-U-201.

  10. Tank 241-SX-115 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Sasaki, L.M.

    1995-04-24

    This document is a plan which serves as the contractual agreement between the Characterization Project, Sampling Operations, and WHC 222-S Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-SX-115.

  11. Tank 241-C-202: Tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1995-03-06

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-S Laboratory. Scope of this plan is to provide guidance for sampling and analysis of samples for tank 241-C-202.

  12. Tank 241-U-202 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1995-02-21

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, and WHC 222-S Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples for tank 241-U-202.

  13. Tank 241-C-101 tank characterization plan

    Energy Technology Data Exchange (ETDEWEB)

    Schreiber, R.D.

    1994-12-06

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, WHC 222-S Laboratory, and PNL 325 Analytical Chemistry Laboratory. The scope of this plan is to provide guidance for the sampling and analysis of samples from tank 241-C-101.

  14. Milk Production in Croatia

    Directory of Open Access Journals (Sweden)

    Petar Bosnić

    2003-09-01

    Full Text Available In the last few years Croatian economy is restructuring through the processes of market liberalization and closing to EU, which is demanding some significant changes. Agriculture is in the process of reforms on the basis of CAP (Common Agricultural Policy policies of the EU, and those changes are producing different effects in each agricultural sector. The most sensitive area is livestock production, especially cattle and milking cows (production of meat and milk. This sector has insufficient production. More precise, domestic production in Croatia can satisfy around 80% of one-year consumption. This study shows economic position of production and processing of milk with the emphasis on primary milk production, processing of milk, domestic market and export-import situation. The goal is to consider the situation, position and possibilities for development of this sector.

  15. The potential of Fourier transform infrared spectroscopy of milk samples to predict energy intake and efficiency in dairy cows.

    Science.gov (United States)

    McParland, S; Berry, D P

    2016-05-01

    Knowledge of animal-level and herd-level energy intake, energy balance, and feed efficiency affect day-to-day herd management strategies; information on these traits at an individual animal level is also useful in animal breeding programs. A paucity of data (especially at the individual cow level), of feed intake in particular, hinders the inclusion of such attributes in herd management decision-support tools and breeding programs. Dairy producers have access to an individual cow milk sample at least once daily during lactation, and consequently any low-cost phenotyping strategy should consider exploiting measureable properties in this biological sample, reflecting the physiological status and performance of the cow. Infrared spectroscopy is the study of the interaction of an electromagnetic wave with matter and it is used globally to predict milk quality parameters on routinely acquired individual cow milk samples and bulk tank samples. Thus, exploiting infrared spectroscopy in next-generation phenotyping will ensure potentially rapid application globally with a negligible additional implementation cost as the infrastructure already exists. Fourier-transform infrared spectroscopy (FTIRS) analysis is already used to predict milk fat and protein concentrations, the ratio of which has been proposed as an indicator of energy balance. Milk FTIRS is also able to predict the concentration of various fatty acids in milk, the composition of which is known to change when body tissue is mobilized; that is, when the cow is in negative energy balance. Energy balance is mathematically very similar to residual energy intake (REI), a suggested measure of feed efficiency. Therefore, the prediction of energy intake, energy balance, and feed efficiency (i.e., REI) from milk FTIRS seems logical. In fact, the accuracy of predicting (i.e., correlation between predicted and actual values; root mean square error in parentheses) energy intake, energy balance, and REI from milk FTIRS in

  16. Unexpected Decrease in Milk Production after Fenbendazole Treatment of Dairy Cows during Early Grazing Season.

    Science.gov (United States)

    Ravinet, Nadine; Chartier, Christophe; Bareille, Nathalie; Lehebel, Anne; Ponnau, Adeline; Brisseau, Nadine; Chauvin, Alain

    2016-01-01

    Gastrointestinal nematodes (GIN) infection can impair milk production (MP) in dairy cows. To investigate whether MP would be optimized by spring targeted-selective anthelmintic treatment in grazing cows, we assessed (1) the effect on MP of an anthelmintic treatment applied 1.5 to 2 months after turn-out, and (2) herd and individual indicators associated with the post-treatment MP response. A randomized controlled clinical trial was conducted in 13 dairy farms (578 cows) in western France in spring 2012. In each herd, lactating cows of the treatment group received fenbendazole orally, control cows remained untreated. Daily cow MP was recorded from 2 weeks before until 15 weeks after treatment. Individual serum pepsinogen and anti-Ostertagia antibody levels (expressed as ODR), faecal egg count and bulk tank milk (BTM) Ostertagia ODR were measured at treatment time. Anthelmintic treatment applied during the previous housing period was recorded for each cow. In each herd, information regarding heifers' grazing and anthelmintic treatment history was collected to assess the Time of Effective Contact (TEC, in months) with GIN infective larvae before the first calving. The effect of treatment on weekly MP averages and its relationships with herd and individual indicators were studied using linear mixed models with two nested random effects (cow within herd). Unexpectedly, spring treatment had a significant detrimental effect on MP (-0.92 kg/cow/day on average). This negative MP response was particularly marked in high producing cows, in cows not treated during the previous housing period or with high pepsinogen levels, and in cows from herds with a high TEC or a high BTM ODR. This post-treatment decrease in MP may be associated with immuno-inflammatory mechanisms. Until further studies can assess whether this unexpected result can be generalized, non-persistent treatment of immunized adult dairy cows against GIN should not be recommended in early grazing season. PMID

  17. Unexpected Decrease in Milk Production after Fenbendazole Treatment of Dairy Cows during Early Grazing Season.

    Directory of Open Access Journals (Sweden)

    Nadine Ravinet

    Full Text Available Gastrointestinal nematodes (GIN infection can impair milk production (MP in dairy cows. To investigate whether MP would be optimized by spring targeted-selective anthelmintic treatment in grazing cows, we assessed (1 the effect on MP of an anthelmintic treatment applied 1.5 to 2 months after turn-out, and (2 herd and individual indicators associated with the post-treatment MP response. A randomized controlled clinical trial was conducted in 13 dairy farms (578 cows in western France in spring 2012. In each herd, lactating cows of the treatment group received fenbendazole orally, control cows remained untreated. Daily cow MP was recorded from 2 weeks before until 15 weeks after treatment. Individual serum pepsinogen and anti-Ostertagia antibody levels (expressed as ODR, faecal egg count and bulk tank milk (BTM Ostertagia ODR were measured at treatment time. Anthelmintic treatment applied during the previous housing period was recorded for each cow. In each herd, information regarding heifers' grazing and anthelmintic treatment history was collected to assess the Time of Effective Contact (TEC, in months with GIN infective larvae before the first calving. The effect of treatment on weekly MP averages and its relationships with herd and individual indicators were studied using linear mixed models with two nested random effects (cow within herd. Unexpectedly, spring treatment had a significant detrimental effect on MP (-0.92 kg/cow/day on average. This negative MP response was particularly marked in high producing cows, in cows not treated during the previous housing period or with high pepsinogen levels, and in cows from herds with a high TEC or a high BTM ODR. This post-treatment decrease in MP may be associated with immuno-inflammatory mechanisms. Until further studies can assess whether this unexpected result can be generalized, non-persistent treatment of immunized adult dairy cows against GIN should not be recommended in early grazing season.

  18. Unexpected Decrease in Milk Production after Fenbendazole Treatment of Dairy Cows during Early Grazing Season.

    Science.gov (United States)

    Ravinet, Nadine; Chartier, Christophe; Bareille, Nathalie; Lehebel, Anne; Ponnau, Adeline; Brisseau, Nadine; Chauvin, Alain

    2016-01-01

    Gastrointestinal nematodes (GIN) infection can impair milk production (MP) in dairy cows. To investigate whether MP would be optimized by spring targeted-selective anthelmintic treatment in grazing cows, we assessed (1) the effect on MP of an anthelmintic treatment applied 1.5 to 2 months after turn-out, and (2) herd and individual indicators associated with the post-treatment MP response. A randomized controlled clinical trial was conducted in 13 dairy farms (578 cows) in western France in spring 2012. In each herd, lactating cows of the treatment group received fenbendazole orally, control cows remained untreated. Daily cow MP was recorded from 2 weeks before until 15 weeks after treatment. Individual serum pepsinogen and anti-Ostertagia antibody levels (expressed as ODR), faecal egg count and bulk tank milk (BTM) Ostertagia ODR were measured at treatment time. Anthelmintic treatment applied during the previous housing period was recorded for each cow. In each herd, information regarding heifers' grazing and anthelmintic treatment history was collected to assess the Time of Effective Contact (TEC, in months) with GIN infective larvae before the first calving. The effect of treatment on weekly MP averages and its relationships with herd and individual indicators were studied using linear mixed models with two nested random effects (cow within herd). Unexpectedly, spring treatment had a significant detrimental effect on MP (-0.92 kg/cow/day on average). This negative MP response was particularly marked in high producing cows, in cows not treated during the previous housing period or with high pepsinogen levels, and in cows from herds with a high TEC or a high BTM ODR. This post-treatment decrease in MP may be associated with immuno-inflammatory mechanisms. Until further studies can assess whether this unexpected result can be generalized, non-persistent treatment of immunized adult dairy cows against GIN should not be recommended in early grazing season.

  19. Tow Tank #1

    Science.gov (United States)

    1930-01-01

    Digging the channel for the Tow Tank. In the late 1920s, the NACA decided to investigate the aero/hydro dynamics of floats for seaplanes. A Hydrodynamics Branch was established in 1929 and special towing basin was authorized in March of that same year. Starr Truscott (the first head of the new division) described the tank in NACA TR 470: 'The N.A.C.A. tank is of the Froude type; that is, the model which is being tested is towed through still water at successive constant speeds from a carriage spanning the tank. At each constant speed the towing pull is measured, the trim and the rise, or change of draft, are recorded and, if the model is being towed at a fixed trim, the moment required to hold it there is measured and recorded.' 'The reinforced concrete basin containing the water has the following dimensions: (1) Length on water, extreme, 2,020 feet; (2) Normal width of water surface, 24 feet; (3) Normal depth of water, 12 feet; (4) Length of 12 foot depth, 1,980 feet.' The tank was dedicated on May 27, 1931. In 1936 the tank was extended to a total length of 2,960 feet. In 1959 the facility was turned over to the U.S. Navy.Published in NACA TR No. 470, 'The N.A.C.A. Tank: A High-Speed Towing Basin for Testing Models of Seaplane Floats,' by Starr Truscott, 1933.

  20. Determining concentration inhomogeneity inside liquid holding tanks

    International Nuclear Information System (INIS)

    Incorporating concentration measurements of special nuclear material (SNM) in a chemical processing accountability program requires uncertainty data for three types of errors: mixing, sampling, and analytical. Usually, the analytical measurement uncertainties - both random and systematic - are routinely determined for the laboratory instruments and procedures being used to measure the concentration of withdrawn liquid samples. However, little data currently exists to determine how representative the withdrawn liquid sample is in relation to the true average bulk concentration within the tank itself. This sample representativeness depends upon uncertainties associated with the tank mixing and sampling procedures. Both types of uncertainties are investigated. Mixing uncertainty or inhomogeneity has been found to be primarily a fluid mechanics problem while sampling uncertainty involves mostly chemistry. Experimental results are presented along with the early developments of a scaling analysis for future process design and accountability work. Tests were performed on full size pencil, double pencil, and annular tanks using saltwater solutions to achieve various levels of density stratifications within the tanks analogous to nitric acids solutions of different normalities

  1. Public health risk of some milk borne pathogens

    Directory of Open Access Journals (Sweden)

    Mohamed M.A. Zeinhom

    2014-09-01

    Full Text Available A total of 150 samples of raw milk, 75 each of farm and market milk were collected from different farms and supermarkets in Beni-Suef Governorate, in addition to 30 stool samples from milk handlers and 25 milker's hand swabs were examined for the presence of Escherichia coli, E. coli O157:H7, Salmonella, Aeromonas and Yersinia. Isolates were identified biochemically and serologically. The obtained results revealed that E. coli was detected in a percentage of 26.7% and 16% in the examined raw market and bulk farm milk respectively, while in stool and hand swabs samples were 16.6% and 16%, respectively. E. coli O157:H7 and Salmonella spp. failed to be detected in any of the examined samples. Additionally, 45% and 16.7% of the recovered E. coli strains from the examined raw market and farm milk samples were enteropathogenic O166, while 55% and 83.3 were untypable, respectively. On the other hand 60% of human stool samples isolates were O 148 and 40% of the isolates were untypable, while 100% of the hand swab isolates were untypable. The results also exhibits isolation rate of Aeromonas hydrophila in a percentage of 24%, 13.3%, 10% and 16% from market milk, farm milk samples, stool and hand swabs respectively. While Yersinea enterocolitica represent 3.3% in the stool samples only. The public health significance of isolated strains as well as suggested control measures were discussed.

  2. NACA Tow Tank

    Science.gov (United States)

    1930-01-01

    L4695 shows the interior view of construction of the Tow Tank. In the late 1920s, the NACA decided to investigate the aero/hydro dynamics of floats for seaplanes. A Hydrodynamics Branch was established in 1929 and special towing basin was authorized in March of that same year. Starr Truscott (the first head of the new division) described the tank in NACA TR 470: 'The N.A.C.A. tank is of the Froude type; that is, the model which is being tested is towed through still water at successive constant speeds from a carriage spanning the tank. At each constant speed the towing pull is measured, the trim and the rise, or change of draft, are recorded and, if the model is being towed at a fixed trim, the moment required to hold it there is measured and recorded.' 'The reinforced concrete basin containing the water has the following dimensions: (1) Length on water, extreme, 2,020 feet; (2) Normal width of water surface, 24 feet; (3) Normal depth of water, 12 feet; (4) Length of 12 foot depth, 1,980 feet.' This picture shows the tank before the coving was added. This brought the rails for the carriage closer together and helped suppress waves produced by the models. The finished tank would be filled with approximately 4 million gallons of salt water pumped in from the Back River. The tank was covered by a shelter which protected the water surface. The tank was dedicated on May 27, 1931. In 1936 the tank was extended to a total length of 2,960 feet. In 1959 the facility was turned over to the U.S. Navy.

  3. Iodine concentrations in milk of dairy cattle fed various amounts of iodine as ethylenediamine dihydroiodide.

    Science.gov (United States)

    Berg, J N; Padgitt, D; McCarthy, B

    1988-12-01

    Due to concerns about high I in milk, the dairy industry has proposed a voluntary standard of 500 micrograms of I/L as the maximum allowable I in milk sold for processing and human consumption. This study was undertaken to determine the amount of ethylenediamine dihydroiodide that could be fed to dairy cattle without exceeding this standard. Various amounts (0 to 45 mg/head per d) of the I compound were fed to a commercial dairy herd for 50 wk. Individual and bulk milk samples were analyzed for total iodine. Milk I in herd bulk milk was directly correlated (r = .92) with the amounts fed. However, the correlation of milk I of individual cows was not as high (r = .66), indicating some individual variation in metabolism and secretion of the I into the mammary gland. Milk production and number of lactations did not correlate with I in milk. Regression analysis indicated that 25 to 30 mg of ethylenediamine dihydroiodide per day can be fed to dairy cattle receiving a diet otherwise low in I without exceeding a 500 micrograms concentration in milk.

  4. 解读TANKED

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    品牌为重、创造为先,Tanked racing用不走“寻常路”给国内众多同行们生动地上了一课。通过持续近四年的高速成长,Tanked racingE成为中国头盔业翘楚,然而Tanked racing并不局限于此,因为未来的舞台还很大。

  5. Upgrading a 1950s tank farm to meet the environmental standards of the 1990S

    International Nuclear Information System (INIS)

    The Texaco Inc. Research and Development (Texaco) facility in Beacon, New York includes an above ground storage tank (AST) farm, known as Tank Farm No. 1, which consists of eighteen tanks with capacities ranging from 10,000 to 21,000 gallons. A second tank farm, at the Texaco, Beacon facility, designated as the Boiler House Tank Farm, includes three additional tanks with capacities from 10,000 to 44,900 gallons. The Tank Farm No. 1 AST systems are all vertical, carbon steel tanks which were initially installed in several phases in the 1950s. The Boiler House Tank Farm ASTs are also vertical, carbon steel tanks, including one riveted construction tank that was installed in 1931. Each of the Texaco ASTs are used to store a variety of petroleum products, including diesel fuel, stoddard solvent, used oil, and various grades of gasoline and gasoline components. The New York State Department of Environmental Conservation (NYSDEC) has established regulations for petroleum bulk storage in 6 NYCRR Parts 612 through 614. These regulations include requirements for monitoring and inspecting AST systems, including a rigorous ''out of service'' inspection, to be completed at least once every ten years. Although several revisions had been completed at Tank Farm No. 1 in recent years, including installation of a reinforced concrete secondary containment dike system and new above ground piping, the tank shells and most appurtenances (e.g. water drawoff valves), were unmodified since they were initially installed. On this basis, Texaco decided to upgrade the AST systems in conjunction with the NYSDEC ten-year inspections, by installing reinforced fiberglass liners in the tank floors, and by removing and/or replacing tank appurtenances to meet current industry standards and fire code requirements. This paper presents a summary of the program implemented to upgrade the Texaco, Beacon tank farm AST systems

  6. Research and design on measurable milk cans%可计量奶粉罐的研究与设计

    Institute of Scientific and Technical Information of China (English)

    周兰美

    2013-01-01

    In view of the milk powder package can not be directed measurement and easily polluting in using, a milk cans was designed. It's made of the lower tank, the upper tank and the metrology room in the upper tank. The milk is stored in the lower tank. An opening is arranged in the upper part of the tank and connected with the upper metrology room. When brewing the milk, open the lid of the milk can, upside down it in the bottle, then pull out the rope, the milk powder will automatically enter the bottle. Through the design calculation and analysis to determine the overall structure of the milk can and analyzing the causes that influence factors on inaccurate measurement and pollution. The designed milk can has the advantages of simple structure and accurate measurement.%针对现有奶粉包装不能直接计量,使用过程中容易污染等问题,设计一种可计量奶粉罐.它由下端罐体、上端罐体以及上端罐体中的计量室组成.奶粉储存在下端罐体中,在罐体的上端设置有开口,开口直接与上端的计量室相连.需要冲泡奶粉时,打开盖子,把奶粉罐倒扣在奶瓶上,然后拉动外面的绳子,奶粉就自动进入奶瓶中.通过计算分析确定奶粉罐的总体结构,分析影响计量不准确和容易污染的原因,该设计结构简单,可精确计量.

  7. Development of smart solar tanks

    DEFF Research Database (Denmark)

    Furbo, Simon; Andersen, Elsa

    1999-01-01

    The aim of the project is to develop smart solar tanks. A smart solar tank is a tank in which the domestic water can bee heated both by solar collectors and by an auxiliary energy supply system. The auxiliary energy supply system heats up the hot-water tank from the top and the water volume heated...

  8. Yoghurt fermentation trials utilizing mare milk: comparison with cow milk

    OpenAIRE

    R. Giangiacomo; M.C. Curadi; M Orlandi; Civardi, G; T.M.P. Cattaneo

    2011-01-01

    Mare milk shows a very interesting nutritional composition, similar to human milk. Whey protein fraction represents about 50% of total proteins, with a good amount of essential amino acids, and high lysozyme concentration (Jauregui-Adell, 1975). Mare milk contains essential fatty acids, progenitors of ω3 and ω6, higher than cow milk (Csapò et al., 1995; Curadi et al., 2002). In east european countries mare milk is utilized in dietetics and therapeutics for gastroenteric and...

  9. 7 CFR 1160.109 - Milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Milk. 1160.109 Section 1160.109 Agriculture Regulations... Orders; Milk), DEPARTMENT OF AGRICULTURE FLUID MILK PROMOTION PROGRAM Fluid Milk Promotion Order Definitions § 1160.109 Milk. Milk means any class of cow's milk produced in the United States....

  10. Plasmin: indigenous milk proteinase

    Directory of Open Access Journals (Sweden)

    Samir Kalit

    2002-06-01

    Full Text Available The most important characteristic of plasmin, as significant indigenous milk proteinase, its concentration, concentration measuring procedure and activity of plasmin are described. The most important factors, which have an influence on concentration and plasmin activity in milk, are stage of lactation and mastitis (high somatic cell count – SCC. In high SCC milk indigenous proteinase activity increased, especially in plasmin and plasminogen system.Specific hydrolytic activity of plasmin during primary proteolysis of some casein fractions is described. ß-CN is most susceptible fraction, but αs1-CN and αs2-Cn are less susceptible to degradation by plasmin. Almost all fractions of κ-CN are resistant to degradation by plasmin. Activation of plasminogen to plasmin is very complex biochemical process influenced by activators and inhibitors in milk, and can be increased in high SCC milk. There are many various types of inhibitors in milk serum and ßlactoglobulin is the most important after its thermal denaturation. Addition of aprotinin and soybean tripsin inhibitors in milk inhibits plasmin activity. Most important characteristic of plasmin is its thermostability onpasteurisation and even sterilisation. Mechanism of thermal inactivation of plasmin with developing covalent disulphide interaction between molecule of plasmin and serum proteins (mostly ß-laktoglobulin is described. Thermosensitive inhibitors of plasminogen activators and inhibitors of plasmin are inactivated by short pasteurisation and therefore increase plasmin activity,while higher temperature and longer treatment time inactivate plasmin activity.

  11. LIFE ESTIMATION OF HIGH LEVEL WASTE TANK STEEL FOR F-TANK FARM CLOSURE PERFORMANCE ASSESSMENT - 9310

    Energy Technology Data Exchange (ETDEWEB)

    Subramanian, K; Bruce Wiersma, B; Stephen Harris, S

    2009-01-12

    High level radioactive waste (HLW) is stored in underground carbon steel storage tanks at the Savannah River Site. The underground tanks will be closed by removing the bulk of the waste, chemical cleaning, heel removal, stabilizing remaining residuals with tailored grout formulations, and severing/sealing external penetrations. The life of the carbon steel materials of construction in support of the performance assessment has been completed. The estimation considered general and localized corrosion mechanisms of the tank steel exposed to grouted conditions. A stochastic approach was followed to estimate the distributions of failures based upon mechanisms of corrosion accounting for variances in each of the independent variables. The methodology and results used for one-type of tank is presented.

  12. Tank 48 - Chemical Destruction

    Energy Technology Data Exchange (ETDEWEB)

    Simner, Steven P.; Aponte, Celia I.; Brass, Earl A.

    2013-01-09

    Small tank copper-catalyzed peroxide oxidation (CCPO) is a potentially viable technology to facilitate the destruction of tetraphenylborate (TPB) organic solids contained within the Tank 48H waste at the Savannah River Site (SRS). A maturation strategy was created that identified a number of near-term development activities required to determine the viability of the CCPO process, and subsequent disposition of the CCPO effluent. Critical activities included laboratory-scale validation of the process and identification of forward transfer paths for the CCPO effluent. The technical documentation and the successful application of the CCPO process on simulated Tank 48 waste confirm that the CCPO process is a viable process for the disposition of the Tank 48 contents.

  13. Donor milk: current perspectives

    Directory of Open Access Journals (Sweden)

    Giuliani F

    2014-07-01

    Full Text Available Francesca Giuliani,1 Ilaria Rovelli,1 Chiara Peila,1 Stefania Alfonsina Liguori,2 Enrico Bertino,1 Alessandra Coscia1 1SCDU Neonatologia, Dipartimento di Scienze Pediatriche e dell'Adolescenza, Università degli Studi di Torino, Torino, Italy; 2SC Neonatologia, Ospedale Maria Vittoria, Torino, Italy Abstract: Mother's own milk is widely recognized as the optimal feeding for term infants, but increasing evidence exists of its benefits also for sick and preterm infants in neonatal intensive care units. However, the nutritional needs for appropriate growth and neurodevelopmental outcomes of such a particular population of infants should be attentively evaluated, considering also the indication to an appropriate fortification of human milk. The target is to achieve growth potential for preterm newborns while ensuring good metabolic outcomes and normal neurological development. When mother's milk is unavailable or in short supply, donor human milk (DHM represents the second best choice and, although somewhat modified by the Holder pasteurization process, it preserves many benefits when compared to formula, as documented by more and more reports, randomized controlled trials, and meta-analyses published in the past few years. Evidence exists of the protection exerted by DHM from necrotizing enterocolitis, while further studies are required to look at possible beneficial effects regarding infections, bronchopulmonary dysplasia, long-term cardiovascular risk factors, feeding tolerance, neurological outcome, and allergy. Finally, the concern that the use of DHM might decrease preterm infant breastfeeding is being raised. Conversely, publications exist showing that the use of DHM in the neonatal unit increases breastfeeding rates at discharge for infants of very low birth weight. Keywords: human milk, preterm infant feeding, milk bank, breast milk, mother's own milk, pasteurized human milk, fortification

  14. Bulk chemicals from biomass

    NARCIS (Netherlands)

    Haveren, van J.; Scott, E.L.; Sanders, J.P.M.

    2008-01-01

    Given the current robust forces driving sustainable production, and available biomass conversion technologies, biomass-based routes are expected to make a significant impact on the production of bulk chemicals within 10 years, and a huge impact within 20-30 years. In the Port of Rotterdam there is a

  15. TANK 5 SAMPLING

    Energy Technology Data Exchange (ETDEWEB)

    Vrettos, N; William Cheng, W; Thomas Nance, T

    2007-11-26

    Tank 5 at the Savannah River Site has been used to store high level waste and is currently undergoing waste removal processes in preparation for tank closure. Samples were taken from two locations to determine the contents in support of Documented Safety Analysis (DSA) development for chemical cleaning. These samples were obtained through the use of the Drop Core Sampler and the Snowbank Sampler developed by the Engineered Equipment & Systems (EES) group of the Savannah River National Laboratory (SRNL).

  16. Milk production, quality, and consumption in Jimma (Ethiopia): Facts and producers', retailers', and consumers' perspectives.

    Science.gov (United States)

    Tolosa, T; Verbeke, J; Piepers, S; Tefera, M; Getachew, Y; Supré, K; DeVliegher, S

    2016-02-01

    Four studies were performed to quantify milk production, quality and consumption in the town Jimma, Ethiopia. First, 47 dairy farmers and 44 milk retailers were interviewed to gain more insights in dairy farming and marketing, and associated constraints. Second, bulk milk samples (n=188) were collected for 4 consecutive weeks to investigate milk quality [Total Bacterial Counts (TBC), Coliform Counts (CC), Somatic Cell Counts (SCC), and antimicrobial residues]. Third, (bulk) milk samples from 32 farms, 46 milk retailers and the 3 local milk collection centers were collected to determine the presence of oxacillin susceptible-and oxacillin resistant Staphylococcus aureus. Fourth, 208 adult inhabitants were interviewed to gain more insight in milk consumption and associated concerns of consumers. The average dairy farm included in the studies consisted of 5 lactating cows, produced 43 liters of milk per day and was owned by male, literate adults. Milk was sold to retailers (71% of the production) and directly to customers (25%) without any quality control, whereas 4% was self-consumed. Shortage of animal nutrition and adulteration of the milk were the main constraints for farmers and retailers, respectively. The median TBC, CC and SCC were 122,500CFU/mL, 1,005CFU/mL and 609,500cells/mL, respectively. Antimicrobial residues were detected in 20% of all samples. In general, the milk quality was considered to be poor (TBC>10,000CFU/mL, and/or CC>100CFU/mL, and/or SCC>400,000cells/mL and/or presence of antimicrobial residues) in 97% of all samples. S. aureus was isolated from 12 (38%), 13 (33%), and 2 out of 3 of the milk samples originating from the dairy farms, the milk retailers, and the milk collection centers, respectively. Seven (26%) of the isolates were resistant to oxacillin suggesting the presence of MRSA (Lee, 2003). Local milk is occasionally consumed by adults but more frequently by children. Adults mainly drink spontaneously fermented milk (57% of 105

  17. Household Milk consumption and Its Socio-economic Associates in West Azarbayejan Province, North-west Iran

    Directory of Open Access Journals (Sweden)

    Fatemeh Esfarjani

    2015-06-01

    Full Text Available Background and Objectives: According to the available evidence, consumption of milk and other dairy products among Iranians is far less than recommendations. The share of different milks (i.e., traditionally vs. industrially processed and its associated variables are, however, neither consistent nor fully known in different Provinces.Materials and Methods: This cross-sectional study was conducted to determine household milk consumption and its association with selected socio-demographic factors in West Azarbayejan Province, North-west Iran. A total of 650 households were selected from urban and rural areas in three major Azeri and Kurdish districts (i.e. Urmia, Khoy and Mahabad using a multi-stage cluster sampling method. Data were collected using socio-economic and milk frequency questionnaires. Results: The findings indicated that traditionally-processed milk (bulk was the most common milk consumed at household level (62.5%. Mean of bulk milk consumption in urban and rural areas was 479±23 and 730±64 ml/wk per capita, respectively. It was also shown that establishment of the new food subsidization policy has decreased the mean of household milk consumption by approximately 3 l/wk in urban areas. Factor analysis detected a significant decrease in the higher tertiles of family size/ethnicity score consumption of both bulk and pasteurized milk, which resulted in decreased consumption of total milk. Conclusions: Designing and implementation of alternative approaches, such as targeted milk subsidies for poor households or vulnerable age-groups should be considered. Keywords: Household milk consumption, Milk processing method, Socio-economic variables, Iran.

  18. Cow's Milk Allergy

    DEFF Research Database (Denmark)

    Høst, Arne; Halken, Susanne

    2014-01-01

    Since the 1930's the scientific literature on cow's milk protein allergy (CMPA) has accumulated. Over the last decade new diagnostic tools and treatment approaches have been developed. The diagnosis of reproducible adverse reactions to cow's milk proteins (CMP), i.e. CMPA, still has to be confirmed...... by controlled elimination and challenge procedures. Advanced diagnostic testing using epitope and microarray technology may in the future improve the diagnostic accuracy of CMPA by determination of specific IgE against specific allergen components of cow's milk protein. The incidence of CMPA in early childhood...... with CMP. Milk from other mammals such as mare and donkey may be tolerated by some children with CMPA. Soy protein is as allergenic as CMP and soy formula is not recommended for young children with CMPA because of a great risk of development of allergy to soy, whereas soymilk is normally tolerated in older...

  19. Colostrum and milk production

    DEFF Research Database (Denmark)

    Quesnel, H; Farmer, Chantal; Theil, Peter Kappel

    2015-01-01

    for the sow. More specifically, fetal growth, mammary growth, colostrum production and sow maintenance require substantial amounts of nutrients during late gestation. After parturition, nutrients are mainly required for milk synthesis and sow maintenance, but the regressing uterus supplies considerable...... becomes catabolic due to the high priority of milk production and to current feeding practices. Indeed, feed is changed from a gestation to a lactation diet for most sows and the feed supply typically goes from a restricted supply to an ad libitum allowance. In addition, transition sows are often exposed...... to shifts in housing, and in Europe, this shift is now associated with a change from loose group housing to individual housing. Around parturition, colostrum is being secreted and milk synthesis is initiated in the mammary glands. After the onset of lactation, milk composition changes, especially during...

  20. Characteristics of breast milk and serology of women donating breast milk to a milk bank

    OpenAIRE

    Lindemann, P.; Foshaugen, I; Lindemann, R

    2004-01-01

    Objective: Breast milk is the most important nutrient to all newborn babies. If the mother's milk production is insufficient, it is important to provide donor breast milk without reduction of its immunologic and antimicrobial properties. Early use of breast milk to preterm infants has shown a reduced incidence of necrotising enterocolitis, a faster tolerance of enteral feeding, and a reduced need of parenteral nutrition. It is important to have milk from a CMV-IgG negative donor to VLBW infan...

  1. Residual contaminants in milk

    OpenAIRE

    Nevijo Zdolec; Mirza Hadžiosmanović; Lidija Kozačinski; Željka Cvrtila; Ivana Filipović

    2006-01-01

    Various chemical agents are used during the whole production chain of milk and dairy products. Production of feedingstuffs is accompanied with pesticide usage, which may remain in environment, thus are transported through feeding into animals, animal products and finally in human organism. Preparation procedure and storage conditions of feed also influence on milk safety in the sense of mycotoxins entering into the food chain. Chemical agents are, on daily basis, used on dairy farms either as...

  2. Milk oligosaccharides: a review

    OpenAIRE

    Oliveira, Diana; Wilbey, R. Andrew; Grandison, A S; Roseiro, Luísa

    2015-01-01

    Milk oligosaccharides (OSs) confer unique health benefits to the neonate. Although human digestive enzymes cannot degrade these sugars, they support specific commensal microbes and act as decoys to prevent the adhesion of pathogenic micro-organisms to gastrointestinal cells. The limited availability of human milk oligosaccharides (HMOs) impedes research into these molecules and their potential applications in functional food formulations. Recent studies show that complex OSs with fucose and N...

  3. Seasonal changes of some physical-chemical characteristics of milk from Dalmatian Pramenka ewes

    Directory of Open Access Journals (Sweden)

    Lidija Sajko-Matutinović

    2012-06-01

    Full Text Available The aim of this study was to evaluate the influence of year and season on milk fat content and physical properties (titratable acidity, pH value, freezing point, density of ewe’s milk. Total of 114 bulk milk samples were collected from six flocks of purebred Dalmatian Pramenka ewes located in Splitsko-dalmatinska county (surrounding of Sinj and Drniš during milking period (from early April till late August in 2009 and 2010. In all investigated flocks ewes were kept in similar (semi-extensive management system, with pasture dominated in summer ration, while meadow and alfalfa hay dominated in winter ration. Mean values of physicalchemical characteristics of milk were: milk fat content 6.96 %, titratable acidity 8.06 °SH, ionometric acidity 6.77 pH, freezing point -0.5611 °C, density 1.036 g/cm3. The year significantly affected the content of milk fat (P<0.05, as well as titratable acidity of milk (P<0.01. Significant influence of season on milk fat content (P<0.01 and all analyzed physical properties of milk (P <0.05 was determined.

  4. Milking Efficiency – A Milkability Trait for Automatically Milked Cows

    DEFF Research Database (Denmark)

    Løvendahl, Peter; Lassen, Jan; Chagunda, M G G

    Data from an experimental herd with automatic milkings from 486 first lactation cows were used to study alternative measures of milkability. One trait was milking efficiency, (kg milk per minute used in robot) the other “residual milking box time” using a linear regression to adjust daily time fo...... efficiency which showed only intermediate correlation. Both traits had weak correlations to somatic cell counts. It is concluded that either trait will be effective in selecting for cows giving more milk per minute occupying the milking robot, without increasing risk of mastitis...

  5. Bulk materials handling review

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-02-15

    The paper provides details of some of the most important coal handling projects and technologies worldwide. It describes development by Aubema Crushing Technology GmbH, Bedeschi, Cimbria Moduflex, DBT, Dynamic Air Conveying Systems, E & F Services, InBulk Technologies, Nord-Sen Metal Industries Ltd., Pebco Inc, Primasonics International Ltd., R.J.S. Silo Clean (International) Ltd., Takraf GmbH, and The ACT Group. 17 photos.

  6. Detecting β-Casein Variation in Bovine Milk

    Directory of Open Access Journals (Sweden)

    Anna Maria Caroli

    2016-01-01

    Full Text Available In bovine species, β-casein (β-CN is characterized by genetic polymorphism. The two most common protein variants are β-CN A2 (the original one and A1, differing from A2 for one amino acid substitution (Pro67 to His67. Several bioactive peptides affecting milk nutritional properties can originate from β-CN. Among them, β-casomorphin-7 (BCM7 ranging from amino acid 60 to 66 can be released more easily from β-CN variants carrying His67 (A1 type instead of Pro67 (A2 type. Nowadays, “A2 milk” is produced in different countries claiming its potential benefits in human health. The aim of this study was to further develop and apply an isoelectric focusing electrophoresis (IEF method to bulk and individual milk samples in order to improve its use for β-CN studies. We succeeded in identifying A2 milk samples correctly and quantifying the percentage of A2, A1, and B variants in bulk samples not derived from A2 milk as well as in individual milk samples. The method allows us to quantify the relative proportion of β-CN variants in whole milk without eliminating whey protein by acid or enzymatic precipitation of caseins. The aim of this study was also to study the different behavior of β-CN and β-lactoglobulin (β-LG in the presence of trichloroacetic acid (TCA. The higher sensitivity of β-CN to TCA allows quantifying β-CN variants after TCA fixation because β-LG is not visible. Monitoring β-CN variation in cattle breeds is important in order to maintain a certain balance between Pro67 and His67 in dairy products. Overall, the debate between A1 and A2 milk needs further investigation.

  7. Evaluation and implementation of good pratices in main points of microbiological contamination in milk production/
    Avaliação e implantação de boas práticas nos principais pontos de contaminação microbiológica na produção leiteira

    OpenAIRE

    Mykell S. Pereira; Luiz C. Silva; Emanuel R. Vacarelli; Douglas Furtado Magnani; Elsa H.W. Santana; Luís A. Nero; Ernst E. Muller; Márcia de A. F. Barros; Vanerli Beloti; Éder Paulo Fagan

    2005-01-01

    In order to improve the microbiological quality of Brazilian milk and dairy products some measures have been implemented in the milk-producing sector throughout the years, such as milk refrigeration and bulk collection. Refrigeration is a very efficient procedure, however allows psychrotrophics multiplication, which are largely found in milk produced in poor hygienic conditions. To assure and improve the microbiologic quality of milk turns out to be necessary the implementation of hygienic me...

  8. Bifidus milk powder: processing parameter standardization and shelf stability evaluation.

    Science.gov (United States)

    Selvamuthukumaran, Meenakshisundaram; Muthukumaran, Selva; Shukla, Shiv Shankar

    2016-04-01

    Spray dried bifidus milk powder was prepared by supplementing cow milk with different level of additives to obtain slurry of desired concentration. The slurry was pasteurized, cooled and inoculated with Bifidobacterium bifidum, incubated and dried to produce a bifidus milk powder. Among the various bifidus milk powder prepared, the slurry of mention the level total soluble solids exhibited good organoleptic characteristics and it has been standardized for further analysis. Moisture content, bulk density, insolubility index, hydroxymethyl furfural and thiobarbituric acid value of bifidus milk powder significantly increased, while the reflectance value significantly decreased during storage. The B. bifidum count significantly reduced and the bacterium were not detected at the end of the mention storage duration. As such the sentence is not acceptable in the abstract. The reconstituted bifidus milk powder was considered acceptable with an overall acceptability score of 6.97 on a nine-point Hedonic scale and showed a shelf stability of 120 days at ambient temperature condition (27 ± 2 °C). PMID:27413234

  9. Cattle Candidate Genes for Milk Production Traits

    OpenAIRE

    Kadlec, Tomáš

    2012-01-01

    The aim of this thesis is to make an overview of important candidate genes affecting milk yield and milk quality parameters, with an emphasis on genes associated with the quantity and quality of milk proteins and milk fat.

  10. C-tank transfers: Transuranic sludge removal from the C-1, C-2, and W-23 waste storage tanks at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Two fluidic pulse jet mixing systems were used to successfully mobilize remote-handled transuranic sludge for retrieval from three 50,000-gal horizontal waste storage tanks at Oak Ridge National Laboratory (ORNL). The results of this operation indicate that the pulse jet system should be considered for mixing and bulk retrieval of sludges in other vertical and horizontal waste tanks at ORNL and at other U.S. Department of Energy sites

  11. C-tank transfers: Transuranic sludge removal from the C-1, C-2, and W-23 waste storage tanks at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Dahl, T.L.; Lay, A.C.; Taylor, S.A.; Moore, J.W.

    1999-05-01

    Two fluidic pulse jet mixing systems were used to successfully mobilize remote-handled transuranic sludge for retrieval from three 50,000-gal horizontal waste storage tanks at Oak Ridge National Laboratory (ORNL). The results of this operation indicate that the pulse jet system should be considered for mixing and bulk retrieval of sludges in other vertical and horizontal waste tanks at ORNL and at other U.S. Department of Energy sites.

  12. Material selection for Multi-Function Waste Tank Facility tanks

    Energy Technology Data Exchange (ETDEWEB)

    Larrick, A.P.; Blackburn, L.D.; Brehm, W.F.; Carlos, W.C.; Hauptmann, J.P. [Westinghouse Hanford Co., Richland, WA (United States); Danielson, M.J.; Westerman, R.E. [Pacific Northwest Lab., Richland, WA (United States); Divine, J.R. [ChemMet Ltd., West Richland, WA (United States); Foster, G.M. [ICF Kaiser Hanford Co., Richland, WA (United States)

    1995-03-01

    This paper briefly summarizes the history of the materials selection for the US Department of Energy`s high-level waste carbon steel storage tanks. It also provides an evaluation of the materials for the construction of new tanks at the evaluation of the materials for the construction of new tanks at the Multi-Function Waste Tank Facility. The evaluation included a materials matrix that summarized the critical design, fabrication, construction, and corrosion resistance requirements: assessed. each requirement: and cataloged the advantages and disadvantages of each material. This evaluation is based on the mission of the Multi-Function Waste Tank Facility. On the basis of the compositions of the wastes stored in Hanford waste tanks, it is recommended that tanks for the Multi-Function Waste Tank Facility be constructed of ASME SA 515, Grade 70, carbon steel.

  13. Milk Enhancements Improve Milk Consumption and Increase Meal Participation in the NSLP: The School Milk Pilot Test

    Science.gov (United States)

    Rafferty, Karen; Zipay, Diane; Patey, Camellia; Meyer, Jennifer

    2009-01-01

    Purpose/Objectives: The objective of the School Milk Pilot Test and the Westside School Milk Pilot Study was to test the effect of a milk enhancement initiative to make milk more appealing and attractive to elementary and secondary school students and to improve milk consumption. Methods: 146 schools participated in the national School Milk Pilot…

  14. Dwelling Water Tanks in Diyarbakir

    Directory of Open Access Journals (Sweden)

    Ali Ceylan

    2008-02-01

    Full Text Available BACKGROUND: In this connection, the object of this study has been to identify and compare the microbiological contamination and residue chlorine levels in the main network water that is taken from the Dicle Dam and distributed in Diyarbakir Province Centre and in the tanks of dwellings that use this water as well as the effects of the maintenance, hygiene, and physical conditions of these tanks on microbiological contamination. METHODS: Water samples were taken from both the tank input side network water and tank output side tank waters of 200 dwellings with water tanks in Diyarbakir city centre (tank entrance network side water for 200 and tank output side tank water for 200 within the framework of the research study. RESULTS: Coliform bacteria were detected in 35% of the tank entrance side network water samples and in 52.0% percent of the tank output side water samples. Faecal coliform bacteria were not detected in tank entrance side network water samples, but they existed in 2.5% of the tank output side water samples. Free residue chlorine level was found to be over 0.2 ppm in 67% of tank entrance side network water samples and in 35% of the tank output side water samples. Coliform bacteria were detected in 95.5% of the tank entrance side network water samples, of which free residue chlorine level were below 0.2 ppm. Total germ growth was detected in 52.0% of the tank entrance side network water samples and in 67.5% of the tank output side water samples. The most frequently isolated bacteria both in tank entrance side network and tank output side water samples were found to be Bacillus spp. Bacillus type bacteria were found in 48% of tank entrance side network water samples and 57.5% of the tank output side water samples. Filamentous fungi were prevalent in 8% of all the samples examined within the study and the most commonly isolated filamentous fungi were Aspergillus spp (5.5% and Penicillum spp (2.5%. Water tanks of dwellings contain more

  15. Organic Milk Quality in the Netherlands : Distinguishable from conventional milk?

    NARCIS (Netherlands)

    Hospers-Brands, A.J.T.M.; Burgt, van der G.J.H.M.

    2009-01-01

    Recent studies have indicated possible positive interactions between organic animal production and, particularly, and various vitamins. As possible distinguishing quality parameters for organic milk, the differences between organic and conventional milk in Netherlands for fatty acid composition and

  16. A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk.

    Directory of Open Access Journals (Sweden)

    Elisabetta Casarin

    Full Text Available Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire herds as "disease-free". However, diagnostic tests for pooled samples need to be particularly sensitive, especially when the levels of disease markers are low, as in the case of anti-BoHV1 antibodies in milk as markers of Infectious Bovine Rhinotracheitis (IBR disease. The avidin-nucleic-acid-nanoassembly (ANANAS is a novel kind of signal amplification platform for immunodiagnostics based on colloidal poly-avidin nanoparticles that, using model analytes, was shown to strongly increase ELISA test performance as compared to monomeric avidin. Here, for the first time, we applied the ANANAS reagent integration in a real diagnostic context. The monoclonal 1G10 anti-bovine IgG1 antibody was biotinylated and integrated with the ANANAS reagents for indirect IBR diagnosis from pooled milk mimicking tank samples from herds with IBR prevalence between 1 to 8%. The sensitivity and specificity of the ANANAS integrated method was compared to that of a classic test based on the same 1G10 antibody directly linked to horseradish peroxidase, and a commercial IDEXX kit recently introduced in the market. ANANAS integration increased by 5-fold the sensitivity of the 1G10 mAb-based conventional ELISA without loosing specificity. When compared to the commercial kit, the 1G10-ANANAS integrated method was capable to detect the presence of anti-BHV1 antibodies from bulk milk of gE antibody positive animals with 2-fold higher sensitivity and similar specificity. The results demonstrate the potentials of this new amplification technology, which permits improving current classic ELISA

  17. A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk.

    Science.gov (United States)

    Casarin, Elisabetta; Lucchese, Laura; Grazioli, Santina; Facchin, Sonia; Realdon, Nicola; Brocchi, Emiliana; Morpurgo, Margherita; Nardelli, Stefano

    2016-01-01

    Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire herds as "disease-free". However, diagnostic tests for pooled samples need to be particularly sensitive, especially when the levels of disease markers are low, as in the case of anti-BoHV1 antibodies in milk as markers of Infectious Bovine Rhinotracheitis (IBR) disease. The avidin-nucleic-acid-nanoassembly (ANANAS) is a novel kind of signal amplification platform for immunodiagnostics based on colloidal poly-avidin nanoparticles that, using model analytes, was shown to strongly increase ELISA test performance as compared to monomeric avidin. Here, for the first time, we applied the ANANAS reagent integration in a real diagnostic context. The monoclonal 1G10 anti-bovine IgG1 antibody was biotinylated and integrated with the ANANAS reagents for indirect IBR diagnosis from pooled milk mimicking tank samples from herds with IBR prevalence between 1 to 8%. The sensitivity and specificity of the ANANAS integrated method was compared to that of a classic test based on the same 1G10 antibody directly linked to horseradish peroxidase, and a commercial IDEXX kit recently introduced in the market. ANANAS integration increased by 5-fold the sensitivity of the 1G10 mAb-based conventional ELISA without loosing specificity. When compared to the commercial kit, the 1G10-ANANAS integrated method was capable to detect the presence of anti-BHV1 antibodies from bulk milk of gE antibody positive animals with 2-fold higher sensitivity and similar specificity. The results demonstrate the potentials of this new amplification technology, which permits improving current classic ELISA sensitivity limits

  18. Inerting ballast tanks

    Energy Technology Data Exchange (ETDEWEB)

    Baes, Gabriel L.; Bronneberg, Jos [SBM Offshore, AA Schiedam (Netherlands); Barros, Maria A.S.D. de [Universidade Estadual de Maringa (UEM), PR (Brazil)

    2012-07-01

    This report expands upon the work conducted by SBM Offshore to develop a tank preservation treatment, which is intended to achieve a service life of 30 years. This work focuses on the corrosion problems, in the ballast tanks, based on new built hulls, both for the Gas Exploration Market, the FLNG - Floating Liquefied Natural Gas, and for the Oil Exploration market - FPSO's - Floating Production Storage and offloading Units. Herein, the corrosion rate input comes from the various references related to the process of nitrogen injection, which is expected to extend the vessel's time life. The essential elements of this solution comprise the deoxygenation process, corrosion models, coating effects, tests from laboratory, shipboard tests, corrosion institutes and regulations applicable to the operation. The best corrosion protection system for ballast tanks area combines a coating system and an inert gas system. The condition of the tanks will be dependent upon the level of protection applied to the steel structure, including, but not limited to coating, cathodic protection, etc. There is a need for products which extend the life time. It is not sufficient, only have good theoretical base for the corrosion and an excellent treatment system. In addition, the design of the ships structure must also eliminate the presence of local stress concentrations which can result in fatigue cracking and rupture of the protective coating barrier starting the corrosion. As a direct result of this, more problems in corrosion can be mitigated, vessels can have a better corrosion performance with less maintenance and repairs to coating systems in ballast tanks. Furthermore ships will be positively impacted operationally due to less frequent dry docking. There is a huge potential in the application of inert gas to combat the corrosion rate inside the ballast tanks, one of the most corrosive environments on earth. This application can have a direct impact on vessel structure

  19. How to reintroduce cow's milk?

    Science.gov (United States)

    Dupont, Christophe

    2013-11-01

    In a child that is allergic to milk, the natural next step, following the elimination diet, is the reintroduction of cow's milk. Several questions may arise. When feasible, this reintroduction has many benefits for the child and his family. However, the disease needs to be well defined by physicians and explained to parents. They need to understand that there are different types of allergy to cow's milk, specifically IgE- and non-IgE-mediated, and each of these may exhibit both a variable duration and frequently an incomplete recovery. Deciding where to first reintroduce cow's milk to a child who has previously followed a milk-free diet, whether it be at home or in a hospital, also frequently presents an issue. Following this first reintroduction, the progressive increase of milk into the diet needs to be managed properly, as not all children will go back to a normal dairy products intake. Recent studies show that most children with milk allergy tolerate products containing baked milk and that their consumption might speed up recovery. Hence, the purpose of the milk challenge in a child on a milk-free diet is becoming, even in a child still reactive to milk, the first step of gradual and individually adapted reintroduction of milk or dairy products. When reintroduction of cow's milk does not work, immunotherapy becomes an option, and this is carried out in specialized centers.

  20. NMR-Based Milk Metabolomics

    Directory of Open Access Journals (Sweden)

    Hanne C. Bertram

    2013-04-01

    Full Text Available Milk is a key component in infant nutrition worldwide and, in the Western parts of the world, also in adult nutrition. Milk of bovine origin is both consumed fresh and processed into a variety of dairy products including cheese, fermented milk products, and infant formula. The nutritional quality and processing capabilities of bovine milk is closely associated to milk composition. Metabolomics is ideal in the study of the low-molecular-weight compounds in milk, and this review focuses on the recent nuclear magnetic resonance (NMR-based metabolomics trends in milk research, including applications linking the milk metabolite profiling with nutritional aspects, and applications which aim to link the milk metabolite profile to various technological qualities of milk. The metabolite profiling studies encompass the identification of novel metabolites, which potentially can be used as biomarkers or as bioactive compounds. Furthermore, metabolomics applications elucidating how the differential regulated genes affects milk composition are also reported. This review will highlight the recent advances in NMR-based metabolomics on milk, as well as give a brief summary of when NMR spectroscopy can be useful for gaining a better understanding of how milk composition is linked to nutritional or quality traits.

  1. NMR-Based Milk Metabolomics.

    Science.gov (United States)

    Sundekilde, Ulrik K; Larsen, Lotte B; Bertram, Hanne C

    2013-01-01

    Milk is a key component in infant nutrition worldwide and, in the Western parts of the world, also in adult nutrition. Milk of bovine origin is both consumed fresh and processed into a variety of dairy products including cheese, fermented milk products, and infant formula. The nutritional quality and processing capabilities of bovine milk is closely associated to milk composition. Metabolomics is ideal in the study of the low-molecular-weight compounds in milk, and this review focuses on the recent nuclear magnetic resonance (NMR)-based metabolomics trends in milk research, including applications linking the milk metabolite profiling with nutritional aspects, and applications which aim to link the milk metabolite profile to various technological qualities of milk. The metabolite profiling studies encompass the identification of novel metabolites, which potentially can be used as biomarkers or as bioactive compounds. Furthermore, metabolomics applications elucidating how the differential regulated genes affects milk composition are also reported. This review will highlight the recent advances in NMR-based metabolomics on milk, as well as give a brief summary of when NMR spectroscopy can be useful for gaining a better understanding of how milk composition is linked to nutritional or quality traits. PMID:24957988

  2. TANK SPACE OPTIONS REPORT

    Energy Technology Data Exchange (ETDEWEB)

    WILLIS WL; AHRENDT MR

    2009-08-11

    Since this report was originally issued in 2001, several options proposed for increasing double-shell tank (DST) storage space were implemented or are in the process of implementation. Changes to the single-shell tank (SST) waste retrieval schedule, completion of DST space saving options, and the DST space saving options in progress have delayed the projected shortfall of DST storage space from the 2007-2011 to the 2018-2025 timeframe (ORP-11242, River Protection Project System Plan). This report reevaluates options from Rev. 0 and includes evaluations of new options for alleviating projected restrictions on SST waste retrieval beginning in 2018 because of the lack of DST storage space.

  3. TANK SPACE OPTIONS REPORT

    International Nuclear Information System (INIS)

    Since this report was originally issued in 2001, several options proposed for increasing double-shell tank (DST) storage space were implemented or are in the process of implementation. Changes to the single-shell tank (SST) waste retrieval schedule, completion of DST space saving options, and the DST space saving options in progress have delayed the projected shortfall of DST storage space from the 2007-2011 to the 2018-2025 timeframe (ORP-11242, River Protection Project System Plan). This report reevaluates options from Rev. 0 and includes evaluations of new options for alleviating projected restrictions on SST waste retrieval beginning in 2018 because of the lack of DST storage space.

  4. Tank waste isotope contributions

    International Nuclear Information System (INIS)

    This document presents the results of a calculation to determine the relative contribution of selected isotopes to the inhalation and ingestion doses for a postulated release of Hanford tank waste. The fraction of the dose due to 90Sr, 90Y, 137Cs and the alpha emitters for single shell solids and liquids, double shell solids and liquids, aging waste solids and liquids and all solids and liquids. An effective dose conversion factor was also calculated for the alpha emitters for each composite of the tank waste

  5. 7 CFR 1001.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1001.13 Section 1001.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE NORTHEAST MARKETING AREA Order Regulating Handling Definitions § 1001.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  6. 7 CFR 1131.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1131.13 Section 1131.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE ARIZONA MARKETING AREA Order Regulating Handling Definitions § 1131.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  7. 7 CFR 58.137 - Excluded milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Excluded milk. 58.137 Section 58.137 Agriculture... Milk § 58.137 Excluded milk. A plant shall not accept milk from a producer if: (a) The milk has been in...) Three of the last five milk samples have exceeded the maximum bacterial estimate of 500,000 per...

  8. 7 CFR 1032.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1032.13 Section 1032.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE CENTRAL MARKETING AREA Order Regulating Handling Definitions § 1032.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  9. 7 CFR 1006.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1006.13 Section 1006.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE FLORIDA MARKETING AREA Order Regulating Handling Definitions § 1006.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  10. 7 CFR 1033.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1033.13 Section 1033.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE MIDEAST MARKETING AREA Order Regulating Handling Definitions § 1033.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  11. 7 CFR 1126.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1126.13 Section 1126.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE SOUTHWEST MARKETING AREA Order Regulating Handling Definitions § 1126.13 Producer milk. Producer milk means the skim milk (or the skim equivalent...

  12. Associations between milk protein polymorphisms and milk production traits.

    NARCIS (Netherlands)

    Bovenhuis, H.; Arendonk, van J.A.M.; Korver, S.

    1992-01-01

    Associations between milk protein genotypes and milk production traits were estimated from 6803 first lactation records. Exact tests of associated hypotheses and unbiased estimates of genotype effects were from an animal model. Milk protein genotype effects were estimated using a model in which each

  13. Prion protein in milk.

    Directory of Open Access Journals (Sweden)

    Nicola Franscini

    Full Text Available BACKGROUND: Prions are known to cause transmissible spongiform encephalopathies (TSE after accumulation in the central nervous system. There is increasing evidence that prions are also present in body fluids and that prion infection by blood transmission is possible. The low concentration of the proteinaceous agent in body fluids and its long incubation time complicate epidemiologic analysis and estimation of spreading and thus the risk of human infection. This situation is particularly unsatisfactory for food and pharmaceutical industries, given the lack of sensitive tools for monitoring the infectious agent. METHODOLOGY/PRINCIPAL FINDINGS: We have developed an adsorption matrix, Alicon PrioTrap, which binds with high affinity and specificity to prion proteins. Thus we were able to identify prion protein (PrP(C--the precursor of prions (PrP(Sc--in milk from humans, cows, sheep, and goats. The absolute amount of PrP(C differs between the species (from microg/l range in sheep to ng/l range in human milk. PrP(C is also found in homogenised and pasteurised off-the-shelf milk, and even ultrahigh temperature treatment only partially diminishes endogenous PrP(C concentration. CONCLUSIONS/SIGNIFICANCE: In view of a recent study showing evidence of prion replication occurring in the mammary gland of scrapie infected sheep suffering from mastitis, the appearance of PrP(C in milk implies the possibility that milk of TSE-infected animals serves as source for PrP(Sc.

  14. Breast milk - pumping and storing

    Science.gov (United States)

    ... a comfortable chair, sink, and electric pump. If pumping at work is going to be hard, build ... up your milk supply. Wash your hands before pumping. Collect breast milk when pumping. You can use: ...

  15. Wormholes in Bulk Viscous Cosmology

    OpenAIRE

    Jamil, Mubasher

    2008-01-01

    We investigate the effects of the accretion of phantom energy with non-zero bulk viscosity onto a Morris-Thorne wormhole. We have found that if the bulk viscosity is large then the mass of wormhole increases rapidly as compared to small or zero bulk viscosity.

  16. The influence of milking procedures on cow’s teat tissue, milk yield and milking time

    Directory of Open Access Journals (Sweden)

    Saša Špoljar

    2004-04-01

    Full Text Available The aim of this study was to evaluate the effect of two different milking procedures on teat length and diameter, milk yield and milking time. During the first milking procedure the milker decides when to remove the milking cluster from the udder, while in the second an automatic cluster removal, when milk flow was lower than 200 g/min, was performed. Measurements were made on two commercial farms B and A on 15 randomly chosen cows without any clinical sign of mastitis. There were no significant differencesbetween those farms in teat length and diameter differences before and after the milking. Average milking time on farm A was 6 min and 58 s, while on farm B was 5 min and 3 s. Average milk yield was 12.9 kg on farm B and 13.4 kg on farm A. Milking time was considerably shorter on farm B compared to farm A, while milk yield was almost equal on both farms. Automatic cluster weighting 2 kg removal was performed when milk flow was lower than 200 g/min in order to shorten the time needed to milk the cow, ensuring good udder health.

  17. Alternative Chemical Cleaning Methods for High Level Waste Tanks: Actual Waste Testing with SRS Tank 5F Sludge

    Energy Technology Data Exchange (ETDEWEB)

    King, William D. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Hay, Michael S. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-08-30

    Solubility testing with actual High Level Waste tank sludge has been conducted in order to evaluate several alternative chemical cleaning technologies for the dissolution of sludge residuals remaining in the tanks after the exhaustion of mechanical cleaning and sludge sluicing efforts. Tests were conducted with archived Savannah River Site (SRS) radioactive sludge solids that had been retrieved from Tank 5F in order to determine the effectiveness of an optimized, dilute oxalic/nitric acid cleaning reagent toward dissolving the bulk non-radioactive waste components. Solubility tests were performed by direct sludge contact with the oxalic/nitric acid reagent and with sludge that had been pretreated and acidified with dilute nitric acid. For comparison purposes, separate samples were also contacted with pure, concentrated oxalic acid following current baseline tank chemical cleaning methods. One goal of testing with the optimized reagent was to compare the total amounts of oxalic acid and water required for sludge dissolution using the baseline and optimized cleaning methods. A second objective was to compare the two methods with regard to the dissolution of actinide species known to be drivers for SRS tank closure Performance Assessments (PA). Additionally, solubility tests were conducted with Tank 5 sludge using acidic and caustic permanganate-based methods focused on the “targeted” dissolution of actinide species.

  18. Association of bedding types with management practices and indicators of milk quality on larger Wisconsin dairy farms.

    Science.gov (United States)

    Rowbotham, R F; Ruegg, P L

    2015-11-01

    The objective of this study was to identify associations of bedding type and selected management practices with bulk milk quality and productivity of larger Wisconsin dairy farms. Dairy herds (n=325) producing ≥11,340 kg of milk daily were surveyed during a single farm visit. Monthly bulk milk SCC and total bacteria counts were obtained from milk buyers for 255 farms for a 2-yr period. Of farms with the same type of bedding in all pens during the study period, most used inorganic bedding (IB), followed by organic nonmanure bedding (OB) and manure products (MB). Almost all bulk milk total bacterial counts were subclinical mastitis and 965 kg greater on farms milking 3 times daily. Each 1% increase of cows with nonfunctioning mammary quarters was associated with a decrease of 57 kg of RHA. The BMSCS, proportions of cows with milk discarded and proportion of cows with nonfunctioning mammary quarters were least for herds using IB and were associated with increased productivity. Large Wisconsin dairy farms that used inorganic bedding had greater productivity and better milk quality compared with herds using other bedding types. PMID:26298761

  19. Task 7c: Worm tank

    International Nuclear Information System (INIS)

    Worm tank has a unique shape. In the seismic design of a worm tank, it is desirable to clear the behavior of the worm tank under the seismic loading. We assumed that there are two phenomena in the seismic behavior of the worm tank same as the behavior of the cylindrical and rectangular tanks. One is a sloshing behavior of the water and another is the dynamic response of the worm tank. In this study, we investigate the dynamic characteristics of the worm tank during the strong earthquakes. We conducted the vibration tests to clarify the seismic behaviors of the worm tanks and obtained the valuable data to verify the analytical method. It was found that the natural frequency can be calculated using the eigenvalue formula of the cylindrical and rectangular tanks. Lower modes of the worm tank are identical with that of the rectangular tank. We can estimate the surface behavior and the impact mode using the data of the rectangular tank. (author)

  20. Addition to thermized milk of Lactococcus lactis subsp. cremoris M104, a wild, novel nisin a-producing strain, replaces the natural antilisterial activity of the autochthonous raw milk microbiota reduced by thermization.

    Science.gov (United States)

    Lianou, Alexandra; Samelis, John

    2014-08-01

    Recent research has shown that mild milk thermization treatments routinely used in traditional Greek cheese production are efficient to inactivate Listeria monocytogenes and other pathogenic or undesirable bacteria, but they also inactivate a great part of the autochthonous antagonistic microbiota of raw milk. Therefore, in this study, the antilisterial activity of raw or thermized (63°C, 30 s) milk in the presence or absence of Lactococcus lactis subsp. cremoris M104, a wild, novel, nisin A-producing (Nis-A+) raw milk isolate, was assessed. Bulk milk samples were taken from a local cheese plant before or after thermization and were inoculated with a five-strain cocktail of L. monocytogenes (approximately 4 log CFU/ml) or with the cocktail, as above, plus the Nis-A+ strain (approximately 6 log CFU/ml) as a bioprotective culture. Heat-sterilized (121°C, 5 min) raw milk inoculated with L. monocytogenes was used as a control treatment. All milk samples were incubated at 37°C for 6 h and then at 18°C for an additional 66 h. L. monocytogenes grew abundantly (>8 log CFU/ml) in heat-sterilized milk, whereas its growth was completely inhibited in all raw milk samples. Conversely, in thermized milk, L. monocytogenes increased by 2 log CFU/ml in the absence of strain M104, whereas its growth was completely inhibited in the presence of strain M104. Furthermore, nisin activity was detected only in milk samples inoculated with strain M104. Thus, postthermal supplementation of thermized bulk milk with bioprotective L. lactis subsp. cremoris cultures replaces the natural antilisterial activity of raw milk reduced by thermization.

  1. ANALYSIS OF THE TANK 5F FINAL CHARATERIZATION SAMPLES-2011

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L.; Diprete, D.; Coleman, C.; Hay, M.

    2012-01-20

    The Savannah River National Laboratory (SRNL) was requested by SRR to provide sample preparation and analysis of the Tank 5F final characterization samples to determine the residual tank inventory prior to grouting. Two types of samples were collected and delivered to SRNL: floor samples across the tank and subsurface samples from mounds near risers 1 and 5 of Tank 5F. These samples were taken from Tank 5F between January and March 2011. These samples from individual locations in the tank (nine floor samples and six mound Tank 5F samples) were each homogenized and combined in a given proportion into 3 distinct composite samples to mimic the average composition in the entire tank. These Tank 5F composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 5F composite samples include bulk density and water leaching of the solids to account for water soluble species. With analyses for certain challenging radionuclides as the exception, all composite Tank 5F samples were analyzed and reported in triplicate. The target detection limits for isotopes analyzed were based on customer desired detection limits as specified in the technical task request documents. SRNL developed new methodologies to meet these target detection limits and provide data for the extensive suite of components. While many of the target detection limits were met for the species characterized for Tank 5F, as specified in the technical task request, some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The Technical Task Request allows that while the analyses of these isotopes is needed, meeting the detection limits for these isotopes is a lower priority than meeting detection limits for the other specified isotopes. The isotopes whose detection limits were not met in all cases included the

  2. Analysis Of The Tank 5F Final Characterization Samples-2011

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L. N.; Diprete, D.; Coleman, C. J.; Hay, M. S.

    2012-09-27

    The Savannah River National Laboratory (SRNL) was requested by SRR to provide sample preparation and analysis of the Tank 5F final characterization samples to determine the residual tank inventory prior to grouting. Two types of samples were collected and delivered to SRNL: floor samples across the tank and subsurface samples from mounds near risers 1 and 5 of Tank 5F. These samples were taken from Tank 5F between January and March 2011. These samples from individual locations in the tank (nine floor samples and six mound Tank 5F samples) were each homogenized and combined in a given proportion into 3 distinct composite samples to mimic the average composition in the entire tank. These Tank 5F composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 5F composite samples include bulk density and water leaching of the solids to account for water soluble species. With analyses for certain challenging radionuclides as the exception, all composite Tank 5F samples were analyzed and reported in triplicate. The target detection limits for isotopes analyzed were based on customer desired detection limits as specified in the technical task request documents. SRNL developed new methodologies to meet these target detection limits and provide data for the extensive suite of components. While many of the target detection limits were met for the species characterized for Tank 5F, as specified in the technical task request, some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The Technical Task Request allows that while the analyses of these isotopes is needed, meeting the detection limits for these isotopes is a lower priority than meeting detection limits for the other specified isotopes. The isotopes whose detection limits were not met in all cases included the

  3. ANALYSIS OF THE TANK 5F FINAL CHARACTERIZATION SAMPLES-2011

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L.; Diprete, D.; Coleman, C.; Hay, M.

    2012-08-03

    The Savannah River National Laboratory (SRNL) was requested by SRR to provide sample preparation and analysis of the Tank 5F final characterization samples to determine the residual tank inventory prior to grouting. Two types of samples were collected and delivered to SRNL: floor samples across the tank and subsurface samples from mounds near risers 1 and 5 of Tank 5F. These samples were taken from Tank 5F between January and March 2011. These samples from individual locations in the tank (nine floor samples and six mound Tank 5F samples) were each homogenized and combined in a given proportion into 3 distinct composite samples to mimic the average composition in the entire tank. These Tank 5F composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 5F composite samples include bulk density and water leaching of the solids to account for water soluble species. With analyses for certain challenging radionuclides as the exception, all composite Tank 5F samples were analyzed and reported in triplicate. The target detection limits for isotopes analyzed were based on customer desired detection limits as specified in the technical task request documents. SRNL developed new methodologies to meet these target detection limits and provide data for the extensive suite of components. While many of the target detection limits were met for the species characterized for Tank 5F, as specified in the technical task request, some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The Technical Task Request allows that while the analyses of these isotopes is needed, meeting the detection limits for these isotopes is a lower priority than meeting detection limits for the other specified isotopes. The isotopes whose detection limits were not met in all cases included the

  4. Milking procedures, milk flow curves and somatic cell count in dairy cows

    Directory of Open Access Journals (Sweden)

    L. Zanini

    2010-01-01

    Full Text Available Recent availability of electronic mobile milk flow meters allows to study in details milk flow patterns during milking. A normal milk flow profile is characterized by an incline phase, with increasing milk flow, a plateau phase, with steady milk flow, and a decline phase. In these phases milk flow is influenced by many factors: genetic characteristics of the cows, regulation of milking machine and milking routine.

  5. [Breast milk substitutes based on cow milk].

    Science.gov (United States)

    Kofoed, P E

    1990-08-20

    "Adapted" or "humanized" breast-milk substitutes based on cows' milk are manufactured according to directives from a publication issued by the Ministry of Agriculture. The accepted recommendations for the daily intake (RDA) of nutrients is adjusted to the neonates' relatively low tolerance and provides a certain margin of safety in case of illness and slight inaccuracies in preparation. The recommendations are, however, often based on animal experiments, studies of pathological conditions etc. because the needs of the neonate are not known. There is a fundamental difference between RDA for chemical energy and various nutrients as the energy requirement is stated on the basis of average values while the requirements for specific nutrients are gives as upper and lower limiting values. In addition to nutrients, a long series of hormones, enzymes and antimicrobial factors are transferred to the infant via breast-milk. The nutritional significance of these is entirely or partially unknown. It is thus impossible to give the bottle-fed infant a diet which is quantitatively and qualitatively identical with that of a breastfed baby. Nevertheless, experience has shown that bottle-feeding usually proceeds satisfactorily. Galactosaemia and certain forms of medication in the mother constitute absolute contraindications to breast-feeding while phenylketonuria, certain maternal infections are relative contraindications to breast-feeding. Mothers should be prepared for breast-feeding already during pregnancy but in the cases where the mother cannot, should not or does not wish to breast-feed, it is important to counteract any feelings of guilt, neglect or incompetence and, on the other hand, give her thorough training in artificial feeding of the infant. PMID:2205958

  6. Scanning Fuel Tanks’ Corrosion Wastage of Some Aged Bulk Carriers Due to Security Reasons

    OpenAIRE

    Bauk, Sanja; Aleksić, Marinko; Ivošević, Špiro

    2011-01-01

    This paper deals with two different approaches in modelling corrosion wastage over the fuel tanks’ structures on the example of ten aged bulk carriers. The first applied method might be treated as a short-term, rather random oriented one, and it is based on the Monte Carlo simulation technique. This technique has been used in creating an appropriate predictive model for the characteristic steel damages over the bulk carriers’ fuel tanks caused by general corrosion in relatively short time int...

  7. Raw milk consumption and health

    OpenAIRE

    Popović-Vranješ Anka; Popović Milka; Jevtić Marija

    2015-01-01

    Contrary to the safe practices of milk pasteurization or sterilization, which effectively reduce foodborne outbreaks incidence associated with raw milk and dairy products use, outbreaks caused by such products continue to occur. Despite this fact, a worldwide movement advocating for the rights of raw milk and cheese selling and consumption, due to their specific nutritive characteristics, has strengthened significantly in recent years. Traditional agricultu...

  8. Yoghurt fermentation trials utilizing mare milk: comparison with cow milk

    Directory of Open Access Journals (Sweden)

    R. Giangiacomo

    2011-03-01

    Full Text Available Mare milk shows a very interesting nutritional composition, similar to human milk. Whey protein fraction represents about 50% of total proteins, with a good amount of essential amino acids, and high lysozyme concentration (Jauregui-Adell, 1975. Mare milk contains essential fatty acids, progenitors of ω3 and ω6, higher than cow milk (Csapò et al., 1995; Curadi et al., 2002. In east european countries mare milk is utilized in dietetics and therapeutics for gastroenteric and cardiac pathologies (Sharmanov et al., 1982; Mirrakimov et al., 1986, or as a drink obtained from lactic and alcoholic fermentation (Koumiss...

  9. Milk Production in Tibet

    Institute of Scientific and Technical Information of China (English)

    Qiumei Ji; Tsam You; Zhang Oiang

    2008-01-01

    This paper describes milk production and livestock production in Tibet.Some information of market demand has also been presented.There has been very little information published in Tibetan journals on production and nutrition of cattle.This review provides a brief introduction to feeding systems and feeding resources.Many studies on milk production have been done in isolation,and do not go beyond the basic and practical level.Compared with dairy cattle research in other parts of China,large gaps in knowledge still exist in cattle production science,particularly related to nutrition,and systems approaches for the development of a dairy industry.

  10. Changes in the amino acid composition of buffalo milk after chemical activation of its lactoperoxidase system

    Directory of Open Access Journals (Sweden)

    M. Tsankova

    2010-02-01

    Full Text Available The amino acid content of bulked buffalo milk, collected from 130 buffaloes reared at a buffalo farm in the settlement of Dimitrievo, Stara Zagora region, was investigated during the period January-April 2006. The activation of the lactoperoxidase system (LPS was done by supplementation of sodium percarbonate, providing 16 ppm active oxygen and 10 ppm thiocyanate to 1 l of milk. The amino acid content was assayed by an amino acid analyzer. It was found out that the total content of amino acids in inactivated milk was insignificantly lower than that in activated one. More considerable increase was established for the amino acids valine, methionine, and lysine, but the differences were not statistically significant. The total amount of essential amino acids was higher in the milk with chemically activated LPS. The limiting essential amino acid in the studied buffalo milk was methionine.

  11. The Incredible Bulk

    CERN Document Server

    Fukushima, Keita; Kumar, Jason; Sandick, Pearl; Yamamoto, Takahiro

    2014-01-01

    Recent experimental results from the LHC have placed strong constraints on the masses of colored superpartners. The MSSM parameter space is also constrained by the measurement of the Higgs boson mass, and the requirement that the relic density of lightest neutralinos be consistent with observations. Although large regions of the MSSM parameter space can be excluded by these combined bounds, leptophilic versions of the MSSM can survive these constraints. In this paper we consider a scenario in which the requirements of minimal flavor violation, vanishing $CP$-violation, and mass universality are relaxed, specifically focusing on scenarios with light sleptons. We find a large region of parameter space, analogous to the original bulk region, for which the lightest neutralino is a thermal relic with an abundance consistent with that of dark matter. We find that these leptophilic models are constrained by measurements of the magnetic and electric dipole moments of the electron and muon, and that these models have ...

  12. Creating bulk nanocrystalline metal.

    Energy Technology Data Exchange (ETDEWEB)

    Fredenburg, D. Anthony (Georgia Institute of Technology, Atlanta, GA); Saldana, Christopher J. (Purdue University, West Lafayette, IN); Gill, David D.; Hall, Aaron Christopher; Roemer, Timothy John (Ktech Corporation, Albuquerque, NM); Vogler, Tracy John; Yang, Pin

    2008-10-01

    Nanocrystalline and nanostructured materials offer unique microstructure-dependent properties that are superior to coarse-grained materials. These materials have been shown to have very high hardness, strength, and wear resistance. However, most current methods of producing nanostructured materials in weapons-relevant materials create powdered metal that must be consolidated into bulk form to be useful. Conventional consolidation methods are not appropriate due to the need to maintain the nanocrystalline structure. This research investigated new ways of creating nanocrystalline material, new methods of consolidating nanocrystalline material, and an analysis of these different methods of creation and consolidation to evaluate their applicability to mesoscale weapons applications where part features are often under 100 {micro}m wide and the material's microstructure must be very small to give homogeneous properties across the feature.

  13. Diffusion or bulk flow

    DEFF Research Database (Denmark)

    Schulz, Alexander

    2015-01-01

    the concentration gradient or bulk flow along a pressure gradient. The driving force seems to depend on the mode of phloem loading. In a majority of plant species phloem loading is a thermodynamically active process, involving the activity of membrane transporters in the sieve-element companion cell complex. Since...... assimilate movement includes an apoplasmic step, this mode is called apoplasmic loading. Well established is also the polymer-trap loading mode, where the phloem-transport sugars are raffinose-family oligomers in herbaceous plants. Also this mode depends on the investment of energy, here for sugar......Assimilates synthesized in the mesophyll of mature leaves move along the pre-phloem transport pathway to the bundle sheath of the minor veins from which they are loaded into the phloem. The present review discusses the most probable driving force(s) for the pre-phloem pathway, diffusion down...

  14. Bulk-Fill Resin Composites

    DEFF Research Database (Denmark)

    Benetti, Ana Raquel; Havndrup-Pedersen, Cæcilie; Honoré, Daniel;

    2015-01-01

    the restorative procedure. The aim of this study, therefore, was to compare the depth of cure, polymerization contraction, and gap formation in bulk-fill resin composites with those of a conventional resin composite. To achieve this, the depth of cure was assessed in accordance with the International Organization...... for Standardization 4049 standard, and the polymerization contraction was determined using the bonded-disc method. The gap formation was measured at the dentin margin of Class II cavities. Five bulk-fill resin composites were investigated: two high-viscosity (Tetric EvoCeram Bulk Fill, SonicFill) and three low......-viscosity (x-tra base, Venus Bulk Fill, SDR) materials. Compared with the conventional resin composite, the high-viscosity bulk-fill materials exhibited only a small increase (but significant for Tetric EvoCeram Bulk Fill) in depth of cure and polymerization contraction, whereas the low-viscosity bulk...

  15. Tank characterization technical sampling basis

    Energy Technology Data Exchange (ETDEWEB)

    Brown, T.M.

    1998-04-28

    Tank Characterization Technical Sampling Basis (this document) is the first step of an in place working process to plan characterization activities in an optimal manner. This document will be used to develop the revision of the Waste Information Requirements Document (WIRD) (Winkelman et al. 1997) and ultimately, to create sampling schedules. The revised WIRD will define all Characterization Project activities over the course of subsequent fiscal years 1999 through 2002. This document establishes priorities for sampling and characterization activities conducted under the Tank Waste Remediation System (TWRS) Tank Waste Characterization Project. The Tank Waste Characterization Project is designed to provide all TWRS programs with information describing the physical, chemical, and radiological properties of the contents of waste storage tanks at the Hanford Site. These tanks contain radioactive waste generated from the production of nuclear weapons materials at the Hanford Site. The waste composition varies from tank to tank because of the large number of chemical processes that were used when producing nuclear weapons materials over the years and because the wastes were mixed during efforts to better use tank storage space. The Tank Waste Characterization Project mission is to provide information and waste sample material necessary for TWRS to define and maintain safe interim storage and to process waste fractions into stable forms for ultimate disposal. This document integrates the information needed to address safety issues, regulatory requirements, and retrieval, treatment, and immobilization requirements. Characterization sampling to support tank farm operational needs is also discussed.

  16. North Tank Farm data report for the Gunite and Associated Tanks at Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Rule, V.A. [XL Associates, Inc., Oak Ridge, TN (United States); Burks, B.L. [Providence Group, Knoxville, TN (United States); Hoesen, S.D. van [Oak Ridge National Lab., TN (United States)

    1998-05-01

    The US Department of Energy (DOE) Office of Science and Technology, in cooperation with the Oak Ridge Environmental Management Program, has developed and demonstrated the first full-scale remotely operated system for cleaning radioactive liquid and waste from large underground storage tanks. The remotely operated waste retrieval system developed and demonstrated at Oak Ridge National Laboratory (ORNL) is designed to accomplish both retrieval of bulk waste, including liquids, thick sludge, and scarified concrete, and final tank cleaning. This report provides a summary of the North Tank Farm (NTF) operations data and an assessment of the performance and efficiency of the waste retrieval system during NTF operations data and an assessment of the performance and efficiency of the waste retrieval system during NTF operations. The organization of this report is as follows: Section 1 provides an introduction to the report. Section 2 describes the NTF tank structures (W-3 and W-4 only) and the contents of the tanks. Section 3 outlines the objectives of the NTF testing and explains how these objectives were met. Section 4 provides a description of the various operating systems used in the NTF operations. Sections 5 and 6 present a summary of the data collected during NTF operations. Section 7 summarizes the maintenance activities performed and Section 8 summarizes the on-the-job training performed in the NTF. Section 9 summarizes the capital cost for the waste retrieval and characterization equipment and operating costs for performing the NTF work. Section 10 provides observations and lessons learned, and Section 11 provides a summary and conclusions.

  17. Do Fish Enhance Tank Mixing?

    DEFF Research Database (Denmark)

    Rasmussen, Michael R.; Laursen, Jesper; Craig, Steven R.;

    2005-01-01

    The design of fish rearing tanks represents a critical stage in the development of optimal aquaculture systems, especially in the context of recirculating systems. Poor hydrodynamics can compromise water quality, waste management and the physiology and behaviour of fish, and thence, production...... potential and operational profitability. The hydrodynamic performance of tanks, therefore, represents an important parameter during the tank design process. Because there are significant complexities in combining the rigid principles of hydrodynamics with the stochastic behaviour of fish, however, most data...... upon tank hydrokinetics has been derived using tanks void of fish. Clearly, the presence of randomly moving objects, such as fish, in a water column will influence not only tank volumes by displacing water, but due to their activity, water dynamics and associated in-tank processes. In order...

  18. NMR-based milk metabolomics

    DEFF Research Database (Denmark)

    Sundekilde, Ulrik; Larsen, Lotte Bach; Bertram, Hanne Christine S.

    2013-01-01

    Milk is a key component in infant nutrition worldwide and, in the Western parts of the world, also in adult nutrition. Milk of bovine origin is both consumed fresh and processed into a variety of dairy products including cheese, fermented milk products, and infant formula. The nutritional quality...... compounds. Furthermore, metabolomics applications elucidating how the differential regulated genes affects milk composition are also reported. This review will highlight the recent advances in NMR-based metabolomics on milk, as well as give a brief summary of when NMR spectroscopy can be useful for gaining...

  19. Precipitation and Deposition of Aluminum-Containing Phases in Tank Wastes

    International Nuclear Information System (INIS)

    Aluminum-containing phases compose the bulk of solids precipitating during the processing of radioactive tank wastes. Processes designed to minimize the volume of high-level waste through conversion to glassy phases require transporting waste solutions near-saturated with aluminum-containing species from holding tank to processing center. The uncontrolled precipitation within transfer lines results in clogged pipes and lines and fouled ion exchangers, with the potential to shut down processing operations

  20. Precipitation and Deposition of Aluminum-Containing Phases in Tank Wastes

    Energy Technology Data Exchange (ETDEWEB)

    Daniel M. Dabbs; Ilhan A. Aksay

    2005-01-12

    Aluminum-containing phases compose the bulk of solids precipitating during the processing of radioactive tank wastes. Processes designed to minimize the volume of high-level waste through conversion to glassy phases require transporting waste solutions near-saturated with aluminum-containing species from holding tank to processing center. The uncontrolled precipitation within transfer lines results in clogged pipes and lines and fouled ion exchangers, with the potential to shut down processing operations.

  1. Contaminants in human milk.

    Science.gov (United States)

    Olszyna-Marzys, A E

    1978-09-01

    There is a paucity of information regarding excretion of contaminants in human milk, due to experimental difficulties and until recently a general lack of interest. Because of the high fat content of milk and as its acidity is higher than that of plasma, nearly all liposoluble and basic agents consumed by the mother will be excreted in the milk. Distinction must be made between, on the one hand drugs and social toxicants such as smoking and alcohol, whose intake can be stopped or limited during pregnancy and lactation, and ecological toxicants present in a polluted environment to which the mother is exposed. Cases have occurred of heavy prenatal and postnatal intoxication of infants with hexachlorobenzene in Turkey and methylmercury in Iraq due to consumption of fungicide-treated seed wheat by pregnant and lactating mothers. Recent attention has been concentrated on contamination of milk with organochlorine compounds such as DDT and PCB's, that are found in many parts of the world. The heaviest contamination with DDT has been found in Guatemala, resulting in suckling infants consuming many times the Acceptable Daily Intake of this compound proposed by WHO, with unknown future effects.

  2. The Sweetest Chocolate Milk

    Science.gov (United States)

    Newton, Kristie J.

    2010-01-01

    Using a nonroutine problem can be an effective way to encourage students to draw on prior knowledge, work together, and reach important conclusions about the mathematics they are learning. This article discusses a problem on the mathematical preparation of chocolate milk which was adapted from an old book of puzzles (Linn 1969) and has been used…

  3. A Sour Milk Rivalry

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A libel scandal involving Mengniu taints its image and deals a blow to China’s dairy industry Mengniu,with the 2008 milk contamination incident still fresh in people’s memories,has brought China’s dairy industry into the spotlight again-this time the attention focused on a bitter rivalry.

  4. Bovine milk exosome proteome

    Science.gov (United States)

    Exosomes are 40-100 nm membrane vesicles of endocytic origin and are found in blood, urine, amniotic fluid, bronchoalveolar lavage (BAL) fluid, as well as human and bovine milk. Exosomes are extracellular organelles important in intracellular communication/signaling, immune function, and biomarkers ...

  5. Residual contaminants in milk

    Directory of Open Access Journals (Sweden)

    Nevijo Zdolec

    2006-07-01

    Full Text Available Various chemical agents are used during the whole production chain of milk and dairy products. Production of feedingstuffs is accompanied with pesticide usage, which may remain in environment, thus are transported through feeding into animals, animal products and finally in human organism. Preparation procedure and storage conditions of feed also influence on milk safety in the sense of mycotoxins entering into the food chain. Chemical agents are, on daily basis, used on dairy farms either as detergents or disinfections. The residuals of cleaning agents might remain in milk if the cleaning agents and its dosage are not performed adequately. Besides already mentioned agents, a great influence in milk production can bee seen through veterinary drugs usage, particularly antibacterial drugs (mastitis. Proper application of drugs and by following legal recommendation, a by-reactions can be avoided such as allergic reaction in humans, development of resisting bacteria or even undesirable influence on starter cultures in dairy products manufacture. The maximum residue limits, monitoring plan as well as sampling procedures are set up within the harmonization of Croatian and European legislation, in order to provide official control of residues in foodstuffs of animal origin.

  6. Tank characterization data report: Tank 241-C-112

    International Nuclear Information System (INIS)

    Tank 241-C-112 is a Hanford Site Ferrocyanide Watch List tank that was most recently sampled in March 1992. Analyses of materials obtained from tank 241-C-112 were conducted to support the resolution of the Ferrocyanide Unreviewed Safety Question (USQ) and to support Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) Milestone M-10-00. Analysis of core samples obtained from tank 241-C-112 strongly indicates that the fuel concentration in the tank waste will not support a propagating exothermic reaction. It is probable that tank 241-C-112 exceeds the 1,000 g-mol inventory criteria established for the Ferrocyanide USQ; however, extensive energetic analysis of the waste has determined a maximum exothermic value of -9 cal/g dry waste. This value is substantially below any levels of concern (-75 cal/g). In addition, an investigation of potential mechanisms to generate concentration levels of radionuclides high enough to be of concern was performed. No credible mechanism was postulated that could initiate the formation of such concentration levels in the tank. Tank 241-C-112 waste is a complex material made up primarily of water and inert salts. The insoluble solids are a mixture of phosphates, sulfates, and hydroxides in combination with aluminum, calcium, iron, nickel, and uranium. Disodium nickel ferrocyanide and sodium cesium nickel ferrocyanide probably exist in the tank; however, there appears to have been significant degradation of this material since the waste was initially settled in the tank

  7. 27 CFR 25.145 - Tanks, vehicles, and vessels.

    Science.gov (United States)

    2010-04-01

    ... BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Marks, Brands, and Labels § 25.145 Tanks, vehicles, and... mark each tank, tank car, tank truck, tank ship, barge, or deep tank of a vessel in accordance...

  8. Analysis of the Tank 6F Final Characterization Samples-2012

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L. N.; Diprete, D. P.; Coleman, C. J.; Hay, M. S.; Shine, E. P.

    2013-01-31

    The Savannah River National Laboratory (SRNL) was requested by Savannah River Remediation (SRR) to provide sample preparation and analysis of the Tank 6F final characterization samples to determine the residual tank inventory prior to grouting. Fourteen residual Tank 6F solid samples from three areas on the floor of the tank were collected and delivered to SRNL between May and August 2011. These Tank 6F samples were homogenized and combined into three composite samples based on a proportion compositing scheme and the resulting composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 6F composite samples include bulk density and water leaching of the solids to account for water soluble components. The composite Tank 6F samples were analyzed and the data reported in triplicate. Sufficient quality assurance standards and blanks were utilized to demonstrate adequate characterization of the Tank 6F samples. The main evaluation criteria were target detection limits specified in the technical task request document. While many of the target detection limits were met for the species characterized for Tank 6F some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The isotopes whose detection limits were not met in all cases included Sn-126, Sb-126, Sb-126m, Eu-152, Cm- 243 and Cf-249. SRNL, in conjunction with the customer, reviewed all of these cases and determined that the impacts of not meeting the target detection limits were acceptable. Based on the analyses of variance (ANOVA) for the inorganic constituents of Tank 6F, all the inorganic constituents displayed heterogeneity. The inorganic results demonstrated consistent differences across the composite samples: lowest concentrations for Composite Sample 1, intermediate-valued concentrations for Composite

  9. Analysis Of The Tank 6F Final Characterization Samples-2012

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L. N.; Diprete, D. P.; Coleman, C. J.; Hay, M. S.; Shine, E. P.

    2012-09-27

    The Savannah River National Laboratory (SRNL) was requested by Savannah River Remediation (SRR) to provide sample preparation and analysis of the Tank 6F final characterization samples to determine the residual tank inventory prior to grouting. Fourteen residual Tank 6F solid samples from three areas on the floor of the tank were collected and delivered to SRNL between May and August 2011. These Tank 6F samples were homogenized and combined into three composite samples based on a proportion compositing scheme and the resulting composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 6F composite samples include bulk density and water leaching of the solids to account for water soluble components. The composite Tank 6F samples were analyzed and the data reported in triplicate. Sufficient quality assurance standards and blanks were utilized to demonstrate adequate characterization of the Tank 6F samples. The main evaluation criteria were target detection limits specified in the technical task request document. While many of the target detection limits were met for the species characterized for Tank 6F some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The isotopes whose detection limits were not met in all cases included Sn-126, Sb-126, Sb-126m, Eu-152, Cm-243 and Cf-249. SRNL, in conjunction with the customer, reviewed all of these cases and determined that the impacts of not meeting the target detection limits were acceptable. Based on the analyses of variance (ANOVA) for the inorganic constituents of Tank 6F, all the inorganic constituents displayed heterogeneity. The inorganic results demonstrated consistent differences across the composite samples: lowest concentrations for Composite Sample 1, intermediate-valued concentrations for Composite

  10. ANALYSIS OF THE TANK 6F FINAL CHARACTERIZATION SAMPLES-2012

    Energy Technology Data Exchange (ETDEWEB)

    Oji, L.; Diprete, D.; Coleman, C.; Hay, M.; Shine, G.

    2012-06-28

    The Savannah River National Laboratory (SRNL) was requested by Savannah River Remediation (SRR) to provide sample preparation and analysis of the Tank 6F final characterization samples to determine the residual tank inventory prior to grouting. Fourteen residual Tank 6F solid samples from three areas on the floor of the tank were collected and delivered to SRNL between May and August 2011. These Tank 6F samples were homogenized and combined into three composite samples based on a proportion compositing scheme and the resulting composite samples were analyzed for radiological, chemical and elemental components. Additional measurements performed on the Tank 6F composite samples include bulk density and water leaching of the solids to account for water soluble components. The composite Tank 6F samples were analyzed and the data reported in triplicate. Sufficient quality assurance standards and blanks were utilized to demonstrate adequate characterization of the Tank 6F samples. The main evaluation criteria were target detection limits specified in the technical task request document. While many of the target detection limits were met for the species characterized for Tank 6F some were not met. In a few cases, the relatively high levels of radioactive species of the same element or a chemically similar element precluded the ability to measure some isotopes to low levels. The isotopes whose detection limits were not met in all cases included Sn-126, Sb-126, Sb-126m, Eu-152, Cm-243 and Cf-249. SRNL, in conjunction with the customer, reviewed all of these cases and determined that the impacts of not meeting the target detection limits were acceptable. Based on the analyses of variance (ANOVA) for the inorganic constituents of Tank 6F, all the inorganic constituents displayed heterogeneity. The inorganic results demonstrated consistent differences across the composite samples: lowest concentrations for Composite Sample 1, intermediate-valued concentrations for Composite

  11. Diagnóstico de brucelosis bovina en leche por el Ring Test y ELISA en lecherías de la provincia de Ñuble (VIII Región Bovine brucellosis diagnosis in milk by Ring Test and ELISA in dairy farms of Ñuble (VIII Region, Chile

    Directory of Open Access Journals (Sweden)

    J. LOPEZ

    1998-01-01

    Full Text Available Se utilizaron los métodos de ELISA y Ring Test en leche para diagnosticar brucelosis bovina en estanques de recolección en frío a 60 predios lecheros de la provincia de Ñuble, VIII Región de Chile, durante los meses de noviembre y diciembre de 1995. Estos fueron estratificados en relación al número de vacas en producción de leche. Los resultados obtenidos por ambos métodos fueron similares,18 muestras positivas y 42 negativas. La diferencia se estableció en 5 muestras (8.3% que fueron clasificadas como positivas débiles al Ring Test y confirmadas como positivas por ELISA. Se determinó que la prevalencia predial de brucelosis bovina fue de un 30% en predios lecheros con estanque enfriador en la provincia de Ñuble. El métodode ELISA resultó ser una prueba confiable para diagnosticar brucelosis bovina en leches colectivas.ELISA and Ring Test were compared as diagnostic tests for bovine brucellosis in bulk tank refrigerated milk from 60 dairies in the Ñuble Province, VIII Region, Chile, from November to December. Samples were stratified according to the number of cows in the farm. Diagnosis obtained from both methods gave similar results: 18 positive and 42 negative. The difference was established in 5 samples (8.3% that were classified as weak positive to the Ring Test and confirmed as positives by ELISA. Furthermore, it was determined that there was 30% prevalence of bovine brucellosis in dairies with refrigerated tanks in the Ñuble province. The ELISA proved to be a reliable diagnostic test of bovine brucellosis in bulk milk

  12. Camel Milk Is a Safer Choice than Goat Milk for Feeding Children with Cow Milk Allergy

    OpenAIRE

    Mohammad Ehlayel; Abdulbari Bener; Khalid Abu Hazeima; Fatima Al-Mesaifri

    2011-01-01

    Background. Various sources of mammalian milk have been tried in CMA. Objectives. To determine whether camel milk is safer than goat milk in CMA. Methods. Prospective study conducted at Hamad Medical Corporation between April 2007 and April 2010, on children with CMA. Each child had medical examination, CBC, total IgE, cow milk-specific IgE and SPT. CMA children were tested against fresh camel and goat milks. Results. Of 38 children (median age 21.5 months), 21 (55.3%) presented with urticari...

  13. Theoretical study of solar combisystems based on bikini tanks and tank-in-tank stores

    DEFF Research Database (Denmark)

    Yazdanshenas, Eshagh; Furbo, Simon

    2012-01-01

    Purpose - Low flow bikini solar combisystems and high flow tank-in-tank solar combisystems have been studied theoretically. The aim of the paper is to study which of these two solar combisystem designs is suitable for different houses. The thermal performance of solar combisystems based on the two...... different heat storage types is compared. Design/methodology/approach - The thermal performance of Low flow bikini solar combisystems and high flow tank-in-tank solar combisystems is calculated with the simulation program TRNSYS. Two different TRNSYS models based on measurements were developed and used....... Findings - Based on the calculations it is concluded that low flow solar combisystems based on bikini tanks are promising for low energy buildings, while solar combisystems based on tank-in-tank stores are attractive for the houses with medium heating demand and old houses with high heating demand...

  14. Associations between milk protein polymorphisms and milk production traits.

    Science.gov (United States)

    Bovenhuis, H; Van Arendonk, J A; Korver, S

    1992-09-01

    Associations between milk protein genotypes and milk production traits were estimated from 6803 first lactation records. Exact tests of associated hypotheses and unbiased estimates of genotype effects were from an animal model. Milk protein genotype effects were estimated using a model in which each milk protein gene was analyzed separately (single-gene analysis) and a model in which all milk protein genes were analyzed simultaneously (multigene analysis). The results of the two models indicate that some effects ascribed to certain milk protein genes in the single-gene analysis are not effects of the milk protein gene itself but of linked genes. Results from this study and from literature indicate that the kappa-casein gene or a very closely linked gene affects protein percentage, and the beta-lactoglobulin gene or a very closely linked gene affects fat percentage. Furthermore, effects of beta-casein genotypes on milk production, fat percentage, and protein yield were significant, and beta-lactoglobulin genotypes had significant effects on milk production and protein yield. It is less clear whether those effects are due to effects of milk protein genes themselves or to effects of linked genes.

  15. A science think tank

    International Nuclear Information System (INIS)

    A journalist views on public perceptions on nuclear issues in Australia and Japan is presented. It is also emphasised that by not offering an undergraduate course in nuclear engineering, Australia have closed the door to the nuclear energy development in Australia and costed the country some depth of specialized knowledges. A scientific think tank with active participation of the nuclear scientists is thought to benefit Australia and be in the position to influence private industrial and governmental planning

  16. The effect of prestimulation on milking characteristics during machine milking of goat

    Directory of Open Access Journals (Sweden)

    Zoran Bašić

    2009-06-01

    Full Text Available Goat milking is optimal if maximum milk yield is obtained in the minimum time frame. Prestimulation influences milk yield and other milking characteristics. The aim of this study was to determine optimal milking characteristics for different times spent on prestimulation during machine milking of goats. Prestimulation of 30 s was optimal to achieve maximum milk yield and flow in the shortest time. Longer milking interval resulted in higher milk yield, milk flow, but also in the longest milking time. Milking goats in 4th parity had higher average and peak flow rate, while goats in third parity had longer milking time, with higher milk yield. The results of this study show that prestimulation time longer than 30 s is not necessary to obtain optimum milking characteristics during machine milking of goats.

  17. Prevalencia y distribución espacial de brucelosis, leucosis bovina, diarrea viral bovina y rinotraqueítis infecciosa bovina a partir del análisis ELISA de estanques prediales en lecherías de la IX Región, Chile Prevalence and space distribution of brucellosis, bovine leukaemia, bovine viral diarrhea and infectious bovine rhinotracheitis by using bulk milk ELISA test in dairy herds of the IX Region, Chile

    Directory of Open Access Journals (Sweden)

    R Felmer

    2009-01-01

    of the OIE List, including foot and mouth disease and classical swine fever. However, several infectious diseases are known to remain among herds, which produce a major effect in production due to losses by abortion, decrease of fertility and what it is more important, some of them represent barriers for export and constitute a risk of zoonosis for the population. In this work, a monitoring system based on the analysis of bulk milk antibodies by means of ELISA test, was implemented to study the epidemiology and distribution of 4 of the main bovine diseases that currently affect the IX Region of Chile (brucellosis, bovine leukaemia, IBR and BVD. The system allowed the surveillance of 279 dairies, which represented 43% of the dairies registered in IX the Region, and included 19,635 milking cows (14%. With this system, a high prevalence for leukaemia (59%, IBR (76% and BVD (96% could be established, whereas it was confirmed that brucellosis is restricted to a few dairies (5%. The surveillance system coupled to the satellite geographic information analysis, allowed to establish the space distribution of these diseases in the different communes of the Region, demonstrating to be an excellent and low cost support tool for the monitoring of the diseases in the herd, which guarantees the possibility of establishing this platform in the Region and its feasibility to project it at national level.

  18. Calcination/dissolution testing for Hanford Site tank wastes

    Energy Technology Data Exchange (ETDEWEB)

    Colby, S.A.; Delegard, C.H. [Westinghouse Hanford Co., Richland, WA (United States); McLaughlin, D.F. [Westinghouse Science and Technology Center, Pittsburgh, PA (United States); Danielson, M.J. [Pacific Northwest Lab., Richland, WA (United States)

    1994-07-01

    Thermal treatment by calcination offers several benefits for the treatment of Hanford Site tank wastes, including the destruction of organics and ferrocyanides and an hydroxide fusion that permits the bulk of the mostly soluble nonradioactive constituents to be easily separated from the insoluble transuranic residue. Critical design parameters were tested, including: (1) calciner equipment design, (2) hydroxide fusion chemistry, and (3) equipment corrosion. A 2 gal/minute pilot plant processed a simulated Tank 101-SY waste and produced a free flowing 700 C molten calcine with an average calciner retention time of 20 minutes and >95% organic, nitrate, and nitrite destruction. Laboratory experiments using actual radioactive tank waste and the simulated waste pilot experiments indicate that 98 wt% of the calcine produced is soluble in water, leaving an insoluble transuranic fraction. All of the Hanford Site tank wastes can benefit from calcination/dissolution processing, contingent upon blending various tank waste types to ensure a target of 70 wt% sodium hydroxide/nitrate/nitrite fluxing agent. Finally, corrosion testing indicates that a jacketed nickel liner cooled to below 400 C would corrode <2 mil/year (0.05 mm/year) from molten calcine attack.

  19. Tank characterization data report: Tank 241-C-112

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, B.C.; Borsheim, G.L.; Jensen, L.

    1993-09-01

    Tank 241-C-112 is a Hanford Site Ferrocyanide Watch List tank that was most recently sampled in March 1992. Analyses of materials obtained from tank 241-C-112 were conducted to support the resolution of the Ferrocyanide Unreviewed Safety Question (USQ) and to support Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) Milestone M-10-00. Analysis of core samples obtained from tank 241-C-112 strongly indicates that the fuel concentration in the tank waste will not support a propagating exothermic reaction. Analysis of the process history of the tank as well as studies of simulants provided valuable information about the physical and chemical condition of the waste. This information, in combination with the analysis of the tank waste, sup ports the conclusion that an exothermic reaction in tank 241-C-112 is not plausible. Therefore, the contents of tank 241-C-112 present no imminent threat to the workers at the Hanford Site, the public, or the environment from its forrocyanide inventory. Because an exothermic reaction is not credible, the consequences of this accident scenario, as promulgated by the General Accounting Office, are not applicable.

  20. Tank characterization data report: Tank 241-C-112

    International Nuclear Information System (INIS)

    Tank 241-C-112 is a Hanford Site Ferrocyanide Watch List tank that was most recently sampled in March 1992. Analyses of materials obtained from tank 241-C-112 were conducted to support the resolution of the Ferrocyanide Unreviewed Safety Question (USQ) and to support Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) Milestone M-10-00. Analysis of core samples obtained from tank 241-C-112 strongly indicates that the fuel concentration in the tank waste will not support a propagating exothermic reaction. Analysis of the process history of the tank as well as studies of simulants provided valuable information about the physical and chemical condition of the waste. This information, in combination with the analysis of the tank waste, sup ports the conclusion that an exothermic reaction in tank 241-C-112 is not plausible. Therefore, the contents of tank 241-C-112 present no imminent threat to the workers at the Hanford Site, the public, or the environment from its forrocyanide inventory. Because an exothermic reaction is not credible, the consequences of this accident scenario, as promulgated by the General Accounting Office, are not applicable

  1. Monitoring Milk Somatic Cell Counts

    Directory of Open Access Journals (Sweden)

    Gheorghe Şteţca

    2014-11-01

    Full Text Available The presence of somatic cells in milk is a widely disputed issue in milk production sector. The somatic cell counts in raw milk are a marker for the specific cow diseases such as mastitis or swollen udder. The high level of somatic cells causes physical and chemical changes to milk composition and nutritional value, and as well to milk products. Also, the mastitic milk is not proper for human consumption due to its contribution to spreading of certain diseases and food poisoning. According to these effects, EU Regulations established the maximum threshold of admitted somatic cells in raw milk to 400000 cells / mL starting with 2014. The purpose of this study was carried out in order to examine the raw milk samples provided from small farms, industrial type farms and milk processing units. There are several ways to count somatic cells in milk but the reference accepted method is the microscopic method described by the SR EN ISO 13366-1/2008. Generally samples registered values in accordance with the admissible limit. By periodical monitoring of the somatic cell count, certain technological process issues are being avoided and consumer’s health ensured.

  2. 7 CFR 58.136 - Rejected milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Rejected milk. 58.136 Section 58.136 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Milk § 58.136 Rejected milk. A plant shall reject specific milk from a producer if the milk fails...

  3. 7 CFR 1150.111 - Milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Milk. 1150.111 Section 1150.111 Agriculture Regulations... Orders; Milk), DEPARTMENT OF AGRICULTURE DAIRY PROMOTION PROGRAM Dairy Promotion and Research Order Definitions § 1150.111 Milk. Milk means any class of cow's milk produced in the United States....

  4. Tank Characterization Report for Double Shell Tank (DST) 241-AN-107

    Energy Technology Data Exchange (ETDEWEB)

    ADAMS, M.R.

    2000-03-23

    This report interprets information about the tank answering a series of six questions covering areas such as information drivers, tank history, tank comparisons, disposal implications, data quality and quantity, and unique aspects of the tank.

  5. 49 CFR 238.423 - Fuel tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Fuel tanks. 238.423 Section 238.423 Transportation....423 Fuel tanks. (a) External fuel tanks. Each type of external fuel tank must be approved by FRA's Associate Administrator for Safety upon a showing that the fuel tank provides a level of safety at...

  6. 27 CFR 25.35 - Tanks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Tanks. 25.35 Section 25.35... TREASURY LIQUORS BEER Construction and Equipment Equipment § 25.35 Tanks. Each stationary tank, vat, cask... contents of tanks or containers in lieu of providing each tank or container with a measuring device....

  7. 14 CFR 23.1013 - Oil tanks.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Oil tanks. 23.1013 Section 23.1013... tanks. (a) Installation. Each oil tank must be installed to— (1) Meet the requirements of § 23.967 (a...) Expansion space. Oil tank expansion space must be provided so that— (1) Each oil tank used with...

  8. 49 CFR 230.116 - Oil tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Oil tanks. 230.116 Section 230.116 Transportation... Locomotive Tanks § 230.116 Oil tanks. The oil tanks on oil burning steam locomotives shall be maintained free... adjacent to the fuel supply tank or in another safe location; (b) Closes automatically when tripped...

  9. Milk contamination in different points of the dairy process. ii mesophilic, psychrotrophic and proteolytic microorganisms/ Contaminação do leite em diferentes pontos da produção leiteira: ii microrganismos mesófilos, psicrotróficos e proteolíticos

    Directory of Open Access Journals (Sweden)

    Viviane Vieira Gusmão

    2004-05-01

    Full Text Available Psychrotrophics microorganisms are able to multiply under refrigeration temperatures. They can produce heat-resistant extracellular enzymes that cause organoleptic and structural changes in milk and dairy products. With the objective of determining the frequency of microorganisms in the dairy process, four farms were evaluated in Londrina city – Brazil and different points were assessed. Mesophilic (48hs at 35oC, psychrotrophic (25hs at 21oC and proteolytic (72hs at 22oC microorganisms were researched, and the proteolytic predominant microflora were determined by using the Gram stain technique. The main psychrotrophic contamination points were the residual water on milk cans, on bulk tank and bad cleaned teats, and milk cans and teats were the most important sources of proteolytics. Gram negative bacilli represented 69% of the microorganisms in the refrigerated milk that means 45% of the total proteolytics microorganisms in the dairy process. Gram negative bacilli is most frequent on bad cleaned teats, teatcups, residual water in milk cans and bulk tank. The psychrotroph count in the refrigerated milk was more than the mesophilic count, showing that that psychrotrophics are the best indicatator for refrigerated milk contamination. The psycrotrophics count at all points assessed were greater than the ideal limit (10% of the mesophilic count. Thus as refrigeration at 4oC is not sufficient to control the multiplication of the psycrotrophics group, good practices should be associated to avoid or control milk contamination by psycrotrophics.Os psicrotróficos são microrganismos que têm capacidade de multiplicação em temperaturas de refrigeração. Produzem enzimas termorresistentes como proteases e lipases que promovem alterações organolépticas no leite e comprometem a produção de derivados. Com o objetivo de determinar a freqüência desses microrganismos a produção do leite, foram estudadas 04 propriedades da região de Londrina, PR

  10. Concentrate composition for Automatic Milking Systems - Effect on milking frequency

    DEFF Research Database (Denmark)

    Madsen, J; Weisbjerg, Martin Riis; Hvelplund, Torben

    2010-01-01

    The purpose of this study was to investigate the potential of affecting milking frequency in an Automatic Milking System (AMS) by changing ingredient composition of the concentrate fed in the AMS. In six experiments, six experimental concentrates were tested against a Standard concentrate all...... the Standard concentrate. A marked effect was found on the number of visits of the cows in the AMS and the subsequent milk production in relation to composition of the concentrate. The composition of the concentrates also influenced the composition of the milk and the MR intake. Based on the overall responses...

  11. Cow's Milk Protein Allergy.

    Science.gov (United States)

    Mousan, Grace; Kamat, Deepak

    2016-10-01

    Cow's milk protein allergy (CMPA) is a common condition encountered in children with incidence estimated as 2% to 7.5% in the first year of life. Formula and breast-fed babies can present with symptoms of CMPA. It is important to accurately diagnose CMPA to avoid the consequences of either under- or overdiagnosis. CMPA is classically categorized into immunoglobulin E (IgE)- or non-IgE-mediated reaction that vary in clinical manifestations, diagnostic evaluation, and prognosis. The most commonly involved systems in patients with CMPA are gastrointestinal, skin, and respiratory. Evaluation of CMPA starts with good data gathering followed by testing if indicated. Treatment is simply by avoidance of cow's milk protein (CMP) in the child's or mother's diet, if exclusively breast-feeding. This article reviews the definition, epidemiology, risk factors, pathogenesis, clinical presentation, evaluation, management, and prognosis of CMPA and provides an overview of different options for formulas and their indication in the treatment of CMPA.

  12. Milk and oral health.

    Science.gov (United States)

    Johansson, Ingegerd; Lif Holgerson, Pernilla

    2011-01-01

    Oral health includes freedom from disease in the gums, the mucosa and the teeth. There has been a striking reduction in dental caries and periodontitis in industrialized countries, although the proportion with severe disease has remained at 10-15%, and the prevalence increases in less developed countries. If left untreated, these diseases may lead to pain, and impaired quality of life and nutritional status. Prevention and treatment need, besides traditional implementation of proper oral hygiene, sugar restriction and use of fluoride, newer cost-effective strategies. Non-sweetened dairy products, which are proven non-cariogenic, or specific bioactive components from alike sources might prove to be part of such strategies. Thus, milk proteins, such as bovine and human caseins and lactoferrin, inhibit initial attachment of cariogenic mutans streptococci to hydroxyapatite coated with saliva or purified saliva host ligands. In contrast, both bovine and human milk coated on hydroxyapatite promotes attachment of commensal Actinomyces naeslundii and other streptococci in vitro, and phosphorylated milk-derived peptides promote maintenance of tooth minerals, as shown for the β-casein-derived caseino-phosphate peptide. Observational studies are promising, but randomized clinical trials are needed to reveal if dairy products could be a complementary treatment for oral health. PMID:21335990

  13. Cows’ diet and milk composition

    OpenAIRE

    Harstad, Odd Magne; Steinshamn, Håvard

    2010-01-01

    The effect of cows' diets on milk composition is discussed, focusing on those components of particular interest for human health. First dietary sources of fatty acids and their digestion and metabolism are reviewed. Then feeding factors affecting milk fat content and fatty acid composition are discussed, with emphasis on those factors related to forage and fat supplements. The effects of diet on protein content and composition and milk content of minerals and vitamins are reviewed. Feeding st...

  14. Reliable tank gauging

    Energy Technology Data Exchange (ETDEWEB)

    Andersson, Agne [SAAB Tank Control, Houston, TX (United States)

    1998-07-01

    There are three non-contact methods for tank gauging: Ultrasonic (Sound method), Infrared (Light method) and Radar (Microwave). All these methods send out signals in a beam and are therefore good for level measurement on liquid. The method that best fits the petrochemical industry is the RADAR. It is going to be explained different methods of tank gauging by radar: F.M.C.W. (Frequency Modulated Continuous Wave), F.H.A.S.T (Fast Hardware Adaptive Signal Technology), etc. It is going to be shown which method is good for which system, what is the secret behind the difference between a good and a bad radar level gauge. There is no obvious connection between emitted power and sensitivity in radar gauging. In fact, the grater the power emitted, the larger is the inherent disturbance from the microwave transducer. The combination of common signal filtering and strong emitted power can therefore produce a disaster from in terms of sensitivity. To measure under all conditions and in all applications you must have different antenna types and antenna diagrams. SAAB has four different antenna types that in combination with different antenna diagrams can assure you of maximum performance. The number of suppliers of radar based level measuring is increasing and to choose a supplier becomes more difficult for the costumer. Therefore, some key points are going to be discussed for having this task simplified, such as: when is a radar needed? A radar is a radar? How microwave radar gauge works? Sensitivity-finding the neal? How to recognise a high sensitivity gauge? For the newcomers on the market, the limited experience makes it easy to believe that the radar gauges are the solution for all applications. However, not all tanks are suitable for radar based level measuring and some considerations in that matter are going to be also discussed. (author)

  15. TESTING OF ENHANCED CHEMICAL CLEANING OF SRS ACTUAL WASTE TANK 5F AND TANK 12H SLUDGES

    Energy Technology Data Exchange (ETDEWEB)

    Martino, C.; King, W.

    2011-08-22

    Forty three of the High Level Waste (HLW) tanks at the Savannah River Site (SRS) have internal structures that hinder removal of the last approximately five thousand gallons of waste sludge solely by mechanical means. Chemical cleaning can be utilized to dissolve the sludge heel with oxalic acid (OA) and pump the material to a separate waste tank in preparation for final disposition. This dissolved sludge material is pH adjusted downstream of the dissolution process, precipitating the sludge components along with sodium oxalate solids. The large quantities of sodium oxalate and other metal oxalates formed impact downstream processes by requiring additional washing during sludge batch preparation and increase the amount of material that must be processed in the tank farm evaporator systems and the Saltstone Processing Facility. Enhanced Chemical Cleaning (ECC) was identified as a potential method for greatly reducing the impact of oxalate additions to the SRS Tank Farms without adding additional components to the waste that would extend processing or increase waste form volumes. In support of Savannah River Site (SRS) tank closure efforts, the Savannah River National Laboratory (SRNL) conducted Real Waste Testing (RWT) to evaluate an alternative to the baseline 8 wt. % OA chemical cleaning technology for tank sludge heel removal. The baseline OA technology results in the addition of significant volumes of oxalate salts to the SRS tank farm and there is insufficient space to accommodate the neutralized streams resulting from the treatment of the multiple remaining waste tanks requiring closure. ECC is a promising alternative to bulk OA cleaning, which utilizes a more dilute OA (nominally 2 wt. % at a pH of around 2) and an oxalate destruction technology. The technology is being adapted by AREVA from their decontamination technology for Nuclear Power Plant secondary side scale removal. This report contains results from the SRNL small scale testing of the ECC process

  16. Testing Of Enhanced Chemical Cleaning Of SRS Actual Waste Tank 5F And Tank 12H Sludges

    International Nuclear Information System (INIS)

    Forty three of the High Level Waste (HLW) tanks at the Savannah River Site (SRS) have internal structures that hinder removal of the last approximately five thousand gallons of waste sludge solely by mechanical means. Chemical cleaning can be utilized to dissolve the sludge heel with oxalic acid (OA) and pump the material to a separate waste tank in preparation for final disposition. This dissolved sludge material is pH adjusted downstream of the dissolution process, precipitating the sludge components along with sodium oxalate solids. The large quantities of sodium oxalate and other metal oxalates formed impact downstream processes by requiring additional washing during sludge batch preparation and increase the amount of material that must be processed in the tank farm evaporator systems and the Saltstone Processing Facility. Enhanced Chemical Cleaning (ECC) was identified as a potential method for greatly reducing the impact of oxalate additions to the SRS Tank Farms without adding additional components to the waste that would extend processing or increase waste form volumes. In support of Savannah River Site (SRS) tank closure efforts, the Savannah River National Laboratory (SRNL) conducted Real Waste Testing (RWT) to evaluate an alternative to the baseline 8 wt. % OA chemical cleaning technology for tank sludge heel removal. The baseline OA technology results in the addition of significant volumes of oxalate salts to the SRS tank farm and there is insufficient space to accommodate the neutralized streams resulting from the treatment of the multiple remaining waste tanks requiring closure. ECC is a promising alternative to bulk OA cleaning, which utilizes a more dilute OA (nominally 2 wt. % at a pH of around 2) and an oxalate destruction technology. The technology is being adapted by AREVA from their decontamination technology for Nuclear Power Plant secondary side scale removal. This report contains results from the SRNL small scale testing of the ECC process

  17. Regulated underground storage tanks

    International Nuclear Information System (INIS)

    This guidance package is designed to assist DOE Field operations by providing thorough guidance on the underground storage tank (UST) regulations. [40 CFR 280]. The guidance uses tables, flowcharts, and checklists to provide a ''roadmap'' for DOE staff who are responsible for supervising UST operations. This package is tailored to address the issues facing DOE facilities. DOE staff should use this guidance as: An overview of the regulations for UST installation and operation; a comprehensive step-by-step guidance for the process of owning and operating an UST, from installation to closure; and a quick, ready-reference guide for any specific topic concerning UST ownership or operation

  18. Tank closure reducing grout

    Energy Technology Data Exchange (ETDEWEB)

    Caldwell, T.B.

    1997-04-18

    A reducing grout has been developed for closing high level waste tanks at the Savannah River Site in Aiken, South Carolina. The grout has a low redox potential, which minimizes the mobility of Sr{sup 90}, the radionuclide with the highest dose potential after closure. The grout also has a high pH which reduces the solubility of the plutonium isotopes. The grout has a high compressive strength and low permeability, which enhances its ability to limit the migration of contaminants after closure. The grout was designed and tested by Construction Technology Laboratories, Inc. Placement methods were developed by the Savannah River Site personnel.

  19. Human Milk Fortification in India.

    Science.gov (United States)

    Kler, Neelam; Thakur, Anup; Modi, Manoj; Kaur, Avneet; Garg, Pankaj; Soni, Arun; Saluja, Satish

    2015-01-01

    Human milk fortification in preterm babies has become a standard of care in developed countries. Use of human milk fortifier (HMF) in very-low-birthweight infants is not a routine practice in India. There are concerns about high osmolality, feed intolerance, necrotizing enterocolitis, risk of contamination and added cost associated with use of HMF. There are limited data from India which address the issue of safety and short-term benefits of human milk fortification. This chapter highlights the issues related to human milk fortification in our country. PMID:26111571

  20. Automatic milking systems, farm size, and milk production.

    Science.gov (United States)

    Rotz, C A; Coiner, C U; Soder, K J

    2003-12-01

    Automatic milking systems (AMS) offer relief from the demanding routine of milking. Although many AMS are in use in Europe and a few are used in the United States, the potential benefit for American farms is uncertain. A farm-simulation model was used to determine the long-term, whole-farm effect of implementing AMS on farm sizes of 30 to 270 cows. Highest farm net return to management and unpaid factors was when AMS were used at maximal milking capacity. Adding stalls to increase milking frequency and possibly increase production generally did not improve net return. Compared with new traditional milking systems, the greatest potential economic benefit was a single-stall AMS on a farm size of 60 cows at a moderate milk production level (8600 kg/cow). On other farm sizes using single-stall type robotic units, losses in annual net return of 0 dollars to 300 dollars/cow were projected, with the greatest losses on larger farms and at high milk production (10,900 kg/cow). Systems with one robot serving multiple stalls provided a greater net return than single-stall systems, and this net return was competitive with traditional parlors for 50- to 130-cow farm sizes. The potential benefit of AMS was improved by 100 dollars/cow per year if the AMS increased production an additional 5%. A 20% reduction in initial equipment cost or doubling milking labor cost also improved annual net return of an AMS by up to 100 dollars/cow. Annual net return was reduced by 110 dollars/cow, though, if the economic life of the AMS was reduced by 3 yr for a more rapid depreciation than that normally used with traditional milking systems. Thus, under current assumptions, the economic return for an AMS was similar to that of new parlor systems on smaller farms when the milking capacity of the AMS was well matched to herd size and milk production level.

  1. Phase Chemistry of Tank Sludge Residual Components

    Energy Technology Data Exchange (ETDEWEB)

    KRUMHANSL,JAMES L.; LIU,JUN; NAGY,KATHRYN L.; BRADY,PATRICK V.

    1999-11-29

    We are attempting to understand the solid phase chemistry of the high level nuclear waste (HLW) stored in tanks at Hanford. Because this waste is compositionally complex, our approach is to study experimentally the aging dynamics of simplified systems whose bulk chemistry approximates that of the tank sludges. After a basic understanding of these dynamics has been attained we plan to increase the compositional complexities one component at a time, in order to assess the influence of each component. Results will allow for reliable prediction of sludge phase chemistry over a range of sludge compositions. Iron and aluminum comprise the bulk of most HLW sludges, so we chose to begin by studying the behavior of iron-aluminum systems. Fe/Al ratios were chosen to approximate those relevant to the solutions that produced the sludge. Aluminum and iron concentrations in the various process fluids are summarized and compared to our experimental starting solutions in Table 1 (process solution data from Krumhansl, personal communication, 1998). Our low aluminum experiments serve as direct analogues to both Bismuth Phosphate and low-Fe PUREX waste. Cornell and Giovanoli (1985) found that, in a pure iron system at 70 C, a 10-fold or even 50-fold increase in suspension concentration had only very slight effects on the final aged products. Since our experiments have similar Al/Fe ratios to some high Fe-PUREX process solutions our results are probably relevant to those wastes as well. However, our results may not apply to the high-Fe and high-Al PUREX wastes, as discussed below. The high Al experiments were designed specifically to simulate REDOX waste.

  2. Hanford waste tank cone penetrometer

    International Nuclear Information System (INIS)

    A new tool is being developed to characterize tank waste at the Hanford Reservation. This tool, known as the cone penetrometer, is capable of obtaining chemical and physical properties in situ. For the past 50 years, this tool has been used extensively in soil applications and now has been modified for usage in Hanford Underground Storage tanks. These modifications include development of new ''waste'' data models as well as hardware design changes to accommodate the hazardous and radioactive environment of the tanks. The modified cone penetrometer is scheduled to be deployed at Hanford by Fall 1996. At Hanford, the cone penetrometer will be used as an instrumented pipe which measures chemical and physical properties as it pushes through tank waste. Physical data, such as tank waste stratification and mechanical properties, is obtained through three sensors measuring tip pressure, sleeve friction and pore pressure. Chemical data, such as chemical speciation, is measured using a Raman spectroscopy sensor. The sensor package contains other instrumentation as well, including a tip and side temperature sensor, tank bottom detection and an inclinometer. Once the cone penetrometer has reached the bottom of the tank, a moisture probe will be inserted into the pipe. This probe is used to measure waste moisture content, water level, waste surface moisture and tank temperature. This paper discusses the development of this new measurement system. Data from the cone penetrometer will aid in the selection of sampling tools, waste tank retrieval process, and addressing various tank safety issues. This paper will explore various waste models as well as the challenges associated with tank environment

  3. Static Stress Analysis of Security Injection Tank

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The static structural analysis of the security injection tank is made to make sure whether the tank can withstand concerned loads or not on all conditions conforming to concerned code prescripts and design requirements. The tanks

  4. Oil Storage Facilities - Storage Tank Locations

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — A Storage Tank Location is a DEP primary facility type, and its sole sub-facility is the storage tank itself. Storage tanks are aboveground or underground, and are...

  5. Lactose intolerance and cow's milk protein allergy

    OpenAIRE

    Adriano Henrique do Nascimento RANGEL; Danielle Cavalcanti SALES; Urbano, Stela Antas; José Geraldo Bezerra GALVÃO JÚNIOR; Júlio César de ANDRADE NETO; Cláudia de Souza MACÊDO

    2016-01-01

    Abstract Adverse reactions to food intake have very diverse etiology and symptomatology. Regarding milk, its food allergy is presented as lactose intolerance, the sugar in milk, or allergy to milk protein. Despite having different symptomatology, confusions among allergic conditions to dairy and its mediators are common. Milk protein allergy originates from protein components present in milk, causing reactions to either the protein fractions in emulsion (caseins) or in whey (milk albumin). Th...

  6. Milk metabolites and neurodegeneration: Is there crosstalk?

    OpenAIRE

    Thakur, Keshav; Anand, Akshay

    2015-01-01

    Milk has been considered as a natural source of nutrition for decades. Milk is known to be nutrient-rich which aids the growth and development of the human body. Milk contains both macro- and micronutrients. Breast milk is widely regarded as the optimal source of neonatal nutrition due to its composition of carbohydrates, proteins, minerals and antibodies. However, despite the wide use of milk products, investigations into the role of milk in degenerative diseases have been limited. This revi...

  7. CHARACTERIZATION AND ACTUAL WASTE TEST WITH TANK 5F SAMPLES

    International Nuclear Information System (INIS)

    The initial phase of bulk waste removal operations was recently completed in Tank 5F. Video inspection of the tank indicates several mounds of sludge still remain in the tank. Additionally, a mound of white solids was observed under Riser 5. In support of chemical cleaning and heel removal programs, samples of the sludge and the mound of white solids were obtained from the tank for characterization and testing. A core sample of the sludge and Super Snapper sample of the white solids were characterized. A supernate dip sample from Tank 7F was also characterized. A portion of the sludge was used in two tank cleaning tests using oxalic acid at 50 C and 75 C. The filtered oxalic acid from the tank cleaning tests was subsequently neutralized by addition to a simulated Tank 7F supernate. Solids and liquid samples from the tank cleaning test and neutralization test were characterized. A separate report documents the results of the gas generation from the tank cleaning test using oxalic acid and Tank 5F sludge. The characterization results for the Tank 5F sludge sample (FTF-05-06-55) appear quite good with respect to the tight precision of the sample replicates, good results for the glass standards, and minimal contamination found in the blanks and glass standards. The aqua regia and sodium peroxide fusion data also show good agreement between the two dissolution methods. Iron dominates the sludge composition with other major contributors being uranium, manganese, nickel, sodium, aluminum, and silicon. The low sodium value for the sludge reflects the absence of supernate present in the sample due to the core sampler employed for obtaining the sample. The XRD and CSEM results for the Super Snapper salt sample (i.e., white solids) from Tank 5F (FTF-05-07-1) indicate the material contains hydrated sodium carbonate and bicarbonate salts along with some aluminum hydroxide. These compounds likely precipitated from the supernate in the tank. A solubility test showed the material

  8. Brucellosis in Milk and Milk Products and Its Importance

    OpenAIRE

    Keskin, Dilek; TOROĞLU, Sevil

    2007-01-01

    Brucellosis, caused by Brucella spp, is a zoonotic disease which is patogenic to human. Brucella spp especially can be host cattle, sheep, goats, water bufalo pigs, dogs, camel, deer, certain poultry and human even tick and some artropod. Brucella spp are very important for public health because, along causing important economical losses, they infect man via milk and milk products of infected animals.

  9. Enhanced Waste Tank Level Model

    Energy Technology Data Exchange (ETDEWEB)

    Duignan, M.R.

    1999-06-24

    'With the increased sensitivity of waste-level measurements in the H-Area Tanks and with periods of isolation, when no mass transfer occurred for certain tanks, waste-level changes have been recorded with are unexplained.'

  10. Modelling of baffled stirred tanks

    Energy Technology Data Exchange (ETDEWEB)

    Ahlstedt, H.; Lahtinen, M. [Tampere Univ. of Technology (Finland). Energy and Process Engineering

    1996-12-31

    The three-dimensional flow field of a baffled stirred tank has been calculated using four different turbulence models. The tank is driven by a Rushton-type impeller. The boundary condition for the impeller region has been given as a source term or by calculating the impeller using the sliding mesh technique. Calculated values have been compared with measured data. (author)

  11. Solitons in a wave tank

    DEFF Research Database (Denmark)

    Olsen, M.; Smith, H.; Scott, Alwyn C.

    1984-01-01

    A wave tank experiment (first described by the nineteenth-century engineer and naval architect John Scott Russell) relates a linear eigenvalue problem from elementary quantum mechanics to a striking feature of modern nonlinear wave theory: multiple generation of solitons. The tank experiment...

  12. Cleaning Validation of Fermentation Tanks

    DEFF Research Database (Denmark)

    Salo, Satu; Friis, Alan; Wirtanen, Gun

    2008-01-01

    Reliable test methods for checking cleanliness are needed to evaluate and validate the cleaning process of fermentation tanks. Pilot scale tanks were used to test the applicability of various methods for this purpose. The methods found to be suitable for validation of the clenlinees were visula...

  13. Mining the bulk positron lifetime

    Energy Technology Data Exchange (ETDEWEB)

    Aourag, H.; Guittom, A. [Centre de Recherche Nucleaire d' Alger (CRNA), Alger Gare - Algiers (Algeria)

    2009-02-15

    We introduce a new approach to investigate the bulk positron lifetimes of new systems based on data-mining techniques. Through data mining of bulk positron lifetimes, we demonstrate the ability to predict the positron lifetimes of new semiconductors on the basis of available semiconductor data already studied. Informatics techniques have been applied to bulk positron lifetimes for different tetrahedrally bounded semiconductors in order to discover computational design rules. (copyright 2009 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  14. Mining the bulk positron lifetime

    International Nuclear Information System (INIS)

    We introduce a new approach to investigate the bulk positron lifetimes of new systems based on data-mining techniques. Through data mining of bulk positron lifetimes, we demonstrate the ability to predict the positron lifetimes of new semiconductors on the basis of available semiconductor data already studied. Informatics techniques have been applied to bulk positron lifetimes for different tetrahedrally bounded semiconductors in order to discover computational design rules. (copyright 2009 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  15. Advances in bulk port development

    Energy Technology Data Exchange (ETDEWEB)

    Soros, P. (Soros Associates Consulting Engineers, New York, NY (USA))

    1991-03-01

    The article features several recently developed bulk ports which illustrate aspects of new technology or concepts in maritime transport. Low handling capacity bulk terminals at Ponta da Madeira, Brazil and Kooragang Island, Australia and the low-cost bulk port at Port of Corpus Christi, Texas are described. Operations at the ports of Pecket and Tocopilla in Chile, which had special technical problems, are mentioned. Coal terminals at Port Kembla, Australia and St. Johns River in Florid Jacksonville, Florida are featured as examples of terminals which had to be designed to meet high environmental standards. 13 refs., 2 figs., 14 photos.

  16. Thermal stratification in a hot water tank established by heat loss from the tank

    DEFF Research Database (Denmark)

    Fan, Jianhua; Furbo, Simon

    2012-01-01

    and the corresponding upward flow in the central parts of the tank. Tank design parameters such as tank volume, height to diameter ratio and insulation and different initial conditions of the tank are investigated.It is elucidated how thermal stratification in the tank is influenced by the natural convection and how...... to diameter ratio, tank insulation and initial conditions of the tank. The equation is validated for a 150–500l tank insulated with 0–7cm mineral wool and a tank height to diameter ratio of 1–5. The equation will be implemented in an existing tank optimization and design program for calculation of thermal...

  17. Competitive Think Tanks in Europe

    DEFF Research Database (Denmark)

    Kelstrup, Jesper Dahl

    in opportunity structures that are mediated by historically constituted institutions in knowledge regimes. The paper distinguishes between four different strategies, the authoritative, the collaborative, the agenda-setting and the competitive strategy that are distinguished by the relations think tanks have...... to established institutions and power in public policy. On the basis of the hypothesis that more competitive think tanks have emerged due to lower opportunity costs, the paper investigates how ‘competitive’ think tank strategies have been used in Germany, Denmark, the EU-institutions in Brussels...... and in the United Kingdom from 2000 to 2012. The findings contradict the hypothesis that the competitive think tank strategy is the dominant or even a common strategy across the cases under investigation. The competitive strategy is particularly rare among EU and German think tanks. As such the paper challenges...

  18. Hanford site waste tank characterization

    International Nuclear Information System (INIS)

    This paper describes the on-going work in the characterization of the Hanford-Site high-level waste tanks. The waste in these tanks was produced as part of the nuclear weapons materials processing mission that occupied the Hanford Site for the first 40 years of its existence. Detailed and defensible characterization of the tank wastes is required to guide retrieval, pretreatment, and disposal technology development, to address waste stability and reactivity concerns, and to satisfy the compliance criteria for the various regulatory agencies overseeing activities at the Hanford Site. The resulting Tank Characterization Reports fulfill these needs, as well as satisfy the tank waste characterization milestones in the Hanford Federal Facility Agreement and Consent Order

  19. Methods for determination of milk and cheese adulteration by other milk types

    OpenAIRE

    Dubravka Samaržija; Sonja Damjanović; Jasmina Havranek

    2006-01-01

    In the world milk production, the contribution of goat, ovine, buffalo and other types of milk is small, compared to the cows' milk. Because of great availability, cows' milk is often used for adulteration of other milk types and dairy products. Due to adulteration, food characteristics are changed. Several analytical techniques were reported in the literature for the detection of milk and dairy products adulteration. Most of them are based on detection of milk protein fractions. Methods base...

  20. Characterization of contaminants from a sanitized milk processing plant.

    Directory of Open Access Journals (Sweden)

    Sara Cleto

    Full Text Available Milk processing lines offer a wide variety of microenvironments where a diversity of microorganisms can proliferate. We sampled crevices and junctions where, due to deficient reach by typical sanitizing procedures, bacteria can survive and establish biofilms. The sampling sites were the holding cell, cold storage tank, pasteurizer and storage tank--transfer pump junction. The culturable bacteria that were isolated after the sanitation procedure were predominantly Pseudomonas spp., Serratia spp, Staphylococcus sciuri and Stenotrophomonas maltophilia. We assayed several phenotypic characteristics such as the ability to secrete enzymes and siderophores, as well as the capacity of the strains to form biofilms that might contribute to their survival in a mixed species environment. The Pseudomonas spp. isolates were found to either produce proteases or lecithinases at high levels. Interestingly, protease production showed an inverse correlation with siderophore production. Furthermore, all of the Serratia spp. isolates were strong biofilm formers and spoilage enzymes producers. The organisms identified were not mere contaminants, but also producers of proteins with the potential to lower the quality and shelf-life of milk. In addition, we found that a considerable number of the Serratia and Pseudomonas spp. isolated from the pasteurizer were capable of secreting compounds with antimicrobial properties.

  1. Radiopharmaceuticals in breast milk

    International Nuclear Information System (INIS)

    As assessment has been made of the radiological hazards to an infant following the administration of a radiopharmaceutical to a breast feeding mother. Feeding should be discontinued after administration of most I-131 and I-125 compounds, Ga-67 citrate or Se-78 methionine, and for iodinated compounds where it was possible to resume feeding, a thyroid-blocking agent should be administered. For Tc-99m compounds, pertechnetate had the greatest excretion in milk and interruptions of 12hr and 4hr were considered appropriate for pertechnetate and MAA respectively. Other Tc-99m compounds, Cr-51 EDTA and In-111 leucocytes did not justify an interruption just on the grounds of their associated excretion in milk. The ingestion hazard could be minimized by reducing the administered activity, and in some cases, by the substitution of a radiopharmaceutical with lower breast milk excretion. For Tc-99m lung and brain scans, the absorbed dose due to radiation emitted by the mother (i.e. when cuddling) was less than the ingested dose, but for a Tc-99m bone scan the emitted dose was greater. In all three cases, the emitted dose did not exceed 0 x 5 mGy for the infant in close contact to the mother for one-third of the time. For In-111 leucocytes, the emitted dose was about 2mGy, and it was concluded that close contact should be restricted to feeding times during the first 3 days after injection. 36 references, 2 figures, 5 tables

  2. Estimating Residual Solids Volume In Underground Storage Tanks

    International Nuclear Information System (INIS)

    The Savannah River Site liquid waste system consists of multiple facilities to safely receive and store legacy radioactive waste, treat, and permanently dispose waste. The large underground storage tanks and associated equipment, known as the 'tank farms', include a complex interconnected transfer system which includes underground transfer pipelines and ancillary equipment to direct the flow of waste. The waste in the tanks is present in three forms: supernatant, sludge, and salt. The supernatant is a multi-component aqueous mixture, while sludge is a gel-like substance which consists of insoluble solids and entrapped supernatant. The waste from these tanks is retrieved and treated as sludge or salt. The high level (radioactive) fraction of the waste is vitrified into a glass waste form, while the low-level waste is immobilized in a cementitious grout waste form called saltstone. Once the waste is retrieved and processed, the tanks are closed via removing the bulk of the waste, chemical cleaning, heel removal, stabilizing remaining residuals with tailored grout formulations and severing/sealing external penetrations. The comprehensive liquid waste disposition system, currently managed by Savannah River Remediation, consists of 1) safe storage and retrieval of the waste as it is prepared for permanent disposition; (2) definition of the waste processing techniques utilized to separate the high-level waste fraction/low-level waste fraction; (3) disposition of LLW in saltstone; (4) disposition of the HLW in glass; and (5) closure state of the facilities, including tanks. This paper focuses on determining the effectiveness of waste removal campaigns through monitoring the volume of residual solids in the waste tanks. Volume estimates of the residual solids are performed by creating a map of the residual solids on the waste tank bottom using video and still digital images. The map is then used to calculate the volume of solids remaining in the waste tank. The ability to

  3. Estimating Residual Solids Volume In Underground Storage Tanks

    Energy Technology Data Exchange (ETDEWEB)

    Clark, Jason L.; Worthy, S. Jason; Martin, Bruce A.; Tihey, John R.

    2014-01-08

    The Savannah River Site liquid waste system consists of multiple facilities to safely receive and store legacy radioactive waste, treat, and permanently dispose waste. The large underground storage tanks and associated equipment, known as the 'tank farms', include a complex interconnected transfer system which includes underground transfer pipelines and ancillary equipment to direct the flow of waste. The waste in the tanks is present in three forms: supernatant, sludge, and salt. The supernatant is a multi-component aqueous mixture, while sludge is a gel-like substance which consists of insoluble solids and entrapped supernatant. The waste from these tanks is retrieved and treated as sludge or salt. The high level (radioactive) fraction of the waste is vitrified into a glass waste form, while the low-level waste is immobilized in a cementitious grout waste form called saltstone. Once the waste is retrieved and processed, the tanks are closed via removing the bulk of the waste, chemical cleaning, heel removal, stabilizing remaining residuals with tailored grout formulations and severing/sealing external penetrations. The comprehensive liquid waste disposition system, currently managed by Savannah River Remediation, consists of 1) safe storage and retrieval of the waste as it is prepared for permanent disposition; (2) definition of the waste processing techniques utilized to separate the high-level waste fraction/low-level waste fraction; (3) disposition of LLW in saltstone; (4) disposition of the HLW in glass; and (5) closure state of the facilities, including tanks. This paper focuses on determining the effectiveness of waste removal campaigns through monitoring the volume of residual solids in the waste tanks. Volume estimates of the residual solids are performed by creating a map of the residual solids on the waste tank bottom using video and still digital images. The map is then used to calculate the volume of solids remaining in the waste tank. The

  4. Supporting document for the Southeast Quadrant historical tank content estimate report for SY-tank farm

    International Nuclear Information System (INIS)

    Historical Tank Content Estimate of the Southeast Quadrant provides historical evaluations on a tank by tank basis of the radioactive mixed wastes stored in the underground double-shell tanks of the Hanford 200 East and West Areas. This report summarizes historical information such as waste history, temperature profiles, psychrometric data, tank integrity, inventory estimates and tank level history on a tank by tank basis. Tank Farm aerial photos and in-tank photos of each tank are provided. A brief description of instrumentation methods used for waste tank surveillance are included. Components of the data management effort, such as Waste Status and Transaction Record Summary, Tank Layer Model, Supernatant Mixing Model, Defined Waste Types, and Inventory Estimates which generate these tank content estimates, are also given in this report

  5. Supporting document for the Southeast Quadrant historical tank content estimate report for SY-tank farm

    Energy Technology Data Exchange (ETDEWEB)

    Brevick, C.H.; Gaddis, L.A.; Consort, S.D. [Westinghouse Hanford Co., Richland, WA (United States)

    1995-12-31

    Historical Tank Content Estimate of the Southeast Quadrant provides historical evaluations on a tank by tank basis of the radioactive mixed wastes stored in the underground double-shell tanks of the Hanford 200 East and West Areas. This report summarizes historical information such as waste history, temperature profiles, psychrometric data, tank integrity, inventory estimates and tank level history on a tank by tank basis. Tank Farm aerial photos and in-tank photos of each tank are provided. A brief description of instrumentation methods used for waste tank surveillance are included. Components of the data management effort, such as Waste Status and Transaction Record Summary, Tank Layer Model, Supernatant Mixing Model, Defined Waste Types, and Inventory Estimates which generate these tank content estimates, are also given in this report.

  6. Bulk Nuclear Properties from Reactions

    OpenAIRE

    Danielewicz, P.

    2002-01-01

    Extraction of bulk nuclear properties by comparing reaction observables to results from semiclassical transport-model simulations is discussed. Specific properties include the nuclear viscosity, incompressibility and constraints on the nuclear pressure at supranormal densities.

  7. Environmental Chemicals in Breast Milk

    Science.gov (United States)

    Most of the information available on environmental chemicals in breast milk is focused on persistent, lipophilic chemicals; the database on levels of these chemicals has expanded substantially since the 1950s. Currently, various types of chemicals are measured in breast milk and ...

  8. Breastfeeding, breast milk and viruses

    Directory of Open Access Journals (Sweden)

    Glenn Wendy K

    2007-10-01

    Full Text Available Abstract Background There is seemingly consistent and compelling evidence that there is no association between breastfeeding and breast cancer. An assumption follows that milk borne viruses cannot be associated with human breast cancer. We challenge this evidence because past breastfeeding studies did not determine "exposure" of newborn infants to colostrum and breast milk. Methods We conducted a prospective review of 100 consecutive births of infants in the same centre to determine the proportion of newborn infants who were "exposed" to colostrum or breast milk, as distinct from being fully breast fed. We also report a review of the breastfeeding practices of mothers of over 87,000 newborn infants in the Australian State of New South Wales. This study was approved by the Human Research Ethics Committee of the University of New South Wales (Sydney, Australia. Approval 05063, 29 September 2005. Results Virtually all (97 of 100 newborn infants in this centre were "exposed" to colostrum or breast milk whether or not they were fully breast fed. Between 82.2% to 98.7% of 87,000 newborn infants were "exposed" to colostrum or breast milk. Conclusion In some Western communities there is near universal exposure of new born infants to colostrum and breast milk. Accordingly it is possible for the transmission of human milk borne viruses. This is contrary to the widespread assumption that human milk borne viruses cannot be associated with breast cancer.

  9. Automatic milking : a better understanding

    NARCIS (Netherlands)

    Meijering, A.; Hogeveen, H.; Koning, de C.J.A.M.

    2004-01-01

    In 2000 the book Robotic Milking, reflecting the proceedings of an International Symposium which was held in The Netherlands came out. At that time, commercial introduction of automatic milking systems was no longer obstructed by technological inadequacies. Particularly in a few west-European countr

  10. Tank-automotive robotics

    Science.gov (United States)

    Lane, Gerald R.

    1999-07-01

    To provide an overview of Tank-Automotive Robotics. The briefing will contain program overviews & inter-relationships and technology challenges of TARDEC managed unmanned and robotic ground vehicle programs. Specific emphasis will focus on technology developments/approaches to achieve semi- autonomous operation and inherent chassis mobility features. Programs to be discussed include: DemoIII Experimental Unmanned Vehicle (XUV), Tactical Mobile Robotics (TMR), Intelligent Mobility, Commanders Driver Testbed, Collision Avoidance, International Ground Robotics Competition (ICGRC). Specifically, the paper will discuss unique exterior/outdoor challenges facing the IGRC competing teams and the synergy created between the IGRC and ongoing DoD semi-autonomous Unmanned Ground Vehicle and DoT Intelligent Transportation System programs. Sensor and chassis approaches to meet the IGRC challenges and obstacles will be shown and discussed. Shortfalls in performance to meet the IGRC challenges will be identified.

  11. Raw milk consumption and health

    Directory of Open Access Journals (Sweden)

    Popović-Vranješ Anka

    2015-01-01

    Full Text Available Contrary to the safe practices of milk pasteurization or sterilization, which effectively reduce foodborne outbreaks incidence associated with raw milk and dairy products use, outbreaks caused by such products continue to occur. Despite this fact, a worldwide movement advocating for the rights of raw milk and cheese selling and consumption, due to their specific nutritive characteristics, has strengthened significantly in recent years. Traditional agricultural manufacturers from Serbia still sell products related to thermally unprocessed milk, such as cottage cheese and raw cream. In AP Vojvodina during the period of 1981-2010 a total of 179 foodborne outbreaks were reported, where the incriminated cause of the outbreak were milk or diary. In 126 (70.39% outbreaks, totaling 2276 sick individuals and one casualty, it was confirmed that the incriminated food was from the group of dairy products. In 48 instances (26.82%, bacteriological tests confirmed that milk and dairy products were excluded as the outbreak causes, while in another 5 (2.79% outbreaks, microbiological analysis of food failed to confirm any relation to the actual epidemiological instances. In some cases, bacteriological testing of incriminated foods was not possible. In the cases of outbreaks associated with the consumption of milk and dairy products, traditional raw milk products were cited as being used. Consumption of unpasteurized milk and cheese represents public health threat. National and international rules ensuring use of safe products for human consumption have to set rules of trade of thermally processed milk and products on the market. [Projekat Ministarstva nauke Republike Srbije, br. TR31095

  12. Comparing milk yield, chemical properties and somatic cell count from organic and conventional mountain farming systems

    Directory of Open Access Journals (Sweden)

    Marcello Bianchi

    2010-01-01

    Full Text Available A study was undertaken to investigate the effects of farming systems (organic vs. conventional, diet (hay/concentrate vs. pasture and their interaction on milk yield, gross composition and fatty acid (FA profile of dairy cows bred in mountainous areas. For this purpose four dairy farms (two organic and two conventional were chosen in the alpine territory of Aosta Valley (NW Italy; individual milk yield was recorded daily and bulk milk samples were collected monthly from February to September 2007 to cover dietary variations. Higher levels of milk production (P<0.05 and lower milk protein amounts (P<0.01 were observed in the organic farms with respect to the conventional ones, while no significant differences were noticed in milk fat and lactose contents and in somatic cell count. Concerning fatty acids, only small differences were detected between organic and conventional milk and such differences seemed to be related mainly to the stabled period. Diet affected almost all variables studied: pasture feeding provided a significant improvement in the fatty acid composition in both organic and conventional systems leading to lower hypercholesterolemic saturated fatty acids, higher mono- and polyunsaturated fatty acids and conjugated linoleic acid amounts (P<0.001.

  13. Tank farms hazards assessment

    International Nuclear Information System (INIS)

    Hanford contractors are writing new facility specific emergency procedures in response to new and revised US Department of Energy (DOE) Orders on emergency preparedness. Emergency procedures are required for each Hanford facility that has the potential to exceed the criteria for the lowest level emergency, an Alert. The set includes: (1) a facility specific procedure on Recognition and Classification of Emergencies, (2) area procedures on Initial Emergency Response and, (3) an area procedure on Protective Action Guidance. The first steps in developing these procedures are to identify the hazards at each facility, identify the conditions that could release the hazardous material, and calculate the consequences of the releases. These steps are called a Hazards Assessment. The final product is a document that is similar in some respects to a Safety Analysis Report (SAR). The document could br produced in a month for a simple facility but could take much longer for a complex facility. Hanford has both types of facilities. A strategy has been adopted to permit completion of the first version of the new emergency procedures before all the facility hazards Assessments are complete. The procedures will initially be based on input from a task group for each facility. This strategy will but improved emergency procedures in place sooner and therefore enhance Hanford emergency preparedness. The purpose of this document is to summarize the applicable information contained within the Waste Tank Facility ''Interim Safety Basis Document, WHC-SD-WM-ISB-001'' as a resource, since the SARs covering Waste Tank Operations are not current in all cases. This hazards assessment serves to collect, organize, document and present the information utilized during the determination process

  14. Effect of concentrate feeding on milking frequency and milk yield in an automatic milking system

    Directory of Open Access Journals (Sweden)

    F. Calza

    2010-01-01

    Full Text Available The application of Automatic Milking Systems (AMS represents a substantial innovation in dairy cattle. The introduction of this new technology puts some questions about cow management. A correct and regular flow of animals through the milking unit appears fundamental to improve efficiency of AMS, animal welfare and performances.

  15. Effect of concentrate feeding on milking frequency and milk yield in an automatic milking system

    OpenAIRE

    F. Calza; S. Lolli; M. Speroni; L. Migliorati

    2010-01-01

    The application of Automatic Milking Systems (AMS) represents a substantial innovation in dairy cattle. The introduction of this new technology puts some questions about cow management. A correct and regular flow of animals through the milking unit appears fundamental to improve efficiency of AMS, animal welfare and performances.

  16. Investigations on a milk cooling plant with heat recovery. Untersuchungen an einer Milchkuehlanlage mit Waermerueckgewinnung

    Energy Technology Data Exchange (ETDEWEB)

    Loechel, E.; Bache, E. (Bitzer Kuehlmaschinenbau Schkeuditz GmbH (Germany))

    1991-01-01

    In the scope of an investigation program, the refrigeration technological, chemical, biological and sanitary prerequisites of milk cooling have been investigated. The condition was the cooling of 1000 l of milk in a milk cooling tub with a content of 2000 l. The cooling was realized in mechanical milkers according to DIN 8968 and ISO 5708. The tests began with a plant without accumulting the refrigerant in the heat recovery tank or without by-pass conduit from the compressor to the collector, which could be, however, retrofitted on demand. Based on the results received, additional measures have been ignored, since the profit bears no relation to the disadvantages (price of the plant, complexity of the control, incrase of the susceptibility and the leakage of the plant). The tests are carried out with R12 as well as with R22. (BWI).

  17. Tocopherol, carotenoids and fatty acid composition in organic and conventional milk

    DEFF Research Database (Denmark)

    Slots, Tina; Sørensen, John; Nielsen, Jacob Holm

    2008-01-01

    Organic and conventional milk from silo tanks on Danish dairy plants was compared monthly during one year. The contents of α-tocopherol, carotenoids and α-linolenic acid (C18:3 ω-3) were higher in organic milk, where the content of synthetic 2R-stereo-isomers of α-tocopherol, the ratio between ω-6...... fatty acids and ω-3 fatty acids (ω-6/ω-3) and of the content of oleic acid (C18:1 (c9)) were higher in the conventional milk. The contents of α-tocopherol, the natural stereoisomer RRR-α-tocopherol, the carotenoids (β-carotene, lutein and zeaxanthine), almost all of the saturated fatty acids and of α...

  18. Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

    OpenAIRE

    Sakti, Setyawan P.; Nur Chabibah; Ayu, Senja P.; Masdiana C. Padaga; Aulanni’am Aulanni’am

    2016-01-01

    Adulteration of goat milk is usually done using cow’s milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence o...

  19. Bacteriological examination of milk and milk products sold in Harare.

    Science.gov (United States)

    Igumbor, E O; Obi, C L; Milingo, T

    2000-01-01

    A study to assess the bacteriological quality of milk and ice cream was conducted using the direct plate count method and the methylene blue dye reduction test. A total of 105 milk and 95 ice cream samples were obtained form two factories (depots) and distributing supermarkets (outlets) in Harare. Under the methylene blue test, all milk and ice cream samples passed the hour and 2 hour tests respectively. However, 99% of the milk and 69% of the ice cream samples reduced the dye after 5.5 and 4 hrs respectively. The results from the direct plate counts revealed the presence of both pathogens and non-pathogens. The median plate counts in the milk and ice cream were found 400 cfu / ml and 100 cfu / ml respectively. Organisms isolated in both samples and in all outlets were similar, these included Bacillus spp. Coagulase Staphlococcus spp., microcuccus spp., Steptococcus spp., Diphthroids, Fusiform bacterial Klebsiella spp., and Citrobacter spp. No significant differences were found in the plate counts of the samples obtained from the depots and outlets for the milk (P = 0.542, df = 1)) and ice cream samples (P = 0.377, df = 1). Results further revealed that there was no significant difference in isolates obtained form strawberry ice cream (0.0096). The study has therefore, revealed that milk and milk product sold in various outlets in Harare contained a variety of bacteria of public health importance and also that the methylene blue dye reduction test is not reliable for the detection of bacterial contaminants in dairy products. It is thus suggested that the use of methylene blue dyes be adapted in combination with other tests such as the plate count in assessing bacterial contaminants in milk products. PMID:17650038

  20. Amiata donkey milk chain: animal health evaluation and milk quality

    Directory of Open Access Journals (Sweden)

    Giuseppe Ragona

    2016-08-01

    Full Text Available This study presents an investigation of Amiata donkey health and quality of milk for human consumption. Thirty-one lactating dairy jennies were examined. The following samples were collected: faecal samples from the rectum of animals for parasitological examination; cervical swabs for the detection of bacteria causing reproductive disorders; and blood samples for serological diagnosis of main zoonotic (Brucella spp., Leptospira spp. and donkey abortion agents (Brucella spp., Leptospira spp., Salmonella abortus equi, Equine viral arterithis virus, Equine herpesvirus type 1. In addition, individual milk samples were collected and analysed for mastitis- causing pathogens and milk quality. Regarding animal health, we detected a high prevalence of strongyle parasites in donkeys. It is very important to tackle parasitic diseases correctly. Selective control programmes are preferable in order to reduce anthelmintic drug use. For dairy donkeys, withdrawal periods from anthelmintic drugs need to be carefully managed, in accordance with EU and national regulations. The isolation of Staphylococcus aureus in milk highlights the importance of preventing contamination during milking, by adopting appropriate hygiene and safety practices at a farm level. Lysozyme activity was high compared to cow’s milk, contributing to the inhibitory activity against certain bacteria. Donkey milk was characterised by high lactose content, low caseins, low fat, higher levels of unsaturated fatty acids compared to ruminant milks. Unsaturated fatty acids and omega 3 fatty acids in particular have become known for their beneficial health effect, which is favourable for human diet. These characteristics make it suitable for infants and children affected by food intolerance/ allergies to bovine milk proteins and multiple food allergies as well as for adults with dyslipidemias. It is also recommended to prevent cardiovascular diseases.

  1. Milk from different species: Relationship between protein fractions and inflammatory response in infants affected by generalized epilepsy.

    Science.gov (United States)

    Albenzio, M; Santillo, A; Ciliberti, M G; Figliola, L; Caroprese, M; Marino, R; Polito, A N

    2016-07-01

    The present study was undertaken to evaluate the effect of protein fractions from bovine, caprine, and ovine milk on production of cytokines and reactive oxygen species (ROS) and reactive nitrogen species (RNS) by cultured peripheral blood mononuclear cells (PMBC) from infants with generalized epilepsy. Bovine, caprine, and ovine bulk milks were pasteurized and analyzed for chemical composition. Then, PBMC were isolated from 10 patients with generalized epilepsy (5 males; mean age 33.6±5.4mo). Production of tumor necrosis factor-α (TNF-α), IL-10, IL-6, and IL-1β was studied in cultured PBMC (from infants with epilepsy and controls) stimulated by bovine, caprine, and ovine milk and casein and whey protein fractions, and levels of ROS and RNS were measured in the culture supernatant. The ability of PBMC to secrete cytokines in response to milk and protein fraction stimulation may predict the secretion of soluble factor TNF-α in the bloodstream of challenged patients. Bovine, caprine, and ovine bulk milks induced low-level production of IL-10 by cultured PBMC in at least 50% of cases; the same behavior was observed in both casein and whey protein fractions for all species studied. Bovine and ovine milk and their casein fractions induced production of lower levels of IL-1β in 80% of patients, whereas caprine milk and its casein fraction induced the highest levels in 80% of patients. The amount of IL-6 detected after stimulation of PBMC by milk and its fractions for all species was lower than that of other proinflammatory cytokines. In the bovine, total free radicals were higher in bulk milk and lower in the casein fraction, whereas the whey protein fraction showed an intermediate level; in caprine, ROS/RNS levels were not different among milk fractions, whereas ovine had higher levels for bulk milk and casein than the whey protein fraction. Lower levels of ROS/RNS detected in PBMC cultured with caprine milk fraction could be responsible for the lower levels of

  2. Milk from different species: Relationship between protein fractions and inflammatory response in infants affected by generalized epilepsy.

    Science.gov (United States)

    Albenzio, M; Santillo, A; Ciliberti, M G; Figliola, L; Caroprese, M; Marino, R; Polito, A N

    2016-07-01

    The present study was undertaken to evaluate the effect of protein fractions from bovine, caprine, and ovine milk on production of cytokines and reactive oxygen species (ROS) and reactive nitrogen species (RNS) by cultured peripheral blood mononuclear cells (PMBC) from infants with generalized epilepsy. Bovine, caprine, and ovine bulk milks were pasteurized and analyzed for chemical composition. Then, PBMC were isolated from 10 patients with generalized epilepsy (5 males; mean age 33.6±5.4mo). Production of tumor necrosis factor-α (TNF-α), IL-10, IL-6, and IL-1β was studied in cultured PBMC (from infants with epilepsy and controls) stimulated by bovine, caprine, and ovine milk and casein and whey protein fractions, and levels of ROS and RNS were measured in the culture supernatant. The ability of PBMC to secrete cytokines in response to milk and protein fraction stimulation may predict the secretion of soluble factor TNF-α in the bloodstream of challenged patients. Bovine, caprine, and ovine bulk milks induced low-level production of IL-10 by cultured PBMC in at least 50% of cases; the same behavior was observed in both casein and whey protein fractions for all species studied. Bovine and ovine milk and their casein fractions induced production of lower levels of IL-1β in 80% of patients, whereas caprine milk and its casein fraction induced the highest levels in 80% of patients. The amount of IL-6 detected after stimulation of PBMC by milk and its fractions for all species was lower than that of other proinflammatory cytokines. In the bovine, total free radicals were higher in bulk milk and lower in the casein fraction, whereas the whey protein fraction showed an intermediate level; in caprine, ROS/RNS levels were not different among milk fractions, whereas ovine had higher levels for bulk milk and casein than the whey protein fraction. Lower levels of ROS/RNS detected in PBMC cultured with caprine milk fraction could be responsible for the lower levels of

  3. Tank vapor mitigation requirements for Hanford Tank Farms

    International Nuclear Information System (INIS)

    Westinghouse Hanford Company has contracted Los Alamos Technical Associates to listing of vapors and aerosols that are or may be emitted from the High Level Waste (HLW) tanks at Hanford. Mitigation requirements under Federal and State law, as well as DOE Orders, are included in the listing. The lists will be used to support permitting activities relative to tank farm ventilation system up-grades. This task is designated Task 108 under MJB-SWV-312057 and is an extension of efforts begun under Task 53 of Purchase Order MPB-SVV-03291 5 for Mechanical Engineering Support. The results of that task, which covered only thirty-nine tanks, are repeated here to provide a single source document for vapor mitigation requirements for all 177 HLW tanks

  4. Tank characterization report for double shell tank 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    Winkelman, W.D., Westinghouse Hanford

    1996-08-07

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-AP-104. This report supports the requirements of Tri-Party Agreement Milestone M-44-09.

  5. Tank vapor mitigation requirements for Hanford Tank Farms

    Energy Technology Data Exchange (ETDEWEB)

    Rakestraw, L.D.

    1994-11-15

    Westinghouse Hanford Company has contracted Los Alamos Technical Associates to listing of vapors and aerosols that are or may be emitted from the High Level Waste (HLW) tanks at Hanford. Mitigation requirements under Federal and State law, as well as DOE Orders, are included in the listing. The lists will be used to support permitting activities relative to tank farm ventilation system up-grades. This task is designated Task 108 under MJB-SWV-312057 and is an extension of efforts begun under Task 53 of Purchase Order MPB-SVV-03291 5 for Mechanical Engineering Support. The results of that task, which covered only thirty-nine tanks, are repeated here to provide a single source document for vapor mitigation requirements for all 177 HLW tanks.

  6. Tank characterization report for single-shell Tank B-201

    Energy Technology Data Exchange (ETDEWEB)

    Heasler, P.G.; Remund, K.M.; Tingey, J.M.; Baird, D.B.; Ryan, F.M.

    1994-09-01

    The purpose of this report is to characterize the waste in single shell Tank B-201. Characterization includes the determination of the physical, chemical (e.g., concentrations of elements and organic species), and radiological properties of the waste. These determinations are made using analytical results from B-201 core samples as well as historical information about the tank. The main objective is to determine average waste properties: but in some cases, concentrations of analytes as a function of depth were also determined. This report also consolidates the available historical information regarding Tank B-201, arranges the analytical information from the recent core sampling in a useful format, and provides an interpretation of the data within the context of what is known about the tank.

  7. Tank characterization report for single shell tank 241-S-107

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, B.C.

    1996-09-19

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-S-107. This report supports the requirements of Tri- Party Agreement Milestone M-44-09.

  8. Seasonal Variation in Compositions and Fatty Acids Profile (with Emphasis on CLA in Iranian Buffalo’s Milk

    Directory of Open Access Journals (Sweden)

    K. Reza Yazdi

    2010-02-01

    Full Text Available In this study the seasonal variations of milk compositions and fatty acids profile with emphasis on Conjugated linoleic acid (CLA and vaccinic acid (VA concentrations of buffalo’ s Milk in North of Iran was investigating. In each season (2006we had 30 samples (10 samples for each month of milk from bulk milk and sent them to laboratory for determination of It’s Compositions .Then fatty acids profile of milk fat was determined by Gas Chromatograph. The mean of Data from 120 samples for 4 seasons of year (2006 were compared using Duncan’s Multiple Range Test with an alpha of 5% and the results show that: The range of total solids (TS in milk samples were 16.15 – 18.93 percent so that high percent of TS was in April to July. There was no significant difference in amount of lactose in each of the seasons and it, S range was 4.93 –5.12 percent. The range of milk protein was 3.60 – 3.88 percent, there was significant difference between seasons so that the high content of protein was in April to July and the low content of it was in December to March. There was significant difference in content of milk fat in samples of various season .It was 6.78 – 9.25 percent, so that the high percent of milk fat was in December to June. The content of Unsaturated fatty acids was 50.50 to 58.23 percent of total milk fat .The content of Unsaturated fatty acids (C14 – C18 were higher in Spring season .These Data show That season of Year can influence on milk compositions and fatty acids profile of milk via the type and quality of forages that buffalos use it in grazing or feeding, the amount and kind of supplementation of used diet.

  9. Ecodesign of Liquid Fuel Tanks

    Science.gov (United States)

    Gicevska, Jana; Bazbauers, Gatis; Repele, Mara

    2011-01-01

    The subject of the study is a 10 litre liquid fuel tank made of metal and used for fuel storage and transportation. The study dealt with separate life cycle stages of this product, compared environmental impacts of similar fuel tanks made of metal and plastic, as well as analysed the product's end-of-life cycle stage, studying the waste treatment and disposal scenarios. The aim of this study was to find opportunities for improvement and to develop proposals for the ecodesign of 10 litre liquid fuel tank.

  10. [Death in a relaxation tank].

    Science.gov (United States)

    Rupp, Wolf; Simon, Karl-Heinz; Bohnert, Michael

    2009-01-01

    Complete relaxation can be achieved by floating in a darkened, sound-proof relaxation tank filled with salinated water kept at body temperature. Under these conditions, meditation exercises up to self-hypnosis may lead to deep relaxation with physical and mental revitalization. A user manipulated his tank, presumably to completely cut off all optical and acoustic stimuli and accidentally also covered the ventilation hole. The man was found dead in his relaxation tank. The findings suggested lack of oxygen as the cause of death.

  11. Evaluation and implementation of good pratices in main points of microbiological contamination in milk production/ Avaliação e implantação de boas práticas nos principais pontos de contaminação microbiológica na produção leiteira

    Directory of Open Access Journals (Sweden)

    Mykell S. Pereira

    2005-06-01

    Full Text Available In order to improve the microbiological quality of Brazilian milk and dairy products some measures have been implemented in the milk-producing sector throughout the years, such as milk refrigeration and bulk collection. Refrigeration is a very efficient procedure, however allows psychrotrophics multiplication, which are largely found in milk produced in poor hygienic conditions. To assure and improve the microbiologic quality of milk turns out to be necessary the implementation of hygienic measures, known as Good Manufacturing Practices (GMP. In this study, several hygienic practices were tested in the main contamination points in milk production, previously determined: teats (different chlorine concentrations pre-dipping, cans and bulk tanks (hygienic techniques and residual water (elimination. The recommended practices were picked up considering their efficiency in microbial reduction, practiced and economic viability. When the practices were all applied, reduction in microbial counts was observed. After 48 hours the final and refrigerated milk showed a reduction of 11.95x106 CFU/mL to 12.48x103 CFU/mL of mesophilic aerobes, and a reduction of 18.10x106 CFU/mL to 5.38x103 CFU/mL of psychrotrophics. The results show that the procedures proposed by LIPOA is efficient, viable and easily adopted by milk farmers, representing an important alternative in producing high quality milk.Buscando a melhoria da qualidade microbiológica de leite e derivados produzidos no Brasil, nos últimos anos algumas medidas vêm sendo implantadas na produção leiteira como a refrigeração e coleta a granel. A refrigeração é um procedimento eficiente no controle de aeróbios mesófilos, mas permite multiplicação de psicrotróficos, encontrados em altas contagens em leite produzido com pouca higiene. Para garantia e melhoraria da qualidade microbiológica do leite é necessária a implantação de medidas higiênicas, conhecidas como Boas Práticas de Produ

  12. 7 CFR 58.233 - Skim milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Skim milk. 58.233 Section 58.233 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Materials § 58.233 Skim milk. The skim milk shall be separated from whole milk meeting the requirements...

  13. 7 CFR 1005.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1005.13 Section 1005.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE APPALACHIAN MARKETING AREA Order Regulating Handling Definitions § 1005.13 Producer milk. Except as provided for in paragraph (e) of this...

  14. 7 CFR 58.936 - Milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Milk. 58.936 Section 58.936 Agriculture Regulations of... Official Identification § 58.936 Milk. To process and package evaporated and condensed milk of ultra... Shield the raw incoming milk shall meet the requirements as outlined in §§ 58.132 through 58.136....

  15. 7 CFR 1124.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1124.13 Section 1124.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE PACIFIC NORTHWEST MARKETING AREA Order Regulating Handling Definitions § 1124.13 Producer milk. Except as provided for in paragraph (f) of...

  16. 7 CFR 58.430 - Milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Milk. 58.430 Section 58.430 Agriculture Regulations of....430 Milk. The milk shall be fresh, sweet, pleasing and desirable in flavor and shall meet the requirements as outlined under §§ 58.132 through 58.138. The milk may be adjusted by separating part of the...

  17. 7 CFR 1030.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1030.13 Section 1030.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE UPPER MIDWEST MARKETING AREA Order Regulating Handling Definitions § 1030.13 Producer milk. Except as provided for in paragraph (e) of this...

  18. 7 CFR 1007.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Producer milk. 1007.13 Section 1007.13 Agriculture... and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE SOUTHEAST MARKETING AREA Order Regulating Handling Definitions § 1007.13 Producer milk. Except as provided for in paragraph (e) of this...

  19. Milk Pricing at the Wholesale Level

    OpenAIRE

    Christensen, Rondo A.

    1987-01-01

    Although the average marketing margin for fluid milk products (retail price minus raw milk price) has increased with marketing costs in recent years in the U. S., not all markets have shared in the increase, and in some, margins have decreased. This has prompted some milk handlers to examine more closely what affects milk prices, marketing margins, and pricing practices.

  20. Characterization of Oxidative Stability of Fish Oil- and Plant Oil-Enriched Skimmed Milk

    DEFF Research Database (Denmark)

    Saga, Linda C.; Kristinova, Vera; Kirkhus, Bente;

    2013-01-01

    The objective of this research was to determine the oxidative stability of fish oil blended with crude plant oils rich in naturally occurring antioxidants, camelina oil and oat oil, respectively, in bulk and after supplementation of 1 wt% of oil blends to skimmed milk emulsions. Ability of crude...... oat oil and camelina oil to protect fish oil in bulk and as fish oil-enriched skimmed milk emulsions was evaluated. Results of oxidative stability of bulk oils and blends assessed by the Schaal oven weight gain test and by the rancimat method showed significant increase in oxidative stability when oat...... oil was added to fish oil in only 5 and 10 %, whereas no protective effect of camelina oil was observed when evaluated by these methods. Moreover, fish oil blended with oat oil conferred the lowest PV and lower amounts of volatile compounds during the storage period of 14 days at 4 °C. Surprisingly...

  1. Conditions of milk production of family farms located in the municipality of Alegre – ES, Brazil

    Directory of Open Access Journals (Sweden)

    Francisca Carvalho Nascimento Neta

    2015-12-01

    Full Text Available The present study aimed to assess the conditions and practices of production of raw milk used by farmers, as well as the conditions of storage and of the facilities and structures of the locations of the four collective cooling tanks, in the municipality of Alegre – ES. Data on sanitization procedures and physical structure of barns, source and treatment of water, hygiene practices and health status of milkers, udder cleaning and disinfection procedures and sanitary control of the herd was collected. The physical structure of the locations of the tanks was also assessed. The barns were ventilated, protected from moisture and had covers, and few had cemented floor and milking parlor. A low percentage of producers performed pre (21% and post (6% dipping procedures. Only 1/4 of the farmers used the mug test. All equipment and tools were washed after milking, but few farmers (5% used sanitizers and 94% of the farms used untreated water. Only 8% of the milkers had the habit of washing their hands before milking, and 9% of them were aware of IN 62 regulation of the Ministry of Agriculture, Livestock and Supply. None of the facilities had protection against the entrance of insects, rodents and environmental contaminants. They were poorly sized, did not allow the application of adequate sanitary measures and sanitary conditions were deficient. The high level of nonconformities regarding good manufacturing practices for milk production reinforced the need for the implementation of Good Agricultural Practices in rural farms for the purpose of obtaining milk produced according to the quality standards, preventing losses and ensuring the sustainability of dairy production in the region.

  2. 14 CFR 29.1013 - Oil tanks.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Oil tanks. 29.1013 Section 29.1013... STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Powerplant Oil System § 29.1013 Oil tanks. (a) Installation. Each oil tank installation must meet the requirements of § 29.967. (b) Expansion space. Oil tank...

  3. 14 CFR 25.1013 - Oil tanks.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Oil tanks. 25.1013 Section 25.1013... STANDARDS: TRANSPORT CATEGORY AIRPLANES Powerplant Oil System § 25.1013 Oil tanks. (a) Installation. Each oil tank installation must meet the requirements of § 25.967. (b) Expansion space. Oil tank...

  4. 27 CFR 24.167 - Tanks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Tanks. 24.167 Section 24... TREASURY LIQUORS WINE Construction and Equipment § 24.167 Tanks. (a) General. All tanks on wine premises... the intended purpose. Each tank used for wine operations will be located, constructed, and equipped...

  5. 7 CFR 58.320 - Brine tanks.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Brine tanks. 58.320 Section 58.320 Agriculture....320 Brine tanks. Brine tanks used for the treating of parchment liners shall be constructed of... liners. The tank should also be provided with a satisfactory drainage outlet....

  6. 14 CFR 27.1013 - Oil tanks.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Oil tanks. 27.1013 Section 27.1013... STANDARDS: NORMAL CATEGORY ROTORCRAFT Powerplant Oil System § 27.1013 Oil tanks. Each oil tank must be... space of not less than the greater of 10 percent of the tank capacity or 0.5 gallon, and where used...

  7. Milk metabolites and neurodegeneration: Is there crosstalk?

    Science.gov (United States)

    Thakur, Keshav; Anand, Akshay

    2015-10-01

    Milk has been considered as a natural source of nutrition for decades. Milk is known to be nutrient-rich which aids the growth and development of the human body. Milk contains both macro- and micronutrients. Breast milk is widely regarded as the optimal source of neonatal nutrition due to its composition of carbohydrates, proteins, minerals and antibodies. However, despite the wide use of milk products, investigations into the role of milk in degenerative diseases have been limited. This review will examine the relationship between the β-casein gene found in bovine milk and disease states by using age-related macular degeneration as an example. PMID:26526864

  8. Remediation of Hanford tank waste using magnetic separation

    International Nuclear Information System (INIS)

    Large volumes of high-level radioactive waste are stored at the Department of Energy's Hanford site. Magnetic separation, a physical separation, process, can be used to segregate actinides and certain fission products from the waste. High gradient magnetic separation (HGMS) tests have been performed successfully using a simulated, nonradioactive underground storage tank (UST) waste. Variations in HGMS test parameters included separator matrix material, magnetic field strength, slurry surfactant, and slurry solids loading. Cerium was added to the simulated tank waste to act as a uranium surrogate. Results show that over 77% of the uranium surrogate can be captured and concentrated from the original bulk with a simple procedure. The results of these tests and the feasibility of magnetic separation for pretreatment of UST waste are discussed

  9. Storage of Human Breast Milk

    Directory of Open Access Journals (Sweden)

    Gamze Can

    2007-10-01

    Full Text Available Storage of human breast milk by freezing or refrigeration of milk has been recommended especially at some social circumstances of most mothers who are regularly separated from their infants because of work. The greatest fear that has hindered the prospects of in - vitro storage of breast milk for any considerable period of time is the possibility of bacterial contamination and growth of infectious pathogens in the stored milk, there by rendering them unsafe for human consumption. The storage container can influence the cell content of milk, as the cells adhere to the walls of a glass container but not to polyethylene or polypropylene containers. Bacteriological examination of refrigerated milks has proven their safety for human consumption for even up to 72 h. For a storage over longer periods up to 1 month, freezing at - 20 0C could be recommended, but the most preferred method, especially for longer storage would be fresh freezing at - 70 0C, if affordable or available. The nutrient value of human milk is essentially unchanged, but the immunological properties are reduced by various storage techniques. Boiling and microwave radiation have not been recommended. [TAF Prev Med Bull 2007; 6(5.000: 375-379

  10. Technical note: variation in daily milk yield calculations for dairy cows milked in an automatic milking system.

    Science.gov (United States)

    Nielsen, P P; Pettersson, G; Svennersten-Sjaunja, K M; Norell, L

    2010-03-01

    An accurate estimation of the daily milk yield of dairy cows milked in an automatic milking system is not obvious because of variations in milking intervals and frequencies. Daily harvested milk varies substantially, and developing a method to be used for estimating daily milk production is of great importance. Three calculation methods (simple, semiadvanced, and advanced) were used. The simple method calculated rough daily milk production by summing up the yield per day. The semiadvanced used yield in combination with time since last milking to calculate the milk production per hour between milking; an average of the milk production per hour over the day was calculated and multiplied by 24. The advanced method calculated the milk production from midnight to midnight by using information about yield and time since last milking to calculate the exact milk production. The results show a clear preference for the advanced calculation method because the variation [variation for the advanced method=ln(1.79) for first lactation and ln(2.28) for later lactations] between days was reduced significantly (3 to 4 times lower compared with the simple method). Variation in daily harvested milk can be used as a management tool.

  11. Mare’s milk: composition and protein fraction in comparison with different milk species

    Directory of Open Access Journals (Sweden)

    Krešimir Kuterovac

    2011-06-01

    Full Text Available The usage of the mare’s milk as functional food especial for children intolerant to cow’s milk, with neurodermitis, allergies and similar disorders desiring to improve the quality of life is fiercely debated for last decades but there were no scientific studies to suggest such use of mare’s milk based on scientific research. The objectives of this study were to determine similarities of mare’s milk in comparison with milk of ruminants (cattle, sheep and goat and human milk in terms of milk composition and protein fraction as whey proteins, caseins and micelles size. All differences were discussed regarding usage of mare’s milk in human diet and compared to milk which is usually used in human nutrition. Regarding composition, the mare’s milk is similar to human milk in of crude protein, salt and lactose content, but it has significantly lower content of fat. Fractions of main proteins are similar between human and mare’s milk, except nitrogen casein (casein N which has twice lower content in human than in mare’s milk. Content of casein N from all ruminants’ milk differ much more. Just for true whey N and non-protein nitrogen (NPN similar content as human and mare’s milk has also goat milk. The casein content is the lowest in human milk; this content is three times greater in mare’s milk and six to seven times greater in goat’s and cow’s milk, while in sheep’s milk it is more than 10 times grater. In many components and fractions mare’s milk is more similar to human milk than milk of ruminants. A detail comparison of protein fraction shows quite large differences between milk of different species. More study and clinical research are needed that can recommend usage of mare’s milk in human diet as functional food on scientific bases.

  12. Underground Storage Tanks in Iowa

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — Underground storage tank (UST) sites which store petroleum in Iowa. Includes sites which have been reported to DNR, and have active or removed underground storage...

  13. Tank 48 Chemical Destruction - 13237

    Energy Technology Data Exchange (ETDEWEB)

    Simner, Steven P.; Aponte, Celia I.; Brass, Earl A. [Savannah River Remediation, LLC, Savannah River Remediation, Savannah River Site, Building 766-H, Aiken, SC 29808 (United States)

    2013-07-01

    Small tank copper-catalyzed peroxide oxidation (CCPO) is a potentially viable technology to facilitate the destruction of tetraphenylborate (TPB) organic solids contained within the Tank 48H waste at the Savannah River Site (SRS). A maturation strategy was created that identified a number of near-term development activities required to determine the viability of the CCPO process, and subsequent disposition of the CCPO effluent. Critical activities included laboratory scale validation of the process and identification of forward transfer paths for the CCPO effluent. The technical documentation and the successful application of the CCPO process on simulated Tank 48 waste confirm that the CCPO process is a viable process for the disposition of the Tank 48 contents. (authors)

  14. Milk ejection during automatic milking in dairy cows

    OpenAIRE

    Succi, G; Tamburini, A; A. Sandrucci; L. Bava

    2010-01-01

    Tactile stimulation (manual or mechanical) of the mammary gland causes alveolar milk ejection through a neuro-endocrine reflex arc (Dzidic et al., 2004). Oxytocin is the hormone that induces the contraction of the myoepitelial cells surrounding the alveoli.

  15. Modification of a liquid hydrogen tank for integrated refrigeration and storage

    Science.gov (United States)

    Swanger, A. M.; Jumper, K. M.; Fesmire, J. E.; Notardonato, W. U.

    2015-12-01

    The modification and outfitting of a 125,000-liter liquid hydrogen tank was performed to provide integrated refrigeration and storage capability. These functions include zero boil-off, liquefaction, and densification and therefore require provisions for sub-atmospheric tank pressures within the vacuum-jacketed, multilayer insulated tank. The primary structural modification was to add stiffening rings inside the inner vessel. The internal stiffening rings were designed, built, and installed per the ASME Boiler and Pressure Vessel Code, Section VIII, to prevent collapse in the case of vacuum jacket failure in combination with sub-atmospheric pressure within the tank. For the integrated refrigeration loop, a modular, skeleton-type heat exchanger, with refrigerant temperature instrumentation, was constructed using the stiffening rings as supports. To support the system thermal performance testing, three custom temperature rakes were designed and installed along the 21-meter length of the tank, once again using rings as supports. The temperature rakes included a total of 20 silicon diode temperature sensors mounted both vertically and radially to map the bulk liquid temperature within the tank. The tank modifications were successful and the system is now operational for the research and development of integrated refrigeration technology.

  16. Looking for a bulk point

    CERN Document Server

    Maldacena, Juan; Zhiboedov, Alexander

    2015-01-01

    We consider Lorentzian correlators of local operators. In perturbation theory, singularities occur when we can draw a position-space Landau diagram with null lines. In theories with gravity duals, we can also draw Landau diagrams in the bulk. We argue that certain singularities can arise only from bulk diagrams, not from boundary diagrams. As has been previously observed, these singularities are a clear diagnostic of bulk locality. We analyze some properties of these perturbative singularities and discuss their relation to the OPE and the dimensions of double-trace operators. In the exact nonperturbative theory, we expect no singularity at these locations. We prove this statement in 1+1 dimensions by CFT methods.

  17. CHOLESTEROL OXIDATION PRODUCTS IN MILK AND MILK PRODUCTS

    Directory of Open Access Journals (Sweden)

    A. Kemal SEÇKİN

    2004-02-01

    Full Text Available Cholesterol oxidation products (COPs are occurred by heat and light factors during processing, improper packaging and storage conditions. COPs are mutagenic, carcinogenity, cytotoxic, angiotoxic and damage to cell membrane and effect biosynthesis cholesterol in the metabolism . So, COPs have potential risk for public health. Also, in milk and milk products that have high cholesterol COPs can be also formed during processing and storage. Therefore it is necessary that measurements must be taken and standards must be in dairy about COPs.

  18. Bulk Viscosity of Interacting Hadrons

    OpenAIRE

    Wiranata, A.; M. Prakash

    2009-01-01

    We show that first approximations to the bulk viscosity $\\eta_v$ are expressible in terms of factors that depend on the sound speed $v_s$, the enthalpy, and the interaction (elastic and inelastic) cross section. The explicit dependence of $\\eta_v$ on the factor $(\\frac 13 - v_s^2)$ is demonstrated in the Chapman-Enskog approximation as well as the variational and relaxation time approaches. The interesting feature of bulk viscosity is that the dominant contributions at a given temperature ari...

  19. Bulk Viscosity of Interacting Hadrons

    CERN Document Server

    Wiranata, A

    2009-01-01

    We show that first approximations to the bulk viscosity $\\eta_v$ are expressible in terms of factors that depend on the sound speed $v_s$, the enthalpy, and the interaction (elastic and inelastic) cross section. The explicit dependence of $\\eta_v$ on the factor $(\\frac 13 - v_s^2)$ is demonstrated in the Chapman-Enskog approximation as well as the variational and relaxation time approaches. The interesting feature of bulk viscosity is that the dominant contributions at a given temperature arise from particles which are neither extremely nonrelativistic nor extremely relativistic. Numerical results for a model binary mixture are reported.

  20. Use of fluorometry for determination of skim milk powder adulteration in fresh milk

    Institute of Scientific and Technical Information of China (English)

    GUAN Rong-fa; LIU Dong-hong; YE Xing-qian; YANG Kai

    2005-01-01

    A FAST (fluorescence of advanced Maillard products and Soluble Tryptophan) method for identification of reconstituted milk made from skim milk powder in the fresh milk was developed. Considering milk and skim milk powders variations from different seasons and countries, milk was collected from different dairy farms in different seasons and skim milk powders were collected from different countries to measure the Tryptophan (Trp), advanced Maillard products (AMP) fluorescence values.The results showed that there were differences (P<0.01) between raw and reconstituted milk. The plot of values in each mixed level of raw and reconstituted milk had a correlation coefficient >0.97. The FAST method is a simple, rapid, low-cost and sensitive method enabling the detection of 5% reconstituted milk in fresh milk. The measurement of the Trp, AMP fluorescence values and calculation of the FAST index is a suitable method for large-scale monitoring of fresh milk samples.

  1. Economic viability of the third milking in systems of production using closed-circuit mechanical milking

    Directory of Open Access Journals (Sweden)

    Marcos Aurélio Lopes

    2014-08-01

    Full Text Available This study aimed to analyze the economic viability of the third milking in production systems using mechanical milking in a closed circuit, aiming to provide technicians and farmers with information to assist them in decision-making. Specifically, it intended: (a to estimate the cost of one milking; (b to estimate the cost of the third milking; (c to develop a mathematical equation to estimate the minimum amount of milk produced with two milkings, from which it would be economically feasible to do the third milking. Data were collected from three dairy farms, from November 2010 to March 2011, keeping a twice-a-day milking frequency, with three data collections in each farm, totalizing nine collections. Considering the average data, it would be feasible to do the third milking if the average milk yield per day of lactating cows in a twice-a-day milking frequency was greater than or equal to 24.43 kg of milk.

  2. Status report: Pretreatment chemistry evaluation FY1997 - Wash and leach factors for the single-shell tank waste inventory

    International Nuclear Information System (INIS)

    The wash factors will be used to partition the single-shell tank (SST) inventory into soluble and insoluble portions. The leach factors will be used to estimate the further removal of bulk analytes, such as chromium, aluminum, and phosphate, as well as minor components. Wash and leach factors are given here for 18 analytes, elements expected to drive the volume of material disposed of as high-level waste (HLW). These factors are determined by a weighting methodology developed earlier by this task. Tank-specific analyte inventory values depicted in Tank Waste Data Summary Worksheets, are calculated from concentrations obtained from characterization reports; the waste density; and the tank waste volume. The experimentally determined percentage of analytes removed by washing and leaching in a particular tank waste are translated into a mass (metric tons) in Experimental Washing and Leaching Data Summary Worksheets

  3. Technological suitability of goat milk for processing

    Directory of Open Access Journals (Sweden)

    Romualda Danków

    2011-06-01

    Full Text Available The world population of dairy goats is estimated at 300 million of which approximately 56% is reared in Asia. Goats raised in Europe (about 12 million constitute 4% of the world herd of these animals. The world goat milk production is assessed to be at the level of about 12 million tons and constitutes over 2% of the global milk obtained from different animal species. In many European countries, goat milk as a dairy raw material comes second after cow milk. The goat population in Poland is estimated at 190 000 heads. With regard to the content of basic constituents, goat milk is similar to cow milk, although it differs from it as to the qualitative composition of fat and protein. Species protein structure of goat milk, different from that of cow milk, causes fewer allergic reactions which are observed to occur in the case of intolerance to cow milk proteins. Rennin curd from goat milk is softer and less compact. A wide range of different products is manufactured from goat milk including: liquid milk (pasteurised and UHT, rennin and cottage cheeses, fermented beverages such as yoghurt, kefir, butter milk, cream, condensed milk, powdered milk, rice goats, butter and even chocolates “Goat’s Milk”.

  4. A COMPREHENSIVE TECHNICAL REVIEW OF THE DEMONSTRATION BULK VITRIFICATION SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    SCHAUS, P.S.

    2006-09-29

    In May 2006, CH2M Hill Hanford Group, Inc. chartered an Expert Review Panel (ERP) to review the current status of the Demonstration Bulk Vitrification System (DBVS). It is the consensus of the ERP that bulk vitrification is a technology that requires further development and evaluation to determine its potential for meeting the Hanford waste stabilization mission. No fatal flaws (issues that would jeopardize the overall DBVS mission that cannot be mitigated) were found, given the current state of the project. However, a number of technical issues were found that could significantly affect the project's ability to meet its overall mission as stated in the project ''Justification of Mission Need'' document, if not satisfactorily resolved. The ERP recognizes that the project has changed from an accelerated schedule demonstration project to a formally chartered project that must be in full compliance with DOE 413.3 requirements. The perspective of the ERP presented herein, is measured against the formally chartered project as stated in the approved Justification of Mission Need document. A justification of Mission Need document was approved in July 2006 which defined the objectives for the DBVS Project. In this document, DOE concluded that bulk vitrification is a viable technology that requires additional development to determine its potential applicability to treatment of a portion of the Hanford low activity waste. The DBVS mission need statement now includes the following primary objectives: (1) process approximately 190,000 gallons of Tank S-109 waste into fifty 100 metric ton boxes of vitrified product; (2) store and dispose of these boxes at Hanford's Integrated Disposal Facility (IDF); (3) evaluate the waste form characteristics; (4) gather pilot plant operability data, and (5) develop the overall life cycle system performance of bulk vitrification and produce a comparison of the bulk vitrification process to building a second LAW

  5. A COMPREHENSIVE TECHNICAL REVIEW OF THE DEMONSTRATION BULK VITRIFICATION SYSTEM

    International Nuclear Information System (INIS)

    In May 2006, CH2M Hill Hanford Group, Inc. chartered an Expert Review Panel (ERP) to review the current status of the Demonstration Bulk Vitrification System (DBVS). It is the consensus of the ERP that bulk vitrification is a technology that requires further development and evaluation to determine its potential for meeting the Hanford waste stabilization mission. No fatal flaws (issues that would jeopardize the overall DBVS mission that cannot be mitigated) were found, given the current state of the project. However, a number of technical issues were found that could significantly affect the project's ability to meet its overall mission as stated in the project ''Justification of Mission Need'' document, if not satisfactorily resolved. The ERP recognizes that the project has changed from an accelerated schedule demonstration project to a formally chartered project that must be in full compliance with DOE 413.3 requirements. The perspective of the ERP presented herein, is measured against the formally chartered project as stated in the approved Justification of Mission Need document. A justification of Mission Need document was approved in July 2006 which defined the objectives for the DBVS Project. In this document, DOE concluded that bulk vitrification is a viable technology that requires additional development to determine its potential applicability to treatment of a portion of the Hanford low activity waste. The DBVS mission need statement now includes the following primary objectives: (1) process approximately 190,000 gallons of Tank S-109 waste into fifty 100 metric ton boxes of vitrified product; (2) store and dispose of these boxes at Hanford's Integrated Disposal Facility (IDF); (3) evaluate the waste form characteristics; (4) gather pilot plant operability data, and (5) develop the overall life cycle system performance of bulk vitrification and produce a comparison of the bulk vitrification process to building a second LAW Immobilization facility or other

  6. Prediction of bovine milk true protein content by mid-infrared spectroscopy Estimativa do teor de proteína verdadeira do leite bovino por espectroscopia na região do infravermelho médio

    Directory of Open Access Journals (Sweden)

    Bruno Garcia Botaro

    2011-08-01

    Full Text Available The aim of this study was to estimate the concentration of milk true protein (TP by mid-infrared absorbance method (MIR in samples from bulk tank of dairy herds, and to determine the correlation between the results of TP of milk determined by Kjeldahl and MIR. Forty nine dairy herds were selected (17 Holstein, 6 Jersey and 26 Girolando for monthly collections of samples from bulk tanks during the period of one year (284 samples. Fat, lactose, crude protein and total solids were firstly determined by MIR, and then analyzed for total and true protein by Kjeldahl method. The regression equation to estimate TP contents based on MIR crude protein determination was as follows: TP=0.0021+(1.0104xCP, where: TP is the content of true protein, CP is the crude protein content determined by the MIR method, and 0.0155 is the model error term.O objetivo deste estudo foi estimar o teor de proteína verdadeira (PV do leite por meio de metodologia de espectroscopia na região do infravermelho médio (IVM, em amostras de tanque de rebanhos leiteiros comerciais, e determinar a correlação entre os resultados de proteína verdadeira do leite determinados pelo método de Kjeldahl e por IVM. Foram selecionados 49 rebanhos leiteiros (17 da raça Holandesa, seis da raça Jersey e 26 da raça Girolando para coletas mensais de amostras de leite de tanque durante o período de um ano, totalizando 284 amostras analisadas. As amostras de leite foram analisadas inicialmente em relação aos teores de gordura, lactose, proteína bruta e sólidos totais por IVM, sendo em seguida analisadas quanto ao teor de PB e PV pelo método de Kjeldahl. A equação de regressão para estimativa do teor de PV com base nos teores de proteína bruta foi a seguinte: PV=0,0021+(1,0104xPB; em que: PV é o teor de proteína verdadeira estimado; PB é o teor de proteína bruta pelo método IVM e 0,0155 é o erro apresentado pelo modelo.

  7. Plainview Milk Cooperative Ingredient Recall

    Data.gov (United States)

    U.S. Department of Health & Human Services — This list includes products subject to recall in the United States since June 2009 related to products manufactured by Plainview Milk Products Cooperative.

  8. Nonnutritive Sweeteners in Breast Milk.

    Science.gov (United States)

    Sylvetsky, Allison C; Gardner, Alexandra L; Bauman, Viviana; Blau, Jenny E; Garraffo, H Martin; Walter, Peter J; Rother, Kristina I

    2015-01-01

    Nonnutritive sweeteners (NNS), including saccharin, sucralose, aspartame, and acesulfame-potassium, are commonly consumed in the general population, and all except for saccharin are considered safe for use during pregnancy and lactation. Sucralose (Splenda) currently holds the majority of the NNS market share and is often combined with acesulfame-potassium in a wide variety of foods and beverages. To date, saccharin is the only NNS reported to be found in human breast milk after maternal consumption, while there is no apparent information on the other NNS. Breast milk samples were collected from 20 lactating volunteers, irrespective of their habitual NNS intake. Saccharin, sucralose, and acesulfame-potassium were present in 65% of participants' milk samples, whereas aspartame was not detected. These data indicate that NNS are frequently ingested by nursing infants, and thus prospective clinical studies are necessary to determine whether early NNS exposure via breast milk may have clinical implications. PMID:26267522

  9. CALCULATION OF DEMONSTRATION BULK VITRIFICATION SYSTEM MELTER INLEAKAGE AND OFF-GAS GENERATION RATE

    Energy Technology Data Exchange (ETDEWEB)

    MAY TH

    2008-04-16

    The River Protection Project (RPP) mission is to safely store, retrieve, treat, immobilize, and dispose of the Hanford Site tank waste. The Demonstration Bulk Vitrification System (DBVS) is a research and development project whose objective is to demonstrate the suitability of Bulk Vitrification treatment technology waste form for disposing of low-activity waste from the Tank Farms. The objective of this calculation is to determine the DBVS melter inleakage and off-gas generation rate based on full scale testing data from 38D. This calculation estimates the DBVS melter in leakage and gas generation rate based on test data. Inleakage is estimated before the melt was initiated, at one point during the melt, and at the end of the melt. Maximum gas generation rate is also estimated.

  10. ANNUAL RADIOACTIVE WASTE TANK INSPECTION PROGRAM 2009

    Energy Technology Data Exchange (ETDEWEB)

    West, B.; Waltz, R.

    2010-06-21

    Aqueous radioactive wastes from Savannah River Site (SRS) separations and vitrification processes are contained in large underground carbon steel tanks. Inspections made during 2009 to evaluate these vessels and other waste handling facilities along with evaluations based on data from previous inspections are the subject of this report. The 2009 inspection program revealed that the structural integrity and waste confinement capability of the Savannah River Site waste tanks were maintained. All inspections scheduled per LWO-LWE-2008-00423, HLW Tank Farm Inspection Plan for 2009, were completed. All Ultrasonic measurements (UT) performed in 2009 met the requirements of C-ESG-00006, In-Service Inspection Program for High Level Waste Tanks, Rev. 1, and WSRC-TR-2002-00061, Rev.4. UT inspections were performed on Tank 29 and the findings are documented in SRNL-STI-2009-00559, Tank Inspection NDE Results for Fiscal Year 2009, Waste Tank 29. Post chemical cleaning UT measurements were made in Tank 6 and the results are documented in SRNL-STI-2009-00560, Tank Inspection NDE Results Tank 6, Including Summary of Waste Removal Support Activities in Tanks 5 and 6. A total of 6669 photographs were made and 1276 visual and video inspections were performed during 2009. Twenty-Two new leaksites were identified in 2009. The locations of these leaksites are documented in C-ESR-G-00003, SRS High Level Waste Tank Leaksite Information, Rev.4. Fifteen leaksites at Tank 5 were documented during tank wall/annulus cleaning activities. Five leaksites at Tank 6 were documented during tank wall/annulus cleaning activities. Two new leaksites were identified at Tank 19 during waste removal activities. Previously documented leaksites were reactivated at Tanks 5 and 12 during waste removal activities. Also, a very small amount of additional leakage from a previously identified leaksite at Tank 14 was observed.

  11. Stabilization of in-tank residual wastes and external-tank soil contamination for the tank focus area, Hanford Tank Initiative: Applications to the AX tank farm

    International Nuclear Information System (INIS)

    This report investigates five technical areas for stabilization of decommissioned waste tanks and contaminated soils at the Hanford Site AX Farm. The investigations are part of a preliminary evacuation of end-state options for closure of the AX Tanks. The five technical areas investigated are: (1) emplacement of cementations grouts and/or other materials; (2) injection of chemicals into contaminated soils surrounding tanks (soil mixing); (3) emplacement of grout barriers under and around the tanks; (4) the explicit recognition that natural attenuation processes do occur; and (5) combined geochemical and hydrological modeling. Research topics are identified in support of key areas of technical uncertainty, in each of the five areas. Detailed cost-benefit analyses of the technologies are not provided. This investigation was conducted by Sandia National Laboratories, Albuquerque, New Mexico, during FY 1997 by tank Focus Area (EM-50) funding

  12. The Milk Supply Chain Project.

    OpenAIRE

    Smith, Howard; Thanassoulis, John

    2008-01-01

    This report presents the results of an Oxford University research project into the UK liquid milk supply chain. Financial sponsorship was kindly provided by the Milk Development Council (MDC) and by DEFRA. The research reported here is independent academic research which is not designed to further any group‟s particular agenda. We have striven to be as objective as possible. The project's aim is to investigate the competitive forces which influence the buyerseller relationsh...

  13. No Crying Over Spilt Milk

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A preliminary takeover plan to rescue beleaguered baby formula maker Sanlu is taking shape After months of waiting and specu-lation, the destiny of Sanlu GroupCo., the dairy products maker at the center of a scandal over chemi-cally contaminated milk, is starting to come into focus.Beijing-based Sanyuan Group isreportedly set to acquire Sanlu’s sevenkey affiliate milk plants in Shijiazhuang,capital of Hebei Province. Meanwhile,

  14. ROBOTIC TANK INSPECTION END EFFECTOR

    Energy Technology Data Exchange (ETDEWEB)

    Rachel Landry

    1999-10-01

    The objective of this contract between Oceaneering Space Systems (OSS) and the Department of Energy (DOE) was to provide a tool for the DOE to inspect the inside tank walls of underground radioactive waste storage tanks in their tank farms. Some of these tanks are suspected to have leaks, but the harsh nature of the environment within the tanks precludes human inspection of tank walls. As a result of these conditions only a few inspection methods can fulfill this task. Of the methods available, OSS chose to pursue Alternating Current Field Measurement (ACFM), because it does not require clean surfaces for inspection, nor any contact with the Surface being inspected, and introduces no extra by-products in the inspection process (no coupling fluids or residues are left behind). The tool produced by OSS is the Robotic Tank Inspection End Effector (RTIEE), which is initially deployed on the tip of the Light Duty Utility Arm (LDUA). The RTEE combines ACFM with a color video camera for both electromagnetic and visual inspection The complete package consists of an end effector, its corresponding electronics and software, and a user's manual to guide the operator through an inspection. The system has both coarse and fine inspection modes and allows the user to catalog defects and suspected areas of leakage in a database for further examination, which may lead to emptying the tank for repair, decommissioning, etc.. The following is an updated report to OSS document OSS-21100-7002, which was submitted in 1995. During the course of the contract, two related subtasks arose, the Wall and Coating Thickness Sensor and the Vacuum Scarifying and Sampling Tool Assembly. The first of these subtasks was intended to evaluate the corrosion and wall thinning of 55-gallon steel drums. The second was retrieved and characterized the waste material trapped inside the annulus region of the underground tanks on the DOE's tank farms. While these subtasks were derived from the original

  15. Bulk viscosity and deflationary universes

    CERN Document Server

    Lima, J A S; Waga, I

    2007-01-01

    We analyze the conditions that make possible the description of entropy generation in the new inflationary model by means of a nearequilibrium process. We show that there are situations in which the bulk viscosity cannot describe particle production during the coherent field oscillations phase.

  16. Longitudinal bulk acoustic mass sensor

    DEFF Research Database (Denmark)

    Hales, Jan Harry; Teva, Jordi; Boisen, Anja;

    2009-01-01

    A polycrystalline silicon longitudinal bulk acoustic cantilever is fabricated and operated in air at 51 MHz. A mass sensitivity of 100 Hz/fg (1 fg=10(-15) g) is obtained from the preliminary experiments where a minute mass is deposited on the device by means of focused ion beam. The total noise i...

  17. Selected welfare parameters of milk cows in relation to milking by a robot.

    OpenAIRE

    Průša, Jan

    2015-01-01

    The aim of this work was to gather and evaluate the evidence about the behaviour of dairy cows during the milking by a robot (including physiological reactions running after the milking). The following parameters were observed: number of milking per 24 hours, the time from onset to the robot and the teat cup deployment, the time between milking, the total time of milking, milk yield with each milking, average and total milk yield per day. The results were measured separately in heifers and in...

  18. Impact of the removal of chocolate milk from school milk programs for children in Saskatoon, Canada.

    Science.gov (United States)

    Henry, Carol; Whiting, Susan J; Phillips, Theodosia; Finch, Sarah L; Zello, Gordon A; Vatanparast, Hassan

    2015-03-01

    Studies in the United States report inclusion of flavoured milk in the diets of children and youth improves nutrient intakes. No research has investigated the contribution of flavoured milk to overall milk intake or the milk preferences of Canadian children. The objective of the study was to measure milk consumption (plain milk and flavoured milk) by children in an elementary school environment and investigate factors contributing to milk choice. A mixed-method research design was applied across 6 schools for 12 weeks. Milk waste was measured in grades 1-8 for 12 weeks. Weeks 1-4 (phase 1) and 9-12 (phase 3) provided both plain milk and flavoured milk as chocolate milk while weeks 5-8 (phase 2) provided plain milk only. Beverage Frequency Questionnaires were used in each phase (in grades 5-8 only) to assess usual beverage consumption. Statistical nutrient modelling was conducted to determine the effects of removing chocolate milk during phase 2 as a milk choice. Later, focus groups were conducted with students in grades 5-8 to determine what influences them to choose/not choose to drink milk. Total milk intake decreased by 12.3% when chocolate milk was removed from the schools (26.6% ± 5.2% to 14.31% ± 1.6%, p location (p = 0.035) and cost (p food/drink combinations.

  19. Numerical Investigation of Microgravity Tank Pressure Rise Due to Boiling

    Science.gov (United States)

    Hylton, Sonya; Ibrahim, Mounir; Kartuzova, Olga; Kassemi, Mohammad

    2015-01-01

    The ability to control self-pressurization in cryogenic storage tanks is essential for NASAs long-term space exploration missions. Predictions of the tank pressure rise in Space are needed in order to inform the microgravity design and optimization process. Due to the fact that natural convection is very weak in microgravity, heat leaks into the tank can create superheated regions in the liquid. The superheated regions can instigate microgravity boiling, giving rise to pressure spikes during self-pressurization. In this work, a CFD model is developed to predict the magnitude and duration of the microgravity pressure spikes. The model uses the Schrage equation to calculate the mass transfer, with a different accommodation coefficient for evaporation at the interface, condensation at the interface, and boiling in the bulk liquid. The implicit VOF model was used to account for the moving interface, with bounded second order time discretization. Validation of the models predictions was carried out using microgravity data from the Tank Pressure Control Experiment, which flew aboard the Space Shuttle Mission STS-52. Although this experiment was meant to study pressurization and pressure control, it underwent boiling during several tests. The pressure rise predicted by the CFD model compared well with the experimental data. The ZBOT microgravity experiment is scheduled to fly on February 2016 aboard the ISS. The CFD model was also used to perform simulations for setting parametric limits for the Zero-Boil-Off Tank (ZBOT) Experiments Test Matrix in an attempt to avoid boiling in the majority of the test runs that are aimed to study pressure increase rates during self-pressurization. *Supported in part by NASA ISS Physical Sciences Research Program, NASA HQ, USA

  20. CHARACTERIZATION AND ACTUAL WASTE TEST WITH TANK 5F SAMPLES

    Energy Technology Data Exchange (ETDEWEB)

    Hay, M. S.; Crapse, K. P.; Fink, S. D.; Pareizs, J. M.

    2007-08-30

    The initial phase of bulk waste removal operations was recently completed in Tank 5F. Video inspection of the tank indicates several mounds of sludge still remain in the tank. Additionally, a mound of white solids was observed under Riser 5. In support of chemical cleaning and heel removal programs, samples of the sludge and the mound of white solids were obtained from the tank for characterization and testing. A core sample of the sludge and Super Snapper sample of the white solids were characterized. A supernate dip sample from Tank 7F was also characterized. A portion of the sludge was used in two tank cleaning tests using oxalic acid at 50 C and 75 C. The filtered oxalic acid from the tank cleaning tests was subsequently neutralized by addition to a simulated Tank 7F supernate. Solids and liquid samples from the tank cleaning test and neutralization test were characterized. A separate report documents the results of the gas generation from the tank cleaning test using oxalic acid and Tank 5F sludge. The characterization results for the Tank 5F sludge sample (FTF-05-06-55) appear quite good with respect to the tight precision of the sample replicates, good results for the glass standards, and minimal contamination found in the blanks and glass standards. The aqua regia and sodium peroxide fusion data also show good agreement between the two dissolution methods. Iron dominates the sludge composition with other major contributors being uranium, manganese, nickel, sodium, aluminum, and silicon. The low sodium value for the sludge reflects the absence of supernate present in the sample due to the core sampler employed for obtaining the sample. The XRD and CSEM results for the Super Snapper salt sample (i.e., white solids) from Tank 5F (FTF-05-07-1) indicate the material contains hydrated sodium carbonate and bicarbonate salts along with some aluminum hydroxide. These compounds likely precipitated from the supernate in the tank. A solubility test showed the material