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Sample records for buckthorn hippophae rhamnoides

  1. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L. Seeds

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    Marie Sajfrtová

    2010-04-01

    Full Text Available Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L. seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40-80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.

  2. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

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    Sajfrtová, Marie; Ličková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk

    2010-01-01

    Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40–80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide. PMID:20480045

  3. Quality components of sea buckthorn (Hippophae rhamnoides) varieties.

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    Tiitinen, Katja M; Hakala, Mari A; Kallio, Heikki P

    2005-03-09

    The sensory quality and chemical constituents of juices from seven sea buckthorn (Hippophaerhamnoides L.) varieties were studied in two consecutive seasons. The juices were generally described as sour and astringent, with low sweetness and fruity flavor. The differences in sensory quality as well as in chemical composition between samples and years were significant (p acid (malic and quinic acids) from 3.1 to 5.1 g/100 mL, vitamin C from 29 to 176 mg/100 mL, and pulp oil from 0.7 to 3.6%. The soluble solids were between 7.4 and 12.6, the pH between 2.7 and 2.9, and the titrable acidity between 2.0 and 3.7. The redness was highest on Avgustinka and Raisa, but there were no differences in yellowness. Total sugar and the sugar/acid ratio correlated positively with sweetness and negatively with sourness and astringency, whereas total acid and titrable acidity correlated positively with sourness and astringency and negatively with sweetness.

  4. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L. Seed against Visible Light-Induced Retinal Degeneration in Vivo

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    Yong Wang

    2016-05-01

    Full Text Available Dietary proanthocyanidins (PACs as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L. seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6 and angiogenesis (VEGF levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  5. Data on coal dumps retrieving in petroşani basin using sea buckthorn (Hippophae rhamnoides

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    Andreea BRASOVAN

    2008-05-01

    Full Text Available This contribution presents data on the sea buckthorn (Hippöphae rhamnoides, with focus on its value on coal dumps retrieving in the Petroşani Basin. Due to its peculiar roots exposing a lot of nodules, this plant has an important role in fixing the atmospheric azoth and soil genesis. It is very adaptable to any kind of soil and has wide climatic exigencies, but is depending on light intensity. It has a large dissemination capacity, due to its light and numerous seeds. Besides its utilization on dump retrieving, sea buckthorn is used in medicine and pharmacy, as well as in organic fuel production.

  6. Hippophae rhamnoides

    Indian Academy of Sciences (India)

    Unknown

    2Institute of Soil and Water Conservation, Chinese Academy of Sciences and ... ability, unsteady yield and quality of introduced varieties, ... The 15 sea buckthorn cultivated varieties used in this study, ..... Assessment of genetic diversity using RAPD analysis in a ... huland Spiti using remote sensing and GIS (vol 80, pg 1107,.

  7. Effects of latitude and weather conditions on proanthocyanidins in berries of Finnish wild and cultivated sea buckthorn (Hippophaë rhamnoides L. ssp. rhamnoides).

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    Yang, Wei; Laaksonen, Oskar; Kallio, Heikki; Yang, Baoru

    2017-02-01

    Sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) of varieties 'Terhi' and 'Tytti' and one of wild origin were cultivated in southern and northern Finland, harvested during 2007-2013. Proanthocyanidins (PAs) were analyzed with HILIC UPLC-ESI-MS. The southern and northern samples were separated in the partial least squares discriminant analysis model (four factors, R(2) 0.75, Q(2) 0.70). The total PAs were more abundant in berries from the north (610-970mg/100gDW) than in those from the south (340-450mg/100gDW) (p<0.05). In northern Finland, the length of the growth season as well as the temperature sum and radiation sum of the growth season until harvest were negatively correlated with the total PAs in all the samples but positively with PA oligomers in 'Tytti' and 'Terhi'. In southern Finland no respective correlations were seen. 'Terhi' and 'Tytti' had different trends in the content of total PA and oligomers in overripe stages. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. HPLC-DAD-MS/MS profiling of antioxidant flavonoid glycosides in sea buckthorn (Hippophae rhamnoides L.) seeds.

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    Arimboor, Ranjith; Arumughan, C

    2012-09-01

    This study was aimed at the chemical profiling of flavonoid glycosides in antioxidant (AO) fractions of sea buckthorn (Hippophae rhamnoides) seed. Seed fractions were evaluated for their DPPH, ABTS, superoxide and hydroxyl radical scavenging, ferric reduction, ferrous chelation and xanthine oxidase inhibitory capacities. HPLC-DAD-ESI-MS/MS analytical conditions for the profiling of seed flavonoids were optimized and the AO-rich fraction was analysed. Quercetin-3-O-rutinoside (5.9%), isorhamnetin-3-O-rutinoside (4.9%) and isorhamnetin-3-O-sophroside-7-O-rhamnoside (3.7%) were found as the major flavonoid glycosides in the fraction. Significant amounts of isorhamnetin-3-O-glucoside (2.8%), 3-O-sophroside-7-O-rhamnosides of quercetin (2.4%) and kaempherol (1.3%), and 3-O-glucoside-7-O-rhamnosides of quercetin (1.1%) and isorhamnetin (1.1%) along with their free forms: isorhamnetin (2.7%), quercetin (1.1%) and kaempherol (0.6%) were also found in the fraction. The identification of flavonoids as the major less polar AO phenolics in the seeds was rationalized by demonstrating the high AO activity of isorhamnetin, quercetin, kaempherol and quercetin-3-O-rutinoside.

  9. Chemical constituents of sea buckthorn (Hippophae rhamnoides L. fruit in populations of central Alborz Mountains in Iran

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    A. Kuhkheil

    2017-07-01

    Full Text Available Background and objectives: Hippophae rhamnoides L. known as sea buckthorn is a deciduous medicinal shrub belonging to Elaeagnaceae family. In this study, the most important chemical constituents of sea buckthornwere evaluated in wild populations of central Alborz Mountains in Iran during the growth season of 2014 and 2015. Methods: Phytochemical analysis of fruit pulp and seed oil traits was performed using different methods of chromatography such as spectrophotometry, HPLC and GC. Results: Based on the results of combined analysis of variance, significant (p≤0.01 difference ranges between populations were found in respect to fruit dry weight (21.32 to 32.03%, total phenolic compounds (20.78 to 34.60 mg/g, extractable tannin (1.99 to 5.74 mg/g, glucose (38.14 to 110.70 mg/g, total carotenoids (0.80 to 1.17 mg/g, lycopene (0.13 to 0.20 mg/g, β-carotene (0.18 to 0.26 mg/g, total flavonoids (0.98 to 2.80 mg/g, total soluble solids (TSS (11.85 to 31.50%, vitamin C (1.47 to 8.96 mg/g, seed oil content (4.51 to 7.91%, and two major unsaturated fatty acids including linoleic acid (28.71 to 37.44% and linolenic acid (21.52 to 28.28%. Factor analysis based on principal component analysis (PCA revealed most important traits with the highest correlation factor such as vitamin C, carbohydrates, TSS, fruit dry weight (FDW, and tannin for the first component. Conclusion: content of vitamin C was the main variable in chemical constituents for effective detection of original wild populations of central Alborz Mountains. Accordingly, sea buckthorn populations were divided into four main clusters and groups with high diversity based on their chemical compositions.

  10. Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides, Apricot Pulp and Apricot Kernel (Prunus armeniaca

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    Elena Andreea Pop

    2015-11-01

    Full Text Available A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L. and apricot fruits (Prunus armeniaca L. are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits. The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC  method.  Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection. The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg than in apricot fruits (1.09 mg/kg while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg. Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.

  11. Structural investigations of flavonol glycosides from sea buckthorn (Hippophaë rhamnoides) pomace by NMR spectroscopy and HPLC-ESI-MS(n).

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    Rösch, Daniel; Krumbein, Angelika; Mügge, Clemens; Kroh, Lothar W

    2004-06-30

    Four flavonol glycosides were isolated from an extract of sea buckthorn pomace (Hippophaë rhamnoides) by Sephadex LH-20 gel chromatography and semipreparative HPLC. Their structures were elucidated by hydrolysis studies, ESI-MS(n), UV, and (1)H and (13)C NMR spectroscopy. The occurrence of the major flavonol glycoside kaempferol 3-O-beta-sophoroside-7-O-alpha-rhamnoside in sea buckthorn is described here for the first time. A further 21 flavonol glycosides of Sephadex LH-20 fractions of sea buckthorn pomace were characterized by HPLC-DAD-ESI-MS. The characteristic MS-MS and MS(3) fragmentation pattern of flavonol glycosides previously identified in sea buckthorn juice and of flavonol glycosides identified by NMR spectroscopy gave valuable indications for their identification. The results demonstrate that loss of the sugar moiety from C-7 of the aglycon is more favored than fission of the glycosidic linkage at the C-3 position. Thus, most of the compounds identified were 7-rhamnosides of isorhamnetin, kaempferol, and quercetin, which exhibit different substitution patterns at the C-3 position, mainly glucosides, rutinosides, and sophorosides. In addition, numerous flavonol glycosides were detected lacking a sugar moiety at C-7. Finally, eight flavonol derivatives were identified that are acylated by hydroxybenzoic or hydoxycinnamic acids.

  12. Beta-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

    Czech Academy of Sciences Publication Activity Database

    Sajfrtová, Marie; Ličková, I.; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk

    2010-01-01

    Roč. 11, č. 4 (2010), s. 1842-1850 E-ISSN 1422-0067 R&D Projects: GA MŠk 2B06024 Institutional research plan: CEZ:AV0Z40720504; CEZ:AV0Z50380511 Keywords : sea buckthorn seed * supercritical carbon dioxide * beta-sitosterol Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 2.279, year: 2010

  13. Effect of monospecific and mixed sea-buckthorn (Hippophae rhamnoides plantations on the structure and activity of soil microbial communities.

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    Xuan Yu

    Full Text Available This study aims to evaluate the effect of different afforestation models on soil microbial composition in the Loess Plateau in China. In particular, we determined soil physicochemical properties, enzyme activities, and microbial community structures in the top 0 cm to 10 cm soil underneath a pure Hippophae rhamnoides (SS stand and three mixed stands, namely, H. rhamnoides and Robinia pseucdoacacia (SC, H. rhamnoides and Pinus tabulaeformis (SY, and H. rhamnoides and Platycladus orientalis (SB. Results showed that total organic carbon (TOC, total nitrogen, and ammonium (NH4(+ contents were higher in SY and SB than in SS. The total microbial biomass, bacterial biomass, and Gram+ biomass of the three mixed stands were significantly higher than those of the pure stand. However, no significant difference was found in fungal biomass. Correlation analysis suggested that soil microbial communities are significantly and positively correlated with some chemical parameters of soil, such as TOC, total phosphorus, total potassium, available phosphorus, NH4(+ content, nitrate content (NH3(-, and the enzyme activities of urease, peroxidase, and phosphatase. Principal component analysis showed that the microbial community structures of SB and SS could clearly be discriminated from each other and from the others, whereas SY and SC were similar. In conclusion, tree species indirectly but significantly affect soil microbial communities and enzyme activities through soil physicochemical properties. In addition, mixing P. tabulaeformis or P. orientalis in H. rhamnoides plantations is a suitable afforestation model in the Loess Plateau, because of significant positive effects on soil nutrient conditions, microbial community, and enzyme activities over pure plantations.

  14. Effects of temperature sum on vitamin C concentration and yield of sea buckthorn (Hippophae rhamnoides fruit: optimal time of fruit harvest

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    Yingmou Yao

    1993-12-01

    Full Text Available To investigate the effects of temperature sum on vitamin C concentration (Vc, yield and maturity of sea buckthorn fruit (Hippophae rhamnoides L. and to predict the optimal harvest time, berries were collected from eight genotypes at an interval of about one week from August 16 to December 2. Maturity was visually observed, berry weight measured and Vc determined. Berries matured at 1165-1316 degree-days (d.d.. Vc reached maximum at about 1229 d.d., while fruit size and yield reached maximum at 1380 d.d.. Mathematical models of polynomial equations were highly significant for predicting the effects of temperature sum on Vc, maturity and fruit yield. Optimal harvest time for maximizing Vc, yield or economic income could be determined according to differential equations. Great variations in Vc, fruit maturity and fruit size suggested good opportunities for selection and breeding. Low rank correlations in vitamin C concentration during fruit maturity, however, call for special attention in selection and breeding.

  15. Determination of optimum harvesting time for vitamin C, oil and mineral elements in berries sea buckthorn (hippophae rhamnoides)

    International Nuclear Information System (INIS)

    Arif, S.; Ahmed, S.D.; Shah, A.H.; Hassan, L.; Awan, S.I.; Hamid, A.; Batool, F.

    2010-01-01

    Sea buck thorn a magic plant from Northern areas of Pakistan has multiple uses against various ailments, soil enrichment and environmental purposes. The fruit berries are rich in vitamin C, vitamin A, vitamin E, essential oil, Phytosterol and minerals (Fe, Ca, P, Mn and K). The micro nutrient like vitamin C, oil, phosphorus, magnesium and calcium contents in fruit berries of cultivated sea buck thorn (Hippophae rhamnoides. L. spp. sinensis) harvested at three ripening times were determined using biochemical analysis techniques. Harvesting at different stages of fruit ripening was the primary factor determining maximum expression of these biochemical constituents. Biochemical contents were determined at three fruit developmental stages i.e., unripened stage, medium stage and at full-ripened stage. During this study a decline in vitamin C contents was observed along with the fruit ripening. The oil contents in both seed and pulp increased with fruit ripening. Similarly, the mineral contents like magnesium, calcium and phosphorus contents increases with the fruit ripening in sea buck thorn. The main idea was to identify the maximum expression of biochemical at different stages of fruit maturity. It is concluded that it is better to harvest fruit berries at medium stage of fruit ripening when maximum vitamin C is present. For maximum oil and mineral contents fruit must be harvested at ripening stage. The fruit mesocarp is the area where all genes related with micro nutrients are active at one time i.e., when fruit is maturing, hence the characterization of gene expression activities at this stage may help in the isolation of these genes for future commercial use. (author)

  16. Thermal stability of the complex formed between carotenoids from sea buckthorn (Hippophae rhamnoides L.) and bovine β-lactoglobulin

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    Aprodu, Iuliana; Ursache, Florentina-Mihaela; Turturică, Mihaela; Râpeanu, Gabriela; Stănciuc, Nicoleta

    2017-02-01

    Sea buckthorn has gained importance as a versatile nutraceutical, due to its high nutritive value in terms of carotenoids content. β-Lactoglobulin (β-LG) is a natural carrier for various bioactive compounds. In this study, the effect of thermal treatment in the temperature range of 25 to 100 °C for 15 min on the complex formed by β-LG and carotenoids from sea buckthorn was reported, based on fluorescence spectroscopy, molecular docking and molecular dynamics simulation results. Also, the berries extracts were analyzed for their carotenoids content. The chromatographic profile of the sea buckthorn extracts revealed the presence of zeaxanthin and β-carotene, as major compounds. The Stern-Volmer constants and binding parameters between β-LG and β-carotene were estimated based on quenching experiments. When thermally treating the β-LG-carotenoids mixtures, an increase in intrinsic and extrinsic fluorescence intensity up to 90 °C was observed, together with blue-shifts in maximum emission in the lower temperature range and red-shifts at higher temperature. Based on fluorescence spectroscopy results, the unfolding of the protein molecules at high temperature was suggested. Detailed information obtained at atomic level revealed that events taking place in the complex heated at high temperature caused important changes in the β-carotene binding site, therefore leading to a more thermodynamically stable assembly. This study can be used to understand the changes occurring at molecular level that could help food operators to design new ingredients and functional foods, and to optimize the processing methods in order to obtain healthier food products.

  17. Phytochemical composition and antibacterial activity of the essential oils from different parts of sea buckthorn (Hippophae rhamnoides L.).

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    Yue, Xuan-Feng; Shang, Xiao; Zhang, Zhi-Juan; Zhang, Yan-Ni

    2017-04-01

    Essential oils from the seed, pulp, and leaf of sea buckthorn were obtained with hydrodistillation, and their phytochemical composition was analyzed through gas chromatography-mass spectrometry. Furthermore, the antibacterial activity of the oils was tested on five food-borne bacteria by spectrometry and evaluated in terms of minimum inhibitory concentration. The results indicate that the composition of all essential oils is dominated by free fatty acids, esters, and alkanes. Minimum inhibitory concentration values on each bacterium were obtained for oils from different parts. The oils from different parts exhibited nearly equal inhibitory effect on Staphylococcus aureus. The pulp oil was found to be the most effective for the rest of bacteria tested except Escherichia coli, on which seed oil shows twice the inhibitory effect to that of leaf or pulp oil. Three natural inhibitory examples were found comparable with or even better than the positive control: pulp oil on Bacillus subtilis, and pulp oil and leaf oil on Bacillus coagulans. Copyright © 2016. Published by Elsevier B.V.

  18. Phytochemical composition and antibacterial activity of the essential oils from different parts of sea buckthorn (Hippophae rhamnoides L.

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    Xuan-Feng Yue

    2017-04-01

    Full Text Available Essential oils from the seed, pulp, and leaf of sea buckthorn were obtained with hydrodistillation, and their phytochemical composition was analyzed through gas chromatography–mass spectrometry. Furthermore, the antibacterial activity of the oils was tested on five food-borne bacteria by spectrometry and evaluated in terms of minimum inhibitory concentration. The results indicate that the composition of all essential oils is dominated by free fatty acids, esters, and alkanes. Minimum inhibitory concentration values on each bacterium were obtained for oils from different parts. The oils from different parts exhibited nearly equal inhibitory effect on Staphylococcus aureus. The pulp oil was found to be the most effective for the rest of bacteria tested except Escherichia coli, on which seed oil shows twice the inhibitory effect to that of leaf or pulp oil. Three natural inhibitory examples were found comparable with or even better than the positive control: pulp oil on Bacillus subtilis, and pulp oil and leaf oil on Bacillus coagulans.

  19. Use of electron beam irradiation to improve the microbiological safety of Hippophae rhamnoides

    Energy Technology Data Exchange (ETDEWEB)

    Minea, R. [National Institute for Lasers, Plasma and Radiation Physics, Electron Accelerators Department, 409 Atomistilor St., Bucharest-Magurele 077125 (Romania); Nemtanu, M.R. [National Institute for Lasers, Plasma and Radiation Physics, Electron Accelerators Department, 409 Atomistilor St., Bucharest-Magurele 077125 (Romania)], E-mail: monica.nemtanu@inflpr.ro; Manea, S.; Mazilu, E. [S.C. Hofigal Export-Import S.A., 2A Intrarea Serelor St., 75669, Bucharest 4 (Romania)

    2007-09-21

    Sea buckthorn (Hippophae rhamnoides) is increasingly used in food supplements due to its dietary and medicinal compounds with a beneficial role in human diet and health. As many other medicinal plants, sea buckthorn can be contaminated with microorganisms which exerts an important impact on the overall quality of the products. Irradiation is an effective method for food preservation because it is able to destroy pathogenic microorganisms keeping the organoleptic and nutritional characteristics of the foods. The objective of the present study was to investigate the application of electron beam irradiation in order to improve the microbiological safety of sea buckthorn. The experimental results indicated that the electron beam treatment might be a good method to remove undesirable microorganisms from sea buckthorn without significant changes in its active principles.

  20. Protective effect of Hippophae rhamnoides leaf extract on gamma irradiation induced clastogenecity in mice

    International Nuclear Information System (INIS)

    Tyagi, Anuradha; Prasad, Jagdish; Bala, Madhu

    2012-01-01

    Hippophae rhamnoides (sea buckthorn) is a plant belonging to Elaeagnaceae family and is distributed worldwide. It has variety of uses from nutritional food to pharmacological application. The study was aimed to analyse the extract from Hippophae rhamnoides leaves for their possible protective effects against the whole body 60 Co-a-irradiation. The study was performed on six groups of male mice i.e. untreated group, H. rhamnoides extract group, irradiated (2Gy), irradiated (3Gy), H. rhamnoides and irradiated (2Gy) and H. rhamnoides and irradiated (3Gy). In each group micronucleus test was performed utilising bone marrow and peripheral blood. The mice were sacrificed 30 hrs after treatment and analysed for the presence of micronuclei. In the present study, there was no significant increase in the frequency of either micronucleated polychromatic erythrocytes (MNPCEs) or normochromatic erythrocytes (NCE) in H. rhamnoides extract treated group over the negative control group of animals, indicating its non-clastogenic and non-toxic activity in the erythropoietic system. H. rhamnoides extract showed good anti-clastogenic activity against the a-irradiation induced clastogenecity in both the tissues i.e. bone marrow and peripheral blood by reducing the frequency of micronuclei. Also the administration of H. rhamnoides extract along with irradiation was slightly able to increase the frequency of PCE in bone marrow as well as in peripheral blood in comparison to the irradiated group (2Gy and 3Gy) indicating its ability to reduce the toxicity caused by irradiation in the erythropoietic system. Thus the results indicate the non-clastogenic effect of H. rhamnoides leaf extract and significant protective activity against 60 Co-a-irradiation suggesting its pharmacological significance for development of radioprotector. (author)

  1. Variability in Foliar Ellagitannins of Hippophaë rhamnoides L. and Identification of a New Ellagitannin, Hippophaenin C.

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    Suvanto, Jussi; Tähtinen, Petri; Valkamaa, Saku; Engström, Marica T; Karonen, Maarit; Salminen, Juha-Pekka

    2018-01-24

    Berries of common sea-buckthorn (Hippophaë rhamnoides L.) are well-known and used for their bioactive components, and while there is a considerable amount of research on the leaves as well, their ellagitannins (ETs) have not been a prominent focus of research. We identified and quantified ten major hydrophilic polyphenols, all ETs, in H. rhamnoides leaves and compared their abundance between 58 plant individuals. Of these compounds, hippophaenin C was characterized as a new ellagitannin by various spectrometric methods. The total concentrations of ETs ranged from 42.5 mg g -1 dry weight (DW) to 109.1 mg g -1 DW between individual plants. Among the ETs, hippophaenin C, stachyurin, and casuarinin were on average the most abundant compounds. Sexes did not differ significantly, while cultivars showed variation in some ETs. These results suggest that H. rhamnoides leaves could be a potential and rich source of several ETs.

  2. Leaves of Hippophae rhamnoides prevent taste aversion in gamma-irradiated rats.

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    Gupta, Vanita; Bala, Madhu; Prasad, Jagdish; Singh, Surinder; Gupta, Manish

    2011-12-01

    Hippophae rhamnoides (Sea buckthorn), a traditionally known plant for nutritional and therapeutic values, is under active investigation for radioprotective properties. This study investigated effects of aqueous leaf extract from H. rhamnoides on (60)Co-γ-radiation induced changes in behavior, oxidative stress and serotonin levels in jejunum and plasma of rats. Conditioned taste aversion (CTA) was chosen as the assay to record behavioral changes and was assessed in terms of saccharine preference ratio (SPR). Whole body (60)Co-γ-irradiation (2 Gy) induced significant nonrecoverable CTA (25.6 ± 3.6% SPR, t(6) = 3.499, p loss in body weight (b.w.). One time treatment with leaf extract before irradiation, countered radiation induced CTA and loss in body weight. The 12 mg/kg b.w. concentration of leaf extract caused complete extinction of CTA [100.3 ± 6.4% SPR, t(6) = 5.879, p < .01] after day 3 and the effect was significantly higher than positive control, Ondansetrone (70.0 ± 8.9% SPR). Treatment with leaf extract before irradiation significantly countered radiation induced (1) decrease in antioxidant protection, (2) increase in levels of corticosterone (CS) in plasma, (3) increase in levels of serotonin in jejunum and plasma. Present investigation demonstrated that H. rhamnoides leaf extract prevented behavioral changes induced at clinical radiation doses. Hippophae leaves are nontoxic and are being consumed as tea and other beverages. CTA in rats is a considered parallel process to nausea and vomiting in human beings. These findings, put together, suggest that dietary supplements from Hippophae leaves could be developed for preventing behavioral changes in subjects exposed to radiation.

  3. Rhizospheric fungi and their link with the nitrogen-fixing Frankia harbored in host plant Hippophae rhamnoides L.

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    Zhou, Xue; Tian, Lei; Zhang, Jianfeng; Ma, Lina; Li, Xiujun; Tian, Chunjie

    2017-12-01

    Sea buckthorn (Hippophae rhamnoides L.) is a pioneer plant used for land reclamation and an appropriate material for studying the interactions of symbiotic microorganisms because of its nitrogen-fixing root nodules and mycorrhiza. We used high-throughput sequencing to reveal the diversities and community structures of rhizospheric fungi and their link with nitrogen-fixing Frankia harbored in sea buckthorn collected along an altitude gradient from the Qinghai Tibet Plateau to interior areas. We found that the fungal diversities and compositions varied between different sites. Ascomycota, Basidiomycota, and Zygomycota were the dominant phyla. The distribution of sea buckthorn rhizospheric fungi was driven by both environmental factors and the geographic distance. Among all examined soil characteristics, altitude, AP, and pH were found to have significant (p < 0.05) effect on the rhizospheric fungal community. The rhizospheric fungal communities became more distinct as the distance increased. Moreover, co-inertia analysis identified significant co-structures between Frankia and AMF communities in the rhizosphere of sea buckthorn. We conclude that at the large scale, there are certain linkages between nitrogen-fixing bacteria and the AMF expressed in the distributional pattern. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. The Protective Effect of Hippophae Rhamnoides Carotenoid Extract Against Lipid Peroxidation in Crude Vegetable Oils

    Directory of Open Access Journals (Sweden)

    Sanda Andrei

    2014-11-01

    Full Text Available Vegetable oils are important elements of the human diet because they contain essential nutritional factors. Due to the manufacturing processes or inadequate conditions of storage, they may also contain lipid oxidation products that are toxic to the body. The purpose of this paper is to test the protective effect of carotenoid-rich extracts obtained from the fruits of Hippophae rhamnoides on crude sunflower, pumpkin and olive oils oxidative processes. In order to evaluate the effect of antioxidant carotenoids, three stages were followed: thermal induction of lipid peroxidation in the presence of AAPH (2,2'-Azobis(2-amidinopropane dihydrochloride; determination of the level of lipid peroxidation in oxidized oils in the presence and absence of antioxidants, by quantifying the concentration of conjugated dienes and malonyl dialdehyde (MDA; determination of the level of lipid peroxidation by evaluating the profile of the fatty acids and the ratio between the saturated and unsaturated fatty acids (UFA / SFA, using an GC-MS method. In the case of sunflower oil, it was observed that sea buckthorn fruit extract significantly decreased MDA concentration but does not significantly reduce the concentration of conjugated dienes. The protective effect of carotenoids is more evident in the case of oil from pumpkin seeds. In the olive oil, unlike the first two types of oils, the carotenoids extract inhibits both the MDA and the conjugated dienes formation to a lesser extent, statistically insignificant. Overall, the ratio UFA / SFA decreases in crude oxidized oils. In the oils in which carotenoids were added was observed an increase in the UFA / SFA ratio. Carotenoids fraction from sea buckthorn fruits, rich in xanthophylls’ esters, possess a good antioxidant effect, protecting vegetable oils against peroxidation processes induced in the presence of AAPH

  5. Genomics in development of radiation countermeasure from Hippophae rhamnoides

    International Nuclear Information System (INIS)

    Madhu Bala

    2013-01-01

    Development of an effective and non-toxic radiation countermeasure has been a global challenge, till date. On one hand single molecules have failed due to unacceptable toxicity and on the other hand herbal drugs, though non-toxic, are generally neglected because of issues such as incomplete understanding on mechanisms of actions, efficacy and quality control. In the conventional drug discovery process, a single active constituent is isolated, purified, and standardized, but herbal drugs have complex composition and multiple targeted actions. The assessment of efficacy and toxicity of herbal drugs is, therefore, not as straight forward as in case of single active compound. The completion of genome projects of human, mouse and many other simpler experimental models has opened multiple newer avenues for evaluating the metabolism, safety and efficacy of herbal drugs. The radioprotective properties of leaves of Hippophae rhamnoides L. (common name Seabuckthorn, family Eleagnaceae) were reported (Bala et al., 2009, J Herbs and Med Plants). Administration of SBL-1 (a coded herbal preparation from Seabuckthorn leaves), before whole body exposure to lethal dose of 60 Co-gamma-rays (10 Gy), rendered >90% survivors in mice population, while 100% mortality was observed in non-SBL-1 treated, 60 Co-gamma- irradiated (10 Gy) controls. Though, plant H. rhamnoides is popular for its medicinal and neutraceutical values and finds place is in many traditional medicinal systems world over, its prophylactic use as a radiation countermeasure is novel and needed thorough evaluation. To gain in depth understanding on metabolism and safety of SBL-1, the experimental animals were treated with radioprotective concentration of SBL-1. The genome evaluation was performed at different time intervals after the treatment. This talk highlights some of the prominent molecular pathways and genes affected by SBL-1 treatment in experimental animals. Our study suggested that application of genomics

  6. Colorimetric Models Used for Establishing the Optimal Dose for Sterilizing the Sea Buck thorn (Hippophae rhamnoides) Leaves Powder with Ionizing Radiation

    International Nuclear Information System (INIS)

    Minea, R.; Popescu, M.I.; Dumitrascu, M.; Sima, E.; Mitru, E.; Manea, St.; Mazilu, S.

    2009-01-01

    The current work intends to promote the development of the methods for establishing the degree of sterilization with ionizing radiations of the herbs in order to use them for obtaining food supplements, but also to obtaining medicines and cosmetic products. Obtaining the raw material, Sea Buckthorn (Hippophae rhamnoides) leaves, at a high level of sterilization without influencing the quality of active principles, is a desideratum of the economic agents in the area. The employed colorimetric methods have practically no impact on the studied herbs and, in the same time, they provide complex information on the effect of the ionizing radiations. The colorimetric methods allow to develop some models to be used both in the indirect evaluation of the microbial charge, before and after the treatment with ionizing radiations and for establishing the optimal necessary dose. The trials were made on Sea Buckthorn (Hippophae rhamnoides) leaves powder supplied by S.C. HOFIGAL EXPORT-IMPORT S.A. from Bucharest. The validation of the obtained colorimetric model was made through some spectrometric setups from the UV-Vis area, on the contents in: polyphenol carboxylic acids, flavones derivates, redox enzymes of superoxide dismutase type, and in the antioxidant activity

  7. Growth and physiological responses to drought and elevated ultraviolet-B in two contrasting populations of Hippophae rhamnoides

    Energy Technology Data Exchange (ETDEWEB)

    Yongqing Yang; Yinan Yao; Gang Xu [Chinese Academy of Sciences, Chengdu Inst. of Biology, Chengdu (China); Chunyang Li [Graduate School of the Chinese Academy of Sciences, Beijing (China)

    2005-08-01

    In the southeast of the Qinghai-Tibetan Plateau of China, sea buckthorn (Hippophae rhamnoides L.), which is a thorny nitrogen-fixing deciduously perennial shrub, has been widely used in forest restoration as the pioneer species. In our study, two contrasting populations from the low and high altitudinal regions were employed to investigate the effects of drought, ultraviolet-B (UV-B) and their combination on sea buckthorn. The experimental design included two watering regimes (well watered and drought stressed) and two levels of UV-B (with and without UV-B supplementation). Drought significantly decreased total biomass, total leaf area and specific leaf area (SLA), and increased root/shoot ratio, fine root/coarse root ratio and abscisic acid content (ABA) in both populations. However, the high altitudinal population was more responsive to drought than the low altitudinal population. On the other hand, elevated UV-B induced increase in anthocyanins in both populations, whereas he accumulation of UV-absorbing compounds occurred only in the low altitudinal population. The drought-induced enhancement of ABA in the high altitudinal population was significantly suppressed in the combination of drought and elevated UV-B. Moreover, significant drought x UV-B interaction was detected on total biomass in both populations, total leaf area and fine root/coarse root in the low altitudinal population, and SLA in the high altitudinal population. These results demonstrated that there were different adaptive responses between two contrasting populations, the high altitudinal population exhibited higher tolerance to drought and UV-B than the low altitudinal population. (au)

  8. Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma

    Directory of Open Access Journals (Sweden)

    Beata Olas

    2016-01-01

    Full Text Available Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation and the generation of superoxide anion (O2-∙ in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2 or H2O2/Fe (a donor of hydroxyl radicals. The tested fraction of H. rhamnoides (0.5– 50 µg/mL; the incubation time: 15 and 60 min inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2-∙ in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL. The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

  9. The Anticancer Activity of Sea Buckthorn [Elaeagnus rhamnoides (L. A. Nelson

    Directory of Open Access Journals (Sweden)

    Beata Olas

    2018-03-01

    Full Text Available Various parts of sea buckthorn [Elaeagnus rhamnoides (L. A. Nelson], particularly the berries, known also as seaberries, or Siberian pineapples, are characterized by a unique composition of bioactive compounds: phenolic compounds, vitamins (especially vitamin C, unsaturated fatty acids, and phytosterols such as beta-sitosterol. These berries, together with the juices, jams, and oils made from them, have a range of beneficial antioxidant, anti-inflammatory, and anticancer effects. This short review discusses whether sea buckthorn may represent a “golden mean” for the treatment of cancers: It has anti-proliferation properties and can induce apoptosis and stimulate the immune system, and sea buckthorn oil counteracts many side effects of chemotherapy by restoring kidney and liver function, increasing appetite, and keeping patients in general good health. Although the anticancer activity of sea buckthorn has been confirmed by many in vitro and animal in vivo studies, the treatment and prophylactic doses for humans are unknown. Therefore, greater attention should be paid to the development of well-controlled and high-quality clinical experiments in this area.

  10. Influence of ambient and enhanced ultraviolet-B radiation on the plant growth and physiological properties in two contrasting populations of Hippophae rhamnoides

    International Nuclear Information System (INIS)

    Yang, Y.; Yao, Y.; He, H.

    2008-01-01

    Two contrasting sea buckthorn (Hippophae rhamnoides L.) populations from low and high altitude regions were employed to investigate the effects of prevailing and enhanced ultraviolet-B (UV-B) radiation on plant growth and physiological properties under a UVB-enhanced/exclusion system. The experimental design included three UV-B regimes, including excluded (-UVB), near-ambient (NA) and enhanced UV-B (+UVB) radiation. Compared with the control (-UVB), NA caused the formation of smaller but thicker plant leaves in both sea buckthorn populations, paralleled with significant increments of carotenoids and UV-absorbing compounds as well as improved water economy. NA also induced more biomass partition from shoot to root, but CO2 assimilation rate (A), photosynthetic area and biomass accumulation were unaffected. The low-altitude population seemed sensitive to +uvB, as indicated by the decreases in total biomass, A and ascorbic acid content (Asa, an antioxidant) compared with NA. However, little +UVB effect occurred on the high-altitude population, and we suggest that the higher tolerance of this population could be associated with its specific morphological and physiological characteristics, such as small but thick leaves and high-level of Asa content, as well as its greater physiological modification in response to NA, e.g., increases in protective compounds (carotenoids and UV-absorbing compounds) and improvement in water economy, in comparison to the low-altitude population, which form an effective adaptation strategy to enhanced UV-B stress

  11. Organic farming methods affect the concentration of phenolic compounds in sea buckthorn leaves

    OpenAIRE

    Heinäaho, Merja; Pusenius, Jyrki; Julkunen-Tiitto, Riitta

    2006-01-01

    - Berries, juice and other products of organically cultivated sea buckthorn (Hippophae rhamnoides L., Eleagnaceae) are in great demand because of their beneficial health effects. The availability of berries is not sufficient especially for locally growing market. However no research has been done on the effects of farming methods on the quality of sea buckthorn tissues before. We established a field experiment to study the effects of organic farming methods on the concentra-tions of phenolic ...

  12. Comparative Analysis of Total Phenolic Content in Sea Buckthorn Wine and Other Selected Fruit Wines

    OpenAIRE

    Bharti Negi; Gargi Dey

    2009-01-01

    This is the first report from India on a beverage resulting from alcoholic fermentation of the juice of sea buckthorn (Hippophae rhamnoides L) using lab isolated yeast strain. The health promoting potential of the product was evaluated based on its total phenolic content. The most important finding was that under the present fermentation condition, the total phenolic content of the wine product was 689 mg GAE/L. Investigation of influence of bottle ageing on the sea buckthorn wine showed a sl...

  13. Three new flavonoids from the seeds of Hippophae rhamnoides subsp. sinensis.

    Science.gov (United States)

    Zhang, Jing; Gao, Wen; Cao, Min-Sheng; Kong, De-Yun

    2012-01-01

    To study the chemical constituents of the seeds of Hippophae rhamnoides subsp. sinensis, three new flavonoids acylated with one monoterpenic acid, named 3-O-β-D-glucosyl-kaempferol-7-O-{2-O-[2(E)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]}-α-L-rhamnoside (3), 3-O-β-D-sophorosyl-kaempferol-7-O-{3-O-[2(E)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]}-α-L-rhamnoside (4), and 3-O-β-D-sophorosyl-kaempferol-7-O-{2-O-[2(E)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]}-α-L-rhamnoside (5), together with four known compounds, were isolated from the seeds of H. rhamnoides subsp. sinensis. Compounds 1 and 2 are reported for the first time from this genus. Their structures were elucidated on the basis of chemical and spectral analysis, including 1D and 2D NMR and HR-MS, and by comparison with literature data.

  14. On-line hyphenation of centrifugal partition chromatography and high pressure liquid chromatography for the fractionation of flavonoids from Hippophaë rhamnoides L. berries.

    Science.gov (United States)

    Michel, Thomas; Destandau, Emilie; Elfakir, Claire

    2011-09-09

    Centrifugal Partition Chromatography (CPC), a liquid-liquid preparative chromatography using two immiscible solvent systems, benefits from numerous advantages for the separation or purification of synthetic or natural products. This study presents the on-line hyphenation of CPC-Evaporative Light Scattering Detector (CPC-ELSD) with High Performance Liquid Chromatography-UV (HPLC-UV) for the fractionation of flavonols from a solvent-free microwave extract of sea buckthorn (Hippophaë rhamnoides L., Elaeagnaceae) berries. An Arizona G system was used for the fractionation of flavonoids by CPC and a fused core Halo C18 column allowed the on-line analyses of collected fractions by HPLC. The on-line CPC/HPLC procedure allowed the simultaneous fractionation step at preparative scale combined with the HPLC analyses which provide direct fingerprint of collected fractions. Thus the crude extract was simplified and immediate information on the composition of fractions could be obtained. Furthermore, this methodology reduced the time of post-fractionation steps and facilitated identification of main molecules by Mass Spectrometry (MS). Rutin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucoside, quercetin-3-O-glucoside, isorhamnetin-rhamnoside, quercetin and isorhamnetin were identified. CPC-ELSD/HPLC-UV could be considered as a high-throughput technique for the guided fractionation of bioactive natural products from complex crude extracts. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. Impacts of soil petroleum contamination on nutrient release during litter decomposition of Hippophae rhamnoides.

    Science.gov (United States)

    Zhang, Xiaoxi; Liu, Zengwen; Luc, Nhu Trung; Yu, Qi; Liu, Xiaobo; Liang, Xiao

    2016-03-01

    Petroleum exploitation causes contamination of shrub lands close to oil wells. Soil petroleum contamination affects nutrient release during the litter decomposition of shrubs, which influences nutrient recycling and the maintenance of soil fertility. Hence, this contamination may reduce the long-term growth and stability of shrub communities and consequently, the effects of phytoremediation. Fresh foliar litter of Hippophae rhamnoides, a potential phytoremediating species, was collected for this study. The litter was placed in litterbags and then buried in different petroleum-polluted soil media (the petroleum concentrations were 15, 30, and 45 g kg(-1) dry soil, which were considered as slightly, moderately and seriously polluted soil, respectively) for a decomposition test. The impacts of petroleum contamination on the release of nutrients (including N, P, K, Cu, Zn, Fe, Mn, Ca and Mg) were assessed. The results showed that (1) after one year of decomposition, the release of all nutrients was accelerated in the slightly polluted soil. In the moderately polluted soil, P release was accelerated, while Cu, Zn and Mn release was inhibited. In the seriously polluted soil, Cu and Zn release was accelerated, while the release of the other nutrients was inhibited. (2) The effect of petroleum on nutrient release from litter differed in different periods during decomposition; this was mainly due to changes in soil microorganisms and enzymes under the stress of petroleum contamination. (3) To maintain the nutrient cycling and the soil fertility of shrub lands, H. rhamnoides is only suitable for phytoremediation of soils containing less than 30 g kg(-1) of petroleum.

  16. The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate.

    Science.gov (United States)

    Kuduban, Ozan; Mazlumoglu, Muhammed Recai; Kuduban, Selma Denktas; Erhan, Ertugrul; Cetin, Nihal; Kukula, Osman; Yarali, Oguzhan; Cimen, Ferda Keskin; Cankaya, Murat

    2016-01-01

    To investigate the effect of HRE (Hippophae rhamnoides extract) on oral mucositis induced in rats with MTX. Experimental animals were divided into groups as healthy (HG), HRE+MTX (HMTX), and control group, which received MTX (MTXC). HMTX group received 50 mg/kg HRE while MTXC and HG groups received equivolume distilled water with gavage once a day. After one hour of HRE and distilled water administration, HMTX and MTXC groups received a single dose of oral MTX 5 mg/ kg. This procedure was repeated for one month. The levels of MDA, IL-1β, and TNF-α were found to be significantly higher in the cheek, lower lip, and tongue tissue of the animals receiving MTX, compared with HG and HMTX groups; however, these parameters were lower in the cheek and low lip tissue, and a milder damage ocurred in these tissues, compared with the tongue tissue in MTXC group. No histopathologic damage was observed in the cheek, lower lip, and tongue tissues of the rats treated with HRE. This findings indicate that HRE as a natural product is an important advantage compared with synthetic drugs for prophylaxis of oral mucositis developed due to MTX.

  17. The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate

    Directory of Open Access Journals (Sweden)

    Ozan Kuduban

    Full Text Available ABSTRACT Objective: To investigate the effect of HRE (Hippophae rhamnoides extract on oral mucositis induced in rats with MTX. Material and Methods: Experimental animals were divided into groups as healthy (HG, HRE+MTX (HMTX, and control group, which received MTX (MTXC. HMTX group received 50 mg/kg HRE while MTXC and HG groups received equivolume distilled water with gavage once a day. After one hour of HRE and distilled water administration, HMTX and MTXC groups received a single dose of oral MTX 5 mg/ kg. This procedure was repeated for one month. Results: The levels of MDA, IL-1β, and TNF-α were found to be significantly higher in the cheek, lower lip, and tongue tissue of the animals receiving MTX, compared with HG and HMTX groups; however, these parameters were lower in the cheek and low lip tissue, and a milder damage ocurred in these tissues, compared with the tongue tissue in MTXC group. No histopathologic damage was observed in the cheek, lower lip, and tongue tissues of the rats treated with HRE. Conclusion: This findings indicate that HRE as a natural product is an important advantage compared with synthetic drugs for prophylaxis of oral mucositis developed due to MTX.

  18. IDENTIFICATION AND FUNCTIONAL ANNOTATION OF APOPLASTIC PHOSPHOPROTEINS OF HIPPOPHAE RHAMNOIDES SEEDLINGS

    Directory of Open Access Journals (Sweden)

    Ravi Gupta

    2016-12-01

    Full Text Available Phosphorylation is a reversible switch that regulates the biological activities of the proteins. Although there are ample of reports on the plant phosphoproteome analysis, phosphorylation status of apoplastic proteins has not been investigated profoundly. Here a shotgun proteomics approach was used to identify the phosphoproteins from the apoplast of the Hippophae rhamnoides (Seabuckthorn. A total of 123 phosphoproteins were identified using an SYNAPT G2 quadrupole time-of-flight mass spectrometer (Q-ToF-MS. Functional annotation of the identified phosphoproteins using PANTHER, Gene ontology, and KEGG programs showed that the majority of proteins were associated with the transporter, nucleic acid binding and amino acid metabolic activities. Prediction of secretory nature of the identified proteins using SignalP and SecretomeP servers showed that 56 % of the proteins were secretory, while rest of the 44 % of the proteins were non-secretory. PhosPhAt 4.0 detected 534 putative phosphorylation sites in the 75 unique Arabidopsis annotated proteins, wherein 195 (36% were on the serine residue, 196 (37% were on the threonine residue and 143 (27% were detected on the tyrosine residue. Taken together, our results provide the first insight into the phosphorylation-mediated regulation of apoplastic proteins by cellular processes, which would be helpful in an in-depth understanding of the apoplastic signaling

  19. Rapid discrimination of sea buckthorn berries from different H. rhamnoides subspecies by multi-step IR spectroscopy coupled with multivariate data analysis

    Science.gov (United States)

    Liu, Yue; Zhang, Ying; Zhang, Jing; Fan, Gang; Tu, Ya; Sun, Suqin; Shen, Xudong; Li, Qingzhu; Zhang, Yi

    2018-03-01

    As an important ethnic medicine, sea buckthorn was widely used to prevent and treat various diseases due to its nutritional and medicinal properties. According to the Chinese Pharmacopoeia, sea buckthorn was originated from H. rhamnoides, which includes five subspecies distributed in China. Confusion and misidentification usually occurred due to their similar morphology, especially in dried and powdered forms. Additionally, these five subspecies have vital differences in quality and physiological efficacy. This paper focused on the quick classification and identification method of sea buckthorn berry powders from five H. rhamnoides subspecies using multi-step IR spectroscopy coupled with multivariate data analysis. The holistic chemical compositions revealed by the FT-IR spectra demonstrated that flavonoids, fatty acids and sugars were the main chemical components. Further, the differences in FT-IR spectra regarding their peaks, positions and intensities were used to identify H. rhamnoides subspecies samples. The discrimination was achieved using principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA). The results showed that the combination of multi-step IR spectroscopy and chemometric analysis offered a simple, fast and reliable method for the classification and identification of the sea buckthorn berry powders from different H. rhamnoides subspecies.

  20. Single pot synthesized gold nanoparticles using Hippophae rhamnoides leaf and berry extract showed shape-dependent differential nanobiotechnological applications.

    Science.gov (United States)

    Sharma, Bhavana; Deswal, Renu

    2018-04-04

    A facile one-pot green synthesis of gold nanoparticles (AuNPs) with different geometries was achieved using an underutilized Himalayan bioresource Hippophae rhamnoides. Aqueous leaf (LE) and berry extracts (BE) showed rapid synthesis of monodispersed spherical LEAuNPs (27 ± 3.2 nm) and anisotropic BEAuNPs (55 ± 4.5 nm) within 2 and 15 min, respectively. The Fourier-transform infrared (FTIR) spectroscopy showed involvement of polyphenolics/flavonoids in AuNPs reduction. LE AuNPs (IC 50 49 µg) exhibited higher antioxidant potential than BE AuNPs (IC 50 57 µg). Both BE nanotriangles and LE nanospheres exhibited cytotoxicity against Jurkat cell lines. These nanocatalysts also exhibited effective (80-99%) reductive degradation of structurally different carcinogenic azo dyes. Kinetic studies revealed that BE nanotriangles exhibited higher catalytic efficiency (14-67%) than LE nanospheres suggesting shape-dependent regulation of biological activities. The gas chromatography-mass spectrometry (GC-MS) analysis confirmed conversion of toxic methyl orange dye to non-toxic intermediates. Probable degradation mechanism involving adsorption and catalytic reduction of azo bonds was proposed. The present synthesis protocol provided a facile and energy saving procedure for rapid synthesis of highly stable nanoparticles with significant antioxidant and anticancer potential. This is the first report of H. rhamnoides-mediated green synthesis of multipurpose AuNPs as antioxidant, anticancer and nanocatalytic agents for treatment of dye contaminated waste water and future therapeutic applications.

  1. [Sap flux density in response to rainfall pulses for Pinus tabuliformis and Hippophae rhamnoides from mixed plantation in hilly Loess Plateau].

    Science.gov (United States)

    Lu, Sen Bao; Chen, Yun Ming; Tang, Ya Kun; Wu, Xu; Wen, Jie

    2017-11-01

    Thermal dissipation probe (TDP) was used to continuously measure the sap flux density (F d ) of Pinus tabuliformis and Hippophae rhamnoides individuals in hilly Loess Plateau, from June to October 2015, and the environmental factors, i.e., photosynthetic active radiation (PAR), water vapor pressure deficit (VPD), and soil water content (SWC), were simultaneously monitored to clarify the difference of rainfall utilization between the two tree species in a mixed plantation. Using the methods of a Threshold-delay model, stepwise multiple regression analyses, and partial correlation analyses, this paper studied the process of F d in these two species in response to the rainfall pulses and then determined the effects of environmental factors on F d . The results showed that, with the increase of rainfall, the response percentages of F d in both P. tabuliformis and H. rhamnoides increased at first but then decreased; specifically, in the range of 0-1 mm rainfall, the F d of P. tabuliformis (-16.3%) and H. rhamnoides (-6.3%) clearly decreased; in the range of 1-5 mm rainfall, the F d of P. tabuliformis decreased (-0.4%), whereas that of H. rhamnoides significantly increased (9.0%). The lower rainfall thresholds (R L ) of F d for P. tabuliformis and H. rhamnoides were 6.4 and 1.9 mm, respectively, with a corresponding time-lag (τ) of 1.96 and 1.67 days. In the pre-rainfall period, the peak time of F d of P. tabuliformis converged upon 12:00-12:30 (70%), while the F d of H. rhamnoides peaked twice, between 10:30 and 12:00 (48%) and again between 16:00 and 16:30 (30%). In the post-rainfall period, the peak time of F d of P. tabuliformis converged upon 11:00-13:00 (40%), while that of H. rhamnoides peaked twice, between 12:00 and 13:00 (52%) and again between 16:30 and 17:00 (24%). Among the environmental factors, the rank order of factors associated with the F d of both P. tabuliformis and H. rhamnoides was PAR>VPD, before rainfall. However, the rank order of factors

  2. Protective effect of total flavonoids of seabuckthorn (Hippophae rhamnoides) in simulated high-altitude polycythemia in rats.

    Science.gov (United States)

    Zhou, Ji-Yin; Zhou, Shi-Wen; Du, Xiao-Huang; Zeng, Sheng-Ya

    2012-09-28

    Seabuckthorn (Hippophae rhamnoides L.) has been used to treat high altitude diseases. The effects of five-week treatment with total flavonoids of seabuckthorn (35, 70, 140 mg/kg, ig) on cobalt chloride (5.5 mg/kg, ip)- and hypobaric chamber (simulating 5,000 m)-induced high-altitude polycythemia in rats were measured. Total flavonoids decreased red blood cell number, hemoglobin, hematocrit, mean corpuscular hemoglobin levels, span of red blood cell electrophoretic mobility, aggregation index of red blood cell, plasma viscosity, whole blood viscosity, and increased deformation index of red blood cell, erythropoietin level in serum. Total flavonoids increased pH, pO₂, Sp(O₂), pCO₂ levels in arterial blood, and increased Na⁺, HCO₃⁻, Cl⁻, but decreased K⁺ concentrations. Total flavonoids increased mean arterial pressure, left ventricular systolic pressure, end-diastolic pressure, maximal rate of rise and decrease, decreased heart rate and protected right ventricle morphology. Changes in hemodynamic, hematologic parameters, and erythropoietin content suggest that administration of total flavonoids from seabuckthorn may be useful in the prevention of high altitude polycythaemia in rats.

  3. Fabrication and Optimization of Self-Microemulsions to Improve the Oral Bioavailability of Total Flavones of Hippophaë rhamnoides L.

    Science.gov (United States)

    Guo, Ruixue; Guo, Xinbo; Hu, Xiaodan; Abbasi, Arshad Mehmood; Zhou, Lin; Li, Tong; Fu, Xiong; Liu, Rui Hai

    2017-12-01

    The purpose of this work was to improve the oral bioavailability of a poorly soluble functional food ingredient, the total flavones of Hippophaë rhamnoides L. (TFH). A self-microemulsion drug delivery system (SMEDDS) was developed to overcome the problems of poor absorption of TFH in vivo. The optimal SMEDDS significantly enhanced the solubility of TFH up to 530 times compared to that in water. The mean droplet size was 61.76 nm with uniform distribution. And the loaded system was stable at 25 °C for 3 mo with transparent appearance. The in vitro release of TFH from SMEDDS was faster and more complete than that from suspension. After oral administration of TFH-SMEDDS in rats, the relative bioavailability of TFH was dramatically improved for 3.09 times compared with the unencapsulated form. The investigation indicated the potential application of SMEDDS as a vehicle to improve the oral bioavailability of TFH. The lipid-based nanotechnology, namely self-microemulsion drug delivery system (SMEDDS) was used to improve the bioavailability and oral delivery of total flavones of Hippophaë rhamnoides L. (TFH). The relevant bioavailability of TFH could be remarkably 3-fold improved by the optimized SMEDDS. The SMEDDS produced via a simple one-step process for poorly soluble TFH to achieve a significant improvement in the bioavailability, may endorse the promising utilization of TFH in functional foods as well as pharmaceutical fields with an enhanced absorption in vivo. © 2017 Institute of Food Technologists®.

  4. Consumer-led development of novel sea buckthorn-based beverages

    DEFF Research Database (Denmark)

    Geertsen, Julie; Allesen-Holm, Bodil Helene; Giacalone, Davide

    2016-01-01

    In the context of a growing interest toward foods of local origin, this research explored consumer perceptions of novel sea-buckthorn-based beverages (SBBs), developed by combining sea-buckthorn (hippophae rhamnoides L.) berries in combination with other locally sourced ingredients. Specifically...... characteristics were the most important drivers of consumer preferences within this product category. Practical Applications This study offers new insights into consumers’ perception of novel food products of local origin. A key issue with such ingredients is that they have unique sensory properties which...... are unfamiliar to many consumers. We suggest evidence-based directions for product development and identify potential barriers to adoption that food producers should be aware of. Methodologically, this study shows a stepwise approach to consumer-driven product development that effectively differentiated between...

  5. ISSR markers for gender identification and genetic diagnosis of Hippophae rhamnoides ssp. turkestanica growing at high altitudes in Ladakh region (Jammu and Kashmir).

    Science.gov (United States)

    Das, Kamal; Ganie, Showkat Hussain; Mangla, Yash; Dar, Tanvir-Ul-Hassan; Chaudhary, Manju; Thakur, Rakesh Kumar; Tandon, Rajesh; Raina, S N; Goel, Shailendra

    2017-03-01

    Hippophae rhamnoides L. ssp. turkestanica (Elaeagnaceae) is a predominantly dioecious and wind-pollinated medicinal plant species. The mature fruits of the species possess antioxidative, anti-inflammatory, antimicrobial, anticancerous, and antistimulatory properties that are believed to improve the immune system. The identification of male and female plants in H. rhamnoides ssp. turkestanica is quite difficult until flowering which usually takes 3-4 years or more. A sex-linked marker can be helpful in establishing the orchards through identification of genders at an early stage of development. Therefore, we studied the genetic diversity of populations in Ladakh with the aim to identify a gender-specific marker using ISSR markers. Fifty-eight ISSR primers were used to characterize the genome of H. rhamnoides ssp. turkestanica, of which eight primers generated 12 sex-specific fragments specific to one or more populations. The ISSR primer (P-45) produced a fragment which faithfully segregates all the males from the female plants across all the three valleys surveyed. This male-specific locus was converted into a SCAR. Forward and reverse primers designed from this fragment amplified a 750-bp sequence in males only, thus specifying it as an informative male-specific sex-linked marker. This SCAR marker was further validated for its capability to differentiate gender on an additional collection of plants, representing three geographically isolated valleys (Nubra, Suru, and Indus) from Ladakh region of India. The results confirmed sex-linked specificity of the marker suggesting that this conserved sequence at the Y chromosome is well preserved through the populations in Ladakh region. At present, there are no reliable markers which can differentiate male from female plants across all the three valleys of Ladakh region at an early stage of plant development. It is therefore envisaged that the developed SCAR marker shall provide a reliable molecular tool for early

  6. Sea Buckthorn Oil—A Valuable Source for Cosmeceuticals

    Directory of Open Access Journals (Sweden)

    Marijana Koskovac

    2017-10-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae. is a thorny shrub that has small, yellow to dark orange, soft, juicy berries. Due to hydrophilic and lipophilic ingredients, berries have been used as food and medicine. Sea buckthorn (SB oil derived from berries is a source of valuable ingredients for cosmeceuticals. The unique combination of SB oil ingredients, in qualitative and quantitative aspects, provides multiple benefits of SB oil for internal and external use. Externally, SB oil can be applied in both healthy and damaged skin (burns or skin damage of different etiology, as it has good wound healing properties. Due to the well-balanced content of fatty acids, carotenoids, and vitamins, SB oil may be incorporated in cosmeceuticals for dry, flaky, burned, irritated, or rapidly ageing skin. There have been more than 100 ingredients identified in SB oil, some of which are rare in the plant kingdom (e.g., the ratio of palmitoleic to γ-linolenic acid. This review discusses facts related to the origin and properties of SB oil that make it suitable for cosmeceutical formulation.

  7. Evaluation of Limiting Climatic Factors and Simulation of a Climatically Suitable Habitat for Chinese Sea Buckthorn.

    Directory of Open Access Journals (Sweden)

    Guoqing Li

    Full Text Available Chinese sea buckthorn (Hippophae rhamnoides subsp. sinensis has considerable economic potential and plays an important role in reclamation and soil and water conservation. For scientific cultivation of this species across China, we identified the key climatic factors and explored climatically suitable habitat in order to maximize survival of Chinese sea buckthorn using MaxEnt and GIS tools, based on 98 occurrence records from herbarium and publications and 13 climatic factors from Bioclim, Holdridge life zone and Kria' index variables. Our simulation showed that the MaxEnt model performance was significantly better than random, with an average test AUC value of 0.93 with 10-fold cross validation. A jackknife test and the regularized gain change, which were applied to the training algorithm, showed that precipitation of the driest month (PDM, annual precipitation (AP, coldness index (CI and annual range of temperature (ART were the most influential climatic factors in limiting the distribution of Chinese sea buckthorn, which explained 70.1% of the variation. The predicted map showed that the core of climatically suitable habitat was distributed from the southwest to northwest of Gansu, Ningxia, Shaanxi and Shanxi provinces, where the most influential climate variables were PDM of 1.0-7.0 mm, AP of 344.0-1089.0 mm, CI of -47.7-0.0°C, and ART of 26.1-45.0°C. We conclude that the distribution patterns of Chinese sea buckthorn are related to the northwest winter monsoon, the southwest summer monsoon and the southeast summer monsoon systems in China.

  8. Assessment of genetic diversity using RAPD analysis in a germplasm collection of sea buckthorn

    Directory of Open Access Journals (Sweden)

    G.I. BARTISH

    2008-12-01

    Full Text Available Random amplified polymorphic DNA (RAPD markers were used to characterize a part of a sea buckthorn gene bank collected for plant breeding purposes. Molecular markers were generated in 55 cultivars and accessions, representing five subspecies of Hippophae rhamnoides L. and intraspecific hybrids between different subspecies. Sixty-three markers were used to generate a Dice's similarity coefficient matrix of pairwise comparisons between individual RAPD profiles. Cluster (UPGMA and principal co-ordinate analyses, based on this matrix, revealed clustering of plants into groups which generally correspond to their taxonomic classification or geographic origin. The analysis of molecular variance (AMOVA was found useful for estimating components of genetic variation between and within taxonomic and geographic groups of accessions and cultivars. Whereas both alternatives for grouping the material (taxonomic or geographic origin resulted in significant between-group variation, the major part of molecular variance (approximately 75% was still attributed to variation within groups. We conclude that the RAPD analysis is useful for clarification of taxonomic and geographic origin of accessions and cultivars of sea buckthorn.

  9. Phellinus hippophaëicola (Hymenochaetaceae Is Not Rare in Poland: Findings from the Pobrzeże Szczecińskie Coastland

    Directory of Open Access Journals (Sweden)

    Friedrich Stefan

    2016-12-01

    Full Text Available The occurrence of Phellinus hippophaëicola H. Jahn, a fungal pathogen of Hippophaë rhamnoides L., on the Pobrzeże Szczecińskie coastland, NW Poland, was surveyed during 2015–2016. Altogether 59 localities of Phellinus hippophaëicola were recorded, including the first Polish inland locatlities on cultivated plants of Hippophaë rhamnoides. The localities are from 18 ATPOL grid squares, including 43 localities on the Baltic Sea coast. Phellinus hippophaëicola occurred at 74% of all the localities of Hippophaë rhamnoides examined, and at ca 84% of the plant’s natural sites. These findings, and also the status of Hippophaë rhamnoides as a protected species occurring in protected habitats in Natura 2000 sites, mean that Phellinus hippophaëicola is not a rare and endangered species in Poland. In total, 69 localities located in 28 ATPOL grid squares, including the localities reported here, are currently known from Poland.

  10. A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model.

    Science.gov (United States)

    İlhan, Mert; Süntar, İpek; Demirel, Mürşide Ayşe; Yeşilada, Erdem; Keleş, Hikmet; Küpeli Akkol, Esra

    2016-12-01

    Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mixture of sea buckthorn and St. John's wort oils (HrHp oil) in the treatment of endometriosis. The activity was assessed in surgically induced endometriosis in rats. A 15-mm piece of endometrium was sutured into the abdominal wall. Twenty-eight days later, a second laparotomy was performed to calculate the endometrial foci areas and to score intra-abdominal adhesions. The rats were treated with either vehicle, HrHp oil formulation, or the reference (buserelin acetate). At the end of the experiment all rats were sacrificed and endometriotic foci areas and intra-abdominal adhesions were re-evaluated. The tissue sections were analyzed histopathologically. Peritoneal fluids of the experimental animals were collected in order to detect the levels of tumor necrosis factor-α, vascular endothelial growth factor, and interleukin-6, which might be involved in the etiology of endometriosis. In the HrHp oil-treated group, the volumes of endometriotic implants were found to be significantly decreased (from 50.8 mm 3 to 18.6 mm 3 , pnecrosis factor-α decreased from 7.02±1.33 pg/mL to 4.78±1.02 pg/mL (pvascular endothelial growth factor from 17.39±8.52 pg/mL to 9.67±5.04 pg/mL (ptreatment. HrHp oil may be a promising alternative for the treatment of endometriosis. Copyright © 2016. Published by Elsevier B.V.

  11. Effect of Hippophae leaves on neurotransmitters and hematological parameters in whole body irradiated rats

    International Nuclear Information System (INIS)

    Gupta, Vanita; Prasad, Jagdish; Madhu Bala

    2012-01-01

    Till date no approved radio-protective agent is available world over. WR-2721 had severe side effects and was behaviourally toxic even at sub-lethal doses of ionizing radiation. Seabuckthorn (Hippophae rhamnoides L.) is known for its nutraceutical and therapeutic values. Our studies demonstrated that treatment with leaves of H. rhamnoides rendered > 90% whole body radioprotection in 60 Co-g-irradiated (10 Gy) mice population in comparison to 100% death in non-Hippophae treated irradiated (10 Gy) mice population. Our studies also demonstrated that treatment with leaves of H. rhamnoides prevented conditioned taste aversion (CTA) in irradiated (2 Gy) Sprague-Dawley rats. The present study was planned to evaluate the effects of aqueous extract of Hippophae leaves on changes in levels of neurotransmitters ((acetylcholine esterase (AChE) and dopamine (DA)) in plasma and brain, haematological parameters in blood/plasma; and brain histology in Sprague-Dawley rats showing CTA after 60 Co-g-irradiation (2 Gy). The results showed that whole body 60 Co-g-irradiation (2 Gy) (i) increased the levels of Ach, Eepinephrine (E) and norepinephrine (NE); oxidative stress (MDA and NO), and (ii) decreased the levels of DA; WBC counts and RBC counts and antioxidants (GSH), in comparison to untreated control. Treatment with 12 mg/kg b.w. drug concentration, prior to irradiation significantly (p<0.05) (i) decreased the levels of AChE, E and NE, and MDA and NO levels in plasma and brain, and (ii) increased the WBC counts; RBC counts and levels of antioxidants (GSH), in comparison to radiation control group. Histological changes in brain were also recorded. The results demonstrated that Hippophae leaves extract had neuro-protective and reduced oxidative stress in brain of whole body irradiated mice and could be, thereby contributing to behavioural protection. (author)

  12. Restoration of Tear Secretion in a Murine Dry Eye Model by Oral Administration of Palmitoleic Acid

    OpenAIRE

    Nakamura, Shigeru; Kimura, Yuki; Mori, Daisuke; Imada, Toshihiro; Izuta, Yusuke; Shibuya, Michiko; Sakaguchi, Hisayo; Oonishi, Erina; Okada, Naoko; Matsumoto, Kenji; Tsubota, Kazuo

    2017-01-01

    Sea buckthorn (Hippophae rhamnoides)–derived products have traditionally been used as food and medicinal ingredients in Eastern countries. The purpose of this study was to investigate the effect of oral intake of sea buckthorn oil products on tear secretion using a murine dry eye model. Orally administered sea buckthorn pulp oil (not seed oil) restored aqueous tear secretion to its normal value under a dry eye condition. Palmitoleate (C16:1), a fatty acid present in sea buckthorn pulp oil, pr...

  13. Anti-Aging Effects of Hippophae rhamnoides Emulsion on Human Skin

    African Journals Online (AJOL)

    treated groups (R0) were not different (p < 0.01). Of note, a significant correlation between the active formulation and the improvement of the skin mechanical parameters was observed. The active formulation was found to be superior than the ...

  14. Restoration of Tear Secretion in a Murine Dry Eye Model by Oral Administration of Palmitoleic Acid.

    Science.gov (United States)

    Kimura, Yuki; Mori, Daisuke; Imada, Toshihiro; Izuta, Yusuke; Shibuya, Michiko; Sakaguchi, Hisayo; Oonishi, Erina; Okada, Naoko; Matsumoto, Kenji; Tsubota, Kazuo

    2017-04-05

    Sea buckthorn ( Hippophae rhamnoides ) -derived products have traditionally been used as food and medicinal ingredients in Eastern countries. The purpose of this study was to investigate the effect of oral intake of sea buckthorn oil products on tear secretion using a murine dry eye model. Orally administered sea buckthorn pulp oil (not seed oil) restored aqueous tear secretion to its normal value under a dry eye condition. Palmitoleate (C16:1), a fatty acid present in sea buckthorn pulp oil, preserved tear secretion and suppressed inflammatory cytokines in the lacrimal gland to the same extent as that by pulp oil. These results suggest that an oral intake of sea buckthorn pulp oil has a potency to preserve tear secretion capacity in the dry eye state and palmitoleate, its main constituent fatty acid, is an active component of the oil. This effect may enable a potent diet-based treatment for the prevention of dry eye.

  15. USE OF SEA BUCKTHORN FRUITS IN THE PASTRY MANUFACTURING

    Directory of Open Access Journals (Sweden)

    RODICA A. STURZA

    2016-04-01

    Full Text Available Different researches on optimizing the shelf life of pastries (gingerbread, sponge cakes that are produced by adding sea buckthorn flour (2 - 4 % by weight of the flour used are presented in this study. This study shows the impact of biologically active substances on structural and mechanical, physicochemical, microbiological properties as well as the antioxidant activity of products under the conditions of in vitro gastric digestion. It has been demonstrated that the sea buckthorn flour increases the porosity of pastries, reduces the wet gluten amount and this contributes to moisture loss. The organoleptic assessment indicates that the addition of 2 % sea buckthorn flour improves the appearance, the color and the consistency of pastries. Microbiological analysis showed that samples with added sea buckthorn flour exhibit microbiological stability due to the sea buckthorn chemical composition. The antiradical activity DPPH• in conditions of in vitro gastric digestion of the samples with added sea buckthorn flour increases in a positive way, indicating a clearly positive effect on health.

  16. Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

    Czech Academy of Sciences Publication Activity Database

    Puterova, Janka; Razumova, O.; Martínek, T.; Alexandrov, O.; Divashuk, M.; Kubát, Zdeněk; Hobza, Roman; Karlov, G.; Kejnovský, Eduard

    2017-01-01

    Roč. 9, č. 1 (2017), s. 197-212 ISSN 1759-6653 R&D Projects: GA ČR GBP501/12/G090 Grant - others:GA MŠk(CZ) LM2010005 Institutional support: RVO:68081707 Keywords : sex-chromosomes * repetitive sequences * silene-latifolia Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Developmental biology Impact factor: 3.979, year: 2016

  17. Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

    Czech Academy of Sciences Publication Activity Database

    Puterová, J.; Razumova, O.; Martínek, T.; Alexandrov, O.; Divashuk, M.; Kubát, Z.; Hobza, Roman; Karlov, G.; Kejnovský, E.

    2017-01-01

    Roč. 9, č. 1 (2017), s. 197-212 ISSN 1759-6653 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:61389030 Keywords : sex-chromosomes * repetitive sequences * silene-latifolia * molecular cytogenetics * arabidopsis-thaliana * genome size * evolution * organization * alignment * database * sex chromosomes * genome composition * chromosomal localization * repetitive DNA Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 3.979, year: 2016

  18. Chemical Composition of Sea Buckthorn Leaves, Branches and Bark

    Directory of Open Access Journals (Sweden)

    Gradt Ina

    2017-06-01

    Full Text Available Sea buckthorn leaves and branches presently create waste-/by-products of harvesting after pruning the plants. It is already known that sea buckthorn berries are important for their chemical composition and based on this occupy a wide field in nutrition. We raised the idea that sea buckthorn leaves, branches, and especially the bark, have also an extraordinary chemical composition like the berries. The aim of this study was to describe these by-products. For this purpose, detailed full analyses of corresponding samples from Russia (seven varieties and Germany (four varieties were performed. Especially the dry mass, fat content, proteins, carbohydrates, starch content, and crude fiber were investigated to obtain an overview. Minor components like total phenol content, metals, and water- and fat-soluble vitamins were also studied. All analytical parameters were based on an official collection of analysis methods (German ASU - amtliche Sammlung von Untersuchungsverfahren. The results of the full analysis of leaves and branches show some interesting aspects about the differences between male and female plants. Furthermore, we observed differences between Russian and German sea buckthorn varieties. Investigation of minor components showed that vitamins were present in very low amount (< 0.1 %.

  19. Pre-logging Treatment of Invasive Glossy Buckthorn (Frangula alnus Mill. Promotes Regeneration of Eastern White Pine (Pinus strobus L.

    Directory of Open Access Journals (Sweden)

    Thomas D. Lee

    2016-12-01

    Full Text Available Non-native glossy buckthorn (Frangula alnus Mill. is invasive in forests of the northeastern USA but little is known of its effects on tree regeneration. We tested whether killing buckthorn stems before logging reduces its post-logging abundance and increases the density and height of eastern white pine (Pinus strobus L. seedlings. Three 0.4 ha plots were clearcut, three were thinned, and three were left as controls. Each plot had previously been divided into three subplots that received different buckthorn treatments during the two years before logging. Buckthorn treatments were (1 stems cut at base five times; (2 stems cut once then heat killed four times; (3 untreated control. Three years post-logging, buckthorn density and stem height were unaffected by logging but equally reduced by the two buckthorn treatments. Buckthorn reduction increased density and height of pine seedlings, and seedling height also increased with logging. In the fifth year post-logging, pine height growth and biomass were greater in clearcut than in thinned treatments, greater in areas of buckthorn removal and, within treated subplots, greater in areas with low buckthorn density than in thickets of recovering buckthorn. Thus, although buckthorn inhibited regenerating pine, pre-logging destruction of buckthorn stems reduced such competition for at least four years.

  20. Exotic Invasive Shrub Glossy Buckthorn Reduces Restoration Potential for Native Forest Herbs

    Directory of Open Access Journals (Sweden)

    Caroline Hamelin

    2017-02-01

    Full Text Available Invasive glossy buckthorn could reduce restoration potential for understory native forest herbs by compromising their growth and biodiversity. Few studies of glossy buckthorn’s effects on forest herbs exist, and none were done in early-successional, partially open hardwood forests. This study was conducted in a mature hybrid poplar plantation invaded by buckthorn, located in southeastern Québec. We tested the effect of buckthorn removal on the growth of three forest herb species, whether this effect varied among species, and if canopy type (two poplar clones influenced this effect. Forest herbs were planted in herbicide (buckthorn removed and control treatments in the plantation understory, an environment similar to that of early-successional hardwood forests. Over the first two growing seasons, species showed specific reactions to buckthorn cover. Mean relative growth rate (RGR for Asarum canadense and Polygonatum pubescens was increased in the herbicide treatment (48% and 33%, respectively and decreased in the control treatment (−35% and −33%, respectively. Sanguinaria canadensis growth was the highest among species, with no difference between treatments. No effects of canopy type were detected. Results suggest that planting forest herbs for restoration purposes may be unsuccessful if buckthorn is present. Important changes in understory flora biodiversity are likely to occur over the long term in forests invaded by buckthorn.

  1. Invasive earthworms interact with abiotic conditions to influence the invasion of common buckthorn (Rhamnus cathartica).

    Science.gov (United States)

    Roth, Alexander M; Whitfeld, Timothy J S; Lodge, Alexandra G; Eisenhauer, Nico; Frelich, Lee E; Reich, Peter B

    2015-05-01

    Common buckthorn (Rhamnus cathartica L.) is one of the most abundant and ecologically harmful non-native plants in forests of the Upper Midwest United States. At the same time, European earthworms are invading previously glaciated areas in this region, with largely anecdotal evidence suggesting they compound the negative effects of buckthorn and influence the invasibility of these forests. Germination and seedling establishment are important control points for colonization by any species, and manipulation of the conditions influencing these life history stages may provide insight into why invasive species are successful in some environments and not others. Using a greenhouse microcosm experiment, we examined the effects of important biotic and abiotic factors on the germination and seedling establishment of common buckthorn. We manipulated light levels, leaf litter depth and earthworm presence to investigate the independent and interactive effects of these treatments on buckthorn establishment. We found that light and leaf litter depth were significant predictors of buckthorn germination but that the presence of earthworms was the most important factor; earthworms interacted with light and leaf litter to increase the number and biomass of buckthorn across all treatments. Path analysis suggested both direct and moisture-mediated indirect mechanisms controlled these processes. The results suggest that the action of earthworms may provide a pathway through which buckthorn invades forests of the Upper Midwest United States. Hence, researchers and managers should consider co-invasion of plants and earthworms when investigating invasibility and creating preemptive or post-invasion management plans.

  2. Out of the Qinghai-Tibet Plateau: evidence for the origin and dispersal of Eurasian temperate plants from a phylogeographic study of Hippophae rhamnoides (Elaeagnaceae)

    Czech Academy of Sciences Publication Activity Database

    Jia, Dong-Rui; Abbott, R.J.; Liu, T.-L.; Mao, K.-S.; Bartish, Igor V.; Liu, J.-Q.

    2012-01-01

    Roč. 194, č. 4 (2012), s. 1123-1133 ISSN 0028-646X Institutional research plan: CEZ:AV0Z60050516 Institutional support: RVO:67985939 Keywords : intraspecefic evolution * migration * phylogeography Subject RIV: EF - Botanics Impact factor: 6.736, year: 2012

  3. Identification of urinary biomarkers after consumption of sea buckthorn and strawberry, by untargeted LC-MS metabolomics

    DEFF Research Database (Denmark)

    Cuparencu, Catalina Sinziana; Andersen, Maj-Britt Schmidt; Gürdeniz, Gözde

    2016-01-01

    , strawberry and sea buckthorn, in humans. A randomized controlled single-blinded three-way cross-over meal study was conducted in 16 overweight men. The intervention meals consisted of sea buckthorn puree, strawberry puree or an iso-caloric control drink. Urine samples were collected on each test day at t...... in very low concentrations and therefore not identified. Nine and 11 markers were (tentatively) identified for strawberry and sea buckthorn, respectively, the most specific being conjugates of aroma compounds and coloring flavonoids. Metabolites reflecting the step-wise aromatization of quinic to hippuric...... profiling after consumption of sea buckthorn or strawberries. Combinations of these markers are proposed as potentially specific for intake of each of these berries, but need further validation in larger and less-controlled study settings....

  4. The effect of a diet supplemented with sea-buckthorn pomace on the colour and viscosity of the egg yolk

    Directory of Open Access Journals (Sweden)

    Petr Dvořák

    2017-01-01

    Full Text Available Sea-buckthorn pomace is a very valuable product which contains not only important nutrients but also high-quality oils. The question addressed in the present study was to what extent the diet containing the sea-buckthorn pomace would affect the viscosity and colour of egg yolk measured in the CIELAB system. The feeding mixture for laying hens was supplemented with 20; 50 and 100 g∙kg-1 of sea-buckthorn pomace. As a result, colour indices of the egg yolk such as L*, a* and b* changed significantly (α = 0.01. The greatest relative enhancement was observed for indicator a* for the red colour. Visually, this corresponds to the more intense orange colour of the egg yolk. The addition of sea-buckthorn pomace to the diet for laying hens resulted in a larger increase in indicator ∆E* (CIE total colour difference compared to the control group. Colour indicator hab is the only indicator whose value oscillated around that determined for the control group. The addition of sea-buckthorn pomace to the diet resulted in an increase in colour indices a*, b* and C*ab. Indicator ∆E* also increased significantly with an increasing amount of sea-buckthorn pomace in a diet. Egg yolks were darker, had more intense red and yellow colours, and showed lower viscosity which are all features preferred by the consumer.

  5. Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei.

    Science.gov (United States)

    Pop, Oana Lelia; Dulf, Francisc Vasile; Cuibus, Lucian; Castro-Giráldez, Marta; Fito, Pedro J; Vodnar, Dan Cristian; Coman, Cristina; Socaciu, Carmen; Suharoschi, Ramona

    2017-11-24

    Probiotics are bacteria that can provide health benefits to consumers and are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heat treatment and with an in vitro gastrointestinal model. The characterization of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis), high-performance liquid chromatography photodiode array detection method (HPLC-PDA), gas chromatography coupled with mass spectrometry (GS-MS) and Cryo scanning electron microscopy (Cryo-SEM). During heat treatment, the entrapped probiotic cells proved high viability (>6 CFU log/g), even at temperatures above 50 °C. The rich in monounsaturated fatty acids sea buckthorn fraction improved the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15% higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this approach may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.

  6. Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Oana Lelia Pop

    2017-11-01

    Full Text Available Probiotics are bacteria that can provide health benefits to consumers and are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heat treatment and with an in vitro gastrointestinal model. The characterization of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis, high-performance liquid chromatography photodiode array detection method (HPLC-PDA, gas chromatography coupled with mass spectrometry (GS-MS and Cryo scanning electron microscopy (Cryo-SEM. During heat treatment, the entrapped probiotic cells proved high viability (>6 CFU log/g, even at temperatures above 50 °C. The rich in monounsaturated fatty acids sea buckthorn fraction improved the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15% higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this approach may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.

  7. Sea buckthorn decreases and delays insulin response and improves glycaemic profile following a sucrose-containing berry meal

    DEFF Research Database (Denmark)

    Mortensen, Maria Wichmann; Spagner, Camilla; Cuparencu, Cătălina

    2018-01-01

    PURPOSE: Berries and mixed berry products exert acute effects on postprandial glycaemia and insulinemia, but very few berries have been studied, and primarily in normal weight subjects. Sea buckthorn and strawberry are compositionally widely different berries and may likely produce different...... responses. The effects of strawberry and sea buckthorn on postprandial glycaemia and insulinemia were examined in overweight or obese male subjects. Subjective appetite sensations and ad libitum intake were also examined. METHODS: The study was conducted as a randomised, controlled, single-blinded, three......-way crossover study. Eighteen subjects were studied in three 2-h meal tests followed by a subsequent ad libitum meal. Test meals contained added sucrose and either sea buckthorn, strawberry or no berries with added fructose (control). Blood samples were collected at t = 0, 30, 45, 60, 90 and 120 min. Subjective...

  8. Spectro colorimetric and GC-MS Models, Used to Determine the Changes in the Natural Compounds of the Sea Buck thorn Leaves Sterilized with Ionizing Radiations

    International Nuclear Information System (INIS)

    Minea, R.; Popescu, M.I.; Sima, E.; Dumitrascu, M.; Culea, M.; Manea, St.; Mazilu, E.

    2009-01-01

    Spectro colorimetric and GC-MS methods were developed for the quantitative and quality analyze of the fatty acid methyl esters (FAME) and of some natural compounds extracted from the Sea Buckthorn (Hippophae rhamnoides) leaves sterilized by treating them with accelerated electron beams, generated by a linear accelerator. The spectro colorimetric models describe and easy controls the color as it relies on the psycho physics of the color perception and on the simple colorimetric models. Hunter Lab, CIELAB, CIELCH simple colorimetric models are used in developing complex colorimetric models and for the calculation of simple colorimetric models expressed as the total color difference between a sample and a witness, ΔΕ * , ΔΕ C MC, ΔΕ * D IN99, ΔΕ * C IE2000. They provide qualitative data on the deterioration of the active compounds by irradiation. If they are validated by GC-MS methods, they can also provide quantitative data on the radioinduced changes caused to the Sea Buckthorn leaves. The developed GC-MS methods allow the validation of the spectro colorimetric methods for the quantitative and qualitative evaluation of the radioinduced changes in the Sea Buckthorn leaves, reducing both the analyze times and the analyze cost, respectively the random errors of the procedures for extraction and derivation applied to samples preparation

  9. Vitamin E and Hippophea rhamnoides L. extract reduce nicotine-induced oxidative stress in rat heart.

    Science.gov (United States)

    Gumustekin, Kenan; Taysi, Seyithan; Alp, Hamit Hakan; Aktas, Omer; Oztasan, Nuray; Akcay, Fatih; Suleyman, Halis; Akar, Sedat; Dane, Senol; Gul, Mustafa

    2010-06-01

    The effects of vitamin E and Hippophea rhamnoides L. extract (HRe-1) on nicotine-induced oxidative stress in rat heart were investigated. There were eight rats per group and supplementation period was 3 weeks. The groups were: nicotine [0.5 mg kg(-1)day(-1), intraperitoneal (i.p.)]; nicotine plus vitamin E [75 mg kg(-1)day(-1), intragastric (i.g.)]; nicotine plus HRe-1 (250 mg kg(-1)day(-1), i.g.); and the control group (receiving only vehicles). Nicotine increased the malondialdehyde level, which was prevented by both vitamin E and HRe-1. Glutathione peroxidase (GPx) activity in nicotine plus vitamin E supplemented group was higher than the others. Glutathione S-transferase (GST) activity in nicotine plus HRe-1 supplemented group was increased compared with the control group. Catalase activity was higher in nicotine group compared with others. GPx activity in nicotine plus vitamin E supplemented group was elevated compared with the others. Total and non-enzymatic superoxide scavenger activities in nicotine plus vitamin E supplemented group were lower than nicotine plus HRe-1 supplemented group. Superoxide dismutase (SOD) activity was higher in nicotine plus HRe-1 supplemented group compared with others. Glutathione reductase activity and nitric oxide level were not affected. Increased SOD and GST activities might have taken part in the prevention of nicotine-induced oxidative stress in HRe-1 supplemented group in rat heart. Flavonols such as quercetin, and isorahmnetin, tocopherols such as alpha-tocopherol and beta-tocopherol and carotenoids such as alpha-carotene and beta-carotene, reported to be present in H. rhamnoides L. extracts may be responsible for the antioxidant effects of this plant extract. 2010 John Wiley & Sons, Ltd.

  10. Comparative de novo transcriptome analysis of male and female Sea buckthorn.

    Science.gov (United States)

    Bansal, Ankush; Salaria, Mehul; Sharma, Tashil; Stobdan, Tsering; Kant, Anil

    2018-02-01

    Sea buckthorn is a dioecious medicinal plant found at high altitude. The plant has both male and female reproductive organs in separate individuals. In this article, whole transcriptome de novo assemblies of male and female flower bud samples were carried out using Illumina NextSeq 500 platform to determine the role of the genes involved in sex determination. Moreover, genes with differential expression in male and female transcriptomes were identified to understand the underlying sex determination mechanism. The current study showed 63,904 and 62,272 coding sequences (CDS) in female and male transcriptome data sets, respectively. 16,831 common CDS were screened out from both transcriptomes, out of which 625 were upregulated and 491 were found to be downregulated. To understand the potential regulatory roles of differentially expressed genes in metabolic networks and biosynthetic pathways: KEGG mapping, gene ontology, and co-expression network analysis were performed. Comparison with Flowering Interactive Database (FLOR-ID) resulted in eight differentially expressed genes viz. CHD3-type chromatin-remodeling factor PICKLE ( PKL ), phytochrome-associated serine/threonine-protein phosphatase ( FYPP ), protein TOPLESS ( TPL ), sensitive to freezing 6 ( SFR6 ), lysine-specific histone demethylase 1 homolog 1 ( LDL1 ), pre-mRNA-processing-splicing factor 8A ( PRP8A ), sucrose synthase 4 ( SUS4 ), ubiquitin carboxyl-terminal hydrolase 12 ( UBP12 ), known to be broadly involved in flowering, photoperiodism, embryo development, and cold response pathways. Male and female flower bud transcriptome data of Sea buckthorn may provide comprehensive information at genomic level for the identification of genetic regulation involved in sex determination.

  11. Studies on binding mechanism between carotenoids from sea buckthorn and thermally treated α-lactalbumin

    Science.gov (United States)

    Dumitraşcu, Loredana; Ursache, Florentina Mihaela; Stănciuc, Nicoleta; Aprodu, Iuliana

    2016-12-01

    Sea buckthorn is a natural food ingredient rich in bioactive compounds such as carotenoids, tocopherols, sterols, flavonoids, lipids, vitamins, tannins and minerals. Herein, fluorescence and UV-vis techniques were used to study the interaction of heat treated α-lactalbumin (α-LA) with carotenoids from sea buckthorn berries extract (CSB) and β-carotene. Further atomic level details on the interaction between α-LA and β-carotene were obtained by means of molecular modelling techniques. The quenching rate constants, binding constants, and number of binding sites were calculated in the presence of CSB. The emission spectral studies revealed that, CSB have the ability to bind α-LA and form a ground state complex via static quenching process. Maximum degree of quenching was reached at 100 °C, where β-carotene and CSB quenched the Trp fluorescence of α-LA by 56% and 47%, respectively. In order to reveal the interaction between CSB and α-LA, the thermodynamic parameters were determined from the van't Hoff plot based on the temperature dependence of the binding constant. In agreement with the in silico observations, the thermodynamic parameters enabled us to consider that the association between α-LA and β-carotene is a spontaneous process driven by enthalpy, dominated mainly by the van der Waals interaction, but hydrophobic interactions might also be considered. The interaction between CSB and α-LA was further confirmed by UV-vis absorption spectra, where a blue shift of position was noticed at higher temperature suggesting the complex formation. The results provided here supply a better understanding of the binding of CSB to α-LA, which can be further exploited in designing new healthy food applications.

  12. Red/Green Currant and Sea Buckthorn Berry Press Residues as Potential Sources of Antioxidants for Food Use.

    Science.gov (United States)

    Puganen, Anna; Kallio, Heikki P; Schaich, Karen M; Suomela, Jukka-Pekka; Yang, Baoru

    2018-04-04

    The potential for using extracts of press residues from black, green, red, and white currants and from sea buckthorn berries as sources of antioxidants for foods use was investigated. Press residues were extracted with ethanol in four consecutive extractions, and total Folin-Ciocalteu (F-C) reactive material and authentic phenolic compounds were determined. Radical quenching capability and mechanisms were determined from total peroxyl radical-trapping antioxidant capacity (TRAP) and oxygen radical absorbance capacity (ORAC) assays and from diphenylpicrylhydrazyl (DPPH) kinetics, respectively; specific activities were normalized to F-C reactive concentrations. Levels of total F-C reactive materials in press residue extracts were higher than in many fruits and showed significant radical quenching activity. Black currant had the highest authentic phenol content and ORAC, TRAP, and DPPH reactivity. Sea buckthorn grown in northern Finland showed extremely high total specific DPPH reactivity. These results suggest that berry press residues offer attractive value-added products that can provide antioxidants for use in stabilizing and fortifying foods.

  13. Ancient plant remains with special reference to buckthorn, Frangula alnus Mill., pyrenes from Dascyleum, Balıkesir, NW Turkey

    Directory of Open Access Journals (Sweden)

    Emel Oybak Dönmez

    2016-12-01

    Full Text Available Carbonized plant remains recovered from the ancient city Dascyleum (Daskyleion in the province of Balıkesir in northwestern Turkey provide an outline of several phases of plant use in archaic, Hellenistic, and medieval times. At the study site, various crop plant remains of Near Eastern agriculture, including cereals (barley, Hordeum vulgare L. and bread/durum/rivet wheat, Triticum aestivum L. / T. durum Desf. / T. turgidum L. and pulses [bitter vetch, Vicia ervilia (L. Willd.; grass pea, Lathyrus sativus L. / L. cicera L.; fava bean, V. faba L.; and chickpea, Cicer arietinum L.] were found. Drupaceous fruits and pyrenes of buckthorn (Frangula alnus Mill. were also found, probably representing dyes and/or medicines used by the inhabitants of the mound. Archaeometrical analyses of the ancient buckthorn pyrenes by high performance liquid chromatography with photodiode array detector (HPLC-PDA provide chemical evidence for traces of ancient mordants remaining until the present day. Some of the pulse seed remains retrieved from the medieval layers at the study site were found to have been infested by bruchid beetles (Bruchidae.

  14. Climate Change Impacts on the Potential Distribution of Eogystia hippophaecolus in China.

    Science.gov (United States)

    Li, Xue; Ge, Xuezhen; Chen, Linghong; Zhang, Linjing; Wang, Tao; Shixiang, Zong

    2018-05-28

    Seabuckthorn carpenter moth, Eogystia hippophaecolus (Hua, Chou, Fang, & Chen, 1990), is the most important boring pest of sea buckthorn (Hippophae rhamnoides L.) in the northwest of China. It is responsible for the death of large areas of H. rhamnoides forest, seriously affecting the ecological environment and economic development in northwestern China. To clarify the potential distribution of E. hippophaecolus in China, the present study used the CLIMEX 4.0.0 model to project the potential distribution of the pest using historical climate data (1981-2010) and simulated future climate data (2011-2100) for China. Under historical climate condition, E. hippophaecolus would be found to be distributed mainly between 27° N - 51° N and 74° E - 134° E, with favorable and highly favorable habitats accounting for 35.2% of the total potential distribution. Under future climate conditions, E. hippophaecolus would be distributed mainly between 27° N - 53° N and 74° E - 134° E, with the possibility of moving in a northwest direction. Under these conditions, the proportion of the total area providing a favorable and highly favorable habitat may decrease to about 33%. These results will help to identify the impact of climate change on the potential distribution of E. hippophaecolus, thereby providing a theoretical basis for monitoring and early forecasting of pest outbreaks. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  15. Influence of primitive Biłgoraj horses on the glossy buckthorn (Frangula alnus)-dominated understory in a mixed coniferous forest

    Science.gov (United States)

    Klich, Daniel

    2018-02-01

    Changes in the understory dominated by glossy buckthorn Frangula alnus via the influence of primitive horses were analyzed in a 28-year-old enclosure in the village of Szklarnia at the Biłgoraj Horse-Breeding Centre near Janów Lubelski (eastern Poland). The analysis was conducted in 20 circular plots (30 m2) defined in adjacent, similar forest stands (enclosed and control). Disturbance by the horses, mainly through trampling, caused numerous paths to form within the glossy buckthorn-dominated understory and led to a decrease in density of stems of lower height classes (30-80 and 81-130 cm, respectively). An increase in species diversity at the expense of glossy buckthorn density was also observed. The horses' trampling caused an increase in Padus avium density and the encroachment of other woody plant species that were less shade-tolerant and grew well in soils rich in nutrients. An increase in the density of woody plants over 180 cm above ground was observed within the enclosure, which was probably the result of the horses' excretion of feces. The results presented here provide new insight into the ecological role that horses play in forest-meadow landscape mosaics, which, via altering the development of vegetation, may contribute to an increase in biodiversity within forest habitats.

  16. Testing the effect of the Himalayan mountains as a physical barrier to gene flow in Hippophae tibetana Schlect. (Elaeagnaceae.

    Directory of Open Access Journals (Sweden)

    La Qiong

    Full Text Available Hippophae tibetana is a small, dioecious wind-pollinated shrub endemic to the Tibetan-Qinghai Plateau. It is one of the shrubs that occur at very high elevations (5250 m a.s.l.. The Himalayan mountains provides a significant geographical barrier to the Qinghai-Tibetan Plateau, dividing the Himalayan area into two regions with Nepal to the south and Tibet to the north. There is no information on how the Himalayan mountains influence gene flow and population differentiation of alpine plants. In this study, we analyzed eight nuclear microsatellite markers and cpDNA trnT-trnF regions to test the role of the Himalayan mountains as a barrier to gene flow between populations of H. tibetana. We also examined the fine-scale genetic structure within a population of H. tibetana on the north slope of Mount (Mt. Everest. For microsatellite analyses, a total of 241 individuals were sampled from seven populations in our study area (4 from Nepal, 3 from Tibet, including 121 individuals that were spatially mapped within a 100 m × 100 m plot. To test for seed flow, the cpDNA trnT-trnF regions of 100 individuals from 6 populations (4 from Nepal, 2 from Tibet were also sequenced. Significant genetic differentiation was detected between the two regions by both microsatellite and cpDNA data analyses. These two datasets agree about southern and northern population differentiation, indicating that the Himalayan mountains represent a barrier to H. tibetana limiting gene flow between these two areas. At a fine scale, spatial autocorrelation analysis suggests significant genetic structure within a distance of less than 45 m, which may be attributed mainly to vegetative reproduction and habitat fragmentation, as well as limited gene flow.

  17. Modulation of gamma ray induced chromosome aberrations in human peripheral blood lymphocytes by Hippophae rhammnoides leaf extract, SBL-1

    International Nuclear Information System (INIS)

    Tyagi, Anuradha; Madhu Bala

    2014-01-01

    Hippophae rhammnoides L. commonly known as seabuckthorn is a temperate shrub and native of Asia and Europe. It has high antioxidant potential and is known to the traditional Indian, Chinese and Tibetan medicinal system for treatment of multiple disorders viz., circulatory and digestive disorders, hepatic injuries, neoplasia etc. One time treatment with the standardized leaf extract from H. rhammnoides (SBL-1) before whole body irradiation with 60 Co (10 Gy), rendered more than 90% survival in non SBL-1 treated irradiated animals (J herbs, spices medi plants, 2009). Present study investigated the effects of SBL-1 treatment on chromosomal damage in human peripheral blood lymphocytes (PBL), with or without 60 Co-gamma-radiation. The lymphocytes were isolated from the blood drawn from different donors. The isolated lymphocytes were divided into several groups: Group 1-untreated control, Group 2-irradiated (2 Gy), Group 3, 4 and 5 were treated with different concentration of SBL-1, 30 min. after irradiation with 60 Co-gamma-rays (2 Gy). Group 6 was treated with the maximum concentration of SBL-1 used in the study. The metaphase spreading technique was used as per standard procedure to record chromosome breaks, dicentrics, acentrics and rings. The results were also recorded in terms of total aberrant metaphase and frequency of aberrant metaphase per 100 cells. In comparison to the untreated control, in the irradiated PBL culture, there was 8-fold increase in breaks, 211-folds in dicentrics, 75-folds in acentrics and 3-folds in rings (average data). SBL-1 alone at the highest concentration did not cause any significant change in number of breaks, dicentrics, acentrics and rings. The radiation induced aberrations decreased significantly by treatment with SBL-1 and the maximum decrease was observed when the cells were treated with 22μg/ml of SBL-1. These results demonstrated the anti-clastogenic activity of SBL-1 against gamma radiation induced damage. (author)

  18. Comparative Fingerprint of Aromatic Herbs and Yeast Alcoholic Extracts used as Ingredients for Promen, a Prostate Preventive Nutraceutical

    Directory of Open Access Journals (Sweden)

    Florina Csernatoni

    2013-11-01

    Full Text Available The aim of this study was to characterize and identify different bioactive compounds in plant sources and yeast powders  to obtain an original nutraceutical (Promen which has beneficial effects in prostate disease prevention. Seven plant and fruit sources, namely nettle (Urtica dioica, green tea (Camellia sinensis, fluff with small flowers (Epilobium parviplorum, tomato (Solanum licopersicum,  sea buckthorn (Hippophae rhamnoides, pumpkin (Cucurbita maxima, sunflower (Helianthus annus and lyophilized beer yeast (Saccharomyces cerevisiae were investigated. Methanolic extracts were prepared using 15% plant concentration and the purified fractions were analyzed using high throughput techniques like UV-VIS spectroscopy, high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD and mass spectrometry LC-QTOF -MS. The majority of the investigated plants were rich in phenolic derivatives, polyphenols (flavonoid glucosides, while yeast was rich in aminoacids, peptides and vitamins B. The major compounds identified were: Juglone, Resveratrol, Quercetin, Epigallocatechin, Gallocatechin, Biochanin A, Isorhamnetin 3-O-glucoside 7-O-rhamnoside, Quercetin 3-O-galactoside 7-O-rhamnoside, Kaempferol 3,7-O-diglucoside and p-Coumaroylquinic acid. The specific biomarkers were identified for both plant extracts used as ingredients to obtain an nutraceutical  Promen. Combined UV-Vis spectroscopy, HPLC-PDA chromatography and LC-MS spectrometry are recommended as accurate, sensible and reliable tools to investigate the plants and nutraceutical fingerprints and to predict the relation between ingredients composition and their health effects.

  19. Histopathological studies show protective efficacy of Hippophae leaf extract against damage to jejunum in whole body 60Co-a-irradiated mice

    International Nuclear Information System (INIS)

    Gupta, Manish; Prasad, Jagdish; Madhu Bala

    2012-01-01

    Background: Ionizing radiation affect living tissue by causing majority of in vivo damage by free radical production. Earlier we reported that our preparation from Hippophae leaf offered survival benefit to >90% mice population which was whole body irradiated ( 60 Co-a-rays, 10 Gy). Objective: This study was planned to examine the protective effects of our drug (from Hippophae leaf) on ( 60 Co-a-ray induced oxidative damage and histopathological changes in jejunum. Methods: Around 2 months old adult male Strain 'A' mice were irradiated (10 Gy). Drug was administered intraperitoneally (-30 mm.). Histological parameters were studied after staining the sections with hematoxylin and eosin. Malondialdehyde formation (index of lipid peroxidation), alkaline phosphatase activity, and total thiol content were determined by biochemical techniques. The data was obtained at different time interval upto 30 days. Results: Biochemical studies showed that in comparison to the untreated controls, in the irradiated (10 Gy) mice, there was significant increase in the alkaline phosphatase activity and level of malondialdehyde whereas decrease in total thiol content within 2 days. Histological studies showed that whole body irradiation (10 Gy), damaged the jejunam crypt cells and decreased the villi height within 2 days. Intra-peritoneal administration of drug, 30 mm prior to irradiation, protected the crypt cells and villi height, countered the radiation induced increase in alkaline phosphatase activity and lipid peroxidation and values were comparable to the level of control in 30 days. Conclusions: These biochemical and histopathological studies suggested that our drug can offer effective radioprotection against the oxidative damage to jejunum in vivo. (author)

  20. Restoration of Tear Secretion in a Murine Dry Eye Model by Oral Administration of Palmitoleic Acid

    Directory of Open Access Journals (Sweden)

    Shigeru Nakamura

    2017-04-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides–derived products have traditionally been used as food and medicinal ingredients in Eastern countries. The purpose of this study was to investigate the effect of oral intake of sea buckthorn oil products on tear secretion using a murine dry eye model. Orally administered sea buckthorn pulp oil (not seed oil restored aqueous tear secretion to its normal value under a dry eye condition. Palmitoleate (C16:1, a fatty acid present in sea buckthorn pulp oil, preserved tear secretion and suppressed inflammatory cytokines in the lacrimal gland to the same extent as that by pulp oil. These results suggest that an oral intake of sea buckthorn pulp oil has a potency to preserve tear secretion capacity in the dry eye state and palmitoleate, its main constituent fatty acid, is an active component of the oil. This effect may enable a potent diet-based treatment for the prevention of dry eye.

  1. The multifunctionality of berries toward blood platelets and the role of berry phenolics in cardiovascular disorders.

    Science.gov (United States)

    Olas, Beata

    2017-09-01

    Diet and nutrition have an important influence on the prophylaxis and progression of cardiovascular disease; one example is the inhibition of blood platelet functions by specific components of fruits and vegetables. Garlic, onion, ginger, dark chocolate and polyunsaturated fatty acids all reduce blood platelet aggregation. A number of fruits contain a range of cardioprotective antioxidants and vitamins, together with a large number of non-nutrient phytochemicals such as phenolic compounds, which may possess both antioxidant properties and anti-platelet activity. Fresh berries and berry extracts possess high concentrations of phenolic compounds, i.e. phenolic acid, stilbenoids, flavonoids and lignans. The aim of this review article is to provide an overview of current knowledge of the anti-platelet activity of berries, which form an integral part of the human diet. It describes the effects of phenolic compounds present in a number of berries, i.e. black chokeberries - aronia berries (Aronia melanocarpa), blueberries (Vaccinium myrtillus), cranberries (Vaccinium sect. Oxycoccus), sea buckthorn berries (Hippophae rhamnoides) and grapes (Vitis), as well as various commercial products from berries (i.e. juices), on platelets and underlying mechanisms. Studies show that the effects of berries on platelet activity are dependent on not only the concentrations of the phenolic compounds in the berries or the class of phenolic compounds, but also the types of berry and the form (fresh berry, juice or medicinal product). Different results indicate that berries may play a role in the prevention of cardiovascular disorders, but the development of well-controlled clinical studies with berries is encouraged.

  2. Berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease.

    Science.gov (United States)

    Seeram, Navindra P

    2008-02-13

    An overwhelming body of research has now firmly established that the dietary intake of berry fruits has a positive and profound impact on human health, performance, and disease. Berry fruits, which are commercially cultivated and commonly consumed in fresh and processed forms in North America, include blackberry ( Rubus spp.), black raspberry ( Rubus occidentalis), blueberry ( Vaccinium corymbosum), cranberry (i.e., the American cranberry, Vaccinium macrocarpon, distinct from the European cranberry, V. oxycoccus), red raspberry ( Rubus idaeus) and strawberry ( Fragaria x ananassa). Other berry fruits, which are lesser known but consumed in the traditional diets of North American tribal communities, include chokecherry ( Prunus virginiana), highbush cranberry ( Viburnum trilobum), serviceberry ( Amelanchier alnifolia), and silver buffaloberry ( Shepherdia argentea). In addition, berry fruits such as arctic bramble ( Rubus articus), bilberries ( Vaccinuim myrtillus; also known as bog whortleberries), black currant ( Ribes nigrum), boysenberries ( Rubus spp.), cloudberries ( Rubus chamaemorus), crowberries ( Empetrum nigrum, E. hermaphroditum), elderberries ( Sambucus spp.), gooseberry ( Ribes uva-crispa), lingonberries ( Vaccinium vitis-idaea), loganberry ( Rubus loganobaccus), marionberries ( Rubus spp.), Rowan berries ( Sorbus spp.), and sea buckthorn ( Hippophae rhamnoides), are also popularly consumed in other parts of the world. Recently, there has also been a surge in the consumption of exotic "berry-type" fruits such as the pomegranate ( Punica granatum), goji berries ( Lycium barbarum; also known as wolfberry), mangosteen ( Garcinia mangostana), the Brazilian açaí berry ( Euterpe oleraceae), and the Chilean maqui berry ( Aristotelia chilensis). Given the wide consumption of berry fruits and their potential impact on human health and disease, conferences and symposia that target the latest scientific research (and, of equal importance, the dissemination of

  3. Antimicrobial Effect of Escherichia Coli on Essential Oils Derived from Romanian Aromatic Plants

    Directory of Open Access Journals (Sweden)

    Şandru Daniela Maria

    2015-07-01

    Full Text Available This paper investigates the antimicrobial action of Escherichia coli ATCCR CRM-8739TM on the following essential oils: Teucrium marum, Pinus sylwestris, Thymus vulgaris, Salviae aethedaroleum, Cinnamomum aromaticum, Hippophae rhamnoides, Lavandula angustifolia, Abies alba, Zingiber officinale, Anethum graveolens, Coriandrum sativum, Origanum vulgare, extracted industrialy from romanian plants, using the diffusion disc method. The most intense activity was observed at the essential oil of Cinnamomum aromaticum (cinnamon and the mildest activity was observed at Zingiber officinale (ginger. Many of the essential oils tested exhibited moderate antimicrobial activity, as Teucrium marum, Thymus vulgaris, Hippophae rhamnoides, Lavandula angustifolia,Coriandrum sativum. The lowest antibacterial activity was exhibited on Pinus sylwestris, Salviae aethedaroleum, Zingiber officinale and Anethum graveolens.

  4. Identification of the traditional Tibetan medicine "Shaji" and their different extracts through tri-step infrared spectroscopy

    Science.gov (United States)

    Liu, Yue; Li, Jingyi; Fan, Gang; Sun, Suqin; Zhang, Yuxin; Zhang, Yi; Tu, Ya

    2016-11-01

    Hippophae rhamnoides subsp. sinensis Rousi, Hippophae gyantsensis (Rousi) Y. S. Lian, Hippophae neurocarpa S. W. Liu & T. N. He and Hippophae tibetana Schlechtendal are typically used under one name "Shaji", to treat cardiovascular diseases and lung disorders in Tibetan medicine (TM). A complete set of infrared (IR) macro-fingerprints of these four Hippophae species should be characterized and compared simply, accurately, and in detail for identification. In the present study, tri-step IR spectroscopy, which included Fourier transform IR (FT-IR) spectroscopy, second derivative IR (SD-IR) spectroscopy and two-dimensional correlation IR (2D-IR) spectroscopy, was employed to discriminate the four Hippophae species and their corresponding extracts using different solvents. The relevant spectra exhibited the holistic chemical compositions and variations. Flavonoids, fatty acids and sugars were found to be the main chemical components. Characteristic peak positions, intensities and shapes derived from FT-IR, SD-IR and 2D-IR spectra provided valuable information for sample discrimination. Principal component analysis (PCA) of spectral differences was performed to illustrate the objective identification. Results showed that the species and their extracts can be clearly distinguished. Thus, a quick, precise and effective tri-step IR spectroscopy combined with PCA can be applied to identify and discriminate medicinal materials and their extracts in TM research.

  5. Nutritional profile of phytococktail from trans-Himalayan plants.

    Directory of Open Access Journals (Sweden)

    Priyanka Dhar

    Full Text Available We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides, apricot (Prunus armeniaca, and roseroot (Rhodiola imbricata from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS. Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID with fatty acid methyl esters (FAMEs derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs, palmitoleic acid (43.47%, oleic acid (20.89%, and linoleic acid (4.31% were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES, and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit

  6. Protective role of Hippopahe leaves against kidney damage in total body 60Co-gamma-irradiated mice

    International Nuclear Information System (INIS)

    Saini, Manu; Prasad, Jagdish; Bala, Madhu

    2012-01-01

    Hippophae rhamnoides has diverse therapeutic applications in Indian, Chinese and Tibetan medicine. Our earlier studies have shown that pretreatment with Hippophae leaf extract rendered >90% survival in mice population. The objective of this study was to investigate the role of our herbal preparation from Hippophae leaf against radiation induced kidney damage. The study was conducted with Strain 'A' male mice weighing approximately 28 ±2 g. The mice were administered Hippophae leaf extract, 30 minutes prior to 60 cobalt-gamma irradiation. The weight of kidneys and histological changes in kidney tissues at the light microscopic level were examined at 2, 5, 7, 10 and 15 days after treatment. The results showed that no significant change was observed in kidney weight after 60 cobalt-gamma irradiation. The glomerular damage in the form of glomerular sclerosis and percentage of damaged glomeruli; tubular damage in form of tubular dilations; apoptosis, and interstitial hemorrhages in renal cortex was also observed after radiation treatment. The pretreatment with Hippophae leaf extract countered most of the histological alterations induced by radiation. In comparison to radiation alone group, there was a significant decrease (p 60 cobalt gamma radiation induced damage. (author)

  7. Seed germination of Hippophae salicifolia D

    African Journals Online (AJOL)

    Deepak Dhyani

    2012-11-08

    Nov 8, 2012 ... lead towards the development of a simple cost-effective propagation technique for large scale cultivation and ... Propagation of the species by seed is time consuming technique ..... In: Singh, V. (Eds.), Botany,. Harvesting and ...

  8. [Root anatomical structure and hydraulic traits of three typical shrubs on the sandy lands of northern Shaanxi Province, China].

    Science.gov (United States)

    Ai, Shao-shui; Li, Yang-yang; Chen, Jia-cun; Chen, Wei-yue

    2015-11-01

    Root xylem anatomical structure and hydraulic traits of three typical shrubs, i.e., Salix psammophila, Caragana korshinskii and Hippophae rhamnoides, within two soil layers (0-20 cm and 30-50 cm) were compared. The results showed that S. psammophila had a higher leaf water potential than C. korshinskii and H. rhamnoides, the average maximum and minimum lumen diameter (d(max) and d(min), respectively), the average lumen area of vessels (Alum) and the ratio of lumen area of all vessels to xylem area (Aves/Axyl) in S. psammophila roots were also significantly higher than those in C. korshinskii and H. rhamnoides, and the root vessel density (VD) in S. psammophila was the same as that in H. rhamnoides but significantly higher than that in C. korshinskii. Root hydraulic conductivity in S. psammophila was 5 times of C. korshinskii and 2.8 times of H. hamnoides. The vulnerability index in S. psammophila roots was similar to that in C. korshinskii but higher than that in H. hamnoides. S. psammophila belonged to a water-spending species, whereas both C. korshinskii and H. rhamnoides were water-saving species, and C. korshinskii was more drought-resistant than H. rhamnoides. There was no difference of d(max), d(min) and Alum between roots in two soil layers, but roots within in the 30-50 cm soil layer had larger VD and Aves/Axyl. The root specific hydraulic conductivity within the 30-50 cm soil layer was significantly higher than within the surface soil layer, whereas the vulnerability index within the 30-50 cm soil layer was smaller, indicating roots in deep soil layers had higher hydraulic transport efficiency and lower hydraulic vulnerability.

  9. Current knowledge from experimental works with radioprotective drugs from the viewpoint of latest scientific research; Aktualne poznatky z experiment8lnych prac s rastlinami s radioprotektivnym ucinkom vo svetle najnovsich vedeckych vyskumov

    Energy Technology Data Exchange (ETDEWEB)

    Skalka, J [Ustav vyzivy, dietetiky a krmovinarstva, Univerzita veterinarskeho lekarstva, 04181 Kosice (Slovakia)

    2008-06-15

    The protection of both human and animal population against a radiation impairment proves to be an impulse for continuous intensive searching for plants with radioprotective properties, identification of their radioprotective components and examination of their effects both in vivo an in vitro. I am presenting the results as well as knowledge of a latest scientific research in this field with testing the following plants: Vigna radiata, Mentha piperita, Citrus aurantium var. amara, Syzygium cumini, Tinospora cordifolia, Aegle marmelos, Phyllanthus amarus, Aloe vera, Angelica sinensis, Rosemarinus officinalis, Panax ginseng, Hippophae rhamnoides, Ocimum sanctum, Crataegus microphylla. (authors)

  10. Current knowledge from experimental works with radioprotective drugs from the viewpoint of latest scientific research

    International Nuclear Information System (INIS)

    Skalka, J.

    2008-01-01

    The protection of both human and animal population against a radiation impairment proves to be an impulse for continuous intensive searching for plants with radioprotective properties, identification of their radioprotective components and examination of their effects both in vivo an in vitro. I am presenting the results as well as knowledge of a latest scientific research in this field with testing the following plants: Vigna radiata, Mentha piperita, Citrus aurantium var. amara, Syzygium cumini, Tinospora cordifolia, Aegle marmelos, Phyllanthus amarus, Aloe vera, Angelica sinensis, Rosemarinus officinalis, Panax ginseng, Hippophae rhamnoides, Ocimum sanctum, Crataegus microphylla. (authors)

  11. Synergy between antibiotics and natural agents results in increased antimicrobial activity against Staphylococcus epidermidis.

    Science.gov (United States)

    Abidi, Syed Hani; Ahmed, Khalid; Sherwani, Sikander Khan; Kazmi, Shahana Urooj

    2015-09-27

    Staphylococcus epidermidis is one of the most frequent causes of biofilm-associated infections on indwelling medical devices. With the emergence of methicillin-resistant S. epidermidis (MRSE), there is an urgent need to discover novel active agents against a range of Gram-positive pathogens. We screened the clinical isolates of S. epidermidis for susceptibility/resistance against commonly prescribed antibiotics. Furthermore, we tested some natural agents alone and in combination with antibiotics to find possible synergistic antimicrobial effects. S. epidermidis clinical isolates were screened for susceptibility/resistance against vancomycin, erythromycin, tetracycline, chloramphenicol, ampicillin, ofloxacin, cephalexin, and gentamicin using the Kirby-Bauer disk diffusion method. The antimicrobial potential of Camellia sinensis, Juglans regia, and Hippophae rhamnoides alone and in combination with antibiotics were examined using the disk diffusion method, where the antimicrobial potential activity was measured in terms of formation of zones of inhibition. Most S. epidermidis isolates were found to be resistant to one or more antibiotics. Gentamycin and ofloxacin were found to be the most effective antibiotics against S. epidermidis isolates. Extracts of Hippophae rhamnoides, Juglans regia, and Camellia sinensis were found to be equally effective against S. epidermidis isolates. In combination with antibiotics, these extracts exhibited appreciable synergistic activity; the highest synergistic activity was observed with erythromycin and cephalexin. In the case of cephalexin, a reversion in resistance was observed. The plant extracts used in the study exhibited additive and synergistic antibacterial activity against S. epidermidis, hence providing an effective alternative to deal with the problem of multidrug resistance.

  12. Contrasting water use pattern of introduced and native plants in an alpine desert ecosystem, Northeast Qinghai–Tibet Plateau, China

    International Nuclear Information System (INIS)

    Wu, Huawu; Li, Xiao-Yan; Jiang, Zhiyun; Chen, Huiying; Zhang, Cicheng; Xiao, Xiong

    2016-01-01

    Plant water use patterns reflect the complex interactions between different functional types and environmental conditions in water-limited ecosystems. However, the mechanisms underlying the water use patterns of plants in the alpine desert of the Qinghai–Tibet Plateau remain poorly understood. This study investigated seasonal variations in the water sources of herbs (Carex moorcroftii, Astragalus adsurgens) and shrubs (Artemisia oxycephala, Hippophae rhamnoides) using stable oxygen-18 isotope methods. The results indicated that the native herbs (C. moorcroftii, A. adsurgens) and one of the shrubs (A. oxycephala) mainly relied on water from the shallow layer (0–30 cm) throughout the growing season, while the introduced shrub (H. rhamnoides) showed plasticity in switching between water from shallow and deep soil layers depending on soil water availability. All studied plants primarily depended on water from shallow soil layers early in the season. The differences of water use patterns between the introduced and native plants are closely linked with the range of active root zones when competing for water. Our findings will facilitate the mechanistic understanding of plant–soil–water relations in alpine desert ecosystems and provide information for screening introduced species for sand fixation. - Highlights: • Stable oxygen-18 in soil water experienced great evaporation enrichment. • H. rhamnoides experiences a flexible plasticity to switch between shallow and deep soil water. • Native plants mostly relied on shallow and middle soil water. • Water-use patterns by introduced-native plants are controlled by root characteristics.

  13. Radiation countermeasures from Himalayan herbs - an update

    International Nuclear Information System (INIS)

    Madhu Bala

    2012-01-01

    A successful radioprotector is the one, which essentially renders protection from the multi-organ dis-function syndrome (MODS) caused by the total body exposure to ionizing radiation. Our rationale is that and instead of a single molecule, a group of molecules/compounds working synergistically can combat the MODS more effectively. Plant extracts offer natural combinations of a plethora of compounds, which act through different mechanisms and are, therefore, the ideal choice. Plants of Himalayan regions have survived under extreme climatic conditions through millions of years and are expected to harbor a battery of anti-stress adaptive molecules offering survival benefits. Our group is actively engaged in developing composite herbal radioprotective preparations from Himalayan Plants. This group is working to develop radiation countermeasures by tapping the essential complex phytochemicals from the plants inhabiting extreme climatic zones of Himalayas. In our laboratory, systematic studies were undertaken to investigate some of these plants located at high altitude regions of Himalayas viz. Hippophae rhamnoides, Rhodiola imbricata and Podophylium hexandruin. The most effective preparation from each of these plants individually, could provide more than 80% survival benefit to the irradiated (10 Gy) mice population against zero per cent survival in non-drug treated irradiated (10 Gy) mice population. It was observed that whole extracts of plant provided much better protection than the partial extracts/fractions. It was also observed that some of the partial extracts/fractions although, provided much higher survival benefits, yet were found to be unsuitable for drug development due to much higher mutagenic and/or recombinogenic effects in comparison to the whole extracts, One of the preparations from leaves of Hippophae rhamnoides (drug) showed more than 90% survival benefits in the irradiated mice population. Only one time intra-peritoneal administration of the drug

  14. Effect of petroleum on decomposition of shrub-grass litters in soil in Northern Shaanxi of China.

    Science.gov (United States)

    Zhang, Xiaoxi; Liu, Zengwen; Yu, Qi; Luc, Nhu Trung; Bing, Yuanhao; Zhu, Bochao; Wang, Wenxuan

    2015-07-01

    The impacts of petroleum contamination on the litter decomposition of shrub-grass land would directly influence nutrient cycling, and the stability and function of ecosystem. Ten common shrub and grass species from Yujiaping oil deposits were studied. Litters from these species were placed into litterbags and buried in petroleum-contaminated soil with 3 levels of contamination (slight, moderate and serious pollution with petroleum concentrations of 15, 30 and 45 g/kg, respectively). A decomposition experiment was then conducted in the lab to investigate the impacts of petroleum contamination on litter decomposition rates. Slight pollution did not inhibit the decomposition of any litters and significantly promoted the litter decomposition of Hippophae rhamnoides, Caragana korshinskii, Amorpha fruticosa, Ziziphus jujuba var. spinosa, Periploca sepium, Medicago sativa and Bothriochloa ischaemum. Moderate pollution significantly inhibited litter decomposition of M. sativa, Coronilla varia, Artemisia vestita and Trrifolium repens and significantly promoted the litter decomposition of C. korshinskii, Z. jujuba var. spinosa and P. sepium. Serious pollution significantly inhibited the litter decomposition of H. rhamnoides, A. fruticosa, B. ischaemum and A. vestita and significantly promoted the litter decomposition of Z. jujuba var. spinosa, P. sepium and M. sativa. In addition, the impacts of petroleum contamination did not exhibit a uniform increase or decrease as petroleum concentration increased. Inhibitory effects of petroleum on litter decomposition may hinder the substance cycling and result in the degradation of plant communities in contaminated areas. Copyright © 2015. Published by Elsevier B.V.

  15. Response pattern of amino compounds in phloem and xylem of trees to soil drought depends on drought intensity and root symbiosis.

    Science.gov (United States)

    Liu, X-P; Gong, C-M; Fan, Y-Y; Eiblmeier, M; Zhao, Z; Han, G; Rennenberg, H

    2013-01-01

    This study aimed to identify drought-mediated differences in amino nitrogen (N) composition and content of xylem and phloem in trees having different symbiotic N(2)-fixing bacteria. Under controlled water availability, 1-year-old seedlings of Robinia pseudoacacia (nodules with Rhizobium), Hippophae rhamnoides (symbiosis with Frankia) and Buddleja alternifolia (no such root symbiosis) were exposed to control, medium drought and severe drought, corresponding soil water content of 70-75%, 45-50% and 30-35% of field capacity, respectively. Composition and content of amino compounds in xylem sap and phloem exudates were analysed as a measure of N nutrition. Drought strongly reduced biomass accumulation in all species, but amino N content in xylem and phloem remained unaffected only in R. pseudoacacia. In H. rhamnoides and B. alternifolia, amino N in phloem remained constant, but increased in xylem of both species in response to drought. There were differences in composition of amino compounds in xylem and phloem of the three species in response to drought. Proline concentrations in long-distance transport pathways of all three species were very low, below the limit of detection in phloem of H. rhamnoides and in phloem and xylem of B. alternifolia. Apparently, drought-mediated changes in N composition were much more connected with species-specific changes in C:N ratios. Irrespective of soil water content, the two species with root symbioses did not show similar features for the different types of symbiosis, neither in N composition nor in N content. There was no immediate correlation between symbiotic N fixation and drought-mediated changes in amino N in the transport pathways. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

  16. Solute-matrix and Solute-Solute Interactions during Supercritical Fluid Extraction of Sea Buckthorn Leaves

    Czech Academy of Sciences Publication Activity Database

    Sajfrtová, Marie; Sovová, Helena

    2012-01-01

    Roč. 42, SI (2012), s. 1682-1691 E-ISSN 1877-7058. [International Congress of Chemical and Process Engineering CHISA 2012 and 15th Conference PRES 2012 /20./. Prague, 25.08.2012-29.08.2012] R&D Projects: GA TA ČR TA01010578 Institutional support: RVO:67985858 Keywords : supercritical fluid extraction * sea buckthom leaves * solute-solute interaction Subject RIV: CI - Industrial Chemistry, Chemical Engineering

  17. Evolutionary history of an alpine shrub Hippophae tibetana (Elaeagnaceae): allopatric divergence and regional expansion

    Czech Academy of Sciences Publication Activity Database

    Jia, Dong-Rui; Liu, T.-L.; Wang, L -Y.; Zhou, D.-W.; Liu, J.-Q.

    2011-01-01

    Roč. 102, č. 1 (2011), s. 37-50 ISSN 0024-4066 Institutional research plan: CEZ:AV0Z60050516 Keywords : coalescent simulations * cpDNA * glacial refugia * orogenic processes * Quaternary glaciation Subject RIV: EF - Botanics Impact factor: 2.193, year: 2011

  18. Comparative analysis and nutritional composition of mulberry fruit morus alba plus seabuckthorn (hippophae) and their products

    International Nuclear Information System (INIS)

    Alizai, M.N.; Rehman, S.; Shah, W.H.

    2003-01-01

    The fruits of mulberry produced in Northern Pakistan were subjected to hot air dehydration to preserve without losing its natural flavour and nutrients. The dehydrated mulberry powder which is called mulberry beverage base (MBB) yielded good fruit tasty drink when mixed with suitable amount of water Shelf-life assessment was also conducted. The fresh fruit extract and the dehydrated mulberry beverage base (MBB) were analysed for juice/pulp, MBB, moisture, acidity as citric acid, total soluble solids, sugars, ascorbic acid and ash minerals like calcium, potassium, phosphorous, sodium and iron in fresh fruit extract was also determined. Mulberry pulp was mixed with seabuckthorn pulp prior to dehydration. Dehydrated product was found better in taste, colour and flavour. (author)

  19. Formulation and acceptability of foam mat dried seabuckthorn (Hippophae salicifolia) leather.

    Science.gov (United States)

    Kaushal, Manisha; Sharma, P C; Sharma, Rakesh

    2013-02-01

    Technology for utilization of seabuckthorn berries for preparation of fruit leather/bar was optimized by modifying the foam mat drying technique. The conversion of seabuckthorn juice/pulp into foam was standardized by whipping the pulp after addition of CMC @ 0-3% at 5 °C and drying the resultant foam in dehydrator (55 ± 2 °C) to a moisture content of about 12-14%. The fruit bar prepared from sulphited juice/pulp wrapped in a butter paper followed by packing in polyethylene pouches (20 g) and stored at ambient temperature (14.6-26.1 °C) experienced least changes in quality attributes like ascorbic acid (1045.7 mg/100 g vs 997.5 mg/100 g) and carotenoids (80.4 mg/100 g vs 72.3 mg/100 g) as compared to the leather made from the unsulphited pulp. Storage studies indicate that fruit bars are mildly hygroscopic (0.46-0.65) and can be stored within the RH of 46-65% at room temperature.

  20. Range expansion, genetic differentiation, and phenotypic adaption of Hippophae neurocarpa (Elaeagnaceae) on the Qinghai- Tibet Plateau

    Czech Academy of Sciences Publication Activity Database

    Kou, Y.-X.; Wu, Y.-X.; Jia, Dong-Rui; Li, Z.-H.; Wang, Y.-J.

    2014-01-01

    Roč. 52, č. 3 (2014), s. 303-312 ISSN 1674-4918 Institutional support: RVO:67985939 Keywords : genetic differentiation * phenotyp adaptation * range expansion Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 1.488, year: 2014

  1. Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection.

    Science.gov (United States)

    Wang, Wei; Lin, Ping; Ma, Lihong; Xu, Kaixuan; Lin, Xiuli

    2016-04-01

    Flavonoids are important active ingredients in many traditional Chinese medicines. In this paper, capillary electrophoresis with amperometric detection was employed to separate and detect eight flavonoids, rutin, quercetrin, quercetin, kaempferol, kaempferide, catechin, apigenin, and luteolin, in a home-made capillary electrophoresis device. Under the separation voltage of 2000 V, the eight flavonoids could be completely separated within 33 min in 18 mM borax running buffer at pH 10.2. Good linear relationships were obtained for all analytes and the detection limits for flavonoids ranged from 0.46 to 0.85 μM. Then, the method was applied to separate and determine the flavonoids in three traditional Chinese medicines, hippophae rhamnoides, hypericum perforatum, and cacumen platycladi. Finally, rutin, kaempferol, quercetin, and quercetrin were discovered in these medicines and the concentrations ranged from 0.28 to 9.94 mg/g. The recoveries of flavonoids ranged from 84.7 to 113%, which showed the high reliability of this method. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Directory of Open Access Journals (Sweden)

    Saima Jadoon

    2015-01-01

    Full Text Available The exposure to ultraviolet radiations (UVR is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS, leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  3. Quantification of the antioxidant 3, 4, 5,-Trihydroxybenzoic acid in radioprotective drug SBL-1 and its modifying effects on radiation induced changes in renal oxidative stress

    International Nuclear Information System (INIS)

    Saini, Manu; Madhu Bala

    2013-01-01

    Development of radioprotective drug is international challenge and till date no radioprotective agent has been approved for human use. Leaf extract of Hippophae rhamnoides, code name SBL-1, was demonstrated to have significant radioprotective properties. Antioxidant properties have contributed significantly to radiation protection potential of many herbs. In this study we have developed simple, sensitive, reliable, rapid and validated HPTLC protocol, for quantification of a major antioxidant 3, 4, 5,-Trihydroxybenzoic acid (Gallic acid ethyl ester) in SBL-1 and also studied the effect of treatment of mice with SBL-1 before total body irradiation (10 Gy, lethal dose) on renal anti-oxidant enzymes. Separation was carried out on silica gel 60F 254 pre-coated TLC aluminum plates, while allowing linear ascending development in twin trough glass chamber, saturated with suitably designed mobile phase. Densitometric detection of Gallic acid was at 280 nm. The protocol produced a discrete band where retention factor was 0.58; correlation coefficient for linear relationship between concentrations and peak areas was 0.9999; detection limits was 25 ng; limits of quantification was 50 ng and percentage recovery was 98.76. Administration of SBL-1 to mice before total body irradiation with lethal dose of low LET 60 Co-gamma rays (10 Gy), resulted in significant countering of the radiation induced disturbances in the levels of Glutathione S-Transferase (GST), Catalase and Superoxide dismutase (SOD) in kidney. This study elucidated an important mechanism of protection to kidney in total body lethally irradiated mice. (author)

  4. Thin-layer chromatography with stationary phase gradient as a method for separation of water-soluble vitamins.

    Science.gov (United States)

    Cimpoiu, Claudia; Hosu, Anamaria; Puscas, Anitta

    2012-02-03

    The group of hydrophilic vitamins play an important role in human health, and their lack or excess produces specific diseases. Therefore, the analysis of these compounds is indispensable for monitoring their content in pharmaceuticals and food in order to prevent some human diseases. TLC was successfully applied in the analysis of hydrophilic vitamins, but the most difficult problem in the simultaneous analysis of all these compounds is to find an optimum stationary phase-mobile phase system due to different chemical characteristics of analytes. Unfortunately structural analogues are difficult to separate in one chromatographic run, and this is the case in hydrophilic vitamins investigations. TLC gives the possibility to perform two-dimensional separations by using stationary phase gradient achieving the highest resolution by combining two systems with different selectivity. The goal of this work was to develop a method of analysis enabling separation of hydrophilic vitamins using TLC with adsorbent gradient. The developed method was used for identifying the water-soluble vitamins in alcoholic extracts of Hippophae rhamnoides and of Ribes nigrum. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. The Dynamic Trend of Soil Water Content in Artificial Forests on the Loess Plateau, China

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2016-10-01

    Full Text Available Extensive vegetation restoration projects have been widely implemented on the Loess Plateau, China, since 1998. In addition, increasing attention has been paid to the influence of revegetation on soil water. However, the response of the soil water content (SWC to vegetation construction and management has not been adequately studied. In this study, three types of typical artificial vegetation on level bench land were selected, including Pinus tabulaeformis Carr., Prunus sibirica L., and Hippophae rhamnoides Linn., with the natural grassland used as a control group in Wuqi County. The 0–160 cm SWC was monitored biweekly from August 2010 to June 2013 using a portable time domain reflectometry system. The serial autocorrelation test, Mann–Kendall trend test, and prewhitening Mann–Kendall test were employed to systematically analyze the trends in soil water dynamics. The results show that the SWC of the three selected artificial forests/shrub had a significant accumulation process in the 0–160 cm profile during the monitoring period, whereas such an increasing tendency was not observed for natural grassland. Furthermore, the greatest responses were observed in the Pinus tabulaeformis Carr. plantation.

  6. In vitro anti-Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases.

    Science.gov (United States)

    Li, Yang; Xu, Chen; Zhang, Qiang; Liu, Jun Yan; Tan, Ren Xiang

    2005-04-26

    Infection by Helicobacter pylori has been ascertained to be an important etiologic impetus leading usually to chronic active gastritis and gastric ulcer with growing incidences worldwide. Utilizing as the test pathogen a standard and five clinic strains of Helicobacter pylori, the antibacterial action was assessed in vitro with ethanol extracts of 30 Chinese herbal medicines which have been frequently prescribed since ancient times for treating gastritis-like disorders. Among the 30 tested materials, the ethanol extracts of Abrus cantoniensis (Fabaceae), Saussurea lappa (Asteraceae) and Eugenia caryophyllata (Myrtaceae) were strongly inhibitory to all test strains (MICs: approximately 40 microg/ml), and Hippophae rhamnoides (Elaeagnaceae), Fritillaria thunbergii (Liliaceae), Magnolia officinalis and Schisandra chinensis (Magnoliaceae), Corydalis yanhusuo (Papaveraceae), Citrus reticulata (Rutaceae), Bupleurum chinense and Ligusticum chuanxiong (Apiaceae) substantially active with MICs close to 60.0 microg/ml. As to antibacterial actions of the aqueous extracts of the same drugs, those derived from Cassia obtusifolia (Fabaceae), Fritillaria thunbergii and Eugenia caryophyllata were remarkably inhibitory against all the six Helicobacter pylori strains (MICs: approximately 60 microg/ml). The work compared almost quantitatively the magnitude of the anti-Helicobacter pylori actions of the 30 most prescribed gastritis-treating Chinese herbal drugs, and located as well some source plants where potent anti-Helicobacter pylori phytochemicals could be characterized.

  7. Possibilities of cultivating ornamental trees and shrubs under conditions of air pollution with oxides of sulfur

    Energy Technology Data Exchange (ETDEWEB)

    Bialobok, S.; Bartkowiak, S.; Rachwal, L.

    1974-01-01

    The field work conducted has shown that high concentrations of SO/sub 2/ in the air can be withstood by the following trees and shrubs. Trees: Acer campestris, A. platanoides, Ailanthus altissima, Aesculus hippocastanum, Morus alba, Platanus acerifolia, Pinus strobur, P. nigra, Populus Berolinensis, P. candicans, P. Hybr. 27, P. Marilandica, P. simonii, P. Serotina, Quercus robus, Robinia pseudoacacia. Shrubs: Caragana arborescens, Crataegus oxyacantha, C. monogyna, Cerasus mehaleb, Forsythia/most of the species and varieties/, Ligustrum vulgare, Philadelphus coronaria, Ptelea trifoliata, Sambucus nigra, Salix caprea, Sorbaria sorbifolia, Sorbus aucuparia, Taxus baccata. For the selection of trees and shrubs in the laboratory, high concentrations of SO/sub 2/ were used (60-150 ppm for a period of 10 minutes). Experiments were conducted on cut shrubs kept in the gas chambers. In order to estimate the degree of their injury, they were transferred to a shaded greenhouse. A concentration of 65 ppm of SO/sub 2/ could be withstood by the following Forsythias: Forsythia intermedia Primulina, F. Densiflora, F. Spectabilis, F. giraldina, F. suspensa, F. koreana, F. ovata, F. japonica and Hippophae rhamnoides. A concentration of 130 ppm could be withstood only by F. intermedia Vitelina. A similarly high concentration of SO/sub 2/ could be withstood by shoots and leaves of Ailanthus girladii Duclouxii and by Platanus acerifolia. From among the lilacs Syringa pekinensis and S. amurensis proved resistant to high concentrations of SO/sub 2/.

  8. Herbal distillates: A new era of grape marc distillates with enriched antioxidant profile.

    Science.gov (United States)

    Andreou, Vasiliki; Strati, Irini F; Fotakis, Charalambos; Liouni, Maria; Zoumpoulakis, Panagiotis; Sinanoglou, Vassilia J

    2018-07-01

    Grape marc distillates are traditional alcoholic beverages, produced mostly in the Mediterranean countries. The present study proposes the enrichment of a Greek traditional grape marc distillate (tsikoudia) with selected herbs to enhance its natural antioxidants and functional properties. Total phenolic content, the antiradical and antioxidant activities, as well as the phenolic and sugar profiles using NMR and FT-IR spectroscopy were evaluated. The enrichment of distillates with Syzygium aromaticum L., Jasminum officinale L. and Eucalyptus globulus Labill. exhibited the highest total phenolic content as well as the highest antioxidant and antiradical activities, whereas the lowest values were observed with Hippophae rhamnoides L. and Lycium Barbarum Mill. The implementation of NMR and FT-IR spectroscopies attested to the presence of phenolic compounds and of specific carbohydrates in herbal distillates, postulating their migration from selected herbal species to tsikoudia and probably contributing to their organoleptic characteristics. The target of this approach leads to new added-value distillates with enhanced characteristics. Copyright © 2018 Elsevier Ltd. All rights reserved.

  9. Soil and fertilizer amendments and edge effects on the floral succession of pulverized fuel ash

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, P. [Roehampton University, London (United Kingdom). Whitelands College

    2009-01-15

    Plots of fresh pulverized fuel ash (PFA, an industrial waste) were inoculated with soils from existing PFA sites and fertilizers in a factorial design, then left unmanaged for 12 years during which time the floral development and soil chemistry were monitored annually. For the first 3 years, the site supported a sparse mix of chenopods (including the scarce Chenopodium glaucum) and halophytes. As salinity declined, ruderals, legumes, and grasses plus the fire-site moss Funaria hygrometrica colonized, followed by Festuca arundinacea grassland (NVC community MG12) and Hippophae rhamnoides scrub. Dactylorhiza incarnata (orchidacea) appeared after 7 years, but only in plots that had received soil from existing orchid colonies. Four years later, a larger second generation of Dactylorhiza appeared, but only in the central zone of the site where vegetation was thinnest. By year 12, the site was dominated by coarse grasses and scrub, with early successional species persisting only in the sparsely vegetated center, where nitrate levels were lowest. This edge effect is interpreted as centripetal encroachment, a process of potentially wider concern for the conservation of low-fertility habitat patches. Overall, seed bank inoculation seems to have introduced few but desirable species (D. incarnata, Pyrola rotundifolia, some halophytes, and annuals), whereas initial application of organic fertilizer had long-lasting ({ge} 10 years) effects on cover and soil composition.

  10. HOST PLANTS AND CLIMATIC PREFERENCES OF THE INVASIVE SPECIES METCALFA PRUINOSA (SAY 1830 (HEMIPTERA: FLATIDAE IN SOME PLACES FROM SOUTHERN ROMANIA

    Directory of Open Access Journals (Sweden)

    Daniela Barbuceanu

    2015-12-01

    Full Text Available Observations carried out in May-September 2015 in two sites of Southern Romania reveal a rich spectrum of host plants for Metcalfa pruinosa, which consists of 204 species in 56 families. The species it is noticed on weeds and cultivated plants. The remarkable polyphagia of this species, the lack of natural enemies, and the climatic conditions of 2015 - warm and dry summer, had lead to an invasion of M. pruinosa, in the researched areas; the highest numerical abundances are noticed in shady habitats. Furthermore, on herbs, such as Levisticum officinale, Artemisia dracunculus, Ocimum basilicum, Mentha spp., usually avoided by pests, were observed colonies of the species. It is recorded high numerical abundance on fruit trees and shrubs: Hippophaë rhamnoides, Juglans regia, Prunus cerasus, Vitis vinifera, Rubus idaeus. The harmful effect occurs on apple trees Romus 1 variety as a result of the association with another pest of American origin, Eriosoma lanigerum, situation that favors the attack of the Erwinia amylovora bacteria, causing the collapse of the tree. It is found that altitudes higher than 200 m do not represent a limitative factor in the spreading of species, one of the investigated sites being located at 304 m altitude.

  11. Above-ground biomass models for Seabuckthorn (Hippophae salicifolia) in Mustang District, Nepal

    DEFF Research Database (Denmark)

    Rajchal, Rajesh; Meilby, Henrik

    2013-01-01

    weight of fruit and oven-dry weight of wood (stem and branches) and leaves were measured and used as a basis for developing biomass models. Diameters of the trees were measured at 30 cm above ground whereas the heights were measured in terms of the total tree height (m). Among several models tested......, the models suggested for local use were: ln(woody biomass, oven-dry, kg) = -3.083 + 2.436 ln(diameter, cm), ln (fruit biomass, fresh, kg) = -3.237 + 1.346 ln(diameter, cm) and ln(leaf biomass, oven-dry, kg) = -4.013 + 1.403 ln(Diameter, cm) with adjusted coefficients of determination of 0.99, 0.73 and 0.......91 for wood, fruit, and leaves, respectively. The models suggested for a slightly broader range of environmental conditions were: ln (woody biomass, oven-dry, kg) = -3.277 + 0.924 ln(diameter2 × height), ln(Fruit biomass, fresh, kg) = -3.146 + 0.485 ln(diameter2 × height) and ln(leaf biomass, oven-dry, kg...

  12. Hippophae leaf extract (SBL-1) countered radiation induced dysbiosis in jejunum of total body 60Cobalt gamma - irradiated mice

    International Nuclear Information System (INIS)

    Beniwal, C.S.; Madhu Bala

    2014-01-01

    Single dose of SBL-1 administered at the rate 30 mg/kg body weight (b.w.) 30 min prior to whole body 60 Co-gamma-irradiation at lethal dose (10 Gy), rendered >90% survival in comparison to zero survival in the non-SBL-1 treated 60 Co-gamma-irradiated (10 Gy) mice population (J Herbs Spices Med Plants, 2009; 15(2): 203-215). Present study investigated the effect of SBL-1 on jejunal microbiota in lethally irradiated mice. Study was performed with inbred Swiss albino Strain 'A' male mice (age 9 weeks) weighing 28±2 g. The animals were maintained under controlled environment at 26±2℃; 12 h light/dark cycle and offered standard animal food (Golden feed, Delhi) as well as tap water ad libitum. Metagenomic DNA was extracted, purified and quantified from jejunum of the mice. Universal primers (27f and 1492r) were used to amplify the 16S rRNA DNA from the metagenomic DNA. Amplicons were sequenced, vector contamination and chimeras were removed. The sequences (GenBank Accession No: KF681283 to KF681351) were taxonomically classified by using Sequence Match program, Ribosomal Database Project as well as by nucleotide-BLAST (E-value: 10, database: 16S rRNA gene sequences, Bacteria and Archea). Phylogenetic Tree was prepared using MEGA 5.2 package, using maximum likelihood algorithm after sequence alignment by MUSCLE. Thermus aquaticus was used as out-group to construct rooted tree. Branch stability was assessed by bootstrap analysis. Untreated animals and the animals treated with SBL-1 had 100% Lactobacillus; 60 Co gamma-irradiated animals had 55% Cohaesibacter (Alphaproteobacteria); 27% Mycoplasma (Tenericutes) and only 18% Lactobacillus; animals treated with SBL-1 prior to irradiation had 89% Lactobacillus and 11% Clostridium. This study demonstrated that treatment with SBL-1 at radioprotective doses before total body irradiation with lethal dose (10 Gy) countered the jejunal dysbiosis. (author)

  13. Protective role of plants against harmful radiation

    Energy Technology Data Exchange (ETDEWEB)

    Gautam, Shreesh Kumar; Kumar, Pawan; Singh, Abhishek; Kumar, Vikas; Bharti, Navaldey [Department of Applied Plant Science-Horticulture, Babasaheb Bhimrao Ambedkar University, Lucknow (India)

    2012-07-01

    The rapid technological advancement has increased human exposure to ionizing radiations enormously. Ionizing radiations produces deleterious effects in the living organisms. Widespread use of radiation in diagnosis therapy, industry, energy sector and inadvertent exposure during air and space travel, nuclear accidents and nuclear terror attacks requires safeguard against human exposures. Lead shielding and other physical measures can be used in such situations but with difficulty to manage; thus pharmacological intervention could be the most prudent strategy to protect humans against the harmful effect of ionizing radiations. These pharmacological agents are radioprotectives; The development of radioprotective agents has been the subject of intense research in view of their potential for use within a radiation environment. However, no ideal, safe synthetic radio protectors are available to date, so the search for alternative sources including plants has been ongoing. In Ayurveda, the traditional Indian system of medicine, several plants have been used to treat free radical-mediated ailments and, therefore, it is logical to expect that such plants may also render some protection against radiation damage. This all is due to antioxidant enzymes, nitroxides, and melatonin, antiemetic, anti-inflammatory. haemopoitic and immunostimulant compounds. Some of the plants which are found to be radioprotective are Centella asiatica, Ginkgo biloba, Hippophae rhamnoides, Ocimum sanctum, Podophyllurn hexandrum, Tinospora cordifolia, Emblica officinalis, Phyllanthus amarus, etc. So there is an urgent need to identify and characterize the many of the plants in relation to the radioprotection. Besides these medicinal plants there are also some fruits and vegetables which are having good response against harmful radiations such as Kiwifruit Actinidia deliciosa (Actinidaceae), Cape Gooseberry Physalis peruviana (Solanaceae). They protect against the radiation-induced damage by

  14. Evolving herbal formulations in management of dengue fever.

    Science.gov (United States)

    Singh, Pawan Kumar; Rawat, Pooja

    Dengue is endemic in more than 100 countries and it is estimated that annually above 390 million infections occur globally. During the period between 1996-2015, a massive increase of more than 500 per cent has been recorded in number of dengue cases reported in India. Till date, there are no specific globally accepted treatments for dengue fever in any system of medicine. Dengue does not cause very high mortality if properly handled and is currently being managed by clinicians through various adjuvant and alternative therapeutic options. Various plant based preparations have been used in different parts of India for combating dengue and are simultaneously also being scientifically validated by researchers. However, number of such scientific validation studies on phytomedicines are very less in India. Out of twenty-two plants reported against dengue, only four have been studied scientifically. Azadirachta indica, Carica papaya, Hippophae rhamnoides and Cissampelos pareira extracts were found effective and demonstrated improvement in clinical symptoms and direct inhibitory effect on dengue virus. C. papaya clinical trial showed increase in platelet count and faster recovery. These plants may be explored further as probable candidates for drug discovery against dengue. There is a need to search more such herbal formulations, which are being practiced at local level, document properly and validate them scientifically to confirm efficacy, mechanistic action and safety, before use. The herbal formulations being used by communities are the low hanging fruits which may provide alternative or adjuvant therapy if proper validation, value addition and product development steps are followed. This paper aims to review the recent status of dengue cases, deaths and evolving curative herbal solutions adapted and reported from India to combat the disease. Copyright © 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Published by Elsevier B.V. All rights

  15. [Effects of mixed decomposition of Populus simonii and other tree species leaf litters on soil properties in Loess Plateau].

    Science.gov (United States)

    Li, Qian; Liu, Zeng-Wen; Du, Liang-Zhen

    2012-03-01

    In this study, the leaf litters of Populus simonii and other 11 tree species were put into soil separately or in mixture after grinding, and incubated in laboratory to analyze the effects of their decomposition on soil properties and the interactions between the litters decomposition. The decomposition of each kind of the leaf litters in soil increased the soil urease, dehydrogenase, and phosphatase activities and the soil organic matter and available N contents markedly, but had greater differences in the effects on the soil available P content and CEC. The decomposition of the leaf litters of Caragana microphylla and of Amorpha fruticosa showed obvious effects in improving soil properties. The decomposition of the mixed leaf litters of P. simonii and Pinus tabulaeformis, Platycladus orientalis, Robinia pseudoacacia, or Ulmus pumila showed interactive promotion effects on the abundance of soil microbes, and that of the mixed leaf litters of P. simonii and P. orientalis or C. microphylla showed interactive promotion effects on the soil organic matter, available P, and available K contents and soil CEC but interactive inhibition effects on the activities of most of the soil enzymes tested. The decomposition of the mixed leaf litters of P. simonii and Larix principis-rupprechtii showed interactive promotion effects on the activities of most of the soil enzymes and soil nutrient contents, while that of the mixed leaf litters of P. simonii and P. sylvestris var. mongolica showed interactive inhibition effects. Overall, the decomposition of the mixed leaf litters of P. simo- nii and U. pumila, P. tabulaeformis, L. principis-rupprechtii, or R. pseudoacacia could improve soil quality, but the mixed leaf litters of P. simonii and P. orientalis, C. microphylla, P. sylvestris var. mongolica, Hippophae rhamnoides, or A. fruticosa showed an interactive inhibition effect during their decomposition.

  16. HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas

    Directory of Open Access Journals (Sweden)

    Raluca Maria Pop

    2013-11-01

    Full Text Available . High performance liquid chromatography (HPLC with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: Taraxacum officinalis (1, Cynara scolimus (2, Silybum marianum (3, Hypericum perforatum (4,  Chelidonium majus (5, Lycopodium clavatum (6 and  Hippophae rhamnoides (7  leaves and fruits.  Two products (A and B were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol were analyzed using HPLC-UV for their phenolics’ fingerprint and composition. The qualitative (untargeted analysis and quantitative (targeted analysis results were further compared using Principal Component Analysis (PCA in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were trans-cinnamic, chlorogenic, caffeic and p-coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids. In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.

  17. Protective role of plants against harmful radiation

    International Nuclear Information System (INIS)

    Gautam, Shreesh Kumar; Kumar, Pawan; Singh, Abhishek; Kumar, Vikas; Bharti, Navaldey

    2012-01-01

    The rapid technological advancement has increased human exposure to ionizing radiations enormously. Ionizing radiations produces deleterious effects in the living organisms. Widespread use of radiation in diagnosis therapy, industry, energy sector and inadvertent exposure during air and space travel, nuclear accidents and nuclear terror attacks requires safeguard against human exposures. Lead shielding and other physical measures can be used in such situations but with difficulty to manage; thus pharmacological intervention could be the most prudent strategy to protect humans against the harmful effect of ionizing radiations. These pharmacological agents are radioprotectives; The development of radioprotective agents has been the subject of intense research in view of their potential for use within a radiation environment. However, no ideal, safe synthetic radio protectors are available to date, so the search for alternative sources including plants has been ongoing. In Ayurveda, the traditional Indian system of medicine, several plants have been used to treat free radical-mediated ailments and, therefore, it is logical to expect that such plants may also render some protection against radiation damage. This all is due to antioxidant enzymes, nitroxides, and melatonin, antiemetic, anti-inflammatory. haemopoitic and immunostimulant compounds. Some of the plants which are found to be radioprotective are Centella asiatica, Ginkgo biloba, Hippophae rhamnoides, Ocimum sanctum, Podophyllurn hexandrum, Tinospora cordifolia, Emblica officinalis, Phyllanthus amarus, etc. So there is an urgent need to identify and characterize the many of the plants in relation to the radioprotection. Besides these medicinal plants there are also some fruits and vegetables which are having good response against harmful radiations such as Kiwifruit Actinidia deliciosa (Actinidaceae), Cape Gooseberry Physalis peruviana (Solanaceae). They protect against the radiation-induced damage by

  18. Vegetation dynamics during the Last Interglacial-Glacial cycle in the Arno coastal plain (Tuscany, western Italy): location of a new tree refuge

    Science.gov (United States)

    Lucchi, M. Ricci

    2008-12-01

    . This is because the diffusion of a characteristic pioneer vegetation (mainly represented by Hippophae cf. rhamnoides) helped to confer to the local environment a high ecological stability, buffering vegetation changes. Finally, during the Last Glacial, mixed broad-leaved deciduous and Pinus forests widely occupied the Arno coastal plain, demonstrating that this area acted as an important tree refuge. Local ecological conditions favourable to tree survival were determined mainly by (i) high precipitation, as a function of orographic uplift of air charged with moisture from the nearby Tyrrhenian Sea, (ii) mild temperatures, which were also influenced by proximity to the sea, and (iii) high topographic variability, providing a series of suitable microenvironments.

  19. Ecosystem service trade-offs and their influencing factors: A case study in the Loess Plateau of China.

    Science.gov (United States)

    Feng, Qiang; Zhao, Wenwu; Fu, Bojie; Ding, Jingyi; Wang, Shuai

    2017-12-31

    Soil erosion control (SEC), carbon sequestration (CAS), and soil moisture (SMO) strongly interact in the semi-arid Loess Plateau. Since SMO has supportive effects on SEC and CAS, it can be considered as ecosystem service (ES), and there is an immediate need to coordinate the relationships among these ecosystem services (ESs) to promote the sustainability of vegetation recovery. In this study, we quantified the ESs, ES trade-offs, and the environmental factors in 151 sample plots in the Ansai watershed, and we used a redundancy analysis (RDA) to clarify the effects of environmental factors on these ESs and their trade-offs. The results were as follows: (1) the general trend in the SEC of vegetation types was Robinia pseudoacacia (CH)>native grass (NG)>small arbor (ST)>Hippophae rhamnoides (SJ)>artificial grass (AG)>Caragana korshinskii (NT)>apple orchard (GY)>crop (CP); the CAS trend was CH>SJ>NT>AG>CP>ST>GY>NG; and the SMO trend was CP>NG>GY>AG>SJ>ST>CH>NT. (2) For SEC-SMO trade-offs, the influence of vegetation type, altitude, silt and sand composition was dominant. The arrangement of NG, AG, and SJ could decrease the extent of the trade-offs. (3) For CAS-SMO trade-offs, vegetation coverage and types were the dominant factors, but the effects were not complex. The extent of these trade-offs was lowest for NT, and that for SJ was the second lowest. (4) Considering the relationships among the three ESs, SJ was the most appropriate afforestation plant. Combing the vegetation types, slope position, slope gradient, and soil properties could regulate these ES relationships. The dominant factors influencing ES trade-offs varied among the different soil layers, so we must consider the corresponding influencing factors to regulate ESs. Moreover, manual management measures were also important for coordinating the ES relationships. Our research provides a better understanding of the mechanisms influencing the relationships among ESs. Copyright © 2017 Elsevier B.V. All

  20. A standardized seabuckthorn leaf extract (SBL-1) counters radiation induced renal histopathology, oxidative stress as well as changes in mRNA levels

    International Nuclear Information System (INIS)

    Saini, Manu; Madhu Bala; Prasad, Jagdish; Farooqi, Humaira; Abdin, M.Z.

    2014-01-01

    Hippophae rhamnoides L., (common name Seabuckthorn; Family: Elaeagnaceae) is a plant growing naturally as well as cultivated in North-West Himalayas at 7000-15,000 feet. It is known for antioxidant and medicinal properties. Our earlier studies showed that administration of SBL-1 (30 mg/kg body weight), 30 minutes before 60 Co-γ-radiation (10 Gy, lethal dose) rendered > 90% survival in mice population. The present study was planned to investigate the effects of radioprotective dose (30 mg/kg body weight) dose of SBL-1 treatment in kidneys of irradiated and control animals. Strain 'A' male mice (weighing 28±2 g) were irradiated without or 30 minutes after administration of SBL-1. Animals were sacrificed at different days (1, 2, 3, 5, 7 and 15) after the treatment. Histopathology and biochemical assays were performed using standardized procedures. Gene expression study was performed by PCR of mRNA. The 60 Co γ-irradiated animals showed a significant reduction in total thiol (T-SH) content till day 5 (p< 0.05), activity of catalase, superoxide dismutase (SOD) (p<0.01) and glutathione-s-transferase (GST) (p<0.05) and significant increase in LPx, ALP and free iron content (p<0.05) on all study days. Histopathology showed Glomeruli shrinkage, nuclear degeneration, tubular dilations, widening of tubular lumen and collapsing of cellular architecture which increased from day 2 till day 7. Significant alterations in mRNA levels of some of the key genes involved in acute renal failure were observed. In animals treated with SBL-1 before irradiation the T-SH increased significantly at day 2, 3 and 5, activity of catalase, SOD and GST decreased significantly (p<0.05) only at day 2 and 3 respectively. Significant increase in (p<0.05) LPx was observed till day 3, ALP levels only at day 3 while free iron content till day 5. Only mild changes in the tissues histology were observed at day 2, 5 and 7. By day 10 no significant difference was observed in comparison to

  1. Ethnobotany of the Balti community, Tormik valley, Karakorum range, Baltistan, Pakistan.

    Science.gov (United States)

    Abbas, Zaheer; Khan, Shujaul Mulk; Abbasi, Arshad Mehmood; Pieroni, Andrea; Ullah, Zahid; Iqbal, Muhammad; Ahmad, Zeeshan

    2016-09-09

    Limited health facilities and malnutrition are major problems in the Karakorum Range of Northern Pakistan, often resulting in various human disorders. Since centuries, however, local communities in these areas have developed traditional methods for treating various ailments and local foods capes that can be significant for devising public health and nutritional policies. This study was intended to document the ethnobotanical knowledge of the local peoples in the Tormik Valley, especially in the medical and food domains. Field trips were undertaken in 14 different villages of the study area from 2010 to 2012. Ethnobotanical data were gathered using semi-structured interviews and group conversation with 69 informants. Details about local uses of plant species were recorded along with demographic characteristics of the visited communities. Relative frequency citation index (RFCi) and preference ranking index (PRi) tools were applied to determine the cultural significance of the reported species. Sixty-three plant species, with a predominance of Asteraceae and Fabaceae family members, as well as their detailed folk uses were documented. Forty-three percent of the species were used to treat various diseases, 21 % were consumed as wild fruits and vegetables and 53 % of the species had multipurpose applications. Thymus linearis Benth, Hippophae rhamnoides ssp. turkestanica L. and Convolvulus arvensis L. were found to be the most utilized medicinal plant species, i.e. those with significant RFCi values (0.54, 0.51 and 0.48, respectively). Betula utilis D. Don was the most versatile taxon (seven different ways of utilization); being this species a common and easily accessible subalpine tree and then under anthropogenic pressure, the implementation of concrete strategies aimed at its in-situ and ex-situ conservation is strongly recommended. The valleys in the Karakorum Mountains in the Northern Pakistan host significant Traditional Knowledge on local food and medicinal

  2. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    purple willow (Salix purpurea). Only the 25% of the interventions was accomplished by the use of secondary plant species, as tamarisk (Tamarix spp.,) blackthorn (Prunus spinosa) , whitethorn (Crataegus spp.), sea-buckthorn (Hipphopae rhamnoides), wild pear (Pyrus pyraster), cottonwood (Populus nigra), eglantine (Rosa spp.), goat-willow (Salix caprea) and cornel (Cornus sanguinea). Better results were achieved with Spanish Broom, a very rural plant that can effectively colonise even poor soils like badlands; as a matter of fact, more than the 75% of the interventions had positive outcomes The efficacy of the consolidation work by the presence of living structures point out an increase of the stability of those interventions older than 4 years, with taking root species present from 54% to 78%. So far, the construction and the reliability of the works have been monitored, in order to capture critical aspects for the success of works and to build a geo-referenced data base of the existing works and their status.

  3. Environ: E00128 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available C10906], D-Glucose [CPD:C00031], Sucrose [CPD:C00089], (Zizyphusarabinan | Zizyphus saponin...68] Same as: D06758 Rhamnaceae (buckthorn family) Jujube fruit Major component: Zizyphus saponin ...

  4. Final Environmental Assessment for the Deactivation/Facility Disposition of Atlas Space Launch Complex (SLC-36) at Cape Canaveral Air Force Station, Florida

    Science.gov (United States)

    2005-08-01

    canopy, which is constantly pruned and shaped by windborne salt spray. Coastal strand forms a dense thicket of shrubs, usually dominated by live...oak (Quercus virginiana ), buckthorn (Bumelia [Sideroxylon] tenax), sea grape (Coccoloba uvifera), wax myrtle, and saw palmetto (Serenoa repens...Station #4. Coastal oak scrub consists of dense, salt- pruned thickets of live oak, sand live oak, myrtle oak, and buckthorn, sometimes densely

  5. Variable Isotopic Compositions of Host Plant Populations Preclude Assessment of Aphid Overwintering Sites

    Directory of Open Access Journals (Sweden)

    Michael S. Crossley

    2017-12-01

    Full Text Available Soybean aphid (Aphis glycines Matsumura is a pest of soybean in the northern Midwest whose migratory patterns have been difficult to quantify. Improved knowledge of soybean aphid overwintering sites could facilitate the development of control efforts with exponential impacts on aphid densities on a regional scale. In this preliminary study, we explored the utility of variation in stable isotopes of carbon and nitrogen to distinguish soybean aphid overwintering origins. We compared variation in bulk 13C and 15N content in buckthorn (Rhamnus cathartica L. and soybean aphids in Wisconsin, among known overwintering locations in the northern Midwest. Specifically, we looked for associations between buckthorn and environmental variables that could aid in identifying overwintering habitats. We detected significant evidence of correlation between the bulk 13C and 15N signals of soybean aphids and buckthorn, despite high variability in stable isotope composition within and among buckthorn plants. Further, the 15N signal in buckthorn varied predictably with soil composition. However, lack of sufficient differentiation of geographic areas along axes of isotopic and environmental variation appears to preclude the use of carbon and nitrogen isotopic signals as effective predictors of likely aphid overwintering sites. These preliminary data suggest the need for future work that can further account for variability in 13C and 15N within/among buckthorn plants, and that explores the utility of other stable isotopes in assessing likely aphid overwintering sites.

  6. Effect of fluorescent light on quality and stability of edible fats and oils

    International Nuclear Information System (INIS)

    Ahmed, T.; Atta, S.; Sohail, M.; Khan, A.R.; Akhter, S.

    2011-01-01

    Photo oxidative stress were applied to commonly consumed edible oil and fat i.e., animal fat (AF), vanaspati ghee (VG), sunflower oil (SFO), desi ghee (DG), rapeseed oil (RSO), soybean oil (SBO) as well as sea buckthorn seed oil (SB Seed oil) and sea buckthorn pulp oil (SB Pulp oil). The changes in their quality parameters i.e. free fatty acid (FFA), peroxide value (POV), beta-carotene and color (OD) were determined. Photo oxidative stress significantly (p < 0.05) increased the FFA, POV and OD, however, concentration of beta carotene decreased in all the samples with the increase in storage time. The Sea buckthorn oil was found to be more stable than other oils owing to the presence of tocopherol contents and beta-carotene. (author)

  7. In silico–based combinatorial pharmacophore modelling and ...

    Indian Academy of Sciences (India)

    Docking with Discovery Studio 3.5 revealed that two compounds from H. salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 receptor and play a role in its inhibition; whereas in the ...

  8. Nogmaals de Bijenorchis (Ophrys apifera Hudson) in het Delta-gebied

    NARCIS (Netherlands)

    Sipkes, C.

    1988-01-01

    Some 200 specimens of Ophrys apifera were found in a moist, formerly saline but now desalinated terrain along one of the new dykes connecting the former islands in the province of Zeeland. The lasting occurrence of the orchid is threatened by mice and by Salix and Hippophaë scrub overgrowing the

  9. Final Environmental Assessment of Installation Development at Grand Forks Air Force Base, North Dakota

    Science.gov (United States)

    2010-07-01

    buckthorn (a highly invasive exotic species), chokecherry (Prunus virginiana), and wood rose (Rosa woodsii) are common understory species. Wood nettle ...Laportea canadensis), stinging nettle (Urtica dioica), beggars-ticks (Bidens frondosa), and waterleaf (Hydrophyllum viginianum) are typical forbs...fertility, good water-holding capacity, and deep or thick effective rooting zones, and that are not subject to periodic flooding. Under the Farmland

  10. Supercritical Fluid Extraction of Minor Components of Vegetable Oils: beta-Sitosterol

    Czech Academy of Sciences Publication Activity Database

    Sovová, Helena; Galushko, A.A.; Stateva, R.P.; Rochová, Kristina; Sajfrtová, Marie; Bártlová, Milena

    2010-01-01

    Roč. 101, č. 2 (2010), s. 201-209 ISSN 0260-8774 R&D Projects: GA MŠk 2B06024 Institutional research plan: CEZ:AV0Z40720504 Keywords : supercritical fluid extraction * sea buckthorn oil * beta-sitosterol Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 2.168, year: 2010

  11. Effects of β-carotene on the thermal oxidation of fatty acids

    African Journals Online (AJOL)

    Alamzeb

    It is used in industries as food colorant and a source of vitamin A. The thermal induced ... sea buckthorn, palm and soybean oils (Puspitasari-. Nienaber et al. ... All-E-β-carotene (97.0%) was purchased from Sigma Aldrich USA, ... a local market in Graz, Austria. ... linoleic acid (53.9 %) in sunflower oil as compared to the.

  12.  Invasibility of three major non-native invasive shrubs and associated factors in Upper Midwest U.S. forest lands

    Science.gov (United States)

    W. Keith Moser; Zhaofei Fan; Mark H. Hansen; Michael K. Crosby; Shirley X. Fan

    2016-01-01

    We used non-native invasive plant data from the US Forest Service’s Forest Inventory and Analysis (FIA) program, spatial statistical methods, and the space (cover class)-for-time approach to quantify the invasion potential and success ("invasibility") of three major invasive shrubs (multiflora rose, non-native bush honeysuckles, and common buckthorn...

  13. Environmental Assessment - Construct a Ground-to-Air Transmitter and Receiver (GATR) Facility at Grand Forks Air Force Base

    Science.gov (United States)

    2006-06-06

    sponges, flatworms, nematode worms, segmented worms, snails, clams, and immature and adult insects , fish, amphibians, turtles, and aquatic birds and...buckthorn (a highly invasive exotic species), chokecherry, and wood rose (Rosa woodsii) are common in the under story in this area. Wood nettle ...Laportea canadensis), stinging nettle (Urtica dioica), beggars’ ticks (Bidens frondosa), and waterleaf (Hydrophyllum viginianum) are typical forbes. A

  14. Area Strip Mine Reclamation Using Dredged Material: A Field Demonstration.

    Science.gov (United States)

    1980-07-01

    COMMON BEGGAR’S TICK Bidens frondosa COMMON EVENING PRIMROSE Oenothera biennis COMMON ORACH Atriplex patula COMMON REED Phraginites communis...altissima ACERACEAE BOX ELDER Acer negundo RHAMNACEAE COMMON BUCKTHORN Rhamnus cathartica A8 r ONAGRACEAE COMMON EVENING PRIMROSE Oenothera biennis

  15. The effect of partial substitution of pork back fat with vegetable oils and walnuts on chemical composition, texture profile and sensorial properties of meatloaf

    Directory of Open Access Journals (Sweden)

    Gabriel Dănuţ MOCANU

    2015-08-01

    Full Text Available The present study investigates the effects of the partial substitution of the pork back fat with different vegetable oils (sea buckthorn, walnut and sunflower and walnuts on the chemical composition, texture profile and sensory characteristics of meatloaves. The dry matter and ash content of meatloaf with vegetable oils and walnuts were higher than the control sample (P < 0.05. The cooking loss, energy values and lipid oxidation for the samples with walnuts and vegetable oils were lower than the control sample. The meatloaf sample containing walnuts and sea buckthorn oil had the highest total antioxidant capacity. The partial substitution of pork back fat showed a positive effect on textural and sensorial characteristics. Results reveal that the incorporation of vegetable oils and walnuts has successfully reduced the animal fat content in the finite products while improving the quality characteristics.

  16. Drug: D06758 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available phus jujuba [TAX:326968] ... Same as: E00128 Therapeutic category: 5100 ... Rhamnaceae (buckthorn family) Jujube fruit Major component: Zizyphus saponin ... PubChem: 47208409 ... ...ctose [CPD:C00095 C10906], D-Glucose [CPD:C00031], Sucrose [CPD:C00089], (Zizyphusarabinan | Zizyphus sapo...nin I-III), Maslinic acid, Alphitolic acid [CPD:C16912], Jujuboside A [CPD:C17831],

  17. Urban trees and forests of the Chicago region

    Science.gov (United States)

    David J. Nowak; Robert E. III Hoehn; Allison R. Bodine; Daniel E. Crane; John F. Dwyer; Veta Bonnewell; Gary Watson

    2013-01-01

    An analysis of trees in the Chicago region of Illinois reveals that this area has about 157,142,000 trees with tree and shrub canopy that covers 21.0 percent of the region. The most common tree species are European buckthorn, green ash, boxelder, black cherry, and American elm. Trees in the Chicago region currently store about 16.9 million tons of carbon (61.9 million...

  18. Draft Environmental Impact Statement. Space Shuttle Advanced Solid Rocket Motor Program

    Science.gov (United States)

    1988-12-01

    species, including orchids ( Orchidaceae ) and several carnivorous plants, such as sundews (Drosera sp.), pitcher plants (Sarracenia sp.) and pipeworts...a dense shrub cover including saw palmetto, sea grape (Coccoloba urifera), wax myrtle (Myrica cerifera), and tough buckthorn (Bumelia tenax) (George...Cherry Prunus serotina Shrubs Galberries Ilex glabra, I. coriacea Wax Myrtle yrica cerifeFa Vines Bamboo Vine Similax laurifolia Poison Ivy Rhus radicans

  19. Intoxication with natural sources

    International Nuclear Information System (INIS)

    Toropilova, D.; Toropila, M.; Tomko, M.; Takac, L.; Fric, M.

    2015-01-01

    The authors of this article deal with intoxication of the organism with natural resources. As objects of our interest we chose some interchangeable types of fungi which cause severe intoxication of gastrointestinal tract: Scleroderma citrinum, Boletus luridus var. rubriceps (Maire) Dermek, Boletus satanas, Agaricus xanthodermus and poisonous plants affect mainly digestive tract such as Sambucus nigra L. (elderberry). Sambucus ebulus L. (European dwarf elder), Viburnum lantana L. (wayfaring tree), Viburnum opulus (guelder-rose), Rhamnus cathartica (purging buckthorn).

  20. Stanovení flavonoidních látek ve vybraných druzích léčivých rostlin používaných v potravinářství

    OpenAIRE

    Bačová, Romana

    2015-01-01

    In plant and food research the functional significance of herbs, spices and other plants, including their components is very popular topic. Plant material contains many components that are beneficial to human health by reducing the risk of chronic degenerative diseases. It is necessary to define the individual substances to understand and explain their biological effects. The first part of thesis describes the selected plants (elderberry, nettle, marigold, milk thistle, sea buckthorn, sage, p...

  1. [Nutrition value of wild-growing fruits from mountain Dagestan and its safety after fast freezing and cold storage].

    Science.gov (United States)

    Guseynova, B M

    2016-01-01

    Results of research of mineral composition, content of vitamin C and P, titrable acids, pectinaceous and phenol substances in fresh, fast-frozen (t=-30 °C), and also stored within 3 and 9 months (t=-18 °C) fruits of wild-growing blackberry, cornel, medlar and sea-buckthorn are presented in article. Determination of mineral composition was carried out by flame atomic absorption photometry, vitamin C and pectinaceous substances -by titirimetric methods, phenolic substances and vitamin P - by colorimetric methods. Vitamin C content was minimal in fresh fruits of cornel (6.9±0.3 mg%), amounted to 21.7-32.0 mg% in the fruits of blackberries and medlar and reached 180.1±7.2 mg% in the fruit of sea-buckthorn. Vitamin P concentration ranged from 34.9 (sea-buckthorn) to 180.0 mg% (cornel). Berries of a cornel contained also the greatest number of titrable acids (33.2±1.3 g/dm3), phenolic compounds (243.0±4.8 mg%) and pectinaceous substances (2.91±0.08%). The most significant content of potassium (521±15.6 mg%), calcium (133.2±5.2 mg%), magnesium (62.4±2.5 mg%) and iron (2.81±0.05 mg%) was revealed in medlar fruits. Consumption of 100 g of the studied fruits provides daily requirements of a human body, depending on a species of wild plants: in calcium -for 2-13.3%, potassium - for 7.0-20.8%, magnesium - for 8.1-15.6%, iron - for 5.9-19.2% and in vitamin C - from 5.8-24.6 to 145.7% in the case of sea buckthorn. The applied processing method of conservation - fast freezing (t=-30 °С) of fruits and their long storage (t=-18 °С) is the effective way ensuring high safety of nutrients in them. In the studied berries after 9-months cold storage the safety of vitamin C varied ranging from 55.7 (blackberry) to 76.1% (cornel), and vitamin P - from 81.9 (sea-buckthorn) to 92.8% (cornel). Stability of titrable acids, except for medlar fruits, varied from 84.2% (blackberry) to 94.0% (sea-buckthorn). The safety of phenolic and pectinaceous compounds by the end of 9 months

  2. USE OF DIETARY CAMELINA (CAMELINA SATIVA SEEDS DURING THE FINISHING PERIOD; EFFECTS ON BROILER PERFORMANCE AND ON THE ORGANOLEPTIC TRAITS OF BROILER MEAT

    Directory of Open Access Journals (Sweden)

    GEORGETA CIURESCU

    2007-05-01

    Full Text Available The study quantified the effects of Camelina seeds and buckthorn meal on broiler carcass production and quality. Camelina seeds and buckthorn meal were incorporated in the compound feeds for finishing broilers as natural source of vitamins (beta- carotene, vitamins C, B1, B2, E, F, calcium, phosphorus, magnesium, potassium, sodium, and iron. The experiment was conducted on 600 Hybro PN broilers during the age period 7 – 42 days. The broilers were assigned to 3 groups, a control group © and two experimental groups (E1 and E2, each with 200 broilers (3 groups  3 replicates  100 broilers. Three (phase-feeding compound feeds formulations were used. The control diet consisted mainly of corn, full fat soybean, soybean meal and corn gluten. Camelina seeds (10% replaced full fat soybean in the experimental groups, while additionally, in E2 the classical premix with synthetic vitamins and minerals was replaced by buckthorn meal. The partial results show that the use of Camelina seeds reduced significantly (P<0.05 the final live weight, but the liveability percentage was not influenced by the type of dietary compound feed throughout the experimental period. Carcass fat decreased by 61.44% and 30.72% in the experimental groups compared to the control group. Total proteins increased in average by 3-10% concomitantly with the increase of the water content, fat decreased in average by 1.5-4%, the energy value also decreased proportionally with the fat in the experimental groups compared to the control group. Water retention capacity increased by 1% in E2.

  3. Investigation of radiation gamma-sterilization effect on sodium alginate hydrogels

    International Nuclear Information System (INIS)

    Valueva, M.I.; Oltarzhevskaya, N.D.; Maksimova, Yu.S.; Fenin, A.A.

    2012-01-01

    The effect of gamma-radiation in doses ensuring materials sterility (6-15 kGy) on alginate hydrogels is studied. For conservation of polymer solutions viscosity after sterilization it is necessary to introduce in polymer matrix different additives inhibiting the radiolysis process. Substances-antioxidants (aqueous extracts of sea-buckthorn and blueberry), polyvinylpyrrolidone, pectin, polyethylene oxides with molecular mass from 400 to 40000 have been studied as the additives. The additives selected have the positive effect on the result of alginate hydrogels sterilization. It is established, that the problem of human tissue radiation protection is closely connected with the problem of biopolymer hydrogels radiation protection [ru

  4. Preliminary Studies Regarding the Production of Jam from Organic Rose Petal

    Directory of Open Access Journals (Sweden)

    Ana Cornelia BUTCARU

    2017-11-01

    The final products, seventeen variants of rose jam, were analyzed and tasted. Sensorial analysis was made by consumers of different ages and gender. Jam appearance, general taste, aroma, and the overall impression were noticed. V4 variant - Brother Cadfael with sea buckthorn was the most appreciated variant. For each of these top variants, target group by gender and age was analyzed. The results showed that the customers’ preferences are influenced by age and gender and the organic rose jam is a highly appreciated product.

  5. [Comparison of soil fertility among open-pit mine reclaimed lands in Antaibao regenerated with different vegetation types].

    Science.gov (United States)

    Wang, Xiang; Li, Jin-chuan; Yue, Jian-ying; Zhou, Xiao-mei; Guo, Chun-yan; Lu, Ning; Wang, Yu-hong; Yang, Sheng-quan

    2013-09-01

    Re-vegetation is mainly applied into regeneration in opencast mine to improve the soil quality. It is very important to choose feasible vegetation types for soil restoration. In this study, three typical forest restoration types were studied at Antaibao mine, namely, Medicago sativa, mixed forests Pinus taebelaefolius-Robinia pseudoacacia-Caragana korshinskii and Elaeagnus angustifolia-Robinia pseudoacacia-Caragana korshinskii-Hipophae rhamnoides, to determine the nutrient contents and enzyme activities in different soil layers. The results showed that re-vegetation markedly increased soil nutrient contents and the enzyme activities during the restoration process. The nutrient content of soil in the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest field was significantly higher than those in other plots. It was found that the soil of the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest had the highest integrated fertility index values. In conclusion, the restoration effects of the P. zaebelaefolius-R. pseudoacacia-C. Korshinskii mixed forest was better than that of E. angustifolia-R. pseudoacacia-C. korshinskii-H. rhamnoides, while M. sativa grassland had the least effect.

  6. [Species-area and species-abundance relationships of arthropod community in various vegetation restoration areas in Zhifanggou watershed, Shaanxi province of Northwest China].

    Science.gov (United States)

    Zhang, Feng; Hong, Bo; Li, Ying-Mei; Chen, Zhi-Jie; Zhang, Shu-Lian; Zhao, Hui-Yan

    2013-02-01

    Taking the Zhifanggou watershed in Ansai, Shaanxi Province of Northwest China as a study unit, an investigation on the arthropods in 8 forest stands was conducted from 2006 to 2008, with the species-area and species-abundance relationships of the arthropods in these stands analyzed by various mathematical models. In these forest stands, the species-area relationship of the arthropods accorded with the formula S= CAm With the increase of investigation area, the species number approached to a constant, and the corresponding smallest investigation area was in the order of natural bush > natural forest > Populus davidiana+Robinia pseudoacacia forest > Hippaphae rhamnoides +Caragana mocrophylla forest> Periploca sepium forest > Hippaphae rhamnoides forest > Robinia pseudoacacia forest > Caragana mocrophylla forest, indicating that the more complex the stands, the larger the minimum area needed to be investigated. Based on sampling investigation, the species-abundance models of the arthropods in various stands were established. Lognormal distribution model (LN) was most suitable to fit the arthropod community in natural recovery stands, suggesting that in the arthropod community, there were more species with medial individual amount and fewer abundant species and rare species, and no obvious dominant species. LogCauchy distribution model (LC) was most suitable to fit the arthropod community in mixed and pure stands. As compared with natural recovery stand, mixed and pure stands had more abundant and rare species, and more dominant species.

  7. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage.

    Science.gov (United States)

    Istrati, D; Lacatusu, I; Bordei, N; Badea, G; Oprea, O; Stefan, L M; Stan, R; Badea, N; Meghea, A

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to -45mV) and displayed average sizes of 70nm to 140nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract-bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Surface-enhanced Raman spectroscopy studies of yellow organic dyestuffs and lake pigments in oil paint.

    Science.gov (United States)

    Mayhew, Hannah E; Fabian, David M; Svoboda, Shelley A; Wustholz, Kristin L

    2013-08-21

    Identifying natural, organic dyes and pigments is important for the conservation, preservation, and historical interpretation of works of art. Although previous SERS studies have demonstrated high sensitivity and selectivity for red lake pigments using various pretreatment conditions, corresponding investigations of yellow lake pigments and paints are relatively sparse. Here, surface-enhanced Raman scattering (SERS) spectroscopy is used to identify a variety of yellow organic dyestuffs and lake pigments in oil paint. High-quality SERS spectra of yellow dyestuffs (i.e., turmeric, old fustic, Buckthorn berries) and corresponding paints could be obtained with or without sample pretreatment using microliter quantities of HCl and methanol at room temperature. However, the SERS spectra of yellow lake pigments (i.e., Stil de Grain, Reseda lake) and their corresponding oil paints were only observed upon sample pretreatment. Ultimately, we demonstrate a reliable sample treatment protocol for SERS-based identification of turmeric, old fustic, Buckthorn berries, Stil de Grain, and Reseda lake as well as for microscopic samples of the corresponding oil paints.

  9. NUTRIENTS ACCUMULATION IN FRUITS OF BERRY SPECIES

    Directory of Open Access Journals (Sweden)

    Sava Parascovia

    2015-12-01

    Full Text Available As a result of observations and biochemical analysis of the berries made during the years 2013-2014 in Republic of Moldova, it was found that bacciferous species included in the study had a wide spectrum of colors, from white, orange, red to blue and black. The possibility to extend the consumption of fresh berries is because these species ripen in series starting with honeysuckle in May, then one by one until September: strawberry, raspberry, currant, gooseberry, barberry, jostaberry, blueberry, sea buckthorn, blackberry, chokeberry, guilder rose. Chokeberries accumulated the highest average amount of soluble solids - 18.02%, jostaberries highlighted with the highest average amount of accumulated sugars - 9.56%. Red currant highlighted with the highest acidity - 5.22%, while blueberries with low acidity - 1.43%. Rosehip has accumulated the highest average amount of tannins and coloring substances - 546.65 mg%. Rosehip berries accumulated the highest amount of vitamin C average - 292.38 mg%, the black currant - 179.69 mg%, jostaberry - 112.86 mg%, and sea buckthorn - 96.7 mg%. The highest average values of the coefficient sugar / acid certifying good qualities were found in: blueberries - 4.39, guelder rose - 3.93, gooseberry - 3.55.

  10. “SEABUCKTHORNOLOGY” A POSSIBLE NEW INTERDISCIPLINARY SCIENCE

    Directory of Open Access Journals (Sweden)

    Angel PROOROCU

    2015-04-01

    Full Text Available The purpose of the paper was to present a critical overview on the main opinions and research results on "seabuckthornology" as a new interdisciplinary science, as mentioned on various international conferences and other events hold in different countries where the author took part and expressed his opinions as expert in the field. The current opinion of many experts is that the sea buckthorn is the result of a long hard work in the field of research, practice, landscape architecture, production, soil science, animal and human health. It is an important plant of the 3rd millennium. The only problem many experts are facing is the fact that it is very difficult to put in order all multidisciplinary information from Botany, Geology, Marketing, Medicine, Biochemistry, Agronomy, Management etc. The solution is the elaboration of a statute of the interdisciplinary new science "seabuckthornology" and the creation of a multilingual data base, which should be updated permanently as at any moment a manufacturer having sea buckthorn oil production to find many offers from the entire world, obtaining all parameters and prices in few minutes, the address, fax, e-mail, phone number etc. In the actual world crises, a scientifically aboard of seabuckthorn may be a solution to health and environmental problems.

  11. Mud-plastered granary-baskets at a Celtic Oppidum near Čarnok (Vojvodina, Serbia

    Directory of Open Access Journals (Sweden)

    Medović Aleksandar

    2011-01-01

    Full Text Available In the Celtic Oppidum near Čarnok (Bačka, Vojvodina, Serbia remains of four mud-plastered granarybaskets were discovered. They are all dated to the period Gomolava VI-B (first half of the 1st century B.C.. Hulled barley and broomcorn millet were stored in the granary-baskets. The mesh of the granary-baskets was composed of young branches of oak tree (Quercus, common English elm tree (Ulmus cf. campestris L., poplar / willow (Populus / Salix, purgin buckthorn (Rhamnus cf. cathartica L., wayfaring tree (Viburnum cf. lantana L., spindle tree (Euonymus cf. europaeus L., barberry (Berberis vulgaris L. and a Pomoidae-tree. At least four different tree taxa were used for basket making. Construction of two-thousand-year old mud-plastered granary-baskets from the Pannonian plain is very similar to that of traditional granaries in some present-day villages in Africa. Additionally, one non-plastered basket was also discovered at Oppidum. It was build out of maple (Acer, barberry, buckthorn (Rhamnus and branches of a pomaceous fruits tree (Pomoidae. .

  12. Woody invasions of urban trails and the changing face of urban forests in the great plains, USA

    Science.gov (United States)

    Nemec, K.T.; Allen, Craig R.; Alai, A.; Clements, G.; Kessler, A.C.; Kinsell, T.; Major, A.; Stephen, B.J.

    2011-01-01

    Corridors such as roads and trails can facilitate invasions by non-native plant species. The open, disturbed habitat associated with corridors provides favorable growing conditions for many non-native plant species. Bike trails are a corridor system common to many urban areas that have not been studied for their potential role in plant invasions. We sampled five linear segments of urban forest along bike trails in Lincoln, Nebraska to assess the invasion of woody non-native species relative to corridors and to assess the composition of these urban forests. The most abundant plant species were generally native species, but five non-native species were also present: white mulberry (Morus alba), common buckthorn (Rhamnus cathartica), tree-of-heaven (Ailanthus altissima), honeysuckle (Lonicera spp.) and elm (Ulmus spp.). The distribution of two of the woody species sampled, common buckthorn and honeysuckle, significantly decreased with increasing distance from a source patch of vegetation (P = 0.031 and 0.030). These linear habitats are being invaded by non-native tree and shrub species, which may change the structure of these urban forest corridors. If non-native woody plant species become abundant in the future, they may homogenize the plant community and reduce native biodiversity in these areas. ?? 2011 American Midland Naturalist.

  13. Finding of pesticides in fashionable fruit juices by LC-MS/MS and GC-MS/MS.

    Science.gov (United States)

    Tran, Kevin; Eide, David; Nickols, Susan M; Cromer, Michele R; Sabaa-Srur, Armando; Smith, Robert E

    2012-10-15

    Products labelled as containing extracts from two mushrooms (cordyceps plus reishi) and the juices from açaí, goji, mangosteen, noni, pomegranate, and sea buckthorn have been analysed for 174 different pesticides, using the validated QuEChERS method for sample preparation and electrospray LC-MS/MS in the positive ion mode for analysis. Pesticides were found in 10 of the 21 samples analysed. Most pesticides found were below the tolerance levels (1-6 μg/g, depending on the pesticide), but some were not. This included boscalid, dimethomorph, iprovalicarb, pyridaben, pyrimethanil, and imazalil, for which there is no tolerance reported or zero tolerance in any fruit. However, genuine açaí that was harvested in the state of Pará and lyophilised in Rio de Janeiro had no detectable pesticides, when analysed by both LC-MS/MS and GC-MS/MS, which can detect 213 more pesticides and industrial chemicals. Likewise no pesticides were found in one sample each of cordyceps plus reishi, sea buckthorn and noni. Published by Elsevier Ltd.

  14. Effect of wood ash and K-fertilization on {sup 137}Cs uptake by selected forest plants

    Energy Technology Data Exchange (ETDEWEB)

    Mandro, Yrii N. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Rosen, Klas [Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden); Vinichuk, Mykhailo M. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden)

    2014-07-01

    Accumulation {sup 137}Cs by different forest plants and trees after fertilization of soil with potassium and wood ash ({sup 137}Cs-contaminated and non-contaminated) in forest ecosystems of Ukraine contaminated by radionuclides after Chernobyl accident in 1986 was studied. Experiment is performed in Bazar forestry, Zhytomyr region, Ukraine, located about 70 km (51 deg. 5'35'' N, 29 deg. 18'56'' E) from Chernobyl NPP. Potassium fertilizer (KCl, wooden ash (Ash), and {sup 137}Cs-contaminated ash ({sup 137}CsAsh) in different combinations) were spread on the forest floor in April 2012 at a rate corresponding 100 kg/ha potassium. The experiment layout was as follows: 1- Control (no fertilizers were applied), 2- KCl, 3- Ash + KCl, 4- Ash + {sup 137}CsAsh, 5- Ash, 6- {sup 137}CsAsh + KCl. Samples (leaves and annual shoots) of blueberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), rowan (Sorbus aucuparia), birch (Betula), buckthorn (Frangula) and oak (Quercus robur) and also mushrooms (fruit bodies of Russula, Lactarius, Cantharellus, Collybia etc.) and berries (blueberry and cowberry) were taken monthly from each treatment. Samples were measured for {sup 137}Cs with calibrated HPGe detectors. The results of the first year studies show variation of Transfer Factors (TF) for different plants and for the same plants on different treatments. The effect of fertilization was found for blueberry on Ash-applicated (TF = 0.0085 ± 0.0025), {sup 137}CsAsh + KCl-applicated (TF = 0.0105 ± 0.0060) and Ash + KCl-applicated (TF = 0.0123 ± 0.0058) treatments compared to Control (TF = 0.0163 ± 0.0092). Also good effect for rowan on Ash + KCl-applicated treatment (TF = 0.0067 ± 0.0024) compared to Control (TF = 0.0100 ± 0.0064). Effect was less obvious for birch on Ash + KCl-applicated treatment and for cowberry on Ash + KCl-applicated treatment. There was not found an obvious effect of fertilization for buckthorn. Positive effect of

  15. Analysis of vitamin C in selected medicinal plants

    International Nuclear Information System (INIS)

    Hussain, I.

    2011-01-01

    Vitamin C or ascorbic acid, a very useful constituent of redox mechanism is used in medicine and also added in manufactured food for anti-oxidation. A UV-spectrophotometric method was used for the determination of Vitamin C in 4 different medicinal plants. High amount of Vitamin C 160 mg/100 g was found in Citrulus colcocynthis, followed by Hippophae rhamonides oil 136.1 mg/100g. A relatively low concentration of Vitamin C was recorded in Glycyrhiza glabra 56.2 mg/100g and Withinia somnifera 51.50 mg/100 g. The presence of high concentration of Vitamin C in selected medicinal plants might be responsible for their therapeutic effects and uses in the traditional system of medicine. (author)

  16. Profiling and Quantification of Regioisomeric Caffeoyl Glucoses in Berry Fruits.

    Science.gov (United States)

    Patras, Maria A; Jaiswal, Rakesh; McDougall, Gordon J; Kuhnert, Nikolai

    2018-02-07

    On the basis of a recently developed tandem mass spectrometry-based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected berry fruits were profiled for their caffeoyl glucose ester content. Fresh edible berries profiled, including strawberries, raspberries, blueberries, blackberries, red currant, black currant, lingonberries, gooseberries, and juices of elderberries, goji berries, chokeberries, cranberries, açai berries, sea buckthorn berries, Montmorency sour cherries, and pomegranates, were investigated. 1-Caffeoyl glucose was found to be the predominant isomer in the majority of samples, with further profiling revealing the presence of additional hydroxycinnamoyl glucose esters and O-glycosides with p-coumaroyl, feruloyl, and sinapoyl substituents. A quantitative liquid chromatography-mass spectrometry-based method was developed and validated, and all caffeoyl glucose isomers were quantified for the first time in edible berries.

  17. [Hygienic significance of patulin in food. 2. Occurrence of patulin in fruit and vegetables].

    Science.gov (United States)

    Thurm, V; Paul, P; Koch, C E

    1979-01-01

    The analyses of more than 200 samples of various foods of plant origin showed that patulin was contained in 36% of the fresh and canned fruits infested with mould, and in 7% of the vegetables. Besides apples, pears, plums, peaches and tomatoes contained also patulin. In organoleptically impeccable fruit juices, the contamination rates were 40% (for apple juice) and 16% (for the other juices, such as sour cherry, currant, sea buckthorn juices). The patulin content varied from 20 to 200 microgram/l, the mean value being 80 microgram/l. It ranged from 0.1 to 5 microgram/g in apples and sterile apple preserves. The authors discuss the hygienic-toxicologic significance of these findings, and suggest to include patulin in the examination of foods for mycotoxins, stipulating a permissible value.

  18. Children's acceptance learning of New Nordic components and potential challenges

    DEFF Research Database (Denmark)

    Hartvig, Ditte Luise

    that repeated exposure as well as food engagement constitute efficient methods to enhance the acceptance of Nordic foods. Furthermore the importance of follow-up tests and initial liking was highlighted. Many different factors affect acceptance and acceptance learning of food products, some of those may even......It has been suggested that dietary recommendations should be tailored to regional conditions to bridge gastronomi, health and sustainability. The New Nordic diet (NND) has been defined as part of the OPUS project:”Optimal well-being, development and health of school children through a New Nordic......’s food preferences. In the first part of the project it was investigated how a five week intervention with Nordic foods and food engagement affected the acceptance of sea-buckthorn berry products, not included in the intervention. The effect of the intervention was compared to the effect of eight product...

  19. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage

    International Nuclear Information System (INIS)

    Istrati, D.; Lacatusu, I.; Bordei, N.; Badea, G.; Oprea, O.; Stefan, L.M.; Stan, R.; Badea, N.; Meghea, A.

    2016-01-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to − 45 mV) and displayed average sizes of 70 nm to 140 nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract–bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. - Highlights: • Safety phyto-mediated nanostructured carriers (NLC) based on two kinds of bioactives • Carrot extract incorporation into nanostructured carriers ranged from 78 to 88.3%. • High antioxidant activity of NLC by scavenging up to 98% free oxygen radicals • Extract entrapment represents a viable way for an equilibrated release of carotenoids. • Remarkable regenerative effect of L929 cell, with a proliferation of 133.4%

  20. Effect of Plant Antimicrobial Agents Containing Marinades on Storage Stability and Microbiological Quality of Broiler Chicken Cuts Packed with Modified Atmosphere Packaging.

    Science.gov (United States)

    Alakomi, H-L; Maukonen, J; Honkapää, K; Storgårds, E; Quirin, K-W; Yang, B; Saarela, M

    2017-10-01

    The food industry, including the meat industry, is currently looking for natural preservatives to prevent the growth of harmful microbes in foods. The potential of plant-derived antimicrobial extracts to increase the shelf life and to delay the microbiological spoilage of marinated broiler chicken cuts in modified atmosphere packages during cold storage was investigated in this study. We evaluated the impact of aqueous ethanolic extracts of Finnish sea buckthorn berries and lingonberries and supercritical CO 2 -extracted herbal extracts from an antimicrobial blend and oregano leaves on the shelf life of broiler meat. The commercial antimicrobial blend extract and the oregano extract inhibited the growth of lactic acid bacteria (LAB) and Brochothrix thermosphacta in the marinated samples. The antimicrobial blend extract also reduced the growth of psychrotrophic aerobic bacteria, whereas the sea buckthorn and lingonberry extracts did not. Only minor antimicrobial activity against Enterobacteriaceae by all the extracts was observed. Plate count analysis, denaturing gradient gel electrophoresis, and quantitative real-time PCR indicated that LAB, which are the major spoilage group in marinated modified atmosphere-packaged poultry products, were not significantly affected by the berry extracts studied. During this shelf-life study, LAB isolates of Lactobacillus and Leuconostoc were identified in the marinated samples. Antimicrobial blends and oregano leaf extracts can act as antimicrobial agents in marinade blends, although tailoring of the dose is needed because of their strong taste. Further studies for exploiting synergistic effects of plant extracts could contribute to the development of potential and more effective antimicrobial blends. Studies are needed in meat matrices and in product applications to demonstrate the efficacy of these compounds.

  1. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage

    Energy Technology Data Exchange (ETDEWEB)

    Istrati, D.; Lacatusu, I.; Bordei, N.; Badea, G.; Oprea, O. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Stefan, L.M. [National Institute of Research and Development for Biological Sciences, Splaiul Independentei Street No. 296, 060031 Bucharest (Romania); Stan, R. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Badea, N., E-mail: nicoleta.badea@gmail.com [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Meghea, A. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania)

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to − 45 mV) and displayed average sizes of 70 nm to 140 nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract–bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. - Highlights: • Safety phyto-mediated nanostructured carriers (NLC) based on two kinds of bioactives • Carrot extract incorporation into nanostructured carriers ranged from 78 to 88.3%. • High antioxidant activity of NLC by scavenging up to 98% free oxygen radicals • Extract entrapment represents a viable way for an equilibrated release of carotenoids. • Remarkable regenerative effect of L929 cell, with a proliferation of 133.4%.

  2. [Evaluation of antioxidant properties of enriched bakery products in experiment on laboratory animals].

    Science.gov (United States)

    Nilova, L P; Pilipenko, T V

    2016-01-01

    The purpose was to study the effect of enriched bakery products in the diet of rats on indicators of prooxidant-antioxidant system of blood serum. Experiment was carried out on male Wistar rats with initial weight 140-180 g. After a quarantine during the preparatory period rats for 14 days were accustomed to the partial (50%) replacement of the standard diet by bakery products with standard compound­ing. Then, 7 groups of rats were formed: the 1st group of rats (control group, n=10) continued to receive bakery products of a standard composition; groups with the 2nd on 7th (experimental, n=8 in everyone) received enriched bakery products: the 2nd group - with blueberry powder; the 3rd group - with mountain ash powder; the 4th group - with sea-buckthorn powder; the 5th group - with flour of a pine nut; the 6th group - with rice bran oil; the 7th group - with pumpkin oil. The intensity of free radical oxidation and antioxidant activity (by chemiluminescence method), activity of superoxide dismutase and level of secondary oxidation products reacted with thiobarbituric acid (by spectrophotometry) were monitored in rat blood serum. It has been shown that the use of bakery products with different compounding in the animal diet had different effects on indicators of prooxidant-antioxidant system of blood serum. Bakery products containing sea buckthorn pomace powder, flour of pine nut and rice bran oil reduced intensity of free radical oxidation in rat blood serum by 36.0, 24.6 and 18.8%, respectively. It is suggested that bakery products containing flour of pine nut products brake a free radical oxidation in rat blood serum in case of simultaneous content of natural antioxidants and melanoidins. The anthocyanins of powder from blueberry berries can render antioxidant effect and slow down formation of by-products of oxidation. No statistically significant change on indicators of prooxidant-antioxidant system of blood serum of rats treated with bakery products with rowan

  3. Correlation of antioxidant activity of dried berry infusions with the polyphenols and selected microelements contents

    Directory of Open Access Journals (Sweden)

    M. M. Bratu

    2018-04-01

    Full Text Available Antioxidant activity was measured by ferric reducing ability of plasma (FRAP assay in seven types of infusions prepared from commercial dried berry fruit products: Rosa canina, Vaccinium vitis-idaea, Hiphophae rhamnoides, Hibiscus sabdariffa and three fruit mixtures. Total polyphenols (TP, total anthocyanins and the polyphenolic compounds were determined by HPLC equipped with diode array detector. To estimate the amount of elements released from fruits into the water extracts, levels of Fe, Mn, Zn and Cu in dried samples and in infusions were determined by flame atomic absorption spectrometry. The correlation between polyphenols content and the antioxidant activities and the microelements in the infusions and the antioxidant activities were estimated using the Pearson’s correlation test. The results showed a high, positive and significant correlation (r = 0.9465 between the FRAP values and TP content, meaning that the concentration of phenolic compounds may be a good indicator of the reducing capacity in the infusions. Correlations varied (positive, negative and weak between antioxidant and mineral extractability of berry infusions. Among the polyphenolic compounds, gallic acid contributed particularly to the antioxidant capacity of the studied samples (r = 0.563. The correlation of antioxidants, total polyphenols with mineral extractability showed the influence of antioxidant compound on mineral bioavailability.

  4. Woody vegetation, fuel and fire track the melting of the Scandinavian ice-sheet before 9500 cal yr BP

    Science.gov (United States)

    Carcaillet, Christopher; Hörnberg, Greger; Zackrisson, Olle

    2012-11-01

    New studies indicate the presence of early Holocene ice-free areas far north in Scandinavia. Post-glacial fire and vegetation were investigated based on sedimentary charcoal and pollen from two small lakes in northern Sweden. Accumulation of organic sediment started around 10,900 and 9200 cal yr BP, showing that both lake valleys were ice-free extremely early given their northerly location. Fire events started after 9600 cal yr BP and became less common around the '8.2-ka event'. Woody vegetation provided fuel that contributed to fires. The first vegetation in our pollen record consisted of Hippophae, Dryas, grasses and sedges. Subsequently broadleaved trees (Betula, Salix) increased in abundance and later Pinus, Alnus, ferns and Lycopodium characterized the vegetation. Pollen from Larix, Picea and Malus were also found. The change in vegetation composition was synchronous with the decrease in lake-water pH in the region, indicating ecosystem-scale processes; this occurred during a period of net global and regional warming. The changes in fire frequency and vegetation appear independent of regional trends in precipitation. The reconstructed fire history and vegetation support the scenario of early ice-free areas far north in Scandinavia during early Holocene warming, creating favorable conditions for woody plants and wildfires.

  5. A study of the development of bio-energy resources and the status of eco-society in China

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xia; Huang, Yongmei; Gong, Jirui [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Zhang, Xinshi [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Institute of Botany, CAS, Beijing 100093 (China)

    2010-11-15

    Industrialization of bio-energy relies on the supply of resources on a large scale. The theoretical biomass resources could reach 2.61-3.51 billion tce (tons of coal equivalent)/a in China, while the available feedstock is about 440-640 million tce/a, however, among this only 1.5-2.5% has been transferred into energy at present. Marginal land utilization has great prospects of supplying bio-energy resources in China, with co-benefits, such as carbon sequestration, water/soil conservation, and wind erosion protection. There is a large area of marginal land in China, especially in northern China, including about 263 million ha of desertification land, 173 million ha of sand-land, and 17 million ha of salinizatin land. The plant species suitable to be grown in marginal lands, including some species in Salix, Hippophae, Tamarix, Caragana, and Prunus is also abundant Biomass feedstock in marginal lands would be 100 million tce/a in 2020, and 200 million tce/a in 2050. As a result, a win-win situation of eco-society and bio-energy development could be realized, with an expected 4-5% reduction of total CO{sub 2} emission in China in 2020-2050. Although much progress has been made in the field of bio-energy research in China, yet significant efforts should be taken in the future to fulfill large-scale industrialization of bio-energy. (author)

  6. Phytochemicals with radioprotection and radio-sensitizing potential

    Energy Technology Data Exchange (ETDEWEB)

    Tripathi, Yamini B., E-mail: yaminiok@yahoo.com [Dept. of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi (India)

    2012-07-01

    Ionizing radiation induces DNA damage and are harmful to mankind. They act through free radical generation, which target the double bonds of all cellular macromolecules. The radiation damage may be classified as probabilistic or deterministic, depending on the dose of radiation exposure. Since radiation affects multiple organs so those drugs which protect many organs, would be more beneficial. In this process herbal extracts, which are cocktail of several phyto-chemicals, would be more promising. Initially sulphur containing bio-molecules were identified as radio-protector, but now many secondary metabolites from plant kingdom, have been reported to be radio-protective. They have different mechanism of action, but most of them either prevent the FR induced DNA damage or accelerate the DNA repair process. Aminofostine, WR-2721, 159243, 2926 are some of the examples. However they have limited use because of associated cytotoxicity. Eicosanoids, topoisornerase inhibitors (e.g. camptothecin, topotecan), and the hypoxia-activated anthraquinone AQ4N have shown radioprotecting potential. Several plant products, derived from Tulsi, Vinca alkaloids, taxans, turmeric, Rubia cordifolia, Semecarpus anacardium and several plants rich in polyphenols and flavones have shown hemotherapeutic potential. Similarly, Hippophae, rhodiola imbricata, Podophyllum hexandrum, Ocimum sancturn, Plumbago zeylanica etc have shown radioprotection. Rubia cordifolia has shown both chemotherapeutic and radioprotective property in rats and A-431 cells. Similarly Semecarpus anacardium extract has shown cell cycle arrest in DU-145 cells. (author)

  7. [Species-associated differences in foliage-root coupling soil-reinforcement and anti-erosion].

    Science.gov (United States)

    Liu, Fu-quan; Liu, Jing; Nao, Min; Yao, Xi-jun; Zheng, Yong-gang; Li, You-fang; Su, Yu; Wang, Chen-jia

    2015-02-01

    This paper took four kinds of common soil and water conservation plants of the study area, Caragana microphylla, Salix psammophila, Artemisia sphaerocephala and Hippophae rhamnides at ages of 4 as the research object. Thirteen indicators, i.e., single shrub to reduce wind velocity ration, shelterbelt reducing wind velocity ration, community reducing wind velocity ration, taproot tensile strength, representative root constitutive properties, representative root elasticity modulus, lateral root branch tensile strength, accumulative surface area, root-soil interface sheer strength, interface friction coefficient, accumulative root length, root-soil composite cohesive, root-soil composite equivalent friction angle, reflecting the characteristics of windbreak and roots, were chose to evaluate the differences of foliage-root coupling soil-reinforcement and anti-erosion among four kinds of plants by analytic hierarchy process (AHP) under the condition of spring gale and summer rainstorm, respectively. The results showed the anti-erosion index of foliage-root coupling was in the sequence of S. psammophila (0.841) > C. microphylla (0.454) > A. sphaerocephala (-0.466) > H. rhamnides (-0.829) in spring gale, and C. microphylla (0.841) > S. psammophila (0. 474) > A. sphaerocephala (-0.470) > H. rhamnides (-0.844) in summer rainstorm. S. psammophila could be regarded as one of the most important windbreak and anti-erosion species, while C. microphylla could be the most valuable soil and water conservation plant for the study area.

  8. Phytochemicals with radioprotection and radio-sensitizing potential

    International Nuclear Information System (INIS)

    Tripathi, Yamini B.

    2012-01-01

    Ionizing radiation induces DNA damage and are harmful to mankind. They act through free radical generation, which target the double bonds of all cellular macromolecules. The radiation damage may be classified as probabilistic or deterministic, depending on the dose of radiation exposure. Since radiation affects multiple organs so those drugs which protect many organs, would be more beneficial. In this process herbal extracts, which are cocktail of several phyto-chemicals, would be more promising. Initially sulphur containing bio-molecules were identified as radio-protector, but now many secondary metabolites from plant kingdom, have been reported to be radio-protective. They have different mechanism of action, but most of them either prevent the FR induced DNA damage or accelerate the DNA repair process. Aminofostine, WR-2721, 159243, 2926 are some of the examples. However they have limited use because of associated cytotoxicity. Eicosanoids, topoisornerase inhibitors (e.g. camptothecin, topotecan), and the hypoxia-activated anthraquinone AQ4N have shown radioprotecting potential. Several plant products, derived from Tulsi, Vinca alkaloids, taxans, turmeric, Rubia cordifolia, Semecarpus anacardium and several plants rich in polyphenols and flavones have shown hemotherapeutic potential. Similarly, Hippophae, rhodiola imbricata, Podophyllum hexandrum, Ocimum sancturn, Plumbago zeylanica etc have shown radioprotection. Rubia cordifolia has shown both chemotherapeutic and radioprotective property in rats and A-431 cells. Similarly Semecarpus anacardium extract has shown cell cycle arrest in DU-145 cells. (author)

  9. Salt stress-induced changes in antioxidative defense system and proteome profiles of salt-tolerant and sensitive Frankia strains.

    Science.gov (United States)

    Srivastava, Amrita; Singh, Anumeha; Singh, Satya S; Mishra, Arun K

    2017-04-16

    An appreciation of comparative microbial survival is most easily done while evaluating their adaptive strategies during stress. In the present experiment, antioxidative and whole cell proteome variations based on spectrophotometric analysis and SDS-PAGE and 2-dimensional gel electrophoresis have been analysed among salt-tolerant and salt-sensitive Frankia strains. This is the first report of proteomic basis underlying salt tolerance in these newly isolated Frankia strains from Hippophae salicifolia D. Don. Salt-tolerant strain HsIi10 shows higher increment in the contents of superoxide dismutase, catalase and ascorbate peroxidase as compared to salt-sensitive strain HsIi8. Differential 2-DGE profile has revealed differential profiles for salt-tolerant and salt-sensitive strains. Proteomic confirmation of salt tolerance in the strains with inbuilt efficiency of thriving in nitrogen-deficient locales is a definite advantage for these microbes. This would be equally beneficial for improvement of soil nitrogen status. Efficient protein regulation in HsIi10 suggests further exploration for its potential use as biofertilizer in saline soils.

  10. Ethnomedicine in Himalaya: a case study from Dolpa, Humla, Jumla and Mustang districts of Nepal

    Directory of Open Access Journals (Sweden)

    Kshhetri Hari B

    2006-06-01

    Full Text Available Abstract Traditional plant use in Nepal has been documented for millennia. The importance of plants as medicine has not diminished in any way in recent times, and traditional medicines are still the most important health care source for the vast majority of the population. This paper examines the ethnobotany and traditional use of plants extracted from the vulnerable alpine zone in the Dolpa, Humla, Jumla and Mustang districts of Nepal. The results of this ethnobotanical study indicate that a very large number of plant species is used as traditional medicines. There were 107, 59, 44 and 166 species of ethnomedicinal importance in surveyed areas of Dolpa, Humla, Jumla and Mustang district respectively. Of these, 84 common species, used at least in two districts, were selected to enumerate their ethnomedicinal properties. The 84 species belonged to 75 genera and 39 families. The commonest species in this pharmacopoeia were: Allium wallichii, Cordyceps sinensis, Dactylorhiza hatagirea, and Rheum australe. A total of 21 species were most common in three districts and 59 in two districts. The genera Aconitum, Allium, Arisaema, Berberis, Corydalis, Gentiana, Hippophae, Juniperus and Rhododendron each possessed two species with ethnomedicinal use. Labiatae was the most medicinally important family with five species used, followed by Araceae, Compositae, Liliaceae, Polygonaceae, Ranunculaceae, Scrophulariaceae and Umbelliferae, each contributing four species.

  11. [Difference of anti-fracture mechanical characteristics between lateral-root branches and adjacent upper straight roots of four plant species in vigorous growth period].

    Science.gov (United States)

    Liu, Peng-fei; Liu, Jing; Zhu, Hong-hui; Zhang, Xin; Zhang, Ge; Li, You-fang; Su, Yu; Wang, Chen-jia

    2016-01-01

    Taking four plant species, Caragana korshinskii, Salix psammophila, Hippophae rhamnides and Artemisia sphaerocephala, which were 3-4 years old and in vigorous growth period, as test materials, the anti-fracture forces of lateral-root branches and adjacent upper straight roots were measured with the self-made fixture and the instrument of TY 8000. The lateral-root branches were vital and the diameters were 1-4 mm. The results showed that the anti-fracture force and anti-fracture strength of lateral-root branches were lesser than those of the adjacent upper straight roots even though the average diameter of lateral-root branches was greater. The ratios of anti-fracture strength of lateral-root branches to the adjacent upper straight roots were 71.5% for C. korshinskii, 62.9% for S. psammophila, 45.4% for H. rhamnides and 35.4% for A. sphaerocephala. For the four plants, the anti-fracture force positively correlated with the diameter in a power function, while the anti-fracture strength negatively correlated with diameter in a power function. The anti-fracture strengths of lateral-root branches and adjacent upper straight roots for the four species followed the sequence of C. korshinskii (33.66 and 47.06 MPa) > S. psammophila (17.31 and 27.54 MPa) > H. rhamnides (3.97 and 8.75 MPa) > A. sphaerphala (2.18 and 6.15 MPa).

  12. Avoidance of nonhost plants by a bark beetle, Pityogenes bidentatus, in a forest of odors

    Science.gov (United States)

    Byers, John A.; Zhang, Qing-He; Birgersson, Göran

    The bark beetle, Pityogenes bidentatus (Coleoptera: Scolytidae), searches in mixed conifer and deciduous forests of northern Europe for suitable branches of its host, Scots pine (Pinus sylvestris). We tested whether odors from several diverse nonhost trees and plants common in the habitat (e.g., mountain ash, Sorbus aucuparia; oak, Quercus robur; alder buckthorn, Frangula alnus; blueberry, Vaccinium myrtillus; raspberry, Rubus idaeus; and grass, Deschampsia flexuosa) would reduce the attraction of the bark beetle to traps releasing its aggregation pheromone components in the field. Volatiles from the leaves or bark of each of these plants significantly reduced the attraction of the beetles to their pheromone. Odors collected from these nonhosts and analyzed by GC/MS contained monoterpenes, sesquiterpenes, and ``green-leaf'' alcohols, several of which (e.g., 1-octene-3-ol and β-caryophyllene) reduced the attraction to pheromone in the field and elicited electroantennographic responses. In the laboratory, reproduction by the beetle was marginal in nonhost Norway spruce, Picea abies, and was absent in the other nonhost trees. Olfactory avoidance of unsuitable nonhosts may have evolved due to advantages in avoiding mistakes during host selection.

  13. Botanicals and Phosphonate Show Potential to Replace Copper for Control of Potato Late Blight

    Directory of Open Access Journals (Sweden)

    Hans-Rudolf Forrer

    2017-11-01

    Full Text Available Potato late blight (PLB caused by Phytophthora infestans (Pi is the most harmful disease in potato production worldwide. In organic farming, copper is used despite its persistence in soil and toxicity to soil organisms. To replace copper, suspensions of powders from three promising botanicals, including bark of buckthorn (Frangula alnus, FA, roots of medicinal rhubarb (Rheum palmatum and galls of the nutgall tree (Galla chinensis, were tested in multi-year field experiments. The current study shows for the first time that botanicals could replace copper under field conditions and best PLB reduction on leaves was achieved with FA, reaching a level close to that of 2 to 3 kg copper per hectare and year. Better results than with copper were achieved with Phosfik® (Ph, a phosphonate-based product. For both FA and Ph, the mode of action is based on induced resistance, for Ph also on direct fungicidal effects. A disadvantage of Ph is the accumulation of residues in potato tubers. Nevertheless, two to three applications with 2 to 3 L/ha of Ph would be feasible to not exceed a minimal risk level (MLR of 20 mg/kg of phosphorous acid as proposed by the European Food Safety Authority. Due to an excellent environmental profile and a complex mode of action counteracting Pi resistance, phosphonate-based products would be most suitable for sustainable PLB management in integrated pest management (IPM programmes.

  14. Skin Penetration Enhancement by Natural Oils for Dihydroquercetin Delivery.

    Science.gov (United States)

    Čižinauskas, Vytis; Elie, Nicolas; Brunelle, Alain; Briedis, Vitalis

    2017-09-12

    Natural oils are commonly used in topical pharmaceutical formulations as emulsifiers, stabilizers or solubility enhancers. They are presented as safe and inert components, mainly used for formulation purposes. It is confirmed that natural oils can affect the skin penetration of various substances. Fatty acids are mainly responsible for this effect. Current understanding lacks reliable scientific data on penetration of natural oils into the skin and their skin penetration enhancement potential. In the current study, fatty acid content analysis was used to determine the principal fatty acids in soybean, olive, avocado, sea-buckthorn pulp, raspberry seed and coconut oils. Time of flight secondary ion mass spectrometry bioimaging was used to determine the distribution of these fatty acids in human skin ex vivo after application of the oils. Skin penetration enhancement ratios were determined for a perspective antioxidant compound dihydroquercetin. The results demonstrated skin penetration of fatty acids from all oils tested. Only soybean and olive oils significantly increased the skin distribution of dihydroquercetin and can be used as skin penetration enhancers. However, no correlation can be determined between the fatty acids' composition and skin penetration enhancement using currently available methodological approaches. This indicates that potential chemical penetration enhancement should be evaluated during formulation of topically applied products containing natural oils.

  15. Acceptance of Nordic snack bars in children aged 8–11 years

    Directory of Open Access Journals (Sweden)

    Anna Holmer

    2012-04-01

    Full Text Available Background: A health promoting diet is suggested to be tailored to regional circumstances to preserve the cultural diversity in eating habits, as well as contribute to more environmentally friendly eating. It may influence consumer acceptance, however, if the components of the diet differs considerably from their habitual food. Objective: This study aimed to investigate whether snack bars composed of Nordic ingredients were accepted by 8–11 year-old Danish (n=134 and Swedish (n=109 children.Design: A seven-point hedonic scale was used to measure the children's acceptance of five snack bars that varied in their composition of whole grains, berries and nuts. A preference rank ordering of the five bars was also performed. Results: The results showed that samples that were rated highest in liking and were most preferred in both countries were a kamut/pumpkin bar and an oat/cranberry bar. The sample with the lowest rating that was also least preferred was a pumpernickel/sea buckthorn bar. Flavour was the most important determinant of overall liking followed by texture, odour and appearance. Conclusions: Children's acceptances and preferences were highly influenced by the sensory characteristics of the bars, mainly flavour. In agreement with earlier studies, the novel food ingredients seemed to influence children's preferences. The Nordic snack bars may have a potential to be a snack option for Danish and Swedish school children, but repeated exposures to the products are recommended to increase children's acceptance.

  16. A review of characterization of tocotrienols from plant oils and foods.

    Science.gov (United States)

    Ahsan, Haseeb; Ahad, Amjid; Siddiqui, Waseem A

    2015-04-01

    Tocotrienols, members of the vitamin E family, are natural compounds found in a number of vegetable oils, wheat germ, barley and certain types of nuts and grains. Vegetable oils provide the best sources of these vitamin E forms, particularly palm oil and rice bran oil contain higher amounts of tocotrienols. Other sources of tocotrienols include grape fruit seed oil, oats, hazelnuts, maize, olive oil, buckthorn berry, rye, flax seed oil, poppy seed oil and sunflower oil. Tocotrienols are of four types, viz. alpha (α), beta (β), gamma (γ) and delta (δ). Unlike tocopherols, tocotrienols are unsaturated and possess an isoprenoid side chain. A number of researchers have developed methods for the extraction, analysis, identification and quantification of different types of vitamin E compounds. This article constitutes an in-depth review of the chemistry and extraction of the unsaturated vitamin E derivatives, tocotrienols, from various sources using different methods. This review article lists the different techniques that are used in the characterization and purification of tocotrienols such as soxhlet and solid-liquid extractions, saponification method, chromatography (thin layer, column chromatography, gas chromatography, supercritical fluid, high performance), capillary electrochromatography and mass spectrometry. Some of the methods described were able to identify one form or type while others could analyse all the analogues of tocotrienol molecules. Hence, this article will be helpful in understanding the various methods used in the characterization of this lesser known vitamin E variant.

  17. Comprehensive treatment of generalized parodontitis.

    Science.gov (United States)

    Mdinaradze, N

    2006-06-01

    The complexity of pathogenesis of periodontitis makes the use of comprehensive treatment necessary. We observed 60 patients, 20 women and 40 men among them aged from 20 to 50. In the course of parodontitis, we determined the intensity of inflammatory-destructive changes (Pi) in the course of periodontitis, the degree of fang denudation, the depth of gum and parodontal recesses, degree of the teeth becoming loose; we conducted the microbiological study of the oral cavity microflora and drew up an antibiotic graph. We included laser therapy in the comprehensive treatment course of generalized parodontitis. We divided the patients into two groups. The patients of the first group were treated in two stages. We used peridex, composed of chlorhexidine for antiseptic treatment of oral cavity. We used a combined solution of sage, eucalyptus, camomile and calendula to irrigate the oral cavity. At the second stage we used ointment applications with the composition of 3% indometacin, heparin, vitamins, sea-buckthorn oil and antibiotics. In the course of comprehensive treatment of the patients of the II group we included laser therapy. Our interest in the laser beam, as in the means of parodontitis treatment was stimulated by a number of properties of the laser beam and namely, its anti-inflammatory, desensitizing and antibacterial action promotes intensifying the reparation processes and does not entail any complications. The mentioned method has produced the best therapeutic results and has resulted in the reduced treatment period.

  18. Cold hardiness research on agricultural and horticultural crops in Finland

    Directory of Open Access Journals (Sweden)

    L. LINDÉN

    2008-12-01

    Full Text Available This paper represents an overview of cold hardiness research conducted on agricultural and horticultural crops, as well as on amenity plants in Finland. Inadequate freezing tolerance and/or winter hardiness often prevents introduction of new species and cultivars to Finland. Field observations on winter hardiness and more recently the results from laboratory freezing tests, have assisted breeders to select hardy genotypes. Research approaches for agricultural crops have evolved from observations on winter and frost damage to studies on molecular mechanisms of cold acclimation and freezing injury. The results of experiments on survival of winter cereals, grasses and clovers and frost tolerance of potato and turnip rape are discussed. The studies conducted on horticultural crops, including apple, strawberry, raspberry, currants, blueberry, sea buckthorn, perennial herbs as well as on ornamental trees and shrubs have included field evaluations of cultivars, or selections for winter hardiness, and studies on the effects of cultural management practices on winter survival. During the last decade detailed studies including controlled freezing tests have provided tools to assist in explanation of the underlying mechanisms of cold hardiness also in horticultural plants. ;

  19. Acceptance of Nordic snack bars in children aged 8-11 years.

    Science.gov (United States)

    Holmer, Anna; Hausner, Helene; Reinbach, Helene C; Bredie, Wender L P; Wendin, Karin

    2012-01-01

    A health promoting diet is suggested to be tailored to regional circumstances to preserve the cultural diversity in eating habits, as well as contribute to more environmentally friendly eating. It may influence consumer acceptance, however, if the components of the diet differs considerably from their habitual food. This study aimed to investigate whether snack bars composed of Nordic ingredients were accepted by 8-11 year-old Danish (n=134) and Swedish (n=109) children. A seven-point hedonic scale was used to measure the children's acceptance of five snack bars that varied in their composition of whole grains, berries and nuts. A preference rank ordering of the five bars was also performed. The results showed that samples that were rated highest in liking and were most preferred in both countries were a kamut/pumpkin bar and an oat/cranberry bar. The sample with the lowest rating that was also least preferred was a pumpernickel/sea buckthorn bar. Flavour was the most important determinant of overall liking followed by texture, odour and appearance. Children's acceptances and preferences were highly influenced by the sensory characteristics of the bars, mainly flavour. In agreement with earlier studies, the novel food ingredients seemed to influence children's preferences. The Nordic snack bars may have a potential to be a snack option for Danish and Swedish school children, but repeated exposures to the products are recommended to increase children's acceptance.

  20. Tecnology of development phytogenetic protector's andcreation of raw-material base

    International Nuclear Information System (INIS)

    Rzayev, N.R.; Guliyev, N.T

    2011-01-01

    Full tex: Recently in order to protect organisms from the of radiation effect radio protectors have been applied principally concerning to sulfur compounds, aminothioles and indolilalkins. Although the achievements obtained in the direction of synthetic medicines discovery, they can not completely replace the medicinal preparation of natural origin. That is why the study of natural biological active substances with radio protector property are of special importance.One of the goals of the project is the detection of plant species rich with anti mutagen and radio protector, the study of their cultivation technology, and the produce of natural raw material with high quality in planting condition. Flora of Azerbaijan is considered one of the richest countries for its species' richness. There are plant varieties for producing radio protector substances (essence-oil, lipid, lipoid, phenol compounds, pigment substances, proteins, vitamins and various amino acids). We have cultivated new varieties and forms of sea buckthorn, black caraway, basil, tarragons which are richer with their radio protector substances among these plants. Our aim in producing new varieties and forms is to get new varieties which rich with protector property substances, by conducting selection in condition of the cultivation without touching the balance of natural biodiversity and to protect the species with few natural resources.In the article it is intended to prepare aerosol, consisted of essence oils and lipid mixtures, for respiratory system treatment in extreme conditions having radiation threat. The plants with essence oil are potential radio protectors.A technology will be worked out for the produce of cream, protecting surrounding tissues during radiation therapy; for producing radio protector propertied oils from these plant, fruits and seeds in order to treat radioactive burns of digestive system and skin; balsam producing for prophylactic purposes; hygienic remedies such as shampoo

  1. Composition of some botanical mixtures as potential feed additives for laying hens

    Directory of Open Access Journals (Sweden)

    Varzaru Iulia

    2015-01-01

    Full Text Available The aim of this study was to assess the nutritional quality of four botanical mixtures (AFC: AFC 1 (containing red corn, pumpkin pulp and marigold, AFC 2 (containing alfalfa meal, pumpkin pulp and marigold, AFC 3 (containing kale, alfalfa meal, marigold and spinach leaves, AFC 4 (containing buckthorn, red corn, pumpkin pulp and marigold, in terms of proximate analysis (crude protein, crude fat, crude fiber, ash, amino acid (AA profile, vitamin E concentration and lutein and zeaxanthin content, in order to determine the potential of AFCs as feed additives in laying hens nutrition. The crude protein content for the analysed botanical mixtures ranged between 9.07-18.18% DM, and crude fiber between 10.41-30.83% DM. The amino acid profile of the mixture AFC 4 revealed a content of limiting essential amino acids required for laying hens: lysine 5.719% CP, methionine 1.058% CP and threonine 4.415% CP. The highest content of lutein and zeaxanthin was found in the mixture AFC 4 (66.659 mg/100 g, which also had the highest amount of vitamin E (640.93 mg/kg. With regard to safety of the botanical mixtures, lead and cadmium concentrations were determined. Concentration of lead ranged from 0.28-0.75 µg/g DM and 0.06-0.09 µg/g DM for concentration of cadmium, which was within the legislation of maximal limits of EU regulations. It can be concluded that the botanical mixture AFC 4 had the highest concentration of lutein, zeaxanthin and vitamin E, with an adequate content of essential amino acids. Furthermore, all four botanical mixtures had high amounts of xantophylls and should be tested in laying hens trials in order to establish their effects on lutein and zeaxanthin concentration in egg yolk.

  2. Berry Phenolic Antioxidants – Implications for Human Health?

    Science.gov (United States)

    Olas, Beata

    2018-01-01

    Antioxidants present in the diet may have a significant effect on the prophylaxis and progression of various diseases associated with oxidative stress. Berries contain a range of chemical compounds with antioxidant properties, including phenolic compounds. The aim of this review article is to provide an overview of the current knowledge of such phenolic antioxidants, and to discuss whether these compounds may always be natural gifts for human health, based on both in vitro and in vivo studies. It describes the antioxidant properties of fresh berries (including aronia berries, grapes, blueberries, sea buckthorn berries, strawberries and other berries) and their various products, especially juices and wines. Some papers report that these phenolic compounds may sometimes behave like prooxidants, and sometimes demonstrate both antioxidant and prooxidant activity, while others note they do not behave the same way in vitro and in vivo. However, no unwanted or toxic effects (i.e., chemical, hematological or urinary effect) have been associated with the consumption of berries or berry juices or other extracts, especially aronia berries and aronia products in vivo, and in vitro, which may suggest that the phenolic antioxidants found in berries are natural gifts for human health. However, the phenolic compound content of berries and berry products is not always well described, and further studies are required to determine the therapeutic doses of different berry products for use in future clinical studies. Moreover, further experiments are needed to understand the beneficial effects reported so far from the mechanistic point of view. Therefore, greater attention should be paid to the development of well-controlled and high-quality clinical studies in this area. PMID:29662448

  3. Dynamics of phytomass of a tree stand of the deciduous-coniferous phytocenosis in middle taiga of Komi Republic

    Directory of Open Access Journals (Sweden)

    S. I. Tarasov

    2018-02-01

    Full Text Available Rock dumps of coalmines have high potential for forest regeneration and environmental capacity, which are dependent on the technology of reclamation and the properties of technogenic soils and grounds. Traditional forestry methods for obtaining the main criteria of biological indicators of woody vegetation were used in the study as follows: ground seed germination, seedling planting technology, composition and increment of tree stands, root structure, care harvesting of undergrowth, biotopic classification. Natural overgrowing of dumps is dependent on the availability of seeds and conditions for their germination and subsequent growth. Most of the zonal tree and shrub species are able to colonize and grow on the coalmine dumps. Mineralization of the dumps surfaces without rich soil stratum, porosity of the upper horizon of lithostratum, and low nutrient content (nitrogen give benefits in the growth and subsequent formation of birch, pine and sea-buckthorn stands. Afforestation is the cheapest and most effective method of biological reclamation. The analysis of artificial reforestation shows the probability of targeted plantation cultivation of various tree species. The use of a wide range of tree and shrub species make it possible to create biologically diverse intrazonal technogenic ecosystems with high recreational and economic productivity. Wildfires spreading out in spring season on herbaceous rags limit the overgrowth of the dumps by forest vegetation. Two-year cyclical increment decline of trees due to provocative spring warming takes place. The zoogenic factor, especially zoo chores distribution of berry plants, has essential value for forest forming process. By the results of forest formation analysis at rock dumps, alveolate-hilly technology of mine reclamation was developed, which allows to significantly improve dumps’ afforestation capacity, their biological posttechnogenic diversity and productivity.

  4. Specifics of stands formation at coalmine dumps in forest-steppe zone

    Directory of Open Access Journals (Sweden)

    R. T. Murzakmаtov

    2018-02-01

    Full Text Available Rock dumps of coalmines have high potential for forest regeneration and environmental capacity, which are dependent on the technology of reclamation and the properties of technogenic soils and grounds. Traditional forestry methods for obtaining the main criteria of biological indicators of woody vegetation were used in the study as follows: ground seed germination, seedling planting technology, composition and increment of tree stands, root structure, care harvesting of undergrowth, biotopic classification. Natural overgrowing of dumps is dependent on the availability of seeds and conditions for their germination and subsequent growth. Most of the zonal tree and shrub species are able to colonize and grow on the coalmine dumps. Mineralization of the dumps surfaces without rich soil stratum, porosity of the upper horizon of lithostratum, and low nutrient content (nitrogen give benefits in the growth and subsequent formation of birch, pine and sea-buckthorn stands. Afforestation is the cheapest and most effective method of biological reclamation. The analysis of artificial reforestation shows the probability of targeted plantation cultivation of various tree species. The use of a wide range of tree and shrub species make it possible to create biologically diverse intrazonal technogenic ecosystems with high recreational and economic productivity. Wildfires spreading out in spring season on herbaceous rags limit the overgrowth of the dumps by forest vegetation. Two-year cyclical increment decline of trees due to provocative spring warming takes place. The zoogenic factor, especially zoo chores distribution of berry plants, has essential value for forest forming process. By the results of forest formation analysis at rock dumps, alveolate-hilly technology of mine reclamation was developed, which allows to significantly improve dumps’ afforestation capacity, their biological posttechnogenic diversity and productivity.

  5. The plants with essence oil are potential radio protectors

    International Nuclear Information System (INIS)

    Rzayev, N.R.; Guluyev, N.T.

    2010-01-01

    result of burn of fire brigade servants and etc. from fat oils, extractions and carotineoids of basil, tarragon and melissa will be continued. As there are much unsaturated double related compounds in the content of basil, melissa and tarragon crops like carotenoids, flavanoids and antosians it is possible to produce medicaments and cremes with protector properties against radioactive irradiations. Because of antioxidant property substances possession in the content of these crops' extractions their application ways in meat and fish canning production of industrial significance will be investigated in future. It is possible to product toothpastes (anticaries diseases) from the oil and extractions obtained of them together with Sea-Buckthorn oil. Constant hybrid basil melissa lines are available to use in food industry as green vegetable.

  6. A systematic review of anti-obesity medicinal plants - an update.

    Science.gov (United States)

    Hasani-Ranjbar, Shirin; Jouyandeh, Zahra; Abdollahi, Mohammad

    2013-06-19

    Obesity is the most prevalent health problem affecting all age groups, and leads to many complications in the form of chronic heart disease, diabetes mellitus Type 2 and stroke. A systematic review about safety and efficacy of herbal medicines in the management of obesity in human was carried out by searching bibliographic data bases such as, PubMed, Scopus, Google Scholar, Web of Science, and IranMedex, for studies reported between 30th December 2008 to 23rd April 2012 on human or animals, investigating the beneficial and harmful effects of herbal medicine to treat obesity. Actually we limited our search to such a narrow window of time in order to update our article published before December of 2008. In this update, the search terms were "obesity" and ("herbal medicine" or "plant", "plant medicinal" or "medicine traditional") without narrowing or limiting search items. Publications with available abstracts were reviewed only. Total publications found in the initial search were 651. Total number of publications for review study was 33 by excluding publications related to animals study.Studies with Nigella Sativa, Camellia Sinensis, Crocus Sativus L, Seaweed laminaria Digitata, Xantigen, virgin olive oil, Catechin enriched green tea, Monoselect Camellia, Oolong tea, Yacon syrup, Irvingia Gabonensi, Weighlevel, RCM-104 compound of Camellia Sinensis, Pistachio, Psyllium fibre, black Chinese tea, sea buckthorn and bilberries show significant decreases in body weight. Only, alginate-based brown seaweed and Laminaria Digitata caused an abdominal bloating and upper respiratory tract infection as the side effect in the trial group. No other significant adverse effects were reported in all 33 trials included in this article.In conclusion, Nigella Sativa, Camellia Synensis, Green Tea, and Black Chinese Tea seem to have satisfactory anti-obesity effects. The effect size of these medicinal plants is a critical point that should be considered for interpretation. Although there

  7. INVESTIGATION OF THE PROPERTIES OF MARMALADE WITH PLANT CRYOADDITIVES DURING STORAGE

    Directory of Open Access Journals (Sweden)

    N. Shmatchenko

    2018-04-01

    Full Text Available This article presents the results of studies of the properties of marmalade with natural plant cryoadditives during storage for 3 months (90 days. To improve the organoleptic characteristics and antioxidant properties of marmalade, plant additives of cryogenic origin were used, namely cryopastes from quince, apple, carrot, pumpkin, grapes, and cryopowders from rose hips, sea buckthorn, and grapes. It is shown that the use of plant cryoadditives makes it possible to increase the biological value and antioxidant properties of new types of marmalade. For new types of marmalade with cryoadditives, during its shelf life, these properties have not been studied earlier. However, it should be noted that such studies are necessary and relevant for solving the problem of preserving the quality indicators and antioxidant capacity of marmalade with cryoadditives. New kinds of marmalade have high organoleptic characteristics during the whole storage period. It is shown that the storage conditions of marmalade, according to current regulatory documentation, are suitable for new marmalade products, since the physico-chemical and microbiological indicators vary, but remain within acceptable limits. Thus, the moisture content decreases to almost 31%, the acidity rises by 12–16%, compared to freshly prepared samples. The content of reducing substances increases by 30–64%. It is determined that the value of the antioxidant capacity of all the samples developed is much higher – almost 2.3–8 times than the same figure for the control sample. After the storage period, the antioxidant capacity is reduced by 15–35%, but this data also significantly exceeds the antioxidant capacity of the control sample. Storage of marmalade with cryopastes and cryopowders for 90 days is possible, since all the basic physico-chemical, microbiological, and organoleptic indicators at the end of shelf life meet the requirements. Indicators of antioxidant capacity for them also

  8. The development of polymeric pellicles with gentamicine sulfate for therapeutic correction of cervical erosion (pseudoerosion

    Directory of Open Access Journals (Sweden)

    T. M. Litvinenko

    2014-08-01

    Full Text Available Introduction.Cervical erosionsoccur in 12-15%gynaecological diseases. Erosion is a damage of epithelialmucous membrane or skin. Therapy of patients with cervical erosionsis based on selection of pathogeneticsubstantiative method of treatment. Bathes and irrigations with 20% protargol, alum, carbolic acid, potassium permanganate are used. But some authors admit the destructive influence of these procedures. Using of tampons with 10% sintomycine emulsion, cod liver oil, sea-buckthorn oil,kalanchoe sap, propolis, vagotil, cigerol, galantamine also doesn’t give desirable result. Recently polymeric pellicleswith antibacterial substances are widely used. The most perspective in this route are aminoglycoside antibiotics. That is why we chose gentamicine sulfate (broad-spectrum aminoglycoside antibiotic. The aim of study is the development of the optimal composition of vaginal pellicleswith gentamicine sulfate for gynaecological practice, scientifically substantiation of excipients: polymeric base and plasticizer. Results and discussion.Polymeric bases and plasticizers influence on gentamicine sulfate releasing from polimericpellicleshas been studied. Research on choice of optimal composition has been carried out by two-factor experiment plan. The next bases and plasticizers have been used: methylcellulose, sodium carboxymethylcellulose, soluble biopolymer, gelatin; glycerine, propylenglycol, polyethylene glycol, twin 80. Gentamicine sulfate content was 80 mg in one pellicle. Gentamicine sulfate releasing from polimericpellicleshas been investigated by the Kruvchinsky method, concentration of active substance has been detected after 45 min. As a result it has been established that base makes essential influence on the gentamicine sulfate releasing (Fexp.52,88>Ftabl. 3,9. The best plasticizer is glycerin and the most optimal base is gelatin. So the optimal composition for vaginal films has been chosen: Gentamicine sulfate0,08 g Glycerin0,7 g Gelatin0

  9. SORPTION OF CO2-EXTRACTS OF SPICES TO THE TOMATO PASTE

    Directory of Open Access Journals (Sweden)

    A. M. Gadzhieva

    2014-01-01

    Full Text Available The possibility of the production of products without the preservatives, the fillers, the chemical, undesirable aromatic substances and other additives is one of the prime advantages of technologies, based on the use of СO2- extraction. There is great theoretical and practical interest in the study of the absorption of СO2- extracts on the tomato carriers with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. The miscella of the extracts of spices is brought to the tomato paste. This was reached by the selection of СO2- miscella from the extractor under the pressure to 6 MPa. Then solvent due to a pressure decrease in the apparatus to the atmospheric instantly was moved away from the obtained material. The degrees of the absorption of the СO2- extracts of anise, ginger, amaranth and sea buckthorn are determined. Calculations were performed through the program of that composed in medium of the mathematical packet “Of mathCAD 14”. As a result conducted investigations are obtained the equations of regression for absorbing of anise, amaranth and ginger on the tomato paste depending on the concentration of individual extracts in the miscella. The possibilities of the method of complete factor experiment are shown. In the utilized method of the mathematical planning of experiment adapt the dimensionless coded composition factors x1 and x2. In the experiment is realized plan 23. Was used the central composition plan, which corresponds to the requirement of rotatable, i.e., the plan, which makes it possible to obtain the model, capable of predicting the value of the parameter of optimization with the

  10. Technology of development phytogenetic protectors and creation of raw material base

    International Nuclear Information System (INIS)

    Rzayev, N.R.; Guluyev, N.T.

    2010-01-01

    Recently in order to protect organisms from the of radiation effect radio protectors have been applied principally concerning to sulfur compounds, aminothioles and indolilalkins. Although the achievements obtained in the direction of synthetic medicines discovery, they can not completely replace the medicinal preparation of natural origin. That is why the study of natural biological active substances with radio protector property are of special importance. One of the goals of the project is the detection of plant species rich with anti mutagen and radio protector, the study of their cultivation technology, and the produce of natural raw material with high quality in planting condition. Flora of Azerbaijan is considered one of the richest countries for its species' richness. There are plant varieties for producing radio protector substances (essence-oil, lipid, lipoid, phenol compounds, pigment substances, proteins, vitamins and various amino acids). It was cultivated new varieties and forms of sea buckthorn, black caraway, basil, tarragons which are richer with their radio protector substances among these plants. In the article it is intended to prepare aerosol, consisted of essence oils and lipid mixtures, for respiratory system treatment in extreme conditions having radiation threat. The plants with essence oil are potential radio protectors. On industry scale the researches will be conducted in the direction of this technology realization. There are also some achievements in the field of working out of production technology of food products, with polyvitamins, from plants for extreme conditions and it will be improved in the process of project activies. Scientific importance of alcoholic and less alcoholic drinks in liquidating the impact of radioactive substances in organism and their disposal out of the organism have been found in literatures. It is also intended to conduct research works on production technology of medicinal preparations, liquor, alcohol of

  11. Phytotherapy of Acute Respiratory Viral Diseases

    Directory of Open Access Journals (Sweden)

    I.B. Ershova

    2016-11-01

    of acute respiratory viral infections the medicinal plants of several pharmacological groups mainly used are: plants with a tonic effect (plants containing vitamins and minerals — rose hips, black currant, sea buckthorn, citrus fruits, adaptogens and immune modulators should be used in pediatric practice with caution (Ginseng, Rhodiola Rosea, Aralia Manchurian, plants containing biogenic stimulators — the aloe latex, kalanchoe, plant with bactericidal and bacteriostatic effect (sage leaves drug, eucalyptus switchgrass, plants with anti-inflammatory effect, containing tannins — oak bark, Potentilla rhizome, rhizome of Polygonum snake, burnet, alder cones, etc; plants containing essential oils, azulene derivatives — Chamomile flowers; plants containing mucus — Farfara leaves, Plantain; Linden flowers; plants with antipyretic and diaphoretic action — plants containing vitamins (fruits of raspberry, black currant fruit, cranberry; plants containing salicylic acid derivatives — Viola tricolor, raspberry fruit; plants with protective, emollient, expectorant action containing mucus — Farfara leaves; Linden flowers, plantain leaves are large; containing alkaloids, with antitussive effect —codeine phosphate; glaucine hydrochloride grass of Thermopsis lancet; bronchodilators plants; plants with anti-allergic effect, containing flavonoids — herb of Viola tricolor, the roots of Licorice. The article presents the re­commendation of professor O.D. Barnaulov, a pediatric phytotherapist O.A. Daniluk on herbal medicine using in children, the rules of the certain medicinal plants use by Y.I. Korshikova, dosage regimen for herbal remedies for children, proposed by N.P. Menshikova, as well as practical advice on drafting phytospecies for the treatment and prophylaxis of acute respiratory viral infections and prescribing proposed by phytotherapist O.V. Kostareva.