WorldWideScience

Sample records for brooming

  1. Teaching Near and Far - Broome, Western Australia.

    Science.gov (United States)

    Herceg, Christina; Renouf, Tia

    2016-11-09

    Broome is a remote coastal town in Western Australia. As a general practitioner working in Broome, I have been involved in the education of general practice trainee registrars both locally and remotely, as a supervisor with two different training programs.

  2. Synthesis and characterization of lower generation broom molecules

    Institute of Scientific and Technical Information of China (English)

    Jun Wang; Cui Qin Li; Shu Yan Zhang; Fang Sun; Teng Jie Ge

    2008-01-01

    Dendritic molecules with dodecyl groups as the hyperbranchs were synthesized in methanol by Michael addition withdodecylamine and methyl acrylate as raw materials. This new-type dendritic molecules were called vividly "broom molecules" inthis report. The surface tension of the aqueous solution of broom molecule terminated amino group was measured by using the drop-volume method. The demulsification performance of the broom molecules for the oil/water (O/W) simulated crude oil emulsion wasexamined. The experimental results revealed that, as a new-type of surfactants, the broom molecules terminated amino groupsshowed demulsification for the O/W simulated crude oil emulsion.

  3. Extraction Methods of Spanish Broom (Spartium Junceum L.

    Directory of Open Access Journals (Sweden)

    Drago Katović

    2011-12-01

    Full Text Available Effects of different extraction methods of the Spanish Broom shoots were measured and compared with the purpose of obtaining composite material. The content of cellulose, lignin, pentosan and ash in the Spanish Broom fibers was determined. SEM analyses were performed.

  4. Experimental control of Spanish broom (Spartium junceum invading natural grasslands

    Directory of Open Access Journals (Sweden)

    Cristina Sanhueza

    2012-12-01

    Full Text Available A group of legumes generically known as brooms are among the most successful shrubs invading grasslands in South America and otherregions. These species share a set of biological features that enhance their invasiveness, such as abundant and long-lasting seed banks,aggressive root systems and rapid growth, combined with their ability for re-sprouting after cutting or burning and for avoiding herbivores.They grow in dense stands that exclude native vegetation and are able to change ecological processes, increasing fire frequency and intensity,and fixing atmospheric nitrogen. The Spanish broom (Spartium junceum is a shrub native form the Mediterranean that was introduced intothe Argentine Pampas grasslands where it spreads over remnants of pristine ecosystems, threatening their biodiversity. This paper reports theresults obtained after an adaptive management strategy aimed at controlling this species in a nature reserve, and compares the efficiency ofdifferent mechanical and chemical control techniques in terms of the number of plants killed and the effects on surrounding vegetation andon the recruitment of broom seedlings. Control was implemented in two phases, the first included three treatments: i cut at the base of theplant, ii cut followed by the immediate application of Togar (Picloram 3% + Triclopyr 6%, at a 5% dilution in diesel oil on top of the cut stump, and iii foliar spraying with Togar. The follow-up treatments, implemented one year later, consisted of spraying the re-sprouts with Togar (5% in diesel oil or Glyphosate 36% (2% in water. The best option in terms of controlling Spanish broom was spraying the resprouts with Togar which gave 100% mortality of the treated plants, compared with values of 40% - 100% re-sprouting for the other optionstested. None of the methods was associated with an increase in seedling recruitment, nor with significant changes in the vegetation in the immediate vicinity of the controlled brooms.

  5. Restoration of Hyperspectral Push-Broom Scanner Data

    DEFF Research Database (Denmark)

    Larsen, Rasmus; Nielsen, Allan Aasbjerg; Conradsen, Knut

    1997-01-01

    Several effects combine to distort the multispectral data that are obtained from push-broom scanners. We develop an algorithm for restoration of such data, illustrated on images from the ROSIS scanner. In push-broom scanners variation between elements in the detector array results in a strong...... striping along flight lines. A non-systematic striping is also present along flight lines. Furthermore, line drop-outs occur, and finally, various types of electronic noise of salt-and-pepper type are also present. We describe techniques for the correction for all these types of effects. Line drop...... back into the original spectral space results in noise corrected variables. The noise components will now have been removed from the entire original data set by working on a smaller set of noise contaminated transformed variables only. The application of the above techniques results in a dramatic...

  6. Broom fibre PRB for heavy metals groundwater remediation

    Science.gov (United States)

    Molinari, A.; Troisi, S.; Fallico, C.; Paparella, A.; Straface, S.

    2009-04-01

    human being. One of the most interesting techniques applied in contaminated aquifer by heavy metals is the PRBs (Troisi et al., 2002; Calvin et al., 2006), in particular broom fibers PRB (Troisi et al., 2008). The first results highlight an optimum removal capacity for contaminants underlined from following removal percentage: 98.01% (Cd), 99.95% (Cu), 97.35% (Pb) and 99.53% (Zn). A fundamental parameter for PRB design is the decay coefficient who indicates the removal capacity (degradation, transformation, adsorption/absorption, mass transport, etc.). This parameter has been determined for four heavy metals: Cadmium (Cd), Copper (Cu), Lead (Pb) and Zinc (Zn) carrying out column tests. Besides, for real use of broom fibers PRB same tests have been performed, using flow cells, to estimate a relation between hydraulic conductivity of fiber and its density. References Chien C. C., H. I. Inyang and L.G. Everett (2006). Barrier Systems for Environmental Contaminant Containment and Treatment. Taylor and Francis Group eds. Troisi S., C. Fallico, S. Straface S. e L. Mazzuca. (2008). Biodreni per la bonifica di siti contaminati realizzati con fibre naturali liberiane ad elevato sviluppo superficiale. CS2008A00018. Università della Calabria. Troisi S, E. Migliari and S. Straface (2002). Soil and groundwater contamination by heavy metals in the industrial area of Crotone. Third International Conference Risk Analysis III. Sintra, Ed. by C.A. Brebbia. WIT Press.

  7. Design of a Push-Broom Multi-Beam Radiometer for Future Ocean Observations

    DEFF Research Database (Denmark)

    Cappellin, C.; Pontoppidan, K.; Nielsen, P. H.

    2015-01-01

    The design of a push-broom multi-beam radiometer for future ocean observations is described. The radiometer provides a sensitivity one order of magnitude higher than a traditional conical scanning radiometer, and has the big advantage of being fully stationary relative to the satellite platform...

  8. Characterization of broom fibers for PRB in the remediation of aquifers contaminated by heavy metals

    Directory of Open Access Journals (Sweden)

    C. Fallico

    2010-08-01

    Full Text Available The present level of pollution, increasingly involving ground waters, constitutes a serious risk to the environment and also to human health. Therefore the remediation of saturated and unsaturated soils to remove pollutant materials is more and more frequently required. In the present paper, the possibility of removing heavy metals by permeable reactive barrier (PRB from the groundwater carried out specifically with broom fibers, is investigated.

    Once shown the economic benefits deriving from the use of this plant, a hydraulic characterization of the broom fiber mass was performed, determining the permeability and the porosity in correspondence to different levels of compactness of the fibers.

    Having verified the effectiveness of removal of some heavy metals by these fibers, the results of some experiments, carried out in the laboratory for this purpose, are shown. These experiments were carried out utilizing broom fibers obtained in different ways and, limitedly to the considered pollutants, showed the high capability of these fibers to reduce their concentrations. The best results were obtained for the broom fibers extracted by a particular chemical-physical process.

    Moreover, the behaviour of this fiber with time was investigated, determining the kinetic constant of degradation.

  9. Case of foreign body as a broom handle in the rectum

    Directory of Open Access Journals (Sweden)

    Vikash Lal

    2014-08-01

    Full Text Available A 68 yrs old male patient with a foreign body (broom handle cover 11'inch introduced as sexual perversion presented with lower abdominal pain, the management emphasis is a transanally retrieval and ruling out of rectal and colonic perforation under colonoscopy guidance under local anesthesia in pad. [Int J Res Med Sci 2014; 2(4.000: 1761-1762

  10. Characterization of broom fibers for PRB in the remediation of aquifers contaminated by heavy metals

    Directory of Open Access Journals (Sweden)

    C. Fallico

    2010-02-01

    Full Text Available The present level of pollution, increasingly involving ground waters, constitutes a serious risk for the environment and also for human health. Therefore the remediation of saturated and unsaturated soils to remove the pollutant materials is required more and more frequently. In the present paper, the possibility of removing the specific pollutant typology constituted of heavy metals by permeable reactive barrier (PRB from the groundwater, carried out with natural fibers, specifically with broom fibers, is investigated.

    Once shown the economic benefits deriving from the use of this plant, a hydraulic characterization of the broom fiber mass was performed, determining the permeability and the porosity in correspondence to different levels of compactness of the fibers.

    Having verified the effectiveness of these fibers for some heavy metals, the results of some experiments, carried out in the laboratory for this purpose, are shown. These experiments were carried out utilizing broom fibers obtained in different ways and, limitedly to the considered pollutants, showed the high capability of these fibers to reduce their concentrations. The best results were obtained for the broom fibers extracted by a particular chemical-physical process.

    Moreover, the behaviour of this fiber with time was investigated, determining the kinetic constant of degradation.

  11. Hypersensitivity pneumonitis caused by the broom grass (Calluna vulgaris

    Directory of Open Access Journals (Sweden)

    Yusuf Aydemir

    2015-01-01

    Full Text Available Hypersensitivity pneumonitis (HP, is an inflammatory disease that represents one possible response of the interstitial and parenchymal tissue to the intensive and repeated inhalation of organic dusts or reactive chemicals substances. In this case report, for the first time in the literature as we know, acute hypersensitivity pneumonitis in a patient who working in broom grass manufacturing were presented. Broom manufacturer employee 35 years old female patient was admitted with dyspnea, fever and cough, beginning the three months before. Physical examination, there were bilateral basal crackles. On Thorax CT bilateral diffuse ground-glass opacity and mosaic perfusion were observed. Patient was hospitalized, IV prednisolone therapy was started. Almost complete clinical improvement was achieved. We would like to emphasize that detailed occupational history should be questioned; because; diagnosis of acute HP, due to the mixing with the viral infection or atypical pneumonia, easily skipped.

  12. Genetic molecular diversity, production and resistance to witches’ broom in cacao clones

    Directory of Open Access Journals (Sweden)

    José Luis Pires

    2013-06-01

    Full Text Available The 32 cacao clones selected as being resistant following the witches’ broom epidemic and for having distinct productivitywere characterized according to their genetic diversity and were submitted to a new selection. These plants were assessed for eightyears at the Oceania Farm (FO in Itagibá, Bahia, Brazil. The 13 microsatellite primers generated an average of 11.7 amplicons perlocus, and based on them it was demonstrated that the 32 clones distribute themselves in groups apart from the nine clones used ascontrols. The 32 materials displayed significant differences in relation to the characters assessed in the field. Two criteria were formedfrom the classification of the most productive and resistant plants, and then used to select plants within the clusters. The selected plantsdisplayed potential for the cacao improvement program, that they have a high production and high resistance to witches’ broom.

  13. RHIZOSPHERE pH AND PHOSPHATASE ACTIVITY IN ORTHIC ALLOPHANIC SOIL UNDER Pinus radiata SEEDLINGS GROWN WITH BROOM AND RYEGRASS

    Directory of Open Access Journals (Sweden)

    Achmad A. Rivaie

    2009-06-01

    Full Text Available Under  Pinus radiata plantations  where  the tree spacing  is wider  and most soils are phosphorus  (P deficient,  the radiata  tree response to P fertilizer is expected  to be more influenced  by  the interaction between  the applied  P fertilizer, the tree and understorey vegetation.  Therefore,  a better understanding of the soil P chemistry under radiata pine trees in association  with  other  plants  is required.  We investigated  the effect of broom  (Cytisus scoparius L. and ryegrass  (Lolium multiflorum grown  with  radiata  seedlings  in Orthic Allophanic Soil treated with  0, 50, and 100 μg P g-1  soil of TSP on the pH and phosphatase activity in the rhizosphere soils under glasshouse condition. The pHs of radiata rhizosphere soils either grown with broom or grass were lower than  those in the  bulk soils and the bulk and rhizosphere soils of grass and broom,  whether  they  were grown  alone or grown  with radiata at the  applications of 50 and 100 μg P g-1 soil. These results suggest that P application enhanced root induced acidification  in a P-deficient Allophanic Soil under radiata.  The soils in the rhizosphere of grass and broom, grown in association with radiata, were also acidified by  the effect of radiata  roots.  Acid  phosphatase  activity in soils under  radiata,  grass and broom  decreased with  an increased  rate of P application. At all P rates,  acid phosphatase activity was higher in the rhizosphere of radiata  grown  with  broom than in the bulk soils. The phosphatase activity in the rhizosphere soil of radiata grown with broom was also higher than that of radiata grown with grass, but it was slightly lower than that in the rhizosphere of broom grown  alone. These results suggest that broom may have also contributed to the higher  phosphatase  activity in the rhizosphere soils than  in the bulk  soils of broom  and radiata when they were grown  together

  14. Apoplastic and intracellular plant sugars regulate developmental transitions in witches’ broom disease of cacao

    OpenAIRE

    Barau, Joan; GRANDIS, Adriana; Carvalho, Vinicius Miessler de Andrade; Teixeira, Gleidson Silva; Zaparoli, Gustavo Henrique Alcalá; do Rio, Maria Carolina Scatolin; Rincones, Johana; Buckeridge, Marcos Silveira; Pereira, Gonçalo Amarante Guimarães

    2014-01-01

    Witches’ broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant–fungus interface, the apoplastic fluid of cacao. Data are provided supporting a role for the dynamics of soluble carbon in the apoplastic fluid in prompting the end of the biotrophic phase of infection. Carbon deple...

  15. Development of Abrasive Selection Model/Chart for Palm Frond Broom Peeling Machine Design

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    Nwankwojike

    2014-12-01

    Full Text Available A model for predicting the friction required by a palm frond broom peeling machine for effective peeling of palm leaf to broom bristle and a chart for selecting the best abrasive material for this machine’s peeling operation were developed in this study using mechanistic modeling method. The model quantified the relationship between the coefficient of friction and other operational parameters of this machine while the abrasives selection chart constitutes a plot of this measured friction parameter against the abrasive materials used in palm frond broom peeling machine fabrication. The values of the coefficient of friction of palm leaf on different abrasive materials used in this plot were determined from experimental study of the effect of moisture content level of naturally withered palm leaves (uninfluenced by external forces on their coefficient of friction with the abrasives. Results revealed the average moisture content of palm leaf this machine can peel effectively as 6.96% and also that the roughest among the abrasives that approximate the coefficient of friction for a specific design of this peeling machine gives maximum peeling efficiency. Thus, the roughest among the abrasive materials that approximate the coefficient of friction for a specific design of this machine should be selected and used for its fabrication and operation.

  16. Dr. Reggie J. Laird Broom: pionero de la ciencia del suelo en México

    Directory of Open Access Journals (Sweden)

    David Espinosa Victoria

    2017-01-01

    Full Text Available A casi tres años de su deceso, la Sociedad Mexicana de la Ciencia del Suelo, A.C. rinde, a través de esta semblanza, un homenaje póstumo a la memoria de uno de los pioneros de la Ciencia del Suelo en México. El trabajo realizado por el Dr. Reggie J. Laird Broom (Figura 1, a lo largo de 45 años, refleja de forma contundente su compromiso y pasión por ayudar a los pequeños productores de México, América Latina y el mundo.

  17. Comparative performance studies between tunable filter and push-broom chemical imaging systems

    Science.gov (United States)

    Malinen, Jouko; Saari, Heikki; Kemeny, Gabor; Shi, Zhenqi; Anderson, Carl

    2010-04-01

    This paper reports instrument characterization measurements, which were recently arranged to provide comparative information on different hyperspectral chemical imaging systems. Three different instruments were studied covering both tunable filter and push-broom techniques: The first instrument MatrixNIRTM is based on a LCTF tunable filter and InGaAs camera and covers wavelengths from 1000 to 1700 nm. The second one SisuCHEMATM is based on push-broom technology and MCT camera operating from 1000 to 2500 nm. The third system is an instrument prototype from VTT Technical Research Centre of Finland exploiting high speed Fabry-Perot interferometer and MCT camera, currently calibrated from 1260 to 2500 nm. The characterization procedure was designed to study instrumental noise, signal-to-noise ratio, linearity and spectral as well as spatial resolution. Finally, a pharmaceutical tablet sample was measured with each instrument to demonstrate speed of measurement in a typical application. In spite of differences in wavelength ranges and camera technologies used, the results provide interesting information on relative instrumental advantages and disadvantages, which may be useful for selecting appropriate instrumentation for defined applications. Further, an additional aim of this study is to compare the high speed Fabry-Perot imaging technology under development against the established chemical imaging techniques available on the market today.

  18. A potential role for an extracellular methanol oxidase secreted by Moniliophthora perniciosa in Witches' broom disease in cacao

    Science.gov (United States)

    The hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of witches’ broom disease (WBD) of cacao, is able to grow in methanol as sole carbon source. In plants, one of the main sources of methanol is the pectin present in the structure of cell walls. Pectin is composed b...

  19. A Study of the Relationship between Attendance and Grades of Three Business Law Classes at Broome Community College.

    Science.gov (United States)

    Davenport, William S.

    In an effort to encourage the growth and maturity of its students, Broome Community College in New York abolished its attendance policy. For the past 7 years, both attendance rates and grade point averages (GPA's) of students at the college had been dropping. In 1990, a study was conducted at BCC to determine if there was a measurable relationship…

  20. SIR2012-5282 Surficial Geology: Hydrogeology of the Susquehanna River valley-fill aquifer system and adjacent areas in eastern Broome and southeastern Chenango Counties, New York

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The hydrogeology of the valley-fill aquifer system along a 32-mile reach of the Susquehanna River valley and adjacent areas was evaluated in eastern Broome and...

  1. Identification of candidate genes involved in witches’ broom disease resistance in a segregating mapping population of Theobroma cacao L. in Brazil

    Science.gov (United States)

    Witches’ broom disease (WBD) caused by the fungus Moniliophthora perniciosa is responsible for considerable economic losses for cacao producers in the Americas. Protective fungicides are ineffective, and disease management involving repeated phytosanitary removals increases labor costs. The best al...

  2. Transgenic Paulownia Expressing shiva-1 Gene Has Increased Resistance to Paulownia Witches' Broom Disease

    Institute of Scientific and Technical Information of China (English)

    Tao DU; Yao WANG; Qin-Xue HU; Jie CHEN; Sheng LIU; Wen-Jin HUANG; Mu-Lan LIN

    2005-01-01

    Stem segments from diseased Paulownia tomentosa×P. fortunei and leaves from healthy control were transformed with the expression vector p438PRSI via Agrobacterium tumefaciens. The p438PRSI vector contained shiva-1 gene, which encodes an antibacterial peptide under the control of a CaMV35S promoter. The regenerated plants from transformed explants were planted in a greenhouse and nursery. PCR and Southern blotting analysis showed that the shiva-1 gene was successfully integrated into the Paulownia genome. Transcription of the integrated shiva-1 gene was confirmed by RT-PCR. Bioassay in the green house and phytoplasma DNA-dot blotting demonstrated that resistance to Paulownia witch's broom disease (PWB) increased significantly in shiva-1-transgenic Paulownia. Further investigations indicated that higher Shiva-1 expression correlated with fewer phytoplasma and less symptoms in diseased transgenic Paulownia. Together, our findings strongly suggest that breeding shiva-1-Paulownia is an effective strategy to control PWB disease.

  3. Joint Sandia/NIOSH exercise on aerosol contamination using the BROOM tool.

    Energy Technology Data Exchange (ETDEWEB)

    Ramsey, James L., Jr. (.,; .); Melton, Brad; Finley, Patrick; Brockman, John; Peyton, Chad E.; Tucker, Mark David; Einfeld, Wayne; Brown, Gary Stephen; Griffith, Richard O.; Lucero, Daniel A.; Knowlton, Robert G.; McKenna, Sean Andrew; Ho, Pauline

    2006-06-01

    In February of 2005, a joint exercise involving Sandia National Laboratories (SNL) and the National Institute for Occupational Safety and Health (NIOSH) was conducted in Albuquerque, NM. The SNL participants included the team developing the Building Restoration Operations and Optimization Model (BROOM), a software product developed to expedite sampling and data management activities applicable to facility restoration following a biological contamination event. Integrated data-collection, data-management, and visualization software improve the efficiency of cleanup, minimize facility downtime, and provide a transparent basis for reopening. The exercise was held at an SNL facility, the Coronado Club, a now-closed social club for Sandia employees located on Kirtland Air Force Base. Both NIOSH and SNL had specific objectives for the exercise, and all objectives were met.

  4. Growth response of broom (Cytisus scoparius) growing with and without radiata pine (Pinus radiata) seedlings to different P levels in soils

    Institute of Scientific and Technical Information of China (English)

    Achmad Arivin Rivaie

    2011-01-01

    A study was carried out to test the effects of three rates of TSP (triple superphosphate) (0,50,and 100 mg·kg-1 P) on growth of broom with and without radiata pine seedlings and to determine the relationships between P concentrations in the broom shoot and dry matter yields with soil plant-available P (Bray-2 P).A bulk sample of soil was collected from Kaweka forest at soil depth of 0-10 cm,in New Zealand on March 11,2001.The forest area was not supplied with fertiliser at least 30 years.The results show that TSP application increased P availability in the soil.The P availability concentration in soil of broom with radiata pine seedlings was higher than that in soil of broom alone.Bray-2 P concentrations had a significant logarithmic relationship with P concentrations of broom shoot and an exponential relationship with dry matter weight of whole broom plant.

  5. Additional collection devices used in conjunction with the SurePath Liquid-Based Pap Test broom device do not enhance diagnostic utility

    Directory of Open Access Journals (Sweden)

    O'Connor Jason C

    2004-09-01

    Full Text Available Abstract Background We have previously shown that use of an EC brush device in combination with the Rovers Cervex-Brush (SurePath broom offered no significant improvement in EC recovery. Here we determine if use of additional collection devices enhance the diagnostic utility of the SurePath Pap for gynecologic cytology. Methods After informed consent, 37 women ages 18–56 receiving their routine cervical examinations were randomized into four experimental groups. Each group was first sampled with the SurePath broom then immediately re-sampled with an additional collection device or devices. Group 1: Rover endocervix brush (n = 8. Group 2: Medscand CytoBrush Plus GT (n = 7. Group 3: Rover spatula + endocervix brush (n = 11. Group 4: Medscand spatula + CytoBrush Plus GT (n = 11. Results Examination of SurePath broom-collected cytology yielded the following abnormal diagnoses: atypia (n = 2, LSIL (n = 5 and HSIL (n = 3. Comparison of these diagnoses to those obtained from paired samples using the additional collection devices showed that use of a second and or third device yielded no additional abnormal diagnoses. Importantly, use of additional devices did not improve upon the abnormal cell recovery of the SurePath broom and in 4/10 cases under-predicted or did not detect the SurePath broom-collected lesion as confirmed by cervical biopsy. Finally, in 36/37 cases, the SurePath broom successfully recovered ECs. Use of additional devices, in Group 3, augmented EC recovery to 37/37. Conclusions Use of additional collection devices in conjunction with the SurePath broom did not enhance diagnostic utility of the SurePath Pap. A potential but not significant improvement in EC recovery might be seen with the use of three devices.

  6. Seedling germination success and survival of the invasive shrub Scotch broom ( Cytisus scoparius) in response to fire and experimental clipping in the montane grasslands of the Nilgiris, south India

    Science.gov (United States)

    Srinivasan, Madhusudan P.; Kalita, Ratul; Gurung, Inder K.; Bhattacharjee, Saroj K.; Antony, Predit M.; Krishnan, Suresh; Gleeson, Scott K.

    2012-01-01

    The spread of the exotic shrub Scotch broom in the montane grasslands of the Nilgiris is one of the major threats to biodiversity and ecosystem function there. It is likely that fire suppression over the past few decades is the proximate cause of expansion of broom populations. This study capitalizes on a wildfire event to examine fire effects on mature broom populations and soil seedbanks. Fire resulted in widespread death of mature broom stands but also stimulated broom soil seedbanks. However, this initial difference in seedling densities in burned and unburned plots was lost over time due to continuous recruitment in unburned plots. In a seed addition experiment, plots which were clipped prior to the fire showed higher germination success, possibly because fire temperature was moderated by biomass removal. In another experiment, non-dormant broom seeds were added to burned plots, which then received clipping treatments; there were no differences in broom seedling survival in clipped vs. unclipped plots. Overall, these results suggest that prescribed burning might contribute to the control of Scotch broom invasion by helping eliminate mature stands without significantly increasing regeneration from seed.

  7. Apoplastic and intracellular plant sugars regulate developmental transitions in witches’ broom disease of cacao

    Science.gov (United States)

    Barau, Joan; Grandis, Adriana; Carvalho, Vinicius Miessler de Andrade; Teixeira, Gleidson Silva; Zaparoli, Gustavo Henrique Alcalá; do Rio, Maria Carolina Scatolin; Rincones, Johana; Buckeridge, Marcos Silveira; Pereira, Gonçalo Amarante Guimarães

    2015-01-01

    Witches’ broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant–fungus interface, the apoplastic fluid of cacao. Data are provided supporting a role for the dynamics of soluble carbon in the apoplastic fluid in prompting the end of the biotrophic phase of infection. Carbon depletion and the consequent fungal sensing of starvation were identified as key signalling factors at the apoplast. MpNEP2, a fungal effector of host necrosis, was found to be up-regulated in an autophagic-like response to carbon starvation in vitro. In addition, the in vivo artificial manipulation of carbon availability in the apoplastic fluid considerably modulated both its expression and plant necrosis rate. Strikingly, infected cacao tissues accumulated intracellular hexoses, and showed stunted photosynthesis and the up-regulation of senescence markers immediately prior to the transition to the necrotrophic phase. These opposite findings of carbon depletion and accumulation in different host cell compartments are discussed within the frame of WBD development. A model is suggested to explain phase transition as a synergic outcome of fungal-related factors released upon sensing of extracellular carbon starvation, and an early senescence of infected tissues probably triggered by intracellular sugar accumulation. PMID:25540440

  8. Apoplastic and intracellular plant sugars regulate developmental transitions in witches' broom disease of cacao.

    Science.gov (United States)

    Barau, Joan; Grandis, Adriana; Carvalho, Vinicius Miessler de Andrade; Teixeira, Gleidson Silva; Zaparoli, Gustavo Henrique Alcalá; do Rio, Maria Carolina Scatolin; Rincones, Johana; Buckeridge, Marcos Silveira; Pereira, Gonçalo Amarante Guimarães

    2015-03-01

    Witches' broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant-fungus interface, the apoplastic fluid of cacao. Data are provided supporting a role for the dynamics of soluble carbon in the apoplastic fluid in prompting the end of the biotrophic phase of infection. Carbon depletion and the consequent fungal sensing of starvation were identified as key signalling factors at the apoplast. MpNEP2, a fungal effector of host necrosis, was found to be up-regulated in an autophagic-like response to carbon starvation in vitro. In addition, the in vivo artificial manipulation of carbon availability in the apoplastic fluid considerably modulated both its expression and plant necrosis rate. Strikingly, infected cacao tissues accumulated intracellular hexoses, and showed stunted photosynthesis and the up-regulation of senescence markers immediately prior to the transition to the necrotrophic phase. These opposite findings of carbon depletion and accumulation in different host cell compartments are discussed within the frame of WBD development. A model is suggested to explain phase transition as a synergic outcome of fungal-related factors released upon sensing of extracellular carbon starvation, and an early senescence of infected tissues probably triggered by intracellular sugar accumulation.

  9. Transcriptomic analysis of Paulownia infected by Paulownia witches'-broom Phytoplasma.

    Directory of Open Access Journals (Sweden)

    Hai-Qing Mou

    Full Text Available Phytoplasmas are plant pathogenic bacteria that have no cell wall and are responsible for major crop losses throughout the world. Phytoplasma-infected plants show a variety of symptoms and the mechanisms they use to physiologically alter the host plants are of considerable interest, but poorly understood. In this study we undertook a detailed analysis of Paulownia infected by Paulownia witches'-broom (PaWB Phytoplasma using high-throughput mRNA sequencing (RNA-Seq and digital gene expression (DGE. RNA-Seq analysis identified 74,831 unigenes, which were subsequently used as reference sequences for DGE analysis of diseased and healthy Paulownia in field grown and tissue cultured plants. Our study revealed that dramatic changes occurred in the gene expression profile of Paulownia after PaWB Phytoplasma infection. Genes encoding key enzymes in cytokinin biosynthesis, such as isopentenyl diphosphate isomerase and isopentenyltransferase, were significantly induced in the infected Paulownia. Genes involved in cell wall biosynthesis and degradation were largely up-regulated and genes related to photosynthesis were down-regulated after PaWB Phytoplasma infection. Our systematic analysis provides comprehensive transcriptomic data about plants infected by Phytoplasma. This information will help further our understanding of the detailed interaction mechanisms between plants and Phytoplasma.

  10. Transcriptomic analysis of Paulownia infected by Paulownia witches'-broom Phytoplasma.

    Science.gov (United States)

    Mou, Hai-Qing; Lu, Jie; Zhu, Shui-Fang; Lin, Cai-Li; Tian, Guo-Zhong; Xu, Xia; Zhao, Wen-Jun

    2013-01-01

    Phytoplasmas are plant pathogenic bacteria that have no cell wall and are responsible for major crop losses throughout the world. Phytoplasma-infected plants show a variety of symptoms and the mechanisms they use to physiologically alter the host plants are of considerable interest, but poorly understood. In this study we undertook a detailed analysis of Paulownia infected by Paulownia witches'-broom (PaWB) Phytoplasma using high-throughput mRNA sequencing (RNA-Seq) and digital gene expression (DGE). RNA-Seq analysis identified 74,831 unigenes, which were subsequently used as reference sequences for DGE analysis of diseased and healthy Paulownia in field grown and tissue cultured plants. Our study revealed that dramatic changes occurred in the gene expression profile of Paulownia after PaWB Phytoplasma infection. Genes encoding key enzymes in cytokinin biosynthesis, such as isopentenyl diphosphate isomerase and isopentenyltransferase, were significantly induced in the infected Paulownia. Genes involved in cell wall biosynthesis and degradation were largely up-regulated and genes related to photosynthesis were down-regulated after PaWB Phytoplasma infection. Our systematic analysis provides comprehensive transcriptomic data about plants infected by Phytoplasma. This information will help further our understanding of the detailed interaction mechanisms between plants and Phytoplasma.

  11. Identification of genes related to Paulownia witches' broom by AFLP and MSAP.

    Science.gov (United States)

    Cao, Xibing; Fan, Guoqiang; Deng, Minjie; Zhao, Zhenli; Dong, Yanpeng

    2014-08-21

    DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches' broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L(-1) MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.

  12. Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP

    Directory of Open Access Journals (Sweden)

    Xibing Cao

    2014-08-01

    Full Text Available DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS using amplified fragment length polymorphism (AFLP and methylation-sensitive amplification polymorphism (MSAP techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.

  13. A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao

    Directory of Open Access Journals (Sweden)

    Bailey Bryan A

    2008-11-01

    Full Text Available Abstract Background The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD in cacao (Theobroma cacao. It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9× coverage of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. Results Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin. Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. Conclusion This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa

  14. Design of the driving system for visible near-infrared spatial programmable push-broom remote CCD sensor

    Science.gov (United States)

    Xu, Zhipeng; Wei, Jun; Zhou, Qianting; Weng, Dongshan; Li, Jianwei

    2010-11-01

    VNIR multi-spectral image sensor has wide applications in remote sensing and imaging spectroscopy. An image spectrometer of a spatial remote programmable push-broom sensing satellite requires visible near infrared band ranges from 0.4μm to 1.04μm which is one of the most important bands in remote sensing. This paper introduces a method of design the driving system for 1024x1024 VNIR CCD sensor for programmable push-broom remote sensing. The digital driving signal is generated by the FPGA device. There are seven modules in the FPGA program and all the modules are coded by VHDL. The driving system have five mainly functions: drive the sensor as the demand of timing schedule, control the AD convert device to work, get the parameter via RS232 from control platform, process the data input from the AD device, output the processed data to PCI sample card to display in computer end. All the modules above succeed working on FPGA device APA600. This paper also introduced several important keys when designing the driving system including module synchronization, critical path optimization.

  15. Molecular identification of the phytoplasma associated with kerria witches'-broom%棣棠丛枝病相关植原体的分子鉴定

    Institute of Scientific and Technical Information of China (English)

    常文程; 李向东; 邵云华; 徐加利; 竺晓平

    2012-01-01

    By using universal primers for phytoplasmal 16S rRNA and tuf genes, two specific fragments of 1 243 bp and 842 bp in length were amplified with nested-PCR from the DNA of kerria showing witches' -broom symptom. The fragments were sequenced and subjected to identity and phylogenetic analysis. The results indicated that the 16S rRNA and tuf gene of phytoplasma isolate from kerria witches' -broom (KWB) shared the highest identities of 99. 4% and 99. 5% , respectively, with paulownia witches' -broom phytoplasma (PaWB) belonged to subgroup D of aster yellows group (16SrI-D). In the phylogenetic trees constructed with 16S rRNA and tuf genes, KWB was clustered with phytoplasmas of 16SrI-D. The 16S rRNA gene of KWB had identical patterns with a 16SrI-D phytoplasma for Alu I , Hha I , Mse I and Taq I in the virtual RFLP analysis. All these results indicated that the phytoplsama associated with kerria witches' -broom was belonged to the subgroup D of aster yellows group (16SrI-D).

  16. Multilocus sequences confirm the close genetic relationship of four phytoplasmas of peanut witches'-broom group 16SrII-A.

    Science.gov (United States)

    Li, Yong; Piao, Chun-gen; Tian, Guo-zhong; Liu, Zhi-xin; Guo, Min-wei; Lin, Cai-li; Wang, Xi-zhuo

    2014-08-01

    Four witches'-broom diseases associated with Arachis hypogaea (peanut), Crotalaria pallida, Tephrosia purpurea, and Cleome viscosa were observed in Hainan Province, China during field surveys in 2004, 2005, and 2007. In previously reported studies, we identified these four phytoplasmas as members of subgroup 16SrII-A, and discovered that their 16S rRNA gene sequences were 99.9-100% identical to one another. In this study, we performed extensive phylogenetic analyses to elucidate relationships among them. We analyzed sequences of the 16S rRNA gene and rplV-rpsC, rpoB, gyrB, dnaK, dnaJ, recA, and secY combined sequence data from two strains each of the four phytoplasmas from Hainan province, as well as strains of peanut witches'-broom from Taiwan (PnWB-TW), "Candidatus Phytoplasma australiense", "Ca. Phytoplasma mali AT", aster yellows witches'-broom phytoplasma AYWB, and onion yellows phytoplasma OY-M. In the 16S rRNA phylogenetic tree, the eight Hainan strains form a clade with PnWB-TW. Analysis of the seven concatenated gene regions indicated that the four phytoplasmas collected from Hainan province cluster most closely with one another, but are closely related to PnWB-TW. The results of field survey and phylogenetic analysis indicated that Cr. pallida, T. purpurea, and Cl. viscosa may be natural plant hosts of peanut witches'-broom phytoplasma.

  17. Development of biomarkers and a diagnostic tool for investigation of coinfections by and interactions between potato purple top and potato witches’-broom phytoplasmas in tomato

    Science.gov (United States)

    Columbia Basin potato purple top (PPT) phytoplasma and Alaska potato witches’-broom (PWB) phytoplasma are two closely-related but mutually distinct pathogenic bacteria that infect potato and other vegetable crops. Inhabiting phloem sieve elements and being transmitted by phloem-feeding insect vecto...

  18. Working with grocers to reduce dietary sodium: lessons learned from the Broome County Sodium Reduction in Communities pilot project.

    Science.gov (United States)

    Johnston, Yvonne A; McFadden, Mary; Lamphere, Marissa; Buch, Karen; Stark, Beth; Salton, Judith Lynn

    2014-01-01

    The purpose of this article is to describe implementation of and lessons learned from the Broome County Sodium Reduction in Communities grocery store initiative. This pilot project was conducted in collaboration with a regional supermarket chain and endeavored to develop population-based strategies for reducing sodium intake. Key interventions included marketing strategies, taste test demonstrations, and a public media campaign. Project staff worked closely with corporate registered dietitian nutritionists, a nutrition specialist, and an advertising agency in its development and implementation. A social marketing approach was used to educate consumers about the hidden sources of dietary sodium, to raise awareness of the adverse health effects of excess sodium intake, to encourage consumers to read food labels, and to urge them to purchase food items lower in sodium. The lessons learned from this experience may be of assistance to other communities that seek to implement similar sodium-reduction strategies in the grocery store environment.

  19. Push-Broom-Type Very High-Resolution Satellite Sensor Data Correction Using Combined Wavelet-Fourier and Multiscale Non-Local Means Filtering.

    Science.gov (United States)

    Kang, Wonseok; Yu, Soohwan; Seo, Doochun; Jeong, Jaeheon; Paik, Joonki

    2015-09-10

    In very high-resolution (VHR) push-broom-type satellite sensor data, both destriping and denoising methods have become chronic problems and attracted major research advances in the remote sensing fields. Since the estimation of the original image from a noisy input is an ill-posed problem, a simple noise removal algorithm cannot preserve the radiometric integrity of satellite data. To solve these problems, we present a novel method to correct VHR data acquired by a push-broom-type sensor by combining wavelet-Fourier and multiscale non-local means (NLM) filters. After the wavelet-Fourier filter separates the stripe noise from the mixed noise in the wavelet low- and selected high-frequency sub-bands, random noise is removed using the multiscale NLM filter in both low- and high-frequency sub-bands without loss of image detail. The performance of the proposed method is compared to various existing methods on a set of push-broom-type sensor data acquired by Korean Multi-Purpose Satellite 3 (KOMPSAT-3) with severe stripe and random noise, and the results of the proposed method show significantly improved enhancement results over existing state-of-the-art methods in terms of both qualitative and quantitative assessments.

  20. Experimental study on the push-broom infrared imaging system based on line-plane-switching fiber bundle

    Science.gov (United States)

    Yan, Xingtao; Li, Fu; Ma, Xiaolong; Lv, Juan; He, Yinghong; Zhao, Yiyi; Bu, Fan

    2016-10-01

    The use of line-plane-switching infrared fiber bundle to achieve wide field of view push-broom infrared imaging has been studied with experiment. In this technology, the linear array end of the imaging fiber bundle is used as a long-linear array infrared detector, and the plane array end of the bundle is coupled by a mature small scale Infrared Focal Plane Array (IRFPA). It can evade the difficulty of getting the long-linear array infrared detector directly, and has a signally significance to the development of internal infrared imaging technology. Based on the introduction of the composition, working principle of this novel infrared optical system, the system principle-demonstrating experiment has been accomplished. The line-plane-switching fiber bundle used in this experiment is 64×9 format plane array and 192×3 format linear array. It is made from chalcogenide glass fibers, possessing core (As40S59.5Se0.5) of 45 μm, cladding (As40S60) of 5 μm, and error of 1% in diameter. Perfect imaging results prove that this novel technology is feasibility and superiority. The analysis of the experiment makes a foundation for the subsequent further verification experiments.

  1. Morphological changes of Paulownia seedlings infected phytoplasmas reveal the genes associated with witches' broom through AFLP and MSAP.

    Science.gov (United States)

    Cao, Xibing; Fan, Guoqiang; Zhao, Zhenli; Deng, Minjie; Dong, Yanpeng

    2014-01-01

    Paulownia witches' broom (PaWB) caused by phytoplasma might result in devastating damage to the growth and wood production of Paulownia. To study the effect of phytoplasma on DNA sequence and to discover the genes related to PaWB occurrence, DNA polymorphisms and DNA methylation levels and patterns in PaWB seedlings, the ones treated with various concentration of methyl methane sulfonate (MMS) and healthy seedlings were investigated with amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP). Our results indicated that PaWB seedlings recovered a normal morphology, similar to healthy seedlings, after treatment with more than 20 mg · L-1 MMS; Phytoplasma infection did not change the Paulownia genomic DNA sequence at AFLP level, but changed the global DNA methylation levels and patterns; Genes related to PaWB were discovered through MSAP and validated using quantitative real-time PCR (qRT-PCR). These results implied that changes of DNA methylation levels and patterns were closely related to the morphological changes of seedlings infected with phytoplasmas.

  2. Discovery of genes related to witches broom disease in Paulownia tomentosa × Paulownia fortunei by a De Novo assembled transcriptome.

    Science.gov (United States)

    Liu, Rongning; Dong, Yanpeng; Fan, Guoqiang; Zhao, Zhenli; Deng, Minjie; Cao, Xibing; Niu, Suyan

    2013-01-01

    In spite of its economic importance, very little molecular genetics and genomic research has been targeted at the family Paulownia spp. The little genetic information on this plant is a big obstacle to studying the mechanisms of its ability to resist Paulownia Witches' Broom (PaWB) disease. Analysis of the Paulownia transcriptome and its expression profile data are essential to extending the genetic resources on this species, thus will greatly improves our studies on Paulownia. In the current study, we performed the de novo assembly of a transcriptome on P. tomentosa × P. fortunei using the short-read sequencing technology (Illumina). 203,664 unigenes with a mean length of 1,328 bp was obtained. Of these unigenes, 32,976 (30% of all unigenes) containing complete structures were chosen. Eukaryotic clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed of these unigenes. Genes related to PaWB disease resistance were analyzed in detail. To our knowledge, this is the first study to elucidate the genetic makeup of Paulownia. This transcriptome provides a quick way to understanding Paulownia, increases the number of gene sequences available for further functional genomics studies and provides clues to the identification of potential PaWB disease resistance genes. This study has provided a comprehensive insight into gene expression profiles at different states, which facilitates the study of each gene's roles in the developmental process and in PaWB disease resistance.

  3. Discovery of genes related to witches broom disease in Paulownia tomentosa × Paulownia fortunei by a De Novo assembled transcriptome.

    Directory of Open Access Journals (Sweden)

    Rongning Liu

    Full Text Available In spite of its economic importance, very little molecular genetics and genomic research has been targeted at the family Paulownia spp. The little genetic information on this plant is a big obstacle to studying the mechanisms of its ability to resist Paulownia Witches' Broom (PaWB disease. Analysis of the Paulownia transcriptome and its expression profile data are essential to extending the genetic resources on this species, thus will greatly improves our studies on Paulownia. In the current study, we performed the de novo assembly of a transcriptome on P. tomentosa × P. fortunei using the short-read sequencing technology (Illumina. 203,664 unigenes with a mean length of 1,328 bp was obtained. Of these unigenes, 32,976 (30% of all unigenes containing complete structures were chosen. Eukaryotic clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed of these unigenes. Genes related to PaWB disease resistance were analyzed in detail. To our knowledge, this is the first study to elucidate the genetic makeup of Paulownia. This transcriptome provides a quick way to understanding Paulownia, increases the number of gene sequences available for further functional genomics studies and provides clues to the identification of potential PaWB disease resistance genes. This study has provided a comprehensive insight into gene expression profiles at different states, which facilitates the study of each gene's roles in the developmental process and in PaWB disease resistance.

  4. Population genetic analysis reveals a low level of genetic diversity of 'Candidatus Phytoplasma aurantifolia' causing witches' broom disease in lime.

    Science.gov (United States)

    Al-Abadi, Shaikha Y; Al-Sadi, Abdullah M; Dickinson, Matthew; Al-Hammadi, Mohammed S; Al-Shariqi, Rashid; Al-Yahyai, Rashid A; Kazerooni, Elham A; Bertaccini, Assunta

    2016-01-01

    Witches' broom disease of lime (WBDL) is a serious phytoplasma disease of acid lime in Oman, the UAE and Iran. Despite efforts to study it, no systemic study attempted to characterize the relationship among the associated phytoplasma, 'Candidatus Phytoplasma aurantifolia', from the three countries. This study utilized sequences of the 16S rRNA, imp and secA genes to characterize 57 strains collected from Oman (38), the UAE (9) and Iran (10). Phylogenetic analysis based on the 16S rRNA gene showed that the 57 strains shared 98.5-100 % nucleotide similarity to each other and to strains of 'Ca. P. aurantifolia' available in GenBank. The level of genetic diversity was low based on the 16S rRNA (0-0.011), imp (0-0.002) and secA genes (0-0.015). The presence of low level of diversity among phytoplasma strains from Oman, the UAE and Iran can be explained by the movement of infected lime seedlings from one country to another through trading and exchange of infected plants. The study discusses implication of the findings on WBDL spread and management.

  5. Morphological changes of Paulownia seedlings infected phytoplasmas reveal the genes associated with witches' broom through AFLP and MSAP.

    Directory of Open Access Journals (Sweden)

    Xibing Cao

    Full Text Available Paulownia witches' broom (PaWB caused by phytoplasma might result in devastating damage to the growth and wood production of Paulownia. To study the effect of phytoplasma on DNA sequence and to discover the genes related to PaWB occurrence, DNA polymorphisms and DNA methylation levels and patterns in PaWB seedlings, the ones treated with various concentration of methyl methane sulfonate (MMS and healthy seedlings were investigated with amplified fragment length polymorphism (AFLP and methylation-sensitive amplification polymorphism (MSAP. Our results indicated that PaWB seedlings recovered a normal morphology, similar to healthy seedlings, after treatment with more than 20 mg · L-1 MMS; Phytoplasma infection did not change the Paulownia genomic DNA sequence at AFLP level, but changed the global DNA methylation levels and patterns; Genes related to PaWB were discovered through MSAP and validated using quantitative real-time PCR (qRT-PCR. These results implied that changes of DNA methylation levels and patterns were closely related to the morphological changes of seedlings infected with phytoplasmas.

  6. 小孢盘菌(原变型)的生物学特性%Study on the biological characters of Acervus epispartius (Berk.& Broome) Pfister forma epispartius

    Institute of Scientific and Technical Information of China (English)

    钟连顺; 翁垂芳; 肖胜刚; 刘叶高; 林汝楷

    2009-01-01

    小孢盘菌(原变型)[Acervus epispartius (Berk.& Broome) Pfister forma epispartius]菌丝在PDA和加富培养基上生长良好.适宜温度为25-32 ℃,适宜含水量为60%-70%.中性和弱碱性环境有利于菌丝生长,但最适pH值为8.菌丝生长对光照度要求不严,但光照度为500 lx时菌丝生长最佳.

  7. Germination from Different Organs of Witches'-broom Paulownia%患丛枝病泡桐不同器官芽诱导研究

    Institute of Scientific and Technical Information of China (English)

    陈丽文; 王辉

    2014-01-01

    以患病豫杂-1号泡桐的叶片、叶柄、茎段为材料,通过在培养基中施用不同的激素,观察材料的长芽情况,筛选出最佳外植体及最佳激素组合。实验结果表明:患病豫杂-1号泡桐芽诱导的最佳外植体是茎段,相应的培养基及激素组合为MS培养基,NAA0.3mg/L,6-BA12mg/L。此方案下的芽诱导率为30.19%。%Using leaves, leaf stalks and stems of wiches'-broom paulownia as materials , applying different hormones in culture medi-um, observing the germination of materials , and obtaining the best exophyte and hormones combination .The results are as following:The best exophyte of germination of wiches'-broom Yuza-1 paulownia is stem , the best culture medium and hormone combination is MS and NAA0.3mg/L and 6-BA12mg/L, the inductivity of shoot is 30.19%.

  8. Diversity of endophytic fungal community of cacao (Theobroma cacao L. and biological control of Crinipellis perniciosa, causal agent of Witches' Broom Disease

    Directory of Open Access Journals (Sweden)

    2005-02-01

    Full Text Available The basidiomycete fungus Crinipellis perniciosa (Stahel Singer is the causal agent of Witches' Broom Disease of Cacao (Theobroma cacao L. which is the main factor limiting cacao production in the Americas. Pod losses of up to 90% are experienced in affected areas as evidenced by the 50% drop in production in Bahia province, Brazil following the arrival of the C. perniciosa in the area in 1989. The disease has proven particularly difficult to control and many farmers in affected areas have given up cacao cultivation. In order to evaluate the potential of endophytes as a biological control agent of this phytopathogen, the endophytic fungal community of resistant and susceptible cacao plants as well as affected branches was studied between 2001 and 2002. The fungal community was identified by morphological traits and rDNA sequencing as belonging to the genera Acremonium, Blastomyces, Botryosphaeria, Cladosporium, Colletotrichum, Cordyceps, Diaporthe, Fusarium, Geotrichum, Gibberella, Gliocladium, Lasiodiplodia, Monilochoetes, Nectria, Pestalotiopsis, Phomopsis, Pleurotus, Pseudofusarium, Rhizopycnis, Syncephalastrum, Trichoderma, Verticillium and Xylaria. These fungi were evaluated both in vitro and in vivo by their ability to inhibit C. perniciosa. Among these, some were identified as potential antagonists, but only one fungus (Gliocladium catenulatum reduced the incidence of Witches' Broom Disease in cacao seedlings to 70%.

  9. Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa

    Directory of Open Access Journals (Sweden)

    Marco Janice Lisboa De

    2003-01-01

    Full Text Available Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, beta-1,3-glucanase, total cellulase, endoglucanase, aryl- beta-glucosidase, beta-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence of specific substrates. Except for N-acetylglucosaminidase and beta-glucosidase Trichoderma harzianum isolate 1051 produced the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between Trichoderma and C. perniciosa is discussed.

  10. Superfund record of decision (EPA Region 2): Endicott Village Well Field, Broome County, NY. (Third remedial action), September 1992. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1992-09-30

    The 16-acre Endicott Village Well Field site is an inactive landfill in the Village of Endicott, Broome County, New York. The site includes a municipal drinking water supply well, known as the Ranney well. In May 1981, EPA detected vinyl chloride and other VOCs in the Ranney well discharge. The ROD addresses the Endicott Village landfill no. 1, the source of the site contamination, as OU2. The primary contaminants of concern affecting the soil, debris, and ground water are VOCs including 1,2-DCE, benzene, PCE, TCE, toluene, vinyl chloride, and xylenes; other organics including PAHs, PCBs, and pesticides; and metals, including lead. The selected remedial action for this site includes capping the majority of landfill no. 1 with a low permeability soil cap; covering the Tri-Cities Airport Controlled Activity Area and the compost facility area with a bituminous (asphalt) cap.

  11. BROOME COUNTY, NEW YORK

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  12. Functional and biophysical studies on four ceratoplatanins from the fungus Moniliophthora perniciosa, causal agent of the Witche's broom disease

    Energy Technology Data Exchange (ETDEWEB)

    Barsottini, M.; Zaparoli, G.; Garcia, O.; Pereira, G.A.G. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil); Oliveira, J.F.; Tiezzi, H.O.; Ambrosio, A.L.B.; Dias, S.M.G. [Laboratorio Nacional de Biociencias - LNBIO, Campinas, SP (Brazil)

    2012-07-01

    Full text: Ceratoplatanin (CP) is a secreted protein of 12.4 kDa initially identified in culture filtrates of the disease ascomycete Ceratocystis fimbriata f. sp. platani, etiological agent of the canker stain disease. CP is also the founding member of the namesake protein family, which contains fungal-secreted proteins involved in various stages of the host-fungus interaction and may act as phytotoxins or elicitors of defense response. Besides the low molecular weight, CPs have a high percentage of hydrophobic residues and share two conserved intramolecular disulfide bonds. It has been suggested that CPs have important physiological functions, including interaction with cell wall or cell membrane and manipulation of the host's defense system. Furthermore, a recent work showed that the ceratoplatanin from C. fimbriata has some degree of affinity for the saccharide 4-N-acetylglucosamine. However, its precise molecular function remains elusive. Five putative CPs have been identified in Moniliophthora perniciosa a basidiomycete fungus responsible for great economic losses in cocoa industry in the form of Witches' broom disease (WBD) , four of which had their crystal structures resolved by our group. In this work we report biophysical and functional studies on these MpCPs aiming at understanding their role and importance during the WBD progression. (author)

  13. A multidisciplinary approach to digital mapping of dinosaurian tracksites in the Lower Cretaceous (Valanginian–Barremian) Broome Sandstone of the Dampier Peninsula, Western Australia

    Science.gov (United States)

    Hacker, Jorg M.; Zlot, Robert; Poropat, George; Bosse, Michael; Salisbury, Steven W.

    2017-01-01

    The abundant dinosaurian tracksites of the Lower Cretaceous (Valanginian–Barremian) Broome Sandstone of the Dampier Peninsula, Western Australia, form an important part of the West Kimberley National Heritage Place. Previous attempts to document these tracksites using traditional mapping techniques (e.g., surface overlays, transects and gridlines combined with conventional photography) have been hindered by the non-trivial challenges associated with working in this area, including, but not limited to: (1) the remoteness of many of the tracksites; (2) the occurrence of the majority of the tracksites in the intertidal zone; (3) the size and complexity of many of the tracksites, with some extending over several square kilometres. Using the historically significant and well-known dinosaurian tracksites at Minyirr (Gantheaume Point), we show how these issues can be overcome through the use of an integrated array of remote sensing tools. A combination of high-resolution aerial photography with both manned and unmanned aircraft, airborne and handheld high-resolution lidar imaging and handheld photography enabled the collection of large amounts of digital data from which 3D models of the tracksites at varying resolutions were constructed. The acquired data encompasses a very broad scale, from the sub-millimetre level that details individual tracks, to the multiple-kilometre level, which encompasses discontinuous tracksite exposures and large swathes of coastline. The former are useful for detailed ichnological work, while the latter are being employed to better understand the stratigraphic and temporal relationship between tracksites in a broader geological and palaeoecological context. These approaches and the data they can generate now provide a means through which digital conservation and temporal monitoring of the Dampier Peninsula’s dinosaurian tracksites can occur. As plans for the on-going management of the tracks in this area progress, analysis of the 3D data

  14. Discovery of microRNAs and transcript targets related to witches' broom disease in Paulownia fortunei by high-throughput sequencing and degradome approach.

    Science.gov (United States)

    Niu, Suyan; Fan, Guoqiang; Deng, Minjie; Zhao, Zhenli; Xu, Enkai; Cao, Lin

    2016-02-01

    Paulownia witches' broom (PaWB) caused by the phytoplasma is a devastating disease of Paulownia trees. It has caused heavy yield losses to Paulownia production worldwide. However, knowledge of the transcriptional and post-transcriptional regulation of gene expression by microRNAs (miRNAs), especially miRNAs responsive to PaWB disease stress, is still rudimentary. In this study, to identify miRNAs and their transcript targets that are responsive to PaWB disease stress, six sequencing libraries were constructed from healthy (PF), PaWB-infected (PFI), and PaWB-infected, 20 mg L(-1) methyl methane sulfonate-treated (PFI20) P. fortunei seedlings. As a result, 95 conserved miRNAs belonging to 18 miRNA families, as well as 122 potential novel miRNAs, were identified. Most of them were found to be a response to PaWB disease-induced stress, and the expression levels of these miRNAs were validated by quantitative real-time PCR analysis. The study simultaneously identified 109 target genes from the P. fortunei for 14 conserved miRNA families and 24 novel miRNAs by degradome sequencing. Furthermore, the functions of the miRNA targets were annotated based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The results presented here provide the groundwork for further analysis of miRNAs and target genes responsive to the PaWB disease stress, and could be also useful for addressing new questions to better understand the mechanisms of plant infection by phytoplasma in the future.

  15. Marcadores microssatélites relacionados com a resistência à vassoura-de-bruxa do cacaueiro Microsatellite markers related to resistance of cocoa tree against witches'-broom

    Directory of Open Access Journals (Sweden)

    Rogério Mercês Ferreira Santos

    2007-08-01

    Full Text Available Os objetivos deste trabalho foram caracterizar a resistência à vassoura-de-bruxa de plantas de cacau originadas do cruzamento entre TSH 1188 e CCN 51 (população segregante, por meio de dois métodos de inoculação em condições de campo, e identificar marcadores microssatélites específicos para grupos de plantas resistentes e suscetíveis. As plantas-controle avaliadas pelos métodos de inoculação natural e inoculação artificial em campo produziram os mesmos padrões de sintomas. As plantas da população segregante também coincidiram os padrões de sintomas em 90%, por esses dois métodos. O método de inoculação artificial em campo permite detectar falso-resistentes. Dos 18 pares de primers microssatélites amplificados, 15 foram polimórficos entre os genitores, e seis entre os grupos de plantas segregantes contrastantes quanto à resistência à vassoura-de-bruxa. Foram confirmadas três marcas previamente associadas a QTL (locos para características quantitativas relacionados com a resistência à vassoura-de-bruxa, comuns a outras populações. Também foram identificados três novos QTL para esta característica, típicos desta população, o que comprova sua utilidade para o melhoramento genético do cacaueiro.The objectives of this work were to evaluate cocoa tree resistance against witches'-broom, in plants originated from the crossing between TSH 1188 and CCN 51 (segregating population, by means of two methods of inoculation in field conditions, and to identify microsatellite markers specific for resistant and susceptible plants. The control plants bore identical symptoms as the plants of the segregating population in 90% of the cases under the two methods. The method of artificial inoculation in the field allows the detection of false resistance to the disease. Of the 18 pairs of microsatellite primers amplified, 15 were polymorphic between genitors and six were polymorphic between the two groups of plants evaluated

  16. Witches' brooms in Siberian stone pine as somatic mutations and initial genetic material for breeding of nut-bearing and ornamental cultivars

    Directory of Open Access Journals (Sweden)

    M. S. Yamburov

    2013-12-01

    Full Text Available For the raising of the Siberian stone pine (Pinus sibirica Du Tour nut-bearing and ornamental cultivars, the most important traits are adense crown, slow growth and precocity. Generative mutations of this kind are eliminated by natural selection, therefore, somatic mutationrsearch is important. Among somatic mutations, the most promising one is the so-called 'witches' brooms' (WB where crown fragments demonstrate slowed growth and intensive branching. WB occasionally occurs in native populations. According to phytopathology textbooks, WB are caused by various pathogen species (viruses, mycoplasmas, fungi. The WB of this kind are characterized by a sickly look, full suppression of reproductive functions, a short life and a nidus patternof distribution. There are also WB of different types: with a high vitality and fertility, a long life and sporadic distribution. They occur very rarely(about 1 per 10 000 trees across the species' range. We investigated 18 trees with WB of this type. The size of WB ranged from 0.3 to 30 m, age varied from 30 to 300 years. Male cones were absent inall WB. Female cone initiation was normal if the WB was located in the top part of a crown. Scions from WB and a normal crown (NC of the same tree were grafted on identical rootstocks. On average,the height of 7-year-old WB grafts (WBG was 2 times lower, and the stem diameter was 2 times higher than in the NC grafts (NCG. It wasachieved due to the fundamental differences in the shoot system morphogenesis. Here are three principal differences in decreasing order of importance: (i WBGs were characterized by the absence or near absence of apical dominance. The NCG had no more than 3 orders of branching, and the length of the 1st order axis was on average 5times larger than the axis of the 3rd order. The WBG had 6-7 orders of branching, and the length of shoots of 5-6th orders averaged 80-90% of the length of the first orders. (ii At an

  17. 甲基磺酸甲酯处理毛泡桐丛枝病幼苗的形态变化及SSR分析%Morphological changes of the witches' broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis

    Institute of Scientific and Technical Information of China (English)

    曹喜兵; 范国强; 翟晓巧

    2012-01-01

    Witches' broom is one of the most serious diseases in Paulownia production. Morphological changes of the witches' broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis were investigated in order to further study the relation' between the disease and DNA changes. The results indicated that the diseased seedlings treated with optimal concentration of MMS might turn into the ones without symptom morphologically, but there still existed the phytoplasma in the seedlings treated with 20 mg · L-1 MMS. Not only might MMS of which the concentrations were more than 80 mg · L-1 eliminates the phytoplasma from the seedlings, but also inhibits the seedling growth. DNA base sequences of the healthy, diseased and the diseased-treated with 20 and 100 mg · L- MMS seedlings were the same at SSR level.

  18. Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data Avaliação da diversidade genética em uma amostra de acessos de cacau resistentes à doença vassoura-de-bruxa, com base em dados de RAPD e pedigree

    OpenAIRE

    Ronaldo Carvalho dos Santos; José Luís Pires; Uilson Vanderley Lopes; Karina Peres G. Gramacho; Acassi Batista Flores; Rita de Cássia S. Bahia; Helaine C. Cristine Ramos; Ronan Xavier Corrêa; Dario Ahnert

    2005-01-01

    Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, ...

  19. 推扫式线阵TDI CCD扫描调制传递函数分析%Scanning modulation transfer function analysis of push-broom linear TDI CCD

    Institute of Scientific and Technical Information of China (English)

    杨永明; 李昕阳; 赵嘉鑫

    2011-01-01

    根据线阵TDI CCD离散采样的特点,以采样间距内成像调制度均值为基础,构建了推扫成像模式下线阵TDI CCD扫描方向的调制传递函数.该调制传递函数的数值分析表明:对于像元为10μm的线阵TDI CCD,行转移驱动时钟相数为4,3或2时,Nyquist频率处调制传递函数值分别为0.363,0.333或0.255;行频误差为1%及3%时,不同积分级数下调制传递函数变化曲线表明,增大行频误差及增加积分级数将使调制传递函数值减小,图像分辨率降低.成像实验结果符合所构建调制传递函数的定量分析结论.%Scanning modulation transfer function (MTF) for linear time-delay integration (TDD charge coupled device (CCD) using push-broom imaging mode was established. The MTF which took the discrete sampling characteristics of linear TDI CCD into account, was based on the imaging modulation mean within sampling interval. The numerical analysis of the proposed MTF indicated that the value of MTF at Nyquist frequency for linear TDI CCD with 10 fim pixel size was 0. 363, 0. 333 and 0. 255 respectively, when the number of row transfer phase clocks was 4, 3 and 2. When the row frequency error was 1% or 3%, the changes of MTF curves with different integration numbers implied that increasing the row frequency error and the integration number could decrease the value of MTF and reduce the image resolution. The results of imaging experiment agree with the quantity analysis of the proposed MTF.

  20. 泡桐丛枝植原体抗原膜蛋白基因序列分析与蛋白结构预测%Sequence Analysis and Structure Prediction of Antigenetic Membrane Protein Gene from Paulownia Witches'-Broom Phytoplasma

    Institute of Scientific and Technical Information of China (English)

    岳红妮; 吴云锋; 史英姿

    2009-01-01

    泡桐(Paulownia spp.)原产中国,栽培历史悠久。其自然分布或栽培区遍布全国23个省(市)、自治区,北起辽宁,南至广东、广西、云南南部,东起台湾及沿海诸省,西至甘肃。泡桐丛枝病又名泡桐扫帚病,由泡桐丛枝植原体(Paulownia witches’-broom phytoplasma,PaWB)侵染引起,严重地区发病率达80%以上,是泡桐生产上最重要的病害之一。

  1. New Broom To Sweep Clean At ABC

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The Agricultural Bank of China (ABC) has a new president. The last non- publicly traded state-owned lender in China announced on July 6 that Xiang Junbo, former Vice Governor of the People’s Bank of China, or the central bank, had beenappointed to take o

  2. Complete sequence of a full-length DNA and molecular characterization of one plasmid from chinaberry (Melia azedarach Z) witches'-broom phytoplasma%苦楝丛枝植原体质粒的测定与分子特征

    Institute of Scientific and Technical Information of China (English)

    宋传生; 林彩丽; 田国忠; 赵文军; 朱水芳; 牟海青; 胡佳续; 王曦茁; 郭民伟

    2011-01-01

    [ Objective ] To clone plasmid from chinaberry witches'-broom phytoplasma and analyse its molecular characterization. [Methods] Fragments of one plasmid (pCWBFq) in chinaberry witches'-broom phytoplasma-Fuqing strain ( CWBFq) were amplified with primer pairs which were designed according to plasmid sequences published on NCBI. Transmembrane domain and subcellular localization predictions of proteins encoded by the plasmid pCWBFq as well as phylogenetic analysis among the plasmid sequences were completed by using bioinformatic softwares. Southern blot analysis was performed to detect the plasmids existed in CWBFq and several other phytoplasmas with the pCWBFq repA probe. [ Results] One complete plasmid was sequenced from CWBFq. pCWBFq comprised 4446 bp and had a nucleotide content of 73. 5% A + T and encoded six proteins. Protein P2, P3, P4 and P5 of pCWBFq contained 3,2,1 and 2 tranmembrane domains respectively, and their predicted signal peptide values were 0.989, 0.505, 0.918 and 0.914 respectively. Homologous comparison showed that RepA homology between pCWBFq and other phytoplasmas was between 9. 6% -85. 6% , however, the homology of different SSB proteins was between 74. 0% - 89. 4% . Southern blotting withpCWBFq repA probe confirmed the existence of the plasmids in CWBFq. In addition, The hybridizations occurred with paulownia witches'-broom phytoplasma-Nanyang strain ( PaWBNy ) , periwinkle virescence phytoplasma-Hainan stanin (PeVHn) , chinaberry witches'-broom phytoplasma-Fuzhou strain ( CWBFz) and mulberry dwarf phytoplasma -Puyang strain (MDPy) , whereas, no hybridizarions occurred with jujube witches'-broom phytoplasma-Beijing strain ( JWBBj) , cherry lethal yellows phytoplasma-Xichang strain (CLYXc) and Bischofia polycarpa witches'-broom phytoplasma-Nanchang strain ( BiWBNc ). [ Conclusion ] The plasmid encoded a replication associated protein ( RepA) and a single-stranded DNA binding protein ( SSB) , which were for the replication of plasmid. Four

  3. Phenolic compounds, methylxanthines and antioxidant activity in cocoa mass and chocolates produced from "witch broom disease" resistant and non resistant cocoa cultivars Compostos fenólicos, metilxantinas e atividade antioxidante em massa de cacau e chocolates produzidos a partir de cultivares resistentes e não resistentes a "vassoura de bruxa"

    Directory of Open Access Journals (Sweden)

    Paula Bacelar Leite

    2013-06-01

    Full Text Available The "witch broom disease" caused by the fungus called Moniliophthora perniciosa is one of the most important cocoa diseases in Latin America, causing around 70% production reduction in the southern Bahia. In attempt to solve the problem, many cultivars resistant to the disease have been recommended to farmers. On the other hand, the chocolate flavour is composed by many compounds whose formation depends on the genetic background, environment where cocoa is grown and processing operations. Therefore, this work aimed at determining the monomeric phenolic compounds, methylxanthines and antioxidant activity of cocoa mass and dark chocolate from cocoa cultivars resistant to "witch broom disease" and non resistant to the disease. The total phenolic compounds in cocoa mass did not vary among cultivars with values ranging from 23.95mg g-1 to 25.03mg g-1. Chocolates made from non resistant cultivars showed higher total phenolic compounds (19.11mg g-1 than SR162 and PH16 with 16.08mg g-1 and 15.46mg g-1, respectively. Epicatechin had higher content than catechin and the levels of these two compounds were higher in SR162. There were significant differences among samples of cocoa mass analyzed for caffeine. Chocolate made from SR162 had the highest amount of monomeric compounds due to its high concentration of catechin and epicatechin. The chocolate sample with the highest antioxidant activity was the SR162, followed by non resistant blend and PH16, showing relationship between the antioxidant activity and monomeric phenolics content.A "vassoura bruxa" causada pelo fungo Moniliophthora perniciosa, é uma das doenças mais importantes do cacau na América Latina, provocando uma redução de cerca de 70% na produção das amêndoas na Bahia. Para tentar resolver o problema, muitos cultivares resistentes à enfermidade têm sido recomendados para os agricultores. Por outro lado, as características do chocolate são oriundas de várias substâncias, cuja forma

  4. Presence of a Phytoplasma Associated with Witches’-Broom Disease in Ugni molinae Turcz. and Gaultheria phillyreifolia (Pers. Sleumer Determined by DAPI, PCR, and DNA Sequencing Presencia de un Fitoplasma Asociado a la Enfermedad de "Escoba de Bruja" en Ugni molinae Turcz. y Gaultheria phillyreifolia (Pers. Sleumer Determinado Mediante DAPI, PCR y Secuenciación de ADN

    Directory of Open Access Journals (Sweden)

    Nolberto Arismendi S

    2010-03-01

    Full Text Available Murta (Ugni molinae Turcz. and common chaura (Gaultheria phillyreifolia (Pers. Sleumer are native species of Chile. Plants of both species have shown over-branching like witches' broom. The causal agents of these symptoms in many plants are phytoplasma. To verify the presence of these microorganisms, DAPI (4',6-diamidino-2-phenylindole staining analysis and polymerase chain reaction (PCR were performed in symptomatic and asymptomatic plants. Positive PCR samples were sequenced to identify the pathogens involved. In individuals of both species with witches’ broom symptoms, DAPI staining showed fluorescent bodies in the phloem tissues, but not in asymptomatic plants. Verification by nested-PCR, phytoplasmatic DNA was amplified from diseased murta and chaura, but not in apparently healthy plants. Sequencing of amplified products allowed locating phytoplasma within the ash yellows group (16SrVII and related to Candidatus phytoplasma fraxini. This is the first report of phytoplasma in Chilean native species. Considering the diversity of plant species infected by the ash yellows group suggests that G. phillyreifolia and U. molinae could be a phytoplasma reservoir for other economically important agricultural crops.La murta (Ugni molinae Turcz. y la chaura común (Gaultheria phillyreifolia (Pers. Sleumer son especies nativas de Chile. En plantas de ambas especies se ha observado una sobre-ramificación de tipo "escoba de bruja". En muchas plantas los agentes causales de esta sintomatología son fitoplasmas. Para verificar la presencia de estos microorganismos se analizaron plantas con y sin síntomas mediante tinciones DAPI (4’,6-diamidino-2-fenilindol y reacción en cadena de la polimerasa (PCR. Muestras positivas en la PCR fueron secuenciadas para identificar al fitopatógeno implicado. En individuos de ambas especies con síntomas de escoba de bruja, la tinción DAPI permitió observar cuerpos fluorescentes en los tejidos del floema, situaci

  5. Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data Avaliação da diversidade genética em uma amostra de acessos de cacau resistentes à doença vassoura-de-bruxa, com base em dados de RAPD e pedigree

    Directory of Open Access Journals (Sweden)

    Ronaldo Carvalho dos Santos

    2005-01-01

    Full Text Available Genetic diversity in cocoa (Theobroma cacao L. has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16 primers and pedigree information was obtained from the International Cocoa Germplasm Database. Genetic similarities, genetic distances and coefficient of parentage were calculated using available software. Relatively low genetic diversity was observed in this germplasm set, probably because of great genetic relatedness amongst accessions studied and the poor representation of the germplasm. The TSA-644 descendants were more diverse than the other accessions used in the study. This might be due to the origin of the TSA clone, which was derived from highly divergent genotypes. Association between genetic similarities based on RAPD data and coefficient of parentage, based on pedigree data, was very low, probably due to the homogeneity of the breeding stocks and poor pedigree information. These findings are useful to cocoa breeders in planning crosses for the development of hybrid and clonal cultivars.A diversidade genética em cacau (Theobroma cacao L., embasada em dados morfológicos e em marcadores moleculares, tem sido avaliada com fins de manejo de germoplasma e uso no melhoramento genético. Dados de genealogia de cacau, embora disponíveis, não têm sido utilizados. Foi analisada a

  6. 中国各地不同枣树品种上枣疯病植原体的PCR检测及分子变异分析%Molecular detection and variability of jujube witches'-broom phytoplasmas from different cultivars in various regions of China

    Institute of Scientific and Technical Information of China (English)

    徐启聪; 田国忠; 王振亮; 孔繁华; 李永; 王合

    2009-01-01

    [Objective] Jujube witches'-broom is an important disease in jujube cultivation areas, which causes serious losses in jujube fruit production. To understand the genetic variability and diversity of jujube witches'-broom phytoplasma population from the different cultivars and various regions of China. [Method] We collected 32 samples from 14 cultivars or wild sour jujubes in 7 regions of China and detected them with PCR with the primers RI6mF2/R16mR1 for phytoplasma 16S rDNA, SRI/SR for16S23SrRNA space region (SR) and FDgf/r for secretion proteins (secY). The direct sequencing of PCR products and sequencing by cloned PCR products were used for sequence polymorphism and phylogenetic analyses by comparison to the databases of known conserved gene sequences. [Results] We detected phytoplasmas by PCR amplification of 16SrDNA from all the diseased jujube samples. All the phytoplasma isolates infected various jujube cuhivars belonged to subgroup 16SrV-B of elm yellows group and had closer homology with Bischofia polycarpa witches'-broom and cherry lethal yellows phytoplasmas occurred in China than other 16SrV phytoplasmas in other countries. The sequence polymorphism at different extent in 16SrDNA, SR and secY gene and genetic diversity were revealed in phytoplnsma strain population related to different habitats, among which the dominant strains were always detected by the direct sequencing of PCR products in all the diseased areas of China. The degree of variability on secY gene of collected phytoplasma strains was greater than that of 16SrDNA and SR sequences, and some base substitutions could not alter encoded amino acid, however certain single base deletions detected in a Shandong and a Beijing strains may have impact on the gene structure or function. [Conclusion] Phytoplasma strains from different cultivars and regions show dramatic genetic diversity. Compared with direct sequencing of PCR products, the sequencing by cloning PCR products was more useful for the

  7. Cloning, expression and characterization of tRNA-isopentenyltransferase genes (tRNA-ipt) from paulownia witches'-broom phytoplasma%泡桐丛枝植原体tRNA异戊烯基焦磷酸转移酶基因克隆、原核表达及功能分析

    Institute of Scientific and Technical Information of China (English)

    胡佳续; 田国忠; 林彩丽; 宋传生; 牟海青; 任争光; 郭颂; 周涛; 范在丰

    2013-01-01

    [目的]为了鉴定植原体tRNA异戊烯基焦磷酸转移酶基因(tRNA-ipt)的表达及蛋白功能,探索植原体致病机理.[方法]对泡桐丛枝、桑萎缩、长春花绿变及苦楝丛枝植原体tRNA-ipt基因完整序列进行PCR扩增和生物信息学分析.对泡桐丛枝植原体tRNA-ipt基因进行原核表达并制备抗体.利用Western blot和FITC间接免疫荧光显微镜检测其在植原体中的表达.使用分光光度计分析该基因对大肠杆菌生长的影响,用ELISA测定转化菌株细胞分裂素含量.[结果]首次发现泡桐丛枝、桑萎缩、长春花绿变及苦楝丛枝植原体中完整tRNA-ipt基因,大小为876 bp,编码291个氨基酸,且N端均含有ATP/GTP结合位点保守序列(GPTASGKT).4种植原体tRNA-IPT之间的氨基酸序列相似率为99.1%-99.5%,与同组植原体同源性在95.4%-99.3%,与其他组植原体同源性低于70%.SDS-PAGE结果显示tRNA-IPT蛋白在大肠杆菌中得到表达.首次获得泡桐丛枝植原体tRNA-IPT抗体并检测到该蛋白在泡桐发病组织中的特异表达.经过对转化菌株生长曲线及玉米素含量的测定,发现该基因能促进大肠杆菌后期生长和玉米素核苷的积累.[结论]4种植原体tRNA-ipt基因编码相同特性的功能蛋白,泡桐丛枝植原体tRNA-IPT蛋白能够在植原体中表达,根据该基因对异源菌株生长速率和激素合成的影响推断该蛋白可能参与植原体的细胞分裂素合成,在致病过程中起到重要作用.%[Objective] To identify the tRNA-ipt gene of phytoplasmas and analyze the relationship between tRNA-ipt and synthesis of cytokinin as well as pathogenesis in phytoplasmas.[Methods] The paulownia witches'-broom phytoplasma (PaWB) tRNA-ipt gene was expressed in E.coli and specific antibody was prepared.Then the growth curve and cytokinin contents of E.coli with PaWB tRNA-ipt were measured by photodensitometry and ELISA respectively.[Results] The length of tRNA-ipt genes from PaWB as

  8. Evolução da vassoura-de-bruxa e avaliação da resistência em progênies de cupuaçuzeiro Evolution of the witch's broom disease and evaluation of resistance in cupuassu progenies

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2009-12-01

    Full Text Available Com o objetivo de conhecer a evolução da doença vassoura-de-bruxa, a taxa de segregação e estimar parâmetros genéticos, foi conduzido um experimento com 21 progênies de irmãos completos de cupuaçuzeiro, instalado em Belém, Pará. As progênies foram obtidas pelo cruzamento controlado de clones resistentes com clones resistentes, clones resistentes com clones suscetíveis e, clones suscetíveis com clones suscetíveis. Foram avaliadas também três progênies de meios-irmãos como testemunhas. Os experimentos foram avaliados ao nível de indivíduos, e as análises foram conduzidas via metodologia de modelos lineares mistos (procedimento REMl/BlUP, como delineamento em blocos incompletos desbalanceados, com tratamentos comuns entre dois experimentos. As variáveis avaliadas foram: percentagem de plantas resistentes ao ataque da doença no ramo, inflorescência, fruto imaturo e maduro, no período de 2002 a 2007. Os resultados mostraram que a emissão de vassoura vegetativa é especialmente importante entre julho e setembro. A evolução da doença evidencia que as podas fitossanitárias devem ser realizadas no final da safra, nos meses de maio/junho, e repassadas em setembro/outubro. A população estudada oferece excelente possibilidade de seleção e ganho genético, respaldada pelos elevados índices de variabilidade genética e herdabilidade dos caracteres de resistência. As progênies segregaram tanto para os sintomas de vassoura nos ramos, nas inflorescências, como nos frutos imaturos e maduros. Para o controle integrado desta doença, os resultados mostram a importância da associação de materiais genéticos resistentes ou moderadamente resistentes à vassoura-de-bruxa com as podas fitossanitárias.This paper aimed to study the evolution and individual segregation of the witch's broom disease and estimate the genetic parameters in trials with 21 full sib families of Cupuaçú established in Belém, Pará. The progenies were

  9. Impact of Annual Temperature Variation on Distribution and Year-round Concentration Variation of Paulownia Witches' Broom Phytoplasma%周年温度变化对泡桐丛枝病植原体分布和消长的影响

    Institute of Scientific and Technical Information of China (English)

    曹亚兵; 翟晓巧; 介大委; 范国强

    2016-01-01

    To explore the effect of temperature variation on the distribution of witches' broom phytoplas-ma and its annual changes of relative content in paulownia,direct-RCR and nest-PCR were used to re-search the seasonal variation of the phytoplasma content and distribution. Our result showed that the distri-bution of phytoplasma in varying degree of diseased paulownia was different,meanwhile the concentration in different organs of the same plant was not the same. In the moderately diseased plant,phytoplasma sur-vived all the year round in branches and increased gradually with the rising of temperature. The highest concentration of phytoplasma appeared in August, and subsequently, the concentration of phytoplasma gradually decreased. The similar situation appeared in leaves,except that the concentration of phytoplasma reached the maximum in July and the minimum in October. In root, the phytoplasma concentration was lower and changed less than in leaves and branches,which reached the maximum in September,and then reduced. In all,the relationship between the variation of phytoplasma concentration in paulownia and an-nual changes of temperature was very close.%为探讨温度变化对泡桐树体内丛枝病植原体分布和消长的影响,利用巢式PCR和直接PCR研究了植原体在泡桐不同器官内的分布及相对含量的周年变化.结果表明,不同发病程度泡桐中丛枝病植原体的分布不同,发病程度相同泡桐不同器官内植原体含量也存在一定差异.在中等发病程度的泡桐中,丛枝病植原体全年存在于枝条内,并且其含量随温度升高逐渐升高,8月份达到最高,此后开始下降;叶片内植原体含量随温度升高明显增加,7月份含量最高,随后减少,10月份降到最低;根部植原体含量随温度升高也相应增加,在9月份达到最高,之后开始降低,全年含量变化较小,且含量最高值较叶片和枝条中低.表明泡桐丛枝病植原体在寄主体内的消长与

  10. Aksenoks proves true to "new broom" theory / Aaron Eglitis

    Index Scriptorium Estoniae

    Eglitis, Aaron

    2005-01-01

    Riia linnapea Aivars Aksenoks tühistas linnas hasartmängukeelu, väites, et see oli eelmise linnapea Gundars Bojarsi valimiseelne kampaania. Aksenoks tegi riigi korruptsioonivastase võitluse büroole ettepaneku uurida eelmise Riia linnapea ja välireklaamifirma Clear Channel Latvia vahelist tehingut

  11. 3-D surface scan of biological samples with a push-broom imaging spectrometer

    Science.gov (United States)

    The food industry is always on the lookout for sensing technologies for rapid and nondestructive inspection of food products. Hyperspectral imaging technology integrates both imaging and spectroscopy into unique imaging sensors. Its application for food safety and quality inspection has made signifi...

  12. A new broom? The space agency faces a shake-up

    CERN Multimedia

    2001-01-01

    The results of a recent report have pointed a grim picture of the agency's mismanagement of the ISS. The newly appointed administrator of NASA is likely to implement some combination of mission cancellations and staff lay-offs to cut costs (1/2 page)

  13. UNIFORM FARM OPERATIONS (UFO ON HEMP BROOM RAPE SEED GERMINATION BY BIOLOGICAL CONTROL MANAGEMENT IN IRAN

    Directory of Open Access Journals (Sweden)

    Behzad SANI

    2014-06-01

    Full Text Available Weeds are a constant problem in agronomy and they not only compete with crops for water, nutrients, sunlight, andspace but also harbor insect and disease pests; clog irrigation and drainage systems; undermine crop quality; anddeposit weed seeds into crop harvests. In order to the microbial herbicide (Orocide influence on seed germinationin Orobancheramosa L., this experiment was conducted in 2011 at Islamic Azad University Shahr-e-Qods Branch inTehran by a completely randomized design with four replications. The factor studied included use of Orocide(0(T1, 2(T2, 4(T3 and 6(T4 percentage. The results showed that the effect of microbial herbicide (Orocide wassignificant on germination percentage of Orobancheramosa. Mean comparison showed that the highest germinationpercentage (79% was achieved by non-application of Orocide and lowest germination percentage (8% wasachieved by application of 4% Orocide.The results of this experiment showed that the use of Orocide can decreasedthe germination in Orobancheramosa L. that is uniform farm operations (UFO very important for weed biologicalcontrol management at Iran.

  14. Multibeam collection for FK150324: Multibeam data collected aboard Falkor from 2015-03-24 to 2015-04-06, departing from Broome, Australia and returning to Broome, Australia

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  15. New information on the braincase and inner ear of Euparkeria capensis Broom: implications for diapsid and archosaur evolution

    Science.gov (United States)

    Sobral, Gabriela; Sookias, Roland B.; Bhullar, Bhart-Anjan S.; Smith, Roger; Butler, Richard J.; Müller, Johannes

    2016-07-01

    Since its discovery, Euparkeria capensis has been a key taxon for understanding the early evolution of archosaurs. The braincase of Euparkeria was described based on a single specimen, but much uncertainty remained. For the first time, all available braincase material of Euparkeria is re-examined using micro-computed tomography scanning. Contrary to previous work, the parabasisphenoid does not form the posterior border of the fenestra ovalis in lateral view, but it does bear a dorsal projection that forms the anteroventral half of the fenestra. No bone pneumatization was found, but the lateral depression of the parabasisphenoid may have been pneumatic. We propose that the lateral depression likely corresponds to the anterior tympanic recess present in crown archosaurs. The presence of a laterosphenoid is confirmed for Euparkeria. It largely conforms to the crocodilian condition, but shows some features which make it more similar to the avemetatarsalian laterosphenoid. The cochlea of Euparkeria is elongated, forming a deep cochlear recess. In comparison with other basal archosauromorphs, the metotic foramen is much enlarged and regionalized into vagus and recessus scalae tympani areas, indicating an increase in its pressure-relief mechanism. The anterior semicircular canal is extended and corresponds to an enlarged floccular fossa. These aspects of the braincase morphology may be related to the development of a more upright posture and active lifestyle. They also indicate further adaptations of the hearing system of Euparkeria to terrestriality.

  16. New information on the braincase and inner ear of Euparkeria capensis Broom: implications for diapsid and archosaur evolution

    Science.gov (United States)

    Sookias, Roland B.; Bhullar, Bhart-Anjan S.; Smith, Roger; Butler, Richard J.; Müller, Johannes

    2016-01-01

    Since its discovery, Euparkeria capensis has been a key taxon for understanding the early evolution of archosaurs. The braincase of Euparkeria was described based on a single specimen, but much uncertainty remained. For the first time, all available braincase material of Euparkeria is re-examined using micro-computed tomography scanning. Contrary to previous work, the parabasisphenoid does not form the posterior border of the fenestra ovalis in lateral view, but it does bear a dorsal projection that forms the anteroventral half of the fenestra. No bone pneumatization was found, but the lateral depression of the parabasisphenoid may have been pneumatic. We propose that the lateral depression likely corresponds to the anterior tympanic recess present in crown archosaurs. The presence of a laterosphenoid is confirmed for Euparkeria. It largely conforms to the crocodilian condition, but shows some features which make it more similar to the avemetatarsalian laterosphenoid. The cochlea of Euparkeria is elongated, forming a deep cochlear recess. In comparison with other basal archosauromorphs, the metotic foramen is much enlarged and regionalized into vagus and recessus scalae tympani areas, indicating an increase in its pressure-relief mechanism. The anterior semicircular canal is extended and corresponds to an enlarged floccular fossa. These aspects of the braincase morphology may be related to the development of a more upright posture and active lifestyle. They also indicate further adaptations of the hearing system of Euparkeria to terrestriality. PMID:27493766

  17. Leachate treatment system using constructed wetlands, Town of Fenton sanitary landfill, Broome County, New York. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1993-11-01

    Municipal sanitary landfills generate leachate that New York State regulations require to be collected and treated to avoid contaminating surface water and groundwater. One option for treating leachate is to haul it to municipal wastewater treatment facility. This option may be expensive, may require excessive energy for transportation, and may require pretreatment to protect the receiving facility`s processes. An alternative is on-site treatment and discharge. Personnel from the Town of Fenton, New York; Hawk Engineering, P.C.; Cornell University; and Ithaca College designed, built, and operated a pilot constructed wetland for treating leachate at the Town of Fenton`s municipal landfill. The system, consisting of two overland flow beds and two subsurface flow beds has been effective for 18 months in reducing levels of ammonia (averaging 85% removal by volatilization and denitrification) and total iron (averaging 95% removal by precipitation and sedimentation), two key constituents of the Fenton landfill`s leachate. The system effects these reductions with zero chemical and energy inputs and minimal maintenance. A third key constituent of the leachate, manganese, apparently passes through the beds with minimal removal. Details and wetland considerations are described.

  18. The Scotch broom, Cytisus scoparius (Fabaceae), a paradox in Denmark – an invasive plant or endangered native species?

    DEFF Research Database (Denmark)

    Rosenmeier, Lars; Kjær, Erik Dahl; Nielsen, Lene Rostgaard

    2013-01-01

    plants showed that the two gene pools correspond to populations exhibiting either a horizontal habit and non-invasive behaviour or an erect habit and, in some cases, invasive behaviour. The Italian population clustered with the erect ones. We discuss the origin and taxonomic status of the two gene pools...

  19. Genetic structure and population differentiation of the Mediterranean pioneer spiny broom Calicotome villosa across the Strait of Gibraltar

    DEFF Research Database (Denmark)

    Arroyo, Juan; Aparicio, Abelardo; Albadalejo, Rafael G.;

    2008-01-01

    of species during the Pleistocene, as a consequence of the lowering of sea level and climate changes associated with the Ice Ages. In the present study, we report a multilevel hierarchical investigation of the genetic diversity of Calicotome villosa, a common pioneer legume shrub, at the regional scale......The region around the Strait of Gibraltar is considered to be one of the most relevant ‘hot spots’ of biodiversity in the Mediterranean Basin due to its historical, biogeographical, and ecological features. Prominent among these is its role as a land bridge for the migration and differentiation...

  20. Strategi Pengembangan Usaha Mikro Kecil Dan Menengah (Studi Kasus Kerajinan Sapu Moro Bondo di Desa Limau Manis, Kecamatan Tanjung Morawa, Kabupaten Deli Serdang)

    OpenAIRE

    Pakpahan, Radius

    2014-01-01

    Micro, Small and Medium Enterprises which in recent years has continued to grow and thrive in Deli Serdang is a craft broom. One of SMEs engaged in craft and production efforts Moro Bondo broom is located at Pasar 14 Village Limau Manis, District of Tanjung Morawa, Deli Serdang. This effort established in 1986 to produce broom fibers and ceased operations in 2004. In 2008, these businesses operate again until today producing fibers broom, mop and broom plastic. The purpose of this study is...

  1. Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao

    Directory of Open Access Journals (Sweden)

    Francis Julio Fagundes Lopes

    2008-02-01

    Full Text Available The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular, the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts and transformation efficiency. In addition, the time necessary for the detection of the first transformants and the number of insertions were reduced.A presença de enzima de restrição na mistura de transformação aumentou a eficiência da transformação em Moniliophthora perniciosa. A influência da forma do vetor (linear ou circular, o padrão de integração do plasmídeo nos sítios genômicos e a influência do promotor usado para expressar o gene marcador foram também analisados. A adição de BamHI ou NotI aumentou o número de transformantes 3-10 vezes e 3 vezes, respectivamente, em relação ao controle sem a adição da enzima. O uso de plasmídeos pré-linearizados não aumentou a eficiência da transformação quando comparado à eficiência obtida com plasmídeos circulares. No entanto, a freqüência de transformantes multi-cópias aumentou significativamente. Juntos os procedimentos reportados aqui resultaram em processos mais eficientes de produção de protoplastos e transformação, onde o tempo necessário para o aparecimento dos transformantes e o número de inserções múltiplas foi reduzido.

  2. 烟草丛顶病中间寄主测定%Identification of Intermediate Host of Tobacco Witches' Broom Disease

    Institute of Scientific and Technical Information of China (English)

    谭仲夏; 秦西云; 杨龙祥

    2003-01-01

    采用饲毒烟蚜(Myzus persicae Sulzer)和汁液摩擦传毒的方法,对22种烟蚜田间常见的中间寄主进行接种,接种后7d,用无毒烟蚜接种中间寄主饲养2d后回接到健康烟株.观察烟株是否发病.经多次重复试验,最终确定小酸浆(Physalis angulata L)和龙葵(Datura stramonium L)为烟草丛顶病的中间寄主.采用汁液传毒中间寄主并用蚜虫回接烟苗未出现发病症状.

  3. Hydrogeology of the Susquehanna River valley-fill aquifer system in the Endicott-Vestal area of southwestern Broome County, New York

    Science.gov (United States)

    Randall, Allan D.; Kappel, William M.

    2015-07-29

    The village of Endicott, New York, and the adjacent town of Vestal have historically used groundwater from the Susquehanna River valley-fill aquifer system for municipal water supply, but parts of some aquifers in this urban area suffer from legacy contamination from varied sources. Endicott would like to identify sites distant from known contamination where productive aquifers could supply municipal wells with water that would not require intensive treatment. The distribution or geometry of aquifers within the Susquehanna River valley fill in western Endicott and northwestern Vestal are delineated in this report largely on the basis of abundant borehole data that have been compiled in a table of well records.

  4. National Dam Safety Program. Little Choconut Watershed Site 2B Dam (Inventory Number 721), Susquehanna River Basin, Broome County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-06-30

    appear that about 4,000 cu. yds. may be quite durable as rock fill. B. Compacted Dry Density: Standard Proctor compaction tests ( ASTM D-698) on the minus...O%) //,E. 6ZL ___ 0.2 . 6-2 /4.’-./ _ DEVIATOR STRESS (-, -a- 3 ) psi 0 0 4 . ’/A 0. SHA ARMTR t deg NORMA STES(o) REMRK ApeAC rr7 o,->-~~9~ S~~ ~ 0...PARTICLE SIZE INCLUDED IN TEST -W "STD. (AST M 0 - 698) 2: METHOD A SPCFCGAIY( f MINUS NO. 4 ~’ 4MOD.( ASTM 0-6557)0; METHOD____ SPEIFC RAIT ( 8)PLUS NO. 4

  5. Structural studies of alternative oxidase (AOX) from moniliophthora perniciosa, the causal agent of witches' broom disease in cacao: a membrane-associated protein

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, J.F.; Prado, P.F.V.; Tiezzi, H.O.; Dias, S.M.G.; Ambrosio, A.L.B. [Laboratorio Nacional de Biociencias - LNBIO, Campinas, SP (Brazil); Thomazella, D.P.T.; Teixeira, P.J.P.L.; Pereira, G.A.G. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil)

    2012-07-01

    Full text: Alternative oxidase (AOX) is a protein attached to the inner mitochondrial membrane that receives electrons directly from reduced ubiquinone and catalyzes the reduction of oxygen to water. AOX is a non-proton motive terminal quinol oxidase that enables cell respiration to continue even in the presence of inhibitors targeting the complexes of the respiratory chain. This protein is present in higher plants, pathogenic fungi and some parasites. The structural characterization of AOX becomes interesting due to its potential as a fungicide target. AOX is predicted to be a monotopic interfacial membrane protein interacting with a single leaflet of the lipid bilayer, rather than transmembrane. Amino acid sequence analysis reveals the presence of two conserved glutamate-histidine motifs, identifying it as a member of the diiron carboxylate protein family. The AOX model is defined by two pairs of helices forming a four helix bundle and an additional hydrophobic connecting sequence between the two helical pairs is proposed to act as the membrane anchoring region. In this work we aim at production, purification and crystallization of the AOX protein from M. perniciosa for further structural studies of this membrane-associated protein, by X-ray protein crystallography (author)

  6. Introduced populations of Genista monspessulana (French broom) are more dense and produce a greater seed rain in California, USA, than native populations in the Mediterranean Basin of Europe

    Science.gov (United States)

    Some invasive plants perform better in their area of introduction than in their native region. The increased vigor of invasive plants can result from phenotypic plasticity in response to more favorable environmental conditions or release from natural enemies, or from adaptive evolution of increase...

  7. First report of 16SrII-C subgroup phytoplasma causing phyllody and witches’-broom disease in Soybean in Tanzania

    NARCIS (Netherlands)

    Murithi, H.; Owati, A.; Madata, C.S.; Joosten, M.; Beed, F.; Lava Kumar, P.

    2015-01-01

    Soybean production in Tanzania is steadily increasing, driven by growing demand from feed and livestock producers and also for human consumption. Soybean production area has increased from 795 ha in 2003 to 4,100 ha in 2013 (FAO 2014). Major soybean production is in the Morogro, Ruvuma, Iringa, and

  8. Fruit Trees and Tamarisk Brooms: Grafting a Unique Perspective of American History in Willa Cather’s Death Comes for the Archbishop

    Science.gov (United States)

    2007-11-02

    were experiences of [her] own" (8). She claims that Death Comes for the Archbishop, notable for its lack of driving plot, is a "special genre ," arguing...9 May 05). Murphy, John J. "Willa Cather." Ed. in Chief, J. Golden Taylor. A Literary History of the American West. <http://www2.tcu.edu/depts/prs...Dear AFIT PA, I am an English Literature PhD student (and public affairs officer ©). I have a literary paper I am presenting at a conference in June

  9. National Dam Safety Program. Little Choconut Watershed Site 2A Dam (Inventory Number NY 720), Susquehanna River Basin, Broome County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-06-30

    in the Choco - nut and Susquehanna valleys. Till, associated with theI glacial advance, mantles the rest of the site. b. Subsurface Investigations 1...basis of the visual examination, the Little Choco - nut Watershed Site 2A Dam is considered to be in good condition. There were no signs of impending

  10. Effects of Antibiotics on the Paulownia Witches' Broom Phytoplasmas and Pathogenic Protein Related to Witches' Broom Symptom%抗生素对泡桐丛枝病植原体和发病相关蛋白质的影响

    Institute of Scientific and Technical Information of China (English)

    范国强; 张胜; 翟晓巧; 刘飞; 董占强

    2007-01-01

    泡桐(Paulownia sp.)是我国重要的速生用材和绿化树种;然而,丛枝病的发生给我国的泡桐生产带来了巨大的损失。自从泡桐丛枝病病原菌被发现以来,人们对其发病机理和防治方法进行了大量研究(李秀生,1983;任国兰等,1991;李加友,1997;范国强等,2003a;2006;何放亭等,1997;田国忠等,1994;王蕤等,1981),但由于其病原菌——植原体自身的特性,至今国内外仍不能进行体外活体培养,因此,限制了泡桐丛枝病有效防治方法的建立。众所周知,蛋白质是基因表达的最终产物,基因的信息通过mRNA转录再到合成的蛋白质。

  11. Multibeam collection for FK150410: Multibeam data collected aboard Falkor from 2015-04-10 to 2015-05-04, departing from Broome, Australia and returning to Darwin, Australia

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  12. Una Escoba para Ana, Cien Oficios para Juan. Quinto Modulo de una Serie para Maestros de Escuela Elemental. (A Broom for Anna, A Hundred Jobs for John. Fifth Module of a Series for Elementary School Teachers).

    Science.gov (United States)

    Molina, Carmen Eneida, Ed.; And Others

    This guide for teachers, in English and Spanish, examines the stereotyped work roles assigned to men and women. The guide examines educational materials that perpetuate these roles and presents teaching alternatives which reinforce students' self esteem and confidence. A pre-test and post-test are included to measure the user's awareness of…

  13. Candidatus Phytoplasma brasiliense associado ao superbrotamento do hibisco (Hibiscus rosa-sinensis L. no Estado de São Paulo Candidatus Phytoplasma brasiliense associated with hibiscus witches' broom in the State of São Paulo-Brazil

    Directory of Open Access Journals (Sweden)

    Eliane Gonçalves da Silva

    2009-09-01

    Full Text Available Plantas de hibisco com superbrotamento e definhamento seguido de morte têm sido observadas nos municípios de São Paulo, Campinas e Piracicaba. Como os sintomas são sugestivos daqueles induzidos por fitoplasmas, o presente trabalho buscou identificar o possível fitoplasma associado com a doença. Assim, 14 plantas sintomáticas de hibisco foram coletadas em Piracicaba (SP e submetidas ao PCR duplo com os primers P1/Tint-R16F2n/R2 e ao exame em microscópio eletrônico de transmissão. A identificação foi realizada por análise de RFLP com as enzimas de restrição BfaI, DraI, HaeIII, HhaI, HpaII, MboI, MseI, RsaI e TaqI. Testes de transmissão foram conduzidos com enxertia de ramos e uso de Cuscuta subinclusa. Os resultados de nested-PCR revelaram a presença consistente de fitoplasmas em todas as plantas sintomáticas e foram confirmados pela observação de corpúsculos pleomórficos no floema, através da microscopia eletrônica. A análise de RFLP mostrou que o fitoplasma encontrado em hibisco pertence ao grupo 16SrXV, o mesmo grupo do Candidatus Phytoplasma brasiliense. O fitoplasma foi transmitido de planta doente para sadia, tanto pela enxertia como pela C. subinclusa, demonstrando ser o agente do superbrotamento do hibisco.Ornamental hibiscus have been affected by shoot proliferation and decline followed by death in several cities in São Paulo State, especially São Paulo, Campinas and Piracicaba. As the symptoms are suggestives of those induced by phytoplasmas, the present work aimed to identify the possible phytoplasma associated with the disease. Fourteen symptomatic hibiscus were sampled in Piracicaba, submitted to nested-PCR with the primers P1/Tint-R16F2n/R2 and processed by transmission electron microscopy. The identification was made by RFLP analyses with the restriction enzymes BfaI, DraI, HaeIII, HhaI, HpaII, MboI, MseI, RsaI, and TaqI. Transmission assays were performed by grafting and Cuscuta subinclusa. The presence of phytoplasma was always detected by molecular technique and electron microscopy in all samples. The RFLP analyses showed that the phytoplasma belongs to group 16SrXV, the same group of the Candidatus Phytoplasma brasiliense. It was experimentally transmitted by C. subinclusa to periwinkle and by grafting to hibiscus in which it reproduced the original symptoms. This indicates that the studied phytoplasma is the causal agent the disease.

  14. 抗枣疯病枣树品种的DAPI荧光染色检测%Detection of resistent varieties of jujube trees to jujube witches-broom disease with DAPI fluorescence staining

    Institute of Scientific and Technical Information of China (English)

    郭晓军; 田国忠; 赵少波; 李树卿

    2003-01-01

    该文通过嫁接枣疯病病皮和病枝传病的方法,对抗病材料婆枣(JL)和壶瓶枣(Hu)共10棵单株,结合感病酸枣(Suan)品种的健枝和疯枝作对照,应用DAPI染色技术对抗病材料体内植原体的侵染情况进行了荧光观察,结果表明:抗病植株体内已感染了植原体病原,说明应用嫁接方法进行枣疯病病原接种是可行的,但其却不表现丛枝症状,证明了抗病材料具有一定的抗病性;同时,抗病材料中发现的金黄色自发荧光,也为其抗病物质的寻找和抗病机理的研究提供了重要线索.

  15. Tc-cAPX, a cytosolic ascorbate peroxidase of Theobroma cacao L. engaged in the interaction with Moniliophthora perniciosa, the causing agent of witches' broom disease.

    Science.gov (United States)

    Camillo, Luciana Rodrigues; Filadelfo, Ciro Ribeiro; Monzani, Paulo Sérgio; Corrêa, Ronan Xavier; Gramacho, Karina Peres; Micheli, Fabienne; Pirovani, Carlos Priminho

    2013-12-01

    The level of hydrogen peroxide (H2O2) in plants signalizes the induction of several genes, including that of ascorbate peroxidase (APX-EC 1.11.1.11). APX isoenzymes play a central role in the elimination of intracellular H2O2 and contribute to plant responses to diverse stresses. During the infection process in Theobroma cacao by Moniliophthora perniciosa oxidative stress is generated and the APX action recruited from the plant. The present work aimed to characterize the T. cacao APX involved in the molecular interaction of T. cacao-M. perniciosa. The peroxidase activity was analyzed in protein extracts from cocoa plants infected by M. perniciosa and showed the induction of peroxidases like APX in resistant cocoa plants. The cytosolic protein of T. cacao (GenBank: ABR68691.2) was phylogenetically analyzed in relation to other peroxidases from the cocoa genome and eight genes encoding APX proteins with conserved domains were also analyzed. The cDNA from cytosolic APX was cloned in pET28a and the recombinant protein expressed and purified (rTc-cAPX). The secondary structure of the protein was analyzed by Circular Dichroism (CD) displaying high proportion of α-helices when folded. The enzymatic assay shows stable activity using ascorbate and guaiacol as an electron donor for H2O2 reduction. The pH 7.5 is the optimum for enzyme activity. Chromatographic analysis suggests that rTc-cAPX is a homodimer in solution. Results indicate that the rTc-cAPX is correctly folded, stable and biochemically active. The purified rTc-cAPX presented biotechnological potential and is adequate for future structural and functional studies.

  16. Diversity of Resistance to Jujube Witches’ Broom Disease Among the Strains of Ziziphus jujuba Mill. Junzao%骏枣不同株系间的枣疯病抗性多样性

    Institute of Scientific and Technical Information of China (English)

    肖京; 杨艳荣; 赵锦; 刘孟军

    2013-01-01

    [Objective]Sixteen strains of Z. jujuba Junzao were collected and evaluated, aiming at revealing the diversity of resistance to JWB within cultivar, screening excellent strains with high resistance. [Method]The JWB symptom development and phytoplasma infection were studied through field investigation and PCR analysis. The fruit economical characters, including the rate of water content, fruit weight, fruit shape index, ratio of edibility, sugar content, Vc content and titratable acidity, were comprehensively evaluated among different strains using synthesis-reasonable satisfaction and multivalue theory merging rule. The genetic diversity of Junzao strains were analyzed by AFLP. [Result] Different strains of Junzao showed significant differences in JWB resistance. According to the situations of disease symptom development and phytoplasma infection, the strains were divided into four groups:sensitive, delayed sensitive, delayed resistant and resistant, and 4 strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out. In the resistant group, the fruit quality of T15, J5 and J8 was also at higher level among the sixteen strains. AFLP analysis showed that there had significant differences in DNA level among different strains of Junzao.[Conclusion] Four strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out, and three of them (T15, J5 and J8) also have an excellent fruit quality.%[目的]揭示骏枣品种内枣疯病抗性多样性,筛选高抗枣疯病的骏枣株系。[方法]以骏枣的16个株系为试材,通过田间调查和PCR检测,研究不同株系在嫁接侵染枣疯病后的症状表现和病原侵染情况;利用“合成-合理满意度”和多维价值理论的合并原则,对不同株系果实的相对含水率、单果重、可食率、果形指数、Vc含量、可溶性糖和可滴定酸含量等性状指标进行综合评价;采用AFLP技术对不同株系进行基因组遗传多样性分析。[结果]不同株系对枣疯病抗性表现出显著差异,可分为感病型、延迟感病型、延迟抗病型和抗病型4种类型, T15、J2、J5、J8这4个骏枣株系高抗枣疯病,其中,T15、J5和J8的果实综合性状在骏枣株系中亦居于较高水平;进一步的AFLP分析发现,不同抗性类型间在基因组水平上存在一定差异。[结论]筛选出了4个抗枣疯病骏枣株系,其中3个株系果实综合品质良好。

  17. 敏捷光学卫星密集区域推扫成像任务规划方法%Push-broom Imaging Scheduling Method of the Optical Agile Satellite in the Dense Regions

    Institute of Scientific and Technical Information of China (English)

    林晓辉; 潘小彤; 张锦绣

    2014-01-01

    The agile satellite could achieve the linescan imaging model with the ability of three-axis-maneuvering , w hile imaging synchronously during the maneuvering . Based on the imaging agile satellite task , the model of the problem of scheduling agile earth observing satellite (AEOS) with the linescan imaging was established . Using the Gauss projection , the preliminary method of the linescan strip was established ,and solved by the existing algorithms in the Matlab . Because of the complexity of the model , the solution strategy of the hierarchical optimization was designed .During the sub-problem , generating the linescan stripe with groups , the solution method can be solved easily . In the upper scheduling problem , according to the two directions of the stripe , the hierarchical optimization based on heuristic rule was designed to solve the problem .Then ,turning the stripes that could not be scheduled into the point target , the whole scheduling was completed with the second-time-scheduling . The simulation shows that the algorithm can solve the actual problem of the scheduling of the linescan imaging agile satellite .%敏捷成像卫星可以实现利用三轴姿态机动所形成的推扫成像模式,同时可以在机动过程中同步成像。在敏捷卫星成像任务的基础上,建立考虑推扫成像模式的敏捷卫星任务规划模型。通过高斯投影建立球面直线扫描条带的数学生成模型,并利用Matlab现有函数进行优化解算;由于模型解算的复杂性,将整体规划问题分为两层子问题分别进行处理,在条带任务分配中,设计了适用于计算机计算的含有条带分配策略的解算算法;在上层规划中,基于推扫成像的双向扫描特性,设计了基于启发式规则的敏捷卫星任务规划算法;之后,将不可规划条带拆作孤立点目标进行处理,最后通过再合成处理完成整体规划。仿真结果表明,文章所设计的算法可以有效处理实际情况下的敏捷卫星推扫成像任务规划问题。

  18. Future spaceborne ocean missions using high sensitivity multiple-beam radiometers

    DEFF Research Database (Denmark)

    Skou, Niels; Søbjærg, Sten Schmidl; Kristensen, Steen Savstrup

    2014-01-01

    Design considerations concerning a scanning as well as a push-broom microwave radiometer system are presented. Strict requirements to spatial and radiometric resolution leads to a multiple-beam scanner achieving good sensitivity through integration over many beams, or to a push-broom system where...

  19. 16 CFR 503.2 - Status of specific items under the Fair Packaging and Labeling Act.

    Science.gov (United States)

    2010-01-01

    ..., nylon, etc.). Brooms and mops. Cameras. Chinaware. Christmas light sets. Cigarette lighters. Clothespins... plaques. Photo albums. Pictures. Plastic table cloths, plastic placement and plastic shelf paper. Rubber.... Aluminum foil cooking utensils. Aluminum wrap. Camera supplies. Candles. Christmas decorations....

  20. 78 FR 53456 - Ocean Transportation Intermediary License Reissuances

    Science.gov (United States)

    2013-08-29

    ....: 018461N. Name: Select Aircargo Services, Inc. dba PAC International Logistics Company. Address: 12801...: July 16, 2013. License No.: 018392N. Name: Broom U.S.A., Inc. dba Transcontinental Logistics Neutral...

  1. Dicty_cDB: Contig-U02501-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ) Aster yellows witches'-broom phytoplasma AYWB, co... 38 0.006 12 ( FG289263 ) 1108793295629 New World Scre...FG290297 ) 1108793315558 New World Screwworm Egg 9261 ESTs C... 44 9.4 1 ( FE7256

  2. Spatial distribution of symbiont-bearing dinoflagellates in the Indian Ocean in relation to oceanographic regimes

    DEFF Research Database (Denmark)

    Tarangkoon, Woraporn; Hansen, Gert; Hansen, Per Juel

    2010-01-01

    The spatial distribution of symbiont-bearing dinoflagellates was investigated during a cruise from Cape Town, South Africa to Broome, Australia. Endo- and ectosymbionts were only found in the order Dinophysiales. The genera Ornithocercus, Histioneis, Parahistioneis and Citharistes had cyanobacteria...

  3. Tualatin River - Bump/Brenner Riparian Forest Pre-Restoration Invasive Plant Treatment

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This project seeks to intensively treat a 38–acre subunit of the refuge heavily infested with red hawthorn (Crataegus monogyna), scotch broom (Cytisus scoparius),...

  4. Reframing Teacher Education for Democratic Engagement

    Science.gov (United States)

    Strahley, Lisa; D'Arpino, Tracy

    2016-01-01

    This chapter describes a partnership between teacher education students at SUNY Broome and students at a local elementary school that led to all participants gaining a stronger sense of themselves as civic change agents in their communities.

  5. Entice, engage, endure: adapting evidence-based retention strategies to a new generation of nurses

    OpenAIRE

    Broom, Catherine

    2010-01-01

    Catherine BroomCatherine Broom Consulting LLC, Lake Forest Park, WA, USAAbstract: Across the globe, the prolonged and expanding nursing shortage threatens dire consequences unless health care leaders can develop successful strategies to entice and engage a new generation of nurses. Over the past 3 decades investigational work regarding workforce attributes and the impact of organizational structures and processes has helped define professional nursing environments that successfully attract, s...

  6. Velocity estimation of an airplane through a single satellite image

    Institute of Scientific and Technical Information of China (English)

    Zhuxin Zhao; Gongjian Wen; Bingwei Hui; Deren Li

    2012-01-01

    The motion information of a moving target can be recorded in a single image by a push-broom satellite. A push-broom satellite image is composed of many image lines sensed at different time instants. A method to estimate the velocity of a flying airplane from a single image based on the imagery model of the linear push-broom sensor is proposed. Some key points on the high-resolution image of the plane are chosen to determine the velocity (speed and direction). The performance of the method is tested and verified by experiments using a WorldView-1 image.%The motion information of a moving target can be recorded in a single image by a push-broom satellite.A push-broom satellite image is composed of many image lines sensed at different time instants.A method to estimate the velocity of a flying airplane from a single image based on the imagery model of the linear push-broom sensor is proposed.Some key points on the high-resolution image of the plane are chosen to determine the velocity (speed and direction).The performance of the method is tested and verified by experiments using a WorldView-1 image.

  7. Molecular Detection of Phytoplasma Strains from Several Plants Around Diseased Paulownia Infected with Paulownia Witches'-broom Phytoplasma%泡桐丛枝病病树周围几种植物上植原体的分子检测

    Institute of Scientific and Technical Information of China (English)

    王洁; 田国忠; 徐启聪; 刘永光; 高瑞; 李向东; 竺晓平

    2010-01-01

    [目的]初步明确自然条件下可能感染泡桐丛枝病植原体的寄主植物.[方法]用植原体16S rRNA基因的通用引物,对从泡桐丛枝病病树周围采集的表现黄化、小叶、皱叶、丛枝等症状或无症状的16种植物样品的DNA进行巢式PCR扩增,对所扩增的片段进行序列测定和分析.并利用泡桐从枝病植原体延伸因子的抗血清对部分样品进行间接免疫荧光观察.[结果]巢式PCR结果显示从牛筋草(Eleusine indics)、辣椒(Capsicum annuum)、狗尾草(Setaria viridis)、山药(Dioscorea opposita)、灯笼泡(Physalis angulata)、花生(Arachis hypogaea)及南瓜(Cucurbita moschata)共7种植物样品和阳性对照PaWB菏泽分离物(PaWB-HZ)中均得到了约1.2 kb的特异性片段.序列分析表明这7种植原体分离物和PaWB-HZ属于翠菊黄化组的16SrI-D亚组.对所采集的植原体侵染的寄主植株辣椒、山药、花生、南瓜和泡桐进行间接免疫荧光观察结果显示,仅在PaWB-HZ侵染的泡桐中发现翠绿色特异荧光,在健康泡桐及其它4种分离物侵染的植物中均未检测到明显的植原体特异荧光.[结论]首次从泡桐丛枝病病树周围存在的7种其它植物中检测到植原体,并且测定其植原体16S rDNA核苷酸序列与泡桐丛枝植原体相关基因片段高度同源,初步推测这7种植物可能是泡桐丛枝病植原体自然寄主或是通过昆虫偶尔被感染.

  8. CLONING AND SEQUENCING OF 16S RRNA GENE OF PHYTOPLASMA CWB1 STRAIN ASSOCIATED WITH CACTUS WITCHES'BROOM%仙人掌丛枝病植原体CWB1株系16S rRNA基因克隆及序列分析

    Institute of Scientific and Technical Information of China (English)

    蔡红; 李凡; 孔宝华; 陈海如

    2001-01-01

    对表现丛枝症状的仙人掌植株总DNA进行植原体16S rRNA基因PCR扩增,得到一条约1.5kb的特异片段,表明植株中有植原体存在,将此植原体株系命名为CWB1.把此特异片段与pGEM-T Easy载体连接并转化到大肠杆菌JM109感受态细胞中,通过PCR鉴定、限制性内切酶(Eco RI)酶切分析及核苷酸序列分析,均表明克隆成功.序列分析结果显示,此株系的16S rRNA基因全长1489个碱基,与属于植原体16S rⅡ-C亚组的Faba bean phyllody植原体同源率最高,为99.7%.通过16S rRNA基因核酸序列同源性比较,认为该株系属于16S rⅡ-C亚组,基本确定了其分类地位.

  9. Efeito de nutrientes combinados com indutores de resistência na proteção contra a vassoura-de-bruxa no cacaueiro Effect of nutrients combined with inducers of resistance on the protection of cocoa seedlings against witches' broom

    Directory of Open Access Journals (Sweden)

    Iris Lettiere do Socorro Santos da Silva

    2008-02-01

    Full Text Available Na busca por novos indutores de resistência contra a vassoura-de-bruxa no cacaueiro avaliou-se o efeito de vários nutrientes, acibenzolar-S-metil (ASM e a combinação desses nutrientes com ASM. Os produtos e as misturas foram pulverizados 30 dias antes da inoculação nas mudas do clone SIC-23. Foram utilizados os produtos comerciais Supa-potássio®(silicato de potássio, Hortifós® PK (fosfito de potássio e Broadacre® Mn (sulfato de manganês, testados nas dosagens 2,5 mL; 5,0 mL; 10 mL/L de água, isoladamente ou combinados com ASM (0,2g/L. O experimento foi conduzido no delineamento de blocos ao acaso, no esquema fatorial, com quatro repetições de doze plantas/parcela. A incidência da doença foi avaliada 60 dias após a inoculação. Somente o ASM promoveu controle significativo da vassoura-de-bruxa. Os nutrientes aplicados isoladamente não apresentaram efeito na severidade da doença. Por outro lado, o efeito protetor do ASM desapareceu quando este foi misturado ao Supa-potássio®, na dose de 5 ou 10 mL/L.Aiming at improving the level of induction of resistance in cocoa, various nutrients, acibenzolar-S-methyl (ASM and their combination were tested on cocoa seedlings, clone SIC-23, 30 days before inoculation. The commercial products Supa-potássio® (potassium silicate, Hortifós® PK (potassium phosphite and Broadacre® Mn (manganese sulfate were sprayed at doses of 2.5, 5.0 mL and 10.0 mL per liter of water, combined or not, with ASM® (0.2 g/L. The experiment was set in a randomized block design, in a factorial scheme, with four replicates of twelve plants each. Disease incidence was assessed 60 days after inoculation. Only ASM promoted significant control of the disease. Nutrients alone had no effect on disease incidence. On the other hand, the protective effect of ASM disappeared when this product was mixed to Supa-potássio at 5 or 10 mL/L.

  10. Altertative trophy measuring techniques for African buffalo

    Directory of Open Access Journals (Sweden)

    S.E. Gandy

    2004-12-01

    Full Text Available The African buffalo is considered the classic African trophy. It is the choice of many hunters who will never go on to hunt any of the other dangerous game animals on the continent. A good trophy is perceived as that of a mature bull with a hardened boss and horn tips that lengthen into sharply pointed hooks. However, indications are that these are the bulls in their breeding prime and there is concern that the continued targeting of these individuals will negatively impact on the population dynamics of the herds, ultimately affecting the sustainability of buffalo hunting. As they age and become postreproductive, the horns broom down, reducing the trophy score under the current measurement systems. A new measuring system is needed that encourages hunters to target the older post-reproductive bulls, instead of those that are still breeding. A random sample of trophies was divided into broomed and non-broomed sub-samples. All key parameters that can be measured in the trophy were measured with a view to identifying the parameters that would allow broomed-down individuals to compete favourably with the non-broomed “classic trophy” in the primary measurement systems, those of Safari Club International and Rowland Ward. An index, created through dividing tip space by the mean of the two individual horn lengths proved to serve the purpose. This factor was then applied to the mean of the SCI and Rowland Ward measurements in the samples. These methods allowed broomed horns to score more points in the record books than non-broomed horns. Boss width and boss space are other possible measurement inclusions that could be considered.

  11. Aphandra natalia(Arecaceae – a little known source of piassaba fibers from the western Amazon

    Directory of Open Access Journals (Sweden)

    Mette Kronborg

    2014-03-01

    Full Text Available Aphandra natalia(Balslev & Henderson Barfod is a multipurpose palm that is exploited both commercially and for subsistence purposes. Its fibers are important in Peruvian and Ecuadorean broom industries and support many people economically. In Brazil, it is found in the western part of Acre, where it is the main source for a local broom market. Data from fieldwork in Peru (2007 suggests that the variation in gross profit per kilogram of fiber is considerable among the different segments in the broom industry. Harvesters and distributors earn negligible amounts of money whereas manufacturers reap of the major part of the earnings. Fiber extraction appears to be sustainable in Ecuador and in some parts of Peru, whereas in other parts of Peru unsustainable harvest occurs, involving felling of entire palm trees for the harvest of fibers. The same destructive extraction method is used in Brazil, where the palm is becoming rare in its natural distribution area.

  12. Suggested Planting Guidelines - Best Management Practices for Revegetation of Actively Used Training Lands in the Northeastern United States

    Science.gov (United States)

    2012-09-01

    Carex squarrosa Common name Canada wildrye riverbank wildrye Virginia wildrye broom sedge fox sedge squarrose sedge Fertility Requirement...Fringed brome 5 236,000 27 Fox sedge 2 1,297,000 60 Fowl bluegrass7 2 1,900,000 87 Autumn bentgrass8 1 8,000,000 184 435 Carex scoparia (Optional...yes  Ecological‐bridge suitability:    Additional notes:  Slightly toxic  ERDC/CRREL TR-12-10 90 Broom  sedge   Carex  scoparia Photo by Robert H

  13. On Geometric Correction Method of BJ-1 Panchromatic Image Covering Kingdom of Lesotho

    Institute of Scientific and Technical Information of China (English)

    Shunxi; LIU; Zhongwu; WANG; Wei; HAO; Rongbin; WANG

    2014-01-01

    The purpose is to find a suitable geometric correction method of BJ-1 panchromatic image covering Kingdom of Lesotho.The methods are carrying out two geo-correction experiments based on the push-broom model and the projective transform model for BJ-1 small satellite real panchromatic covering flat and mountain area of Lesotho.Results show that the projective transform model has equal or higher accuracy compared to the push-broom model.Conclusion is the projective transform model can be used in producing land use image map.

  14. Budgeting Facilities Operation Costs Using the Facilities Operation Model

    Science.gov (United States)

    2011-06-01

    composting are included. Excludes: hazardous, biological, toxic, corrosive, reactive, flammable, radioactive wastes, and construction and demolition...debris resulting from construction contracts. Also excluded are the recycling and composting programs paid by Environmental PEs. 5. Real Property...waxes, toilet tissue, mops, brooms) Accounts for all activities associated with the management and costs for custodial (i.e. carpet cleaning, window

  15. Dredging Operations Technical Support Program. Long-Term Monitoring of Eleven Corps of Engineers Habitat Development Field Sites Built of Dredged Material, 1974-1987

    Science.gov (United States)

    1989-12-01

    Pteridiwn aqui ~inum Broadleaf arrowhead Saittaria latifolia Broadleaf cattail Typha latifolia Brome grass Bromus sp. Broom sedge Andropogon virginicus... grass Eremochloa ophiuroides Cheat grass Bromus tectorwn Chuf a Cyperus esculentus Chinese tallow Sapium sebiferwn Cinquefoil Potentilla spp. Clammy...Dallis grass Paspalum dilatatwn Dandelion Taraxacum officinale Dayf lower Commelina sp. Deer pea Vigna luteola Deertongue grass Panicwn clandestinwn

  16. National Wetland Plant List Indicator Rating Definitions

    Science.gov (United States)

    2012-07-01

    cut grass), Acorus americanus (sweetflag), Carex aquatilis (leafy tussock sedge ), and Toxicodendron vernix (poison sumac). FACW (Facultative Wetland...where water saturates the soils or floods the soil surface at least seasonally. Examples include Carex scoparia (broom sedge ), Aconitum columbianum...Ambrosia artemisifolia (annual ragweed), Betula papyrifera (paper birch), Carex eburnea (bristle-leaf sedge ), Carya ovata (shag-bark hickory), Elymus

  17. Phytoplankton composition and biomass across the southern Indian Ocean

    DEFF Research Database (Denmark)

    Schlüter, Louise; Henriksen, Peter; Nielsen, Torkel Gissel

    2011-01-01

    . The different plankton communities detected reflected the different water masses along a transect from Cape Town, South Africa, to Broome, Australia. The first station was influenced by the Agulhas Current with a very deep mixed surface layer. Based on pigment analysis this station was dominated by haptophytes...

  18. Oceanography and the base of the pelagic food web in the southern Indian Ocean

    DEFF Research Database (Denmark)

    Visser, Andre; Nielsen, Torkel Gissel; Middelboe, Mathias

    2015-01-01

    Cape Town, South Africa, to Broome in north western Australia. Primary production was tightly linked to water column stability and nutrient availability, with high productivity (1144 mg C m22 day21) in the sub-tropical convergence zone, and falling off by an order of magnitude in the sub-tropical gyre...

  19. Association mapping of fruit, seed and disease resistance traits in Theobroma cacao L

    Science.gov (United States)

    An association mapping approach was employed to find markers for color, size, girth and mass of fruits; seed number and butterfat content; and resistance to black pod and witches’ broom diseases in cacao (Theobroma cacao L.). Ninety-five microsatellites (SSRs) and 775 single nucleotide polymorphisms...

  20. Breeding for disease resistance in cacao

    Science.gov (United States)

    Cacao production must increase in order to meet the projected rise in the demand for chocolate. Approximately one-third of global production is lost annually to diseases and insects. Four diseases account for the greatest losses worldwide: black pod, caused by four Phytophthora spp; witches’ broom...

  1. Cacao diseases: A history of old enemies and new encounters

    Science.gov (United States)

    This book reviews the current knowledge of cacao pathogens and their management methods. Topics discussed include the history, biology, and genetic diversity of Moniliophthora (causing witches’ broom and frosty pod rot) and Phytophthora species (causing black pod rot) that cause diseases resulting i...

  2. Postimehe fotograaf võitis konkursi "Eesti foto 2004" / Neeme Korv

    Index Scriptorium Estoniae

    Korv, Neeme, 1974-

    2004-01-01

    Pärnu Uue Kunsti Muuseumi ja fotoajakirja "Cheese" fotokonkursil osales 67 fotograafi. Grand prix' võitis Ove Maidla broomõlitehnikas fotodega, millel kujutatakse maastikke, aega ja inimesi. Järgnesid Kristo Rihm, Anu Vahtra ja Märten Kross. Parim noor fotograaf oli Laura Nõmmik

  3. Proteome Profiling of Paulownia Seedlings Infected with Phytoplasma

    Science.gov (United States)

    Cao, Xibing; Fan, Guoqiang; Dong, Yanpeng; Zhao, Zhenli; Deng, Minjie; Wang, Zhe; Liu, Wenshan

    2017-01-01

    Phytoplasma is an insect-transmitted pathogen that causes witches' broom disease in many plants. Paulownia witches' broom is one of the most destructive diseases threatening Paulownia production. The molecular mechanisms associated with this disease have been investigated by transcriptome sequencing, but changes in protein abundance have not been investigated with isobaric tags for relative and absolute quantitation. Previous results have shown that methyl methane sulfonate (MMS) can help Paulownia seedlings recover from the symptoms of witches' broom and reinstate a healthy morphology. In this study, a transcriptomic-assisted proteomic technique was used to analyze the protein changes in phytoplasma-infected Paulownia tomentosa seedlings, phytoplasma-infected seedlings treated with 20 and 60 mg·L−1 MMS, and healthy seedlings. A total of 2,051 proteins were obtained, 879 of which were found to be differentially abundant in pairwise comparisons between the sample groups. Among the differentially abundant proteins, 43 were related to Paulownia witches' broom disease and many of them were annotated to be involved in photosynthesis, expression of dwarf symptom, energy production, and cell signal pathways. PMID:28344590

  4. Use of quantitative real time PCR for a genome-wide study of AYWB phytoplasma gene expression in plant and insect hosts

    DEFF Research Database (Denmark)

    Makarova, Olga; MacLean, Allyson M.; Hogenhout, Saskia A.;

    2011-01-01

    this technique for reliable gene expression quantification of phytoplasmas on a large scale. In our experimental setup, 242 genes of aster yellows phytoplasma strain witches' broom (AY-WB) were tested for differences in expression in plant and insect host environments, and were shown to be predominantly...

  5. Phytoplasma adapt to the diverse environments of their plant and insect hosts by altering gene expression

    DEFF Research Database (Denmark)

    Makarova, Olga; MacLean, Allyson M.; Nicolaisen, Mogens

    2015-01-01

    Phytoplasmas are intracellular insect-transmitted phytopathogenic bacteria with small genomes. To understand how Aster Yellows phytoplasma strain witches' broom (AY-WB) adapts to their hosts, we performed qRT-PCR analysis of 179 in silico functionally annotated AY-WB genes that are likely to have...

  6. Field Surveys, IOC Valleys. Volume III, Part I. Cultural Resources Survey, Dry Lake Valley, Nevada.

    Science.gov (United States)

    1981-08-01

    Artemisia nova) but also include cliffrose (Cowania mexicana ) and broom snakeweed (Gutierrezia sarothreae) as dominant species. Other species include... CULTURA Ale ~~REOUC SURVEYa AREASczCAvE L CU 11U CUUI 3-2 E-TR-48-III-I 69 was used because it is considered intensive by the Bureau of Land Management and

  7. Field Report : Anna Plains and Roebuck Bay Benthic Invertebrate Mapping 2016

    NARCIS (Netherlands)

    Piersma, Theunis; Pearson, Grant B.; Lavaleye, Marc S. S.; Hickey, Robert; Rogers, Danny; Holthuijsen, Sander; Estrella, Sora-Marin; de Goeij, Petra; Findlay, Naomi; Storey, Andrew W.

    2016-01-01

    This project has been funded by the Department of Parks and Wildlife partnership with BHP Billiton “Eighty Mile Beach and Walyarta Conservation Program”, with in-kind support from NIOZ and Wetland Research & Management This report was produced at the Broome Bird Observatory in late October 2016. Abs

  8. First report of ‘Candidatus Phytoplasma asteris’ subgroup 16SrI-A associated with a disease of potato (Solanum tuberosum) in Lithuania

    Science.gov (United States)

    Symptoms of little leaf, witches’-broom, and abnormally small and deformed potatoes, suggestive of possible phytoplasmal infection, were observed in diseased potato (Solanum tuberosum var. Hannibal arba Kestrel) in the Vilnius region of Lithuania. DNA extracted from symptomatic leaves and shoots we...

  9. Proceedings of the Workshop on Meteorological and Environmental Inputs to Aviation Systems (6th) Held at the University of Tennessee Space Institute on 26-28 October 1982,

    Science.gov (United States)

    1983-04-01

    is very important to know " Wind and emperature MesurementsTransmitted to Central both the temperature and the wind field. Figure Databse - Avilable...enhances the ability of the runway to broomed, sanded, treated with chemicals , etc. effectively drain water. The FAA has resisted the use of striated paint

  10. Field Report: Anna Plains and Roebuck Bay Benthic Invertebrate Mapping 2016 : AnnRoeBIM16

    NARCIS (Netherlands)

    Piersma, Theunis; Pearson, Grant B.; Lavaleye, Marc S. S.; Hickey, Robert; Rogers, Danny; Holthuijsen, Sander; Estrella, Sora-Marin; de Goeij, Petra; Findlay, Naomi; Storey, Andrew W.

    2016-01-01

    This project has been funded by the Department of Parks and Wildlife partnership with BHP Billiton “Eighty Mile Beach and Walyarta Conservation Program”, with in-kind support from NIOZ and Wetland Research & Management This report was produced at the Broome Bird Observatory in late October 2016. Abs

  11. Dicty_cDB: SHJ212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s witches'-broom phy... 34 3.7 AY754825_1( AY754825 |pid:none) Human rotavirus C isolate DhakaC13... 33 4.8 ...... 33 8.2 FJ619589_1( FJ619589 |pid:none) Human rotavirus C strain 9298 VP4 ...

  12. Novel laser contact probe for periodontal treatment

    Science.gov (United States)

    Watanabe, Hisashi; Kataoka, Kenzo; Ishikawa, Isao

    2001-04-01

    Application of the erbium: YAG laser to periodontal treatment has been attempted and preferable results have been reported for calculus removal, vaporization of granulation tissue, periodontal pocket sterilization and so on. However, it has been difficult to reach and treat some conditions involving complex root morphology and furcated rots with conventional probes. The new broom probe was designed and tested to overcome these obstacles. The probe was made of 20 super-fine optical fibers bound into a broom shape. The experiments were carried out to evaluate the destructive power of a single fiber and to examine the morphology of tissue destruction and the accessibility to a bifurcated root of a human tooth using the broom probe. The Er:YAG laser prototype was used. A flat specimen plate was made by cutting the root of a cow tooth and then attached to an electrically operated table and irradiated under various conditions. The specimens were examined with both an optical and scanning electron microscope. The irradiated surfaces were also examined with a roughness meter. An irradiation applied with a single fiber with an energy level of 1 to 1.5 mJ at its tip results in a destruction depth of 3 to 24 micrometers . The optimum conditions for the fibers of this probe was 1.0 mJ at 10 pps and a scanning speed of 100 mm/min. No part of the tooth surface remained un-irradiated after using the broom probe to cover the surface 5 times parallel to the tooth axis and then five times at a 30 degree angle to the previous irradiation at a power of 20 mJ at 10 pps. Also curved and irregular surface were destroyed to a maximum depth of 19 micrometers . In conclusion, these results suggest that the broom probe would be applicable for periodontal laser treatments even if the tooth surface has a complex and irregular shape.

  13. New records of Aphyllophorales (Basidiomycota in the Atlantic Rain Forest in Northeast Brazil Novos registros de Aphyllophorales (Basidiomycota em Mata Atlântica no Nordeste brasileiro

    Directory of Open Access Journals (Sweden)

    Tatiana Baptista Gibertoni

    2004-12-01

    Full Text Available Non-poroid Aphyllophorales (Basidiomycota in areas of the Atlantic Rain Forest in Northeast Brazil are reported. Auriscalpium villipes (Lloyd Snell & E.A. Dick, Climacodon pulcherrimus (Berk. & M.A. Curtis Nikol., Gloeodontia discolor (Berk. & M.A. Curtis Boidin, Irpex lacteus (Fr.: Fr. Fr. and Scytinostroma duriusculum (Berk. & Broome Donk are new records to Northeast Brazil.Aphyllophorales (Basidiomycota não poróides foram registrados em áreas de Mata Atlântica do Nordeste brasileiro. Auriscalpium villipes (Lloyd Snell & E.A. Dick, Climacodon pulcherrimus (Berk. & M.A. Curtis Nikol., Gloeodontia discolor (Berk. & M.A. Curtis Boidin, Irpex lacteus (Fr.: Fr. Fr. e Scytinostroma duriusculum (Berk. & Broome Donk são novas ocorrências para o Nordeste do Brasil.

  14. A quantitative striping removal algorithm for HY- 1 CCD data

    Institute of Scientific and Technical Information of China (English)

    孙凌; 唐军武; 张杰

    2002-01-01

    -- Striping is a common phenomenon in remote sensing imagery acquired by most spaceborne and airborne multispectral sensors. In contrast to the destriping of the images of optoelectronic mechanical scanners, fewer work has been done for push-broom CCD images. Based on the characteristics of HY-1 push-broom multispectral CCD camera and its prelaunch radiometric calibration results, the striping features are analyzed and a quantitative striping removal algorithm is proposed. The algorithm is to obtain the inter-detector equalization curves to reflect the differences between detectors by statistic analysis of the satellite imagery data set, and image stripes arising from the nonuniformity of the detectors of the CCD array can be removed using the estimated equalization curves. The preliminary results show that this method can effectively remove the stripes and preserve the radiometric accuracy of the raw data at the same time.

  15. Language disorders as a window on universal grammar: an abstract theory of agreement for IP, DP, and V-PP.

    Science.gov (United States)

    Roeper, T; Ramos, E; Seymour, H; Abdul-Karim, L

    2001-06-01

    A new concept of Agreement (AGR) has been represented as a Formal Feature that can appear in a wide range of different configurations (Chomsky, 1998). A case study from language disorders supports and extends this abstract concept. The child shows no agreement in Inflectional Phrase me can and Determiner Phrase them eyes. We then extend the notion of AGR to include verb-Prepositional Phrase relations, where the child also systematically avoids certain prepositions (go beach). The analysis is supported by intuitional data from compounds (sweep with broom --> broom-swept). We also define a systematic notion of Possible deficit as a premature fixation of functional items which normally require additional Phi-features. The notion of Maximization of Formal Features then emerges as a significant feature of learnability from both a normal and disordered perspective.

  16. Cacao breeding in Bahia, Brazil - strategies and results

    Directory of Open Access Journals (Sweden)

    Uilson Vanderlei Lopes

    2011-01-01

    Full Text Available Cacao was introduced in Bahia in 1756, becoming later the largest producer state in the country. In order to supportthe planting of cacao in the region, a breeding program was established by CEPEC at the beginning of the 1970s. For a long time,the program consisted in testing new hybrids (full-sibs and releasing a mixture of the best ones to farmers. Lately, particularly afterthe witches´ broom arrival in the region, in 1989, recurrent breeding strategies were implemented, aiming mainly the developmentof clones. From 1993 to 2010, more than 500 progenies, accumulating 30 thousand trees, were developed by crossing many parentswith resistance to witches´ broom, high yield and other traits. In this period, more than 500 clones were put in trials and 39 clonesand 3 hybrids were released to farmers. In this paper the strategies and results achieved by the program are reviewed. Overall theprogram has good interface with pathology and genomic programs.

  17. Novel multi-beam radiometers for accurate ocean surveillance

    DEFF Research Database (Denmark)

    Cappellin, C.; Pontoppidan, K.; Nielsen, P. H.

    2014-01-01

    Novel antenna architectures for real aperture multi-beam radiometers providing high resolution and high sensitivity for accurate sea surface temperature (SST) and ocean vector wind (OVW) measurements are investigated. On the basis of the radiometer requirements set for future SST/OVW missions, co......, conical scanners and push-broom antennas are compared. The comparison will cover reflector optics and focal plane array configuration....

  18. Biological restoration of major transportation facilities domestic demonstration and application project (DDAP): technology development at Sandia National Laboratories.

    Energy Technology Data Exchange (ETDEWEB)

    Ramsey, James L., Jr. (.,; .); Melton, Brad; Finley, Patrick; Brockman, John; Peyton, Chad E.; Tucker, Mark David; Einfeld, Wayne; Griffith, Richard O.; Brown, Gary Stephen; Lucero, Daniel A.; Betty, Rita G.; McKenna, Sean Andrew; Knowlton, Robert G.; Ho, Pauline

    2006-06-01

    The Bio-Restoration of Major Transportation Facilities Domestic Demonstration and Application Program (DDAP) is a designed to accelerate the restoration of transportation nodes following an attack with a biological warfare agent. This report documents the technology development work done at SNL for this DDAP, which include development of the BROOM tool, an investigation of surface sample collection efficiency, and a flow cytometry study of chlorine dioxide effects on Bacillus anthracis spore viability.

  19. Eccentric Connectivity Index of Chemical Trees

    CERN Document Server

    c, Aleksandar Ili\\'

    2011-01-01

    The eccentric connectivity index $\\xi^c$ is a distance--based molecular structure descriptor that was recently used for mathematical modeling of biological activities of diverse nature. We prove that the broom has maximum $\\xi^c$ among trees with a fixed maximum vertex degree, and characterize such trees with minimum $\\xi^c$\\,. In addition, we propose a simple linear algorithm for calculating $\\xi^c$ of trees.

  20. The Limits of Friendship: US Security Cooperation in Central Asia (Walker Paper, Number 9)

    Science.gov (United States)

    2007-10-01

    ward Black, Lt Col Taft Blackburn, Jeannie Borden , Barbara Braese, Col Matt Brand, Lt Col David Brigham, Maj kent Broome, Maj Mark Campbell...collapse of the Soviet Union in December 1991, Pres. George H . W. Bush sought to develop an aid program for the newly independent states in Eurasia...September 1995, 4. 43. Neil MacFarlane, Western Engagement in the Caucasus and Central Asia (London: Royal Institute of International Affairs, 1999

  1. 76 FR 11174 - Airworthiness Directives; Sikorsky Aircraft Corporation Model S-76A, S-76B, and S-76C Helicopters...

    Science.gov (United States)

    2011-03-01

    ...-41A. Remove the safety pin, P/N ASI-700-80. Remove and discard any ball bearings, and clean all... safety pin. Release the cable from the broom closet. Reinstall the cable to the handle with the clevis... (handle assembly), P/N ASI-700-41A. (h) Remove the safety pin, P/N ASI-700-80. (i) Remove and discard...

  2. Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees

    OpenAIRE

    Farveh Ehya; Aboozar Monavarfeshani; Ehsan Mohseni Fard; Laleh Karimi Farsad; Mojtaba Khayam Nekouei; Mohsen Mardi; Ghasem Hosseini Salekdeh

    2013-01-01

    Background Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is associated to the phytoplasma ‘Candidatus Phytoplasma aurantifolia’, is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene ...

  3. Advertising value equivalence: PR’s orphan metric

    OpenAIRE

    Watson, Tom

    2013-01-01

    The academic approach to measurement and evaluation has long favoured social science methodologies (Broom and Dozier, 1990, Michaelson and Stacks, 2011 and Stacks, 2002), but there has been persistent, widespread practice use of advertising value equivalence (AVE) to express the economic and financial value of public relations activity. This paper investigates the evolution of AVE and discusses whether it arose from clippings agencies, press agentry or other influences on public relations, su...

  4. Field Report: Anna Plains and Roebuck Bay Benthic Invertebrate Mapping 2016

    OpenAIRE

    Piersma, Theunis; Pearson, Grant B.; Marc S S Lavaleye; Hickey, Robert; Rogers, Danny; Holthuijsen, Sander; Estrella, Sora-Marin; de Goeij, Petra; Findlay, Naomi; Storey, Andrew W.

    2016-01-01

    This project has been funded by the Department of Parks and Wildlife partnership with BHP Billiton “Eighty Mile Beach and Walyarta Conservation Program”, with in-kind support from NIOZ and Wetland Research & Management This report was produced at the Broome Bird Observatory in late October 2016. Abstract 1. This is a report on repeat surveys on the state of the benthic invertebrates at two internationally important areas of intertidal mudflats in northwest Australia (Roebuck Bay and Eighty Mi...

  5. Global Stratotype Section and Point for base of the Ordovician System

    Institute of Scientific and Technical Information of China (English)

    R. A. Cooper; G. S. Nowlan; S. H. Williams

    2001-01-01

    @@ The Global Stratotype Section and Point for base of the Ordovician System and base of the lowest Ordovician stage (Tremadocian) is exposed in the coastal platform at Green Point, western Newfoundland, at the 101.8 m level, within Bed 23, in the measured section (Lower Broom Point Member, Green Point Formation), coinciding with the first appearance of the conodont Iapetognathus fluctivagus Nicoll, Miller, Nowlan,Repetski and Ethington, 1999, and 4.8 m below the earliest planktic graptolites.

  6. Handbook on Bird Management and Control.

    Science.gov (United States)

    1980-03-01

    shrubs, and vines , including ornamental plantings, may attract birds such as swallows, warblers, or Starlings. This food source can be eliminated...Crows, Starlings, and blackbirds that roost in trees can often be discouraged from using the roosting site by topping or thinning (i.e., pruning ) the...required to start the fire, while brush rakes, fire brooms, and backpack pumps are needed to control it. Intensive pruning discourages birds from roosting

  7. The Impact of Organizational Stress and Burnout on Client Engagement

    OpenAIRE

    Landrum, Brittany; Knight, Danica K.; Flynn, Patrick M.

    2011-01-01

    This paper explores the impact of organizational attributes on client engagement within substance abuse treatment. Previous research has identified organizational features, including small size, accreditation, and workplace practices that impact client engagement (Broome, Flynn, Knight, & Simpson, 2007). The current study sought to explore how aspects of the work environment impact client engagement. The sample included 89 programs located in 9 states across the U.S. Work environment measures...

  8. Démocratie d’en-bas ou « démocratie de la rue » ?

    DEFF Research Database (Denmark)

    Bojsen, Heidi; Boni, Seydou

    2017-01-01

    Græsrods- og folkebevægelsen Cititizen’s Broom, der blev grundlagt i 2013 er blevet langt mere populær og opnået langt større indlydelse end nogen havde kunnet forudse da dens første aktiviteter fandt sted. Som følge af opstanden i okotober 2014 i Burkina Faso og i overgangsregimet, der fulgte, e...

  9. Soil, water and nutrient conservation in mountain farming systems: case-study from the Sikkim Himalaya.

    Science.gov (United States)

    Sharma, E; Rai, S C; Sharma, R

    2001-02-01

    The Khanikhola watershed in Sikkim is agrarian with about 50% area under rain-fed agriculture representing the conditions of the middle mountains all over the Himalaya. The study was conducted to assess overland flow, soil loss and subsequent nutrient losses from different land uses in the watershed, and identify biotechnological inputs for management of mountain farming systems. Overland flow, soil and nutrient losses were very high from open agricultural (cropped) fields compared to other land uses, and more than 72% of nutrient losses were attributable to agriculture land use. Forests and large cardamom agroforestry conserved more soil compared to other land uses. Interventions, like cultivation of broom grass upon terrace risers, N2-fixing Albizia trees for maintenance of soil fertility and plantation of horticulture trees, have reduced the soil loss (by 22%). Soil and water conservation values (> 80%) of both large cardamom and broom grass were higher compared to other crops. Use of N2-fixing Albizia tree in large cardamom agroforestry and croplands contributed to soil fertility, and increased productivity and yield. Bio-composting of farm resources ensured increase in nutrient availability specially phosphorus in cropped areas. Agricultural practices in mountain areas should be strengthened with more agroforestry components, and cash crops like large cardamom and broom grass in agroforestry provide high economic return and are hydroecologically sustainable.

  10. An Optimal Beamforming Algorithm for Phased-Array Antennas Used in Multi-Beam Spaceborne Radiometers

    DEFF Research Database (Denmark)

    Iupikov, O. A.; Ivashina, M. V.; Pontoppidan, K.

    2015-01-01

    Strict requirements for future spaceborne ocean missions using multi-beam radiometers call for new antenna technologies, such as digital beamforming phased arrays. In this paper, we present an optimal beamforming algorithm for phased-array antenna systems designed to operate as focal plane arrays...... (FPA) in push-broom radiometers. This algorithm is formulated as an optimization procedure that maximizes the beam efficiency, while minimizing the side-lobe and cross-polarization power in the area of Earth, subject to a constraint on the beamformer dynamic range. The proposed algorithm is applied...

  11. Pruning high-value Douglas-fir can reduce dwarf mistletoe severity and increase longevity in central Oregon

    Science.gov (United States)

    Maffei, Helen M; Filip, Gregory M; Gruelke, Nancy E; Oblinger, Brent W; Margolis, Ellis; Chadwick, Kristen L

    2016-01-01

    Mid- to very large-sized Douglas-fir (Pseudotsuga menzieseii var. menziesii) that were lightly- to moderately-infected by dwarf mistletoe (Arceuthobium douglasii) were analyzed over a 14-year period to evaluate whether mechanical pruning could eradicate mistletoe (or at least delay the onset of severe infection) without significantly affecting tree vitality and by inference, longevity. Immediate and longterm pruning effects on mistletoe infection severity were assessed by comparing pruned trees (n = 173) to unpruned trees (n = 55) with respect to: (1) percentage of trees with no visible infections 14 years post-pruning, (2) Broom Volume Rating (BVR), and (3) rate of BVR increase 14 years postpruning. Vitality/longevity (compared with unpruned trees) was assessed using six indicators: (1) tree survival, (2) the development of severe infections, (3) the development of dead tops, (4) tree-ring width indices, (5) Normalized Difference Vegetation Index (NDVI) from high-resolution multi-spectral imagery, and (6) live-crown ratio (LCR) and increment. Twenty-four percent of the pruned trees remained free of mistletoe 14 years post-pruning. Pruning is most likely to successfully eradicate mistletoe in lightly infected trees (BVR 1 or 2) without infected neighbors. Pruning significantly decreased mean BVR in the pruned versus the unpruned trees. However, the subsequent average rate of intensification (1.3–1.5 BVR per decade) was not affected, implying that a single pruning provides ~14 years respite in the progression of infection levels. Post-pruning infection intensification was slower on dominant and co-dominants than on intermediate or suppressed trees. The success of mistletoe eradication via pruning and need for follow-up pruning should be evaluated no sooner than 14 years after pruning to allow for the development of detectable brooms. Based on six indicators, foliage from witches brooms contribute little to long-term tree vitality since removal appears to have

  12. Inverse Eigenvalue Problems for Two Special Acyclic Matrices

    Directory of Open Access Journals (Sweden)

    Debashish Sharma

    2016-03-01

    Full Text Available In this paper, we study two inverse eigenvalue problems (IEPs of constructing two special acyclic matrices. The first problem involves the reconstruction of matrices whose graph is a path, from given information on one eigenvector of the required matrix and one eigenvalue of each of its leading principal submatrices. The second problem involves reconstruction of matrices whose graph is a broom, the eigen data being the maximum and minimum eigenvalues of each of the leading principal submatrices of the required matrix. In order to solve the problems, we use the recurrence relations among leading principal minors and the property of simplicity of the extremal eigenvalues of acyclic matrices.

  13. A General Epipolar-Line Model between Optical and SAR Images and Used in Image Matching

    Directory of Open Access Journals (Sweden)

    Shuai Xing

    2014-02-01

    Full Text Available The search space and strategy are important for optical and SAR image matching. In this paper a general epipolar-line model has been proposed between linear array push-broom optical and SAR images. Then a dynamic approximate epipolar-line constraint model (DAELCM has been constructed and used to construct a new image matching algorithm with Harris operator and CRA. Experimental results have shown that the general epipolar-line model is valid and successfully used in optical and SAR image matching, and effectively limits the search space and decreased computation.

  14. 中国的球盖菇科(五) 广义斑褶菇属%Strophariaceae of China (Ⅴ) Panaeolus s. L.

    Institute of Scientific and Technical Information of China (English)

    图力古尔; 萨仁图雅

    2010-01-01

    通过观察吉林农业大学菌物研究所标本馆 (HMJAU)、中国科学院微生物研究所菌物标本馆 (HMAS)、广东微生物研究所标本馆 (GDGM)及作者野外采集的共180份标本的宏观形态和微观结构,对中国球盖菇科的广义斑褶菇属及其所含的灰斑褶菇属 (Copelandia Bres.)、疣孢斑褶菇属 (Panaeolina Maire)和狭义斑褶菇属 [Panaeolus (Fr.) Quél(s. str.)]进行了分类学研究,对中国分布的16个种进行了形态学描述、显微线条图绘制,并编写了分种检索表.其中,包括1个新组合:栗褶疣孢斑褶菇[Panaeolina castaneifolia (Murr.) Sarentoya et Tolgor];2个中国新记录种:热带灰斑褶菇[Copelandia tropicalis (Ola′h) Singer & R.A. Weeks]、小型斑褶菇[Panaeolus alcidis M.M. Moser];12个省级新记录种:北京新记录种硬腿斑褶菇 [Panaeolus solidipes (Peck) Sacc.],内蒙古新记录种硬腿斑褶菇 [Panaeolus solidipes (Peck) Sacc.]、锐顶斑褶菇[Panaeolus acuminatus (Schaeff.) Quél.]、黑斑褶菇[Panaeolus ater (J.E. Lange) Kühner & Romagn.]、紧缩斑褶菇[Panaeolus sphinctrinus (Fr.) Quél.]、红褐斑褶菇[Panaeolus subbalteatus (Berk. & Broome) Sacc.],吉林省新记录种半卵圆斑褶菇 [Panaeolus semiovatus(Sowerby) S. Lundell & Nannf.]、栗褶疣孢斑褶菇[Panaeolina castaneifolia (Murrill) Bon],黑龙江新记录种红褐斑褶菇[Panaeolus subbalteatus (Berk. & Broome) Sacc.],宁夏新记录种变蓝灰斑褶菇 [Copelandia cyanescens (Berk. & Broome) Singer]、锐顶斑褶菇[Panaeolus acuminatus (Schaeff.) Quél.],西藏新记录种红褐斑褶菇[Panaeolus subbalteatus (Berk. & Broome) Sacc.].

  15. STRUCTURE CONTROL IN HYDROXYAPATITE SYNTHESIS BY HYDROTHERMAL REACTION AND ORGANIC MODULATORS

    Institute of Scientific and Technical Information of China (English)

    Huigang Zhang; Qingshan Zhu

    2005-01-01

    Properties of materials are determined not only by their composition, but also by their structure. It is,therefore, of great significance to develop the ability of tailoring the structure of materials according to the requirements of intended applications. In this work a novel glutamic acid-assisted hydrothermal process was developed to synthesize apatite crystals with controllable morphologies. Varied morphologies from whisk broom, fibers, mushroom, prickly spheres, to dandelion were produced through modulating both the formation of a transitory phase and its transformation into the final phase.

  16. On RPC Model of Satellite Imagery

    Institute of Scientific and Technical Information of China (English)

    ZHANG Guo; YUAN Xiuxiao

    2006-01-01

    The RPC model has recently raised considerable interest in the photogrammetry and remote sensing community. The RPC is a generalized sensor model that is capable of achieving high approximation accuracy. Unfortunately, the computation of the parameters of RPC model is subject to the initial of the parameter in all available literature. An algorithm for computation of parameters of RPC model without initial value is presented and tested on SPOT-5, CBERS-2, ERS-1 imageries. RPC model is suitable for both push-broom and SAR imagery.

  17. Thin, applied surfacing for improving skid resistance of concrete pavements

    Science.gov (United States)

    Scholer, C. F.

    1980-12-01

    The use of select aggregate in a thin wearing surface of portland cement mortar to prolone or restore a concrete pavement's ability to develop high friction was accomplished. Two fine aggregates, blast furnace slag and lightweight expanded shale were found to exhibit skid resistance greater than the other aggregates evaluated. The British polishing wheel was used in the laboratory evaluation of aggregate to simulate wear. The need for a method of restoring friction to a worn, but otherwise sound concrete pavement led to a field evaluation of several different techniques for placing a very thin overlay. The successful method was a broomed, very thin layer of mortar, 3 mm thick.

  18. Correlation of metabolic changes of infected paulownia tissue culture with PWB-MLO pathogenic mechanism

    Institute of Scientific and Technical Information of China (English)

    田国忠; 黄钦才; 袁巧平; 张锡津

    1995-01-01

    Paulownia sp.tissue culture plantlets infected with paulownia witches’ broom mycoplasma-likeorganism (PWB-MLO) expressed a typical witches’ broom symptom.The results determined by transmissionelectron microscopy and DAPI staining fluorescence microscopy showed that the severity of the symptom wasdirectly related with MLO concentration in the sieve tubes of the phloem.The results of the analysis ofendogenous hormones and phenolic compounds by HPLC revealed that in all cases,the free IAA content ofthe diseased plant apparently lower than that of the healthy,and the free IAA concentration had strikinglypositive correlation with catechol concentration changes (r=0.90,n= 11).The changes of cytokinin (Z +ZR,iPA),GA3 and ABA were shown less relation with MLO infection.The differences of peroxidase andisoenzyme,IAA oxidase and phenylanine ammonia lyase (PAL) between the extract of infected plants andthat of uninfected as well as different effects of NAA and catechol additions on MS media could further re-flect the nature of the relationship and the interaction among MLOs,catechol and the free IAA concentration.

  19. Phytoplasma-induced floral abnormalities in Catharanthus roseus are associated with phytoplasma accumulation and transcript repression of floral organ identity genes.

    Science.gov (United States)

    Su, Yi-Ting; Chen, Jen-Chih; Lin, Chan-Pin

    2011-12-01

    Floral symptoms caused by phytoplasma largely resemble floral reversion in other plants. Periwinkle leaf yellowing (PLY) phytoplasma and peanut witches'-broom (PnWB) phytoplasma caused different degrees of floral abnormalities on infected periwinkle plants. The PLY phytoplasma-infected plants exhibited floral discoloration, virescence, small flowers, and only occasionally full floral reversion. In contrast, PnWB phytoplasma frequently induced complete floral reversion and resulted in a witches'-broom symptom from the floral reversion. Although different degrees of floral symptoms were induced by these two phytoplasmas, the morphological disorders were similar to those of other plants carrying SEPALLATA mutations or gene silencing. Here, we compared expression levels of organ-identity-related genes and pigmentation genes during floral symptom development. Accumulation of phytoplasmas in malformed flowers and their closely surrounding leaves was also compared. In infected plants, transcript abundance of all examined organ identity genes and pigmentation genes was suppressed. Indeed, CrSEP3, a SEPALLALA3 ortholog, showed the greatest suppression among genes examined. Of the pigmentation genes, transcript reduction of chalcone synthase was most highly correlated with the loss in floral pigmentation. Floral symptom severities were associated with the accumulation of either phytoplasmas. Interestingly, both phytoplasmas accumulated to higher levels in malformed flowers than in their surrounding leaves. Many plant pathogens manipulate host plant development to their advantage. It is intriguing to see whether phytoplasmas alter floral development to increase their population.

  20. A Two-Phase Space Resection Model for Accurate Topographic Reconstruction from Lunar Imagery with PushbroomScanners

    Directory of Open Access Journals (Sweden)

    Xuemiao Xu

    2016-04-01

    Full Text Available Exterior orientation parameters’ (EOP estimation using space resection plays an important role in topographic reconstruction for push broom scanners. However, existing models of space resection are highly sensitive to errors in data. Unfortunately, for lunar imagery, the altitude data at the ground control points (GCPs for space resection are error-prone. Thus, existing models fail to produce reliable EOPs. Motivated by a finding that for push broom scanners, angular rotations of EOPs can be estimated independent of the altitude data and only involving the geographic data at the GCPs, which are already provided, hence, we divide the modeling of space resection into two phases. Firstly, we estimate the angular rotations based on the reliable geographic data using our proposed mathematical model. Then, with the accurate angular rotations, the collinear equations for space resection are simplified into a linear problem, and the global optimal solution for the spatial position of EOPs can always be achieved. Moreover, a certainty term is integrated to penalize the unreliable altitude data for increasing the error tolerance. Experimental results evidence that our model can obtain more accurate EOPs and topographic maps not only for the simulated data, but also for the real data from Chang’E-1, compared to the existing space resection model.

  1. Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).

    Science.gov (United States)

    Lopes, M A; Hora, B T; Dias, C V; Santos, G C; Gramacho, K P; Cascardo, J C M; Gesteira, A S; Micheli, F

    2010-07-06

    Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches' broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course--from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches' broom resistance, facilitating the breeding and selection of resistant cacao trees.

  2. Shotgun proteomic analysis of the Mexican lime tree infected with "CandidatusPhytoplasma aurantifolia".

    Science.gov (United States)

    Monavarfeshani, Aboozar; Mirzaei, Mehdi; Sarhadi, Elham; Amirkhani, Ardeshir; Khayam Nekouei, Mojtaba; Haynes, Paul A; Mardi, Mohsen; Salekdeh, Ghasem Hosseini

    2013-02-01

    Infection of Mexican lime trees (Citrus aurantifolia L.) with the specialized bacterium "CandidatusPhytoplasma aurantifolia" causes witches' broom disease. Witches' broom disease has the potential to cause significant economic losses throughout western Asia and North Africa. We used label-free quantitative shotgun proteomics to study changes in the proteome of Mexican lime trees in response to infection by "Ca. Phytoplasma aurantifolia". Of 990 proteins present in five replicates of healthy and infected plants, the abundances of 448 proteins changed significantly in response to phytoplasma infection. Of these, 274 proteins were less abundant in infected plants than in healthy plants, and 174 proteins were more abundant in infected plants than in healthy plants. These 448 proteins were involved in stress response, metabolism, growth and development, signal transduction, photosynthesis, cell cycle, and cell wall organization. Our results suggest that proteomic changes in response to infection by phytoplasmas might support phytoplasma nutrition by promoting alterations in the host's sugar metabolism, cell wall biosynthesis, and expression of defense-related proteins. Regulation of defense-related pathways suggests that defense compounds are induced in interactions with susceptible as well as resistant hosts, with the main differences between the two interactions being the speed and intensity of the response.

  3. Transformation of TCS Gene to Paulownia elongata and Its Virus Resistance%TCS基因转化泡桐及抗病能力

    Institute of Scientific and Technical Information of China (English)

    刘静; 黄艳艳; 翁曼丽; 罗磊; 张虹; 王长宪; 牛庆霖; 冯殿齐

    2011-01-01

    @@ 泡桐(Paulownia)是经济价值较高的优良绿化造林树种.由于丛枝病的危害,每年我国林业的直接经济损失就高达数千万元,并且严重影响农民种植泡桐的积极性(杨俊秀等,2007).%In this study, the trichosanthin (TCS) gene, which was cloned from Trichosanthes kirilowii, was transformed into Paulownia elongata through agrobacterium-mediated method. The conversion rate was 29.2% with 86 transformed strains obtained. The target gene had approximately 50% positive expression rate in plantlets through 11 times subculture and PCR detection for 4 times. And the PCR detection of the plants in field showed that the positive rate was between 40% and 50%. No positive expression of the labeled product of witches' broom was found in the transformed plants after infection susceptible experiment and molecular detection, meanwhile, the virus infection rate of the control was up to 15.0%. Thus, it indicated that, compared to the control, the transformed plants showed higher resistance to the witches'broom disease.

  4. Plant-Pathogen Interaction, Circadian Rhythm, and Hormone-Related Gene Expression Provide Indicators of Phytoplasma Infection in Paulownia fortunei

    Directory of Open Access Journals (Sweden)

    Guoqiang Fan

    2014-12-01

    Full Text Available Phytoplasmas are mycoplasma-like pathogens of witches’ broom disease, and are responsible for serious yield losses of Paulownia trees worldwide. The molecular mechanisms of disease development in Paulownia are of considerable interest, but still poorly understood. Here, we have applied transcriptome sequencing technology and a de novo assembly approach to analyze gene expression profiles in Paulownia fortunei infected by phytoplasmas. Our previous researches suggested that methyl methane sulfonated (MMS could reverse the effects of the infection. In this study, leaf samples from healthy, infected, and both infected and methyl methane sulfonate treated plants were analyzed. The results showed that the gene expression profile of P. fortunei underwent dramatic changes after Paulownia witches’ broom (PaWB phytoplasma infection. Genes that encoded key enzymes in plant-pathogen interaction processes were significantly up-regulated in the PaWB-infected Paulownia. Genes involved in circadian rhythm and hormone-related genes were also altered in Paulownia after PaWB infection. However, after the PaWB-infected plants were treated with MMS, the expression profiles of these genes returned to the levels in the healthy controls. The data will help identify potential PaWB disease-resistance genes that could be targeted to inhibit the growth and reproduction of the pathogen and to increase plant resistance.

  5. Plant-pathogen interaction, circadian rhythm, and hormone-related gene expression provide indicators of phytoplasma infection in Paulownia fortunei.

    Science.gov (United States)

    Fan, Guoqiang; Dong, Yanpeng; Deng, Minjie; Zhao, Zhenli; Niu, Suyan; Xu, Enkai

    2014-12-12

    Phytoplasmas are mycoplasma-like pathogens of witches' broom disease, and are responsible for serious yield losses of Paulownia trees worldwide. The molecular mechanisms of disease development in Paulownia are of considerable interest, but still poorly understood. Here, we have applied transcriptome sequencing technology and a de novo assembly approach to analyze gene expression profiles in Paulownia fortunei infected by phytoplasmas. Our previous researches suggested that methyl methane sulfonated (MMS) could reverse the effects of the infection. In this study, leaf samples from healthy, infected, and both infected and methyl methane sulfonate treated plants were analyzed. The results showed that the gene expression profile of P. fortunei underwent dramatic changes after Paulownia witches' broom (PaWB) phytoplasma infection. Genes that encoded key enzymes in plant-pathogen interaction processes were significantly up-regulated in the PaWB-infected Paulownia. Genes involved in circadian rhythm and hormone-related genes were also altered in Paulownia after PaWB infection. However, after the PaWB-infected plants were treated with MMS, the expression profiles of these genes returned to the levels in the healthy controls. The data will help identify potential PaWB disease-resistance genes that could be targeted to inhibit the growth and reproduction of the pathogen and to increase plant resistance.

  6. Effect of 7 botany species of plants on Zabrotes subfasciatus (Bohemann

    Directory of Open Access Journals (Sweden)

    Roberto Valdés Herrera

    2016-03-01

    Full Text Available The effect of several plant in the control of weevil Zabrotes subfasciatus (Boh. (Coleoptera; Bruchidae was evaluated. The plant were caisimón of anisette, apasote, bitter broom, sage, sassafras, garlic leek and nim were used. In the experiment they were carried out 2 variants, one to see the effect on a female of the insect and another to observe the behavior in group of Z. subfasciatus. The effects of the same ones were evaluated in the ovoposición and in the time of permanency of the insect on each plant residual. the death to Z. subfasciatus. The residuals of the molinaje of bitter broom, I age leek, caisimón of anisette and sage those that bigger repelencia effect had were, as much in the permanency as in the number of eggs ovopositados for the female of Z. subfasciatus. Correlation didn’t exist among the time of permanency of the insect in the different treatments and the number of on eggs. The biggest quantity in eggs was ovopositados it has more than enough sassafras.

  7. Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

    Directory of Open Access Journals (Sweden)

    Carolina S. Benjamin

    2016-07-01

    Full Text Available The known sources of resistance to witches’ broom (WB, a severe disease of cacao, are limited. Aiming to identify families and parents resistant to Moniliophthora perniciosa, a population of 22 families was evaluated by assessing the number of brooms formed per tree during 10 years under field conditions. The population was established in randomized blocks with three replications of 12 plants each. Significant differences were observed among families. The most outstanding families were NA33 x RB39 and RB39 x P4B, which presented the lowest WB incidence during 10 years. The increase in natural field infection of Scavina clones families and their descendants were clearly demonstrated after 2006. The existence of additive effects for resistance appears clearly for families, which had other source of resistance associated with Scavina. Clones RB39, RB36, P4B, NA33 and CSUL3 are promising parents for pyramiding resistance genes and increasing the stability and durability of resistance to WB.

  8. A qualitative phytochemical analysis and a comparative study of the antibactrial activity of Retama stalks (raetam

    Directory of Open Access Journals (Sweden)

    Hamza Bensaci

    2016-03-01

    Full Text Available The present work is aimed mainly to investigate and compare the antibacterial activities of methanolic, diethyl ether and ethyl acetate extracts of retama on Escherichia coli, Salmonella, Proteus mirabilis and Staphylococcus aureu using well difusion method.The results of study showed a significant effect on all bacterial species except Proteus mirabilis. The preliminary test of retama constituents revealed the presence of active material : Resins, Volatil oils, Coumarins, Terpenes, Phenols, Tannins, Alkaloids, Saponins, Cardiac glycosides, and Flavons. The highest Inhibition rate of Salmonella is 16 mm at the concentration 100 mg/ml, while the lowest inhibition rate was 8 mm for Escherichia coli at concentration 1 mg/ ml in methanolic extract. The results obtained in the present study suggest that the retama stalks (broom broom can be used in treating deseases caused by the tested organisms. Further chemical and pharmacological investigations may be carried out to isolate and identify the chemical constituents in the selected plants responsible for the antimicrobial activity.

  9. Sources of resistance to Crinipellis perniciosa in progenies of cacao accessions collected in the Brazilian Amazon Fontes de resitência a Crinipellis perniciosa em progênies de cacaueiros coletados na Amazônia brasileira

    Directory of Open Access Journals (Sweden)

    Valéria Rodrigues Lavigne de Mello Paim

    2006-12-01

    Full Text Available The witches' broom disease caused by the fungus Crinipellis perniciosa is the main phytossanitary constraint for cacao production in Brazil. The integrated management of the disease involves resistance as one of the components. The breeding program conducted by the Brazilian Institution, CEPLAC is directed toward the pyramidation of resistance genes from different sources to achieve a more durable resistance. This study aimed to identify sources of resistance in progenies of cacao accessions collected in the basins of ten Amazonian rivers and compared to progenies from the Peruvian clones 'Scavina 6' and 'Sacavina 12'. Progenies from 40 Amazonian accessions and 'Scavina' were evaluated in the field for six years for witches' broom resistance through multivariate and repeated measurement analyses evaluating the effect of progeny, area, block, year, and their interactions. There were differences in the mean number of vegetative brooms on some Amazonian progenies and 'Scavina' descendants. There was an increase in the number of vegetative brooms in the last year for 'Scavina' progenies, but that was not observed for the Amazonian progenies 64, 66, 156, 194, 195, 269 and 274. There were different gene/alleles for resistance in the Amazonian progenies in comparison to the traditional 'Scavina' accessions. These new sources of resistance will be important for pyramiding resistance genes and consequently increasing the stability and durability of the resistance to witches' broom.A doença vassoura-de-bruxa, causada pelo fungo Crinipellis perniciosa, é o principal problema fitossanitário para o cultivo do cacaueiro no Brasil. O manejo integrado da doença envolve a resistência como um dos componentes. O programa de melhoramento genético do cacaueiro conduzido pela Instituição brasileira CEPLAC é direcionado para acumular genes de resistência de diferentes fontes visando à obtenção de uma resistência mais durável. O objetivo deste estudo foi

  10. Automated Ortho-Rectification of UAV-Based Hyperspectral Data over an Agricultural Field Using Frame RGB Imagery

    Directory of Open Access Journals (Sweden)

    Ayman Habib

    2016-09-01

    Full Text Available Low-cost Unmanned Airborne Vehicles (UAVs equipped with consumer-grade imaging systems have emerged as a potential remote sensing platform that could satisfy the needs of a wide range of civilian applications. Among these applications, UAV-based agricultural mapping and monitoring have attracted significant attention from both the research and professional communities. The interest in UAV-based remote sensing for agricultural management is motivated by the need to maximize crop yield. Remote sensing-based crop yield prediction and estimation are primarily based on imaging systems with different spectral coverage and resolution (e.g., RGB and hyperspectral imaging systems. Due to the data volume, RGB imaging is based on frame cameras, while hyperspectral sensors are primarily push-broom scanners. To cope with the limited endurance and payload constraints of low-cost UAVs, the agricultural research and professional communities have to rely on consumer-grade and light-weight sensors. However, the geometric fidelity of derived information from push-broom hyperspectral scanners is quite sensitive to the available position and orientation established through a direct geo-referencing unit onboard the imaging platform (i.e., an integrated Global Navigation Satellite System (GNSS and Inertial Navigation System (INS. This paper presents an automated framework for the integration of frame RGB images, push-broom hyperspectral scanner data and consumer-grade GNSS/INS navigation data for accurate geometric rectification of the hyperspectral scenes. The approach relies on utilizing the navigation data, together with a modified Speeded-Up Robust Feature (SURF detector and descriptor, for automating the identification of conjugate features in the RGB and hyperspectral imagery. The SURF modification takes into consideration the available direct geo-referencing information to improve the reliability of the matching procedure in the presence of repetitive texture

  11. Infrared detectors for space applications

    Science.gov (United States)

    Fick, Wolfgang; Gassmann, Kai Uwe; Haas, Luis-Dieter; Haiml, Markus; Hanna, Stefan; Hübner, Dominique; Höhnemann, Holger; Nothaft, Hans-Peter; Thöt, Richard

    2013-12-01

    The motivation and intended benefits for the use of infrared (IR) detectors for space applications are highlighted. The actual status of state-of-the-art IR detectors for space applications is presented based on some of AIM's currently ongoing focal plane detector module developments covering the spectral range from the short-wavelength IR (SWIR) to the long-wavelength IR (LWIR) and very long-wavelength IR (VLWIR), where both imaging and spectroscopy applications will be addressed. In particular, the integrated detector cooler assemblies for a mid-wavelength IR (MWIR) push-broom imaging satellite mission, for the German hyperspectral satellite mission EnMAP and the IR detectors for the Sentinel 3 SLSTR will be elaborated. Additionally, dedicated detector modules for LWIR/VLWIR sounding, providing the possibility to have two different PVs driven by one ROIC, will be addressed.

  12. EKSPLORASI SPASIAL CENDAWAN TANAH PADA SEKITAR RHIZOSFER TANAMAN JAMBU METE (Anacardium occidentale L. DI KARANGASEM DAN BULELENG-BALI

    Directory of Open Access Journals (Sweden)

    Farah Meita Pratiwi

    2013-12-01

    Full Text Available Coconut is one of an important plant to fulfil human’s life needed. This study wasconducted to determine the benefits of coconut plant parts. The study was conducted in someareas of Denpasar and Badung from 27 January until 4 February, 2012. The method used in thisstudy is exploratory survey method by using a questionnaire and interviews. The results showedthat the mostly used parts of the plant are fruit part of 53% (35% bungkak water, bungkak meat12.9%, 4.7% shell root 2.3%, 22 % stems, and leaves as many as 23%. The coconut tree is usedas an upakara (31%, drugs (24%, construction (14%, consumer (13%, crafts (2%, fuel (8%,roofs (2%, broom (2%, and household items (4%.Keywords: Cocos nucifera L. etnobotany, exploration survey

  13. An imaging spectro-polarimeter for measuring hemispherical spectrally resolved down-welling sky polarization

    Science.gov (United States)

    Chenault, David B.; Pezzaniti, J. L.; Roche, Michael; Hyatt, Brian

    2016-05-01

    A full sky imaging spectro-polarimeter has been developed that measures spectrally resolved (~2.5 nm resolution) radiance and polarization (𝑠0, 𝑠1, 𝑠2 Stokes Elements) of natural sky down-welling over approximately 2π sr between 400nm and 1000nm. The sensor is based on a scanning push broom hyperspectral imager configured with a continuously rotating polarizer (sequential measurement in time polarimeter). Sensor control and processing software (based on Polaris Sensor Technologies Grave' camera control software) has a straight-forward and intuitive user interface that provides real-time updated sky down-welling spectral radiance/polarization maps and statistical analysis tools.

  14. Effectiveness of the multidimensional ergonomic intervention model to reduce musculoskeletal discomfort among street sweepers in Chiang Rai Province, Thailand.

    Science.gov (United States)

    Pintakham, Kanjanar; Siriwong, Wattasit

    2016-01-01

    The aim of this study was to evaluate the effectiveness of multidimensional ergonomic intervention (MEI) model to reduce musculoskeletal discomfort (MSD) among street sweepers. A quasi-experimental study was conducted in Chiang Rai Province. The MEI model was designed on the basis of four core components: cognitive behavior therapy, ergonomic education training, stretching exercise, and the foam sleeve broom handle grip. Seventy-five street sweepers volunteered for the screening process on MSD of having level score ≥4 by physiotherapist. Face to face interviews were used mainly in order to diagnose MSD. Physical examination was performed by physiotherapist and physical performance by sports scientist. The findings showed that the MEI model among the intervention group significantly reduced MSD compared with that among control group at exit model and follow-up (P<0.01). This research suggests that the MEI model was appropriate to reduce MSD associated with repetitive movement and awkward postures on task.

  15. Study of root and leaf rachis of Spathelia excelsa: phytochemistry and activity against fungus Moniliophthora perniciosa associated with cupuassu (Theobroma grandiflorum); Estudo em raiz e raquis foliar de Spathelia excelsa: fitoquimica e atividade frente ao fungo Moniliophthora perniciosa associado ao cupuacuzeiro (Theobroma grandiflorum)

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Loretta Ennes de; Lima, Maria da Paz; Maximo, Ariane da Costa; Pereira, Elaine Cristina da Silva; Moreira, Wagner Alan dos Santos [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Tecnologia e Inovacao; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Veras, Solange de Mello [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Fac. de Ciencias Agrarias; Souza, Maria Geralda de, E-mail: mdapaz@inpa.gov.br [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Manaus, AM (Brazil)

    2012-07-01

    The chemical composition of Spathelia excelsa (Krause) R. S. Cowan and Brizicky was investigated and the limonoids harrisonin (1) and deacetylspathelin (2), alkaloids folinin and casimiroin mixture (3a,b), plus a further casimiroin (3b) were identified in methanol extract from root. The CH{sub 2}Cl{sub 2} extract from the rachis yielded protolimonoid 3{beta}-angeloyl-21,24-epoxy-7{alpha},21{alpha},23{alpha},25-tetrahydroxy-4{alpha},4{beta},8{beta},10{beta}-tetramethyl -25-dimethyl-14,18-cyclo-5{alpha},13{alpha},14{alpha},17{alpha}-cholestane (4), and methanol extract, the limonoids limonin diosphenol (5) and perforatin (6), as well as the chromone biflorin (7). Harrisonin and biflorin were isolated for the first time in this genus. On the antifungal assay against witches' broom (Moniliophthora perniciosa) compound 3b was found to be active. (author)

  16. Preliminary study on mercury uptake by Rosmarinus officinalis L. (Rosemary) in a mining area (Mt. Amiata, Italy)

    Energy Technology Data Exchange (ETDEWEB)

    Barghigiani, C.; Ristori, T. [Institute of Biophysics, Pisa (Italy)

    1995-04-01

    Among the different plants analyzed to assess environmental mercury contamination of mining areas, lichens are those most studied, followed by brooms together with pine, which was also used in other areas, and spruce. Other species, both naturally occurring and cultivated, have also been studied. This work reports on the results of mercury uptake and accumulation in rosemary in relation to metal concentrations in both air and soil. R. officinalis is a widespread endemic Mediterranean evergreen shrub, which in Italy grows naturally and is also cultivated as a culinary herb. This research was carried out in Tuscany (Italy), in the Mt. Amiata area, which is characterized by the presence of cinnabar (HgS) deposits and has been used for mercury extraction and smelting from Etruscan times until 1980, and in the country near the town of Pisa, 140 km away from Mt. Amiata. 16 refs., 3 figs., 1 tab.

  17. An automatic de-striping algorithm for Ocean Colour Monitor Imagery

    Science.gov (United States)

    Tripathi, Santosh

    2012-07-01

    OCM-2 Mission is envisaged as the continuity service provider to Oceansat-1 (IRS-P4) with enhanced application potential. Oceansat-2 carries three payloads and Ocean Colour Monitor (OCM-2), is one of them. OCM-2 is a push broom sensor designed with 3730 active detectors. Along track striping caused by poorly characterized detector to detector calibration results in derived ocean colour products that are less useful. OCM-2 has eight bands, in which first two bands intermittently show vertical striping in the radiometrically corrected data. An algorithm based on sequential scale factor estimation is proposed to remove the vertical striping with minimum change (+/- 2 percent) in radiance. This paper outlines an automatic de-striping algorithm (ADM), discusses the improvement, quantifies the percentage change and describes the limitations of the method.

  18. Genomic analyses and expression evaluation of thaumatin-like gene family in the cacao fungal pathogen Moniliophthora perniciosa.

    Science.gov (United States)

    Franco, Sulamita de Freitas; Baroni, Renata Moro; Carazzolle, Marcelo Falsarella; Teixeira, Paulo José Pereira Lima; Reis, Osvaldo; Pereira, Gonçalo Amarante Guimarães; Mondego, Jorge Maurício Costa

    2015-10-30

    Thaumatin-like proteins (TLPs) are found in diverse eukaryotes. Plant TLPs, known as Pathogenicity Related Protein (PR-5), are considered fungal inhibitors. However, genes encoding TLPs are frequently found in fungal genomes. In this work, we have identified that Moniliophthora perniciosa, a basidiomycete pathogen that causes the Witches' Broom Disease (WBD) of cacao, presents thirteen putative TLPs from which four are expressed during WBD progression. One of them is similar to small TLPs, which are present in phytopathogenic basidiomycete, such as wheat stem rust fungus Puccinia graminis. Fungi genomes annotation and phylogenetic data revealed a larger number of TLPs in basidiomycetes when comparing with ascomycetes, suggesting that these proteins could be involved in specific traits of mushroom-forming species. Based on the present data, we discuss the contribution of TLPs in the combat against fungal competitors and hypothesize a role of these proteins in M. perniciosa pathogenicity.

  19. An improved algorithm for de-striping of ocean colour monitor imageries aided by measured sensor characteristics

    Science.gov (United States)

    Dutt, Ashutosh; Mishra, Ashish; Goswami, D. R.; Kumar, A. S. Kiran

    2016-05-01

    The push-broom sensors in bands meant to study oceans, in general suffer from residual non uniformity even after radiometric correction. The in-orbit data from OCM-2 shows pronounced striping in lower bands. There have been many attempts and different approaches to solve the problem using image data itself. The success or lack of it of each algorithm lies on the quality of the uniform region identified. In this paper, an image based destriping algorithm is presented with constraints being derived from Ground Calibration exercise. The basis of the methodology is determination of pixel to pixel non-uniformity through uniform segments identified and collected from large number of images, covering the dynamic range of the sensor. The results show the effectiveness of the algorithm over different targets. The performance is qualitatively evaluated by visual inspection and quantitatively measured by two parameters.

  20. Investigation and Characterisation of Resizeable Nanopores in an Elastomeric Membrane

    Science.gov (United States)

    Willmott, Geoff

    2008-03-01

    Experimental and theoretical work relating to the development of resizeable synthetic nanopores will be presented. The nanopores, which are roughly conical, are formed by puncturing a relatively thick (˜250 μm) elastomeric membrane with an STM tip. The aperture can be closed and the size can be dynamically controlled by stretching the elastomer [1]. Use of this technology presents a collection of interesting physical problems, covering topics that include the failure and mechanical properties of the elastomer, flow of ionic current through the aperture and particle sensing using the resistive pulse technique. Synthetic nanopores have potential applications in many fields, but especially relating to nanoscale sensing and diagnostic devices, and replication of ion channels in living cells. [1] S. J. Sowerby, M. F. Broom, G. B. Petersen, Dynamically Resizable Nanometre-Scale Apertures for Molecular Sensing, Sensors and Actuators B: Chemical 123 (1), pp. 325-330 (2007)

  1. Reduced memory multi-layer multi-component rate allocation for JPEG2000

    Science.gov (United States)

    Kulkarni, Prajit; Bilgin, Ali; Marcellin, Michael W.; Dagher, Joseph C.; Flohr, Thomas; Rountree, Janet

    2005-03-01

    Remote sensing images are often multispectral in nature and are acquired by on-board sensors in a "push-broom" fashion. These images are compressed and transmitted to ground stations for further analysis. Since they are extremely large, buffering all acquired data before encoding requires huge amounts of memory and introduces latency. Incremental compression schemes work on small chunks of raw data as soon as they are acquired and help reduce buffer memory requirements. However, incremental processing leads to large variations in quality across the reconstructed image. We propose two "leaky bucket" rate control algorithms that can be employed for incrementally compressing hyperspectral images using JPEG2000. Both schemes perform rate control using the fine granularity afforded by JPEG2000. The proposed algorithms have low memory requirements and enable SNR scalability through the use of quality layers. Experiments show that the proposed schemes provide significant reduction in quality variation with no loss in mean overall PSNR performance.

  2. Spectrum reconstruction using relative-deviation-based kernel regression in temporally and spatially modulated Fourier transform imaging spectrometer.

    Science.gov (United States)

    Huang, Fengzhen; Yuan, Yan; Li, Jingzhen; Cao, Jun

    2015-08-01

    During the temporally and spatially modulated Fourier transform imaging spectrometer push-broom scanning process, the motion state of the spectrometer platform can vary. Thus, the target interferogram obtained from the image sequence deviates from the ideal interferogram obtained using high platform stability. The recovered target spectrum will not reflect the true target characteristics. We adopted target tracking to acquire the target position in the image sequence via a proposed kernel regression, with a relative deviation method for determining the target intensities, and the recovery of the spectrogram using the nonuniform fast Fourier transform algorithm. We tested our algorithm on simulated and experimentally obtained aerial images and, from comparison with accurate spectrograms, demonstrate the effectiveness of the proposed method.

  3. Development, Cultural Maintenance and Traditional Owners: The LNG Development Proposal at James Price Point in the Kimberley

    Directory of Open Access Journals (Sweden)

    Deborah Ruiz Wall

    2010-04-01

    Full Text Available Deep divisions have surfaced amongst Indigenous communities over the proposal to establish a Liquefied Natural Gas (LNG precinct in the Kimberley, 60 km north of Broome. While ‘cultural survival’ underpins the argument put forward by both Indigenous supporters and critics of this development proposal, they appear to have opted for very different paths. A key question is: how do Indigenous people negotiate agreements with the State and mining companies in such a way as to maintain their distinct cultural understandings of themselves and accumulate adequate financial resources to safeguard their cultural survival? This paper examines the experiences of more recent native title settlements to see how they relate to the Kimberley LNG development discourses; interprets sample texts in relation to cultural maintenance within the prevailing dominant Western economic social system; and finally, raise questions about the challenges ahead for traditional owners.

  4. Bonneville Power Administration, Lower Columbia Region: Noxious Weed Management, 1996 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    United States. Bonneville Power Administration, Portland, OR; Oregon Department of Agriculture Noxious Weed Control Program

    1996-01-01

    During the 1996 season ODA executed the contract between BPA and ODA. Execution of this contract included the following activities: Survey for target noxious weeds, such as Gorse; collection and redistribution of biological control agents, for example, Apion seed weevils for Scotch broom, bioagents for diffuse and spotted knapweed, Gorse spider mite, and gall fly releases for control of Canada thistle and bull thistle; and control of isolated infestations of Gorse on BPA rights-of-way. Training was provided for line crews at the Chemawa, Alevy and North Bend districts. The purpose of the program is to assist BPA in the integrated prevention and control of noxious weed species on BPA transmission line maintenance right-of-ways.

  5. Microwave Radiometry and Radiometers for Ocean Applications

    DEFF Research Database (Denmark)

    Skou, Niels

    2008-01-01

    The microwave radiometer system measures, within its bandwidth, the naturally emitted radiation – the brightness temperature – of substances within its antenna’s field of view. Thus a radiometer is really a sensitive and calibrated microwave receiver. The radiometer can be a basic total power....../antenna size, and the problem: scanning antenna/space- craft stability. In many cases good compromises have been reached, as evident recalling the many successful missions throughout the recent 30 years. But in some cases the situation calls for special solutions, like the push-broom system or the synthetic...... aperture radiometer technique, both yielding imaging capability without scanning. Typical applications of microwave radiometry concerning oceans are: sea salinity, sea surface temperature, wind speed and direction, sea ice detection and classification. However, in an attempt to measure properties...

  6. New generation VNIR/SWIR/TIR airborne imaging spectrometer

    Science.gov (United States)

    Wang, Yueming; Wei, Liqin; Yuan, Liyin; Li, Chunlai; Lv, Gang; Xie, Feng; Han, Guicheng; Shu, Rong; Wang, Jianyu

    2016-10-01

    Imaging spectrometer plays an important role in the remote sensing application. Imaging spectrometer can collects and provides a unique spectral signature of many materials. The spectral signature may be absorbing, reflecting, and emitting. Generally, optical spectral bands for earth observing consist of VNIR, SWIR, TIR/LWIR. VNIR band imaging spectrometer is well-known in vegetation remote sensing and ocean detection. SWIR band imaging spectrometer is widely applied in mineralogy investigation. For its uniquely capability of spectral radiance measurement, TIR/LWIR imaging spectrometer attracts much attention these years. This paper will present a new generation VNIR/SWIR/TIR imaging spectrometer. The preliminary result of its first flight will also be shared. The spectral sampling intervals of VNIR/SWIR/TIR are 2.4nm/3nm/30nm, respectively. The spatial pixel numbers are 2800/1400/700,respectively. It's a push-broom imaging spectrometer.

  7. Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers.

    Science.gov (United States)

    Sharafi, Ata Allah; Abkenar, Asad Asadi; Sharafi, Ali; Masaeli, Mohammad

    2016-01-01

    Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime "witches' broom disease".

  8. Shrub control by browsing: Targeting adult plants

    Science.gov (United States)

    da Silveira Pontes, Laíse; Magda, Danièle; Gleizes, Benoît; Agreil, Cyril

    2016-01-01

    Reconciling the well known benefits of shrubs for forage with environmental goals, whilst preventing their dominance, is a major challenge in rangeland management. Browsing may be an economical solution for shrubby rangelands as herbivore browsing has been shown to control juvenile shrub growth. Less convincing results have been obtained for adult plants, and long-term experiments are required to investigate the cumulative effects on adult plants. We therefore assessed the impact of different levels of browsing intensity on key demographic parameters for a major dominant shrub species (broom, Cytisus scoparius), focusing on adult plants. We assigned individual broom plants to one of three age classes: 3-5 years (young adults); 5-7 years (adults); and 7-9 years (mature adults). These plants were then left untouched or had 50% or 90% of their total edible stem biomass removed in simulated low-intensity and high-intensity browsing treatments, respectively. Morphological, survival and fecundity data were collected over a period of four years. Browsing affected the morphology of individual plants, promoting changes in subsequent regrowth, and decreasing seed production. The heavily browsed plants were 17% shorter, 32% narrower, and their twigs were 28% shorter. Light browsing seemed to control the growth of young adult plants more effectively than that of older plants. Reproductive output was considerably lower than for control plants after light browsing, and almost 100% lower after heavy browsing. High-intensity browsing had a major effect on survival causing high levels of plant mortality. We conclude that suitable browsing practices could be used to modify adult shrub demography in the management of shrub dominance and forage value.

  9. TcCYPR04, a Cacao Papain-Like Cysteine-Protease Detected in Senescent and Necrotic Tissues Interacts with a Cystatin TcCYS4.

    Science.gov (United States)

    Cardoso, Thyago Hermylly Santana; Freitas, Ana Camila Oliveira; Andrade, Bruno Silva; Sousa, Aurizangela Oliveira de; Santiago, André da Silva; Koop, Daniela Martins; Gramacho, Karina Peres; Alvim, Fátima Cerqueira; Micheli, Fabienne; Pirovani, Carlos Priminho

    2015-01-01

    The interaction amongst papain-like cysteine-proteases (PLCP) and their substrates and inhibitors, such as cystatins, can be perceived as part of the molecular battlefield in plant-pathogen interaction. In cacao, four cystatins were identified and characterized by our group. We identified 448 proteases in cacao genome, whereof 134 were cysteine-proteases. We expressed in Escherichia coli a PLCP from cacao, named TcCYSPR04. Immunoblottings with anti-TcCYSPR04 exhibited protein increases during leaf development. Additional isoforms of TcCYSPR04 appeared in senescent leaves and cacao tissues infected by Moniliophthora perniciosa during the transition from the biotrophic to the saprophytic phase. TcCYSPR04 was induced in the apoplastic fluid of Catongo and TSH1188 cacao genotypes, susceptible and resistant to M. perniciosa, respectively, but greater intensity and additional isoforms were observed in TSH1188. The fungal protein MpNEP induced PLCP isoform expression in tobacco leaves, according to the cross reaction with anti-TcCYSPR04. Several protein isoforms were detected at 72 hours after treatment with MpNEP. We captured an active PLCP from cacao tissues, using a recombinant cacao cystatin immobilized in CNBr-Sepharose. Mass spectrometry showed that this protein corresponds to TcCYSPR04. A homology modeling was obtained for both proteins. In order to become active, TcCYSPR04 needs to lose its inhibitory domain. Molecular docking showed the physical-chemical complementarities of the interaction between the cacao enzyme and its inhibitor. We propose that TcCYSPR04 and its interactions with cacao cystatins are involved in the senescence and necrosis events related to witches' broom symptoms. This molecular interaction may be the target for future interventions to control witches' broom disease.

  10. Research and implementation of simulation for TDICCD remote sensing in vibration of optical axis

    Science.gov (United States)

    Liu, Zhi-hong; Kang, Xiao-jun; Lin, Zhe; Song, Li

    2013-12-01

    During the exposure time, the charge transfer speed in the push-broom direction and the line-by-lines canning speed of the sensor are required to match each other strictly for a space-borne TDICCD push-broom camera. However, as attitude disturbance of satellite and vibration of camera are inevitable, it is impossible to eliminate the speed mismatch, which will make the signal of different targets overlay each other and result in a decline of image resolution. The effects of velocity mismatch will be visually observed and analyzed by simulating the degradation of image quality caused by the vibration of the optical axis, and it is significant for the evaluation of image quality and design of the image restoration algorithm. How to give a model in time domain and space domain during the imaging time is the problem needed to be solved firstly. As vibration information for simulation is usually given by a continuous curve, the pixels of original image matrix and sensor matrix are discrete, as a result, they cannot always match each other well. The effect of simulation will also be influenced by the discrete sampling in integration time. In conclusion, it is quite significant for improving simulation accuracy and efficiency to give an appropriate discrete modeling and simulation method. The paper analyses discretization schemes in time domain and space domain and presents a method to simulate the quality of image of the optical system in the vibration of the line of sight, which is based on the principle of TDICCD sensor. The gray value of pixels in sensor matrix is obtained by a weighted arithmetic, which solves the problem of pixels dismatch. The result which compared with the experiment of hardware test indicate that this simulation system performances well in accuracy and reliability.

  11. In-Depth Transcriptome Sequencing of Mexican Lime Trees Infected with Candidatus Phytoplasma aurantifolia.

    Directory of Open Access Journals (Sweden)

    Mohsen Mardi

    Full Text Available Witches' broom disease of acid lime greatly affects the production of Mexican lime in Iran. It is caused by a phytoplasma (Candidatus Phytoplasma aurantifolia. However, the molecular mechanisms that underlie phytoplasma pathogenicity and the mode of interactions with host plants are largely unknown. Here, high-throughput transcriptome sequencing was conducted to explore gene expression signatures associated with phytoplasma infection in Mexican lime trees. We assembled 78,185 unique transcript sequences (unigenes with an average length of 530 nt. Of these, 41,805 (53.4% were annotated against the NCBI non-redundant (nr protein database using a BLASTx search (e-value ≤ 1e-5. When the abundances of unigenes in healthy and infected plants were compared, 2,805 transcripts showed significant differences (false discovery rate ≤ 0.001 and log2 ratio ≥ 1.5. These differentially expressed genes (DEGs were significantly enriched in 43 KEGG metabolic and regulatory pathways. The up-regulated DEGs were mainly categorized into pathways with possible implication in plant-pathogen interaction, including cell wall biogenesis and degradation, sucrose metabolism, secondary metabolism, hormone biosynthesis and signalling, amino acid and lipid metabolism, while down-regulated DEGs were predominantly enriched in ubiquitin proteolysis and oxidative phosphorylation pathways. Our analysis provides novel insight into the molecular pathways that are deregulated during the host-pathogen interaction in Mexican lime trees infected by phytoplasma. The findings can be valuable for unravelling the molecular mechanisms of plant-phytoplasma interactions and can pave the way for engineering lime trees with resistance to witches' broom disease.

  12. In-Depth Transcriptome Sequencing of Mexican Lime Trees Infected with Candidatus Phytoplasma aurantifolia.

    Science.gov (United States)

    Mardi, Mohsen; Karimi Farsad, Laleh; Gharechahi, Javad; Salekdeh, Ghasem Hosseini

    2015-01-01

    Witches' broom disease of acid lime greatly affects the production of Mexican lime in Iran. It is caused by a phytoplasma (Candidatus Phytoplasma aurantifolia). However, the molecular mechanisms that underlie phytoplasma pathogenicity and the mode of interactions with host plants are largely unknown. Here, high-throughput transcriptome sequencing was conducted to explore gene expression signatures associated with phytoplasma infection in Mexican lime trees. We assembled 78,185 unique transcript sequences (unigenes) with an average length of 530 nt. Of these, 41,805 (53.4%) were annotated against the NCBI non-redundant (nr) protein database using a BLASTx search (e-value ≤ 1e-5). When the abundances of unigenes in healthy and infected plants were compared, 2,805 transcripts showed significant differences (false discovery rate ≤ 0.001 and log2 ratio ≥ 1.5). These differentially expressed genes (DEGs) were significantly enriched in 43 KEGG metabolic and regulatory pathways. The up-regulated DEGs were mainly categorized into pathways with possible implication in plant-pathogen interaction, including cell wall biogenesis and degradation, sucrose metabolism, secondary metabolism, hormone biosynthesis and signalling, amino acid and lipid metabolism, while down-regulated DEGs were predominantly enriched in ubiquitin proteolysis and oxidative phosphorylation pathways. Our analysis provides novel insight into the molecular pathways that are deregulated during the host-pathogen interaction in Mexican lime trees infected by phytoplasma. The findings can be valuable for unravelling the molecular mechanisms of plant-phytoplasma interactions and can pave the way for engineering lime trees with resistance to witches' broom disease.

  13. Nature wonders at arboretum of Petrozavodsk State University

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    Eglacheva Arina

    2016-12-01

    Full Text Available The Arboretum is the oldest collection of the Botanic garden of the Petrozavodsk State University. Over the past 65 years, the trees have grown, and the landscape ideas of its creators have gained forms (Lantratova and other, 2001. Within Karelia, this is considered an exclusive place with a collection of high-steady wood plants and a possibility of their further use in park construction. The natural environment proved to be successful because of its harmonious location and symbiotic ties. It is safe to say that "the park in the woods" and all things related to it are defined by this formulation. Among the natural wonders (Kunststücks in German of the garden are: outgrowths on trunks (warts, knags, witche's brooms, self-grafting, grafting, decay wound, stilt roots and others. Another highlight of the garden is its hospitality towards the fauna: the bears, wasps, ants, squirrels, hares, and other animals live in freedom within its territory. The above emphasizes the integral value of an arboretum within the ecological education and knowledge of the world.it is harmoniously entered and has symbiotic ties. It is possible to tell safely "the park in the wood" about arboretum and a lot of things are defined by this formulation that the person is capable to see here. Material is devoted to nature wonders (kunststück of a Botanic garden: outgrowths on trunks (wart, knag, witch's broom, ablactation, grafting, the decay’s wound, buttresses and another. One more highlight of a garden is his hospitality for fauna. Bears, wasps, ants, squirrels, hares, and other animals freely feel at this territory. All of that emphasizes the integral value of an arboretum in ecological education and knowledge of the world.

  14. A pharmacological and biochemical examination of the geographical variation of Chironex fleckeri venom.

    Science.gov (United States)

    Winter, Kelly L; Isbister, Geoffrey K; McGowan, Sheena; Konstantakopoulos, Nicki; Seymour, Jamie E; Hodgson, Wayne C

    2010-02-15

    Chironex fleckeri (box jellyfish) are found in the northern tropical waters of Australia. Although C. fleckeri have a wide geographical distribution and are able to swim large distances, adults tend to stay in small restricted areas. Clinical data shows that deaths from envenoming have not been recorded in Western Australia, yet numerous fatalities have occurred in Northern Territory and Queensland waters. One explanation for this discrepancy is a geographical variation in venom composition. This study examined the pharmacological and biochemical profiles of C. fleckeri venom from different geographical locations and seasons. Venoms were screened for cytotoxicity using a rat aortic smooth muscle cell line (A7r5). While all venoms caused concentration-dependent cytotoxicity, differences were seen in the potency of venoms from Mission Beach and Weipa, when collected in different seasons, as indicated by IC(50) values. Similarly venoms collected within the same season, from different locations around Australia, displayed marked differences in venom composition as shown by size exclusion HPLC and SDS-PAGE profiles which indicated the absence or reduced quantity of 'peaks' in some venoms. Based on IC(50) data obtained from the cell assay, the effects of the most potent (i.e. from Weipa in 2006) and the least potent (i.e. from Broome in 2007) venoms were examined in anesthetised rats. Both venoms at 10 microg/kg (i.v.) caused a transient hypertensive phase followed by cardiovascular collapse. However, at 4 microg/kg (i.v.) venom from Weipa 2006 caused a transient hypertensive phase followed by a transient decrease in MAP while venom from Broome 2007 only caused a small transient increase in MAP. This study demonstrates that there is considerable geographical variation in the composition of C. fleckeri venoms which is most distinct between specimens from western and eastern Australia and may explain the geographical variation in reported deaths.

  15. Herbivores influence the growth, reproduction, and morphology of a widespread Arctic willow.

    Science.gov (United States)

    Christie, Katie S; Ruess, Roger W; Lindberg, Mark S; Mulder, Christa P

    2014-01-01

    Shrubs have expanded in Arctic ecosystems over the past century, resulting in significant changes to albedo, ecosystem function, and plant community composition. Willow and rock ptarmigan (Lagopus lagopus, L. muta) and moose (Alces alces) extensively browse Arctic shrubs, and may influence their architecture, growth, and reproduction. Furthermore, these herbivores may alter forage plants in such a way as to increase the quantity and accessibility of their own food source. We estimated the effect of winter browsing by ptarmigan and moose on an abundant, early-successional willow (Salix alaxensis) in northern Alaska by comparing browsed to unbrowsed branches. Ptarmigan browsed 82-89% of willows and removed 30-39% of buds, depending on study area and year. Moose browsed 17-44% of willows and browsed 39-55% of shoots. Browsing inhibited apical dominance and activated axillary and adventitious buds to produce new vegetative shoots. Ptarmigan- and moose-browsed willow branches produced twice the volume of shoot growth but significantly fewer catkins the following summer compared with unbrowsed willow branches. Shoots on browsed willows were larger and produced 40-60% more buds compared to unbrowsed shoots. This process of shoot production at basal parts of the branch is the mechanism by which willows develop a highly complex "broomed" architecture after several years of browsing. Broomed willows were shorter and more likely to be re-browsed by ptarmigan, but not moose. Ptarmigan likely benefit from the greater quantity and accessibility of buds on previously browsed willows and may increase the carrying capacity of their own habitat. Despite the observed tolerance of willows to browsing, their vertical growth and reproduction were strongly inhibited by moose and ptarmigan. Browsing by these herbivores therefore needs to be considered in future models of shrub expansion in the Arctic.

  16. An echelle diffraction grating for imaging spectrometer

    Science.gov (United States)

    Yang, Minyue; Wang, Han; Li, Mingyu; He, Jian-Jun

    2016-09-01

    We demonstrate an echelle diffraction grating (EDG) of 17 input waveguides and 33 output waveguides. For each input waveguide, only 17 of 33 output waveguides are used, receiving light ranging from 1520 nm to 1600 nm wavelength. The channel spacing of the EDG is 5 nm, with loss of -6dB and crosstalk of -17dB for center input waveguide and -15dB for edge input waveguides. Based on the 3 μm SOI platform the device is polarization insensitive. As a simple version of EDG spectrometer it is designed to be a part of the on-chip spectroscopic system of the push-broom scanning imaging spectrometer. The whole on-chip spectrometer consists of an optical on-off switch array, a multi-input EDG and detector array. With the help of on-off switch array the multiple input waveguides of the EDG spectrometer could work in a time division multiplexed fashion. Since the switch can scan very fast (less than 10 microseconds), the imaging spectrometer can be operated in push-broom mode. Due to the CMOS compatibility, the 17_channel EDG scales 2.5×3 mm2. The full version of EDG spectrometer is designed to have 129 input waveguides and 257 output waveguides (129 output channel for each input waveguide), working in wavelength ranging from 1250 nm to 1750 nm, and had similar blazed facet size with the 17_channel one, which means similar fabrication tolerance in grating facets. The waveguide EDG based imaging spectrometer can provide a low-cost solution for remote sensing on unmanned aerial vehicles, with advantages of small size, light weight, vibration-proof, and high scalability.

  17. Cross-Comparison of Vegetation Indices Derived from Landsat-7 Enhanced Thematic Mapper Plus (ETM+ and Landsat-8 Operational Land Imager (OLI Sensors

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    Peng Li

    2013-12-01

    Full Text Available Landsat-7 Enhanced Thematic Mapper Plus (ETM+ and Landsat-8 Operational Land Imager (OLI and Thermal Infrared Sensor (TIRS are currently operational for routine Earth observation. There are substantial differences between instruments onboard both satellites. The enhancements achieved with Landsat-8 refer to the scanning technology (replacing of whisk-broom scanners with two separate push-broom OLI and TIRS scanners, an extended number of spectral bands (two additional bands provided and narrower bandwidths. Therefore, cross-comparative analysis is very necessary for the combined use of multi-decadal Landsat imagery. In this study, 3,311 independent sample points of four major land cover types (primary forest, unplanted cropland, swidden cultivation and water body were used to compare the spectral bands of ETM+ and OLI. Eight sample plots with different land cover types were manually selected for comparison with the Normalized Difference Vegetation Index (NDVI, the Modified Normalized Difference Water Index (MNDWI, the Land Surface Water Index (LSWI and the Normalized Burn Ratio (NBR. These indices were calculated with six pairs of ETM+ and OLI cloud-free images, which were acquired over the border area of Myanmar, Laos and Thailand just two days apart, when Landsat-8 achieved operational obit. Comparative results showed that: (1 the average surface reflectance of each band differed slightly, but with a high degree of similarities between both sensors. In comparison with ETM+, the OLI had higher values for the near-infrared band for vegetative land cover types, but lower values for non-vegetative types. The new sensor had lower values for the shortwave infrared (2.11–2.29 µm band for all land cover types. In addition, it also basically had higher values for the shortwave infrared (1.57–1.65 µm band for non-water land cover types. (2 The subtle differences of vegetation indices derived from both sensors and their high linear correlation

  18. 360°直肠腔内超声检查在肛瘘的应用研究——直肠肛管横断面解剖对肛瘘诊疗的指导%360° rectalendosonography in anal fistula applied research-Rectum and anal canal cross-sectional anatomy of the guidance on diagnosis and treatment of anal fistula

    Institute of Scientific and Technical Information of China (English)

    张秀; 秦澎湃; 熊芳; 黄斌; 李志祥

    2012-01-01

    目的 探讨360°直肠腔内超声检查在肛瘘诊疗中的应用价值.方法 200例拟手术治疗的肛瘘患者随机进行两种直肠腔内超声检查,其中103例使用360°旋转探头(旋转组),97例应用端扫式凸阵探头(端扫组),临床医师参考超声报告设计手术方案进行手术,分析两种检查方法的结果.结果 两组在诊断肛瘘的灵敏度上差异无统计学意义,对指导临床医师诊断肛瘘及手术的帮助作用存在差异,有统计学意义.结论 360°旋转探头与端扫式腔内探头在肛瘘的诊断灵敏度均较高,但是360°旋转探头超声能更准确的指导临床医师制定手术方案,提高手术成功率.%Objective For patients with anal fistula rectal cavity of 360?rotating ultrasonic probe examination, anorectal and get continuous cross-sectional ultrasound images of the surrounding tissue, cross sectional shape changes hy clinicians to determine the location of the Traveling anal fistula, guided surgery. Methods 200 cases of anal fistula surgery patients to he randomized to two kinds of transrectal ultrasound, in which 103 cases with 360° rotating probe, 97 patients were treated side-hroom convex array probe, clinicians report reference ultrasound surgery surgery program design, analysis, two types of inspection Methods results. Results 360° rotating cavity with side broom cavity probe sensor sensitivity in the diagnosis of anal fistula were no statistically significant difference, to guide clinicians and diagnosis of anal fistula surgery with the help of role differences, there are statistically significant. Conclusion 360° rotating probe with side broom cavity probe sensitivity in the diagnosis of anal fistula were higher, but the 360° rotating ultrasound probe to guide clinicians to more accurate surgical planning, surgical guidance.

  19. Common handling procedures conducted in preclinical safety studies result in minimal hepatic gene expression changes in Sprague-Dawley rats.

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    Yudong D He

    Full Text Available Gene expression profiling is a tool to gain mechanistic understanding of adverse effects in response to compound exposure. However, little is known about how the common handling procedures of experimental animals during a preclinical study alter baseline gene expression. We report gene expression changes in the livers of female Sprague-Dawley rats following common handling procedures. Baseline gene expression changes identified in this study provide insight on how these changes may affect interpretation of gene expression profiles following compound exposure. Rats were divided into three groups. One group was not subjected to handling procedures and served as controls for both handled groups. Animals in the other two groups were weighed, subjected to restraint in Broome restrainers, and administered water via oral gavage daily for 1 or 4 days with tail vein blood collections at 1, 2, 4, and 8 hours postdose on days 1 and 4. Significantly altered genes were identified in livers of animals following 1 or 4 days of handling when compared to the unhandled animals. Gene changes in animals handled for 4 days were similar to those handled for 1 day, suggesting a lack of habituation. The altered genes were primarily immune function related genes. These findings, along with a correlating increase in corticosterone levels suggest that common handling procedures may cause a minor immune system perturbance.

  20. STRIPING NOISE REMOVAL OF IMAGES ACQUIRED BY CBERS 2 CCD CAMERA SENSOR

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    E. Amraei

    2014-10-01

    Full Text Available CCD Camera is a multi-spectral sensor that is carried by CBERS 2 satellite. Imaging technique in this sensor is push broom. In images acquired by the CCD Camera, some vertical striping noise can be seen. This is due to the detectors mismatch, inter detector variability, improper calibration of detectors and low signal-to-noise ratio. These noises are more profound in images acquired from the homogeneous surfaces, which are processed at level 2. However, the existence of these noises render the interpretation of the data and extracting information from these images difficult. In this work, spatial moment matching method is proposed to modify these images. In this method, the statistical moments such as mean and standard deviation of columns in each band are used to balance the statistical specifications of the detector array to those of reference values. After the removal of the noise, some periodic diagonal stripes remain in the image where their removal by using the aforementioned method seems impossible. Therefore, to omit them, frequency domain Butterworth notch filter was applied. Finally to evaluate the results, the image statistical moments such as the mean and standard deviation were deployed. The study proves the effectiveness of the method in noise removal.

  1. Diversity of macrofungal genus Russula and Amanita in Hirpora Wildlife Sanctuary, Southern Kashmir Himalayas

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    SHAUKET AHMED PALA

    2012-04-01

    Full Text Available Pala SA, Wani AH, Mir RA. 2012. Diversity of macrofungal genus Russula and Amanita in Hirpora Wild Life Sancturary. Biodiversitas 13: 65-71. The Hirpora Wildlife Sanctuary that extends over an area of 114 km2 lies in the Pir Panjal range at a distance of 70 km in south-west of summer capital Srinagar. It is rich in biodiversity including macrofungal diversity. The Sanctuary has been subjected to high ecological and anthropogenic disturbance due to the construction of Mughal road which is major threat for its biodiversity. Since there is hardly any report of documentation of macrofungi from this sanctuary. In this back drop a survey was carried out during the year 2010 and 2011 to explore and invetorise macrofungal diversity of the sanctuary. During the survey a no of macrofungi were documented, among which Amanita and Russula were dominant genus represented by 7 species each. All the 14 species viz. Amanita ceciliae (Berk. & Broome Bas. Amanita flavoconia G.F. Atk., Amanita muscaria var. formosa Pers., Amanita pantherina (Fr. Krombh., Amanita phalloides (Fr. Link., Amanita vaginata (Bull. ex Fr. Vitt., Amanita virosa (Fr. Bertillon, Russula aeruginea Fr., Russula atropurpurea (Krombh. Britz., Russula aurea Pers., Russula cyanoxantha (Schaeff. Fr., Russula delica Fr. Russula emetica (Schaeff. ex Fr. Gray. and Russula nobilis Velen. are ectomycorrhizal in nature and among them Russula aeruginea Fr. is reported first time from the Kashmir.

  2. The Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS) on the Landsat Data Continuity Mission (LDCM)

    Science.gov (United States)

    Reuter, Dennis; Irons, James; Lunsford, Allen; Montanero, Matthew; Pellerano, Fernando; Richardson, Cathleen; Smith, Ramsey; Tesfaye, Zelalem; Thome, Kurtis

    2011-01-01

    The Landsat Data Continuity Mission (LDCM), a joint NASA and United States Geological Survey (USGS) mission, is scheduled for launch in December, 2012. The LDCM instrument payload will consist of the Operational Land Imager (OLI), provided by Ball Aerospace and Technology Corporation (BATC) under contract to NASA and the Thermal Infrared Sensor (TIRS), provided by NASA's Goddard Space Flight Center (GSFC). This paper will describe the design, capabilities and status of the OLI and TIRS instruments. The OLI will provide 8 channel multispectral images at a spatial resolution of 30 meters and panchromatic images at 15 meter spatial resolution. The TIRS is a 100 meter spatial resolution push-broom imager whose two spectral channels, centered at 10.8 and 12 microns, split the ETM+ thermal bands. The two channels allow the use of the "split-window" technique to aid in atmospheric correction. The TIRS focal plane consists of three Quantum Well Infrared Photodetector (QWIP) arrays to span the 185 km swath width. The OLI and TIRS instruments will be operated independently but in concert with each other. Data from both instruments will be merged into a single data stream at the (USGS)/Earth Resources Observation and Science (EROS) facility. The ground system, being developed by USGS, includes an Image Assessment System (lAS), similar to Landsat-7's, to operationally monitor, characterize and update the calibrations of the two sensors.

  3. The use of piassava fibers (Attalea funifera) in the preparation of activated carbon.

    Science.gov (United States)

    Avelar, Fabiana Ferreira; Bianchi, Maria Lúcia; Gonçalves, Maraisa; da Mota, Estella Gaspar

    2010-06-01

    The piassava fiber, residue of the broom industry, was used as precursor for the preparation of activated carbons (AC). AC were prepared by chemical activation with zinc chloride (AC ZnCl(2)) or phosphoric acid (AC H(3)PO(4)) and by physical activation with carbon dioxide (AC CO(2)) or water vapor (AC H(2)O). These materials were characterized by adsorption/desorption of N(2) to determine the BET areas, elemental analysis (CHN), thermogravimetric analysis (TG, DTA) and scanning electron microscopy (SEM). The carbons were tested with respect to their adsorption capacity of methylene blue, reactive red, phenol and metallic ions (Cr(+6), Cu(+2) and Zn(+2)). AC ZnCl(2) presented the highest surface area (1190 m(2)g(-1)) and AC H(3)PO(4), the largest pore volume (0.543 cm(3)g(-1)). AC ZnCl(2) was more efficient in the adsorption of methylene blue, Cr(+6) and Cu(+2) ions. AC H(2)O was the better adsorbent for phenol, while AC CO(2) was better for Zn(+2) ions.

  4. High Resolution Airborne Laser Scanning and Hyperspectral Imaging with a Small Uav Platform

    Science.gov (United States)

    Gallay, Michal; Eck, Christoph; Zgraggen, Carlo; Kaňuk, Ján; Dvorný, Eduard

    2016-06-01

    The capabilities of unmanned airborne systems (UAS) have become diverse with the recent development of lightweight remote sensing instruments. In this paper, we demonstrate our custom integration of the state-of-the-art technologies within an unmanned aerial platform capable of high-resolution and high-accuracy laser scanning, hyperspectral imaging, and photographic imaging. The technological solution comprises the latest development of a completely autonomous, unmanned helicopter by Aeroscout, the Scout B1-100 UAV helicopter. The helicopter is powered by a gasoline two-stroke engine and it allows for integrating 18 kg of a customized payload unit. The whole system is modular providing flexibility of payload options, which comprises the main advantage of the UAS. The UAS integrates two kinds of payloads which can be altered. Both payloads integrate a GPS/IMU with a dual GPS antenna configuration provided by OXTS for accurate navigation and position measurements during the data acquisition. The first payload comprises a VUX-1 laser scanner by RIEGL and a Sony A6000 E-Mount photo camera. The second payload for hyperspectral scanning integrates a push-broom imager AISA KESTREL 10 by SPECIM. The UAS was designed for research of various aspects of landscape dynamics (landslides, erosion, flooding, or phenology) in high spectral and spatial resolution.

  5. 基于CIPS的DEM匹配的实验研究%The Experimental Study of DEM Matching Based on the CIPS

    Institute of Scientific and Technical Information of China (English)

    陈凤

    2012-01-01

    With the rapid development of city construction and the more and more pressing need of basic geographic information caused by urbanization, Digital Elevation Model is becoming one of the indispensable kinds of basic geographic information, drawing attention to itself in the field of photogrammetric survey with the introduction of ADS 40 push - broom digital sensor. This paper carries out experimental research and precision analysis on DEM matching based on CIPS taking ADS 40 image data of a certain area as a case study.%随着城市建设的快速发展,城市化对基础地理信息的需要日趋迫切,数字高程模型(Digital Elevation Model,DEM)成为不可缺少的基础地理信息之一,并随着ADS40推扫式数字传感器的推广在摄影测量界备受关注。文章以某地区的ADS40影像数据为例,基于CIPS进行了DEM匹配的实验研究与精度分析。

  6. Pathogenic variability of Moniliophthora perniciosa in three agroecological zones of the cacao region of Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Karina Peres Gramacho

    2016-03-01

    Full Text Available Resistant cacao genotypes are the best measure to control the Witches’ broom disease of cacao. To ensure efficiency, the pathogen variability in the cacao region of Bahia must be investigated. The pathogenic variability of Moniliophthora perniciosa inocula from nine municipalities was analyzed: Ilhéus, Floresta Azul and Jussari (agroecological zone Almada; Ipiaú, Itagibá and Jitaúna (agroecological zone Ipiaú, Camacan, Santa Luzia and Pau Brasil (agroecological zone Camacan, by inoculating progenies of Sca 6, ICS 1 and SIC 23 seedlings with 2 x 105 basidiospore.mL-1 of inocula and inoculum mixtures per agroecological zone, in a factorial 3 x 13 design, with 4 replications of 28 plants, in two seasons. Symptoms were assessed 60 days after inoculation. Pathogenic variability of inoculum in relation to the progeny performance was found; Ipiaú was the most aggressive and progeny ICS 1 the most susceptible. The mixtures of the agroecological zones Almada and Ipiaú were contrasting.

  7. Zinc silicates with tunable morphology by surfactant assisted sonochemical route suitable for NUV excitable white light emitting diodes.

    Science.gov (United States)

    Basavaraj, R B; Nagabhushana, H; Daruka Prasad, B; Vijayakumar, G R

    2017-01-01

    The cationic surfactants assisted ultrasound route was used to prepare Dy(3+) doped Zn2SiO4 nanophosphors. The final products were characterized by powder X-ray diffraction (PXRD), ultraviolet visible spectroscopy, scanning electron microscopy, transmission electron microscopy and photoluminescence. Orthorhombic phase of Zn2SiO4:Dy(3+) (JCPDS card No. 35-1485) was confirmed from PXRD. It was evident that the morphology of spherical and broom like structures were obtained with epigallocatechin gallate (EGCG) and cetyltrimethylammonium bromide (CTAB) surfactants respectively. Further the size and agglomeration of the products were varied with surfactants concentration, sonication time, pH and sonication power. The probable formation mechanisms to obtain various micro/nano superstructures were discussed. The characteristic PL peaks were observed at 484, 574 and 666nm due to the electronic transitions (4)F9/2→(6)Hj (j=15/2, 13/2, 11/2) of Dy(3+) ions upon excited at NUV pumping wavelength of 350nm [(6)H15/2→(6)P7/2 ((4)M15/2)]. The Judd-Ofelt intensity parameters and radiative properties were estimated by using PL emission data. The photometric studies indicated that the obtained phosphors could be promising materials in white light emitting diodes (wLED's). The present synthesis route was rapid, environmentally benign, cost-effective and useful for industrial applications such as solid state lighting and display devices.

  8. The control of syndepositional faulting on the Eogene sedimentary basin fills of the Dongying and Zhanhua sags,Bohai Bay Basin

    Institute of Scientific and Technical Information of China (English)

    LIN Changsong; ZHENG Herong; REN Jianye; LIU Jingyan; Qiu Yigang

    2004-01-01

    The Dongying and Zhanhua sags are the major hydrocarbon exploration and production subbasins in the Bohai Bay Basin. Integrated analysis of the sedimentary basin fills has shown that the syndepositional faults and their arrangement styles exerted an important influence on the development and distribution of the Eogene depositional systems. The sedimentary filling evolution of the subbasins reflects the general control of the episodic rifting process. The major long-term active normal faults formed a series of paleogeomorphic accident or slopebreak zones that commonly delineated the subsidiary palaeostructural units and the depositional facies tracts and constrained the general distribution of sedimentary facies zones. The central sag boundary fault slopebreak zones usually determined the distribution of the depocenters of terrestrial clastic depositional systems, particularly the Iowstand fans or deltaic depositional systems, and have proven to be the economically important targets for the exploration of subtle sandstone reservoirs. A variety of syndepositional fault arrangement patterns, including the parallel, en echelon, combo-like, broom-like fault systems and the fault overlap or transfer zones,have been recognized in the subbasins. They generated distinctive geomorphic features and exerted a direct influence on sediment dispersal and sandbody distribution during the Eogene synrift stage. It is the key for the prediction of depositional systems tracts and reservoir sandstones to investigate the activity and distribution of the fault slopebreak zones and reconstruct the structural paleogeomorphy in different basin filling stages of the basin evolution.

  9. Lipid class and fatty acid content of the Leptocephalus larva of tropical eels

    DEFF Research Database (Denmark)

    Deibel, D.; Parrish, C.C.; Grønkjær, P.;

    2012-01-01

    The leptocephalus larva of eels distinguishes the elopomorph fishes from all other bony fishes. The leptocephalus is long lived and increases in size primarily through the synthesis and deposition of glycosaminoglycans. Energy stored during the larval stage, in the form of glycosaminoglycan...... storage and condition of leptocephali, we determined the lipid class and fatty acid concentration of larvae collected on a cross-shelf transect off Broome, northwestern Australia. The total lipid concentration of two families and four sub-families of leptocephali ranged from 2.7 to 7.0 mg g wet weight-1......:0 (23 mol%), 22:6n-3 (docosahexaenoic acid, DHA, 16 mol%), 18:0 (8.2 mol%), 20:5n-3 (eicosapentaenoic acid, EPA, 6.7 mol%), 18:1n-9 (6.4 mol%) and 16:1n-7 (6.3 mol%). The DHA:EPA ratio ranged from 2.4 to 2.9, sufficient for normal growth and development of fish larvae generally. The leptocephali had...

  10. Ten Species of Vam Fungi in Five Ginseng Fields of Jilin Province%吉林省参地中的10种VA菌根真菌

    Institute of Scientific and Technical Information of China (English)

    邢晓科; Dal.,Y

    2000-01-01

    从吉林省主要人参产区的5个参场人参根际土壤中分离得到10种VA菌根真菌: 1.穴状无梗囊霉Acaulospora cavernata Blaszkowski; 2.刺状无梗孢囊霉Acaulospora spinosa Walker & Trapple; 3.束球囊霉Glomus fasciculatum (Thaxter) Gerd.& Trappe; 4.地球囊霉Glomus geosporum (Nicol.& Gerd) Walker; 5.大果球囊霉Glomus macrocarpum Tulasne & Tulasne; 6.微丛球囊霉Glomus microaggregatum Koske; 7.摩西球囊霉Glomus mosseae (Nicolson & Gerdemann) Gerdemann & Trappe;8.帚状硬囊霉Sclerocystis coremioides Berk.& Broome; 9.棒孢硬囊霉Sclerocystis clavispora Trappe; 10.丽孢盾巨孢囊霉Scutellospora calospora (Nicol.& Gerd.) Walker & Sanders.其中穴状无梗囊霉(Acaulospora cavernata)为国内新记录种.文中描述了10种VA菌根真菌形态学特征,并对其频度、优势种类及孢子数量的季节变化等进行了研究.

  11. GOSAT Air Pollution Watch - Rapid Response System for Local Air Pollution

    Science.gov (United States)

    Matsunaga, T.; Sawada, Y.; Kamei, A.; Uchiyama, A.

    2015-12-01

    GOSAT (Greenhouse Gases Observing Satellite) launched in 2009 and its successor, GOSAT-2, to be launched in FY 2017, have push-broom imaging systems with more than one UV band with higher spatial resolution than OMI, MODIS, and VIIRS. Such imaging systems are useful for mapping the spatial extent of the optically thick air mass with particulate matters. GOSAT Air Pollution Watch, a rapid response system mainly using GOSAT CAI (Cloud and Aerosol Imager) data for local air pollution issues is being developed in NIES (National Institute for Environmental Studies) GOSAT-2 Project. The current design of GOSAT Air Pollution Watch has three data processing steps as follows: Step 1) Making a cloud mask Step 2) Estimating AOT (Aerosol Optical Thickness) in the UV region (380 nm for CAI) Step 3) Converting AOT to atmospheric pollution parameters such as PM2.5 concentration Data processing algorithms in GOSAT Air Pollution Watch are based on GOSAT/GOSAT-2 algorithms for aerosol product generation with some modification for faster and timely data processing. Data from GOSAT Air Pollution Watch will be used to inform the general public the current distribution of the polluted air. In addition, they will contribute to short term prediction of the spatial extent of the polluted air using atmospheric transport models. In this presentation, the background, the current status, and the future prospect of GOSAT Air Pollution Watch will be reported together with the development status of GOSAT-2.

  12. Broadening the application of evolutionarily based genetic pest management.

    Science.gov (United States)

    Gould, Fred

    2008-02-01

    Insect- and tick-vectored diseases such as malaria, dengue fever, and Lyme disease cause human suffering, and current approaches for prevention are not adequate. Invasive plants and animals such as Scotch broom, zebra mussels, and gypsy moths continue to cause environmental damage and economic losses in agriculture and forestry. Rodents transmit diseases and cause major pre- and postharvest losses, especially in less affluent countries. Each of these problems might benefit from the developing field of Genetic Pest Management that is conceptually based on principles of evolutionary biology. This article briefly describes the history of this field, new molecular tools in this field, and potential applications of those tools. There will be a need for evolutionary biologists to interact with researchers and practitioners in a variety of other fields to determine the most appropriate targets for genetic pest management, the most appropriate methods for specific targets, and the potential of natural selection to diminish the effectiveness of genetic pest management. In addition to producing environmentally sustainable pest management solutions, research efforts in this area could lead to new insights about the evolution of selfish genetic elements in natural systems and will provide students with the opportunity to develop a more sophisticated understanding of the role of evolutionary biology in solving societal problems.

  13. Effectiveness of the multidimensional ergonomic intervention model to reduce musculoskeletal discomfort among street sweepers in Chiang Rai Province, Thailand

    Directory of Open Access Journals (Sweden)

    Pintakham K

    2016-12-01

    Full Text Available Kanjanar Pintakham,1,2 Wattasit Siriwong1 1College of Public Health Sciences, Chulalongkorn University, Bangkok, 2School of Health Science, Chiang Rai Rajabhat University, Chiang Rai, Thailand Abstract: The aim of this study was to evaluate the effectiveness of multidimensional ergonomic intervention (MEI model to reduce musculoskeletal discomfort (MSD among street sweepers. A quasi-experimental study was conducted in Chiang Rai Province. The MEI model was designed on the basis of four core components: cognitive behavior therapy, ergonomic education training, stretching exercise, and the foam sleeve broom handle grip. Seventy-five street sweepers volunteered for the screening process on MSD of having level score ≥4 by physiotherapist. Face to face interviews were used mainly in order to diagnose MSD. Physical examination was performed by physiotherapist and physical performance by sports scientist. The findings showed that the MEI model among the intervention group significantly reduced MSD compared with that among control group at exit model and follow-up (P<0.01. This research suggests that the MEI model was appropriate to reduce MSD associated with repetitive movement and awkward postures on task. Keywords: repetitive movement, cognitive behavior therapy, stretching exercise, street sweepers

  14. Changes in Al and Fe associated with amorphous soil minerals over one year after a wildfire at Pico Cho Marcial (Tenerife, Canary Islands, Spain

    Directory of Open Access Journals (Sweden)

    J. Notario

    2013-05-01

    Full Text Available Amorphous-linked Al, Fe and Si were determined both in burned and unburnt soil samples after a wildfire close to Pico Cho Marcial (Tenerife, Canary Islands, Spain that affected 7.1 ha of high mountain Teide broom scrub along four successive samplings held in September 2003 (three months after the wildfire, February 2004, June 2004 and October 2004. Soils in the area are Lithic Xerorthents, with a scarcely developed AC-type profile. The chemical elements under study were extracted using selective dissolutions (0.2M acid ammonium oxalate and 0.1N sodium pyrophosphate, and determined by Atomic Absorption Spectrophotometry. The average contents of total amorphous Al (oxalate-extractable and organo-metallic complexes-bound Al (pyrophosphate extractable were significantly higher in burned samples along the study. Also, the oxalate-extractable Al varied significantly along the different samplings, and so consequently did the Alp:Alox ratio. A progressive decrease in the Al:Si ratio in allophanes was also found throughout the study period. No differences were found for oxalate-extractable Fe, either between sample groups or samplings, which was also applicable to the (Alox+0.5Feox percentage.

  15. TIRCIS: thermal infrared compact imaging spectrometer for small satellite applications

    Science.gov (United States)

    Wright, Robert; Lucey, Paul; Crites, Sarah; Garbeil, Harold; Wood, Mark; Pilger, Eric; Gabrieli, Andrea; Honniball, Casey

    2016-10-01

    Measurements of reflectance or emittance in tens of narrow, contiguous wavebands, allow for the derivation of laboratory quality spectra remotely, from which the chemical composition and physical properties of targets can be determined. Although spaceborne (e.g. EO-1 Hyperion) hyperspectral data in the 0.4-2.5 micron (VSWIR) region are available, the provision of equivalent data in the log-wave infrared has lagged behind, there being no currently operational high spatial resolution LWIR imaging spectrometer on orbit. TIRCIS (Thermal Infra-Red Compact Imaging Spectrometer), uses a Fabry-Perot interferometer, an uncooled microbolometer array, and push-broom scanning to acquire hyperspectral image data. Radiometric calibration is provided by blackbody targets while spectral calibration is achieved using monochromatic light sources. The instrument has a mass of <15 kg and dimensions of 53 cm × 25 cm ♢ 22 cm, and has been designed to be compatible with integration into a micro-satellite platform. (A precursor to this instrument was launched onboard a 55 kg microsatellite in October 2015). The optical design yields a 120 m ground sample size given an orbit of 500 km. Over the wavelength interval of 7.5 to 14 microns up to 50 spectral samples are possible. Measured signal-to-noise ratios range from peak values of 500:1 to 1500:1, for source temperature of 10 to 100°C.

  16. Analysis of LAPAN-IPB image lossless compression using differential pulse code modulation and huffman coding

    Science.gov (United States)

    Hakim, P. R.; Permala, R.

    2017-01-01

    LAPAN-A3/IPB satellite is the latest Indonesian experimental microsatellite with remote sensing and earth surveillance missions. The satellite has three optical payloads, which are multispectral push-broom imager, digital matrix camera and video camera. To increase data transmission efficiency, the multispectral imager data can be compressed using either lossy or lossless compression method. This paper aims to analyze Differential Pulse Code Modulation (DPCM) method and Huffman coding that are used in LAPAN-IPB satellite image lossless compression. Based on several simulation and analysis that have been done, current LAPAN-IPB lossless compression algorithm has moderate performance. There are several aspects that can be improved from current configuration, which are the type of DPCM code used, the type of Huffman entropy-coding scheme, and the use of sub-image compression method. The key result of this research shows that at least two neighboring pixels should be used for DPCM calculation to increase compression performance. Meanwhile, varying Huffman tables with sub-image approach could also increase the performance if on-board computer can support for more complicated algorithm. These results can be used as references in designing Payload Data Handling System (PDHS) for an upcoming LAPAN-A4 satellite.

  17. Vegetative and reproductive phenology of Butia purpurascens Glassman (Arecaceae under the effects of leaf harvesting

    Directory of Open Access Journals (Sweden)

    FAG Guilherme

    Full Text Available Butia purpurascens is an endemic and threatened palm tree species that occurs in open areas of the Brazilian Cerrado, predominantly in southwestern Goiás. The leaves of this palm tree are harvested by local people to fabricate brooms. This study evaluated changes in vegetative and reproductive phenology in two different natural populations of this palm tree: one population with leaf harvesting and another non-harvested population. Twenty plants were monitored in each area for 23 months. The phenophases were related to the temperature and precipitation averages for a 30-year period. Leaf sprouting occurred throughout the year, with a slight reduction in periods of low temperatures and low rainfall. The first spathes emerged in March and flowering began during the dry season (June, continuing until January of the following year, concurrent with the period of most intense fruiting. Flowering and fruiting appear to be triggered by periods of drought, which are commonly observed in the Cerrado. The harvested sites produced significantly fewer leaves, spathes, inflorescences and infructescences than the non-harvested sites. Thus, the supply of resources to the local fauna is possibly reduced in sites under leaf exploitation, which in the long term can represent damage to the palm tree population’s structure and dynamics. Other socioeconomic and ecological studies about the effects of leaf harvesting in B. purpurascens are necessary to enable strategies for sustainable use, devise management alternatives and conserve this threatened palm species.

  18. Vegetative and reproductive phenology of Butia purpurascens Glassman (Arecaceae) under the effects of leaf harvesting.

    Science.gov (United States)

    Guilherme, F A G; Vasconcelos, E I; Coelho, C P; Ressel, K; Batista, N T F; Souza, L F

    2015-01-01

    Butia purpurascens is an endemic and threatened palm tree species that occurs in open areas of the Brazilian Cerrado, predominantly in southwestern Goiás. The leaves of this palm tree are harvested by local people to fabricate brooms. This study evaluated changes in vegetative and reproductive phenology in two different natural populations of this palm tree: one population with leaf harvesting and another non-harvested population. Twenty plants were monitored in each area for 23 months. The phenophases were related to the temperature and precipitation averages for a 30-year period. Leaf sprouting occurred throughout the year, with a slight reduction in periods of low temperatures and low rainfall. The first spathes emerged in March and flowering began during the dry season (June), continuing until January of the following year, concurrent with the period of most intense fruiting. Flowering and fruiting appear to be triggered by periods of drought, which are commonly observed in the Cerrado. The harvested sites produced significantly fewer leaves, spathes, inflorescences and infructescences than the non-harvested sites. Thus, the supply of resources to the local fauna is possibly reduced in sites under leaf exploitation, which in the long term can represent damage to the palm tree population's structure and dynamics. Other socioeconomic and ecological studies about the effects of leaf harvesting in B. purpurascens are necessary to enable strategies for sustainable use, devise management alternatives and conserve this threatened palm species.

  19. Spatio-temporal evolution of the optical field on a hohlraum wall at the rising edge of a flat-topped pulse

    Institute of Scientific and Technical Information of China (English)

    Zhaoyang; Jiao; Yanli; Zhang; Junyong; Zhang; Jianqiang; Zhu

    2013-01-01

    Considering the time delay in different hohlraum wall positions caused by oblique incidence,the spatio-temporal optical field distribution characteristics of a hohlraum wall,especially during the rising edge of a flat-topped pulse,is simulated by a fast Fourier transform method together with chromatography.Results demonstrate that beam propagation along the hohlraum wall is a push-broom process with complex dynamic spatial–temporal evolution.In the first few picoseconds,the optical intensity of the front position increases rapidly,while that of the rear position is relatively weak.The ratio R of the optical intensity during the rising edge is smaller than that of the steady state.R gradually increases and finally tends to the value of the steady state with time.Calculation also shows that,with shorter total width of the rising edge,R of the optical field decreases and the difference compared to the steady state becomes larger.The evolution is more severe with smaller angle of inclination.

  20. Fractographic analysis of tensile failures of aerospace grade composites

    Directory of Open Access Journals (Sweden)

    Masa Suresh Kumar

    2012-12-01

    Full Text Available This paper describes fractographic features observed in aerospace composites failed under tensile loads. Unidirectional Carbon Fibre Reinforced Plastic (UD CFRP and Unidirectional Glass Fibre Reinforced Plastic (UD GFRP composite specimens were fabricated and tested in tension. The morphology of fractured surfaces was studied at various locations to identify failure mechanism and characteristic fractographic features. CFRP composites displayed transverse crack propagation and the fracture surface showed three distinct regions, viz., crack origin, propagation and final failure. Significant variations in the fractographic features were noticed in crack propagation and final failure regions. Crack propagation region exhibited brittle fracture with chevron lines emanating from the crack origin. The entire crack propagation region exhibited radial marks on the individual fibre broken ends. On the other hand, the final fracture region revealed longitudinal matrix splitting and radial marks in majority of locations, and chop marks at some locations. The change in fracture mode in the final fracture was attributed to superimposition of bending loads. GFRP composites exhibited broom like fracture with extensive longitudinal splitting with radial marks present on individual fibre broken ends. Transverse fracture was observed at a few locations. These fracture features were analyzed and correlated with the loading conditions.

  1. High-resolution transcript profiling of the atypical biotrophic interaction between Theobroma cacao and the fungal pathogen Moniliophthora perniciosa.

    Science.gov (United States)

    Teixeira, Paulo José Pereira Lima; Thomazella, Daniela Paula de Toledo; Reis, Osvaldo; do Prado, Paula Favoretti Vital; do Rio, Maria Carolina Scatolin; Fiorin, Gabriel Lorencini; José, Juliana; Costa, Gustavo Gilson Lacerda; Negri, Victor Augusti; Mondego, Jorge Maurício Costa; Mieczkowski, Piotr; Pereira, Gonçalo Amarante Guimarães

    2014-11-01

    Witches' broom disease (WBD), caused by the hemibiotrophic fungus Moniliophthora perniciosa, is one of the most devastating diseases of Theobroma cacao, the chocolate tree. In contrast to other hemibiotrophic interactions, the WBD biotrophic stage lasts for months and is responsible for the most distinctive symptoms of the disease, which comprise drastic morphological changes in the infected shoots. Here, we used the dual RNA-seq approach to simultaneously assess the transcriptomes of cacao and M. perniciosa during their peculiar biotrophic interaction. Infection with M. perniciosa triggers massive metabolic reprogramming in the diseased tissues. Although apparently vigorous, the infected shoots are energetically expensive structures characterized by the induction of ineffective defense responses and by a clear carbon deprivation signature. Remarkably, the infection culminates in the establishment of a senescence process in the host, which signals the end of the WBD biotrophic stage. We analyzed the pathogen's transcriptome in unprecedented detail and thereby characterized the fungal nutritional and infection strategies during WBD and identified putative virulence effectors. Interestingly, M. perniciosa biotrophic mycelia develop as long-term parasites that orchestrate changes in plant metabolism to increase the availability of soluble nutrients before plant death. Collectively, our results provide unique insight into an intriguing tropical disease and advance our understanding of the development of (hemi)biotrophic plant-pathogen interactions.

  2. Novel receptor-like kinases in cacao contain PR-1 extracellular domains.

    Science.gov (United States)

    Teixeira, Paulo José Pereira Lima; Costa, Gustavo Gilson Lacerda; Fiorin, Gabriel Lorencini; Pereira, Gonçalo Amarante Guimarães; Mondego, Jorge Maurício Costa

    2013-08-01

    Members of the pathogenesis-related protein 1 (PR-1) family are well-known markers of plant defence responses, forming part of the arsenal of the secreted proteins produced on pathogen recognition. Here, we report the identification of two cacao (Theobroma cacao L.) PR-1s that are fused to transmembrane regions and serine/threonine kinase domains, in a manner characteristic of receptor-like kinases (RLKs). These proteins (TcPR-1f and TcPR-1g) were named PR-1 receptor kinases (PR-1RKs). Phylogenetic analysis of RLKs and PR-1 proteins from cacao indicated that PR-1RKs originated from a fusion between sequences encoding PR-1 and the kinase domain of a LecRLK (Lectin Receptor-Like Kinase). Retrotransposition marks surround TcPR-1f, suggesting that retrotransposition was involved in the origin of PR-1RKs. Genes with a similar domain architecture to cacao PR-1RKs were found in rice (Oryza sativa), barrel medic (Medicago truncatula) and a nonphototrophic bacterium (Herpetosiphon aurantiacus). However, their kinase domains differed from those found in LecRLKs, indicating the occurrence of convergent evolution. TcPR-1g expression was up-regulated in the biotrophic stage of witches' broom disease, suggesting a role for PR-1RKs during cacao defence responses. We hypothesize that PR-1RKs transduce a defence signal by interacting with a PR-1 ligand.

  3. The fungal pathogen Moniliophthora perniciosa has genes similar to plant PR-1 that are highly expressed during its interaction with cacao.

    Science.gov (United States)

    Teixeira, Paulo J P L; Thomazella, Daniela P T; Vidal, Ramon O; do Prado, Paula F V; Reis, Osvaldo; Baroni, Renata M; Franco, Sulamita F; Mieczkowski, Piotr; Pereira, Gonçalo A G; Mondego, Jorge M C

    2012-01-01

    The widespread SCP/TAPS superfamily (SCP/Tpx-1/Ag5/PR-1/Sc7) has multiple biological functions, including roles in the immune response of plants and animals, development of male reproductive tract in mammals, venom activity in insects and reptiles and host invasion by parasitic worms. Plant Pathogenesis Related 1 (PR-1) proteins belong to this superfamily and have been characterized as markers of induced defense against pathogens. This work presents the characterization of eleven genes homologous to plant PR-1 genes, designated as MpPR-1, which were identified in the genome of Moniliophthora perniciosa, a basidiomycete fungus responsible for causing the devastating witches' broom disease in cacao. We describe gene structure, protein alignment and modeling analyses of the MpPR-1 family. Additionally, the expression profiles of MpPR-1 genes were assessed by qPCR in different stages throughout the fungal life cycle. A specific expression pattern was verified for each member of the MpPR-1 family in the conditions analyzed. Interestingly, some of them were highly and specifically expressed during the interaction of the fungus with cacao, suggesting a role for the MpPR-1 proteins in the infective process of this pathogen. Hypothetical functions assigned to members of the MpPR-1 family include neutralization of plant defenses, antimicrobial activity to avoid competitors and fruiting body physiology. This study provides strong evidence on the importance of PR-1-like genes for fungal virulence on plants.

  4. FIRST REPORT OF 'CANDIDATUS PHYTOPLASMA ULMI' IN ULMUS LAEVIS IN GERMANY.

    Science.gov (United States)

    Eisold, A M; Kube, M; Holz, S; Büttner, C

    2015-01-01

    The wall-less bacteria of the provisory taxon 'Candidatus Phytoplasma' are obligate parasites and associated to diseases in many important crops and trees worldwide. 'Ca. Phytoplasma ulmi', assigned to 16SrV-A subgroup, is a quarantine pest and described to be associated to elm phloem necrosis, leaf yellowing, stunting, witches broom and decline in various elm species. Elm yellows phytoplasmas (EY) have been reported in several European countries but not in Ulmus laevis in Germany so far. Leaf samples from European white elms (Ulmus leavis PALL.) with and without chlorotic symptoms were investigated for EYs infection in Berlin and Brandenburg, Germany, through performing diagnostic nested PCR targeting partial rRNA operon of phytoplasmas. Specific PCR-products were obtained from 30 out of 59 samples. Partial 16S-rDNA sequences were assigned to 'Ca. P. ulmi' through sequence analysis, while sequence variation was observed. This is the first report of U. laevis infected with 'Ca. P. ulmi' in Germany.

  5. Hybrid Synthetic/Real Aperture Antenna for High Resolution Microwave Imaging

    Science.gov (United States)

    Doiron, Terence A.; Piepmeier, Jeffrey R.

    2003-01-01

    Observations of key hydrological parameters at the spatial and temporal scales required in the post-2002 era face significant technological challenges. These measurements are based on relatively low frequency thermal microwave emission (at 1.4 GHz for soil moisture and salinity, 10 GHz and up for precipitation, and 19 and 37 GHz for snow). The long wavelengths at these frequencies coupled with the high spatial and radiometric resolutions required by the various global hydrology missions necessitate the use of very large apertures. Two-dimensional Synthetic Thinned Array Radiometry (2-D STAR), though promising in the long term, has many technical challenges in the areas of power, and sensitivity for very large apertures (i.e. greater than 300 wavelengths). This paper will discuss an alternative approach to the pure 2-D STAR, which uses an offset parabolic cylinder reflector fed by multiple elements to form a 1-D STAR. In essence a single STAR element is composed of a feedhorn and parabolic cylinder reflector. The elements are sparsely arrayed and thus can share a single reflector. This antenna would have no moving parts once deployed, have much higher sensitivity than a Y-shaped 2-D STAR of equivalent size, many fewer receivers than that 2-D STAR, and the reflector could be made of a thin film and lightweight deployment system for high packing density. The instrument using this approach would be a cross track push broom imager. An overview of the design parameters, potential deployment mechanisms and applications will be presented.

  6. Proteomic analysis of the Mexican lime tree response to "Candidatus Phytoplasma aurantifolia" infection.

    Science.gov (United States)

    Taheri, Farzan; Nematzadeh, Ghorbanali; Zamharir, Maryam Ghayeb; Nekouei, Mojtaba Khayam; Naghavi, Mohammadreza; Mardi, Mohsen; Salekdeh, Ghasem Hosseini

    2011-11-01

    "Candidatus Phytoplasma aurantifolia" is the causative agent of witches' broom disease in the Mexican lime tree (Citrus aurantifolia L.), and is responsible for major tree losses in Southern Iran and Oman. The pathogen is strictly biotrophic, and, therefore, completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We applied a proteomics approach to analyse gene expression in Mexican limes infected with "Ca. Phytoplasma aurantifolia". Leaf samples were collected from healthy and infected plants and were analysed using 2-DE coupled with MS. Among 800 leaf proteins that were detected reproducibly in eight biological replicates of healthy and eight biological replicates of infected plants, 55 showed a significant response to the disease. MS resulted in identification of 39 regulated proteins, which included proteins that were involved in oxidative stress defence, photosynthesis, metabolism, and the stress response. Our results provide the first proteomic view of the molecular basis of the infection process and identify genes that could help inhibit the effects of the pathogen.

  7. Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees.

    Directory of Open Access Journals (Sweden)

    Farveh Ehya

    Full Text Available Witches' broom disease of Mexican lime (Citrus aurantifolia L., which is associated to the phytoplasma 'Candidatus Phytoplasma aurantifolia', is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing.Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with 'Ca. P. aurantifolia'.Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by 'Ca. P. aurantifolia'. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs, which are expressed differentially in response to pathogen infection.It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas.

  8. Real-time progressive hyperspectral remote sensing detection methods for crop pest and diseases

    Science.gov (United States)

    Wu, Taixia; Zhang, Lifu; Peng, Bo; Zhang, Hongming; Chen, Zhengfu; Gao, Min

    2016-05-01

    Crop pests and diseases is one of major agricultural disasters, which have caused heavy losses in agricultural production each year. Hyperspectral remote sensing technology is one of the most advanced and effective method for monitoring crop pests and diseases. However, Hyperspectral facing serial problems such as low degree of automation of data processing and poor timeliness of information extraction. It resulting we cannot respond quickly to crop pests and diseases in a critical period, and missed the best time for quantitative spraying control on a fixed point. In this study, we take the crop pests and diseases as research point and breakthrough, using a self-development line scanning VNIR field imaging spectrometer. Take the advantage of the progressive obtain image characteristics of the push-broom hyperspectral remote sensor, a synchronous real-time progressive hyperspectral algorithms and models will development. Namely, the object's information will get row by row just after the data obtained. It will greatly improve operating time and efficiency under the same detection accuracy. This may solve the poor timeliness problem when we using hyperspectral remote sensing for crop pests and diseases detection. Furthermore, this method will provide a common way for time-sensitive industrial applications, such as environment, disaster. It may providing methods and technical reserves for the development of real-time detection satellite technology.

  9. Cacao diseases: important threats to chocolate production worldwide.

    Science.gov (United States)

    Ploetz, Randy C

    2007-12-01

    ABSTRACT Theobroma cacao, cacao, is an ancient, neotropical domesticate. It is now grown throughout the humid, lowland tropics and is the basis of a multibillion dollar confectionary trade. Diverse diseases impact production of the crop. They reduce yields by ca. 20%, but could cause far greater losses if certain highly damaging diseases were to become more widely distributed. Among the most potentially dangerous of these diseases are frosty pod, caused by Moniliophthora roreri, and witches' broom, caused by M. perniciosa (previously Crinipellis perniciosa). These two diseases occur only in the Western Hemisphere, and severe losses would follow their introduction to West Africa and Asia, where ca. 86% of all cacao production occurs. Elsewhere, Cacao swollen shoot virus and the damaging black pod agent, Phytophthora megakarya, are found in Western Africa; whereas vascular streak dieback, caused by Oncobasidium theobromae, is present only in Asia. Breeding programs are challenged by minimal resistance to some of the diseases. Progress that has been made is threatened by the "emergence" of other serious diseases, such as Ceratocystis wilt (Ceratocystis cacaofunesta). During this symposium, new insights are discussed on the biology, origins, pathology and phylogeny of the pathogens; as well as the biological, chemical and genetic management of the diseases that they cause.

  10. Design of the infrared imaging chain for the PRISM hyperspectral imager

    Science.gov (United States)

    Dartois, Thierry; Giordanengo, Muriel; Ribet, Jean-Luc; Del Bello, Umberto

    2000-12-01

    ALCATEL has recently studied an infrared imaging chain, in the frame of phase A studies for the Land Surface Processes and Interactions Mission (LSPIM), which was one of the four candidate Core Missions for the European Space Agency Earth Explorer Program. The LSPIM satellite carries a single optical payload named PRISM (Processes Research by an Imaging Space Mission). PRISM is a multispectral imager based on the push broom imaging principle, operating at approximately 679 km altitude with a NADIR swath of 50 km associated to a 50 m spatial resolution. The paper presented herewith summarizes the results of the IR imaging chains study: composed of two IR focal planes (SWIR and TIR) integrated in dedicated ALCATEL dewars (one for each FPA), two proximity electronic modules and a common analog processing unit delivering digital video data to the one board mass memory unit (MMU). The main specifications of the detectors and electronic units are presented, a baseline of the imaging chain architecture complying with the requirements is then proposed with the main achieved trades off. The concept and associated performances of cutting-edge cooling systems are also introduced in this paper.

  11. Automatic Descriptor-Based Co-Registration of Frame Hyperspectral Data

    Directory of Open Access Journals (Sweden)

    Maria Vakalopoulou

    2014-04-01

    Full Text Available Frame hyperspectral sensors, in contrast to push-broom or line-scanning ones, produce hyperspectral datasets with, in general, better geometry but with unregistered spectral bands. Being acquired at different instances and due to platform motion and movements (UAVs, aircrafts, etc., every spectral band is displaced and acquired with a different geometry. The automatic and accurate registration of hyperspectral datasets from frame sensors remains a challenge. Powerful local feature descriptors when computed over the spectrum fail to extract enough correspondences and successfully complete the registration procedure. To this end, we propose a generic and automated framework which decomposes the problem and enables the efficient computation of a sufficient amount of accurate correspondences over the given spectrum, without using any ancillary data (e.g., from GPS/IMU. First, the spectral bands are divided in spectral groups according to their wavelength. The spectral borders of each group are not strict and their formulation allows certain overlaps. The spectral variance and proximity determine the applicability of every spectral band to act as a reference during the registration procedure. The proposed decomposition allows the descriptor and the robust estimation process to deliver numerous inliers. The search space of possible solutions has been effectively narrowed by sorting and selecting the optimal spectral bands which under an unsupervised manner can quickly recover hypercube’s geometry. The developed approach has been qualitatively and quantitatively evaluated with six different datasets obtained by frame sensors onboard aerial platforms and UAVs. Experimental results appear promising.

  12. Genetic testing of Korean familial hypercholesterolemia using whole-exome sequencing.

    Directory of Open Access Journals (Sweden)

    Soo Min Han

    Full Text Available Familial hypercholesterolemia (FH is a genetic disorder with an increased risk of early-onset coronary artery disease. Although some clinically diagnosed FH cases are caused by mutations in LDLR, APOB, or PCSK9, mutation detection rates and profiles can vary across ethnic groups. In this study, we aimed to provide insight into the spectrum of FH-causing mutations in Koreans. Among 136 patients referred for FH, 69 who met Simon Broome criteria with definite family history were enrolled. By whole-exome sequencing (WES analysis, we confirmed that the 3 known FH-related genes accounted for genetic causes in 23 patients (33.3%. A substantial portion of the mutations (19 of 23 patients, 82.6% resulted from 17 mutations and 2 copy number deletions in LDLR gene. Two mutations each in the APOB and PCSK9 genes were verified. Of these anomalies, two frameshift deletions in LDLR and one mutation in PCSK9 were identified as novel causative mutations. In particular, one novel mutation and copy number deletion were validated by co-segregation in their relatives. This study confirmed the utility of genetic diagnosis of FH through WES.

  13. 'Candidatus Phytoplasma lycopersici', a phytoplasma associated with 'hoja de perejil' disease in Bolivia.

    Science.gov (United States)

    Arocha, Yaima; Antesana, Olivia; Montellano, Ernesto; Franco, Pablo; Plata, G; Jones, Phil

    2007-08-01

    New diseases known locally as 'hoja de perejil' of tomato (Lycopersicon esculentum Mill) and 'brotes grandes' of potato (Solanum tuberosum L.) were first recognized in surveys of production fields in Bolivia during 2000-2003. Alfalfa (Medicago sativa) witches' broom and little leaf diseases of native weeds Morrenia variegata and mora-mora (Serjania perulacea) were also identified near to production fields. Phytoplasma aetiology was attributed to each of these diseases following detection and initial identification of aster yellows group (16SrI) phytoplasmas in all five diseased plant species. While potato, alfalfa and mora-mora plants contained indistinguishable 16SrI-B strains, 'hoja de perejil' (THP) and morrenia little leaf (MVLL)-associated phytoplasma strains shared 97.5 % 16S rRNA gene sequence similarity with 'Candidatus Phytoplasma asteris' and related strains and <95 % similarity with all other 'Candidatus Phytoplasma' species. Phylogenetic analysis of 16S rRNA gene sequences indicated that the THP and MVLL phytoplasmas represent a novel lineage within the aster yellows (16SrI) group and, on the basis of unique 16S rRNA gene sequences, we propose that THP and MVLL phytoplasmas represent 'Candidatus Phytoplasma lycopersici', with THP as the reference strain.

  14. Molecules and Morphology Reveal Overlooked Populations of Two Presumed Extinct Australian Sea Snakes (Aipysurus: Hydrophiinae)

    Science.gov (United States)

    Sanders, Kate L.; Schroeder, Tina; Guinea, Michael L.; Rasmussen, Arne R.

    2015-01-01

    The critically endangered leaf-scaled (Aipysurus foliosquamaI) and short-nosed (A. apraefrontalis) sea snakes are currently recognised only from Ashmore and Hibernia reefs ~600km off the northwest Australian coast. Steep population declines in both species were documented over 15 years and neither has been sighted on dedicated surveys of Ashmore and Hibernia since 2001. We examine specimens of these species that were collected from coastal northwest Australian habitats up until 2010 (A.foliosquama) and 2012 (A. apraefrontalis) and were either overlooked or treated as vagrants in conservation assessments. Morphological variation and mitochondrial sequence data confirm the assignment of these coastal specimens to A. foliosquama (Barrow Island, and offshore from Port Hedland) and A.apraefrontalis (Exmouth Gulf, and offshore from Roebourne and Broome). Collection dates, and molecular and morphological variation between coastal and offshore specimens, suggest that the coastal specimens are not vagrants as previously suspected, but instead represent separate breeding populations. The newly recognised populations present another chance for leaf-scaled and short-nosed sea snakes, but coastal habitats in northwest Australia are widely threatened by infrastructure developments and sea snakes are presently omitted from environmental impact assessments for industry. Further studies are urgently needed to assess these species’ remaining distributions, population structure, and extent of occurrence in protected areas. PMID:25671608

  15. A preliminary result of self-calibration bundle adjustment of Chang'E-2 stereo imagery

    Science.gov (United States)

    Liu, Yiliang; Liu, Bin; Peng, Man; Di, Kaichang

    2014-05-01

    The high resolution lunar global images acquired by Chang'E-2 (CE-2) CCD camera orbiter are of great importance for lunar science research as well as preparation of the landing and surface operation of Chang'E-3 (CE-3) lunar rover. In this paper, a rigorous geometric model of CE-2 CCD camera is developed based on the push-broom imaging principle. A self-calibration bundle adjustment (SCBA) method is proposed to eliminate the inconsistencies between forward- and backward-looking images, which are caused by the inaccuracy of exterior orientation (EO) parameters and the uncertain relationship between the two CCD line arrays of the camera. The interior orientation (IO) model is refined by adding several additional parameters and the EO parameters are fitted by a third-order polynomial model. Strategies for ensuring the robustness and reliability of the solution are also adopted, including EO pseudo observations selection, reasonable weight determination, and truncated singular value decomposition method. After adjustment, the inconsistencies between the forward- and backward-looking images are eliminated effectively by reducing the image-space residuals from around 20 pixels to sub-pixel. Based on the adjustment, high precision DEM (Digital Elevation Model) and DOM (Digital Ortho Map) of a local area at Sinus Iridum (pre-selected CE-3 landing site) are generated automatically.

  16. Isolation and purification of functional total RNA from different organs of cacao tree during its interaction with the pathogen Crinipellis perniciosa.

    Science.gov (United States)

    Gesteira, Abelmon da Silva; Micheli, Fabienne; Ferreira, Cláudia Fortes; Cascardo, Júlio Cézar de Mattos

    2003-09-01

    Witches' broom disease, caused by Crinipellis perniciosa, is one of the major fungal diseases causing severe losses to cacao tree (Theobroma cacao L.) plantations in South America. One of the challenges associated with the understanding of the cacao and Crinipellis interaction in genomic studies is the isolation of intact nucleic acids. In this report, we describe a new, successful, and reliable procedure for the isolation of RNA from tissues of cacao tree, both infected and uninfected by Crinipellis. This protocol overcomes the problems associated with the very high amount of polyphenols and polysaccharides present in cacao organs that are not easily removed by conventional extraction procedures. The protocol requires few reagents, uses ultracentrifugation and inexpensive consumables, and can be easily applied in any laboratory. This method produced high-quality RNA that was suitable for subsequent purposes, such as reverse transcription PCR and cDNA library construction. We also report the first evidence of RNA isolation from cacao organs infected by C. perniciosa such as meristems and fruits.

  17. Plant-Pathogen Interaction-Related MicroRNAs and Their Targets Provide Indicators of Phytoplasma Infection in Paulownia tomentosa × Paulownia fortunei.

    Science.gov (United States)

    Fan, Guoqiang; Niu, Suyan; Xu, Tong; Deng, Minjie; Zhao, Zhenli; Wang, Yuanlong; Cao, Lin; Wang, Zhe

    2015-01-01

    Paulownia witches' broom (PaWB) caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L-1 and 30 mg·L-1 methyl methane sulfonate (MMS)-treated plants. In total, 125 conserved and 118 novel microRNAs (miRNAs) were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in plant-pathogen interaction and plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand plant-phytoplasma interactions.

  18. Plant-Pathogen Interaction-Related MicroRNAs and Their Targets Provide Indicators of Phytoplasma Infection in Paulownia tomentosa × Paulownia fortunei.

    Directory of Open Access Journals (Sweden)

    Guoqiang Fan

    Full Text Available Paulownia witches' broom (PaWB caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L-1 and 30 mg·L-1 methyl methane sulfonate (MMS-treated plants. In total, 125 conserved and 118 novel microRNAs (miRNAs were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in plant-pathogen interaction and plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand plant-phytoplasma interactions.

  19. Plant–Pathogen Interaction-Related MicroRNAs and Their Targets Provide Indicators of Phytoplasma Infection in Paulownia tomentosa × Paulownia fortunei

    Science.gov (United States)

    Fan, Guoqiang; Niu, Suyan; Xu, Tong; Deng, Minjie; Zhao, Zhenli; Wang, Yuanlong; Cao, Lin; Wang, Zhe

    2015-01-01

    Paulownia witches’ broom (PaWB) caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L−1 and 30 mg·L−1 methyl methane sulfonate (MMS)-treated plants. In total, 125 conserved and 118 novel microRNAs (miRNAs) were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in plant-pathogen interaction and plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand plant-phytoplasma interactions. PMID:26484670

  20. Special Software for Planetary Image Processing and Research

    Science.gov (United States)

    Zubarev, A. E.; Nadezhdina, I. E.; Kozlova, N. A.; Brusnikin, E. S.; Karachevtseva, I. P.

    2016-06-01

    The special modules of photogrammetric processing of remote sensing data that provide the opportunity to effectively organize and optimize the planetary studies were developed. As basic application the commercial software package PHOTOMOD™ is used. Special modules were created to perform various types of data processing: calculation of preliminary navigation parameters, calculation of shape parameters of celestial body, global view image orthorectification, estimation of Sun illumination and Earth visibilities from planetary surface. For photogrammetric processing the different types of data have been used, including images of the Moon, Mars, Mercury, Phobos, Galilean satellites and Enceladus obtained by frame or push-broom cameras. We used modern planetary data and images that were taken over the years, shooting from orbit flight path with various illumination and resolution as well as obtained by planetary rovers from surface. Planetary data image processing is a complex task, and as usual it can take from few months to years. We present our efficient pipeline procedure that provides the possibilities to obtain different data products and supports a long way from planetary images to celestial body maps. The obtained data - new three-dimensional control point networks, elevation models, orthomosaics - provided accurate maps production: a new Phobos atlas (Karachevtseva et al., 2015) and various thematic maps that derived from studies of planetary surface (Karachevtseva et al., 2016a).

  1. Multiplex-PCR for Identification of Two Species in Genus Hishimonus (Hemiptera: Cicadellidae) in Jujube Orchards.

    Science.gov (United States)

    Hao, Shaodong; Wang, He; Tao, Wanqiang; Wang, Jinzhong; Zhang, Zhiyong; Zhang, Qiuling; Zhang, Minzhao; Guo, Li; Shi, Xiaoyu

    2015-10-01

    The insect family Cicadellidae includes economically important vectors of plant pathogens. Hishimonus sellatus (Uhler) transmits jujube witches'-broom (JWB). Currently, H. sellatus and Hishimonus lamellatus Cai et Kuoh are observed to co-occur at the same locality on jujube. H. lamellatus is now suspected to be a JWB vector. As such, correct identification of Hishimonus species present in vineyards is essential for epidemiological surveys. However, traditional identification of Hishimonus by morphology is limited to the adult male. We provide a comprehensive description of morphological and molecular tools for discriminating between H. sellatus and H. lamellatus, for use in identification and monitoring of the two Hishimonus species and studies of their plant hosts. A rapid and inexpensive method is introduced to identify H. sellatus and H. lamellatus occurring in jujube orchards. This method is based on amplification of mitochondrial cytochrome oxidase I (COI) gene, using PCR with multiplexed, species-specific primers. The reliability of this new method has been tested on different populations from different sites in Beijing region of China.

  2. A Pilot Study on Integrating Videography and Environmental Microbial Sampling to Model Fecal Bacterial Exposures in Peri-Urban Tanzania.

    Directory of Open Access Journals (Sweden)

    Timothy R Julian

    Full Text Available Diarrheal diseases are a leading cause of under-five mortality and morbidity in sub-Saharan Africa. Quantitative exposure modeling provides opportunities to investigate the relative importance of fecal-oral transmission routes (e.g. hands, water, food responsible for diarrheal disease. Modeling, however, requires accurate descriptions of individuals' interactions with the environment (i.e., activity data. Such activity data are largely lacking for people in low-income settings. In the present study, we collected activity data and microbiological sampling data to develop a quantitative microbial exposure model for two female caretakers in peri-urban Tanzania. Activity data were combined with microbiological data of contacted surfaces and fomites (e.g. broom handle, soil, clothing to develop example exposure profiles describing second-by-second estimates of fecal indicator bacteria (E. coli and enterococci concentrations on the caretaker's hands. The study demonstrates the application and utility of video activity data to quantify exposure factors for people in low-income countries and apply these factors to understand fecal contamination exposure pathways. This study provides both a methodological approach for the design and implementation of larger studies, and preliminary data suggesting contacts with dirt and sand may be important mechanisms of hand contamination. Increasing the scale of activity data collection and modeling to investigate individual-level exposure profiles within target populations for specific exposure scenarios would provide opportunities to identify the relative importance of fecal-oral disease transmission routes.

  3. A new design approach to innovative spectrometers. Case study: TROPOLITE

    Science.gov (United States)

    Volatier, Jean-Baptiste; Baümer, Stefan; Kruizinga, Bob; Vink, Rob

    2014-05-01

    Designing a novel optical system is a nested iterative process. The optimization loop, from a starting point to final system is already mostly automated. However this loop is part of a wider loop which is not. This wider loop starts with an optical specification and ends with a manufacturability assessment. When designing a new spectrometer with emphasis on weight and cost, numerous iterations between the optical- and mechanical designer are inevitable. The optical designer must then be able to reliably produce optical designs based on new input gained from multidisciplinary studies. This paper presents a procedure that can automatically generate new starting points based on any kind of input or new constraint that might arise. These starting points can then be handed over to a generic optimization routine to make the design tasks extremely efficient. The optical designer job is then not to design optical systems, but to meta-design a procedure that produces optical systems paving the way for system level optimization. We present here this procedure and its application to the design of TROPOLITE a lightweight push broom imaging spectrometer.

  4. Determination of technical readiness for an atmospheric carbon imaging spectrometer

    Science.gov (United States)

    Mobilia, Joseph; Kumer, John B.; Palmer, Alice; Sawyer, Kevin; Mao, Yalan; Katz, Noah; Mix, Jack; Nast, Ted; Clark, Charles S.; Vanbezooijen, Roel; Magoncelli, Antonio; Baraze, Ronald A.; Chenette, David L.

    2013-09-01

    The geoCARB sensor uses a 4-channel push broom slit-scan infrared imaging grating spectrometer to measure the absorption spectra of sunlight reflected from the ground in narrow wavelength regions. The instrument is designed for flight at geostationary orbit to provide mapping of greenhouse gases over continental scales, several times per day, with a spatial resolution of a few kilometers. The sensor provides multiple daily maps of column-averaged mixing ratios of CO2, CH4, and CO over the regions of interest, which enables flux determination at unprecedented time, space, and accuracy scales. The geoCARB sensor development is based on our experience in successful implementation of advanced space deployed optical instruments for remote sensing. A few recent examples include the Atmospheric Imaging Assembly (AIA) and Helioseismic and Magnetic Imager (HMI) on the geostationary Solar Dynamics Observatory (SDO), the Space Based Infrared System (SBIRS GEO-1) and the Interface Region Imaging Spectrograph (IRIS), along with sensors under development, the Near Infared camera (NIRCam) for James Webb (JWST), and the Global Lightning Mapper (GLM) and Solar UltraViolet Imager (SUVI) for the GOES-R series. The Tropospheric Infrared Mapping Spectrometer (TIMS), developed in part through the NASA Instrument Incubator Program (IIP), provides an important part of the strong technological foundation for geoCARB. The paper discusses subsystem heritage and technology readiness levels for these subsystems. The system level flight technology readiness and methods used to determine this level are presented along with plans to enhance the level.

  5. Effect of different cultivating factors on potato yield%不同栽培因素对马铃薯产量的影响

    Institute of Scientific and Technical Information of China (English)

    邱荣彬; 李广昌; 陈锐芬

    2012-01-01

    研究基肥施用方法、种薯切块大小和膨大素喷施次数对马铃薯生长、产量及品质的影响,试验结果表明:每667 m2用鸡粪375 kg+复合肥25 kg、种薯切块30 g、膨大素喷施2次(现蕾期和盛花期各1次)处理效果最优。%Effects of basal fertilizer-applying methods,cutting size of potato seedtuber and spraying times of forchlorfenuron(CPPU) on the growth,tuber yield and quality of potato were studied.The results showed that the best treating efficiency was found by applying chicken waste 375 kg/667m2+compound fertilizers 25 kg/667m2,using the size of 30 g potato seedtuber,and spraying two times of CPPU(at squaring period and full broom period,respectively).

  6. Development in nursery of different clones of cupuaçu tree (Theobroma grandiflorum (Wild. Ex Spreng. Schum

    Directory of Open Access Journals (Sweden)

    D. Franciskievicz

    2013-11-01

    Full Text Available This work aimed to present the results of initial development in nursery of 4 genetic materials of cupuaçu trees, being one a material without selection (control and three clones of cupuaçu tree resistant to the fungus Moniliophthora perniciosa, causal agent of Witches' Broom. The seeds from Embrapa Amazônia Oriental (Belém - PA were put in seed bed for germination. After 25 days of seedling emergence these were transplanted in to plastic bags, arranged in a shade house (60%, and containing agricultural substrate and forest humus (1: 3. At the end of 130 days after sowing were compared the patterns of development of girth, height and relation height / girth in a randomized block design consisting of 4 treatments and 4 replicates, each replicate consisting of 16 seedlings. Results were subjected to analysis of variance (ANOVA and the averages compared by Tukey (5%. The values ​​obtained to girth not differ, presented as overall mean 5.08 mm, however in relation to height cultivar 215 (31cm excelled on the cultivar 174 (25 cm and control (23 cm, however their average not differ of material 186 (27 cm. As regards the height / girth the values ​​obtained for the control and cultivars 175, 186 and 215, were respectively, 4.6, 5.2, 5.1 and 5.9 cm / mm, being cultivar 215 different to control.

  7. Cultural Explanations of Sleep Paralysis in Italy: The Pandafeche Attack and Associated Supernatural Beliefs.

    Science.gov (United States)

    Jalal, Baland; Romanelli, Andrea; Hinton, Devon E

    2015-12-01

    The current study examines cultural explanations regarding sleep paralysis (SP) in Italy. The study explores (1) whether the phenomenology of SP generates culturally specific interpretations and causal explanations and (2) what are the beliefs and local traditions associated with such cultural explanations. The participants were Italian nationals from the general population (n = 68) recruited in the region of Abruzzo, Italy. All participants had experienced at least one lifetime episode of SP. The sleep paralysis experiences and phenomenology questionnaire were orally administered to participants. We found a multilayered cultural interpretation of SP, namely the Pandafeche attack, associated with various supernatural beliefs. Thirty-eight percent of participants believed that this supernatural being, the Pandafeche-often referred to as an evil witch, sometimes as a ghost-like spirit or a terrifying humanoid cat-might have caused their SP. Twenty-four percent of all participants sensed the Pandafeche was present during their SP. Strategies to prevent Pandafeche attack included sleeping in supine position, placing a broom by the bedroom door, or putting a pile of sand by the bed. Case studies are presented to illustrate the study findings. The Pandafeche attack thus constitutes a culturally specific, supernatural interpretation of the phenomenology of SP in the Abruzzo region of Italy.

  8. Comparison of Efficacy in Abnormal Cervical Cell Detection between Liquid-based Cytology and Conventional Cytology.

    Science.gov (United States)

    Tanabodee, Jitraporn; Thepsuwan, Kitisak; Karalak, Anant; Laoaree, Orawan; Krachang, Anong; Manmatt, Kittipong; Anontwatanawong, Nualpan

    2015-01-01

    This study was conducted to 1206 women who had cervical cancer screening at Chonburi Cancer Hospital. The spilt-sample study aimed to compare the efficacy of abnormal cervical cells detection between liquid-based cytology (LBC) and conventional cytology (CC). The collection of cervical cells was performed by broom and directly smeared on a glass slide for CC then the rest of specimen was prepared for LBC. All slides were evaluated and classified by The Bethesda System. The results of the two cytological tests were compared to the gold standard. The LBC smear significantly decreased inflammatory cell and thick smear on slides. These two techniques were not difference in detection rate of abnormal cytology and had high cytological diagnostic agreement of 95.7%. The histologic diagnosis of cervical tissue was used as the gold standard in 103 cases. Sensitivity, specificity, positive predictive value, negative predictive value, false positive, false negative and accuracy of LBC at ASC-US cut off were 81.4, 75.0, 70.0, 84.9, 25.0, 18.6 and 77.7%, respectively. CC had higher false positive and false negative than LBC. LBC had shown higher sensitivity, specificity, PPV, NPV and accuracy than CC but no statistical significance. In conclusion, LBC method can improve specimen quality, more sensitive, specific and accurate at ASC-US cut off and as effective as CC in detecting cervical epithelial cell abnormalities.

  9. Bevalac operations update

    Energy Technology Data Exchange (ETDEWEB)

    1991-02-01

    The Bevalac passed the recent Tiger Team assessment with flying colors, thanks to the unremitting effort of countless people over the last several months, pulling miles of cables, sending tons of valuable'' junk to salvage, painting, pushing brooms, writing operating procedures. It is time to look ahead now and plan for doing science again; however, it is not business as usual. The Bevalac survived, but not without changes. There are more procedures to be followed, safety appraisals to be made, training to be gone through. The primary goal is not just to get a task done, but to get it done in a safe manner according to code. If this means a delay in a run because enough time wasn't allotted for making a change in the setup, then the experiment will be delayed. Many of the obvious changes you will find the next time you come to work here are summarized in this newsletter. We will inform you of others as they are forthcoming. Also we will discuss these changes at our We Survived the Tigers, but{hor ellipsis}'' Users' Information Meeting at the Washington APS meeting. Details are inside this report.

  10. Terrain mapping camera for Chandrayaan-1

    Indian Academy of Sciences (India)

    A S Kiran Kumar; A Roy Chowdhury

    2005-12-01

    The Terrain Mapping Camera (TMC)on India ’s first satellite for lunar exploration,Chandrayaan-1, is for generating high-resolution 3-dimensional maps of the Moon.With this instrument,a complete topographic map of the Moon with 5 m spatial resolution and 10-bit quantization will be available for scienti fic studies.The TMC will image within the panchromatic spectral band of 0.4 to 0.9 m with a stereo view in the fore,nadir and aft directions of the spacecraft movement and have a B/H ratio of 1.The swath coverage will be 20 km.The camera is configured for imaging in the push broom-mode with three linear detectors in the image plane.The camera will have four gain settings to cover the varying illumination conditions of the Moon.Additionally,a provision of imaging with reduced resolution,for improving Signal-to-Noise Ratio (SNR)in polar regions,which have poor illumination conditions throughout,has been made.SNR of better than 100 is expected in the ± 60° latitude region for mature mare soil,which is one of the darkest regions on the lunar surface. This paper presents a brief description of the TMC instrument.

  11. Expression of an oxalate decarboxylase impairs the necrotic effect induced by Nep1-like protein (NLP) of Moniliophthora perniciosa in transgenic tobacco.

    Science.gov (United States)

    da Silva, Leonardo F; Dias, Cristiano V; Cidade, Luciana C; Mendes, Juliano S; Pirovani, Carlos P; Alvim, Fátima C; Pereira, Gonçalo A G; Aragão, Francisco J L; Cascardo, Júlio C M; Costa, Marcio G C

    2011-07-01

    Oxalic acid (OA) and Nep1-like proteins (NLP) are recognized as elicitors of programmed cell death (PCD) in plants, which is crucial for the pathogenic success of necrotrophic plant pathogens and involves reactive oxygen species (ROS). To determine the importance of oxalate as a source of ROS for OA- and NLP-induced cell death, a full-length cDNA coding for an oxalate decarboxylase (FvOXDC) from the basidiomycete Flammulina velutipes, which converts OA into CO(2) and formate, was overexpressed in tobacco plants. The transgenic plants contained less OA and more formic acid compared with the control plants and showed enhanced resistance to cell death induced by exogenous OA and MpNEP2, an NLP of the hemibiotrophic fungus Moniliophthora perniciosa. This resistance was correlated with the inhibition of ROS formation in the transgenic plants inoculated with OA, MpNEP2, or a combination of both PCD elicitors. Taken together, these results have established a pivotal function for oxalate as a source of ROS required for the PCD-inducing activity of OA and NLP. The results also indicate that FvOXDC represents a potentially novel source of resistance against OA- and NLP-producing pathogens such as M. perniciosa, the causal agent of witches' broom disease of cacao (Theobroma cacao L.).

  12. Lessons Learned From Community-Based Approaches to Sodium Reduction

    Science.gov (United States)

    Kane, Heather; Strazza, Karen; Losby PhD, Jan L.; Lane, Rashon; Mugavero, Kristy; Anater, Andrea S.; Frost, Corey; Margolis, Marjorie; Hersey, James

    2017-01-01

    Purpose This article describes lessons from a Centers for Disease Control and Prevention initiative encompassing sodium reduction interventions in six communities. Design A multiple case study design was used. Setting This evaluation examined data from programs implemented in six communities located in New York (Broome County, Schenectady County, and New York City); California (Los Angeles County and Shasta County); and Kansas (Shawnee County). Subjects Participants (n = 80) included program staff, program directors, state-level staff, and partners. Measures Measures for this evaluation included challenges, facilitators, and lessons learned from implementing sodium reduction strategies. Analysis The project team conducted a document review of program materials and semi structured interviews 12 to 14 months after implementation. The team coded and analyzed data deductively and inductively. Results Five lessons for implementing community-based sodium reduction approaches emerged: (1) build relationships with partners to understand their concerns, (2) involve individuals knowledgeable about specific venues early, (3) incorporate sodium reduction efforts and messaging into broader nutrition efforts, (4) design the program to reduce sodium gradually to take into account consumer preferences and taste transitions, and (5) identify ways to address the cost of lower-sodium products. Conclusion The experiences of the six communities may assist practitioners in planning community-based sodium reduction interventions. Addressing sodium reduction using a community-based approach can foster meaningful change in dietary sodium consumption. PMID:24575726

  13. Effectiveness of long-term (twelve months nonsurgical weight loss interventions for obese women with polycystic ovary syndrome: a systematic review

    Directory of Open Access Journals (Sweden)

    Fiona Nicholson

    2010-11-01

    Full Text Available Fiona Nicholson1, Catherine Rolland1, John Broom1, John Love21Centre for Obesity Research and Epidemiology, Robert Gordon University, Aberdeen, Scotland; 2School of Applied Social Studies, Faculty of Health and Social Care, The Robert Gordon University, Aberdeen, ScotlandAbstract: Polycystic ovary syndrome (PCOS affects 2%–26% of women of reproductive age and is often accompanied by obesity. Modest weight loss reduces health risks and ameliorates effects of the syndrome. Weight loss interventions are mainly of short duration and have limited success. A systematic review of the literature was carried out to assess the efficacy of long-term (12 months, nonsurgical weight loss interventions for women with PCOS. Fifteen databases were searched, resulting in eight papers that met the search criteria. Comparison of results and meta-analysis was difficult due to heterogeneity of studies. Behavioral components of interventions were poorly described, and compliance was difficult to ascertain. The results suggested that the inclusion of a lifestyle component improves outcomes, but protocols must be clearly described to maintain study validity and to identify successful behavioral strategies.Keywords: obesity, polycystic ovary syndrome, weight loss 

  14. Infanticide risk and infant defence in multi-male free-ranging sooty mangabeys, Cercocebus atys.

    Science.gov (United States)

    Fruteau, Cécile; Range, Friederike; Noë, Ronald

    2010-01-01

    For years, infanticide by males was thought to be unlikely in multi-male primate species. Recent studies have, however, presented evidence of infanticide in such species and a recent model by Broom and colleagues predicts that males' age and rank influence the occurrence of infanticide: youngest and highest-ranking immigrant males are more likely to commit infanticide than their older and lower-ranking counterparts if putative fathers fail to protect infants. I collected data on adult free-ranging sooty mangabey females in the Taï National Park, Ivory Coast, over 11 months including a birth and a mating season. Infanticide had been previously reported in captivity for this species, but not in the wild. Several males entered the group prior to and during the mating season. As predicted by the model, only the more dominant immigrant ones attacked mother-infant pairs significantly more often than did other males. Mothers often reacted with counter-attacks. Potential fathers guarded and supported infants and mothers throughout the period of infant vulnerability. Furthermore, as only one of seven infants died despite 136 observed attacks on mother-infant pairs and unattended infants by immigrant males, we conclude that cooperation between putative fathers and mothers represents an effective protection against infanticide.

  15. Research on the trajectory model for ZY-3.

    Science.gov (United States)

    Chen, Yifu; Xie, Zhong

    2014-01-01

    The new generation Chinese high-resolution three-line stereo-mapping satellite Ziyuan 3 (ZY-3) is equipped with three sensors (nadir, backward, and forward views). Its objective is to manufacture the 1 : 50000 topographic map and revise and update the 1 : 25000 topographic map. For the push-broom satellite, the interpolation accuracy of orbit and attitude determines directly the satellite's stereo-mapping accuracy and the position accuracy without ground control point. In this study, a new trajectory model is proposed for ZY-3 in this paper, according to researching and analyzing the orbit and attitude of ZY-3. Using the trajectory data set, the correction and accuracy of the new proposed trajectory are validated and compared with the other models, polynomial model (LPM), piecewise polynomial model (PPM), and Lagrange cubic polynomial model (LCPM). Meanwhile, the differential equation is derivate for the bundle block adjustment. Finally, the correction and practicability of piece-point with weight polynomial model for ZY-3 satellite are validated according to the experiment of geometric correction using the ZY-3 image and orbit and attitude data.

  16. Research on the Trajectory Model for ZY-3

    Directory of Open Access Journals (Sweden)

    Yifu Chen

    2014-01-01

    Full Text Available The new generation Chinese high-resolution three-line stereo-mapping satellite Ziyuan 3 (ZY-3 is equipped with three sensors (nadir, backward, and forward views. Its objective is to manufacture the 1 : 50000 topographic map and revise and update the 1 : 25000 topographic map. For the push-broom satellite, the interpolation accuracy of orbit and attitude determines directly the satellite’s stereo-mapping accuracy and the position accuracy without ground control point. In this study, a new trajectory model is proposed for ZY-3 in this paper, according to researching and analyzing the orbit and attitude of ZY-3. Using the trajectory data set, the correction and accuracy of the new proposed trajectory are validated and compared with the other models, polynomial model (LPM, piecewise polynomial model (PPM, and Lagrange cubic polynomial model (LCPM. Meanwhile, the differential equation is derivate for the bundle block adjustment. Finally, the correction and practicability of piece-point with weight polynomial model for ZY-3 satellite are validated according to the experiment of geometric correction using the ZY-3 image and orbit and attitude data.

  17. Agricultural, domestic and handicraft folk uses of plants in the Tyrrhenian sector of Basilicata (Italy

    Directory of Open Access Journals (Sweden)

    Guarrera Paolo

    2005-07-01

    Full Text Available Abstract Background Research was carried out into agricultural and domestic-handicraft uses in folk traditions in the Tyrrhenian sector of the Basilicata region (southern Italy, as it is typically representative of ethnobotanical applications in the Mediterranean area. From the point of view of furnishing a botanical support for the study of local "material culture" data was collected through field interviews of 49 informants, most of whom were farmers. Results The taxa cited are 60, belonging to 32 botanical families, of which 18 are employed for agricultural uses and 51 for domestic-handicraft folk uses. Data show a diffuse use of plants for many purposes, both in agricultural (present uses 14%; past uses 1% and for domestic-handicraft use (present uses 40%; past uses 45%; most of the latter are now in decline. Conclusion 60 data look uncommon or typical of the places studied. Some domestic-handicraft folk uses are typical of southern Italy (e.g. the use of Ampelodesmos mauritanicus for making ties, ropes, torches, baskets or that of Acer neapolitanum for several uses. Other uses (e.g. that of Inula viscosa and Calamintha nepeta for peculiar brooms, and of Origanum heracleoticum for dyeing wool red are previously unpublished.

  18. Farmers' perceptions, knowledge, and management of coffee pests and diseases and their natural enemies in Chiapas, Mexico.

    Science.gov (United States)

    Segura, H R; Barrera, J F; Morales, H; Nazar, A

    2004-10-01

    Small farmers' perceptions of coffee Coffea arabica L. herbivores and their natural enemies, how those perceptions relate to field infestation levels, and pest management practices being implemented by members from two organic and nonorganic coffee grower organizations in the Soconusco region, southeastern Mexico, were analyzed through an interview survey, diagnostic workshops, and field sampling. The terms pest, disease, and damage were commonly used as synonyms. The major phytophagous species, as perceived by the interviewees, were Hypothenemus hampei (Ferrari), and to a lesser extent the fungi Corticium koleroga Cooke (Höhnel) and Hemileia vastatrix Berkeley & Broome. Among the nonorganic farmers, other nonpest-related constraints were regarded as more important. Awareness of the existence of natural enemies was low, despite more organic farmers have used the ectoparasitoid bethylid Cephalonomia stephanoderis Betrem against H. hampei. Labor supplied by household members was most frequent for pest control; only organic farmers exchanged labor for this purpose. The levels of infestation by H. hampei, Leucoptera coffeella Guérin-Méneville, and C. koleroga were lower within the organic coffee stands. However, a low effectiveness for pest control was commonly perceived, probably due to a feeling, among the organic farmers, of a low impact of their pest management extension service, whereas a lack of motivation was prevalent among the nonorganic farmers, shown by a concern with their low coffee yields and the emigration of youth. The importance of understanding farmers' perceptions and knowledge of pests and their natural enemies and the need for participatory pest management approaches, are discussed.

  19. Ricoseius loxocheles, a phytoseiid mite that feeds on coffee leaf rust.

    Science.gov (United States)

    Oliveira, Cleber M; Ferreira, João A M; Oliveira, Rafael M; Santos, Francisco O; Pallini, Angelo

    2014-10-01

    One of the most important diseases of coffee plants is the coffee leaf rust fungus Hemileia vastatrix Berkeley and Broome (Uredinales). It can cause 30 % yield loss in some varieties of Coffea arabica (L.). Besides fungus, the coffee plants are attacked by phytophagous mites. The most common species is the coffee red mite, Oligonychus ilicis McGregor (Acari: Tetranychidae). Predatory mites of the Phytoseiidae family are well-known for their potential to control herbivorous mites and insects, but they can also develop and reproduce on various other food sources, such as plant pathogenic fungi. In a field survey, we found Ricoseius loxocheles (De Leon) (Acari: Phytoseiidae) on the necrotic areas caused by the coffee leaf rust fungus during the reproductive phase of the pathogen. We therefore assessed the development, survivorship and reproduction of R. loxocheles feeding on coffee leaf rust fungus and measured predation and oviposition of this phytoseiid having coffee red mite as prey under laboratory conditions. The mite fed, survived, developed and reproduced successfully on this pathogen but it was not able to prey on O. ilicis. Survival and oviposition with only prey were the same as without food. This phytoseiid mite does not really use O. ilicis as food. It is suggested that R. loxocheles is one phytoseiid that uses fungi as a main food source.

  20. Edinburgh, the Scottish pioneers of anatomy and their lasting influence in South Africa.

    Science.gov (United States)

    Correia, J C; Wessels, Q; Vorster, W

    2013-11-01

    The history of the origin of anatomy education in South Africa is the history of an arduous journey through time. The lasting influence of Edinburgh came in the form of Robert Black Thomson. He was a student and assistant of Sir William Turner who gave rise to the first chair of anatomy and the establishment of a department at the South African College, known today as University of Cape Town. Thomson was later succeeded by Matthew Drennan, a keen anthropologist, who was revered by his students. This Scottish link prevailed over time with the appointment of Edward Philip Stibbe as the chair of anatomy at the South African School of Mines and Technology, which later became the University of the Witwatersrand. Stibbe's successor, Raymond Arthur Dart, a graduate of the University of Sydney, was trained in an anatomy department sculpted on that of Edinburgh by Professor James Thomas Wilson. Wilson's influence at the University of Sydney can be traced back to Edinburgh and William Turner through Thomas Anderson Stuart. Both Dart and Robert Broom, another Scot, were considered as Africa's wild men by the late Professor Tobias. Here, the authors explore the Scottish link and origins of anatomy pedagogy in South Africa.

  1. Narrow band SWIR hyperspectral imaging: a new approach based on volume Bragg grating

    Science.gov (United States)

    Verhaegen, M.; Lessard, S.; Blais-Ouellette, S.

    2012-06-01

    Volume Bragg grating technology has enabled the development of a new type of staring hyperspectral camera. Based on Bragg Tunable filters, these hyperspectral cameras have both high spectral and spatial resolution, and significantly higher sensitivity than competing technologies like push broom spectrometer, liquid crystal tunable filters, or acousto-optic tunable filters. They are minimally sensitive to polarization and their spectral isolation can reach 106. Here we thus present an innovative tool to collect SWIR hyperspectral data with high spectral and spatial resolution. This new instrument is based on a 3nm bandwidth Bragg Tunable Filter, continuously tunable from 1.0um and 2.5um. Because high spectral resolution also means less light per channel, a low noise custom HgCdTe (MCT) camera was also developed to meet the requirement of the filter. The high speed capability of more than 300 fps and the low operating temperature of 200K (deep cooled option to 77K) allow full frame 500 spectral channel datacube acquisitions in minimal time. Basic principle of this imaging filter will be reviewed as well as the custom MCT camera performances. High resolution hyperspectral measurements will be demonstrated between 1.0um and 2.5um on different objects.

  2. Effects of Moulding Sand Permeability and Pouring Temperatures on Properties of Cast 6061 Aluminium Alloy

    Directory of Open Access Journals (Sweden)

    Olawale Olarewaju Ajibola

    2015-01-01

    Full Text Available Effects of moulding sand permeabilities prepared from the combinations of four proportions of coarse and fine particle size mixtures and pouring temperatures varied from 700, 750, and 800 (±10°C were studied on the hardness, porosity, strength, and microstructure of cast aluminium pistons used in hydraulic brake master cylinder. Three sand moulds were prepared from each of the 80 : 20, 60 : 40, 40 : 60, and 20 : 80 ratios. The surfaces and microstructures of cast samples were examined using high resolution microscopic camera, metallurgical microscope with digital camera, and scanning electron microscope with EDX facilities. The best of the metallurgical properties were obtained from the combination of 80 : 20 coarse-fine sand ratio and 750 ± 10°C pouring temperature using as MgFeSi inoculant. An 8 : 25 ratio of coarse to fine grained eutectic aluminium alloy was obtained with enhanced metallographic properties. The cast alloy poured at 750 ± 1°C has a large number of fine grain formations assuming broom-resembling structures as shown in the 100 µm size SEM image.

  3. Potential applications of oral brush cytology with liquid-based technology: results from a cohort of normal oral mucosa.

    Science.gov (United States)

    Kujan, Omar; Desai, Mina; Sargent, Alexandra; Bailey, Andrew; Turner, Andrew; Sloan, Philip

    2006-09-01

    Fifty healthy volunteers were studied to assess the potential applications of oral brush sampling using liquid-based cytology. Three specimens from the buccal mucosa and lateral border of tongue were collected from each subject by using cervical brushes and brooms. The brush was immersed in a preservative fluid. The sample in the preservative fluid was processed according to the manufacturer's directions (SurePath, UK). Slides were stained by the Papanicolaou method and assessed for squamous cell adequacy by the same criteria used for cervical cytology screening. Immunocytochemical staining for FHIT (Fragile Histidine Triad) was applied in liquid-based preparations following the streptavidin-biotin-peroxidase method. Human papillomavirus (HPV) detection was performed using the Hybrid Capture 2 assay (Digene) and the PCR-based Roche AMPLICOR HPV Test. LBC preparation slides showed good sample preservation, specimen adequacy and visualization of cell morphology. Interestingly, nine cases showed borderline cytological abnormalities from apparently normal oral mucosa. All cases showed good quality positive FHIT immunoreactivity staining. All studied cases were high-risk HPV negative using HC2 assay method. However, the AMPLICOR Roche Test detected four samples with positive results for high-risk HPVs. Liquid-based cytology has potential as a screening tool for oral cancer and precancer. The method may also have applications for research and practice in the field of oral cancer and precancer. However a special custom-designed oral cytobrush is required.

  4. HIGH RESOLUTION AIRBORNE LASER SCANNING AND HYPERSPECTRAL IMAGING WITH A SMALL UAV PLATFORM

    Directory of Open Access Journals (Sweden)

    M. Gallay

    2016-06-01

    Full Text Available The capabilities of unmanned airborne systems (UAS have become diverse with the recent development of lightweight remote sensing instruments. In this paper, we demonstrate our custom integration of the state-of-the-art technologies within an unmanned aerial platform capable of high-resolution and high-accuracy laser scanning, hyperspectral imaging, and photographic imaging. The technological solution comprises the latest development of a completely autonomous, unmanned helicopter by Aeroscout, the Scout B1-100 UAV helicopter. The helicopter is powered by a gasoline two-stroke engine and it allows for integrating 18 kg of a customized payload unit. The whole system is modular providing flexibility of payload options, which comprises the main advantage of the UAS. The UAS integrates two kinds of payloads which can be altered. Both payloads integrate a GPS/IMU with a dual GPS antenna configuration provided by OXTS for accurate navigation and position measurements during the data acquisition. The first payload comprises a VUX-1 laser scanner by RIEGL and a Sony A6000 E-Mount photo camera. The second payload for hyperspectral scanning integrates a push-broom imager AISA KESTREL 10 by SPECIM. The UAS was designed for research of various aspects of landscape dynamics (landslides, erosion, flooding, or phenology in high spectral and spatial resolution.

  5. TRAUMATIC HIP DISLOCATION IN CHILDREN YOUNGER THAN 3 YEARS : A SERIES OF 3 CASES

    Directory of Open Access Journals (Sweden)

    Navin

    2015-03-01

    Full Text Available The musculoskeletal anatomy of children is qui te different from that of adults. The capsule, periosteum and other soft tissues surrounding the joints is very pliable and dislocations can occur even following trivial injuries . However dislocation occurring in a child population is a rare entity. There are a few case reports of hip dislocations in children < 3 years. We present here a series of 3 cases of paediatric hip dislocations which presented to us for management. The age of the children were between 24 - 27 months. Incidentally all 3 patients were b oys and all 3 were right sided dislocations. None of them had any associated fractures. Two of them had a fall from height and one child fell off a slide. All 3 patients presented within 5 hours following injury. Closed reduction under anaesthesia was perf ormed and post reduction the children were immobilized in a broom - stick plaster for 6 weeks. All children were followed up with serial x - rays and a MRI done immediately following reduction then at 6 and 18 months. There was no evidence of chondrolysis or a vascular necrosis in any of the children at the last follow - up. They all had full range of movements with no limitation of function. We conclude that with early recognition and early reduction of the hip within the golden period (6 hours the occurrence of complications can be prevented.

  6. ETHNOBOTANY OF THARUS OF DUDHWA NATIONAL PARK, INDIA

    Directory of Open Access Journals (Sweden)

    Bharati K Avinash

    2013-01-01

    Full Text Available Tharus are inhabited on southern foothills of the Himalayas along Indo-Nepal border. They have been using many plant species to meet their day-to-day needs. The aim of this study was to collect information on the traditional uses of different plants and to document the potential economic use of these plants. Fieldwork was conducted over a period of two years in Dudhwa National Park, utilizing the “transect walk” method of Participatory Rural Appraisal (PRA. The data was analyzed using the following techniques: frequency of citation and informant consensus factor (Fic. The present communication gives information on 86 species belonging to 38 families of plants used by Tharu tribes of Dudhwa National Park, Uttar Pradesh. The frequency of citation was very high for Alstonia scholaris (fire-wood, Antidesma acidum, Artocarpus lakoocha (edible, Bauhinia vahlii, Butea monosperma (food plate, Dendrocalamus strictus (hut preparation, Hibiscus cannabinus (rope, Oryza rufipogon (food, Phoenix acaulis (edible and Tamarix dioica (broom.

  7. Avaliação genética de indivíduos e progênies de cupuaçuzeiro no estado do Pará e estimativas de parâmetros genéticos Genetic evaluation of individuals and progenies of Theobroma grandiflorum in the state of Pará and estimates of genetic parameters

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2008-09-01

    seleção recorrente para melhorar, gradativamente, o nível de resistência. Parece suficiente considerar na seleção apenas o número de vassouras, não sendo necessário considerar o peso. A correlação entre resistência no fruto e na inflorescência foi alta (0.84, indicando algum controle genético comum aos dois caracteres. Foram identificadas progênies superiores, simultaneamente, para produção de frutos e resistência à vassoura.This paper deals with the genotypic evaluation of Theobroma grandiflorum progenies in the Pará State for the characters number of fruits (NF in four harvests, intensity of occurrence of witch's broom disease in the inflorescences (WBI and in the fruits (WBF and weight of branches with witch's broom (WWB. Also, it presents estimates of genetic parameters that allow to infer on the genetic control and level of genetic variability in the appraised germplasm. All the characters presented considerable genetic variability, with coefficients of genetic variation varying from 27% to 88% at progenies level and from 38% to 123% at individual level. This reveals excellent possibilities for the selection in that hybrid experimental population. The estimates of individual narrow sense heritabilities, in a harvest, varied from 25% to 54% and the individual repeatabilities for NF was equal at 35%. With the four accomplished harvests the individual heritability increased to 48%, providing selective accuracy of 70%, for the individuals' selection. The gain in efficiency, when using more than five crops is practically worthless. For NF, genetic gains above 60% can be obtained with the selection of the best five individuals. Individuals with annual production of 17 fruits can be selected, value which is much more superior to the general average of 10 fruits, found in the commercial plantations. Superior genetic gains can be obtained with clone propagation of the best individuals in relation to the sexual propagation and for the best individual the

  8. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    Because of its geological structure, in the Emilia-Romagna Region over 32,000 landslides have been identified. Several works have been made in order to control mass movement's dynamics and to secure of Reno and Lamone Mountain Basin Rivers, the road network and near by villages and towns. Most of the control works dealt with bioengineering practices: palisades piles, geotextiles, seedings, surface flow control works, dikes within main drainage ditches. In order to check about critical aspects related to the use of these techniques in the Apennines, a survey in this basins was designed with specific interest in the several kinds of works realised, in which plant species were mostly used and in the factors that affected the success or failure of the works. Territory encompasses steep slopes covered with woods to low reliefs covered with grasslands. It is characterized by prevailing clays, inducing instability, and arenaceous lithology with impermeable soils; drainage density is quite high and hillsides suffer extensive and severe erosion and slope stability problems. Chestnut woods mainly represent land use at higher altitudes, while coppice, pastures and crops are present on milder hillsides. The remaining part of the basin is covered by vineyards, orchards, ponds and urban areas, which are basically located in the valley floor. Precipitation events mainly consist of rainfall ranging between 950-1015 mm per year; few snowfalls occur during winter and a long dry season lasts from June until September. We have analyzed 187 works designed mainly for the consolidation of slope instabilities through a widespread enhancement of the vegetation cover. The surveyed works are classified as a function of their building features: it can be seen that cribwalls and palisades are by far the most common types, being the 24% and the 34% respectively of the works. As far as the most adopted plant species, they were silver willow (Salix alba), Spanish Broom (Spartium Junceum) and

  9. Mixobiota do Parque Nacional Serra de Itabaiana, SE, Brasil: Physarales Myxobiota of Serra de Itabaiana National Park, Sergipe State, Brazil: Physarales

    Directory of Open Access Journals (Sweden)

    Maria de Fátima de Andrade Bezerra

    2008-12-01

    Full Text Available Visando contribuir com informações sobre a diversidade de Myxomycetes em florestas úmidas e savanas Neotropicais, foram inventariadas as espécies de Physarales associadas a diferentes microhabitats no Parque Nacional Serra de Itabaiana, Sergipe, Brasil (10º40'52"S e 37º25'15"W, 180-670 m, 7966 ha. Durante 20 meses consecutivos foram efetuadas coletas de esporocarpos e de amostras de vários tipos de substratos a serem utilizados na preparação de 590 câmaras-úmidas. Coleções representativas do material estudado foram depositadas no Herbário UFP, com duplicatas no herbário MA - Fungi. A abundância e a constância de cada espécie foram determinadas. Apresenta-se uma lista comentada das sete espécies de Didymiaceae e 24 espécies de Physaraceae identificadas e sua distribuição no Brasil. As Physarales estão presentes em todos os microhabitats analisados, esporulando na estiagem e na estação chuvosa; fimícolas e suculentícolas foram registradas apenas em câmara-úmida. As espécies mais abundantes foram Physarum viride (Bull. Pers., P. stellatum (Massee G.W. Martin e P. cinereum (Batsch Pers. Exceto Diachea silvaepluvialis M.L. Farr, P. cinereume Physarum roseumBerk. & Broome, todos os táxons constituem primeira referência para Sergipe. Diderma rugosum (Rex T. Macbr., Didymium dubium Rostaf. e Physarum pulcherrimum Berk. & Ravenel estão sendo referidas pela primeira vez para o Brasil.The aims of this study were to obtain data on Myxomycete diversity in Neotropical forests and savannas and to determine the composition of the Physarales species assemblage associated with different microhabitats in Serra de Itabaiana National Park, Sergipe (10º 40'52"S; 37º 25'15"W, 180-670m alt., 7966 ha. During 20 months, sporocarps were collected and samples of substrata obtained for use in 590 moist-chamber cultures. Collections are deposited in the Herbaria UFP and MA - Fungi. The abundance and constancy of each species are given

  10. Slav beliefs on changelings

    Directory of Open Access Journals (Sweden)

    Radenković Ljubinko R.

    2002-01-01

    Full Text Available Beliefs and legends that certain mythological creatures - fairies, witches, the devil, (vile, veštice, đavo, boginka, mamuna, baenik, domovoj, leshi etc. can take away the child from the mother and exchange it for its own in the image of the abducted child, are widespread with the West and East Slavs, while with the South Slavs they are found only in the northern parts, in Pannonia. Such demonic child is most often called: podmeče (with the Serbs, podvršće (with the Croats, podmenek (with the Slovenians, odmienjec (with the Poles, odminok (with the Ukrainians, obmen (with the Russians, etc. According to the folk beliefs, a changeling differs from the other children by its sluggish growth, voraciousness, and persistent desire to harm or spite other members of the household. Slav legends mention the ways of stealing the human and planting the demonic child (a, recognizing the demonic child (b, and disposing of it and restoring the rightful child (c. In order to prevent the demon from exchanging her child, the mother must observe certain rules of conduct during pregnancy and in the 40 days following the childbirth. Certain measures of magical protection are also undertaken, as: placing sharp iron objects near the nursing woman, then brooms, leaving the candle to burn all night, burning frankincense in her presence, sprinkling her with holy water, etc. The legends on changelings were most probably adopted by the Slavs from the neighboring western peoples (Germans, and included in the already present beliefs that the birth of a child is a gift from the other world, and that the mother must take great care of the gift and be grateful for it. Otherwise, the one bestowing the gift may take it away as well.

  11. Breeding and Application of a New Yam Cultivar “Minghuai No.1”%淮山药新品种“明淮1号”的选育及应用

    Institute of Scientific and Technical Information of China (English)

    陈芝华; 华树妹; 李丽红; 雷伏贵; 贺佩珍; 曹奕鸯; 莫智龙

    2016-01-01

    “明淮1号”是从福建当地品种上杭扫把薯的自然变异群体中通过单株选择和无性繁殖培育而成的粮菜兼用型山药新品种。该品种属于晚熟淮山药品种,为紫肉扁块状参薯,在连续两年的区域试验和生产试验中比对照“上杭扫把薯”增产显著,具有产量高、耐贫瘠、抗逆性强、适应性广和适合机械化栽培等特点,具有较高的开发应用前景。%“Minghuai No.1” was a new cultivar with double dual-purpose of staple food and vegetable yam, through which selection individual plant and asexual reproduction from a group of natural variation population of local broom yam in Shanghang city, Fujian province. The cultivar is a late maturity yam variety with purple lfeshed and lfat block, which belong to the speices ofDioscorea alata L.. In the two years of regional and production trials, the new cultivar had higher yield, poorer tolerance, stronger resistance, wider adaptability and better suitable for mechanical cultivation than the control. According to these, the new cultivar “Minghuai No.1” had a high development and application prospects.

  12. Mid and thermal infrared remote sensing at the Jet Propulsion Laboratory

    Science.gov (United States)

    Johnson, William R.; Hook, Simon J.

    2016-05-01

    The mid and thermal infrared (MTIR) for the Earth surface is defined between 3 and 14µm. In the outer solar system, objects are colder and their Planck response shifts towards longer wavelengths. Hence for these objects (e.g. icy moons, polar caps, comets, Europa), the thermal IR definition usually stretches out to 50µm and beyond. Spectroscopy has been a key part of this scientific exploration because of its ability to remotely determine elemental and mineralogical composition. Many key gas species such as methane, ammonia, sulfur, etc. also have vibrational bands which show up in the thermal infrared spectrum above the background response. Over the past few decades, the Jet Propulsion Laboratory has been building up a portfolio of technology to capture the MTIR for various scientific applications. Three recent sensors are briefly reviewed: The airborne Hyperspectral thermal emission spectrometer (HyTES), the ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station (ECOSTRESS) and Mars Climate Sounder (MCS)/DIVINER. Each of these sensors utilize a different technology to provide a remote sensing product based on MTIR science. For example, HyTES is a push-brooming hyperspectral imager which utilizes a large format quantum well infrared photodetector (QWIP). The goal is to transition this to a new complementary barrier infrared photodetector (CBIRD) with a similar long wave cut-off and increased sensitivity. ECOSTRESS is a push-whisk Mercury Cadmium Telluride (MCT) based high speed, multi-band, imager which will eventually observe and characterize plant/vegetation functionality and stress index from the International Space Station (ISS) across the contiguous United States (CONUS). MCS/DIVINER utilizes thermopile technology to capture the thermal emission from the polar caps and shadow regions of the moon. Each sensor utilizes specific JPL technology to capture unique science.

  13. Structural Control of Sand Bodies and Deep Exploration for Oil and Gas

    Institute of Scientific and Technical Information of China (English)

    Cheng Rihui; Lin Changsong; Zheng Herong

    2002-01-01

    The structural styles can be used to analyses and predict developments and distributions of sand bodies in a rift basin. The dynamic process of faulting and sedimentation can be expressed as follow: the basin topography controlled by fault activity can control water dynamics; which in turn affect the transport and sedimentation of sediments. The corresponding analysis between structural styles and sand depositional types includes the following aspects: (1) in section, the corresponding between development of fault terraces and sand depositional types; (2) in plane, the relationship between faults' association and distributions of sand bodies. There are four types of terrace styles to be identified. They are Steep Slope Single Fault Terrace (SSSFT), Steep Slope Multiple Fault Terrace (SSMFT), Gentle Slope (GS) and Gentle Slope Multiple Fault Terrace (GSMFT), which also can be divided into six subtypes by the timing of the faults activities and the directions of their activity migrations (basinward and landward or marginward). They correspond to the following sand depositions such as alluvial fan, fan delta and turbidite fan etc.. The analysis of structure-sedimentation is a discussion on the rank Ⅲ sequence evolution under the condition of pulsing or episodic fault activities. It has been recognized four plane fault associations such as the comb, the broom, the fork and the fault-fold association as well as the corresponding sand distributions. Structural-sedimentary models above mentioned are significant for the deep oil and gas exploration when lacking of the drill data. It may reduce multiple resolutions in the interpretation of seismic-sedimentary facies and promote sand predictions through the constraints of the structural styles of the basin units. The structural-sedimentary pattern can be used as a geological model in oil and gas exploration in the rift basins.

  14. ETHNOBOTANICAL USES OF LANTANA TRIFOLIA L. AND SIDA CUNEIFOLIA ROXB. IN MUKUNGWE AND WABINYONYI SUB-COUNTIES OF CENTRAL UGANDA

    Directory of Open Access Journals (Sweden)

    Rebecca Nalubega

    2013-06-01

    Full Text Available Aim: This was an ethnobotanical study that was carried out to establish the traditional uses of Lantana trifolia L. and Sida cuneifolia Roxb. plants in selected parts of Central Uganda. Methods: The ethnobotanical study was done in August and September, 2012 in Mukungwe and Wabinyonyi sub-counties in Masaka and Nakasongola Districts respectively located in Central Uganda. Study sites and respondents were purposefully selected and information was obtained through semi-structured interview guides, key informant interview guides as well as observations. Eighty respondents were considered for semi-structured interviews and 15 for key informant interviews. Results: Seven ethnobotanical uses for Lantana trifolia were cited by respondents and majority (46.25% of them used it as a herbal remedy. As a herbal remedy, Lantana trifolia managed 13 human disease conditions and mainly used in the management of cough and common colds by 22.5% of the respondents. Four ethnobotanical uses were cited for Sida cuneifolia and majority of the respondents (62.5% used it as a herbal remedy as well as sweeping brooms. As a herbal remedy, Sida cuneifolia was reported to be useful in management of 12 disease conditions, fractures and sprains (bone setting being mentioned by the majority of the of respondents (36.25 %. Conclusion: In conclusion, Lantana trifolia and Sida cuneifolia were culturally important ethnomedicines. Scientific validation of traditional claims as well as conservation of these plants should be encouraged in order to preserve and promote their use. [J Intercult Ethnopharmacol 2013; 2(3.000: 155-164

  15. Lipid class and fatty acid content of the leptocephalus larva of tropical eels.

    Science.gov (United States)

    Deibel, D; Parrish, C C; Grønkjær, P; Munk, P; Nielsen, T Gissel

    2012-06-01

    The leptocephalus larva of eels distinguishes the elopomorph fishes from all other bony fishes. The leptocephalus is long lived and increases in size primarily through the synthesis and deposition of glycosaminoglycans. Energy stored during the larval stage, in the form of glycosaminoglycan and lipids, is required to fuel migration, metamorphosis and metabolism of the subsequent glass eel stage. Despite the importance of energy storage by leptocephali for survival and recruitment, their diet, condition and lipid content and composition is essentially unknown. To gain further insight into energy storage and condition of leptocephali, we determined the lipid class and fatty acid concentration of larvae collected on a cross-shelf transect off Broome, northwestern Australia. The total lipid concentration of two families and four sub-families of leptocephali ranged from 2.7 to 7.0 mg g wet weight(-1), at the low end of the few published values. Phospholipid and triacylglycerol made up ca. 63 % of the total lipid pool. The triacylglycerol:sterol ratio, an index of nutritional condition, ranged from 0.9 to 3.7, indicating that the leptocephali were in good condition. The predominant fatty acids were 16:0 (23 mol%), 22:6n-3 (docosahexaenoic acid, DHA, 16 mol%), 18:0 (8.2 mol%), 20:5n-3 (eicosapentaenoic acid, EPA, 6.7 mol%), 18:1n-9 (6.4 mol%) and 16:1n-7 (6.3 mol%). The DHA:EPA ratio ranged from 2.4 to 2.9, sufficient for normal growth and development of fish larvae generally. The leptocephali had proportions of bacterial markers >4.4 %, consistent with the possibility that they consume appendicularian houses or other marine snow that is bacteria rich.

  16. HPLC-DAD-MS analysis of dyes identified in textiles from Mount Athos.

    Science.gov (United States)

    Mantzouris, Dimitrios; Karapanagiotis, Ioannis; Valianou, Lemonia; Panayiotou, Costas

    2011-03-01

    Organic colorants contained in 30 textiles (16th to early 20th century) from the monastery of Simonos Petra (Mount Athos) have been investigated using high-performance liquid chromatography equipped with diode-array detection and mass spectrometry (HPLC-DAD-MS). The components of natural dyes identified in samples treated by the standard HCl dyestuff extraction method were: alizarin, apigenin, butein, carminic acid, chrysoeriol, dcII, dcIV, dcVII, ellagic acid, emodin, fisetin, flavokermesic acid, fustin, genistein, haematein derivative (Hae'), indigotin, indirubin, isoliquiritigenin, isorhamnetin, kaempferide, kaempferol, kermesic acid, luteolin, naringenin, purpurin, quercetin, rhamnazin, rhamnetin, sulfuretin, and type B and type C compounds (last two are markers for Caesalpinia trees). Early, semi-synthetic dyes, for example indigo carmine, fuchsin components, and rhodamine B were identified in objects dated late 19th to early 20th century. A dyestuff extraction method which involves use of TFA, instead of HCl, was applied to selected historical samples, showing that the mild method enables efficient extraction of weld (Reseda luteola L.) and dyer's broom (Genista tinctoria L.) glycosides. The marker compound (Hae') for logwood (Haematoxylum campechianum L.) identification after treatment with HCl was investigated by liquid chromatography coupled to mass spectrometry (LC-MS) in negative electrospray ionization (LC-MS-ESI(-)) mode. LC-MS in negative atmospheric pressure chemical ionization (LC-MS-APCI(-)) mode was used, probably for the first time, to investigate cochineal (Dactylopius coccus Costa) samples. Positive electrospray ionization (LC-MS-ESI(+)) mode was used for identification of fuchsin components. Detailed HPLC-DAD studies were performed on young fustic (Cotinus coggygria Scop.) and Persian berries (Rhamnus trees).

  17. Unexpectedly Diverse Mesorhizobium Strains and Rhizobium leguminosarum Nodulate Native Legume Genera of New Zealand, while Introduced Legume Weeds Are Nodulated by Bradyrhizobium Species

    Science.gov (United States)

    Weir, Bevan S.; Turner, Susan J.; Silvester, Warwick B.; Park, Duck-Chul; Young, John M.

    2004-01-01

    The New Zealand native legume flora are represented by four genera, Sophora, Carmichaelia, Clianthus, and Montigena. The adventive flora of New Zealand contains several legume species introduced in the 19th century and now established as serious invasive weeds. Until now, nothing has been reported on the identification of the associated rhizobia of native or introduced legumes in New Zealand. The success of the introduced species may be due, at least in part, to the nature of their rhizobial symbioses. This study set out to address this issue by identifying rhizobial strains isolated from species of the four native legume genera and from the introduced weeds: Acacia spp. (wattles), Cytisus scoparius (broom), and Ulex europaeus (gorse). The identities of the isolates and their relationship to known rhizobia were established by comparative analysis of 16S ribosomal DNA, atpD, glnII, and recA gene sequences. Maximum-likelihood analysis of the resultant data partitioned the bacteria into three genera. Most isolates from native legumes aligned with the genus Mesorhizobium, either as members of named species or as putative novel species. The widespread distribution of strains from individual native legume genera across Mesorhizobium spp. contrasts with previous reports implying that bacterial species are specific to limited numbers of legume genera. In addition, four isolates were identified as Rhizobium leguminosarum. In contrast, all sequences from isolates from introduced weeds aligned with Bradyrhizobium species but formed clusters distinct from existing named species. These results show that native legume genera and these introduced legume genera do not have the same rhizobial populations. PMID:15466541

  18. Specific PCR and real-time PCR assays for detection and quantitation of 'Candidatus Phytoplasma phoenicium'.

    Science.gov (United States)

    Jawhari, Maan; Abrahamian, Peter; Sater, Ali Abdel; Sobh, Hana; Tawidian, Patil; Abou-Jawdah, Yusuf

    2015-02-01

    Almond witches' broom (AlmWB) is a fast-spreading lethal disease of almond, peach and nectarine associated with 'Candidatus Phytoplasma phoenicium'. The development of PCR and quantitative real-time PCR (qPCR) assays for the sensitive and specific detection of the phytoplasma is of prime importance for early detection of 'Ca. P. phoenicium' and for epidemiological studies. The developed qPCR assay herein uses a TaqMan(®) probe labeled with Black Hole Quencher Plus. The specificity of the PCR and that of the qPCR detection protocols were tested on 17 phytoplasma isolates belonging to 11 phytoplasma 16S rRNA groups, on samples of almond, peach, nectarine, native plants and insects infected or uninfected with the phytoplasma. The developed assays showed high specificity against 'Ca. P. phoenicium' and no cross-reactivity against any other phytoplasma, plant or insect tested. The sensitivity of the developed PCR and qPCR assays was similar to the conventional nested PCR protocol using universal primers. The qPCR assay was further validated by quantitating AlmWB phytoplasma in different hosts, plant parts and potential insect vectors. The highest titers of 'Ca. P. phoenicium' were detected in the phloem tissues of stems and roots of almond and nectarine trees, where they averaged from 10(5) to 10(6) genomic units per nanogram of host DNA (GU/ng of DNA). The newly developed PCR and qPCR protocols are reliable, specific and sensitive methods that are easily applicable to high-throughput diagnosis of AlmWB in plants and insects and can be used for surveys of potential vectors and alternative hosts.

  19. 云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析

    Institute of Scientific and Technical Information of China (English)

    和志娇; 蔡红; 陈海如; 和根强; 吴华英; 唐丽明

    2005-01-01

    应用植原体核糖体蛋白基因通用引物对rpF1/rpR1,对采自云南省曲靖市的泡桐丛枝病植原体DNA(PaWB-QJ)进行PCR扩增,得到1.3 kb的特异片段,证明此病株中存在植原体。将此片段与pGEM-T Easy载体连接并转化大肠杆菌 JM109感受态细胞,进行PCR鉴定、核糖体蛋白基因部分核苷酸序列测定及分析。结果表明,该株系(PaWB-QJ)核糖体蛋白基因片段长1 244 bp,包含rps19、rp122和rps3基因。对PaWB-QJ株系的核糖体蛋白基因序列的同源性比较结果显示与 16S rI-B亚组的翠菊黄化(Aster yellows,AY)、长春花黄化(Periwinkle yellows,PY)和泡桐丛枝德国株系(Paulownia witches’-broom,PaWB-German)的亲缘关系最近,达到99.0%以上,而与其它组中的株系明显低于97.0%,所以认为该植原体株系属于翠菊黄化组B亚组(16SrI-B)。

  20. Programmable matched filter and Hadamard transform hyperspectral imagers based on micro-mirror arrays

    Energy Technology Data Exchange (ETDEWEB)

    Love, Steven P [Los Alamos National Laboratory

    2008-01-01

    Hyperspectral imaging (HSI), in which each pixel contains a high-resolution spectrum, is a powerful technique that can remotely detect, identify, and quantify a multitude of materials and chemicals. The advent of addressable micro-mirror arrays (MMAs) makes possible a new class of programmable hyperspectral imagers that can perform key spectral processing functions directly in the optical hardware, thus alleviating some of HSI's high computational overhead, as well as offering improved signal-to-noise in certain important regimes (e.g. when using uncooled infrared detectors). We have built and demonstrated a prototype UV-Visible micro-mirror hyperspectral imager that is capable not only of matched-filter imaging, but also of full hyperspectral imagery via the Hadamard transform technique. With this instrument, one can upload a chemical-specific spectral matched filter directly to the MMA, producing an image showing the location of that chemical without further processing. Target chemicals are changeable nearly instantaneously simply by uploading new matched-filter patterns to the MMA. Alternatively, the MMA can implement Hadamard mask functions, yielding a full-spectrum hyperspectral image upon inverting the transform. In either case, the instrument can produce the 2D spatial image either by an internal scan, using the MMA itself, or with a traditional external push-broom scan. The various modes of operation are selectable simply by varying the software driving the MMA. Here the design and performance of the prototype is discussed, along with experimental results confirming the signal-to-noise improvement produced by the Hadamard technique in the noisy-detector regime.

  1. Airborne measurements in the longwave infrared using an imaging hyperspectral sensor

    Science.gov (United States)

    Allard, Jean-Pierre; Chamberland, Martin; Farley, Vincent; Marcotte, Frédérick; Rolland, Matthias; Vallières, Alexandre; Villemaire, André

    2008-08-01

    Emerging applications in Defense and Security require sensors with state-of-the-art sensitivity and capabilities. Among these sensors, the imaging spectrometer is an instrument yielding a large amount of rich information about the measured scene. Standoff detection, identification and quantification of chemicals in the gaseous state is one important application. Analysis of the surface emissivity as a means to classify ground properties and usage is another one. Imaging spectrometers have unmatched capabilities to meet the requirements of these applications. Telops has developed the FIRST, a LWIR hyperspectral imager. The FIRST is based on the Fourier Transform technology yielding high spectral resolution and enabling high accuracy radiometric calibration. The FIRST, a man portable sensor, provides datacubes of up to 320x256 pixels at 0.35mrad spatial resolution over the 8-12 μm spectral range at spectral resolutions of up to 0.25cm-1. The FIRST has been used in several field campaigns, including the demonstration of standoff chemical agent detection [http://dx.doi.org/10.1117/12.795119.1]. More recently, an airborne system integrating the FIRST has been developed to provide airborne hyperspectral measurement capabilities. The airborne system and its capabilities are presented in this paper. The FIRST sensor modularity enables operation in various configurations such as tripod-mounted and airborne. In the airborne configuration, the FIRST can be operated in push-broom mode, or in staring mode with image motion compensation. This paper focuses on the airborne operation of the FIRST sensor.

  2. The NASA enhanced MODIS airborne simulator

    Science.gov (United States)

    Ellis, Thomas A.; Myers, Jeffrey; Grant, Patrick; Platnick, Steven; Guerin, Daniel C.; Fisher, John; Song, Kai; Kimchi, Joseph; Kilmer, Louis; LaPorte, Daniel D.; Moeller, Christopher C.

    2011-10-01

    The new NASA Enhanced MODIS Airborne Simulator (eMAS) is based on the legacy MAS system, which has been used extensively in support of the NASA Earth Observing System program since 1995. eMAS consists of two separate instruments designed to fly together on the NASA ER-2 and Global Hawk high altitude aircraft. The eMAS-IR instrument is an upgraded version of the legacy MAS line-scanning spectrometer, with 38 spectral bands in the wavelength range from 0.47 to 14.1 μm. The original LN2-cooled MAS MWIR and LWIR spectrometers are replaced with a single vacuum-sealed, Stirling-cooled assembly, having a single MWIR and twelve LWIR bands. This spectrometer module contains a cold optical bench where both dispersive optics and detector arrays are maintained at cryogenic temperatures to reduce infrared background noise, and ensure spectral stability during high altitude airborne operations. The EMAS-HS instrument is a stand-alone push-broom imaging spectrometer, with 202 contiguous spectral bands in the wavelength range from 0.38 to 2.40 μm. It consists of two Offner spectrometers, mated to a 4-mirror anastigmatic telescope. The system has a single slit, and uses a dichroic beam-splitter to divide the incoming energy between VNIR and SWIR focal plane arrays. It will be synchronized and bore-sighted with the IR line-scanner, and includes an active source for monitoring calibration stability. eMAS is intended to support future satellite missions including the Hyperspectral Infrared Imager ( HyspIRI,) the National Polar-orbiting Operational Environmental Satellite System (NPOESS) Preparatory Project (NPP,) and the follow-on Joint Polar Satellite System (JPSS.)

  3. Extreme drought changes in Southwest China from 1960 to 2009%1960—2009年西南地区极端干旱气候变化

    Institute of Scientific and Technical Information of China (English)

    ZHANG Mingjun; HE Jinyun; WANG Baolong; WANG Shengjie; LI Shanshan; LIU Wenli; MA Xuening

    2013-01-01

    @@%Based on the daily data of temperature and precipitation of 108 meteorological stations in Southwest China from 1960 to 2009,we calculate the monthly and yearly surface humid indexes,as well as the extreme drought frequency.According to the data,the temporal and spatial characteristics of the extreme drought frequency in inter-annual,inter-decadal,summer monsoon period and winter monsoon period are analyzed.The results are indicated as follows.(1) In general,the southwestern Sichuan Basin,southern Hengduan Mountains,southern coast of Guangxi and northern Guizhou are the areas where the extreme drought frequency has significantly increased in the past 50 years.As for the decadal change,from the 1960s to the 1980s the extreme drought frequency has presented a decreasing trend,while the 1990s is the wettest decade and the whole area is turning wet.In the 2000s,the extreme drought frequency rises quickly,but the regional differences reduce.(2) During summer monsoon period,the extreme drought frequency is growing,which generally occurs in the high mountains around the Sichuan Basin,most parts of Guangxi and "the broom-shaped mountains" in Yunnan.It is distinct that the altitude has impacts on the extreme drought frequency; during winter monsoon period,the area is relatively wet and the extreme drought frequency is decreasing.(3) During summer monsoon period,the abrupt change is observed in 2003,whereas the abrupt change during winter monsoon period is in 1989.The annual extreme drought frequency variation is a superposition of abrupt changes during summer monsoon and winter monsoon periods.The departure sequence vibration of annual extreme drought frequency is quasi-5 years and quasi-12 years.

  4. Evaluation of electron beam radiation effects in the incorporation of Piassava fibers (Attalea funifera Mart.) on ethylene vinyl alcohol copolymer (EVOH); Avaliacao dos efeitos da radiacao ionizante por feixe de eletrons na incorporacao de fibra de Piacava (Attalea funifera Mart.) no copolimero de etileno e alcool vinilico (EVOH)

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Beatriz Ribeiro

    2012-07-01

    The aim of this work is to explore the contribution of ionizing radiation in the incorporation of piassava fibers (derived from wastes from broom factories) in the ethylene vinyl alcohol copolymer (EVOH), to obtain a composite material, {sup E}VOH-Piassava{sup ,} for packaging applications. The interest in this fiber is due to its inherent properties and morphology as well as being biodegradable. The EVOH composite reinforced with 5% or 10%, by weight of piassava fiber powder with a particle size equal to or less than 125 {mu}m, untreated or treated with 1% silane, were obtained by extrusion, using a twin screw extruder. For carrying out the tensile tests and oxygen barrier of the materials obtained, films were produced from composite materials and EVOH resin was obtained using the process of flat extrusion. Samples of EVOH and the composites were irradiated using an electron accelerator, at doses from 0 to 500 kGy. The results of SEM analysis and X-ray diffraction of composites suggest a good fiber-matrix adhesion, which can be also confirmed by its higher tensile strength at break, compared to that of neat EVOH. The results showed that the incorporation of piassava fibers can promote better oxygen barrier compared to the neat EVOH, in conditions of high humidity. It was observed that, after irradiation, the EVOH film showed better oxygen barrier in high humidity condition, when compared to the non-irradiated EVOH under the same condition, indicating radiation as an effective treatment to improve oxygen barrier in the studied conditions. (author)

  5. First Microsatellite Markers Developed from Cupuassu ESTs: Application in Diversity Analysis and Cross-Species Transferability to Cacao.

    Directory of Open Access Journals (Sweden)

    Lucas Ferraz Dos Santos

    Full Text Available The cupuassu tree (Theobroma grandiflorum (Willd. ex Spreng. Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified. The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches' broom disease, as well as to pulp quality (SST/ATT values. Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively.

  6. First Microsatellite Markers Developed from Cupuassu ESTs: Application in Diversity Analysis and Cross-Species Transferability to Cacao.

    Science.gov (United States)

    Ferraz Dos Santos, Lucas; Moreira Fregapani, Roberta; Falcão, Loeni Ludke; Togawa, Roberto Coiti; Costa, Marcos Mota do Carmo; Lopes, Uilson Vanderlei; Peres Gramacho, Karina; Alves, Rafael Moyses; Micheli, Fabienne; Marcellino, Lucilia Helena

    2016-01-01

    The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches' broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively.

  7. Sentinel-2 diffuser on-ground calibration

    Science.gov (United States)

    Mazy, E.; Camus, F.; Chorvalli, V.; Domken, I.; Laborie, A.; Marcotte, S.; Stockman, Y.

    2013-10-01

    The Sentinel-2 multi-spectral instrument (MSI) will provide Earth imagery in the frame of the Global Monitoring for Environment and Security (GMES) initiative which is a joint undertaking of the European Commission and the Agency. MSI instrument, under Astrium SAS responsibility, is a push-broom spectro imager in 13 spectral channels in VNIR and SWIR. The instrument radiometric calibration is based on in-flight calibration with sunlight through a quasi Lambertian diffuser. The diffuser covers the full pupil and the full field of view of the instrument. The on-ground calibration of the diffuser BRDF is mandatory to fulfil the in-flight performances. The diffuser is a 779 x 278 mm2 rectangular flat area in Zenith-A material. It is mounted on a motorised door in front of the instrument optical system entrance. The diffuser manufacturing and calibration is under the Centre Spatial of Liege (CSL) responsibility. The CSL has designed and built a completely remote controlled BRDF test bench able to handle large diffusers in their mount. As the diffuser is calibrated directly in its mount with respect to a reference cube, the error budget is significantly improved. The BRDF calibration is performed directly in MSI instrument spectral bands by using dedicated band-pass filters (VNIR and SWIR up to 2200 nm). Absolute accuracy is better than 0.5% in VNIR spectral bands and 1% in SWIR spectral bands. Performances were cross checked with other laboratories. The first MSI diffuser for flight model was calibrated mid 2013 on CSL BRDF measurement bench. The calibration of the diffuser consists mainly in thermal vacuum cycles, BRDF uniformity characterisation and BRDF angular characterisation. The total amount of measurement for the first flight model diffuser corresponds to more than 17500 BRDF acquisitions. Performance results are discussed in comparison with requirements.

  8. 3MI OPD optical design: concept and performances

    Science.gov (United States)

    Gabrieli, Riccardo; Bartoli, Alessandro; Maiorano, Michele; Bruno, Umberto; Belli, Fabio; Bove, Giuseppe; Caruso, Alberto; Calamai, Luciano; Manolis, Ilias; Labate, Demetrio

    2015-09-01

    The Multi-Viewing, Multi-Channel, Multi-Polarisation Imager (3MI) is an imaging radiometer for the ESA/Eumetsat MetOp-SG programme. Based on the heritage of POLDER/PARASOL, 3MI will collect global observations of the top-of-atmosphere polarised bi-directional reflectance distribution function in 12 spectral bands, by observing the same target from multiple views using a push-broom scanning concept. In order to mitigate any technological risks associated with the 3MI instrument development, an Elegant Breadboard of representative form, function and performance to the 3MI VNIR lens was foreseen in the frame of the Optics Pre- Development (OPD) activity. The optical design and the performance results of the OPD VNIR lens are presented, from the top level requirements flow-down to the optical design solution and concept adopted. The large FOV and image irradiance uniformity, the extended VNIR spectral range, combined with the demanding polarisation and stray-light requirements are the main design drivers. The design concept is based on a Galilean telescope coupled to a focusing group. The aperture stop, placed in between, is located in such a way that the system is telecentric in image space. The system exhibits a fine control of the entrance pupil size as a function of the FOV, low distortion and correction of lateral chromatic aberration. Polarisation related performances are achieved by low polarisation sensitivity and low retardance anti-reflection coatings, as well as by a proper selection of glass material properties.

  9. Darwin’s legacy in South African evolutionary biology

    Directory of Open Access Journals (Sweden)

    S. D. Johnson

    2010-02-01

    Full Text Available In the two decades after publication of the Origin of Species, Charles Darwin facilitated the publication of numerous scientific papers by settler naturalists in South Africa. This helped to establish the strong tradition of natural history which has characterised evolutionary research in South African museums, herbaria and universities. Significant developments in the early 20th century included the hominid fossil discoveries of Raymond Dart, Robert Broom, and others, but there was otherwise very little South African involvement in the evolutionary synthesis of the 1930s and 1940s. Evolutionary biology developed into a distinct discipline in South Africa during the 1970s and 1980s when it was dominated by mammalian palaeontology and a vigorous debate around species concepts. In the post-apartheid era, the main focus of evolutionary biology has been the construction of phylogenies for African plants and animals using molecular data, and the use of these phylogenies to answer questions about taxonomic classification and trait evolution. South African biologists have also recently contributed important evidence for some of Darwin’s ideas about plant–animal coevolution, sexual selection, and the role of natural selection in speciation. A bibliographic analysis shows that South African authors produce 2–3% of the world’s publications in the field of evolutionary biology, which is much higher than the value of about 0.5% for publications in all sciences. With its extraordinary biodiversity and well-developed research infrastructure, South Africa is an ideal laboratory from which to advance evolutionary research.

  10. Recombinant β-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth.

    Science.gov (United States)

    Britto, Dahyana Santos; Pirovani, Carlos Priminho; Andrade, Bruno Silva; Dos Santos, Tassiara Pereira; Pungartnik, Cristina; Cascardo, Júlio Cezar M; Micheli, Fabienne; Gesteira, Abelmon S

    2013-09-01

    In this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a β-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of β-1,3-1,4-glucanases and presented high protein identity with β-1,3-1,4-glucanases from other plant species. Molecular modeling of TcGlu2 showed an active site of 13 amino acids typical of glucanase with β-1,3 and 1,4 action mode. The recombinant cDNA TcGlu2 obtained by heterologous expression in Escherichia coli and whose sequence was confirmed by mass spectrometry, has a molecular mass of about 22 kDa (with His-Tag) and showed antifungal activity against the fungus Moniliophthora perniciosa, causal agent of the witches' broom disease in cacao. The integrity of the hyphae membranes of M. perniciosa, incubated with protein TcGlu2, was analyzed with propidium iodide. After 1 h of incubation, a strong fluorescence emitted by the hyphae indicating the hydrolysis of the membrane by TcGlu2, was observed. To our knowledge, this is the first study of a cacao β-1,3-1,4-glucanase expression in heterologous system and the first analysis showing the antifungal activity of a β-1,3-1,4-glucanase, in particular against M. perniciosa.

  11. 'There's the record, closed and final': Rough for Theatre II as Psychiatric Encounter.

    Science.gov (United States)

    Heron, Jonathan; Broome, Matthew

    2016-06-01

    A co-authored collaboration between a theatre practitioner and a clinical psychiatrist, this paper will examine Rough for Theatre II (RFTII) and Beckett's demonstration of the way records are used to understand the human subject. Using Beckett's play to explore interdisciplinary issues of embodiment and diagnosis, the authors will present a dialogue that makes use of the 'best sources' in precisely the same manner as the play's protagonists. One of those sources will be Beckett himself, as Heron will locate the play in its theatrical context through reflections upon his own practice (with Fail Better Productions, UK) as well as recent studies such as Beckett, Technology and the Body (Maude 2009) and Performing Embodiment in Samuel Beckett's Drama (McMullan 2010); another source will be the philosopher Wilhelm Windleband, whose 1901 History of Philosophy was read and noted upon by Beckett in the 1930s, as Broome will introduce a philosophical and psychiatric context to the exchange. Windelband is now a neglected figure in philosophy; but as one of the key figures of Neo-Kantianism in the late 19(th) century, his work was an important impetus to that of Rickert, Weber and Heidegger. Specifically, Windelband gives us the distinction between idiographic and nomothetic understanding of individuals, an approach that is of relevance to the psychiatric encounter. This academic dialogue will consider tensions between subjectivity and objectivity in clinical and performance practice, while examining Beckett's analysis of the use of case notes and relating them back to Windelband's ideas on the understanding of others. The dialogue took place in 2011 at the University of Warwick, and has since been edited by the authors.

  12. The pharmacology of Malo maxima jellyfish venom extract in isolated cardiovascular tissues: A probable cause of the Irukandji syndrome in Western Australia.

    Science.gov (United States)

    Li, Ran; Wright, Christine E; Winkel, Kenneth D; Gershwin, Lisa-Ann; Angus, James A

    2011-03-25

    The in vitro cardiac and vascular pharmacology of Malo maxima, a newly described jellyfish suspected of causing Irukandji syndrome in the Broome region of Western Australia, was investigated in rat tissues. In left atria, M. maxima crude venom extract (CVE; 1-100μg/mL) caused concentration-dependent inotropic responses which were unaffected by atropine (1μM), but significantly attenuated by tetrodotoxin (TTX; 0.1μM), propranolol (1μM), Mg(2+) (6mM) or calcitonin gene-related peptide antagonist (CGRP(8-37); 1μM). CVE caused no change in right atrial rate until 100μg/mL, which elicited bradycardia. This was unaffected by atropine, TTX, propranolol or CGRP(8-37). In the presence of Mg(2+), CVE 30-100μg/mL caused tachycardia. In small mesenteric arteries CVE caused concentration-dependent contractions (pEC(50) 1.03±0.07μg/mL) that were unaffected by prazosin (0.3μM), ω-conotoxin GVIA (0.1μM) or Mg(2+) (6mM). There was a 2-fold increase in sensitivity in the presence of CGRP(8-37) (3μM). TTX (0.1μM), box jellyfish Chironex fleckeri antivenom (92.6U/mL) and benextramine (3μM) decreased sensitivity by 2.6, 1.9 and 2.1-fold, respectively. CVE-induced maximum contractions were attenuated by C. fleckeri antivenom (-22%) or benextramine (-49%). M. maxima CVE appears to activate the sympathetic, but not parasympathetic, nervous system and to stimulate sensory nerve CGRP release in left atria and resistance arteries. These effects are consistent with the catecholamine excess thought to cause Irukandji syndrome, with additional actions of CGRP release.

  13. Reproduction of an animal model of landmine blast injuries

    Directory of Open Access Journals (Sweden)

    Sen ZHANG

    2014-03-01

    Full Text Available Objective To reproduce an animal model of landmine blast injuries for studying its mechanism and characteristics. Methods Fifteen healthy New Zealand white rabbits (body weight 1.9-2.4 kg were prepared as experimental animals. Punctiform burster was used to simulate the landmine, and it was electrically detonated far away to produce landmine blast injuries on unilateral hind limb of rabbits in upright state. The vital signs before and 5min, 15min, 30min, 45min, 1h, 2h, 3h, 6h, 9h and 12h after injuries were recorded. Autopsy of dead animals was performed immediately and the survivors were sacrificed for pathological examination 6h and 12h after the injury. Macroscopic and microscopic changes in the injured limb and distant organs were observed. Fifteen random adult body weights were generated by random number table, and the explosive energy of M14 landmine (about 29g TNT explosive energy was simulated, to compare the ratio of explosive force equivalent to weight calculated between experimental animals and randomly selected adults. Results No significant change in blood pressure was observed at different time points before and after injuries. A broom-like change was found in the injured limb by the general observation. The subareas and pathological changes of injured limb coincided with the typical limb injuries produced by landmine explosion. Damage in different degrees was found in distant organs, and the wound characteristics and injury of major organs were in accordance with the reports of relevant literature. The ratio of explosive equivalent to weight of experimental animals (0.50±0.04g TNT/kg was similar to that of randomly selected adults (0.51±0.05g TNT/kg. Conclusion The present animal model could simulate the landmine explosive injuries, and may be used in research of landmine explosive injuries. DOI: 10.11855/j.issn.0577-7402.2014.01.14

  14. Methodology for Determining Optimal Exposure Parameters of a Hyperspectral Scanning Sensor

    Science.gov (United States)

    Walczykowski, P.; Siok, K.; Jenerowicz, A.

    2016-06-01

    The purpose of the presented research was to establish a methodology that would allow the registration of hyperspectral images with a defined spatial resolution on a horizontal plane. The results obtained within this research could then be used to establish the optimum sensor and flight parameters for collecting aerial imagery data using an UAV or other aerial system. The methodology is based on an user-selected optimal camera exposure parameters (i.e. time, gain value) and flight parameters (i.e. altitude, velocity). A push-broom hyperspectral imager- the Headwall MicroHyperspec A-series VNIR was used to conduct this research. The measurement station consisted of the following equipment: a hyperspectral camera MicroHyperspec A-series VNIR, a personal computer with HyperSpec III software, a slider system which guaranteed the stable motion of the sensor system, a white reference panel and a Siemens star, which was used to evaluate the spatial resolution. Hyperspectral images were recorded at different distances between the sensor and the target- from 5m to 100m. During the registration process of each acquired image, many exposure parameters were changed, such as: the aperture value, exposure time and speed of the camera's movement on the slider. Based on all of the registered hyperspectral images, some dependencies between chosen parameters had been developed: - the Ground Sampling Distance - GSD and the distance between the sensor and the target, - the speed of the camera and the distance between the sensor and the target, - the exposure time and the gain value, - the Density Number and the gain value. The developed methodology allowed us to determine the speed and the altitude of an unmanned aerial vehicle on which the sensor would be mounted, ensuring that the registered hyperspectral images have the required spatial resolution.

  15. Determining Spectral Reflectance Coefficients from Hyperspectral Images Obtained from Low Altitudes

    Science.gov (United States)

    Walczykowski, P.; Jenerowicz, A.; Orych, A.; Siok, K.

    2016-06-01

    Remote Sensing plays very important role in many different study fields, like hydrology, crop management, environmental and ecosystem studies. For all mentioned areas of interest different remote sensing and image processing techniques, such as: image classification (object and pixel- based), object identification, change detection, etc. can be applied. Most of this techniques use spectral reflectance coefficients as the basis for the identification and distinction of different objects and materials, e.g. monitoring of vegetation stress, identification of water pollutants, yield identification, etc. Spectral characteristics are usually acquired using discrete methods such as spectrometric measurements in both laboratory and field conditions. Such measurements however can be very time consuming, which has led many international researchers to investigate the reliability and accuracy of using image-based methods. According to published and ongoing studies, in order to acquire these spectral characteristics from images, it is necessary to have hyperspectral data. The presented article describes a series of experiments conducted using the push-broom Headwall MicroHyperspec A-series VNIR. This hyperspectral scanner allows for registration of images with more than 300 spectral channels with a 1.9 nm spectral bandwidth in the 380- 1000 nm range. The aim of these experiments was to establish a methodology for acquiring spectral reflectance characteristics of different forms of land cover using such sensor. All research work was conducted in controlled conditions from low altitudes. Hyperspectral images obtained with this specific type of sensor requires a unique approach in terms of post-processing, especially radiometric correction. Large amounts of acquired imagery data allowed the authors to establish a new post- processing approach. The developed methodology allowed the authors to obtain spectral reflectance coefficients from a hyperspectral sensor mounted on an

  16. DETERMINING SPECTRAL REFLECTANCE COEFFICIENTS FROM HYPERSPECTRAL IMAGES OBTAINED FROM LOW ALTITUDES

    Directory of Open Access Journals (Sweden)

    P. Walczykowski

    2016-06-01

    Full Text Available Remote Sensing plays very important role in many different study fields, like hydrology, crop management, environmental and ecosystem studies. For all mentioned areas of interest different remote sensing and image processing techniques, such as: image classification (object and pixel- based, object identification, change detection, etc. can be applied. Most of this techniques use spectral reflectance coefficients as the basis for the identification and distinction of different objects and materials, e.g. monitoring of vegetation stress, identification of water pollutants, yield identification, etc. Spectral characteristics are usually acquired using discrete methods such as spectrometric measurements in both laboratory and field conditions. Such measurements however can be very time consuming, which has led many international researchers to investigate the reliability and accuracy of using image-based methods. According to published and ongoing studies, in order to acquire these spectral characteristics from images, it is necessary to have hyperspectral data. The presented article describes a series of experiments conducted using the push-broom Headwall MicroHyperspec A-series VNIR. This hyperspectral scanner allows for registration of images with more than 300 spectral channels with a 1.9 nm spectral bandwidth in the 380- 1000 nm range. The aim of these experiments was to establish a methodology for acquiring spectral reflectance characteristics of different forms of land cover using such sensor. All research work was conducted in controlled conditions from low altitudes. Hyperspectral images obtained with this specific type of sensor requires a unique approach in terms of post-processing, especially radiometric correction. Large amounts of acquired imagery data allowed the authors to establish a new post- processing approach. The developed methodology allowed the authors to obtain spectral reflectance coefficients from a hyperspectral sensor

  17. Rigorous Photogrammetric Processing of CHANG'E-1 and CHANG'E-2 Stereo Imagery for Lunar Topographic Mapping

    Science.gov (United States)

    Di, K.; Liu, Y.; Liu, B.; Peng, M.

    2012-07-01

    Chang'E-1(CE-1) and Chang'E-2(CE-2) are the two lunar orbiters of China's lunar exploration program. Topographic mapping using CE-1 and CE-2 images is of great importance for scientific research as well as for preparation of landing and surface operation of Chang'E-3 lunar rover. In this research, we developed rigorous sensor models of CE-1 and CE-2 CCD cameras based on push-broom imaging principle with interior and exterior orientation parameters. Based on the rigorous sensor model, the 3D coordinate of a ground point in lunar body-fixed (LBF) coordinate system can be calculated by space intersection from the image coordinates of con-jugate points in stereo images, and the image coordinates can be calculated from 3D coordinates by back-projection. Due to uncer-tainties of the orbit and the camera, the back-projected image points are different from the measured points. In order to reduce these inconsistencies and improve precision, we proposed two methods to refine the rigorous sensor model: 1) refining EOPs by correcting the attitude angle bias, 2) refining the interior orientation model by calibration of the relative position of the two linear CCD arrays. Experimental results show that the mean back-projection residuals of CE-1 images are reduced to better than 1/100 pixel by method 1 and the mean back-projection residuals of CE-2 images are reduced from over 20 pixels to 0.02 pixel by method 2. Consequently, high precision DEM (Digital Elevation Model) and DOM (Digital Ortho Map) are automatically generated.

  18. Personal exposure to inhalable cement dust among construction workers.

    Science.gov (United States)

    Peters, Susan; Thomassen, Yngvar; Fechter-Rink, Edeltraud; Kromhout, Hans

    2009-01-01

    Objective- A case study was carried out to assess cement dust exposure and its determinants among construction workers and for comparison among workers in cement and concrete production.Methods- Full-shift personal exposure measurements were performed and samples were analysed for inhalable dust and its cement content. Exposure variability was modelled with linear mixed models.Results- Inhalable dust concentrations at the construction site ranged from 0.05 to 34 mg/m(3), with a mean of 1.0 mg/m(3). Average concentration for inhalable cement dust was 0.3 mg/m(3) (GM; range 0.02-17 mg/m(3)). Levels in the ready-mix and pre-cast concrete plants were on average 0.5 mg/m(3) (GM) for inhalable dust and 0.2 mg/m(3) (GM) for inhalable cement dust. Highest concentrations were measured in cement production, particularly during cleaning tasks (inhalable dust GM = 55 mg/m(3); inhalable cement dust GM = 33 mg/m(3)) at which point the workers wore personal protective equipment. Elemental measurements showed highest but very variable cement percentages in the cement plant and very low percentages during reinforcement work and pouring. Most likely other sources were contributing to dust concentrations, particularly at the construction site. Within job groups, temporal variability in exposure concentrations generally outweighed differences in average concentrations between workers. 'Using a broom', 'outdoor wind speed' and 'presence of rain' were overall the most influential factors affecting inhalable (cement) dust exposure.Conclusion- Job type appeared to be the main predictor of exposure to inhalable (cement) dust at the construction site. Inhalable dust concentrations in cement production plants, especially during cleaning tasks, are usually considerably higher than at the construction site.

  19. Identification of microRNAs and their targets in Paulownia fortunei plants free from phytoplasma pathogen after methyl methane sulfonate treatment.

    Science.gov (United States)

    Fan, Guoqiang; Niu, Suyan; Zhao, Zhenli; Deng, Minjie; Xu, Enkai; Wang, Yuanlong; Yang, Lu

    2016-08-01

    MicroRNAs (miRNAs) play major roles in plant responses to various biotic and abiotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. Paulownia witches' broom (PaWB) disease caused by phytoplasmas reduces Paulownia production worldwide. In this study, we investigated the miRNA-mediated plant response to PaWB phytoplasma by Illumina sequencing and degradome analysis of Paulownia fortunei small RNAs (sRNAs). The sRNA and degradome libraries were constructed from healthy and diseased P. fortunei plants and the plants free from phytoplasma pathogen after 60 mg L(-1) methyl methane sulfonate treatment. A total of 96 P. fortunei-conserved miRNAs and 83 putative novel miRNAs were identified. Among them, 37 miRNAs (17 conserved, 20 novel) were found to be differentially expressed in response to PaWB phytoplasma infection. In addition, 114 target genes for 18 of the conserved miRNA families and 33 target genes for 15 of the novel miRNAs in P. fortunei were detected. The expression patterns of 14 of the PaWB phytoplasma-responsive miRNAs and 12 target genes were determined by quantitative real-time polymerase chain reaction (qPCR) experiments. A functional analysis of the miRNA targets indicated that these targeted genes may regulate transcription, stress response, nitrogen metabolism, and various other activities. Our results will help identify the potential roles of miRNAs involved in protecting P. fortunei from diseases.

  20. The CarbonSat candidate mission: imaging greenhouse gas concentrations from space

    Science.gov (United States)

    Sierk, Bernd; Caron, Jerôme; Löscher, Armin; Meijer, Yasjka; Bézy, Jean-Loup; Buchwitz, Michael; Bovensmann, H.

    2014-09-01

    CarbonSat is a proposed Earth observation mission, which was selected in 2010 as one of two candidates for becoming the European Space Agency's (ESA) eighth Earth Explorer (EE8). It is currently undergoing parallel feasibility studies (phase A) performed by two industrial consortia. CarbonSat aims at a better understanding of the natural and anthropogenic sources and sinks of the two most important anthropogenic greenhouse gases CO2 and CH4, which will contribute to a better understanding of climate feedback and forcing mechanisms. To achieve these objectives the instrument will quantify and monitor the spatial distribution of carbon dioxide (CO2) and methane (CH4). It will deliver global data sets of dry air column-averaged mixing ratios of these gases with high precision (1 - 3 ppm for CO2 and 6 - 12 ppb for CH4) and accuracy (0.5 ppm for CO2 and 5 ppb for CH4). The measurements will provide global coverage every 12 days above 40 degrees latitude at a spatial resolution of 2 x 3 km2. The retrieval products are inferred from observations of Earth radiance and solar irradiance at high to medium spectral resolution (0.1-0.55 nm) in the Near Infrared (747-773 nm) and Short Wave Infrared (1590- 1675 nm and 1925-2095 nm) spectral regions. The combination of high spatial resolution and global coverage requires a swath width larger than 180 km for three spatially co-aligned push-broom imaging spectrometers. The targeted product accuracy translates into stringent radiometric, spectral and geometric requirements for the instrument. This paper presents the system requirements derived from the demanding mission objectives and reports preliminary results of the feasibility studies. It highlights the key components of the instrument, focusing on the optical conceptual design, and addresses the identified critical performance aspects.

  1. TcCYS4, a cystatin from cocoa, reduces necrosis triggered by MpNEP2 in tobacco plants.

    Science.gov (United States)

    Santana, L S; Costa, M G C; Pirovani, N M; Almeida, A F; Alvim, F C; Pirovani, C P

    2014-09-26

    In Brazil, most cocoa bean production occurs in Southern Bahia. Witches' broom disease arrived in this area in 1989 and has since caused heavy losses in production. The disease is caused by the basidiomycete fungus Moniliophthora perniciosa, a hemibiotrophic fungus that produces the necrosis and ethylene-inducting protein (MpNEP2) during infection; this protein can activate cysteine proteases and induce programmed cell death. Cysteine proteases can be modulated by cystatin. In this study, we overexpressed TcCYS4, a cocoa cystatin, in tobacco plants and evaluated the effect on MpNEP2 in model plants. Tccys4 cDNA was cloned into the pCAMBIA 1390 vector and inserted into the tobacco plants via Agrobacterium tumefaciens. Transgene expression was analyzed by reverse transcription-quantitative PCR and Western blot analysis. Transcript and protein levels in Tcccys4:tobacco lines were 8.9- and 1.5-fold higher than in wild-type plants (wt). Tcccys4:tobacco lines showed no change in growth compared to wt plants. CO2 net assimilation (A) increased in Tcccys4:tobacco lines compared to wt plants. Only one line showed statistically significant stomatal conductance (gs) and transpiration rate (E) changes. MpNEP2 was infiltered into the foliar mesophyll of Tcccys4:tobacco lines and wt plants, and necrotic lesions were attenuated in lines highly expressing Tccys4. Our results suggest that cocoa cystatin TcCYS4 affects MpNEP2 activity related to the progression of programmed cell death in tobacco plants. This may occur through the action of cystatin to inhibit cysteine proteases activated by MpNEP2 in plant tissues. Further studies are necessary to examine cystatin in the Theobroma cacao-M. perniciosa pathosystem.

  2. Studies on refining properties of kraft bamboo pulp, hardwood pulp and wheat straw pulp%竹浆、阔叶木浆和麦草浆打浆性能的研究

    Institute of Scientific and Technical Information of China (English)

    吴乾斌; 张美云; 夏新兴; 赵琳

    2012-01-01

      对竹浆、阔叶木浆和麦草浆进行了筛分,得出竹浆中R16目纤维和R200目细小纤维组分含量均最高。收集三种浆料的长纤维,进行打浆,得出:麦草纤维细胞壁容易破除,打浆度和保水值较高,纤维本身强度较差;竹纤维难分丝帚化;竹浆中的细小纤维组分容易破裂,吸水润胀。麦草纤维结合力强,成纸的抗张指数和耐破指数较高,撕裂指数和耐折度低;竹纤维成纸的各项物理指标均较高。%  After screened classification of the kraft bamboo pulp, hardwood pulp and wheat straw pulp, the fibers (above 16 mesh) content and fines (below 200 mesh) content of bamboo pulp are both high. Long fibers (over 200 mesh) of three kinds of pulp being refined, the fiber’s wall of wheat straw is removed easily and fiber’s strength is low. Fiber’s wall of bamboo brooms hardly. Fines of bamboo are beaten easily. The binding strength of wheat straw is high, so the tensile index and burst index are high, while the tear index and folding endurance are low. The entire physical index of bamboo fiber is high.

  3. Effects of Retama raetam (Forssk. Webb & Berthel. (Fabaceae on the central nervous system in experimental animals

    Directory of Open Access Journals (Sweden)

    Al-Tubuly Rida A.

    2011-01-01

    Full Text Available Retama raetam (Forssk. Webb & Berthel. (Fabaceae, commonly known as ‘raetam’ or ‘broom bush’, is a desert shrub that grows abundantly in North-African countries, Palestine and Syria. Traditionally, this plant has been used as an abortifacient, a purgative and a vermifuge. In the present study, the effect of the methanol (MeOH extract of the aerial parts of R. raetam on the central nervous system (CNS has been evaluated using a mice model. In the photoelectrical cell test, the extract of R. raetam (ERR at a dose of 125 mg/kg body weight did not exhibit any effect on the spontaneous motor activity in mice. At a dose of 250 mg/kg body weight, ERR increased ambulatory movement, but had no effect on the non-ambulatory movement, while a dose of 375 mg/kg body weight decreased both ambulatory and non-ambulatory movements. The effect of ERR on the anxiety levels and behaviors of mice was investigated using the elevated plus-maze test. At doses of 125, 250 and 375 mg/kg body weight, ERR decreased anxiety levels without showing an effect on the total activity; it did not affect anxiety levels but increased the total activity; it increased anxiety levels and decreased the total activity, respectively. In the diazepam-induced sleep test, ERR increased the onset of sleep without affecting the duration of sleep at the dose of 250 mg/kg body weight. The dose of 375 mg/kg body weight decreased the onset of sleep while increasing the duration of sleep. ERR did not exhibit any effect on the diazepam-induced sleep in the presence of flumazenil or picrotoxin.

  4. Living with genome instability: the adaptation of phytoplasmas todiverse environments of their insect and plant hosts

    Energy Technology Data Exchange (ETDEWEB)

    Bai, Xiaodong; Zhang, Jianhua; Ewing, Adam; Miller, Sally A.; Radek, Agnes; Shevchenko, Dimitriy; Tsukerman, Kiryl; Walunas, Theresa; Lapidus, Alla; Campbell, John W.; Hogenhout Saskia A.

    2006-02-17

    Phytoplasmas (Candidatus Phytoplasma, Class Mollicutes) cause disease in hundreds of economically important plants, and are obligately transmitted by sap-feeding insects of the order Hemiptera, mainly leafhoppers and psyllids. The 706,569-bp chromosome and four plasmids of aster yellows phytoplasma strain witches broom (AY-WB) were sequenced and compared to the onion yellows phytoplasma strain M (OY-M) genome. The phytoplasmas have small repeat-rich genomes. The repeated DNAs are organized into large clusters, potential mobile units (PMUs), which contain tra5 insertion sequences (ISs), and specialized sigma factors and membrane proteins. So far, PMUs are unique to phytoplasmas. Compared to mycoplasmas, phytoplasmas lack several recombination and DNA modification functions, and therefore phytoplasmas probably use different mechanisms of recombination, likely involving PMUs, for the creation of variability, allowing phytoplasmas to adjust to the diverse environments of plants and insects. The irregular GC skews and presence of ISs and large repeated sequences in the AY-WB and OY-M genomes are indicative of high genomic plasticity. Nevertheless, segments of {approx}250 kb, located between genes lplA and glnQ are syntenic between the two phytoplasmas, contain the majority of the metabolic genes and no ISs. AY-WB is further along in the reductive evolution process than OY-M. The AY-WB genome is {approx}154 kb smaller than the OY-M genome, primarily as a result of fewer multicopy sequences, including PMUs. Further, AY-WB lacks genes that are truncated and are part of incomplete pathways in OY-M. This is the first comparative phytoplasma genome analysis and report of the existence of PMUs in phytoplasma genomes.

  5. Authentication of Polygonati Odorati Rhizoma and other two Chinese Materia Medica of the Liliaceae family by pharmacognosy technique with GC-MS analysis.

    Science.gov (United States)

    Liu, Yingjiao; Liu, Canhuang; Yu, Yafei; Xu, Bei; Gong, Limin; Zeng, Xiaoyan; Xiao, Lan; Cheng, Qilai; Liu, Tasi

    2015-02-01

    Yuzhu (Polygonati Odorati Rhizoma), Kangdingyuzhu (Polygonati Prattii Rhizoma), and zhugenqiyuzhu (Disporopsis Fuscopictae Rhizoma) are of the same family, but of different genera. They have all often used in Chinese Materia Medica (CMM) as Polygonati Odorati Rhizoma in China market. Three species of CMM are confused. For better application, we need to identify these plants accurately. This study use pharmacognosy technique and GC-MS analysis, three species of CMM were authenticated. In macroscopic characteristics, the fruit of Polygonati Odorati Rhizoma is blue-black, while the other two are maroon and dark purple orderly. Nodes of Polygonati Odorati Rhizoma are upward and light uplift, about 1 cm spacing, while the other are not. As for microscopic characteristics, the cortex of Polygonati Odorati Rhizoma only occupies about 1/5 of the radius of the transverse section with inconspicuous endodermis, which is much smaller than others. The type of vascular bundles of Polygonati Odorati Rhizoma is closed collateral, but the other is amphivasal. Raphides of calcium oxalate are scattered, but Raphides of the other two are like brooms and neat rows. GC-MS analysis of essential oil could provide different characteristics to distinguish three species. Twenty-three compounds were identified from essential oil of Polygonati Odorati Rhizoma and the main components were n-hexadecanoic acid (49.45%), while n-hexadecanoic acid of the other two are 23.92% and 9.45%. The content of n-hexadecanoic is strongly different. This research was aimed to establish a method by pharmacognosy and GC-MS analysis to identify three CMM and for providing scientifical data to ensure accuracy of origin of three species.

  6. Cytochrome b gene structure and consequences for resistance to Qo inhibitor fungicides in plant pathogens.

    Science.gov (United States)

    Grasso, Valeria; Palermo, Simona; Sierotzki, Helge; Garibaldi, Angelo; Gisi, Ulrich

    2006-06-01

    The cytochrome b (cyt b) gene structure was characterized for different agronomically important plant pathogens, such as Puccinia recondita f sp tritici (Erikss) CO Johnston, P graminis f sp tritici Erikss and Hennings, P striiformis f sp tritici Erikss, P coronata f sp avenae P Syd & Syd, P hordei GH Otth, P recondita f sp secalis Roberge, P sorghi Schwein, P horiana Henn, Uromyces appendiculatus (Pers) Unger, Phakopsora pachyrhizi Syd & P Syd, Hemileia vastatrix Berk & Broome, Alternaria solani Sorauer, A alternata (Fr) Keissl and Plasmopara viticola (Berk & Curt) Berlese & de Toni. The sequenced fragment included the two hot spot regions in which mutations conferring resistance to QoI fungicides may occur. The cyt b gene structure of these pathogens was compared with that of other species from public databases, including the strobilurin-producing fungus Mycena galopoda (Pers) P Kumm, Saccharomyces cerevisiae Meyer ex Hansen, Venturia inaequalis (Cooke) Winter and Mycosphaerella fijiensis Morelet. In all rust species, as well as in A solani, resistance to QoI fungicides caused by the mutation G143A has never been reported. A type I intron was observed directly after the codon for glycine at position 143 in these species. This intron was absent in pathogens such as A alternata, Blumeria graminis (DC) Speer, Pyricularia grisea Sacc, Mycosphaerella graminicola (Fuckel) J Schröt, M fijiensis, V inaequalis and P viticola, in which resistance to QoI fungicides has occurred and the glycine is replaced by alanine at position 143 in the resistant genotype. The present authors predict that a nucleotide substitution in codon 143 would prevent splicing of the intron, leading to a deficient cytochrome b, which is lethal. As a consequence, the evolution of resistance to QoI fungicides based on G143A is not likely to evolve in pathogens carrying an intron directly after this codon.

  7. Impact of eastern dwarf mistletoe (Arceuthobium pusillum) on host white spruce (Picea glauca) development, growth and performance across multiple scales.

    Science.gov (United States)

    Logan, Barry A; Reblin, Jaret S; Zonana, David M; Dunlavey, Ryan F; Hricko, Carolyn R; Hall, Adam W; Schmiege, Stephanie C; Butschek, Ross A; Duran, Kristy L; Emery, R J Neil; Kurepin, Leonid V; Lewis, James D; Pharis, Richard P; Phillips, Nathan G; Tissue, David T

    2013-04-01

    Infection by eastern dwarf mistletoe (Arceuthobium pusillum) modifies needle and branch morphology and hastens white spruce (Picea glauca) mortality. We examined potential causal mechanisms and assessed the impacts of infection-induced alterations to host development and performance across scales ranging from needle hormone contents to bole expansion. Needles on infected branches (IBs) possessed higher total cytokinin (CK) and lower abscisic acid contents than needles on uninfected branches (UBs). IBs exhibited greater xylem growth than same-aged UBs, which is consistent with the promotive effect of CKs on vascular differentiation and organ sink strength. Elevated CK content may also explain the dense secondary and tertiary branching observed at the site of infection, i.e. the formation of 'witches' brooms' with significantly lower light capture efficiencies. Observed hormone perturbations were consistent with higher rates of transpiration, lower water use efficiencies (WUEs) and more negative needle carbon isotope ratios observed for IBs. Observed reductions in needle size allowed IBs to compensate for reduced hydraulic conductivity. Severe infections resulted in dramatically decreased diameter growth of the bole. It seems likely that the modifications to host hormone contents by eastern dwarf mistletoe infection led white spruce trees to dedicate a disproportionate fraction of their photoassimilate and other resources to self-shaded branches with low WUE. This would have decreased the potential for fixed carbon accumulation, generating a decline in the whole-tree resource pool. As mistletoe infections grew in size and the number of IBs increased, this burden was manifested as increasingly greater reductions in bole growth.

  8. Factors predicting a child's dental fear.

    Science.gov (United States)

    Majstorović, M; Skrinjarić, I; Glavina, D; Szirovicza, L

    2001-12-01

    The aim of the present study was to determine and assess the variables most involved in the etiology of a child's dental fear. The study was performed on a sample of 89 children aged from 5.5 to 12.5 years and their mothers. The sample comprised 37 children with experience of dental trauma (19 boys and 18 girls) and 52 children without experience of dental trauma (28 boys and 24 girls). Corah Dental Anxiety Scale (DAS) was applied to evaluate the level of the child's (CDAS) and mother's (MDAS) dental anxiety. Broome's Child Medical Fear Questionnaire (CMFQ) was used to assess the child's fear of medical treatment. Hollingshead Two Factor Index of Social Position (ISP) was calculated to assess socio-economic status of the family. Cluster analysis differentiated one group of dentally anxious children with the highest level of maternal anxiety (MDAS = 14.44) and the lowest socio-economic status (ISP = 41.94). Another group of extremely anxious children (CDAS = 14.31) showed the highest fear of medical treatment (CMFQ = 22.08) and rather low socio-economic status. One group represented children with the lowest CDAS (5.63), lowest MDAS (8.46), and lowest CMFQ (13.54). Linear regression analysis showed high correlation between previous traumatic medical experiences and a child's dental anxiety using the linear model CDAS' = b0 + b1 x CMFQ. The analysis revealed that a child's dental fear mostly depends on early negative medical experience, while material dental anxiety and socio-economic circumstances seem to be of less importance.

  9. Atividade de enzimas associadas ao estado de indução em mudas de cacaueiro expostas a dois actinomicetos residentes de filoplano Activity of enzymes associates of induced resistance on cocoa seedlings exposed of two actinomycetes phylloplane residents

    Directory of Open Access Journals (Sweden)

    Dirceu Macagnan

    2008-02-01

    Full Text Available Dois antagonistas selecionados para o biocontrole da vassoura-de-bruxa do cacaueiro foram avaliados quanto à capacidade em ativar mecanismos de defesa de plantas contra patógenos. Para tanto, mudas seminais de cacaueiro "comum" foram cultivadas em casa-de-vegetação por 30 dias e expostas aos antagonistas aplicados a mudas de cacaueiro por atomização, individualmente e em associação. O primeiro par de folhas das mudas dos diferentes tratamentos foi coletado aos dois, quatro, 12 e 24 dias após a exposição aos antagonistas. Foi quantificada a atividade de peroxidases, polifenoloxidases, quitinases e beta-1,3-glucanases no material coletado. Observou-se um aumento na atividade de peroxidases e polifenoloxidases nos primeiros dias após a exposição das mudas, especialmente ao isolado Ac26. Não foi observado efeito aditivo ou sinergístico nas mudas expostas aos dois isolados simultaneamente.Two antagonists selected for the biocontrol of cocoa witches' broom were investigated for their ability in triggering increases in the activity of enzymes associated to induced resistance. In a greenhouse, thirty days old cocoa seedlings were exposed t antagonists by spraying a propagule suspension of every antagonist or a mixture of them. At two, four 12 and 24 days exposing plants to the antagonists, the first leaf pair of every plant was excised and used for quantifying the activity of peroxidases, poly-phenol-oxidases, chitinases and beta-1,3-glucanases. There were increases in activity of peroxidases, poly-phenol-oxidases, mainly in the case of isolate Ac26. Additive or synergistic effects were not observed as a consequence of exposing plants to both antagonists together.

  10. Apple proliferation phytoplasma influences the pattern of plant volatiles emitted depending on pathogen virulence

    Directory of Open Access Journals (Sweden)

    Margit eRid

    2016-01-01

    Full Text Available Apple proliferation (AP and pear decline (PD are the most severe diseases in pome fruit growing areas. AP-infected trees show typical symptoms such as witches’ broom, enlarged stipules, tasteless and dwarf fruits. PD-infected pears show a progressive weakening, reduced terminal growth, smaller fruits and die within weeks (quick decline or years (slow decline. The diseases are caused by the cell-wall lacking bacteria Candidatus Phytoplasma mali (AP phytoplasma and Ca. P. pyri (PD phytoplasma, respectively. In previous studies it has been shown that AP-infected apple trees emitted higher amounts of the sesquiterpene β-caryophyllene, an attractant of the insect vector Cacopsylla picta (Hemiptera: Psyllidae, thereby facilitating the dispersal of AP phytoplasma. In the present study, volatile organic compounds (VOCs occurring in the headspace of plants infected with Ca. P. mali strains causing different severity of symptoms in apple plants were collected, analyzed and identified. Headspace samples from healthy and AP-infected model plant tobacco (Nicotiana occidentalis and apple (Malus domestica as well as from healthy and PD-infected pear (Pyrus communis were investigated via thermodesorption and GC-MS analysis. Significantly higher concentrations of ethyl benzoate were produced in all phytoplasma-infected plants compared to healthy ones and an as yet unidentified sesquiterpene differed between the odor bouquets of healthy and by Ca. P. mali infected tobacco plants. Additionally, statistically significant higher amounts of both compounds were measured in the headspace of plants infected by the virulent AP strain. In apple, significantly higher concentrations of ethyl benzoate and methyl salicylate were observed for trees infected with strains of Ca. P. mali. Ethyl benzoate was also detected in the headspace of pear trees infected with Ca. P. pyri.

  11. The detection of heterozygous familial hypercholesterolemia in Ireland.

    LENUS (Irish Health Repository)

    O'Kane, Maurice J

    2012-05-01

    Heterozygous familial hypercholesterolemia (HeFH) is an autosomal dominant condition with a population prevalence of 1 in 500, and is associated with significant cardiovascular morbidity and mortality. It may be caused by mutations in the low-density lipoprotein (LDL) receptor, apolipoprotein B100 (Apo B100), or proprotein convertase subtilisin\\/kexin type 9 (PCSK9) genes, with over 1,000 causative mutations described. Statin therapy in HeFH is considered effective and safe. Audit data suggest that approximately 80% of the putative HeFH population remains unidentified and, therefore, there is a need to develop a strategy for the identification of affected individuals so that early lipid-lowering treatment may be offered. There is good evidence showing the effectiveness and acceptability of HeFH screening programs in Europe. The authors describe a protocol for an all island approach to HeFH detection in the Republic of Ireland\\/Northern Ireland. Index cases will be identified by opportunistic screening using the Simon Broome, or Make Early Diagnosis to Prevent Early Death (MedPed) and World Health Organization (WHO) criteria. Patients identified as "definite," "probable," or "possible" HeFH criteria will be offered genetic testing. The authors expect causative mutations to be identified in approximately 80% of patients with "definite" HeFH but in only approximately 20% of patients with "possible" HeFH. Cascade screening will be undertaken in first-degree relatives of the index case using genetic testing (where a causative mutation has been identified), or otherwise using LDL cholesterol concentration. The establishment of a HeFH screening program on an all-island basis will require: expansion of the existing molecular genetics diagnostic services, the establishment of a cohort of nurses\\/genetic counselors, a HeFH database to support cascade testing, the development of a network of lipid clinics (in a primary or secondary care setting), and an educational

  12. The High Resolution Stereo Camera (HRSC) of Mars Express and its approach to science analysis and mapping for Mars and its satellites

    Science.gov (United States)

    Gwinner, K.; Jaumann, R.; Hauber, E.; Hoffmann, H.; Heipke, C.; Oberst, J.; Neukum, G.; Ansan, V.; Bostelmann, J.; Dumke, A.; Elgner, S.; Erkeling, G.; Fueten, F.; Hiesinger, H.; Hoekzema, N. M.; Kersten, E.; Loizeau, D.; Matz, K.-D.; McGuire, P. C.; Mertens, V.; Michael, G.; Pasewaldt, A.; Pinet, P.; Preusker, F.; Reiss, D.; Roatsch, T.; Schmidt, R.; Scholten, F.; Spiegel, M.; Stesky, R.; Tirsch, D.; van Gasselt, S.; Walter, S.; Wählisch, M.; Willner, K.

    2016-07-01

    The High Resolution Stereo Camera (HRSC) of ESA's Mars Express is designed to map and investigate the topography of Mars. The camera, in particular its Super Resolution Channel (SRC), also obtains images of Phobos and Deimos on a regular basis. As HRSC is a push broom scanning instrument with nine CCD line detectors mounted in parallel, its unique feature is the ability to obtain along-track stereo images and four colors during a single orbital pass. The sub-pixel accuracy of 3D points derived from stereo analysis allows producing DTMs with grid size of up to 50 m and height accuracy on the order of one image ground pixel and better, as well as corresponding orthoimages. Such data products have been produced systematically for approximately 40% of the surface of Mars so far, while global shape models and a near-global orthoimage mosaic could be produced for Phobos. HRSC is also unique because it bridges between laser altimetry and topography data derived from other stereo imaging instruments, and provides geodetic reference data and geological context to a variety of non-stereo datasets. This paper, in addition to an overview of the status and evolution of the experiment, provides a review of relevant methods applied for 3D reconstruction and mapping, and respective achievements. We will also review the methodology of specific approaches to science analysis based on joint analysis of DTM and orthoimage information, or benefitting from high accuracy of co-registration between multiple datasets, such as studies using multi-temporal or multi-angular observations, from the fields of geomorphology, structural geology, compositional mapping, and atmospheric science. Related exemplary results from analysis of HRSC data will be discussed. After 10 years of operation, HRSC covered about 70% of the surface by panchromatic images at 10-20 m/pixel, and about 97% at better than 100 m/pixel. As the areas with contiguous coverage by stereo data are increasingly abundant, we also

  13. Identification and Analysis of Proteins in Latex Lutoid Associated with Tapping Panel Dryness of Hevea brasiliensis%橡胶树胶乳黄色体死皮相关蛋白的鉴定及分析

    Institute of Scientific and Technical Information of China (English)

    周雪梅; 杨礼富; 王真辉; 邹智; 袁坤

    2012-01-01

    本实验通过双向凝胶电泳技术(2-DE)比较橡胶死皮树与健康树胶乳黄色体蛋白表达谱的差异,通过软件分析比较获得37个差异表达的蛋白点,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后,搜索NCBInr数据库,结果表明,有11个蛋白点被成功鉴定,其中7个为未知蛋白,其余4个蛋白分别为chloroplast 23 kDa polypeptide of photosystem Ⅱ、ATP synthse beta chain、NADP-dependent sorbitol-6-phosphate dehydrogenase和vitamin-b12 independent methionine synthase.这4个蛋白在死皮树中表达量均下调,可能在死皮发生过程中起着重要作用.本研究将为进一步阐明橡胶树死发发生的分子机制提供理论依据.%In this experiment, the two-dimensional gel electrophoresis(2-DE) was used to study the differentiation of protein expression profiles of latex lutoid between TPD trees and healthy ones in Hevea brasiliatsis. 37 differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry ( MALDI-TOF MS) followed by searching for NCBInr database, the result showed that 11 spots were successfully identified, of which 7 were identified as the unknown proteins, and the remaining 4 were the chloroplast 23 kDa polypeptide of photosystem Ⅱ, ATP synthase beta chain, NADP-depend-ent sorbitol-6-phosphate dehydrogenase and vitamin-bl2 independent methionine synthase. There were some relationship between osmotin protein and TPD broom according to the functions of down-regulated proteins in TPD trees. This research provided a theoretical basis for TPD.

  14. Data Requirements For The Water Directive - Role of Remote Sensing Using The Hrsc-ax Camera Illustrated By The Blue City Project

    Science.gov (United States)

    Martin, J.; O'Kane, J. P.

    A completely digital, aerial survey of Cork City and the Lee Catchment, flown in May 2001 at an altitude of 3000m, covered an area of 325sqkm. The campaign used the German Aerospace Centres HRSC-AX system. The SAXT (Airborne, Extended- & cedil;Generation) version of the HRSC (High Resolution Stereo Camera), in commercial operation since late 2000, produces very high resolution Digital Elevation Models (DEMs) and multi-spectral ortho-rectified images. The system contains nine CCD line sensors, mounted in parallel behind one single optic. Operating on a push-broom principle, these acquire nine superimposed image tracks simultaneously (along-track). Five of the nine CCD lines are panchromatic sensors arranged at specific viewing an- gles to provide the multiple-stereo and photogrammetric capabilities of the instrument. The other four are covered with colour filters (red, green, blue near infrared) to ac- quire multi-spectral images. The fully automatic photogrammetric and cartographic processing system, developed at the DLR Institute of Space Sensor Technology and Planetary Exploration (in co-operation with the Technical University of Berlin) yields mean absolute accuracies of s15-20cm in both the horizontal and vertical directions ´ for all products. For the Blue City Project, the system yielded a DEM with a resolu- tion of 100cm, a nadir (panchromatic) ortho-mosaic with a 15cm resolution, and true colour (R, G, B and nIR) ortho-mosaics with a 30cm resolution. Joining the colour bands yielded true-colour (RGB) and false-colour (nIR,G,B) ortho-image mosaics of the full area. The combination of these high resolution products allows detailed to- pographic and multi-spectral analysis of the study area. This data set will provide the foundation for a multi-purpose geographic information system, linked to hydro- dynamic, hydraulic and hydrologic computer models of surface and subsurface flow, including water quality, throughout the catchment of the Lee and the City of

  15. A genetically anchored physical framework for Theobroma cacao cv. Matina 1-6

    Directory of Open Access Journals (Sweden)

    Kuhn David N

    2011-08-01

    Full Text Available Abstract Background The fermented dried seeds of Theobroma cacao (cacao tree are the main ingredient in chocolate. World cocoa production was estimated to be 3 million tons in 2010 with an annual estimated average growth rate of 2.2%. The cacao bean production industry is currently under threat from a rise in fungal diseases including black pod, frosty pod, and witches' broom. In order to address these issues, genome-sequencing efforts have been initiated recently to facilitate identification of genetic markers and genes that could be utilized to accelerate the release of robust T. cacao cultivars. However, problems inherent with assembly and resolution of distal regions of complex eukaryotic genomes, such as gaps, chimeric joins, and unresolvable repeat-induced compressions, have been unavoidably encountered with the sequencing strategies selected. Results Here, we describe the construction of a BAC-based integrated genetic-physical map of the T. cacao cultivar Matina 1-6 which is designed to augment and enhance these sequencing efforts. Three BAC libraries, each comprised of 10× coverage, were constructed and fingerprinted. 230 genetic markers from a high-resolution genetic recombination map and 96 Arabidopsis-derived conserved ortholog set (COS II markers were anchored using pooled overgo hybridization. A dense tile path consisting of 29,383 BACs was selected and end-sequenced. The physical map consists of 154 contigs and 4,268 singletons. Forty-nine contigs are genetically anchored and ordered to chromosomes for a total span of 307.2 Mbp. The unanchored contigs (105 span 67.4 Mbp and therefore the estimated genome size of T. cacao is 374.6 Mbp. A comparative analysis with A. thaliana, V. vinifera, and P. trichocarpa suggests that comparisons of the genome assemblies of these distantly related species could provide insights into genome structure, evolutionary history, conservation of functional sites, and improvements in physical map

  16. How big is an OMI pixel?

    Science.gov (United States)

    de Graaf, Martin; Sihler, Holger; Tilstra, Lieuwe G.; Stammes, Piet

    2016-08-01

    The Ozone Monitoring Instrument (OMI) is a push-broom imaging spectrometer, observing solar radiation backscattered by the Earth's atmosphere and surface. The incoming radiation is detected using a static imaging CCD (charge-coupled device) detector array with no moving parts, as opposed to most of the previous satellite spectrometers, which used a moving mirror to scan the Earth in the across-track direction. The field of view (FoV) of detector pixels is the solid angle from which radiation is observed, averaged over the integration time of a measurement. The OMI FoV is not quadrangular, which is common for scanning instruments, but rather super-Gaussian shaped and overlapping with the FoV of neighbouring pixels. This has consequences for pixel-area-dependent applications, like cloud fraction products, and visualisation.The shapes and sizes of OMI FoVs were determined pre-flight by theoretical and experimental tests but never verified after launch. In this paper the OMI FoV is characterised using collocated MODerate resolution Imaging Spectroradiometer (MODIS) reflectance measurements. MODIS measurements have a much higher spatial resolution than OMI measurements and spectrally overlap at 469 nm. The OMI FoV was verified by finding the highest correlation between MODIS and OMI reflectances in cloud-free scenes, assuming a 2-D super-Gaussian function with varying size and shape to represent the OMI FoV. Our results show that the OMPIXCOR product 75FoV corner coordinates are accurate as the full width at half maximum (FWHM) of a super-Gaussian FoV model when this function is assumed. The softness of the function edges, modelled by the super-Gaussian exponents, is different in both directions and is view angle dependent.The optimal overlap function between OMI and MODIS reflectances is scene dependent and highly dependent on time differences between overpasses, especially with clouds in the scene. For partially clouded scenes, the optimal overlap function was

  17. Evolution of foredune barriers at Admiral Bay, Western Australia - Implications for Holocene relative sea levels and extreme wave events

    Science.gov (United States)

    Engel, Max; May, Simon Matthias; Scheffers, Anja; Squire, Peter; Pint, Anna; Kelletat, Dieter; Brückner, Helmut

    2014-05-01

    Only few geomorphological studies on the Canning Coast of Western Australia exist to date, most probably reflecting its remoteness and low population density. However, WA's annual gross state product (GSP) growth of ~5 % during the past decade and the highest GSP per capita nationwide resulting from a mining boom increase public attention as well as the demand for precise information on landscape inventory and evolution. In this paper, new data from a sequence of vegetated foredune barriers, gradually being eroded by a migrating estuary inside the macrotidal Admiral Bay (also known as McKelson Creek, Whistle Creek or Panganunganyjal), 110 km southwest of Broome, are presented. Based on sediment cores, DGPS-based elevation transects, and stratigraphical analyses on outcrops of the relict foredunes, we aim at (i) reconstructing lateral coastal changes during the Holocene, (ii) drawing inferences on relative sea-level (RSL) change, and (iii) identifying and dating imprints of extreme-wave events. Sedimentary analyses comprise documentation of bedding structures, foraminiferal content and macrofaunal remains (including shell taphonomy), grain size, and organic matter. Chronological contexts are established using 26 14C-AMS datings. Marine flooding of the pre-Holocene base landward of the 2.5 km-wide foredunes can be pinpointed to 7400-7200 cal BP. A mangrove ecosystem established and was quickly replaced by intertidal coarse sands after only 200-400 years. The high-energy intertidal environment prevailed until c. 4000 cal BP before turning into the present supralittoral mudflat environment. At that time, coastal regression led to beach progradation and isochronic formation of foredune barriers. Drivers of progradation were a stable RSL or gradual RSL fall after the mid-Holocene highstand and a positive sand budget provided by high sublittoral productivity of calcareous shells in combination with erosion at the adjacent sandstone capes and longshore drift. The foredunes

  18. Multiband CMOS sensor simplify FPA design

    Science.gov (United States)

    Wang, Weng Lyang B.; Ling, Jer

    2015-10-01

    Push broom multi-band Focal Plane Array (FPA) design needs to consider optics, image sensor, electronic, mechanic as well as thermal. Conventional FPA use two or several CCD device as an image sensor. The CCD image sensor requires several high speed, high voltage and high current clock drivers as well as analog video processors to support their operation. Signal needs to digitize using external sample / hold and digitized circuit. These support circuits are bulky, consume a lot of power, must be shielded and placed in close to the CCD to minimize the introduction of unwanted noise. The CCD also needs to consider how to dissipate power. The end result is a very complicated FPA and hard to make due to more weighs and draws more power requiring complex heat transfer mechanisms. In this paper, we integrate microelectronic technology and multi-layer soft / hard Printed Circuit Board (PCB) technology to design electronic portion. Since its simplicity and integration, the optics, mechanic, structure and thermal design will become very simple. The whole FPA assembly and dis-assembly reduced to a few days. A multi-band CMOS Sensor (dedicated as C468) was used for this design. The CMOS Sensor, allow for the incorporation of clock drivers, timing generators, signal processing and digitization onto the same Integrated Circuit (IC) as the image sensor arrays. This keeps noise to a minimum while providing high functionality at reasonable power levels. The C468 is a first Multiple System-On-Chip (MSOC) IC. This device used our proprietary wafer butting technology and MSOC technology to combine five long sensor arrays into a size of 120 mm x 23.2 mm and 155 mm x 60 mm for chip and package, respectively. The device composed of one Panchromatic (PAN) and four different Multi- Spectral (MS) sensors. Due to its integration on the electronic design, a lot of room is clear for the thermal design. The optical and mechanical design is become very straight forward. The flight model FPA

  19. Personal breathing zone exposures among hot-mix asphalt paving workers; preliminary analysis for trends and analysis of work practices that resulted in the highest exposure concentrations.

    Science.gov (United States)

    Osborn, Linda V; Snawder, John E; Kriech, Anthony J; Cavallari, Jennifer M; McClean, Michael D; Herrick, Robert F; Blackburn, Gary R; Olsen, Larry D

    2013-01-01

    these exposures and the use of diesel oil, use of a diesel-powered screed, elevated HMA paving application temperatures, lubricating and working on broken-down equipment, and operation of a broom machine.

  20. Reversing land degradation through grasses: a systematic meta-analysis in the Indian tropics

    Science.gov (United States)

    Mandal, Debashis; Srivastava, Pankaj; Giri, Nishita; Kaushal, Rajesh; Cerda, Artemi; Meherul Alam, Nurnabi

    2017-02-01

    Although intensive agriculture is necessary to sustain the world's growing population, accelerated soil erosion contributes to a decrease in the environmental health of ecosystems at local, regional and global scales. Reversing the process of land degradation using vegetative measures is of utmost importance in such ecosystems. The present study critically analyzes the effect of grasses in reversing the process of land degradation using a systematic review. The collected information was segregated under three different land use and land management situations. Meta-analysis was applied to test the hypothesis that the use of grasses reduces runoff and soil erosion. The effect of grasses was deduced for grass strip and in combination with physical structures. Similarly, the effects of grasses were analyzed in degraded pasture lands. The overall result of the meta-analysis showed that infiltration capacity increased approximately 2-fold after planting grasses across the slopes in agricultural fields. Grazing land management through a cut-and-carry system increased conservation efficiencies by 42 and 63 % with respect to reduction in runoff and erosion, respectively. Considering the comprehensive performance index (CPI), it has been observed that hybrid Napier (Pennisetum purpureum) and sambuta (Saccharum munja) grass seem to posses the most desirable attributes as an effective grass barrier for the western Himalayas and Eastern Ghats, while natural grass (Dichanthium annulatum) and broom grass (Thysanolaena maxima) are found to be most promising grass species for the Konkan region of the Western Ghats and the northeastern Himalayan region, respectively. In addition to these benefits, it was also observed that soil carbon loss can be reduced by 83 % with the use of grasses. Overall, efficacy for erosion control of various grasses was more than 60 %; hence, their selection should be based on the production potential of these grasses under given edaphic and agro

  1. Transformation of Agrobacteria tumefaciens on healthy and infectious Paulownia%根癌农杆菌对健康和患丛枝病泡桐的遗传转化

    Institute of Scientific and Technical Information of China (English)

    王瑶; 柳晟; 杜涛; 林木兰

    2001-01-01

    Healthy and infectious (infected by Paulownia Witches Broom) Paulownia was selected as the investigated material.It was appropriate for two kinds of Paulownia to select stem segments as transplant,differentiate on MS+BA 4 mg/L+NAA 0.2 mg/L and induce root on 1/2 MS+KT 0.5 mg/L+IBA 0.25 mg/L,the differentiation efficiency could up to 22.8%.Stem segments were infected with Agrobacteria tumefaciens LBA4404 containing plant expression vector p438PRSI,and co-cultured for three days.Resistant shoots were obtained about 20 days on MS media supplemented with kanamycin 50 mg/L.Regenerated plants were grown up after shoot culture and root inducing.PCR and Southern analysis of the transformants revealed that foreign gene has been integrated into plant genomes.Expression of report gene NPTⅡ was confirmed in regenerated plants.The factors influencing transfor mation of Paulownia were also discussed.%选取健康及患丛枝病泡桐(Paulownia spp.)为材料,建立组织培养和植株再生系统,以茎段作为转化受体,诱导分化和生根的最佳激素组合分别是MS+BA 4 mg/L+NAA 0.2 mg/L和1/2 MS+KT 0.5 mg/L+IBA 0.25 mg/L,芽分化频率可达22.8%。健康和患病泡桐的茎段经农杆菌共培养3 d后,在附加50 mg/L Km的选择分化培养基上培养20 d左右再生出抗性芽,经培养、诱导生根,获得了转基因再生植株,建立了泡桐的遗传转化体系。PCR和Southern杂交检测证明外源基因已整合到泡桐的基因组,标记基因ITPⅡ在再生植株中也得到表达。同时对影响转化的一些因素进行了探讨。

  2. Observaciones sobre características, distribución y daños de sinfilidos (Symphyla y otros organismos del suelo en cultivos de piña, Ananas comosus, del Valle

    Directory of Open Access Journals (Sweden)

    Zuluaga C. José Iván

    1988-12-01

    Full Text Available Como en los últimos años los cultivos de piña, Ananas comosus, del Valle, han sido atacados por sinfílifos, clasificados por el Dr. Evert Lindquist como Scutigerella inmaculata, se estudiaron aspectos biológicos, distribución, daños y relaciones con algunos microorganismos. Mediante encuestas e inspecciones al suelo y plantas se verificó su presencia en Palmira, Pradera, Buga, Buga la grande, Sevilla, Dagua y Darién, principales zonas productoras de piña en este departamento. Ensayos en laboratorio demostraron Que S. inmaculata es de hábitos alimenticios saprófagos y/o fitófagos y de esta forma puede producir daños a las raíces de la pila, causando el síntoma de "escoba", enrojecimiento al follaje y debilidad en la planta; también se demostró que 3 sinfílidos/plante producen los síntomas del ataque. El sinfílido se presenta desde semillero, siendo los ataques tempranos los más graves, causando pérdidas en raíces del 66% aproximadamente.Pineapples culture Ananas comosus of Valle, they have been attacked by symphylids last vean (Symphyla, classified by Dr. Evert Lindquist like Scutigerella inmaculata. Biologie aspects, distribution, harms and relationships with some microorganisms some studied in this work. This has a wide distribution on Valle and through surveys and inspections over soil and plants their presance was verified in Palmira, Pradera, Buga; Bugalagrande, Sevilla, Dagua y Darién, the main producer pineapple zones in this department. Laboratory essays showed that this symphilids has saphrophytes and plant eater food habits and by this way could produce harm the pineapples roots causing symptom "broom", foliage getting red color and weakness in plant; also is shown that three symphylids per plant produce tipical symptom of attacking. It is made evident from seed bed being early attacks were more grave causing' root losses about 66% (percent aproximatilly.

  3. Diversidade genética entre acessos de cacau de fazendas e de banco de germoplasma na Bahia Genetic diversity among farm and germplasm bank accessions of cacao in Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Jeiza Botelho Leal

    2008-07-01

    Full Text Available O objetivo deste trabalho foi avaliar a diversidade genética de acessos de cacau, selecionados previamente como produtivos e resistentes à vassoura-de-bruxa na Bahia, e estudar suas inter-relações com genótipos no banco de germoplasma. Amostras de DNA de folhas dos 120 acessos, coletados em 17 fazendas de sete municípios do Sul da Bahia, foram amplificadas pela técnica de RAPD ("random amplified polymorphic DNA". Os coeficientes de dissimilaridade genética, calculados pelo método de Jaccard a partir das bandas RAPD, permitiram evidenciar, pela análise de agrupamento, que a maioria das seleções das fazendas (89,2% agrupou-se com acessos do banco de germoplasma considerados representativos da diversidade de cacau (híbridos, trinitários, Scavinas, amazônicos e cacau-comum. As demais seleções distribuíram-se em outros sete grupos distintos. Há elevada diversidade genética entre as seleções das fazendas, e algumas delas devem ter-se originado de genitores não incluídos nesta análise. Esses materiais apresentam potencial para seleção de clones com maior diversidade para novos cruzamentos ou uso pelos agricultores.The objective of this work was to assess genetic diversity of farm cacao accessions, previously selected as productive and resistant to witch broom in Bahia, Brazil, and to study their interrelationship with reference genotypes on germplasm bank. DNA samples from 120 cacao accessions, collected on 17 farms in seven counties in Southern Bahia, were amplified by RAPD technique (random amplified polymorphic DNA. The coefficients of genetic dissimilarity, calculated from the RAPD bands by Jaccard method, allowed to show by cluster analysis that most farm selections (89.2% grouped with accessions from germplasm bank considered representatives of the cacao genetic diversity (hybrids, Trinitarian, Scavina, Amazon and common cacao. The other selections were distributed in seven distinct groups. There is high genetic

  4. Global Change Observation Mission (GCOM)

    Science.gov (United States)

    Shimoda, Haruhisa

    . In order to measure aerosols over both ocean and land, it will have a near ultra violet channel, as well as polarization and bi-directional observation capability. The instrument will be composed of several components. They are VNR (visible and near infrared), polarization (POL), and IRS (short wave to long wave infrared (SWI TMI). The VNR and POL will adopt push broom scanners, while IRS will use a conventional whisk broom scanner. VNR is composed of 3 cameras. They have rather small FOVs and cover total of 70 degrees with 3 cameras. VNR is an 11 channel scanner. POL will have two spectral channels, while each spectral channel is composed of 3 polarizations. SWI will have 4 channels. TMI will have 2 split window channels. For, coastal zone and land observation, the IFOV of SGLI for these targets will be around 250m. All channels of VNR except 763 nm and 1.64 m channel of SWI have 250m IFOV. TMI channels have 250m IFOV and all the other channels have 1000m IFOV. There are several options on the orbit. The baseline option is 700km afternoon orbit for GCOM-W1 and 800km morning orbit for GCOM-C1 to continue the AMSR-E observation and GLI observation. Now, it is decided that GCOM-W1 will be in A-Train. By getting into A-train, cross calibration with AMSR-E will be very easy, and there could be many new products with other A-Train sensors. However, if AMSR-E will be operated a long time after GCOM-W1 launch, this orbit will miss observation frequency. Both satellites are medium sized spacecraft, i.e. 1.9 to 2.0 tons.

  5. Incidência de pragas e doenças em agroecossistemas de café orgânico de agricultores familiares em Poço Fundo-MG Incidence of plagues and diseases in agroecossystems of organic coffee of familiar farmers in Poço Fundo-MG

    Directory of Open Access Journals (Sweden)

    Márcia Martins

    2004-12-01

    Full Text Available Objetivando-se determinar a incidência de pragas Leucoptera coffeella (GUÉRIN-MENÉVILLE, 1842 e Hypothenemus hampei (FERRARI, 1867 e doenças Hemileia vastatrix (BERK e BROOME, 1869 e Cercospora coffeicola (BERK e CURTIS, 1880, durante o período de janeiro de 2001 a dezembro de 2002, foram selecionados três agroecossistemas com produção de café orgânico conduzidos por agricultores familiares do município de Poço Fundo-MG. O agroecossistema I possui 2.200 pés da cultivar 'Catuaí Vermelho' em espaçamento 2,5 x 1,3 m (0,72 ha. O agroecossistema II possui 2.000 pés ('Catuaí Vermelho' em espaçamento 2,8 x 1,0 m (0,56 ha. O agroecossistema III possui 1.100 pés ('Icatu Amarelo' em espaçamento 3,0 x 0,9 m (0,36 ha. A determinação da incidência de L. coffeella, C. coffeicola e H. vastatrix foi realizada mediante levantamentos mensais. A determinação da infecção por C. coffeicola em frutos foi realizada nos meses de abril, maio e junho de 2001 e 2002. O levantamento da infestação por H. hampei foi realizado nos meses de janeiro a junho/julho de cada ano. Determinou-se que a infestação por L. coffeella ultrapassou 20% no terço superior (principalmente no período seco. A infestação por H. hampei atingiu o nível de dano somente no agroecossistema I, em 2001, e no agroecossistema III, em 2002. A infecção por H. vastatrix no agroecossistema III não atingiu nível de dano em conseqüência da tolerância da cultivar ('Icatu' à infecção por esse fungo; porém, nos agroecossistemas I e II ('Catuaí Vermelho', a infecção na lavoura ultrapassou o nível de 10% (principalmente no período seco. A infecção por C. coffeicola em folhas e frutos atingiu níveis elevados (período seco. A produtividade do agroecossistema I, em 2001, foi de 510 Kg ha-1 e, em 2002, de 2.340 Kg ha-1; no agroecossistema II, em 2001, foi de 420 Kg ha-1 e, em 2002, de 1.290 Kg ha-1; e, no agroecossistema III, foi praticamente zero, em 2001, e em

  6. Effects of Agrobacterium tumefac iens on the Symptoms of Paulownia sp. Plantlet in Vitro Cultured%根癌农杆菌对感染植原体的泡桐组培苗症状的影响

    Institute of Scientific and Technical Information of China (English)

    田国忠; 朱水芳; 罗飞; 李怀方; 裘维蕃

    2001-01-01

    采用含有激素合成相关基因的根癌农杆菌,伤口接种已感染植原体的泡桐丛枝组培苗和健康组培苗,结果发现对丛枝苗的致瘤能力明显低于健康对照苗,且被接种病苗的丛枝症状缓解.从健苗获得的T-DNA转化泡桐瘤组织细胞能在无激素培养基上稳定生长和连续继代培养2年以上,说明瘤组织细胞自身已获得了细胞分裂素和生长素合成能力.根据已报道的根癌农杆菌株系pTil 5955 T-DNA的异戊烯基转移酶基因(ipt)的保守序列,设计了一对引物(CYT和CYT′),用多聚酶链式反应(PCR)扩增了我国杨树致瘤农杆菌ipt基因部分序列(427 bp片段),也从遗传转化的两个泡桐无性系瘤组织At-ZH和At-T35扩增出此特异片段,从而进一步肯定了T-DNA已被整合到泡桐的染色体上,表明泡桐易于通过Ti质粒载体途径进行基因转移操作,但用此引物未能从泡桐、甘薯健株和感染植原体的组培病苗扩增出相应的427 bp特异片段.当用此遗传转化瘤组织嫁接病苗时,可减轻丛枝症状的严重度,延长病苗的存活时间和诱导病株生根,这进一步证实了泡桐在与植原体相互作用过程中激素代谢发生了变化.%By using Agrobacterium tumefac iens isolated from poplar crown gall disease with the hormone-produ cing genes in the T-DNA to inoculate healthy and infected Paulownia plantlets with phytoplasma, it is showed that tumorigensis of dise ased plantlets dropped apparently and the symptoms of witches' broom suppressed to some extent. The T-DNA was transformed into Paulownia resulting in tumor formation independent of exogenous hormone addition and ke eping subculture of tumor tissues for more than 2 years, thus confirming that th e tumor tissues gained the ability to synthesize cytokinin and auxin by itself. Based on the conserved sequence of isopentenyl adenosine transferase gene (ipt) of Agrobacterium tumefaciens Op ine pTil 5955 strain, a pair of DNA

  7. Volcanic Plume Elevation Model Derived From Landsat 8: examples on Holuhraun (Iceland) and Mount Etna (Italy)

    Science.gov (United States)

    de Michele, Marcello; Raucoules, Daniel; Arason, Þórður; Spinetti, Claudia; Corradini, Stefano; Merucci, Luca

    2016-04-01

    . We focus on the Holuhraun 2014 fissure eruption (Iceland) and on Mount Etna (Italy) 2013 episode. We validate our measurements against ground based measurements. The method has potential for detailed high resolution routine measurements of volcanic plume height/velocity. The method can be applied both to other multi focal plane modules push broom sensors (such as the ESA Sentinel 2) and potentially to other push-broom systems such as the CNES SPOT family and Pléiades.

  8. A case of apple moldy-core disease caused by Discostroma fuscellum in Gansu Province%甘肃省一例苹果霉心病病原鉴定

    Institute of Scientific and Technical Information of China (English)

    白滨; 文朝慧; 何苏琴

    2012-01-01

    [目的]对甘肃省天水市苹果霉心病病果中分离到的真菌进行病原鉴定.[方法]通过Koch's法则证病,采用形态学和分子生物学方法对病菌进行种类鉴定.[结果]从甘肃省天水市苹果霉心病病果中分离得到一株淡灰褐色真菌,用该菌的分生孢子悬浮液接种苹果果实,可引起与自然发病相同的苹果霉心病症状.该菌的分生孢子具有不同的形态:产生于分生孢子盘上的分生孢子具3个隔膜,极少数4-5个隔膜,纺锤形或长椭圆形,淡褐色至褐色,基细胞色稍淡,附属丝缺,孢子大小为(12.95-20.42) μm×(4.98-7.97) μm[av.(16.75±1.89) μm×(6.47±0.86) μm];产生于丝状分生孢子梗及分生孢子堆上的分生孢子具2-9个隔膜,纺锤形、棒状或蠕虫状,初始色淡,渐呈褐色,(12.45-59.76) μm×(4.98-11.21) μm [av.(30.10±11.16) μm×(7.26±l.28) μm].[结论]经形态学和分子生物学鉴定(GenBank登录号JF320818),将该病菌鉴定为Discostroma fuscellum (Berk.&Broome)Huhndorf.这是Discostroma fuscellum引起苹果霉心病在我国的首次报道.%[Objective] Identification of the pathogen of moldy-core disease of apple in Gansu Province.[Methods] The pathogenicity of fungal isolate was confirmed by Koch's rule, and identification of the pathogen was performed according to anamorphic morphological characteristics and ITS rDNA sequence analysis result.[Results] A fungus isolated from moldy-core disease of apple in Tianshui County, Gansu Province, in January 2009.The fungus caused the same symptom compared to the natural mouldy-core disease while injected inoculation through the calyx tube of apple fruits.The fungus produced conidia with different morphological types: Conidia formed on acervuli (black conidiomata developed on mycelial mat in potato sucrose liquid medium after cultured 7 months at 3 ℃-7 ℃), (12.95 -20.42) μm× (4.98-7.97)μm [av.(16.75±1.89) um×(6.47±0.86) μm], 3 septa, rarely 4-5 septa, fusiform or

  9. Phase 1 Development Report for the SESSA Toolkit.

    Energy Technology Data Exchange (ETDEWEB)

    Knowlton, Robert G.; Melton, Brad J; Anderson, Robert J.

    2014-09-01

    The Site Exploitation System for Situational Awareness ( SESSA ) tool kit , developed by Sandia National Laboratories (SNL) , is a comprehensive de cision support system for crime scene data acquisition and Sensitive Site Exploitation (SSE). SESSA is an outgrowth of another SNL developed decision support system , the Building R estoration Operations Optimization Model (BROOM), a hardware/software solution for data acquisition, data management, and data analysis. SESSA was designed to meet forensic crime scene needs as defined by the DoD's Military Criminal Investigation Organiza tion (MCIO) . SESSA is a very comprehensive toolki t with a considerable amount of database information managed through a Microsoft SQL (Structured Query Language) database engine, a Geographical Information System (GIS) engine that provides comprehensive m apping capabilities, as well as a an intuitive Graphical User Interface (GUI) . An electronic sketch pad module is included. The system also has the ability to efficiently generate necessary forms for forensic crime scene investigations (e.g., evidence submittal, laboratory requests, and scene notes). SESSA allows the user to capture photos on site, and can read and generate ba rcode labels that limit transcription errors. SESSA runs on PC computers running Windows 7, but is optimized for touch - screen tablet computers running Windows for ease of use at crime scenes and on SSE deployments. A prototype system for 3 - dimensional (3 D) mapping and measur e ments was also developed to complement the SESSA software. The mapping system employs a visual/ depth sensor that captures data to create 3D visualizations of an interior space and to make distance measurements with centimeter - level a ccuracy. Output of this 3D Model Builder module provides a virtual 3D %22walk - through%22 of a crime scene. The 3D mapping system is much less expensive and easier to use than competitive systems. This document covers the basic installation and

  10. Coaxial three-mirror-anastigmat system of high-resolution space camera%高分辨率空间相机共轴三反光学系统实现形式研究

    Institute of Scientific and Technical Information of China (English)

    李旭阳; 李英才; 马臻; 易红伟

    2009-01-01

    为了设计出高分辨率空间相机的光学系统,研究了反射式光学系统中存在次镜对主镜的中心遮拦等问题.通过对共轴三反射光学系统结构参数和最终视场在像面上的分布情况的分析,提出了半视场、全视场和环形视场3种光学形式,设计了焦距为3000mm、F数为10的3种形式的共轴三反光学系统.设计结果表明:3种共轴三反光学系统在有效视场角内成像质量均接近衍射极限,可以满足高分辨率空间相机不同的使用要求,指出半视场共轴三反光学系统适合于线阵CCD推扫成像,全视场共轴三反光学系统可用于面阵CCD凝视成像,环形视场共轴三反光学系统可实现一台相机上全色和多光谱成像的要求.%The problem that the primary mirror center is obscured by secondary mirror, existing in the reflective optical system, is researched for designing an optical system of the high-resolution space camera. Three optical systems of half, full and annular FOVs were proposed based on the analysis of the structural parameters of the coaxial three-mirror-anastigmat system and the distribution of the final FOV on the image plane. The coaxial three-mirror-anastigmat systems of three modes, whose focal length is 3 000 mm and F number is 10, are designed. The result indicates that the image quality of the three optical systems approaches the diffraction limit in the effective field, and can meet the different demand of high-resolution space camera. It is pointed out that the coaxial three-mirror-anastigmat system with half FOV is suitable to the push-broom imaging of linear array CCD, the coaxial three-mirror-anastigmat system with full FOV can be used to the staring imaging of planar array CCD, and the coaxial three-mirror-anastigmat system with annular FOV can realize the full color and multispectral imaging on a same camera.

  11. Calogênese do híbrido Theobroma grandiflorum x T. obovatum (Sterculiaceae Calogenesis of Theobroma grandiflorum x. T. obovatum hibrid (Sterculiaceae

    Directory of Open Access Journals (Sweden)

    Giorgini Augusto Venturieri

    2004-12-01

    Full Text Available O declínio da cacauicultura na amazônia tem sido causado pela doença vassoura-de-bruxa (Crinipellis perniciosa. Um híbrido interespecífico do mesmo gênero (Theobroma grandiflorum x T. obovatum tem demonstrando resistência a esta doença. Avaliou-se a calogênese deste híbrido com a finalidade de sua propagação massiva. Os explantes usados (tegumento, cotilédone e embrião foram postos para desenvolver em condições estéreis em meio de cultura LPm mais água de coco (50 ml/l, glucose (20 g/l, 2,4 - D (2 mg/l, Thidiazuron - TDZ nas concentrações de 0, 5 e 10 µg/l, carvão ativado (2 g/l em gelrite (2 g/l a um pH de 5,8. Cada concentração de TDZ x tipo de tecido foi considerado um tratamento. As culturas foram mantidas no escuro, à temperatura de 22 ± 2 ºC. Os tecidos não mostraram diferenças significativas quanto a proporção do explante recoberto por calos (p= 0,14 embora notou-se que nos cotilédones tenha sido maior. A dosagem de TDZ nitidamente não influenciou no recobrimento de calos (p= 0,94. Quanto a freqüência, os tecidos mostraram diferentes capacidades de desenvolver calos (p= 0,02 sem mostrar-se influenciados pela dosagem de TDZ (p = 0,66. Os explantes de cotilédones foram os que formaram calos em maior freqüência. Houve uma leve tendência de que quanto menor a dosagem de TDZ maior a freqüência de calos obtidos. Não foi constatada interação entre tipo de calo/frasco x dosagem de TDZ (p = 0,32.Decline of cocoa plantations in Amazonia has been attributed to Witches-broom-disease (Crinipellis perniciosa. An interspecific hybrid (Theobroma grandiflorum x T. obovatum has demonstrated resistance to this disease. Calogenesis of this hybrid was evaluated, in preparation for its mass propagation. The explants used (tegument, cotyledons and embryos were developed in sterile conditions in LPm media plus coconut milk (50 ml/l, glucose (20 g/l, 2,4 - D (2 mg/l, Thidiazuron - TDZ at concentrations of 0, 5 e 10 µg

  12. 藜麦新品种陇藜1号的选育及应用前景%Breeding and Application Prospects of New Variety Chenopodium quinoa cv. Longli 1

    Institute of Scientific and Technical Information of China (English)

    杨发荣

    2015-01-01

    陇藜1号是甘肃省农业科学院畜草与绿色农业研究所采用系统育种和栽培驯化相结合的方法选育而成的国内首个藜麦新品种。在2013—2014年进行的多点区域试验中,陇藜1号2 a 10点(次)平均折合产量为2100.0 kg/hm2,比对照品种静乐藜麦增产9.6%。陇藜1号属中晚熟品种,植株呈扫帚状,株高181.2~223.6 cm,生育期128~140 d,分枝数23~27个。种子为圆形药片状,千粒重2.40~3.46 g。籽粒含粗蛋白171.5~187.8 g/kg、脂肪56.5~59.3 g/kg、赖氨酸5.5~6.9 g/kg、全磷4.5~6.8 g/kg。陇藜1号在田间表现为抗霜霉病和叶斑病,总体抗病能力强。陇藜1号植株抗倒伏,再生能力强。具有耐寒、耐旱、耐盐碱、耐瘠薄等特性,适应性广。适宜在甘肃省无霜期大于120 d,降水量250 mm以上,海拔1500~3000 m的山地、川地及灌溉区域种植。并对藜麦的应用前景进行了展望。%Chenopodium quinoa cv. Longli 1 is the first new variety in China,which is bred by pasture and green Agriculture Institute of Gansu Academy of Agricultural Sciences through the methods of line breeding,cultivation and domestication. In multiple locations of regional tests,the average yields of Longli 1 are 2 100.00 kg/hm2,which is increased 9.6%compared with control. Longli 1 is mid-late maturing quinoa variety. The plant likes brooms,which plant height is 181.2~223.6 cm,growth period is 128~140 d,and the numbers of branch are 23~27. The seeds are round pill shaped,the weight of a thousand seeds is 2.40~3.46 g. Crude protein,fat, proline,total phosphorus contents in seeds are 171.5~187.8 g/kg,56.5~59.3 g/kg,5.5%~6.9 g/kg and 4.5~6.8 g/kg,respectively. Longli 1 displays the strong disease-resistant ability. Longli 1 shows lodging resistance and strong regeneration. It has some physiological characteristics,such as cold-resistant,drought-resistant and salty-resistant. It is suitable for planting in hilly and irrigation areas in

  13. Characteristics of bryophytes with gemmae from a karst bauxite mine:A case study of Lindai mine in Zhanj ie town,Qingzhen,Guizhou%贵州喀斯特铝土矿具芽胞苔藓种类特征--以贵州省清镇市林歹铝土矿区为例

    Institute of Scientific and Technical Information of China (English)

    殷声锋; 王智慧; 张朝晖

    2014-01-01

    .appeared in four different habitats.(4)In summary,bryophytes with gemmae preferred to the rela-tive pollution conditions.(5)The gemmae of Bryumbornholmense Winkelm.&Ruthe.and Bryumapicula-tum Schwagr.occurred on the rhizoids,while other kinds of gemmae were found on the axil which were in the shape of broom.The forms of gemmae were multiple such as sphere,clavate,wooden stick,bud,spin-dle and torch.

  14. Preliminary hyperspectral volcano observations using Airborne Radiative Spectral Scanner (ARTS)

    Science.gov (United States)

    Jitsufuchi, T.

    2008-12-01

    Airborne-imaging spectral systems can often efficiently identify volcanic phenomena that are difficult to detect by satellite imagery. Since 1990, the National Research Institute for Earth Science and Disaster Prevention (NIED) has been developing our original airborne-imaging spectral systems for volcano observations. In 2006, we developed a new airborne hyperspectral sensor, the Airborne Radiative Transfer Spectral Scanner (ARTS), for hyperspectral volcano observations. ARTS is a push-broom imaging spectrometer covering wavelengths from 380 to 1100nm (VNIR; 288 bands), 950 to 2450nm (SWIR; 101 bands), and 8000 to 11500nm (LWIR; 32 bands) and has precise position and attitude measurement systems (GPS/IMU) to achieve direct geo-correction of the acquired image. The ARTS specifications were planned to provide hyperspectral images to support developing algorithms for remotely sensing the geothermal distribution, ash- fall areas, and content of volcanic gas columns. ARTS will also be useful for operational volcanic observations to assess volcanic activity and to mitigate volcanic disasters.Before beginning the operational use of ARTS, it is important to validate its in-flight performance. Therefore, we have been conducting validation on the B200 platform. In this study, we present the results of two experiment observations, the overflight of ARTS instrument at the NIED building site on April 5, 2007, and the volcano observations flight over active volcano (Sakurajima volcano) just after its eruption on April 8, 2008. At the NIED building site, we validated the radiometric fidelity of all bands and the accuracy of geo-corrections. At the Sakurajima volcano, we tried to demonstrate the functions of ARTS, especially those for volcano observation. At the NIED building site, the validation results indicate that the geo-correction accuracy is typically less than a two-pixel difference (RMS), and that there was good agreement between the predicted radiance at the sensor and

  15. 马尔尼菲青霉菌培养与鉴定%Culturing and Identification of Penicillium marneffei

    Institute of Scientific and Technical Information of China (English)

    唐秀文; 刘存旭; 李月水; 杨晓辉

    2009-01-01

    OBJECTIVE To improve the diagnosis level of Penicillium marneffei culture identification in the grass-roots laboratories. METHODS The patient's blood or bone marrow were innoculated to BacT/Alert 3D blood culture system, when positive sample was found, the culture fluid and node tissues were smeared to take, Gram stain. The pathogens suchas small long branch, septate hyphae, sausage-shaped cell, were transferred to Colombia blood plate, sand Paul agar and CHROMagar Candida chromogenic culture medium to observe the 25 ℃ and 35 ℃, to observe the colony morphology and cell characteristics. The relevant biochemical reaction tests, were cutler taken. RESULTS The foundation of identifying P. marneffei culture: it is a dimorphic fungus, cultured for fungal phase at 25℃ ,and for yeast phase at 35℃. Both could produce wine red pigment. Morphology under the microscope.culture had typical broom-shaped sticks at 25℃,and round, oval, blunt and slightly curved at both ends,and sausage-shaped thallus that had horizontal septum. Biochemical results=both could assimilate and ferment glucose to produce acid, urea luciferase positive. Both could be inhibited by Actidione, and couldn't assimilate fermenting lactose. CONCLUSIONS The appropriate medium for the P. marneffei is Sabourand's medium. The appropriate stains of the cell morphology and structure are lactic acid cotton blue staining and Gram staining which are suitable for the grass-root level laboratories.%目的 提高马尔尼菲青霉菌(PMA)培养鉴定在基层实验室的诊断水平.方法 取患者血液或骨髓液置于BacT/Alert 3D血培养仪系统配套的成人中和抗生素培养瓶,经培养提示阳性,取阳性培养物和淋巴结组织涂片、革兰染色镜检见病原体有细长分枝、分隔菌丝、腊肠形菌体,转种到哥伦比亚血平板、萨布罗琼脂及CHROMagar假丝酵母菌显色培养基,观察25℃和35℃孵育时的菌落形态及镜下菌体特征;测试相关生

  16. [Anatomy and uses of the mature leaves of three species of Sabal (Arecaceae) of the Yucatan, México].

    Science.gov (United States)

    Pérez, Martha; Rebollar, Silvia

    2003-06-01

    This paper describes the leaf anatomy of Sabal mauritiiformis (Karst.) Griseb. & H. Wendl., Sabal mexicana Mart. and Sabal yapa Wright ex Becc., three of the four most representative species of the Yucatán Península, in Mexico. These species are locally used: in the roofing of traditional homes, as food (fruits and apical buds), and in the production of hats, brooms and handicrafts. Leaf samples were collected in secondary growth of lower montane rainforest in the state of Quintana Roo and in two home gardens in the state of Yucatán. Herbarium samples were obtained, and samples of blade and petiole were fixed in formaline-acetic acid-alcohol. Cross incisions were made on the blade and petiole, and were dyed with safranin and toluidine blue O. The results show that S. mauritiiformis and S. yapa are morphologically alike: both are tall, slim palm trees; the leaf in S. mauritiiformis is a shorter palm-like structure compared with the other two species. The shape of the main nerve, as seen in cross section, is rectangular in the three species. The hastula in the three species is acuminate and adaxial. The foliar anatomic structure is similar in the three species, although there are some differences. The adaxial an abaxial epidermis of the blade consist of one layer and, superficially, the anticlinal walls are straight; the stomata are intercostal, of the tetracytic type, present on both surfaces in S. mexicana and S. yapa and only on the abaxial surface on S. mauritiiformis. The hypodermis is one layer thick in S. yapa and in S. mexicana and two layers thick in S. mauritiiformis. In the three species the palisade parenchyma consists of several undefined strata as the cells are similar-in shape and size--to the cells in the spongy parenchyma, so there is no marked difference between these strata and the spongy parenchyma seems almost continuous. Both fibrous and vascular bundles are distributed between the hypodermis and the palisade parenchyma; the fiber bundles can

  17. Levantamento etnobotânico de plantas medicinais na cidade de Ipameri - GO Ethnobotanical survey of medicinal plants in Ipameri City - Goiás State

    Directory of Open Access Journals (Sweden)

    M.R. Zucchi

    2013-01-01

    of these plants; and to diagnose the gender profile and the age and wage ranges of users. Thus, structured interviews were conducted with 200 families in the city and plants were collected for their correct identification. The material was herborized, identified and deposited in the Herbarium of "Universidade Estadual de Goiás" (HUEG. Of the 200 families interviewed, 75 said they did not make use of plants for medicinal purposes (37.5%, while 125 said they do use them (62.5%. The latter group reported the 35 most used species: "hortelã-rasteira" (Mentha x villosa L., "boldo-sete-dores" (Plectranthus barbatus Andrews., lemon grass (Cymbopogon citratus (DC. Stapf., "quebra-pedra" (Phyllanthus niruri L., chamomile (Chamomilla recutita (L. Rauschert., pennyroyal (Mentha pulegium L., guaco (Mikania glomerata Spreng., mentrasto (Ageratum conyzoides L., clove basil (Ocimum gratissimum L., wormwood (Artemisia canphorata Vill., balm (Eysenhardtia platycarpa Mich., broom (Baccharis trimera (Less. DC., fennel (Foeniculum vulgare Mill., aloe (Aloe vera L. and mallow (Althaea officinalis L.. All consumer families (100% stated to prefer plants grown organically, to select the plants based on their good appearance (68% families and to eat them in natura (unprocessed, 100%. The use of medicinal plants in Ipameri is independent of gender (54% womem and 46% men and extends to several age and socioeconomic ranges, configuring thus a good consumer market.

  18. Estimation of biological nitrogen fixation by black locust in short-rotation forests using natural 15N abundance method

    Science.gov (United States)

    Veste, M.; Böhm, C.; Quinckenstein, A.; Freese, D.

    2012-04-01

    .4% (C/N 14.4) in 3 year-old trees, respectively. A higher content of nitrogen was found in leaves of re-sprouted trees with 4.3% (C/N 11.5). The estimated percentage of nitrogen derived from the atmosphere (% NdfA) in black locust was 63% - 83% compared to 56% in seabuckthorn (Hippophaë rhamnoides) and 79% in common broom (Genista scuparia). The annual leaf biomass production of black locust varied between 1325 (2 years old trees) and 2576 kg/ha a (4 years old trees). The estimated leaf nitrogen fixed by Robinia was approx. 30.5 - 59.2 kg/ha a. From the results, we can conclude that the biological nitrogen fixation by Robina is an important factor for the nitrogen balance of short-rotation plantations on nutrient poor-soils.

  19. Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae Antimicrobial activity of essential oils produced by Baccharis dracunculifolia D.C. and Baccharis uncinella D.C. (Asteraceae

    Directory of Open Access Journals (Sweden)

    Regina Ferronatto

    2007-06-01

    , antioxidant, antimicrobial, cytotoxic and antiinflammatory effects. The broom is a bushy plant which occurs spontaneously in Brazil as well as in the other countries from Mercosul. The oil composition depends on the geographical region and on the concentration of oxygenated blends, highlighting the nerolidol and the sphatulenol. Essential oils obtained from these two species by the hydrodistillation process were assessed by the diffusion method in paper disc, in Petri plaques containing Müeller-Hinton agar, with bacteria suspensions, previously adjusted to the tube 0,5 of Mac Farland scale of four strains which came from the American Type Culture Collection: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Discs containing different volumes of the essential oils (1, 3, 5 and 10 (µL/disc were added to plaques and incubated at 36 ºC for 24 - 48 hours. Chloranfenicol (CLO and Amoxicillin (AMO with concentrations of 30 and 10 µg/disc were respectively used as control. The presented results reveal that both assessed oils present antimicrobial activity against S. aureus, E. coli and P. aeruginosa.

  20. El fuego favorece la invasión de Teline monspessulana (Fabaceae al aumentar su germinación Fire promotes Teline monspessulana (Fabaceae invasion by increasing its germination

    Directory of Open Access Journals (Sweden)

    RAFAEL A GARCÍA

    2010-01-01

    fires on the seed bank of Teline monspessulana (L. K. Koch (French Broom and its implications for the invasión of the species in the mediterranean area of south-central Chile. For this aim, soil seedbank samples were subjected to different treatments that simúlate fire conditions through controlled burns. In addition, for seeds taken from mature plants the optimal range of temperatures at which germination occurs was determined. The results show that after a wildfire germination of T. monspessulana increases significantly. Heating of the seeds was the factor that triggers this increase in germination. Furthermore, it was determined that the seeds reach their maximum germination rate when preheated between 80 and 120 °C for 10 minutes. At 140 °C, 100 % of the seeds die. During a fire, optimal temperatures for germination are reached about 2 cm in depth within the soil or at the surface in areas where the accumulation of fuel (biomass does not exceed 5 tons ha4. The small portion of the seed bank that manages to be stimulated is offset by the large seedbank that can exceed 8000 seeds m"². This study shows that the fire, a common phenomenon in mediterranean areas and increasingly frequent in central Chile, would be promoting the persistence of T. monspessulana in areas with high human disturbance.

  1. Estructura básica del baile flamenco. Basic structure of flamenco dancing.

    Directory of Open Access Journals (Sweden)

    Mónica González Sánchez

    2011-05-01

    Full Text Available Pautas estructurales aplicables a la hora de coreografiar un baile tradicional flamenco. Las partes principales de las que consta la coreografía de un palo flamenco tradicional son: 1ª Letra; 2ª Letra; Escobilla; y Final. Podemos decir que estas serían partes ineludibles de un palo. En este artículo señalamos además, qué opciones tiene cada elemento que conforma el hecho flamenco (cante-baile-guitarra, esto es, qué puede hacer en cada una de esas partes. Así se proporciona al lector herramientas funcionales a la hora de crear una coreografía flamenca como coreógrafo o intérprete, o bien a la hora de analizarla, como espectador.Se explican las estructuras de algunos palos que por sus particularidades, se diferencian del resto. Uno de ellos es el baile por alegrías, donde señalamos aquellas partes que lo convierten en uno de los palos estructuralmente más definidos. Se hace hincapié en el silencio de alegrías, parte que lo diferencia del resto de cantiñas o cantes de Cádiz. También se propone una estructura para el baile por caña y polo, cuyos cantes tienen una sucesión de ayes característicos que se repiten varias veces en su interpretación. Finalmente se muestra una posible estructura para tangos y bulerías, pero como palos propios no como bailes festeros o final de otros palos.Structural guidelines which are to be applicable at the time to choreograph a traditional flamenco dance.The main parts which the choreography of each traditional variety (or palo of flamenco dance consists of are: 1st lyric, 2nd lyric, broom (or escobilla, and Final. We can say that these should be considered the basic elements all varieties must include. In this article we also point out what role each element that makes up the flamenco (song-dance-guitar plays; that is, what each one of those elements can do. In this way, we provide the reader with functional tools when making flamenco dance, both as a choreographer and a performer; and even

  2. A Radiation Hard Multi-Channel Digitizer ASIC for Operation in the Harsh Jovian Environment

    Science.gov (United States)

    Aslam, Shahid; Aslam, S.; Akturk, A.; Quilligan, G.

    2011-01-01

    ultimately impact the surface of Europa after the mission is completed. The current JEO mission concept includes a range of instruments on the payload, to monitor dynamic phenomena (such as Io's volcanoes and Jupiters atmosphere), map the Jovian magnetosphere and its interactions with the Galilean satellites, and characterize water oceans beneath the ice shells of Europa and Ganymede. The payload includes a low mass (3.7 Kg) and low power (< 5 W) Thermal Instrument (TI) concept for measuring possible warm thermal anomalies on Europa s cold surface caused by recent (< 10,000 years) eruptive activity. Regions of anomalously high heat flow will be identified by thermal mapping using a nadir pointing, push-broom filter radiometer that provides far-IR imagery in two broad band spectral wavelength regions, 8-20 m and 20-100 m, for surface temperature measurements with better than a 2 K accuracy and a spatial resolution of 250 m/pixel obtained from a 100 Km orbit. The temperature accuracy permits a search for elevated temperatures when combined with albedo information. The spatial resolution is sufficient to resolve Europa's larger cracks and ridge axial valleys. In order to accomplish the thermal mapping, the TI uses sensitive thermopile arrays that are readout by a custom designed low-noise Multi-Channel Digitizer (MCD) ASIC that resides very close to the thermopile linear array outputs. Both the thermopile array and the MCD ASIC will need to show full functionality within the harsh Jovian radiation environment, operating at cryogenic temperatures, typically 150 K to 170 K. In the following, a radiation mitigation strategy together with a low risk Radiation-Hardened-By-Design (RHBD) methodology using commercial foundry processes is given for the design and manufacture of a MCD ASIC that will meet this challenge.

  3. COVER CROPS EFFECTS ON THE DEVELOPMENT OF UPLAND RICE UNDER ORGANIC FARMING SYSTEM PLANTAS DE COBERTURA DE SOLO E SEUS EFEITOS NO DESENVOLVIMENTO DA CULTURA DO ARROZ DE TERRAS ALTAS EM CULTIVO ORGÂNICO

    Directory of Open Access Journals (Sweden)

    Roberta Paula de Jesus

    2007-12-01

    Full Text Available

    This research was conducted in an experimental area in Santo Antônio de Goiás (16º28'S, 49º17'W and altitude 823 m, Brazil, during the months of June, 2004, and March, 2005. The upland rice variety Aimoré was used along with several cover crops aiming to evaluate leaf area, number of tillers, dry matter, and nitrogen content in the phytomass during the rice crop cycle. The experimental design was the randomized blocks one, with five treatments and four replications. The treatments consisted of different cover crops, such as velvet bean (Mucuna aterrima, sunn hemp (Crotalaria juncea, dwarf pigeon pea (Cajanus cajan, broom sorghum (Sorghun bicolor, and a check treatment with spontaneous vegetation growing among the rice plants. The leguminous plants, specially C. juncea, presented better results in tillering production, leaf area index, dry matter yield, and accumulated nitrogen content, if compared to the treatments where grasses were used as soil cover crop. It was concluded that rice presented a satisfactory development in the different soil cover treatments, specially after leguminous cultivation.

    KEY-WORDS: Rice, green manure, leaf area index, dry matter.

    O experimento foi conduzido numa área experimental em Santo Antônio de Goiás (16º28'S, 49º17'W e altitude de 823 m, no período de junho de 2004 a março de 2005. Utilizou-se a cultivar Aimoré de arroz de terras altas, em seqüência a diferentes plantas de cobertura de solo, com o objetivo de avaliar o índice de área foliar (IAF, número de afilhos, acúmulo de massa de matéria seca (MMS e o teor de nitrogênio acumulado na fitomassa durante o ciclo da cultura do arroz. O delineamento experimental foi o de blocos ao acaso, com cinco

  4. 一株拮抗香蕉枯萎病的青霉菌的分离及鉴定%Isolation and Identification of a Penicillium Strain against Banana Wilt Disease Caused by Fusarium Oxysporum f.sp.Cubense

    Institute of Scientific and Technical Information of China (English)

    王宇光; 卢娟; 孙建波; 夏启玉; 卢雪华; 张欣

    2011-01-01

    旨在从海南土壤中分离对香蕉枯萎病病原菌4号小种(FOC4)有较强拮抗作用的微生物.采用对峙实验法从海南土壤中分离到一株对FOC4有较强拮抗作用的菌株QW1,对该菌株进行了显微特征、菌落特征以及26S rDNA序列分析.此外,将该菌株与FOC4在土壤中进行了共培养实验研究.结果表明,该菌株在土豆葡萄糖培养基上生长的内生菌丝无横隔膜;孢子梗顶端膨大成帚状;单个分生孢子为椭圆形,蓝色;产有性孢子.菌落生长开始为白色,培养成熟后,气丝逐渐因产生孢子由白色变蓝色.以26S rDNA序列为基础构建了相关种属在内的系统发育树,其与青霉菌株的26S rDNA序列的同源性大于为99%.将该菌株鉴定为青霉菌.共培养实验表明,该菌株在土壤中有较强的生长力,且能有效抑制病原菌的生长.青霉菌QW1对FOC4有较强的拮抗作用,并且与FOCA在土壤中共培养的实验中也表现出较强的抑制FOCA的生长作用.%In order to get some antagonistic microorganism against banana wilt disease caused by fusarium oxysporum f. sp. cubense race 4 (FOC4) from soil in Hainan province. Antagonistic microorganism QW1 was isolated by flat confrontation culture from soil in Hainan province. The morphological characteristics, colony characteristics and 26S rDNA sequences analysis of QW1 were studied. Moreover, QW1 and FOC4 were co-cultured in soil for one month. The results showed that: QW1 produced sexual spores. The substrate myceliums had no partition. The tops of conidiophores swelled to broom shape. Single-conidiospore was oval and blue. The colony of QW1 was white when it starts growing on PDA medium, after matured; the aerial myceliums of QW1 became blue gradually because of spores. A phylogenetic tree was constructed by comparing with the published 26S rDNA sequences of the related species. The QW1 strain showed more than 99% identity with Penicillium. QW1 strain was classed to Penicillium

  5. Hydraulic characterization of " Furcraea andina

    Science.gov (United States)

    Rivera-Velasquez, M. F.; Fallico, C.; Molinari, A.; Santillan, P.; Salazar, M.

    2012-04-01

    constant head we repeated the test for three different rate of fiber compaction within the flow cell, corresponding to three different densities (146 kg/m3, 200 kg/m3 and 240 kg/m3). We observed that with increasing density of the fibers there is an increase of hydraulic conductivity. The importance of the experimental results on permeability acquires greater relevance when compared to the values of hydraulic conductivity already known from the literature for different soils types. In this study, special attention has been given to the value of hydraulic conductivity of this fiber, considering that in the framework of groundwater remediation strategies if the hydraulic conductivity in the barrier is lower than that of the surrounding aquifer, the plume may change its natural pathway, bypassing the barrier and veering towards larger hydraulic conductivity. Reference • Lee, B. G. and Rowell, R. M.: Removal of heavy metal ions from aqueous solutions using lignocellulosic fibers, J. Natural Fibers, 1, 97-108, 2004. • Troisi, S., Fallico, C., Straface, S., and Mazzuca, L.: Biodreni per la bonifica di siti contaminati realizzati con fibre naturali liberiane ad elevato sviluppo superficiale. Brevetto dell'Università della Calabria (n.: CS2008A00018), 2008. • C. Fallico, S. Troisi, A. Molinari, and M. F. Rivera.: Characterization of broom fibers for PRB in the remediation of aquifers contaminated by heavy metals, Biogeosciences journal , 7, 2545-2556, 2010

  6. Seleção de descritores botânico-agronômicos para caracterização de germoplasma de cupuaçuzeiro Selection of morpho-agronomic descriptors for cupuaçuzeiro germplasm characterization

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2003-07-01

    the final selection was done. Three criteria for variable exclusion were adopted. Thirty four descriptors were excluded, representing a reduction of 64%. A minimal list of descriptors for cupuaçuzeiro was proposed, including leaf length, leaf thickness, leaf apex width, leaf base vein angle, flower bud length, flower peduncle length, flower peduncle diameter, ovary girth, number of ovules, flower petal lamina length, staminode length, seed width, flat seeds, acidity, brix, pH, number of fallen flowers buds, number of abscised immature pods and number of witches-brooms.

  7. Some aspects of chicken behavior and welfare

    Directory of Open Access Journals (Sweden)

    LS Costa

    2012-09-01

    with the European Union's (EU guidelines for animal protein production, Brazilian poultry production needs to undergo a process of adaptation. In May, 2007, the EU Commission established its new guidelines for animal welfare in poultry production, pressured by consumer demand. In the EU, there is a growing concern among consumers as to how poultry are reared and slaughtered. European consumers are in the fore front of the demand of high quality products produced with under better welfare conditions, and have spread this concern throughout the world. Beaumont et al. (2010 mentioned that European consumers frequently perceive that standard commercial poultry production has poor animal welfare practices. According to Nääs et al. (2008, Brazilian poultry production today needs to find a balance between cruelty and productivity. In fact, ensuring animal welfare may provide better financial results, as it increases the producer's profit margins and allows maintaining Brazilian chicken export quotas to the EU. França (2008 noted that biological studies that define ethical limits and guidelines for poultry production foster the development of new production practices that may ensure good product quality and productivity without putting bird welfare at risk. Gonyou (1994 states that, when animal welfare started to be studied, the only behavioral factors considered were those related to feeding and reproduction. These first studies used as indicators of animal welfare reduced life expectancy, impaired growth, impaired reproduction, body damage, disease, immunosuppression, adrenal activity, behavior anomalies, and self-narcotization (Broom, 1991. However, current studies evaluate additional indicators, such as natural behaviors, behavioral needs, preferences, behavioral problems, emotional state, cognitive abilities, etc. In the field of ethology, the expression of natural behavior is a frequently used tool used to estimate the welfare of poultry destined to human consumption

  8. [Man and his fellow-creatures under ethical aspects

    Science.gov (United States)

    Teutsch

    1999-01-01

    Preliminary remarks Preceding the detailed literary review, here a few events, topics and publications for the busy reader including * The decision of the Bundesverfassungsgericht (Federal Constitutional Court) to declare unconstitutional and invalid chicken owner ordinances permitting caging * The decision of the German parliament to declare animal protection a national goal * Publication of a number of books which are likely to influence discussions for years to come since they bring to a close developments having emerged over an extended period of time. Without claiming to be exhaustive the following should be mentioned: Marc Bekoff and Carron A. Meany, ed.: "Encyclopedia of animal rights and animal welfare", an extensive work being reviewed by Peter Thornton in chapter 3.6 of this report. For the first time, animal protection of the Anglo-American tradition is being summarised, in theory as well as in practice, and, on high standards: "The list of contributors reads like a Who"s Who of experts in their chosen fields and includes philosophers such as Peter Singer, Tom Regan, Tom Beauchamp and Bernard Rollin and welfare scientists such as Don Broom, David Fraser, Temple Grandin. Others involved in examining the role of animals in society and our relationship with them, such as Andrew Linzey, Richard Ryder, James Serpell and David Morton (to name just a few), have also provided entries" (Peter Thornton). The comprehensive monograph by Johannes Caspar: "Tierschutz im Recht der modernen Industriegesellschaft. Eine rechtliche Neukonstruktion auf philosophischer und historischer Grundlage" (Animal Protection in the Law of modern industrial Society. A new legal Construct on a philosophical and historical Base) represents an extensive critical incorporation of animal protection in Germany under legal and ethical aspects including a detailed rendering of the actual treatment of animals. The term "critical incorporation" was used on purpose since never before was cruelty

  9. Gorduras de descarte e componentes externos do corpo de novilhos e vacas de descarte de diferentes grupos genéticos Trim fat and external components of the body of steers and cull cows from different genetic groups

    Directory of Open Access Journals (Sweden)

    Jonatan Cattelan

    2010-12-01

    cows, respectively. The animals were maintained at feedlot finished until they reached the finishing degree for slaughter. The experimental diet had a roughage concentrate ratio of 40:60 (dry matter basis, containing 14.2% of crude protein and 2,869kcal of digestible energy kg-1 dry matter. The experimental design was complete randomized in a 2x2 (two categories and two genetic groups factorial scheme. The slaughter weight (PAB (509.67 versus 414.50kg and the empty body weight (PCVZ (433.01 versus 355.27kg, respectively were higher for cows in relation to steers. When the components were expreseds in absolutes values and adjusted for PCVZ and PAB, cull cows showed higher inguinal fat, renal fat and internal total fat weights. When the components weights were adjusted for PAB e PCVZ, the steers showed higher head, foots, tail broom and total external components. The 5/8Ch 3/8Ne animals showed higher head absolute weight and ruminal fat in relation to PCVZ, while the 5/8Ne 3/8Ch showed higher rawhide weight in relation to PAB and PCVZ, and in relation to PAB higher total extern components weight. The carcass dressing percentage and non integrants body parts of carcass didn't show any correlation.

  10. The effect of the fires on gypseous soil properties: changes of the hydrology and splash resistance.

    Science.gov (United States)

    León, J.; Seeger, M.; Echeverría, M.; Badía, D.; Peters, P.

    2012-04-01

    Mediterranean ecosystems have been severely affected by fires in the last decades. Due to social and economical changes, wildfires have caused hydrological and geomorphologic changes to be more pronounced, resulting in enhanced soil erosion. Soil heating caused by fires affects soil aggregates stability, water infiltration and may generate hydrophobicity. In order to understand how wildfire affects soil hydrological behavior in general, and splash and runoff processes in particular, of gypsum soils,it is advantageous to use a rainfall simulator. In August 2009 a large forest fire affected 6700 ha in Remolinos (NW Zaragoza, Spain). The area is covered by shrubs such as gorse (Genista scorpius L.), broom (Retama sphaerocarpa L.) and rosemary (Rosmarinus officinalis L.), and with small areas occupied by Aleppo pine (Pinus halepensis Mill) and Kermes evergreen-oak (Quercus coccifera L.). This region has a semarid Mediterranean climate, with an average annual rainfall ca 560 mm and a mean annual temperature of 12.5°C, resulting in an estimated climatic water deficit of ca. 400mm. The relief consists of stepped slopes (200-748 m), on two different types of soil have developed: Renzic Phaeozem, on limestone, and Haplic Gypsisol, on gypsum (IUSS, 2006). Within this study, we wanted to investigate the differences in affection by fire of the different soil types, as it may be caused by different fire intensities. Therefore, both soil types were sampled after fire. Also, similar locations were sampled which were not affected by the wildfires. With this, we could differentiate 4 treatments: burnt and unburnt pine forest and burnt and unburnt shrub on gypseous soils. We designed a set of lab experiments to elucidate the effect of heat on soil composition, aggregate stability, and splash susceptibility. Samples were taken using cylinders of 5 cm depth. Under laboratory conditions were measured pH, CE, organic matter (OM), soil aggregates stability (SAS), bulk density, porosity

  11. Sedimentary sequence and paleogeographic evolution of Albian Stage,Lower Cretaceous in Block A of Lower Congo Basin%下刚果盆地A区块下白垩统Albian阶沉积层序与古地理演化

    Institute of Scientific and Technical Information of China (English)

    于水; 文华国; 郝立华; 郝立业; 程涛; 郑荣才

    2012-01-01

    of peripheral diamictic-fractured platform and each sequence can be further divided into TST and HST systems tracts. The response characteristics of sedimentary sequences and sedimentary filling of mixed siliciclastic-carbonate rock are analyzed. The sedimentary evolution model of the gang control of multiple factors including "slippage, salt arch and conversion" is established. Besides, at the SQ3 sequence stage, the fractured and diamictic platform edge shoal with a broom-like distribution and the development of vertical accretion on the top of the salt arch structure will be the most favorable microfacies in the area. By taking TST and HST systems tracts as the two mapping units, the authors draw a lithofacies paleographic map of each system tracts, discuss the distribution of the favorable reservoir facies, and then make a prediction about Albian in Block A. Finally the authors think that the superimposed platform edge shoal facies in the SQ2-HST, SQ3-TST and SQ3-HST system tracts should be the key areas in the future exploration.

  12. 近50年西南地区极端干旱气候变化特征%Climate Characteristics of the Extreme Drought Events in Southwest China during Recent 50 Years

    Institute of Scientific and Technical Information of China (English)

    贺晋云; 张明军; 王鹏; 王圣杰; 王兴梅

    2011-01-01

    extreme drought frequency has presented a decreasing trend, and the high frequency area appeared alternately in the southeast - northwest - east of Southwest China; the 1990s is the most wettest decade and it is wet over the whole area. In the 2000s, the extreme drought frequency rises quickly, but the regional differences reduce. (2) In summer monsoon, the extreme drought frequency is growing, which generally happens in the high mountains around the Sichuan Basin, most parts of Guangxi and "the broom-shaped mountains". It is obvious that the altitude has impacts on the extreme drought frequency of summer monsoon; in winter monsoon, the area is relatively wet and the extreme drought frequency is decreasing. (3) In summer monsoon, the abrupt change happened in 2003, while the abrupt change of winter monsoon happened in 1989, and the joint abrupt changes in different periods lead to the extreme drought frequency variation. The departure sequence vibration of annual extreme drought frequency is quasi 5 years and quasi 12 years.

  13. SUSTENTABILIDADE DE SISTEMAS ORGÂNICOS COM PLANTAS DE COBERTURA NA CULTURA DO ARROZ, POR MEIO DE ALTERAÇÕES FÍSICAS DO SOLO ORGANIC SYSTEMS SUSTAINABILITY USING COVER CROPS IN RICE CULTIVATION THROUGH SOIL PHYSICAL ATTRIBUTES CHANGES

    Directory of Open Access Journals (Sweden)

    Roberta Paula de Jesus

    2010-05-01

    ísica intermediária. A porcentagem de agregados com diâmetro maior que 2 mm e o diâmetro médio ponderado dos agregados foram maiores, independentemente de cobertura de solo, na camada 0,10-0,20 m de profundidade. Também, independentemente da cobertura de solo, o conteúdo de matéria orgânica decresceu com a profundidade do solo.

    PALAVRAS-CHAVE: Plantas de cobertura; preparo do solo; porosidade; estabilidade de agregados.

    To evaluate the influence of cover crops on Oxisol physical attributes, in an organic production system with conventional tillage, a study was conducted at Embrapa Arroz e Feijão, Santo Antônio de Goiás, Goiás State, Brazil, for two years, in a succession cover crops-rice, in which cover crops were sown in the autumn/winter and the subsequent crop, rice cultivar Aimoré, was sown in the summer. Five cover crops were used: Velvet bean (Mucuna aterrima, Sunn hemp (Crotalaria juncea, Pigeon pea (Cajanus cajan, Broom sorghum (Sorghum technicum, and spontaneous vegetation fallowing. A randomized blocks design with four replications was used. In the second year of

  14. Aspectos agronômicos da cultura da mandioca (Manihot utilissima Pohl

    Directory of Open Access Journals (Sweden)

    Edgard S. Normanha

    1950-07-01

    cuttings. Data from field trials indicated that a spacing ranging between 0,80 x 0,40 cm and 1,00 x 0,60, according to soil fertility, is more advantageous than the spacing of 1,20 x 0,60 cm as it is usually adopted. Test on depth of planting were made with 15 cm long cuttings planted in furrows 5, 10 and 15 cm deep, and subsequently covered. The plants grown from cuttings planted 15 cm deep produced less and were more difficult to be dug out. Planting at 5 cm depth is also inadvisable because the plants may be easily uprooted by erosion or strong winds. Planting at 10 cm depth is to be recommended. Studies on cassava bacterial wilt caused by Xanthomonas manihoti (Arthaud-Ber-thet Burk, revealed that several common varieties and clones derived from seedlings show more resistance than the usually cultivated types. Cuttings of the resistant types were released to the growers and are now being widely used. A virus disease of the witches broom type, present in some localities, caused severe losses in cassava plantings made with the variety "Vassourinha". Tests carried out in infested areas showed that the variety "Brava da Ponte" is highly resistant to the disease, and the varieties "Preta" and "Holandi do Itaguá" show a fair degree of resistance.