Anja B. Riber; Guzman, Diego A.
Simple Summary Chicks require heat to maintain body temperature during the first weeks after hatch. Heat is normally provided by use of heating lamps or whole-house heating, but an alternative is dark brooders, i.e. horizontal heating elements equipped with curtains. The effects of providing layer chicks with dark brooders during the brooding period on behavior and fearfulness were investigated. Brooders resulted in chicks showing less locomotive activity, feather pecking and fleeing. Also, a...
Riber, Anja B.; Guzman, Diego A.
Simple Summary Chicks require heat to maintain body temperature during the first weeks after hatch. Heat is normally provided by use of heating lamps or whole-house heating, but an alternative is dark brooders, i.e. horizontal heating elements equipped with curtains. The effects of providing layer chicks with dark brooders during the brooding period on behavior and fearfulness were investigated. Brooders resulted in chicks showing less locomotive activity, feather pecking and fleeing. Also, a long-term reduction of fearfulness in brooder birds was found. Results support the suggestion that rearing with dark brooders can be a successful method of reducing or preventing some of the major welfare problems in layers. Abstract Chicks require heat to maintain body temperature during the first weeks after hatch. This may be provided by dark brooders; i.e., horizontal heating elements equipped with curtains. The objective was to test effects of rearing layer chicks with dark brooders on time budget and fearfulness. Behavioral observations were performed during the first six weeks of age. Three different fear tests were conducted when the birds were age 3–6, 14–15 and 26–28 weeks. During the first four days, brooder chicks rested more than control chicks whereas they spent less time drinking, feather pecking and on locomotion (p ≤ 0.009). On days 16, 23, 30 and 42, brooder chicks spent less time on feather pecking, locomotion and fleeing (p ≤ 0.01) whereas foraging and dust bathing occurred more often on day 42 (p ≤ 0.032). Brooder birds had shorter durations of tonic immobility at all ages (p = 0.0032), moved closer to the novel object at age 15 weeks (p < 0.0001), and had shorter latencies to initiate locomotion in the open-field test at age 28 weeks (p < 0.0001). Results support the suggestion that dark brooders can be a successful method of reducing or preventing fear and feather pecking in layers. PMID:26751482
Riber, Anja Brinch; Guzman, Diego A.
. Behavioral observations were performed during the first six weeks of age. Three different fear tests were conducted when the birds were age 3–6, 14–15 and 26–28 weeks. During the first four days, brooder chicks rested more than control chicks whereas they spent less time drinking, feather pecking and on...
Isolamento de enterobactérias em Alphitobius diaperinus e na cama de aviários no oeste do estado do Paraná, Brasil Isolation of enteric and litter organisms from Alphitobius diaperinus in brooder chickens houses in west of Parana State, Brazil
Full Text Available O objetivo deste trabalho foi realizar o isolamento de bactérias da família Enterobacteriaceae em adultos de A. diaperinus, buscando uma correlação entre as bactérias presentes no inseto e na cama, em aviários para produção de frangos de corte no oeste do Paraná, Brasil. No primeiro experimento, insetos adultos foram coletados em 14 granjas. No segundo experimento, foram coletados insetos e material da cama de 12 diferentes aviários. Os adultos foram anestesiados com éter, macerados em solução salina e o material da cama colhido por "swab" de arrasto. O enriquecimento não seletivo foi feito com caldo BHI e o seletivo com Rappaport-Vassiliadis e Tetrationato. Os meios de cultivo para plaqueamento foram o ágar MacConkey, ágar Salmonella-Shigella e ágar verde-brilhante. As enterobactérias isoladas em adultos de A. diaperinus foram: Proteus Vulgaris, P. mirabilis, Escherichia Coli, Enterobacter spp., E. agglomerans, E. gergoviae, E. sakasakii, Citrobacter Diversus e Klebsiella Pneumoniae, enquanto que na cama foram encontrados Proteus Vulgaris, P. mirabilis, Escherichia Coli, Enterobacter Agglomerans. Não foram isoladas Salmonella spp. do inseto nem da cama e P. vulgaris foi a predominante. E. coli foi freqüente nas granjas, tanto na cama como nos insetos e contribuem na disseminação da colibacilose em aviários.This study was conducted to determine bacterial species of Enterobacteriaceae in adults of lesser mealworm, A. diaperinus and make the correlation of the bacterial species in the insects and in the litter that infest poultry brooder houses in West of Parana State, Brazil. In the first experiment, the adults were collected in 14 poultry houses. In the second experiment the insects and the litter were collected in 12 poultry houses. The adults were anaesthetized with eter, macerated in sterile saline solution and the litter material was taken off by swab. The enrichment broth was plated on the BHI and in Rappaport
LIU Xiande; SUI Banliang; WANG Zhiyong; CAI Mingyi; YAO Cuiluan; CHEN Qingkai
We estimated the reproductive success of adult brood fish and the heritability of growthrelated traits for large yellow croaker (Larimichthys crocea).We created two independent full-factorial cross groups (1 and 2) by crossing 4 males×4 females and 4 males×3 females,respectively.We measured the body weight (BW),body length (BL),and body height (BH) of 281 individuals from group 1 and 318 individuals from group 2 at 20 months post hatch (harvest age).We also collected a tissue sample from each individual.The parents and offspring were genotyped using six polymorphic microsatellites.Of the 599 offspring,99.2％ were assigned to a single pair of parents.In both groups,some parent pairs produced a large number of offsprings while other pairs did not produce any offspring.The genetic diversity and putative Ne were lower in the offsprings than in the parents in both groups.The heritability estimates at 20-month age were 0.13±0.10 for BW,0.19±0.13 for BL,and 0.09±0.06 for BH.The genetic and phenotype correlation between BW,BL,and BH was close to 1.The results provide basic information for selective breeding and further genetic characterization of large yellow croaker.
Full Text Available During the breeding season, lots of physiological changes occur in the bodies of fish which lead to other phenotypical, anatomical and behavioral changes. To evaluate the impact of breeding on the bodily composition of fish, tests were conducted to determine the fatty acid profile of the brooder and Non-brooder wild and farmed fish. Samples were analyzed by gas liquid chromatography using a flame ionization detector.There are non significant differences in the amount of saturated and unsaturated fatty acids in wild and farmed nonbrooder fish whereas in case of brooder there are significant differences. Brooder farmed fish contained more saturates in gonads approximately twice that of wild fish with C16:0 as the dominating fatty acid. C18:2n-6 and C20:5n-6 were the dominating unsaturated fatty acids in the gonads. Non-brooder fish contained more unsaturated fatty acids in the meat as compared to the brooder fish and farmed fish contained higher amounts of saturated fatty acids as compared to wild fish. C18:1n-9 and C20:4n-6 fatty acids were found in higher quantities and among the saturated acids, C16:0 is the dominating one. More or less similar results were found in the case of the liver fatty acid profile of breeding and nonbreeding fish. Fish of lower weight categories contained more unsaturated fatty acids and so better for consumption to leave the brooders of higher weight categories for the breeding of future generation.Durante la época de reproducción numerosos cambios fisiológicos ocurren en el cuerpo de los peces que guían a cambios anatómicos y de comportamiento. Para evaluar el impacto de la etapa de reproducción en la composición de los peces, ensayos para determinar el perfil de ácidos grasos fueron conducidos en peces salvajes y criados, en dos categorías de peso, maduro (booder o inmaduro (non-brooder. Las muestras fueron analizadas por cromatografía de gases usando un detector de ionización de llama. No hubo
Fleming, W.J.; Heinz, G.H.; Franson, J.C.; Rattner, B.A.
Diets mixed to contain 0,0.1, 1.0, 10 and 100 ppm temephos (determined chemically to contain less than 0.5, less than 0.5, 0.89, 6..0 and 59 ppm temephos, respectively) in an Abate ? 4E formulation, were fed to mallard (Anas platyrhynchos) ducklings for 7 d. During this period, half of the ducklings in each dietary treatment group were housed in a heated brooder (39 to 41?C) and half were housed in an unheated brooder (10 to 18?C). Mortality in all dietary groups in the unheated brooder was higher than in the heated brooder. High temephos-related mortality occurred in the 100 ppm group in the unheated brooder but not in any other diet-temperature groups. Ingestion of the 100 ppm temephos diet inhibited plasma cholinesterase (ChE) activity and elevated plasma corticosterone concentration and creatine phosphokinase activity, but other selected plasma chemistries were not affected in a dose-related manner. Brain ChE activity was depressed only in the 100 ppm dietary groups; maximum inhibition of brain ChE activity was 48%. These findings suggest that diets containing up to 10 ppm temephos do not directly affect duckling survival during the first week of life and that the toxicity of 100 ppm temephos is markedly enhanced by cold.
Hertel, Paula T; Benbow, Amanda A; Geraerts, Elke
Ruminative habits of thought about one's problems and the resulting consequences are correlated with symptoms of depression and cognitive biases (Nolen-Hoeksema, Wisco, & Lyubomirsky, 2008). In our orienting task, brooders and non-brooders concentrated on self-focusing phrases while they were also exposed to neutral target words. On each trial in the unfocused condition, participants saw and then reported the target before concentrating on the phrase; in the focused condition, the target was reported after phrase concentration. A brooding-related deficit on a subsequent unexpected test of free and forced recall was obtained in the unfocused condition only. Brooders recalled more successfully in the focused than in the unfocused condition. Thus, impaired recall of material unrelated to self-concerns may be corrected in situations that constrain attention. PMID:22671938
Full Text Available In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males × 10 females of Asian seabass to reconstruct pedigree and monitor the change of genetic variations. In all four crosses, over 96.8% of the offspring could be assigned to their parents, indicating the high power of the nine microsatellites for parentage assignment. This study revealed several interesting results: (1. In all four crosses, the contribution of parents to offspring was significantly uneven, and some dominant breeding fishes (i.e. brooders were found; (2. In two mass crosses where the brooders were carefully checked for reproductive status, a majority (≥ 90% of brooders contributed to offspring, whereas in another two crosses, where the brooders were randomly picked without checking reproductive status, only a few brooders (40.0-45.0% produced offspring; (3. Females had more problems in successful spawning compared to males; and (4. In the two crosses where a few brooders produced offspring, there was a substantial loss in allelic (24.1-34.3% and gene (20.5-25.7% diversities in offspring, while in the other two crosses, the majority of allelic (96.8-97.0% and gene diversities (94.8-97.1% were maintained. These observations suggest that a routine molecular parentage analysis is required to maintain both allelic and gene diversity in breeding Asian seabass.
The US is one of the world’s largest importers of seafood. A major constraint in producing hybrid striped bass is suboptimal production efficiency due to large performance variation of fish from undomesticated brooders. The objectives of this first-year study were to determine the genetic basis of p...
Ochieng, Justus; Owuor, George; Bebe, Bockline Omedo
In Africa, many rural farming households keep indigenous chickens (Gallus domesticus) in traditional scavenging systems characterized by low input and low output. To improve productivity, African governments and development partners disseminate a management intervention package consisting of feed supplementation, vaccination, brooder, chick rearing equipment and improved housing. Some smallholder farmers adopt the full package, while others adopt the feed supplementation and vaccination only,...
@@ Shiyan Dongfeng Innovation,Brooder, Industrial Zone Ⅰ. The Background of The Project and the Enterprise The project is located at Xiejia Val-lage, Checheng Sub-district, Kaixuan Avenue, invested by Xianglong Real Estate Co., Ltd. It covers an area of 82.3 acres.
Saeed Hajirezaee; Bagher M. Amiri; Ali R. Mirvaghefi
Problem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, semen volume and chemical composition of seminal fluid) during these sequential strippings. Approach: The ...
Nizamettin Senkoylu; Hasan Akyurek; Fisun Koc; Aylin Agma Okur; Hasan Ersin Samli; Mehmet Levent Ozduven
The experiment was conducted to evaluate the effects of organic acid mixture and/or mannanoligosaccharides (MOS) on growth performance, blood parameters and intestinal microbiota in 120 Ross 308 male broiler chicks, over a period of 21 days. Birds were maintained in battery brooders confined in an environmentally controlled experimental room. There were 4 dietary treatments, each consisting of 6 replicates. Dietary treatments were: (i) basal diet (as a control), (ii) basal diet + MOS 2 kg/ton...
Zhu, W; Jiang, W; Wu, L Y
The current intensive indoor production system of commercial Pekin ducks never allows adequate water for swimming or wetting. Therefore, heat stress is a key factor affecting health and growth of ducks in the hot regions and season. Experiment 1 was conducted to study whether heat stress was deleterious to certain organs of ducks. Forty-one-day-old mixed-sex Pekin ducks were randomly allocated to four electrically heated battery brooders comprised of 10 ducks each. Ducks were suddenly exposed to 37 °C ambient temperature for 3 h and then slaughtered, in one brooder at 21 days and in another brooder at 49 days of age. The results showed that body weight and weight of immune organs, particularly liver markedly decreased in acute heat stress ducks compared with the control. Experiment 2 was carried out to investigate the influences of dietary L-arginine (Arg) supplement on weight and compositions of certain lymphoid organs, and growth performance in Pekin ducks, under daily cyclic hot temperature environment. A total of 151-day-old mixed-sex Pekin ducks were randomly divided into one negative control and two treatment groups, fed experimental diets supplemented with 0, 5, and 10 g L-Arginine (L-Arg)/kg to the basal diet respectively. Ducks were exposed to cyclic high temperature simulating natural summer season. The results showed that the addition of L-Arg improves feed conversion ratio (FCR) during a period of 7-week trial, as well as increases hepatic weight relative to body weight at 21 days, while decreases the hepatic water content at 49 days of age. This study indicated that the liver was more sensitive to acute heat stress, and the hepatic relative weight and chemical composition could be regulated by dietary L-Arg supplementation in Pekin ducks being reared at high ambient temperature. These beneficial effects of Arg on liver might be a cause of improved FCR. PMID:24773570
B. Indarsih; R.A.E.Pym
The study was conducted as a completely randomized design, with a factorial arrangement to determine the response of commercial broilers to choice feeding and limiting amino acids on growth and carcass performance. A total of 432 male birds were weighed at one-d-old and randomly distributed to 48 wire-floored brooder cage each 1.0 m2. There were 2 sexes and 4 dietary treatments with 6 replicates each of 9 birds. Birds were given one of three dietary regimens with dietary change every 7 days. ...
The response of Cobb and Ingham strain commercial broilers to a range of dietary amino acids in a factorial experiment (2 strains x 2 sexes x 3 dietary treatments) with 3 replicates was evaluated. A total of 180 birds was sexed and weighed at one-day-old and randomly distributed to 36 brooders. Summit (246 g CP and 16.8 g lysine/kg) and dilution (120 g CP and 6.0 g lysine/kg) diets were prepared in isocaloric at 3100 kcal ME/kg. The estimated dietary level of crude protein for all birds at da...
Full Text Available A study was conducted to evaluate the effect of age of release, strain and farmers location on growth and carcass performance of the indigenous, and two exotic strains (Harco® and Goldline® cockerels, reared on free range; in order to ascertain the best age of release into free range from brooder houses, as well as the effect of farmers location and strain on their growth and carcass characteristics, in South Eastern Nigeria. A total of 315 cockerels comprising 87 indigenous chicks, 114 Harco® chicks, and 114 Goldline® cockerels brooded and raised up to 18 weeks of age were used in this experiment. These birds were randomly released to farmers in the villages at three different ages of 4, 8 and 12 weeks to run on free range. There were significant effects of age of release and strain on the growth characteristics, but none on the carcass characteristics. However, there was also significant effect of the zone on both growth and carcass traits measured. It was concluded that the earlier the exotic birds are released from the brooder house, the better their performance on scavenging rearing. This is beneficial as the farmer will save much from the purchase of compounded feed and other veterinary services.
Bruckner, Andrew W; Borneman, Eric H
In June, 2002, the government of Dominica requested assistance in evaluating the coral culture and transplantation activities being undertaken by Oceanographic Institute of Dominica (OID), a coral farm culturing both western Atlantic and Indo-Pacific corals for restoration and commercial sales. We assessed the culture facilities of OID, the condition of reefs, potential impacts of coral collection and benefits of coral transplantation. Coral reefs (9 reefs, 3-20 m depth) were characterized by 35 species of scleractinian corals and a live coral cover of 8-35%. Early colonizing, brooders such as Porites astreoides (14.8% of all corals), P. porites (14.8%), Meandrina meandrites (14.7%) and Agaricia agaricites (9.1%) were the most abundant corals, but colonies were mostly small (mean = 25 cm diameter). Montastraea annularis (complex) was the other dominant taxa (20.8% of all corals) and colonies were larger (mean = 70 cm). Corals (pooled species) were missing an average of 20% of their tissue, with a mean of 1.4% recent mortality. Coral diseases affected 6.4% of all colonies, with the highest prevalence at Cabrits West (11.0%), Douglas Bay (12.2%) and Coconut Outer reef (20.7%). White plague and yellow band disease were causing the greatest loss of tissue, especially among M. annularis (complex), with localized impacts from corallivores, overgrowth by macroalgae, storm damage and sedimentation. While the reefs appeared to be undergoing substantial decline, restoration efforts by OlD were unlikely to promote recovery. No Pacific species were identified at OID restoration sites, yet species chosen for transplantation with highest survival included short-lived brooders (Agaricia and Porites) that were abundant in restoration sites, as well as non-reef builders (Palythoa and Erythropodium) that monopolize substrates and overgrow corals. The species of highest value for restoration (massive broadcast spawners) showed low survivorship and unrestored populations of these
The analysis of physico-chemical and biological parameters of two fish ponds; brooder rearing pond (Pond-I) and fry stocking pond (Pond-II), at Chilya fish hatchery were carried out during January - December 2006. Ranges of temperature of water 14-35.2 degree C, 14.5-34.7 degree C; dissolved oxygen 7.69-12.71 mg/l, 7.82-12.3 mg/l; pH 7.2-7.9, 7.87-8.4; total alkalinity 75-142 mg/l, 106-181 mg/l; total hardness 83-120, 102-189 mg/l; total dissolved solids (TDS), 270-348, 226-3920 mg/l; total nitrogen 0.15-0.47, 0.528-1.47 mg/l and orthophosphate 0.12-0.372, 0.74-1.21 mg/l respectively were recorded at the ponds. Algal flora in Pond-I consisted of 7 species of Cyanophyta, 11 species of Chlorophyta while in Pond-II 12 species of Cyanophyta, 13 species of Chlorophyta and 2 species of Bacillariophyta were found,7 species of aquatic plants, Hydrilla verticillata, Najaz minor, Potamogeton pectinatus, Nymphia sp. Typha domingensis and Phragmites vallatorn were also recorded from pond -II. Among zooplankton the brooder pond had the population of rotifers (5 species) and micro crustaceans (3 species). In fry pond, 4 species of rotifers and 7 species of micro crustaceans were present. The results revealed that the water quality variables of Chilya fish hatchery were suitable for fish culture. No known source of pollution enters the ponds. On the northern side there is an outlet channel of Keenjhar lake which supplies water to fish ponds. Rich algal flora and zooplanktons were observed in Pond-II (fry pond). This may be due to the artificial feeding and availability of macrophytes which maintain the water temperature, light penetration, nitrates, phosphates and total dissolved solids (TDS). (author)
Gang Pan, Jinzeng Yang
Full Text Available Pacific threadfin, Polydactylus sexfilis, is popular fish in recreational fishing, as well as aquaculture in Hawaii. Its natural population has been continuously declining in the past several decades. Microsatellite DNA markers are useful DNA-based tool for monitoring Pacific threadfin populations. In this study, fifteen Microsatellite (MS DNA markers were identified from a partial genomic Pacific threadfin DNA library enriched in CA repeats, and six highly-polymorphic microsatellite loci were employed to analyze genetic similarity and differences between the wild population and hatchery population in Oahu Island. A total of 37 alleles were detected at the six MS loci in the two populations. Statistical analysis of fixation index (FST and analysis of molecular variance (AMOVA showed no genetic differentiation between the wild and hatchery populations (FST=0.001, CI95%= -0.01-0.021. Both high genetic diversity (Ho=0.664-0.674 and He=0.710-0.715 and Hardy-Weinberg equilibrium were observed in the wild and hatchery populations. Results of genetic bottleneck analysis indicated that the hatchery was founded with sufficient numbers of brooders as inbreeding coefficient is very low (FIS=0.052-0.072 in both wild and hatchery populations. Further studies are needed for comprehensive determinations of genetic varieties of primary founder broodstocks and successive offspring of the hatchery and wild populations with increased number of Pacific threadfin sample collections.
Full Text Available Amphiprion sebae kept in the recirculating Marine Research Aquarium spawned a maximum of 0.8 times per month throughout the year. This is the first spawning record from a recirculating aquarium environment. Generally the spawning of A. sebae has been described from fresh seawater by various authors. The present study mainly observes the maintenance of brooders, their pairing and spawning in recirculating aquarium environment. Among the 54 adult fishes collected from the natural environment, three pairs are formed in the aquaria. The parental care of egg guarding male was observed. Three pairs are fed with different feed i.e., prawn meat, fish meat and a combination of prawn, fish and clam meats. The spawning frequency was observed to be moderately high (0.8/month when fed with the mixed feed, whereas a frequency of 0.5 and 0.6 per months was observed when fed with prawn, fish meats respectively. The maximum clutch size (911 was observed also in mixed feeds and it was minimum (395 when the pairs were fed with fish meat alone. The increase in the frequency of spawning was during summer months compared to other months.
Helena Phoenix Baird
Full Text Available Currently there is very limited understanding of genetic population structure in the Antarctic benthos. We conducted one of the first studies of microsatellite variation in an Antarctic benthic invertebrate, using the ubiquitous amphipod Orchomenella franklini (Walker, 1903. Seven microsatellite loci were used to assess genetic structure on three spatial scales: sites (100 s of metres, locations (1-10 kilometres and regions (1000 s of kilometres sampled in East Antarctica at Casey and Davis stations. Considerable genetic diversity was revealed, which varied between the two regions and also between polluted and unpolluted sites. Genetic differentiation among all populations was highly significant (F(ST = 0.086, R(ST = 0.139, p<0.001 consistent with the brooding mode of development in O. franklini. Hierarchical AMOVA revealed that the majority of the genetic subdivision occurred across the largest geographical scale, with N(em≈1 suggesting insufficient gene flow to prevent independent evolution of the two regions, i.e., Casey and Davis are effectively isolated. Isolation by distance was detected at smaller scales and indicates that gene flow in O. franklini occurs primarily through stepping-stone dispersal. Three of the microsatellite loci showed signs of selection, providing evidence that localised adaptation may occur within the Antarctic benthos. These results provide insights into processes of speciation in Antarctic brooders, and will help inform the design of spatial management initiatives recently endorsed for the Antarctic benthos.
Lucey, Noelle Marie; Lombardi, Chiara; Demarchi, Lucia; Schulze, Anja; Gambi, Maria Cristina; Calosi, Piero
Anthropogenic atmospheric carbon dioxide (CO2) is being absorbed by seawater resulting in increasingly acidic oceans, a process known as ocean acidification (OA). OA is thought to have largely deleterious effects on marine invertebrates, primarily impacting early life stages and consequently, their recruitment and species’ survival. Most research in this field has been limited to short-term, single-species and single-life stage studies, making it difficult to determine which taxa will be evolutionarily successful under OA conditions. We circumvent these limitations by relating the dominance and distribution of the known polychaete worm species living in a naturally acidic seawater vent system to their life history strategies. These data are coupled with breeding experiments, showing all dominant species in this natural system exhibit parental care. Our results provide evidence supporting the idea that long-term survival of marine species in acidic conditions is related to life history strategies where eggs are kept in protected maternal environments (brooders) or where larvae have no free swimming phases (direct developers). Our findings are the first to formally validate the hypothesis that species with life history strategies linked to parental care are more protected in an acidifying ocean compared to their relatives employing broadcast spawning and pelagic larval development.
Holstein, Daniel M.; Paris, Claire B.; Vaz, Ana C.; Smith, Tyler B.
Whether mesophotic reefs will behave as refugia for corals threatened by global climate change and coastal development depends on vertical exchange of larvae between diverse habitats. Here we use a biophysical model of larval dispersal to estimate vertical connectivity of a broadcasting ( Orbicella faveolata) and a brooding ( Porites astreoides) species of coral in the US Virgin Islands. Modeling predicts subsidy to shallow areas by mesophotic larvae of both species based on local hydrology, adult reproductive characteristics, larval traits, and a wide range of scenarios developed to test depth-sensitive factors, such as fertilization rates and post-settlement survivorship. In extreme model scenarios of reduced fertilization and post-settlement survivorship of mesophotic larvae, 1-10 % local mesophotic subsidy to shallow recruitment is predicted for both species, which are demographically significant. Although direct vertical connectivity is higher for the broadcaster, the brooder demonstrates higher local multigenerational vertical connectivity, which suggests that local P. astreoides populations are more resilient than those of O. faveolata, and corroborates field studies. As shallow habitat degrades, mesophotic-shallow subsidy is predicted to increase for both species. This study is the first of its kind to simulate larval dispersal and settlement between habitats of different depths, and these findings have local, regional, and global implications for predicting and managing coral reef persistence in a changing climate.
Full Text Available The response of Cobb and Ingham strain commercial broilers to a range of dietary amino acids in a factorial experiment (2 strains x 2 sexes x 3 dietary treatments with 3 replicates was evaluated. A total of 180 birds was sexed and weighed at one-day-old and randomly distributed to 36 brooders. Summit (246 g CP and 16.8 g lysine/kg and dilution (120 g CP and 6.0 g lysine/kg diets were prepared in isocaloric at 3100 kcal ME/kg. The estimated dietary level of crude protein for all birds at day-old was 240 g/kg and at 42 days were 120, 140 or 160 g/kg for females or 140, 160 or 180 g/kg for males. Body composition was measured at 42 days. Results revealed that strain Cobb males and females had a higher daily intake than Ingham, but Cobb females were less efficient in converting feed to weight gain than that of Ingham. The quantitative differences did not exist between genotypes, crude protein and lysine. Daily gain and feed utilization efficiency in both sexes were highest in group receiving the high protein diet. Lysine requirement was 14.58 g lysine /kg diet and 12.96 g/kg higher than NRC recommendation (1.05 % or 10.5 g/kg for maximum breast meat and lowest abdominal fat.
Full Text Available The study was conducted as a completely randomized design, with a factorial arrangement todetermine the response of commercial broilers to choice feeding and limiting amino acids on growth andcarcass performance. A total of 432 male birds were weighed at one-d-old and randomly distributed to48 wire-floored brooder cage each 1.0 m2. There were 2 sexes and 4 dietary treatments with 6 replicateseach of 9 birds. Birds were given one of three dietary regimens with dietary change every 7 days. Allgroups were fed free choice of summit and dilution diets. The estimated dietary level of crude protein atday-old was 240 g/kg and the level at 42 d was either 120, 150 or 180 g/kg for females or 130, 160 and190 g/kg for males. At 43 d of age, all birds from each dietary treatment were slaughtered formeasurement of body composition. Results reveal that lysine requirement for maximum gain in thisstudy was higher than NRC recommendation. The free choice-fed bird was significantly higher, in termsof growth and body composition than that obtained on the low dietary protein regimen.
The objective of this work was to determine the effects of feeding, housing and anti-parasitic treatment on the improvement of family poultry production in Morocco. Eight free range chicken flocks were partitioned into 2 groups; 'intervention' and 'no intervention' flocks. In 'Intervention' flocks, locally made brooders and poultry houses were provided, the birds received supplementary feeding and were subjected to anti-parasitic treatment against endo-parasites and ecto-parasites. Both categories of flocks were vaccinated against Newcastle disease and Gumboro disease. Data relative to flock size and structure, mortality, egg production, chicken and egg sales, sale prices, feed consumption were collected at a bi-weekly to monthly basis from July 2002 - July 2003 and were subjected to appropriate statistical comparisons of proportions and means. Flock size and structure and flock and egg production was significantly improved in 'intervention' flocks. Mortality of young birds (Chicks and cockerels/pullets) in 'intervention' flocks was significantly lower than in 'no intervention' flocks. The cost-benefit analysis has shown that 'intervention' flocks have made better profit. The mean relative profit (output/input ratio) in 'intervention' flocks was 3.65 and only 2.55 in 'no intervention' flocks. These results indicate that the performed interventions had an overall beneficial effect on the studied parameters. (author)
Venkatachalam Uthayakumar; Parathattil Rathan Sreedevi; Dhanapalan Senthilkumar; Subramanian Munirasu; Aathi Kiruba; Venkatachalam Ramasubramanian
Objective: To analyze the effect of seasonal variation and nutritional factors over the fecundity, gonado somatic index (GSI) and sperm motility of fresh water spiny eel Mastacembelus armatus (M. armatus) under artificial cave fitted tanks in laboratory condition. Methods: Forty pairs of M. armatus were collected from River Cauvery and are acclimated under lab condition. The fishes were fed with three different feeds like Fresh water prawns, earthworms and fish larvae for 60 days. Fecundity, sperm motility, Length weight relationship and GSI were analysed using standard procedures. Results: The fecundity rate was increased when body weight and gonad weight increased. The lograthemic relationship of the fecundity-total length and fecundity-body weight were found to be more correlated than the fecundity-gonad weight relationship. Maximum GSI was recorded in both male and female fishes during the breeding season (June-October). The condition factor (K) was maximum at the non breeding seasons and reduced in peak breeding season of M. armatus. In male fishes the sperm motility was recorded maximum (70%-85%) in the breeding season and regressed gradually to 0-15%. Among the feeding groups earthworm fed group showed higher body weight and fecundity rate than the control group which was statistically significant at P <0.05 level. Conclusion: The outcome of the present study shows the seasonal variation and feeding increase the reproductive performance. It can be effectively used for the healthy brooder selection, captive breeding and conservation of M. armatus.
Drake, P.; Arias, A. M.
The ichthyoplankton of a shallow inlet was sampled for 13 months using conical tide-strained nets. The utilization of this habitat by the young stages of different fish species is analysed. Temporal and spatial distributions are discussed in relation to environmental cycles and gradients, and to feeding rhythms. A total of 110 971 individuals, belonging to 39 species and 19 families of teleosts, was collected. The postlarval stage was the most represented in the samples. Ichthyoplankton density peaked during late winter and early spring. Density was higher during flood tide, resulting in a net input of postlarvae from the bay to the inner inlet. This ecosystem functions primarily as a nursery ground for coastal pelagic spawners and, secondarily, as a spawning area for some benthic egg spawners and pouch-brooder species. Most postlarvae migrate from their spawning area (open sea) to the inlet probably cued by a decreasing gradient of water temperature and salinity, and an increasing gradient of water turbidity and suitable food concentration. The attraction of postlarvae towards the inlet mouth probably results from a combination of biotic (availability of suitable food) and abiotic (temperature, salinity and turbidity) factors. Passive transport contributes to the subsequent penetration and retention of the postlarvae within the shallow, flood-dominated inlet. Vertical migrations observed among some planktonic fish stages were related to light periods and feeding activity rather than tidal phases, and cannot act as a retention mechanism.
Full Text Available The study was conducted as a completely randomized design, with a factorial arrangement to determine the response of commercial broilers to choice feeding and limiting amino acids on growth and carcass performance. A total of 432 male birds were weighed at one-d-old and randomly distributed to 48 wire-floored brooder cage each 1.0 m2. There were 2 sexes and 4 dietary treatments with 6 replicates each of 9 birds. Birds were given one of three dietary regimens with dietary change every 7 days. All groups were fed free choice of summit and dilution diets. The estimated dietary level of crude protein at day-old was 240 g/kg and the level at 42 d was either 120, 150 or 180 g/kg for females or 130, 160 and 190 g/kg for males. At 43 d of age, all birds from each dietary treatment were slaughtered for measurement of body composition. Results reveal that lysine requirement for maximum gain in this study was higher than NRC recommendation. The free choice-fed bird was significantly higher, in terms of growth and body composition than that obtained on the low dietary protein regimen.Keyword
Johnson, Nathan A.; Mcleod, John; Holcomb, Jordan; Rowe, Matthew T.; Williams, James D.
Accurate distribution data are critical to the development of conservation and management strategies for imperiled species, particularly for narrow endemics with life history traits that make them vulnerable to extinction. Medionidus walkeri is a rare freshwater mussel endemic to the Suwannee River Basin in southeastern North America. This species was rediscovered in 2012 after a 16-year hiatus between collections and is currently proposed for listing under the Endangered Species Act. Our study fills knowledge gaps regarding changes in distribution and early life history requirements of M. walkeri. Spatiotemporal changes in M. walkeri distribution were displayed using a conservation status assessment map incorporating metadata from 98 historical (1916–1999) and 401 recent (2000–2015) site surveys from museums and field notes representing records for 312 specimens. Recent surveys detected M. walkeri only in the middle Suwannee subbasin (n = 86, 22 locations) and lower Santa Fe subbasin (n = 2, 2 locations), and it appears the species may be extirpated from 67% of historically occupied 10-digit HUCs. In our laboratory experiments, M. walkeri successfully metamorphosed onPercina nigrofasciata (56.2% ± 8.9) and Etheostoma edwini (16.1% ± 7.9) but not on Trinectes maculatus, Lepomis marginatus, Notropis texanus, Noturus leptacanthus, Etheostoma fusiforme, orGambusia holbrooki. We characterize M. walkeri as a lure-displaying host fish specialist and a long-term brooder (bradytictic), gravid from fall to early summer of the following year. The early life history and distribution data presented here provide the baseline framework for listing decisions and future efforts to conserve and recover the species.
Pinzón, Jorge H C
Aim: Using high-resolution genetic markers on samples gathered from across their wide distributional range, we endeavoured to delimit species diversity in reef-building Pocillopora corals. They are common, ecologically important, and widespread throughout the Indo-Pacific, but their phenotypic plasticity in response to environmental conditions and their nearly featureless microskeletal structures confound taxonomic assignments and limit an understanding of their ecology and evolution. Location: Indo-Pacific, Red Sea, Arabian/Persian Gulf. Methods: Sequence analysis of nuclear ribosomal (internal transcribed spacer 2, ITS2) and mitochondrial (open reading frame) loci were combined with population genetic data (seven microsatellite loci) for Pocillopora samples collected throughout the Indo-Pacific, Red Sea and Arabian Gulf, in order to assess the evolutionary divergence, reproductive isolation, frequency of hybridization and geographical distributions of the genus. Results: Between five and eight genetically distinct lineages comparable to species were identified with minimal or no hybridization between them. Colony morphology was generally incongruent with genetics across the full range of sampling, and the total number of species is apparently consistent with lower estimates from competing morphologically based hypotheses (about seven or eight taxa). The most commonly occurring genetic lineages were widely distributed and exhibited high dispersal and gene flow, factors that have probably minimized allopatric speciation. Uniquely among scleractinian genera, this genus contains a monophyletic group of broadcast spawners that evolved recently from an ancestral brooder. Main conclusions: The delineation of species diversity guided by genetics fundamentally advances our understanding of Pocillopora geographical distributions, ecology and evolution. Because traditional diagnostic features of colony and branch morphology are proving to be of limited utility, the
Full Text Available Problem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, semen volume and chemical composition of seminal fluid during these sequential strippings. Approach: The 11 tagged males were stripped four times every 12-14 days with beginning of spermiation period (2 December 2008 towards its end (10 January 2008. One-way Analysis Of Variance (ANOVA was employed to analyze differences between means of semen parameters. Also, the relationships between semen parameters were tested using the bivariate correlation coefficients of Pearson. Results: The semen volume, sperm density, osmolality and the concentrations of Na+, Cl-, K+, Ca2+, Mg2+ and total protein gradually decreased whereas the values of glucose and triglyceride had no significant changes during sequential strippings. Also, the values of semen pH, the percentage (5s post-activation and duration of motility were statistically stable until third stripping but a decrease was recorded for these parameters in the fourth stripping. As well as, significant positive correlations were found for sperm density vs. K+, Cl-, Na+, Ca2+, Mg2+, total protein, spermatocrit; the percentage of motile spermatozoa Vs Ca2+, Mg2+, K+, Cl-, Na+, total protein and also the duration of motility Vs K+, Cl-, total protein and pH. Conclusion: The semen quality of Caspian brown trout males decrease in successive strippings during spawning season. Also, the knowledge on values and correlations between the sperm motility characteristics and the composition of seminal fluid could be useful to formulation of a species-specific extender solution for cryopreservation of semen of Caspian brown trout.
Betancourt, Liliana; Rodriguez, Fernando; Phandanouvong, Vienvilay; Ariza-Nieto, Claudia; Hume, Michael; Nisbet, David; Afanador-Téllez, German; Van Kley, Alexandra Martynova; Nalian, Armen
Essential oils have been proposed as alternatives to antibiotic use in food animal production. This study evaluated 3 chemotypes of the Origanum genus, containing varying amounts of secondary metabolites carvacrol, thymol, and sabinene, in the broiler chicken diet. Aerial parts of Origanum vulgare L. (OL), O. vulgare L. ssp. hirtum (OH), and O. majorana (OM) were collected from a greenhouse located in the high altitude Sabana de Bogotá (Savanna of Bogotá) and O. vulgare L. ssp. hirtum (OG) produced and ground in Greece. Oregano essential oils (OEO) from these plants were obtained by steam distillation and analyzed by gas chromatography coupled to a mass spectrometer. Six treatments were evaluated: 200 mg/kg of OEO from OH, OL, and OM, 50 mg/kg of OEO from OG, 500 mg/kg of chlortetracycline, and without additives. Broiler chicks were maintained at 2,600 m above sea level, placed in brooder cages under a completely randomized design. Template DNA was isolated from duodenal, jejunal, ileal, and cecal contents in each group and bacterial 16S rDNA patterns were analyzed by denaturing gradient gel electrophoresis. Dendrograms of denaturing gradient gel electrophoresis band patterns revealed 2 main clusters, OEO-treated chicks and nontreated control chicks, in each intestinal segment. Band patterns from different gut compartments revealed major bacterial population shifts in the foregut (duodenum, jejunum, and ileum) compared with the hindgut (cecum and colon) at all ages evaluated (P ascites was seen in additive-supplemented groups compared with the control group. This study represents the first work to evaluate the effects of the 3 main chemotypes of Origanum genus in broilers. PMID:25071230
Jones, J.W.; Neves, R.J.; Ahlstedt, S.A.; Hubbs, D.; Johnson, M.; Dan, H.; Ostby, B.J.K.
The life history and population demography of the endangered birdwing pearlymussel (Lemiox rimosus) were studied in the Clinch and Duck rivers, Tennessee. Reproducing populations of L. rimosus now occur only in the Clinch, Duck and Powell rivers, as the species is considered extirpated from the remaining portions of its range in the Tennessee River drainage. Females are long-term winter brooders, typically gravid from Oct. to May. Glochidia are contained in the outer gills and are released in association with a mantle-lure that resembles a small freshwater snail. Estimated fecundity, based on 8 gravid females collected from the Clinch and Duck rivers, ranged from 4132 to 58,700 glochidia/mussel. Seven fish species were tested for suitability as hosts for glochidia, and five darter species were confirmed through induced infestations: Etheostoma blennioides, E. camurum, E. rufilineatum, E. simoterum and E. zonale. Ages of L. rimosus shells were determined by thin-sectioning and ranged from 3 to 15 y in both rivers. Shell growth was higher and maximum size greater in males than females in both rivers. Shell growth was greatest in the Duck River. Densities of L. rimosus in the Clinch River were maintained at seemingly stable but low levels ranging from 0.07 to 0.27 m-2 from 20042007, and in the Duck River at similar but higher levels ranging from 0.6 to 1.0 m -2 from 20042006. In the latter river, abundance has increased since 1988, likely due to improved minimum flows and dissolved oxygen levels in water releases from a reservoir upstream. ?? 2010, American Midland Naturalist.
Jones, J.W.; Neves, R.J.; Ahlstedt, S.A.; Mair, R.A.
The reproduction, demography, and propagation of the endangered dromedary pearlymussel (Dromus dromas) (Lea, 1834) were studied in the Clinch and Powell rivers, Tennessee. Viable populations of the dromedary pearlymussel now occur only in the Clinch and Powell rivers; the species has been extirpated from the remaining portions of its range in the Cumberland and Tennessee river drainages. Females are long-term winter brooders, and they are gravid from October to June. Glochidia are contained in conglutinates that are red to white and resemble freshwater leeches or flatworms. Conglutinates are 20 to 40 mm long and are released through the excurrent aperture. Estimates of fecundity based on 7 gravid females collected from the Clinch River were 55,110 to 253,050 glochidia/mussel. The ages of 66 valves of D. dromas were determined by thin-sectioning and ranged from 3 to 25 y. Annual growth averaged 5 mm/y until age 10 and decreased to ???1.2 mm/ y thereafter. Nineteen fish species were tested for suitability as hosts for glochidia. Ten were confirmed as hosts through induced infestations of glochidia: black sculpin (Cottus baileyi), greenside darter (Etheostoma blennioides), fantail darter (Etheostoma flabellare), snubnose darter (Etheostoma simoterum), tangerine darter (Percina aurantiaca), blotchside logperch (Percina burtoni), logperch (Percina caprodes), channel darter (Percina copelandi), gilt darter (Percina evides), and Roanoke darter (Percina roanoka). Juveniles produced from these hosts were cultured in dishes held in nonrecirculating aquaculture systems containing fine sediment (<105 ??m) and were fed the green alga Nannochloropsis oculata every 2 d. Survival of 2810 newly metamorphosed juveniles was 836 (29.7%) after 1 to 2 wk.
Full Text Available Deep-water gorgonian corals are long-lived organisms found worldwide off continental margins and seamounts, usually occurring at depths of ∼200-1,000 m. Most corals undergo sexual reproduction by releasing a planktonic larval stage that disperses; however, recruitment rates and the environmental and biological factors influencing recruitment in deep-sea species are poorly known. Here, we present results from a 4-year field experiment conducted in the Gulf of Maine (northwest Atlantic at depths >650 m that document recruitment for 2 species of deep-water gorgonian corals, Primnoa resedaeformis and Paragorgia arborea. The abundance of P. resedaeformis recruits was high, and influenced by the structural complexity of the recipient habitat, but very few recruits of P. arborea were found. We suggest that divergent reproductive modes (P. resedaeformis as a broadcast spawner and P. arborea as a brooder may explain this pattern. Despite the high recruitment of P. resedaeformis, severe mortality early on in the benthic stage of this species may limit the abundance of adult colonies. Most recruits of this species (∼80% were at the primary polyp stage, and less than 1% of recruits were at stage of 4 polyps or more. We propose that biological disturbance, possibly by the presence of suspension-feeding brittle stars, and limited food supply in the deep sea may cause this mortality. Our findings reinforce the vulnerability of these corals to anthropogenic disturbances, such as trawling with mobile gear, and the importance of incorporating knowledge on processes during the early life history stages in conservation decisions.
Lacharité, Myriam; Metaxas, Anna
Deep-water gorgonian corals are long-lived organisms found worldwide off continental margins and seamounts, usually occurring at depths of ∼200-1,000 m. Most corals undergo sexual reproduction by releasing a planktonic larval stage that disperses; however, recruitment rates and the environmental and biological factors influencing recruitment in deep-sea species are poorly known. Here, we present results from a 4-year field experiment conducted in the Gulf of Maine (northwest Atlantic) at depths >650 m that document recruitment for 2 species of deep-water gorgonian corals, Primnoa resedaeformis and Paragorgia arborea. The abundance of P. resedaeformis recruits was high, and influenced by the structural complexity of the recipient habitat, but very few recruits of P. arborea were found. We suggest that divergent reproductive modes (P. resedaeformis as a broadcast spawner and P. arborea as a brooder) may explain this pattern. Despite the high recruitment of P. resedaeformis, severe mortality early on in the benthic stage of this species may limit the abundance of adult colonies. Most recruits of this species (∼80%) were at the primary polyp stage, and less than 1% of recruits were at stage of 4 polyps or more. We propose that biological disturbance, possibly by the presence of suspension-feeding brittle stars, and limited food supply in the deep sea may cause this mortality. Our findings reinforce the vulnerability of these corals to anthropogenic disturbances, such as trawling with mobile gear, and the importance of incorporating knowledge on processes during the early life history stages in conservation decisions. PMID:23762358
Exploiting the physiological and ecological characteristics of domesticated species has seldom been considered as a means of returning radiologically contaminated areas to safe agricultural production. However, the proper choice of cultivated and domesticated species, together with appropriate husbandry practices, may allow safe production of foods, fibre and energy. As an example, factors that could permit safe production of food products for human consumption from poultry raised in contaminated areas are considered. These factors include radionuclide transfer from the environment into poultry and methods for reducing radionuclide uptake and/or decontaminating chickens to yield acceptable food products. Studies of growth and feed intake rates of chickens under intensive management and free-ranging husbandries, 137Cs uptake by chickens exposed to contaminated sediments, potential effects of husbandry on 137Cs concentrations and 137Cs elimination by chickens after removal of contaminated feed are described. Data from these and other studies are combined in simulation models of the 13'7Cs kinetics of chickens. Chicken product 137Cs concentrations ([137Cs]s) decrease with increases in body mass, apparently as a result of decreasing mass specific intake rates. Husbandries that increase contaminant intake (e.g. free-ranging rearing conditions versus brooder house production) or access to contaminated soils (e.g. scattering feed directly onto contaminated solid versus the use of feeders) increase total body [137Cs]s. However, model simulations indicate that it is possible to produce safe poultry products (especially eggs) at all but unrealistically high feed contamination levels. Even when dietary 137Cs levels produce [137Cs]s in poultry products that exceed acceptable limits, the high metabolism of poultry leads to a rapid decrease in [137Cs]s once the chickens are placed on uncontaminated rations. This permits the use of contaminated feed or forage for most of the
DHANEESH Kottila Veettil; AJITH KUMAR Thipramalai Thankappan; SWAGAT Ghosh; BALASUBRAMANIAN Thangavel
Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater.We designed an indoor program of A.percula culture in brackishwater with a salinity of 24±1,during which the impacts of feed type,water temperature,and light intensity,on the efficiency of its reproduction,were revealed.The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency.Higher reproductive efficiency [number of eggs laid (276±22.3 eggs)] was observed when fish were fed live Acetes sp.rather than clam (204±16.4eggs),trash fish (155±12 eggs) and formulated feed (110±10 eggs).The spawning rate was increased during September and October (water temperature,28.74±0.55℃) on average of 2.4 spawning per month; and low spawning rate was in January (water temperature,24.55±0.45℃) on average of 1 spawning per month.Among three light intensities (100,500,and 900 Ix) set to evaluate larval survival rate,larvae showed the highest survival rate (65.5％) at 900 Ix.The breeding method specifically in brackishwater developed in the present study is a new approach,will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.
Rao, S V Rama; Prakash, B; Raju, M V L N; Panda, A K; Kumari, R K; Reddy, E Pradeep Kumar
Two experiments were conducted to study the effect of supplementing organic forms of zinc (Zn), selenium (Se) and chromium (Cr) on performance, anti-oxidant activities and immune responses in broiler chickens from 1 to 21 days of age, which were reared in cyclic heat-stressed condition under tropical summer in open-sided poultry house. A total of 200 (experiment I) and 450-day-old (experiment II) broiler male chicks (Cobb 400) were randomly distributed in stainless steel battery brooders (610 mm × 762 mm × 475 mm) at the rate of five birds per pen. A maize-soybean meal-based control diet (CD) containing recommended (Vencobb 400, Broiler Management Guide) concentrations of inorganic trace minerals and other nutrients was prepared. The CD was supplemented individually with organic form of selenium (Se, 0.30 mg/kg), chromium (Cr, 2 mg/kg) and zinc (Zn, 40 mg/kg) in experiment I. In experiment II, two concentrations of each Zn (20 and 40 mg/kg), Se (0.15 and 0.30 mg/kg) and Cr (1 and 2 mg/kg) were supplemented to the basal diet in 2 × 2 × 2 factorial design. A group without supplementing inorganic trace minerals was maintained as control group in both experiments. Each diet was allotted randomly to ten replicates in both experiments and fed ad libitum from 1 to 21 days of age. At 19th day of age, blood samples were collected for estimation of anti-oxidant and immune responses. Supplementation of Se, Cr and Zn increased (P 0.05) by the interaction between levels of Zn, Se and Cr in broiler diet. The FE improved (P 0.05) the immune responses (Newcastle disease titre and cell-mediated immune response to phytohaemagglutinin-P) in both the experiments. Based on the results, it is concluded that supplementation of organic form of Se, Cr and Zn (0.30, 2 and 40 mg/kg, respectively) either alone or in combination significantly improved performance and anti-oxidant responses (reduced LP and increased superoxide dismutase) in commercial
Aspectos reproductivos del acocil Cambarellus (Cambarellus montezumae (Crustacea: Decápoda: Cambaridae en condiciones controladas Breeding aspects of the crayfish Cambarellus (Cambarellus montezumae (Crustacea: Decapoda: Cambaridae under controlled conditions
José Luis Arredondo-Figueroa
Full Text Available El acocil Cambarellus (C. montezumae es una especie endémica cuya distribución está restringida a algunos cuerpos de agua lacustre. Se presentan los resultados sobre aspectos reproductivos de esta especie en condiciones controladas, con el objetivo de determinar la factibilidad de la producción de huevos y juveniles y su potencial aplicación a programas de rescate, repoblación y cultivo. Se realizaron 2 ensayos experimentales, el primero en 4 estanques exteriores de 800 litros conectados a sistemas cerrados de recirculación (SCR y el segundo en recipientes de plástico en laboratorio (RPL, de 2 litros. Para el primer ensayo, se manejaron 219 reproductores durante 335 días, obteniendo 136 hembras ovígeras. Cada hembra produjo en promedio 45 ± 23 huevos por puesta. El 83.7% de los huevos eclosionaron, obteniéndose un total de 3 162 juveniles. En los RPL se manejó una población F1 obtenida a partir de los reproductores del SCR en una relación 1 hembra: 2 machos, con 10 repeticiones. La producción de huevo fue variable con un promedio de 34 por hembra. Los indicadores de desempeño mostraron diferencias significativas (P ≤ 0.05 entre sexos con respecto a 6 de ellos. Los resultados indican la factibilidad para su cultivo y producción de huevos y juveniles.Cambarellus (C. montezumae is an endemic crayfish from Mexico, which actual distribution is restricted to few lacustrine bodies of water. Here we present results on the reproduction of this species under controlled conditions with the aim to determine the feasibility of the production of eggs and juveniles, as an strategy for their rescue, re-population and culture. Two experimental assays were conducted, the first one, in 4 outdoor 800-L tanks recirculation aquaculture systems (RAS and the second, in 2-L plastic containers under laboratory conditions (PCL. For the first assay, 219 brooders were used during 335 days , obtaining 136 ovigerous females. Each female produced an average
Cabral-Tena, R. A.; Sánchez, A.; Reyes-Bonilla, H.; Ruvalcaba-Díaz, A. H.; Balart, E. F.
Coral δ18O variations are used as a proxy for changes in near sea surface temperature and seawater isotope composition. Skeletal δ13C of coral is frequently used as a proxy for solar radiation because most of its variability is controlled by an interrelationship between three processes: photosynthesis, respiration, and feeding. Coral growth rate is known to influence the δ18O and δ13C isotope record to a lesser extent. Recent published data show differences in growth parameters between female and male coral; thus, skeletal δ18O and δ13C are hypothesized to be different in each sex. To assess this difference, this study describes changes in the skeletal δ18O and δ13C record of four female and six male Porites panamensis coral collected in Bahía de La Paz, whose growth bands spanned 12 years. The isotopic data were compared to SST, precipitation, PAR, chlorophyll a, and skeletal growth parameters. Porites panamensis is a known gonochoric brooder whose growth parameters are different in females and males. Splitting the data by sexes explained 81 and 93 % of the differences of δ18O, and of δ13C, respectively, in the isotope record between colonies. Both isotope records were different between sexes. δ18O was higher in female colonies than in male colonies, with a 0.31 ‰ difference; δ13C was lower in female colonies, with a 0.28 ‰ difference. A difference in the skeletal δ18O implies an error in SST estimates of ≈ 1.0 °C to ≈ 2.6 °C. The δ18O records showed a seasonal pattern that corresponded to SST, with low correlation coefficients (-0.45, -0.32), and gentle slopes (0.09 ‰ °C-1, 0.10 ‰ °C-1) of the δ18O-SST relation. Seasonal variation in coral δ18O represents only 52.37 and 35.66 % of the SST cycle; 29.72 and 38.53 % can be attributed to δ18O variability in seawater. δ13C data did not correlate with any of the environmental variables; therefore, variations in skeletal δ13C appear to be driven mainly by metabolic effects. Our
Powell, S; Bidner, T D; Southern, L L
An experiment was conducted to investigate the effect of dietary Ca level on the efficacy of phytase. A total of 288 male Ross × Ross 708 broilers with initial and final BW of 37 and 705 g, respectively, were used in brooder batteries from 0 to 21 d posthatch. Each treatment had 8 replications with 6 broilers/replicate pen. All diets were corn-soybean meal based and formulated to contain 1.26% total Lys. The treatments were positive control with 0.45% nonphytate P and 1% Ca and a negative control with 0.20% nonphytate P with 0.67, 1.00, or 1.33% Ca fed with or without 500 phytase units of Optiphos (Escherichia coli-derived phytase; JBS United Inc., Sheridan, IN). Increasing Ca from 0.67 to 1.33% linearly decreased (P ≤ 0.003) ADG, ADFI, bone breaking strength, bone weight, tibia ash weight, and percentage tibia ash; however, quadratic effects were found for ADFI, G:F, percentage tibia ash, and mortality (P ≤ 0.09). Phytase supplementation increased (P < 0.001) ADG, ADFI, bone breaking strength, bone weight, ash weight, and percentage tibia ash and decreased (P = 0.054) mortality. The increase in ADG, ADFI, bone weight, ash weight, and percentage tibia ash (P ≤ 0.026) and decrease in mortality (phytase × Ca linear; P = 0.058) from phytase supplementation was greater in broilers fed the higher levels of Ca. Calcium utilization was linearly decreased (P < 0.002) with increasing Ca. Phosphorus digestibility and utilization were increased with increasing levels of Ca (P ≤ 0.002); however, P utilization decreased at 1% Ca and increased at 1.33% (quadratic; P < 0.070). Phytase supplementation increased Ca utilization (P < 0.024), P digestibility (P < 0.001), and P utilization (P < 0.029). However, the increase in P digestibility (phytase × Ca; P < 0.021) was greater at the lower levels of Ca whereas P utilization (phytase × Ca; P < 0.001) was greater at 1.33% Ca with phytase supplementation. The results of this research indicate that dietary Ca level, within
Full Text Available Broiler introduction and adaptation trial was done at Bahir Dar city in the year 2011/2012. The major objective of the trial was to introduce broiler breeds in to the region and evaluate their performance at smallholder level. A total of 1100 day old chicks of Hubbard Classical breed were purchased from Debre Zeit Agricultural Research Center and transported to Bahir Dar. Each participant received, on average, 109 day old chicks and 400kg started and finisher feed. Brooding was done using electrical brooder. Finished broilers were sold live and in processed form after six weeks of age. The average weight old day old chicks was 45.5g. The average weight of birds at end of 1st, 2nd, 3rd, 4th, 5th and 6th weeks were 136g, 364g, 711g, 1174g, 1665g and 2092g, respectively. The average final weight was 2092g (ranged 1957g - 2216g. The average cumulative mortality was 4.8% (Ranged 1.9%-6.5%. The average daily feed intake and cumulative feed intake of each bird during the entire period was 99g and 4052g, respectively. The average FCR was 1.84. The partial budget analysis result indicated that broiler production was profitable with a net benefit of 10.75 Eth Birr/head and 19.3 Ethi0pian Birr/kg, respectively. The result showed that participants who sold processed broiler meat fetched 8.6 Eth Birr/kg more than those participants who sold live birds. The survey result indicated that all participants were highly satisfied by the breed. According to the producers; the breed has paramount merit than other chicken breeds like; very fast growth, high meat production and short rearing period. The higher final body weight, the lower mortality, higher profitability and higher market demand revealed that broilers could be reared at small holder level in big cities like Bahir Dar. The result of the trial showed that actions should be taken to solve the prevailing constraint including; lack of day old chicks and quality feed. One of the main lessons we learned from
Máca, Ondřej; Pavlásek, Ivan
From July to November 2012, preliminary coprological examinations were carried out on 85 pooled faecal samples of different aged ring-necked pheasants (Phasianus colchicus) (hatches from May until July) from an intensive artificial breeding programme in the Czech Republic. Cryptosporidium oocysts were detected in 12 samples (14.1 %) of ages >12 weeks (August-September). These results were supported by findings of Cryptosporidium baileyi and Cryptosporidium meleagridis oocysts in intestinal, or cloacal contents, and/or the bursa of Fabricius in 9 from 36 examined dead pheasants (prevalence 25 %). We describe in detail the various age groups of pheasants after hatching and present graphically the overall results of coprological examinations, showing pathways of infection of C. baileyi and C. meleagridis during the full rearing seasons of 2013 and 2014. We found very similar mean proportions of Cryptosporidium-positive samples over the entire 2013 period in pheasantry (173 pooled samples tested, 25 positive, 14.5 %) and 2014 (238 samples tested, 43 positive, 18.1 %). All tests were verified as being Cryptosporidium positive in 9 from 219 (prevalence 4.1 %) and 4 from 168 (prevalence 2.4 %) post-mortem examinations. Significantly, C. baileyi was found more frequently in faeces, with positivities ranging from 11.1 to 100 % (4->16-week-old pheasants). Oocysts of C. meleagridis were detected at ages 6->15 weeks ranging from 7.1 to 100 % in faeces during the rearing seasons. The burdens of C. baileyi (7 of 14 and 10 of 16) and C. meleagridis (5 of 14 and 7 of 16) for each year, in monitored brooder houses, flight pens and spread across all open areas were recorded. Oocysts of C. baileyi and C. meleagridis obtained from this study, and Cryptosporidium galli (obtained in another aviary from 36-week-old pheasants), were sequenced, and we characterized the highly variable 60-kDa glycoprotein gene of C. meleagridis. These results highlight the real risk of
Paulo Giovanni de Abreu
plate with plastic sheet cover, and brooder heating gas. Heating was maintained up to the 21st days of age, beginning with the temperature of 35ºC in the first week with a reducing of 3ºC by each week. Live weight, weight gain, feed intake, feed:gain ratio and mortality were evaluated. The electric energy and gas expenditures were recorded during all bird heating period in order to compare the different heating systems cost. The heat electric system with mortar structure plate and plastic sheet cover was the most indicated, by promoting good thermal conditions and higher gross margin at the end of the growing period.
Acondicionamiento de reproductores, desove y cultivo larval de Graus nigra (Philippi, 1887 (Kyphosidae: Girellinae Broodstock conditioning, spawning and larval culture of Graus nigra (Philippi, 1887 (Kyphosidae: Girellinae
Full Text Available Se describen resultados sobre acondicionamiento reproductivo, desove y cultivo larval de Graus nigra ("vieja negra", "mulata". Peces adultos silvestres se recolectaron y se utilizaron como reproductores, los que al final del período de acondicionamiento alcanzaron el estado de maduración gonadal y desovaron en forma natural y espontánea. Los huevos fueron recolectados y después de 36 h de incubación eclosionaron, con una tasa de eclosión promedio de 60%. Las larvas recién eclosionadas midieron 2,9 ± 0,23 mm y alcanzaron el día 50 post-eclosión (PE una longitud total de 12,6 ± 0,37 mm. La sobrevivencia larval posterior a la eclosión fue entre 50,9 y 79,1% y al día 30 PE fue de 12,1%. El cultivo larval se desarrolló en estanques con suministro de agua de mar filtrada y esterilizada. Después de la reabsorción del saco vitelino se produjo el desarrollo del tracto digestivo y las larvas se alimentaron con dieta viva enriquecida con emulsión de ácidos grasos altamente insaturados. A los 35 días de cultivo se ofreció alimento artificial a las larvas cuyo tamano fue aumentando progresivamente a medida que progresó su desarrollo ontogénico. Se describe la evolución anatómica de las larvas y las relaciones morfométricas que representan su desarrollo; se caracteriza el patrón de crecimiento de las larvas hasta los 50 días post-eclosión y se discuten aspectos relacionados con la sobrevivencia larval y la introducción de mejoras para optimizar la producción de larvas y juveniles.In this study results related to reproductive conditioning, spawning and larval culture of Graus nigra ("vieja negra", "mulata" are given. Wild adult fishes were collected and used as brooders which at the end of the conditioning period reached gonadal maturation state and spawned naturally and spontaneously. Eggs were collected and after 36 hours of incubation they hatched at average rate of 60%. The hatched larvae measured 2.9 ± 0.23 mm and at day 50
Full Text Available El pez de roca Graus nigra, comúnmente llamado "mulata" o "vieja negra" es un recurso importante a lo largo de la costa centro y norte de Chile. Debido a la gran aceptación como pescado para consumo humano, han sido intensamente extraídos por lo cual es necesario el desarrollo de su producción por medio de técnicas de acuicultura. En este estudio se reportan los resultados de crecimiento y alimentación de juveniles producidos en laboratorio. Los peces juveniles se obtuvieron a partir de reproductores silvestres, a quienes se les aplicó técnicas estándar de criadero. El cultivo de peces juveniles se desarrolló en estanques con suministro de agua de mar sin filtrar y con aireación constante. El proceso de deshabituación se extendió por 23 días y entre los 70 y 205 días post-eclosión (PE, los juveniles experimentan un crecimiento alométrico, con una sobrevivencia de 10,2%, con una tasa específica de crecimiento (SGR en longitud total de 1,02%/día y en peso total de 3,07%/día, con una conversión de alimento aparente (CAa y biológica (CAb de 3,02 y 2,69 respectivamente para el último periodo en estudio (190 a 205 PE. Este primer intento de producir juveniles fue prometedor y abre las posibilidades de un cultivo futuro.The rocky fish (Graus nigra commonly named "mulata" is an important resource along the coast of central and northern Chile. Due to great acceptation as fish for human consumption have been intensively fished so as it's needed develop its production by means of aquaculture techniques. First attempts for culturing this species have given promising results with regard to production of larvae and juvenile fish. In this study results of growth and feeding of hatchery produced juveniles are reported. Fish juveniles were generated using wild brooders by means of application standard techniques of hatchery. Cultivation of juvenile fish was developed in tanks supplied with not filtered seawater and provided with constant
Asociación del color de la concha de reproductores de Argopecten purpuratus (Lamarck, 1819 con la supervivencia, crecimiento y desarrollo larval de sus progenies Association between shell color of breeds (Lamarck, 1819 and the survival, growth and larval development of their progenies
Ricardo M García
most frequent shell colors. Knowing that Argopecten purpuratus shell color variation is under genetic control, in this work we test the hypothesis that loci that control shell color variation also affect larvae's growth rate, survival and/or development rate. Survival, growth and development rates were estimated in larvae produced in different crosses between brooders of A. purpuratus having low frequency shell colors (white or orange and "normal" (brown color. Results showed no significant differences in growth rates between larvae produced by crosses that involved brown shell parents, or orange by white parents. However, progenies of self fertilized orange and white parents showed significant differences in growth among them, and a lower growth rate than the remaining crosses. Results suggest that genes that control shell color variation in juvenile and adult A. purpuratus could affect the growth rates of their larvae, but not the development rate or survival.
Cabral-Tena, Rafael A.; Sánchez, Alberto; Reyes-Bonilla, Héctor; Ruvalcaba-Díaz, Angel H.; Balart, Eduardo F.
Coral δ18O variations are used as a proxy for changes in sea surface temperature (SST) and seawater isotope composition. Skeletal δ13C of coral is frequently used as a proxy for solar radiation because most of its variability is controlled by an interrelationship between three processes: photosynthesis, respiration, and feeding. Coral growth rate is known to influence the δ18O and δ13C isotope record to a lesser extent than environmental variables. Recent published data show differences in growth parameters between female and male coral in the gonochoric brooding coral Porites panamensis; thus, skeletal δ18O and δ13C are hypothesized to be different in each sex. To test this, this study describes changes in the skeletal δ18O and δ13C record of four female and six male Porites panamensis coral collected in Bahía de La Paz, Mexico, whose growth bands spanned 12 years. The isotopic data were compared to SST, precipitation, photosynthetically active radiation (PAR), chlorophyll a, and skeletal growth parameters. Porites panamensis is a known gonochoric brooder whose growth parameters are different in females and males. Splitting the data by sexes explained 81 and 93 % of the differences of δ18O, and of δ13C, respectively, in the isotope record between colonies. Both isotope records were different between sexes. δ18O was higher in female colonies than in male colonies, with a 0.31 ‰ difference; δ13C was lower in female colonies, with a 0.28 ‰ difference. A difference in the skeletal δ18O could introduce an error in SST estimates of ≈ 1.0 to ≈ 2.6 °C. The δ18O records showed a seasonal pattern that corresponded to SST, with low correlation coefficients (-0.45, -0.32), and gentle slopes (0.09, 0.10 ‰ °C-1) of the δ18O-SST relation. Seasonal variation in coral δ18O represents only 52.37 and 35.66 % of the SST cycle; 29.72 and 38.53 % can be attributed to δ18O variability in seawater. δ13C data did not correlate with any of the environmental
Kleiber, M; Dougherty, J E
1. An optimum of environmental temperature is to be expected for the utilization of food energy in warm blooded animals if their food intake is determined by their appetite. 2. Baby chicks were kept in groups of five chicks in a climatic cabinet at environmental temperatures of 21 degrees , 27 degrees , 32 degrees , 38 degrees , and 40 degrees C. during the period of 6 to 15 days of age. The intake of qualitatively complete food was determined by their appetite. Food intake, excretion, and respiratory exchange were measured. Control chicks from the same hatch as the experimental groups were raised in a brooder and were given the same food as the experimental chicks. The basal metabolism of each experimental group was determined from 24 to 36 hours without food at the age of 16 days. 3. The daily rate of growth increased with decreasing environmental temperature from 2.74 gm. at 40 degrees C. to 4.88 gm. at 21 degrees C. This was 4.2 to 6.5 per cent of their body weight. 4. The amount of food consumed increased in proportion to the decrease in temperature. 5. The availability of the food, used for birds instead of the digestibility and defined as See PDF for Structure showed an optimum at 38 degrees C. 6. The CO(2) production increased from 2.95 liters CO(2) per day per chick at 40 degrees C. to 6.25 liters at 21 degrees C. Per unit of the 3/4 power of the body weight, 23.0 liters CO(2) per kilo(3/4) was produced at 40 degrees C. and 43.4 liters per kilo(3/4) at 21 degrees C. The CO(2) production per unit of 3/4 power of the weight increased at an average rate of approximately 1 per cent per day increase in age. The R.Q. was, on the average, 1.04 during the day and 0.92 during the night. 7. The net energy is calculated on the basis of C and N balances. A maximum of 11.8 Cal. net energy per chick per day was found at 32 degrees C. At 21 degrees C. only 6.9 Cal. net per day per chick was produced and at 40 degrees C. an average of 6.7 Cal. 8. The composition of the