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Sample records for buckthorn hippophae rhamnoides

  1. Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.).

    Science.gov (United States)

    Suryakumar, Geetha; Gupta, Asheesh

    2011-11-18

    ETHNOPHARMACOLOGICAL CONTEXT: This review explores the medicinal and therapeutic applications of Sea buckthorn (Hippophae rhamnoides L.) in curtailing different types of acute as well as chronic maladies. The plant is being used in different parts of the world for its nutritional and medicinal properties. Sea buckthorn based preparations have been extensively exploited in folklore treatment of slow digestion, stomach malfunctioning, cardiovascular problems, liver injury, tendon and ligament injuries, skin diseases and ulcers. In the recent years, medicinal and pharmacological activities of Sea buckthorn have been well investigated using various in vitro and in vivo models as well as limited clinical trials. Sea buckthorn has been scientifically analyzed and many of its traditional uses have been established using several biochemical and pharmacological studies. Various pharmacological activities such as cytoprotective, anti-stress, immunomodulatory, hepatoprotective, radioprotective, anti-atherogenic, anti-tumor, anti-microbial and tissue regeneration have been reported. It is clear that Sea buckthorn is an important plant because of its immense medicinal and therapeutic potential. However, several knowledge gaps identified in this paper would give impetus to new academic and R&D activities especially for the development of Sea buckthorn based herbal medicine and nutraceuticals. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  2. Remedial Prospective of Hippophae rhamnoides Linn. (Sea Buckthorn).

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    Patel, Chirag A; Divakar, Kalyani; Santani, Devdas; Solanki, Himanshu K; Thakkar, Jalaram H

    2012-01-01

    Sea buckthorn (Hippophae rhamnoides L.) constitutes thorny nitrogen fixing deciduous shrub. Sea buckthorn(SBT) is primarily valued for its very rich vitamins A, B(1), B(12), C, E, K, and P; flavonoids, lycopene, carotenoids, and phytosterols. and therapeutically important since it is rich with potent antioxidants. Scientifically evaluated pharmacological actions of SBT are like inflammation inhibited by reduced permeability, loss of follicular aggregation of lymphocytes from the inflamed synovium and suppress lymphocyte proliferation. SBT-reduced recurrence of angina, ischemic electrocardiogram which might be due to decreased myocardial oxygen consumption and inhibition of platelet aggregation induced by collagen. SBT can kill both cancer cells of S180, P388, SGC7901 and lymphatic leukemia (L1200). The antiulcer activity may be related to reduce gastric empty time, inhibiting proteolytic activity and promoting wound reparation processes of mucosa. SBT exerts antihypertensive effect in part by blocking angiotensin-2 receptor on cell surface. SBT decreased the level of stress hormones and enhanced hypoxic tolerance in animals indicating its anti-stress, adaptogenic activity. A lot of research work is still needed to find cellular and molecular mechanisms of these activities and also yet to be explored for its activity in osteoporosis, hemorrhage, cataract, urinary stone, acne, psoriasis, polyneuritis, cheilosis, glossities, baldness, anti-obesity, gout, and chronic prostitis.

  3. Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties

    NARCIS (Netherlands)

    Pop, R.M.; Weesepoel, Y.J.A.; Socaciu, C.; Pintea, A.; Vincken, J.P.; Gruppen, H.

    2014-01-01

    Berries and leaves from six varieties of Carpathians’ sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) were analysed for their carotenoid composition (free and esterified) using a combination of HPLC-PAD, GC–MS and UHPLC–PAD–ESI-MS techniques. GC–MS techniques revealed the fatty acid profile

  4. PREPARATION OF PROTOPLASTS OF SEA BUCKTHORN (HIPPOPHAE RHAMNOIDES L.

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    M. V. Skaptsov

    2015-04-01

    Full Text Available The aim of research was to study the effect of a method of protoplast isolation on Hippophae rhamnoides cell viability. The main objectives were the release of protoplasts H. rhamnoides from callus tissue by a combination of mechanical and enzymatic effects, as well as evaluation of the general state of isolated cells after isolation and purification. The objects of studies were mesophyll cells and leaf mesophyll explants introduced in culture in vitro. It is assumed that considerable damage of cells in the process of mechanical-enzymatic treatment is not only a result of mechanical impact, but also an effect of the release of metabolic products (polyphenolic components of cells and their decay. Also protoplasts are dedifferentiated and simplify the process of differentiation in various breeding and biotechnological experiments. As a result of the work, an optimized method of H. rhamnoides protoplast isolation with decreased levels of cell damage is set up. It is revealed that important factors for high yield of viable protoplasts are: proper concentration of D-mannite as an osmoticum; proper concentration of cellulase R10 and macerozime R10; use of sodium tiosulfate as an antioxidante; proper time of incubation of leaf explants in medium with enzymes.

  5. Report - Antibacterial activity of sea buckthorn (Hippophae rhamnoides L.) against methicillin resistant Staphylococcus aureus (MRSA).

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    Qadir, Muhammad Imran; Abbas, Khizar; Younus, Adnan; Shaikh, Rehan Sadiq

    2016-09-01

    Objective of the present study was to investigate the antibacterial activity of Sea buckthorn (Hippophae rhamnoides L.) berries and leaves against methicillin resistant Staphylococcus aureus (MRSA) by using the standard disc diffusion method. Chloroform, n-hexane and aqueous extract of the plant parts were used. Doses of 2mg/ml, 4 mg/ml and 6mg/ml were tested against the microorganism, and the zone of inhibition was compared against the standard drug vancomycin. Results indicated that n-hexane and chloroform extracts of berries and n-hexane extract leaves showed significant (p<0.05) antibacterial activity comparable with vancomycin. It was concluded from the study that extracts berries and leaves of Hippophae rhamnoides have antibacterial activity against MRSA.

  6. Comparative assessment of phytochemical profiles, antioxidant and antiproliferative activities of Sea buckthorn (Hippophaë rhamnoides L.) berries.

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    Guo, Ruixue; Guo, Xinbo; Li, Tong; Fu, Xiong; Liu, Rui Hai

    2017-04-15

    Phytochemical profiles, antioxidant and antiproliferative activities of berry extracts were evaluated and compared in four subspecies of Sea buckthorn (Hippophaë rhamnoides L.). Among the subspecies, Hippophaë rhamnoides L. subsp. sinensis exhibited highest total phenolics content (38.7±1.3mgGA equiv./g DW) and corresponding total antioxidant activity. Whereas maximum cellular antioxidant and antiproliferative activities were determined in Hippophaë rhamnoides L. subsp. yunnanensis. Total antioxidant activity was significantly associated to total phenolics, isorhamnetin-3-rutinoside and isorhamnetin-3-glucoside. The cellular antioxidant activity and antiproliferative activity of phytochemicals were fairly correlated to phenolic acids and flavonoid aglycones. Lower median effective dose (EC 50 ) of individual compounds against human liver cancer HepG2 cells proliferation studies confirmed the better correlation between antiproliferative activity of Sea buckthorn extracts and flavonoid aglycones, including isorhamnetin, quercetin and kaempferol. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Biotic and abiotic soil factors in the succession of sea buckthorn, Hippophae rhamnoides L. in coastal sand dunes

    NARCIS (Netherlands)

    Zoon, F.

    1995-01-01

    Decline and succession in dune scrub vegetation of sea buckthorn, Hippophaë rhamnoides L. in the Netherlands was previously found to be stimulated by biotic soil factors. In the present study, the role of plant-parasitic nematodes and root fungi was investigated. A

  8. Protein and Amino Acid Composition of Sea Buckthorn Seeds (Hippophae rhamnoides mongolica Rouse

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    M. Uransanaa

    2003-06-01

    Full Text Available The wild sea buckthorn (Hippophae rhamnoides L is distributed in Mongeiia along the basin of rivers and lakes, which disposed between high mountains in western and northern parts ofthe country. The wild sea buckthorn is a cold tolerant unique plant with beneficial value of medicinal and cosmetic products. About 7.2% of wild sea buckthorn berries belongs to seeds and the pratein in seeds composed 37.79%, while the protein in shells equal to 15.25%. Best extraction of protiens was successful at value of pH=l- 3 or pH=10-12. The soluble protein content ofthe whole proteins in seeds was 37.4% at the value of pH=1 and 63.85% at pH=12, while the shell proteins were 16.2% and 22.8%, respectively. The tan1 content of essential amino acids is composed about 43.32-45.04% of whole protein. ~her~f~ie, it can be conclude that the sea buckthorn seeds are valuable resourse containing respectable amount of valuable proteins. Qn the other hand, seeds are undamageble during technological procedure and, therefore further precessing as raw material is advisable.

  9. Fatty Acid Composition of Developing Sea Buckthorn (Hippophae rhamnoides L.) Berry and the Transcriptome of the Mature Seed

    OpenAIRE

    Fatima, Tahira; Snyder, Crystal L.; Schroeder, William R.; Cram, Dustin; Datla, Raju; Wishart, David; Weselake, Randall J.; Krishna, Priti

    2012-01-01

    BACKGROUND: Sea buckthorn (Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18:2 ω-6) and α-linolenic (18:3 ω-3) acids, and pulp oil that contains high levels of monounsaturated palmitoleic acid (16:1 ω-7). Sea buckthorn is fast gaining popularity as a source of functional food and nutr...

  10. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

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    Sajfrtová, Marie; Ličková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk

    2010-01-01

    Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40–80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide. PMID:20480045

  11. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L. Seeds

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    Marie Sajfrtová

    2010-04-01

    Full Text Available Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L. seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40-80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.

  12. Sea Buckthorn (Hippophae rhamnoidesL.) Leaf Extracts Protect Neuronal PC-12 Cells from Oxidative Stress.

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    Cho, Chi Heung; Jang, Holim; Lee, Migi; Kang, Hee; Heo, Ho Jjn; Kim, Dae-Ok

    2017-07-28

    The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn ( Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversed-phase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.

  13. Frost hardiness of introduced sea buckthorn (Hippophae rhamnoides L. genotypes in Central Russia

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    Ozherelieva Zoya

    2016-04-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides L. genotypes of different ecological and geographical origins were tested. Frost hardiness extent was determined during winter by artificial freezing, which allowed identify varietal differences in frost hardiness of buds and tissues of sea buckthorn shoots. In order to identify differences in adaptation to low temperature we determined the fractional composition of water in annual shoots; and proline and malone dialdehyde concentration and total amylase activity in bark of shoots of female and male genotypes. In December, the sea buckthorn genotypes were in deep dormancy, when maximum frost hardiness of buds and tissues of annual shoots occurred. During the next months, the frost hardiness of buds and shoot tissues decreased during the state of exogenous dormancy. The performed experiment gave an opportunity to select hardy genotypes ‘Kenigsbergskaya’, ‘Surpriz Baltiki’, B 23-34, and ‘Prima Dona’ for use in further breeding, as they had demonstrated stable hardiness during the whole winter period according to all of the components of winter hardiness.

  14. Sea Buckthorn (Hippophaë rhamnoides ssp. rhamnoides) Berries in Nordic Environment: Compositional Response to Latitude and Weather Conditions.

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    Zheng, Jie; Kallio, Heikki; Yang, Baoru

    2016-06-22

    Flavonol glycosides (FGs) in sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) berries of varieties 'Tytti' and 'Terhi', cultivated in northern Finland (68°02' N) for six years and southern Finland (60°23' N) for seven years, were investigated and compared by HPLC-DAD-ESI-MS/MS. The average total content of 23 identified glycosides of isorhamnetin and quercetin was 103 ± 23 and 110 ± 21 mg/100 g fresh berries in 'Terhi' and 'Tytti', respectively. The total contents of FGs, flavonol diglycosides, and triglycosides in both varieties were higher in the north than in the south, whereas total flavonol monoglycoside content behaved vice versa (p < 0.05). Among the 89 weather variables studied, the sum of the daily mean temperatures that are 5 °C or higher from the start of growth season until the day of harvest was the most important variable which associated negatively with the accumulation of FGs in berries. Such influence was much stronger in berries from the north than from the south.

  15. Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties.

    Science.gov (United States)

    Pop, Raluca Maria; Weesepoel, Yannick; Socaciu, Carmen; Pintea, Adela; Vincken, Jean-Paul; Gruppen, Harry

    2014-03-15

    Berries and leaves from six varieties of Carpathians' sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) were analysed for their carotenoid composition (free and esterified) using a combination of HPLC-PAD, GC-MS and UHPLC-PAD-ESI-MS techniques. GC-MS techniques revealed the fatty acid profile specific for each berry variety, while targeted UHPLC-MS analysis identified the fatty acids involved in carotenoids esterification: palmitic (C16:0), myristic (C14:0) and stearic (C18:0). Total carotenoid content varied between 53 and 97 mg/100g dry weight in berries, and between 3.5 and 4.2mg/100g DW in leaves. The carotenoid di-esters represented the main fraction among berry varieties having zeaxanthin di-palmitate as major compound, while leaves contained only free carotenoids like lutein, β-carotene, violaxanthin and neoxanthin. Principal component analysis identified the suitable carotenoid biomarkers characteristic for the Carpathians' sea buckthorn from Romania with contribution to their taxonomic classification and authenticity recognition. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Sea buckthorn (Hippophae rhamnoides L.) vegetative parts as an unconventional source of lipophilic antioxidants.

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    Górnaś, Paweł; Šnē, Elga; Siger, Aleksander; Segliņa, Dalija

    2016-07-01

    The profile of lipophilic antioxidants in different vegetative parts (leaves, shoots, buds and berries) was studied in sea buckthorn (Hippophae rhamnoides L.) male and female plants collected in the end of spring. Five lipophilic compounds, i.e. three tocopherol homologues (α, β and γ), plastochromanol-8 and β-carotene, were identified in each vegetative part of male and female sea buckthorn plants at the following concentrations: 7.25-35.41, 0.21-2.43, 0.41-1.51, 0.19-1.79 and 4.43-24.57 mg/100 g dry weight basis. Additionally, significant amounts of α-tocotrienol (1.99 mg/100 g dry weight basis) were detected in buds. The α-tocopherol and β-carotene were predominant lipophilic antioxidants in each vegetative part, accounting for 78.3-97.0% of identified compounds. The greatest amounts of lipophilic antioxidants were found in leaves, especially of female plants. Nevertheless, apart from leaves, also shoots of plants of both sexes seem to be a good source of α-tocopherol and β-carotene.

  17. Sea buckthorn (Hippophae rhamnoides L. vegetative parts as an unconventional source of lipophilic antioxidants

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    Paweł Górnaś

    2016-07-01

    Full Text Available The profile of lipophilic antioxidants in different vegetative parts (leaves, shoots, buds and berries was studied in sea buckthorn (Hippophae rhamnoides L. male and female plants collected in the end of spring. Five lipophilic compounds, i.e. three tocopherol homologues (α, β and γ, plastochromanol-8 and β-carotene, were identified in each vegetative part of male and female sea buckthorn plants at the following concentrations: 7.25–35.41, 0.21–2.43, 0.41–1.51, 0.19–1.79 and 4.43–24.57 mg/100 g dry weight basis. Additionally, significant amounts of α-tocotrienol (1.99 mg/100 g dry weight basis were detected in buds. The α-tocopherol and β-carotene were predominant lipophilic antioxidants in each vegetative part, accounting for 78.3–97.0% of identified compounds. The greatest amounts of lipophilic antioxidants were found in leaves, especially of female plants. Nevertheless, apart from leaves, also shoots of plants of both sexes seem to be a good source of α-tocopherol and β-carotene.

  18. Bioactive profile, health benefits and safety evaluation of sea buckthorn (Hippophae rhamnoides L.: A review

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    Touseef Ahmed Wani

    2016-12-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides L. contains a large number of versatile compounds with antioxidant and hence medical properties that have been reported from time to time. Intensive work on the medical properties of sea buckthorn has concluded incredible results like the effects on atherosclerosis, anti-visceral obesity, platelet aggregation, inflammation, adverse stressful situations, and that of liver injury. Only a few studies have been conducted on the safety evaluation of the plant extract but importantly no study has deemed it unsafe for animal or human consumption till date. Considering the fact that no significant changes have been observed in organ weight/body weight ratios, of any vital organ studied (except liver and kidney in 1 g/kg and 2 g/kg body weight doses, respectively and biochemical and hematological parameters in different animal trials with an lethal dose for 50% reduction of population (LD50 of >10 g/kg when given orally, there is scope for further investigations regarding its safety in the daily diet as a protective medicine.

  19. Effects on Quality Properties of Pork Sausages Enriched with Sea Buckthorn (Hippophae rhamnoides L.

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    Anna Marietta Salejda

    2017-01-01

    Full Text Available The present study was aimed at evaluating the effect of a sea buckthorn (Hippophae rhamnoides L. fruit extract on selected quality properties of cooked sausages. The ethanolic extract of sea buckthorn fruit (SBE incorporated at the highest level (3% significantly affected the pH, weight losses, and instrumental color parameters of sausages. The SBE deteriorated organoleptic properties of sausages like juiciness, overall appearance, texture, and taste; however the sausages manufactured with 1.5% SBE were scored higher for color and almost the same as control for smell acceptance. Textural parameters like hardness, springiness, gumminess, and chewiness of cooked sausages decreased along with SBE addition. After 28 days of storage, the samples with 1.5% SBE addition were as springy, hard, and gummy as the control ones. Incorporation of SBE increased the shelf life of sausages. The highest inhibition of lipid oxidation was observed in the samples manufactured with 1.5% SBE. The SBE significantly improved the microbial qualities of sausages.

  20. Hippophae rhamnoides

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    Sougrakpam, Yaiphabi; Deswal, Renu

    2016-10-01

    Hippophae rhamnoides is a hardy shrub capable of growing under extreme environmental conditions namely, high salt, drought and cold. Its ability to grow under extreme conditions and its wide application in pharmaceutical and nutraceutical industry calls for its in-depth analysis. N-glycoproteome mining by con A affinity chromatography from seedling was attempted. The glycoproteome was resolved on first and second dimension gel electrophoresis. A total of 48 spots were detected and 10 non-redundant proteins were identified by MALDI-TOF/TOF. Arabidopsis thaliana protein disulfide isomerase-like 1-4 (ATPDIL1-4) electron transporter, protein disulphide isomerase, calreticulin 1 (CRT1), glycosyl hydrolase family 38 (GH 38) protein, phantastica, maturase k, Arabidopsis trithorax related protein 6 (ATXR 6), cysteine protease inhibitor were identified out of which ATXR 6, phantastica and putative ATPDIL1-4 electron transporter are novel glycoproteins. Calcium binding protein CRT1 was validated for its calcium binding by stains all staining. GO analysis showed involvement of GH 38 and ATXR 6 in glycan and lysine degradation pathways. This is to our knowledge the first report of glycoproteome analysis for any Elaeagnaceae member.

  1. Role of Flavonols and Proanthocyanidins in the Sensory Quality of Sea Buckthorn (Hippophaë rhamnoides L.) Berries.

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    Ma, Xueying; Yang, Wei; Laaksonen, Oskar; Nylander, Merja; Kallio, Heikki; Yang, Baoru

    2017-11-15

    Sensory profile, flavonols, proanthocyanidins, sugars, and organic acids were investigated in purees of six sea buckthorn (Hippophaë rhamnoides) cultivars. The sensory profiles of the purees were dominated by intense sourness followed by astringency and bitterness due to the high content of malic acid. Malic acid and isorhamnetin glycosides, especially isorhamnetin-3-O-sophoroside-7-O-rhamnoside, had close association with the astringent attributes in the different purees, whereas some of the known astringent compounds such as proanthocyanidin dimers and trimers or quercetin glycosides, had less impact. Moreover, the ratios between contents of acids and phenolic compounds were more important predictors of bitterness than the individual variables alone. Astringency and bitterness are important sensory factors for the consumer acceptance of sea buckthorn products. The current study provides new knowledge on the correlations between sensory properties and composition and supports industrial utilization of the sea buckthorn berries.

  2. Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn (Hippophaë rhamnoides L.) Berries.

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    Teleszko, Mirosława; Wojdyło, Aneta; Rudzińska, Magdalena; Oszmiański, Jan; Golis, Tomasz

    2015-04-29

    The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophaë rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p < 0.05).

  3. Identification, quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn (Hippophae rhamnoides ssp. sinensis).

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    Chen, Chu; Xu, Xue-Min; Chen, Yang; Yu, Meng-Yao; Wen, Fei-Yan; Zhang, Hao

    2013-12-01

    A novel acylated flavonol glycoside: isorhamnetin (3-O-[(6-O-E-sinapoyl)-β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside) (1), together with two known acylated flavonol glycosides: quercetin (3-O-[(6-O-E-sinapoyl)-β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside) (2) and kaempferol (3-O-[(6-O-E-sinapoyl)-β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside) (3) were isolated from the n-butanol fraction of sea buckthorn (Hippophae rhamnoides ssp. sinensis) berries for the first time by chromatographic methods, and their structures were elucidated using UV, MS, (1)H and (13)C NMR, and 2D NMR. Compounds 1-3 showed good scavenging activities, with respective IC50 values of 8.91, 4.26 and 30.90 μM toward the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical; respective Trolox equivalent antioxidant capacities of 2.89, 4.04 and 2.44 μM μM(-1) toward 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonate (ABTS) radical. The quantitative analysis of the isolated acylated flavonol glycosides was performed by HPLC-DAD method. The contents of compounds 1-3 were in the range of 12.2-31.4, 4.0-25.3, 7.5-59.7 mg/100 g dried berries and 9.1-34.5, 75.1-182.1, 29.2-113.4 mg/100 g dried leaves, respectively. Copyright © 2013. Published by Elsevier Ltd.

  4. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L. Seed against Visible Light-Induced Retinal Degeneration in Vivo

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    Yong Wang

    2016-05-01

    Full Text Available Dietary proanthocyanidins (PACs as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L. seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6 and angiogenesis (VEGF levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  5. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L.) Seed against Visible Light-Induced Retinal Degeneration in Vivo.

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    Wang, Yong; Zhao, Liang; Huo, Yazhen; Zhou, Feng; Wu, Wei; Lu, Feng; Yang, Xue; Guo, Xiaoxuan; Chen, Peng; Deng, Qianchun; Ji, Baoping

    2016-05-02

    Dietary proanthocyanidins (PACs) as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L.) seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day) for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6) and angiogenesis (VEGF) levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  6. Nutritional assessment of processing effects on major and trace element content in sea buckthorn juice (Hippophaë rhamnoides L. ssp. rhamnoides).

    Science.gov (United States)

    Gutzeit, D; Winterhalter, P; Jerz, G

    2008-08-01

    Processing effects on the mineral content were investigated during juice production from sea buckthorn (Hippophaë rhamnoides L. ssp. rhamnoides, Elaeagnaceae) using berries from 2 different growing areas. The major and trace elements of sea buckthorn berries and juices were determined by atomic absorption spectroscopy (AAS)--(calcium, iron, magnesium, potassium, sodium) and inductively coupled plasma-mass spectrometry (ICP-MS)--(arsenic, boron, chromium, copper, manganese, molybdenum, nickel, selenium, zinc). Potassium is the most abundant major element in sea buckthorn berries and juices. The production process increased the potassium content in the juice by about 20%. Moreover, the processing of juice increased the value of manganese up to 32% compared to the content in berries. During industrial juice production, the technological steps caused a loss of about 53% to 77% of the chromium concentration, 50% of the copper content, 64% to 75% of the molybdenum amount, and up to 45% of the iron concentration in the final juice product. Consumption of sea buckthorn juice represents a beneficial source of chromium, copper, manganese, molybdenum, iron, and potassium for the achievement of the respective dietary requirements.

  7. Data on coal dumps retrieving in petroşani basin using sea buckthorn (Hippophae rhamnoides

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    Andreea BRASOVAN

    2008-05-01

    Full Text Available This contribution presents data on the sea buckthorn (Hippöphae rhamnoides, with focus on its value on coal dumps retrieving in the Petroşani Basin. Due to its peculiar roots exposing a lot of nodules, this plant has an important role in fixing the atmospheric azoth and soil genesis. It is very adaptable to any kind of soil and has wide climatic exigencies, but is depending on light intensity. It has a large dissemination capacity, due to its light and numerous seeds. Besides its utilization on dump retrieving, sea buckthorn is used in medicine and pharmacy, as well as in organic fuel production.

  8. Sea buckthorn (Hippophae rhamnoides L. as a potential source of nutraceutics and its therapeutic possibilities - a review

    Directory of Open Access Journals (Sweden)

    Jana Krejcarová

    2015-01-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides L. is in the focus of interest mainly for its positive effects on health of both human and animal organisms. The whole plant of sea buckthorn and especially its berries are a source of a large number of different bioactive compounds. The greatest attention has been drawn to its high content of vitamins, minerals, natural antioxidants, n-3 and n-6 fatty acids, and proteins. Sea buckthorn is valued for its antioxidant, cardioprotective, antiatherogenic, antidiabetic, hepatoprotective, anti-carcinogenic, immunomodulatory, antiviral, antibacterial, anti-inflammatory and vasorelaxant effects. Due to these and other positive effects, the plant is included in both human and animal nutrition, in the latter case to increase the biological value of animal products. This review summarises the botanical characteristics of sea buckthorn, lists the bio-active substances contained in individual parts of the plant, their effects in the prevention of a number of different diseases and their possible utilisation in human and animal nutrition.

  9. Energy efficiency analysis of the sea buckthorn (Hippophae rhamnoides) fruits quick freezing

    OpenAIRE

    I. A. Korotkiy; E. V. Korotkaya; KIREEV V.V.

    2016-01-01

    Low temperature preservation of perishable produce is a widespread technology of it's long-term conservation, at the same time freezing of foods being an extremely power-consuming process. When developing low temperature preservation technology it is important to aim both at retaining high quality of frozen food and improving the energy efficiency of the processes. This article presents the results of research that explores the energy efficiency when freezing the sea buckthorn ( Hippophae rha...

  10. Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L.) berry and the transcriptome of the mature seed.

    Science.gov (United States)

    Fatima, Tahira; Snyder, Crystal L; Schroeder, William R; Cram, Dustin; Datla, Raju; Wishart, David; Weselake, Randall J; Krishna, Priti

    2012-01-01

    Sea buckthorn (Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18:2 ω-6) and α-linolenic (18:3 ω-3) acids, and pulp oil that contains high levels of monounsaturated palmitoleic acid (16:1 ω-7). Sea buckthorn is fast gaining popularity as a source of functional food and nutraceuticals, but currently has few genomic resources; therefore, we explored the fatty acid composition of Canadian-grown cultivars (ssp. mongolica) and the sea buckthorn seed transcriptome using the 454 GS FLX sequencing technology. GC-MS profiling of fatty acids in seeds and pulp of berries indicated that the seed oil contained linoleic and α-linolenic acids at 33-36% and 30-36%, respectively, while the pulp oil contained palmitoleic acid at 32-42%. 454 sequencing of sea buckthorn cDNA collections from mature seeds yielded 500,392 sequence reads, which identified 89,141 putative unigenes represented by 37,482 contigs and 51,659 singletons. Functional annotation by Gene Ontology and computational prediction of metabolic pathways indicated that primary metabolism (protein>nucleic acid>carbohydrate>lipid) and fatty acid and lipid biosynthesis pathways were highly represented categories. Sea buckthorn sequences related to fatty acid biosynthesis genes in Arabidopsis were identified, and a subset of these was examined for transcript expression at four developing stages of the berry. This study provides the first comprehensive genomic resources represented by expressed sequences for sea buckthorn, and demonstrates that the seed oil of Canadian-grown sea buckthorn cultivars contains high levels of linoleic acid and α-linolenic acid in a close to 1:1 ratio, which is beneficial for human health. These data provide the foundation for further studies on sea buckthorn oil, the enzymes involved

  11. Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L. berry and the transcriptome of the mature seed.

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    Tahira Fatima

    Full Text Available BACKGROUND: Sea buckthorn (Hippophae rhamnoides L. is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18:2 ω-6 and α-linolenic (18:3 ω-3 acids, and pulp oil that contains high levels of monounsaturated palmitoleic acid (16:1 ω-7. Sea buckthorn is fast gaining popularity as a source of functional food and nutraceuticals, but currently has few genomic resources; therefore, we explored the fatty acid composition of Canadian-grown cultivars (ssp. mongolica and the sea buckthorn seed transcriptome using the 454 GS FLX sequencing technology. RESULTS: GC-MS profiling of fatty acids in seeds and pulp of berries indicated that the seed oil contained linoleic and α-linolenic acids at 33-36% and 30-36%, respectively, while the pulp oil contained palmitoleic acid at 32-42%. 454 sequencing of sea buckthorn cDNA collections from mature seeds yielded 500,392 sequence reads, which identified 89,141 putative unigenes represented by 37,482 contigs and 51,659 singletons. Functional annotation by Gene Ontology and computational prediction of metabolic pathways indicated that primary metabolism (protein>nucleic acid>carbohydrate>lipid and fatty acid and lipid biosynthesis pathways were highly represented categories. Sea buckthorn sequences related to fatty acid biosynthesis genes in Arabidopsis were identified, and a subset of these was examined for transcript expression at four developing stages of the berry. CONCLUSION: This study provides the first comprehensive genomic resources represented by expressed sequences for sea buckthorn, and demonstrates that the seed oil of Canadian-grown sea buckthorn cultivars contains high levels of linoleic acid and α-linolenic acid in a close to 1:1 ratio, which is beneficial for human health. These data provide the foundation for further

  12. Effects of total flavonoids of sea buckthorn ( Hippophae rhamnoides L.) on cytotoxicity of NK92-MI cells.

    Science.gov (United States)

    Hou, Diandong; Wang, Decheng; Ma, Xiande; Chen, Wenna; Guo, Shengnan; Guan, Hongquan

    2017-12-01

    Sea buckthorn ( Hippophae rhamnoides L.) has multifarious medicinal properties including immunoregulatory effect. The total flavonoids of Hippophae rhamnoides L. (TFH) are the main active components isolated from berries of sea buckthorn. The aim of this study was to evaluate the effects of TFH on the cytotoxicity of NK92-MI cells and its possible mechanisms. NK92-MI cells were treated with TFH (2.5 or 5.0 mg/L) or phosphate-buffered saline (PBS) for 24 h, the cytotoxicity against K562 was detected by measuring the release of lactate dehydrogenase (LDH), expression levels of NCRs (NKp30, NKp44, NKp46) and NKG2D were detected by flow cytometry, and expression levels of perforin and granzyme B were detected by western blot. Cytokine Antibody Arrays with 80 cytokine proteins were used to profile the effect of TFH on cytokines. Western blot was adopted to detect the effects of TFH on STAT1, STAT4, and STAT5 signal pathway. Compared with the normal control group, TFH could significantly enhance NK92-MI cell cytotoxicity against K562 cells, upregulate expressions of NKp44, NKp46, perforin, and granzyme B. TFH could upregulate expressions of IL-1α, IL-2, IL-7, IL-15, CSF-2, CSF-3, MCP-1, MIG, IFN-γ, TNF-α, and TNF-β and downregulate expressions of IL-16, MIP-1β, CX3CL-1, and MIF. TFH could increase expressions of phospho-STAT1 and phospho-STAT5. The results suggest that TFH stimulated NK92-MI cells to activate and enhance cytotoxicity of NK92-MI cells.

  13. Fatty acids in berry lipids of six sea buckthorn (Hippophae rhamnoides L., subspecies carpatica cultivars grown in Romania

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    Dulf Francisc V

    2012-09-01

    Full Text Available Abstract Background A systematic mapping of the phytochemical composition of different sea buckthorn (Hippophae rhamnoides L. fruit subspecies is still lacking. No data relating to the fatty acid composition of main lipid fractions from the berries of ssp. carpatica (Romania have been previously reported. Results The fatty acid composition of the total lipids (oils and the major lipid fractions (PL, polar lipids; FFA, free fatty acids; TAG, triacylglycerols and SE, sterol esters of the oils extracted from different parts of six sea buckthorn berry subspecies (ssp. carpatica cultivated in Romania were investigated using the gas chromatography-mass spectrometry (GC-MS. The dominating fatty acids in pulp/peel and whole berry oils were palmitic (23-40%, oleic (20-53% and palmitoleic (11-27%. In contrast to the pulp oils, seed oils had higher amount of polyunsaturated fatty acids (PUFAs (65-72%. The fatty acid compositions of TAGs were very close to the compositions of corresponding seed and pulp oils. The major fatty acids in PLs of berry pulp/peel oils were oleic (20-40%, palmitic (17-27%, palmitoleic (10-22% and linoleic (10%-20% acids, whereas in seeds PLs, PUFAs prevailed. Comparing with the other lipid fractions the SEs had the highest contents of saturated fatty acids (SFAs. The fatty acid profiles of the FFA fractions were relatively similar to those of TAGs. Conclusions All parts of the analyzed sea buckthorn berry cultivars (ssp. carpatica exhibited higher oil content then the other European or Asiatic sea buckthorn subspecies. Moreover, the pulp/peel oils of ssp. carpatica were found to contain high levels of oleic acid and slightly lower amounts of linoleic and α-linolenic acids. The studied cultivars of sea buckthorn from Romania have proven to be potential sources of valuable oils.

  14. Fatty acids in berry lipids of six sea buckthorn (Hippophae rhamnoides L., subspecies carpatica) cultivars grown in Romania.

    Science.gov (United States)

    Dulf, Francisc V

    2012-09-20

    A systematic mapping of the phytochemical composition of different sea buckthorn (Hippophae rhamnoides L.) fruit subspecies is still lacking. No data relating to the fatty acid composition of main lipid fractions from the berries of ssp. carpatica (Romania) have been previously reported. The fatty acid composition of the total lipids (oils) and the major lipid fractions (PL, polar lipids; FFA, free fatty acids; TAG, triacylglycerols and SE, sterol esters) of the oils extracted from different parts of six sea buckthorn berry subspecies (ssp. carpatica) cultivated in Romania were investigated using the gas chromatography-mass spectrometry (GC-MS). The dominating fatty acids in pulp/peel and whole berry oils were palmitic (23-40%), oleic (20-53%) and palmitoleic (11-27%). In contrast to the pulp oils, seed oils had higher amount of polyunsaturated fatty acids (PUFAs) (65-72%). The fatty acid compositions of TAGs were very close to the compositions of corresponding seed and pulp oils. The major fatty acids in PLs of berry pulp/peel oils were oleic (20-40%), palmitic (17-27%), palmitoleic (10-22%) and linoleic (10%-20%) acids, whereas in seeds PLs, PUFAs prevailed. Comparing with the other lipid fractions the SEs had the highest contents of saturated fatty acids (SFAs). The fatty acid profiles of the FFA fractions were relatively similar to those of TAGs. All parts of the analyzed sea buckthorn berry cultivars (ssp. carpatica) exhibited higher oil content then the other European or Asiatic sea buckthorn subspecies. Moreover, the pulp/peel oils of ssp. carpatica were found to contain high levels of oleic acid and slightly lower amounts of linoleic and α-linolenic acids. The studied cultivars of sea buckthorn from Romania have proven to be potential sources of valuable oils.

  15. Absorption of flavonols derived from sea buckthorn (Hippophaë rhamnoides L.) and their effect on emerging risk factors for cardiovascular disease in humans.

    Science.gov (United States)

    Suomela, Jukka-Pekka; Ahotupa, Markku; Yang, Baoru; Vasankari, Tommi; Kallio, Heikki

    2006-09-20

    Sea buckthorn (Hippophaë rhamnoides L.) is a rich source of flavonols, especially isorhamnetin. Most prospective cohort studies have indicated some degree of inverse association between flavonoid intake and coronary heart disease. Animal and human studies suggest that sea buckthorn flavonoids may scavenge free radicals, lower blood viscosity, and enhance cardiac function. The effects of flavonol aglycones derived from sea buckthorn on the risk factors of cardiovascular disease as well as their absorption were studied in humans. The flavonols, ingested with oatmeal porridge, did not have a significant effect on the levels of oxidized low-density lipoprotein, C-reactive protein, and homocysteine, on the plasma antioxidant potential, or on the paraoxonase activity. Flavonols at two dosages in oatmeal porridge were rapidly absorbed, and a relatively small amount of sea buckthorn oil added to the porridge seemed to have increased the bioavailability of sea buckthorn flavonols consumed at the higher dose.

  16. ¹H NMR spectroscopy reveals the effect of genotype and growth conditions on composition of sea buckthorn (Hippophaë rhamnoides L.) berries.

    Science.gov (United States)

    Kortesniemi, Maaria; Sinkkonen, Jari; Yang, Baoru; Kallio, Heikki

    2014-03-15

    ¹H NMR spectroscopy and multivariate data analysis were applied to the metabolic profiling and discrimination of wild sea buckthorn (Hippophaë rhamnoides L.) berries from different locations in Finland (subspecies (ssp.) rhamnoides) and China (ssp. sinensis). Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed discrimination of the two subspecies and different growth sites. The discrimination of ssp. rhamnoides was mainly associated with typically higher temperature, radiation and humidity and lower precipitation in the south, yielding higher levels of O-ethyl β-d-glucopyranoside and d-glucose, and lower levels of malic, quinic and ascorbic acids. Significant metabolic differences (p 2,000 m) correlated with greater levels of malic and ascorbic acids in ssp. sinensis. The NMR metabolomics approach applied here is effective for identification of metabolites, geographical origin and subspecies of sea buckthorn berries. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Changes in content of major phenolic compounds during leaf development of sea buckthorn (Hippophaë rhamnoides L.

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    Anne Morgenstern

    2014-10-01

    Full Text Available Content of total phenolic compounds and antioxidant capacity (FRAP were investigated in the leaves of three sea buckthorn (Hippophaë rhamnoides L. cultivars: ‘Otradnaja’, ‘Gibrid Pertjika’ and ‘Ljubitelskaja’, at different dates. In addition, major phenolic compounds (catechin, kaempferol, quercetin, epigallocatechin, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, isorhamnetin-3-O-glucoside, rutin, gallic acid, procyanidin monomer glycoside, procyanidin dimer aglycone and hydrolyzable tannins I–III were determined in ‘Ljubitelskaja’. Antioxidant capacity and the content of total phenolic compounds fluctuated during April, May and June, and then increased until the end of July, when the highest values were observed. Total phenolic compounds were strongly correlated with FRAP. Levels were generally higher in ‘Ljubitelskaja’ than in ‘Otradnaja’ and ‘Gibrid Pertjika’. In ‘Ljubitelskaja’, hydrolyzable tannins I–III occurred in higher amounts than did any of the other studied phenolic compounds. The developmental stage of the leaves (harvesting date had a strong influence on content of phenolic compounds and should be carefully considered when harvesting sea buckthorn leaves for different purposes.

  18. Effects of latitude and weather conditions on proanthocyanidins in berries of Finnish wild and cultivated sea buckthorn (Hippophaë rhamnoides L. ssp. rhamnoides).

    Science.gov (United States)

    Yang, Wei; Laaksonen, Oskar; Kallio, Heikki; Yang, Baoru

    2017-02-01

    Sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) of varieties 'Terhi' and 'Tytti' and one of wild origin were cultivated in southern and northern Finland, harvested during 2007-2013. Proanthocyanidins (PAs) were analyzed with HILIC UPLC-ESI-MS. The southern and northern samples were separated in the partial least squares discriminant analysis model (four factors, R(2) 0.75, Q(2) 0.70). The total PAs were more abundant in berries from the north (610-970mg/100gDW) than in those from the south (340-450mg/100gDW) (p<0.05). In northern Finland, the length of the growth season as well as the temperature sum and radiation sum of the growth season until harvest were negatively correlated with the total PAs in all the samples but positively with PA oligomers in 'Tytti' and 'Terhi'. In southern Finland no respective correlations were seen. 'Terhi' and 'Tytti' had different trends in the content of total PA and oligomers in overripe stages. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats.

    Science.gov (United States)

    Diandong, Hou; Feng, Gu; Zaifu, Liang; Helland, Timothy; Weixin, Fu; Liping, Cai

    2016-03-01

    Chronic stress can suppress natural killer (NK) cell activity; this may also be related to the effect of stress on the neuroendocrine-immune network. Sea buckthorn (SBT) (Hippophae rhamnoides L.) is a thorny nitrogen fixing deciduous shrub, native to both Europe and Asia. It has been used as a medicinal plant in Tibetan and Mongolian traditional medicines. SBT has multifarious medical properties, including anti-fatigue as well as immunoregulatory effects. This study reports the effects of SBT oil with regard to the cytotoxicity and quantity of NK cells in the blood of a chronic-stress rat model, in addition to its mechanisms on the neuroendocrine-immune network. These results show that SBT oil, given by gavage to rats with chronic stress, could increase the following: body weight, NK cell quantities, and cytotoxicity, as well as the expression of perforin and granzyme B. The results also show that SBT oil in rats with chronic stress could suppress cortisol, ACTH, IL-1β and TNF-α levels, in addition to increasing 5-HT and IFN-γ serum levels. This leads to suggest that SBT oil, in rats with chronic stress, can increase NK cell cytotoxicity by upregulating the expression of perforin and granzyme B, thus causing associated effects of SBT oil on the neuroendocrine-immune network. © The Author(s) 2015.

  20. Chemical constituents of sea buckthorn (Hippophae rhamnoides L. fruit in populations of central Alborz Mountains in Iran

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    A. Kuhkheil

    2017-07-01

    Full Text Available Background and objectives: Hippophae rhamnoides L. known as sea buckthorn is a deciduous medicinal shrub belonging to Elaeagnaceae family. In this study, the most important chemical constituents of sea buckthornwere evaluated in wild populations of central Alborz Mountains in Iran during the growth season of 2014 and 2015. Methods: Phytochemical analysis of fruit pulp and seed oil traits was performed using different methods of chromatography such as spectrophotometry, HPLC and GC. Results: Based on the results of combined analysis of variance, significant (p≤0.01 difference ranges between populations were found in respect to fruit dry weight (21.32 to 32.03%, total phenolic compounds (20.78 to 34.60 mg/g, extractable tannin (1.99 to 5.74 mg/g, glucose (38.14 to 110.70 mg/g, total carotenoids (0.80 to 1.17 mg/g, lycopene (0.13 to 0.20 mg/g, β-carotene (0.18 to 0.26 mg/g, total flavonoids (0.98 to 2.80 mg/g, total soluble solids (TSS (11.85 to 31.50%, vitamin C (1.47 to 8.96 mg/g, seed oil content (4.51 to 7.91%, and two major unsaturated fatty acids including linoleic acid (28.71 to 37.44% and linolenic acid (21.52 to 28.28%. Factor analysis based on principal component analysis (PCA revealed most important traits with the highest correlation factor such as vitamin C, carbohydrates, TSS, fruit dry weight (FDW, and tannin for the first component. Conclusion: content of vitamin C was the main variable in chemical constituents for effective detection of original wild populations of central Alborz Mountains. Accordingly, sea buckthorn populations were divided into four main clusters and groups with high diversity based on their chemical compositions.

  1. Proanthocyanidins in Sea Buckthorn (Hippophaë rhamnoides L.) Berries of Different Origins with Special Reference to the Influence of Genetic Background and Growth Location.

    Science.gov (United States)

    Yang, Wei; Laaksonen, Oskar; Kallio, Heikki; Yang, Baoru

    2016-02-17

    Wild sea buckthorn berries from Finland (Hippophaë rhamnoides ssp. rhamnoides) and China (ssp. sinensis) as well as berries of two varieties of ssp. rhamnoides cultivated in Finland and five of ssp. mongolica cultivated in Canada were compared on the basis of the content and composition of proanthocyanidins (PAs). Among all of the samples, only B-type PAs were found. The contents of dimeric, trimeric, tetrameric, and total PAs were in the range of 1.4-8.9, 1.3-9.5, 1.0-7.1, and 390-1940 mg/100 g of dry weight, respectively. The three subspecies were separated by three validated factors (R(2), 0.724; Q(2), 0.677) in the partial least squares discriminant analysis model. Significant differences in total PAs were found between the ssp. rhamnoides and mongolica samples (p < 0.05). In ssp. rhamnoides, samples grown in northern Finland were characterized by a high amount of total PAs, typically 2-3 times higher than that in the level found in southern Finland. In ssp. sinensis, altitude did not have a systematic effect on the PA composition, suggesting the significance of the interaction between genetic background and growth location.

  2. Sensory profile of ethyl β-d-glucopyranoside and its contribution to quality of sea buckthorn (Hippophaë rhamnoides L.).

    Science.gov (United States)

    Ma, Xueying; Laaksonen, Oskar; Heinonen, Jari; Sainio, Tuomo; Kallio, Heikki; Yang, Baoru

    2017-10-15

    The contribution of ethyl β-d-glucopyranoside (EG) to sensory profile of sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) was studied. The content of natural existing EG varied between 0.6 and 19.8g/L in the sea buckthorn studied. The taste threshold of pure EG was estimated in water solution as 1.1±1.3g/L, and the suprathreshold aqueous EG solution (5.0g/L) was perceived mainly as bitter. In aqueous sugar-acid mixtures, acids were the major contributors to sourness and astringency, whereas the added EG or sugars showed little impact on the overall sensory profile. EG addition increased the bitterness of sea buckthorn juice. The sensory profiles of the juices were dominated by intense sourness correlating with the malic acid content. The bitterness of the juices correlated with the EG content as well as the ratios EG/acids and EG/sugars. EG content shall be considered when selecting raw materials for industrial processing. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. The fibres and polyphenols in sea buckthorn (Hippophaë rhamnoides) extraction residues delay postprandial lipemia.

    Science.gov (United States)

    Linderborg, Kaisa M; Lehtonen, Henna-Maria; Järvinen, Riikka; Viitanen, Matti; Kallio, Heikki

    2012-06-01

    The triacylglycerol (TAG) response to fatty meals containing dried and crushed berries or berry extraction residues was studied in a postprandial cross-over study with healthy normal-weight male volunteers. The berry material included sea buckthorn berries, sea buckthorn CO₂ extraction residue (CO₂-sea buckthorn) and sea buckthorn or black currant CO₂ and ethanol extraction residue (CO₂-EtOH-sea buckthorn, CO₂-EtOH-black currant). Extraction residues were used in order to advance the potential use of valuable side stream components containing polyphenols and fibre as human food. Compared to the berry-depleted control, all berry meals delayed lipemia, whereas there were no differences in the total area under the TAG response curve. The lipemic delay largely derived from the fibre rather than from the polyphenols. Even so, the effect of polyphenols may be complementary since sea buckthorn and CO₂-sea buckthorn showed significant differences in relation to control in a wider range of TAG areas than polyphenol-depleted CO₂-EtOH-sea buckthorn.

  4. Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides, Apricot Pulp and Apricot Kernel (Prunus armeniaca

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    Elena Andreea Pop

    2015-11-01

    Full Text Available A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L. and apricot fruits (Prunus armeniaca L. are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits. The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC  method.  Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection. The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg than in apricot fruits (1.09 mg/kg while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg. Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.

  5. Impact of electron beam irradiation on quality of sea buckthorn (Hippophae rhamnoides L.) oil.

    Science.gov (United States)

    Nemţanu, Monica R; Braşoveanu, Mirela

    2016-03-30

    Sea buckthorn oil is a valuable product that can be incorporated into daily foodstuffs, cosmetics or pharmaceuticals. The effect of accelerated electron irradiation up to 8 kGy on quality characteristics of sea buckthorn oil was investigated in this study. Irradiation had no significant influence on phenolic content. Conversely, carotenoid content, antioxidant activity, and oxidative status suffered alterations as the irradiation dose increased. Although no colour changes were visible for oil irradiated up to 3 kGy, the total colour difference indicated clearly changes that involved a two-step pattern associated with slow degradation of oil colour up to 3 kGy, followed by a fast degradation up to 8 kGy. Some changes of the oil spectral features related to the frequency and intensity of some bands have been found after irradiation, indicating an alteration of the structural integrity induced by irradiation. The present investigation may be a useful starting point for irradiation processing of food or non-food matrices containing sea buckthorn oil. Thus, sea buckthorn oil safety can be ensured with minimal undesirable changes in its quality by applying irradiation doses up to 3 kGy, which allow control of the microbial contamination depending on microorganism type and initial microbial load. © 2015 Society of Chemical Industry.

  6. Fatty acid, Tocopherol and Sterol Composition in Sea buckthorn (Hippophae rhamnoides L. of Mongolia

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    Ch Otgonbayar

    2014-09-01

    Full Text Available The content and composition of lipids isolated from seed and pulp of sea buckthorn were investigated. Fatty acids and sterols were analyzed by CGC while tocopherols were analyzed by HPLC. 12.67% glyceride was found in the seed. The oil of sea buckthorn seed oil showed low levels of saturated fatty acids in comparison with the buckthorn pulp oil. Palmitic (7.13%, oleic (15.85%, linoleic (36.9% and linolenic acids (31.11% predominated in the seed oil. Palmitic (29.17%, palmitoleic (32.86%, oleic (4.92%, vaccenic (9.35% and linoleic (16.08% fatty acid was dominating in the pulp oil. The primary tocopherol of sea buckthorn seed and pulp oil were α- tocopherol and γ-tocopherol (46.54mg/100g, 59.02mg/100g. Seed oil contains more (94.34mg/100g total sterols than pulp (90.25mg/100g oil.DOI: http://dx.doi.org/10.5564/mjc.v12i0.187 Mongolian Journal of Chemistry Vol.12 2011: 126-130 

  7. NMR metabolomics demonstrates phenotypic plasticity of sea buckthorn (Hippophaë rhamnoides) berries with respect to growth conditions in Finland and Canada.

    Science.gov (United States)

    Kortesniemi, Maaria; Sinkkonen, Jari; Yang, Baoru; Kallio, Heikki

    2017-03-15

    The berries of sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) cultivars 'Terhi' and 'Tytti' were studied with respect to their growth location, 60° and 68°N latitude in Finland and 46°N in Canada, using 1 H NMR metabolomics. The berries of 'Terhi' were characterised by stronger signals of quinic acid, while 'Tytti' had higher levels of O-ethyl β-d-glucopyranoside. The metabolic profile of the northernmost berries was distinctly different from those grown in southern Finland or Canada. Berries from northern Finland had relatively higher levels of quinic acid, glucose, l-quebrachitol and ascorbic acid. Ethyl glucoside was shown to accumulate by several fold at the late stage of maturation in the south as it correlated with degree days (r=0.63) and global radiation (r=0.59), but not in the north. The variance in the composition of the sea buckthorn berries demonstrates plasticity in the acclimatisation to growth environments. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Drought hardiness of sea buckthorn (Hippophae rhamnoides L. varieties in conditions of Orel region.

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    З. Є. Ожерельєва

    2011-05-01

    Full Text Available The estimation of drought hardiness of sea buckthorn varieties of different environmental and geographical origin was made in the field and laboratory conditions. In late August the drought hardiness was estimated according to leaf injures and abscission in the field conditions. In the laboratory conditions were determined the main characteristics of water regime: tissue water saturation, water deficit, leaf water retaining ability. As a consequence, the studied varieties were divided into groups with different drought hardiness degree.

  9. Sea buckthorn (Hippophae rhamnoides) proanthocyanidins inhibit in vitro enzymatic hydrolysis of protein.

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    Arimboor, Ranjith; Arumughan, C

    2011-08-01

    Interactions of phenolics with other food constituents and digestive enzymes are likely to have interference with the digestion and bioavailability of food and phenolics. In this study the effect of sea buckthorn proanthocyanidins on in vitro digestion of protein was evaluated. Optimization of the extraction conditions showed that maximum recovery of sea buckthorn proanthocyanidins was obtained with acidified acetone; water mixture (60% to 70%, v/v). Crude proanthocyanidin extracts thus prepared were purified using sephadex gel column chromatography and their average degree of polymerization and the effects on enzymatic hydrolysis of bovine serum albumin as influenced by their protein precipitation capacities were studied. Average degree of polymerization of proanthocyanidins in berry pulp, kernel, seed coat, and leaves was 7.4, 5.6, 8.2, and 10.6, respectively. The EC50 values for the protein precipitation by the PA of berry pulp, kernel seed coat, and leaves were 44.2, 44.1, 65.8, and 39.8 μg, respectively. Relative enzymatic hydrolysis of the protein-proanthocyanidin complexes was 44.1% to 60.3% for pepsin and 57.5% to 67.7% for trypsin. Interactions of sea buckthorn proanthocyanidins with food proteins and digestive enzymes might alter the protein digestibility and phenolic bioavailabilty. © 2011 Institute of Food Technologists®

  10. Metabolite profiling and expression analysis of flavonoid, vitamin C and tocopherol biosynthesis genes in the antioxidant-rich sea buckthorn (Hippophae rhamnoides L.).

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    Fatima, Tahira; Kesari, Vigya; Watt, Ian; Wishart, David; Todd, James F; Schroeder, William R; Paliyath, Gopinadhan; Krishna, Priti

    2015-10-01

    In this study, phenolic compounds were analyzed in developing berries of four Canadian grown sea buckthorn (Hippophae rhamnoides L.) cultivars ('RC-4', 'E6590', 'Chuyskaya' and 'Golden Rain') and in leaves of two of these cultivars. Among phenolic acids, p-coumaric acid was the highest in berries, while gallic acid was predominant in leaves. In the flavonoid class of compounds, myricetin/rutin, kaempferol, quercetin and isorhamnetin were detected in berries and leaves. Berries of the 'RC-4' cultivar had approximately ⩾ 2-fold higher levels of myricetin and quercetin at 17.5mg and 17.2 mg/100 g FW, respectively, than the other cultivars. The flavonoid content in leaves was considerably more than in berries with rutin and quercetin levels up to 135 mg and 105 mg/100 g FW, respectively. Orthologs of 15 flavonoid biosynthesis pathway genes were identified within the transcriptome of sea buckthorn mature seeds. Semi-quantitative RT-PCR analysis of these genes in developing berries indicated relatively higher expression of genes such as CHS, F3'H, DFR and LDOX in the 'RC-4' cultivar than in the 'Chuyskaya' cultivar. Vitamin C levels in ripened berries of the Canadian cultivars were on the high end of the concentration range reported for most other sea buckthorn cultivars. Orthologs of genes involved in vitamins C and E biosynthesis were also identified, expanding the genomic resources for this nutritionally important plant. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Soil microbial communities and enzyme activities in sea-buckthorn (Hippophae rhamnoides plantation at different ages.

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    Miao Yang

    Full Text Available The aim of this study was to assess the impact of forest age and season on the soil microbial community and enzyme activities in sea-buckthorn plantation system and to determine the relative contributions to soil microbial properties. Soil sampling was carried out in the dry season (April and wet season (September in four areas, including: abandoned farmland (NH, an 8-year- old plantation (young plantation, 8Y, a 13-year-old plantation (middle-aged plantation, 13Y, and an 18-year-old plantation (mature plantation, 18Y. The results showed that forest age and season have a significant effect on soil microbial community structure and enzyme activities. The total, bacterial, fungal, Gram-negative (G+, and Gram-positive (G- PLFAs increased gradually with forest age, with the highest values detected in 18Y. All the detected enzyme activities showed the trend as a consequence of forest age. The microbial PLFAs and soil enzyme activities were higher in the wet season than the dry season. However, there were no significant interactions between forest age and season. A Correlation analysis suggested that soil microbial communities and enzyme activities were significantly and positively correlated with pH, total nitrogen (TN and available phosphorus (AP. Season had a stronger influence on soil microbial communities than forest age. In general, sea-buckthorn plantations establishment might be a potential tool for maintaining and increasing soil fertility in arid and semi-arid regions.

  12. Soil microbial communities and enzyme activities in sea-buckthorn (Hippophae rhamnoides) plantation at different ages.

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    Yang, Miao; Yang, Dan; Yu, Xuan

    2018-01-01

    The aim of this study was to assess the impact of forest age and season on the soil microbial community and enzyme activities in sea-buckthorn plantation system and to determine the relative contributions to soil microbial properties. Soil sampling was carried out in the dry season (April) and wet season (September) in four areas, including: abandoned farmland (NH), an 8-year- old plantation (young plantation, 8Y), a 13-year-old plantation (middle-aged plantation, 13Y), and an 18-year-old plantation (mature plantation, 18Y). The results showed that forest age and season have a significant effect on soil microbial community structure and enzyme activities. The total, bacterial, fungal, Gram-negative (G+), and Gram-positive (G-) PLFAs increased gradually with forest age, with the highest values detected in 18Y. All the detected enzyme activities showed the trend as a consequence of forest age. The microbial PLFAs and soil enzyme activities were higher in the wet season than the dry season. However, there were no significant interactions between forest age and season. A Correlation analysis suggested that soil microbial communities and enzyme activities were significantly and positively correlated with pH, total nitrogen (TN) and available phosphorus (AP). Season had a stronger influence on soil microbial communities than forest age. In general, sea-buckthorn plantations establishment might be a potential tool for maintaining and increasing soil fertility in arid and semi-arid regions.

  13. In-tube extraction and GC-MS analysis of volatile components from wild and cultivated sea buckthorn (Hippophae rhamnoides L. ssp. Carpatica) berry varieties and juice.

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    Socaci, Sonia A; Socaciu, Carmen; Tofană, Maria; Raţi, Ioan V; Pintea, Adela

    2013-01-01

    The health benefits of sea buckthorn (Hippophae rhamnoides L.) are well documented due to its rich content in bioactive phytochemicals (pigments, phenolics and vitamins) as well as volatiles responsible for specific flavours and bacteriostatic action. The volatile compounds are good biomarkers of berry freshness, quality and authenticity. To develop a fast and efficient GC-MS method including a minimal sample preparation technique (in-tube extraction, ITEX) for the discrimination of sea buckthorn varieties based on their chromatographic volatile fingerprint. Twelve sea buckthorn varieties (wild and cultivated) were collected from forestry departments and experimental fields, respectively. The extraction of volatile compounds was performed using the ITEX technique whereas separation and identification was performed using a GC-MS QP-2010. Principal component analysis (PCA) was applied to discriminate the differences among sample composition. Using GC-MS analysis, from the headspace of sea buckthorn samples, 46 volatile compounds were separated with 43 being identified. The most abundant derivatives were ethyl esters of 2-methylbutanoic acid, 3-methylbutanoic acid, hexanoic acid, octanoic acid and butanoic acid, as well as 3-methylbutyl 3-methylbutanoate, 3-methylbutyl 2-methylbutanoate and benzoic acid ethyl ester (over 80% of all volatile compounds). Principal component analysis showed that the first two components explain 79% of data variance, demonstrating a good discrimination between samples. A reliable, fast and eco-friendly ITEX/GC-MS method was applied to fingerprint the volatile profile and to discriminate between wild and cultivated sea buckthorn berries originating from the Carpathians, with relevance to food science and technology. Copyright © 2013 John Wiley & Sons, Ltd.

  14. Effect of monospecific and mixed sea-buckthorn (Hippophae rhamnoides) plantations on the structure and activity of soil microbial communities.

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    Yu, Xuan; Liu, Xu; Zhao, Zhong; Liu, Jinliang; Zhang, Shunxiang

    2015-01-01

    This study aims to evaluate the effect of different afforestation models on soil microbial composition in the Loess Plateau in China. In particular, we determined soil physicochemical properties, enzyme activities, and microbial community structures in the top 0 cm to 10 cm soil underneath a pure Hippophae rhamnoides (SS) stand and three mixed stands, namely, H. rhamnoides and Robinia pseucdoacacia (SC), H. rhamnoides and Pinus tabulaeformis (SY), and H. rhamnoides and Platycladus orientalis (SB). Results showed that total organic carbon (TOC), total nitrogen, and ammonium (NH4(+)) contents were higher in SY and SB than in SS. The total microbial biomass, bacterial biomass, and Gram+ biomass of the three mixed stands were significantly higher than those of the pure stand. However, no significant difference was found in fungal biomass. Correlation analysis suggested that soil microbial communities are significantly and positively correlated with some chemical parameters of soil, such as TOC, total phosphorus, total potassium, available phosphorus, NH4(+) content, nitrate content (NH3(-)), and the enzyme activities of urease, peroxidase, and phosphatase. Principal component analysis showed that the microbial community structures of SB and SS could clearly be discriminated from each other and from the others, whereas SY and SC were similar. In conclusion, tree species indirectly but significantly affect soil microbial communities and enzyme activities through soil physicochemical properties. In addition, mixing P. tabulaeformis or P. orientalis in H. rhamnoides plantations is a suitable afforestation model in the Loess Plateau, because of significant positive effects on soil nutrient conditions, microbial community, and enzyme activities over pure plantations.

  15. Effect of monospecific and mixed sea-buckthorn (Hippophae rhamnoides plantations on the structure and activity of soil microbial communities.

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    Xuan Yu

    Full Text Available This study aims to evaluate the effect of different afforestation models on soil microbial composition in the Loess Plateau in China. In particular, we determined soil physicochemical properties, enzyme activities, and microbial community structures in the top 0 cm to 10 cm soil underneath a pure Hippophae rhamnoides (SS stand and three mixed stands, namely, H. rhamnoides and Robinia pseucdoacacia (SC, H. rhamnoides and Pinus tabulaeformis (SY, and H. rhamnoides and Platycladus orientalis (SB. Results showed that total organic carbon (TOC, total nitrogen, and ammonium (NH4(+ contents were higher in SY and SB than in SS. The total microbial biomass, bacterial biomass, and Gram+ biomass of the three mixed stands were significantly higher than those of the pure stand. However, no significant difference was found in fungal biomass. Correlation analysis suggested that soil microbial communities are significantly and positively correlated with some chemical parameters of soil, such as TOC, total phosphorus, total potassium, available phosphorus, NH4(+ content, nitrate content (NH3(-, and the enzyme activities of urease, peroxidase, and phosphatase. Principal component analysis showed that the microbial community structures of SB and SS could clearly be discriminated from each other and from the others, whereas SY and SC were similar. In conclusion, tree species indirectly but significantly affect soil microbial communities and enzyme activities through soil physicochemical properties. In addition, mixing P. tabulaeformis or P. orientalis in H. rhamnoides plantations is a suitable afforestation model in the Loess Plateau, because of significant positive effects on soil nutrient conditions, microbial community, and enzyme activities over pure plantations.

  16. Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model.

    Science.gov (United States)

    Ito, Hiroshi; Asmussen, Sven; Traber, Daniel L; Cox, Robert A; Hawkins, Hal K; Connelly, Rhykka; Traber, Lillian D; Walker, Timothy W; Malgerud, Erik; Sakurai, Hiroyuki; Enkhbaatar, Perenlei

    2014-05-01

    To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p<0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p<0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p<0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds. Copyright © 2013 Elsevier Ltd and ISBI. All rights reserved.

  17. Vitamin C content in sea buckthorn berries (Hippophaë rhamnoides L. ssp. rhamnoides) and related products: a kinetic study on storage stability and the determination of processing effects.

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    Gutzeit, D; Baleanu, G; Winterhalter, P; Jerz, G

    2008-11-01

    The primary vitamin in sea buckthorn berries is vitamin C containing values of approximately 400 mg/100 g. Processing effects were investigated during juice and concentrate production from sea buckthorn berries (Hippophaë rhamnoides) and storage stability of juices was determined for up to 7 d using berries and juices from 2 different growing areas. During industrial juice production the technological processing of the berries caused a loss of about 5% to 11% total ascorbic acid (TAA) in the generated juice. The production of the concentrated juice resulted in 50% depletion of TAA. Sea buckthorn berries and juice were stored at 6, 25, and 40 degrees C for up to 7 d to investigate the temperature effects on TAA during storage. Analysis of kinetic data suggested that the degradation follows a 1st-order model. The results of the experiments showed that storage of sea buckthorn juices for 7 d at cold temperature (6 degrees C) already resulted in a degradation of TAA of about 11% to 12%.

  18. Flavonol glycosides in berries of two major subspecies of sea buckthorn (Hippophaë rhamnoides L.) and influence of growth sites.

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    Ma, Xueying; Laaksonen, Oskar; Zheng, Jie; Yang, Wei; Trépanier, Martin; Kallio, Heikki; Yang, Baoru

    2016-06-01

    Flavonol glycosides of wild sea buckthorn (Hippophaë rhamnoides ssp. sinensis) berries from China and cultivated berries (H. rhamnoides ssp. mongolica) from Finland and Canada were identified and quantified. Twenty-six flavonol glycosides were found with isorhamnetin and quercetin as the major aglycones. The contents of flavonol glycosides ranged 23-250 mg/100 g fresh berries and were significantly higher in the berries of ssp. sinensis than in those of ssp. mongolica. Among the cultivars of ssp. mongolica, the berries of 'Oranzhevaya' had the lowest (23 mg/100 g) content, and those of 'Prevoshodnaya' the highest content of flavonol glycosides (80 mg/100 g). Within the ssp. mongolica, the samples from Kittilä (Northern Finland) had higher levels of most flavonol glycosides than those from Turku (Southern Finland) and Québec. Among the ssp. sinensis berries of different growth sites, increasing trends were detected in the contents of most of the compounds as the altitude increased and as the latitude decreased. The wild berries (ssp. sinensis) from Sichuan had remarkably high contents and unique profiles of flavonol glycosides. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Proanthocyanidins in wild sea buckthorn (Hippophaë rhamnoides) berries analyzed by reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography with UV and MS detection.

    Science.gov (United States)

    Kallio, Heikki; Yang, Wei; Liu, Pengzhan; Yang, Baoru

    2014-08-06

    A rapid and sensitive method for profiling of proanthocyanidins (PAs) of sea buckthorn (Hippophaë rhamnoides) berries was established based on aqueous, acidified acetone extraction. The extract was purified by Sephadex column chromatography and analyzed using reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography (HILIC). Negative ion electrospray ionization mass spectrometry (ESI-MS) in single ion recording (SIR) and full scan modes combined with UV detection were used to define the combinations and ratios of PA oligomer classes. PAs with degree of polymerization from 2 to 11 were detected by HILIC-ESI-MS. Quantification of dimeric, trimeric, and tetrameric PAs was carried out with ESI-MS-SIR, and their molar proportions were 40, 40, and 20%, respectively. Only B-type PAs were found, and (epi)gallocatechins were the main monomeric units. More than 60 combinations of (epi)catechins and (epi)gallocatechins of proanthocyanidin dimers and trimers were found. A majority of the PAs were shown to be higher polymers based on the HILIC-UV analysis.

  20. Study on the Sea-Buckthorn (Hippophae rhamnoides L. Preparation Forms Destined to its Nutritio-Pharmaceutical Use

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    Carmen Georgeta Dumitrescu (Manole

    2016-11-01

    Full Text Available In conducting this research we started from the multiple uses of sea-buckthorn in our everyday life. In addition to the positive effect of sea-buckthorn plant on the environment, especially by fixing the soil, almost all parts of this miraculous shrub have therapeutically effect (fruits, leaves, shoots etc. and can be consumed in various forms. Aim of this paper is to present the nutritional value of sea-buckthorn, recipes most frequently used and the effects that they have on people. In order to achieve all these, materials from the scientific literature were used, as well as an own research, carried out during a year, on a total number of 50 people. Results prove that the sea-buckthorn must be introduced, in greater extent, in our daily diet, as it improves human health.

  1. Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.).

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    Kruczek, Michał; Swiderski, Adam; Mech-Nowak, Aleksandra; Król, Katarzyna

    2012-01-01

    Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.

  2. The Use of Sea Buckthorn (Hippophae rhamnoides L. and Milk Thistle (Silybum marianum L. in Alloxan Induced Diabetes Mellitus in Rats

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    Florin Muselin

    2016-05-01

    Full Text Available The study emphasize the effect of H. rhamnoides and S. marianum 6% aqueous extract on blood sugar level in Alloxan induced diabetes mellitus in rats. The rats (200g and 3 months age were divided in five groups: one non-diabetic control and four experimental groups with induced diabetes mellitus after 40 mg/kg b.w. intravenous administration of 2% Alloxan. One group was kept as diabetic control and in the other three groups was administered 6% aqueous extracts of H. rhamnoides, S. marianum or a combination of the extracts during seven weeks. The stronger effect was obtained in case of H. rhamnoides (Sea buckthorn extract administration followed by the S. marianum (Milk thistle extract. The combination of the two extracts proven to have a stronger effect than the extracts given separately.

  3. Effects of genotype, latitude, and weather conditions on the composition of sugars, sugar alcohols, fruit acids, and ascorbic acid in sea buckthorn (Hippophaë rhamnoides ssp. mongolica) berry juice.

    Science.gov (United States)

    Zheng, Jie; Yang, Baoru; Trépanier, Martin; Kallio, Heikki

    2012-03-28

    Sea buckthorn berries (Hippophaë rhamnoides ssp. mongolica) of nine varieties were collected from three growth locations in five inconsecutive years (n = 152) to study the compositional differences of sugars, sugar alcohols, fruit acids, and ascorbic acid in berries of different genotypes. Fructose and glucose (major sugars) were highest in Chuiskaya and Vitaminaya among the varieties studied, respectively. Malic acid and quinic acid (major acids) were highest in Pertsik and Vitaminaya, respectively. Ascorbic acid was highest in Oranzhevaya and lowest in Vitaminaya. Berry samples of nine varieties collected from two growth locations in five years (n = 124) were combined to study the effects of latitude and weather conditions on the composition of H. rhamnoides ssp. mongolica. Sea buckthorn berries grown at lower latitude had higher levels of total sugar and sugar/acid ratio and a lower level of total acid and were supposed to have better sensory properties than those grown at higher latitude. Glucose, quinic acid, and ascorbic acid were hardly influenced by weather conditions. The other components showed various correlations with temperature, radiation, precipitation, and humidity variables. In addition, fructose, sucrose, and myo-inositol correlated positively with each other and showed negative correlation with malic acid on the basis of all the samples studied (n = 152).

  4. Isolation and identification of sea buckthorn (Hippophae rhamnoides) phenolics with antioxidant activity and α-glucosidase inhibitory effect.

    Science.gov (United States)

    Kim, Ju-Sung; Kwon, Yong-Soo; Sa, Yeo-Jin; Kim, Myong-Jo

    2011-01-12

    This study was performed to evaluate the antioxidant and α-glucosidase inhibitory effects from the extract, fractions, and isolated compounds of sea buckthorn leaves. Six compounds, kaempferol-3-O-β-D-(6''-O-coumaryl) glycoside, 1-feruloyl-β-D-glucopyranoside, isorhamnetin-3-O-glucoside, quercetin 3-O-β-D-glucopyranoside, quercetin 3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside, and isorhamnetin-3-O-rutinoside, were isolated from sea buckthorn leaf extracts. The butanol fraction (EC(50) = 1.81 μg/mL) along with quercetin 3-O-β-D-glucopyranoside (EC(50) = 1.86 μg/mL) had a higher DPPH radical-scavenging activity and showed stronger reducing power (OD(700) = 1.83 and 1.78, respectively). The butanol fraction (477 mg GAE/g) contained the highest amount of phenolic compounds and also the most powerful α-glucosidase inhibitory effect (86%) at 5 μg/mL. The results indicate that sea buckthorn leaf extracts could potentially be used for food additives and the development of useful natural compounds.

  5. Thermal stability of the complex formed between carotenoids from sea buckthorn (Hippophae rhamnoides L.) and bovine β-lactoglobulin

    Science.gov (United States)

    Aprodu, Iuliana; Ursache, Florentina-Mihaela; Turturică, Mihaela; Râpeanu, Gabriela; Stănciuc, Nicoleta

    2017-02-01

    Sea buckthorn has gained importance as a versatile nutraceutical, due to its high nutritive value in terms of carotenoids content. β-Lactoglobulin (β-LG) is a natural carrier for various bioactive compounds. In this study, the effect of thermal treatment in the temperature range of 25 to 100 °C for 15 min on the complex formed by β-LG and carotenoids from sea buckthorn was reported, based on fluorescence spectroscopy, molecular docking and molecular dynamics simulation results. Also, the berries extracts were analyzed for their carotenoids content. The chromatographic profile of the sea buckthorn extracts revealed the presence of zeaxanthin and β-carotene, as major compounds. The Stern-Volmer constants and binding parameters between β-LG and β-carotene were estimated based on quenching experiments. When thermally treating the β-LG-carotenoids mixtures, an increase in intrinsic and extrinsic fluorescence intensity up to 90 °C was observed, together with blue-shifts in maximum emission in the lower temperature range and red-shifts at higher temperature. Based on fluorescence spectroscopy results, the unfolding of the protein molecules at high temperature was suggested. Detailed information obtained at atomic level revealed that events taking place in the complex heated at high temperature caused important changes in the β-carotene binding site, therefore leading to a more thermodynamically stable assembly. This study can be used to understand the changes occurring at molecular level that could help food operators to design new ingredients and functional foods, and to optimize the processing methods in order to obtain healthier food products.

  6. Research on the Sea-Buckthorn (Hippophae rhamnoides L. Eco-Restoration Value in Avoiding Erosion and Supporting Soil Fixation

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    Carmen Georgeta Dumitrescu (Manole

    2016-11-01

    Full Text Available This paper approaches an issue of real importance, namely the sea-buckthorn influence on the areas attacked by erosion. We consider that this effect is mainly a result of its extremely developed root system, as well as of the nodosities formation on the roots. The study was conducted over a period of 3 years (2011-2013 in two landslides areas from Prahova and Dambovita counties, from where were collected 5 plants (from each area in 3 repetitions. Calculating the averages regarding the plant roots depth, but also the number of root suckers and nodosities, can be easily deduced the role of these plants in soil fixation, hence their eco-restoration value.

  7. Optimization of subcritical water extraction parameters of antioxidant polyphenols from sea buckthorn (Hippophaë rhamnoides L.) seed residue.

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    Gong, Ying; Zhang, Xiaofei; He, Li; Yan, Qiuli; Yuan, Fang; Gao, Yanxiang

    2015-03-01

    Polyphenols was extracted with subcritical water from the sea buckthorn seed residue (after oil recovery), and the extraction parameters were optimized using response surface methodology (RSM). The independent processing variables were extraction temperature, extraction time and the ratio of water to solid. The optimal extraction parameters for the extracts with highest ABTS radical scavenging activity were 120 °C, 36 min and the water to solid ratio of 20, and the maximize antioxidant capacity value was 32.42 mmol Trolox equivalent (TE)/100 g. Under the optimal conditions, the yield of total phenolics, total flavonoids and proanthocyanidins was 36.62 mg gallic acid equivalents (GAE)/g, 19.98 mg rutin equivalent (RE)/g and 10.76 mg catechin equivalents (CE)/g, respectively.

  8. Phytochemical composition and antibacterial activity of the essential oils from different parts of sea buckthorn (Hippophae rhamnoides L.

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    Xuan-Feng Yue

    2017-04-01

    Full Text Available Essential oils from the seed, pulp, and leaf of sea buckthorn were obtained with hydrodistillation, and their phytochemical composition was analyzed through gas chromatography–mass spectrometry. Furthermore, the antibacterial activity of the oils was tested on five food-borne bacteria by spectrometry and evaluated in terms of minimum inhibitory concentration. The results indicate that the composition of all essential oils is dominated by free fatty acids, esters, and alkanes. Minimum inhibitory concentration values on each bacterium were obtained for oils from different parts. The oils from different parts exhibited nearly equal inhibitory effect on Staphylococcus aureus. The pulp oil was found to be the most effective for the rest of bacteria tested except Escherichia coli, on which seed oil shows twice the inhibitory effect to that of leaf or pulp oil. Three natural inhibitory examples were found comparable with or even better than the positive control: pulp oil on Bacillus subtilis, and pulp oil and leaf oil on Bacillus coagulans.

  9. Colonic fermentation of polyphenolics from Sea buckthorn (Hippophae rhamnoides) berries: Assessment of effects on microbial diversity by Principal Component Analysis.

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    Attri, Sampan; Sharma, Kavita; Raigond, Pinky; Goel, Gunjan

    2018-03-01

    The present study investigates the stability of polyphenolic in Sea buckthorn berries juice (SBJ) during different phases of digestion and its effect on colonic microbial diversity. At each stage, the Total polyphenolic content (TPC), Total antioxidant activity (TAA) and polyphenolic profile was determined. A 1.64 and 2.20 folds increase in TPC with 4.88 and 9.61 folds increase in TAA were observed during gastric and small intestine digestion (p<0.05) with the release of quercetin from food matrix. The digestion resulted in deformation of intact crystalline structure as indicated by scanning electron micrographs. The colonic fermentation resulted in an increase in quercetin, caffeic acid with decrease in rutin and chlorogenic acid after 36h of fermentation (p<0.05). The Shannon diversity index (H) of beneficial groups including Lactic acid bacteria (LAB), Bacteroides/Prevotella and Bifidobacteria was increased by 35%, 71% and 17%, respectively (p<0.05). The PCA analysis indicated that the presence and digestion of polyphenolics promote the proliferation of Bacteroides/Prevotella group as well as Lactic acid bacteria and Bifidobacteria. The results suggest that SBJ is good source of prebiotic substrate in terms of the proliferation of beneficial gut microbiota. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Phytochemical composition and antibacterial activity of the essential oils from different parts of sea buckthorn (Hippophae rhamnoides L.).

    Science.gov (United States)

    Yue, Xuan-Feng; Shang, Xiao; Zhang, Zhi-Juan; Zhang, Yan-Ni

    2017-04-01

    Essential oils from the seed, pulp, and leaf of sea buckthorn were obtained with hydrodistillation, and their phytochemical composition was analyzed through gas chromatography-mass spectrometry. Furthermore, the antibacterial activity of the oils was tested on five food-borne bacteria by spectrometry and evaluated in terms of minimum inhibitory concentration. The results indicate that the composition of all essential oils is dominated by free fatty acids, esters, and alkanes. Minimum inhibitory concentration values on each bacterium were obtained for oils from different parts. The oils from different parts exhibited nearly equal inhibitory effect on Staphylococcus aureus. The pulp oil was found to be the most effective for the rest of bacteria tested except Escherichia coli, on which seed oil shows twice the inhibitory effect to that of leaf or pulp oil. Three natural inhibitory examples were found comparable with or even better than the positive control: pulp oil on Bacillus subtilis, and pulp oil and leaf oil on Bacillus coagulans. Copyright © 2016. Published by Elsevier B.V.

  11. Pathology of ochratoxin A-induced nephrotoxicity in Japanese quail and its protection by sea buckthorn (Hippophae rhamnoides L.).

    Science.gov (United States)

    Patial, V; Asrani, R K; Patil, R D; Ledoux, D R; Rottinghaus, G E

    2013-12-01

    The present study was designed to study the protective effect of sea buckthorn (SBT) against renal damage induced by ochratoxin A (OTA) in Japanese quail. Day-old quail chicks were divided into six groups and fed a basal quail chick mash containing 2% SBT leaf powder (group SX), OTA at a dietary level of 3 ppm (group OX), 25 ppm L-beta-phenylalanine (Phe) plus 3 ppm OTA (group OP), 2% dietary level of SBT leaf powder plus 3 ppm OTA (group OS), SBT leaf extract at a level of 10%/L of drinking water plus 3 ppm OTA (group OSS), and a standard toxin-free feed (group CX, control) for 21 days. OTA at 3 ppm level in diet grossly revealed mild to moderate renal swelling in OX birds, and the severity was less in the case of OS, OSS, and OP birds. Microscopically, degenerative, necrotic, and inflammatory changes were observed in OX birds, but the changes were less severe in OS, OSS, and OP birds. Ultrastructural studies revealed remarkable and consistent changes in the proximal convoluted tubules (PCTs), with severe damage of mitochondria and endoplasmic reticulum in OX birds, whereas SBT-treated birds (groups OS, OSS) had mild changes in mitochondria. A moderate to marked increase in number of peroxisomes in the cytoplasm of PCTs was a consistent finding in the Phe- and SBT-treated groups kept on OTA in comparison to the group fed OTA alone. In conclusion, the inclusion of 2% SBT leaf powder in feed and SBT leaf extract in water provided partial protection against OTA-induced nephropathy in Japanese quail.

  12. UV radiation-induced skin aging in hairless mice is effectively prevented by oral intake of sea buckthorn (Hippophae rhamnoides L.) fruit blend for 6 weeks through MMP suppression and increase of SOD activity.

    Science.gov (United States)

    Hwang, In Sik; Kim, Ji Eun; Choi, Sun Il; Lee, Hye Ryun; Lee, Young Ju; Jang, Min Ju; Son, Hong Ju; Lee, Hee Seob; Oh, Chung Hun; Kim, Bae Hwan; Lee, Sang Hak; Hwang, Dae Youn

    2012-08-01

    Oxidative stress and oxidative photodamage induced by UV radiation can cause serious skin damage that is characterized by wrinkling, roughness, laxity and pigmentation. The effects of a sea buckthorn (Hippophae rhamnoides L.) fruit blend (SFB) containing sea buckthorn fruit extract, blueberry extract and collagen on UV-induced skin aging were examined by treating hairless mice for 6 weeks with UV irradiation and SFB administered orally. The effects of SFB were measured in the skin of these mice by phenotypical and histological analysis and western blotting. According to wrinkle formation analysis, the oral intake of SFB induced a decrease in wrinkle formation in the damaged skin of UV-irradiated mice. The thickness of the epidermis and dermis in the vitamin extracts (Vit)- and SFB-treated group was lower than that in the vehicle-treated group, but the group treated with SFB50 was the most effective group. The mice treated with the Vit- or SFB solution maintained a normal moisture content through the inhibition of transdermal water loss (TEWL) and an increase in skin moisture content. Furthermore, the levels of matrix metalloproteinase (MMP) and collagen protein expression were assessed in five groups to examine the mechanisms underlying the effects of SFB oral intake. The application of SFB induced a decrease in MMP-1 and -9 expression to the levels observed in the vehicle-treated group, but MMP-9 expression showed a much larger decrease than MMP-1. Furthermore, the expression of collagen-1 in the skin corresponded to MMP expression except for the SFB30-treated group, whereas the superoxide dismutase (SOD) activity was increased dramatically in the SFB50-treated group. These results suggest that SFB has potential as a protective and therapeutic drug candidate against skin aging that functions by regulating the moisture content, MMP expression levels and SOD activity.

  13. Use of electron beam irradiation to improve the microbiological safety of Hippophae rhamnoides

    Energy Technology Data Exchange (ETDEWEB)

    Minea, R. [National Institute for Lasers, Plasma and Radiation Physics, Electron Accelerators Department, 409 Atomistilor St., Bucharest-Magurele 077125 (Romania); Nemtanu, M.R. [National Institute for Lasers, Plasma and Radiation Physics, Electron Accelerators Department, 409 Atomistilor St., Bucharest-Magurele 077125 (Romania)], E-mail: monica.nemtanu@inflpr.ro; Manea, S.; Mazilu, E. [S.C. Hofigal Export-Import S.A., 2A Intrarea Serelor St., 75669, Bucharest 4 (Romania)

    2007-09-21

    Sea buckthorn (Hippophae rhamnoides) is increasingly used in food supplements due to its dietary and medicinal compounds with a beneficial role in human diet and health. As many other medicinal plants, sea buckthorn can be contaminated with microorganisms which exerts an important impact on the overall quality of the products. Irradiation is an effective method for food preservation because it is able to destroy pathogenic microorganisms keeping the organoleptic and nutritional characteristics of the foods. The objective of the present study was to investigate the application of electron beam irradiation in order to improve the microbiological safety of sea buckthorn. The experimental results indicated that the electron beam treatment might be a good method to remove undesirable microorganisms from sea buckthorn without significant changes in its active principles.

  14. UHPLC/PDA–ESI/MS Analysis of the Main Berry and Leaf Flavonol Glycosides from Different Carpathian Hippophaë rhamnoides L. Varieties

    NARCIS (Netherlands)

    Pop, R.M.; Socaciu, C.; Pintea, A.; Buzoianu, A.D.; Sanders, M.G.; Gruppen, H.; Vincken, J.P.

    2013-01-01

    Introduction - Sea buckthorn (Hippophaë rhamnoides L.) is known to be rich in many bioactive compounds (such as vitamins, phenolics, carotenoids) important for human health and nutrition. Among the phenolics, berries and leaves contain a wide range of flavonols that are good quality and authenticity

  15. Flavonol glycosides of sea buckthorn (Hippophaë rhamnoides ssp. sinensis) and lingonberry (Vaccinium vitis-idaea) are bioavailable in humans and monoglucuronidated for excretion.

    Science.gov (United States)

    Lehtonen, Henna-Maria; Lehtinen, Outi; Suomela, Jukka-Pekka; Viitanen, Matti; Kallio, Heikki

    2010-01-13

    Glucuronidation and excretion of sea buckthorn and lingonberry flavonols were investigated in a postprandial trial by analyzing the intact forms of flavonol glycosides as well as glucuronides in plasma, urine, and feces. Four study subjects consumed sea buckthorn (study day 1) and lingonberry (study day 2) breakfasts, and blood, urine, and fecal samples were collected for 8, 24, and 48 h, respectively. Both glycosides and glucuronides of the flavonol quercetin as well as kaempferol glucuronides were detected in urine and plasma samples after the consumption of lingonberries; 14% of flavonols in urine were glycosides, and 86% were glucuronidated forms (wt %). After the consumption of sea buckthorn, 5% of flavonols excreted in urine were detected intact, and 95% as the glucuronides (wt %). Solely glucuronides of flavonols isorhamnetin and quercetin were found in plasma after the consumption of sea buckthorn berries. Only glycosides were detected in the feces after each berry trial. Flavonol glycosides and glucuronides remained in blood and urine quite long, and the peak concentrations appeared slightly later than previously described. The berries seemed to serve as a good flavonol supply, providing steady flavonol input for the body for a relatively long time.

  16. Genome-wide analysis of long non-coding RNAs at the mature stage of sea buckthorn (Hippophae rhamnoides Linn) fruit.

    Science.gov (United States)

    Zhang, Guoyun; Duan, Aiguo; Zhang, Jianguo; He, Caiyun

    2017-01-05

    Long non-coding RNAs (lncRNAs), which are >200nt longer transcripts, potentially play important roles in almost all biological processes in plants and mammals. However, the functions and profiles of lncRNAs in fruit is less understood. Therefore, it is urgent and necessary to identify and analyze the functions of lncRNAs in sea buckthorns. Using RNA-sequencing, we synthetically identified lncRNAs in mature fruit from the red and yellow sea buckthorn. We obtained 567,778,938 clean reads from six samples and identified 3428 lncRNAs in mature fruit, including 2498 intergenic lncRNAs, 593 anti-sense lncRNAs, and 337 intronic lncRNAs. We also identified 3819 and 2295 circular RNAs in red and yellow sea buckthorn Fruit. In the aspects of gene architecture and expression, our results showed significant differences among the three lncRNA subtypes. We also investigated the effect of lncRNAs on its cis and trans target genes. Based on target genes analysis, we obtained 61 different expression lncRNAs (DE-lncRNAs) between these two sea buckthorns, including 23 special expression lncRNAs in red fruit and 22 special expression lncRNAs in yellow fruit. Importantly, we found a few DE-lncRNAs play cis and trans roles for genes in the Carotenoid biosynthesis, ascorbate and aldarate metabolism and fatty acid metabolism pathways. Our study provides a resource for lncRNA studies in mature fruit. It probably encourages researchers to deeply study fruit-coloring. It expands our knowledge about lncRNA biology and the annotation of the sea buckthorn genome. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Postprandial hyperglycemia and insulin response are affected by sea buckthorn (Hippophaë rhamnoides ssp. turkestanica) berry and its ethanol-soluble metabolites.

    Science.gov (United States)

    Lehtonen, H-M; Järvinen, R; Linderborg, K; Viitanen, M; Venojärvi, M; Alanko, H; Kallio, H

    2010-12-01

    Repeated postprandial hyperglycemia and subsequent mild, late hypoglycemia as well as high postprandial insulin response lead to metabolic events that may eventually develop into type 2 diabetes. The aim of this study was to assess how sea buckthorn berries as well as two sea buckthorn extraction residues modulate the postprandial metabolism after a high-glucose meal. Ten healthy normal-weight male volunteers consumed four study breakfasts, one control (A) and three sea buckthorn meals on four distinct study days. All the meals contained yoghurt and glucose (50 g). The sea buckthorn ingredients used were dried and crushed whole berries (meal B1), supercritical fluid (SF)-carbon dioxide (CO(2))-extracted oil-free berries (meal B2) or ethanol-extracted SF-CO(2)-extraction residue (meal B3). Blood samples for glucose, insulin and tumor necrosis factor-α analyses were collected before and during the 6-h study period. Meal B1 suppressed the postprandial peak insulin response when compared with meal A (Δconcentration of 30-min peak value--21.8 mU/l, P=0.039), and stabilized postprandial hyperglycemia and subsequent hypoglycemia (Δconcentration of 30-min peak value--120-min value -30.4 mU/l, P=0.036). Furthermore, meal B2 resulted in a more stable insulin response than the control meal (Δconcentration of 30-min peak value--120-min value -25.9 mU/l, P=0.037). Removal of the CO(2)-soluble oil component from the berries did not show a significant change in the studied postprandial effects of the berries. The EtOH soluble components, again showed advantageous properties in both insulin and glucose responses.

  18. Phytochemicals content and antioxidant properties of sea buckthorn (Hippophae rhamnoides L.) as affected by heat treatment - Quantitative spectroscopic and kinetic approaches.

    Science.gov (United States)

    Ursache, Florentina-Mihaela; Ghinea, Ioana Otilia; Turturică, Mihaela; Aprodu, Iuliana; Râpeanu, Gabriela; Stănciuc, Nicoleta

    2017-10-15

    The effect of thermal processing (50-100°C) on the degradation of the phytochemicals in sea buckthorn extract was investigated using chromatographic, fluorescence and FT-IR spectroscopy techniques and degradation kinetics. Heating the sea buckthorn extract resulted in structural changes that led to red- or blue-shifts in maximum emission, depending on temperature and excitation wavelengths. The attenuated total reflectance analysis of the sea buckthorn extract revealed a satisfactory thermostability of compounds at high temperatures. A fractional conversion kinetic model was used to describe the mechanism of degradation in terms of rate and activation energy. Activation energies for total carotenoids, polyphenolic, flavonoids, and antioxidant activity were 8.45±0.93kJ/mol, 2.50±0.66kJ/mol, 22.50±7.26kJ/mol and 15.22±2.75kJ/mol, respectively. The kinetic parameters evidence a higher thermal stability of carotenoids and polyphenols, suggesting higher degradation rates for flavonoids and antioxidant activity. Our results demonstrate that industrial process optimization in terms of time-temperature combinations demands product specific kinetic data. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Significance of Hippophae rhamnoides L. in evolution of the Eemian Interglacial' flora in Warsaw area

    Directory of Open Access Journals (Sweden)

    Krzysztof M. Krupiński

    2014-01-01

    Full Text Available Palynologic studies of sediments from Warsaw-Wawrzyszew and other sites in the Warsaw Basin enabled to analyze main occurrence phases of Hippophae rhamnoides during the widely understood Lake Pleistocene interglacial warming. In initial phases of the Eemian Interglacial (late glacial of a preceding glaciation the area of Warsaw-Wawrzyszew indicated especially favourable conditions for such rich development of sea-buckthorn. Analysis of occurrence phases of this bush proves that besides climatic conditions, great influence on more abundant occurrence of sea-buckthorn in its late glacial (pre-interglacial part could be played by edaphic conditions, mainly contents of basic compounds (CaCO3 in a substrate.

  20. Phenolic compounds, antioxidant activity, antiproliferative activity and bioaccessibility of Sea buckthorn (Hippophaë rhamnoides L.) berries as affected by in vitro digestion.

    Science.gov (United States)

    Guo, Ruixue; Chang, Xiaoxiao; Guo, Xinbo; Brennan, Charles Stephen; Li, Tong; Fu, Xiong; Liu, Rui Hai

    2017-11-15

    Phenolics, antioxidant and antiproliferative properties of Sea buckthorn berries were evaluated using a simulated in vitro digestion and compared with a chemical extraction method. Digested samples were subjected to antiproliferation evaluation against human liver, breast and colon cancer cells. Furthermore, the bioaccessibility of digested berries was evaluated using a Caco-2 cell culture model. Results revealed that after enzymatic digestion the phenolic compounds were quite different from the chemical extracts, more flavonoid aglycones were released, whereas less total phenolics, phenolic acids and flavonoid glycosides were detected. Although the extracellular antioxidant activity of the digesta was lower than that of extracts, the cellular antioxidant activity (CAA) and antiproliferative effects of berries were significantly enhanced by digestion. This was attributed to their higher flavonoid aglycone content and could be verified by testing individual active compounds, suggesting that the cellular uptake of samples might be improved, which was also certified by the Caco-2 cell uptake model. The digested samples showed an almost 5-fold cellular accumulative amount of isorhamnetin than pure isorhamnetin, which was attributed to the significant down regulation of the mRNA expression level of efflux transporters MRP2 and P-gp. This finding indicated that the digestion enhanced the bioaccessibility of bioactive compounds of berries.

  1. Sea Buckthorn (Hippophaë rhamnoides L.) Oil Improves Atopic Dermatitis-Like Skin Lesions via Inhibition of NF-κB and STAT1 Activation.

    Science.gov (United States)

    Hou, Dian-Dong; Di, Zheng-Hong; Qi, Rui-Qun; Wang, He-Xiao; Zheng, Song; Hong, Yu-Xiao; Guo, Hao; Chen, Hong-Duo; Gao, Xing-Hua

    2017-01-01

    The objective of this study was to evaluate the topical effects of sea buckthorn (SBT) oil on atopic dermatitis (AD)-like lesions in a mouse model generated by repeated topical administration of DNCB in BALB/c mice. DNCB was applied repeatedly on the dorsal skin of mice to induce AD-like lesions. Following AD induction, SBT oil was applied daily on the dorsal skin for 4 weeks. The severity of skin lesions was examined macroscopically and histologically. We further measured the production of MDC/CCL22 and TARC/CCL17 in IFN-γ/TNF-α activated HaCaT cells. Topically applied SBT oil in DNCB-treated mice ameliorated the severity score of dermatitis, decreased epidermal thickness, reduced spleen and lymph node weights, and prevented mast cell infiltration. In addition, SBT oil suppressed the Th2 chemokines TARC and MDC via dose-dependent inhibition of NF-κB, JAK2/STAT1, and p38-MAPK signaling pathways in IFN-γ/TNF-α-activated HaCaT cells. These results suggest that SBT oil had a beneficial effect on AD-like skin lesions, partially via inhibition of the Th2 chemokines TARC and MDC in inflamed skin. © 2017 S. Karger AG, Basel.

  2. Evaluation of chemical constitute, fatty acids and antioxidant activity of the fruit and seed of sea buckthorn (Hippophae rhamnoides L.) grown wild in Iran.

    Science.gov (United States)

    Saeidi, Keramatollah; Alirezalu, Abolfazl; Akbari, Zahra

    2016-01-01

    In this investigation, the chemical compositions of berries from sea buckthorn were studied. The amount of ascorbic acid and β-carotene determined by HPLC was 170 mg/100 g FW and 0.20 mg/g FW, respectively. Total phenols, anthocyanins, acidity and total soluble solids (TSS) contents were 247 mg GAE/100 g FW, 3 mg/L (cyanidin-3-glucoside), 5.32% and 13.8%, respectively. Fruit antioxidant activity determined by the ferric reducing ability of plasma (FRAP) method was 24.85 mM Fe/100 g FW. Results confirmed the presence of six dominant fatty acids (determined by GC) in fruit including linoleic (34.2%), palmitoleic (21.37%), palmitic (17.2%), oleic (12.8%), linolenic (5.37%) and stearic acid (1.67%). Five dominant fatty acids of the seeds were linoleic (42.36%), linolenic (21.27%), oleic (21.34%), palmitic (6.54%) and stearic acid (2.54%). The nitrogen content was 3.96%. The P, K, Ca, Mg, Fe, Zn, Mn, Cu, Cd and Cl contents of fruit were 491, 1674, 1290, 990, 291, 29.77, 108.37, 17.87, 0.021 and 2.18 mg/kg DW, respectively.

  3. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

    Directory of Open Access Journals (Sweden)

    Javid Ali

    2015-04-01

    Full Text Available Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane of Hippophae rhamnoides (H. rhamnoides leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution were transferred to specific concentration of each extract. Results: The mortality of aqueous extract was 46.7%, methanol extract was 46.7%, ethanolic extract was 50.0%, ethyl acetate was 26.7%, acetone extract was 33.3%, chloroform extract was 40.0% and n-hexane extract was 33.3%. The lowest LD50 was found in methanol extracts (1199.97 µg/mL. Brine shrimp cytotoxicity of tested extracts of H. rhamnoides showed that mortality rate was concentration dependent. Conclusions: It is concluded that bioactive components are present in all leaves extracts of H. rhamnoides, which could be accounted for its pharmacological effects. Thus, the results support the uses of this plant species in traditional medicine.

  4. [Community characteristics of Hippophae rhamnoides forest and water and nutrient condition of the woodland in Loess hilly region].

    Science.gov (United States)

    Ruan, Chengjiang; Li, Daiqiong

    2002-09-01

    To improve the productivity of Hippophae rhamnoides forest and to manage the forest reasonably, the community characteristics of Hippophae rhamnoides and the water and nutrient condition of the woodland were analyzed. The results showed that Hippophae rhamnoides grew fast and its adaptability was strong. 4-5 years old Hippophae rhamnoides could form shrubs-grass community. The biomass 1-8 years old Hippophae rhamnoides increased rapidly, but that of 8-11 years old increased more slowly. Hippophae rhamnoides older than 11 years could keep reasonable community structure and higher primary productivity by natural thinning. The utilization of soil moisture by Hippophae rhamnoides could be classified into four layers as faint utilization layer of root system (0-20 cm), utilization of root system (20-300 cm), adjustment layer supplied by soil moisture (300-400 cm), and faint adjustment layer (400-500 cm). Due to the soil improvement by Hippophae rhamnoides and the water retaining by litter, the soil moisture of 1-1.5 m layer could be easy to restore. Hippophae rhamnoides could keep the balance of nutrition in the woodland through the fixation of nitrogen by root nodule and the decomposition of litter. In the 6-9 years old Hippophae rhamnoides plantation, the total nitrogen content of soil might be improved from 0.05-0.1% to 0.2%.

  5. Associations of SRAP markers with dried-shrink disease resistance in a germplasm collection of sea buckthorn (Hippophae L.).

    Science.gov (United States)

    Li, He; Ruan, Cheng-Jiang; Teixeira da Silva, Jaime A; Liu, Bao-Quan

    2010-06-01

    Sea buckthorn (Hippophae L.) is a woody, outcrossing dioecious pioneer plant, being widely planted as a new berry crop with rich nutritional and medicinal compounds. This long-juvenile and long-lived woody plant can be more difficult to cultivate than other crop plants. Dried-shrink disease (DSD) is a dangerous pathogen that destroys sea buckthorn and halts commercial production. We estimated variability of sequence-related amplified polymorphism (SRAP) markers in 77 accessions of 22 sea buckthorn cultivars to seek markers associated with DSD resistance and help to identify potential breeding cultivars. Seventeen SRAP primer combinations generated 289 bands, with a mean of 17 bands per primer combination. At a Dice coefficient of 0.852, the dendrogram generated with 191 polymorphic bands clustered 73 accessions of Hippophae rhamnoides into 2 groups and 4 accessions of Hippophae salicifolia into 1 group. Eleven SRAP markers (Me1-Em3(600), Me1-Em1(680), Me2-Em1(650), Me2-Em1(950), Me3-Em6(1300), Me2-Em6(320), Me2-Em6(400), Me1-Em2(600), Me1-Em1(1200), Me1-Em1(1700), Me2-Em2(250)) were significantly correlated with DSD resistance (P < 0.001). These markers provide a viable option for breeding programs that select lineages with DSD resistance, especially when no other genetic information, such as linkage maps and quantitative trait loci, are available.

  6. Anti-Aging Effects of Hippophae rhamnoides Emulsion on Human Skin

    African Journals Online (AJOL)

    Purpose: This study aimed to evaluate the effects of topically applied water-in-oil (w/o) emulsion of Hippophae rhamnoides using standard R cutometer parameters. Methods: A w/o emulsion of 1 % hydro-alcoholic extract of H. rhamnoides (formulation) and placebo control (base) were used in the study. Eleven healthy male ...

  7. Biochemical characterization of sea buckthorn ( Hippophae ...

    African Journals Online (AJOL)

    Sea buckthorn is a valuable medicinal plant, cultivated and naturally grown in northern Pakistan. The plant produces berry with small hard seed in the centre. The seed is the source of all nutrients and phytochemcials. However, there is lack of literature regarding the biochemical and physico-chemical quality of the seed.

  8. A new hydrocarbon material based on seabuckthorn ( Hippophae rhamnoides) sawdust: A structural promoter of cobalt catalyst for Fischer-Tropsch synthesis

    Science.gov (United States)

    Pankina, G. V.; Chernavskii, P. A.; Lunin, V. V.

    2016-09-01

    Aspects of the physicochemical properties of a hydrocarbon material based on seabuckthorn ( Hippophae rhamnoides) sawdust are studied. The use of a hydrocarbon material based on sea buckthorn sawdust as a structural promoter of Co/CHip cobalt catalyst in the reaction of CO hydrogenation is shown to require an additional cycling stage in the mode of reduction and oxidation. The resulting mean size of the Co particles is found to be 18-19 nm and is considered acceptable for the synthesis of C5+ liquid hydrocarbons.

  9. The Protective Effect of Hippophae Rhamnoides Carotenoid Extract Against Lipid Peroxidation in Crude Vegetable Oils

    Directory of Open Access Journals (Sweden)

    Sanda Andrei

    2014-11-01

    Full Text Available Vegetable oils are important elements of the human diet because they contain essential nutritional factors. Due to the manufacturing processes or inadequate conditions of storage, they may also contain lipid oxidation products that are toxic to the body. The purpose of this paper is to test the protective effect of carotenoid-rich extracts obtained from the fruits of Hippophae rhamnoides on crude sunflower, pumpkin and olive oils oxidative processes. In order to evaluate the effect of antioxidant carotenoids, three stages were followed: thermal induction of lipid peroxidation in the presence of AAPH (2,2'-Azobis(2-amidinopropane dihydrochloride; determination of the level of lipid peroxidation in oxidized oils in the presence and absence of antioxidants, by quantifying the concentration of conjugated dienes and malonyl dialdehyde (MDA; determination of the level of lipid peroxidation by evaluating the profile of the fatty acids and the ratio between the saturated and unsaturated fatty acids (UFA / SFA, using an GC-MS method. In the case of sunflower oil, it was observed that sea buckthorn fruit extract significantly decreased MDA concentration but does not significantly reduce the concentration of conjugated dienes. The protective effect of carotenoids is more evident in the case of oil from pumpkin seeds. In the olive oil, unlike the first two types of oils, the carotenoids extract inhibits both the MDA and the conjugated dienes formation to a lesser extent, statistically insignificant. Overall, the ratio UFA / SFA decreases in crude oxidized oils. In the oils in which carotenoids were added was observed an increase in the UFA / SFA ratio. Carotenoids fraction from sea buckthorn fruits, rich in xanthophylls’ esters, possess a good antioxidant effect, protecting vegetable oils against peroxidation processes induced in the presence of AAPH

  10. Effect of Sea-Buckthorn (Hippophaë rhamnoides L.) Pulp Oil Consumption on Fatty Acids and Vitamin A and E Accumulation in Adipose Tissue and Liver of Rats.

    Science.gov (United States)

    Czaplicki, Sylwester; Ogrodowska, Dorota; Zadernowski, Ryszard; Konopka, Iwona

    2017-06-01

    An in vivo experiment was conducted to determine the effect of sea-buckthorn pulp oil feeding on the fatty acid composition of liver and adipose tissue of Wistar rats and the liver accumulation of retinol, its esters and α-tocopherol. For a period of 28 days, rats were given a modified casein diet (AIN-93) in which sea-buckthorn pulp oil, soybean oil and pork lard were used as sources of fat. Compared to the other fat sources, sea-buckthorn pulp oil was the most abundant in C16 fatty acids, carotenoids (mainly β-carotene) and tocopherols (mainly α-tocopherol). Its consumption was reflected in an increased share of palmitoleic acid in adipose tissue and the liver and an increased level of retinol in liver tissues (this was not observed for its esters). Although the type of fat did not have a significant effect on the average content of α-tocopherol in the liver, the variation of saturation of this tissue with α-tocopherol was the lowest when rats were fed a diet containing sea-buckthorn oil. This experiment indicates the possibility of affecting adipose tissue and liver by a diet.

  11. The Evaluation of Synergistic Effect of Hippophae rhamnoides and Vitamin E on Growth Performance and Oxidative Stress at Oreochromis niloticus - Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Alina Antache

    2014-10-01

    Full Text Available The aim of this research is to evaluate the influence of sea buckthorn (Hippophae rhamnoides and vitamin E on growth performance indicators and oxidative stress at Nile tilapia juvenile, reared in a recirculating aquaculture system. The experiment was conducted six weeks, in triplicate. The experimental variants were: V1 – control, V2 – 1% sea buckthorn / kg feed, V3 – 500mg vitamin E / kg feed and V4 – 1% sea buckthorn supplemented with 500 mg vitamin E / kg feed. During the experiment was performed an intermediary biometric measurement. Oxidative stress analysis consisted in determination of lipid peroxidation (MDA-malondialdehide and total antioxidant capacity (TAC from liver, tissue and gut. Results showed a good evolution of GR, FCR and SGR, during the experiment, in V4 – in which feed was supplemented with sea buckthorn and vitamin E. Based on the results obtained in variant V4, in liver and tissue, the oxidative stress was reduced. Regarding MDA and TAC, between experimental variants, were registered significant differences (p<0.05 at the level of tissue and gut. In conclusion, the research shows that sea buckthorn (1%/kg feed in combination with Vitamin E (500mg/kg feed has a synergistic effect on growth performance indicators and oxidative stress, at Oreochromis niloticus juvenile.

  12. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns

    Directory of Open Access Journals (Sweden)

    Nitin K. Upadhyay

    2011-01-01

    Full Text Available The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae (SBT and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III. The histological examinations and matrix metalloproteinases (MMP-2 and -9 expression also confirmed the healing efficacy of SBT leaf extract. Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue. The SBT also promoted angiogenesis as evidenced by an in vitro chick chorioallantoic membrane model and in vivo up-regulated vascular endothelial growth factor (VEGF expression. The SBT leaf extract had no cytotoxic effect on BHK-21 cell line. In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.

  13. Consumer-led development of novel sea-buckthorn based beverages

    DEFF Research Database (Denmark)

    Geertsen, Julie Leth; Allesen-Holm, Bodil Helene; Giacalone, Davide

    2016-01-01

    In the context of a growing interest toward foods of local origin, this research explored consumer perceptions of novel sea-buckthorn-based beverages (SBBs), developed by combining sea-buckthorn (hippophae rhamnoides L.) berries in combination with other locally sourced ingredients. Specifically...

  14. Growth and physiological responses to drought and elevated ultraviolet-B in two contrasting populations of Hippophae rhamnoides

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    Yongqing Yang; Yinan Yao; Gang Xu [Chinese Academy of Sciences, Chengdu Inst. of Biology, Chengdu (China); Chunyang Li [Graduate School of the Chinese Academy of Sciences, Beijing (China)

    2005-08-01

    In the southeast of the Qinghai-Tibetan Plateau of China, sea buckthorn (Hippophae rhamnoides L.), which is a thorny nitrogen-fixing deciduously perennial shrub, has been widely used in forest restoration as the pioneer species. In our study, two contrasting populations from the low and high altitudinal regions were employed to investigate the effects of drought, ultraviolet-B (UV-B) and their combination on sea buckthorn. The experimental design included two watering regimes (well watered and drought stressed) and two levels of UV-B (with and without UV-B supplementation). Drought significantly decreased total biomass, total leaf area and specific leaf area (SLA), and increased root/shoot ratio, fine root/coarse root ratio and abscisic acid content (ABA) in both populations. However, the high altitudinal population was more responsive to drought than the low altitudinal population. On the other hand, elevated UV-B induced increase in anthocyanins in both populations, whereas he accumulation of UV-absorbing compounds occurred only in the low altitudinal population. The drought-induced enhancement of ABA in the high altitudinal population was significantly suppressed in the combination of drought and elevated UV-B. Moreover, significant drought x UV-B interaction was detected on total biomass in both populations, total leaf area and fine root/coarse root in the low altitudinal population, and SLA in the high altitudinal population. These results demonstrated that there were different adaptive responses between two contrasting populations, the high altitudinal population exhibited higher tolerance to drought and UV-B than the low altitudinal population. (au)

  15. Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma

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    Beata Olas

    2016-01-01

    Full Text Available Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation and the generation of superoxide anion (O2-∙ in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2 or H2O2/Fe (a donor of hydroxyl radicals. The tested fraction of H. rhamnoides (0.5– 50 µg/mL; the incubation time: 15 and 60 min inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2-∙ in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL. The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

  16. Metabolic discrimination of sea buckthorn from different Hippophaë species by1H NMR based metabolomics.

    Science.gov (United States)

    Liu, Yue; Fan, Gang; Zhang, Jing; Zhang, Yi; Li, Jingjian; Xiong, Chao; Zhang, Qi; Li, Xiaodong; Lai, Xianrong

    2017-05-08

    Sea buckthorn (Hippophaë; Elaeagnaceae) berries are widely consumed in traditional folk medicines, nutraceuticals, and as a source of food. The growing demand of sea buckthorn berries and morphological similarity of Hippophaë species leads to confusions, which might cause misidentification of plants used in natural products. Detailed information and comparison of the complete set of metabolites of different Hippophaë species are critical for their objective identification and quality control. Herein, the variation among seven species and seven subspecies of Hippophaë was studied using proton nuclear magnetic resonance ( 1 H NMR) metabolomics combined with multivariate data analysis, and the important metabolites were quantified by quantitative 1 H NMR (qNMR) method. The results showed that different Hippophaë species can be clearly discriminated and the important interspecific discriminators, including organic acids, L-quebrachitol, and carbohydrates were identified. Statistical differences were found among most of the Hippophaë species and subspecies at the content levels of the aforementioned interspecific discriminators via qNMR and one-way analysis of variance (ANOVA) test. These findings demonstrated that 1 H NMR-based metabolomics is an applicable and effective approach for simultaneous metabolic profiling, species differentiation and quality assessment.

  17. Effect of modified atmosphere packaging (MAP) on quality of Sea Buckthorn during postharvest storage

    Science.gov (United States)

    Modified atmosphere packaging (MAP) has been used to retain the quality of postharvest produce. In the present study the effect of MAP on quality of berry fruit of Sea buckthorn (Hippophae rhamnoides L., a hardy, deciduous shrub, native to Asia) during refrigerated storage was investigated. Sea buck...

  18. The beneficial health aspects of sea buckthorn (Elaeagnus rhamnoides (L.) A.Nelson) oil.

    Science.gov (United States)

    Olas, Beata

    2018-03-01

    Plant oils are known to have biological activity. This review paper summarizes the current knowledge of the composition of sea buckthorn (Elaeagnus rhamnoides (L.) A.Nelson) seed and pulp oil and its beneficial health aspects. In vitro and in vivo studies on humans and animals have found sea buckthorn oil to have a variety of beneficial properties to human health, and indicate that it may be a valuable component of human and animal nutrition. Various bioactive substances are present in all parts of sea buckthorn, and these are used traditionally as raw material for health foods and as nutritional supplements. The oil, berries, leaves and bark have medicinal properties, and the fruits have a unique taste; these parts can be processed to make oil, juice, jam, jellies and candies, as well as alcoholic and non-alcoholic beverages. Sea buckthorn oil may be extracted from the seed or the pulp. The mature seeds contain 8-20% oil and the dried fruit pulp about 20-25%, while the fruit residue contains about 15-20% oil after juice extraction. These oils have high concentrations of lipophilic constituents, most commonly unsaturated fatty acids (UFAs), phytosterols and vitamins A and E. These components have a multifunctional effect on human health, with the fatty acids playing an important function in modifying cerebrovascular and cardiovascular disorders. The oil also has anti-oxidant, anti-inflammatory and anti-depressive properties. Sea buckthorn is a unique plant. Its beneficial properties against cardiovascular disorders have been attributed to its high UFA content and range of phytosterols, especially beta-sitosterol. However, its different action on the human organism remain unclear, and further well-controlled, high-quality experiments with human subjects are required to determine the prophylactic and therapeutic doses of sea buckthorn oil for use in clinical studies. Additional studies are also needed to understand the action by which the oil exerts its beneficial

  19. MINING THE PROTEIN REPERTOIRE OF A HIMALAYAN SHRUB, HIPPOPHAE RHAMNOIDES FOR ANTIFREEZE PROTEINS

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    Bhavana Sharma

    2016-09-01

    Full Text Available Seabuckthorn (Hippophae rhamnoides, a cold desert shrub, can survive freezing temperature conditions and is considered as frost and drought tolerant. Earlier, antifreeze activity was reported in seabuckthorn seedlings grown under laboratory conditions. No reports are available on the cold hardiness of this huge bioresource available naturally in the Himalayan region. Detection of antifreeze activity in leaves and berries (splat assay and nanoliter osmometer confirmed the presence of putative antifreeze proteins (AFPs which may help in the survival of this plant under freezing conditions. Flower shaped ice crystals were observed in both leaves and berries while hexagonal ice crystals in seedlings indicated comparatively higher antifreeze activity in the wild parts. Splat assay results confirmed highest IRI activity in leaf (a 2.75 fold decrease in mean grain size of ice crystal after annealing followed by berry (with 1.75 fold decrease and least in the seedlings (with 1.5 fold decrease. Gel filtration chromatography resolved leaf fractions exhibited antifreeze activity in 34, 36 and 41 kDa while in berry fractions a 41 kDa polypeptide showed antifreeze activity. This is the first report showing presence of AFPs in (H. rhamnoides leaf and berry. Shotgun proteomic analysis using Q-Exactive Orbitrap High Resolution Mass Spectrometer and functional annotation of leaf and berry proteins revealed their association with primary, secondary metabolism, defence/stress, redox regulation, signalling and structural remodelling majority of which are affected during cold stress. Further purification of these AFPs could open gates for commercial utilization of this plant growing abundantly in Himalayan regions of India.

  20. Modulation of Hypoxia-Induced Pulmonary Vascular Leakage in Rats by Seabuckthorn (Hippophae rhamnoides L.

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    Jayamurthy Purushothaman

    2011-01-01

    Full Text Available Cerebral and pulmonary syndromes may develop in unacclimatized individuals shortly after ascent to high altitude resulting in high altitude illness, which may occur due to extravasation of fluid from intra to extravascular space in the brain, lungs and peripheral tissues. The objective of the present study was to evaluate the potential of seabuckthorn (SBT (Hippophae rhamnoides L. leaf extract (LE in curtailing hypoxia-induced transvascular permeability in the lungs by measuring lung water content, leakage of fluorescein dye into the lungs and further confirmation by quantitation of albumin and protein in the bronchoalveolar lavage fluid (BALF. Exposure of rats to hypoxia caused a significant increase in the transvascular leakage in the lungs. The SBT LE treated animals showed a significant decrease in hypoxia-induced vascular permeability evidenced by decreased water content and fluorescein leakage in the lungs and decreased albumin and protein content in the BALF. The SBT extract was also able to significantly attenuate hypoxia-induced increase in the levels of proinflammatory cytokines and decrease hypoxia-induced oxidative stress by stabilizing the levels of reduced glutathione and antioxidant enzymes. Pretreatment of the extract also resulted in a significant decrease in the circulatory catecholamines and significant increase in the vasorelaxation of the pulmonary arterial rings as compared with the controls. Further, the extract significantly attenuated hypoxia-induced increase in the VEGF levels in the plasma, BALF (ELISA and lungs (immunohistochemistry. These observations suggest that SBT LE is able to provide significant protection against hypoxia-induced pulmonary vascular leakage.

  1. Efficacy of aqueous extract of Hippophae rhamnoides and its bio-active flavonoids against hypoxia-induced cell death.

    Science.gov (United States)

    Tulsawani, Rajkumar; Gupta, Rashmi; Misra, Kshipra

    2013-01-01

    To investigate the protective efficacy of aqueous extract of Hippophae rhamnoides against chronic hypoxic injury using primary rat hepatocytes. The extract was prepared using maceration method and characterized by its phenolic and flavonoid content and chemical antioxidant capacity using ferric reducing antioxidant power assay. Hepatocytes were maintained in hypoxia chamber (3% and 1% oxygen) for 72 h. The cells kept under normoxic condition served as control. The cells were treated with the extract and flavonoids; isorhamentin, kaempferol or qurecetin-3-galactoside. After the end of exposure period; cell survival, reactive oxygen species (ROS), leakage of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), reduced glutathione (GSH), glutathione peroxidase (GPx), and superoxide dismutase (SOD) levels were measured. The extract showed presence of high phenolic and flavonoid content with significant antioxidant activity in chemical assay. The cell exposed to hypoxia showed concentration dependent cell death and harbored higher reactive oxygen species. In addition, these cells showed significant leakage of intracellular LDH, ALT, and AST accompanied by the diminished levels/activities of GSH, GPx, and SOD. The treatment of cells with aqueous extract of H. rhamnoides reduced hypoxia-induced cell death and prevented increase in ROS levels and leakage of intracellular LDH, ALT, and AST from cells. Moreover, these cells maintained better levels/activities of GSH, GPx, and SOD in comparison to the respective controls. The major flavonoids present in aqueous extract of H. rhamnoides; quercetin-3-galactoside, kaempferol, and isorhamentin also prevented hypoxia induced cell injury individually or in combination, however, the protection offered by these compounds taken together could not match to that of the extract. Overall the findings reveal significance of aqueous extract of H. rhamnoides in controlling ROS-meditated hypoxic

  2. Factors affecting growth and nodulation of Hippophae rhamnoides L. ssp. Rhamnoides in soils from two successional stages of dune formation

    Energy Technology Data Exchange (ETDEWEB)

    Oremus, P.A.I.; Otten, H. (Department of Dune Research Weevers' Diun, Oostvoorne (Netherlands). Inst. for Ecological Research)

    1981-01-01

    To explain the decline of Hippophae scrub in the vegetation succession in the dunes of The Netherlands the growth and nodulation of Hippophae plants grown in pots, using soil from an early stage (site AH) and a post-optimum stage (site HP), were investigated. In HP-soil nodulation, yield, and the nitrogen and phosphorus content of test plants were always lower and the number of necrotic nodules and the dry matter content were always higher than in AH-soil, even after inoculation with crushed nodules and the addition of a nutrient solution. Plants in HP-soil also had darker roots, less root hairs, a higher number of short lateral roots and a higher percentage of dead roots than those in AH-soil. These characteristics of adverse growth conditions disappeared upon ignition or gamma irradiation of HP-soil. Possible explanations of these results are discussed.

  3. THE ISOLATION, ANALISIS AND TESTING OF SEA BUCKTHORN CAROTENOIDS ON MURINE MACROPHAGE CULTURES

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    MICLEA ILEANA

    2007-01-01

    Full Text Available Our aim was to characterize a sea buckthorn (Hippophae rhamnoides fruitcarotenoidic extract and evaluate its influence on macrophage phagocytic responsegiven that carotenoids function as antioxidants. Carotenoids were isolated,saponified and analysed using HPLC before and after saponification. Murineperitoneal macrophages were cultured in medium containing carotenoidic extract.Carotenoid influence on phagocytosis was assessed by calculating the phagocyticindex (PI % and mean phagocytosis (MP. The positive significant differences(p<0,05 picture carotenoid ability to enhance cell phagocytic activity.

  4. THE ISOLATION, ANALISIS AND TESTING OF SEA BUCKTHORN CAROTENOIDS ON MURINE MACROPHAGE CULTURES

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    ILEANA MICLEA

    2007-05-01

    Full Text Available Our aim was to characterize a sea buckthorn (Hippophae rhamnoides fruitcarotenoidic extract and evaluate its influence on macrophage phagocytic responsegiven that carotenoids function as antioxidants. Carotenoids were isolated,saponified and analysed using HPLC before and after saponification. Murineperitoneal macrophages were cultured in medium containing carotenoidic extract.Carotenoid influence on phagocytosis was assessed by calculating the phagocyticindex (PI % and mean phagocytosis (MP. The positive significant differences(p<0,05 picture carotenoid ability to enhance cell phagocytic activity.

  5. Protective effect of total flavonoids of seabuckthorn (Hippophae rhamnoides) in simulated high-altitude polycythemia in rats.

    Science.gov (United States)

    Zhou, Ji-Yin; Zhou, Shi-Wen; Du, Xiao-Huang; Zeng, Sheng-Ya

    2012-09-28

    Seabuckthorn (Hippophae rhamnoides L.) has been used to treat high altitude diseases. The effects of five-week treatment with total flavonoids of seabuckthorn (35, 70, 140 mg/kg, ig) on cobalt chloride (5.5 mg/kg, ip)- and hypobaric chamber (simulating 5,000 m)-induced high-altitude polycythemia in rats were measured. Total flavonoids decreased red blood cell number, hemoglobin, hematocrit, mean corpuscular hemoglobin levels, span of red blood cell electrophoretic mobility, aggregation index of red blood cell, plasma viscosity, whole blood viscosity, and increased deformation index of red blood cell, erythropoietin level in serum. Total flavonoids increased pH, pO₂, Sp(O₂), pCO₂ levels in arterial blood, and increased Na⁺, HCO₃⁻, Cl⁻, but decreased K⁺ concentrations. Total flavonoids increased mean arterial pressure, left ventricular systolic pressure, end-diastolic pressure, maximal rate of rise and decrease, decreased heart rate and protected right ventricle morphology. Changes in hemodynamic, hematologic parameters, and erythropoietin content suggest that administration of total flavonoids from seabuckthorn may be useful in the prevention of high altitude polycythaemia in rats.

  6. Characterization and identification of ISSR markers associated with oil content in sea buckthorn berries.

    Science.gov (United States)

    Ding, J; Ruan, C J; Guan, Y; Shan, J Y; Li, H; Bao, Y H

    2016-08-19

    Bioactive oils extracted from sea buckthorn (Hippophae rhamnoides) berries contain highly nutritional and medicinal compounds; however, the oil contents of sea buckthorn berries are very low. Thirteen inter-simple sequence repeat (ISSR) primers were used to identify markers associated with oil content of dry pulp in 51 cultivars and lines, which clustered into three major groups based on 137 polymorphic markers. Dry pulp oil contents in 45 cultivars and lines in Group I ranged from 6.6 to 33.1%; these accessions belonged to H. rhamnoides ssp mongolica and its hybrids with H. rhamnoides ssp sinensis. Three lines (H. rhamnoides ssp mongolica) in Group II had high dry pulp oil contents (33.7 to 37.5%), whereas three lines of hybrids in Group III had low dry pulp oil contents (10.9 to 17.5%). The dry pulp oil content of H. rhamnoides ssp mongolica (27.2 ± 0.9%) was higher than that of hybrids (12.0 ± 1.2%) (P sea buckthorn berries.

  7. Effects of aqueous extracts from Panax ginseng and Hippophae rhamnoides on acute alcohol intoxication: An experimental study using mouse model.

    Science.gov (United States)

    Wen, Da-Chao; Hu, Xiao-Yu; Wang, Yan-Yan; Luo, Jian-Xing; Lin, Wu; Jia, Ling-Yan; Gong, Xin-Yue

    2016-11-04

    Acute alcohol intoxication (AAI) is a frequent emergency, but therapeutic drugs with superior efficacy and safety are lacking. Panax ginseng (PG) and Hippophae rhamnoides (HR) respectively has a wide application as a complementary therapeutic agent in China for the treatment of AAI and liver injury induced by alcohol. We investigated the effects of aqueous extracts from PG and HR (AEPH) on AAI mice and identified its underlying mechanisms. Models of AAI were induced by intragastric administration of ethanol (8g/kg). Seventy-two Specific pathogen-free (SPF) male Kunming mice were randomly divided into six groups: normal group, positive control group, AEPH of low dosage (100mg/kg) group, AEPH of medium dose (200mg/kg) group, AEPH of high dosage (400mg/kg) group and model group. The mice were treated with metadoxine (MTD, 500mg/kg) and AEPH. Thirty minutes later, the normal group was given normal saline, while the other groups were given ethanol (i.g., 8g/kg). The impact of AEPH was observed. In the same way, another seventy-two Kunming mice were randomly divided into six groups equally. The blood ethanol concentration at 0.5, 1, 1.5, 2, 3 and 6h after ethanol intake was determined by way of gas chromatography. The activity of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) and microsomal ethanol oxidase (EO) in liver, and the concentration of β-endorphin (β-EP), leucine-enkephalin (LENK) in the brain were determined by enzyme-linked-immunosorbent serologic assay (ELISA). AEPH markedly prolonged alcohol tolerance time and shortened sober-up time after acute ethanol administration. AEPH decreased blood ethanol levels in six tests after ethanol intake. The 7-day survival rate of AEPH group was obviously superior to model group. AEPH increased the activities of ADH, ALDH, and decreased EO activity in liver. The crucial find was that AEPH markedly decreased β-EP and LENK concentration in the brain. AEPH can markedly increase the levels of ADH, ALDH, decrease

  8. Sea buckthorn as a source of important bioactive compounds in cardiovascular diseases.

    Science.gov (United States)

    Olas, Beata

    2016-11-01

    Hippophae rhamnoides (sea buckthorn) offers many health benefits. It has significant cardioprotective activity and exerts many positive healing effects on the cardiovascular system, including inhibiting blood platelet activation (especially platelet aggregation), lowering cholesterol concentration and blood pressure, and providing antioxidant activity. In addition, sea buckthorn has antibacterial and antiviral properties. The leaves and fruits of the plant, and its oils, are sources of many bioactive substances including vitamins (A, C and E), unsaturated fatty acids, phenolic compounds, especially flavonoids, and phytosterols, which bestow positive effects on the cardiovascular system. This review article summarizes the current knowledge of the biological roles of sea buckthorn in cardiovascular diseases. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Implications of various intensities of radio frequency electromagnetic radiation (462 MHz in the induction of oxidative stress during the germination of Hippophae rhamnoides seeds

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    Calin Lucian Maniu

    2010-02-01

    Full Text Available The data accumulated by now shows that the topic of biological effects of electromagnetic radiation is far from being exhausted. Is undoubtedly that a non-ionizing radiation field maintained on a biological entity has some effects on it. To try shaping issues regarding this, this work aims to study the impact of radiation generated by an emission-reception radio station that emits on 462.6875 MHz frequency. For this purpose, were used Hippophae rhamnoides L. seeds which germinated in the laboratory, under controlled conditions, concentrically arranged around the radiation source, in which case electromagnetic radiation has a different impact. Seed germination lasted 35 days, while the device has continuously worked, and the seeds were constantly irradiated. It was precisely measured the intensity of the magnetic component of the field in all places where the seeds were placed for germination. It was calculated the percentage of germination and it was determined the enzyme activity involved in eliminating the oxidative stress effects. It was found significant variations of the parameters mentioned above in conjunction with the radiation intensity depending on the distance from the source.

  10. Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula

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    Barkat Ali Khan

    2013-12-01

    Full Text Available The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®. The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use.

  11. Augmentation of humoral and cellular immunity in response to Tetanus and Diphtheria toxoids by supercritical carbon dioxide extracts of Hippophae rhamnoides L. leaves.

    Science.gov (United States)

    Jayashankar, Bindhya; Singh, Divya; Tanwar, Himanshi; Mishra, K P; Murthy, Swetha; Chanda, Sudipta; Mishra, Jigni; Tulswani, R; Misra, K; Singh, S B; Ganju, Lilly

    2017-03-01

    Hippophae rhamnoides L. commonly known as Seabuckthorn (SBT), a wild shrub of family Elaegnacea, has extensively used for treating various ailments like skin diseases, jaundice, asthma, lung troubles. SBT leaves have been reported to possess several pharmacological properties including immunomodulatory, antioxidant, anti-inflammatory, antimicrobial and tissue regeneration etc. The present study was undertaken to evaluate the adjuvant property of supercritical carbon dioxide extracts (SCEs 300ET and 350ET) of SBT leaves in balb/c mice immunized with Tetanus and Diphtheria toxoids. The dynamic changes in the immune response were measured in terms of humoral and cell-mediated immune responses. We have seen the effect of SCEs on immunoglobulin subtypes and secondary immune response generation. In addition, the effect of SCEs on antigen specific cellular immunity was evaluated. Our results show that SCEs 300ET and 350ET significantly enhanced antibody titers in response to both TT and DT antigens. The secondary immune response generated was significantly increased in case of TT immunized animals. SCEs also enhanced cytokine levels (IFN-γ, IL-4, TNF-α and IL-1β) and increased lymphoproliferation. Besides, both SCEs did not show any toxic effects. Therefore, the study suggests that SCEs are safe and have potent immunostimulatory activity and hence, seems to be a promising balanced Th1 and Th2 directing immunological adjuvant for various veterinary as well as human vaccines. Copyright © 2017. Published by Elsevier B.V.

  12. Effects of co-fermentation by Saccharomyces cerevisiae and Issatchenkia orientalis on sea buckthorn juice.

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    Negi, Bharti; Dey, Gargi

    2013-06-01

    This work relates to the development of a co-fermented product of sea buckthorn (Hippophae rhamnoides L.) with Saccharomyces cerevisiae and Issatchenkia orientalis. Besides malic acid degradation, the parameters of present production technology were also standardized with emphasis on the retainability of total phenolic content (TPC) of sea buckthorn juice. The effect of co-fermentation on physico-chemical characteristics, organic acids, flavonoids, TPC and antioxidant activities was studied. The high-performance liquid chromatography (HPLC) analysis showed 55% reduction in malic acid content after the co-fermentation of sea buckthorn juice. The TPC of sea buckthorn product was 2.18 g gallic acid equivalent (GAE)/l. The estimated scavenging effect on 2,2-diphenyl-1-picrylhydrazyl free radicals was 2.63 Trolox equivalent (TE) mmol/l. Ferric-reducing antioxidant power and 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulphonic acid) assays also showed that sea buckthorn product was on a par with commercial wines (Cabernet Shiraz and Beaujolais). We conclude that the process of co-fermentation resulted in a significant antioxidant potential of sea buckthorn product.

  13. THE UTILIZATION OF THE SEA BUCKTHORN IN ROMANIA, PAST, PRESENT AND FUTURE

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    Angel PROOROCU

    2013-01-01

    Full Text Available Sea buckthorn is called by the Romanian peasants “berries of the Holy Virgin”. They use from hundreds of years the fruits for health problems. From fruits they are obtained many products in feeding. Romania had great specialists in forestry, horticulture, medicine, bio-chemistry with many studies and aplications of sea buckthorn. In Romania Hippophae rhamnoides L. was the object of many national research programs. Now there are not serious investitures and are not mobilized foreign institutions which may use modern instruments for the obtaining all advantages of this plant. Some institutions, industrial units, particulars continue the tradition with good results, that is not the level which is possible in the actual potential of Romania. It must be a solution of the rehabilitation of thousand of hectares of Romanian denuded grounds.

  14. Flavonoid-enriched extract from Hippophae rhamnoides seed reduces high fat diet induced obesity, hypertriglyceridemia, and hepatic triglyceride accumulation in C57BL/6 mice.

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    Yang, Xin; Wang, Qian; Pang, Zeng-Run; Pan, Meng-Ran; Zhang, Wen

    2017-12-01

    Flavonoid-enriched extract from Hippophae rhamnoides L. (Elaeagnaceae) seed (FSH) has shown beneficial effects in anti-hypertension and lowering cholesterol level. However, evidence for its efficacy in treating obesity is limited. We sought to determine if FSH can reduce body weight and regulate lipid metabolism disorder in high fat diet (HFD)-induced obese mouse model, and to investigate potential molecular targets involved. C57BL/6 mice were fed with HFD for 8 weeks to induce obesity. The modeled mice were divided into four groups and treated with vehicle, rosiglitazone (2 mg/kg), low (100 mg/kg) and high (300 mg/kg) dose of FSH, respectively. Normal control was also used. The treatments were administered orally for 9 weeks. We measured the effect of FSH on regulating body weight, various liver and serum parameters, and molecular targets that are key to lipid metabolism. FSH administration at 100 and 300 mg/kg significantly reduced body weight gain by 33.06 and 43.51%, respectively. Additionally, triglyceride concentration in serum and liver were decreased by 15.67 and 49.56%, individually, after FSH (300 mg/kg) treatment. Upon FSH (100 and 300 mg/kg) treatment, PPARα mRNA expression was upregulated in liver (1.24- and 1.42-fold) and in adipose tissue (1.66- and 1.72-fold). Furthermore, FSH downregulated PPARγ protein level both in liver and adipose tissue. Moreover, FSH inhibited macrophage infiltration into adipose tissues, and downregulated TNFα mRNA expression in adipose tissue (38.01-47.70%). This effect was mediated via regulation of PPARγ and PPARα gene expression, and suppression of adipose tissue inflammation.

  15. Comparison of the contents of various antioxidants of sea buckthorn berries using CE.

    Science.gov (United States)

    Gorbatsova, Jelena; Lõugas, Tiina; Vokk, Raivo; Kaljurand, Mihkel

    2007-11-01

    The increased interest in sea buckthorn (Hippophae rhamnoides L.) made it possible to investigate the antioxidant content in it. To address this issue, the presence of following antioxidant compounds were analyzed: trans-resveratrol, catechin, myricetin, quercetin, p-coumaric acid, caffeic acid, L-ascorbic acid (AA), and gallic acid (linear range of 50-150 micromol/L) in six different varieties of sea buckthorn berries extracts (sea buckthorn varieties: "Trofimovskaja (TR)," "Podarok Sadu (PS)," and "Avgustinka (AV),") received from two local Estonian companies. Trans-Resveratrol, catechin, AA, myricetin, and quercetin were found in extracts of sea buckthorn. Moreover, AA, myricetin, and quercetin contents were quantified. The biggest average AA content was found in TR (740 mg/100 g of dried berries, respectively). Furthermore, the same varieties gave the biggest quercetin content 116 mg/100 g of dried berries, respectively. For analysis, CZE was used and the results were partly validated by HPLC. Statistically no big differences in levels of antioxidants were consistently found in different varieties of sea buckthorn extracts investigated in this work.

  16. Sea Buckthorn Leaves and the Novel Food Evaluation

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    Kauppinen Sanna

    2017-06-01

    Full Text Available Novel food means any food that was not used for human consumption to a significant degree within the European Union before 1997. The novel food regulation (EC 258/97 concerns also foods and food ingredients consisting of or isolated from plants, except the food having a history of safe food use within the European Union before 1997. According to the knowledge thus far, sea buckthorn (Hippophaë rhamnoides L. leaves have not been used to a significant degree as food, food supplement, or spice in European Union before 1997. The new regulation on novel foods (EU No. 2015/2283 (Anonymous, 2015 comes into force in the beginning of 2018. After that also history of safe use in a third country is accepted as information of its traditional use. This means continued use for at least 25 years in the customary diet of a significant number of people. Novel food application has to include the description of the product, production process, characteristics and composition, proposed uses and use levels, anticipated intake, history of its use, absorption, distribution, metabolism, excretion, nutritional and toxicological information and allergenicity. Sea buckthorn leaves have been under active research lately and a lot of information is already available, but safety assessment required for novel food evaluation may still be needed.

  17. Sea Buckthorn Oil—A Valuable Source for Cosmeceuticals

    Directory of Open Access Journals (Sweden)

    Marijana Koskovac

    2017-10-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae. is a thorny shrub that has small, yellow to dark orange, soft, juicy berries. Due to hydrophilic and lipophilic ingredients, berries have been used as food and medicine. Sea buckthorn (SB oil derived from berries is a source of valuable ingredients for cosmeceuticals. The unique combination of SB oil ingredients, in qualitative and quantitative aspects, provides multiple benefits of SB oil for internal and external use. Externally, SB oil can be applied in both healthy and damaged skin (burns or skin damage of different etiology, as it has good wound healing properties. Due to the well-balanced content of fatty acids, carotenoids, and vitamins, SB oil may be incorporated in cosmeceuticals for dry, flaky, burned, irritated, or rapidly ageing skin. There have been more than 100 ingredients identified in SB oil, some of which are rare in the plant kingdom (e.g., the ratio of palmitoleic to γ-linolenic acid. This review discusses facts related to the origin and properties of SB oil that make it suitable for cosmeceutical formulation.

  18. Sensilla on the Antennae and Ovipositor of the Sea Buckthorn Carpenter Moth, Holcocerus hippophaecolus Hua et al (Lepidoptera: Cossidae).

    Science.gov (United States)

    Wang, R; Zhang, L; Xu, L L; Zong, S X; Luo, Y Q

    2015-02-01

    Holcocerus hippophaecolus Hua et al (Lepidoptera: Cossidae) is an important boring pest that damages the sea buckthorn Hippophae rhamnoides. Larvae of H. hippophaecolus cause major losses of this shrub in Northern China, with severe economic and ecological consequences. In this study, we used scanning electron microscopy to investigate the typology, morphology, and distribution of sensilla on the antennae and ovipositor of H. hippophaecolus. In total, seven subtypes of sensilla were found on the antennae, i.e., chaetica, trichodea (two subtypes), basiconica (two subtypes), coeloconica, and Böhm bristles. In addition, three types of sensilla were detected on the ovipositor, i.e., chaetica, trichodea, and basiconica. The identification of these sensilla types could provide morphological evidence to facilitate a better understanding of the host location, mate finding, and oviposition processes of this important species.

  19. Projecting the Range Shifts in Climatically Suitable Habitat for Chinese Sea Buckthorn under Climate Change Scenarios

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    Jinghua Huang

    2017-12-01

    Full Text Available Understanding the impact of climate change on range shifts in climatically suitable habitats of tree species is important for national afforestation planning, which can enhance the adaptation of tree plantation to climate change through movement of tree to follow suitable climatic conditions. Here, we overlap the current and future climate-related ranges of Chinese sea buckthorn (Hippophae rhamnoides subsp. sinensis, an important tree used for afforestation in China, to estimate the range shift in three geographic dimensions (latitude, longitude and elevation between 2000 and 2070, which are projected by the maximum entropy algorithm (MaxEnt under current climate conditions and four climate change scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5. Our results show that the performance of the MaxEnt is highly accurate, with test AUC (area under the receiver operating characteristic curve value of 0.91, Kappa value of 0.83 and predicted accuracy of 92%. About 10.7% area of land in China is climatically suitable for Chinese sea buckthorn plantation. Low representative concentration paths will have more effect on loss of climatic range and less effect on expansion of climatic range for Chinese sea buckthorn, while the impacts of high representative concentration path is the opposite. The centroids of climatic ranges will shift westward or northwestward at the rate of 10.4–22 km per decade, and the centroids of altitude will shift upward at the rate of 43–128 m per decade. The expansion area of climatically suitable habitat, covering 2.6–5.2 × 105 km2, is expected to be mainly located in parts of Qinghai, Ningxia, Gansu, Sichuan, Liaoning, and Jilin provinces; these areas should be monitored for planting of Chinese sea buckthorn in the future.

  20. Evaluation of Limiting Climatic Factors and Simulation of a Climatically Suitable Habitat for Chinese Sea Buckthorn.

    Science.gov (United States)

    Li, Guoqing; Du, Sheng; Guo, Ke

    2015-01-01

    Chinese sea buckthorn (Hippophae rhamnoides subsp. sinensis) has considerable economic potential and plays an important role in reclamation and soil and water conservation. For scientific cultivation of this species across China, we identified the key climatic factors and explored climatically suitable habitat in order to maximize survival of Chinese sea buckthorn using MaxEnt and GIS tools, based on 98 occurrence records from herbarium and publications and 13 climatic factors from Bioclim, Holdridge life zone and Kria' index variables. Our simulation showed that the MaxEnt model performance was significantly better than random, with an average test AUC value of 0.93 with 10-fold cross validation. A jackknife test and the regularized gain change, which were applied to the training algorithm, showed that precipitation of the driest month (PDM), annual precipitation (AP), coldness index (CI) and annual range of temperature (ART) were the most influential climatic factors in limiting the distribution of Chinese sea buckthorn, which explained 70.1% of the variation. The predicted map showed that the core of climatically suitable habitat was distributed from the southwest to northwest of Gansu, Ningxia, Shaanxi and Shanxi provinces, where the most influential climate variables were PDM of 1.0-7.0 mm, AP of 344.0-1089.0 mm, CI of -47.7-0.0°C, and ART of 26.1-45.0°C. We conclude that the distribution patterns of Chinese sea buckthorn are related to the northwest winter monsoon, the southwest summer monsoon and the southeast summer monsoon systems in China.

  1. Evaluation of Limiting Climatic Factors and Simulation of a Climatically Suitable Habitat for Chinese Sea Buckthorn.

    Directory of Open Access Journals (Sweden)

    Guoqing Li

    Full Text Available Chinese sea buckthorn (Hippophae rhamnoides subsp. sinensis has considerable economic potential and plays an important role in reclamation and soil and water conservation. For scientific cultivation of this species across China, we identified the key climatic factors and explored climatically suitable habitat in order to maximize survival of Chinese sea buckthorn using MaxEnt and GIS tools, based on 98 occurrence records from herbarium and publications and 13 climatic factors from Bioclim, Holdridge life zone and Kria' index variables. Our simulation showed that the MaxEnt model performance was significantly better than random, with an average test AUC value of 0.93 with 10-fold cross validation. A jackknife test and the regularized gain change, which were applied to the training algorithm, showed that precipitation of the driest month (PDM, annual precipitation (AP, coldness index (CI and annual range of temperature (ART were the most influential climatic factors in limiting the distribution of Chinese sea buckthorn, which explained 70.1% of the variation. The predicted map showed that the core of climatically suitable habitat was distributed from the southwest to northwest of Gansu, Ningxia, Shaanxi and Shanxi provinces, where the most influential climate variables were PDM of 1.0-7.0 mm, AP of 344.0-1089.0 mm, CI of -47.7-0.0°C, and ART of 26.1-45.0°C. We conclude that the distribution patterns of Chinese sea buckthorn are related to the northwest winter monsoon, the southwest summer monsoon and the southeast summer monsoon systems in China.

  2. Assessment of genetic diversity using RAPD analysis in a germplasm collection of sea buckthorn

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    G.I. BARTISH

    2008-12-01

    Full Text Available Random amplified polymorphic DNA (RAPD markers were used to characterize a part of a sea buckthorn gene bank collected for plant breeding purposes. Molecular markers were generated in 55 cultivars and accessions, representing five subspecies of Hippophae rhamnoides L. and intraspecific hybrids between different subspecies. Sixty-three markers were used to generate a Dice's similarity coefficient matrix of pairwise comparisons between individual RAPD profiles. Cluster (UPGMA and principal co-ordinate analyses, based on this matrix, revealed clustering of plants into groups which generally correspond to their taxonomic classification or geographic origin. The analysis of molecular variance (AMOVA was found useful for estimating components of genetic variation between and within taxonomic and geographic groups of accessions and cultivars. Whereas both alternatives for grouping the material (taxonomic or geographic origin resulted in significant between-group variation, the major part of molecular variance (approximately 75% was still attributed to variation within groups. We conclude that the RAPD analysis is useful for clarification of taxonomic and geographic origin of accessions and cultivars of sea buckthorn.

  3. Využití některých méňě pěstovaných ovocných druhů pro zpracování

    OpenAIRE

    Kotrašová, Petra

    2014-01-01

    The topic of the thesis deals with the use of less cultivated fruit species for processing. There were three kinds of fruit processing jujube (Ziziphus jujuba), elderberry (Sambucus nigra) and sea buckthorn (Hippophae rhamnoides). Ziziphus jujube fruit species was processed into jujube jam, compote of dried jujube and jujube . The sea buckthorn (Hippophae rhamnoides) was served in prodicts of sea buckthorn juice, syrup of buckthorn, sea buckthorn jam and cakes from sea buckthorn. Elderberry p...

  4. CYTOGENETIC STUDIES ON SEVERAL HIPPOPHAƠ RHAMNOIDES L. GENOTYPES

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    Diana-Elena Maftei

    2012-06-01

    Full Text Available Sea-buckthorn (Hippophaë rhamnoides (2n=24 is a dioecious plant with a very obvious morpho-physiological polymorphism. This species is one of the most valuable fruit-bearing shrubs of the spontaneous and also of the cultivated flora, due to its content of biologically active substances of its leaves, fruit and shoots. The research has been accomplished on root apical meristems from germinated seeds that belong to four genotypes characteristic for Bacău county - Dospineúti, ùerpeni 11, ùerbăneúti 4, Sfiútofca 18, and to one genotype of the Danube Delta - Sfântul Gheorghe 5. Cytogenetical studies evinced that the mitotic index (MI was high, and it varied with the genotype. The highest MI was evinced in the ùerbăneúti 4 genotype (53.13, and the lowest – in ùerpeni 11 (40.08. Cell distribution per mitotic phases is approximately the same, the highest percentage was represented by cells in prophase, followed by metaphases, telophases and anaphases. There is a rather high frequency and a quite large spectrum of  chromosomal aberrations identified in this species (with variations due to the 5 different genotypes. Of all the chromosomal aberrations evinced during the ana-telophases of mitotic root apical meristems (bridges, delayed chromosomes, expelled chromosomes, fragments, micronuclei, the highest frequency was represented by ana-telophases with bridges. There has been noticed the presence of chromosomal abnormalities in metaphasic and prophasic cells.

  5. High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn.

    Science.gov (United States)

    Zhao, Lu; Wen, E; Upur, Halmuart; Tian, Shuge

    2017-01-01

    Sea buckthorn ( Hippophae rhamnoides L.) as a traditional Chinese medicinal plant has various uses in Xinjiang. A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes ( R 2 > 0.9997). The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang. The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn. Quantitative analysis method of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the extract of sea buckthorn pulp and seed is developed by high-performance liquid chromatography (HPLC) diode array detection.This method is simple and accurate; has strong specificity, good precision, and high recovery rate; and provides a reliable basis for further development of the substances in the pulp and seed of sea buckthorn.The method is widely used for content determination of active ingredients or physiologically active components in traditional Chinese medicine and its preparation Abbreviation used: PR: protocatechuic acid, RU

  6. Sea Buckthorn Leaf Extract Inhibits Glioma Cell Growth by Reducing Reactive Oxygen Species and Promoting Apoptosis.

    Science.gov (United States)

    Kim, Sung-Jo; Hwang, Eunmi; Yi, Sun Shin; Song, Ki Duk; Lee, Hak-Kyo; Heo, Tae-Hwe; Park, Sang-Kyu; Jung, Yun Joo; Jun, Hyun Sik

    2017-08-01

    Hippophae rhamnoides L., also known as sea buckthorn (SBT), possesses a wide range of biological and pharmacological activities. However, the underlying mechanism is largely unknown. The present study examined whether SBT leaf extract could inhibit proliferation and promote apoptosis of rat glioma C6 cells. The results revealed that the treatment with SBT leaf extract inhibited proliferation of rat C6 glioma cells in a dose-dependent manner. SBT-induced reduction of C6 glioma cell proliferation and viability was accompanied by a decrease in production of reactive oxygen species (ROS), which are critical for the proliferation of tumor cells. SBT treatment not only significantly upregulated the expression of the pro-apoptotic protein Bcl-2-associated X (Bax) but also promoted its localization in the nucleus. Although increased expression and nuclear translocation of Bax were observed in SBT-treated C6 glioma cells, the induced nuclear morphological change was distinct from that of typical apoptotic cells in that most of SBT-treated cells were characterized by convoluted nuclei with cavitations and clumps of chromatin. All of these results suggest that SBT leaf extract could inhibit the rapid proliferation of rat C6 glioma cells, possibly by inducing the early events of apoptosis. Thus, SBT may serve as a potential therapeutic candidate for the treatment of glioma.

  7. Proteome profiling reveals insights into cold-tolerant growth in sea buckthorn.

    Science.gov (United States)

    He, Caiyun; Gao, Guori; Zhang, Jianguo; Duan, Aiguo; Luo, Hongmei

    2016-01-01

    Low temperature is one of the crucial environmental factors limiting the productivity and distribution of plants. Sea buckthorn ( Hippophae rhamnoides L.), a well recognized multipurpose plant species, live successfully in in cold desert regions. But their molecular mechanisms underlying cold tolerance are not well understood. Physiological and biochemical responses to low-temperature stress were studied in seedlings of sea buckthorn. Differentially expressed protein spots were analyzed using multiplexing fluorescent two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry (MS), the concentration of phytohormone was measured using enzyme-linked immunosorbent assay, and a spectrophotometric assay was used to measure enzymatic reactions. With the increase of cold stress intensity, the photosynthesis rate, transpiration rate, stomatal conductance in leaves and contents of abscisic acid (ABA) and indole acetic acid (IAA) in roots decreased significantly; however, water-use efficiency, ABA and zeatin riboside in leaves increased significantly, while cell membrane permeability, malondialdehyde and IAA in leaves increased at 7 d and then decreased at 14 d. DIGE and MS/MS analysis identified 32 of 39 differentially expressed protein spots under low-temperature stress, and their functions were mainly involved in metabolism, photosynthesis, signal transduction, antioxidative systems and post-translational modification. The changed protein abundance and corresponding physiological-biochemical response shed light on the molecular mechanisms related to cold tolerance in cold-tolerant plants and provide key candidate proteins for genetic improvement of plants.

  8. Cutaneous Effects of Sea Buckthorn Oil Emulsion

    Directory of Open Access Journals (Sweden)

    Kipic Mihailo

    2014-10-01

    Full Text Available Ulje pasjeg trna (Hippophae rhamnoides L. se u medicinske svrhe upotrebljava kako za eksternu tako i za internu primenu, pri čemu je eksterna primena nepogodna usled njegove tečne konzistencije, lipofi lne prirode i intenzivne obojenosti. Navedeni nedostaci bi se mogli prevazići formulacijom polučvrstih emulzija sa uljem pasjeg trna. Prethodna ispitivanja ove formulacije su pokazala da poseduje znatno veći potencijal za zarastanje rana u odnosu na ulje pasjeg trna, unapređenu strukturu tečnih kristala, stabilnost i pogodnost za lokalnu primenu.

  9. Effects of oral sea buckthorn oil on tear film Fatty acids in individuals with dry eye.

    Science.gov (United States)

    Järvinen, Riikka L; Larmo, Petra S; Setälä, Niko L; Yang, Baoru; Engblom, Janne Rk; Viitanen, Matti H; Kallio, Heikki P

    2011-09-01

    Evaporative dry eye is associated with meibomian gland dysfunction and abnormalities of the tear film lipids. Dry eye is known to be affected positively by intake of linoleic and γ-linolenic acids and n-3 fatty acids. Oral sea buckthorn (Hippophaë rhamnoides) (SB) oil, which contains linoleic and α-linolenic acids and antioxidants, has shown beneficial effects on dry eye. The objective was to investigate whether supplementation with SB oil affects the composition of the tear film fatty acids in individuals reporting dry eye. One hundred participants were randomized to this parallel, double-blind, placebo-controlled study, which 86 of them completed. The participants daily consumed 2 g of SB or placebo oil for 3 months. Tear film samples were collected at the beginning, during, and at the end of the intervention and 1 to 2 months later. Tear film fatty acids were analyzed as methyl esters by gas chromatography. There were no group differences in the changes in fatty acid proportions during the intervention (branched-chain fatty acids: P = 0.49, saturated fatty acids: P = 0.59, monounsaturated fatty acids: P = 0.53, and polyunsaturated fatty acids: P = 0.16). The results indicate that the positive effects of SB oil on dry eye are not mediated through direct effects on the tear film fatty acids. Carotenoids and tocopherols in the oil or eicosanoids produced from the fatty acids of the oil may have a positive effect on inflammation and differentiation of the meibomian gland cells.

  10. Protective effects of sea buckthorn polysaccharide extracts against LPS/d-GalN-induced acute liver failure in mice via suppressing TLR4-NF-κB signaling.

    Science.gov (United States)

    Liu, Huan; Zhang, Wei; Dong, Shichao; Song, Liang; Zhao, Shimin; Wu, Chunyan; Wang, Xue; Liu, Fang; Xie, Jiming; Wang, Jinling; Wang, Yuzhen

    2015-12-24

    Sea buckthorn (Hippophae rhamnoides L.) berries have been traditionally used to treat gastric disorders, cardiovascular problems, and liver injuries in oriental medicinal system. This study aimed to explore the protective effects and mechanisms of the polysaccharide extracts of Sea buckthorn (HRP) berries against lipopolysaccharide (LPS) and d-galactosamine hydrochloride (d-GalN)-induced acute liver failure in mice. HRP was isolated by hot-water extraction and characterized by HPLC and infrared spectrum analysis. The total carbohydrate, uronic acid and protein contents of HRP were measured by a spectrophotometric method. Mice were orally administrated with HRP (50, 100, 200mg/kg) once daily for 14 consecutive days prior to the challenge with LPS (50 μg/kg) and d-GalN (300 mg/kg). Animals of positive control group were intraperitoneally injected with dexamethasone (10mg/kg). Mice were sacrificed at 8h after LPS/d-GalN injection. Pretreatment with HRP significantly inhibited LPS/d-GalN-induced increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, which were accompanied by alleviated liver injuries and reduced production of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). HRP was also found to reduce malondialdehyde (MDA) content and to restore superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities. Furthermore, HRP supplementation dose-dependently inhibited the expression of Toll-like receptor 4 (TLR4), phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated c-Jun N-terminal kinase (p-JNK), and phosphorylated mitogen activated protein kinase 38 (p-p38 MAPK) in the liver of LPS/d-GalN challenged mice. Pretreatment with HRP also inhibited LPS/d-GalN-induced activation and translocation of nuclear factor-κB (NF-κB). This study indicates that pretreatment with HRP protects against LPS/d-GalN-induced liver injury in mice via suppressing the TLR4-NF-κB signaling pathway. Sea

  11. Effect of sea buckthorn berries and pulp in a liquid emulsion on gastric ulcer scores and gastric juice pH in horses.

    Science.gov (United States)

    Huff, N K; Auer, A D; Garza, F; Keowen, M L; Kearney, M T; McMullin, R B; Andrews, F M

    2012-01-01

    Sea buckthorn berries (Hippophae rhamnoides) are rich in vitamin C and E, carotenoids, flavonoids, fatty acids, plant sterols, lignans, and minerals. A feed supplement containing sea buckthorn berries might have efficacy in treatment and prevention of gastric ulcers in horses. To test the efficacy of a commercially available formulation of sea buckthorn berries and pulp (SeaBuck SBT Gastro-Plus) for treatment and prevention of gastric ulcers in stall-confined horses. Eight Thoroughbred and Thoroughbred-cross horses (3-10 years of age, 5 geldings and 3 mares, 380-600 kg body weight). This study was a 2-period crossover in which all horses received no treatment (untreated controls; n = 8) and treatment (SeaBuckSBT Gastro-Plus, 4 ounces [35.6 g berries and pulp], twice daily; n = 8) mixed with a pelleted complete feed (18% crude fiber; 9% starch; 14% crude protein). Horses were treated for 4 weeks followed by a 1-week (d28-d35) alternating feed-deprivation period to induce or worsen existing ulcers. Gastroscopic examinations were performed on days 0, 28, and 35. Gastric juice pH was measured and gastric ulcer number and severity scores were assigned by a masked investigator. Mean nonglandular gastric ulcer scores significantly (P < .05) increased in all horses after day 28, as a result of intermittent feed deprivation. Mean nonglandular gastric ulcer number (P = .84) and severity (P = .51) were not significantly different between SBT-treated and untreated control horses. However, mean glandular ulcer number (P = .02) and glandular ulcer severity (P = .02) were significantly lower in the SBT-treated horses compared with the untreated control at week 5. SeaBuck SBT Gastro-Plus liquid fed to horses did not show efficacy in treatment or prevention of naturally occurring nonglandular ulcers in horses; however, glandular ulcer scores were significantly lower in SBT-treated horses after feed deprivation. Thus, SBT might have efficacy in prevention of glandular ulcers in

  12. Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves.

    Science.gov (United States)

    Ganju, Lilly; Padwad, Yogendra; Singh, Richa; Karan, Dev; Chanda, Sudipta; Chopra, Mohinder Kumar; Bhatnagar, Parul; Kashyap, Ravi; Sawhney, Ramesh Chandra

    2005-11-01

    Immunomodulatory activity of Seabuckthorn (SBT) leaf extract was evaluated in adjuvant induced arthritis (AIA) rat model. Inflammation was induced by injecting Complete Freund's Adjuvant (CFA) in the right hind paw of rats. SBT extract was administered intraperitoneally to treat the inflammation. The extent of inflammation and treatment response was evaluated by clinical analysis, scintigraphic visualization using technitium-99m-glutathione (Tc99m-GSH) and lymphocyte proliferation. Serial evaluation was carried out on days 1, 7, 14, 21 and 28 after creation of inflammation. The Tc99m-GSH uptake in the inflamed leg was compared with the normal contralateral leg of the same animal. The measurements were done by obtaining scintigraphic images using gamma camera and an online computer. Both qualitative and quantitative evaluation of radiotracer accumulation was considered to evaluate the anti-inflammatory response. The lymphocyte proliferation study revealed cellular immunosuppression during the early phase of the disease. Administration of SBT extract on the same day or 5 days prior to inflammatory insult into the joint, significantly reduced the inflammation as compared to the untreated animals in a dose dependent manner. These observations suggest that the SBT leaf extract has a significant anti-inflammatory activity and has the potential for the treatment of arthritis.

  13. Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes.

    Science.gov (United States)

    Podder, Biswajit; Kim, Yong-Sik; Song, Ho-Yeon

    2013-12-01

    The extract of sea buckthorn (SBT) [Hippophae rhamnoides L. (Elaeagnaceae)], is used as a food supplement and traditional medicine in numerous countries. This study investigated the protective effects of the functional extract of SBT against paraquat (PQ)-induced toxicity via antioxidant mechanisms in A549 cells. The methanol extract of SBT (25-200 µg/ml) was used to protect cells against PQ (200 µM)-induced cell death. A viability assay was conducted using 3-(4,5-dimethylthioazol-2-ly)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase (LDH). Total intracellular reactive oxygen species (ROS) were measured and plotted. For validation of the SBT-induced expression of nuclear factor-E2-related factor 2 (Nrf2) and its target genes, western blot analysis and qPCR were performed. The present study showed that pretreatment of A549 cells with SBT extract significantly attenuated PQ (200 µM)-induced cellular toxicity. The maximum cytoprotective effect was identified using 200 µg/ml SBT extract; it began 24 h following exposure and was sustained up to 120 h (P<0.05). SBT extract significantly reduced LDH activity by 35.63% and ROS levels by 30.90% (P<0.05). Pretreatment with SBT extract activated Nrf2 mRNA and protein expression and its nuclear translocation. The SBT extract effectively induced Nrf2 target genes, such as NAD(P)H dehydrogenase quinone 1, glutathione peroxidase 1, glutathione reductase and catalase following treatment with PQ. Based on these results, it was hypothesized that SBT extract may be used as a potential therapeutic agent for the treatment of various oxidative stress-related diseases.

  14. Formulation and evaluation of antisebum secretion effects of sea buckthorn w/o emulsion.

    Science.gov (United States)

    Akhtar, Naveed; Khan, Barkat A; Mahmood, Tariq; Parveen, Rashida; Qayum, Mughal; Anwar, Masood; Shahiq-Uz-Zaman; Farooq, Muhammad

    2010-01-01

    This study was designed to formulate and evaluate the anti-sebum secretion effects of a topical skin-care cream (w/o emulsion) of sea buckthorn versus its vehicle (Base) as control. Concentrated sea buckthorn (H.rhamnoides) fruit extract was entrapped in the inner aqueous phase of w/o emulsion. Base containing no extract and a Formulation containing 1% concentrated extract of H.rhamnoides was formulated. Lemon oil was incorporated to the odor. Both the Base and the Formulation were stored at different storage conditions for a period of 4 weeks to predict their stability. Different stability parameters i.e.; physical stability, centrifugation, and pH were monitored at different time intervals. Both the Base and the Formulation were applied to the cheeks of 10 healthy human volunteers (n=10) for a period of 8 weeks. The expected organoleptic stability of creams was achieved from 4 weeks in-vitro study period. Odor disappeared with the passage of time due to volatilization of lemon oil. The pH of the Formulation showed significant (P = 0.0002) decline due to high concentration of organic acids present in sea buckthorn. Similarly the Formulation showed statistically significant (P < 0.05) effects on skin sebum secretion. The in vitro results showed a good stability over 4 weeks of observation period of both the Base and Formulation and the Formulation has anti sebum secretion effects over 8 weeks of observation period.

  15. Formulation and evaluation of antisebum secretion effects of sea buckthorn w/o emulsion

    Directory of Open Access Journals (Sweden)

    Naveed Akhtar

    2010-01-01

    Full Text Available Purpose : This study was designed to formulate and evaluate the anti-sebum secretion effects of a topical skin-care cream (w/o emulsion of sea buckthorn versus its vehicle (Base as control. Materials and Methods : Concentrated sea buckthorn (H.rhamnoides fruit extract was entrapped in the inner aqueous phase of w/o emulsion. Base containing no extract and a Formulation containing 1% concentrated extract of H.rhamnoides was formulated. Lemon oil was incorporated to the odor. Both the Base and the Formulation were stored at different storage conditions for a period of 4 weeks to predict their stability. Different stability parameters i.e.; physical stability, centrifugation, and pH were monitored at different time intervals. Both the Base and the Formulation were applied to the cheeks of 10 healthy human volunteers (n=10 for a period of 8 weeks. Result : The expected organoleptic stability of creams was achieved from 4 weeks in-vitro study period. Odor disappeared with the passage of time due to volatilization of lemon oil. The pH of the Formulation showed significant ( P = 0.0002 decline due to high concentration of organic acids present in sea buckthorn. Similarly the Formulation showed statistically significant ( P < 0.05 effects on skin sebum secretion. Conclusion : The in vitro results showed a good stability over 4 weeks of observation period of both the Base and Formulation and the Formulation has anti sebum secretion effects over 8 weeks of observation period.

  16. Investigating the antioxidant properties and rutin content of Sea ...

    African Journals Online (AJOL)

    The present study focused on the antioxidant properties and rutin content of leaves and branches of Hippophae rhamnoides L. (Sea buckthorn) in Turkey. Dried leaves (leaf tea), processed (PB) and unprocessed branches (UB) of Sea buckthorn (SBT) were extracted with ethanol and prepared in forms of aqueous extract ...

  17. A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model.

    Science.gov (United States)

    İlhan, Mert; Süntar, İpek; Demirel, Mürşide Ayşe; Yeşilada, Erdem; Keleş, Hikmet; Küpeli Akkol, Esra

    2016-12-01

    Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mixture of sea buckthorn and St. John's wort oils (HrHp oil) in the treatment of endometriosis. The activity was assessed in surgically induced endometriosis in rats. A 15-mm piece of endometrium was sutured into the abdominal wall. Twenty-eight days later, a second laparotomy was performed to calculate the endometrial foci areas and to score intra-abdominal adhesions. The rats were treated with either vehicle, HrHp oil formulation, or the reference (buserelin acetate). At the end of the experiment all rats were sacrificed and endometriotic foci areas and intra-abdominal adhesions were re-evaluated. The tissue sections were analyzed histopathologically. Peritoneal fluids of the experimental animals were collected in order to detect the levels of tumor necrosis factor-α, vascular endothelial growth factor, and interleukin-6, which might be involved in the etiology of endometriosis. In the HrHp oil-treated group, the volumes of endometriotic implants were found to be significantly decreased (from 50.8 mm 3 to 18.6 mm 3 , p<0.001) without any adhesion (0.0±0.0, p<0.001) when compared to the control group (3.1±0.9). The levels of tumor necrosis factor-α decreased from 7.02±1.33 pg/mL to 4.78±1.02 pg/mL (p<0.01); vascular endothelial growth factor from 17.39±8.52 pg/mL to 9.67±5.04 pg/mL (p<0.01); and interleukin-6 from 50.95±22.84 pg/mL to 29.11±7.45 pg/mL (p<0.01), respectively, after HrHp oil treatment. HrHp oil may be a promising alternative for the treatment of endometriosis. Copyright © 2016. Published by Elsevier B.V.

  18. Flavonoidy a další biologicky aktivní látky v rakytníku

    OpenAIRE

    Michalíková, Adéla

    2014-01-01

    This bachelor thesis summarizes information about sea buckthorn (Hippophae rhamnoides), about chemical composition of different parts and products based on this species, and about their biologically active contents. A special emphasis is given to the oil prepared from sea buckthorn fruits. Sea buckthorn is a rich source of antioxidants such as vitamin C, vitamin E, flavonoids and carotenoids. The oil contains high levels of unsaturated fatty acids, in particular of palmitooleic acid. The bene...

  19. Wound healing potential of a polyvinyl alcohol-blended pectin hydrogel containing Hippophae rahmnoides L. extract in a rat model.

    Science.gov (United States)

    Kim, Jin; Lee, Chang-Moon

    2017-06-01

    In this study, we investigated the effect of a polyvinyl alcohol-blended pectin hydrogel (PVA-PT HG) containing the extracts of Hippophae rhamnoides L. (H. rhamnoides L.) leaves on wound healing in a rat model. The total phenolic content in the extract solution was 40.64±2.7 GAE mg/g and that of flavonoids was 13.15±1.8 QE mg/g. Of the total flavonoids in HGs, 61.6 and 50.0% were released at pH 5.5 and 7.4 after 60min. In rat acute wound models, the wound size was reduced significantly and the recovery rate was significantly higher after treatment with HG containing the extracts, compared with treatment with the control and HG only. The wound healing effects of the HG containing the extracts were confirmed by histological evaluation of the wound tissue. Therefore, HG containing extracts from H. rhamnoides L. leaves enhanced wound healing effectively, and so may be developed as a cover to promote wound healing. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Comparative chemical composition, antioxidant and anticoagulant properties of phenolic fraction (a rich in non-acylated and acylated flavonoids and non-polar compounds) and non-polar fraction from Elaeagnus rhamnoides (L.) A. Nelson fruits.

    Science.gov (United States)

    Olas, Beata; Żuchowski, Jerzy; Lis, Bernadetta; Skalski, Bartosz; Kontek, Bogdan; Grabarczyk, Łukasz; Stochmal, Anna

    2018-05-01

    This study focuses on two fractions from sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) fruits: the phenolic fraction (rich in non-acylated and acylated flavonoids and non-polar compounds) and the non-polar fraction. The objective was to investigate both the chemical composition of these fractions, as well as their biological activities in vitro. The tested fractions of sea buckthorn inhibited lipid peroxidation induced by H2O2, however, the non-polar fraction reduced more powerfully the process induced by H2O2/Fe as compared to the phenolic fraction. The tested fractions of sea buckthorn fruits also inhibited carbonylation stimulated by H2O2/Fe. Moreover, the action of the phenolic fraction and non-polar fraction on hemostatic parameters of plasma was also compared to activities of other phenolic fraction, in which flavonoids were the dominant compounds. Our results indicate that sea buckthorn fruits are a rich source of different secondary metabolites, i.e. triterpenes and their derivates, which possess not only antioxidant properties, but may also display anticoagulant attributes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Microbial Safety Improvement of Sea Buckthorn by Electron Beam Irradiation

    Science.gov (United States)

    Nemţanu, Monica R.; Minea, R.; Mazilu, Elena; Rǎdulescu, Nora

    2007-04-01

    The commercialization of medicinal plants and/or their products is highly increased in Romania lately. One of the most used herbs is sea buckthorn being well known for its quality with a large potential for curing some diseases. Sea buckthorn can be contaminated with undesirable microorganisms which may affect negatively its quality. The paper presents the results regarding the action of a non-conventional technology meaning electron beam technique on sea buckthorn in order to improve its microbiological quality. Our study revealed that the sea buckthorn microbial load has been improved after 3 kGy irradiation keeping its active principles.

  2. Antioxidant activity and free radicals of roasted herbal materials

    Directory of Open Access Journals (Sweden)

    Wojtowicz Elżbieta

    2017-06-01

    Full Text Available Introduction: Novel raw materials are being constantly searched for chicory coffee, which thanks to their specific composition can influence human health, thus promoting properties and of course the attractive aroma. Prior to their addition herbs – sea buckthorn (Hippophaë rhamnoides L., rowanberry (Sorbus aucuparia L., lovage roots (Levisticum officinale Koch and dandelion (Taraxacum officinale coll. – are roasted, which may change their antioxidant properties and generate free radicals with pro-oxidative properties.

  3. Možnosti využití plodů netradičních ovocných druhů

    OpenAIRE

    Gőttingerová, Martina

    2016-01-01

    The topic of this bachelor thesis is to study the possibilies of the use of minor fruits and its products. Three species of fruits were proccesed: elderberry (Sambucus nigra L.), cornelian cherry (Cornus mas L.) and sea buckthorn Hippophae rhamnoides L.). The fruit of the elderberry (Sambucus nigra L.) were processed to makeelderberry syrup, compote and jelly. To compare the flavors a elderberry syrup was purchased. The fruit of the cornelian cherry (Cornus mas L.) was processed to make corn...

  4. SPECTROSCOPIC STUDY OF SEA BUCKTHORN EXTRACTS

    Directory of Open Access Journals (Sweden)

    Carmen Mihaela Topală

    2014-11-01

    Full Text Available The application of sea buckthorn oil is to incorporate the oil into foodstuffs such as milk, yoghurt, cheese, butter, juice and snacks which represents new opportunities for food manufacturers, food supplements and nutraceuticals providing nutritional supports. The FTIR spectroscopy is a powerful technique for assessing food production and studied materials provides fundamental information on the behavior of the spectral metabolites and bio product. The extracts were studied from two varieties of sea buckthorn oil Pitesti I and II. Oil obtained from peel and seeds by the Soxhlet extraction with hexane solvent and CO2 supercriticalwas analyzed by FTIR spectroscopy. The concentration of fatty acids in oil extracted from seeds and peels was similar in both extraction techniques.

  5. Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

    Czech Academy of Sciences Publication Activity Database

    Puterová, J.; Razumova, O.; Martínek, T.; Alexandrov, O.; Divashuk, M.; Kubát, Z.; Hobza, Roman; Karlov, G.; Kejnovský, E.

    2017-01-01

    Roč. 9, č. 1 (2017), s. 197-212 ISSN 1759-6653 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:61389030 Keywords : sex-chromosomes * repetitive sequences * silene-latifolia * molecular cytogenetics * arabidopsis-thaliana * genome size * evolution * organization * alignment * database * sex chromosomes * genome composition * chromosomal localization * repetitive DNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.979, year: 2016

  6. Assessment of antimycotic activity of seabuckthorn (Hippophae rhamnoides leaf exacts against common fungi associated with skin dermatitis

    Directory of Open Access Journals (Sweden)

    Harshit Verma

    2013-08-01

    Full Text Available Aim: To study the antimycotic activity of seabuckthorn leaf extracts against common fungi associated with skin infection. Materials and Methods: A total of 31 isolates were collected from the skin cases of animals and the leaves of seabuckthorn collected from the Lahaul and Spiti valley of the Himachal Pradesh. The extracts from the leaves were extracted according to standard procedures. Antimycotic activity was examined by employing agar disc diffusion method on Roswell Park Memorial Institute medium (RPMI. Different concentrations of leaf extracts i.e. 0.50%, 2%, 3%, 4%, 5%, respectively were used. Results: The study revealed that the inhibitory effect of SBT leaf extract at 5% concentration was observed to be almost 80% in 12 hrs of reading as compared to the standard drugs used as positive control against fungal isolates. Conclusion: Plants are important source of potentially useful structures for the development of new chemotherapeutic agents. The first step towards this goal is the in vitro antimycotic activity assay. In the present study, a wide range of pathogenic microorganisms were recovered from the clinical samples, including bacteria and fungus. The seabuckthorn methanolic leaf extracts (hot and cold were studied for their antimycotic activity against common fungal pathogens. [Vet World 2013; 6(4.000: 205-208

  7. Refolding of β-stranded class I chitinases of Hippophae rhamnoides enhances the antifreeze activity during cold acclimation.

    Directory of Open Access Journals (Sweden)

    Ravi Gupta

    Full Text Available Class I chitinases hydrolyse the β-1,4-linkage of chitin and also acquire antifreeze activity in some of the overwintering plants during cold stress. Two chitinases, HrCHT1a of 31 kDa and HrCHT1b of 34 kDa, were purified from cold acclimated and non-acclimated seabuckthorn seedlings using chitin affinity chromatography. 2-D gels of HrCHT1a and HrCHT1b showed single spots with pIs 7.0 and 4.6 respectively. N-terminal sequence of HrCHT1b matched with the class I chitinase of rice and antifreeze proteins while HrCHT1a could not be sequenced as it was N-terminally blocked. Unlike previous reports, where antifreeze activity of chitinase was cold inducible, our results showed that antifreeze activity is constitutive property of class I chitinase as both HrCHT1a and HrCHT1b isolated even from non-acclimated seedlings, exhibited antifreeze activity. Interestingly, HrCHT1a and HrCHT1b purified from cold acclimated seedlings, exhibited 4 and 2 times higher antifreeze activities than those purified from non-acclimated seedlings, suggesting that antifreeze activity increased during cold acclimation. HrCHT1b exhibited 23-33% higher hydrolytic activity and 2-4 times lower antifreeze activity than HrCHT1a did. HrCHT1b was found to be a glycoprotein; however, its antifreeze activity was independent of glycosylation as even deglycosylated HrCHT1b exhibited antifreeze activity. Circular dichroism (CD analysis showed that both these chitinases were rich in unusual β-stranded conformation (36-43% and the content of β-strand increased (∼11% during cold acclimation. Surprisingly, calcium decreased both the activities of HrCHT1b while in case of HrCHT1a, a decrease in the hydrolytic activity and enhancement in its antifreeze activity was observed. CD results showed that addition of calcium also increased the β-stranded conformation of HrCHT1a and HrCHT1b. This is the first report, which shows that antifreeze activity is constitutive property of class I chitinase and cold acclimation and calcium regulate these activities of chitinases by changing the secondary structure.

  8. Refolding of β-stranded class I chitinases of Hippophae rhamnoides enhances the antifreeze activity during cold acclimation.

    Science.gov (United States)

    Gupta, Ravi; Deswal, Renu

    2014-01-01

    Class I chitinases hydrolyse the β-1,4-linkage of chitin and also acquire antifreeze activity in some of the overwintering plants during cold stress. Two chitinases, HrCHT1a of 31 kDa and HrCHT1b of 34 kDa, were purified from cold acclimated and non-acclimated seabuckthorn seedlings using chitin affinity chromatography. 2-D gels of HrCHT1a and HrCHT1b showed single spots with pIs 7.0 and 4.6 respectively. N-terminal sequence of HrCHT1b matched with the class I chitinase of rice and antifreeze proteins while HrCHT1a could not be sequenced as it was N-terminally blocked. Unlike previous reports, where antifreeze activity of chitinase was cold inducible, our results showed that antifreeze activity is constitutive property of class I chitinase as both HrCHT1a and HrCHT1b isolated even from non-acclimated seedlings, exhibited antifreeze activity. Interestingly, HrCHT1a and HrCHT1b purified from cold acclimated seedlings, exhibited 4 and 2 times higher antifreeze activities than those purified from non-acclimated seedlings, suggesting that antifreeze activity increased during cold acclimation. HrCHT1b exhibited 23-33% higher hydrolytic activity and 2-4 times lower antifreeze activity than HrCHT1a did. HrCHT1b was found to be a glycoprotein; however, its antifreeze activity was independent of glycosylation as even deglycosylated HrCHT1b exhibited antifreeze activity. Circular dichroism (CD) analysis showed that both these chitinases were rich in unusual β-stranded conformation (36-43%) and the content of β-strand increased (∼11%) during cold acclimation. Surprisingly, calcium decreased both the activities of HrCHT1b while in case of HrCHT1a, a decrease in the hydrolytic activity and enhancement in its antifreeze activity was observed. CD results showed that addition of calcium also increased the β-stranded conformation of HrCHT1a and HrCHT1b. This is the first report, which shows that antifreeze activity is constitutive property of class I chitinase and cold acclimation and calcium regulate these activities of chitinases by changing the secondary structure.

  9. USE OF SEA BUCKTHORN FRUITS IN THE PASTRY MANUFACTURING

    Directory of Open Access Journals (Sweden)

    RODICA A. STURZA

    2016-04-01

    Full Text Available Different researches on optimizing the shelf life of pastries (gingerbread, sponge cakes that are produced by adding sea buckthorn flour (2 - 4 % by weight of the flour used are presented in this study. This study shows the impact of biologically active substances on structural and mechanical, physicochemical, microbiological properties as well as the antioxidant activity of products under the conditions of in vitro gastric digestion. It has been demonstrated that the sea buckthorn flour increases the porosity of pastries, reduces the wet gluten amount and this contributes to moisture loss. The organoleptic assessment indicates that the addition of 2 % sea buckthorn flour improves the appearance, the color and the consistency of pastries. Microbiological analysis showed that samples with added sea buckthorn flour exhibit microbiological stability due to the sea buckthorn chemical composition. The antiradical activity DPPH• in conditions of in vitro gastric digestion of the samples with added sea buckthorn flour increases in a positive way, indicating a clearly positive effect on health.

  10. Abundance of active ingredients in sea-buckthorn oil.

    Science.gov (United States)

    Zielińska, Aleksandra; Nowak, Izabela

    2017-05-19

    Vegetable oils are obtained by mechanical extraction or cold pressing of various parts of plants, most often: seeds, fruits, and drupels. Chemically, these oils are compounds of the ester-linked glycerol and higher fatty acids with long aliphatic chain hydrocarbons (min. C14:0). Vegetable oils have a variety of properties, depending on their percentage of saturation. This article describes sea-buckthorn oil, which is extracted from the well characterized fruit and seeds of sea buckthorn. The plant has a large number of active ingredients the properties of which are successfully used in the cosmetic industry and in medicine. Valuable substances contained in sea-buckthorn oil play an important role in the proper functioning of the human body and give skin a beautiful and healthy appearance. A balanced composition of fatty acids give the number of vitamins or their range in this oil and explains its frequent use in cosmetic products for the care of dry, flaky or rapidly aging skin. Moreover, its unique unsaturated fatty acids, such as palmitooleic acid (omega-7) and gamma-linolenic acid (omega-6), give sea-buckthorn oil skin regeneration and repair properties. Sea-buckthorn oil also improves blood circulation, facilitates oxygenation of the skin, removes excess toxins from the body and easily penetrates through the epidermis. Because inside the skin the gamma-linolenic acid is converted to prostaglandins, sea-buckthorn oil protects against infections, prevents allergies, eliminates inflammation and inhibits the aging process. With close to 200 properties, sea-buckthorn oil is a valuable addition to health and beauty products.

  11. Healing effect of sea buckthorn, olive oil, and their mixture on full-thickness burn wounds.

    Science.gov (United States)

    Edraki, Mitra; Akbarzadeh, Armin; Hosseinzadeh, Massood; Tanideh, Nader; Salehi, Alireza; Koohi-Hosseinabadi, Omid

    2014-07-01

    The purpose of this study is to evaluate the healing effect of silver sulfadiazine (SSD), sea buckthorn, olive oil, and 5% sea buckthorn and olive oil mixture on full-thickness burn wounds with respect to both gross and histopathologic features. Full-thickness burns were induced on 60 rats; the rats were then were divided into 5 groups and treated with sea buckthorn, olive oil, a 5% sea buckthorn/olive oil mixture, SSD, and normal saline (control). They were observed for 28 days, and the wounds' healing process was evaluated. Wound contraction occurred faster in sea buckthorn, olive oil, and the sea buckthorn/olive oil mixture groups compared with the SSD and control groups. The volume of the exudates was controlled more effectively in wounds treated with the sea buckthorn/olive oil mixture. Purulent exudates were observed in the control group, but the others did not show infection. The group treated with sea buckthorn/olive oil mixture revealed more developed re-epithelialization with continuous basement membrane with a mature granulation tissue, whereas the SSD-treated group showed ulceration, necrosis, and immature granulation. The results show that sea buckthorn and olive oil individually are proper dressing for burn wounds and that they also show a synergetic effect when they are used together. A sea buckthorn and olive oil mixture could be considered as an alternative dressing for full-thickness burns because of improved wound healing characteristics and antibacterial property.

  12. Chemical Composition of Sea Buckthorn Leaves, Branches and Bark

    Directory of Open Access Journals (Sweden)

    Gradt Ina

    2017-06-01

    Full Text Available Sea buckthorn leaves and branches presently create waste-/by-products of harvesting after pruning the plants. It is already known that sea buckthorn berries are important for their chemical composition and based on this occupy a wide field in nutrition. We raised the idea that sea buckthorn leaves, branches, and especially the bark, have also an extraordinary chemical composition like the berries. The aim of this study was to describe these by-products. For this purpose, detailed full analyses of corresponding samples from Russia (seven varieties and Germany (four varieties were performed. Especially the dry mass, fat content, proteins, carbohydrates, starch content, and crude fiber were investigated to obtain an overview. Minor components like total phenol content, metals, and water- and fat-soluble vitamins were also studied. All analytical parameters were based on an official collection of analysis methods (German ASU - amtliche Sammlung von Untersuchungsverfahren. The results of the full analysis of leaves and branches show some interesting aspects about the differences between male and female plants. Furthermore, we observed differences between Russian and German sea buckthorn varieties. Investigation of minor components showed that vitamins were present in very low amount (< 0.1 %.

  13. Pre-logging Treatment of Invasive Glossy Buckthorn (Frangula alnus Mill. Promotes Regeneration of Eastern White Pine (Pinus strobus L.

    Directory of Open Access Journals (Sweden)

    Thomas D. Lee

    2016-12-01

    Full Text Available Non-native glossy buckthorn (Frangula alnus Mill. is invasive in forests of the northeastern USA but little is known of its effects on tree regeneration. We tested whether killing buckthorn stems before logging reduces its post-logging abundance and increases the density and height of eastern white pine (Pinus strobus L. seedlings. Three 0.4 ha plots were clearcut, three were thinned, and three were left as controls. Each plot had previously been divided into three subplots that received different buckthorn treatments during the two years before logging. Buckthorn treatments were (1 stems cut at base five times; (2 stems cut once then heat killed four times; (3 untreated control. Three years post-logging, buckthorn density and stem height were unaffected by logging but equally reduced by the two buckthorn treatments. Buckthorn reduction increased density and height of pine seedlings, and seedling height also increased with logging. In the fifth year post-logging, pine height growth and biomass were greater in clearcut than in thinned treatments, greater in areas of buckthorn removal and, within treated subplots, greater in areas with low buckthorn density than in thickets of recovering buckthorn. Thus, although buckthorn inhibited regenerating pine, pre-logging destruction of buckthorn stems reduced such competition for at least four years.

  14. Generalized yellow skin caused by high intake of sea buckthorn.

    Science.gov (United States)

    Grad, Simona Costin; Muresan, Iulia; Dumitrascu, Dan L

    2012-01-01

    One of the reasons many people use sea buckthorn is that it contains several antioxidants such as vitamin C, vitamin E, carotenoids, fatty acids, organic acids, and flavonoids. These substances promote proper cellular function and health which is expected to lead to or maintain beautiful hair, skin and nails, and also have several potential applications in cancer therapy, cardiovascular diseases, gastric ulcers, and liver cirrhosis. We present the case of a 45-year-old male with yellow staining of the skin that had appeared gradually over the last 3 months. After a thorough check-up and the exclusion of any other etiology, we found out that the patient's skin color was due to reported chronic consumption of sea buckthorn for the last 6 months. This overdose had initially remained undisclosed because the patient considered it not relevant for the history of the disease. This case emphasizes the role of taking a thorough medical history of patients with yellow skin in order to allow a correct differential diagnosis. Overdose of alternative therapies like sea buckthorn should be considered in cases of yellow skin with an obscure etiology. Copyright © 2012 S. Karger AG, Basel.

  15. Protective effects of a novel sea buckthorn wine on oxidative stress and hypercholesterolemia.

    Science.gov (United States)

    Negi, Bharti; Kaur, Rajdeep; Dey, Gargi

    2013-02-01

    We developed a novel sea buckthorn wine containing significant in vitro free radical-scavenging activity. High-performance liquid chromatographic analysis of the sea buckthorn wine revealed that it contains high rutin, myricetin and quercetin levels compared to Cabernet Shiraz wine. In this study, we evaluated the protective effects of sea buckthorn wine against phorone-induced oxidative stress and high-cholesterol diet induced hypercholesterolemia in male LACA mice. Oral administration of sea buckthorn wine increased the redox ratio accompanied by reduction of oxidized glutathione levels leading to attenuation of phorone-induced oxidative stress. Furthermore, the sea buckthorn wine supplementation reduced hepatic lipid peroxidation and increased the superoxide dismutase activity indicating improved resistance to oxidative stress. In addition, high-cholesterol-fed mice administered with sea buckthorn wine exhibited a 197% increase in the HDL-C/LDL-C ratio compared to high-cholesterol diet treated mice. These studies provide important evidence that sea buckthorn wine exerts protective effects against oxidative stress and hypercholesterolemia.

  16. Exotic Invasive Shrub Glossy Buckthorn Reduces Restoration Potential for Native Forest Herbs

    Directory of Open Access Journals (Sweden)

    Caroline Hamelin

    2017-02-01

    Full Text Available Invasive glossy buckthorn could reduce restoration potential for understory native forest herbs by compromising their growth and biodiversity. Few studies of glossy buckthorn’s effects on forest herbs exist, and none were done in early-successional, partially open hardwood forests. This study was conducted in a mature hybrid poplar plantation invaded by buckthorn, located in southeastern Québec. We tested the effect of buckthorn removal on the growth of three forest herb species, whether this effect varied among species, and if canopy type (two poplar clones influenced this effect. Forest herbs were planted in herbicide (buckthorn removed and control treatments in the plantation understory, an environment similar to that of early-successional hardwood forests. Over the first two growing seasons, species showed specific reactions to buckthorn cover. Mean relative growth rate (RGR for Asarum canadense and Polygonatum pubescens was increased in the herbicide treatment (48% and 33%, respectively and decreased in the control treatment (−35% and −33%, respectively. Sanguinaria canadensis growth was the highest among species, with no difference between treatments. No effects of canopy type were detected. Results suggest that planting forest herbs for restoration purposes may be unsuccessful if buckthorn is present. Important changes in understory flora biodiversity are likely to occur over the long term in forests invaded by buckthorn.

  17. [Study on three different species tibetan medicine sea buckthorn by 1H-NMR-based metabonomics].

    Science.gov (United States)

    Su, Yong-Wen; Tan, Er; Zhang, Jing; You, Jia-Li; Liu, Yue; Liu, Chuan; Zhou, Xiang-Dong; Zhang, Yi

    2014-11-01

    The 1H-NMR fingerprints of three different species tibetan medicine sea buckthorn were established by 1H-HMR metabolomics to find out different motablism which could provide a new method for the quality evaluation of sea buckthorn. The obtained free induction decay (FID) signal will be imported into MestReNova software and into divide segments. The data will be normalized and processed by principal component analysis and.partial least squares discriminant analysis to perform pattern recognition. The results showed that 25 metabolites belonging to different chemical types were detected from sea buckthorn,including flavonoids, triterpenoids, amino acids, carbohydrates, fatty acids, etc. PCA and PLS-DA analysis showed three different varietiest of sea buckthorn that can be clearly separated by the content of L-quebrachitol, malic acid and some unidentified sugars, which can be used as the differences metabolites of three species of sea buckthorn. 1H-NMR-based metabonomies method had a holistic characteristic with sample preparation and handling. The results of this study can offer an important reference for the species identification and quality control of sea buckthorn.

  18. Potential cardiovascular implications of Sea Buckthorn berry consumption in humans.

    Science.gov (United States)

    Sayegh, Marietta; Miglio, Cristiana; Ray, Sumantra

    2014-08-01

    Diets rich in fruits and vegetables have been correlated with decreased risks of cardiovascular disease. Particularly, berry consumption has been associated with reductions in cardiovascular risk. Despite the range of potentially beneficial phytochemical components (vitamins, polyphenols, carotenoids, and fatty acids), there is little evidence underpinning the cardiovascular effects of sea buckthorn (SB) berries. The purpose of this review is to evaluate the benefits of SB consumption on cardiovascular health in human trials. Only six human studies were found, which examine the effect of SB berries on cardiovascular outcomes (i.e., lipid metabolism, platelet aggregation, and inflammation). Although there appears to be an inverse association between SB consumption and cardiovascular risk factors, the evidence is still scarce and the results are inconsistent. In addition, limitations in study design made it difficult to form firm conclusions. More "high-quality" human clinical trials are needed in order to establish the cardio-protective benefits of SB berries.

  19. Identification of urinary biomarkers after consumption of sea buckthorn and strawberry, by untargeted LC-MS metabolomics

    DEFF Research Database (Denmark)

    Cuparencu, Catalina Sinziana; Andersen, Maj-Britt Schmidt; Gürdeniz, Gözde

    2016-01-01

    , strawberry and sea buckthorn, in humans. A randomized controlled single-blinded three-way cross-over meal study was conducted in 16 overweight men. The intervention meals consisted of sea buckthorn puree, strawberry puree or an iso-caloric control drink. Urine samples were collected on each test day at t...

  20. Sea buckthorn decreases and delays insulin response and improves glycaemic profile following a sucrose-containing berry meal: a randomised, controlled, crossover study of Danish sea buckthorn and strawberries in overweight and obese male subjects.

    Science.gov (United States)

    Mortensen, Maria Wichmann; Spagner, Camilla; Cuparencu, Cătălina; Astrup, Arne; Raben, Anne; Dragsted, Lars Ove

    2017-10-11

    Berries and mixed berry products exert acute effects on postprandial glycaemia and insulinemia, but very few berries have been studied, and primarily in normal weight subjects. Sea buckthorn and strawberry are compositionally widely different berries and may likely produce different responses. The effects of strawberry and sea buckthorn on postprandial glycaemia and insulinemia were examined in overweight or obese male subjects. Subjective appetite sensations and ad libitum intake were also examined. The study was conducted as a randomised, controlled, single-blinded, three-way crossover study. Eighteen subjects were studied in three 2-h meal tests followed by a subsequent ad libitum meal. Test meals contained added sucrose and either sea buckthorn, strawberry or no berries with added fructose (control). Blood samples were collected at t = 0, 30, 45, 60, 90 and 120 min. Subjective appetite sensations were recorded at t = 0, 15, 30, 45, 60, 90, 120, and 140 min and subsequent ad libitum intake was recorded. Statistical differences in all continuous measures were evaluated based on the existence of a meal or a time-meal interaction by repeated measures linear model analyses or by differences in AUC by linear mixed models. None of the berries affected postprandial glucose. However, sea buckthorn improved glycaemic profile (44.7%, p Sea buckthorn also resulted in a decrease in plasma insulin concentration at 30 min (39.6%, p sea buckthorn compared with control (23.6%, p sea buckthorn meal compared to control. There was no effect on postprandial glucose response to a sugar challenge given together with purees of strawberry or sea buckthorn. Sea buckthorn decreased and delayed the insulin response and improved glycaemic profile compared with control. Strawberry had no such effects. No important differences were seen for the appetite measures. Sea buckthorn might be useful as a culinary tool for lowering meal insulin response.

  1. Effect of sea buckthorn protein on the intestinal microbial community in streptozotocin-induced diabetic mice.

    Science.gov (United States)

    Yuan, Huaibo; Shi, Fangfang; Meng, Lina; Wang, Wenjuan

    2018-02-01

    This study investigated the intestinal microbial community distribution of Type 2 diabetic mice and discussed the effects of the sea buckthorn protein on the regulation of gut microbes. Date was collected for 12 cases of normal mice (NC group), 12 cases of Type 2 diabetic mice (DC group), and 12 cases of highly concentrated sea buckthorn seed protein dosed mice (SSPH group). This study analysed fecal samples, measured faecal pH value, and cultivated and determined intestinal bacteria count. This investigation also included the extraction of faecal samples for genomic DNA, PCR amplification of bacterial V3 16S rDNA products by denaturing gradient gel electrophoresis, DGGE map analysis of intestinal flora, determination of intestinal bacteria richness, Shannon-Wiener index and evenness index, and image similarity cluster analysis with UPGMA clustering. This study analysed and elucidated differences between the normal mice group, diabetic mice group, and sea buckthorn protein supplemented group, and the structures of respective intestinal flora. The mice supplemented with sea buckthorn protein exhibited an obvious drop in body weight and blood glucose levels. The Bifidobacterium, Lactobacillus, Bacteroides, and Clostridium coccoides populations recovered. The amplification of the 16S rDNA gene V3 region revealed that the species of intestinal microbes in the treatment group were adjusted to a certain extent. Analysis by ARDRA confirmed that sea buckthorn protein could increase type 2 diabetes in mice intestinal microorganism diversity (H) and simpson (E). Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Sea buckthorn seed oil protects against the oxidative stress produced by thermally oxidized lipids.

    Science.gov (United States)

    Zeb, Alam; Ullah, Sana

    2015-11-01

    Thermally oxidized vegetable ghee was fed to the rabbits for 14 days with specific doses of sea buckthorn seed oil (SO). The ghee and SO were characterized for quality parameters and fatty acid composition using GC-MS. Rabbits serum lipid profile, hematology and histology were investigated. Major fatty acids were palmitic acid (44%) and oleic acid (46%) in ghee, while SO contains oleic acid (56.4%) and linoleic acid (18.7%). Results showed that oxidized vegetable ghee increases the serum total cholesterol, LDL-cholesterols, triglycerides and decrease the serum glucose. Oxidized ghee produced toxic effects in the liver and hematological parameters. Sea buckthorn oil supplementation significantly lowered the serum LDL-cholesterols, triglycerides and increased serum glucose and body weight of the animals. Sea buckthorn oil was found to reduce the toxic effects and degenerative changes in the liver and thus provides protection against the thermally oxidized lipids induced oxidative stress. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Enzymatic technology for Sea buckthorn oil extraction and its biochemical analysis

    Directory of Open Access Journals (Sweden)

    D Munkhbayar

    2014-12-01

    Full Text Available A research was conducted  mix five Sea buckthorn berries harvested Mongolian Central Zone, and an integrated processing composed of an enzymatic unit operation and pulp oil and raw juice have been produced by centrifugal technology. For the extraction of Sea buckthorn oil, pectinase were used simultaneously hydrolyze Sea buckthorn berry pulp. In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and raw juice from Sea buckthorn berry: Pectinase. Pectinase dose of 0.5 %, extraction time 120 minutes, hydrolysis temperature 55°C were found optimum, and maximum oilyieldof 5.0 - 5.1 % was achieved under these conditions, and the recovery of the optimized extraction process was calculated to 95.7 – 98.0 % based on 5.2 %theoreticaloil content of sea buckthorn fruit. In addition, physical and biochemical compositions of Sea buckthorn pulp oil was analyzed modern methods as HPLC, Spectrophotometer, Gravimeter, Pycnometer and Refractometers. Fatty acids by HPLC were identified in Pulp oil, mainly including 42,5% palmitoleic acid, 11.2 % linoleic acid, 1.2 % linolenic acid, 12.3% oleic acid, and unsaturated fatty acids occupied 70.9% of the total fatty acids. In conclusion, the enzymatic extraction process has thebenefitsof mild conditions, safe, fast for oil extraction, high yield, and pure juice after oil extraction.DOI: http://doi.dx.org/10.5564/mjc.v15i0.325 Mongolian Journal of Chemistry 15 (41, 2014, p62-65

  4. Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry.

    Science.gov (United States)

    He, Caiyun; Zhang, Guoyun; Zhang, Jianguo; Zeng, Yanfei; Liu, Juanjuan

    2017-05-01

    Berries of sea buckthorn, known as the "king of vitamin C," are abundant in antioxidants, have attractive colors, and are an excellent material with which to study the relationships between berry color, antioxidants, and berry quality. No study has yet determined the molecular basis of the relationship between sea buckhorn berries and their color and antioxidant levels. By using RNA-seq, LC-MS/MS, and LC/GC-MS technology and selecting red (darkest colored) and yellow (lightest colored) sea buckthorn berries at different development stages, this study showed that the red and yellow berry resulted from a higher ratio of lycopene to β-carotene and of β-carotene to lycopene content, respectively. The uronic acid pathway-a known animal pathway-in ascorbic acid synthesis was found in sea buckthorn berries, and the higher expression of UDP-glucuronosyltransferase in red berries was consistent with the higher content of ascorbic acid. In summary, multiomic data showed that the color of sea buckthorn berries is mainly determined by β-carotene and lycopene; red sea buckthorn berries were richer than yellow berries in antioxidants, such as carotenoids, flavonoids, and ascorbic acid; and the animal pathway might be operating in sea buckthorn.-He, C., Zhang, G., Zhang, J., Zeng, Y., Liu, J. Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry. © FASEB.

  5. THE INFLUENCE OF HEIGHT ABOVE SEA LEVEL ON WATER CONDUCTING TISSUE OF SEA BUCKTHORN

    Directory of Open Access Journals (Sweden)

    M. U. Umarov

    2013-01-01

    Full Text Available The article gives the anatomical description of sea buckthorn, that grows 1200 and 1600 above the sea level in Eastern Caucasus. It analyses quantitative changes of its traits - radial growth, elements of water conducting tissue and the degree of its variability.

  6. Parasitism of Autumnal Morphs of the Soybean Aphid (Hemiptera: Aphididae) by Binodoxys communis (Hymenoptera: Braconidae) on Buckthorn

    National Research Council Canada - National Science Library

    Mark K. Asplen; Kris A. G. Wyckhuys; George E. Heimpel

    2011-01-01

    ... to A. glycines throughout the aphid life cycle and follow it to its primary host; and, if it does, 2) is parasitoid migration facilitated by phoretic movement within buckthorn-specific winged aphids...

  7. The content determination of calcium, magnesium and ascorbic acid in sea buckthorn fruits at vulcan coal dump

    Directory of Open Access Journals (Sweden)

    Andreea BRASOVAN

    2009-11-01

    Full Text Available The study presents data on content determination of calcium, magnesium and ascorbic acid in sea buckthorn fruits collected from Vulcan coal dump. The dump was never covered with soil or fertilizers containing azoth, potassium and phosphorus. The sea buckthorn was planted directly on the sterile and it has an important function in stabilizing the acclivity and fixing the atmospheric azoth with the nodosities on the roots. So, the sea buckthorn becomes widespread plant in coal dumps because it helps to the soil formation. The results obtained after the determination of calcium, magnesium and ascorbic acid from the fruits of this plant were compared with the values already reported in various references, concluding that these differences are insignificant (3.7% for magnesium and 9.4% for calcium. Therefore, the sea buckthorn from the dump can be used in food industry, as well as in pharmaceutics and medicine.

  8. Female plants of Hippophae salicifolia D. Don are more responsive to cold stress than male plants

    OpenAIRE

    Gupta, Sanjay Mohan; Grover, Atul; Pandey, Pankaj; Ahmed, Zakwan

    2012-01-01

    Hippophae salicifolia D. Don (Seabuckthorn) grows in stressful environment of high altitude under conditions of low temperature and low availability of water. We have studied gender based differences in physiochemical response to cold stress in male and female plants of Seabuckthorn. After 24 h of cold stress about 32 and 66 % higher electrolyte leakage (EL) was recorded in male and female plants respectively. Relative water content (RWC) at the end of 24 h stress was higher in male plants (~...

  9. A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers.

    Science.gov (United States)

    Yang, G; Ding, J; Wu, L R; Duan, Y D; Li, A Y; Shan, J Y; Wu, Y X

    2015-03-13

    DNA fingerprinting is both a popular and important technique with several advantages in plant cultivar identification. However, this technique has not been used widely and efficiently in practical plant identification because the analysis and recording of data generated from fingerprinting and genotyping are tedious and difficult. We developed a novel approach known as a cultivar identification diagram (CID) strategy that uses DNA markers to separate plant individuals in a more efficient, practical, and referable manner. A CID was manually constructed and a polymorphic marker was generated from each polymerase chain reaction for sample separation. In this study, 67 important sea buckthorn cultivars cultivated in China were successfully separated with random amplified polymorphic DNA markers using the CID analysis strategy, with only seven 11-nucleotide primers employed. The utilization of the CID of these 67 sea buckthorn cultivars was verified by identifying 2 randomly chosen groups of cultivars among the 67 cultivars. The main advantages of this identification strategy include fewer primers used and separation of all cultivars using the corresponding primers. This sea buckthorn CID was able to separate any sea buckthorn cultivars among the 67 studied, which is useful for sea buckthorn cultivar identification, cultivar-right-protection, and for the sea buckthorn nursery industry in China.

  10. The effect of a diet supplemented with sea-buckthorn pomace on the colour and viscosity of the egg yolk

    Directory of Open Access Journals (Sweden)

    Petr Dvořák

    2017-01-01

    Full Text Available Sea-buckthorn pomace is a very valuable product which contains not only important nutrients but also high-quality oils. The question addressed in the present study was to what extent the diet containing the sea-buckthorn pomace would affect the viscosity and colour of egg yolk measured in the CIELAB system. The feeding mixture for laying hens was supplemented with 20; 50 and 100 g∙kg-1 of sea-buckthorn pomace. As a result, colour indices of the egg yolk such as L*, a* and b* changed significantly (α = 0.01. The greatest relative enhancement was observed for indicator a* for the red colour. Visually, this corresponds to the more intense orange colour of the egg yolk. The addition of sea-buckthorn pomace to the diet for laying hens resulted in a larger increase in indicator ∆E* (CIE total colour difference compared to the control group. Colour indicator hab is the only indicator whose value oscillated around that determined for the control group. The addition of sea-buckthorn pomace to the diet resulted in an increase in colour indices a*, b* and C*ab. Indicator ∆E* also increased significantly with an increasing amount of sea-buckthorn pomace in a diet. Egg yolks were darker, had more intense red and yellow colours, and showed lower viscosity which are all features preferred by the consumer.

  11. Evaluation of Probiotic L. rhamnosus GG as a Protective Culture in Sea Buckthorn-Based Beverage

    Directory of Open Access Journals (Sweden)

    Srijita Sireswar

    2017-10-01

    Full Text Available The main objective of this paper was to evaluate the efficiency of probiotic strain, Lactobacillus rhamnosus GG (ATCC 53103 as a protective culture when present in a food system. A non-fermented sea buckthorn-based beverage was developed. To meet the required criteria for probiotic beverage (viable count of 8 log CFU mL−1, the acidic juice had to be supplemented with whey protein concentrate (WPC. The obtained beverage had a shelf life of two weeks. Furthermore, the inhibitory potential of Lactobacillus-fortified-WPC-supplemented juice matrix was evaluated against E. coli (ATCC 25922 which is a major agent responsible for food contamination and shelf spoilage. Results indicated that the fortification of beverage with L. rhamnosus GG appeared to create an effective hurdle for multiplication of E. coli in the sea buckthorn-WPC system.

  12. Protection against vascular endothelial dysfunction by polyphenols in sea buckthorn berries in rats with hyperlipidemia.

    Science.gov (United States)

    Yang, Fang; Suo, Yourui; Chen, Dongli; Tong, Li

    2016-07-19

    Chronic hyperlipemia increases the incidence of vascular endothelial dysfunction and can even induce cardiovascular disease. Sea buckthorn contains a host of bioactives such as flavonoids and polyphenols that can prevent the development of cardiovascular disease. The current study isolated active ingredients, polyphenols, from sea buckthorn berries (SVP) and orally administered SVP at a dose of 7-28 mg/kg. This treatment significantly reduced serum lipids, it enhanced the activity of antioxidant enzymes, and it decreased the level of serum TNF-α and IL-6. SVP also alleviate vascular impairment by decreasing the expression of eNOS, ICAM-1, and LOX-1 mRNA and proteins in aortas of rats with hyperlipidemia. Based on these findings, SVP has antioxidant action and it protects endothelium.

  13. Advances in improvement of quality and resistance in a multipurpose crop: sea buckthorn.

    Science.gov (United States)

    Ruan, Cheng-Jiang; Rumpunen, Kimmo; Nybom, Hilde

    2013-06-01

    Sea buckthorn is a berry crop with multiple uses. The berries are highly appreciated for their unique taste but are also very rich in bioactive compounds with powerful nutritional and medicinal values. In addition, the plants grow well under adverse conditions, and are often used to fight soil erosion. Utilization of sea buckthorn has therefore increased around the world but serious problems have, nevertheless, been encountered due to drought, salinity, diseases and insect pests. This review covers important aspects of sea buckthorn research, such as heritable and environmentally induced variation in biochemical compounds, causes and effects of the devastating dried-shrink disease, susceptibility to insect pests, methods for conventional breeding, and the utilization of DNA markers for taxonomical and population genetic analyses, and for investigating the inheritance of quality and resistance traits. We also present possibilities to implement innovative biotechnological breeding methods, especially metabolite profiling and MAS/GRC-based markers, for fast and efficient development of elite genotypes with specific nutritional- and health-related bioactive compounds and strong resistance to biotic and abiotic stress.

  14. Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei.

    Science.gov (United States)

    Pop, Oana Lelia; Dulf, Francisc Vasile; Cuibus, Lucian; Castro-Giráldez, Marta; Fito, Pedro J; Vodnar, Dan Cristian; Coman, Cristina; Socaciu, Carmen; Suharoschi, Ramona

    2017-11-24

    Probiotics are bacteria that can provide health benefits to consumers and are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heat treatment and with an in vitro gastrointestinal model. The characterization of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis), high-performance liquid chromatography photodiode array detection method (HPLC-PDA), gas chromatography coupled with mass spectrometry (GS-MS) and Cryo scanning electron microscopy (Cryo-SEM). During heat treatment, the entrapped probiotic cells proved high viability (>6 CFU log/g), even at temperatures above 50 °C. The rich in monounsaturated fatty acids sea buckthorn fraction improved the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15% higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this approach may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.

  15. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.

    Science.gov (United States)

    Wang, Yi; Nie, Fangyuan; Ouyang, Jian; Wang, Xiaoyan; Ma, Xiaofeng

    2014-10-01

    Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 μg/ml. 3-4,5-Dimethylthiazol-2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 μg/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.

  16. Effect of sea buckthorn and vitamin E on growth performance of Acipenser stellatus (Pallas 1771 juveniles

    Directory of Open Access Journals (Sweden)

    Oana Georgiana Dorojan

    2015-05-01

    Full Text Available Addition of natural additives (prebiotics, probiotics, phytobiotics in fish feed is a benefic method because it leads to improved growth performance of fish reared in intensive production systems. Sea buckthorn is one of the most commonly phytobiotics used in aquaculture because of its antioxidant, antimicrobial, immunostimulant, anti-stress, cytoprotective, liver and tissue regeneration properties. Sea buckthorn berries are rich in vitamins (B, C, E and K, flavonoids, carotenoids, tocopherols and many volatile compounds. In this context the aim of this study was to evaluate the effects of dietary vitamin E (500 mg/kg diet and sea buckthorn on growth performance and survival of the A. stellatus juveniles reared in a recirculating aquaculture system. The experiment took place in the recirculating pilot system of “Dunărea de Jos” University of Galați. In order to emphasize the influence of the above mentioned immunostimulants on sevruga fingerlings with different genetic background, four experimental groups from different genitors (V1: ♀2 Danube x ♂1 Aquaculture, V2: ♀1 Danube x ♂1 Danube, V3: ♀1 Danube x ♂2 Aquaculture and V4: ♀2 Danube x ♂2 Danube have been used. At the end of the experiment no significant differences (p>0.05 were observed in terms of final mean weight, individual growth rate, SGR, FCR, PER, between the sevruga fingerlings obtained from wild genitors, comparing with those obtained from aquaculture genitors.

  17. Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Oana Lelia Pop

    2017-11-01

    Full Text Available Probiotics are bacteria that can provide health benefits to consumers and are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heat treatment and with an in vitro gastrointestinal model. The characterization of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis, high-performance liquid chromatography photodiode array detection method (HPLC-PDA, gas chromatography coupled with mass spectrometry (GS-MS and Cryo scanning electron microscopy (Cryo-SEM. During heat treatment, the entrapped probiotic cells proved high viability (>6 CFU log/g, even at temperatures above 50 °C. The rich in monounsaturated fatty acids sea buckthorn fraction improved the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15% higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this approach may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.

  18. Effects of sea buckthorn berries on infections and inflammation: a double-blind, randomized, placebo-controlled trial.

    Science.gov (United States)

    Larmo, P; Alin, J; Salminen, E; Kallio, H; Tahvonen, R

    2008-09-01

    To study the effect of sea buckthorn berries on the number and duration of common cold (CC) infections. As secondary objectives the effects on digestive and urinary tract infections (DTI, UTI), and serum C-reactive protein (CRP) concentrations were also investigated. A total of 254 healthy volunteers were randomly assigned to receive sea buckthorn or placebo product during the study, which 233 of them completed. There were no significant differences in the number or duration of CC or DTI between groups (CC: relative risks (sea buckthorn vs placebo) for the number and duration were 1.15 (95% CI 0.90-1.48) and 1.05 (95% CI 0.87-1.27), respectively). In the sea buckthorn group, as compared to the placebo, the serum CRP concentrations decreased significantly (difference in median change -0.059 mg/l, P=0.039). The number of UTI was too small to draw solid conclusions, but the results indicate the subject merits further investigation. Sea buckthorn berries did not prevent CC or DTI. However, a reductive effect on CRP, a marker of inflammation, and a risk factor for cardiovascular diseases, was detected.

  19. Above-ground biomass models for Seabuckthorn (Hippophae salicifolia) in Mustang District, Nepal

    DEFF Research Database (Denmark)

    Rajchal, Rajesh; Meilby, Henrik

    2013-01-01

    Seabuckthorn (Hippophae salicifolia D. Don.), an important multi-purpose tree, is found at altitudes of 2000–3600 m amsl in Nepal, but so far no models have been developed for estimating the biomass of this species, thus hampering resource assessment and management planning. Hence, the objective...... of this study was to develop local biomass models for wood, fruit, and leaves of Seabuckthorn. In November 2006, a diameter-stratified sample of 30 trees was harvested in Lete and Kunjo Village Development Committees at an altitude of about 2300 m amsl in the lower part of Mustang District, Nepal. The fresh...... weight of fruit and oven-dry weight of wood (stem and branches) and leaves were measured and used as a basis for developing biomass models. Diameters of the trees were measured at 30 cm above ground whereas the heights were measured in terms of the total tree height (m). Among several models tested...

  20. Sea buckthorn decreases and delays insulin response and improves glycaemic profile following a sucrose-containing berry meal

    DEFF Research Database (Denmark)

    Mortensen, Maria Wichmann; Spagner, Camilla; Cuparencu, Cătălina

    2017-01-01

    PURPOSE: Berries and mixed berry products exert acute effects on postprandial glycaemia and insulinemia, but very few berries have been studied, and primarily in normal weight subjects. Sea buckthorn and strawberry are compositionally widely different berries and may likely produce different...... responses. The effects of strawberry and sea buckthorn on postprandial glycaemia and insulinemia were examined in overweight or obese male subjects. Subjective appetite sensations and ad libitum intake were also examined. METHODS: The study was conducted as a randomised, controlled, single-blinded, three......-way crossover study. Eighteen subjects were studied in three 2-h meal tests followed by a subsequent ad libitum meal. Test meals contained added sucrose and either sea buckthorn, strawberry or no berries with added fructose (control). Blood samples were collected at t = 0, 30, 45, 60, 90 and 120 min. Subjective...

  1. The nutritional value of leaves of selected berry species

    Directory of Open Access Journals (Sweden)

    Wioletta Biel

    Full Text Available ABSTRACT: Although the medicinal properties of berry fruit are well known, there is relatively little information available concerning the applications of other parts of berry plants. Thus, in this study we determined the nutritional value of the leaves of selected berry species and their possible application in health promoting diets. The levels of nutrients, and macro- and microelements in the leaves of four species collected from allotment gardens in the city of Szczecin, Poland (53°26′17″ N, 14°32′32″ E; altitude 7 m a.s.l. were identified: raspberry (Rubus idaeus L., blackberry (Rubus fruticosus L., chokeberry (Aronia melanocarpa L., and sea buckthorn (Hippophae rhamnoides L.. Sea buckthorn leaves were the richest source of protein, raspberry leaves had the highest levels of lipids, and the leaves of all four species studied were a rich source of crude fibre and dietary fibre fractions. Desirable Ca:P and Na:K ratios indicated their potential as a good source of minerals essential to bone formation and the treatment of hypertension. Sea buckthorn leaves contained high but also safe Fe levels (within recommended WHO limits and, therefore, may become an alternative rich source of this element.

  2. Evaluation of Probiotic L. rhamnosus GG as a Protective Culture in Sea Buckthorn-Based Beverage

    OpenAIRE

    Srijita Sireswar; Gargi Dey; Kinjoll Dey; Arkasish Kundu

    2017-01-01

    The main objective of this paper was to evaluate the efficiency of probiotic strain, Lactobacillus rhamnosus GG (ATCC 53103) as a protective culture when present in a food system. A non-fermented sea buckthorn-based beverage was developed. To meet the required criteria for probiotic beverage (viable count of 8 log CFU mL−1), the acidic juice had to be supplemented with whey protein concentrate (WPC). The obtained beverage had a shelf life of two weeks. Furthermore, the inhibitory potential of...

  3. Effect of modified atmosphere packaging (map) on the quality of sea buckthorn berry fruits during postharvest storage

    Science.gov (United States)

    The effect of modified atmosphere packaging (MAP) on the quality of the berry fruits of sea buckthorn (SBT) during refrigerated storage was investigated. SBT berries were packaged in 160 and 525 oxygen transmission rate (OTR) films or in vented clamshell containers (air control) and stored at 10C fo...

  4. Structure of the style and pollen tube pathway in the Ziziphoid and Rhamnoid clades of Rhamnaceae.

    Science.gov (United States)

    Gotelli, Marina M; Galati, Beatriz G; Zarlavsky, Gabriela; Medan, Diego

    2017-09-14

    The ultrastructure of the style and pollen tube pathway before, during and after anthesis were studied in 13 species belonging to the tribes Pomaderreae, Paliureae, Colletieae and Gouanieae (Ziziphoid clade) and Rhamneae (Rhamnoid clade) using light microscopy and transmission electron microscopy. The aim of this study is to provide new morphological characters useful for phylogenetic analysis at suprageneric level in Rhamnaceae. The patterns of pollen tube growth and the ultrastructural changes undergone by cells of the style were also described. Species of Rhamneae (Scutia buxifolia and Condalia buxifolia) have a solid style, with the transmitting tissue forming three independent strands (S. buxifolia) or a central, single horseshoe-shaped strand as seen in transversal section (C. buxifolia) which could derive from the fusion of formerly independent strands. In contrast, Pomaderreae, Gouanieae and Paliureae showed semi-solid styles, while in Colletieae, as previously reported, the style is hollow with two or three stylar canals. The style anatomy and the ultrastructure of the pollen tube pathway show that there is a tendency towards a solid style with a single strand of transmitting tissue within the family. The three-canalled hollow style could be the plesiomorphic state of the character "type of style" in the family, the semi-solid style the synapomorphic state and the solid style with three strands of transmitting tissue the apomorphic state, with the solid style with a single strand of transmitting tissue as the most derived state. Therefore, Colletieae would be the most basal tribe of the Ziziphoid clade.

  5. A novel probiotic fermented beverage based on soymilk and sea buckthorn syrup

    Directory of Open Access Journals (Sweden)

    Nicoleta Maricica Maftei

    2012-08-01

    Full Text Available In the last decade, there is an increasing interest in using nondairyingredients as substrates for certain strains of bifidobacteria to deliver the benefits of probiotics to a wider group of consumers. This research aimed to explore the use of soymilk and sea buckthorn syrup as substrates for bifidobacteria fermentation. Microbial population, pH, and titratable acidity were measured during the fermentation period while the viability, pH, titratable acidity and water holding capacity were determined during the storage time at 4°C ± 1°C within 14 days. Survival and stability of Bifidobacterium bifidus (Bb-12®, Bb inoculated into a beverage when exposed to simulated gastrointestinal tract conditions, were assessed. TheBb-12® strain exhibited the highest viable cell numbers when exposed to simulated gastrointestinal tract conditions.

  6. Studies on binding mechanism between carotenoids from sea buckthorn and thermally treated α-lactalbumin

    Science.gov (United States)

    Dumitraşcu, Loredana; Ursache, Florentina Mihaela; Stănciuc, Nicoleta; Aprodu, Iuliana

    2016-12-01

    Sea buckthorn is a natural food ingredient rich in bioactive compounds such as carotenoids, tocopherols, sterols, flavonoids, lipids, vitamins, tannins and minerals. Herein, fluorescence and UV-vis techniques were used to study the interaction of heat treated α-lactalbumin (α-LA) with carotenoids from sea buckthorn berries extract (CSB) and β-carotene. Further atomic level details on the interaction between α-LA and β-carotene were obtained by means of molecular modelling techniques. The quenching rate constants, binding constants, and number of binding sites were calculated in the presence of CSB. The emission spectral studies revealed that, CSB have the ability to bind α-LA and form a ground state complex via static quenching process. Maximum degree of quenching was reached at 100 °C, where β-carotene and CSB quenched the Trp fluorescence of α-LA by 56% and 47%, respectively. In order to reveal the interaction between CSB and α-LA, the thermodynamic parameters were determined from the van't Hoff plot based on the temperature dependence of the binding constant. In agreement with the in silico observations, the thermodynamic parameters enabled us to consider that the association between α-LA and β-carotene is a spontaneous process driven by enthalpy, dominated mainly by the van der Waals interaction, but hydrophobic interactions might also be considered. The interaction between CSB and α-LA was further confirmed by UV-vis absorption spectra, where a blue shift of position was noticed at higher temperature suggesting the complex formation. The results provided here supply a better understanding of the binding of CSB to α-LA, which can be further exploited in designing new healthy food applications.

  7. Comparative de novo transcriptome analysis of male and female Sea buckthorn.

    Science.gov (United States)

    Bansal, Ankush; Salaria, Mehul; Sharma, Tashil; Stobdan, Tsering; Kant, Anil

    2018-02-01

    Sea buckthorn is a dioecious medicinal plant found at high altitude. The plant has both male and female reproductive organs in separate individuals. In this article, whole transcriptome de novo assemblies of male and female flower bud samples were carried out using Illumina NextSeq 500 platform to determine the role of the genes involved in sex determination. Moreover, genes with differential expression in male and female transcriptomes were identified to understand the underlying sex determination mechanism. The current study showed 63,904 and 62,272 coding sequences (CDS) in female and male transcriptome data sets, respectively. 16,831 common CDS were screened out from both transcriptomes, out of which 625 were upregulated and 491 were found to be downregulated. To understand the potential regulatory roles of differentially expressed genes in metabolic networks and biosynthetic pathways: KEGG mapping, gene ontology, and co-expression network analysis were performed. Comparison with Flowering Interactive Database (FLOR-ID) resulted in eight differentially expressed genes viz. CHD3-type chromatin-remodeling factor PICKLE ( PKL ), phytochrome-associated serine/threonine-protein phosphatase ( FYPP ), protein TOPLESS ( TPL ), sensitive to freezing 6 ( SFR6 ), lysine-specific histone demethylase 1 homolog 1 ( LDL1 ), pre-mRNA-processing-splicing factor 8A ( PRP8A ), sucrose synthase 4 ( SUS4 ), ubiquitin carboxyl-terminal hydrolase 12 ( UBP12 ), known to be broadly involved in flowering, photoperiodism, embryo development, and cold response pathways. Male and female flower bud transcriptome data of Sea buckthorn may provide comprehensive information at genomic level for the identification of genetic regulation involved in sex determination.

  8. [THE USE OF OZONATED SEA BUCKTHORN OIL IN THE PREVENTION AND TREATMENT OF TOBACCO DEPENDENCE PERIODONTITIS IN THE EXPERIMENT].

    Science.gov (United States)

    Zubachyk, V; Ilchyshyn, M

    2014-12-01

    Therapeutic and prophylactic properties ozonated of sea buckthorn oil in the experiment on the model of generalized periodontitis in Wistar rats induced by action of extracted products of incomplete combustion of tobacco smoke was investigated. It is proved that the proposed method of ozone therapy in combination with fitooil prevents and corrects metabolic disturbances in the periodontal tissues, caused a by high therapeutic effect of the drug.

  9. Investigation on the antidepressant effect of sea buckthorn seed oil through the GC-MS-based metabolomics approach coupled with multivariate analysis.

    Science.gov (United States)

    Tian, Jun-sheng; Liu, Cai-chun; Xiang, Huan; Zheng, Xiao-fen; Peng, Guo-jiang; Zhang, Xiang; Du, Guan-hua; Qin, Xue-mei

    2015-11-01

    Depression is one of the prevalent and serious mental disorders and the number of depressed patients has been on the rise globally during the recent decades. Sea buckthorn seed oil from traditional Chinese medicine (TCM) is edible and has been widely used for treatment of different diseases for a long time. However, there are few published reports on the antidepressant effect of sea buckthorn seed oil. With the objective of finding potential biomarkers of the therapeutic response of sea buckthorn seed oil in chronic unpredictable mild stress (CUMS) rats, urine metabolomics based on gas chromatography-mass spectrometry (GC-MS) coupled with multivariate analysis was applied. In this study, we discovered a higher level of pimelic acid as well as palmitic acid and a lower level of suberic acid, citrate, phthalic acid, cinnamic acid and Sumiki's acid in urine of rats exposed to CUMS procedures after sea buckthorn seed oil was administered. These changes of metabolites are involved in energy metabolism, fatty acid metabolism and other metabolic pathways as well as in the synthesis of neurotransmitters and it is helpful to facilitate the efficacy evaluation and mechanism elucidating the effect of sea buckthorn seed oil for depression management.

  10. African Journal of Biotechnology - Vol 11, No 45 (2012)

    African Journals Online (AJOL)

    Hippophae rhamnoides L.) against foodborne and food spoilage pathogens · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Richa Arora, Sunil Mundra, Ashish Yadav, Ravi B Srivastava, ...

  11. In vitro Evaluation of antibacterial efficacy of aqueous extracts of Iranian Native Plants on the Standard Strains of Pseudomonas aeruginosa (Short Communication

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    Saleh Jamehdor

    2014-08-01

    Results & Conclusion: In this study, the highest anti-bacterial effect have been relative to Hippophae rhamnoides (17.6 mm. The minimum inhibitory concentration of Zataria multiflora Boiss, Juglans regia L and Origanum vulgare was 25% and was recorded 12.5% for Hippophae rhamnoides. At this research, difined all of the herbal extracts have antibacterial effect on the studied strain and this activity due to the culture, origin, growing season and effective material in plants could be different.

  12. Quinine sensitivity influences the acceptance of sea-buckthorn and grapefruit juices in 9- to 11-year-old children.

    Science.gov (United States)

    Hartvig, Ditte; Hausner, Helene; Wendin, Karin; Bredie, Wender L P

    2014-03-01

    The acceptance of novel foods by children is related to a number of factors, and differences in taste sensitivity may form some specific challenges. High sensitivity might be a barrier to the acceptance of sour/bitter products by children. This study investigated the effect of sensitivity to bitter, sour, sweet, and salty tastes on the acceptance of Nordic juices in 9- to 11-year-old children. A total of 328 children were subjected to two taste sensitivity tests for quinine, citric acid, sucrose, and NaCl. Their acceptance of six juices (carrot, rosehip, sea-buckthorn, lingonberry, grapefruit, and aronia) was measured. Bitter sensitivity was found to be significantly correlated to the intake of the sweet sea-buckthorn and lingonberry juices; the most bitter-sensitive children exhibited the highest intake of these juices. The opposite relationship was found for bitter sensitivity and the intake of the bitter grapefruit juice. Sour, sweet, and salt sensitivities did not affect the intake of any of the juices. Liking scores were not affected by sensitivity. In conclusion, bitter sensitivity appears to influence food intake in children to a greater extent than sour, sweet, or salt sensitivity. Bitter-sensitive children exhibited a reduced intake of grapefruit juice and a higher intake of sucrose-sweetened juices. Thus, bitter sensitivity might be a challenge in the acceptance of certain bitter foods. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. Oral sea buckthorn oil attenuates tear film osmolarity and symptoms in individuals with dry eye.

    Science.gov (United States)

    Larmo, Petra S; Järvinen, Riikka L; Setälä, Niko L; Yang, Baoru; Viitanen, Matti H; Engblom, Janne R K; Tahvonen, Raija L; Kallio, Heikki P

    2010-08-01

    Dry eye is a common condition that can severely impair the quality of life. We aimed to find out whether oral sea buckthorn (SB) oil, containing (n-3) and (n-6) fatty acids and antioxidants, affects dry eye. In this double-blind, randomized, parallel trial, 20- to 75-y-old women and men experiencing dry eye symptoms consumed 2 g of SB or placebo oil daily for 3 mo from fall to winter. One hundred participants were recruited and 86 completed the study. Clinical dry eye tests and symptom follow-ups were performed. Tear film hyperosmolarity is a focal factor in dry eye. There was a general increase in the osmolarity from baseline to the end of the intervention. Compared with the placebo group, the increase was significantly less in the SB group when all participants were included [intention to treat (ITT), P = 0.04] and when only participants consuming the study products for at least 80% of the intervention days were included [per protocol (PP), P = 0.02]. The maximum intensities of redness and burning tended to be lower in the SB group. In the ITT participants, the group difference was significant for redness (P = 0.04) but not for burning (P = 0.05). In the PP participants, the group difference was significant for burning (P = 0.04) but not for redness (P = 0.11). In conclusion, SB oil attenuated the increase in tear film osmolarity during the cold season and positively affected the dry eye symptoms.

  14. Sea buckthorn bud extract displays activity against cell-cultured Influenza virus.

    Science.gov (United States)

    Torelli, A; Gianchecchi, E; Piccirella, S; Manenti, A; Piccini, G; Llorente Pastor, E; Canovi, B; Montomoli, E

    2015-08-05

    Vaccines and antiviral drugs are the most widely used methods of preventing or treating Influenza virus infection. The role of sea buckthorn (SBT) bud dry extract as a natural antiviral drug against Influenza was investigated. Influenza virus was cultured in the MDCK cell line, with or without SBT bud extract, and virus growth was assessed by HA and TCID50 virus titration in terms of cytopathic effect on cells. Several concentrations of extract were tested, the virus titer being measured on day 4 after infection. After infection, the virus titer in the control sample was calculated to be 2.5 TCID50/ml; treatment with SBT bud extract reduced the virus titer to 2.0 TCID50/ml at 50 μg/ml, while the HA titer was reduced from 1431 (control) to 178. Concentrations lower than 50μg/ml displayed an inhibitory effect in the HA assay, but not in the TCID50 virus titration; however, observation of the viral cultures confirmed a slowdown of viral growth at all concentrations. Natural dietary supplements and phytotherapy are a growing market and offer new opportunities for the treatment of several diseases and disorders. These preliminary experiments are the first to show that SBT bud extract is able to reduce the growth of the Influenza A H1N1 virus in vitro at a concentration of 50 μg/ml. This discovery opens up the possibility of using SBT bud extract as a valid weapon against Influenza and, in addition, as the starting-point for the discovery of new drugs. © Copyright by Pacini Editore SpA, Pisa, Italy.

  15. Changes in the contents of kaempherol, quercetin and L-ascorbic acid in sea buckthorn berries during maturation

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    N. JEPPSON

    2008-12-01

    Full Text Available The contents of kaempherol, quercetin and L-ascorbic acid in sea buckthorn berries were measured at different maturation stages using High Performance Liquid Chromatography (HPLC methods. The content of ascorbic acid decreased over time with significant differences between sampling dates for the five cultivars studied. The mean decrease was 25% in 19 days, from 1.48 to 1.10 g kg-1. Quercetin decreased whereas kaempherol increased during maturation. Among three studied cultivars, the decrease in quercetin was significant (from 0.028 to 0.014 g kg-1 in 'Otradnaja', where as the increase (from 0.012 to 0.016 g kg-1 in kaempherol was significant in the others two, 'Prozratnaja' and 'Gibrid Pertjik'.;

  16. 'Candidatus Phytoplasma spartii', 'Candidatus Phytoplasma rhamni' and 'Candidatus Phytoplasma allocasuarinae', respectively associated with spartium witches'-broom, buckthorn witches'-broom and allocasuarina yellows diseases.

    Science.gov (United States)

    Marcone, C; Gibb, K S; Streten, C; Schneider, B

    2004-07-01

    Spartium witches'-broom (SpaWB), buckthorn witches'-broom (BWB) and allocasuarina yellows (AlloY) are witches'-broom and yellows diseases of Spartium junceum (Spanish broom), Rhamnus catharticus (buckthorn) and Allocasuarina muelleriana (Slaty she-oak), respectively. These diseases are associated with distinct phytoplasmas. The SpaWB, BWB and AlloY phytoplasmas share <97.5 % 16S rDNA sequence similarity with each other and with other known phytoplasmas, including the closely related phytoplasmas of the apple proliferation group. Also, the SpaWB, BWB and AlloY phytoplasmas each have a different natural plant host. Based on their unique properties, it is proposed to designate the mentioned phytoplasmas as novel 'Candidatus' species under the names 'Candidatus Phytoplasma spartii', 'Candidatus Phytoplasma rhamni' and 'Candidatus Phytoplasma allocasuarinae', respectively.

  17. Effect of a low dose of sea buckthorn berries on circulating concentrations of cholesterol, triacylglycerols, and flavonols in healthy adults.

    Science.gov (United States)

    Larmo, Petra S; Yang, Baoru; Hurme, Saija A M; Alin, Jouni A; Kallio, Heikki P; Salminen, Eeva K; Tahvonen, Raija L

    2009-08-01

    Epidemiological studies indicate beneficial effects of flavonoids on cardiovascular disease (CVD) risk. To study the effect of flavonoid-rich sea buckthorn berry (SBB) on circulating lipid markers associated with CVD risk and plasma flavonol concentration. Also investigated was whether changes in the circulating flavonol concentrations correlate with the SBB induced changes in C-reactive protein (CRP) concentration observed previously. In all 229 healthy participants completed the randomized double-blind study and consumed daily 28 g of SBB or placebo for 3 months. Fasting blood samples for the analysis of lipid markers and flavonols were obtained at the beginning and end of the study. Compared to the placebo, the consumption of SBB increased the plasma concentration of the flavonols quercetin and isorhamnetin significantly [treatment differences 3.0 ng/ml (P = 0.03) and 3.9 ng/ml (P < 0.01), respectively]. The increase of kaempferol concentration was not significant [treatment difference 0.7 ng/ml (P = 0.08)]. SBB did not affect the serum total, HDL, LDL cholesterol, or the serum triacylglycerol concentrations. There was no correlation between the changes in flavonol and CRP concentrations of participants. The consumption of SBB significantly increased the fasting plasma concentration of quercetin and isorhamnetin indicating that it is a good dietary source of flavonols. However, this did not convert to affecting the circulating concentrations of lipid markers in healthy, normolipidemic adults having healthy diets.

  18. Effect of Sea Buckthorn Leaves on Inosine Monophosphate and Adenylosuccinatelyase Gene Expression in Broilers during Heat Stress

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    Wei Zhao

    2012-01-01

    Full Text Available The trial was conducted to evaluate the effects of sea buckthorn leaves (SBL on meat flavor in broilers during heat stress. A total 360 one-day-old Arbor Acre (AA broilers (male were randomly allotted to 4 treatments with 6 replicates pens pretreatment and 15 birds per pen. The control group was fed a basal diet, the experimental group I, II and III were fed the basal diet supplemented with 0.25%, 0.5%, 1% SBL, respectively. During the 4th week, broilers were exposed to heat stress conditions (36±2°C, after which, muscle and liver samples were collected. High performance liquid chromatography (HPLC was performed to measure the content of inosine monophosphate (IMP; Real-Time PCR was performed to determine the expression of the ADSL gene. The results showed that the content of breast muscle IMP of group I, II and III was significantly increased 68%, 102% and 103% (p<0.01 compared with the control, respectively; the content of thigh muscle IMP of group II and III was significantly increased 56% and 58% (p<0.01, respectively. Additionally, ADSL mRNA expression in group I, II and III was increased significantly 80%, 65% and 49% (p<0.01 compared with the control, respectively. The content of IMP and expression of ADSL mRNA were increased by basal diet supplemented with SBL, therefore, the decrease of meat flavor caused by heat stress was relieved.

  19. Effect of Sea Buckthorn Leaves on Inosine Monophosphate and Adenylosuccinatelyase Gene Expression in Broilers during Heat Stress.

    Science.gov (United States)

    Zhao, Wei; Chen, Xin; Yan, Changjiang; Liu, Hongnan; Zhang, Zhihong; Wang, Pengzu; Su, Jie; Li, Yao

    2012-01-01

    The trial was conducted to evaluate the effects of sea buckthorn leaves (SBL) on meat flavor in broilers during heat stress. A total 360 one-day-old Arbor Acre (AA) broilers (male) were randomly allotted to 4 treatments with 6 replicates pens pretreatment and 15 birds per pen. The control group was fed a basal diet, the experimental group I, II and III were fed the basal diet supplemented with 0.25%, 0.5%, 1% SBL, respectively. During the 4th week, broilers were exposed to heat stress conditions (36±2°C), after which, muscle and liver samples were collected. High performance liquid chromatography (HPLC) was performed to measure the content of inosine monophosphate (IMP); Real-Time PCR was performed to determine the expression of the ADSL gene. The results showed that the content of breast muscle IMP of group I, II and III was significantly increased 68%, 102% and 103% (p<0.01) compared with the control, respectively; the content of thigh muscle IMP of group II and III was significantly increased 56% and 58% (p<0.01), respectively. Additionally, ADSL mRNA expression in group I, II and III was increased significantly 80%, 65% and 49% (p<0.01) compared with the control, respectively. The content of IMP and expression of ADSL mRNA were increased by basal diet supplemented with SBL, therefore, the decrease of meat flavor caused by heat stress was relieved.

  20. Comparative Fingerprint of Aromatic Herbs and Yeast Alcoholic Extracts used as Ingredients for Promen, a Prostate Preventive Nutraceutical

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    Florina Csernatoni

    2013-11-01

    Full Text Available The aim of this study was to characterize and identify different bioactive compounds in plant sources and yeast powders  to obtain an original nutraceutical (Promen which has beneficial effects in prostate disease prevention. Seven plant and fruit sources, namely nettle (Urtica dioica, green tea (Camellia sinensis, fluff with small flowers (Epilobium parviplorum, tomato (Solanum licopersicum,  sea buckthorn (Hippophae rhamnoides, pumpkin (Cucurbita maxima, sunflower (Helianthus annus and lyophilized beer yeast (Saccharomyces cerevisiae were investigated. Methanolic extracts were prepared using 15% plant concentration and the purified fractions were analyzed using high throughput techniques like UV-VIS spectroscopy, high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD and mass spectrometry LC-QTOF -MS. The majority of the investigated plants were rich in phenolic derivatives, polyphenols (flavonoid glucosides, while yeast was rich in aminoacids, peptides and vitamins B. The major compounds identified were: Juglone, Resveratrol, Quercetin, Epigallocatechin, Gallocatechin, Biochanin A, Isorhamnetin 3-O-glucoside 7-O-rhamnoside, Quercetin 3-O-galactoside 7-O-rhamnoside, Kaempferol 3,7-O-diglucoside and p-Coumaroylquinic acid. The specific biomarkers were identified for both plant extracts used as ingredients to obtain an nutraceutical  Promen. Combined UV-Vis spectroscopy, HPLC-PDA chromatography and LC-MS spectrometry are recommended as accurate, sensible and reliable tools to investigate the plants and nutraceutical fingerprints and to predict the relation between ingredients composition and their health effects.

  1. Differential transcriptome analysis reveals genes related to cold tolerance in seabuckthorn carpenter moth, Eogystia hippophaecolus.

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    Mingming Cui

    Full Text Available Seabuckthorn carpenter moth, Eogystia hippophaecolus (Lepidoptera: Cossidae, is an important pest of sea buckthorn (Hippophae rhamnoides, which is a shrub that has significant ecological and economic value in China. E. hippophaecolus is highly cold tolerant, but limited studies have been conducted to elucidate the molecular mechanisms underlying its cold resistance. Here we sequenced the E. hippophaecolus transcriptome using RNA-Seq technology and performed de novo assembly from the short paired-end reads. We investigated the larval response to cold stress by comparing gene expression profiles between treatments. We obtained 118,034 unigenes, of which 22,161 were annotated with gene descriptions, conserved domains, gene ontology terms, and metabolic pathways. These resulted in 57 GO terms and 193 Kyoto Encyclopedia of Genes and Genomes (KEGG pathways. By comparing transcriptome profiles for differential gene expression, we identified many differentially expressed proteins and genes, including heat shock proteins and cuticular proteins which have previously been reported to be involved in cold resistance of insects. This study provides a global transcriptome analysis and an assessment of differential gene expression in E. hippophaecolus under cold stress. We found seven differential expressed genes in common between developmental stages, which were verified with qPCR. Our findings facilitate future genomic studies aimed at improving our understanding of the molecular mechanisms underlying the response of insects to low temperatures.

  2. The Influence of Some Phytobiotics on Haematological and Some Biochemical Indices at Oreochromis Niloticus – Linnaeus, 1758

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    Alina Antache

    2014-05-01

    Full Text Available The aim of this research was to evaluated the influence of some phytobiotics on haematological profile, leukocyte reaction and some biochemical indices at Oreochromis niloticus species, reared in a recirculating aquaculture system. This experiment was conducted six weeks. The experimental variants were: V1 – control; V2 – 1% Rosmarinus officinalis / kg feed; V3 – 1% Hippophae rhamnoides / kg feed and V4 – 1% Zingiber officinale / kg feed. Blood was analyzed using standard techniques. At the end of the experiment the following parameters were determined: RBCc (x106cells/µL, Hb (g/dL, PVC (%, MCV (µm3, MCH (pg, MCHC (g/dL, TP (g/dL, GLU (mg/dL, cortisol (ng/mL, lysozyme activity (U/mL, absolute number of blood cells (x103 cells/µL and leukogram (%. The results showed that the administration in feed of some phytobiotics lead to signifiant differences (p<0.05 of following parameters: RBCc (x106cells/µL, MCV (µm3, glucose (mg/dL, lysozyme activity (U/mL, monocyte (% and in absolute number of leukocytes, lymphocytes and monocytes. In conclusion, due to decreasing of RBCc, PVC, Hb, MCHC, cortisol, GLU and due to normal concentration of TP, we can say that the administration of sea buckthorn and ginger, but even rosemary administration, in diet improves the physiological status at Oreochromis niloticus species.

  3. Effects of sea buckthorn and bilberry on serum metabolites differ according to baseline metabolic profiles in overweight women: a randomized crossover trial1234

    Science.gov (United States)

    Larmo, Petra S; Kangas, Antti J; Soininen, Pasi; Lehtonen, Henna-Maria; Suomela, Jukka-Pekka; Yang, Baoru; Viikari, Jorma; Ala-Korpela, Mika; Kallio, Heikki P

    2013-01-01

    Background: Berries are associated with health benefits. Little is known about the effect of baseline metabolome on the overall metabolic responses to berry intake. Objective: We studied the effects of berries on serum metabolome. Design: Eighty overweight women completed this randomized crossover study. During the interventions of 30 d, subjects consumed dried sea buckthorn berries (SBs), sea buckthorn oil (SBo), sea buckthorn phenolics ethanol extract mixed with maltodextrin (SBe+MD) (1:1), or frozen bilberries. Metabolic profiles were quantified from serum samples by using 1H nuclear magnetic resonance spectroscopy. Results: All interventions induced a significant (P < 0.001–0.003) effect on the overall metabolic profiles. The effect was observed both in participants who had a metabolic profile that reflected higher cardiometabolic risk at baseline (group B: P = 0.001–0.008) and in participants who had a lower-risk profile (group A: P < 0.001–0.009). Although most of the changes in individual metabolites were not statistically significant after correction for multiplicity, clear trends were observed. SB-induced effects were mainly on serum triglycerides and very-low-density lipoprotein (VLDL) and its subclasses, which decreased in metabolic group B. SBo induced a decreasing trend in serum total, intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL) cholesterol and subfractions of IDL and LDL in group B. During the SBe+MD treatment, VLDL fractions and serum triglycerides increased. Bilberries caused beneficial changes in serum lipids and lipoproteins in group B, whereas the opposite was true in group A. Conclusion: Berry intake has overall metabolic effects, which depend on the cardiometabolic risk profile at baseline. This trial was registered at clinicaltrials.gov as NCT01860547. PMID:23945716

  4. Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways.

    Science.gov (United States)

    Jiang, Fan; Guan, Haining; Liu, Danyi; Wu, Xi; Fan, Mingcheng; Han, Jianchun

    2017-03-22

    Sea buckthorn has long been used as a functional food to regulate cholesterol, relieve angina, and diminish inflammation. Flavonoids are one of the main active components in sea buckthorn. We investigated the effects of sea buckthorn flavonoid (SF) treatment on two pathways that mediate inflammation, the mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) pathways, to explore the anti-inflammatory activity of SFs in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The LPS-induced over-production of nitric oxide (NO) and prostaglandin E2 (PGE 2 ) was inhibited by SFs through a mechanism related to the modulatory effects of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) genes. Additionally, SFs downregulated the production and mRNA expression of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β. Moreover, SFs inhibited the phosphorylation of the p38 and stress-activated protein kinase/jun amino-terminal kinase (SAPK/JNK) MAPK pathways, and they reduced the nuclear translocation of NF-κB to prevent its activation by blocking the phosphorylation and degradation of inhibitor protein of NF-κB α (IκB-α). Based on these findings, SFs may exert their inhibitory effects on inflammation by regulating the release of inflammatory mediators through the MAPK and NF-κB pathways. SFs highlight the potential benefits of using functional foods with anti-inflammatory actions to combat inflammatory diseases.

  5. Effects of sea buckthorn and bilberry on serum metabolites differ according to baseline metabolic profiles in overweight women: a randomized crossover trial.

    Science.gov (United States)

    Larmo, Petra S; Kangas, Antti J; Soininen, Pasi; Lehtonen, Henna-Maria; Suomela, Jukka-Pekka; Yang, Baoru; Viikari, Jorma; Ala-Korpela, Mika; Kallio, Heikki P

    2013-10-01

    Berries are associated with health benefits. Little is known about the effect of baseline metabolome on the overall metabolic responses to berry intake. We studied the effects of berries on serum metabolome. Eighty overweight women completed this randomized crossover study. During the interventions of 30 d, subjects consumed dried sea buckthorn berries (SBs), sea buckthorn oil (SBo), sea buckthorn phenolics ethanol extract mixed with maltodextrin (SBe+MD) (1:1), or frozen bilberries. Metabolic profiles were quantified from serum samples by using (1)H nuclear magnetic resonance spectroscopy. All interventions induced a significant (P < 0.001-0.003) effect on the overall metabolic profiles. The effect was observed both in participants who had a metabolic profile that reflected higher cardiometabolic risk at baseline (group B: P = 0.001-0.008) and in participants who had a lower-risk profile (group A: P < 0.001-0.009). Although most of the changes in individual metabolites were not statistically significant after correction for multiplicity, clear trends were observed. SB-induced effects were mainly on serum triglycerides and very-low-density lipoprotein (VLDL) and its subclasses, which decreased in metabolic group B. SBo induced a decreasing trend in serum total, intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL) cholesterol and subfractions of IDL and LDL in group B. During the SBe+MD treatment, VLDL fractions and serum triglycerides increased. Bilberries caused beneficial changes in serum lipids and lipoproteins in group B, whereas the opposite was true in group A. Berry intake has overall metabolic effects, which depend on the cardiometabolic risk profile at baseline. This trial was registered at clinicaltrials.gov as NCT01860547.

  6. Effect of storage time and temperature on stability of bioactive compounds in aseptically packed beverages prepared from rose hips and sea buckthorn berries

    Directory of Open Access Journals (Sweden)

    Staffan C Andersson

    2015-12-01

    Full Text Available Rose hips and sea buckthorn berries contain high amounts of beneficial bioactive compounds. In this study the content and stability of natural vitamins and antioxidants were investigated in two fruit beverages made from rose hip powder, sea buckthorn berry purée and white grape juice concentrate, and wheat germ oil in one of the beverages. Beverages were formulated, pasteurised and filled aseptically in Tetra Brik packages and stored up to 35 days at 4 °C or 22 °C. Samples were analysed for the content of ascorbic acid, carotenoids, major phenolics, tocochromanols, total phenols and total antioxidant capacity as ferric reducing ability. The largest changes occurred during the first 5 days of storage, thereafter mainly ascorbic acid decreased. In the wheat germ oil supplemented beverage, α-tocopherol was more rapidly degraded. Negative correlations between the decrease of some carotenoids and tocochromanols, and the increase of some phenolics, suggest relationships of redox reactions specific to the amount of oil supplemented. From the present study we can conclude that additions of oil to beverages should be carefully selected to avoid oxidations of bioactive compounds. Furthermore, packaging of the beverages should be carried out at higher temperatures and thereafter it should be stored at low temperatures (4 °C, which decreased oxidation of all bioactive compounds except ascorbic acid.

  7. Effects of flavones of sea buckthorn fruits on growth performance, carcass quality, fat deposition and lipometabolism for broilers.

    Science.gov (United States)

    Ma, J S; Chang, W H; Liu, G H; Zhang, S; Zheng, A J; Li, Y; Xie, Q; Liu, Z Y; Cai, H Y

    2015-11-01

    The objective of this study was to evaluate the effects of different levels of flavones of sea buckthorn fruits (FSBF) on growth performance, carcass quality, fat deposition, and lipometabolism for broilers. 240 one-day-old Arbor Acres male broilers were randomly allotted to 4 dietary treatments (0, 0.05%, 0.10%, and 0.15% FSBF) with 6 replicates of 10 birds. Broilers were reared for 42 d. Results showed FSBF quadratically improved average daily feed intake (ADFI), average daily gain (ADG), and final body weight (BW) (P = 0.002, P = 0.019 and P = 0.018, respectively). The abdominal fat percentage in 0.05%, 0.10%, and 0.15% FSBF supplementation groups was decreased by 21.08%, 19.12%, and 19.61% with respect to the control group, respectively (P < 0.05). The intramuscular fat (IMF) content in the breast muscle of the broilers was increased by 7.21%, 23.42% and 6.30% in 0.05%, 0.10% and 0.15% FSBF groups, and that in the thigh meat was raised by 4.43%, 24.63% and 12.32%, compared with the control group, respectively (P < 0.05). FSBF had a quadratic effect on the abdominal fat percentage and IMF in the breast muscle (P < 0.05). Dietary FSBF also modified fatty acids of muscular tissues, resulting in a higher ratio of unsaturated to saturated fatty acids (P < 0.05). Supplementing FSBF in the diet greatly decreased the levels of triglyceride, cholesterol, and low-density lipoprotein cholesterol (P < 0.05). Moreover, the quadratic responses were also observed in the levels of insulin and adiponectin in serum (P = 0.020 and P = 0.037, respectively). Abdominal fat percentage was correlated negatively with insulin and positively with adiponectin (P < 0.05). IMF content in the breast and thigh muscles were correlated positively with insulin, and negatively with adiponectin (P < 0.05). A positive correlation existed between breast muscle, IMF, and leptin (P < 0.05). In conclusion, adding FSBF into the diets affected growth performance and fat deposition of broilers by

  8. Protective and ameliorative effect of sea buckthorn leaf extract supplementation on lead induced hemato-biochemical alterations in Wistar rats.

    Science.gov (United States)

    Zargar, Rizwana; Raghuwanshi, Pratiksha; Rastogi, Ankur; Koul, Aditi Lal; Khajuria, Pallavi; Ganai, Aafreen Wahid; Kour, Sumeet

    2016-09-01

    To evaluate the protective and ameliorative effect of aqueous sea buckthorn leaf extract (SLE) on hemato-biochemical profile in lead intoxicated Wistar rats. An experiment was conducted for 60 days. 36 adult male Wistar rats with a mean body weight of 177.8±12.6 g were divided into five groups and were subjected to various daily oral treatment regimens. Group I served as a negative control receiving only feed and water, Group II (positive control for lead) received lead acetate at 250 ppm in drinking water, and Group III (positive control for SLE) received SLE at 100 mg/kg b.wt. Animals in Group IV received a combination of lead acetate at 250 ppm in drinking water for the first 45 days and SLE at 100 mg/kg b.wt. throughout the experimental period of 60-day, and in Group V for the last 15 days of the trial after the administration of lead acetate until the first 45 days of the trial to study the protective and ameliorating effects of SLE, respectively. Blood samples were collected from retro-orbital fossa of each rat on 0 th , 45 th , and 60 th day of the experiment for hemato-biochemical analysis including hemoglobin (Hb), packed cell volume (PCV), serum total protein, albumin, globulin, albumin:globulin ratio, cholesterol, urea, and creatinine. Significantly (p<0.01) lower levels of serum total proteins and albumin, and a significantly (p<0.01) higher serum cholesterol, urea and creatinine levels were observed in Group II (lead intoxicated group) in comparison to Group I (negative control). Administration of SLE at 100 mg/kg body wt. to lead intoxicated Wistar rats resulted in normalization of almost all the biochemical parameters studied in both the treatment Groups, i.e., IV and V (protective and ameliorative). However, the effects were more pronounced in the protective group. No effects of SLE supplementation were observed on Hb levels. PCV levels improved in protective groups, but no effect was observed in ameliorative group in comparison to lead intoxicated

  9. Protective and ameliorative effect of sea buckthorn leaf extract supplementation on lead induced hemato-biochemical alterations in Wistar rats

    Directory of Open Access Journals (Sweden)

    Rizwana Zargar

    2016-09-01

    Full Text Available Aim: To evaluate the protective and ameliorative effect of aqueous sea buckthorn leaf extract (SLE on hemato-biochemical profile in lead intoxicated Wistar rats. Materials and Methods: An experiment was conducted for 60 days. 36 adult male Wistar rats with a mean body weight of 177.8±12.6 g were divided into five groups and were subjected to various daily oral treatment regimens. Group I served as a negative control receiving only feed and water, Group II (positive control for lead received lead acetate at 250 ppm in drinking water, and Group III (positive control for SLE received SLE at 100 mg/kg b.wt. Animals in Group IV received a combination of lead acetate at 250 ppm in drinking water for the first 45 days and SLE at 100 mg/kg b.wt. throughout the experimental period of 60-day, and in Group V for the last 15 days of the trial after the administration of lead acetate until the first 45 days of the trial to study the protective and ameliorating effects of SLE, respectively. Blood samples were collected from retro-orbital fossa of each rat on 0th, 45th, and 60th day of the experiment for hemato-biochemical analysis including hemoglobin (Hb, packed cell volume (PCV, serum total protein, albumin, globulin, albumin:globulin ratio, cholesterol, urea, and creatinine. Results: Significantly (p<0.01 lower levels of serum total proteins and albumin, and a significantly (p<0.01 higher serum cholesterol, urea and creatinine levels were observed in Group II (lead intoxicated group in comparison to Group I (negative control. Administration of SLE at 100 mg/kg body wt. to lead intoxicated Wistar rats resulted in normalization of almost all the biochemical parameters studied in both the treatment Groups, i.e., IV and V (protective and ameliorative. However, the effects were more pronounced in the protective group. No effects of SLE supplementation were observed on Hb levels. PCV levels improved in protective groups, but no effect was observed in

  10. Sea Buckthorn Fruit Oil Extract Alleviates Insulin Resistance through the PI3K/Akt Signaling Pathway in Type 2 Diabetes Mellitus Cells and Rats.

    Science.gov (United States)

    Gao, Shan; Guo, Qing; Qin, Chengguang; Shang, Rui; Zhang, Zesheng

    2017-02-22

    Sea buckthorn fruit oil is rich in palmitoleic acid (POA), which has been reported to play roles in many metabolic processes. In this study, a sea buckthorn fruit oil (SBFO) extract was evaluated through in vitro experiments (the doses were 50, 100, 200, and 400 μM) and in vivo experiments (the doses were 100, 200, and 300 mg/kg·day) to explore its mechanism of action in the treatment of type 2 diabetes mellitus (T2DM). The results revealed that the SBFO extract effectively increased the glucose uptake from 12.23 ± 1.09 to 14.90 ± 1.48 mmol/L in insulin resistance (IR) HepG2 cells, lowered blood glucose (the reductions rates of blood glucose in groups treated with SBFO extract at 200 and 300 mg/kg·day were 10.47% and 13.79%, respectively) and improved insulin indices from -6.11 ± 0.10 to -5.45 ± 0.31 after 4 weeks treatment with SBFO extract at 300 mg/kg·day in T2DM SD rats. RT-PCR and Western blotting analyses suggested that the SBFO extract could promote the expression of phosphatidylinositol-3-kinase (PI3K) and glycogen synthesis (GS) while inhibiting the expression of glycogen synthesis kinase-3β (GSK-3β). Thus, the SBFO extract played a positive role in alleviating T2DM through the PI3K/Akt signaling pathway in HepG2 cells, and diabetic rats and could be used for the future development of functional food and dietary supplements.

  11. Sea Buckthorn Pomace Supplementation in the Diet of Growing Pigs—Effects on Fatty Acid Metabolism, HPA Activity and Immune Status

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    Dirk Dannenberger

    2018-02-01

    Full Text Available There is evidence that sea buckthorn, as a source of n-3 polyunsaturated fatty acids (n-3 PUFA, possesses health-enhancing properties and may modulate neuroendocrine and immune functions. In the present study, we investigated the effect of sea buckthorn pomace (SBP supplementation in the diet of growing German Landrace pigs on fatty acids in the blood and hypothalamus, peripheral immune parameters and mRNA expression of corticotropin-releasing hormone (CRH, mineralocorticoid receptor (MR and glucocorticoid receptor (GR in the hypothalamus and spleen. Pigs were fed diets supplemented with 12% of dried SBP or 0% SBP (control group over an intervention period of eight weeks. The fatty acid profiles in blood plasma were significantly affected by SBP supplementation only for C18:2n-6 and n-6/n-3 PUFA ratio compared with the control group. SBP supplementation did not significantly affect the fatty acid concentrations in the hypothalamus. Furthermore, there were no significant differences in mRNA expression of CRH, MR and GR in the hypothalamus or of GR mRNA expression in the spleen. Concerning the immune status, the plasma IgG levels tended to be higher in SBP pigs, whereas the leukocyte distribution, mitogen-stimulated lymphocyte proliferation, and serum IgM levels remained unchanged. In conclusion, the SBP supplementation of the diet only caused moderate effects on fatty acid metabolism, but no significant effects on hypothalamic–pituitary–adrenal (HPA activity and immunity in growing pigs. It seems that a beneficial effect of dietary n-3 PUFA on health and welfare is more likely to be expected during stressful situations.

  12. Sea Buckthorn Pomace Supplementation in the Diet of Growing Pigs-Effects on Fatty Acid Metabolism, HPA Activity and Immune Status.

    Science.gov (United States)

    Dannenberger, Dirk; Tuchscherer, Margret; Nürnberg, Gerd; Schmicke, Marion; Kanitz, Ellen

    2018-02-21

    There is evidence that sea buckthorn, as a source of n -3 polyunsaturated fatty acids ( n -3 PUFA), possesses health-enhancing properties and may modulate neuroendocrine and immune functions. In the present study, we investigated the effect of sea buckthorn pomace (SBP) supplementation in the diet of growing German Landrace pigs on fatty acids in the blood and hypothalamus, peripheral immune parameters and mRNA expression of corticotropin-releasing hormone (CRH), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the hypothalamus and spleen. Pigs were fed diets supplemented with 12% of dried SBP or 0% SBP (control group) over an intervention period of eight weeks. The fatty acid profiles in blood plasma were significantly affected by SBP supplementation only for C18:2 n -6 and n -6/ n -3 PUFA ratio compared with the control group. SBP supplementation did not significantly affect the fatty acid concentrations in the hypothalamus. Furthermore, there were no significant differences in mRNA expression of CRH, MR and GR in the hypothalamus or of GR mRNA expression in the spleen. Concerning the immune status, the plasma IgG levels tended to be higher in SBP pigs, whereas the leukocyte distribution, mitogen-stimulated lymphocyte proliferation, and serum IgM levels remained unchanged. In conclusion, the SBP supplementation of the diet only caused moderate effects on fatty acid metabolism, but no significant effects on hypothalamic-pituitary-adrenal (HPA) activity and immunity in growing pigs. It seems that a beneficial effect of dietary n -3 PUFA on health and welfare is more likely to be expected during stressful situations.

  13. In silico–based combinatorial pharmacophore modelling and ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Biosciences; Volume 38; Issue 4. In silico -- based ... Actinorhizal plants; ADMET; Discovery Studio 3.5; Hippophae; QSAR ... Docking with Discovery Studio 3.5 revealed that two compounds from H. salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, ...

  14. Enhanced adsorptive removal of Safranine T from aqueous solutions by waste sea buckthorn branch powder modified with dopamine: Kinetics, equilibrium, and thermodynamics

    Science.gov (United States)

    Xu, Xiaohui; Bai, Bo; Wang, Honglun; Suo, Yourui

    2015-12-01

    Polydopamine coated sea buckthorn branch powder (PDA@SBP) was facilely synthesized via a one-pot bio-inspired dip-coating approach. The as-synthesized PDA@SBP was characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The adsorption progresses of Safranine T on the surface of PDA@SBP adsorbent were systematically investigated. More specifically, the effects of solution pH, contact time, initial concentration and temperature were evaluated, respectively. The experimental results showed the adsorption capacity of PDA@SBP at 293.15 K could reach up to 54.0 mg/g; the adsorption increased by 201.7% compared to that of native SBP (17.9 mg/g). Besides, kinetics studies showed that pseudo-second-order kinetic model adequately described the adsorption behavior. The adsorption experimental data could be fitted well a Freundlich isotherm model. Thermodynamic analyses showed that the ST adsorption was a physisorption endothermic process. Regeneration of the spent PDA@SBP adsorbent was conducted with 0.1 M HCl without significant reduction in adsorption capacity. On the basis of these investigations, it is believed that the PDA@SBP adsorbent could have potential applications in sewage disposal areas because of their considerable adsorption capacities, brilliant regeneration capability, and cost-effective and eco-friendly preparation and use.

  15. Response surface optimization of extraction protocols to obtain phenolic rich antioxidant from sea buckthorn and their potential application into model meat system.

    Science.gov (United States)

    Wagh, Rajesh V; Chatli, Manish K

    2017-05-01

    In the present study, processing parameters for the extraction of phenolic rich sea buckthorn seed (SBTE) extract were optimised using response surface method and subjected for in vitro efficacy viz. total phenolic, ABTS, DPPH and SASA activity. The optimised model depicted MeOH as a solvent at 60% concentration level with a reaction time of 20 min and extracting temperature of 55 °C for the highest yield and total phenolic content. The efficacy of different concentration of obtained SBT was evaluated in raw ground pork as a model meat system on the basis of various physico-chemical, microbiological, sensory quality characteristics. Addition of 0.3% SBTE significantly reduced the lipid peroxidation (PV, TBARS and FFA) and improved instrumental colour ( L* , a*, b* ) attributes of raw ground pork during refrigerated storage of 9 days. Results concluded that SBTE at 0.3% level can successfully improve the oxidative stability, microbial, sensory quality attributes in the meat model system.

  16. Coefficients of apparent absorption of the dietary nutrients from broiler feeds that include oil industry by-products (rapeseeds, grapes, buckthorn, flax and pumpkin meals

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    Tatiana Dumitra PANAITE

    2017-05-01

    Full Text Available A 42-day feeding trial was conducted on 120, day-old ROSS 308 chicks assigned to 4 (C; E1; E2; E3 groups. The broilers were housed in three-tier digestibility cages (5 broilers/cage which allowed the daily recording of the feed intake and excreta located in an experimental hall with controlled environmental conditions (27.07±2.750 C; humidity 64,80±9,57%; light regimen 23h. During the starter stage (1-10 days all chicks received a conventional compound feed (C. During the grower and finisher stages, compared to C formulation, the experimental diets different proportions, varying with the stage of development, of rapeseeds and grape meals (E1, buckthorn and flax meals (E2, pumpkin meal (lot E3. The balance study was conducted for 5 days, during weeks 4 and 6. During this period, average weights from each two cages (6 samples/group of feed consumed and excreta voided (dry matter basis were recorded and sampled for: dry matter, nitrogen, fat, fibre, ash and gross energy analysis. The chicks from E1 group had a lower (P≤0.05 coefficient of dietary nitrogen absorption than E2 chicks. The coefficients of apparent absorption of the dietary fat varied with the period of development. During the finishing stage, E3 chicks had a higher (P≤0.05 coefficient of fibre absorption than groups C, E1 and E2.

  17. Restoration of Tear Secretion in a Murine Dry Eye Model by Oral Administration of Palmitoleic Acid

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    Shigeru Nakamura

    2017-04-01

    Full Text Available Sea buckthorn (Hippophae rhamnoides–derived products have traditionally been used as food and medicinal ingredients in Eastern countries. The purpose of this study was to investigate the effect of oral intake of sea buckthorn oil products on tear secretion using a murine dry eye model. Orally administered sea buckthorn pulp oil (not seed oil restored aqueous tear secretion to its normal value under a dry eye condition. Palmitoleate (C16:1, a fatty acid present in sea buckthorn pulp oil, preserved tear secretion and suppressed inflammatory cytokines in the lacrimal gland to the same extent as that by pulp oil. These results suggest that an oral intake of sea buckthorn pulp oil has a potency to preserve tear secretion capacity in the dry eye state and palmitoleate, its main constituent fatty acid, is an active component of the oil. This effect may enable a potent diet-based treatment for the prevention of dry eye.

  18. Berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease.

    Science.gov (United States)

    Seeram, Navindra P

    2008-02-13

    An overwhelming body of research has now firmly established that the dietary intake of berry fruits has a positive and profound impact on human health, performance, and disease. Berry fruits, which are commercially cultivated and commonly consumed in fresh and processed forms in North America, include blackberry ( Rubus spp.), black raspberry ( Rubus occidentalis), blueberry ( Vaccinium corymbosum), cranberry (i.e., the American cranberry, Vaccinium macrocarpon, distinct from the European cranberry, V. oxycoccus), red raspberry ( Rubus idaeus) and strawberry ( Fragaria x ananassa). Other berry fruits, which are lesser known but consumed in the traditional diets of North American tribal communities, include chokecherry ( Prunus virginiana), highbush cranberry ( Viburnum trilobum), serviceberry ( Amelanchier alnifolia), and silver buffaloberry ( Shepherdia argentea). In addition, berry fruits such as arctic bramble ( Rubus articus), bilberries ( Vaccinuim myrtillus; also known as bog whortleberries), black currant ( Ribes nigrum), boysenberries ( Rubus spp.), cloudberries ( Rubus chamaemorus), crowberries ( Empetrum nigrum, E. hermaphroditum), elderberries ( Sambucus spp.), gooseberry ( Ribes uva-crispa), lingonberries ( Vaccinium vitis-idaea), loganberry ( Rubus loganobaccus), marionberries ( Rubus spp.), Rowan berries ( Sorbus spp.), and sea buckthorn ( Hippophae rhamnoides), are also popularly consumed in other parts of the world. Recently, there has also been a surge in the consumption of exotic "berry-type" fruits such as the pomegranate ( Punica granatum), goji berries ( Lycium barbarum; also known as wolfberry), mangosteen ( Garcinia mangostana), the Brazilian açaí berry ( Euterpe oleraceae), and the Chilean maqui berry ( Aristotelia chilensis). Given the wide consumption of berry fruits and their potential impact on human health and disease, conferences and symposia that target the latest scientific research (and, of equal importance, the dissemination of

  19. The multifunctionality of berries toward blood platelets and the role of berry phenolics in cardiovascular disorders.

    Science.gov (United States)

    Olas, Beata

    2017-09-01

    Diet and nutrition have an important influence on the prophylaxis and progression of cardiovascular disease; one example is the inhibition of blood platelet functions by specific components of fruits and vegetables. Garlic, onion, ginger, dark chocolate and polyunsaturated fatty acids all reduce blood platelet aggregation. A number of fruits contain a range of cardioprotective antioxidants and vitamins, together with a large number of non-nutrient phytochemicals such as phenolic compounds, which may possess both antioxidant properties and anti-platelet activity. Fresh berries and berry extracts possess high concentrations of phenolic compounds, i.e. phenolic acid, stilbenoids, flavonoids and lignans. The aim of this review article is to provide an overview of current knowledge of the anti-platelet activity of berries, which form an integral part of the human diet. It describes the effects of phenolic compounds present in a number of berries, i.e. black chokeberries - aronia berries (Aronia melanocarpa), blueberries (Vaccinium myrtillus), cranberries (Vaccinium sect. Oxycoccus), sea buckthorn berries (Hippophae rhamnoides) and grapes (Vitis), as well as various commercial products from berries (i.e. juices), on platelets and underlying mechanisms. Studies show that the effects of berries on platelet activity are dependent on not only the concentrations of the phenolic compounds in the berries or the class of phenolic compounds, but also the types of berry and the form (fresh berry, juice or medicinal product). Different results indicate that berries may play a role in the prevention of cardiovascular disorders, but the development of well-controlled clinical studies with berries is encouraged.

  20. Testing some etracts of plants from the Danube Delta with potential antiparasitic effect on equines

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    RĂILEANU Stefan

    2016-12-01

    Full Text Available The study was done during november 2016 on strongyls eggs from feces from a total of 28 horses in Tulcea county, C.A. Rosetti locality in the Danube Delta. To determine the degree of infestation was used the method McMaster and Stoll (adapted for quantitative larvohelmintology. The tests of hatching eggs (egg hatching assay - EHA and larval development (larvae development assay - LDA were made, using as active substances herbal decoctions and hydro-alcoholic solutions made from well known anthelmintic plants. One decoction and two hydro-alcoholic extracts were tested, Hippophae rhamnoides (sea buckthorn respectively Thymus serpyllum (creeping thyme and Arthemisia absinthium (wormwood. In order to achieve the two tests for therapeutic efficacy of the tested products were obtained six serial dilutions with concentrations of 50%, 25%, 12.5%, 6.25%, 3.12%, and 1, 56%. For decoctions were made control samples with distilled water, and with alcohol for hydroalcoholic solutions. The data obtained in both tests were analyzed using Anthelmintic Rezistance Program (ARP, determining the parameters "a" and "b" to trace the line prediction and lethal concentrations (CL. We can be concluded that for the decoction was observed an emphatic decrease of the percentage of larval development. For the two hydro-alcoholic extracts the hatching percentage of Strongyls eggs was different depending on the concentration used, the maximum value being registered at 25% dilution, in which the percentage of hatching was 82.35%, and the minimum concentration of 12.50%. LDA test showed a significant reduction in the percentage of larval Strongyls development, stage 3, at all dilutions, which is less than 50%. Key

  1. Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert.

    Science.gov (United States)

    Dhar, Priyanka; Bajpai, Prabodh Kumar; Tayade, Amol Bapurao; Chaurasia, Om Prakash; Srivastava, Ravi Bihari; Singh, Shashi Bala

    2013-10-07

    Himalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca) and roseroot (Rhodiola imbricata) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and nitric oxide (NO) radical scavenging capacities and lipid peroxidation inhibition (LPI) property of the extracts were determined. Total antioxidant power was determined by ferric reducing/antioxidant power (FRAP) assay. Total polyphenol, flavonoid, flavonol, proanthocyanidin and carotenoid were also estimated for both extracts. We have identified and quantified the phyto-chemotypes present in the methanol and n-hexane extracts by hyphenated gas chromatography/mass spectrometry (GC/MS) technique. Antioxidant capacity assays using DPPH, ABTS, NO, LPI and FRAP exhibited analogous results where the phytococktail showed high antioxidant action. The phytococktail was also found to possess high quantity of total polyphenol, flavonoid, flavonol and carotenoid. A significant and linear correlation was found between the antioxidant capacities and bioactive principles. A total of 32 phyto-chemotypes were identified from these extracts by GC/MS chemometric fingerprinting. Major phyto-chemotypes identified by GC/MS were glycosides, phenylpropanoids and derivatives, terpenoids, alkaloids, phytosterols, fatty acids and esters, alkaloids and derivatives, organic acid esters and aromatic ethers with positive biological and pharmacological actions. The phytococktail extracts were found to contain considerable amount of diverse bioactive compounds with high antioxidant capacities. The presence of hydrophilic and lipophilic antioxidants in the phytococktail could have

  2. Effects of sea buckthorn oil intake on vaginal atrophy in postmenopausal women: a randomized, double-blind, placebo-controlled study.

    Science.gov (United States)

    Larmo, Petra S; Yang, Baoru; Hyssälä, Juha; Kallio, Heikki P; Erkkola, Risto

    2014-11-01

    Vaginal atrophy, the thinning and drying of vaginal mucosa, is associated with menopause. The standard estrogen treatment is not suitable for all women. To investigate the effects of oral sea buckthorn (SB) oil supplementation on vaginal atrophy. A total of 116 postmenopausal women experiencing symptoms of vaginal dryness, itching or burning were randomized to this placebo-controlled, double-blind study. Ninety-eight participants completed the intervention of three months, during which they consumed 3g of SB or placebo oil daily. At the beginning and end, factors of vaginal health were scored by a gynecologist, vaginal pH and moisture were measured and vaginal health index was calculated. Symptoms of atrophy and menopause were evaluated at study visits and by daily logbooks. Serum samples were collected for the analysis of circulating lipids, liver enzymes and C-reactive protein. Compared to placebo, there was a significantly better rate of improvement in the integrity of vaginal epithelium in the SB group when both compliant and noncompliant participants were included (odds ratio (OR)=3.1, 95% CI 1.11-8.95). A beneficial trend was observed when only the compliant participants were included (OR=2.9; 95% CI 0.99-8.35). There was a tendency (P=0.08) toward better improvement of vaginal health index from baseline to the end in the SB group [(0.8 (SD 2.8)] compared to placebo [-0.1 (SD 2.0)]. SB oil showed beneficial effects on vaginal health, indicating it is a potential alternative for mucosal integrity for those women not able to use estrogen treatment for vaginal atrophy. Copyright © 2014 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  3. Sea Buckthorn Leaf Extract Protects Jejunum and Bone Marrow of 60Cobalt-Gamma-Irradiated Mice by Regulating Apoptosis and Tissue Regeneration

    Science.gov (United States)

    Gupta, Manish; Saini, Manu; Abdin, M. Z.; Prasad, Jagdish

    2015-01-01

    A single dose (30 mg/kg body weight) of standardized sea buckthorn leaf extract (SBL-1), administered 30 min before whole body 60Co-gamma-irradiation (lethal dose, 10 Gy), protected >90% of mice population. The purpose of this study was to investigate the mechanism of action of SBL-1 on jejunum and bone marrow, quantify key bioactive compounds, and analyze chemical composition of SBL-1. Study with 9-week-old inbred male Swiss albino Strain ‘A' mice demonstrated that SBL-1 treatment before 60Co-gamma-irradiation (10 Gy) significantly (p < 0.05) countered radiation induced decreases in jejunum crypts (1.27-fold), villi number (1.41-fold), villus height (1.25-fold), villus cellularity (2.27-fold), cryptal Paneth cells (1.89-fold), and Bcl2 level (1.54-fold). It countered radiation induced increases in cryptal apoptotic cells (1.64-fold) and Bax levels (1.88-fold). It also countered radiation (2 Gy and 3 Gy) induced bone marrow apoptosis (1.59-fold and 1.85-fold) and micronuclei frequency (1.72-fold and 2.6-fold). SBL-1 rendered radiation protection by promoting cryptal stem cells proliferation, by regulating apoptosis, and by countering radiation induced chromosomal damage. Quercetin, Ellagic acid, Gallic acid, high contents polyphenols, tannins, and thiols detected in SBL-1 may have contributed to radiation protection by neutralization of radiation induced oxidative species, supporting stem cell proliferation and tissue regeneration. PMID:26421051

  4. Sea Buckthorn Leaf Extract Protects Jejunum and Bone Marrow of 60Cobalt-Gamma-Irradiated Mice by Regulating Apoptosis and Tissue Regeneration

    Directory of Open Access Journals (Sweden)

    Madhu Bala

    2015-01-01

    Full Text Available A single dose (30 mg/kg body weight of standardized sea buckthorn leaf extract (SBL-1, administered 30 min before whole body 60Co-gamma-irradiation (lethal dose, 10 Gy, protected >90% of mice population. The purpose of this study was to investigate the mechanism of action of SBL-1 on jejunum and bone marrow, quantify key bioactive compounds, and analyze chemical composition of SBL-1. Study with 9-week-old inbred male Swiss albino Strain ‘A’ mice demonstrated that SBL-1 treatment before 60Co-gamma-irradiation (10 Gy significantly (p<0.05 countered radiation induced decreases in jejunum crypts (1.27-fold, villi number (1.41-fold, villus height (1.25-fold, villus cellularity (2.27-fold, cryptal Paneth cells (1.89-fold, and Bcl2 level (1.54-fold. It countered radiation induced increases in cryptal apoptotic cells (1.64-fold and Bax levels (1.88-fold. It also countered radiation (2 Gy and 3 Gy induced bone marrow apoptosis (1.59-fold and 1.85-fold and micronuclei frequency (1.72-fold and 2.6-fold. SBL-1 rendered radiation protection by promoting cryptal stem cells proliferation, by regulating apoptosis, and by countering radiation induced chromosomal damage. Quercetin, Ellagic acid, Gallic acid, high contents polyphenols, tannins, and thiols detected in SBL-1 may have contributed to radiation protection by neutralization of radiation induced oxidative species, supporting stem cell proliferation and tissue regeneration.

  5. Sea Buckthorn Leaf Extract Protects Jejunum and Bone Marrow of (60)Cobalt-Gamma-Irradiated Mice by Regulating Apoptosis and Tissue Regeneration.

    Science.gov (United States)

    Bala, Madhu; Gupta, Manish; Saini, Manu; Abdin, M Z; Prasad, Jagdish

    2015-01-01

    A single dose (30 mg/kg body weight) of standardized sea buckthorn leaf extract (SBL-1), administered 30 min before whole body (60)Co-gamma-irradiation (lethal dose, 10 Gy), protected >90% of mice population. The purpose of this study was to investigate the mechanism of action of SBL-1 on jejunum and bone marrow, quantify key bioactive compounds, and analyze chemical composition of SBL-1. Study with 9-week-old inbred male Swiss albino Strain 'A' mice demonstrated that SBL-1 treatment before (60)Co-gamma-irradiation (10 Gy) significantly (p < 0.05) countered radiation induced decreases in jejunum crypts (1.27-fold), villi number (1.41-fold), villus height (1.25-fold), villus cellularity (2.27-fold), cryptal Paneth cells (1.89-fold), and Bcl2 level (1.54-fold). It countered radiation induced increases in cryptal apoptotic cells (1.64-fold) and Bax levels (1.88-fold). It also countered radiation (2 Gy and 3 Gy) induced bone marrow apoptosis (1.59-fold and 1.85-fold) and micronuclei frequency (1.72-fold and 2.6-fold). SBL-1 rendered radiation protection by promoting cryptal stem cells proliferation, by regulating apoptosis, and by countering radiation induced chromosomal damage. Quercetin, Ellagic acid, Gallic acid, high contents polyphenols, tannins, and thiols detected in SBL-1 may have contributed to radiation protection by neutralization of radiation induced oxidative species, supporting stem cell proliferation and tissue regeneration.

  6. Effect of dietary supplementation of sea buckthorn and giloe leaf meal on the body weight gain, feed conversion ratio, biochemical attributes, and meat composition of turkey poults

    Directory of Open Access Journals (Sweden)

    Aditya Sharma

    2018-01-01

    Full Text Available Aim: In the recent past, few studies have been carried out about sea buckthorn (SBT and giloe in chicken as a part of the quest for suitable alternatives to antibiotics. However, studies in turkeys are lacking. Hence, the present study was conducted to evaluate the effects of SBT and giloe leaf meal by dietary feed supplementation in turkey poults. Materials and Methods: A total of 1-day-old turkey poults (n=84 of small white variety were distributed into four dietary treatments having three replicates each with seven birds. The study was conducted in turkey poults during 0-8 weeks of age. During the experiment, the poults were fed basal ration (28% crude protein [CP], 2800 Kcal/kg ME T1, T2-basal ration was supplemented with SBT leaf meal powder at 0.5%, T3-basal ration was supplemented with giloe leaf meal powder at 0.5%, and T4-basal ration was fed along with supplementation of both SBT at 0.5% and giloe leaf meal powder at 0.5%. Results: T2 turkey poults had a significantly higher (p<0.01 body weight gain than T3 and T4 at 7th week of age. Weekly body weight gain was significantly higher (p<0.05 in T2 than T3 during 5th-8th week and 0-8th week of the growth phase. Feed conversion ratio (FCR was significantly better (p<0.01 in T2 than other treatment groups during 4th-8th week phase of growth (2.09 vs. 2.36, 2.29 and 2.31. Further, FCR was significantly better (p<0.01 in T2 group as compared to other treatment groups during 0-8th week of growth phase (1.95 vs. 2.21, 2.21 and 2.12. Plasma uric acid was found significantly increased (p<0.05 in T1 than T3 and T4, and alkaline phosphatase value was significantly higher (p<0.05 in T1 and T3 than T2. Zinc content of breast (pectoralis major muscles was significantly higher (p<0.05 in T2 and T4 as compared to T1, while ether extract (EE in thigh (ilio tibialis muscles was significantly higher (p<0.05 in T2 as compared to the other treatment groups. Conclusion: It may be concluded that

  7. Antimicrobial Effect of Escherichia Coli on Essential Oils Derived from Romanian Aromatic Plants

    Directory of Open Access Journals (Sweden)

    Şandru Daniela Maria

    2015-07-01

    Full Text Available This paper investigates the antimicrobial action of Escherichia coli ATCCR CRM-8739TM on the following essential oils: Teucrium marum, Pinus sylwestris, Thymus vulgaris, Salviae aethedaroleum, Cinnamomum aromaticum, Hippophae rhamnoides, Lavandula angustifolia, Abies alba, Zingiber officinale, Anethum graveolens, Coriandrum sativum, Origanum vulgare, extracted industrialy from romanian plants, using the diffusion disc method. The most intense activity was observed at the essential oil of Cinnamomum aromaticum (cinnamon and the mildest activity was observed at Zingiber officinale (ginger. Many of the essential oils tested exhibited moderate antimicrobial activity, as Teucrium marum, Thymus vulgaris, Hippophae rhamnoides, Lavandula angustifolia,Coriandrum sativum. The lowest antibacterial activity was exhibited on Pinus sylwestris, Salviae aethedaroleum, Zingiber officinale and Anethum graveolens.

  8. Eco-toxicological effects of two kinds of lead compounds on forest tree seed in alkaline soil.

    Science.gov (United States)

    Yang, Nan; Zhou, Fu-Rong; Wang, Jin-Xin

    2016-03-01

    In order to compare the different eco-toxicological effects of lead nitrate and lead acetate on forest tree seed, a biological incubation experiment was conducted to testify the inhibition effects of two lead compounds on rates of seed germination, root and stem elongation, and seedling fresh weight for six plants (Amaorpha fruticosa L., Robinia psedoacacia L., Pinus tabuliformis Carr., Platycladus orientalis L., Koelreuteria paniculata Laxm., Hippophae rhamnoides L.) in soil. The results indicate that the inhibition effects of the two lead compounds on the rates of root elongation of plants were greater than other indices; root elongation can possibly be used as indices to investigate the relationship between lead toxicity and plant response. The response of trees to lead toxicity varied significantly, and the order of tolerance to lead pollution was as follows: Amaorpha fruticosa L. > Platycladus orientalis L. > Koelreuteria paniculata Laxm. > Robinia psedoacacia L. > Pinus tabuliformis Carr. > Hippophae rhamnoides L. Therefore, we suggest that Amaorpha fruticosa L. and Platycladus orientalis L. be used as tolerant plants for soil phytoremediation and Hippophae rhamnoides L. as an indicative plant to diagnose the toxicity of lead pollution on soil quality. Lead nitrate and lead acetate differentially restrain seeds, with seeds being more sensitive to lead nitrate than lead acetate in the soil. Thus, the characteristics of lead compounds should be taken into full consideration to appraise its impact on the environment.

  9. Nutritional Profile of Phytococktail from Trans-Himalayan Plants

    Science.gov (United States)

    Dhar, Priyanka; Tayade, Amol B.; Kumar, Jatinder; Chaurasia, Om P.; Srivastava, Ravi B.; Singh, Shashi B.

    2013-01-01

    We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca), and roseroot (Rhodiola imbricata) from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS). Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC) with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID) with fatty acid methyl esters (FAMEs) derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA) and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs), palmitoleic acid (43.47%), oleic acid (20.89%), and linoleic acid (4.31%) were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES), and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit in the

  10. Nutritional profile of phytococktail from trans-Himalayan plants.

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    Priyanka Dhar

    Full Text Available We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides, apricot (Prunus armeniaca, and roseroot (Rhodiola imbricata from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS. Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID with fatty acid methyl esters (FAMEs derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs, palmitoleic acid (43.47%, oleic acid (20.89%, and linoleic acid (4.31% were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES, and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit

  11. Measuring and modeling stemflow by two xerophytic shrubs in the Loess Plateau: The role of dynamic canopy structure

    Science.gov (United States)

    Zhang, Y.; Li, X.; Li, W.; Shi, F.; Wu, H.; WU, X.; Pei, T.

    2016-12-01

    Stemflow plays an important role in hydrological processes in dryland shrubs, but it still remains poorly understood, especially regarding the effects of dynamic canopy structure on stemflow. This study aimed to measure and model the stemflow of two dominant xerophytic shrub (Hippophae rhamnoides and Spiraea pubescens) communities and to identify the key controlling factors of stemflow yield. We quantified and scaled-up stemflow from branches and leaves to stand levels. Correlations and stepwise regression analysis between stemflow and meteorological and biological factors indicated that at branch level, the rainfall amount and the branch diameter were the best variables for modelling and predicting stemflow for Hippophae rhamnoides, while the rainfall amount and the aboveground biomass were the best variables for modelling and predicting stemflow for Spiraea pubescens. At stand level, the stemflow yield is mostly affected by rainfall amount and leaf area index for both shrubs. The stemflow fluxes account for 3.5±0.9% of incident rainfall for H. rhamnoides community and 9.4±2.1% for S. pubescens community, respectively. The differences in percentages of stemflow between the two shrub communities was attributed to differences in canopy structures and water storage capacities. This evaluation of the effects of canopy structure dynamics on stemflow, and of the developed model, provided a better understanding of the effect of the canopy structure on the water cycles in dryland shrub ecosystems.

  12. In vitro antioxidant profiling of seabuckthorn varieties and their adaptogenic response to high altitude-induced stress

    Science.gov (United States)

    Sharma, Priyanka; Suryakumar, Geetha; Singh, Virendra; Misra, Kshipra; Singh, Shashi Bala

    2015-08-01

    In the past few years, seabuckthorn plants have gained special attention due to their ability to grow in the harshest of the environment. This adaptability may be contributed by various antioxidants present in the plants besides other morphological adaptation. As in vivo studies cannot be justified without in vitro studies, the present investigation carried out evaluation of both in vitro and in vivo antioxidant potentials of aqueous and alcoholic extracts of the leaves of Hippophae salicifolia (HS) and Hippophae rhamnoides mongolica (HRM) in comparison with Hippophae rhamnoides turkestanica (HRT). The results had clearly depicted that in vitro antioxidant potential of the extracts was responsible for the in vivo adaptogenic performance in animals during cold and hypoxia exposure under restraint stress. Total phenolic content (TPC), total flavonoid content (TFC), total protein content, and antioxidant potential were determined. For adaptogenic studies, rats with oral drug supplementation were exposed to Cold-hypoxia-restraint (C-H-R) stresses-induced hypothermia, as a measure of endurance. Aqueous extracts of HS showed maximum (99 %) resistance compared to HRT (81 %) and HRM (29 %). The levels of biochemical parameters such as malondialdehyde (MDA), reactive oxygen species (ROS), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione (GSH/GSSG), and catalase (CAT) in blood samples also revealed that the aqueous leaf extract of HS has better antioxidant and adaptogenic potential compared to HRM.

  13. Synergy between antibiotics and natural agents results in increased antimicrobial activity against Staphylococcus epidermidis.

    Science.gov (United States)

    Abidi, Syed Hani; Ahmed, Khalid; Sherwani, Sikander Khan; Kazmi, Shahana Urooj

    2015-09-27

    Staphylococcus epidermidis is one of the most frequent causes of biofilm-associated infections on indwelling medical devices. With the emergence of methicillin-resistant S. epidermidis (MRSE), there is an urgent need to discover novel active agents against a range of Gram-positive pathogens. We screened the clinical isolates of S. epidermidis for susceptibility/resistance against commonly prescribed antibiotics. Furthermore, we tested some natural agents alone and in combination with antibiotics to find possible synergistic antimicrobial effects. S. epidermidis clinical isolates were screened for susceptibility/resistance against vancomycin, erythromycin, tetracycline, chloramphenicol, ampicillin, ofloxacin, cephalexin, and gentamicin using the Kirby-Bauer disk diffusion method. The antimicrobial potential of Camellia sinensis, Juglans regia, and Hippophae rhamnoides alone and in combination with antibiotics were examined using the disk diffusion method, where the antimicrobial potential activity was measured in terms of formation of zones of inhibition. Most S. epidermidis isolates were found to be resistant to one or more antibiotics. Gentamycin and ofloxacin were found to be the most effective antibiotics against S. epidermidis isolates. Extracts of Hippophae rhamnoides, Juglans regia, and Camellia sinensis were found to be equally effective against S. epidermidis isolates. In combination with antibiotics, these extracts exhibited appreciable synergistic activity; the highest synergistic activity was observed with erythromycin and cephalexin. In the case of cephalexin, a reversion in resistance was observed. The plant extracts used in the study exhibited additive and synergistic antibacterial activity against S. epidermidis, hence providing an effective alternative to deal with the problem of multidrug resistance.

  14. Contrasting water use pattern of introduced and native plants in an alpine desert ecosystem, Northeast Qinghai–Tibet Plateau, China

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Huawu, E-mail: wuhuawu416@163.com [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Li, Xiao-Yan, E-mail: xyli@bnu.edu.cn [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Jiang, Zhiyun; Chen, Huiying; Zhang, Cicheng; Xiao, Xiong [College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China)

    2016-01-15

    Plant water use patterns reflect the complex interactions between different functional types and environmental conditions in water-limited ecosystems. However, the mechanisms underlying the water use patterns of plants in the alpine desert of the Qinghai–Tibet Plateau remain poorly understood. This study investigated seasonal variations in the water sources of herbs (Carex moorcroftii, Astragalus adsurgens) and shrubs (Artemisia oxycephala, Hippophae rhamnoides) using stable oxygen-18 isotope methods. The results indicated that the native herbs (C. moorcroftii, A. adsurgens) and one of the shrubs (A. oxycephala) mainly relied on water from the shallow layer (0–30 cm) throughout the growing season, while the introduced shrub (H. rhamnoides) showed plasticity in switching between water from shallow and deep soil layers depending on soil water availability. All studied plants primarily depended on water from shallow soil layers early in the season. The differences of water use patterns between the introduced and native plants are closely linked with the range of active root zones when competing for water. Our findings will facilitate the mechanistic understanding of plant–soil–water relations in alpine desert ecosystems and provide information for screening introduced species for sand fixation. - Highlights: • Stable oxygen-18 in soil water experienced great evaporation enrichment. • H. rhamnoides experiences a flexible plasticity to switch between shallow and deep soil water. • Native plants mostly relied on shallow and middle soil water. • Water-use patterns by introduced-native plants are controlled by root characteristics.

  15. Isorhamnetin Attenuates Staphylococcus aureus-Induced Lung Cell Injury by Inhibiting Alpha-Hemolysin Expression.

    Science.gov (United States)

    Jiang, Lanxiang; Li, Hongen; Wang, Laiying; Song, Zexin; Shi, Lei; Li, Wenhua; Deng, Xuming; Wang, Jianfeng

    2016-03-01

    Staphylococcus aureus, like other gram-positive pathogens, has evolved a large repertoire of virulence factors as a powerful weapon to subvert the host immune system, among which alpha-hemolysin (Hla), a secreted pore-forming cytotoxin, plays a preeminent role. We observed a concentration-dependent reduction in Hla production by S. aureus in the presence of sub-inhibitory concentrations of isorhamnetin, a flavonoid from the fruits of Hippophae rhamnoides L., which has little antibacterial activity. We further evaluate the effect of isorhamnetin on the transcription of the Hla-encoding gene hla and RNAIII, an effector molecule in the agr system. Isorhamnetin significantly down-regulated RNAIII expression and subsequently inhibited hla transcription. In a co-culture of S. aureus and lung cells, topical isorhamnetin treatment protected against S. aureus-induced cell injury. Isorhamnetin may represent a leading compound for the development of anti-virulence drugs against S. aureus infections.

  16. [Distribution of fine root biomass of main planting tree species in Loess Plateau, China].

    Science.gov (United States)

    Jian, Sheng-Qi; Zhao, Chuan-Yan; Fang, Shu-Min; Yu, Kai

    2014-07-01

    The distribution of fine roots of Pinus tabuliformis, Populus tomentosa, Prunus armeniaca, Robinia pseudoacacia, Hippophae rhamnoides, and Caragana korshinskii was investigated by using soil core method and the fine root was defined as root with diameter less than 2 mm. The soil moisture and soil properties were measured. The results showed that in the horizontal direction, the distribution of fine root biomass of P. tabuliformis presented a conic curve, and the fine root biomass of the other species expressed logarithm correlation. Radial roots developed, the fine root biomass were concentrated within the scope of the 2-3 times crown, indicating that trees extended their roots laterally to seek water farther from the tree. In the vertical direction, the fine root biomass decreased with the increasing soil depth. Fine root biomass had significant negative correlation with soil water content and bulk density, while significant positive correlation with organic matter and total N contents.

  17. Sap flow in response to rainfall pulses for two shrub species in the semiarid Chinese Loess Plateau

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    Jian Shengqi

    2016-06-01

    Full Text Available Rainfall pulses can significantly drive the evolution of the structure and function of semiarid ecosystems, and understanding the mechanisms that underlie the response of semiarid plants to rainfall is the key to understanding the responses of semi–arid ecosystems to global climatic change. We measured sap flow in the branches and stems of shrubs (Caragana korshinskii Kom. and Hippophae rhamnoides Linn. using sap flow gauges, and studied the response of sap flow density to rainfall pulses using the “threshold–delay” model in the Chinese Loess Plateau. The results showed that the sap flow began about 1 h earlier, and increased twofold after rainfall, compared to its pre-rainfall value. The sap flow increased significantly with increasing rainfall classes, then gradually decreased. The response of sap flow was different among rainfall, species, position (branch and stem during the pulse period, and the interactive effects also differed significantly (P < 0.0001. The response pattern followed the threshold–delay model, with lower rainfall thresholds of 5.2, 5.5 mm and 0.7, 0.8 mm of stem and branch for C. korshinskii and H. rhamnoides, demonstrating the importance of small rainfall events for plant growth and survival in semi–arid regions.

  18. [Energy and nutrient characteristics of Carex lanceolata in Ziwuling of Loess Plateu].

    Science.gov (United States)

    Chen, Mei-ling; Shangguan, Zhou-ping

    2008-01-01

    The study on the energy and nutrient characteristics of Carex lanceolata, a dominant companion species in different plant communities in Ziwuling forest area of Loess Plateau showed that the ash free caloric values of the aboveground and underground parts of the species tended to decline in the communities experienced different successional stages. The aboveground part of C. lanceolata had the highest ash free caloric value in Sophora viciifolia community and the lowest in Hippophae rhamnoides community. The underground part of C. lanceolata had the highest ash free caloric value in S. viciifolia community and the lowest in Quercus liaotungensis community. The aboveground part of C. lanceolata had a remarkably higher caloric value than its underground part, and the underground part appeared to extremely significantly differ in gross and ash-free caloric values among different communities. The caloric value of C. lanceolata varied greatly in the communities going through their early successional stages (S. viciifolia, H. rhamnoides, Populus davidiana and Betula platyphylla communities), and less greatly in the communities experiencing their later successional stages (Pinus tabulaeformis and Q. liaotungensis communities). There was a significant positive correlation between the gross caloric value and the C/N ratio in the underground part of C. lanceolata.

  19. Response pattern of amino compounds in phloem and xylem of trees to soil drought depends on drought intensity and root symbiosis.

    Science.gov (United States)

    Liu, X-P; Gong, C-M; Fan, Y-Y; Eiblmeier, M; Zhao, Z; Han, G; Rennenberg, H

    2013-01-01

    This study aimed to identify drought-mediated differences in amino nitrogen (N) composition and content of xylem and phloem in trees having different symbiotic N(2)-fixing bacteria. Under controlled water availability, 1-year-old seedlings of Robinia pseudoacacia (nodules with Rhizobium), Hippophae rhamnoides (symbiosis with Frankia) and Buddleja alternifolia (no such root symbiosis) were exposed to control, medium drought and severe drought, corresponding soil water content of 70-75%, 45-50% and 30-35% of field capacity, respectively. Composition and content of amino compounds in xylem sap and phloem exudates were analysed as a measure of N nutrition. Drought strongly reduced biomass accumulation in all species, but amino N content in xylem and phloem remained unaffected only in R. pseudoacacia. In H. rhamnoides and B. alternifolia, amino N in phloem remained constant, but increased in xylem of both species in response to drought. There were differences in composition of amino compounds in xylem and phloem of the three species in response to drought. Proline concentrations in long-distance transport pathways of all three species were very low, below the limit of detection in phloem of H. rhamnoides and in phloem and xylem of B. alternifolia. Apparently, drought-mediated changes in N composition were much more connected with species-specific changes in C:N ratios. Irrespective of soil water content, the two species with root symbioses did not show similar features for the different types of symbiosis, neither in N composition nor in N content. There was no immediate correlation between symbiotic N fixation and drought-mediated changes in amino N in the transport pathways. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

  20. An experience and contemporary status of forest recultivation in Kuzbass

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    V. I. Ufimtsev

    2017-08-01

    Full Text Available Reforestation is the main direction of rehabilitation of the degraded lands by the coal industry. The substratum of dumps of the coal industry is characterized by sharp deficiency of elements and the expressed xeromorphy. During more than 40 years on the dumps were created over 15 thousand ha of wood plantings, the agrotechnological and scientific foundation of forest recultivation is laid. Biological features of several tens types are studied, their suitability for the purposes of afforestation of dumps is estimated. Scots pine Pinus sylvestris L., a common sea-buckthorn Hippophaë rhamnoídes L. and a silver birch Betula pendula Tristis appeared certainly suitable, 10 more types are recognized as the perspective. In 1989 and 2005 under the leadership of L. P. Barannik are prepared and approved for application at the regional level of the recommendation about reforestation. Now experiments on selection of types proceed, work on optimization of technologies of forest recultivation taking into account an assessment of the created plantings of the I–II class of age is conducted. The main square of reforestation, about 11 thousand hectares, the scots pine which is capable to grow on the highest classes of site class without decrease in growth processes in the senior age groups borrows. Pure sea-buckthorn plantings are created on the area about 3 thousand hectares, they are used by the population as berry-pickers, the ecological value of a sea-buckthorn consists in fast development of a surface of dumps, unpretentiousness. Joint landings of these two tree species are widely used. The birch hung, along with a birch fluffy, is presented, mainly, in the form of natural renewal. In recent years, in connection with reduction of nurseries on cultivation of seedlings of a pine, the birch began to be used actively at artificial afforestation of dumps, especially in urban areas. The main problems of forest recultivation are: non-selective formation of

  1. Gene pool of less widely spread fruit tree species

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    Vojtěch Řezníček

    2004-01-01

    Full Text Available Within the gene pool collected at the Department of Breeding and Propagation of Garden Plants of the Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, in Lednice we established experimental plots with some selected less known tree species - quince (Cydonia oblonga Mill., sea buckthorn (Hippophäe rhamnoides L., Cornelian cherry (Cornus mas L. and honeysuckle (Lonicera caerulea subsp. edulis Turcy. ex Freyn.. The experimental plots were established in successive steps according to the availability of planting material and using conventional methods of cultivation. Evaluations are focused on selected growth parameters, phenology and commercial use of the fruit.The evaluations of the crown of quince showed differences in the size and shape. The variety Hemus had the largest crown volume (5.70 m3; the variety Blanár gave the highest harvest yields. The sea buckthorn varieties Polmix, Dar Katuni and Novosť Altaja produced the longest increments. The average weight of the fruit of the variety Leicora was 0.74 g. The varieties of Cornelian cherry also differed in the growth parameters; the highest shrubs were those of the variety Vyšegorodskij, which also produced the largest fruit – the average weight of the fruit was 4.85 g. The initial growth of selected varieties and genotypes of honeysuckle is different when compared to the fruit-bearing shrubs. Harvest data are in direct proportion to the size of the shrub. Fruit harvest began in mid-May and vegetation ended on 15 October.

  2. Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection.

    Science.gov (United States)

    Wang, Wei; Lin, Ping; Ma, Lihong; Xu, Kaixuan; Lin, Xiuli

    2016-04-01

    Flavonoids are important active ingredients in many traditional Chinese medicines. In this paper, capillary electrophoresis with amperometric detection was employed to separate and detect eight flavonoids, rutin, quercetrin, quercetin, kaempferol, kaempferide, catechin, apigenin, and luteolin, in a home-made capillary electrophoresis device. Under the separation voltage of 2000 V, the eight flavonoids could be completely separated within 33 min in 18 mM borax running buffer at pH 10.2. Good linear relationships were obtained for all analytes and the detection limits for flavonoids ranged from 0.46 to 0.85 μM. Then, the method was applied to separate and determine the flavonoids in three traditional Chinese medicines, hippophae rhamnoides, hypericum perforatum, and cacumen platycladi. Finally, rutin, kaempferol, quercetin, and quercetrin were discovered in these medicines and the concentrations ranged from 0.28 to 9.94 mg/g. The recoveries of flavonoids ranged from 84.7 to 113%, which showed the high reliability of this method. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. HOST PLANTS AND CLIMATIC PREFERENCES OF THE INVASIVE SPECIES METCALFA PRUINOSA (SAY 1830 (HEMIPTERA: FLATIDAE IN SOME PLACES FROM SOUTHERN ROMANIA

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    Daniela Barbuceanu

    2015-12-01

    Full Text Available Observations carried out in May-September 2015 in two sites of Southern Romania reveal a rich spectrum of host plants for Metcalfa pruinosa, which consists of 204 species in 56 families. The species it is noticed on weeds and cultivated plants. The remarkable polyphagia of this species, the lack of natural enemies, and the climatic conditions of 2015 - warm and dry summer, had lead to an invasion of M. pruinosa, in the researched areas; the highest numerical abundances are noticed in shady habitats. Furthermore, on herbs, such as Levisticum officinale, Artemisia dracunculus, Ocimum basilicum, Mentha spp., usually avoided by pests, were observed colonies of the species. It is recorded high numerical abundance on fruit trees and shrubs: Hippophaë rhamnoides, Juglans regia, Prunus cerasus, Vitis vinifera, Rubus idaeus. The harmful effect occurs on apple trees Romus 1 variety as a result of the association with another pest of American origin, Eriosoma lanigerum, situation that favors the attack of the Erwinia amylovora bacteria, causing the collapse of the tree. It is found that altitudes higher than 200 m do not represent a limitative factor in the spreading of species, one of the investigated sites being located at 304 m altitude.

  4. Micromonospora is a normal occupant of actinorhizal nodules.

    Science.gov (United States)

    Carro, Lorena; Pujic, Petar; Trujillo, Martha E; Normand, Philippe

    2013-11-01

    Actinorhizal plants have been found in eight genera belonging to three orders (Fagales, Rosales and Cucurbitales). These all bear root nodules inhabited by bacteria identified as the nitrogen-fixing actinobacterium Frankia. These nodules all have a peripheral cortex with enlarged cells filled with Frankia hyphae and vesicles. Isolation in pure culture has been notoriously difficult, due in a large part to the growth of fast-growing contaminants where, it was later found, Frankia was slow-growing. Many of these contaminants, which were later found to be Micromonospora, were obtained from Casuarina and Coriaria. Our study was aimed at determining if Micromonospora were also present in other actinorhizal plants. Nodules from Alnus glutinosa, Alnus viridis, Coriaria myrtifolia, Elaeagnus x ebbingei, Hippophae rhamnoides, Myrica gale and Morella pensylvanica were tested and were all found to contain Micromonospora isolates. These were found to belong to mainly three species: Micromonospora lupini, Micromonospora coriariae and Micromonospora saelicesensis. Micromonospora isolates were found to inhibit some Frankia strains and to be innocuous to other strains.

  5. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

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    Saima Jadoon

    2015-01-01

    Full Text Available The exposure to ultraviolet radiations (UVR is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS, leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  6. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity.

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Bin Asad, Muhammad Hassham Hassan; Akram, Muhammad Rouf; Khan, Abida Kalsoom; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  7. Genetic structure in the seabuckthorn carpenter moth (Holcocerus hippophaecolus in China: the role of outbreak events, geographical and host factors.

    Directory of Open Access Journals (Sweden)

    Jing Tao

    Full Text Available Understanding factors responsible for structuring genetic diversity is of fundamental importance in evolutionary biology. The seabuckthorn carpenter moth (Holcocerus hippophaecolus Hua is a native species throughout the north of China and is considered the main threat to seabuckthorn, Hippophae rhamnoides L. We assessed the influence of outbreaks, environmental factors and host species in shaping the genetic variation and structure of H. hippophaecolus by using Amplified Fragment Length Polymorphism (AFLP markers. We rejected the hypothesis that outbreak-associated genetic divergence exist, as evidenced by genetic clusters containing a combination of populations from historical outbreak areas, as well as non-outbreak areas. Although a small number of markers (4 of 933 loci were identified as candidates under selection in response to population densities. H. hippophaecolus also did not follow an isolation-by-distance pattern. We rejected the hypothesis that outbreak and drought events were driving the genetic structure of H. hippophaecolus. Rather, the genetic structure appears to be influenced by various confounding bio-geographical factors. There were detectable genetic differences between H. hippophaecolus occupying different host trees from within the same geographic location. Host-associated genetic divergence should be confirmed by further investigation.

  8. Isolation and analysis of vitamin B12 from plant samples.

    Science.gov (United States)

    Nakos, M; Pepelanova, I; Beutel, S; Krings, U; Berger, R G; Scheper, T

    2017-02-01

    Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n=6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37μg/100g dry weight), in Elymus (26μg/100g dry weight) and in Inula helenium (11μg/100g dry weight). Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. The Dynamic Trend of Soil Water Content in Artificial Forests on the Loess Plateau, China

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2016-10-01

    Full Text Available Extensive vegetation restoration projects have been widely implemented on the Loess Plateau, China, since 1998. In addition, increasing attention has been paid to the influence of revegetation on soil water. However, the response of the soil water content (SWC to vegetation construction and management has not been adequately studied. In this study, three types of typical artificial vegetation on level bench land were selected, including Pinus tabulaeformis Carr., Prunus sibirica L., and Hippophae rhamnoides Linn., with the natural grassland used as a control group in Wuqi County. The 0–160 cm SWC was monitored biweekly from August 2010 to June 2013 using a portable time domain reflectometry system. The serial autocorrelation test, Mann–Kendall trend test, and prewhitening Mann–Kendall test were employed to systematically analyze the trends in soil water dynamics. The results show that the SWC of the three selected artificial forests/shrub had a significant accumulation process in the 0–160 cm profile during the monitoring period, whereas such an increasing tendency was not observed for natural grassland. Furthermore, the greatest responses were observed in the Pinus tabulaeformis Carr. plantation.

  10. Effects of trampling on morphological and mechanical traits of dryland shrub species do not depend on water availability.

    Directory of Open Access Journals (Sweden)

    Liang Xu

    Full Text Available In semiarid drylands water shortage and trampling by large herbivores are two factors limiting plant growth and distribution. Trampling can strongly affect plant performance, but little is known about responses of morphological and mechanical traits of woody plants to trampling and their possible interaction with water availability. Seedlings of four shrubs (Caragana intermedia, Cynanchum komarovi, Hedysarum laeve and Hippophae rhamnoides common in the semiarid Mu Us Sandland were grown at 4% and 10% soil water content and exposed to either simulated trampling or not. Growth, morphological and mechanical traits were measured. Trampling decreased vertical height and increased basal diameter and stem resistance to bending and rupture (as indicated by the increased minimum bend and break force in all species. Increasing water availability increased biomass, stem length, basal diameter, leaf thickness and rigidity of stems in all species except C. komarovii. However, there were no interactive effects of trampling and water content on any of these traits among species except for minimum bend force and the ratio between stem resistance to rupture and bending. Overall shrub species have a high degree of trampling resistance by morphological and mechanical modifications, and the effects of trampling do not depend on water availability. However, the increasing water availability can also affect trade-off between stem strength and flexibility caused by trampling, which differs among species. Water plays an important role not only in growth but also in trampling adaptation in drylands.

  11. Possibilities of cultivating ornamental trees and shrubs under conditions of air pollution with oxides of sulfur

    Energy Technology Data Exchange (ETDEWEB)

    Bialobok, S.; Bartkowiak, S.; Rachwal, L.

    1974-01-01

    The field work conducted has shown that high concentrations of SO/sub 2/ in the air can be withstood by the following trees and shrubs. Trees: Acer campestris, A. platanoides, Ailanthus altissima, Aesculus hippocastanum, Morus alba, Platanus acerifolia, Pinus strobur, P. nigra, Populus Berolinensis, P. candicans, P. Hybr. 27, P. Marilandica, P. simonii, P. Serotina, Quercus robus, Robinia pseudoacacia. Shrubs: Caragana arborescens, Crataegus oxyacantha, C. monogyna, Cerasus mehaleb, Forsythia/most of the species and varieties/, Ligustrum vulgare, Philadelphus coronaria, Ptelea trifoliata, Sambucus nigra, Salix caprea, Sorbaria sorbifolia, Sorbus aucuparia, Taxus baccata. For the selection of trees and shrubs in the laboratory, high concentrations of SO/sub 2/ were used (60-150 ppm for a period of 10 minutes). Experiments were conducted on cut shrubs kept in the gas chambers. In order to estimate the degree of their injury, they were transferred to a shaded greenhouse. A concentration of 65 ppm of SO/sub 2/ could be withstood by the following Forsythias: Forsythia intermedia Primulina, F. Densiflora, F. Spectabilis, F. giraldina, F. suspensa, F. koreana, F. ovata, F. japonica and Hippophae rhamnoides. A concentration of 130 ppm could be withstood only by F. intermedia Vitelina. A similarly high concentration of SO/sub 2/ could be withstood by shoots and leaves of Ailanthus girladii Duclouxii and by Platanus acerifolia. From among the lilacs Syringa pekinensis and S. amurensis proved resistant to high concentrations of SO/sub 2/.

  12. Isorhamnetin protects against cardiac hypertrophy through blocking PI3K-AKT pathway.

    Science.gov (United States)

    Gao, Lu; Yao, Rui; Liu, Yuzhou; Wang, Zheng; Huang, Zhen; Du, Binbin; Zhang, Dianhong; Wu, Leiming; Xiao, Lili; Zhang, Yanzhou

    2017-05-01

    Isorhamnetin, a flavonoid compound extracted from the Chinese herb Hippophae rhamnoides L., is well known for its anti-inflammatory, anti-oxidative, anti-adipogenic, anti-proliferative, and anti-tumor activities. However, the role of isorhamnetin in cardiac hypertrophy has not been reported. The aims of the present study were to find whether isorhamnetin could alleviate cardiac hypertrophy and to define the underlying molecular mechanisms. Here, we investigated the effects of isorhamnetin (100 mg/kg/day) on cardiac hypertrophy induced by aortic banding in mice. Cardiac hypertrophy was evaluated by echocardiographic, hemodynamic, pathological, and molecular analyses. Our data demonstrated that isorhamnetin could inhibit cardiac hypertrophy and fibrosis 8 weeks after aortic banding. The results further revealed that the effect of isorhamnetin on cardiac hypertrophy was mediated by blocking the activation of phosphatidylinositol 3-kinase-AKT signaling pathway. In vitro studies performed in neonatal rat cardiomyocytes confirmed that isorhamnetin could attenuate cardiomyocyte hypertrophy induced by angiotensin II, which was associated with phosphatidylinositol 3-kinase-AKT signaling pathway. In conclusion, these data indicate for the first time that isorhamnetin has protective potential for targeting cardiac hypertrophy by blocking the phosphatidylinositol 3-kinase-AKT signaling pathway. Thus, our study suggests that isorhamnetin may represent a potential therapeutic strategy for the treatment of cardiac hypertrophy and heart failure.

  13. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Science.gov (United States)

    Karim, Sabiha; Asad, Muhammad Hassham Hassan Bin; Kalsoom Khan, Abida; Malik, Arif; Chen, Chunye

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed. PMID:26448818

  14. [Dynamic changes of soil microbial biomass in the restoration process of shrub plantations in loess hilly area].

    Science.gov (United States)

    Xue, Sha; Liu, Guo-Bin; Dai, Quan-Hou; Li, Xiao-Li; Wu, Rui-Jun

    2008-03-01

    By the method of spatiotemporal substitution and taking a slope farmland and a natural Platycladus orientailis L. forest as the controls, this paper studied the dynamic changes of soil microbial biomass, microbial respiration, metabolic quotient (qCO2) and physicochemical properties under the Caragana korshinkii and Hippophae rhamnoides plantations with different restoration age in loess hilly area. The results showed that with the increasing restoration age of the shrubs, soil physicochemical properties improved obviously, and soil microbial biomass had a significant increase. After 7 years restoration of C. korshinkii plantation, soil microbial biomass C increased apparently, compared with that in farmland, and the increments after each 5-7 years were all significant. Microbial biomass N and P had no significant increase in the first 13 years but kept relatively stable in the 20-30 years restoration of C. korshinkii plantation, and were significantly higher than those in farmland but lower than those under P. orientailis plantation after 30 years restoration of C. korshinkii plantation. Soil microbial respiration was enhanced with the increasing restoration age of the shrubs, with the peak in the 20-25 years restoration. After then, it decreased rapidly, and bottomed out in the 30 years restoration. qCO2 was significantly higher under P. orientailis plantation than in farmland in the early periods of shrubs restoration, and then decreased rapidly. After 30 years restoration of C. korshinkii plantation, qCO2 was lower than that in farmland but still much higher than that under P. orientailis plantation. Different shrub plantations had different effects on soil properties. H. rhamnoides with the same restoration age of C. korshinkii contributed more to the increase of soil microbial biomass and respiration. There were significant correlations between the restoration age of test shrub plantations and the microbial biomass, qCO2, and physicochemical properties of

  15. Land-Use Conversion Changes the Multifractal Features of Particle-Size Distribution on the Loess Plateau of China.

    Science.gov (United States)

    Sun, Caili; Liu, Guobin; Xue, Sha

    2016-08-05

    Analyzing the dynamics of soil particle-size distributions (PSDs), soil nutrients, and erodibility are very important for understanding the changes of soil structure and quality after long-term land-use conversion. We applied multifractal Rényi spectra (Dq) and singularity spectra (f(α)) to characterize PSDs 35 years after conversions from cropland to shrubland with Caragana microphylla (shrubland I), shrubland with Hippophae rhamnoides (shrubland II), forested land, and grassland on the Loess Plateau of China. Multifractal parameters (capacity dimension (D₀), entropy dimension (D₁), D₁/D₀, correlation dimension (D₂), and Hölder exponent of order zero (α₀)) were used to analyze the changes of PSDs. Dq and f(α) characterized the PSDs well and sensitively represented the changes in PSDs after conversion. All types of land-use conversion significantly improved the properties of the topsoil (0-10 cm), but the effect of shrubland I and even forested land decreased with depth. All types of land-use conversion significantly increased D₁ and D₂ in the topsoil, and D₁ and D₂ in the 10-50 cm layers of shrubland II, forested land, and grassland and D₁ in the 50-100 cm layers of shrubland II were significantly higher relative to the control. Both D₁ and D₂ were positively correlated with the contents of soil nutrients and fine particles and were negatively correlated with soil erosion, indicating that D₁ and D₂ were potential indices for quantifying changes in soil properties and erosion. In conclusion, all types of land-use conversion significantly improved the conditions of the topsoil, but conversion from cropland to shrubland II, forested land, and grassland, especially shrubland II and grassland, were more effective for improving soil conditions in deeper layers.

  16. Soil characterization and differential patterns of heavy metal accumulation in woody plants grown in coal gangue wastelands in Shaanxi, China.

    Science.gov (United States)

    Yakun, Shi; Xingmin, Mu; Kairong, Li; Hongbo, Shao

    2016-07-01

    Soil contamination by heavy metals in coal mine wastelands is a significant environmental issue in most developing countries. The purpose of this study is to evaluate contamination characteristics in the coal mine wastelands of Sanlidong coal mine, Tongchuan, China. To achieve this goal, we conducted field sampling work, followed by further analysis of the properties of soil contamination and accumulation characteristics in woody plants. At this site, the pH value ranged from 4.41 to 7.88, and the nutrient content of the soil rose gradually with the time after deposition due to the weathering effect improving the soil quality. Meanwhile, the levels of Cd, Cr, Cu, Ni, and Zn gradually decreased with the passage time. Generally, heavy metal contamination was found to be more serious in the discharge refuse area, with Cd contamination at moderate or heavy levels; Ni, Zn, and Cu contamination at light levels; and with no Cr contamination. The geoaccumulation index (I geo) was highest for Cd (2.38-3.14), followed by Ni, Zn, Cu, and Cr. Heavy metals accumulated on the lower slopes and spread to the surrounding areas via hydrodynamic effects and wind. According to transfer and enrichment coefficient analyses, Robinia pseudoacacia, Ulmus pumila, and Hippophae rhamnoides with considerable biomass could be used as pollution-resistant tree species for vegetation restoration. This study provided a theoretical basis for the restoration of the ecological environment in the mining area. This report described a link between heavy metal contamination of soils and growth dynamics of woody plants in China.

  17. Phytochemical characterization and evaluation of anticataract potential of seabuckthorn leaf extract.

    Science.gov (United States)

    Dubey, Suchita; Deep, Prakash; Singh, Amrit K

    2016-03-01

    This study was undertaken to carry out phytochemical characterization of aqueous extract of Seabuckthorn (SBT, Hippophae rhamnoides L.) leaves and evaluation of its therapeutic role in oxidative stress-induced cataract in isolated goat lenses using Vit. E as reference compound. A total of 42 goat eye lenses were used in the present study. Seabuckthorn leaf extract was characterized by total phenol content estimation and HPLC analysis of quercetin and catechin. Further, cataract was induced in goat lenses using hydrogen peroxide (H2O2) and anticataract activity was evaluated using the extract in the dose range of 100, 200, 500, and 1000 μg/mL concentrations through estimation of biochemical markers such as superoxide dismutase (SOD), reduced glutathione (GSH), and malonaldehyde (MDA). The results of the phytochemical characterization showed the total phenol content of the extract to be 365 mg/g in terms of gallic acid equivalents. Quercetin and catechin were estimated to be 0.01 and 0.12% w/w, respectively. In biochemical analysis, H2O2 introduction resulted in a decrease in SOD (approximately 85%) and GSH (approximately 63%) contents and an increase in MDA content (approximately 300%). The decreased levels of SOD and GSH were significantly restored in experimental groups receiving 500 and 1000 1g/mL of SBT extract. All the experimental groups showed significantly reduced MDA level in all the doses. Aqueous extract of SBT leaves showed the potential to delay onset and/or progression of cataract, at least during in vitro conditions. Results indicate the possibilities of evaluating this extract for its use as anticataract agent during in vivo conditions. © 2015 American College of Veterinary Ophthalmologists.

  18. HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas

    Directory of Open Access Journals (Sweden)

    Raluca Maria Pop

    2013-11-01

    Full Text Available . High performance liquid chromatography (HPLC with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: Taraxacum officinalis (1, Cynara scolimus (2, Silybum marianum (3, Hypericum perforatum (4,  Chelidonium majus (5, Lycopodium clavatum (6 and  Hippophae rhamnoides (7  leaves and fruits.  Two products (A and B were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol were analyzed using HPLC-UV for their phenolics’ fingerprint and composition. The qualitative (untargeted analysis and quantitative (targeted analysis results were further compared using Principal Component Analysis (PCA in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were trans-cinnamic, chlorogenic, caffeic and p-coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids. In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.

  19. [Effects of mixed decomposition of Populus simonii and other tree species leaf litters on soil properties in Loess Plateau].

    Science.gov (United States)

    Li, Qian; Liu, Zeng-Wen; Du, Liang-Zhen

    2012-03-01

    In this study, the leaf litters of Populus simonii and other 11 tree species were put into soil separately or in mixture after grinding, and incubated in laboratory to analyze the effects of their decomposition on soil properties and the interactions between the litters decomposition. The decomposition of each kind of the leaf litters in soil increased the soil urease, dehydrogenase, and phosphatase activities and the soil organic matter and available N contents markedly, but had greater differences in the effects on the soil available P content and CEC. The decomposition of the leaf litters of Caragana microphylla and of Amorpha fruticosa showed obvious effects in improving soil properties. The decomposition of the mixed leaf litters of P. simonii and Pinus tabulaeformis, Platycladus orientalis, Robinia pseudoacacia, or Ulmus pumila showed interactive promotion effects on the abundance of soil microbes, and that of the mixed leaf litters of P. simonii and P. orientalis or C. microphylla showed interactive promotion effects on the soil organic matter, available P, and available K contents and soil CEC but interactive inhibition effects on the activities of most of the soil enzymes tested. The decomposition of the mixed leaf litters of P. simonii and Larix principis-rupprechtii showed interactive promotion effects on the activities of most of the soil enzymes and soil nutrient contents, while that of the mixed leaf litters of P. simonii and P. sylvestris var. mongolica showed interactive inhibition effects. Overall, the decomposition of the mixed leaf litters of P. simo- nii and U. pumila, P. tabulaeformis, L. principis-rupprechtii, or R. pseudoacacia could improve soil quality, but the mixed leaf litters of P. simonii and P. orientalis, C. microphylla, P. sylvestris var. mongolica, Hippophae rhamnoides, or A. fruticosa showed an interactive inhibition effect during their decomposition.

  20. Land-Use Conversion Changes the Multifractal Features of Particle-Size Distribution on the Loess Plateau of China

    Science.gov (United States)

    Sun, Caili; Liu, Guobin; Xue, Sha

    2016-01-01

    Analyzing the dynamics of soil particle-size distributions (PSDs), soil nutrients, and erodibility are very important for understanding the changes of soil structure and quality after long-term land-use conversion. We applied multifractal Rényi spectra (Dq) and singularity spectra (f(α)) to characterize PSDs 35 years after conversions from cropland to shrubland with Caragana microphylla (shrubland I), shrubland with Hippophae rhamnoides (shrubland II), forested land, and grassland on the Loess Plateau of China. Multifractal parameters (capacity dimension (D0), entropy dimension (D1), D1/D0, correlation dimension (D2), and Hölder exponent of order zero (α0)) were used to analyze the changes of PSDs. Dq and f(α) characterized the PSDs well and sensitively represented the changes in PSDs after conversion. All types of land-use conversion significantly improved the properties of the topsoil (0–10 cm), but the effect of shrubland I and even forested land decreased with depth. All types of land-use conversion significantly increased D1 and D2 in the topsoil, and D1 and D2 in the 10–50 cm layers of shrubland II, forested land, and grassland and D1 in the 50–100 cm layers of shrubland II were significantly higher relative to the control. Both D1 and D2 were positively correlated with the contents of soil nutrients and fine particles and were negatively correlated with soil erosion, indicating that D1 and D2 were potential indices for quantifying changes in soil properties and erosion. In conclusion, all types of land-use conversion significantly improved the conditions of the topsoil, but conversion from cropland to shrubland II, forested land, and grassland, especially shrubland II and grassland, were more effective for improving soil conditions in deeper layers. PMID:27527201

  1. Ecosystem service trade-offs and their influencing factors: A case study in the Loess Plateau of China.

    Science.gov (United States)

    Feng, Qiang; Zhao, Wenwu; Fu, Bojie; Ding, Jingyi; Wang, Shuai

    2017-12-31

    Soil erosion control (SEC), carbon sequestration (CAS), and soil moisture (SMO) strongly interact in the semi-arid Loess Plateau. Since SMO has supportive effects on SEC and CAS, it can be considered as ecosystem service (ES), and there is an immediate need to coordinate the relationships among these ecosystem services (ESs) to promote the sustainability of vegetation recovery. In this study, we quantified the ESs, ES trade-offs, and the environmental factors in 151 sample plots in the Ansai watershed, and we used a redundancy analysis (RDA) to clarify the effects of environmental factors on these ESs and their trade-offs. The results were as follows: (1) the general trend in the SEC of vegetation types was Robinia pseudoacacia (CH)>native grass (NG)>small arbor (ST)>Hippophae rhamnoides (SJ)>artificial grass (AG)>Caragana korshinskii (NT)>apple orchard (GY)>crop (CP); the CAS trend was CH>SJ>NT>AG>CP>ST>GY>NG; and the SMO trend was CP>NG>GY>AG>SJ>ST>CH>NT. (2) For SEC-SMO trade-offs, the influence of vegetation type, altitude, silt and sand composition was dominant. The arrangement of NG, AG, and SJ could decrease the extent of the trade-offs. (3) For CAS-SMO trade-offs, vegetation coverage and types were the dominant factors, but the effects were not complex. The extent of these trade-offs was lowest for NT, and that for SJ was the second lowest. (4) Considering the relationships among the three ESs, SJ was the most appropriate afforestation plant. Combing the vegetation types, slope position, slope gradient, and soil properties could regulate these ES relationships. The dominant factors influencing ES trade-offs varied among the different soil layers, so we must consider the corresponding influencing factors to regulate ESs. Moreover, manual management measures were also important for coordinating the ES relationships. Our research provides a better understanding of the mechanisms influencing the relationships among ESs. Copyright © 2017 Elsevier B.V. All

  2. [Soil organic carbon storage changes with land reclamation under vegetation reconstruction on opencast coal mine dump].

    Science.gov (United States)

    Li, Jun-Chao; Dang, Ting-Hui; Guo, Sheng-Li; Xue, Jiang; Tang, Jun

    2014-10-01

    Vegetation reconstruction was an effective solution to reclaim the opencast coal mine dump which was formed in the process of mining. To understand the impact of the vegetation reconstruction patterns' on the mine soil organic carbon (SOC) storage was essential for selecting the methods of vegetation restoration and also important for accurately estimating the potential of the soil carbon sequestration. The study area was on the Heidaigou opencast coal mine, which was 15 years reclaimed coal mine dump in Zhungeer, Inner Mongolia autonomous region, we selected 5 vegetation reconstruction patterns (natural recovery land, grassland, bush land, mixed woodland of arbor and bush, arbor land), and 16 vegetation types, 408 soil samples (0-100 m), to study the effect of the vegetation reconstruction patterns on the SOC storage. The results were showed as follows: (1) on the reclaimed coal mine dump, the vegetation reconstruction patterns significantly affected the SOC content and its distribution in the soil profile (P shrub land > arbor forest > mixed forest of arbor and shrub > natural recovery land, in which the grassland, shrub land and arbor forest were about 2.2, 1.3, and 1.3 times of natural recovery land (2.14 g · kg(-1)) respectively. The total nitrogen (TN) showed the similar trends. (2) Among the vegetation types, Medicago sativa had the highest surface SOC content (5.71 g · kg(-1)) and TN content (0.49 g · kg(-1)), that were 171.3% and 166.7% higher than the natural recovery land, and two times of Hippophae rhamnoides, Amorpha fruticosa + Pinus tabulaeformis and Robinia pseudoacacia. (3) The effect of vegetation types on SOC mainly concentrated in the 0-20 cm depth, and the effect on TN accounted for 40 cm. (4) For the SOC storage, the order was original landform area > reclaimed dump > new dump and grassland > woodland (including arbor and shrub land). After 15 years revegetation, the soil carbon storage of the grassland, shrub land and arbor land were

  3. Effect of storage time and temperature on stability of bioactive compounds in aseptically packed beverages prepared from rose hips and sea buckthorn berries

    National Research Council Canada - National Science Library

    Andersson, Staffan C; Ekholm, Anders; Johansson, Eva; Olsson, Marie E; Sjöholm, Ingegerd; Nyberg, Lena; Nilsson, Annika; Rumpunen, Kimmo

    2015-01-01

    ... concentrate, and wheat germ oil in one of the beverages. Beverages were formulated, pasteurised and filled aseptically in Tetra Brik packages and stored up to 35 days at 4 degrees C or 22 degrees C...

  4. AFLP fingerprinting analysis of some cultivated varieties of sea ...

    Indian Academy of Sciences (India)

    Unknown

    Shaanxi 712100, People's Republic of China. Introduction. The genus Hippophae includes 7 species and 8 subspe- cies in the world (Swenson and Bartish 2002; Bartish et al. 2002; Sun et al. 2002), these species are all diploid of 2n = 24 and are restricted to Qinghai-Xizang plateau and adjacent areas except Hippophae ...

  5. Role of visual and olfactory cues from agricultural hedgerows in the orientation behavior of multicolored Asian lady beetle (Coleoptera: Coccinellidae).

    Science.gov (United States)

    Bahlai, C A; Welsman, J A; Macleod, E C; Schaafsma, A W; Hallett, R H; Sears, M K

    2008-08-01

    Harmonia axyridis Pallas is an introduced lady beetle common in eastern North American agroecosystems. Two-choice behavioral bioassays were performed to determine whether visual and olfactory stimuli from prey and host habitats could elicit taxis in wild-collected H. axyridis adults and whether beetles exhibit a preference among stimuli. Soybean aphid (Aphis glycines Matsumura) spends much of the year in agricultural hedgerows residing on buckthorn (Rhamnus cathartica L), and H. axyridis is frequently observed feeding on aphids in this habitat. Olfactory bioassays were performed in a Y-tube olfactometer and tested the response of beetles to the odor of buckthorn leaves, apple leaves (Malus domestica Borkh.), and buckthorn leaves both naturally and artificially infested with A. glycines. No differences were observed between the numbers of beetles moving toward the odor of buckthorn artificially infested with A. glycines and uninfested buckthorn, but more beetles preferred naturally infested buckthorn over uninfested buckthorn. Visual bioassays were performed in an acrylic tube arena,and tested beetle response to silhouettes and to apple and buckhorn leaves. Beetles were significantly more likely to choose silhouettes over blank space in visual trials. Significantly more beetles moved toward buckthorn leaves than blank space, but beetles did not discern between apple and buckthorn until olfactory cues were also included. This study lays the foundation for future work examining the response of H. axyridis to visual and olfactory cues in Ontario agroecosystems, which could help enhance effectiveness of H. axyridis as a biological control and mitigate its impacts as a pest species.

  6. Site Investigation Report Fort Devens Groups 3,5, and 6. Volume 1 of 2: Report Text

    Science.gov (United States)

    1996-01-01

    Acer rubrum), red-osier dogwood (Comus stolonifera), buckthorn (Rhamnus cathartica), and speckled alder (Alnus rugosa ). Other species observed in the...area include gray birch (Betula populifolia), quaking aspen (Populus tremuloides), white pine (Pinus strobus), white oak ( Quercus alba), northern...black cherry (Prunus serotina), white oak ( Quercus alba), European buckthorn (Rhamnus frangula), and white birch (Betula papyrifera). The open understory

  7. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    purple willow (Salix purpurea). Only the 25% of the interventions was accomplished by the use of secondary plant species, as tamarisk (Tamarix spp.,) blackthorn (Prunus spinosa) , whitethorn (Crataegus spp.), sea-buckthorn (Hipphopae rhamnoides), wild pear (Pyrus pyraster), cottonwood (Populus nigra), eglantine (Rosa spp.), goat-willow (Salix caprea) and cornel (Cornus sanguinea). Better results were achieved with Spanish Broom, a very rural plant that can effectively colonise even poor soils like badlands; as a matter of fact, more than the 75% of the interventions had positive outcomes The efficacy of the consolidation work by the presence of living structures point out an increase of the stability of those interventions older than 4 years, with taking root species present from 54% to 78%. So far, the construction and the reliability of the works have been monitored, in order to capture critical aspects for the success of works and to build a geo-referenced data base of the existing works and their status.

  8. Interim Regional Supplement to the Corps of Engineers Wetland Delineation Manual: Midwest Region

    Science.gov (United States)

    2008-09-01

    production of crops and livestock. Elevation ranges from approximately 100 to 2,000 ft (30 to 600 m) above sea level. Except in Oklahoma and...European buckthorns (Rhamnus frangula, R. cathartica). Bogs are wetlands formed in depressions, such as kettle holes, where precipitation is the...strobus), jack pine (Pinus banksiana), common buckthorn (Rhamnus cathartica), and osage orange (Maclura pomifera) (in floodplains). The following

  9. Environmental Assessment Stockbridge Research Facility

    Science.gov (United States)

    2012-07-01

    SRF has little elevation change with the high point at approximately 1290 feet above sea level and lowest point at approximately 1250 feet. This...open area that is vegetated with pioneer species such as goldenrod, multi-flora rose, hawthorns and buckthorn . The middle portion of the site includes...driveway system. This area is primarily vegetated by pioneer species such as pin cherry, red maple, buckthorn , hawthorn, multi-flora rose, raspberries

  10. Drug: D06734 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available buckthorn family) Jujube seed Major component: Zizybeoside [CPD:C17564 C17565] Therapeutic category of drugs... in Japan [BR:br08301] 5 Crude drugs and Chinese medicine formulations 51 Crude drugs 510 Crude drugs 5100 Crude drugs...08304] Crude Drugs Drugs for Qi Sedative drugs D06734 Jujube seed Crude drugs [BR:br08305] Dicot plants: rosids Rhamnaceae (buckthorn family) D06734 Jujube seed PubChem: 47208385 ...

  11. Variable Isotopic Compositions of Host Plant Populations Preclude Assessment of Aphid Overwintering Sites

    Directory of Open Access Journals (Sweden)

    Michael S. Crossley

    2017-12-01

    Full Text Available Soybean aphid (Aphis glycines Matsumura is a pest of soybean in the northern Midwest whose migratory patterns have been difficult to quantify. Improved knowledge of soybean aphid overwintering sites could facilitate the development of control efforts with exponential impacts on aphid densities on a regional scale. In this preliminary study, we explored the utility of variation in stable isotopes of carbon and nitrogen to distinguish soybean aphid overwintering origins. We compared variation in bulk 13C and 15N content in buckthorn (Rhamnus cathartica L. and soybean aphids in Wisconsin, among known overwintering locations in the northern Midwest. Specifically, we looked for associations between buckthorn and environmental variables that could aid in identifying overwintering habitats. We detected significant evidence of correlation between the bulk 13C and 15N signals of soybean aphids and buckthorn, despite high variability in stable isotope composition within and among buckthorn plants. Further, the 15N signal in buckthorn varied predictably with soil composition. However, lack of sufficient differentiation of geographic areas along axes of isotopic and environmental variation appears to preclude the use of carbon and nitrogen isotopic signals as effective predictors of likely aphid overwintering sites. These preliminary data suggest the need for future work that can further account for variability in 13C and 15N within/among buckthorn plants, and that explores the utility of other stable isotopes in assessing likely aphid overwintering sites.

  12. Res Sep 2014 Cover Tp 08.09.14.cdr

    Indian Academy of Sciences (India)

    Admin

    Breynia vitis-idaea (Burm.f.) Fischer (syn: Breynia rhamnoides Mll.Arg.) of the family. Phyllanthaceae is a large shrub or a small tree of the Indo-Malayan origin. Bark is greyish-brown to light grey in colour. Branches are reddish-brown, often in zig-zag arrangement. Leaves are alternate, smooth, elliptic with an acute tip, ...

  13. Management of cognitive determinants in senile dementia of Alzheimer's type: therapeutic potential of a novel polyherbal drug product.

    Science.gov (United States)

    Sadhu, Ananya; Upadhyay, Prabhat; Agrawal, Aruna; Ilango, Kaliappan; Karmakar, Dipankar; Singh, Gur Prit Inder; Dubey, Govind Prasad

    2014-12-01

    The enigmatic etiology of neurodegenerative diseases poses a challenge for the development of novel and efficient drugs. The objective of the present study was to evaluate the efficacy of a polyherbal (test) formulation on cognitive functions, inflammatory markers and oxidative stress in healthy elderly as well as senile dementia of Alzheimer's type (SDAT) patients. A randomized double-blind placebo- and active-controlled clinical trial was performed in healthy elderly subjects and SDAT patients with an age range of 60-75 years. The polyherbal test formulation along with a placebo was given to healthy elderly subjects while the SDAT patients received either the test formulation containing extracts of Bacopa monnieri (whole plant), Hippophae rhamnoides (leaves and fruits) and Dioscorea bulbifera (bulbils) at a dose of 500 mg or donepezil drug (Aricept) at a dose of 10 mg, twice daily, for a period of 12 months. After every three months, cognitive functions were assessed by determining the mini mental state examination (MMSE) score, digital symbol substitution (DSS; subtest of the Wechsler Adult Intelligence Scale-Revised), immediate and delayed word recall (digital memory apparatus-Medicaid systems, Chandigarh, India), attention span (Attention Span Apparatus-Medicaid systems, Chandigarh, India), functional activity questionnaire (FAQ) and depression (geriatric depression scale) scores. Further inflammatory markers and level of oxidative stress were analyzed using standard biochemical tests. The trial was performed in 109 healthy subjects and 123 SDAT patients of whom 97 healthy subjects and 104 SDAT patients completed the study. Administration of the test formulation for a period of 12 months was effective in improving cognitive functions in the SDAT patients, when compared to the donepezil-treated group, as determined by the DSS (38.984 ± 3.016 vs 35.852 ± 4.906, P = 0.0001), word recall immediate (3.594 ± 1.003 vs 2.794 ± 0.593, P < 0.0001) and attention span

  14. Download this PDF file

    African Journals Online (AJOL)

    Deepak Dhyani

    Effect of auxin treatments on male and female cuttings ... nutraceutical and pharmaceutical companies are enga- ged in the ... out under natural environmental conditions at an altitude of 2600 m .... IBA 50 mg L-1 produced the .... composition of Hippophae and its quality assessment in Gansu ... Changes in sugar, organic.

  15. Efficacy of Hiporhamin (Erebra in the Treatment and Prevention of Viral Diseases in Children

    Directory of Open Access Journals (Sweden)

    I.B. Yershova

    2014-11-01

    Full Text Available The article presents the results of both experimental and clinical studies of Erebra, active substance of which is hiporhamin — dry purified extract from the leaves of sea buckthorn. The effectiveness of the drug has been shown in children with various viral infections.

  16. Management of traumatic tympanic membrane perforation: a comparative study

    Directory of Open Access Journals (Sweden)

    Gao TX

    2017-07-01

    Full Text Available Tianxi Gao,1 Xiaoli Li,2 Juan Hu,1 Weijun Ma,1 Jingjing Li,1 Na Shao,1 Zhenghui Wang1 1Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Department of Dermatology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China Abstract: This prospective study was conducted to evaluate the efficacy of sea buckthorn oil patches in treating traumatic tympanic membrane (TM perforations. We enrolled 370 patients with traumatic TM perforations of different sizes. These patients were randomly assigned to control group and treatment group. In the treatment group, a sterile cotton patch with sea buckthorn oil was used to cover the TM perforations. In the control group, patients were treated with a sterile cotton patch. The healing rate and time were compared between the two groups. We found that the overall healing rate was significantly higher in the treatment group than in the control group. For middle and large TM perforations, sea buckthorn oil treatment led to a significant increase in the healing rate. At 2 months after injury, the duration of healing was, generally, shorter in the treatment group than in the control group (P<0.05. In conclusion, sea buckthorn oil patches are effective in treating middle and large TM perforations, which results in increased healing rates and decreased healing time. Keywords: sea buckthorn oil, tympanic membrane, perforation, regeneration

  17. [The effect of plant oils on the female reproductive system].

    Science.gov (United States)

    Litvinova, L B; Fedorchenko, T V

    1994-01-01

    The influence of a single dose of different plant oils on the rat estrous cycle and fertility was studied. The ovary function activity was ascertained to be inhibited by soybean, peachy or buckthorn oil injection which resulted in the prolongation of the estrous cycle and its phase destruction. The administration of oil provoked temporal fecundation retain (corn, soybean, sunflower-seed or peachy oils) and decreased the number of pregnant animals (peachy, olive or sunflower-seed oils). The number of the ovary corpus luteum, placentas and living fetus in the uterus were declined, and embryonic death was increased in females after peachy, corn, sunflower-seed, soybean, castor or buckthorn oil feeding. It is concluded that vegetable oils depress ovarian folliculogenesis and have not only anti-implantative, but abortive effects to a greater or lesser extent.

  18. The effect of partial substitution of pork back fat with vegetable oils and walnuts on chemical composition, texture profile and sensorial properties of meatloaf

    Directory of Open Access Journals (Sweden)

    Gabriel Dănuţ MOCANU

    2015-08-01

    Full Text Available The present study investigates the effects of the partial substitution of the pork back fat with different vegetable oils (sea buckthorn, walnut and sunflower and walnuts on the chemical composition, texture profile and sensory characteristics of meatloaves. The dry matter and ash content of meatloaf with vegetable oils and walnuts were higher than the control sample (P < 0.05. The cooking loss, energy values and lipid oxidation for the samples with walnuts and vegetable oils were lower than the control sample. The meatloaf sample containing walnuts and sea buckthorn oil had the highest total antioxidant capacity. The partial substitution of pork back fat showed a positive effect on textural and sensorial characteristics. Results reveal that the incorporation of vegetable oils and walnuts has successfully reduced the animal fat content in the finite products while improving the quality characteristics.

  19. Final Environmental Assessment for the Deactivation/Facility Disposition of Atlas Space Launch Complex (SLC-36) at Cape Canaveral Air Force Station, Florida

    Science.gov (United States)

    2005-08-01

    canopy, which is constantly pruned and shaped by windborne salt spray. Coastal strand forms a dense thicket of shrubs, usually dominated by live...disturbed or open areas. Some areas of strand are densely blanketed with catbrier (Smilax auriculata) or coin vine (Dalbergia ecastophyllum). Two State...Station #4. Coastal oak scrub consists of dense, salt- pruned thickets of live oak, sand live oak, myrtle oak, and buckthorn, sometimes densely

  20. The Black Hull Fleet: Multi-Function Assets for Multi-Mission Duty

    Science.gov (United States)

    2011-01-01

    law enforcement and other safety-at- sea missions, Red hull—icebreaking. “Primary” being the operative word: As Coast Guard missions have expanded and...complicated by weather, ocean currents, and competing operational demands. For example, initial operations employed a towed stor- age vessel or “ sea ...Bluebell (WLI 313) COMMISSIONED: 1963 Buckthorn (WLI 642) 65-foot Inland Buoy Tenders COMMISSIONED: 1954 Bayberry (WLI 65400) Elderberry (WLI 65401

  1. Environ: E00128 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00128 Jujube (JP16) Crude drug Oleanolic acid [CPD:C17148], Betulinic acid [CPD:C0...us saponin I-III), Maslinic acid, Alphitolic acid [CPD:C16912], Jujuboside A [CPD:C17831], Jujuboside B [CPD...aringenin, Scopoletin [CPD:C01752], Rutin [CPD:C05625] Ziziphus jujuba [TAX:32696...8] Same as: D06758 Rhamnaceae (buckthorn family) Jujube fruit Major component: Zizyphus saponin ...

  2. Leopold’s Arboretum Needs Upstream Water Treatment to Restore Wetlands Downstream

    OpenAIRE

    Zedler, Joy B.; Doherty, James M.; Isabel M. Rojas

    2014-01-01

    A case study has broad relevance for urban natural reserves. Aldo Leopold’s far-reaching vision to restore historical ecosystems at the UW-Madison Arboretum has been difficult to achieve despite ~80 years of restoration work. Wetlands (~1/4 of the 485-ha reserve) resist restoration, given urban watersheds and inflows of low quality water. Current conditions favor aggressive invasive plants (cattails, reed canary grass, and buckthorn)—species that do not fulfill the 1934 vision. Today, urban ...

  3. Urban trees and forests of the Chicago region

    Science.gov (United States)

    David J. Nowak; Robert E. III Hoehn; Allison R. Bodine; Daniel E. Crane; John F. Dwyer; Veta Bonnewell; Gary. Watson

    2013-01-01

    An analysis of trees in the Chicago region of Illinois reveals that this area has about 157,142,000 trees with tree and shrub canopy that covers 21.0 percent of the region. The most common tree species are European buckthorn, green ash, boxelder, black cherry, and American elm. Trees in the Chicago region currently store about 16.9 million tons of carbon (61.9 million...

  4. Modeling distribution and abundance of soybean aphid in soybean fields using measurements from the surrounding landscape.

    Science.gov (United States)

    Bahlai, C A; Sikkema, S; Hallett, R H; Newman, J; Schaafsma, A W

    2010-02-01

    Soybean aphid (Aphis glycines Matsumura) is a severe pest of soybean in central North America. Outbreaks of the aphid in Ontario are often spotty in distribution, with some geographical areas affected severely and others with few or no aphid populations occurring in soybean for the duration of the season. A. glycines spend summers on soybean and overwinter on buckthorn, a shrub that is widespread in southern Ontario and is commonly found in agricultural hedgerows and at the margins of woodlots. A. glycines likely use both short distance migratory flights from buckthorn and longer distance dispersal flights in the search for acceptable summer hosts. This study aims to model colonization of soybean fields by A. glycines engaged in early-season migration from overwintering hosts. Akaike's information criterion (AIC) was used to rank numerous competing linear and probit models using field parameters to predict aphid presence, colonization, and density. The variable that best modeled aphid density in soybean fields in the early season was the ratio of buckthorn density to field area, although dramatic differences in relationships between the parameters were observed between study years. This study has important applications in predicting areas that are at elevated risk of developing economically damaging populations of soybean aphid and which may act as sources for further infestation.

  5. Charcoal and fossil wood from palaeosols, sediments and artificial structures indicating Late Holocene woodland decline in southern Tibet (China)

    Science.gov (United States)

    Kaiser, Knut; Opgenoorth, Lars; Schoch, Werner H.; Miehe, Georg

    2009-07-01

    Charcoal and fossil wood taken from palaeosols, sediments and artificial structures were analysed in order to evaluate the regional pedoanthracological potential and to obtain information on Holocene environmental changes, particularly on possible past tree occurrences in southern Tibet. This research was initiated by the question to what extent this area is influenced by past human impact. Even recent evaluations have perceived the present treeless desertic environment of southern Tibet as natural, and the previous Holocene palaeoenvironmental changes detected were predominantly interpreted to be climate-determined. The material analysed - comprising a total of 53 botanical spectra and 55 radiocarbon datings from 46 sampling sites (c. 3500-4700 m a.s.l.) - represents the largest systematically obtained data set of charcoal available from Tibet so far. 27 taxa were determined comprising trees, (dwarf-) shrubs and herbs as well as grasses. The predominant tree taxa were Juniperus, Hippophae, Salix and Betula. According to their present-day occurrence in the region, the genera Juniperus and Hippophae can be explicitly attributed to tree species. Further, less frequently detected tree taxa were Populus, Pinus, Quercus, Taxus and Pseudotsuga. Charcoal of Juniperus mainly occurred on southern exposures, whereas Betula was associated with northern exposures. In contrast, the (partly) phreatophytic taxa Hippophae and Salix showed no prevalent orientation. The distribution of radiocarbon ages on charcoal revealed a discontinuous record of burning events cumulating in the Late Holocene (c. 5700-0 cal BP). For southern Tibet, these results indicated a Late Holocene vegetation change from woodlands to the present desertic pastures. As agrarian economies in southern and south-eastern Tibet date back to c. 3700 and 5700 cal BP, respectively, and the present-day climate is suitable for tree growth up to c. 4600 m a.s.l., we concluded that the Late Holocene loss or thinning out

  6. [Comparison of soil fertility among open-pit mine reclaimed lands in Antaibao regenerated with different vegetation types].

    Science.gov (United States)

    Wang, Xiang; Li, Jin-chuan; Yue, Jian-ying; Zhou, Xiao-mei; Guo, Chun-yan; Lu, Ning; Wang, Yu-hong; Yang, Sheng-quan

    2013-09-01

    Re-vegetation is mainly applied into regeneration in opencast mine to improve the soil quality. It is very important to choose feasible vegetation types for soil restoration. In this study, three typical forest restoration types were studied at Antaibao mine, namely, Medicago sativa, mixed forests Pinus taebelaefolius-Robinia pseudoacacia-Caragana korshinskii and Elaeagnus angustifolia-Robinia pseudoacacia-Caragana korshinskii-Hipophae rhamnoides, to determine the nutrient contents and enzyme activities in different soil layers. The results showed that re-vegetation markedly increased soil nutrient contents and the enzyme activities during the restoration process. The nutrient content of soil in the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest field was significantly higher than those in other plots. It was found that the soil of the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest had the highest integrated fertility index values. In conclusion, the restoration effects of the P. zaebelaefolius-R. pseudoacacia-C. Korshinskii mixed forest was better than that of E. angustifolia-R. pseudoacacia-C. korshinskii-H. rhamnoides, while M. sativa grassland had the least effect.

  7. USE OF DIETARY CAMELINA (CAMELINA SATIVA SEEDS DURING THE FINISHING PERIOD; EFFECTS ON BROILER PERFORMANCE AND ON THE ORGANOLEPTIC TRAITS OF BROILER MEAT

    Directory of Open Access Journals (Sweden)

    GEORGETA CIURESCU

    2007-05-01

    Full Text Available The study quantified the effects of Camelina seeds and buckthorn meal on broiler carcass production and quality. Camelina seeds and buckthorn meal were incorporated in the compound feeds for finishing broilers as natural source of vitamins (beta- carotene, vitamins C, B1, B2, E, F, calcium, phosphorus, magnesium, potassium, sodium, and iron. The experiment was conducted on 600 Hybro PN broilers during the age period 7 – 42 days. The broilers were assigned to 3 groups, a control group © and two experimental groups (E1 and E2, each with 200 broilers (3 groups  3 replicates  100 broilers. Three (phase-feeding compound feeds formulations were used. The control diet consisted mainly of corn, full fat soybean, soybean meal and corn gluten. Camelina seeds (10% replaced full fat soybean in the experimental groups, while additionally, in E2 the classical premix with synthetic vitamins and minerals was replaced by buckthorn meal. The partial results show that the use of Camelina seeds reduced significantly (P<0.05 the final live weight, but the liveability percentage was not influenced by the type of dietary compound feed throughout the experimental period. Carcass fat decreased by 61.44% and 30.72% in the experimental groups compared to the control group. Total proteins increased in average by 3-10% concomitantly with the increase of the water content, fat decreased in average by 1.5-4%, the energy value also decreased proportionally with the fat in the experimental groups compared to the control group. Water retention capacity increased by 1% in E2.

  8. Endorsing the declining indigenous ethnobotanical knowledge system of seabuckthorn in Central Himalaya, India.

    Science.gov (United States)

    Dhyani, D; Maikhuri, R K; Misra, S; Rao, K S

    2010-02-03

    Based on research findings this study is aimed to generate database on ethnobotanical aspects, sustainable utilization by value addition and awareness generation through outreach programme related to Hippophae salicifolia D. Don. (Elaeagnaceae) in the higher Himalayan zone of Uttarakhand in Central Himalaya, India. An in-depth survey from June 2004 to July 2006 followed by 480 interviews with the help of semi-structured questionnaires was carried out in 24 Hippophae growing locations in 12 different valleys of Uttarakhand. Plant has immense multipurpose properties and is traditionally utilized for food (20(Min)-90%(Max)), medicine (10(Min)-60%(Max)), veterinary (20(Min)-100%(Max)), fuel (10(Min)-80%(Max)), fencing (20(Min)-80%(Max)), agricultural tools (20(Min)-50%(Max)) and dye mordant (60%). Besides, awareness programmes and value added product demonstration resulted in economical upliftment of local inhabitants of Central Himalaya. The present manuscript will certainly provide an ethnobotanical statistics' impact on the modern scientific societies regarding conservation, cultivation and popularization of this underutilized wild edible species at mass scale. Simultaneously, these findings have important connotations in light of upcoming organic food and nutraceutical industries in the country. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.

  9. The antioxidant activity of selected Romanian honeys

    Directory of Open Access Journals (Sweden)

    Irina DOBRE

    2010-12-01

    Full Text Available Four types of Apis mellifera honey collected in the eastern region of Romania were screened for their total phenolic content by the Folin-Ciocalteau method, for anti radical power as assessed by DPPHradical scavenging assay, for the FRAP assay-ferric reducing antioxidant power. The antioxidant effect over a substrate sensitive to lipid per oxidation and also the presence of some pigments with antioxidant action in honey-ABS 450 were evaluated. All assays revealed the following order of the obtained values: Lime honey>poly-flower honey>Sea buckthorn honey>Acacia honey.

  10. Conservation and utilization of forest resources in the near and remote future

    Energy Technology Data Exchange (ETDEWEB)

    Logginov, B.I.

    1981-01-01

    One of the directions of complex forest utilization is the creation of combined agricultural and forest enterprises specialized by planting different under-canopy crops such as hazelnut, filbert, high-bush cranberry, and cornelian cherry. Future breeding can add other crops such as quince, sea buckthorn, currant, actinidia, Schizandra, etc. The enterprise can also specialize in growing shade-tolerating herbs under the trees. Growing cultivated grass plants and shrubs under tree stock with the use of fertilizers and, if necessary, irrigation, will benefit forest growth particularly in the areas of developed forest industry in the European part of the USSR where there are about 5 million hectares of forest.

  11. Environ: E00105 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00105 Jujube seed (JP16) Crude drug Spinosin [CPD:C17834], Isospinosin [CPD:C17837...], Zizybeoside I [CPD:C17564], Zizybeoside II [CPD:C17565], Protojujuboside A [CPD:C17828], Protojujuboside B [CPD:C17829], Protojuju...boside B1 [CPD:C17830], Jujuboside A [CPD:C17831], Jujuboside B [CPD:C17832], Jujub...ctone, Apigenin-6-C-beta-D-glucopyranoside, Acetyljujuboside B, Isovitexin-2''-O-beta-D-glucopyranoside Zizyphus juju...ba [TAX:326968] Same as: D06734 Rhamnaceae (buckthorn family) Jujube seed Major component: Zizybeoside [CPD:C17564 C17565] ...

  12. [Comparative study of the clinical observation on traumatic perforation of tympanic membrane].

    Science.gov (United States)

    Xin, Wenjun; Zhang, Xiaotong; Cui, Long; Wei, Miao; Yang, Guang; Lei, Juanjuan

    2015-09-01

    To explore the clinical treatment effects of sea buckthorn oil for in different size traumatic perforation of tympanic membrane in different size. Prospective, randomized study of 199 outpatients with traumatic perforation of tympanic membrane who were enrolled between December 2012 and December 2014 after informed consent. The patients were divided into treatment group (101 cases) and control group (98 cases). According to the size of the perforations, patients in each group were divided into large perforation group, middle perforation groups and small perforation group. The cases in large perforation group, middle perforation groups and small perforation group were 36, 34, 31 in treatment group and 35, 33, 30 in control group. The patients in treatment group were treated with sea buckthorn oil once a week, while the patient in control group were self-healing and checked once a week. All the patients were followed-up in two months. The healing rate of two groups was applied for the evaluation indicator of clinical effect. We compared the healing rate, average healing time and phological change of tympanic membrane of patients at the first and second month. The total healing ratio of patients in treatment group is 62.4% and 79.2% compared with 29.6% and 57.1% in control group at the first and second month (P ratios of middle, large perforation groups in treatment group and control group (P ratios of small perforation group in treatment group and control group (P > 0.05). The average healing time of large, middle and small perforation group at the second month are significantly shorter than the control group. It is better to apply observation method and let it self-healed for small traumatic tympanic membrane perforation according to its higher healing ratio. While, it is better to apply sea buckthorn oil method for middle and large traumatic tympanic membrane perforation according to its lower healing ratios. Sea buckthorn oil treatment is benefitial for increasing

  13. Preliminary Studies Regarding the Production of Jam from Organic Rose Petal

    Directory of Open Access Journals (Sweden)

    Ana Cornelia BUTCARU

    2017-11-01

    The final products, seventeen variants of rose jam, were analyzed and tasted. Sensorial analysis was made by consumers of different ages and gender. Jam appearance, general taste, aroma, and the overall impression were noticed. V4 variant - Brother Cadfael with sea buckthorn was the most appreciated variant. For each of these top variants, target group by gender and age was analyzed. The results showed that the customers’ preferences are influenced by age and gender and the organic rose jam is a highly appreciated product.

  14. “SEABUCKTHORNOLOGY” A POSSIBLE NEW INTERDISCIPLINARY SCIENCE

    Directory of Open Access Journals (Sweden)

    Angel PROOROCU

    2015-04-01

    Full Text Available The purpose of the paper was to present a critical overview on the main opinions and research results on "seabuckthornology" as a new interdisciplinary science, as mentioned on various international conferences and other events hold in different countries where the author took part and expressed his opinions as expert in the field. The current opinion of many experts is that the sea buckthorn is the result of a long hard work in the field of research, practice, landscape architecture, production, soil science, animal and human health. It is an important plant of the 3rd millennium. The only problem many experts are facing is the fact that it is very difficult to put in order all multidisciplinary information from Botany, Geology, Marketing, Medicine, Biochemistry, Agronomy, Management etc. The solution is the elaboration of a statute of the interdisciplinary new science "seabuckthornology" and the creation of a multilingual data base, which should be updated permanently as at any moment a manufacturer having sea buckthorn oil production to find many offers from the entire world, obtaining all parameters and prices in few minutes, the address, fax, e-mail, phone number etc. In the actual world crises, a scientifically aboard of seabuckthorn may be a solution to health and environmental problems.

  15. Phenolic compounds extracted by acidic aqueous ethanol from berries and leaves of different berry plants.

    Science.gov (United States)

    Tian, Ye; Liimatainen, Jaana; Alanne, Aino-Liisa; Lindstedt, Anni; Liu, Pengzhan; Sinkkonen, Jari; Kallio, Heikki; Yang, Baoru

    2017-04-01

    Phenolic compounds of berries and leaves of thirteen various plant species were extracted with aqueous ethanol and analyzed with UPLC-DAD-ESI-MS, HPLC-DAD, and NMR. The total content of phenolics was consistently higher in leaves than in berries (25-7856 vs. 28-711mg/100g fresh weight). Sea buckthorn leaves were richest in phenolic compounds (7856mg/100g f.w.) with ellagitannins as the dominant compound class. Sea buckthorn berries contained mostly isorhamnetin glycosides, whereas quercetin glycosides were typically abundant in most samples investigated. Anthocyanins formed the dominating group of phenolics in most dark-colored berries but phenolic acid derivatives were equally abundant in saskatoon and chokeberry berries. Caffeoylquinic acids constituted 80% of the total phenolic content (1664mg/100g f.w.) in bilberry leaves. B-type procyanidins and caffeoylquinic acids were the major phenolic compounds in hawthorn and rowanberry, respectively. Use of leaves of some species with prunasin, tyramine and β-p-arbutin, may be limited in food applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Mud-plastered granary-baskets at a Celtic Oppidum near Čarnok (Vojvodina, Serbia

    Directory of Open Access Journals (Sweden)

    Medović Aleksandar

    2011-01-01

    Full Text Available In the Celtic Oppidum near Čarnok (Bačka, Vojvodina, Serbia remains of four mud-plastered granarybaskets were discovered. They are all dated to the period Gomolava VI-B (first half of the 1st century B.C.. Hulled barley and broomcorn millet were stored in the granary-baskets. The mesh of the granary-baskets was composed of young branches of oak tree (Quercus, common English elm tree (Ulmus cf. campestris L., poplar / willow (Populus / Salix, purgin buckthorn (Rhamnus cf. cathartica L., wayfaring tree (Viburnum cf. lantana L., spindle tree (Euonymus cf. europaeus L., barberry (Berberis vulgaris L. and a Pomoidae-tree. At least four different tree taxa were used for basket making. Construction of two-thousand-year old mud-plastered granary-baskets from the Pannonian plain is very similar to that of traditional granaries in some present-day villages in Africa. Additionally, one non-plastered basket was also discovered at Oppidum. It was build out of maple (Acer, barberry, buckthorn (Rhamnus and branches of a pomaceous fruits tree (Pomoidae. .

  17. Effect of wood ash and K-fertilization on {sup 137}Cs uptake by selected forest plants

    Energy Technology Data Exchange (ETDEWEB)

    Mandro, Yrii N. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Rosen, Klas [Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden); Vinichuk, Mykhailo M. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden)

    2014-07-01

    Accumulation {sup 137}Cs by different forest plants and trees after fertilization of soil with potassium and wood ash ({sup 137}Cs-contaminated and non-contaminated) in forest ecosystems of Ukraine contaminated by radionuclides after Chernobyl accident in 1986 was studied. Experiment is performed in Bazar forestry, Zhytomyr region, Ukraine, located about 70 km (51 deg. 5'35'' N, 29 deg. 18'56'' E) from Chernobyl NPP. Potassium fertilizer (KCl, wooden ash (Ash), and {sup 137}Cs-contaminated ash ({sup 137}CsAsh) in different combinations) were spread on the forest floor in April 2012 at a rate corresponding 100 kg/ha potassium. The experiment layout was as follows: 1- Control (no fertilizers were applied), 2- KCl, 3- Ash + KCl, 4- Ash + {sup 137}CsAsh, 5- Ash, 6- {sup 137}CsAsh + KCl. Samples (leaves and annual shoots) of blueberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), rowan (Sorbus aucuparia), birch (Betula), buckthorn (Frangula) and oak (Quercus robur) and also mushrooms (fruit bodies of Russula, Lactarius, Cantharellus, Collybia etc.) and berries (blueberry and cowberry) were taken monthly from each treatment. Samples were measured for {sup 137}Cs with calibrated HPGe detectors. The results of the first year studies show variation of Transfer Factors (TF) for different plants and for the same plants on different treatments. The effect of fertilization was found for blueberry on Ash-applicated (TF = 0.0085 ± 0.0025), {sup 137}CsAsh + KCl-applicated (TF = 0.0105 ± 0.0060) and Ash + KCl-applicated (TF = 0.0123 ± 0.0058) treatments compared to Control (TF = 0.0163 ± 0.0092). Also good effect for rowan on Ash + KCl-applicated treatment (TF = 0.0067 ± 0.0024) compared to Control (TF = 0.0100 ± 0.0064). Effect was less obvious for birch on Ash + KCl-applicated treatment and for cowberry on Ash + KCl-applicated treatment. There was not found an obvious effect of fertilization for buckthorn. Positive effect of

  18. Profiling and Quantification of Regioisomeric Caffeoyl Glucoses in Berry Fruits.

    Science.gov (United States)

    Patras, Maria A; Jaiswal, Rakesh; McDougall, Gordon J; Kuhnert, Nikolai

    2018-02-07

    On the basis of a recently developed tandem mass spectrometry-based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected berry fruits were profiled for their caffeoyl glucose ester content. Fresh edible berries profiled, including strawberries, raspberries, blueberries, blackberries, red currant, black currant, lingonberries, gooseberries, and juices of elderberries, goji berries, chokeberries, cranberries, açai berries, sea buckthorn berries, Montmorency sour cherries, and pomegranates, were investigated. 1-Caffeoyl glucose was found to be the predominant isomer in the majority of samples, with further profiling revealing the presence of additional hydroxycinnamoyl glucose esters and O-glycosides with p-coumaroyl, feruloyl, and sinapoyl substituents. A quantitative liquid chromatography-mass spectrometry-based method was developed and validated, and all caffeoyl glucose isomers were quantified for the first time in edible berries.

  19. Preparation, linear and NLO properties of DNA-CTMA-SBE complexes

    Science.gov (United States)

    Manea, Ana-Maria; Rau, Ileana; Kajzar, Francois; Meghea, Aurelia

    2013-10-01

    Synthesis of deoxyribonucleic acid (DNA) - was cetyltrimethylammonium (CTMA) - sea buckthorn extract (SBE) at different concentrations is decribed. The complexes were processed into good optical quality thin films by spin coating on different substrates such as: glass, silica and ITO covered glass substrates. SBE contains many bioactive substances that can be used in the treatment of several diseases, such as cardiovascular disease, cancer, and acute mountain sickness. The obtained thin films were characterized for their spectroscopic, fluorescent, linear and nonlinear optical properties as function of SBE concentration. The third-order nonlinear optical (NLO) properties of thin films were determined by the optical third-harmonic generation technique at 1 064.2 nm fundamental wavelength.

  20. ANATOMIC INVESTIGATION OF HUNGARY'S COMMON SHRUB SPECIES

    Directory of Open Access Journals (Sweden)

    Eszter ANTALFI

    2015-09-01

    Full Text Available In Hungary a huge part of wooden plants are shrubs. Flora of hungarian forests is among the richest in Europe. Many plants can be classified as shrubs or trees as well, circumstances during their development define what they will become. The diverse world of shrubs and weeds delights the eye under 20-30 meter high trees. From these there are some well known which basically everybody recognises is lilac (Syringa vulgaris, elderberry (Sambucus nigra, dog-rose (Rosa canina, single-seeded hawthorn (Crataegus monogyna and common buckthorn (Rhamnus cathartica. To get these species better known – and occasionally foreshadowing their wood industry usage in some way – it is expendient to familiarize ourselves with their microscopic structure and characteristics. Nowadays there are several imaging methods known, however for examining floral tissue the optical microscope is still the most common one to be used.

  1. Toxic Effects of Rhamnus alaternus: A Rare Case Report

    Directory of Open Access Journals (Sweden)

    H. Ben Ghezala

    2015-01-01

    Full Text Available In Tunisia, there are about 478 species of plants commonly used in folk medicine. Medicinal plants and herbal remedies used are responsible for 2% of intoxications listed by Tunisian National Poison Center. Most cases are related to confusion between edible plants and toxic plants lookalikes or to an excessive consumption of therapeutic plants. We report the case of a 58-year-old man admitted to the Emergency Department of the Regional Hospital of Zaghouan (Tunisia, with renal failure and rhabdomyolysis. The patient reported having daily consumption of a homemade tea based on Mediterranean Buckthorn roots, during the last 6 months to treat type 2 diabetes. The aim of this work was to establish an association between the consumption of the herbal remedy and the occurrence of both renal failure and rhabdomyolysis. No similar cases have been reported in recent literature.

  2. Antioxidant activity of medicinal plant extracts in cookies.

    Science.gov (United States)

    Mišan, Aleksandra; Mimica-Dukić, Neda; Sakač, Marijana; Mandić, Anamarija; Sedej, Ivana; Simurina, Olivera; Tumbas, Vesna

    2011-01-01

    The antioxidant activity of ethanolic extracts of parsley, buckthorn, mint, caraway, and their mixture "Vitalplant" was evaluated, and the potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was tested. The antioxidant activity was estimated by 2 direct (ESR) and 4 indirect (spectrophotometric) tests and correlated with the total phenolic and flavonoid content. The potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was measured by thiobarbituric acid (TBA)-reactive-substances assay (TBARS) and DPPH˙ (1,1-diphenyl-2-picryl-hydrazyl) test. Significantly different (P total phenolics were found among extracts in the following order: mint > buckthorn > "Vitalplant" > parsley > caraway. Total flavonoid content varied from 0.510% (parsley) to 2.05% ("Vitalplant"). A statistically significant correlation was found between IC(50) values on DPPH˙ and total flavonoid content of the samples (r=- 0.94, P activity (r= 0.89, P activity and total phenolic content was found to be positive and statistically significant (r= 0.94, P antioxidant activity in most of the tests, which can be explained by synergistic effects of the ingredients of which it is composed. Finally, "Vitalplant" extract addition (2%, 4%, and 6%) improved antioxidant activity and oxidative stability of the cookies in dose-dependent manner. Supplementation of cookies with a mixture of Petroselini fructus, Frangulae cortex, Mentha piperitae folium, Carvi fructus can retard the process of lipid oxidation and elevate antioxidant activity of the final product. © 2011 Institute of Food Technologists®

  3. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage

    Energy Technology Data Exchange (ETDEWEB)

    Istrati, D.; Lacatusu, I.; Bordei, N.; Badea, G.; Oprea, O. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Stefan, L.M. [National Institute of Research and Development for Biological Sciences, Splaiul Independentei Street No. 296, 060031 Bucharest (Romania); Stan, R. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Badea, N., E-mail: nicoleta.badea@gmail.com [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Meghea, A. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania)

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to − 45 mV) and displayed average sizes of 70 nm to 140 nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract–bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. - Highlights: • Safety phyto-mediated nanostructured carriers (NLC) based on two kinds of bioactives • Carrot extract incorporation into nanostructured carriers ranged from 78 to 88.3%. • High antioxidant activity of NLC by scavenging up to 98% free oxygen radicals • Extract entrapment represents a viable way for an equilibrated release of carotenoids. • Remarkable regenerative effect of L929 cell, with a proliferation of 133.4%.

  4. Effect of Plant Antimicrobial Agents Containing Marinades on Storage Stability and Microbiological Quality of Broiler Chicken Cuts Packed with Modified Atmosphere Packaging.

    Science.gov (United States)

    Alakomi, H-L; Maukonen, J; Honkapää, K; Storgårds, E; Quirin, K-W; Yang, B; Saarela, M

    2017-10-01

    The food industry, including the meat industry, is currently looking for natural preservatives to prevent the growth of harmful microbes in foods. The potential of plant-derived antimicrobial extracts to increase the shelf life and to delay the microbiological spoilage of marinated broiler chicken cuts in modified atmosphere packages during cold storage was investigated in this study. We evaluated the impact of aqueous ethanolic extracts of Finnish sea buckthorn berries and lingonberries and supercritical CO2-extracted herbal extracts from an antimicrobial blend and oregano leaves on the shelf life of broiler meat. The commercial antimicrobial blend extract and the oregano extract inhibited the growth of lactic acid bacteria (LAB) and Brochothrix thermosphacta in the marinated samples. The antimicrobial blend extract also reduced the growth of psychrotrophic aerobic bacteria, whereas the sea buckthorn and lingonberry extracts did not. Only minor antimicrobial activity against Enterobacteriaceae by all the extracts was observed. Plate count analysis, denaturing gradient gel electrophoresis, and quantitative real-time PCR indicated that LAB, which are the major spoilage group in marinated modified atmosphere-packaged poultry products, were not significantly affected by the berry extracts studied. During this shelf-life study, LAB isolates of Lactobacillus and Leuconostoc were identified in the marinated samples. Antimicrobial blends and oregano leaf extracts can act as antimicrobial agents in marinade blends, although tailoring of the dose is needed because of their strong taste. Further studies for exploiting synergistic effects of plant extracts could contribute to the development of potential and more effective antimicrobial blends. Studies are needed in meat matrices and in product applications to demonstrate the efficacy of these compounds.

  5. Technogenic Ecological Sequences in Tailing Pond from Căpușu Mare, Built between 1975-1981

    Directory of Open Access Journals (Sweden)

    Aurel MAXIM

    2017-11-01

    Full Text Available In Romania, all actions related to mining management are found in “Strategy of the mining industry for 2012-2035”. Today, numerous tailing ponds have  remained outside rehabilitation operations, and they are becoming more natural, as is the case of the tailing pond in Capusu Mare. The vegetation study was conducted in 2015, at the tailing pond III in Căpușu Mare that was operational between 1975-1981. The following phytopopulation and phytocenotic indices were calculated: presence, frequency, class of presence, abundance-dominance and average abundance-dominance (ADm of species. Floral studies show the presence of 40 plant species. The wood species with the highest mean dominant abundance are Hippophäe rhamnoides (14.78% and Salix alba (10.55%, and of herbaceous species stands Phragmites communis with 7.49%. After 34 years from the heap closure, the degree of vegetation coverage is 77%. Wood species occupy about 32% of the heap surface.

  6. Salt stress-induced changes in antioxidative defense system and proteome profiles of salt-tolerant and sensitive Frankia strains.

    Science.gov (United States)

    Srivastava, Amrita; Singh, Anumeha; Singh, Satya S; Mishra, Arun K

    2017-04-16

    An appreciation of comparative microbial survival is most easily done while evaluating their adaptive strategies during stress. In the present experiment, antioxidative and whole cell proteome variations based on spectrophotometric analysis and SDS-PAGE and 2-dimensional gel electrophoresis have been analysed among salt-tolerant and salt-sensitive Frankia strains. This is the first report of proteomic basis underlying salt tolerance in these newly isolated Frankia strains from Hippophae salicifolia D. Don. Salt-tolerant strain HsIi10 shows higher increment in the contents of superoxide dismutase, catalase and ascorbate peroxidase as compared to salt-sensitive strain HsIi8. Differential 2-DGE profile has revealed differential profiles for salt-tolerant and salt-sensitive strains. Proteomic confirmation of salt tolerance in the strains with inbuilt efficiency of thriving in nitrogen-deficient locales is a definite advantage for these microbes. This would be equally beneficial for improvement of soil nitrogen status. Efficient protein regulation in HsIi10 suggests further exploration for its potential use as biofertilizer in saline soils.

  7. [Species-associated differences in foliage-root coupling soil-reinforcement and anti-erosion].

    Science.gov (United States)

    Liu, Fu-quan; Liu, Jing; Nao, Min; Yao, Xi-jun; Zheng, Yong-gang; Li, You-fang; Su, Yu; Wang, Chen-jia

    2015-02-01

    This paper took four kinds of common soil and water conservation plants of the study area, Caragana microphylla, Salix psammophila, Artemisia sphaerocephala and Hippophae rhamnides at ages of 4 as the research object. Thirteen indicators, i.e., single shrub to reduce wind velocity ration, shelterbelt reducing wind velocity ration, community reducing wind velocity ration, taproot tensile strength, representative root constitutive properties, representative root elasticity modulus, lateral root branch tensile strength, accumulative surface area, root-soil interface sheer strength, interface friction coefficient, accumulative root length, root-soil composite cohesive, root-soil composite equivalent friction angle, reflecting the characteristics of windbreak and roots, were chose to evaluate the differences of foliage-root coupling soil-reinforcement and anti-erosion among four kinds of plants by analytic hierarchy process (AHP) under the condition of spring gale and summer rainstorm, respectively. The results showed the anti-erosion index of foliage-root coupling was in the sequence of S. psammophila (0.841) > C. microphylla (0.454) > A. sphaerocephala (-0.466) > H. rhamnides (-0.829) in spring gale, and C. microphylla (0.841) > S. psammophila (0. 474) > A. sphaerocephala (-0.470) > H. rhamnides (-0.844) in summer rainstorm. S. psammophila could be regarded as one of the most important windbreak and anti-erosion species, while C. microphylla could be the most valuable soil and water conservation plant for the study area.

  8. A study of the development of bio-energy resources and the status of eco-society in China

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xia; Huang, Yongmei; Gong, Jirui [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Zhang, Xinshi [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Institute of Botany, CAS, Beijing 100093 (China)

    2010-11-15

    Industrialization of bio-energy relies on the supply of resources on a large scale. The theoretical biomass resources could reach 2.61-3.51 billion tce (tons of coal equivalent)/a in China, while the available feedstock is about 440-640 million tce/a, however, among this only 1.5-2.5% has been transferred into energy at present. Marginal land utilization has great prospects of supplying bio-energy resources in China, with co-benefits, such as carbon sequestration, water/soil conservation, and wind erosion protection. There is a large area of marginal land in China, especially in northern China, including about 263 million ha of desertification land, 173 million ha of sand-land, and 17 million ha of salinizatin land. The plant species suitable to be grown in marginal lands, including some species in Salix, Hippophae, Tamarix, Caragana, and Prunus is also abundant Biomass feedstock in marginal lands would be 100 million tce/a in 2020, and 200 million tce/a in 2050. As a result, a win-win situation of eco-society and bio-energy development could be realized, with an expected 4-5% reduction of total CO{sub 2} emission in China in 2020-2050. Although much progress has been made in the field of bio-energy research in China, yet significant efforts should be taken in the future to fulfill large-scale industrialization of bio-energy. (author)

  9. TO A QUESTION OF FEASIBILITY OF APPLICATION OF 100 HZ VIBRATION IN TREATMENT AND REHABILITATION OF THE PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

    Directory of Open Access Journals (Sweden)

    T. N. Zaripova

    2016-01-01

    Full Text Available The aim of this research is study of feasibility of application of instrumental 100 Hz vibration of the thorax for rehabilitation of COPD patients.Materials and methods. This aim is achieved by clinical observation of 43 COPD patients of 1st and 2nd severity, 77.4 % from which were patients with low risk of exacerbations. Research has been carried out during the stable period of disease. The following parameters have been analyzed: dynamics of the clinical state, spirometry data, clinical - biochemical indicators of blood reflecting presence of the inflammatory process activity, a number of indicators of immune system. Two groups of patients, which were equivalent in an initial state on age, disease severity, expressiveness of functional violations, have been isolated. The 1st group contained 20 persons was the main one. It received 100 Hz vibration impact on the thorax on the background of exercises with physical therapy and inhalations of physiological solution. The 2nd group contained 23 persons obtained in addition sea-buckthorn paste.Results. It has been revealed that the course of 100 Hz vibration impact on the thorax is accompanied, mainly, with positive subjective dynamics in the form of decrease of frequency and expressiveness of complaints. At that it has been noted improvement of bronchial passability due to improved drainage of bronchial tubes, but only at the patients with initial violations of ventilation. Only additional reception of sea-buckthorn paste on the background of vibrotherapy contributes to decrease of subclinical inflammation activity and strengthening of immunological protection. Essential positive results of treatment have been reached only for a half of the surveyed patients that allows to speak about non-expediency or low expediency of use of 100 Hz vibration for CODT patients even 1st and 2nd severity in the stable period of course of the disease.

  10. Fusarium Head Blight Control and Prevention of Mycotoxin Contamination in Wheat with Botanicals and Tannic Acid

    Directory of Open Access Journals (Sweden)

    Hans-Rudolf Forrer

    2014-02-01

    Full Text Available Suspensions or solutions with 1% of Chinese galls (Galla chinensis, GC or 1% of tannic acid (TA, inhibited germination of conidia or mycelium growth of Fusarium graminearum (FG by 98%–100% or by 75%–80%, respectively, whereas dried bark from buckthorn (Frangula alnus, FA showed no effect at this concentration. In climate chamber experiments where the wheat variety “Apogee” was artificially inoculated with FG and F. crookwellense (FCr and treated with 5% suspensions of TA, GC and FA, the deoxynivalenol (DON content in grains was reduced by 81%, 67% and 33%, respectively. In field experiments with two commercial wheat varieties and artificial or semi-natural inoculations, mean DON reductions of 66% (TA and 58% (FA, respectively, were obtained. Antifungal toxicity can explain the high efficacies of TA and GC but not those of FA. The Fusarium head blight (FHB and mycotoxin reducing effect of FA is probably due to elicitation of resistance in wheat plants. With semi-natural inoculation, a single FA application in the first half of the flowering period performed best. However, we assume that applications of FA at the end of ear emergence and a treatment, triggered by an infection period, with TA or GC during flowering, might perform better than synthetic fungicides.

  11. Acceptance of Nordic snack bars in children aged 8–11 years

    Directory of Open Access Journals (Sweden)

    Anna Holmer

    2012-04-01

    Full Text Available Background: A health promoting diet is suggested to be tailored to regional circumstances to preserve the cultural diversity in eating habits, as well as contribute to more environmentally friendly eating. It may influence consumer acceptance, however, if the components of the diet differs considerably from their habitual food. Objective: This study aimed to investigate whether snack bars composed of Nordic ingredients were accepted by 8–11 year-old Danish (n=134 and Swedish (n=109 children.Design: A seven-point hedonic scale was used to measure the children's acceptance of five snack bars that varied in their composition of whole grains, berries and nuts. A preference rank ordering of the five bars was also performed. Results: The results showed that samples that were rated highest in liking and were most preferred in both countries were a kamut/pumpkin bar and an oat/cranberry bar. The sample with the lowest rating that was also least preferred was a pumpernickel/sea buckthorn bar. Flavour was the most important determinant of overall liking followed by texture, odour and appearance. Conclusions: Children's acceptances and preferences were highly influenced by the sensory characteristics of the bars, mainly flavour. In agreement with earlier studies, the novel food ingredients seemed to influence children's preferences. The Nordic snack bars may have a potential to be a snack option for Danish and Swedish school children, but repeated exposures to the products are recommended to increase children's acceptance.

  12. Evaluation of Beeswax Influence on Physical Properties of Lipstick Using Instrumental and Sensory Methods

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    Giedre Kasparaviciene

    2016-01-01

    Full Text Available The aim of this study was to optimize the lipsticks formulation according to the physical properties and sensory attributes and investigate the relationship between instrumental and sensory analyses and evaluate the influence of the main ingredients, beeswax and oil, with analysis of lipsticks properties. Central composite design was used to optimize the mixture of oils and beeswax and cocoa butter for formulation of lipsticks. Antioxidant activity was evaluated by DPPH free radical scavenging method spectrophotometrically. Physical properties of lipsticks melting point were determined in a glass tube; the hardness was investigated with texture analyzer. Sensory analysis was performed with untrained volunteers. The optimized mixture of sea buckthorn oil and grapeseed oil mixture ratio 13.96 : 6.18 showed the highest antioxidative activity (70±0.84% and was chosen for lipstick formulation. According to the sensory and instrumental analysis results, optimal ingredients amounts for the lipstick were calculated: 57.67% mixture of oils, 19.58% beeswax, and 22.75% cocoa butter. Experimentally designed and optimized lipstick formulation had good physical properties and high scored sensory evaluation. Correlation analysis showed a significant relationship between sensory and instrumental evaluations.

  13. Effect of Various Phyto-extracts on Physico-chemical, Colour, and Oxidative Stability of Pork Frankfurters

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    Rajesh V. Wagh

    2015-08-01

    Full Text Available Lipid oxidation, colour stability and physico-chemical quality of pork frankfurters with the incorporation of 0.30% sea buckthorn (SBT, 0.10% grape seed (GSE, 0.03% green tea (GTE, 0.12% fenugreek seed (FSE and 0.10% Acacia catechu (ACE were studied during 20 days of refrigerated aerobic storage. The SBT and ACE were identified as being the most effective antioxidants to retard lipid oxidation with the potency decreasing in the following order: SBT>ACE>GSE>GTE>FSE based on thiobarbituric acid reacting substances, peroxide value and free fatty acids. In all samples pH and aw decreased during storage period. The L* value of treated as well as control samples decreased over time while SBT and ACE exhibited an increased redness producing higher a* values than other treatments. However, GTE was more effective in increasing b* values than other treatments at the end of storage. The results suggest that functional plant-derived extracts can be valuable to the modification of frankfurter formulations for improved oxidative stability as well as quality characteristics.

  14. Proposition de classement des sources végétales d’acides gras en fonction de leur profil nutritionnel

    Directory of Open Access Journals (Sweden)

    Dubois Virginie

    2008-01-01

    Full Text Available The role of isolated fatty acids on human health has long been studied by the nutritionists. The consequence of this research for the food industry was a need for a better control of the lipidic profile of manufactured food products. The purpose of the present paper was to give the nutritional profile of 53 vegetable oils in relation with their fatty acid composition and their related nutritional potential. In a first section, the role of isolated fatty acids on blood lipids (LDL-Cholesterol, HDL-Cholesterol and triacylglycerols has been reviewed. In a second section, the vegetable oils are gathered in relation with their similar fatty acids composition, with the aim of classifying their properties in accordance with their role on blood lipids. From a nutritional point of view, besides the major α-linolenic acid source that is rapeseed oil, the classification makes some interesting sources to stand out. They contain either mainly α-linolenic acid (camelin, linseed and perilla or both essential fatty acids as α-linolenic and linoleic acids (purslane, chia, salicorna and sea buckthorn seeds. Moreover, this collection is an interesting tool for new designed food products with a better lipid composition.

  15. Determination of the floral origin of some Romanian honeys on the basis of physical and biochemical properties

    Science.gov (United States)

    Cimpoiu, Claudia; Hosu, Anamaria; Miclaus, Vasile; Puscas, Anitta

    The aim of this study was to determine the physical and biochemical properties of some Romanian honeys in order to discriminate between their floral origins. The evaluated properties were total phenolic content, total protein content, total free amino acids content, color intensity (ABS450), pH, ash content, antioxidant activity. Twenty-six commercial honeys from six types of flowers (acacia, sunflower, forest, polyfloral, lime and Sea Buckthorn) were investigated. All samples showed considerable variations with reference to their properties. The properties values were in the range of approved limits (according to EU legislation). The total phenolic, total protein and total free amino acids contents and color intensity varied considerably. Similarly, forest honey had the highest antioxidant activity while the lowest was found in acacia honey. Correlation between the floral origin of honeys and the physical and biochemical properties, respectively, was observed. Moreover, this study demonstrates remarkable variation in DPPH scavenging activity and content of total phenols in honey, depending on its botanic source.

  16. Cold hardiness research on agricultural and horticultural crops in Finland

    Directory of Open Access Journals (Sweden)

    L. LINDÉN

    2008-12-01

    Full Text Available This paper represents an overview of cold hardiness research conducted on agricultural and horticultural crops, as well as on amenity plants in Finland. Inadequate freezing tolerance and/or winter hardiness often prevents introduction of new species and cultivars to Finland. Field observations on winter hardiness and more recently the results from laboratory freezing tests, have assisted breeders to select hardy genotypes. Research approaches for agricultural crops have evolved from observations on winter and frost damage to studies on molecular mechanisms of cold acclimation and freezing injury. The results of experiments on survival of winter cereals, grasses and clovers and frost tolerance of potato and turnip rape are discussed. The studies conducted on horticultural crops, including apple, strawberry, raspberry, currants, blueberry, sea buckthorn, perennial herbs as well as on ornamental trees and shrubs have included field evaluations of cultivars, or selections for winter hardiness, and studies on the effects of cultural management practices on winter survival. During the last decade detailed studies including controlled freezing tests have provided tools to assist in explanation of the underlying mechanisms of cold hardiness also in horticultural plants. ;

  17. [The major sources of daily dietary intake of micronutrients in the adult rural population of the Republic of Chuvashia].

    Science.gov (United States)

    Khokhlova, E A

    2007-01-01

    The paper presents the results of determination of the basic sources of micronutrients in the nutrition of the rural population of the Chuvash Republic. The importance of foodstuffs, the sources of nutrients, was determined by factor mathematical analysis, by estimating the weight factors. All the products used in the daily ration were studied, which permitted elucidation of the importance of individual Russia-specific products. By reason of the uniform pattern of nutrition in Chuvashia, virtually all study micronutrients were found to come with baked goods, milk, and potatoes. The quantity of fresh vegetables and fruits in the actual diets of the examinees is so low that it exerts no significant effect on the intake of vitamins and minerals. In addition, all vegetables and fruits used in the daily ration are generally used in the personal subsidiary plots. Berries that commonly grow in Chuvashia, such as cranberry, bilberry, sea-buckthorn, and red whortleberry, were also observed to be virtually unused in nutrition, although it is just the berries that are gold mines of vitamins and minerals.

  18. Status of bioactive compounds in foods, with focus on fruits and vegetables.

    Science.gov (United States)

    Shashirekha, M N; Mallikarjuna, S E; Rajarathnam, S

    2015-01-01

    Components of cereals, legumes, pulses, proteins, sea food, milk, carbohydrates and lipids are being evaluated for their influence on human health, as biofunctional compounds. However, references dealing with fruits and vegetables exceed any other food group and accordingly their focus. Fruits and vegetables abound in a spectacular range of such health influencing compounds and thus, study of their bioactivity, in lieu of their consumption in fresh or processed form. Anti-cancerous phenolics from Phyllanthus, radioprotective Litchi phenolics/flavonoids, hypoglycemic Sygium, quercitin and hydroxyl cinnamates of Sweet cherries, xanthones of Mangosteen, ellagitannins of Pomegranate, ursolic acid of Sea buckthorn, muscle relaxative watermelon, anti-cholesterolemic soluble fibre and sterols, cardioprotective saponins, ACE-inhibitory potato hydrolysates, anti-pancreatic cancerous ascorbic acid, carotenoids including pro-vitamin A are few examples unraveled. Thus, the imminent scope to obviate their structural chemistry, influence on storage and processing conditions, factors favoring their bio-accessibility/bio-availability in the food formulations, influencing human health. It is the meticulous combination of these compounds in daily consumption that determines their usefulness to human body. What is of paramount importance is the actual health benefits accrued from consumption of such functional- compound based fresh/processed fruits,vegetables or other foods.

  19. Avoidance of nonhost plants by a bark beetle, Pityogenes bidentatus, in a forest of odors

    Science.gov (United States)

    Byers, John A.; Zhang, Qing-He; Birgersson, Göran

    The bark beetle, Pityogenes bidentatus (Coleoptera: Scolytidae), searches in mixed conifer and deciduous forests of northern Europe for suitable branches of its host, Scots pine (Pinus sylvestris). We tested whether odors from several diverse nonhost trees and plants common in the habitat (e.g., mountain ash, Sorbus aucuparia; oak, Quercus robur; alder buckthorn, Frangula alnus; blueberry, Vaccinium myrtillus; raspberry, Rubus idaeus; and grass, Deschampsia flexuosa) would reduce the attraction of the bark beetle to traps releasing its aggregation pheromone components in the field. Volatiles from the leaves or bark of each of these plants significantly reduced the attraction of the beetles to their pheromone. Odors collected from these nonhosts and analyzed by GC/MS contained monoterpenes, sesquiterpenes, and ``green-leaf'' alcohols, several of which (e.g., 1-octene-3-ol and β-caryophyllene) reduced the attraction to pheromone in the field and elicited electroantennographic responses. In the laboratory, reproduction by the beetle was marginal in nonhost Norway spruce, Picea abies, and was absent in the other nonhost trees. Olfactory avoidance of unsuitable nonhosts may have evolved due to advantages in avoiding mistakes during host selection.

  20. Addendum to aphids’ fauna, feeding on flowering plants in Krasnoyarsk krai

    Directory of Open Access Journals (Sweden)

    D. L. Grodnitsky

    2016-06-01

    Full Text Available The paper reports on new and previously known data on insufficiently studied fauna of aphids living on flowering plants in Krasnoyarsk region, the main part of a vast geographical province named Central or Middle Siberia, depending on terminology used by authors of literature sources. From physical geography viewpoint, the surveyed territory belongs to the Yenisey river basin. Aphids are an ecologically and morphologically diverse group of insects included in 11 families of the suborder Aphidinea in insect fauna of Russian Federation and 25 families in the world’s fauna. Geographical location, dates of collection of aphid colonies are presented with identification of species-specific feeding preferences. First found in Siberia were Symydobius nanae, Euceraphis betulae, Cavariella cicutae, C. konoi, Uroleucon (Dactynotus aeneum, U. giganteum, U. nigrocampalulae, U. trachelii, Aphis agrimoniae, A. coronillae, A. mongolica, A. neothalictri, A. pruni, A. pseudocomosa, A. pulsatillae, A. sambuci, A. (Debilisiphon umbelliferarum. Collected for the first time from willow were Betacallis comes (normally feeds on birch and alder, B. quadrituberculata (common on birch, colonies of Metopeurum fuscoviride were first found on Achillea millefolium. Aphis craccivora was previously collected from plants of different families, but was never seen on Cruciferae (Capsella bursa­pastoris. Aphis frangulae was concidered to feed exclusively on buckthorn, however, it was found on Chamaenerion angustifolium. Judging on diversity of host species, some aphids may be more important for agriculture than it was previously thought. A general review of Krasnoyarsk Krai aphid fauna is suggested for the first time ever.

  1. Evaluation of Beeswax Influence on Physical Properties of Lipstick Using Instrumental and Sensory Methods.

    Science.gov (United States)

    Kasparaviciene, Giedre; Savickas, Arunas; Kalveniene, Zenona; Velziene, Saule; Kubiliene, Loreta; Bernatoniene, Jurga

    2016-01-01

    The aim of this study was to optimize the lipsticks formulation according to the physical properties and sensory attributes and investigate the relationship between instrumental and sensory analyses and evaluate the influence of the main ingredients, beeswax and oil, with analysis of lipsticks properties. Central composite design was used to optimize the mixture of oils and beeswax and cocoa butter for formulation of lipsticks. Antioxidant activity was evaluated by DPPH free radical scavenging method spectrophotometrically. Physical properties of lipsticks melting point were determined in a glass tube; the hardness was investigated with texture analyzer. Sensory analysis was performed with untrained volunteers. The optimized mixture of sea buckthorn oil and grapeseed oil mixture ratio 13.96 : 6.18 showed the highest antioxidative activity (70 ± 0.84%) and was chosen for lipstick formulation. According to the sensory and instrumental analysis results, optimal ingredients amounts for the lipstick were calculated: 57.67% mixture of oils, 19.58% beeswax, and 22.75% cocoa butter. Experimentally designed and optimized lipstick formulation had good physical properties and high scored sensory evaluation. Correlation analysis showed a significant relationship between sensory and instrumental evaluations.

  2. Skin Penetration Enhancement by Natural Oils for Dihydroquercetin Delivery.

    Science.gov (United States)

    Čižinauskas, Vytis; Elie, Nicolas; Brunelle, Alain; Briedis, Vitalis

    2017-09-12

    Natural oils are commonly used in topical pharmaceutical formulations as emulsifiers, stabilizers or solubility enhancers. They are presented as safe and inert components, mainly used for formulation purposes. It is confirmed that natural oils can affect the skin penetration of various substances. Fatty acids are mainly responsible for this effect. Current understanding lacks reliable scientific data on penetration of natural oils into the skin and their skin penetration enhancement potential. In the current study, fatty acid content analysis was used to determine the principal fatty acids in soybean, olive, avocado, sea-buckthorn pulp, raspberry seed and coconut oils. Time of flight secondary ion mass spectrometry bioimaging was used to determine the distribution of these fatty acids in human skin ex vivo after application of the oils. Skin penetration enhancement ratios were determined for a perspective antioxidant compound dihydroquercetin. The results demonstrated skin penetration of fatty acids from all oils tested. Only soybean and olive oils significantly increased the skin distribution of dihydroquercetin and can be used as skin penetration enhancers. However, no correlation can be determined between the fatty acids' composition and skin penetration enhancement using currently available methodological approaches. This indicates that potential chemical penetration enhancement should be evaluated during formulation of topically applied products containing natural oils.

  3. A revised molecular phylogeny of the globally distributed hawkmoth genus Hyles (Lepidoptera: Sphingidae), based on mitochondrial and nuclear DNA sequences.

    Science.gov (United States)

    Hundsdoerfer, Anna K; Rubinoff, Daniel; Attié, Marc; Wink, Michael; Kitching, Ian J

    2009-09-01

    The hawkmoth genus Hyles comprises some 29 species with a global distribution. In this study, we augment the previous taxon sampling with more species and add sequences from a nuclear gene to produce a refined phylogenetic hypothesis. A total evidence reconstruction based on Bayesian analysis of the combined mitochondrial (COI, t-RNA-Leu, COII; 2284 bp) and nuclear (EF1alpha; 773 bp) sequences is discussed and compared with the results from separate analyses of the two genes. The total evidence phylogeny corroborates many of the phylogenetic relationships previously postulated within the genus. In addition, the hitherto unsampled enigmatic species Hyles biguttata from Madagascar appears as sister group to Hyles livornicoides from Australia, although support for the relationship is relatively weak. The high level of differentiation of Hyles perkinsi from H. calida (both Hawaii), and the status of these two as sister species, is corroborated by both sources of sequence data. However, their phylogenetic position when mt DNA sequences alone are considered differs markedly from that under total evidence. The previously postulated relationships within the Hyles euphorbiae complex (HEC) s.s. are largely corroborated, but H. dahlii is now more closely related and the HEC s.l. is redefined to include H. zygophylli and H. stroehlei (two species that had not been studied previously using molecular data) and to exclude H. siehei and H. hippophaes. The nuclear sequences alone are insufficiently variable to fully resolve all lineages and the phylogeny suggests that nuclear gene swapping and incomplete lineage sorting have occurred implying recent divergence. The results from the total evidence analysis provide a phylogenetic hypothesis that both corroborates and complements the previous biogeographic scenario, and provides new insights into the origins of several of the included taxa.

  4. Evaluating the potential and opportunities for agroforestry in the Province of Saskatchewan

    Energy Technology Data Exchange (ETDEWEB)

    Mak, K.; Lavoie, A.; Grundberg, B. [KPMG Consulting, Edmonton, AB (Canada)

    1999-05-01

    Results of a recent evaluative study of the current agroforestry industry, its potential and market opportunities in Saskatchewan are discussed. Results show that in the absence of incentive programs like those established in Ontario, Quebec, the Atlantic provinces, British Columbia and especially in the United States, the practice of woodlot management in Saskatchewan is on a small scale and on an experimental basis; agroforestry activities have been limited primarily to the establishment of shelter-belts on farmland and grazing cattle in woodlots. Under the current situation, most of the agroforestry scenarios tested in this study appear to be of marginal profitability, although high value crops such as sea buckthorn, incorporated into shelter-belts can provide substantial returns and sustainable grazing practices could be used to produce slight increases in revenues. The main factors responsible for the marginal profitability are the oversupply of public timber in Saskatchewan, and the pricing structures under the current timber tenure system which leave little room for private land owners to compete. In the long run, the key to successful agroforestry industry in Saskatchewan is contingent upon a growing demand for timber, especially hardwood supply, and limited availability of public timber. To encourage the development of the industry it is recommended to review pricing structures of crown timber and create a more favorable pricing structure for private land owners; changing the income tax structure to make it more favorable to agroforestry; improving the economies of scale for woodlot producers by allowing them to integrate woodlots with crown land; providing extension services specialized in agroforestry to support woodlot owners; and establishing alternative funding mechanisms that would encourage landowners to invest in silviculture on their private forest land. 32 refs.

  5. A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis.

    Directory of Open Access Journals (Sweden)

    Magdalena Boncler

    Full Text Available The toxicity of in vitro tested compounds is usually evaluated based on AC50 values calculated from dose-response curves. However, there is a large group of compounds for which a standard four-parametric sigmoid curve fitting may be inappropriate for estimating AC50. In the present study, 22 polyphenol-rich compounds were prioritized from the least to the most toxic based on the total area under and over the dose-response curves (AUOC in relation to baselines. The studied compounds were ranked across three key cell indicators (mitochondrial membrane potential, cell membrane integrity and nuclear size in a panel of five cell lines (HepG2, Caco-2, A549, HMEC-1, and 3T3, using a high-content screening (HCS assay. Regarding AUOC score values, naringin (negative control was the least toxic phenolic compound. Aronox, spent hop extract and kale leaf extract had very low cytotoxicity with regard to mitochondrial membrane potential and cell membrane integrity, as well as nuclear morphology (nuclear area. Kaempferol (positive control exerted strong cytotoxic effects on the mitochondrial and nuclear compartments. Extracts from buckthorn bark, walnut husk and hollyhock flower were highly cytotoxic with regard to the mitochondrion and cell membrane, but not the nucleus. We propose an alternative algorithm for the screening of a large number of agents and for identifying those with adverse cellular effects at an early stage of drug discovery, using high content screening analysis. This approach should be recommended for series of compounds producing a non-sigmoidal cell response, and for agents with unknown toxicity or mechanisms of action.

  6. Land Desertification and it’s Control in Gonghe Basin of Qinghai Plateau, China

    Science.gov (United States)

    Zhang, D.; Gao, S.; Lu, R.

    2009-12-01

    seeding > Caragana direct seeding, Tamarix cutting and seedling > Salix cheilophila deep planting, Sea-buckthorn seedling > Tamarix deep planting > Tamarix seedling > Poplar deep planting > Salix cheilophila seedling > Poplar seedling. It has resolved the key problem of control sand flow speed and low efficiency, sand burying and wind erosion and low conservation rate for forestation in the sandy area.

  7. SORPTION OF CO2-EXTRACTS OF SPICES TO THE TOMATO PASTE

    Directory of Open Access Journals (Sweden)

    A. M. Gadzhieva

    2014-01-01

    Full Text Available The possibility of the production of products without the preservatives, the fillers, the chemical, undesirable aromatic substances and other additives is one of the prime advantages of technologies, based on the use of СO2- extraction. There is great theoretical and practical interest in the study of the absorption of СO2- extracts on the tomato carriers with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. The miscella of the extracts of spices is brought to the tomato paste. This was reached by the selection of СO2- miscella from the extractor under the pressure to 6 MPa. Then solvent due to a pressure decrease in the apparatus to the atmospheric instantly was moved away from the obtained material. The degrees of the absorption of the СO2- extracts of anise, ginger, amaranth and sea buckthorn are determined. Calculations were performed through the program of that composed in medium of the mathematical packet “Of mathCAD 14”. As a result conducted investigations are obtained the equations of regression for absorbing of anise, amaranth and ginger on the tomato paste depending on the concentration of individual extracts in the miscella. The possibilities of the method of complete factor experiment are shown. In the utilized method of the mathematical planning of experiment adapt the dimensionless coded composition factors x1 and x2. In the experiment is realized plan 23. Was used the central composition plan, which corresponds to the requirement of rotatable, i.e., the plan, which makes it possible to obtain the model, capable of predicting the value of the parameter of optimization with the

  8. Puccinia coronata f. sp. avenae: a threat to global oat production.

    Science.gov (United States)

    Nazareno, Eric S; Li, Feng; Smith, Madeleine; Park, Robert F; Kianian, Shahryar F; Figueroa, Melania

    2017-08-28

    ) and wild relatives such as bluejoint grass, perennial ryegrass, and fescue. Alternate hosts include several species of Rhamnus, with R. cathartica (common buckthorn) as the most important alternate host in Europe and North America. Most crown rust management strategies involve the use of rust-resistant crop varieties and application of fungicides. Attaining durability of resistance against Pca has been difficult for it is a highly variable pathogen with a great propensity to overcome the genetic resistance of varieties. Thus, adult plant resistance is often exploited in oat breeding programs to develop new crown rust-resistant varieties. Useful website: https://www.ars.usda.gov/midwest-area/st-paul-mn/cereal-disease-lab/docs/cereal-rusts/race-surveys/ This article is protected by copyright. All rights reserved. © 2017 BSPP and John Wiley & Sons Ltd.

  9. Comparison of ice nuclei from fruit juices and their properties

    Science.gov (United States)

    Fiala, Bianca; Felgitsch, Laura; Grothe, Hinrich

    2017-04-01

    Heterogeneous ice nucleation is a crucial process in the atmosphere. Recent findings indicate the importance of biological ice nuclei (IN) in this process. Pratt et al. (2009) sampled ice-crystal residues at approx. 8 km high altitude over Wyoming, U.S.. They found a third of the dry residues to be biological and further that 60% of the highly abundant mineral dusts to be internally mixed with biological or humic substances. Huffman et al. (2013) showed a burst of biological IN over woodlands connected to rain events. Previous investigations in our group (Pummer et al. 2012) showed that pollen and pollen washing water from several plants native to the boreal forests trigger heterogeneous ice nucleation. Recent work from our group (Felgitsch et al. 2016) showed that several juices of berries are ice nucleation active. Based on this research we examined ice nucleation activity (INA) and general properties of five juices: sea buckthorn, black currant, chokeberry, acerola, and elder berry. We elucidated particle size dependency of the ice nucleation activity to differentiate between coarse ice nucleating particles and nanoparticles or macromolecules. Investigations with different solvents and with chaotropic agents were performed in order to further clarify properties concerning chemical polarity of the IN, and the importance of hydrogen bonds and other structure forming polar interactions. The results are compared to known plant originated IN. Our results suggest similarities in terms of nucleation temperature and structure stability between different IN of plant materials. These properties show a clear differentiation to known bacterial and fungal IN. Felgitsch , L., Bichler, M., Häusler, T., Hitzenberger, R., and Grothe, H.: Heterogeneous freezing of water triggered by berry juices from perenneal plants, submitted, 2016. Huffman, J.A., Prenni, A.J., DeMott, P.J., Pöhlker, C., Mason, R.H., Robinson, N.H., Frohlich-Nowoisky, J., Tobo, Y., Després, V.R., Garcia, E

  10. Effects of invasive plant species on pollinator service and reproduction in native plants at Acadia National Park

    Science.gov (United States)

    Stubbs, C.J.; Drummond, F.; Ginsberg, H.

    2007-01-01

    Invasive plant species can have profound negative effects on natural communities by competively excluding native species. Berberis thunbergii (Japanese barberry), Frangula alnus (glossy or alder buckthorn) and Lythrum salicaria (purple loosestrife) are invasive species known to reduce native plant diversity and are thus of great concern to Acadia National Park. Pollinators visit them for nectar and pollen. The effects of invasive plant species on pollinator behavior were investigated by comparing pollinator visitation to co-flowering native and invasive species with visitation to native species growing alone. The effect of invasives on pollination of native plants was studied by comparing fruit set in patches of the native species growing near invasives with patches far from invasive species in Acadia National Park. The coflowering pairs were as follows: in the spring native Vaccinium angustifolium (lowbush blueberry) was paired with B. thunbergii; in early summer native Viburnum nudum (wild raisin) was paired with F. alnus ; in late summer native Spiraea alba (meadowsweet) was paired with L. salicaria. We investigated whether these invasives competed with native plants for pollinators in Acadia and thus negatively affected native plant reproduction. Our objectives were to determine: 1) the influence, if any, of each invasive on pollinator visitation to a co-flowering native species, 2) factors that might affect visitation, 3) invasive pollen transfer to native plants, and 4) whether invasives influence native plant reproduction (fruit set). Our findings indicate that at times the number of flower visitors to natives was lower or the species composition of visitors different when invasives were present, that invasives sometimes attracted more pollinators, that generally the invasives were more rewarding as far as nectar and pollen availability for pollinators, and that generally native plant fruit set and seed set was not significantly lowered in the presence of

  11. Phytotherapy of Acute Respiratory Viral Diseases

    Directory of Open Access Journals (Sweden)

    I.B. Ershova

    2016-11-01

    of acute respiratory viral infections the medicinal plants of several pharmacological groups mainly used are: plants with a tonic effect (plants containing vitamins and minerals — rose hips, black currant, sea buckthorn, citrus fruits, adaptogens and immune modulators should be used in pediatric practice with caution (Ginseng, Rhodiola Rosea, Aralia Manchurian, plants containing biogenic stimulators — the aloe latex, kalanchoe, plant with bactericidal and bacteriostatic effect (sage leaves drug, eucalyptus switchgrass, plants with anti-inflammatory effect, containing tannins — oak bark, Potentilla rhizome, rhizome of Polygonum snake, burnet, alder cones, etc; plants containing essential oils, azulene derivatives — Chamomile flowers; plants containing mucus — Farfara leaves, Plantain; Linden flowers; plants with antipyretic and diaphoretic action — plants containing vitamins (fruits of raspberry, black currant fruit, cranberry; plants containing salicylic acid derivatives — Viola tricolor, raspberry fruit; plants with protective, emollient, expectorant action containing mucus — Farfara leaves; Linden flowers, plantain leaves are large; containing alkaloids, with antitussive effect —codeine phosphate; glaucine hydrochloride grass of Thermopsis lancet; bronchodilators plants; plants with anti-allergic effect, containing flavonoids — herb of Viola tricolor, the roots of Licorice. The article presents the re­commendation of professor O.D. Barnaulov, a pediatric phytotherapist O.A. Daniluk on herbal medicine using in children, the rules of the certain medicinal plants use by Y.I. Korshikova, dosage regimen for herbal remedies for children, proposed by N.P. Menshikova, as well as practical advice on drafting phytospecies for the treatment and prophylaxis of acute respiratory viral infections and prescribing proposed by phytotherapist O.V. Kostareva.