Sample records for brooders

  1. Effects of Dark Brooders on Behavior and Fearfulness in Layers

    DEFF Research Database (Denmark)

    Riber, Anja Brinch; Guzman, Diego A.


    . Behavioral observations were performed during the first six weeks of age. Three different fear tests were conducted when the birds were age 3–6, 14–15 and 26–28 weeks. During the first four days, brooder chicks rested more than control chicks whereas they spent less time drinking, feather pecking...

  2. Multiple mating and clutch size in invertebrate brooders versus pregnant vertebrates. (United States)

    Avise, John C; Tatarenkov, Andrey; Liu, Jin-Xian


    We summarize the genetic literature on polygamy rates and sire numbers per clutch in invertebrate animals that brood their offspring and then compare findings with analogous data previously compiled for vertebrate species displaying viviparity or other pregnancy-like syndromes. As deduced from molecular parentage analyses of several thousand broods from more than 100 "pregnant" species, invertebrate brooders had significantly higher mean incidences of multiple mating than pregnant vertebrates, a finding generally consistent with the postulate that clutch size constrains successful mate numbers in species with extended parental care. However, we uncovered no significant correlation in invertebrates between brood size and genetically deduced rates of multiple mating by the incubating sex. Instead, in embryo-gestating animals otherwise as different as mammals and mollusks, polygamy rates and histograms of successful mates per brooder proved to be strikingly similar. Most previous studies have sought to understand why gestating parents have so many mates and such high incidences of successful multiple mating; an alternative perspective based on logistical constraints turns the issue on its head by asking why mate numbers and polygamy rates are much lower than they theoretically could be, given the parentage-resolving power of molecular markers and the huge sizes of many invertebrate broods.

  3. Variation in lipid and free fatty acid content during spawning in two temperate octocorals with different reproductive strategies: surface versus internal brooder (United States)

    Viladrich, Núria; Bramanti, Lorenzo; Tsounis, Georgios; Chocarro, Blanca; Martínez-Quitana, Angela; Ambroso, Stefano; Madurell, Teresa; Rossi, Sergio


    This study investigates the energetic investment during spawning of two Mediterranean gorgonians characterized by different reproductive strategies: Corallium rubrum (internal brooder) and Paramuricea clavata (surface brooder). Sexual products (number of oocytes and spermatic sacs) were quantified, and biochemical characteristics (lipid content and free fatty acid content and composition) were determined to investigate the parental energetic investment and demand in reproduction. Results suggested that the majority of the energetic cost was due to reproductive activity (i.e., gametogenesis and spawning). The two species exhibited different life history strategies, with P. clavata investing more energy in reproduction than C. rubrum. However, P. clavata is reproductively more sensitive to inter-annual changes in environmental conditions.

  4. Estimated reproductive success of brooders and heritability of growth traits for large yellow croaker (Larimichthys crocea) using microsatellites

    Institute of Scientific and Technical Information of China (English)

    LIU Xiande; SUI Banliang; WANG Zhiyong; CAI Mingyi; YAO Cuiluan; CHEN Qingkai


    We estimated the reproductive success of adult brood fish and the heritability of growthrelated traits for large yellow croaker (Larimichthys crocea).We created two independent full-factorial cross groups (1 and 2) by crossing 4 males×4 females and 4 males×3 females,respectively.We measured the body weight (BW),body length (BL),and body height (BH) of 281 individuals from group 1 and 318 individuals from group 2 at 20 months post hatch (harvest age).We also collected a tissue sample from each individual.The parents and offspring were genotyped using six polymorphic microsatellites.Of the 599 offspring,99.2% were assigned to a single pair of parents.In both groups,some parent pairs produced a large number of offsprings while other pairs did not produce any offspring.The genetic diversity and putative Ne were lower in the offsprings than in the parents in both groups.The heritability estimates at 20-month age were 0.13±0.10 for BW,0.19±0.13 for BL,and 0.09±0.06 for BH.The genetic and phenotype correlation between BW,BL,and BH was close to 1.The results provide basic information for selective breeding and further genetic characterization of large yellow croaker.

  5. Effect of maturation degree on fatty acid profile of different tissues in wild and farmed rohu (Labeo rohita)


    Nadeem, Shahid; Hassan, Muhammad; Mahboob, Shahid; Hussain, Bilal; Sultana, Tayyaba


    During the breeding season, lots of physiological changes occur in the bodies of fish which lead to other phenotypical, anatomical and behavioral changes. To evaluate the impact of breeding on the bodily composition of fish, tests were conducted to determine the fatty acid profile of the brooder and Non-brooder wild and farmed fish. Samples were analyzed by gas liquid chromatography using a flame ionization detector.There are non significant differences in the amount of saturated and unsatura...

  6. Effect of maturation degree on fatty acid profile of different tissues in wild and farmed rohu (Labeo rohita

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    Nadeem, Shahid


    Full Text Available During the breeding season, lots of physiological changes occur in the bodies of fish which lead to other phenotypical, anatomical and behavioral changes. To evaluate the impact of breeding on the bodily composition of fish, tests were conducted to determine the fatty acid profile of the brooder and Non-brooder wild and farmed fish. Samples were analyzed by gas liquid chromatography using a flame ionization detector.There are non significant differences in the amount of saturated and unsaturated fatty acids in wild and farmed nonbrooder fish whereas in case of brooder there are significant differences. Brooder farmed fish contained more saturates in gonads approximately twice that of wild fish with C16:0 as the dominating fatty acid. C18:2n-6 and C20:5n-6 were the dominating unsaturated fatty acids in the gonads. Non-brooder fish contained more unsaturated fatty acids in the meat as compared to the brooder fish and farmed fish contained higher amounts of saturated fatty acids as compared to wild fish. C18:1n-9 and C20:4n-6 fatty acids were found in higher quantities and among the saturated acids, C16:0 is the dominating one. More or less similar results were found in the case of the liver fatty acid profile of breeding and nonbreeding fish. Fish of lower weight categories contained more unsaturated fatty acids and so better for consumption to leave the brooders of higher weight categories for the breeding of future generation.Durante la época de reproducción numerosos cambios fisiológicos ocurren en el cuerpo de los peces que guían a cambios anatómicos y de comportamiento. Para evaluar el impacto de la etapa de reproducción en la composición de los peces, ensayos para determinar el perfil de ácidos grasos fueron conducidos en peces salvajes y criados, en dos categorías de peso, maduro (booder o inmaduro (non-brooder. Las muestras fueron analizadas por cromatografía de gases usando un detector de ionización de llama. No hubo

  7. Stress hormones in relation to breeding status and territory location in colonial king penguin: a role for social density? (United States)

    Viblanc, Vincent A; Gineste, Benoit; Stier, Antoine; Robin, Jean-Patrice; Groscolas, René


    Because glucocorticoid (stress) hormones fundamentally affect various aspects of the behaviour, life history and fitness of free-living vertebrates, there is a need to understand the environmental factors shaping their variation in natural populations. Here, we examined whether spatial heterogeneity in breeding territory quality affected the stress of colonial king penguin (Aptenodytes patagonicus). We assessed the effects of local climate (wind, sun and ambient temperature) and social conditions (number of neighbours, distance to neighbours) on the baseline levels of plasma total corticosterone (CORT) in 77 incubating and 42 chick-brooding birds, breeding on territories of central or peripheral colony location. We also assessed the oxidative stress status of a sub-sample of central vs. peripheral chick-brooders to determine whether chronic stress arose from breeding on specific territories. On average, we found that brooders had 55% higher CORT levels than incubators. Regardless of breeding status, central birds experienced greater social density (higher number of neighbours, shorter distance between territories) and had higher CORT levels than peripheral birds. Increasing social density positively explained 40% of the variation in CORT levels of both incubators and brooders, but the effect was more pronounced in brooders. In contrast, climate was similar among breeding territories and did not significantly affect the CORT levels of breeding birds. In brooders, oxidative stress status was not affected by local density or weather conditions. These results highlight that local heterogeneity in breeding (including social) conditions may strongly affect the stress levels of breeding seabirds. The fitness consequences of such variation remain to be investigated.

  8. Dietary L-arginine supplement alleviates hepatic heat stress and improves feed conversion ratio of Pekin ducks exposed to high environmental temperature. (United States)

    Zhu, W; Jiang, W; Wu, L Y


    The current intensive indoor production system of commercial Pekin ducks never allows adequate water for swimming or wetting. Therefore, heat stress is a key factor affecting health and growth of ducks in the hot regions and season. Experiment 1 was conducted to study whether heat stress was deleterious to certain organs of ducks. Forty-one-day-old mixed-sex Pekin ducks were randomly allocated to four electrically heated battery brooders comprised of 10 ducks each. Ducks were suddenly exposed to 37 °C ambient temperature for 3 h and then slaughtered, in one brooder at 21 days and in another brooder at 49 days of age. The results showed that body weight and weight of immune organs, particularly liver markedly decreased in acute heat stress ducks compared with the control. Experiment 2 was carried out to investigate the influences of dietary L-arginine (Arg) supplement on weight and compositions of certain lymphoid organs, and growth performance in Pekin ducks, under daily cyclic hot temperature environment. A total of 151-day-old mixed-sex Pekin ducks were randomly divided into one negative control and two treatment groups, fed experimental diets supplemented with 0, 5, and 10 g L-Arginine (L-Arg)/kg to the basal diet respectively. Ducks were exposed to cyclic high temperature simulating natural summer season. The results showed that the addition of L-Arg improves feed conversion ratio (FCR) during a period of 7-week trial, as well as increases hepatic weight relative to body weight at 21 days, while decreases the hepatic water content at 49 days of age. This study indicated that the liver was more sensitive to acute heat stress, and the hepatic relative weight and chemical composition could be regulated by dietary L-Arg supplementation in Pekin ducks being reared at high ambient temperature. These beneficial effects of Arg on liver might be a cause of improved FCR.

  9. Recent relaxation of purifying selection on the tandem-repetitive early-stage histone H3 gene in brooding sea stars. (United States)

    Foltz, David W; Mah, Christopher L


    Patterns of nucleotide substitution differ between marine species that have a pelagic feeding (planktotrophic) larval stage and related species that lack such a stage, for both adaptive and non-adaptive reasons. Here, patterns of nucleotide and inferred amino acid substitution are analyzed for the tandem-repetitive early-stage histone H3 gene in 36 sea star species of the order Forcipulatida with documented larval habitat. The relative rate of nonsynonymous substitution (expressed as ω=d(N)/d(S)) was significantly higher in lineages with a brooded non-feeding (lecithotrophic) larval form than in lineages with a planktotrophic larval form. There was also a significant excess of conservative over radical substitutions. The increase in ω for brooders as compared to non-brooders was much greater than for previously analyzed mitochondrial sequences in echinoderms. These data are consistent with the hypothesis that purifying selection on this gene has been relaxed in brooding lineages compared to non-brooding lineages. The hypotheses of adaptive or neutral evolution are less plausible, although recent pseudogenization following a period of relaxed purifying selection could also explain the results.

  10. Brooding is Related to Neural Alterations during Autobiographical Memory Retrieval in Aging

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    Sophia Schneider


    Full Text Available Brooding rumination is considered a central aspect of depression in midlife. As older people tend to review their past, rumination tendency might be particularly crucial in late life since it might hinder older adults to adequately evaluate previous events. We scanned 22 non-depressed older adults with varying degrees of brooding tendency with functional magnetic resonance imaging while they performed the construction and elaboration of autobiographical memories. Behavioral findings demonstrate that brooders reported lower mood states, needed more time for memory construction and rated their memories as less detailed and less positive. On the neural level, brooding tendency was related to increased amygdala activation during the search for specific memories and reduced engagement of cortical networks during elaboration. Moreover, coupling patterns of the subgenual cingulate cortex with the hippocampus and the amygdala predicted details and less positive valence of memories in brooders. Our findings support the hypothesis that ruminative thinking interferes with the search for specific memories while facilitating the uncontrolled retrieval of negatively biased self-schemes. The observed neurobehavioral dysfunctions might put older people with brooding tendency at high risk for becoming depressed when reviewing their past. Training of autobiographical memory ability might therefore be a promising approach to increase resilience against depression in late-life.

  11. Norethindrone-induced masculinization and progeny testing in guppy, Poecilia reticulata (Peters 1859). (United States)

    Basavaraja, N; Chandrashekhara, B H; Ahamad, Rather Mansoor


    Norethindrone(NE) was evaluated for its efficacy on alteration of sex ratio of P. reticulata. Either the young fry or the brooders and the resultant fry were fed a commercial diet incorporated with NE at 0, 25, 50, 75 and 100 mg kg(-1) diet (ppm) for 30-40 d in rectangular glass aquaria; this was followed by 40-60 d rearing on NE-free diet in out-door concrete tanks. In general, the androgen treatment altered sex ratio, leading to the production of a dose dependent increase in the percentage of males. The oral administration of the steroid at 75 ppm for 40 d or 100 ppmfor 30 or 40 d to first feeding fry, yielded 100% males. On the other hand, NE administration to brooders before parturition and the resultant fry also produced an all-male population of guppy. The sex ratio of the untreated control was almost 1:1. The survival of fish in all the trials was high, ranging between 67 and 100%. Mating masculinized males ("XX" male) with normal female resulted in an all-female progeny, while crossing normal male (XY) from treatment groups with normal female sired normal sex ratio (1:1), elucidating XX-XY sex determination system in the guppy.

  12. White spot syndrome virus epizootic in cultured Pacific white shrimp Litopenaeus vannamei (Boone) in Taiwan. (United States)

    Cheng, L; Lin, W-H; Wang, P-C; Tsai, M-A; Hsu, J-P; Chen, S-C


    White spot syndrome virus (WSSV) has caused significant losses in shrimp farms worldwide. Between 2004 and 2006, Pacific white shrimp Litopenaeus vannamei (Boone) were collected from 220 farms in Taiwan to determine the prevalence and impact of WSSV infection on the shrimp farm industry. Polymerase chain reaction (PCR) analysis detected WSSV in shrimp from 26% of farms. Juvenile shrimp farms had the highest infection levels (38%; 19/50 farms) and brooder shrimp farms had the lowest (5%; one of 20 farms). The average extent of infection at each farm was as follows for WSSV-positive farms: post-larvae farms, 71%; juvenile farms, 61%; subadult farms, 62%; adult farms, 49%; and brooder farms, 40%. Characteristic white spots, hypertrophied nuclei and basophilic viral inclusion bodies were found in the epithelia of gills and tail fans, appendages, cephalothorax and hepatopancreas, and virions of WSSV were observed. Of shrimp that had WSSV lesions, 100% had lesions on the cephalothorax, 96% in gills and tail fans, 91% on appendages and 17% in the hepatopancreas. WSSV was also detected in copepoda and crustaceans from the shrimp farms. Sequence comparison using the pms146 gene fragment of WSSV showed that isolates from the farms had 99.7-100% nucleotide sequence identity with four strains in the GenBank database--China (AF332093), Taiwan (AF440570 and U50923) and Thailand (AF369029). This is the first broad study of WSSV infection in L. vannamei in Taiwan.

  13. Effect of artificial rearing of piglets on the volume densities of M cells in the tonsils of the soft palate and ileal Peyer's patches. (United States)

    Prims, S; Pintens, N; Vergauwen, H; Van Cruchten, S; Van Ginneken, C; Casteleyn, C


    The high prolificacy of modern hybrid sows has increased the mean litter size during the last decades. However, rearing large litters is challenging and has increased the use of alternative management strategies such as euthanasia of weak piglets, cross-fostering, supplementing piglets with milk, split-nursing and split-weaning. The latter includes artificial rearing on brooders where piglets have ad libitum access to milk replacer. The effect of this artificial rearing on the immune system of the piglet is the subject of various studies. The present study focused on the M cells in the tonsil of the soft palate and in the ileal Peyer's patch (iPP). These epithelial cells are specialized in antigen sampling and play a pivotal role in the induction of adaptive immune responses. The volume densities of the M cells were assessed by stereological analysis of tissue samples from piglets of 0, 3, 8 and 19days of age. During the first three days, piglets suckled the sow, permitting them to ingest colostrum. At the third day, the piglets were either allowed to continue to suckle the sow or were transferred to brooders. The six experimental groups, each containing six piglets, thus consisted of newborn piglets, 3-day-old sow-suckled piglets, and conventionally and artificially reared piglets of 8 and 19days of age. To identify M cells, tissue samples were immersed in 4% phosphate-buffered paraformaldehyde and paraffin sections were immunohistochemically stained against cytokeratin 18. The volume densities of M cells in both the crypt epithelium of the tonsils of the soft palate and the follicle-associated epithelium of the iPPs did not show any difference between the various age groups of conventionally reared piglets. However, values were twice as high in the iPPs compared to the tonsils of the soft palate. In contrast, a decrease in volume densities of M cells was observed in the iPPs of piglets after they had been transferred to commercial brooders (P=0.05), resulting in

  14. Differential larval settlement responses of Porites astreoides and Acropora palmata in the presence of the green alga Halimeda opuntia (United States)

    Olsen, K.; Sneed, J. M.; Paul, V. J.


    Settlement is critical to maintaining coral cover on reefs, yet interspecific responses of coral planulae to common benthic macroalgae are not well characterized. Larval survival and settlement of two Caribbean reef-building corals, the broadcast-spawner Acropora palmata and the planulae-brooder Porites astreoides, were quantified following exposure to plastic algae controls and the green macroalga Halimeda opuntia. Survival and settlement rates were not significantly affected by the presence of H. opuntia in either species. However, ~10 % of P. astreoides larvae settled on the surface of the macroalga, whereas larvae of A. palmata did not. It is unlikely that corals that settle on macroalgae will survive post-settlement; therefore, H. opuntia may reduce the number of P. astreoides and other non-discriminatory larvae that survive to adulthood. Our results suggest that the presence of macroalgae on impacted reefs can have unexpected repercussions for coral recruitment and highlight discrepancies in settlement specificity between corals with distinct life history strategies.

  15. Genetic population structure in the Antarctic benthos: insights from the widespread amphipod, Orchomenella franklini. (United States)

    Baird, Helena Phoenix; Miller, Karen Joy; Stark, Jonathan Sean


    Currently there is very limited understanding of genetic population structure in the Antarctic benthos. We conducted one of the first studies of microsatellite variation in an Antarctic benthic invertebrate, using the ubiquitous amphipod Orchomenella franklini (Walker, 1903). Seven microsatellite loci were used to assess genetic structure on three spatial scales: sites (100 s of metres), locations (1-10 kilometres) and regions (1000 s of kilometres) sampled in East Antarctica at Casey and Davis stations. Considerable genetic diversity was revealed, which varied between the two regions and also between polluted and unpolluted sites. Genetic differentiation among all populations was highly significant (F(ST) = 0.086, R(ST) = 0.139, pbenthos. These results provide insights into processes of speciation in Antarctic brooders, and will help inform the design of spatial management initiatives recently endorsed for the Antarctic benthos.

  16. Genetic mating systems and reproductive natural histories of fishes: lessons for ecology and evolution. (United States)

    Avise, John C; Jones, Adam G; Walker, DeEtte; DeWoody, J Andrew


    Fish species have diverse breeding behaviors that make them valuable for testing theories on genetic mating systems and reproductive tactics. Here we review genetic appraisals of paternity and maternity in wild fish populations. Behavioral phenomena quantified by genetic markers in various species include patterns of multiple mating by both sexes; frequent cuckoldry by males and rare cuckoldry by females in nest-tending species; additional routes to surrogate parentage via nest piracy and egg-thievery; egg mimicry by nest-tending males; brood parasitism by helper males in cooperative breeders; clutch mixing in oral brooders; kinship in schooling fry of broadcast spawners; sperm storage by dams in female-pregnant species; and sex-role reversal, polyandry, and strong sexual selection on females in some male-pregnant species. Additional phenomena addressed by genetic parentage analyses in fishes include clustered mutations, filial cannibalism, and local population size. All results are discussed in the context of relevant behavioral and evolutionary theory.

  17. Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. (United States)

    Panijpan, Bhinyo; Kowasupat, Chanon; Laosinchai, Parames; Ruenwongsa, Pintip; Phongdara, Amornrat; Senapin, Saengchan; Wanna, Warapond; Phiwsaiya, Kornsunee; Kühne, Jens; Fasquel, Frédéric


    Fighting fish species in the genus Betta are found in several Southeast Asian countries. Depending on the mode of paternal care for fertilized eggs and hatchlings, various species of the betta fish are classified as mouth brooders or nest builders whose members in turn have been grouped according to their similarities mainly in morphology. The mouth brooders as well as some nest builders involved in the present study include fishes discovered and identified subsequent to previous reports on species groupings and their positions on phylogenetic trees based on DNA sequences that differ from those used by us in this study. From the mitochondrial COI gene and nuclear ITS1 gene sequences and more accurate analyses we conclude that the following members of the mouth-brooding pairs, named differently previously, are virtually identical, viz the Betta prima-Betta pallida pair and Betta ferox-Betta apollon pair. The Betta simplex, hitherto believed to be one species, could possibly be genetically split into 2 distinct species. In addition, several other established type-locality fishes could harbor cryptic species as judged by genetic differences. Assignments of fish species to groups reported earlier may have to be altered somewhat by the present genetic findings. We propose here a new Betta fish phylogenetic tree which, albeit being similar to the previous ones, is clearly different from them. Our gene-based evidence also leads to assignments of some fishes to new species groups and alters the positions of some species on the new phylogenetic tree, thus implying different ancestral relationships.

  18. Embryonic development, hatching, mineral consumption, and survival of Macrobrachium rosenbergii (de Man reared in artificial seawater in closed recirculating water system at different levels of salinity

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    Krasindh Hangsapreurke


    Full Text Available This experiment aims to study the effect of different levels of salinity (5, 15 and 25 ppt using artificial sea water on the embryonic development and hatching percentage of the eggs of unripe berried female giant freshwater prawns (Macrobrachium rosenbergii with an average size of 14.3+0.6 cm TL. After incubation through the heart beating stage (grayish black eggs, the brooders in each salinity were separately transferred to the hatching tank with 15 ppt saline water for the second part of the study. After hatching, the healthy larvae from the brooders which were previously incubated in 3 levels of salinity were collected for the larviculture experiment. The closed recirculating water system with trickling filter unit packed with fiberglass and bioballs was used as incubation and larviculture units. The metamorphosis period and survival rate were examined. The rearing water from each larviculture aquarium was collected for determination of sodium, magnesium, potassium, calcium and chloride ions. The result showed that the percentage of ripe berried females (with heart beating stage embryos were not significantly different (p>0.05 between 5 and 15 ppt salinity but their values were significantly higher (p0.05 in the survival rate of post larvae and metamorphosis period among the treatments. The first post larvae stage occurred on the 26th day. During 30 days of larviculture, the survival rate of all treatments was 100% until the 19th day, after which it suddenly decreased. When the concentrations of the ions in the rearing water were determined in all treatments, it was found that the concentration of magnesium ions rapidly declined (p0.05. The low survival rate during the final stage of larviculture might be due to the depletion of the previously mentioned ions especially that of magnesium.

  19. Divergence between Antarctic and South American marine invertebrates: What molecular biology tells us about Scotia Arc geodynamics and the intensification of the Antarctic Circumpolar Current (United States)

    Poulin, Elie; González-Wevar, Claudio; Díaz, Angie; Gérard, Karin; Hüne, Mathias


    Continental drift processes such as major gateway openings have been historically advocated to explain the distribution of marine benthic taxa in the Southern Ocean (SO). The separation between Antarctic Peninsula and the southern tip of South America together with the onset of the Antarctic Circumpolar Current (ACC) represent the final step for the complete isolation of the Antarctic region. However, there is still controversy concerning the timing and mode of this process, and especially about the role of the Scotia Arc geodynamics in the development of a fully deep and intensified ACC circulation. Based on mitochondrial Cytochrome c Oxidase Subunit I (COI) sequences obtained from different taxa, we performed molecular comparisons between Antarctic and South American relatives to provide independent time estimations of Antarctica's isolation. We include in the analyses congeneric Antarctic and Patagonian near-shore marine benthic invertebrates including indirect developers (Nacella, Yoldia, Sterechinus, and Parbolasia) and brooders (Xymenopsis and Trophonella). Considering the levels of genetic differentiation between relatives from both regions and assuming the molecular clock hypothesis, we estimated the onset of their respective divergence. On one hand, similar levels of genetic distance in broadcast-spawners (7%-8.3%) support the hypothesis that the development of an effective barrier between Antarctica and South America occurred almost simultaneously for these groups. Divergence time estimations based on specific substitution rates indicate that the separation occurred near the Mio-Pliocene transition, long after the physical separation of both continents. Genetic distance and divergence time estimation in direct developers indicate an older separation time, close to the mid-Miocene. Even when the analyzed groups included both broadcast-spawners and brooder organisms, the divergence between Antarctic and South America lineages rather than being related to

  20. Nutritional implications of pectins in chicks in relation to esterification and origin of pectins. (United States)

    Langhout, D J; Schutte, J B


    An experiment was conducted to examine the effect of feeding high-methylated (HM) and low-methylated (LM) pectin on performance and physiological characteristics in broiler chicks. Two levels each (1.5 and 3%) of HM citrus pectin (HMC), LM citrus pectin (LMC), or HM sugar beet pectin (HMS) were added to a semi-purified basal diet. The experiment was conducted in battery brooders, and chicks received the diets as pellets from 6 to 27 d of age. The inclusion of LMC or HMS in the diet had little or no effect on the performance of chicks; however, when HMC was added to the diet growth and feed utilization were reduced significantly (P Waterholding capacity of the diets and the excreta were increased significantly by including one (P 0.05) on the concentration of VFA. In contrast, inclusion of HMS in the diet significantly increased the concentration of VFA in the cecal content (P < 0.05) dose-dependently. Based on the results of the present study, it can be concluded that the effect of dietary pectin on chick performance is dependent on the degree of carboxyl groups that is esterified, the origin of the pectin product, and the amount added to the diet.

  1. To brood or not to brood: Are marine invertebrates that protect their offspring more resilient to ocean acidification? (United States)

    Lucey, Noelle Marie; Lombardi, Chiara; Demarchi, Lucia; Schulze, Anja; Gambi, Maria Cristina; Calosi, Piero


    Anthropogenic atmospheric carbon dioxide (CO2) is being absorbed by seawater resulting in increasingly acidic oceans, a process known as ocean acidification (OA). OA is thought to have largely deleterious effects on marine invertebrates, primarily impacting early life stages and consequently, their recruitment and species’ survival. Most research in this field has been limited to short-term, single-species and single-life stage studies, making it difficult to determine which taxa will be evolutionarily successful under OA conditions. We circumvent these limitations by relating the dominance and distribution of the known polychaete worm species living in a naturally acidic seawater vent system to their life history strategies. These data are coupled with breeding experiments, showing all dominant species in this natural system exhibit parental care. Our results provide evidence supporting the idea that long-term survival of marine species in acidic conditions is related to life history strategies where eggs are kept in protected maternal environments (brooders) or where larvae have no free swimming phases (direct developers). Our findings are the first to formally validate the hypothesis that species with life history strategies linked to parental care are more protected in an acidifying ocean compared to their relatives employing broadcast spawning and pelagic larval development.

  2. Sublethal Effects of Methanolic Extract of Raphia hookeri on the Reproductive Capacity of Clarias gariepinus

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    Adedotun O. Afolayan


    Full Text Available Raphia hookeri fruits are used for fishing in Nigeria due to their ichthyotoxic properties. This study investigated the toxic effects of R. hookeri on the reproductive capacity of Clarias gariepinus. The results from both short-term (96-hour test and long-term (3-month sublethal test bioassays revealed a linear relationship between R. hookeri extract dose and negative effects on the catfish. The percentage survival of both sexes of the catfish decreased with increasing extract concentration at short-term exposure, with LC50 values of 600 mg/L and 800 mg/L for male and female, respectively. At long-term exposure, the reproductive capacity of 10–12-month-old male and female brood-stocks diminished at relatively higher concentrations of R. hookeri fruit extract, with the gravid females producing fewer and mostly unviable eggs. The fruit extract also affected the eggs’ hatchability and fry survival when the exposed gravid females were treated with pituitary hormone and sperms from unexposed males, while the exposed males were unable to sexually stimulate female brooders. Sperms and pituitary hormone from exposed males were infertile, leading to low percentage of hatched eggs and mortality of the few hatched fries within 24 hours. These results confirmed the ethnobotanical use of this fruit extract for fishing in Nigeria.


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    Bambang Iswanto


    Full Text Available Characteristics of the Egyptian African catfish (Clarias gariepinus strain introduced to Indonesia has not been extensively explored yet, especially the red strain. Previous studies suggested that at the same body length, body weight of the red strain was higher and it was more rotund than that of the normal (black ones. These differences need to be further investigated to find out which parts of the body mainly contributed to shape the differences. The present study was carried out to explore morphological differences of the red strain of Egyptian African catfish compared to the black strain through morphometric and meristic characterizations. Meristic and morphometric characterizations in the present study were carried out following standard method for morphological characterization of Clarias catfish. The fish samples consisted of each 35 red and black table-sized fish samples resulted from inbred and outbred spawnings. Results of the morphometric and meristic analysis in the present study revealed that the red strain of Egyptian African catfish resulted from inbred spawning of red strain brooders was morphologically different from that of either parental fishes or the black strains. At the same body length, head of the red strain was bigger (wider and longer than other strains, and its body was stumpy (more rotund and shorter than other strains, deviated from those normal characteristics of the Egyptian African catfish. Its meristic characters were also differed from those of other strains, assigned by reduced dorsal and anal fin rays number.

  4. If I only knew why: the relationship between brooding, beliefs about rumination, and perceptions of treatments. (United States)

    Ophir, Yaakov; Mor, Nilly


    People who tend to engage in brooding, the maladaptive subtype of rumination, are at risk to develop depression. Brooders often endorse metacognitive beliefs that self-focused ruminative thinking is beneficial. In the current study, we examined whether brooding and positive beliefs about rumination are associated with perceptions of and preferences for treatments for depression. Participants (N=118) read descriptions of two different clusters of treatments for depression, Insight-Oriented (IO) treatments and Activation-Oriented (AO) treatments. They then rated treatment efficacy and credibility and completed self-report measures of rumination (including brooding and reflection subscales), beliefs about rumination, and depression. Brooding and metacognitive positive beliefs about rumination were associated with positive perceptions of IO (but not AO) treatments. Positive beliefs about rumination contributed to the prediction of perceptions of IO treatments (but not AO treatments) beyond the effect of brooding. We discuss the implications of these findings for individuals' decision-making processes regarding which type of treatment to seek.

  5. Evaluation of dietary supplements of lipase, detergent, and crude porcine pancreas on fat utilization by young broiler chicks. (United States)

    Al-Marzooqi, W; Leeson, S


    The purpose of Experiment 1 was to improve the digestibility of fat through the use of supplemental lipase enzymes. A 2 x 3 factorial arrangement of treatments involving two levels of animal-vegetable blend fat (AV) (4 and 8%) and three enzyme treatments, namely none; Pancreatic, 0.714%; and Pancreatin, 0.714%, were randomly allocated within a battery brooder. There was an increase in diet ME and apparent fat digestibility when Pancreatic and Pancreatin enzymes were used (P 0.05). Experiment 4 was conducted to test the effect of supplementation of graded levels of ground crude porcine pancreas at 0, 0.321, 0.535, 0.750, 0.964, 1.178, or 1.392% of the diet on performance of male broiler chicks to confirm the anorexic effect caused by supplementing with Pancreatic enzyme. In general, there was no significant effect of feeding crude porcine pancreas on the performance of male broiler chicks (P > 0.05). In these studies, lipase enzymes improved fat digestion, although it is suspected that associated reduced feed intake may be associated with contaminants such as cholecystokinin hormone.

  6. Genetic population structure in the Antarctic benthos: insights from the widespread amphipod, Orchomenella franklini.

    Directory of Open Access Journals (Sweden)

    Helena Phoenix Baird

    Full Text Available Currently there is very limited understanding of genetic population structure in the Antarctic benthos. We conducted one of the first studies of microsatellite variation in an Antarctic benthic invertebrate, using the ubiquitous amphipod Orchomenella franklini (Walker, 1903. Seven microsatellite loci were used to assess genetic structure on three spatial scales: sites (100 s of metres, locations (1-10 kilometres and regions (1000 s of kilometres sampled in East Antarctica at Casey and Davis stations. Considerable genetic diversity was revealed, which varied between the two regions and also between polluted and unpolluted sites. Genetic differentiation among all populations was highly significant (F(ST = 0.086, R(ST = 0.139, p<0.001 consistent with the brooding mode of development in O. franklini. Hierarchical AMOVA revealed that the majority of the genetic subdivision occurred across the largest geographical scale, with N(em≈1 suggesting insufficient gene flow to prevent independent evolution of the two regions, i.e., Casey and Davis are effectively isolated. Isolation by distance was detected at smaller scales and indicates that gene flow in O. franklini occurs primarily through stepping-stone dispersal. Three of the microsatellite loci showed signs of selection, providing evidence that localised adaptation may occur within the Antarctic benthos. These results provide insights into processes of speciation in Antarctic brooders, and will help inform the design of spatial management initiatives recently endorsed for the Antarctic benthos.

  7. Impact of seasonal variation and feeding on reproductive behavior of fresh water spiny eel Mastacembelus armatus from Cauvery River

    Institute of Scientific and Technical Information of China (English)

    Venkatachalam Uthayakumar; Parathattil Rathan Sreedevi; Dhanapalan Senthilkumar; Subramanian Munirasu; Aathi Kiruba; Venkatachalam Ramasubramanian


    Objective: To analyze the effect of seasonal variation and nutritional factors over the fecundity, gonado somatic index (GSI) and sperm motility of fresh water spiny eel Mastacembelus armatus (M. armatus) under artificial cave fitted tanks in laboratory condition. Methods: Forty pairs of M. armatus were collected from River Cauvery and are acclimated under lab condition. The fishes were fed with three different feeds like Fresh water prawns, earthworms and fish larvae for 60 days. Fecundity, sperm motility, Length weight relationship and GSI were analysed using standard procedures. Results: The fecundity rate was increased when body weight and gonad weight increased. The lograthemic relationship of the fecundity-total length and fecundity-body weight were found to be more correlated than the fecundity-gonad weight relationship. Maximum GSI was recorded in both male and female fishes during the breeding season (June-October). The condition factor (K) was maximum at the non breeding seasons and reduced in peak breeding season of M. armatus. In male fishes the sperm motility was recorded maximum (70%-85%) in the breeding season and regressed gradually to 0-15%. Among the feeding groups earthworm fed group showed higher body weight and fecundity rate than the control group which was statistically significant at P <0.05 level. Conclusion: The outcome of the present study shows the seasonal variation and feeding increase the reproductive performance. It can be effectively used for the healthy brooder selection, captive breeding and conservation of M. armatus.


    Directory of Open Access Journals (Sweden)

    B. Indarsih


    Full Text Available The study was conducted as a completely randomized design, with a factorial arrangement to determine the response of commercial broilers to choice feeding and limiting amino acids on growth and carcass performance. A total of 432 male birds were weighed at one-d-old and randomly distributed to 48 wire-floored brooder cage each 1.0 m2. There were 2 sexes and 4 dietary treatments with 6 replicates each of 9 birds. Birds were given one of three dietary regimens with dietary change every 7 days. All groups were fed free choice of summit and dilution diets. The estimated dietary level of crude protein at day-old was 240 g/kg and the level at 42 d was either 120, 150 or 180 g/kg for females or 130, 160 and 190 g/kg for males. At 43 d of age, all birds from each dietary treatment were slaughtered for measurement of body composition. Results reveal that lysine requirement for maximum gain in this study was higher than NRC recommendation. The free choice-fed bird was significantly higher, in terms of growth and body composition than that obtained on the low dietary protein regimen.Keyword

  9. Impact of L-phenylalanine supplementation on the performance of three-week-old broilers fed diets containing ochratoxin A. 2. Effects on hematology and clinical chemistry. (United States)

    Bailey, C A; Gibson, R M; Kubena, L F; Huff, W E; Harvey, R B


    Phenylalanine was evaluated for its ability to protect broiler chickens from the toxic effects of ochratoxin A (OA). A completely randomized 2-by-3 factorial design was utilized consisting of 0, .8, and 2.4% supplemental L-phenylalanine (Phe) and of 0 and 4 mg of OA per kg of diet. The basal diet contained 14% protein. Broilers were raised in battery brooders to 3 wk of age, when blood was collected and various hematological parameters were determined. The health status of the broilers was evaluated by assaying serum for various enzyme activities and metabolites using an automated, clinical chemistry analyzer. Adding OA to the broiler diets resulted in an increased concentration of serum hemoglobin as well as increased activity for cholinesterase and gamma glutamyl transferase but in decreased activity for aspartate amino transferase, lactate dehydrogenase, and alkaline-phosphatase activity as well as decreased concentrations of total triglyceride and of inorganic phosphorus. Supplemental Phe decreased the concentrations of hemoglobin and serum glucose. The regression slopes for Phe at 4 mg of OA per kg of diet were significant for uric acid, creatinine, total protein, albumin, and cholesterol suggesting that supplemental Phe improved the health status of the broilers fed diets containing OA with respect to these parameters.

  10. Social status, breeding state, and GnRH soma size in convict cichlids (Cryptoheros nigrofasciatus). (United States)

    Chee, San-San Amy; Espinoza, Walter A S; Iwaniuk, Andrew N; Pakan, Janelle M P; Gutiérrez-Ibáñez, Cristian; Wylie, Douglas R; Hurd, Peter L


    Gonadotropin-releasing hormone (GnRH) expressing neurons in the preoptic area (POA) of the hypothalamus plays a key role in regulating reproductive function through the control of gonadotropin release. Several studies have illustrated the importance of the social environment in modulating the size of GnRH expressing neurons. In the African cichlid fish Astatotilapia burtoni, the size of the soma of GnRH expressing neurons in the POA varies with social status in males, and with breeding state in females. Territorial males have larger GnRH+ cells than non-territorial males, while brooder females have smaller GnRH+ cells than control females. The lek-like breeding system of A. burtoni is, however, only one type of social system within the diverse assemblage of cichlids. To gain a better understanding of GnRH neuronal plasticity in response to the changes in the social environment, we tested whether similar effects occur in the monogamous New World cichlid, the convict cichlid (Cryptoheros nigrofasciatus), a model species for the study of social behaviour. Our results indicate that, indeed GnRH expressing neuron soma size, and not cell number, varies with both male territorial status, and manipulations of female breeding state in this monogamous, biparental, New World cichlid.

  11. Aracia sinaloae sp. n., a new brooding, simultaneous hermaphroditic fan worm from southern Gulf of California (Polychaeta: Sabellidae). (United States)

    Tovar-Hernández, María Ana


    Currently, only two species are known in the sabellid genus Aracia: A. riwo (Rouse, 1996) and A. heterobranchiata (Nogueira, López & Rossi, 2004). The first was described from the surface of a Teredo-riddled log lying in 1 m of water among mangrove roots, in Papua (New Guinea), whilst the second was found on an organically enriched beach at São Sebastião (Brazil). In this contribution, a third species of Aracia is described from settling panels and red mangrove roots in an estuarine system located in southern Gulf of California (Mexico). The new species is a simultaneous hermaphrodite and brooder, unique in the presence of a rectangular ventral shield on the collar, shorter than those in posterior thoracic segments; and a high number of rows of teeth in thoracic and abdominal uncini, covering from one-half to three-quarters of the main fang length. Information about color patterns in live specimens, glandular patterns, and reproduction is provided.

  12. Seasonality and lunar periodicity in the sexual reproduction of the coral-killing sponge, Terpios hoshinota (United States)

    Nozawa, Yoko; Huang, Yu-Sin; Hirose, Euichi


    Sexual reproduction of the cyanobacteriosponge, Terpios hoshinota, was studied at Lyudao (Green Island), Taiwan, from 2011 to 2013 through histological examinations, electron microscopy, and in situ observations of larval release. Histological examinations identified five reproductive structures: oocytes, sperm cysts, cell masses, early-stage embryos, and mature embryos. These reproductive structures were often observed in the same specimens, indicating that T. hoshinota is a hermaphroditic brooder. No cyanobacteria were seen in the gametes, and transmission of symbiotic cyanobacteria from parental tissues to larvae likely occurred during embryogenesis. The cell mass, a loose aggregate of numerous symbiotic cyanobacteria and maternal sponge cells, appeared to be eventually incorporated and constitutes the inner part of pre-hatching larvae, suggesting that the larval type is pseudoblastula instead of parenchymella as previously suggested. A clear lunar periodicity was seen in the reproductive cycle; larvae were released mostly around the full moon and occasionally around the new moon. Reproductive activity declined during months with low temperatures (January-April). The larvae were characterized by negative buoyancy and limited mobility, suggesting a larval dispersal distance on a scale of meters under calm weather. However, long-distance dispersal of larvae could still be possible through occasional strong currents during bad weather, such as typhoons, if the larvae survived during the dispersal period by accessing extra energy from the symbiotic cyanobacteria through their photosynthates and/or consuming the cyanobacteria as a source of nutrients. This study showed that T. hoshinota has high fecundity, with a monthly release of numerous larvae over a long reproductive season. This high fecundity, along with local larval dispersal and rapid post-settlement growth, enables rapid population expansion of T. hoshinota.

  13. The acquisition and internalization of Salmonella by the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae). (United States)

    Crippen, Tawni L; Sheffield, Cynthia L; Esquivel, Sharon V; Droleskey, Robert E; Esquivel, Jesus F


    In poultry broiler production facilities, it is important to understand the sources and contribution of reservoir populations of pathogens. The lesser mealworm beetle, Alphitobius diaperinus (Panzer), is a common pest in poultry litter that is reported to carry pathogens affecting both human and animal health. This study investigates whether the carriage of a bacterial pathogen occurs by the harboring of bacteria internally by these insects. Beetles were exposed to a marker bacterium, Salmonella enterica serovar Typhimurium-green fluorescent protein (ST-GFP), at concentrations up to 10(7) colony-forming units (cfu)/mL for 0.5 to 12 h, and then subsequently surface disinfected and dissected. The head, gastrointestinal tract and hemolymph were cultured for the presence of ST-GFP. This study definitively demonstrates the internal carriage of Salmonella by this insect and found that the beetles rapidly acquired bacteria from external sources and harbored the bacteria within their alimentary canal after exposure for 30 min at 10(4) cfu/mL and within the hemolymph after exposure for 2 h at 10(6) cfu/mL. Beetles internalized an average of 9.5 × 10(1) and 3.2 × 10(3) after a 2-h exposure to 2 × 10(4) and 2 × 10(6) cfu/mL, respectively. The lesser mealworm is a serious pest within the poultry brooder and laying industry and because of their mobility, voracious feeding habits, and prey potential may represent an active source facilitating the dissemination of Salmonella.

  14. Altered Reproduction in Clarias gariepinus Exposed to Industrial Effluents

    Directory of Open Access Journals (Sweden)

    Adeogun A. Olubukola


    Full Text Available Problem statement: Reproductive success is a key factor in determining species survival and the unregulated discharge of industrial effluents into rivers and streams in a developing country like Nigeria, can impair reproductive ability in non-target organisms like fish. Fish, generally accumulate contaminants from their environment in their tissues and these can be transmitted through the yolk lipids to the eggs. Approach: Prespawning Clarias gariepinus female brooders were exposed to composite effluent mixtures from a food and beverage industry (discharged into Ona river for a period of thirty days to determine its effect on growth and fertility/reproductive success (viability of eggs and survival of the F1 generation fries. Physico-chemical parameters of effluent samples and exposure concentrations were analyzed and effluent sample was further analyzed for heavy metals (Fe, Pb, Cd, Ni, Zn, Cu, Mn. Results: Exposure to the effluents caused decreased weight (-7.33% and egg production (198 eggs in the highest exposure concentration. Significant decreases (p1 generation fries (68.68, 46.42 and 22.33% with increasing effluent concentration was also observed in the order 0.00%> 4.40%> 6.60% and there were no viable eggs and fingerlings in the highest exposure concentration(13.20%. Effluent sample and exposure concentrations had acidic pH and low DO values while effluent sample contained Fe and Pb in concentrations above the specified standard by the National Environment Standards and Regulation Agency (NESREA for food and beverage industry effluent discharged into surface waters. Conclusion: The toxic nature of these effluents on the reproductive success of C. gariepinus (a commercially important freshwater fish in Nigeria is of great concern to sustainable fisheries if stringent measures to monitor effluent quality discharge into this river are not implemented.

  15. Blind to morphology: Genetics identifies several widespread ecologically common species and few endemics among Indo-Pacific cauliflower corals (Pocillopora, Scleractinia)

    KAUST Repository

    Pinzón, Jorge H C


    Aim: Using high-resolution genetic markers on samples gathered from across their wide distributional range, we endeavoured to delimit species diversity in reef-building Pocillopora corals. They are common, ecologically important, and widespread throughout the Indo-Pacific, but their phenotypic plasticity in response to environmental conditions and their nearly featureless microskeletal structures confound taxonomic assignments and limit an understanding of their ecology and evolution. Location: Indo-Pacific, Red Sea, Arabian/Persian Gulf. Methods: Sequence analysis of nuclear ribosomal (internal transcribed spacer 2, ITS2) and mitochondrial (open reading frame) loci were combined with population genetic data (seven microsatellite loci) for Pocillopora samples collected throughout the Indo-Pacific, Red Sea and Arabian Gulf, in order to assess the evolutionary divergence, reproductive isolation, frequency of hybridization and geographical distributions of the genus. Results: Between five and eight genetically distinct lineages comparable to species were identified with minimal or no hybridization between them. Colony morphology was generally incongruent with genetics across the full range of sampling, and the total number of species is apparently consistent with lower estimates from competing morphologically based hypotheses (about seven or eight taxa). The most commonly occurring genetic lineages were widely distributed and exhibited high dispersal and gene flow, factors that have probably minimized allopatric speciation. Uniquely among scleractinian genera, this genus contains a monophyletic group of broadcast spawners that evolved recently from an ancestral brooder. Main conclusions: The delineation of species diversity guided by genetics fundamentally advances our understanding of Pocillopora geographical distributions, ecology and evolution. Because traditional diagnostic features of colony and branch morphology are proving to be of limited utility, the

  16. Early life history and spatiotemporal changes in distribution of the rediscovered Suwannee moccasinshell Medionidus walkeri (Bivalvia: Unionidae) (United States)

    Johnson, Nathan A.; Mcleod, John; Holcomb, Jordan; Rowe, Matthew T.; Williams, James D.


    Accurate distribution data are critical to the development of conservation and management strategies for imperiled species, particularly for narrow endemics with life history traits that make them vulnerable to extinction. Medionidus walkeri is a rare freshwater mussel endemic to the Suwannee River Basin in southeastern North America. This species was rediscovered in 2012 after a 16-year hiatus between collections and is currently proposed for listing under the Endangered Species Act. Our study fills knowledge gaps regarding changes in distribution and early life history requirements of M. walkeri. Spatiotemporal changes in M. walkeri distribution were displayed using a conservation status assessment map incorporating metadata from 98 historical (1916–1999) and 401 recent (2000–2015) site surveys from museums and field notes representing records for 312 specimens. Recent surveys detected M. walkeri only in the middle Suwannee subbasin (n = 86, 22 locations) and lower Santa Fe subbasin (n = 2, 2 locations), and it appears the species may be extirpated from 67% of historically occupied 10-digit HUCs. In our laboratory experiments, M. walkeri successfully metamorphosed onPercina nigrofasciata (56.2% ± 8.9) and Etheostoma edwini (16.1% ± 7.9) but not on Trinectes maculatus, Lepomis marginatus, Notropis texanus, Noturus leptacanthus, Etheostoma fusiforme, orGambusia holbrooki. We characterize M. walkeri as a lure-displaying host fish specialist and a long-term brooder (bradytictic), gravid from fall to early summer of the following year. The early life history and distribution data presented here provide the baseline framework for listing decisions and future efforts to conserve and recover the species.

  17. Life history and propagation of the endangered dromedary pearlymussel (Dromus dromas) (Bivalvia:Unionidae) (United States)

    Jones, J.W.; Neves, R.J.; Ahlstedt, S.A.; Mair, R.A.


    The reproduction, demography, and propagation of the endangered dromedary pearlymussel (Dromus dromas) (Lea, 1834) were studied in the Clinch and Powell rivers, Tennessee. Viable populations of the dromedary pearlymussel now occur only in the Clinch and Powell rivers; the species has been extirpated from the remaining portions of its range in the Cumberland and Tennessee river drainages. Females are long-term winter brooders, and they are gravid from October to June. Glochidia are contained in conglutinates that are red to white and resemble freshwater leeches or flatworms. Conglutinates are 20 to 40 mm long and are released through the excurrent aperture. Estimates of fecundity based on 7 gravid females collected from the Clinch River were 55,110 to 253,050 glochidia/mussel. The ages of 66 valves of D. dromas were determined by thin-sectioning and ranged from 3 to 25 y. Annual growth averaged 5 mm/y until age 10 and decreased to ???1.2 mm/ y thereafter. Nineteen fish species were tested for suitability as hosts for glochidia. Ten were confirmed as hosts through induced infestations of glochidia: black sculpin (Cottus baileyi), greenside darter (Etheostoma blennioides), fantail darter (Etheostoma flabellare), snubnose darter (Etheostoma simoterum), tangerine darter (Percina aurantiaca), blotchside logperch (Percina burtoni), logperch (Percina caprodes), channel darter (Percina copelandi), gilt darter (Percina evides), and Roanoke darter (Percina roanoka). Juveniles produced from these hosts were cultured in dishes held in nonrecirculating aquaculture systems containing fine sediment (<105 ??m) and were fed the green alga Nannochloropsis oculata every 2 d. Survival of 2810 newly metamorphosed juveniles was 836 (29.7%) after 1 to 2 wk.

  18. Effect of Origanum chemotypes on broiler intestinal bacteria. (United States)

    Betancourt, Liliana; Rodriguez, Fernando; Phandanouvong, Vienvilay; Ariza-Nieto, Claudia; Hume, Michael; Nisbet, David; Afanador-Téllez, German; Van Kley, Alexandra Martynova; Nalian, Armen


    Essential oils have been proposed as alternatives to antibiotic use in food animal production. This study evaluated 3 chemotypes of the Origanum genus, containing varying amounts of secondary metabolites carvacrol, thymol, and sabinene, in the broiler chicken diet. Aerial parts of Origanum vulgare L. (OL), O. vulgare L. ssp. hirtum (OH), and O. majorana (OM) were collected from a greenhouse located in the high altitude Sabana de Bogotá (Savanna of Bogotá) and O. vulgare L. ssp. hirtum (OG) produced and ground in Greece. Oregano essential oils (OEO) from these plants were obtained by steam distillation and analyzed by gas chromatography coupled to a mass spectrometer. Six treatments were evaluated: 200 mg/kg of OEO from OH, OL, and OM, 50 mg/kg of OEO from OG, 500 mg/kg of chlortetracycline, and without additives. Broiler chicks were maintained at 2,600 m above sea level, placed in brooder cages under a completely randomized design. Template DNA was isolated from duodenal, jejunal, ileal, and cecal contents in each group and bacterial 16S rDNA patterns were analyzed by denaturing gradient gel electrophoresis. Dendrograms of denaturing gradient gel electrophoresis band patterns revealed 2 main clusters, OEO-treated chicks and nontreated control chicks, in each intestinal segment. Band patterns from different gut compartments revealed major bacterial population shifts in the foregut (duodenum, jejunum, and ileum) compared with the hindgut (cecum and colon) at all ages evaluated (P < 0.05). The OEO groups showed less shift (62.7% similarity coefficient) between these 2 compartments versus the control groups (53.7% similarity coefficient). A reduction of 59% in mortality from ascites was seen in additive-supplemented groups compared with the control group. This study represents the first work to evaluate the effects of the 3 main chemotypes of Origanum genus in broilers.

  19. Contrasting reproductive strategies in three deep-sea octocorals from eastern Canada: Primnoa resedaeformis, Keratoisis ornata, and Anthomastus grandiflorus (United States)

    Mercier, A.; Hamel, J.-F.


    Various aspects of reproduction were studied in three deep-sea octocorals belonging to the order Alcyonacea that co-occur at bathyal depths on the continental edge and the slope of eastern Canada. The main goals were to expand knowledge of deep-water heterotrophic corals and ascertain whether reproductive strategies could explain the known patterns of occurrence. Anthomastus grandiflorus is a gonochoric species with a female-biased sex ratio that exhibits internal fertilization and brooding of planula larvae. Conversely, Primnoa resedaeformis and Keratoisis ornata rely on broadcast spawning and external fertilization; their sexuality remains undetermined as spermatocysts were not found. In P. resedaeformis, the presence of mixed size classes of oocytes in samples from all months, depths, and locations studied suggests continuous oogenesis or overlapping development of oocyte cohorts, indicative of a gametogenic cycle spanning more than a year. No evidence of periodicity was found in this species, although it could have been masked by the striking bathymetric variation in potential relative fecundity (oocytes polyp-1). The two other octocorals displayed a clear annual breeding pattern. Spawning in K. ornata and larval release in A. grandiflorus occurred in late summer and fall, respectively, possibly in response to environmental factors, as supported by shifts in the reproductive peak of A. grandiflorus across latitudes. The three species are presumed to share a nonfeeding larval mode, and data on their reproductive potential do not present any striking disparities. Published data on bycatches and video surveys in Atlantic Canada indicate that the gonochoric brooder A. grandiflorus is more widely distributed than the two free spawners, P. resedaeformis and K. ornata, which is contrary to common dispersal potential paradigms.

  20. Arginine requirement of starting broiler chicks. (United States)

    Cuca, M; Jensen, L S


    Three experiments were conducted to estimate the arginine requirement of male broiler chicks from 0 to 3 wk of age. The experiments were conducted in battery brooders with wires floors, and the birds received water and feed ad libitum. In the first experiment, chicks were fed a diet based on corn, soybean meal, casein, and corn-gluten meal containing 3,200 kcal ME per kg and either 20 or 23% crude protein. Regression analysis indicated an arginine requirement of 1.22% for maximum growth rate and feed efficiency with the 20% protein diet. For chicks fed the 23% protein diet, neither growth rate nor feed efficiency was significantly different among the diets containing arginine ranging from 1.13 to 1.43%. In the second experiment, a basal diet was used containing 17.5% casein and 22.5% protein with arginine ranging from 1.03 to 1.43%. An arginine requirement of 1.18% for maximum body weight gain was estimated by regression analysis, but no significant response to arginine above the basal level was observed for feed efficiency. Performance of chicks fed the basal diet was somewhat reduced because of a difficulty with adherence of feed to the beaks. In a third experiment, three basal diets containing 21, 22, or 23% protein were formulated from practical ingredients without use of casein. The requirement for maximum growth rate and feed efficiency was estimated to be 1.24 to 1.28% for the three diets. The results of these investigations indicate that the arginine requirement for starting chicks suggested by the National Research Council in 1984 of 1.44% in diets containing 3,200 kcal ME per kg is too high for practical diets. The data presented here support an arginine requirement of 1.25%.

  1. Effect of Embryonic Thermal Exposure on Heat Shock Proteins (HSPs) Gene Expression and Serum T3 Concentration in Two Broiler Populations. (United States)

    Rajkumar, U; Vinoth, A; Shanmugam, M; Rajaravindra, K S; Rama Rao, S V


    The present experiment was conducted to evaluate the Hsp-70, 27 and Ubiquitin mRNA expressions and serum T3 concentration in synthetic colored broiler female lines, Punjab Broiler-2 (PB-2), and Naked neck (NN) broiler chicken whose eggs were exposed to 2°C increased incubation temperature for 3 hours each on the 16th, 17 th, and 18th day of incubation. Another set of eggs were incubated at normal conditions that were utilized as the control. A total of 432 chicks, 216 from each breed (PB-2; NN) and treatment (Heat exposed: HE; normal: N), were randomly distributed and reared at high ambient temperatures (32°C-45°C) during the summer season in battery brooders. Birds were sacrificed at 0 and the 28th day post hatch and different tissues (heart, liver, muscle, spleen, and bursa) were collected to study Hsps and ubiquitin mRNA expression. There was no difference between the breeds and age of slaughter in Hsp-70 mRNA expression. The Hsp(70, 27, and ubiquitin) mRNA expression was significantly (P≤0.001) lower in HE birds than that of N birds in PB-2 chickens. Nonsignificant variation was observed in NN chicken. The Hsp-70 mRNA expression was highest in bursa and lowest in muscle and liver. Serum T3 concentration was similar in both HE and N birds. The study concludes that exposure to increased temperature during incubation results in reduced expressions of Hsp mRNA in almost all tissues indicating better thermotolerance of the HE birds.

  2. Monogeneans of West African cichlid fish: evolution and cophylogenetic interactions.

    Directory of Open Access Journals (Sweden)

    Monika Mendlová

    Full Text Available The goals of this paper were to investigate phylogenetic and evolutionary patterns of cichlid fish from West Africa and their Cichlidogyrus and Scutogyrus monogenean parasites, to uncover the presence of host-parasite cospeciation and to assess the level of morphological adaptation in parasites. This required the following steps, each one representing specific objectives of this paper: (1 to build phylogenetic trees for Cichlidogyrus and Scutogyrus species based on ribosomal DNA sequences, (2 to investigate phylogenetic relationships within West African cichlid fish based on the analysis of mitochondrial cytochrome b DNA sequences, (3 to investigate host-parasite cophylogenetic history to gain clues on parasite speciation process, and (4 to investigate the link between the morphology of the attachment apparatus and parasite phylogeny. Phylogenetic analyses supported the monophyletic origin of the Cichlidogyrus/Scutogyrus group, and suggested that Cichlidogyrus is polyphyletic and that Scutogyrus is monophyletic. The phylogeny of Cichlidae supported the separation of mouthbrooders and substrate-brooders and is consistent with the hypothesis that the mouthbrooding behavior of Oreochromis and Sarotherodon evolved from substrate-brooding behavior. The mapping of morphological characters of the haptor onto the parasite phylogenetic tree suggests that the attachment organ has evolved from a very simple form to a more complex one. The cophylogenetic analyses indicated a significant fit between trees using distance-based tests, but no significant cospeciation signal using tree-based tests, suggesting the presence of parasite duplications and host switches on related host species. This shed some light on the diversification process of Cichlidogyrus species parasitizing West African cichlids.

  3. Breeding and mass scale rearing of clownfish Amphiprion percula: feeding and rearing in brackishwater

    Institute of Scientific and Technical Information of China (English)

    DHANEESH Kottila Veettil; AJITH KUMAR Thipramalai Thankappan; SWAGAT Ghosh; BALASUBRAMANIAN Thangavel


    Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater.We designed an indoor program of A.percula culture in brackishwater with a salinity of 24±1,during which the impacts of feed type,water temperature,and light intensity,on the efficiency of its reproduction,were revealed.The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency.Higher reproductive efficiency [number of eggs laid (276±22.3 eggs)] was observed when fish were fed live Acetes sp.rather than clam (204±16.4eggs),trash fish (155±12 eggs) and formulated feed (110±10 eggs).The spawning rate was increased during September and October (water temperature,28.74±0.55℃) on average of 2.4 spawning per month; and low spawning rate was in January (water temperature,24.55±0.45℃) on average of 1 spawning per month.Among three light intensities (100,500,and 900 Ix) set to evaluate larval survival rate,larvae showed the highest survival rate (65.5%) at 900 Ix.The breeding method specifically in brackishwater developed in the present study is a new approach,will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.

  4. White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii. (United States)

    Sahul Hameed, A S; Bonami, Jean-Robert


    Macrobrachium rosenbergii is the most important cultured freshwater prawn in the world and it is now farmed on a large scale in many countries. Generally, freshwater prawn is considered to be tolerant to diseases but a disease of viral origin is responsible for severe mortalities in larval, post-larval and juvenile stages of prawn. This viral infection namely white tail disease (WTD) was reported in the island of Guadeloupe in 1995 and later in Martinique (FrenchWest Indies) in Taiwan, the People's Republic of China, India, Thailand, Australia and Malaysia. Two viruses, Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus-like particle (XSV) have been identified as causative agents of WTD. MrNV is a small icosahedral non-enveloped particle, 26-27 nm in diameter, identified in the cytoplasm of connective cells. XSV is also an icosahedral virus and 15 nm in diameter. Clinical signs observed in the infected animals include lethargy, opaqueness of the abdominal muscle, degeneration of the telson and uropods, and up to 100 % within 4 days. The available diagnostic methods to detect WTD include RT-PCR, dot-blot hybridization, in situ hybridization and ELISA. In experimental infection, these viruses caused 100 % mortality in post-larvae but failed to cause mortality in adult prawns. The reported hosts for these viruses include marine shrimp, Artemia and aquatic insects. Experiments were carried out to determine the possibility of vertical transmission of MrNV and XSV in M. rosenbergii. The results indicate that WTD may be transferred from infected brooders to their offspring during spawning. Replication of MrNV and XSV was investigated in apparently healthy C6/36 Aedes albopictus and SSN-1 cell lines. The results revealed that C6/36 and SSN-1cells were susceptible to these viruses. No work has been carried out on control and prevention of WTD and dsRNA against protein B2 produced RNAi that was able to functionally prevent and reduce mortality in WTD

  5. Turkey knockdown in successive flocks. (United States)

    Evans, R D; Edson, R K; Watkins, K L; Robertson, J L; Meldrum, J B; Novilla, M N


    Turkey knockdown was diagnosed in three of five flocks of hen turkeys on a single farm within a 12-mo period. The age of birds in the flocks affected ranged from 6 wk 2 days to 7 wk 4 days. The attack rate ranged from 0.02% to 0.30% with a case fatality rate in affected birds ranging from 0 to 74%. The diagnosis was made on the basis of clinical signs and histopathologic lesions associated with knockdown. The feed in all flocks contained bacitracin methylene disalicylate and monensin (Coban). Affected birds were recumbent, demonstrated paresis, and were unable to vocalize. Postmortem examination revealed few significant lesions although pallor of the adductor muscles and petechiation in adductor and gastrocnemius muscles were noted. Birds that had been recumbent for extended periods were severely dehydrated. Consistent microscopic lesions included degeneration, necrosis, and regeneration of adductor, gastrocnemius, and abdominal muscles. No lesion in cardiac tissue was noted. Results of our investigation indicated that changes in water consumption, vitamin E status, and brooder to finisher movement correlated with the occurrence of knockdown. Turkey knockdown was defined in 1993 as any condition identified in a turkey flock that has affected the neuromuscular system to a degree that a turkey is unable to walk or stand. This definition was later modified to...neuromuscular or skeletal systems to a degree that a turkey is unable to walk or stand properly. Knockdown may be associated with numerous feed, management, or disease factors alone or in combination. Dosage of monensin, feed restriction/gorging, water restriction, heat stress, copper, mycotoxins, sodium chloride in feed, and sulfa drugs have all been suggested as contributing factors; however, laboratory studies to duplicate this have not been successful. This report presents observations from a single farm at which three of five hen flocks in a single year experienced knockdown. When a flock was reported as

  6. Induced reproduction and reproductive characteristics of Rhinelepis aspera Agassiz, 1829 (osteichthyes: Siluriformes, Loricariidae

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    Yoshimi Sato


    Full Text Available Rhinelepis aspera is the largest Loricariidae species found in the São Francisco river basin where it is now rarely caught. Brooders kept in tanks were hypophysed with crude carp pituitary extract (CCPE. Approximately 82% of the females responded positive to the treatment. The egg was opaque, demersal, round, yellow and adhesive. Egg stripping was done at 212 hour-degrees (= 8.2 h after application of the second dose of CCPE (water temperature = 25-26 ºC. Hatching of the larvae occurred at 1022 hour-degrees (= 42.2 h, after fertilization of the eggs (water temperature =24-25 ºC. Fertilization rate of the eggs was 72%. Absolute fecundity (AF, initial fertility (IF and final fertility (FF in relation to the females' body weight are expressed, respectively, by the equations: AF = - 33993 + 122308 Wt (r² = 0.88, IF = - 14823 + 58619 Wt (r² = 0.71 and FF = - 6553 + 29741 (r² = 0.61Rhinelepis aspera é a espécie que atinge maior porte dentre os Locariidae da bacia do rio São Francisco onde é hoje raramente capturada. Reprodutores mantidos em viveiros foram hipofisados com extrato bruto de hipófise de carpa (EBHC. Aproximadamente 82% das fêmeas responderam positivamente ao tratamento. Seus ovos eram opacos, demersais, arredondados, amarelados e adesivos. A extrusão dos ovos foi feita a 212 horas-graus (= 8,2 h após a aplicação da segunda dose de EBHC (temperatura da água = 25-26 ºC. A eclosão das larvas ocorreu a 1022 horas-graus (= 42,2 h após a fertilização dos ovos (temperatura da água = 24-25 ºC. A taxa de fertilização dos ovos foi de 72%. Fecundidade absoluta (AF, fertilidade inicial (IF e fertilidade final (FF em relação ao peso corporal das fêmeas foram expressa, respectivamente, pelas equações: AF = - 33993 + 122308 Wt (r² = 0,88, IF = - 14823 + 58619 Wt (r² = 0,71 e FF = - 6553 + 29741 (r² = 0.61.

  7. Does encapsulation protect embryos from the effects of ocean acidification? The example of Crepidula fornicata.

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    Fanny Noisette

    Full Text Available Early life history stages of marine organisms are generally thought to be more sensitive to environmental stress than adults. Although most marine invertebrates are broadcast spawners, some species are brooders and/or protect their embryos in egg or capsules. Brooding and encapsulation strategies are typically assumed to confer greater safety and protection to embryos, although little is known about the physico-chemical conditions within egg capsules. In the context of ocean acidification, the protective role of encapsulation remains to be investigated. To address this issue, we conducted experiments on the gastropod Crepidula fornicata. This species broods its embryos within capsules located under the female and veliger larvae are released directly into the water column. C. fornicata adults were reared at the current level of CO2 partial pressure (pCO2 (390 μatm and at elevated levels (750 and 1400 μatm before and after fertilization and until larval release, such that larval development occurred entirely at a given pCO2. The pCO2 effects on shell morphology, the frequency of abnormalities and mineralization level were investigated on released larvae. Shell length decreased by 6% and shell surface area by 11% at elevated pCO2 (1400 μatm. The percentage of abnormalities was 1.5- to 4-fold higher at 750 μatm and 1400 μatm pCO2, respectively, than at 390 μatm. The intensity of birefringence, used as a proxy for the mineralization level of the larval shell, also decreased with increasing pCO2. These negative results are likely explained by increased intracapsular acidosis due to elevated pCO2 in extracapsular seawater. The encapsulation of C. fornicata embryos did not protect them against the deleterious effects of a predicted pCO2 increase. Nevertheless, C. fornicata larvae seemed less affected than other mollusk species. Further studies are needed to identify the critical points of the life cycle in this species in light of future ocean


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    Fisseha Moges


    Full Text Available Broiler introduction and adaptation trial was done at Bahir Dar city in the year 2011/2012. The major objective of the trial was to introduce broiler breeds in to the region and evaluate their performance at smallholder level. A total of 1100 day old chicks of Hubbard Classical breed were purchased from Debre Zeit Agricultural Research Center and transported to Bahir Dar. Each participant received, on average, 109 day old chicks and 400kg started and finisher feed. Brooding was done using electrical brooder. Finished broilers were sold live and in processed form after six weeks of age. The average weight old day old chicks was 45.5g. The average weight of birds at end of 1st, 2nd, 3rd, 4th, 5th and 6th weeks were 136g, 364g, 711g, 1174g, 1665g and 2092g, respectively. The average final weight was 2092g (ranged 1957g - 2216g. The average cumulative mortality was 4.8% (Ranged 1.9%-6.5%. The average daily feed intake and cumulative feed intake of each bird during the entire period was 99g and 4052g, respectively. The average FCR was 1.84. The partial budget analysis result indicated that broiler production was profitable with a net benefit of 10.75 Eth Birr/head and 19.3 Ethi0pian Birr/kg, respectively. The result showed that participants who sold processed broiler meat fetched 8.6 Eth Birr/kg more than those participants who sold live birds. The survey result indicated that all participants were highly satisfied by the breed. According to the producers; the breed has paramount merit than other chicken breeds like; very fast growth, high meat production and short rearing period. The higher final body weight, the lower mortality, higher profitability and higher market demand revealed that broilers could be reared at small holder level in big cities like Bahir Dar. The result of the trial showed that actions should be taken to solve the prevailing constraint including; lack of day old chicks and quality feed. One of the main lessons we learned from

  9. Aspectos reproductivos del acocil Cambarellus (Cambarellus montezumae (Crustacea: Decápoda: Cambaridae en condiciones controladas Breeding aspects of the crayfish Cambarellus (Cambarellus montezumae (Crustacea: Decapoda: Cambaridae under controlled conditions

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    José Luis Arredondo-Figueroa


    Full Text Available El acocil Cambarellus (C. montezumae es una especie endémica cuya distribución está restringida a algunos cuerpos de agua lacustre. Se presentan los resultados sobre aspectos reproductivos de esta especie en condiciones controladas, con el objetivo de determinar la factibilidad de la producción de huevos y juveniles y su potencial aplicación a programas de rescate, repoblación y cultivo. Se realizaron 2 ensayos experimentales, el primero en 4 estanques exteriores de 800 litros conectados a sistemas cerrados de recirculación (SCR y el segundo en recipientes de plástico en laboratorio (RPL, de 2 litros. Para el primer ensayo, se manejaron 219 reproductores durante 335 días, obteniendo 136 hembras ovígeras. Cada hembra produjo en promedio 45 ± 23 huevos por puesta. El 83.7% de los huevos eclosionaron, obteniéndose un total de 3 162 juveniles. En los RPL se manejó una población F1 obtenida a partir de los reproductores del SCR en una relación 1 hembra: 2 machos, con 10 repeticiones. La producción de huevo fue variable con un promedio de 34 por hembra. Los indicadores de desempeño mostraron diferencias significativas (P ≤ 0.05 entre sexos con respecto a 6 de ellos. Los resultados indican la factibilidad para su cultivo y producción de huevos y juveniles.Cambarellus (C. montezumae is an endemic crayfish from Mexico, which actual distribution is restricted to few lacustrine bodies of water. Here we present results on the reproduction of this species under controlled conditions with the aim to determine the feasibility of the production of eggs and juveniles, as an strategy for their rescue, re-population and culture. Two experimental assays were conducted, the first one, in 4 outdoor 800-L tanks recirculation aquaculture systems (RAS and the second, in 2-L plastic containers under laboratory conditions (PCL. For the first assay, 219 brooders were used during 335 days , obtaining 136 ovigerous females. Each female produced an average

  10. Sex-associated variations in coral skeletal oxygen and carbon isotopic composition of Porites panamensis in the southern Gulf of California (United States)

    Cabral-Tena, Rafael A.; Sánchez, Alberto; Reyes-Bonilla, Héctor; Ruvalcaba-Díaz, Angel H.; Balart, Eduardo F.


    Coral δ18O variations are used as a proxy for changes in sea surface temperature (SST) and seawater isotope composition. Skeletal δ13C of coral is frequently used as a proxy for solar radiation because most of its variability is controlled by an interrelationship between three processes: photosynthesis, respiration, and feeding. Coral growth rate is known to influence the δ18O and δ13C isotope record to a lesser extent than environmental variables. Recent published data show differences in growth parameters between female and male coral in the gonochoric brooding coral Porites panamensis; thus, skeletal δ18O and δ13C are hypothesized to be different in each sex. To test this, this study describes changes in the skeletal δ18O and δ13C record of four female and six male Porites panamensis coral collected in Bahía de La Paz, Mexico, whose growth bands spanned 12 years. The isotopic data were compared to SST, precipitation, photosynthetically active radiation (PAR), chlorophyll a, and skeletal growth parameters. Porites panamensis is a known gonochoric brooder whose growth parameters are different in females and males. Splitting the data by sexes explained 81 and 93 % of the differences of δ18O, and of δ13C, respectively, in the isotope record between colonies. Both isotope records were different between sexes. δ18O was higher in female colonies than in male colonies, with a 0.31 ‰ difference; δ13C was lower in female colonies, with a 0.28 ‰ difference. A difference in the skeletal δ18O could introduce an error in SST estimates of ≈ 1.0 to ≈ 2.6 °C. The δ18O records showed a seasonal pattern that corresponded to SST, with low correlation coefficients (-0.45, -0.32), and gentle slopes (0.09, 0.10 ‰ °C-1) of the δ18O-SST relation. Seasonal variation in coral δ18O represents only 52.37 and 35.66 % of the SST cycle; 29.72 and 38.53 % can be attributed to δ18O variability in seawater. δ13C data did not correlate with any of the environmental

  11. Dietas para frangos de corte contendo quirera de arroz Performance of broiler feed with broken rice in diets

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    Berilo de Souza Brum Júnior


    Full Text Available O experimento foi realizado para avaliar o uso da quirera de arroz na dieta de frangos de corte. Foram utilizados 240 pintos machos, alojados em galpão, contendo 12 boxes de 2m² de área. Cada box continha comedouro tubular, bebedouro pendular e campânula nos 21 dias iniciais. As aves foram distribuídas em um delineamento inteiramente casualizado, com três tratamentos e quatro repetições, as quais receberam dietas contendo 0, 20 e 40% de inclusão de quirera de arroz. Os dados foram submetidos à análise de regressão e à análise de variância e, quando houve diferença, aplicou-se o teste de Tukey. O ganho de peso, o consumo de ração e a conversão alimentar de 1-21, 1-35 e 1-42 dias (P>0,05 não foram afetados pela substituição do milho por quirera de arroz. A umidade de cama, o índice de eficiência produtivo, o rendimento de carcaça quente, de fígado, coração, coxa, sobre-coxa e peito não apresentaram diferença (P>0,05. No entanto, os rendimentos de moela, bem como a pigmentação da canela e do bico, diminuíram linearmente com o aumento do nível de quirera de arroz na dieta. Conclui-se que a quirera de arroz pode ser incluída nas dieta, substituindo o milho, para frangos de corte.An experiment was carried to evaluate the performance of broilers fed with diferent levels of broken rice. The experiment was conducted with 240 males broilers chicks, Cobb 500, were distributed in floor pens of 2m² each, with one tubular feeder, one pendular drinker, an electrical brooder and on the top of rice hulls. An entirely randomized experimental design was used, with 3 treatments and 4 replicates with 20 birds each, which received, diets containing 0, 20 and 40% of broken rice. Data were submitted to Analisis of regression. Weight gain, feed intake, feed conversion to the1- 21, 1- 35 and 1-42 days of age, litter humidity, carcass, liver, breast, thigh and drumstick yield did not present significant difference. The gizzard and the

  12. Reproduction in cold water: paradigm changes in the 20th century and a role for cidaroid sea urchins (United States)

    Pearse, John S.; Lockhart, Susanne J.


    rather than feeding (planktotrophic) larvae, and these, along with brooding species, generally do reproduce throughout the year. Yet brooding is almost certainly not an adaptation to low temperatures and low larval food supply, as supposed earlier; instead, the species-rich clades of brooders probably reflect enhanced speciation under unique conditions in the Antarctic. In addition, despite recent evidence of constraints on metabolic flexibility, low metabolic rates may themselves be adaptations to Antarctic conditions. Just as a growing body of "exceptions" to the early 20th century paradigms led to their breakdown, these persisting exceptions to current ideas demand that our existing paradigms be re-examined for further insight into the biology of marine animals in cold water. Cidaroid echinoids, in particular, appear to support some of the earlier ideas. The group consists of one or possibly two clades that make up over 80% of the species of regular sea urchins in the Antarctic; several species extend into the deep sea. One putative species is nearly bipolar, extending from the Antarctic shelf into the deep sea and then north in the eastern Pacific to Alaska. All the species appear to brood embryos, but development in the brood might be facultative. Reproduction seems to be aseasonal, albeit infrequent. Growth rates are probably very slow and longevity very long. Current work begun with ANDEEP is directed at resolving the phylogenetic history of this group to better understand unusual reproductive and other features in cold-water marine animals.

  13. Acondicionamiento de reproductores, desove y cultivo larval de Graus nigra (Philippi, 1887 (Kyphosidae: Girellinae Broodstock conditioning, spawning and larval culture of Graus nigra (Philippi, 1887 (Kyphosidae: Girellinae

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    Avelino Muñoz


    Full Text Available Se describen resultados sobre acondicionamiento reproductivo, desove y cultivo larval de Graus nigra ("vieja negra", "mulata". Peces adultos silvestres se recolectaron y se utilizaron como reproductores, los que al final del período de acondicionamiento alcanzaron el estado de maduración gonadal y desovaron en forma natural y espontánea. Los huevos fueron recolectados y después de 36 h de incubación eclosionaron, con una tasa de eclosión promedio de 60%. Las larvas recién eclosionadas midieron 2,9 ± 0,23 mm y alcanzaron el día 50 post-eclosión (PE una longitud total de 12,6 ± 0,37 mm. La sobrevivencia larval posterior a la eclosión fue entre 50,9 y 79,1% y al día 30 PE fue de 12,1%. El cultivo larval se desarrolló en estanques con suministro de agua de mar filtrada y esterilizada. Después de la reabsorción del saco vitelino se produjo el desarrollo del tracto digestivo y las larvas se alimentaron con dieta viva enriquecida con emulsión de ácidos grasos altamente insaturados. A los 35 días de cultivo se ofreció alimento artificial a las larvas cuyo tamano fue aumentando progresivamente a medida que progresó su desarrollo ontogénico. Se describe la evolución anatómica de las larvas y las relaciones morfométricas que representan su desarrollo; se caracteriza el patrón de crecimiento de las larvas hasta los 50 días post-eclosión y se discuten aspectos relacionados con la sobrevivencia larval y la introducción de mejoras para optimizar la producción de larvas y juveniles.In this study results related to reproductive conditioning, spawning and larval culture of Graus nigra ("vieja negra", "mulata" are given. Wild adult fishes were collected and used as brooders which at the end of the conditioning period reached gonadal maturation state and spawned naturally and spontaneously. Eggs were collected and after 36 hours of incubation they hatched at average rate of 60%. The hatched larvae measured 2.9 ± 0.23 mm and at day 50

  14. Asociación del color de la concha de reproductores de Argopecten purpuratus (Lamarck, 1819 con la supervivencia, crecimiento y desarrollo larval de sus progenies Association between shell color of breeds (Lamarck, 1819 and the survival, growth and larval development of their progenies

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    Ricardo M García


    most frequent shell colors. Knowing that Argopecten purpuratus shell color variation is under genetic control, in this work we test the hypothesis that loci that control shell color variation also affect larvae's growth rate, survival and/or development rate. Survival, growth and development rates were estimated in larvae produced in different crosses between brooders of A. purpuratus having low frequency shell colors (white or orange and "normal" (brown color. Results showed no significant differences in growth rates between larvae produced by crosses that involved brown shell parents, or orange by white parents. However, progenies of self fertilized orange and white parents showed significant differences in growth among them, and a lower growth rate than the remaining crosses. Results suggest that genes that control shell color variation in juvenile and adult A. purpuratus could affect the growth rates of their larvae, but not the development rate or survival.