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Sample records for bronchiolar clara ciliated

  1. Clara cell adhesion and migration to extracellular matrix

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    deMello Daphne

    2008-01-01

    Full Text Available Abstract Background Clara cells are the epithelial progenitor cell of the small airways, a location known to be important in many lung disorders. Although migration of alveolar type II and bronchiolar ciliated epithelial cells has been examined, the migratory response of Clara cells has received little attention. Methods Using a modification of existing procedures for Clara cell isolation, we examined mouse Clara cells and a mouse Clara-like cell line (C22 for adhesion to and migration toward matrix substrate gradients, to establish the nature and integrin dependence of migration in Clara cells. Results We observed that Clara cells adhere preferentially to fibronectin (Fn and type I collagen (Col I similar to previous reports. Migration of Clara cells can be directed by a fixed gradient of matrix substrates (haptotaxis. Migration of the C22 cell line was similar to the Clara cells so integrin dependence of migration was evaluated with this cell line. As determined by competition with an RGD containing-peptide, migration of C22 cells toward Fn and laminin (Lm 511 (formerly laminin 10 was significantly RGD integrin dependent, but migration toward Col I was RGD integrin independent, suggesting that Clara cells utilize different receptors for these different matrices. Conclusion Thus, Clara cells resemble alveolar type II and bronchiolar ciliated epithelial cells by showing integrin mediated pro-migratory changes to extracellular matrix components that are present in tissues after injury.

  2. Novel method for isolation of murine clara cell secretory protein-expressing cells with traces of stemness.

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    Xiao-Yang Wang

    Full Text Available Clara cells are non-ciliated, secretory bronchiolar epithelial cells that serve to detoxify harmful inhaled substances. Clara cells also function as stem/progenitor cells for repair in the bronchioles. Clara cell secretory protein (CCSP is specifically expressed in pulmonary Clara cells and is widely used as a Clara cell marker. In addition CCSP promoter is commonly used to direct gene expression into the lung in transgenic models. The discovery of CCSP immunoreactivity in plasma membranes of airway lining cells prompted us to explore the possibility of enriching Clara cells by flow cytometry. We established a novel and simple method for the isolation of CCSP-expressing cell Clara cells using a combination of mechanical and enzymatic dissociation followed by flow cytometry sorting technology. We showed that ∼25% of dissociated cells from whole lung expressed CCSP. In the resulting preparation, up to 98% of cells expressed CCSP. Notably, we found that several common stem cell markers including CD44, CD133, Sca-1 and Sox2 were expressed in CCSP(+ cells. Moreover, CCSP(+ cells were able to form spheroid colonies in vitro with 0.97‰ efficiency. Parallel studies in vivo confirmed that a small population of CCSP(-expressing cells in mouse airways also demonstrates stem cell-like properties such as label retention and harboring rare bronchioalveolar stem cells (BASCs in terminal bronchioles (TBs. We conclude that CCSP(+ cells exhibit a number of stem cell-like features including stem cell marker expression, bronchosphere colony formation and self-renewal ability. Clara cell isolation by flow cytometry sorting is a useful method for investigating the function of primary Clara cells in stem cell research and mouse models.

  3. E-cadherin Controls Bronchiolar Progenitor Cells and Onset of Preneoplastic Lesions in Mice

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    Fatih Ceteci

    2012-12-01

    Full Text Available Although progenitor cells of the conducting airway have been spatially localized and some insights have been gained regarding their molecular phenotype, relatively little is known about the mechanisms regulating their maintenance, activation, and differentiation. This study investigates the potential roles of E-cadherin in mouse Clara cells, as these cells were shown to represent the progenitor/stem cells of the conducting airways and have been implicated as the cell of origin of human non-small cell lung cancer. Postnatal inactivation of E-cadherin affected Clara cell differentiation and compromised airway regeneration under injury conditions. In steady-state adult lung, overexpression of the dominant negative E-cadherin led to an expansion of the bronchiolar stem cells and decreased differentiation concomitant with canonical Wnt signaling activation. Expansion of the bronchiolar stem cell pool was associated with an incessant proliferation of neuroepithelial body.associated Clara cells that ultimately gave rise to bronchiolar hyperplasia. Despite progressive hyperplasia, only a minority of the mice developed pulmonary solid tumors, suggesting that the loss of E-cadherin function leads to tumor formation when additional mutations are sustained. The present study reveals that E-cadherin plays a critical role in the regulation of proliferation and homeostasis of the epithelial cells lining the conducting airways.

  4. Bronchiolar disease: spectrum and radiological findings

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    Takahashi, Masashi; Murata, Kiyoshi; Takazakura, Ryutaro; Nakahara, Tetsuro; Shimizu, Kentaro; Minese, Mariko; Itoh, Harumi

    2000-07-01

    Two types of bronchiole, the terminal bronchiole and the respiratory bronchiole, have structural and functional differences. The former is characterized as a conducting airway and the latter is closely related to a gas-exchange function as it has numerous alveoli on the wall. Therefore, the diseases occurring at bronchiole demonstrate different pathological, radiological and clinical pictures depending on which bronchiole is mainly involved. The disease that mainly involves the conducting airway is appreciated as a small airway disease. Constrictive bronchiolitis is a well-recognized entity classified in this category. Whereas the disease mainly involves the respiratory bronchiole and distal alveolar space, it is recognized as an interstitial and parenchymal disease. BOOP or RB-ILD is classified in this category. These two types of bronchiolar diseases reveal the contrast clinical pictures, including incidence, causative disease, response to the treatment, prognosis, respiratory function test as well as the radiological findings. This pictorial essay will illustrate the radiological features of the varieties of bronchiolitis.

  5. Clara's birth.

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    Thorens, S; Richer, D; Bel, A; Bel, B

    1999-01-01

    Advocacy for homebirth is based on the strong assumption that birthing is a physiological process and does not require medical interventions unless things turn "wrong." Let us assume that something might always go wrong, for instance during Clara's birth when the placenta was still retained after three hours. What needs to be done? The moment the midwife entered the house she was endowed with a responsibility for any problem caused by her failure to give proper guidance. With this weight on her shoulder, and according to her training and experience, there was no other way for her than to suggest an intervention regarding the placenta. The two midwives, B, and C., might not agree on risk estimations, the nature of the intervention, whether it should be performed at home or in a hospital. The estimation of abnormalities, evaluation of risks and the procedures with which to handle them are the main practical difference between classic obstetrics and non-interventionist midwifery--by analogy, between allopathy and naturopathy. The rest (positive thinking) is basically literature. A delivery will not remain normal just because we decide it "must" be physiological. Dr. Barua, a professor of obstetrics in Pondicherry, pointed out that normal deliveries are rare--fewer than 10 percent in South India. What we have instead is either pathological or "natural" deliveries in which regenerative processes take care of abnormal situations. Unless she has developed sensitive hands, a birth assistant or midwife must rely on monitoring procedures to evaluate deviations from the normal process. Even with the greatest care, these procedures are intrusive in that they disconnect the parturient from her own sensations. While successful unattended homebirth stories emphasise the extraordinary power and sensitivity of a birthing woman, the whole dream seems to collapse in abnormal or pathological cases. It would have collapsed for Sonia as well, had she not discarded negative suggestions

  6. Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation

    Science.gov (United States)

    2010-01-01

    Background Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. The purpose of the study was to investigate the role of elemental carbon ultrafine particles (EC-UFP)-induced oxidative stress on Clara cells and CC16 in a mouse model of allergic lung inflammation. Methods Ovalbumin (OVA)-sensitized mice were exposed to EC-UFP (507 μg/m3 for 24 h) or filtered air immediately prior to allergen challenge and systemically treated with N-acetylcysteine (NAC) or vehicle prior and during EC-UFP inhalation. CC16 was measured up to one week after allergen challenge in bronchoalveolar lavage fluid (BALF) and in serum. The relative expression of CC16 and TNF-α mRNA were measured in lung homogenates. A morphometrical analysis of mucus hypersecretion and electron microscopy served to investigate goblet cell metaplasia and Clara cell morphological alterations. Results In non sensitized mice EC-UFP inhalation caused alterations in CC16 concentration, both at protein and mRNA level, and induced Clara cell hyperplasia. In sensitized mice, inhalation of EC-UFP prior to OVA challenge caused most significant alterations of BALF and serum CC16 concentration, BALF total protein and TNF-α relative expression compared to relevant controls; their Clara cells displayed the strongest morphological alterations and strongest goblet cell metaplasia occurred in the small airways. NAC strongly reduced both functional and morphological alterations of Clara cells. Conclusion Our findings demonstrate that oxidative stress plays an important role in EC-UFP-induced augmentation of functional and morphological alterations of Clara cells in allergic lung inflammation. PMID:20420656

  7. Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation.

    Science.gov (United States)

    Alessandrini, Francesca; Weichenmeier, Ingrid; van Miert, Erik; Takenaka, Shinji; Karg, Erwin; Blume, Cornelia; Mempel, Martin; Schulz, Holger; Bernard, Alfred; Behrendt, Heidrun

    2010-04-26

    Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. The purpose of the study was to investigate the role of elemental carbon ultrafine particles (EC-UFP)-induced oxidative stress on Clara cells and CC16 in a mouse model of allergic lung inflammation. Ovalbumin (OVA)-sensitized mice were exposed to EC-UFP (507 microg/m(3) for 24 h) or filtered air immediately prior to allergen challenge and systemically treated with N-acetylcysteine (NAC) or vehicle prior and during EC-UFP inhalation. CC16 was measured up to one week after allergen challenge in bronchoalveolar lavage fluid (BALF) and in serum. The relative expression of CC16 and TNF-alpha mRNA were measured in lung homogenates. A morphometrical analysis of mucus hypersecretion and electron microscopy served to investigate goblet cell metaplasia and Clara cell morphological alterations. In non sensitized mice EC-UFP inhalation caused alterations in CC16 concentration, both at protein and mRNA level, and induced Clara cell hyperplasia. In sensitized mice, inhalation of EC-UFP prior to OVA challenge caused most significant alterations of BALF and serum CC16 concentration, BALF total protein and TNF-alpha relative expression compared to relevant controls; their Clara cells displayed the strongest morphological alterations and strongest goblet cell metaplasia occurred in the small airways. NAC strongly reduced both functional and morphological alterations of Clara cells. Our findings demonstrate that oxidative stress plays an important role in EC-UFP-induced augmentation of functional and morphological alterations of Clara cells in allergic lung inflammation.

  8. Analysis of Cell Turnover in the Bronchiolar Epithelium Through the Normal Aging Process.

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    Ortega-Martínez, Marta; Rodríguez-Flores, Laura E; Ancer-Arellano, Adriana; Cerda-Flores, Ricardo M; de-la-Garza-González, Carlos; Ancer-Rodríguez, Jesús; Jaramillo-Rangel, Gilberto

    2016-08-01

    Aging is associated with changes in the lung that leads to a decrease in its function. Alterations in structure and function in the small airways are well recognized in chronic lung diseases. The aim of this study was the assessment of cell turnover in the bronchiolar epithelium of mouse through the normal aging process. Lungs from CD1 mice at the age of 2, 6, 12, 18, or 24 months were fixed in neutral-buffered formalin and paraffin-embedded. Proliferating cell nuclear antigen was examined by immunohistochemistry. Apoptosis was analyzed by in situ end-labeling of fragmented DNA. Epithelial dimensions were analyzed by morphometry. The 2-month-old mice showed significantly higher number of proliferating cells when compared with mice at all other age groups. The number of apoptotic cells in mice at 24 months of age was significantly greater than in mice at all other age groups. Thus, the number of epithelial cells decreased as the age of the subject increased. We also found reductions in both area and height of the bronchiolar epithelium in mice at 18 and 24 months of age. We found a decrease in the total number of epithelial cells in the aged mice, which was accompanied by a thinning of the epithelium. These changes reflect a dysregulated tissue regeneration process in the bronchiolar epithelium that might predispose to respiratory diseases in elderly subjects.

  9. Epigenetic inheritance in ciliates.

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    Nowacki, Mariusz; Landweber, Laura F

    2009-12-01

    2009 marks not only the 200th anniversary of Darwin's birth but also publication of the first scientific evolutionary theory, Lamarck's Philosophie Zoologique. While Lamarck embraced the notion of the inheritance of acquired characters, he did not invent it (Burkhardt, 1984). New phenomena discovered recently offer molecular pathways for the transmission of several acquired characters. Ciliates have long provided model systems to study phenomena that bypass traditional modes of inheritance. RNA, normally thought of as a conduit in gene expression, displays a novel mode of action in ciliated protozoa. For example, maternal RNA templates provide both an organizing guide for DNA rearrangements in Oxytricha and a template that can transmit spontaneous mutations that may arise during somatic growth to the next generation, providing two such mechanisms of so-called Lamarckian inheritance. This suggests that the somatic ciliate genome is really an 'epigenome', formed through templates and signals arising from the previous generation. This review will discuss these new biological roles for RNA, including non-coding 'template' RNA molecules. The evolutionary consequences of viable mechanisms in ciliates to transmit acquired characters may create an additional store of heritable variation that contributes to the cosmopolitan success of this diverse lineage of microbial eukaryotes.

  10. Lentivirus vector-mediated gene transfer to the developing bronchiolar airway epithelium in the fetal lamb.

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    Yu, Ze-Yan; McKay, Karen; van Asperen, Peter; Zheng, Maolin; Fleming, Jane; Ginn, Samantha L; Kizana, Eddy; Latham, Margot; Feneley, Michael P; Kirkland, Peter D; Rowe, Peter B; Lumbers, Eugenie R; Alexander, Ian E

    2007-06-01

    Development of effective and durable gene therapy for treatment of the respiratory manifestations of cystic fibrosis remains a formidable challenge. Obstacles include difficulty in achieving efficient gene transfer to mature airway epithelium and the need to stably transduce self-renewing epithelial progenitor cells in order to avoid loss of transgene expression through epithelial turnover. Targeting the developing airway epithelium during fetal life offers the prospect of circumventing these challenges. In the current study we investigated vesicular stomatitis virus glycoprotein (VSVg)-pseudotyped HIV-1-derived lentivirus vector-mediated gene transfer to the airway epithelium of mid-gestation fetal lambs, both in vitro and in vivo. In the in vitro studies epithelial sheet explants and lung organ culture were used to examine transduction of the proximal and more distal airway epithelium, respectively. For the in vivo studies, vector was delivered directly into the proximal airway. We found that even during the early pseudoglandular and canalicular phases of lung development, occurring through mid-gestation, the proximal bronchial airway epithelium was relatively mature and highly resistant to lentivirus-mediated transduction. In contrast, the more distal bronchiolar airway epithelium was relatively permissive for transduction although the absolute levels achieved remained low. This result is promising as the bronchiolar airway epithelium is a major site of pathology in the cystic fibrosis airway, and much higher levels of transduction are likely to be achieved by developing strategies that increase the amount of vector reaching the more distal airway after intratracheal delivery.

  11. Functional diversity of aquatic ciliates.

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    Weisse, Thomas

    2017-10-01

    This paper first reviews the concept of functional diversity in general terms and then applies it to free-living aquatic ciliates. Ciliates are extremely versatile organisms and display an enormous functional diversity as key elements of pelagic food webs, acting as predators of bacteria, algae, other protists and even some metazoans. Planktonic ciliates are important food for zooplankton, and mixotrophic and functionally autotrophic species may significantly contribute to primary production in the ocean and in lakes. The co-occurrence of many ciliate species in seemingly homogenous environments indicates a wide range of their ecological niches. Variation in space and time may foster co-occurrence and prevent violating the competitive exclusion principle among ciliates using the same resources. Considering that many ciliates may be dormant and/or rare in many habitats, ciliate species diversity must be higher than can be deduced from simple sampling techniques; molecular methods of identification clearly point to this hidden diversity. From a functional point of view, the question is how much of this diversity represents redundancy. A key challenge for future research is to link the ecophysiological performance of naturally co-occurring ciliates to their functional genes. To this end, more experimental research is needed with with functionally different species. Copyright © 2017 The Author. Published by Elsevier GmbH.. All rights reserved.

  12. Ciliated Cells of Pseudostratified Airway Epithelium Do Not Become Mucous Cells after Ovalbumin Challenge

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    Pardo-Saganta, Ana; Law, Brandon M.; Gonzalez-Celeiro, Meryem; Vinarsky, Vladimir

    2013-01-01

    Mucous cell metaplasia is a hallmark of airway diseases, such as asthma and chronic obstructive pulmonary disease. The majority of human airway epithelium is pseudostratified, but the cell of origin of mucous cells has not been definitively established in this type of airway epithelium. There is evidence that ciliated, club cell (Clara), and basal cells can all give rise to mucus-producing cells in different contexts. Because pseudostratified airway epithelium contains distinct progenitor cells from simple columnar airway epithelium, the lineage relationships of progenitor cells to mucous cells may be different in these two epithelial types. We therefore performed lineage tracing of the ciliated cells of the murine basal cell–containing airway epithelium in conjunction with the ovalbumin (OVA)-induced murine model of allergic lung disease. We genetically labeled ciliated cells with enhanced Yellow Fluorescent Protein (eYFP) before the allergen challenge, and followed the fate of these cells to determine whether they gave rise to newly formed mucous cells. Although ciliated cells increased in number after the OVA challenge, the newly formed mucous cells were not labeled with the eYFP lineage tag. Even small numbers of labeled mucous cells could not be detected, implying that ciliated cells make virtually no contribution to the new goblet cell pool. This demonstrates that, after OVA challenge, new mucous cells do not originate from ciliated cells in a pseudostratified basal cell–containing airway epithelium. PMID:23239495

  13. CLARA: CLAS12 Reconstruction and Analysis Framework

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    Gyurjyan, Vardan [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Matta, Sebastian Mancilla [Santa Maria U., Valparaiso, Chile; Oyarzun, Ricardo [Santa Maria U., Valparaiso, Chile

    2016-11-01

    In this paper we present SOA based CLAS12 event Reconstruction and Analyses (CLARA) framework. CLARA design focus is on two main traits: real-time data stream processing, and service-oriented architecture (SOA) in a flow based programming (FBP) paradigm. Data driven and data centric architecture of CLARA presents an environment for developing agile, elastic, multilingual data processing applications. The CLARA framework presents solutions capable of processing large volumes of data interactively and substantially faster than batch systems.

  14. Local ciliate communities associated with aquatic macrophytes.

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    Yeates, Anna M; Esteban, Genoveva F

    2014-03-01

    This study, based within the catchment area of the River Frome, an important chalk stream in the south of England, compared ciliated protozoan communities associated with three species of aquatic macrophyte common to lotic habitats: Ranunculus penicillatus subsp. pseudofluitans, Nasturtium officinale and Sparganium emersum. A total of 77 ciliate species were counted. No species-specific ciliate assemblage was found to be typical of any one plant species. Ciliate abundance between plant species was determined to be significantly different. The ciliate communities from each plant species were unique in that the number of species increased with ciliate abundance. The community associated with R. penicillatus subsp. pseudofluitans showed the highest consistency and species richness whereas S. emersum ciliate communities were unstable. Most notably, N. officinale was associated with low ciliate abundances and an apparent reduction in biofilm formation, discussed herein in relation to the plant's production of the microbial toxin phenethyl isothiocyanate. We propose that the results reflect differences in the quantity and quality of biofilm present on the plants, which could be determined by the different plant morphologies, patterns of plant decay and herbivore defense systems, all of which suppress or promote the various conditions for biofilm growth.

  15. The trophic position of planktonic ciliate populations in the food ...

    African Journals Online (AJOL)

    Most predaceous ciliates were haptorids, and consumed other ciliates or metazoa. Growth rate estimates for planktonic ciliate populations in East African lakes ranged from 0.18 to 6.56d–1. From these growth rates and biomass, the estimated production of ciliate populations ranged from 5.9 to 2 335µg carbon l–1 d–1 and ...

  16. Clara Maass, yellow fever and human experimentation.

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    Chaves-Carballo, Enrique

    2013-05-01

    Clara Louise Maass, a 25-year-old American nurse, died of yellow fever on August 24, 1901, following experimental inoculation by infected mosquitoes in Havana, Cuba. The human yellow fever experiments were initially conducted by MAJ Walter Reed, who first used written informed consent and proved the validity of Finlay's mosquito-vector hypothesis. Despite informed consent form and an incentive of $100 in U.S. gold, human subjects were exposed to a deadly virus. The deaths of Clara Maass and two Spanish immigrants resulted in a public outcry and the immediate cessation of yellow fever human experiments in Cuba. Reprint & Copyright © 2013 Association of Military Surgeons of the U.S.

  17. Guilds of ciliate microzooplankton in the Chesapeake Bay

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    Dolan, John R.

    1991-08-01

    The composition and abundance of three major guilds, or different trophic groups, of ciliates are reported for the Chesapeake Bay. Ciliates were classified either (a) macrophagous (consumers of nanoplankton-size or larger prey), (b) microphagous (consumers of picoplankton-size prey), or (c) predatory (consumers of other ciliates). The three guilds show seasonal changes in species composition and distinct abundance trends, based on samples taken from April to October, 1985-1987. Macrophagous forms (mostly tintinnids and large oligotrichs) represent, on average, c. 73% of ciliate biomass. However, microphagous species (largely scuticociliates and small oligotrichs) are dominant in terms of cell numbers and often form a substantial portion of the ciliate biomass in deep waters; they probably account for c. 15% of total protozooplankton grazing pressure on bacteria in the Chesapeake Bay. Predatory ciliates, while numerically a minor component of total ciliate numbers, were correlated with microphagous ciliate biomass and may, at times, have had a major impact on the microphagous guild.

  18. 27 CFR 9.126 - Santa Clara Valley.

    Science.gov (United States)

    2010-04-01

    ...), photoinspected 1973; (8) Loma Prieta, Calif., 1955 (photorevised 1968); (9) Los Gatos, Calif., 1953 (photorevised... boundary) approx. 7 miles until it converges with Los Trancos Creek (Santa Clara County/San Mateo County... along Los Trancos Creek (Santa Clara County/San Mateo County boundary) until it intersects the 600 foot...

  19. Expression of Clara cell secretory protein in experimental otitis media in the rat.

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    Kim, Seo Jin; Jung, Hak Hyun

    2005-01-01

    These results suggest that CCSP is upregulated in OME and may play a protective role in the pathogenesis of OME. Clara cell secretory protein (CCSP) is an abundant 16-kDa homodimeric protein and is secreted by non-ciliated secretory epithelial cells in the lung. It has an important protective role against the intrapulmonary inflammatory process. The aim of this study was to investigate the expression of CCSP in endotoxin-induced otitis media with effusion (OME) in the rat. We instilled endotoxin and saline (control) into the middle ear cavity of the rat. Middle ear mucosa were taken at 0, 1, 3, 6 and 12 h and 1, 3, 7 and 14 days, and the expression of both CCSP mRNA and protein were then evaluated using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. RT-PCR revealed that the expression of CCSP was first identified at 1 h after endotoxin instillation, was dramatically increased between 1 h and Day 1, with maximal expression at 12 h, and then decreased after Day 3. The expression pattern of CCSP protein identified by means of Western blotting was similar to the CCSP mRNA patterns observed using RT-PCR. Expression of CCSP at both mRNA and protein levels was not detected in either normal middle ear mucosa or saline-instilled middle ear mucosa. Immunohistochemistry revealed that some epithelial cells in the middle ear mucosa were stained.

  20. A plasma wakefield acceleration experiment using CLARA beam

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    Xia, G., E-mail: guoxing.xia@cockcroft.ac.uk [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); Angal-Kalinin, D.; Clarke, J. [STFC/ASTeC, Daresbury, Warrington (United Kingdom); Smith, J. [Tech-X UK Corporation, Daresbury Innovation Centre, Warrington (United Kingdom); Cormier-Michel, E. [Tech-X Corporation, Boulder, CO (United States); Jones, J.; Williams, P.H.; Mckenzie, J.W.; Militsyn, B.L. [STFC/ASTeC, Daresbury, Warrington (United Kingdom); Hanahoe, K.; Mete, O. [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); Aimidula, A.; Welsch, C.P. [The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); The University of Liverpool, Liverpool (United Kingdom)

    2014-03-11

    We propose a Plasma Accelerator Research Station (PARS) based at proposed FEL test facility CLARA (Compact Linear Accelerator for Research and Applications) at Daresbury Laboratory. The idea is to use the relativistic electron beam from CLARA, to investigate some key issues in electron beam transport and in electron beam driven plasma wakefield acceleration, e.g. high gradient plasma wakefield excitation driven by a relativistic electron bunch, two bunch experiment for CLARA beam energy doubling, high transformer ratio, long bunch self-modulation and some other advanced beam dynamics issues. This paper presents the feasibility studies of electron beam transport to meet the requirements for beam driven wakefield acceleration and presents the plasma wakefield simulation results based on CLARA beam parameters. Other possible experiments which can be conducted at the PARS beam line are also discussed.

  1. Ciliated Protozoa of the polluted Tees estuary

    Science.gov (United States)

    Parker, James G.

    1981-03-01

    In a study of the ciliated Protozoa of Bran Sand, a sheltered beach in the Tees estuary, 20 putative species were identified. This beach was richer in species than the nearby North Gare beach. In experimental batch cultures, seawater from the estuary had an inhibitory effect upon growth of a strain of Uronema marinum Dujardin which was isolated from an unpolluted beach at Robin Hood's Bay. The tolerance to metals of a Tees strain of U. marinum was assessed in simple toxicity tests; lethal levels for this strain were found to be similar to those reported elsewhere for the Robin Hood's Bay strain.

  2. Characterization of copper resistant ciliates: Potential candidates for ...

    African Journals Online (AJOL)

    Characterization of copper resistant ciliates: Potential candidates for consortia of organisms used in bioremediation of wastewater. ... Copper is one of such contaminant found in the wastewater of local industries. ... Key words: Copper toxicity, metallothionein, growth curve of ciliates, metal uptake, bioremediation.

  3. Feeding of the tintinnid ciliate Favella taraikaensis on the toxic ...

    African Journals Online (AJOL)

    To establish the fate of paralytic shellfish poisoning (PSP) toxins in planktonic foodwebs, feeding activities of the planktonic ciliate Favella taraikaensis on the PSP-producing alga Alexandrium tamarense were examined in laboratory experiments and the toxin content in the ciliates was quantified by fluorometric ...

  4. Vitamin A and ciliated cells. I. Respiratory epithelia.

    Science.gov (United States)

    Biesalski, H K; Stofft, E; Wellner, U; Niederauer, U; Bässler, K H

    1986-06-01

    To estimate the role of vitamin A on ciliated cells we investigated whether ciliated cells undergo any alteration during vitamin A deficiency. The epithelia examined include the ciliated cells of the respiratory tract and the ciliated sensory cells of the inner ear, the tongue, and the olfactory cells. This part of the paper will describe the ciliated epithelium of the tracheobronchial tract and its relation to vitamin A status. During vitamin A deficiency a partial loss of ciliae can be observed before any squamous metaplasia (which usually occurs during longer lasting vitamin A deficiency) develops. The scanning electron microscopic data illustrate the altered surface of the epithelium during vitamin A deficiency better than transmission electron microscopy.

  5. En el Cincuentenario del Hospital Santa Clara

    Directory of Open Access Journals (Sweden)

    Gilberto Rueda Pérez

    1992-12-01

    Full Text Available

    El 16 de julio de 1942 nace el Hospital Sanatorio Antituberculoso Santa Clara en Santafé de Bogotá.
    Un siglo atrás, casi a la fecha, en 1843, nace en Alemania Roberto Koch, quien, 60 años antes de la fundación del Hospital, el 24 de marzo de 1882, presenta al mundo el descubrimiento del Micobacterium Tuberculosis, germen causante de la enfermedad que ataca alhombre desde sus más remotos orígenes y que aún nos acompaña, especialmente en los países subdesarrollados, causando severo impacto en la salud de las poblaciones más necesitadas, apesar de los grandes avances alcanzados en el campo de la Medicina a nivel mundial.
    Por haber sido destinado el Hospital altratamiento de los tuberculosos, destino que aún conserva primordialmente aunque, como se verá posteriormente, sus camas reciben enfermos de medicina general dada la evolución de los tratamientos y la modernización de los esquemas terapéuticos, se justifica ampliamente mencionar aquí los principales avances relacionados con el control de la Tuberculosis a través de los tiempos hasta la fundación del Hospital.

    Estos se pueden resumir así:

    • 'HIPOCRATES (460-377 a. C.:describe la consunción y la llama tisis; lanza el concepto de herencia que perdura por siglos.
    • ARISTOTELES(324-284a. C.:habla del contagio a través de la respiración.
    • CELSO (siglo I a. C.: describe el tubérculo y señala tres formas de consunción: atrofia, caquexia y tisis.
    • GALENO(181-261 d. C.: la agrupa con otras enfermedades transmisibles: la peste, la sarna, etc.
    • EDADMEDIA(sigloVII alXIIId. C.:se destaca únicamente como aporte nuevo Maimonides, filósofo judío radicado en Granada (11351204,quien describe la tisis de los animales.

    Posteriormente Girolamo Fracastoro (1478-1553, nacido en Verona,la asimila a la viruela y lanza la teoría microbiana.

    • PARACELSO(1493-1541pregona que: los

    • Development of A Clara Service for Neutron Reconstruction

      Science.gov (United States)

      Carbonneau, Joshua; Moog, Mark; Gilfoyle, Gerard; Gyurjyan, Vardan; Paul, Sebouh; Jefferson Lab Collaboration

      2011-10-01

      The Clara framework is currently being used by Jefferson Lab to reconstruct simulated events from CLAS12, a large particle detector being built as part of the Jefferson Laboratory 12 GeV Upgrade. Clara is a software package that allows for large programming tasks to be split into components and distributed among different computers. Clara takes full advantage of the multi-core, multi-threaded capabilities of modern computers, allowing for greater efficiency when processing large data sets. These components, called services, are language-independent, and communication between them is implemented as a publish/subscribe architecture. When data is fed to a service, it processes the data and then ``publishes'' the result, allowing all services that have ``subscribed'' to the output to receive the data. Applications can be built from already existing services to meet a user's need. A neutron reconstruction algorithm has been developed as a C++ service that extends the existing electron reconstruction algorithm.

    • SPECIES COMPOSITION OF PSAMMOPHILIC CILIATES OF SUMGAIT CASPIAN COAST

      Directory of Open Access Journals (Sweden)

      I. Kh. Alekperov

      2014-01-01

      Full Text Available Abstract. Comparative data on the species composition and species saprobic indicators of the psammon ciliates from Sumgait coast of the Caspian Sea are given.Methods. “Alive” samples were collected using a small clean plastic wide mouth bottles. Further processing was carried out under laboratory conditions. Small quantities of soil were examined under a binocular microscope MBS-9 . Ciliates detected microvessel caught and fixed castors for further impregnation kinetoma silver nitrate (Chatton et Lwoff, 1930 or silver proteinate. To determine the keys for ciliates used Foyssner’s major publications (Foissner et al., 1991, 1992, 1999 and “Free-living ciliates Atlas” (Alekperov, 2005.Results. We observed 75 species of ciliates during the studies, which species composition and distribution of the collection points are shown in table 1. Diagrams with average data were made relations groups psammophilous ciliates indicators saprobity different zones for each of the sites investigated Sumgait coast.Main conclusions. Environmental analysis using benthic ciliates indicators saprobity different zones showed that as expected, the industrial zone of Sumgayit coast coast, despite the decline in recent years, the total amount of pollution that is still highly contaminated portion of the coastal zone of the Caspian Sea.

    • Plasma wakefield acceleration at CLARA facility in Daresbury Laboratory

      Energy Technology Data Exchange (ETDEWEB)

      Xia, G., E-mail: guoxing.xia@manchester.ac.uk [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); Nie, Y. [Deutsche Elektronen-Synchrotron DESY, Hamburg (Germany); Mete, O.; Hanahoe, K. [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); Dover, M.; Wigram, M.; Wright, J.; Zhang, J. [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); Smith, J. [Tech-X UK Corporation, Daresbury Innovation Centre, Warrington (United Kingdom); Pacey, T.; Li, Y. [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom); The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); Wei, Y.; Welsch, C. [The Cockcroft Institute, Sci-Tech Daresbury, Daresbury, Warrington (United Kingdom); University of Liverpool, Liverpool (United Kingdom)

      2016-09-01

      A plasma accelerator research station (PARS) has been proposed to study the key issues in electron driven plasma wakefield acceleration at CLARA facility in Daresbury Laboratory. In this paper, the quasi-nonlinear regime of beam driven plasma wakefield acceleration is analysed. The wakefield excited by various CLARA beam settings are simulated by using a 2D particle-in-cell (PIC) code. For a single drive beam, an accelerating gradient up to 3 GV/m can be achieved. For a two bunch acceleration scenario, simulation shows that a witness bunch can achieve a significant energy gain in a 10–50 cm long plasma cell.

    • Experimental and modeling evidence of appendicularian-ciliate interactions

      DEFF Research Database (Denmark)

      Lombard, Fabien; Eloire, D.; Gobet, A.

      occurred simultaneously, resulting in apparent mutualism. The large ciliate Strombidium sp. entered the inlet filters of appendicularian houses (larger than 500 µm body size) by distorting the mesh. Once inside, Strombidium fed on particles concentrated on the filters. When appendicularians were larger...... than 900 µm, both the high flow rate in the buccal tube and their esophagus width allowed the "host" appendicularian to capture and ingest ciliates. Thus, ciliates seem to be sequentially competitors, then parasites or commensal in appendicularian houses, and finally prey for appendicularians...

    • Experimental and modeling evidence of appendicularian-ciliate interactions

      DEFF Research Database (Denmark)

      Lombard, Fabien; Eloire, Damien; Gobet, Angelique

      2010-01-01

      occurred simultaneously, resulting in apparent mutualism. The large ciliate Strombidium sp. entered the inlet filters of appendicularian houses (larger than 500 mu m body size) by distorting the mesh. Once inside, Strombidium fed on particles concentrated on the filters. When appendicularians were larger...... than 900 mu m, both the high flow rate in the buccal tube and their esophagus width allowed the "host'' appendicularian to capture and ingest ciliates. Thus, ciliates seem to be sequentially competitors, then parasites or commensal in appendicularian houses, and finally prey for appendicularians...

    • Coyote Creek (Santa Clara County) Pilot Revegetation Project

      Science.gov (United States)

      John T. Stanley; L. R. Silva; H. C. Appleton; M. S. Marangio; W. J. Lapaz; B. H. Goldner

      1989-01-01

      The Santa Clara Valley Water District, located in Northern California, is currently evaluating a pilot riparian revegetation project on a 1.6 ha (4 ac) site adjacent to Coyote Creek in the south San Francisco Bay Area. Specific techniques used during the design, site preparation and installation of 3640 plants (including seed planting locations) are described. This...

    • Natural Law, Santa Clara, and the Supreme Court.

      Science.gov (United States)

      Rodgers, Raymond S.; Lujan, Phillip

      The court case, "Santa Clara Pueblo, et al. v. Julia Martinez, et al.," is the subject of this paper. It gives the background of the case of a woman whose children were refused admittance to tribal rolls because of an ordinance prohibiting the enrollment of children whose father is not a tribal member. The paper gives the arguments of…

    • The effect of salinity on some endocommensalic ciliates from shipworms

      Digital Repository Service at National Institute of Oceanography (India)

      Santhakumari, V.

      The ciliates, Nucleocorbula adherens, Boveria teredinidi, Trichodina balakrishnia, Thigmozoon fencheli and Nyctothereus marina, live inside the mantle cavity of the ship-worms in the estuaries and backwaters of the south-west coast of India...

    • A Holotrichous ciliate from the coelom of Chaetognaths

      Digital Repository Service at National Institute of Oceanography (India)

      Santhakumari, V.

      A holotrichous ciliate was observed from the body cavity of Chaetognaths Sagitta enflata and S. bedoti collected from the zooplankton samples at Cape Comorin and Tuticorin (Tamil Nadu, India). Species were examined for infected parasites...

    • Studies on experimental culture of a marine ciliate Fabrea salina

      Digital Repository Service at National Institute of Oceanography (India)

      Rattan, R.; Ansari, Z.A.; Chatterji, A.

      Studies were conducted on the culture of a marine ciliate, Fabrea salina in the laboratory condition. Three types of inert feed; commercial yeast, fermented wheat bran and fermented rise bran were tested to study their suitability as artificial feed...

    • DNA rearrangements directed by non-coding RNAs in ciliates

      OpenAIRE

      Mochizuki, Kazufumi

      2010-01-01

      Extensive programmed rearrangement of DNA, including DNA elimination, chromosome fragmentation, and DNA descrambling, takes place in the newly developed macronucleus during the sexual reproduction of ciliated protozoa. Recent studies have revealed that two distant classes of ciliates use distinct types of non-coding RNAs to regulate such DNA rearrangement events. DNA elimination in Tetrahymena is regulated by small non-coding RNAs that are produced and utilized in an RNAi-related process. It ...

    • Free-living ciliates from epiphytic tank bromeliads in Mexico.

      Science.gov (United States)

      Durán-Ramírez, Carlos Alberto; García-Franco, José Guadalupe; Foissner, Wilhelm; Mayén-Estrada, Rosaura

      2015-02-01

      The ciliate diversity of Mexican bromeliads is poorly known. We studied the ciliate community of two species of epiphytic tank bromeliads from 48 individuals of Tillandsia heterophylla and four of T. prodigiosa. The bromeliads occurred on over 22 tree host species. Samples were collected during 2009 and 2010 in a mountain cloud forest and in two coffee plantations and in a pine-oak forest. The ciliates were identified in live and protargol preparations. We recorded 61 ciliate species distributed in 39 genera grouped in eight classes. Ten species were frequent in the 52 samples (20 ± 3.2) and Leptopharynx bromeliophilus was the most frequent recorded in 25 samples. Thirty-three species are new for the fauna of Mexico, 24 species have been recorded for the first time in tank bromeliads. The classes Spirotrichea, Oligohymenophorea and Colpodea presented the highest number of species, 16, 14, and 12, respectively. Colpoda was the most species-rich genus being present with six species. A low similarity between areas and seasons was obtained with Jaccard's index. We conclude that the two bromeliads species host a rich ciliate diversity whose knowledge contributes to the question of ciliate distribution and specifically, in tank bromeliads. Copyright © 2014 Elsevier GmbH. All rights reserved.

    • Investigating the biodiversity of ciliates in the 'Age of Integration'.

      Science.gov (United States)

      Clamp, John C; Lynn, Denis H

      2017-10-01

      Biology is now turning toward a more integrative approach to research, distinguished by projects that depend on collaboration across hierarchical levels of organization or across disciplines. This trend is prompted by the need to solve complex, large-scale problems and includes disciplines that could be defined as integrative biodiversity. Integrative biodiversity of protists, including that of ciliates, is still partially in its infancy. This is the result of a shortage of historical data resources such as curated museum collections. Major areas of integrative biodiversity of ciliates that have begun to emerge can be categorized as integrative systematics, phenotypic plasticity, and integrative ecology. Integrative systematics of ciliates is characterized by inclusion of diverse sources of data in treatment of taxonomy of species and phylogenetic investigations. Integrative research in phenotypic plasticity combines investigation of functional roles of individual species of ciliates with genetic and genomic data. Finally, integrative ecology focuses on genetic identity of species in communities of ciliates and their collective functional roles in ecosystems. A review of current efforts toward integrative research into biodiversity of ciliates reveals a single, overarching concern-rapid progress will be achieved only by implementing a comprehensive strategy supported by one or more groups of active researchers. Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.

    • Network of interactions between ciliates and phytoplankton during spring

      Directory of Open Access Journals (Sweden)

      Thomas ePosch

      2015-11-01

      Full Text Available The annually recurrent spring phytoplankton blooms in freshwater lakes initiate pronounced successions of planktonic ciliate species. Although there is considerable knowledge on the taxonomic diversity of these ciliates, their species-specific interactions with other microorganisms are still not well understood. Here we present the succession patterns of 20 morphotypes of ciliates during spring in Lake Zurich, Switzerland, and we relate their abundances to phytoplankton genera, flagellates, heterotrophic bacteria, and abiotic parameters. Interspecific relationships were analyzed by contemporaneous correlations and time-lagged co-occurrence and visualized as association networks. The contemporaneous network pointed to the pivotal role of distinct ciliate species (e.g., Balanion planctonicum, Rimostrombidium humile as primary consumers of cryptomonads, revealed a clear overclustering of mixotrophic / omnivorous species, and highlighted the role of Halteria / Pelagohalteria as important bacterivores. By contrast, time-lagged statistical approaches (like local similarity analyses, LSA proved to be inadequate for the evaluation of high-frequency sampling data. LSA led to a conspicuous inflation of significant associations, making it difficult to establish ecologically plausible interactions between ciliates and other microorganisms. Nevertheless, if adequate statistical procedures are selected, association networks can be powerful tools to formulate testable hypotheses about the autecology of only recently described ciliate species.

    • The symbiotic intestinal ciliates and the evolution of their hosts.

      Science.gov (United States)

      Moon-van der Staay, Seung Yeo; van der Staay, Georg W M; Michalowski, Tadeusz; Jouany, Jean-Pierre; Pristas, Peter; Javorský, Peter; Kišidayová, Svetlana; Varadyova, Zora; McEwan, Neil R; Newbold, C Jamie; van Alen, Theo; de Graaf, Rob; Schmid, Markus; Huynen, Martijn A; Hackstein, Johannes H P

      2014-04-01

      The evolution of sophisticated differentiations of the gastro-intestinal tract enabled herbivorous mammals to digest dietary cellulose and hemicellulose with the aid of a complex anaerobic microbiota. Distinctive symbiotic ciliates, which are unique to this habitat, are the largest representatives of this microbial community. Analyses of a total of 484 different 18S rRNA genes show that extremely complex, but related ciliate communities can occur in the rumen of cattle, sheep, goats and red deer (301 sequences). The communities in the hindgut of equids (Equus caballus, Equus quagga), and elephants (Elephas maximus, Loxodonta africanus; 162 sequences), which are clearly distinct from the ruminant ciliate biota, exhibit a much higher diversity than anticipated on the basis of their morphology. All these ciliates from the gastro-intestinal tract constitute a monophyletic group, which consists of two major taxa, i.e. Vestibuliferida and Entodiniomorphida. The ciliates from the evolutionarily older hindgut fermenters exhibit a clustering that is specific for higher taxa of their hosts, as extant species of horse and zebra on the one hand, and Africa and Indian elephant on the other hand, share related ciliates. The evolutionary younger ruminants altogether share the various entodiniomorphs and the vestibuliferids from ruminants. Copyright © 2014 Elsevier GmbH. All rights reserved.

  1. Inhibition of the Rumen Ciliate Entodinium caudatum by Antibiotics.

    Science.gov (United States)

    Park, Tansol; Meulia, Tea; Firkins, Jeffrey L; Yu, Zhongtang

    2017-01-01

    Axenic cultures of free-living aerobic ciliates, such as Tetrahymena thermophila and Paramecium aurelia, have been established and routinely used in laboratory research, greatly facilitating, or enabling characterization of their metabolism, physiology, and ecology. Ruminal protozoa are anaerobic ciliates, and they play important roles in feed digestion and fermentation. Although, repeatedly attempted, no laboratory-maintainable axenic culture of ruminal ciliates has been established. When axenic ciliate cultures are developed, antibiotics are required to eliminate the accompanying bacteria. Ruminal ciliates gradually lose viability upon antibiotic treatments, and the resultant axenic cultures can only last for short periods of time. The objective of this study was to evaluate eight antibiotics that have been evaluated in developing axenic cultures of ruminal ciliates, for their toxicity to Entodinium caudatum, which is the most predominant ruminal ciliate species. Scanning and transmission electron microscopy (TEM) showed that the antibiotics damaged both the cell surface and nuclei of E. caudatum and increased accumulation of intracellular glycogen. Combinations of the three least toxic antibiotics failed to eliminate the bacteria that are present in the E. caudatum culture. The combination of ampicillin, carbenicillin, streptomycin, and oxytetracycline was able to eliminate all the bacteria, but the resultant axenic E. caudatum culture gradually lost viability. Adding the bacterial fraction (live) separated from an untreated E. caudatum culture reversed the viability decline and recovered the growth of the treated E. caudatum culture, whereas feeding nine strains of live bacteria isolated from E. caudatum cells, either individually or in combination, could not. Nutritional and metabolic dependence on its associated bacteria, accompanied with direct and indirect inhibition by antibiotics, makes it difficult to establish an axenic culture of E. caudatum. Monoxenic

  2. Inhibition of the Rumen Ciliate Entodinium caudatum by Antibiotics

    Directory of Open Access Journals (Sweden)

    Tansol Park

    2017-06-01

    Full Text Available Axenic cultures of free-living aerobic ciliates, such as Tetrahymena thermophila and Paramecium aurelia, have been established and routinely used in laboratory research, greatly facilitating, or enabling characterization of their metabolism, physiology, and ecology. Ruminal protozoa are anaerobic ciliates, and they play important roles in feed digestion and fermentation. Although, repeatedly attempted, no laboratory-maintainable axenic culture of ruminal ciliates has been established. When axenic ciliate cultures are developed, antibiotics are required to eliminate the accompanying bacteria. Ruminal ciliates gradually lose viability upon antibiotic treatments, and the resultant axenic cultures can only last for short periods of time. The objective of this study was to evaluate eight antibiotics that have been evaluated in developing axenic cultures of ruminal ciliates, for their toxicity to Entodinium caudatum, which is the most predominant ruminal ciliate species. Scanning and transmission electron microscopy (TEM showed that the antibiotics damaged both the cell surface and nuclei of E. caudatum and increased accumulation of intracellular glycogen. Combinations of the three least toxic antibiotics failed to eliminate the bacteria that are present in the E. caudatum culture. The combination of ampicillin, carbenicillin, streptomycin, and oxytetracycline was able to eliminate all the bacteria, but the resultant axenic E. caudatum culture gradually lost viability. Adding the bacterial fraction (live separated from an untreated E. caudatum culture reversed the viability decline and recovered the growth of the treated E. caudatum culture, whereas feeding nine strains of live bacteria isolated from E. caudatum cells, either individually or in combination, could not. Nutritional and metabolic dependence on its associated bacteria, accompanied with direct and indirect inhibition by antibiotics, makes it difficult to establish an axenic culture of E

  3. Status of Santa Clara MCFC product development test

    Energy Technology Data Exchange (ETDEWEB)

    Leo, A.J.; O`Shea, T.P.

    1995-08-01

    The objective of the 2MW Santa Clara Demonstration Project is the demonstration of the carbonate fuel cell technology at full scale. Additional objectives of the project include the demonstration of specific advantages of the direct carbonate fuel cell power plant, such as high efficiency, low emissions, reactive power capability, and high reliability and availability. The project will also provide design input for precommercial early production power plants.

  4. Santa Clara County Planar Solid Oxide Fuel Cell Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Fred Mitlitsky; Sara Mulhauser; David Chien; Deepak Shukla; David Weingaertner

    2009-11-14

    The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project demonstrated the technical viability of pre-commercial PSOFC technology at the County 911 Communications headquarters, as well as the input fuel flexibility of the PSOFC. PSOFC operation was demonstrated on natural gas and denatured ethanol. The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project goals were to acquire, site, and demonstrate the technical viability of a pre-commercial PSOFC technology at the County 911 Communications headquarters. Additional goals included educating local permit approval authorities, and other governmental entities about PSOFC technology, existing fuel cell standards and specific code requirements. The project demonstrated the Bloom Energy (BE) PSOFC technology in grid parallel mode, delivering a minimum 15 kW over 8760 operational hours. The PSOFC system demonstrated greater than 81% electricity availability and 41% electrical efficiency (LHV net AC), providing reliable, stable power to a critical, sensitive 911 communications system that serves geographical boundaries of the entire Santa Clara County. The project also demonstrated input fuel flexibility. BE developed and demonstrated the capability to run its prototype PSOFC system on ethanol. BE designed the hardware necessary to deliver ethanol into its existing PSOFC system. Operational parameters were determined for running the system on ethanol, natural gas (NG), and a combination of both. Required modeling was performed to determine viable operational regimes and regimes where coking could occur.

  5. The role of ciliate protozoa in the rumen

    Directory of Open Access Journals (Sweden)

    Charles James Newbold

    2015-11-01

    Full Text Available First described in 1843, Rumen protozoa with their striking appearance were assumed to be important for the welfare of their host. However, despite contributing up to 50% of the bio-mass in the rumen, the role of protozoa in rumen microbial ecosystem remains unclear.Phylogenetic analysis of 18S rDNA libraries generated from the rumen of cattle, sheep, and goats has revealed an unexpected diversity of ciliated protozoa although variation in gene copy number between species makes it difficult to obtain absolute quantification. Despite repeated attempts it has proven impossible to maintain rumen protozoa in axenic culture. Thus it has been difficult to establish conclusively a role of ciliate protozoa in rumen fibre degradation. The development of techniques to clone and express ciliate genes in  phage, together with bioinformatic indices to confirm the ciliate origin of the genes has allowed the isolation and characterisation of fibrolytic genes from rumen protozoa. Elimination of the ciliate protozoa increases microbial protein supply by up to 30% and reduces methane production by up to 11%. Our recent findings suggest that holotrich protozoa play a disproportionate role in supporting methanogenesis whilst the small entodiniium are responsible for much of the bacterial protein turnover. As yet no method to control protozoa in the rumen that is safe and practically applicable has been developed, however a range of plant extract capable of controlling if not completely eliminating rumen protozoa have been described.

  6. Planktonic ciliates in a hypertrophic pond: functional role and importance.

    Science.gov (United States)

    Sanchez Rodriguez, Ma del Rosario; Lugo Vazquez, Alfonso; Oliva Martinez, Ma Guadalupe; Verver y Vargas, Jaqueline Garcia; Rodriguez Rocha, Armando; Peralta Soriano, Laura

    2011-07-01

    Taxonomical composition and abundance of the planktonic ciliate assemblage in a Lake Tezozomoc, a hypertrophic pond located into an urban park in Mexico City, was investigated along the rainy season (May to October). The aims of the study were to know the main trophic roles and ecological significance of ciliates in a highly productive environment. A low number of taxa (27) and a wide abundance fluctuation (104-387 cil ml(-1)) were found. The most abundant species (up to 162 cil ml(-1)) was Halteria grandinella, an oligotrich ciliate that graze on bacteria and picoplankton, but also several big body sized species that feed on pico and nanoplankton were abundant. Sudden temporal changes in species dominance occurred. Ciliate biomass was very high and fluctuated widely (1.6-88 10(6) microm(3) ml(-1)) being dominated by the >50 microm size fraction that mainly included the pico and nanoplankton feeders. Ciliates are a very important component in the plankton of hypertrophic lakes and their main control factor seems to be the grazing by big-body size Daphnia species.

  7. Bacterial endocytobionts of the ciliate Paramecium calkinsi.

    Science.gov (United States)

    Fokin, S; Sabaneyeva, E

    1993-11-12

    Five species of gram-negative bacteria inhabiting the cytoplasm and the macronucleus of the ciliate Paramecium calkinsi are described. Two species of endocytobionts have been found in the cytoplasm. Both of them were represented by only a vegetative form. In the case of the first species, numerous endocytobiotic bacteria (length, 2 μm; diameter, 0.3-0.4 μm) were enclosed in individual endobiontophoral vacuoles. The second one was not as abundant in its host cells and the bacteria lie freely in the host cytoplasm. These bacteria (length, 2-3 μm; diameter, 0.6-0.9 μm) often contained viral particles. Three species of endocytobionts have been discovered in the macronucleus. The first one (1-2 μm long, 0.3-0.4 μm in diameter) resembled Nonospora macronucleata. However, it differed from N. macronucleata in having specific electron dense zones in its cytoplasm and stained differently by Ag-NOR. The second one represents a new species of the Holospora genus: H. curvata sp. nov. The infectious form of this endocytobiont (15-20 μm long, 0.7-0.9 μm in diameter) is curved, the vegetative form (2-3 μm long, the same diameter) was rod-like or slightly curved. The host specificity of these endocytobionts is well expressed. The third species of intranuclear bacteria, H. bacillata, had been previously described in P. woodruffi. The infective capacity of H. curvata and two "stocks" of H. bacillata from different hosts was compared. Copyright © 1993 Gustav Fischer Verlag · Stuttgart · Jena · New York. Published by Elsevier GmbH.. All rights reserved.

  8. The Tara Oceans voyage reveals global diversity and distribution patterns of marine planktonic ciliates

    Science.gov (United States)

    Gimmler, Anna; Korn, Ralf; de Vargas, Colomban; Audic, Stéphane; Stoeck, Thorsten

    2016-01-01

    Illumina reads of the SSU-rDNA-V9 region obtained from the circumglobal Tara Oceans expedition allow the investigation of protistan plankton diversity patterns on a global scale. We analyzed 6,137,350 V9-amplicons from ocean surface waters and the deep chlorophyll maximum, which were taxonomically assigned to the phylum Ciliophora. For open ocean samples global planktonic ciliate diversity is relatively low (ca. 1,300 observed and predicted ciliate OTUs). We found that 17% of all detected ciliate OTUs occurred in all oceanic regions under study. On average, local ciliate OTU richness represented 27% of the global ciliate OTU richness, indicating that a large proportion of ciliates is widely distributed. Yet, more than half of these OTUs shared oceanic carbonate system and temperature. Planktonic ciliates displayed distinct vertical distributions relative to chlorophyll a. In contrast, the Tara Oceans dataset did not reveal any evidence that latitude is structuring ciliate communities. PMID:27633177

  9. Evaluation of lugol solution used for counting rumen ciliates

    Directory of Open Access Journals (Sweden)

    Marta D'Agosto

    1999-01-01

    Full Text Available The present study proposes a modification of the technique described by PURSER & MOIR (1959 for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984. The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.

  10. Clara E. Lida. Caleidoscopio del exilio. Actores, memoria, identidades

    Directory of Open Access Journals (Sweden)

    Rose Duroux

    2010-07-01

    Full Text Available Desde mediados de la década de 1980 he publicado y disertado sobre el exilio español en México en diversos foros académicos. Ello me ha permitido explorar distintas facetas vinculadas con el tema y recibir comentarios y sugerencias valiosas. De alguna manera, con la suma de todas estas miradas se construyeron los fragmentos que, como en los giros de un caleidoscopio, combinan reflexiones e imágenes a la vez complementarias y nuevas.Clara E. Lida Soixante-dix ans après la fin de la Guerre d’Es...

  11. TREATMENT SYSTEM FOR WASTEWATER AT VILLA CLARA WATER MANAGEMENT COMPANY

    Directory of Open Access Journals (Sweden)

    Floramis Pérez Martín

    2016-04-01

    Full Text Available The aim of this paper is to assess the current operating and safety conditions of biological treatment systems for wastewater in the centers of swinish and poultry breeding at Villa Clara Water Management Company, with the purpose of setting a group of organizational, technical and human measures that contributes to prevent contamination and minimize biological risks. In this way it can be guaranteed the protection to the workers, the facilities, community and the environment, to have a sure occupational atmosphere in the organization. As a result of the evaluation the factors that affect the operation of the biodigestion system and the security of the process are defined.

  12. Ciliated hepatic foregut cyst: a rare cystic liver lesion

    African Journals Online (AJOL)

    Adele

    Ciliated hepatic foregut cysts (CHFC) are rare congenital cystic lesions of the liver. CHFC are usually asymptomatic but may present with vague abdominal symptoms. CHFC are clinically important because of the possibility of malignant transformation1 and the diagnostic difficulties CHFC pose. We report the details of a ...

  13. CLARA: A Contemporary Approach to Physics Data Processing

    Energy Technology Data Exchange (ETDEWEB)

    V Gyurjyan, D Abbott, J Carbonneau, G Gilfoyle, D Heddle, G Heyes, S Paul, C Timmer, D Weygand, E Wolin

    2011-12-01

    In traditional physics data processing (PDP) systems, data location is static and is accessed by analysis applications. In comparison, CLARA (CLAS12 Reconstruction and Analysis framework) is an environment where data processing algorithms filter continuously flowing data. In CLARA's domain of loosely coupled services, data is not stored, but rather flows from one service to another, mutating constantly along the way. Agents, performing event processing, can then subscribe to particular data/events at any stage of the data transformation, and make intricate decisions (e.g. particle ID) by correlating events from multiple, parallel data streams and/or services. This paper presents a PDP application development framework based on service oriented and event driven architectures. This system allows users to design (Java, C++, and Python languages are supported) and deploy data processing services, as well as dynamically compose PDP applications using available services. The PDP service bus provides a layer on top of a distributed pub-sub middleware implementation, which allows complex service composition and integration without writing code. Examples of service creation and deployment, along with the CLAS12 track reconstruction application design will be presented.

  14. Letalidad por accidentes de trabajo en Villa Clara

    Directory of Open Access Journals (Sweden)

    Miguel Gómez Vital

    1999-01-01

    Full Text Available Se valora la problemática de la letalidad por accidentes laborales en la provincia de Villa Clara, en el período comprendido entre 1987 y 1997. Se determinan las principales causas, y fue la conducta negligente del individuo la que más incidió. En los últimos años de la década del 80 se registraron más cantidad de muertes por accidentes de trabajo, los que ocurrieron fundamentalmente en el puesto laboral. Queda puntualizado el importante rol del equipo médico en instituciones laborales.The problem of letality from working accidents in the province of Villa Clara from 1987 to 1997 was evaluated. The main causes were also determined. Individual negligent behaviour proved to be the first cause. More deaths from working accidents were registered late in the 1980s. Most of these accidents occurred at the working place. It was stressed the important role played by the medical team at the working institutions

  15. Fecal Ciliate Composition of Domestic Horses (Equus caballus Linnaeus, 1758) Living in Kyrgyzstan.

    Science.gov (United States)

    Gürelli, Gözde; Canbulat, Savaş; Aldayarov, Nurbek

    2015-11-03

    Species composition and distribution of intestinal ciliates were investigated in the feces from 15 domestic horses living in Bishkek, Kyrgyzstan. Twenty-three species belonging to 14 genera were identified. This is the first study on intestinal ciliates in domestic horses living in Kyrgyzstan. The mean number of ciliates was 14.1 ± 6.8 x10(4) cells ml(-1) of feces and the mean number of ciliate species per host was 6.0 ± 3.2. No endemic or new species were detected. Blepharocorys was the major genus as these ciliates were detected in high proportions. In contrast Holophryoides, Allantosoma were only observed at low frequencies. Recorded ciliate species in this investigation had almost the same characteristics as those described in previous studies. There was no important geographic variation in the intestinal ciliate fauna of equids.

  16. Intestinal ciliate composition found in the feces of the Cypriot wild donkey, Equus asinus Linnaeus, 1758.

    Science.gov (United States)

    Gürelli, Gözde; Göçmen, Bayram

    2010-01-01

    Species composition and distribution of large intestinal ciliates was investigated in the feces from 13 Cypriot wild donkeys, free-living in the Karpaz national park, Northern Cyprus. We identified 16 ciliate genera and 22 species. This is the first report on intestinal ciliates in Cypriot wild donkeys, and no endemic species were observed. The genus Cycloposthium occurred in all animals. The mean number of ciliates was 3.0+/-2.5 x 10(4) cells ml(-1) of feces and the mean number of ciliate species per host was 6.5+/-4.8. Characteristics of the wild donkey ciliates was almost identical to those reported in other equids from various regions around the world. We thus conclude that there is no pronounced geographic variation in the intestinal ciliate fauna of equids. Copyright (c) 2009 Elsevier GmbH. All rights reserved.

  17. Hydrogeologic framework of the Santa Clara Valley, California

    Science.gov (United States)

    Hanson, Randall T.

    2015-01-01

    The hydrologic framework of the Santa Clara Valley in northern California was redefined on the basis of new data and a new hydrologic model. The regional groundwater flow systems can be subdivided into upper-aquifer and lower-aquifer systems that form a convergent flow system within a basin bounded by mountains and hills on three sides and discharge to pumping wells and the southern San Francisco Bay. Faults also control the flow of groundwater within the Santa Clara Valley and subdivide the aquifer system into three subregions.After decades of development and groundwater depletion that resulted in substantial land subsidence, Santa Clara Valley Water District (SCVWD) and the local water purveyors have refilled the basin through conservation and importation of water for direct use and artificial recharge. The natural flow system has been altered by extensive development with flow paths toward major well fields. Climate has not only affected the cycles of sedimentation during the glacial periods over the past million years, but interannual to interdecadal climate cycles also have affected the supply and demand components of the natural and anthropogenic inflows and outflows of water in the valley. Streamflow has been affected by development of the aquifer system and regulated flow from reservoirs, as well as conjunctive use of groundwater and surface water. Interaquifer flow through water-supply wells screened across multiple aquifers is an important component to the flow of groundwater and recapture of artificial recharge in the Santa Clara Valley. Wellbore flow and depth-dependent chemical and isotopic data indicate that flow into wells from multiple aquifers, as well as capture of artificial recharge by pumping of water-supply wells, predominantly is occurring in the upper 500 ft (152 m) of the aquifer system. Artificial recharge represents about one-half of the inflow of water into the valley for the period 1970–1999. Most subsidence is occurring below 250 ft

  18. Tar Creek study, Sargent oil field, Santa Clara County, California

    Science.gov (United States)

    Wagner, David L.; Fedasko, Bill; Carnahan, J.R.; Brunetti, Ross; Magoon, Leslie B.; Lillis, Paul G.; Lorenson, T.D.; Stanley, Richard G.

    2002-01-01

    Field work in the Tar Creek area of Sargent oil field was performed June 26 to 28, 2000. The Santa Clara County study area is located in Sections, 30, 31, and 32, Township 11 South, Range 4 East, M.D.B&M; and in Sections 25 and 36, Township 11 South, Range 3 East, M.D.B.&M., north and south of Tar Creek, west of Highway 101. The work was a cooperative effort of the California Department of Conservation's Division of Oil, Gas, and Geothermal Resources (DOGGR), California Geological Survey (CGS), and the United States Geological Survey (USGS). The purpose of the project was to map the stratigraphy and geologic structure (David Wagner, CGS); sample oil for age dating (Les Magoon, USGS); and search for undocumented wells plus conduct a GPS survey of the area (Bill Fedasko, J.P. Carnahan, and Ross Brunetti, DOGGR)

  19. CLARA: A Contemporary Approach to Physics Data Processing

    Science.gov (United States)

    Gyurjyan, V.; Abbott, D.; Carbonneau, J.; Gilfoyle, G.; Heddle, D.; Heyes, G.; Paul, S.; Timmer, C.; Weygand, D.; Wolin, E.

    2011-12-01

    CLARA (CLAS12 Reconstruction and Analysis framework) is CLAS12 physics data processing (PDP) application development framework based on a service oriented architecture (SOA). This framework allows users to design and deploy data processing services as well as dynamically compose PDP applications using available services. Services can be written in Java, C++, and Python languages. The PDP service bus provides a layer on top of a distributed pub-sub middleware implementation. This allows complex service composition and integration without writing a code. We believe that by deviating from the traditional self contained, monolithic PDP application models we can improve maintenance, scalability and quality of physics data analysis. The SOA approach also helps us to separate a specific service programmer from a PDP application designer. Examples of service creation and deployment, along with the CLAS12 track reconstruction application design are presented.

  20. The CLARA/NORSAT-1 solar absolute radiometer: instrument design, characterization and calibration

    Science.gov (United States)

    Walter, Benjamin; Levesque, Pierre-Luc; Kopp, Greg; Andersen, Bo; Beck, Ivo; Finsterle, Wolfgang; Gyo, Manfred; Heuerman, Karl; Koller, Silvio; Mingard, Nathan; Remesal Oliva, Alberto; Pfiffner, Daniel; Soder, Ricco; Spescha, Marcel; Suter, Markus; Schmutz, Werner

    2017-10-01

    The compact lightweight absolute radiometer (CLARA) experiment aims at measuring the total solar irradiance (TSI) in space and is scheduled to fly on the Norwegian NORSAT-1 micro satellite. The CLARA experiment will contribute to the long term monitoring of the TSI variability to support the analysis of potential long term trends in the Sun’s variability. CLARA is traceable to the National Institute of Standards and Technology radiometric scale and will provide further evidence for the TSI value on an absolute scale. In this paper we present the design, characterization, and calibration details of the CLARA instrument. The combined measurement uncertainty for the calibrated SI-traceable CLARA flight instrument is 567-912 ppm (k  =  1) depending on the measuring channel.

  1. COMPLEXITY OF ECOLOGICAL SYSTEMS (THE CASE OF MARINE CILIATE COMMUNITY

    Directory of Open Access Journals (Sweden)

    I.V. Burkovsky

    2016-03-01

    Full Text Available The paper analyzes the seasonal and long-term dynamics of marine interstitial ciliates communities as a result of the processes of system self-organization (of increasing complexity in constantly fluctuating environment. The traits of simple structure of ciliate community include substantial variability in the composition (even in case of stable environment and the lack of sustainable combinations of species. The mechanism of the current state maintenance is the lack of energy supply in certain periods or in specific loci of space, as well as large amplitude and unpredictable fluctuations of environmental factors. An indication of the community’s complexity is availability of stable combinations of species in time and space. The mechanisms of formation of stable species combinations are a constant flow of external energy, optimal values and stability of environmental factors (including repeatability of seasonal cycles and the use of space resources by species according the principle of complementarity of ecological niches.

  2. An approach to measure ciliate grazing on living heterotrophic nanoflagellates

    DEFF Research Database (Denmark)

    Christoffersen, K.; González, J. M.

    2003-01-01

    The complicated routes by which organic material is channelled up to higher trophic levels via bacteria and protozoans is a major issue in aquatic microbial ecology. Because of the fragile nature of protists it is not straightforward to perform experimental studies of prey–predator interactions. ...... a direct approach to measure ciliate grazing specifically on living heterotrophic nanoflagellates.......The complicated routes by which organic material is channelled up to higher trophic levels via bacteria and protozoans is a major issue in aquatic microbial ecology. Because of the fragile nature of protists it is not straightforward to perform experimental studies of prey–predator interactions....... Here we present an approach for the assessment of ciliate grazing on living heterotrophic nanoflagellates. Stationary phase cultures of a heterotrophic nanoflagellate (Cafeteria sp.) were live-stained by allowing them to take up fluorescently labelled macromolecules. Controls revealed that this label...

  3. Social biases determine spatiotemporal sparseness of ciliate mating heuristics

    OpenAIRE

    Clark, Kevin B.

    2012-01-01

    Ciliates become highly social, even displaying animal-like qualities, in the joint presence of aroused conspecifics and nonself mating pheromones. Pheromone detection putatively helps trigger instinctual and learned courtship and dominance displays from which social judgments are made about the availability, compatibility, and fitness representativeness or likelihood of prospective mates and rivals. In earlier studies, I demonstrated the heterotrich Spirostomum ambiguum improves mating compet...

  4. A checklist of ciliate parasites (Ciliophora) of fishes from Mexico.

    Science.gov (United States)

    Aguilar-Aguilar, Rogelio; Islas-Ortega, Alma Gabriela

    2015-10-02

    A database with all available published accounts of the ciliate parasite species of Mexican fishes was assembled. This information, along with records derived from own recent research, allow generating a checklist containing all the records, which is a necessary first step to address future questions in the areas of ecology, evolutionary biology and biogeography of these host-parasite associations. The checklist is presented as a parasite-host list, and a host-parasite list. The checklist contains 30 nominal species, from 9 genera and 8 families of ciliate parasites. Most of the primary records were done for exotic fish species, artificially introduced to Mexico for aquaculture purposes; however, recent works have been conducted in diverse species of native fishes. Excepting one, all the ciliate species listed here have been previously recorded for diverse fish species from different localities around the world. Based on the amount of information contained in this checklist, much more effort is necessary to accurately know the diversity of species of this type of parasites in fish fauna of Mexico.

  5. Methods for taxonomic studies of rumen ciliates (alveolata: ciliophora): a brief review.

    Science.gov (United States)

    Cedrola, Franciane; Rossi, Mariana; Dias, Roberto Júnio Pedroso; Martinele, Isabel; D'Agosto, Marta

    2015-01-01

    This review presents the principal methods used in taxonomic studies of rumen ciliates: live observation, Lugol staining, fixation and staining with methyl-green formalin saline (MFS) solution, protargol staining, silver carbonate impregnation, scanning electron microscopy and molecular techniques. Mastering these techniques is essential for successful research on the taxonomy of rumen ciliates. No single technique reveals all of the characteristics required for a complete description of a rumen ciliate; therefore, it is necessary to combine the use of these techniques as appropriate to the rumen ciliate group under study. Tables are provided to summarize: 1) morphological methods more appropriate for revealing morphological structures of interest, 2) morphological methods indicated for each group of rumen ciliates, and 3) main primers used for PCR amplification of the 18S rDNA of rumen ciliates.

  6. Control of biofilm-dwelling ciliate communities by temperature and resources

    OpenAIRE

    Norf, Helge

    2008-01-01

    Laboratory and field-related experimental approaches were combined to investigate the impacts of temperature and resource enhancements on the development of biofilm-dwelling ciliate communities. The first part of this study concentrated on ciliate community responses towards experimental warming. It was shown that temperature increase during winter can significantly accelerate the early colonization of biofilms by ciliates and enhance the organism density when the resource supply is sufficien...

  7. Rare Freshwater Ciliate Paramecium chlorelligerum Kahl, 1935 and Its Macronuclear Symbiotic Bacterium "Candidatus Holospora parva"

    National Research Council Canada - National Science Library

    Olivia Lanzoni; Sergei I Fokin; Natalia Lebedeva; Alexandra Migunova; Giulio Petroni; Alexey Potekhin

    2016-01-01

    .... In this study, we describe the complex symbiotic system between a very rare ciliate, Paramecium chlorelligerum, unicellular algae inhabiting its cytoplasm, and novel bacteria colonizing the host macronucleus...

  8. Act together-implications of symbioses in aquatic ciliates.

    Science.gov (United States)

    Dziallas, Claudia; Allgaier, Martin; Monaghan, Michael T; Grossart, Hans-Peter

    2012-01-01

    Mutual interactions in the form of symbioses can increase the fitness of organisms and provide them with the capacity to occupy new ecological niches. The formation of obligate symbioses allows for rapid evolution of new life forms including multitrophic consortia. Microbes are important components of many known endosymbioses and their short generation times and strong potential for genetic exchange may be important drivers of speciation. Hosts provide endo- and ectosymbionts with stable, nutrient-rich environments, and protection from grazers. This is of particular importance in aquatic ecosystems, which are often highly variable, harsh, and nutrient-deficient habitats. It is therefore not surprising that symbioses are widespread in both marine and freshwater environments. Symbioses in aquatic ciliates are good model systems for exploring symbiont-host interactions. Many ciliate species are globally distributed and have been intensively studied in the context of plastid evolution. Their relatively large cell size offers an ideal habitat for numerous microorganisms with different functional traits including commensalism and parasitism. Phagocytosis facilitates the formation of symbiotic relationships, particularly since some ingested microorganisms can escape the digestion. For example, photoautotrophic algae and methanogens represent endosymbionts that greatly extend the biogeochemical functions of their hosts. Consequently, symbiotic relationships between protists and prokaryotes are widespread and often result in new ecological functions of the symbiotic communities. This enables ciliates to thrive under a wide range of environmental conditions including ultraoligotrophic or anoxic habitats. We summarize the current understanding of this exciting research topic to identify the many areas in which knowledge is lacking and to stimulate future research by providing an overview on new methodologies and by formulating a number of emerging questions in this field.

  9. Social biases determine spatiotemporal sparseness of ciliate mating heuristics.

    Science.gov (United States)

    Clark, Kevin B

    2012-01-01

    Ciliates become highly social, even displaying animal-like qualities, in the joint presence of aroused conspecifics and nonself mating pheromones. Pheromone detection putatively helps trigger instinctual and learned courtship and dominance displays from which social judgments are made about the availability, compatibility, and fitness representativeness or likelihood of prospective mates and rivals. In earlier studies, I demonstrated the heterotrich Spirostomum ambiguum improves mating competence by effecting preconjugal strategies and inferences in mock social trials via behavioral heuristics built from Hebbian-like associative learning. Heuristics embody serial patterns of socially relevant action that evolve into ordered, topologically invariant computational networks supporting intra- and intermate selection. S. ambiguum employs heuristics to acquire, store, plan, compare, modify, select, and execute sets of mating propaganda. One major adaptive constraint over formation and use of heuristics involves a ciliate's initial subjective bias, responsiveness, or preparedness, as defined by Stevens' Law of subjective stimulus intensity, for perceiving the meaningfulness of mechanical pressures accompanying cell-cell contacts and additional perimating events. This bias controls durations and valences of nonassociative learning, search rates for appropriate mating strategies, potential net reproductive payoffs, levels of social honesty and deception, successful error diagnosis and correction of mating signals, use of insight or analysis to solve mating dilemmas, bioenergetics expenditures, and governance of mating decisions by classical or quantum statistical mechanics. I now report this same social bias also differentially affects the spatiotemporal sparseness, as measured with metric entropy, of ciliate heuristics. Sparseness plays an important role in neural systems through optimizing the specificity, efficiency, and capacity of memory representations. The present

  10. Act together – implications of symbioses in aquatic ciliates

    Directory of Open Access Journals (Sweden)

    Claudia eDziallas

    2012-08-01

    Full Text Available Mutual interactions in form of symbioses can increase the fitness of organisms and provide them with the capacity to occupy new ecological niches. The formation of obligate symbioses allows for rapid evolution of new life forms including multitrophic consortia. Microbes are important components of many known endosymbioses and their short generation times and strong potential for genetic exchange may be important drivers of speciation. Hosts provide endo- and ectosymbionts with stable, nutrient-rich environments and protection from environmental stresses. This is of particular importance in aquatic ecosystems, which are often highly variable, harsh and nutrient-deficient habitats. Thus it is not surprising that symbioses are widespread in both marine and freshwater environments. Symbioses in aquatic ciliates are good model systems for exploring symbiont-host interactions. Many ciliate species are globally distributed and have been intensively studied in the context of plastid evolution. Their relatively large cell size offers an ideal habitat for numerous microorganisms with different functional traits including commensalism and parasitism. Phagocytosis facilitates the formation of symbiotic relationships, particularly since some ingested microorganisms can escape the digestion. For example, photoautotrophic algae and methanogens represent endosymbionts that greatly extend the biogeochemical functions of their hosts. Consequently, symbiotic relationships between protists and prokaryotes are widespread and often result in new ecological functions of symbiotic communities. This enables ciliates to thrive under a wide range of environmental conditions including ultraoligotrophic or anoxic habitats. We summarize the current understanding of this exciting research topic to identify the many areas in which knowledge is lacking and to stimulate future research by providing an overview on new methodologies and by formulating a number of emerging

  11. A new marine ciliate, Amphileptus litonotiformis nov. SP. (Protozoa Ciliophora)

    Science.gov (United States)

    Song, Weibo

    1991-12-01

    The morphology and infraciliature of a marine ciliate Anphileptus litonotiformis nov. sp. inhabiting a shrimp farming pond in Sheyang (Jiangsu Province) are described. According to the infraciliature, habitat, and other morphological characters, it can not be congeneric with any other species of the genus. The special features of A. litonotiformis are the Litonotus-like body shape and the arrangement of its extrusomes regularly located along the margin of the cytostome. The most important characters of some related Anphileptus-species from marine water are biometrically tabled and discussed.

  12. The Compact Lightweight Absolute Radiometer (CLARA) for Total Solar Irradiance Measurements on the NORSAT-1 Satellite

    Science.gov (United States)

    Walter, Benjamin; Finsterle, Wolfgang; Koller, Silvio; Levesque, Pierre-Luc; Pfiffner, Daniel; Schmutz, Werner

    2017-04-01

    Continuous and precise Total Solar Irradiance (TSI) measurements are indispensable to evaluate the influence of short- and long-term solar variability on the Earth's energy budget. The existence of a potential long-term trend in the suns activity and whether or not such a trend could be climate effective is still a matter of debate. The Compact Lightweight Absolute Radiometer (CLARA) is one of PMOD/WRC's future contributions to the almost seamless series of space borne TSI measurements since 1978. CLARA was designed and built by PMOD/WRC and characterized and calibrated by PMOD/WRC as part of the "European Metrology Research Program" (EMRP) project "European Metrology for Earth Observation and Climate" (MetEOC-2) funded by the European Commission. The main goals of the CLARA experiment are to continue the TSI data record with high accuracy and precision and to facilitate monitoring with its compact and adaptable design. CLARA will be one of three payloads of the Norwegian micro satellite NORSAT-1, along with Langmuir probes for space plasma research and an Automatic Identification System (AIS) receiver to monitor maritime traffic in Norwegian waters. The launch of NORSAT-1 is planned for March 2017. We present the design and calibration of CLARA, a new generation of Electrical Substitution Radiometers (ESR) comprising the latest radiometer developments of PMOD/WRC: i) A three-detector design for degradation tracking and redundancy, ii) a digital control system, iii) a new reference block and detector design to minimize size and weight of the instrument. The characterization of the CLARA instrument components provides an overview on the improvements that were achieved with the latest radiometer developments. An end-to-end calibration of CLARA against the SI-traceable cryogenic radiometer of the TSI Radiometer Facility (TRF) at the Laboratory for Atmospheric and Space Physics (LASP) in Boulder (Colorado) results in a combined measurement uncertainty of 0.05% (k = 1

  13. Foam cell formation of alveolar macrophages in Clara cell ablated mice inhaling crystalline silica.

    Science.gov (United States)

    Yatera, Kazuhiro; Morimoto, Yasuo; Kim, Heung-Nam; Myojo, Toshihiko; Mukae, Hiroshi

    2011-10-01

    We investigated the function of Clara cells in vivo during exposure to inhaled crystalline silica by morphological and immunohistochemical examination of intra-alveolar cells and alveolar macrophages in Clara cell-ablated mice. The Clara cells of male FVB/n mice (8-12 weeks old) were ablated by intraperitoneal administration of naphthalene (300 mg/kg). The mice were then exposed to crystalline silica (Min-U-Sil-5, 97.1 ± 9.5 mg/m³, 6 hours/day, 5 days/week) for up to two weeks. The lungs were assessed by morphometry, as well as by immunohistochemistry of CD36, lectin-like oxygenated low-density lipoprotein receptor (LOX)-1, and matrix metalloproteinases (MMPs) -2, -9 and -12. There was a significantly greater number of intra-alveolar cells in Clara cell-ablated mouse groups than in wild-type mouse groups that were exposed to crystalline silica. A marked number of foamy alveolar macrophages were only detected in the Clara cell-ablated group exposed to crystalline silica, indicating that Clara cells inhibit infiltration and foam cell formation of alveolar macrophages. Immunohistochemical analysis indicated that foamy alveolar macrophages in the Clara cell-ablated group that inhaled crystalline silica overexpress CD36 and LOX-1, indicating upregulation of scavenger receptors of alveolar macrophages. These cells also express MMP-2, -9 and -12, suggesting increased gelatinolytic and elastolytic activities. Our findings suggest that Clara cells not only inhibit infiltration of alveolar macrophages but also their phagocytotic and gelatinolytic functions in silica-induced pulmonary injury.

  14. Ciliate communities of a large shallow lake: association with macrophyte beds.

    Science.gov (United States)

    Karus, Katrit; Feldmann, Tõnu; Nõges, Peeter; Zingel, Priit

    2014-08-01

    We investigated the influence of macrophyte composition on ciliate community structure in a large, shallow, eutrophic Lake Võrtsjärv. We hypothesized that macrophyte composition must have strong influence on the dispersal of ecologically different ciliate groups in a shallow lake and that more diverse macrophyte stands cause also a greater diversity in the ciliate community. In Võrtsjärv macrophyte distribution is spatially strongly polarized both in east-west and north-south directions in relation to abiotic factors. Phragmites australis and Myriophyllum spicatum were the most widespread species occurring in most parts of the lake. Correlation of environmental, macrophyte and planktonic ciliate variables confirmed the suggested spatial gradients. More diverse macrophyte stands supported a high species richness and abundance of epiplanktonic community but showed negative influence on the number and abundance of euplanktonic ciliate taxa. Opposite trends were found relative to the abundance of P. australis. Benthic ciliates showed a similar distribution pattern to euplanktonic taxa being most abundant in sites were the Shannon-Weaver index for macrophytes was low. Strong polarizing effect of the lake's vegetation on planktonic ciliate diversity was reflected in correlations of the number of ciliate taxa as well as the numbers of eu- and epiplanktonic taxa with geographic co-ordinates. Copyright © 2014 Elsevier GmbH. All rights reserved.

  15. Planktonic ciliate community structure in shallow lakes of lowland Western Europe.

    Science.gov (United States)

    Van Wichelen, Jeroen; Johansson, Liselotte S; Vanormelingen, Pieter; Declerck, Steven A J; Lauridsen, Torben L; De Meester, Luc; Jeppesen, Erik; Vyverman, Wim

    2013-11-01

    Temperate shallow meso- to eutrophic lakes can exist in one of two alternative states with contrasting foodwebs, referred to as the clear-water and the turbid state. We describe the planktonic ciliate communities of such lakes based on a survey of 66 northwestern European lakes. Ciliates were enumerated and identified to species level according to the quantitative protargol staining technique. Ciliate biomass was on average twice as high in the turbid than in the clear-water lakes. The ciliate communities were dominated by oligotrichs and protostomatids, and no differences in functional composition or α-diversity could be detected between turbid and clear-water lakes, although β-diversity tended to be higher in the latter. At the species level, however, community structure strongly differed between turbid and clear-water lakes, and several indicator species could be identified for the different lake categories. Variation partitioning showed that nutrient status did not explain ciliate community structure independent of the alternative states, while lake area was identified as an additional structuring factor for the ciliate communities. These results stress the importance of the ecosystem structure in shaping ciliate communities in temperate shallow lakes and suggest that nutrient status has little direct effect on ciliate community structure in such lakes. Copyright © 2013 Elsevier GmbH. All rights reserved.

  16. Two Functionally Distinct Ciliates Dwelling in Acropora Corals in the South China Sea near Sanya, Hainan Province, China▿

    Science.gov (United States)

    Qiu, Dajun; Huang, Liangmin; Huang, Hui; Yang, Jianhui; Lin, Senjie

    2010-01-01

    We detected and characterized two distinct scuticociliate ciliates inside Acropora corals in the South China Sea. One, voraciously foraging on Symbiodinium, resembled the brown band disease of ciliates. The other, which is closely related to Paranophrys magna, grazed on detritus instead of Symbiodinium. These two ciliates may serve contrasting functions (competitor versus “cleaner”) in the coral-ciliate-Symbiodinium triangular relationship. PMID:20581178

  17. Intestinal ciliate composition found in the feces of racing horses from Izmir, Turkey.

    Science.gov (United States)

    Gürelli, Gözde; Göçmen, Bayram

    2012-08-01

    Species composition and distribution of intestinal ciliates were investigated in the feces from 15 racing horses living near Izmir, Turkey. Thirty-seven species belonging to 21 genera were identified. Although no new species were observed, this is the first report on intestinal ciliates in racing horses living in Turkey. The mean number of ciliates was 26.4 ± 13.9 × 10(4) cells ml(-1) of feces and the mean number of ciliate species per host was 18.8 ± 7.1. No ciliates were observed in one horse. Bundleia and Polymorphella were found to be the two dominant genera, occurring in high proportions. In contrast, Didesmis and Prorodonopsis were only observed at a low frequency. Bundleia nana, Blepharoconus hemiciliatus, Paraisotrichopsis composita, Prorodonopsis coli and Spirodinium equi were newly recorded from Turkey. Copyright © 2012 Elsevier GmbH. All rights reserved.

  18. REGISTRO HOSPITALARIO DE INFARTO AGUDO DE MIOCARDIO EN SANTA CLARA / Hospital registry of acute miocardial infarction in Santa Clara

    Directory of Open Access Journals (Sweden)

    Alberto Morales Salinas

    2010-09-01

    Full Text Available Resumen Introducción y objetivos: En Cuba las estadísticas vitales de la morbi-mortalidad por infarto agudo de miocardio proceden de pequeñas áreas de salud. El objetivo fue identificar las principales características y conducta hospitalaria de los pacientes con infarto a través de un registro hospitalario de infarto. Métodos: Se monitorizaron los casos de infarto ocurridos en pacientes de 45 a 74 años de edad y residentes en Santa Clara, durante los años 2007 y 2008. Las fuentes de información fueron: listados de egresos hospitalarios, registros hospitalarios de infarto de miocardio, certificados de defunción y registros de autopsias nosocomiales. Se utilizó la metodología MONICA (MONItoring Trends and Determinants in CArdiovascular Disease Project. Resultados: Se registraron 297 pacientes con infarto. El 62,7 % de los varones y el 46,8 % de las mujeres recibieron tratamiento de reperfusión. Las letalidades hospitalarias en varones y mujeres fueron de 25,3 % y 45 %. Las variables que se relacionaron con una mayor mortalidad fueron la edad, el sexo femenino, la frecuencia cardíaca alta, el infarto de topografía anterior, la creatinina elevada, así como los antecedentes personales de cardiopatía isquémica previa y diabetes mellitus. Mientras que el tratamiento con fibrinolíticos y/o betabloqueadores se correlacionaron inversamente con la mortalidad. Conclusiones: Existió una elevada prevalencia de dislipidemia desconocida y letalidad hospitalaria a 28 días; así como una baja utilización de las estrategias cruentas. La hipertensión sistólica al ingreso se relacionó con un mejor pronóstico. / Abstract Introduction and objectives: In Cuba, the vital statistics of morbi-mortality due to acute myocardial infarction come from small health areas. The objective of this study was to identify the main characteristics and hospital management of patients with myocardial infarction, through a hospital registry of infarctions

  19. Report of a case of surgical ciliated cyst in the left maxillary sinus

    Directory of Open Access Journals (Sweden)

    Amirhossein Pakravan

    2017-06-01

    Full Text Available Surgical ciliated cyst happens as a delayed complication in the maxillary sinus, and is more frequent in Asia in comparison with Western countries. We report a case of surgical ciliated cyst in maxillary sinus in a male patient after 30 years of surgery for sinusitis treatment. The patient had swelling and pain in the region, and his radiographic views showed a cystic lesion. Incisional biopsy was performed and surgical ciliated cyst was reported as diagnosis. Then, Enucleation with curettage was performed for him as treatment.

  20. Entrapment of ciliates at the water-air interface.

    Directory of Open Access Journals (Sweden)

    Jonathan Ferracci

    Full Text Available The importance of water-air interfaces (WAI on microorganism activities has been recognized by many researchers. In this paper, we report a novel phenomenon: the entrapment of ciliates Tetrahymena at the WAI. We first characterized the behavior of cells at the interface and showed that the cells' swimming velocity was considerably reduced at the WAI. To verify the possible causes of the entrapment, we investigated the effects of positive chemotaxis for oxygen, negative geotaxis and surface properties. Even though the taxes were still effective, the entrapment phenomenon was not dependent on the physiological conditions, but was instead affected by the physical properties at the interface. This knowledge is useful for a better understanding of the physiology of microorganisms at interfaces in nature and in industry.

  1. RNA-mediated transgenerational inheritance in ciliates and plants.

    Science.gov (United States)

    Neeb, Zachary T; Nowacki, Mariusz

    2017-12-11

    In the age of next-generation sequencing (NGS) and with the availability of whole sequenced genomes and epigenomes, some attention has shifted from purely sequence-based studies to those of heritable epigenetic modifications. Transgenerational inheritance can be defined as heritable changes to the state of DNA that may be passed on to subsequent generations without alterations to the underlying DNA sequence. Although this phenomenon has been extensively studied in many systems, studies of transgenerational inheritance in mammals and other higher-level eukaryotes may be complicated by the fact that many epigenetic marks are reprogrammed during sexual reproduction. This, by definition, may obscure our interpretation of what is in fact truly transgenerational. Therefore, in this mini review, we discuss what is currently known in the field about transgenerational epigenetic inheritance in ciliates and plants, with a particular emphasis on RNA-mediated processes and changes in chromatin states.

  2. A ciliate memorizes the geometry of a swimming arena

    Science.gov (United States)

    Kunita, Itsuki; Yamaguchi, Tatsuya; Tero, Atsushi; Akiyama, Masakazu; Kuroda, Shigeru; Nakagaki, Toshiyuki

    2016-01-01

    Previous studies on adaptive behaviour in single-celled organisms have given hints to the origin of their memorizing capacity. Here we report evidence that a protozoan ciliate Tetrahymena has the capacity to learn the shape and size of its swimming space. Cells confined in a small water droplet for a short period were found to recapitulate circular swimming trajectories upon release. The diameter of the circular trajectories and their duration reflected the size of the droplet and the period of confinement. We suggest a possible mechanism for this adaptive behaviour based on a Ca2+ channel. In our model, repeated collisions with the walls of a confining droplet result in a slow rise in intracellular calcium that leads to a long-term increase in the reversal frequency of the ciliary beat. PMID:27226383

  3. Plan estratégico para la galería multicentro Santa Clara

    OpenAIRE

    Lay Chung, Dennis; Lazaro Ruíz, Carlos; Randich Tejeda, Christian

    2015-01-01

    El presente trabajo se refiere al Planeamiento Estratégico de la empresa Galerías Multicentro Santa Clara, el cual nace de un emprendimiento familiar, a inicios del año 2005, con la finalidad de satisfacer la demanda de locales comerciales en la zona de Santa Clara. El negocio de la empresa es el alquiler de espacios para desarrollar actividades comerciales, dentro de ese contexto, el desarrollo y liderazgo que ha tenido la empresa le ha permitido establecerse como un importante centro adonde...

  4. Reglas electorales claras: el objetivo de todo organismo encargado de organizar un torneo comicial

    Directory of Open Access Journals (Sweden)

    Alejandro J. Robles Leal

    2017-07-01

    Full Text Available La importancia de establecer reglas claras y que estas sean conocidas por todos los actores electorales coadyuva al desarrollo exitoso de una contienda electoral. El artículo analiza las sentencias 1532-E1-2017 y 2173-E1-2017 del Tribunal Supremo de Elecciones las cuales, en términos generales, han dejado clara la obligación que tienen los partidos políticos de definir las reglas que regirán las contiendas electorales dentro de las agrupaciones políticas.

  5. Clara Barton: teacher, nurse, Civil War heroine, founder of the American Red Cross.

    Science.gov (United States)

    Evans, Gerald D

    2003-01-01

    Clara Barton was a nineteenth century woman driven to greatness. She was a teacher, a nurse, a Civil War heroine and founder of the American Red Cross. In order to cut a path into the future we must know where we have been. The story of Clara Barton is about someone who cut that path. It is about courage, overcoming obstacles, never giving up and doing the job that needs doing. What makes it fascinating is the human side, the weaknesses that coloured her life. We can learn from her journey as we develop our own path into the future.

  6. New records of epibiont Ciliates (Ciliophora) from Indian coast with descriptions of six new species

    Digital Repository Service at National Institute of Oceanography (India)

    Chatterjee, T.; Fernandez-Leborans, G.; Ramteke, D.; Ingole, B.S.

    Six epibiont ciliates Cothurnia palkensis, C. nova, C. disper, C. dovgali, C. mannaerensis and C. clampi are described as new for science from the Indian coast. Acineta truncata Collin, 1909 and A. tuberosa Ehrenberg, 1834 are reported here...

  7. Composition and distribution of planktonic ciliates from ponds of different salinity in the solar saltwork of Sfax, Tunisia

    Science.gov (United States)

    Elloumi, Jannet; Carrias, Jean-François; Ayadi, Habib; Sime-Ngando, Télesphore; Boukhris, Mekki; Bouaïn, Abderrahmen

    2006-03-01

    The planktonic ciliated protozoa of 14 ponds of increasing salinity were investigated in the saline of Sfax, Tunisia. Taxa of the classes of Spirotrichea and Heterotrichea were the numerous ciliates. Abundance of the community ranged from 0.0 to 11.8 × 10 4 ciliates per litre. Values decrease significantly with salinity gradient, as species richness does. Based on the range of salinity over which ciliate taxa appeared, we distinguished three groups of ciliates. The first group is mainly composed of oligotrichs and choreotrichs that are commonly found in marine coastal waters. Small ciliates belonging to the order Prostomatida were found in a large range of salinity values, but their densities also decreased with salt concentration. In contrast, large-size species of heterotrichous ciliates were found in ponds with high salinity values only. In these ponds, the presence of prey appeared as an important factor in controlling the abundances of these halotolerant ciliates. Our data also suggest that Fabrea salina, a common halophile ciliate, acts as a competitor of the brine shrimp Artemia salina in the saline of Sfax. Salinity, prey availability, and the presence of competitors seem to be the main factors for the distribution of ciliate taxa in this hypersaline environment.

  8. Immunoexpression of P16INK4a, Rb and TP53 proteins in bronchiolar columnar cell dysplasia (BCCD in lungs resected due to primary non-small cell lung cancer.

    Directory of Open Access Journals (Sweden)

    Lech Chyczewski

    2008-02-01

    Full Text Available Lung cancer is the leading cause of death worldwide. High mortality comes out mainly of the fact that majority of the cases are diagnosed in advanced stadium. An expanded diagnostics of precancerous conditions would certainly contribute to lowering the mortality rate. Many of the molecular changes accompanying the multistep cancer development could be observed using the immunohistochemistry method. In this paper we describe the morphology and cell cycle proteins immunoexpression of the novel probable preinvasive lesion - bronchiolar columnar cell dysplasia (BCCD. Thirty cases of BCCD selected out of 193 patients population, treated for primary non-small cell lung cancer were investigated. Loss of P16INK4a protein was observed in 70% of all cases and was statistically significant in patients with adenocarcinoma. Two cases show abnormal cytoplasmic localization of this protein. TP53 protein accumulates in 26.7% of all BCCD. Rb protein was active in 48.3% of the BCCD cases. In two cases we observed differentiation of the cells composing BCCD into multilayer epithelium of the squamous type, which occurs with formation of desmosomes. We suppose that BCCD may be preneoplastic lesion leading to adenocarcinoma as well as to peripheral squamous cell lung cancer.

  9. Ciliate epibionts associated with crustacean zooplankton in German lakes: Distribution, motility and bacterivory

    Directory of Open Access Journals (Sweden)

    Samantha Lynn Bickel

    2012-07-01

    Full Text Available Ciliate epibionts associated with crustacean zooplankton are widespread in aquatic systems, but their ecological roles are little known. We studied the occurrence of ciliate epibionts on crustacean zooplankton in nine German lakes with different limnological features during the summer of 2011. We also measured the detachment and reattachment rates of the ciliates, changes in their motility, and the feeding rates of attached vs. detached ciliate epibionts. Epibionts were found in all lakes sampled except an acidic lake with large humic inputs. Epibiont prevalence was as high as 80.96% on the cladoceran Daphnia cucullata, 67.17% on the cladoceran Diaphanosoma brachyurum, and 46.67% on the calanoid copepod Eudiaptomus gracilis. Both cladoceran groups typically had less than 10 epibionts per individual, while the epibiont load on E. gracilis ranged from one to >30 epibionts per individual. After the death of the zooplankton host, the peritrich ciliate epibiont Epistylis sp. detached in an exponential fashion with a half-life of 5 min, and 98% detached within 30 min, leaving behind the stalks used for attachment. Immediately after detachment, the ciliates were immotile, but 62% became motile within 60 min. When a new host was present, only 27% reattached after 120 min. The average measured ingestion rate and clearance rate of Epistylis were 11,745 bacteria ciliate-1 h-1 and 24.33 μl ciliate-1 h-1, respectively. Despite their high feeding rates, relatively low epibiont abundances were observed in the field, which suggests either diversion of energy to stalk formation, high metabolic loss by the epibionts, or high mortality among the epibiont populations.

  10. Freshwater biodiversity of Guam. 1. Introduction, with new records of ciliates and a heliozoan

    OpenAIRE

    LOBBAN, CHRISTOPHER S.; SCHEFTER, MARÍA

    2008-01-01

    Inland waters are the most endangered ecosystems in the world because of complex threats and management problems, yet the freshwater microbial eukaryotes and microinvertebrates are generally not well known and from Guam are virtually unknown. Photodocumentation can provide useful information on such organisms. In this paper we document protists from mostly lentic inland waters of Guam and report twelve freshwater ciliates, especially peritrichs, which are the first records of ciliates from Gu...

  11. Ciliates rapidly enhance the frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles.

    Science.gov (United States)

    Matsuo, Junji; Oguri, Satoshi; Nakamura, Shinji; Hanawa, Tomoko; Fukumoto, Tatsuya; Hayashi, Yasuhiro; Kawaguchi, Kouhei; Mizutani, Yoshihiko; Yao, Takashi; Akizawa, Kouzi; Suzuki, Haruki; Simizu, Chikara; Matsuno, Kazuhiko; Kamiya, Shigeru; Yamaguchi, Hiroyuki

    2010-10-01

    The mechanism underlying bacterial conjugation through protozoa was investigated. Kanamycin-resistant Escherichia coli SM10λ+ carrying pRT733 with TnphoA was used as donor bacteria and introduced by conjugation into ciprofloxacin-resistant E. coli clinical isolate recipient bacteria. Equal amounts of donor and recipient bacteria were mixed together in the presence or absence of protozoa (ciliates, free-living amoebae, myxamoebae) in Page's amoeba saline for 24 h. Transconjugants were selected with Luria broth agar containing kanamycin and ciprofloxacin. The frequency of conjugation was estimated as the number of transconjugants for each recipient. Conjugation frequency in the presence of ciliates was estimated to be approximately 10⁻⁶, but in the absence of ciliates, or in the presence of other protozoa, it was approximately 10⁻⁸. Conjugation also occurred in culture of ciliates at least 2 h after incubation. Successful conjugation was confirmed by the polymerase chain reaction. Addition of cycloheximide or latrunculin B resulted in suppression of conjugation. Heat killing the ciliates or bacteria had no effect on conjugation frequency. Co-localization of green fluorescent protein-expressing E. coli and PKH-67-vital-stained E. coli was observed in the same ciliate vesicles, suggesting that both donor and recipient bacteria had accumulated in the same vesicle. In this study, the conjugation frequency of bacteria was found to be significantly higher in vesicles purified from ciliates than those in culture suspension. We conclude that ciliates rapidly enhance the conjugation of E. coli strains through bacterial accumulation in vesicles. Copyright © 2010 Elsevier Masson SAS. All rights reserved.

  12. Species Diversity of Ciliates in Forest Soils of the Samur-Yalama National Park

    Directory of Open Access Journals (Sweden)

    Mamedova V. F.

    2016-04-01

    Full Text Available The results of studies on soil ciliate diversity in different parts of the Samur-Yalama National Park undergoing human impact of different force are presented. It is shown that the highest species diversity observed in forest soils with minimal human impact. Cultivated soils in gardens and farmlands are characterized by low species diversity, often with mass development of a few dominant eurybiont species. A strong influence of land treatment on soil fauna of ciliates shown.

  13. Anaerobic ciliates from a sulphide-rich solution lake in Spain.

    Science.gov (United States)

    Finlay, B J; Clarke, K J; Vicente, E; Miracle, M R

    1991-06-21

    We have examined and quantified the anaerobic ciliates living in the hypolimnion of a 14 m deep sulphide-rich (up to 0.73 mM) solution lake in Spain. At least seven ciliate species were found, numbering up to 50 ml-1 in total and reaching maximum abundance close to the sediment. Caenomorpha medusula, Lacrymaria elegans, L. sapropelica and Lagynus sp. were the most abundant species. Their vertical distributions were not related to the sulphide profile. Most ciliates were dependent on the sedimentation of cryptomonads, photosynthetic bacteria (especially Chromatium and Oscillatoria) and other bacteria from their sites of production in closely-juxtaposed mid-water plates. All anaerobic ciliates contained at least one type of symbiotic bacterium which showed methanogen autofluorescence. C. medusula, Lagynus sp. and Lacrymaria sapropelica also contained a large, non-fluorescing rod-shaped bacterium. In C. medusula, the methanogens and the non-fluorescing rods were both attached to the hydrogenosomes. In this ciliate alone, a third bacterial type was attached to the external ventral surface of the ciliate. Digestion of sulphide-oxidising bacteria by ciliates which harbour methanogenic bacteria provides a short bridge between the anaerobic sulphur and carbon cycles. Theoretical considerations of the rate of ciliate consumption of microbial carbon in the anoxic hypolimnion indicate that it is significant and that it may amount to 4 × 10(-5) g cm(-2)d(-1). Copyright © 1991 Gustav Fischer Verlag · Stuttgart · Jena · New York. Published by Elsevier GmbH.. All rights reserved.

  14. Comparing the responses of rumen ciliate protozoa and bacteria to excess carbohydrate.

    Science.gov (United States)

    Teixeira, César R V; Lana, Rogério de Paula; Tao, Junyi; Hackmann, Timothy J

    2017-06-01

    When given excess carbohydrate, certain microbial species respond by storing energy (synthesizing reserve carbohydrate), but other species respond by dissipating the energy as heat (spilling energy). To determine the importance of these responses in the rumen microbial community, this study quantified the responses of mixed ciliate protozoa vs bacteria to glucose. We hypothesized that ciliates would direct more glucose to synthesis of reserve carbohydrate (and less to energy spilling) than would bacteria. Ciliates and bacteria were isolated from rumen fluid using filtration and centrifugation, resuspended in nitrogen-free buffer to limit growth, and dosed with 5 mM glucose. Compared with bacteria, ciliates consumed glucose >3-fold faster and synthesized reserve carbohydrate 4-fold faster. They incorporated 53% of glucose carbon into reserve carbohydrate-nearly double the value (27%) for bacteria. Energy spilling was not detected for ciliates, as all heat production (104%) was accounted by synthesis of reserve carbohydrate and endogenous metabolism. For bacteria, reserve carbohydrate and endogenous metabolism accounted for only 68% of heat production, and spilling was detected within 11 min of dosing glucose. These results suggest that ciliates alter the course of ruminal carbohydrate metabolism by outcompeting bacteria for excess carbohydrate, maximizing reserve carbohydrate synthesis, and minimizing energy spilling. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  15. Mucous and ciliated cell metaplasia in epithelial linings of odontogenic inflammatory and developmental cysts.

    Science.gov (United States)

    Takeda, Yasunori; Oikawa, Yuko; Furuya, Izuru; Satoh, Masanobu; Yamamoto, Hirotsugu

    2005-06-01

    The incidence of mucous and ciliated cells in epithelial linings was examined among odontogenic inflammatory cysts (radicular cysts) and developmental cysts (dentigerous and primordial cysts). Mucous cells were found in 20.8% of all cysts examined, while ciliated cells were found in 11.4%; however, ciliated cells were always accompanied by mucous cells. The incidence of mucous cells in radicular cysts and dentigerous cysts and that of ciliated cells in radicular cysts was higher in the maxilla than in the mandible, while the incidence of mucous cells in primordial cysts and that of ciliated cells in dentigerous cysts and primordial cysts was higher in the mandible than in the maxilla. The present results regarding mucous cells and ciliated cells in the epithelial linings of intraosseous odontogenic cysts indicate a metaplasic origin, but the cause and biological significance of this phenomenon is not known. Mucous cells were present in the surface layer of epithelial linings, and intraepithelial gland-like structures lined with mucous cells were observed in the hyperplastic regions of epithelial linings of several radicular and dentigerous cysts. Such gland-like structures lined by mucous cells in the thickened epithelial lining, which have not been demonstrated previously, resembled the glandular structures of "glandular odontogenic cysts".

  16. Ephemera danica (Ephemeroptera: Ephemeridae) As a Resource for Two Commensals: Ciliated Protozoans (Sessilida) and Chironomids (Diptera).

    Science.gov (United States)

    Grzybkowska, Maria; Szczerkowska-Majchrzak, Eliza; Dukowska, Małgorzata; Leszczyńska, Joanna; Przybylski, Mirosław

    2016-01-01

    The distribution and coexistence of two unrelated commensals, the chironomid Epoicocladius ephemerae (Kieffer 1924) and ciliate Carchesium polypinum L. 1758, on one host species, Ephemera danica Muller 1764, sampled in two small lowland rivers in 2009, 2010 and 2011, were investigated. We analyzed 288 mayfly specimens from the Bzura River and 101 from the Mroga River. The number of commensals on a single mayfly specimen varied between 0 and 18 chironomids, and from 0 to 46 colonies of ciliates. Prevalences were >48% for chironomids and ∼30% for ciliates, whereas mean intensities were low (4.01±6.04 commensals on one host). The spatial distribution of each commensal species was investigated on different parts of the host body. Neither chironomids nor ciliates infected the whole mayfly body. The co-occurrence of these two commensals was not random and showed a negative association. Chironomids were most frequent on two or three parts of the body (two parts of the abdomen, with gills and without gills, and legs), whereas ciliates were found on two parts (the whole abdomen). Coexistence of the two commensal species led to partitioning of resources that was host body size dependent: small mayflies (optimal size 11.63 mm) were primarily settled by ciliated protozoans while larger specimens (optimal size 28.77 mm) were settled by chironomids. © The Author 2016. Published by Oxford University Press on behalf of the Entomological Society of America.

  17. 75 FR 8106 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2010-02-23

    ..., and San Mateo Counties, CA AGENCY: Fish and Wildlife Service, Interior. ACTION: Notice of intent to... located in Alameda, Santa Clara, and San Mateo Counties of California. We provide this notice in... Clara, and San Mateo Counties, CA. This notice complies with our CCP policy to (1) advise other Federal...

  18. 77 FR 28895 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2012-05-16

    ... Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo Counties, CA AGENCY: Fish and Wildlife Service, Interior. ACTION: Notice of availability... Wildlife Refuge, located in the Alameda, Santa Clara, and San Mateo Counties of California, consists of...

  19. 31 flavors to 50 shades of grey: battling Phytophthoras in native habitats managed by the Santa Clara Valley Water District

    Science.gov (United States)

    Janet Hillman; Tedmund J. Swiecki; Elizabeth A. Bernhardt; Heather K. Mehl; Tyler B. Bourret; David Rizzo

    2017-01-01

    The Santa Clara Valley Water District (District) is a wholesale water supplier for 1.8 million people in Santa Clara County, California. Capital, water utility, and stream maintenance projects result in extensive, long-term mitigation requirements in riparian, wetland, and upland habitats throughout the county. In 2014, several restoration sites on the valley floor and...

  20. 78 FR 67336 - Habitat Conservation Plan for the United Water Conservation District, Santa Clara River Watershed...

    Science.gov (United States)

    2013-11-12

    ..., operations, and maintenance of water management facilities within the lower Santa Clara River watershed... water management activities. United intends to request a 50-year permit covering five species federally... describe direct, indirect, and cumulative impacts on biological resources, land use, air quality, water...

  1. Arachidonic acid metabolism and regulation of ion transport in rabbit Clara cells

    Energy Technology Data Exchange (ETDEWEB)

    Van Scott, M.R.; McIntire, M.R.; Henke, D.C. (Univ. of North Carolina, Chapel Hill (USA))

    1990-10-01

    Sonicates of freshly isolated Clara cells produced thromboxane B2 (TxB2), prostaglandin (PG) D2, PGE2, PGF2 alpha, hydroxyheptadecatrienoic acid (HHT), and 12-hydroxyeicosatetraenoic acid (12-HETE) as detected using high-performance liquid chromatography (HPLC). Sonicates of Clara cells cultured on collagen matrices produced the same metabolites. Rates of (3H)arachidonic acid metabolism increased in culture, but the changes were not associated with changes in cell number. Sonicates of freshly isolated tracheal cells produced mainly 12-HETE. Cyclooxygenase products were not produced consistently. Sonicates of tracheal cultures produced significant quantities of TxB2, PGD2, PGE2, PGF2 alpha, and HHT, but 12-HETE remained the major metabolite. Equivalent short-circuit current (Ieq) across cultured Clara cell epithelia was unaffected by bilateral exposure to TxB2, PGD2, PGE2, PGF2 alpha, HHT, or 12-HETE. A minor (1%) decrease in transepithelial resistance (Rt) followed exposure to PGD2. Indomethacin had no significant effect on Rt or Ieq, but exposure of indomethacin-pretreated preparations to PGE2 revealed a minor (2%) increase in Ieq. In contrast, tracheal cell epithelia exhibited significant changes in Rt and Ieq in response to PGF2 alpha, PGE2, and HHT. These results indicate that Clara cells metabolize arachidonic acid to biologically active eicosanoids, but the resulting products do not play a major role in regulation of transepithelial ion transport by this cell type.

  2. Spatially explicit West Nile virus risk modeling in Santa Clara County, California

    Science.gov (United States)

    A previously created Geographic Information Systems model designed to identify regions of West Nile virus (WNV) transmission risk is tested and calibrated in Santa Clara County, California. American Crows that died from WNV infection in 2005 provide the spatial and temporal ground truth. Model param...

  3. Spatially Explicit West Nile Virus Risk Modeling in Santa Clara County, CA

    Science.gov (United States)

    A geographic information systems model designed to identify regions of West Nile virus (WNV) transmission risk was tested and calibrated with data collected in Santa Clara County, California. American Crows that died from WNV infection in 2005, provided spatial and temporal ground truth. When the mo...

  4. Ciliate Paramecium is a natural reservoir of Legionella pneumophila

    Science.gov (United States)

    Watanabe, Kenta; Nakao, Ryo; Fujishima, Masahiro; Tachibana, Masato; Shimizu, Takashi; Watarai, Masahisa

    2016-04-01

    Legionella pneumophila, the causative agent of Legionnaires’ disease, replicates within alveolar macrophages and free-living amoebae. However, the lifestyle of L. pneumophila in the environment remains largely unknown. Here we established a novel natural host model of L. pneumophila endosymbiosis using the ciliate Paramecium caudatum. We also identified Legionella endosymbiosis-modulating factor A (LefA), which contributes to the change in life stage from endosymbiosis to host lysis, enabling escape to the environment. We isolated L. pneumophila strains from the environment, and they exhibited cytotoxicity toward P. caudatum and induced host lysis. Acidification of the Legionella-containing vacuole (LCV) was inhibited, and enlarged LCVs including numerous bacteria were observed in P. caudatum infected with L. pneumophila. An isogenic L. pneumophila lefA mutant exhibited decreased cytotoxicity toward P. caudatum and impaired the modification of LCVs, resulting in the establishment of endosymbiosis between them. Our results suggest that L. pneumophila may have a mechanism to switch their endosymbiosis in protistan hosts in the environment.

  5. The mitochondrial genomes of the ciliates Euplotes minuta and Euplotes crassus

    Directory of Open Access Journals (Sweden)

    Huynh Minh

    2009-11-01

    Full Text Available Abstract Background There are thousands of very diverse ciliate species from which only a handful mitochondrial genomes have been studied so far. These genomes are rather similar because the ciliates analysed (Tetrahymena spp. and Paramecium aurelia are closely related. Here we study the mitochondrial genomes of the hypotrichous ciliates Euplotes minuta and Euplotes crassus. These ciliates are only distantly related to Tetrahymena spp. and Paramecium aurelia, but more closely related to Nyctotherus ovalis, which possesses a hydrogenosomal (mitochondrial genome. Results The linear mitochondrial genomes of the hypotrichous ciliates Euplotes minuta and Euplotes crassus were sequenced and compared with the mitochondrial genomes of several Tetrahymena species, Paramecium aurelia and the partially sequenced mitochondrial genome of the anaerobic ciliate Nyctotherus ovalis. This study reports new features such as long 5'gene extensions of several mitochondrial genes, extremely long cox1 and cox2 open reading frames and a large repeat in the middle of the linear mitochondrial genome. The repeat separates the open reading frames into two blocks, each having a single direction of transcription, from the repeat towards the ends of the chromosome. Although the Euplotes mitochondrial gene content is almost identical to that of Paramecium and Tetrahymena, the order of the genes is completely different. In contrast, the 33273 bp (excluding the repeat region piece of the mitochondrial genome that has been sequenced in both Euplotes species exhibits no difference in gene order. Unexpectedly, many of the mitochondrial genes of E. minuta encoding ribosomal proteins possess N-terminal extensions that are similar to mitochondrial targeting signals. Conclusion The mitochondrial genomes of the hypotrichous ciliates Euplotes minuta and Euplotes crassus are rather different from the previously studied genomes. Many genes are extended in size compared to mitochondrial

  6. Origins of learned reciprocity in solitary ciliates searching grouped 'courting' assurances at quantum efficiencies.

    Science.gov (United States)

    Clark, Kevin B

    2010-01-01

    Learning to reciprocate socially valued actions, such as cheating and cooperation, marks evolutionary advances in animal intelligence thought unequalled by even colonial microbes known to secure respective individual or group fitness tradeoffs through genetic and epigenetic processes. However, solitary ciliates, unique among microbes for their emulation of simple Hebbian-like learning contingent upon feedback between behavioral output and vibration-activated mechanosensitive Ca(2+) channels, might be the best candidates to learn to reciprocate necessary preconjugant touches perceived during complex 'courtship rituals'. Testing this hypothesis here with mock social trials involving an ambiguous vibration source, the large heterotrich ciliate Spirostomum ambiguum showed it can indeed learn to modify emitted signals about mating fitness to encourage paired reproduction. Ciliates, improving their signaling expertise with each felt vibration, grouped serial escape strategies gesturing opposite 'courting' assurances of playing 'harder to get' or 'easier to get' into separate, topologically invariant computational networks. Stored strategies formed patterns of action or heuristics with which ciliates performed fast, quantum-like distributed modular searches to guide future replies of specific fitness content. Heuristic-guided searches helped initial inferior repliers, ciliates with high initial reproductive costs, learn to sensitize their behavioral output and opportunistically compete with presumptive mating 'rivals' advertising higher quality fitness. Whereas, initial superior repliers, ciliates with low initial reproductive costs, learned with the aid of heuristics to habituate their behavioral output and sacrifice net reproductive payoffs to cooperate with presumptive 'suitors', a kind of learned altruism only before attributed to animal social intelligences. The present findings confirm that ciliates are highly competent decision makers capable of achieving paired

  7. Involvement of Crawling and Attached Ciliates in the Aggregation of Particles in Wastewater Treatment Plants

    Directory of Open Access Journals (Sweden)

    Lucía Arregui

    2008-01-01

    Full Text Available The biological community in activated sludge wastewater plants is organized within this ecosystem as bioaggregates or flocs, in which the biotic component is embedded in a complex matrix comprised of extracellular polymeric substances mainly of microbial origin. The aim of this work is to study the role of different floc-associated ciliates commonly reported in wastewater treatment plants-crawling Euplotes and sessile Vorticella- in the formation of aggregates. Flocs, in experiments with ciliates and latex beads, showed more compactation and cohesion among particles than those in the absence of ciliates. Ciliates have been shown to contribute to floc formation through different mechanisms such as the active secretion of polymeric substances (extrusomes, their biological activities (movement and feeding strategies, or the cysts formation capacity of some species. Staining with lectins coupled to fluorescein showed that carbohydrate of the matrix contained glucose, manose, N-acetyl-glucosamine and galactose. Protein fraction revealed over the latex beads surfaces could probably be of bacterial origin, but nucleic acids represented an important fraction of the extracellular polymeric substances of ciliate origin.

  8. The D1-D2 region of the large subunit ribosomal DNA as barcode for ciliates.

    Science.gov (United States)

    Stoeck, T; Przybos, E; Dunthorn, M

    2014-05-01

    Ciliates are a major evolutionary lineage within the alveolates, which are distributed in nearly all habitats on our planet and are an essential component for ecosystem function, processes and stability. Accurate identification of these unicellular eukaryotes through, for example, microscopy or mating type reactions is reserved to few specialists. To satisfy the demand for a DNA barcode for ciliates, which meets the standard criteria for DNA barcodes defined by the Consortium for the Barcode of Life (CBOL), we here evaluated the D1-D2 region of the ribosomal DNA large subunit (LSU-rDNA). Primer universality for the phylum Ciliophora was tested in silico with available database sequences as well as in the laboratory with 73 ciliate species, which represented nine of 12 ciliate classes. Primers tested in this study were successful for all tested classes. To test the ability of the D1-D2 region to resolve conspecific and congeneric sequence divergence, 63 Paramecium strains were sampled from 24 mating species. The average conspecific D1-D2 variation was 0.18%, whereas congeneric sequence divergence averaged 4.83%. In pairwise genetic distance analyses, we identified a D1-D2 sequence divergence of barcode for ciliated protists. © 2013 John Wiley & Sons Ltd.

  9. Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England

    Directory of Open Access Journals (Sweden)

    Sarah J. Tucker

    2017-11-01

    Full Text Available Despite their important role of linking microbial and classic marine food webs, data on biogeographical patterns of microbial eukaryotic grazers are limited, and even fewer studies have used molecular tools to assess active (i.e., those expressing genes community members. Marine ciliate diversity is believed to be greatest at the chlorophyll maximum, where there is an abundance of autotrophic prey, and is often assumed to decline with depth. Here, we assess the abundant (DNA and active (RNA marine ciliate communities throughout the water column at two stations off the New England coast (Northwest Atlantic—a coastal station 43 km from shore (40 m depth and a slope station 135 km off shore (1,000 m. We analyze ciliate communities using a DNA fingerprinting technique, Denaturing Gradient Gel Electrophoresis (DGGE, which captures patterns of abundant community members. We compare estimates of ciliate communities from SSU-rDNA (abundant and SSU-rRNA (active and find complex patterns throughout the water column, including many active lineages below the photic zone. Our analyses reveal (1 a number of widely-distributed taxa that are both abundant and active; (2 considerable heterogeneity in patterns of presence/absence of taxa in offshore samples taken 50 m apart throughout the water column; and (3 three distinct ciliate assemblages based on position from shore and depth. Analysis of active (RNA taxa uncovers biodiversity hidden to traditional DNA-based approaches (e.g., clone library, rDNA amplicon studies.

  10. Morphological and molecular characterization of renal ciliates infecting farmed snails in Spain.

    Science.gov (United States)

    Segade, P; Kher, C P; Lynn, D H; Iglesias, R

    2009-06-01

    Renal infections by parasitic ciliates were studied in adult snails of Helix aspersa aspersa and Helix aspersa maxima collected from 2 mixed rearing system-based heliciculture farms located in Galicia (NW Spain). The occurrence of ciliates was also examined in slugs (Deroceras reticulatum) invading the greenhouses where first growing and fattening of snails is carried out. Histological examinations revealed a severe destruction of the renal epithelium in heavily infected hosts. Three ciliate isolates, one from each host species, were obtained and grown in axenic cultures. Cultured and parasitic ciliates were characterized morphologically and morphometrically. In addition, the encystment behaviour, the occurrence of autogamy, and the sequences of the mitochondrial cytochrome-c oxidase subunit 1 (cox1) and the small subunit ribosomal RNA (SSU rRNA) genes were also studied in the 3 isolates. A polymorphic life cycle involving resting and reproductive cysts, together with the morphological and morphometrical characteristics and the confirmation that autogamy occurs within cysts, demonstrate that our ciliates belong to the species Tetrahymena rostrata (Kahl, 1926) Corliss, 1952. The 3 isolates formed a well-supported clade using both genetic markers, and were clearly separate from the strain ATCC(R) 30770, which has been identified as Tetrahymena rostrata. We argue that our Spanish isolates should be regarded as Tetrahymena rostrata, and that the ATCC isolate should be regarded as a misidentification as neither cytological nor cytogenetical support for its identity has been presented.

  11. Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells.

    Science.gov (United States)

    Pinheiro, Marcel D O; Bols, Niels C

    2014-10-01

    Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant for phagocytosis) were cultured on monolayers of 3 fish epithelial cell lines, CHSE-214, RTgill-W1, and ZEB2J, and the rabbit kidney epithelial cell line, RK-13. Generally the ciliates flourished, whereas the monolayers died, being completely consumed over several days. The destruction of monolayers required that the ciliates could make contact with the animal cells through swimming, which appeared to dislodge or loosen cells so that they could be phagocytosed. The ciliates internalized into food vacuoles ZEB2J from cell monolayers as well as from cell suspensions. Phagocytosis was essential for monolayer destruction as monolayers remained intact under conditions where phagocytosis was impeded, such as 37°C for NP1 and 4°C for B1975. Monolayers of fish cells supported the proliferation of ciliates. Thus T. thermophila can 'eat' animal cells or be histophagous in vitro, with the potential to be histophagous in vivo. © 2014 International Federation for Cell Biology.

  12. Diversity of Oligotrichia and Choreotrichia Ciliates in Coastal Marine Sediments and in Overlying Plankton▿

    Science.gov (United States)

    Doherty, Mary; Tamura, Maiko; Vriezen, Jan A. C.; McManus, George B.; Katz, Laura A.

    2010-01-01

    Elucidating the relationship between ciliate communities in the benthos and the plankton is critical to understanding ciliate diversity in marine systems. Although data for many lineages are sparse, at least some members of the dominant marine ciliate clades Oligotrichia and Choreotrichia can be found in both plankton and benthos, in the latter either as cysts or active forms. In this study, we developed a molecular approach to address the relationship between the diversity of ciliates in the plankton and those of the underlying benthos in the same locations. Samples from plankton and sediments were compared across three sites along the New England coast, and additional subsamples were analyzed to assess reproducibility of methods. We found that sediment and plankton subsamples differed in their robustness to repeated subsampling. Sediment subsamples (i.e., 1-g aliquots from a single ∼20-g sample) gave variable estimates of diversity, while plankton subsamples produced consistent results. These results indicate the need for additional study to determine the spatial scale over which diversity varies in marine sediments. Clustering of phylogenetic types indicates that benthic assemblages of oligotrichs and choreotrichs appear to be more like those from spatially remote benthic communities than the ciliate communities sampled in the water above them. PMID:20435761

  13. Diversity of oligotrichia and choreotrichia ciliates in coastal marine sediments and in overlying plankton.

    Science.gov (United States)

    Doherty, Mary; Tamura, Maiko; Vriezen, Jan A C; McManus, George B; Katz, Laura A

    2010-06-01

    Elucidating the relationship between ciliate communities in the benthos and the plankton is critical to understanding ciliate diversity in marine systems. Although data for many lineages are sparse, at least some members of the dominant marine ciliate clades Oligotrichia and Choreotrichia can be found in both plankton and benthos, in the latter either as cysts or active forms. In this study, we developed a molecular approach to address the relationship between the diversity of ciliates in the plankton and those of the underlying benthos in the same locations. Samples from plankton and sediments were compared across three sites along the New England coast, and additional subsamples were analyzed to assess reproducibility of methods. We found that sediment and plankton subsamples differed in their robustness to repeated subsampling. Sediment subsamples (i.e., 1-g aliquots from a single approximately 20-g sample) gave variable estimates of diversity, while plankton subsamples produced consistent results. These results indicate the need for additional study to determine the spatial scale over which diversity varies in marine sediments. Clustering of phylogenetic types indicates that benthic assemblages of oligotrichs and choreotrichs appear to be more like those from spatially remote benthic communities than the ciliate communities sampled in the water above them.

  14. Multiple genes of apparent algal origin suggest ciliates may once have been photosynthetic.

    Science.gov (United States)

    Reyes-Prieto, Adrian; Moustafa, Ahmed; Bhattacharya, Debashish

    2008-07-08

    Plantae (as defined by Cavalier-Smith, 1981) plastids evolved via primary endosymbiosis whereby a heterotrophic protist enslaved a photosynthetic cyanobacterium. This "primary" plastid spread into other eukaryotes via secondary endosymbiosis. An important but contentious theory in algal evolution is the chromalveolate hypothesis that posits chromists (cryptophytes, haptophytes, and stramenopiles) and alveolates (ciliates, apicomplexans, and dinoflagellates) share a common ancestor that contained a red-algal-derived "secondary" plastid. Under this view, the existence of several later-diverging plastid-lacking chromalveolates such as ciliates and oomycetes would be explained by plastid loss in these lineages. To test the idea of a photosynthetic ancestry for ciliates, we used the 27,446 predicted proteins from the macronuclear genome of Tetrahymena thermophila to query prokaryotic and eukaryotic genomes. We identified 16 proteins of possible algal origin in the ciliates Tetrahymena and Paramecium tetraurelia. Fourteen of these are present in other chromalveolates. Here we compare and contrast the likely scenarios for algal-gene origin in ciliates either via multiple rounds of horizontal gene transfer (HGT) from algal prey or symbionts, or through endosymbiotic gene transfer (EGT) during a putative photosynthetic phase in their evolution.

  15. Efeito de reguladores de crescimento em uva apirênica, cv. BRS Clara Effect of growth regulators on the seedless grape cv. BRS Clara

    Directory of Open Access Journals (Sweden)

    Jair Costa Nachtigal

    2005-08-01

    Full Text Available O presente trabalho teve por objetivo verificar o efeito da aplicação de diferentes concentrações do ácido giberélico (AG3, do thidiazuron (TDZ e do forchlorfenuron (CPPU no aumento do tamanho dos cachos e das bagas e no teor de sólidos solúveis totais de uva sem semente, cv. BRS Clara. As plantas foram conduzidas no sistema de latada, sobre o porta-enxerto IAC 572, no espaçamento 2,5 x 2,0m e com irrigação por microaspersão. Os experimentos foram conduzidos na Estação Experimental de Viticultura Tropical, da Embrapa Uva e Vinho, em Jales-SP. Os reguladores de crescimento foram aplicados via pulverização localizada no cacho, utilizando-se de concentrações de 0 a 4mg.L-1 de CPPU; 0 a 10mg.L-1 de TDZ, e de 0 a 90mg.L-1 de AG3, isolados ou em conjunto. De modo geral, a aplicação dos reguladores de crescimento promove a melhoria da qualidade dos cachos da cv. BRS Clara; o uso do TDZ e do CPPU em conjunto com o AG3 produz um efeito sinérgico, proporcionando melhor resposta do que o uso isolado do AG3; os tratamentos com 60mg.L-1 de AG3; 20mg.L-1 de AG3 + 4mg.L-1 de CPPU, e 10mg.L-1 de AG3 + 5mg.L-1 de TDZ proporcionam os melhores resultados para o aumento do diâmetro das bagas; a aplicação de AG3 antes da floração da cv. BRS Clara provoca abortamento excessivo, reduzindo a qualidade comercial dos cachos; a utilização de concentrações elevadas dos reguladores reduz o teor de sólidos solúveis totais.The objective of this study was to evaluate the effect of different concentrations of gibberellic acid (AG3, thidiazuron (TDZ and forchlorfenuron (CPPU on the size of bunches and berries and on the total soluble solids content, when applied in different concentrations on the seedless grapes, cv. BRS Clara. The grapevines were grafted on the rootstock IAC 572, trained in the pergola system and, spaced 2.5 x 2.0m and irrigated by micro sprinklers. The experiments were carried out at the Embrapa Grape and Wine Experimental

  16. Physical subdivision and description of the water-bearing sediments of the Santa Clara Valley, California

    Science.gov (United States)

    Wentworth, Carl M.; Jachens, Robert C.; Williams, Robert A.; Tinsley, John C.; Hanson, Randall T.

    2015-01-01

    A thick Quaternary alluvial section fills a sedimentary basin beneath the Santa Clara Valley, California, located within the San Andreas Fault system at the south end of San Francisco Bay. This section consists of an upper sequence about 1,000 feet thick containing eight sedimentary cycles and a lower fine-grained unit as thick as several hundred feet. Together these constitute the Quaternary Santa Clara Basin. The section overlies an irregular unconformity with more than 1,200 feet of relief cut into the underlying bedrock. This stratigraphy is determined through study of new wells and seismic reflection profiles, together with a sample of the many thousands of water wells in the valley. It represents a major change and improvement in understanding of the basin, particularly with regard to the upper cyclic sequence, which forms a large groundwater system that is an important resource in the San Francisco Bay region.

  17. Estimation of carbon sequestration by tree species in three farms in Villa Clara province

    Directory of Open Access Journals (Sweden)

    José Carlos García Hernández

    2016-03-01

    Full Text Available The research took place in two farms of the Cifuentes municipality and in a farm of the Santa Clara municipality, province of Villa Clara. It was carried out from November 2008 to May 2009, with the objective of estimating the capture of Carbon by common arboreal species in these farms. The plant biomass analyses were made according to the methodology of Mercadet and Álvarez (2005. Several parameters were analyzed. Age, height and perimeter of the plants were the most influential parameters on the amount of carbon sequestered. In this regard, we performed a multivariate curve fitting, showing the age of the plants the highest percent of dependence (54.89%. Palma Real with values between 0.81 and 0.84 t / plant and the Mango between 0.60 and 0.81 t /plant of retained Carbon, were the most outstanding in the capture of Carbon.

  18. Rumen ciliate protozoa of domestic sheep (Ovis aries) and goat (Capra aegagrus hircus) in Kyrgyzstan.

    Science.gov (United States)

    Gürelli, Gözde; Canbulat, Savaş; Aldayarov, Nurbek; Dehority, Burk A

    2016-03-01

    Species composition and concentration of rumen ciliate protozoa were investigated in the rumen contents of 14 domestic sheep and 1 goat living in Bishkek, Kyrgyzstan. This is the first report on rumen ciliates from ruminants living in Kyrgyzstan. In sheep 12 genera, 28 species and 12 morphotypes were detected, whereas in goat 8 genera, 12 species and 4 morphotypes were detected. The density of ciliates in sheep was (28.1 ± 20.0) × 10(4) cells mL(-1) and in goat was 37.0 × 10(4) cells mL(-1). Dasytricha ruminantium, Isotricha prostoma, Entodinium simulans and Ophryoscolex caudatus were major species (100%) in sheep, and for the first time, Diplodinium rangiferi was detected in a domestic goat. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  19. Evolution of germline-limited sequences in two populations of the ciliate Chilodonella uncinata.

    Science.gov (United States)

    Zufall, Rebecca A; Sturm, Mariel; Mahon, Brian C

    2012-04-01

    Ciliates are microbial eukaryotes that separate their nuclear functions into a germline micronucleus and a somatic macronucleus. During development of the macronucleus the genome undergoes a series of reorganization events that includes the precise excision of intervening DNA. Here, we determine the architecture of four loci in the micronuclear and macronuclear genomes of the ciliate Chilodonella uncinata and compare the levels of variation in micronuclear-limited sequences to macronuclear destined sequences at two of these loci. We find that within a population, germline-limited sequences are evolving at the same rate as other putatively neutral sites, but between populations germline-limited sequences are accumulating mutations at a much faster rate than other sites. We also find evidence of macronuclear recombination and incomplete elimination of intervening DNA, which result in increased diversity in the macronuclear genome. Our results support the assertion that the unusual genomic features of ciliates can result in rapid and unpredicted patterns of diversification.

  20. Elimination of IL-13 Reverses Established Goblet Cell Metaplasia into Ciliated Epithelia in Airway Epithelial Cell Culture

    Directory of Open Access Journals (Sweden)

    Mitsuko Kondo

    2006-01-01

    Conclusions: Elimination of IL-13 reverses goblet cell metaplasia into ciliated epithelia in vitro, and transition of goblet cells to other phenotypes, especially ciliated cells, may be involved in this phenomenon. IL-13 inhibition may be a therapeutic strategy of established goblet cell metaplasia in asthma.

  1. Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment

    NARCIS (Netherlands)

    Ricard, G.; McEwan, N.R.; Dutilh, B.E.; Jouany, J.P.; Macheboeuf, D.; Mitsumori, M.; McIntosh, F.M.; Michalowski, T.; Nagamine, T.; Nelson, N.; Newbold, C.J.; Nsabimana, E.; Takenaka, A.; Thomas, N.A.; Ushida, K.; Hackstein, J.H.P.; Huynen, M.A.

    2006-01-01

    BACKGROUND: The horizontal transfer of expressed genes from Bacteria into Ciliates which live in close contact with each other in the rumen (the foregut of ruminants) was studied using ciliate Expressed Sequence Tags (ESTs). More than 4000 ESTs were sequenced from representatives of the two major

  2. Recent results in multinucleon transfer reactions studied with PRISMA+CLARA

    CERN Document Server

    Corradi, L

    2010-01-01

    With the large solid angle magnetic spectrometer PRISMA coupled to the array CLARA extensive investigations have been carried out for nuclear structure and reaction dynamics. In the present paper aspects of these studies will be presented, focusing more closely on the reaction mechanism, in particular on the properties of quasi-elastic and deep-inelastic processes and on measurements at energies far below the Coulomb barrier.

  3. Deep-sea ciliates: Recorded diversity and experimental studies on pressure tolerance

    Science.gov (United States)

    Schoenle, Alexandra; Nitsche, Frank; Werner, Jennifer; Arndt, Hartmut

    2017-10-01

    Microbial eukaryotes play an important role in biogeochemical cycles not only in productive surface waters but also in the deep sea. Recent studies based on metagenomics report deep-sea protistan assemblages totally different from continental slopes and shelf waters. To give an overview about the ciliate fauna recorded from the deep sea we summarized the available information on ciliate occurrence in the deep sea. Our literature review revealed that representatives of the major phylogenetic groups of ciliates were recorded from the deep sea (> 1000 m depth): Karyorelictea, Heterotrichea, Spirotrichea (Protohypotrichia, Euplotia, Oligotrichia, Choreotrichia, Hypotrichia), Armophorea (Armophorida), Litostomatea (Haptoria), Conthreep (Phyllopharyngea incl. Cyrtophoria, Chonotrichia, Suctoria; Nassophorea incl. Microthoracida, Synhymeniida, Nassulida; Colpodea incl. Bursariomorphida, Cyrtolophosidida; Prostomatea; Plagiopylea incl. Plagiopylida, Odontostomatida; Oligohymenophorea incl. Peniculia, Scuticociliatia, Hymenostomatia, Apostomatia, Peritrichia, Astomatia). Species occurring in both habitats, deep sea and shallow water, are rarely found to our knowledge to date. This indicates a high deep-sea specific ciliate fauna. Our own studies of similar genotypes (SSU rDNA and cox1 gene) revealed that two small scuticociliate species (Pseudocohnilembus persalinus and Uronema sp.) could be isolated from surface as well as deep waters (2687 m, 5276 m, 5719 m) of the Pacific. The adaptation to deep-sea conditions was investigated by exposing the ciliate isolates directly or stepwise to different hydrostatic pressures ranging from 1 to 550 atm at temperatures of 2 °C and 13 °C. Although the results indicated no general barophilic behavior, all four isolated strains survived the highest established pressure. A better survival at 550 atm could be observed for the lower temperature. Among microbial eukaryotes, ciliates should be considered as a diverse and potentially

  4. Short-term Influence of Drilling Fluid on Ciliates from Activated Sludge in Sequencing Batch Reactors.

    Science.gov (United States)

    Babko, Roman; Kuzmina, Tatiana; Łagód, Grzegorz; Jaromin-Gleń, Katarzyna; Danko, Yaroslav; Pawłowska, Małgorzata; Pawłowski, Artur

    2017-01-01

    Spent drilling muds are the liquid residues of rock drilling operations. Due to a high concentration of suspended solids and potentially detrimental chemical properties, they can negatively affect microorganisms participating in wastewater treatment processes. We evaluated the addition of a potassium-polymer drilling fluid (DF) to activated sludge in laboratory sequencing batch reactors (SBRs) for municipal wastewater treatment. Ciliate assemblage, the most dynamic component of eukaryotes in activated sludge, and which is highly sensitive to changes in the system, was evaluated. The average ciliate abundance dropped by about 51% (SBR 2; 1% DF added) and 33% (SBR 3; 3% DF added) in comparison to the control (SBR 1; wastewater only). A decrease in the total number of ciliate species during the experiment was observed, from 25 to 24 in SBR 2 and from 17 to 13 in SBR 3. Moreover, a drop in the number of dominant (>100 individuals mL) ciliate species was observed during the experiment-from eight in the control to five in SBR 2 and four in SBR 3-signaling noticeable changes in the quantitative structure of ciliate species. The species analyzed showed different responses to DF addition. The most sensitive was , which is bacteriovorus. In contrast, two predators, and , showed no reaction to DF addition. Our results indicate that addition of potassium-polymer DF, in doses of 1 to 3% of the treated wastewater volume, had no toxic effects on ciliates, but qualitative and quantitative changes in their community were observed. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  5. Rare Freshwater Ciliate Paramecium chlorelligerum Kahl, 1935 and Its Macronuclear Symbiotic Bacterium "Candidatus Holospora parva".

    Directory of Open Access Journals (Sweden)

    Olivia Lanzoni

    Full Text Available Ciliated protists often form symbioses with many diverse microorganisms. In particular, symbiotic associations between ciliates and green algae, as well as between ciliates and intracellular bacteria, are rather wide-spread in nature. In this study, we describe the complex symbiotic system between a very rare ciliate, Paramecium chlorelligerum, unicellular algae inhabiting its cytoplasm, and novel bacteria colonizing the host macronucleus. Paramecium chlorelligerum, previously found only twice in Germany, was retrieved from a novel location in vicinity of St. Petersburg in Russia. Species identification was based on both classical morphological methods and analysis of the small subunit rDNA. Numerous algae occupying the cytoplasm of this ciliate were identified with ultrastructural and molecular methods as representatives of the Meyerella genus, which before was not considered among symbiotic algae. In the same locality at least fifteen other species of "green" ciliates were found, thus it is indeed a biodiversity hot-spot for such protists. A novel species of bacterial symbionts living in the macronucleus of Paramecium chlorelligerum cells was morphologically and ultrastructurally investigated in detail with the description of its life cycle and infection capabilities. The new endosymbiont was molecularly characterized following the full-cycle rRNA approach. Furthermore, phylogenetic analysis confirmed that the novel bacterium is a member of Holospora genus branching basally but sharing all characteristics of the genus except inducing connecting piece formation during the infected host nucleus division. We propose the name "Candidatus Holospora parva" for this newly described species. The described complex system raises new questions on how these microorganisms evolve and interact in symbiosis.

  6. Power conversion and quality of the Santa Clara 2 MW direct carbonate fuel cell demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    Skok, A.J. [Fuel Cell Engineering Corp., Danbury, CT (United States); Abueg, R.Z. [Basic Measuring Instruments, Santa Clara, CA (United States); Schwartz, P. [Fluor Daniel, Inc., Irvine, CA (United States)] [and others

    1996-12-31

    The Santa Clara Demonstration Project (SCDP) is the first application of a commercial-scale carbonate fuel cell power plant on a US electric utility system. It is also the largest fuel cell power plant ever operated in the United States. The 2MW plant, located in Santa Clara, California, utilizes carbonate fuel cell technology developed by Energy Research Corporation (ERC) of Danbury, Connecticut. The ultimate goal of a fuel cell power plant is to deliver usable power into an electrical distribution system. The power conversion sub-system does this for the Santa Clara Demonstration Plant. A description of this sub-system and its capabilities follows. The sub-system has demonstrated the capability to deliver real power, reactive power and to absorb reactive power on a utility grid. The sub-system can be operated in the same manner as a conventional rotating generator except with enhanced capabilities for reactive power. Measurements demonstrated the power quality from the plant in various operating modes was high quality utility grade power.

  7. Carcinoma odontogénico de células claras

    Directory of Open Access Journals (Sweden)

    Sergio Alberto Flores Alvarado

    2011-03-01

    Full Text Available El carcinoma odontogénico de células claras es un raro tumor odontogénico que se presenta principalmente en la parte anterior de la mandíbula, mayormente en mujeres entre la quinta y séptima década de vida. Se caracteriza histológicamente por redes de células con citoplasma claro mezcladas con células que contienen citoplasma eosinófilo. Es sumamente agresivo y puede presentar metástasis local o a distancia. Al emplearse el tratamiento por enucleación y curetaje, frecuentemente da lugar a recurrencia, por lo que se aconseja el tratamiento radical de resección mandibular hasta una zona libre de lesión. El carcinoma odontogénico de células claras debe ser considerado en el diagnóstico diferencial de tumores mandibulares que presenten células claras para el establecimiento del plan de tratamiento adecuado. Se debe incluir el seguimiento a largo plazo del paciente. Se presenta el caso de un paciente femenino de 69 años de edad, con una lesión presente en la región presinfisaria derecha, sin afectación de ganglios linfáticos regionales o metástasis aparente. Se efectuó osteotomía segmentaria mandibular y reconstrucción con placa de titanio.

  8. Evaluation of animal control measures on pet demographics in Santa Clara County, California, 1993–2006

    Directory of Open Access Journals (Sweden)

    Philip H. Kass

    2013-02-01

    Full Text Available The measurable benefits of animal control programs are unknown and the aim of this study was to determine the impact of these programs on pet population changes. A prospective cross-sectional study of 1000 households was implemented in 2005 to evaluate characteristics of the owned and unowned population of dogs and cats in Santa Clara County, California. The same population was previously studied 12 years earlier. During this time period, the county instituted in 1994 and then subsequently disestablished a municipal spay/neuter voucher program for cats. Dog intakes declined from 1992–2005, as they similarly did for an adjacent county (San Mateo. However, cat intakes declined significantly more in Santa Clara County than San Mateo, with an average annual decline of approximately 700 cats for the 12 year period. Time series analysis showed a greater than expected decline in the number of cats surrendered to shelters in Santa Clara County during the years the voucher program was in effect (1994–2005. The net savings to the county by reducing the number of cat shelter intakes was estimated at approximately $1.5 million. The measurable benefits of animal control programs are unknown and the aim of this study was to determine the impact of these programs on pet population changes.

  9. Initial steps of infection of the ciliate Paramecium with bacteria Holospora sp.

    Science.gov (United States)

    Fokin, S I; Skovorodkin, I N; Sabaneyeva, E V

    2003-01-01

    New light and electron microscope data on the initial steps of endocytobiosis establishment between the ciliate Paramecium and specific intranuclear bacteria Holospora are provided. At the cytoplasmic step of infection bacteria of all Holospora species are found in a vesicle originating from the membrane of the host cell phagosome. The association between host cell microfilaments and the bacterium bearing vesicle may suggest a possible involvement of the ciliate cytoskeleton in the transportation of bacteria to the host cell nucleus. The authors subdivide the process of infection into 6 steps. Some strains of P. caudatum never take up infectious Holospora bacteria in the course of phagocytosis.

  10. Hyperparasitism of trichodinid ciliates on monogenean gill flukes of two marine fish.

    Science.gov (United States)

    Colorni, A; Diamant, A

    2005-06-01

    Two unusual cases of hyperparasitism of trichodinid ciliates on monogenean gill flukes are described from southern Israel (Red Sea). The first case occurred in cultured European sea bass Dicentrarchus labrax infected by Diplectanum aequans, while the second was observed in a feral devil firefish Pterois miles infected by Haliotrema sp. In both cases, the trichodinids heavily co-infested the host fish gills. The flukes were completely coated by the ciliates, which gave them a cobblestone appearance, but no damage to their tegument was apparent. Both cases are most likely a result of accidental hyperparasitism, brought about by perturbed environmental conditions.

  11. Free-Living Marine Interstitial Hypotrichid Ciliates from Jubail Marine Wildlife Sanctuary in the Arabian Gulf

    Directory of Open Access Journals (Sweden)

    K.A.S. AL-Rasheid

    1999-01-01

    Full Text Available Sediment samples were collected at low tide from various localities of the Jubail Marine Wildlife Sanctuary in the Arabian Gulf on several occasions during l996-l997 for the study of the marine interstitial ciliate fauna of the Sanctuary. Twenty three species belonging to the order Hypotrichida were identified after protargol impregnation, 20 of which represent new records of the fauna of Saudi Arabia, and of the Arabian Gulf at large. The distribution of each species is compared to those in similar habitats worldwide. The present study increases the total known number of hypotrichid ciliates species in Saudi Arabia to 40 species.

  12. Thrust Faulting, Vp, Vs, Vp/Vs and Poisson's Ratios Beneath the Santa Clara Valley, California as Determined from the Santa Clara Seismic Investigation (SCSI)

    Science.gov (United States)

    Catchings, R. D.; Goldman, M. R.; Kiger, L.; Saulter, D.; Wentworth, C.

    2001-12-01

    In December 2000, we conducted an ~20-km-long, southwest-northeast-trending seismic reflection and refraction seismic imaging survey extending from the central Santa Cruz Mountains to the central Santa Clara Valley. The Santa Clara Seismic investigation (SCSI) originated within 0.5 km of the surface trace of the San Andreas fault zone and terminated in downtown San Jose. Between its origin and terminus, the profile crossed the Cupertino Basin and numerous mapped faults, including the Monte Vista thrust fault. Seismic sources were generated by 11 explosions ranging in size from about 12 to 23 kg (25 to 50 lbs.) and were spaced approximately 1 km apart in the central part of the profile. The seismic data were recorded with approximately 400 PASSCAL Texan seismographs, spaced approximately 50 m apart. We inverted first-arrival refractions to determine the P- and S-wave velocity structure of the upper 3.5 km of the crust, and we stacked the seismic reflection data to develop reflection images the upper 10 km of the crust. Our data indicate that P-wave velocities range from about 1.3 km/s near the surface to about 6.0 km/s at varying depths along the profile. Measured S-wave velocities range from about 0.6 km/s near the surface to about 2.6 km/s at varying depths along the profile. Vp/Vs and Poisson's ratios vary from about 1.57 to about 2.10 and from 0.16 to about 0.37, respectively. All velocities and ratios vary laterally and vertically along the profile, but there is an appreciable difference on either side of the Monte Vista fault. Beneath the central Cupertino Basin, the velocity data suggest that sediments (3 km/s) are about 1.2 km deep, thinning to about 600 m deep beneath downtown San Jose and to 0 km deep in the Santa Cruz Mountains. These data show that the Santa Clara Valley and Cupertino Basin sediments are shallower beneath our profile than previously believed. The velocity image also shows a clear low-velocity zone that is most prominent beneath the town

  13. Conspicuous peak of oligotrichous ciliates following winter stratification in a bog lake

    Czech Academy of Sciences Publication Activity Database

    Macek, Miroslav; Šimek, Karel; Bittl, T.

    2001-01-01

    Roč. 23, č. 4 (2001), s. 353-363 ISSN 0142-7873 R&D Projects: GA ČR GA206/99/0028; GA AV ČR KSK2005601 Keywords : ciliates * feeding rates * ice Subject RIV: EH - Ecology, Behaviour Impact factor: 1.259, year: 2001

  14. Light microscopic morphometrics, ultrastructure, and molecular phylogeny of the putative pycnotrichid Ciliate, Buxtonella sulcata.

    Science.gov (United States)

    Norman Grim, J; Jirků-Pomajbíková, Kateřina; Ponce-Gordo, Francisco

    2015-10-01

    The ciliate, Buxtonella sulcata, was isolated from a bull cow near Tišnov, Czech Republic, and fixed for light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) study. Presented here are the basic morphometrics from LM study, and the fine-structure of both somatic and vestibular ciliary, and other structures. While many morphological features are similar to ciliates belonging to the order Vestibuliferida, some differences have been discovered, and are presented here. Especially emphasized are the microtubular and fibrilar components of the basic kinetid structures for both somatic and vestibular regions of these protists. Also observed in both TEM and SEM samples were enigmatic membrane bulges at the base of many somatic cilia. These ciliates are seen to have abundant endocytoplasmic bacteria, as seen in LM and TEM. This evaluation of the ultrastructural morphology of B. sulcata from cattle is accompanied by detailed determination of its small subunit rRNA (SSU rRNA) gene sequence and also of internal transcribed spacers (ITS1-5.8rRNA-ITS2). All of these data will contribute to unravel the phylogenetic relationships of medically and veterinary important intestinal ciliates. Copyright © 2015 Elsevier GmbH. All rights reserved.

  15. Characterization of ciliate diversity in bromeliad tank waters from the Brazilian Atlantic Forest.

    Science.gov (United States)

    Simão, Taiz L L; Borges, Adriana Giongo; Gano, Kelsey A; Davis-Richardson, Austin G; Brown, Christopher T; Fagen, Jennie R; Triplett, Eric W; Dias, Raquel; Mondin, Claudio A; da Silva, Renata M; Eizirik, Eduardo; Utz, Laura R P

    2017-10-01

    Bromeliads are a diverse group of plants that includes many species whose individuals are capable of retaining water, forming habitats called phytotelmata. These habitats harbor a diversity of organisms including prokaryotes, unicellular eukaryotes, metazoans, and fungi. Among single-celled eukaryotic organisms, ciliates are generally the most abundant. In the present study, we used Illumina DNA sequencing to survey the eukaryotic communities, especially ciliates, inhabiting the tanks of the bromeliads Aechmea gamosepala and Vriesea platynema in the Atlantic Forest of southern Brazil. Filtered sequences were clustered into distinct OTUs using a 99% identity threshold, and then assigned to phylum and genus using a BLAST-based approach (implemented in QIIME) and the SILVA reference database. Both bromeliad species harbored very diverse eukaryotic communities, with Arthropoda and Ciliophora showing the highest abundance (as estimated by the number of sequence reads). The ciliate genus Tetrahymena was the most abundant among single-celled organisms, followed by apicomplexan gregarines and the ciliate genus Glaucoma. Another interesting finding was the presence and high abundance of Trypanosoma in these bromeliad tanks, demonstrating their occurrence in this type of environment. The results presented here demonstrate a hidden diversity of eukaryotes in bromeliad tank waters, opening up new avenues for their in-depth characterization. Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.

  16. Ciliate ingestion and digestion: flow cytometric measurements and regrowth of a digestion-resistant campylobacter jejuni

    Science.gov (United States)

    We developed a method to measure ingestion and digestion rates of bacterivorous protists feeding on pathogenic bacteria. We tested this method using the enteric bacteria Campylobacter jejuni and a freshwater colpodid ciliate. Campylobacter and a non-pathogenic bacteria isolated from the environment ...

  17. Modeling the distribution of ciliate protozoa in the reticulo-rumen using linear programming

    NARCIS (Netherlands)

    Hook, S.E.; Dijkstra, J.; Wright, A.G.; McBride, B.W.; France, J.

    2012-01-01

    The flow of ciliate protozoa from the reticulo-rumen is significantly less than expected given the total density of rumen protozoa present. To maintain their numbers in the reticulo-rumen, protozoa can be selectively retained through association with feed particles and the rumen wall. Few

  18. First report of Epibiont ciliates (Ciliophora: Peritrichia living on larvae of Leptophlebiidae (Ephemeroptera from Brazil

    Directory of Open Access Journals (Sweden)

    Daniel Peroni Abrahão

    2017-05-01

    Full Text Available ABSTRACT Epibiosis was understood as a commensalism relation between two or more organisms. However, some studies have shown that epibionts can cause deleterious effects to their host. Here, we reported the first report of epibiosis between protozoa Epibiont ciliates (Ciliophora: Peritrichia living on larvae of Ephemeroptera from Brazil.

  19. The rumen ciliates of greater kudu Trage/aphus strepsiceros (pallas ...

    African Journals Online (AJOL)

    Studies on the alimentary tract of merino sheep in South Africa. VI. The role of infusoria in ruminal digestion with some remarks on ruminal bacteria. Onderstepoor/ J. Vet. Sci. 17: 61 - 88. VAN HOVEN, W. 1975. Rumen ciliates of the tsessebe (Dama/iscus. /unatus /unatus) in South Africa. J. Protozoo/. 22: 457 - 462. R eprod.

  20. Multiple genes of apparent algal origin suggest ciliates may once have been photosynthetic

    OpenAIRE

    Reyes-Prieto, Adrian; Moustafa, Ahmed; Bhattacharya, Debashish

    2008-01-01

    Plantae (sensu Cavalier-Smith 1981) [1] plastids evolved via primary endosymbiosis whereby a heterotrophic protist enslaved a photosynthetic cyanobacterium. This 'primary' plastid spread into other eukaryotes via secondary endosymbiosis. An important but contentious theory in algal evolution is the chromalveolate hypothesis that posits chromists (cryptophytes, haptophytes, and stramenopiles) and alveolates (ciliates, apicomplexans, and dinoflagellates) share a common ancestor that contained a...

  1. Seasonal and vertical distribution of the ciliated protozoa and micrometazoa in Kaštela Bay (central Adriatic)

    Science.gov (United States)

    Bojanić, N.; Šolić, M.; Krstulović, N.; Marasović, I.; Ninčević, Ž.; Vidjak, O.

    2001-07-01

    Seasonal and vertical distribution of tintinnids, non-loricate ciliates and micrometazoa were studied in Kaštela Bay (central Adriatic Sea) throughout 1995. The species composition of tintinnids and copepods were studied as well. This is the first estimation of non-loricate ciliate biomass in the coastal area of the central Adriatic. Non-loricate ciliates were quantitatively the best represented ciliated protozoa, whereas nauplii were the most numerous micrometazoan organisms. Temperature affected the distribution of most micrometazoan components of the zooplankton and that of non-loricate ciliates. The temperature-dependent presence of individual size categories of non-loricate ciliates was also established. Apart from the interaction between microzooplankton groups, the influence of biotic factors, such as phytoplankton, bacteria, non-pigmented nanoflagellates (NNF) and mesozooplankton, was also discussed. The abundance of ciliates was controlled by both food supply (phytoplankton and NNF) and micrometazoan grazing. The results point to very complex trophic relationships within the planktonic community, suggesting that microzooplankton could be an important link between the microbial food web and higher trophic levels.

  2. The ability of rumen ciliates, Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum, to use the murein saccharides.

    Science.gov (United States)

    Belzecki, G; Miltko, R; Kwiatkowska, E; Michalowski, T

    2013-11-01

    Murein polysaccharides may contribute to a considerable part of the dry matter of bacterial cells. Their utilization by protozoa inhabiting the rumen is, however, poorly recognized. The objective of this study was to examine the ability of three species of ciliates, i.e., Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum of digest, and ferment these saccharides. The cultivation experiments showed that the enrichment of growth medium with bacterial cell wall β-glycans increased the ciliate number (p < 0.05). A statistically significant increase (p < 0.01) was followed by a continuous decrease (p < 0.01) in the percentage of individuals containing β-glycans particles after 4- and 24-h incubation of ciliates with this substrate, respectively. The enzymatic experiments confirmed the ability of the examined protozoa to digest murein. E. caudatum exhibited the highest activity (8.2 unit (U)/mg protein per min), and E. maggii, the lowest (3.0 U/mg protein per min). The production rates of volatile fatty acids by starved and fed ciliate species were 0.7 and 1.6 (E. caudatum) pmol/ciliate cell per h, 30.5 and 42.5 (E. maggii) pmol/ciliate cell per h, and 8.3 and 19.2 (D. affine) pmol/ciliate cell per h (p < 0.05).

  3. DNA Analysis of Algal Endosymbionts of Ciliates Reveals the State of Algal Integration and the Surprising Specificity of the Symbiosis.

    Science.gov (United States)

    Hoshina, Ryo; Kusuoka, Yasushi

    2016-04-01

    Many freshwater protists harbor unicellular green algae within their cells, but little is known of their degree of integration and specificity. Using algae-targeted PCR of whole ciliate cells collected at irregular intervals over 15 months from Lake Biwa, Japan, we explored the SSU-ITS rDNA of the endosymbiotic algae and its changes over time, obtaining sequences of algal rDNA fragments from four ciliate species. A high proportion of clonal algae was evident within the ciliate cells. The differences observed in those sequences from the SSU through to the ITS region were less than 1%. The name 'Chlorb' is proposed for these algae, with the implication that they represent a single 'species.' The sequences of the algal DNA fragments were identical for any given host species throughout the collection period, thus we conclude that these four ciliates stably retain their algae over long term. In contrast, algal DNA fragments obtained from Didinium sp. were variable within each sample, which indicates that this ciliate only temporarily holds its algal cells. The ITS1 sequences of Chlorb populations are close (at intraspecific level) to those of algae isolated from ciliates in Austria, which raises the possibility that Chlorb algae are universally shared as symbionts among various ciliates. Copyright © 2016 Elsevier GmbH. All rights reserved.

  4. Bose-Einstein condensates form in heuristics learned by ciliates deciding to signal 'social' commitments.

    Science.gov (United States)

    Clark, Kevin B

    2010-03-01

    Fringe quantum biology theories often adopt the concept of Bose-Einstein condensation when explaining how consciousness, emotion, perception, learning, and reasoning emerge from operations of intact animal nervous systems and other computational media. However, controversial empirical evidence and mathematical formalism concerning decoherence rates of bioprocesses keep these frameworks from satisfactorily accounting for the physical nature of cognitive-like events. This study, inspired by the discovery that preferential attachment rules computed by complex technological networks obey Bose-Einstein statistics, is the first rigorous attempt to examine whether analogues of Bose-Einstein condensation precipitate learned decision making in live biological systems as bioenergetics optimization predicts. By exploiting the ciliate Spirostomum ambiguum's capacity to learn and store behavioral strategies advertising mating availability into heuristics of topologically invariant computational networks, three distinct phases of strategy use were found to map onto statistical distributions described by Bose-Einstein, Fermi-Dirac, and classical Maxwell-Boltzmann behavior. Ciliates that sensitized or habituated signaling patterns to emit brief periods of either deceptive 'harder-to-get' or altruistic 'easier-to-get' serial escape reactions began testing condensed on initially perceived fittest 'courting' solutions. When these ciliates switched from their first strategy choices, Bose-Einstein condensation of strategy use abruptly dissipated into a Maxwell-Boltzmann computational phase no longer dominated by a single fittest strategy. Recursive trial-and-error strategy searches annealed strategy use back into a condensed phase consistent with performance optimization. 'Social' decisions performed by ciliates showing no nonassociative learning were largely governed by Fermi-Dirac statistics, resulting in degenerate distributions of strategy choices. These findings corroborate

  5. Structural Validity of ‘Santa Clara Strength of Religious Faith Questionnaire’ in Greek Sample

    Directory of Open Access Journals (Sweden)

    Maria Dianni

    2014-02-01

    Full Text Available The aim of the present study was to investigate the structural validity and reliability of the Santa Clara Strength of Religious Faith Questionnaire (SCSRFQ instrument in the Greek population. One hundred and three individuals (men n = 45, and women n = 58 participated in this study. Their age ranged from 17 to 86 years. More specifically, the study investigated the fit of both versions of SCSRFQ (10-item and 5-item. The results of the present study revealed that both versions have adequate fit and can be used by Greek researchers as a measure of strength of religious faith in the Greek population.

  6. Granivorous birds and sorghum crop in the province of Villa Clara,Cuba

    Directory of Open Access Journals (Sweden)

    Orlando Miguel Saucedo Castillo

    2017-07-01

    Full Text Available In order to reduce the damages granivorous birds cause to sorghum (Sorghum bicolor L. Moench in the province of Villa Clara, Cuba, research based on the determination of the major endemic, migratory birds and their relationship with the distribution were made space of historical meteorological variables in the province in the seasonal behavior of birds in different climatic regions. Population to sorghum producers grouped in different forms surveys were conducted, which yielded a large database, such as the determination of the main grain-eating birds percentage damage incurred, varieties, grain color, growth stage and other indicators. Nine main species affecting sorghum grain-eating birds in our province were recorded; Passer domesticus, Lonchura malacca, Lonchura punctulata, Dives atroviolaceus, Passerina cyanea, Zonotrichia leucophrys, Columbina passerine, Zenaida macroura y Zenaida asiatica. The spatial distribution of meteorological variables and their relation to the seasonal behavior of birds in different climatic regions of the province was determined, based on record four preferential habitat areas. The results allowed us to provide companies and different forms of production in Villa Clara, the possibility of a varietal structure planting of sorghum on the basis of different preferential areas granivorous birds, together with the morphological and physiological characteristics of different genotypes introduced in agricultural production of the province and nationally.

  7. Functional diversity of benthic ciliate communities in response to environmental gradients in a wetland of Yangtze Estuary, China.

    Science.gov (United States)

    Xu, Yuan; Fan, Xinpeng; Warren, Alan; Zhang, Liquan; Xu, Henglong

    2018-02-01

    Researches on the functional diversity of benthic ecosystems have mainly focused on macrofauna, and studies on functional structure of ciliate communities have been based only on trophic- or size-groups. Current research was carried out on the changing patterns of classical and functional diversity of benthic ciliates in response to environmental gradients at three sites in a wetland in Yangtze Estuary. The results showed that changes of environmental factors (e.g. salinity, sediment grain size and hydrodynamic conditions) in the Yangtze Estuary induce variability in species composition and functional trait distribution. Furthermore, increased species richness and diversity did not lead to significant changes in functional diversity due to functional redundancy. However, salt water intrusion of Yangtze Estuary during the dry season could cause reduced functional diversity of ciliate communities. Current study provides the first insight into the functional diversity of ciliate communities in response to environmental gradients. Copyright © 2018 Elsevier Ltd. All rights reserved.

  8. INCIDENCIA DE CARDIOPATÍAS DURANTE EL EMBARAZO EN LA PROVINCIA DE VILLA CLARA / Incidence of heart disease during pregnancy in Villa Clara province

    Directory of Open Access Journals (Sweden)

    Marilín C. Luna Alonso

    2012-07-01

    Full Text Available ResumenIntroducción y objetivos: Las enfermedades cardiovasculares constituyen la cuarta causa de muerte durante el período gravídico-puerperal, y un indicador de morbilidad y mortalidad para el producto de la concepción. El objetivo de esta investigación fue determinar el comportamiento de la cardiopatía en la embarazada, su atención médica y evolución durante y al final del embarazo. Método: Se realizó un estudio descriptivo, prospectivo, donde se analizaron 283 gestantes con diagnóstico de cardiopatía, atendidas en el Hospital Universitario Gineco-Obstétrico "Mariana Grajales" de Santa Clara, Villa Clara, en el período 2008-2010. La información se obtuvo mediante la revisión de las historias clínicas individuales, interrogatorio, examen físico completo, así como pruebas complementarias para establecer o confirmar el diagnóstico. Resultados: Se obtuvo una incidencia de 17,2 cardiopatías por cada mil gestantes y en el 59,7 % de ellas, la cardiopatía era adquirida; el prolapso valvular mitral fue la más frecuente (45 %. De las congénitas, el 36,8 % se trataba de una comunicación interauricular. Se utilizó antibioprofilaxis en el 96,8 % de las embarazadas y en el 96,1 % de ellas se mantuvo un seguimiento médico. El 92,6 % tenía clase funcional I y 4,6 % presentó complicación por su enfermedad. El 77,4 % parió entre las 37 y 42 semanas, y el 82,7 % tuvo parto eutócico. Conclusiones: Las gestantes cardiópatas fueron más frecuentes en edades entre 20 y 29 años, con predominio de las que no habían parido, captación entre las 13 y 24 semanas, y cardiopatías adquiridas. La atención médica que prevaleció fue la antibioprofilaxis y el seguimiento médico con evolución final del embarazo satisfactoria. / AbstractIntroduction and Objectives: Cardiovascular diseases are the fourth leading cause of death during the gravid-puerperal period, and an indicator of morbidity and mortality for the product of conception

  9. Analysis of the radioactivity in the 'Elguea' spa, Villa Clara, Cuba; Analisis de la radiactividad en el balneario 'Elguea' , Villa Clara, Cuba

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez G, I.M.; Zerquera, J.T.; Carrazana G, J.; Capote F, E.; Rodriguez C, G. [CPHR, La Habana (Cuba)]. e-mail: isis@cphr.edu.cu

    2006-07-01

    The 'Elguea' spa, located in the north coast of the county of Villa Clara, Cuba, is considered one of those more important spas of Latin America. The waters of this spa are rich in salts of chlorine, sodium, bromine, sulfides and its contain radon. As a result of measurements of environmental gamma dose rates carried out in the area of the spa, its were considered levels of dose between 0.13 and 2.3 mSv. The obtained results motivated that it was carried out a characterization, from the radiological point of view, of the spa. This characterization is of supreme importance to be able to evaluate the impact that it has and the discharges of its waters on the environment, as well as, those dose that the population potentially affected can receive by these discharges. With this end its were carried out quantitative determinations of those existent natural more significant radionuclides in different environmental objects coming from the area of the spa and its surroundings, as well as one characterization gamma 'in situ' of the same one. The obtained results are present in this work. (Author)

  10. Agroproductive educational programs, such as agritourism model for rural development in the canton Santa Clara, Pastaza-Ecuador

    Directory of Open Access Journals (Sweden)

    Karina Carrera Sánchez

    2014-10-01

    Full Text Available The opportunity to connect agriculture with tourism is a new approach as diversify the rural economy, with the vision, this research offers some conceptualizations of agrotourism and the main results of the present situation that allows learning programs potentiate of Centro de Investigación, Posgrado y Conservación de la Biodiversidad Amazónica CIPCA as possible model tourism and agrotourism activity prospects for the development of Santa Clara canton, province of Pastaza, located at km 44 Puyo-Tena. These results are obtained by surveying the producers of Santa Clara and interviews with program productive teaching coordinators and practitioners of center. Methodology we used chi-square test for verification of hypothesis. Eventually show the conclusions that contribute to farm planning for integrated endogenous rural development in Santa Clara.

  11. Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica.

    Science.gov (United States)

    Xu, Yuan; Vick-Majors, Trista; Morgan-Kiss, Rachael; Priscu, John C; Amaral-Zettler, Linda

    2014-10-01

    We report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and March 2008). We tested hypotheses on the relationship between species richness and environmental conditions including environmental extremes, nutrient status, and day length. On the basis of the unique environment that exists in these high-latitude lakes, we expected that novel taxa would be present. Alpha diversity analyses showed that extreme conditions-that is, high salinity, low oxygen, and extreme changes in day length-did not impact ciliate richness; however, ciliate richness was 30% higher in samples with higher dissolved organic matter. Beta diversity analyses revealed that ciliate communities clustered by dissolved oxygen, depth, and salinity, but not by season (i.e., day length). The permutational analysis of variance test indicated that depth, dissolved oxygen, and salinity had significant influences on the ciliate community for the abundance matrices of resampled data, while lake and season were not significant. This result suggests that the vertical trends in dissolved oxygen concentration and salinity may play a critical role in structuring ciliate communities. A PCR-based strategy capitalizing on divergent eukaryotic V9 hypervariable region ribosomal RNA gene targets unveiled two new genera in these lakes. A novel taxon belonging to an unknown class most closely related to Cryptocaryon irritans was also inferred from separate gene phylogenies. © 2014 Marine Biological Laboratory.

  12. Beyond the "Code": A Guide to the Description and Documentation of Biodiversity in Ciliated Protists (Alveolata, Ciliophora).

    Science.gov (United States)

    Warren, Alan; Patterson, David J; Dunthorn, Micah; Clamp, John C; Achilles-Day, Undine E M; Aescht, Erna; Al-Farraj, Saleh A; Al-Quraishy, Saleh; Al-Rasheid, Khaled; Carr, Martin; Day, John G; Dellinger, Marc; El-Serehy, Hamed A; Fan, Yangbo; Gao, Feng; Gao, Shan; Gong, Jun; Gupta, Renu; Hu, Xiaozhong; Kamra, Komal; Langlois, Gaytha; Lin, Xiaofeng; Lipscomb, Diana; Lobban, Christopher S; Luporini, Pierangelo; Lynn, Denis H; Ma, Honggang; Macek, Miroslav; Mackenzie-Dodds, Jacqueline; Makhija, Seema; Mansergh, Robert I; Martín-Cereceda, Mercedes; McMiller, Nettie; Montagnes, David J S; Nikolaeva, Svetlana; Ong'ondo, Geoffrey Odhiambo; Pérez-Uz, Blanca; Purushothaman, Jasmine; Quintela-Alonso, Pablo; Rotterová, Johana; Santoferrara, Luciana; Shao, Chen; Shen, Zhuo; Shi, Xinlu; Song, Weibo; Stoeck, Thorsten; La Terza, Antonietta; Vallesi, Adriana; Wang, Mei; Weisse, Thomas; Wiackowski, Krzysztof; Wu, Lei; Xu, Kuidong; Yi, Zhenzhen; Zufall, Rebecca; Agatha, Sabine

    2017-07-01

    Recent advances in molecular technology have revolutionized research on all aspects of the biology of organisms, including ciliates, and created unprecedented opportunities for pursuing a more integrative approach to investigations of biodiversity. However, this goal is complicated by large gaps and inconsistencies that still exist in the foundation of basic information about biodiversity of ciliates. The present paper reviews issues relating to the taxonomy of ciliates and presents specific recommendations for best practice in the observation and documentation of their biodiversity. This effort stems from a workshop that explored ways to implement six Grand Challenges proposed by the International Research Coordination Network for Biodiversity of Ciliates (IRCN-BC). As part of its commitment to strengthening the knowledge base that supports research on biodiversity of ciliates, the IRCN-BC proposes to populate The Ciliate Guide, an online database, with biodiversity-related data and metadata to create a resource that will facilitate accurate taxonomic identifications and promote sharing of data. © 2017 The Authors Journal of Eukaryotic Microbiology published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists.

  13. Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment

    Directory of Open Access Journals (Sweden)

    Takenaka Akio

    2006-02-01

    Full Text Available Abstract Background The horizontal transfer of expressed genes from Bacteria into Ciliates which live in close contact with each other in the rumen (the foregut of ruminants was studied using ciliate Expressed Sequence Tags (ESTs. More than 4000 ESTs were sequenced from representatives of the two major groups of rumen Cilates: the order Entodiniomorphida (Entodinium simplex, Entodinium caudatum, Eudiplodinium maggii, Metadinium medium, Diploplastron affine, Polyplastron multivesiculatum and Epidinium ecaudatum and the order Vestibuliferida, previously called Holotricha (Isotricha prostoma, Isotricha intestinalis and Dasytricha ruminantium. Results A comparison of the sequences with the completely sequenced genomes of Eukaryotes and Prokaryotes, followed by large-scale construction and analysis of phylogenies, identified 148 ciliate genes that specifically cluster with genes from the Bacteria and Archaea. The phylogenetic clustering with bacterial genes, coupled with the absence of close relatives of these genes in the Ciliate Tetrahymena thermophila, indicates that they have been acquired via Horizontal Gene Transfer (HGT after the colonization of the gut by the rumen Ciliates. Conclusion Among the HGT candidates, we found an over-representation (>75% of genes involved in metabolism, specifically in the catabolism of complex carbohydrates, a rich food source in the rumen. We propose that the acquisition of these genes has greatly facilitated the Ciliates' colonization of the rumen providing evidence for the role of HGT in the adaptation to new niches.

  14. The Use of Ciliates (Ciliophora for Bioassay of the Toxicity of Insecticides

    Directory of Open Access Journals (Sweden)

    Mamedova V. F.

    2016-10-01

    Full Text Available Field and laboratory experiments have been carried out to determine the degree of toxicity in the soil community of ciliates of two insecticides: “Confidor Extra” and “Decis Profi” at concentrations of 80 mg/l, 50mg/l and 25mg/l. Laboratory experiments were performed with the soil ciliates species Colpoda cucullus (Müller, 1773. Changes in phagocytosis and osmoregulation were used as indicators of the survival and physiological parameters. In addition to the laboratory experiments, we carried out field experiments on experimental plots (50 × 50 cm of the virgin soil. Field experiments were carried out in 5 replications. The results showed that the modification of physiological parameters is thinner exponent toxicity than survival.

  15. Necessity Is the Mother of Invention: Ciliates, Transposons, and Transgenerational Inheritance.

    Science.gov (United States)

    Allen, Sarah E; Nowacki, Mariusz

    2017-03-01

    Ciliates are a fascinating model system for the study of the interaction between eukaryotic germlines and somatic lines, especially with regard to the invasion and defence against transposable elements. They separate their germline and somatic line into two nuclei within the same cell, and they silence transposons and repetitive elements by way of deleting them from their somatic genome. This large-scale deletion event uses a series of intricate sequence targeting pathways involving small RNAs and transposases, part of which consists of a transnuclear comparison between maternal soma and daughter germline. We present recent progress in this dynamic field, and argue that these DNA targeting pathways provide an optimal system for the transgenerational inheritance of acquired traits. Ciliates thus also demonstrate the evolutionary value of transposable elements, both as sources of sequence diversity and also as drivers of adaptive evolution by necessitating defensive systems. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Identification and cloning of first cadmium metallothionein like gene from locally isolated ciliate, Paramecium sp.

    Science.gov (United States)

    Shuja, Rukhsana Nighat; Shakoori, Abdul Rauf

    2009-03-01

    First cadmium metallothionein like gene PMCd1 of a ciliate, Paramecium sp., isolated from industrial wastewater has been cloned and sequenced. PMCd1 is an intronless gene, encoding 612 nucleotides, with TAA coding for glutamine. The coding region of PMCd1 comprises 203 amino acids, including 37 cysteine residues with a conserved structural pattern in the form of recurring structural motifs, arranged in 17 x-cys-x-y-cys-x, 1 x-cys-cys-x and x-cys-x contexts. Both, the deduced amino acids and nucleotide sequence differ, not only from other animal metallothioneins (MTs), but also from the previously characterized Tetrahymena Cu and Cd-MTs. The translated protein of PMCd1 contains conserved cysteine residues, peculiar characteristic of stress inducible metallothionein genes of ciliates and other groups of organisms.

  17. [Fibrillar actin in the nuclear apparatus of the ciliate Paramecium caudatum].

    Science.gov (United States)

    Benken, K A; Sabaneeva, E V

    2011-01-01

    Due to their nuclear dualism, ciliates provide a good model for studying the role of actin in spatial organization and transcription activity of the nucleus. The actin in the nuclear apparatus of the ciliate Paramecium caudatum was studied using fluorescently labeled phalloiodin and indirect immunocytochemistry. Fibrillar actin was demonstrated in both of the nuclei. Actin was revealed in the chromatin areas, and was often associated with the periphery of the amplified nucleoli in the macronucleus. Redistribution of actin was observed depending on different physiological state of the cells. Stable infection of the macronulear with the intranuclear endobionts Holospora obtuse led to the loss of nuclear actin accompanied by significant nuclear fragility and redistribution of the phosphorylated form of the actin-binding protein cofilin. Spherical bodies resembling karyosphere were found in the macronuclear anlagen.

  18. CT findings of ciliated hepatic foregut cyst mimicking metastasis: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Kwak, Hyo Sung; Lee, Jeong Min; Kim, In Hwan [Chonbuk National University Hospital, Chonju (Korea, Republic of); Moon, Woo Sung [Medical School, Chonbuk National University, Chonju (Korea, Republic of)

    2000-07-01

    Ciliated hepatic foregut cyst (CHFC) is a rare benign lesion consisting of ciliated pseudostratified columnar epithelium, loose subepithelium, loose subepithelial connective tissue, a smooth muscle layer, and an outer fibrous capsule. we encountered a patient with retroperitoneal and posterior mediastinal neurilemmoma and additional CHFC mimicking metastasis. Abdominal CT examination demonstrated that the posterior mediastinal and retroperitoneal lesions were lobulated, well-defined tumors with aneural foraminal extension that were pathologically confirmed as neurilemmomas. Unenhanced CT indicated that the additional lesion was a slightly hypodense mass relative to surrounding parenchyma at the medial segment of the left lobe of the liver, and after the IV administration of contrast material, the lesion did not show enhancement. (author)

  19. Ciliates rapidly enhance the frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles

    OpenAIRE

    Matsuo, Junji; Oguri, Satoshi; Nakamura, Shinji; Hanawa, Tomoko; Fukumoto, Tatsuya; Hayashi, Yasuhiro; Kawaguchi, Kouhei; Mizutani, Yoshihiko; Yao, Takashi; Akizawa, Kouzi; Suzuki, Haruki; Simizu, Chikara; MATSUNO, Kazuhiko; Kamiya, Shigeru; Yamaguchi, Hiroyuki

    2010-01-01

    The mechanism underlying bacterial conjugation through protozoa was investigated. Kanamycin-resistant Escherichia coli SM10λ+ carrying pRT733 with TnphoA was used as donor bacteria and introduced by conjugation into ciprofloxacin-resistant E. coli clinical isolate recipient bacteria. Equal amounts of donor and recipient bacteria were mixed together in the presence or absence of protozoa (ciliates, free-living amoebae, myxamoebae) in Page's amoeba saline for 24 h. Transconjugants were selected...

  20. Visualization of ex vivo human ciliated epithelium and induced flow using optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Ling, Yuye; Gamm, Uta A.; Yao, Xinwen; Arteaga-Solis, Emilio; Emala, Charles W.; Choma, Michael A.; Hendon, Christine P.

    2017-04-01

    The ciliated epithelium is important to the human respiratory system because it clears mucus that contains harmful microorganisms and particulate matter. We report the ex vivo visualization of human trachea/bronchi ciliated epithelium and induced flow characterized by using spectral-domain optical coherence tomography (SD-OCT). A total number of 17 samples from 7 patients were imaged. Samples were obtained from Columbia University Department of Anesthesiology's tissue bank. After excision, the samples were placed in Gibco Medium 199 solution with oxygen at 4°C until imaging. The samples were maintained at 36.7°C throughout the experiment. The imaging protocol included obtaining 3D volumes and 200 consecutive B-scans parallel to the head-to-feet direction (superior-inferior axis) of the airway, using Thorlabs Telesto system at 1300 nm at 28 kHz A-line rate and a custom built high resolution SDOCT system at 800nm at 32 kHz A-line rate. After imaging, samples were processed with H and E histology. Speckle variance of the time resolved datasets demonstrate significant contrast at the ciliated epithelium sites. Flow images were also obtained after injecting 10μm polyester beads into the solution, which shows beads traveling trajectories near the ciliated epithelium areas. In contrary, flow images taken in the orthogonal plane show no beads traveling trajectories. This observation is in line with our expectation that cilia drive flow predominantly along the superior-inferior axis. We also observed the protective function of the mucus, shielding the epithelium from the invasion of foreign objects such as microspheres. Further studies will be focused on the cilia's physiological response to environmental changes such as drug administration and physical injury.

  1. Ciliates learn to diagnose and correct classical error syndromes in mating strategies

    Directory of Open Access Journals (Sweden)

    Kevin Bradley Clark

    2013-08-01

    Full Text Available Preconjugal ciliates learn classical repetition error-correction codes to safeguard mating messages and replies from corruption by rivals and local ambient noise. Because individual cells behave as memory channels with Szilárd engine attributes, these coding schemes also might be used to limit, diagnose, and correct mating-signal errors due to noisy intracellular information processing. The present study, therefore, assessed whether heterotrich ciliates effect fault-tolerant signal planning and execution by modifying engine performance, and consequently entropy content of codes, during mock cell-cell communication. Socially meaningful serial vibrations emitted from an ambiguous artificial source initiated ciliate behavioral signaling performances known to advertise mating fitness with varying courtship strategies. Microbes, employing calcium-dependent Hebbian-like decision making, learned to diagnose then correct error syndromes by recursively matching Boltzmann entropies between signal planning and execution stages via power or refrigeration cycles. All eight serial contraction and reversal strategies incurred errors in entropy magnitude by the execution stage of processing. Absolute errors, however, subtended expected threshold values for single bit-flip errors in three-bit replies, indicating coding schemes protected information content throughout signal production. Ciliate preparedness for vibrations selectively and significantly affected the magnitude and valence of Szilárd engine performance during modal and nonmodal strategy corrective cycles. But entropy fidelity for all replies mainly improved across learning trials as refinements in engine efficiency. Fidelity neared maximum levels for only modal signals coded in resilient three-bit repetition error-correction sequences. Together, these findings demonstrate microbes can elevate survival/reproductive success by learning to implement classical fault-tolerant information processing in

  2. Water resources development in Santa Clara Valley, California: insights into the human-hydrologic relationship

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, Jesse L. [Univ. of California, Berkeley, CA (United States)

    2000-06-01

    Groundwater irrigation is critical to food production and, in turn, to humankind's relationship with its environment. The development of groundwater in Santa Clara Valley, California during the early twentieth century is instructive because (1) responses to unsustainable resource use were largely successful; (2) the proposals for the physical management of the water, although not entirely novel, incorporated new approaches which reveal an evolving relationship between humans and the hydrologic cycle; and (3) the valley serves as a natural laboratory where natural (groundwater basin, surface watershed) and human (county, water district) boundaries generally coincide. Here, I investigate how water resources development and management in Santa Clara Valley was influenced by, and reflective of, a broad understanding of water as a natural resource, including scientific and technological innovations, new management approaches, and changing perceptions of the hydrologic cycle. Market demands and technological advances engendered reliance on groundwater. This, coupled with a series of dry years and laissez faire government policies, led to overdraft. Faith in centralized management and objective engineering offered a solution to concerns over resource depletion, and a group dominated by orchardists soon organized, fought for a water conservation district, and funded an investigation to halt the decline of well levels. Engineer Fred Tibbetts authored an elaborate water salvage and recharge plan that optimized the local water resources by integrating multiple components of the hydrologic cycle. Informed by government investigations, groundwater development in Southern California, and local water law cases, it recognized the limited surface storage possibilities, the spatial and temporal variability, the relatively closed local hydrology, the interconnection of surface and subsurface waters, and the value of the groundwater basin for its storage, transportation, and

  3. Unusual features of non-dividing somatic macronuclei in the ciliate class Karyorelictea.

    Science.gov (United States)

    Yan, Ying; Rogers, Anna J; Gao, Feng; Katz, Laura A

    2017-10-01

    Genome structure and nuclear organization have been intensely studied in model ciliates such as Tetrahymena and Paramecium, yet few studies have focused on nuclear features of other ciliate clades including the class Karyorelictea. In most ciliates, both the somatic macronuclei and germline micronuclei divide during cell division and macronuclear development only occurs after conjugation. However, the macronuclei of Karyorelictea are non-dividing (i.e. division minus (Div-)) and develop anew from micronuclei during each asexual division. As macronuclei age within Karyorelictea, they undergo changes in morphology and DNA content until they are eventually degraded and replaced by newly developed macronuclei. No less than two macronuclei and one micronucleus are present in karyorelictid species, which suggests that a mature macronucleus 1) might be needed to sustain the cell while a new macronucleus is developing and 2) likely plays a role in guiding the development of the new macronucleus. Here we use a phylogenetic framework to compile information on the morphology and development of nuclei in Karyorelictea, largely relying on the work of Dr. Igor Raikov (1932-1998). We synthesize data to speculate on the functional implications of key features of Karyorelictea including the presence of at least two macronuclei in each cell and the inability for macronuclei to divide. Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.

  4. Assessing phagotrophy in the mixotrophic ciliate Paramecium bursaria using GFP-expressing yeast cells.

    Science.gov (United States)

    Miura, Takashi; Moriya, Hisao; Iwai, Sosuke

    2017-07-03

    We used cells of the yeast Saccharomyces cerevisiae expressing green fluorescent protein (GFP) as fluorescently labelled prey to assess the phagocytic activities of the mixotrophic ciliate Paramecium bursaria, which harbours symbiotic Chlorella-like algae. Because of different fluorescence spectra of GFP and algal chlorophyll, ingested GFP-expressing yeast cells can be distinguished from endosymbiotic algal cells and directly counted in individual P. bursaria cells using fluorescence microscopy. By using GFP-expressing yeast cells, we found that P. bursaria altered ingestion activities under different physiological conditions, such as different growth phases or the presence/absence of endosymbionts. Use of GFP-expressing yeast cells allowed us to estimate the digestion rates of live prey of the ciliate. In contrast to the ingestion activities, the digestion rate within food vacuoles was not affected by the presence of endosymbionts, consistent with previous findings that food and perialgal vacuoles are spatially and functionally separated in P. bursaria. Thus, GFP-expressing yeast may provide a valuable tool to assess both ingestion and digestion activities of ciliates that feed on eukaryotic organisms. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  5. Ciliate communities and hidden biodiversity in freshwater biotopes of the Pistoia province (Tuscany, Italy).

    Science.gov (United States)

    Rossi, Alessia; Boscaro, Vittorio; Carducci, Daniela; Serra, Valentina; Modeo, Letizia; Verni, Franco; Fokin, Sergei I; Petroni, Giulio

    2016-04-01

    Ciliates are essential components of aquatic environments, playing a pivotal role in microbial loops. Thus, the composition and dynamics of ciliate communities have been subjected to intense studying. Morphological methods have been traditionally employed, until the development of next-generation sequencing recently allowed to explore the topic with exclusively molecular techniques. However, the results of the two approaches are hardly comparable, and the pictures they offer can be quite different. This may be due, among other reasons, to two factors: (1) morphological descriptions may miss a large portion of "hidden biodiversity" (including rare species and resistance forms) that is detected instead by molecular methods; (2) identification errors may arise due to difficulties in recognizing microbial taxa without in-depth analyses. In this survey of freshwater systems of the Pistoia province (Tuscany, Italy) we address both issues, trying to quantify the hidden diversity through prolonged observations of differentially treated sample aliquots, combining morphological identification with Sanger sequencing. We provide the first insights into the ciliate fauna of this area presenting results that are suitable for future comparisons thanks to their multidisciplinary origin, and supply the first molecular data on well-known taxa such as Linostomella and Disematostoma. Copyright © 2015 Elsevier GmbH. All rights reserved.

  6. The marine mixotroph, Mesodinium rubrum is far more than a greenhouse ciliate

    Science.gov (United States)

    Yih, W.; Myung, G.; Kim, H. S.; Yoo, Y. D.; Rho, J. R.

    2016-02-01

    Permanent symbiosis between the mixotrophic ciliate Mesodinium rubrum and the cryptomonad symbionts has long been assumed since 1908, when Hans Lohmann firstly described the reddish-brown globules inside M. rubrum ("Halteria rubra") cells as symbiotic algae ("Erythromonas haltericola"). Thus, M. rubrum was envisioned as a host greenhouse where numerous cryptomonad symbionts could be farmed. During last two decades, however, information on the species interaction among marine protists including M. rubrum was so impressively accumulated that the more real picture of the `symbiotic relationship' could be revealed. In addition to the obligate replacement of the selected organelles from a `symbiont', multiple donor strains for the klepto-organelles of M. rubrum was also explored. Hence, experimentally designed organelle trades for M. rubrum is not impossible today. This unique mixotrophic ciliate must be a pivotal member of marine plankton ecosystem with its superior klepto-organelles, motility, growth rate, and linkablilty to higher trophic levels. M. rubrum can link marine heterotrophic bacteria and cyanobacteria to its own predators which in turn could be consumed by other carnivores. Supported by the klepto-organelles and vitamins from prey cryptomonads as well as N(from cyanobacteria) and P(from heterotrophic bacteria) nutrients, M. rubrum thrives at diverse marine environments. Bacterivory by the protistan members of `Mesodinium food chain' may need to be further studied before we can better understand the superiority of the unique ciliate species in the sea.

  7. Respiration, growth and grazing rates of three ciliate species in hypoxic conditions.

    Science.gov (United States)

    Rocke, Emma; Liu, Hongbin

    2014-08-30

    Marine hypoxic episodes are affecting both marine and freshwater bodies all over the world. Yet, limited data exists with regard to the effects of decreasing oxygen on protist metabolism. Three ciliate species were therefore isolated from Hong Kong coastal waters. Controlled hypoxic conditions were simulated in the lab environment, during which time growth, respiration and grazing rates were measured. Euplotes sp. and a Oxytrichidae-like ciliate showed decreased growth and respiration below 2.5 mg O2 L(-1), however Uronema marinum kept steady growth and respiration until below 1.5 mg O2 L(-1). Euplotes sp. and the Oxytrichidae-like ciliate had the highest ingestion rate, which dropped significantly below 3.0 mg O2 L(-1). U.marinum grazing rates were affected at and below 1.5 mg O2 L(-1), correlating with their drop in growth and respiration at this lower concentration. This study illustrates the slowing metabolism of key grazing protists, as well as species-specific tolerance in response to hypoxia. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle.

    Science.gov (United States)

    Tay, Hwee Goon; Schulze, Sabrina K; Compagnon, Julien; Foley, Fiona C; Heisenberg, Carl-Philipp; Yost, H Joseph; Abdelilah-Seyfried, Salim; Amack, Jeffrey D

    2013-04-01

    Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer's vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish.

  9. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle

    Science.gov (United States)

    Tay, Hwee Goon; Schulze, Sabrina K.; Compagnon, Julien; Foley, Fiona C.; Heisenberg, Carl-Philipp; Yost, H. Joseph; Abdelilah-Seyfried, Salim; Amack, Jeffrey D.

    2013-01-01

    Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer’s vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer’s vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer’s vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish. PMID:23482490

  10. Protozoa ciliates community structure in urban streams and their environmental use as indicators

    Directory of Open Access Journals (Sweden)

    C. Debastiani

    Full Text Available Abstract The objective of this work was to investigate the patterns of diversity and abundance of ciliates protozoa community in three tropical urban streams and verify which species can be considered bioindicators of water quality. In each stream, three regions (headwater, middle and mouth were sampled in two years, in the rainy and dry seasons. The abiotic factors (dissolved oxygen, pH, temperature, turbidity, width, depth, flow and conductivity and biotic factors (total coliforms, bacterioplankton, chlorophyll and flagellates were analyzed using appropriate methods and ciliates were identified and counted through specialized literature. We identified 84 species distributed into 24 orders. Peniculida was the most representative order followed by Prorodontida. The RDA scores showed a spatial difference between streams. On the other hand, regarding the temporal variation, there was no separation of the sampled data. The Indval analysis showed ten species indicators, such as Coleps hirtus, Euplotes moebiusi and Tintinidium pusillum. The Cluster analysis per stream versus period showed a greater distinction of the streams in the dry season. On the other hand, a low similarity was observed between sections within the same stream. In summary, the results indicated that the ciliates can be used as important tools as bioindicators in lotic environments affected by different degrees of pollution, due to the fact that they have short life cycle, allowing the detection of impacts on a small time scale, as well as by having direct answers to environmental changes and high sensitivity to contaminants.

  11. Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles

    Directory of Open Access Journals (Sweden)

    George B Mcmanus

    2012-09-01

    Full Text Available Simultaneous use of both heterotrophic and autotrophic metabolism (mixotrophy is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and southwest Atlantic Ocean show similar numerical response parameters (maximum growth rate, food concentration at which growth is half its maximum, and threshold food concentration for growth, and some isolates have been maintained in vitro for over three years. This ciliate grows equally well when fed on the green alga Tetraselmis chui (strain PLY429 or the cryptophyte Rhodomonas lens (strain RHODO. It appears to be an obligate mixotroph, requiring both food and light to achieve positive growth, when feeding on either of these algae. However, it has also been grown for several weeks (> 10 generations heterotrophically on the dinoflagellate Prorocentrum minimum (strain EXUV during which it grows better in dark than in light. In this paper, we review the ecology of S. rassoulzadegani, discuss some aspects of its photo- and feeding physiology, and speculate on benefits and costs to the ciliate of chloroplast symbiosis.

  12. Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    Science.gov (United States)

    McManus, George B; Schoener, Donald M; Haberlandt, Katharine

    2012-01-01

    Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and southwest Atlantic Ocean show similar numerical response parameters (maximum growth rate, food concentration at which growth is half its maximum, and threshold food concentration for growth), and some isolates have been maintained in vitro for over 3 years. This ciliate grows equally well when fed on the green alga Tetraselmis chui (strain PLY429) or the cryptophyte Rhodomonas lens (strain RHODO). It appears to be an obligate mixotroph, requiring both food and light to achieve positive growth, when feeding on either of these algae. However, it has also been grown for several weeks (>10 generations) heterotrophically on the dinoflagellate Prorocentrum minimum (strain EXUV) during which it grows better in dark than in light. In this paper, we review the ecology of S. rassoulzadegani, discuss some aspects of its photo- and feeding physiology, and speculate on benefits and costs to the ciliate of chloroplast symbiosis.

  13. A case of foregut gastric duplication cyst with pseudostratified columnar ciliated epithelium.

    Science.gov (United States)

    Hosomura, Naohiro; Kono, Hiroshi; Kawaida, Hiromichi; Amemiya, Hidetake; Itakura, Jun; Fujii, Hideki

    2012-02-01

    Gastrointestinal duplication is a congenital rare disease. Duplication cyst of the stomach with pseudostratified columnar ciliated epithelium is extremely rare. A 44-year-old Japanese woman visited University of Yamanashi Hospital for evaluation of an abnormal tumor detected by abdominal ultrasonography at an annual general health examination. Abdominal computed tomography indicated a subserosal cystic lesion 6 cm in diameter on the posterior wall of the stomach. The cystic lesion was resected through partial resection of the stomach. Histopathology showed that the cyst did not communicate with the gastric lumen, was covered with gastric epithelium and pseudostratified columnar ciliated epithelium with circular muscle layers, and did not contain cartilaginous tissue. Consequently, the patient was diagnosed as having foregut duplication cyst of the stomach. Gastrointestinal duplication can occur in any region of the gastrointestinal tract, but foregut duplication cyst of the stomach is rare. The present case was a subserosal cyst on the greater curvature that did not communicate with the gastric lumen and was covered with gastric epithelium and pseudostratified columnar ciliated epithelium, suggesting a foregut cyst caused by an aberrant respiratory organ.

  14. Unfolding Thermodynamics of Cysteine-Rich Proteins and Molecular Thermal-Adaptation of Marine Ciliates

    Directory of Open Access Journals (Sweden)

    Giorgia Cazzolli

    2013-11-01

    Full Text Available Euplotes nobilii and Euplotes raikovi are phylogenetically closely allied species of marine ciliates, living in polar and temperate waters, respectively. Their evolutional relation and the sharply different temperatures of their natural environments make them ideal organisms to investigate thermal-adaptation. We perform a comparative study of the thermal unfolding of disulfide-rich protein pheromones produced by these ciliates. Recent circular dichroism (CD measurements have shown that the two psychrophilic (E. nobilii and mesophilic (E. raikovi protein families are characterized by very different melting temperatures, despite their close structural homology. The enhanced thermal stability of the E. raikovi pheromones is realized notwithstanding the fact that these proteins form, as a rule, a smaller number of disulfide bonds. We perform Monte Carlo (MC simulations in a structure-based coarse-grained (CG model to show that the higher stability of the E. raikovi pheromones is due to the lower locality of the disulfide bonds, which yields a lower entropy increase in the unfolding process. Our study suggests that the higher stability of the mesophilic E. raikovi phermones is not mainly due to the presence of a strongly hydrophobic core, as it was proposed in the literature. In addition, we argue that the molecular adaptation of these ciliates may have occurred from cold to warm, and not from warm to cold. To provide a testable prediction, we identify a point-mutation of an E. nobilii pheromone that should lead to an unfolding temperature typical of that of E. raikovi pheromones.

  15. Caracterización de las farmacias comunitarias de Santa Clara para la implementación de la Dispensación de Medicamentos Characterization of community pharmacies in Santa Clara city for the implementation of drug dispensing

    National Research Council Canada - National Science Library

    Liset Jaramillo Hernández; Abdel Bermúdez del Sol; Yelien Martín Fadragas; Jorge Luis Morales Martínez; Ydanelly San Gabino Paredes

    2012-01-01

    ...: caracterizar las farmacias comunitarias de Santa Clara para la implementación del Servicio de Dispensación de Medicamentos. Métodos: se realizó una investigación en sistemas y servicios de salud con un enfoque...

  16. Migration, Memory and Hong Kong as a 'Space of Transit' in Clara Law's Autumn Moon

    Directory of Open Access Journals (Sweden)

    Tony Mitchell

    2013-09-01

    Full Text Available Macau-born and Melbourne-based film maker Clara Law and her screenwriter-producer-director husband Eddie Fong have produced a transnational output of films which are beginning to receive critical recognition as major contributions to contemporary cinema. These ‘films of migration’ explore what Gina Marchetti has encapsulated as ‘the Chinese experience of dislocation, relocation, emigration, immigration, cultural hybridity, migrancy, exile, and nomadism—together termed the “Chinese diaspora”’. The self-imposed ‘relocation’ of Law and Fong to Australia in 1994 was the result of increasing frustration with the rampantly commercial imperatives of Hong Kong cinema and its lack of appreciation for the auteur cinema they wanted to pursue.

  17. EFEITO DE ÉSTERES DE SACAROSE NO ARMAZENAMENTO DE TOMATES `SANTA CLARA'

    Directory of Open Access Journals (Sweden)

    Kluge R.A

    1997-01-01

    Full Text Available Tomates cultivar Santa Clara foram colhidos em 10/10/1995 e tratados com Semperfresh (ésteres de sacarose a 0; 0,5; 1,0; 1,5 e 2,0%, imersão (1 minuto. Os frutos foram armazenados por 15 dias a 25oC e 70% UR. Os frutos tratados com Semperfresh apresentaram menor desenvolvimento de coloração e menores perdas de firmeza de polpa do que os não tratados. Não houve efeito sobre a perda de peso e os sólidos solúveis totais. Das concentrações utilizadas, o melhor resultado foi obtido com 2% de Semperfresh.

  18. Startup, testing, and operation of the Santa Clara 2MW direct carbonate fuel cell demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    Skok, A.J.; Leo, A.J. [Fuel Cell Engineering Corp., Danbury, CT (United States); O`Shea, T.P. [Santa Clara Demonstration Project, CA (United States)

    1996-12-31

    The Santa Clara Demonstration Project (SCDP) is a collaboration between several utility organizations, Fuel Cell Engineering Corporation (FCE), and the U.S. Dept. Of Energy aimed at the demonstration of Energy Research Corporation`s (ERC) direct carbonate fuel cell (DFC) technology. ERC has been pursuing the development of the DFC for commercialization near the end of this decade, and this project is an integral part of the ERC commercialization effort. The objective of the Santa Clara Demonstration Project is to provide the first full, commercial scale demonstration of this technology. The approach ERC has taken in the commercialization of the DFC is described in detail elsewhere. An aggressive core technology development program is in place which is focused by ongoing interaction with customers and vendors to optimize the design of the commercial power plant. ERC has selected a 2.85 MW power plant unit for initial market entry. Two ERC subsidiaries are supporting the commercialization effort: the Fuel Cell Manufacturing Corporation (FCMC) and the Fuel Cell Engineering Corporation (FCE). FCMC manufactures carbonate stacks and multi-stack modules, currently from its production facility in Torrington, CT. FCE is responsible for power plant design, integration of all subsystems, sales/marketing, and client services. FCE is serving as the prime contractor for the design, construction, and testing of the SCDP Plant. FCMC has manufactured the multi-stack submodules used in the DC power section of the plant. Fluor Daniel Inc. (FDI) served as the architect-engineer subcontractor for the design and construction of the plant and provided support to the design of the multi-stack submodules. FDI is also assisting the ERC companies in commercial power plant design.

  19. Comportamento do camarão em diferentes substratos nas fases clara e escura do dia

    Directory of Open Access Journals (Sweden)

    Daniele Bezerra dos Santos

    2013-08-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do tipo de substrato sobre a preferência, o enterramento e a atividade natatória de camarões (Litopenaeus vannamei. Juvenis (0,93±0,29 g e adultos machos e fêmeas (10,05±1,18 g foram observados durante 24 horas, por meio do método animal focal, com registros instantâneos. Foram testadas as seguintes granulometrias (tratamentos dos substratos: I, 100% C; II, 50% C + 50% B; III, 50% A + 50% C; IV, 100% B; V, 50% B + 50% A; e VI, 100% A, em que as letras referemse ao diâmetro médio das partículas, A = 0,25-2,0 mm; B = 0,0625-0,25 mm; e C = <0,0625 mm. Foram aplicados seis tratamentos com 18 repetições para cada categoria: camarões juvenis, machos e fêmeas. Cada indivíduo foi exposto por 24 horas aos seis tipos de substrato, simultaneamente, e o seu comportamento foi observado nas fases clara e escura do dia. A natação ocorreu principalmente na fase escura e o enterramento, na fase clara, no substrato IV. Os camarões juvenis e adultos (machos ou fêmeas preferem o substrato composto de areia fina e muito fina (0,0625-0,25 mm, tanto para permanência quanto para enterramento.

  20. Evaluación de la satisfacción del cliente de la Drogueria Villa Clara, 2008-2009 Assessment of the client satisfaction in Villa Clara Drugstore, 2008-2009

    Directory of Open Access Journals (Sweden)

    Geidy Lorezo Monteagudo

    2012-03-01

    Full Text Available Introducción: la Droguería Villa Clara es la encargada de la distribución de medicamentos, reactivos y otros insumos médicos en esta provincia de Cuba. Mantiene relaciones comerciales con gran variedad de clientes, pero los más importantes son los pertenecientes al Sistema de Salud. Objetivo: evaluar la calidad del servicio brindado por esta entidad a los hospitales, policlínicos y farmacias de la provincia durante el 2008 y el 2009. Métodos: se conformó una muestra representativa de estas instituciones en todos los municipios de Villa Clara y se aplicaron encuestas al personal responsable de medicamentos en cada tipo unidad. Se determinó el índice de satisfacción para cada cliente encuestado en particular, así como para policlínicos, hospitales y farmacias en sentido general. Por otra parte, se determinó el índice de satisfacción alcanzado de forma global por la droguería en cada año evaluado. Resultados: el servicio brindado por la Droguería Villa Clara durante el 2008 y el 2009 fue calificado como bueno, con una mejora palpable al obtenerse índices de satisfacción que variaron de 84,2 % en el 2008 a 88,3 % en el 2009. Conclusiones: los clientes del proceso de distribución de medicamentos, perciben la disponibilidad de medicamentos como el problema fundamental de la distribución en la provincia.Introduction: Villa Clara Drugstore is in charge of the distribution of drugs, reagents, and other medical inputs in this Cuban province. It has commercial relations with a wide variety of clients, but the most important ones are those within the health system. Objective: to evaluate the quality of service rendered by this entity to hospitals, polyclinics and pharmacies in the province in 2008 and 2009. Methods: a representative sample from these institutions in all Villa Clara municipalities was selected and the personnel in charge of medications in each unit was surveyed. Satisfaction index for each surveyed client was

  1. Subsurface and petroleum geology of the southwestern Santa Clara Valley ("Silicon Valley"), California

    Science.gov (United States)

    Stanley, Richard G.; Jachens, Robert C.; Lillis, Paul G.; McLaughlin, Robert J.; Kvenvolden, Keith A.; Hostettler, Frances D.; McDougall, Kristin A.; Magoon, Leslie B.

    2002-01-01

    Gravity anomalies, historical records of exploratory oil wells and oil seeps, new organic-geochemical results, and new stratigraphic and structural data indicate the presence of a concealed, oil-bearing sedimentary basin beneath a highly urbanized part of the Santa Clara Valley, Calif. A conspicuous isostatic-gravity low that extends about 35 km from Palo Alto southeastward to near Los Gatos reflects an asymmetric, northwest-trending sedimentary basin comprising low-density strata, principally of Miocene age, that rest on higher-density rocks of Mesozoic and Paleogene(?) age. Both gravity and well data show that the low-density rocks thin gradually to the northeast over a distance of about 10 km. The thickest (approx 4 km thick) accumulation of low-density material occurs along the basin's steep southwestern margin, which may be controlled by buried, northeast-dipping normal faults that were active during the Miocene. Movement along these hypothetical normal faults may been contemporaneous (approx 17–14 Ma) with sedimentation and local dacitic and basaltic volcanism, possibly in response to crustal extension related to passage of the northwestward-migrating Mendocino triple junction. During the Pliocene and Quaternary, the normal faults and Miocene strata were overridden by Mesozoic rocks, including the Franciscan Complex, along northeastward-vergent reverse and thrust faults of the Berrocal, Shannon, and Monte Vista Fault zones. Movement along these fault zones was accompanied by folding and tilting of strata as young as Quaternary and by uplift of the modern Santa Cruz Mountains; the fault zones remain seismically active. We attribute the Pliocene and Quaternary reverse and thrust faulting, folding, and uplift to compression caused by local San Andreas Fault tectonics and regional transpression along the Pacific-North American Plate boundary. Near the southwestern margin of the Santa Clara Valley, as many as 20 exploratory oil wells were drilled between 1891

  2. Macronuclear genome structure of the ciliate Nyctotherus ovalis: Single-gene chromosomes and tiny introns

    Directory of Open Access Journals (Sweden)

    Landweber Laura F

    2008-12-01

    Full Text Available Abstract Background Nyctotherus ovalis is a single-celled eukaryote that has hydrogen-producing mitochondria and lives in the hindgut of cockroaches. Like all members of the ciliate taxon, it has two types of nuclei, a micronucleus and a macronucleus. N. ovalis generates its macronuclear chromosomes by forming polytene chromosomes that subsequently develop into macronuclear chromosomes by DNA elimination and rearrangement. Results We examined the structure of these gene-sized macronuclear chromosomes in N. ovalis. We determined the telomeres, subtelomeric regions, UTRs, coding regions and introns by sequencing a large set of macronuclear DNA sequences (4,242 and cDNAs (5,484 and comparing them with each other. The telomeres consist of repeats CCC(AAAACCCCn, similar to those in spirotrichous ciliates such as Euplotes, Sterkiella (Oxytricha and Stylonychia. Per sequenced chromosome we found evidence for either a single protein-coding gene, a single tRNA, or the complete ribosomal RNAs cluster. Hence the chromosomes appear to encode single transcripts. In the short subtelomeric regions we identified a few overrepresented motifs that could be involved in gene regulation, but there is no consensus polyadenylation site. The introns are short (21–29 nucleotides, and a significant fraction (1/3 of the tiny introns is conserved in the distantly related ciliate Paramecium tetraurelia. As has been observed in P. tetraurelia, the N. ovalis introns tend to contain in-frame stop codons or have a length that is not dividable by three. This pattern causes premature termination of mRNA translation in the event of intron retention, and potentially degradation of unspliced mRNAs by the nonsense-mediated mRNA decay pathway. Conclusion The combination of short leaders, tiny introns and single genes leads to very minimal macronuclear chromosomes. The smallest we identified contained only 150 nucleotides.

  3. The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa.

    Science.gov (United States)

    Biel, Merrill A; Jones, John W; Pedigo, Lisa; Gibbs, Aaron; Loebel, Nicolas

    2012-12-01

    Chronic recurrent sinusitis (CRS) is one of the most common chronic conditions in the United States. There is a significant subpopulation of CRS patients who remain resistant to cure despite rigorous treatment regimens including surgery, allergy therapy, and prolonged antibiotic therapy. Antimicrobial photodynamic therapy (aPDT) is a noninvasive nonantibiotic broad spectrum antimicrobial treatment. Our previous in vitro studies demonstrated that aPDT reduced CRS polymicrobial biofilm and planktonic bacteria and fungi by > 99.9% after a single treatment. Prior to human treatment however, aPDT treatment must be demonstrated to not result in histologic damage to the sinus ciliated respiratory epithelium. The objective of this study was to demonstrate the safety of aPDT treatment on a living human ciliated respiratory mucosal model (EpiAirway). A study of aPDT treatment of EpiAirway was performed. Treatment groups included a nontreatment control, laser light alone, photosensitizer alone, and therapeutic photosensitizer and light combination (aPDT). At completion of treatment, the EpiAirway tissue was fixed in 10% formalin, paraffin-embedded, sectioned, H&E stained and mounted. All samples were blinded and microscopically examined by a human pathologist to assess any effect of aPDT on the tissue, cilia, or mucosal glands. The results were correlated with the treatment parameters. The EpiAirway histologic study demonstrated no histologic alteration of the respiratory cilia or mucosal epithelium in any of the treatment groups. aPDT is a safe treatment for CRS resulting in no histologic alteration of human ciliated respiratory mucosa as is found in the human sinuses. Copyright © 2012 The American Laryngological, Rhinological, and Otological Society, Inc.

  4. Experimental identification and analysis of macronuclear non-coding RNAs from the ciliate Tetrahymena thermophila

    DEFF Research Database (Denmark)

    Andersen, Kasper Langebjerg; Nielsen, Henrik

    2012-01-01

    The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization. Recent work has shown that Tetrahymena has many classes of small RNA molecules...... class was small nucleolar RNAs (snoRNAs), many of which are formed by an unusual maturation pathway. The modifications guided by the snoRNAs were analyzed bioinformatically and experimentally and many Tetrahymena-specific modifications were found, including several in an essential, but not conserved...

  5. Novel Insights into the Genetic Diversity of Balantidium and Balantidium-like Cyst-forming Ciliates

    OpenAIRE

    Kateřina Pomajbíková; Miroslav Oborník; Aleš Horák; Petrželková, Klára J.; Norman Grim, J.; Bruno Levecke; Angelique Todd; Martin Mulama; John Kiyang; David Modrý

    2013-01-01

    Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were compu...

  6. Dealing with the presence of the ciliate Euplotes sp. in cultures of the copepod Acartia tonsa

    DEFF Research Database (Denmark)

    Drillet, Guillaume; Dutz, Jörg

    2014-01-01

    of Acartia tonsa fed with Rhodomonas salina. We found that at a concentration of 2 cells ml-1, Euplotes sp. had no effect on the production and hatching success of eggs but increased/decreased the mortality/quality of non-subitaneous eggs. Euplotes sp. had a good fatty acid profile containing high proportion...... of unsaturated fatty acids, but the amount of fatty acids compared to their carbon weight was very low and, therefore, it was considered as a poor food source. We propose a short-term interruption of food supply to copepod cultures in order to mitigate bloom formation of ciliates. This will force copepods...

  7. Ciliate protozoa in the rumen of Kafue lechwe, Kobus leche kafuensis, in Zambia, with the description of four new species.

    Science.gov (United States)

    Imai, S; Tsutsumi, Y; Yumura, S; Mulenga, A

    1992-01-01

    The composition of the rumen ciliate fauna in 76 Kafue lechwe inhabiting a limited area in Zambia was surveyed and five genera containing 24 species with 16 formae belonging to the family Ophryoscolecidae were identified. Four new species belonging to Diplodiniinae were recognized and described as Diplodinium lochinvarense n. sp., Diplodinium leche n. sp., Diplodinium zambiense n. sp., and Metadinium ossiculi n. sp. In addition, Ostracodinium gracile form fissilaminatum Dogiel, 1932 was found for the second time and described as Metadinium fissilaminatum n. comb. The species composition was fairly unusual. Seven of the species have been found only in African wild antelopes and these species were found more frequently than cosmopolitan species. There was no evidence of isotrichid species. The average density of ciliates per 1 ml of the rumen fluid was 25.7 x 10(4), and the number of ciliate species per head of host was 10.8.

  8. Natural variation in methane emission of sheep fed on a lucerne pellet diet is unrelated to rumen ciliate community type.

    Science.gov (United States)

    Kittelmann, Sandra; Pinares-Patiño, Cesar S; Seedorf, Henning; Kirk, Michelle R; McEwan, John C; Janssen, Peter H

    2016-03-01

    Only limited information is available on the roles of different rumen ciliate community types, first described by Eadie in 1962, in enteric methane (CH4) formation by their ruminant hosts. If the different types were differentially associated with CH4 formation, then ciliate community typing could be used to identify naturally high and low CH4-emitting animals. Here we measured the CH4 yields [g CH4 (kg feed dry matter intake, DMI)(-1)] of 118 sheep fed a standard pelleted lucerne diet at two different times, at least 2 weeks apart. There were significant differences (P sheep selected as high [16.7 ± 1.5 g CH4 (kg DMI)(-1)] and low emitters [13.3 ± 1.5 g CH4 (kg DMI)(-1)]. A rumen sample was collected after each of the two measurements, and ciliate composition was analysed using barcoded 454 Titanium pyrosequencing of 18S rRNA genes. The genera found, in order of mean relative abundance, were Epidinium, Entodinium, Dasytricha, Eudiplodinium, Polyplastron, Isotricha and Anoplodinium-Diplodinium, none of which was significantly correlated with the CH4 emissions ranking associated with the rumen sample. Ciliate communities naturally assembled into four types (A, AB, B and O), characterized by the presence and absence of key genera. There was no difference in CH4 yield between sheep that harboured different ciliate community types, suggesting that these did not underlie the natural variation in CH4 yields. Further research is needed to unravel the nature of interactions between ciliate protozoa and other rumen micro-organisms, which may ultimately lead to contrasting CH4 emission phenotypes.

  9. Elastic stresses and plastic deformations in 'Santa Clara' tomato fruits caused by package dependent compression

    Directory of Open Access Journals (Sweden)

    PEREIRA ADRIANA VARGAS

    2000-01-01

    Full Text Available The objective of this work was to study the fruit compression behavior aiming to develop new tomato packages. Deformations caused by compression forces were observed inside packages and in individual 'Santa Clara' tomato fruit. The forces applied by a transparent acrylic lever to the fruit surface caused pericarp deformation and the flattened area was proportional to the force magnitude. The deformation was associated to the reduction in the gas volume (Vg, caused by expulsion of the air from the loculus cavity and reduction in the intercellular air volume of the pericarp. As ripening advanced, smaller fractions of the Vg reduced by the compressive force were restored after the stress was relieved. The lack of complete Vg restoration was an indication of permanent plastic deformations of the stressed cells. Vg regeneration (elastic recovery was larger in green fruits than in the red ones. The ratio between the applied force and the flattened area (flattening pressure, which depends on cell turgidity, decreased during ripening. Fruit movements associated with its depth in the container were observed during storage in a transparent glass container (495 x 355 x 220 mm. The downward movement of the fruits was larger in the top layers because these movements seem to be driven by a summation of the deformation of many fruits in all layers.

  10. Quantitative estimation of the carbon footprint in the sugar cane culture in Villa Clara

    Directory of Open Access Journals (Sweden)

    Elier Gavilán Martínez

    2017-01-01

    Full Text Available The carbon footprint in the sugar cane sector in Villa Clara province was determined by using as cases study two Agricultural Production Cooperatives (CPA dedicated to sugar cane cropping. For this purpose, the environmental aspects associated with the sugar cane cultivation, from the land preparation for the industry delivery, were quantified. Data matching to each environmental aspect were collected and subjected to triangulation using Participatory-Action Research techniques and tools. CO2 -eq emissions per hectare show significant differences between the study cases, matching the higher value to the “Jesús Menéndez” CPA. The consumption of energy sources, non-organic materials and the industrial gasses, as well as the use of soil, are the environmental aspects with the higher burden on the carbon footprint in both study cases, with percentage values upper than 11 % out of total CO2 -eq emissions. The CO2 -eq average annual emissions in relation to the physical production are significantly higher at the “Jesús Menéndez” CPA, that’s why the crop of one tonne of sugar cane in the CPA implies a higher economic cost compared with the “4 de abril” CPA. It is concluded that the productive systems of both cases studies have a similar performance regarding their contribution to the global warming.

  11. How the Anatomische Gesellschaft excluded unwanted members after 1945-among them Eugen Fischer and Max Clara.

    Science.gov (United States)

    Winkelmann, Andreas

    2017-01-01

    The Anatomische Gesellschaft, an international Germany-based association of anatomists, was closed down in 1945, after the end of the "Third Reich". It was eventually re-founded in 1949, continuing its tradition from its foundation in 1886, based in large part on the membership prior to 1945. Newly available archival material reveals, however, that at least six members were explicitly prevented from re-joining the society. This includes Max Clara, who was accused of plagiarism and, at least implicitly, of basing his career on Nazi party support. It also includes Eugen Fischer, a leading anthropologist of the Nazi period, who was seen to be indirectly responsible for Nazi crimes like forced sterilisation or extermination of "anthropologically defined" groups of people. Therefore, Fischer's honorary membership, which had already been published in the membership directory, was revoked after a heated internal debate. Nevertheless, these exclusions cannot be interpreted as a self-directed "denazification" of the Anatomische Gesellschaft, as political activity in line with the Nazis was not the main criterion for these exclusions. Incidentally, the archival sources also reveal that Wolfgang Bargmann, who had been elected as the first post-war secretary of the Gesellschaft in 1949, resigned from this post after only one year in office because his management of this "Fischer affair" was felt to be too autocratic. Copyright © 2016 Elsevier GmbH. All rights reserved.

  12. The Ecological Footprint of the Agrarian Sector in Villa Clara. A qualitative approximation

    Directory of Open Access Journals (Sweden)

    Mario Reinoso Pérez

    2015-01-01

    Full Text Available This research deals with the environmental impacts and aspects and their relationship to the ecological footprint of the agrarian sector in Villa Clara. In order to achieve this goal, 23 enterprises were used as study cases, which were clustered according to the main land use: (i succession planting (17 and (ii livestock and animal production (6. The information collected was used to develop the environmental aspects and impacts matrixes associated to the productive processes in each enterprise. The environmental aspects with higher relative frequencies (> 0.60 were water and energy consumption, the existence of invasive plants, the soil use, the greenhouse gas emissions, the use of forest resources, the generation of solid wastes and the use of agro-chemicals. Such aspects are causing negative impacts on the environment, and consequently they are increasing the ecological print of the sector in this province. The use of heavy machineries, the generation of liquid and dangerous residues, the continuous grazing and the use of alien species had a significant impact with a relative frequency below 0.55. However, these environmental aspects should be under control. It is concluded that, in all of the studied cases, the environmental aspects had a negative incidence on the environment. And they contribute in many ways to increase the ecological footprint of the agrarian sector in the territory. The implementation of the Environmental Management System, according to the international standards, is recommended.

  13. SUGAR AGROINDUSTRY IMPACT IN LOCAL DEVELOPMENT OF ENCRUCIJADA MUNICIPALITY IN VILLA CLARA PROVINCE

    Directory of Open Access Journals (Sweden)

    Ramón Sánchez Sánchez

    2017-07-01

    Full Text Available The purpose of this investigation is to show the impact in the economic, energy, ecological and sociocultural order that has experienced the sugar agroindustry in the local and territorial development of Encrucijada municipality, in Villa Clara province. The main methods and techniques used have been the surveys, the documentary analysis, the work with experts, agrarian commission’s reports of the municipal assembly, the matrix of weaknesses, strengths, threats, opportunities and the scenarios technique for the approach of strategic actions. In this work, a diagnosis study of the municipality is carried out, determining the main strengths, weaknesses, threats and opportunities with the corresponding strategies and actions for their improvement. Among the main results of the work is the contribution of 41 866,76 Cuban pesos in a sugar harvest for the contribution of sugar production, final honey, urea honey, cane bagasse for animal food, ice and the electric power generation as a contribution to the national net. Finally, the general procedure used is shown to directives and workers of the sector for the implementation and generalization of the work and its necessary level of consciousness of the value and importance of those productions for the improvement of conditions and quality of life of the citizens of this municipality. For the evaluation of this work the technique of consent index is used among experts.

  14. SOBREVIVENCIA DE Vibrio mimicus EN LA CLARA Y EN LA YEMA DEL HUEVO DE TORTUGA LORA (Lepidochelys olivacea)

    OpenAIRE

    M\\u00F3nica Pereira-Zamora; Eric Wong-Gonz\\u00E1lez

    2008-01-01

    En el año 2000, en el Centro Nacional de Ciencia y Tecnología de Alimentos de la Universidad de Costa Rica, se estudió la sobrevivencia de Vibrio mimicus en la clara y yema del huevo de tortuga lora. Se inocularon muestras de clara o yema con el vibrio (aproximadamente 2,5 x 104 UFC/g) y se determinó la carga inicial y final al cabo de 18 horas de incubación a 25°C. De forma análoga se trabajó una muestra control en agua peptonada alcalina(APA) como medio óptimimo de crecimiento para el vibri...

  15. Superfund Record of Decision (EPA Region 9): Applied Materials, Inc. , Superfund site, Santa Clara, CA. (First remedial action), September 1990

    Energy Technology Data Exchange (ETDEWEB)

    1990-09-28

    The 9-acre Applied Materials site is an active equipment manufacturing facility in Santa Clara, Santa Clara County, California. The site is located within the San Tomas Aquino floodplain, and land use in the area is primarily light industrial, commercial, and residential. Shallow ground water at the site is a potential drinking water source. In 1984 and 1985, as part of interim onsite cleanup activities, Applied Materials excavated and removed underground tanks, piping, and more than 60 cubic yards of contaminated onsite soil, and installed an air stripping unit onsite to treat VOC-contaminated ground water. The Record of Decision (ROD) provides a final remedy for contaminated onsite ground water at the Building 1 area. The primary contaminants of concern affecting the ground water are VOCs including PCE, TCE, and 1,1,1-TCA.

  16. The primitives of Santa Clara of Ubeda: stylistic and iconographic study, critical appraisals and vicissitudes of a dispersed heritage

    Directory of Open Access Journals (Sweden)

    Clara Beltrán Catalán

    2016-12-01

    Full Text Available The authors study the stylistic and iconographic aspects of a collection of paintings on wood dating from the 15th and 16th centuries, originally in the Royal Monastery of Santa Clara at Úbeda. This collection was sold in the 1920s with the participation of the antique dealer Celestino Dupont. The research is complemented by an analysis of the critical appraisals given to these works and their history since their introduction into the art market.

  17. Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.

    Science.gov (United States)

    Pomajbíková, Kateřina; Oborník, Miroslav; Horák, Aleš; Petrželková, Klára J; Grim, J Norman; Levecke, Bruno; Todd, Angelique; Mulama, Martin; Kiyang, John; Modrý, David

    2013-01-01

    Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans.

  18. Unexpected biodiversity of ciliates in marine samples from below the photic zone.

    Science.gov (United States)

    Grattepanche, J. D.; Katz, L. A.; Tucker, S. J.; McManus, G. B.; Santoferrara, L. F.

    2016-02-01

    Marine microbial eukaryotes play critical roles in planktonic food webs and have been described as most diverse in the photic zone where productivity is high. We used high-throughput sequencing (HTS) to analyze the spatial distribution of planktonic ciliate diversity from shallow waters (800m depth) along a 163Km transect off the coast of New England, USA. Using HTS and specific primers, we observed that the most abundant OTUs correspond to those previously-reported elsewhere. However, we did not find the expected decrease of diversity below the photic zone and instead saw an increase of diversity with depth. This highly diversity within samples taken below the photic zone also reveals the prevalence of two `new' clades (i.e. lacking sequenced morphospecies) defined by two deletions within the region of the SSU-rDNA analyzed here. Further, the ciliate communities cluster by layer (e.g. surface, pycnocline) and degree of water column stratification, suggesting that community assembly is driven by environmental factors such as temperature, salinity, phytoplankton and mesozooplankton. Together, these data reinforce that we still have much to learn about microbial diversity in marine ecosystems, especially in deeper waters that may be a reservoir for rare species.

  19. Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.

    Directory of Open Access Journals (Sweden)

    Kateřina Pomajbíková

    Full Text Available Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2. We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans.

  20. Hag mediates adherence of Moraxella catarrhalis to ciliated human airway cells.

    Science.gov (United States)

    Balder, Rachel; Krunkosky, Thomas M; Nguyen, Chi Q; Feezel, Lacey; Lafontaine, Eric R

    2009-10-01

    Moraxella catarrhalis is a human pathogen causing otitis media in infants and respiratory infections in adults, particularly patients with chronic obstructive pulmonary disease. The surface protein Hag (also designated MID) has previously been shown to be a key adherence factor for several epithelial cell lines relevant to pathogenesis by M. catarrhalis, including NCIH292 lung cells, middle ear cells, and A549 type II pneumocytes. In this study, we demonstrate that Hag mediates adherence to air-liquid interface cultures of normal human bronchial epithelium (NHBE) exhibiting mucociliary activity. Immunofluorescent staining and laser scanning confocal microscopy experiments demonstrated that the M. catarrhalis wild-type isolates O35E, O12E, TTA37, V1171, and McGHS1 bind principally to ciliated NHBE cells and that their corresponding hag mutant strains no longer associate with cilia. The hag gene product of M. catarrhalis isolate O35E was expressed in the heterologous genetic background of a nonadherent Haemophilus influenzae strain, and quantitative assays revealed that the adherence of these recombinant bacteria to NHBE cultures was increased 27-fold. These experiments conclusively demonstrate that the hag gene product is responsible for the previously unidentified tropism of M. catarrhalis for ciliated NHBE cells.

  1. Structures, biological activities and phylogenetic relationships of terpenoids from marine ciliates of the genus Euplotes.

    Science.gov (United States)

    Guella, Graziano; Skropeta, Danielle; Di Giuseppe, Graziano; Dini, Fernando

    2010-07-08

    In the last two decades, large scale axenic cell cultures of the marine species comprising the family Euplotidae have resulted in the isolation of several new classes of terpenoids with unprecedented carbon skeletons including the (i) euplotins, highly strained acetylated sesquiterpene hemiacetals; (ii) raikovenals, built on the bicyclo[3.2.0]heptane ring system; (iii) rarisetenolides and focardins containing an octahydroazulene moiety; and (iv) vannusals, with a unique C30 backbone. Their complex structures have been elucidated through a combination of nuclear magnetic resonance spectroscopy, mass spectrometry, molecular mechanics and quantum chemical calculations. Despite the limited number of biosynthetic experiments having been performed, the large diversity of ciliate terpenoids has facilitated the proposal of biosynthetic pathways whereby they are produced from classical linear precursors. Herein, the similarities and differences emerging from the comparison of the classical chemotaxonomy approach based on secondary metabolites, with species phylogenesis based on genetic descriptors (SSU-rDNA), will be discussed. Results on the interesting ecological and biological properties of ciliate terpenoids are also reported.

  2. The fluid dynamics of the ciliate Pseudotontonia sp. jumping by ``tail'' contraction

    Science.gov (United States)

    Jiang, Houshuo; Gemmell, Brad; Buskey, Edward

    2015-11-01

    The marine planktonic ciliate Pseudotontonia sp. (~ 80 μm in cell size) possesses two sets of propulsive machinery: (1) an anteriorly located ciliary band that beats to let the cell swim backward, and (2) a long, contractile appendage (i.e. the `tail') that at times contracts rapidly to pull the cell body backward, resulting in the tail contraction and body jumping motion being oppositely directed inwards towards the same location. We use high-speed microscale imaging and micro-particle image velocimetry techniques to measure the ciliate swimming and jumping kinematics and imposed flow fields. We show that the cilia-propelled swimming achieves a sustained swimming speed ~ 10 mm s-1 that can last more than 100 ms. The swimming imposed flow conforms to the steady stresslet flow field that decays spatially at r-2. On the other hand, the tail contraction causes the cell to jump at a peak speed ~ 55 mm s-1 and cover a jumping distance 2-4 cell lengths within ~ 12 ms jumping time. The jumping imposed flow fits quite well to the unsteady impulsive stokeslet flow field that decays spatially at r-3. Based on the measured jumping kinematics, we develop a fluid dynamics model to explain the thrust generation due to the tail contraction.

  3. Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences.

    Science.gov (United States)

    Pucciarelli, Sandra; Devaraj, Raghul Rajan; Mancini, Alessio; Ballarini, Patrizia; Castelli, Michele; Schrallhammer, Martina; Petroni, Giulio; Miceli, Cristina

    2015-08-01

    We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI's prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in "transmembrane transporter activity" and "oxidoreductase activity". Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment.

  4. Caracterización de las farmacias comunitarias de Santa Clara para la implementación de la Dispensación de Medicamentos Characterization of community pharmacies in Santa Clara city for the implementation of drug dispensing

    Directory of Open Access Journals (Sweden)

    Liset Jaramillo Hernández

    2012-03-01

    Full Text Available Introducción: la inclusión del Servicio de Dispensación de Medicamentos en la Atención Farmacéutica, busca evidenciar la participación del farmacéutico en el logro de resultados positivos en los tratamientos, basado en los conocimientos farmacoterapéuticos y principios éticos que identifican esta profesión. La caracterización de las farmacias comunitarias resulta elemental para trazar acciones que permitan implementar dicho servicio. Objetivo: caracterizar las farmacias comunitarias de Santa Clara para la implementación del Servicio de Dispensación de Medicamentos. Métodos: se realizó una investigación en sistemas y servicios de salud con un enfoque sistémico (evaluación de la estructura, el proceso y los resultados. El estudio fue observacional, descriptivo y transversal. El contexto espacial lo constituyeron las farmacias comunitarias del municipio de Santa Clara, en el período comprendido entre enero y mayo de 2010. Se seleccionó el 30 % del total de farmacias comunitarias del municipio de Santa Clara. Resultados: la disponibilidad de los recursos humanos fue limitada y no existieron suficientes recursos materiales para sustentar el servicio. El 95,2 % de los farmacéuticos comunitarios presentaron altas necesidades de aprendizaje sobre Dispensación y no existieron Procedimientos Normalizados de Trabajo. A través de la observación directa se determinó que el servicio no se ejecutó, lo cual se corroboró con la encuesta realizada a los usuarios. Conclusiones: las farmacias comunitarias del municipio de Santa Clara se caracterizan por tener una estructura parcialmente adecuada y por ser el proceso y los resultados no adecuados para la ejecución del Servicio de Dispensación de Medicamentos.Introduction: the drug dispensing service as part of the pharmaceutical care is aimed at showing the involvement of the pharmacist in attaining positive results of therapies, based on the pharmaco-therapeutic knowledge and the

  5. A Bone Health Intervention for Chinese Immigrants in Santa Clara County.

    Science.gov (United States)

    Zou, Joanne; Hampton, Michelle DeCoux; Shade, Kate; Kaku, Leonard

    Among Chinese immigrants, osteoporosis is undertreated, misdiagnosed, and a leading cause of fragility fractures. In orthopaedic surgery departments, prevention education and health behavior change programs are necessary to improve their bone health. The purpose of this study was to examine the effectiveness of an osteoporosis prevention education program on participants' self-efficacy with regard to exercise and nutrition when provided by an orthopaedic surgery team during an annual Chinese Health Fair in Santa Clara County, CA. This pilot study used a single-group pretest and posttest design. Chinese immigrants at risk of osteoporosis were recruited during a 1-day health fair. The Bone Health Intervention (BHI) included orthopaedic surgeon consultation, visual aids including osteoporosis images and bone models, a video that included a discussion on calcium and vitamin D in the Chinese diet and culturally-acceptable exercise, and osteoporosis educational handouts. The Osteoporosis Self-Efficacy Scale (OSES) was utilized to measure participants' confidence in the ability to participate in self-care behaviors related to physical activity and calcium intake before and after the intervention. Paired t tests were used to compare participants' OSES scores pre- and postintervention. There was a significant increase in mean OSES scores postintervention, indicating that the intervention could be an effective method of increasing participants' self-efficacy regarding calcium intake and time spent in exercising. These results indicate that a culturally meaningful education program can potentially reduce fragility fracture risk. Orthopaedic health providers are ideal candidates to deliver preventive care education to improve outcomes for Chinese immigrants.

  6. Increased fucosyl glycoconjugate by Mycoplasma hyopneumoniae enhances adherences of Pasteurella multocida type A in the ciliated epithelial cells of the respiratory tract.

    Science.gov (United States)

    Park, Changhoon; Jeong, Jiwoon; Kang, Ikjae; Choi, Kyuhyung; Park, Su-Jin; Chae, Chanhee

    2016-02-03

    The objective of this study was to elucidate the pathogenic mechanisms of how Mycoplasma hyopneumoniae enhances secondary Pasteurella multocida type A infection which leads to porcine enzootic pneumonia in infected pigs. Sixteen pigs were experimentally infected with M. hyopneumoniae and then euthanized at 7, 14, 21 and 28 days post inoculation. In situ hybridization for M. hyopneumoniae DNA and Ulex europaeus agglutinin-I (UEA-I) lectin histochemistry for fucosyl glycoconjugate, was performed in serial lung sections to determine alteration of fucosyl glycoconjugate in M. hyopneumoniae-infected bronchial and bronchiolar epithelium. Bacterial overlay assay was performed to determine the affinity of P. multocida type A with L-fucose. The luminal surface of bronchial and bronchiolar epithelial cells that were stained with UEA-I always showed hybridization signals for M. hyopneumoniae but it was negative in the unaffected parts of the lung from M. hyopneumoniae-infected pigs and in lung from negative control pigs. Colocalization of M. hyopneumoniae and UEA-I was especially prominent in the luminal surface of bronchial and bronchiolar epithelial cells in serial section of lung. The mean number of M. hyopneumoniae-positive cells correlated with the mean number of UEA-I-positive cells in lungs from infected pigs throughout the experiment. All eight P. multocida type A isolates from naturally occurring enzootic pneumonia, bound strongly at levels of 2 μg and 5 μg of L-fucose. The results of the present study demonstrate that M. hyopneumoniae increases the L-fucose composition to enhance adherence of P. multocida type A to the bronchial and bronchiolar epithelial cells.

  7. Transcriptional program of ciliated epithelial cells reveals new cilium and centrosome components and links to human disease.

    Directory of Open Access Journals (Sweden)

    Ramona A Hoh

    Full Text Available Defects in the centrosome and cilium are associated with a set of human diseases having diverse phenotypes. To further characterize the components that define the function of these organelles we determined the transcriptional profile of multiciliated tracheal epithelial cells. Cultures of mouse tracheal epithelial cells undergoing differentiation in vitro were derived from mice expressing GFP from the ciliated-cell specific FOXJ1 promoter (FOXJ1:GFP. The transcriptional profile of ciliating GFP+ cells from these cultures was defined at an early and a late time point during differentiation and was refined by subtraction of the profile of the non-ciliated GFP- cells. We identified 649 genes upregulated early, when most cells were forming basal bodies, and 73 genes genes upregulated late, when most cells were fully ciliated. Most, but not all, of known centrosome proteins are transcriptionally upregulated early, particularly Plk4, a master regulator of centriole formation. We found that three genes associated with human disease states, Mdm1, Mlf1, and Dyx1c1, are upregulated during ciliogenesis and localize to centrioles and cilia. This transcriptome for mammalian multiciliated epithelial cells identifies new candidate centrosome and cilia proteins, highlights similarities between components of motile and primary cilia, and identifies new links between cilia proteins and human disease.

  8. Meiosis gene inventory of four ciliates reveals the prevalence of a synaptonemal complex-independent crossover pathway.

    Science.gov (United States)

    Chi, Jingyun; Mahé, Frédéric; Loidl, Josef; Logsdon, John; Dunthorn, Micah

    2014-03-01

    To establish which meiosis genes are present in ciliates, and to look for clues as to which recombination pathways may be treaded by them, four genomes were inventoried for 11 meiosis-specific and 40 meiosis-related genes. We found that the set of meiosis genes shared by Tetrahymena thermophila, Paramecium tetraurelia, Ichthyophthirius multifiliis, and Oxytricha trifallax is consistent with the prevalence of a Mus81-dependent class II crossover pathway that is considered secondary in most model eukaryotes. There is little evidence for a canonical class I crossover pathway that requires the formation of a synaptonemal complex (SC). This gene inventory suggests that meiotic processes in ciliates largely depend on mitotic repair proteins for executing meiotic recombination. We propose that class I crossovers and SCs were reduced sometime during the evolution of ciliates. Consistent with this reduction, we provide microscopic evidence for the presence only of degenerate SCs in Stylonychia mytilus. In addition, lower nonsynonymous to synonymous mutation rates of some of the meiosis genes suggest that, in contrast to most other nuclear genes analyzed so far, meiosis genes in ciliates are largely evolving at a slower rate than those genes in fungi and animals.

  9. Draft Genome Sequence of Holospora undulata Strain HU1, a Micronucleus-Specific Symbiont of the Ciliate Paramecium caudatum.

    Science.gov (United States)

    Dohra, Hideo; Suzuki, Haruo; Suzuki, Tomohiro; Tanaka, Kenya; Fujishima, Masahiro

    2013-08-22

    Holospora undulata is a micronucleus-specific symbiont of the ciliate Paramecium caudatum. We report here the draft genome sequence of H. undulata strain HU1. This genome information will contribute to the study of symbiosis between H. undulata and the host P. caudatum.

  10. A simplified experimental model for clearance of some pathogenic bacteria using common bacterivorous ciliated spp. in Tigris river

    Science.gov (United States)

    Ali, Talib Hassan; Saleh, Dhuha Saad

    2014-03-01

    Bacteria-specific uptake rates of three different protozoan taxa on a pure and mixed bacterial community was studied by means of a simplified and functionally reproducible experimental model. The bacterial species Shigella flexneri, Escherichia coli and Salmonella typhi were isolated and classified from stool samples of patients suffering from diarrhea. Paramecium caudatum, Tetrahymena pyriformis and Halteria grandinella, free living ciliate Protozoans, were isolated and identified from Tigris river water. Pure and mixed ( E. coli + S. typhi), ( E. coli + Sh. flexneri) bacterial cultures were used with each ciliate genera to evaluate the following: predator duplication rate, prey reduction rate, clearance rate and net grazing rate. We used selective lactose fermentation phenomena of enteric bacteria on MacConkey medium for the quantification of bacteria cultural characteristics. The final bacteria concentration was reduced by growing protozoa of 98-99.9 % compared to protozoa-free controls. It showed that Tetrahymena pyriformis had the highest duplication rate (4.13 time/day) in both types of cultures (pure and mixed), followed by Paramecium caudatum and Halteria grandinella, respectively. Paramecium caudatum had the highest rate of ingestion in both types of cultures (26 × 103 bacteria/organism/hr) and yielded the longest time required for 90 % bacterial reduction in a pure suspension of S. typhi (166 h). Clearance rates of pathogenic bacteria by ciliates ranged between 106 nanoliter/organism/h by P. caudatum to S. typhi and 1.92 nanoliter/organism/h seen in T. pyriformis in ( E. coli + S. typhi) mixed culture. We used aquatic experimental microcosms under controlled conditions to explore bacteria-dependent ciliate growth and examined whether these ciliates could discriminate between equally sized bacterial preys in a mixture.

  11. Spatiotemporal variation in community structure of marine benthic ciliates in the Yellow Sea during and after macroalgal and giant jellyfish blooms

    Science.gov (United States)

    Zhou, Bailing; Xu, Kuidong

    2016-07-01

    The annual bloom of the green macroalgal Ulva prolifera from May through July since 2008 and another of giant jellyfish Nemopilema nomurai from June through September have been frequent events in the Yellow Sea. However, the patterns of benthic ciliate communities during and after the blooms are still not known. In combination with analyses of benthic environmental factors, we investigated the distribution and community composition of benthic ciliates in the Yellow Sea in July and November 2011. In July, ciliates had high standing crops and diversity in the northern Yellow Sea, and in the inshore area off the southern Shandong Peninsula, where large numbers of green macroalgae accumulated. In November, the abundance, biomass and diversity of ciliates were high in the sea areas off the Shandong Peninsula and Changjiang estuary, where a large quantity of jellyfish occurred in August. Neither the abundance nor the biomass had significant difference between seasons, or between different compartments of the Yellow Sea. The species number, and both Margalef and Shannon-Wiener indices of ciliates were all significantly higher in November than in July. In both seasons, prostomateans and karyorelicteans consistently constituted the first and second most important ciliate groups in biomass; and carnivorous ciliates constituted the primary feeding type in terms of biomass as well as species richness, followed by bacterivores, algivores and omnivores. Compared with that in June 2007 when no macroalgae occurred, the percentage of small-sized bacterivores (e.g. Metacystis spp., Euplotes spp. and scuticociliates) increased in July 2011. The proportion of carnivorous ciliates increased in November, and this increased dominance of carnivorous ciliates may be a response to the increase in predominance of heterotrophic nanoflagellates, which might in turn be ascribed to an effect of green macroalgal and giant jellyfish blooms in the Yellow Sea.

  12. Política social para la atención a la vejez: una necesidad para. Villa Clara Social policy for old age care: a necessity for Villa Clara

    Directory of Open Access Journals (Sweden)

    Yamila Roque Doval

    2015-12-01

    Full Text Available Resumen Villa Clara es la provincia más envejecida del país y la atención a este proceso demográfico se declara como urgente para este territorio. El artículo que se presenta constituye un resultado parcial del estudio que viene realizando, en el área de Política Social, un grupo de investigadores del proyecto Estudios de Población, que coordina el Centro de Estudios Demográficos (CEDEM, en la Universidad Central “Marta Abreu” de Las Villas. El objetivo del estudio es destacar, a partir del análisis de datos estadísticos del Censo de Población y Viviendas del 2012 ofrecidos por la Oficina Nacional de Estadísticas e Información (ONEI, la urgencia de incorporar a la estrategia de desarrollo de la provincia de Villa Clara la necesidad de la atención al adulto mayor. La muestra seleccionada está compuesta por 8 municipios de la provincia (Quemado de Güines, Sagua la Grande, Encrucijada, Cifuentes, Santa Clara, Placetas, Camajuaní y Remedios, que son los más envejecidos, según datos de la ONEI. Como resultado se propone que: la política social que se diseñe tenga como objetivo general lograr una atención eficaz a las necesidades del adulto mayor de la provincia a partir de los rasgos esenciales que caracterizan ese proceso en el territorio, que se base en valores como la solidaridad, la cooperación, que los principios que la sustenten sean de descentralización, flexibilidad en el diseño de la política, prevención, equidad, valorando siempre las necesidades y las posibilidades reales de acceso a los servicios de este grupo poblacional. Villa Clara provinceis the oldest in the country and attention to this demographic processis declared a surgent for this territory. The present article is a partial result of the study being done in the area of Social Policy, a group of researchers at the Population Studies project, coordinated by CEDEM at the Central University “Marta Abreu” of Las Villas. The aim of the studyis to

  13. Produção fora de época da videira 'BRS Clara' sob cultivo protegido Out of season production of 'BRS Clara' seedless grape under protected cultivation

    Directory of Open Access Journals (Sweden)

    Larissa Abgariani Colombo

    2011-02-01

    Full Text Available No norte do Paraná, a viticultura diferencia-se de outras regiões produtoras, pois são obtidas, nessa região, duas safras em um ano, uma regular e outra fora de época. Entretanto, o clima quente e úmido da região favorece a ocorrência do míldio (Plasmopara viticola e uma alternativa para minimizar esse problema é o emprego do sistema de produção de uvas sob cultivo protegido. O trabalho teve como objetivo avaliar a produção fora de época da videira 'BRS Clara' sobre os porta-enxertos 'IAC 572 Jales' e 'IAC 766 Campinas', submetida à diferentes tipos de cultivo protegido. O delineamento experimental empregado foi o de blocos ao acaso com quatro repetições, em arranjo fatorial 7x2 (sete tipos de cultivo protegido e dois porta-enxertos. Os tratamentos foram: a. sombrite sem fungicidas para míldio; b. sombrite com fungicidas para míldio (padrão de controle da região; c. cobertura plástica sem fungicidas para míldio; d. cobertura plástica e 50% de redução do padrão de fungicidas para míldio; e. cobertura plástica e 75% de redução do padrão de fungicidas para míldio; f. cobertura plástica com fosfito e cobre; e g. cobertura plástica sem fungicidas. Os porta-enxertos 'IAC 766' e 'IAC 572' são indicados para a produção da uva sem semente 'BRS Clara' na região norte do Paraná; o emprego da cobertura plástica permite a redução de até 75% do número de aplicações de fungicidas no cultivo da uva 'BRS Clara', mas essa cobertura, por si só, não é suficiente para prevenir a ocorrência de míldio nos cachos, em condições de umidade elevada.The viticulture of the north of Parana state differs from other regions, because in this area two crops are obtained a year, a regular and an out of season ones. However, the hot and humid climate of the region favors the occurrence of downy mildew (Plasmopara viticola, an alternative to minimize this problem is the use of grape production under protected cultivation. The

  14. Ciliated Median Raphe Cyst of Perineum Presenting as Perianal Polyp: A Case Report with Immunohistochemical Study, Review of Literature, and Pathogenesis

    Directory of Open Access Journals (Sweden)

    Jayesh Sagar

    2006-01-01

    Full Text Available Median raphe cyst is a very rare, benign congenital lesion occurring mainly on the ventral aspect of the penis, but can develop anywhere in the midline between the external urethral meatus and anus. We report a case of median raphe cyst in the perineum presenting as a perianal polyp in a 65-year-old, English white male with exceptionally rare ciliated epithelium. According to our knowledge, this is the third such case of ciliated median raphe cyst in the English literature. This case, also the first case of ciliated median raphe cyst in the perineum location, focuses on pathogenesis of median raphe cyst.

  15. New Record of Two Marine Ciliates (Ciliophora: Spirotrichea in South Korea

    Directory of Open Access Journals (Sweden)

    Kang-San Kim

    2013-04-01

    Full Text Available Two marine hypotrichous ciliates, Anteholosticha petzi and Ponturostyla enigmatica, were collected from the Yellow Sea and the Korea Strait, respectively, and described using live observation and protargol-impregnated specimens. Furthermore, the nuclear small subunit ribosomal RNA gene of each was sequenced and compared to previously annotated sequences retrieved from the GenBank. Anteholosticha petzi is characterized by 3 frontal cirri (FC, 2 frontoterminal cirri (FTC, 8-12 transverse cirri (TC, 1 buccal cirrus (BC, 9-12 midventral pairs (MP, 3 bipolar dorsal kineties (DK, and 3 types of colorless cortical granules. Ponturostyla enigmatica is characterized by 8 FC, 5 ventral cirri (VC, 5-7 TC, 6-7 marginal rows (MR on each side, 4 complete and 2-3 partial DK, and greenish cortical granules. This is the first identification and description of these 2 species, A. petzi and P. enigmatica, in South Korea.

  16. Epibiotic ciliates Scyphidia sp. and diatoms on Tigriopus fulvus (Copepoda: Harpacticoida exoskeleton

    Directory of Open Access Journals (Sweden)

    Luigi Pane

    2014-12-01

    Full Text Available Several microorganisms – epibionts – can adhere to living supports taking advantage for their survival, feeding and movement. Epibiosis occurs particularly in aquatic environments, on both benthic and planktonic organisms, among which copepods and cladocerans represent an important living support. The harpacticoid copepod Tigriopus fulvus, living in the splashpools of rocky coasts, was studied to recognize the occurrence of epibionts on the exoskeleton surface using scanning electon microscopy techniques. The first evidence of ciliate Scyphidia sp. on Tigriopus fulvus has been described and the occurrence of algae Cocconeis sp. has been observed as well. Epibionts were found to adhere to antennae, a site linked to the exploitation of water currents carrying food particles to mouthparts and to swimming legs. The reason of the occurrence on swimming legs is less clear and needs further observations. Pertinent results are described and discussed and the influence of epibionts on life cycle and behavior of Tigriopus fulvus is considered.

  17. In vitro treatments for the theront stage of the ciliate protozoan Cryptocaryon irritans.

    Science.gov (United States)

    Picón-Camacho, S M; Ruiz de Ybáñez, M R; Holzer, A S; Arizcun Arizcun, M; Muñoz, P

    2011-04-06

    The ciliate protozoan Cryptocaryon irritans Brown, 1951, the 'marine white spot', causes one of the most important parasitic fish diseases, with extensive losses every year in mariculture and in the ornamental fish industry. In the present study, we explore the in vitro use of 8 different compounds against the theront (infective) stage of C. irritans; these compounds include extracts of natural products (epigallocatechin gallate (EGCG), L-DOPA, papain), peracetic acid-based compounds (Proxitane 5:23 and 15% peracetic acid, PAA), quinine-based compounds (quinacrine hydrochloride and chloroquine diphosphate) and hydrogen peroxide. All of these compounds had an effect on theront survival; however, only EGCG caused significant theront mortality when applied in doses > or =50 mg l(-1) and over a period of 3 h; papain caused a maximum theront mortality of irritans infections in marine aquaculture and the ornamental fish industry.

  18. Population dynamics of active and total ciliate populations in arable soil amended with wheat

    DEFF Research Database (Denmark)

    Ekelund, F.; Frederiksen, Helle B.; Ronn, R.

    2002-01-01

    of the population may be encysted. The factors governing the dynamics of active and encysted cells in the soil are not well understood. Our objective was to determine the dynamics of active and encysted populations of ciliates during the decomposition of freshly added organic material. We monitored, in soil......Soil protozoa are characterized by their ability to produce cysts, which allows them to survive unfavorable conditions (e.g., desiccation) for extended periods. Under favorable conditions, they may rapidly excyst and begin feeding, but even under optimal conditions, a large proportion...... why protozoan growth in soil is less than that in aquatic systems. Internally governed encystment may be an essential adaptation to an unpredictable environment in which individual protozoa cannot predict when the soil will dry out and will survive desiccation only if they have encysted in time....

  19. Physical hydrodynamic propulsion model study on creeping viscous flow through a ciliated porous tube

    Science.gov (United States)

    Akbar, Noreen Sher; Butt, Adil Wahid; Tripathi, Dharmendra; Bég, O. Anwar

    2017-03-01

    The present investigation focusses on a mathematical study of creeping viscous flow induced by metachronal wave propagation in a horizontal ciliated tube containing porous media. Creeping flow limitations are imposed, i.e. inertial forces are small compared to viscous forces and therefore a very low Reynolds number (Re ≪ 1) is taken into account. The wavelength of metachronal wave is also considered to be very large for cilia movement. The physical problem is linearized and exact solutions are developed for the differential equation problem. Mathematica software is used to compute and illustrate numerical results. The influence of slip parameter and Darcy number on velocity profile, pressure gradient and trapping of bolus are discussed with the aid of graphs. It is found that with increasing magnitude of the slip parameter, the trapped bolus inside the streamlines increases in size. The study is relevant to biological propulsion of medical micromachines in drug delivery.

  20. Dependence between volumes of protoplast and lorica in lugol-fixed tintinnid ciliates.

    Science.gov (United States)

    Rychert, Krzysztof

    2011-04-01

    The relationship between the volume of tintinnid loricae and protoplasts was studied on the basis of bulk data derived from samples collected in a range of temperate environments: riverine, lacustrine, estuarine, and marine. All organisms were fixed with acid Lugol solution (0.5-1.0%). The dependence was described with the allometric equation: VL=2.74×VP(1.03), where VL and VP are volumes [μm(3)] of loricae and protoplasts, respectively. The dependence was highly significant (p<0.001, n=417, R(2)=0.85). On average, protoplasts occupied 31% of the lorica volume. One species, Helicostomella subulata,was omitted from the calculations and treated separately since this ciliate has a relatively large lorica. The relationship identified between lorica and protoplast volumes permits recalculating incomplete data if protoplasts are the only body part measured. Copyright © 2010 Elsevier GmbH. All rights reserved.

  1. Effect of Cypermethrin on the Growth of Ciliate Protozoan Paramecium caudatum.

    Science.gov (United States)

    Dutta, Joydeep

    2015-01-01

    The objective of this study is to assess the effect of cypermethrin on the growth of ciliate protozoan Paramecium caudatum. Monoxenic culture of P. caudatum, were exposed to different doses (0.01, 0.05, 0.1, 0.15, and 0.2 µg/L) of cypermethrin along with control for 24, 48, 72, and 96 h time interval. The total numbers of live and dead cells were counted after trypan blue staining in Neubauer hemocytometer. Marked decrease in the number of living cells with the increase in the concentration of cypermethrin and with increasing exposure time intervals was recorded. The results indicate that cypermethrin is toxic to P. caudatum even at low concentrations when it enters in the aquatic system through runoff.

  2. Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes

    Directory of Open Access Journals (Sweden)

    Kurt Wüthrich

    2013-01-01

    Full Text Available Three psychrophilic protein pheromones (En-1, En-2 and En-6 from the polar ciliate, Euplotes nobilii, and six mesophilic pheromones (Er-1, Er-2, Er-10, Er-11, Er-22 and Er-23 from the temperate-water sister species, Euplotes raikovi, were studied in aqueous solution for their thermal unfolding and refolding based on the temperature dependence of their circular dichroism (CD spectra. The three psychrophilic proteins showed thermal unfolding with mid points in the temperature range 55–70 °C. In contrast, no unfolding was observed for any of the six mesophilic proteins and their regular secondary structures were maintained up to 95 °C. Possible causes of these differences are discussed based on comparisons of the NMR structures of the nine proteins.

  3. Nuclear Architecture and Patterns of Molecular Evolution Are Correlated in the Ciliate Chilodonella uncinata.

    Science.gov (United States)

    Maurer-Alcalá, Xyrus X; Katz, Laura A

    2016-06-08

    The relationship between nuclear architecture and patterns of molecular evolution in lineages across the eukaryotic tree of life is not well understood, partly because molecular evolution is traditionally explored as changes in base pairs along a linear sequence without considering the context of nuclear position of chromosomes. The ciliate Chilodonella uncinata is an ideal system to address the relationship between nuclear architecture and patterns of molecular evolution as the somatic macronucleus of this ciliate is composed of a peripheral DNA-rich area (orthomere) and a DNA-poor central region (paramere) to form a "heteromeric" macronucleus. Moreover, because the somatic chromosomes of C. uncinata are highly processed into "gene-sized" chromosomes (i.e., nanochromosomes), we can assess fine-scale relationships between location and sequence evolution. By combining fluorescence microscopy and analyses of transcriptome data from C. uncinata, we find that highly expressed genes have the greatest codon usage bias and are enriched in DNA-poor regions. In contrast, genes with less biased sequences tend to be concentrated in DNA abundant areas, at least during vegetative growth. Our analyses are consistent with recent work in plants and animals where nuclear architecture plays a role in gene expression. At the same time, the unusual localization of nanochromosomes suggests that the highly structured nucleus in C. uncinata may create a "gene bank" that facilitates rapid changes in expression of genes required only in specific life history stages. By using "nonmodel" organisms like C. uncinata, we can explore the universality of eukaryotic features while also providing examples of novel properties (i.e., the presence of a gene bank) that build from these features. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  4. Signalling in ciliates: long- and short-range signals and molecular determinants for cellular dynamics.

    Science.gov (United States)

    Plattner, Helmut

    2017-02-01

    In ciliates, unicellular representatives of the bikont branch of evolution, inter- and intracellular signalling pathways have been analysed mainly in Paramecium tetraurelia, Paramecium multimicronucleatum and Tetrahymena thermophila and in part also in Euplotes raikovi. Electrophysiology of ciliary activity in Paramecium spp. is a most successful example. Established signalling mechanisms include plasmalemmal ion channels, recently established intracellular Ca(2+) -release channels, as well as signalling by cyclic nucleotides and Ca(2+) . Ca(2+) -binding proteins (calmodulin, centrin) and Ca(2+) -activated enzymes (kinases, phosphatases) are involved. Many organelles are endowed with specific molecules cooperating in signalling for intracellular transport and targeted delivery. Among them are recently specified soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), monomeric GTPases, H(+) -ATPase/pump, actin, etc. Little specification is available for some key signal transducers including mechanosensitive Ca(2+) -channels, exocyst complexes and Ca(2+) -sensor proteins for vesicle-vesicle/membrane interactions. The existence of heterotrimeric G-proteins and of G-protein-coupled receptors is still under considerable debate. Serine/threonine kinases dominate by far over tyrosine kinases (some predicted by phosphoproteomic analyses). Besides short-range signalling, long-range signalling also exists, e.g. as firmly installed microtubular transport rails within epigenetically determined patterns, thus facilitating targeted vesicle delivery. By envisaging widely different phenomena of signalling and subcellular dynamics, it will be shown (i) that important pathways of signalling and cellular dynamics are established already in ciliates, (ii) that some mechanisms diverge from higher eukaryotes and (iii) that considerable uncertainties still exist about some essential aspects of signalling. © 2015 Cambridge Philosophical Society.

  5. Automatización de la red de 34,5kV en la provincia de Villa Clara; Automation of 34,5 kV network in the province of Villa Clara

    Directory of Open Access Journals (Sweden)

    Obedi Álvarez Díaz

    2012-11-01

    Full Text Available En la actualidad la operación de la redes de 34.5kV de la provincia de Villa Clara se hace muy complejo debido a que los desconectivos existentes son operados manualmente por el personal y los tiempos de operación son extensos. El objetivo de la implementación de la automatización de las redes de 34.5kV en Villa Clara es operar dicha red de la forma más eficiente posible, donde se le suministre la energía eléctrica a los clientes con mínimos costos de operación, alto nivel de confiabilidad, disminución de la frecuencia de interrupciones y también de los tiempos. Se seleccionaron los lazos más importantes de la provincia, los cuales incluyen generación distribuida, realizándoles corridas de flujo de carga usando el software PowerSystem Explorer (PSX, obteniendo los lugares donde se deben colocar los recerradores. Estos se comunicarán entre sí, pudiendo ser configurables para distintas condiciones, además de poder operarlos a distancia.  At present the operation of the 34.5kV network of the province of Villa Clara is very complex because the existing disconnected are operated by staff and operating times are long. The objective of the implementation of the automation 34.5 kV networks in Villa Clara is operate as efficiently way as possible, where you supply the electricity to customers with minimal operating costs, high reliability, reduced the frequency of interruptions and the times. There have been selected the most important loops in the province, which include distributed generation, performing load flow runs using the Power System Explorer software(PSX, obtaining the locations should be placed reclosing. They shall communicate with each otherand can be configured for different conditions, in addition to being to operate them at a distance.

  6. Cronología de emergencia de la dentición permanente en niños del municipio de Santa Clara: Parte I Permanent dentition emergence chronology in children from Santa Clara municipality: Part I

    Directory of Open Access Journals (Sweden)

    Armando San Miguel Pentón

    2011-09-01

    Full Text Available Se realizó un estudio epidemiológico descriptivo transversal en Santa Clara desde el año 2005 al año 2009, con el objetivo de determinar la cronología de emergencia de la dentición permanente en niños y niñas del municipio Santa Clara y establecer las diferencias con relación a los valores ofrecidos para otras poblaciones. El universo fue de 45 724 infantes de ambos sexos entre los 4 y los 14 años de edad, con una muestra de 1 769 individuos. Se obtuvieron los valores medios y se estableció la cronología a partir del 50 percentil. Se compararon los promedios de erupción obtenidos con los ofrecidos por otros autores. Se determinó la edad de emergencia de los dientes permanentes en la muestra estudiada. Hubo adelanto cronológico en: incisivos, caninos superiores, primeros molares inferiores y segundos bicúspides inferiores, retardo para segundos bicúspides superiores y caninos inferiores en relación con los autores seleccionados para la comparación. La cronología de emergencia obtenida difiere de los valores ofrecidos para otras poblaciones. La mayor coincidencia resultó con valores de Logan y Kronfeld. La cronología de emergencia difiere entre los sexos.A cross-sectional, descriptive and epidemiologic study was conducted of permanent dentition of children from Santa Clara municipality between 2005 and 2009. To determine the permanent dentition emergence chronology in children (boys and girls from the Santa Clara municipality and to establish the differences in relation to values offered by other populations. The universe included 45 724 infants of both sexes aged between 4 and 14 with a sample of 1 769 subjects. Mean values were obtained establishing the chronology from the 50 percentile comparing the obtained eruption averages with that offered by other authors. The age of permanent teeth emergence was determined in study sample. There was a chronologic advance in: incisors, upper canines, first lower molars and second

  7. A Contamination Vulnerability Assessment for the Santa Clara and San Mateo County Groundwater Basins

    Energy Technology Data Exchange (ETDEWEB)

    Moran, J E; Hudson, G B; Eaton, G F; Leif, R

    2004-01-06

    In response to concerns expressed by the California Legislature and the citizenry of the State of California, the State Water Resources Control Board (SWRCB), implemented a program to assess groundwater quality, and provide a predictive capability for identifying areas that are vulnerable to contamination. The program was initiated in response to concern over public supply well closures due to contamination by chemicals such as MtBE from gasoline, and solvents from industrial operations. As a result of this increased awareness regarding groundwater quality, the Supplemental Report of the 1999 Budget Act mandated the SWRCB to develop a comprehensive ambient groundwater-monitoring plan, and led to the initiation of the Ambient Groundwater Monitoring and Assessment (GAMA) Program. The primary objective of the GAMA Program is to assess the water quality and to predict the relative susceptibility to contamination of groundwater resources throughout the state of California. Under the GAMA program, scientists from Lawrence Livermore National Laboratory (LLNL) collaborate with the SWRCB, the U.S. Geological Survey, the California Department of Health Services (DHS), and the California Department of Water Resources (DWR) to implement this groundwater assessment program. In 2001 and 2002, LLNL carried out this vulnerability study in the groundwater basins of Santa Clara County and San Mateo County, located to the south of the city of San Francisco. The goal of the study is to provide a probabilistic assessment of the relative vulnerability of groundwater used for the public water supply to contamination from surface sources. This assessment of relative contamination vulnerability is made based on the results of two types of analyses that are not routinely carried out at public water supply wells: ultra low-level measurement of volatile organic compounds (VOCs), and groundwater age dating (using the tritium-helium-3 method). In addition, stable oxygen isotope measurements

  8. Personal exposure to fine particulate matter, lung function and serum club cell secretory protein (Clara).

    Science.gov (United States)

    Wang, Cuicui; Cai, Jing; Chen, Renjie; Shi, Jingjin; Yang, Changyuan; Li, Huichu; Lin, Zhijing; Meng, Xia; Liu, Cong; Niu, Yue; Xia, Yongjie; Zhao, Zhuohui; Li, Weihua; Kan, Haidong

    2017-06-01

    The underlying mechanisms about the association between ambient fine particulate matter (PM 2.5 ) and lung function were unclear. Few epidemiological studies have evaluated the potential mediating effects of serum club cell secretory protein (Clara) (CC16), a biomarker of pulmonary epithelium integrity. To evaluate the short-term effect of personal PM 2.5 exposure on lung function and to explore the potential mediating role of CC16 in this effect. We enrolled 36 healthy, nonsmoking college students for a panel study in Shanghai, China from December 17, 2014 to July 11, 2015. We measured personal and real-time exposure to PM 2.5 for 72 h preceding each of four rounds of health examinations, including lung function test and serum CC16 measurement. We used linear mixed-effect models to examine the effects of PM 2.5 on lung function and CC16 over various lag times. Furthermore, we analyzed the mediating effect of CC16 in the association between PM 2.5 and lung function. Average PM 2.5 exposure ranged from 36 to 52 μg/m3 across different lag periods. PM 2.5 exposure was negatively associated with lung function and positively associated with serum CC16 concentration. The effect of PM 2.5 on CC16 occurred earlier than that on lung function. For instance, an interquartile range (IQR) increase in 0-2 h average exposure to PM 2.5 was significantly associated with a 4.84% increase in serum CC16; and an IQR increase in 3-6 h average exposure to PM 2.5 was significantly associated with a 1.08% decrease in 1-sec forced expiratory volume. These effects lasted up to 24 h after exposure. Increased serum CC16 contributed 3.9%-36.3% of the association between PM 2.5 and impaired lung function. Acute exposure to PM 2.5 might induce an immediate decrease in lung function by virtue of the loss of pulmonary epithelium integrity. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Evaluación de la satisfacción del cliente de la Drogueria Villa Clara, 2008-2009

    Directory of Open Access Journals (Sweden)

    Geidy Lorezo Monteagudo

    2012-03-01

    Full Text Available Introducción: la Droguería Villa Clara es la encargada de la distribución de medicamentos, reactivos y otros insumos médicos en esta provincia de Cuba. Mantiene relaciones comerciales con gran variedad de clientes, pero los más importantes son los pertenecientes al Sistema de Salud. Objetivo: evaluar la calidad del servicio brindado por esta entidad a los hospitales, policlínicos y farmacias de la provincia durante el 2008 y el 2009. Métodos: se conformó una muestra representativa de estas instituciones en todos los municipios de Villa Clara y se aplicaron encuestas al personal responsable de medicamentos en cada tipo unidad. Se determinó el índice de satisfacción para cada cliente encuestado en particular, así como para policlínicos, hospitales y farmacias en sentido general. Por otra parte, se determinó el índice de satisfacción alcanzado de forma global por la droguería en cada año evaluado. Resultados: el servicio brindado por la Droguería Villa Clara durante el 2008 y el 2009 fue calificado como bueno, con una mejora palpable al obtenerse índices de satisfacción que variaron de 84,2 % en el 2008 a 88,3 % en el 2009. Conclusiones: los clientes del proceso de distribución de medicamentos, perciben la disponibilidad de medicamentos como el problema fundamental de la distribución en la provincia.

  10. Características físico-químicas e produtivas da videira 'BRS Clara' sob cultivo protegido

    Directory of Open Access Journals (Sweden)

    Larissa Abgariani Colombo

    2011-09-01

    Full Text Available Atualmente, a preferência por uvas sem sementes vem aumentando nos mercados interno e externo, sendo uma alternativa a produção de uvas sob cultivo protegido. No entanto, o custo de produção de uvas finas para mesa tem sido afetado pela intensa necessidade de controle de doenças fúngicas, como o míldio (Plasmopara viticola. O trabalho teve como objetivo avaliar a produção e as características físicoquímicas dos frutos da videira 'BRS Clara' sobre os porta-enxertos 'IAC 572 Jales' e 'IAC 766 Campinas', sob diferentes tipos de cultivo protegido. O experimento foi realizado no município de Marialva-PR, durante duas safras regulares (set.-dez.2007, ago-dez.2008. O delineamento experimental foi o de blocos ao acaso, com quatro repetições, em arranjo fatorial 7 x 2 (sete tipos de cultivo protegido e dois porta-enxertos. Os tratamentos foram os seguintes tipos de cultivo: a. tela plástica sem fungicidas para míldio; b. tela plástica com fungicidas para míldio (padrão de controle da região; c. cobertura plástica sem fungicidas para míldio; d. cobertura plástica e 50% de redução do padrão de fungicidas para míldio; e. cobertura plástica e 75% de redução do padrão de fungicidas para míldio; f. cobertura plástica com fosfito e cobre, e g. cobertura plástica sem fungicidas. Verificou-se que o cultivo protegido não alterou as características produtivas da videira 'BRS Clara', como número de cachos e produção por planta; os porta-enxertos 'IAC 766' e 'IAC 572' são indicados para a produção da uva 'BRS Clara', e a utilização da cobertura plástica permite a redução do número de aplicações de fungicidas para míldio no cultivo da uva 'BRS Clara'.

  11. Presencia de Stegomastodon (Gomphotheriidae, Proboscidea en el Pleistoceno Superior de la zona costera de Santa Clara del Mar (Argentina

    Directory of Open Access Journals (Sweden)

    Alberdi, M. T.

    2008-12-01

    Full Text Available Gomphotheriidae fossil remains from shore level of Santa Clara del Mar (Mar del Plata, Argentina are described. The comparative morphological and multivariate analysis of the m3 with other South American gomphotheres remains, mainly from Argentina, Bolivia and Brazil, allow their identification as Stegomastodon platensis. This species seems better adapted to live on the middle latitudes and warm climatic conditions. The possible movement routes from north to the most austral zones or areas are discussed. Stegomastodon from South America is a big-size form which is present along the East route and in several shore areas of Ecuador and Peru. In the present work some palaeoecological and palaeogeographical considerations are included. From a radiometric AMS datum, the remains of Santa Clara del Mar are dated in the late Pleistocene (17,880 ± 60 AP.Se describen restos bien conservados de gonfoterios (Mammalia, Proboscidea encontrados en los niveles pleistocenos que afloran en los acantilados costeros de Santa Clara del Mar (Mar del Plata, Argentina. Se analizan algunos de sus caracteres más importantes y se compara con otros gonfoterios provenientes de distintas localidades de Argentina, Brasil y Bolivia. El estudio comparativo, mediante análisis multivariante del m3, permitió su identificación como Stegomastodon platensis. Nosotros consideramos que todos los Gomphotheriidae del territorio actual de Argentina deberían incluirse en un único género y una única especie: Stegomastodon platensis. Esta especie parece estar mejor adaptada a vivir en latitudes medias, y en condiciones climáticas templadas. Se discuten sus posibles vías de desplazamiento desde el norte hacia zonas o áreas más australes. Stegomastodon de América del Sur es una forma de gran tamaño, que se encuentra a lo largo de la ruta del este y en algunas áreas costeras de Ecuador y Perú. En el presente artículo se incluyen algunas consideraciones paleoecológicas y

  12. FORMACIÓN DE LA CULTURA TURÍSTICA EN LAS COMUNIDADES ANFITRIONAS: BARRIO CENTRO, SANTA CLARA, CUBA.

    OpenAIRE

    Ramón González Herrera, Manuel; Castro Acevedo, Georgina

    2015-01-01

    El turismo cultural deberá estar concebido desde una perspectiva educativa con el fin de facilitar una actuación responsable. Es propósito de la investigación diseñar e implementar una estrategia participativa para la formación de la cultura turística en el Barrio Centro de Santa Clara, con el fin de promover el turismo cultural comunitario desde una perspectiva educativa. Se utiliza el enfoque metodológico cualitativo basado en la percepción y apreciación de la población con respecto al mode...

  13. Liquefaction Hazard Maps for Three Earthquake Scenarios for the Communities of San Jose, Campbell, Cupertino, Los Altos, Los Gatos, Milpitas, Mountain View, Palo Alto, Santa Clara, Saratoga, and Sunnyvale, Northern Santa Clara County, California

    Science.gov (United States)

    Holzer, Thomas L.; Noce, Thomas E.; Bennett, Michael J.

    2008-01-01

    Maps showing the probability of surface manifestations of liquefaction in the northern Santa Clara Valley were prepared with liquefaction probability curves. The area includes the communities of San Jose, Campbell, Cupertino, Los Altos, Los Gatos Milpitas, Mountain View, Palo Alto, Santa Clara, Saratoga, and Sunnyvale. The probability curves were based on complementary cumulative frequency distributions of the liquefaction potential index (LPI) for surficial geologic units in the study area. LPI values were computed with extensive cone penetration test soundings. Maps were developed for three earthquake scenarios, an M7.8 on the San Andreas Fault comparable to the 1906 event, an M6.7 on the Hayward Fault comparable to the 1868 event, and an M6.9 on the Calaveras Fault. Ground motions were estimated with the Boore and Atkinson (2008) attenuation relation. Liquefaction is predicted for all three events in young Holocene levee deposits along the major creeks. Liquefaction probabilities are highest for the M7.8 earthquake, ranging from 0.33 to 0.37 if a 1.5-m deep water table is assumed, and 0.10 to 0.14 if a 5-m deep water table is assumed. Liquefaction probabilities of the other surficial geologic units are less than 0.05. Probabilities for the scenario earthquakes are generally consistent with observations during historical earthquakes.

  14. Características físico-químicas e produtivas da videira 'BRS Clara' sob cultivo protegido Physical-chemical and productive characteristics of 'BRS Clara' under protected cultivation

    Directory of Open Access Journals (Sweden)

    Larissa Abgariani Colombo

    2011-09-01

    Full Text Available Atualmente, a preferência por uvas sem sementes vem aumentando nos mercados interno e externo, sendo uma alternativa a produção de uvas sob cultivo protegido. No entanto, o custo de produção de uvas finas para mesa tem sido afetado pela intensa necessidade de controle de doenças fúngicas, como o míldio (Plasmopara viticola. O trabalho teve como objetivo avaliar a produção e as características físicoquímicas dos frutos da videira 'BRS Clara' sobre os porta-enxertos 'IAC 572 Jales' e 'IAC 766 Campinas', sob diferentes tipos de cultivo protegido. O experimento foi realizado no município de Marialva-PR, durante duas safras regulares (set.-dez.2007, ago-dez.2008. O delineamento experimental foi o de blocos ao acaso, com quatro repetições, em arranjo fatorial 7 x 2 (sete tipos de cultivo protegido e dois porta-enxertos. Os tratamentos foram os seguintes tipos de cultivo: a. tela plástica sem fungicidas para míldio; b. tela plástica com fungicidas para míldio (padrão de controle da região; c. cobertura plástica sem fungicidas para míldio; d. cobertura plástica e 50% de redução do padrão de fungicidas para míldio; e. cobertura plástica e 75% de redução do padrão de fungicidas para míldio; f. cobertura plástica com fosfito e cobre, e g. cobertura plástica sem fungicidas. Verificou-se que o cultivo protegido não alterou as características produtivas da videira 'BRS Clara', como número de cachos e produção por planta; os porta-enxertos 'IAC 766' e 'IAC 572' são indicados para a produção da uva 'BRS Clara', e a utilização da cobertura plástica permite a redução do número de aplicações de fungicidas para míldio no cultivo da uva 'BRS Clara'.Currently the preference for seedless grapes is increasing in domestic and foreign markets and an alternative is to produce grapes under plastic cover. However, the increased costs of production of table grapes has been affected by the intense need to control fungal

  15. First record of a new epibionts suctorian ciliate Tokophrya huangmeiensis sp.n. (Ciliophora, Phyllopharyngea) from redclaw crayfish Cherax quadricarinatus von Martens 1868.

    Science.gov (United States)

    Tahir, Urfa Bin; Deng, Qiong; Li, Sen; Liu, Yang; Wang, Zhe; Gu, Zemao

    2017-05-22

    A new suctorian ciliate, Tokophrya huangmeiensis sp. n. was isolated from the carapace of redclaw crayfish Cherax quadricarinatus, during investigation of fish ciliates diversity in freshwater aquaculture of Hubei, China. The zooids of newly isolated ciliate were found aggregated into colonies on stalks of another ciliate Epistylis. The adults of this newly found species were characterized by an elongated pyramidal shaped and corrugated cell body with two fascicles of tentacles on the apical part of cell body. Stalks were hollow and transparent with longitudinal striations. The single contractile vacuole was located in the apical region and an elongated ρ-shaped macronucleus was situated along the long axis of cell body. Phylogenetic analyses showed that the subclass Suctoria is monophyletic and comprises the groups Exogenina, Evaginogenina and Endogenina, where Exogenina is the earliest branching lineage among these three groups. Tokophrya huangmeiensis sp. n. clustered with the other Tokophrya species and within the Endogenina subgroup as expected.

  16. 70 anos da guerreira: a mestiçagem brasileira na tradução musical de Clara Nunes

    Directory of Open Access Journals (Sweden)

    Expedito Leandro Silva

    2014-01-01

    Full Text Available Clara Francisca Gonçalves Pinheiro. Stage Name: Clara Nunes. On August 12, 2012 this vigorous woman from Paraopeba in the state of Minas Gerais would have turned 70 years old. Her family life, in the context of culture and religion, contributed to her musical de- velopment from an early age broadening her contact with folk music. In this sense, her songs started dialoging with the world of samba and with the universe of African-Brazilian religions. Among the songs that best represent the artist portrayal are “Canto das Três Raças” and “Brasil Mestiço Santuário da Fé”, sang by her in the 1980s, the lyrics and interpretation showed the social Brazilian ethnological context in great detail. However, the cries of the people and the musical manifestation reproduced in the songs present an ideology of nationhood whose identity is the Brazilian miscegenation which means the cultures of the native Indians, the white and the black people.

  17. De Palacio a Monasterio. Génesis y transformación del Real Monasterio de Santa Clara de Sevilla

    Directory of Open Access Journals (Sweden)

    Oliva Muñoz, Pablo

    2011-12-01

    Full Text Available The convent of Santa Clara is one of the largest religious buildings in Seville. Founded in 1289, it became the oldest second one in the city after that of San Clemente, which is located in the same northwestern area of the old Almohad wall. The comprehensive study of the building, as part of the rehabilitation project, has allowed knowing its evolution and the survival of structures previous to the granting of the land where it is sited.El convento de Santa Clara es uno de los edificios de carácter religioso más grandes de Sevilla. Fundado en 1289 se convierte en el segundo más antiguo de la ciudad tan sólo precedido por el de San Clemente que se ubica en el mismo sector del extremo Noroeste de la antigua cerca almohade. El estudio integral del edificio como parte del proyecto de rehabilitación nos ha permitido conocer su evolución y la pervivencia de estructuras heredadas previas a la concesión de los terrenos que ahora ocupa.

  18. Escribir para resistir la Soledad: Nostalgia, confesión y amor en cartas a Clara de Juan Rulfo

    Directory of Open Access Journals (Sweden)

    Carlos Julio Ayram Chede

    2013-01-01

    Full Text Available El presente artículo indaga en el conjunto de cartas que el escritor mexicano Juan Rulfo le escribe a Clara Aparicio entre los años 1944 y 1950 las cuales no solo representan un hecho poé- tico en sí mismo, sino que develan al menos tres estados que se conjugan y coexisten en el plano de la expresión de cada misiva: la nostalgia, la confesión y el amor. Por tanto, cada una de las cartas que escribe Rulfo le permite asegurar un lugar en el mundo que empieza a habitar desde la lejanía de su hogar y que comparte de manera permanente con Clara. Juan Rulfo se sustrae de la lógica de la cotidianidad para evaporar la rutinización de su vida en la escritura, podría afirmarse, que como San Juan de la Cruz o Gustavo Adolfo Bécquer, Rulfo se evade en la escritura como oportunidad para albergar un mundo incondicional donde existe él y su corazón, donde existe el sujeto que ama.

  19. The Quaternary Silver Creek Fault Beneath the Santa Clara Valley, California

    Science.gov (United States)

    Wentworth, Carl M.; Williams, Robert A.; Jachens, Robert C.; Graymer, Russell W.; Stephenson, William J.

    2010-01-01

    The northwest-trending Silver Creek Fault is a 40-km-long strike-slip fault in the eastern Santa Clara Valley, California, that has exhibited different behaviors within a changing San Andreas Fault system over the past 10-15 Ma. Quaternary alluvium several hundred meters thick that buries the northern half of the Silver Creek Fault, and that has been sampled by drilling and imaged in a detailed seismic reflection profile, provides a record of the Quaternary history of the fault. We assemble evidence from areal geology, stratigraphy, paleomagnetics, ground-water hydrology, potential-field geophysics, and reflection and earthquake seismology to determine the long history of the fault in order to evaluate its current behavior. The fault formed in the Miocene more than 100 km to the southeast, as the southwestern fault in a 5-km-wide right step to the Hayward Fault, within which the 40-km-long Evergreen pull-apart basin formed. Later, this basin was obliquely cut by the newly recognized Mt. Misery Fault to form a more direct connection to the Hayward Fault, although continued growth of the basin was sufficient to accommodate at least some late Pliocene alluvium. Large offset along the San Andreas-Calaveras-Mt Misery-Hayward Faults carried the basin northwestward almost to its present position when, about 2 Ma, the fault system was reorganized. This led to near abandonment of the faults bounding the pull-apart basin in favor of right slip extending the Calaveras Fault farther north before stepping west to the Hayward Fault, as it does today. Despite these changes, the Silver Creek Fault experienced a further 200 m of dip slip in the early Quaternary, from which we infer an associated 1.6 km or so of right slip, based on the ratio of the 40-km length of the strike-slip fault to a 5-km depth of the Evergreen Basin. This dip slip ends at a mid-Quaternary unconformity, above which the upper 300 m of alluvial cover exhibits a structural sag at the fault that we interpret as

  20. Role of essential lipids in copepod nutrition: no evidence for trophic upgrading of food quality by a marine ciliate

    DEFF Research Database (Denmark)

    Klein Breteler, W.; Koski, Marja; Rampen, S.

    2004-01-01

    copepod species consumed S. sulcatum at significant rates, but after 3 d, weight-specific ingestion decreased more than 2-fold. Larvae and copepodites feeding on S. sulcatum developed at suboptimal rates, survived poorly and showed abnormal morphology in comparison to control individuals fed a good-quality......The ciliate Strombidium sulcatum was used to feed and grow young stages of the copepods Temora longicornis (Muller) and Pseudocalanus elongatus (Boeck). The ciliate was cultured in the laboratory using either bacteria or the green alga Dunaliella sp. as a food source. Young copepodites of both...... Rhodomonas sp. diet. The specific mass of fatty acids in S. sulcatum was much lower than in the Dunaliella sp. diet, However, the fatty acid composition of the protozoan more or less resembled that of the food, lacking long-chain highly unsaturated fatty acids (HUFAs). Sterols only occurred in Dunaliella sp...

  1. Blooms of a benthic ciliate, Maristentor dinoferus (Heterotrichea: Maristentoridae, on coral reefs of Guam, Mariana Islands. (Note

    Directory of Open Access Journals (Sweden)

    Schefter, M.

    2012-05-01

    Full Text Available Maristentor dinoferus is visible in situ with the naked eye; it forms aggregations at several scales: individuals form dynamic clusters within colonies that are normally widely scattered, but in recurrent blooms during the dry seasons of 2005 through 2007, colonies often formed close together in patches (metapopulations. Patches were extremely abundant in Guam, both in Apra Harbor and on the outer coast. This is the first time that long-term changes in a benthic ciliate population have been documented in situ and that multi-scale patchiness has been reported for a benthic ciliate. Colonies were mapped and monitored; some patches over 1 m diameter were recorded. Since 2008, the distribution has again consisted of scattered colonies, as it did from 1999–2004.

  2. The effects of the ciliate Paramecium cf. caudatum Ehrenberg on toxin producing Cylindrospermopsis isolated from the Fitzroy River, Australia.

    Science.gov (United States)

    Fabbro, L; Baker, M; Dilvenvoorden, L; Pegg, G; Shiel, R

    2001-01-01

    The large ciliate Paramecium cf. caudatum Ehrenberg was found to be a successful grazer of toxin producing Cylindrospermopsis in the laboratory. The feeding rate increased with increasing cell concentration to 1367 cell animal hr-1 at 4.1 x 10(5) cells mL-1 but declined slightly at cell concentrations greater than this. Preliminary studies on the effects of this grazing on toxin concentrations in cultures of both straight and coiled forms of Cylindrospermopsis resulted in the production of different amounts of the toxin cylindrospermopsin in the different isolates. Differences in toxin production were also found between cultured and field populations from the lower Fitzroy River indicating that toxin production may be influenced by a suite of genetic and environmental factors. The proven ability of this ciliate to graze toxic Cylindrospermopsis provides some insight into interactions that may be able to control some toxic blooms in semiarid Australian conditions.

  3. Application Of Geowall Technology To The Analysis Of A Three Dimensional Geologic Map Of The Santa Clara (Silicon) Valley, California

    Science.gov (United States)

    Phelps, G. A.; Jachens, R. C.; Wentworth, C. M.; Langenheim, V. E.; Hanson, R. T.; Faunt, C. C.

    2003-12-01

    Geowall, a stereo projection system suitable for meetings and conferences, is being used to visualize, understand, interpret, and test a three-dimensional geologic map of the Santa Clara (Silicon) Valley, southern San Francisco Bay area, California. Geowall*, developed at the Electronic Visualization Laboratory associated with the University of Illinois, uses dual polarized images projected onto a polarization-preserving screen to create the illusion of three dimensions when viewed through polarized glasses. The map of Santa Clara Valley encompasses a 45 by 45 km area, extends to a depth of 14 km, and includes the northern Santa Clara Valley and surrounding hillsides between the active Calaveras and San Andreas faults. It is currently divided by several major faults into tectonic blocks, within which 9 units represent the Cenozoic and Mesozoic sections. Many of these units will be subdivided as the map evolves. The map is being constructed in EarthVision*(TM, Dynamic Graphics, Inc.), a geologic modeling software that includes three dimensional rendering and model manipulation capabilities. Earthvision generates data and model images of which the entire, or only portions of the model, can be viewed in three dimensions. The geowall presentation will explore the datasets and three-dimensional geologic map of Santa Clara Valley and structures defined by geologic mapping, stratigraphy, hydrology, potential field geophysics, seismic reflection, and earthquake seismicity. The map is the result of a collaborative effort among several earth science disciplines, and as such requires the integration of diverse datasets and the communication of diverse ideas. The geowall is particularly effective at promoting group discussion and analysis of the three-dimensional map, because the map can be displayed in a group setting as a 6x6 ft., stereo image. The spatial relationships of the datasets are easily seen, and the map can be deconstructed and particular relationships isolated

  4. Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans?under low?temperature

    OpenAIRE

    Yin, Fei; Sun, Peng; Wang, Jiteng; Gao, Quanxin

    2016-01-01

    Background Cryptocaryon irritans, a species of obligatory ciliate ectoparasite, can infect various species of marine teleost fish. Cryptocaryon irritans that fall to the seabed or aquarium bottom in winter can form ?dormant tomonts? and wake up when the temperature rises the next year. Abundant studies and analyses on the dormant tomonts were carried out at the transcriptome level, in order to investigate the molecular mechanism of C. irritans tomonts entering the dormant state under low-temp...

  5. Can the halophilic ciliate Fabrea salina be used as a bio-control of microalgae blooms in solar salterns?

    Science.gov (United States)

    Hong, Hyun Pyo; Choi, Joong Ki

    2015-09-01

    The microlage Dunaliella salina, a major producer in salterns, is a serious problem for salt production. In this study we tried to assess if Fabrea salina can control D. salina. By parameterising numerical and functional response (growth and grazing vs prey abundance, respectively) at 90 psu and 30°C, where the ciliate is abundant and grows well, we developed a predator-prey model. The model is used to explore how change in microalga growth rate affect the dynamics, and the functional response is used in combination with field data to assess the potential impact of F. salina on D. salina. Over the 20 d simulation the ciliate controlled the prey population under all prey growth rates; although once D. salina were exhausted below the threshold level, F. salina died due to starvation, allowing the alga to increase in abundance, resulting in one or two predatorprey cycle, depending on prey growth rate. In general, the model predicted trends observed by others in the field, suggesting that it provided a good prediction of what may occur under the conditions we examined. Likewise we show that the ciliate can have a high impact on microalgal populations in the field. Finally, a literature review indicated that F. salina could be a good competitor with other protozoa and metazoan in salterns, depending on salinity and temperature, which requires further study and attention. In summary, we encourage continued studies on this unique ciliate on solar salterns and suggest that it may be useful in the bio-control of micoalgae.

  6. Determination of trace elements in freshwater rotifers and ciliates by total reflection X-ray fluorescence spectrometry

    Science.gov (United States)

    Woelfl, S.; Óvári, M.; Nimptsch, J.; Neu, T. R.; Mages, M.

    2016-02-01

    Element determination in plankton is important for the assessment of metal contamination of aquatic environments. Until recently, it has been difficult to determine elemental content in rotifers or ciliates derived from natural plankton samples because of the difficulty in handling and separation of these fragile organisms. The aim of this study was to evaluate methods for separation of rotifers and large ciliates from natural plankton samples (μg range dry weight) and subsequent analysis of their elemental content using total-reflection X-ray fluorescence spectrometry (TXRF). Plankton samples were collected from different aquatic environments (three lakes, one river) in Chile, Argentina and Hungary. From one to eighty specimens of five rotifer species (Brachionus calyciflorus, Brachionus falcatus, Asplanchna sieboldii, Asplanchna sp., Philodina sp.) and four to twelve specimens of one large ciliate (Stentor amethystinus) were prepared according to the dry method originally developed for microcrustaceans, and analysed by TRXF following in situ microdigestion. Our results demonstrated that it possible to process these small and fragile organisms (individual dry mass: 0.17-9.39 μg ind- 1) via careful washing and preparation procedures. We found species-dependent differences of the element mass fractions for some of the elements studied (Cr, Mn, Fe, Ni, Cu, Zn, As, Pb), especially for Cu, Fe and Mn. One large rotifer species (A. sieboldii) also showed a negative correlation between individual dry weight and the element content for Pb, Ni and Cr. We conclude that our application of the in situ microdigestion-TRXF method is suitable even for rotifers and ciliates, greatly expanding the possibilities for use of plankton in biomonitoring of metal contamination in aquatic environments.

  7. Several RNase T2 enzymes function in induced tRNA and rRNA turnover in the ciliate Tetrahymena

    DEFF Research Database (Denmark)

    Andersen, Kasper Langebjerg; Collins, Kathleen

    2012-01-01

    RNase T2 enzymes are produced by a wide range of organisms and have been implicated to function in diverse cellular processes, including stress-induced anticodon loop cleavage of mature tRNAs to generate tRNA halves. Here we describe a family of eight RNase T2 genes (RNT2A-RNT2H) in the ciliate...... by growth arrest that functions to recycle idle protein synthesis machinery....

  8. The influence of environmental characteristics on the distribution of ciliates (Protozoa, Ciliophora in an urban stream of southeast Brazil

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    RJP. Dias

    Full Text Available The aim of this research was to study the ciliated protozoa community at three sampling stations that receive different levels of domestic sewage along the São Pedro Stream in the municipality of Juiz de Fora, Minas Gerais, Brazil, in order to determine the influence of organic pollution on this community and to assess the feasibility of using ciliates as water quality indicators. Four physical-chemical parameters of the water samples were evaluated: dissolved oxygen concentration, electrical conductivity, pH and temperature. The sediment was obtained manually, using dredges with capacity of 300 mL, at each collection point. Point 1 was located in a rural region that receives a low sewage load, while Points 2 and 3 were located in populated regions receiving high sewage loads. We found 22 ciliate species, of which 18 are included in the saprobic system and are considered bioindicators. These showed beta-mesosaprobic environments at Point 1 and alfa-mesosaprobic to polisaprobic environments at Points 2 and 3. The low levels of dissolved oxygen and the high electrical conductivity values at Points 2 and 3, together with the strong similarity between the ciliate taxocenoses of these points and the weak similarity between Point 1 and the other two, confirm the high sewage loads received at the latter two points. The combination of the biological indicators and physical-chemical analyses therefore proved itself to be an efficient method of evaluating water quality, and has excellent potential to support decisions on the conservation of headwaters and recuperation of degraded environments in lotic systems.

  9. Status and understanding of groundwater quality in the Santa Clara River Valley, 2007-California GAMA Priority Basin Project

    Science.gov (United States)

    Burton, Carmen A.; Montrella, Joseph; Landon, Matthew K.; Belitz, Kenneth

    2011-01-01

    Groundwater quality in the approximately 460-square-mile Santa Clara River Valley study unit was investigated from April through June 2007 as part of the Priority Basin Project of the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The GAMA Priority Basin Project is conducted by the U.S. Geological Survey (USGS) in collaboration with the California State Water Resources Control Board and the Lawrence Livermore National Laboratory. The Santa Clara River Valley study unit contains eight groundwater basins located in Ventura and Los Angeles Counties and is within the Transverse and Selected Peninsular Ranges hydrogeologic province. The Santa Clara River Valley study unit was designed to provide a spatially unbiased assessment of the quality of untreated (raw) groundwater in the primary aquifer system. The assessment is based on water-quality and ancillary data collected in 2007 by the USGS from 42 wells on a spatially distributed grid, and on water-quality data from the California Department of Public Health (CDPH) database. The primary aquifer system was defined as that part of the aquifer system corresponding to the perforation intervals of wells listed in the CDPH database for the Santa Clara River Valley study unit. The quality of groundwater in the primary aquifer system may differ from that in shallow or deep water-bearing zones; for example, shallow groundwater may be more vulnerable to surficial contamination. Eleven additional wells were sampled by the USGS to improve understanding of factors affecting water quality.The status assessment of the quality of the groundwater used data from samples analyzed for anthropogenic constituents, such as volatile organic compounds (VOCs) and pesticides, as well as naturally occurring inorganic constituents, such as major ions and trace elements. The status assessment is intended to characterize the quality of untreated groundwater resources in the primary aquifers of the Santa Clara River Valley study unit

  10. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote.

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    Jonathan A Eisen

    2006-09-01

    Full Text Available The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC, which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Overall, the gene set is robust, with more than 27,000 predicted protein-coding genes, 15,000 of which have strong matches to genes in other organisms. The functional diversity encoded by these genes is substantial and reflects the complexity of processes required for a free-living, predatory, single-celled organism. This is highlighted by the abundance of lineage-specific duplications of genes with predicted roles in sensing and responding to environmental conditions (e.g., kinases, using diverse resources (e.g., proteases and transporters, and generating structural complexity (e.g., kinesins and dyneins. In contrast to the other lineages of alveolates (apicomplexans and dinoflagellates, no compelling evidence could be found for plastid-derived genes in the genome. UGA, the only T. thermophila stop codon, is used in some genes to encode selenocysteine, thus making this organism the first known with the potential to translate all 64 codons in nuclear genes into amino acids. We present genomic evidence supporting the hypothesis that the excision of DNA from the MIC to generate the MAC specifically targets foreign DNA as a form of genome self-defense. The combination of the genome sequence, the functional diversity encoded therein, and the presence of some pathways missing from

  11. [Mating types in the ciliate Dileptus anser. Inheritance and genetic determination].

    Science.gov (United States)

    Iudin, A L; Uspenskaia, Z I

    2006-01-01

    Hybridological analysis of mating types (MTs) has been first made for the lower ciliate Dileptus anser. Clones of an initially unknown genotype belonging to three MTs (MT I, MT II and MT III), characteristic of D. anser, were isolated from natural reservoirs and further used for crosses. In one group crosses, synclonal inheritance and typical Mendelian behaviour of the character were observed over sexual generations of ciliates. The results suggest that MTs in D. anser may be directly controlled by a single mat locus with three alleles showing peck-order dominance (mat1 > mat2 > mat3). In other words, cells with mat1/mat1, mat1/mat2 and mat1/mat3 genotypes belong to MT I, those with mat2/mat2 and mat2/mat3, and the mat3/mat3 belong to MT II and MT III, respectively. Sexually mature exconjugant clones stably retain their MTs corresponding to their genotypes on vegetative reproduction. The progeny of other group crosses showed various deviations from typical Mendelian behaviour of the character. In some cases, standard Mendelian ratios were more or less violated. Most typical was instability of differentiation for MT in maturing exconjugant clones. Shortly after their maturation, the majority of clones change their MT, rather frequently more than once, although the finally established MT is stably inherited afterwards, during vegetative reproduction. When unstable, exconjugant clones can successively express two or even three MTs characteristic of this species, including MTs that should not have been expected on the basis of parental genotypes available in a given cross. It looks likely that the mat locus in D. anser is complex and multipotential; it is inherited as a whole providing for expression of any MT characteristic of the species (in this respect bearing similarity with Tetrahymena thermophila). Other mechanisms, epigenetic in particular (Nanney, 1958), determine the final expression of one of the three MT potentialities by a given exconjugant clone. Stable

  12. Generation of novel AAV variants by directed evolution for improved CFTR delivery to human ciliated airway epithelium.

    Science.gov (United States)

    Li, Wuping; Zhang, Liqun; Johnson, Jarrod S; Zhijian, Wu; Grieger, Joshua C; Ping-Jie, Xiao; Drouin, Lauren M; Agbandje-McKenna, Mavis; Pickles, Raymond J; Samulski, R Jude

    2009-12-01

    Recombinant adeno-associated virus (AAV) vectors expressing the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been used to deliver CFTR to the airway epithelium of cystic fibrosis (CF) patients. However, no significant CFTR function has been demonstrated likely due to low transduction efficiencies of the AAV vectors. To improve AAV transduction efficiency for human airway epithelium (HAE), we generated a chimeric AAV library and performed directed evolution of AAV on an in vitro model of human ciliated airway epithelium. Two independent and novel AAV variants were identified that contained capsid components from AAV-1, AAV-6, and/or AAV-9. The transduction efficiencies of the two novel AAV variants for human ciliated airway epithelium were three times higher than that for AAV-6. The novel variants were then used to deliver CFTR to ciliated airway epithelium from CF patients. Here we show that our novel AAV variants, but not the parental, AAV provide sufficient CFTR delivery to correct the chloride ion transport defect to ~25% levels measured in non-CF cells. These results suggest that directed evolution of AAV on relevant in vitro models will enable further improvements in CFTR gene transfer efficiency and the development of an efficacious and safe gene transfer vector for CF lung disease.

  13. Connecting alveolate cell biology with trophic ecology in the marine plankton using the ciliate Favella as a model.

    Science.gov (United States)

    Echevarria, Michael L; Wolfe, Gordon V; Strom, Suzanne L; Taylor, Alison R

    2014-10-01

    Planktonic alveolates (ciliates and dinoflagellates), key trophic links in marine planktonic communities, exhibit complex behaviors that are underappreciated by microbiologists and ecologists. Furthermore, the physiological mechanisms underlying these behaviors are still poorly understood except in a few freshwater model ciliates, which are significantly different in cell structure and behavior than marine planktonic species. Here, we argue for an interdisciplinary research approach to connect physiological mechanisms with population-level outcomes of behaviors. Presenting the tintinnid ciliate Favella as a model alveolate, we review its population ecology, behavior, and cellular/molecular biology in the context of sensory biology and synthesize past research and current findings to construct a conceptual model describing the sensory biology of Favella. We discuss how emerging genomic information and new technical methods for integrating research across different levels of biological organization are paving the way for rapid advance. These research approaches will yield a deeper understanding of the role that planktonic alveolates may play in biogeochemical cycles, and how they may respond to future ocean conditions. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  14. Acute toxicities of potassium permanganate, formalin, and Lugol's iodine solution to a marine ciliate, Pleuronema coronatum (ciliophora, scuticociliatida)

    Science.gov (United States)

    Yantao, Qiu; Weibo, Song

    2002-10-01

    Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC50 values) were given. Results of measurements indicated that 2 h-LC50 and 12 h-LC50 values of formalin on P. coronatum were 59.00×10-6 and 43.57×10-6, while those of Lugol’s solutions were 90.13 and 67.84×10-6 respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.

  15. Rumen ciliates of domestic cattle (Bos taurus taurus) in Kastamonu, Turkey, with the description of a new species.

    Science.gov (United States)

    Gürelli, Gözde

    2016-10-01

    Species composition and distribution of ciliates were investigated in the rumen contents of 25 domestic cattle (Bos taurus taurus L.) living in Kastamonu, Turkey. Forty-seven species and 37 morphotypes representing 15 genera were identified. Of them, a new species of Ostracodinium was recognized and described as Ostracodinium anatolicum n. sp. This new species has two caudal lobes. The dorsal lobe is small and rounded and the ventral lobe is triangular shaped and bent toward the dorsal side like a thick hook. Furthermore, the anterior end of the macronucleus (1/5 of the length) is bent toward the left like a hook. The density of rumen ciliates in cattle was 96.8±43.3×104cellsmL-1 and the mean number of ciliate species per host was 14.2±4.4. Entodinium longinucleatum, E. nanellum, E. simulans and Isotricha prostoma were the most abundant species, each with a prevalence of 88%. Entodinium chatterjeei, E. bifidum m. monospinosum, Hsiungia triciliata, Oligoisotricha bubali, Ostracodinium dogieli, O. mammosum and O. munham are new host records for cattle from Turkey. Copyright © 2016 Elsevier GmbH. All rights reserved.

  16. [Anatomical Vitamin C-Research during National Socialism and the Post-war Period: Max Clara's Human Experiments at the Munich Anatomical Institute].

    Science.gov (United States)

    Schûtz, Mathias; Schochow, Maximilian; Waschke, Jens; Marckmann, Georg; Steger, Florian

    2014-01-01

    In autumn of 1942, Max Clara (1899-1966) became chairman of the anatomical institute Munich. There, he intensified his research concerning the proof of vitamin C with the bodies of executed prisoners which were delivered by the Munich-Stadelheim prison. This research on human organs was pursued by applying ascorbic acid (Cebion) to prisoners before their execution. The paper investigates this intensified and radicalized anatomical research through human experiments, which Max Clara conducted in Munich and published from Istanbul during the postwar years, as well as its scientific references from the Nazi period.

  17. La Caja Real de la Villa de Santa Clara (Cuba: análisis histórico-contable (1689-1831

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    Yumaisi González Ochoa

    2017-07-01

    Full Text Available La Villa de Santa Clara en Cuba se fundó el 15 de julio de 1689, con una actividad económica fundamentalmente ganadera, se rigió en el orden administrativo y económico desde su fundación, al igual que las demás villas del país por las leyes u órdenes emitidas por la metrópoli española. Para el gobierno de las rentas reales, España utilizó las Cajas Reales como mecanismos de control y administración de los recursos en cada territorio americano bajo su dominio. La Caja Real de la Villa de Santa Clara se encontraba subordinada a la Caja Real Principal de la Habana, en la capital del país. A partir de la revisión de las Actas Capitulares del Cabildo de Santa Clara y los libros de cuentas conservados en el Archivo Nacional de Cuba, se explica el proceder de los administradores de los distintos ingresos y gastos de la Caja Real de la Villa de Santa Clara, así como la aplicación del método por cargo y data empleado para el registro de las operaciones y la interrelación entre los libros Manual, Mayor y de Caja del año 1831, y el uso de otros Libros Auxiliares.   Santa Clara village in Cuba was founded on July 15, 1689, with a primarily livestock economic activity, was governed at the administrative and economic order since its foundation, like other towns of the country by laws or orders issued by the Spanish metropolis. For the government in real incomes, Spain used the Cajas Reales as control mechanisms and management of resources in every American territory under its domain. The Caja Real of Santa Clara village was subordinated to the principal Caja Real in Havana, capital of the country. From the review of the Chapter Acts of the Cabildo of Santa Clara and accounting books preserved in the National Archives of Cuba, it's possible explain the proceeding of managers of the various revenue and expenditure of the Caja Real of Santa Clara village and the application of method of charge and discharge employed to register transactions

  18. The trophic role and impact of plankton ciliates in the microbial web structure of a tropical polymictic lake dominated by filamentous cyanobacteria

    Directory of Open Access Journals (Sweden)

    Alfonso Esquivel

    2016-03-01

    Full Text Available The recent interest in the plankton structures and dynamics in tropical and subtropical lakes has revealed important trends that set these lakes apart from temperate lakes, and one of the main differences is the enhanced importance of the microbial food web with respect to net plankton. Ciliates are a key component of subtropical and tropical microbial webs because of their role as dominant picoplankton grazers and their ability to channel picoplankton production to the uppermost trophic levels. Plankton ciliates have been found to play a crucial role in the survival of fish larvae in lakes that share several features with Lake Catemaco, a eutrophic tropical Mexican lake. Therefore, the plankton ciliate composition, abundance, and biomass of Lake Catemaco were studied to assess their role in the microbial food web. The data were obtained from surface and bottom water samples collected at eleven points during three surveys in 2011 and an additional survey in 2013, with the surveys covering the local climatic seasons. The most abundant components of the plankton ciliate assemblages were small prostomatids (Urotricha spp., choreotrichs (Rimostrombidium spp., cyclotrichs (Mesodinium and Askenasia, and scuticociliates (Cyclidium, Cinetochilum, Pleuronema, and Uronema. Other important ciliates in terms of abundance and/or biomass were haptorids (Actinobolina, Belonophrya, Monodinium, Paradileptus, and Laginophrya, Halteria, oligotrichs (Limnostrombidium and Pelagostrombidium, Linostomella, Bursaridium, Cyrtolophosis, and Litonotus. The ciliate abundance averaged 57 cells mL-1 and ranged from 14 to 113 cells mL-1. The mean ciliate biomass was 71 µg C L-1 and ranged from 10 to 202 µg C L-1. Differences were not detected in ciliate abundance or biomass between the sampling points or sampling depths (surface to bottom; however, significant differences were observed between seasons for both variables. Nano-sized filamentous cyanobacteria were the most

  19. Trichodina modesta: an exotic ciliate in the Neotropical region parasitizing an unusual host.

    Science.gov (United States)

    Valladão, Gustavo Moraes Ramos; Giannecchini, Luiz Gustavo; Martins, Maurício Laterça; de Pádua, Santiago Benites

    2015-01-01

    In this study, an important ornamental fish, Betta splendens (Osphronemidae), from three different Brazilian states was examined for parasitic infestations. Smears with parasites were impregnated with silver nitrate or stained using Giemsa for taxonomic evaluation. A disc-shaped trichodinid with a body diameter of 39.7 ± 3.3 µm, adhesive disc diameter of 32.9 ± 3.1 µm and denticulate ring diameter of 19.5 ± 2.0 µm was found. The morphological characteristics resembled those of Trichodina modesta Lom, 1970, a species that shows clear host specificity for Cypriniformes. Until now, its occurrence was restricted to the Eurasian region. In the present study, a new host for T. modesta is reported and therefore the first occurrence of this species in the Americas. The parasite was possibly introduced into the Neotropical region through the exotic fish trade, especially of Cypriniformes used by aquarists. The distribution of this ciliate is discussed and a checklist of localities and hosts for the species is provided.

  20. Trichodina modesta: an exotic ciliate in the Neotropical region parasitizing an unusual host

    Directory of Open Access Journals (Sweden)

    Gustavo Moraes Ramos Valladão

    Full Text Available In this study, an important ornamental fish, Betta splendens (Osphronemidae, from three different Brazilian states was examined for parasitic infestations. Smears with parasites were impregnated with silver nitrate or stained using Giemsa for taxonomic evaluation. A disc-shaped trichodinid with a body diameter of 39.7 ± 3.3 µm, adhesive disc diameter of 32.9 ± 3.1 µm and denticulate ring diameter of 19.5 ± 2.0 µm was found. The morphological characteristics resembled those of Trichodina modesta Lom, 1970, a species that shows clear host specificity for Cypriniformes. Until now, its occurrence was restricted to the Eurasian region. In the present study, a new host for T. modesta is reported and therefore the first occurrence of this species in the Americas. The parasite was possibly introduced into the Neotropical region through the exotic fish trade, especially of Cypriniformes used by aquarists. The distribution of this ciliate is discussed and a checklist of localities and hosts for the species is provided.

  1. A re-description of the ciliate genus and type species, Balantidium entozoon.

    Science.gov (United States)

    Grim, J Norman; Buonanno, Federico

    2009-08-01

    Members of the ciliate genus Balantidium possess a specialized "Villeneuve-Brachon's" field of somatic cilia to the right of the vestibule, or in a dextroral location. Specimens of the type species were collected in Italy and fixed for study by light microscopy, and scanning and transmission electron microscopy. The presence of the field in the type species and several other species of the genus indicates a need to re-describe the genus by including details of the ultrastructure of that field. Scanning electron microscopy shows that the field consists of one row of relatively short cilia of uniform length flanked on each side by 2-3 rows, or more, of very short cilia. Their kinetids have typical litostome structure in transmission electron micrographs. We speculate on a possible function for the Villeneuve-Brachon's field and also present morphometric data on the type species. The base sequence of the small subunit ribosomal RNA gene of Balantidium entozoon has been determined and found to differ by 5% from that of B. coli. Based on the location and ultrastructure, organelles found around the somatic kinetosomes and within inter-kinetal ridges of B. entozoon were identified as hydrogenosomes.

  2. Adaptation responses of individuals to environmental changes in the ciliate Euplotes crassus

    Science.gov (United States)

    Kim, Se-Joo; Kim, Jin-Hyoung; Ju, Se-Jong

    2017-03-01

    Although the response unit of living organisms to environmental changes is at the individual level, most experiments on the adaptation responses of ciliates have been conducted in batches, comprising multiple-individuals, due to their microscopic size. However, here, we confirmed that individuals undergo different division cycles in monocultures of Euplotes crassus. They also exhibited transcript variations of 4.63-fold in SSU and of 22.78- fold in Hsp70. Additionally, in salt-stressed E. crassus individuals, SSU transcripts of individuals varied by 6.92-fold at 27 psu, 8.69- fold at 32 psu, and 2.51-fold at 37 psu. However, the maximum difference in Hsp70 was only 4.23-fold under all conditions. These results suggest there may be different biological rhythms even in siblings derived from the same parent. It can also be inferred that various environmental factors have different effects on different E. crassus individuals. Therefore, to elucidate relationships between organism adaptations and environmental changes, studies at the individual level should be conducted with multi-individual approaches.

  3. In vitro culture technique for Cryptocaryon irritans, a parasitic ciliate of marine teleosts.

    Science.gov (United States)

    Yoshinaga, Tomoyoshi; Akiyama, Kousuke; Nishida, Sayoko; Nakane, Motoyuki; Ogawa, Kazuo; Hirose, Hitomi

    2007-12-13

    A medium for the in vitro culture of Cryptocaryon irritans, which is an obligatorily parasitic ciliate of marine teleosts and causes 'white spot disease', was developed. The medium consisted of a layer of cultured fish cells (FHM), with an agarose gel layer covering the cell layer. The agarose gel contained 0.22% agarose, 10% fetal calf serum, 100 I.U. ml(-1) Penicillin G potassium and 100 microg ml(-1) streptomycin sulphate. Theronts of C. irritans transformed to trophonts and grew to 180 microm in mean length in the medium, although they gradually decreased in number. When trophonts fully developed in medium were transferred into seawater 4 d after inoculation, approximately 70% of them transformed to encysted tomonts and released theronts. When fish were challenged with theronts obtained from in vitro-raised parasites, approximately 40% of the theronts were recovered from fish, indicating comparative infectivity of in vitro-raised theronts to those of in vivo-raised theronts. This is the first report that C. irritans fully developed in vitro and its entire life cycle was completed without a host fish.

  4. Ultrastructural features of the tomont of Cryptocaryon irritans (Ciliophora: Prostomatea), a parasitic ciliate of marine fishes.

    Science.gov (United States)

    Ma, Rui; Fan, Xinpeng; Yin, Fei; Ni, Bing; Gu, Fukang

    2017-05-01

    Numerous studies have been conducted on the cellular morphology of Cryptocaryon irritans. However, details regarding the tomont stage of its life cycle remain lacking. In this study, we investigated the morphology of the tomont stage throughout encystment and cell division using light and electron microscopy. Results showed that there was no secretion of encystation-specific secretory vesicles or extrusomes during formation of the cyst wall. Instead, the synthesis and construction of the C. irritans cyst wall materials may involve molecular events at the pellicle. The somatic cilia and the cytostome were present during encystment and covered by the newly formed cyst wall. New somatic cilia were continuously created between old cilia and showed various lengths during cell division, a process that was similar to morphogenesis in many free-living ciliates. During cell division inside the tomont, dividing daughter cells formed temporary cell chains with no oral primordia before separating from each other into dissociative tomite precursors. The process of cell division may not be accompanied by stomatogenesis, and new oral primordia in offspring cells likely formed before the dividing cell chains split into dissociative spherical tomites. Mitochondrial autophagy was observed in encysting C. irritans cells. Numerous endosymbionts and Golgi structures were observed in the tomont cytoplasm. Cellular metabolic activity in the C. irritans tomont was quite high, with large amounts of materials or cellular organelles potentially being synthesized and prepared for the following infective theront stage.

  5. Current status of parasitic ciliates Chilodonella spp. (Phyllopharyngea: Chilodonellidae) in freshwater fish aquaculture.

    Science.gov (United States)

    Bastos Gomes, G; Jerry, D R; Miller, T L; Hutson, K S

    2017-05-01

    Freshwater fish farming contributes to more than two-thirds of global aquaculture production. Parasitic ciliates are one of the largest causes of production loss in freshwater farmed fishes, with species from the genus Chilodonella being particularly problematic. While Chilodonella spp. include 'free-living' fauna, some species are involved in mortality events of fish, particularly in high-density aquaculture. Indeed, chilodonellosis causes major productivity losses in over 16 species of farmed freshwater fishes in more than 14 countries. Traditionally, Chilodonella species are identified based on morphological features; however, the genus comprises yet uncharacterized cryptic species, which indicates the necessity for molecular diagnostic methods. This review synthesizes current knowledge on the biology, ecology and geographic distribution of harmful Chilodonella spp. and examines pathological signs, diagnostic methods and treatments. Recent advances in molecular diagnostics and the ability to culture Chilodonella spp. in vitro will enable the development of preventative management practices and sustained freshwater fish aquaculture production. © 2016 John Wiley & Sons Ltd.

  6. Lithium-induced developmental anomalies in the spirotrich ciliate Stylonychia lemnae (Ciliophora, Hypotrichida).

    Science.gov (United States)

    Makhija, Seema; Gupta, Renu; Toteja, Ravi

    2015-08-01

    Lithium is known to have profound biological effects of varying intensity in different life forms. In the present investigation, the effect of lithium was studied on the spirotrich ciliate Stylonychia lemnae. Lithium treatment brings about quantitative changes in the patterning of ciliary structures in S. lemnae. The dorsal surface of the affected cells develops supernumerary ciliary kineties due to excessive proliferation of the kinetosomes. The ventral surface on the other hand develops fewer than normal cirri formed from reduced numbers of ciliary primordia. The adoral zone of membranelles (AZM) fails to remodel properly as, in certain segments, membranelles become disarranged and misaligned. Lithium-induced changes are transitory as the normal pattern is restored during recovery after the cells are shifted to normal medium, suggesting non-genic regulation of cortical pattern. Lithium also affects the process of cell proliferation as the number of cells undergoing division is negligible as compared to reorganizing cells. The results point to the extremely complex and heterogeneous organization of the cellular cortex (plasma membrane and cytoskeleton) which is capable of exerting autonomous control over the phenotype and cortical pattern. Copyright © 2015 Elsevier GmbH. All rights reserved.

  7. Focusing on genera to improve species identification: revised systematics of the ciliate Spirostomum.

    Science.gov (United States)

    Boscaro, Vittorio; Carducci, Daniela; Barbieri, Giovanna; Senra, Marcus V X; Andreoli, Ilaria; Erra, Fabrizio; Petroni, Giulio; Verni, Franco; Fokin, Sergei I

    2014-08-01

    Although many papers dealing with the description of new ciliate taxa are published each year, species taxonomy and identification in most groups of the phylum Ciliophora remain confused. This is largely due to a scarcity of surveys on the systematics of immediately higher levels (genera and families) providing data for old and new species together. Spirostomum is a common and distinctive inhabitant of fresh- and brackish water environments, including artificial and eutrophic ones, and is a good model for applied ecology and symbiosis research. Despite this, only 3 of the numerous species are commonly cited, and no studies have yet confirmed their monophyly, with the consequence that reproducibility of the results may be flawed. In this paper we present morphological and molecular data for 30 Spirostomum populations representing 6 different morphospecies, some of which were collected in previously unreported countries. We performed a detailed revision of Spirostomum systematics combining literature surveys, new data on hundreds of organisms and statistical and phylogenetic analyses; our results provide insights on the evolution, ecology and distribution of known morphospecies and a novel one: Spirostomum subtilis sp. n. We also offer tools for quick species identification. Copyright © 2014 Elsevier GmbH. All rights reserved.

  8. Propulsion of swimming microrobots inspired by metachronal waves in ciliates: from biology to material specifications.

    Science.gov (United States)

    Palagi, Stefano; Jager, Edwin W H; Mazzolai, Barbara; Beccai, Lucia

    2013-12-01

    The quest for swimming microrobots originates from possible applications in medicine, especially involving navigation in bodily fluids. Swimming microorganisms have become a source of inspiration because their propulsion mechanisms are effective in the low-Reynolds number regime. In this study, we address a propulsion mechanism inspired by metachronal waves, i.e. the spontaneous coordination of cilia leading to the fast swimming of ciliates. We analyse the biological mechanism (referring to its particular embodiment in Paramecium caudatum), and we investigate the contribution of its main features to the swimming performance, through a three-dimensional finite-elements model, in order to develop a simplified, yet effective artificial design. We propose a bioinspired propulsion mechanism for a swimming microrobot based on a continuous cylindrical electroactive surface exhibiting perpendicular wave deformations travelling longitudinally along its main axis. The simplified propulsion mechanism is conceived specifically for microrobots that embed a micro-actuation system capable of executing the bioinspired propulsion (self-propelled microrobots). Among the available electroactive polymers, we select polypyrrole as the possible actuation material and we assess it for this particular embodiment. The results are used to appoint target performance specifications for the development of improved or new electroactive materials to attain metachronal-waves-like propulsion.

  9. Space station image captures a red tide ciliate bloom at high spectral and spatial resolution.

    Science.gov (United States)

    Dierssen, Heidi; McManus, George B; Chlus, Adam; Qiu, Dajun; Gao, Bo-Cai; Lin, Senjie

    2015-12-01

    Mesodinium rubrum is a globally distributed nontoxic ciliate that is known to produce intense red-colored blooms using enslaved chloroplasts from its algal prey. Although frequent enough to have been observed by Darwin, blooms of M. rubrum are notoriously difficult to quantify because M. rubrum can aggregate into massive clouds of rusty-red water in a very short time due to its high growth rates and rapid swimming behavior and can disaggregate just as quickly by vertical or horizontal dispersion. A September 2012 hyperspectral image from the Hyperspectral Imager for the Coastal Ocean sensor aboard the International Space Station captured a dense red tide of M. rubrum (10(6) cells per liter) in surface waters of western Long Island Sound. Genetic data confirmed the identity of the chloroplast as a cryptophyte that was actively photosynthesizing. Microscopy indicated extremely high abundance of its yellow fluorescing signature pigment phycoerythrin. Spectral absorption and fluorescence features were related to ancillary photosynthetic pigments unique to this organism that cannot be observed with traditional satellites. Cell abundance was estimated at a resolution of 100 m using an algorithm based on the distinctive yellow fluorescence of phycoerythrin. Future development of hyperspectral satellites will allow for better enumeration of bloom-forming coastal plankton, the associated physical mechanisms, and contributions to marine productivity.

  10. Ultrastructure and molecular phylogeny of Mesodinium coatsi sp. nov., a benthic marine ciliate.

    Science.gov (United States)

    Nam, Seung Won; Shin, Woongghi; Kang, Misun; Yih, Wonho; Park, Myung Gil

    2015-01-01

    Mesodinium is a globally distributed ciliate genus forming frequent and recurrent blooms in diverse marine habitats. Here, we describe a new marine species, Mesodinium coatsi n. sp., originally isolated from interstitial water of surface sand samples collected at Mohang Beach, Korea. The species was maintained under a mixotrophic growth condition for longer than 1 yr by providing a cryptomonad, Chroomonas sp., as the sole prey. Cell morphology and subcellular structure were examined by light microscopy, scanning, and transmission electron microscopy, and molecular phylogeny was inferred from nuclear-encoded 18S rDNA sequence data. Like other Mesodinium species, M. coatsi consisted of two hemispheres separated by two types of kinetids, and had tentacles located at the oral end of the cell. Several food vacuoles were observed in the cytoplasm, and partially digested prey cells sometimes existed in food vacuoles. Kinetids and the associated accessory structures were quite similar to those previously reported, but M. coatsi was differentiated from other marine Mesodinium species by ultrastructural characters of the dikinetids, polykinetids, and tentacles. We also provided a detailed illustration of infraciliature. Molecular phylogeny revealed that M. coatsi and Mesodinium chamaeleon were closely related to each other. © 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.

  11. Cooperative social clusters are not destroyed by dispersal in a ciliate.

    Science.gov (United States)

    Schtickzelle, Nicolas; Fjerdingstad, Else J; Chaine, Alexis; Clobert, Jean

    2009-10-14

    The evolution of social cooperation is favored by aggregative behavior to facilitate stable social structure and proximity among kin. High dispersal rates reduce group stability and kin cohesion, so it is generally assumed that there is a fundamental trade-off between cooperation and dispersal. However, empirical tests of this relationship are rare. We tested this assumption experimentally using ten genetically isolated strains of a ciliate, Tetrahymena thermophila. The propensity for social aggregation was greater in strains with reduced cell quality and lower growth performance. While we found a trade-off between costly aggregation and local dispersal in phenotypic analyses, aggregative strains showed a dispersal polymorphism by producing either highly sedentary or long-distance dispersive cells, in contrast to less aggregative strains whose cells were monomorphic local dispersers. High dispersal among aggregative strains may not destroy group stability in T. thermophila because the dispersal polymorphism allows social strains to more readily escape kin groups than less aggregative strains, yet still benefit from stable group membership among sedentary morphs. Such dispersal polymorphisms should be common in other social organisms, serving to alter the nature of the negative impact of dispersal on social evolution.

  12. Cooperative social clusters are not destroyed by dispersal in a ciliate

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    Chaine Alexis

    2009-10-01

    Full Text Available Abstract Background The evolution of social cooperation is favored by aggregative behavior to facilitate stable social structure and proximity among kin. High dispersal rates reduce group stability and kin cohesion, so it is generally assumed that there is a fundamental trade-off between cooperation and dispersal. However, empirical tests of this relationship are rare. We tested this assumption experimentally using ten genetically isolated strains of a ciliate, Tetrahymena thermophila. Results The propensity for social aggregation was greater in strains with reduced cell quality and lower growth performance. While we found a trade-off between costly aggregation and local dispersal in phenotypic analyses, aggregative strains showed a dispersal polymorphism by producing either highly sedentary or long-distance dispersive cells, in contrast to less aggregative strains whose cells were monomorphic local dispersers. Conclusion High dispersal among aggregative strains may not destroy group stability in T. thermophila because the dispersal polymorphism allows social strains to more readily escape kin groups than less aggregative strains, yet still benefit from stable group membership among sedentary morphs. Such dispersal polymorphisms should be common in other social organisms, serving to alter the nature of the negative impact of dispersal on social evolution.

  13. Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation

    Directory of Open Access Journals (Sweden)

    Mariana Fonseca Rossi

    2013-11-01

    Full Text Available The objective of this study was to assess whether the straining of rumen content samples influences the estimation of protozoal density. Ninety rumen samples were obtained from 30 cattle (three samples per animal. The samples were subjected to one of three treatments at the moment of collection: 1 fixation informalin without straining(control treatment, 2 straining before fixation informalin, or 3straining after fixation informalin.To test the hypothesis of the variation in the protozoa composition in the samples, multivariate analyses with non-metric multidimensional scaling (NMDS were carried out. The diversity and density of rumen protozoa were negatively affected by straining before fixation.In the pre-filtered sample, the number of ciliates from the genus Entodinium was reduced, and no individuals from the Diploplastron, Elytroplastron and Eudiplodinium genera were detected; these effects were not observed in the other two treatments. Straining after fixation did not interfere with the diversity of the ruminal community, but the abundance of protozoa was greater thanin the control treatment and significantly greater than in the samples filtered before fixation. These factors suggest that post-fixation straining is the recommended technique to analyze rumen protozoa.

  14. Spatiotemporal sites of DNA replication in macro- and micronuclei of the ciliate Paramecium caudatum.

    Science.gov (United States)

    Tanaka, Tsubasa; Watanabe, Tsuyoshi

    2003-01-01

    Spatiotemporal sites of DNA replication in macro- and micronuclei of the ciliated protozoan Paramecium caudatum were analyzed by confocal laser scanning microscopy following incorporation of the thymidine analogue BrdU and indirect immunofluorescence. In the macronucleus, replication sites were localized to numerous small domains and scattered throughout the nucleoplasm. This pattern persisted during all periods of the S phase. A single constant pattern with discrete replication foci was also observed in the micronucleus. No obvious differences were seen between the two kinds of nuclei. Pulse-chase-pulse double-labeling experiments with two thymidine analogues (CldU and IdU) revealed that dispersed sites of replication were activated at different times during the S phase and a replication site takes about 2h to complete replication in the macronucleus. When cells were labeled by BrUTP to examine transcriptional activity in the two kinds of nuclei, incorporation of BrUTP into the macronucleus occurred throughout the cell cycle, whereas there was no detectable RNA synthesis in the micronucleus. From these findings, we conclude that, despite large differences in structure and function of macro- and micronuclear genomes, both nuclei show a similar replication pattern with discrete subnuclear foci scattered throughout the nucleoplasm at all times during the S phase.

  15. Distribution of a centrosomal antigen during morphogenesis in the ciliated protozoan Euplotes.

    Science.gov (United States)

    Fleury, A; Lemullois, M; Coffe, G

    1998-07-01

    Ciliates assemble basal bodies in great number at many stages of the life-cycle. In order to understand their assembly mechanisms, we screened a library of monoclonal antibodies directed against pericentriolar material. One of these antibodies, CTR210, was used previously to follow steps of this assembly process: in Paraurostyla, new basal bodies appear along a scaffold of linear structures recognized by this antibody. The very unusual behavior of this antigen deserved confirmation in other species. In the present study, we show by immunofluorescence that, in another phylogenetically very distant species, Euplotes, basal bodies are assembled in the same pathway during division. In addition, this antibody recognizes a filamentous ring located at the division furrow and linking many basal body assemblages. By cell fractionation and cytoskeletal extraction, we obtained fractions enriched in basal bodies and associated material. Such fractions still display a high complexity in protein composition. These fractions were used to characterize the main target of the antibody as a doublet of 45 kDa. These results confirm previous results in terms of functionality of the protein recognized by the antibody, but raise new questions in terms of the assignment of the recognized protein to the HSP70 family as hypothesized previously.

  16. C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication.

    Science.gov (United States)

    Wang, Juan; Silva, Malan; Haas, Leonard A; Morsci, Natalia S; Nguyen, Ken C Q; Hall, David H; Barr, Maureen M

    2014-03-03

    Cells release extracellular vesicles (ECVs) that play important roles in intercellular communication and may mediate a broad range of physiological and pathological processes. Many fundamental aspects of ECV biogenesis and signaling have yet to be determined, with ECV detection being a challenge and obstacle due to the small size (100 nm) of the ECVs. We developed an in vivo system to visualize the dynamic release of GFP-labeled ECVs. We show here that specific Caenorhabdidits elegans ciliated sensory neurons shed and release ECVs containing GFP-tagged polycystins LOV-1 and PKD-2. These ECVs are also abundant in the lumen surrounding the cilium. Electron tomography and genetic analysis indicate that ECV biogenesis occurs via budding from the plasma membrane at the ciliary base and not via fusion of multivesicular bodies. Intraflagellar transport and kinesin-3 KLP-6 are required for environmental release of PKD-2::GFP-containing ECVs. ECVs isolated from wild-type animals induce male tail-chasing behavior, while ECVs isolated from klp-6 animals and lacking PKD-2::GFP do not. We conclude that environmentally released ECVs play a role in animal communication and mating-related behaviors. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Studies on the in vitro cultivation of ciliate protozoa from the kangaroo forestomach.

    Science.gov (United States)

    Dehority, Burk A; Wright, André-Denis G

    2014-08-01

    The methods used for culturing rumen protozoa were found to be unsatisfactory for growth of ciliate protozoa from the kangaroo forestomach. Based on published measurements of physical parameters in the marsupial forestomach, several modifications were incorporated into the procedure, i.e., an increase in % hydrogen in the gas phase, adjustment of initial pH of the medium to 6.9-7.0 range, feed only forage as a substrate and incubate at a lower temperature (33-36 °C). Only incubation at the lower temperature increased survival time of the kangaroo protozoa. Two species of Bitricha were still viable after 28 d in culture. Cultures had to be terminated at that time. One of the species differed considerably in size and shape from previously described species and based on 18S rRNA data, may represent a new species of Bitricha. The second species, present in low numbers was identified as Bitricha oblata. In a separate trial, Macropodinium yalanbense survived for 11 d, at which time these cultures also had to be terminated. Copyright © 2014 Elsevier GmbH. All rights reserved.

  18. Estudio de foco de paragonimosis en Fuente Clara, Robledo, área periurbana de Medellín, Antioquia

    Directory of Open Access Journals (Sweden)

    Luz Elena Velásquez

    2008-09-01

    Full Text Available Introducción. La información sobre paragonimosis humana en Colombia llevó a suponer que los focos de la enfermedad estaban localizados en sectores selváticos; sin embargo, durante 2005 se hallaron cangrejos infectados con Paragonimus sp. en Fuente Clara, zona urbana de Medellín. Esto motivó la investigación. Objetivo. Realizar un estudio ecoepidemiológico de la paragonimosis con la participación de la comunidad para promover un manejo adecuado de los ecosistemas acuáticos. Materiales y métodos. Se realizó búsqueda de huéspedes silvestres y humanos de Paragonimus sp. en Fuente Clara. La presencia de formas larvarias y gusanos adultos del digéneo se evaluó en moluscos, crustáceos y mamíferos. En las personas voluntarias se hizo diagnóstico en esputo. Al agua de la quebrada La Puerta se le midieron: coliformes totales/ fecales, pH, conductividad y oxígeno disuelto. Con niños y adolescentes se realizaron talleres educativos utilizando técnicas lúdicas. Resultados. Los porcentajes de infección encontrados fueron: caracoles, 0,07%; cangrejos, 55,5%; mamíferos, 25%, y personas, 0%. Durante los talleres educativos se manifestó la importancia de los recursos naturales del barrio. Los niños identificaron los huéspedes de Paragonimus sp. y detectaron los factores de riesgo para adquirir la enfermedad. Se determinó que el agua de la quebrada La Puerta no es apta para el consumo y la recreación de las personas. Conclusiones. Se señala a Fuente Clara como el primer foco de paragonimosis en zona urbana de Colombia, donde la manipulación y el consumo de cangrejos ponen en riesgo de adquirir la infección a sus habitantes; se sugiere realizar vigilancia de la enfermedad en el sector.

  19. Inter-annual ciliate distribution variation within the late stratification oxycline in a monomictic lake, Lake Alchichica (Mexico

    Directory of Open Access Journals (Sweden)

    Ximena Sánchez Medina

    2016-03-01

    Full Text Available noxia, and ciliates might play a very important role in the plankton community budget there. We analysed changes in the composition and biomass of the ciliate assemblage and other microbial loop components throughout the oxycline just at the end of stratification in a warm-monomictic lake, Lake Alchichica, Mexico (four samplings: 2006-2008, 2010; the results were compared with those obtained from another lake from the region, La Preciosa, sampled in 2010. Bacteria, autotrophic picoplankton (APP and flagellates were analysed using epifluorescence microscopy. Ciliates were evaluated either in DAPI stained samples (looking for pigmented organelles and/or ingested phototrophs or in quantitative protargol stain (QPS permanent preparations, where they were identified at the genus or species level. The end of the stratification period in Lake Alchichica was characterized by almost uniform heterotrophic picoplankton (HPP numbers (106 cells mL-1 throughout the water column. Meanwhile, APP showed epilimnetic and/or metalimnetic maxima of 105 cells mL-1 followed by an order of magnitude drop in the hypolimnion. A very important peak (105 cells mL-1 of the autotrophic or mixotrophic flagellate Pyramimonas sp. was observed repeatedly above and within the oxycline of Lake Alchichica. Ciliate biomass maxima were found around the oxycline and in the above-bottom layer. The top of the oxycline was dominated by Euplotes spp. and Spirostomum teres fine- to coarse-filter feeders (feeding upon APP, nanodiatoms and algae. Raptorial haptorids (in particular, Phialina sp. were the second most important group, generally occupying the layer below euplotids, followed by Holophrya and Prorodon facultative anaerobic prostomes. Sometimes, strictly anaerobic Caenomorpha sp. was found to be important in the anoxic hypolimnion. Minute picoplankton feeding species (both APP and heterotrophic bacteria feeders were important throughout the water column: in the epilimnion

  20. Deformation from the 1989 Loma Prieta earthquake near the southwest margin of the Santa Clara Valley, California

    Science.gov (United States)

    Schmidt, Kevin M.; Ellen, Stephen D.; Peterson, David M.

    2014-01-01

    Damage to pavement and near-surface utility pipes, caused by the 17 October 1989, Loma Prieta earthquake, provides evidence for ground deformation in a 663 km2 area near the southwest margin of the Santa Clara Valley, California (USA). A total of 1427 damage sites, collected from more than 30 sources, are concentrated in four zones, three of which lie near previously mapped faults. In one of these zones, the channel lining of Los Gatos Creek, a 2-km-long concrete strip trending perpendicular to regional geologic structure, was broken by thrusts that were concentrated in two belts, each several tens of meters wide, separated by more than 300 m of relatively undeformed concrete.

  1. Neogene contraction between the San Andreas fault and the Santa Clara Valley, San Francisco Bay region, California

    Science.gov (United States)

    McLaughlin, R.J.; Langenheim, V.E.; Schmidt, K.M.; Jachens, R.C.; Stanley, R.G.; Jayko, A.S.; McDougall, K.A.; Tinsley, J.C.; Valin, Z.C.

    1999-01-01

    In the southern San Francisco Bay region of California, oblique dextral reverse faults that verge northeastward from the San Andreas fault experienced triggered slip during the 1989 M7.1 Loma Prieta earthquake. The role of these range-front thrusts in the evolution of the San Andreas fault system and the future seismic hazard that they may pose to the urban Santa Clara Valley are poorly understood. Based on recent geologic mapping and geophysical investigations, we propose that the range-front thrust system evolved in conjunction with development of the San Andreas fault system. In the early Miocene, the region was dominated by a system of northwestwardly propagating, basin-bounding, transtensional faults. Beginning as early as middle Miocene time, however, the transtensional faulting was superseded by transpressional NE-stepping thrust and reverse faults of the range-front thrust system. Age constraints on the thrust faults indicate that the locus of contraction has focused on the Monte Vista, Shannon, and Berrocal faults since about 4.8 Ma. Fault slip and fold reconstructions suggest that crustal shortening between the San Andreas fault and the Santa Clara Valley within this time frame is ~21%, amounting to as much as 3.2 km at a rate of 0.6 mm/yr. Rates probably have not remained constant; average rates appear to have been much lower in the past few 100 ka. The distribution of coseismic surface contraction during the Loma Prieta earthquake, active seismicity, late Pleistocene to Holocene fluvial terrace warping, and geodetic data further suggest that the active range-front thrust system includes blind thrusts. Critical unresolved issues include information on the near-surface locations of buried thrusts, the timing of recent thrust earthquake events, and their recurrence in relation to earthquakes on the San Andreas fault.

  2. Seroprevalencia de babesiosis bovina en la hacienda Vegas de la Clara, Gómez Plata (Antioquia, 2008

    Directory of Open Access Journals (Sweden)

    Richard Zapata Salas

    2011-06-01

    Full Text Available La babesiosis es una enfermedad del ganado bovino transmitida por la garrapata Rhipicephalus (Boophilus microplus y causada por los parásitos protozoarios Babesia bovis y B. bigemina. Una zona se considera epizootiológicamente estable frente a Babesia spp, cuando el 75% de los bovinos entre las edades de 3 a 9 meses son serorreactivos (IgG frente a Babesia bovis y Babesia bigemina y no hay evidencia de signos clínicos. El objetivo de esta investigación fue determinar la seroprevalencia de Babesia bovis y Babesia bigemina en el ganado bovino de la hacienda Vegas de la Clara (Universidad de Antioquia, Gómez Plata, Antioquia, por medio de inmunofluorescencia indirecta. Se diseñó un estudio descriptivo prospectivo con análisis de corte transversal. Fue evaluada toda la población bovina de la hacienda Vegas de la Clara (n = 118. Las muestras fueron evaluadas por inmunofluorescencia indirecta para la detección de anticuerpos tipo IgG específicos contra Babesia bovis y B. bigemina. La serorreactividad obtenida en los bovinos evaluados, para al menos una especie de Babesia fue del 89,8%, para Babesia bovis del 83,8%, mientras que para B. bigemina del 61%. Se obtuvo una relación estadísticamente significativa entre la serorreactividad para B. bigemina y la frecuencia del tratamiento garrapaticida. La serorreactividad indica estabilidad enzoótica en el hato para B. bovis, mientras que para B. bigemina se encontró que la frecuencia del tratamiento garrapaticida interrumpe su ciclo de transmisión.

  3. POR UMA EPISTEME DIALÓGICA, SENSÍVEL E CRIATIVA: UMA HOMENAGEM À ANA CLARA TORRES RIBEIRO

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    Anita Loureiro de Oliveira

    2012-09-01

    Full Text Available 1024x768 Normal 0 21 false false false PT-BR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Este artigo pretende ser uma homenagem à Professora Ana Clara Torres Ribeiro e ao mesmo tempo uma reunião de algumas das contribuições de seu pensamento para a consolidação de uma episteme dialógica, sensível e criativa, experimentada na pesquisa para a Tese Música e Vida Urbana (OLIVEIRA, 2008 e que em projetos acadêmicos e práticas pedagógicas e de pesquisa. Ana Clara tinha um olhar sensível para o outro. Este gesto não só era experimentado no cotidiano, como constituía parte significativa de sua proposta de teórico-metodológica.

  4. Caracterización de las farmacias comunitarias de Santa Clara para la implementación de la Dispensación de Medicamentos

    National Research Council Canada - National Science Library

    Liset Jaramillo Hernández; Abdel Bermúdez del Sol; Yelien Martín Fadragas; Jorge Luis Morales Martínez; Ydanelly San Gabino Paredes

    2012-01-01

    ...: caracterizar las farmacias comunitarias de Santa Clara para la implementación del Servicio de Dispensación de Medicamentos. Métodos: se realizó una investigación en sistemas y servicios de salud con un enfoque...

  5. Characterization of rumen ciliate community composition in domestic sheep, deer, and cattle, feeding on varying diets, by means of PCR-DGGE and clone libraries.

    Science.gov (United States)

    Kittelmann, Sandra; Janssen, Peter H

    2011-03-01

    The structure and variability of ciliate protozoal communities in the rumens of domestic New Zealand ruminants feeding on different diets was investigated. The relative abundance of ciliates compared with bacteria was similar across all samples. However, molecular fingerprinting of communities showed ruminant-specific differences in species composition. Community compositions of cattle were significantly influenced by diet. In contrast, diet effects in deer and sheep were weaker than the animal-to-animal variation. Cloning and sequencing of almost-full-length 18S rRNA genes from representative samples revealed that New Zealand ruminants were colonized by at least nine genera of ciliates and allowed the assignment of samples to two distinct community types. Cattle contained A-type communities, with most sequences closely related to those of the genera Polyplastron and Ostracodinium. Deer and sheep (with one exception) harboured B-type communities, with the majority of sequences belonging to the genera Epidinium and Eudiplodinium. It has been suggested that species composition of ciliate communities may impact methane formation in ruminants, with the B-type producing more methane. Therefore, manipulation of ciliate communities may be a means of mitigating methane emissions from grazing sheep and deer in New Zealand. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  6. Ciliate Nassula sp. grazing on a microcystin-producing cyanobacterium (Planktothrix agardhii): impact on cell growth and in the microcystin fractions.

    Science.gov (United States)

    Combes, Audrey; Dellinger, Marc; Cadel-six, Sabrina; Amand, Severine; Comte, Katia

    2013-01-15

    The proliferation of microcystins (MCs)-producing cyanobacteria (MCs) can have detrimental effects on the food chain in aquatic environments. Until recently, few studies had focused on the fate of MCs in exposed organisms, such as primary consumers of cyanobacteria. In this study, we investigate the impact of an MC-producing strain of the cyanobacterium Planktothrix agardhii on the growth and physiology of a Nassula sp. ciliate isolated from a non-toxic cyanobacterial bloom. We show that this Nassula sp. strain was able to consume and grow while feeding exclusively on an MC-producing cyanobacterium over a prolonged period of time (8 months). In short-term exposure experiments (8 days), ciliates consuming an MC-producing cyanobacterial strain displayed slower growth rate and higher levels of antioxidant enzymes than ciliates feeding on two non-MC-producing strains. Three high-performance methods (LC/MS, LC/MS-MS and ELISA) were used to quantify the free and bound MCs in the culture medium and in the cells. We show that ciliate grazing led to a marked decrease in free MCs (methanol extractable) in cells, the MCs were therefore no longer found in the surrounding culture medium. These findings suggest that MCs may have undergone redistribution (free vs bound MCs) or chemical degradation within the ciliates. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. Ciliate Nassula sp. grazing on a microcystin-producing cyanobacterium (Planktothrix agardhii): impact on cell growth and in the microcystin fractions

    Energy Technology Data Exchange (ETDEWEB)

    Combes, Audrey; Dellinger, Marc [' Molecules de communication et adaptation des microorganismes' , UMR 7245 CNRS-MNHN, Museum national d' Histoire naturelle, CP 39, 57 rue Cuvier, F-75231 Paris Cedex 05 (France); Cadel-six, Sabrina [' Unite Caracterisation des Toxines' - Laboratoire de securite des aliments de Maisons-Alfort - ANSES, F-94701 Maisons Alfort Cedex (France); Amand, Severine [' Molecules de communication et adaptation des microorganismes' , UMR 7245 CNRS-MNHN, Museum national d' Histoire naturelle, CP 39, 57 rue Cuvier, F-75231 Paris Cedex 05 (France); Comte, Katia, E-mail: kcomte@mnhn.fr [' Molecules de communication et adaptation des microorganismes' , UMR 7245 CNRS-MNHN, Museum national d' Histoire naturelle, CP 39, 57 rue Cuvier, F-75231 Paris Cedex 05 (France)

    2013-01-15

    The proliferation of microcystins (MCs)-producing cyanobacteria (MCs) can have detrimental effects on the food chain in aquatic environments. Until recently, few studies had focused on the fate of MCs in exposed organisms, such as primary consumers of cyanobacteria. In this study, we investigate the impact of an MC-producing strain of the cyanobacterium Planktothrix agardhii on the growth and physiology of a Nassula sp. ciliate isolated from a non-toxic cyanobacterial bloom. We show that this Nassula sp. strain was able to consume and grow while feeding exclusively on an MC-producing cyanobacterium over a prolonged period of time (8 months). In short-term exposure experiments (8 days), ciliates consuming an MC-producing cyanobacterial strain displayed slower growth rate and higher levels of antioxidant enzymes than ciliates feeding on two non-MC-producing strains. Three high-performance methods (LC/MS, LC/MS-MS and ELISA) were used to quantify the free and bound MCs in the culture medium and in the cells. We show that ciliate grazing led to a marked decrease in free MCs (methanol extractable) in cells, the MCs were therefore no longer found in the surrounding culture medium. These findings suggest that MCs may have undergone redistribution (free vs bound MCs) or chemical degradation within the ciliates.

  8. Further insights into the phylogeny of two ciliate classes Nassophorea and Prostomatea (Protista, Ciliophora).

    Science.gov (United States)

    Zhang, Qianqian; Yi, Zhenzhen; Fan, Xinpeng; Warren, Alan; Gong, Jun; Song, Weibo

    2014-01-01

    The Nassophorea and Prostomatea are two of the key classes in understanding the morphological diversification and higher classification of the phylum Ciliophora. However, their phylogenetic relationships with other ciliate groups within the subphylum Intramacronucleata remain elusive. In this study, we investigated the small and large subunit (SSU and LSU) rRNA gene-based phylogeny of these groups with sequences of additional taxa including several key species. The results show that: (1) the class Nassophorea remains polyphyletic, with the microthoracids clustering with the Phyllopharyngea, whereas the nassulids represent a basal group of the CONthreeP superclade in the SSU tree; (2) the Prostomatea is not depicted as a monophyletic group in phylogenetic trees, and the monophyly of this class is marginally rejected by statistical tree topology tests; (3) the nassulid genus Parafurgasonia is more closely related to the family Colpodidiidae than to Furgasonia; (4) Paranassula, which was previously thought to be a nassulid, is phylogenetically related to the oligohymenophorean peniculids in both the SSU and LSU trees; (5) the microthoracid genus Discotricha does not group with the other microthoracids in either SSU or LSU trees; (6) the family Plagiocampidae is closely related to the prostome parasite Cryptocaryon irritans and to the family Urotrichidae in the order Prorodontida; and (7) the family Placidae, represented by Placus salinus, is sister to the family Holophryidae in the order Prorodontida. Based on the present data, we consider the genus Discotricha to be an unclassified taxon within the CONthreeP. We also propose resurrecting the order Paranassulida and classifying it within the subclass Peniculia, class Oligohymenophorea. Primary and secondary structure signatures for higher taxa within Phyllopharyngea and Nassophorea are supplied. Copyright © 2013 Elsevier Inc. All rights reserved.

  9. Preliminary insights into the impact of dietary starch on the ciliate, Neobalantidium coli, in captive chimpanzees.

    Directory of Open Access Journals (Sweden)

    Kateřina Schovancová

    Full Text Available Infections caused by the intestinal ciliate Neobalantidium coli are asymptomatic in most hosts. In humans and captive African great apes clinical infections occasionally occur, manifested mainly by dysentery; however, factors responsible for development of clinical balantidiasis have not been fully clarified. We studied the effect of dietary starch on the intensities of infection by N. coli in two groups of captive chimpanzees. Adult chimpanzees infected by N. coli from the Hodonín Zoo and from the Brno Zoo, Czech Republic, were fed with a high starch diet (HSD (average 14.7% of starch for 14 days, followed by a five-day transition period and subsequently with a period of low starch diet (LoSD (average 0.1% of starch for another 14 days. We collected fecal samples during the last seven days of HSD and LoSD and fixed them in 10% formalin. We quantified trophozoites of N. coli using the FLOTAC method. The numbers of N. coli trophozoites were higher during the HSD (mean ± SD: 49.0 ± 134.7 than during the LoSD (3.5 ± 6.8. A generalized linear mixed-effects model revealed significantly lower numbers of the N. coli trophozoites in the feces during the LoSD period in comparison to the HSD period (treatment contrast LoSD vs. HSD: 2.7 ± 0.06 (SE, z = 47.7; p<<0.001. We conclude that our data provide a first indication that starch-rich diet might be responsible for high intensities of infection of N. coli in captive individuals and might predispose them for clinically manifested balantidiasis. We discuss the potential nutritional modifications to host diets that can be implemented in part to control N. coli infections.

  10. Small RNA-mediated regulation of DNA dosage in the ciliateOxytricha.

    Science.gov (United States)

    Khurana, Jaspreet S; Clay, Derek M; Moreira, Sandrine; Wang, Xing; Landweber, Laura F

    2018-01-01

    Dicer-dependent small noncoding RNAs play important roles in gene regulation in a wide variety of organisms. Endogenous small interfering RNAs (siRNAs) are part of an ancient pathway of transposon control in plants and animals. The ciliate, Oxytricha trifallax, has approximately 16,000 gene-sized chromosomes in its somatic nucleus. Long noncoding RNAs establish high ploidy levels at the onset of sexual development, but the factors that regulate chromosome copy numbers during cell division and growth have been a mystery. We report a novel function of a class of Dicer (Dcl-1)- and RNA-dependent RNA polymerase (RdRP)-dependent endogenous small RNAs in regulating chromosome copy number and gene dosage in O. trifallax Asexually growing populations express an abundant class of 21-nt sRNAs that map to both coding and noncoding regions of most chromosomes. These sRNAs are bound to chromatin and their levels surprisingly do not correlate with mRNA levels. Instead, the levels of these small RNAs correlate with genomic DNA copy number. Reduced sRNA levels in dcl-1 or rdrp mutants lead to concomitant reduction in chromosome copy number. Furthermore, these cells show no signs of transposon activation, but instead display irregular nuclear architecture and signs of replication stress. In conclusion, Oxytricha Dcl-1 and RdRP-dependent small RNAs that derive from the somatic nucleus contribute to the maintenance of gene dosage, possibly via a role in DNA replication, offering a novel role for these small RNAs in eukaryotes. © 2018 Khurana et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  11. Generation and analysis of expressed sequence tags from the ciliate protozoan parasite Ichthyophthirius multifiliis

    Directory of Open Access Journals (Sweden)

    Arias Covadonga

    2007-06-01

    Full Text Available Abstract Background The ciliate protozoan Ichthyophthirius multifiliis (Ich is an important parasite of freshwater fish that causes 'white spot disease' leading to significant losses. A genomic resource for large-scale studies of this parasite has been lacking. To study gene expression involved in Ich pathogenesis and virulence, our goal was to generate expressed sequence tags (ESTs for the development of a powerful microarray platform for the analysis of global gene expression in this species. Here, we initiated a project to sequence and analyze over 10,000 ESTs. Results We sequenced 10,368 EST clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated 9,769 sequences (94.2% success rate. Post-sequencing processing led to 8,432 high quality sequences. Clustering analysis of these ESTs allowed identification of 4,706 unique sequences containing 976 contigs and 3,730 singletons. These unique sequences represent over two million base pairs (~10% of Plasmodium falciparum genome, a phylogenetically related protozoan. BLASTX searches produced 2,518 significant (E-value -5 hits and further Gene Ontology (GO analysis annotated 1,008 of these genes. The ESTs were analyzed comparatively against the genomes of the related protozoa Tetrahymena thermophila and P. falciparum, allowing putative identification of additional genes. All the EST sequences were deposited by dbEST in GenBank (GenBank: EG957858–EG966289. Gene discovery and annotations are presented and discussed. Conclusion This set of ESTs represents a significant proportion of the Ich transcriptome, and provides a material basis for the development of microarrays useful for gene expression studies concerning Ich development, pathogenesis, and virulence.

  12. Doña Clara e don Luis: l’ennesima 'questione amorosa' all’interno della "venta de Palomeque" (Don Quijote, I, 32-47

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    A. Candeloro

    2012-08-01

    Full Text Available ITDoña Clara e don Luis rappresentano l’ultima coppia d’innamorati che raggiunge la locanda di Palomeque, all’interno di quella serie di disavventure e riconoscimenti insperati che danno vita allo “escandaloso happy end” – come lo definirà ironicamente Juan Benet – nel quale si risolve l’azione principale connessa alle numerose trame secondarie  sviluppate proprio nello spazio “scenico” della famosa locanda. Scopo di questo studio è quello di analizzare le caratteristiche e le problematiche legate alla “questione amorosa” di doña Clara e don Luis, il modo in cui nasce e si sviluppa tale intreccio e le tecniche narrative attraverso cui Cervantes ricollega e amalgama questa storia d’amore a quelle delle altre coppie presenti nella conclusione della Primera Parte del Quijote.ENDoña Clara and don Luis are the last couple of lovers arriving to Palomeque's inn. They are the umpteenth romantic couple in a string of episodes based on several recognition scenes between different couples.  That's why they contribute to reach that "scandalous happy end" – as Juan Benet ironically defined it –, through which the main action of the novel's plot comes to a conclusion and allows to finish, at the same time, all the secondary plots at the inn. This report aims to analyze the characteristics and problems related to "the love issue" between Doña Clara and don Luis and, in special, all the particular narrative techniques which Cervantes uses to link this "love story" with other couples in the first part of Don Quijote.Keywords: Cervantes – Don Quijote – narrative techniques – Doña Clara and Don Luis – love question

  13. Cross-study analysis of genomic data defines the ciliate multigenic epiplasmin family: strategies for functional analysis in Paramecium tetraurelia.

    Science.gov (United States)

    Damaj, Raghida; Pomel, Sébastien; Bricheux, Geneviève; Coffe, Gérard; Viguès, Bernard; Ravet, Viviane; Bouchard, Philippe

    2009-06-03

    The sub-membranous skeleton of the ciliate Paramecium, the epiplasm, is composed of hundreds of epiplasmic scales centered on basal bodies, and presents a complex set of proteins, epiplasmins, which belong to a multigenic family. The repeated duplications observed in the P. tetraurelia genome present an interesting model of the organization and evolution of a multigenic family within a single cell. To study this multigenic family, we used phylogenetic, structural, and analytical transcriptional approaches. The phylogenetic method defines 5 groups of epiplasmins in the multigenic family. A refined analysis by Hydrophobic Cluster Analysis (HCA) identifies structural characteristics of 51 epiplasmins, defining five separate groups, and three classes. Depending on the sequential arrangement of their structural domains, the epiplasmins are defined as symmetric, asymmetric or atypical. The EST data aid in this classification, in the identification of putative regulating sequences such as TATA or CAAT boxes. When specific RNAi experiments were conducted using sequences from either symmetric or asymmetric classes, phenotypes were drastic. Local effects show either disrupted or ill-shaped epiplasmic scales. In either case, this results in aborted cell division. Using structural features, we show that 4 epiplasmins are also present in another ciliate, Tetrahymena thermophila. Their affiliation with the distinctive structural groups of Paramecium epiplasmins demonstrates an interspecific multigenic family. The epiplasmin multigenic family illustrates the history of genomic duplication in Paramecium. This study provides a framework which can guide functional analysis of epiplasmins, the major components of the membrane skeleton in ciliates. We show that this set of proteins handles an important developmental information in Paramecium since maintenance of epiplasm organization is crucial for cell morphogenesis.

  14. Cross-study analysis of genomic data defines the ciliate multigenic epiplasmin family: strategies for functional analysis in Paramecium tetraurelia

    Directory of Open Access Journals (Sweden)

    Ravet Viviane

    2009-06-01

    Full Text Available Abstract Background The sub-membranous skeleton of the ciliate Paramecium, the epiplasm, is composed of hundreds of epiplasmic scales centered on basal bodies, and presents a complex set of proteins, epiplasmins, which belong to a multigenic family. The repeated duplications observed in the P. tetraurelia genome present an interesting model of the organization and evolution of a multigenic family within a single cell. Results To study this multigenic family, we used phylogenetic, structural, and analytical transcriptional approaches. The phylogenetic method defines 5 groups of epiplasmins in the multigenic family. A refined analysis by Hydrophobic Cluster Analysis (HCA identifies structural characteristics of 51 epiplasmins, defining five separate groups, and three classes. Depending on the sequential arrangement of their structural domains, the epiplasmins are defined as symmetric, asymmetric or atypical. The EST data aid in this classification, in the identification of putative regulating sequences such as TATA or CAAT boxes. When specific RNAi experiments were conducted using sequences from either symmetric or asymmetric classes, phenotypes were drastic. Local effects show either disrupted or ill-shaped epiplasmic scales. In either case, this results in aborted cell division. Using structural features, we show that 4 epiplasmins are also present in another ciliate, Tetrahymena thermophila. Their affiliation with the distinctive structural groups of Paramecium epiplasmins demonstrates an interspecific multigenic family. Conclusion The epiplasmin multigenic family illustrates the history of genomic duplication in Paramecium. This study provides a framework which can guide functional analysis of epiplasmins, the major components of the membrane skeleton in ciliates. We show that this set of proteins handles an important developmental information in Paramecium since maintenance of epiplasm organization is crucial for cell morphogenesis.

  15. Qualidade de frutos de tomate da cv. Santa Clara, mutante de fruto amarelo e seus híbridos F1 Quality of tomato red fruit, cv. Santa Clara, its yellow mutant and respective F1 hybrid

    Directory of Open Access Journals (Sweden)

    Elizanilda R. do Rêgo

    1999-07-01

    Full Text Available A caracterização de alguns atributos físicos e químicos de frutos de tomate da cv. Santa Clara, um mutante de fruto amarelo, e o híbrido F1 obtido do cruzamento entre eles, foi feita com base nos teores de b-caroteno, licopeno, vitamina C, pH, matéria fresca, matéria seca, diâmetro do fruto, diâmetro do pericarpo, acidez e sólidos solúveis totais dos tecidos loculares e do pericarpo. Os frutos maduros amarelos apresentaram teores reduzidos de b-caroteno, licopeno e vitamina C, enquanto o híbrido apresentou teores semelhantes ao genótipo normal. Tanto o fruto mutante quanto o F1 apresentaram teor de pH menor que o do fruto normal. Não houve diferença significativa quanto a matéria fresca e seca totais, diâmetro do fruto e do pericarpo e acidez. Os teores de sólidos solúveis totais do pericarpo foram inferiores nos frutos do mutante amarelo e F1, em relação ao genótipo normal, enquanto os teores de sólidos solúveis totais da massa locular não diferiram, estatisticamente, entre os genótipos.The determination of some physical and chemical attributes of red fruits from tomato cv. Santa Clara, a yellow mutant and the F1 hybrid, were based on b-carotene, lycopene, vitamin C, pH, fresh and dry weight matter, pericarp and fruit diameter, titrateable acidity and total soluble solids. Ripe fruits of the yellow mutant showed reduced levels of b-carotene, lycopene and vitamin C, while fruits of the F1 hybrid showed levels of carotenoid pigments and vitamin C similar to the red fruit. Both mutant and F1 ripe fruits showed lower pH levels than the red parentals. Total fresh fruit and dry weight matter, pericarp and fruit diameter, and acidity were similar in the three genotypes studied. The total soluble-solids content of the pericarp was lower in the mutant and F1 fruits, whereas their concentration in the locule tissues was similar in all genotypes.

  16. Modelos de perfil de velocidad para evaluación de consistencia del trazado en carreteras de la provincia de Villa Clara, Cuba Speed profile models for evaluation of design consistency in road of the province of Villa Clara, Cuba

    Directory of Open Access Journals (Sweden)

    René A. García Depestre

    2012-08-01

    Full Text Available Entre los aspectos relativos a la carretera que influyen en la accidentalidad, tiene un gran peso el diseño geométrico, internacionalmente el método más empleado para la evaluación del diseño es a partir de la consistencia del trazado con modelos de perfil de velocidades de operación. Cuba no cuenta con modelos propios que consideren las características de las carreteras y los conductores, por lo que es necesario desarrollar modelos de predicción de velocidades para la evaluación de la consistencia del trazado. El desarrollo de modelos de predicción del perfil de velocidades de operación para diferentes condiciones de alineación en carreteras rurales de dos carriles en el contexto de Cuba, se efectúa a partir de características geométricas y velocidades puntuales, con análisis estadístico de las principales variables que relacionan la velocidad con el diseño. Una vez desarrollados los modelos, se aplican a un tramo de carretera declarado como tramo de concentración de accidentes (TCA de la provincia de Villa Clara localizada en la región central de Cuba, los resultados obtenidos confirman la validez de los modelos desarrollados para determinar los perfiles de velocidad de operación y de esta forma, evaluar la consistencia del trazado, con el objetivo de detectar los lugares de mayores dificultades con relación al trazado.Among the aspects that influence road accidents, geometric design is the most relevant. The most used method for evaluating the design all over the world is based on the design consistency with speed profile models of operation. Cuba does not have proprietary models that consider the characteristics of the roads and drivers, so it is necessary to develop predictive models for the evaluation of design consistency. The development of operating speed profile prediction models for different alignment conditions on rural roads with two lanes in the context of Cuba is made from the geometric characteristics and

  17. Potential risk of Mesodinium rubrum bloom in aquaculture area of Dapeng'ao cove, China: diurnal changes in the ciliate community structure in the surface water

    Directory of Open Access Journals (Sweden)

    Huaxue Liu

    2012-02-01

    Full Text Available Diurnal changes in the structure of the ciliate community in surface waters were studied in the aquaculture area of Dapeng'ao cove,China. Two periods of heavy rainfall occurred during the study period, intensifying water column stratification and influencing the water'sproperties. A total of 21 ciliate taxa from 15 genera were identified; the dominant species was Mesodinium rubrum. The maximumabundance of M. rubrum reached 3.92 × 104 indiv. dm-3,contributing 95.1% (mean value to the total ciliate abundance.Diurnal changes in M. rubrum abundance were highly variable, the driving force probably being irradiance and food availability.The results suggest that M. rubrum may form blooms in aquaculture areas when there is a suitable physical regime with enriched nutrients,which is potentially harmful to the fish-farming industry.

  18. Importance and nutritional value of large ciliates for the reproduction of Acartia clausi during the post spring-bloom period in the North Sea

    DEFF Research Database (Denmark)

    Dutz, Jörg; Peters, J.

    2008-01-01

    Shipboard experiments were performed to examine the qualitative importance of large marine microzooplankton for the reproduction of Acartia clausi in the North Sea. Feeding and egg production were compared in 2 treatments in which females were fed natural seston or natural seston selectively...... enriched with large prey (> 20 mu m). The mineral (C, N) and lipid contents of the food suspensions were determined for size-fractionated samples to characterize the nutritional composition of prey. Large oligotrich ciliates and Strobiliidae dominated the seston biomass. Ciliates, particularly...... of the mineral and lipid content confirmed a high seston nutritional quality. The 20 to 48 mu m fraction reflected the composition of female diets and indicated an enrichment of N and polyunsaturated fatty acids (PUFA) in the diet compared to total seston. The fraction > 48 mu m consisted mostly of ciliates...

  19. Use of ciliates (Protozoa: Ciliophora) as bioindicator to assess sediment quality of two constructed mangrove sewage treatment belts in Southern China.

    Science.gov (United States)

    Chen, Qing-Hua; Xu, Run-Lin; Tam, Nora F Y; Cheung, Siu Gin; Shin, Paul K S

    2008-01-01

    To complement physical and chemical data, information of biological communities is important to assess the qualities of mangrove sediments receiving wastewater. Ciliate communities have cosmopolitan distribution, short life cycle and high sensitivity to pollutants, which make them useful as biological indicators of the sediment environment. In most literature, ciliates are widely used as bioindicators for the state of water quality. In this study, the physico-chemical parameters and ciliate community structure of surface sediment collected at different sampling points from two constructed mangrove (Aegiceras and Sonneratia) belts for treatment of municipal sewage in southern China were investigated. Results showed that most (> 80%) of the 216 species ciliates identified at the two constructed mangrove belts were either omnivorous or bacterivorous. Sediment redox potential (Eh) was considered an important factor to govern the distribution of ciliate species within the mangrove sediment. The saprobic system originally derived from freshwater ecosystem was used to evaluate the saprobic degrees of these constructed mangrove belts. Saprobic index (SI) values declined from the sewage inlet to the outlet points of the constructed belts, suggesting better sediment quality at the outlet point caused by treatment processes within the mangrove belt system. Sediment quality of the sewage outlet area of the constructed Aegiceras belt was determined as class II-III (SI = 2.48), while that of the Sonneratia belt was as class III (SI = 2.71) according to the saprobic classification, indicating that a better sewage treatment efficiency was apparent in the Aegiceras than Sonneratia belt. The present data suggested that ciliates could serve as a good bioindicator in assessing organically polluted sediment qualities.

  20. An Integrated Approach to Resolving Structure Associated with the Monte Vista Fault Zone, Southwest Santa Clara Valley, California, Using Geologic, Potential-field and Seismic Data

    Science.gov (United States)

    Langenheim, V. E.; Catchings, R. D.; McLaughlin, R. J.; Jachens, R. C.; Wentworth, C. M.; Stanley, R. G.; Mankinen, E. A.

    2002-12-01

    We integrate geologic and geophysical information to characterize the upper-plate structure of the Monte Vista fault zone (MVFZ). The MVFZ is part of the larger Foothills thrust fault system and extends 25 km from Los Altos southeast to Los Gatos along the front of the range. At the northwestern end of the MVFZ, Franciscan and Miocene sedimentary rocks are thrust over Quaternary deposits. Upper-plate rocks of the Plio-Quaternary Santa Clara Formation are folded, with Miocene rocks exposed in the core of a hanging-wall anticline. The fold axes parallel the southeast strike of the MVFZ for 5-6 km, then diverge southward from the fault zone and disappear near Saratoga, to be replaced by deeply dissected, nearly flat-lying Santa Clara Formation and/or Pleistocene fan deposits. These relations suggest that there is a structural transition within the upper plate of the MVFZ. North of this transition, the Santa Clara Formation may be as thick as 600-700 m, if its surface distribution and bedding attitudes represent uniform thickness across the axis of a prominent synclinal trough. South of the transition, water wells within the undeformed surficial deposits encountered "blue shale" at a depth of about 120 m. If the drillers' "blue shale" is Franciscan basement, this abrupt change in thickness of the young deposits should produce a 10 mGal anomaly that is not seen on a longitudinal gravity profile across the transition. Given this absence, the blue shale is probably part of the Santa Clara Formation, rather than Franciscan basement. North of the transition, the absence of local gravity lows over the thick, downfolded Santa Clara Formation suggests that the Santa Clara is not as thick as projected from the geologic relations or is denser than similar deposits elsewhere in the region. Thickening of the Plio-Quaternary deposits northeastward across the MVFZ is suggested by a magnetic high that extends basinward to the northeast. The gradient of the edge anomaly is only 500

  1. A simple and rapid PCR-based method to isolate complete small macronuclear minichromosomes from hypotrich ciliates: 5S rDNA and S26 ribosomal protein gene of Oxytricha (Sterkiella) nova.

    Science.gov (United States)

    Callejas, Sergio; Gutiérrez, Juan Carlos

    2002-06-01

    Hypotrich ciliates present a macronuclear genome consisting of gene-sized instead of chromosome-sized DNA molecules. Exploiting this unique eukaryotic genome feature, we introduce, for the first time in ciliates, a rapid and easy PCR method using telomeric primers to isolate small complete macronuclear DNA molecules or minichromosomes. Two presumably abundant macronuclear DNA molecules, containing ribosomal genes, were amplified from the Oxytricha (Sterkiella) nova complete genome after using this method, and then were cloned and sequenced. The 5S rDNA sequence of O. (S.) nova is the third one reported among hypotrich ciliates; its primary and secondary structure is compared with other eukaryotic 5S rRNAs. The ribosomal protein S26 gene is the first one reported among ciliates. This "End-End-PCR" method might be useful to obtain similar gene-sized macronuclear molecules from other hypotrich ciliates, and, therefore, to increase our knowledge on ribosomal genes in these eukaryotic microorganisms.

  2. The role of ciliates within the microbial food web in the eutrophicated part of Kaštela Bay (Middle Adriatic Sea

    Directory of Open Access Journals (Sweden)

    Natalia Bojanic

    2006-09-01

    Full Text Available Interactions among phytoplankton, bacterioplankton, heterotrophic nanoflagellates (HNF, ciliated protozoa and copepod nauplii were studied in the eutrophicated part of Kas?tela Bay from May 1998 to November 1999. Special emphasis was placed on relationships between size categories of nonloricate ciliates (NLC and other microbial food web components. Biomasses of phytoplankton and bacteria were primarily influenced by abiotic parameters. Temperature indirectly controlled variation in HNF biomass through the changes in biomass of bacteria and the smaller phytoplankton fraction. Besides HNF, bacterial biomass was affected by the NLC

  3. El sistema lacustre de la Formación Mollar en el depocentro triásico de Santa Clara (provincia de Mendoza, Argentina The lacustrine system of the Mollar Formation in the Triassic Santa Clara Depocenter (Mendoza Province, Argentina

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    Luis A Spalletti

    2009-07-01

    Full Text Available El depocentro triásico de Santa Clara, Cuenca Cuyana, se caracteriza por espesos depósitos generados en ambiente lacustre. Uno de ellos, con un registro de más de 345 m, corresponde a la Formación Mollar. El tramo inferior a medio está caracterizado por una monótona sucesión de lutitas bituminosas acumuladas por decantación suspensiva en el 'off shore' anóxico de un cuerpo lacustre hidrológicamente cerrado y que muestra alto contenido de materia orgánica amorfa de origen algal y de restos de plantas terrestres muy degradadas junto a sulfuros de hierro autígenos. Intercalan areniscas finas debidas a corrientes de turbidez diluidas y carbonatas estromatolíticos. En esta sucesión se definen ciclos granocrecientes de pequeña escala (PACs que se atribuyen a episodios de expansión-retracción lacustre controlados por cambios climáticos. Asociaciones de facies heterolíticas representan los depósitos de la transición entre los ambientes de 'nearshore' y 'offshore' lacustre. La sección superior de la Formación Mollar se compone de sucesiones pelíticas con profusa bioturbación que sugieren mayor oxigenación del sustrato y se asignan a ambiente de costa afuera de un sistema lacustre holomíctico hidrológicamente abierto. Intercalan areniscas producto de flujos hiperpicnales. Depósitos de areniscas con abundantes trazas fósiles y estructuras de olas y flujos unidireccionales, representan a sectores marginales del ambiente lacustre. Además de los ciclos de alta frecuencia, en la Formación Mollar se definen tres secuencias asimétricas de mayor escala atribuidas a episodios mayores de expansión-contracción lacustre debidos a la interacción entre factores climáticos y tectónicos. El diseño de superposición granocreciente de la Formación Mollar y la gradual transición a depósitos fluviales de la sobreyacente Formación Montaña refleja asimismo una constante reducción del espacio de acomodación sedimentaria en el

  4. Proteomic analysis of differentially expressed proteins in the marine fish parasitic ciliate Cryptocaryon irritans.

    Science.gov (United States)

    Mai, Yong-Zhan; Li, Yan-Wei; Li, Rui-Jun; Li, Wei; Huang, Xia-Zi; Mo, Ze-Quan; Li, An-Xing

    2015-06-30

    Cryptocaryoniasis is a severe disease of farmed marine fish caused by the parasitic ciliate Cryptocaryon irritans. This disease can lead to considerable economic loss, but studies on proteins linked to disease development and antigenic proteins for vaccine development have been relatively scarce to date. In this study, 53 protein spots with differential abundance, representing 12 proteins, were identified based on a pair-wise comparison among theronts, trophonts, and tomonts. Meanwhile, 33 protein spots that elicited serological responses in rabbits were identified, representing 9 proteins. In addition, 27 common antigenic protein spots reacted with grouper anti-sera, representing 10 proteins. Most of the identified proteins were involved in cytoskeletal and metabolic pathways. Among these proteins, actin and α-tubulin appeared in all three developmental stages with differences in molecular weights and isoelectric points; 4 proteins (vacuolar ATP synthase catalytic subunit α, mcm2-3-5 family protein, 26S proteasome subunit P45 family protein and dnaK protein) were highly expressed only in theronts; while protein kinase domain containing protein and heat shock protein 70 showed high levels of expression only in trophonts and tomonts, respectively. Moreover, actin was co-detected with 3 rabbit anti-sera while β-tubulin, V-type ATPase α subunit family protein, heat shock protein 70, mitochondrial-type hsp70, and dnaK proteins showed immunoreactivity with corresponding rabbit anti-sera in theronts, trophonts, and tomonts. Furthermore, β-tubulin, the metabolic-related protein enolase, NADH-ubiquinone oxidoreductase 75 kDa subunit, malate dehydrogenase, as well as polypyrimidine tract-binding protein, glutamine synthetase, protein kinase domain containing protein, TNFR/NGFR cysteine-rich region family protein, and vacuolar ATP synthase catalytic subunit α, were commonly detected by grouper anti-sera. Therefore, these findings could contribute to an understanding of

  5. Ultrastructural features of Cryptocaryon irritans, a ciliate parasite of marine fish.

    Science.gov (United States)

    Colorni, A; Diamant, A

    1993-11-12

    The parasitic, reproductive, and free living phases of Cryptocaryon irritans Brown 1951, a ciliate parasite of marine fish, were studied by means of transmission and scanning electron microscopy. The ciliature of this protozoan is arranged in 78-80 monokinetid meridians which run lengthwise converging at the oral cavity and at the posterior pole of the cell. In the trophont, a crown of pointed ciliar triplets fused at the tip delimits a small cytostome whose radially ridged walls lead to a shallow cytopharynx. The trophont feeds on whole host cells and tissue debris. An electron-dense, foam-like, PAS-positive substance fills the pellicular alveoli of the growing trophont. The mechanism of its formation is yet to be determined and several possible functions for it are hypothesized. The macronucleus in the young trophont consists of four linked bead-like segments twisted in a crescent-shaped alignment; up to five micronuclei are adjacently located. At this stage, the macronucleus is homeomeric. Along with trophont growth, the macronucleus increases in volume and its coarse network of chromatin expands. As the trophont leaves the host, development proceeds onto the protomont and tomont stages, during which a substantial reorganization occurs in the cell. The dense chromatin clumps apparently coalesce while the electron-lucent matter expands and the four macronuclear segments fuse into one thick, elongated strand which coils throughout the protoplasm. The micronuclei are no longer detectable in the protomont. The tomont then begins to undergo palintomic division, yielding scores of tomites. In the tomite, the macronuclear chromatin bundles are thin and abundant within the electron-lucent matrix. The micronuclei reappear. Following excystment, the emerging infective theront actively seeks out its host. At this stage its oral apparatus appears as a narrow slit surrounded by cilia shorter than the somatic ones, and is presumably not yet functional. The macronucleus is

  6. Fate of engineered cerium oxide nanoparticles in an aquatic environment and their toxicity toward 14 ciliated protist species.

    Science.gov (United States)

    Zhang, Wei; Pu, Zhichao; Du, Songyan; Chen, Yongsheng; Jiang, Lin

    2016-05-01

    The potential environmental impacts of engineered cerium oxide nanoparticles (CeO2 NPs) on aquatic organisms have remained largely unknown. Therefore, the laboratory study featured herein was performed to determine the fate of CeO2 NPs in an aquatic environment and their toxicity towards 14 different ciliated protist species at a specified population level. An investigation of 48 h aggregation kinetics in the Dryl's solution showed the CeO2 NPs to be relatively stable. The pH values in three test medium were too far away from PZC, which explained the stability of CeO2 NPs. CeO2 NPs generally elicited more toxicity with increasing NP concentration, following certain dose-response relationships. Nano-CeO2 resulted in greater toxicity in a particle state than when added as bulk material. LC50 values showed a negative correlation with the surface-to-volume ratio for these protists, suggesting that surface adsorption of CeO2 NPs might contribute to the observed toxicity. Additionally, acute toxic responses of 14 ciliated protist species to CeO2 NPs were not significantly phylogenetically conserved. The results of these observations provide a better insight into the potential risks of CeO2 NPs in an aquatic environment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. OCT-based quantification of flow velocity, shear force, and power generated by a biological ciliated surface (Conference Presentation)

    Science.gov (United States)

    Huang, Brendan K.; Khokha, Mustafa K.; Loewenberg, Michael; Choma, Michael A.

    2016-03-01

    In cilia-driven fluid flow physiology, quantification of flow velocity, shearing force, and power dissipation is important in defining abnormal ciliary function. The capacity to generate flow can be robustly described in terms of shearing force. Dissipated power can be related to net ATP consumption by ciliary molecular motors. To date, however, only flow velocity can be routinely quantified in a non-invasive, non-contact manner. Additionally, traditional power-based metrics rely on metabolic consumption that reflects energy consumption not just from cilia but also from all active cellular processes. Here, we demonstrate the estimation of all three of these quantities (flow velocity, shear force, and power dissipation) using only optical coherence tomography (OCT). Specifically, we develop a framework that can extract force and power information from vectorial flow velocity fields obtained using OCT-based methods. We do so by (a) estimating the viscous stress tensor from flow velocity fields to estimate shearing force and (b) using the viscous stress tensor to estimate the power dissipation function to infer total mechanical power. These estimates have the advantage of (a) requiring only a single modality, (b) being non-invasive in nature, and (c) being reflective of only the net power work generated by a ciliated surface. We demonstrate our all-optical approach to the estimation of these parameters in the Xenopus animal model system under normal and increased viscous loading. Our preliminary data support the hypothesis that the Xenopus ciliated surface can increase force output under loading conditions.

  8. EVALUATION OF THE RESIZING OF THE WASTE TREATMENT PLANT OF THE TEXTILE COMPANY "DESEMBARCO DEL GRANMA", SANTA CLARA

    Directory of Open Access Journals (Sweden)

    Isabel Cabrera Estrada

    2015-10-01

    Full Text Available The increase of the sewage is a serious global problem. The objective of this work is to make an technical, economic and environmental evaluation of the resizing of the plant for the treatment of waste water from the textile company "Desembarco del Granma", in Santa Clara and to offer a proposal to improve the functioning of the same company, specifically with its process of biological treatment using activated sludge. As a preliminary step, using experimental techniques, the characteristics of the tributary and the effluent from the system were evaluated. COD values higher than 90 mg/L showed adverse implications for the body receiver and an apparent inability of the system to remove organic matter to the levels required. The recalculation of the parameters of design/operation of the plant for the current conditions of the tributary was made. It is obtained that these parameters are not within the recommended values and finally, it was proposed and evaluated the increased to 50 and 75 % of the volume of the aeration tank and the commissioning of two or three of the air vents. With the expansion of the plant's capacity will be achieve a treatment that allows the effluent meet established standards for dumping and therefore avoids damage to the environment and the payment of fines for these concepts. The economic indicators NPV, IRR and PBP calculated, demonstrate the acceptability of the investment required to implement the modifications. All this together proves that the project is deployable.

  9. Radionuclide and heavy metal concentrations in soil, vegetation, and fish collected around and within Tsicoma Lake in Santa Clara Canyon

    Energy Technology Data Exchange (ETDEWEB)

    Fresquez, P.R.; Armstrong, D.R.; Naranjo, L. Jr.

    1996-03-01

    Radionuclide ({sup 3}H, {sup 90}Sr, {sup 137}Cs, {sup 238}Pu, {sup 239}Pu, total U) and heavy metal (Ag, As, Ba, Be, Cd, Cr, Hg, Ni, Pb, Sb, Se, Tl) contents were determined in soil, vegetation (overstory and understory), and fish (rainbow trout) collected around and within Tsicoma Lake in Santa Clara Canyon in 1995. All heavy metal and most radionuclide contents around or within the lake, except for U in soil, vegetation, and fish, were within or just above upper limit background. Detectable levels (where the analytical result was greater than two times counting uncertainty) of U in soils, vegetation, and fish were found in slightly higher concentrations than in background samples. Overall, however, maximum total committed effective dose equivalent (CEDE)(95% confidence level)--based on consumption of 46 lb of fish--from Tsicoma Lake (0.066 mrem/y) was within the maximum total CEDE from the ingestion of fish from the Mescalero National Fish Hatchery (background)(0.113 mrem/y).

  10. Changes in cloud properties over East Asia deduced from the CLARA-A2 satellite data record

    Science.gov (United States)

    Benas, Nikos; Fokke Meirink, Jan; Hollmann, Rainer; Karlsson, Karl-Göran; Stengel, Martin

    2017-04-01

    Studies on cloud properties and processes, and their role in the Earth's changing climate, have advanced during the past decades. A significant part of this advance was enabled by satellite measurements, which offer global and continuous monitoring. Lately, a new satellite-based cloud data record was released: the CM SAF cLoud, Albedo and surface RAdiation dataset from AVHRR data - second edition (CLARA-A2) includes high resolution cloud macro- and micro-physical properties derived from the AVHRR instruments on board NOAA and MetOp polar orbiters. Based on this data record, an analysis of cloud property changes over East Asia during the 12-year period 2004-2015 was performed. Significant changes were found in both optical and geometric cloud properties, including increases in cloud liquid water path and top height. The Cloud Droplet Number Concentration (CDNC) was specifically studied in order to gain further insight into possible connections between aerosol and cloud processes. To this end, aerosol and cloud observations from MODIS, covering the same area and period, were included in the analysis.

  11. PRODUCCIÓN PRIMARIA EN UN LAGO DE AGUAS CLARAS DE LA AMAZONÍA COLOMBIANA (LAGO BOA

    Directory of Open Access Journals (Sweden)

    GABRIEL ANTONIO PINILLA AGUDELO

    2009-01-01

    Full Text Available Dentro de los aspectos funcionales de un sistema acuático uno de los más determinantes es la producción fitoplanctónica. En este trabajo se midió la producción primaria del fitoplancton (método del 14C en un lago de aguas claras de la Amazonía colombiana, en diferentes momentos del ciclo hidrológico. La producción primaria osciló entre 0,6 y 2,3 g C m-2 d-1, valor este último medido durante la fase de aguas bajas. Estos resultados indican que la comunidad fitoplanctónica del lago Boa fluctúa desde condiciones de muy baja capacidad de asimilación de carbono inorgánico (aguas altas, hasta momentos en que este potencial se incrementa hasta cuatro veces (aguas bajas. Tales cambios drásticos obedecen a las fluctuaciones en la abundancia de microalgas, las que a su vez se presentan como respuesta a la variación trófica que tiene el lago durante el ciclo hidrológico. De esta manera, se pasa de condiciones oligotróficas en la época de inundación a un estado eutrófico cuando el sistema se desconecta del río en aguas bajas.

  12. Caracterización de las farmacias comunitarias de Santa Clara para la implementación de la Dispensación de Medicamentos

    Directory of Open Access Journals (Sweden)

    Liset Jaramillo Hernández

    2012-03-01

    Full Text Available Introducción: la inclusión del Servicio de Dispensación de Medicamentos en la Atención Farmacéutica, busca evidenciar la participación del farmacéutico en el logro de resultados positivos en los tratamientos, basado en los conocimientos farmacoterapéuticos y principios éticos que identifican esta profesión. La caracterización de las farmacias comunitarias resulta elemental para trazar acciones que permitan implementar dicho servicio. Objetivo: caracterizar las farmacias comunitarias de Santa Clara para la implementación del Servicio de Dispensación de Medicamentos. Métodos: se realizó una investigación en sistemas y servicios de salud con un enfoque sistémico (evaluación de la estructura, el proceso y los resultados. El estudio fue observacional, descriptivo y transversal. El contexto espacial lo constituyeron las farmacias comunitarias del municipio de Santa Clara, en el período comprendido entre enero y mayo de 2010. Se seleccionó el 30 % del total de farmacias comunitarias del municipio de Santa Clara. Resultados: la disponibilidad de los recursos humanos fue limitada y no existieron suficientes recursos materiales para sustentar el servicio. El 95,2 % de los farmacéuticos comunitarios presentaron altas necesidades de aprendizaje sobre Dispensación y no existieron Procedimientos Normalizados de Trabajo. A través de la observación directa se determinó que el servicio no se ejecutó, lo cual se corroboró con la encuesta realizada a los usuarios. Conclusiones: las farmacias comunitarias del municipio de Santa Clara se caracterizan por tener una estructura parcialmente adecuada y por ser el proceso y los resultados no adecuados para la ejecución del Servicio de Dispensación de Medicamentos.

  13. EVIDENCIA DE ESCORIAS DE COBRE PREHISPÁNICAS EN EL ÁREA DE SANTA CLARA DEL COBRE, MICHOACÁN, OCCIDENTE DE MÉXICO (Evidence of Prehispanic Copper Slags from the Santa Clara del Cobre Area, Michoacan, Western Mexico)

    National Research Council Canada - National Science Library

    José Luis Punzo Díaz; Juan Morales; Avto Goguitchaichvili

    2015-01-01

    ... arqueomagnético de las escorias, producto de la fundición del cobre en siete sitios arqueológicos de los alrededores de Santa Clara del Cobre, Michoacán, se logró ubicarlas cronológicamente...

  14. Application of seismic refraction tomography for tunnel design in Santa Clara Mountain, San Juan, Argentina Application of seismic refraction tomography for tunnel design in Santa Clara Mountain, San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Imhof Armando Luis

    2011-12-01

    Full Text Available

    A geophysical survey involving seismic refraction tomography (SRT for mapping 'P' waves was carried out in Sierra Santa Clara, San Juan Province, Argentina in July 2009. The purpose of the geophysical survey was to determine the degree of fracturing and the rigidity of the rock mass through which it is planned to build a 290 m long road tunnel traversing the mountain almost perpendicular to the axis thereof, at around 100 m depth from the summit.

    Several difficulties arose from the operational point of view which made it almost impossible to conduct fieldwork in normal circumstances. Firstly, the topography had almost 45° slopes and 100 m research depths which would have involved having had to use explosives to generate seismic waves reaching sensors which had sufficient signal-to-noise ratio for distinguishing them. Legal restrictions regarding the use of explosives on the one hand and insufficient power when using hammer blows on the other made it necessary to design and build a gas-powered gun to achieve the minimum energy (2 kJ required for detecting seismic signals.

    Secondly, using conventional interpretation methods involving layered models was inoperable in such geological structures; seismic tomography methods were thus used which make use of the velocity gradient concept (both lateral and in-depth. This allowed mapping subsurface velocity variations in the form of velocity contour lines.

    The methodology used with the new seismic waves' source generator, as well as SRT application in this type of geological structure, demonstrated that satisfactory results could be obtained for this kind of geophysical study for geotechnical purposes.

    A geophysical survey involving seismic refraction tomography (SRT for mapping 'P' waves was

  15. Draft genome sequences of three Holospora species (Holospora obtusa, Holospora undulata, and Holospora elegans), endonuclear symbiotic bacteria of the ciliate Paramecium caudatum.

    Science.gov (United States)

    Dohra, Hideo; Tanaka, Kenya; Suzuki, Tomohiro; Fujishima, Masahiro; Suzuki, Haruo

    2014-10-01

    We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be essential for the infection and survival in the host nucleus.

  16. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2009

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2009 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  17. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2007

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2007 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  18. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2005 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  19. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2008

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2008 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  20. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2004

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2004 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  1. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2010

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2010 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  2. Don Edwards San Francisco Bay National Wildlife Refuge : Self Monitoring Program for Alviso Ponds Within South San Francisco Bay Low Salinity Salt Ponds Alameda, Santa Clara, and San Mateo Counties, California : 2006

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual report summarizes the results of the 2006 water quality sampling conducted at the Alviso Ponds in Santa Clara County, California, which are part of the...

  3. Un peu de désordre n'est pas du tout désagréable Clara Gaspar se passionne pour son métier au CERN, sans jamais oublier son Portugal

    CERN Multimedia

    Imhof, Michel

    2002-01-01

    Clara Gaspar is passionately fond of her job at CERN and enjoys the enrichment of life being in an international community. She misses some things and will never forget Portugal but counts Geneva as her home (2 pagees)

  4. Quantification and visualization of injury and regeneration in the developing ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Gamm, Ute A.; Huang, Brendan K.; Mis, Emily K.; Khokha, Mustafa K.; Choma, Michael A.

    2017-02-01

    Premature infants are at a high risk for respiratory diseases owing to an underdeveloped respiratory system that is very susceptible to infection and inflammation. One aspect of respiratory health is the state of the ciliated respiratory epithelium which lines the trachea and bronchi. The ciliated epithelium is responsible for trapping and removing pathogens and pollutants from the lungs and an impairment of ciliary functionality can lead to recurring respiratory infections and subsequent lung damage. Mechanisms of cilia-driven fluid flow itself but also factors influenced by development like ciliary density and flow generation are incompletely understood. Furthermore, medical interventions like intubation and accidental aspiration can lead to focal or diffuse loss of cilia and disruption of flow. In this study we use two animal models, Xenopus embryo and ex vivo mouse trachea, to analyze flow defects in the injured ciliated epithelium. Injury is generated either mechanically with a scalpel or chemically by calcium chloride (CaCl2) shock, which efficiently but reversibly deciliates the embryo skin. In this study we used optical coherence tomography (OCT) and particle tracking velocimetry (PTV) to quantify cilia driven fluid flow over the surface of the Xenopus embryo. We additionally visualized damage to the ciliated epithelium by capturing 3D speckle variance images that highlight beating cilia. Mechanical injury disrupted cilia-driven fluid flow over the injured site, which led to a reduction in cilia-driven fluid flow over the whole surface of the embryo (n=7). The calcium chloride shock protocol proved to be highly effective in deciliating embryos (n=6). 3D speckle variance images visualized a loss of cilia and cilia-driven flow was halted immediately after application. We also applied CaCl2-shock to cultured ex vivo mouse trachea (n=8) and found, similarly to effects in Xenopus embryo, an extensive loss of cilia with resulting cessation of flow. We

  5. Ex vivo visualization of human ciliated epithelium and quantitative analysis of induced flow dynamics by using optical coherence tomography.

    Science.gov (United States)

    Ling, Yuye; Yao, Xinwen; Gamm, Ute A; Arteaga-Solis, Emilio; Emala, Charles W; Choma, Michael A; Hendon, Christine P

    2017-03-01

    Cilia-driven mucociliary clearance is an important self-defense mechanism of great clinical importance in pulmonary research. Conventional light microscopy possesses the capability to visualize individual cilia and its beating pattern but lacks the throughput to assess the global ciliary activities and flow dynamics. Optical coherence tomography (OCT), which provides depth-resolved cross-sectional images, was recently introduced to this area. Fourteen de-identified human tracheobronchial tissues are directly imaged by two OCT systems: one system centered at 1,300 nm with 6.5 μm axial resolution and 15 μm lateral resolution, and the other centered at 800 nm with 2.72 μm axial resolution and 5.52 μm lateral resolution. Speckle variance images are obtained in both cross-sectional and volumetric modes. After imaging, sample blocks are sliced along the registered OCT imaging plane and processed with hematoxylin and eosin (H&E) stain for comparison. Quantitative flow analysis is performed by tracking the path-lines of microspheres in a fixed cross-section. Both the flow rate and flow direction are characterized. The speckle variance images successfully segment the ciliated epithelial tissue from its cilia-denuded counterpart, and the results are validated by corresponding H&E stained sections. A further temporal frequency analysis is performed to extract the ciliary beat frequency (CBF) at cilia cites. By adding polyester microspheres as contrast agents, we demonstrate ex vivo imaging of the flow induced by cilia activities of human tracheobronchial samples. This manuscript presents an ex vivo study on human tracheobronchial ciliated epithelium and its induced mucous flow by using OCT. Within OCT images, intact ciliated epithelium is effectively distinguished from cilia-denuded counterpart, which serves as a negative control, by examining the speckle variance images. The cilia beat frequency is extracted by temporal frequency analysis. The flow rate, flow

  6. Simulated ground motion in Santa Clara Valley, California, and vicinity from M≥6.7 scenario earthquakes

    Science.gov (United States)

    Harmsen, Stephen C.; Hartzell, Stephen

    2008-01-01

    Models of the Santa Clara Valley (SCV) 3D velocity structure and 3D finite-difference software are used to predict ground motions from scenario earthquakes on the San Andreas (SAF), Monte Vista/Shannon, South Hayward, and Calaveras faults. Twenty different scenario ruptures are considered that explore different source models with alternative hypocenters, fault dimensions, and rupture velocities and three different velocity models. Ground motion from the full wave field up to 1 Hz is exhibited as maps of peak horizontal velocity and pseudospectral acceleration at periods of 1, 3, and 5 sec. Basin edge effects and amplification in sedimentary basins of the SCV are observed that exhibit effects from shallow sediments with relatively low shear-wave velocity (330 m/sec). Scenario earthquakes have been simulated for events with the following magnitudes: (1) M 6.8–7.4 Calaveras sources, (2) M 6.7–6.9 South Hayward sources, (3) M 6.7 Monte Vista/Shannon sources, and (4) M 7.1–7.2 Peninsula segment of the SAF sources. Ground motions are strongly influenced by source parameters such as rupture velocity, rise time, maximum depth of rupture, hypocenter, and source directivity. Cenozoic basins also exert a strong influence on ground motion. For example, the Evergreen Basin on the northeastern side of the SCV is especially responsive to 3–5-sec energy from most scenario earthquakes. The Cupertino Basin on the southwestern edge of the SCV tends to be highly excited by many Peninsula and Monte Vista fault scenarios. Sites over the interior of the Evergreen Basin can have long-duration coda that reflect the trapping of seismic energy within this basin. Plausible scenarios produce predominantly 5-sec wave trains with greater than 30 cm/sec sustained ground-motion amplitude with greater than 30 sec duration within the Evergreen Basin.

  7. The giant ciliate Zoothamnium niveum and its thiotrophic epibiont Candidatus Thiobios zoothamnicoli: a model system to study interspecies cooperation

    Directory of Open Access Journals (Sweden)

    Monika eBright

    2014-04-01

    Full Text Available Symbioses between chemoautotrophic sulfur-oxidizing (thiotrophic bacteria and protists or animals are among the most diverse and prevalent in the ocean. They are extremely difficult to maintain in aquaria and no thiotrophic symbiosis involving an animal host has ever been successfully cultivated. In contrast, we have cultivated the giant ciliate Zoothamnium niveum and its obligate ectosymbiont Cand. Thiobios zoothamnicoli in small flow-through aquaria. This review provides an overview of the host and the symbiont and their phylogenetic relationships. We summarize our knowledge on the ecology, geographic distribution and life cycle of the host, on the vertical transmission of the symbiont, and on the cultivation of this symbiosis. We then discuss the benefits and costs involved in this cooperation compared with other thiotrophic symbioses and outline our view on the evolution and persistence of this byproduct mutualism.

  8. AON-mediated Exon Skipping Restores Ciliation in Fibroblasts Harboring the Common Leber Congenital Amaurosis CEP290 Mutation

    Directory of Open Access Journals (Sweden)

    Xavier Gerard

    2012-01-01

    Full Text Available Leber congenital amaurosis (LCA is a severe hereditary retinal dystrophy responsible for congenital or early-onset blindness. The most common disease-causing mutation (>10% is located deep in intron 26 of the CEP290 gene (c.2991+1655A>G. It creates a strong splice donor site that leads to insertion of a cryptic exon encoding a premature stop codon. In the present study, we show that the use of antisense oligonucleotides (AONs allow an efficient skipping of the mutant cryptic exon and the restoration of ciliation in fibroblasts of affected patients. These data support the feasibility of an AON-mediated exon skipping strategy to correct the aberrant splicing.

  9. Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China

    Science.gov (United States)

    Chen, Xiangrui; Warren, Alan; Song, Weibo

    2009-12-01

    The morphology and infraciliature of a new marine colepid ciliate, Apocoleps magnus gen. nov., spec. nov., are described based on living observations and silver impregnations. The new genus Apocoleps is characterized by having 8 (vs. 6 in most other related genera) armour tiers, spines at both ends of the cell, 3 adoral organelles and plates with 4 reniform uni-windows. Apocoleps magnus spec. nov. is defined by the following features: body elongated and slightly curved, about 100-120µm× 35-45 µm in vivo; anterior tertiary tier plate with four uni-windows, most secondary and main tier plates with four uni-windows, posterior tertiary tier plate with two uni-windows; left plate margin slightly serrated; on average 23 transverse and 22 longitudinal ciliary rows; one terminal contractile vacuole; marine habitat.

  10. The behaviour of both Listeria monocytogenes and rat ciliated ependymal cells is altered during their co-culture.

    Directory of Open Access Journals (Sweden)

    Mina J Fadaee-Shohada

    Full Text Available BACKGROUND: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis. PRINCIPAL FINDINGS: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle. CONCLUSION: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA.

  11. The symbiotic life of Symbiodinium in the open ocean within a new species of calcifying ciliate (Tiarina sp.).

    Science.gov (United States)

    Mordret, Solenn; Romac, Sarah; Henry, Nicolas; Colin, Sébastien; Carmichael, Margaux; Berney, Cédric; Audic, Stéphane; Richter, Daniel J; Pochon, Xavier; de Vargas, Colomban; Decelle, Johan

    2016-06-01

    Symbiotic partnerships between heterotrophic hosts and intracellular microalgae are common in tropical and subtropical oligotrophic waters of benthic and pelagic marine habitats. The iconic example is the photosynthetic dinoflagellate genus Symbiodinium that establishes mutualistic symbioses with a wide diversity of benthic hosts, sustaining highly biodiverse reef ecosystems worldwide. Paradoxically, although various species of photosynthetic dinoflagellates are prevalent eukaryotic symbionts in pelagic waters, Symbiodinium has not yet been reported in symbiosis within oceanic plankton, despite its high propensity for the symbiotic lifestyle. Here we report a new pelagic photosymbiosis between a calcifying ciliate host and the microalga Symbiodinium in surface ocean waters. Confocal and scanning electron microscopy, together with an 18S rDNA-based phylogeny, showed that the host is a new ciliate species closely related to Tiarina fusus (Colepidae). Phylogenetic analyses of the endosymbionts based on the 28S rDNA gene revealed multiple novel closely related Symbiodinium clade A genotypes. A haplotype network using the high-resolution internal transcribed spacer-2 marker showed that these genotypes form eight divergent, biogeographically structured, subclade types that do not seem to associate with any benthic hosts. Ecological analyses using the Tara Oceans metabarcoding data set (V9 region of the 18S rDNA) and contextual oceanographic parameters showed a global distribution of the symbiotic partnership in nutrient-poor surface waters. The discovery of the symbiotic life of Symbiodinium in the open ocean provides new insights into the ecology and evolution of this pivotal microalga and raises new hypotheses about coastal pelagic connectivity.

  12. Natural products from aquatic eukaryotic microorganisms for cancer therapy: Perspectives on anti-tumour properties of ciliate bioactive molecules.

    Science.gov (United States)

    Catalani, Elisabetta; Proietti Serafini, Francesca; Zecchini, Silvia; Picchietti, Simona; Fausto, Anna Maria; Marcantoni, Enrico; Buonanno, Federico; Ortenzi, Claudio; Perrotta, Cristiana; Cervia, Davide

    2016-11-01

    Several modern drugs, including those for cancer therapy, have been isolated from natural sources, are based on natural products and its derivatives, or mime natural products. Some of them are in clinical use, others in clinical trials. The success of natural products in drug discovery is related to their biochemical characteristics and to the technologic methods used to study their feature. Natural compounds may acts as chemo-preventive agents and as factors that increase therapeutic efficacy of existing drugs, thus overcoming cancer cell drug resistance that is the main factor determining the failure in conventional chemotherapy. Water environment, because of its physical and chemical conditions, shows an extraordinary collection of natural biological substances with an extensive structural and functional diversity. The isolation of bioactive molecules has been reported from a great variety of aquatic organisms; however, the therapeutic application of molecules from eukaryotic microorganisms remains inadequately investigated and underexploited on a systematic basis. Herein we describe the biological activities in mammalian cells of selected substances isolated from ciliates, free-living protozoa common almost everywhere there is water, focusing on their anti-tumour actions and their possible therapeutic activity. In particular, we unveil the cellular and molecular machine mediating the effects of cell type-specific signalling protein pheromone Er-1 and secondary metabolites, i.e. euplotin C and climacostol, in cancer cells. To support the feasibility of climacostol-based approaches, we also present novel findings and report additional mechanisms of action using both in vitro and in vivo models of mouse melanomas, with the scope of highlighting new frontiers that can be explored also in a therapeutic perspective. The high skeletal chemical difference of ciliate compounds, their sustainability and availability, also through the use of new organic synthesis

  13. Anti-parasitic effects of Leptomycin B isolated from Streptomyces sp. CJK17 on marine fish ciliate Cryptocaryon irritans.

    Science.gov (United States)

    Yin, Fei; Sun, Peng; Tang, Baojun; Gong, Hui; Ke, Qiaozhen; Li, Anxing

    2016-02-15

    The present study was conducted aiming to evaluate the in vitro and in vivo anti-parasitic efficacy of an isolated compound against the ciliate Cryptocaryon irritans. The compound was previously isolated from fermentation products of Streptomyces sp. CJK17 and designated as SFrD. Toxicity of the compound SFrD against the fish hosts (Larimichthys crocea) was also tested and its chemical structure was elucidated. The obtained results showed that the compound has potent anti-parasitic efficacy with the 10 min-, 1 h-, 2 h-, 3 h- and 4 h-LC50 (95% Confidence Intervals) of 6.8 (6.5-7.1), 3.9 (2.8-5.0), 3.3 (2.6-4.0), 2.7 (2.3-3.1) and 2.5 (2.2-2.8) mg L(-1) against theronts of C. irritans and the 6h-LC50 (95% CI) of 3.0 (2.8-3.2) mg L(-1) against the tomonts, respectively. Exposure of the compound SFrD remarkably reduced the mortality of fish infected with C. Irritans, from 100% in the control group to 61.7% and 38.3% in groups of 3.1 mg L(-1) and 6.3 mg L(-1), respectively. In the test of exposing fish to 40 mg L(-1) compound SFrD for 24h, no visible effects were observed affecting the normal behavior or any macroscopic changes. By spectrum analysis (EI-MS, (1)H NMR and (13)C NMR), the compound SFrD was identified as Leptomycin B. This study firstly demonstrated that Leptomycin B has potent anti-parasitic efficacy against ciliates in cultured marine fish. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. ORTHOIMAGERY, Santa Clara, CA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Digital orthographic imagery datasets contain georeferenced images of the Earth's surface, collected by a sensor in which object displacement has been removed for...

  15. Santa Clara County, California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LAS format files, raw LiDAR data in its native format, classified bare-earth LiDAR DEM and photogrammetrically derived breaklines generated from LiDAR Intensity...

  16. Quantification and visualization of injury and regeneration to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography (Conference Presentation)

    Science.gov (United States)

    Gamm, Ute A.; Huang, Brendan K.; Mis, Emily K.; Khokha, Mustafa K.; Choma, Michael A.

    2017-04-01

    Mucociliary flow is an important defense mechanism in the lung to remove inhaled pathogens and pollutants. A disruption of ciliary flow can lead to respiratory infections. Even though patients in the intensive care unit (ICU) either have or are very susceptible to respiratory infections, mucociliary flow is not well understood in the ICU setting. We recently demonstrated that hyperoxia, a consequence of administering supplemental oxygen to a patient in respiratory failure, can lead to a significant reduction of cilia-driven fluid flow in mouse trachea. There are other factors that are relevant to ICU medicine that can damage the ciliated tracheal epithelium, including inhalation injury and endotracheal tube placement. In this study we use two animal models, Xenopus embryo and ex vivo mouse trachea, to analyze flow defects in the injured ciliated epithelium. Injury is generated either mechanically with a scalpel or chemically by calcium chloride (CaCl2) shock, which efficiently but reversibly deciliates the embryo skin. In this study we used optical coherence tomography (OCT) and particle tracking velocimetry (PTV) to quantify cilia driven fluid flow over the surface of the Xenopus embryo. We additionally visualized damage to the ciliated epithelium by capturing 3D speckle variance images that highlight beating cilia. Mechanical injury disrupted cilia-driven fluid flow over the injured site, which led to a reduction in cilia-driven fluid flow over the whole surface of the embryo (n=7). The calcium chloride shock protocol proved to be highly effective in deciliating embryos (n=6). 3D speckle variance images visualized a loss of cilia and cilia-driven flow was halted immediately after application. We also applied CaCl2-shock to cultured ex vivo mouse trachea (n=8) and found, similarly to effects in Xenopus embryo, an extensive loss of cilia with resulting cessation of flow. We investigated the regeneration of the ciliated epithelium after an 8 day incubation period

  17. Association of serum Clara cell protein CC16 with respiratory infections and immune response to respiratory pathogens in elite athletes

    Science.gov (United States)

    2014-01-01

    Background Respiratory epithelium integrity impairment caused by intensive exercise may lead to exercise-induced bronchoconstriction. Clara cell protein (CC16) has anti-inflammatory properties and its serum level reflects changes in epithelium integrity and airway inflammation. This study aimed to investigate serum CC16 in elite athletes and to seek associations of CC16 with asthma or allergy, respiratory tract infections (RTIs) and immune response to respiratory pathogens. Methods The study was performed in 203 Olympic athletes. Control groups comprised 53 healthy subjects and 49 mild allergic asthmatics. Serum levels of CC16 and IgG against respiratory viruses and Mycoplasma pneumoniae were assessed. Allergy questionnaire for athletes was used to determine symptoms and exercise pattern. Current versions of ARIA and GINA guidelines were used when diagnosing allergic rhinitis and asthma, respectively. Results Asthma was diagnosed in 13.3% athletes, of whom 55.6% had concomitant allergic rhinitis. Allergic rhinitis without asthma was diagnosed in 14.8% of athletes. Mean CC16 concentration was significantly lower in athletes versus healthy controls and mild asthmatics. Athletes reporting frequent RTIs had significantly lower serum CC16 and the risk of frequent RTIs was more than 2-fold higher in athletes with low serum CC16 (defined as equal to or less than 4.99 ng/ml). Athletes had significantly higher anti-adenovirus IgG than healthy controls while only non-atopic athletes had anti-parainfluenza virus IgG significantly lower than controls. In all athletes weak correlation of serum CC16 and anti-parainfluenza virus IgG was present (R = 0.20, p athletes a weak positive correlations of CC16 with IgG specific for respiratory syncytial virus (R = 0.29, p = 0.009), parainfluenza virus (R = 0.31, p = 0.01) and adenovirus (R = 0.27, p = 0.02) were seen as well. Conclusions Regular high-load exercise is associated with decrease in serum CC16

  18. California GAMA Program: Sources and transport of nitrate in shallow groundwater in the Llagas Basin of Santa Clara County, California

    Energy Technology Data Exchange (ETDEWEB)

    Moran, J E; McNab, W; Esser, B; Hudson, G; Carle, S; Beller, H; Kane, S; Tompson, A B; Letain, T; Moore, K; Eaton, G; Leif, R; Moody-Bartel, C; Singleton, M

    2005-06-29

    A critical component of the State Water Resource Control Board's Groundwater Ambient Monitoring and Assessment (GAMA) Program is to assess the major threats to groundwater resources that supply drinking water to Californians (Belitz et al., 2004). Nitrate is the most pervasive and intractable contaminant in California groundwater and is the focus of special studies under the GAMA program. This report presents results of a study of nitrate contamination in the aquifer beneath the cities of Morgan Hill and Gilroy, CA, in the Llagas Subbasin of Santa Clara County, where high nitrate levels affect several hundred private domestic wells. The main objectives of the study are: (1) to identify the main source(s) of nitrate that issue a flux to the shallow regional aquifer (2) to determine whether denitrification plays a role in the fate of nitrate in the subbasin and (3) to assess the impact that a nitrate management plan implemented by the local water agency has had on the flux of nitrate to the regional aquifer. Analyses of 56 well water samples for major anions and cations, nitrogen and oxygen isotopes of nitrate, dissolved excess nitrogen, tritium and groundwater age, and trace organic compounds, show that synthetic fertilizer is the most likely source of nitrate in highly contaminated wells, and that denitrification is not a significant process in the fate of nitrate in the subbasin except in the area of recycled water application. In addition to identifying contaminant sources, these methods offer a deeper understanding of how the severity and extent of contamination are affected by hydrogeology and groundwater management practices. In the Llagas subbasin, the nitrate problem is amplified in the shallow aquifer because it is highly vulnerable with high vertical recharge rates and rapid lateral transport, but the deeper aquifers are relatively more protected by laterally extensive aquitards. Artificial recharge delivers low-nitrate water and provides a means of

  19. Psychometric properties of an index of three patient reported outcome (PRO) measures, termed the CLinical ARthritis Activity (PRO-CLARA) in patients with rheumatoid arthritis. The NEW INDICES study.

    Science.gov (United States)

    Salaffi, F; Migliore, A; Scarpellini, M; Corsaro, S M; Laganà, B; Mozzani, F; Varcasia, G; Pusceddu, M; Pomponio, G; Romeo, N; Maier, A; Foti, Rosario; Scarpa, Raffaele; Gasparini, Stefania; Bombardieri, Stefano

    2010-01-01

    To evaluate the psychometric properties of an index based on 3 patient reported outcomes measures, termed PRO-CLinical ARthritis Activity (PRO-CLARA), in order to facilitate rapid and easy rheumatoid arthritis (RA) activity assessment in daily routine. 196 patients partially or not responding to disease modifying anti-rheumatic drugs (DMARDs), consented to participate in a multicentre cross-sectional study. For the evaluation of the psychometric properties of the PRO-CLARA, this population has been compared to another cohort of 247 outpatients with RA who were participating in a long-term observational study and who satisfying minimal disease activity and remission definitions. All patients completed the PRO-CLARA, combining patient's physical function, self-administered tender joint count and perception of global health status into a single measure of disease activity. Additional comparator composite indices were analysed. Internal consistency was assessed with Cronbach's alpha coefficient. A confirmatory factor analysis was carried out to test factor structure. Concurrent validity was analyzed using Spearman's correlations and cross-tabulations. Discriminant validity to distinguish patients with active and non-active disease was assessed with receiver operating characteristic (ROC) curve analysis. For agreement analysis, kappa statistics were calculated. In testing for internal consistency, we found that Cronbach's alpha for the PRO-CLARA was 0.893, indicating high reliability. PRO-CLARA proved to be significantly correlated to established RA activity assessment tools. The area under ROC curve of the PRO-CLARA gives identical results to those provided by other comparator indices. The study showed satisfactory psychometric properties of the PRO-CLARA.

  20. Leonor de Sicilia y Santa Clara de Teruel: la fundación reginal de un convento de clarisas y su primer desarrollo

    Directory of Open Access Journals (Sweden)

    Roebert, Sebastian

    2014-06-01

    Full Text Available The history of the monastery of the Poor Clares of Teruel, also known as Santa Catalina or Santa Clara de Teruel, is virtually unknown. One reason for the lack of studies dedicated to this convent is the complete loss of its archive during the Spanish Civil War. The present study intends to reconstruct the foundation and initial development of Santa Clara de Teruel from its beginning in 1367 until the fi rst quarter of the 15th century, using mostly hitherto unknown sources from other archives. Special attention will be paid to the founder of the monastery, Leonor of Sicily (1349-1375, queen of the Crown of Aragon.La historia del monasterio de las clarisas de Teruel, conocido tanto por el nombre de Santa Catalina como por el de Santa Clara de Teruel, nos resulta hasta el día de hoy prácticamente desconocida. Seguramente, este hecho se debe en buena medida a la destrucción completa del archivo conventual durante la Guerra Civil. El presente estudio pretende reconstruir el proceso fundacional y analizar el primer desarrollo del monasterio, desde su creación en el año 1367 hasta el primer cuarto del siglo XV, a través de la documentación que se conserva en otros archivos y que permanece, en su mayor parte, inédita y sin estudiar. Además, se investigará el papel preponderante que desempeñó la fundadora y principal promotora del convento, Leonor de Sicilia (1349-1375, reina de la Corona de Aragón.

  1. Near-surface Faults and Structure of the Western Santa Clara Valley, California as Seen From High-Resolution Seismic Reflection and Refraction Images

    Science.gov (United States)

    Catchings, R. D.; Goldman, M. R.; Gandhok, G.; Langenheim, V. E.

    2002-12-01

    The U.S.G.S acquired an ~10-km-long, high-resolution seismic reflection/refraction profile across the western Santa Clara Valley in the fall of 2000. The seismic profile, which originated within Franciscian rocks of the Santa Cruz Mountains and extended across the Cupertino basin to downtown San Jose, was designed to image shallow (upper 500 m) subsurface structure and stratigraphy along its length. The seismic profile crossed the Monte Vista fault zone and other buried faults inferred to exist beneath the western Santa Clara Valley. High resolution images were provided by seismic sources and geophones that were spaced at 5-m increments along the entire profile, resulting in CDP intervals of 2.5 m. The data were recorded on a 240-channel moving array, which yielded maximum folds over 200 along many segments of the profile. Both velocity and reflection images were developed from the data. Near-surface velocities range from about 500 m/s in the shallow subsurface of the Cupertino basin to more than 3000 m/s in surficial Franciscian rocks of the Santa Cruz Mountains. Reflectivity along the profile varies with rock types, with more reflective strata associated with sediments of the Cupertino basin than Franciscian rocks rocks of the Santa Cruz Mountains. Small offset faults and folds are apparent at several locations along the profile. Larger-offset, south-dipping faults are apparent within the Monte Vista fault zone and at least one other fault zone near the central Santa Clara Valley. The high-resolution images show that the faults extend to the near-surface, suggesting that recency of faulting may be accessed with paleseismological methods. If active, these faults may pose significant hazards to the Santa Clara Valley because they are located in highly populated areas. Furthermore, thick, low-velocity sediments observed in high-population areas along the parts of the profile may amplify seismic waves generated by movement on either local or regional faults, posing

  2. ESTUDIO EMPÍRICO SOBRE LAS ESTRATEGIAS DE LOGÍSTICA INVERSA EN EL SECTOR INDUSTRIAL DE LA PROVINCIA DE VILLA CLARA

    OpenAIRE

    Roberto Cespón Castro; Michael Feitó Cespón

    2009-01-01

    El presente artículo contiene los principales resultados obtenidos de un estudio empírico sobre logística inversa en el sector industrial de la provincia de Villa Clara. En el mismo se parte de la hipótesis de que es posible identificar una taxonomía de estrategias de logística inversa que se ...

  3. Desfronde y tasa de Descomposición foliar en rebollar-pinar de repoblación con distinto grado de clara

    OpenAIRE

    Bravo-Oviedo, Andrés; del Río Gaztelurrutia, Miren; Montero González, Gregorio; Onrubia Fernández, Raquel; Rubio Cuadrado, Álvaro; Ruiz-Peinado, Ricardo

    2013-01-01

    El desfronde representa un factor clave en el funcionamiento de los ecosistemas forestales. Por un lado representa el mayor aporte de nutrientes en sistemas naturales y, por otro, su acumulación en el suelo constituye un banco de elementos esenciales que, por descomposición y mineralización, se integran en el suelo y en los ciclos biogeoquímicos. Este trabajo analiza el efecto de la clara en la producción de desfronde, la eficiencia en el uso del nitrógeno y en la tasa de descomposición de la...

  4. Radionuclides and heavy metals in rainbow trout from Tsichomo, Nana Ka, Wen Povi, and Pin De Lakes in Santa Clara Canyon

    Energy Technology Data Exchange (ETDEWEB)

    Fresquez, P.R.; Armstrong, D.R.; Naranjo, L. Jr.

    1998-04-01

    Radionuclide ({sup 3}H, {sup 90}Sr, {sup 137}Cs, {sup 238}Pu, {sup 239}Pu, and total uranium) and heavy metal (Ag, As, Ba, Be, Cd, Cr, Hg, Ni, Pb, Sb, Se, and TI) concentrations were determined in rainbow trout collected from Tsichomo, Nana Ka, Wen Povi, and Pin De lakes in Santa Clara Canyon in 1997. Most radionuclide and heavy metal concentrations in fish collected from these four lakes were within or just above upper limit background concentrations (Abiquiu reservoir), and as a group were statistically (p < 0.05) similar in most parameters to background.

  5. CARACTERIZACIÓN DE LA ACCIDENTALIDAD VEHICULAR Y ANÁLISIS DE LAS CAUSAS EN LA PROVINCIA DE VILLA CLARA, CUBA

    OpenAIRE

    García Depestre, René A; Domingo E. Delgado Martínez; Eduardo E. Díaz García; GARCÍA ARMENTEROS, RENÉ R.

    2012-01-01

    La investigación que se presenta tiene como objeto de estudio las carreteras rurales de interés nacional en la provincia de Villa Clara, localizada en la región central de Cuba, con el propósito de analizar la seguridad vial en los tramos que componen dicha red. Se realiza la caracterización de la accidentalidad vehicular, mediante un procedimiento que contempla cinco aspectos e incluye la determinación de un orden de peligrosidad. Además, se determina la influencia de cada uno de los element...

  6. Analysis of the stormwater drainage of the historic walls of Cartagena de Indias between the bastions of San Lucas, Santa Catalina and Santa Clara

    Science.gov (United States)

    Utria, A.; Saba, M.; Quiñones-Bolaños, E.

    2017-12-01

    The city walls of Cartagena between the Baluarte of San Lucas and Santa Clara reflects the consequences of poor stormwater drainage. In the present investigation the current drainage state of this area have been evaluated, assessing its sizing and conditions to be then modelled through SWMM 5 software for return periods of 5, 10, 15 and 20 years respectively. Finally, it has been determined that the physical deterioration of the drainage elements justifies the malfunction of the whole system, therefore cleaning and periodic maintenance of the drainage elements is strongly suggested.

  7. The Effect of Stimulative Substance on the Content of Components in Cow´s Milk and the Number of Ciliates in Rumen Fluid

    Directory of Open Access Journals (Sweden)

    Jana Hnisová

    2012-10-01

    Full Text Available The aim of this work was to assess the impact of the liquid Biopolym FZT on quality components in cow´s milk and the number of microorganisms, respectively ciliates in the rumen fluid. Biopolym was, calibrated by a milking robot, given to dairy cows in a selected breeding in South Bohemian region for a selected period of time. The constituents of milk, there was a slight increase in both values in milk fat, and protein values. The number of ciliates in 1 ml of rumen fluid was higher in the experimental group (241 000 than in the control group (130 000.

  8. Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans

    OpenAIRE

    Yin, Fei; Qian, Dong

    2017-01-01

    Background False kelpfish (Sebastiscus marmoratus) is one of the target species in artificial breeding in China, and is susceptible to infection by Cryptocaryon irritans, which is an obligate parasitic ciliate that lives in the epithelium of the fish gills, skin and fins. Here, we sought to understand the mechanisms of molecular immunity of S. marmoratus against C. irritans infection. Methods We carried out an extensive analysis of the transcriptome of S. marmoratus immune-related tissues. A ...

  9. [Morphological variations of the nuclear apparatus of astome ciliates Almophrya bivacuolata and A. maediovacuolata (protozoa: ciliophora) endocommensal of terricolous oligochaetes in Cameroon].

    Science.gov (United States)

    Nana, P A; Ngassam, P; Fokam, Z; Bricheux, G; Bouchard, P; Coffe, G; Sime, Gando T; Zébazé, Togouet S H

    2010-12-01

    The silver impregnation supplemented by DAPI and Feulgen nuclear coloration enabled us to study the morphological variations of the nuclear apparatus of two species of endocommensal Astome ciliates, Almophrya bivacuoloata (de Puytorac & Dragesco, 1968) and A. mediovocuolata (Ngassam, 1983). We highlighted important digitations and the presence of dark bands in the structure of the "H" macronucleus of the small cellular types as well as the presence of intermediate forms between "H" and "X" in these two species.

  10. Morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia

    Energy Technology Data Exchange (ETDEWEB)

    Jumat, Muhammad Raihan [School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); Yan, Yan [Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228 (Singapore); Ravi, Laxmi Iyer [School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); Wong, Puisan [Detection and Diagnostics Laboratory, DSO National Laboratories, 27 Medical Drive, Singapore 117510 (Singapore); Huong, Tra Nguyen [School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); Li, Chunwei [Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228 (Singapore); Tan, Boon Huan [Detection and Diagnostics Laboratory, DSO National Laboratories, 27 Medical Drive, Singapore 117510 (Singapore); Wang, De Yun [Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228 (Singapore); Sugrue, Richard J., E-mail: rjsugrue@ntu.edu.sg [School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore)

    2015-10-15

    The distribution of cilia and the respiratory syncytial virus (RSV) nucleocapsid (N) protein, fusion (F) protein, attachment (G) protein, and M2-1 protein in human ciliated nasal epithelial cells was examined at between 1 and 5 days post-infection (dpi). All virus structural proteins were localized at cell surface projections that were distinct from cilia. The F protein was also trafficked into the cilia, and while its presence increased as the infection proceeded, the N protein was not detected in the cilia at any time of infection. The presence of the F protein in the cilia correlated with cellular changes in the cilia and reduced cilia function. At 5 dpi extensive cilia loss and further reduced cilia function was noted. These data suggested that although RSV morphogenesis occurs at non-cilia locations on ciliated nasal epithelial cells, RSV infection induces changes in the cilia body that leads to extensive cilia loss. - Highlights: • Respiratory syncytial virus (RSV) infects nasal ciliated epithelial cells. • Virus morphogenesis occurs within filamentous projections distinct from cilia. • The RSV N protein was not detected in the cilia at any time during infection. • Trafficking of the F protein into the cilia occurred early in infection. • Presence of the F protein in cilia correlated with impaired cilia function.

  11. Can a fermentation gas mainly produced by rumen Isotrichidae ciliates be a potential source of biohydrogen and a fuel for a chemical fuel cell?

    Science.gov (United States)

    Piela, Piotr; Michałowski, Tadeusz; Miltko, Renata; Szewczyk, Krzysztof; Sikora, Radosław; Grzesiuk, Elzbieta; Sikora, Anna

    2010-07-01

    Bacteria, fungi and protozoa inhabiting the rumen, the largest chamber of the ruminants' stomach, release large quantities of hydrogen during the fermentation of carbohydrates. The hydrogen is used by coexisting methanogens to produce methane in energy-yielding processes. This work shows, for the first time, a fundamental possibility of using a hydrogen-rich fermentation gas produced by selected rumen ciliates to feed a low-temperature hydrogen fuel cell. A biohydrogen fuel cell (BHFC) was constructed consisting of (i) a bioreactor, in which a hydrogen-rich gas was produced from glucose by rumen ciliates, mainly of the Isotrichidae family, deprived of intra- and extracellular bacteria, methanogens, and fungi, and (ii) a chemical fuel cell of the polymer-electrolyte type (PEFC). The fuel cell was used as a tester of the technical applicability of the fermentation gas produced by the rumen ciliates for power generation. The average estimated hydrogen yield was ca. 1.15 mol H2 per mol of fermented glucose. The BHFC performance was equal to the performance of the PEFC running on pure hydrogen. No fuel cell poisoning effects were detected. A maximum power density of 1.66 kW/m2 (PEFC geometric area) was obtained at room temperature. The maximum volumetric power density was 128 W/m3 but the coulombic efficiency was only ca. 3.8%. The configuration of the bioreactor limited the continuous operation time of this BHFC to ca. 14 hours.

  12. CARACTERIZACIÓN DE LA ACCIDENTALIDAD VEHICULAR Y ANÁLISIS DE LAS CAUSAS EN LA PROVINCIA DE VILLA CLARA, CUBA

    Directory of Open Access Journals (Sweden)

    RENÉ A. GARCÍA DEPESTRE

    2012-01-01

    Full Text Available La investigación que se presenta tiene como objeto de estudio las carreteras rurales de interés nacional en la provincia de Villa Clara, localizada en la región central de Cuba, con el propósito de analizar la seguridad vial en los tramos que componen dicha red. Se realiza la caracterización de la accidentalidad vehicular, mediante un procedimiento que contempla cinco aspectos e incluye la determinación de un orden de peligrosidad. Además, se determina la influencia de cada uno de los elementos componentes del sistema de seguridad vial en la accidentalidad y su interrelación. El área de estudio es la provincia de Villa Clara, para la cual se particulariza en el aporte a la inseguridad vial, de los elementos relativos a la carretera, a partir del análisis de los modelos de reportes de accidentes de tránsito en tres momentos, contribuyendo de esta forma con la propuesta medidas, regulaciones o acciones de conservación a favor de la disminución de los accidentes de tránsito, como resultado de un mayor conocimiento de sus causas.

  13. Education and counseling of pregnant patients with chronic hepatitis B: perspectives from obstetricians and perinatal nurses in Santa Clara County, California.

    Science.gov (United States)

    Yang, Elizabeth J; Cheung, Chrissy M; So, Samuel K S; Chang, Ellen T; Chao, Stephanie D

    2013-01-01

    This study aimed to better understand the barriers to perinatal hepatitis B prevention and to identify the reasons for poor hepatitis B knowledge and delivery of education to hepatitis B surface-antigen- positive pregnant women among healthcare providers in Santa Clara County, California. Qualitative interviews were conducted with 16 obstetricians and 17 perinatal nurses in Santa Clara County, California, which has one of the largest populations in the United States at high risk for perinatal hepatitis B transmission. Most providers displayed a lack of self-efficacy attributed to insufficient hepatitis B training and education. They felt discouraged from counseling and educating their patients because of a lack of resources and discouraging patient attitudes such as stigma and apathy. Providers called for institutional changes from the government, hospitals, and nonprofit organizations to improve care for patients with chronic hepatitis B. Early and continuing provider training, increased public awareness, and development of comprehensive resources and new programs may contribute to reducing the barriers for health care professionals to provide counseling and education to pregnant patients with chronic hepatitis B infection.

  14. Estudio de la resistencia a compresión simple del adobe elaborado con suelos procedentes de Crescencio Valdés, Villa Clara, Cuba

    Directory of Open Access Journals (Sweden)

    Barroso, I. J.

    2008-09-01

    Full Text Available In this article we study the resistance to simple compression of the adobe bricks with building purposes. The study was carried out with various clay soils from Crescencio Valdés village, municipality of Camajuaní, Villa Clara, Cuba. In order to select the best soils to construction we have considered 1 MPa (10 kg/cm2 as minimum resistance to simple compression. So the soils that do not verify that requirement have been improved only by adding sand.En este artículo se estudia la resistencia a compresión simple que presenta el adobe elaborado con diferentes suelos procedentes del pueblo de Crescencio Valdés, situado en el municipio de Camajuaní, Villa Clara, Cuba. Se valora la idoneidad de la utilización de cada suelo estudiado bajo la premisa de alcanzar con el adobe elaborado una resistencia a compresión simple de 1 MPa (10 kg/cm2, ya que dicho adobe será utilizado posteriormente para edificación en dicho pueblo

  15. Tem orixá no samba: Clara Nunes e a presença do candomblé e da umbanda na música popular brasileira

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    Rachel Rua Baptista Bakke

    2007-12-01

    Full Text Available Algumas informações sobre as religiões afro-brasileiras (candomblé e umbanda nos chegam por meios como a indústria fonográfica, o rádio e a televisão. Nesse cenário, a música popular brasileira (MPB ocupa importante papel de divulgadora dessa religiosidade. Em vista disso, nesse trabalho procuro interpretar os modos pelos quais os valores dessas religiões aparecem na MPB tendo como campo empírico a produção artística de Clara Nunes (análise de letra de música, da performance em shows, clipes e apresentações em programas de TV, assim como dos símbolos escolhidos na divulgação de seu trabalho, presentes em encartes e capas de LPs.Information about Afro-Brazilian religions (candomblé and umbanda often reaches the general public through the phonographic industry, radio and television. Brazilian Popular Music (MPB is also an important source of information about these religions. For this reason this paper attempts to interpret the ways in which the values from these religions appear on MPB, having as an empirical field the artistic production of Clara Nunes (analyzes of lyrics, performances in concerts, video clips and appearances in TV shows, as well as the symbols chosen to disseminate her work, shown in LP covers and inner-sleeves.

  16. Ground-Water Quality Data in the Santa Clara River Valley Study Unit, 2007: Results from the California GAMA Program

    Science.gov (United States)

    Montrella, Joseph; Belitz, Kenneth

    2009-01-01

    Ground-water quality in the approximately 460-square-mile Santa Clara River Valley study unit (SCRV) was investigated from April to June 2007 as part of the statewide Priority Basin project of the Ground-Water Ambient Monitoring and Assessment (GAMA) Program. The GAMA Priority Basin project was developed in response to the Groundwater Quality Monitoring Act of 2001 and is being conducted by the U.S. Geological Survey (USGS) in cooperation with the California State Water Resources Control Board (SWRCB). The study was designed to provide a spatially unbiased assessment of the quality of raw ground water used for public water supplies within SCRV, and to facilitate a statistically consistent basis for comparing water quality throughout California. Fifty-seven ground-water samples were collected from 53 wells in Ventura and Los Angeles Counties. Forty-two wells were selected using a randomized grid-based method to provide statistical representation of the study area (grid wells). Eleven wells (understanding wells) were selected to further evaluate water chemistry in particular parts of the study area, and four depth-dependent ground-water samples were collected from one of the eleven understanding wells to help understand the relation between water chemistry and depth. The ground-water samples were analyzed for a large number of synthetic organic constituents (volatile organic compounds [VOC], pesticides and pesticide degradates, potential wastewater-indicator compounds, and pharmaceutical compounds), a constituent of special interest (perchlorate), naturally occurring inorganic constituents (nutrients, major and minor ions, and trace elements), radioactive constituents, and microbial constituents. Naturally occurring isotopes (tritium, carbon-13, carbon-14 [abundance], stable isotopes of hydrogen and oxygen in water, stable isotopes of nitrogen and oxygen in nitrate, chlorine-37, and bromine-81), and dissolved noble gases also were measured to help identify the source

  17. Detecting in situ copepod diet diversity using molecular technique: development of a copepod/symbiotic ciliate-excluding eukaryote-inclusive PCR protocol.

    Science.gov (United States)

    Hu, Simin; Guo, Zhiling; Li, Tao; Carpenter, Edward J; Liu, Sheng; Lin, Senjie

    2014-01-01

    Knowledge of in situ copepod diet diversity is crucial for accurately describing pelagic food web structure but is challenging to achieve due to lack of an easily applicable methodology. To enable analysis with whole copepod-derived DNAs, we developed a copepod-excluding 18S rDNA-based PCR protocol. Although it is effective in depressing amplification of copepod 18S rDNA, its applicability to detect diverse eukaryotes in both mono- and mixed-species has not been demonstrated. Besides, the protocol suffers from the problem that sequences from symbiotic ciliates are overrepresented in the retrieved 18S rDNA libraries. In this study, we designed a blocking primer to make a combined primer set (copepod/symbiotic ciliate-excluding eukaryote-common: CEEC) to depress PCR amplification of symbiotic ciliate sequences while maximizing the range of eukaryotes amplified. We firstly examined the specificity and efficacy of CEEC by PCR-amplifying DNAs from 16 copepod species, 37 representative organisms that are potential prey of copepods and a natural microplankton sample, and then evaluated the efficiency in reconstructing diet composition by detecting the food of both lab-reared and field-collected copepods. Our results showed that the CEEC primer set can successfully amplify 18S rDNA from a wide range of isolated species and mixed-species samples while depressing amplification of that from copepod and targeted symbiotic ciliate, indicating the universality of CEEC in specifically detecting prey of copepods. All the predetermined food offered to copepods in the laboratory were successfully retrieved, suggesting that the CEEC-based protocol can accurately reconstruct the diets of copepods without interference of copepods and their associated ciliates present in the DNA samples. Our initial application to analyzing the food composition of field-collected copepods uncovered diverse prey species, including those currently known, and those that are unsuspected, as copepod prey

  18. Detecting in situ copepod diet diversity using molecular technique: development of a copepod/symbiotic ciliate-excluding eukaryote-inclusive PCR protocol.

    Directory of Open Access Journals (Sweden)

    Simin Hu

    Full Text Available Knowledge of in situ copepod diet diversity is crucial for accurately describing pelagic food web structure but is challenging to achieve due to lack of an easily applicable methodology. To enable analysis with whole copepod-derived DNAs, we developed a copepod-excluding 18S rDNA-based PCR protocol. Although it is effective in depressing amplification of copepod 18S rDNA, its applicability to detect diverse eukaryotes in both mono- and mixed-species has not been demonstrated. Besides, the protocol suffers from the problem that sequences from symbiotic ciliates are overrepresented in the retrieved 18S rDNA libraries. In this study, we designed a blocking primer to make a combined primer set (copepod/symbiotic ciliate-excluding eukaryote-common: CEEC to depress PCR amplification of symbiotic ciliate sequences while maximizing the range of eukaryotes amplified. We firstly examined the specificity and efficacy of CEEC by PCR-amplifying DNAs from 16 copepod species, 37 representative organisms that are potential prey of copepods and a natural microplankton sample, and then evaluated the efficiency in reconstructing diet composition by detecting the food of both lab-reared and field-collected copepods. Our results showed that the CEEC primer set can successfully amplify 18S rDNA from a wide range of isolated species and mixed-species samples while depressing amplification of that from copepod and targeted symbiotic ciliate, indicating the universality of CEEC in specifically detecting prey of copepods. All the predetermined food offered to copepods in the laboratory were successfully retrieved, suggesting that the CEEC-based protocol can accurately reconstruct the diets of copepods without interference of copepods and their associated ciliates present in the DNA samples. Our initial application to analyzing the food composition of field-collected copepods uncovered diverse prey species, including those currently known, and those that are unsuspected

  19. Usual and unusual biochemical properties of ADF/cofilin-like protein Adf73p in ciliate Tetrahymena thermophila.

    Science.gov (United States)

    Shiozaki, Nanami; Nakano, Kentaro; Takaine, Masak; Abe, Hiroshi; Numata, Osamu

    2009-12-04

    Actin-depolymerizing factor (ADF)/cofilin is a well-conserved actin-modulating protein, which induces reorganization of the actin cytoskeleton by severing and depolymerizing F-actin. ADF/cofilin also binds to G-actin and inhibits nucleotide exchange, and hence, is supposed to regulate the nucleotide-bound state of the cellular G-actin pool cooperating with profilin, another well-conserved G-actin-binding protein that promotes nucleotide exchange. In this report, we investigated the biochemical properties of the ADF/cofilin-like protein Adf73p from ciliate Tetrahymena thermophila. Adf73p also binds to both G- and F-actin and severs and depolymerizes F-actin. Unlike canonical ADF/cofilin, however, Adf73p accelerates nucleotide exchange on actin and allows repolymerization of disassembled actin. These results suggest that the actin cytoskeleton of T. thermophila is regulated by Adf73p in a different way from those of mammals, plants, and yeasts.

  20. Tissue mortality by Caribbean ciliate infection and white band disease in three reef-building coral species

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    Alejandra Verde

    2016-07-01

    Full Text Available Caribbean ciliate infection (CCI and white band disease (WBD are diseases that affect a multitude of coral hosts and are associated with rapid rates of tissue losses, thus contributing to declining coral cover in Caribbean reefs. In this study we compared tissue mortality rates associated to CCI in three species of corals with different growth forms: Orbicella faveolata (massive-boulder, O. annularis (massive-columnar and Acropora cervicornis (branching. We also compared mortality rates in colonies of A. cervicornis bearing WBD and CCI. The study was conducted at two locations in Los Roques Archipelago National Park between April 2012 and March 2013. In A. cervicornis, the rate of tissue loss was similar between WBD (0.8 ± 1 mm/day, mean ± SD and CCI (0.7 ± 0.9 mm/day. However, mortality rate by CCI in A. cervicornis was faster than in the massive species O. faveolata (0.5 ± 0.6 mm/day and O. annularis (0.3 ± 0.3 mm/day. Tissue regeneration was at least fifteen times slower than the mortality rates for both diseases regardless of coral species. This is the first study providing coral tissue mortality and regeneration rates associated to CCI in colonies with massive morphologies, and it highlights the risks of further cover losses of the three most important reef-building species in the Caribbean.

  1. Morphology and Phylogeny of the Soil Ciliate Metopus yantaiensis n. sp. (Ciliophora, Metopida), with Identification of the Intracellular Bacteria.

    Science.gov (United States)

    Omar, Atef; Zhang, Qianqian; Zou, Songbao; Gong, Jun

    2017-11-01

    The morphology and infraciliature of a new ciliate, Metopus yantaiensis n. sp., discovered in coastal soil of northern China, were investigated. It is distinguished from its congeners by a combination of the following features: nuclear apparatus situated in the preoral dome; 18-21 somatic ciliary rows, of which three extend onto the preoral dome (dome kineties); three to five distinctly elongated caudal cilia, and 21-29 adoral polykinetids. The 18S rRNA genes of this new species and two congeners, Metopus contortus and Metopus hasei, were sequenced and phylogenetically analyzed. The new species is more closely related to M. hasei and the clevelandellids than to other congeners; both the genus Metopus and the order Metopida are not monophyletic. In addition, the digestion-resistant bacteria in the cytoplasm of M. yantaiensis were identified, using a 16S rRNA gene clone library, sequencing, and fluorescence in situ hybridization. The detected intracellular bacteria are affiliated with Sphingomonadales, Rhizobiales, Rickettsiales (Alphaproteobacteria), Pseudomonas (Gammaproteobacteria), Rhodocyclales (Betaproteobacteria), Clostridiales (Firmicutes), and Flavobacteriales (Bacteroidetes). © 2017 The Author(s) Journal of Eukaryotic Microbiology © 2017 International Society of Protistologists.

  2. Infraciliature and morphogenesis in three rumen Diplodinium ciliates, Diplodinium polygonale, Diplodinium leche, and Diplodinium nanum, observed by light microscopy.

    Science.gov (United States)

    Ito, Akira; Imai, Soichi

    2005-01-01

    Infraciliature and morphogenesis of three rumen ophryoscolecid ciliates, Diplodinium polygonale Dogiel, 1925, Diplodinium leche Imai et al., 1992, and Diplodinium nanum Imai, 1988, are described from pyridinated silver carbonate-impregnated specimens. These three species have two polybrachykineties in the buccal area and a polybrachykinety in the dorsal ciliary zone. The vestibular polybrachykinety (VP) of D. polygonale and D. leche arises from the dorsal extremity of the adoral polybrachykinety (AP) as in Entodinium species, extending toward the left in D. polygonale and toward the left posterior in D. leche. The VP of D. nanum arises from the inner side of the AP, separate from its dorsal extremity, as in other Diplodinium species and extends toward the left posterior. These series of the polybrachykinety arrangements in D. polygonale, D. leche, and D. nanum can be regarded as transitional forms in the evolution of an Entodinium-like ancestor to Diplodinium. Morphogenesis of these three Diplodinium species is not different from that of other Diplodinium species.

  3. Transduction efficiencies of novel AAV vectors in mouse airway epithelium in vivo and human ciliated airway epithelium in vitro.

    Science.gov (United States)

    Limberis, Maria P; Vandenberghe, Luk H; Zhang, Liqun; Pickles, Raymond J; Wilson, James M

    2009-02-01

    We have characterized the ability of adeno-associated virus (AAV) serotypes 1-9 in addition to nineteen novel vectors isolated from various tissues, to transduce mouse and human ciliated airway epithelium (HAE). Vectors expressing alpha-1-antitrypsin (AAT) and beta-galactosidase were co-instilled into the mouse lung. Of all the vectors tested rh.64R1, AAV5 and AAV6 were the most efficient. The high transduction observed in mouse was reproduced in HAE cell cultures for both rh.64R1 and AAV6 but not for AAV5. Since AAV6 was the most efficient vector in mouse and HAE we also tested the transduction efficiencies of the AAV6 singleton vectors (i.e., AAV6 variants with targeted mutations) in these models. Of these, AAV6.2 transduced mouse airway epithelium and HAE with greater efficiency than all other AAV vectors tested. We demonstrated that AAV6.2 exhibits improved transduction efficiency compared to previously reported AAVs in mouse airways and in culture models of human airway epithelium and that this vector requires further development for preclinical and clinical testing.

  4. Morphologic and Genetic Variability in the Marine Planktonic Ciliate Laboea strobila Lohmann, 1908 (Ciliophora, Oligotrichia), with Notes on its Ontogenesis

    Science.gov (United States)

    AGATHA, SABINE; STRÜDER-KYPKE, MICHAELA C.; BERAN, ALFRED

    2010-01-01

    Laboea strobila Lohmann, 1908 is a conspicuous oligotrich ciliate in the marine plankton. In order to compare different populations, the morphology of specimens from the Mediterranean Sea, North Sea, and Irish Sea was investigated using live observation, protargol impregnation, and scanning electron microscopy. Furthermore, the PCR-amplified products of the SSrRNA gene from a monoclonal culture of L. strobila from the Mediterranean Sea were sequenced and aligned with sequences of other oligotrichs, including a population of L. strobila from the Atlantic coast of the USA. Finally, the data from the ecological literature were summarized and the cultivation methods were described. The SSrRNA gene sequences of the two distantly located L. strobila populations from the North Atlantic are identical. Likewise, the morphometrics of most populations so far investigated after protargol impregnation (i.e. from the North Atlantic) do not show obvious differences. In all computed phylogenetic trees, L. strobila groups with Strombidium species, forming a monophyletic taxon corresponding to the subclass Oligotrichia. These results are corroborated by the ontogenetic comparison. Since no type species was fixed for Laboea Lohmann, 1908, L. strobila was designated in the present paper. PMID:15218695

  5. [Study of the positional relationship of nucleolar chromatin and nucleolar compartments in somatic nuclei OPF the ciliate Didinium nasutum].

    Science.gov (United States)

    Leonova, O G; Karadzhian, B P; Ivlev, Iu F; Ivanova, Iu L; Popenko, V I

    2012-01-01

    We showed earlier that nucleoli in interphase ciliates Didinium nasutum, appearing on single ultrathin sections as individual structures, actually are parts of more complex network-like structures in which fibrillar component is located on periphery, and granular--in the central part of a nucleolus. It is known, that nucleolar organizers in D. nasutum are represented by chromatin bodies connected with nucleoli. In this work we used 3D reconstruction on the basis of serial ultrathin sections to study localization of chromatin bodies which by morphological criteria might correspond to nucleolar organizers. Our data showed, that all such chromatin bodies settled down outside of nucleoli, near the periphery of fibrillar component. Even those chromatin bodies which on single sections looked completely surrounded by fibrillar nucleolar component, actually settled down in fibrillar component cavities open to nucleoplasm. Analysis of distribution of nucleolar chromatin bodies allowed us to conclude that activity in different parts of interphase complex network-like nucleoli of D. nasutum is approximately the same.

  6. Primary and secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliate Paramecium tetraurelia

    Science.gov (United States)

    Carradec, Quentin; Götz, Ulrike; Arnaiz, Olivier; Pouch, Juliette; Simon, Martin; Meyer, Eric; Marker, Simone

    2015-01-01

    In various organisms, an efficient RNAi response can be triggered by feeding cells with bacteria producing double-stranded RNA (dsRNA) against an endogenous gene. However, the detailed mechanisms and natural functions of this pathway are not well understood in most cases. Here, we studied siRNA biogenesis from exogenous RNA and its genetic overlap with endogenous RNAi in the ciliate Paramecium tetraurelia by high-throughput sequencing. Using wild-type and mutant strains deficient for dsRNA feeding we found that high levels of primary siRNAs of both strands are processed from the ingested dsRNA trigger by the Dicer Dcr1, the RNA-dependent RNA polymerases Rdr1 and Rdr2 and other factors. We further show that this induces the synthesis of secondary siRNAs spreading along the entire endogenous mRNA, demonstrating the occurrence of both 3′-to-5′ and 5′-to-3′ transitivity for the first time in the SAR clade of eukaryotes (Stramenopiles, Alveolates, Rhizaria). Secondary siRNAs depend on Rdr2 and show a strong antisense bias; they are produced at much lower levels than primary siRNAs and hardly contribute to RNAi efficiency. We further provide evidence that the Paramecium RNAi machinery also processes single-stranded RNAs from its bacterial food, broadening the possible natural functions of exogenously induced RNAi in this organism. PMID:25593325

  7. Temperature-dependent transmission and latency of Holospora undulata, a micronucleus-specific parasite of the ciliate Paramecium caudatum.

    Science.gov (United States)

    Fels, Daniel; Kaltz, Oliver

    2006-04-22

    Transmission of parasites to new hosts crucially depends on the timing of production of transmission stages and their capacity to start an infection. These parameters may be influenced by genetic factors, but also by the environment. We tested the effects of temperature and host genotype on infection probability and latency in experimental populations of the ciliate Paramecium caudatum, after exposure to infectious forms of its bacterial parasite Holospora undulata. Temperature had a significant effect on the expression of genetic variation for transmission and maintenance of infection. Overall, low temperature (10 degrees C) increased levels of (multiple) infection, but arrested parasite development; higher temperatures (23 and 30 degrees C) accelerated the onset of production of infectious forms, but limited transmission success. Viability of infectious forms declined rapidly at 23 and 30 degrees C, thereby narrowing the time window for transmission. Thus, environmental conditions can generate trade-offs between transmission relevant parameters and alter levels of multiple infection or parasite-mediated selection, which may affect evolutionary trajectories of parasite life history or virulence.

  8. Thousands of RNA-cached copies of whole chromosomes are present in the ciliate Oxytricha during development.

    Science.gov (United States)

    Lindblad, Kelsi A; Bracht, John R; Williams, April E; Landweber, Laura F

    2017-08-01

    The ciliate Oxytricha trifallax maintains two genomes: a germline genome that is active only during sexual conjugation and a transcriptionally active, somatic genome that derives from the germline via extensive sequence reduction and rearrangement. Previously, we found that long noncoding (lnc) RNA "templates"-telomere-containing, RNA-cached copies of mature chromosomes-provide the information to program the rearrangement process. Here we used a modified RNA-seq approach to conduct the first genome-wide search for endogenous, telomere-to-telomere RNA transcripts. We find that during development, Oxytricha produces long noncoding RNA copies for over 10,000 of its 16,000 somatic chromosomes, consistent with a model in which Oxytricha transmits an RNA-cached copy of its somatic genome to the sexual progeny. Both the primary sequence and expression profile of a somatic chromosome influence the temporal distribution and abundance of individual template RNAs. This suggests that Oxytricha may undergo multiple rounds of DNA rearrangement during development. These observations implicate a complex set of thousands of long RNA molecules in the wiring and maintenance of a highly elaborate somatic genome architecture. © 2017 Lindblad et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  9. Un monestir a la cruïlla. Els inventaris de sagristia del monestir de Sant Antoni i Santa Clara de Barcelona (1389-1461

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    Jornet Benito, Núria

    2014-06-01

    Full Text Available The Monastery of Santa Clara and San Antonio in Barcelona was the first community of the Order of St. Clare in Catalonia. The present article reveals the wealth of its sacristy inventories as first-class historical sources. The study illustrates the special characteristics of the series of documents in the monastery’s archives: its authorship (by female sacristans as well as the main types of items inventoried and described (liturgical clothing and ornaments, jewelry, works of art, books .... The ensemble provides a rich perspective on female monastic culture with regard to its involvement in liturgy, sacred imagery and written culture. One of the outstanding results is an appreciation of the careful and detailed functional design applied by the community of nuns to each item, to keep track of time and the liturgical calendar, as well as to manage and live their relationship with the outside world.[ca] En el present article es posa al descobert la riquesa com a font històrica de primer ordre dels inventaris de sagristia del monestir de Sant Antoni i Santa Clara de Barcelona, primera comunitat de l’orde de Santa Clara en terres catalanes. L’estudi ens acosta a les particularitats d’aquesta sèrie documental en el seu fons arxivístic, la seva autoria (el càrrec de sagristana, així com a les principals tipologies de béns inventariats i descrits (parament i vestimenta litúrgica, orfebreria, obres d’art, llibres.... El conjunt permet donar una imatge rica de la cultura monàstica femenina, en l’acompanyament de la litúrgia, en la imatgeria sagrada, en la cultura escrita. Entre els resultats, sobresurt, la concepció funcional, curosa i detallada que la comunitat de germanes féu de cada peça, a l’hora de marcar el temps i el calendari litúrgic, com també a l’hora de gestionar i viure el dins/fora, la relació amb l’exterior.

  10. ANÁLISIS ESPACIAL DE LA MORBIMORTALIDAD DEL CÁNCER DE MAMAY CÉRVIX. VILLA CLARA. CUBA. 2004-2009

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    Norma E. Batista Hernández

    2013-01-01

    Full Text Available Fundamentos: El cáncer de mama y el de cérvix continúan siendo un problema de salud, a pesar de la existencia de programas de prevención y de diagnóstico precoz. Por no existir en nuestra provincia estudios similares, se decidió realizar esta investigación, con el objetivo de identificar conglomerados espaciales y/o espacio-temporales que permitan el análisis de la morbimortalidad por cáncer de mama y cérvix en la provincia Villa Clara. Métodos: Estudio observacional descriptivo, focalizado en la detección de conglomerados espaciales y espacio-temporales de la incidencia y mortalidad del cáncer de mama y cérvix en Villa Clara, utilizando la técnica estadística de exploración espacio-temporal (programa SatScan v .7.01. La muestra abarcó la totalidad de mujeres diagnosticadas durante el año 2004 y de ellas las fallecidas hasta el cierre del año 2009. Resultados: El cáncer de mama mostró concordancia en los resultados tanto espaciales como espacio-temporales con un riesgo de padecer la enfermedad de 1,63 y 1,91 respectivamente, los municipios que conformaron conglomerados significativos se ubicaron hacia el centro y noreste de la provincia, encabezados por Santa Clara, cabecera provincial. El cáncer de cérvix evidenció desde el punto de vista temporal el diagnóstico de 100 casos frente a 68,8 esperados durante los meses de marzo a julio del 2004. El estudio espacio-temporal mostró un conglomerado de incidencia en los municipios ubicados al noreste de la provincia. El riesgo relativo de presentar la enfermedad para las mujeres que vivían en estas zonas fue 3,46 frente a las del resto de la provincia. Conclusiones: Se mostró la presencia de conglomerados estadísticamente significativos tanto espaciales como espacio-temporales en las áreas de mayor riesgo de enfermar por estos cánceres. No hubo conglomerados de muertes y diagnósticos tardíos.

  11. El uso de las redes sociales en la campaña presidencial de Clara López Obregón en 2014

    OpenAIRE

    Carmona Ospina, Andrés Felipe

    2015-01-01

    El presente estudio de caso plantea la descripción detallada del uso de las redes sociales en la campaña presidencial de Clara López. En este documento se analiza la labor desempeñada en las redes sociales como un papel de interacción y comunicación directa con el electorado y su alcance en función de la estrategia comunicativa y publicitaria. Para ello se describe el contexto en el que se desarrolló la campaña, sus fases, la organización de la oficina de comunicación digital y el trabajo des...

  12. Evaluación geoambiental de la ciudad y el municipio de Santa Clara, Cuba, para el perfeccionamiento del sistema de vigilancia de salud

    Directory of Open Access Journals (Sweden)

    Mabel Font Aranda

    2008-06-01

    Full Text Available El artículo expone los resultados de la evaluación geoambiental de la ciudad y el municipio de Santa Clara, Cuba, desarrollada con el propósito de determinar y focalizar problemas de salud ambiental, y proponer acciones preventivas para atenuarlos. Con el fin de contribuir al perfeccionamiento del Sistema de Vigilancia en Salud se desarrollan los fundamentos teóricometodológicos que justifican la contribución de las ciencias geográficas a la solución del problema y se implementan en el caso de estudio, aportando una valiosa información al Sectorial Municipal de Salud Pública y a la Unidad Municipal de Higiene y Epidemiología. La misma favorece la toma de decisiones rápidas y oportunas ante factores de riesgo en su dimensión espacial y territorial.  

  13. Geo-environmental assessment of the city and municipality of Santa Clara for improving the health surveillance system Evaluación geoambiental de la ciudad y el municipio de Santa Clara, Cuba, para el perfeccionamiento del sistema de vigilancia de salud

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    Mabel Font Aranda

    2008-06-01

    Full Text Available This paper presents the results of the geo-environmental assessment of the city and municipality of Santa Clara, Cuba, developed with the purpose of determining and focusing on environmental health problems, and to propose preventive actions to minimize them. To accomplish the goal of contributing to improvement the Health Surveillance System the theoretical-methodological bases that justify the contribution of the geographical sciences to the solution of this problem have been developed. These approaches are implemented in the study case of Santa Clara, offering valuable information for the office of Municipal Public Health and the Municipal Unit of Hygiene and Epidemiology. Such results favor a rapid and opportune decision-making process in relation to risk factors with spatial and territorial dimension.El artículo expone los resultados de la evaluación geoambiental de la ciudad y el municipio de Santa Clara, Cuba, desarrollada con el propósito de determinar y focalizar problemas de salud ambiental, y proponer acciones preventivas para atenuarlos. Con el fin de contribuir al perfeccionamiento del Sistema de Vigilancia en Salud se desarrollan los fundamentos teóricometodológicos que justifican la contribución de las ciencias geográficas a la solución del problema y se implementan en el caso de estudio, aportando una valiosa información al Sectorial Municipal de Salud Pública y a la Unidad Municipal de Higiene y Epidemiología. La misma favorece la toma de decisiones rápidas y oportunas ante factores de riesgo en su dimensión espacial y territorial.  

  14. Cloning, expression and molecular characterization of a 14-3-3 gene from a parasitic ciliate, Cryptocaryon irritans.

    Science.gov (United States)

    Lin, Qianqian; Yang, Mei; Huang, Zhen; Ni, Wei; Fu, Guoliang; Guo, Guowei; Wang, Zhengchao; Huang, Xiaohong

    2013-11-08

    Cryptocaryon irritans is a parasitic ciliate and responsible for cryptocaryosis of ocean teleostean. In this paper, one gene homologous to 14-3-3 was isolated from cDNA library of C. irritans trophont/protomont stage and designated as Ci14-3-3. The full-length cDNA of the gene was 892bp with an open reading frame of 744bp, which encoded a polypeptide of 247 amino acids with a predicted molecular weight of 28.4kDa. After modification of the non-universal genetic codes, the open reading frame of Ci14-3-3 was inserted into plasmid pGEX-4T-1, transformed into Escherichia coli DH5α strain and then expressed as a glutathione S transferase fusion protein (rCi14-3-3). The result of western blot analysis showed that the rCi14-3-3 had antigenicity and the Ci14-3-3 gene in C. irritans was expressed at all stages of life cycle. The endogenous Ci14-3-3 not only distributed in cytoplasm, but also presented on the plasma membrane and the front end of cytostome in newly hatched theronts. However, when theronts were dying the protein appeared as dot-like aggregates around the nucleuses. The murine anti-rCi14-3-3 sera were capable of causing agglutination/immobilization of theronts, suggesting its potential for vaccine development. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. Fluid shear stress increases transepithelial transport of Ca(2+) in ciliated distal convoluted and connecting tubule cells.

    Science.gov (United States)

    Mohammed, Sami G; Arjona, Francisco J; Latta, Femke; Bindels, René J M; Roepman, Ronald; Hoenderop, Joost G J

    2017-05-01

    In kidney, transcellular transport of Ca(2+) is mediated by transient receptor potential vanilloid 5 and Na(+)-Ca(2+) exchanger 1 proteins in distal convoluted and connecting tubules (DCTs and CNTs, respectively). It is not yet understood how DCT/CNT cells can adapt to differences in tubular flow rate and, consequently, Ca(2+) load. This study aims to elucidate the molecular mechanisms by which DCT/CNT cells sense fluid dynamics to control transepithelial Ca(2+) reabsorption and whether their primary cilia play an active role in this process. Mouse primary DCT/CNT cultures were subjected to a physiologic fluid shear stress (FSS) of 0.12 dyn/cm(2) Transient receptor potential vanilloid 5 and Na(+)-Ca(2+) exchanger 1 mRNA levels were significantly increased upon FSS exposure compared with static controls. Functional studies with (45)Ca(2+) demonstrated a significant stimulation of transepithelial Ca(2+) transport under FSS compared with static conditions. Primary cilia removal decreased Ca(2+) transport in both static and FSS conditions, a finding that correlated with decreased expression of genes involved in transepithelial Ca(2+) transport; however, FSS-induced stimulation of Ca(2+) transport was still observed. These results indicate that nephron DCT and CNT segments translate FSS into a physiologic response that implicates an increased Ca(2+) reabsorption. Moreover, primary cilia influence transepithelial Ca(2+) transport in DCTs/CNTs, yet this process is not distinctly coupled to FSS sensing by these organelles.-Mohammed, S. G., Arjona, F. J., Latta, F., Bindels, R. J. M., Roepman, R., Hoenderop, J. G. J. Fluid shear stress increases transepithelial transport of Ca(2+) in ciliated distal convoluted and connecting tubule cells. © FASEB.

  16. Protist-bacteria associations: Gammaproteobacteria and Alphaproteobacteria are prevalent as digestion-resistant bacteria in ciliated protozoa

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    Jun eGong

    2016-04-01

    Full Text Available Protistan bacterivory, a microbial process involving ingestion and digestion, is ecologically important in the microbial loop in aquatic and terrestrial ecosystems. While bacterial resistance to protistan ingestion are relatively well understood, little is known about protistan digestion in which some ingested bacteria could not be digested in cells of major protistan grazers in the natural environment. Here we report the phylogenetic identities of digestion-resistant bacteria (DRB that could survive starvation and form relatively stable associations with 11 marine and one freshwater ciliate species. Using clone library and sequencing of 16S rRNA genes, we found that the protistan predators could host a high diversity of DRB, most of which represented novel bacterial taxa that have not been cultivated. The localization inside host cells, quantity, and viability of these bacteria were checked using fluorescence in situ hybridization. The DRB were affiliated with Actinobacteria, Bacteroidetes, Firmicutes, Parcubacteria (OD1, Planctomycetes, and Proteobacteria, with Gammaproteobacteria and Alphaproteobacteria being the most frequently occurring classes. The dominance of Gamma- and Alphaproteobacteria corresponds well to a previous study of Global Ocean Sampling metagenomic data showing the widespread types of bacterial type VI and IV secretion systems (T6SS and T4SS in these two taxa, suggesting a putatively significant role of secretion systems in promoting marine protist-bacteria associations. In the DRB assemblages, opportunistic bacteria such as Alteromonadaceae, Pseudoalteromonadaceae and Vibrionaceae often presented with high proportions, indicating these bacteria could evade protistan grazing thus persist and accumulate in the community, which, however, contrasts with the well-known rarity of these bacteria in nature. This begs the question whether viral lysis is significant in killing these indigestible bacteria in microbial communities

  17. Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila

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    Xiong Jie

    2009-05-01

    Full Text Available Abstract Background Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family. Results A total of 44 putative functional cytochrome P450 genes were identified and could be classified into 13 families and 21 sub-families according to standard nomenclature. The characteristics of both the conserved intron-exon organization and scaffold localization of tandem repeats within each P450 family clade suggested that the enlargement of T. thermophila P450 families probably resulted from recent separate small duplication events. Gene expression patterns of all T. thermophila P450s during three important cell physiological stages (vegetative growth, starvation and conjugation were analyzed based on EST and microarray data, and three main categories of expression patterns were postulated. Evolutionary analysis including codon usage preference, site-specific selection and gene-expression evolution patterns were investigated and the results indicated remarkable divergences among the T. thermophila P450 genes. Conclusion The characterization, expression and evolutionary analysis of T. thermophila P450 monooxygenase genes in the current study provides useful information for understanding the characteristics and diversities of the P450 genes in the Ciliophora, and provides the baseline for functional analyses of individual P450 isoforms in this model ciliate species.

  18. Mating of the stichotrichous ciliate Oxytricha trifallax induces production of a class of 27 nt small RNAs derived from the parental macronucleus.

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    Alan M Zahler

    Full Text Available Ciliated protozoans possess two types of nuclei; a transcriptionally silent micronucleus, which serves as the germ line nucleus, and a transcriptionally active macronucleus, which serves as the somatic nucleus. The macronucleus is derived from a new diploid micronucleus after mating, with epigenetic information contributed by the parental macronucleus serving to guide the formation of the new macronucleus. In the stichotrichous ciliate Oxytricha trifallax, the macronuclear DNA is highly processed to yield gene-sized nanochromosomes with telomeres at each end. Here we report that soon after mating of Oxytricha trifallax, abundant 27 nt small RNAs are produced that are not present prior to mating. We performed next generation sequencing of Oxytricha small RNAs from vegetative and mating cells. Using sequence comparisons between macronuclear and micronuclear versions of genes, we found that the 27 nt RNA class derives from the parental macronucleus, not the developing macronucleus. These small RNAs are produced equally from both strands of macronuclear nanochromosomes, but in a highly non-uniform distribution along the length of the nanochromosome, and with a particular depletion in the 30 nt telomere-proximal positions. This production of small RNAs from the parental macronucleus during macronuclear development stands in contrast to the mechanism of epigenetic control in the distantly related ciliate Tetrahymena. In that species, 28-29 nt scanRNAs are produced from the micronucleus and these micronuclear-derived RNAs serve as epigenetic controllers of macronuclear development. Unlike the Tetrahymena scanRNAs, the Oxytricha macronuclear-derived 27 mers are not modified by 2'O-methylation at their 3' ends. We propose models for the role of these "27macRNAs" in macronuclear development.

  19. A Novel Colonial Ciliate Zoothamnium ignavum sp. nov. (Ciliophora, Oligohymenophorea and Its Ectosymbiont Candidatus Navis piranensis gen. nov., sp. nov. from Shallow-Water Wood Falls.

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    Lukas Schuster

    Full Text Available Symbioses between ciliate hosts and prokaryote or unicellular eukaryote symbionts are widespread. Here, we report on a novel ciliate species within the genus Zoothamnium Bory de St. Vincent, 1824, isolated from shallow-water sunken wood in the North Adriatic Sea (Mediterranean Sea, proposed as Zoothamnium ignavum sp. nov. We found this ciliate species to be associated with a novel genus of bacteria, here proposed as "Candidatus Navis piranensis" gen. nov., sp. nov. The descriptions of host and symbiont species are based on morphological and ultrastructural studies, the SSU rRNA sequences, and in situ hybridization with symbiont-specific probes. The host is characterized by alternate microzooids on alternate branches arising from a long, common stalk with an adhesive disc. Three different types of zooids are present: microzooids with a bulgy oral side, roundish to ellipsoid macrozooids, and terminal zooids ellipsoid when dividing or bulgy when undividing. The oral ciliature of the microzooids runs 1¼ turns in a clockwise direction around the peristomial disc when viewed from inside the cell and runs into the infundibulum, where it makes another ¾ turn. The ciliature consists of a paroral membrane (haplokinety, three adoral membranelles (polykineties, and one stomatogenic kinety (germinal kinety. One circular row of barren kinetosomes is present aborally (trochal band. Phylogenetic analyses placed Z. ignavum sp. nov. within the clade II of the polyphyletic family Zoothamniidae (Oligohymenophorea. The ectosymbiont was found to occur in two different morphotypes, as rods with pointed ends and coccoid rods. It forms a monophyletic group with two uncultured Gammaproteobacteria within an unclassified group of Gammaproteobacteria, and is only distantly related to the ectosymbiont of the closely related peritrich Z. niveum (Hemprich and Ehrenberg, 1831 Ehrenberg, 1838.

  20. The mitochondrial genome sequence of the ciliate Paramecium caudatum reveals a shift in nucleotide composition and codon usage within the genus Paramecium

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    Berendonk Thomas U

    2011-05-01

    Full Text Available Abstract Background Despite the fact that the organization of the ciliate mitochondrial genome is exceptional, only few ciliate mitochondrial genomes have been sequenced until today. All ciliate mitochondrial genomes are linear. They are 40 kb to 47 kb long and contain some 50 tightly packed genes without introns. Earlier studies documented that the mitochondrial guanine + cytosine contents are very different between Paramecium tetraurelia and all studied Tetrahymena species. This raises the question of whether the high mitochondrial G+C content observed in P. tetraurelia is a characteristic property of Paramecium mtDNA, or whether it is an exception of the ciliate mitochondrial genomes known so far. To test this question, we determined the mitochondrial genome sequence of Paramecium caudatum and compared the gene content and sequence properties to the closely related P. tetraurelia. Results The guanine + cytosine content of the P. caudatum mitochondrial genome was significantly lower than that of P. tetraurelia (22.4% vs. 41.2%. This difference in the mitochondrial nucleotide composition was accompanied by significantly different codon usage patterns in both species, i.e. within P. caudatum clearly A/T ending codons dominated, whereas for P. tetraurelia the synonymous codons were more balanced with a higher number of G/C ending codons. Further analyses indicated that the nucleotide composition of most members of the genus Paramecium resembles that of P. caudatum and that the shift observed in P. tetraurelia is restricted to the P. aurelia species complex. Conclusions Surprisingly, the codon usage bias in the P. caudatum mitochondrial genome, exemplified by the effective number of codons, is more similar to the distantly related T. pyriformis and other single-celled eukaryotes such as Chlamydomonas, than to the closely related P. tetraurelia. These differences in base composition and codon usage bias were, however, not reflected in the amino

  1. The defensive function of trichocysts in Paramecium tetraurelia against metazoan predators compared with the chemical defense of two species of toxin-containing ciliates.

    Science.gov (United States)

    Buonanno, Federico; Harumoto, Terue; Ortenzi, Claudio

    2013-04-01

    The time-honored assumption about the defensive function of trichocysts in Paramecium against predators was recently verified experimentally against different species of unicellular predators. In the present study, we examined the defensive function of trichocysts against three metazoan predators, Cephalodella sp. (Rotifera), Eucypris sp. (Arthropoda), and Stenostomum sphagnetorum (Platyhelminthes). The results confirmed the defensive function of trichocysts against two of these metazoan predators (Cephalodella sp. and Eucypris sp.), while they seem ineffective against S. sphagnetorum. We also compared the defensive efficiency of the trichocysts of P. tetraurelia with that of toxin-containing extrusomes of two ciliates.

  2. Ciliates (Protozoa from dried sediments of a temporary pond from Argentina Ciliados (Protozoa de sedimentos secos de una charca temporaria de la Argentina

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    Gabriela Cristina Küppers

    2009-12-01

    Full Text Available Temporary ponds represent special environments that are inhabited by organisms adapted to changing environmental conditions. Ciliates are able to survive complete loss of water in these transient habitats through cyst formation. However, ciliates from the Neotropical region in general have been poorly studied with modern techniques. The main goal of this study is to describe the ciliates in dried sediments of a temporary pond from Buenos Aires Province, Argentina, through sampling efforts that were performed 2003-2005. Soil samples were obtained during drought phases and re wetted in laboratory to establish raw and enriched cultures. Ciliates were then studied both in vivo and after impregnation with protargol. In this study, we present 4 new records for Argentina (Gonostomum affine (Stein, 1859 Sterki, 1878, Stylonychia bifaria (Stokes, 1887 Berger, 1999, Pleurotricha lanceolata (Ehrenberg, 1835 Stein, 1859, Meseres corlissi Petz and Foissner, 1992, 1 for South America (Blepharisma americanum (Suzuki, 1954 Hirshfield, Isquith and Bhandary, 1965, and 2 for the Neotropical Realm (Gonostomum strenuum (Engelmann, 1862 Sterki, 1878, Stylonychia lemnae Ammermann and Schlegel, 1983.Los cuerpos de agua temporarios son ambientes particulares que se encuentran habitados por organismos adaptados a condiciones fluctuantes. Los ciliados son capaces de sobrevivir a la pérdida completa de agua del ambiente gracias a la formación de estructuras de resistencia. Por otra parte, los ciliados de la región Neotropical han sido poco investigados con técnicas modernas. El objetivo de este estudio es referir los ciliados que se desarrollaron a partir de los sedimentos secos de una charca temporaria de la provincia de Buenos Aires, Argentina, en la que se realizaron muestreos durante el período 2003-2005. Las muestras de suelo fueron obtenidas durante las fases de sequía y luego resuspendidas en el laboratorio para realizar cultivos naturales y enriquecidos. Los

  3. In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes.

    Science.gov (United States)

    Grønlien, Heidi K; Berg, Trond; Løvlie, Arne M

    2002-07-01

    Ciliates use phagocytosis to acquire edible particles. The polymorphic ciliate Tetrahymena vorax appears in two forms ('microstomes' and 'macrostomes'). Transformation of microstomes into macrostomes takes place in the presence of the ciliate Tetrahymena thermophila and enables the macrostome to phagocytose the latter species. The non-specific, constitutive phagocytosis in microstomes thereby changes into a specific inducible process in macrostomes. The purpose of this study was to determine whether the phagocytotic process in macrostomes is specifically aimed at catching T. thermophila. The two forms of phagocytosis represent an interesting model system for studying the mechanism whereby phagosomes are formed. The macrostomal form capture deciliated and ciliated Tetrahymena thermophila, latex beads with diameters of 20.3 and 30.0 microm and small microstomal cells. However, the macrostomes select T. thermophila as a prey when they have the opportunity to choose between deciliated T. thermophila and latex beads and between T. thermophila and microstomes. The non-selective formation of phagosomes seen in microstomes changes to a highly selective process during the transformation to macrostomes. Unlike microstomes, macrostomes do not form a closed vacuole after capturing a latex bead, indicating that mechanical stimulation by the prey does not in itself trigger phagocytosis in the macrostomal form of T. vorax. Although macrostomes captured T. thermophila in preference to microstomes, phagocytosis of microstomes started immediately following capture, indicating that the substance/molecule that triggers the formation of the phagosome is not specific for T. thermophila cells. After capturing a T. thermophila cell, the macrostomal cell, which normally swims in a forward direction, reverses direction and swims backwards for a short time before starting to rotate. Macrostomal cells did not change their swimming pattern after capturing a latex bead. We believe, therefore

  4. The mitochondrial genome sequence of the ciliate Paramecium caudatum reveals a shift in nucleotide composition and codon usage within the genus Paramecium.

    Science.gov (United States)

    Barth, Dana; Berendonk, Thomas U

    2011-05-31

    Despite the fact that the organization of the ciliate mitochondrial genome is exceptional, only few ciliate mitochondrial genomes have been sequenced until today. All ciliate mitochondrial genomes are linear. They are 40 kb to 47 kb long and contain some 50 tightly packed genes without introns. Earlier studies documented that the mitochondrial guanine + cytosine contents are very different between Paramecium tetraurelia and all studied Tetrahymena species. This raises the question of whether the high mitochondrial G+C content observed in P. tetraurelia is a characteristic property of Paramecium mtDNA, or whether it is an exception of the ciliate mitochondrial genomes known so far. To test this question, we determined the mitochondrial genome sequence of Paramecium caudatum and compared the gene content and sequence properties to the closely related P. tetraurelia. The guanine + cytosine content of the P. caudatum mitochondrial genome was significantly lower than that of P. tetraurelia (22.4% vs. 41.2%). This difference in the mitochondrial nucleotide composition was accompanied by significantly different codon usage patterns in both species, i.e. within P. caudatum clearly A/T ending codons dominated, whereas for P. tetraurelia the synonymous codons were more balanced with a higher number of G/C ending codons. Further analyses indicated that the nucleotide composition of most members of the genus Paramecium resembles that of P. caudatum and that the shift observed in P. tetraurelia is restricted to the P. aurelia species complex. Surprisingly, the codon usage bias in the P. caudatum mitochondrial genome, exemplified by the effective number of codons, is more similar to the distantly related T. pyriformis and other single-celled eukaryotes such as Chlamydomonas, than to the closely related P. tetraurelia. These differences in base composition and codon usage bias were, however, not reflected in the amino acid composition. Most probably, the observed picture is best

  5. Avaliação dos níveis de clorofila em folhas de tomateiro da cultivar Santa Clara, do mutante ‘firme’ e do híbrido F1 - DOI: 10.4025/actascibiolsci.v25i1.2110 Chlorophyll level evaluation in tomatoes leaves of Santa Clara cultivar, of ‘firme’ mutant and hybrid F1 - DOI: 10.4025/actascibiolsci.v25i1.2110

    Directory of Open Access Journals (Sweden)

    Aldo Shimoya

    2003-04-01

    Full Text Available Recentemente, na região produtora de hortaliças de Viçosa, Estado de Minas Gerais, foram identificadas plantas de tomate da cultivar Santa Clara, Lycopersicum esculentum (Solanaceae, que se mostraram potencialmente úteis em programas de melhoramento que visem aumentar a vida de prateleira de frutos. No entanto, essas plantas promissoras, denominadas de ‘mutante firme’, apresentam senescência foliar precoce e redução do tamanho médio dos frutos, quando o gene se encontra no estado homozigótico recessivo, sendo visualmente normal quando no estado heterozigótico. Assim o objetivo do presente trabalho foi avaliar os níveis de pigmentos foliares em diferentes posições da planta e em diferentes tempos de cultivo, pelos métodos descritos por Lichtenthaler (1987 e pelo índice SPAD (Soil Plant Analylitical Division Value, para verificar a ocorrência ou não de comportamento diferencial de indivíduos heterozigotos em relação aos progenitores Santa Clara e ao mutante ‘firme’. Para isso, foram realizados dois experimentos, empregando-se plantas cultivadas em vasos, submetidas a condições de casa-de-vegetação e arranjadas em delineamento inteiramente casualizado. Os resultados das análises, pelo método de Lichtenthaler (1987 mostraram que a clorofila e os pigmentos carotenóides são degradados mais precocemente no mutante ‘firme’ em relação à cultivar Santa Clara. Além do que, pelo índice SPAD observou-se que os indivíduos, portando a mutação no estado heterozigótico, e os da cultivar Santa Clara apresentaram taxas similares de degradação de clorofila em relação ao tempo de cultivo. Com isso, pode-se concluir que a mutação no estado heterozigótico, em cruzamento com Santa Clara, tem seus efeitos restaurados para esses caracteres bioquímicosRecently, in Viçosa (MG, a region of vegetable crop producing, tomatoes of Santa Clara cultivar, Lycopersicum esculentum (Solanaceae, that showed potential use for

  6. Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans under low temperature.

    Science.gov (United States)

    Yin, Fei; Sun, Peng; Wang, Jiteng; Gao, Quanxin

    2016-05-13

    Cryptocaryon irritans, a species of obligatory ciliate ectoparasite, can infect various species of marine teleost fish. Cryptocaryon irritans that fall to the seabed or aquarium bottom in winter can form "dormant tomonts" and wake up when the temperature rises the next year. Abundant studies and analyses on the dormant tomonts were carried out at the transcriptome level, in order to investigate the molecular mechanism of C. irritans tomonts entering the dormant state under low-temperature conditions. The paired-end sequencing strategy was used to better assemble the entire transcriptome de novo. All clean sequencing reads from each of the three libraries (Group A: untreated blank control; Group B: treated for 24 h at 12 °C; and Group C: developed for 24 h at 25 °C) were respectively mapped back to the transcriptome assembly using the bioinformatics software. In this study, 25,695,034, 21,944,467, and 28,722,875 paired-end clean reads were obtained respectively from the three cDNA libraries of the C. irritans tomont by Illumina paired-end sequencing technology. A total of 25,925 unique transcript fragments (unigenes) were assembled, with an average length of 839 bp. Differentially expressed genes (DEGs) were scrutinized; in Group B/A pairwise comparison, 343 genes presented differential expression, including 265 up-regulated genes and 78 down-regulated genes in Group B; in Group C/A pairwise comparison, there were 567 DEGs, including 548 up-regulated genes and 19 down-regulated genes in Group C; and in Group B/C pairwise comparison, 185 genes showed differential expression, including 145 up-regulated genes and 40 down-regulated genes in Group B. This is the first transcriptomic analytical study of the C. irritans tomonts under low temperature. It can be concluded that most of the genes required for its cell survival under low temperature, or for cell entry into a deeper dormancy state were discovered, and that they might be considered as candidate genes to

  7. Emergence of the terrestrial ciliate Colpoda cucullus from a resting cyst: rupture of the cyst wall by active expansion of an excystment vacuole.

    Science.gov (United States)

    Funadani, Ryoji; Suetomo, Yasutaka; Matsuoka, Tatsuomi

    2013-01-01

    The first sign of excysting Colpoda cucullus cells is the initiation of the pulsation of a contractile vacuole, which is then replaced by a non-pulsating vacuole (excystment vacuole) that continues to expand and finally ruptures the outermost cyst wall (ectocyst) due to inner pressure. A ciliate surrounded by flexible membranes (endocyst) thus emerges. The osmolarity of the excysting cells is estimated to be 140 mOsm L(-1) from the relationship between the frequency of contractile vacuole pulsation and the external sucrose concentration. Both the expansion of the excystment vacuole and the emergence of ciliates occurred even when the cysts were immersed in hypertonic medium. In hypotonic medium containing sodium azide (NaN3, a cytochrome c oxidase inhibitor), the contractile vacuole of vegetative cells stopped pulsating and gradually expanded, causing cells to burst. When C. cucullus was induced to encyst in a hypotonic medium containing NaN3, the expansion of the excystment vacuoles was inhibited. These results suggest that the active uptake of water may be responsible for the expansion of the excystment vacuole required for the ectocyst to rupture.

  8. The Anti-Oxidant Defense System of the Marine Polar Ciliate Euplotes nobilii: Characterization of the MsrB Gene Family

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    Francesca Ricci

    2017-01-01

    Full Text Available Organisms living in polar waters must cope with an extremely stressful environment dominated by freezing temperatures, high oxygen concentrations and UV radiation. To shed light on the genetic mechanisms on which the polar marine ciliate, Euplotes nobilii, relies to effectively cope with the oxidative stress, attention was focused on methionine sulfoxide reductases which repair proteins with oxidized methionines. A family of four structurally distinct MsrB genes, encoding enzymes specific for the reduction of the methionine-sulfoxide R-forms, were identified from a draft of the E. nobilii transcriptionally active (macronuclear genome. The En-MsrB genes are constitutively expressed to synthesize proteins markedly different in amino acid sequence, number of CXXC motifs for zinc-ion binding, and presence/absence of a cysteine residue specific for the mechanism of enzyme regeneration. The En-MsrB proteins take different localizations in the nucleus, mitochondria, cytosol and endoplasmic reticulum, ensuring a pervasive protection of all the major subcellular compartments from the oxidative damage. These observations have suggested to regard the En-MsrB gene activity as playing a central role in the genetic mechanism that enables E. nobilii and ciliates in general to live in the polar environment.

  9. Ultrastructure observation on the cells at different life history stages of Cryptocaryon irritans (Ciliophora: Prostomatea), a parasitic ciliate of marine fishes.

    Science.gov (United States)

    Ma, Rui; Ni, Bing; Fan, Xinpeng; Warren, Alan; Yin, Fei; Gu, Fukang

    2016-09-01

    Cells of Cryptocaryon irritans at different life history stages were studied using both light and electron microscopy. The characteristics of several organelles were revealed for the first time at the ultrastructural level. It was confirmed that the cytostome of trophonts, protomonts and theronts was surrounded by cilium-palp triplets rather than ciliary triplets. The nematodesmata underlying the circumoral dikinetids were single bundles, whereas these were always paired in Prorodontids. Toxicysts were present in late-stage tomonts and theronts, but were absent in trophonts and protomonts. We posited that toxicysts might play a role in infection and invasion of host-fish tissue by theronts. The adoral brosse was unlike that of any other family of the class Prostomatea based on its location and morphology. Membranous folds were present in trophonts, protomonts and theronts. These folds were longer and more highly developed in C. irritans than in exclusively free-living prostome ciliates suggesting that they might be linked to parasitism in C. irritans. Trophonts, protomonts and theronts had multiple contractile vacuoles. The basic ultrastructure of the contractile vacuole of C. irritans was similar to that of other kinetofragminophoran ciliates. They might play different roles in different stages of the life cycle since their ultrastructure varied among trophonts, protomonts and theronts.

  10. A community model of ciliate Tetrahymena and bacteria E. coli. Part 1: Individual-based models of Tetrahymena and E. coli populations

    Energy Technology Data Exchange (ETDEWEB)

    Jaworska, J.S.; Hallam, T.G.; Schultz, T.W. [Univ. of Tennessee, Knoxville, TN (United States)

    1996-03-01

    The dynamics of a microbial community consisting of a eucaryotic ciliate Tetrahymena pyriformis and procaryotic. Escherichia coli in a batch culture is explored by employing an individual-based approach. In this portion of the article, Part 1, population models are presented. Because both models are individual-based, models of individual organisms are developed prior to construction of the population models. The individual models use an energy budget method in which growth depends on energy gain from feeding and energy sinks such as maintenance and reproduction. These models are not limited by simplifying assumptions about constant yield, constant energy sinks and Monod growth kinetics as are traditional models of microbial organisms. Population models are generated from individual models by creating distinct individual types and assigning to each type the number of real individuals they represent. A population is a compilation of individual types that vary in a phase of cell cycle and physiological parameters such as filtering rate for ciliates and maximum anabolic rate for bacteria. An advantage of the developed models is that they realistically describe the growth of the individual cells feeding on resource which varies in density and composition. Part 2, the core of the project, integrates models into a dynamic microbial community and provides model analysis based upon available data.

  11. Monoculture of the ciliate protozoan Euplotes sp. (Ciliophora; Hypotrichia fed with different diets - doi: 10.4025/actascibiolsci.v35i1.11795

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    Emílio Mateus Costa Melo

    2012-12-01

    Full Text Available Ciliate protozoa of the genus Euplotes commonly appears contaminating mass cultures of rotifers but also with potential to be used as live food in the larviculture of marine fish. To obtain a monoculture of Euplotes sp., three diets were tested: 1 microalgae Nannochloropsis oculata, 2 commercial diet for rotifers Culture Selco 3000, and 3 baker’s yeast (Saccharomyces cerevisiae. The ciliates were inoculated at 10 ind. mL-¹. On day 5, protozoa densities in the groups fed the commercial diet (1,911.0 ± 248.7 ind. mL-¹ and the baker’s yeast (2,600.0 ± 195.3 ind. mL-1 did not differ, but were higher than the group fed microalgae (2.0 ± 1.4 ind. mL-1 (p -¹ than in the groups fed microalgae (3.0 ± 1.4 ind. mL-¹ or commercial diet (11,287.0 ± 1,468.0 ind. mL-¹. An exponential growth curve was observed for the protozoa fed baker’s yeast (R² = 0.992; p Euplotes sp. can result in very high densities of this protozoan.  

  12. A morphogenetic survey on ciliate plankton from a mountain lake pinpoints the necessity of lineage-specific barcode markers in microbial ecology.

    Science.gov (United States)

    Stoeck, Thorsten; Breiner, Hans-Werner; Filker, Sabine; Ostermaier, Veronika; Kammerlander, Barbara; Sonntag, Bettina

    2014-02-01

    Analyses of high-throughput environmental sequencing data have become the 'gold-standard' to address fundamental questions of microbial diversity, ecology and biogeography. Findings that emerged from sequencing are, e.g. the discovery of the extensive 'rare microbial biosphere' and its potential function as a seed-bank. Even though applied since several years, results from high-throughput environmental sequencing have hardly been validated. We assessed how well pyrosequenced amplicons [the hypervariable eukaryotic V4 region of the small subunit ribosomal RNA (SSU rRNA) gene] reflected morphotype ciliate plankton. Moreover, we assessed if amplicon sequencing had the potential to detect the annual ciliate plankton stock. In both cases, we identified significant quantitative and qualitative differences. Our study makes evident that taxon abundance distributions inferred from amplicon data are highly biased and do not mirror actual morphotype abundances at all. Potential reasons included cell losses after fixation, cryptic morphotypes, resting stages, insufficient sequence data availability of morphologically described species and the unsatisfying resolution of the V4 SSU rRNA fragment for accurate taxonomic assignments. The latter two underline the necessity of barcoding initiatives for eukaryotic microbes to better and fully exploit environmental amplicon data sets, which then will also allow studying the potential of seed-bank taxa as a buffer for environmental changes. © 2013 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

  13. Geologic map of the Palo Alto and part of the Redwood Point 7-1/2' quadrangles, San Mateo and Santa Clara counties, California

    Science.gov (United States)

    Pampeyan, Earl H.

    1993-01-01

    The Palo Alto and southern part of the Redwood Point 7-1/2' quadrangles cover an area on the San Francisco peninsula between San Francisco Bay and the Santa Cruz Mountains. San Francisquito and Los Trancos Creeks, in the southeastern part of the map area, form the boundary between San Mateo and Santa Clara Counties. The area covered by the geologic map extends from tidal and marsh lands at the edge of the bay southward across a gently sloping alluvial plain to the foothills of the northern Santa Cruz Mountains. The foothills are separated from the main mass of the mountains by two northwest-striking faults, the San Andreas and Pilarcitos, that cross the southwest corner of the map area (fig. 1). The map and adjoining areas are here divided into three structural blocks juxtaposed along these faults, adopting the scheme of Nilsen and Brabb (1979): (1) the San Francisco Bay block lying east of the San Andreas Fault Zone; (2) the Pilarcitos block lying between the San Andreas and Pilarcitos Faults; and (3) the La Honda block that includes the main mass of the Santa Cruz Mountains lying west of the Pilarcitos Fault. The west boundary of the La Honda block is the Seal Cove-San Gregorio Fault. Pre-late Pleistocene Cenozoic rocks of the foothills have been compressed into northwest-striking folds, which have been overridden by Mesozoic rocks along southwest-dipping low-angle faults. Coarse- to fine-grained upper Pleistocene and Holocene alluvial and estuarine deposits, eroded from the foothills and composing the alluvial plain, are essentially undeformed. Most of the alluvial plain, including some parts of the marsh land that borders the bay, has been covered by residential and commercial developments, and virtually all of the remaining marsh land has been diked off and used as salt evaporating ponds. The map area includes parts of the municipalities of San Carlos, Redwood City, Atherton, Woodside, Portola Valley, Menlo Park, and East Palo Alto in San Mateo County; and

  14. Growth of late Quaternary folds in southwest Santa Clara Valley, San Francisco Bay area, California: Implications of triggered slip for seismic hazard and earthquake recurrence

    Science.gov (United States)

    Hitchcock, Christopher S.; Kelson, Keith I.

    1999-05-01

    We combine analysis of geologic and geomorphic data with observations of deformation during the 1989 Loma Prieta earthquake in northern California to evaluate the contribution of aseismic triggered slip and creep processes to cumulative late Cenozoic deformation along the northeastern Santa Cruz Mountains range front. Deformed late Pleistocene alluvial fans and terraces provide evidence for localized late Quaternary uplift above range-bounding reverse faults within the southwestern Santa Clara Valley adjacent to the range front. On the basis of offset of late Quaternary surfaces, the long-term average slip rate on the primary range-bounding structure (Monte Vista fault) is estimated to be ˜0.2 mm/yr. Northeast of the range front are several discontinuous northwest-trending folds, indicated by alignment of late Pleistocene alluvial-fan apices, anomalous stream-channel convexities, and topographic and vegetation lineaments within a 3 5-km-wide, northwest-trending corridor. Subsurface geologic and geophysical data support the interpretation that the surface folds are a result of blind reverse faulting along the Cascade fault beneath the Santa Clara Valley. From stream incision rates we estimate an average uplift rate of 0.2 ± 0.05 mm/yr for the Cascade fault. Measurements of triggered and postseismic slip following the 1989 Loma Prieta earthquake, combined with estimates of the average return period for Loma Prieta type events, suggest a long-term average deformation rate along the range front of 0.25 0.4 mm/yr associated with these aseismic processes. This range of values is comparable to rates of late Quaternary deformation on the range-front faults derived from geologic and geomorphic data, and it suggests that growth of the overlying folds is at least partially the result of triggered slip and postseismic creep associated with nearby Loma Prieta type earthquakes. If this inference is correct, then the return period for independent events on the range

  15. Diagnóstico mediante técnicas de ultrasonidos del forjado de madera del refectorio del Convento de Santa Clara en Carmona (Sevilla

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    Pérez Gálvez, Filomena

    2004-04-01

    Full Text Available Ultrasonic tests are an effective technique of getting adiagnosis of timber structures in historical buildings. Thistechnique is quite valuable to undertake a restoration job. Anew methodology of analysis and diagnosis of timber roofsthrough this non destructive technique is presented.The aim of this article is to get the state of damage and thebending strenght capacity of the timber roof of the Monasteryof Santa Clara in Carmona (Seville dining´s room by usingthis technology.From the results obtained about the bending strenghtcapacity of the studied beams, it can be affirmed that thetimber roof is not able to support safely enough the new useproposed.Considering the historic and artistic importance of thistimber roof, we propose as a restoration methodology basedon hanging the roof through bolts from a reinforced concreteplatform built-in the perimeter walls. This solutionguarantees that the timber roof does not work, just adjustingthe distance between the bolts.Los métodos de ultrasonidos son una técnica eficaz para eldiagnóstico de elementos estructurales de madera enedificios antiguos. Esta técnica proporciona una gran ayudaa la hora de acometer una obra de rehabilitación. Sepresenta una metodología de análisis y diagnostico deforjados de madera mediante dicha técnica no destructiva.El objetivo de este artículo es obtener el grado de deterioro yla capacidad resistente de la madera del alfarje delrefectorio del Convento de Santa Clara en Carmona (Sevillautilizando la metodología propuesta.Por extrapolación de los resultados obtenidos sobre lacapacidad resistente de las vigas estudiadas se puedeconcluir que el conjunto del forjado no es capaz de soportarcon seguridad suficiente el nuevo uso propuesto.Teniendo en cuenta el valor histórico artístico del alfarje sepropone y aporta como solución de rehabilitación laconsistente en colgar el forjado existente mediante pernos deuna losa de hormigón armado empotrada en los

  16. Programación del riego de la papa en el “Valle del Yabú”, Santa Clara, Cuba.

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    B. Job

    2010-02-01

    Full Text Available The objective of the current research was to study the applied irrigation scheduling of the potato during two crop cycles (2009 -2009/10 in Cooperative No. 2 in the Yabú Valley of Santa Clara. Also to carry out corrections required in order to establish a scientifically based irrigation program. In the first crop cycle it was proven that the timing of irrigation application and the amount of water applied did not meet the technical criteria but was dependant on the decisions taken by persons with practical experience in relation to this activity and the programming of irrigation characterized by the application of small depths of irrigation which did not moisten below 20 cm of soil depth in a way which the irrigation interfered with the cultural and chemical processes and left the soil with a hydric deficit. In both crops tension meter were placed at 20 and 40 soil depth, taking periodic measurement of humidity and daily measurements of soil and balance water, because of this the number of irrigation was reduce during the second crop cycle.El objetivo del presente trabajo, ejecutado durante dos cosechas 2009 y 2009-10 fue estudiar la programación del riego de la papa que se realiza en la Unidad Cooperativa No.2 del “Valle del Yabú” de Santa Clara y realizar correcciones para lograr el establecimiento de una programación de riego científicamente fundamentada. En la primera cosecha, se comprobó que el momento de aplicación del riego y el volumen de agua aportado no obedecían a criterios técnicos, sino que dependían de decisiones empíricas de las personas que se relacionaban con esta actividad y la programación del riego se caracterizaba por aplicar muy pequeñas láminas de agua que no lograban humedecer 20 cm de profundidad de suelo, de modo que, el riego obstaculizaba las labores fitosanitarias y culturales y dejaba el suelo con déficit hídrico. En ambas cosechas, se colocaron tensiómetros a 20 y 40 cm de profundidad del suelo

  17. Utilização do mecanismo de transposição de peixes da Usina Hidrelétrica Santa Clara por camarões (Palaemonidae, bacia do rio Mucuri, Minas Gerais, Brasil Use of Santa Clara Power Plant fish lift by Palaemonidae shrimps, Mucuri River basin, Minas Gerais, Brazil

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    Paulo dos S. Pompeu

    2006-03-01

    Full Text Available Durante a operação do elevador para peixes da Usina Hidrelétrica Santa Clara, de novembro de 2003 a março de 2004, todos os crustáceos palaemonídeos adultos que utilizaram o mecanismo foram contados e o número de jovens estimado. Duas espécies foram registradas: Macrobrachium carcinus (Linnaeus, 1758 e Macrobrachium acanthurus (Wiegmann, 1836. A utilização do mecanismo por adultos foi bastante restrita, com apenas 185 exemplares registrados. Porém, o número de jovens de M. carcinus utilizando o elevador foi estimado em 19.120 indivíduos. Embora o mecanismo avaliado tenha permitido a passagem dos palaemonídeos para montante do barramento, ficou clara a necessidade de novos arranjos estruturais e de manejo específicos para esses animais. Essas ações se referem ao desenvolvimento de estruturas direcionadas para a sua passagem e a adoção de vertimentos programados durante o período reprodutivo para permitir o carreamento de larvas para jusante. Esses dois caminhos representam formas efetivas de manejo, imprescindíveis para a manutenção das populações deste importante componente da biota aquática.During the Santa Clara Power Plant fish lift operation, from November 2003 to March 2004, Palaemonidae adult specimens were counted and identified and estimated juveniles number. Two Palaemonidae species were recorded: Macrobrachium carcinus (Linnaeus, 1758 and Macrobrachium acanthurus (Wiegmann, 1836. The use of the fish lift by adults was restricted, since only 185 individuals were registered. However, the estimated juveniles number was 19,120 individuals. Although existing fish passage systems could be considered as an alternative for Palaemonidae migration, planned spills during the reproductive periods could allow the drift of larvae. Moreover, the constructions of specific structures for upstream migration should be considered as an alternative for the maintenance of this important component of aquatic biota.

  18. Analysis of expressed sequence tags (ESTs) and gene expression changes under different growth conditions for the ciliate Anophryoides haemophila, the causative agent of bumper car disease in the American lobster (Homarus americanus).

    Science.gov (United States)

    Acorn, Adam R; Clark, K Fraser; Jones, Sarah; Després, Béatrice M; Munro, Sarah; Cawthorn, Richard J; Greenwood, Spencer J

    2011-06-01

    The scuticociliate Anophryoides haemophila, causes bumper car disease in American lobster (Homarus americanus) in commercial holding facilities in Atlantic Canada. While the parasite has been recognized since the 1970s and much has been learned about its biology, minimal molecular characterization exists. With genome consortiums turning to model organisms like the ciliates Tetrahymena and Paramecium, the amount of relevant sequence data available has made sequence surveys more attractive for gene discovery in related ciliates. We sequenced 9984 expressed sequence tags (ESTs) from a non-normalized A. haemophila cDNA library to characterize gene expression patterns, functional gene distribution and to discover novel genes related to the parasitic life history. The A. haemophila ESTs were grouped into 843 clusters and singletons with 658 EST clusters having identifiable homologs, while 159 ESTs were unique and had no similarity to any sequences in the public databases. Not unexpectedly, about 67% of the A. haemophila ESTs have similarity to annotated and hypothetical genes from the related oligohymenophorean ciliate, Tetrahymena. Numerous cysteine proteases, hypothetical proteins and novel sequences possess putative secretory signal peptides suggesting that they may contribute to the pathogenesis of bumper car disease in lobster. Real time RT-qPCR analysis of cathepsin L and two homologs of cathepsin B did not show any changes in gene expression under varying in vitro growth conditions or during a modified-in vivo infection which may be suggestive of the opportunistic life history strategy of this ciliate. Copyright © 2011 Elsevier Inc. All rights reserved.

  19. ESTUDIO EMPÍRICO SOBRE LAS ESTRATEGIAS DE LOGÍSTICA INVERSA EN EL SECTOR INDUSTRIAL DE LA PROVINCIA DE VILLA CLARA

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    Roberto Cespón Castro

    2009-09-01

    Full Text Available

    El presente artículo contiene los principales resultados obtenidos de un estudio empírico sobre logística inversa en el sector industrial de la provincia de Villa Clara. En el mismo se parte de la hipótesis de que es posible identificar una taxonomía de estrategias de logística inversa que se subdivide en tres grandes tipos: Comercial, de Recuperación y Medio Ambiental, las cuales son validadas mediante el estudio. Ello permite identificar el estado del Arte de este importante componente de la Logística de los Negocios en todo un universo de empresas industriales de la mencionada provincia, como vía para una futura secuencia de acciones de mejoramiento.

  20. Effects of pregnancy and delivery on serum concentrations of Clara Cell Protein (CC16), an endogenous anticytokine: lower serum CC16 is related to postpartum depression

    Science.gov (United States)

    Maes; Ombelet; Libbrecht; Stevens; Kenis; De Jongh R; Lin; Cox; Bosmans

    1999-10-11

    There is now some evidence that lower serum concentrations of Clara Cell Protein (CC16) are related to stress-induced anxiety, psychoses and major depression. This study was developed to determine whether serum CC16 is lowered in the early puerperium and whether Postnatal Depression and Postnatal Blues are associated with lower levels of serum CC16. Serum concentrations of CC16 were assayed in 17 non-pregnant women and in 98 pregnant women before delivery and 1 and 3 days after delivery. On each occasion the parturients completed the State version of the Spielberger State-Trait Anxiety Inventory (STAI) and the Zung Depression Rating Scale (ZDS). Serum CC16 was significantly lower in pregnant women, at the end of pregnancy as well as 1 and 3 days after delivery, than in the non-pregnant women. Serum CC16 was somewhat, although significantly, higher 1 and 3 days after delivery than before delivery. Parturients who developed a postpartum depression had significantly lower serum CC16 concentrations than women who did not. There were no significant differences in serum CC16 between the puerperal women whose STAI or ZDS scores increased in the puerperium and those whose scores did not. It is concluded that in puerperal women there is a decreased anti-inflammatory capacity in the serum, in part caused by lowered serum CC16, and that the latter may be related to the development of postpartum depression.

  1. Reporte inicial de las investigaciones arqueológicas en el tercer claustro del antiguo convento de Santa Clara de Asís

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    Darwin A. Arduengo García

    2010-07-01

    Full Text Available Este trabajo propone una nueva lectura del tercer claustro del edificio del antiguo convento de Santa Clara de Asís de la Habana Vieja, sede del Centro Nacional de Conservación, Restauración y Museología (CENCREM. Los antecedentes de los que se parte críticamente son la importante investigación publicada por el historiador Pedro Herrera (2006, así como los reportes de las investigaciones arqueológicas llevadas a cabo en las áreas del edificio durante unos cincuenta años. Durante los últimos cinco años las investigaciones fundamentales realizadas por el grupo de arqueología del CENCREM en lo tocante al proyecto investigativo general del convento han estado dedicadas a las instalaciones hidráulicas del primer claustro y a los cambios constructivos del tercer claustro. Como consecuencia de las investigaciones en el tercer claustro se ha identificado una capilla no reportada en la bibliografía o en fuentes documentales

  2. Gestão da Qualidade de Vida dos idosos frequentadores do Centro de Convivência e Apoio ao idoso Casa de Clara

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    Josimeire Querollaine Alves de Sousa

    2015-06-01

    Full Text Available A longevidade populacional é uma realidade cada vez mais presente na sociedade, em que o número de idosos irá se sobressair ao número de jovens e crianças até 2050, à vista disso, aumenta-se a preocupação em promover a qualidade de vida na terceira idade, no qual o governo por sua vez, criou um programa que incentivasse a prestação de serviços que contribuíssem para o bem-estar dos idosos. O conceito de qualidade de vida e bem-estar, segundo diversos autores, abrange inúmeros aspectos da vida, como o nível sociocultural e econômico, familiar, psicológico e biológico. Este artigo utilizou um estudo de caso no Centro de Convivência e Apoio ao Idoso Casa de Clara, em que foi aplicado o questionário do Ferrans and Powers Quality of Life Index (QLI, desenvolvido mediante rigorosos critérios metodológicos e fundamentado em diversos estudos internacionais.

  3. Análisis de la seguridad y salud laboral en los aprovechamientos forestales de cortas de claras en España

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    Y. Ambrosio Torrijos

    2001-01-01

    Full Text Available A lo largo de ocho años se han controlado de forma directa un total de 34 explotaciones forestales españolas, correspondientes a cortas de claras de masas de repoblación. Se ha reunido información sobre equipos materiales y humanos, riesgos de accidente y para la salud, pautas de seguridad e incidencias ocurridas a lo largo de más de 2,200 horas de cronometraje de las distintas operaciones de explotación y alrededor de 3,000 horas de presencia en el monte. El estudio pone en evidencia las deficiencias de equipamiento activo y pasivo que tienen los equipos utilizados, poniendo especial relevancia en los trabajos realizados manualmente, en donde la falta de profesionalidad, la forma de retribución y la falta de planes reales de seguridad y salud laboral en los aprovechamientos forestales llevan consigo una alta accidentabilidad. Respecto de los riesgos de salud, se ha incorporado como factores de riesgo importantes en los trabajos manuales, la enfermedad de los dedos blancos, causadas por las vibraciones que transmite la motosierra y la enfermedad de Leyme causada por las garrapatas, ambas consideradas enfermedades profesionales en muchos países de Europa pero que en España no se le reconoce su importancia.

  4. Listeria monocytogenes virulence factor Listeriolysin O favors bacterial growth in co-culture with the ciliate Tetrahymena pyriformis, causes protozoan encystment and promotes bacterial survival inside cysts

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    Ermolaeva Svetlana A

    2010-01-01

    Full Text Available Abstract Background The gram-positive pathogenic bacterium Listeria monocytogenes is widely spread in the nature. L. monocytogenes was reported to be isolated from soil, water, sewage and sludge. Listeriolysin O (LLO is a L. monocytogenes major virulence factor. In the course of infection in mammals, LLO is required for intracellular survival and apoptosis induction in lymphocytes. In this study, we explored the potential of LLO to promote interactions between L. monocytogenes and the ubiquitous inhabitant of natural ecosystems bacteriovorous free-living ciliate Tetrahymena pyriformis. Results Wild type L. monocytogenes reduced T. pyriformis trophozoite counts and stimulated encystment. The effects were observed starting from 48 h of co-incubation. On the day 14, trophozoites were eliminated from the co-culture while about 5 × 104 cells/ml remained in the axenic T. pyriformis culture. The deficient in the LLO-encoding hly gene L. monocytogenes strain failed to cause mortality among protozoa and to trigger protozoan encystment. Replenishment of the hly gene in the mutant strain restored toxicity towards protozoa and induction of protozoan encystment. The saprophytic non-haemolytic species L. innocua transformed with the LLO-expressing plasmid caused extensive mortality and encystment in ciliates. During the first week of co-incubation, LLO-producing L. monocytogenes demonstrated higher growth rates in association with T. pyriformis than the LLO-deficient isogenic strain. At latter stages of co-incubation bacterial counts were similar for both strains. T. pyriformis cysts infected with wild type L. monocytogenes caused listerial infection in guinea pigs upon ocular and oral inoculation. The infection was proved by bacterial plating from the internal organs. Conclusions The L. monocytogenes virulence factor LLO promotes bacterial survival and growth in the presence of bacteriovorous ciliate T. pyriformis. LLO is responsible for L. monocytogenes

  5. EVIDENCIA DE ESCORIAS DE COBRE PREHISPÁNICAS EN EL ÁREA DE SANTA CLARA DEL COBRE, MICHOACÁN, OCCIDENTE DE MÉXICO (Evidence of Prehispanic Copper Slags from the Santa Clara del Cobre Area, Michoacan, Western Mexico

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    José Luis Punzo Díaz

    2015-10-01

    Full Text Available En este artículo se presentan los primeros resultados de dataciones para objetos arqueológicos metálicos en Mesoamérica. A través del análisis arqueomagnético de las escorias, producto de la fundición del cobre en siete sitios arqueológicos de los alrededores de Santa Clara del Cobre, Michoacán, se logró ubicarlas cronológicamente; pudiendo determinar la presencia de esta tecnología de fundición desde los últimos cien años antes de la llegada de los españoles, durante el esplendor del Señorío Tarasco, hasta adentrado el siglo XVIII, cuando nuevas tecnologías y una producción masiva de cobre se desarrollaron en todos los territorios bajo el dominio español. ENGLISH: This paper presents the first magnetic dating of metal items in Mesoamerica. The archaeomagnetic analysis of the slags, the residual product of the copper smelting process from seven archaeological sites near the town of Santa Clara del Cobre, Michoacán State, made it possible to estimate their production time. The results obtained thus far suggest the presence of smelting technology in this area 100 years before the Spanish conquest, synchronous of apogee of the Tarascan Señorio, until the XVIII century when the development of new technologies and a massive production of copper occurred in whole territories under the Spanish dominance.

  6. Reconfiguración de la red de 33 kV para pérdidas mínimas con generación distribuida en Villa Clara; 33 kV Network Reconfiguration for Minimum Losses with Distributed Generation in Villa Clara

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    Maylin Medel González

    2011-02-01

    Full Text Available Reconfiguración de circuitos, automatización de la distribución y generación distribuida han sido integrados en elanálisis de la red de 33 kV de la provincia de Villa Clara con el objetivo de encontrar las opciones más adecuadaspara operar dicha red, y su alimentación compuesta por las líneas de 110 kV. Se han encontrado las variantes deoperación con pérdidas mínimas mediante la aplicación del método de reconfiguración de Civanlar utilizando elsoftware PSX como herramienta de flujo de carga para analizar la red de subtransmisión de la provincia. Elanálisis realizado ha permitido encontrar las variantes de operación con pérdidas mínimas de la red de 33 kV, laslíneas de 110 kV y la propuesta de la primera etapa de automatización relacionada con la manipulación adistancia de los desconectivos que conformen las configuraciones óptimas de las redes de 33 kV que tienenemplazamientos sincronizados. The 33 kV feeders reconfiguration taking into consideration the distributed generation and the distant linkbreakers manipulations have been integrated in the Villa Clara power system analysis in order to get the bestoptions to operate it, and the their 110 kV lines.The minimum looses variants have been found applying theCivanlar reconfiguration method using PSX software for load flow studies. Distant manipulations of breakers arestudied in the 33 kV network to achieve the outcomes as a first automation step of the local power system. 

  7. A case study for effects of operational taxonomic units from intracellular endoparasites and ciliates on the eukaryotic phylogeny: phylogenetic position of the haptophyta in analyses of multiple slowly evolving genes.

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    Hisayoshi Nozaki

    Full Text Available Recent multigene phylogenetic analyses have contributed much to our understanding of eukaryotic phylogeny. However, the phylogenetic positions of various lineages within the eukaryotes have remained unresolved or in conflict between different phylogenetic studies. These phylogenetic ambiguities might have resulted from mixtures or integration from various factors including limited taxon sampling, missing data in the alignment, saturations of rapidly evolving genes, mixed analyses of short- and long-branched operational taxonomic units (OTUs, intracellular endoparasite and ciliate OTUs with unusual substitution etc. In order to evaluate the effects from intracellular endoparasite and ciliate OTUs co-analyzed on the eukaryotic phylogeny and simplify the results, we here used two different sets of data matrices of multiple slowly evolving genes with small amounts of missing data and examined the phylogenetic position of the secondary photosynthetic chromalveolates Haptophyta, one of the most abundant groups of oceanic phytoplankton and significant primary producers. In both sets, a robust sister relationship between Haptophyta and SAR (stramenopiles, alveolates, rhizarians, or SA [stramenopiles and alveolates] was resolved when intracellular endoparasite/ciliate OTUs were excluded, but not in their presence. Based on comparisons of character optimizations on a fixed tree (with a clade composed of haptophytes and SAR or SA, disruption of the monophyly between haptophytes and SAR (or SA in the presence of intracellular endoparasite/ciliate OTUs can be considered to be a result of multiple evolutionary reversals of character positions that supported the synapomorphy of the haptophyte and SAR (or SA clade in the absence of intracellular endoparasite/ciliate OTUs.

  8. Effect of high levels of the rotifer Lecane inermis on the ciliate community in laboratory-scale sequencing batch bioreactors (SBRs).

    Science.gov (United States)

    Fyda, Janusz; Babko, Roman; Fiałkowska, Edyta; Pajdak-Stós, Agnieszka; Kocerba-Soroka, Wioleta; Sobczyk, Mateusz; Sobczyk, Łukasz

    2015-10-01

    Due to its ability to feed on filamentous bacteria, the rotifer Lecane inermis has already been recognized as a potential control agent of activated sludge bulking, which is usually caused by the excessive growth of filamentous microorganisms. However, their effectiveness depends, in part, on their abundance. We studied the influence of high densities of L. inermis on the protozoan community in activated sludge from a wastewater treatment plant (WWTP) in 4 laboratory-scale sequencing batch bioreactors (SBRs). Two treatments and two controls were subjected to nutrient removal system in process similar to that used in a WWTP. The experiment lasted 9 days and was repeated in 24-h cycles, including phases of agitation with feeding, aeration and agitation and sedimentation with decantation at the end of the cycle. In total, 32 taxa were identified, among which 25 were ciliated protozoa, 4 were amoebae, 2 were flagellates, and one was a nematode. Rotifers were then introduced to 2 bioreactors at a final concentration of 500ind.mL(-1), and the taxonomic composition and abundance of the activated sludge microfauna were assessed 2, 5 and 8 days thereafter. The mean density of ciliates on the first day of experiment was 12,610ind.mL(-1) and diminished to 4868±432ind.mL-±432ind.mL(-1) in the control and 5496±638ind.mL(-1) in the rotifer-treated group on the last day. Thus, even extremely high densities of artificially introduced rotifers did not negatively affect the protozoan community. On the contrary, the protozoan community was more diverse in the treatment group than in the control. Copyright © 2015 Elsevier GmbH. All rights reserved.

  9. The influence of gestation and mechanical ventilation on serum clara cell secretory protein (CC10) concentrations in ventilated and nonventilated newborn infants.

    Science.gov (United States)

    Loughran-Fowlds, Alison; Oei, Julee; Wang, He; Xu, Hongxiu; Wimalasundera, Neil; Egan, Claire; Henry, Richard; Lui, Kei

    2006-07-01

    Clara cell secretory protein (CC10) is an important anti-inflammatory mediator in the adult lung, but its role in newborn pulmonary protection is uncertain. We examined the early postnatal behavior of CC10 in newborn serum and tracheal fluid and hypothesized that CC10 production is positively influenced by gestation. Blood from 165 infants from the first, third/fourth, and seventh days of life (gestational ages: 23-29 wk, 30-36 wk, >36 wk) and tracheal fluid (TF) from the first day of life from 32 ventilated infants were analyzed for CC10. Surfactant proteins A (SPA) and B (SPB) were also analyzed from the blood of a subgroup of infants. Serum CC10 on day 1 was highest in term infants (69.4 ng/mL), followed by moderately preterm (55.8 ng/mL), and then extremely preterm infants (median 42.1 ng/mL). Term infants also had higher tracheal fluid CC10 than preterm infants. (20.152 ng/mL versus 882 ng/mL). Mechanical ventilation increased serum CC10 only in moderately preterm infants, and only on d 1 [68.4 ng/mL versus 42.1 ng/mL (nonventilated moderately preterm infants)]. Serum CC10 decreased progressively by the end of the first week in all infants, in contrast to SPA and SPB, which increased. Our results show that CC10 is detectable in the blood of newborn infants and that a production surge occurs at birth. This surge is more pronounced in term infants and may confer them with superior extrauterine pulmonary protection compared with preterm infants.

  10. ¿Favoritas de la corona? Los amores del rey y la promoción de la orden de Santa Clara en Castilla (ss. XIII-XIV

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    Graña Cid, María del Mar

    2014-06-01

    Full Text Available The promotion of female mendicant houses by the kings of Castile offers a typology of “partnering foundations” associated with women. Alfonso X and Pedro I collaborated with their lovers, Mayor Guillén and María de Padilla, and the latter also with his daughters, Beatriz and Isabel, in order to create monasteries of the Order of Saint Clare or its previous forms. These pages analyse the foundational processes that connect the familiar with the political and the personal, offer visible forms of interaction between sexes and reveal some of the physiognomies adopted by the nuns that constituted an exclusively female religious order in which women acted as its main historical agents.En la política de promoción de las monjas mendicantes que desarrollaron los reyes de Castilla se percibe una tipología de “fundaciones en relación” con mujeres. Alfonso X y Pedro I colaboraron con sus amantes, Mayor Guillén y María de Padilla, y el segundo también con sus hijas, Beatriz e Isabel, para dar origen a monasterios de la Orden de Santa Clara o de sus formas previas. Estas páginas analizan unos procesos de fundación que enlazan lo personal y lo familiar con lo político, visibilizan formas de interacción entre los sexos y desvelan algunas de las fisonomías adoptadas por las religiosas que constituyeron la institución clarisa, una orden religiosa exclusivamente femenina en la que tuvieron gran peso las mujeres como agentes históricos.

  11. Short baseline variations in site response and wave-propagation effects and their structural causes: Four examples in and around the santa clara valley, California

    Science.gov (United States)

    Hartzell, S.; Ramirez-Guzman, L.; Carver, D.; Liu, P.

    2010-01-01

    Ground motion records of local and regional events from a portable array are used to investigate the structural causes of variations in ground motion over distances of a few hundred meters to a few kilometers in the sedimentary basin environment of the Santa Clara Valley, California, and its margins. Arrays of portable seismic stations are used to target four study areas with different ground motion patterns: (1) an edge of the alluvial basin extending up onto a marginal ridge (Blossom Hill), (2) a Cenozoic basin with a nearly flat bottom (Cupertino Basin), (3) a long, narrow Cenozoic basin with a steep V profile (Evergreen Basin), and (4) a line perpendicular to the trace of the Hayward fault. Average peak velocities on Blossom Hill from local earthquakes are a factor of 2.5 times higher than nearby valley sites. Three-dimensional (3D) modeling is used to conclude that the majority of the amplification is due to lower shear-wave velocities along a local fault zone (Shannon–Berrocal). Site amplification over the Cupertino Basin in the frequency band 0.5–4 Hz is generally low (less than 2.0 relative to a Mesozoic rock site) and spatially uniform. This response is attributed to the shallow, flat-bottomed shape of the basin and the uniform, flat-laying sedimentary fill. In contrast, site amplification in the Evergreen Basin generally exceeds 3.0 and is attributed to the deep, V-shaped geometry of the basin and younger sedimentary fill. 3D waveform modeling shows the elongated shape of the Evergreen Basin causes more efficient trapping of long-period waves for sources along the long axis of the basin. A low-velocity zone is postulated along the Hayward fault with a width between 100 and 200 m, based on elevated site response along the fault trace and 4.5-Hz fault zone guided waves on the horizontal components of stations near the fault.

  12. Remote Sensing of Ground Deformation for Monitoring Groundwater Management Practices: Application to the Santa Clara Valley During the 2012-2015 California Drought

    Science.gov (United States)

    Chaussard, Estelle; Milillo, Pietro; Bürgmann, Roland; Perissin, Daniele; Fielding, Eric J.; Baker, Brett

    2017-10-01

    Groundwater management typically relies on water-level data and spatially limited deformation measurements. While interferometric synthetic aperture radar has been used to study hydrological deformation, its limited temporal sampling can lead to biases in rapidly changing systems. Here we use 2011-2017 COSMO-SkyMed data with revisit intervals as short as 1 day to study the response of the Santa Clara Valley (SCV) aquifer in California to the unprecedented 2012-2015 drought. Cross-correlation and independent component analyses of deformation time series enable tracking water through the aquifer system. The aquifer properties are derived prior to and during the drought to assess the success of water-resource management practices. Subsidence due to groundwater withdrawal dominates during 2011-2017, limited to the confined aquifer and west of the Silver Creek Fault, similar to predrought summer periods. Minimum water levels and elevations were reached in mid-2014, but thanks to intensive groundwater management efforts the basin started to rebound in late 2014, during the deepening drought. By 2017, water levels were back to their predrought levels, while elevations had not yet fully rebounded due to the delayed poroelastic response of aquitards and their large elastic compressibility. As water levels did not reach a new lowstand, the drought led to only elastic and recoverable changes in the SCV. The SCV lost 0.09 km3 during the drought while seasonal variations amount to 0.02 km3. Analysis of surface loads due to water mass changes in the aquifer system suggests that groundwater drawdowns could influence the stress on nearby faults.

  13. CARCINOMA ODONTOGÉNICO DE CELULAS CLARAS. ESTUDIO CLÍNICO, RADIOLÓGICO Y PATOLÓGICO DE UN CASO.

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    Rosaura Rodríguez Flores

    2004-01-01

    Full Text Available Los tumores odontogénicos (TO son un grupo complejo y poco frecuente de neoplasias con características clínicas, radiológicas, histopatológicas y evolución muy variables. El Carcinoma odontogénico de células claras (COCC es una neoplasia maligna, de comportamiento agresivo variable que se presenta en maxilares. Histológicamente se constituye de nidos de células, frecuentemente de citoplasma claro, rico en glucógeno, rodeadas por bandas de colágena. Pertenece al grupo de los tumores odontogénicos epiteliales malignos. Se presenta el caso de un hombre de 57 años con un tumor en hueso y seno maxilar izquierdo, sometido a hemimaxilectomía subtotal izquierda. Los estudios de imagen demostraron una lesión destructiva mixta en maxilar y seno maxilar izquierdo y la histología reveló la imagen clásica de COCC y la inmunohistoquímica mostró inmunoreactividad para Queratina AE1/AE3, EMA y S-100 (focal. Se demostró abudante glucógeno mediante la reacción de PAS en el citoplasma de las células neoplásicas. El paciente ha tenido seguimiento por 5 años y está asintomático después de la cirugía sin tratamiento complementario con radioterapia.

  14. Geologic, water-chemistry, and hydrologic data from multiple-well monitoring sites and selected water-supply wells in the Santa Clara Valley, California, 1999-2003

    Science.gov (United States)

    Newhouse, M.W.; Hanson, R.T.; Wentworth, C.M.; Everett, Rhett; Williams, C.F.; Tinsley, J.C.; Noce, T.E.; Carkin, B.A.

    2004-01-01

    To better identify the three-dimensional geohydrologic framework of the Santa Clara Valley, lithologic, geologic, geophysical, geomechanical, hydraulic, and water-chemistry data were collected from eight ground-water multiple-well monitoring sites constructed in Santa Clara County, California, as part of a series of cooperative studies between the U.S. Geological Survey and the Santa Clara Valley Water District. The data are being used to update and improve the three-dimensional geohydrologic framework of the basin and to address issues related to water supply, water chemistry, sequence stratigraphy, geology, and geological hazards. This report represents a compilation of data collected from 1999 to 2003, including location and design of the monitoring sites, cone penetrometer borings, geologic logs, lithologic logs, geophysical logs, core analysis, water-chemistry analysis, ground-water-level measurements, and hydraulic and geomechanical properties from wells and core samples. Exploratory cone penetrometer borings taken in the upper 17 to 130 feet at six of the monitoring sites identified the base of Holocene as no deeper than 75 feet in the central confined area and no deeper than 35 feet in the southern unconfined areas of the valley. Generalized lithologic characterization from the monitoring sites indicates about four to six different aquifer units separated by relatively fine-grained units occur within the alluvial deposits shallower than 860 feet deep. Analysis of geophysical logs indicates that coarse-grained units varied in thickness between 10 and 25 feet in the southeastern unconfined area of the valley and between 50 and 200 feet in the south-central and southwestern areas of the valley. Deviations from temperature-gradient logs indicate that the majority of horizontal ground-water flow occurs above a depth of 775 feet in the south central and above 510 feet in the southeastern areas of the valley. Bulk physical properties from more than 1,150 feet of

  15. Impacto de un programa de detección de conductores bajo los efectos del alcohol en la prevención de accidentes de tráfico: provincia de Villa Clara [Cuba] Impact of a drink-driving detection program to prevent traffic accidents: Villa Clara Province, Cuba

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    Humberto Guanche Garcell

    2008-08-01

    Full Text Available Objetivo: Determinar la efectividad de una metodología dirigida a la reducción de los accidentes de tráfico y sus consecuencias en la provincia de Villa Clara (Cuba. Diseño: Durante el período de mayo-octubre del año 2003, se realizó vigilancia activa sobre la ingestión de bebidas alcohólicas por parte de los conductores. Se utilizaron técnicas de análisis de series cronológicas. Resultados: Se detectaron 805 (12,46% conductores bajo los efectos del alcohol. Se constató un descenso de los accidentes (29,9%, las defunciones (70,8% y los lesionados (58,7% en comparación con un período similar del año 2002. La reducción de los accidentes fue inferior al límite histórico en mayo, junio, julio, septiembre y octubre, mientras que las defunciones fueron menores en mayo, agosto y septiembre. Los lesionados disminuyeron por debajo del límite histórico en el mes de julio. Conclusión: La intervención realizada es una medida útil para la prevención de accidentes de tráfico y sus consecuencias.Objective: To determine the effectiveness of a method to reduce road traffic accidents and their consequences in the province of Villa Clara (Cuba. Design: From May to October 2003, active surveillance of drink-driving was carried out. Analysis of chronological series was used. Results: A total of 805 (12.46% drivers were found to be under the effect of alcohol. Traffic accidents, deaths, and injuries decreased by 29.9%, 70.8% and 58.7%, respectively, in comparison with a similar period in 2002. The number of accidents in May, June, July, September and October fell below the historical low. The number of deaths was lowest in May, August and September. The number of injuries in July fell below the historical low. Conclusion: The interventions carried out proved useful in preventing traffic accidents and their consequences.

  16. HYDRAULICS, SANTA CLARA COUNTY, CA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  17. Evaluación y modificación del Sistema de Tratamiento de Aguas Residuales de la Planta de Matanza del ITCR en Santa Clara, San Carlos

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    Alma Deloya Martínez

    2006-05-01

    Full Text Available La planta de matanza de ganado vacuno y porcino del ITCR, Sede San Carlos, en Santa Clara, procesa en promedio 23 reses y 11 cerdos por día. El caudal de aguas residuales promedio generado durante el proceso de matanza varió de un máximo de 72 m3/día hasta un mínimo de 12 m3/día. Las aguas residuales son tratadas en un sistema de tratamiento biológico anaerobio, para controlar la contaminación del agua, según el Reglamento de vertido y reúso de aguas residuales. El sistema original consistió en un tanque de aireación y un sedimentador integrado al tanque de aireación. La aireación se suministraba por rotores mecánicos. Durante el desarrollo del proyecto, se encontró que el principal problema en la operación y control del sistema eran los sólidos suspendidos totales y los sólidos sedimentables, no presentándose problemas de calidad del efluente en términos de DBO, DQO y otros parámetros. Para solucionar el problema de los sólidos, en el efluente del sistema de tratamiento se modificó el sistema aerobio existente a un sistema anaerobio (para reducir la producción de sólidos, usando como ayuda del tratamiento un compuesto biológico, que actúa como floculante y coagulante, lo que mejora la sedimentación de los sólidos. Con la modificación del sistema, se alcanzaron eficiencias de remoción de los sólidos sedimentables en un 95%, de la demanda bioquímica de oxígeno DBO en un 83% y la DQO en un 75%, cumpliéndose con la normativa para todos los parámetros exigidos por el Ministerio de Salud.

  18. Ciliate and mesozooplankton community response to increasing CO2 levels in the Baltic Sea: insights from a large-scale mesocosm experiment

    Science.gov (United States)

    Lischka, Silke; Bach, Lennart T.; Schulz, Kai-Georg; Riebesell, Ulf

    2017-01-01

    Community approaches to investigating ocean acidification (OA) effects suggest a high tolerance of micro- and mesozooplankton to carbonate chemistry changes expected to occur within this century. Plankton communities in the coastal areas of the Baltic Sea frequently experience pH variations partly exceeding projections for the near future both on a diurnal and seasonal basis. We conducted a large-scale mesocosm CO2 enrichment experiment ( ˜ 55 m3) enclosing the natural plankton community in Tvärminne-Storfjärden for 8 weeks during June-August 2012 and studied community and species-taxon response of ciliates and mesozooplankton to CO2 elevations expected for this century. In addition to the response to fCO2, we also considered temperature and chlorophyll a variations in our analyses. Shannon diversity of ciliates significantly decreased with fCO2 and temperature with a greater dominance of smaller species. The mixotrophic Myrionecta rubra seemed to indirectly and directly benefit from higher CO2 concentrations in the post-bloom phase through increased occurrence of picoeukaryotes (most likely Cryptophytes) and Dinophyta at higher CO2 levels. With respect to mesozooplankton, we did not detect significant effects for either total abundance or for Shannon diversity. The cladocera Bosmina sp. occurred at distinctly higher abundance for a short time period during the second half of the experiment in three of the CO2-enriched mesocosms except for the highest CO2 level. The ratio of Bosmina sp. with empty to embryo- or resting-egg-bearing brood chambers, however, was significantly affected by CO2, temperature, and chlorophyll a. An indirect CO2 effect via increased food availability (Cyanobacteria) stimulating Bosmina sp. reproduction cannot be ruled out. Although increased regenerated primary production diminishes trophic transfer in general, the presence of organisms able to graze on bacteria such as cladocerans may positively impact organic matter transfer to higher

  19. Relato de uma experiência utilizando-se a clara de ovo na dermatite amoniacal no centro de convivência infantil do Instituto "Dante Pazzanese" de cardiologia

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    Lindalva de Jesus da Silva

    1991-09-01

    Full Text Available As autoras deste trabalho se propõem mostrar a atuação de clara de ovo nas dermatites amoniacais, em crianças que freqüentam o Centro de Convivência Infantil do Instituto "Dante Pazzanese" de Cardiologia, avaliando a eficácia de sua utilização. Para isso, apresentam 35 casos de assadura ocorridos no período de janeiro a junho de 1988, com resultados satisfatórios.

  20. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples

    Science.gov (United States)

    Su, Lei; Zhang, Qianqian; Gong, Jun

    2017-07-01

    Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifications of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study, two peritrich-specific PCR primers were newly designed to amplify a fragment including the internal transcribed spacer (ITS) region of ribosomal rDNA from environmental samples. The primers showed high specificity in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18S rDNA in peritrichs. The newly designed specific primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in different systems.

  1. Timing of perialgal vacuole membrane differentiation from digestive vacuole membrane in infection of symbiotic algae Chlorella vulgaris of the ciliate Paramecium bursaria.

    Science.gov (United States)

    Kodama, Yuuki; Fujishima, Masahiro

    2009-02-01

    Each symbiotic Chlorella of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole to protect from lysosomal fusion. To understand the timing of differentiation of the perialgal vacuole from the host digestive vacuole, algae-free P. bursaria cells were fed symbiotic C. vulgaris cells for 1.5min, washed, chased and fixed at various times after mixing. Acid phosphatase activity in the vacuoles enclosing the algae was detected by Gomori's staining. This activity appeared in 3-min-old vacuoles, and all algae-containing vacuoles demonstrated activity at 30min. Algal escape from these digestive vacuoles began at 30min by budding of the digestive vacuole membrane into the cytoplasm. In the budded membrane, each alga was surrounded by a Gomori's thin positive staining layer. The vacuoles containing a single algal cell moved quickly to and attached just beneath the host cell surface. Such vacuoles were Gomori's staining negative, indicating that the perialgal vacuole membrane differentiates soon after the algal escape from the host digestive vacuole. This is the first report demonstrating the timing of differentiation of the perialgal vacuole membrane during infection of P. bursaria with symbiotic Chlorella.

  2. Historical trends in the species inventory of tintinnids (ciliates of the microzooplankton) in the Bay of Villefranche (NW Mediterranean Sea): Shifting baselines.

    Science.gov (United States)

    Dolan, John R

    2017-02-01

    In the Bay of Villefranche, tintinnid ciliates have been studied since 1879 yielding a unique time series. The species inventory, excluding likely synonymous records, numbers 108. Temporal increases in the inventory appear linearly related to sampling effort up until the 2000s with a cumulative sampling effort of about 200 dates. Subsequently, with a large increase in sampling to currently over 460 dates, the rate of increases in species numbers declines. Surprisingly, the inventory is not highly inflated by unique occurrences, as species found but once are only 17 out of the 108. However, in recent years many previously recorded taxa have not been seen. Missing from a species list derived solely from intensive sampling from 2013 to 2016 are 38 previously recorded species. Most (26 out of 38) were recorded from a single year and thus may have been temporary residents. However, 12 species were found in multiple years by different investigators suggesting relatively common occurrence in the past. The substantial effort required to adequately sample a locality and possible historical changes in species inventories suggest that claims of a species as 'new' to a locality should be made with caution & caveats. Copyright © 2016 Elsevier GmbH. All rights reserved.

  3. Cell division and density of symbiotic Chlorella variabilis of the ciliate Paramecium bursaria is controlled by the host's nutritional conditions during early infection process.

    Science.gov (United States)

    Kodama, Yuuki; Fujishima, Masahiro

    2012-10-01

    The association of ciliate Paramecium bursaria with symbiotic Chlorella sp. is a mutualistic symbiosis. However, both the alga-free paramecia and symbiotic algae can still grow independently and can be reinfected experimentally by mixing them. Effects of the host's nutritional conditions against the symbiotic algal cell division and density were examined during early reinfection. Transmission electron microscopy revealed that algal cell division starts 24 h after mixing with alga-free P. bursaria, and that the algal mother cell wall is discarded from the perialgal vacuole membrane, which encloses symbiotic alga. Labelling of the mother cell wall with Calcofluor White Stain, a cell-wall-specific fluorochrome, was used to show whether alga had divided or not. Pulse labelling of alga-free P. bursaria cells with Calcofluor White Stain-stained algae with or without food bacteria for P. bursaria revealed that the fluorescence of Calcofluor White Stain in P. bursaria with bacteria disappeared within 3 days after mixing, significantly faster than without bacteria. Similar results were obtained both under constant light and dark conditions. This report is the first describing that the cell division and density of symbiotic algae of P. bursaria are controlled by the host's nutritional conditions during early infection. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

  4. Symbiotic Chlorella sp. of the ciliate Paramecium bursaria do not prevent acidification and lysosomal fusion of host digestive vacuoles during infection.

    Science.gov (United States)

    Kodama, Yuuki; Fujishima, Masahiro

    2005-10-01

    Each symbiotic Chlorella sp. of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole, and thereby the alga is protected from digestion by lysosomal fusion. Algae-free cells can be reinfected with algae isolated from algae-bearing cells by ingestion into digestive vacuoles. To examine the timing of acidification and lysosomal fusion of the digestive vacuoles and of algal escape from the digestive vacuole, algae-free cells were mixed with isolated algae or yeast cells stained with pH indicator dyes at 25+/-1 degrees C for 1.5 min, washed, chased, and fixed at various time points. Acidification of the vacuoles and digestion of Chlorella sp. began at 0.5 and 2 min after mixing, respectively. All single green Chlorella sp. that had been present in the host cytoplasm before 0.5 h after mixing were digested by 0.5 h. At 1 h after mixing, however, single green algae reappeared in the host cytoplasm, arising from those digestive vacuoles containing both nondigested and partially digested algae, and the percentage of such cells increased to about 40% at 3 h. At 48 h, the single green algae began to multiply by cell division, indicating that these algae had succeeded in establishing endosymbiosis. In contrast to previously published studies, our data show that an alga can successfully escape from the host's digestive vacuole after acidosomal and lysosomal fusion with the vacuole has occurred, in order to produce endosymbiosis.

  5. An Antarctic hypotrichous ciliate, Parasterkiella thompsoni (Foissner) nov. gen., nov. comb., recorded in Argentinean peat-bogs: morphology, morphogenesis, and molecular phylogeny.

    Science.gov (United States)

    Küppers, Gabriela Cristina; Paiva, Thiago da Silva; Borges, Bárbara do Nascimento; Harada, Maria Lúcia; Garraza, Gabriela González; Mataloni, Gabriela

    2011-05-01

    The ciliate Parasterkiella thompsoni (Foissner, 1996) nov. gen., nov. comb. was originally described from Antarctica. In the present study, we report the morphology, morphogenesis during cell division, and molecular phylogeny inferred from the 18S-rDNA sequence of a population isolated from the Rancho Hambre peat bog, Tierra del Fuego Province (Argentina). The study is based on live and protargol-impregnated specimens. Molecular phylogeny was inferred from trees constructed by means of the maximum parsimony, neighbor joining, and Bayesian analyses. The interphase morphology matches the original description of the species. During the cell division, stomatogenesis begins with the de novo proliferation of two fields of basal bodies, each one left of the postoral ventral cirri and of transverse cirri, which later unify. Primordia IV-VI of the proter develop from disaggregation of cirrus IV/3, while primordium IV of the opisthe develops from cirrus IV/2 and primordia V and VI from cirrus V/4. Dorsal morphogenesis occurs in the Urosomoida pattern-that is, the fragmentation of kinety 3 is lacking. Three macronuclear nodules are generated before cytokinesis. Phylogenetic analyses consistently placed P. thompsoni within the stylonychines. New data on the morphogenesis of the dorsal ciliature justifies the transference of Sterkiella thompsoni to a new genus Parasterkiella. Copyright © 2011 Elsevier GmbH. All rights reserved.

  6. Morphology and SSU rDNA sequence analysis of two hypotrichous ciliates (Protozoa, Ciliophora, Hypotrichia) including the new species Metaurostylopsis parastruederkypkeae n. sp.

    Science.gov (United States)

    Lu, Borong; Wang, Chundi; Huang, Jie; Shi, Yuhong; Chen, Xiangrui

    2016-10-01

    The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana (Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit (SSU) ribosomal RNA gene (rRNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about (165-200) × (45-60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26-41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU rDNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.

  7. Análisis de las fuentes de información sobre medicamentos de carácter personal en la red de farmacias del municipio Santa Clara Analysis of personal sources of information on drugs present in the network of pharmacies of Santa Clara municipality

    Directory of Open Access Journals (Sweden)

    Manuel Osvaldo Machado Rivero

    2008-12-01

    Full Text Available Se realizó un análisis de las fuentes de información sobre medicamentos no documentales (personales, disponibles en la red de farmacias del municipio Santa Clara. Este estudio tuvo como objetivo la evaluación de las fuentes de información no documentales de carácter personal con que cuenta dicha red. Se incluyeron en esta investigación 26 unidades de farmacias comunitarias. Se diseñó un modelo de recogida de datos para recopilar información sobre el tipo de fuente sujeto de análisis en el presente trabajo. Se encuestaron 7 licenciados en Ciencias Farmacéuticas, profesionales responsables del servicio de información en las distintas unidades de la red de farmacias comunitarias. Paralelamente se estudió la posible función que podían tener los técnicos de Farmacia en dicho servicio, para lo cual se encuestaron a 47 personas que se desempeñan en esa profesión. Los resultados permitieron determinar que existen dificultades con la accesibilidad de la población a estas fuentes; los profesionales de nivel superior no ejercen de manera diaria su función como fuentes de información; se debe profundizar en la superación de técnicos y licenciados, así como en la participación en eventos y la publicación de artículos. No existieron diferencias significativas entre la cantidad de encuestados que refieren emplear la forma personal, responder a consultas telefónicas, o aquellos que combinan ambas formas cotidianamente para brindar información sobre medicamentos.The objective of this study was the evaluation of non-documentary (personal sources of information available in the pharmacy network of Santa Clara municipality. To this end, twenty six community-based pharmacies were included. A data collection model to gather information on the type of source under analysis in this paper was designed. Seven Bachelors of Pharmaceutical Sciences, who are the professionals in charge of the information service in the network of community

  8. Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila: A case of functional divergence in a multigene family

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    Yuan Dongxia

    2010-10-01

    Full Text Available Abstract Background In eukaryotes, ABC transporters that utilize the energy of ATP hydrolysis to expel cellular substrates into the environment are responsible for most of the efflux from cells. Many members of the superfamily of ABC transporters have been linked with resistance to multiple drugs or toxins. Owing to their medical and toxicological importance, members of the ABC superfamily have been studied in several model organisms and warrant examination in newly sequenced genomes. Results A total of 165 ABC transporter genes, constituting a highly expanded superfamily relative to its size in other eukaryotes, were identified in the macronuclear genome of the ciliate Tetrahymena thermophila. Based on ortholog comparisons, phylogenetic topologies and intron characterizations, each highly expanded ABC transporter family of T. thermophila was classified into several distinct groups, and hypotheses about their evolutionary relationships are presented. A comprehensive microarray analysis revealed divergent expression patterns among the members of the ABC transporter superfamily during different states of physiology and development. Many of the relatively recently formed duplicate pairs within individual ABC transporter families exhibit significantly different expression patterns. Further analysis showed that multiple mechanisms have led to functional divergence that is responsible for the preservation of duplicated genes. Conclusion Gene duplications have resulted in an extensive expansion of the superfamily of ABC transporters in the Tetrahymena genome, making it the largest example of its kind reported in any organism to date. Multiple independent duplications and subsequent divergence contributed to the formation of different families of ABC transporter genes. Many of the members within a gene family exhibit different expression patterns. The combination of gene duplication followed by both sequence divergence and acquisition of new patterns of

  9. La estria supranuclear de las células ciliadas en la rinitis alérgica Supranuclear stria of ciliated cells in allergic rhinitis

    Directory of Open Access Journals (Sweden)

    Ana Zerdiew

    2007-08-01

    Full Text Available Se estudiaron 80 pacientes adultos alérgicos, que cursaron con los siguientes cuadros clínicos: 16 casos de rinitis intermitente y 64 de rinitis persistente. Se realizó el recuento porcentual de la estría supranuclear de las células ciliadas, respecto de los leucocitos presentes en los extendidos obtenidos por toma endonasal. Con los datos obtenidos se clasificaron los extendidos en 4 grupos; Grupo A (N=23: predominio leucocitario eosinófilo con eosinofilia nasal >10%, Grupo B (N=15: abundantes leucocitos neutrófilos y eosinofilia nasal >10%, Grupo C (N=29: con escasos leucocitos, Grupo D (N=13: con abundantes leucocitos de predominio neutrófilo sin eosinofilia. Se observó que el incremento porcentual de estría supranuclear se correlacionó con eosinofilia nasal >10% y con las muestras que presentaron escasos leucocitos. Sin embargo se evidenció una marcada disminución del porcentaje de estría supranuclear en la leucocitosis neutrófila de etiología bacteriana.Nasal secretions were studied in 80 allergic adults patients: 16 with intermittent rhinitis and 64 with persistent rhinitis. The percentage of supranuclear stria of ciliated cells with regard to leucocytes was studied by nasal scraping. Four groups of patients were classified according to nasal leucocytic predominance: patients with eosinophilic predominance with eosinophils > 10% in Group A (N=23, patients with abundant neutrophils and eosinophils >10% in Group B (N=15, patients with scant leucocytes in Group C (N=29, patients with neutrophilic predominance without eosinophils in Group D (N=13. An increase of supranuclear stria percentage was correlated to eosinophils > 10% and also correlated to scant leucocytes. Nevertheless, a significant decrease of supranuclear stria percentage was observed in neutrophilic leukocytosis of bacterial etiology.

  10. The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: Involvement of interleukin-2 system

    Energy Technology Data Exchange (ETDEWEB)

    Cervia, Davide, E-mail: d.cervia@unitus.it [Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Viterbo (Italy); Department of Biomedical and Clinical Sciences, “Luigi Sacco” University Hospital, University of Milan, Milano (Italy); Catalani, Elisabetta; Belardinelli, Maria Cristina [Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Viterbo (Italy); Perrotta, Cristiana [Department of Biomedical and Clinical Sciences, “Luigi Sacco” University Hospital, University of Milan, Milano (Italy); Picchietti, Simona [Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Viterbo (Italy); Alimenti, Claudio [Department of Environmental and Natural Sciences, University of Camerino, Camerino (Italy); Casini, Giovanni; Fausto, Anna Maria [Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Viterbo (Italy); Vallesi, Adriana [Department of Environmental and Natural Sciences, University of Camerino, Camerino (Italy)

    2013-02-01

    Water-soluble protein signals (pheromones) of the ciliate Euplotes have been supposed to be functional precursors of growth factors and cytokines that regulate cell–cell interaction in multi-cellular eukaryotes. This work provides evidence that native preparations of the Euplotes raikovi pheromone Er-1 (a helical protein of 40 amino acids) specifically increases viability, DNA synthesis, proliferation, and the production of interferon-γ, tumor necrosis factor-α, interleukin (IL)-1β, IL-2, and IL-13 in human Jurkat T-cells. Also, Er-1 significantly decreases the mRNA levels of the β and γ subunits of IL-2 receptor (IL-2R), while the mRNA levels of the α subunit appeared to be not affected. Jurkat T-cell treatments with Er-1 induced the down-regulation of the IL-2Rα subunit by a reversible and time-dependent endocytosis, and increased the levels of phosphorylation of the extracellular signal-regulated kinases (ERK). The cell-type specificity of these effects was supported by the finding that Er-1, although unable to directly influence the growth of human glioma U-373 cells, induced Jurkat cells to synthesize and release factors that, in turn, inhibited the U-373 cell proliferation. Overall, these findings imply that Er-1 coupling to IL-2R and ERK immuno-enhances T-cell activity, and that this effect likely translates to an inhibition of glioma cell growth. -- Highlights: ► Euplotes pheromone Er-1 increases the growth of human Jurkat T-cells. ► Er-1 increases the T-cell production of specific cytokines. ► Er-1 activates interleukin-2 receptor and extracellular signal-regulated kinases. ► The immuno-enhancing effect of Er-1 on Jurkat cells translates to an inhibition of human glioma cell growth.

  11. Avaliação histopatológica e histoquímica das células claras em lesões odontogênicas císticas

    Directory of Open Access Journals (Sweden)

    Cláudia Roberta Leite Vieira de Figueiredo

    Full Text Available Alterações degenerativas e metaplásicas são usualmente observadas no revestimento epitelial dos cistos odontogênicos. Nos tumores odontogênicos estes processos são considerados mais raros. Objetivo: demonstrar a presença de células claras no revestimento epitelial de uma série de lesões odontogênicas císticas. Métodos: realizou-se um estudo descritivo, em uma amostra de 22 lâminas histológicas, do total de lesões odontogênicas císticas procedentes do Laboratório de Patologia Bucal da Universidade Federal da Paraíba. As lâminas foram examinadas por dois avaliadores previamente calibrados e os blocos parafinados correspondentes às lesões em que se observaram células claras, foram novamente processados, e as lâminas foram coradas pela técnica do Ácido Periódico de Schiff após digestão pela diástase e avaliadas quanto à presença de células mucosas. Resultados: células contendo mucina estavam presentes em 12 (54,5 % das lesões coradas pelo Ácido Periódico de Schiff com diástase. Células mucosas foram observadas em 5 (41,66 % dos 12 casos de cistos radiculares, 1 (50 % dos 2 casos de tumores odontogênicos ceratocísticos, nos 4 (100 % casos de cistos dentígeros e em 2 (100 % casos de ameloblastomas unicísticos. Células claras foram muitas vezes observadas em áreas de inflamação. Conclusão: na amostra estudada, células mucosas puderam ser evidenciadas ocasionalmente no epitélio de lesões odontogênicas císticas.

  12. Moss ciliated protozoa as bioindicators. A study on the urban area of Pisa (Italy); Protozoi ciliati del muschio come biondicatori. Uno studio nell'area urbana di Pisa

    Energy Technology Data Exchange (ETDEWEB)

    Verni, F.; Rosati, G. [Pisa Univ., Pisa (Italy). Dipt. di Etologia, Ecologia, Evoluzione

    2000-07-01

    Samples of moss were collected, every month for 7 months, from five zones of Pisa (S2, S3, S4, S5, S6) and a woody zone (S1) as control. For each sample the type and the number of the ciliate species as well as the number of individuals for each species were determined. Lead content was also measured. The data were statistically elaborated and compared. The diversity Shannon index (H) was calculated to determine the health state of ciliate communities. In any case an inverse correlation between H and the lead content was reported; the highest the H value, the lowest the lead content and vice versa. On the basis of the results here reported the use of Ciliates as biondicators appears suitable for the control of urban soil. An analysis like this appears advantageous as it is rather simple and cheap and allows to determine the possible variations in a real time. [Italian] Sono stati analizzati campioni di muschio prelevati, con cadenza mensile per 7 mesi, in 5 stazioni di Pisa (S2, S3, S4, S5, S6) e da una zona boscosa (S1), come controllo. Per ogni campione si e' proceduto all'identificazione dei Ciliati presenti, al conteggio delle specie e del numero di individui per specie, nonche' alla misurazione del piombo presente. I dati sono stati confrontati dopo elaborazione statistica. L'indice della diversita' di Shannon ha fornito informazioni sulla salute della comunita' dei Protozoi delle varie stazioni. In ogni caso risulta una correlazione inversa tra i valori dell'indice di Shannon e la quantita' di piombo: S5, la stazione piu' esposta al traffico, risulta quella piu' degradata. L'uso dei Ciliati come biondicatori risulta quindi appropriato e vantaggioso considerando che e' semplice, poco costoso e permette di valutare in tempi reali variazioni nell'ambiente.

  13. Breaks in Pavement and Pipes as Indicators of Range-Front Faulting Resulting from the 1989 Loma Prieta Earthquake near the Southwest Margin of the Santa Clara Valley, California

    Science.gov (United States)

    Schmidt, Kevin M.; Ellen, Stephen D.; Haugerud, Ralph A.; Peterson, David M.; Phelps, Geoffery A.

    1995-01-01

    Damage to pavement and near-surface utility pipes, caused by the October 17, 1989, Loma Prieta earthquake, provide indicators for ground deformation in a 663 km2 area near the southwest margin of the Santa Clara Valley, California. The spatial distribution of 1284 sites of such damage documents the extent and distribution of detectable ground deformation. Damage was concentrated in four zones, three of which are near previously mapped faults. The zone through Los Gatos showed the highest concentration of damage, as well as evidence for pre- and post-earthquake deformation. Damage along the foot of the Santa Cruz Mountains reflected shortening that is consistent with movement along reverse faults in the region and with the hypothesis that tectonic strain is distributed widely across numerous faults in the California Coast Ranges.

  14. THE OLYMPIC VILLAGE CLARA NUNES AND IMPASSES AND POSSIBILITIES OF LEISURE AND SPORT PUBLIC POLICY AIMED AT YOUNG PEOPLE FROM POOR AREAS OF THE CITY OF RIO DE JANEIRO

    OpenAIRE

    CAMILA LEITE DA SILVA

    2015-01-01

    A presente dissertação tem por objetivo analisar a trajetória recente das políticas públicas de esporte e lazer voltadas para a população pobre da cidade do Rio de Janeiro. Neste caso, o estudo centraliza sua análise na formulação do projeto socioesportivo Vila Olímpica Clara Nunes (VOCN) localizado em Acari, e procurará conhecer as possibilidades e os desafios para implementação dessa política na garantia dos direitos da população, sobretudo o segmento infanto-juvenil de um dos bairros mais ...

  15. Análisis espacial del diagnóstico tardío vs. mortalidad: una herramienta para apoyar estudios epidemiológicos sobre el cáncer. Villa Clara. Cuba 2004-2009

    Directory of Open Access Journals (Sweden)

    Norma E. Batista-Hernández

    2012-01-01

    Full Text Available Fundamento: la tasa de mortalidad por cáncer en Villa Clara, al cierre del 2009, se mantuvo por encima de la media nacional, con ocurrencia significativa de mayor número de casos con diagnóstico tardío en fallecidos que en los sobrevivientes al período de estudio. Objetivo: mostrar las potencialidades del análisis espacio-temporal para identificar conglomerados espaciales y/o espacio-temporales que nos permitan el análisis del diagnóstico tardío como variable explicativa de la mortalidad, de las cinco localizaciones de cánceres bajo programa en la provincia. Métodos: estudio observacional descriptivo, focalizado en la detección de conglomerados espaciales y espacio-temporales del diagnostico tardío del cáncer en Villa Clara, utilizando una técnica estadística de exploración espacio-temporal (SaTScan v7.0.1., la muestra abarcó la totalidad de pacientes diagnosticados durante el año 2004, y de estos, los fallecidos hasta el cierre del año 2009. Resultados: reveló la presencia de conglomerados significativos tanto espaciales como espacio-temporales, de las áreas de mayor riesgo de diagnóstico indeterminado. Conclusiones: demostró ser una buena herramienta para el análisis del diagnóstico tardío del cáncer, y permitió la generación de hipótesis sobre posibles determinantes que ayuden a orientar nuevas investigaciones.

  16. Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans

    Directory of Open Access Journals (Sweden)

    Fei Yin

    2017-11-01

    Full Text Available Abstract Background False kelpfish (Sebastiscus marmoratus is one of the target species in artificial breeding in China, and is susceptible to infection by Cryptocaryon irritans, which is an obligate parasitic ciliate that lives in the epithelium of the fish gills, skin and fins. Here, we sought to understand the mechanisms of molecular immunity of S. marmoratus against C. irritans infection. Methods We carried out an extensive analysis of the transcriptome of S. marmoratus immune-related tissues. A paired-end library was constructed from the cDNA synthesized using a Genomic Sample Prep Kit. Five normalized cDNA libraries were constructed using RNA from the control group and the four groups of C. irritans-infected fish. The libraries were sequenced on an Illumina Mi-Seq platform, and functional annotation of the transcriptome was performed using bioinformatics software. Results The data produced a total of 149,983,397 clean reads from five cDNA libraries constructed from S. marmoratus immune-related tissues. A total of 33,291 unigenes were assembled with an average length of 1768 bp. In eggNOG (Evolutionary Genealogy of Genes: non-supervised orthologous groups categories, 333 unigenes (0.94% were assigned to defense mechanisms. In the immune system process sub-categories of gene ontology (GO enrichment analysis, with the passage of time post-infection, the number of differentially expressed genes (DEGs was reduced from 24 h to 48 h but then increased from 72 h to 96 h. Specifically, the immune-related differentially expressed genes (IRDEGs, which belong to the KEGG (Kyoto encyclopedia of genes and genomes pathways, such as the complement and coagulation cascades, chemokine signalling pathways and toll-like receptor signalling pathways were mainly observed at 24 h post-infection. Conclusions Infection with C. irritans resulted in a large number of DEGs in the immune-related tissues of S. marmoratus. The rapid and significant response of the

  17. Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans.

    Science.gov (United States)

    Yin, Fei; Qian, Dong

    2017-11-21

    False kelpfish (Sebastiscus marmoratus) is one of the target species in artificial breeding in China, and is susceptible to infection by Cryptocaryon irritans, which is an obligate parasitic ciliate that lives in the epithelium of the fish gills, skin and fins. Here, we sought to understand the mechanisms of molecular immunity of S. marmoratus against C. irritans infection. We carried out an extensive analysis of the transcriptome of S. marmoratus immune-related tissues. A paired-end library was constructed from the cDNA synthesized using a Genomic Sample Prep Kit. Five normalized cDNA libraries were constructed using RNA from the control group and the four groups of C. irritans-infected fish. The libraries were sequenced on an Illumina Mi-Seq platform, and functional annotation of the transcriptome was performed using bioinformatics software. The data produced a total of 149,983,397 clean reads from five cDNA libraries constructed from S. marmoratus immune-related tissues. A total of 33,291 unigenes were assembled with an average length of 1768 bp. In eggNOG (Evolutionary Genealogy of Genes: non-supervised orthologous groups) categories, 333 unigenes (0.94%) were assigned to defense mechanisms. In the immune system process sub-categories of gene ontology (GO) enrichment analysis, with the passage of time post-infection, the number of differentially expressed genes (DEGs) was reduced from 24 h to 48 h but then increased from 72 h to 96 h. Specifically, the immune-related differentially expressed genes (IRDEGs), which belong to the KEGG (Kyoto encyclopedia of genes and genomes) pathways, such as the complement and coagulation cascades, chemokine signalling pathways and toll-like receptor signalling pathways were mainly observed at 24 h post-infection. Infection with C. irritans resulted in a large number of DEGs in the immune-related tissues of S. marmoratus. The rapid and significant response of the S. marmoratus immune signalling pathways following C

  18. Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker (Larimichthys crocea) to ciliate ectoparasite Cryptocaryon irritans infection.

    Science.gov (United States)

    Yin, Fei; Gao, Quanxin; Tang, Baojun; Sun, Peng; Han, Kunhuang; Huang, Weiqing

    2016-03-01

    Large yellow croaker (Larimichthys crocea) is one of the most valuable marine fish in southern China. Given to the rapid development of aquaculture industry, the L. crocea was subjected to ciliate ectoparasite Cryptocaryon irritans. It therefore is indispensable and urgent to understand the mechanism of L. crocea host defense against C. irritans infection. In the present study, the extensively analysis at the transcriptome level for Cryptocaryoniasis in L. crocea was carried out. These results showed that 15,826,911, 16,462,921, and 15,625,433 paired-end clean reads were obtained from three cDNA libraries (A: 0 theronts/fish, B: 12,000 theronts/fish, and C: 24,000 theronts/fish) of the L. crocea immune-related tissues by Illumina paired-end sequencing technology. Totally, 30,509 unique transcript fragments (unigenes) were assembled, with an average length of 1715 bp. In B/A, C/A, and C/B pairwise comparison, 972, 900, and 1126 genes showed differential expression respectively. Differently expressed immune-related genes (DEIGs) were scrutinized, in B/A pairwise comparison, 48 genes showed differential expression, including 26 up-regulated genes and 22 down-regulated genes in B; in C/A pairwise comparison, there were 39 DEIGs, including 7 up-regulated genes and 32 down-regulated genes in C; in C/B pairwise comparison, 40 genes showed differential expression, including 11 up-regulated genes and 29 down-regulated genes in C. There were 16 DEIGs enriched KEGG pathways, in which the complement and coagulation cascades pathway was the top most DEIGs enriched pathway (B:A = 42; C:A = 28; C:B = 42). The coagulation and fibrinolytic system was in a highly active state after infected by C. irritans with non-lethal concentration; the alternative complement pathway may play an important role in the early stages of C. irritans infection. These results demonstrated that low-concentration infection can significantly induce the immunological response in fishes, however, when fishes

  19. Viscosity of egg white from hens of different strains fed with commercial and natural additives Viscosidade da clara de ovo proveniente de poedeiras de diferentes espécies com aditivos comerciais e naturais

    Directory of Open Access Journals (Sweden)

    Fernanda Papa Spada

    2012-03-01

    Full Text Available Yolk color and egg white (albumen cleanliness and viscosity are important parameters by which consumers judge the quality of eggs. This study aimed to investigate changes in albumen viscosity during storage of eggs for up to 36 days from two different commercial laying hen strains (Carijo Barbada and Isa Brown fed a diet containing annatto (1.5 and 2.0% or a synthetic additive without synthetic colorants (control. Analyses of humidity, albumen height, pH, viscosity, foam formation, and stability were carried out on eggs. Carijo Barbada strain had smaller albumen, lower humidity and higher egg white viscosity than Isa Brown strain; however, with storage, viscosity lowered significantly on both strains. Initially, the addition of 2.0% of annatto or a synthetic additive increased viscosity in both strains, but with storage only the control maintained longer viscosity. Lower viscosity did not change foam density and stability.A coloração da gema, transparência e viscosidade da clara (albúmen são parâmetros importantes que os consumidores consideram na avaliação dos ovos. O objetivo do estudo foi verificar as trocas que ocorrem na armazenagem por 36 dias em relação à viscosidade da clara de ovos produzidos por poedeiras de duas diferentes linhagens (Carijo Barbada eIsa Brown , as quais receberam dietas contendo urucum (1,5 e 2,0% ou aditivo sintético. Foram realizadas as análises de umidade, altura do albúmen, pH, viscosidade, formação e estabilidade da espuma. A linhagem Carijó Barbada apresentou menor quantidade de albúmen, menor umidade e maior viscosidade que a linhagem Isa Brown, entretanto, com a estocagem, a viscosidade diminuiu significativamente para ambas as linhagens. Inicialmente, a adição de 2,0% de urucum ou aditivo sintético foi suficiente para aumentar a viscosidade em ovos obtidos de ambas as linhagens, mas com a estocagem, somente o controle manteve maior viscosidade. A baixa viscosidade não interferiu na

  20. Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii

    Directory of Open Access Journals (Sweden)

    Adriana Vallesi

    2013-04-01

    Full Text Available In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation.

  1. Predação e canibalismo entre protozoários ciliados (Ciliophora: Entodiniomorphida: Ophryoscolecidae no rúmen de ovinos (Ovis aries Predation and cannibalism among ciliate protozoans (Ciliophora: Entodiniomorphida: Ophryoscolecidae in the rumen of sheep (Ovis aries

    Directory of Open Access Journals (Sweden)

    Isabel Martinele

    2008-09-01

    Full Text Available O objetivo deste trabalho foi registrar ocorrências de predação e canibalismo em protozoários ciliados no rúmen de ovinos. Foram analisadas amostras de conteúdo ruminal de cinco ovinos mestiços Santa Inês, fistulados no rúmen, mantidos em pastagem natural de caatinga, no município de Sertânia, Pernambuco. Foram obtidas seis amostras de conteúdo ruminal de cada ovino, totalizando 30 amostras, as quais foram fixadas em formalina 18,5% (v/v. De cada amostra foi retirada uma alíquota de 1 ml de conteúdo ruminal ao qual se adicionaram três gotas de solução de lugol para a coloração e identificação dos protozoários ciliados. Estas alíquotas foram analisadas utilizando-se lâminas e lamínulas, em campos determinados randomicamente. Constatou-se a ocorrência de interações do tipo predador-presa entre ciliados de Ophryoscolecidae Stein, 1859, onde Elytroplastron bubali (Dogiel, 1928 apresentou comportamento predatório sobre Enoploplastron triloricatum (Dogiel, 1925 e sobre outras espécies de Epidinium Crawley, 1923 e de Entodinium Stein, 1859. Foram ainda observados casos de canibalismo entre espécimes de E. bubali, o que consiste no primeiro registro deste tipo de interação nesta espécie. Registra-se, ainda, pela primeira vez a ocorrência de E. bubali no rúmen de ovinos no Brasil.The objective of the present study was to record the occurrence of predation and cannibalism among ciliate protozoans in the rumen of sheep. The analyzed samples consisted of ruminal contents of five fistulated Santa Inez crossbred sheep, maintained in a natural caatinga (scrubland pasture in the municipality of Sertânia, Pernambuco. Six samples of ruminal content were obtained from each sheep (total of 30 samples and fixed in formalin at 18.5% (v/v. One 1 ml aliquot was obtained from each sample of ruminal content and received tree drops of a lugol solution for staining and identification of protozoa ciliates. These aliquots were analyzed in

  2. Esker Ri Nursing Home, Kilnabinnia, Clara, Offaly.

    LENUS (Irish Health Repository)

    Kennedy, Harry G

    2011-09-01

    This series of rating \\'ladders\\' is intended to allow a quantitative and qualitative analysis of the use of restrictive and intrusive interventions as part of the therapeutic management of violence and aggression in psychiatric hospital settings. This is an evolving handbook. The ladders are currently organised to facilitate a behavioural analysis. Context, antecedents, behaviour, interventions, consequences are conceptualised as a series of events organised in temporal sequence so that causes, interactions and effects can be considered. The complexity of analysis possible is limited by the statistical power of the numbers of cases and events available. \\r\

  3. DCS Hydrology, Santa Clara County, California

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

  4. Esker Ri Nursing Home, Kilnabinnia, Clara, Offaly.

    LENUS (Irish Health Repository)

    Sheehan, Aisling

    2015-02-01

    Nursing is experiencing a significant deficit in research capacity needed to meet future global healthcare demands-there is a call to double the number of nurses and healthcare professionals with a doctorate.

  5. Floodplain Mapping, SANTA CLARA COUNTY, CALIFORNIA, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  6. River channel sensitivity to change in the context of human activities and natural factors: an 80-year record of channel morphodynamics on the lower Santa Clara River, Ventura County, California

    Science.gov (United States)

    Downs, P. W.; Dusterhoff, S. R.; Sears, W. A.

    2010-12-01

    River channel adjustments arise from the application of numerous catchment-based stressors operating at different space and time scales. Natural stressors include the impact of climatic phenomena and their inheritance; human stressors include both direct and indirect factors whose impacts have grown in magnitude and intensity during the Anthropocene, especially since about 1945. Consequently, the sensitivity of river channel morphodynamics is likely to have changed also, with implications for landform understanding and river management. Reconstructing channel morphodynamics during the Anthropocene requires interpreting multiple historical and secondary data sources to document changes at sufficient (i.e., reach-scale) resolution: for the 60-km lower Santa Clara River (LSCR), Ventura County, California, we used flow, sediment and precipitation records, repeat aerial photographs, LiDAR data, repeat topographic surveys, in-channel vegetation data, field observations, numerical modeling of high flow events, and narrative accounts. The catchment historical context since European-American settlement includes periods dominated by ranching and colonization (ca.1820-1890), irrigations and diversions (ca.1890-1955), dams and river modifications (1955-1990), and urban population growth (1990-present). Natural stressors were investigated based on the correlation of instantaneous flood peaks with annual rainfall records in this semi-arid setting. Successful prediction of the majority of gauged floods since about 1950 allows a flood sequence to be reconstructed back to 1873. Floods are clustered and of considerably greater magnitude in El Nino years of the El Nino-Southern Oscillation. The great majority of sediment transport thus occurs in El Nino years so that the dominant discharge is the largest discharge on record, in contrast to humid-region alluvial rivers. Responding to these stressors, the average width of the active channel bed has become narrower by almost 50% (1938

  7. Streamflow gains and losses along San Francisquito Creek and characterization of surface-water and ground-water quality, southern San Mateo and northern Santa Clara counties, California, 1996-97

    Science.gov (United States)

    Metzger, Loren F.

    2002-01-01

    San Francisquito Creek is an important source of recharge to the 22-square-mile San Francisquito Creek alluvial fan ground-water subbasin in the southern San Mateo and northern Santa Clara Counties of California. Ground water supplies as much as 20 percent of the water to some area communities. Local residents are concerned that infiltration and consequently ground-water recharge would be reduced if additional flood-control measures are implemented along San Francisquito Creek. To improve the understanding of the surface-water/ground-water interaction between San Francisquito Creek and the San Francisquito Creek alluvial fan, the U.S. Geological Survey (USGS) estimated streamflow gains and losses along San Francisquito Creek and determined the chemical quality and isotopic composition of surface and ground water in the study area.Streamflow was measured at 13 temporary streamflow-measurement stations to determine streamflow gains and losses along a 8.4-mile section of San Francisquito Creek. A series of five seepage runs between April 1996 and May 1997 indicate that losses in San Francisquito Creek were negligible until it crossed the Pulgas Fault at Sand Hill Road. Streamflow losses increased between Sand Hill Road and Middlefield Road where the alluvial deposits are predominantly coarse-grained and the water table is below the bottom of the channel. The greatest streamflow losses were measured along a 1.8-mile section of the creek between the San Mateo Drive bike bridge and Middlefield Road; average losses between San Mateo Drive and Alma Street and from there to Middlefield Road were 3.1 and 2.5 acre-feet per day, respectively.Downstream from Middlefield Road, streamflow gains and losses owing to seepage may be masked by urban runoff, changes in bank storage, and tidal effects from San Francisco Bay. Streamflow gains measured between Middlefield Road and the 1200 block of Woodland Avenue may be attributable to urban runoff and (or) ground-water inflow. Water

  8. Acesso ao alimento artificial e enchimento do trato digestivo de juvenis do camarão marinho Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae durante as fases clara e escura do período de 24 horas Artificial food access and digestive tract filling of juvenil marine shrimp Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae during light and dark phases in 24-hour period

    Directory of Open Access Journals (Sweden)

    Cibele S. Pontes

    2005-12-01

    Full Text Available A escassez de dados acerca do comportamento alimentar do camarão marinho Litopenaeus vannamei (Boone, 1931 com relação ao alimento artificial ofertado em bandejas poderá induzir a uma alimentação inadequada, resultando no desperdício da ração, no aporte desnecessário de nutrientes, e conseqüente aumento dos custos com a alimentação e da poluição ambiental potencial desta atividade. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram realizados estudos comportamentais utilizando 64 juvenis de Litopenaeus vannamei (7,57 ± 1,01g, mantidos em densidade populacional de 33 m², submetidos a fotoperíodo artificial, em ciclo invertido, para observação durante as fases clara e escura. A ração foi ofertada em intervalos pré-estabelecidos, registrando-se posteriormente através de método focal contínuo: a latência para chegada à bandeja, b latência para consumo do alimento e c Índice de Enchimento do Trato Digestivo. Os animais foram mais rápidos para chegar à bandeja e também para iniciar o consumo do alimento nos horários da fase clara. O enchimento do trato digestivo mostrou-se superior na meia hora subsequente à oferta do alimento, especialmente nos horários da fase clara.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to feed offer in trays can result inadequate feeding by the animal, feed wastage and unnecessary nutrient input, increasing feed costs and potential environmental pollution of that culture. In order to provide tools for improved feed management methods in shrimp farms, a behavioral study was conducted, using 64 Litopenaeus vannamei juveniles (7,57 ± 1,01g, one animal per 33 m². They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register behavior during light and dark phases. The following variables were registered (continuous sampling after feed exposition: a latency to access the

  9. Mineralogy and trace-element geochemistry of the high-grade iron ores of the Águas Claras Mine and comparison with the Capão Xavier and Tamanduá iron ore deposits, Quadrilátero Ferrífero, Brazil

    Science.gov (United States)

    Spier, Carlos Alberto; de Oliveira, Sonia Maria Barros; Rosière, Carlos Alberto; Ardisson, José Domingos

    2008-02-01

    Several major iron deposits occur in the Quadrilátero Ferrífero (QF), southeastern region of Brazil, where metamorphosed and heterogeneously deformed banded iron formation (BIF) of the Cauê Formation, regionally called itabirite, was transformed into high- (Fe >64%) and low-grade (30% mines in the QF, the Águas Claras Mine contained mainly high-grade ores hosted within dolomitic itabirite. Two distinct types of high-grade ore occurred at the mine: soft and hard. The soft ore was the most abundant and represented more than 85% of the total ore mined until it was mined out in 2002. Soft and hard ores consist essentially of hematite, occurring as martite, anhedral to granular/tabular hematite and, locally, specularite. Gangue minerals are rare, consisting of dolomite, sericite, chlorite, and apatite in the hard and soft ores, and Mn-oxides and ferrihydrite in the soft ore where they are concentrated within porous bands. Chemical analyses show that hard and soft ores consist almost entirely of Fe2O3, with a higher amount of detrimental impurities, especially MnO, in the soft ore. Both hard and soft ores are depleted in trace elements. The high-grade ores at the Águas Claras Mine have at least a dual origin, involving hypogene and supergene processes. The occurrence of the hard, massive high-grade ore within “fresh” dolomitic itabirite is evidence of its hypogene origin. Despite the contention about the origin of the dolomitic itabirite (if this rock is a carbonate-rich facies of the Cauê Formation or a hematite-carbonate precursor of the soft high-grade ore), mineralogical and geochemical features of the soft high-grade ore indicate that it was formed by leaching of dolomite from the dolomitic itabirite by meteoric water. The comparison of the Águas Claras, Capão Xavier and Tamanduá orebodies shows that the original composition of the itabiritic protore plays a major role in the genesis of high- and low-grade soft ores in the QF. Under the same weathering

  10. Ciliates as engineers of phototrophic biofilms.

    NARCIS (Netherlands)

    Weerman, E.J.; van der Geest, H.G.; van der Meulen, M.D; Manders, E.M.M.; van de Koppel, J.; Herman, P.M.J.; Admiraal, W.

    2011-01-01

    1. Phototrophic biofilms consist of a matrix of phototrophs, non-photosynthetic bacteria and extracellular polymeric substances (EPS) which is spatially structured. Despite widespread exploitation of algae and bacteria within phototrophic biofilms, for example by protozoans, the ‘engineering’

  11. Ciliates as engineers of phototrophic biofilms

    NARCIS (Netherlands)

    Weerman, Ellen J.; van der Geest, Harm G.; van der Meulen, Myra D.; Manders, Erik M. M.; van de Koppel, Johan; Herman, Peter M. J.; Admiraal, Wim

    1. Phototrophic biofilms consist of a matrix of phototrophs, non-photosynthetic bacteria and extracellular polymeric substances (EPS) which is spatially structured. Despite widespread exploitation of algae and bacteria within phototrophic biofilms, for example by protozoans, the 'engineering'

  12. Ciliates as engineers of phototrophic biofilms.

    NARCIS (Netherlands)

    Weerman, E.J.; Geest, H.G.; Meulen, M.D.; Manders, E.M.M.; Van de Koppel, J.; Herman, P.M.J.; Admiraal, W.

    2011-01-01

    1.Phototrophic biofilms consist of a matrix of phototrophs, non-photosynthetic bacteria and extracellular polymeric substances (EPS) which is spatially structured. Despite widespread exploitation of algae and bacteria within phototrophic biofilms, for example by protozoans, the ‘engineering’ effects

  13. Rumen ciliate protozoa in sheep raising in the caatinga region of Pernambuco State, Brazil População de protozoários ciliados no rúmen de ovinos criados na caatinga de Pernambuco

    Directory of Open Access Journals (Sweden)

    Gladston Rafael de Arruda Santos

    2008-06-01

    Full Text Available This work sought to determine the number and genus of rumen ciliate protozoa in caatinga sheep raising, before and four hours after feeding them. There were collected 10 mL of ruminal fluid which was fixed in 10 mL of 10% formalin. After counting and identification of genera, the variables were submitted to variance analysis, the means compared by Tukey test and accomplished of simple correlation test between protozoa and chemical composition diet. Among found genera there was predominance of Entodinium (around 90%. Holotricha subclass only Isotricha genus was registered which represented 1.94% of population. The percentage of protozoa only varied (P <0.05 for Eodinium genus. It’s highest variation was showed in May 2005. Related to the collection schedule, only the pH, the medium number of Entodinium gender and total number of protozoa presented significant difference, being larger before feeding the animals. Other genera also presented the same behavior, thus reflecting the stability of ruminal environment for animals without access to night feeding. The protozoa of Entodiniomorphid subclass present high positive correlation with level of total digestible nutrients and crude protein of the diet. This reflects the necessity, for microorganism, of energy and protein for synthesis (cellular division and storage.Objetivou-se com este trabalho determinar o número e gênero de protozoários ciliados no rúmen de ovinos criados na caatinga antes da alimentação e quatro horas após. Foram coletados 10 mL de fluido ruminal, fixados em 10 mL de formalina a 10 %. Após contagem e identificação dos gêneros, as variáveis foram submetidas à análise de variância, as médias comparadas pelo teste de Tukey, sendo, ainda, realizado o teste de correlação simples entre protozoários e a composição química da dieta. Dentre os gêneros encontrados, houve predominância do gênero Entodinium (em torno de 90%. Da subclasse Holotricha, foi registrado

  14. Comportamento de Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae em função da oferta do alimento artificial nas fases clara e escura do período de 24 horas Behavior of Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae in relation to artificial food offer along light and dark phases in a 24 h period

    Directory of Open Access Journals (Sweden)

    Cibele S. Pontes

    2005-09-01

    Full Text Available A escassez de dados acerca do comportamento do camarão marinho Litopenaeus vannamei (Boone, 1931 relacionado ao alimento artificial ofertado em comedouros poderá induzir a uma alimentação inadequada, aumentando a relação custo/benefício e os impactos ambientais potenciais do seu cultivo. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram desenvolvidos estudos comportamentais utilizando 64 juvenis de L. vannamei (7,57 ± 1,01 g, submetidos a fotoperíodo artificial, metade deles em ciclo invertido, para observação das suas atividades durante as fases clara e escura. A ração foi oferecida em intervalos pré-estabelecidos, registrando-se antes e depois da oferta: exploração, natação, alimentação e inatividade (focal instantâneo. A natação ocorreu preferencialmente durante a fase escura. A alimentação foi mais elevada na primeira meia hora posterior à oferta, com ênfase nos horários da fase clara. O alimento artificial atuou, em ambas as fases, como um indutor da exploração do substrato, tornando os camarões mais ativos em todos os horários posteriores à sua oferta.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to artificial food offer in feeding trays may result an inadequate feeding by the animal, increasing the cost/benefit relation in shrimp culture and its potential environmental impact. In order to provide tools for optimizing feeding management in shrimp farms, a behavioral study was developed, using 64 L. vannamei juveniles (7,57 ± 1,01 g. They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register their behavior during light and dark phases. In established intervals, the following activities were registered before and after food exposition: exploration, swimming, feeding and inactivity (instantaneous sampling. Swimming occurred mostly during the dark phase. Feeding time was higher in

  15. Fontes de lipídeos e monensina na alimentação de novilhos Nelore e sua relação com a população de protozoários ciliados do rúmen Effects of feeding monensin and different sources of fat on the ruminal population of ciliate protozoa in Nellore steers

    Directory of Open Access Journals (Sweden)

    Amaury Camilo Valinote

    2005-08-01

    Full Text Available Quatro novilhos Nelore, fistulados e canulados no rúmen, foram distribuídos em um delineamento quadrado latino 4 x 4, para avaliar o caroço de algodão e o sal de cálcio de ácidos graxos como fontes de gordura assim como o efeito da monensina em dietas com caroço de algodão, sobre a população de protozoários ciliados e o pH do rúmen. Os tratamentos experimentais foram: dieta controle (CTRL, dieta com sal de cálcio de ácidos graxos (SC, dieta com caroço de algodão (CA e dieta com caroço de algodão sem monensina (CASM. Amostras do conteúdo ruminal foram colhidas às 0, 2, 4, 6 e 8 horas após a alimentação. Foi utilizada câmara de contagem de Sedgwick-Rafter com capacidade de 1mL e microscópio ótico comum provido de retículo com área de 0,4362 mm², para identificação dos gêneros de ciliados. Não houve diferença entre os tratamentos para o pH do rúmen. Os tratamentos com caroço de algodão reduziram todos os gêneros de protozoários ciliados, em especial de Entodinium, provavelmente pela gordura liberada. Os protozoários dos gêneros Diplodinium, Epidinium e Eudiplodinum e o número total de protozoários foram os únicos que sofreram influência do tempo de alimentação. Sais de cálcio de ácidos graxos foram suficientemente inertes no ambiente ruminal, não afetando o número de protozoários ciliados no rúmen de bovinos Nelore. A utilização de monensina em dietas contendo caroço de algodão e alto concentrado não influenciou o número de protozoários ciliados no rúmen de bovinos Nelore.Four ruminally and duodenally cannulated Nellore steers were assigned to a 4 x 4 latin square to study the effects of feeding monensin (M and different sources of fat on the ruminal population of ciliate protozoa and ruminal pH. The experimental diets contained (DM basis 19% of sugar cane and were supplemented with M [control (CTRL], M plus calcium salts of fatty acids (CSFA, M plus whole cottonseed (MWCS, or only WCS

  16. Níveis de proteína degradável para novilhas em crescimento sobre a concentração de protozoários ciliados e outros parâmetros ruminais Levels of degradable protein given to heifers for the ciliate protozoa concentration and other ruminal parameters

    Directory of Open Access Journals (Sweden)

    Joaquim Balcells

    2001-05-01

    Full Text Available Em um ensaio de controle de parâmetros ruminais (pH, ritmo de trânsito (%h, volume ruminal (l, e concentração de protozoários ciliados, foram utilizadas quatro novilhas Holstein, canuladas no rúmen e duodeno, recebendo dietas com 4 níveis de proteína degradável, sem suplementar ou suplementadas com 25, 50 e 75 gramos de proteína degradável por quilo de concentrado em forma de uréia e caseína (98,2 e 93,2% de matéria orgânica e proteína bruta, respectivamente. As rações experimentais foram compostas por 25% de palha de cevada sem tratar e 75% de concentrado formulado em base a diferentes proporções (25/75 e 75/25 de milho/cevada, com 5% de resíduo de trigo e 5% de suplemento vitamínico mineral. Uma contínua infusão de marcador de fluxo de fase líquida (Cr-EDTA foi executada. Os oito tratamentos experimentais (2 dietas x 4 níveis de infusão foram administrados em 8 períodos de balanço em um delineamento cross-over, em esquema fatorial. O nível de infusão de N degradável afetou de forma significativa os valores médios ponderados de pH, que decresceram ao aumentar a suplementação (p 3/mL que de cevada (59,5 x 103/mL; p Entodinium alcançou 87,8% da população total. O gênero Epidinium apresentou menores valores com a dieta de cevada (3,73 vs. 1,89x103/mL, p 3/mL, p 5/mL, p In this experiment four Holstein heifers fitted with rumen and duodenum canulae were utilized to evaluate the rumen parameters as pH, passage rate (%/h, volume (L and ciliated protozoa concentration, when fed diets with four levels of rumen degradable protein. This was achieved supplementing 0, 25, 50 or 75 grams of degradable protein per kg of concentrate as a urea and casein (98.2% OM and 93.2% CP. The diets had 25% of barley straw without treatment and 75% concentrate formulated to contain different proportions (25/75 and 75/25 of corn and barley and also 5% of wheat residue and 5% of mineral and vitamin supplement. A continuous infusion of

  17. Abundância de ciliados planctônicos em reservatórios em cascata do rio Paranapanema, Brasil- DOI: 10.4025/actascibiolsci.v26i4.1532 Abundance of planktonic ciliates in a cascading reservoirs of the Paranapanema River, Brazil - DOI: 10.4025/actascibiolsci.v26i4.1532

    Directory of Open Access Journals (Sweden)

    Maysa Cláudia Zolin Perenha

    2004-04-01

    Full Text Available O presente estudo teve como objetivo investigar os padrões de distribuição espacial e temporal da densidade e biomassa dos ciliados planctônicos em reservatórios em cascata do rio Paranapanema (22o 18’ - 23o 35’ S; 49o 30’ - 52o 50’ W. Pretende-se testar a hipótese de que, ao longo da série de reservatórios, observa-se um decréscimo da abundância desses organismos no sentido montante-jusante. Para tal, foram coletadas amostras em sete reservatórios, no ano de 2001, em dois períodos hidrológicos distintos, à sub-superfície da região lacustre, com o auxílio de uma garrafa de Van Dorn de 5 litros. Alíquotas de um litro foram obtidas e sua fixação realizada com solução fixadora composta de lugol alcalino, formol e tiossulfeto de sódio. Os valores obtidos para a abundância (densidade e biomassa de ciliados nos diferentes reservatórios (0,4 a 16,6 células.mL-1; 0,23 a 20,7 µg.C.L-1, respectivamente foram inferiores aos resultados encontrados em ambientes eutróficos, porém similares àqueles registrados em ambientes oligo-mesotróficos. A análise dos resultados de distribuição da abundância ao longo da série de reservatórios não corroborou a hipótese preconizada de uma diminuição de densidade e biomassa no sentido montante-jusante, em função da expectativa de uma possível oligotrofização rio abaixo. Em relação aos diferentes grupos funcionais de ciliados, foi constatada a dominância dos Oligotrichida, principalmente os de menor porte (The present study aimed to investigate the spatial and temporal distribution patterns of density and biomass of the planktonic ciliates, in a reservoir serie of the Paranapanema River (22o 18’ - 23o 35’ S; 49o 30’ - 52o 50’ W. We intend to test the hypothesis that, along reservoirs serie, there is a decrease of abundance from river towards the dam. Samplings were carried out in seven reservoirs, during 2001, in two distinct hydrological periods, at subsurface

  18. Protozoários ciliados do rúmen de bovinos e bubalinos alimentados com dietas suplementadas com monensina ou própolis Ruminal ciliate protozoa of cattle and buffalo fed on diet supplemented with monensin or extracts from propolis

    Directory of Open Access Journals (Sweden)

    Thaís Barros Ríspoli

    2009-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de dietas suplementadas com monensina ou produtos à base de própolis, nas populações de protozoários ciliados no rúmen de bovinos (Bos taurus e bubalinos (Bubalus bubalis. Quatro bovinos da raça Holandesa e quatro búfalos da raça Murrah adultos, fistulados no rúmen, foram distribuídos em delineamento quadrado latino (4x4. A dieta constituiu-se de 50% de silagem de milho e 50% de concentrado à base de milho em grãos e farelo de soja, com adição de monensina sódica ou aditivo à base de própolis LLOSA2 ou LLOSC1. As amostras do conteúdo ruminal foram coletadas duas horas após a alimentação. O gênero Entodinium foi o mais representativo em todos os tratamentos, para ambas as raças de ruminantes. Em búfalos, foi observado o efeito redutor do tratamento LLOSC1 nas populações do gênero Entodinium, além do efeito redutor dos tratamentos monensina e LLOSA2 sobre os gêneros da subfamília Diplodiniinae. A média de ciliados foi maior em bubalinos (56x10(4 mL-1 do que em bovinos (26x10(4 mL-1. Houve aumento do pH ruminal dos bovinos no tratamento com monensina. O extrato de própolis LLOSC1 reduziu os ciliados do rúmen em bubalinos.The aim of this work was to evaluate the effect of diets supplemented with monensin or with extracts of propolis, on the population of ciliate protozoa in the rumen of cattle (Bos taurus and water buffalo (Bubalus bubalis. Four Holstein cattle and four Murrah buffalo, fistulated in the rumen, were distributed in 4x4 Latin square design. The diet was constituted by 50% corn silage and 50% concentrate based on corn grain and soybean meal, and the supplementation of monensin sodium or propolis extracts LLOSA2 or LLOSC1. Samples of ruminal contents were collected two hours after feeding. The genus Entodinium was the most common in all treatments for both cattle and buffalo. The LLOSC1 treatment reduced the populations of the genus Entodinium in buffalo

  19. Una nota sobre escultura castellana del siglo XIII: Juan González, el pintor de las imágenes de Burgos, y el sepulcro de doña Mayor Guillén de Guzmán en el convento de Santa Clara de Alcocer (Guadalajara

    Directory of Open Access Journals (Sweden)

    Gutiérrez Baños, Fernando

    2015-03-01

    Full Text Available In 2009 Christie’s London brought to light the contract for the tomb of doña Mayor Guillén de Guzmán († c. 1263, former mistress of king Alfonso X the Wise. The contract comes from the convent of Poor Clares of Alcocer (Guadalajara, where the tomb itself remained until completely destroyed in 1936 at the beginning of the Spanish Civil War. Dated in Burgos in 1276, the document provides a full description of the work to be carried out. This allows us to compare it with other contemporary tombs, underlining the highly innovative features of that created for doña Mayor. In addition, the document provides access to the personality of the artist responsible for this work: a certain until now unknown Juan González, who introduces himself as el pintor de las imágenes de Burgos (‘the painter of images of Burgos’.En 2009 Christie’s de Londres dio a conocer el contrato del sepulcro de doña Mayor Guillén de Guzmán († c. 1263, antigua amante del rey Alfonso X el Sabio. El contrato procede del convento de Santa Clara de Alcocer (Guadalajara, donde el propio sepulcro se conservó hasta su completa destrucción en 1936 al estallar la Guerra Civil. Datado en Burgos en 1276, el documento nos proporciona una completa descripción de la obra a realizar. Esto nos permitirá compararla con otros sepulcros contemporáneos, subrayando los rasgos altamente innovadores del sepulcro de doña Mayor. Por otra parte, el documento nos permitirá acceder a la personalidad del artista responsable de la obra: un cierto Juan González, desconocido hasta la fecha, que se presenta como el pintor de las imágenes de Burgos.

  20. 77 FR 39726 - Land Acquisitions: Pueblo of Santa Clara

    Science.gov (United States)

    2012-07-05

    ... Office, Bureau of Indian Affairs, 1001 Indian School Road NW., Albuquerque, NM 87104-2303; Telephone (505... N., R. 7 E., Sec. 17, lots 1 to 8, inclusive; Sec. 18, lots 5 to 12, inclusive; Sec. 19, lots 12 to 17, inclusive, lots 19, 21, 23, all that portion of Tract 37 within sec. 19, all that portion of...

  1. 78 FR 66982 - Santa Clara Pueblo Disaster #NM-00039

    Science.gov (United States)

    2013-11-07

    ... Available 2.875 Elsewhere For Economic Injury: Non-Profit Organizations Without Credit Available 2.875... Deadline Date: 12/30/2013. Economic Injury (EIDL) Loan Application Deadline Date: 07/29/2014. ADDRESSES... major disaster declaration on 10/29/2013, Private Non- Profit organizations that provide essential...

  2. Different Cell Types In the Lower Respiratory Tract of the Reindeer (Rangifer tarandus tarandus L. - A Transmission Electron Microscopical Study

    Directory of Open Access Journals (Sweden)

    Seppo A.m. Saari

    1997-02-01

    Full Text Available The epithelium of the trachea and distal airways of 12 healthy adult reindeer were studied with transmission electron microscopy. The ultrastructure of the reindeer respiratory tract corresponded to the findings of previous investigators studying other mammalian species. The epithelium of the trachea and bronchi, down to the level of the distal bronchioli, was composed of three main types of cell: ciliated, goblet, and basal. In the distal brochioli, non-ciliated cells similar to those known as Clara cells were predominant. Numerous electron-dense granules and the cell organelle pattern resembled the Clara cell type observed in laboratory rodents, rabbit, sheep, pig, horse, and llama. Pneumocyte 1 and pneumocyte 2 cells were readily identified in the alveoli. The pneumocyte 2 cells possessed short microvilli and granules with lamellar content. Micropinocytotic vesicles were very numerous in the alveolar wall, and a small number of alveolar macrophages occasionally seen in the alveolar lumen.

  3. Antioxidant Micronutrients: Therapeutic Counter Measures for Chemical Agents

    Science.gov (United States)

    2011-03-01

    necrosis (AEN), bronchiolar exudates (BE), bronchiolar inflammatory cell infiltrates (BICI), bronchiolar epithelial necrosis (BEN), BALT necrosis (BN...Bronchiolar epithelial necrosis (BEN), BALT necrosis (BN), Tracheal exudates (TE), Tracheal inflammatory cell infiltrates (TICI), Tracheal epithelial...BICI), Bronchiolar epithelial necrosis (BEN), BALT necrosis (BN), Tracheal exudates (TE), Tracheal inflammatory cell infiltrates (TICI), Tracheal

  4. Spatiotemporal quantification of cell dynamics in the lung following influenza virus infection

    Science.gov (United States)

    Yin, Lu; Xu, Shuoyu; Cheng, Jierong; Zheng, Dahai; Limmon, Gino V.; Leung, Nicola H. N.; Rajapakse, Jagath C.; Chow, Vincent T. K.; Chen, Jianzhu; Yu, Hanry

    2013-04-01

    Lung injury caused by influenza virus infection is widespread. Understanding lung damage and repair progression post infection requires quantitative spatiotemporal information on various cell types mapping into the tissue structure. Based on high content images acquired from an automatic slide scanner, we have developed algorithms to quantify cell infiltration in the lung, loss and recovery of Clara cells in the damaged bronchioles and alveolar type II cells (AT2s) in the damaged alveolar areas, and induction of pro-surfactant protein C (pro-SPC)-expressing bronchiolar epithelial cells (SBECs). These quantitative analyses reveal: prolonged immune cell infiltration into the lung that persisted long after the influenza virus was cleared and paralleled with Clara cell recovery; more rapid loss and recovery of Clara cells as compared to AT2s; and two stages of SBECs from Scgb1a1+ to Scgb1a1-. These results provide evidence supporting a new mechanism of alveolar repair where Clara cells give rise to AT2s through the SBEC intermediates and shed light on the understanding of the lung damage and repair process. The approach and algorithms in quantifying cell-level changes in the tissue context (cell-based tissue informatics) to gain mechanistic insights into the damage and repair process can be expanded and adapted in studying other disease models.

  5. Sulfide assimilation by ectosymbionts of the sessile ciliate, Zoothamnium niveum

    DEFF Research Database (Denmark)

    Røy, Hans; Vopel, Kay; Huettel, Marcus

    2009-01-01

    was the free-flowing water although the size of the colonies suggests that they live partly submerged in the diffusive boundary layer. We showed that the filtered volume allows Z. niveum to assimilate sufficient sulfide to sustain the symbiosis at a few micromoles per liter in ambient concentration. Numerical...

  6. Act together - implications of symbioses in aquatic ciliates

    DEFF Research Database (Denmark)

    Dziallas, Claudia; Allgaier, Martin; Monaghan, Michael T.

    2012-01-01

    Mutual interactions in the form of symbioses can increase the fitness of organisms and provide them with the capacity to occupy new ecological niches. The formation of obligate symbioses allows for rapid evolution of new lifeforms including multitrophic consortia. Microbes are important component...

  7. Ciliated hepatic foregut cyst: a rare cystic liver lesion

    African Journals Online (AJOL)

    Adele

    right upper quadrant pain. The pain fluctuated in severity and was controlled with oral analgesic medication. There was no associated jaundice, rigors or weight loss. Apart from minimal right upper quadrant tenderness, examination was unremarkable. Full blood count, liver and renal function tests and clotting profile were ...

  8. A new peritrichous Ciliate as a symphoriont on a Tardigrade

    NARCIS (Netherlands)

    Land, van der J.

    1964-01-01

    INTRODUCTION On 24 September 1962 a number of Tardigrada was obtained from a sample of lichens from the French Alps. The lichens had been collected Figs. 1, 2. Pyxidium tardigradum n. sp. on Hypsibius oberhaeuseri (Doy.). on 27 June 1961 from a rock in the department Haute Savoie and had been

  9. [Regeneration of ciliated cells in the internal ear].

    Science.gov (United States)

    Lefèbvre, P

    2002-01-01

    Hair cells are the mechanotranducer transforming the sound into a bioelectrical signal. Hair cell and supporting cell productions are completed during early embryonic development of the mammalian cochlea. In mammalian, after an injury, no hair cell replacement is observed, as opposed to birds, where regenerative mechanisms produce new sensory cells and restore the auditory function. However, a production of hair cells occurs in the mammalian sensory epithelium. Progenitor cells, isolated from newborn rats, proliferate and differentiate in hair cells and supporting cells. Supernumerary hair cells also arise in the cultured organ of Corti. This model is used to investigate the role of cell cycle regulator molecules and cell-cell interaction. The persistence of sensory cell progenitors in adult mammalian organ of Corti and the understanding of the mechanisms leading to the production of hair cells, in the developing cochlea, open the prospect of hair cell regeneration in the mature inner ear.

  10. Some ciliates from the marine wood boring isopod Sphaeroma treubia

    Digital Repository Service at National Institute of Oceanography (India)

    Santhakumari, V.; Nair, N.B.

    -boring isopod genus Sphaeroma, are described. These were collected from the appendages and body proper of S. terebrans, S. annandalei and S. triste from the southwest coast of India. Legenophrys cochinensis, because of its host specificity and distinct...

  11. Effect of an energy-deficient diet on populations of ciliate protozoans in bovine rumen Efeito da deficiência de energia na dieta sobre a população de protozoários ciliados do rúmen de bovinos

    Directory of Open Access Journals (Sweden)

    P.C. Soares

    2008-02-01

    Full Text Available Ten young rumen-cannulated crossbred steers were randomly divided into two groups: a control group (C; n=4, which was fed a balanced diet for daily weight gain of 900g; and a pronounced energy-deprived group (PED; n=6, receiving 30% less of the required energy for maintenance. After 140 days of these alimentary regimes, rumen fluid and urine samples were collected for biochemical and functional tests, before feeding and at 1, 3, 6, and 9 hours after feeding. The energy-deprivation diet caused a significant reduction in the number of Entodinium, Eodinium, Isotricha, Dasytricha, Eremoplastron, Eudiplodinium, Metadinium, Charonina, Ostracodinium, and Epidinium protozoa. There was no effect of the time of sampling in both groups on the total number of ciliates in rumen fluid. A higher number of protozoan forms in binary division were recorded in the control group, at the 6th and 9th hours after feeding (PForam utilizados 10 novilhos mestiços com cânula ruminal, distribuídos em dois grupos: no grupo controle (C; n=4 receberam dieta balanceada para ganho diário de 900g; no grupo tratado com carência pronunciada de energia (CP; n=6, receberam dieta com 30% a menos do nível de mantença em energia. Após 140 dias sob esses regimes de alimentação, foram coletadas amostras do fluido ruminal e urina, para realização de provas bioquímicas e funcionais, antes e às 1, 3, 6 e 9 horas após o fornecimento do alimento. A carência energética resultou em diminuição significativa na quantidade dos protozoários Entodinium, Eodinium, Isotricha, Dasytricha, Eremoplastron, Eudiplodinium, Metadinium, Charonina, Ostracodinium e Epidinium. Não houve efeito da hora de coleta sobre o total de ciliados nos grupos C e CP. Maior número de formas em divisão binária foi registrado no grupo C, na sexta e nona horas pós-alimentação (P<0,019. Observaram-se altas correlações positivas entre a contagem total de protozoários e a fermentação de glicose, am

  12. Pathological Studies on the Protective Effect of a Macrolide Antibiotic, Roxithromycin, against Sulfur Mustard Inhalation Toxicity in a Rat Model

    Science.gov (United States)

    2011-01-01

    bronchiolar exudates, bronchial/ bronchiolar epithelial necrosis, bronchial/bronchiolar neutro- phil infiltrates, bronchial associated lymphoid tissue ( BALT ...bronchial neutro- phil infiltrates. SM exposure resulted in higher scores in all areas, but with only minimal differences in BALT necrosis, alveolar

  13. Bronchoalveolar sublineage specification of pluripotent stem cells: effect of dexamethasone plus cAMP-elevating agents and keratinocyte growth factor.

    Science.gov (United States)

    Katsirntaki, Katherina; Mauritz, Christina; Olmer, Ruth; Schmeckebier, Sabrina; Sgodda, Malte; Puppe, Verena; Eggenschwiler, Reto; Duerr, Julia; Schubert, Susanne C; Schmiedl, Andreas; Ochs, Matthias; Cantz, Tobias; Salwig, Isabelle; Szibor, Marten; Braun, Thomas; Rathert, Christian; Martens, Andreas; Mall, Marcus A; Martin, Ulrich

    2015-02-01

    Respiratory progenitors can be efficiently generated from pluripotent stem cells (PSCs). However, further targeted differentiation into bronchoalveolar sublineages is still in its infancy, and distinct specifying effects of key differentiation factors are not well explored. Focusing on airway epithelial Clara cell generation, we analyzed the effect of the glucocorticoid dexamethasone plus cAMP-elevating agents (DCI) on the differentiation of murine embryonic and induced pluripotent stem cells (iPSCs) into bronchoalveolar epithelial lineages, and whether keratinocyte growth factor (KGF) might further influence lineage decisions. We demonstrate that DCI strongly induce expression of the Clara cell marker Clara cell secretory protein (CCSP). While KGF synergistically supports the inducing effect of DCI on alveolar markers with increased expression of surfactant protein (SP)-C and SP-B, an inhibitory effect on CCSP expression was shown. In contrast, neither KGF nor DCI seem to have an inducing effect on ciliated cell markers. Furthermore, the use of iPSCs from transgenic mice with CCSP promoter-dependent lacZ expression or a knockin of a YFP reporter cassette in the CCSP locus enabled detection of derivatives with Clara cell typical features. Collectively, DCI was shown to support bronchoalveolar specification of mouse PSCs, in particular Clara-like cells, and KGF to inhibit bronchial epithelial differentiation. The targeted in vitro generation of Clara cells with their important function in airway protection and regeneration will enable the evaluation of innovative cellular therapies in animal models of lung diseases.

  14. Functional live cell imaging of the pulmonary neuroepithelial body microenvironment.

    Science.gov (United States)

    De Proost, Ian; Pintelon, Isabel; Brouns, Inge; Kroese, Alfons B A; Riccardi, Daniela; Kemp, Paul J; Timmermans, Jean-Pierre; Adriaensen, Dirk

    2008-08-01

    Pulmonary neuroepithelial bodies (NEBs) are densely innervated groups of neuroendocrine cells invariably accompanied by Clara-like cells. Together with NEBs, Clara-like cells form the so-called "NEB microenvironment," which recently has been assigned a potential pulmonary stem cell niche. Conclusive data on the nature of physiological stimuli for NEBs are lacking. This study aimed at developing an ex vivo mouse lung vibratome slice model for confocal live cell imaging of physiological reactions in identified NEBs and surrounding epithelial cells. Immunohistochemistry of fixed slices demonstrated that NEBs are almost completely shielded from the airway lumen by tight junction-linked Clara-like cells. Besides the unambiguous identification of NEBs, the fluorescent dye 4-Di-2-ASP allowed microscopic identification of ciliated cells, Clara cells, and Clara-like cells in live lung slices. Using the mitochondrial uncoupler FCCP and a mitochondrial membrane potential indicator, JC-1, increases in 4-Di-2-ASP fluorescence in NEB cells and ciliated cells were shown to represent alterations in mitochondrial membrane potential. Changes in the intracellular free calcium concentration ([Ca2+](i)) in NEBs and surrounding airway epithelial cells were simultaneously monitored using the calcium indicator Fluo-4. Application (5 s) of 50 mM extracellular potassium ([K+](o)) evoked a fast and reproducible [Ca2+](i) increase in NEB cells, while Clara-like cells displayed a delayed (+/- 4 s) [Ca2+](i) increase, suggestive of an indirect, NEB-mediated activation. The presented approach opens interesting new perspectives for unraveling the functional significance of pulmonary NEBs in control lungs and disease models, and for the first time allows direct visualization of local interactions within the NEB microenvironment.

  15. PROCEDIMIENTO PARA EL CONTROL DE GESTIÓN EN LA EMPRESA CAMPISMO POPULAR DE VILLA CLARA / PROCEDURE FOR CONTROLLING IN VILLA CLARA CAMPISMO POPULAR ENTERPRISE

    Directory of Open Access Journals (Sweden)

    Ariel Espino-Valdés

    2013-05-01

    Full Text Available Actualmente, el control de gestión tiene un papel decisivo como perspectiva de dirección en las empresas cubanas, ya que se optimiza el conocimiento de los trabajadores en función de los resultados de la organización. Se han introducido distintos enfoques en este sentido, pero en pocas empresas se han logrado conectar los objetivos estratégicos con las metas a alcanzar. El presente trabajo tiene como objetivo mostrar un procedimiento para implementar el control de gestión en las Empresas de Campismo Popular, que permita la utilización de los inductores de cambio para el alineamiento de la gestión de los procesos con la estrategia establecida, bajo un enfoque sistémico y proactivo, profundizando en el diagnóstico del clima organizacional. El trabajo muestra como resultado fundamental el diseño de un procedimiento de carácter integrador con enfoques modernos e indicadores de gestión que permitirán el análisis oportuno del cumplimiento de los objetivos.ABSTRACTAt present, the controlling plays an important role as directing philosophy in Cuban enterprises, optimizing the knowledge of employees in terms of results of the organization. Different approaches have been introduced on this direction but it has not been possible to link the strategic objectives with the goals to reach. The current investigation aims at developing a control system of management in Campismo Popular enterprises, with the purpose of using the inductors of change to align the management of processes with the established strategy, under a systemic and proactive approach, deepening in the organizational environment. This work shows important results like the design of a procedure with integrated character, with modern approaches and indicators of management, which will permit the opportune analysis of the fulfillment of objectives

  16. Repair of tracheal epithelium by basal cells after chlorine-induced injury

    Science.gov (United States)

    2012-01-01

    Background Chlorine is a widely used toxic compound that is considered a chemical threat agent. Chlorine inhalation injures airway epithelial cells, leading to pulmonary abnormalities. Efficient repair of injured epithelium is necessary to restore normal lung structure and function. The objective of the current study was to characterize repair of the tracheal epithelium after acute chlorine injury. Methods C57BL/6 mice were exposed to chlorine and injected with 5-ethynyl-2′-deoxyuridine (EdU) to label proliferating cells prior to sacrifice and collection of tracheas on days 2, 4, 7, and 10 after exposure. Airway repair and restoration of a differentiated epithelium were examined by co-localization of EdU labeling with markers for the three major tracheal epithelial cell types [keratin 5 (K5) and keratin 14 (K14) for basal cells, Clara cell secretory protein (CCSP) for Clara cells, and acetylated tubulin (AcTub) for ciliated cells]. Morphometric analysis was used to measure proliferation and restoration of a pseudostratified epithelium. Results Epithelial repair was fastest and most extensive in proximal trachea compared with middle and distal trachea. In unexposed mice, cell proliferation was minimal, all basal cells expressed K5, and K14-expressing basal cells were absent from most sections. Chlorine exposure resulted in the sloughing of Clara and ciliated cells from the tracheal epithelium. Two to four days after chlorine exposure, cell proliferation occurred in K5- and K14-expressing basal cells, and the number of K14 cells was dramatically increased. In the period of peak cell proliferation, few if any ciliated or Clara cells were detected in repairing trachea. Expression of ciliated and Clara cell markers was detected at later times (days 7–10), but cell proliferation was not detected in areas in which these differentiated markers were re-expressed. Fibrotic lesions were observed at days 7–10 primarily in distal trachea. Conclusion The data are

  17. Repair of tracheal epithelium by basal cells after chlorine-induced injury

    Directory of Open Access Journals (Sweden)

    Musah Sadiatu

    2012-11-01

    Full Text Available Abstract Background Chlorine is a widely used toxic compound that is considered a chemical threat agent. Chlorine inhalation injures airway epithelial cells, leading to pulmonary abnormalities. Efficient repair of injured epithelium is necessary to restore normal lung structure and function. The objective of the current study was to characterize repair of the tracheal epithelium after acute chlorine injury. Methods C57BL/6 mice were exposed to chlorine and injected with 5-ethynyl-2′-deoxyuridine (EdU to label proliferating cells prior to sacrifice and collection of tracheas on days 2, 4, 7, and 10 after exposure. Airway repair and restoration of a differentiated epithelium were examined by co-localization of EdU labeling with markers for the three major tracheal epithelial cell types [keratin 5 (K5 and keratin 14 (K14 for basal cells, Clara cell secretory protein (CCSP for Clara cells, and acetylated tubulin (AcTub for ciliated cells]. Morphometric analysis was used to measure proliferation and restoration of a pseudostratified epithelium. Results Epithelial repair was fastest and most extensive in proximal trachea compared with middle and distal trachea. In unexposed mice, cell proliferation was minimal, all basal cells expressed K5, and K14-expressing basal cells were absent from most sections. Chlorine exposure resulted in the sloughing of Clara and ciliated cells from the tracheal epithelium. Two to four days after chlorine exposure, cell proliferation occurred in K5- and K14-expressing basal cells, and the number of K14 cells was dramatically increased. In the period of peak cell proliferation, few if any ciliated or Clara cells were detected in repairing trachea. Expression of ciliated and Clara cell markers was detected at later times (days 7–10, but cell proliferation was not detected in areas in which these differentiated markers were re-expressed. Fibrotic lesions were observed at days 7–10 primarily in distal trachea. Conclusion

  18. Making Health Easier: Tobacco Free Living in Santa Clara, CA PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2013-05-21

    Instead of walking through clouds of smoke, students in a California community college can now breathe clean air. A college health nurse made it happen with the help of students, faculty, and partners.  Created: 5/21/2013 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 5/21/2013.

  19. Low-temperature geothermal assessment of the Santa Clara and Virgin River Valleys, Washington County, Utah

    Energy Technology Data Exchange (ETDEWEB)

    Budding, K.E.; Sommer, S.N.

    1986-01-01

    Exploration techniques included the following: (1) a temperature survey of springs, (2) chemical analyses and calculated geothermometer temperatures of water samples collected from selected springs and wells, (3) chemical analyses and calculated geothermometer temperatures of spring and well water samples in the literature, (4) thermal gradients measured in accessible wells, and (5) geology. The highest water temperature recorded in the St. George basin is 42/sup 0/C at Pah Tempe Hot Springs. Additional spring temperatures higher than 20/sup 0/C are at Veyo Hot Spring, Washington hot pot, and Green Spring. The warmest well water in the study area is 40/sup 0/C in Middleton Wash. Additional warm well water (higher than 24.5/sup 0/C) is present north of St. George, north of Washington, southeast of St. George, and in Dameron Valley. The majority of the Na-K-Ca calculated reservoir temperatures range between 30/sup 0/ and 50/sup 0/C. Anomalous geothermometer temperatures were calculated for water from Pah Tempe and a number of locations in St. George and vicinity. In addition to the known thermal areas of Pah Tempe and Veyo Hot Spring, an area north of Washington and St. George is delineated in this study to have possible low-temperature geothermal potential.

  20. A senior manager with a knowledge management portfolio: the Santa Clara County experience.

    Science.gov (United States)

    Lindberg, Arley

    2012-01-01

    The agency director sought to create a systematically coordinated department that utilizes knowledge management strategies to promote evidence-informed practice. In his view, the organization was not providing needed information or organizational supports for practitioners to use knowledge effectively. To address this issue, he created a Director of Development and Operational Planning (DDOP) position with the responsibility to build structures and facilitate processes that support knowledge management. The DDOP oversees research and planning, government relations, legislative development and support, Board of Supervisors communications, staff development and training, community contracts, public information and in-house communication. The DDOP is reorganizing units under her supervision to create a knowledge management matrix that will implement new knowledge sharing strategies related to evaluation, contracts, legislation, organizational development, policy and planning, and staff development. The case study describes challenges and strategies related to: government regulations, size and complexity of the agency, staff resistance, and the developmental nature of the process. Copyright © Taylor & Francis Group, LLC

  1. International Symposium on Electromagnetic Compatibility, 24th, Santa Clara, CA, September 8-10, 1982, Proceedings

    Science.gov (United States)

    The incorporation of electromagnetic compatibility (EMC), and the prevention of electromagnetic interference (EMI) in the design of electronic devices are considered in individual contributions. Topics covered include EMC regulation, spectrum management, equipment shielding, system analysis and modeling, computer-systems EMC, measurement techniques, composite materials in EMC design, personnel electrostatic discharge, EMC testing, electromagnetic environments, and coupling and shielding analysis. Discussion is also presented on EMI design, industrial and commercial EMC, lightning and electromagnetic pulses (including aircraft-design problems), and naval EMC applications. No individual items are abstracted in this volume

  2. Vintage Glass by the Bay - The 1994 ATS Convention in Santa Clara, CA

    Science.gov (United States)

    Breyer, Walter

    Papers included: "Pauper and the Prince: George Willis Ritchey, George Ellery Hale and the Big American Telescopes" by Dr. Donald Osterbrock; "The Observatory that Never Was" by Dr. Eugene Rudd; "Who Did Make Sproul Observatory's 6" Refractor?" by Bart Fried; "Through the Looking Glass" [early tele. makers: Guinand, Feil, Mantois, Chance, Fraunhofer & Short] by Shiloh Unruh [Lick]; "ATMs and the ATS" by Peter Abrahams; "The Telescopes of J.H. Reynolds of Birmingham, England: One of the Great Amateur-Professionals" by Dr. Ron Maddison; "Cleaning and Repairing the Chabot Observatory's 20" [Brashear] Lens" by Bob Schalck [Chabot Obs.]; and "How Fitz Fits: The Roles of Telescopes and Their Makers in 19th Century America" by Norm Sperling. Visits were made to Ricard Observatory [Swift 16" Clark, 8" Fauth & 4" Secretan et Lerebours], Lick Obs. [36" Clark], & Chabot Obs. [20" Brashear].

  3. Water quality of the Lexington Reservoir, Santa Clara County, California, 1978-80

    Science.gov (United States)

    Iwatsubo, R.T.; Sylvester, M.A.; Gloege, I.S.

    1988-01-01

    Analysis of water samples from Lexington Reservoir and Los Gatos Creek upstream from the reservoir from June 1978 through September 1980 showed that water generally met water-quality objectives identified by California Regional Water Quality Control Board, San Francisco Bay Region. Water-temperature profiles show that Lexington Reservoir is a warm monomictic lake. During summer, dissolved-oxygen concentrations generally were not reduced below 5.0 mg/L in the hyplimnion; only once during the study did bottom waters become anoxic. Water transparency decreased with depth. The euphotic zone ranged from 1.0 to 5.4 m, depending on suspended solids and algae, and was greater in summer than in spring. Calcium and bicarbonate were dominant ions at all stations except during spring, following the rainy season, when waters were a mixed cation bicarbonate type. Nitrogen concentrations were greater in samples from reservoir stations than in those from Los Gatos Creek, with most of the nitrogen in ammonia and organic forms. The amount of dissolved nitrate appeared to be related to phytoplankton abundance. Phosphorus and trace-element concentrations were low at all stations. Estimates of net primary productivity and Carlson 's trophic-state index, based on chlorophyll-a concentrations, indicated that reservoir classification ranges from oligotrophic to mesotrophic. Blue-green algae generally were predominant in reservoir samples. (USGS)

  4. Santa Clara Computer And High Technology Law Journal; Vol. 11, No. 1

    Science.gov (United States)

    1995-03-01

    SINCE SURFACE TRANSPORTATION MOVES PEOPLE & GOODS ALONG PUBLIC ROADS & TRANSIT SYSTEMS, IT MAY SEEM ODD TO BE CONCERNED ABOUT PRIVACY IN SUCH A HIGHLY PUBLIC CONTEXT. AND YET, RESPECTING PRIVACY WILL BE IMPORTANT, AS ADVANCED TECHNOLOGIES IMPROVE HIG...

  5. South Fork of the Santa Clara River, Santa Clarita Valley, California. Supplement.

    Science.gov (United States)

    1985-01-01

    well as from the Miocene Topanga sandstone and the Modelo shale found near the crest of the Santa Susana Mountains in Aliso and Rice Canyons. Fossil...ground squirrel. The woodland habitat provides excellent habitat for wildlife in the project area, particularly hole nesting species such as plain...coyote (Canis latrans), dusky-footed woodrat (Neotoma fuscipes), and California ground squirrel. The woodland habitat provides excellent habitat for

  6. Crystallization kinetic of clear burning kaolinitic clay; Cinetica de cristalizacao de argila caulinitica de queima clara

    Energy Technology Data Exchange (ETDEWEB)

    Silva, R.A.; Medeiros, J.; Teixeira, S.R. [Universidade Estadual Paulista (DFQB/FCT/UNESP), Presidente Prudente, SP (Brazil). Fac. de Ciencias e Tecnologia. Dept. de Fisica, Quimica e Biologia

    2009-07-01

    It was studied the crystallization kinetics of one caulinitic clay of clear burning, from Teodoro Sampaio (SP) region. Differential thermal analysis (DTA), X-ray diffraction (XRD) and X-ray fluorescence (XRF) had been used in the development of the work. Non-isothermal methods had been used for attainment of the kinetic data and in the determination of the crystallization mechanisms. The exothermic peak associate to the crystallization is located between 900 and 1000 deg C, varying in accordance with the heating rate of the sample. The activation energies were taken using the data of thermal analysis (DTA) and the methods of Kissinger (700 kJ/mol) and Ligero (721 kJ/mol). The Avrami constant n was calculated using Ligero and the m parameter was obtained using Matusita method and the n and E of Ligero. Both values (1.17) are close to 1, indicating growth in two dimensions with morphology of plates and nucleation of surface as dominant mechanism. (author)

  7. Predicting pear (cv. Clara Frijs) dry matter and soluble solids content with near infrared spectroscopy

    DEFF Research Database (Denmark)

    Travers, Sylvia; Bertelsen, Marianne; Petersen, Karen

    2014-01-01

    Regression models for predicting preharvest dry matter (DM) and soluble solids content (SSC), based on two spectral ranges (680-1000 nm and 1100-2350 nm), were compared. Models based on longer NIR spectra were more successful for both parameters (DM/SSC: R2 = 0.78-0.84; RMECV = 0.78/0.44; LVs = 6...

  8. International Powered Lift Conference and Exposition, Santa Clara, CA, Dec. 7-10, 1987, Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    1988-01-01

    The present conference on VTOL, STOVL and V/STOL fixed-wing aircraft powered lift discusses hot gas recirculation in V/STOL, flight testing of a single-engine powered lift aircraft, RAF experience with VTOL, near-term improvements of the AV-8B Harrier II, recent advancements in thrust augmentation, lift ejectors for STOVL combat aircraft, the correlation of entrainment and lift enhancement for a two-dimensional propulsive wing, the thrust efficiency of powered lift systems, and flight propulsion control integration for V/STOL aircraft. Also discussed are VSTOL design implications for tactical transports, the numerical investigation of a jet in ground effect with a cross flow, the NASA supersonic STOVL propulsion technology program, the aeroacoustics of advanced STOVL aircraft plumes, powered lift transport aircraft certification criteria status, the application of vectored thrust V/STOL experience in supersonic designs, wave drag and high speed performance of supersonic STOVL fighter configurations, and the impact of bypass ratio on thrust-to-weight for V/STOL.

  9. DIGITAL FLOOD INSURANCE RATE MAP DATABASE, SANTA CLARA COUNTY, CALIFORNIA, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

  10. Edificio para Memorex Corporation - Santa Clara – California (EE.UU.

    Directory of Open Access Journals (Sweden)

    Leland King y Asociados, Arquitectos

    1973-09-01

    Full Text Available This building has the following features: — it makes future possible extensions; — uses space gradually, according to work arrangement; — reduces to a minimum distances between car parks and places of work; — centralizes dining-room installations for employees; — achieves great economy, thanks to the modulation of design; — takes maximum advantage of each space, for using easily removable partitions, and — increases productivity and personnel performance, due to the suitable conditioning of the atmosphere. The corporation is complemented by a carefully studied series of access roads, car parks and gardens, which at the same time as they intercommunicate the different areas, adapt the outer and indoor atmosphere, forming a harmonious whole.Este edificio reúne las siguientes características: — permite la posibilidad de futuras ampliaciones; — utiliza progresivamente los espacios, de acuerdo con la ordenación de los trabajos; — minimiza las distancias entre los aparcamientos y los puestos de trabajo; — centraliza las instalaciones de comedor para los empleados; — consigue una gran economía, gracias a la modulación del diseño; — potencial iza usos en cada espacio, por la utilización de particiones fácilmente desplazables, y — aumenta la productividad y rendimiento del personal, por el tratamiento adecuado del ambiente. La corporación está complementada por una estudiada serie de accesos, estacionamientos y espacios ajardinados, que al mismo tiempo que comunican entre sí las diferentes zonas, adecúan el ambiente exterior con el interior, formando un conjunto homogéneo.

  11. 75 FR 79013 - Santa Clara Valley Habitat Conservation Plan and Natural Community Conservation Plan, CA...

    Science.gov (United States)

    2010-12-17

    ... manage anticipated growth and development. The Draft HCP/NCCP will also provide a coordinated process for... compliance with the CEQA, but all Applicants share the CEQA Lead Agency role. The California Department of... implement a habitat conservation plan and natural communities conservation plan that is similar to the Draft...

  12. From New Haven to Santa Clara: A Historical Perspective on the Milgram Obedience Experiments

    Science.gov (United States)

    Blass, Thomas

    2009-01-01

    This article traces the history of obedience experiments that have used the Milgram paradigm. It begins with Stanley Milgram's graduate education, showing how some aspects of that experience laid the groundwork for the obedience experiments. It then identifies three factors that led Milgram to study obedience. The underlying principles or messages…

  13. An electron microscopic study of the phosphatases in the ciliate Balantidium coli.

    Science.gov (United States)

    Skotarczak, B; Kołodziejczyk, L

    2005-11-01

    The localisation and activity of D glucose-6-phosphatase (G-6-Pase) and alkaline phosphatase (AlP) in the trophozoites of Balantidium coli isolated from pig intestine content were investigated using ultrastructural and cytochemical methods. The activity of G-6-Pase was demonstrated on the membranes of the endoplasmic reticulum, particularly in the cortical part of the trophozoites. In addition, the product of the reaction to G-6-Pase was concentrated in the vesicular structures, which were distributed along the reticular membranes. These structures were described as vesicles similar to glycosomes, containing enzymes of glycogenolysis. It is very likely that hydrolases in B. coli are formed on the rough reticular membranes without the involvement of cisterns of the Golgi complex. The ultrastructural deposits of the reaction to G-6-Pase and AlP in the trophozoites of B. coli described here indicate that some membranes of the rough endoplasmic reticulum and small vacuoles with a strong reaction to these enzymes can play a similar role to the Golgi complex.

  14. Amebae and ciliated protozoa as causal agents of waterborne zoonotic disease.

    Science.gov (United States)

    Schuster, Frederick L; Visvesvara, Govinda S

    2004-12-09

    The roles free-living amebae and the parasitic protozoa Entamoeba histolytica and Balantidium coli play as agents of waterborne zoonotic diseases are examined. The free-living soil and water amebae Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris are recognized etiologic agents of mostly fatal amebic encephalitides in humans and other animals, with immunocompromised and immunocompetent hosts among the victims. Acanthamoeba spp. are also agents of amebic keratitis. Infection is through the respiratory tract, breaks in the skin, or by uptake of water into the nostrils, with spread to the central nervous system. E. histolytica and B. coli are parasitic protozoa that cause amebic dysentery and balantidiasis, respectively. Both intestinal infections are spread via a fecal-oral route, with cysts as the infective stage. Although the amebic encephalitides can be acquired by contact with water, they are not, strictly speaking, waterborne diseases and are not transmitted to humans from animals. Non-human primates and swine are reservoirs for E. histolytica and B. coli, and the diseases they cause are acquired from cysts, usually in sewage-contaminated water. Amebic dysentery and balantidiasis are examples of zoonotic waterborne infections, though human-to-human transmission can occur. The epidemiology of the diseases is examined, as are diagnostic procedures, anti-microbial interventions, and the influence of globalization, climate change, and technological advances on their spread.

  15. Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

    DEFF Research Database (Denmark)

    Jørgensen, Louise von Gersdorff; Kania, Per Walter; Rasmussen, Karina Juhl

    2017-01-01

    responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may......The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two...... produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody...