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Sample records for buckthorn hippophae rhamnoides

  1. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L.) Seed against Visible Light-Induced Retinal Degeneration in Vivo.

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    Wang, Yong; Zhao, Liang; Huo, Yazhen; Zhou, Feng; Wu, Wei; Lu, Feng; Yang, Xue; Guo, Xiaoxuan; Chen, Peng; Deng, Qianchun; Ji, Baoping

    2016-05-02

    Dietary proanthocyanidins (PACs) as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L.) seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day) for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6) and angiogenesis (VEGF) levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  2. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L. Seed against Visible Light-Induced Retinal Degeneration in Vivo

    Directory of Open Access Journals (Sweden)

    Yong Wang

    2016-05-01

    Full Text Available Dietary proanthocyanidins (PACs as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L. seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6 and angiogenesis (VEGF levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  3. Data on coal dumps retrieving in petroşani basin using sea buckthorn (Hippophae rhamnoides

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    Andreea BRASOVAN

    2008-05-01

    Full Text Available This contribution presents data on the sea buckthorn (Hippöphae rhamnoides, with focus on its value on coal dumps retrieving in the Petroşani Basin. Due to its peculiar roots exposing a lot of nodules, this plant has an important role in fixing the atmospheric azoth and soil genesis. It is very adaptable to any kind of soil and has wide climatic exigencies, but is depending on light intensity. It has a large dissemination capacity, due to its light and numerous seeds. Besides its utilization on dump retrieving, sea buckthorn is used in medicine and pharmacy, as well as in organic fuel production.

  4. Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides, Apricot Pulp and Apricot Kernel (Prunus armeniaca

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    Elena Andreea Pop

    2015-11-01

    Full Text Available A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L. and apricot fruits (Prunus armeniaca L. are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits. The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC  method.  Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection. The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg than in apricot fruits (1.09 mg/kg while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg. Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.

  5. NMR metabolomics demonstrates phenotypic plasticity of sea buckthorn (Hippophaë rhamnoides) berries with respect to growth conditions in Finland and Canada.

    Science.gov (United States)

    Kortesniemi, Maaria; Sinkkonen, Jari; Yang, Baoru; Kallio, Heikki

    2017-03-15

    The berries of sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) cultivars 'Terhi' and 'Tytti' were studied with respect to their growth location, 60° and 68°N latitude in Finland and 46°N in Canada, using (1)H NMR metabolomics. The berries of 'Terhi' were characterised by stronger signals of quinic acid, while 'Tytti' had higher levels of O-ethyl β-d-glucopyranoside. The metabolic profile of the northernmost berries was distinctly different from those grown in southern Finland or Canada. Berries from northern Finland had relatively higher levels of quinic acid, glucose, l-quebrachitol and ascorbic acid. Ethyl glucoside was shown to accumulate by several fold at the late stage of maturation in the south as it correlated with degree days (r=0.63) and global radiation (r=0.59), but not in the north. The variance in the composition of the sea buckthorn berries demonstrates plasticity in the acclimatisation to growth environments.

  6. Effect of monospecific and mixed sea-buckthorn (Hippophae rhamnoides) plantations on the structure and activity of soil microbial communities.

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    Yu, Xuan; Liu, Xu; Zhao, Zhong; Liu, Jinliang; Zhang, Shunxiang

    2015-01-01

    This study aims to evaluate the effect of different afforestation models on soil microbial composition in the Loess Plateau in China. In particular, we determined soil physicochemical properties, enzyme activities, and microbial community structures in the top 0 cm to 10 cm soil underneath a pure Hippophae rhamnoides (SS) stand and three mixed stands, namely, H. rhamnoides and Robinia pseucdoacacia (SC), H. rhamnoides and Pinus tabulaeformis (SY), and H. rhamnoides and Platycladus orientalis (SB). Results showed that total organic carbon (TOC), total nitrogen, and ammonium (NH4(+)) contents were higher in SY and SB than in SS. The total microbial biomass, bacterial biomass, and Gram+ biomass of the three mixed stands were significantly higher than those of the pure stand. However, no significant difference was found in fungal biomass. Correlation analysis suggested that soil microbial communities are significantly and positively correlated with some chemical parameters of soil, such as TOC, total phosphorus, total potassium, available phosphorus, NH4(+) content, nitrate content (NH3(-)), and the enzyme activities of urease, peroxidase, and phosphatase. Principal component analysis showed that the microbial community structures of SB and SS could clearly be discriminated from each other and from the others, whereas SY and SC were similar. In conclusion, tree species indirectly but significantly affect soil microbial communities and enzyme activities through soil physicochemical properties. In addition, mixing P. tabulaeformis or P. orientalis in H. rhamnoides plantations is a suitable afforestation model in the Loess Plateau, because of significant positive effects on soil nutrient conditions, microbial community, and enzyme activities over pure plantations.

  7. Effects of genotype, latitude, and weather conditions on the composition of sugars, sugar alcohols, fruit acids, and ascorbic acid in sea buckthorn (Hippophaë rhamnoides ssp. mongolica) berry juice.

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    Zheng, Jie; Yang, Baoru; Trépanier, Martin; Kallio, Heikki

    2012-03-28

    Sea buckthorn berries (Hippophaë rhamnoides ssp. mongolica) of nine varieties were collected from three growth locations in five inconsecutive years (n = 152) to study the compositional differences of sugars, sugar alcohols, fruit acids, and ascorbic acid in berries of different genotypes. Fructose and glucose (major sugars) were highest in Chuiskaya and Vitaminaya among the varieties studied, respectively. Malic acid and quinic acid (major acids) were highest in Pertsik and Vitaminaya, respectively. Ascorbic acid was highest in Oranzhevaya and lowest in Vitaminaya. Berry samples of nine varieties collected from two growth locations in five years (n = 124) were combined to study the effects of latitude and weather conditions on the composition of H. rhamnoides ssp. mongolica. Sea buckthorn berries grown at lower latitude had higher levels of total sugar and sugar/acid ratio and a lower level of total acid and were supposed to have better sensory properties than those grown at higher latitude. Glucose, quinic acid, and ascorbic acid were hardly influenced by weather conditions. The other components showed various correlations with temperature, radiation, precipitation, and humidity variables. In addition, fructose, sucrose, and myo-inositol correlated positively with each other and showed negative correlation with malic acid on the basis of all the samples studied (n = 152).

  8. Thermal stability of the complex formed between carotenoids from sea buckthorn (Hippophae rhamnoides L.) and bovine β-lactoglobulin

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    Aprodu, Iuliana; Ursache, Florentina-Mihaela; Turturică, Mihaela; Râpeanu, Gabriela; Stănciuc, Nicoleta

    2017-02-01

    Sea buckthorn has gained importance as a versatile nutraceutical, due to its high nutritive value in terms of carotenoids content. β-Lactoglobulin (β-LG) is a natural carrier for various bioactive compounds. In this study, the effect of thermal treatment in the temperature range of 25 to 100 °C for 15 min on the complex formed by β-LG and carotenoids from sea buckthorn was reported, based on fluorescence spectroscopy, molecular docking and molecular dynamics simulation results. Also, the berries extracts were analyzed for their carotenoids content. The chromatographic profile of the sea buckthorn extracts revealed the presence of zeaxanthin and β-carotene, as major compounds. The Stern-Volmer constants and binding parameters between β-LG and β-carotene were estimated based on quenching experiments. When thermally treating the β-LG-carotenoids mixtures, an increase in intrinsic and extrinsic fluorescence intensity up to 90 °C was observed, together with blue-shifts in maximum emission in the lower temperature range and red-shifts at higher temperature. Based on fluorescence spectroscopy results, the unfolding of the protein molecules at high temperature was suggested. Detailed information obtained at atomic level revealed that events taking place in the complex heated at high temperature caused important changes in the β-carotene binding site, therefore leading to a more thermodynamically stable assembly. This study can be used to understand the changes occurring at molecular level that could help food operators to design new ingredients and functional foods, and to optimize the processing methods in order to obtain healthier food products.

  9. Use of electron beam irradiation to improve the microbiological safety of Hippophae rhamnoides

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    Minea, R.; Nemţanu, M. R.; Manea, S.; Mazilu, E.

    2007-09-01

    Sea buckthorn ( Hippophae rhamnoides) is increasingly used in food supplements due to its dietary and medicinal compounds with a beneficial role in human diet and health. As many other medicinal plants, sea buckthorn can be contaminated with microorganisms which exerts an important impact on the overall quality of the products. Irradiation is an effective method for food preservation because it is able to destroy pathogenic microorganisms keeping the organoleptic and nutritional characteristics of the foods. The objective of the present study was to investigate the application of electron beam irradiation in order to improve the microbiological safety of sea buckthorn. The experimental results indicated that the electron beam treatment might be a good method to remove undesirable microorganisms from sea buckthorn without significant changes in its active principles.

  10. UHPLC/PDA–ESI/MS Analysis of the Main Berry and Leaf Flavonol Glycosides from Different Carpathian Hippophaë rhamnoides L. Varieties

    NARCIS (Netherlands)

    Pop, R.M.; Socaciu, C.; Pintea, A.; Buzoianu, A.D.; Sanders, M.G.; Gruppen, H.; Vincken, J.P.

    2013-01-01

    Introduction - Sea buckthorn (Hippophaë rhamnoides L.) is known to be rich in many bioactive compounds (such as vitamins, phenolics, carotenoids) important for human health and nutrition. Among the phenolics, berries and leaves contain a wide range of flavonols that are good quality and authenticity

  11. Evaluation of antioxidant activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To investigate the antioxidant activity of aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane extracts of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Antioxidant activity was evaluated by using in-vitro antioxidant assays model 1, 1’-diphenyl-2-picrylhydrazyl radical-scavenging activity. The antioxidant activities were compared with standard antioxidant agents such as ascorbic acid. Results: The antioxidant activities (% inhibition) of all the tested extracts were increased in the order i.e. menthol > ethanol > aqueous > acetone > chloroform > ethyl acetate > n-hexane. The methanol extract EC50 (µg/mL) value was compatible with vitamin C (standard). The antioxidant activity of H. rhamnoides leaves extracts increased in a dose dependent manner. Conclusions: It was observed that H. rhamnoides was a potential resource of antioxidants and thus could put off numerous radical linked diseases.

  12. Associations of SRAP markers with dried-shrink disease resistance in a germplasm collection of sea buckthorn (Hippophae L.).

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    Li, He; Ruan, Cheng-Jiang; Teixeira da Silva, Jaime A; Liu, Bao-Quan

    2010-06-01

    Sea buckthorn (Hippophae L.) is a woody, outcrossing dioecious pioneer plant, being widely planted as a new berry crop with rich nutritional and medicinal compounds. This long-juvenile and long-lived woody plant can be more difficult to cultivate than other crop plants. Dried-shrink disease (DSD) is a dangerous pathogen that destroys sea buckthorn and halts commercial production. We estimated variability of sequence-related amplified polymorphism (SRAP) markers in 77 accessions of 22 sea buckthorn cultivars to seek markers associated with DSD resistance and help to identify potential breeding cultivars. Seventeen SRAP primer combinations generated 289 bands, with a mean of 17 bands per primer combination. At a Dice coefficient of 0.852, the dendrogram generated with 191 polymorphic bands clustered 73 accessions of Hippophae rhamnoides into 2 groups and 4 accessions of Hippophae salicifolia into 1 group. Eleven SRAP markers (Me1-Em3(600), Me1-Em1(680), Me2-Em1(650), Me2-Em1(950), Me3-Em6(1300), Me2-Em6(320), Me2-Em6(400), Me1-Em2(600), Me1-Em1(1200), Me1-Em1(1700), Me2-Em2(250)) were significantly correlated with DSD resistance (P < 0.001). These markers provide a viable option for breeding programs that select lineages with DSD resistance, especially when no other genetic information, such as linkage maps and quantitative trait loci, are available.

  13. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane) of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution) were transferred to specific concentration of each extract. Results: The mortality of aqueous extract was 46.7%, methanol extract was 46.7%, ethanolic extract was 50.0%, ethyl acetate was 26.7%, acetone extract was 33.3%, chloroform extract was 40.0% and n-hexane extract was 33.3%. The lowest LD50 was found in methanol extracts (1199.97 µg/mL). Brine shrimp cytotoxicity of tested extracts of H. rhamnoides showed that mortality rate was concentration dependent. Conclusions: It is concluded that bioactive components are present in all leaves extracts of H. rhamnoides, which could be accounted for its pharmacological effects. Thus, the results support the uses of this plant species in traditional medicine.

  14. The Evaluation of Synergistic Effect of Hippophae rhamnoides and Vitamin E on Growth Performance and Oxidative Stress at Oreochromis niloticus - Linnaeus, 1758

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    Alina Antache

    2014-11-01

    Full Text Available The aim of this research is to evaluate the influence of sea buckthorn (Hippophae rhamnoides and vitamin E on growth performance indicators and oxidative stress at Nile tilapia juvenile, reared in a recirculating aquaculture system. The experiment was conducted six weeks, in triplicate. The experimental variants were: V1 – control, V2 – 1% sea buckthorn / kg feed, V3 – 500mg vitamin E / kg feed and V4 – 1% sea buckthorn supplemented with 500 mg vitamin E / kg feed. During the experiment was performed an intermediary biometric measurement. Oxidative stress analysis consisted in determination of lipid peroxidation (MDA-malondialdehide and total antioxidant capacity (TAC from liver, tissue and gut. Results showed a good evolution of GR, FCR and SGR, during the experiment, in V4 – in which feed was supplemented with sea buckthorn and vitamin E. Based on the results obtained in variant V4, in liver and tissue, the oxidative stress was reduced. Regarding MDA and TAC, between experimental variants, were registered significant differences (p<0.05 at the level of tissue and gut. In conclusion, the research shows that sea buckthorn (1%/kg feed in combination with Vitamin E (500mg/kg feed has a synergistic effect on growth performance indicators and oxidative stress, at Oreochromis niloticus juvenile.

  15. Effects of Ultra-drying on Vigor and Physiological characteristics of Hippophae rhamnoides Seeds

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Studies of ultra-drying and accelerated aging on Hippophae rhamnoides seeds from the arid and semiarid areas of northwest China were conducted. The results showed that H. rhamnoides seeds had higher desiccation endurance. When seeds were desiccated to 26 g/kg, their germination percentage, vigor indices and enzyme activity could be retained. After 30-day accelerated aging, ultra-dried seeds had better anti-aging ability and storability compared to natural dried seeds, and the best choice for the MC of seed ...

  16. Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma.

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    Olas, Beata; Kontek, Bogdan; Malinowska, Paulina; Żuchowski, Jerzy; Stochmal, Anna

    2016-01-01

    Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) and the generation of superoxide anion (O2 (-∙)) in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin) were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals). The tested fraction of H. rhamnoides (0.5- 50 µg/mL; the incubation time: 15 and 60 min) inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2 (-∙) in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL). The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

  17. Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma

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    Beata Olas

    2016-01-01

    Full Text Available Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation and the generation of superoxide anion (O2-∙ in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2 or H2O2/Fe (a donor of hydroxyl radicals. The tested fraction of H. rhamnoides (0.5– 50 µg/mL; the incubation time: 15 and 60 min inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2-∙ in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL. The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

  18. [Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation].

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    Yang, Dan; Yu, Xuan; Liu, Xu; Liu, Jin-liana; Zhang, Shun-xiang; Yu, Ze-qun

    2015-12-01

    The study aimed to assess the effect of different afforestation modes on microbial composition and nitrogen functional genes in soil. Soil samples from a pure Hippophae rhamnoides stand (SS) and three mixed stands, namely, H. rhamnoides and Pinus tabuliformis (SY), H. rhamnoides and Platycladus orientalis (SB), H. rhamnoides and Robinia pseucdoacacia (SC) were selected. The results showed that the total PLFA (TPLFA), bacterial PLFA, gram positive bacterial PLFA (G⁺PLFA) were significantly higher in soil samples from other three stands than those of the pure one. However, no significant difference was found for fungal PLFA among them. The abundance of nifH, amoA, nirK and narG genes were higher in SY and SC than in SS. The TPLFA, G⁺PLFA, gram negative bacterial PLFA (G⁻PLFA), and all of the detected gene abundance were significantly and positively correlated with soil pH, total organic carbon, total nitrogen, ammonium nitrogen and available potassium. Afforestation modes affected indirectly soil microbial composition and functional genes through soil properties. Mixing P. tabuliformis or P. orientalis with H. rhamnoides might be suitable afforestation modes, which might improve soil quality.

  19. Volatile compounds of healthy and insect-damaged Hippophae rhamnoides sinensis in natural and planted forests.

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    Zong, Shixiang; Luo, Youqing; Zhou, Jiao; Liu, Shujing

    2012-01-01

    Volatile compounds of healthy and insect-damaged stems of Hippophae rhamnoides sinensis were analysed using dynamic headspace and thermal-desorption cold-trap injector gas chromatography/mass spectroscopy (TCT-GC/MS). Sixteen compounds, belonging to alkanes, alcohols, aldehydes, esters, ketones, and ethers, were identified in the stems of healthy H. rhamnoides sinensis; the compounds in H. rhamnoides sinensis occurring naturally or cultivated in plantations were similar, but the relative contents were significantly different. In plants damaged by Holcocerus hippophaecolus, the nature and content of the volatile compounds were greatly changed. Butanedione and butyl glyoxylate were newly generated after damage by the pest, and the relative levels of pentanal, heptanal, eucalyptol, terpineol, and camphor were sharply increased in both naturally occurring and plantation-grown plants. n-Decane, trans-2-nonen-1-ol, and n-hexadecane levels increased in plants cultivated in the plantation and decreased in natural forests, whereas the levels of other types were reduced. Thus, both the nature and the content of volatile compounds of H. rhamnoides sinensis are affected by H. hippophaecolus damage, providing a theoretical basis to identify the mechanism of pest destruction.

  20. Fruits foraging patterns and seed dispersal effect of frugivorous birds on Hippophae rhamnoides sinensis

    Institute of Scientific and Technical Information of China (English)

    LU Xianwen; SUN Kun; MA Ruijun; ZHANG Hui; SU Xue; WANG Mingli

    2006-01-01

    Behaviors of 18 species of birds eating fruits of Hippophae rhamnoides spp.sinensis were observed from September 2003 to March 2004.Their foraging patterns were found to be very different and Can be divided into five classes:(1)direct swallowing the fruits on crown of the shrubs and sometimes regurgitating seeds soon after;(2)carrying the fruits to their perching sites and swallowing;(3)pecking the fruits from the shrubs to the ground,eating pulp and seeds but leaving pericarp;(4)pecking through the pericarp,eating pulp and leaving pericarp and seeds;(5)pecking through the pericarp on the top of fruits,and only eating seeds.These foraging patterns have different effects on seed dispersal of H.rhamnoides spp.sinensis.The germination experiment of three groups of seeds(seeds from feces,dry fruits and extracted seeds from dry fruits)was carried out.Although ingestion processes of birds had some adverse effects on the seed germination of H.rhamnoides spp.sinensis,the seeds from feces still have a relatively higher germination ratio.H.rhamnoides spp.sinensis provides food to a variety of frugivorous birds.and the birds disperse its seeds.Thus,a mutually beneficial relationship between the bird and the seed is formed.

  1. Influence of ambient and enhanced ultraviolet-B radiation on the plant growth and physiological properties in two contrasting populations of Hippophae rhamnoides.

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    Yang, Yongqing; Yao, Yinan; He, Hai

    2008-07-01

    Two contrasting sea buckthorn (Hippophae rhamnoides L.) populations from low and high altitude regions were employed to investigate the effects of prevailing and enhanced ultraviolet-B (UV-B) radiation on plant growth and physiological properties under a UV-B-enhanced/exclusion system. The experimental design included three UV-B regimes, including excluded (-UVB), near-ambient (NA) and enhanced UV-B (+UVB) radiation. Compared with the control (-UVB), NA caused the formation of smaller but thicker plant leaves in both sea buckthorn populations, paralleled with significant increments of carotenoids and UV-absorbing compounds as well as improved water economy. NA also induced more biomass partition from shoot to root, but CO(2) assimilation rate (A), photosynthetic area and biomass accumulation were unaffected. The low-altitude population seemed sensitive to +UVB, as indicated by the decreases in total biomass, A and ascorbic acid content (Asa, an antioxidant) compared with NA. However, little +UVB effect occurred on the high-altitude population, and we suggest that the higher tolerance of this population could be associated with its specific morphological and physiological characteristics, such as small but thick leaves and high-level of Asa content, as well as its greater physiological modification in response to NA, e.g., increases in protective compounds (carotenoids and UV-absorbing compounds) and improvement in water economy, in comparison to the low-altitude population, which form an effective adaptation strategy to enhanced UV-B stress.

  2. Influence of hippophae rhamnoides on two appetite factors, gastric emptying and metabolic parameters, in children with functional dyspepsia.

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    Xiao, Mantian; Qiu, Xiaoshan; Yue, Dianchao; Cai, Yongyi; Mo, Qingping

    2013-01-01

    Our aim was to explore in children with functional dyspepsia the effect of hippophae rhamnoides on the levels of plasma appetite factors and on their gastrointestinal motility. A hundred and twenty children with functional dyspepsia were randomly divided into three groups: Group I (treated with hippophae rhamnoides), Group II (treated with domperidone), and Group III (treated with hippophae rhamnoides plus domperidone). The treatment lasted for eight weeks. The levels of plasma leptin (LP) and neuropeptide Y (NPY) were measured before and after treatment. All patients underwent a gastric emptying (GE) test by ultrasound (US) to measure the rate of postprandial gastric antrum residual, at 30min, 60min, 90min and 120min. The average value of subcutaneous fat, body fat percentage, upper arm girth and body mass index (BMI) were also measured. To compare the US with the radionuclide GE test 14 healthy adults volunteers were tested by both GE techniques. We found that the levels of LP and NPY in plasma were markedly higher after treatment in Groups I and III than in Group II. The postprandial gastric antrum remains at 60min, 90min and 120min in Groups I and III fell greatly and the thickness of skin fold (SF), body fat percentage and arm girth increased (P0.05). In conclusion, in children's functional dyspepsia, our study showed that hippophae rhamnoides increases the levels of appetite factors, leptin and neuropeptide Y, increases gastric emptying and gastrointestinal digestive function, children's growth and development.

  3. 营养型沙棘果醋的研制%Development of Nutrition Fruit Vinegar of Hippophae rhamnoides

    Institute of Scientific and Technical Information of China (English)

    高晓松; 万永青; 韩健燿; 赵宏军

    2012-01-01

    沙棘醋因含有多种维生素、有机酸、无机盐、黄酮、胡萝卜素等生命活性成分,是果醋中的珍品,倍受人们的推崇和喜爱。目前沙棘醋的生产工艺中酒精发酵阶段基本都采用产酒干酵母,沙棘的特有风味难以得到充分体现。另外,为加快生产普遍采用较高的发酵温度,使沙棘果富含的水溶性维生素不易很好保存。针对这些问题,本文采用利用从沙棘果表面分离的单一酵母菌原汁发酵法酿造产生酒精、利用恶臭醋酸杆菌的混合变种菌对沙棘果酒进行醋酸发酵制作沙棘醋、对制作出的沙棘醋进行勾兑等新方法,从沙棘果上自行分离纯化并经过DNA测序鉴定的产香酵母作为酒精发酵的酵母菌种,采用低温发酵工艺,研究一种沙棘醋生产的新工艺路线。%With the rapid development of fruit vinegar,deep processing of Hippophae rhamnoides has been widely concerned.There are many active ingredients in fruit vinegar of Hippophae rhamnoides,such as vitamins,organic acid,inorganic salt,flavonoids and carotene.At present,dry yeast is used during the alcohol fermentation in production process of fruit vinegar of Hippophae rhamnoides,but the unique flavor of Hippophae rhamnoides can't be smelt.In addition,the water-soluble vitamins in Hippophae rhamnoides can't be preserved after higher fermentation temperature.In this paper,several new kinds of producing fruit vinegar of Hippophae rhamnoides have been adopted,they are separation of single yeast from the surface of Hippophae rhamnoides,acetic acid fermentation by use of mixed varieties bacteris,blending the newly-produced fruit vinegar of Hippophae rhamnoides.The aroma-producing yeast isolated and purified from Hippophae rhamnoides by itself and determined after DNA sequencing is taken as the yeast spercies of alcohol fermentation with low temperature fermentation,which is a new process for producing fruit vinegar of Hippophae rhamnoides.

  4. Comparison of isorhamnetin absorption properties in total flavones of Hippophae rhamnoides L. with its pure form in a Caco-2 cell model mediated by multidrug resistance-associated protein.

    Science.gov (United States)

    Xie, Yan; Duan, Jingze; Fu, Qingxue; Xia, Mengxin; Zhang, Lei; Li, Guowen; Wu, Tao; Ji, Guang

    2015-06-20

    Total flavones of Hippophae rhamnoides L. (TFH) are extracted from the widely distributed thorny bush Sea buckthorn (Hippophae rhamnoides L.). Isorhamnetin (IS) is one of the representative ingredients in TFH. In this study, the absorption properties of IS in TFH and its pure form were compared through transepithelial transport and cellular uptake experiments in a Caco-2 cell model. Our results show that the absorption properties of IS in TFH and its pure form were remarkably different: (1) Both PappAB and PappBA of IS in TFH were dramatically increased compared with those of IS pure form; consequently, its Pratio was 2.3-fold higher than that of IS; (2) Both the accumulation and efflux of IS in TFH were significantly enhanced compared with the single compound. One likely reason for these differences is that the multiple components in TFH significantly down regulated the mRNA expression level of MRP2, which lead to a decrease in the protein level of MRP2, based on western blotting and RT-PCR assays. This study highlights the significant differences in the absorption properties of flavonoid components in different forms and the potential multi-component interactions in TFH.

  5. [Determination of isorhamnetin in Hippophae rhamnoides Linn from West Sichuan plateau using near infrared diffuse reflectance spectroscopy].

    Science.gov (United States)

    Ye, Li-Ming; Zhou, Min; Zhang, Hao; Chen, Chu; Li, Zhang-Wan; Chen, Cong; Wang, Yan-Ping

    2008-02-01

    The objective of this study was to develop a method for the determination of isorhamnetin in Hippophae rhamnoides Linn from West Sichuan plateau using near infrared diffuse reflectance spectroscopy. Applying the method of mixing with SiO2, the near infrared spectra (NIS) with the range of 12 000-4 000 cm(-1) were recorded for the Hippophae rhamnoides Linn containing isorhamnetin with the content of 0.1%-0.8%. Calibration models were established using the PLS (partial least squares). Different spectra pretreatments methods were compared. The study showed that spectral information can be extracted thoroughly by constant offset elimination (COE) pretreatments method with the correlation coefficient (r2) of 0.739 8, SEC of 0.107 (standard deviation of the calibration sets) and SEP of 0.073 (standard deviation of the prediction sets). The results indicate that near infrared diffuse reflectance spectroscopy is more rapid and convenient than conventional methods.

  6. Comparative antitumor and anti-proliferative activities ofHippophae rhamnoidesL. leaves extracts

    Institute of Scientific and Technical Information of China (English)

    Javid Ali; Bashir Ahmad

    2015-01-01

    Objective:To evaluate the antitumor and anti-proliferative activities of methanol, aqueous, acetone, ethyl acetate, ethanol, chloroform andn-hexane extracts ofHippophae rhamnoides leaves. Methods: Antitumor activities were evaluated by using the antitumor potato disc assay by using inoculums (Agrobacterium tumefaciens) with three different concentrations of test samples (10, 100 and 1 000 mg/L). Anti-proliferative activity was evaluated by the given method of methyl thiazolyl tetrazolium assay. The concentrations of the extract ranging from 0.039 to 10 mg/mL were tested against HeLa cells. Results: Highest tumors inhibition activity (60.9% and 55.8%) was shown by methanol and ethanol extracts, with EC50 values of 424.41 and 434.61 mg/L respectively. At 10 mg/mL, The highest cell inhibition 75.61% was observed in methanol extract and the lowest 36.59% were calculated inn-hexane extract. The difference in tumor and cell inhibition (%) may be due to the different concentration of active compounds responsible for antitumor and anti-proliferative activities. All extracts have considerable level of tumor and cell inhibitiory effect in a dose dependent manner. Conclusions:Our finding showed thatHippophae rhamnoidesleaves are a potent natural source of antitumor and antiproliferative agent.

  7. Impacts of soil petroleum contamination on nutrient release during litter decomposition of Hippophae rhamnoides.

    Science.gov (United States)

    Zhang, Xiaoxi; Liu, Zengwen; Luc, Nhu Trung; Yu, Qi; Liu, Xiaobo; Liang, Xiao

    2016-03-01

    Petroleum exploitation causes contamination of shrub lands close to oil wells. Soil petroleum contamination affects nutrient release during the litter decomposition of shrubs, which influences nutrient recycling and the maintenance of soil fertility. Hence, this contamination may reduce the long-term growth and stability of shrub communities and consequently, the effects of phytoremediation. Fresh foliar litter of Hippophae rhamnoides, a potential phytoremediating species, was collected for this study. The litter was placed in litterbags and then buried in different petroleum-polluted soil media (the petroleum concentrations were 15, 30, and 45 g kg(-1) dry soil, which were considered as slightly, moderately and seriously polluted soil, respectively) for a decomposition test. The impacts of petroleum contamination on the release of nutrients (including N, P, K, Cu, Zn, Fe, Mn, Ca and Mg) were assessed. The results showed that (1) after one year of decomposition, the release of all nutrients was accelerated in the slightly polluted soil. In the moderately polluted soil, P release was accelerated, while Cu, Zn and Mn release was inhibited. In the seriously polluted soil, Cu and Zn release was accelerated, while the release of the other nutrients was inhibited. (2) The effect of petroleum on nutrient release from litter differed in different periods during decomposition; this was mainly due to changes in soil microorganisms and enzymes under the stress of petroleum contamination. (3) To maintain the nutrient cycling and the soil fertility of shrub lands, H. rhamnoides is only suitable for phytoremediation of soils containing less than 30 g kg(-1) of petroleum.

  8. Sea buckthorn as a source of important bioactive compounds in cardiovascular diseases.

    Science.gov (United States)

    Olas, Beata

    2016-11-01

    Hippophae rhamnoides (sea buckthorn) offers many health benefits. It has significant cardioprotective activity and exerts many positive healing effects on the cardiovascular system, including inhibiting blood platelet activation (especially platelet aggregation), lowering cholesterol concentration and blood pressure, and providing antioxidant activity. In addition, sea buckthorn has antibacterial and antiviral properties. The leaves and fruits of the plant, and its oils, are sources of many bioactive substances including vitamins (A, C and E), unsaturated fatty acids, phenolic compounds, especially flavonoids, and phytosterols, which bestow positive effects on the cardiovascular system. This review article summarizes the current knowledge of the biological roles of sea buckthorn in cardiovascular diseases.

  9. Flavonoids from Hippophae Rhamnoides L.subsp.sinensis%中国沙棘果实黄酮成分的研究

    Institute of Scientific and Technical Information of China (English)

    高锦明; 张鞍灵

    1999-01-01

    从中国沙棘Hippophae rhamnoides L. subsp. sinensis Rousi果实中分到3种黄酮化合物,经光谱法和化学反应分别鉴定为槲皮素(quercetin,1),山奈酚(Kaempferol,2)和芦丁(rutin,3).

  10. Spatial distribution of Holcocerus hippophaecolus (Lepidopetera: Cossidae) pupae in a seabuckthorn (Hippophae rhamnoides) stand.

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The seabuckthorn carpenter moth,Holcocerus hippophaecolus,which has a generation time of four years,is recently becoming one of the major pests of the seabuckthorn (Hippophae rhamnoides) in Inner Mongolia,Liaoning,Shanxi,Ningxia and Shaanxi of China (Hua et al.,1990).The larvae of the H.hippophaecolus mainly damage the stems and roots of the seabuckthorn,and the mature larvae pupate in the soil.The spatial distribution of the pupae was analyzed by using biostatistics and geostatistics in order to effectively control the insect and further study the spatial distribution of the population.Results show that most of the pupae (90%) had an eclosion time span from early June to the end of July.The sex ratio of the pupae was nearly 1:1 in the woodland samples.In addition,24.3% of the 971 trees investigated had pupae and it ranged from 0 to 4 per tree within a distance of 1.3 m from the base of the stem.90% of the pupae were aggregated within a distance of 1 m from the base of the stem.The pupae show intense spatial aggregation in the sampled woodland which had an 11.1 m spatial dependence and a 90.7% intensity in the local spatial continuity.Moreover,the population presented an intensive spotted distribution and many aggregated spots were found in the woodlands.As for the relationship between grid size and variogram of the pupae,the variations in the range,the intensity of local spatial continuity and the sill were all very low or non-existent when the grid size was 5 m,6 m or 7 m.Whereas,the value of the decisive coefficient was the biggest when the grid size was 5 m making it the ideal grid size.

  11. Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC).

    Science.gov (United States)

    Sharma, Upendra K; Sharma, Kapil; Sharma, Nandini; Sharma, Abhishek; Singh, Harsh P; Sinha, Arun K

    2008-01-23

    The outcome of different extraction procedures (microwave, ultrasound, Soxhlet, and maceration) on the antioxidant activity of seeds, leaves, pulp, and fruits of Hippophae rhamnoides (sea buckthorn or SBT) was investigated by two different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The SBT extracts were found to possess strong antioxidant activity measured in terms of TEAC (2.03-182.13 and 6.97-282.75 mg/g) with ABTS and DPPH assays, respectively. In general, the antioxidant capacity of microwave-assisted extracts was found to be significantly higher than those obtained by ultrasound-assisted extraction (UAE) and maceration while being slightly higher than Soxhlet extracts. Further, microwave extracts of seeds were found to possess maximum antioxidant capacity followed by leaves, fruits, and pulp. Also, the chemical composition of extracts, studied in terms of the total phenolic content, was found to be in the range of 1.9-23.5 mg/g Gallic acid equivalent (GAE), which indicates a strong correlation between antioxidant activity and phenolic content present in the SBT. In addition, some of its bioactive phenolic constituents, such as rutin ( 1), quercetin-3- O-galactoside ( 2), quercetin ( 3), myricetin ( 4), kaempferol ( 5), and isorhamnetin ( 6), were also quantified in different extracts by reverse-phase high-performance liquid chromatography (RP-HPLC).

  12. In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402 cells.

    Science.gov (United States)

    Teng, Bao-Song; Lu, Yan-Hua; Wang, Zheng-Tao; Tao, Xin-Yi; Wei, Dong-Zhi

    2006-09-01

    Isorhamnetin, a flavonol aglycone, isolated from the traditional Chinese medicine Hippophae rhamnoides L., was investigated in its cytotoxicity and its influence on human hepatocellular carcinoma cells (BEL-7402). The cytotoxic effects of isorhamnetin showed dose- and time-dependency against BEL-7402 cells, with IC(50) equal to 74.4+/-1.13 microg ml(-1) after treatment with isorhamnetin for 72 h. Cytotoxicity of the flavonols on tumor cells depends on cellular accumulation of the drugs. The amount of isorhamnetin accumulated in BEL-7402 cells was assayed by high-performance liquid chromatography (HPLC) and showed that isorhamnetin could permeate the cell membrane into the cell. Staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the cell treated with 50 microg ml(-1) isorhamnetin for 48 h. Flow cytometric analysis was performed to determine hypodiploid cells. The results of flow cytometry assay indicated that the percentage of hypodiploid BEL-7402 cells were 13.77+/-1.05% after 48 h treatment with 50 microg ml(-1) isorhamnetin. The treatment resulted in the appearance of a hypodiploid peak (sub-G(0)/G(1) peak), probably due to the presence of cells in apoptosis and apoptotic bodies with DNA content less than 2n. To our knowledge, this is the first report against human hepatocellular carcinoma cells (BEL-7402) of isorhamnetin.

  13. Protective effect of total flavonoids of seabuckthorn (Hippophae rhamnoides) in simulated high-altitude polycythemia in rats.

    Science.gov (United States)

    Zhou, Ji-Yin; Zhou, Shi-Wen; Du, Xiao-Huang; Zeng, Sheng-Ya

    2012-09-28

    Seabuckthorn (Hippophae rhamnoides L.) has been used to treat high altitude diseases. The effects of five-week treatment with total flavonoids of seabuckthorn (35, 70, 140 mg/kg, ig) on cobalt chloride (5.5 mg/kg, ip)- and hypobaric chamber (simulating 5,000 m)-induced high-altitude polycythemia in rats were measured. Total flavonoids decreased red blood cell number, hemoglobin, hematocrit, mean corpuscular hemoglobin levels, span of red blood cell electrophoretic mobility, aggregation index of red blood cell, plasma viscosity, whole blood viscosity, and increased deformation index of red blood cell, erythropoietin level in serum. Total flavonoids increased pH, pO₂, Sp(O₂), pCO₂ levels in arterial blood, and increased Na⁺, HCO₃⁻, Cl⁻, but decreased K⁺ concentrations. Total flavonoids increased mean arterial pressure, left ventricular systolic pressure, end-diastolic pressure, maximal rate of rise and decrease, decreased heart rate and protected right ventricle morphology. Changes in hemodynamic, hematologic parameters, and erythropoietin content suggest that administration of total flavonoids from seabuckthorn may be useful in the prevention of high altitude polycythaemia in rats.

  14. Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

    Science.gov (United States)

    Puterova, Janka; Razumova, Olga; Martinek, Tomas; Alexandrov, Oleg; Divashuk, Mikhail; Kubat, Zdenek; Hobza, Roman; Karlov, Gennady

    2017-01-01

    Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes. PMID:28057732

  15. A comparison of the pharmacokinetics of three different preparations of total flavones of Hippophae rhamnoides in beagle dogs after oral administration.

    Science.gov (United States)

    Duan, Jingze; Dang, Yang; Meng, Houjun; Wang, Huizhen; Ma, Ping; Li, Guowen; Wu, Tao; Xie, Yan

    2016-06-01

    Pharmacokinetic properties of isorhamnetin, quercetin, and kaempferol in three different total flavones of Hippophae rhamnoides (TFH) preparations were compared after oral administration to beagle dogs by a UPLC-MS method. The pharmacokinetic results showed that C max of isorhamnetin and quercetin in TFH solid dispersion (TFH-SD) and TFH self-emulsifying (TFH-SE) preparations was significantly enhanced than that in TFH preparations (p beagle dogs can be significantly increased in TFH-SD and TFH-SE preparations compared to TFH preparations, which was helpful to explore the new forms for oral administration TFH and explain their in vivo processes.

  16. Characteristics and simulation of the canopy conductance of Hippophae rhamnoides in Qaidam Region of northwestern China%柴达木地区沙棘冠层导度特征及模拟

    Institute of Scientific and Technical Information of China (English)

    王辉; 贺康宁; 徐特; 刘玉娟; 刘颖; 张雪

    2015-01-01

    为了揭示沙棘冠层导度对环境因子的响应规律,评价Jarvis模型在沙棘冠层尺度上的适用性,利用Granier热消散式探针连续测定了青海省柴达木盆地实验站内的沙棘灌丛树干液流,并同步观测气象因子,利用 Penman-Monteith方程反推方法,获得了长期连续的冠层导度。在分析沙棘冠层导度动态变化规律的基础上,采用十字交叉法对Jarvis模型进行参数率定和误差分析。结果表明:研究区沙棘冠层导度日变化呈“单峰型”曲线,冠层导度随饱和水汽压差的增加呈负指数关系下降,并与太阳辐射强度呈正相关。基于饱和水汽压差、太阳辐射强度和气温这3个环境变量的Jarvis模型可解释gc变化的81%,且最低相对误差仅11.01%。环境因子对冠层导度( gc )模型精度的影响依次为饱和水汽压差(ⅤPD)>太阳辐射强度( Rs )>气温( T)。%The aim of this study was to explain the response of canopy conductance of sea buckthorn ( Hippophae rhamnoides ) to different environmental conditions so as to evaluate the feasibility of application of Jarvis model in sea buckthorn at the canopy scale. By using Granier’ s thermal dissipation probe, the sap flow of sea buckthorn in Qaidam Basin in Qinghai Province, northwestern China was continuously measured,and as well,the environmental factors such as incoming solar radiation intensity (Rs), air temperature (T), relative humidity (RH), wind speed (u) and rainfall were synchronically measured. Based on sap flow, canopy conductance ( gc ) was continuously simulated by back-calculated Penman-Monteith model. By analysis of gc of sea buckthorn, Jarvis stomatal model was simulated and analyzed with cross-validation. The results indicated that the diurnal variation in canopy conductance of sea buckthorn showed a single-peaked curve. There was a negative logarithm relationship between canopy conductance and vapor pressure deficit (ⅤPD ) under

  17. DeepSAGE based differential gene expression analysis under cold and freeze stress in seabuckthorn (Hippophae rhamnoides L..

    Directory of Open Access Journals (Sweden)

    Saurabh Chaudhary

    Full Text Available Seabuckthorn (Hippophae rhamnoides L., an important plant species of Indian Himalayas, is well known for its immense medicinal and nutritional value. The plant has the ability to sustain growth in harsh environments of extreme temperatures, drought and salinity. We employed DeepSAGE, a tag based approach, to identify differentially expressed genes under cold and freeze stress in seabuckthorn. In total 36.2 million raw tags including 13.9 million distinct tags were generated using Illumina sequencing platform for three leaf tissue libraries including control (CON, cold stress (CS and freeze stress (FS. After discarding low quality tags, 35.5 million clean tags including 7 million distinct clean tags were obtained. In all, 11922 differentially expressed genes (DEGs including 6539 up regulated and 5383 down regulated genes were identified in three comparative setups i.e. CON vs CS, CON vs FS and CS vs FS. Gene ontology and KEGG pathway analysis were performed to assign gene ontology term to DEGs and ascertain their biological functions. DEGs were mapped back to our existing seabuckthorn transcriptome assembly comprising of 88,297 putative unigenes leading to the identification of 428 cold and freeze stress responsive genes. Expression of randomly selected 22 DEGs was validated using qRT-PCR that further supported our DeepSAGE results. The present study provided a comprehensive view of global gene expression profile of seabuckthorn under cold and freeze stresses. The DeepSAGE data could also serve as a valuable resource for further functional genomics studies aiming selection of candidate genes for development of abiotic stress tolerant transgenic plants.

  18. Antibacterial properties of Hippophae rhamnoides marc flavones%沙棘果皮渣黄酮抑菌活性研究

    Institute of Scientific and Technical Information of China (English)

    张凤清; 张东生

    2012-01-01

    Antibacterial properties of Hippophae rhamnoides marc flavones to Escherichia coli,Staphylococcus aureus,Saccharomyces cerevisiae,Proteus vuigaris,Enterobacter aerogenes,Streptococcus thermophilus,Bacillus subtilis,A.flavus,Penicillium sp.,Aspergillus niger which were harmful to sanitation and safety of food were investigated in this study,we also determine the minimal inhibitory concentration(MIC) of the Hippophae rhamnoides marc flavones.The result shows that each sensitive strain can be restrained well by the text substance,and the MIC was respectively determined as 5,20,40,40,80,5,2.5,20,10 mg/mL.%考查沙棘果皮渣黄酮对于大肠杆菌、金黄色葡萄球菌、酵母菌、变形杆菌、产气杆菌、嗜热链球菌、枯草芽孢杆菌、根霉、青霉和黑曲霉等几种主要危害食品卫生、安全菌种的抑制作用,并对其最低抑菌浓度进行测定。结果表明:沙棘果皮渣黄酮对大肠杆菌、金黄色葡萄球菌、酵母菌、变形杆菌、嗜热链球菌、枯草芽孢杆菌、根霉、青霉、黑曲霉均有明显抑菌效果,对各个菌种的最低抑菌浓度分别为5、20、40、40、80、5、2.5、20、10mg/mL。

  19. Solute-matrix and Solute-Solute Interactions during Supercritical Fluid Extraction of Sea Buckthorn Leaves

    OpenAIRE

    Sajfrtová, M. (Marie); Sovová, H. (Helena)

    2012-01-01

    In this work, the SFE was applied to extract selected medicinal substances from sea buckthorn (Hippophae rhamnoides L.) leaves at different conditions (pressure 20-28 MPa, temperature 40-60 °C and ethanol concentration in CO2 0-6.9 wt. %) influencing solvent power of CO2. Interest was focused on the leaf oleoresin (total extract) and its minor components (fat soluble vitamins and carotenoids). The yield of polar component was still steadily increasing at the moment when the extraction is al...

  20. 沙棘籽油微波提取的实验研究%Study on Extraction of Seed Oil of Hippophae rhamnoides L.by Microwave Technology

    Institute of Scientific and Technical Information of China (English)

    刘红波; 温娜; 周明科; 唐志书; 宋忠兴; 梁燕妮; 刘世军

    2016-01-01

    目的:研究与优化微波技术提取沙棘籽油的工艺.方法:以提油率为指标,以微波功率、微波时间、液料比为考察因素,利用正交设计法筛选优化微波提取沙棘籽油的提取工艺.结果:微波提取沙棘籽油的最佳工艺条件是:微波功率539 W,微波时间7 min,液料比8.此工艺条件下沙棘籽油的提取率为89.36%.结论:相比于传统提取方法,微波提取技术具有提取效果好,提取效率高的特点,可将微波提取技术应用到沙棘籽油的提取工艺中以提高产率、降低提取时间.%Objective:To study and optimize the extraction process of see d oil of Hippophae rhamnoides L .by microwave extrac-tion technology .Methods:The orthogonal design method was used to optimize the extraction process seed oil of Hippophae rham -noides L .by microwave extraction technology with the oil yield as the index and microwave power , microwave time and liquid to solid ratio were taken as study factors .Results:The optimum extraction process was as follows:microwave power at 539 W, mi-crowave time at 7 min and liquid to solid ratio at 8.The extraction rate of oil was 89.36%under this extraction conditions .Con-clusion:Microwave extraction technology may have good extraction yield and high extraction efficiency compared with traditional extraction methods .It is suitable to apply microwave technology into the extraction process of seed oil of Hippophae rhamnoides L . with the purpose of increasing the yield and reducing the extraction time .

  1. 种衣剂拌种对沙棘种子繁苗效果的影响%Effects of Seed Coating Agent with Mixed Seeds on the Propagation of Hippophae rhamnoides Seeds

    Institute of Scientific and Technical Information of China (English)

    李志武

    2014-01-01

    Seedling-raising experiments of Hippophae rhamnoide were conducted by mixed seed coating agent . Growth & yield of Hippophae rhamnoide seedlings in the very Autumn were investigated .The effects of seed coat-ing agent on propagation of Hippophae rhamnoides seedlings were discussed .Result shows that :seed coating a-gent with mixed seeds can significantly increase yield of Hippophae rhamnoide seedlings and breed two hundred and seventy thousand trees per hectare ;the seedling height ,ground diameter of the seedlings after mixing have significant differences with CK (seedling height & ground diameter increase by 48 .65% & 33 .34% ,respectively than CK );the length of the main root increase by 43 .7% and its difference reach extreme significant level ;the length of the lateral root increase by 18 .8% and its difference is not significant ;root rotting rate of Hippophae rhamnoide seedlings af-ter mixing is 8 .9% and is 4 .3% lower than that of CK ;using seed coating agent is an effective measure for breeding superior seedlings in semiarid region .%采用种衣剂拌种进行沙棘育苗试验,当年秋季进行沙棘苗生长量和苗木产量的调查,探讨种衣剂拌种对沙棘种子繁苗效果的影响。结果表明:包衣剂拌种能够显著提高沙棘苗木生长量,比对照多繁育27万株灋hn-2;种衣剂拌种后繁育的苗木苗高、地径与对照差异显著,苗高比对照提高48.65%,地径提高33.34%;主根长较对照提高43.7%,差异达极显著水平;侧根数提高18.8%,差异不显著;种衣剂拌种后沙棘苗木根系腐烂率为8.9%,比对照低4.3个百分点。种衣剂拌种是半干旱区繁育沙棘优质苗木的有效技术措施。

  2. Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula

    Directory of Open Access Journals (Sweden)

    Barkat Ali Khan

    2013-12-01

    Full Text Available The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®. The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use.

  3. Pharmacokinetic properties of isorhamnetin, kaempferol and quercetin after oral gavage of total flavones of Hippophae rhamnoides L. in rats using a UPLC-MS method.

    Science.gov (United States)

    Li, Guowen; Zeng, Xiaoli; Xie, Yan; Cai, Zhenzhen; Moore, Jeffrey C; Yuan, Xiurong; Cheng, Zhihong; Ji, Guang

    2012-01-01

    An ultra performance liquid chromatography-mass spectrometric (UPLC-MS) method was developed to investigate the pharmacokinetic properties of isorhamnetin, kaempferol and quercetin from a total flavone extract of Hippophae rhamnoides L. (TFH) after single dose oral administration. Rat plasma samples were pretreated using liquid-liquid extraction, and chromatographic separation was performed on a C(18) column using a linear gradient of methanol and formic acid (0.1%). The pharmacokinetic parameters of isorhamnetin, kaempferol and quercetin from TFH in rats were quantitatively determined by UPLC with photodiode array detection (PDA). The qualitative detection of the three flavones was accomplished by selected ion monitoring in negative ion mode ESI-MS. Results of the pharmacokinetic study indicate that the three flavones in TFH were absorbed by passive diffusion in rats, and no "double-peak" phenomenon was observed in C-t curves of the three flavones from TFH except for quercetin. Results of this study indicate that the pharmacokinetic behaviors of isorhamnetin, kaempferol and quercetin when administered together in a complex herbal extract might be different than the individual behaviors of the same compounds administered in their pure forms. Results of this study also demonstrate that UPLC-MS is a rapid and practical method to determine the pharmacokinetic parameters of flavones present in an herbal extract.

  4. Effects of solid dispersion and self-emulsifying formulations on the solubility, dissolution, permeability and pharmacokinetics of isorhamnetin, quercetin and kaempferol in total flavones of Hippophae rhamnoides L.

    Science.gov (United States)

    Zhao, Guoying; Duan, Jingze; Xie, Yan; Lin, Guobei; Luo, Huilin; Li, Guowen; Yuan, Xiurong

    2013-07-01

    The aim of this study was to investigate the effects of solid dispersions (SD) and self-emulsifying (SE) formulations on the solubility and absorption properties of active components in total flavones of Hippophae rhamnoides L. (TFH). The solubility, dissolution rate, permeability and pharmacokinetics of isorhamnetin, quercetin and kaempferol in TFH SD/SE formulations and TFH were compared. The results showed that the solubility and dissolution rate of isorhamnetin, quercetin and kaempferol in SD/SE formulations were significantly enhanced compared to those in TFH, however, their intestinal permeability was comparable. The bioavailability of isorhamnetin, quercetin and kaempferol in rats remarkably increased after oral administration of TFH SD formulations compared to TFH, but there was no significant increase after oral administration of TFH SE formulations. The results of this study indicated the SD formulations on the improvement of pharmacokinetic properties of isorhamnetin, quercetin and kaempferol in TFH were much better than those of SE formulations. The improvement of pharmacokinetic properties of isorhamnetin, quercetin and kaempferol in TFH by SD formulations was probably ascribed to the enhancement of the solubility and dissolution of the three components, but was not relevant to the intestinal permeability. Therefore, as for herb extracts containing multiple components, especially for their major components with poor water solubility, solid dispersion formulations might have the better potential to enhance their bioavailability.

  5. Effect of Sea buckthorn on liver fibrosis: A clinical study

    Institute of Scientific and Technical Information of China (English)

    Ze-Li Gao; Xiao-Hong Gu; Feng-Tao Cheng; Fo-Hu Jiang

    2003-01-01

    AIM: To appraise the effect of sea buckthorn (Hippophae rhamnoides) on cirrhotic patients.METHODS: Fifty cirrhotic patients of Child-Pugh grade A and B were randomly divided into two groups: Group A as the treated group (n=30), taking orally the sea buckthom extract, 15 g 3 times a day for 6 months. Group B as the control group (n=18), taking vitamin B complex one tablet,3 times a day for 6 months. The following tests were performed before and after the treatment in both groups to determine LN, HA, collagens types Ⅲ and IV, cytokines IL6 and TNFα, liver serum albumin, total bile acid, ALT, AST and prothrombin time.RESULTS: The serum levels of TNFα, IL-6, laminin and type IV collagen in group A were significantly higher than those in the control group. After a course of sea buckthorn treatment, the serum levels of LN, HA, collagen types Ⅲand IV, total bile acid (TBA) decreased significantly as compared with those before and after treatment in the control group. The sea buckthorn notably shortened the duration for normalization of aminotransferases.CONCLUSION: Sea buckthom may be a hopeful drug for prevention and treatment of liver fibrosis.

  6. Proximate composition, mineral contents, phytochemical constituents, antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides Linn

    Directory of Open Access Journals (Sweden)

    Javid Ali

    2015-06-01

    Full Text Available Objective: To compare the proximate composition, mineral contents, antimicrobial, phytochemical and Fourier transforms infrared (FTIR spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides. Methods: Proximate composition was determined according to the described methods. Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer. Antimicrobial activities were evaluated according to the agar well diffusion method. Phytochemical qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu Japan. Results: The proximate analysis showed high content of protein and fiber in stem and bark. High content of Na (900 mg/L and K (670 mg/L was found in bark powder, while in seed, high contents of Ca (800 mg/L, Mg (725 mg/L, Fe (250 mg/L Zn (90 mg/L and Mn (65 mg/L were found compared to stem and bark. Phenols, flavonoids and tannins showed high contents in stem and bark of all extracts. The bark aqueous extract showed high zone of inhibition against Staphylococcus aureus (21 mm and Escherichia coli (20 mm, while methanol extract of stem showed high zone of inhibition (14 mm and 13 mm against Enterococcus faecalis and Escherichia coli respectively. The aqueous extract of bark documented high zone of inhibition against Aspergillus niger (21 mm and Aspergillus parasiticus (20 mm. FTIR spectra revealed the presence of OH, C-O and C=O functional groups. Conclusions: The study concludes that bark, stem and seed extracts will be useful guideline for the new syntheses of feed, food supplements and herb drugs with various combination, which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.

  7. Proximate composition, mineral contents, phytochemical constituents, antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides Linn

    Institute of Scientific and Technical Information of China (English)

    Javid Ali; Bashir Ahmad; Said Hassan; Muhammad Siddique; Farrah Gul; Shafaat Ullah

    2015-01-01

    Objective: To compare the proximate composition, mineral contents, antimicrobial, phytochemical and Fourier transforms infrared (FTIR) spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides. Methods: Proximate composition was determined according to the described methods. Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer. Antimicrobial activities were evaluated according to the agar well diffusion method. Phytochemical qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu Japan. Results:The proximate analysis showed high content of protein and fiber in stem and bark. High content of Na (900 mg/L) and K (670 mg/L) was found in bark powder, while in seed, high contents of Ca (800 mg/L), Mg (725 mg/L), Fe (250 mg/L) Zn (90 mg/L) and Mn (65 mg/L) were found compared to stem and bark. Phenols, flavonoids and tannins showed high contents in stem and bark of all extracts. The bark aqueous extract showed high zone of inhibition against Staphylococcus aureus (21 mm) and Escherichia coli (20 mm), while methanol extract of stem showed high zone of inhibition (14 mm and 13 mm) against Enterococcus faecalis and Escherichia coli respectively. The aqueous extract of bark documented high zone of inhibition against Aspergillus niger (21 mm) and Aspergillus parasiticus (20 mm). FTIR spectra revealed the presence of OH, C-O and C=O functional groups. Conclusions:The study concludes that bark, stem and seed extracts will be useful guideline for the new syntheses of feed, food supplements and herb drugs with various combination, which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.

  8. [Determination of plasma concentration of quercetin, kaempferid and isorhamnetin in Hippophae rhamnoides extract by HPLC-MS/MS and pharmacokinetics in rats].

    Science.gov (United States)

    Liu, Yu; Yang, Juan; Tuo, Yang-ling; Wei, Ting; Zeng, Yong; Wang, Ping; Meng, Xian-li

    2015-10-01

    To establish an HPLC-MS/MS method for the analysis of quercetin, kaempferid and isorhamnetin in rats plasma and study its pharmamacokinetics after an intragastrical administration of Hippophae rhamnoides extracts. Five healthy male Sprague-Dawley (SD) rats were given single doses of H. rhamnoides extracts (quercetin 26.35 mg x kg(-1), kaempferid 4.040 mg x kg(-1), isorhamnetin 31.37 mg x kg(-1)), and then their orbital sinus blood samples were collected at different time points. The drug plasma concentration of the three flavonoids was determined by HPLC-MS/MS method. After that, the main pharmacokinetics parameters were calculated by using Kinetica 5. 0. 11 software. The methodological test showed that the linear concentration ranges of quercetin, kaempferid and isorhamnetin were 7.500-600.0 μg x L(-1) (R2 = 0.998 5), 1.000-80.00 μg x L(-1) (R2 = 0.998 5 ) and 10.00-800.0 μg x L(-1) (R2 = 0.998 0), respectively. The inner and inter-days precisions were both less than 14.0%. The plasma samples showed a good stability and consistency with the requirement of biological sample analysis after the samples were frozen once and placed at - 20 degrees C for 15 d and room temperature for 6 h and the treated analytes were placed at -20 degrees C for 24 h. For quercetin, the pharmacokinetic parameter t(½β), AUC(0-∞), MRT(0.∞), C.(max) and T(max) were (113.3 ± 19.37) min, (12 542.14 ± 3 504.05) μg x h x L(-1), (119.6 ± 13.29) h, (164.6 ± 27.33) μg x L(-1) and (5.199 ± 0.840 3) h, respectively. For kaempferid, the pharmacokinetic parameters t(½β), AUC(0-t), MRT(0-∞), C(max) and T(max) were (79.85 ± 17.15) min, (934.51 ± 94.59) μg x h x L(-1), (81.50 ± 13.75) h, (80.15 ± 14.24) μg x L(-1) and (3.827 ± 0.902 7) h, respectively. For isorhamnetin, the pharmacokinetic parameters t1,2,, AUC(0-t), MRT(0-∞), C(max) and T(max) were (118.3 ± 20.73) min, (26 067.77 ± 4 124.60) μg x h x L(-1), (129.0 ± 16.30) h, (269.6 ± 29.32) μg x L(-1) and (6.513 ± 1

  9. Extraction Isorhamnetin from Marc of Hippophae rhamnoides L. with Seal Heating Method%密封加热法提取沙棘果渣中异鼠李素

    Institute of Scientific and Technical Information of China (English)

    何珺; 胡丹; 杨佳年

    2015-01-01

    采取密封加热的方法对从沙棘果渣中提取异鼠李素的工艺进行研究。采用单因素实验对提取时间、提取次数、提取温度、料液比及提取溶剂进行筛选,之后对提取条件进行正交验证实验。实验确定了密封加热法提取沙棘果渣中异鼠李素的最佳工艺为:55℃条件下用V 乙酸乙酯∶V95%乙醇=4∶6的提取液水浴加热2次,每次3 h,料液比为1∶20(g∶mL)。%Extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were studied. Single factor experiment was used to select extraction time, extraction times, extraction temperature, solid-liquid ratio and extraction solvent. Then the orthogonal verification experiment was used. The best processes of extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were as follows:under temperature 55℃, Vethyl acetate∶V95%ethyl alcohol were 4∶6 as extraction solvent, extraction time 3 h, material to liquid ratio 1∶20(g∶mL), and extraction times 2.

  10. Morphological and physiological response ofHippophae rhamnoides on alkaline salt stress%沙棘对碱性盐胁迫的形态和生理响应

    Institute of Scientific and Technical Information of China (English)

    于畅; 王竞红; 薛菲; 江远芳

    2014-01-01

    采用50、100、150 mmol·L-13种浓度的Na2CO3、NaHCO3及二者混合盐对2年生沙棘幼苗进行胁迫处理,通过比较分析胁迫后沙棘幼苗的形态和细胞膜透性、脯氨酸含量、可溶性糖含量的变化,探讨沙棘对Na2CO3、NaHCO3和混合盐胁迫的适应能力。结果表明:在胁迫条件下,沙棘出现枯黄、落叶等盐害症状,随着胁迫浓度的加大和时间的延长,症状表现逐渐加重;电导率、脯氨酸含量、可溶性糖含量均随胁迫程度的加深呈上升趋势,胁迫前期变化相对稳定,后期变化幅度较大;形态表现和生理指标实验均反映出,不同类型盐碱胁迫对沙棘的伤害程度不同,单盐的迫害作用大于复盐,碱性盐的伤害作用大于酸性盐,即Na2CO3>NaHCO3>混合盐碱。此结果对提高沙棘在东北地区护坡工程中的应用提供了技术支持。%The two-year-oldHippophae rhamnoides seedlings were treated by stress method with three kinds of salts (Na2CO3, NaHCO3, the mixture of Na2CO3 and NaHCO3) under different concentrations (50,100,150 mmol/L). The changes of morphology, cell membrane permeability, proline content and soluble sugar content ofH. rhamnoides seedlings after being stress treatment were compared and analyzed. Thus, the adaptive capacity ofH. rhamnoides seedlings under the stress of Na2CO3, NaHCO3 and the mixture of Na2CO3 and NaHCO3 was studied. The results show that under the condition of stress, the salt damage symptoms toH. rhamnoides seedlings appeared, such as leaf blight-yellow and leaf fell, with the increase of stress concentration and time, the symptoms gradually worsened; the electrical conductivity, proline content, soluble sugar content increased with the trend of stress degree going upward, in the stress earlier stage the change was relatively stable, later changed greatly; the morphology and physiological indicators reflected that the damage degrees of saline alkali stress to

  11. 沙棘AM真菌孢子形态结构及其生态适应性%Morphological Structure and Ecological Adaptability of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Hippophae rhamnoides

    Institute of Scientific and Technical Information of China (English)

    贺学礼; 杨欢; 杨莹莹; 郭辉娟; 韦阅

    2013-01-01

    为了探明干旱环境下AM真菌形态结构及其生态适应性,2009年7月在内蒙古和河北农牧交错带选取4个样地,采集沙棘(Hippophae rhamnoidesL.)根围土壤样品,利用光学显微镜和扫描电镜对分离的AM真菌进行分类鉴定.共分离鉴定3属17种AM真菌,其中球囊霉属(Glomus)11种,无梗囊霉属(Acaulospora)5种,盾巨孢囊霉属(Scutellospora)1种.沙棘AM真菌孢子具有体积小、颜色深、孢壁厚,整体形态不饱满等特征,表现出对干旱荒漠环境的生态适应性.扫描电镜下,不同属种孢子表面纹饰特征差异明显,可作为AM真菌属种分类的参考依据.%In order to elucidate the morphological structure and ecological adaptability of arbuscular mycorrhizal fungi in arid environment, some soil samples were collected from the rhizosphere of Hippophae rhamnoides L. At four sapling sites in a farming-pastoral ecotone in a region between Inner Mongolia and Hebei Province in July 2009. Seventeen AM fungal species in three genera were isolated and identified with optical microscope and scan e-lectricity microscope. In which 11 species were in Glomus, 5 in Acaulospora and 1 was in Scutellospora. The results indicated that AM fungal spore had the obvious ecological adaptability, and the spore was characterized by its small shape, deep color, thick wall and un-full overall shape. SEM showed that fungal spore morphology was different a-mong the 17 species, especially in surface veins, which can be used as a reference index for AM fungal identification.

  12. Density and Biomass Dynamics of Hippophae rhamnoides L.subsp.sinensis Population in Mu Us Sandland%毛乌素沙地中国沙棘种群数量动态研究

    Institute of Scientific and Technical Information of China (English)

    李根前; 赵粉侠; 李秀寨; 韦宇

    2004-01-01

    Based on the measurement of all individuals of population at different successional stage and following excavation of clones, the density and biomass dynamics of Hippophae rhamnoides L. subsp, sinensis population in Mu Us sandland were analyzed with the methods of plots arranged with age and reverse age class addition(RAA). The main results were as follows:(1)The density of clone population increased with the population growth before 5-year-old. The population biomass accumulating also increased by Logistic equation in the period of age structure of population developed from increasing type to mid-decreasing type.Then they tended to be decrease. While the population density, population biomass augmented again with the emergence of gap regeneration, and the dominant status of H.rhamnoides L.subsp, sinensis population and the stability of the community were maintained. (2)The decrease process of density of daughter ramets population lagged behind that of mother ramets population,and the numerical ratio of daughter ramets population within the population gradually enlarged. As the population thinned, the composition of population changed from more clones with less daughter ramets to less clones with more daughter ramets and the genetic diversity of population reduced. (3)The prosperous stage of biomass accumulation appeared from 8-year-old to 16-year-old and its peak appeared at 12-year-old. And the relationship between the individual mean weight and population density could be expressed by the law of power-3/2 in the period of age structure of population developed from stable type to decreasing type.

  13. Three-dimensional Shade Seedling Nursing Method of Hippophae Rhamnoides L. Suitable for Arid Desert Area in Xinjiang Autonomous Region%适用于新疆干旱荒漠区的沙棘立体遮荫育苗法

    Institute of Scientific and Technical Information of China (English)

    王东健; 陈奇凌; 庄振刚; 张献辉; 花东来

    2014-01-01

    In particularly bad area with very adverse circumstances in acrid desert region, it is needed to afforest by culturing seedling plant of Hippophae rhamnoides L. with strong stress resistance and good economic benefit. The study showed that 10~15 days before the seabuckthorn sowing planting the early-maturing maize by the side of seabuckthorn rows for the three-dimensional shade seedling nursing with windbreak effect can not only improve the rate of emergence and seedling survival of Hippophae rhamnoides L., but also meet Hippophae rhamnoides L.’ need for strong illumination after the harvest of maize. Compared with regular field seeding and greenhouse nursery, this practice is characterized by high rate of emergence, high growth (about 14cm more than normal nursery), less initial input and high final comprehensive gains (about 3113 yuan increased per mu). So the three-dimensional shade nursing method serves as an effective measure for Hippophae rhamnoides L. seeding and seedling nursing in ar-id desert area.%在新疆干旱荒漠区的环境特别恶劣地段,需要培育抗逆性强且经济效益较好的沙棘实生苗造林。经试验研究,在沙棘播种前10~15d于沙棘行边稀疏地播种早熟玉米,进行沙棘立体遮荫挡风育苗,既能提高沙棘出苗率和保苗量,又能在后期玉米成熟砍去后,满足沙棘对强光照的需要。这一方法比起大田常规育苗和固定温棚育苗,出苗率高,苗高较常规育苗提高14cm ,且前期投入少,后期综合收益大,亩效益较常规育苗提高3113元。立体遮荫育苗法,是适用于新疆干旱荒漠区沙棘播种育苗的有效措施。

  14. Purification and characterization of water soluble polysaccharide JS1 from Hippophae rhamnoides%沙棘果水溶多糖JS1的分离鉴定

    Institute of Scientific and Technical Information of China (English)

    王桂云; 梁忠岩; 张丽萍; 苗春艳

    1999-01-01

    OBJECTIVE:To study the purification and characterization of water soluble polysaccharide JS1 from Hippophae rahamnoides L.fruits that had been extracted with water.METHODS:JS1 was purified by gel chromatography on DEAE-Sep-hadex A-25.its homogeneity was proved by gel chromatogrophy on Sepharose CL-4B glass fiber paper electrophoresis and specific rotator power.Its stral composition was studied by means of GC,IR,etc.RESULTS:JS1 is a water solube polysaccharide.It is consisted of Ara,Xyl,Gal,Glc with the molarity rate of 1∶6∶12∶4 in turn by means of GC.CONCLUSION:JS1 is a neutral heteropolysaccharide.%目的:从已提取过水溶性多糖后的沙棘果残渣中,进一步用稀碱液(0.1mol.L-1NaOH溶液)提取多糖JS1,并研究其组成性质。方法:利用DEAE-Sephadex A-25柱层析法纯化沙棘果碱提水溶多糖,并采用玻璃纤维纸电泳,Sepharos CL-4B柱层析比旋度等方法证明组分的均一性,同时用气相色谱、红外光谱等方法对其组成进行研究。结果:分离得到JS1水溶性多糖,经气相色谱分析得单糖组分为Ara,Xyl,Gal,Glc,摩尔比依次是1∶6∶12∶4。结论:JS1为中性杂多糖。

  15. High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn

    Science.gov (United States)

    Zhao, Lu; Wen, E; Upur, Halmuart; Tian, Shuge

    2017-01-01

    Context: Sea buckthorn (Hippophae rhamnoides L.) as a traditional Chinese medicinal plant has various uses in Xinjiang. Objective: A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. Settings and design: Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. Materials and Methods: The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. Statistical performances: The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (R2 > 0.9997). Results: The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang. Conclusions: The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn. SUMMARY Quantitative analysis method of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the extract of sea buckthorn pulp and seed is developed by high-performance liquid chromatography (HPLC) diode array detection.This method is simple and accurate; has strong specificity, good precision, and high recovery rate; and provides a reliable basis for further development of the substances in the pulp and seed of sea buckthorn.The method is widely used for content determination of active ingredients or physiologically

  16. Influence of hippophae rhamnoides on neuropeptide Y,gastric emptying and body fat in children with func-tional dyspepsia%沙棘对功能性消化不良患儿神经肽 Y、胃动力及体脂的影响

    Institute of Scientific and Technical Information of China (English)

    肖满田; 丘小汕; 蔡泳仪

    2014-01-01

    目的:探讨沙棘对功能性消化不良( FD)患儿食欲因子神经肽Y( NPY)水平及其胃动力、体脂的影响。方法选择符合FD诊断标准的患儿120例,采用单纯随机抽样方法分为三组,Ⅰ组单纯予以沙棘干乳剂治疗,Ⅱ组予以多潘立酮治疗,Ⅲ组予以沙棘干乳剂加多潘立酮治疗,共8周,治疗前后分别测血浆NPY水平,并采用B超检查作胃液体排空试验测餐后30 min、60 min、90 min和120 min的胃窦残留率,测量患儿皮下脂肪平均值、体脂比、上臂围、体质量、身高。结果Ⅰ组、Ⅱ组、Ⅲ组血浆食欲因子NPY水平治疗前分别是(107.52±24.12)ng/L、(114.63±17.95)ng/L和(114.56±25.10)ng/L,差异无统计学意义(F=1.307, P>0.05);经治疗后,三组血浆NPY分别是(137.38±29.86) ng/L、(121.43±13.78) ng/L和(149.27±31.9) ng/L,差异有统计学意义(F=11.23,P<0.05);Ⅰ组、Ⅲ组NPY水平治疗后明显高于治疗前(t=4.95、5.40,均P<0.05)。Ⅰ组、Ⅲ组餐后60 min、90 min和120 min的胃窦残留率比Ⅱ组同期均明显减少( F=4.93、6.40、4.61,P<0.05);Ⅰ组、Ⅲ组皮下脂肪平均值﹑体脂比、上臂围、体质量增加值显著高于Ⅱ组( F=16.80、46.9、21.06、12.08,均P<0.05)。而Ⅰ组与Ⅲ组两组治疗后食欲因子NPY水平、胃窦残留率及皮下脂肪平均值﹑体脂比、上臂围、体质量比较,差异均无统计学意义(均P<0.05)。结论沙棘对FD患儿食欲因子NPY有明显调节作用,促进胃排空,同时患儿体脂明显增加,体格发育明显改善。%Objective To explore the effect of hippophae rhamnoides on the level of plasma appetite factors neuropeptide Y ,the gastric motility and on their body fat in children with functional dyspepsia .Methods A hundred and twenty children with functional dyspepsia were

  17. 黄土高原沙棘根际氢氧化细菌的分离与种属分布%The solation, identification and phylogeny of hydrogen-oxidizing bacteria from Hippophae rhamnoides rhizosphere in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    曹桂林; 王卫卫; 刘慧芬; 唐明

    2012-01-01

    Aim To study hydrogen oxidizing bacteria's genera from Hippophae rhamnoides rhizosphere in the Loess Plateau in Shaanxi Province of China. Methods Gas-cycle incubation system was used to isolate bacteria strains. The strains containing hydrogenase activity were selected by TTC (triphenyltetrazo-lium chloride) test and hedrogen-oxidizing ability of the strains. These strains are identified based on their morphological, physiological and biochemical characteristics. 16S rRNA gene sequence analysis is used to built the phylogenic tree of the strain which has strongest hedrogen-oxidizing ability. Results Six strains were identified as hydrogen oxidizing bacteria initially and identified as members of genera Bacillus, Aeromonas, Pseudomonas and Micrococcus, respectively. The 16S rRNA gene sequence analysis suggested that the strain FS2 (GenBank accession number GU084156) was clustered together with Bacillus in phylogenetic tree, with the sequence similarity more than 99%. Conclusion The experiments show that hydrogen-oxidizing bacteria distribute widely in nature, providing base material to analyze the structure of population of hydrogen-oxidizing bacteria.%目的 研究陕西黄土高原沙棘(Hippophae rhamnoides)根际氢氧化细菌种属分布.方法 利用持续通H2的气体循环培养体系分离纯化细菌.通过TIC(2,3,5-氯化三苯基四氮唑)试验和氧化H2能力测定筛选含有氢化酶的菌株.根据其培养特征、形态特征和生理生化特征进行菌株鉴定.用16S rRNA基因序列分析法对氧化氢能力最强的优势菌株构建系统发育树.结果 筛选出6株菌初步确定为氢氧化细菌,并划分为4个属:芽孢杆菌属(Bacillus)、气单胞菌属(Aeromonas)、假单胞菌属(Pseudomonas)和微球菌属(Micrococcus).其中菌株FS2的16S rRNA基因序列(Gen-Bank登录号为GU084156)与芽孢杆菌属相似性为99%,在系统发育树上位于同一分支,因此将菌株FS2归为芽孢杆菌属(Bacillus).结论 说

  18. Simultaneous determination of quercetin, kaempferol and isorhamnetin in Hippophae rhamnoides L.fruit by HPLC%高效液相色谱法同时测定沙棘果中槲皮素、山柰酚和异鼠李素的含量

    Institute of Scientific and Technical Information of China (English)

    樊鑫梅; 申雪丽; 闫丽丽; 王新春; 尹俊涛

    2012-01-01

    OBJECTIVE To develop an HPLC method for simultaneous determination of three flavonoids such as quercetin, kaempferol and isorhamnetin in Hippophae rhamnoides L. fruit. METHODS The column was Kromasil-C18 (250 mm × 4. 6 mm, 5 βm), the mobile phase was acetomtrile(A)-0. 6% phosphoric acid solution(B) with linear gradient elution(0- 15 min, 33%→32% A; 15 - 30 min,32%→31% A) , the flow rate was 1. 0 mL-min-1 , the column temperature was 30 °C and the detection wavelength was 370 ran, RESULTS The method had good linear relationship within the range of 1. 68 - 26. 88 fig· mL-1 for quercetin, 1. 52 - 24. 32βg·mL~1 for kaempferol, and 1. 76-28. 16 ·g-mL-1 for isorhamnetin. The average recoveries of quercetin, kaempferol and isorhamnetin were 98. 55% , 97. 63% and 97. 98% , respectively and the RSD were 1. 48% , 1. 66% and 1. 54%, respectively. CONCLUSION This method is simple, accurate and reliable. It is can be used to determine the contents of quercetin, kaempferol and isorhamnetin in Hippophae rhamnoides L. fruit,%目的:采用高效液相色谱法同时测定沙棘果中槲皮素、山柰酚和异鼠李素的含量.方法:Kromasil-C18 (250 mm×4.6mm,5 μm)色谱柱,流动相为乙腈(A)-0.6%磷酸(B)水溶液,线性梯度洗脱(0~15 min,33%→32%A;15~30 min,32%→31%A),流速1.0 mL·min-1,柱温30℃,检测波长370nm.结果:槲皮素,山柰酚和异鼠李素的质量浓度分别在1.68~26.88,1.52~24.32和1.76~28.16 μg·mL-1范围内线性关系良好,平均回收率分别为98.55%,97.63%0和97.98%,RSD分别为1.48%,1.66%和1.54%.结论:所建立的方法简单易行,准确可靠,可用于沙棘果中槲皮素、山柰酚和异鼠李素的含量测定.

  19. 沙棘叶提取物抗登革病毒的实验研究%Effect of Hippophae rhamnoides Leaf Extract Against Dengue Virus Infection in Human Blood-derived Macrophages

    Institute of Scientific and Technical Information of China (English)

    花圣卓; 李铂岩

    2012-01-01

    Dengue virus occurs as four distinct serotypes,called Dengue 1,2,3,and 4.Symptomaticdengue virus infection ranges from a self limited febrile illness,dengue fever(DF),to a more severedisease,dengue hemorrhagic fever/dengue shock syndrome(DHF/DSS).The anti-Dengue treatmentis severely hampered as no specific therapeutic agents are available.Even present treatmentstrategies for Dengue are more supportive than curative.In the present study anti-dengue activity of Hippoplzae rhamnoides{Seabuckthorn,SBT)leaf extract 4vas evaluated in Dengue virus type-2 infected blood-derived human macrophages as macrophages are the primary target of Denguevirus infection.Infected cells were treated with SBT leaf extract and compared with commerciallyavailable anti-viral drug,Ribavirin.The extract was able to maintain the cell viability of Dengue-infected cells at par with Ribavirin along with the decrease and increase in TNF-[alpha]and IFN-[gamma]respectively.Anti-dengue activity of SBT extract was further determined by the traditionalplaque assay.These observations suggest that the SBT leaf extract has a significant anti-dengueactivity and has the potential for the treatment of Dengue.%登革病毒依抗原性不同,可分为1、2、3、4四个血清型。它除了能导致一定范围内发热的典型登革热外,还能引致更严重的登革出血热和登革休克综合症。由于没有具体有效的治疗药物,目前尚无好的治疗登革热的方法,治疗方案多是支持性的。本实验评估了在2型登革病毒感染的人血源性巨噬细胞中沙棘叶提取物的抗登革病毒活性,选择巨噬细胞的原因是因为巨噬细胞是登革病毒感染的首要目标。受感染的细胞用沙棘叶提取物治疗并与市售的抗病毒药物利巴韦林作比较,研究发现沙棘叶提取物维持登革病毒感染细胞活力的能力几乎等同于利巴韦林。传统的空斑试验进一步确定了沙棘叶提取物的抗登革病毒活性。

  20. Influence of Total Flavones of Hippophae Rhamnoides L on proliferation and cell circles of HUVEC%沙棘总黄酮对人脐静脉血管内皮损伤细胞增殖及细胞周期的影响

    Institute of Scientific and Technical Information of China (English)

    苑博; 张放; 程嘉艺; 康廷国

    2011-01-01

    Objective To investigate the protective effect of the total flavones of Hippophae Rhamnoides L(TFH) on damaged human umhilical vein endothelial cells (CRL-1730). Methods Injured model of CRL-1730 were induced by H2O2 ,and randomly divided into fourgroups ,the normal group were not given any intervention ;the model group was added 250 μmol/L H2O2 ;the TFH groups were alded TFH with the fina1 concentrations of 50,100,200 μg/ml respectively, and added H2O2(dose ditto) 24 hs after training;the Danshen group was added Danshen injection with the final concentration of 2.5 mg/ml, and added H2O2 ( dose ditto ) 24 hs alter training. Cells were intervened for 4 ha and then determined by MTT colorimetric to detect cell viability,the percentage of cell circles were detected by flow cytometry. Results Conpared with the normad group, the cell viability and the percentage d S phase cells decreased,and the percentage d G2/M phase cells increased in the model group;comparel with the model group, the cell viability and the percentage d S phase cells,G2/M phase cells in the TFH group and Danshen group increased, and the percentage of G0/G1 phase cells decreased. Conclusions The TFH has precise protective effect on damag ed HUVECs, it can raise cell proliferation activity and increase ratio d S phase cells.%目的 探讨沙棘总黄酮对损伤人脐静脉血管内皮细胞株(CRL-1730)的保护作用.方法 采用H2O2诱导CRL-1730细胞损伤模型,并将细胞随机分为四组,正常组不予任何干预;模型组加入250 μmol/L H2O2;沙棘组加入沙棘总黄酮终浓度分别为50、100、200 μg/ml,继续培养24 h后加入H2O2(剂量同上);丹参组加入丹参注射液终浓度2.5 mg/ml,继续培养24 h后加入H2O2(剂量同上).细胞干预4 h后用MTT比色法检测各组细胞活性,并用流式细胞仪测定各组细胞周期.结果 与正常组比较,模型组细胞增殖活性下降,S期细胞比率降低,G2/M期细胞比率增加.与模型组比较,沙棘组

  1. Formulation and evaluation of antisebum secretion effects of sea buckthorn w/o emulsion

    Directory of Open Access Journals (Sweden)

    Naveed Akhtar

    2010-01-01

    Full Text Available Purpose : This study was designed to formulate and evaluate the anti-sebum secretion effects of a topical skin-care cream (w/o emulsion of sea buckthorn versus its vehicle (Base as control. Materials and Methods : Concentrated sea buckthorn (H.rhamnoides fruit extract was entrapped in the inner aqueous phase of w/o emulsion. Base containing no extract and a Formulation containing 1% concentrated extract of H.rhamnoides was formulated. Lemon oil was incorporated to the odor. Both the Base and the Formulation were stored at different storage conditions for a period of 4 weeks to predict their stability. Different stability parameters i.e.; physical stability, centrifugation, and pH were monitored at different time intervals. Both the Base and the Formulation were applied to the cheeks of 10 healthy human volunteers (n=10 for a period of 8 weeks. Result : The expected organoleptic stability of creams was achieved from 4 weeks in-vitro study period. Odor disappeared with the passage of time due to volatilization of lemon oil. The pH of the Formulation showed significant ( P = 0.0002 decline due to high concentration of organic acids present in sea buckthorn. Similarly the Formulation showed statistically significant ( P < 0.05 effects on skin sebum secretion. Conclusion : The in vitro results showed a good stability over 4 weeks of observation period of both the Base and Formulation and the Formulation has anti sebum secretion effects over 8 weeks of observation period.

  2. 母株年龄和生长调节剂对俄罗斯大果沙棘嫩枝扦插生根的影响%Effects of donor tree ages and plant growth regulators on the softwood cutting propagation of Hippophae rhamnoides

    Institute of Scientific and Technical Information of China (English)

    杨立学; 王海南; 范晶

    2011-01-01

    ×concentration and age×plant growth regulator concentration were highly significant for percent rate,root number and average root length.The interactive effects of donor tree age×the concentration of plant growth regulators×plant growth regulators were highly significant for rooting rate of H.rhamnoides.

  3. Wound healing potential of a polyvinyl alcohol-blended pectin hydrogel containing Hippophae rahmnoides L. extract in a rat model.

    Science.gov (United States)

    Kim, Jin; Lee, Chang-Moon

    2017-06-01

    In this study, we investigated the effect of a polyvinyl alcohol-blended pectin hydrogel (PVA-PT HG) containing the extracts of Hippophae rhamnoides L. (H. rhamnoides L.) leaves on wound healing in a rat model. The total phenolic content in the extract solution was 40.64±2.7 GAE mg/g and that of flavonoids was 13.15±1.8 QE mg/g. Of the total flavonoids in HGs, 61.6 and 50.0% were released at pH 5.5 and 7.4 after 60min. In rat acute wound models, the wound size was reduced significantly and the recovery rate was significantly higher after treatment with HG containing the extracts, compared with treatment with the control and HG only. The wound healing effects of the HG containing the extracts were confirmed by histological evaluation of the wound tissue. Therefore, HG containing extracts from H. rhamnoides L. leaves enhanced wound healing effectively, and so may be developed as a cover to promote wound healing.

  4. Attributes of Seabuckthorn (Hippophae rhamnoides L. to Meet Nutritional Requirements in High Altitude.

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    Tsering Stobdan

    2010-03-01

    Full Text Available The diet of humans living in different geographical and climatic regions of the earth varies greatly in both quantity and composition of foods. Evidence is accumulating that indicates that there is a high risk of malnutrition at high altitude because of the usual lack of fresh food and environmental factors. Lack of nutritious diet in the difficult terrain is a potential stressor that elicits oxidative stress. The excretion of minerals from the body is higher in high altitude condition. The altered nutritional requirement can be met to a large extend by regular consumption of locally grown fruits and vegetables. Results of analysis of Seabuckthorn growing in Leh valley of Trans-Himalaya showed the presence of high content of multivitamins including vitamin C (275 mg/100g, vitamin A (432.4 IU/100g, vitamin E (3.54 mg/100g, Riboflavin (1.45 mg/100g, Niacin (68.4 mg/100g, Pantothenic acid (0.85 mcg/100g, vitamin B-6 (1.12 mg/100g, and vitamin B-2 (5.4 mcg/100g. Similarly, mineral elements composition revealed high amount of minerals including potassium (647.2 mg/l, calcium (176.6 mg/l, iron (30.9 mg/l, magnesium (22.5 mg/l, phosphorous (84.2 mg/l, sodium (414.2 mg/l, zinc (1.4 mg/l, copper (0.7 mg/l, manganese (1.06 mg/l and selenium (0.53 mg/l.Defence Science Journal, 2010, 60(2, pp.226-230, DOI:http://dx.doi.org/10.14429/dsj.60.344

  5. Acute and sub acute toxicity and efficacy studies of Hippophae rhamnoides based herbal antioxidant supplement

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    Rashid Ali

    2012-01-01

    Conclusion: The data obtained indicate no toxicity of this antioxidant supplement up to the highest dose studied. Efficacy in terms of increased bioavailability of vitamin A and C in human volunteers indicates the clinical usefulness of the supplement.

  6. In silico -- based combinatorial pharmacophore modelling and docking studies of GSK-3 and GK inhibitors of Hippophae

    Indian Academy of Sciences (India)

    Sushil Kumar Middha; Arvind Kumar Goyal; Syed Faizan; Nethramurthy Sanghmitra; Bharat Chandra Asistha; Talambedu Usha

    2013-11-01

    Type 2 diabetes is an inevitably progressive disease, with irreversible cell failure. Glycogen synthase kinase and Glukokinase, two important enzymes with diverse biological actions in carbohydrate metabolism, are promising targets for developing novel antidiabetic drugs. A combinatorial structure-based molecular docking and pharmacophore modelling study was performed with the compounds of Hippophae salicifolia and H. rhamnoides as inhibitors. Docking with Discovery Studio 3.5 revealed that two compounds from H. salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 receptor and play a role in its inhibition; whereas in the case of Glucokinase, only one compound from both the plants, i.e. vitamin C, had good binding characteristics capable of activation. The results help to understand the type of interactions that occur between the ligands and the receptors. Toxicity predictions revealed that none of the compounds had hepatotoxic effects and had good absorption as well as solubility characteristics. The compounds did not possess plasma protein-binding, crossing blood–brain barrier ability. Further, in vivo and in vitro studies need to be performed to prove that these compounds can be used effectively as antidiabetic drugs.

  7. In silico-based combinatorial pharmacophore modelling and docking studies of GSK-3β and GK inhibitors of Hippophae.

    Science.gov (United States)

    Middha, Sushil Kumar; Goyal, Arvind Kumar; Faizan, Syed Ahmed; Sanghamitra, Nethramurthy; Basistha, Bharat Chandra; Usha, Talambedu

    2013-11-01

    Type 2 diabetes is an inevitably progressive disease, with irreversible beta cell failure. Glycogen synthase kinase and Glukokinase, two important enzymes with diverse biological actions in carbohydrate metabolism, are promising targets for developing novel antidiabetic drugs. A combinatorial structure-based molecular docking and pharmacophore modelling study was performed with the compounds of Hippophae salicifolia and H. rhamnoides as inhibitors. Docking with Discovery Studio 3.5 revealed that two compounds from H. salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 beta receptor and play a role in its inhibition; whereas in the case of Glucokinase, only one compound from both the plants, i.e. vitamin C, had good binding characteristics capable of activation. The results help to understand the type of interactions that occur between the ligands and the receptors. Toxicity predictions revealed that none of the compounds had hepatotoxic effects and had good absorption as well as solubility characteristics. The compounds did not possess plasma protein-binding, crossing blood-brain barrier ability. Further, in vivo and in vitro studies need to be performed to prove that these compounds can be used effectively as antidiabetic drugs.

  8. SPECTROSCOPIC STUDY OF SEA BUCKTHORN EXTRACTS

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    Carmen Mihaela Topală

    2014-11-01

    Full Text Available The application of sea buckthorn oil is to incorporate the oil into foodstuffs such as milk, yoghurt, cheese, butter, juice and snacks which represents new opportunities for food manufacturers, food supplements and nutraceuticals providing nutritional supports. The FTIR spectroscopy is a powerful technique for assessing food production and studied materials provides fundamental information on the behavior of the spectral metabolites and bio product. The extracts were studied from two varieties of sea buckthorn oil Pitesti I and II. Oil obtained from peel and seeds by the Soxhlet extraction with hexane solvent and CO2 supercriticalwas analyzed by FTIR spectroscopy. The concentration of fatty acids in oil extracted from seeds and peels was similar in both extraction techniques.

  9. USE OF SEA BUCKTHORN FRUITS IN THE PASTRY MANUFACTURING

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    RODICA A. STURZA

    2016-04-01

    Full Text Available Different researches on optimizing the shelf life of pastries (gingerbread, sponge cakes that are produced by adding sea buckthorn flour (2 - 4 % by weight of the flour used are presented in this study. This study shows the impact of biologically active substances on structural and mechanical, physicochemical, microbiological properties as well as the antioxidant activity of products under the conditions of in vitro gastric digestion. It has been demonstrated that the sea buckthorn flour increases the porosity of pastries, reduces the wet gluten amount and this contributes to moisture loss. The organoleptic assessment indicates that the addition of 2 % sea buckthorn flour improves the appearance, the color and the consistency of pastries. Microbiological analysis showed that samples with added sea buckthorn flour exhibit microbiological stability due to the sea buckthorn chemical composition. The antiradical activity DPPH• in conditions of in vitro gastric digestion of the samples with added sea buckthorn flour increases in a positive way, indicating a clearly positive effect on health.

  10. Assessment of antimycotic activity of seabuckthorn (Hippophae rhamnoides leaf exacts against common fungi associated with skin dermatitis

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    Harshit Verma

    2013-08-01

    Full Text Available Aim: To study the antimycotic activity of seabuckthorn leaf extracts against common fungi associated with skin infection. Materials and Methods: A total of 31 isolates were collected from the skin cases of animals and the leaves of seabuckthorn collected from the Lahaul and Spiti valley of the Himachal Pradesh. The extracts from the leaves were extracted according to standard procedures. Antimycotic activity was examined by employing agar disc diffusion method on Roswell Park Memorial Institute medium (RPMI. Different concentrations of leaf extracts i.e. 0.50%, 2%, 3%, 4%, 5%, respectively were used. Results: The study revealed that the inhibitory effect of SBT leaf extract at 5% concentration was observed to be almost 80% in 12 hrs of reading as compared to the standard drugs used as positive control against fungal isolates. Conclusion: Plants are important source of potentially useful structures for the development of new chemotherapeutic agents. The first step towards this goal is the in vitro antimycotic activity assay. In the present study, a wide range of pathogenic microorganisms were recovered from the clinical samples, including bacteria and fungus. The seabuckthorn methanolic leaf extracts (hot and cold were studied for their antimycotic activity against common fungal pathogens. [Vet World 2013; 6(4.000: 205-208

  11. Whitening efficacy of plant extracts including Hippophae rhamnoides and Cassia fistula extracts on the skin of Asian patients with melasma

    Science.gov (United States)

    Akhtar, Naveed; Hussain, Irshad; Abbas, Khwaja Asad; Rasul, Akhtar

    2013-01-01

    Introduction Melasma/hyperpigmentation and solar damage of the skin remains a difficult problem to treat. Various types of whitening agents are used to treat hyperpigmentation. A change has been observed recently to use plant extracts as skin whitening agents. Aim To compare the effectiveness of emulsion formulations containing plant extracts that include catechins/polyphenols and placebo without plant extracts, on patients with melasma. Material and methods Two groups of 25 patients each (aged 21–35 years), who reported to the outpatient department of BV Hospital and Personal clinic of a dermatologist, were included in the study. Volunteers applied the formulations with plant extracts and placebo to one side of the cheek. Prior to the study, signed consent was obtained from each patient. The tyrosinase inhibitory activity of the extracts and formulations was tested in vitro. The pigment density of patients was evaluated biometrologically using Mexameter® and subjectively using a visual survey before and after treatment of 12 weeks. The approval of the Institutional Ethics Committee of Faculty of Pharmacy, the Islamia University of Bahawalpur was obtained before the study. One-way ANOVA and Kruskal-Wallis tests were used in the statistical analysis. Results A significant decrease in the level of melanin was determined in all 50 patients who used a plant extract containing catechin (p ≤ 0.05). The difference between pre- and post-treatment levels of melanin was statistically significant (p = 0.05). Formulations prepared with plant extracts containing catechin were found effective on melasma, compared to the placebo. Conclusions Formulations containing plant extracts that are not yet being used widespread commercially on melasma could be an effective alternative treatment of melasma. PMID:24278079

  12. The Effects of Total Flavones of Hippophae Rhamnoides L. on Cardiac function and Hemodynamics of Normal Subjects

    Institute of Scientific and Technical Information of China (English)

    冯养正; LI Yi; LIAO Zhao-jun

    2006-01-01

    Healthy volunteers were randomly divided into TFH group(22 subjects) and placebo control group (20 subjects). We used double-blind method. The cardiac performance and hemodynamics of the subjects before and after administration of TFH or placebo were determined by Admittance and STI methods, Oral TFH in a dose of 10 mg could significantly decrease the PEP/LVET, ICT/LVET and ICT of the mormal subjects(P<0.05). Oral TFH could increase SV(P<0.05). CO, CI, SWI, CWI and LI also tended to increase. Oral TFH also markedly reduced TPR (P<0.05) and increasedc (P<0.05). After administration of TFH, HR and HP did not significantly change(P> 0.05). After administration of placebo, the parameters observed did not significantly change(P>0.05). The above results show that TFH can strengthen myocardial contractility and pump function of the heart, reduce total peripheral vascular resistance and increase vascular clasticity in normal subjects.

  13. Pre-logging Treatment of Invasive Glossy Buckthorn (Frangula alnus Mill. Promotes Regeneration of Eastern White Pine (Pinus strobus L.

    Directory of Open Access Journals (Sweden)

    Thomas D. Lee

    2016-12-01

    Full Text Available Non-native glossy buckthorn (Frangula alnus Mill. is invasive in forests of the northeastern USA but little is known of its effects on tree regeneration. We tested whether killing buckthorn stems before logging reduces its post-logging abundance and increases the density and height of eastern white pine (Pinus strobus L. seedlings. Three 0.4 ha plots were clearcut, three were thinned, and three were left as controls. Each plot had previously been divided into three subplots that received different buckthorn treatments during the two years before logging. Buckthorn treatments were (1 stems cut at base five times; (2 stems cut once then heat killed four times; (3 untreated control. Three years post-logging, buckthorn density and stem height were unaffected by logging but equally reduced by the two buckthorn treatments. Buckthorn reduction increased density and height of pine seedlings, and seedling height also increased with logging. In the fifth year post-logging, pine height growth and biomass were greater in clearcut than in thinned treatments, greater in areas of buckthorn removal and, within treated subplots, greater in areas with low buckthorn density than in thickets of recovering buckthorn. Thus, although buckthorn inhibited regenerating pine, pre-logging destruction of buckthorn stems reduced such competition for at least four years.

  14. Antioxidant properties of isolated isorhamnetin from the sea buckthorn marc.

    Science.gov (United States)

    Pengfei, Liu; Tiansheng, Deng; Xianglin, Hou; Jianguo, Wang

    2009-06-01

    In the present study, the process of separation and purification of isohamnetin from marc of sea buckthorn was obtained. The antioxidant properties of the pure isolated isorhamnetin were evaluated by the scavenging of the diphenylpicrylhydrazyl radical (DPPH), iron (III) to iron (II)-reducing, and iron-chelating assays. High purity isorhamnetin (92.1%) was obtained and the results of antioxidant assays showed that isorhamnetin performed significantly compared with ascorbic acid and BHT, and the linear correlations were good in these assays. In conclusion, isorhamnetin may have potential as a natural antioxidant to alternate synthetic substances as food additive with its antioxidant activity.

  15. Phytochemical analysis, antioxidant and antibacterial effects of sea buckthorn berries.

    Science.gov (United States)

    Chaman, Saadia; Syed, Nawazish-I-Husain; Danish, Zeeshan; Khan, Farrakh Zia

    2011-07-01

    Sea buckthorn berries are therapeutically used as folk medicine for a variety of diseases, however, the scientific evidence is hardly available to support their role. This study explored their chemical constituents and their role as antioxidant and antibacterial agents. Three common solvents such as petroleum ether (40° - 60°C), chloroform and methanol were successively used for the extraction of active principles from sea buckthorn berries. Five major fractions (F1-F5) were isolated from the active methanol extract by column and thin layer chromatography. An attempt was made to identify the chemical nature of pooled fractions by available spectral means. Antioxidant potential of methanol extract and its fractions was measured by DPPH, formation of phosphomolybdenum complex and TBA methods. The hole-plate diffussion method was used to find out the antibacterial activity. A very brief structure-activity relationship of the potent antioxidant and antimicrobial compounds is discussed. Methanolic extract and its fractions contain numerous phenolic compounds such as flavonoids, which may be responsible for antioxidant and antibacterial effects.

  16. Preparation of sea buckthorn vinegar beverage%沙棘果醋饮料的研制

    Institute of Scientific and Technical Information of China (English)

    牛广财; 朱丹; 魏文毅; 王欣; 王鹤霖; 杨建

    2012-01-01

    以沙棘汁为主要原料,经酒精发酵、醋酸发酵制成沙棘原醋,再调配成沙棘果醋饮料。以沙棘原醋用量、沙棘果汁用量、蜂蜜用量、蔗糖糖浆用量为实验因素,以感官评分为考察指标,研究了沙棘果醋饮料的配方和加工工艺。经正交实验得出每100mL沙棘果醋饮料的最佳配方为:沙棘原醋8mL,沙棘果汁15mL,蜂蜜3g,蔗糖糖浆10g。经过调配、过滤、灌装后,在100℃下杀菌5rain。生产的沙棘果醋饮料色泽亮丽,酸甜适口,具有浓郁的沙棘果香、醋香和蜂蜜香气。%Sea buckthorn vinegar was made from sea buckthorn juice by alcoholic and acetic fermentations,which was then formulated to a sea buckthorn vinegar beverage.With the dosages of sea buckthorn vinegar,juice, honey and sucrose syrup as the experimental factors and the sensory scores of the sea buckthorn vinegar beverage as the evaluation indexes,the formula and processing technology were researched on.By orthogonal experiment,the optimum formula of the vinegar beverage was determined as follows:sea buckthorn raw vinegar 8mL/100mL, sea buckthorn juice 15mL/100mL, honey 3g/100mL and sucrose syrup lOg/100mL.The optimal sterilization condition of the product was holding on 100℃ for 5min after formulation,filtering and filling.The sea buckthorn vinegar beverage with bright color,sweet and sour taste, had sea buckthorn flavor,vinegar and honey aromas.

  17. Achievement of a Fortifying Product based on Apples, Quinces and Sea Buckthorn

    Directory of Open Access Journals (Sweden)

    Adriana VOICU

    2009-06-01

    Full Text Available The aim of the research was to achieve a fortifying product Apple, Quince and Sea Buckthorn Nectar rich in antioxidants (vitamin C, ?-carotene and dietary fibers (cellulose, pectic substances from fruit. In the experiments were used fruit (apples, quinces and sea buckthorn with a high content of these nutrients. The antioxidants are chemical substances that inhibit, decompose or prevent forming of free radicals or other substances that induce the cancerogenesis. Also, dietary fibers are another group of natural compounds with important role in prevention of the digestive diseases. In the experiments were used biochemical methods for analysis of fruit and processed products (determination of soluble dry substance, glucides, proteins, lipids, mineral elements, total acidity, vitamin C, ?-carotene, cellulose, pectic substances etc.. Also were performed microbiological analysis and sensorial analysis for processed products.In order to obtain the product Apple, Quince and Sea Buckthorn Nectar, four variants of experiments were carried out at the laboratory level. Following the sensorial and biochemical analyses, the optimum variant of the product Apple, Quince and Sea Buckthorn Nectar was selected, namely the variant V3 in which the ratio apple puree and quince puree was 1:1 and the percentage of added sea buckthorn juice was of 3%.

  18. 沙棘叶片内过氧化氢酶的研究%Study on Catalase in Leaves of Hippophae rhamnoides Linn

    Institute of Scientific and Technical Information of China (English)

    何士敏; 方平; 何莉

    2010-01-01

    [目的]研究沙棘叶片内过氧化氢酶(CAT)的酶活力和酶催化特性,为沙棘的开发利用提供参考.[方法]采用紫外分光光度法,对沙棘叶片内CAT活性进行测定,并研究温度、pH值、激活剂和抑制剂对其活性的影响.[结果]沙棘叶片CAT的最适温度在20~30 ℃之间,最适pH值为7.0.Mg2+、Cu2+和SDS对沙棘CAT有激活作用,而EDTA、Fe2+、Ca2+、Ag+、Mn2+和Fe3+则有较强的抑制作用.在pH值为7.0,温度为30 ℃时,CAT活性可达1 733 U/g·min;[结论]沙棘叶片内过氧化氢酶具有很强的活性,温度、pH值、激活剂和抑制剂对其有明显影响.

  19. Identification of urinary biomarkers after consumption of sea buckthorn and strawberry, by untargeted LC-MS metabolomics

    DEFF Research Database (Denmark)

    Cuparencu, Catalina Sinziana; Andersen, Maj-Britt Schmidt; Gürdeniz, Gözde;

    2016-01-01

    , strawberry and sea buckthorn, in humans. A randomized controlled single-blinded three-way cross-over meal study was conducted in 16 overweight men. The intervention meals consisted of sea buckthorn puree, strawberry puree or an iso-caloric control drink. Urine samples were collected on each test day at t...... in very low concentrations and therefore not identified. Nine and 11 markers were (tentatively) identified for strawberry and sea buckthorn, respectively, the most specific being conjugates of aroma compounds and coloring flavonoids. Metabolites reflecting the step-wise aromatization of quinic to hippuric...... profiling after consumption of sea buckthorn or strawberries. Combinations of these markers are proposed as potentially specific for intake of each of these berries, but need further validation in larger and less-controlled study settings....

  20. The content determination of calcium, magnesium and ascorbic acid in sea buckthorn fruits at vulcan coal dump

    Directory of Open Access Journals (Sweden)

    Andreea BRASOVAN

    2009-11-01

    Full Text Available The study presents data on content determination of calcium, magnesium and ascorbic acid in sea buckthorn fruits collected from Vulcan coal dump. The dump was never covered with soil or fertilizers containing azoth, potassium and phosphorus. The sea buckthorn was planted directly on the sterile and it has an important function in stabilizing the acclivity and fixing the atmospheric azoth with the nodosities on the roots. So, the sea buckthorn becomes widespread plant in coal dumps because it helps to the soil formation. The results obtained after the determination of calcium, magnesium and ascorbic acid from the fruits of this plant were compared with the values already reported in various references, concluding that these differences are insignificant (3.7% for magnesium and 9.4% for calcium. Therefore, the sea buckthorn from the dump can be used in food industry, as well as in pharmaceutics and medicine.

  1. Protection against vascular endothelial dysfunction by polyphenols in sea buckthorn berries in rats with hyperlipidemia.

    Science.gov (United States)

    Yang, Fang; Suo, Yourui; Chen, Dongli; Tong, Li

    2016-07-19

    Chronic hyperlipemia increases the incidence of vascular endothelial dysfunction and can even induce cardiovascular disease. Sea buckthorn contains a host of bioactives such as flavonoids and polyphenols that can prevent the development of cardiovascular disease. The current study isolated active ingredients, polyphenols, from sea buckthorn berries (SVP) and orally administered SVP at a dose of 7-28 mg/kg. This treatment significantly reduced serum lipids, it enhanced the activity of antioxidant enzymes, and it decreased the level of serum TNF-α and IL-6. SVP also alleviate vascular impairment by decreasing the expression of eNOS, ICAM-1, and LOX-1 mRNA and proteins in aortas of rats with hyperlipidemia. Based on these findings, SVP has antioxidant action and it protects endothelium.

  2. Preliminary toxicology evaluation of Hippophae rhamnoidesmarc flavones%沙棘果皮渣黄酮毒理学初步评价

    Institute of Scientific and Technical Information of China (English)

    张凤清; 张东生; 周雅茹

    2012-01-01

    The acute toxicity and genetic toxicity of Hippophae rhamnoides were performed by acute oral toxicity test in ICR mice,such as chromosome aberration test of bone marrow cells of mice,the micronucleus test of bone marrow cells of mice and the sperm abnormality text of mice.The result showed that the oral maximum tolerance dose of mice(MTD)was 24g/kg.According to acute toxicity classification criteria,test substance belongs to non-toxic.Under the dose of less than 24g/kg,high dose group(8g/kg),middle dose group(4g/kg)and low dose group(2g/kg)were set up to check the genetic toxicity of test substance.Result showed that the chromosome aberration rate,the micronucleus rate and the sperm abnormality rate didn't increase,what improved that test substance would not cause the aberration of chromosome,increase the micronucleus amount of mice polychromatic erythrocyte and lead to sperm abnormality.%以沙棘果皮渣黄酮为受试物,经口染毒针对ICR小鼠做急性毒性实验、小鼠骨髓细胞染色体畸变实验、小鼠骨髓细胞微核实验、小鼠精子畸变实验,对受试物进行急性毒性实验及初步遗传毒性评价。实验结果表明:小鼠经口染毒最大耐受量(MTD)为24g/kg(〉15g/kg),受试物的急性毒性分级为无毒级;在MTD以下分设高(8g/kg)、中(4g/kg)、低(2g/kg)三个剂量组考察受试物的遗传毒性,实验结果为小鼠骨髓染色体畸变率、小鼠骨髓细胞微核率以及小鼠精子畸变率均未发现增高,表明受试物不能导致小鼠骨髓细胞染色体畸变,不会诱发小鼠嗜多染红细胞微核产生,对小鼠精子无致畸作用。

  3. Effect of sea buckthorn and vitamin E on growth performance of Acipenser stellatus (Pallas 1771 juveniles

    Directory of Open Access Journals (Sweden)

    Oana Georgiana Dorojan

    2015-05-01

    Full Text Available Addition of natural additives (prebiotics, probiotics, phytobiotics in fish feed is a benefic method because it leads to improved growth performance of fish reared in intensive production systems. Sea buckthorn is one of the most commonly phytobiotics used in aquaculture because of its antioxidant, antimicrobial, immunostimulant, anti-stress, cytoprotective, liver and tissue regeneration properties. Sea buckthorn berries are rich in vitamins (B, C, E and K, flavonoids, carotenoids, tocopherols and many volatile compounds. In this context the aim of this study was to evaluate the effects of dietary vitamin E (500 mg/kg diet and sea buckthorn on growth performance and survival of the A. stellatus juveniles reared in a recirculating aquaculture system. The experiment took place in the recirculating pilot system of “Dunărea de Jos” University of Galați. In order to emphasize the influence of the above mentioned immunostimulants on sevruga fingerlings with different genetic background, four experimental groups from different genitors (V1: ♀2 Danube x ♂1 Aquaculture, V2: ♀1 Danube x ♂1 Danube, V3: ♀1 Danube x ♂2 Aquaculture and V4: ♀2 Danube x ♂2 Danube have been used. At the end of the experiment no significant differences (p>0.05 were observed in terms of final mean weight, individual growth rate, SGR, FCR, PER, between the sevruga fingerlings obtained from wild genitors, comparing with those obtained from aquaculture genitors.

  4. Above-ground biomass models for Seabuckthorn (Hippophae salicifolia) in Mustang District, Nepal

    DEFF Research Database (Denmark)

    Rajchal, Rajesh; Meilby, Henrik

    2013-01-01

    Seabuckthorn (Hippophae salicifolia D. Don.), an important multi-purpose tree, is found at altitudes of 2000–3600 m amsl in Nepal, but so far no models have been developed for estimating the biomass of this species, thus hampering resource assessment and management planning. Hence, the objective...

  5. Studies on binding mechanism between carotenoids from sea buckthorn and thermally treated α-lactalbumin

    Science.gov (United States)

    Dumitraşcu, Loredana; Ursache, Florentina Mihaela; Stănciuc, Nicoleta; Aprodu, Iuliana

    2016-12-01

    Sea buckthorn is a natural food ingredient rich in bioactive compounds such as carotenoids, tocopherols, sterols, flavonoids, lipids, vitamins, tannins and minerals. Herein, fluorescence and UV-vis techniques were used to study the interaction of heat treated α-lactalbumin (α-LA) with carotenoids from sea buckthorn berries extract (CSB) and β-carotene. Further atomic level details on the interaction between α-LA and β-carotene were obtained by means of molecular modelling techniques. The quenching rate constants, binding constants, and number of binding sites were calculated in the presence of CSB. The emission spectral studies revealed that, CSB have the ability to bind α-LA and form a ground state complex via static quenching process. Maximum degree of quenching was reached at 100 °C, where β-carotene and CSB quenched the Trp fluorescence of α-LA by 56% and 47%, respectively. In order to reveal the interaction between CSB and α-LA, the thermodynamic parameters were determined from the van't Hoff plot based on the temperature dependence of the binding constant. In agreement with the in silico observations, the thermodynamic parameters enabled us to consider that the association between α-LA and β-carotene is a spontaneous process driven by enthalpy, dominated mainly by the van der Waals interaction, but hydrophobic interactions might also be considered. The interaction between CSB and α-LA was further confirmed by UV-vis absorption spectra, where a blue shift of position was noticed at higher temperature suggesting the complex formation. The results provided here supply a better understanding of the binding of CSB to α-LA, which can be further exploited in designing new healthy food applications.

  6. Strategy of experimental design for intensification of solvent extraction of natural antioxidant flavonoids and phenols from buckthorn textured leaves

    Directory of Open Access Journals (Sweden)

    Baya Berka

    2015-12-01

    Full Text Available Prior to solvent extraction of plant-based active molecules, adequate texturing by Détente Instantanée Contrôlée (DIC; French for “Instant controlled pressure drop” results in overcoming the slow diffusion of the solvent/solute through the solid matrix. This work aimed at determining the impact of DIC pretreatments on buckthorn (Rhamnus alaternus L. morphology. DIC-operating parameters were selected as the saturated steam pressure, the thermal treatment time, and the number of cycles. A three-parameter five-level response surface method was used to optimize DIC processing parameters. Response factors were the overall and individual yields of flavonol aglycone extraction, and antioxidant activity of both expanded dried material (swell dried leaves and extracts. The yield of flavonol aglycones was 18.23 mg Kaemp eq/g dry basis (mg Kaemp eq/g db in 3 min for DIC-treated buckthorn, against 12.24 mg Kaemp eq/g db in 150 min for untreated natural buckthorn raw material. Furthermore, the antioxidant activity of DIC-treated material was exceptionally higher and the effectiveness of reducing power of DPPH radical was 68 times more than the untreated plant material.

  7. Investigation on the antidepressant effect of sea buckthorn seed oil through the GC-MS-based metabolomics approach coupled with multivariate analysis.

    Science.gov (United States)

    Tian, Jun-sheng; Liu, Cai-chun; Xiang, Huan; Zheng, Xiao-fen; Peng, Guo-jiang; Zhang, Xiang; Du, Guan-hua; Qin, Xue-mei

    2015-11-01

    Depression is one of the prevalent and serious mental disorders and the number of depressed patients has been on the rise globally during the recent decades. Sea buckthorn seed oil from traditional Chinese medicine (TCM) is edible and has been widely used for treatment of different diseases for a long time. However, there are few published reports on the antidepressant effect of sea buckthorn seed oil. With the objective of finding potential biomarkers of the therapeutic response of sea buckthorn seed oil in chronic unpredictable mild stress (CUMS) rats, urine metabolomics based on gas chromatography-mass spectrometry (GC-MS) coupled with multivariate analysis was applied. In this study, we discovered a higher level of pimelic acid as well as palmitic acid and a lower level of suberic acid, citrate, phthalic acid, cinnamic acid and Sumiki's acid in urine of rats exposed to CUMS procedures after sea buckthorn seed oil was administered. These changes of metabolites are involved in energy metabolism, fatty acid metabolism and other metabolic pathways as well as in the synthesis of neurotransmitters and it is helpful to facilitate the efficacy evaluation and mechanism elucidating the effect of sea buckthorn seed oil for depression management.

  8. Above-ground biomass models for Seabuckthorn (Hippophae salicifolia) in Mustang District, Nepal

    DEFF Research Database (Denmark)

    Rajchal, Rajesh; Meilby, Henrik

    2013-01-01

    Seabuckthorn (Hippophae salicifolia D. Don.), an important multi-purpose tree, is found at altitudes of 2000–3600 m amsl in Nepal, but so far no models have been developed for estimating the biomass of this species, thus hampering resource assessment and management planning. Hence, the objective...... of this study was to develop local biomass models for wood, fruit, and leaves of Seabuckthorn. In November 2006, a diameter-stratified sample of 30 trees was harvested in Lete and Kunjo Village Development Committees at an altitude of about 2300 m amsl in the lower part of Mustang District, Nepal. The fresh...... weight of fruit and oven-dry weight of wood (stem and branches) and leaves were measured and used as a basis for developing biomass models. Diameters of the trees were measured at 30 cm above ground whereas the heights were measured in terms of the total tree height (m). Among several models tested...

  9. 复合溶剂提取沙棘籽油的工艺条件研究%Extraction of seabuckthorn seed oil with mixed solvent from Hippophae rhamnoides seed

    Institute of Scientific and Technical Information of China (English)

    张丽霞; 康健; 吴桐

    2011-01-01

    采用响应面优化了正己烷-丙酮复合溶剂浸提沙棘籽油的提取条件,并对沙棘籽油的理化性质进行分析.结果表明:最优条件为料液比1∶14、粉碎度35.00目、正己烷:丙酮为6∶4、浸提温度44.00℃、浸提时间1.84h,在该条件下沙棘籽油提取率为13.788%.沙棘籽油的碘价为156.30mg/100g,酸价为8.80mg KOH/g,皂化值为186.30mg KOH/g,水分及挥发物0.22%,过氧化值为9.21meq/kg,不皂化物含量为1.89%.%Extraction conditions of seabuckthom seed oil with mixed solvents were optimized with response surface methods (RSM) and physiochemical properties of crude oil were analysed. The optimum conditions were as follows: ratios of solid to liquid 1:14, grinding size 35.00 mesh,mixed solvent as nhexane to acetone 6:4, extraction temperature 44.00℃ and extraction time 1.84h. The extraction rate of seabuckthorn seed oil was 13.788% under the optimum conditions. The iodine value, acid value ( AV ) and saponification value of seabuckthorn seed oil were 156. 30mg/100g, 8.80g KOH/g and 186. 30mg KOH/g, respectively. And the water volatiles, peroxide value and unsaponifiable value were 0.22%, 9.21meq/kg, and 1.89%, respectively.

  10. 国外生态经济树种沙棘引种驯化及早期评价%Introduction and Domestication as Well as Early Evaluation of Hippophae rhamnoides of Foreign Ecological and Economic Species

    Institute of Scientific and Technical Information of China (English)

    崔云英; 兰士波; 温爱亭

    2011-01-01

    为丰富我国高寒地区生态经济林树种遗传多样性,促进林业生态体系和产业体系建设,遵循供种区与引种区环境条件相似的原则,根据引种区气候、土壤、植被分布、地形地貌及地理位置等主要环境条件,确定与之相适宜俄罗斯为供种区。以6个俄罗斯大果沙棘优良品种为引种对象,在引种驯化及区域试验的基础上,综合分析与评价子代生态适应性、生长表现及果实品质,确定YSH和HJN为高寒地区引种栽培的适宜品种,且品种内家系间数量性状变异较小,生长稳定,具广泛推广应用价值。%In order to enrich the resources ot PLcological and Economlc iorest species in the cold area,develop variety of the creature and accelerate the construction of ecological and industrial system, Following resemble principle between the environmental conditions of seed zone and introduction area, The areas introducing fine varieties in the cold area confirm to regard the suitable the Russia as the area of origin ac- cording to its own climate, soil, vegetation distribution, landform and the general configuration of the earth's surface, geographical position and the other main environmental situations. The Russia's Seabuckthorn improved varieties was employed to analyze and evaluate the adaptability, growth performance of seeding and fruit quality of foreign ecological and economic species was farther studied based on regionalization test, introduction and domestication. It was held preliminarily that two varieties of YSH, HJN grew well and the growth of quantitative character varied slightly within families which remained stable, and possess extensive generalization and utilization value.

  11. 鸟类取食中国沙棘果实的方式及其对种子的传播作用%Fruits foraging patterns and seed dispersal effect of frugivorous birds on Hippophae rhamnoides sinensis

    Institute of Scientific and Technical Information of China (English)

    鲁先文; 孙坤; 马瑞君; 张辉; 苏雪; 王明理

    2005-01-01

    2003年9月~2004年3月,定期观察取食中国沙棘果实的鸟类及其取食方式.共记录取食中国沙棘果实的鸟类18种,其取食果实的方式主要有:1)直接在树冠上吞食果实,有时候在吞食后将种子呕出;2)将果实从树上衔走后,在栖息处吞食或啄食;3)将果实啄落至地面,然后取食果肉和种子,留下果皮;4)啄破果皮,吸食果肉,留下果皮及种子;5)从顶端将果皮啄破后,仅取食里面的种子.不同的取食方式决定了它们对沙棘种子的传播作用不同.收集鸟粪便中的种子与完整的干果实及人工剥离果肉和内果皮的种子做萌发对比实验,结果表明,鸟类消化道过程对种子的萌发有一定影响,但萌发率仍较高.沙棘为多种鸟类提供食物,而鸟类则为沙棘传播种子,它们之间形成一种互利关系.

  12. Study on the impact of fermentation process to sea buckthorn icewine quality%发酵条件对沙棘冰酒品质的影响

    Institute of Scientific and Technical Information of China (English)

    李树志; 鲁长征; 山永凯; 刘洪智; 任蓓蕾

    2009-01-01

    The sea buckthorn juice was used as raw material, and the fermentation process parameters of ice sea buckthorn wine were studied, including color, organization, smell and taste. Through orthogonal experiment, the best fermentation process parameters of ice sea buckthorn wine could be gotten:fermentation temperature 24 ℃, yeast inoculum 3% (V/V, 106/mL), pH 3. 6, fermentation time 20 d. Under these conditions, the ice sea buckthorn wine had better color, organization, smell, taste and suitable for large-scale production.%以沙棘汁为原料研究发酵工艺中各参数对沙棘冰酒品质的影响.通过L9(34)正交试验,得出制备沙棘冰酒的最佳发酵工艺参数为:发酵温度24℃,酵母接种量3%(V/V,106个/mL),PH 3.6,发酵时间20 d.在此条件下,沙棘冰酒的色泽、组织状态、滋味、口感均较理想,适合企业规模化生产.

  13. Study on Cuttings Container Seedling-raising of Hippophae thibetana Schlecht%西藏沙棘容器扦插育苗研究

    Institute of Scientific and Technical Information of China (English)

    春雨

    2012-01-01

    [Objective] Aiming at acutual condition of development and utilization of Hippophae thibetana Schlecht, design the asexual reproduction cutting method study the cuttings container seedling-raising of Hippophae thibetana Schlecht, in order to maintain its excellent shape. [ Method ] Study the effects of 50,100,150,200 μg/ml GGR on survival rate, root quantity, root length of Hippophae tibetana Schlecht. [Result] 1 year old cutting of Hippophae tibetana Schlecht have a higher survival rate in the concentration of 100 μg/ml and cutting depth of 1/2 cuttings. [ Conclusion] 100 μg/ml GGR was suitable for the local Hippophae tibetana Schlecht.%[目的]针对西藏沙棘开发利用的现状,为了保持其优良性状,设计无性扦插繁育方式,分析研究西藏沙棘容器扦插育苗的基本方法.[方法]研究50、100、150、200μg/ml GGR溶液对西藏沙棘扦插成活率、生根数、根长的影响.[结果]西藏沙棘1年生嫩枝扦插,在GGR浓度为100 μg/ml,扦插深度是插条长度的1/2时有较高的成活率.[结论]浓度为100μg/ml GGR溶液适合于当地西藏沙棘扦插的催根处理.

  14. Cutin composition of five finnish berries.

    Science.gov (United States)

    Kallio, Heikki; Nieminen, Riikka; Tuomasjukka, Saska; Hakala, Mari

    2006-01-25

    The raw cutin (i.e., extractive-free isolated cuticular membrane) fraction from Finnish berries, sea buckthorn (Hippophaë rhamnoides), black currant (Ribes nigrum), cranberry (Vaccinium oxycoccos), lingonberry (Vaccinium vitis-idaea), and bilberry (Vaccinium myrtillus), was depolymerized by NaOMe-catalyzed methanolysis. The composition of cutin monomers was determined by GC-(EI)MS analysis either as methyl esters or as TMSi esters, with OH groups derivatized to TMSi ethers. There was a notable difference in the degree of depolymerization, ranging from 6 to 47%. The extractive-free berry cuticle, that is, raw cutin, thus contains cutin. The predominant cutin monomers were C(16) and C(18) omega-hydroxy acids with midchain functionalities, mainly epoxy and hydroxyl groups. Typically, the major compounds were 9,10-epoxy-18-hydroxyoctadecanoic acid, 10,16-dihydroxyhexadecanoic acid, 9,10,18-trihydroxyoctadecanoic acid, 9,10-epoxy-18-hydroxyoctadec-12-enoic acid, and 18-hydroxyoctadec-9-enoic acid. The amount of epoxyacids was rather high in sea buckthorn ( approximately 70%) and cranberry ( approximately 60%), compared with the other berries. The black currant cutin differed from that of the other berries with a significant portion of hydroxyoxohexadecanoic acid ( approximately 12% of total monomers). This investigation of the cuticular hydroxy acids of five Finnish berries is part of the exploitation of the northern natural resources related to the chemical composition, nutritional value, and sensory properties.

  15. Development and Evaluation of Set-type Sea Buckthorn Yogurt%凝固型沙棘酸奶的研制及其评价

    Institute of Scientific and Technical Information of China (English)

    林祥群; 马彩梅; 杨国江; 吴宏; 刘文玉

    2016-01-01

    【目的】研究不同沙棘果汁添加量、蔗糖添加量、菌种接种量和发酵时间对沙棘酸奶品质的影响,为开发凝固型沙棘酸奶提供依据和参考。【方法】通过单因素预试验,研究沙棘果汁添加量、蔗糖添加量、菌种接种量和发酵时间的合理取值范围;通过四因素三水平正交试验研究沙棘酸奶的最佳工艺配方组合;固定蔗糖添加量为10%、菌种接种量为4%和发酵时间为4 h,研究不同沙棘果汁添加量对酸奶中VC和蛋白质含量的影响。【结果】果汁添加量10%、蔗糖添加量10%、菌种接种量4%、发酵时间4 h的沙棘酸奶风味突出、酸甜爽口、色泽诱人、组织状态良好,是制作凝固型沙棘酸奶最为理想的工艺配方组合;沙棘酸奶中VC含量是普通原味酸奶的2.3~4.8倍并与果汁添加量呈正相关关系,符合函数y=-59.048 x2+30.623 x+1.0074(R2=0.991);蛋白质含量与果汁添加量呈负相关关系。【结论】沙棘酸奶具有典型的沙棘果香风味,制作沙棘酸奶的最佳工艺配方为果汁添加量10%、蔗糖添加量10%、菌种接种量4%、发酵时间4 h,其VC含量为3.67 mg/100 g、蛋白质含量为2.91%,具有较高的营养价值。%Objective]From the aspect the development of set-type sea buckthorn yogurt,this paper e-laborated related processing technology,and studied the effect of sea buckthorn juice addition on yogurt VC and protein content.[Method]the reasonable range of values of juice dosage,the amount of sugar added,in-oculation amount and fermentation time.This paper made orthogonal experiment to study the effects of sea buckthorn juice dosage,the amount of sugar added,inoculation amount and fermentation time on set-type sea buckthorn yogurt quality.The orthogonal test of four factors at three different levels was used to study the optimum ratio.The paper also researched the effect of sea buckthorn juice

  16. Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion

    Institute of Scientific and Technical Information of China (English)

    Santhrani Thakur; Pradeepthi Chilikuri; Bindu Pulugurtha; Lavanya Yaidikar

    2015-01-01

    Objective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were pretreated with alcoholic extract of H. salicifolia (250 and 500 mg/kg) for 14 d and focal cerebral ischemia was induced by MCAO. After 60 min of MCAO, reperfused for 24 h, a battery of behavioral tests were assessed the extent of neurological deficits. Infarct volume and brain edema were measured in 2,3,5-triphenyltetrazolium chloride stained brain sections. TNF-α, oxidative stress parameters like reduced glutathione, calcium, glutamate, malondialdehyde and apoptotic parameters like caspase-3, and caspase-9 were estimated in the brain homogenates. Results:Pretreatment with alcoholic extract of H. salicifolia at doses of 250 and 500 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema induced by ischemia reperfusion injury. H. salicifolia significantly prevented ischemia induced increase in malondialdehyde, glutamate, calcium, caspase-3, caspase-9 and TNF-αlevels as compared to ischemic animals. Conclusions: Our results indicate that H. salicifolia mitigated the ischemia reperfusion induced neuronal damage.

  17. 沙棘属植物种子微形态特征研究%Micromorphological Features of the Seed Surface of the Genus Hippophae L.

    Institute of Scientific and Technical Information of China (English)

    庞海龙; 苏雪; 张辉; 蔡正旺; 孙坤

    2012-01-01

    应用解剖镜和扫描电镜对沙棘属(Hippophae Linn.)6种6亚种植物种子形态、种皮微形态特征进行了观察.结果表明:沙棘属植物种子形态、种皮微形态等性状具有一定程度的稳定性,同时种间存在明显的差异,对于种及种下类群的分类、鉴定有重要参考价值.其中种皮微形态可分为4种类型:拟脑纹状纹饰、线纹-穴状纹饰、近平滑-细疣状纹饰、浅穴-疣状纹饰.从内果皮和种皮结合程度及种皮表面光泽一致性等方面可明显地将沙棘属植物划分为二者紧密结合与分离两种情况,基本支持将沙棘属植物划分为有皮组和无皮组两大类群的观点,但观察结果存在一些差异.%Seed morphology and micromorphology of the seed coat of six species and six subspecies of Hippophae L from China were examined by stereoscope and scanning electron microscope (SEM). The results showed that these species were obviously different in seed shape and seed coat micromorphology. These micromorphological features were important in their classification. The seed surface morphologies were divided into four types: paracere-broid, lineate-foveate, nearly smooth-fine verrucose, and shallow foveate-verrucose. Degree of separation of endocarp and seed coat indicated that they were also divided into two types: close combination and separation. The results of the study partly support Lian's viewpoint that Hippophae is divided into two sections: Sect. 1. Hippophae and Sect. 2. Gyantsenses, but also showed there were some differences.

  18. Study on Biological Feed of Sea-buckthorn Manufactured by Complex Microbial Flora%复合微生物菌群生产沙棘生物饲料的研究

    Institute of Scientific and Technical Information of China (English)

    金敬红; 吴素玲; 孙晓明

    2015-01-01

    This article studied the production technology of Sea-buckthorn biological feed using complex microbial flora consisted of Bacillus subtilis and beer yeast based on the Sea-buckthorn fruit slag, deter-mine the best proportion of complex microbial flora and study the inoculation proportion, pH of raw mate-rial and fermentation cycle.%以沙棘果渣为原料,研究了以枯草芽孢杆菌和啤酒酵母组成的复合微生物菌群生产沙棘生物饲料的工艺,确定了复合微生物菌群的最佳配比,对接种比例、原料pH以及发酵周期进行了研究。

  19. Effect of Original Juice Treatment Before Fermentation on the Amino Acid of Sea-buckthorn and Raspberry Ice Wine%发酵前处理对沙棘树莓冰酒氨基酸的影响

    Institute of Scientific and Technical Information of China (English)

    马荣山; 郭宇

    2013-01-01

    Fruit wine was prepared by using sea-buckthorn and raspberry as raw materials, mixed fruit juice by D 101-I resin, processed orange juice and original juice were regulated to original sugar concentration of 12% and pH 4.2. Then added active dry yeast of 1.2‰,the process conditions of sea-buckthorn and raspberry wine fed-batch fermentation were optimized. In this study, the sea-buckthorn wine was detected after aging by the amino acid analyzer. It was found that The amino acid content of processed orange juice were relatively more.The sea buckthorn and raspberry wine browning can be controlled. Stability was improved, the taste was improved.%  以沙棘汁和树莓汁为原料,混合果汁经 D 101-I 大孔吸附树脂过滤,分别对处理过的果汁和未处理的果汁调整初始糖度12,pH4.2,接入1.2‰活性干酵母,采用流加发酵优化发酵工艺,经陈酿得到沙棘树莓冰酒。本实验采用 L-8800全自动氨基酸分析仪对两种发酵冰酒的氨基酸成分进行检测。结果表明发酵前对混合果汁用树脂处理,氨基酸含量相对多些,与此同时能有效控制沙棘树莓酒陈酿过程中的褐变,提高稳定性,改善酒的口感。

  20. Comparative Fingerprint of Aromatic Herbs and Yeast Alcoholic Extracts used as Ingredients for Promen, a Prostate Preventive Nutraceutical

    Directory of Open Access Journals (Sweden)

    Florina Csernatoni

    2013-11-01

    Full Text Available The aim of this study was to characterize and identify different bioactive compounds in plant sources and yeast powders  to obtain an original nutraceutical (Promen which has beneficial effects in prostate disease prevention. Seven plant and fruit sources, namely nettle (Urtica dioica, green tea (Camellia sinensis, fluff with small flowers (Epilobium parviplorum, tomato (Solanum licopersicum,  sea buckthorn (Hippophae rhamnoides, pumpkin (Cucurbita maxima, sunflower (Helianthus annus and lyophilized beer yeast (Saccharomyces cerevisiae were investigated. Methanolic extracts were prepared using 15% plant concentration and the purified fractions were analyzed using high throughput techniques like UV-VIS spectroscopy, high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD and mass spectrometry LC-QTOF -MS. The majority of the investigated plants were rich in phenolic derivatives, polyphenols (flavonoid glucosides, while yeast was rich in aminoacids, peptides and vitamins B. The major compounds identified were: Juglone, Resveratrol, Quercetin, Epigallocatechin, Gallocatechin, Biochanin A, Isorhamnetin 3-O-glucoside 7-O-rhamnoside, Quercetin 3-O-galactoside 7-O-rhamnoside, Kaempferol 3,7-O-diglucoside and p-Coumaroylquinic acid. The specific biomarkers were identified for both plant extracts used as ingredients to obtain an nutraceutical  Promen. Combined UV-Vis spectroscopy, HPLC-PDA chromatography and LC-MS spectrometry are recommended as accurate, sensible and reliable tools to investigate the plants and nutraceutical fingerprints and to predict the relation between ingredients composition and their health effects.

  1. The Influence of Some Phytobiotics on Haematological and Some Biochemical Indices at Oreochromis Niloticus – Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Alina Antache

    2014-05-01

    Full Text Available The aim of this research was to evaluated the influence of some phytobiotics on haematological profile, leukocyte reaction and some biochemical indices at Oreochromis niloticus species, reared in a recirculating aquaculture system. This experiment was conducted six weeks. The experimental variants were: V1 – control; V2 – 1% Rosmarinus officinalis / kg feed; V3 – 1% Hippophae rhamnoides / kg feed and V4 – 1% Zingiber officinale / kg feed. Blood was analyzed using standard techniques. At the end of the experiment the following parameters were determined: RBCc (x106cells/µL, Hb (g/dL, PVC (%, MCV (µm3, MCH (pg, MCHC (g/dL, TP (g/dL, GLU (mg/dL, cortisol (ng/mL, lysozyme activity (U/mL, absolute number of blood cells (x103 cells/µL and leukogram (%. The results showed that the administration in feed of some phytobiotics lead to signifiant differences (p<0.05 of following parameters: RBCc (x106cells/µL, MCV (µm3, glucose (mg/dL, lysozyme activity (U/mL, monocyte (% and in absolute number of leukocytes, lymphocytes and monocytes. In conclusion, due to decreasing of RBCc, PVC, Hb, MCHC, cortisol, GLU and due to normal concentration of TP, we can say that the administration of sea buckthorn and ginger, but even rosemary administration, in diet improves the physiological status at Oreochromis niloticus species.

  2. Study on influence factors on malolactic fermentation technology of sea buckthorn wine%沙棘果酒苹果酸-乳酸发酵影响因素研究

    Institute of Scientific and Technical Information of China (English)

    邢玮; 韩建春

    2012-01-01

    Taking sea buckthorn as material, sea buckthorn wine was prepared by oeni. The optimal conditions of fermentation were given as follows: the pH was 3.4, and the fermentation temperature was 25 °C, and the inoculation was 8%. After the alcohol secondary fermentation, the strain was inoculated, SO2 concentration was below 50 mg- L-1, the fermentation time was 8 d. After malolactic fermentation , the total acid of the sea buckthorn wine decreased from 15.5 g ? L-1 to 9.89 g ? L-1. According to the conditions, malic acid of the sea buckthorn was translated into the lactic acid, and the sour taste of sea buckthorn wine was weakened and the taste was greatly improved.%以沙棘果为原料,利用酒酒球菌降酸生产全汁沙棘果酒,通过单因素正交试验确定酒精发酵的最佳条件为:pH值3.4,发酵温度25℃,接菌量8%,接种时间为酒精发酵后(顺序发酵),SO2浓度30 mg·L-1,发酵时间为8d.苹果酸-乳酸发酵(MLF)结束后,沙棘果酒的总酸由原来的15.20 g·L-1降为9.83 g·L-1,苹果酸降解量5 422.07 mg·L-1、挥发酸增加量261 mg·L-1.在此工艺条件下酿制的沙棘果酒不仅使苹果酸大部分转化为乳酸,并且沙棘果酒的酸涩味减弱,口感得到很大的改善.

  3. 醋柳黄酮口服固体脂质纳米粒对小鼠靶向性的研究%Studies on the Targeted Effect of Total Flavones of Hippophae Rhamnoides in Solid Lipid Nanoparticles on Mice in Vivo

    Institute of Scientific and Technical Information of China (English)

    石文宏; 巩俊卿; 盛惟

    2006-01-01

    目的:研究醋柳黄酮口服固体脂质纳米粒(TFH-SLN)在小鼠体内的靶向性及吸收、分布的经时变化规律.方法:采用HPLC法测定小鼠口服醋柳黄酮(TFH)和TFH-SLN后血液、心、肝、脾、肾的药物浓度.结果:TFH-SLN在心、血、肝中的分布明显高于THH.结论:TFH-SLN具有明显的心、肝、血靶向性.

  4. 二倍体杂交种棱果沙棘双向杂交起源及其母本主要来源于中国沙棘的分子证据%Molecular Evidence for Bidirectional Hybrid Origin and Hippophae rhamnoides ssp.sinensis as the Mainly Maternal Plant of the Diploid Hybrid H.goniocarpa (Elaeagnaceae)

    Institute of Scientific and Technical Information of China (English)

    蒋严妃; 严容; 苏雪; 陈纹; 孙坤

    2014-01-01

    棱果沙棘为同域分布的中国沙棘和肋果沙棘同倍化自然杂交形成的.本文利用母系遗传的cpDNA trnS-G序列检测青海祁连棱果沙棘及其亲本中国沙棘和肋果沙棘同域分布的两个地区(拱北湾、八宝河滩)共93个个体的遗传关系.结果表明棱果沙棘及其亲本在拱北湾和八宝河滩分别有12个和7个单倍型,两地区的棱果沙棘都与其亲本共享单倍型,其中拱北湾棱果沙棘共36个个体中有28个与中国沙棘共享3个单倍型(H2,H4,H5),有2个个体与肋果沙棘共享单倍型(H11),八宝河滩的棱果沙棘共10个个体中有7个与中国沙棘共享一个单倍型(H4),3个与肋果沙棘共享单倍型(H7).应用最大简约法(MP)分别对两地区的棱果沙棘及其亲本trnS-G序列构建的系统发育树中棱果沙棘的大部分个体都与中国沙棘聚在一起,另外,棱果沙棘4种特有单倍型(H3、H7、H8、H9)的6个个体在系统树上也与中国沙棘聚为一支.以上结果进一步证明了二倍体自然杂交种棱果沙棘为双向杂交起源,但其主要母本来源应为中国沙棘.

  5. 沙棘原汁澄清处理对沙棘酒香气成分的影响%Effect of sea-buckthorn original juice clarification on the aroma components of wine

    Institute of Scientific and Technical Information of China (English)

    刘晓娜; 韩建春; 李玲伊

    2012-01-01

    In order to find out the influences of fruit juice clarification on formation of higher alcohols and aroma compounds of sea-buckthorn wine,pectinase was utilized to clarify sea-buckthom juice. The clarified juice and original juice were regulated to sugar concentration of 23% and pH3.7,and then added active dry yeast of 0.18% and fermented at 28.5~C. The sea-buckthorn wine was detected after aging by the GC-MS and result indicated that 58 kinds of aromatic compounds were identified in sea-buckthorn wines fermented with the clarified juice and 52 aromatic compounds with the original juice. 28 kinds of aromatic compounds were different from each other,which resulted in the difference of aroma distinctively. 3-methyl-1 -butanol and octylic acid ethyl ester were the major aroma components of sea-buckthorn wines and their relative contents were 31.24% and 10.40% in wine made by clarified juice respectively and increased by 16.45% and 5.32% over sea-buckthorn wine made by original juice. Clarification before fermentation was favorable to form aroma of sea-buckthorn wine.%为研究沙棘原汁澄清处理对沙棘酒发酵速度和香气成分形成的影响,利用果胶酶对沙棘原汁进行澄清处理。分别对沙棘澄清汁和未处理的沙棘原汁调整到糖度23%、pH3.7,然后接入0.18%的活性干酵母于28.5℃下发酵,陈酿后采用气-质联用仪(GC-MS)对各发酵酒进行香气成分检测。结果表明:以澄清汁为原料发酵的沙棘酒中检测出58种香气化合物;原汁发酵的沙棘酒中检测出52种香气成分,其中有28种非两者共有,产生了非常明显的香气差异。主要香气成分为3-甲基-1-丁醇和辛酸乙酯,用澄清汁发酵的沙棘酒其相对含量分别为31.24%和10.40%,较原汁发酵的沙棘酒分别提高了16.45%和5.32%。因此表明对沙棘汁澄清处理更有利于沙棘酒香气的形成。

  6. Physicochemical properties changes of sea buckthorn cloudy juice during cold crushing, concentrating and storage%加工过程中冷打浆沙棘浓缩浊汁理化特性的变化

    Institute of Scientific and Technical Information of China (English)

    宋自娟; 徐怀德; 高锦明; 张明兰; 肖蕊; 李文娟

    2014-01-01

    Sea buckthorn berries were used as raw materials to study the process of concentrating sea buckthorn cloudy juice and their quality. Physicochemical properties including vitamin C, total flavonoids, total polyphenols, polysaccharides, pH value, total acidity, and browning index changed. Cold crushing sea buckthorn berries, concentrating production and the storage of concentrated cloudy juices were investigated systematically. The results showed that the quality of sea buckthorn juice obtained by cold crushing process technology was excellent. The contents of soluble solids and total acidity reached to 18.0% and 4.24% of sea buckthorn juice obtained respectively. It contained vitamin C 729.69 mg/100mL, flavonoids 445.17 mg/100mL, total polyphenols 924.67 mg/100mL and polysaccharides 25.22 mg/100mL. Disc milk separator removed the impurities and oil in sea buckthorn juice due to horizontal screw centrifuge. Whole cold crushing was fast with low temperature. Nutrient and health components in sea buckthorn juice lost less, they had been effectively enriched. Then, different concentrations for multiple sea buckthorn juices were processed at 45℃ by evaporation in the vacuum of 0.095~0.1 MPa. With the increase of the concentration, the loss rate of nutrients and browning index became larger, the total acidity and 5-HMF content were increased and pH value was decreased. Different concentratedn juices were placed at room temperature (10 to 25℃) and refrigerated conditions (5 to 8℃) for 60 days, their physicochemical properties were changed as follows. Vitamin C, flavonoids, total polyphenols and polysaccharide contents decreased gradually, pH and total acidity did not change significantly, browning index and 5-HMF content increased. Furthermore, refrigerated conditions were conducive to the preservation of the sea buckthorn juice compared with the room temperature condition. The nutrient and health components in preserving rate of same multiple sea buckthorn cloudy

  7. Electrochemical determination of serotonin in oral fiquid of fructus hippophae%沙棘口服液中5-羟色胺的电化学检测

    Institute of Scientific and Technical Information of China (English)

    任旺; 李敏娇; 张英

    2012-01-01

    采用电化学沉积法制备咖啡酸膜修饰电极,研究了5-羟色胺(5-HT)在该修饰电极上的电化学行为.结果表明,高浓度的抗坏血酸(AA)和肾上腺素(EP)共存不干扰5-HT的检测,5-HT在0.41 V和-0.12 V出现一对灵敏的氧化还原峰,其氧化峰电流与浓度在2.0 ×10-6 ~6.0×10-5 mol/L范围内呈良好的线性关系,检测下限为8.0×10-7 mol/L.该修饰电极制备简单,可准确检测神兴沙棘欣之安口服液中5-HT的含量.%Caffeic acid-modified electrode was constructed by potentiostatic technique. The electrochemical behavior of serotonin (S-HT) at modified electrode has been investigated by cyclic voltammetry ( CV ). The proposed electrode exhibits a promotion effect on the oxidation of 5-HT, and the interferences of ascorbic acid and epinephrine for determination of 5-HT have been investigated. The oxidative peak current obtained (ipn) is linearly dependent on the 5-HT concentrations in the range of 2.0 x 10 -16~6. 0 x 10-5 mol/L with the detection limit of 8. 0 x 10-7 mol/L. This sensor was further applied for the determination of 5-HT in Chinese traditional drug Oral Liquid of Fructus Hippophae.

  8. The Impact on Crude Polysaccharide Extraction of Seed and Peels Dregs of Hippophae with Different Broken Method%破壁方法对沙棘籽及果渣粗多糖提取率的影响

    Institute of Scientific and Technical Information of China (English)

    陈丽娜; 吴琼; 邹险峰; 张莉宏

    2014-01-01

    With seed and peels dregs of hippophae as material, polysaccharide was dissolved fully by breaking the cytoderm. Four methods with enzymatic, cold solvent, microwave and ultrasonic were compared to the amount of assessment indicators for Polysaccharide dissolved. The results showed that the best method in breaking the cytoderm was microwave treatment and the best conditions was:power 600 W;5 min/time broken;two times;interval 3 min, Under this condition, crude polysaccharide extraction rate of seed and peels dregs of hippophae was 1.892%.%以沙棘榨汁提油后的籽及果渣为原料,经过破壁手段而使多糖充分溶出。本试验采用生物酶法、冻溶、微波及超声波四种方法进行对比破壁,以粗多糖提取率为考核指标,确定了最佳细胞破壁方法为微波破碎,并且确定微波破碎工艺条件为:功率600 W,破碎5 min/次,破碎次数为2次,间歇3 min,在此条件下沙棘籽及果皮渣中粗多糖提取率为1.892%。

  9. 复方沙棘片的制备和质量标准研究%Study on Preparation and Quality Standard of Compound Sea Buckthorn Tablets

    Institute of Scientific and Technical Information of China (English)

    王小宁; 杨黎彬; 张存劳

    2016-01-01

    To optimize the preparation and prescription of tablets and study the quality standards of sea buck-thorn tablets. Used mesh size, drying time and drying temperature as factors to determine the best preparation process,and used the different kinds of fillers, adhesives, lubricants as factors to optimize the prescription.The total flavonoids and proanthocyanidins were identified by TLC and determined by UV-visible spectrophotome-try. Use a mixture of starch and dextrin (2 ∶ 1) as the filler,60 % ethanol as binder and 0.5 % magnesium stearate as lubricant.Granulate by 16 mesh sieve with 50℃as drying temperature and 60 min as drying time.The identification method was exclusive.The TLC sports were fairly clear.The determination of flavonoids and proan-thocyanidins kept the good linear relation in the concentration ranges of 0.01 mg/mL~0.05 mg/mL (r=0.999 0), 0.01 mg/mL~0.05 mg/mL (r=0.999 5). The average recoveries of flavonoids and proanthocyanidins were 100.36 %and 98.53%respectively. The compound sea buckthorn tablets have good appearance, hardness and disintegrate fast. The qualitative and quantitative analysis method is simple and accurate.It can effectively con-trol the quality of the tablet.%优化复方沙棘片的制备工艺和处方,研究制定复方沙棘片的质量标准。以颗粒性状、片剂的外观、硬度和崩解时限为指标,确定最优制备工艺和处方;采用薄层色谱鉴别法对复方沙棘片中总黄酮和原花青素进行定性鉴别;采用紫外-可见分光光度法对有效成分总黄酮和原花青素进行定量测定。结果表明,复方沙棘片的最优处方和制备工艺为:以淀粉和糊精(2∶1)的混合物为稀释剂,60%乙醇为黏合剂,0.5%硬脂酸镁为润滑剂,用16目筛湿法制粒,50℃下干燥60 min,整粒后压片。薄层色谱鉴别法专属性强,黄酮、原花青素的斑点清晰,紫外-可见分光光度法测定沙棘总黄酮和葡萄籽原花青

  10. The multifunctionality of berries toward blood platelets and the role of berry phenolics in cardiovascular disorders.

    Science.gov (United States)

    Olas, Beata

    2016-10-25

    Diet and nutrition have an important influence on the prophylaxis and progression of cardiovascular disease; one example is the inhibition of blood platelet functions by specific components of fruits and vegetables. Garlic, onion, ginger, dark chocolate and polyunsaturated fatty acids all reduce blood platelet aggregation. A number of fruits contain a range of cardioprotective antioxidants and vitamins, together with a large number of non-nutrient phytochemicals such as phenolic compounds, which may possess both antioxidant properties and anti-platelet activity. Fresh berries and berry extracts possess high concentrations of phenolic compounds, i.e. phenolic acid, stilbenoids, flavonoids and lignans. The aim of this review article is to provide an overview of current knowledge of the anti-platelet activity of berries, which form an integral part of the human diet. It describes the effects of phenolic compounds present in a number of berries, i.e. black chokeberries - aronia berries (Aronia melanocarpa), blueberries (Vaccinium myrtillus), cranberries (Vaccinium sect. Oxycoccus), sea buckthorn berries (Hippophae rhamnoides) and grapes (Vitis), as well as various commercial products from berries (i.e. juices), on platelets and underlying mechanisms. Studies show that the effects of berries on platelet activity are dependent on not only the concentrations of the phenolic compounds in the berries or the class of phenolic compounds, but also the types of berry and the form (fresh berry, juice or medicinal product). Different results indicate that berries may play a role in the prevention of cardiovascular disorders, but the development of well-controlled clinical studies with berries is encouraged.

  11. Isorhamnetin inhibits H₂O₂-induced activation of the intrinsic apoptotic pathway in H9c2 cardiomyocytes through scavenging reactive oxygen species and ERK inactivation.

    Science.gov (United States)

    Sun, Bing; Sun, Gui-Bo; Xiao, Jing; Chen, Rong-Chang; Wang, Xin; Wu, Ying; Cao, Li; Yang, Zhi-Hong; Sun, Xiao-Bo

    2012-02-01

    As a traditional Chinese medicine, the sea buckthorn (Hippophae rhamnoides L.) has a long history in the treatment of ischemic heart disease and circulatory disorders. However, the active compounds responsible for and the underlying mechanisms of these effects are not fully understood. In this article, isorhamnetin pretreatment counteracted H(2)O(2)-induced apoptotic damage in H9c2 cardiomyocytes. Isorhamnetin did not inhibit the death receptor-dependent or extrinsic apoptotic pathways, as characterized by its absence in both caspase-8 inactivation and tBid downregulation along with unchanged Fas and TNFR1 mRNA levels. Instead, isorhamnetin specifically suppressed the mitochondria-dependent or intrinsic apoptotic pathways, as characterized by inactivation of caspase-9 and -3, maintenance of the mitochondrial membrane potential (ΔΨm), and regulation of a series of Bcl-2 family genes upstream of ΔΨm. The anti-apoptotic effects of isorhamnetin were linked to decreased ROS generation. H(2)O(2) activated ERK and p53, whereas isorhamnetin inhibited their activation. ERK overexpression overrode the isorhamnetin-induced inhibition of the intrinsic apoptotic pathway in H9c2 cardiomyocytes, which indicated that an ERK-dependent pathway was involved. Furthermore, N-acetyl cysteine (a potent ROS scavenger) could attenuate the H(2)O(2)-induced apoptosis. However, PD98059 (an ERK-specific inhibitor) could not effectively antagonize ROS generation, which indicates that ROS may be an upstream inducer of ERK. In conclusion, isorhamnetin inhibits the H(2)O(2)-induced activation of the intrinsic apoptotic pathway via ROS scavenging and ERK inactivation. Therefore, isorhamnetin is a promising reagent for the treatment of ROS-induced cardiomyopathy.

  12. Differences in hydrological responses for different vegetation types on a steep slope on the Loess Plateau, China

    Science.gov (United States)

    Duan, Liangxia; Huang, Mingbin; Zhang, Luodan

    2016-06-01

    Extensive vegetation restoration practices have been implemented to control soil erosion on the Loess Plateau, China. However, no strict guidelines are available to determine the most suitable plant species for vegetation restoration within a given area. The objective of this study was to quantify the changes of each component (soil water storage, surface runoff, and actual evapotranspiration) of a water balance model and soil loss over time under eight different vegetation types, and to further determine the optimal vegetation type for soil and water conservation and sustainable ecological restoration on the steep slopes (>25°) on the Loess Plateau. The results indicated that vegetation type substantially affected soil water storage and that the greatest soil water storage in both the shallow (0-2 m) and the deep soil layers (2-5 m) occurred under Bothriochloa ischaemum L. (BOI). Vegetation type also affected surface runoff and soil losses. The most effective vegetation types for reducing soil erosion were BOI and Sea-buckthorn (Hippophae rhamnoides L.), while Chinese pine (Pinus tabulaeformis Carr.) and Chinese pine + Black locust (Robinia pseudoacacia L.) were the most ineffective types. Soil water dynamics and evapotranspiration varied considerably among the different vegetation types. A soil water surplus was only found under BOI, while insufficient water replenishment existed under the other seven vegetation types. The higher water consumption rates of the seven vegetation types could result in soil desiccation, which could lead to severe water stresses that would adversely affect plant growth. This study suggested that both vegetation type and its effect on controlling soil erosion should be considered when implementing vegetation restoration and that BOI should be highly recommended for vegetation restoration on the steep slopes of the Loess Plateau. A similar approach to the one used in this study could be applied to other regions of the world confronted

  13. Berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease.

    Science.gov (United States)

    Seeram, Navindra P

    2008-02-13

    An overwhelming body of research has now firmly established that the dietary intake of berry fruits has a positive and profound impact on human health, performance, and disease. Berry fruits, which are commercially cultivated and commonly consumed in fresh and processed forms in North America, include blackberry ( Rubus spp.), black raspberry ( Rubus occidentalis), blueberry ( Vaccinium corymbosum), cranberry (i.e., the American cranberry, Vaccinium macrocarpon, distinct from the European cranberry, V. oxycoccus), red raspberry ( Rubus idaeus) and strawberry ( Fragaria x ananassa). Other berry fruits, which are lesser known but consumed in the traditional diets of North American tribal communities, include chokecherry ( Prunus virginiana), highbush cranberry ( Viburnum trilobum), serviceberry ( Amelanchier alnifolia), and silver buffaloberry ( Shepherdia argentea). In addition, berry fruits such as arctic bramble ( Rubus articus), bilberries ( Vaccinuim myrtillus; also known as bog whortleberries), black currant ( Ribes nigrum), boysenberries ( Rubus spp.), cloudberries ( Rubus chamaemorus), crowberries ( Empetrum nigrum, E. hermaphroditum), elderberries ( Sambucus spp.), gooseberry ( Ribes uva-crispa), lingonberries ( Vaccinium vitis-idaea), loganberry ( Rubus loganobaccus), marionberries ( Rubus spp.), Rowan berries ( Sorbus spp.), and sea buckthorn ( Hippophae rhamnoides), are also popularly consumed in other parts of the world. Recently, there has also been a surge in the consumption of exotic "berry-type" fruits such as the pomegranate ( Punica granatum), goji berries ( Lycium barbarum; also known as wolfberry), mangosteen ( Garcinia mangostana), the Brazilian açaí berry ( Euterpe oleraceae), and the Chilean maqui berry ( Aristotelia chilensis). Given the wide consumption of berry fruits and their potential impact on human health and disease, conferences and symposia that target the latest scientific research (and, of equal importance, the dissemination of

  14. 沙棘果醋发酵过程中醋酸发酵条件的优化研究%Optimization of acetic acid fermentation conditions of sea buckthorn fruit vinegar

    Institute of Scientific and Technical Information of China (English)

    牛广财; 朱丹; 魏文毅; 王永苓; 关琛

    2012-01-01

    在初始酒精度、初始pH值、装液量、摇床转数等因素的单因素试验基础上,采用Box-Behnken试验设计,通过响应面分析,对沙棘果醋的醋酸发酵工艺条件进行优化.结果表明,沙棘果醋的醋酸发酵最佳工艺条件为初始酒精度5.5%vol、pH值为4.0、装液量96mL/500mL、摇床转数125r/min,在此条件下的验证试验表明,沙棘果醋的醋酸转化率为98.75%.%Based on the single factors including the initial alcohol content, the initial pH, volume of shaking flask and rotation speed, Box-Behnken experiment design was used to optimize acetic acid fermentation conditions of sea buckthorn fruit vinegar. The optimal conditions were as follows: the initial alcohol content 5.5%vol, the initial pH value 4.0, the volume of shaking flask 96ml of 500ml and the rotation speed 125r/min. Under the optimal conditicns, the vinegar conversion rate was 98.75%.

  15. 沙棘果渣栽培木耳生产工艺及产品营养成分分析%Production Process for Cultivating Auricularia auricula-judae with Marc of Sea Buckthorn Fruit and Nutritional Composition of Product

    Institute of Scientific and Technical Information of China (English)

    张丕奇; 刘佳宁; 孔祥辉

    2011-01-01

    A study was conducted to cultivate Auricul aria auricula-judae with marc of sea buckthorn fruit in order to reuse the marc and solve the problem of shortage of raw materials for A. auricula-judae cultivation. Marc of sea buckthorn fruit was supplemented to the cultivation materials in a certain proportion. The optimum additive amount and formula were determined by orthogonal experiment. Effects of this formula on the mycelium growth of two strains of A. auricula-judae were studied, and the optimal cultivation process was determined. The main nutritional composition of the cultivated A. auricula-judae was analyzed. Results indicate that adding 20% of marc of sea buckthorn fruit to the cultivation materials can promote the mycelium growth and obviously increase the yield of A. auricula-judae. The crude protein content in the products with good taste and thickness increased by 3%, and other nutrients changed little. It is stable and feasible to cultivate A.auricula-judae using the marc of sea buckthorn fruit.%为了使废弃的沙棘果渣得到再利用,解决木耳栽培原料短缺的问题,将沙棘果渣按照一定比例添加到木耳栽培料中,通过正交试验确定了沙棘果渣最佳添加量(20%沙棘果渣)和最佳配方,并研究了该配方对不同木耳品种(黑29、黑威11号)菌丝生长的影响,通过出耳试验确定了栽培工艺,测定了所生产木耳的主要营养成分.结果表明:添加20%的沙棘果渣对黑29、黑威11号两个木耳品种的菌丝生长均有促进作用,木耳产量明显增加,木耳片厚、口感好,粗蛋白增加3%,其他营养成分没有明显变化.利用沙棘果渣栽培木耳生产工艺稳定,可行性强.

  16. 沙棘叶总黄酮含量测定方法的建立%Establishment of determination method of total flavonoids in H ip pophe rhamnoids L leaves

    Institute of Scientific and Technical Information of China (English)

    李淑珍; 武飞; 陈月林; 杨颖

    2015-01-01

    A method for determination of total flavonoids in Hippophe rhamnoids L leaves is established , by singlefactor screening , multiple‐factor orthogonal test and methodology examination of NaNO2‐Al (NO3 )3‐NaOH system spectrophotometry . The single factor includs reaction volume and reaction time .The results show that the optimization methods are :Take 1 mL sample solution in the beaker ,add 70% ethanol 1 mL and 5% NaNO2 0.5 mL ,shake well ,react 6 min .Then add 10% Al(NO3)3 0.5 mL , shake well , react 6 min ;After that add 4% NaOH 6 mL , shake well , react 15 min , determine absorbance at 510 nm with 70% ethanol as reference solution . The methodology shows that the method has good stability and reproducibility , high precision and recovery . It is more suitable for the determination content of total flavonoids in Hippophe rhamnoids L leaves ,which has simple equipment , convenient operation and high accurate . using this method , the content of total flavonoids is 2.049% , which is 1.337 times than before optimized . Both of then reach to the regulation standard of “China pharmacopoeia” . This method can be looked as a reference method for the determination of total flavonoids in other materials .%本研究通过比色条件NaNO2,Al(NO3)3和NaO H的加入量和静置时间单因子筛选、多因子正交试验和方法学考察,建立了NaNO2‐Al(NO3)3‐NaO H体系分光光度法测定沙棘叶总黄酮含量的优化方法.结果得出测定沙棘叶总黄酮含量的步骤是:取1 mL样液于烧杯中,加70%的乙醇1 mL ,再加入5%的亚硝酸钠溶液0.5 mL ,摇匀,静置6 min ;再加入10%的硝酸铝溶液0.5 mL ,摇匀,静置6 min;然后加入4%的氢氧化钠溶液6 mL ,摇匀,放置15 min后,以70%乙醇作参比溶液,于波长510 nm 处测定吸光值.测得沙棘总黄酮含量为2.049%,比优化前提高1.337倍,均达到《中国药典》规定的标准.该法稳定性和重现

  17. 均匀设计优选提取条件测定沙棘粉中儿茶素含量%Determination of uniform design of extraction conditionsfor sea buckthorn powder catechin content

    Institute of Scientific and Technical Information of China (English)

    姚志敏; 陈秀红; 林童; 魏玉海

    2015-01-01

    Objective:Screening methods to optimize the extraction of catechins sea buckthorn powder,and determine its content by HPLC. Methods:With uniform design to optimize ultrasonic extraction conditions,using Agilent ZORBAX SB-C18 column with methanol - acetic acid solution(pH value adjusted to 3.2 with acetic acid)(25:75)as the mobile phase, flow rate 1 mL / min;column temperature was 25 ℃ The detection wavelength 280nm. Results:The optimum extraction con⁃ditions optimized concentration of 74.0% for methanol,water bath temperature of 70 ℃,ultrasonic time of 30min. Catechin at 0.50 ~ 10.0μg / mL concentration range good linearity,the correlation coefficient(r)was 0.9999,the average recovery was 97.25%(RSD=1.77%). Conclusion:Method for the determination of the trial established simple,reproducible and has a strong specificity.%目的:筛选优化沙棘粉中儿茶素的提取方法,并用HPLC测定其含量。方法:以均匀设计法优化提取条件,采用Agilent ZORBAX SB-C18柱,以甲醇—乙酸溶液(用乙酸调pH值至3.2)(25:75)为流动相,流速1 mL/min;柱温25℃,检测波长280nm。结果:优选出的最佳提取条件:甲醇浓度为74.0%,水浴温度为70℃,超声时间为30min。儿茶素在0.50~10.0μg/mL 浓度范围内线性良好,相关系数(r)为0.9999,平均回收率为97.25%(RSD=1.77%)。结论:本试验建立的含量测定方法操作简便、重现性好且具有较强的专属性。

  18. Antimicrobial Effect of Escherichia Coli on Essential Oils Derived from Romanian Aromatic Plants

    Directory of Open Access Journals (Sweden)

    Şandru Daniela Maria

    2015-07-01

    Full Text Available This paper investigates the antimicrobial action of Escherichia coli ATCCR CRM-8739TM on the following essential oils: Teucrium marum, Pinus sylwestris, Thymus vulgaris, Salviae aethedaroleum, Cinnamomum aromaticum, Hippophae rhamnoides, Lavandula angustifolia, Abies alba, Zingiber officinale, Anethum graveolens, Coriandrum sativum, Origanum vulgare, extracted industrialy from romanian plants, using the diffusion disc method. The most intense activity was observed at the essential oil of Cinnamomum aromaticum (cinnamon and the mildest activity was observed at Zingiber officinale (ginger. Many of the essential oils tested exhibited moderate antimicrobial activity, as Teucrium marum, Thymus vulgaris, Hippophae rhamnoides, Lavandula angustifolia,Coriandrum sativum. The lowest antibacterial activity was exhibited on Pinus sylwestris, Salviae aethedaroleum, Zingiber officinale and Anethum graveolens.

  19. Eco-toxicological effects of two kinds of lead compounds on forest tree seed in alkaline soil.

    Science.gov (United States)

    Yang, Nan; Zhou, Fu-Rong; Wang, Jin-Xin

    2016-03-01

    In order to compare the different eco-toxicological effects of lead nitrate and lead acetate on forest tree seed, a biological incubation experiment was conducted to testify the inhibition effects of two lead compounds on rates of seed germination, root and stem elongation, and seedling fresh weight for six plants (Amaorpha fruticosa L., Robinia psedoacacia L., Pinus tabuliformis Carr., Platycladus orientalis L., Koelreuteria paniculata Laxm., Hippophae rhamnoides L.) in soil. The results indicate that the inhibition effects of the two lead compounds on the rates of root elongation of plants were greater than other indices; root elongation can possibly be used as indices to investigate the relationship between lead toxicity and plant response. The response of trees to lead toxicity varied significantly, and the order of tolerance to lead pollution was as follows: Amaorpha fruticosa L. > Platycladus orientalis L. > Koelreuteria paniculata Laxm. > Robinia psedoacacia L. > Pinus tabuliformis Carr. > Hippophae rhamnoides L. Therefore, we suggest that Amaorpha fruticosa L. and Platycladus orientalis L. be used as tolerant plants for soil phytoremediation and Hippophae rhamnoides L. as an indicative plant to diagnose the toxicity of lead pollution on soil quality. Lead nitrate and lead acetate differentially restrain seeds, with seeds being more sensitive to lead nitrate than lead acetate in the soil. Thus, the characteristics of lead compounds should be taken into full consideration to appraise its impact on the environment.

  20. Identification of the traditional Tibetan medicine "Shaji" and their different extracts through tri-step infrared spectroscopy

    Science.gov (United States)

    Liu, Yue; Li, Jingyi; Fan, Gang; Sun, Suqin; Zhang, Yuxin; Zhang, Yi; Tu, Ya

    2016-11-01

    Hippophae rhamnoides subsp. sinensis Rousi, Hippophae gyantsensis (Rousi) Y. S. Lian, Hippophae neurocarpa S. W. Liu & T. N. He and Hippophae tibetana Schlechtendal are typically used under one name "Shaji", to treat cardiovascular diseases and lung disorders in Tibetan medicine (TM). A complete set of infrared (IR) macro-fingerprints of these four Hippophae species should be characterized and compared simply, accurately, and in detail for identification. In the present study, tri-step IR spectroscopy, which included Fourier transform IR (FT-IR) spectroscopy, second derivative IR (SD-IR) spectroscopy and two-dimensional correlation IR (2D-IR) spectroscopy, was employed to discriminate the four Hippophae species and their corresponding extracts using different solvents. The relevant spectra exhibited the holistic chemical compositions and variations. Flavonoids, fatty acids and sugars were found to be the main chemical components. Characteristic peak positions, intensities and shapes derived from FT-IR, SD-IR and 2D-IR spectra provided valuable information for sample discrimination. Principal component analysis (PCA) of spectral differences was performed to illustrate the objective identification. Results showed that the species and their extracts can be clearly distinguished. Thus, a quick, precise and effective tri-step IR spectroscopy combined with PCA can be applied to identify and discriminate medicinal materials and their extracts in TM research.

  1. Genetic diversity and genetic structure of the northern margin populations of Hippophae neurocarpa%肋果沙棘北缘居群的遗传多样性与遗传结构

    Institute of Scientific and Technical Information of China (English)

    李霓; 蒋严妃; 苏雪; 陈纹; 张辉; 孙坤

    2016-01-01

    We analyzed five natural populations of Hippophae neurocarpa in the Qilian area of Qinghai Province with SSR molecular markers, in order to understand the genetic diversity of the northern margin populations of H. neurocar-pa on the small geographic scales and the genetic structure of the populations which distribute fragmentally, and to pro-vide reference for resource protection of H. neurocarpa populations. We used six microsatellite primers to amplify the DNA of 107 samples and detected 27 alleles, with the range of 2-9 and a mean value of 4.67 per locus. The average observed heterozygosity ( Ho) and expected heterozygosity ( He) of H. neurocarpa populations was 0.142 and 0.230 re-spectively, and the range Shannon information index (I) was between 0.280-0.567 with a average value of 0.374. The results showed that the genetic diversity of the northern margin populations of H. neurocarpa was abundant. The coeffi-cient of genetic differentiation ( Fst) was 0.483. Molecular variance analysis ( AMOVA) indicated that 48.33% of the variation existed among the populations, and 51.67%existed within the populations. No significant correlation between genetic distance and geographic distance was detected by Mental test and the detection of gene flow found that Nm was only 0.328 among populations. These results suggested that genetic drift was one of the key factors for the differentia-tion of populations. The 5 populations were divided into 2 groups based on the structure analysis. UPGMA clustering showed that the 5 populations were clustered into 2 branches, one of which was ARX, which was consistent with the results of the principal coordinate analysis. According to the analysis of the genetic structure, it is suggested to protect populations in situ as many as possible.%该研究利用SSR分子标记,对肋果沙棘( Hippophae neurocarpa)分布区北缘青海祁连地区5个自然居群进行分析,旨在了解小地理尺度下肋果沙棘北缘居群的遗传多样

  2. Nutritional profile of phytococktail from trans-Himalayan plants.

    Directory of Open Access Journals (Sweden)

    Priyanka Dhar

    Full Text Available We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides, apricot (Prunus armeniaca, and roseroot (Rhodiola imbricata from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS. Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID with fatty acid methyl esters (FAMEs derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs, palmitoleic acid (43.47%, oleic acid (20.89%, and linoleic acid (4.31% were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES, and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit

  3. 中国沙棘克隆生长对不同光照强度的响应%The Clonal Growth of Hippophae rhamniodes L.subsp.sinensis in Response to Different Light Intensity

    Institute of Scientific and Technical Information of China (English)

    唐翠平; 郭峰; 徐德兵; 贺斌; 李甜江; 李根前

    2014-01-01

    The clonal growth of Hippophae rhamniodes L.subsp.sinensis in response to different light intensity (100%, 70 %, 50%and 20%of nature light ) by using shading net was studied , and the results showed: 1 ) The pop-ulation growth, the biomass accumulation and its distribution , underground clonal growth parameters , and ect. were all showed a change of quadratic parabolic curve with the light intenstity increasing from 20%to 100%, and the best light intenstity for clonal growth was 50%~70%.2 ) The optimum light intensity for population growth , biomass of community and different organs , daughter ramet number and spacer growth were 48.0 %~61.7 %, 52.8 %~53.6 %, and 55.6%~57.6% respectively , and there was a cooperative function and a trade-off re-lation among them .3 ) At the optimum light intenstity , the population was characterised by individual tall and concentrated distribution , and it growth partern tended to a phalanx type .For too much or too small light intensi-ty, the population was characterized by individual low and dispersed distribution , and its growth pattern tended to a guerrilla type .The population growth patterns would change from guerrilla type to phalanx type , and then to guerrilla type , and formed a foraging strategies continuum with the increasing of light intensity .%利用遮阳网产生的不同光照强度(100%、70%、50%和20%的自然光照),研究了中国沙棘克隆生长对不同光照强度的响应规律。结果表明,(1)当光照强度从20%逐渐增加到100%时,种群生长量、生物量积累与分配、地下克隆器官参数等增幅均呈二次抛物线变化,相对光照强度为50%~70%是其克隆生长的最佳光照强度。(2)种群生长量、种群及各器官生物量、子株数量及萌蘖根生长量增幅最大时的光强分别为48.0%~61.7%、52.8%~53.6%、55.6%~57.6%,且分株生长量和种群生物量、萌蘖根生长量和子株数量之间

  4. 三波长HPLC同时测定沙棘颗粒中的槲皮素、山萘酚与异鼠李素%Simultaneous quantitative determination of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids granules under tri-wave length by RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    甘盛; 韩婷; 施晓光; 吴超权

    2013-01-01

    目的 用HPLC法在3个波长段同时测定沙棘颗粒中槲皮素、山萘酚与异鼠李素的含量.方法 经室温两步提取处理沙棘颗粒中的黄酮类化合物,测定其中的槲皮素、山萘酚与异鼠李素.在反相液相系统中,采用Symmetry C18柱(250 mm×4.6mm,5.0 μm),流动相为乙腈-0.4%磷酸溶液(40∶60),室温,流速1.0 mL· min-1.槲皮素、山萘酚、异鼠李素的检测波长分别为266、360、370 nm.结果 槲皮素、山萘酚与异鼠李素均在0.2~1.6 μg与峰面积线性关系良好,平均回收率分别为99.7%、98.3%、101.0%,r分别为0.9993、0.9996、0.9991.结论 所用方法灵敏度高、专属性强,适用于测定复方沙棘颗粒中的槲皮素、山萘酚与异鼠李素的含量.%OBJECTIVE To assay the content of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids Linn. granules under tri - wave length by HPLC. METHODS Flavone glycosides were extracted in two steps from hippohae rhamnoids Linn. granules , in which the quercetin, kaempferol and isorhamnetin content were assayed. The RP - HPLC workstation equipped with the Symmetry C18 column (250 mm ×4. 6 mm, 5. 0 μm) at room temperature was used, the mobile phase was composed of acetotrile and 0. 4% of phosphoric acid (4 : 6) with a flow rate of 1. 0 mL·min-1. Quercetin was detected at 266 nm, while kaempferol at 360 nm and isorhamnetin at 370 nm. RESULTS The good linearity was obtained at the concentration of 0. 2 - 1. 6 μg, the average recoveries were 99. 7% ,98. 3% and 101.0% , with the relative coefficients of 0. 9991,0. 9994 and 0. 9990, respectively. CONCLUSION The method is proved to be sensitive and specific, applicable for the determination of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids Linn, granules.

  5. [Root anatomical structure and hydraulic traits of three typical shrubs on the sandy lands of northern Shaanxi Province, China].

    Science.gov (United States)

    Ai, Shao-shui; Li, Yang-yang; Chen, Jia-cun; Chen, Wei-yue

    2015-11-01

    Root xylem anatomical structure and hydraulic traits of three typical shrubs, i.e., Salix psammophila, Caragana korshinskii and Hippophae rhamnoides, within two soil layers (0-20 cm and 30-50 cm) were compared. The results showed that S. psammophila had a higher leaf water potential than C. korshinskii and H. rhamnoides, the average maximum and minimum lumen diameter (d(max) and d(min), respectively), the average lumen area of vessels (Alum) and the ratio of lumen area of all vessels to xylem area (Aves/Axyl) in S. psammophila roots were also significantly higher than those in C. korshinskii and H. rhamnoides, and the root vessel density (VD) in S. psammophila was the same as that in H. rhamnoides but significantly higher than that in C. korshinskii. Root hydraulic conductivity in S. psammophila was 5 times of C. korshinskii and 2.8 times of H. hamnoides. The vulnerability index in S. psammophila roots was similar to that in C. korshinskii but higher than that in H. hamnoides. S. psammophila belonged to a water-spending species, whereas both C. korshinskii and H. rhamnoides were water-saving species, and C. korshinskii was more drought-resistant than H. rhamnoides. There was no difference of d(max), d(min) and Alum between roots in two soil layers, but roots within in the 30-50 cm soil layer had larger VD and Aves/Axyl. The root specific hydraulic conductivity within the 30-50 cm soil layer was significantly higher than within the surface soil layer, whereas the vulnerability index within the 30-50 cm soil layer was smaller, indicating roots in deep soil layers had higher hydraulic transport efficiency and lower hydraulic vulnerability.

  6. [Water and soil conservation function of typical plantation forest ecosystems in semi-arid region of Western Liaoning Province].

    Science.gov (United States)

    Jiang, Ping; Guo, Fang; Luo, Yue-Chu; Wei, Jing; Sun, Xiao-Wei; Wu, Gang

    2007-12-01

    From the aspects of surface runoff and soil erosion, this paper quantitatively studied the water and soil conservation function of five plantation forest ecosystems in semi-arid region of Western Liaoning Province. The results showed that various types of test plantation forest ecosystems were all able to reduce surface runoff and soil erosion effectively. In June - September, the monthly mean surface runoff coefficient of Pinus tabulaeformis forest ecosystem, P. tabulaeformis - Hippophae rhamnoides forest ecosystem, H. rhamnoides forest ecosystem, P. simonii forest ecosystem, and P. simonii - H. rhamnoides forest ecosystem was 10.1%, 6.5%, 2.3%, 8.6% and 5.3% of that of barren hill, respectively, and the soil erosion quantity was 2.65%, 0.96%, 0.15%, 2.32% and 0.69% of that of barren hill, respectively. Among the five forest ecosystems, H. rhamnoides forest ecosystem had the least surface runoff and soil erosion, being the best in water and soil conservation function.

  7. 醋柳黄酮治疗高血压的研究进展

    Institute of Scientific and Technical Information of China (English)

    高天林

    2011-01-01

    @@ 醋柳黄酮(total flavones of hippophae rhamnoides L,TFH)是一种从野生胡颓子科植物醋柳(俗称沙棘)的果实中提取出的一种有效成分.TFH含有7种单体成分,其中以槲皮素(quercetin,Que)及异鼠李素(isorhamnetin,Iso)含量较高.目前,从沙棘中提取的黄酮类化合物-TFH已广泛应用于临床.

  8. Study on Biodiversity Index of Different Models of Conversion of Farmland to Forest in Huangshui River of Qinghai Province%青海湟水河流域不同退耕还林模式生物多样性研究

    Institute of Scientific and Technical Information of China (English)

    张学元

    2011-01-01

    基于香农-威纳多样性指教,研究了青海湟水河流域相同林龄(8年)的不同退耕还林模式生物多样性.结果表明,湟水河流域不同退耕还林模式生物多样性为云杉桦树混交>沙棘>桦树>云杉>云杉沙棘混交>农田,草本层多样性>乔木层.%Based on biodiversity index of Shannon-Weiner, the effects of biodiversity index from different models of conversion of farmland to forest in Huangshui river of Qinghai Province were studied, the results showed that mixture of picea crassifolia and birch > hippophae rham-noides > birch > picea crassifolia > mixture of picea crassifolia and hippophae rhamnoides > farmland, biodiversity index of herbaceous layer >arbor layer.

  9. Extraction and purification of the polysaccharides in Hippohaere rhamnoides L.

    Institute of Scientific and Technical Information of China (English)

    XU Yaqin; JIN Ting; YU Zeyuan; FU Hong

    2007-01-01

    The different extraction technology and purification technology of Hippohpae rhamoides polysaccharides were researched in the paper. The best method of papain extraction were obtained, the ratio of papain 2%, pH at 5.5, temperature at 45℃ and extraction time of 20 min were suitable for papain extraction. The highest content of Hippohpae rhamoides polysaccharides was 44.28 mg·g-1. The optimum process of ultrasonic extraction were obtained, namely extracted for 55 min at 480 W with the material ratio of 1:20. The highest content of Hippohpae rhamoides polysaccharides was 48.63 mg·g-1. The results showed that the ultrasonic and papain extraction together was the best method, the content was 54.30 mg·g-1. After the removing protein, pigment and dialysis. Two fraction were separated from the purified Hippohpae rhamoides by DEAE-cellulose chromatography, the main fraction was collected finally. The fraction was identified by Sepharose CL-4B gel filtration. Ultraviolet spectrometry, freeze-thawing analysis showed that fraction was purified. Its molecular weight was probably 109.4 ku.

  10. Contrasting water use pattern of introduced and native plants in an alpine desert ecosystem, Northeast Qinghai–Tibet Plateau, China

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Huawu, E-mail: wuhuawu416@163.com [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Li, Xiao-Yan, E-mail: xyli@bnu.edu.cn [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Jiang, Zhiyun; Chen, Huiying; Zhang, Cicheng; Xiao, Xiong [College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China)

    2016-01-15

    Plant water use patterns reflect the complex interactions between different functional types and environmental conditions in water-limited ecosystems. However, the mechanisms underlying the water use patterns of plants in the alpine desert of the Qinghai–Tibet Plateau remain poorly understood. This study investigated seasonal variations in the water sources of herbs (Carex moorcroftii, Astragalus adsurgens) and shrubs (Artemisia oxycephala, Hippophae rhamnoides) using stable oxygen-18 isotope methods. The results indicated that the native herbs (C. moorcroftii, A. adsurgens) and one of the shrubs (A. oxycephala) mainly relied on water from the shallow layer (0–30 cm) throughout the growing season, while the introduced shrub (H. rhamnoides) showed plasticity in switching between water from shallow and deep soil layers depending on soil water availability. All studied plants primarily depended on water from shallow soil layers early in the season. The differences of water use patterns between the introduced and native plants are closely linked with the range of active root zones when competing for water. Our findings will facilitate the mechanistic understanding of plant–soil–water relations in alpine desert ecosystems and provide information for screening introduced species for sand fixation. - Highlights: • Stable oxygen-18 in soil water experienced great evaporation enrichment. • H. rhamnoides experiences a flexible plasticity to switch between shallow and deep soil water. • Native plants mostly relied on shallow and middle soil water. • Water-use patterns by introduced-native plants are controlled by root characteristics.

  11. 矿山矸石台地植被恢复栽培模式研究%Research on Cultivation Pattern of Vegetation Recovery at Waste Heap Platform of Coal Mine

    Institute of Scientific and Technical Information of China (English)

    孙翠玲; 苏铁成; 郭玉文

    2005-01-01

    In order to study 5 cultivation models such as arbor-shrub, arbor-shrub-herb and their disposition for vegetation recovery, 10 arbor species, 7 shrub species and 5 herb species suitalde for locat sites were choiced at waste heap platform of Fushun Coal Mine. The experiment adopted the randomized block design, three replicates, with 0.067hm2 at each thinned plot. The experiment results of 2-year vegetation recovery showed that the survival rate of woody plants was significantly different among species and there existed significant differences in ground diameter and height increments. The results of the initial stage of the experiment showed that the disposition of A and C were the optimal cultivation models, and Robinia pesudoacacia, Hippophae rhamnoides, Amorpha fruticosa, Caragana chamlagu,Ulmus pumila were the best tree species for arbor-shrub-herb disposition.

  12. Evaluation of nutrient value of seabuckthorn in north China

    Institute of Scientific and Technical Information of China (English)

    Hu Jian-zhong; Guo Xiao-feng

    2006-01-01

    Five minerals or elements, crude protein, lysine, methionine+cystine, calcium and phosphorus, were selected for evaluation of the food value of seabuckthorn (Hippophae rhamnoides ssp. sinensis) using the method of "food analysis". The decision values (δi)of seabuckthorn seeds, leaves and fruit residues rank in the fifth, sixth and ninth position among the 16 types of fodder. Seeds, leaves and fruit residues of seabuckthorn are suitable for livestock and poultry fodder. The weights of livestock and poultry are considerably increased after feeding with seabuckthorn. The development of seabuckthorn should have great potentials as food, pre-food and food additives for livestock and poultry in north China. Processing facilities should pay more attention to seabuckthorn food development methods and strategies and the seabuckthorn fodder industry should be promoted.

  13. Isorhamnetin Attenuates Staphylococcus aureus-Induced Lung Cell Injury by Inhibiting Alpha-Hemolysin Expression.

    Science.gov (United States)

    Jiang, Lanxiang; Li, Hongen; Wang, Laiying; Song, Zexin; Shi, Lei; Li, Wenhua; Deng, Xuming; Wang, Jianfeng

    2016-03-01

    Staphylococcus aureus, like other gram-positive pathogens, has evolved a large repertoire of virulence factors as a powerful weapon to subvert the host immune system, among which alpha-hemolysin (Hla), a secreted pore-forming cytotoxin, plays a preeminent role. We observed a concentration-dependent reduction in Hla production by S. aureus in the presence of sub-inhibitory concentrations of isorhamnetin, a flavonoid from the fruits of Hippophae rhamnoides L., which has little antibacterial activity. We further evaluate the effect of isorhamnetin on the transcription of the Hla-encoding gene hla and RNAIII, an effector molecule in the agr system. Isorhamnetin significantly down-regulated RNAIII expression and subsequently inhibited hla transcription. In a co-culture of S. aureus and lung cells, topical isorhamnetin treatment protected against S. aureus-induced cell injury. Isorhamnetin may represent a leading compound for the development of anti-virulence drugs against S. aureus infections.

  14. 高效液相色谱法测定心达康片中槲皮素和异鼠李素的含量

    Institute of Scientific and Technical Information of China (English)

    王亚丽; 王烨; 盛惟

    2006-01-01

    心达康片为临床治疗心血管病常用药,收载在《国家药品标准》.其主要成分为沙棘黄酮(学名:醋柳黄酮total of Hippophae rhamnoides,TFH).沙棘黄酮主含槲皮素(quercetin)、异鼠李素(isorhamnetin)等黄酮苷类化合物.具有补气益心、化瘀通脉、消痰运脾之功效,用于心气虚弱、心脉瘀阻、痰湿困脾所致的心慌、心悸、心痛、气短胸闷、血脉不畅、咳累等症.

  15. Application in Supercritical Fluid Extraction of Effective Factor in Plant%超临界流体萃取在植物有效成分提取中的应用

    Institute of Scientific and Technical Information of China (English)

    刘艳

    2012-01-01

    The specificity of supercritical fluid,principle and schematic diagram of extraction are stated.The development in supercritical fluid extraction the effective factor in plan such as plan essential oil(rose essential oil,seed oil from hippophae rhamnoides),plan pigment(capsicum pigment,tomato red pigment) and factors in Chinese medicine(astragaloside,artemisinini) are reviewed.And the development of supercritical fluid extraction in future is forecasted.%文章论述了超临界流体的基本特性和萃取的原理,超临界流体在植物有效成分如植物精油、植物色素和中药有效成分等方面的研究现状,以及超临界流体萃取技术的发展趋势.

  16. Sap flow in response to rainfall pulses for two shrub species in the semiarid Chinese Loess Plateau

    Directory of Open Access Journals (Sweden)

    Jian Shengqi

    2016-06-01

    Full Text Available Rainfall pulses can significantly drive the evolution of the structure and function of semiarid ecosystems, and understanding the mechanisms that underlie the response of semiarid plants to rainfall is the key to understanding the responses of semi–arid ecosystems to global climatic change. We measured sap flow in the branches and stems of shrubs (Caragana korshinskii Kom. and Hippophae rhamnoides Linn. using sap flow gauges, and studied the response of sap flow density to rainfall pulses using the “threshold–delay” model in the Chinese Loess Plateau. The results showed that the sap flow began about 1 h earlier, and increased twofold after rainfall, compared to its pre-rainfall value. The sap flow increased significantly with increasing rainfall classes, then gradually decreased. The response of sap flow was different among rainfall, species, position (branch and stem during the pulse period, and the interactive effects also differed significantly (P < 0.0001. The response pattern followed the threshold–delay model, with lower rainfall thresholds of 5.2, 5.5 mm and 0.7, 0.8 mm of stem and branch for C. korshinskii and H. rhamnoides, demonstrating the importance of small rainfall events for plant growth and survival in semi–arid regions.

  17. Contrasting water use pattern of introduced and native plants in an alpine desert ecosystem, Northeast Qinghai-Tibet Plateau, China.

    Science.gov (United States)

    Wu, Huawu; Li, Xiao-Yan; Jiang, Zhiyun; Chen, Huiying; Zhang, Cicheng; Xiao, Xiong

    2016-01-15

    Plant water use patterns reflect the complex interactions between different functional types and environmental conditions in water-limited ecosystems. However, the mechanisms underlying the water use patterns of plants in the alpine desert of the Qinghai-Tibet Plateau remain poorly understood. This study investigated seasonal variations in the water sources of herbs (Carex moorcroftii, Astragalus adsurgens) and shrubs (Artemisia oxycephala, Hippophae rhamnoides) using stable oxygen-18 isotope methods. The results indicated that the native herbs (C. moorcroftii, A. adsurgens) and one of the shrubs (A. oxycephala) mainly relied on water from the shallow layer (0-30 cm) throughout the growing season, while the introduced shrub (H. rhamnoides) showed plasticity in switching between water from shallow and deep soil layers depending on soil water availability. All studied plants primarily depended on water from shallow soil layers early in the season. The differences of water use patterns between the introduced and native plants are closely linked with the range of active root zones when competing for water. Our findings will facilitate the mechanistic understanding of plant-soil-water relations in alpine desert ecosystems and provide information for screening introduced species for sand fixation.

  18. Response pattern of amino compounds in phloem and xylem of trees to soil drought depends on drought intensity and root symbiosis.

    Science.gov (United States)

    Liu, X-P; Gong, C-M; Fan, Y-Y; Eiblmeier, M; Zhao, Z; Han, G; Rennenberg, H

    2013-01-01

    This study aimed to identify drought-mediated differences in amino nitrogen (N) composition and content of xylem and phloem in trees having different symbiotic N(2)-fixing bacteria. Under controlled water availability, 1-year-old seedlings of Robinia pseudoacacia (nodules with Rhizobium), Hippophae rhamnoides (symbiosis with Frankia) and Buddleja alternifolia (no such root symbiosis) were exposed to control, medium drought and severe drought, corresponding soil water content of 70-75%, 45-50% and 30-35% of field capacity, respectively. Composition and content of amino compounds in xylem sap and phloem exudates were analysed as a measure of N nutrition. Drought strongly reduced biomass accumulation in all species, but amino N content in xylem and phloem remained unaffected only in R. pseudoacacia. In H. rhamnoides and B. alternifolia, amino N in phloem remained constant, but increased in xylem of both species in response to drought. There were differences in composition of amino compounds in xylem and phloem of the three species in response to drought. Proline concentrations in long-distance transport pathways of all three species were very low, below the limit of detection in phloem of H. rhamnoides and in phloem and xylem of B. alternifolia. Apparently, drought-mediated changes in N composition were much more connected with species-specific changes in C:N ratios. Irrespective of soil water content, the two species with root symbioses did not show similar features for the different types of symbiosis, neither in N composition nor in N content. There was no immediate correlation between symbiotic N fixation and drought-mediated changes in amino N in the transport pathways.

  19. Characteristics of leaf areas of plantations in semiarid hills and gully loess regions

    Institute of Scientific and Technical Information of China (English)

    Jing YIN; Fan HE; Guoyu QIU; Kangning HE; Jinghui TIAN; Weiqiang ZHANG; Yujiu XIONG; Shaohua ZHAO; Jianxin LIU

    2009-01-01

    The objectives of our study were to explore the relationship of leaf area and stand density and to find a convenient way to measure stand leaf areas. During the 2004 growing season, from May to October, we used direct and indirect methods to measure the seasonal variation of the leaf areas of tree and shrub species. The trees were from Robinia pseudoacacia stands of four densities (3333 plants/hm2, 1666 plants/hm2, 1111 plants/hm2, and 833 plants/hm2) and Platycladus orientalis stands of three densities (3333 plants/hm2, 1666 plants/hm2, and 1111 plants/hm2). The shrub species were Caragana korshinskii, Hippophae rhamnoides, and Amorpha fruticosa. Based on our survey data, empirical formulas for calculating leaf area were obtained by correlating leaf fresh weight, diameter of base branches, and leaf areas. Our results show the following: 1) in September, the leaf area and leaf area index (LAI) of trees (R. pseudoacacia and P orientalis) reached their maximum values, with LAI peak values of 10.5 and 3.2, respectively. In August, the leaf area and LAI of shrubs (C. korshinskii, H. rhamnoides, and A. fruticosa) reached their maximum values, with LAI peak values of 1.195, 1.123, and 1.882, respectively. 2) There is a statistically significant power relation between leaf area and leaf fresh weight for R. pseudoacacia. There are significant linear relationships between leaf area and leaf fresh weight for P. orientalis, C. korshinskii, H. rhamnoides, and A. fruticosa. Moreover, there is also a significant power relation between leaf area and diameter of base branches for C. korshinskii. There are significant linear relations between leaf area and diameter of base branches of H. rhamnoides and A. fruticosa. 3) In the hills and gully regions of the Loess Plateau, the LAIs of R. pseudoacacia stand at different densities converged after the planted stands entered their fast growth stage. Their LAI do not seem to be affected by its initial and current density. The same is true

  20. Consumer-led development of novel sea-buckthorn based beverages

    DEFF Research Database (Denmark)

    Geertsen, Julie Leth; Allesen-Holm, Bodil Helene; Giacalone, Davide

    2016-01-01

    characteristics were the most important drivers of consumer preferences within this product category. Practical Applications This study offers new insights into consumers’ perception of novel food products of local origin. A key issue with such ingredients is that they have unique sensory properties which...

  1. Variations in seed germination of Hippophae salicifolia with different presoaking treatments

    Institute of Scientific and Technical Information of China (English)

    S. Airi; I.D. Bhatt; A. Bhatt; R.S. Rawal; U. Dhar

    2009-01-01

    Mature seeds of H. salicifolia, collected from five provenances (i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti) in west Himalaya, India, were treated with stratification (at 4oC for 15, 30 and 60 days) and in different concentrations of GA3 (5, 10, 20 mM), KNO3 (50, 100, 200 mM) and Thiourea (50, 100, 200 mM) solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control (24%-30%). The seeds treated with Thiourea (100 mM) have highest germination rate (76%-83% for different seed sources), followed by those (63%-71% for different seed sources) pretreated with stratification (4oC, 30 days). GA3 treatment significantly shortens the mean germination time (MGT) and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea (100 mM) and stratification (at 4oC) treatments for seed germination are recommended for mass multiplication through seeds of H. salicifolia in village/forest nurseries of the west Himalaya, India.

  2. Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Chao; LIU Guo-bin; XUE Sha; and XIAO Lie

    2013-01-01

    The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows:two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a signiifcant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions signiifcantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

  3. Relationships between xylem embolism and eco-physiological indices in eight woody plants in sltu(Ⅱ):The relationship with photosynthetic eco-physiological indices

    Institute of Scientific and Technical Information of China (English)

    AN Feng; CAI Jing; JIANG Zaimin; ZHANG Yuanying; ZHAO Pingjuan; ZHANG Shuoxin

    2007-01-01

    The relationship between xylem embolism and eco-physiology indices (I.e.photosynthetic available radiation,temperature,relative humidity,photosynthetic rate,transpiration rate,stomatal conductance and water use efficiency) in eight tree species was investigated in situ.The species studied,Robinia pseudoacacia L.,Acer truncatum Bge.,Hippophae rhamnoides L.,Ulmus pumila L.,Pinus tabulaeformis Carr., Pinus bungeana Zucc.ex Endl.,Ligustrum lucidum Ait.,and Salix matsudana Koidz.f.pendula Schneid,grow well on the Xilin campus of Northwest A&F University.Results indicated that photosynthetic available radiation,air temperature and relative humidity can affect xylem embolism by daily adjustment of stomatal conductance,transpiration rate and water relations of a tree.Embolism was a common case in the daily growth of the plants,and there was some correlation between xylem embolism and photosynthetic rate,transpiration rate,stornatal conductance,and water use efficiency.Embolism may thus be an adaptive mechanism by some tree species to water stress.

  4. [Community diversity of bacteria and arbuscular mycorrhizal fungi in the rhizosphere of eight plants in Liudaogou watershed on the Loess Plateau China].

    Science.gov (United States)

    Feng, Ye; Tang, Ming; Chen, Hui; Cong, Wei

    2012-01-01

    Terminal restriction fragment length polymorphism (T-RFLP) was used to examine the community diversity of bacteria and arbuscular mycorrhizal fungi (AMF) and their interrelation in the rhizosphere of 8 plants in the Liudaogou watershed in Shenmu County. The objective was to obtain diversity indices and provide theoretical basis for ecological restoration. Results showed significant variations in the species and abundances of rhizospheric bacteria and AMF associated with 8 plants. Among these, the Shannon diversity index of rhizospheric bacteria was the highest for Robinia pseudoacacia (4.01) and the lowest for Salix babylonica (2.18), whereas the Shannon diversity index of rhizospheric AMF was the highest for Populus simonii (2.07) and the lowest for Hippophae rhamnoides (1.21). Cluster analysis and redundancy analysis indicated a significant difference in associated microbial community structure, while the similarity among community diversity of rhizospheric bacteria and AMF associated with specific plants was also found. There was a significant correlation between diversity indices of bacteria and AMF (P < 0.01). Associated microbial community diversity was influenced primarily by organic matter and total nitrogen content. Our work demonstrated strong impacts of plant species and rhizospheric environment on associated microbial community structure. Due to the high diversity indices of rhizospheric bacteria and AMF, R. pseudoacacia was considered to be a pioneer plant species for vegetation restoration in the Liudaogou watershed.

  5. An approach for detecting five typical vegetation types on the Chinese Loess Plateau using Landsat TM data.

    Science.gov (United States)

    Wang, Zhi-Jie; Jiao, Ju-Ying; Lei, Bo; Su, Yuan

    2015-09-01

    Remote sensing can provide large-scale spatial data for the detection of vegetation types. In this study, two shortwave infrared spectral bands (TM5 and TM7) and one visible spectral band (TM3) of Landsat 5 TM data were used to detect five typical vegetation types (communities dominated by Bothriochloa ischaemum, Artemisia gmelinii, Hippophae rhamnoides, Robinia pseudoacacia, and Quercus liaotungensis) using 270 field survey data in the Yanhe watershed on the Loess Plateau. The relationships between 200 field data points and their corresponding radiance reflectance were analyzed, and the equation termed the vegetation type index (VTI) was generated. The VTI values of five vegetation types were calculated, and the accuracy was tested using the remaining 70 field data points. The applicability of VTI was also tested by the distribution of vegetation type of two small watersheds in the Yanhe watershed and field sample data collected from other regions (Ziwuling Region, Huangling County, and Luochuan County) on the Loess Plateau. The results showed that the VTI can effectively detect the five vegetation types with an average accuracy exceeding 80 % and a representativeness above 85 %. As a new approach for monitoring vegetation types using remote sensing at a larger regional scale, VTI can play an important role in the assessment of vegetation restoration and in the investigation of the spatial distribution and community diversity of vegetation on the Loess Plateau.

  6. Role of the different planting age of seabuckthorn forests to soil amelioration in coal mining subsidence land

    Institute of Scientific and Technical Information of China (English)

    Yinli Bi; Yanxu Zhang

    2014-01-01

    To investigate the effects of seabuckthorn (Hippophae rhamnoides) on soil amelioration, using the space replacement method, soil physical and chemical indexes as well as the microorganism quantity and soil enzyme activities were analyzed. The results showed that:the soil bulk density of surface soil decreased and soil porosity and field capacity increased after afforestation with seabuckthorn. The plant was found to effectively reduce the soil pH, increase the soil conductivity, soil organic matters and available nutrients. Soil microorganism quantity, soil enzyme activities were both higher in 0–20 cm layer than in 20–40 cm layer. With the increase years of remediation with seabuckthorn, the quantity of soil microorganism and enzyme activities were increasing to a higher level 5 to 8 years later. Our study indicates that seabuckthorn can effectively improve soil physical and chemical properties, increase the quantity of soil microor-ganisms and enzyme activities, which is of great significance for the ecosystem restoration in mining areas.

  7. Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction.

    Directory of Open Access Journals (Sweden)

    Yun Luo

    Full Text Available Isorhamnetin (Iso is a flavonoid compound extracted from the Chinese herb Hippophae rhamnoides L. Previous studies have revealed its anti-cancer, anti-inflammatory, and anti-oxidant activities. This study investigated the ability of Iso to inhibit oxidized low-density lipoprotein (ox-LDL-induced cell apoptosis in THP-1-derived macrophages. The effects of Iso on atherosclerosis in vivo were also evaluated in apolipoprotein E knockout (ApoE-/- mice fed a high fat diet.Iso showed significant inhibitory effects on ox-LDL-induced THP-1-derived macrophage injuries via decreasing reactive oxygen species levels, lipid deposition, and caspase-3 activation, restoring mitochondrial membrane potential, reducing the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL-positive cells, and regulating apoptosis-related proteins. We also determined the protective effects of Iso by PI3K/AKT activation and HO-1 induction. Iso reduced the atherosclerotic plaque size in vivo in ApoE-/- mice as assessed by oil red O, Sudan IV staining, and CD68-positive cells, and reduced macrophage apoptosis as assessed by caspase-3 and TUNEL assays in lesions.In conclusion, our results show that Iso inhibited atherosclerotic plaque development in ApoE-/- mice by PI3K/AKT activation and HO-1 induction.

  8. Isorhamnetin protects against cardiac hypertrophy through blocking PI3K-AKT pathway.

    Science.gov (United States)

    Gao, Lu; Yao, Rui; Liu, Yuzhou; Wang, Zheng; Huang, Zhen; Du, Binbin; Zhang, Dianhong; Wu, Leiming; Xiao, Lili; Zhang, Yanzhou

    2017-02-07

    Isorhamnetin, a flavonoid compound extracted from the Chinese herb Hippophae rhamnoides L., is well known for its anti-inflammatory, anti-oxidative, anti-adipogenic, anti-proliferative, and anti-tumor activities. However, the role of isorhamnetin in cardiac hypertrophy has not been reported. The aims of the present study were to find whether isorhamnetin could alleviate cardiac hypertrophy and to define the underlying molecular mechanisms. Here, we investigated the effects of isorhamnetin (100 mg/kg/day) on cardiac hypertrophy induced by aortic banding in mice. Cardiac hypertrophy was evaluated by echocardiographic, hemodynamic, pathological, and molecular analyses. Our data demonstrated that isorhamnetin could inhibit cardiac hypertrophy and fibrosis 8 weeks after aortic banding. The results further revealed that the effect of isorhamnetin on cardiac hypertrophy was mediated by blocking the activation of phosphatidylinositol 3-kinase-AKT signaling pathway. In vitro studies performed in neonatal rat cardiomyocytes confirmed that isorhamnetin could attenuate cardiomyocyte hypertrophy induced by angiotensin II, which was associated with phosphatidylinositol 3-kinase-AKT signaling pathway. In conclusion, these data indicate for the first time that isorhamnetin has protective potential for targeting cardiac hypertrophy by blocking the phosphatidylinositol 3-kinase-AKT signaling pathway. Thus, our study suggests that isorhamnetin may represent a potential therapeutic strategy for the treatment of cardiac hypertrophy and heart failure.

  9. Isorhamnetin suppresses colon cancer cell growth through the PI3K‑Akt‑mTOR pathway.

    Science.gov (United States)

    Li, Chuan; Yang, Xi; Chen, Cheng; Cai, Shaoxin; Hu, Junbo

    2014-03-01

    Isorhamnetin, a flavonoid isolated from the fruits of herbal medicinal plants, such as Hippophae rhamnoides L., exerts anticancer effects similar to other flavonoids. However, the effect of isorhamnetin on colorectal cancer (CRC) and the underlying molecular mechanism are unclear. This study aimed to determine the effect of isorhamnetin on the proliferation of cells from the human CRC cell lines, HT‑29, HCT116 and SW480. It was demonstrated that isorhamnetin suppressed the proliferation of cells from all three cell lines, induced cell cycle arrest at the G2/M phase and suppressed cell proliferation by inhibiting the PI3K‑Akt‑mTOR pathway. Isorhamnetin also reduced the phosphorylation levels of Akt (ser473), phosph‑p70S6 kinase and phosph‑4E‑BP1 (t37/46) protein, and enhanced the expression of Cyclin B1 protein. Therefore, this compound was revealed to be a selective PI3K‑Akt‑mTOR pathway inhibitor, and may be a potent anticancer agent for the treatment of CRC, as it restrains the proliferation of CRC cells.

  10. Genetic structure in the seabuckthorn carpenter moth (Holcocerus hippophaecolus in China: the role of outbreak events, geographical and host factors.

    Directory of Open Access Journals (Sweden)

    Jing Tao

    Full Text Available Understanding factors responsible for structuring genetic diversity is of fundamental importance in evolutionary biology. The seabuckthorn carpenter moth (Holcocerus hippophaecolus Hua is a native species throughout the north of China and is considered the main threat to seabuckthorn, Hippophae rhamnoides L. We assessed the influence of outbreaks, environmental factors and host species in shaping the genetic variation and structure of H. hippophaecolus by using Amplified Fragment Length Polymorphism (AFLP markers. We rejected the hypothesis that outbreak-associated genetic divergence exist, as evidenced by genetic clusters containing a combination of populations from historical outbreak areas, as well as non-outbreak areas. Although a small number of markers (4 of 933 loci were identified as candidates under selection in response to population densities. H. hippophaecolus also did not follow an isolation-by-distance pattern. We rejected the hypothesis that outbreak and drought events were driving the genetic structure of H. hippophaecolus. Rather, the genetic structure appears to be influenced by various confounding bio-geographical factors. There were detectable genetic differences between H. hippophaecolus occupying different host trees from within the same geographic location. Host-associated genetic divergence should be confirmed by further investigation.

  11. Impacts of mixed litter decomposition from Robinia pseudoacacia and other tree species on C loss and nutrient release in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    Xiaoxi Zhang; Zengwen Liu; Bochao Zhu; Yuanhao Bing; Nhu Trung Luc; Liangzhen Du; Zhenhua Zhu

    2016-01-01

    The productivity of Robinia pseudoacacia (R.p.) pure forest usually declines at the late growth stage, and reforming it into mixed forests could be a promising way to resolve this problem. When choosing a suitable tree species that can be mixed with R.p., the interspecific relationship is an important issue. Therefore, we gathered the autumn litter fall from R.p. and 10 other species from the Loess Plateau of China were mixed in dual species litterbags (R.p. ? each other species) and buried them in soil for a 345 days lab decay incubation. We measured the litter mass loss and nutrient contents to determine whether the nutrient release was affected by mixed species litter decomposition. The impacts of mixed litter decomposition on macro-elements release were more obvious than on micro-elements. The litters with similar substrate quality might show variable impacts on nutrients release in mixed decomposition. The C loss and release of nutrient was improved by descending order when R.p. litter was mixed with Hippophae rhamnoides, Ulmus pumila, Populus simonii, Larix principis-rupprechtii and Quercus liaotun-gensis (Q.l.). But, except for Q.l., only the other species were recommended as suitable mix-plants for R.p. since promoting a high turnover of the nutrient in the litter compartment and a rapid availability for tree.

  12. HOST PLANTS AND CLIMATIC PREFERENCES OF THE INVASIVE SPECIES METCALFA PRUINOSA (SAY 1830 (HEMIPTERA: FLATIDAE IN SOME PLACES FROM SOUTHERN ROMANIA

    Directory of Open Access Journals (Sweden)

    Daniela Barbuceanu

    2015-12-01

    Full Text Available Observations carried out in May-September 2015 in two sites of Southern Romania reveal a rich spectrum of host plants for Metcalfa pruinosa, which consists of 204 species in 56 families. The species it is noticed on weeds and cultivated plants. The remarkable polyphagia of this species, the lack of natural enemies, and the climatic conditions of 2015 - warm and dry summer, had lead to an invasion of M. pruinosa, in the researched areas; the highest numerical abundances are noticed in shady habitats. Furthermore, on herbs, such as Levisticum officinale, Artemisia dracunculus, Ocimum basilicum, Mentha spp., usually avoided by pests, were observed colonies of the species. It is recorded high numerical abundance on fruit trees and shrubs: Hippophaë rhamnoides, Juglans regia, Prunus cerasus, Vitis vinifera, Rubus idaeus. The harmful effect occurs on apple trees Romus 1 variety as a result of the association with another pest of American origin, Eriosoma lanigerum, situation that favors the attack of the Erwinia amylovora bacteria, causing the collapse of the tree. It is found that altitudes higher than 200 m do not represent a limitative factor in the spreading of species, one of the investigated sites being located at 304 m altitude.

  13. Micromonospora is a normal occupant of actinorhizal nodules

    Indian Academy of Sciences (India)

    Lorena Carro; Petar Pujic; Martha E Trujillo; Phillipe Normand

    2013-11-01

    Actinorhizal plants have been found in eight genera belonging to three orders (Fagales, Rosales and Cucurbitales). These all bear root nodules inhabited by bacteria identified as the nitrogen-fixing actinobacterium Frankia. These nodules all have a peripheral cortex with enlarged cells filled with Frankia hyphae and vesicles. Isolation in pure culture has been notoriously difficult, due in a large part to the growth of fast-growing contaminants where, it was later found, Frankia was slow-growing. Many of these contaminants, which were later found to be Micromonospora, were obtained from Casuarina and Coriaria. Our study was aimed at determining if Micromonospora were also present in other actinorhizal plants. Nodules from Alnus glutinosa, Alnus viridis, Coriaria myrtifolia, Elaeagnus x ebbingei, Hippophae rhamnoides, Myrica gale and Morella pensylvanica were tested and were all found to contain Micromonospora isolates. These were found to belong to mainly three species: Micromonospora lupini, Micromonospora coriariae and Micromonospora saelicesensis. Micromonospora isolates were found to inhibit some Frankia strains and to be innocuous to other strains.

  14. Evidence that some Frankia sp. strains are able to cross boundaries between Alnus and Elaeagnus host specificity groups.

    Science.gov (United States)

    Bosco, M; Fernandez, M P; Simonet, P; Materassi, R; Normand, P

    1992-05-01

    Phenotypic and genotypic methods were used to prove the existence of Frankia strains isolated from an Elaeagnus sp. that are able to cross the inoculation barriers and infect Alnus spp. also. Repeated cycles of inoculation, nodulation, and reisolation were performed under axenic conditions. Frankia wild-type strain UFI 13270257 and three of its coisolates did exhibit complete infectivity and effectiveness on Elaeagnus spp. and Hippophaë rhamnoides and variable infectivity on Alnus spp. Microscopical observation of host plant roots showed that these strains are able to infect Alnus spp. by penetrating deformed root hairs. Reisolates obtained from nodules induced on monoxenic Alnus glutinosa, Alnus incana, and Elaeagnus angustifolia resembled the parent strains in host infectivity range, in planta and in vitro morphophysiology, isoenzymes, and nif and rrn restriction fragment length polymorphisms, thus fulfilling Koch's postulates on both host plant genera. Alnus and Elaeagnus group-specific polymerase chain reaction DNA amplifications, DNA-DNA hybridizations, and partial gene sequences coding for 16S rRNA provided evidence for the genetic uniformity of wild-type strains and their inclusion into one and the same genomic species, clearly belonging to the Elaeagnus group of Frankia species.

  15. Possibilities of cultivating ornamental trees and shrubs under conditions of air pollution with oxides of sulfur

    Energy Technology Data Exchange (ETDEWEB)

    Bialobok, S.; Bartkowiak, S.; Rachwal, L.

    1974-01-01

    The field work conducted has shown that high concentrations of SO/sub 2/ in the air can be withstood by the following trees and shrubs. Trees: Acer campestris, A. platanoides, Ailanthus altissima, Aesculus hippocastanum, Morus alba, Platanus acerifolia, Pinus strobur, P. nigra, Populus Berolinensis, P. candicans, P. Hybr. 27, P. Marilandica, P. simonii, P. Serotina, Quercus robus, Robinia pseudoacacia. Shrubs: Caragana arborescens, Crataegus oxyacantha, C. monogyna, Cerasus mehaleb, Forsythia/most of the species and varieties/, Ligustrum vulgare, Philadelphus coronaria, Ptelea trifoliata, Sambucus nigra, Salix caprea, Sorbaria sorbifolia, Sorbus aucuparia, Taxus baccata. For the selection of trees and shrubs in the laboratory, high concentrations of SO/sub 2/ were used (60-150 ppm for a period of 10 minutes). Experiments were conducted on cut shrubs kept in the gas chambers. In order to estimate the degree of their injury, they were transferred to a shaded greenhouse. A concentration of 65 ppm of SO/sub 2/ could be withstood by the following Forsythias: Forsythia intermedia Primulina, F. Densiflora, F. Spectabilis, F. giraldina, F. suspensa, F. koreana, F. ovata, F. japonica and Hippophae rhamnoides. A concentration of 130 ppm could be withstood only by F. intermedia Vitelina. A similarly high concentration of SO/sub 2/ could be withstood by shoots and leaves of Ailanthus girladii Duclouxii and by Platanus acerifolia. From among the lilacs Syringa pekinensis and S. amurensis proved resistant to high concentrations of SO/sub 2/.

  16. Soil and fertilizer amendments and edge effects on the floral succession of pulverized fuel ash

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, P. [Roehampton University, London (United Kingdom). Whitelands College

    2009-01-15

    Plots of fresh pulverized fuel ash (PFA, an industrial waste) were inoculated with soils from existing PFA sites and fertilizers in a factorial design, then left unmanaged for 12 years during which time the floral development and soil chemistry were monitored annually. For the first 3 years, the site supported a sparse mix of chenopods (including the scarce Chenopodium glaucum) and halophytes. As salinity declined, ruderals, legumes, and grasses plus the fire-site moss Funaria hygrometrica colonized, followed by Festuca arundinacea grassland (NVC community MG12) and Hippophae rhamnoides scrub. Dactylorhiza incarnata (orchidacea) appeared after 7 years, but only in plots that had received soil from existing orchid colonies. Four years later, a larger second generation of Dactylorhiza appeared, but only in the central zone of the site where vegetation was thinnest. By year 12, the site was dominated by coarse grasses and scrub, with early successional species persisting only in the sparsely vegetated center, where nitrate levels were lowest. This edge effect is interpreted as centripetal encroachment, a process of potentially wider concern for the conservation of low-fertility habitat patches. Overall, seed bank inoculation seems to have introduced few but desirable species (D. incarnata, Pyrola rotundifolia, some halophytes, and annuals), whereas initial application of organic fertilizer had long-lasting ({ge} 10 years) effects on cover and soil composition.

  17. 沙棘果实成熟期一些生化成分的变化%The Changes of Some Biochemical Constituents along Seabuckthorn Fruit Ripening

    Institute of Scientific and Technical Information of China (English)

    M.antonelli; A.Raffo; N.Nardo; F.Paoletti; 何京亮; 王涛

    2009-01-01

    为了分析沙棘浆果成分的组成特点(Hippophae rhamnoides L.),研究人员进行了若干关于品种、原产地、成熟时间的研究(Gao等,2000;Kallio等1999;Tang和Tigerstedt,2001)。这些研究大部分集中在微量化合物组成成分的测定方面。已知该微量化合物因其抗氧化性有保护健康的作用(引自Gao等)。在这个意义上,研究人员发现沙棘浆果富含抗坏血酸、酚类化合物、生育酚类、类胡萝卜素等。尽管值得深入研究,但是据文献,一些如黄酮醇和类胡萝卜素的复合物类还没被广泛研究。(Hakkinen等,1999;Yang和Kallio,2002)。

  18. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Asad, Muhammad Hassham Hassan Bin; Akram, Muhammad Rouf; Kalsoom Khan, Abida; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed. PMID:26448818

  19. Isolation and analysis of vitamin B12 from plant samples.

    Science.gov (United States)

    Nakos, M; Pepelanova, I; Beutel, S; Krings, U; Berger, R G; Scheper, T

    2017-02-01

    Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n=6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37μg/100g dry weight), in Elymus (26μg/100g dry weight) and in Inula helenium (11μg/100g dry weight).

  20. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Directory of Open Access Journals (Sweden)

    Saima Jadoon

    2015-01-01

    Full Text Available The exposure to ultraviolet radiations (UVR is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS, leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  1. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity.

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Bin Asad, Muhammad Hassham Hassan; Akram, Muhammad Rouf; Khan, Abida Kalsoom; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  2. 沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响%Effects of sea buckthorn pulp oil and sea buckthorn seed oil on radiation skin lesions in rats

    Institute of Scientific and Technical Information of China (English)

    王养正; 吴英; Heikki Kallio; Yang Baoru; 王军宪; 王秉文; 崔刚

    2008-01-01

    目的 观察沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响.方法 实验用深部X线治疗机,每次以3000cGy的剂量,照射两次,造成大白鼠臀部放射性皮肤损伤.待动物症状明显后,将Ⅱ度皮肤损伤的动物随机分为6组,即阴性对照组、沙棘果油高剂量组、沙棘果油低剂量组、沙棘籽油高剂量组、沙棘籽油低剂量组以及阳性对照组.沙棘果油及籽油高剂量组,每天在损伤部位涂药两次,而其低剂量组每天涂药一次,阴性对照组每天涂灭菌的注射用水一次,阳性对照组每天涂Solcoseryl-Jelly软膏一次.结果 肉眼观察结果显示,连续用药10d后,沙棘果油、籽油及Solcoseryl-Jelly组动物的局部红斑、水肿明显减轻,创面分泌物明显减少,用药2周后,上述症状明显消退,与阴性对照组比较,明显好转;另外,沙棘果油、籽油及Solcoseryl-Jelly组的创面愈合时间明显缩短.光学显微镜观察结果显示,沙棘果油、籽油及Solcoseryl-Jelly组的皮肤损伤明显减轻,炎症反应亦明显减轻,创面修复明显.电子显微镜观察结果显示,阴性对照组的超微结构明显受损,而沙棘果油、籽油及Solcoseryl-Jelly组的损伤明显减轻.结论 沙棘果油及沙棘籽油对大鼠放射性皮肤损伤有明显的治疗作用.

  3. 77 FR 4810 - Pesticide Products; Receipt of Applications To Register New Uses

    Science.gov (United States)

    2012-01-31

    ..., native currant, red currant, sea buckthorn, wild raspberry, amur river grape, hardy kiwifruit, maypop..., jostaberry, native currant, red currant, sea buckthorn, wild raspberry, amur river grape, hardy kiwifruit..., sunberry, Amur river grape, gooseberry, kiwifruit, hardy, maypop, muscadines, schisandra berry,...

  4. Root Ecological Niche Index and Root Distribution Characteristics of Artificial Phytocommunities in Rehabilitated Fields

    Institute of Scientific and Technical Information of China (English)

    Hu Jianzhong; Zhen Jiali; Shen Jingyu

    2006-01-01

    In the implementation phase of the Conversion of Cropland to Forest and Grassland (CCFG) project in China,it is important,from a scientific point of view,to recognize phytocommunities' characteristics,species compatibility,and ecological function.The ecological niche that roots occupy,their abundance and distribution,and the factors that affect them must be acknowledged.Following the methodology of community ecology,the total root mass of a phytocommunity is measured as cubic volume.Root biomass,length,and the number of roots in every diameter class,for each soil layer and for each plant species,are regarded as observation variables.In the first instance therefore,a new method to calculate the root ecological niche index (REND is proposed,embracing the entire phytocommunity of plantations.Using the new method,the roots of pbytocommunities in Datong County,Qinghai Province (one of the counties selected for the national CCFG experiment),are dealt with in this paper.The results show that most of the vertical distributions of plant roots belong to the type wherein the roots are concentrated in the topsoil layer (0-20 cm),far more than those in the lower soil layers.The RENI of pbytocommunities is higher than that of pure stands or monocultures.The distribution of RENI by root diameter can be divided into four types:J-type,inverse J-type,recumbent S-type,and U-type.RENI is positively correlated with the wet biomass of aboveground level stems,branches,and plant leaves,and with the species richness of phytocommunities.Although the RENIs of plantations in rehabilitated fields are a little lower than those of natural forests,they are higher than those of cultivated crops.The RENIs of three community types (Picea crassifolia+Hippophae rhamnoides ssp.sinensis,H.rhamnoides ssp.sinensis,and P.crassifolia) in rehabilitated fields benefit greatly from the restoration project.The implementation of the CCFG project is important for the increase in RENI and the multiple functions of

  5. 大兴沙地中11个树种基于根系形态的适应性%Adaptability of Eleven Tree Species Based on Root Characteristics in Daxing Sandy Land, Beijing

    Institute of Scientific and Technical Information of China (English)

    柴源; 徐程扬; 袁士保; 陈华; 段树生

    2012-01-01

    An experiment was conducted to study the adaptability of 11 tree species in Daxing sandy land in Beijing according to aboveground growth, root vertical distribution, and root morphological variation by means of systematic clustering. Results showed that tree height, diameter at breast height, shoot length, shoot relative growth, root length, root surface area, root volume and specific root length were obviously different among the eleven tree species (p<0.001). Strong differentiation of fine roots was observed in Ailanthus altissima, Catalpa bungei, and relative strong differentiation of fine roots in Gleditsia sinensis, Sabina chinensis and Koelreuteria paniculate, indicating that these tree species were drought resistant by using more soil resources. The drought avoidance of Ziziphus jujuba, Elaeagnus angustifolia and Hippophae rhamnoides was observed by decreasing fine roots less than 2 mm in diameter, increasing medium roots 2? mm or more than 5 mm in diameter, and deepening root distribution. Pinus sylvestris var. mongolica was drought tolerant by decreasing the growth of aboveground parts and root system; while Moms alba trees exhibited poor adaptability to sandy land.%通过地上生长量、根系垂直分布和形态特征分析以及系统聚类分析,对11个树种在北京大兴沙地生长的适宜性进行了研究.结果表明:各树种的树高、直径、新梢生长量和相对生长以及根系长度、根系表面积、根系体积和比根长等指标均有极显著的差异(p<0.001).其中,臭椿(Ailanthus altissima)、楸树(Catalpa bungei)细根分化极强,皂角(Gleditsia sinensis)、圆柏(Sabina chinensis和栾树(Koelreuteria paniculata)根系分化能力较强,通过较高的土壤资源消耗体现抗旱能力;酸枣(Ziziphus jujuba)、沙枣(Elaeagnus angustifolia)和沙棘(Hippophae rhamnoides)通过降低直径小于2 mm根系分化、提高直径2~5mm和大于5 mm根系生长和分布深度体现

  6. Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS

    Science.gov (United States)

    Suchal, Kapil; Bhatia, Jagriti; Malik, Salma; Malhotra, Rajiv Kumar; Gamad, Nanda; Goyal, Sameer; Nag, Tapas C.; Arya, Dharamvir S.; Ojha, Shreesh

    2016-01-01

    Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt–eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression. PMID:27445803

  7. HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas

    Directory of Open Access Journals (Sweden)

    Raluca Maria Pop

    2013-11-01

    Full Text Available . High performance liquid chromatography (HPLC with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: Taraxacum officinalis (1, Cynara scolimus (2, Silybum marianum (3, Hypericum perforatum (4,  Chelidonium majus (5, Lycopodium clavatum (6 and  Hippophae rhamnoides (7  leaves and fruits.  Two products (A and B were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol were analyzed using HPLC-UV for their phenolics’ fingerprint and composition. The qualitative (untargeted analysis and quantitative (targeted analysis results were further compared using Principal Component Analysis (PCA in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were trans-cinnamic, chlorogenic, caffeic and p-coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids. In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.

  8. Land-Use Conversion Changes the Multifractal Features of Particle-Size Distribution on the Loess Plateau of China.

    Science.gov (United States)

    Sun, Caili; Liu, Guobin; Xue, Sha

    2016-01-01

    Analyzing the dynamics of soil particle-size distributions (PSDs), soil nutrients, and erodibility are very important for understanding the changes of soil structure and quality after long-term land-use conversion. We applied multifractal Rényi spectra (Dq) and singularity spectra (f(α)) to characterize PSDs 35 years after conversions from cropland to shrubland with Caragana microphylla (shrubland I), shrubland with Hippophae rhamnoides (shrubland II), forested land, and grassland on the Loess Plateau of China. Multifractal parameters (capacity dimension (D₀), entropy dimension (D₁), D₁/D₀, correlation dimension (D₂), and Hölder exponent of order zero (α₀)) were used to analyze the changes of PSDs. Dq and f(α) characterized the PSDs well and sensitively represented the changes in PSDs after conversion. All types of land-use conversion significantly improved the properties of the topsoil (0-10 cm), but the effect of shrubland I and even forested land decreased with depth. All types of land-use conversion significantly increased D₁ and D₂ in the topsoil, and D₁ and D₂ in the 10-50 cm layers of shrubland II, forested land, and grassland and D₁ in the 50-100 cm layers of shrubland II were significantly higher relative to the control. Both D₁ and D₂ were positively correlated with the contents of soil nutrients and fine particles and were negatively correlated with soil erosion, indicating that D₁ and D₂ were potential indices for quantifying changes in soil properties and erosion. In conclusion, all types of land-use conversion significantly improved the conditions of the topsoil, but conversion from cropland to shrubland II, forested land, and grassland, especially shrubland II and grassland, were more effective for improving soil conditions in deeper layers.

  9. Soil characterization and differential patterns of heavy metal accumulation in woody plants grown in coal gangue wastelands in Shaanxi, China.

    Science.gov (United States)

    Yakun, Shi; Xingmin, Mu; Kairong, Li; Hongbo, Shao

    2016-07-01

    Soil contamination by heavy metals in coal mine wastelands is a significant environmental issue in most developing countries. The purpose of this study is to evaluate contamination characteristics in the coal mine wastelands of Sanlidong coal mine, Tongchuan, China. To achieve this goal, we conducted field sampling work, followed by further analysis of the properties of soil contamination and accumulation characteristics in woody plants. At this site, the pH value ranged from 4.41 to 7.88, and the nutrient content of the soil rose gradually with the time after deposition due to the weathering effect improving the soil quality. Meanwhile, the levels of Cd, Cr, Cu, Ni, and Zn gradually decreased with the passage time. Generally, heavy metal contamination was found to be more serious in the discharge refuse area, with Cd contamination at moderate or heavy levels; Ni, Zn, and Cu contamination at light levels; and with no Cr contamination. The geoaccumulation index (I geo) was highest for Cd (2.38-3.14), followed by Ni, Zn, Cu, and Cr. Heavy metals accumulated on the lower slopes and spread to the surrounding areas via hydrodynamic effects and wind. According to transfer and enrichment coefficient analyses, Robinia pseudoacacia, Ulmus pumila, and Hippophae rhamnoides with considerable biomass could be used as pollution-resistant tree species for vegetation restoration. This study provided a theoretical basis for the restoration of the ecological environment in the mining area. This report described a link between heavy metal contamination of soils and growth dynamics of woody plants in China.

  10. Intra-herb pharmacokinetics interaction between quercetin and isorhamentin

    Institute of Scientific and Technical Information of China (English)

    Ke LAN; Jian-lin HE; Yang TIAN; Fei TAN; Xue-hua JIANG; Ling WANG; Li-ming YE

    2008-01-01

    Aim: Quercetin and isorhamnetin are common constituents of some herb extracts, such as extracts of gingko leaves and total flavones of Hippophae rhamnoides L. The intra-herb pharmacokinetics interactions between isorhamnetin and quercetin were investigated in the present study. Methods: Human MDR1 cDNA transfected MDCKII cells were used to validate whether isorhamnein interacted with P-gp. Caco-2 transport assays and a randomized, 3-way crossover pharmacokinetics study in rats were used to investigate the pharmacokinetics interactions. HPLC was used to determine cell transport samples. The total plasma concentrations of quercetinand isorhamnetin were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) by treatment with β-glucuronidase and sulfatase. Results: The permeability ratio (absorptive permeability/secretive permeability) of isorhamnetin across human MDR1 cDNA transfected MDCKII cells, Caco-2 cells and wild-type MDCKII cells are 0.25±0.02, 0.74±0.05, and 1.41±0.06, respectively. This result proved the role of P-gp in the cell efflux of isorhamnetin. While co-transporting with each other across Caco-2 cells monolayer, the permeability ratio of isorhamnetin and quercetin increased by 4.3 and 2.2 times. After coadministration with each other to rats,the Cmax, AUC0-72h, and AUC0-∞ of both isorhamnetin and quercetin significantly increased compared with single administration. Conclusion: The above results proved intra-herb pharmacokinetics interaction between quercetin and isorhamentin. P-gp might play an important role, whereas other drug efflux pumps, such as multi-drug resistance associate protein 2 and breast cancer resistance protein, might be involved. Accordingly, besides the drug-herb interactions, intra-herb interaction might be brought into view with the wide use of herbal-based remedies.

  11. Demarcation of Seabuckthorn Plantations in Three Northern Areas of China

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Seabuckthorn (Hippophae rhamnoides) planting areas in the three northern areas (north, northeast and northwest) of China are divided into five planting zones: the semi-humid forest prairie climate zone for ecological and economic types of seabuckthorn plantations in the southern part of the Loess Plateau; the semi-arid steppe climate zone for similar types of plantations in the central part of the Loess Plateau; the arid desert steppe climate zone for ecological type of seabuckthorn plantations in the northern part of the Loess Plateau; the semi-arid and semi-humid steppe climate zone again for ecological and economic types of plantations in northern Hebei and western Liaoning and the cold humid steppe climate zone for economic types of plantations in the northern part of northeast China. The aim of this demarcation is to avoid a random introduction of seabuckthorn. In each of the five zones,objectives should be set and suitable seabuckthorn species, subspecies and varieties should be planted according to site conditions,seed sources and methods of tree breeding. The cultivation centers, bases, stations, or units should be established and successful models of seedling and planting methods should be encouraged. The principle of matching trees with suitable site conditions and adjusting measures to local conditions should be practiced. From a strategic viewpoint of solving ecological and economic problems of seabuckthorn development in the three northern areas, every seabuckthorn center must have its own germplasm nursery, standard plantation for popularizing, excellent seed and seedling nurseries and sufficient afforestation areas for demonstration and propaganda purposes. These measures would improve the ecological environment and promote economic and social development in the three northern areas of China.

  12. Characterization of Soil Microbial Community Function and Structure in Rhizosphere of Typical Tree Species and the Meaning for Environmental Indication in the Loess Plateau%黄土高原典型林木根际土壤微生物群落结构与功能特征及其环境指示意义

    Institute of Scientific and Technical Information of China (English)

    张海涵; 唐明; 陈辉

    2009-01-01

    Determination of the soil microbial community structure in rhizosphere of typical tree species in the Loess Plateau can be of great theoretical significance for correctly assessing the characteristics of soil ecological rehabilitation of the Loess Plateau. In this study, spore density analysis, microbial cultivation and BIOLOG were employed to evaluate the AMF spore density and soil microbial community diversity under four tree species with vesicular-arbuscular mycorrhizae in ecological rehabilitation area of the Loess Plateau, north Shaanxi Province. The results show that the different tree species differed significantly in both soil microbial number and microbial functional diversity, AMF spore density of Hippophae rhamnoides soil is 2.24 times than that of the Robinia pseudoacacia soil, and the rank as following order: Hippophae rhamnoides > Sophora viciifolia > Caragana mwrophjlla > Robinia pseudoacacia . The statistical significant are detected in the bacteria and actinomyces numbers, however, there is no statistical significance in fungi number among the treatments. The principle component analyses indicates that scatter of Caragana microphylla and Hippophae rhamnoides axe smaller than that of Sophora viciifolia and Robinia pseudoacacia, these results suggest that the soil community structure strongly varied among the different tree species. Numbers of carbon sources related to the first two components are 14 and 8. Correlation analysis shows that the AMF spore density appeared extremely significantly and positively correlated with the number of bacteria, and the metabolic of amino acids, amines and aromatic compounds, respectively . Moreover, AMF spore density positively correlated with the average well color development (AWCD) , nevertheless, no correlations are found among AMF spore density, carboxylic acids, carbohydrates and polymers. These results suggest that AMF spore density is shown to be an important environmental biology parameter used in

  13. 胡颓子科2种旱生植物次生木质部的生态解剖学特性%Ecological Anatomy Characteristics of Secondary Xylem Cells of Two Xerophytes in Elaeagnaceae

    Institute of Scientific and Technical Information of China (English)

    杨淑敏; 江泽慧; 任海青; 古川郁夫

    2008-01-01

    The wood anatomy of Hippophae rhamnoides and Elaeagnus angustifolia we re described and compared from an ecological perspective. Both species showed si milar wood structure: distinct growth ring boundaries, semi-ring-porosity, sim ple perforation plate, alternate intervessel pitting, non-septate fibres and he lic al thickenings are present while axial parenchyma is absent or rarely present. Typical different characters of two species in Elaeagnaceae are ray type and wi dth and storied structure. In H. Rhamnoides, there is a distinct storied str uctu re in rays, axial parenchyma cells and vessel elements, but irregularly storied in E. angustifolia. Rays are uni-to bi-seriate, heterogeneous in H. Rham n oides, and 2~5-seriate, homogeneous in E. angustifolia. But there were f ew quantitati v e differences observed between them. The vessel frequency is larger, vessel elem ent length is shorter and vessel diameter is much narrower in H. Rhamnoides, thu s lead to a smaller vulnerability and mesomorphy value, indicating adaptive to x eric conditions. The horizontal variation of vessel element and fibre length alo ng the ring number from pith showed irregular tendency. There are significantly difference in vessel element length and fibre length within trees and between sp ecies. Furthermore, the relationships between anatomical features and adaptabili ty to desert environments were discussed.%从生态学角度对沙棘和沙枣的木材结构进行对比研究.两树种具有的共同特点为 :生长轮明显,半环孔材,具单穿孔,导管间纹孔交互排列,无分隔木纤维,导管较窄, 环管管胞和纤维状管胞具螺纹加厚,轴向薄壁组织缺失或很少.所选树种明显区别是射线类型、宽度和叠生排列方式:沙棘射线,轴向薄壁组织细胞和导管分子具有明显的叠生排列方式,但沙枣的叠生排列不规则;沙棘射线为异型,1~2列,沙枣射线为同型,2~5列;并且二者之间数量化指标有差异,沙

  14. Extracting technology of total flavonoid glycosides in fruit of Hippopphae rhamnoides%沙棘果中总黄酮苷类的提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    袁媛; 张浩; 郑苗

    2012-01-01

    OBJECTIVE To find out the best method of extracting total flavonoid glycosides in the berries of seabuckthorn. METHODS Ethanol concentration, extraction temperature, ratio of liquid to solid and extraction time were selected by using single factor experiment and orthogonal experiment. RESULTS The optimum extraction conditions were obtained as extraction temperature of 70 °C , ethanol concentration of 60% , extraction time of 0. 5 h for each time, 3 times in all, ratio of liquid to solid 1: 15. CONCLUSION Total flavonoid aglycones obtained by hydrolyzing was first used as an index in process optimization of extracting flavonoid glycosides. It' s necessary to study the total flavonoids in seabuckthorn further. Meanwhile, we should not only determine the content of total flavonoid aglycones before hydrolyzing, but also determine the content of total flavonoid aglycones after hydrolyzing.%目的 探讨沙棘果实中总黄酮苷类的最佳提取工艺.方法 采用单因素实验和正交实验结合的方法筛选,对乙醇浓度、提取温度、料液比、提取时间进行四因素三水平的正交试验.结果 最佳工艺条件为70℃下采用60%的乙醇浸提,固液比1∶15,回流提取(0.5 h×3).结论 首次将水解后的沙棘总黄酮苷元作为沙棘总黄酮提取工艺的评价指标,在分析检测方法上不仅应测定水解前,还应考察测定水解后总黄酮苷元的含量.

  15. Comparative Anatomical Study on Wood of Three Kinds of Hippophae Stem%3种沙棘茎的木材比较解剖学研究

    Institute of Scientific and Technical Information of China (English)

    张丽娟; 王立军; 聂小兰

    2007-01-01

    对同一生境下的中国沙棘、蒙古沙棘、俄罗斯沙棘茎的木材进行了比较解剖学研究,发现3种沙棘茎的木材均为环孔材,管孔密度蒙古沙棘最大,管孔直径俄罗斯沙棘最大,导管、管胞、木纤维的长度均为蒙古沙棘最长,俄罗斯沙棘导管及木纤维的长度均小于中国沙棘,但中国沙棘的管胞最短.中国沙棘多为多列射线,蒙古沙棘单列射线居多,俄罗斯沙棘无多列射线,这表明3种沙棘不同组织的进化不同步.3种沙棘木纤维长度均大于500μm,符合纤维板的生产要求.薄壁组织的存在可为形成层的活动及不定根、不定芽的产生提供养分.

  16. The effect of host plant species on the development ofTrabala vishnou gigantina Yang (Lepidoptera:Lasiocampidae)%栎黄枯叶蛾生长发育与几种寄主内含物的关系

    Institute of Scientific and Technical Information of China (English)

    温冬梅; 刘永华; 任利利; 陆鹏飞; 骆有庆

    2016-01-01

    impact of different hosts on the population dynamics ofT. vishnou gigantina.[Methods]The pupae weight, fecundity, and the duration of larvae and pupal stages of T. vishnougigantina, reared on four different host plants (Hippophae rhamnoices,Armeniaca sibirica,Populus davidiana,Salix matsudana) under laboratory condition, were compared, and secondary compounds, soluble sugar, soluble protein and water content of the four host plants, determined. The relationship between larval development, adult fecundity, and host plant inclusion contents was analyzed.[Results]Host plant species had a significant effect on the duration of larval and pupal stages, pupal weight, and reproduction.Larvae fed on sea-buckthorn had the shortest larval stage (74.50±1.76) d, and those fed on willow the longest (106.00±1.51) d. Larvae fed on poplar had the longest pupal stage (44.00±1.41) d and those fed on willow the shortest (32.70±3.13) d. Larvae reared on sea-buckthorn, or apricot, had significantly higher pupal weight and adult fecundity than those reared on poplar or willow. Nutrients, water and secondary compounds differed significantly between the different host plants. Soluble sugar content was significantly, negatively correlated with female pupal weight and fecundity, and soluble protein content was significantly negatively correlated with duration of the larval stage. Female and male pupal weights were positively correlated with water content.There was no relationship between flavone content and the duration of the larval stage or adult fecundity, but tannin content was significantly, positively correlated with the duration of pupal stage.[Conclusion] The results indicate that sea-buckthorn was the most suitable of the four host plants forT. vishnou gigantina. The development and adult fecundity ofT. vishnou gigantinaare better when larvae feed on host plants with lower soluble sugar and tannin content, and higher water and soluble protein content.

  17. Effects of Biological Soil Crusts on Seed Emergence and Seedling Growth in Loess Plateau,North Shaanxi Province%黄土高原土壤生物结皮对植物种子出苗和生长的影响

    Institute of Scientific and Technical Information of China (English)

    王蕊; 朱清科; 赵磊磊; 常存; 马浩

    2011-01-01

    Biological soil crusts are prevalent in arid and semiarid regions,they are the complex mixtures of cyanobacteria,green algae,phycolichens,mosses,liverworts,fungi and bacteria.In order to understand the effect of biological soil crusts(BSCs) on seed emergence and seedling growth in loess region,we conducted field surveys and studied the impact of intact crusts,broken crusts and soil on seed emergence of 4 plant species including Pinus tabulaeformis,Caragana korshinskii,Hippophae rhamnoides and Pyrus betulifolia based on the field emergence experiment in Wuqi County,Shaanxi Province during the period from July to August 2009.The results reveal that the vegetation coverage was reduced significantly with the increase of BSCs coverage and thickness(P0.01),and vegetation types were in an increase trend(P0.05).Seed emergence rate and emergence process were affected by plant species and crust types(intact crusts,broken crusts and soil)(P0.01),and the interaction between the two affected slightly the seedling emergence(P0.05),but affected significantly the emergence process(P0.01).The seed emergence rate under broken BSCs was higher than that under intact BSCs,but there was no significant difference with soil.Broken BSCs could increase plant biomass and root-shoot ratio of plants,but intact BSCs affected the plant biomass and root-shoot ratio depending on the types of seed.%为了解生物土壤结皮对植物种子萌发出苗和植物生长的影响,于2009年7~8月在陕西省吴起县进行野外调查,对油松(Pinus tabulaeformis)、柠条(Caragana korshinskii)、沙棘(Hippophae rhamnoides)和杜梨(Pyrus betulae-folia)种子进行萌发实验,研究生物结皮与植被之间的相互关系以及完整结皮、破碎结皮和覆土处理对这4种种子出苗和生长的影响。结果表明:随着生物结皮盖度和厚度的增加,植被盖度和数量显著减少(P〈0.01),植被种类有增加的趋势(P〉0

  18. [Soil organic carbon storage changes with land reclamation under vegetation reconstruction on opencast coal mine dump].

    Science.gov (United States)

    Li, Jun-Chao; Dang, Ting-Hui; Guo, Sheng-Li; Xue, Jiang; Tang, Jun

    2014-10-01

    Vegetation reconstruction was an effective solution to reclaim the opencast coal mine dump which was formed in the process of mining. To understand the impact of the vegetation reconstruction patterns' on the mine soil organic carbon (SOC) storage was essential for selecting the methods of vegetation restoration and also important for accurately estimating the potential of the soil carbon sequestration. The study area was on the Heidaigou opencast coal mine, which was 15 years reclaimed coal mine dump in Zhungeer, Inner Mongolia autonomous region, we selected 5 vegetation reconstruction patterns (natural recovery land, grassland, bush land, mixed woodland of arbor and bush, arbor land), and 16 vegetation types, 408 soil samples (0-100 m), to study the effect of the vegetation reconstruction patterns on the SOC storage. The results were showed as follows: (1) on the reclaimed coal mine dump, the vegetation reconstruction patterns significantly affected the SOC content and its distribution in the soil profile (P shrub land > arbor forest > mixed forest of arbor and shrub > natural recovery land, in which the grassland, shrub land and arbor forest were about 2.2, 1.3, and 1.3 times of natural recovery land (2.14 g · kg(-1)) respectively. The total nitrogen (TN) showed the similar trends. (2) Among the vegetation types, Medicago sativa had the highest surface SOC content (5.71 g · kg(-1)) and TN content (0.49 g · kg(-1)), that were 171.3% and 166.7% higher than the natural recovery land, and two times of Hippophae rhamnoides, Amorpha fruticosa + Pinus tabulaeformis and Robinia pseudoacacia. (3) The effect of vegetation types on SOC mainly concentrated in the 0-20 cm depth, and the effect on TN accounted for 40 cm. (4) For the SOC storage, the order was original landform area > reclaimed dump > new dump and grassland > woodland (including arbor and shrub land). After 15 years revegetation, the soil carbon storage of the grassland, shrub land and arbor land were

  19. [Application of wastewater land treatment technique to the construction of ecological engineering in sand land].

    Science.gov (United States)

    Li, Pei-jun; Wang, Zhi-jiang; Sun, Tie-heng; Tai, Pei-dong; Chang, Shi-jun; Xiong, Xian-zhe; Li, Ying-mei

    2005-05-01

    In this paper studies on the feasibility of harmlessness and resource of wastewater, which was discharged from a thermal power plant, by using slow rate filtration of land treatment technique for the fast recovery of vegetation in the Kubuqi sand land were carried out. The selected arbor, shrub and herbage in the land treatment system were poplar (Populus alba Var. Pyramidalis bunge), seabuckthorn (Hippophae rhamnoides) and sweet clover (Melilotus suaveolens) respectively. Three levels of wastewater hydraulic loading were designed in the field pilot experiment. They were high plot with 3000 mm/a irrigation (H), medium plot with 1500 mm/a irrigation (M) and low plot (L) with small volume of irrigation only used in the period of transplant seedlings. The performance indicate that the purification function of power plant wastewater by pre-treatment through combination of precipitation pool with storage ponds is effective and therefore the effluent after pretreatment can be used to irrigation vegetation. The experiment results show that the volume of tree crown for poplar in H plot and M plot was up to 1.07 and 2.21 times comparing with L plot respectively. The annual yield (dry weight) of sweet clover in H plot and M plot was up to 2.33 and 3.0 times comparing with L plot respectively. The height of seabuckthorn in H zone and M plot was up to 1.08 and 1.32 times comparing with L plot respectively. There is direct proportion between growth status of vegetation and hydraulic loading of irrigation. The contents of heavy metals for sweet clover (Cd 0.021 mg/kg, Pb<0.001 mg/kg, Cr <0.01 mg/kg, As 0.043 mg/kg) are much lower than the food standards of grain and vegetables, therefore the sweet clover for raising livestock is safe. Wastewater in this area is valuable source. Its reasonable utilization can contribute important benefits in economy and ecology in the ecological construction and developing effective agriculture and animal husbandry.

  20. Late Pleniglacial vegetation in eastern-central Europe: are there modern analogues in Siberia?

    Science.gov (United States)

    Magyari, Enikő Katalin; Kuneš, Petr; Jakab, Gusztáv; Sümegi, Pál; Pelánková, Barbora; Schäbitz, Frank; Braun, Mihály; Chytrý, Milan

    2014-07-01

    To characterize Late Pleniglacial (LPG: 26.5-15 ka cal BP) and particularly Last Glacial Maximum (LGM: 21 ± 2 ka cal BP) vegetation and climate, fossil pollen assemblages are often compared with modern pollen assemblages. Given the non-analogue climate of the LPG, a key question is how glacial pollen assemblages and thereby vegetation compare with modern vegetation. In this paper we present three LPG pollen records from the Carpathian Basin and the adjoining Carpathian Mountains to address this question and provide a concise compositional characterization of the LPG vegetation. Fossil pollen assemblages were compared with surface pollen spectra from the Altai-Sayan Mountains in southern Siberia. This area shows many similarities with the LPG vegetation of eastern-central Europe, and has long been considered as its best modern analogue. Ordination and analogue matching were used to characterize vegetation composition and find the best analogues. Our results show that few LPG pollen assemblages have statistically significant analogues in southern Siberia. When analogue pairings occur they suggest the predominance of wet and mesic grasslands and dry steppe in the studied region. Wooded vegetation types (continental and suboceanic hemiboreal forest, continental taiga) appear as significant analogues only in a few cases during the LGM and more frequently after 16 ka cal BP. These results suggest that the LPG landscape of the Carpathian Basin was dominated by dry steppe that occurred outside the river floodplains, while wet and mesic grasslands occurred in the floodplains and on other sites influenced by ground water. Woody vegetation mainly occurred in river valleys, on wet north-facing hillsides, and scattered trees were likely also present on the loess plateaus. The dominant woody species were Larix, Pinus sylvestris, Pinus mugo, Pinus cembra, Picea abies, Betula pendula/pubescens, Betula nana, Juniperus, Hippophaë rhamnoides, Populus, Salix and Alnus. The pollen

  1. Drug: D06734 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available yphus jujuba [TAX:326968] Same as: E00105 Therapeutic category: 5100 Rhamnaceae (buckthorn...08304] Crude Drugs Drugs for Qi Sedative drugs D06734 Jujube seed Crude drugs [BR:br08305] Dicot plants: rosids Rhamnaceae (buckthorn family) D06734 Jujube seed PubChem: 47208385 ...

  2. Environ: E00447 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available CPD:C00089], D-Glucose [CPD:C00031], Fructose [CPD:C01496] Hovenia dulcis [TAX:99292] Rhamnaceae (buckthorn ...family) Hovenia dulcis mature fruit or seed (dried) Crude drugs [BR:br08305] Dicot plants: rosids Rhamnaceae (buckthorn family) E00447 Hovenia dulcis fruit or seed ...

  3. Study on soil nutrition and enzyme activity under different afforestation models in coal waste pile%矸石山不同造林模式对土壤养分及酶活性影响的研究

    Institute of Scientific and Technical Information of China (English)

    刘阳; 刘畅; 邢兆凯; 贾树海; 赵旭炜; 李明; 程利

    2013-01-01

    In this paper, five afforestation models i.e. Robinia pseucdoacacia L., Rhus typhina Nutt, Ulmus pumila L., Hippophae rhamnoides L. and natural restoration land (take as control) were studied respectively. According to soil genesis, soil samples were collected and values of organic matter, available phosphorus, available kalium, pH and enzyme activities of catalase, invertase and urease in each soil sample were investigated. Contrasting with the control, the amount of soil nutrition in the soil horizon of Ah were increased in four afforestation models. The model of Ulmus pumila showed the best improvement effects of organic matter and available phosphorus, and increased 194.8%and 442.9%, respectively. The model of Robinia pseudoacacia and the model of Rhus typhina showed the highest improvement effects of available kalium and alkali hydrolysable respectively and increased 262.0%and 509.2% respectively. Contrasting with the control, the activities of catalase, invertase and urease were all increased in the four vegetation restoration models. The highest activities of catalase and urease in Ah appeared in Rhus typhina moddle, and increased 134.5%and 880.9%respectively. The highest activity of urease appeared in Ulmus pumila moddle and increased 119.1%. Vertically, organic matter, alkali hydrolysable, catalase and urease decrease with the depth of the soil and reached the lowest at C level. The lowest amount of available kalium, available phosphorus and activity of invertase appeared at the Level of AC. There was no particular pattern of pH variation along the vertical shift.%本文以抚顺矸石山立地条件下树龄为10年的刺槐Robinia pseudoacacia L.、火炬Rhus typhina Nutt、白榆Ulmus pumila L.、沙棘Hippophae rhamnoides L.林以及植被自然恢复裸地(CK)5种植被恢复模式为研究对象,按土壤发生学层次进行采样,对土壤有机质、碱解氮、有效磷、速效钾、pH、过氧化氢酶、蔗糖酶和脲酶等指

  4. Effects of Chitosan Clarification on the Phenolic Compositions of Sea Buckthorn Juice%壳聚糖澄清对沙棘果汁酚类成分的影响

    Institute of Scientific and Technical Information of China (English)

    何志勇; 夏文水

    2005-01-01

    通过Folin-Ciocalteu比色法测定和HPLC检测分析,对壳聚糖澄清过程中沙棘果汁总酚含量以及一些酚类成分的变化进行了研究,结果显示:壳聚糖澄清对沙棘果汁中的酚类物质有所影响,总酚含量降低了17.2%,澄清后果汁中的儿茶酸、阿魏酸和香豆酸组分损失,没食子酸降低87%,槲皮素减少了62.5%,绿原酸含量变化不大.

  5. Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的工艺优化%Optimization on determination of total polyphenol content in sea buckthorn wine by Folin-Ciocalteu colorimetric method

    Institute of Scientific and Technical Information of China (English)

    牛广财; 闫公昕; 朱丹; 魏文毅; 李世燕; 刘志明

    2016-01-01

    以没食子酸作为对照品,通过单因素和正交试验,对Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的条件进行优化.结果表明,Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的最优条件为:Folin-Ciocalteu试剂1 mL,蒸馏水5 mL,7.5%Na2 CO3溶液3 mL,在30℃下避光反应1h后,测定其在735 nm处的吸光度,总多酚含量在0~20 μg/mL范围内与吸光度值有良好的线性关系.该测定方法的平均加标回收率为100.64%,精密度,稳定性以及回收率的相对标准偏差(RSD)分别为1.46%,0.22%和2.63%.该方法简便、快速,精密度高,稳定性和重复性好,可用于沙棘酒中总多酚含量的测定.

  6. 黄土高原油松与其他树种枯落叶混合分解对土壤性质的影响%EFFECTS OF DECOMPOSITION OF LEAF LITTERS FROM PINUS TABULAEFORMIS AND OTHER TREES ON SOIL PROPERTIES IN THE LOESS PLATEAU

    Institute of Scientific and Technical Information of China (English)

    李茜; 刘增文; 米彩红; 杜良贞

    2012-01-01

    An indoor experiment was carried out of having leaf litters from Pinus tabulaeformis and from other 10 species of trees decomposed, separately or mixedly to explore effects of the decomposition on soil properties and any synergic or offsetting actions between the two in mixture. It was found that decomposition of the leaf litters separately significantly increased the activities of soil enzymes, like urease, dehydrogenase and phosphatase, and contents of organic matter and available N, but varied sharply in the effect on available P and CEC in the soil. The decomposition of leaf litter from P. tabulaeformis mixed with that from Platycladus orientalis, Populus simonii, Robinia pseudoacacia, or Ulmus pumila, separately showed obvious synergic effects on total soil microbe. In decomposition, its mixture with that from Hippophae rham-noides affected activity of the soil enzyme of phosphatase, synergically, but its mixture with the leaf letter from P. orientalis , Betula platyphylla or U. pumila, separately, did reversely. Its mixture with leaf litters from most of the trees, separately, showed a synergic effect on soil available K content, but, reversely on soil available P content. Its mixture with that from P. simonii,H. rhamnoides or Amorpha fruticosa, separately, displayed a synergic effect on soil organic matter content. In terms of soil properties as a whole in their effects, its mixture with leaf litter from H. rhamnoides, R. pseudoacacia, P. simonii or A. fruticosa, separately, all acted synergically, whereas its mixture with that from P. orientalis, Quercus liao-tungensis,B. platyphylla,Larix principis-rupprechtii, or Caragana microphylla,separately,did reversely.%通过油松及其他10个树种枯落叶单独和混合分解试验,探讨了油松与其他树种枯落叶混合分解对土壤性质的影响及在对土壤性质影响中是否存在相互促进或抑制作用.结果表明:11种枯落叶单独分解均明显提高了土壤脲酶、脱氢酶、

  7. 柠条与沙棘共生固氮菌资源特性对比分析%A Comparative Analysis on Resources Characteristics of Symbiotic Nitrogen Fixation Strains of Peashrub and Hippophae

    Institute of Scientific and Technical Information of China (English)

    白建军; 史清亮

    2008-01-01

    柠条与沙棘两种木本灌木均具有御旱与耐旱性,是山西省半干旱气候区的结瘤植物资源优势,其共生固氮菌Rhizobia与Frankia菌系在山西石灰性土壤中的生态分布广泛,结瘤特征明显,大约每年为该生态区提供10万~20万t的生物氮素,在山西的生态环境建设、水土保持与资源利用方面发挥着较大的生态学效应,具有深入研究和广泛的利用价值.

  8. 反相高效液相色谱法测定沙棘油中维生素E的含量%CONTENT DETERMINATION OF VITAMIN E IN OIL OF FRUCTUS HIPPOPHAE BY REVERSED PHASE-HIGH PERFORMANCE LIQUID CROMATOGRAPHY

    Institute of Scientific and Technical Information of China (English)

    吴虹; 吴健

    2002-01-01

    目的:采用反相高效液相色谱法测定沙棘油中维生素E的含量.方法:C18-ODS柱为色谱柱、甲醇-水(98∶2)为流动相,流速为1.5 ml/min,检测波长:280 nm.结果:线性范围0.9~6.0 μg(r=0.999 8),平均回收率为99.17%,RSD为1.48%(n=5).结论:本法操作简便,准确可靠.

  9. Root structure of slope protection plants in a high-grade highway

    Institute of Scientific and Technical Information of China (English)

    WANG Jing-hong; LI Xing-tao; LIU Xiao-dong; Wang Fei; LIU Yu

    2011-01-01

    Root length and root length density of Lespedeza bicolor,Amorpha fruticosa, and Sea buckthorn were investigated in a country highway-TongSan highway (Tongjiang to Sanya) in Heilongjiang Prov ince, China. The root lengths were divided into five root orders according to Pregizter sequence classification method. Results show that sea buckthorn roots are dominated by coarse roots in the horizontal growth, while L. bicolor has a large proportion of fine roots in vertical conical growth and A. fruticosa is in depth growth. Root length density of L. bicolor in all the root sequences is higher than that of sea buckthorn and A. fruticosa. On the basis of the root structure, it is inferred that L. bicolor roots mainly absorb the surface soil moisture for its normal growth; in contrast,A. fruticosa has good uptake ability to deep soil water. The root structure of sea buckthorn implies that it has a strong drought resistance.

  10. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    purple willow (Salix purpurea). Only the 25% of the interventions was accomplished by the use of secondary plant species, as tamarisk (Tamarix spp.,) blackthorn (Prunus spinosa) , whitethorn (Crataegus spp.), sea-buckthorn (Hipphopae rhamnoides), wild pear (Pyrus pyraster), cottonwood (Populus nigra), eglantine (Rosa spp.), goat-willow (Salix caprea) and cornel (Cornus sanguinea). Better results were achieved with Spanish Broom, a very rural plant that can effectively colonise even poor soils like badlands; as a matter of fact, more than the 75% of the interventions had positive outcomes The efficacy of the consolidation work by the presence of living structures point out an increase of the stability of those interventions older than 4 years, with taking root species present from 54% to 78%. So far, the construction and the reliability of the works have been monitored, in order to capture critical aspects for the success of works and to build a geo-referenced data base of the existing works and their status.

  11. Estimation of biological nitrogen fixation by black locust in short-rotation forests using natural 15N abundance method

    Science.gov (United States)

    Veste, M.; Böhm, C.; Quinckenstein, A.; Freese, D.

    2012-04-01

    .4% (C/N 14.4) in 3 year-old trees, respectively. A higher content of nitrogen was found in leaves of re-sprouted trees with 4.3% (C/N 11.5). The estimated percentage of nitrogen derived from the atmosphere (% NdfA) in black locust was 63% - 83% compared to 56% in seabuckthorn (Hippophaë rhamnoides) and 79% in common broom (Genista scuparia). The annual leaf biomass production of black locust varied between 1325 (2 years old trees) and 2576 kg/ha a (4 years old trees). The estimated leaf nitrogen fixed by Robinia was approx. 30.5 - 59.2 kg/ha a. From the results, we can conclude that the biological nitrogen fixation by Robina is an important factor for the nitrogen balance of short-rotation plantations on nutrient poor-soils.

  12. 皇甫川流域几种主要植物水分生态特征%A study on the water ecology of some dominant plants in Huangfuchuan Basin

    Institute of Scientific and Technical Information of China (English)

    杨劼; 高清竹; 李国强; 金争平

    2004-01-01

    通过在丰水年(1998年)和欠水年(1999年)连续两个生长季对皇甫川流域几种主要植物:沙棘(Hippophae rhamnoides)、沙柳(Salix psammophyla) 、中间锦鸡儿(Caragana intermedia)、杨树(Populus simonii)、油松(Pinus tabulaeformis)、沙打旺(Astragalus adsur gens cv.)、羊柴(Hedysarum leave)、玉米(Zea mays)和百里香(Thymus serpyllum) 等植物生物学特性、蒸腾特征及土壤水分含量的测定及分析,得出以下结果:几种主要植物蒸腾强度平均值由大到小的排列顺序是:杨树>沙柳>沙打旺>羊柴>中间锦鸡儿>玉米>百里香>沙棘>油松;在1998年和1999年,皇甫川流域主要植物蒸腾强度的季节动态基本相同,油松、沙棘、中间锦鸡儿以及百里香等植物年平均蒸腾强度较低.无论丰水年还是欠水年,生长季内皇甫川流域主要植物蒸腾系数均较高,表明了植物的生长是以消耗大量水分为代价,水分是其生长的主要限制因子;百里香蒸腾系数最小,其次是中间锦鸡儿,这反映了百里香和中间锦鸡儿能更有效地利用水分进行干物质积累;皇甫川流域乔木适宜种为油松、灌木种为中间锦鸡儿和沙棘、半灌木植物为百里香.主要植物群落地段的蒸散量与降雨量的比值在1左右.但从土壤水分变化角度看,皇甫川流域主要植物群落地段的土壤水分处于亏缺状态,主要植物群落是在水分胁迫之下维持生长的.在水分平衡条件下,皇甫川流域油松适宜盖度为36%~47%、杨树为37%~48%、中间锦鸡儿为43%~55%、沙柳为46%~59%、沙棘为61%~79%、百里香为68%~87%、沙打旺为50%~65%、羊柴为54%~69%.

  13. Selection of Indigenous Plants in Roads in Northwestern Loess Plateau Area%西北黄土高原地区路域乡土植物资源筛选研究

    Institute of Scientific and Technical Information of China (English)

    张铁军; 王春玲

    2012-01-01

    [ Object] Hie aim was to study on resource utilization of indigenous plants in roads in order to solve resource shortage of indigenous plant in bioengineering. [ Method ] Based on northwestern loess plateau area, we conducted research on selection of indigenous plants and survey on the species which could live in adverse condition, mainly by surveying, with frequent tests as a supplement, to make a comprehensive observation and evaluation on plant combinations which are to be extensively applied. [ Result] The research indicated that in central dividing strips, four arbors, including Platycladus orientnlis (Linn. ) Franco, Sabina chinensis, Sabinachinensis ( Linn. ) Ant. , and Ulmus pumila L. , and nine frutices, including Lycium chinense, Tamarix austromongolica, Rosa xanthina lindl. , Forsythia suspense, Atriplex canescens, Amygdalus triloba, Hippophae rhamnoides Linn. , Amorpha fruticosa L. , and Syzygium aromaticum could be planted. In addition, Gleditsia sinensis Lam, Robinia pseudoacacia L, Elaeagnus angustifolia L. , Rhus typhina, Salix babylonica, Salixmatsudana and Fraxinus chinensU, tall and strong, are suitable to be planted as border trees, presenting a good vision. Frutices, limited by height, are usually with more branches, which will provide a good landscape if grown with tall plants. Prunus dacidiana and Ulmus pumila L. , prone to be damaged by insects, should be planted with other species to stop the infestation in space. [ Conclusion ] The research laid foundation for selection of indigenous plants in roads, providing references for resource shortage of indigenous plant for bioengineering.%[目的]通过对路用乡土植物区资源利用研究,解决生物工程所需的乡土植物资源不足问题.[方法]以西北黄土高原区为研究范围,以调查研究为主、试验研究为辅开展了乡土植物资源筛选的研究工作,调查了该区域能在恶劣条件下生长的植物种类及其在路域的适应状况,对有望大规

  14. Decomposition of different plant litters in Loess Plateau of Northwest China%黄土高原不同植物凋落物的分解特性

    Institute of Scientific and Technical Information of China (English)

    李云; 周建斌; 董燕捷; 夏志敏; 陈竹君

    2012-01-01

    Taking the litters of species Hippophae rhamnoides, Medicago sativa, Populus simonii, Robinia pseudoacaci, Salix psammophila, and Stipa bungeana in the Loess Plateau of Northeast China as test objects, and by using mesh bags, this paper studied the dynamic changes of the litters mass, carbon, and nitrogen during decomposition after buried in the field in semiarid region. The litters buried were from one, two, or three of the plant species, and mixed thoroughly with equal proportion of masses. During decomposition, the mass loss rate, total carbon and nitrogen release rates, and total soluble carbon and nitrogen contents of different litters were higher at the early than at the later decomposition stage. After 412 d decomposition, the average mass loss rate of the litters was in the order of mixed litters of three plant species > mixed litters of two plant species > one plant species litter. By the end of this experiment, the average release rates of the litter total carbon and nitrogen ranked as one plant species litter > mixed litters of two plant species > mixed litters of three plant species, the litter soluble organic carbon content was mixed litters of two plant species > mixed litters of three plant species > one plant species litter, while the litter soluble total nitrogen content was mixed litters of three plant species > mixed litters of two plant species > one plant species litter. Correlation analysis showed that the utter mass loss rate had definite correlation with the litter soluble organic matter, especially soluble organic carbon. From the viewpoint of mass loss rate, the mixture of the litters of P. simonii, H. rhamnoide, and M. sativa was the optimum. It was suggested that in the process of returning farmland into forestland and grassland in the gully and valley region of Loess Plateau, it would be required to rationally increase plant species diversity to improve soil fertility.%以黄土高原区典型植物刺槐、小叶杨、沙棘、沙柳

  15. Effects of applying tree leaf litter on control of soil polarization of continuous cropping wheat land in the Gullied Loess Plateau%利用树木枯落叶防治连作麦田土壤极化的效应

    Institute of Scientific and Technical Information of China (English)

    刘增文; 米彩红; 李茜; 袁娜; 祝振华

    2011-01-01

    【目的】研究不同树木枯落叶对连作麦田土壤性质的影响,为防治麦田土壤性质极化和连作障碍提供依据。【方法】采集黄土残塬区典型连作麦田耕层(0~10cm)土壤,将其与不同森林树种(刺槐、小叶杨、辽东栎、沙棘和柠条)和城市道路绿化树种(法国梧桐、国槐、银杏、垂柳、五角枫、女贞、紫叶李、栾树、广玉兰、梧桐、泡桐、杜仲和七叶树)的当年枯落叶按干质量比100∶2混合后,在20~25℃下进行室内分解培养试验,连续培养120d后,测定土壤的化学性质和生物学性质。【结果】1)连作麦田会引起土壤有效Zn、有机C含量增加和土壤微生物数量、多种酶活性不断提高的正向极化,但也同时引起土壤向贫养化发展、土壤有效Fe和有效Cu含量降低、阳离子交换量(CEC)减少及土壤部分酶活性降低的负向极化趋势;2)根据对连作麦田土壤极化的综合影响效应分析,枯落叶最适合施入麦田土壤的当地森林树种是沙棘、小叶杨,其次是刺槐,而柠条、辽东栎不适宜;枯落叶最适合施入麦田土壤的当地城市道路绿化树种是杜仲、紫叶李,其次是栾树、国槐、垂柳、泡桐,而女贞、法国梧桐、银杏、梧桐、七叶树、玉兰和五角枫均不适宜。【结论】收集一些特定树种的枯落叶施入农田土壤,是防治连作土壤性质极化和连作障碍的一种有效方法。%【Objective】 The study was about the effects of different leaf litters on continuous cropping soil of wheat which can provide scientific basis for controlling soil polarizations and continuous cropping obstacle.【Method】 Through sampling top soil(0-10 cm) of typical wheat land in the gullied Loess Plateau,mixed with different leaf litters of forest trees(Robinia pseudoacia,Populus simonii,Quercus liaotungensis,Hippophae rhamnoides,Caragana microphylla) and urban road trees

  16. 沙棘油在体外对肾虚型再生障碍性贫血作用的实验研究%Experimental research on effect of sea buckthorn oil on marrow hematopoiesis in patients with aplastic anemia of kidney deficiency type in vitro GE

    Institute of Scientific and Technical Information of China (English)

    葛志红; 王栋范; 宾冬梅; 李宜真

    2008-01-01

    目的 观察沙棘油在体外对肾阳虚、肾阴虚不同证型再生障碍性贫血(AA)患者红系集落形成单位(CFU-E)、粒巨噬细胞系集落形成单位(CFU-GM)的影响.方法 20例AA患者(肾阴虚型组及肾阳虚型组各10例),正常对照组10例,取其骨髓分离单个核细胞,以琼脂半固体培养联合沙棘油的方法,培养3、7 d后分别对比各组CFU-E、CFU-GM集落形成情况.结果 不同证型AA患者的CFU-E、CFU-GM较正常对照组明显减低(P<0.01);沙棘油能明显促进不同证型AA患者CFU-E、CFU-GM的集落生成,这种促进作用随沙棘油体积分数增加而增加;在体积分数不低于20%时,沙棘油对肾阳虚患者CFU-E、CFU-GM的疗效明显好于对肾阴虚患者的疗效(P<0.05).结论 沙棘油在体外对AA患者CFU-E、CFU-GM有一定的促增殖作用,且高剂量时对肾阳虚证型患者的作用优于肾阴虚证型患者.

  17. 沙棘酒皮渣发酵提取物对金黄色葡萄球菌的抑制效果的研究%Study on the antibacterial effect of sea buckthorn wine residue fermentation extraction on Staphylococcus aureus

    Institute of Scientific and Technical Information of China (English)

    焦扬; 李彩霞; 高正睿; 刚丽娟; 张爱萍

    2014-01-01

    采用地衣芽孢杆菌发酵沙棘酒皮渣,研究了发酵提取物(SBFE)对金黄色葡萄球菌的抑制效果.结果表明,SBFE对金黄色葡萄球菌的MIC为1.2mg/mL,且SBFE中抑菌成分具有较高的热稳定性,其最佳抑菌pH为7.5.在SBFE中加入大于2%的葡萄糖会降低抑菌效果,但加入大于0.5%的柠檬酸和小于8%的苯甲酸会增加抑菌效果.沙棘酒皮渣发酵液中的抑菌物质可能是多种成分的共同作用,发酵可使多肽、多酚和黄酮等有效抑菌成分的浓度提高,增强了抑菌效果.

  18. Primary study of the preventive effects of allicin, Hippohae rhamnoides oil and ginkgo bible extract on cisplatin-induced nephrotoxicity%大蒜素、沙棘油、银杏叶提取物对顺铂所致大鼠肾损害预防作用的初步研究

    Institute of Scientific and Technical Information of China (English)

    刘勇; 高岩松; 仲来福

    2002-01-01

    目的:研究大蒜素、沙棘油、银杏叶提取物对顺铂(CDDP)所致肾损害的预防作用.方法:将30只SD大鼠随机分5组,分别为对照组(生理盐水组)、CDDP组、CDDP+银杏叶提取物组、CDDP+大蒜素组、CDDP+沙棘油组,进行急性毒性实验,分别测定体重、肾脏系数、血清尿素氮等指标(BUN).结果:CDDP+大蒜素组和CDDP+沙棘油组BUN水平较CDDP组明显降低(P<0.05).结论:大蒜素和沙棘油能减轻CDDP所致肾损害作用.

  19. Maintaining genetic diversity and population panmixia through dispersal and not gene flow in a Holocyclic heteroecious aphid species

    Science.gov (United States)

    Heteroecious holocyclic aphids alternate between sexual and asexual reproduction on primary and secondary hosts, respectively. Most of these aphids are generalists, but the aphid specialist Aphis glycines survives only on the primary host buckthorn (Rhamnus spp.) and the secondary host soybean (Gly...

  20. Influence of Nutrient-release of Mix-leaf Litter Decay of Populus simonii with Other Trees in the Loess Plateau%黄土高原小叶杨与其他树种枯落叶混合分解对养分释放的影响

    Institute of Scientific and Technical Information of China (English)

    祝振华; 刘增文; 袁娜; 杜良贞

    2012-01-01

    Soil polarization resulted from continuous growth and planting of pure forest stands is main cause of soil degradation and unbalanced or unsustainable development of artificial forest ecosystem. Fundamental way of controlling soil polarization is to select friendly tree species to form mixed forests. Indoor mixed decomposition experiment was conducted to study the effect of decomposition of mixed leaves of Populus simonii and other species on nutrient release, and to provide theoretical basis for artificial mixed forest formation. The results were as follows. (1). Quercus liaotungensis leaf litter strongly promoted organic carbon release with a promotion percentage of 21. 32%, while Pinus tabulae formi demonstrated suppression effect, and the release rate decreased by 14. 78%. No significant effects on the organic carbon release were observed among the leaves of other tree species. (2) Q. liaotungensis leaf litter strongly increased nitrogen release with a promotion rate of 28. 69%, showing significant promotion effect, for P. sylvestris var. mongolice and Robinia pseudoacacia leaf litters, however, the promotion effects were relatively weaker and nitrogen release rate increased by 14. 98% and 13. 36% respectively. P. Tabulaeformis leaf litter ex-hibited strong inhabiting effect on nitrogen release, the release rate decreased by 20. 33%. For the leaves of other tree species, no significant effects on nitrogen release were observed. (3) R. pseudoacacia, Q. li-aotungensis, and Hippophae rhamnoides leaf litters very strongly promoted the phosphorus release rate with promotion percentages of 57. 60% , 43. 06% , and 38. 60% respectively. Amorpha fruticosa leaf litter strongly promoted phosphorus release, the promotion rate increased by 22. 64%. P. tabulae formis and P. sylvestris var. mongolica leaf litters significantly inhabited phosphorus release, the release rate reduced by 62. 63% and 47. 99%, respectively. The leaf litters of other tree species showed no

  1. Effects of mixed decomposition of Populus simonii and other tree species leaf litters on soil properties in Loess Plateau%黄土高原小叶杨与其他树种枯落叶混合分解对土壤性质的影响

    Institute of Scientific and Technical Information of China (English)

    李茜; 刘增文; 杜良贞

    2012-01-01

    pseudoacacia, or Ulmus pumila showed interactive promotion effects on the abundance of soil microbes, and that of the mixed leaf litters of P. simonii and P. orientalis or C. microphylla showed interactive promotion effects on the soil organic matter, available P, and available K contents and soil CEC but interactive inhibition effects on the activities of most of the soil enzymes tested. The decomposition of the mixed leaf litters of P. simonii and Larix principis-rupprechtii showed interactive promotion effects on the activities of most of the soil enzymes and soil nutrient contents, while that of the mixed leaf litters of P. simonii and P. sylvestris var. mongolica showed interactive inhibition effects. Overall, the decomposition of the mixed leaf litters of P. simo- nii and U. pumila, P. tabulaeformis, L. principis-rupprechtii, or R. pseudoacacia could improve soil quality, but the mixed leaf litters of P. simonii and P. orientalis, C. microphylla, P. sylvestris var. mongolica, Hippophae rhamnoides, or A. fruticosa showed an interactive inhibition effect during their decomposition.

  2. 不同复垦模式及复垦年限对土壤微生物的影响%Effects of different vegetation restoration patterns and reclamation years on microbes in reclaimed soil

    Institute of Scientific and Technical Information of China (English)

    樊文华; 白中科; 李慧峰; 乔俊耀; 许建伟

    2011-01-01

    the least among composition of three communities of soil microbes. Different types of vegetation restoration were different in improving soil microbes. After 7 years of reclamation, total amounts of microbes in reclaimed soil with different vegetation restoration modes was Amorpha fruticosa>Elaeagnus angustifolia >Hippophae rhamnoides. After 13 years of reclamation, the ecological benefit of different mode were Picea meyeri×Pinus tabulaeformis×Larix principis-rupprechii> Robinia pseudoacacia ×Pinus tabulaeformis × Ulmus pumila> Robinia pseudoacacia×Caragana korshinskii>Agropyron cristatumn × Robinia pseudoacacia×Caragana korshinskii by evaluation of soil microbes. The correlation analysis showed that soil bacterium and microorganism total quantity were obviously positive correlation with soil organic matter and alkali-hydrolysable nitrogen.

  3. The antioxidant activity of selected Romanian honeys

    Directory of Open Access Journals (Sweden)

    Irina DOBRE

    2010-12-01

    Full Text Available Four types of Apis mellifera honey collected in the eastern region of Romania were screened for their total phenolic content by the Folin-Ciocalteau method, for anti radical power as assessed by DPPHradical scavenging assay, for the FRAP assay-ferric reducing antioxidant power. The antioxidant effect over a substrate sensitive to lipid per oxidation and also the presence of some pigments with antioxidant action in honey-ABS 450 were evaluated. All assays revealed the following order of the obtained values: Lime honey>poly-flower honey>Sea buckthorn honey>Acacia honey.

  4. [Anaerobic solid-phase fermentation of plant substrates by Bacillus subtilis].

    Science.gov (United States)

    Ushakova, N A; Brodskiĭ, E S; Kozlova, A A; Nifatov, A V

    2009-01-01

    Solid-phase growth of Bacillus subtilis 8130 on cellulose-rich plant substrates (presscakes or pulp) under hypoxic conditions was accompanied by cellulose depolymerization, protein hydrolysis, and degradation of other plant components, including some processes of mixed-type carbohydrate fermentation. The bacterial fermentation yielded propionic, butyric, and hexanoic acids and butyric acid derivatives. The bacterial metabolism and fermentation degree can be characterized by the proportions of fatty acids in the reaction mixture. The product of sea buckthorn cake fermentation has a good sorption quality.

  5. Woody invasions of urban trails and the changing face of urban forests in the great plains, USA

    Science.gov (United States)

    Nemec, K.T.; Allen, C.R.; Alai, A.; Clements, G.; Kessler, A.C.; Kinsell, T.; Major, A.; Stephen, B.J.

    2011-01-01

    Corridors such as roads and trails can facilitate invasions by non-native plant species. The open, disturbed habitat associated with corridors provides favorable growing conditions for many non-native plant species. Bike trails are a corridor system common to many urban areas that have not been studied for their potential role in plant invasions. We sampled five linear segments of urban forest along bike trails in Lincoln, Nebraska to assess the invasion of woody non-native species relative to corridors and to assess the composition of these urban forests. The most abundant plant species were generally native species, but five non-native species were also present: white mulberry (Morus alba), common buckthorn (Rhamnus cathartica), tree-of-heaven (Ailanthus altissima), honeysuckle (Lonicera spp.) and elm (Ulmus spp.). The distribution of two of the woody species sampled, common buckthorn and honeysuckle, significantly decreased with increasing distance from a source patch of vegetation (P = 0.031 and 0.030). These linear habitats are being invaded by non-native tree and shrub species, which may change the structure of these urban forest corridors. If non-native woody plant species become abundant in the future, they may homogenize the plant community and reduce native biodiversity in these areas. ?? 2011 American Midland Naturalist.

  6. Surface-enhanced Raman spectroscopy studies of yellow organic dyestuffs and lake pigments in oil paint.

    Science.gov (United States)

    Mayhew, Hannah E; Fabian, David M; Svoboda, Shelley A; Wustholz, Kristin L

    2013-08-21

    Identifying natural, organic dyes and pigments is important for the conservation, preservation, and historical interpretation of works of art. Although previous SERS studies have demonstrated high sensitivity and selectivity for red lake pigments using various pretreatment conditions, corresponding investigations of yellow lake pigments and paints are relatively sparse. Here, surface-enhanced Raman scattering (SERS) spectroscopy is used to identify a variety of yellow organic dyestuffs and lake pigments in oil paint. High-quality SERS spectra of yellow dyestuffs (i.e., turmeric, old fustic, Buckthorn berries) and corresponding paints could be obtained with or without sample pretreatment using microliter quantities of HCl and methanol at room temperature. However, the SERS spectra of yellow lake pigments (i.e., Stil de Grain, Reseda lake) and their corresponding oil paints were only observed upon sample pretreatment. Ultimately, we demonstrate a reliable sample treatment protocol for SERS-based identification of turmeric, old fustic, Buckthorn berries, Stil de Grain, and Reseda lake as well as for microscopic samples of the corresponding oil paints.

  7. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage.

    Science.gov (United States)

    Istrati, D; Lacatusu, I; Bordei, N; Badea, G; Oprea, O; Stefan, L M; Stan, R; Badea, N; Meghea, A

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to -45mV) and displayed average sizes of 70nm to 140nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract-bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α.

  8. [Comparison of soil fertility among open-pit mine reclaimed lands in Antaibao regenerated with different vegetation types].

    Science.gov (United States)

    Wang, Xiang; Li, Jin-chuan; Yue, Jian-ying; Zhou, Xiao-mei; Guo, Chun-yan; Lu, Ning; Wang, Yu-hong; Yang, Sheng-quan

    2013-09-01

    Re-vegetation is mainly applied into regeneration in opencast mine to improve the soil quality. It is very important to choose feasible vegetation types for soil restoration. In this study, three typical forest restoration types were studied at Antaibao mine, namely, Medicago sativa, mixed forests Pinus taebelaefolius-Robinia pseudoacacia-Caragana korshinskii and Elaeagnus angustifolia-Robinia pseudoacacia-Caragana korshinskii-Hipophae rhamnoides, to determine the nutrient contents and enzyme activities in different soil layers. The results showed that re-vegetation markedly increased soil nutrient contents and the enzyme activities during the restoration process. The nutrient content of soil in the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest field was significantly higher than those in other plots. It was found that the soil of the P. taebelaefolius-R. pseudoacacia-C. korshinskii mixed forest had the highest integrated fertility index values. In conclusion, the restoration effects of the P. zaebelaefolius-R. pseudoacacia-C. Korshinskii mixed forest was better than that of E. angustifolia-R. pseudoacacia-C. korshinskii-H. rhamnoides, while M. sativa grassland had the least effect.

  9. Effect of wood ash and K-fertilization on {sup 137}Cs uptake by selected forest plants

    Energy Technology Data Exchange (ETDEWEB)

    Mandro, Yrii N. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Rosen, Klas [Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden); Vinichuk, Mykhailo M. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden)

    2014-07-01

    Accumulation {sup 137}Cs by different forest plants and trees after fertilization of soil with potassium and wood ash ({sup 137}Cs-contaminated and non-contaminated) in forest ecosystems of Ukraine contaminated by radionuclides after Chernobyl accident in 1986 was studied. Experiment is performed in Bazar forestry, Zhytomyr region, Ukraine, located about 70 km (51 deg. 5'35'' N, 29 deg. 18'56'' E) from Chernobyl NPP. Potassium fertilizer (KCl, wooden ash (Ash), and {sup 137}Cs-contaminated ash ({sup 137}CsAsh) in different combinations) were spread on the forest floor in April 2012 at a rate corresponding 100 kg/ha potassium. The experiment layout was as follows: 1- Control (no fertilizers were applied), 2- KCl, 3- Ash + KCl, 4- Ash + {sup 137}CsAsh, 5- Ash, 6- {sup 137}CsAsh + KCl. Samples (leaves and annual shoots) of blueberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), rowan (Sorbus aucuparia), birch (Betula), buckthorn (Frangula) and oak (Quercus robur) and also mushrooms (fruit bodies of Russula, Lactarius, Cantharellus, Collybia etc.) and berries (blueberry and cowberry) were taken monthly from each treatment. Samples were measured for {sup 137}Cs with calibrated HPGe detectors. The results of the first year studies show variation of Transfer Factors (TF) for different plants and for the same plants on different treatments. The effect of fertilization was found for blueberry on Ash-applicated (TF = 0.0085 ± 0.0025), {sup 137}CsAsh + KCl-applicated (TF = 0.0105 ± 0.0060) and Ash + KCl-applicated (TF = 0.0123 ± 0.0058) treatments compared to Control (TF = 0.0163 ± 0.0092). Also good effect for rowan on Ash + KCl-applicated treatment (TF = 0.0067 ± 0.0024) compared to Control (TF = 0.0100 ± 0.0064). Effect was less obvious for birch on Ash + KCl-applicated treatment and for cowberry on Ash + KCl-applicated treatment. There was not found an obvious effect of fertilization for buckthorn. Positive effect of

  10. ANATOMIC INVESTIGATION OF HUNGARY'S COMMON SHRUB SPECIES

    Directory of Open Access Journals (Sweden)

    Eszter ANTALFI

    2015-09-01

    Full Text Available In Hungary a huge part of wooden plants are shrubs. Flora of hungarian forests is among the richest in Europe. Many plants can be classified as shrubs or trees as well, circumstances during their development define what they will become. The diverse world of shrubs and weeds delights the eye under 20-30 meter high trees. From these there are some well known which basically everybody recognises is lilac (Syringa vulgaris, elderberry (Sambucus nigra, dog-rose (Rosa canina, single-seeded hawthorn (Crataegus monogyna and common buckthorn (Rhamnus cathartica. To get these species better known – and occasionally foreshadowing their wood industry usage in some way – it is expendient to familiarize ourselves with their microscopic structure and characteristics. Nowadays there are several imaging methods known, however for examining floral tissue the optical microscope is still the most common one to be used.

  11. Optimization of the derivatization protocol of pentacyclic triterpenes prior to their gas chromatography-mass spectrometry analysis in plant extracts.

    Science.gov (United States)

    Jemmali, Zaineb; Chartier, Agnes; Dufresne, Christelle; Elfakir, Claire

    2016-01-15

    This paper focuses on the application of a two-level full factorial design to optimize the key derivatization step before the GC-FID and GC-MS analysis of pentacyclic triterpenes. The derivatization reaction was screened for influential factors and statistically significant parameters with a p value less than 0.05. A multi-response optimization based on a desirability function was then applied, while simultaneously considering overall detection enhancement of compounds. Results showed that derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) in pyridine (22:13:65v/v/v) for 2h at 30°C was the most efficient method of derivatizing all the hydroxyl and carboxylic acid groups contained in the triterpene structures. The validity of the method was demonstrated using GC-MS analyzes of a mixture containing eleven standards (β-amyrin, α-amyrin, lupeol, erythrodiol, uvaol, betulin, oleanolic acid, betulinic acid, ursolic acid, maslinic acid and corosolic acid). These compounds are representative of different classes of terpene compounds bearing different functional groups such as alcohols, diols, and carboxylic acids. The derivatization procedure was then tested on four plant extracts: apple pomace, salvia sclarea (dried leaves and flowers), sea buckthorn (Hyppophae rhammnoides L.) berries, and B. serrata resin. The identification of triterpenes was based on the comparison of their retention time and mass spectra to those of standards. The presence of compounds already identified in the literature was confirmed and new ones such as maslinic and corosolic acids were identified in apples, sea buckthorn and salvia sclarea.

  12. Differential effects of weather and natural enemies on coexisting aphid populations.

    Science.gov (United States)

    Alyokhin, Andrei; Drummond, Francis A; Sewell, Gary; Storch, Richard H

    2011-06-01

    Study of mechanisms responsible for regulating populations of living organisms is essential for a better comprehension of the structure of biological communities and evolutionary forces in nature. Aphids (Hemiptera: Sternorrhyncha) comprise a large and economically important group of phytophagous insects distributed worldwide. Previous studies determined that density-dependent mechanisms play an important role in regulating their populations. However, only a few of those studies identified specific factors responsible for the observed regulation. Time series data used in this study originated from the untreated control plots that were a part of potato (Solanum tuberosum L.) insecticide trials in northern Maine from 1971 to 2004. The data set contained information on population densities of three potato-colonizing aphid species (buckthorn aphid, Aphis nasturtii; potato aphid, Macrosiphum euphorbiae; and green peach aphid, Myzus persicae) and their natural enemies. We used path analysis to explore effects of weather and natural enemies on the intrinsic growth rates of aphid populations. Weather factors considered in our analyses contributed to the regulation of aphid populations, either directly or through natural enemies. However, direct weather effects were in most cases detectable only at P ≤ 0.10. Potato aphids were negatively affected by both fungal disease and predators, although buckthorn aphids were negatively affected by predators only. Parasitoids did not have a noticeable effect on the growth of any of the three aphid species. Growth of green peach aphid populations was negatively influenced by interspecific interactions with the other two aphid species. Differential population regulation mechanisms detected in the current study might at least partially explain coexistence of three ecologically similar aphid species sharing the same host plant.

  13. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage

    Energy Technology Data Exchange (ETDEWEB)

    Istrati, D.; Lacatusu, I.; Bordei, N.; Badea, G.; Oprea, O. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Stefan, L.M. [National Institute of Research and Development for Biological Sciences, Splaiul Independentei Street No. 296, 060031 Bucharest (Romania); Stan, R. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Badea, N., E-mail: nicoleta.badea@gmail.com [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania); Meghea, A. [University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest (Romania)

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to − 45 mV) and displayed average sizes of 70 nm to 140 nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract–bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. - Highlights: • Safety phyto-mediated nanostructured carriers (NLC) based on two kinds of bioactives • Carrot extract incorporation into nanostructured carriers ranged from 78 to 88.3%. • High antioxidant activity of NLC by scavenging up to 98% free oxygen radicals • Extract entrapment represents a viable way for an equilibrated release of carotenoids. • Remarkable regenerative effect of L929 cell, with a proliferation of 133.4%.

  14. HISTOLOGICAL MODIFICATION AT THE JEJUNUM LEVEL GENERATED BY INTRODUCING MEDICINAL PLANTS AND ESSENTIAL OILS IN BROILERS FEED

    Directory of Open Access Journals (Sweden)

    LAVINIA ŞTEF

    2013-12-01

    Full Text Available Medicinal plants and extracts including in monogastric animals feeding represents a current practice because phyto-additives can represent an alternative to antibiotics using. In this way, in Nutrition and animals feeding discipline was been performed an experiment on 6 weeks, respectively from hatching to 42 days of age, on 120 broiler chickens, divided on three experimental variants (LEU, LEP and LM with 40 individuals each of them. The used hybrid was Ross 308. In LEU group were incorporated essential oils of Coriandri fructus, Satureja hortensis, Hipophae rhamnoides, 250 mg at 1 kg combined fodder. In LEP group were included in combined fodder structure a plants premix (Mentha piperita, Salvia officinalis, Melissa officinalis in 2% proportion.. Microscopic studies showed, in the case of experimental groups, a hypertropic process of intestinal mucous membrane, emphasized by villousities and glandular apparatus development, through capilar system extending and leucocytar infiltrate development on all mucous chorion thickness.

  15. [Construction of Frankia genomic libraries and isolation of clones homologous to nodulation genes from Rhizobium leguminosarum].

    Science.gov (United States)

    Cui, Y H; Qin, M; Wang, Y L; Ding, J; Ma, Q S

    1990-01-01

    High molecular genomic DNAs were isolated by using the lysozyme plus achromopeptidase system from Frankia strains At4, Ccol and Hr16, the root nodule endophytes of Alnus, Casuarina and Hippophae respectively, and used to construct genomic libraries in pLAFR1, a broad host range cosmid vector within many gram-negative hosts. The genomic libraries were screened by in situ colony hybridization to identify clones homologous to common nodulation genes of Rhizobium leguminosarum, based on the sequence homology of EcoRI-digested Frankia total DNA to nodABC from Rhizobium meliloti. Several clones showing relatively strong hybridization were found, the recombinant plasmid was isolated, and their homology with Rhizobium nodulation genes was confirmed by spot hybridization. Further work on these positive clones is now underway.

  16. Palynological investigation of a Holocene profile section from the Palaeo-Gaxun-Nur-Basin

    Institute of Scientific and Technical Information of China (English)

    D. Demske; S. Mischke

    2003-01-01

    Palynological investigations on lacustrine sediment samples revealed ageneral dominance of open Artemisia-Chenopodiaceae vegetation in the Palaeo-Gaxun- Nur-Basin (Inner Mongolia) between 5250 and 3500 cal. aBP. Riverside woods (Hippophae, Populus) developed between 3500-3250 cal. aBP and reflect moister growing conditions. During the following moist-dry transition pronounced cooling occurred around 3000 cal. aBP, in upper elevations favouring the spread of montanewoods (Picea, Betula). Aridity strongly increased from 2900 to 2700 cal. aBP. The recorded vegetational and environmental changes after 3500 cal. aBP can be correlated with glacier advances in the mountains and with a humid phase in the Tengger Shamo followed by desertification.

  17. Primeros resultados de la caracterización botánica de mieles producidas por Tetragonisca angustula (Apidae, Meliponinae en Los Naranjos, Salta, Argentina First results of botanical characterization of honeys produced by Tetragonisca angustula (Apidae, Meliponinae in Los Naranjos, Salta, Argentina

    Directory of Open Access Journals (Sweden)

    F. F Flores

    2010-06-01

    Full Text Available Se realizó el análisis melisopalinológico de diez muestras de miel de Tetragonisca angustula Latreille criadas en la localidad Los Naranjos (S 23° 07' 19,2"; O 64° 40' 32,6", Salta, Argentina. Se reconoció un total de 49 tipos polínicos. En las mieles monoflorales, los tipos dominantes correspondieron a: Anadenanthera colubrina, Baccharis, Citrus sp., Myrtaceae nativas y un tipo indeterminado (tipo 6. Las restantes muestras fueron multiflorales. El 62 % de los tipos polínicos corresponde a representantes de la Selva Montana de Yungas; tipos como Anadenanthera colubrina, Bocconia integrifolia, Parapiptadenia excelsa, Phyllostylon rhamnoides, Sapium haematospermum, tipo Myrsine y un conjunto de Myrtaceae nativas que constituyen la primera cita como plantas nectaríferas para T. angustula. También se identificó polen proveniente de plantas típicas de ambientes antropizados como Carica papaya, Citrus sp., Eucalyptus sp., Leonurus sibiricus, entre otros. Estos resultados constituyen una primera aproximación al conocimiento de la flora utilizada por T. angustula y al origen geográfico de sus mieles, estableciendo el primer registro para Selva Montana de Yungas.The melissopalynological analysis was performed on ten honeys samples of Tetragonisca angustula Latreille breeding in the locality Los Naranjos (S 23° 07' 19,2"; W 64° 40' 32,6", Salta, Argentina. We found a whole of 49 pollen types. In the honey monofloral, the dominant types corresponded to: Anadenanthera colubrina, Baccharis, Citrus sp., native Myrtaceae and a type undetermined (type 6. The remaining samples were multifloral. 62% of pollen types corresponding to representatives of the Yungas mountain forest; types like Anadenanthera colubrina, Bocconia integrifolia, Parapiptadenia excelsa, Phyllostylon rhamnoides, Sapium haematospermum, type Myrsine and a set of native Myrtaceae, which constitute the first appointment as nectar sources for T. angustula. Also identified pollen

  18. Effect of the combination of natural antioxidants and packaging methods on quality of pork patties during storage.

    Science.gov (United States)

    Kumar, Vikas; Chatli, Manish K; Wagh, Rajesh V; Mehta, Nitin; Kumar, Pavan

    2015-10-01

    The effect of combination of phyto-extracts (sea buckthorn extract (SBTE), grape seed extract (GSE)) on different physico-chemical, oxidative stability, instrumental colour and texture, sensory and microbiological properties of pork patties were investigated under aerobic and MAP (50 % CO2: 50 % N2) packaging conditions. Treatments viz. T-1 (aerobic packaged control), T-2 (aerobic packaged containing 0.3 % SBTE+ 0.1 % GSE), T-3 (MAP packaged control) and T-4 (MAP packaged containing 0.3 % SBTE+ 0.1 % GSE) at 4 ± 1 °C for 35 days and samples were drawn at 7 days interval. The pH decreased initially up to 21 days and thereafter increased on further storage whereas water activity followed a decreasing trend throughout the storage period, irrespective of the treatment and packaging conditions. Results of oxidative stability parameters revealed that peroxide value, TBARS and FFA followed an increasing trend in both the packaging groups during storage however, the rate of increase was significantly lower (P Standard plate count, psychrophiles and Coliforms were significantly (P count was better maintained in MAP than aerobic condition. Results concluded that the combined use of antioxidants and MAP packaging would be a useful method to control the oxidative and microbial quality changes of pork patties and it can be successfully stored for 35 days.

  19. Acceptance of Nordic snack bars in children aged 8–11 years

    Directory of Open Access Journals (Sweden)

    Anna Holmer

    2012-04-01

    Full Text Available Background: A health promoting diet is suggested to be tailored to regional circumstances to preserve the cultural diversity in eating habits, as well as contribute to more environmentally friendly eating. It may influence consumer acceptance, however, if the components of the diet differs considerably from their habitual food. Objective: This study aimed to investigate whether snack bars composed of Nordic ingredients were accepted by 8–11 year-old Danish (n=134 and Swedish (n=109 children.Design: A seven-point hedonic scale was used to measure the children's acceptance of five snack bars that varied in their composition of whole grains, berries and nuts. A preference rank ordering of the five bars was also performed. Results: The results showed that samples that were rated highest in liking and were most preferred in both countries were a kamut/pumpkin bar and an oat/cranberry bar. The sample with the lowest rating that was also least preferred was a pumpernickel/sea buckthorn bar. Flavour was the most important determinant of overall liking followed by texture, odour and appearance. Conclusions: Children's acceptances and preferences were highly influenced by the sensory characteristics of the bars, mainly flavour. In agreement with earlier studies, the novel food ingredients seemed to influence children's preferences. The Nordic snack bars may have a potential to be a snack option for Danish and Swedish school children, but repeated exposures to the products are recommended to increase children's acceptance.

  20. Comprehensive treatment of generalized parodontitis.

    Science.gov (United States)

    Mdinaradze, N

    2006-06-01

    The complexity of pathogenesis of periodontitis makes the use of comprehensive treatment necessary. We observed 60 patients, 20 women and 40 men among them aged from 20 to 50. In the course of parodontitis, we determined the intensity of inflammatory-destructive changes (Pi) in the course of periodontitis, the degree of fang denudation, the depth of gum and parodontal recesses, degree of the teeth becoming loose; we conducted the microbiological study of the oral cavity microflora and drew up an antibiotic graph. We included laser therapy in the comprehensive treatment course of generalized parodontitis. We divided the patients into two groups. The patients of the first group were treated in two stages. We used peridex, composed of chlorhexidine for antiseptic treatment of oral cavity. We used a combined solution of sage, eucalyptus, camomile and calendula to irrigate the oral cavity. At the second stage we used ointment applications with the composition of 3% indometacin, heparin, vitamins, sea-buckthorn oil and antibiotics. In the course of comprehensive treatment of the patients of the II group we included laser therapy. Our interest in the laser beam, as in the means of parodontitis treatment was stimulated by a number of properties of the laser beam and namely, its anti-inflammatory, desensitizing and antibacterial action promotes intensifying the reparation processes and does not entail any complications. The mentioned method has produced the best therapeutic results and has resulted in the reduced treatment period.

  1. A review of characterization of tocotrienols from plant oils and foods.

    Science.gov (United States)

    Ahsan, Haseeb; Ahad, Amjid; Siddiqui, Waseem A

    2015-04-01

    Tocotrienols, members of the vitamin E family, are natural compounds found in a number of vegetable oils, wheat germ, barley and certain types of nuts and grains. Vegetable oils provide the best sources of these vitamin E forms, particularly palm oil and rice bran oil contain higher amounts of tocotrienols. Other sources of tocotrienols include grape fruit seed oil, oats, hazelnuts, maize, olive oil, buckthorn berry, rye, flax seed oil, poppy seed oil and sunflower oil. Tocotrienols are of four types, viz. alpha (α), beta (β), gamma (γ) and delta (δ). Unlike tocopherols, tocotrienols are unsaturated and possess an isoprenoid side chain. A number of researchers have developed methods for the extraction, analysis, identification and quantification of different types of vitamin E compounds. This article constitutes an in-depth review of the chemistry and extraction of the unsaturated vitamin E derivatives, tocotrienols, from various sources using different methods. This review article lists the different techniques that are used in the characterization and purification of tocotrienols such as soxhlet and solid-liquid extractions, saponification method, chromatography (thin layer, column chromatography, gas chromatography, supercritical fluid, high performance), capillary electrochromatography and mass spectrometry. Some of the methods described were able to identify one form or type while others could analyse all the analogues of tocotrienol molecules. Hence, this article will be helpful in understanding the various methods used in the characterization of this lesser known vitamin E variant.

  2. Evaluation of Beeswax Influence on Physical Properties of Lipstick Using Instrumental and Sensory Methods

    Directory of Open Access Journals (Sweden)

    Giedre Kasparaviciene

    2016-01-01

    Full Text Available The aim of this study was to optimize the lipsticks formulation according to the physical properties and sensory attributes and investigate the relationship between instrumental and sensory analyses and evaluate the influence of the main ingredients, beeswax and oil, with analysis of lipsticks properties. Central composite design was used to optimize the mixture of oils and beeswax and cocoa butter for formulation of lipsticks. Antioxidant activity was evaluated by DPPH free radical scavenging method spectrophotometrically. Physical properties of lipsticks melting point were determined in a glass tube; the hardness was investigated with texture analyzer. Sensory analysis was performed with untrained volunteers. The optimized mixture of sea buckthorn oil and grapeseed oil mixture ratio 13.96 : 6.18 showed the highest antioxidative activity (70±0.84% and was chosen for lipstick formulation. According to the sensory and instrumental analysis results, optimal ingredients amounts for the lipstick were calculated: 57.67% mixture of oils, 19.58% beeswax, and 22.75% cocoa butter. Experimentally designed and optimized lipstick formulation had good physical properties and high scored sensory evaluation. Correlation analysis showed a significant relationship between sensory and instrumental evaluations.

  3. Evaluation of Beeswax Influence on Physical Properties of Lipstick Using Instrumental and Sensory Methods.

    Science.gov (United States)

    Kasparaviciene, Giedre; Savickas, Arunas; Kalveniene, Zenona; Velziene, Saule; Kubiliene, Loreta; Bernatoniene, Jurga

    2016-01-01

    The aim of this study was to optimize the lipsticks formulation according to the physical properties and sensory attributes and investigate the relationship between instrumental and sensory analyses and evaluate the influence of the main ingredients, beeswax and oil, with analysis of lipsticks properties. Central composite design was used to optimize the mixture of oils and beeswax and cocoa butter for formulation of lipsticks. Antioxidant activity was evaluated by DPPH free radical scavenging method spectrophotometrically. Physical properties of lipsticks melting point were determined in a glass tube; the hardness was investigated with texture analyzer. Sensory analysis was performed with untrained volunteers. The optimized mixture of sea buckthorn oil and grapeseed oil mixture ratio 13.96 : 6.18 showed the highest antioxidative activity (70 ± 0.84%) and was chosen for lipstick formulation. According to the sensory and instrumental analysis results, optimal ingredients amounts for the lipstick were calculated: 57.67% mixture of oils, 19.58% beeswax, and 22.75% cocoa butter. Experimentally designed and optimized lipstick formulation had good physical properties and high scored sensory evaluation. Correlation analysis showed a significant relationship between sensory and instrumental evaluations.

  4. Proposition de classement des sources végétales d’acides gras en fonction de leur profil nutritionnel

    Directory of Open Access Journals (Sweden)

    Dubois Virginie

    2008-01-01

    Full Text Available The role of isolated fatty acids on human health has long been studied by the nutritionists. The consequence of this research for the food industry was a need for a better control of the lipidic profile of manufactured food products. The purpose of the present paper was to give the nutritional profile of 53 vegetable oils in relation with their fatty acid composition and their related nutritional potential. In a first section, the role of isolated fatty acids on blood lipids (LDL-Cholesterol, HDL-Cholesterol and triacylglycerols has been reviewed. In a second section, the vegetable oils are gathered in relation with their similar fatty acids composition, with the aim of classifying their properties in accordance with their role on blood lipids. From a nutritional point of view, besides the major α-linolenic acid source that is rapeseed oil, the classification makes some interesting sources to stand out. They contain either mainly α-linolenic acid (camelin, linseed and perilla or both essential fatty acids as α-linolenic and linoleic acids (purslane, chia, salicorna and sea buckthorn seeds. Moreover, this collection is an interesting tool for new designed food products with a better lipid composition.

  5. Polyphenols-rich natural products for treatment of diabetes.

    Science.gov (United States)

    Dragan, S; Andrica, F; Serban, Maria-Corina; Timar, R

    2015-01-01

    Currently, experimental and clinical evidences showed that polyphenols-rich natural products, like nutraceuticals and food supplements, may offer unique treatment modalities in type 2 diabetes mellitus (DM), due to their biological properties. Natural products modulate the carbohydrate metabolism by various mechanisms, such as restoring beta-cells integrity and physiology, enhancing insulin releasing activity, and the glucose using. Sea buckthorn berries, red grapes, bilberries, chokeberries and popular drinks like cocoa, coffee and green tea are all rich in polyphenols and may decrease the insulin response, offer in g a natural alternative of treatment in diabetes. Therefore, researches are now focused on potential efficacies of different types of polyphenols, including flavonoids, phenolic acids, lignans, anthocyans and stilbenes. Animal and human studies showed that polyphenols modulate carbohydrate and lipid metabolism, decrease glycemia and insulin resistance, increase lipid metabolism and optimize oxidative stress and inflammatory processes. It is important to understand the proper dose and duration of supplementation with polyphenols-rich extracts in order to guide effective therapeutic interventions in diabetic patients.

  6. Study on Seabuckthorn Compound Juice Recipe%沙棘复合果蔬汁饮料配方研究

    Institute of Scientific and Technical Information of China (English)

    宋翔; 李悦; 魏文静; 康明艳

    2015-01-01

    以沙棘、西红柿、胡萝卜3种果蔬汁为原料生产复合果蔬饮料,通过正交试验筛选出该复合保健果蔬汁的最佳配比应为:沙棘汁20%、西红柿汁10%、胡萝卜汁15%、蔗糖10%。酸味剂选择柠檬酸,用量为0.15%;护色剂选用茶多酚,用量为0.12%;稳定剂选用CMC-Na和海藻酸钠组成复合稳定剂,海藻酸钠用量0.15%、CMC-Na用量1.2%。%Seabuckthorn, tomatoes, carrots 3 kinds of juice as raw material to produce composite fruit and vegetable juice, orthogonal experiment was used to select optimum ratio of the composite health care fruit and vegetable juice should be:sea buckthorn juice 20%, tomato juice 10%, sugar 10%, Fresh Carrot Juice 15%. Acid and citric acid, dosage of 0.15%;color protecting agent selection of tea polyphenols, dosage was 0.1%;the stabilizer CMC-Na and sodium alginate composite stabilizer , sodium alginate was 0.10%, the dosage of CMC-Na 15%.

  7. Addendum to aphids’ fauna, feeding on flowering plants in Krasnoyarsk krai

    Directory of Open Access Journals (Sweden)

    D. L. Grodnitsky

    2016-06-01

    Full Text Available The paper reports on new and previously known data on insufficiently studied fauna of aphids living on flowering plants in Krasnoyarsk region, the main part of a vast geographical province named Central or Middle Siberia, depending on terminology used by authors of literature sources. From physical geography viewpoint, the surveyed territory belongs to the Yenisey river basin. Aphids are an ecologically and morphologically diverse group of insects included in 11 families of the suborder Aphidinea in insect fauna of Russian Federation and 25 families in the world’s fauna. Geographical location, dates of collection of aphid colonies are presented with identification of species-specific feeding preferences. First found in Siberia were Symydobius nanae, Euceraphis betulae, Cavariella cicutae, C. konoi, Uroleucon (Dactynotus aeneum, U. giganteum, U. nigrocampalulae, U. trachelii, Aphis agrimoniae, A. coronillae, A. mongolica, A. neothalictri, A. pruni, A. pseudocomosa, A. pulsatillae, A. sambuci, A. (Debilisiphon umbelliferarum. Collected for the first time from willow were Betacallis comes (normally feeds on birch and alder, B. quadrituberculata (common on birch, colonies of Metopeurum fuscoviride were first found on Achillea millefolium. Aphis craccivora was previously collected from plants of different families, but was never seen on Cruciferae (Capsella bursa­pastoris. Aphis frangulae was concidered to feed exclusively on buckthorn, however, it was found on Chamaenerion angustifolium. Judging on diversity of host species, some aphids may be more important for agriculture than it was previously thought. A general review of Krasnoyarsk Krai aphid fauna is suggested for the first time ever.

  8. Natural herbs used in cosmetics and its efficacy%用于化妆品中的常见天然中草药及其功效

    Institute of Scientific and Technical Information of China (English)

    李峰; 王涛

    2012-01-01

    介绍了黄芩、芦荟、沙棘以及何首乌等20多种可应用于美容护肤类及护发乌发类化妆品中的常见天然中草药及其功效,并结合中医药理论简单分析了皮肤老化原因,指出天然中草药中含有很多的活性成分,将这些功效成分合理利用,有针对性地应用于化妆品中,对植物资源的开发利用以及功能化妆品的研制具有重要意义。%This paper introduced several natural herbs along with their efficacy,which can be used commonly in beauty skin care and hair care,such as scutellaria baicalensis,aloe,sea-buckthorn.It simply analyzed skin ageing problem combined with Chinese traditional medicine theory,pointed out the major active ingredients in these natural plants.It would have great significance in plant resources utilization and functional cosmetic development,if these functional compositions from natural herbal plants were used reasonably.

  9. Isorhamnetin ameliorates LPS-induced inflammatory response through downregulation of NF-κB signaling.

    Science.gov (United States)

    Li, Yang; Chi, Gefu; Shen, Bingyu; Tian, Ye; Feng, Haihua

    2016-08-01

    Isorhamnetin, a flavonoid mainly found in Hippophae fhamnoides L. fruit, has been known for its antioxidant activity and its ability to regulate immune response. In this study, we investigated whether isorhamnetin exerts potent antiinflammatory effects in RAW264.7 cell and mouse model stimulated by LPS. The cytokine (TNF-α, IL-1β, and IL-6) levels were determined. In the mouse model of acute lung injury, the phosphorylation of NF-κB proteins was analyzed and inhibitor of NF-κB signaling (PDTC) was used on mice. Our results showed that isorhamnetin markedly decreased TNF-α, IL-1β, and IL-6 concentrations and suppressed the activation of NF-κB signaling. Meanwhile, isorhamnetin reduced the amount of inflammatory cells, the lung wet-to-dry weight ratio, protein leakage, and myeloperoxidase activity. Interference with specific inhibitor revealed that isorhamnetin-mediated suppression of cytokines and protein was via NF-κB signaling. So, it suggests that isorhamnetin might be a potential therapeutic agent for preventing inflammatory diseases.

  10. Feeding habits and economic threshold of Locusta migratoria tibetensis%西藏飞蝗食性及其防治指标

    Institute of Scientific and Technical Information of China (English)

    杨群芳; 廖志昌; 李庆; 杨刚; 封传红; 蒋春先

    2008-01-01

    采用室外笼罩法测定西藏飞蝗Locusta migratoria tibetensis Chen的食性、食量及其对青稞和牧草造成的产量损失,并初步制定其防治指标.结果表明:西藏飞蝗1~2龄蝗蝻喜食牧草,3龄后喜食青稞和冬小麦;其在草地(亚高山草甸)、青稞和冬小麦3种植被上均能获得必需的营养并完成发育,而在沙棘Hippophae rhamnoides和白菜地则不能完成整个生活史;西藏飞蝗的取食量在1~3龄时很小,5龄和成虫期为暴食期;西藏飞蝗取食青稞和牧草的防治指标分别为3.01和4.44头/m2.

  11. A study of the development of bio-energy resources and the status of eco-society in China

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xia; Huang, Yongmei; Gong, Jirui [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Zhang, Xinshi [State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875 (China); College of Resources Science and Technology, Beijing Normal University, Beijing 100875 (China); Institute of Botany, CAS, Beijing 100093 (China)

    2010-11-15

    Industrialization of bio-energy relies on the supply of resources on a large scale. The theoretical biomass resources could reach 2.61-3.51 billion tce (tons of coal equivalent)/a in China, while the available feedstock is about 440-640 million tce/a, however, among this only 1.5-2.5% has been transferred into energy at present. Marginal land utilization has great prospects of supplying bio-energy resources in China, with co-benefits, such as carbon sequestration, water/soil conservation, and wind erosion protection. There is a large area of marginal land in China, especially in northern China, including about 263 million ha of desertification land, 173 million ha of sand-land, and 17 million ha of salinizatin land. The plant species suitable to be grown in marginal lands, including some species in Salix, Hippophae, Tamarix, Caragana, and Prunus is also abundant Biomass feedstock in marginal lands would be 100 million tce/a in 2020, and 200 million tce/a in 2050. As a result, a win-win situation of eco-society and bio-energy development could be realized, with an expected 4-5% reduction of total CO{sub 2} emission in China in 2020-2050. Although much progress has been made in the field of bio-energy research in China, yet significant efforts should be taken in the future to fulfill large-scale industrialization of bio-energy. (author)

  12. Ethnomedicine in Himalaya: a case study from Dolpa, Humla, Jumla and Mustang districts of Nepal

    Directory of Open Access Journals (Sweden)

    Kshhetri Hari B

    2006-06-01

    Full Text Available Abstract Traditional plant use in Nepal has been documented for millennia. The importance of plants as medicine has not diminished in any way in recent times, and traditional medicines are still the most important health care source for the vast majority of the population. This paper examines the ethnobotany and traditional use of plants extracted from the vulnerable alpine zone in the Dolpa, Humla, Jumla and Mustang districts of Nepal. The results of this ethnobotanical study indicate that a very large number of plant species is used as traditional medicines. There were 107, 59, 44 and 166 species of ethnomedicinal importance in surveyed areas of Dolpa, Humla, Jumla and Mustang district respectively. Of these, 84 common species, used at least in two districts, were selected to enumerate their ethnomedicinal properties. The 84 species belonged to 75 genera and 39 families. The commonest species in this pharmacopoeia were: Allium wallichii, Cordyceps sinensis, Dactylorhiza hatagirea, and Rheum australe. A total of 21 species were most common in three districts and 59 in two districts. The genera Aconitum, Allium, Arisaema, Berberis, Corydalis, Gentiana, Hippophae, Juniperus and Rhododendron each possessed two species with ethnomedicinal use. Labiatae was the most medicinally important family with five species used, followed by Araceae, Compositae, Liliaceae, Polygonaceae, Ranunculaceae, Scrophulariaceae and Umbelliferae, each contributing four species.

  13. [Species-associated differences in foliage-root coupling soil-reinforcement and anti-erosion].

    Science.gov (United States)

    Liu, Fu-quan; Liu, Jing; Nao, Min; Yao, Xi-jun; Zheng, Yong-gang; Li, You-fang; Su, Yu; Wang, Chen-jia

    2015-02-01

    This paper took four kinds of common soil and water conservation plants of the study area, Caragana microphylla, Salix psammophila, Artemisia sphaerocephala and Hippophae rhamnides at ages of 4 as the research object. Thirteen indicators, i.e., single shrub to reduce wind velocity ration, shelterbelt reducing wind velocity ration, community reducing wind velocity ration, taproot tensile strength, representative root constitutive properties, representative root elasticity modulus, lateral root branch tensile strength, accumulative surface area, root-soil interface sheer strength, interface friction coefficient, accumulative root length, root-soil composite cohesive, root-soil composite equivalent friction angle, reflecting the characteristics of windbreak and roots, were chose to evaluate the differences of foliage-root coupling soil-reinforcement and anti-erosion among four kinds of plants by analytic hierarchy process (AHP) under the condition of spring gale and summer rainstorm, respectively. The results showed the anti-erosion index of foliage-root coupling was in the sequence of S. psammophila (0.841) > C. microphylla (0.454) > A. sphaerocephala (-0.466) > H. rhamnides (-0.829) in spring gale, and C. microphylla (0.841) > S. psammophila (0. 474) > A. sphaerocephala (-0.470) > H. rhamnides (-0.844) in summer rainstorm. S. psammophila could be regarded as one of the most important windbreak and anti-erosion species, while C. microphylla could be the most valuable soil and water conservation plant for the study area.

  14. Airborne pollen assemblages and weather regime in the central-eastern Loess Plateau, China

    Science.gov (United States)

    Li, Yuecong; Ge, Yawen; Xu, Qinghai; Bunting, Jane M.; Lv, Suqing; Wang, Junting; Li, Zetao

    2015-04-01

    This paper presents the results of pollen trapping studies designed to quantify the pollen assemblages carried in the winds of the Loess Plateau in Luochuan and Hunyuan. The one-year-collection samples analysis results show that pollen assemblages can be more sensitive to the change of climate than the vegetation composition, because of the change of pollen production. The analysis results of pollen traps in different weather regimes indicate that the pollen influx coming from dust weather contribute more to the total pollen influx than that coming from non-dust weather. The wind speed is the most important influenced factor to pollen assemblages, then the mean temperature and the mean relative humidity, the wind direction also contributes some. Strong wind coming from dust direction can make the percent and influx of Artemisia and Chenopodiaceae increase obviously with averagely higher than over 2.7 times in dust weather than in non-dust samples. The influences of wind speed and wind direction are not serious to some arboreal pollen such as Rosaceae, Quercus, Betula, Pinus and Ostryopsis, which are mainly influenced by temperature or the relative humidity such as Salix, Hippophae, Carpinus, Brassicaceae, Cupressaceae, Fabaceae.

  15. [Difference of anti-fracture mechanical characteristics between lateral-root branches and adjacent upper straight roots of four plant species in vigorous growth period].

    Science.gov (United States)

    Liu, Peng-fei; Liu, Jing; Zhu, Hong-hui; Zhang, Xin; Zhang, Ge; Li, You-fang; Su, Yu; Wang, Chen-jia

    2016-01-01

    Taking four plant species, Caragana korshinskii, Salix psammophila, Hippophae rhamnides and Artemisia sphaerocephala, which were 3-4 years old and in vigorous growth period, as test materials, the anti-fracture forces of lateral-root branches and adjacent upper straight roots were measured with the self-made fixture and the instrument of TY 8000. The lateral-root branches were vital and the diameters were 1-4 mm. The results showed that the anti-fracture force and anti-fracture strength of lateral-root branches were lesser than those of the adjacent upper straight roots even though the average diameter of lateral-root branches was greater. The ratios of anti-fracture strength of lateral-root branches to the adjacent upper straight roots were 71.5% for C. korshinskii, 62.9% for S. psammophila, 45.4% for H. rhamnides and 35.4% for A. sphaerocephala. For the four plants, the anti-fracture force positively correlated with the diameter in a power function, while the anti-fracture strength negatively correlated with diameter in a power function. The anti-fracture strengths of lateral-root branches and adjacent upper straight roots for the four species followed the sequence of C. korshinskii (33.66 and 47.06 MPa) > S. psammophila (17.31 and 27.54 MPa) > H. rhamnides (3.97 and 8.75 MPa) > A. sphaerphala (2.18 and 6.15 MPa).

  16. A Study on Distribution Patterns of Soil Microorganisms in Refuse Dumps on Heidaigou Open Cut Coal Mine%黑岱沟煤矿排土场土壤微生物分布特征研究

    Institute of Scientific and Technical Information of China (English)

    常英; 包俊江

    2012-01-01

    By use of dilution plate counting method,a study has been made on the quantities, distribution patterns and compositions of soil microorganisms in different restored vegetation of refuse dumps on Heidaigou Open Cut Coal Mine. Results show that; (1) the total quantity of soil microorganisms in the resumed field is lower than that in the original undisturbed soil, and the maximum quantity reaches only 52.9% of that in the o-riginal soil; (2) within the depth of 0 ~30 cm,the total quantity of microorganisms in original soil decreases by order of 10 - 20 cm > 0 - 10 cm > 20 ~ 30 cm, while, with the increase of vertical depth of the resumed field, the total quantity decreases accordingly; (3) among different soil microorganism groups, the numbers of microorganisms all display the order that the number of bacteria > the number of actinomycetes > the number of fungi; (4) in resumed fields with different vegetation, the change pattern of the number of bacteria in the soil is generally similar to that of the total number of microorganisms, namely:that in original soil > in sea -buckthorn + armeniaca ansu + elaeagnus resumed field > in fruit tree resumed field > in torch tree resumed field > in sea - buckthorn resumed field, while the change pattern of the number of actinomycetes and fungi in different depth of different vegetation doesnt show the same trend; (5) the distribution of soil microorganism quantity in different vertical depth is associated with types of plant community.%利用稀释平板法对黑岱沟煤矿排土场不同恢复植被下的土壤微生物群落的数量、组成和分布进行了研究.结果表明:(Ⅰ)各恢复地土壤中微生物总数均低于未破坏土壤(原土),最高仅为原土的52.9%.(2)在0~30 cm范围内,原土微生物总数变化为:10~20 cm>0~10 cm >20 ~30cm,而各恢复地中微生物总数在0~30 cm范围内,随土壤垂直深度的加深而降低.(3)各样地土壤微生物数均

  17. 醋柳黄酮临床研究进展

    Institute of Scientific and Technical Information of China (English)

    陈和生

    1999-01-01

    @@ 心达康片主要成分为醋柳总黄酮.醋柳又名沙棘,是一种野生植物,属胡颓子科,学名Hippophae rhamniodes L .系蒙古族、藏族习用药材.中华人民共和国药典1977年版后各版均有收载[1].华西医科大学70年代后期从醋柳果实中提取出黄酮类化合物.经鉴定证明醋柳果渣中至少含有七种黄酮化合物,对含量较高的两种黄酮,采用化学方法,紫外吸收光谱,红外吸收光谱,质谱及核磁共振谱鉴定,证明为异鼠李素(Isorhamnetin)和槲皮素(Quercetin)[2].醋柳黄酮包括异鼠李素和槲皮素及其苷.其苷有:异鼠李素-3-0-葡萄糖苷、异鼠李素-3-0-芸香糖苷、异鼠李素-3-葡萄糖-7-鼠李糖苷,异鼠李素-3-葡萄糖[1.2]葡萄糖苷,槲皮素-3-葡萄糖苷、槲皮素-3-芸香糖苷等.其中含量较高的为异鼠李素-3-葡萄糖苷[3].

  18. Evaluation of phytochemical constituents and antioxidant activity of selected actinorhizal fruits growing in the forests of Northeast India

    Indian Academy of Sciences (India)

    Arvind K Goyal; Tanmayee Mishra; Malay Bhattacharya; Pallab Kar; Arnab Sen

    2013-11-01

    Hippophae salicifolia, Elaeagnus pyriformis, Myrica esculenta and M. nagi are actinorhizal plants growing in the sacred forests of Northeast India with multipurpose uses. The present investigation was undertaken to determine the phenol, flavonoid and flavonol contents of the fresh fruit juice of these plant species including the antioxidant potential by means of DPPH, H2O2 and NO scavenging activity and FRP. The total phenolic, flavonoid and flavonol contents of fruit juice ranged from 321.68±0.06 to 76.67±0.01 mg/g GAE, 272.92±0.07 to 20.12±0.02 mg/g QE and 258.92±0.08 to 18.72±0.02 mg/g QE, respectively. At 2.0 mg/mL concentration, DPPH scavenging activity was found to be the highest in M. esculenta (89.62%) and the lowest in E. pyriformis (17.58%). The reducing power activity was found significantly higher in H. salicifolia juice, which increased with increase in concentration. The H2O2 scavenging activity of H. salicifolia juice was found to be as high as 98.78%, while Elaeagnus juice was found to be less effective with just 48.90%. Juice of H. salicifolia showed the greatest NO scavenging effect of 75.24% as compared to juice of E. pyriformis, where only 37.54% scavenging was observed at the same concentration. Taking into account all the experimental data, it can be said that the fruits of H. salicifolia and both M. nagi and M. esculenta have good antioxidant activity compared to fruits of E. pyriformis.

  19. SORPTION OF CO2-EXTRACTS OF SPICES TO THE TOMATO PASTE

    Directory of Open Access Journals (Sweden)

    A. M. Gadzhieva

    2014-01-01

    Full Text Available The possibility of the production of products without the preservatives, the fillers, the chemical, undesirable aromatic substances and other additives is one of the prime advantages of technologies, based on the use of СO2- extraction. There is great theoretical and practical interest in the study of the absorption of СO2- extracts on the tomato carriers with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. There are examined the principles of the optimization of the process of absorbing the СO2- extracts of anise, ginger and amaranth on the tomato paste with the production of ketchup. The miscella of the extracts of spices is brought to the tomato paste. This was reached by the selection of СO2- miscella from the extractor under the pressure to 6 MPa. Then solvent due to a pressure decrease in the apparatus to the atmospheric instantly was moved away from the obtained material. The degrees of the absorption of the СO2- extracts of anise, ginger, amaranth and sea buckthorn are determined. Calculations were performed through the program of that composed in medium of the mathematical packet “Of mathCAD 14”. As a result conducted investigations are obtained the equations of regression for absorbing of anise, amaranth and ginger on the tomato paste depending on the concentration of individual extracts in the miscella. The possibilities of the method of complete factor experiment are shown. In the utilized method of the mathematical planning of experiment adapt the dimensionless coded composition factors x1 and x2. In the experiment is realized plan 23. Was used the central composition plan, which corresponds to the requirement of rotatable, i.e., the plan, which makes it possible to obtain the model, capable of predicting the value of the parameter of optimization with the

  20. Impact of subjacent rocks at the water and air regime of the depleted peat deposits

    Science.gov (United States)

    Rakovich, V. A.

    2009-04-01

    At the depleted peat deposits (after peat extraction), where the residual layer of peat with the thickness of about 0,5 meters is laid at the well water permeable rocks, vegetation typical for dry conditions is developed in case of good drainage conditions; birch trees, willow, alder-trees and buckthorn prevail in this vegetation. Water and air regime is characterized here by good aeration with prevailing of oxidative processes. If water regime is regulated, these depleted peat areas are suitable for agricultural and forest lands; however, necessity of transformation of these depleted lands into forest and agricultural lands must be ecologically and economically justified. If the residual layer of peat with the thickness of 0,05-0,3 m is based at the sapropel or peat sapropel, contrast amphibiotic water and air regime with strong fluctuation of oxidative and restoration process depending on the weather conditions is formed; this regime is formed without artificial increase of the ground waters level. This does not allow bog vegetation or vegetation typical for dry conditions to develop. Thus, within 20 and more years after completion of peat extraction, such areas are not covered by vegetation in spite of favorable agro-chemical qualities of peat layer and favorable for vegetation chemical composition of soil and ground waters. Depleted peat deposits, that are based at the sapropel, are not suitable for agricultural use, because agricultural vegetation requires stable water and air regime with good aeration and oxidative and restoration potential within 400-750 mV. Contrast amphibiotic water and air regime of the depleted peat deposits that are based at sapropel excludes possibility to use them as agricultural lands. Because of this reason, areas with residual peat layer that are based at sapropel are not suitable for forest planting. Due to periodic increase of ground waters level, rot systems of the plants can not penetrate into the required depth, and mechanical

  1. Land Desertification and it’s Control in Gonghe Basin of Qinghai Plateau, China

    Science.gov (United States)

    Zhang, D.; Gao, S.; Lu, R.

    2009-12-01

    seeding > Caragana direct seeding, Tamarix cutting and seedling > Salix cheilophila deep planting, Sea-buckthorn seedling > Tamarix deep planting > Tamarix seedling > Poplar deep planting > Salix cheilophila seedling > Poplar seedling. It has resolved the key problem of control sand flow speed and low efficiency, sand burying and wind erosion and low conservation rate for forestation in the sandy area.

  2. Host plant shifts and transitions into new adaptive zones in leafhoppers: the example of Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) of Russia and adjacent countries.

    Science.gov (United States)

    Tishechkin, Dmitri Yu

    2016-06-08

    The modes of diversification of Palaearctic Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) are reconstructed based on data on their host plants and distribution in Russia and the adjacent territories. Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) is originally an Oriental group, which penetrated into the Palaearctic from Southeast Asia. The genus Pediopsoides and species of the genus Macropsis that feed on East Asian oaks have not dispersed beyond broadleaf forests of the Eastern Palaearctic. Apparently, Pediopsis and elm-feeding species of Macropsis initially dispersed throughout the entire broadleaf forest zone. Division of this zone into two widely separated parts in temperate areas of Europe and East Asia (nemoral disjunction), produced closely related vicariant pairs of sister species. The genus Oncopsis and species of Macropsis feeding on Salicaceae dispersed throughout the entire Palaearctic following their host plants. Both lineages penetrated into riparian forests of the foothills and midlands of Central Asia, where they produced endemic species. The Central Asian Macropsis lineage shifted from Salicaceae to trees and shrubs of unrelated families (wild roses, barberry, oleaster, and sea-buckthorn) growing in the same biotopes. Subsequent diversification on those plants produced several separate host-associated species-groups, some of which penetrated following their hosts from riparian forests into arid habitats. One such lineage apparently shifted from shrubs to wormwood species (Artemisia spp.) and thus gave rise to the genus Macropsidius. This genus underwent adaptive radiation on wormwood species in the plains of South Kazakhstan and Central Asia; advancing westward, it formed secondary centres of diversity in Transcaucasia and the Mediterranean. Finally, some lineage of Macropsidius (or its sister-group) switched from feeding on Artemisia to polyphagy, yielding the ancestral form of the genus Hephathus. In general, the evolution of

  3. COMPOSIÇÃO E ESTRUTURA DO COMPONENTE ARBÓREO DE MATA COM ACURI NO PANTANAL MATOGROSSENSE, BRASIL

    Directory of Open Access Journals (Sweden)

    Raquel Rejane Bonato Negrelle

    2016-01-01

    Full Text Available Aiming to contribute to the better understanding of this kind of Pantanal forest, the results from a floristic- structural survey performed in a 1ha plot commonly classified as “Acuri Forest”, located at RPPN SESC Pantanal (Municipality of Barão de Melgaço - MT, Brazil - 16º45’S; 56º15’W are presented. The floristic data were obtained from systematic collection of botanical material on the studied area and surroundings. The structural data were obtained from a one ha plot, divided into 100 contiguous sub-plots (10m x 10m. Inside these sub-plots, all arboreal individuals with diameter at breast high equal or greater than 5.0 cm, including palms, were identified, measured and mapped, using a coordinate system. 1224 individual ha -1 were sampled, representing 44 species, 43genus and 27 families; diversity index H’= 1.613 (var. 0.003514. The highest diversity families were Fabaceae (9 spp., Sapindaceae e Anacardiaceae (3 spp. each, Bignoniaceae, Malvaceae, Moraceae e Rubiaceae (2 spp. each. Among the sampled species, 39 % were evergreen; 32 % semideciduous and 29% deciduous. Considering the tree individuals total (n = 440, 51 % were decíduos; 23% semideciduous and 26% evergreen . Attalea phalerata was the species with the highest structural value (VI= 121.62, due to its high density (64% of the total sampled individuals, frequency (100% e and very high basal area (74.10 m 2 ha -1 . On second level of importance were Anadenanthera colubrina, Triplaris americana and Phyllostylon rhamnoide (VI= 35.41; 28.51; 18.5 respectively. The studied area was identified as very peculiar comparatively with other Seasonal Forest areas, especially because the Attalea phalerata massive density and absence of Myrtaceae representatives. The high representation of pioneer species in association with high density of individuals within this successional category reinforces that Mata com Acuri represents an early successional stage or a transitional stage

  4. Climate-landform effects on lateglacial vegetation pattern in northeastern Tuchola Pinewoods (northern Poland): multiproxy evidence from the Lake Czechowskie catchment, northern Poland.

    Science.gov (United States)

    Noryśkiewicz, Agnieszka M.; Kordowski, Jarosław; Tyszkowski, Sebastian; Kramkowski, Mateusz; Zawiska, Izabela; Rzodkiewicz, Monika; Mirosław-Grabowska, Joanna; Ott, Florian; Słowiński, Michał; Obremska, Milena; Błaszkiewicz, Mirosław; Brauer, Achim

    2016-04-01

    The study area is located in northern Poland in the northeastern part of Tuchola Pinewoods in a young glacially formed and diversified landscape. It comprises the entire lake catchment of Lake Czechowskie (19.76 km2), which comprises a second lake upstream as well as a palaeolake (Trzechowskie) located between the two present-day lakes. Biogenic sediments from eight cores were studied by multiproxy analyses to reconstruct the environmental changes and climate signals during the last Late Glacial and early Holocene. The cores were collected along a W-E transect from Głęboczek Lake to the Czechowskie Lake and were located in different topographic positions (deepest and shallow part of the lake, old lake-bed plains and paleolakes) with a maximum distance of 2.2 km. Detailed and high resolution analyses (pollen, diatoms, cladocera, stable isotopes, geochemistry, varve chronology and radiocarbon dating) to identify the main stages in the development of the natural environment were made. Palynological data indicate melting of the buried ice blocks and the following the onset of biogenic lacustrine sedimentation. The general pattern of vegetation changes in all profiles is similar and includes Late Glacial steppe-tundra plant communities at the onset of organic lake sedimentation. The palynological record of the most profiles shows a high participation of seabuckthorn (Hippophae) in the initial stadium of vegetation history. The lack of this succession in the most western core (Głęboczek Lake) indicates a later period of melt-out processes of the buried dead-ice blocks in the Głęboczek Lake basin. The thickness and type of the accumulated sediments differ significantly during the Bolling-Alerod complex and Younger Dryas Period between our sites. These differences are also reflected in variations of plant species among the different sites. The comparison of different profiles within one catchment allows us to distinguish site specific local responses to climate

  5. Effects of invasive plant species on pollinator service and reproduction in native plants at Acadia National Park

    Science.gov (United States)

    Stubbs, C.J.; Drummond, F.; Ginsberg, H.

    2007-01-01

    Invasive plant species can have profound negative effects on natural communities by competively excluding native species. Berberis thunbergii (Japanese barberry), Frangula alnus (glossy or alder buckthorn) and Lythrum salicaria (purple loosestrife) are invasive species known to reduce native plant diversity and are thus of great concern to Acadia National Park. Pollinators visit them for nectar and pollen. The effects of invasive plant species on pollinator behavior were investigated by comparing pollinator visitation to co-flowering native and invasive species with visitation to native species growing alone. The effect of invasives on pollination of native plants was studied by comparing fruit set in patches of the native species growing near invasives with patches far from invasive species in Acadia National Park. The coflowering pairs were as follows: in the spring native Vaccinium angustifolium (lowbush blueberry) was paired with B. thunbergii; in early summer native Viburnum nudum (wild raisin) was paired with F. alnus ; in late summer native Spiraea alba (meadowsweet) was paired with L. salicaria. We investigated whether these invasives competed with native plants for pollinators in Acadia and thus negatively affected native plant reproduction. Our objectives were to determine: 1) the influence, if any, of each invasive on pollinator visitation to a co-flowering native species, 2) factors that might affect visitation, 3) invasive pollen transfer to native plants, and 4) whether invasives influence native plant reproduction (fruit set). Our findings indicate that at times the number of flower visitors to natives was lower or the species composition of visitors different when invasives were present, that invasives sometimes attracted more pollinators, that generally the invasives were more rewarding as far as nectar and pollen availability for pollinators, and that generally native plant fruit set and seed set was not significantly lowered in the presence of

  6. 西北黄土高原区路域乡土植物资源筛选研究%Selection of Indigenous Plants in Roads in Northwestern Loess Plateau Area

    Institute of Scientific and Technical Information of China (English)

    张铁军; 王春玲

    2012-01-01

    , Atriplex canescens, Amygdalus triloba, Hippophae rharnnoides Linn., Arnorpha fruticosa L., and Syzygium aromaticum could be planted. In addition, Gleditsia sinensis Lain, Robinia pseudoacacia L, Elaeagnus angustifolia L., Rhus typhina, Salix babylonica, Salixmatsudana and Fraxinus chinensis, tall and strong, are suitable to be planted as border trees, presenting a good vision. Frutices, limited by height, are usually with more branches, which will provide a good landscape if grown with tall plants. Prunus dacidiana and Ulmus pumila L., prone to be damaged by insects, should be planted with other species to stop the infestation in space. [Conclusion] The research laid foundation for selection of indigenous plants in roads, providing references for resource shortage of indigenous plant for bioengineedng.

  7. 侧柏与其他树种枯落叶混合分解对养分释放的影响%IMPACTS OF DECOMPOSITION OF MIXTURE OF LEAF LITTERS FROM PLATYCLADUS ORIENTALIS AND OTHER TREES ON NUTRIENT RELEASE

    Institute of Scientific and Technical Information of China (English)

    张晓曦; 刘增文; 祝振华; 杜良贞

    2013-01-01

    constant temperature and humidity for 345 days on end.The litters were analyzed for quality and nutrient contents before and after the incubation.Results show that 1) among the nutrients released during decompostion of the leaf litters,K was the most active and easy to get released,while P was generally dull and difficult to get released,and C and N was moderate and often synchronous in releasing,and the recycle period of these nutrients was 1 ~ 2 a; 2) leaf litter of Ulmus pumila in the mixture acted positively,while that of Amorpha fruticosa,Betula platyphylla and Quercus liaotungensis negatively on C release ; leaf litter of Populus simonii did positively,while that of B.platyphylla,Q.liaotungensis and A.fruticosa negatively on N release; litters of P.simonii and U.pumila did positively,while litters from all the other trees negatively on P release; and litter of U.pumila did positively,while that of A.fruticosa did negatively on K release;3) the Principal Component Analysis of comprehensive effects of decomposition of various mixtures of leaf litters on releasing of C,N,P and K revealed that leaf letter of U.Pumila and P.simonii in the mixture was the most significant in promoting nutrient release,and litters from Caragana microphylla and Q.liaotungensis followed,Litter from A.fruticosa,followed by litter from B.platyphylla,H.rhamnoides and R.Pseudoacacia,showed obvious inhibitive effects on nutrient release.

  8. 黑河下游荒漠河岸林植物水分利用关系研究%Relationship among water use of different plants in Heihe River riparian forests

    Institute of Scientific and Technical Information of China (English)

    陈小丽; 陈亚宁; 陈亚鹏

    2014-01-01

    This paper analyzed the sources of water for desert riparian forests and identified the sources of water for different plant species. In order to explore the relationships among the plant species, the spatial differences in the sources of water for different forest plants were analyzed. The study provided the basis for locating ecological desert river forest oases with mixed plant species and for protecting inter-species interactions by monitoring aggressive water use and competition. A multi-isotope mass balance analysis was used to explore the potential contributions of different sources of water to plant. The xylem water and potential water resources including soil water and groundwater, ofPopulus euphratica(seedlings),Tamarix karelinii,Sophora alopecuroides,Hip-pophae rhamnoides subsp. sinensis,Karelinia caspia andPeganum nigellastrum were analyzed by using stable oxygen isotope composition (δD,δ18O) measurements and analyses. The results showed differentδD,δ18O values in xylem water of different types of plants. This suggested that different plants absorbed water from different depths. The analysis showed that the main soil depth of absorbed water was 0-20 cm forP. euphratica seedlings, 200-300 cm forT. karelinii, 0-20 cm for H. rhamnoidessubsp.sinensis, 50-100 cm forK. caspia, 0-20 cm forP. nigellastrum and 0-5 cm forS. alopecuroides. The contribution rates of different potential water resources were different to different plant species. Herbaceous plants mainly used shallow soil water. While shrubs with different plant species had different sources of water,P. euphratica also mainly used the shallow soil water. Based on the results of the study, competitions were noted amongP. euphratica seedlings,H. rhamnoidessubsp.sinensisandP. nigellastrum in the Heihe River desert riparian forests in terms of water use.P. euphratica andT. karelinii enhanced the water use of each other in the study area. Evaporation of soil moisture was the cause of enriched stable

  9. 刺槐与其他树种枯落叶混合分解对微量元素释放的影响%Influences of mixed leaf litter decomposition of Robinia pseudoacacia with other trees on microelements release

    Institute of Scientific and Technical Information of China (English)

    杜良贞; 刘增文; 祝振华; 李茜

    2012-01-01

    whole Loess Plateau, through collecting leaf litter of R. pseudoacacia and other 10 trees at the end of autumn,cutting the leaf to some fragments (a=l cm) or 1 cm needles,and gathering humus soil (0-20 cm) of no forested land as medium,the incubation of mixed leaf litter decomposition had been carried out for 345 d with the method of nylon mesh bag buried with soil in the same condition of tem- perature and moisture. Then,the microelements (Cu,Zn,Ni,Cd) contents before and after leaf litter de-composition were determinated with the Atomic Absorption Spectroscopy(AAS)method. [Result] The re- lease rates of Cu,Zn,Ni and Cd during unique leaf litter decomposition of Populus simonii ,Hippophae rh- amnoides ,Platycladus orientalis and Quercus liaotungensis are all the fastest and the turnover period is 1 year or a little more. Mixing litter decomposition of R. pseudoacacia with P. simonii,Ulmus pumila, H. rh- amnoides separately would produce promoted actions on release of the microelements, whereas mixed litter decomposition of R. pseudoacacia with Pinus sylvestris var. mongolica, P. orientalis, Betula platyphylla and Caragana microphylla separately would produce inhibitory actions on release of the 4 microelements. Mixed litter decomposition of R. pseudoacacia with Pinus tabulaeformis and Larix principis-rupprechtii separately would promote release of Cu, but inhibit releases of Zn, Ni and Cd. Mixed litter decomposition of R. pseudoacacia with Q. liaotungensis would promote releases of Zn and Cd,but inhibit releases of Cu and Ni. [Conclusion] Mixed forestation of R. pseudoacacia with other trees which promote release of microele- ments after mixed litter decomposition would be helpful for the microelement cycling and utilization in for- estland.