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Sample records for buckthorn hippophae rhamnoides

  1. Remedial Prospective of Hippophae rhamnoides Linn. (Sea Buckthorn).

    Science.gov (United States)

    Patel, Chirag A; Divakar, Kalyani; Santani, Devdas; Solanki, Himanshu K; Thakkar, Jalaram H

    2012-01-01

    Sea buckthorn (Hippophae rhamnoides L.) constitutes thorny nitrogen fixing deciduous shrub. Sea buckthorn(SBT) is primarily valued for its very rich vitamins A, B(1), B(12), C, E, K, and P; flavonoids, lycopene, carotenoids, and phytosterols. and therapeutically important since it is rich with potent antioxidants. Scientifically evaluated pharmacological actions of SBT are like inflammation inhibited by reduced permeability, loss of follicular aggregation of lymphocytes from the inflamed synovium and suppress lymphocyte proliferation. SBT-reduced recurrence of angina, ischemic electrocardiogram which might be due to decreased myocardial oxygen consumption and inhibition of platelet aggregation induced by collagen. SBT can kill both cancer cells of S180, P388, SGC7901 and lymphatic leukemia (L1200). The antiulcer activity may be related to reduce gastric empty time, inhibiting proteolytic activity and promoting wound reparation processes of mucosa. SBT exerts antihypertensive effect in part by blocking angiotensin-2 receptor on cell surface. SBT decreased the level of stress hormones and enhanced hypoxic tolerance in animals indicating its anti-stress, adaptogenic activity. A lot of research work is still needed to find cellular and molecular mechanisms of these activities and also yet to be explored for its activity in osteoporosis, hemorrhage, cataract, urinary stone, acne, psoriasis, polyneuritis, cheilosis, glossities, baldness, anti-obesity, gout, and chronic prostitis. PMID:22530142

  2. A new agent for flap survival – Hippophae rhamnoides L. (sea buckthorn): An experimental study in rats

    OpenAIRE

    Emsen, Ilteris Murat

    2005-01-01

    Hippophae rhamnoides L. (sea buckthorn) is a member of the Elaeagnaceae family, and is a temperate bush native to Europe and Asia. The antioxidant activity of H rhamnoides L. has been shown in vitro cell culture and animal studies. Different fractions of H rhamnoides L. fruits inhibit 2,2-azobis-(2,4 dimethylvaleronitrile) and ascorbate iron-induced lipid peroxidations in vitro. H rhamnoides L., as well as vitamin E, decrease the malondialdehyde content in hyperlipidemic rabbit serum-cultured...

  3. PREPARATION OF PROTOPLASTS OF SEA BUCKTHORN (HIPPOPHAE RHAMNOIDES L.

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    M. V. Skaptsov

    2015-04-01

    Full Text Available The aim of research was to study the effect of a method of protoplast isolation on Hippophae rhamnoides cell viability. The main objectives were the release of protoplasts H. rhamnoides from callus tissue by a combination of mechanical and enzymatic effects, as well as evaluation of the general state of isolated cells after isolation and purification. The objects of studies were mesophyll cells and leaf mesophyll explants introduced in culture in vitro. It is assumed that considerable damage of cells in the process of mechanical-enzymatic treatment is not only a result of mechanical impact, but also an effect of the release of metabolic products (polyphenolic components of cells and their decay. Also protoplasts are dedifferentiated and simplify the process of differentiation in various breeding and biotechnological experiments. As a result of the work, an optimized method of H. rhamnoides protoplast isolation with decreased levels of cell damage is set up. It is revealed that important factors for high yield of viable protoplasts are: proper concentration of D-mannite as an osmoticum; proper concentration of cellulase R10 and macerozime R10; use of sodium tiosulfate as an antioxidante; proper time of incubation of leaf explants in medium with enzymes.

  4. Sea buckthorn (Hippophae rhamnoides L.) germplasm in South Warmia, Poland

    OpenAIRE

    Shalkevich, Maryna; Bieniek, Anna; Kawecki, Zdzisław

    2007-01-01

    One of the most important problems of sea buckthorn breeding program in Belarus is producing wilt-resistant cultivars that are suitable for machine harvesting. To introduce new parental forms into the breeding process, the examination of wild sea buckthorn plantations at the territory of South Warmia of Poland (Olsztyn, Poland) was carried out in 2006. First of all, the absence of visual wilt symptoms was taken into account. Also, the following criteria of suitability for machine harvesting w...

  5. Sea Buckthorn (Hippophaë rhamnoides ssp. rhamnoides) Berries in Nordic Environment: Compositional Response to Latitude and Weather Conditions.

    Science.gov (United States)

    Zheng, Jie; Kallio, Heikki; Yang, Baoru

    2016-06-22

    Flavonol glycosides (FGs) in sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) berries of varieties 'Tytti' and 'Terhi', cultivated in northern Finland (68°02' N) for six years and southern Finland (60°23' N) for seven years, were investigated and compared by HPLC-DAD-ESI-MS/MS. The average total content of 23 identified glycosides of isorhamnetin and quercetin was 103 ± 23 and 110 ± 21 mg/100 g fresh berries in 'Terhi' and 'Tytti', respectively. The total contents of FGs, flavonol diglycosides, and triglycosides in both varieties were higher in the north than in the south, whereas total flavonol monoglycoside content behaved vice versa (p < 0.05). Among the 89 weather variables studied, the sum of the daily mean temperatures that are 5 °C or higher from the start of growth season until the day of harvest was the most important variable which associated negatively with the accumulation of FGs in berries. Such influence was much stronger in berries from the north than from the south. PMID:27215398

  6. Protective Effect of Proanthocyanidins from Sea Buckthorn (Hippophae Rhamnoides L. Seed against Visible Light-Induced Retinal Degeneration in Vivo

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    Yong Wang

    2016-05-01

    Full Text Available Dietary proanthocyanidins (PACs as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L. seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6 and angiogenesis (VEGF levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.

  7. Effects of latitude and weather conditions on proanthocyanidins in berries of Finnish wild and cultivated sea buckthorn (Hippophaë rhamnoides L. ssp. rhamnoides).

    Science.gov (United States)

    Yang, Wei; Laaksonen, Oskar; Kallio, Heikki; Yang, Baoru

    2017-02-01

    Sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) of varieties 'Terhi' and 'Tytti' and one of wild origin were cultivated in southern and northern Finland, harvested during 2007-2013. Proanthocyanidins (PAs) were analyzed with HILIC UPLC-ESI-MS. The southern and northern samples were separated in the partial least squares discriminant analysis model (four factors, R(2) 0.75, Q(2) 0.70). The total PAs were more abundant in berries from the north (610-970mg/100gDW) than in those from the south (340-450mg/100gDW) (p<0.05). In northern Finland, the length of the growth season as well as the temperature sum and radiation sum of the growth season until harvest were negatively correlated with the total PAs in all the samples but positively with PA oligomers in 'Tytti' and 'Terhi'. In southern Finland no respective correlations were seen. 'Terhi' and 'Tytti' had different trends in the content of total PA and oligomers in overripe stages. PMID:27596396

  8. Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides, Apricot Pulp and Apricot Kernel (Prunus armeniaca

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    Elena Andreea Pop

    2015-11-01

    Full Text Available A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L. and apricot fruits (Prunus armeniaca L. are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits. The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC  method.  Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection. The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg than in apricot fruits (1.09 mg/kg while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg. Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.

  9. Metabolite profiling and expression analysis of flavonoid, vitamin C and tocopherol biosynthesis genes in the antioxidant-rich sea buckthorn (Hippophae rhamnoides L.).

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    Fatima, Tahira; Kesari, Vigya; Watt, Ian; Wishart, David; Todd, James F; Schroeder, William R; Paliyath, Gopinadhan; Krishna, Priti

    2015-10-01

    In this study, phenolic compounds were analyzed in developing berries of four Canadian grown sea buckthorn (Hippophae rhamnoides L.) cultivars ('RC-4', 'E6590', 'Chuyskaya' and 'Golden Rain') and in leaves of two of these cultivars. Among phenolic acids, p-coumaric acid was the highest in berries, while gallic acid was predominant in leaves. In the flavonoid class of compounds, myricetin/rutin, kaempferol, quercetin and isorhamnetin were detected in berries and leaves. Berries of the 'RC-4' cultivar had approximately ⩾ 2-fold higher levels of myricetin and quercetin at 17.5mg and 17.2 mg/100 g FW, respectively, than the other cultivars. The flavonoid content in leaves was considerably more than in berries with rutin and quercetin levels up to 135 mg and 105 mg/100 g FW, respectively. Orthologs of 15 flavonoid biosynthesis pathway genes were identified within the transcriptome of sea buckthorn mature seeds. Semi-quantitative RT-PCR analysis of these genes in developing berries indicated relatively higher expression of genes such as CHS, F3'H, DFR and LDOX in the 'RC-4' cultivar than in the 'Chuyskaya' cultivar. Vitamin C levels in ripened berries of the Canadian cultivars were on the high end of the concentration range reported for most other sea buckthorn cultivars. Orthologs of genes involved in vitamins C and E biosynthesis were also identified, expanding the genomic resources for this nutritionally important plant. PMID:26318327

  10. Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model.

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    Ito, Hiroshi; Asmussen, Sven; Traber, Daniel L; Cox, Robert A; Hawkins, Hal K; Connelly, Rhykka; Traber, Lillian D; Walker, Timothy W; Malgerud, Erik; Sakurai, Hiroyuki; Enkhbaatar, Perenlei

    2014-05-01

    To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, pseed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds. PMID:24007892

  11. The Impact of the Sea-buckthorn (Hippophae rhamnoides) Supplement in the Feed Ration on the Quality of Poultry Products

    OpenAIRE

    Pebriansyah, Akhir

    2014-01-01

    Seabuckthorn (Hippophae rhamnoides) (SB) is a shrub which belongs to the family Elaeagnaceae, it have been used in Asia, Europe, and North America for human consumption, cosmetics, and also animal feeding as a dried by-product after fruit processing for juices. This study was aimed to find any positive effect of the SB supplement in feed ration on the laying hens´ productivity and quality of eggs. Two experiments were conducted at the ITP (International Testing of Poultry) in Ústrašice, Czech...

  12. Effects of different origins and harvesting time on vitamin C, tocopherols, and tocotrienols in sea buckthorn (Hippophaë rhamnoides) berries.

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    Kallio, Heikki; Yang, Baoru; Peippo, Pekka

    2002-10-01

    Vitamin C, tocopherols, and tocotrienols in berries of wild and cultivated sea buckthorn (Hippophaë rhamnoides L.) of different origins and harvesting dates were determined with HPLC. Wild berries of subsp. sinensis, native to China, contained 5-10 times more vitamin C in the juice fraction than the berries of subsp. rhamnoides from Europe and subsp. mongolica from Russia (4-13 vs 0.02-2 g/L juice). Genetic background and berry-harvesting date were two primary factors determining the vitamin C content in the berries. Crossing different subspecies influenced the vitamin C content to some extent. For bushes cultivated in southwest Finland, the best berry-harvesting date for high vitamin C content was the end of August. The seeds of subsp. sinensis contained less tocopherols and tocotrienols (average 130 mg/kg) compared with seeds of subsp. rhamnoides (average 290 mg/kg) and mongolica (average 250 mg/kg). The fruit flesh of sinensis berries had contents of tocopherols and tocotrienols 2-3 times higher than those found in the other two subspecies (120 mg/kg vs 40 mg/kg in rhamnoides and 50 mg/kg in mongolica). The fresh whole berries of subsp. sinensis were clearly the best source of total tocopherols and tocotrienols. The total content of tocopherols and tocotrienols in the soft parts of the berries reached the maximum level around early- to mid-September, whereas the content in seeds continued to increase until the end of November. The excellent combination of the highest content of vitamin C and tocopherols and tocotrienols makes the berries of subsp. sinensis an optimal raw material for nutritional investigation as a candidate for functional foods with special antioxidative properties. PMID:12358492

  13. Flavonol glycosides in berries of two major subspecies of sea buckthorn (Hippophaë rhamnoides L.) and influence of growth sites.

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    Ma, Xueying; Laaksonen, Oskar; Zheng, Jie; Yang, Wei; Trépanier, Martin; Kallio, Heikki; Yang, Baoru

    2016-06-01

    Flavonol glycosides of wild sea buckthorn (Hippophaë rhamnoides ssp. sinensis) berries from China and cultivated berries (H. rhamnoides ssp. mongolica) from Finland and Canada were identified and quantified. Twenty-six flavonol glycosides were found with isorhamnetin and quercetin as the major aglycones. The contents of flavonol glycosides ranged 23-250 mg/100 g fresh berries and were significantly higher in the berries of ssp. sinensis than in those of ssp. mongolica. Among the cultivars of ssp. mongolica, the berries of 'Oranzhevaya' had the lowest (23 mg/100 g) content, and those of 'Prevoshodnaya' the highest content of flavonol glycosides (80 mg/100 g). Within the ssp. mongolica, the samples from Kittilä (Northern Finland) had higher levels of most flavonol glycosides than those from Turku (Southern Finland) and Québec. Among the ssp. sinensis berries of different growth sites, increasing trends were detected in the contents of most of the compounds as the altitude increased and as the latitude decreased. The wild berries (ssp. sinensis) from Sichuan had remarkably high contents and unique profiles of flavonol glycosides. PMID:26830578

  14. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD.

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    Zu, Yuangang; Li, Chunying; Fu, Yujie; Zhao, Chunjian

    2006-06-01

    A rapid and specific reversed-phase high performance liquid chromatography (RP-HPLC) method with diode array detection (DAD) at room temperature was used and validated for the simultaneous determination of five flavonoids (catechin, CA; rutin, RU; quercetin, QU; kaempferol, KA; isorhamnetin, IS) in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves. The sample pretreatment process involved ultrasonic extraction with 85% ethanol under the frequency of 80 kHz, at a temperature of 45 degrees C for 30 min and with the ratio of liquor to material of 15 mL g-1, followed by separation on HIQ SIL C18V column with methanol-acetonitrile-water (40:15:45, v/v/v) containing 1.0% acetic acid as a mobile phase. The extract was detected by DAD at the wavelength of 279 nm for CA, 257 nm for RU, 368 nm for QU, KA and IS. Calibration curves were found to be linear with the ranges of 0.011-0.520 mg ml-1 (CA), 0.007-0.500 mg ml-1 (RU), 0.019-0.280 mg ml-1 (QU), 0.010-0.440 mg ml-1 (KA) and 0.008-0.400 mg ml-1 (IS). The correlation coefficients of linear regression analysis and detection limits were between 0.9963-0.9999 and 0.00079-0.00290 mg ml-1. The contents of CA, RU, QU, KA and IS in sea buckthorn leaves were successfully determined with 3.8, 5.2, 7.3, 10.9 and 11.9 min with satisfactory reproducibility and recovery. Recoveries of the five flavonoids were between 97.27 and 99.98%. The method was applied to the determination of flavonoids in sea buckthorn leaves and was found to be simple, rapid and efficient. PMID:16520013

  15. Untargeted Metabolomics for Sea Buckthorn (Hippophae rhamnoides ssp. carpatica Berries and Leaves: Fourier Transform Infrared Spectroscopy as a Rapid Approach for Evaluation and Discrimination

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    Raluca M. POP

    2014-12-01

    Full Text Available Untargeted metabolomics coupled with chemometric analysis was applied to evaluate and discriminate six Romanian sea buckthorn (Hippophae rhamnoides L. berries and leaves. Total carotenoids and total phenolics were determined quantitatively by UV-Vis spectrometry. The qualitative evaluation and discrimination was obtained using the FTIR fingerprints (by using Fourier Transform Infrared spectroscopy of raw carotenoid and phenolic extracts. The average concentration of total carotenoids was 54 and 3.9 mg carotenoids/ 100g DW in berries and leaves, respectively. The average concentration of total phenolics was 746 mg GAE/100g DW in berries, approximately 1.8 times lower than total phenolics found in leaves. By PCA (Principal Component Analysis of fingerprints (900-1800 cm-1, the responsible bands for samples discrimination were identified. In case of total carotenoids extract the biomarker bands were: 1745, 1743, 1500 cm-1 for berries and 1458 cm-1 and 1735 cm-1 for leaves, while for total phenolic extract the key bands were 1731, 1033, 1622 cm-1 for berries and 1047 cm-1, 1616, 1512 and 1454 cm-1 for leaves. FTIR spectroscopy proved to be a simple and sensitive analytical technique that can be successfully used in sample discrimination and classification.

  16. Determination of optimum harvesting time for vitamin C, oil and mineral elements in berries sea buckthorn (hippophae rhamnoides)

    International Nuclear Information System (INIS)

    Sea buck thorn a magic plant from Northern areas of Pakistan has multiple uses against various ailments, soil enrichment and environmental purposes. The fruit berries are rich in vitamin C, vitamin A, vitamin E, essential oil, Phytosterol and minerals (Fe, Ca, P, Mn and K). The micro nutrient like vitamin C, oil, phosphorus, magnesium and calcium contents in fruit berries of cultivated sea buck thorn (Hippophae rhamnoides. L. spp. sinensis) harvested at three ripening times were determined using biochemical analysis techniques. Harvesting at different stages of fruit ripening was the primary factor determining maximum expression of these biochemical constituents. Biochemical contents were determined at three fruit developmental stages i.e., unripened stage, medium stage and at full-ripened stage. During this study a decline in vitamin C contents was observed along with the fruit ripening. The oil contents in both seed and pulp increased with fruit ripening. Similarly, the mineral contents like magnesium, calcium and phosphorus contents increases with the fruit ripening in sea buck thorn. The main idea was to identify the maximum expression of biochemical at different stages of fruit maturity. It is concluded that it is better to harvest fruit berries at medium stage of fruit ripening when maximum vitamin C is present. For maximum oil and mineral contents fruit must be harvested at ripening stage. The fruit mesocarp is the area where all genes related with micro nutrients are active at one time i.e., when fruit is maturing, hence the characterization of gene expression activities at this stage may help in the isolation of these genes for future commercial use. (author)

  17. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns

    OpenAIRE

    Nitin K. Upadhyay; Ratan Kumar; Siddiqui, M. S.; Asheesh Gupta

    2011-01-01

    The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD) ointment was used as reference control. The most effective concentration of the extract was found to...

  18. UHPLC/PDA–ESI/MS Analysis of the Main Berry and Leaf Flavonol Glycosides from Different Carpathian Hippophaë rhamnoides L. Varieties

    NARCIS (Netherlands)

    Pop, R.M.; Socaciu, C.; Pintea, A.; Buzoianu, A.D.; Sanders, M.G.; Gruppen, H.; Vincken, J.P.

    2013-01-01

    Introduction - Sea buckthorn (Hippophaë rhamnoides L.) is known to be rich in many bioactive compounds (such as vitamins, phenolics, carotenoids) important for human health and nutrition. Among the phenolics, berries and leaves contain a wide range of flavonols that are good quality and authenticity

  19. Evaluation of antioxidant activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To investigate the antioxidant activity of aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane extracts of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Antioxidant activity was evaluated by using in-vitro antioxidant assays model 1, 1’-diphenyl-2-picrylhydrazyl radical-scavenging activity. The antioxidant activities were compared with standard antioxidant agents such as ascorbic acid. Results: The antioxidant activities (% inhibition) of all the tested extracts were increased in the order i.e. menthol > ethanol > aqueous > acetone > chloroform > ethyl acetate > n-hexane. The methanol extract EC50 (µg/mL) value was compatible with vitamin C (standard). The antioxidant activity of H. rhamnoides leaves extracts increased in a dose dependent manner. Conclusions: It was observed that H. rhamnoides was a potential resource of antioxidants and thus could put off numerous radical linked diseases.

  20. Evaluation of antioxidant activities of Hippophae rhamnoides Linn leaves extracts

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    Javid Ali

    2015-05-01

    Full Text Available Objective: To investigate the antioxidant activity of aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane extracts of Hippophae rhamnoides (H. rhamnoides leaves. Methods: Antioxidant activity was evaluated by using in-vitro antioxidant assays model 1, 1’-diphenyl-2-picrylhydrazyl radical-scavenging activity. The antioxidant activities were compared with standard antioxidant agents such as ascorbic acid. Results: The antioxidant activities (% inhibition of all the tested extracts were increased in the order i.e. menthol > ethanol > aqueous > acetone > chloroform > ethyl acetate > n-hexane. The methanol extract EC50 (µg/mL value was compatible with vitamin C (standard. The antioxidant activity of H. rhamnoides leaves extracts increased in a dose dependent manner. Conclusions: It was observed that H. rhamnoides was a potential resource of antioxidants and thus could put off numerous radical linked diseases.

  1. Nouvelles méthodologies d'extraction, de fractionnement et d'identification : application aux molécules bioactives de l'argousier (Hippophae rhamnoides)

    OpenAIRE

    Michel, Thomas

    2011-01-01

    Plant materials constitute an important source of natural bioactive molecules. Characterisation of these molecules requires often multiple, long and expensive development steps. The aim of this PhD thesis consists of innovation and development of new methods of extraction, fractionation and identification of natural metabolites from plants. We used sea buckthorn as plant model (Hippophaë rhamnoides, Elaeagnaceae) an Eurasian thorny shrub known to possess various phytochemical properties and p...

  2. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

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    Javid Ali

    2015-04-01

    Full Text Available Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane of Hippophae rhamnoides (H. rhamnoides leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution were transferred to specific concentration of each extract. Results: The mortality of aqueous extract was 46.7%, methanol extract was 46.7%, ethanolic extract was 50.0%, ethyl acetate was 26.7%, acetone extract was 33.3%, chloroform extract was 40.0% and n-hexane extract was 33.3%. The lowest LD50 was found in methanol extracts (1199.97 µg/mL. Brine shrimp cytotoxicity of tested extracts of H. rhamnoides showed that mortality rate was concentration dependent. Conclusions: It is concluded that bioactive components are present in all leaves extracts of H. rhamnoides, which could be accounted for its pharmacological effects. Thus, the results support the uses of this plant species in traditional medicine.

  3. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane) of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution) were transferred to specific concentration of each extract. Results: The mortality of aqueous extract was 46.7%, methanol extract was 46.7%, ethanolic extract was 50.0%, ethyl acetate was 26.7%, acetone extract was 33.3%, chloroform extract was 40.0% and n-hexane extract was 33.3%. The lowest LD50 was found in methanol extracts (1199.97 µg/mL). Brine shrimp cytotoxicity of tested extracts of H. rhamnoides showed that mortality rate was concentration dependent. Conclusions: It is concluded that bioactive components are present in all leaves extracts of H. rhamnoides, which could be accounted for its pharmacological effects. Thus, the results support the uses of this plant species in traditional medicine.

  4. A new hydrocarbon material based on seabuckthorn ( Hippophae rhamnoides) sawdust: A structural promoter of cobalt catalyst for Fischer-Tropsch synthesis

    Science.gov (United States)

    Pankina, G. V.; Chernavskii, P. A.; Lunin, V. V.

    2016-09-01

    Aspects of the physicochemical properties of a hydrocarbon material based on seabuckthorn ( Hippophae rhamnoides) sawdust are studied. The use of a hydrocarbon material based on sea buckthorn sawdust as a structural promoter of Co/CHip cobalt catalyst in the reaction of CO hydrogenation is shown to require an additional cycling stage in the mode of reduction and oxidation. The resulting mean size of the Co particles is found to be 18-19 nm and is considered acceptable for the synthesis of C5+ liquid hydrocarbons.

  5. The Evaluation of Synergistic Effect of Hippophae rhamnoides and Vitamin E on Growth Performance and Oxidative Stress at Oreochromis niloticus - Linnaeus, 1758

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    Alina Antache

    2014-11-01

    Full Text Available The aim of this research is to evaluate the influence of sea buckthorn (Hippophae rhamnoides and vitamin E on growth performance indicators and oxidative stress at Nile tilapia juvenile, reared in a recirculating aquaculture system. The experiment was conducted six weeks, in triplicate. The experimental variants were: V1 – control, V2 – 1% sea buckthorn / kg feed, V3 – 500mg vitamin E / kg feed and V4 – 1% sea buckthorn supplemented with 500 mg vitamin E / kg feed. During the experiment was performed an intermediary biometric measurement. Oxidative stress analysis consisted in determination of lipid peroxidation (MDA-malondialdehide and total antioxidant capacity (TAC from liver, tissue and gut. Results showed a good evolution of GR, FCR and SGR, during the experiment, in V4 – in which feed was supplemented with sea buckthorn and vitamin E. Based on the results obtained in variant V4, in liver and tissue, the oxidative stress was reduced. Regarding MDA and TAC, between experimental variants, were registered significant differences (p<0.05 at the level of tissue and gut. In conclusion, the research shows that sea buckthorn (1%/kg feed in combination with Vitamin E (500mg/kg feed has a synergistic effect on growth performance indicators and oxidative stress, at Oreochromis niloticus juvenile.

  6. Genomics in development of radiation countermeasure from Hippophae rhamnoides

    International Nuclear Information System (INIS)

    Development of an effective and non-toxic radiation countermeasure has been a global challenge, till date. On one hand single molecules have failed due to unacceptable toxicity and on the other hand herbal drugs, though non-toxic, are generally neglected because of issues such as incomplete understanding on mechanisms of actions, efficacy and quality control. In the conventional drug discovery process, a single active constituent is isolated, purified, and standardized, but herbal drugs have complex composition and multiple targeted actions. The assessment of efficacy and toxicity of herbal drugs is, therefore, not as straight forward as in case of single active compound. The completion of genome projects of human, mouse and many other simpler experimental models has opened multiple newer avenues for evaluating the metabolism, safety and efficacy of herbal drugs. The radioprotective properties of leaves of Hippophae rhamnoides L. (common name Seabuckthorn, family Eleagnaceae) were reported (Bala et al., 2009, J Herbs and Med Plants). Administration of SBL-1 (a coded herbal preparation from Seabuckthorn leaves), before whole body exposure to lethal dose of 60Co-gamma-rays (10 Gy), rendered >90% survivors in mice population, while 100% mortality was observed in non-SBL-1 treated, 60Co-gamma- irradiated (10 Gy) controls. Though, plant H. rhamnoides is popular for its medicinal and neutraceutical values and finds place is in many traditional medicinal systems world over, its prophylactic use as a radiation countermeasure is novel and needed thorough evaluation. To gain in depth understanding on metabolism and safety of SBL-1, the experimental animals were treated with radioprotective concentration of SBL-1. The genome evaluation was performed at different time intervals after the treatment. This talk highlights some of the prominent molecular pathways and genes affected by SBL-1 treatment in experimental animals. Our study suggested that application of genomics

  7. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns

    Directory of Open Access Journals (Sweden)

    Nitin K. Upadhyay

    2011-01-01

    Full Text Available The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae (SBT and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III. The histological examinations and matrix metalloproteinases (MMP-2 and -9 expression also confirmed the healing efficacy of SBT leaf extract. Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue. The SBT also promoted angiogenesis as evidenced by an in vitro chick chorioallantoic membrane model and in vivo up-regulated vascular endothelial growth factor (VEGF expression. The SBT leaf extract had no cytotoxic effect on BHK-21 cell line. In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.

  8. Effects of Ultra-drying on Vigor and Physiological characteristics of Hippophae rhamnoides Seeds

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Studies of ultra-drying and accelerated aging on Hippophae rhamnoides seeds from the arid and semiarid areas of northwest China were conducted. The results showed that H. rhamnoides seeds had higher desiccation endurance. When seeds were desiccated to 26 g/kg, their germination percentage, vigor indices and enzyme activity could be retained. After 30-day accelerated aging, ultra-dried seeds had better anti-aging ability and storability compared to natural dried seeds, and the best choice for the MC of seed ...

  9. [Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation].

    Science.gov (United States)

    Yang, Dan; Yu, Xuan; Liu, Xu; Liu, Jin-liana; Zhang, Shun-xiang; Yu, Ze-qun

    2015-12-01

    The study aimed to assess the effect of different afforestation modes on microbial composition and nitrogen functional genes in soil. Soil samples from a pure Hippophae rhamnoides stand (SS) and three mixed stands, namely, H. rhamnoides and Pinus tabuliformis (SY), H. rhamnoides and Platycladus orientalis (SB), H. rhamnoides and Robinia pseucdoacacia (SC) were selected. The results showed that the total PLFA (TPLFA), bacterial PLFA, gram positive bacterial PLFA (G⁺PLFA) were significantly higher in soil samples from other three stands than those of the pure one. However, no significant difference was found for fungal PLFA among them. The abundance of nifH, amoA, nirK and narG genes were higher in SY and SC than in SS. The TPLFA, G⁺PLFA, gram negative bacterial PLFA (G⁻PLFA), and all of the detected gene abundance were significantly and positively correlated with soil pH, total organic carbon, total nitrogen, ammonium nitrogen and available potassium. Afforestation modes affected indirectly soil microbial composition and functional genes through soil properties. Mixing P. tabuliformis or P. orientalis with H. rhamnoides might be suitable afforestation modes, which might improve soil quality. PMID:27111999

  10. [Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation].

    Science.gov (United States)

    Yang, Dan; Yu, Xuan; Liu, Xu; Liu, Jin-liana; Zhang, Shun-xiang; Yu, Ze-qun

    2015-12-01

    The study aimed to assess the effect of different afforestation modes on microbial composition and nitrogen functional genes in soil. Soil samples from a pure Hippophae rhamnoides stand (SS) and three mixed stands, namely, H. rhamnoides and Pinus tabuliformis (SY), H. rhamnoides and Platycladus orientalis (SB), H. rhamnoides and Robinia pseucdoacacia (SC) were selected. The results showed that the total PLFA (TPLFA), bacterial PLFA, gram positive bacterial PLFA (G⁺PLFA) were significantly higher in soil samples from other three stands than those of the pure one. However, no significant difference was found for fungal PLFA among them. The abundance of nifH, amoA, nirK and narG genes were higher in SY and SC than in SS. The TPLFA, G⁺PLFA, gram negative bacterial PLFA (G⁻PLFA), and all of the detected gene abundance were significantly and positively correlated with soil pH, total organic carbon, total nitrogen, ammonium nitrogen and available potassium. Afforestation modes affected indirectly soil microbial composition and functional genes through soil properties. Mixing P. tabuliformis or P. orientalis with H. rhamnoides might be suitable afforestation modes, which might improve soil quality.

  11. Influence of ambient and enhanced ultraviolet-B radiation on the plant growth and physiological properties in two contrasting populations of Hippophae rhamnoides

    International Nuclear Information System (INIS)

    Two contrasting sea buckthorn (Hippophae rhamnoides L.) populations from low and high altitude regions were employed to investigate the effects of prevailing and enhanced ultraviolet-B (UV-B) radiation on plant growth and physiological properties under a UVB-enhanced/exclusion system. The experimental design included three UV-B regimes, including excluded (-UVB), near-ambient (NA) and enhanced UV-B (+UVB) radiation. Compared with the control (-UVB), NA caused the formation of smaller but thicker plant leaves in both sea buckthorn populations, paralleled with significant increments of carotenoids and UV-absorbing compounds as well as improved water economy. NA also induced more biomass partition from shoot to root, but CO2 assimilation rate (A), photosynthetic area and biomass accumulation were unaffected. The low-altitude population seemed sensitive to +uvB, as indicated by the decreases in total biomass, A and ascorbic acid content (Asa, an antioxidant) compared with NA. However, little +UVB effect occurred on the high-altitude population, and we suggest that the higher tolerance of this population could be associated with its specific morphological and physiological characteristics, such as small but thick leaves and high-level of Asa content, as well as its greater physiological modification in response to NA, e.g., increases in protective compounds (carotenoids and UV-absorbing compounds) and improvement in water economy, in comparison to the low-altitude population, which form an effective adaptation strategy to enhanced UV-B stress

  12. Fruits foraging patterns and seed dispersal effect of frugivorous birds on Hippophae rhamnoides sinensis

    Institute of Scientific and Technical Information of China (English)

    LU Xianwen; SUN Kun; MA Ruijun; ZHANG Hui; SU Xue; WANG Mingli

    2006-01-01

    Behaviors of 18 species of birds eating fruits of Hippophae rhamnoides spp.sinensis were observed from September 2003 to March 2004.Their foraging patterns were found to be very different and Can be divided into five classes:(1)direct swallowing the fruits on crown of the shrubs and sometimes regurgitating seeds soon after;(2)carrying the fruits to their perching sites and swallowing;(3)pecking the fruits from the shrubs to the ground,eating pulp and seeds but leaving pericarp;(4)pecking through the pericarp,eating pulp and leaving pericarp and seeds;(5)pecking through the pericarp on the top of fruits,and only eating seeds.These foraging patterns have different effects on seed dispersal of H.rhamnoides spp.sinensis.The germination experiment of three groups of seeds(seeds from feces,dry fruits and extracted seeds from dry fruits)was carried out.Although ingestion processes of birds had some adverse effects on the seed germination of H.rhamnoides spp.sinensis,the seeds from feces still have a relatively higher germination ratio.H.rhamnoides spp.sinensis provides food to a variety of frugivorous birds.and the birds disperse its seeds.Thus,a mutually beneficial relationship between the bird and the seed is formed.

  13. Comparative antitumor and anti-proliferative activities of Hippophae rhamnoides L. leaves extracts

    Directory of Open Access Journals (Sweden)

    Javid Ali

    2015-03-01

    Full Text Available Objective: To evaluate the antitumor and anti-proliferative activities of methanol, aqueous, acetone, ethyl acetate, ethanol, chloroform and n-hexane extracts of Hippophae rhamnoides leaves. Methods: Antitumor activities were evaluated by using the antitumor potato disc assay by using inoculums (Agrobacterium tumefaciens with three different concentrations of test samples (10, 100 and 1 000 mg/L. Anti-proliferative activity was evaluated by the given method of methyl thiazolyl tetrazolium assay. The concentrations of the extract ranging from 0.039 to 10 mg/mL were tested against HeLa cells. Results: Highest tumors inhibition activity (60.9% and 55.8% was shown by methanol and ethanol extracts, with EC50 values of 424.41 and 434.61 mg/L respectively. At 10 mg/mL, The highest cell inhibition 75.61% was observed in methanol extract and the lowest 36.59% were calculated in n-hexane extract. The difference in tumor and cell inhibition (% may be due to the different concentration of active compounds responsible for antitumor and anti-proliferative activities. All extracts have considerable level of tumor and cell inhibitiory effect in a dose dependent manner. Conclusions: Our finding showed that Hippophae rhamnoides leaves are a potent natural source of antitumor and antiproliferative agent.

  14. Comparative antitumor and anti-proliferative activities ofHippophae rhamnoidesL. leaves extracts

    Institute of Scientific and Technical Information of China (English)

    Javid Ali; Bashir Ahmad

    2015-01-01

    Objective:To evaluate the antitumor and anti-proliferative activities of methanol, aqueous, acetone, ethyl acetate, ethanol, chloroform andn-hexane extracts ofHippophae rhamnoides leaves. Methods: Antitumor activities were evaluated by using the antitumor potato disc assay by using inoculums (Agrobacterium tumefaciens) with three different concentrations of test samples (10, 100 and 1 000 mg/L). Anti-proliferative activity was evaluated by the given method of methyl thiazolyl tetrazolium assay. The concentrations of the extract ranging from 0.039 to 10 mg/mL were tested against HeLa cells. Results: Highest tumors inhibition activity (60.9% and 55.8%) was shown by methanol and ethanol extracts, with EC50 values of 424.41 and 434.61 mg/L respectively. At 10 mg/mL, The highest cell inhibition 75.61% was observed in methanol extract and the lowest 36.59% were calculated inn-hexane extract. The difference in tumor and cell inhibition (%) may be due to the different concentration of active compounds responsible for antitumor and anti-proliferative activities. All extracts have considerable level of tumor and cell inhibitiory effect in a dose dependent manner. Conclusions:Our finding showed thatHippophae rhamnoidesleaves are a potent natural source of antitumor and antiproliferative agent.

  15. Survival response of hippocampal neurons under low oxygen conditions induced by Hippophae rhamnoides is associated with JAK/STAT signaling.

    Directory of Open Access Journals (Sweden)

    Manimaran Manickam

    Full Text Available Janus activated kinase/signal transducers and activators of transcription (JAK/STATs pathway are associated with various neuronal functions including cell survival and inflammation. In the present study, it is hypothesized that protective action of aqueous extract of Hippophae rhamnoides in hippocampal neurons against hypoxia is mediated via JAK/STATs. Neuronal cells exposed to hypoxia (0.5% O2 display higher reactive oxygen species with compromised antioxidant status compared to unexposed control cells. Further, these cells had elevated levels of pro-inflammatory cytokines; tumor necrosis factor α and interleukin 6 and nuclear factor κappa B. Moreover, the expression of JAK1 was found to be highly expressed with phosphorylation of STAT3 and STAT5. Cells treated with JAK1, STAT3 and STAT5 specific inhibitors resulted in more cell death compared to hypoxic cells. Treatment of cells with extract prevented oxidative stress and inflammatory response associated with hypoxia. The extract treated cells had more cell survival than hypoxic cells with induction of JAK1 and STAT5b. Cells treated with extract having suppressed JAK1 or STAT3 or STAT5 expression showed reduced cell viability than the cell treated with extract alone. Overall, the findings from these studies indicate that the aqueous extract of Hippophae rhamnoides treatment inhibited hypoxia induced oxidative stress by altering cellular JAK1, STAT3 and STAT5 levels thereby enhancing cellular survival response to hypoxia and provide a basis for possible use of aqueous extract of Hippophae rhamnoides in facilitating tolerance to hypoxia.

  16. Flavonoids from Hippophae Rhamnoides L.subsp.sinensis%中国沙棘果实黄酮成分的研究

    Institute of Scientific and Technical Information of China (English)

    高锦明; 张鞍灵

    1999-01-01

    从中国沙棘Hippophae rhamnoides L. subsp. sinensis Rousi果实中分到3种黄酮化合物,经光谱法和化学反应分别鉴定为槲皮素(quercetin,1),山奈酚(Kaempferol,2)和芦丁(rutin,3).

  17. Spatial distribution of Holcocerus hippophaecolus (Lepidopetera: Cossidae) pupae in a seabuckthorn (Hippophae rhamnoides) stand.

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The seabuckthorn carpenter moth,Holcocerus hippophaecolus,which has a generation time of four years,is recently becoming one of the major pests of the seabuckthorn (Hippophae rhamnoides) in Inner Mongolia,Liaoning,Shanxi,Ningxia and Shaanxi of China (Hua et al.,1990).The larvae of the H.hippophaecolus mainly damage the stems and roots of the seabuckthorn,and the mature larvae pupate in the soil.The spatial distribution of the pupae was analyzed by using biostatistics and geostatistics in order to effectively control the insect and further study the spatial distribution of the population.Results show that most of the pupae (90%) had an eclosion time span from early June to the end of July.The sex ratio of the pupae was nearly 1:1 in the woodland samples.In addition,24.3% of the 971 trees investigated had pupae and it ranged from 0 to 4 per tree within a distance of 1.3 m from the base of the stem.90% of the pupae were aggregated within a distance of 1 m from the base of the stem.The pupae show intense spatial aggregation in the sampled woodland which had an 11.1 m spatial dependence and a 90.7% intensity in the local spatial continuity.Moreover,the population presented an intensive spotted distribution and many aggregated spots were found in the woodlands.As for the relationship between grid size and variogram of the pupae,the variations in the range,the intensity of local spatial continuity and the sill were all very low or non-existent when the grid size was 5 m,6 m or 7 m.Whereas,the value of the decisive coefficient was the biggest when the grid size was 5 m making it the ideal grid size.

  18. Characteristics of Caragana korshinskii and Hippophae rhamnoides stemflow and their significance in soil moisture enhancement in Loess Plateau, China

    Institute of Scientific and Technical Information of China (English)

    ShengQi JIAN; ChuanYan ZHAO; ShuMin FANG; Kai YU

    2014-01-01

    Stemflow of xerophytic shrubs represents a significant component of water replenishment to the soil-root system and influences water utilization of plant roots at the stand scale, especially in water-scarce semi-arid ecosystems. The stemflow of two semi-arid shrubs (Caragana korshinskii and Hippophae rhamnoides) and its effect on soil moisture enhancement were evaluated during the growing season of 2011 in the semi-arid loess region of China. The results indicated that stemflow averaged 12.3%and 8.4%of the bulk precipitation for C. korshinskii and H. rhamnoides, respectively. Individual stemflow increased in a linear function with increasing rain-fall depth. The relationship between funneling ratios and rainfall suggested that there existed a rainfall depth threshold of 11 mm for both C. korshinskii and H. rhamnoides. Averaged funneling ratios were 156.6±57.1 and 49.5±30.8 for C. korshinskii and H. rhamnoides, respectively, indicating that the canopy architecture of the two shrubs was an effective funnel to channel stemflow to the root area, and C. korshinskii showed a greater potential to use stemflow water in the semi-arid conditions. For individual rainfall events, the wetting front depths were ap-proximately 2 times deeper in the rooting zone around the stems than in the bare area outside canopy for both C. korshinskii and H. rhamnoides. Correspondingly, soil water content was also significantly higher in the root area around the shrub stem than in the area outside the shrub canopy. This confirms that shrub stemflow conserved in the deep soil layers may be an available moisture source for plant growth under semi-arid conditions.

  19. Total flavones of Hippophae rhamnoides promotes early restoration of ultimate stress of healing patellar tendon in a rat model.

    Science.gov (United States)

    Fu, S C; Hui, C W C; Li, L C; Cheuk, Y C; Qin, L; Gao, J; Chan, K-M

    2005-05-01

    Traditional Chinese herbal medicine has long been used for treatment of tendon injuries. Comparing to the modern way of treatments, Traditional Chinese medicine also stresses on strategies to promote the inherent healing capacity of tendons. Hippophae rhamnoides, known as Shaji, is one of Chinese herbal drugs that are traditionally used to promote tendon and ligament injuries. The total flavones of H. rhamnoides (TFH), with major constituents including quercetin, isorhamnetin and kaempferol, have been demonstrated with most of the bioactive properties of Shaji. In the present study, we evaluated the potential effect of TFH in the restoration of ultimate stress of healing patellar tendon in a well-established gap wound model in rats. A 0.1 mg TFH was injected to wound 1 day after the injury, and the ultimate stress of the healing tendon was measured at day 14 post-injury. The results showed that the ultimate stress of the healing tendon was significantly promoted by injection of TFH, increasing from 30 to 50% as compared to saline control. Excessive fibrotic response was not found in TFH-treated animals, but an enhanced collagen deposition and a better fibre alignment were observed. The results suggest that TFH may improve the ultimate stress of healing tendons at early stages, which implies possible earlier rehabilitation programme and better recovery. PMID:15823472

  20. Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E.

    Science.gov (United States)

    Suleyman, Halis; Gumustekin, Kenan; Taysi, Seyithan; Keles, Sait; Oztasan, Nuray; Aktas, Omer; Altinkaynak, Konca; Timur, Handan; Akcay, Fatih; Akar, Sedat; Dane, Senol; Gul, Mustafa

    2002-09-01

    The aim of this study was to determine the effects of Hippophae rhamnoides L. extract (HRe-1) and also vitamin E as a positive control on nicotine-induced oxidative stress in rat blood, specifically alterations in erythrocyte malondialdehyde (MDA) level, activities of some erythrocyte antioxidant enzymes, and plasma vitamin E and A levels. The groups were: nicotine (0.5 mg/kg/d, intraperitoneal, i.p.); nicotine+vitamin E (75 mg/kg/d, intragastric, i.g.); nicotine+HRe-1 (1 ml/kg/d, i.g.); and control group (receiving only vehicles). There were 8 rats per group and the supplementation period was 3 weeks. Nicotine-induced increase in erythrocyte MDA level was prevented by both HRe-1 and vitamin E. Nicotine-induced decrease in erythrocyte superoxide dismutase (SOD) activity was prevented by HRe-1, but not vitamin E. HRe-1 increased the erythrocyte glutathione peroxidase (GSH-Px) activity compared with nicotine and the vitamin E groups. Catalase activity was not affected. Vitamin E supplementation increased plasma vitamin E level. Plasma vitamin A level was higher in both vitamin E and HRe-1 supplemented groups compared with nicotine and control groups. The results suggest that HRe-1 extract can be used as a dietary supplement, especially by people who smoke, in order to prevent nicotine-induced oxidative stress. PMID:12230103

  1. Protective Effect of Total Flavonoids of Seabuckthorn (Hippophae rhamnoides in Simulated High-Altitude Polycythemia in Rats

    Directory of Open Access Journals (Sweden)

    Sheng-Ya Zeng

    2012-09-01

    Full Text Available Seabuckthorn (Hippophae rhamnoides L. has been used to treat high altitude diseases. The effects of five-week treatment with total flavonoids of seabuckthorn (35, 70, 140 mg/kg, ig on cobalt chloride (5.5 mg/kg, ip- and hypobaric chamber (simulating 5,000 m-induced high-altitude polycythemia in rats were measured. Total flavonoids decreased red blood cell number, hemoglobin, hematocrit, mean corpuscular hemoglobin levels, span of red blood cell electrophoretic mobility, aggregation index of red blood cell, plasma viscosity, whole blood viscosity, and increased deformation index of red blood cell, erythropoietin level in serum. Total flavonoids increased pH, pO2, SpO2, pCO2 levels in arterial blood, and increased Na+, HCO3−, Cl−, but decreased K+ concentrations. Total flavonoids increased mean arterial pressure, left ventricular systolic pressure, end-diastolic pressure, maximal rate of rise and decrease, decreased heart rate and protected right ventricle morphology. Changes in hemodynamic, hematologic parameters, and erythropoietin content suggest that administration of total flavonoids from seabuckthorn may be useful in the prevention of high altitude polycythaemia in rats.

  2. 微波法提取沙棘总黄酮的工艺研究%Technical Study of the Microwave Extraction of the Total Flavonoids from Hippophae Rhamnoides

    Institute of Scientific and Technical Information of China (English)

    李宋玲

    2012-01-01

    目的 研究微波法提取沙棘总黄酮的最佳提取条件.方法 首先用石油醚对沙棘进行脱脂处理,然后在单因素试验基础上,以乙醇浓度、微波功率、提取时间、料液比为因素,选取不同水平,以沙棘总黄酮提取率为评价指标,通过正交试验筛选出最佳提取工艺.结果 实验选取的四个因素中,乙醇浓度和料液比对沙棘总黄酮提取率影响显著;微波法提取沙棘总黄酮的最佳提取工艺条件为乙醇浓度75%,微波功率300 W,提取时间2 min,料液为1:20,沙棘总黄酮提取率最高可达3.9%.结论 微波法提取沙棘总黄酮有时间短、提取效率高、对沙棘活性成分破坏较少等优点,可以为沙棘资源的综合开发提供参考.%Objective To study the optimal extraction condition of the flavones from Hippophae Rh-amnoide with microwave method. Methods The petroleum ether was used for the derosination of Hippophae Rhamnoides at first. Afterward,on the basis of the single -factor test, by taking ethanol concentration,microwave power, extraction time and feed liquid ratio as the factors, at different levels, taking the extraction rate of the total flavonoids from Hippophae Rhamnoides as the assessment indicator, the orthogonal test was applied to optimize the extraction techniques. Results Of the four factors optioned in the trial, the ethanol concentration, and feed liquid ratio presented the significant impacts on the extraction rate of the total flavonoids from Hippophae Rhamnoides. The impacts of microwave power and extraction time on the trial results were not significant. The optimal extraction technical conditions of the total flavonoids from Hippophae Rhamnoides were 75% of ethanol concentration,300 w in microwave power,2 min in the extraction time and 1: 20 in feed liquid ratio. The extraction rate of the total flavonoids from Hippophae Rhamnoides could be up to 3. 9%. Conclusion The microwave extraction method is advantageous as short

  3. Effect of Sea buckthorn on liver fibrosis: A clinical study

    Institute of Scientific and Technical Information of China (English)

    Ze-Li Gao; Xiao-Hong Gu; Feng-Tao Cheng; Fo-Hu Jiang

    2003-01-01

    AIM: To appraise the effect of sea buckthorn (Hippophae rhamnoides) on cirrhotic patients.METHODS: Fifty cirrhotic patients of Child-Pugh grade A and B were randomly divided into two groups: Group A as the treated group (n=30), taking orally the sea buckthom extract, 15 g 3 times a day for 6 months. Group B as the control group (n=18), taking vitamin B complex one tablet,3 times a day for 6 months. The following tests were performed before and after the treatment in both groups to determine LN, HA, collagens types Ⅲ and IV, cytokines IL6 and TNFα, liver serum albumin, total bile acid, ALT, AST and prothrombin time.RESULTS: The serum levels of TNFα, IL-6, laminin and type IV collagen in group A were significantly higher than those in the control group. After a course of sea buckthorn treatment, the serum levels of LN, HA, collagen types Ⅲand IV, total bile acid (TBA) decreased significantly as compared with those before and after treatment in the control group. The sea buckthorn notably shortened the duration for normalization of aminotransferases.CONCLUSION: Sea buckthom may be a hopeful drug for prevention and treatment of liver fibrosis.

  4. Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula

    Directory of Open Access Journals (Sweden)

    Barkat Ali Khan

    2013-12-01

    Full Text Available The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®. The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use.

  5. Pharmacokinetic properties of isorhamnetin, kaempferol and quercetin after oral gavage of total flavones of Hippophae rhamnoides L. in rats using a UPLC-MS method.

    Science.gov (United States)

    Li, Guowen; Zeng, Xiaoli; Xie, Yan; Cai, Zhenzhen; Moore, Jeffrey C; Yuan, Xiurong; Cheng, Zhihong; Ji, Guang

    2012-01-01

    An ultra performance liquid chromatography-mass spectrometric (UPLC-MS) method was developed to investigate the pharmacokinetic properties of isorhamnetin, kaempferol and quercetin from a total flavone extract of Hippophae rhamnoides L. (TFH) after single dose oral administration. Rat plasma samples were pretreated using liquid-liquid extraction, and chromatographic separation was performed on a C(18) column using a linear gradient of methanol and formic acid (0.1%). The pharmacokinetic parameters of isorhamnetin, kaempferol and quercetin from TFH in rats were quantitatively determined by UPLC with photodiode array detection (PDA). The qualitative detection of the three flavones was accomplished by selected ion monitoring in negative ion mode ESI-MS. Results of the pharmacokinetic study indicate that the three flavones in TFH were absorbed by passive diffusion in rats, and no "double-peak" phenomenon was observed in C-t curves of the three flavones from TFH except for quercetin. Results of this study indicate that the pharmacokinetic behaviors of isorhamnetin, kaempferol and quercetin when administered together in a complex herbal extract might be different than the individual behaviors of the same compounds administered in their pure forms. Results of this study also demonstrate that UPLC-MS is a rapid and practical method to determine the pharmacokinetic parameters of flavones present in an herbal extract. PMID:22056665

  6. Characteristics of root distribution of Hippophae rhamnoides L.and its improving effect on the forest soil on the ditch slope of soft sandstone area%砒砂岩沟坡沙棘根系分布特征及其对林下土壤的改良作用

    Institute of Scientific and Technical Information of China (English)

    党晓宏; 高永; 汪季; 罗凤敏; 高君亮; 刘阳

    2012-01-01

    沙棘是砒砂岩地区植被恢复的先锋树种,沙棘根系形态不但影响植物本身的生长,还影响其保土效果及改土效果.采用根系追踪法,研究砒砂岩地区同一坡面不同坡位、不同林龄沙棘根系的分布形态,测定沙棘林下土壤的理化性质,以裸坡地作为对照,分析沙棘林对土壤的改良作用.结果表明:1)砒砂岩沟坡沙棘根系在垂直方向上集中分布在0~40 cm深的土壤中,在水平方向分布可达137 cm.2)沙棘一级侧根的数量与小环境有密切关系,土壤水分条件越好,侧根数量越多,根长越短;沙棘根系的径粗与埋深呈反比,并且与距植株中心距离也呈反比.3)人工沙棘林在砒砂岩沟坡上,其根系分布表现为坡顶根系由于土层薄受到砒砂岩的限制,水平分布较发达,在坡底由于土壤厚度及肥力的充足,沙棘在垂直分布上明显深于坡顶.4)种植沙棘可增加土壤含水量和土壤孔隙度,同时还可以提高土壤中的速效N、P、K及有机质质量含量.%Hippophae rhamnoides L. Is one of pioneer tree species in soft sandstone area. The distribution patterns of roots affect not only the growth of the plant itself, but also soil conservation and soil improving. The paper studied the root distribution patterns of different plantation age of Hippophae rhamnoides L. In three site conditions on slopes using roots tracing method. Through measuring the soil physical and chemical properties in Hippophae rhamnoides L. Forests, and compared with that on bare slopes, the improving effect of planting Hippophae rhamnoides L. On forest soil was analyzed. The results of the study show that: roots of Hippophae rhamnoides L. Mainly distributed in the depth from 0 to 40 cm in soil on ditch slope in the soft sandstone area, in horizontal direction the roots could reach 137 cm. There was a close relationship between the numbers of first lateral root of Hippophae rhamnoides L. And microenvironment. If

  7. Proximate composition, mineral contents, phytochemical constituents, antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides Linn

    Institute of Scientific and Technical Information of China (English)

    Javid Ali; Bashir Ahmad; Said Hassan; Muhammad Siddique; Farrah Gul; Shafaat Ullah

    2015-01-01

    Objective: To compare the proximate composition, mineral contents, antimicrobial, phytochemical and Fourier transforms infrared (FTIR) spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides. Methods: Proximate composition was determined according to the described methods. Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer. Antimicrobial activities were evaluated according to the agar well diffusion method. Phytochemical qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu Japan. Results:The proximate analysis showed high content of protein and fiber in stem and bark. High content of Na (900 mg/L) and K (670 mg/L) was found in bark powder, while in seed, high contents of Ca (800 mg/L), Mg (725 mg/L), Fe (250 mg/L) Zn (90 mg/L) and Mn (65 mg/L) were found compared to stem and bark. Phenols, flavonoids and tannins showed high contents in stem and bark of all extracts. The bark aqueous extract showed high zone of inhibition against Staphylococcus aureus (21 mm) and Escherichia coli (20 mm), while methanol extract of stem showed high zone of inhibition (14 mm and 13 mm) against Enterococcus faecalis and Escherichia coli respectively. The aqueous extract of bark documented high zone of inhibition against Aspergillus niger (21 mm) and Aspergillus parasiticus (20 mm). FTIR spectra revealed the presence of OH, C-O and C=O functional groups. Conclusions:The study concludes that bark, stem and seed extracts will be useful guideline for the new syntheses of feed, food supplements and herb drugs with various combination, which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.

  8. Proximate composition, mineral contents, phytochemical constituents, antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides Linn

    Directory of Open Access Journals (Sweden)

    Javid Ali

    2015-06-01

    Full Text Available Objective: To compare the proximate composition, mineral contents, antimicrobial, phytochemical and Fourier transforms infrared (FTIR spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides. Methods: Proximate composition was determined according to the described methods. Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer. Antimicrobial activities were evaluated according to the agar well diffusion method. Phytochemical qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu Japan. Results: The proximate analysis showed high content of protein and fiber in stem and bark. High content of Na (900 mg/L and K (670 mg/L was found in bark powder, while in seed, high contents of Ca (800 mg/L, Mg (725 mg/L, Fe (250 mg/L Zn (90 mg/L and Mn (65 mg/L were found compared to stem and bark. Phenols, flavonoids and tannins showed high contents in stem and bark of all extracts. The bark aqueous extract showed high zone of inhibition against Staphylococcus aureus (21 mm and Escherichia coli (20 mm, while methanol extract of stem showed high zone of inhibition (14 mm and 13 mm against Enterococcus faecalis and Escherichia coli respectively. The aqueous extract of bark documented high zone of inhibition against Aspergillus niger (21 mm and Aspergillus parasiticus (20 mm. FTIR spectra revealed the presence of OH, C-O and C=O functional groups. Conclusions: The study concludes that bark, stem and seed extracts will be useful guideline for the new syntheses of feed, food supplements and herb drugs with various combination, which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.

  9. Anti-mutagenic Effect of Water-soluble Polysaccharide from Hippophae rhamnoides Leaves%沙棘叶水溶性多糖的抗突变作用

    Institute of Scientific and Technical Information of China (English)

    李芳亮; 高杨; 刘莹; 杨新源; 王锐

    2011-01-01

    In order to explore the anti-mutagenic effect of water-soluble polysaccharides from Hippophae rhamnoides leaves(WPHL),the inhibitory effect of WPHL on bone mouse marrow cell micronuclei,abnormal sperm and hepatic DNA damage induced by cyclophosphamide(CP) was studied.The results show that WPHL at the dose of 50 mg/kg·d significantly inhibited the frequency of micronucleus(P 0.05) and sperm abnormality(P 0.05).In addition,WPHL could also significantly decrease the number and tail length of hepatic comet cells(P 0.05).Therefore,WPHL has anti-mutagenic potential.%为了研究沙棘叶水溶性多糖(WPHL)的抗突变作用,本实验通过微核实验、精子畸变实验和彗星实验观察WPHL对环磷酰胺(CP)诱发的小鼠骨髓嗜多染红细胞微核、肝细胞DNA损伤和精子畸变的抑制作用。结果表明:WPHL在50mg/(kg.d)剂量时,对CP诱发的微核(P〈0.05)和精子畸变(P〈0.05)有明显的抑制作用,并可以显著减小肝脏彗星细胞的数量和缩短彗星细胞尾巴的长度(P〈0.05)。说明有显著的抗突变能力。

  10. Hippophae rhamnoides L.leaves extract enhances cell proliferation and neuroblast differentiation through upregulation of intrinsic factors in the dentate gyrus of the aged gerbil

    Institute of Scientific and Technical Information of China (English)

    Ji Hyeon Ahn; Bai Hui Chen; Joon Ha Park; In Hye Kim; Jeong-Hwi Cho; Jae-Chul Lee; Bing Chun Yan

    2014-01-01

    Background Hippophae rhamnoides L.(HL) exerts antioxidant activities against various oxidative stress conditions.In this study,we investigated effects of extract from HL leaves (HLE) on cell proliferation and neuroblast differentiation in the subgranular zone (SGZ) of the dentate gyrus (DG) of aged gerbils.Methods Aged gerbils (24 months) were divided into vehicle (saline)-treated-and HLE-treated-groups.The vehicle and HLE were orally administered with 200 mg/kg once a day for 20 days before sacrifice.Cell proliferation and neurobiast differentiation were examined in the DG using Ki67 and doublecortin (DCX),respectively.We also observed changes in immunoreactivities of superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2),brain-derived neurotrophic factor (BDNF),and phospho-glycogen synthase kinase-3-beta (p-GSK-3β) to examine their relation with neurogenesis using immunohistochemistry.Results The administration of HLE significantly increased the number of Ki67-positive cells and DCX-positive neuroblasts with well-developed processes in the SGZ of the DG of the HLE-treated-group.In addition,immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in granule and polymorphic cells of the DG in the HLE-treated-group compared with those in the vehicle-treated-group.Conclusions HLE treatment significantly increased cell proliferation and neuroblast differentiation,showing that immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in the DG.These indicate that increased neuroblast differentiation neurogenesis may be closely related to upregulation of SOD1,SOD2,BDNF,and p-GSK-3β in aged gerbils.

  11. [Determination of plasma concentration of quercetin, kaempferid and isorhamnetin in Hippophae rhamnoides extract by HPLC-MS/MS and pharmacokinetics in rats].

    Science.gov (United States)

    Liu, Yu; Yang, Juan; Tuo, Yang-ling; Wei, Ting; Zeng, Yong; Wang, Ping; Meng, Xian-li

    2015-10-01

    To establish an HPLC-MS/MS method for the analysis of quercetin, kaempferid and isorhamnetin in rats plasma and study its pharmamacokinetics after an intragastrical administration of Hippophae rhamnoides extracts. Five healthy male Sprague-Dawley (SD) rats were given single doses of H. rhamnoides extracts (quercetin 26.35 mg x kg(-1), kaempferid 4.040 mg x kg(-1), isorhamnetin 31.37 mg x kg(-1)), and then their orbital sinus blood samples were collected at different time points. The drug plasma concentration of the three flavonoids was determined by HPLC-MS/MS method. After that, the main pharmacokinetics parameters were calculated by using Kinetica 5. 0. 11 software. The methodological test showed that the linear concentration ranges of quercetin, kaempferid and isorhamnetin were 7.500-600.0 μg x L(-1) (R2 = 0.998 5), 1.000-80.00 μg x L(-1) (R2 = 0.998 5 ) and 10.00-800.0 μg x L(-1) (R2 = 0.998 0), respectively. The inner and inter-days precisions were both less than 14.0%. The plasma samples showed a good stability and consistency with the requirement of biological sample analysis after the samples were frozen once and placed at - 20 degrees C for 15 d and room temperature for 6 h and the treated analytes were placed at -20 degrees C for 24 h. For quercetin, the pharmacokinetic parameter t(½β), AUC(0-∞), MRT(0.∞), C.(max) and T(max) were (113.3 ± 19.37) min, (12 542.14 ± 3 504.05) μg x h x L(-1), (119.6 ± 13.29) h, (164.6 ± 27.33) μg x L(-1) and (5.199 ± 0.840 3) h, respectively. For kaempferid, the pharmacokinetic parameters t(½β), AUC(0-t), MRT(0-∞), C(max) and T(max) were (79.85 ± 17.15) min, (934.51 ± 94.59) μg x h x L(-1), (81.50 ± 13.75) h, (80.15 ± 14.24) μg x L(-1) and (3.827 ± 0.902 7) h, respectively. For isorhamnetin, the pharmacokinetic parameters t1,2,, AUC(0-t), MRT(0-∞), C(max) and T(max) were (118.3 ± 20.73) min, (26 067.77 ± 4 124.60) μg x h x L(-1), (129.0 ± 16.30) h, (269.6 ± 29.32) μg x L(-1) and (6.513 ± 1

  12. Effects of Total Flavonoids of Hippophae Rhamnoides L. on Intracellular Free Calcium in Cultured Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats and Wistar-Kyoto Rats

    Institute of Scientific and Technical Information of China (English)

    ZHU Fu; CHEN Wei; HUANG Pan-hua; HUANG Bo; HU Chun-yan; JIANG Qing-yuan; LU Zhen-guo; LU Ming; WANG Mei-hua; GONG Min; QIAO Chun-ping

    2005-01-01

    Objective: To explore the effects of total flavonoids of Hippophae rhamnoides L. (TFH),quercetin (Que) and isorhamnetin (Isor) on the intracellular free calcium ([Ca2+ ]i) in vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Methods: Fluo 3-acetoxymethylester(Fluo-3/AM) was used to observe the effects of TFH (100mg/L) and its essential monomers, namely Que (10-4mol/L) and Isor (10-4mol/L) on changes of [Ca2+] i in cultured SHR and WKY VSMC (abbr. to Ca-SHR & Ca-WKY) following exposure to high K+, norepinephrine (NE) and angiotensin Ⅱ (Ang Ⅱ ), and to compare with the effects of verapamil (Ver). Results: (1) TFH, Que and Isor had inhibitory effects on resting Ca-SHR (P<0.05), but had no significant effects on Ca-WKY (P>0.05). (2) High K+ could increase Ca-SHR more significantly than Ca-WKY (P<0.05); TFH, Que and Isor could inhibit the elevation of [Ca2+ ]i induced by high K+-depolarization, with the effects similar to that of Ver, and the effect on Ca-SHR was more significant than that on Ca-WKY ( P<0.05). (3) NE and Ang Ⅱ could increase Ca-SHR more significantly than Ca-WKY ( P<0.05), TFH, Que and Isor had remarkably inhibitory effect on the elevation of Ca-SHR and Ca-WKY induced by NE or Ang Ⅱ. (4) In the absence of extracellular Ca2+ , TFH, Que and Isor also had certain inhibitory effect on Ca-SHR and Ca-WKY induced by NE, and the effect on the former was more significant than that on the latter( P<0.05). Conclusion: TFH, Que and Isor might decrease the levels of [Ca2+ ]i in VSMCs by blocking both voltage-dependent calcium channels (VDC) and receptoroperated calcium channels (ROC) in physiological or pathological state, which may be one of the important mechanisms of their hypotensive and protective effects on target organs in patients with hypertension.

  13. 中国沙棘染色体核型及进化研究%Study on the Chromosome Karyotype and Evolution of Hippophae rhamnoides Linn.ssp.sinensis Roust

    Institute of Scientific and Technical Information of China (English)

    王彬

    2011-01-01

    The chromosome karyotype of Hippophae rhamnoides Linn. ssp. sinensis Rousi was analyzed. The results indicated that the chromosome numher of H. rhamnoides ssp. sinensis was 2n=24. basic number was 12, no aneuploidy and polyploid was observed in the chromosome; The karyotype formula was 2n=24=14M+1Om, no satellites were observed in the chromosome. the relative length of the chromosome composition was 2n=4L+6M2+1OM1+4S, the karyotype belonged to "1B" type, the asymmetry coefficient of karyotype was 54%. Based on the Stebbins' evolution theory of the chromosome karyotype and the method for ordering and assignment of cladistic systematics, four important traits were analyzed. The results indicated that the evolution index of chromosome karyotype of H. rhamnoides ssp. sinensis was three. the evolution extent was low, belonging to the more primitive species; this study provided the cytology theory basis for the development of the resources. breeding and variety classification.%对中国沙棘(Hippophae rhamnoides Linn.ssp.sinensis Rousi)的染色体进行了核型分析,结果表明,中国沙棘染色体数目为2n=24,基数为12,未发现具有非整倍体和多倍体的个体;其核型公式为2n=24=14M+10m,染色体中未发现随体,染色体相对长度组成为2n=4L+6M2+10M1+4S,核型属于"1B"型,核型不对称系数As.K为54%.同时.根据Stebbins的核型进化理论和分支系统学的编序、赋值方法.对核型的4个重要性状进行了分析,结果表明,中国沙棘染色体的进化指数为3,进化程度较低,属于较原始种;这些研究结果为中国沙棘资源的开发、良种的选育和分类提供了细胞学理论依据.

  14. 罗马尼亚不同沙棘品种叶中甘树脂的提取%Foliar Assimilating Pigments in Different Hippophae rhamnoides L . Varieties in the Romanian Flora

    Institute of Scientific and Technical Information of China (English)

    Maria Magdalena Zamfirache; Titu Zamfirache; 梁月; Zenovia Olteanu; Elena Truta; Stefania Surdu; Lacramioara Oprica; Ioan Viorel Rati; Ciprian Manzu; Gurau Milian; Craita Rosu

    2010-01-01

    沙棘(Hippophae rhamnoides)是一种可广泛生长于欧亚地区,包括罗马尼亚的小果实灌木.沙棘具有药用及营养价值,同时可以改善区域的微气候环境.人们已经研究了罗马尼亚沙棘的生物化学组分及相关数据,所以我们分析了不同沙棘叶的甘树脂,一个重要的生化参数可以为植株的一项基本新陈代谢--光合作用提供信息.结果表明根据品种及特定环境因素的不同,产生了多样性.

  15. Extraction Isorhamnetin from Marc of Hippophae rhamnoides L. with Seal Heating Method%密封加热法提取沙棘果渣中异鼠李素

    Institute of Scientific and Technical Information of China (English)

    何珺; 胡丹; 杨佳年

    2015-01-01

    采取密封加热的方法对从沙棘果渣中提取异鼠李素的工艺进行研究。采用单因素实验对提取时间、提取次数、提取温度、料液比及提取溶剂进行筛选,之后对提取条件进行正交验证实验。实验确定了密封加热法提取沙棘果渣中异鼠李素的最佳工艺为:55℃条件下用V 乙酸乙酯∶V95%乙醇=4∶6的提取液水浴加热2次,每次3 h,料液比为1∶20(g∶mL)。%Extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were studied. Single factor experiment was used to select extraction time, extraction times, extraction temperature, solid-liquid ratio and extraction solvent. Then the orthogonal verification experiment was used. The best processes of extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were as follows:under temperature 55℃, Vethyl acetate∶V95%ethyl alcohol were 4∶6 as extraction solvent, extraction time 3 h, material to liquid ratio 1∶20(g∶mL), and extraction times 2.

  16. 泰山沙棘果提取物调血脂作用研究%Study on Regulating Blood Lipid Effect of the Extracts of Taishan Hippophae rhamnoides Fruits

    Institute of Scientific and Technical Information of China (English)

    张颖; 张立木; 秦树存; 李同德; 彭吉星

    2011-01-01

    目的:研究沙棘果醇提物和水提物对高脂血症模型小鼠的调血脂作用.方法:以喂饲普通饲料作为正常对照组,以高脂血症小鼠作为模型组,以1000、500和250 mg·kg-1ig剂量的沙棘果醇提物和水提物为实验组,测定血清中的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)含量.结果:模型组TC、TG水平显著高于正常对照组(P<0.01);与模型组比较,不同剂量沙棘果醇提物可显著降低TC水平(P<0.01);不同剂量沙棘果醇提物与水提物可显著降低TG水平(P<0.05或P<0.01),对高脂血症模型小鼠血清中HDL-C无升高作用.结论:沙棘果提取物具有明显的调血脂作用,可用于高脂血症的预防和治疗.%OBJECTIVE:To explore the effect of the ethanol extract and water extract of Hippophae rhamnoides fruits on regulating blood lipid in hyperlipemia mice. METHODS: Normal control group was treated with common diet, experimental hyperlipemia mice were model group. The hyperlipemia mice were raised with ethanol extract and water extract of H. rhamnoides fruits at the dose of 1 000, 500 and 250 mg·kg-1 as experiment group. The levels of total chholesterin(TC),triglyeride (TG),high lipoprotein cholesterol (HDL-C) in serum were all tested. RESULTS: The level of TC and TG in model group were higher than in normal control group(P<0.01).Compared with model group, the level of the TC decreased significantly in the different dose ethanol extract of H. rhamnoides fruits group (P<0.01). The level of TG decreased significantly in different dose of ethanol extract and water extract of H. rhamnoides fruits (P<0.05 or P<0.01), and it was not obvious effect on the level of HDL-C in serum. CONCLUSION: The extracts of H. rhamnoides fruits have significantly effects on regulating blood lipid. It can prevent and cure the hyperlipemia.

  17. Morphological and physiological response ofHippophae rhamnoides on alkaline salt stress%沙棘对碱性盐胁迫的形态和生理响应

    Institute of Scientific and Technical Information of China (English)

    于畅; 王竞红; 薛菲; 江远芳

    2014-01-01

    采用50、100、150 mmol·L-13种浓度的Na2CO3、NaHCO3及二者混合盐对2年生沙棘幼苗进行胁迫处理,通过比较分析胁迫后沙棘幼苗的形态和细胞膜透性、脯氨酸含量、可溶性糖含量的变化,探讨沙棘对Na2CO3、NaHCO3和混合盐胁迫的适应能力。结果表明:在胁迫条件下,沙棘出现枯黄、落叶等盐害症状,随着胁迫浓度的加大和时间的延长,症状表现逐渐加重;电导率、脯氨酸含量、可溶性糖含量均随胁迫程度的加深呈上升趋势,胁迫前期变化相对稳定,后期变化幅度较大;形态表现和生理指标实验均反映出,不同类型盐碱胁迫对沙棘的伤害程度不同,单盐的迫害作用大于复盐,碱性盐的伤害作用大于酸性盐,即Na2CO3>NaHCO3>混合盐碱。此结果对提高沙棘在东北地区护坡工程中的应用提供了技术支持。%The two-year-oldHippophae rhamnoides seedlings were treated by stress method with three kinds of salts (Na2CO3, NaHCO3, the mixture of Na2CO3 and NaHCO3) under different concentrations (50,100,150 mmol/L). The changes of morphology, cell membrane permeability, proline content and soluble sugar content ofH. rhamnoides seedlings after being stress treatment were compared and analyzed. Thus, the adaptive capacity ofH. rhamnoides seedlings under the stress of Na2CO3, NaHCO3 and the mixture of Na2CO3 and NaHCO3 was studied. The results show that under the condition of stress, the salt damage symptoms toH. rhamnoides seedlings appeared, such as leaf blight-yellow and leaf fell, with the increase of stress concentration and time, the symptoms gradually worsened; the electrical conductivity, proline content, soluble sugar content increased with the trend of stress degree going upward, in the stress earlier stage the change was relatively stable, later changed greatly; the morphology and physiological indicators reflected that the damage degrees of saline alkali stress to

  18. Density and Biomass Dynamics of Hippophae rhamnoides L.subsp.sinensis Population in Mu Us Sandland%毛乌素沙地中国沙棘种群数量动态研究

    Institute of Scientific and Technical Information of China (English)

    李根前; 赵粉侠; 李秀寨; 韦宇

    2004-01-01

    Based on the measurement of all individuals of population at different successional stage and following excavation of clones, the density and biomass dynamics of Hippophae rhamnoides L. subsp, sinensis population in Mu Us sandland were analyzed with the methods of plots arranged with age and reverse age class addition(RAA). The main results were as follows:(1)The density of clone population increased with the population growth before 5-year-old. The population biomass accumulating also increased by Logistic equation in the period of age structure of population developed from increasing type to mid-decreasing type.Then they tended to be decrease. While the population density, population biomass augmented again with the emergence of gap regeneration, and the dominant status of H.rhamnoides L.subsp, sinensis population and the stability of the community were maintained. (2)The decrease process of density of daughter ramets population lagged behind that of mother ramets population,and the numerical ratio of daughter ramets population within the population gradually enlarged. As the population thinned, the composition of population changed from more clones with less daughter ramets to less clones with more daughter ramets and the genetic diversity of population reduced. (3)The prosperous stage of biomass accumulation appeared from 8-year-old to 16-year-old and its peak appeared at 12-year-old. And the relationship between the individual mean weight and population density could be expressed by the law of power-3/2 in the period of age structure of population developed from stable type to decreasing type.

  19. Three-dimensional Shade Seedling Nursing Method of Hippophae Rhamnoides L. Suitable for Arid Desert Area in Xinjiang Autonomous Region%适用于新疆干旱荒漠区的沙棘立体遮荫育苗法

    Institute of Scientific and Technical Information of China (English)

    王东健; 陈奇凌; 庄振刚; 张献辉; 花东来

    2014-01-01

    In particularly bad area with very adverse circumstances in acrid desert region, it is needed to afforest by culturing seedling plant of Hippophae rhamnoides L. with strong stress resistance and good economic benefit. The study showed that 10~15 days before the seabuckthorn sowing planting the early-maturing maize by the side of seabuckthorn rows for the three-dimensional shade seedling nursing with windbreak effect can not only improve the rate of emergence and seedling survival of Hippophae rhamnoides L., but also meet Hippophae rhamnoides L.’ need for strong illumination after the harvest of maize. Compared with regular field seeding and greenhouse nursery, this practice is characterized by high rate of emergence, high growth (about 14cm more than normal nursery), less initial input and high final comprehensive gains (about 3113 yuan increased per mu). So the three-dimensional shade nursing method serves as an effective measure for Hippophae rhamnoides L. seeding and seedling nursing in ar-id desert area.%在新疆干旱荒漠区的环境特别恶劣地段,需要培育抗逆性强且经济效益较好的沙棘实生苗造林。经试验研究,在沙棘播种前10~15d于沙棘行边稀疏地播种早熟玉米,进行沙棘立体遮荫挡风育苗,既能提高沙棘出苗率和保苗量,又能在后期玉米成熟砍去后,满足沙棘对强光照的需要。这一方法比起大田常规育苗和固定温棚育苗,出苗率高,苗高较常规育苗提高14cm ,且前期投入少,后期综合收益大,亩效益较常规育苗提高3113元。立体遮荫育苗法,是适用于新疆干旱荒漠区沙棘播种育苗的有效措施。

  20. Two new flavonoid glycosides isolated from seed residue of Hippophae rhamnoides ssp. sinensis%中国沙棘籽粕中2个新的黄酮苷类化合物

    Institute of Scientific and Technical Information of China (English)

    高雯; 张静; 陈超; 孔德云

    2013-01-01

    目的 对中国沙棘Hippophae rhamnoides ssp.sinensis籽粕的化学成分进行研究.方法 采用多种色谱方法进行分离纯化,运用多种谱学方法鉴定化合物的结构.结果 从中国沙棘籽粕的70%乙醇提取物中分离得到3个化合物,分别鉴定为山柰酚-3-O-[(2E-2,6-二甲基-6-羟基-2,7-辛二烯酰-(1→6)]-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃葡萄糖苷-7-O-[(2E)-2,6-二甲基-6-羟基-2,7-辛二烯酰-(1→3)]-α-L-吡喃鼠李糖苷(1)、山柰酚-3-O-[(2E)-2,6-二甲基-6-羟基-2,7-辛二烯酰-(1→6)]-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃葡萄糖苷-7-O-[(2E)-2,6-二甲基-6-羟基-2,7-辛二烯酰-(1→2)]-α-L-吡喃鼠李糖苷(2)、异鼠李素-3-O-β-D-芸香糖苷(3).结论 化合物1和2均为新的黄酮苷,分别命名为沙棘黄酮苷A和沙棘黄酮苷B .%Objective To study the chemical constituents in the seed residue of Hippophae rhamnoides ssp. sinensis. Methods Various chromatographic methods were employed to isolate the compounds and their structures were established by detailed spectroscopic analysis. Results Three compounds were isolated from 70% ethanol extract from the seed residue of H. rhamnosides ssp. sinensis and identified as kaempferol-3-O-[(2E)-2, 6-dimethyl-6-hydroxy-2, 7-octadienoyl-(1→6)]-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside-7-O-[(2E)-2, 6-dimethyl-6-hydroxy-2, 7-octadienoyl-(1→3)]-α-L-rhamnopyranoside (1), kaempferot-3-0-[(2E)-2,6-dimethyl-6-hydroxy-2, 7-octadienoyl-( 1→6)]-β-D-glucopyranosyl-(1→2)- β-D-glucopyranoside-7-0-[(2E)-2, 6-dimethyl-6-hydroxy-2, 7-octadienoyl(1→2)]-α-i-rhamnopyranoside (2), and isorhamnetin-3-O-β-D-rutinoside (3). Conclusion Compounds 1 and 2 are new flavonoid glycosides, named seabuckthom flavonoid glycoside A and seabuckthom flavonoid glycoside B, respectively.

  1. Clinical observation of compound Hippophae Rhamnoides seed oil suppository combined with An’ erdian in treatment of bacterial vaginosis%复方沙棘籽油栓联合安尔碘Ⅲ型消毒剂治疗细菌性阴道病的临床观察

    Institute of Scientific and Technical Information of China (English)

    肖夏; 孙倩

    2015-01-01

    Objective It is to observe the clinical efficacy on bacterial vaginosis treated by Compound Hippophae Rham -noides seed oil suppository combined with An ’ erdian Ⅲ disinfectant .Methods 185 patients were diagnosed with bacterial vaginosis in clinic .According to the treatment time , the patients were divided into 3 groups randomly .Group A was treated with metronidazole orally combined with putting compound Hippophae Rhamnoides seed oil suppository into vagina .Group B was treated with compound Hippophae Rhamnoides seed oil suppository into vagina and disinfecting the vagina and vulva with An’erdian Ⅲdisinfectant.Group C was treated with compound Hippophae Rhamnoides seed oil suppository into vagina , dis-infecting the vagina and vulva with An ’ erdianⅢdisinfectant combined with metronidazole orally .The clinical symptoms , com-prehensive therapeutic effects , laboratory examinations , the recurrence rates and drug safety of those groups were compared . Results The comprehensive therapeutic effects , improvements of vaginal pH , normal rates of ammonia transfer , and improve-ments of clinical symptoms after treatment in group B and C were better than that of group A .The recurrence rates of group B and C were obviously lower than that of group A .In getting faster improvements of clinical symptom group B and C were better than group C .But the rate of adverse effect of group B was lower than that of group A and C .Conclusion Putting Compound Hippophae Rhamnoides seed oil suppository into vagina and disinfecting vagina and vulva with An ’ erdian Ⅲdisinfectant can effectively treat for bacterial vaginosis .In order to getting symptom relieving faster and controlling the recurrence , this method should be promoted .%目的:观察复方沙棘籽油栓联合安尔碘Ⅲ型消毒剂治疗细菌性阴道病的临床疗效。方法选择临床诊断为细菌性阴道病的患者185例。按照就诊时间次序随机分为A、B、C 3组,A组口服甲

  2. Purification and characterization of water soluble polysaccharide JS1 from Hippophae rhamnoides%沙棘果水溶多糖JS1的分离鉴定

    Institute of Scientific and Technical Information of China (English)

    王桂云; 梁忠岩; 张丽萍; 苗春艳

    1999-01-01

    OBJECTIVE:To study the purification and characterization of water soluble polysaccharide JS1 from Hippophae rahamnoides L.fruits that had been extracted with water.METHODS:JS1 was purified by gel chromatography on DEAE-Sep-hadex A-25.its homogeneity was proved by gel chromatogrophy on Sepharose CL-4B glass fiber paper electrophoresis and specific rotator power.Its stral composition was studied by means of GC,IR,etc.RESULTS:JS1 is a water solube polysaccharide.It is consisted of Ara,Xyl,Gal,Glc with the molarity rate of 1∶6∶12∶4 in turn by means of GC.CONCLUSION:JS1 is a neutral heteropolysaccharide.%目的:从已提取过水溶性多糖后的沙棘果残渣中,进一步用稀碱液(0.1mol.L-1NaOH溶液)提取多糖JS1,并研究其组成性质。方法:利用DEAE-Sephadex A-25柱层析法纯化沙棘果碱提水溶多糖,并采用玻璃纤维纸电泳,Sepharos CL-4B柱层析比旋度等方法证明组分的均一性,同时用气相色谱、红外光谱等方法对其组成进行研究。结果:分离得到JS1水溶性多糖,经气相色谱分析得单糖组分为Ara,Xyl,Gal,Glc,摩尔比依次是1∶6∶12∶4。结论:JS1为中性杂多糖。

  3. Study on Photosynthetic Characteritcs of Hippophae Rhamnoides Linn and Key Impact Factors in Areas of Desertification Combating around Qinghai Lake%青海湖防沙治沙区沙棘光合特性与光响应特征研究

    Institute of Scientific and Technical Information of China (English)

    展秀丽; 严平; 马振宁

    2012-01-01

    为研究青海湖固沙区人工沙棘的光合特性,以青海湖典型的固沙植物沙棘为研究对象,对其光合、蒸腾特性及主要影响因素进行了测定.结果表明,沙棘的光合具有明显的日变化规律,呈双峰型,光合作用速率Pn在12:00左右出现峰值,为21.28 μmol/(m2·s)(文中均以CO2固定量为准),日平均值为13.23 μmol/(m2·s),具有“光午休”现象.蒸腾速率Tr与气孔导度Gs日变化动态也呈双峰型,光合作用有效辐射(Rpa)、空气温度Ta及水汽压强差(pvd)变化规律基本一致,空气湿度(Hr)变化规律则相反;沙棘的Pn,Tr与Rpa,Ta,Gs及pvd呈显著相关,其中,Tr受Ta和pvd影响最为显著;沙棘叶片的水分利用效率(Ewu)的光响应曲线表明,光合有效辐射达到400μmol/(m2·s)时,Ewu最大.对青海湖防沙治沙与生态恢复有重要的参考价值.%The photosynthetic rate, transpiration rate and key impact factors of Hippophae rhamnoides Linn were measured by using the LI-6400 portable photosynthesis system in sand areas around Qinghai Lake. The results showed that the diurnal variation of net photosynthetic rate, transpiration rate and key impact factors were obvious changing rules. And the daily curve of photosynthetic rate and transpiration rate were the double-peak type curves. The midday depression of photosynthesis was obvious. Photosynthetic available radiation (PAR), air temperature ( Ta), and the stomata conductance had positively relationship with the net photosynthetic rate. And the transpiration rate had positively relationship with the vapor pressure deficit. The response curve of water use efficiency ( WUE ) of Hippophae rhamnoides Linn to PAR showed that when PAR was 400 μmol/(m2 · s), WUE arrived at the highest value. The research of the water use efficiency (WUE) of Hippophae rhamnoides Linn will be very important for studying on Hippophae rhamnoides Linn drought resistance mechanism.

  4. Formulation and evaluation of antisebum secretion effects of sea buckthorn w/o emulsion

    OpenAIRE

    Naveed Akhtar; Khan, Barkat A.; Tariq Mahmood; Rashida Parveen; Mughal Qayum; Masood Anwar; Shahiq-uz-zaman,; Muhammad Farooq

    2010-01-01

    Purpose : This study was designed to formulate and evaluate the anti-sebum secretion effects of a topical skin-care cream (w/o emulsion) of sea buckthorn versus its vehicle (Base) as control. Materials and Methods : Concentrated sea buckthorn (H.rhamnoides) fruit extract was entrapped in the inner aqueous phase of w/o emulsion. Base containing no extract and a Formulation containing 1% concentrated extract of H.rhamnoides was formulated. Lemon oil was incorporated to the odor. Both the Base a...

  5. 沙棘果渣总黄酮提取工艺响应面法优化与抗氧化活性研究%Optimizaton of extraction of total flavones from residue of Hippophae rhamnoides L. using response surface methodology and their antioxidant activity

    Institute of Scientific and Technical Information of China (English)

    田景民; 陈贵林

    2014-01-01

    Objective To explore the optimal extraction conditions of total flavonoids from residue of Hippophae rhamnoides L. and evaluate the antioxidant activity. Methods Based on the single-factor tests, the conditions of total flavonoids from residue of Seabuckthorn were optimized by response surface methodology in order to increase the extraction yield of total flavonoids. Meanwhile, the antioxidant activity of total flavonoids was assessed by oxygen radical absorbent capacity (ORAC). Results The optimal extraction conditions of total flavonoids were ethanol concentration 70%, material-liquid ratio 1:25, extraction time 100 min and extraction temperature 80℃. Under the optimal conditions, the maximum extraction yield of total flavonoids was 2.73%. Extracted total flavonoids had a strong antioxidant activity in a dose-dependent manner. Conclusion These investigations will be reference a certain theoretical instruction and application value for the extraction of flavonoids in Seabuckthorn.%目的:优选沙棘果渣总黄酮提取的最佳工艺,并对其抗氧化活性进行评价。方法选取提取时间、温度、料液比、乙醇体积分数为影响因素,在单因素实验结果基础上确定各因素的分析水平,利用响应面中心组合法设计实验方案。并通过体外抗氧化能力指数(ORAC)法研究沙棘果渣总黄酮的抗氧化能力。结果建立了沙棘果渣总黄酮提取的数学模型,最佳提取工艺条件为乙醇体积分数70%、提取温度80℃、提取时间100 min、液料比25:1,沙棘果渣总黄酮具有较强抗氧化活性,与浓度成正相关。结论本研究结果可为沙棘果渣总黄酮工业化提取和应用提供理论指导。

  6. Mechanisms of Action for Total Flavones of Hippophae Rhamnoides and Quercetin on Alkaline Phosphatase Activity in Chicken Osteoblasts%沙棘总黄酮、槲皮素对鸡成骨细胞碱性磷酸酶活性作用机制的体外研究

    Institute of Scientific and Technical Information of China (English)

    刘洪柱; 李垚; 陈鑫; 赵伟; 张丽欣; 欧阳文文; 刘红南

    2011-01-01

    The experiment was conducted to study the effects of total flavones of Hippophae rhamnoides (TFH) and quercetin on alkaline phosphatase (ALP) activity in osteoblasts of arbor acres (AA) broilers' embryo in vitro and its mechanisms of action. Cells were cultured in basal culture medium, basal culture medium supplemented with β-estradiol (E2) , TFH and quercetin for 24 h, respectively; after treated by estrogen receptor (ER) antagonist for 2 h, the cells were cultured for another 24 h in basal culture medium, basal culture medium supplemented with TFH and quercetin, respectively; after treated with extracellular signal-regulated ki-nase (ERK) inhibitor for 2 h, the cells were cultured for another 24 h in basal culture medium, basal culture medium supplemented with TFH and quercetin, respectively; the cells were collected for the analysis of ALP activity. Cells were cultured in basal culture medium supplemented with TFH or quercetin, and collected at six time points (0, 2, 5, 15, 30, 60 min) for the determination of phosphorylated extracellular-regulated kinase (p-ERK) concentration by ELASE. Cells were cultured in basal culture medium, basal culture medium supplemented with E2, TFH or quercetin for 30 min, respectively; after treated by ER antagonist for 15 min, the cells were cultured for 30 min in basal culture medium, basal culture medium supplemented with E2, TFH and quercetin, respectively; cells were collected for the determination of p-ERK concentration by ELASE. The results showed as follows:1) culture medium supplemented with TFH or quercetin significantly increased the ALP activity {P <0.05), and the effects of which were totally prevented by ER antagonist and MEK inhibitor (P<0.05);2)the phosphorylation level of ERK were significantly increased in culture medium supplemented with TFH after 5, 15 min incubation and with quercetin after 2, 5, 15, 30, 60 min incubation (P < 0.05); 3) culture medium supplemented with TFH or quercetin significantly increased

  7. 沙棘叶提取物抗登革病毒的实验研究%Effect of Hippophae rhamnoides Leaf Extract Against Dengue Virus Infection in Human Blood-derived Macrophages

    Institute of Scientific and Technical Information of China (English)

    花圣卓; 李铂岩

    2012-01-01

    Dengue virus occurs as four distinct serotypes,called Dengue 1,2,3,and 4.Symptomaticdengue virus infection ranges from a self limited febrile illness,dengue fever(DF),to a more severedisease,dengue hemorrhagic fever/dengue shock syndrome(DHF/DSS).The anti-Dengue treatmentis severely hampered as no specific therapeutic agents are available.Even present treatmentstrategies for Dengue are more supportive than curative.In the present study anti-dengue activity of Hippoplzae rhamnoides{Seabuckthorn,SBT)leaf extract 4vas evaluated in Dengue virus type-2 infected blood-derived human macrophages as macrophages are the primary target of Denguevirus infection.Infected cells were treated with SBT leaf extract and compared with commerciallyavailable anti-viral drug,Ribavirin.The extract was able to maintain the cell viability of Dengue-infected cells at par with Ribavirin along with the decrease and increase in TNF-[alpha]and IFN-[gamma]respectively.Anti-dengue activity of SBT extract was further determined by the traditionalplaque assay.These observations suggest that the SBT leaf extract has a significant anti-dengueactivity and has the potential for the treatment of Dengue.%登革病毒依抗原性不同,可分为1、2、3、4四个血清型。它除了能导致一定范围内发热的典型登革热外,还能引致更严重的登革出血热和登革休克综合症。由于没有具体有效的治疗药物,目前尚无好的治疗登革热的方法,治疗方案多是支持性的。本实验评估了在2型登革病毒感染的人血源性巨噬细胞中沙棘叶提取物的抗登革病毒活性,选择巨噬细胞的原因是因为巨噬细胞是登革病毒感染的首要目标。受感染的细胞用沙棘叶提取物治疗并与市售的抗病毒药物利巴韦林作比较,研究发现沙棘叶提取物维持登革病毒感染细胞活力的能力几乎等同于利巴韦林。传统的空斑试验进一步确定了沙棘叶提取物的抗登革病毒活性。

  8. Formulation and evaluation of antisebum secretion effects of sea buckthorn w/o emulsion

    Directory of Open Access Journals (Sweden)

    Naveed Akhtar

    2010-01-01

    Full Text Available Purpose : This study was designed to formulate and evaluate the anti-sebum secretion effects of a topical skin-care cream (w/o emulsion of sea buckthorn versus its vehicle (Base as control. Materials and Methods : Concentrated sea buckthorn (H.rhamnoides fruit extract was entrapped in the inner aqueous phase of w/o emulsion. Base containing no extract and a Formulation containing 1% concentrated extract of H.rhamnoides was formulated. Lemon oil was incorporated to the odor. Both the Base and the Formulation were stored at different storage conditions for a period of 4 weeks to predict their stability. Different stability parameters i.e.; physical stability, centrifugation, and pH were monitored at different time intervals. Both the Base and the Formulation were applied to the cheeks of 10 healthy human volunteers (n=10 for a period of 8 weeks. Result : The expected organoleptic stability of creams was achieved from 4 weeks in-vitro study period. Odor disappeared with the passage of time due to volatilization of lemon oil. The pH of the Formulation showed significant ( P = 0.0002 decline due to high concentration of organic acids present in sea buckthorn. Similarly the Formulation showed statistically significant ( P < 0.05 effects on skin sebum secretion. Conclusion : The in vitro results showed a good stability over 4 weeks of observation period of both the Base and Formulation and the Formulation has anti sebum secretion effects over 8 weeks of observation period.

  9. 母株年龄和生长调节剂对俄罗斯大果沙棘嫩枝扦插生根的影响%Effects of donor tree ages and plant growth regulators on the softwood cutting propagation of Hippophae rhamnoides

    Institute of Scientific and Technical Information of China (English)

    杨立学; 王海南; 范晶

    2011-01-01

    ×concentration and age×plant growth regulator concentration were highly significant for percent rate,root number and average root length.The interactive effects of donor tree age×the concentration of plant growth regulators×plant growth regulators were highly significant for rooting rate of H.rhamnoides.

  10. 土壤干旱胁迫下沙棘休眠、萌芽期内源激素变化及外源GA3的调节%Changes of Endogenous Hormone of Sea buckthorn in Dormancy Stage and Sprout Period under Soil Water Stress and Exogenous GA3 Regulation

    Institute of Scientific and Technical Information of China (English)

    李丽霞; 梁宗锁; 魏宇昆; 韩蕊莲

    2001-01-01

    This paper deals with the relationship among water content、endogenous hormone and sprout rate of two years old China sea-buckthorn under soil water stress.The effect of spraying exogenous GA3 in critical time was also researched.The main results show that water content and endogenous GA3 decreased due to water stress ,in the meantime endogenous ABA increased evidently during dormancy stage in winter and sprout period in spring,which lead to the ratio of GA1/3/ABA fell and reached threshold value later.Seedlings under serious water deficit sprouted 25 days later compared to that of control and grew slowly after sprout.Sprout date of seedlings under medium water deficit was postponed about 10 days and growth after sprout was limited slightly.Spraying exogenous 80 mg/L GA3 solution can increase endogenous GA1/3 and GA1/3/ABA of sea-buckthorn with ABA content declining under serious water deficit,so sprout date and growth was promoted effectively.However,spraying exogenous 80 mg/L GA3 solution had little effect on seedlings under appropriate water content and medium water content.%研究了2年生中国沙棘(Hippophae rhamnoids)在土壤干旱胁迫下苗木含水量、内源激素水平与萌芽率关系以及萌芽关键期喷施外源GA3的作用。结果表明:土壤干旱胁迫使冬季休眠与春季萌芽期苗木含水量、内源GA1/3降低,内源ABA明显提高,GA1/3/ABA下降,达到萌动所需的调控阈值的时间延迟,重度干旱下苗木萌芽延迟约25 d,且萌芽后幼枝生长十分缓慢;中度干旱下苗木萌芽延迟10 d,萌芽后生长亦有所抑制。喷施80 mg/L外源GA3溶液可有效提高重度干旱下苗木内源GA1/3,降低ABA含量,使GA1/3/ABA提高,促进苗木提早萌芽及萌芽后生长;在适宜水分及中度干旱下,沙棘苗木外施GA3对萌芽及其生长作用不明显。

  11. SPECTROSCOPIC STUDY OF SEA BUCKTHORN EXTRACTS

    Directory of Open Access Journals (Sweden)

    Carmen Mihaela Topală

    2014-11-01

    Full Text Available The application of sea buckthorn oil is to incorporate the oil into foodstuffs such as milk, yoghurt, cheese, butter, juice and snacks which represents new opportunities for food manufacturers, food supplements and nutraceuticals providing nutritional supports. The FTIR spectroscopy is a powerful technique for assessing food production and studied materials provides fundamental information on the behavior of the spectral metabolites and bio product. The extracts were studied from two varieties of sea buckthorn oil Pitesti I and II. Oil obtained from peel and seeds by the Soxhlet extraction with hexane solvent and CO2 supercriticalwas analyzed by FTIR spectroscopy. The concentration of fatty acids in oil extracted from seeds and peels was similar in both extraction techniques.

  12. Acute and sub acute toxicity and efficacy studies of Hippophae rhamnoides based herbal antioxidant supplement

    Directory of Open Access Journals (Sweden)

    Rashid Ali

    2012-01-01

    Conclusion: The data obtained indicate no toxicity of this antioxidant supplement up to the highest dose studied. Efficacy in terms of increased bioavailability of vitamin A and C in human volunteers indicates the clinical usefulness of the supplement.

  13. Attributes of Seabuckthorn (Hippophae rhamnoides L. to Meet Nutritional Requirements in High Altitude.

    Directory of Open Access Journals (Sweden)

    Tsering Stobdan

    2010-03-01

    Full Text Available The diet of humans living in different geographical and climatic regions of the earth varies greatly in both quantity and composition of foods. Evidence is accumulating that indicates that there is a high risk of malnutrition at high altitude because of the usual lack of fresh food and environmental factors. Lack of nutritious diet in the difficult terrain is a potential stressor that elicits oxidative stress. The excretion of minerals from the body is higher in high altitude condition. The altered nutritional requirement can be met to a large extend by regular consumption of locally grown fruits and vegetables. Results of analysis of Seabuckthorn growing in Leh valley of Trans-Himalaya showed the presence of high content of multivitamins including vitamin C (275 mg/100g, vitamin A (432.4 IU/100g, vitamin E (3.54 mg/100g, Riboflavin (1.45 mg/100g, Niacin (68.4 mg/100g, Pantothenic acid (0.85 mcg/100g, vitamin B-6 (1.12 mg/100g, and vitamin B-2 (5.4 mcg/100g. Similarly, mineral elements composition revealed high amount of minerals including potassium (647.2 mg/l, calcium (176.6 mg/l, iron (30.9 mg/l, magnesium (22.5 mg/l, phosphorous (84.2 mg/l, sodium (414.2 mg/l, zinc (1.4 mg/l, copper (0.7 mg/l, manganese (1.06 mg/l and selenium (0.53 mg/l.Defence Science Journal, 2010, 60(2, pp.226-230, DOI:http://dx.doi.org/10.14429/dsj.60.344

  14. Optimization of de novo short read assembly of seabuckthorn (Hippophae rhamnoides L. transcriptome.

    Directory of Open Access Journals (Sweden)

    Rajesh Ghangal

    Full Text Available Seabuckthorn (Hippophaerhamnoides L. is known for its medicinal, nutritional and environmental importance since ancient times. However, very limited efforts have been made to characterize the genome and transcriptome of this wonder plant. Here, we report the use of next generation massive parallel sequencing technology (Illumina platform and de novo assembly to gain a comprehensive view of the seabuckthorn transcriptome. We assembled 86,253,874 high quality short reads using six assembly tools. At our hand, assembly of non-redundant short reads following a two-step procedure was found to be the best considering various assembly quality parameters. Initially, ABySS tool was used following an additive k-mer approach. The assembled transcripts were subsequently subjected to TGICL suite. Finally, de novo short read assembly yielded 88,297 transcripts (> 100 bp, representing about 53 Mb of seabuckthorn transcriptome. The average length of transcripts was 610 bp, N50 length 1198 BP and 91% of the short reads uniquely mapped back to seabuckthorn transcriptome. A total of 41,340 (46.8% transcripts showed significant similarity with sequences present in nr protein databases of NCBI (E-value < 1E-06. We also screened the assembled transcripts for the presence of transcription factors and simple sequence repeats. Our strategy involving the use of short read assembler (ABySS followed by TGICL will be useful for the researchers working with a non-model organism's transcriptome in terms of saving time and reducing complexity in data management. The seabuckthorn transcriptome data generated here provide a valuable resource for gene discovery and development of functional molecular markers.

  15. Several Lipophilic Components of Five Elite Genotypes of Romanian Seabuckthorn (Hippophae rhamnoides subs. carpatica

    Directory of Open Access Journals (Sweden)

    Liviu Adrian VESCAN

    2010-09-01

    Full Text Available Seabuckthorn is a spinescent, deciduous and dioecious berry-producing shrub, with a high economical and ecological potential. It is frequently used as a pioneer species in anthropic and eroded soils due to its low pedoclimatic demands, strong rooting system and ability to fix atmospheric nitrogen. Seabuckthorn berries, leaves and bark have a high content of nutritive and active substances which promote this species for use in both food and medical industries. One of the most requested therapeutical products on the market is the seabuckthorn oil, extracted from both pulp and seeds. Two important parameters in analyzing seabuckthorn oil quality are fatty acids and tocopherols. In Transylvania region most of seabuckthorn orchards are established with local, low productive and less uniform planting material, randomly collected from wild flora. In order to assess the opportunity of introducing new seabuckthorn varieties in Transylvania, a selection process was initiated. In this context, five elites were selected from wild populations in the Danube Delta, using biometrical criteria. They were later compared to a representative individual from a local population and to a number of homologated cultivars, with respect to morphology and some lipophilic components (oil content, fatty acids and tocopherols. For both pulp and seeds, total lipids were extracted using a modified Folch method. Fatty acids were analyzed by gas chromatography using flame ionization detection. Tocopherols were analysed using a Shimadzu VP Series liquid chromatograph with a fluorescence detector FR-10 AXL.

  16. In silico -- based combinatorial pharmacophore modelling and docking studies of GSK-3 and GK inhibitors of Hippophae

    Indian Academy of Sciences (India)

    Sushil Kumar Middha; Arvind Kumar Goyal; Syed Faizan; Nethramurthy Sanghmitra; Bharat Chandra Asistha; Talambedu Usha

    2013-11-01

    Type 2 diabetes is an inevitably progressive disease, with irreversible cell failure. Glycogen synthase kinase and Glukokinase, two important enzymes with diverse biological actions in carbohydrate metabolism, are promising targets for developing novel antidiabetic drugs. A combinatorial structure-based molecular docking and pharmacophore modelling study was performed with the compounds of Hippophae salicifolia and H. rhamnoides as inhibitors. Docking with Discovery Studio 3.5 revealed that two compounds from H. salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 receptor and play a role in its inhibition; whereas in the case of Glucokinase, only one compound from both the plants, i.e. vitamin C, had good binding characteristics capable of activation. The results help to understand the type of interactions that occur between the ligands and the receptors. Toxicity predictions revealed that none of the compounds had hepatotoxic effects and had good absorption as well as solubility characteristics. The compounds did not possess plasma protein-binding, crossing blood–brain barrier ability. Further, in vivo and in vitro studies need to be performed to prove that these compounds can be used effectively as antidiabetic drugs.

  17. USE OF SEA BUCKTHORN FRUITS IN THE PASTRY MANUFACTURING

    OpenAIRE

    RODICA A. STURZA; ALIONA A. GHENDOV-MOŞANU; OLGA I. DESEATNICOV; NATALIA F. SUHODOL

    2016-01-01

    Different researches on optimizing the shelf life of pastries (gingerbread, sponge cakes) that are produced by adding sea buckthorn flour (2 - 4 % by weight of the flour used) are presented in this study. This study shows the impact of biologically active substances on structural and mechanical, physicochemical, microbiological properties as well as the antioxidant activity of products under the conditions of in vitro gastric digestion. It has been demonstrated that the sea buckthorn flour in...

  18. USE OF SEA BUCKTHORN FRUITS IN THE PASTRY MANUFACTURING

    Directory of Open Access Journals (Sweden)

    RODICA A. STURZA

    2016-04-01

    Full Text Available Different researches on optimizing the shelf life of pastries (gingerbread, sponge cakes that are produced by adding sea buckthorn flour (2 - 4 % by weight of the flour used are presented in this study. This study shows the impact of biologically active substances on structural and mechanical, physicochemical, microbiological properties as well as the antioxidant activity of products under the conditions of in vitro gastric digestion. It has been demonstrated that the sea buckthorn flour increases the porosity of pastries, reduces the wet gluten amount and this contributes to moisture loss. The organoleptic assessment indicates that the addition of 2 % sea buckthorn flour improves the appearance, the color and the consistency of pastries. Microbiological analysis showed that samples with added sea buckthorn flour exhibit microbiological stability due to the sea buckthorn chemical composition. The antiradical activity DPPH• in conditions of in vitro gastric digestion of the samples with added sea buckthorn flour increases in a positive way, indicating a clearly positive effect on health.

  19. Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert

    OpenAIRE

    Dhar, Priyanka; BAJPAI, Prabodh Kumar; Tayade, Amol Bapurao; CHAURASIA, Om Prakash; Srivastava, Ravi Bihari; Singh, Shashi Bala

    2013-01-01

    Background Himalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca) and roseroot (Rhodiola imbricata) from trans-Himalaya. Methods The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-...

  20. The Effects of Total Flavones of Hippophae Rhamnoides L. on Cardiac function and Hemodynamics of Normal Subjects

    Institute of Scientific and Technical Information of China (English)

    冯养正; LI Yi; LIAO Zhao-jun

    2006-01-01

    Healthy volunteers were randomly divided into TFH group(22 subjects) and placebo control group (20 subjects). We used double-blind method. The cardiac performance and hemodynamics of the subjects before and after administration of TFH or placebo were determined by Admittance and STI methods, Oral TFH in a dose of 10 mg could significantly decrease the PEP/LVET, ICT/LVET and ICT of the mormal subjects(P<0.05). Oral TFH could increase SV(P<0.05). CO, CI, SWI, CWI and LI also tended to increase. Oral TFH also markedly reduced TPR (P<0.05) and increasedc (P<0.05). After administration of TFH, HR and HP did not significantly change(P> 0.05). After administration of placebo, the parameters observed did not significantly change(P>0.05). The above results show that TFH can strengthen myocardial contractility and pump function of the heart, reduce total peripheral vascular resistance and increase vascular clasticity in normal subjects.

  1. Whitening efficacy of plant extracts including Hippophae rhamnoides and Cassia fistula extracts on the skin of Asian patients with melasma

    OpenAIRE

    Khan, Barkat Ali; Akhtar, Naveed; Irshad HUSSAIN; Abbas, Khwaja Asad; Rasul, Akhtar

    2013-01-01

    Introduction Melasma/hyperpigmentation and solar damage of the skin remains a difficult problem to treat. Various types of whitening agents are used to treat hyperpigmentation. A change has been observed recently to use plant extracts as skin whitening agents. Aim To compare the effectiveness of emulsion formulations containing plant extracts that include catechins/polyphenols and placebo without plant extracts, on patients with melasma. Material and methods Two groups of 25 patients each (ag...

  2. Effect of sea buckthorn and vitamin E on growth performance of Acipenser stellatus (Pallas 1771) juveniles

    OpenAIRE

    Oana Georgiana Dorojan; Victor Cristea; Mirela Crețu; Lorena Dediu; Iulia Rodica Grecu; Săndița Plăcintă

    2015-01-01

    Addition of natural additives (prebiotics, probiotics, phytobiotics) in fish feed is a benefic method because it leads to improved growth performance of fish reared in intensive production systems. Sea buckthorn is one of the most commonly phytobiotics used in aquaculture because of its antioxidant, antimicrobial, immunostimulant, anti-stress, cytoprotective, liver and tissue regeneration properties. Sea buckthorn berries are rich in vitamins (B, C, E and K), flavonoids, carotenoids, tocopher...

  3. Efficacy of Control Measures for European Buckthorn ( Rhamnus cathartica L.) in Saskatchewan

    Science.gov (United States)

    Delanoy, Luc; Archibold, O. W.

    2007-10-01

    Introduced to Saskatchewan in the 1930s as a potential shelterbelt species, European buckthorn is now a prominent understory shrub in riparian woodland and shrub communities around Saskatoon. Locally, the Meewasin Valley Authority (MVA) is actively controlling buckthorn as part of its mandate to conserve natural heritage resources of the South Saskatchewan River Valley, with the goal of restoring the natural biodiversity of remnant patches of native vegetation. European buckthorn is normally dioecious, and MVA has chosen to treat only fruiting stems in an attempt to limit seed production. Two control techniques have been used. In one treatment, glyphosate was applied to stems after cutting; alternatively Garlon 4 Dow AgroSciences herbicide (active ingredient triclopyr) was applied as a chemical girdle directly to the stems using a streamline basal bark spray method. To date, more than 347,000 fruiting stems of buckthorn have been treated. Results indicate good initial progress in limiting seed production in dense buckthorn sites, but at a high cost. Although seed eradication is not a practical short-term goal for the Saskatoon buckthorn population, chemical girdling can substantially and strategically reduce seed and effectively limit spread. Field-tested strategies to reduce costs and improve efficiencies are discussed.

  4. Achievement of a Fortifying Product based on Apples, Quinces and Sea Buckthorn

    Directory of Open Access Journals (Sweden)

    Adriana VOICU

    2009-06-01

    Full Text Available The aim of the research was to achieve a fortifying product Apple, Quince and Sea Buckthorn Nectar rich in antioxidants (vitamin C, ?-carotene and dietary fibers (cellulose, pectic substances from fruit. In the experiments were used fruit (apples, quinces and sea buckthorn with a high content of these nutrients. The antioxidants are chemical substances that inhibit, decompose or prevent forming of free radicals or other substances that induce the cancerogenesis. Also, dietary fibers are another group of natural compounds with important role in prevention of the digestive diseases. In the experiments were used biochemical methods for analysis of fruit and processed products (determination of soluble dry substance, glucides, proteins, lipids, mineral elements, total acidity, vitamin C, ?-carotene, cellulose, pectic substances etc.. Also were performed microbiological analysis and sensorial analysis for processed products.In order to obtain the product Apple, Quince and Sea Buckthorn Nectar, four variants of experiments were carried out at the laboratory level. Following the sensorial and biochemical analyses, the optimum variant of the product Apple, Quince and Sea Buckthorn Nectar was selected, namely the variant V3 in which the ratio apple puree and quince puree was 1:1 and the percentage of added sea buckthorn juice was of 3%.

  5. Studies on chemical composition of leaves of H.rhamnoides L.Subsp.Sinensis Rousi( Ⅲ )%中国沙棘叶化学成分的研究(Ⅲ)

    Institute of Scientific and Technical Information of China (English)

    杨亮; 叶蓁; 李教社; 宋玉乔; 路平; 杨银京; 卢萍

    2004-01-01

    为了研究中国沙棘叶化学成分,采用硅胶柱层析、Sephadex LH-20柱层析进行分离,并通过理化常数测定和波谱技术进行结构鉴定.结果从沙棘Hippophae rhamnoides L.的乙酸乙酯提取物中分离并鉴定了2个黄酮化合物:山萘素-3-β-D-(6"-对羟基桂皮酰基)葡萄糖甙(kaempf-erol 3-β-D-p-coumaroyD-glucopyranoside,Ⅰ),异鼠李素-7-0-鼠李糖-3-0-葡萄糖甙(cisorhamnetin-7-0-rhamnose-3-0-glucoside,Ⅱ).结果表明山萘素-3-β-D-(6"-对羟基桂皮酰基)葡萄糖甙为首次从该属植物中得到.

  6. Study on saponification technology of Hippophae rhamnoides L.seed oil%沙棘籽油皂化工艺的研究

    Institute of Scientific and Technical Information of China (English)

    王思寒; 石品艳; 冯凤琴

    2009-01-01

    以沙棘籽油为原料,研究了3种因素,即皂化时间、皂化温度和KOH-乙醇溶液浓度对沙棘籽油皂化效果的影响,结果表明,较好的皂化条件为皂化时间60min、皂化温度70%、KOH-乙醇溶液浓度为1.0mol/L.在此条件下脂肪酸得率为91.08%,皂化产物油酸含量为17.8%,亚油酸含量为27.2%,亚麻酸含量为24.9%.

  7. Mechanism of the Promotion of Drought Resistance of Hippophae rhamnoides with Arbuscular Mycorrhizal Fungi%AMF提高沙棘抗旱性的研究

    Institute of Scientific and Technical Information of China (English)

    唐明; 薛萐; 任嘉红; 胡景江; 刘建朝

    2003-01-01

    为揭示丛枝菌根真菌(AMF)提高沙棘抗旱性的机制,对与沙棘菌根化苗木的抗旱性相关生理生化指标进行了研究.结果表明干旱胁迫条件下,随着AMF侵染率的增加,叶片的超氧化物歧化酶(SOD)活性增加,膜脂过氧化产物丙二醛(MDA)含量和细胞质膜相对透性(RP)降低.AMF侵染率较高的植物体内SOD活性维持较高水平,可有效清除宿主植物体内因干旱胁迫而积累的超氧自由基,降低MDA含量和细胞质膜相对透性,减轻膜脂过氧化造成的伤害程度,增强了植物的抗旱性.

  8. Study on the Karyotype of Three Subspecies from Hippophae rhamnoides%3种沙棘染色体核型研究

    Institute of Scientific and Technical Information of China (English)

    刘洪章; 生利霞

    2011-01-01

    The karyotypes of China seabuckthom, Russia seabuckthom and Mongolia seabuckthorn were analysed in this study. The results showed that three seabuckthorn all have 24 chromosomes and all are diploid, metacentric chromosome. China seabuckthorn and Mongolia seabuckthorn have satellites at 4th chromosome, Russia seabuckthorn at 2nd chromosome. The karyotype are all 2A and symmetrical.%对生长在同一生境的中国沙棘、蒙古沙棘和俄罗斯沙棘的核型进行了比较研究.结果表明:3种沙棘的染色体数目均为2n=24,都由中部着丝粒染色体组成.其中,中国沙棘、蒙古沙棘在第4对染色体上有随体,俄罗斯沙棘在第2对染色体上有随体.3种沙棘的核型类型都是2A型,比较对称.

  9. Oat Hulls and Sea Buckthorn Pomace – a Potential Source of Antioxidants for Hempseed Oil

    OpenAIRE

    Zēberga, S; Strēle, M; Vojevodova, A; Mieriņa, I; Jure, M

    2014-01-01

    Hempseed oil, as well as other oils containing polyunsaturated fatty acids, is subjected to oxidation processes caused by air, heat or light. Usually these processes are suppressed by addition of various synthetic phenol type antioxidants. The aim of current research was to increase oxidative stability of vegetable oils with natural antioxidants. Herein, we demonstrate that the oxidative stability can be increased with natural antioxidants present in oat hulls and sea buckthorn pomace – by-pr...

  10. Sea buckthorn bud extract displays activity against cell-cultured Influenza virus

    OpenAIRE

    TORELLI, A.; GIANCHECCHI, E.; PICCIRELLA, S.; MANENTI, A.; Piccini, G.; LLORENTE PASTOR, E.; CANOVI, B.; MONTOMOLI, E.

    2015-01-01

    Summary Introduction. Vaccines and antiviral drugs are the most widely used methods of preventing or treating Influenza virus infection. The role of sea buckthorn (SBT) bud dry extract as a natural antiviral drug against Influenza was investigated. Methods. Influenza virus was cultured in the MDCK cell line, with or without SBT bud extract, and virus growth was assessed by HA and TCID50 virus titration in terms of cytopathic effect on cells. Several concentrations of extract were tested, the ...

  11. Technology of drinks based on water extracts of rosehip, sea buckthorn and viburnum

    OpenAIRE

    Капліна, Тетяна Вікторівна; Миронов, Денис Анатольевич

    2014-01-01

    The results of developing the technology of drinks, fruit drinks and fizzes, made of plant extracts of rosehip, sea buckthorn and viburnum, previously processed in the vortex layer of ferromagnetic particles are presented in the paper. Plant extracts were prepared by the traditional infusion at a constant temperature. Processing in the vortex layer of ferromagnetic particles allows to reduce the infusion time by 4-6 times in comparison with the industrial extraction technologies.Based on the ...

  12. Effect of sea buckthorn and vitamin E on growth performance of Acipenser stellatus (Pallas 1771 juveniles

    Directory of Open Access Journals (Sweden)

    Oana Georgiana Dorojan

    2015-05-01

    Full Text Available Addition of natural additives (prebiotics, probiotics, phytobiotics in fish feed is a benefic method because it leads to improved growth performance of fish reared in intensive production systems. Sea buckthorn is one of the most commonly phytobiotics used in aquaculture because of its antioxidant, antimicrobial, immunostimulant, anti-stress, cytoprotective, liver and tissue regeneration properties. Sea buckthorn berries are rich in vitamins (B, C, E and K, flavonoids, carotenoids, tocopherols and many volatile compounds. In this context the aim of this study was to evaluate the effects of dietary vitamin E (500 mg/kg diet and sea buckthorn on growth performance and survival of the A. stellatus juveniles reared in a recirculating aquaculture system. The experiment took place in the recirculating pilot system of “Dunărea de Jos” University of Galați. In order to emphasize the influence of the above mentioned immunostimulants on sevruga fingerlings with different genetic background, four experimental groups from different genitors (V1: ♀2 Danube x ♂1 Aquaculture, V2: ♀1 Danube x ♂1 Danube, V3: ♀1 Danube x ♂2 Aquaculture and V4: ♀2 Danube x ♂2 Danube have been used. At the end of the experiment no significant differences (p>0.05 were observed in terms of final mean weight, individual growth rate, SGR, FCR, PER, between the sevruga fingerlings obtained from wild genitors, comparing with those obtained from aquaculture genitors.

  13. Effect of modified atmosphere packaging (map) on the quality of sea buckthorn berry fruits during postharvest storage

    Science.gov (United States)

    The effect of modified atmosphere packaging (MAP) on the quality of the berry fruits of sea buckthorn (SBT) during refrigerated storage was investigated. SBT berries were packaged in 160 and 525 oxygen transmission rate (OTR) films or in vented clamshell containers (air control) and stored at 10C fo...

  14. Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses.

    Science.gov (United States)

    Dorhoi, A; Dobrean, V; Zăhan, M; Virag, P

    2006-05-01

    Standardized ethanol extracts of Allium sativum (garlic), Glycyrrhiza glabra (licorice), Plantago major (plantain) and Hippophae rhamnoides (sea buckthorn) were assessed for their effects on cellular immunity in laying hens. Birds (n = 25) had blood samples taken and both specific and non-specific immune cell responsiveness were evaluated by a leukocyte proliferation assay, carbon clearance test and SRBC phagocytosis in monocyte-derived macrophage cultures. Licorice and sea buckthorn (50 microg/mL) clearly enhanced the macrophage membrane function (p effects on circulating phagocytes were revealed for plantain and sea buckthorn, while garlic at 200 microg/mL impaired the phagocytic capacity of blood cells. None of the tested extracts showed mitogenic properties, but high concentrations of sea buckthorn (400 microg/mL) inhibited leukocyte proliferation. Small concentrations (20 microg/mL) of licorice proved the co-mitogenic potential for both T and B avian lymphocytes (p < 0.05). Certain extracts definitely enhanced the fowl innate and/or specific cell immunity and may therefore improve host resistance in poultry. Considering the chicken as an important non-mammalian model that also serves as an available laboratory approach for some human diseases, herbs exerting immunomodulatory properties may find relevant clinical applications. PMID:16619362

  15. Investigation on the antidepressant effect of sea buckthorn seed oil through the GC-MS-based metabolomics approach coupled with multivariate analysis.

    Science.gov (United States)

    Tian, Jun-sheng; Liu, Cai-chun; Xiang, Huan; Zheng, Xiao-fen; Peng, Guo-jiang; Zhang, Xiang; Du, Guan-hua; Qin, Xue-mei

    2015-11-01

    Depression is one of the prevalent and serious mental disorders and the number of depressed patients has been on the rise globally during the recent decades. Sea buckthorn seed oil from traditional Chinese medicine (TCM) is edible and has been widely used for treatment of different diseases for a long time. However, there are few published reports on the antidepressant effect of sea buckthorn seed oil. With the objective of finding potential biomarkers of the therapeutic response of sea buckthorn seed oil in chronic unpredictable mild stress (CUMS) rats, urine metabolomics based on gas chromatography-mass spectrometry (GC-MS) coupled with multivariate analysis was applied. In this study, we discovered a higher level of pimelic acid as well as palmitic acid and a lower level of suberic acid, citrate, phthalic acid, cinnamic acid and Sumiki's acid in urine of rats exposed to CUMS procedures after sea buckthorn seed oil was administered. These changes of metabolites are involved in energy metabolism, fatty acid metabolism and other metabolic pathways as well as in the synthesis of neurotransmitters and it is helpful to facilitate the efficacy evaluation and mechanism elucidating the effect of sea buckthorn seed oil for depression management.

  16. Above-ground biomass models for Seabuckthorn (Hippophae salicifolia) in Mustang District, Nepal

    DEFF Research Database (Denmark)

    Rajchal, Rajesh; Meilby, Henrik

    2013-01-01

    Seabuckthorn (Hippophae salicifolia D. Don.), an important multi-purpose tree, is found at altitudes of 2000–3600 m amsl in Nepal, but so far no models have been developed for estimating the biomass of this species, thus hampering resource assessment and management planning. Hence, the objective...

  17. Identification of urinary biomarkers after consumption of sea buckthorn and strawberry, by untargeted LC-MS metabolomics

    DEFF Research Database (Denmark)

    Cuparencu, Catalina Sinziana; Andersen, Maj-Britt Schmidt; Gürdeniz, Gözde;

    2016-01-01

    Berries may improve health; however, accurate assessment of berry intake is still problematic. The discovery of objective biomarkers for intake of berries is therefore important in assessing both intake and compliance. We aimed to identify urinary exposure markers of two very different berries...... in very low concentrations and therefore not identified. Nine and 11 markers were (tentatively) identified for strawberry and sea buckthorn, respectively, the most specific being conjugates of aroma compounds and coloring flavonoids. Metabolites reflecting the step-wise aromatization of quinic to hippuric...

  18. Vitamin E and Hippophea rhamnoides L. extract reduce nicotine-induced oxidative stress in rat heart.

    Science.gov (United States)

    Gumustekin, Kenan; Taysi, Seyithan; Alp, Hamit Hakan; Aktas, Omer; Oztasan, Nuray; Akcay, Fatih; Suleyman, Halis; Akar, Sedat; Dane, Senol; Gul, Mustafa

    2010-06-01

    The effects of vitamin E and Hippophea rhamnoides L. extract (HRe-1) on nicotine-induced oxidative stress in rat heart were investigated. There were eight rats per group and supplementation period was 3 weeks. The groups were: nicotine [0.5 mg kg(-1)day(-1), intraperitoneal (i.p.)]; nicotine plus vitamin E [75 mg kg(-1)day(-1), intragastric (i.g.)]; nicotine plus HRe-1 (250 mg kg(-1)day(-1), i.g.); and the control group (receiving only vehicles). Nicotine increased the malondialdehyde level, which was prevented by both vitamin E and HRe-1. Glutathione peroxidase (GPx) activity in nicotine plus vitamin E supplemented group was higher than the others. Glutathione S-transferase (GST) activity in nicotine plus HRe-1 supplemented group was increased compared with the control group. Catalase activity was higher in nicotine group compared with others. GPx activity in nicotine plus vitamin E supplemented group was elevated compared with the others. Total and non-enzymatic superoxide scavenger activities in nicotine plus vitamin E supplemented group were lower than nicotine plus HRe-1 supplemented group. Superoxide dismutase (SOD) activity was higher in nicotine plus HRe-1 supplemented group compared with others. Glutathione reductase activity and nitric oxide level were not affected. Increased SOD and GST activities might have taken part in the prevention of nicotine-induced oxidative stress in HRe-1 supplemented group in rat heart. Flavonols such as quercetin, and isorahmnetin, tocopherols such as alpha-tocopherol and beta-tocopherol and carotenoids such as alpha-carotene and beta-carotene, reported to be present in H. rhamnoides L. extracts may be responsible for the antioxidant effects of this plant extract. PMID:20517898

  19. Study on the impact of fermentation process to sea buckthorn icewine quality%发酵条件对沙棘冰酒品质的影响

    Institute of Scientific and Technical Information of China (English)

    李树志; 鲁长征; 山永凯; 刘洪智; 任蓓蕾

    2009-01-01

    The sea buckthorn juice was used as raw material, and the fermentation process parameters of ice sea buckthorn wine were studied, including color, organization, smell and taste. Through orthogonal experiment, the best fermentation process parameters of ice sea buckthorn wine could be gotten:fermentation temperature 24 ℃, yeast inoculum 3% (V/V, 106/mL), pH 3. 6, fermentation time 20 d. Under these conditions, the ice sea buckthorn wine had better color, organization, smell, taste and suitable for large-scale production.%以沙棘汁为原料研究发酵工艺中各参数对沙棘冰酒品质的影响.通过L9(34)正交试验,得出制备沙棘冰酒的最佳发酵工艺参数为:发酵温度24℃,酵母接种量3%(V/V,106个/mL),PH 3.6,发酵时间20 d.在此条件下,沙棘冰酒的色泽、组织状态、滋味、口感均较理想,适合企业规模化生产.

  20. Extraction of isorhamnetin from marc of Hippophae rhamnoides L.using ultrasound-assisted method%沙棘果渣中异鼠李素超声辅助提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    胡丹; 何珺; 杨佳年

    2014-01-01

    采用超声波法提取沙棘果渣中异鼠李素.首先采用单因素对超声频率、提取时间、次数、提取温度、提取料液比及提取溶剂进行筛选,再对筛选提取条件进行正交验证试验.确定了该方法提取沙棘果渣中异鼠李素的参数,即50℃,超声频率40 kHz的条件下用V乙酸乙酯∶V95%乙醇为4∶6的提取液超声2次,每次1h,料液比1∶25,所得的异鼠李素含量可达5.09 mg·g-1.

  1. 复合溶剂提取沙棘籽油的工艺条件研究%Extraction of seabuckthorn seed oil with mixed solvent from Hippophae rhamnoides seed

    Institute of Scientific and Technical Information of China (English)

    张丽霞; 康健; 吴桐

    2011-01-01

    采用响应面优化了正己烷-丙酮复合溶剂浸提沙棘籽油的提取条件,并对沙棘籽油的理化性质进行分析.结果表明:最优条件为料液比1∶14、粉碎度35.00目、正己烷:丙酮为6∶4、浸提温度44.00℃、浸提时间1.84h,在该条件下沙棘籽油提取率为13.788%.沙棘籽油的碘价为156.30mg/100g,酸价为8.80mg KOH/g,皂化值为186.30mg KOH/g,水分及挥发物0.22%,过氧化值为9.21meq/kg,不皂化物含量为1.89%.%Extraction conditions of seabuckthom seed oil with mixed solvents were optimized with response surface methods (RSM) and physiochemical properties of crude oil were analysed. The optimum conditions were as follows: ratios of solid to liquid 1:14, grinding size 35.00 mesh,mixed solvent as nhexane to acetone 6:4, extraction temperature 44.00℃ and extraction time 1.84h. The extraction rate of seabuckthorn seed oil was 13.788% under the optimum conditions. The iodine value, acid value ( AV ) and saponification value of seabuckthorn seed oil were 156. 30mg/100g, 8.80g KOH/g and 186. 30mg KOH/g, respectively. And the water volatiles, peroxide value and unsaponifiable value were 0.22%, 9.21meq/kg, and 1.89%, respectively.

  2. 醋柳黄酮和银杏叶提取物含量测定的差异性%Study on its variability of determination method of Total Flavones of total flavones of Hippophae rhamnoides and Ginko Biloba Extract

    Institute of Scientific and Technical Information of China (English)

    孙华; 王玮; 凌春生; 周绍华; 李燕真

    2008-01-01

    目的:分别以槲皮素、异鼠李素为对照品测定醋柳黄酮与银杏叶提取物中总黄酮含量,研究是否可以用槲皮素代替异鼠李素作对照品.方法:采用分光光度法测定醋柳黄酮与银杏叶提取物中总黄酮含量.结果:以槲皮素折合,醋柳黄酮中总黄酮含量为8.90%,银杏提取物中总黄酮为4.41%;以异鼠李素折合,醋柳黄酮中总黄酮含量为16.80%,银杏提取物中总黄酮含量为8.25%.结论:分别以槲皮素、异鼠李素为对照品测得同一样品,总黄酮含量差异很大,不能简单用槲皮素代替异鼠李素进行样品总黄酮的含量测定.

  3. 国外生态经济树种沙棘引种驯化及早期评价%Introduction and Domestication as Well as Early Evaluation of Hippophae rhamnoides of Foreign Ecological and Economic Species

    Institute of Scientific and Technical Information of China (English)

    崔云英; 兰士波; 温爱亭

    2011-01-01

    为丰富我国高寒地区生态经济林树种遗传多样性,促进林业生态体系和产业体系建设,遵循供种区与引种区环境条件相似的原则,根据引种区气候、土壤、植被分布、地形地貌及地理位置等主要环境条件,确定与之相适宜俄罗斯为供种区。以6个俄罗斯大果沙棘优良品种为引种对象,在引种驯化及区域试验的基础上,综合分析与评价子代生态适应性、生长表现及果实品质,确定YSH和HJN为高寒地区引种栽培的适宜品种,且品种内家系间数量性状变异较小,生长稳定,具广泛推广应用价值。%In order to enrich the resources ot PLcological and Economlc iorest species in the cold area,develop variety of the creature and accelerate the construction of ecological and industrial system, Following resemble principle between the environmental conditions of seed zone and introduction area, The areas introducing fine varieties in the cold area confirm to regard the suitable the Russia as the area of origin ac- cording to its own climate, soil, vegetation distribution, landform and the general configuration of the earth's surface, geographical position and the other main environmental situations. The Russia's Seabuckthorn improved varieties was employed to analyze and evaluate the adaptability, growth performance of seeding and fruit quality of foreign ecological and economic species was farther studied based on regionalization test, introduction and domestication. It was held preliminarily that two varieties of YSH, HJN grew well and the growth of quantitative character varied slightly within families which remained stable, and possess extensive generalization and utilization value.

  4. Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H+) donation capability: Ex vivo and in vivo studies

    OpenAIRE

    Mainak Chakraborty; Indrajit Karmakar; Sagnik Haldar; Avratanu Das; Asis Bala; Pallab Kanti Haldar

    2016-01-01

    Introduction: The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages. Material and Methods: In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H+ donating capacity. Mouse erythrocytes' hemolysis and peritoneal macrophages' DNA damage were determined spectrophotometrically. In vivo a...

  5. Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H+) donation capability: Ex vivo and in vivo studies

    OpenAIRE

    Chakraborty, Mainak; Karmakar, Indrajit; Haldar, Sagnik; Das, Avratanu; Bala, Asis; Haldar, Pallab Kanti

    2016-01-01

    Introduction: The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages. Material and Methods: In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H+ donating capacity. Mouse erythrocytes’ hemolysis and peritoneal macrophages’ DNA damage were determined spectrophotometrically. In vivo a...

  6. 沙棘果酒酿造中优良酵母菌的筛选及鉴定%Study on screening and identification of excellent yeast strains for producing sea-buckthorn wine

    Institute of Scientific and Technical Information of China (English)

    隆文娟; 王捷; 姚玉军; 邬晓萍; 陶文沂

    2011-01-01

    Nine yeast strains were isolated from mature sea-buckthorn peel. With plenty of testing experiments such as the gas formation ability, the alcohol yield and the fermentation capacity, as well as the sensory evaluation of sea-buckthorn wine fermented by different yeast strains, strain Rl was screened. Strain Rl was a very good strain for making sea-buckthorn wine from sea-buckthorn juice with high acidity as it starting fermentation fast with strong ability. So as the quality of sea-buckthorn wine produced by Rl was excellent with a clear and transparent wine body, a wine-red color and luster, and a typical model taste of the sea-buckthorn wine. Strain Rl was suitable for producing vinegar besides sea-buckthorn wine. By molecular biological method strain Rl was identified as a new strain of Saccharomyces cerevisiae.%从成熟的沙棘果皮中筛选得到9株酵母茵,通过产气性能、产酒精能力和发酵力测试试验及对发酵所得的沙棘果酒的感官评价,选出R1为最佳的沙棘果酒酿酒用茵株.该茵株适合在酸度较高的沙棘果汁中进行正常的酒精发酵,起酵快、发酵能力强,发酵所得沙棘果酒品质优,而且酒体澄清透明、色泽红润,具有沙棘果酒的典型风味,适用于果酒生产以及下一步的沙棘果醋生产试验.经过分子生物学鉴定,确定R1为Saccharomyces cerevisiae的一个新菌株.

  7. 沙棘叶黄酮提取物的稳定性研究%Stability of Flavonoids Extracts of Sea Buckthorn Leaves

    Institute of Scientific and Technical Information of China (English)

    殷丽君; 冯淑环; 焦翔; 程永强

    2011-01-01

    研究了温度、pH值、空气、氧化剂、还原剂、碳水化合物及食品添加剂等不同条件对沙棘叶黄酮提取物水溶液稳定性的影响.结果表明,沙棘叶黄酮提取物水溶液在常温、中性及偏弱酸环境中具有相当高的稳定性,对一些常用的食品添加物质稳定,但应避免高温、强碱及氧化还原剂,应密封避光常温保存.%The stability of aqueous of sea buckthorn leaves flavonoids extracts under different conditions including temperature,pH, air, oxidizing agents, reducing agents, carbohydrates and food additives were studied. Results showed that aqueous of sea buckthorn leaves flavonoids extracts exhibited high stability at room temperature in neutral and weak acid environment. It was stable to some common food additives. But high temperature, alkali, oxidizing agents and reducing agents should be avoided and it should be stored at room temperature in a sealed dark.

  8. In vitro Evaluation of antibacterial efficacy of aqueous extracts of Iranian Native Plants on the Standard Strains of Pseudomonas aeruginosa (Short Communication

    Directory of Open Access Journals (Sweden)

    Saleh Jamehdor

    2014-08-01

    Results & Conclusion: In this study, the highest anti-bacterial effect have been relative to Hippophae rhamnoides (17.6 mm. The minimum inhibitory concentration of Zataria multiflora Boiss, Juglans regia L and Origanum vulgare was 25% and was recorded 12.5% for Hippophae rhamnoides. At this research, difined all of the herbal extracts have antibacterial effect on the studied strain and this activity due to the culture, origin, growing season and effective material in plants could be different.

  9. Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion

    Institute of Scientific and Technical Information of China (English)

    Santhrani Thakur; Pradeepthi Chilikuri; Bindu Pulugurtha; Lavanya Yaidikar

    2015-01-01

    Objective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were pretreated with alcoholic extract of H. salicifolia (250 and 500 mg/kg) for 14 d and focal cerebral ischemia was induced by MCAO. After 60 min of MCAO, reperfused for 24 h, a battery of behavioral tests were assessed the extent of neurological deficits. Infarct volume and brain edema were measured in 2,3,5-triphenyltetrazolium chloride stained brain sections. TNF-α, oxidative stress parameters like reduced glutathione, calcium, glutamate, malondialdehyde and apoptotic parameters like caspase-3, and caspase-9 were estimated in the brain homogenates. Results:Pretreatment with alcoholic extract of H. salicifolia at doses of 250 and 500 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema induced by ischemia reperfusion injury. H. salicifolia significantly prevented ischemia induced increase in malondialdehyde, glutamate, calcium, caspase-3, caspase-9 and TNF-αlevels as compared to ischemic animals. Conclusions: Our results indicate that H. salicifolia mitigated the ischemia reperfusion induced neuronal damage.

  10. Effect of Original Juice Treatment Before Fermentation on the Amino Acid of Sea-buckthorn and Raspberry Ice Wine%发酵前处理对沙棘树莓冰酒氨基酸的影响

    Institute of Scientific and Technical Information of China (English)

    马荣山; 郭宇

    2013-01-01

    Fruit wine was prepared by using sea-buckthorn and raspberry as raw materials, mixed fruit juice by D 101-I resin, processed orange juice and original juice were regulated to original sugar concentration of 12% and pH 4.2. Then added active dry yeast of 1.2‰,the process conditions of sea-buckthorn and raspberry wine fed-batch fermentation were optimized. In this study, the sea-buckthorn wine was detected after aging by the amino acid analyzer. It was found that The amino acid content of processed orange juice were relatively more.The sea buckthorn and raspberry wine browning can be controlled. Stability was improved, the taste was improved.%  以沙棘汁和树莓汁为原料,混合果汁经 D 101-I 大孔吸附树脂过滤,分别对处理过的果汁和未处理的果汁调整初始糖度12,pH4.2,接入1.2‰活性干酵母,采用流加发酵优化发酵工艺,经陈酿得到沙棘树莓冰酒。本实验采用 L-8800全自动氨基酸分析仪对两种发酵冰酒的氨基酸成分进行检测。结果表明发酵前对混合果汁用树脂处理,氨基酸含量相对多些,与此同时能有效控制沙棘树莓酒陈酿过程中的褐变,提高稳定性,改善酒的口感。

  11. Modulation of gamma ray induced chromosome aberrations in human peripheral blood lymphocytes by Hippophae rhammnoides leaf extract, SBL-1

    International Nuclear Information System (INIS)

    Hippophae rhammnoides L. commonly known as seabuckthorn is a temperate shrub and native of Asia and Europe. It has high antioxidant potential and is known to the traditional Indian, Chinese and Tibetan medicinal system for treatment of multiple disorders viz., circulatory and digestive disorders, hepatic injuries, neoplasia etc. One time treatment with the standardized leaf extract from H. rhammnoides (SBL-1) before whole body irradiation with 60Co (10 Gy), rendered more than 90% survival in non SBL-1 treated irradiated animals (J herbs, spices medi plants, 2009). Present study investigated the effects of SBL-1 treatment on chromosomal damage in human peripheral blood lymphocytes (PBL), with or without 60Co-gamma-radiation. The lymphocytes were isolated from the blood drawn from different donors. The isolated lymphocytes were divided into several groups: Group 1-untreated control, Group 2-irradiated (2 Gy), Group 3, 4 and 5 were treated with different concentration of SBL-1, 30 min. after irradiation with 60Co-gamma-rays (2 Gy). Group 6 was treated with the maximum concentration of SBL-1 used in the study. The metaphase spreading technique was used as per standard procedure to record chromosome breaks, dicentrics, acentrics and rings. The results were also recorded in terms of total aberrant metaphase and frequency of aberrant metaphase per 100 cells. In comparison to the untreated control, in the irradiated PBL culture, there was 8-fold increase in breaks, 211-folds in dicentrics, 75-folds in acentrics and 3-folds in rings (average data). SBL-1 alone at the highest concentration did not cause any significant change in number of breaks, dicentrics, acentrics and rings. The radiation induced aberrations decreased significantly by treatment with SBL-1 and the maximum decrease was observed when the cells were treated with 22μg/ml of SBL-1. These results demonstrated the anti-clastogenic activity of SBL-1 against gamma radiation induced damage. (author)

  12. 微波消解-双道原子荧光光度计测定沙棘汁、沙棘果粉中的铅、砷、汞%Microwave Digestion-Double Line AFS Determination of Trace Lead, Arsenic and Mercury in Sea-buckthorn Juice and Sea-buckthorn-fruit-powder

    Institute of Scientific and Technical Information of China (English)

    汪正花; 段金凤; 师存杰

    2013-01-01

    Trace lead, arsenic and mercury in sea-buckthorn juice and sea-buckthorn-fruit-powder were determined by using microwave digestion-double lines AFS. The working conditions of instrument were discussed as well as influential factors including the sample digestion condition, potassium borohydride dosage, concentration and kinds of acids. The results show that detection limit of the method for each element is 0.000 1, 0.000 2, 0.000 02 mg/kg, respectively;The RSD is in the range of 1.20%~4.83%and the recoveries are in the range of 92%~105%.%采用微波消解技术-双道原子荧光法测定了沙棘汁、沙棘果粉中的痕量元素铅、砷、汞。对仪器的工作条件作了详述,还就各种对测定有影响的因素,包括样品消解条件,硼氢化钾的浓度、介质的种类及浓度等也作了研究并予以优化选择。方法对这3种元素的检出限为0.0001,0.0002,0.00002 mg/kg,相对标准偏差为1.2%~4.83%,用加标回收率评价方法的准确性,回收率为92%~105%。

  13. Comparative Fingerprint of Aromatic Herbs and Yeast Alcoholic Extracts used as Ingredients for Promen, a Prostate Preventive Nutraceutical

    Directory of Open Access Journals (Sweden)

    Florina Csernatoni

    2013-11-01

    Full Text Available The aim of this study was to characterize and identify different bioactive compounds in plant sources and yeast powders  to obtain an original nutraceutical (Promen which has beneficial effects in prostate disease prevention. Seven plant and fruit sources, namely nettle (Urtica dioica, green tea (Camellia sinensis, fluff with small flowers (Epilobium parviplorum, tomato (Solanum licopersicum,  sea buckthorn (Hippophae rhamnoides, pumpkin (Cucurbita maxima, sunflower (Helianthus annus and lyophilized beer yeast (Saccharomyces cerevisiae were investigated. Methanolic extracts were prepared using 15% plant concentration and the purified fractions were analyzed using high throughput techniques like UV-VIS spectroscopy, high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD and mass spectrometry LC-QTOF -MS. The majority of the investigated plants were rich in phenolic derivatives, polyphenols (flavonoid glucosides, while yeast was rich in aminoacids, peptides and vitamins B. The major compounds identified were: Juglone, Resveratrol, Quercetin, Epigallocatechin, Gallocatechin, Biochanin A, Isorhamnetin 3-O-glucoside 7-O-rhamnoside, Quercetin 3-O-galactoside 7-O-rhamnoside, Kaempferol 3,7-O-diglucoside and p-Coumaroylquinic acid. The specific biomarkers were identified for both plant extracts used as ingredients to obtain an nutraceutical  Promen. Combined UV-Vis spectroscopy, HPLC-PDA chromatography and LC-MS spectrometry are recommended as accurate, sensible and reliable tools to investigate the plants and nutraceutical fingerprints and to predict the relation between ingredients composition and their health effects.

  14. Cooperative antiproliferative and differentiation-enhancing activity of medicinal plant extracts in acute myeloid leukemia cells.

    Science.gov (United States)

    Zhamanbayeva, Gulzhan T; Aralbayeva, Araylim N; Murzakhmetova, Maira K; Tuleukhanov, Sultan T; Danilenko, Michael

    2016-08-01

    Acute myeloid leukemia (AML) is an aggressive hematopoietic malignancy with poor prognosis and limited treatment options. Sea buckthorn (Hippophae rhamnoides) berries, dog rose (Rosa canina) rosehips, and garden sage (Salvia officinalis) and oregano (Origanum vulgare) aerial parts are widely used in traditional medicine and exhibit antitumor effects in preclinical models. However, these plants remain scarcely tested for antileukemic activity. Here, we show that their water-ethanol leaf extracts reduced the growth and viability of AML cells and, at non-cytotoxic doses, potentiated cell differentiation induced by a low concentration of 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D, in a cell type-dependent manner. The latter effect was accompanied by upregulation of the vitamin D receptor protein components and its transcriptional activity. Furthermore, at minimally effective doses the extracts cooperated with one another to produce marked cytostatic effects associated with a partial S-phase arrest and a modest induction of apoptosis. In contrast, these combinations only slightly affected the growth and viability of proliferating normal human peripheral blood mononuclear cells. In addition, the extracts strongly inhibited microsomal lipid peroxidation and protected normal erythrocytes against hypoosmotic shock. Our results suggest that further exploration of the enhanced antileukemic effects of the combinations tested here may lead to the development of alternative therapeutic and preventive approaches against AML. PMID:27470342

  15. The Influence of Some Phytobiotics on Haematological and Some Biochemical Indices at Oreochromis Niloticus – Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Alina Antache

    2014-05-01

    Full Text Available The aim of this research was to evaluated the influence of some phytobiotics on haematological profile, leukocyte reaction and some biochemical indices at Oreochromis niloticus species, reared in a recirculating aquaculture system. This experiment was conducted six weeks. The experimental variants were: V1 – control; V2 – 1% Rosmarinus officinalis / kg feed; V3 – 1% Hippophae rhamnoides / kg feed and V4 – 1% Zingiber officinale / kg feed. Blood was analyzed using standard techniques. At the end of the experiment the following parameters were determined: RBCc (x106cells/µL, Hb (g/dL, PVC (%, MCV (µm3, MCH (pg, MCHC (g/dL, TP (g/dL, GLU (mg/dL, cortisol (ng/mL, lysozyme activity (U/mL, absolute number of blood cells (x103 cells/µL and leukogram (%. The results showed that the administration in feed of some phytobiotics lead to signifiant differences (p<0.05 of following parameters: RBCc (x106cells/µL, MCV (µm3, glucose (mg/dL, lysozyme activity (U/mL, monocyte (% and in absolute number of leukocytes, lymphocytes and monocytes. In conclusion, due to decreasing of RBCc, PVC, Hb, MCHC, cortisol, GLU and due to normal concentration of TP, we can say that the administration of sea buckthorn and ginger, but even rosemary administration, in diet improves the physiological status at Oreochromis niloticus species.

  16. Enhanced adsorptive removal of Safranine T from aqueous solutions by waste sea buckthorn branch powder modified with dopamine: Kinetics, equilibrium, and thermodynamics

    Science.gov (United States)

    Xu, Xiaohui; Bai, Bo; Wang, Honglun; Suo, Yourui

    2015-12-01

    Polydopamine coated sea buckthorn branch powder (PDA@SBP) was facilely synthesized via a one-pot bio-inspired dip-coating approach. The as-synthesized PDA@SBP was characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The adsorption progresses of Safranine T on the surface of PDA@SBP adsorbent were systematically investigated. More specifically, the effects of solution pH, contact time, initial concentration and temperature were evaluated, respectively. The experimental results showed the adsorption capacity of PDA@SBP at 293.15 K could reach up to 54.0 mg/g; the adsorption increased by 201.7% compared to that of native SBP (17.9 mg/g). Besides, kinetics studies showed that pseudo-second-order kinetic model adequately described the adsorption behavior. The adsorption experimental data could be fitted well a Freundlich isotherm model. Thermodynamic analyses showed that the ST adsorption was a physisorption endothermic process. Regeneration of the spent PDA@SBP adsorbent was conducted with 0.1 M HCl without significant reduction in adsorption capacity. On the basis of these investigations, it is believed that the PDA@SBP adsorbent could have potential applications in sewage disposal areas because of their considerable adsorption capacities, brilliant regeneration capability, and cost-effective and eco-friendly preparation and use.

  17. Physicochemical properties changes of sea buckthorn cloudy juice during cold crushing, concentrating and storage%加工过程中冷打浆沙棘浓缩浊汁理化特性的变化

    Institute of Scientific and Technical Information of China (English)

    宋自娟; 徐怀德; 高锦明; 张明兰; 肖蕊; 李文娟

    2014-01-01

    Sea buckthorn berries were used as raw materials to study the process of concentrating sea buckthorn cloudy juice and their quality. Physicochemical properties including vitamin C, total flavonoids, total polyphenols, polysaccharides, pH value, total acidity, and browning index changed. Cold crushing sea buckthorn berries, concentrating production and the storage of concentrated cloudy juices were investigated systematically. The results showed that the quality of sea buckthorn juice obtained by cold crushing process technology was excellent. The contents of soluble solids and total acidity reached to 18.0% and 4.24% of sea buckthorn juice obtained respectively. It contained vitamin C 729.69 mg/100mL, flavonoids 445.17 mg/100mL, total polyphenols 924.67 mg/100mL and polysaccharides 25.22 mg/100mL. Disc milk separator removed the impurities and oil in sea buckthorn juice due to horizontal screw centrifuge. Whole cold crushing was fast with low temperature. Nutrient and health components in sea buckthorn juice lost less, they had been effectively enriched. Then, different concentrations for multiple sea buckthorn juices were processed at 45℃ by evaporation in the vacuum of 0.095~0.1 MPa. With the increase of the concentration, the loss rate of nutrients and browning index became larger, the total acidity and 5-HMF content were increased and pH value was decreased. Different concentratedn juices were placed at room temperature (10 to 25℃) and refrigerated conditions (5 to 8℃) for 60 days, their physicochemical properties were changed as follows. Vitamin C, flavonoids, total polyphenols and polysaccharide contents decreased gradually, pH and total acidity did not change significantly, browning index and 5-HMF content increased. Furthermore, refrigerated conditions were conducive to the preservation of the sea buckthorn juice compared with the room temperature condition. The nutrient and health components in preserving rate of same multiple sea buckthorn cloudy

  18. Study on the anti-tumor experiment by using Sea buckthorn oil intravenous emulsion associated with cyclophosphamide%沙棘油静脉乳联合环磷酰胺抗肿瘤实验研究

    Institute of Scientific and Technical Information of China (English)

    陈世虎; 惠爱武; 罗娟; 李义; 杨甫昭; 范引科; 刘杏

    2011-01-01

    OBJECTIVE To study the effect of Sea buckthorn oil intravenous emulsion on enhancing the efficacy of chemotherapy and reducing its side-effect in mice inoculated with S180 tumorous cells. METHODS The mice inoculated with S180 tumorous cells were divided into four groups: S180 control group, CTX group, Sea buckthorn oil intravenous emulsion group (20,10,5 rnL· kg-1 ) , Sea buckthorn oil intravenous emulsion (20,10,5 mL·kg- 1 ) associated with CTX groups. After 10 days treatment, calculated the tumor inhibitory rate, and evaluated the counting of peripheral blood cells and nucleus cells in marrow, the weight of spleen and thymus, reticuloendothelial system, blood flow in the inert carbon particles on phagocytosis clearance capacity. RESULTS The tumor inhibitory rate was higher in each Sea buckthorn oil intravenous emulsion associated with CTX group than that in the S180 control group (P<0. 01 or P<0. 05). The tumor inhibitory rate of each Sea buckthorn oil intravenous emulsion associated with CTX group was higher than CTX group(P<0. 05) . The numbers of nucleus cells in marrow, WBC and the indices of spleen and thymus in CTX group were lower than those of S180 control group. When compared with CTX group, moderate and large dose Sea buckthorn oil intravenous emulsion associated with CTX could obviously increase the numbers of nucleus cells in marrow, the numbers of peripheral blood leukocytes and spleen index (P<0. 01 or P<0. 05). The phagocytosis coefficient of Sea buckthorn oil intravenous emulsion (20 mL· kg-1 ) was obviously higher than the control group (P<0. 05). It could enhance the clearance capacity of carbon particles in mice significantly. CONCLUSION Sea buckthorn oil intravenous emulsion can enhance the efficacy of chemotherapy and reduce its side-effect in mice inoculated with S180 tumor cells exposed to CTX. Sea buckthorn oil intravenous emulsion can improve the non-specific immune function significantly.%目的:探讨沙棘油静脉乳

  19. 二倍体杂交种棱果沙棘双向杂交起源及其母本主要来源于中国沙棘的分子证据%Molecular Evidence for Bidirectional Hybrid Origin and Hippophae rhamnoides ssp.sinensis as the Mainly Maternal Plant of the Diploid Hybrid H.goniocarpa (Elaeagnaceae)

    Institute of Scientific and Technical Information of China (English)

    蒋严妃; 严容; 苏雪; 陈纹; 孙坤

    2014-01-01

    棱果沙棘为同域分布的中国沙棘和肋果沙棘同倍化自然杂交形成的.本文利用母系遗传的cpDNA trnS-G序列检测青海祁连棱果沙棘及其亲本中国沙棘和肋果沙棘同域分布的两个地区(拱北湾、八宝河滩)共93个个体的遗传关系.结果表明棱果沙棘及其亲本在拱北湾和八宝河滩分别有12个和7个单倍型,两地区的棱果沙棘都与其亲本共享单倍型,其中拱北湾棱果沙棘共36个个体中有28个与中国沙棘共享3个单倍型(H2,H4,H5),有2个个体与肋果沙棘共享单倍型(H11),八宝河滩的棱果沙棘共10个个体中有7个与中国沙棘共享一个单倍型(H4),3个与肋果沙棘共享单倍型(H7).应用最大简约法(MP)分别对两地区的棱果沙棘及其亲本trnS-G序列构建的系统发育树中棱果沙棘的大部分个体都与中国沙棘聚在一起,另外,棱果沙棘4种特有单倍型(H3、H7、H8、H9)的6个个体在系统树上也与中国沙棘聚为一支.以上结果进一步证明了二倍体自然杂交种棱果沙棘为双向杂交起源,但其主要母本来源应为中国沙棘.

  20. Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation%栽培模式对沙棘人工林土壤微生物群落结构和参与氮循环功能基因的影响

    Institute of Scientific and Technical Information of China (English)

    杨丹; 余旋; 刘旭; 刘金良; 张顺祥; 于泽群

    2015-01-01

    以沙棘×油松混交林、沙棘×侧柏混交林、沙棘×刺槐混交林、沙棘纯林的土壤为研究对象,对其土壤微生物种群结构、参与氮循环功能基因丰度进行了检测.结果表明:沙棘与油松或侧柏混交能显著增加林地土壤总磷脂脂肪酸(总PLFA)、细菌脂肪酸(BPLFA)和革兰氏阳性菌脂肪酸(G+PLFA)含量,而真菌脂肪酸(FPLFA)含量无明显变化.两种混交林地土壤的nifH、amoA、nirK和narG基因丰度明显高于沙棘纯林土壤.土壤总PLFA、G+PLFA、革兰氏阴性菌生物量(G-PLFA)和4种功能基因的丰度均与土壤pH、土壤有机碳、总氮、NH4+-N、速效钾呈显著正相关.不同栽培模式人工林土壤微生物群落和基因丰度的差异主要与土壤理化特性有关.沙棘与油松或侧柏混交为当地2种较好的栽培模式,能有效地改善土壤质量.

  1. Research on Quality Standards of Fruit of Hippophae Tibetana Schlechtend%西藏沙棘质量标准研究

    Institute of Scientific and Technical Information of China (English)

    杨洋; 苏永文; 刘悦; 游佳莉; 张艺; 王毓杰

    2014-01-01

    目的:建立西藏沙棘药材质量标准。方法:采用显微法及TLC法鉴别西藏沙棘药材;按照2010年版《中国药典》(一部)测定西藏沙棘药材水分、总灰分、酸不溶性灰分及醇溶性浸出物含量;采用HPLC法测定其槲皮素、山柰素及异鼠李素含量。结果:确定西藏沙棘药材显微特征,建立槲皮素、山柰素及异鼠李素3种黄酮类成分的薄层鉴别及含量测定方法。结论:本研究建立的定性、定量方法,可用于西藏沙棘药材质量控制。%This study was aimed to establish quality standards of fruit of Hippophae tibetana Schlechtend.. The medical material was identified by microscopy and thin layer chromatography (TLC). According to methods recorded in the Chinese Pharmacopoeia (2010 edition), the contents of moisture, total ash, acid-insoluble ash and alcohol-sol-uble extract were determined. The HPLC method was applied in the determination of quercetin, kaempferol and isorhamnetin content. The results showed that the microscopic characteristics of Tibetan medicine H. tibetana were identified. The TLC and content determination methods of quercetin, kaempferol and isorhamnetin were estab-lished. It was concluded that the established qualitative and quantitative methods can be used for quality control of the fruit of H . tib e tana.

  2. 沙棘微扦插不定根发生的形态解剖学研究%Morphological and anatomical features during the formation of adventitious roots of sea buckthorn by micro-cuttage multiplication

    Institute of Scientific and Technical Information of China (English)

    姚景瀚; 李伟

    2013-01-01

    利用石蜡切片法对沙棘微扦插不定根的发生进行形态解剖学研究.结果表明:沙棘属于诱导型生根,观察到其不定根原基起源于2个区域,一部分根原基起源于维管形成层细胞,另一部分起源于韧皮部薄壁细胞区域.沙棘不定根原基继续发育时,一部分沿射线向外生长,另一部分向外生长的同时会与茎段的髓连接起来,这种现象在其他树种中尚未发现.沙棘插穗不定根的发生整体上可分为4个阶段:1)维管形成层细胞和韧皮部薄壁细胞分裂出一些排列紧密、细胞质浓、具有分生组织特点的薄壁细胞团;2)新形成的薄壁细胞团不断分裂并且脱分化,形成具有很强分生能力的不定根原基发端细胞;3)根原基发端细胞继续分裂,沿着射线向远离轴心的方向伸展,从而形成根原基;4)根原基穿过韧皮射线和皮层,向外继续朝皮孔方向发展,最终从皮孔伸出形成不定根,不定根伸出皮孔前内部维管系统发育完善并与茎部维管系统相连接.%Morphological and anatomical features were observed during the formation of adventitious roots of sea buckthorn by micro-cuttage multiplication with paraffin method. The results showed that adventitious root primordium originated from two areas ( one was cell of vascular cambium, and the other was cell from cortex parenchyma) was inductive root formation type. When the adventitious root of sea buckthorn continued to grow, this phenomenon that one of its part extended out in ray direction and the other part would connect with the marrow of stem while it was stretching out remained undiscovered in other species. In general, formation of adventitious root of sea buckthorn can be divided into four stages: 1 ) The cells of cortex parenchyma and vascular cambium divided into parenchyma cell groups which contained the characteristics of meristem, stood in a line and bore dense cytoplasm concentration. 2 ) Newly formed parenchyma

  3. 将沙棘食品应用军队饮食保障探索%Will Sea Buckthorn Food Applications in the Food Supply of the Armed Forces to Explore

    Institute of Scientific and Technical Information of China (English)

    刁子蔚; 刘崇彦

    2013-01-01

    沙棘果享有第三代水果“VC之王”美誉。沙棘食品应用军队饮食保障,既可以丰富官兵果蔬品种,食用又可以辅助解决军人由于职业的特性维生素流失大、各种职业病肠胃、慢性便秘、口腔溃疡、高原缺氧引发咳嗽哮喘等突显的难题。文章围绕军用食品条件要求,从军人食物定量新标准、全国19个省市种植面积果实年收获量,沙棘食品深加工方法、质量保证手段、国内沙棘食品系列现状、沙棘食品性价比、适用官兵群体等方面论证了沙棘食品应用军队饮食保障体系符合性。建议沙棘原果、饮料、罐头、速溶饮料和烹饪食材应用军队饮食。%Seabuckthorn fruit for third- generation fruit 'the VC king' of reputation. Seabuckthorn food applications in the food supply in the military , both officers and men of fruit and vegetable varieties can enrich food they can assist to solve the soldiers vitamin loss due to occupational characteristics , a variety of occupational gastrointestinal, chronic constipation, mouth ulcers, high altitude hypoxia caused by cough, asthma and other highlights problem. The article focuses on the conditions of military food requirements , new standards of the military food ration , the country's 19 provinces and cities acreage fruit harvest , sea buckthorn food processing methods, quality assurance measures, the status of domestic sea buckthorn food series, seabuckthorn food cost-effective, applicable to officers and men groups such as the compliance of the seabuckthorn food application of the military diet security system. The original proposal seabuckthorn fruit , drinks, canned food, instant drinks and cooking ingredients application of the military diet.

  4. Differences in hydrological responses for different vegetation types on a steep slope on the Loess Plateau, China

    Science.gov (United States)

    Duan, Liangxia; Huang, Mingbin; Zhang, Luodan

    2016-06-01

    Extensive vegetation restoration practices have been implemented to control soil erosion on the Loess Plateau, China. However, no strict guidelines are available to determine the most suitable plant species for vegetation restoration within a given area. The objective of this study was to quantify the changes of each component (soil water storage, surface runoff, and actual evapotranspiration) of a water balance model and soil loss over time under eight different vegetation types, and to further determine the optimal vegetation type for soil and water conservation and sustainable ecological restoration on the steep slopes (>25°) on the Loess Plateau. The results indicated that vegetation type substantially affected soil water storage and that the greatest soil water storage in both the shallow (0-2 m) and the deep soil layers (2-5 m) occurred under Bothriochloa ischaemum L. (BOI). Vegetation type also affected surface runoff and soil losses. The most effective vegetation types for reducing soil erosion were BOI and Sea-buckthorn (Hippophae rhamnoides L.), while Chinese pine (Pinus tabulaeformis Carr.) and Chinese pine + Black locust (Robinia pseudoacacia L.) were the most ineffective types. Soil water dynamics and evapotranspiration varied considerably among the different vegetation types. A soil water surplus was only found under BOI, while insufficient water replenishment existed under the other seven vegetation types. The higher water consumption rates of the seven vegetation types could result in soil desiccation, which could lead to severe water stresses that would adversely affect plant growth. This study suggested that both vegetation type and its effect on controlling soil erosion should be considered when implementing vegetation restoration and that BOI should be highly recommended for vegetation restoration on the steep slopes of the Loess Plateau. A similar approach to the one used in this study could be applied to other regions of the world confronted

  5. Antimicrobial Effect of Escherichia Coli on Essential Oils Derived from Romanian Aromatic Plants

    OpenAIRE

    Şandru Daniela Maria

    2015-01-01

    This paper investigates the antimicrobial action of Escherichia coli ATCCR CRM-8739TM on the following essential oils: Teucrium marum, Pinus sylwestris, Thymus vulgaris, Salviae aethedaroleum, Cinnamomum aromaticum, Hippophae rhamnoides, Lavandula angustifolia, Abies alba, Zingiber officinale, Anethum graveolens, Coriandrum sativum, Origanum vulgare, extracted industrialy from romanian plants, using the diffusion disc method. The most intense activity was observed at the essential oil of Cinn...

  6. Optimization of Extraction Program of Sea Buckthorn Seeds Proanthocyanidins by Combining Uniform Design and Microsoft Excel%联合均匀设计法和Microsoft Excel优化沙棘籽原花色素提取工艺(英文)

    Institute of Scientific and Technical Information of China (English)

    田亚新; 韩晓云; 王运来; 康传红

    2012-01-01

    A novel optimization method based on uniform design and function LINEST of Microsoft Excel was proposed to explore the extraction of proanthocyanidins from sea buckthorn seeds. The proposed multiple regression equation was calcu- lated with the function LINEST. This method was not only a flexible method, but also could avoid high expenditure and fixed algorithm while using other professional software for data processing. The validation experiments showed that the extraction rate of proanthocyanidins from sea buckthorn seeds revealed an increase by 16.43%. This method is easy to extend and may hold promise for other scientific applications.%提出一个基于均匀设计方法和Microsoft Excel的LINEST函数进行试验设计优化的方法。本实验通过均匀设计方法设计沙棘籽原花色素提取工艺,然后通过LINEST函数计算多元回归方程,该方法可避免因为使用其他专业数据处理软件的花费和固定算法限制。验证实验表明:沙棘籽原花色素的提取率提高了16.43%,该方法可应用到其他实验方面。

  7. 黄土丘陵区不同造林模式的土壤效应研究%Soil Effects of Different Afforestation Model in Hilly Loess Plateau

    Institute of Scientific and Technical Information of China (English)

    李金花

    2012-01-01

    分析青海黄土丘陵区相同林龄(8年)的5种造林模式土壤的有机质含量、总孔隙度和微生物总量大小,结果为云杉桦树混交〉桦树〉云杉沙棘混交〉云杉〉沙棘〉农田;各造林模式缓解日温变化效应的大小顺序为云杉桦树混交〉桦树〉云杉沙棘混交〉沙棘〉云杉〉农田。%The effects of soil from different afforestation models in hilly loess plateau were analyzed. The results were as follows: mixture of picea crassifolia and birch 〉birch 〉mixture of picea crassifolia and hippophae rhamnoides 〉picea crassifolia 〉hippophae rhamnoides〉farmland. The order of easing temperature changes was as follows: mixture of picea crassifo|ia and birch 〉birch 〉mixture of picea crassifoIia and hippophae rhamnoides ~hippophae rhamnoides 〉picea crassifo|ia 〉 farmland.

  8. Pěstování, sklizeň a zpracování rakytníku řešetlákového (Hippophäe rhamnoides) na půdách v LFA a návrh užitného vzoru výroby bylinného léčivého likéru z jeho plodů ve směsi s plody bezu černého (Sambucus nigra).

    OpenAIRE

    HAVLÍKOVÁ, Marcela

    2016-01-01

    The hereby work describes a new product - herbal liquor containing antioxidants of elderberry and sea buckthorn. It is completely designed, manufactured and tested by a sensory analysis. I performed everything using a simple, low capital technologies, and raw materials, which could easily grow on soils in LF areas. Thus would these LF areas have an appropriate industrial applications . Since LF areas are often areas with high unemplyements I think the realization of such production could brin...

  9. Diversity, distribution and vegetation assessment in the Jahlmanal watershed in cold desert of the Lahaul valley, north-western Himalaya, India

    OpenAIRE

    Rawat YS; Vishvakarma SCR; Oinam SS; Kuniyal JC

    2010-01-01

    Relict vegetation in the Jahlmanal watershed is sparsely and scantily distributed on forest land. Farmers maintain tree species (Salix spp., Populus spp., Hippophae rhamnoides) on the boundaries of terraced agriculture fields to meet the fodder and fuel wood requirements. Juniperus macropoda was found in relict forest patches in sparse and stunted conditions. The density of Salix fragilis was 3340 trees ha-1 under agroforestry and 5520 trees ha-1 under forestry. The density of J. macropoda wa...

  10. Clinical and sebumetric evaluation of topical emulsions in the treatment of acne vulgaris

    OpenAIRE

    Khan, Barkat Ali; Akhtar, Naveed

    2014-01-01

    Introduction Numerous plant products described in the scientific literature show distinct activities on the skin, such as moisturizing, antioxidant, sunscreen, anti-acne and depigmentation. Aim The main objective of this study was to compare the effectiveness of emulsion formulations containing plant extracts (Hippophae rhamnoides and Cassia fistula) and placebo (without plant extracts) on acne patients. Material and methods A single-blind, randomized, placebo-controlled, split-face study was...

  11. Developing technology of remediation of oil-contaminated soils

    OpenAIRE

    Shevchyk, Lesya; Romaniuk, Olga

    2013-01-01

    Abstract ? The results of developing technologies for cleaning of soils from oil pollution on the example of Boryslav are shown. The prospects of tree species for the remediation of oil-contaminated soils are studied. The best results of cleaning oil contaminated soils with the application of Hippophae rhamnoides L. plants were obtained. It is a promising measure for restoring the oil-contaminated soils, attractive both from environmental and economical point of view.

  12. Nutritional profile of phytococktail from trans-Himalayan plants.

    Directory of Open Access Journals (Sweden)

    Priyanka Dhar

    Full Text Available We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides, apricot (Prunus armeniaca, and roseroot (Rhodiola imbricata from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS. Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID with fatty acid methyl esters (FAMEs derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs, palmitoleic acid (43.47%, oleic acid (20.89%, and linoleic acid (4.31% were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES, and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit

  13. Genetic diversity and genetic structure of the northern margin populations of Hippophae neurocarpa%肋果沙棘北缘居群的遗传多样性与遗传结构

    Institute of Scientific and Technical Information of China (English)

    李霓; 蒋严妃; 苏雪; 陈纹; 张辉; 孙坤

    2016-01-01

    We analyzed five natural populations of Hippophae neurocarpa in the Qilian area of Qinghai Province with SSR molecular markers, in order to understand the genetic diversity of the northern margin populations of H. neurocar-pa on the small geographic scales and the genetic structure of the populations which distribute fragmentally, and to pro-vide reference for resource protection of H. neurocarpa populations. We used six microsatellite primers to amplify the DNA of 107 samples and detected 27 alleles, with the range of 2-9 and a mean value of 4.67 per locus. The average observed heterozygosity ( Ho) and expected heterozygosity ( He) of H. neurocarpa populations was 0.142 and 0.230 re-spectively, and the range Shannon information index (I) was between 0.280-0.567 with a average value of 0.374. The results showed that the genetic diversity of the northern margin populations of H. neurocarpa was abundant. The coeffi-cient of genetic differentiation ( Fst) was 0.483. Molecular variance analysis ( AMOVA) indicated that 48.33% of the variation existed among the populations, and 51.67%existed within the populations. No significant correlation between genetic distance and geographic distance was detected by Mental test and the detection of gene flow found that Nm was only 0.328 among populations. These results suggested that genetic drift was one of the key factors for the differentia-tion of populations. The 5 populations were divided into 2 groups based on the structure analysis. UPGMA clustering showed that the 5 populations were clustered into 2 branches, one of which was ARX, which was consistent with the results of the principal coordinate analysis. According to the analysis of the genetic structure, it is suggested to protect populations in situ as many as possible.%该研究利用SSR分子标记,对肋果沙棘( Hippophae neurocarpa)分布区北缘青海祁连地区5个自然居群进行分析,旨在了解小地理尺度下肋果沙棘北缘居群的遗传多样

  14. 中国沙棘克隆生长对不同光照强度的响应%The Clonal Growth of Hippophae rhamniodes L.subsp.sinensis in Response to Different Light Intensity

    Institute of Scientific and Technical Information of China (English)

    唐翠平; 郭峰; 徐德兵; 贺斌; 李甜江; 李根前

    2014-01-01

    The clonal growth of Hippophae rhamniodes L.subsp.sinensis in response to different light intensity (100%, 70 %, 50%and 20%of nature light ) by using shading net was studied , and the results showed: 1 ) The pop-ulation growth, the biomass accumulation and its distribution , underground clonal growth parameters , and ect. were all showed a change of quadratic parabolic curve with the light intenstity increasing from 20%to 100%, and the best light intenstity for clonal growth was 50%~70%.2 ) The optimum light intensity for population growth , biomass of community and different organs , daughter ramet number and spacer growth were 48.0 %~61.7 %, 52.8 %~53.6 %, and 55.6%~57.6% respectively , and there was a cooperative function and a trade-off re-lation among them .3 ) At the optimum light intenstity , the population was characterised by individual tall and concentrated distribution , and it growth partern tended to a phalanx type .For too much or too small light intensi-ty, the population was characterized by individual low and dispersed distribution , and its growth pattern tended to a guerrilla type .The population growth patterns would change from guerrilla type to phalanx type , and then to guerrilla type , and formed a foraging strategies continuum with the increasing of light intensity .%利用遮阳网产生的不同光照强度(100%、70%、50%和20%的自然光照),研究了中国沙棘克隆生长对不同光照强度的响应规律。结果表明,(1)当光照强度从20%逐渐增加到100%时,种群生长量、生物量积累与分配、地下克隆器官参数等增幅均呈二次抛物线变化,相对光照强度为50%~70%是其克隆生长的最佳光照强度。(2)种群生长量、种群及各器官生物量、子株数量及萌蘖根生长量增幅最大时的光强分别为48.0%~61.7%、52.8%~53.6%、55.6%~57.6%,且分株生长量和种群生物量、萌蘖根生长量和子株数量之间

  15. 三波长HPLC同时测定沙棘颗粒中的槲皮素、山萘酚与异鼠李素%Simultaneous quantitative determination of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids granules under tri-wave length by RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    甘盛; 韩婷; 施晓光; 吴超权

    2013-01-01

    目的 用HPLC法在3个波长段同时测定沙棘颗粒中槲皮素、山萘酚与异鼠李素的含量.方法 经室温两步提取处理沙棘颗粒中的黄酮类化合物,测定其中的槲皮素、山萘酚与异鼠李素.在反相液相系统中,采用Symmetry C18柱(250 mm×4.6mm,5.0 μm),流动相为乙腈-0.4%磷酸溶液(40∶60),室温,流速1.0 mL· min-1.槲皮素、山萘酚、异鼠李素的检测波长分别为266、360、370 nm.结果 槲皮素、山萘酚与异鼠李素均在0.2~1.6 μg与峰面积线性关系良好,平均回收率分别为99.7%、98.3%、101.0%,r分别为0.9993、0.9996、0.9991.结论 所用方法灵敏度高、专属性强,适用于测定复方沙棘颗粒中的槲皮素、山萘酚与异鼠李素的含量.%OBJECTIVE To assay the content of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids Linn. granules under tri - wave length by HPLC. METHODS Flavone glycosides were extracted in two steps from hippohae rhamnoids Linn. granules , in which the quercetin, kaempferol and isorhamnetin content were assayed. The RP - HPLC workstation equipped with the Symmetry C18 column (250 mm ×4. 6 mm, 5. 0 μm) at room temperature was used, the mobile phase was composed of acetotrile and 0. 4% of phosphoric acid (4 : 6) with a flow rate of 1. 0 mL·min-1. Quercetin was detected at 266 nm, while kaempferol at 360 nm and isorhamnetin at 370 nm. RESULTS The good linearity was obtained at the concentration of 0. 2 - 1. 6 μg, the average recoveries were 99. 7% ,98. 3% and 101.0% , with the relative coefficients of 0. 9991,0. 9994 and 0. 9990, respectively. CONCLUSION The method is proved to be sensitive and specific, applicable for the determination of quercetin, kaempferol and isorhamnetin in Hippohae rhamnoids Linn, granules.

  16. Effect on Soil and Water Conservation from Different Models of Conversion of Farmland to Forest in Huangshui River at Qinghai%青海湟水河流域不同退耕还林模式水土保持效应

    Institute of Scientific and Technical Information of China (English)

    赵串串; 董旭; 辛文荣; 王湜; 杨乔媚

    2009-01-01

    Based on field investigation and experimental data, soil and water conservation effect was studied in Huangshui river at Qinghai province. The results showed that the sequence of water and soil holding capacity is 41 a Populus cathayana>41 a Picea crassifolia>natural scrub forest>9 a mixture of Picea crassi folia and Hippophae rhamnoides >9a Hippophae rhamnoides >9 a Picea crassi folia (lower slope) >9 a Picea crassi folia (upper slope) > 4 a mixture of Picea crassi folia and Hippophae rhammnoides > farmland. Cam-pared with farmland soil, after 5 years of conversion of farmland to forest, the index of physical and chemical characters and microbial characteristic in soil, takes on obvious changes, and it is stable infiltration velocity of natural scrub forest>9 a mixture of Picea crassi folia and Hippophae rhamnoides>4 a mixture of Picea crassi folia and Hippophae rhamnoides > farmland. And soil erodible factor, K is determined to 0. 483 in natural scrub forest from 0. 773 in farm. And water holding of little per unit of 41 a Populus cathayana was 2. 6 times than that of 41a Picea crassi folia.%基于野外定点观测资料收集和室内实验分析,研究了青海湟水河流域不同退耕还林模式下水土保持效应,结果表明:41 a青杨>41 a云杉>天然次生灌木林>9 a云杉沙棘混交>9 a沙棘>9 a云杉(坡位下)>9 a云杉(坡位上)>4 a云杉沙棘混交>农田.同时对照农耕地土壤:退耕还林地土壤理化性质和生物特性在退耕5 a后有明显变化,天然次生灌木林稳渗率>9 a云杉沙棘混交>4 a云杉沙棘混交>农田;土壤可蚀性因子从农耕地的0.773减少到次生灌木林的0.483;41 a青杨枯落物单位面积有效蓄水量是41 a云杉的2.6倍.

  17. In vitro antioxidant profiling of seabuckthorn varieties and their adaptogenic response to high altitude-induced stress

    Science.gov (United States)

    Sharma, Priyanka; Suryakumar, Geetha; Singh, Virendra; Misra, Kshipra; Singh, Shashi Bala

    2015-08-01

    In the past few years, seabuckthorn plants have gained special attention due to their ability to grow in the harshest of the environment. This adaptability may be contributed by various antioxidants present in the plants besides other morphological adaptation. As in vivo studies cannot be justified without in vitro studies, the present investigation carried out evaluation of both in vitro and in vivo antioxidant potentials of aqueous and alcoholic extracts of the leaves of Hippophae salicifolia (HS) and Hippophae rhamnoides mongolica (HRM) in comparison with Hippophae rhamnoides turkestanica (HRT). The results had clearly depicted that in vitro antioxidant potential of the extracts was responsible for the in vivo adaptogenic performance in animals during cold and hypoxia exposure under restraint stress. Total phenolic content (TPC), total flavonoid content (TFC), total protein content, and antioxidant potential were determined. For adaptogenic studies, rats with oral drug supplementation were exposed to Cold-hypoxia-restraint (C-H-R) stresses-induced hypothermia, as a measure of endurance. Aqueous extracts of HS showed maximum (99 %) resistance compared to HRT (81 %) and HRM (29 %). The levels of biochemical parameters such as malondialdehyde (MDA), reactive oxygen species (ROS), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione (GSH/GSSG), and catalase (CAT) in blood samples also revealed that the aqueous leaf extract of HS has better antioxidant and adaptogenic potential compared to HRM.

  18. [Root anatomical structure and hydraulic traits of three typical shrubs on the sandy lands of northern Shaanxi Province, China].

    Science.gov (United States)

    Ai, Shao-shui; Li, Yang-yang; Chen, Jia-cun; Chen, Wei-yue

    2015-11-01

    Root xylem anatomical structure and hydraulic traits of three typical shrubs, i.e., Salix psammophila, Caragana korshinskii and Hippophae rhamnoides, within two soil layers (0-20 cm and 30-50 cm) were compared. The results showed that S. psammophila had a higher leaf water potential than C. korshinskii and H. rhamnoides, the average maximum and minimum lumen diameter (d(max) and d(min), respectively), the average lumen area of vessels (Alum) and the ratio of lumen area of all vessels to xylem area (Aves/Axyl) in S. psammophila roots were also significantly higher than those in C. korshinskii and H. rhamnoides, and the root vessel density (VD) in S. psammophila was the same as that in H. rhamnoides but significantly higher than that in C. korshinskii. Root hydraulic conductivity in S. psammophila was 5 times of C. korshinskii and 2.8 times of H. hamnoides. The vulnerability index in S. psammophila roots was similar to that in C. korshinskii but higher than that in H. hamnoides. S. psammophila belonged to a water-spending species, whereas both C. korshinskii and H. rhamnoides were water-saving species, and C. korshinskii was more drought-resistant than H. rhamnoides. There was no difference of d(max), d(min) and Alum between roots in two soil layers, but roots within in the 30-50 cm soil layer had larger VD and Aves/Axyl. The root specific hydraulic conductivity within the 30-50 cm soil layer was significantly higher than within the surface soil layer, whereas the vulnerability index within the 30-50 cm soil layer was smaller, indicating roots in deep soil layers had higher hydraulic transport efficiency and lower hydraulic vulnerability.

  19. Current knowledge from experimental works with radioprotective drugs from the viewpoint of latest scientific research

    International Nuclear Information System (INIS)

    The protection of both human and animal population against a radiation impairment proves to be an impulse for continuous intensive searching for plants with radioprotective properties, identification of their radioprotective components and examination of their effects both in vivo an in vitro. I am presenting the results as well as knowledge of a latest scientific research in this field with testing the following plants: Vigna radiata, Mentha piperita, Citrus aurantium var. amara, Syzygium cumini, Tinospora cordifolia, Aegle marmelos, Phyllanthus amarus, Aloe vera, Angelica sinensis, Rosemarinus officinalis, Panax ginseng, Hippophae rhamnoides, Ocimum sanctum, Crataegus microphylla. (authors)

  20. Study on Biodiversity Index of Different Models of Conversion of Farmland to Forest in Huangshui River of Qinghai Province%青海湟水河流域不同退耕还林模式生物多样性研究

    Institute of Scientific and Technical Information of China (English)

    张学元

    2011-01-01

    基于香农-威纳多样性指教,研究了青海湟水河流域相同林龄(8年)的不同退耕还林模式生物多样性.结果表明,湟水河流域不同退耕还林模式生物多样性为云杉桦树混交>沙棘>桦树>云杉>云杉沙棘混交>农田,草本层多样性>乔木层.%Based on biodiversity index of Shannon-Weiner, the effects of biodiversity index from different models of conversion of farmland to forest in Huangshui river of Qinghai Province were studied, the results showed that mixture of picea crassifolia and birch > hippophae rham-noides > birch > picea crassifolia > mixture of picea crassifolia and hippophae rhamnoides > farmland, biodiversity index of herbaceous layer >arbor layer.

  1. Determination of the Macro Elements Content of Some Medicinal Herbs

    Directory of Open Access Journals (Sweden)

    Ducu Sandu Ştef

    2010-05-01

    Full Text Available The metals contents of plants are variable, due to the factors like differences between the plants species, geographical area, conditions of drying process. Metals contents in soil are a great importance for their effect of animals and humans, through the biologic chain: soil – plant – feed and food. Analysis of metals content was made with ContrAA-300, Analytik-Jena device, by flame atomic absorption spectrometry (FASS in air/acetylene flame. It were analyzed the macro elements content for 33 medicinal plants. The main macro elements (Ca, Mg K and Na were quantified for each sample. The contents in macroelements for analysed samples were in range: 3.763 % (Plantago major – 0.442 % (Hippophae rhamnoides, for Ca; 0.718% (Urtica dioica – 0.107% (Hippophae rhamnoides and Pinus, for Mg; 1.417% (Chelidonium majus – 0.319% (Rhamnus frangula, for K and 1.945% (Cynara scolymus – 0.021% (Pinus, for Na.

  2. Extraction and purification of the polysaccharides in Hippohaere rhamnoides L.

    Institute of Scientific and Technical Information of China (English)

    XU Yaqin; JIN Ting; YU Zeyuan; FU Hong

    2007-01-01

    The different extraction technology and purification technology of Hippohpae rhamoides polysaccharides were researched in the paper. The best method of papain extraction were obtained, the ratio of papain 2%, pH at 5.5, temperature at 45℃ and extraction time of 20 min were suitable for papain extraction. The highest content of Hippohpae rhamoides polysaccharides was 44.28 mg·g-1. The optimum process of ultrasonic extraction were obtained, namely extracted for 55 min at 480 W with the material ratio of 1:20. The highest content of Hippohpae rhamoides polysaccharides was 48.63 mg·g-1. The results showed that the ultrasonic and papain extraction together was the best method, the content was 54.30 mg·g-1. After the removing protein, pigment and dialysis. Two fraction were separated from the purified Hippohpae rhamoides by DEAE-cellulose chromatography, the main fraction was collected finally. The fraction was identified by Sepharose CL-4B gel filtration. Ultraviolet spectrometry, freeze-thawing analysis showed that fraction was purified. Its molecular weight was probably 109.4 ku.

  3. Radiation countermeasures from Himalayan herbs - an update

    International Nuclear Information System (INIS)

    A successful radioprotector is the one, which essentially renders protection from the multi-organ dis-function syndrome (MODS) caused by the total body exposure to ionizing radiation. Our rationale is that and instead of a single molecule, a group of molecules/compounds working synergistically can combat the MODS more effectively. Plant extracts offer natural combinations of a plethora of compounds, which act through different mechanisms and are, therefore, the ideal choice. Plants of Himalayan regions have survived under extreme climatic conditions through millions of years and are expected to harbor a battery of anti-stress adaptive molecules offering survival benefits. Our group is actively engaged in developing composite herbal radioprotective preparations from Himalayan Plants. This group is working to develop radiation countermeasures by tapping the essential complex phytochemicals from the plants inhabiting extreme climatic zones of Himalayas. In our laboratory, systematic studies were undertaken to investigate some of these plants located at high altitude regions of Himalayas viz. Hippophae rhamnoides, Rhodiola imbricata and Podophylium hexandruin. The most effective preparation from each of these plants individually, could provide more than 80% survival benefit to the irradiated (10 Gy) mice population against zero per cent survival in non-drug treated irradiated (10 Gy) mice population. It was observed that whole extracts of plant provided much better protection than the partial extracts/fractions. It was also observed that some of the partial extracts/fractions although, provided much higher survival benefits, yet were found to be unsuitable for drug development due to much higher mutagenic and/or recombinogenic effects in comparison to the whole extracts, One of the preparations from leaves of Hippophae rhamnoides (drug) showed more than 90% survival benefits in the irradiated mice population. Only one time intra-peritoneal administration of the drug

  4. Evaluation of nutrient value of seabuckthorn in north China

    Institute of Scientific and Technical Information of China (English)

    Hu Jian-zhong; Guo Xiao-feng

    2006-01-01

    Five minerals or elements, crude protein, lysine, methionine+cystine, calcium and phosphorus, were selected for evaluation of the food value of seabuckthorn (Hippophae rhamnoides ssp. sinensis) using the method of "food analysis". The decision values (δi)of seabuckthorn seeds, leaves and fruit residues rank in the fifth, sixth and ninth position among the 16 types of fodder. Seeds, leaves and fruit residues of seabuckthorn are suitable for livestock and poultry fodder. The weights of livestock and poultry are considerably increased after feeding with seabuckthorn. The development of seabuckthorn should have great potentials as food, pre-food and food additives for livestock and poultry in north China. Processing facilities should pay more attention to seabuckthorn food development methods and strategies and the seabuckthorn fodder industry should be promoted.

  5. Application in Supercritical Fluid Extraction of Effective Factor in Plant%超临界流体萃取在植物有效成分提取中的应用

    Institute of Scientific and Technical Information of China (English)

    刘艳

    2012-01-01

    The specificity of supercritical fluid,principle and schematic diagram of extraction are stated.The development in supercritical fluid extraction the effective factor in plan such as plan essential oil(rose essential oil,seed oil from hippophae rhamnoides),plan pigment(capsicum pigment,tomato red pigment) and factors in Chinese medicine(astragaloside,artemisinini) are reviewed.And the development of supercritical fluid extraction in future is forecasted.%文章论述了超临界流体的基本特性和萃取的原理,超临界流体在植物有效成分如植物精油、植物色素和中药有效成分等方面的研究现状,以及超临界流体萃取技术的发展趋势.

  6. Isorhamnetin Attenuates Staphylococcus aureus-Induced Lung Cell Injury by Inhibiting Alpha-Hemolysin Expression.

    Science.gov (United States)

    Jiang, Lanxiang; Li, Hongen; Wang, Laiying; Song, Zexin; Shi, Lei; Li, Wenhua; Deng, Xuming; Wang, Jianfeng

    2016-03-01

    Staphylococcus aureus, like other gram-positive pathogens, has evolved a large repertoire of virulence factors as a powerful weapon to subvert the host immune system, among which alpha-hemolysin (Hla), a secreted pore-forming cytotoxin, plays a preeminent role. We observed a concentration-dependent reduction in Hla production by S. aureus in the presence of sub-inhibitory concentrations of isorhamnetin, a flavonoid from the fruits of Hippophae rhamnoides L., which has little antibacterial activity. We further evaluate the effect of isorhamnetin on the transcription of the Hla-encoding gene hla and RNAIII, an effector molecule in the agr system. Isorhamnetin significantly down-regulated RNAIII expression and subsequently inhibited hla transcription. In a co-culture of S. aureus and lung cells, topical isorhamnetin treatment protected against S. aureus-induced cell injury. Isorhamnetin may represent a leading compound for the development of anti-virulence drugs against S. aureus infections. PMID:26643966

  7. Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae strain BIHB 723 from the cold deserts of the Himalayas.

    Science.gov (United States)

    Gulati, Arvind; Vyas, Pratibha; Rahi, Praveen; Kasana, Ramesh Chand

    2009-04-01

    A phosphate-solubilizing bacterial strain BIHB 723 isolated from the rhizosphere of Hippophae rhamnoides was identified as Acinetobacter rhizosphaerae on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, and 16S rRNA gene sequence. The strain exhibited the plant growth-promoting attributes of inorganic and organic phosphate solubilization, auxin production, 1-aminocyclopropane-1-carboxylate deaminase activity, ammonia generation, and siderophore production. A significant increase in the growth of pea, chickpea, maize, and barley was recorded for inoculations under controlled conditions. Field testing with the pea also showed a significant increment in plant growth and yield. The rifampicin mutant of the bacterial strain effectively colonized the pea rhizosphere without adversely affecting the resident microbial populations. PMID:19137371

  8. Sap flow in response to rainfall pulses for two shrub species in the semiarid Chinese Loess Plateau

    Directory of Open Access Journals (Sweden)

    Jian Shengqi

    2016-06-01

    Full Text Available Rainfall pulses can significantly drive the evolution of the structure and function of semiarid ecosystems, and understanding the mechanisms that underlie the response of semiarid plants to rainfall is the key to understanding the responses of semi–arid ecosystems to global climatic change. We measured sap flow in the branches and stems of shrubs (Caragana korshinskii Kom. and Hippophae rhamnoides Linn. using sap flow gauges, and studied the response of sap flow density to rainfall pulses using the “threshold–delay” model in the Chinese Loess Plateau. The results showed that the sap flow began about 1 h earlier, and increased twofold after rainfall, compared to its pre-rainfall value. The sap flow increased significantly with increasing rainfall classes, then gradually decreased. The response of sap flow was different among rainfall, species, position (branch and stem during the pulse period, and the interactive effects also differed significantly (P < 0.0001. The response pattern followed the threshold–delay model, with lower rainfall thresholds of 5.2, 5.5 mm and 0.7, 0.8 mm of stem and branch for C. korshinskii and H. rhamnoides, demonstrating the importance of small rainfall events for plant growth and survival in semi–arid regions.

  9. Contrasting water use pattern of introduced and native plants in an alpine desert ecosystem, Northeast Qinghai-Tibet Plateau, China.

    Science.gov (United States)

    Wu, Huawu; Li, Xiao-Yan; Jiang, Zhiyun; Chen, Huiying; Zhang, Cicheng; Xiao, Xiong

    2016-01-15

    Plant water use patterns reflect the complex interactions between different functional types and environmental conditions in water-limited ecosystems. However, the mechanisms underlying the water use patterns of plants in the alpine desert of the Qinghai-Tibet Plateau remain poorly understood. This study investigated seasonal variations in the water sources of herbs (Carex moorcroftii, Astragalus adsurgens) and shrubs (Artemisia oxycephala, Hippophae rhamnoides) using stable oxygen-18 isotope methods. The results indicated that the native herbs (C. moorcroftii, A. adsurgens) and one of the shrubs (A. oxycephala) mainly relied on water from the shallow layer (0-30 cm) throughout the growing season, while the introduced shrub (H. rhamnoides) showed plasticity in switching between water from shallow and deep soil layers depending on soil water availability. All studied plants primarily depended on water from shallow soil layers early in the season. The differences of water use patterns between the introduced and native plants are closely linked with the range of active root zones when competing for water. Our findings will facilitate the mechanistic understanding of plant-soil-water relations in alpine desert ecosystems and provide information for screening introduced species for sand fixation.

  10. Response pattern of amino compounds in phloem and xylem of trees to soil drought depends on drought intensity and root symbiosis.

    Science.gov (United States)

    Liu, X-P; Gong, C-M; Fan, Y-Y; Eiblmeier, M; Zhao, Z; Han, G; Rennenberg, H

    2013-01-01

    This study aimed to identify drought-mediated differences in amino nitrogen (N) composition and content of xylem and phloem in trees having different symbiotic N(2)-fixing bacteria. Under controlled water availability, 1-year-old seedlings of Robinia pseudoacacia (nodules with Rhizobium), Hippophae rhamnoides (symbiosis with Frankia) and Buddleja alternifolia (no such root symbiosis) were exposed to control, medium drought and severe drought, corresponding soil water content of 70-75%, 45-50% and 30-35% of field capacity, respectively. Composition and content of amino compounds in xylem sap and phloem exudates were analysed as a measure of N nutrition. Drought strongly reduced biomass accumulation in all species, but amino N content in xylem and phloem remained unaffected only in R. pseudoacacia. In H. rhamnoides and B. alternifolia, amino N in phloem remained constant, but increased in xylem of both species in response to drought. There were differences in composition of amino compounds in xylem and phloem of the three species in response to drought. Proline concentrations in long-distance transport pathways of all three species were very low, below the limit of detection in phloem of H. rhamnoides and in phloem and xylem of B. alternifolia. Apparently, drought-mediated changes in N composition were much more connected with species-specific changes in C:N ratios. Irrespective of soil water content, the two species with root symbioses did not show similar features for the different types of symbiosis, neither in N composition nor in N content. There was no immediate correlation between symbiotic N fixation and drought-mediated changes in amino N in the transport pathways.

  11. 青海湟水河流域不同退耕还林模式水文效应%Hydrological Effects of Different Sloping Land Conversion Program Models in Huangshui River Watershed of Qinghai Province

    Institute of Scientific and Technical Information of China (English)

    董旭

    2011-01-01

    基于野外采样、定点观测和室内实验,研究青海湟水河流域不同退耕还林模式水文效应,结果表明:不同林分类型枯落物有效持水性大小顺序为云杉桦树混交〉桦树〉云杉〉沙棘,其林下土壤持水性大小顺序为云杉桦树混交〉沙棘〉桦树〉云杉〉农田;森林土壤是森林生态系统水文效应的主体,枯落物和土壤总持水性大小顺序为云杉桦树混交〉沙棘〉桦树〉云杉〉农田。不同林分类型枯落物持水量决定于枯落物蓄积量和自然含水率,其林下土壤持水量决定于土壤非毛细管孔隙度。%Based on field investigation conversion program models in Huangsh and experimental data, hydrological effects from different sloping land ui river watershed of Qinghai province were studied, the results showed that : the descend order of different forest litter' s water holding capacity was as flows, mixture of picea crassifolia and birch, birch, pieea crassifolia, hippophae rhanmoides ; the descend order of different forest soil' s was as flows, mixture of picea crassifolia and birch, hippophae rhamnoides, birch, picea crassifolia, farmland ; forest soil was the principal part of forest ecosystem hydrological effects ;the descend order of forest litter and soilg to- tal water holding capacity was as flows, mixture of picea crassifolia and birch, hippophae rhamnoides, birch, picea crassifolia, farmland. Litter storage and its natural moisture content were the main reason of water holding capacity, non-capillary porosity of soil was the main reason of forest water holding capacity.

  12. The radioprotector properties of fruit new-perspective form of sea-buckthorn

    International Nuclear Information System (INIS)

    Full text : Recently in order to protect organisms from the radiation effect, radioprotectors have been applied principally concerning to sulfur compounds, aminothioles and indolilalkins. Although the achievements obtained in the direction of synthetic medicines reveal that they cannot completely replace the medicinal preparation of natural origin. Azerbaijan is considered as one of the richest countries for its flora species richness. The plants with essence oil are potential radio protectors. In the article it is intended to prepare aerosol, consisted of essence oils and lipid mixtures, for respiratory system treatment in extreme conditions having radiation threat

  13. Consumer-led development of novel sea-buckthorn based beverages

    DEFF Research Database (Denmark)

    Geertsen, Julie Leth; Allesen-Holm, Bodil Helene; Giacalone, Davide

    2016-01-01

    , the aims of the present study were to test how newly developed SBBs were perceived compared to a selected market reference, and to determine their acceptability with a representative sample of the Danish consumer population. Using a combination of consumer-friendly product profiling methods, we......, but were perceived as appropriate for fewer usages than the existing ones, suggesting that consumers may find it hard to envisage how to incorporate novel SBBs in their existing dietary habits. Consumer demographics and psychographics did not significantly affect product acceptance, indicating that sensory...... characteristics were the most important drivers of consumer preferences within this product category. Practical Applications This study offers new insights into consumers’ perception of novel food products of local origin. A key issue with such ingredients is that they have unique sensory properties which...

  14. 六盘山北侧叠叠沟小流域几种典型植被群落的水文功能研究%Hydrological functions of several vegetations in Diediegou valley in north Liupan Mountain

    Institute of Scientific and Technical Information of China (English)

    刘千; 王平; 熊伟; 王彦辉; 杜阿朋; 李振华; 王艳兵

    2013-01-01

      在宁夏六盘山北侧的半干旱区,研究了华北落叶松林、沙棘灌丛、虎榛子灌丛、草地植被类型的冠层截留与再分配、枯落物持水及土壤蓄水等水文功能。结果表明,不同植被类型的冠层截留能力相差较大,其中,沙棘灌丛的截留率最高(24.80%),华北落叶松林略低(21.40%),虎榛子灌丛最低(9.25%);与此相反,树干茎流率以虎榛子灌丛为最高(33.18%),超出大多数干旱半干旱区灌木树干茎流范围(2%~10%)。从季节变化上看,不同植被群落的截留率均为6月份最大,7—9月份持续下降,10月份出现回升;但截留量均为7月份最大。枯落物持水性能以华北落叶松林样地为最高,其次为虎榛子灌丛、沙棘灌丛和草地,这与各植被类型枯落物层贮存量的大小排序一致。1 m土层的土壤容重以虎榛子样地为最小(1.03 g・cm-3),然后依华北落叶松(1.07 g・cm-3)、草地(1.08 g・cm-3)、沙棘(1.16 g・cm-3)的顺序增大。土壤总孔隙度、最大持水量以华北落叶松林样地为最高,毛管孔隙度、毛管持水量以虎榛子样地为最高,非毛管孔隙度以草地为最高;这几项指标均以沙棘样地为最低。综上所述,从林冠截持、枯落物持水性、土壤物理性质等总体来看,华北落叶松人工林的土壤存蓄水、调水能力最强,其次为虎榛子灌丛和草地,沙棘灌丛最差。%The hydrological functions ,in terms of canopy interception and water holding capacity of litters and soil layers ,were assessed in several vegetations in the semi-arid region in north part of Liupan Mountain ,Ningxia ,China . The results indicated that the canopy interception differed notably among different vegetations ,with the maximal value (24 .80% ) in the shrubs of Hippophae rhamnoides ,followed by the plantations of Larix principis-rupprechtii (21

  15. Variations in seed germination of Hippophae salicifolia with different presoaking treatments

    Institute of Scientific and Technical Information of China (English)

    S. Airi; I.D. Bhatt; A. Bhatt; R.S. Rawal; U. Dhar

    2009-01-01

    Mature seeds of H. salicifolia, collected from five provenances (i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti) in west Himalaya, India, were treated with stratification (at 4oC for 15, 30 and 60 days) and in different concentrations of GA3 (5, 10, 20 mM), KNO3 (50, 100, 200 mM) and Thiourea (50, 100, 200 mM) solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control (24%-30%). The seeds treated with Thiourea (100 mM) have highest germination rate (76%-83% for different seed sources), followed by those (63%-71% for different seed sources) pretreated with stratification (4oC, 30 days). GA3 treatment significantly shortens the mean germination time (MGT) and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea (100 mM) and stratification (at 4oC) treatments for seed germination are recommended for mass multiplication through seeds of H. salicifolia in village/forest nurseries of the west Himalaya, India.

  16. Comparative analysis and nutritional composition of mulberry fruit morus alba plus seabuckthorn (hippophae) and their products

    International Nuclear Information System (INIS)

    The fruits of mulberry produced in Northern Pakistan were subjected to hot air dehydration to preserve without losing its natural flavour and nutrients. The dehydrated mulberry powder which is called mulberry beverage base (MBB) yielded good fruit tasty drink when mixed with suitable amount of water Shelf-life assessment was also conducted. The fresh fruit extract and the dehydrated mulberry beverage base (MBB) were analysed for juice/pulp, MBB, moisture, acidity as citric acid, total soluble solids, sugars, ascorbic acid and ash minerals like calcium, potassium, phosphorous, sodium and iron in fresh fruit extract was also determined. Mulberry pulp was mixed with seabuckthorn pulp prior to dehydration. Dehydrated product was found better in taste, colour and flavour. (author)

  17. Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Chao; LIU Guo-bin; XUE Sha; and XIAO Lie

    2013-01-01

    The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows:two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a signiifcant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions signiifcantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

  18. 沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响%Effects of sea buckthorn pulp oil and sea buckthorn seed oil on radiation skin lesions in rats

    Institute of Scientific and Technical Information of China (English)

    王养正; 吴英; Heikki Kallio; Yang Baoru; 王军宪; 王秉文; 崔刚

    2008-01-01

    目的 观察沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响.方法 实验用深部X线治疗机,每次以3000cGy的剂量,照射两次,造成大白鼠臀部放射性皮肤损伤.待动物症状明显后,将Ⅱ度皮肤损伤的动物随机分为6组,即阴性对照组、沙棘果油高剂量组、沙棘果油低剂量组、沙棘籽油高剂量组、沙棘籽油低剂量组以及阳性对照组.沙棘果油及籽油高剂量组,每天在损伤部位涂药两次,而其低剂量组每天涂药一次,阴性对照组每天涂灭菌的注射用水一次,阳性对照组每天涂Solcoseryl-Jelly软膏一次.结果 肉眼观察结果显示,连续用药10d后,沙棘果油、籽油及Solcoseryl-Jelly组动物的局部红斑、水肿明显减轻,创面分泌物明显减少,用药2周后,上述症状明显消退,与阴性对照组比较,明显好转;另外,沙棘果油、籽油及Solcoseryl-Jelly组的创面愈合时间明显缩短.光学显微镜观察结果显示,沙棘果油、籽油及Solcoseryl-Jelly组的皮肤损伤明显减轻,炎症反应亦明显减轻,创面修复明显.电子显微镜观察结果显示,阴性对照组的超微结构明显受损,而沙棘果油、籽油及Solcoseryl-Jelly组的损伤明显减轻.结论 沙棘果油及沙棘籽油对大鼠放射性皮肤损伤有明显的治疗作用.

  19. Histopathological alterations in the striatum caused by Karwinskia humboldtiana (Buckthorn) fruit in an experimental model of peripheral neuropathy.

    Science.gov (United States)

    Díaz-Pérez, Rosa Nelly; Castillo-González, Juan Antonio; Carcaño-Díaz, Katya; García-Juárez, Jaime; Salazar-Leal, Martha E; Muñoz-Maldonado, Gerardo E; Montes-de-Oca-Luna, Roberto; Saucedo-Cárdenas, Odila; Soto-Domínguez, Adolfo

    2016-04-01

    The accidental ingestion of Karwinskia humboldtiana (Kh) fruit in humans and animals causes chronic or acute intoxication. Acute poisoning induces respiratory failure that progresses rapidly to death. Studies in animals intoxicated with Kh describe lesions in cerebral cortex, cerebellum, spinal cord, hippocampus and caudate nucleus. Kh intoxication in Wistar rats models the sub-lethal clinical phase observed in humans. Considering these reports, the present study analyzed the histopathological alterations within the striatum following experimental Kh intoxication. Twenty Wistar rats were divided into three groups (n =5) and were intoxicated with Kh fruit. A control group (n =5) was included. Animals were euthanized at several time points (48, 58 and 170 days post-intoxication). The brain was collected, divided and processed for conventional histology or electron microscopy. Sections were stained with hematoxylin and eosin, cresyl violet, Klüver-Barrera, and toluidine blue. Immunolabeling was performed for glial cells in the striatum, and the samples were analyzed with light microscopy. Morphometric and statistical analyses were performed. In control group, neurons, axon bundles and neuropil had a normal appearance. At 48 days, hyperchromic neurons with apparent decreased size were observed interspersed among the normal neurons. At 58 days, we observed an increased number of hyperchromic neurons and disorganization of the myelin sheath and neuropil. At 170 days, these alterations persisted in the paralysis group. In treated groups, we observed signs of gliosis and increased axonal diameters. This study is the first report that describes the histopathological alterations within the striatum caused by chronic intoxication with Kh fruit in the Wistar rat. PMID:26544757

  20. Possibilities of cultivating ornamental trees and shrubs under conditions of air pollution with oxides of sulfur

    Energy Technology Data Exchange (ETDEWEB)

    Bialobok, S.; Bartkowiak, S.; Rachwal, L.

    1974-01-01

    The field work conducted has shown that high concentrations of SO/sub 2/ in the air can be withstood by the following trees and shrubs. Trees: Acer campestris, A. platanoides, Ailanthus altissima, Aesculus hippocastanum, Morus alba, Platanus acerifolia, Pinus strobur, P. nigra, Populus Berolinensis, P. candicans, P. Hybr. 27, P. Marilandica, P. simonii, P. Serotina, Quercus robus, Robinia pseudoacacia. Shrubs: Caragana arborescens, Crataegus oxyacantha, C. monogyna, Cerasus mehaleb, Forsythia/most of the species and varieties/, Ligustrum vulgare, Philadelphus coronaria, Ptelea trifoliata, Sambucus nigra, Salix caprea, Sorbaria sorbifolia, Sorbus aucuparia, Taxus baccata. For the selection of trees and shrubs in the laboratory, high concentrations of SO/sub 2/ were used (60-150 ppm for a period of 10 minutes). Experiments were conducted on cut shrubs kept in the gas chambers. In order to estimate the degree of their injury, they were transferred to a shaded greenhouse. A concentration of 65 ppm of SO/sub 2/ could be withstood by the following Forsythias: Forsythia intermedia Primulina, F. Densiflora, F. Spectabilis, F. giraldina, F. suspensa, F. koreana, F. ovata, F. japonica and Hippophae rhamnoides. A concentration of 130 ppm could be withstood only by F. intermedia Vitelina. A similarly high concentration of SO/sub 2/ could be withstood by shoots and leaves of Ailanthus girladii Duclouxii and by Platanus acerifolia. From among the lilacs Syringa pekinensis and S. amurensis proved resistant to high concentrations of SO/sub 2/.

  1. Optimized Extraction Technology of Flavonoids from Puree of Seabuckthorn Fruits%沙棘果泥中黄酮提取工艺优化研究

    Institute of Scientific and Technical Information of China (English)

    鲁长征; 山永凯; 刘明

    2012-01-01

    本试验主要研究沙棘果榨汁后的沉淀物中黄酮的最佳提取工艺。采用乙醇回流浸提的方法提取沙棘果泥中的黄酮,使用分光光度计对沙棘果泥中的黄酮含量进行测定。利用正交试验考察提取温度、提取时间以及料液比对黄酮提取率的影响,最终确定提取黄酮的最佳工艺。结果表明:最佳提取条件为提取温度75℃、提取时间2h、料液比1∶45。%The major objective of this experiment is to optimize the extraction technology of flavonoids from Hippophae rhamnoides puree after juicing. Prior to determination of the flavonoids content by spectrophotometer, ethanol refluxing was employed to isolate flavonoids. Orthogonal experiments were adopted to analyze the effect of each factor in extraction temperature, extraction time and solid- liquid ratio on extraction percentage of flavonoids to ascertain the optimized extraction technology.

  2. Impacts of mixed litter decomposition from Robinia pseudoacacia and other tree species on C loss and nutrient release in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    Xiaoxi Zhang; Zengwen Liu; Bochao Zhu; Yuanhao Bing; Nhu Trung Luc; Liangzhen Du; Zhenhua Zhu

    2016-01-01

    The productivity of Robinia pseudoacacia (R.p.) pure forest usually declines at the late growth stage, and reforming it into mixed forests could be a promising way to resolve this problem. When choosing a suitable tree species that can be mixed with R.p., the interspecific relationship is an important issue. Therefore, we gathered the autumn litter fall from R.p. and 10 other species from the Loess Plateau of China were mixed in dual species litterbags (R.p. ? each other species) and buried them in soil for a 345 days lab decay incubation. We measured the litter mass loss and nutrient contents to determine whether the nutrient release was affected by mixed species litter decomposition. The impacts of mixed litter decomposition on macro-elements release were more obvious than on micro-elements. The litters with similar substrate quality might show variable impacts on nutrients release in mixed decomposition. The C loss and release of nutrient was improved by descending order when R.p. litter was mixed with Hippophae rhamnoides, Ulmus pumila, Populus simonii, Larix principis-rupprechtii and Quercus liaotun-gensis (Q.l.). But, except for Q.l., only the other species were recommended as suitable mix-plants for R.p. since promoting a high turnover of the nutrient in the litter compartment and a rapid availability for tree.

  3. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity.

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Bin Asad, Muhammad Hassham Hassan; Akram, Muhammad Rouf; Khan, Abida Kalsoom; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed. PMID:26448818

  4. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Directory of Open Access Journals (Sweden)

    Saima Jadoon

    2015-01-01

    Full Text Available The exposure to ultraviolet radiations (UVR is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS, leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  5. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity.

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Bin Asad, Muhammad Hassham Hassan; Akram, Muhammad Rouf; Khan, Abida Kalsoom; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  6. Isolation and analysis of vitamin B12 from plant samples.

    Science.gov (United States)

    Nakos, M; Pepelanova, I; Beutel, S; Krings, U; Berger, R G; Scheper, T

    2017-02-01

    Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n=6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37μg/100g dry weight), in Elymus (26μg/100g dry weight) and in Inula helenium (11μg/100g dry weight). PMID:27596424

  7. Micromonospora is a normal occupant of actinorhizal nodules

    Indian Academy of Sciences (India)

    Lorena Carro; Petar Pujic; Martha E Trujillo; Phillipe Normand

    2013-11-01

    Actinorhizal plants have been found in eight genera belonging to three orders (Fagales, Rosales and Cucurbitales). These all bear root nodules inhabited by bacteria identified as the nitrogen-fixing actinobacterium Frankia. These nodules all have a peripheral cortex with enlarged cells filled with Frankia hyphae and vesicles. Isolation in pure culture has been notoriously difficult, due in a large part to the growth of fast-growing contaminants where, it was later found, Frankia was slow-growing. Many of these contaminants, which were later found to be Micromonospora, were obtained from Casuarina and Coriaria. Our study was aimed at determining if Micromonospora were also present in other actinorhizal plants. Nodules from Alnus glutinosa, Alnus viridis, Coriaria myrtifolia, Elaeagnus x ebbingei, Hippophae rhamnoides, Myrica gale and Morella pensylvanica were tested and were all found to contain Micromonospora isolates. These were found to belong to mainly three species: Micromonospora lupini, Micromonospora coriariae and Micromonospora saelicesensis. Micromonospora isolates were found to inhibit some Frankia strains and to be innocuous to other strains.

  8. An approach for detecting five typical vegetation types on the Chinese Loess Plateau using Landsat TM data.

    Science.gov (United States)

    Wang, Zhi-Jie; Jiao, Ju-Ying; Lei, Bo; Su, Yuan

    2015-09-01

    Remote sensing can provide large-scale spatial data for the detection of vegetation types. In this study, two shortwave infrared spectral bands (TM5 and TM7) and one visible spectral band (TM3) of Landsat 5 TM data were used to detect five typical vegetation types (communities dominated by Bothriochloa ischaemum, Artemisia gmelinii, Hippophae rhamnoides, Robinia pseudoacacia, and Quercus liaotungensis) using 270 field survey data in the Yanhe watershed on the Loess Plateau. The relationships between 200 field data points and their corresponding radiance reflectance were analyzed, and the equation termed the vegetation type index (VTI) was generated. The VTI values of five vegetation types were calculated, and the accuracy was tested using the remaining 70 field data points. The applicability of VTI was also tested by the distribution of vegetation type of two small watersheds in the Yanhe watershed and field sample data collected from other regions (Ziwuling Region, Huangling County, and Luochuan County) on the Loess Plateau. The results showed that the VTI can effectively detect the five vegetation types with an average accuracy exceeding 80 % and a representativeness above 85 %. As a new approach for monitoring vegetation types using remote sensing at a larger regional scale, VTI can play an important role in the assessment of vegetation restoration and in the investigation of the spatial distribution and community diversity of vegetation on the Loess Plateau.

  9. Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction.

    Directory of Open Access Journals (Sweden)

    Yun Luo

    Full Text Available Isorhamnetin (Iso is a flavonoid compound extracted from the Chinese herb Hippophae rhamnoides L. Previous studies have revealed its anti-cancer, anti-inflammatory, and anti-oxidant activities. This study investigated the ability of Iso to inhibit oxidized low-density lipoprotein (ox-LDL-induced cell apoptosis in THP-1-derived macrophages. The effects of Iso on atherosclerosis in vivo were also evaluated in apolipoprotein E knockout (ApoE-/- mice fed a high fat diet.Iso showed significant inhibitory effects on ox-LDL-induced THP-1-derived macrophage injuries via decreasing reactive oxygen species levels, lipid deposition, and caspase-3 activation, restoring mitochondrial membrane potential, reducing the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL-positive cells, and regulating apoptosis-related proteins. We also determined the protective effects of Iso by PI3K/AKT activation and HO-1 induction. Iso reduced the atherosclerotic plaque size in vivo in ApoE-/- mice as assessed by oil red O, Sudan IV staining, and CD68-positive cells, and reduced macrophage apoptosis as assessed by caspase-3 and TUNEL assays in lesions.In conclusion, our results show that Iso inhibited atherosclerotic plaque development in ApoE-/- mice by PI3K/AKT activation and HO-1 induction.

  10. ОПРЕДЕЛЕНИЕ ГАЛЛОВОЙ КИСЛОТЫ В МНОГОКОМПОНЕНТНЫХ РАСТИТЕЛЬНЫХ СРЕДСТВАХ МЕТОДОМ ВЭЖХ

    Directory of Open Access Journals (Sweden)

    Пунцык-Нима Базыровна Лубсандоржиева

    2014-01-01

    Full Text Available Определено содержание галловой кислоты в многокомпонентных сборах и эритрофите методом ВЭЖХ. Наибольшее количество галловой кислоты содержится в сборах, в состав которых входят листья Bergenia crassifolia (L. Fritsch., Urtica dioica L., Mentha piperita L., плоды Hippophae rhamnoides L.

  11. Role of the different planting age of seabuckthorn forests to soil amelioration in coal mining subsidence land

    Institute of Scientific and Technical Information of China (English)

    Yinli Bi; Yanxu Zhang

    2014-01-01

    To investigate the effects of seabuckthorn (Hippophae rhamnoides) on soil amelioration, using the space replacement method, soil physical and chemical indexes as well as the microorganism quantity and soil enzyme activities were analyzed. The results showed that:the soil bulk density of surface soil decreased and soil porosity and field capacity increased after afforestation with seabuckthorn. The plant was found to effectively reduce the soil pH, increase the soil conductivity, soil organic matters and available nutrients. Soil microorganism quantity, soil enzyme activities were both higher in 0–20 cm layer than in 20–40 cm layer. With the increase years of remediation with seabuckthorn, the quantity of soil microorganism and enzyme activities were increasing to a higher level 5 to 8 years later. Our study indicates that seabuckthorn can effectively improve soil physical and chemical properties, increase the quantity of soil microor-ganisms and enzyme activities, which is of great significance for the ecosystem restoration in mining areas.

  12. 沙棘果实成熟期一些生化成分的变化%The Changes of Some Biochemical Constituents along Seabuckthorn Fruit Ripening

    Institute of Scientific and Technical Information of China (English)

    M.antonelli; A.Raffo; N.Nardo; F.Paoletti; 何京亮; 王涛

    2009-01-01

    为了分析沙棘浆果成分的组成特点(Hippophae rhamnoides L.),研究人员进行了若干关于品种、原产地、成熟时间的研究(Gao等,2000;Kallio等1999;Tang和Tigerstedt,2001)。这些研究大部分集中在微量化合物组成成分的测定方面。已知该微量化合物因其抗氧化性有保护健康的作用(引自Gao等)。在这个意义上,研究人员发现沙棘浆果富含抗坏血酸、酚类化合物、生育酚类、类胡萝卜素等。尽管值得深入研究,但是据文献,一些如黄酮醇和类胡萝卜素的复合物类还没被广泛研究。(Hakkinen等,1999;Yang和Kallio,2002)。

  13. Diversity, distribution and vegetation assessment in the Jahlmanal watershed in cold desert of the Lahaul valley, north-western Himalaya, India

    Directory of Open Access Journals (Sweden)

    Rawat YS

    2010-05-01

    Full Text Available Relict vegetation in the Jahlmanal watershed is sparsely and scantily distributed on forest land. Farmers maintain tree species (Salix spp., Populus spp., Hippophae rhamnoides on the boundaries of terraced agriculture fields to meet the fodder and fuel wood requirements. Juniperus macropoda was found in relict forest patches in sparse and stunted conditions. The density of Salix fragilis was 3340 trees ha-1 under agroforestry and 5520 trees ha-1 under forestry. The density of J. macropoda was 4200 tree ha-1 in natural forest followed by 700 tree ha-1 in agroforestry and 160 tree ha-1 in managed forestry systems. The agroforesty system of the watershed consists of 30% trees, 35% small trees and 35% shrubs species. Shrubs contributed the highest density with 59.1% of the total, followed by density of trees (29.5% and small trees (11.3%. Trees contributed highest basal area in all the three systems, i.e., agroforestry, forestry and forest. The relict forest patches are subjected to deforestation due to anthropogenic pressure, hence in-situ and ex-situ conservation are required. Efforts are needed for plantation of ecologically suitable multipurpose tree species with indigenous species in the watershed. Technological interventions are also required to improve the quality and resistance against drought and climate change.

  14. HOST PLANTS AND CLIMATIC PREFERENCES OF THE INVASIVE SPECIES METCALFA PRUINOSA (SAY 1830 (HEMIPTERA: FLATIDAE IN SOME PLACES FROM SOUTHERN ROMANIA

    Directory of Open Access Journals (Sweden)

    Daniela Barbuceanu

    2015-12-01

    Full Text Available Observations carried out in May-September 2015 in two sites of Southern Romania reveal a rich spectrum of host plants for Metcalfa pruinosa, which consists of 204 species in 56 families. The species it is noticed on weeds and cultivated plants. The remarkable polyphagia of this species, the lack of natural enemies, and the climatic conditions of 2015 - warm and dry summer, had lead to an invasion of M. pruinosa, in the researched areas; the highest numerical abundances are noticed in shady habitats. Furthermore, on herbs, such as Levisticum officinale, Artemisia dracunculus, Ocimum basilicum, Mentha spp., usually avoided by pests, were observed colonies of the species. It is recorded high numerical abundance on fruit trees and shrubs: Hippophaë rhamnoides, Juglans regia, Prunus cerasus, Vitis vinifera, Rubus idaeus. The harmful effect occurs on apple trees Romus 1 variety as a result of the association with another pest of American origin, Eriosoma lanigerum, situation that favors the attack of the Erwinia amylovora bacteria, causing the collapse of the tree. It is found that altitudes higher than 200 m do not represent a limitative factor in the spreading of species, one of the investigated sites being located at 304 m altitude.

  15. Relationships between xylem embolism and eco-physiological indices in eight woody plants in sltu(Ⅱ):The relationship with photosynthetic eco-physiological indices

    Institute of Scientific and Technical Information of China (English)

    AN Feng; CAI Jing; JIANG Zaimin; ZHANG Yuanying; ZHAO Pingjuan; ZHANG Shuoxin

    2007-01-01

    The relationship between xylem embolism and eco-physiology indices (I.e.photosynthetic available radiation,temperature,relative humidity,photosynthetic rate,transpiration rate,stomatal conductance and water use efficiency) in eight tree species was investigated in situ.The species studied,Robinia pseudoacacia L.,Acer truncatum Bge.,Hippophae rhamnoides L.,Ulmus pumila L.,Pinus tabulaeformis Carr., Pinus bungeana Zucc.ex Endl.,Ligustrum lucidum Ait.,and Salix matsudana Koidz.f.pendula Schneid,grow well on the Xilin campus of Northwest A&F University.Results indicated that photosynthetic available radiation,air temperature and relative humidity can affect xylem embolism by daily adjustment of stomatal conductance,transpiration rate and water relations of a tree.Embolism was a common case in the daily growth of the plants,and there was some correlation between xylem embolism and photosynthetic rate,transpiration rate,stornatal conductance,and water use efficiency.Embolism may thus be an adaptive mechanism by some tree species to water stress.

  16. Hippophae leaf extract (SBL-1) countered radiation induced dysbiosis in jejunum of total body 60Cobalt gamma - irradiated mice

    International Nuclear Information System (INIS)

    Single dose of SBL-1 administered at the rate 30 mg/kg body weight (b.w.) 30 min prior to whole body 60Co-gamma-irradiation at lethal dose (10 Gy), rendered >90% survival in comparison to zero survival in the non-SBL-1 treated 60Co-gamma-irradiated (10 Gy) mice population (J Herbs Spices Med Plants, 2009; 15(2): 203-215). Present study investigated the effect of SBL-1 on jejunal microbiota in lethally irradiated mice. Study was performed with inbred Swiss albino Strain 'A' male mice (age 9 weeks) weighing 28±2 g. The animals were maintained under controlled environment at 26±2℃; 12 h light/dark cycle and offered standard animal food (Golden feed, Delhi) as well as tap water ad libitum. Metagenomic DNA was extracted, purified and quantified from jejunum of the mice. Universal primers (27f and 1492r) were used to amplify the 16S rRNA DNA from the metagenomic DNA. Amplicons were sequenced, vector contamination and chimeras were removed. The sequences (GenBank Accession No: KF681283 to KF681351) were taxonomically classified by using Sequence Match program, Ribosomal Database Project as well as by nucleotide-BLAST (E-value: 10, database: 16S rRNA gene sequences, Bacteria and Archea). Phylogenetic Tree was prepared using MEGA 5.2 package, using maximum likelihood algorithm after sequence alignment by MUSCLE. Thermus aquaticus was used as out-group to construct rooted tree. Branch stability was assessed by bootstrap analysis. Untreated animals and the animals treated with SBL-1 had 100% Lactobacillus; 60Co gamma-irradiated animals had 55% Cohaesibacter (Alphaproteobacteria); 27% Mycoplasma (Tenericutes) and only 18% Lactobacillus; animals treated with SBL-1 prior to irradiation had 89% Lactobacillus and 11% Clostridium. This study demonstrated that treatment with SBL-1 at radioprotective doses before total body irradiation with lethal dose (10 Gy) countered the jejunal dysbiosis. (author)

  17. Root Ecological Niche Index and Root Distribution Characteristics of Artificial Phytocommunities in Rehabilitated Fields

    Institute of Scientific and Technical Information of China (English)

    Hu Jianzhong; Zhen Jiali; Shen Jingyu

    2006-01-01

    In the implementation phase of the Conversion of Cropland to Forest and Grassland (CCFG) project in China,it is important,from a scientific point of view,to recognize phytocommunities' characteristics,species compatibility,and ecological function.The ecological niche that roots occupy,their abundance and distribution,and the factors that affect them must be acknowledged.Following the methodology of community ecology,the total root mass of a phytocommunity is measured as cubic volume.Root biomass,length,and the number of roots in every diameter class,for each soil layer and for each plant species,are regarded as observation variables.In the first instance therefore,a new method to calculate the root ecological niche index (REND is proposed,embracing the entire phytocommunity of plantations.Using the new method,the roots of pbytocommunities in Datong County,Qinghai Province (one of the counties selected for the national CCFG experiment),are dealt with in this paper.The results show that most of the vertical distributions of plant roots belong to the type wherein the roots are concentrated in the topsoil layer (0-20 cm),far more than those in the lower soil layers.The RENI of pbytocommunities is higher than that of pure stands or monocultures.The distribution of RENI by root diameter can be divided into four types:J-type,inverse J-type,recumbent S-type,and U-type.RENI is positively correlated with the wet biomass of aboveground level stems,branches,and plant leaves,and with the species richness of phytocommunities.Although the RENIs of plantations in rehabilitated fields are a little lower than those of natural forests,they are higher than those of cultivated crops.The RENIs of three community types (Picea crassifolia+Hippophae rhamnoides ssp.sinensis,H.rhamnoides ssp.sinensis,and P.crassifolia) in rehabilitated fields benefit greatly from the restoration project.The implementation of the CCFG project is important for the increase in RENI and the multiple functions of

  18. Land-Use Conversion Changes the Multifractal Features of Particle-Size Distribution on the Loess Plateau of China.

    Science.gov (United States)

    Sun, Caili; Liu, Guobin; Xue, Sha

    2016-01-01

    Analyzing the dynamics of soil particle-size distributions (PSDs), soil nutrients, and erodibility are very important for understanding the changes of soil structure and quality after long-term land-use conversion. We applied multifractal Rényi spectra (Dq) and singularity spectra (f(α)) to characterize PSDs 35 years after conversions from cropland to shrubland with Caragana microphylla (shrubland I), shrubland with Hippophae rhamnoides (shrubland II), forested land, and grassland on the Loess Plateau of China. Multifractal parameters (capacity dimension (D₀), entropy dimension (D₁), D₁/D₀, correlation dimension (D₂), and Hölder exponent of order zero (α₀)) were used to analyze the changes of PSDs. Dq and f(α) characterized the PSDs well and sensitively represented the changes in PSDs after conversion. All types of land-use conversion significantly improved the properties of the topsoil (0-10 cm), but the effect of shrubland I and even forested land decreased with depth. All types of land-use conversion significantly increased D₁ and D₂ in the topsoil, and D₁ and D₂ in the 10-50 cm layers of shrubland II, forested land, and grassland and D₁ in the 50-100 cm layers of shrubland II were significantly higher relative to the control. Both D₁ and D₂ were positively correlated with the contents of soil nutrients and fine particles and were negatively correlated with soil erosion, indicating that D₁ and D₂ were potential indices for quantifying changes in soil properties and erosion. In conclusion, all types of land-use conversion significantly improved the conditions of the topsoil, but conversion from cropland to shrubland II, forested land, and grassland, especially shrubland II and grassland, were more effective for improving soil conditions in deeper layers. PMID:27527201

  19. Protective role of plants against harmful radiation

    International Nuclear Information System (INIS)

    The rapid technological advancement has increased human exposure to ionizing radiations enormously. Ionizing radiations produces deleterious effects in the living organisms. Widespread use of radiation in diagnosis therapy, industry, energy sector and inadvertent exposure during air and space travel, nuclear accidents and nuclear terror attacks requires safeguard against human exposures. Lead shielding and other physical measures can be used in such situations but with difficulty to manage; thus pharmacological intervention could be the most prudent strategy to protect humans against the harmful effect of ionizing radiations. These pharmacological agents are radioprotectives; The development of radioprotective agents has been the subject of intense research in view of their potential for use within a radiation environment. However, no ideal, safe synthetic radio protectors are available to date, so the search for alternative sources including plants has been ongoing. In Ayurveda, the traditional Indian system of medicine, several plants have been used to treat free radical-mediated ailments and, therefore, it is logical to expect that such plants may also render some protection against radiation damage. This all is due to antioxidant enzymes, nitroxides, and melatonin, antiemetic, anti-inflammatory. haemopoitic and immunostimulant compounds. Some of the plants which are found to be radioprotective are Centella asiatica, Ginkgo biloba, Hippophae rhamnoides, Ocimum sanctum, Podophyllurn hexandrum, Tinospora cordifolia, Emblica officinalis, Phyllanthus amarus, etc. So there is an urgent need to identify and characterize the many of the plants in relation to the radioprotection. Besides these medicinal plants there are also some fruits and vegetables which are having good response against harmful radiations such as Kiwifruit Actinidia deliciosa (Actinidaceae), Cape Gooseberry Physalis peruviana (Solanaceae). They protect against the radiation-induced damage by

  20. Soil characterization and differential patterns of heavy metal accumulation in woody plants grown in coal gangue wastelands in Shaanxi, China.

    Science.gov (United States)

    Yakun, Shi; Xingmin, Mu; Kairong, Li; Hongbo, Shao

    2016-07-01

    Soil contamination by heavy metals in coal mine wastelands is a significant environmental issue in most developing countries. The purpose of this study is to evaluate contamination characteristics in the coal mine wastelands of Sanlidong coal mine, Tongchuan, China. To achieve this goal, we conducted field sampling work, followed by further analysis of the properties of soil contamination and accumulation characteristics in woody plants. At this site, the pH value ranged from 4.41 to 7.88, and the nutrient content of the soil rose gradually with the time after deposition due to the weathering effect improving the soil quality. Meanwhile, the levels of Cd, Cr, Cu, Ni, and Zn gradually decreased with the passage time. Generally, heavy metal contamination was found to be more serious in the discharge refuse area, with Cd contamination at moderate or heavy levels; Ni, Zn, and Cu contamination at light levels; and with no Cr contamination. The geoaccumulation index (I geo) was highest for Cd (2.38-3.14), followed by Ni, Zn, Cu, and Cr. Heavy metals accumulated on the lower slopes and spread to the surrounding areas via hydrodynamic effects and wind. According to transfer and enrichment coefficient analyses, Robinia pseudoacacia, Ulmus pumila, and Hippophae rhamnoides with considerable biomass could be used as pollution-resistant tree species for vegetation restoration. This study provided a theoretical basis for the restoration of the ecological environment in the mining area. This report described a link between heavy metal contamination of soils and growth dynamics of woody plants in China. PMID:27025220

  1. Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS

    Science.gov (United States)

    Suchal, Kapil; Bhatia, Jagriti; Malik, Salma; Malhotra, Rajiv Kumar; Gamad, Nanda; Goyal, Sameer; Nag, Tapas C.; Arya, Dharamvir S.; Ojha, Shreesh

    2016-01-01

    Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt–eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression. PMID:27445803

  2. 北票生态造林树种水分生理因子与立地因子分析%Water Physiological Factors and Site Factors in Species of Ecological Forestation in Beipiao Area

    Institute of Scientific and Technical Information of China (English)

    潘兴

    2015-01-01

    Water physiological factors and site factors of resistant species which are suitable to be planted in semiarid climate of Beipiao area were discussed.Cold resistance of species were determined.Water physiology and quantitative analysis of species were conducted.Result shows that the descending order of resistant species in this area is Ziz-iphusjujuba Mill.var.spinosa,Pinustabulaeformis,Pinussylvestris var.mongolica,Prunussibirica L,Cotinus coggygria ,Sabina chinensis ,Platycladus orientalis ,Lespedeza bicolor ,Amorpha fruticosa ,Robinia pseucdoaca-cia,Hippophae rhamnoides,Caragana microphylla,Populus xiaozhuanica.Multiple regression relationship be-tween average height & site conditions of Pinustabulaeformis based on mathematical calculation method of Quanti-tative theory Ⅰwas discussed ,which provides strong support for this region’s ecological forestation.%为了探讨适宜于北票半干旱气候地区的抗旱树种水分生理因子与立地因子,确定树种抗旱性强弱,进行了树种水分生理测定和数量化分析,结果表明,该地区的抗旱树种由强到弱的顺序为山枣、油松、樟子松、山杏、黄栌、桧柏、侧柏、胡枝子、紫穗槐、刺槐、沙棘、锦鸡儿和小钻杨。依据数量化理论Ⅰ数学计算方法,探讨了油松优势平均高与立地条件多元回归关系式,为这一地区的生态造林提供有力的支撑。

  3. Intra-herb pharmacokinetics interaction between quercetin and isorhamentin

    Institute of Scientific and Technical Information of China (English)

    Ke LAN; Jian-lin HE; Yang TIAN; Fei TAN; Xue-hua JIANG; Ling WANG; Li-ming YE

    2008-01-01

    Aim: Quercetin and isorhamnetin are common constituents of some herb extracts, such as extracts of gingko leaves and total flavones of Hippophae rhamnoides L. The intra-herb pharmacokinetics interactions between isorhamnetin and quercetin were investigated in the present study. Methods: Human MDR1 cDNA transfected MDCKII cells were used to validate whether isorhamnein interacted with P-gp. Caco-2 transport assays and a randomized, 3-way crossover pharmacokinetics study in rats were used to investigate the pharmacokinetics interactions. HPLC was used to determine cell transport samples. The total plasma concentrations of quercetinand isorhamnetin were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) by treatment with β-glucuronidase and sulfatase. Results: The permeability ratio (absorptive permeability/secretive permeability) of isorhamnetin across human MDR1 cDNA transfected MDCKII cells, Caco-2 cells and wild-type MDCKII cells are 0.25±0.02, 0.74±0.05, and 1.41±0.06, respectively. This result proved the role of P-gp in the cell efflux of isorhamnetin. While co-transporting with each other across Caco-2 cells monolayer, the permeability ratio of isorhamnetin and quercetin increased by 4.3 and 2.2 times. After coadministration with each other to rats,the Cmax, AUC0-72h, and AUC0-∞ of both isorhamnetin and quercetin significantly increased compared with single administration. Conclusion: The above results proved intra-herb pharmacokinetics interaction between quercetin and isorhamentin. P-gp might play an important role, whereas other drug efflux pumps, such as multi-drug resistance associate protein 2 and breast cancer resistance protein, might be involved. Accordingly, besides the drug-herb interactions, intra-herb interaction might be brought into view with the wide use of herbal-based remedies.

  4. Land-Use Conversion Changes the Multifractal Features of Particle-Size Distribution on the Loess Plateau of China.

    Science.gov (United States)

    Sun, Caili; Liu, Guobin; Xue, Sha

    2016-01-01

    Analyzing the dynamics of soil particle-size distributions (PSDs), soil nutrients, and erodibility are very important for understanding the changes of soil structure and quality after long-term land-use conversion. We applied multifractal Rényi spectra (Dq) and singularity spectra (f(α)) to characterize PSDs 35 years after conversions from cropland to shrubland with Caragana microphylla (shrubland I), shrubland with Hippophae rhamnoides (shrubland II), forested land, and grassland on the Loess Plateau of China. Multifractal parameters (capacity dimension (D₀), entropy dimension (D₁), D₁/D₀, correlation dimension (D₂), and Hölder exponent of order zero (α₀)) were used to analyze the changes of PSDs. Dq and f(α) characterized the PSDs well and sensitively represented the changes in PSDs after conversion. All types of land-use conversion significantly improved the properties of the topsoil (0-10 cm), but the effect of shrubland I and even forested land decreased with depth. All types of land-use conversion significantly increased D₁ and D₂ in the topsoil, and D₁ and D₂ in the 10-50 cm layers of shrubland II, forested land, and grassland and D₁ in the 50-100 cm layers of shrubland II were significantly higher relative to the control. Both D₁ and D₂ were positively correlated with the contents of soil nutrients and fine particles and were negatively correlated with soil erosion, indicating that D₁ and D₂ were potential indices for quantifying changes in soil properties and erosion. In conclusion, all types of land-use conversion significantly improved the conditions of the topsoil, but conversion from cropland to shrubland II, forested land, and grassland, especially shrubland II and grassland, were more effective for improving soil conditions in deeper layers.

  5. ФЛОРИСТИЧЕСКИЕ НАХОДКИ АДВЕНТИВНЫХ И РАРИТЕТНЫХ ВИДОВ РАСТЕНИЙ НА ЮГО-ЗАПАДЕ СРЕДНЕРУССКОЙ ВОЗВЫШЕННОСТИ

    OpenAIRE

    Курской, А.; Тохтарь, В.; Чернявских, В.

    2014-01-01

    В ходе проведенного авторами статьи флористического обследования в природных экотопах Белгородской области отмечено 4 новых для Белгородской обл. вида: Psysalis philadelphica Lam. (= Psysalis ixocarpa Brot. ex Hornem.), Solidago gigantea Ait., Hippophaё rhamnoides L., Lupinus polyphyllus Lindl., а также выявлено свыше 30 новых местообитаний инвазионных и раритетных видов, таких как: Aster salignus Willd., Echinocystis lobata (Mich.) Torr. et Gray., Impatiens glandulifera Royle, Impatiens parv...

  6. 沙地珍贵经济树种的筛选%Screening on Valuable Economic Species on Sandy Land

    Institute of Scientific and Technical Information of China (English)

    毕庆玲; 杜宏志; 王庆茹; 王新宇

    2015-01-01

    对吉林西部几种珍贵经济林树种以造林成活率、抗寒指数、抗旱能力为主要选择目标进行了筛选,以期为吉林西部沙地治理的生态经济模式提供优良的能形成特色产业的经济林新品种。结果表明:欧李1号、欧李2号、向海一号桑、同发一号桑、丰产型西伯利亚杏是最适宜吉林西部沙地栽培的能形成特色产业的优良树种;龙王帽、丰产型文冠果、沙棘较适合年最低温度不低于-37℃地区的沙地栽培,在吉林西部栽培时应加强苗期管理和造林当年的抚育管理。%Several valuable species of economic forest in western Jilin Province were screened by taking afforestation survival rate ,cold‐resistant index ,drought resistance as the main targets ,to provide new varieties of economic forest with excellent characteristics industry for ecological economic model for sand control in western Jilin Province .Re‐sult shows that Cerasus humilis ‘Yihao’ ,Cerasus humilis ‘Erhao’ ,Morus alba ‘Xianghaiyihao’ ,Morus alba‘Tongfayihao’ ,A rmeniaca sibirica are optimal for planting in western Jilin Province;Prunus armeniaca ,X an‐thoceras sorbifolia ,Hippophae rhamnoides are suitable to be planted on sandy land with annual minimum tempera‐ture being below -37℃ .

  7. Demarcation of Seabuckthorn Plantations in Three Northern Areas of China

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Seabuckthorn (Hippophae rhamnoides) planting areas in the three northern areas (north, northeast and northwest) of China are divided into five planting zones: the semi-humid forest prairie climate zone for ecological and economic types of seabuckthorn plantations in the southern part of the Loess Plateau; the semi-arid steppe climate zone for similar types of plantations in the central part of the Loess Plateau; the arid desert steppe climate zone for ecological type of seabuckthorn plantations in the northern part of the Loess Plateau; the semi-arid and semi-humid steppe climate zone again for ecological and economic types of plantations in northern Hebei and western Liaoning and the cold humid steppe climate zone for economic types of plantations in the northern part of northeast China. The aim of this demarcation is to avoid a random introduction of seabuckthorn. In each of the five zones,objectives should be set and suitable seabuckthorn species, subspecies and varieties should be planted according to site conditions,seed sources and methods of tree breeding. The cultivation centers, bases, stations, or units should be established and successful models of seedling and planting methods should be encouraged. The principle of matching trees with suitable site conditions and adjusting measures to local conditions should be practiced. From a strategic viewpoint of solving ecological and economic problems of seabuckthorn development in the three northern areas, every seabuckthorn center must have its own germplasm nursery, standard plantation for popularizing, excellent seed and seedling nurseries and sufficient afforestation areas for demonstration and propaganda purposes. These measures would improve the ecological environment and promote economic and social development in the three northern areas of China.

  8. HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas

    Directory of Open Access Journals (Sweden)

    Raluca Maria Pop

    2013-11-01

    Full Text Available . High performance liquid chromatography (HPLC with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: Taraxacum officinalis (1, Cynara scolimus (2, Silybum marianum (3, Hypericum perforatum (4,  Chelidonium majus (5, Lycopodium clavatum (6 and  Hippophae rhamnoides (7  leaves and fruits.  Two products (A and B were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol were analyzed using HPLC-UV for their phenolics’ fingerprint and composition. The qualitative (untargeted analysis and quantitative (targeted analysis results were further compared using Principal Component Analysis (PCA in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were trans-cinnamic, chlorogenic, caffeic and p-coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids. In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.

  9. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review.

    Science.gov (United States)

    Bahramsoltani, Roodabeh; Farzaei, Mohammad Hosein; Rahimi, Roja

    2014-09-01

    Burn wound healing is a complicated process including inflammation, re-epithelialization, granulation, neovascularization and wound contraction. Several biochemicals are involved in burn healing process including antioxidants, cytokines and liver and kidney damage biomarkers. Although several preparations are available for the management of burn wound, there is still a necessity of researching for efficacious medicine. The aim of the present study was to evaluate herbal preparations and their phytochemical constituents for burn wound management. For this purpose, electronic databases including Pubmed, Scirus, Scopus and Cochrane library were searched from 1966 to July 2013 for in vitro, in vivo or clinical studies which examined the effect of any herbal preparation on different types of burn wound. Only 3 human studies were found to include in this review. In contrast, there were 62 in vivo and in vitro studies that show the need for more clinical trials to prove the plant's potential to cure burn wound. Among single herbal preparations, Allium sativum, Aloe vera, Centella asiatica and Hippophae rhamnoides showed the best burn wound healing activity. Flavonoids, alkaloids, saponins and phenolic compounds were active constituents present in different herbs facilitating wound closure. Glycosides including madecassoside and asiaticoside and proteolytic enzymes were among the main active components. Phytochemicals represented positive activity at different stages of burn wound healing process by various mechanisms including antimicrobial, anti-inflammatory, antioxidant, collagen synthesis stimulation, cell proliferative and angiogenic effect. Overall, several herbal medicaments have shown marked activity in the management of wounds-especially burn wounds-and therefore can be considered as an alternative source of treatment. Furthermore, various natural compounds with verified burn-induced wound healing potential can be assumed as future natural drugs. PMID:24895176

  10. Seabuckthorn Pulp Oil Protects against Myocardial Ischemia-Reperfusion Injury in Rats through Activation of Akt/eNOS.

    Science.gov (United States)

    Suchal, Kapil; Bhatia, Jagriti; Malik, Salma; Malhotra, Rajiv Kumar; Gamad, Nanda; Goyal, Sameer; Nag, Tapas C; Arya, Dharamvir S; Ojha, Shreesh

    2016-01-01

    Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia-reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt-eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression. PMID:27445803

  11. Damage to pancreatic acinar cells and preservation of islets of Langerhans in a rat model of acute pancreatitis induced by Karwinskia humboldtiana (buckthorn).

    Science.gov (United States)

    Carcano-Diaz, Katya; Garcia-Garcia, Aracely; Segoviano-Ramirez, Juan Carlos; Rodriguez-Rocha, Humberto; Loera-Arias, Maria de Jesus; Garcia-Juarez, Jaime

    2016-09-01

    Karwinskia humboldtiana (Kh) is a poisonous plant that grows in some regions of the American continent. Consuming large amounts of Kh fruit results in acute intoxication leading to respiratory failure, culminating in death within days. There is evidence of histological damage to the lungs, liver, and kidneys following accidental and experimental Kh intoxication. To date, the microscopic effect of Kh consumption on the pancreas has not been described. We examined the early effects of Kh fruit on pancreatic tissue at different stages of acute intoxication in the Wistar rat. We found progressive damage confined to the exocrine pancreas, starting with a reduction in the number of zymogen granules, loss of acinar architecture, the presence of autophagy-like vesicles, apoptosis and inflammatory infiltrate. The pancreatic pathology culminated in damaged acini characterized by necrosis and edema, with a complete loss of lobular architecture. Interestingly, the morphology of the islets of Langerhans was conserved throughout our evaluations. Taken together, our results indicate the damage induced by a high dose of Kh fruit in the Wistar rat is consistent with an early acute necrotizing pancreatitis that exclusively affects the exocrine pancreas. Therefore, this system might be useful as an animal model to study the treatment of pancreatic diseases. More importantly, as the islets of Langerhans were preserved, the active compounds of Kh fruit could be utilized for the treatment of acinar pancreatic cancer. Further studies might provide insight into the severity of acute Kh intoxication in humans and influence the design of treatments for pancreatic diseases and acinar pancreatic cancer. PMID:26877198

  12. Tibet sea -buckthorn plant's multiplicity and use%西藏沙棘属植物的多样性及利用

    Institute of Scientific and Technical Information of China (English)

    索朗白珍; 央珍

    2005-01-01

    本文报道了西藏所产沙棘属植物的种类、分布以及物种形成的内外因素.在揭示沙棘植物遗传多样性的本质的同时也提出了经济生态学的概念.倡导环境保护、开发利用、可持续地发展资源优势.

  13. Characterization of Soil Microbial Community Function and Structure in Rhizosphere of Typical Tree Species and the Meaning for Environmental Indication in the Loess Plateau%黄土高原典型林木根际土壤微生物群落结构与功能特征及其环境指示意义

    Institute of Scientific and Technical Information of China (English)

    张海涵; 唐明; 陈辉

    2009-01-01

    Determination of the soil microbial community structure in rhizosphere of typical tree species in the Loess Plateau can be of great theoretical significance for correctly assessing the characteristics of soil ecological rehabilitation of the Loess Plateau. In this study, spore density analysis, microbial cultivation and BIOLOG were employed to evaluate the AMF spore density and soil microbial community diversity under four tree species with vesicular-arbuscular mycorrhizae in ecological rehabilitation area of the Loess Plateau, north Shaanxi Province. The results show that the different tree species differed significantly in both soil microbial number and microbial functional diversity, AMF spore density of Hippophae rhamnoides soil is 2.24 times than that of the Robinia pseudoacacia soil, and the rank as following order: Hippophae rhamnoides > Sophora viciifolia > Caragana mwrophjlla > Robinia pseudoacacia . The statistical significant are detected in the bacteria and actinomyces numbers, however, there is no statistical significance in fungi number among the treatments. The principle component analyses indicates that scatter of Caragana microphylla and Hippophae rhamnoides axe smaller than that of Sophora viciifolia and Robinia pseudoacacia, these results suggest that the soil community structure strongly varied among the different tree species. Numbers of carbon sources related to the first two components are 14 and 8. Correlation analysis shows that the AMF spore density appeared extremely significantly and positively correlated with the number of bacteria, and the metabolic of amino acids, amines and aromatic compounds, respectively . Moreover, AMF spore density positively correlated with the average well color development (AWCD) , nevertheless, no correlations are found among AMF spore density, carboxylic acids, carbohydrates and polymers. These results suggest that AMF spore density is shown to be an important environmental biology parameter used in

  14. Characteristics of rainfall interception for four typical shrubs in Qilian Mountain%祁连山典型灌丛降雨截留特征

    Institute of Scientific and Technical Information of China (English)

    刘章文; 陈仁升; 宋耀选; 韩春坛

    2012-01-01

    Rainfall intercepting by vegetation plays an important role affecting the water balance at local and catchment scale due to the control that vegetation canopy exert by modifying both evaporation and the redistribution of incident rainfall. Qilian Mountain is source regions of Heihe River, Shiyang River and Shule River inland river basin. In consideration of widespread shrubs which account for 68% of the whole forest area of Qilian Mountain, the research on rainfall interception process of shrubs for understanding the impact of rainfall characteristics on alpine shrubs and revealing the mechanism of hydrologic cycle and water resources with the impact of the shrub canopy, especially in the mountainous regions of an inland river basin, is very important and necessary. This paper taked the four typical alpine shrubs Potentilla Jruticosa, Salix cupularis, Hippophae rhamnoides, and Caragana jubata in Qilian Mountain as test objects, based on the field experimental data from June to October 2010, characteristics of rainfall interception and rainfall redistribution of four typical alpine shrubs in Qilian Mountain was investigated by permanent plot method, and impact of rainfall characteristics on rainfall redistribution of shrubs was analyzed by statistical method. The results indicated that the gross rainfall was 298. 6 mm during the experimental period. Rainfall was intercepted entirely by shrubs when rainfall is less than 2.1 mm, gross rainfall which observed throughfall and stemflow was 283. 1 mm. The amount of throughfall of P.fhdicosa, S. Cupularis, H. Rhamnoides and C.jubata was 175.8 mm, 179.8 mm, 148. Lmm, and 170.4 mm. Throughfall percentages of P. Fhuicosa, S. Cupularis, H. Rhamnoides and C.jubata was 62.0% , 63.5% 52.3% , and 60.2% , respectively. Stemflow was 9.5 mm, 9.1 mm, 22. 5 mm, and 11.8 mm for P.fruticosa, S. Cupularis, H. Rhamnoides, and C. Jubata, and averaged 3. 4% , 3. 2% , 8.0% , and 4.2% of the gross rainfall, respectively. Interception was 62

  15. Extracting technology of total flavonoid glycosides in fruit of Hippopphae rhamnoides%沙棘果中总黄酮苷类的提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    袁媛; 张浩; 郑苗

    2012-01-01

    OBJECTIVE To find out the best method of extracting total flavonoid glycosides in the berries of seabuckthorn. METHODS Ethanol concentration, extraction temperature, ratio of liquid to solid and extraction time were selected by using single factor experiment and orthogonal experiment. RESULTS The optimum extraction conditions were obtained as extraction temperature of 70 °C , ethanol concentration of 60% , extraction time of 0. 5 h for each time, 3 times in all, ratio of liquid to solid 1: 15. CONCLUSION Total flavonoid aglycones obtained by hydrolyzing was first used as an index in process optimization of extracting flavonoid glycosides. It' s necessary to study the total flavonoids in seabuckthorn further. Meanwhile, we should not only determine the content of total flavonoid aglycones before hydrolyzing, but also determine the content of total flavonoid aglycones after hydrolyzing.%目的 探讨沙棘果实中总黄酮苷类的最佳提取工艺.方法 采用单因素实验和正交实验结合的方法筛选,对乙醇浓度、提取温度、料液比、提取时间进行四因素三水平的正交试验.结果 最佳工艺条件为70℃下采用60%的乙醇浸提,固液比1∶15,回流提取(0.5 h×3).结论 首次将水解后的沙棘总黄酮苷元作为沙棘总黄酮提取工艺的评价指标,在分析检测方法上不仅应测定水解前,还应考察测定水解后总黄酮苷元的含量.

  16. Trial-process on Stirred Yoghurt with Hippohas Rhamnoides L%沙棘搅拌型酸奶的研制

    Institute of Scientific and Technical Information of China (English)

    苏宇静

    2000-01-01

    论述了沙棘搅拌型酸奶的加工工艺,以沙棘汁、牛乳为原料,经乳酸发酵后生产出一种酸甜可口、风味独特、色泽宜人、营养丰富、老少皆宜的保健饮料.该产品具有很高的营养价值和独特的风味,是一个新的酸奶品种.

  17. Study on availability of soil phosphorus and plants adaptability in the Loess Hilly Region%黄土丘陵区土壤磷有效性与植物适应性研究

    Institute of Scientific and Technical Information of China (English)

    王恒威; 许明祥; 王爱国; 马昕昕

    2012-01-01

    communities in loess hilly region.The objective was to explore the effect of vegetation restoration on soil phosphorus availability,and to understand the suitability of vegetation communities to soil phosphorus condition.【Method】 Different plants of the 125 plots in Zhifanggou watershed of loess hilly region were selected,including Artemisia capillaris,Stipa bungeana,Artemisia gmelinii,Caragana intermedia,Hippophae rhamnoides,Robinia pseudocacia,taking the crop land as the control.Leaf and surface soil(0-20 cm) samples were collected,the soil total P,available P,pH and leaf P content were determined.The characteristics of soil and plant P nutrient,the relativity among soil P,soil pH and leaf P in different vegetation communities were analyzed.【Result】 The results showed a great shortage of soil available P content(1.54 mg/kg) and a relatively high content of soil total P in the loess hilly region.Significant difference was observed in soil available P content and soil pH among different plants,which was not observed in soil total P content.The soil available P in proper order was crop land〉Artemisia capillaris〉Hippophae rhamnoides,Robinia pseudocacia,Caragana intermedia〉Stipa bungeana,Artemisia gmelinii.The average leaf P content was 1.43 mg/g and the leaf P content of Artemisia capillaris,Hippophae rhamnoides was significantly higher than other plants(P〈0.05).There was no obvious correlation among soil available P content,soil pH and leaf P content in different plants(except Hippophae rhamnoides).【Conclusion】 Leaf P content and soil available P content in studied area were lower than the average value in the world.Non-significant correlation was found between P content of soil and plant leaf.The ratio of soil available P content in soil total P content increased with vegetation succession progress,suggesting that soil P status was also enhanced,artificial forest communities appeared to be higher demand of soil available P content than

  18. Effects of Biological Soil Crusts on Seed Emergence and Seedling Growth in Loess Plateau,North Shaanxi Province%黄土高原土壤生物结皮对植物种子出苗和生长的影响

    Institute of Scientific and Technical Information of China (English)

    王蕊; 朱清科; 赵磊磊; 常存; 马浩

    2011-01-01

    Biological soil crusts are prevalent in arid and semiarid regions,they are the complex mixtures of cyanobacteria,green algae,phycolichens,mosses,liverworts,fungi and bacteria.In order to understand the effect of biological soil crusts(BSCs) on seed emergence and seedling growth in loess region,we conducted field surveys and studied the impact of intact crusts,broken crusts and soil on seed emergence of 4 plant species including Pinus tabulaeformis,Caragana korshinskii,Hippophae rhamnoides and Pyrus betulifolia based on the field emergence experiment in Wuqi County,Shaanxi Province during the period from July to August 2009.The results reveal that the vegetation coverage was reduced significantly with the increase of BSCs coverage and thickness(P0.01),and vegetation types were in an increase trend(P0.05).Seed emergence rate and emergence process were affected by plant species and crust types(intact crusts,broken crusts and soil)(P0.01),and the interaction between the two affected slightly the seedling emergence(P0.05),but affected significantly the emergence process(P0.01).The seed emergence rate under broken BSCs was higher than that under intact BSCs,but there was no significant difference with soil.Broken BSCs could increase plant biomass and root-shoot ratio of plants,but intact BSCs affected the plant biomass and root-shoot ratio depending on the types of seed.%为了解生物土壤结皮对植物种子萌发出苗和植物生长的影响,于2009年7~8月在陕西省吴起县进行野外调查,对油松(Pinus tabulaeformis)、柠条(Caragana korshinskii)、沙棘(Hippophae rhamnoides)和杜梨(Pyrus betulae-folia)种子进行萌发实验,研究生物结皮与植被之间的相互关系以及完整结皮、破碎结皮和覆土处理对这4种种子出苗和生长的影响。结果表明:随着生物结皮盖度和厚度的增加,植被盖度和数量显著减少(P〈0.01),植被种类有增加的趋势(P〉0

  19. Influence of Groundwater Level on Community Characteristics of Liuwanlin Ecosystem in Mu Us Sandy Land%水位梯度对毛乌素沙地柳湾林群落特征影响

    Institute of Scientific and Technical Information of China (English)

    张琳; 杨劼; 呼格吉勒图; 宋炳煜; 丁越岿

    2012-01-01

    Based on the field survey data of typical plots and quadrats, the thesis researched the change of community types, species diversity and biomass of Liuwanlin ecosystem in Mu Us Sandy Land with cluster a- nalysis method. Results indicate that as the groundwater level reduces, the community components of Li- uwanlin ecosystem transit from Salixmicrostachya + Hippophae rhamnoides to Salix psammophila, and the Simpson index, Shannon-Wiener index, Margalef species abundance index, Pielou evenness index and biomass of community components of Liuwanlin ecosystem all present an obviously decreasing trend, and the correlation coefficients between the indices and the groundwater level are 0. 837, 0. 784, 0. 617, 0. 615, and 0. 710, respectively. So groundwater level is one of the important factors which influence species biodiversi- ty and productivity of the Liuwanlin ecosystem in the Mu Us Sandy Land.%根据典型样地和样方调查资料,采用聚类分析法,对毛乌素沙地柳湾林群落类型、多样性及生物量变化特征进行研究。结果表明,随着水位梯度的降低,柳湾林群丛类型由乌柳+中国沙棘群丛向北沙柳群丛过渡;辛普森指数、香浓一威纳指数、物种丰富度、均匀度指数和生物量均呈明显下降的趋势,相关系数分别为0.837、0.784、0.617、0.615、0.710。这表明水位梯度是影响毛乌素沙地柳湾林生态系统生物多样性与生产力的主要因素之一。

  20. The influence of some phytobiotics (thyme, seabuckthorn on growth performance of stellate sturgeon (A. stellatus, Pallas, 1771 in an industrial recirculating aquaculture system

    Directory of Open Access Journals (Sweden)

    Oana Georgiana Dorojan

    2014-05-01

    Full Text Available Obiectivul acestui experiment a fost de a evalua influența unor phytobiotics asupra performanțelor de creștere a sturionilor stelat ( A. stellatus . Au existat două variante experimentale: V1 - cimbru și V2 - catina. Peștele utilizat în acest experiment au fost de un an si patru luni, cu o greutate inițială medie de 122.78 ± 31.60 g / pește (V1, respectiv 121.21 ± 32,54 g / pește (V2. Cele phytobiotics folosite, cimbru ( Thymus vulgaris și cătină ( Hippophae rhamnoides , au fost încorporate în furaje, utilizând gelatină, având o concentrație de 2% / kg furaj. Alterna Storioni hrănesc - a fost folosit de 48% proteine ​​brute. Parametrii de calitate a apei în timpul experimentului au fost în limite normale pentru o creștere optimă a peștilor experimentale. La sfârșitul experimentului diferențele semnificative (p <0,05 au fost observate între cele două variante experimentale în termeni de greutate medie finală și rata de creștere individuală. Diferențele cu privire la SGR, FCR, PER au fost nesemnificative (p> 0,05. Valoarea cea mai mare rata de creștere individuală (105.90 g a fost înregistrată în cazul V2 și cea mai mică valoare (98,45 g în caz de V1. Ca o concluzie, se poate spune că cele două tipuri de phytobiotics (cimbru, cătină, administrate într-o concentrație de 2% / kg de furaje, au influențat performanța de creștere a sturionilor stelat.  

  1. On the identification of the original plants in the modernization of Chinese herbal medicine-An example from the taxonomy and exploitation of ‘Gouqi'%关于中药现代化中的物种鉴定问题 --基于枸杞分类和生产问题的讨论

    Institute of Scientific and Technical Information of China (English)

    路安民; 王美林

    2003-01-01

    在中药开发利用中,原植物的正确鉴定和命名是首先要解决的问题.以枸杞为例,考证了在古籍中曾发生过的枸杞属(Lycium)与白棘(酸枣Zizyphus jujuba Mill. var. spinose (Bunge) Hu)和沙棘(Hippophae rhamnoides L.)、藏药中枸杞与忍冬(Lonicera myrtillus Hook. f. et Thoms., L.microphylla Willd. ex Roem. et Schult.)和白刺(Nitraria tangutorum Bobr.)之间的混淆情况.通过对中国枸杞属种质资源的种类和分布介绍,详细比较了我国传统医药广泛利用的两种枸杞--枸杞(L. chinense Mill)和宁夏枸杞(L. barbarum L.)在形态上的区分,澄清了长期以来在分类上的混乱.通过对枸杞属在茄科植物中系统关系的综合分析,认为枸杞属同富含托品生物碱(tropane alkaloids)的天仙子类和曼陀罗类的关系甚远,它们分别隶属不同的族.对Harsh (1989)报道印度产的"L. barbarum"富含托品生物碱的原植物提出置疑.根据国产枸杞属植物的不同生物学特性,提出用现代技术加速选育优质及抗病虫害品种的紧迫性.文章结合枸杞子(Gouqizi, Fructus Lycii)的生产情况,提出建立和发展传统药材规范化生产基地的必要性.

  2. 中国沙棘果实化学成分的研究

    Institute of Scientific and Technical Information of China (English)

    郑瑞霞; 杨峻山

    2006-01-01

    中国沙棘Hippophae rhamnoides L.subsp.sinens isRousi为胡颓子科沙棘属的灌木或小乔木,又名醋柳。主要分布在欧亚大陆东经2°~115°,北纬27°~68.5°的广大地区。产于我国北部、西部、西南地区。沙棘性温、味酸涩,入肝、胃、大肠经,有活血散瘀、化痰宽胸、补脾健胃等功能。用于治疗跌打损伤、瘀血肿痛、胃痛、呼吸不畅、食欲不振等。原为我国的藏、蒙医常用药,1977年卫生部首次将该药正式收载于《中国药典》。对中国沙棘果实的化学成分的研究报道较少。为了充分利用药用资源,寻找新的活性成分,笔者对内蒙产的中国沙棘进行了系统研究。本实验从其果实中分离得到8个化合物,分别鉴定为:异鼠李素-7-O-鼠李糖-3-O-葡萄糖苷(isorham—netin-7-O—rhamnose-3-O-glucoside,Ⅰ)、异鼠李素(isorhamnetin,Ⅱ)、槲皮素(queretin,Ⅲ)、山柰酚(kaempferol,Ⅳ)、芹菜素(apigenin,Ⅴ)、2α-羟基乌苏酸(2a—hydroxyursolic acid,Ⅵ)、β谷甾醇(psitosterol,Ⅶ)及胡萝卜苷(daucosterol,Ⅶ),化合物Ⅰ和Ⅴ~Ⅶ均为首次从该果实中分得。

  3. The Anti-oxidative Effect of Sea Buckthorn Seed Procyanidins in Vitro%沙棘籽原花青素体外抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    徐晓云; 潘思轶; 谢笔钧; 曹少谦

    2005-01-01

    研究沙棘籽原花青素(SBSPC)体外抗氧化的作用.采用水杨酸法检测沙棘籽原花青素对羟自由基(·OH)的清除作用,用硫代巴比妥酸法测定小鼠肝丙二醛(MDA)的含量,用分光光度法测定小鼠红细胞溶血和肝线粒体肿胀程度,研究SBSPC的抗氧化效果.结果表明:SBSPC可以清除·OH,抑制·OH所致的丙二醛的产生,减少·OH所致的红细胞溶血,降低·OH所致线粒体肿胀程度,证明SBSPC具有抗·OH所致的氧化的作用.

  4. Antibacterial Activity in vivo and in vitro of Enzymatic Hydrolysates of Sea Buckthorn Seed Residue%沙棘籽渣酶解产物的体内外抑菌作用

    Institute of Scientific and Technical Information of China (English)

    陈彤; 王常青; 连伟帅; 宁庆鹏; 白云云; 李小凡; 郝志萍

    2015-01-01

    以沙棘籽渣为原料,采用水提法提取沙棘籽渣蛋白,再用ProteAX复合蛋白酶和胃蛋白酶两种酶酶解,利用膜过滤对其分离纯化,得到具有抑菌活性的沙棘籽渣蛋白酶解物.体外实验结果表明:ProteAX复合蛋白酶水解多肽对金黄色葡萄球菌、沙门氏菌、绿脓杆菌均有抑制作用,胃蛋白酶水解多肽对大肠杆菌、金黄色葡萄球菌、沙门氏菌有抑制作用.动物实验表明:胃蛋白酶水解多肽灌胃的小鼠粪便中,沙门氏菌的菌落总数与阳性对照组相比显著减少(P<0.05);而灌胃ProteAX复合蛋白酶水解多肽的小鼠粪便中,金黄色葡萄球菌的菌落总数比阳性对照组显著降低(P<0.05).

  5. 反相高效液相色谱法测定沙棘油中维生素E的含量%CONTENT DETERMINATION OF VITAMIN E IN OIL OF FRUCTUS HIPPOPHAE BY REVERSED PHASE-HIGH PERFORMANCE LIQUID CROMATOGRAPHY

    Institute of Scientific and Technical Information of China (English)

    吴虹; 吴健

    2002-01-01

    目的:采用反相高效液相色谱法测定沙棘油中维生素E的含量.方法:C18-ODS柱为色谱柱、甲醇-水(98∶2)为流动相,流速为1.5 ml/min,检测波长:280 nm.结果:线性范围0.9~6.0 μg(r=0.999 8),平均回收率为99.17%,RSD为1.48%(n=5).结论:本法操作简便,准确可靠.

  6. Root structure of slope protection plants in a high-grade highway

    Institute of Scientific and Technical Information of China (English)

    WANG Jing-hong; LI Xing-tao; LIU Xiao-dong; Wang Fei; LIU Yu

    2011-01-01

    Root length and root length density of Lespedeza bicolor,Amorpha fruticosa, and Sea buckthorn were investigated in a country highway-TongSan highway (Tongjiang to Sanya) in Heilongjiang Prov ince, China. The root lengths were divided into five root orders according to Pregizter sequence classification method. Results show that sea buckthorn roots are dominated by coarse roots in the horizontal growth, while L. bicolor has a large proportion of fine roots in vertical conical growth and A. fruticosa is in depth growth. Root length density of L. bicolor in all the root sequences is higher than that of sea buckthorn and A. fruticosa. On the basis of the root structure, it is inferred that L. bicolor roots mainly absorb the surface soil moisture for its normal growth; in contrast,A. fruticosa has good uptake ability to deep soil water. The root structure of sea buckthorn implies that it has a strong drought resistance.

  7. Drug: D06734 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available buckthorn family) Jujube seed Major component: Zizybeoside [CPD:C17564 C17565] Therapeutic category of drugs... in Japan [BR:br08301] 5 Crude drugs and Chinese medicine formulations 51 Crude drugs 510 Crude drugs 5100 Crude drugs...08304] Crude Drugs Drugs for Qi Sedative drugs D06734 Jujube seed Crude drugs [BR:br08305] Dicot plants: rosids Rhamnaceae (buckthorn family) D06734 Jujube seed PubChem: 47208385 ...

  8. Environ: E00447 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00447 Hovenia dulcis fruit or seed Hoveniae semen seu fructus Crude drug Sucrose [...CPD:C00089], D-Glucose [CPD:C00031], Fructose [CPD:C01496] Hovenia dulcis [TAX:99292] Rhamnaceae (buckthorn family) Hoven...ia dulcis mature fruit or seed (dried) Crude drugs [BR:br08305] Dicot plants: rosids Rhamnaceae (buckthorn family) E00447 Hovenia dulcis fruit or seed ...

  9. Studying possibility of using fruit powders in technology of sponge-cake semi-finished products

    OpenAIRE

    Доценко, Виктор Федорович; Мирошник, Юлия Анатольевна; Шидловская, Елена Брониславовна; Медвидь, Ирина Николаевна

    2014-01-01

    The paper shows the possibility of enriching flour confectionery with powders of viburnum, ashberry and sea buckthorn for producing sponge-cake semi-finished products, as fresh fruit production is a seasonal product and does not provide a regular supply of biologically active substances into the population food ration.The influence of viburnum, ashberry and buckthorn powders on the amount and quality of gluten in dough was studied. In addition, the appropriateness of using SAS (surface-active...

  10. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    purple willow (Salix purpurea). Only the 25% of the interventions was accomplished by the use of secondary plant species, as tamarisk (Tamarix spp.,) blackthorn (Prunus spinosa) , whitethorn (Crataegus spp.), sea-buckthorn (Hipphopae rhamnoides), wild pear (Pyrus pyraster), cottonwood (Populus nigra), eglantine (Rosa spp.), goat-willow (Salix caprea) and cornel (Cornus sanguinea). Better results were achieved with Spanish Broom, a very rural plant that can effectively colonise even poor soils like badlands; as a matter of fact, more than the 75% of the interventions had positive outcomes The efficacy of the consolidation work by the presence of living structures point out an increase of the stability of those interventions older than 4 years, with taking root species present from 54% to 78%. So far, the construction and the reliability of the works have been monitored, in order to capture critical aspects for the success of works and to build a geo-referenced data base of the existing works and their status.

  11. Community Diversity of Bacteria and Arbuscular Mycorrhizal Fungi in the Rhizosphere of Eight Plants in Liudaogou Watershed on the Loess Plateau China%黄土高原六道沟流域8种植物根际细菌与AMF群落多样性研究

    Institute of Scientific and Technical Information of China (English)

    封晔; 唐明; 陈辉; 丛伟

    2012-01-01

    Hippophae rhamnoides (1.21). Cluster analysis and redundancy analysis indicated a significant difference in associated microbial community structure, while the similarity among community diversity of rhizospheric bacteria and AMF associated with specific plants was also found. There was a significant correlation between diversity indices of bacteria and AMF (P〈0.01). Associated microbial community diversity was influenced primarily by organic matter and total nitrogen content. Our work demonstrated strong impacts of plant species and rhizospheric environment on associated microbial community structure. Due to the high diversity indices of rhizospheric bacteria and AMF, R. pseudoacacia was considered to be a pioneer plant species for vegetation restoration in the Liudaogou watershed.

  12. Restoration techniques of degraded swamp in Xiaoxing'an Mountains, northeastern China%小兴安岭退化沼泽湿地植被恢复技术

    Institute of Scientific and Technical Information of China (English)

    张功宝; 蔡体久; 徐飞

    2014-01-01

    In this study, three types of disturbed ( fire, grazing and reclamation ) degraded swamps were investigated in Youhao National Nature Reserve. To analyze the recovery effect of different planting systems applied to degraded swamps, plot investigation method and excavation method were used to investigate plant growth index. The results showed that: in fire disturbed swamp, pure spruce ( Picea koraiensis) plantation was the best one among seven planting systems, the survival rate of tree was 89% 3 years after planting, and quite low in other planting systems. In grazing disturbed swamp, the recovery effect of the afforestation with either pure spruce or pure larch ( Larix gmelinii) or mixed larch and spruce forest was satisfactory, and the survival rates of tree were 94%, 79% and 85. 5% respectively. In agricultural reclamation disturbed swamp, the recovery effect of the afforestation with either pure spruce or pure larch or mixed larch and spruce forest was similar to the grazing discurbed swamps with survival rates 92%, 85% and 83% respectively. Seabuckthorn (Hippophae rhamnoides) had lowest survival rates (<36%) in all these disturbed swamps, it is not a suitable species for vegetation recovery in the degraded swamps in this area. Spruce (P. koraiensis) and larch (L. gmelinii) are the recommended species for vegetation recovery in the degraded swamps in Xiaoxing’an Mountains of northeastern China.%以黑龙江省友好国家级自然保护区火烧、放牧和垦殖3种干扰类型退化森林沼泽湿地为研究对象,应用样地调查法和挖掘法对植物生长指标进行调查,分析不同栽植模式对退化沼泽湿地植被的恢复效果。结果表明:在火烧干扰湿地中,栽植红皮云杉纯林是最好的植被恢复模式,栽植3年成活率为89%,其他植被恢复模式的树木成活率都很低。在放牧干扰湿地中,红皮云杉纯林、落叶松纯林模式和红皮云杉与落叶松混交林的造林模式恢复效果好,3

  13. 核磁共振法与索氏提取法测定能源植物种子含油量的比较研究%Comparative study on determination of seed oil content of energy plants by using NMR and Soxhlet Extraction

    Institute of Scientific and Technical Information of China (English)

    董晓丽; 白鹏莉; 王金妹; 阮成江

    2011-01-01

    The seed oil content is an important index for evaluating energy plants. To find a quick and precise method for testing the seed oil content, we measured the seed oil contents of four potential energy plants by using the methods of Nuclear Magnetic Resonance (NMR) and Soxhlet Extraction, and compared the results among them. Results showed that the seed oil contents tested by NMR are significantly higher than that of Soxhlet Extraction. For the two methods,the measured seed oil contents of Datura stramonium were 28.87% and 25.91%, respectively;17.74% and 14.78% for Kosteletzkya virginiea, respectively; 9.53% and 6.82% for Hippophae rhamnoides ssp.sinensis, respectively; and 47.01% and 44.31% for Ricinus communis, respectively. The data indicated that NMR method had the advantages of simple and damage free, which avoided the disadvantages of Soxhlet Extraction such as time-consuming and difficult extraction of fatty acids with combination state (e.g. Lecithin, Glucolipid, Lipoprotein, etc.). Thus, NMR could be used for rapid testing of the seed oil content, which provides a reliable method for screening potential energy plants.%种子含油量是衡量能源植物开发潜力的一个重要指标.为寻找快速、准确测定种子含油量的方法,采用核磁共振法和索氏提取法测定了4种潜在能源植物的种子含油量,并对其进行了分析比较.结果表明:核磁共振法测定的种子含油量明显高于索氏提取法,其中,两种方法测得的曼陀罗种子含油量分别为28.87%和25.91%,海滨锦葵分别为17.74%和14.78%,中国沙棘分别为9.53%和6.82%,蓖麻分别为47.01%和44.31%.核磁共振法测定种子含油量具有简便、无损坏等特点,弥补了索氏提取法耗时,不能提取磷脂、糖脂、脂蛋白等结合态脂类的不足,可用于能源植物种子含油量的快速测定,为筛选潜在能源植物新种质资源提供了可靠方法.

  14. Decomposition of different plant litters in Loess Plateau of Northwest China%黄土高原不同植物凋落物的分解特性

    Institute of Scientific and Technical Information of China (English)

    李云; 周建斌; 董燕捷; 夏志敏; 陈竹君

    2012-01-01

    Taking the litters of species Hippophae rhamnoides, Medicago sativa, Populus simonii, Robinia pseudoacaci, Salix psammophila, and Stipa bungeana in the Loess Plateau of Northeast China as test objects, and by using mesh bags, this paper studied the dynamic changes of the litters mass, carbon, and nitrogen during decomposition after buried in the field in semiarid region. The litters buried were from one, two, or three of the plant species, and mixed thoroughly with equal proportion of masses. During decomposition, the mass loss rate, total carbon and nitrogen release rates, and total soluble carbon and nitrogen contents of different litters were higher at the early than at the later decomposition stage. After 412 d decomposition, the average mass loss rate of the litters was in the order of mixed litters of three plant species > mixed litters of two plant species > one plant species litter. By the end of this experiment, the average release rates of the litter total carbon and nitrogen ranked as one plant species litter > mixed litters of two plant species > mixed litters of three plant species, the litter soluble organic carbon content was mixed litters of two plant species > mixed litters of three plant species > one plant species litter, while the litter soluble total nitrogen content was mixed litters of three plant species > mixed litters of two plant species > one plant species litter. Correlation analysis showed that the utter mass loss rate had definite correlation with the litter soluble organic matter, especially soluble organic carbon. From the viewpoint of mass loss rate, the mixture of the litters of P. simonii, H. rhamnoide, and M. sativa was the optimum. It was suggested that in the process of returning farmland into forestland and grassland in the gully and valley region of Loess Plateau, it would be required to rationally increase plant species diversity to improve soil fertility.%以黄土高原区典型植物刺槐、小叶杨、沙棘、沙柳

  15. 黄土高原半干旱丘陵区不同树种纯林土壤性质极化研究%SOIL POLARIZATION UNDER PURE STANDS OF DIFFERENT TREE VARIETIES IN SEMI-ARID HILLY AREAS OF THE LOESS PLATEAU

    Institute of Scientific and Technical Information of China (English)

    刘增文; 段而军; 刘卓玛姐; 冯顺煜

    2009-01-01

    to polarization (I = P/R) were proposed for polarization analysis based on results of determination of soil properties of soil samples collected from a variety of mature stands of pure and mixed forests using a multi-point mixed sampling method at multiple sites in the semi-arid, hilly area of loess plateau. Results show very strong polarization (P>0.20) of the following soil properties: chemical properties under Pinus tabulaeformis forest; chemical and biological properties under Platycladus orientalis, Robinia pseudoacacia and Hippophae rhamnoides forests; chemical, biological properties and trace elements content under Ulmus pumila forest; biological properties under Betula platyphylla forest; and biological and physical properties under Acer ginnala forest. Among the species in this study, pure stands of P. tabulaeformis and R.pseudoacia resulted in serious depletion of soil nutrient reserves; Pure stands of P. orientalis increased soil fertility; Pure stands of U. pumila increased soil fertility but decreased trace elements content; Pure stands of B. platyphylla worsened soil biological properties; Pure stands of A. ginnala led to depletion of soil nutrient reserves and deterioration of soil biological properties; and Pure stands of H. rhamnoides did to depletion of soil nutrient reserves and degradation of soil biological and physical properties. The anti-polarization capacities of P. simonii and P. tabulaeformis were the highest and of U. pumila the lowest. Relative to anti-polarization capacity, the ratios of polarization resistance to polarization of the stands fell into the category of more than "medium" (I_a>0.40)including chemical and physical properties under P. tabulaeformis forest, all the soil properties as a whole under P. orientalis, R.pseudoacia and U. pumila forests, and biological, physical and chemical properties under B. platyphylla forest.

  16. Dynamics of Vegetation Biomass Along the Chronosequence in Hailuogou Glacier Retreated Area, Mt. Gongga%贡嘎山海螺沟冰川退缩区原生演替序列植被生物量动态

    Institute of Scientific and Technical Information of China (English)

    杨丹丹; 罗辑; 佘佳; 唐荣贵

    2015-01-01

    In order to better understand the vegetation biomass changes and distribution characteristics of different components along time, seven sampling plots (S0~S7) representing different succession stages (bare land, 17 years, 35 years, 49 years,56 years,85 years and 125 years) were chosen through investigation of the 125-year-old chronosequence in Hailuogou glacier retreated area, Mt. Gongga. Results showed that stand age and plantation types were two main factors leading to biomass change. Total living biomass of the vegetation and biomass of the tree layer both presented an exponential growth pattern along time, separately ranged from 10.195 Mg·hm-2 to 366.122 Mg·hm-2, from 9.162 Mg·hm-2 to 332.461 Mg·hm-2. Tree layer contributed most to the total living biomass, which accounted for more than 89.871 percent during all stages; in contrast, biomass of the other layers only had little influence on the total biomass and showed a tendency of shrub layer > ground cover > the herb layer. What’s more, these underwood layers were more affected by the community environment. Biomass of the coarse woody debris and annual leaves litter also accumulated during the succession chronosequence, biomass of the coarse woody debris increase to the peak at S5, where the mixed broadleaf-conifer forest was growing while the biomass of the annual leaves litter fitted well with the exponential growth model during the whole succession chronosequence. In the former 60 years of succession, biomass of the tree layer mainly came from deciduous broad-leaf species such as Salix rehderana, Hippophae rhamnoides and Populus purdomii, in the last 60 years of succession, evergreen coniferous trees, for instance, Abies fabri and Picea brachytyla contributed most to the total layer biomass (>93.070%). As for biomass allocation related to various organs, the trunk occupied the greatest proportion of the tree layer, which made up 56.388%~72.658% of the total biomass,branch and roots came secondly

  17. 沙棘油在体外对肾虚型再生障碍性贫血作用的实验研究%Experimental research on effect of sea buckthorn oil on marrow hematopoiesis in patients with aplastic anemia of kidney deficiency type in vitro GE

    Institute of Scientific and Technical Information of China (English)

    葛志红; 王栋范; 宾冬梅; 李宜真

    2008-01-01

    目的 观察沙棘油在体外对肾阳虚、肾阴虚不同证型再生障碍性贫血(AA)患者红系集落形成单位(CFU-E)、粒巨噬细胞系集落形成单位(CFU-GM)的影响.方法 20例AA患者(肾阴虚型组及肾阳虚型组各10例),正常对照组10例,取其骨髓分离单个核细胞,以琼脂半固体培养联合沙棘油的方法,培养3、7 d后分别对比各组CFU-E、CFU-GM集落形成情况.结果 不同证型AA患者的CFU-E、CFU-GM较正常对照组明显减低(P<0.01);沙棘油能明显促进不同证型AA患者CFU-E、CFU-GM的集落生成,这种促进作用随沙棘油体积分数增加而增加;在体积分数不低于20%时,沙棘油对肾阳虚患者CFU-E、CFU-GM的疗效明显好于对肾阴虚患者的疗效(P<0.05).结论 沙棘油在体外对AA患者CFU-E、CFU-GM有一定的促增殖作用,且高剂量时对肾阳虚证型患者的作用优于肾阴虚证型患者.

  18. Responses of vertical soil moisture to rainfall pulses and land uses in a typical loess hilly area, China

    OpenAIRE

    Yu, Y.; Wei, W.; Chen, L. D.; Yang, L; F. Y. Jia; H. D. Zhang

    2014-01-01

    Soil moisture plays a key role in vegetation restoration and ecosystem stability in arid and semiarid regions. The response of soil moisture to rainfall pulses is an important hydrological process, which is strongly influenced by land use during the implementation of vegetation restoration measures. In this study, vertical soil moisture variations of woodland (Pinus tabulaeformis), native grassland (Stipa bungeana), shrubland Hippophea rhamnoides), cropland (Trit...

  19. 黄土区次生植被恢复对土壤有机碳官能团的影响%Effect of revegetation on functional groups of soil organic carbon on the Loess Plateau

    Institute of Scientific and Technical Information of China (English)

    李婷; 赵世伟; 张扬; 马帅; 李晓晓

    2011-01-01

    Vegetation succession affects soil physical and chemical properties, including organic carbon functional group species, quantities, and structural changes. Changes in soil organic carbon (SOC) functional groups during the vegetation restoration process could reveal the processes governing changes in the composition and properties of humus. The study was on the Loess Plateau in the Ziwuling forest region of Shaanxi Province, where various stages of secondary forests have developed. At different phases of development, vegetation systems in this region have changed from pioneer herbage species, which initially colonized abandoned croplands, (Bothriochloa isehemum, Artenmisia giradii, Stipa bungeana, Artenmisia sacrornai, Spodiopogen sibiricus, Sophora flavecens,Themeda japonica, and Arundinella anomala) , to secondarv shrubs (Hippophae rhamnoides, Sophora viciifdia, and Ostrjopsis davidiana) , to an early forest community ( Populus davidiana, Betula platyphylla, and Platycladus orientalis with groups of trees and/or shrubs) , and finally to a Liaotungesis community forming the mature forest. The SOC content in the 0-40 cm soil layer increases significantly during the vegetation succession from abandoned cropland to mature Liaotungesis forest. The rate of increase in SOC is different during vegetation succession, and organic carbon functional groups varied among the different plant communities. Recent studies using synchrotron-based C( 1s) near-edge X-ray absorption fine structure (NEXAFS) have shown it to be an effective, non-invasive technique, which can be used to identify and fingerprint the complex structural characteristics of SOC. Consequently it can be used to investigate the impact of management on the composition and biogeochemical cycling of organic C at the molecular level in terrestrial ecosystems. This synchrotron radiation technology has rarely been used to study SOC structure in China before so we used it in this study with the objective of developing a

  20. 沙棘果汁茶饮料的研制

    Institute of Scientific and Technical Information of China (English)

    孟祥敏

    2014-01-01

    以沙棘果汁、沙棘叶浸提液为原料,二者进行调配研制开发出一种果汁茶饮料,产品富含多种营养成分及生物活性物质,能够满足人们对果汁和茶的双重喜爱,具有较好的市场前景。%Taking sea-buckthorns juice and the sea-buckthorn leaves extract as raw materials, the paper develops a kind of health drink, which is rich in a variety of nutrients and bioactive substances. The products can meet people for the double love of fruit juice and tea, and has good market prospects.

  1. Stanovení flavonoidních látek ve vybraných druzích léčivých rostlin používaných v potravinářství

    OpenAIRE

    Bačová, Romana

    2015-01-01

    In plant and food research the functional significance of herbs, spices and other plants, including their components is very popular topic. Plant material contains many components that are beneficial to human health by reducing the risk of chronic degenerative diseases. It is necessary to define the individual substances to understand and explain their biological effects. The first part of thesis describes the selected plants (elderberry, nettle, marigold, milk thistle, sea buckthorn, sage, p...

  2. “SEABUCKTHORNOLOGY” A POSSIBLE NEW INTERDISCIPLINARY SCIENCE

    OpenAIRE

    Angel PROOROCU

    2015-01-01

    The purpose of the paper was to present a critical overview on the main opinions and research results on "seabuckthornology" as a new interdisciplinary science, as mentioned on various international conferences and other events hold in different countries where the author took part and expressed his opinions as expert in the field. The current opinion of many experts is that the sea buckthorn is the result of a long hard work in the field of research, practice, landscape architecture, product...

  3. Fusarium Head Blight Control and Prevention of Mycotoxin Contamination in Wheat with Botanicals and Tannic Acid

    OpenAIRE

    Hans-Rudolf Forrer; Tomke Musa; Fabienne Schwab; Eveline Jenny; Bucheli, Thomas D.; Felix E. Wettstein; Susanne Vogelgsang

    2014-01-01

    Suspensions or solutions with 1% of Chinese galls (Galla chinensis, GC) or 1% of tannic acid (TA), inhibited germination of conidia or mycelium growth of Fusarium graminearum (FG) by 98%–100% or by 75%–80%, respectively, whereas dried bark from buckthorn (Frangula alnus, FA) showed no effect at this concentration. In climate chamber experiments where the wheat variety “Apogee” was artificially inoculated with FG and F. crookwellense (FCr) and treated with 5% suspensions of TA, GC and FA, the ...

  4. Drug: D06758 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D06758 Crude, Drug Jujube (JP16); Jujube (TN) Oleanolic acid [CPD:C17148], Betulini...c acid [CPD:C00711], Tartaric acid [CPD:C00898], Cyclic AMP [CPD:C00575], Cyclic GMP [CPD:C00942], D-Fructos...yl-2(S et R)-naringenin, Scopoletin [CPD:C01752], Rutin [CPD:C05625] Ziziphus juj...uba [TAX:326968] Same as: E00128 Therapeutic category: 5100 Rhamnaceae (buckthorn family) Jujube fruit Major... component: Zizyphus saponin Therapeutic category of drugs in Japan [BR:br08301] 5 Crude drugs and Chinese m

  5. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage.

    Science.gov (United States)

    Istrati, D; Lacatusu, I; Bordei, N; Badea, G; Oprea, O; Stefan, L M; Stan, R; Badea, N; Meghea, A

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to -45mV) and displayed average sizes of 70nm to 140nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract-bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α. PMID:27127051

  6. Woody invasions of urban trails and the changing face of urban forests in the great plains, USA

    Science.gov (United States)

    Nemec, K.T.; Allen, C.R.; Alai, A.; Clements, G.; Kessler, A.C.; Kinsell, T.; Major, A.; Stephen, B.J.

    2011-01-01

    Corridors such as roads and trails can facilitate invasions by non-native plant species. The open, disturbed habitat associated with corridors provides favorable growing conditions for many non-native plant species. Bike trails are a corridor system common to many urban areas that have not been studied for their potential role in plant invasions. We sampled five linear segments of urban forest along bike trails in Lincoln, Nebraska to assess the invasion of woody non-native species relative to corridors and to assess the composition of these urban forests. The most abundant plant species were generally native species, but five non-native species were also present: white mulberry (Morus alba), common buckthorn (Rhamnus cathartica), tree-of-heaven (Ailanthus altissima), honeysuckle (Lonicera spp.) and elm (Ulmus spp.). The distribution of two of the woody species sampled, common buckthorn and honeysuckle, significantly decreased with increasing distance from a source patch of vegetation (P = 0.031 and 0.030). These linear habitats are being invaded by non-native tree and shrub species, which may change the structure of these urban forest corridors. If non-native woody plant species become abundant in the future, they may homogenize the plant community and reduce native biodiversity in these areas. ?? 2011 American Midland Naturalist.

  7. Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage.

    Science.gov (United States)

    Istrati, D; Lacatusu, I; Bordei, N; Badea, G; Oprea, O; Stefan, L M; Stan, R; Badea, N; Meghea, A

    2016-07-01

    The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to -45mV) and displayed average sizes of 70nm to 140nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract-bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α.

  8. Surface-enhanced Raman spectroscopy studies of yellow organic dyestuffs and lake pigments in oil paint.

    Science.gov (United States)

    Mayhew, Hannah E; Fabian, David M; Svoboda, Shelley A; Wustholz, Kristin L

    2013-08-21

    Identifying natural, organic dyes and pigments is important for the conservation, preservation, and historical interpretation of works of art. Although previous SERS studies have demonstrated high sensitivity and selectivity for red lake pigments using various pretreatment conditions, corresponding investigations of yellow lake pigments and paints are relatively sparse. Here, surface-enhanced Raman scattering (SERS) spectroscopy is used to identify a variety of yellow organic dyestuffs and lake pigments in oil paint. High-quality SERS spectra of yellow dyestuffs (i.e., turmeric, old fustic, Buckthorn berries) and corresponding paints could be obtained with or without sample pretreatment using microliter quantities of HCl and methanol at room temperature. However, the SERS spectra of yellow lake pigments (i.e., Stil de Grain, Reseda lake) and their corresponding oil paints were only observed upon sample pretreatment. Ultimately, we demonstrate a reliable sample treatment protocol for SERS-based identification of turmeric, old fustic, Buckthorn berries, Stil de Grain, and Reseda lake as well as for microscopic samples of the corresponding oil paints. PMID:23722232

  9. Effect of wood ash and K-fertilization on {sup 137}Cs uptake by selected forest plants

    Energy Technology Data Exchange (ETDEWEB)

    Mandro, Yrii N. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Rosen, Klas [Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden); Vinichuk, Mykhailo M. [Zhytomyr State Technological University, P.O. Box 10005 Zhytomyr (Ukraine); Swedish University of Agricultural Sciences P.O. Box 7070 SE-750 07 Uppsala (Sweden)

    2014-07-01

    Accumulation {sup 137}Cs by different forest plants and trees after fertilization of soil with potassium and wood ash ({sup 137}Cs-contaminated and non-contaminated) in forest ecosystems of Ukraine contaminated by radionuclides after Chernobyl accident in 1986 was studied. Experiment is performed in Bazar forestry, Zhytomyr region, Ukraine, located about 70 km (51 deg. 5'35'' N, 29 deg. 18'56'' E) from Chernobyl NPP. Potassium fertilizer (KCl, wooden ash (Ash), and {sup 137}Cs-contaminated ash ({sup 137}CsAsh) in different combinations) were spread on the forest floor in April 2012 at a rate corresponding 100 kg/ha potassium. The experiment layout was as follows: 1- Control (no fertilizers were applied), 2- KCl, 3- Ash + KCl, 4- Ash + {sup 137}CsAsh, 5- Ash, 6- {sup 137}CsAsh + KCl. Samples (leaves and annual shoots) of blueberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), rowan (Sorbus aucuparia), birch (Betula), buckthorn (Frangula) and oak (Quercus robur) and also mushrooms (fruit bodies of Russula, Lactarius, Cantharellus, Collybia etc.) and berries (blueberry and cowberry) were taken monthly from each treatment. Samples were measured for {sup 137}Cs with calibrated HPGe detectors. The results of the first year studies show variation of Transfer Factors (TF) for different plants and for the same plants on different treatments. The effect of fertilization was found for blueberry on Ash-applicated (TF = 0.0085 ± 0.0025), {sup 137}CsAsh + KCl-applicated (TF = 0.0105 ± 0.0060) and Ash + KCl-applicated (TF = 0.0123 ± 0.0058) treatments compared to Control (TF = 0.0163 ± 0.0092). Also good effect for rowan on Ash + KCl-applicated treatment (TF = 0.0067 ± 0.0024) compared to Control (TF = 0.0100 ± 0.0064). Effect was less obvious for birch on Ash + KCl-applicated treatment and for cowberry on Ash + KCl-applicated treatment. There was not found an obvious effect of fertilization for buckthorn. Positive effect of

  10. ANATOMIC INVESTIGATION OF HUNGARY'S COMMON SHRUB SPECIES

    Directory of Open Access Journals (Sweden)

    Eszter ANTALFI

    2015-09-01

    Full Text Available In Hungary a huge part of wooden plants are shrubs. Flora of hungarian forests is among the richest in Europe. Many plants can be classified as shrubs or trees as well, circumstances during their development define what they will become. The diverse world of shrubs and weeds delights the eye under 20-30 meter high trees. From these there are some well known which basically everybody recognises is lilac (Syringa vulgaris, elderberry (Sambucus nigra, dog-rose (Rosa canina, single-seeded hawthorn (Crataegus monogyna and common buckthorn (Rhamnus cathartica. To get these species better known – and occasionally foreshadowing their wood industry usage in some way – it is expendient to familiarize ourselves with their microscopic structure and characteristics. Nowadays there are several imaging methods known, however for examining floral tissue the optical microscope is still the most common one to be used.

  11. Differential effects of weather and natural enemies on coexisting aphid populations.

    Science.gov (United States)

    Alyokhin, Andrei; Drummond, Francis A; Sewell, Gary; Storch, Richard H

    2011-06-01

    Study of mechanisms responsible for regulating populations of living organisms is essential for a better comprehension of the structure of biological communities and evolutionary forces in nature. Aphids (Hemiptera: Sternorrhyncha) comprise a large and economically important group of phytophagous insects distributed worldwide. Previous studies determined that density-dependent mechanisms play an important role in regulating their populations. However, only a few of those studies identified specific factors responsible for the observed regulation. Time series data used in this study originated from the untreated control plots that were a part of potato (Solanum tuberosum L.) insecticide trials in northern Maine from 1971 to 2004. The data set contained information on population densities of three potato-colonizing aphid species (buckthorn aphid, Aphis nasturtii; potato aphid, Macrosiphum euphorbiae; and green peach aphid, Myzus persicae) and their natural enemies. We used path analysis to explore effects of weather and natural enemies on the intrinsic growth rates of aphid populations. Weather factors considered in our analyses contributed to the regulation of aphid populations, either directly or through natural enemies. However, direct weather effects were in most cases detectable only at P ≤ 0.10. Potato aphids were negatively affected by both fungal disease and predators, although buckthorn aphids were negatively affected by predators only. Parasitoids did not have a noticeable effect on the growth of any of the three aphid species. Growth of green peach aphid populations was negatively influenced by interspecific interactions with the other two aphid species. Differential population regulation mechanisms detected in the current study might at least partially explain coexistence of three ecologically similar aphid species sharing the same host plant.

  12. Study on the Processing of Seabuckthorn Wine with Low Alcohol%低度沙棘酒配制工艺研究

    Institute of Scientific and Technical Information of China (English)

    张军

    2014-01-01

    this experiment mainly studied the optimal modulation technology of seabuckthorn wine with low alcohol. The main factors of base liquor consumption, seabuckthorn juice content, spices amount by single factor experiments,but the effect of following factors to liquor sensory quality has been anal-ysised by L18(37) orthogonal test,including the alcohol degree,base liquor,sugar,the amount of sea-buckthorn juice, flavor fragrance flavor, sorghum essence, ethyl maltol and etc. The best formula is as follows: 38% of alcohol, 35% of wine base, 16% of sea buckthorn juice, 4% of sugar, 0.015% of Qing scent flavor,0.015% of sorghum essence,0.013% of ethyl maltol,the quality index reached the enterprise standard for quality requirements.%本试验主要研究低度沙棘酒配制的最佳调制工艺。对主要因素基酒用量、沙棘清汁用量、香料用量采用单因素试验,采用L18(37)正交试验全面考查酒精度、基酒用量、糖用量、沙棘清汁、高粱香精、清香型香精、乙基麦芽酚等因素对酒质感官质量影响,获得最佳配方是:酒精度38%、酒基35%、沙棘清汁16%、冰糖4%、清香型香精0.015%、高粱香精0.015%、乙基麦芽酚0.013%,质量指标达到企业标准优级要求。

  13. Palynological investigation of a Holocene profile section from the Palaeo-Gaxun-Nur-Basin

    Institute of Scientific and Technical Information of China (English)

    D. Demske; S. Mischke

    2003-01-01

    Palynological investigations on lacustrine sediment samples revealed ageneral dominance of open Artemisia-Chenopodiaceae vegetation in the Palaeo-Gaxun- Nur-Basin (Inner Mongolia) between 5250 and 3500 cal. aBP. Riverside woods (Hippophae, Populus) developed between 3500-3250 cal. aBP and reflect moister growing conditions. During the following moist-dry transition pronounced cooling occurred around 3000 cal. aBP, in upper elevations favouring the spread of montanewoods (Picea, Betula). Aridity strongly increased from 2900 to 2700 cal. aBP. The recorded vegetational and environmental changes after 3500 cal. aBP can be correlated with glacier advances in the mountains and with a humid phase in the Tengger Shamo followed by desertification.

  14. Primeros resultados de la caracterización botánica de mieles producidas por Tetragonisca angustula (Apidae, Meliponinae en Los Naranjos, Salta, Argentina First results of botanical characterization of honeys produced by Tetragonisca angustula (Apidae, Meliponinae in Los Naranjos, Salta, Argentina

    Directory of Open Access Journals (Sweden)

    F. F Flores

    2010-06-01

    Full Text Available Se realizó el análisis melisopalinológico de diez muestras de miel de Tetragonisca angustula Latreille criadas en la localidad Los Naranjos (S 23° 07' 19,2"; O 64° 40' 32,6", Salta, Argentina. Se reconoció un total de 49 tipos polínicos. En las mieles monoflorales, los tipos dominantes correspondieron a: Anadenanthera colubrina, Baccharis, Citrus sp., Myrtaceae nativas y un tipo indeterminado (tipo 6. Las restantes muestras fueron multiflorales. El 62 % de los tipos polínicos corresponde a representantes de la Selva Montana de Yungas; tipos como Anadenanthera colubrina, Bocconia integrifolia, Parapiptadenia excelsa, Phyllostylon rhamnoides, Sapium haematospermum, tipo Myrsine y un conjunto de Myrtaceae nativas que constituyen la primera cita como plantas nectaríferas para T. angustula. También se identificó polen proveniente de plantas típicas de ambientes antropizados como Carica papaya, Citrus sp., Eucalyptus sp., Leonurus sibiricus, entre otros. Estos resultados constituyen una primera aproximación al conocimiento de la flora utilizada por T. angustula y al origen geográfico de sus mieles, estableciendo el primer registro para Selva Montana de Yungas.The melissopalynological analysis was performed on ten honeys samples of Tetragonisca angustula Latreille breeding in the locality Los Naranjos (S 23° 07' 19,2"; W 64° 40' 32,6", Salta, Argentina. We found a whole of 49 pollen types. In the honey monofloral, the dominant types corresponded to: Anadenanthera colubrina, Baccharis, Citrus sp., native Myrtaceae and a type undetermined (type 6. The remaining samples were multifloral. 62% of pollen types corresponding to representatives of the Yungas mountain forest; types like Anadenanthera colubrina, Bocconia integrifolia, Parapiptadenia excelsa, Phyllostylon rhamnoides, Sapium haematospermum, type Myrsine and a set of native Myrtaceae, which constitute the first appointment as nectar sources for T. angustula. Also identified pollen

  15. Responses of insect pests, pathogens, and invasive plant species to climate change in the forests of northeastern North America

    Energy Technology Data Exchange (ETDEWEB)

    Dukes, J.S.; Theoharides, K.A. [Massachusetts Univ., Boston, MA (United States). Dept. of Biology; Pontius, J. [United States Dept. of Agriculture, Durham, NH (United States). Northern Research Station; Orwig, D.; Stinson, K. [Harvard Univ., Petersham, MA (United States); Garnas, J.R.; Stange, E.E.; Ayres, M. [Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences; Rodgers, V.L. [Boston Univ., Boston, MA (United States). Dept. of Biology; Brazee, N. [Massachusetts Univ., Amherst, MA (United States). Dept. of Plant, Soil, and Insect Sciences; Cooke, B. [Canadian Forest Service, Northern Forestry Centre, Edmonton, AB (Canada); Harrington, R. [Massachusetts Univ., Amherst, MA (United States). Natural Resource Conservation; Ehrenfield, J. [Rutgers Univ., New Brunswick, NJ (United States). Dept. of Ecology, Evolution, and Natural Resources; Gurevitch, J. [New York State Univ., Stony Brook, NY (United States). Dept. of Ecology and Evolution; Lerdau, M. [Virginia Univ., Charlottesville, VA (United States). Dept. of Environmental Sciences and Biology; Wick, R. [Massachusetts Univ., Amherst, MA (United States). Dept. of Microbiology

    2009-02-15

    In addition to increased winter precipitation, it is expected that the climate in the northeastern United States and Canada will increase by approximately 3 to 5 degrees C. While the changes in climate will impact the trees directly, the trees will be further impacted by changes in insect pests, pathogens, and invasive plants. This study reviewed the basic ecological principles used to predict the responses of nuisance species to climate change as well as their likely impacts on northeastern forests. The study investigated the potential responses of hemlock woolly adelgid; forest tent caterpillars; armillaria root rot; beech bark disease; glossy buckthorn; and oriental bittersweet. Results of the study indicated that several of the studied species are likely to have a more widespread impact on forest composition and structure in the future. However adequate data on species are not available in order to fully understand the influence of complex variables for the studied species. It was concluded that future policies should allow for uncertainty in prediction models considering a range of possible future scenarios. 181 refs., 4 tabs., 3 figs.

  16. Polyphenols-rich natural products for treatment of diabetes.

    Science.gov (United States)

    Dragan, S; Andrica, F; Serban, Maria-Corina; Timar, R

    2015-01-01

    Currently, experimental and clinical evidences showed that polyphenols-rich natural products, like nutraceuticals and food supplements, may offer unique treatment modalities in type 2 diabetes mellitus (DM), due to their biological properties. Natural products modulate the carbohydrate metabolism by various mechanisms, such as restoring beta-cells integrity and physiology, enhancing insulin releasing activity, and the glucose using. Sea buckthorn berries, red grapes, bilberries, chokeberries and popular drinks like cocoa, coffee and green tea are all rich in polyphenols and may decrease the insulin response, offer in g a natural alternative of treatment in diabetes. Therefore, researches are now focused on potential efficacies of different types of polyphenols, including flavonoids, phenolic acids, lignans, anthocyans and stilbenes. Animal and human studies showed that polyphenols modulate carbohydrate and lipid metabolism, decrease glycemia and insulin resistance, increase lipid metabolism and optimize oxidative stress and inflammatory processes. It is important to understand the proper dose and duration of supplementation with polyphenols-rich extracts in order to guide effective therapeutic interventions in diabetic patients.

  17. Acceptance of Nordic snack bars in children aged 8–11 years

    Directory of Open Access Journals (Sweden)

    Anna Holmer

    2012-04-01

    Full Text Available Background: A health promoting diet is suggested to be tailored to regional circumstances to preserve the cultural diversity in eating habits, as well as contribute to more environmentally friendly eating. It may influence consumer acceptance, however, if the components of the diet differs considerably from their habitual food. Objective: This study aimed to investigate whether snack bars composed of Nordic ingredients were accepted by 8–11 year-old Danish (n=134 and Swedish (n=109 children.Design: A seven-point hedonic scale was used to measure the children's acceptance of five snack bars that varied in their composition of whole grains, berries and nuts. A preference rank ordering of the five bars was also performed. Results: The results showed that samples that were rated highest in liking and were most preferred in both countries were a kamut/pumpkin bar and an oat/cranberry bar. The sample with the lowest rating that was also least preferred was a pumpernickel/sea buckthorn bar. Flavour was the most important determinant of overall liking followed by texture, odour and appearance. Conclusions: Children's acceptances and preferences were highly influenced by the sensory characteristics of the bars, mainly flavour. In agreement with earlier studies, the novel food ingredients seemed to influence children's preferences. The Nordic snack bars may have a potential to be a snack option for Danish and Swedish school children, but repeated exposures to the products are recommended to increase children's acceptance.

  18. [Benzo(a)pyrene contamination of vegetable oils].

    Science.gov (United States)

    Jedra, Małgorzata; Starski, Andrzej; Gawarska, Halina; Sawilska-Rautenstrauch, Dorota

    2008-01-01

    Benzo(a)pyrene (B(a)P) analysis was carried out with glass chromatographic column with alumina followed by reverse phase high-performance liquid chromatography (HPLC) and spectrofluorometric detection. B(a)P level in 40 vegetable oils were as follow: from 0.11 to 0.38 microg/kg in olive; from 0.92 to 3.74 microg/kg in rape seed oils; from 0.11 to 2.25 microg/kg in sunflower oils and from 0.33 to 1.26 microg/kg in soya oils. In another investigated oils: arachide (peanut) corn, safflower, linen, hempen, sesame, pumpkin seeds, grape seeds---values from 0.10 to 1.44 microg/kg and 3.83 microg/kg in sea buckthorn oil were detected. B(a)P concentration in 4 from 40 investigated oils exceed the 2 ppb limit proposed by the European Commission. Heating of sample of oils: olive, rape, soya, linen, corn, sesame, peanut, in temp. 240 degrees C for 30 min. has not influence on decreased of B(a)P level. PMID:18807910

  19. A review of characterization of tocotrienols from plant oils and foods.

    Science.gov (United States)

    Ahsan, Haseeb; Ahad, Amjid; Siddiqui, Waseem A

    2015-04-01

    Tocotrienols, members of the vitamin E family, are natural compounds found in a number of vegetable oils, wheat germ, barley and certain types of nuts and grains. Vegetable oils provide the best sources of these vitamin E forms, particularly palm oil and rice bran oil contain higher amounts of tocotrienols. Other sources of tocotrienols include grape fruit seed oil, oats, hazelnuts, maize, olive oil, buckthorn berry, rye, flax seed oil, poppy seed oil and sunflower oil. Tocotrienols are of four types, viz. alpha (α), beta (β), gamma (γ) and delta (δ). Unlike tocopherols, tocotrienols are unsaturated and possess an isoprenoid side chain. A number of researchers have developed methods for the extraction, analysis, identification and quantification of different types of vitamin E compounds. This article constitutes an in-depth review of the chemistry and extraction of the unsaturated vitamin E derivatives, tocotrienols, from various sources using different methods. This review article lists the different techniques that are used in the characterization and purification of tocotrienols such as soxhlet and solid-liquid extractions, saponification method, chromatography (thin layer, column chromatography, gas chromatography, supercritical fluid, high performance), capillary electrochromatography and mass spectrometry. Some of the methods described were able to identify one form or type while others could analyse all the analogues of tocotrienol molecules. Hence, this article will be helpful in understanding the various methods used in the characterization of this lesser known vitamin E variant.

  20. Antioxidant capacities and total polyphenol contents of hydro-ethanolic extract of phytococktail from trans-Himalaya.

    Science.gov (United States)

    Dhar, P; Tayade, A B; Bajpai, P K; Sharma, V K; Das, S K; Chaurasia, O P; Srivastava, R B; Singh, S B

    2012-02-01

    The aim of this study was to evaluate the in vitro antioxidant potential of hydro-ethanolic extract of a novel phytococktail comprising of sea buckthorn, apricot, and Rhodiola (SAR) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) activity of the extract increased in a dose-dependent manner (upto 0.1 mg/mL), and was found to be about 38% of that of ascorbic acid at 0.1 mg/mL. The hydro-ethanolic extract of SAR also scavenged the ABTS(.+) radical generated by ABTS/potassium persulfate (PPS) system and was found to be about 62% of that of ascorbic acid at 0.1 mg/ mL. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total phenolic content was found to be 1.28016 × 10(-3) mol gallic acid equivalent (GAE)/g extract. Total flavonoid and flavonol contents were estimated to be 2.5970 × 10(-4) mol and 4.87 × 10(-4) mol quercetin equivalent/g extract, respectively. The hydro-ethanolic extract of this phytococktail indicated presence of essential phytoconstituents of polyphenols, flavonoids, flavonols, and ascorbic acid, which contributed significantly to its antioxidant capacity. The combination of the 3 plants may well support their use in traditional medicine to combat oxidative stress and high-altitude sickness. PMID:22225422

  1. Study on Seabuckthorn Compound Juice Recipe%沙棘复合果蔬汁饮料配方研究

    Institute of Scientific and Technical Information of China (English)

    宋翔; 李悦; 魏文静; 康明艳

    2015-01-01

    以沙棘、西红柿、胡萝卜3种果蔬汁为原料生产复合果蔬饮料,通过正交试验筛选出该复合保健果蔬汁的最佳配比应为:沙棘汁20%、西红柿汁10%、胡萝卜汁15%、蔗糖10%。酸味剂选择柠檬酸,用量为0.15%;护色剂选用茶多酚,用量为0.12%;稳定剂选用CMC-Na和海藻酸钠组成复合稳定剂,海藻酸钠用量0.15%、CMC-Na用量1.2%。%Seabuckthorn, tomatoes, carrots 3 kinds of juice as raw material to produce composite fruit and vegetable juice, orthogonal experiment was used to select optimum ratio of the composite health care fruit and vegetable juice should be:sea buckthorn juice 20%, tomato juice 10%, sugar 10%, Fresh Carrot Juice 15%. Acid and citric acid, dosage of 0.15%;color protecting agent selection of tea polyphenols, dosage was 0.1%;the stabilizer CMC-Na and sodium alginate composite stabilizer , sodium alginate was 0.10%, the dosage of CMC-Na 15%.

  2. Effect of the combination of natural antioxidants and packaging methods on quality of pork patties during storage.

    Science.gov (United States)

    Kumar, Vikas; Chatli, Manish K; Wagh, Rajesh V; Mehta, Nitin; Kumar, Pavan

    2015-10-01

    The effect of combination of phyto-extracts (sea buckthorn extract (SBTE), grape seed extract (GSE)) on different physico-chemical, oxidative stability, instrumental colour and texture, sensory and microbiological properties of pork patties were investigated under aerobic and MAP (50 % CO2: 50 % N2) packaging conditions. Treatments viz. T-1 (aerobic packaged control), T-2 (aerobic packaged containing 0.3 % SBTE+ 0.1 % GSE), T-3 (MAP packaged control) and T-4 (MAP packaged containing 0.3 % SBTE+ 0.1 % GSE) at 4 ± 1 °C for 35 days and samples were drawn at 7 days interval. The pH decreased initially up to 21 days and thereafter increased on further storage whereas water activity followed a decreasing trend throughout the storage period, irrespective of the treatment and packaging conditions. Results of oxidative stability parameters revealed that peroxide value, TBARS and FFA followed an increasing trend in both the packaging groups during storage however, the rate of increase was significantly lower (P Standard plate count, psychrophiles and Coliforms were significantly (P count was better maintained in MAP than aerobic condition. Results concluded that the combined use of antioxidants and MAP packaging would be a useful method to control the oxidative and microbial quality changes of pork patties and it can be successfully stored for 35 days.

  3. Proposition de classement des sources végétales d’acides gras en fonction de leur profil nutritionnel

    Directory of Open Access Journals (Sweden)

    Dubois Virginie

    2008-01-01

    Full Text Available The role of isolated fatty acids on human health has long been studied by the nutritionists. The consequence of this research for the food industry was a need for a better control of the lipidic profile of manufactured food products. The purpose of the present paper was to give the nutritional profile of 53 vegetable oils in relation with their fatty acid composition and their related nutritional potential. In a first section, the role of isolated fatty acids on blood lipids (LDL-Cholesterol, HDL-Cholesterol and triacylglycerols has been reviewed. In a second section, the vegetable oils are gathered in relation with their similar fatty acids composition, with the aim of classifying their properties in accordance with their role on blood lipids. From a nutritional point of view, besides the major α-linolenic acid source that is rapeseed oil, the classification makes some interesting sources to stand out. They contain either mainly α-linolenic acid (camelin, linseed and perilla or both essential fatty acids as α-linolenic and linoleic acids (purslane, chia, salicorna and sea buckthorn seeds. Moreover, this collection is an interesting tool for new designed food products with a better lipid composition.

  4. Improving soil bioengineering techniques to control erosion and sedimentation within the context of torrential Mediterranean climate: a French-Canadian experience

    Science.gov (United States)

    Rey, Freddy; Louis, Séverine; Burylo, Mélanie; Raymond, Pierre

    2013-04-01

    On marly eroded terrains of the French Southern Alps, many researches are undertaken in order to better understand the role of vegetation and bioengineering works on erosion and sedimentation control. To this view, the eroded marly gullies of the French Southern Alps are an experimental design where an original French strategy of rehabilitation, developed by scientists from Irstea (ex-Cemagref), has been tested since 2002. It is comprised of the construction of bioengineering works, namely of "brush layers and brush mats of cuttings on deadwood microdams", and implements the use of willow cuttings (Salix purpurea and S. incana). The main objective of these works is to sustainably trap and retain marly sediment, by checking their performance (growth and survival of the cuttings, sediment trapping) in a mountainous and Mediterranean climate. In Canada, several private companies have developed their own knowledge and expertise in the conception and building of bioengineering works for erosion control, especially in the context of hilly and mountainous landscapes and climates. Therefore, it was decided to use the competence and expertise of Terra Erosion Control Ltd., a Canadian company, in the French torrential Mediterranean climate. Ten modalities were tested, the aims being to develop and/or to modify existing designs of current techniques, to experiment with other live cuttings (Populus nigra) and rooted species (Alnus spp. and Hippophae spp.), to evaluate and compare the potential use of different organic soil amendments in order to increase beneficial soil microorganisms and finally, to evaluate the potential use of specialized tools and equipment in order to increase the efficiency of the installation for vegetation establishment and sediment trapping, while decreasing the implementation costs. The experimental design was installed in March 2011 and the early observations in Spring 2012 showed that: 1/ most of the cuttings and the plants resisted to burial and

  5. Antioxidant and Antiproliferative Activities of Five Wild Berries%5种野生浆果的抗氧化和抗细胞增殖活性

    Institute of Scientific and Technical Information of China (English)

    樊梓鸾; 王振宇; 程翠林; 赵海田; 张华

    2010-01-01

    选取东北大兴安岭地区的特色浆果化楸(Sorbus pohuashanensis H.)、蓝莓(Vaccinium uliginosum L.)、蓝靛果(Lonicera caerulea L.)、沙棘(Hipophae rhamnoides L.)、草莓(Fragaria orientalis Los.)的成熟果实,采用80%丙酮提取,分别测定多酚、黄酮、花色苷含量,并研究其抗氧化活性和抗细胞增殖活性.结果表明:花楸的多酚和黄酮含量最高;蓝靛果的花色苷含量最高,为(367.5±8.7)g/100g;5种浆果都具有较好的体外抗氧化活性,花楸显示了最高的抑制痛细胞增殖效应.

  6. Responses of vertical soil moisture to rainfall pulses and land uses in a typical loess hilly area, China

    Science.gov (United States)

    Yu, Y.; Wei, W.; Chen, L. D.; Jia, F. Y.; Yang, L.; Zhang, H. D.; Feng, T. J.

    2015-05-01

    Soil moisture plays a key role in vegetation restoration and ecosystem stability in arid and semiarid regions. The response of soil moisture to rainfall pulses is an important hydrological process, which is strongly influenced by land use during the implementation of vegetation restoration. In this study, vertical soil moisture variations of woodland (Pinus tabulaeformis), native grassland (Stipa bungeana), shrubland (Hippophea rhamnoides), cropland (Triticum aestivum) and artificial grassland (Onobrychis viciaefolia) in five soil profiles were monitored in a typical loess hilly area during the 2010 growing season. The results demonstrated that rainfall pulses directly affected soil moisture variation. A multi-peak pattern of soil moisture appeared during the growing season, notably in the surface soil layer. Meanwhile, the response of each vegetation type to rainfall was inconsistent, and a time-lag effect before reaching the peak value was detected, following each heavy rainfall event. The response duration of soil moisture, however, varied markedly with the size of rainfall events. Furthermore, higher soil water content was detected in grassland and shrubland. Woodland was characterized by relatively lower soil moisture values throughout the investigation period. Our research suggests that vegetation restoration efforts should give priority to grassland and shrubland at the research site. We suggest that more studies should be focused on the characteristics of community structure and spatial vegetation distribution on soil moisture dynamics, particularly within the grass and shrub ecosystems.

  7. Phytochemicals with radioprotection and radio-sensitizing potential

    International Nuclear Information System (INIS)

    Ionizing radiation induces DNA damage and are harmful to mankind. They act through free radical generation, which target the double bonds of all cellular macromolecules. The radiation damage may be classified as probabilistic or deterministic, depending on the dose of radiation exposure. Since radiation affects multiple organs so those drugs which protect many organs, would be more beneficial. In this process herbal extracts, which are cocktail of several phyto-chemicals, would be more promising. Initially sulphur containing bio-molecules were identified as radio-protector, but now many secondary metabolites from plant kingdom, have been reported to be radio-protective. They have different mechanism of action, but most of them either prevent the FR induced DNA damage or accelerate the DNA repair process. Aminofostine, WR-2721, 159243, 2926 are some of the examples. However they have limited use because of associated cytotoxicity. Eicosanoids, topoisornerase inhibitors (e.g. camptothecin, topotecan), and the hypoxia-activated anthraquinone AQ4N have shown radioprotecting potential. Several plant products, derived from Tulsi, Vinca alkaloids, taxans, turmeric, Rubia cordifolia, Semecarpus anacardium and several plants rich in polyphenols and flavones have shown hemotherapeutic potential. Similarly, Hippophae, rhodiola imbricata, Podophyllum hexandrum, Ocimum sancturn, Plumbago zeylanica etc have shown radioprotection. Rubia cordifolia has shown both chemotherapeutic and radioprotective property in rats and A-431 cells. Similarly Semecarpus anacardium extract has shown cell cycle arrest in DU-145 cells. (author)

  8. Isorhamnetin ameliorates LPS-induced inflammatory response through downregulation of NF-κB signaling.

    Science.gov (United States)

    Li, Yang; Chi, Gefu; Shen, Bingyu; Tian, Ye; Feng, Haihua

    2016-08-01

    Isorhamnetin, a flavonoid mainly found in Hippophae fhamnoides L. fruit, has been known for its antioxidant activity and its ability to regulate immune response. In this study, we investigated whether isorhamnetin exerts potent antiinflammatory effects in RAW264.7 cell and mouse model stimulated by LPS. The cytokine (TNF-α, IL-1β, and IL-6) levels were determined. In the mouse model of acute lung injury, the phosphorylation of NF-κB proteins was analyzed and inhibitor of NF-κB signaling (PDTC) was used on mice. Our results showed that isorhamnetin markedly decreased TNF-α, IL-1β, and IL-6 concentrations and suppressed the activation of NF-κB signaling. Meanwhile, isorhamnetin reduced the amount of inflammatory cells, the lung wet-to-dry weight ratio, protein leakage, and myeloperoxidase activity. Interference with specific inhibitor revealed that isorhamnetin-mediated suppression of cytokines and protein was via NF-κB signaling. So, it suggests that isorhamnetin might be a potential therapeutic agent for preventing inflammatory diseases. PMID:27138362

  9. Ethnomedicine in Himalaya: a case study from Dolpa, Humla, Jumla and Mustang districts of Nepal

    Directory of Open Access Journals (Sweden)

    Kshhetri Hari B

    2006-06-01

    Full Text Available Abstract Traditional plant use in Nepal has been documented for millennia. The importance of plants as medicine has not diminished in any way in recent times, and traditional medicines are still the most important health care source for the vast majority of the population. This paper examines the ethnobotany and traditional use of plants extracted from the vulnerable alpine zone in the Dolpa, Humla, Jumla and Mustang districts of Nepal. The results of this ethnobotanical study indicate that a very large number of plant species is used as traditional medicines. There were 107, 59, 44 and 166 species of ethnomedicinal importance in surveyed areas of Dolpa, Humla, Jumla and Mustang district respectively. Of these, 84 common species, used at least in two districts, were selected to enumerate their ethnomedicinal properties. The 84 species belonged to 75 genera and 39 families. The commonest species in this pharmacopoeia were: Allium wallichii, Cordyceps sinensis, Dactylorhiza hatagirea, and Rheum australe. A total of 21 species were most common in three districts and 59 in two districts. The genera Aconitum, Allium, Arisaema, Berberis, Corydalis, Gentiana, Hippophae, Juniperus and Rhododendron each possessed two species with ethnomedicinal use. Labiatae was the most medicinally important family with five species used, followed by Araceae, Compositae, Liliaceae, Polygonaceae, Ranunculaceae, Scrophulariaceae and Umbelliferae, each contributing four species.

  10. [Difference of anti-fracture mechanical characteristics between lateral-root branches and adjacent upper straight roots of four plant species in vigorous growth period].

    Science.gov (United States)

    Liu, Peng-fei; Liu, Jing; Zhu, Hong-hui; Zhang, Xin; Zhang, Ge; Li, You-fang; Su, Yu; Wang, Chen-jia

    2016-01-01

    Taking four plant species, Caragana korshinskii, Salix psammophila, Hippophae rhamnides and Artemisia sphaerocephala, which were 3-4 years old and in vigorous growth period, as test materials, the anti-fracture forces of lateral-root branches and adjacent upper straight roots were measured with the self-made fixture and the instrument of TY 8000. The lateral-root branches were vital and the diameters were 1-4 mm. The results showed that the anti-fracture force and anti-fracture strength of lateral-root branches were lesser than those of the adjacent upper straight roots even though the average diameter of lateral-root branches was greater. The ratios of anti-fracture strength of lateral-root branches to the adjacent upper straight roots were 71.5% for C. korshinskii, 62.9% for S. psammophila, 45.4% for H. rhamnides and 35.4% for A. sphaerocephala. For the four plants, the anti-fracture force positively correlated with the diameter in a power function, while the anti-fracture strength negatively correlated with diameter in a power function. The anti-fracture strengths of lateral-root branches and adjacent upper straight roots for the four species followed the sequence of C. korshinskii (33.66 and 47.06 MPa) > S. psammophila (17.31 and 27.54 MPa) > H. rhamnides (3.97 and 8.75 MPa) > A. sphaerphala (2.18 and 6.15 MPa).

  11. Dynamics of Soil Water Content under Different Forestland in the Loess Hilly Region%黄土丘陵区不同林地土壤水分动态变化

    Institute of Scientific and Technical Information of China (English)

    王晶; 朱清科; 刘中奇; 邝高明; 赵荟

    2011-01-01

    研究黄土丘陵区不同林地的土壤水分变化,对于改善黄土高原生态环境建设有着重要的意义.采用定点监测的方法,对陕西省吴起县不同林地土壤水分动态变化进行了研究.结果表明:不同林地在生长季中土壤蓄水量具有显著差异,其顺序为:沙棘林>河北杨林>油松林>山杏林;林地的土壤蓄水量变化具有明显的季节性,根据生长季内土壤蓄水量的变化,可以将土壤水分变化分为3个时期:土壤水分恢复期、土壤水分消耗期、土壤水分补充期;各林地0-200 cm土层土壤水分变异系数从小到大为:山杏林>河北杨林>沙棘林>油松林;在雨水人渗、蒸发、蒸散的共同作用下山杏林地在0-60 cm土层含水量的变化明显大于其它林地.研究结果揭示了不同林种对土壤水分消耗和补给的影响,为当地造林结构配置和植被恢复与重建工作提供一定参考依据.%Soil water content is crucial to vegetation rehabilitation on the hilly loess plateau.Using fixed-point dynamic monitoring methods, dynamics of soil water content under different forest were studied in forest or grass lands converted from farmlands in loess hilly region.Results showed that different forestland has a significant differences on soil water storage capacity in the growing season, in terms of soil water, forest species may be ranked in the descendant order of sea buckthorn, hebei poplar, Chinese pine, prunus armeniaca; forestland soil water storage has obvious seasonal change, according to growing season changes in soil water storage, soil moisture changes can be divided into three period: recovery period of soil moisture, consumption period of soil water, replenishment period of soil moisture; in the 0-200 cm soil layer, the variation coefficient of soil water in forestland may be ranked in the descendant order of prunus armeniaca, hebei poplar, sea buckthorn, Chinese pine.In the action of rainwater infiltration, evaporation

  12. Tecnology of development phytogenetic protector's andcreation of raw-material base

    International Nuclear Information System (INIS)

    Full tex: Recently in order to protect organisms from the of radiation effect radio protectors have been applied principally concerning to sulfur compounds, aminothioles and indolilalkins. Although the achievements obtained in the direction of synthetic medicines discovery, they can not completely replace the medicinal preparation of natural origin. That is why the study of natural biological active substances with radio protector property are of special importance.One of the goals of the project is the detection of plant species rich with anti mutagen and radio protector, the study of their cultivation technology, and the produce of natural raw material with high quality in planting condition. Flora of Azerbaijan is considered one of the richest countries for its species' richness. There are plant varieties for producing radio protector substances (essence-oil, lipid, lipoid, phenol compounds, pigment substances, proteins, vitamins and various amino acids). We have cultivated new varieties and forms of sea buckthorn, black caraway, basil, tarragons which are richer with their radio protector substances among these plants. Our aim in producing new varieties and forms is to get new varieties which rich with protector property substances, by conducting selection in condition of the cultivation without touching the balance of natural biodiversity and to protect the species with few natural resources.In the article it is intended to prepare aerosol, consisted of essence oils and lipid mixtures, for respiratory system treatment in extreme conditions having radiation threat. The plants with essence oil are potential radio protectors.A technology will be worked out for the produce of cream, protecting surrounding tissues during radiation therapy; for producing radio protector propertied oils from these plant, fruits and seeds in order to treat radioactive burns of digestive system and skin; balsam producing for prophylactic purposes; hygienic remedies such as shampoo

  13. Capillary electrophoresis as a novel technique for screening natural flavonoids as kinase inhibitors.

    Science.gov (United States)

    Nehmé, Reine; Nehmé, Hala; Roux, Grégory; Destandau, Emilie; Claude, Bérengère; Morin, Philippe

    2013-11-29

    Capillary electrophoresis (CE) was used for the first time to evaluate the inhibition activity of aglycone flavonoids (such as quercetin and isorhamnetin) and some of their glycosylated derivatives toward human kinases. The cyclin-dependant kinase 5 (CDK5/p25) and the glycogen synthase kinase 3β (GSK3β) were chosen since they are very promising biological targets for developing treatments against neurodegenerative diseases and cancer. In a previous work, we developed an in-capillary kinase CE assay where the capillary was used as an enzymatic nanoreactor in which the kinase, its substrate, adenosine 5'-triphosphate (ATP) and its potential inhibitor were mixed by using transverse diffusion of laminar flow profiles (TDLFP). The product adenosine 5'-diphosphate (ADP) was then detected at 254nm and quantified. In this work, this assay was improved to reduce, for the first time, the dilution effect commonly observed with the TDLFP approach. Under the new conditions established herein, IC50 values for quercetin, kaempferol and flavopiridol were successfully obtained and were in the same order of magnitude of those reported in the literature using the conventional assay using radioactive (33)P-ATP. It was shown that aglycone flavonoids have an inhibition activity more important than their glycosylated derivatives. CE was also proved to be very efficient for evaluating inhibition activity of complex samples such as crude extracts of sea buckthorn (SBT) berries obtained by solvent-free microwave extraction (SFME). This novel approach to combine SFME technique to a CE-based enzymatic assay is very interesting for evaluating the biological activity of natural material in a fast, simple, economic (no use of neither fluorescent nor radiometric labels) and green (no organic solvents) manner. PMID:24148298

  14. Indigenous use and bio-efficacy of medicinal plants in the Rasuwa District, Central Nepal

    Directory of Open Access Journals (Sweden)

    Boon Emmanuel K

    2010-01-01

    Full Text Available Abstract Background By revealing historical and present plant use, ethnobotany contributes to drug discovery and socioeconomic development. Nepal is a natural storehouse of medicinal plants. Although several ethnobotanical studies were conducted in the country, many areas remain unexplored. Furthermore, few studies have compared indigenous plant use with reported phytochemical and pharmacological properties. Methods Ethnopharmacological data was collected in the Rasuwa district of Central Nepal by conducting interviews and focus group discussions with local people. The informant consensus factor (FIC was calculated in order to estimate use variability of medicinal plants. Bio-efficacy was assessed by comparing indigenous plant use with phytochemical and pharmacological properties determined from a review of the available literature. Criteria were used to identify high priority medicinal plant species. Results A total of 60 medicinal formulations from 56 plant species were documented. Medicinal plants were used to treat various diseases and disorders, with the highest number of species being used for gastro-intestinal problems, followed by fever and headache. Herbs were the primary source of medicinal plants (57% of the species, followed by trees (23%. The average FIC value for all ailment categories was 0.82, indicating a high level of informant agreement compared to similar studies conducted elsewhere. High FIC values were obtained for ophthalmological problems, tooth ache, kidney problems, and menstrual disorders, indicating that the species traditionally used to treat these ailments are worth searching for bioactive compounds: Astilbe rivularis, Berberis asiatica, Hippophae salicifolia, Juniperus recurva, and Swertia multicaulis. A 90% correspondence was found between local plant use and reported plant chemical composition and pharmacological properties for the 30 species for which information was available. Sixteen medicinal plants were

  15. A revised molecular phylogeny of the globally distributed hawkmoth genus Hyles (Lepidoptera: Sphingidae), based on mitochondrial and nuclear DNA sequences.

    Science.gov (United States)

    Hundsdoerfer, Anna K; Rubinoff, Daniel; Attié, Marc; Wink, Michael; Kitching, Ian J

    2009-09-01

    The hawkmoth genus Hyles comprises some 29 species with a global distribution. In this study, we augment the previous taxon sampling with more species and add sequences from a nuclear gene to produce a refined phylogenetic hypothesis. A total evidence reconstruction based on Bayesian analysis of the combined mitochondrial (COI, t-RNA-Leu, COII; 2284 bp) and nuclear (EF1alpha; 773 bp) sequences is discussed and compared with the results from separate analyses of the two genes. The total evidence phylogeny corroborates many of the phylogenetic relationships previously postulated within the genus. In addition, the hitherto unsampled enigmatic species Hyles biguttata from Madagascar appears as sister group to Hyles livornicoides from Australia, although support for the relationship is relatively weak. The high level of differentiation of Hyles perkinsi from H. calida (both Hawaii), and the status of these two as sister species, is corroborated by both sources of sequence data. However, their phylogenetic position when mt DNA sequences alone are considered differs markedly from that under total evidence. The previously postulated relationships within the Hyles euphorbiae complex (HEC) s.s. are largely corroborated, but H. dahlii is now more closely related and the HEC s.l. is redefined to include H. zygophylli and H. stroehlei (two species that had not been studied previously using molecular data) and to exclude H. siehei and H. hippophaes. The nuclear sequences alone are insufficiently variable to fully resolve all lineages and the phylogeny suggests that nuclear gene swapping and incomplete lineage sorting have occurred implying recent divergence. The results from the total evidence analysis provide a phylogenetic hypothesis that both corroborates and complements the previous biogeographic scenario, and provides new insights into the origins of several of the included taxa. PMID:19482093

  16. Evaluation of phytochemical constituents and antioxidant activity of selected actinorhizal fruits growing in the forests of Northeast India

    Indian Academy of Sciences (India)

    Arvind K Goyal; Tanmayee Mishra; Malay Bhattacharya; Pallab Kar; Arnab Sen

    2013-11-01

    Hippophae salicifolia, Elaeagnus pyriformis, Myrica esculenta and M. nagi are actinorhizal plants growing in the sacred forests of Northeast India with multipurpose uses. The present investigation was undertaken to determine the phenol, flavonoid and flavonol contents of the fresh fruit juice of these plant species including the antioxidant potential by means of DPPH, H2O2 and NO scavenging activity and FRP. The total phenolic, flavonoid and flavonol contents of fruit juice ranged from 321.68±0.06 to 76.67±0.01 mg/g GAE, 272.92±0.07 to 20.12±0.02 mg/g QE and 258.92±0.08 to 18.72±0.02 mg/g QE, respectively. At 2.0 mg/mL concentration, DPPH scavenging activity was found to be the highest in M. esculenta (89.62%) and the lowest in E. pyriformis (17.58%). The reducing power activity was found significantly higher in H. salicifolia juice, which increased with increase in concentration. The H2O2 scavenging activity of H. salicifolia juice was found to be as high as 98.78%, while Elaeagnus juice was found to be less effective with just 48.90%. Juice of H. salicifolia showed the greatest NO scavenging effect of 75.24% as compared to juice of E. pyriformis, where only 37.54% scavenging was observed at the same concentration. Taking into account all the experimental data, it can be said that the fruits of H. salicifolia and both M. nagi and M. esculenta have good antioxidant activity compared to fruits of E. pyriformis.

  17. A systematic review of anti-obesity medicinal plants - an update.

    Science.gov (United States)

    Hasani-Ranjbar, Shirin; Jouyandeh, Zahra; Abdollahi, Mohammad

    2013-01-01

    Obesity is the most prevalent health problem affecting all age groups, and leads to many complications in the form of chronic heart disease, diabetes mellitus Type 2 and stroke. A systematic review about safety and efficacy of herbal medicines in the management of obesity in human was carried out by searching bibliographic data bases such as, PubMed, Scopus, Google Scholar, Web of Science, and IranMedex, for studies reported between 30th December 2008 to 23rd April 2012 on human or animals, investigating the beneficial and harmful effects of herbal medicine to treat obesity. Actually we limited our search to such a narrow window of time in order to update our article published before December of 2008. In this update, the search terms were "obesity" and ("herbal medicine" or "plant", "plant medicinal" or "medicine traditional") without narrowing or limiting search items. Publications with available abstracts were reviewed only. Total publications found in the initial search were 651. Total number of publications for review study was 33 by excluding publications related to animals study.Studies with Nigella Sativa, Camellia Sinensis, Crocus Sativus L, Seaweed laminaria Digitata, Xantigen, virgin olive oil, Catechin enriched green tea, Monoselect Camellia, Oolong tea, Yacon syrup, Irvingia Gabonensi, Weighlevel, RCM-104 compound of Camellia Sinensis, Pistachio, Psyllium fibre, black Chinese tea, sea buckthorn and bilberries show significant decreases in body weight. Only, alginate-based brown seaweed and Laminaria Digitata caused an abdominal bloating and upper respiratory tract infection as the side effect in the trial group. No other significant adverse effects were reported in all 33 trials included in this article.In conclusion, Nigella Sativa, Camellia Synensis, Green Tea, and Black Chinese Tea seem to have satisfactory anti-obesity effects. The effect size of these medicinal plants is a critical point that should be considered for interpretation. Although there

  18. Land Desertification and it’s Control in Gonghe Basin of Qinghai Plateau, China

    Science.gov (United States)

    Zhang, D.; Gao, S.; Lu, R.

    2009-12-01

    seeding > Caragana direct seeding, Tamarix cutting and seedling > Salix cheilophila deep planting, Sea-buckthorn seedling > Tamarix deep planting > Tamarix seedling > Poplar deep planting > Salix cheilophila seedling > Poplar seedling. It has resolved the key problem of control sand flow speed and low efficiency, sand burying and wind erosion and low conservation rate for forestation in the sandy area.

  19. Host plant shifts and transitions into new adaptive zones in leafhoppers: the example of Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) of Russia and adjacent countries.

    Science.gov (United States)

    Tishechkin, Dmitri Yu

    2016-06-08

    The modes of diversification of Palaearctic Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) are reconstructed based on data on their host plants and distribution in Russia and the adjacent territories. Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) is originally an Oriental group, which penetrated into the Palaearctic from Southeast Asia. The genus Pediopsoides and species of the genus Macropsis that feed on East Asian oaks have not dispersed beyond broadleaf forests of the Eastern Palaearctic. Apparently, Pediopsis and elm-feeding species of Macropsis initially dispersed throughout the entire broadleaf forest zone. Division of this zone into two widely separated parts in temperate areas of Europe and East Asia (nemoral disjunction), produced closely related vicariant pairs of sister species. The genus Oncopsis and species of Macropsis feeding on Salicaceae dispersed throughout the entire Palaearctic following their host plants. Both lineages penetrated into riparian forests of the foothills and midlands of Central Asia, where they produced endemic species. The Central Asian Macropsis lineage shifted from Salicaceae to trees and shrubs of unrelated families (wild roses, barberry, oleaster, and sea-buckthorn) growing in the same biotopes. Subsequent diversification on those plants produced several separate host-associated species-groups, some of which penetrated following their hosts from riparian forests into arid habitats. One such lineage apparently shifted from shrubs to wormwood species (Artemisia spp.) and thus gave rise to the genus Macropsidius. This genus underwent adaptive radiation on wormwood species in the plains of South Kazakhstan and Central Asia; advancing westward, it formed secondary centres of diversity in Transcaucasia and the Mediterranean. Finally, some lineage of Macropsidius (or its sister-group) switched from feeding on Artemisia to polyphagy, yielding the ancestral form of the genus Hephathus. In general, the evolution of

  20. Host plant shifts and transitions into new adaptive zones in leafhoppers: the example of Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) of Russia and adjacent countries.

    Science.gov (United States)

    Tishechkin, Dmitri Yu

    2016-01-01

    The modes of diversification of Palaearctic Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) are reconstructed based on data on their host plants and distribution in Russia and the adjacent territories. Macropsinae (Homoptera: Auchenorrhyncha: Cicadellidae) is originally an Oriental group, which penetrated into the Palaearctic from Southeast Asia. The genus Pediopsoides and species of the genus Macropsis that feed on East Asian oaks have not dispersed beyond broadleaf forests of the Eastern Palaearctic. Apparently, Pediopsis and elm-feeding species of Macropsis initially dispersed throughout the entire broadleaf forest zone. Division of this zone into two widely separated parts in temperate areas of Europe and East Asia (nemoral disjunction), produced closely related vicariant pairs of sister species. The genus Oncopsis and species of Macropsis feeding on Salicaceae dispersed throughout the entire Palaearctic following their host plants. Both lineages penetrated into riparian forests of the foothills and midlands of Central Asia, where they produced endemic species. The Central Asian Macropsis lineage shifted from Salicaceae to trees and shrubs of unrelated families (wild roses, barberry, oleaster, and sea-buckthorn) growing in the same biotopes. Subsequent diversification on those plants produced several separate host-associated species-groups, some of which penetrated following their hosts from riparian forests into arid habitats. One such lineage apparently shifted from shrubs to wormwood species (Artemisia spp.) and thus gave rise to the genus Macropsidius. This genus underwent adaptive radiation on wormwood species in the plains of South Kazakhstan and Central Asia; advancing westward, it formed secondary centres of diversity in Transcaucasia and the Mediterranean. Finally, some lineage of Macropsidius (or its sister-group) switched from feeding on Artemisia to polyphagy, yielding the ancestral form of the genus Hephathus. In general, the evolution of

  1. Technology of development phytogenetic protectors and creation of raw material base

    International Nuclear Information System (INIS)

    Recently in order to protect organisms from the of radiation effect radio protectors have been applied principally concerning to sulfur compounds, aminothioles and indolilalkins. Although the achievements obtained in the direction of synthetic medicines discovery, they can not completely replace the medicinal preparation of natural origin. That is why the study of natural biological active substances with radio protector property are of special importance. One of the goals of the project is the detection of plant species rich with anti mutagen and radio protector, the study of their cultivation technology, and the produce of natural raw material with high quality in planting condition. Flora of Azerbaijan is considered one of the richest countries for its species' richness. There are plant varieties for producing radio protector substances (essence-oil, lipid, lipoid, phenol compounds, pigment substances, proteins, vitamins and various amino acids). It was cultivated new varieties and forms of sea buckthorn, black caraway, basil, tarragons which are richer with their radio protector substances among these plants. In the article it is intended to prepare aerosol, consisted of essence oils and lipid mixtures, for respiratory system treatment in extreme conditions having radiation threat. The plants with essence oil are potential radio protectors. On industry scale the researches will be conducted in the direction of this technology realization. There are also some achievements in the field of working out of production technology of food products, with polyvitamins, from plants for extreme conditions and it will be improved in the process of project activies. Scientific importance of alcoholic and less alcoholic drinks in liquidating the impact of radioactive substances in organism and their disposal out of the organism have been found in literatures. It is also intended to conduct research works on production technology of medicinal preparations, liquor, alcohol of

  2. Impact of subjacent rocks at the water and air regime of the depleted peat deposits

    Science.gov (United States)

    Rakovich, V. A.

    2009-04-01

    At the depleted peat deposits (after peat extraction), where the residual layer of peat with the thickness of about 0,5 meters is laid at the well water permeable rocks, vegetation typical for dry conditions is developed in case of good drainage conditions; birch trees, willow, alder-trees and buckthorn prevail in this vegetation. Water and air regime is characterized here by good aeration with prevailing of oxidative processes. If water regime is regulated, these depleted peat areas are suitable for agricultural and forest lands; however, necessity of transformation of these depleted lands into forest and agricultural lands must be ecologically and economically justified. If the residual layer of peat with the thickness of 0,05-0,3 m is based at the sapropel or peat sapropel, contrast amphibiotic water and air regime with strong fluctuation of oxidative and restoration process depending on the weather conditions is formed; this regime is formed without artificial increase of the ground waters level. This does not allow bog vegetation or vegetation typical for dry conditions to develop. Thus, within 20 and more years after completion of peat extraction, such areas are not covered by vegetation in spite of favorable agro-chemical qualities of peat layer and favorable for vegetation chemical composition of soil and ground waters. Depleted peat deposits, that are based at the sapropel, are not suitable for agricultural use, because agricultural vegetation requires stable water and air regime with good aeration and oxidative and restoration potential within 400-750 mV. Contrast amphibiotic water and air regime of the depleted peat deposits that are based at sapropel excludes possibility to use them as agricultural lands. Because of this reason, areas with residual peat layer that are based at sapropel are not suitable for forest planting. Due to periodic increase of ground waters level, rot systems of the plants can not penetrate into the required depth, and mechanical

  3. Determination of Cd in Seabuckthorn Vitamin P Powder by Graphite Furnace Atomic Absorption Spectrometry Using Solid Sampling%固体进样—石墨炉原子吸收法测定沙棘维生素P粉中镉含量的研究

    Institute of Scientific and Technical Information of China (English)

    张荣; 姬丁坤; 杨英桂

    2012-01-01

    Direct solid sampling graphite furnace atomic absorption spectrometry(SS-GF AAS) was used to determine Cd in Vitamin P sea-buckthorn powder.After the program optimization and with an extremely careful selection of chemical modifier,the stable and accurate results could be obtained.The same results could finally be carried out by ICPMS using liquid sampling.The RSD was between 6.08%~11.42%,the recovery rate was between 90.08%~113.63%,the detection limit was 0.0034ng.Compared with liquid sampling graphite furnace AAS analysis,the Direct SS-GF AAS proved to be a good alternative to conventional methods of trace analysis after wet-chemical digestion and compared favorably with these methods.It can save a lot of work for sample processing,has benefits to avoid dilution and external contamination.%本研究采用直接固体进样-石墨炉原子吸收法(SS-GF AAS)测定沙棘维生素P粉中重金属镉的含量。通过对石墨炉工作程序的优化,同时采用了基体改进剂技术,最终得到稳定而准确的结果,其结果与液体进样使用ICP-MS测量结果一致。本方法中RSD在6.08%~11.42%之间,回收率在90.75%~105.63%之间,检出限为0.0034ng。与湿法消解方法相比较,该方法无需样品前处理,具有简便、快速特点,同时可避免样品的稀释以及试剂的交叉污染带来的分析误差。

  4. Isolation, identification and application of special bioactive plant components

    International Nuclear Information System (INIS)

    Complete text of publication follows. The wide application of natural and bioactive compounds has became a central issue in the last years. The use of these materials as food additives can be advantageous in several aspects: their natural origin, health protecting properties, possibility for combination of beneficial physiological properties and the recognition and exploitation of synergetic properties can be used in the production of wide range of functional food products. The main objective of the work is - to compare the efficiency of extraction procedures used to obtain different bioactive compounds from different plants, - to identify bioactive components, - to examine the biological activity and bioavailability of the isolated compounds, - to separate and purify the active substances, - to examine the possibility to use these components as functional food additives, - (optimal retrieval, stability, interactions with the food matrix, bio-accessibility). We have examined carrot, purple corn, red cabbage, red pepper, garlic and sea buckthorn extracts. The extraction was carried out with seven different solvents (ethanol: acetic acid:water, acetone, ethanol, n-hexane, ethyl-acetate and hot water). The analysis of the different extraction methods showed that hot water and ethanol were the most efficient solvents, while ethyl-acetate and n-hexane were less effective. The antioxidant activities were determined by FRAP and DPPH methods, antioxidant activities were found to be high in case of garlic and purple corn, while carrot showed lower activity. The components of the extracts were identified by HPLC-MS technique; three unique components were identified in carrot, eleven components in purple corn and nine components in red cabbage. The expected bioavailability and utilization of the components were determined with the application of digestion models: 1 gram of the sample was shaken for 4 hours with 25 ml 0.01 M NaCl solution containing 1 gram pepsin at pH=2.0 in

  5. Biodiversity of medicinal plants in north east India: their systematic utilisation

    Directory of Open Access Journals (Sweden)

    Rama Shankar

    2011-01-01

    Full Text Available (Abstract selected from presentation in National Conference on Biodiversity of Medicinal and Aromatic Plants: Collection, Characterization and Utilization, held at Anand, India during November 24-25, 2010 North eastern India comprising of eight states namely Assam, Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, Sikkim and Tripura is widely rich in biodiversity belonging to different climatic conditions varying from tropical subtropical, temperate and alpine zones which covers the major occurrence of medicinal plants. The different states of North east India are falling into different hill zones like Eastern Himalaya beginning from Sikkim to Lohit district of Arunachal Pradesh, Naga hills covering the areas of Nagaland and Manipur states, Lusai hills with Mizoram and Tripura states and Garo, Jayantiya and Khasi hills occupying the state of Meghalaya. The biodiversity distribution extents even to neighbouring countries like China, Mayayanmar and Bangladesh. Classification of different hills of north eastern India provides the distribution of specific medicinal plants like Sikkim is the richest source for Berginia ciliate, Cordiceps sinensis, Hippophae species, Nardostachys jatamansi, Picrorrhiza kurrooa, Swertia chirayita; Arunachal Pradesh for Acorus calamus, Aquillaria agallocha,  Berberis aristata, Cinnamomum tamala, C. zeylanica, Coptis teeta, Cordiceps sinensis, Embelia ribes, Paris polyphylla, Rubia cordifolia, Gynocordia odorata, Illicium griffithii, Taxus wallichiana, Valeriana jatamansi; Nagaland and Manipur for Smilax glabra, Asparagus adscendens with less occurrence of Rubia cordifolia, Taxus wallichiana, Thallictrum foliolosum etc; Jayantiya hill of Meghalaya comprises of wider distribution of Embelia ribes; Lusai hill covering the state of Mizoram and eastern plains of Tripura comprises of Holorhena antidysenterica, Mallotus phillippinensis, Saraca asoka, Smilax glabra, Stephania glabra and Terminalia chebula. Plants

  6. Effects of invasive plant species on pollinator service and reproduction in native plants at Acadia National Park

    Science.gov (United States)

    Stubbs, C.J.; Drummond, F.; Ginsberg, H.

    2007-01-01

    Invasive plant species can have profound negative effects on natural communities by competively excluding native species. Berberis thunbergii (Japanese barberry), Frangula alnus (glossy or alder buckthorn) and Lythrum salicaria (purple loosestrife) are invasive species known to reduce native plant diversity and are thus of great concern to Acadia National Park. Pollinators visit them for nectar and pollen. The effects of invasive plant species on pollinator behavior were investigated by comparing pollinator visitation to co-flowering native and invasive species with visitation to native species growing alone. The effect of invasives on pollination of native plants was studied by comparing fruit set in patches of the native species growing near invasives with patches far from invasive species in Acadia National Park. The coflowering pairs were as follows: in the spring native Vaccinium angustifolium (lowbush blueberry) was paired with B. thunbergii; in early summer native Viburnum nudum (wild raisin) was paired with F. alnus ; in late summer native Spiraea alba (meadowsweet) was paired with L. salicaria. We investigated whether these invasives competed with native plants for pollinators in Acadia and thus negatively affected native plant reproduction. Our objectives were to determine: 1) the influence, if any, of each invasive on pollinator visitation to a co-flowering native species, 2) factors that might affect visitation, 3) invasive pollen transfer to native plants, and 4) whether invasives influence native plant reproduction (fruit set). Our findings indicate that at times the number of flower visitors to natives was lower or the species composition of visitors different when invasives were present, that invasives sometimes attracted more pollinators, that generally the invasives were more rewarding as far as nectar and pollen availability for pollinators, and that generally native plant fruit set and seed set was not significantly lowered in the presence of

  7. 沙棘种子萌发期过氧化物酶活性检测%Detect of POD Activity in HipopopHae Seed Germination Phase

    Institute of Scientific and Technical Information of China (English)

    何士敏

    2012-01-01

    沙棘是一种生命力极强的灌木,一般生长在干燥、寒冷的贫瘠山区.沙棘果实和叶片中含有丰富的生物活性成分,是珍贵的药食两用植物资源.同时沙棘还对防止水土流失、改善生态环境,具有十分重要的作用.对沙棘进行高层次的综合开发利用具有重要意义.过氧化物酶(POD)作为植物细胞内保护酶系统的一种,与植物抵御极端温度、干旱、重金属胁迫、病害等各种不良环境有着密切的关系.本文研究了在不同pH、温度条传下,沙棘种子萌发期过氧化物酶的稳定性.结果表明,沙棘种子萌发期过氧化物酶的pH值稳定性和热稳定性都比较低,其作用最适pH值为6.2,最适温度为35℃.同时,实验还检测了底物浓度对沙棘种子萌发期过氧化物酶活性的影响,其最适底物浓度为0.06 mol/L.%The Seabuckthorn is a kind of bush with great vitality and it often grows in the dry and cold barren mountain areas. The fruit and the leaves which contain the active coposition of rich living things are the valuable plant resources with medicine and food for dual purpose, besides, it plays a very important role in guarding against soil erosion and improving the ecological environment. Therefore,a high-level comprehensive development on Sea buckthorn has great significance. Peroxidase (POD) as one of the enzyme with protection enzyme system in the cells of plant, which has close relation with plans withstanding extreme temperatures, drought, heavy metal stress, disease and other adverse environment. This study discussed the stability of peroxidase extracted from the Seabuckthorn leaves at different pH or temperatures. The results showed that the peroxidase from the HipopopHae bud had not better thermally stability and pH stability, the optimal pH was 6. 2 and the optimal temperature was 35 ℃. Meanwhile, this experiment also detected the influence that the substrate concentration to peroxidase activity. Its

  8. 刺槐与其他树种枯落叶混合分解对微量元素释放的影响%Influences of mixed leaf litter decomposition of Robinia pseudoacacia with other trees on microelements release

    Institute of Scientific and Technical Information of China (English)

    杜良贞; 刘增文; 祝振华; 李茜

    2012-01-01

    whole Loess Plateau, through collecting leaf litter of R. pseudoacacia and other 10 trees at the end of autumn,cutting the leaf to some fragments (a=l cm) or 1 cm needles,and gathering humus soil (0-20 cm) of no forested land as medium,the incubation of mixed leaf litter decomposition had been carried out for 345 d with the method of nylon mesh bag buried with soil in the same condition of tem- perature and moisture. Then,the microelements (Cu,Zn,Ni,Cd) contents before and after leaf litter de-composition were determinated with the Atomic Absorption Spectroscopy(AAS)method. [Result] The re- lease rates of Cu,Zn,Ni and Cd during unique leaf litter decomposition of Populus simonii ,Hippophae rh- amnoides ,Platycladus orientalis and Quercus liaotungensis are all the fastest and the turnover period is 1 year or a little more. Mixing litter decomposition of R. pseudoacacia with P. simonii,Ulmus pumila, H. rh- amnoides separately would produce promoted actions on release of the microelements, whereas mixed litter decomposition of R. pseudoacacia with Pinus sylvestris var. mongolica, P. orientalis, Betula platyphylla and Caragana microphylla separately would produce inhibitory actions on release of the 4 microelements. Mixed litter decomposition of R. pseudoacacia with Pinus tabulaeformis and Larix principis-rupprechtii separately would promote release of Cu, but inhibit releases of Zn, Ni and Cd. Mixed litter decomposition of R. pseudoacacia with Q. liaotungensis would promote releases of Zn and Cd,but inhibit releases of Cu and Ni. [Conclusion] Mixed forestation of R. pseudoacacia with other trees which promote release of microele- ments after mixed litter decomposition would be helpful for the microelement cycling and utilization in for- estland.

  9. 黄土丘陵沟壑区坡面尺度土壤水分空间变异及影响因子%Spatial patterns of soil moisture at transect scale in the Loess Plateau of China

    Institute of Scientific and Technical Information of China (English)

    姚雪玲; 傅伯杰; 吕一河

    2012-01-01

    Understanding the spatial variability and impact factors of soil moisture is important when modeling the antecedent soil moisture status of a catchment. Soil moisture is a limiting factor of ecosystem development in semi-arid and arid areas. Soil moisture spatial patterns and impact factors are particularly important for ecosystem restoration in the semi-arid areas of the Loess Plateau. This study identifies soil moisture spatial patterns and impact factors (terrain, land use types and wet season) in Yangjuangou catchment in north of the Loess Plateau from the measurement of soil moisture in the top 100 cm soil layer along five different transects on a loess hill before and after the wet season. Statistical analysis revealed that the soil moisture spatial variation in deeper soil ( > 40cm) was more significant than that in upper soil ( planted R. pseudoacacia forest land (8 years) > planted R. pseudoacacia forest land (20 years) > planted Hippophae reamnoides forest land, no matter before or after the wet season. Although the topography of planted forest land has advantageous conditions (shading slope, lower position, smaller gradient or terraces) for retaining soil moisture, the soil in planted land was still found to be have a much lower soil moisture content than the soil in natural grasslands, especially in deeper soil. Thus it is considered that the influence of terrain on soil moisture content is masked by the influence of surface vegetation. Therefore, when modeling hydrological processes in the Loess Plateau, it is recommended that the pattern of vegetation types within the simulated area should be paid special attention. Hydrologic models of the Loess Plateau based only on terrain properties should be used cautiously. The soil moisture content at all sample sites increased after the wet season, however, the spatial patterns of soil moisture was not significantly changed. Sites which had high soil moisture content relative to other sites before the wet